LOCUS       DAOQOU010000001        55073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_222_length_55073_cov_5.718728, whole genome shotgun sequence.
ACCESSION   DAOQOU010000001 DAOQOU010000000
VERSION     DAOQOU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 55073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55073
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_55073_cov_5.718728"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PLM90_00005"
     CDS             <1..1499
                     /locus_tag="PLM90_00005"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ87773.1"
                     /translation="TQDIQHPYATSILVDGERIIAVGDETLLLEAGDIQKIDIGGRHI
                     FPGLHDAHIHIWKVGNLLTNMLDLRGVGSIDEMQQRLSDYARSHPELTWIQARGFNEV
                     IFPDKRIPNKLDIDAVISDRPVVITRTCAHQVVANSKALLLAGLDKNMQPVPGGEIKY
                     LASGELSGHFTETAIGLILKAVPKYTAAELRNMVLTAQDAFLKLGITAACDPAVDRDL
                     LEVYKQMDADGELRMRINAIPIRVPDGSQKIYPNPQHYYSSHLVVNTVKFFADGGLSG
                     KTAALRLPYLNDTSKGVMRLEKEQFYQLALESQEAGFKIATHAIGDAAVDLVLQVYDN
                     LPLSNIEHRLEHVGFVDDNHLDIMYRRKVSAVMQPIFIRELGKNFIVSLDKKRLDSIY
                     PIHTIAAHGINVALSTDAPVVRSFDPRTNMLAAQTRETEEGIVIGASEILDHKQSTAM
                     YTHGSSVAVGNVANIGTIAKGRYCDLVIHDFPDLSGSAEVWLSGSKIR"
     gene            1598..3466
                     /locus_tag="PLM90_00010"
     CDS             1598..3466
                     /locus_tag="PLM90_00010"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87774.1"
                     /translation="MTIRYALTSLGKPLMAALAVAGGLTVSAQSYIQSTCDYAPLTTE
                     GTVICLGDDAVSSAIPLGFSFNFYDVPYTDCYVSSNGYLSFAAGLGSACCTGQILPNA
                     TYPASIFFGQEDLDPNSCIDGDISYYTTGAPGSQIFVLSFVDVPHYPGPEGTFPVTVQ
                     VQLYEGTNEVRIVTTQWNSDGGAATMGLNENGSNADPVSGRNSEVWSAFDECISFVPN
                     TEPTGGCMDAAAINYDPGADFDDGTCFYGYIASTCDYSPMATEGTIVCLGDDQVSTAI
                     DMGFDFPFYGTPHNQAYISSNGFVTFQPGMGSGCCTGQILPNATYQNTIFIGQEDLDP
                     NTCVDGEINYYTTGAPGSQVFVVNFIGVPHYPGPAGTFNITVQLQCYEATGEIRIVTT
                     EWNSDGGAATMGLNYNGTYADWVPGRNSSVWDAFDECYSWMPLTEPLPVCDAPGGLFA
                     DGITTDDAVLHWDAVDGAMNYNVILQNTATGLLKKKVFTTNMAVIENKLSPLTNYAFR
                     VKTGCGVDPENMSDPSPWYYFTTLGRTGEEQATVTLFPNPNNGVFTLNVAGYADNSFN
                     LNIYNTMGAIVYTKTVDITSDNQAVVVSLENVPGGMYQINLSNDQYQINYPVIVTK"
     gene            3729..5786
                     /locus_tag="PLM90_00015"
     CDS             3729..5786
                     /locus_tag="PLM90_00015"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ87775.1"
                     /translation="MMNMLSYAKTWLTLGVVVVTTGLASAQYCTPVYTTGTADGDYIN
                     GVTVGAIANTGSGAAITGVGYSDYTALSSDLTVGSTYTMTLDNNPSWSQTYTAWIDYD
                     QDEVFETTEVLGGVGLSAGASGTVTFTVPVGTPGGSTRMRVRCIYPSGLAIPQDPCLS
                     ATYGEAEDYTINIVGGAANDVGVIDITNPISAVDLGFENVTITIRNYGSEPASGFNVY
                     FQVDGGLVTGATYGGTIDAYSTASYTFGDTWDFTADGCYDITAWTDYELDEIADNDDY
                     TETVCNLGPITGTGAWYIYSNSTGGEPWFTTTNSTAMTTVFGAEGTGWNRAFFETLDP
                     LSIFNSDNCFVFIDGSDAQANELETFLTANMSTIENWVSSGGKLLLNSAPNEGDGMSF
                     GFGGTTLTYSYYTSNATAVDALHPIFNGPFTPAGTDYTGTSFGHAIVSGVDLSNVMVD
                     LFNPSAVVLAEKTWGDGRVMFGGMTTNNFHSPLTNAANLRANIIAYMSCMEFVICLPP
                     GGLYADGITTDDAVLHWDVVDGALNYNVILQNTATGLLKKKVFATNMAVIENKLSPLT
                     NYAFRVKTGCGVDPDIVSMASEWYYFTTLGREGVNEAAVSLYPNPNNGTFTINVAGYE
                     GNSFNLNVVSATGQIVYTKTVDVTGANYAEQIALDNIPAGMYQVNLSNAQYQINYAIV
                     VTK"
     gene            6069..8123
                     /locus_tag="PLM90_00020"
     CDS             6069..8123
                     /locus_tag="PLM90_00020"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ87776.1"
                     /translation="MKNMLKHLRTGLVLAAVIGSSIAAKAQYCTPTYTTGTAEGDYIN
                     GVTVGTIANTFSGAAITGVGYSDYTFLSTDLNIGDAYTMTCNNNPSWSQTYTAWIDYD
                     HDNTFETSEVLGNLSLSASATGSFSFTVPVTAMSGTTRMRVRCIYPSGLVTPLDPCAL
                     ATYGETEDYTVNLLGGAMNDVGVIDITNPTTGPGLGMEDVIVTVRNYGSEPASGFTVS
                     YQVDGGLVTAGFYGGTIAPFSTGSWTAPEGWDFSAEGCYNITAWTTLAGDEDPGNDSY
                     TEEVCHTGAVTGTGAIYMLSNTTGGEPWFTTNNSTAMTTVFGAEGTGWNKNYYETAPA
                     ATVFTPDNCFVFMEGSDGHANELETYLTANMAVIESWVSSGGKLLLNAAPNEGDGMSF
                     GFGGTSLVYSYFTSTANGVGGHPIFSGPYTPAGTTYTGTSFGHARITGTGLTNVMVDA
                     AAPSNVVLAEKTWGGGIVMFGGMTTDNWHDPDPNAANLRANIFSYLSCEVVTDCPAPV
                     GLGADAITGTSAHLFWDPSLNSGGYHLSVYKTDETLVLKKKVLGGATDWTVTGLTPGT
                     DYAFHVRSICTGLGMASDISYHYYFTTPARLGDENTNVVLFPNPNTGTFDIALNGYEG
                     QQVNIQIVSALGQTVYNNTVNVNATGFTQNVNLNNAAAGMYYVNILTDKGVMNYQVVI
                     TD"
     gene            complement(8175..8771)
                     /locus_tag="PLM90_00025"
     CDS             complement(8175..8771)
                     /locus_tag="PLM90_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HMZ87777.1"
                     /translation="MELPVMEQFYTIQGEGFHQGRAAYFIRLGGCDVGCVWCDVKESW
                     DVDAHPKLSVSSLVNNAAAFASKIVVITGGEPLMHNLSALTNELHEKGLNTHLETSGA
                     HPLSGIWDWITFSPKKFKSPLASVAAQANELKVVINHKSDLVFAEMHAATVSESCLLY
                     LQPEWEKRDSMMPLITEYVRTNPQWRISLQTHKYLQIP"
     gene            complement(8805..9692)
                     /locus_tag="PLM90_00030"
     CDS             complement(8805..9692)
                     /locus_tag="PLM90_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HMZ87778.1"
                     /translation="MAQPIILDGKKLSTEIQDEIRKKIDIRRTAGLKIPHLAAVLVGN
                     DGASTTYVNSKIRLCKSVGMQSTLIHLDASTSEEELLRVVHDLNTNPDIDGFIVQLPL
                     PKHIQEMHIVEAIDPSKDVDGFHPVNLGRMQLGLPAFISATPAGIMTMLSRYHIDTAG
                     KHCVVIGRSNNVGTPMSLLLSRNTNPGNCTVTLCHSRTKNLTEICRSADIIIAALGKP
                     KFVTGDMVKEGAVVIDIGISRVDADNEKGYLILGDVDFPNVAPKCSAISPVPGGVGLM
                     TVVSLIMNTMQAAESRDKL"
     gene            complement(9794..11584)
                     /gene="lepA"
                     /locus_tag="PLM90_00035"
     CDS             complement(9794..11584)
                     /gene="lepA"
                     /locus_tag="PLM90_00035"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HMZ87779.1"
                     /translation="MEHIRNFCIIAHIDHGKSTLADRLLEVTKTVSEREAQAQVLDDM
                     DIERERGITIKSHAIQMDFRYEGKQYVLNLIDTPGHVDFSYEVSRSIAACEGALLLVD
                     ATQGIQAQTISNLYLAIEHDLEIIPVVNKIDMDGAMVDEVKDQIVDLIGCKREEILEA
                     SGKTGVGIDSIFDAIIHRIPSPKGNVDAPLQALIFDSVFNSYRGVVAYYRIFQGTIKK
                     GDKIKFVNTQNNYEANEVGILRLQYEPRSEVKAGDVGYVITGIKEAKEIKVGDTITLR
                     DNPCSNEVAGFKDVKPMVFAGIYPIDNDDFADLRDSLEKLQLNDASLVFEPESSVALG
                     FGFRCGFLGLLHLEIVQERLEREFDQNVIITVPNVSFDCYTTAGEKIVVHNPTDMPDP
                     GSIDKIEEPYIRSQIITAPDYIGAIMKLCMDKRGILKNQVYLTTNRVELTWEMPLAEI
                     VFDFYDKLKSISRGYASFDYELIDYREGDLIKLDIKLNGEQVDAFSALIHRDKAYDFG
                     SRLCGKLKDLLPKQQFVIAIQAAIGAKIIARETISAMRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMRQIGTVEVPQEAFLAVLKLNE"
     gene            11698..12261
                     /locus_tag="PLM90_00040"
     CDS             11698..12261
                     /locus_tag="PLM90_00040"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ87780.1"
                     /translation="MQRKKRPTMTHNYQTASPVKRISAFAIDTILVGVVSAAFLFVTM
                     GKTPAKLQASGDEGRPSIADKYQFFAYAEPIVNDPNRAIYIENFLKKYKMQAMVGLFL
                     LPLLYYVLFEGLWGATIGKLLTGIRVRRKDGGKISIGTAFLRHIGRIISTMIFMLGYL
                     LAFIDGKRQTLHDKIANTMVLKKGTGI"
     gene            complement(12262..14349)
                     /locus_tag="PLM90_00045"
     CDS             complement(12262..14349)
                     /locus_tag="PLM90_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87781.1"
                     /translation="MKIFHPHIFICTSFMMMFSFAHAQENKGEGKLLEDDIIEGAAEK
                     TDNADLSIDTYQENLNYLLQNPISINTASYIELRNSGLFTEVQIRDLRRHIESYGPLL
                     NLYELQTIPSFYPEDIARILPFITLNQVSRDKVAFIDQLYLGDYQYYTRLSRYLETQD
                     GYVADSAGNTSYAGNNLRVYNRFRYNYNNKLSYGFTAEKDAGEAVFGPTQPYGFDYYS
                     AHFFKKGTGTMRALALGDFELRIGQGLMMWSGFGFGKSVFPLAIRRAGPVLDSYTSTN
                     ENRFLRGAGATLAYGRWQFTPFISYKSIDANVALLDTVSDEVIQVSAIDESGLHRTES
                     ELERKDALKELMGGFDLTWYKDQVNIGFSAIGYHYSAPLQPTISPYEIYNFTGQQLIN
                     FGTHYNFLWRNMLLFGETAMSDNGKAGTLNGVIIPLDPKMDIAILHRYFSPDYQTLYA
                     ATFSDATLPQNENGTYFGTEIKPIKGWKLSGYVDLYKHQWLEYQTDAPGYGTDLLAQL
                     TWQPSRSFNTYVRYKRENSDRNSTDDIIDAENQLNVVSDLMRSSIRWHADYDVNKAIT
                     VKSRIEVSFYEETYGIPERGYLVFQDFNYKPLSSPIGFATRFAIFNTDSYNTRIYAYE
                     TEVLYAYSIVGLSGRGTRTYLMFTWSPFKWMDVWARVANTWYSGDEPLGSGDNTFPGN
                     TRSDAKLQLRIKW"
     gene            complement(14363..15181)
                     /locus_tag="PLM90_00050"
     CDS             complement(14363..15181)
                     /locus_tag="PLM90_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87782.1"
                     /translation="MNPGKLLTCAILCISLSVHAQTGYSDAGASANSMGNTGIANPDV
                     FSAQNNQAAMAFAENASVGISTQNYFLMDEGLNAFYAAGIIPVQRAGNFGITAHYTGD
                     ATFNQTKIGLGYGRKLADVLAIGVQLDYVGTNTSEVGNGTAFTFDIGVLYKPTKTLSI
                     GAKAFNPVRVKNGLEYAEELPAIITAGLQWQPSEFVRVCLEGEQNIDQDLRIKTGIEY
                     HIIDVLYLRGGYISNPSMFTTGIGLKLKTFMLDAGAQFHQQLGVTPGVSLQYAF"
     gene            15301..17556
                     /locus_tag="PLM90_00055"
     CDS             15301..17556
                     /locus_tag="PLM90_00055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HMZ87783.1"
                     /translation="MDVNMILIAIPVLGLIALLFTYMKSNWVARQDPGDAKMQGIAGA
                     IADGAMAFLRAEYRILAIFVVIVAILLGLSGASEENSSALVGVSFVVGASCSALAGFI
                     GMRVATKANVRTANAARGGLGKALEIAFSGGSVMGMGVVGLGILGLGTLLLIYSNIYG
                     FDSQEAAIKVINVVTGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHP
                     LNPATIADNVGDNVGDVAGMGADLFESYVGSIVGTMVLGTAFMAGTIGVGTSFMEDGF
                     NGLSAVLLPLVLGGVGILTSIVGTFFVRVKEGGNPQKALNGGALGSSIAMVILSYFII
                     TWMLPEKFTFTDVLYGGKERTVTAIGIFIATVVGLIAGSAIGFITEYYTGTGKKPVMK
                     IVQQSSTGSATNIIAGLSVGMMSTAIPTLIISAAIIVSFMFGGLYGIAIAAVGMLANT
                     GIQLAVDAYGPISDNAGGIAQMADLPKEVRGRTDKLDAVGNTTAAIGKGFAIGSAALT
                     ALALFGAFMQVTHLTQIDISQAHVIAGLFIGAMLPLLFSAMAMQSVGKAAMSMIQEVR
                     RQFNEIPALKAALDAMKRNGDKEVHEWSKADQEIFEAANGKAEYAKCVEISTKAAVKE
                     MVVPGLMAVIVPVIVGFAPKVLGLNTEGHELMGPEMLGGLLAGVTVSGVLMAIFQANA
                     GGAWDNAKKMFEEGVDINGKTYYKGSDPHKAAVVGDTVGDPFKDTSGPSINILIKLMS
                     VVSLVLASLMV"
     gene            17636..19051
                     /locus_tag="PLM90_00060"
     CDS             17636..19051
                     /locus_tag="PLM90_00060"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87784.1"
                     /translation="MRRIFPWVLVCLYPFAMHAQIDTVTLTEDGHFYFENFLPYSAYS
                     SLMNRNGEPYLYAACRELGVVTFDISDPDFPVPVDTIPTTALNNLIPNNISQTGDYLY
                     VATGGFNLIPQKAGLAIMNLADAAHPVITDKWDSVAYNQGAAIAISDGNYAYLGGMDD
                     GLIILDVSNKSNITFKSSILPDPNYPEVPDLFSVPNVRGLALYGTDTLMVAYDAGGLR
                     MLDVSNKSAPIEIGKYADPDIENIAQSAYNNIAIKQHYAYIPVDMCGLLVVDIADSDM
                     STVAWFNPWDCDSTNWNGRPGHTNEVRINEDEDLLFVSGGDSELLIFDISNPATPTLK
                     ATYGVPFDSIVAWSLDVFGNQISLALVDNSFVGFPYYSDVGGIQLLQYEAIHTIDIQS
                     LYKEFKVYPNPSTGVIVIDDIPSGSAMRIMNLQGVICEPEINGNVIDCSSLASGIYIL
                     QLHDIQGRLIGSSKIVLTGVN"
     gene            complement(19349..21436)
                     /locus_tag="PLM90_00065"
     CDS             complement(19349..21436)
                     /locus_tag="PLM90_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ87785.1"
                     /translation="MRKLLLTVAAGVISVSLFAQTAVTAKPHNKAEMMAKFKNAKAVP
                     YQPQVNADLNGTPLNTNTFYAKSGARTGESIGITGYPLMSNSASYDRVRVYDDGAISA
                     IWTGSTVADGSWADRGSFYNHNDGASWGPAPTARVETVRTGFPELLTVMDHEVVIAHD
                     GTNQRLFANASIGGTTWTELPTSGMHHGLWPRAYCPAGTDDIYLVSPNASPVVTINFS
                     RSDDGGATWTVLNDVLPLDSTNCFGALSADAYQIAVDGSNVYILYGTSWTDLVLLTST
                     SNGDPGSWTITTIINTGFCNYQGDLDQTSDVTGDGIADTVETTDGFHEMVLDDAGVVH
                     VWSGYYWLLDDDPATEGWSYFPSLYGLWYWNSTMAAESPMWIDLLVDWDDSGDAFDGI
                     GADLGMYDGVTFTSMPTAAIDEDAGRIYLAYTMPIEYTDYFDDPTVAEAQSFRDLFGV
                     YSDDMGLSWSAPVNMTYTAHDNQEVSFPYAFDRFLNGCMYTIWHQDDEPGTALDVGQV
                     AADVNSIANMQFRCFDEARFNPYPPTAEYDYTPDGTTGLVNFTNLSVDADTYSWDFGD
                     GGSSTAKNPAHVYATSGVFNVCLTAMNKYDDDNACKVIDITVGVVDYALGQALSVYPT
                     PASTNVTVEVNGNFGTLYAEVYNALGERVINTTAFNGSVNFDVTALSAGNYIVKVATV
                     DGKYTSRQISVSK"
     gene            complement(21647..22012)
                     /locus_tag="PLM90_00070"
     CDS             complement(21647..22012)
                     /locus_tag="PLM90_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87786.1"
                     /translation="MAKTTKKAAGKRGRPAGKKAAKKAAPKKAAKKAAPKKAAKKAAP
                     KKKAAKKAAPKKAAKKAAPKKAAKKAAPKKAAKKAAKKAAPKKAAKKAAPKKKAGKRG
                     RPAAKKVAAPAAPANGATN"
     gene            complement(22200..22697)
                     /locus_tag="PLM90_00075"
     CDS             complement(22200..22697)
                     /locus_tag="PLM90_00075"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ87787.1"
                     /translation="MKELLNSYCDYNVWANTAIIQTLSSVSPEIIDKHMGGSFPTIRK
                     TLYHIWDAQVIWLSRLQGSSPSDWPSAMYDDEFAGYDLYFIQQSEDFARFVSSRPEDF
                     FGDMCFYKALNGQEYQTRNGEIILHCMNHGSYHRGQIINYLRMQGVSDLPHTDYIFYS
                     RAKQS"
     gene            complement(22789..24078)
                     /locus_tag="PLM90_00080"
     CDS             complement(22789..24078)
                     /locus_tag="PLM90_00080"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HMZ87788.1"
                     /translation="MKVVCILLSTPIYIGVSAQSDSTMIPEVKKGLVEMVSDPMIRTV
                     QLQYSTLELAYPILPLNTDGQFLTLSFDDLHGGVKDYYYTFTHCDQQWEPSGLTSFDI
                     IDGFDESRISDYQFSFGTLQRYTHYAVSFPNEDVQFRLSGNYIVQVWKDEDRDTPILT
                     KRFFVWEQEAAIDAKVMRPNLIPYRNEFQEINFNVDVRNIDVHDAFDDIRVTVLQNGR
                     FDNASYNLKPRMVTNDMLMYDIDDLVFPGGKEYRRFDTKTLKFQTDRITKIQQQDGQY
                     HVYVNVDESRVYQRYTYEKDANGQYVIQAELSDDVNKEGDYAWIHFTLQYPYVITSGN
                     VFVIGEGMQGDTTLQAIPMTYNYDRQQYEAKAYLKQGYYNYQYALLPQGASYYALDYT
                     EGNTYESENDYVVLVYLHMFDKDYDRLIGCTVVNSVK"
     gene            24173..24952
                     /gene="mazG"
                     /locus_tag="PLM90_00085"
     CDS             24173..24952
                     /gene="mazG"
                     /locus_tag="PLM90_00085"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HMZ87789.1"
                     /translation="MQTSKEAKLEAFSRLLDIMDDLRSQCPWDKKQTIHSLRNLTIEE
                     TYELADAITGNDWKGIKEELGDILLHMIFYSKIGEEQEQFSIADVLHAQCEKLIHRHP
                     HIYGDVKVQDEDEVKRNWEQLKLKEGKRSVLQGVPNGLPALIKAYRIQDKAGQVNFEW
                     DHIDQVWVKVREEMQELQEAKDPDHVEQEFGDLMFALINLSRYLKIDPENALERTNKK
                     FISRFSYIEEQAAGMQKNLRDMSLAEMDALWDKAKAKEREA"
     gene            complement(24965..25669)
                     /locus_tag="PLM90_00090"
     CDS             complement(24965..25669)
                     /locus_tag="PLM90_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HMZ87790.1"
                     /translation="MSWLRNIFRKKLPAKGILLYGLQRSGTNYMETLLQLNYPDGLLL
                     NGTQRNEITHKHFRLYKEKEIIPEPQFANTLLVPTLKDFEAALPFQAPDLYIIVSKDP
                     YSWLGSYNKWSIKNNWPAHQHHYIMEYVLFYKMWMNYSREAKNILFVRYDDLLTDPAM
                     VMKQIAQVVEWPELEKVQSTNKVYASRRFTIEKKIAHLSGDQIKKINPQELRQINEML
                     PENLMEFMGYKKLMVN"
     gene            complement(25743..28673)
                     /locus_tag="PLM90_00095"
     CDS             complement(25743..28673)
                     /locus_tag="PLM90_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ87791.1"
                     /translation="MRNVCTLLIMFIMLLGAQAFGQNRTVTGVVKDAATGETIPGASV
                     MTKEDRSLGTATDINGEFKLEVPANTQALMVRSVGYAEIEVALTTSAKYEVMLTSEDY
                     NLDAIVVSASRRQEKILDAPASITLISPEQIKNTVAITPIQNIKGVPGVDIINTGLVQ
                     TNVVVRGFNNIFSGALLTMVDNRYAAVPSLRANVATFIPTDNLDLERIEVLRGPASAL
                     YGPNCSNGVFHMITKSPLSQEKKYMTSASLGLGFRSMISDTIMIANPIDPATGLVYGD
                     YTPLFDYDNIGERTFYTAGFRHSGKVNDQFGYRILFTYLNGMDWLYDDPFEPAQVQLG
                     YQTSEGRQNVGDTIDNTRDSHIEKIGVDLRFDYRLKNKAEFVLSGGMTQNDGIELTGI
                     GAGQAVGWKYYYAQLRYIHNDWFVQGFVNGSDAGDTYLFRTGDLIIDHSKQYVGQIQH
                     ANDWMDKKLNLVYGLDAIMTNPDTENTINGENEDDDNIIEIGAYAQGDYMVNPKWSLV
                     GALRYDYHNFVNNPFLSPRAALVYKANNKNTIRLTYNRSFSAPSSNNLNLDILQLGDL
                     TGYAGLFQGYLASSYVPNMPLYAIGNRDGFHYSYDDFGLPQYTSPYSAYLGYASDHYF
                     SLDNSYELNNVIWDLTLGLLEDAFDATIPGLGAALVAWLPPEIPVTSNNIAQLNLTTE
                     EFETIDPTNITDYSSMLNSATNTIEVGWKGSMFNDKVFATVDVYYNMMQDYVSPLTDI
                     TPNVFIDGAELAAYVAPIITENYYDTTGSNEFLASLVTATLDDDPAYGGNNNGTGVDE
                     FINLVLAAGAGLPVGTISPMEYPDGAKYVTYVNLGDIAVLGTDIGVSWYVNDDLTMQF
                     SYSWVDKDSVELEGAQLGYVALNAPKNKVGVRGSYDIKKADLNVGLTWRWQSSYPANS
                     GAYVGTVEEIHDMDLTLNYTPDFIKDTQITMLITNLYNHEQQYFVGAPKIGSTYMMKV
                     TKTF"
     gene            28829..32518
                     /locus_tag="PLM90_00100"
     CDS             28829..32518
                     /locus_tag="PLM90_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ87792.1"
                     /translation="MKIRRFHIILPIVFALCAITAYVIAGRAERSQSLEATAKHIEHD
                     YQTLEREIIAVISDEMIISNLITDSYTFEEFSRLAERPYGIVVYQNDSALIWTNNFIT
                     PIKAQVPFNDAPLLISESNGEYIIFQHDFPGDVHVIGFLPLQFEYGIRNKYLDHVKSP
                     GMHIPDFVRINPRAEGASTPVMSNSGNAVFYVSNDGLETAATVYPQSMFIMLILSALF
                     WLMCMYQLARWIAFKFGRLPAVGALLILLSLSYILLRDLFYDNVLSALPLFDPQFYGS
                     PGIAGSTGDLLIKTVWLFCFALFFSRFFFIKQLRSVWLKATLAIIVVLCTLVMGSIFR
                     SLISDSSISFDISNFFSLNYLSFIGFLCISVLLLSYFLIIIRCIELVIRSMHRPLEFT
                     VWVAVISILALLGEYFIGHALQTDMVCMITGFVLLRAVLMTKKINTRSLAGMMIWLVF
                     FSAFGAYIFHTGITKKEEETKRLFALKKSIEKDPITEYLFAGAETEIRHQLEMQETAV
                     EQGSRLRPGVIGQVRQNLQSNYFKKYDTDIFLFENDSLLITTDQQNPASRIGFIDAIE
                     NNGEFTAAENLYFINDYTGNYYYLAELTTPIDSNKSLTTYLRMVPRKFSGASIYPELL
                     IDDDLRTAESFIDFDYAIYNDHSLTEREGNYSYPLMDIFYINKDEESADIMENDYDHY
                     IYKVNDNKKVIVSTPAISWFQPVSLFSYLFFFFLVFLVIVLVLDWGAGLLQGKIRLKE
                     WIDTTLQNKIQSAVLSLIIFAFIMMGVATVFYISNQYNDSHKQRLLEKIEGVQTNIDF
                     LLGENKRKTAGAPVNTQLKKIGNRVTELSEIHAMDINIYKPSGALLASSQPDIFDKGL
                     VSRNMNPLAFFKMSCDKETWYIQTERIGDLSFLSAYVPVLDSEGETMFFLNLPYFATE
                     QNLKAEISSFMVTLVNVYVLLLIVAGIIAFFVSRSITKPLAAIAGKFREIKLGKSNEP
                     IFWTHDDEIGILVSEYNKMLKELDNSAQLLARSERESAWRDMAKQVAHEIKNPLTPMR
                     LSIQHLQRAIQTNAPNVQELTRKVTVTILEQIDNLTHIASEFSNFAVMPKPVNEQICL
                     NDVLDSVTALFNETENAAITLEMPDERIMIFADKNQVIRVFNNVIKNAIQAIPDDREG
                     IIAVSLESNEHRALVKVKDNGRGIGDEEKDKVFVPNFTTKSSGMGIGLAMTKNIVEGA
                     GGYIWFESTVSVGTTFYIEFPLMTV"
     gene            32518..33816
                     /gene="hemL"
                     /locus_tag="PLM90_00105"
     CDS             32518..33816
                     /gene="hemL"
                     /locus_tag="PLM90_00105"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HMZ87793.1"
                     /translation="MIARDKSTALFEEAKTLFPGGVSSPVRAFRSVEGAPLFMQRGEG
                     ALVWDEDGNQFIDYCCSWGPLILGHADKRVTQAVQEALVKGTSFGTPTRYENIMGQLI
                     INNHRFIEKLRFVSSGTEAVMSAIRLARGYTGRNKIIKFEGCYHGHSDALLVKAGSGL
                     VTLGETSSKGVPAAFANETIVVELDDEAAISSAIEQYPNDIACIIIEPVPANNGLLLQ
                     RKEYLQFLRNICDKHNIILLFDEVISGFRLGFEGASGLYNIQPDLITFGKIIGGGLPV
                     GAYGGKKEIMGMVSPDGPVYQAGTLSGNPLSISAGIAQLQACLTSGFYEELHRKCRII
                     ADKVNRFTAEAKLPFNILTTGSIFWLNFTEDCVIRKASDIKSTMPALFKPFHRYLLEH
                     GIYIGPSGYEVCFVSAAHTDEQLEQTADIIMQAITHTFQK"
     gene            33891..34820
                     /locus_tag="PLM90_00110"
     CDS             33891..34820
                     /locus_tag="PLM90_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ87794.1"
                     /translation="MWWSYLLHRNNLDNFNDVVAREKFVFTQERGPFLKFEDTANYKR
                     IYDDFKRTELMLYGEGLVFLFLIGLGFWRIRKTFREEIAMTRQQNNFLLSITHELKSP
                     LASLKLSMQTVQKRQLDAEHIRHLSGMALDDIARLEALVENILLASKIESANFQLNRE
                     LLDVSSLVTNILAHIAEKHKSQRQFTSDIEKDAYCYGDRLSFSSVVYNLIENAINYSG
                     ADGRIHVQLTGDEEHIYLQISDNGIGVPDAEKTKIFNRFYRIGAEETRTTKGTGLGLF
                     IVKHVVELHGGKIEVTDNDPKGTNFKVTIPVGA"
     gene            34896..35594
                     /locus_tag="PLM90_00115"
     CDS             34896..35594
                     /locus_tag="PLM90_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ87795.1"
                     /translation="MLNHRILVVEDEDHIRENLKLNLELEGYEVVTAADGAKAVKTFR
                     EQRFDLVILDVMLPEMDGFDVCEQIRLEDRETPILFLTAKDTSLDKIKGLKTGGDDYM
                     TKPFNLEELLLRIINLLKRSSKAPAEDKFNTYNFGGNSVNFVTYEANGINGNVKLTNK
                     ELKLLKLLIDRKNEVVSRTLILQTVWGYDVYPSTRTIDNFILAFRKYFESDPKNPEYF
                     HAIRGVGYKFTDKV"
     gene            35714..>38987
                     /locus_tag="PLM90_00120"
     CDS             35714..>38987
                     /locus_tag="PLM90_00120"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ87796.1"
                     /translation="MKRLLFLVVLCFCISTTAIFGQLVINEGSNRNGAVLIDEDGDYE
                     DWIEIYNNGLETDLYGYGISDDATLPLKWTLPNQTISANGFALLFASGKDRKPLLTYN
                     HWEQPISDTATWKYIIPSAATPSTWIQPAFDDAAWLSGNASVGYGDGDDATVVPDLTR
                     SVYLRYHFNVADVTLIEEGLLSMDFDDGFIAYLNGHVIAMYGFAAASPAYNAFSDSDH
                     EAAMYGGGVPEHFNISADSINAWLMTGGNVLAVEVHNVSAGSSDLTARPFLSLAVTVP
                     DIIWTDVLPEWFPIGGGTSFLHTNFTIAPSGEMIYLSDPAGLIIDSLFVGVADADQSN
                     GRITDGAADMQIFTTPTPNASNGGTSYPGYTLGDVTFTLDAGFYSGSQTIGITHPAGT
                     IARYTLNGEIPTSTSPIYLVPITIDTNTVFRVRLFDLLGELLPGRTFTNTYFIDENIT
                     TAVISISTNEDNLYGANGIFDNWWTDWKKLCYVEYFDTLGINQFEQQCGFKVDGGAGG
                     SRSLAQKSMRIEPDNPAFGDGTLNYPLIPRRWDTDEYETFYLRNGSNMSNVLPYKDAF
                     MVRTTEGTYNEHMAYTPVVVFINGAYWGVYELRNKLDEGHFDHVKGIEKDSLDLMTLS
                     YWYGLVLRTLSGSDTDFIEMRNYLGTYPTPADDDFYDIADSILDLNNFTDYIICQTWF
                     ANYDWPYNNIKAWRDRGGDNKWRYAIIDVELGLGIGGWSDANANLIPGLFYTQQYIEP
                     LATLLQNPKYREYFINRYADLMNSTYLTDRTLSMEDSIYNEIYPELPRQLERWGYGAV
                     EDQLATFEDYRNALRNDFSIRTNKVRLHIKDGFALENKVDVTLNATPAGCGHIQISTL
                     DIYDFPWTGVYFNGNEVQVTAVPLPGCTFDHWEANPFITDIYNPTFKVNIDAYTTTFT
                     AVFTGTPYEQKIAVSELNYHPESSLDDGNWIEIVNAGADPVSLDGWYIRDSEPYHRYD
                     IPAGIELNSGDRWVFADDTAKFHLLHPDVSNVSGPLGFGIGDYGEDMLLINARNEIVE
                     HMHYDDAAPWPLGADGHGRTLERNDMYADVNDPLNWFDGCIGGSPGLAYSPCEDPIVF
                     SEI"
     assembly_gap    38988..39087
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <39088..39894
                     /locus_tag="PLM90_00125"
     CDS             <39088..39894
                     /locus_tag="PLM90_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ87797.1"
                     /translation="SPGLAYSPCEDPIVFSEINYNSNAALDAGDWIELHNVTDEPLHI
                     GEWIFMDDSIGTEHQFMIPSGTTLEPHAHLVLAQDLALFNSAHPGVTNVIGGFPFTFS
                     GNGEWLRLYDQSEVLSFAVDYQDITPWPTVADGAGYTLELVDSLGVMNDGNNWTAYCL
                     GGSPGTFVIEPCPETIAINQISDIQSQISVYPNPVNQYALTAISIEYPVNASIRLFDA
                     QGGLRGTLFDGILQAGSFAISLDMSAYPAGMYFLQLQSDNKQSTIAVIKQ"
     gene            39959..40966
                     /gene="hemH"
                     /locus_tag="PLM90_00130"
     CDS             39959..40966
                     /gene="hemH"
                     /locus_tag="PLM90_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HMZ87798.1"
                     /translation="MMKKKGILLVNLGTPDSPATADVRSYLKQFLLDPRVIDIPAIPR
                     NLLVRGIIAPFRAPKSAALYKKIWTDEGSPLLVYSKKLHQKIATALGDNYSVVLAMRY
                     KNPSIESGLQLLANQGCTHITVVPLFPQYASATNGSVVEHVMAIVSKWQSIPDLHFTG
                     PFYDHPKFIQAIAQIASTYQLQDYDHFLFSYHGVPERQIHKAANGNACLAEGCCDVMH
                     KDNALCYRAQCFQTTRLIASALNLPTDKITTCFQSRLGKTPWIQPYASETITGLAAEG
                     KKKLLVFSPAFVADCLETIHEIGVEYDTEFRHLGGEKVQLIESLNDSDLFVECLVDMV
                     K"
     gene            complement(40970..43495)
                     /locus_tag="PLM90_00135"
     CDS             complement(40970..43495)
                     /locus_tag="PLM90_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HMZ87799.1"
                     /translation="MAYGTANRMVPKAEILTFAIEMMTRLFAVFAFLLMLTSVQQLEA
                     QIPGITISGTLLDSTNAKPLEYGTITIKHPGDSTIITGVLSDEAGKFKIQVATPGKYE
                     LQLYYVGYKRKILPLDITPGNPFTDLGKITLAADGGLLDVVEVTAERGYFQGSIDKKV
                     YNIEKDIIASNGSASDALQTIPSVVIDIDGNISLRGSEGVRIFIDGKPSGIAGSNMNA
                     ILEQIPANTIESIEVVTNPSARYDAEGNSGIINIVLKKNKKIGLNGNITAGVSTSPRY
                     EAGASLNYRNKKVNIYSNYSFTHDDRDGESYTYRKTFEEDTISYLLSNGSSNNISNMH
                     MARAGIDYYLTDHTTLGVSGSWHGNAGSNTGLVDYSFLNIDSTLTSTSKRTTSGTNNG
                     IGWNGGATIRHTFKDPKHVWSAEAYYASGSSDDSTYYAEDFYDATPAFWYSLVPQNIS
                     RPNENIDITAQSDYVRPFKNGNQFETGIKYTQEKKDNTFYSESFYSIGADPASWNADD
                     SLNNQFIYNEDVIAGYLIWNGTFKKKFGYQIGLRAEQTYTLSQLITTDEDFTHDYFGL
                     FPSAHVRYAVDETMEFSASYSRRIDRPNAWFLNPFRDYSDPYSFRQGNPFLDPEYENS
                     YEIGFEKEFKKHSLTASLYYTKTLNEISPYITVTPEGVSYMTFQNYNDESKYGFELVT
                     RDEFFKWWNMTGSFNFNQTLVNAENLEAGLTNNQFTYNIRLMHMFAVYKQTSLQLTMS
                     YNTPWTFAQGESNAIYFMDAGVKSDFFQNKLSVNVNFSDIFNSRKWSGVSEGTNFYSE
                     YSRKRQSQILSLKLTYKFGQQDNSRRRGNRGMDENYDGGVDMF"
     gene            43517..45148
                     /locus_tag="PLM90_00140"
     CDS             43517..45148
                     /locus_tag="PLM90_00140"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HMZ87800.1"
                     /translation="MFPKTIGDNGHYYRFVWLRFSIFRAETEMPVEDKIKRLHGMQAE
                     ALLGGGAERIASQHKKGKLTARERVHLLMDEGTFEEIGQLVVHRSTDFGMEKQQFYGD
                     GVVTGYGKVNGRLVYVYAQDFTVFGGSLSETHAEKICRIMDLAMQNGAPVIGLNDSGG
                     ARIQEGVNSLGGYADLFYRNTLASGVIPQISAIMGPCAGGAVYSPAITDFILMVERTS
                     YMFVTGPNVVKTVTHEEVSSEDLGGAVTHGSKSGVSHFTAANGLDCIQKIKRLLSYIP
                     QNCEEESPVYPYTMGDELRPALDRIMPENPNHPYDIREVITEVADVDSFFEVHETYAE
                     NIVVGFARVAGKSIGIVANQPAVLAGVLDIEASRKAARFVRFCDCFNIPLVVFEDVPG
                     FLPGTDQEWRGIITNGAKLLFAFSEATVPRITVITRKAYGGAYDVMNSKHIGADMNFA
                     WPSAEIAVMGPKGAAEIIFKKEIEDAADRDAKLQEKIDEYTETFANPYRAAFRGFVDE
                     VIKPSETRIRIIRSLDMLKNKVAVLPRKKHGNIPL"
     gene            45167..46258
                     /locus_tag="PLM90_00145"
     CDS             45167..46258
                     /locus_tag="PLM90_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HMZ87801.1"
                     /translation="MANSKRKTSLIGDKEIDFLRSYINNPSPTGHEAAGQKIWLNYIK
                     PFVDDHIVDNYGSVAAIINPTAPFRVVIEAHADEIGWAVQYITEDGFIYVGRIGGSDH
                     IIAPSKRVNIHTASGVVKGVFGWPAIHTRMGKEETPDMKNIFLDLGCTSKKDIEKMGV
                     RIGDPITFDDDFFVMNNYYVGRAIDNRIGGFMIAQVARMIKEQKVKLPFALYIVNSVQ
                     EEVGLRGAEMMAQTIKPHVAIVTDVTHDSNTPMISKTLEGDIKVGRGPVLTKGPSVHN
                     KLLQLMQDTADANKIPYQRDFASRSTGTDTDAFAYSNGGVPTSLIALPLRYMHTTVES
                     VHKDDVENIIKLMFYVLKSLKPNFNFKYL"
     gene            complement(46495..47631)
                     /locus_tag="PLM90_00150"
     CDS             complement(46495..47631)
                     /locus_tag="PLM90_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ87802.1"
                     /translation="MKKLSLILLPLLIGGLLNSCASQKSEAFEISGVIKGAENKKVLL
                     ESMTFPANGQPNFVVIDTAWTDNIGNFEIKNYLPERMICRMTIDGDQMNYYILSVHNE
                     KIKIDASWKDPGNPKVEGSSATNSLFGLMDALRSFDTEAGMMNDSLRTLKSLGKDSIV
                     DAILDDMKLNYFAIFKNYVDTTTVTSNAVLALESMYDFDFDYVKSYYNKAKNSADSSS
                     VYLKEMGEKITLREAELANSIVGKPFMDVEQPDPKGNMRKLSDLKGKVVLLDFWASWC
                     GPCRQENPNVVKAYNKYKSKGFTVFSVSLDDNKNKWLDAIKKDGLIWENHVCLLDQKN
                     NKAASDYRVTGIPMSFLIDQNGIVIAENLRGGALEKKLAELLPD"
     gene            complement(47690..49138)
                     /gene="gatB"
                     /locus_tag="PLM90_00155"
     CDS             complement(47690..49138)
                     /gene="gatB"
                     /locus_tag="PLM90_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HMZ87803.1"
                     /translation="MEVYDKYEAVIGLEVHAQLLTRTKAYSSDIAAYGGEPNSYVSPV
                     TLGHPGTLPVFNAQSLNLAMRLGLALHCQITRYNEFARKNYFYVDLPKGYQITQDKTP
                     ICTGGHVDIGAYGQERIIRLTRIHMEEDAGKSIHDLDPFYTLVDLNRAGTPLVEIVSE
                     PDLRSGDEAYAYLTEIRRLVRYLDICDGNMEEGSLRCDANVSVRLKGAAEFGVKVEVK
                     NMNSIRNVKRAIDYEIKRQIDAIEAGETIVQETRSFDAAKGSTFSMRSKEAAHDYRYF
                     PEPDLQPFIVSDAHIADVKANMPALPRDLMHKYVEQCKLSVYDAEILTAERDIALYFE
                     ELIALTNNFKAAANWMQGPVKSWLNEHAENIEALPIKPAQIAELIALTESGKLNFTIA
                     SQQLFPAMLENAEIAAEALAGKLNLIQQSDAGEIEKLVEEAISKYPEKVAEYRSGKKG
                     ILGLFMGEVMKLSKGKADPKLATRLLEEKLNA"
     gene            49458..51095
                     /locus_tag="PLM90_00160"
     CDS             49458..51095
                     /locus_tag="PLM90_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87804.1"
                     /translation="MRTFLPAVFLFFTITVSAQLQLPLQANRVSALFSANGFLFTDFS
                     YMAQYTVPGYSAGQENNATIFAGGFWMGGYDDADQLHLAASTYRQTGDDYWAGPIATN
                     YTNPDYIDRYSKVWPVDRAEIENHIANWDVPGYLVPPSIAYWPGNGNVANGEAAILAP
                     FYDYNNNLIYDPENGDYPLVRGDKAIYMIFNDAAEEHGESGGEALGVELHVMAYGFDM
                     PEDSALNQTVFVNVSVINRSVNNYNEFIFGNFADFDLGAYDDDYVGCDSNLNVFFAYN
                     GDPVDGPTFGNYGSNPPAQGVIFLNRPMHAFKYYNNDFSVTGNPTTGEDFYNYLNGKW
                     IDGTNQTEGGNGYGGAVPMNFMYPGHPMDPDAWTEMREENVPSDRRGVGSIRPVTLNA
                     GDSMCIEMAYLSAFVFDTVNAEGTTTDVHALGATEILLRRTVDVIDYYNANYEACQIA
                     YQSNPNYSSIVASDIANNISISPNPANDFIEVQLGGENVIGTIMLMDNQGRRIYESTL
                     SGQPSISLEITEYPAGMYNLLIIPESGAILSASFIKH"
     gene            51220..>55073
                     /locus_tag="PLM90_00165"
     CDS             51220..>55073
                     /locus_tag="PLM90_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87805.1"
                     /translation="MGKFRHLVWLMLLTSALHAQPGMLGTQAVNGSYTSYSLNDMGIF
                     RQMRVQATSSAASGTRNWEFYEAAADYDPAWRPYFSAMTLSGYNQPIAPSGASASATY
                     NLGFGGASGLLPAVTSGNYYTFNVTEKSTPGTPLDEHMGVMETSYNPVAITSVTQSPG
                     IGAVYPENSVYVTVTLASTLHADEYVYVRYSTTFNFTSSSLLPVTISGTTGTVEIPCQ
                     AASTTIYYYAYTSNRTSAEILADVAIPAIGEYAHDMSTLCINNNGGPNYSYTVLPSIG
                     FCGSYYIPSTCYPTINSFVTALNAGTVGCSVICYVAAGHTETAPAGGINLTQTGTAVK
                     TITFIKNGAGANPIIYAPVGTVSIVAASTVADGVFSLNGSDYITIDGIDIIDNNTTGA
                     TMMEYGYALFKASNTDGCQNNTIKNCAITLNNNNSWTGAPLNFEFGATGIMMRNTART
                     ALTTLFTITGVSGRSDNNQFYGNTITNVINGFVLSGYNDVTSPYTYYDQNNYVGVGGL
                     GNTIEKYGNTTATVRSSGVYAIYNNALVVSNNTIGNYLSGGTAHNNTLYGVFVSSPIS
                     GNNLQTLNIQNNTISLKGHTGSSRVTGIKVGNASCGATSITIDGNTIEDCTFTAGSTG
                     EFYGIDIGFNATAVAITNNIIQNNVINTTTTSVQYLIYNNTTGSAIDVSYNQMLNNYK
                     TVSSTAGILYGYYSASGSATGTQVFHDNIIDGLGIPSAQNGSVCAVRISSSINQSKEI
                     YNNAVSNITSGTSIATMYSCGYYIDGMNVGATVHDNSLSDVTCGNYFAGFNFCSAAST
                     ASTANVSYSCYNNTISNVTTTSTNTGPHGIAAYMPTTGSPVINIYNNNITGLNSTASF
                     SGYIYGIFMGGGSANATLNFYENTLHDFVHTGAATNTIVAGGLIALGGSTVNFYRNSI
                     NHITNTGSNGISYGLYITSTGVVNIYNNFIQRLSSTATTNVIGVYGIYTGSSGGFWQF
                     VNNTIALGYDTPLTSTGANFGFTGIYFAPTGVTATVLNNIVYVRGTANGTALGSCIRG
                     SIGTAYTPPASFSLSNNNYYFINNATYNYIYTEGGFNTSNVNGYAYGGAFTDVTRNLN
                     NDDCFNDPATGLYKAFMSPAESNSYYDVPPFAGGGSYPYNLKLTTGATTYAESNASAI
                     PGITIDYEGDARSGSTPDIGADEGDFVPQAVDCFLLPIELMSFSGEISGDDNLLIWTT
                     ATEHNTSYHIVERSVTGFDFTAIGSVPAAGESVSEITYNFVDQHPGSGIRYYRIRTVD
                     LDESNSLSNVIALDRELANSTQT"
BASE COUNT        15692 a        11168 c        11615 g        16498 t
ORIGIN      
        1 atacacagga tattcaacat ccttatgcaa cttccattct cgtagatggt gaaagaatta
       61 ttgccgttgg cgatgagact ttattgctgg aagcaggcga tattcaaaaa attgatattg
      121 gtggtcgaca tatttttccc ggattacacg atgcccatat tcatatatgg aaagtgggca
      181 atctgctgac taacatgctc gatttgcgcg gcgttggcag tattgatgaa atgcaacagc
      241 gactcagtga ttacgctcgc tcacatccgg aacttacatg gattcaagca cgcggcttta
      301 atgaagtaat atttcccgat aaacgcattc cgaataaact tgatattgat gcggtgattt
      361 ccgaccgacc ggtagtgatc acacgcacct gcgcgcatca ggttgttgca aactctaaag
      421 cgcttttgct ggcaggtctt gataaaaata tgcaacctgt tcccggaggc gaaataaaat
      481 atcttgcttc aggtgaatta agcggtcatt ttaccgaaac ggctatcggc ttgattttaa
      541 aggctgttcc aaaatatact gctgctgaat tgcgcaacat ggtattaaca gctcaggatg
      601 cttttctgaa gttgggaatt accgctgcct gtgatcctgc agtcgaccgc gatttgttag
      661 aggtatataa acaaatggat gccgatggcg aattgcgcat gagaataaat gccattccca
      721 ttcgcgtacc ggatggttct caaaaaatat atccgaaccc tcaacattat tattcatcac
      781 atttagttgt aaataccgta aagttttttg ctgatggcgg actgagcggg aaaacagcgg
      841 cactgcgcct accatacttg aatgatactt cgaaaggtgt aatgcgattg gaaaaagaac
      901 aattctatca attagcactt gaatcgcagg aagcaggatt taaaattgca actcatgcta
      961 ttggcgatgc tgctgttgat ttggtgcttc aagtgtatga taatttgcct ttgtcaaata
     1021 ttgaacacag gcttgaacat gttggatttg tggatgataa tcatctggac attatgtacc
     1081 gccggaaggt atcggctgtg atgcaaccaa tcttcattcg cgagctgggt aagaatttca
     1141 tagtatcact cgataaaaaa aggttggata gcatttaccc tattcatacc atagcagcac
     1201 atggcatcaa tgtggcatta tctactgatg cgcctgttgt tcgatccttt gatccgagaa
     1261 caaatatgct tgcagctcag acgcgagaaa cggaagaagg aatagtaata ggagcctctg
     1321 aaatccttga ccacaaacaa tctacagcta tgtatacaca tggctcatct gttgcggtag
     1381 gcaatgttgc aaacatcggc actattgcta aaggtcggta ttgcgatttg gttattcacg
     1441 actttccgga tctttcagga tcagcagaag tatggctcag tggttccaag attagatgaa
     1501 tttttaaagt ttatcatgca gatgagggtg atttttaaca cttaatagcc ccatttaccc
     1561 aaaaaaaacg ctatacttgt agcttaaatc tctaaatatg actattcgtt acgcattaac
     1621 ttctctcgga aagcctctga tggctgcttt ggcagttgca ggtggtttga ccgtatctgc
     1681 gcaatcgtac atacagagta cgtgtgatta cgcccctttg acaactgaag gaactgtgat
     1741 ctgtctgggt gatgacgcag ttagctctgc catccctctg ggattctcct tcaatttcta
     1801 tgatgttcca tacactgatt gctatgtgag ctcaaatgga tacctttcat ttgcagcagg
     1861 tctcggaagc gcatgttgta caggacaaat ccttccaaat gcaacttatc cggcatctat
     1921 tttcttcgga caggaagact tggatccaaa ctcttgtatc gatggagata tttcatatta
     1981 cactacaggt gctcccggtt cacaaatttt tgtgttgagc tttgtagatg ttccgcacta
     2041 tcccggtccg gaaggaacat tccctgtaac tgtacaagta caattgtatg aaggtacaaa
     2101 tgaagtacgc atcgtaacca ctcagtggaa ctccgatggt ggtgctgcta ctatgggttt
     2161 aaatgagaat ggttcaaatg ctgatccggt ttcaggaaga aacagtgaag tatggtctgc
     2221 attcgatgag tgtatttctt tcgtacctaa taccgaacct acaggtggat gtatggatgc
     2281 cgctgctatc aactacgatc cgggtgccga tttcgatgat ggtacttgtt tctatggtta
     2341 tatcgcttct acttgcgatt actcaccaat ggcaactgaa ggaacaatcg tatgtcttgg
     2401 cgatgatcaa gtgagcacag caattgatat gggcttcgat ttcccattct atggcacacc
     2461 acataatcaa gcatatatca gctctaatgg ttttgttaca ttccagcccg gtatgggaag
     2521 cggatgttgc acaggccaaa tattgccaaa tgctacttac caaaatacca tctttattgg
     2581 tcaggaagac ctcgatccta atacttgtgt agatggcgag atcaactatt atactacagg
     2641 tgctccgggt tcacaagtat tcgttgtgaa cttcatcggc gttcctcact atcccggacc
     2701 tgcaggtact ttcaatatca ctgttcaatt gcagtgctat gaagctacag gcgagattcg
     2761 cattgtaact acagaatgga atagcgatgg tggcgctgct accatgggtc ttaactacaa
     2821 tggcacttat gccgattggg tacctggtcg caactcctct gtttgggatg cattcgacga
     2881 atgttattct tggatgcctt tgacagaacc actgcctgtt tgtgatgctc cgggaggtct
     2941 ttttgccgac ggaattacta cagacgatgc tgtgttacat tgggacgcag ttgatggtgc
     3001 aatgaactat aatgtaattc tccaaaatac tgcgacagga ttattgaaaa agaaagtatt
     3061 cactaccaat atggctgtga ttgaaaataa actttctcca ctcactaact atgctttccg
     3121 cgtgaaaact ggttgcggtg tagatcctga aaatatgtca gatccttcac cttggtatta
     3181 tttcactact ctcggaagaa caggcgaaga acaagctacc gttactttgt tccctaaccc
     3241 gaataatggt gttttcactt tgaatgttgc cggttatgcc gacaatagtt tcaatttgaa
     3301 tatctataat acaatgggtg caattgttta tactaaaact gttgatatca cttctgataa
     3361 tcaagcagtg gttgtatcat tggaaaatgt tcctggtggt atgtatcaaa tcaacctgag
     3421 caatgatcaa taccagatca actatccggt gattgttact aaataattga tttaacttat
     3481 tattttgtaa aagggtgtcg atttcggcac ccttttttat ttgaaatgca acgtggttta
     3541 catgtagtac atcaaataat tgaacttgca actgcgagag agattgtaat aatttcaaca
     3601 attaatgaca tttattaatt atctgtcatt gcatagaggt catcagcaat ttgtacatga
     3661 taatctcttg tcattttcaa attctttcgt atattgcagt cattcttttt taacctaaat
     3721 cacaacttat gatgaacatg ctttcttacg caaaaacatg gcttactcta ggtgtggttg
     3781 tcgttactac cggattagcc agtgcacaat attgcacacc tgtctatact accggaactg
     3841 ctgatggcga ctatatcaat ggagtaacag ttggcgcgat tgccaacacg ggttccggtg
     3901 ccgcaattac cggagtgggt tattcagact acacggcatt atcttctgat cttaccgttg
     3961 gttcaaccta cacaatgacc cttgataaca acccttcatg gtctcaaacc tatacagcct
     4021 ggatcgatta tgatcaggat gaagtatttg aaaccacaga ggtattgggt ggtgtaggac
     4081 tttcagccgg tgcttccgga acagttacat ttactgtacc tgttggtact ccgggtggtt
     4141 caactcgcat gcgcgttcgt tgtatctacc caagcggtct tgcaattcct caggatcctt
     4201 gtttatctgc aacctatggc gaagctgaag attacaccat taacattgtt ggcggtgctg
     4261 ctaacgacgt tggtgtaatt gacatcacta acccaatttc agctgttgat cttggctttg
     4321 aaaacgttac catcactatc cgtaactacg gttctgaacc tgcttcagga tttaacgttt
     4381 acttccaggt agatggcggt ctggttacag gcgctactta cggcggaaca atcgatgcct
     4441 actcaactgc atcttacact tttggtgata cttgggattt tactgctgat ggttgctacg
     4501 acatcactgc atggaccgat tatgaactcg atgaaattgc agataatgat gactataccg
     4561 aaacagtttg taacctcggt cctattacag gaacaggtgc ttggtacatc tactctaact
     4621 ctacaggcgg tgagccttgg tttactacaa ccaactcaac tgctatgact acagtattcg
     4681 gcgctgaagg aacaggatgg aatcgcgctt tcttcgaaac actcgatcca ctgtcaatct
     4741 tcaactctga caactgcttc gtatttatcg atggttctga tgctcaagct aatgaacttg
     4801 aaactttcct cactgctaac atgtctacta tcgaaaactg ggtgtcttca ggtggtaaac
     4861 tgttgctcaa ctctgctcct aatgaaggtg atggcatgag ctttggcttt ggtggaacta
     4921 cacttactta ttcatactac acttcaaatg caactgctgt tgatgcattg catccaatct
     4981 tcaacggtcc tttcactcct gcaggaactg actatacagg tacttcattc ggtcacgcaa
     5041 tcgtgagcgg tgttgatctt agcaacgtaa tggttgatct cttcaatcca tctgcagttg
     5101 tattggcaga aaaaacttgg ggcgatggtc gcgtgatgtt tggtggtatg actactaaca
     5161 acttccattc accactcaca aacgctgcaa acctgcgcgc aaacattatc gcttatatga
     5221 gctgtatgga attcgttatc tgcttgcctc cgggaggttt atatgctgat ggcattacta
     5281 ctgacgatgc agtattgcat tgggatgttg ttgatggtgc cctcaactat aacgttatcc
     5341 ttcaaaatac cgctacaggt ttgttgaaaa agaaagtgtt cgcaaccaat atggctgtga
     5401 tagaaaataa actttctcca ctcactaact atgctttccg cgtgaaaact ggttgcggtg
     5461 tagatcctga tatcgtttca atggcttctg aatggtatta tttcactacc ctcggtcgcg
     5521 aaggtgtgaa tgaagccgca gtttctttgt atccgaaccc taacaacggt acgttcacta
     5581 tcaatgtagc aggttatgaa ggcaatagct ttaacctcaa cgttgttagc gctaccggtc
     5641 aaatcgttta cactaaaact gttgacgtaa caggtgctaa ctatgcagaa cagatcgctc
     5701 ttgataacat ccctgcaggt atgtatcagg tgaatctgag caacgctcaa taccaaatca
     5761 actacgctat cgtagtaact aaataattcg ggtttccgaa gttgaaaagg cggtcgattc
     5821 gaccgccttt ttttatgcca taaatgacca aacagttatt tattttttca atacaattat
     5881 catattgaac ttttatataa gtaaagtgaa tatgatatgt aaataattga aaatcagtta
     5941 attatgtgat agtttgtaat catttccaat gaaatgagca aaagcaaaat ttgcaacatg
     6001 atctaagtat gcaaaggcct gatttttaat cattattttc aatgattcat ttcacttaaa
     6061 cgactcatat gaaaaacatg cttaaacatt tacgaacagg tttagtactg gcagcagtaa
     6121 taggctcttc aatcgctgct aaagcgcagt attgtacgcc gacttacact acaggaacag
     6181 ccgaaggaga ttacatcaat ggcgtaacag ttggcactat cgccaacaca ttttcaggtg
     6241 ctgcaataac aggtgttggt tattccgact atactttcct gtctacagac ctgaatatcg
     6301 gcgatgctta caccatgaca tgtaataaca acccttcatg gtcgcagact tacactgcct
     6361 ggatcgacta tgatcatgac aacacgtttg aaacatctga agttttgggt aacctgagtt
     6421 tgagtgcatc agctacagga tcattttcat ttacggtacc tgtaacagca atgtccggaa
     6481 ctacaagaat gcgcgtaaga tgcatttatc cttcaggttt agttacacct cttgatcctt
     6541 gtgccttagc tacatacggt gaaacagaag attatactgt taaccttttg ggtggcgcta
     6601 tgaatgatgt tggtgtaatt gatatcacca acccaacaac cggtcccgga ttaggaatgg
     6661 aagatgttat cgttaccgtt cgcaattatg gttcagaacc tgcttcagga ttcaccgtat
     6721 cttatcaagt tgacggcggt ttggtaacag ccggatttta tggtggtacc attgcacctt
     6781 tttctaccgg ttcatggact gcacctgaag gctgggattt cagcgcagaa ggatgttata
     6841 atatcactgc atggactaca ttagcaggtg atgaagatcc gggcaacgat agctataccg
     6901 aagaagtttg tcataccggt gccgttaccg gaacaggagc gatctatatg ctttcaaaca
     6961 ccaccggcgg tgaaccttgg tttactacca ataatagtac tgcaatgacc accgtttttg
     7021 gcgctgaagg taccggatgg aataaaaatt attatgaaac tgctcctgca gctactgtgt
     7081 tcacccctga taattgcttt gtatttatgg aaggttctga tggtcatgcc aatgaacttg
     7141 aaacatatct cactgcaaat atggcagtta ttgaatcttg ggttagctca ggcggtaaat
     7201 tattattaaa cgctgctcca aatgaaggcg atggcatgag cttcggcttt ggtggaactt
     7261 cattggttta ttcttatttc acaagcactg caaacggtgt tggcggtcac ccgattttct
     7321 ctggtccata tacacctgca ggaactacct ataccggaac ttcattcgga catgcacgca
     7381 ttacaggaac aggtcttacc aatgttatgg ttgatgctgc tgctccttca aatgttgttc
     7441 tcgctgaaaa aacttggggc ggtggtattg taatgtttgg tggtatgact accgacaact
     7501 ggcacgatcc tgatcctaat gcagcaaatc ttcgcgctaa tatcttcagc tacctgtctt
     7561 gcgaagtggt aactgattgt cctgctccag tgggtcttgg tgctgatgca attacaggta
     7621 cttctgcaca cttgttctgg gatccatcac tgaactccgg tggttatcat ttgtcagtgt
     7681 ataaaactga tgaaacattg gtgttgaaga aaaaagtact cggtggtgct acagattgga
     7741 ctgtaacagg tcttactccg ggaactgatt atgcattcca cgtgcgttca atttgtaccg
     7801 gtttaggtat ggcttctgat atttcttatc actactattt cactactcct gctcgtcttg
     7861 gagatgaaaa tactaatgta gtgttattcc ctaaccctaa taccggaact tttgatattg
     7921 cattgaacgg ttatgaaggt caacaagtaa atattcaaat tgttagcgct ctcggtcaaa
     7981 cagtatataa caatacggtt aatgtgaatg ctacaggatt tactcaaaat gtaaacctca
     8041 ataatgccgc tgccggaatg tattacgtaa atatcttaac tgataaaggc gtaatgaatt
     8101 atcaggtagt gattaccgac taatcacctc attagttctt cataagaaag gctgtccgtt
     8161 tgggcagcct ttttttatgg tatttgtaaa tacttatgcg tctgcaaaga aatacgccat
     8221 tgaggatttg tgcgcacata ctcagtgatc aatggcatca tactatctcg cttttcccat
     8281 tccggttgaa gatataatag acaagattca cttacggtag ccgcatgcat ctcggcaaat
     8341 acaagatcgc ttttatgatt gatcacaacc ttgagttcat tggcttgtgc agctactgat
     8401 gctaagggcg atttaaattt cttcggagaa aatgtaatcc aatcccaaat gcctgataag
     8461 ggatgggcac cacttgtttc taaatgggta tttaaaccct tttcatgcaa ctcatttgtc
     8521 aatgctgata aattatgcat caagggttca cctccggtga ttacaacaat tttagatgcg
     8581 aatgctgcgg cattatttac cagcgatgaa acactcagtt ttggatgagc atcaacatcc
     8641 caactttctt taacatcaca ccagacacaa cctacatcac aacctcccaa acgaatgaaa
     8701 taggcagctc gtccctgatg aaatccttca ccctgaattg tataaaactg ctccattacc
     8761 ggtaattcca tgtgctgagg aaattattga tgaaacaaaa ataattatag tttatcacgc
     8821 gattctgctg cctgcatggt attcatgatt aatgatacca ccgtcattaa accaacacct
     8881 ccgggaacag gtgatatcgc agaacatttt ggcgctacat tcggaaaatc aacatcacca
     8941 agaatgagat atcctttttc attgtcggca tccacgcgac tgataccaat gtcaattaca
     9001 acagcacctt cttttaccat atcgcctgta acaaacttcg gtttgccgag ggcagcaata
     9061 atgatatcgg cactgcgaca aatttccgta agattttttg tgcgactgtg acataaggtt
     9121 accgtgcaat ttcccggatt ggtatttcgc gataataata ggctcatagg tgtgccaaca
     9181 ttattgcttc tacctatcac aacgcaatgt ttgcctgcag tatcgatatg atatctgctt
     9241 aacatggtca taatgcctgc cggtgttgcc gaaataaatg caggcaaacc caattgcatt
     9301 ctcccgagat tcaccggatg gaaaccatca acatctttcg atgggtcaat ggcttcaaca
     9361 atatgcatct cctgaatatg ttttggcaat ggcaattgta cgataaaccc atctatatcg
     9421 ggatttgtat tcaaatcatg tacaacacgt agtaattctt cttcagaagt agaagcatct
     9481 aaatgaataa gcgtgctttg catgcccacc gatttacaca atcggatttt gctgtttaca
     9541 taggtagtac tcgccccatc attaccaaca agcacggctg cgagatgtgg aatttttaat
     9601 cctgcagtac ggcgtatatc gatctttttt ctgatctcat cctgaatctc agtcgataat
     9661 ttctttccgt ctaaaataat tggctgtgcc atccggtatt gcagaattta ttgtttattt
     9721 gaatacagaa atgcaccaat gcactatctg taatttaatt gtatgcgatc gcagtgacga
     9781 ttaaacaaga atatcactca ttcagtttca atactgcaag gaaagcttcc tgcggtactt
     9841 ctaccgtgcc tatctgacgc atgcgttttt tacctttctt ctgtttctcc aacaacttgc
     9901 gcttacgtga aatatcacca ccataacatt tagcggtaac atcttttcgc atggcactaa
     9961 tggtttcacg tgctatgatc ttcgcaccga tggcagcttg aatggcaatt acgaattgtt
    10021 gcttaggcaa gagatctttc aatttaccac acagcctgct gccaaaatca taagccttat
    10081 cgcgatggat gagtgccgag aaagcatcta cctgctcgcc atttaattta atatcaagtt
    10141 taattaaatc gccctcacga taatcaatca attcgtagtc aaaactggca taaccgcgcg
    10201 aaatggattt taatttatcg taaaaatcaa atacaatttc cgctaaaggc atctcccatg
    10261 ttagctcaac gcgattggta gtcagatata cctgattttt caatataccg cgcttgtcca
    10321 tacacaattt cataatagca ccgatataat caggcgctgt aatgatctgt gaacgaatat
    10381 aaggctcttc aattttatca atacttcccg gatcaggcat gtcggtcggg ttgtgtacca
    10441 cgatcttttc gcccgcagtt gtataacaat cgaaagaaac gtttggaacg gtaataatta
    10501 cattctggtc aaactcgcgc tctaatcgtt cctggacaat ttcaagatgt aacagaccta
    10561 aaaatccgca acggaatcca aatcccaatg ctactgaact ttcgggttca aaaacaagcg
    10621 atgcatcatt cagttgtaat ttttccaatg agtcgcgcaa atcggcaaaa tcgtcgttgt
    10681 cgatagggta gatacctgca aacaccatcg gtttcacatc tttaaatcct gccacttcat
    10741 tgctgcaagg gttatcacga agtgtgatag tatcacctac cttaatttct ttcgcctcct
    10801 taattcccgt aatcacatag ccaacatcgc ccgccttaac ttcggaacgt ggttcatatt
    10861 gtaatcggag aattcctact tcatttgctt cgtaattatt ttgggtgttg acgaatttga
    10921 ttttatcgcc ctttttaatt gttccctgaa aaatccgata gtaggcaaca acaccccgat
    10981 aactattaaa cacgctatcg aaaatcagcg cttgtaatgg agcatccaca ttgccttttg
    11041 gcgaaggaat gcgatgtatg atggcatcaa aaatgctatc gatacctacg ccggttttgc
    11101 cgctggcttc aagaatttct tcgcgtttac aaccgataag gtcgacaatc tgatctttta
    11161 cttcatccac catggcacca tccatatcga tcttattcac aaccggaata atttccagat
    11221 cgtgttcaat tgccaggtaa aggtttgaaa tggtttgcgc ttggatacct tgagtagcat
    11281 caacaagcaa caaggcgcct tcacatgccg caatgcttcg cgatacttca tagctgaaat
    11341 ctacgtgtcc gggagtgtcg atcagattga gaacgtattg tttcccctca tatcggaaat
    11401 ccatctggat ggcgtggctt ttgatggtaa ttccgcgctc ccgctcaata tccatgtcat
    11461 cgagcacctg agcctgtgct tcccgctctg aaacggtctt agttacctcc aataggcggt
    11521 cagccagggt gcttttaccg tggtcgatat gggcaatgat gcaaaagttt ctgatatgtt
    11581 ccatccttca aatccttcag ggaggtgcaa agttacgaaa ttgccgctcc aacacgtagc
    11641 aaacctgaag ttagacacgt tgataagtgt taacggcttg gcgattatat gttatctttg
    11701 cagcgcaaaa agcgacctac aatgacacac aactatcaaa ctgcatcccc tgttaaacgt
    11761 atttcggcat tcgctatcga taccatactt gttggtgttg tttctgctgc ctttctgttt
    11821 gttactatgg gcaaaacccc tgccaaattg caggcttcgg gtgacgaagg gcgtccaagt
    11881 attgccgata agtaccaatt ctttgcttat gcagagccta tcgtcaatga cccaaacagg
    11941 gccatttata tcgagaattt tttgaaaaag tataagatgc aggcaatggt aggtctgttt
    12001 ttactgccat tactgtatta tgtactgttt gaaggccttt ggggggctac catcggtaaa
    12061 ttgttgaccg gcatccgtgt ccgccgtaaa gatggtggca aaatcagtat tggaactgcg
    12121 tttttgcgcc atatcggaag aatcatatcg accatgattt tcatgttggg atacttgctt
    12181 gcatttatcg acggaaaacg tcagactttg cacgataaga ttgcaaatac aatggtgctg
    12241 aagaaaggaa caggcatctg atcaccattt aatccttaac tgcagtttcg catcactgcg
    12301 ggtatttccc ggaaacgtat tatcgccgga acctaaaggt tcgtcccctg aataccaggt
    12361 gttggctact cttgcccata catccatcca cttgaatggc gaccaggtga acatcagata
    12421 tgtcctggtg ccgcgaccgc ttaatccaac gatggaatat gcatataaca cttccgtttc
    12481 atatgcataa atacgggtgt tgtaagaatc ggtattaaaa atcgcaaatc gcgtagcaaa
    12541 acctataggg ctgctcaatg gtttataatt gaaatcctgg aataccaaat atccccgctc
    12601 aggaatgcca taggtctcct cgtagaaact cacctctatg cgcgatttta ccgtaattgc
    12661 tttgtttaca tcataatcgg catgccacct tatagaactg cgcattaaat cgctcacaac
    12721 atttaattga ttttctgcgt caataatatc atcggtgctg tttctgtcgc tattttctct
    12781 cttatagcgc acataagtgt tgaaacttcg cgatggttgc catgttaatt gagcaagcaa
    12841 atctgtgcca taaccgggag cgtcggtttg atattcgagc cattggtgtt tatacaaatc
    12901 tacataccct gaaagtttcc atccctttat tggttttatt tctgtgccga agtaagtacc
    12961 attttcattt tgaggcaagg tagcatcaga aaaagttgca gcatataaag tttgataatc
    13021 gggagaaaaa tatcgatgca gtatggcgat atccattttc ggatcaagcg gaattattac
    13081 cccattgagc gttcctgctt ttccattatc actcatggct gtttcaccaa agagcagcat
    13141 gtttcgccag agaaaattat aatgcgtgcc gaagttgatc aattgctgac cggtgaaatt
    13201 atatatttca taaggtgaaa ttgttggttg caacggagca gaataatgat aaccgatagc
    13261 agaaaatcct atgttcacct gatccttata ccaggtaaga tcaaatccgc ccattaattc
    13321 cttcaatgca tccttgcgtt cgagttcact ttctgtgcga tgtaatccac tctcatcaat
    13381 tgccgacact tgtattactt cgtcacttac tgtatccaat aatgcaacat ttgcatcaat
    13441 tgatttatat gaaataaatg gcgtgaattg ccagcgaccg taagcaagtg tagctcccgc
    13501 tccgcgcaag aaacggtttt catttgttga ggtgtaactg tcgagcaccg gtccggcgcg
    13561 tctgattgcc aaagggaaga cgctttttcc aaaaccaaaa ccgctccaca tcattaatcc
    13621 ctgaccgatg cgtaattcaa aatcacccaa agcaagtgcg cgcatggtgc ctgtgccctt
    13681 tttgaaaaag tgggcagaat aataatcgaa gccataaggt tgtgtgggcc cgaatactgc
    13741 ttctccggca tctttctctg ccgtgaaacc atatgataat ttattgttgt agttgtacct
    13801 gaaacgattg tatacacgta aattatttcc tgcataggag gtattgcctg cactgtctgc
    13861 aacatatcca tcctgagttt ccagatagcg gcttaacctg gtataatatt gataatcgcc
    13921 aagatataat tgatcaataa atgcaacctt gtctctgcta acctgattca atgtaataaa
    13981 cggcagaatt cgtgcaatgt cttccggata aaaagaaggt atcgtttgca gctcgtataa
    14041 atttaataac gggccgtaag attcaatatg tcggcgtaaa tctcgtatct gcacttcagt
    14101 aaataatccc gaattgcgta attcgatata agatgctgta ttaatcgaaa ttggattttg
    14161 aagtaagtag ttgagatttt cctgataggt gtcgatagac aaatcggcat tatctgtttt
    14221 ctcggcggcg ccttcaataa tgtcgtcttc gagtaattta ccctcaccct tgttttcctg
    14281 tgcatgggca aaagaaaaca tcatcataaa agaagtgcaa atgaagatat gaggatgaaa
    14341 tattttcatg gattattatt catcaaaatg catactgtaa agacacaccc ggtgtaacgc
    14401 cgagttgctg atggaactga gcaccggcat ctaacatgaa agtttttaat ttcaacccaa
    14461 ttcccgtggt gaacatagat gggttggaga tgtaaccacc tctgagatat aatacatcga
    14521 taatgtggta ttcaattcct gtttttatac gcagatcctg atcaatattt tgttctcctt
    14581 ctaaacacac ccgtacaaat tctgagggtt gccattgcaa tcctgccgta ataattgcag
    14641 gaagttcctc agcatattca agtccgtttt tcactcttac aggattaaag gctttagcgc
    14701 cgatagataa agttttagtt ggtttgtaca acacgccgat atcaaatgta aatgcagttc
    14761 cgttacctac ctcagatgtg ttagtgccaa cataatctaa ttgcactcca atagctaaca
    14821 catcagccag cttgcgacca taacccaaac ctatttttgt ttgattaaat gttgcatcac
    14881 cggtataatg agcggtaatg ccaaaatttc ctgctcgttg aaccggaata attcctgcag
    14941 cataaaatgc attcaatcct tcatccatga ggaaataatt ctgtgtgctg atgcctactg
    15001 aagcattctc tgcaaaggcc attgctgcct gattgttttg ggcgctgaat acatcggggt
    15061 tagcaatacc tgtattcccc atactattgg ccgaggctcc cgcatcagag tagccggttt
    15121 gggcatgcac agataagcta atgcacagaa tagcacatgt gagtaatttt cccgggttca
    15181 tacaggtaaa gatataagaa atcctgcaag acatttcagg ctatcaacat tccgaaaatg
    15241 ggcatttggc tgactatgat tattttagca tcttcaataa acctataaaa ccactgagat
    15301 atggatgtta atatgatttt aattgcgatt ccggtgctcg ggctcatcgc attgttattt
    15361 acctacatga agagcaactg ggtagcacgt caagatcccg gagatgctaa aatgcaaggt
    15421 atagccggtg caatcgctga tggtgccatg gctttcctga gagctgaata tcgcattctg
    15481 gcaattttcg ttgtcattgt tgctatccta ttagggcttt cgggtgcaag tgaagaaaat
    15541 tcttcagcac tggttggtgt atcttttgtt gttggtgctt catgctcagc attggccggt
    15601 tttatcggta tgcgcgtggc tacaaaagca aatgtgcgca ctgcaaatgc tgcaagaggg
    15661 ggacttggaa aagcccttga aattgccttc agcggtggat cagttatggg tatgggtgtt
    15721 gtaggtctgg gaattcttgg cttgggaacc ttattgctca tctattcaaa tatttatggg
    15781 ttcgattctc aggaagcagc aatcaaggtg atcaatgtag ttaccggatt ctcattcgga
    15841 gcatcatcga ttgcactttt tgcccgtgta ggtggtggaa tctataccaa agctgccgat
    15901 gtgggtgccg accttgttgg aaaggttgaa gccggtattc cggaagatca cccactgaat
    15961 cctgctacca ttgcagataa cgttggtgat aatgttggcg acgttgccgg tatgggtgcc
    16021 gacctatttg aaagttatgt aggttctatt gtcggaacta tggttttggg aacagcattt
    16081 atggcaggaa ctattggtgt tggcacttca tttatggaag atggatttaa cggtttgtct
    16141 gctgtattac ttccgcttgt tttaggtggt gttggtatcc ttacttctat tgtaggtact
    16201 ttctttgttc gtgtgaaaga aggtggtaat cctcaaaaag cacttaatgg tggtgcacta
    16261 ggttcatcaa tcgcaatggt gattttatca tactttatca tcacatggat gttgcctgaa
    16321 aaatttacgt ttactgatgt gctttatgga ggtaaagaac gaacagtaac agctataggt
    16381 atttttattg caacagttgt aggtctcatc gcaggttctg caattggatt tattactgaa
    16441 tattataccg gtacaggaaa aaaacctgtt atgaaaattg ttcagcaatc atctacaggt
    16501 tctgcaacaa atatcattgc cggattaagt gtaggtatga tgtcaactgc cattcctaca
    16561 ttaattattt ctgcagctat cattgtttct tttatgttcg gcggattata tggcatcgcc
    16621 attgccgctg taggtatgct tgctaatacc ggtattcaat tagcagtgga tgcctacgga
    16681 ccgatttctg ataatgcagg tggtattgct caaatggctg atcttcctaa agaagtgcgc
    16741 ggcagaacag ataaattaga tgctgtagga aatacaacag cagctatcgg aaaaggattt
    16801 gccatcggct ctgcagcact tactgcgctg gcattatttg gtgcatttat gcaagtaacg
    16861 catctgactc aaattgatat ttcgcaagca cacgtgatcg ccggtttatt tatcggggcg
    16921 atgttgcctt tactattttc agcaatggcc atgcaaagtg ttggtaaagc tgccatgagc
    16981 atgattcagg aggtgcgccg acaattcaac gaaattccgg ctttaaaggc tgctcttgat
    17041 gccatgaaac gcaatggcga taaagaagta catgagtggt ctaaagcaga tcaggaaata
    17101 ttcgaagcgg caaacggaaa agcagaatat gcaaaatgtg tggagatttc cactaaagct
    17161 gccgtaaaag aaatggtggt tcccggatta atggccgtta ttgttcccgt aatcgtaggc
    17221 tttgctccga aagtacttgg actcaatacc gaaggtcacg aactaatggg tcctgaaatg
    17281 ttgggtggct tgcttgcagg agtaactgta agcggtgtgc tgatggcaat ttttcaagcc
    17341 aacgcaggtg gagcctggga taatgctaaa aagatgtttg aagaaggggt tgatattaac
    17401 ggaaaaacct actacaaagg cagtgatcca cataaggctg ccgttgttgg cgataccgtt
    17461 ggtgatccat ttaaagatac ttccgggcca tctatcaata tcctcattaa attaatgagc
    17521 gttgtgagct tggtgcttgc gtctttgatg gtgtaagcta attttatcaa tacttgaata
    17581 gccctgattc cacgagtcag ggcttttttt tgcacggttt ttgtaaatta gaggtatgag
    17641 aaggattttc ccatgggtat tggtatgtct gtaccctttt gcaatgcatg cccaaattga
    17701 taccgtcaca ctcacggaag atggtcattt ttatttcgaa aattttcttc cctattcggc
    17761 atattcgtca ctgatgaata gaaatggtga accatattta tatgcagctt gtcgcgaact
    17821 tggtgtggtg accttcgata tttcggatcc tgattttccg gtgcctgttg ataccattcc
    17881 cactaccgca ttaaataatc tcattcctaa taatatttcg caaaccggcg attatttata
    17941 cgttgctaca ggtggcttta atctcatccc tcaaaaggca ggactagcca ttatgaatct
    18001 tgctgatgca gcacatcctg ttattaccga caaatgggat tcagtagctt ataatcaggg
    18061 cgctgccatt gccattagcg atggtaatta cgcatacctt ggaggtatgg acgatggatt
    18121 gataattctg gatgtgagca ataaatcaaa tatcacattc aaatcatcca ttctccctga
    18181 tccaaattat cctgaagtgc cggatttgtt ttctgttccg aatgttcgtg gattagcact
    18241 ttatggcact gatacactca tggttgctta tgatgcgggt ggattgagaa tgttagacgt
    18301 gagcaataaa tcagcaccaa ttgaaatcgg aaaatatgcc gatccggata tagaaaatat
    18361 tgcacagtcg gcgtataaca atattgccat caaacaacat tatgcctaca taccggttga
    18421 catgtgtggt ttacttgtag tagatatagc agatagtgat atgagtacag tggcctggtt
    18481 taatccctgg gattgcgatt ctacaaactg gaatggcagg ccgggacata caaatgaagt
    18541 gcggataaat gaggatgaag acttattatt tgtaagtggt ggcgacagtg aattattgat
    18601 ttttgatatc tcaaatccgg caacgcctac actaaaagcc acttatggcg tgccctttga
    18661 tagtattgtt gcctggagtc tggatgtatt tgggaatcaa atttcacttg cattagtcga
    18721 taattcgttt gtaggctttc cttattattc tgatgtaggt ggcattcaat tattgcaata
    18781 tgaagcaatt catactattg atattcaatc gttgtataaa gaatttaagg tgtacccaaa
    18841 tccttctacg ggagtcattg ttatcgatga tattccatca ggcagcgcaa tgagaataat
    18901 gaatctgcag ggtgtaatat gcgagcctga aataaatgga aatgtaattg attgcagcag
    18961 tttagcttca ggaatatata ttttgcagtt gcatgatatt caaggtcgac ttattggtag
    19021 cagtaagatt gtattgacgg gagtaaatta aacgatggaa ttcgtacatc gtttatcatg
    19081 attttcttta tccaataaaa aaccccggca ttgctgtcgg ggcctccaca tcaaaaaaac
    19141 ataaagcact tgttgttggc ttgttcatgc tttatgtaaa atgtcatcta acctaactgg
    19201 tctgttgtgg ttgtgcagac tatgctaaac tacatcgtaa taaatgccta aggaaaagtg
    19261 gcaacctagt ttaagaaacc ttaaatgtta attaacaaaa aagccccgac attgctgtcg
    19321 aggcttttat gctattttga agcaaatatt atttgcttac agagatttga cggctagtgt
    19381 atttaccatc aacagttgca acttttacga tatagttacc tgcagaaaga gcagttacat
    19441 cgaagttaac agaaccgttg aaagcagttg tattgattac gcgttcgcca agtgcattat
    19501 atacttcagc atataatgtt ccgaagttac cattaacttc tacagttaca ttagtagatg
    19561 caggagttgg gtaaacagaa agagcttgac caagagcata gtcaacaaca ccaacagtga
    19621 tgtcgattac tttacatgca ttgtcgtcat cgtatttgtt catagcagta agacatacgt
    19681 tgaacacacc tgaagttgca taaacgtgag cagggttttt agcagttgaa gaaccgccat
    19741 caccgaaatc ccaagaatat gtatcagcat ctacagataa gtttgtgaag ttcacaagac
    19801 ctgtagttcc gtcaggagta taatcgtatt ctgcagttgg tggataaggg ttaaaacgag
    19861 cctcatcgaa gcaacggaat tgcatattag caattgagtt tacatcggca gcaacttgtc
    19921 caacgtcaag agcagttccc ggctcatcat cctgatgcca aatggtatac atacaaccat
    19981 ttaggaaacg atcgaaagcg taagggaaag aaacttcctg attatcatga gcggtataag
    20041 tcatgttaac aggagcgctc caagaaagac ccatatcatc agaataaaca ccgaaaagat
    20101 cgcggaaaga ctgagcttca gctacagttg gatcatcgaa atagtcggtg tattcaattg
    20161 gcatagtgta agcgaggtag atacgacctg catcttcatc gattgcagca gtaggcattg
    20221 aggtgaaagt aacaccatca tacataccaa ggtcagcgcc gataccatca aaagcatcac
    20281 cgctatcatc ccagtcaacc aaaagatcga tccacattgg tgattcagca gccatagttg
    20341 agttccagta ccacaaaccg tagaggcttg ggaagtagct ccaaccttca gtagccggat
    20401 cgtcgtcgag caaccagtag taaccagacc aaacgtgaac aacaccggca tcatcaagaa
    20461 ccatttcatg gaaaccatca gtagtttcaa ctgtatcagc aataccatcg ccggttacat
    20521 ctgaagtttg atcgaggtca ccttgatagt tacagaaacc tgtattgatg atagtagtga
    20581 ttgtccatga accaggatca ccgttagaag ttgaagtcaa caacacgaga tcagtccatg
    20641 aagtaccgta aaggatgtaa acattgctac catcaacagc gatttgataa gcatcagcag
    20701 ataaagcgcc gaagcagtta gtgctatcca gaggaagtac atcattgagt acagtccaag
    20761 tagcaccacc atcatctgat ctagagaagt tgatagttac aacaggagat gcgttagggc
    20821 taacgaggta gatatcgtca gtacctgcag ggcaataagc gcgtggccac aagccatggt
    20881 gcattcctga tgttggtaat tcagtccatg tagtaccgcc gatagaagca ttagcgaaaa
    20941 ggcgctgatt tgttccatcg tgagcaataa ctacttcatg atccattaca gtaagcaact
    21001 cagggaaacc agtgcgtaca gtttcaacac gagctgttgg tgcaggaccc catgaagctc
    21061 catcattgtg attgtagaaa gaaccacgat cagcccaaga gccgtcggca acggtagatc
    21121 ctgtccaaat ggcagagatt gcgccgtcgt cataaacacg tacacgatcg taagaagctg
    21181 agttagacat cagcgggtaa ccggtaatac cgatagattc acctgtacga gcaccgctct
    21241 ttgcatagaa agtgttggtg ttcagaggtg ttccattgag gtcggcatta acctgaggtt
    21301 gatacggaac agctttggca ttcttgaatt tcgccatcat ttcagccttg ttgtgcggct
    21361 tagccgtcac ggcagtctga gcgaaaagcg aaaccgagat aacgcctgct gctacggtta
    21421 gtaataattt tctcatagca ttaaattttc gttagaaaga cattttgtgt aaagatataa
    21481 agttaccgaa aaatcacata aaaaatgcga aatacatgca atttatagga attgacagtt
    21541 atctgacatt gtcagtggag gcccgtgagt aggggggctc ctggtataga gcaaaaaaaa
    21601 gagggctcca aaaggaaccc tccataaaaa aaattaagct gcttgcttag tttgtagcac
    21661 catttgctgg cgctgcagga gcagcaactt tttttgcagc gggacgaccg cgttttccgg
    21721 cttttttctt aggagcggct tttttagcag cttttttagg agcggctttc ttagcagctt
    21781 ttttagcggc ttttttagga gcggcttttt tagcggcttt tttaggagca gcttttttag
    21841 cagctttttt aggagcagct tttttagcag cttttttctt aggagcagct tttttagcag
    21901 cttttttagg agcagctttt ttagcagctt tttttggagc agctttttta gctgctttct
    21961 taccggcagg acgtccacgt ttgccggcgg cttttttagt tgtttttgcc attggaaaaa
    22021 atatttggtt attgcaaaat tatatataaa atcatattat caaaaaaaaa agaaagtttt
    22081 tttgttgatt ttcacatgtt ttgcacatta ttgtatacaa cctttcccaa tgtgatgtca
    22141 tattcgcact tcatggttga taattgctgc tgaatttctt catacagcaa ttcaaaaaat
    22201 catgattgct tagctctgga ataaaaaata tagtcggtat gtggcaaatc actcacgcct
    22261 tgcatacgca aataattgat gatctgtcca cgatgataac tgccatgatt catacaatgt
    22321 agtatgatct ctccattgcg cgtttgatat tcttgcccat tcaacgcctt gtaaaaacac
    22381 atgtcgccga aaaaatcttc aggcctggag gaaacaaaac gagcgaaatc ttcactctgc
    22441 tgaatgaaat ataaatcgta tccggcaaat tcatcgtcat acatcgcact tggccaatcg
    22501 ctgggtgaag atccttgtaa ccgcgacaac catatcacct gcgcatccca aatatgatat
    22561 aaggtttttc tgatggtggg aaaactgcct cccatatgct tgtcgatgat ctcaggagat
    22621 acactgctga gtgtttgtat gatagcggtg ttcgcccata cattgtaatc acagtaacta
    22681 tttaaaagct ctttcatata tgcaggttaa aaccgaaaga tagtaaactg atgtgtcgtg
    22741 ataatgcaca tcctgtgtat tacggtaagt attttgaggt cttgacttct atttaacaga
    22801 atttaccact gtgcaaccta tcaaacgatc ataatccttg tcaaacatat gcaggtaaac
    22861 cagcaccaca tagtcgtttt ccgattcata ggtattgcct tctgtataat caagcgcata
    22921 gtatgatgct ccctgcggaa gtagggcata ctgataatta taatatcctt gtttcaaata
    22981 cgccttcgcc tcatattgtt gccggtcgta attatatgtc ataggtattg cctgcaaagt
    23041 ggtatcgccc tgcatgcctt cgcctattac aaaaacatta cctgacgtaa tcacataggg
    23101 atattggagg gtgaaatgta tccaggcata atcgccctct ttgtttacat catcagataa
    23161 ctcagcttgt atcacatatt gcccgttcgc atccttttca taagtgtatc gctggtatac
    23221 tctgctttca tcaacattca catacacatg atattgtccg tcctgttgct gtattttagt
    23281 aatccggtcg gtttgaaatt tgagtgtttt ggtatcaaag cgacgatatt cctttccacc
    23341 cggaaatacg agatcgtcga tatcatacat caacatatca ttggtaacca tccgtggttt
    23401 caaattatag gaggcattat caaagcgccc attttgtaat accgttactc gaatgtcgtc
    23461 aaaggcatca tgcacatcga tgttgcggac atcaacattg aaattgatct cctgaaactc
    23521 attgcgataa ggaatcagat tcggacgcat cactttggca tcgatagcgg cttcttgttc
    23581 ccacacaaaa aaccgctttg ttaatattgg tgtatcccta tcctcatcct tccatacctg
    23641 cacaatataa ttcccactca acctgaattg cacatcctca ttaggaaacg atacggcata
    23701 atgtgtgtat cgctgcaagg taccaaagct gaattgataa tcgctgatgc gactttcatc
    23761 aaatccatca atgatatcaa atgaggtcag tccactcggt tcccactgct gatcgcaatg
    23821 tgtgaaagta taatagtaat ctttcactcc tccgtgaagg tcatcaaaac tgagtgtcag
    23881 aaattgacca tcagtattca aagggagtat cggatatgca agctcaagag tgctatattg
    23941 caattgcaca gttctgatca tcggatcgga taccatttct accaaccctt ttttaacttc
    24001 aggtatcatc gtgctgtcag actgtgccga tacacctata tatataggtg tcgacaacag
    24061 aatgcaaaca accttcattc ggcaggagtt aaaaacagat ggcatgctat gctattatta
    24121 caattaggat gccaagataa ttagcgacct ttgtattttc taaaatcggc atatgcaaac
    24181 ctcaaaagaa gccaaactgg aggcattctc acgtttactg gatattatgg atgacttgcg
    24241 cagtcagtgt ccatgggata aaaagcaaac cattcattct ttgcgcaacc ttaccataga
    24301 ggaaacctat gagttggcag atgcaatcac cggaaatgat tggaagggaa ttaaggagga
    24361 actgggagat attcttttgc atatgatctt ctacagcaag ataggtgaag agcaggaaca
    24421 attttcgatt gctgatgtat tacatgctca atgtgaaaaa ctcatacacc gacaccctca
    24481 tatttatggc gatgtaaagg tgcaggatga agatgaagtg aagcgcaact gggagcaatt
    24541 gaaattaaag gagggtaaac gttctgtttt gcagggagtg ccaaacggac ttccggccct
    24601 tatcaaagca tatcggattc aggataaggc ggggcaagtt aattttgaat gggaccatat
    24661 cgatcaggta tgggtaaagg tgcgggagga gatgcaggaa ctacaggaag ccaaagatcc
    24721 ggatcatgtt gaacaggaat ttggcgattt gatgtttgcg ctgataaact tatcgcgtta
    24781 cctgaagata gatccggaga atgcacttga acgcacaaat aaaaagttca tcagcaggtt
    24841 tagttatata gaagagcagg ctgccggcat gcaaaagaat ttgcgtgata tgtcgttggc
    24901 ggaaatggat gcactttggg ataaggctaa agcaaaagag cgcgaagcat aattgccttt
    24961 caattcaatt aaccattagc tttttgtatc ccatgaattc catcaaattt tcaggcagca
    25021 tttcattgat ttgccggagt tcttgaggat ttatcttttt gatttggtca ccactgagat
    25081 gtgcaatttt tttctcgatg gtaaatcttc tgctggcata aactttattg gtactttgca
    25141 ctttttccaa ctccggccat tccactactt gagcaatctg tttcatcacc attgcaggat
    25201 cggttagcag atcatcatat cgcacaaata agatattctt tgcttcacgg gaatagttca
    25261 tccacatctt atagaataat acatactcca taatataatg atgctgatga gccggccagt
    25321 tgtttttgat actccattta ttataacttc ccaaccaact atagggatct tttgacacta
    25381 ttatatatag gtcaggtgcc tgaaacggta atgcagcttc gaagtctttc aatgttggaa
    25441 ccaacaaagt atttgcgaac tgtggctcag gaatgatctc tttttcctta taaagtctga
    25501 agtgtttatg cgtaatctcg ttgcgttggg ttccgttaag aaggaggcca tcgggataat
    25561 ttaattgcaa cagtgtctcc atatagttgg tgccgcttcg ctgtaagcca tataacagta
    25621 tgccttttgc cggaagcttt ttcctgaaga tattgcgcag ccaactcatg aagcacgaag
    25681 atacaaaaca aaaaaagcac ccctgaggat gctttcttgt attgagggtg attggatatt
    25741 gcttaaaaag tttttgtcac tttcatcata taagtactac cgatctttgg agcaccaaca
    25801 aaatattgct gctcatgatt atataagttg gtgatgagca ttgttatctg agtgtctttt
    25861 ataaaatccg gtgtatagtt caacgttagg tccatatcat gtatctcttc aacagtgcca
    25921 acatatgctc cggagtttgc aggataactg ctttgccagc gccaggtgag tccaacattg
    25981 aggtcggcct tcttgatatc ataacttccg cgaacaccaa ctttattttt cggagcattg
    26041 agcgctacat acccgagctg agcgccttca agttcaacag aatctttatc aacccagcta
    26101 taggagaatt gcatggtaag gtcgtcattc acataccagc tcacaccgat atcggtacct
    26161 aaaactgcaa tatcaccaag gttaacataa gtaacatatt tggcaccatc aggatattcc
    26221 attggcgaaa tagttcccac aggtaatccg gcacctgccg caagaacgag gttgatgaat
    26281 tcatctacac cggtcccgtt attatttcca ccgtatgcag gatcatcatc gagtgtagcg
    26341 gtaacaagac ttgcaagaaa ctcgtttgat ccggttgtgt cgtaataatt ttctgtaatg
    26401 ataggtgcta cataagctgc cagttctgca ccatcaataa atacgttcgg cgtgatatcg
    26461 gtaagcggac tcacataatc ctgcatcata ttataatata catcaacggt agcaaatact
    26521 ttatcattaa acatactgcc tttccagcct acttcgatag tattagttgc agaattgagc
    26581 atactgctgt agtcagtgat attggttgga tctattgttt caaattcttc agttgtcagg
    26641 ttcaactgcg caatattgtt ggaagtaact ggaatttcgg gaggaagcca agctacgagt
    26701 gcagcaccta aacccggaat ggtagcatca aatgcatctt ctaataatcc taaagtcaaa
    26761 tcccatataa cattattaag ttcgtaactg ttatcaaggc tgaaataatg atcagatgca
    26821 taacctaaat aagcagagta gggtgaagta tattgtggta aaccaaaatc atcataagaa
    26881 taatggaatc cgtcacgatt gcctattgca tacaatggca tattaggcac gtatgatgaa
    26941 gcaagatatc cttgaaacaa acctgcataa cctgtcaaat cgcctaattg caatatatca
    27001 aggttgaggt tgttgctgct tggagcactg aaggaacggt tgtaagtaag acggatggtg
    27061 tttttattgt ttgccttata cactaatgct gcgcgaggag aaaggaaagg attgtttacg
    27121 aaattatgat aatcatatct caatgcacct actaaactcc atttaggatt taccatgtaa
    27181 tcaccctgtg cataggcgcc gatttcaatg atattatcat catcttcatt ttcgccattg
    27241 atggtatttt cagtatcagg attggtcata atggcgtcta aaccatatac cagatttaat
    27301 tttttatcca tccagtcatt ggcatgttga atctgaccaa catattgctt cgaatggtct
    27361 atgatgaggt cgccggttct aaaaagatag gtgtctccgg catcgctgcc attaacaaat
    27421 ccttgaacaa accaatcgtt atggatgtat cgcaattgcg cataataata tttccatccc
    27481 accgcttgtc cggcaccgat acctgttaat tcaataccat cattttgtgt cattcctccc
    27541 gaaagaacaa attctgcttt gtttttcagg cgatagtcga agcgcagatc aacgccaatt
    27601 ttttcaatgt gactgtcgcg tgtattatca atagtatcgc caacgttttg acgaccttcc
    27661 gaagtttgat agcctaattg tacctgcgca ggttcaaatg gatcatcgta aagccagtcc
    27721 attccgttga ggtaggtgaa aaggatacga tatccgaatt ggtcattcac ttttcctgag
    27781 tgacggaagc cggcagtata aaatgttctt tcgccgatat tgtcgtaatc gaacaatggc
    27841 gtataatcac catatacaag tcctgtagcc ggatcaatag ggttggcgat catgatggta
    27901 tcgcttatca tgcttcgaaa tcctaaaccc agagaggcag aggtcatgta tttcttttcc
    27961 tgacttagag gcgattttgt gatcatatga aatacgccat tcgaacagtt cggaccatat
    28021 aaagcagatg caggtccgcg gagcacttcg atacgttcga ggtcaaggtt gtcggtaggt
    28081 ataaatgttg caacattggc tctaagagaa ggcacggcag catatctatt atctaccatt
    28141 gtcaataaag caccactgaa aatattattg aatccgcgaa caaccacatt ggtttgcact
    28201 aaaccggtat tgatgatatc tactccggga acacccttga tattttgaat tggcgtgatg
    28261 gcaaccgtat ttttaatttg ctcaggactg ataagcgtga tggaggcagg tgcatccagt
    28321 atcttttcct gtcgacgtga ggcgctgaca acaatggcat cgagattata atcttcacta
    28381 gtcagcatta cctcatattt tgctgatgtg gtaagtgcaa cttcaatttc tgcatagcct
    28441 acagatctga ccataagggc ttgtgtatta gcaggaacct caagtttaaa ttcaccatta
    28501 atatcggttg ctgttcccaa agacctgtct tctttggtca ttacactggc gcccggaatt
    28561 gtttcgcctg ttgcagcatc cttcacaacg cccgttactg ttcgattttg tccaaaagcc
    28621 tgagcgccga gcagcataat aaacataatt aagagggtac aaacgtttct catgcaaagt
    28681 ggttttaaag gtaatgtggg tttggttgct aactttacgc ctgtaaatgg cctcaaagtg
    28741 cacaaaaaca ggctattgcg ttatcaaaat tgggttaaaa gttggagaaa gctataatat
    28801 tacctgcaaa taacaaaatt cgaggctgtt gaaaatcagg cgttttcata ttatcctgcc
    28861 aatagttttc gctttatgcg cgataacagc ttatgttatt gccggcagag ctgaacgcag
    28921 tcaaagcctt gaggctacag ccaaacatat tgagcacgat tatcaaaccc tggaacgaga
    28981 gatcattgct gttatttctg atgaaatgat catcagtaac cttataacag atagttacac
    29041 ctttgaagag tttagtcgcc tcgctgaacg tccgtatgga attgttgttt atcagaatga
    29101 cagtgctctc atttggacaa acaatttcat cactcccata aaggcacagg tcccctttaa
    29161 tgatgcgcca ttattgatct ccgaatcgaa tggcgagtat attatatttc aacatgattt
    29221 ccccggcgat gtacatgtga ttggatttct gccattacaa tttgaatatg gcattcggaa
    29281 taagtatctc gaccatgtaa aatctccggg catgcatatt ccggattttg ttagaattaa
    29341 tccgcgtgcc gaaggtgcat ccactcctgt tatgtcgaat agcgggaatg ccgtgtttta
    29401 tgtcagcaat gatggcttgg aaactgctgc aactgtatat ccgcaaagta tgtttatcat
    29461 gctgatctta tcggctttgt tctggttgat gtgcatgtat caacttgcaa gatggatagc
    29521 attcaaattc ggacgattac ccgcagttgg ggcattgttg attttgctgt cgctgagtta
    29581 tatactttta cgcgacctct tttatgataa tgtactgagc gccttaccgc ttttcgaccc
    29641 tcaattttat ggctcaccgg gtattgcagg atctaccggt gacttgctga taaaaactgt
    29701 ctggctattt tgtttcgcac tctttttctc tcgatttttc ttcattaaac aactgcgctc
    29761 tgtttggctg aaggcaacac tcgctatcat tgtggtgttg tgtacattgg tgatgggaag
    29821 cattttcagg agtttaattt cagactcttc tatttctttc gatatcagca atttcttttc
    29881 gctcaactac ttgagcttta tcggattcct ttgtatttct gtgcttttac tcagctactt
    29941 cttaattatt attagatgta tagaactagt aatacgaagt atgcatcgac cactggaatt
    30001 tactgtgtgg gttgcagtga ttagtatact cgctttatta ggtgaatatt ttatcggtca
    30061 cgccctgcaa actgacatgg tctgcatgat caccgggttt gtgttgttaa gagcagtgtt
    30121 gatgaccaaa aaaatcaata ccaggtcgct ggcaggaatg atgatttggc tggtattttt
    30181 ctcagccttc ggagcttata tttttcacac cggtattact aaaaaagaag aggagacaaa
    30241 gcgactattt gcactgaaaa aatctataga aaaagacccg atcacagagt atttatttgc
    30301 aggagcagaa acagaaatca gacatcagtt ggaaatgcag gaaactgccg tagagcaggg
    30361 tagtcgcttg cgtcccggtg taattggtca ggttaggcaa aatctgcagt cgaactattt
    30421 taaaaaatat gacacagata ttttcctgtt tgaaaatgat tctttactca taaccaccga
    30481 tcagcaaaat ccggcaagcc gaatagggtt catcgatgcc attgaaaata atggtgaatt
    30541 tactgctgcc gagaatttgt atttcataaa tgactataca ggaaactatt attatctggc
    30601 ggaattgact acacctattg attccaataa atcgctcaca acatatctta gaatggtgcc
    30661 gaggaaattc agtggcgcca gtatttatcc tgaattgttg atagatgatg atctacgtac
    30721 tgcagaaagc ttcatagatt ttgattatgc tatatacaac gaccactcac ttactgaacg
    30781 cgaaggaaat tattcctatc cattgatgga tatattttac atcaataaag atgaagagtc
    30841 ggccgatatc atggaaaatg actacgacca ctacatctac aaagtgaacg acaataaaaa
    30901 agtgattgtt tcaacacccg ctatcagttg gtttcagcct gtgtctttat tcagttattt
    30961 gtttttcttc tttctggtgt ttttagttat tgtgcttgtt ctcgattggg gcgcgggatt
    31021 attacaagga aaaattagac taaaggagtg gatagatact accttacaaa ataagataca
    31081 atcagcagtg cttagcctta tcattttcgc atttattatg atgggtgtgg caacagtatt
    31141 ttatatcagc aatcaatata atgattcgca taagcagaga ctgcttgaga agatagaagg
    31201 tgtgcaaacg aatattgact ttcttcttgg tgaaaataag cgcaaaactg caggtgcacc
    31261 agtgaatacc caactgaaga aaataggcaa cagggttaca gaattatccg agatacatgc
    31321 catggatatc aatatctaca agccgagtgg cgctttgctg gcatcgtctc aacccgatat
    31381 tttcgacaaa ggattggtgt cgcgaaacat gaatccattg gcttttttta aaatgagttg
    31441 cgataaggaa acatggtata ttcaaacgga acgcatcggc gacctgtcat ttttatctgc
    31501 ctatgtgccg gtattggata gtgaaggaga aaccatgttc tttctcaacc tgccttattt
    31561 cgccactgaa cagaatttga aagcagagat atcctcattc atggttacct tggtaaacgt
    31621 ttatgtgttg ttgcttatcg ttgcaggtat tatagctttt tttgtgagca gaagtattac
    31681 caagccgctt gcagcaattg ccggaaaatt ccgcgagata aaacttggta aatcgaatga
    31741 gcctattttt tggacgcatg atgatgagat tggaatatta gtttctgaat ataataaaat
    31801 gttaaaggag ctggataatt cggctcaatt actcgcgcgg tcagaacgtg aaagtgcttg
    31861 gcgcgacatg gcaaaacagg tggcacatga aattaagaat ccgcttacgc ccatgcgttt
    31921 gagtatccaa catcttcaac gcgccattca aactaatgca ccaaatgtgc aagagcttac
    31981 gcgaaaggtt acggttacta ttttggagca aatagataac cttacacata tcgcatctga
    32041 gttttccaac ttcgctgtca tgccgaaacc tgttaatgag cagatatgtt tgaacgatgt
    32101 tttagatagt gtaaccgcat tatttaatga aacagaaaat gccgccatta cactggaaat
    32161 gccggatgag cgaattatga ttttcgccga caagaatcag gttatccgtg tatttaataa
    32221 tgtaattaaa aatgccatcc aggcaatacc cgatgaccgc gaaggcatta tagccgtgag
    32281 cctggaaagc aatgaacata gggctttggt taaagtgaag gacaatggcc gcggaatcgg
    32341 cgatgaggaa aaagataaag tgtttgtccc taattttaca accaaaagtt caggcatggg
    32401 aattgggctt gccatgacga aaaatattgt tgaaggtgcc ggcggttata tatggtttga
    32461 gtctaccgtt tctgttggca cgacatttta tattgaattt ccgttaatga ccgtgtgatg
    32521 atagcaagag ataaatcaac agccttattt gaagaagcaa aaacattatt tcccggcggc
    32581 gtaagttctc cggtaagagc atttcgcagc gttgaggggg cacctctttt tatgcaaagg
    32641 ggcgaaggag cccttgtatg ggatgaagat ggtaatcaat tcattgatta ttgttgcagt
    32701 tggggtccgc ttattttagg acatgccgat aaacgtgtta cacaagcagt tcaggaagcg
    32761 cttgtaaagg gtacttcctt tggcacgccc acgcgctatg aaaatatcat ggggcagctc
    32821 attatcaata atcataggtt tatagaaaaa ctgcgatttg tttcttcagg tactgaagct
    32881 gtgatgagtg ccattcgcct cgcacgcggc tataccggta ggaataaaat catcaaattc
    32941 gaaggctgct atcatgggca tagtgatgct ttattagtta aagcagggtc cggactcgtg
    33001 acactcgggg aaacaagttc aaaaggagta cctgcagctt ttgcaaatga aactattgtt
    33061 gtggaattgg atgatgaagc agcgatttca tcggcgattg aacaatatcc aaatgatata
    33121 gcatgtatta ttatagaacc tgttccggca aataatggat tgttgttgca acgcaaagaa
    33181 tatttgcagt tcctaagaaa tatatgtgat aaacacaata ttattttatt gtttgatgaa
    33241 gtgatcagtg gattccgact tggttttgaa ggtgcctcgg gcttatacaa catacaaccc
    33301 gaccttatca cgttcggtaa aattattggt ggcggattgc ctgtaggtgc ctacggcggg
    33361 aaaaaagaaa ttatgggaat ggtatcgcct gatggacctg tttatcaggc cggaacctta
    33421 agtggtaatc cattgtctat ttcagcgggg atagcgcaat tgcaagcatg tttgacatcc
    33481 ggattttacg aggaattaca tcgcaaatgc aggatcattg ccgataaagt aaatagattt
    33541 acagccgagg cgaaattgcc ctttaatata cttacaaccg gttctatttt ctggttgaat
    33601 tttacggaag attgcgtaat acgtaaagca tctgatatta aaagcacaat gcctgcatta
    33661 tttaaaccat ttcatcgtta tttgctggag catggtatct atatcggtcc atcgggttac
    33721 gaggtttgtt ttgtttctgc tgcacatacc gacgagcagc tagaacaaac ggcagatatc
    33781 attatgcagg caatcacaca tacattccaa aaataacaat gcccggacaa cgccgatcga
    33841 gatactggtt atttttccat attgtattta tatacattac ggtatctttt ttgtggtggt
    33901 cgtacctgtt gcatcgcaat aatctggata atttcaatga tgtagtcgcg cgtgagaaat
    33961 tcgtatttac ccaagagagg ggtccattcc tgaaatttga ggatacggca aattataagc
    34021 gcatttatga tgattttaaa cgcaccgaat taatgttata tggcgaagga ttggtgtttt
    34081 tatttctcat aggtctcggt ttctggcgca ttcgcaaaac attcagagaa gagattgcca
    34141 tgacgcgcca acaaaacaac tttttgctgt cgattacgca tgaattgaaa tcaccgctgg
    34201 catctttgaa actgagtatg caaacggtgc aaaaacgtca actcgatgcc gaacatatca
    34261 gacaccttag cgggatggct ttggatgaca ttgcgcgact tgaagcattg gttgaaaata
    34321 ttctactcgc ttccaaaata gaaagtgcaa attttcaact taatagggaa ttgcttgatg
    34381 tatcttcatt ggtaacaaat atcctcgctc atattgcaga aaaacataag tcgcaacgtc
    34441 agttcacatc agatatagaa aaagacgcat attgttatgg cgaccgactt tcattttctt
    34501 cggttgtata taatcttatc gaaaatgcca ttaattacag tggtgccgat gggcgtattc
    34561 acgtgcaatt aaccggcgat gaagagcata tttatttgca gatatccgat aatggtattg
    34621 gtgttcccga tgctgaaaaa acaaaaatat ttaacagatt ttaccgcata ggagctgagg
    34681 aaactcgtac caccaaaggt acaggccttg gattatttat tgtaaaacat gtagttgaac
    34741 ttcatggcgg taagattgag gtcactgata atgatccaaa aggcaccaat tttaaggtga
    34801 caattcctgt cggcgcttaa aaggacaata agatgacaat tgtgttaaaa ccctacattt
    34861 acatctgcgt ataattccat caaaccctac ctactatgtt gaaccaccgt attttggttg
    34921 ttgaggatga agatcacatc cgcgaaaacc ttaaactaaa cctggaactt gaaggctatg
    34981 aagtagtaac cgctgcagat ggcgctaaag ctgtaaaaac ctttcgtgaa caacgtttcg
    35041 accttgttat tttggatgtc atgttgccgg agatggatgg gtttgatgtt tgtgagcaga
    35101 taaggcttga agatcgcgaa acacctattt tgttcctgac agccaaagac acatcgcttg
    35161 ataagatcaa aggcctgaaa accggaggcg atgattatat gaccaagcct ttcaaccttg
    35221 aggagctgtt actccgcatc attaatttgt tgaaacgcag cagcaaggcg cctgccgagg
    35281 ataaattcaa tacctataat ttcggtggca atagtgtgaa tttcgttacc tatgaagcaa
    35341 atggtattaa tggcaatgtg aaactcacca ataaggaatt gaaactcctg aaactactca
    35401 tcgacaggaa aaatgaagtc gtatcaagaa cccttattct ccaaacagtt tggggttatg
    35461 atgtttatcc gtcgacccgt accatcgaca acttcatatt agcttttcgt aaatatttcg
    35521 aatccgaccc taaaaatcct gaatacttcc atgccatcag aggggtagga tataagttca
    35581 ccgataaagt ataatcagta gttttcctgc tgataccggc acaaactaag gttatctgag
    35641 gttctgatgc aggctagtcg actctttgtg tgtataaatt tggtattttt acctagtaat
    35701 ccggtacaca cacatgaaaa gactcctctt tttggtagtg ctatgttttt gcatttctac
    35761 tactgcaatt ttcggtcaac tggtcatcaa cgaaggatca aatcgcaatg gtgcggtttt
    35821 aatcgatgaa gatggcgatt atgaagactg gatcgaaata tacaacaatg gtcttgagac
    35881 agatctttat ggctatggta tctctgatga tgccacattg ccattaaaat ggacattgcc
    35941 caatcaaaca atttctgcta acggattcgc tttattattt gcatcaggaa aagatcgcaa
    36001 gccattactc acctataacc attgggaaca acccataagt gatactgcta cttggaaata
    36061 tattattcct agtgccgcaa caccctcaac atggatacaa cctgcgttcg acgatgctgc
    36121 ttggttgtcg ggaaacgcct ctgtaggata tggcgatgga gatgatgcaa ctgtggtgcc
    36181 tgacctcaca cgaagtgttt atttgcgata tcattttaat gttgcggatg taacattaat
    36241 tgaagaaggg ttattgagca tggatttcga cgatgggttt attgcttatc tcaatggaca
    36301 tgtaattgcc atgtacggtt ttgctgctgc ttctcccgca tataatgcat tcagcgacag
    36361 cgatcatgaa gctgccatgt atggaggtgg agtgcctgaa cattttaaca tcagtgcaga
    36421 ttctattaat gcatggttga tgacaggtgg caatgtcctt gcagttgaag tgcataatgt
    36481 atctgcaggt tcaagcgatt taactgcacg tccgttttta agtcttgctg ttaccgtacc
    36541 tgatattatt tggacagatg ttttaccgga atggtttcca ataggtggcg gaacttcatt
    36601 tttgcataca aattttacga tcgccccatc cggtgaaatg atttatttga gtgatcctgc
    36661 cggattaatt atagatagct tatttgttgg tgttgccgac gcggatcagt caaacggcag
    36721 aattactgat ggagcagcgg atatgcaaat tttcactaca ccaactccaa atgcatcaaa
    36781 tggtggaacg tcataccccg gttatacttt aggcgatgtg acatttactt tagatgcagg
    36841 attttattcc ggatcacaaa caataggcat tacacatcct gccggaacca ttgccagata
    36901 tacattgaat ggcgaaatac caacttccac aagcccgatt tatctggtgc caatcaccat
    36961 tgatacaaat actgttttta gagtaagatt atttgatctg ctaggtgaat tgctgcccgg
    37021 tagaacattc accaatacct attttattga tgagaatatt accactgccg taatttctat
    37081 ttctaccaat gaagataatt tgtacggtgc aaatggcata tttgataatt ggtggacaga
    37141 ttggaaaaaa ctgtgctacg tagaatattt tgataccctg ggaattaatc aatttgaaca
    37201 acaatgtgga tttaaggtag atggcggagc aggtggcagc cgtagtcttg cacaaaaaag
    37261 tatgcgcata gaacctgata atcctgcctt tggcgatggg actttaaatt acccgctcat
    37321 acctcgcaga tgggataccg atgaatatga aacattttat ttgcgcaacg gcagtaatat
    37381 gagcaatgta ttgccatata aagatgcctt tatggtaaga acaaccgaag gtacttacaa
    37441 tgagcacatg gcatatactc cggtagtagt atttatcaat ggcgcttatt ggggtgtata
    37501 tgaattgcgg aacaaattgg atgaaggcca ttttgatcat gtaaaaggta ttgaaaaaga
    37561 cagcctcgac ctgatgacac tcagttattg gtatgggttg gtactgcgca cgcttagcgg
    37621 atcggataca gatttcattg aaatgcgcaa ctacctcggc acttatccga cacccgccga
    37681 tgatgatttt tatgatatcg ccgattctat tctcgacctc aacaatttca cggattatat
    37741 catatgccag acatggtttg caaactacga ctggccatat aataatatca aagcctggcg
    37801 cgacagggga ggagataaca aatggcgcta cgcaattatt gatgtggaat tgggacttgg
    37861 aatcggtgga tggtctgatg cgaatgccaa tttaattccg ggattatttt atacccaaca
    37921 atatatcgag ccgctggcaa ccttacttca gaatccgaaa taccgtgagt attttataaa
    37981 tagatatgcc gatctgatga atagcaccta tctcacagat cgtacattaa gtatggaaga
    38041 ttctatttat aacgaaatat atccggaatt accgcgacag cttgaaagat ggggctacgg
    38101 cgcagtggaa gatcaactcg ctactttcga agattatcgc aatgccttgc gcaacgattt
    38161 tagtatcaga acgaataaag tgcgcctcca tattaaagat ggatttgcac ttgaaaataa
    38221 agtcgatgtc accttaaatg caactcctgc aggatgcgga catattcaga tcagcacctt
    38281 ggatatttac gattttcctt ggacgggtgt ttattttaat ggcaatgaag ttcaggtaac
    38341 tgccgtgccc cttcccggtt gcacatttga tcattgggaa gcaaatccat ttattaccga
    38401 catttataat cctactttta aagtgaatat cgatgcctat acaacgacat tcactgccgt
    38461 ttttacaggc acgccatatg aacagaaaat cgctgtcagc gaattaaatt atcaccccga
    38521 atcatcatta gatgacggca attggataga aattgtaaac gccggtgctg atcctgtttc
    38581 ccttgatggt tggtatattc gtgatagcga accctatcat cgttatgata ttcctgcagg
    38641 cattgaatta aatagcggcg atagatgggt gtttgctgat gacactgcga aatttcattt
    38701 attacatccc gatgtaagta atgtatccgg gccattaggt tttggcatag gcgattatgg
    38761 tgaagatatg ttgctcataa atgcacgcaa cgaaattgtt gagcatatgc attacgatga
    38821 tgctgctccc tggccgcttg gtgccgatgg tcatggcaga acactcgaac gaaatgatat
    38881 gtatgccgat gtgaacgatc cactaaattg gttcgacggc tgtatcggcg gttctcccgg
    38941 cttagcttat tctccatgtg aagaccctat cgttttcagc gaaataannn nnnnnnnnnn
    39001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39061 nnnnnnnnnn nnnnnnnnnn nnnnnnntct cccggcttag cttattctcc atgtgaagac
    39121 cctatcgttt tcagcgaaat aaattataat agcaatgctg cgttagatgc cggcgattgg
    39181 attgaattgc ataatgtcac cgatgaacca ttgcatatcg gcgaatggat atttatggat
    39241 gactccatcg gcaccgaaca tcaatttatg ataccttccg gaacaacgct tgaaccacat
    39301 gcacaccttg tacttgctca ggatcttgca ttattcaatt cagcacatcc cggagttacg
    39361 aatgtgatcg gtggatttcc tttcactttc agcggtaacg gcgaatggct aagattatat
    39421 gatcaatctg aggttttgag ctttgccgtt gattatcagg atatcactcc atggccaact
    39481 gtagctgatg gcgcaggata tacactcgaa ttggtcgatt cacttggtgt aatgaatgat
    39541 ggcaataact ggacagccta ttgtttggga ggatcgccgg ggacatttgt aatagaacca
    39601 tgccctgaaa ctattgccat aaaccaaatt agtgatatac aatcgcaaat ttcggtgtac
    39661 cctaatccgg taaatcaata tgcacttaca gcaatttcta tagaatatcc tgttaatgca
    39721 tcaataaggt tgtttgatgc acaaggaggg cttcgtggca ctctgtttga cggtatcttg
    39781 caggcaggta gttttgcaat ttctttagat atgtcggctt acccggcggg tatgtatttc
    39841 ttgcagctgc aatcagataa taaacaatca actattgcag tgatcaagca ataatcattc
    39901 ttgtaataga acctctttca atttttcgtg tttatccatt tacctttgca atggcaatat
    39961 gatgaaaaag aaaggcattc tgttagtaaa tcttggcacc cctgattctc cggcaacagc
    40021 agatgtgcgc agctacttga agcaattttt gcttgatccg cgagtaatcg atattcccgc
    40081 cattccacga aatttattgg tgcgtggtat cattgcccct tttcgtgccc ctaaaagtgc
    40141 tgcgctgtat aaaaaaatat ggaccgatga gggttcacca ttattagttt atagcaaaaa
    40201 acttcatcaa aaaattgcaa ctgcactcgg cgataactat tcggtagtac ttgccatgcg
    40261 atataaaaat ccgtcaatag aaagcggatt gcaattgctc gccaaccagg gatgtactca
    40321 tatcactgtg gttcctttat ttccgcaata tgcatctgct acaaatggtt ctgttgttga
    40381 gcatgtaatg gcaattgtca gcaagtggca aagcattcct gatctgcatt ttaccggtcc
    40441 gttttatgat catccaaagt ttatacaagc gattgcgcaa atagcatcaa catatcaatt
    40501 acaggactac gatcattttt tgttttcata tcatggcgtt cctgaaagac aaatacacaa
    40561 ggcggcaaat ggaaatgcgt gtttggcaga aggatgttgt gatgtcatgc ataaagacaa
    40621 tgccttgtgt tatcgggcgc aatgttttca aacaacgaga cttattgcgt cggcactaaa
    40681 tctgcctaca gacaagataa ctacctgctt tcaaagtaga ttaggtaaaa ctccatggat
    40741 acaaccgtac gcttctgaaa caattaccgg tcttgcagca gaaggcaaga agaagttatt
    40801 ggtgttcagt cctgcatttg ttgccgattg cctggaaacg atacatgaaa taggcgtgga
    40861 atacgatacg gaattccgcc atctgggtgg tgaaaaagtg caactaattg aaagcctgaa
    40921 cgacagcgac ctgtttgttg aatgtctggt ggatatggtc aaataatcat cagaacatat
    40981 ctacaccacc atcataattc tcatccattc ctctgttacc acgtcgacgg ctgttatcct
    41041 gctggccgaa tttgtaagtt agcttgagac tcaatatttg cgattgacgt ttgcgtgaat
    41101 attcggaata gaaattcgtt ccctcgctca caccgctcca ttttctggaa ttgaaaatgt
    41161 cactgaaatt cacgttcaca cttaatttat tttggaagaa atcggatttg acacctgcat
    41221 ccataaaata aatcgcgttt gactcgcctt gcgcgaaggt ccatggtgta ttgtaactca
    41281 tggtcaattg cagactggtt tgcttataca ccgcaaacat atgcatgagt cggatattgt
    41341 aagtgaattg gttatttgta agtccggctt ctaaattttc agcgttaaca agcgtttggt
    41401 tgaaattgaa actaccggtc atattccacc acttaaagaa ctcatcacgc gttaccagct
    41461 caaatccgta tttcgattca tcattgtaat tctggaaggt catgtatgat acaccttcgg
    41521 gagttactgt gatatatggt gaaatttcat tcaatgtttt ggtgtaatac aatgatgctg
    41581 taagtgaatg ttttttgaat tccttctcaa aaccaatttc atacgagttt tcatactcag
    41641 gatctaaaaa tggattgccc tggcgaaaac tataaggatc tgaataatcg cgaaatggat
    41701 ttagaaacca ggcattcggt ctgtcaatac gtcgactata acttgccgag aattccatag
    41761 tttcatcaac tgcatagcga acatgcgcac ttggaaataa tccaaaataa tcatgtgtga
    41821 aatcttcatc ggttgtaatc agttgcgaca aggtatatgt ttgttcggca cgcaagccaa
    41881 tttgatatcc gaatttcttt ttgaatgtgc cattccaaat caaataacct gcaataacat
    41941 cctcattgta tatgaattga ttatttaatg aatcgtctgc attccagctt gcaggatctg
    42001 caccaatgct ataaaaagat tcgctgtaga atgtgttatc ttttttctct tgtgtatatt
    42061 ttatacctgt ttcgaactga ttaccattct taaatggtcg cacataatct gattgtgcag
    42121 tgatgtcaat gttctcgtta ggtcggctaa tattttgagg tacgaggcta taccaaaagg
    42181 ccggtgtagc gtcataaaaa tcttcggcat aatatgttga atcatcactg ctaccgctgg
    42241 cataatatgc ttcagctgac catacatgtt taggatcttt gaaggtatgc ctgatggtag
    42301 caccaccatt ccatccaatg ccattattgg ttccggaagt tgtgcgctta ctggtgcttg
    42361 ttaatgtgct gtcaatgttt agaaaggagt aatcaactaa tccggtattc gatcctgcat
    42421 tgccatgcca actgccgctc acgcctagtg ttgtgtgatc tgtgaggtaa taatcgattc
    42481 cggcacgcgc catgtgcata ttggaaatat tattacttga gccatttgaa agaaggtaag
    42541 aaatggtatc ttcctcaaat gtcttccgat aagtatatga ctctccatca cggtcatcgt
    42601 gcgtgaatga ataattggaa taaatattca cttttttatt gcgatagttc aacgatgcac
    42661 ctgcttcata ccgcggtgat gttgatacac ctgcagtaat atttccgttc agcccaattt
    42721 ttttattttt cttcaacaca atattgatga taccggaatt tccttcagca tcatatcgtg
    42781 cagaggggtt ggtgactact tcaatactct ctatggtatt tgccggaatc tgctccagaa
    42841 tggcattcat atttgaacct gctatgccgc ttggtttacc atcaataaaa atgcgtacac
    42901 cttcgctacc cctaagactg atattaccat caatatctat cactacactc ggaatggtct
    42961 gcaatgcatc tgacgctgaa ccattcgaag cgatgatgtc cttttcaata ttatatacct
    43021 tcttatctat gctgccttga aaatatcccc tttcagctgt tacttcaacc acatccagta
    43081 atcccccatc agctgctaaa gttatttttc caagatcagt aaatggattt ccgggtgtga
    43141 tatcgagggg taatatcttc cttttatagc ctacgtaata caattgcaat tcatatttgc
    43201 ccggagtagc tacctgaatt ttgaattttc ctgcttcatc ggataaaact ccggtaatta
    43261 tggttgaatc gccgggatgt tttatagtta tcgtgccgta ctcaagaggt ttggcatttg
    43321 ttgaatccaa taatgttccg ctaatagtaa tcccgggtat ttgtgcttct aattgctgta
    43381 ctgatgtaag catgaggagg aaggcaaata cggcaaataa tcttgtcatc atttcaattg
    43441 caaaagtaag gatttcggcc ttgggaacca tgcgattcgc agtgccataa gccattgtaa
    43501 caaaaattaa cctcaaatgt tccctaaaac aattggtgat aatggtcatt attatcgctt
    43561 cgtatggcta aggtttagta tttttcgggc tgaaactgaa atgcccgtgg aggataagat
    43621 caaacgatta catggtatgc aggctgaagc cctgcttgga ggaggtgccg aacgtattgc
    43681 atctcaacat aaaaaaggaa aactgacagc gcgtgaacgc gttcatctct tgatggatga
    43741 aggcaccttt gaggagatcg ggcaattggt ggtgcaccgc agtaccgact tcggcatgga
    43801 aaaacaacaa ttctatggcg atggtgttgt aaccggctat ggcaaggtaa atggccgctt
    43861 ggtgtatgtt tatgcccagg atttcacggt gtttggaggc agtttgagcg agactcatgc
    43921 cgaaaaaatt tgccggatca tggatcttgc catgcagaac ggcgctcccg ttatcggatt
    43981 gaacgacagt ggcggagcgc gtatccagga aggtgttaac tctcttggtg gctatgccga
    44041 tctcttttac cgcaataccc tcgccagtgg tgtgatccct cagatttctg caatcatggg
    44101 cccctgtgcc ggcggagcag tttattctcc tgccattaca gacttcattt tgatggtgga
    44161 acgcacctcg tatatgtttg taaccggacc taacgtggtg aaaacagtta cgcatgaaga
    44221 agtttccagt gaagatctcg gtggagccgt tacacatggt tcaaaaagtg gtgtatcgca
    44281 tttcaccgct gcaaatggcc tcgattgtat ccagaaaata aaaagactgc tatcctacat
    44341 tcctcagaat tgcgaagagg aatctccggt ttatccatac acaatgggtg atgagttgcg
    44401 tccagcattg gatcgtatta tgcccgaaaa cccaaatcac ccttatgata ttcgcgaggt
    44461 gattactgaa gtggctgatg tcgacagctt tttcgaagtg catgaaacgt atgctgaaaa
    44521 tattgttgtt ggctttgcca gagttgcagg aaagagtatc ggtattgttg ctaatcagcc
    44581 cgcagtatta gccggtgtat tagatatcga agccagtaga aaagcggcgc gttttgtgcg
    44641 tttctgcgat tgttttaata ttccattggt tgtttttgaa gatgttccgg gattcttacc
    44701 tggcaccgat caggaatggc gaggcattat taccaatggt gcgaaattgc tctttgcttt
    44761 cagtgaagca acagtacctc gcattaccgt tattacacgt aaagcatatg gtggcgccta
    44821 tgatgtaatg aacagcaagc atattggtgc cgatatgaat tttgcctggc catcggcaga
    44881 aattgccgtt atgggaccaa aaggtgctgc tgagatcatt ttcaaaaaag aaattgaaga
    44941 tgctgcagat cgcgatgcga aattgcagga gaaaattgat gagtataccg aaacctttgc
    45001 aaatccatat cgcgccgcat tccgcggttt tgtggatgag gtgataaaac cttcggaaac
    45061 acgtatccga attatccgct cattggatat gttgaagaat aaagtggcag tgcttccacg
    45121 aaaaaagcac ggcaatattc cattgtaaat taagtcttta ttattcatgg ctaactctaa
    45181 acgcaaaaca tcactgattg gcgacaagga aatagatttt ctgcgcagct atattaataa
    45241 tccttcacct acaggtcacg aagctgcagg tcaaaaaatc tggcttaatt atattaaacc
    45301 tttcgtcgat gatcatattg tcgataatta cggaagcgtt gcagccatta ttaatccgac
    45361 agctcccttt cgcgtagtaa tcgaagcaca tgctgatgaa atcggatggg ctgttcaata
    45421 tattaccgaa gacggattta tatatgttgg acgtattggt ggaagcgatc atattattgc
    45481 accaagtaaa cgagtaaata tccatactgc ttccggtgtg gtgaaaggtg ttttcggttg
    45541 gcctgctatt catacacgca tgggaaagga agaaacgcct gatatgaaaa atatcttcct
    45601 cgaccttggt tgtacatcga aaaaagatat tgaaaaaatg ggcgtgcgca ttggcgatcc
    45661 tattactttc gatgatgatt tcttcgtgat gaataattat tatgtgggcc gcgcaataga
    45721 taatcgcatc ggcggattta tgatcgctca ggtagcaaga atgataaaag aacagaaggt
    45781 gaaattgcct tttgctttat atattgtaaa tagtgtgcaa gaagaagttg gacttcgcgg
    45841 tgcagaaatg atggcgcaaa caattaagcc acatgtagct atcgttaccg atgtaacgca
    45901 tgatagcaat acaccgatga tctccaagac gcttgaaggt gatattaagg ttggaagagg
    45961 tcctgtactt acaaaaggcc cttctgtcca caacaaactg cttcaattga tgcaggatac
    46021 agcagatgcc aataaaatac cataccaacg cgattttgcc tcaagatcga caggtacgga
    46081 cacagatgca tttgcctatt cgaatggtgg agtacctaca agcttgattg cgctgccatt
    46141 gcggtatatg catactacag ttgaatccgt gcataaggat gatgttgaaa atattatcaa
    46201 actcatgttc tacgtattga aatcacttaa accgaatttc aatttcaaat acctttgaga
    46261 atttacgtaa tcaacagtta ctaacagggg ctttaaggaa tgagcattca gataattgtt
    46321 gcggcttttc tactatttgt atttcttccg tttattgcag cggttattat atttattgta
    46381 ttcgcccgga ggaaaaagcg ggaggatagg gcagctggaa tcttgcctcc aaagaaaaag
    46441 aaaaagcctt ccattttcag aaggctcttt caatattata tcaacagaca agcgtcagtc
    46501 aggaagtaat tctgccagtt ttttctccaa tgctcctcca cgtaagtttt ctgctatcac
    46561 aataccattc tgatctatca gaaacgacat cggaattccg gtcacacgat aatccgatgc
    46621 tgccttattg ttcttctgat ctagcagaca aacatggttt tcccatatca ggccatcttt
    46681 cttgatagca tcaagccatt tatttttatt atcgtcaagt gaaacactga aaacagtgaa
    46741 tccctttgat ttgtacttat tatatgcttt taccacatta gggttttcct gcctgcaagg
    46801 gccacaccag ctagcccaga aatcgagtaa taccactttg cccttcaggt ccgacaattt
    46861 gcgcatgttt ccttttgggt caggttgttc aacgtccata aaaggtttgc ctacaatgct
    46921 attggctaat tcagcttcac gcagtgttat tttctctccc atttccttga gatatacaga
    46981 agatgagtcg gcagagttct tcgctttgtt gtagtatgac ttcacataat cgaagtcgaa
    47041 atcatacata gattctaatg caagcactgc attggaagtt acggtagtag tatcgacata
    47101 attcttgaaa attgcgaagt agttcaattt catatcatca agaatggcgt ctacaataga
    47161 atctttgcca agtgatttta gcgtgcgcaa gctatcattc atcatacccg cttcagtatc
    47221 aaatgagcga agcgcatcca ttaatccgaa taatgaattt gtagcagaac taccttcaac
    47281 cttcggatta cccggatctt tccagctggc atctattttt attttttcat tatgtacaga
    47341 taaaatgtaa tagttcattt gatcgccatc gattgtcatg cggcatatca tgcgctccgg
    47401 caggtagttt tttatttcaa aatttcctat attatctgtc cacgcagtat ctattactac
    47461 aaaatttggc tgaccgtttg caggaaaggt catgctttcc aataatacct ttttattttc
    47521 cgcgcctttg atcacaccgc ttatttcgaa ggcctcagat ttttgtgatg cacaggaatt
    47581 cagtaaacca ccgatcagaa gtggcaataa aatcaaactt aattttttca tgtttatgtt
    47641 aatcattttc agatttgtat taaatcaaca gtattgaata ttaaatagtt caggcattga
    47701 gtttctcctc caacaagcga gttgccagtt tgggatctgc ttttccttta cttaatttca
    47761 ttacctcacc cataaataat cccaaaatgc ctttctttcc actgcgatat tcagcaactt
    47821 tttcagggta ttttgaaata gcttcttcaa ccaatttctc tatctctccg gcatcacttt
    47881 gctgtatcag attcagtttt ccggcaagtg cttctgctgc tatttctgcg ttttcaagca
    47941 ttgcaggaaa caattgctga ctggcaatgg tgaaattcaa ttttccggat tctgtcaatg
    48001 caatgagttc cgctatctgc gcaggtttta ttggaagcgc ttcaatattt tccgcatgct
    48061 cattcaacca actttttacc ggaccctgca tccagtttgc tgccgctttg aaattatttg
    48121 taagtgcaat taattcctca aaatacaatg cgatatcgcg ctctgcagtt agaatttccg
    48181 catcatacac gctcaacttg cactgttcta catatttatg catgaggtcg cgcggcaaag
    48241 cgggcatatt agcttttaca tcggcaatat gtgcatcact tactataaaa ggttgtaaat
    48301 cgggttcagg gaaatagcga taatcatgtg cagcttcttt actacgcata ctgaaggtgc
    48361 ttccttttgc ggcgtcgaaa ctccgggttt cctgcacaat ggtttcaccg gcttcgatag
    48421 catcgatttg tcgctttatt tcataatcaa ttgcacgctt tacgttgcgg atggagttca
    48481 tatttttcac ctctacctta acaccaaatt ctgctgctcc ttttaatcgg acactcacgt
    48541 tggcatcgca acgcaaactt ccttcttcca tattgccatc gcaaatatca aggtagcgca
    48601 ctaacctgcg aatctcagtg agataggcat aggcttcatc accactgcgc aaatcgggct
    48661 cagaaacaat ctcaaccaga ggcgtgcctg cccggttaag gtcaaccagg gtgtaaaacg
    48721 gatccaggtc atggatgctc ttgccggcgt cttcctccat gtgaatacgg gtcaatcgga
    48781 ttatgcgttc ctgtccatag gccccgatgt ctacatgccc accggtgcat attggggtct
    48841 tgtcttgcgt aatctgataa ccttttggaa ggtcgacata aaagtaattt ttacgggcaa
    48901 actcattgta acgggtgatt tggcaatgta aggccaatcc cagtcgcatg gctagattca
    48961 ggctctgagc attgaaaaca ggcagggttc ccgggtgacc aagcgtaacc ggactcacat
    49021 aactgttggg ctctccgcca tatgctgcaa tatcggaact gtatgccttg gtgcgggtga
    49081 gcaattgcgc atgcacctcc agcccaatca cagcctcata cttatcgtac acttccataa
    49141 gggcgcaaag ataagcaata ccgccgcctg aaaagagttt gaggaagttt gacccaaagt
    49201 ttggatttta aaatttttgt tatcttcgac ttggatacag ttggatttct ttcatatgta
    49261 tactaaccgg tgtcggaaga ggagctggca gccggttttt ttatgtacct atatcgccct
    49321 agatcttcca acaatcaata tgcattccgg cagtaaatgt gaattataca ttgggctgac
    49381 aatttagtac caatatcaac aaattcatga cagacagctc gattaatagg ttttaatttg
    49441 ttctcaaatt acacatcatg agaactttct tacctgcagt atttttattt tttacaatca
    49501 ccgtttcggc tcaattgcaa ttgccattgc aagcaaatcg ggttagcgca ctcttttctg
    49561 ctaatggatt tttatttact gatttcagct atatggctca gtatacagta ccgggatata
    49621 gtgcaggtca ggaaaataat gccacaattt ttgccggtgg attttggatg ggcggttatg
    49681 atgatgccga tcaactccat cttgctgcca gcacatatcg tcaaaccggt gatgattatt
    49741 gggcaggccc tatcgcaaca aattatacaa atccggatta tattgacaga tattcaaaag
    49801 tgtggcccgt agatcgagct gaaattgaaa atcatattgc caactgggac gtgccgggat
    49861 atttagttcc accatcaatt gcgtattggc ccggaaacgg gaatgttgct aacggcgaag
    49921 ccgccattct tgcccctttt tatgactata acaacaatct aatctatgac cctgaaaatg
    49981 gagattatcc attggtgcgg ggcgataaag ctatatacat gattttcaat gatgccgccg
    50041 aagaacacgg tgaatcaggt ggagaagcct tgggcgttga attacacgtg atggcatatg
    50101 gatttgatat gccggaagac agtgcattga atcaaaccgt atttgtaaat gtttcggtaa
    50161 tcaatcgttc agttaataat tacaacgaat ttattttcgg aaattttgct gatttcgatc
    50221 tcggtgcata tgacgatgat tatgttggat gtgattcaaa tttgaatgta ttttttgcct
    50281 acaatggcga tccggttgat gggccgacat ttggaaatta tggatcaaat ccacctgctc
    50341 agggtgtcat ttttctcaat cgacccatgc acgctttcaa atattataac aacgatttca
    50401 gtgtaaccgg aaatccgaca acaggagaag atttttataa ttacctcaat ggtaaatgga
    50461 tagacggcac gaatcaaacc gaaggtggaa acggttatgg cggagcggtg ccaatgaatt
    50521 tcatgtatcc cggacatccg atggatcctg atgcgtggac agaaatgcgg gaagaaaatg
    50581 tgccttccga caggcggggg gtggggtcga taagaccggt aacattaaat gccggtgata
    50641 gtatgtgtat cgagatggca tatctttctg cttttgtttt tgataccgtc aatgctgaag
    50701 gaacaactac tgatgtgcat gcattaggtg caacggaaat attactgaga agaactgttg
    50761 atgtaattga ttattacaat gctaattatg aagcatgtca gattgcctat cagtcgaatc
    50821 cgaattattc ttcgatagtt gcgagtgata ttgcaaataa tatatcaatc agtccaaatc
    50881 ccgccaatga tttcatagaa gtgcaattgg gtggagaaaa tgttatcggc accattatgc
    50941 tcatggataa ccaaggacga aggatctatg aaagtacgct ttcagggcag ccatcaataa
    51001 gccttgaaat aactgagtat cctgcaggga tgtataactt gctcattatt cccgaaagtg
    51061 gtgctatttt atcggcatcc tttattaagc attaacaggc ttgctttttt ggtggtttgt
    51121 tgcagtagaa atgcccggta tctcaatgat gtcgggcttt tgcacataat tcacggctat
    51181 tgccatagac gtttggtaag ctatttgtaa atttgtaaaa tgggcaagtt ccgacatctc
    51241 gtatggctga tgctgctcac ctctgcattg catgctcaac ccggcatgct aggaacgcag
    51301 gcggtgaacg gcagttacac ctcgtacagc ctgaacgata tgggaatctt tcgccaaatg
    51361 cgcgtgcagg caacgtcgtc tgccgcctcc ggaacacgta actgggaatt ttatgaagca
    51421 gcagcagatt acgatcctgc atggcgaccg tatttttctg caatgacgct atcgggatac
    51481 aatcagccaa ttgcaccctc gggcgcttcg gcatctgcaa cttataatct cggatttggt
    51541 ggagcaagcg gattactgcc tgccgttaca tccggaaatt attacacttt caacgtaacg
    51601 gaaaaatcta cgcccggaac gcctttagat gaacatatgg gggtgatgga aacatcctat
    51661 aatccggtgg caattacctc tgttacgcaa tcaccgggaa taggcgcagt ttatccggaa
    51721 aattctgttt atgtcactgt tacattggca tcgacattgc atgcagatga atatgtatat
    51781 gtaagatatt caacgacatt taatttcact tcttcctcat tattacctgt taccatatca
    51841 ggcaccacag gaacagttga aattccatgt caggcggcat ctacaactat ttattattat
    51901 gcgtatacct ccaatagaac ttcggcagaa attttggctg atgttgctat tcctgctatt
    51961 ggagaatatg cacatgatat gagcaccctt tgcatcaata ataacggagg tccaaattac
    52021 agttatacgg ttttaccaag tatcggtttc tgcggttctt attatattcc tagcacctgt
    52081 tatccaacca ttaatagctt tgttaccgca ttaaatgcag gaaccgttgg ttgttctgtg
    52141 atatgctatg ttgctgcagg acacacagaa acagcacctg caggtggaat taatcttacc
    52201 cagacaggaa cagcagttaa gaccatcaca ttcataaaaa acggtgcggg cgcgaatccg
    52261 attatttatg caccggtagg tacggtgagt atagttgccg cttccactgt tgcagatggt
    52321 gtgtttagtt tgaatggcag tgattatatc accatagatg gcatagatat aattgataat
    52381 aatactacag gtgctaccat gatggaatat ggatatgcat tattcaaagc atcaaatact
    52441 gatggttgtc aaaataatac gattaaaaat tgtgctatca ctttaaacaa taacaattca
    52501 tggacaggcg ctccgctgaa ttttgaattt ggtgctacgg gtattatgat gcgtaatact
    52561 gcgcgaacag cattaacgac attatttacc attacaggtg tgtccggaag aagtgataat
    52621 aaccagtttt atggaaatac aataacaaat gttataaacg gatttgtatt atcaggatac
    52681 aatgatgtta cgtcaccata tacctattat gatcaaaata actatgttgg agtagggggg
    52741 ctgggtaata ctatcgaaaa gtatggtaat acaacggcta cagtgaggtc atcaggtgta
    52801 tatgcaattt acaacaatgc gctggttgta tccaacaata caattggtaa ttacttatca
    52861 ggtggaactg cacataataa cactttatat ggtgtgtttg tttcaagccc aataagtgga
    52921 aataaccttc aaacattaaa tattcaaaac aacactattt cactgaaagg acataccggt
    52981 tcaagcagag ttacggggat aaaggttggt aatgcatcct gtggggcaac atctataaca
    53041 attgatggaa atactatcga agattgcaca ttcactgccg gatcaacagg tgagttttat
    53101 ggaattgata tcggattcaa tgcaactgct gtagctatta caaacaatat cattcaaaac
    53161 aatgtaatca acactactac cacaagcgtt caatatttga tttataataa tactaccggt
    53221 tcggcaatag atgtttcata taatcaaatg ctgaataatt ataaaacggt atccagcacg
    53281 gccggtattc tttatggcta ttattcagct tccggatctg ccaccggtac tcaggttttt
    53341 catgataata tcatagatgg tttaggcatc ccttctgcac aaaatggaag tgtatgtgcg
    53401 gtgcggattt cctcatccat aaatcaaagc aaggaaattt ataataacgc cgttagcaat
    53461 ataacctccg gaacaagtat tgcaactatg tatagttgtg gctattacat agatggcatg
    53521 aatgtaggcg ccacggtgca tgataatagc ttgtcggatg taacctgtgg caattatttc
    53581 gccgggttta atttttgcag tgcggcatcc accgcatcta ccgctaatgt ttcgtattct
    53641 tgttataata ataccatatc gaatgttaca acaacaagca caaataccgg gccgcatggt
    53701 attgctgctt atatgcccac aactggttcg ccggtgataa atatttacaa caataatatt
    53761 accggcttaa attctacggc ctccttttcg ggatatattt atggaatatt tatgggagga
    53821 ggttctgcaa atgctacctt gaatttttat gagaatacgc tccatgattt tgtgcatacc
    53881 ggtgctgcta ccaatacgat agtggcaggc gggctaattg cattgggtgg ttctacggtg
    53941 aatttctatc gcaacagcat aaatcatatt acaaatacag gtagcaatgg catctcttat
    54001 ggtttatata ttacaagtac gggtgtagta aatatttata ataattttat tcagcgactg
    54061 tcatccactg caactactaa tgtgataggg gtgtatggca tttataccgg ttccagcggt
    54121 gggttttggc aatttgtgaa taataccata gcgctgggtt atgacacacc attaacatca
    54181 accggcgcca atttcggatt taccggaatc tattttgccc ctaccggcgt tactgccact
    54241 gttttgaata atattgttta tgtacgtgga acagctaatg gcaccgccct gggatcttgt
    54301 attcgcggaa gcattggcac cgcttataca ccgccggctt cattttcatt aagtaataat
    54361 aattattatt tcattaataa tgcaacctat aattacatat atacagaagg cggattcaac
    54421 acatctaatg taaatggata tgcatatggc ggggctttta ctgatgtaac caggaatttg
    54481 aataacgacg attgttttaa cgatccggcc accgggcttt ataaagcatt tatgtcgccg
    54541 gcagaaagca atagttatta tgatgtgcca ccattcgcag gagggggcag ttatccttat
    54601 aatttaaagc ttaccaccgg tgcaactact tatgccgaat cgaatgcatc tgcaatcccg
    54661 ggaataacca ttgattacga gggcgatgca cgttcaggtt ctacaccgga tatcggtgcc
    54721 gatgaaggag attttgttcc tcaggctgtt gattgttttc tattgcctat tgagttaatg
    54781 tcatttagcg gcgaaatttc aggcgatgat aacctgctta tctggactac agctactgag
    54841 cataatacta gttaccatat cgttgagaga tcagttacag gttttgattt cacggcaatc
    54901 ggctctgttc ctgcggcagg tgaatcggta tctgaaatca cctacaattt tgttgatcag
    54961 catcccggca gcggaatcag atactatcgc atcagaaccg ttgaccttga cgagagtaat
    55021 tcgttgagta atgtgattgc gctggatcgg gagcttgcta atagcactca gac
//
LOCUS       DAOQOU010000002        54923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_225_length_54923_cov_6.650069, whole genome shotgun sequence.
ACCESSION   DAOQOU010000002 DAOQOU010000000
VERSION     DAOQOU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 54923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54923
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225_length_54923_cov_6.650069"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /gene="glpK"
                     /locus_tag="PLM90_00170"
     CDS             complement(<1..1270)
                     /gene="glpK"
                     /locus_tag="PLM90_00170"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HMZ87806.1"
                     /translation="MQFILAIDQGTTSSRSILFDGEFRELAKSQQEFKQYFPQPGYVE
                     HDAEEIWTSQRETILNVLQRRDLNAVSAIGISNQRETVVVWDKHTGVPIHNAIVWQDR
                     RTAEICEQLKQQGHEQYIRENTGLVIDAYFSGTKIKWILDHVSGARDKANKGELLCGT
                     IDSWLIWKLTEGRVHATDVTNASRTMLFNIRSMQWDAKLLSFLDIPASMLPSVKMSHD
                     DFGYTILHGHQIPIRGVAGDQQAALFGQQCVQPGMVKNTYGTGCFMLMNIGEQFALSK
                     SGLLTTIAWGLDGKITYALEGSVFNAGAVIQWLRDSLQIISSSAESEDLARSIQGNDG
                     VYFVPAFTGLGAPHWDMYARGTITGLTRGTGKAHIARAALEAIAYQTRDVLDAMQRDT
                     NIPISSLRVDGGATSNNWLMQFQSDILQTIV"
     gene            complement(1342..1590)
                     /locus_tag="PLM90_00175"
     CDS             complement(1342..1590)
                     /locus_tag="PLM90_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87807.1"
                     /translation="MKNLYIITFALLTAACFFSCDDKTIACGEILIQVSEAAQDWNSS
                     TANCEAYIDAMHSYLDNDCATPTVVDEYEGILDTLSCP"
     gene            complement(1603..2028)
                     /locus_tag="PLM90_00180"
     CDS             complement(1603..2028)
                     /locus_tag="PLM90_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87808.1"
                     /translation="MDNKFLNTVRILFIGIFLAPLMFLGVILFTQSGQEKPLTVANDP
                     FFFIVVIMSVGAIIASTMLYKKRLPAIQEMTDTAAKLNAWKSLLIIKLAIIEGVTLFA
                     VVVLQIQLANIYLFIALALSAYQLTNIPTEEKIKTDCNL"
     gene            complement(2033..2860)
                     /locus_tag="PLM90_00185"
     CDS             complement(2033..2860)
                     /locus_tag="PLM90_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HMZ87809.1"
                     /translation="MNYQTYHWKQAAGQEIFAQSWLPDTTPKAIYCIIHGQSDHSSRF
                     THMAAYMVEKGFAVYAADLIGHGKSGGKRGHMLHFEEYLETVDALIDLALKASPGLPV
                     FLYGQSMGGNVVINHALRGTDKVKAYIASSPWIRLAFEPPAWKVGLGKMMKSIYPALS
                     QPTGLNADFLSHDKQVVSDYVNDPLVHGKITASAFFETLQAGLQIPDKASHLKTPMLI
                     LHGSEDKITSSIASKSFAEMRPDLITHKLFDGCYHELHNESVKEQVFETIVQFIEKQ"
     gene            2934..4007
                     /locus_tag="PLM90_00190"
     CDS             2934..4007
                     /locus_tag="PLM90_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ87810.1"
                     /translation="MKKFKPFLKVAAWIIGGIVLYLVGVILYGTITEYKPEPETSEVV
                     EIKGKATASEKMDSVLNLMTWNIGYGGLGKEMDFFYDGGKAVRAPREIWDKDFKGICA
                     TIESNDSIDFFLIQEIDRDSRRSYEVDMYREVNHILKGYSASFGLNYDVNFVPVPYTH
                     PMGKVYGGVATYSKYQPAEALRYQYPGHFPWPTRIFFLDRCFLEQRYPLANGKELVLL
                     NTHNSAYDESGEIKDGEMQYLRKFVDAEYAKGNYVIVGGDWNQCPPNFKNDKFKPKSG
                     YDGFMPPSMSFDYASEGWIWPYDPDAPTNRHLETPLDDKTFRTLIDFFLISPNVELLK
                     IKTINNGFDFSDHQPVVMQVRLR"
     gene            complement(4305..5840)
                     /locus_tag="PLM90_00195"
     CDS             complement(4305..5840)
                     /locus_tag="PLM90_00195"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HMZ87811.1"
                     /translation="MAIEQFQYIRSLQAYEGREVTIKGWVSNKRDSKGLVFLIMRDGS
                     GFAQCVVDAAKVSEEQFEAAKRLTMESSVCLTGTVVKDERQVGGYEIQVSAVEIIQIA
                     QEYPIAKKEHGVEFLVENRHLWLRSRKQWAMMRVRNRVIFAIHSFFQQNDFLQMDAPI
                     FTGNACEGTSTLFETDFYGRPAYLSQSGQLYGEALAMAHGKIYTFGPTFRAEKSKTRR
                     HLSEFWMIEPEMAFYNLDMNMDLIEQFLKYVVDDVITHCASELETLERNTAFLRNAVT
                     QKFPRIHYDDAVAMIRGEKEVDGKNSIKLLEADLEGIKLRIAEIAKEIAEREAKIEGG
                     SLKQGEINFNKNKIDTLKNESKELEERASNIPQWLESARNFQHGNDFGGSDETVLTRL
                     FSCPIMVYNWPAAVKAFYMKRDPNDPRYVKGVDVLAPEGYGEIVGGAEREDNYEMLLE
                     RIQHEQLPVEAFDWYLDLRKYGTVPHAGFGLGLERMVMWISGGQHIREAIPFPRFYGR
                     LEP"
     gene            6003..7487
                     /gene="rpoN"
                     /locus_tag="PLM90_00200"
     CDS             6003..7487
                     /gene="rpoN"
                     /locus_tag="PLM90_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HMZ87812.1"
                     /translation="MLNQRLSQKLLQKLSPQQIQLMKLLQVPTDQLEQRIKEELEANP
                     ALEEGDREEQDASSLEEVFREEEKGEEGTGEGEENRENEELDLDDYINEDDVADYKTR
                     DENYGDDDEDNKTTPYAVTDTFHENLSKQLGMCELDERQEKIAQQILGSIDDDGYLRR
                     EISAIVDDLAFSQNVMTEENEIIDLLDLIQGFDPPGVGARDLRECLLLQLERKTQTKA
                     IKLAIHIIENYFEEFTKKHYEKLARHMRVDEDDLKPVITEITRLNPKPGGSAKEDGGS
                     MYQYIIPDFTIMNNNGELELKLNSRNAPELRVSRDFMDMMVEYKRGQQKDKKQKEAIL
                     FIKQKIDAAKWFIDAIKQRQHTLWSTMYEIMVHQYDFFLTGDETQLNPMILKDIAEKT
                     GLDISTVSRVANSKYVQTEFGTYALKYFFSESLSTESGEEVSTREVKKILSELIEGED
                     KKNPLSDQELMEDLRKRGYNIARRTVAKYREQLDISVARLRKEL"
     gene            7484..8101
                     /locus_tag="PLM90_00205"
     CDS             7484..8101
                     /locus_tag="PLM90_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87813.1"
                     /translation="MIKKIFGHFFSILFLPLLLPLFGALFVIYSDPYGFPDQGANILM
                     LIRVGLLTFLFPAITIFLLVRLKFVQNVTVYDRQERTIPYIACGFFYIWAFYVFYKEG
                     FNSHITFILLGATIAVFIDFLINILVVKVSMHTTGAGGLVAFMLVLFPYAQINVLPVL
                     LASIVIAGCVGAARLALKAHSEREVYLGYLTGFFSFMVASNFIHL"
     gene            complement(8088..8894)
                     /locus_tag="PLM90_00210"
     CDS             complement(8088..8894)
                     /locus_tag="PLM90_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ87814.1"
                     /translation="MNKVFAIVILLASSLCLKANPDSTSTEGINNIDTTTFVYANVMY
                     EKGQYATSIDIYNRLLEQEGPSATLYYNIGNCYYKTGQLGEAVLNYERALFLDPGNDD
                     ATYNLSLANARIRDRMEPRTTSIFIIWWHAVSRIMGAQAWGIIAIVAMWLALMGWAAY
                     LLPQFRAYQRIGFFTAIIAFSISILCTIGHFGRKNYDAGQTFAIVMSPSAVIKSEPSE
                     TSTNLALLHEGFKLKLIEADSSWSKIEMPDGVIGWMHRKDYAAINPFTGE"
     gene            complement(8891..10714)
                     /locus_tag="PLM90_00215"
     CDS             complement(8891..10714)
                     /locus_tag="PLM90_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HMZ87815.1"
                     /translation="MACILPESLHAQVSFTATVNKNKVSVNERFQFIITLNNGSNPEN
                     FRPPSFTDFTVLGGPNQSTSVQMFNNTVQQSMSFSYILQPKKTGKFQIGAAYIKVKDK
                     TYSSQPIVIEVTDAPPATAGAQNNNSGSNNGSSGSASGDIEQYLRENLLIKTEISDQD
                     VYEGESFTVVLKLCIPDDGTIAGLNGYRITKTPAYDGFYVEDADLRNEQFKAENINGK
                     NYKTVIIKKTTLTPQQSGNLVIDPTSLDAGVVVRVKKQKKNSGDPFQDMLNDFFSDPF
                     SSGSREFTASLSSKTTGIKIKPLPANAPADFNGAVGKFTMSSQINSTSTKTDEPLTYR
                     ITINGTGNLQLFNAPELDLPAGWETYDPKVSESSGTKIFEYLLIPRSPGDFTIPAFTW
                     SYFDPSVKKYQTLQTDAYPVKVTAGPGYNPSANNYAANKEDVELLGKDIRYIDKDSPK
                     FSSGTKDMKSGFLLALILTPAIGGAGLFVFTRRQKAKMSDVGAMRAVKANATAKKRLA
                     AAGKFLQQNNSRGFYDETVRALWGYLSDKLHIPQNALSKENIEQVLLNKHVSASTIDQ
                     FIQLLDTCEFSLYAPNIQHSDLELTFNSASSLISTLENELR"
     gene            complement(10756..11451)
                     /locus_tag="PLM90_00220"
     CDS             complement(10756..11451)
                     /locus_tag="PLM90_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ87816.1"
                     /translation="MMKKNNIKHLLAIAFIFAVRTLTAQNANELIKEGNAKYDKQDYD
                     GARENYQEAVKASPESVEGNFNLGNSLYMQQQYDAAAEQFSKAAEGTKDPEIRSQAYH
                     NLGNTLLEKKDYSGSIDAYKKSLRDKPEDADTKYNLSYAQQMLKKQQQQQQQKQDQQK
                     QQQQQQQKQKQQQQDNNPQNNNQDKQPEPNTPDKKDLDRILKSLNEDDKAVQDKVNKQ
                     KARPAGGDPEKDW"
     gene            complement(11435..12484)
                     /locus_tag="PLM90_00225"
     CDS             complement(11435..12484)
                     /locus_tag="PLM90_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ87817.1"
                     /translation="MSFERPDILYLLILLPALLLMYWWYLRWRTARIKKIGDSELIRD
                     LMFRDSDKRRWIKYSLQLLGIAFLILGIANLRQGSKEQKVSGESAEVVICFDVSNSML
                     AEDVKPNRLTQAKFTASELIEKLAANKVGLVVFAGNSYVQMPLTRDARAAMMYLNTIN
                     TTSVPTQGTAIGSAIETAMLSFENGGEKDDKKGRAIIIITDGESHDDNAEEMAKKAAS
                     EGIKIITLGVGTSTGGPIPLRKNNNIEGFKKDRSGNIVLTKLNEQMLNDLATDADGVY
                     MNLAEGKRVIQEVYKTIDELDKTVDGDFEFTEYVNHFQLFLGIGLFLLILEFLMTDKK
                     PKWVEKIDLFDDEKK"
     gene            complement(12484..13491)
                     /locus_tag="PLM90_00230"
     CDS             complement(12484..13491)
                     /locus_tag="PLM90_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ87818.1"
                     /translation="MSFLSDIQFADPWFLLLLAGIPVYVYFRSKIERRKFVAMRMSTT
                     SGIPKAPQGKAKFMSVLLILRLLAFALIVIAMARPQTSYSNSRMYSNGIDIMLALDVS
                     PSMYAIDFKPNRMEAAKTAAKEFIDSRPNDRIGLVVFAGEAFTQCPSTLDHNLLKEQV
                     DNADNYYLKDGTAIGDGLFMAVNRLADSTNISQKVIILLTDGVRVGGKFSPLDAANGA
                     KELNMRVYTIGIGTNTNSPIPVFDKNGRHIFDLDPRISFDAPMLKNVASITGGSYFEA
                     DSKEKLTDIYQQIDKIEKTKFSMDISRRYDEHFYWFAVGGLICILLEVILSKTIFRTL
                     T"
     gene            complement(13488..14420)
                     /locus_tag="PLM90_00235"
     CDS             complement(13488..14420)
                     /locus_tag="PLM90_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87819.1"
                     /translation="MMKKLIVILLIIITCNQLQGQIISAHANTDKPSYSIGDYISVDI
                     TISGPASFKYYFPDETTISTYDIISSMPIDTQRSDDRVILTKHIIYSIYEGGDVYFPQ
                     VAINYQSTSDTAQHQILTDSIPLHIEAIAVDTTLAIKPIKSVLDVTLRNHKWILWVGI
                     ITGLIIIGGLIWYFFLRRKNIGPIAVPVPLKSLHERMTERLQQLQSKQLWQKGQLKPY
                     YSELTDILRDYLEERYHMHAHESTSDEIVEQLERHPDTRAHASNIRYLLNLADMAKFA
                     KSQPLADENTRAFDHVLNFIEQTREIITETPKQE"
     gene            complement(14421..15296)
                     /locus_tag="PLM90_00240"
     CDS             complement(14421..15296)
                     /locus_tag="PLM90_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ87820.1"
                     /translation="MDKATLLKKVRLIEIKTKGISQNIFSGEYHSAFKGRGMSFSEVR
                     EYAYGDDIRNIEWNVTARMNHPYIKIFEEERELTVMLLIDISASSLFGTQKQTKKALI
                     TEIAAVLSFSAINNNDKVGVILFSDRIEKFIPPKKGKSHILRIISDLLEVKAEGSGTD
                     IALALEYFNNVVKKKSIAFMFSDFFDMHYEKPLMVAARKHDVIGVHIHDKREEDLPDA
                     GIIRMFDPESRNIQWVDSSNKTVRASYKKYFNTNLQYFKNTFLRSGADTISIHSELSY
                     INALHAFFKKRISMR"
     gene            complement(15330..16331)
                     /locus_tag="PLM90_00245"
     CDS             complement(15330..16331)
                     /locus_tag="PLM90_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ87821.1"
                     /translation="MDAVISTDIRELNERIHQESAFIDLLRMETSKAIVGQKYMIDRL
                     LLGLLSQGHILLEGVPGLAKTLAIKSLSSAVNAKFSRIQFTPDLLPADLIGTMIYNQK
                     DNNFSVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGEHTYKLEEPFLVLA
                     TQNPIEQEGTYPLPEAQVDRFMLKVVIHYPEKEEEKMIIRQNVSGAIQQIKQVVKPED
                     ILKARDLVREVYMDEKIENYILDIVFATRDPEAYKLGKLKHLIAYGGSPRASINLALA
                     SKAYAFIKRRGYVIPEDVRAVCRDVMRHRIGLTYEAEAENITAEQIISELLNVVEVP"
     gene            16507..16905
                     /locus_tag="PLM90_00250"
     CDS             16507..16905
                     /locus_tag="PLM90_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ87822.1"
                     /translation="MTDFISIEATLGAENYKTSMHNGRSTVFCDEPADKGGKDSAPAP
                     RELLCMSLAACTAITLRMYAERKNWALGNIDVQVTLSEGEAGKQVFNRTIKPEHKQDQ
                     AIYDRLLMVADKCPVHKLLAGTNEIITTIA"
     gene            16905..17273
                     /locus_tag="PLM90_00255"
     CDS             16905..17273
                     /locus_tag="PLM90_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HMZ87823.1"
                     /translation="MQDITSRSDVAKIVVEFYNDVRRDELLAYIFNDVAQVNWETHTP
                     LIIDFWEYTVLGTGSYTRNAMTPHVALHAKSPLTQAHFDRWLQLFHATVDKYFAGENA
                     DLMKTRADGIAGLMHHKLNG"
     gene            complement(17281..17688)
                     /locus_tag="PLM90_00260"
     CDS             complement(17281..17688)
                     /locus_tag="PLM90_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMZ87824.1"
                     /translation="MKTSVIRRATFNAAHRLHNPAWDDATNSKVFGACNNPNYHGHNY
                     TLFVKITGEVDPVTGYVMDLKDVNILINTYVIEPLDHQNLNLDVPDFRNLNPTAENIA
                     RIIYEKLRSALAADLELKVTLYETENNIVEYPG"
     gene            17754..18242
                     /locus_tag="PLM90_00265"
     CDS             17754..18242
                     /locus_tag="PLM90_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ87825.1"
                     /translation="MQEKSSLHAFQFPSIDGGMIDFAVFKGKKVLVVNTASDCMYTDQ
                     YKQLQELSVTYPNELVVVGFPSNDFGEQEPGTNREIKNFCSYRYGVTFPLASKSPVIG
                     MDANPVFKWLTTVPFKTDMNKTVTWNFQKFLCDENGVLIEVYPPAADPINDGLLEHIN
                     PN"
     gene            18260..19438
                     /locus_tag="PLM90_00270"
     CDS             18260..19438
                     /locus_tag="PLM90_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87826.1"
                     /translation="MFFSQVIGQDRIKQQLIRAVEEGRVSHAQLFLGPEGCGNLAMAL
                     AFAQYINCENRQTGTDSNGKVTGDSCGKCPSCIKAQKVIHPDIHYTFPFVKIEDKELC
                     ADWMKEWREFVLQTPYGTYNDWIENITEGNKQGNITAKECLEILRKLSLKNYEGIYKV
                     QILWMPEFLDKEGNRLLKMLEEPPDNTVFLLVANDSEQILNTIMSRVQIVKFARLADE
                     DVAQVLERDHQQPHNQAVKIAQMAGGNLQEAIRLLDIHEDANTAMIREWMEACYRQQV
                     KQLFAWNDKFLKLGREQQKMFFQYCLHFFREVMLIQAGAGQLAKLTDAELKTAQGLGQ
                     VLGVEKLMGIIELFDKSIYFVERNVNGKIMMTYVSVTLLKQFQKNYQPTRPFYDRWAL
                     "
     gene            19450..20814
                     /gene="ricT"
                     /locus_tag="PLM90_00275"
     CDS             19450..20814
                     /gene="ricT"
                     /locus_tag="PLM90_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HMZ87827.1"
                     /translation="MSCAGCAVGTDKSSPAGCKSNGHCATGGCNKLNTFDWLRAMPVP
                     VSQRFNIHEVSFKNGVRKGFYANTKGIDVMTGDMVCVETESGYDVGRISLSGELVRLQ
                     MKKRRVSPNSTFSPILRKATDADMEKMMEGRALEGATMLRARTYARELKLDMKIGEVE
                     FQGDKRKATFYYIADDRVDFRELIKIYAREFRVKIEMRQIGARQEAGKIGGLGSCGRE
                     LCCSTWLTDFKSVSTHAARYQNLSINQSKLSGQCGRLKCCLNFELDTYMDALQDFPKH
                     ADILRTEFGDARLVKTDIFKKLMVYEYNAGGKQYPVTLEKVREVLSMNSKGQKPEHLE
                     GVKYEQEPAVKEVEYATVVEQVSLRTIEKAEQKRRQKNRQKGPQQQNQRRDNPPQGRR
                     DNNQNPRDQRQQEQKGNNQPRRDGNQQKGPGNQQQNQQRHRGNNRPPRGNNRGPGNDN
                     NNKS"
     gene            20817..21302
                     /locus_tag="PLM90_00280"
     CDS             20817..21302
                     /locus_tag="PLM90_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HMZ87828.1"
                     /translation="MNNRAYSILFSVLVTCFVAAGCEQSRVFDKNTPIDKSGWAYGTA
                     LSYEVQINDTTQSYNLYINVRHTDAYPYNNIWINMTTTFPDGTKQTTKVDVPLSEPEG
                     EWMGNCVDGICFNTTLIKSDFFLPQKGKYTFTLEQDMRMNPVPDILDVGIRVEKFMVT
                     R"
     gene            21374..23899
                     /locus_tag="PLM90_00285"
     CDS             21374..23899
                     /locus_tag="PLM90_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ87829.1"
                     /translation="MHRQTLIAILLSISFGIRAQAPTQPYIIMITMDDMNAYLGAYGT
                     HPQVETPNLDALLSMGTIFTNAHCASPKCAPSRTSVITGKDVDYTQVYDNTSYACRDF
                     RKNFKVAYGNAEVFTLPEYLKDNGNYYTYTMGKTLHCYEAYSDYDTVTLDPCEKQFSW
                     NNAFVYVEDEIIDPIGDAENEGIFLLKWARLNDTMIDYMEDDVTVDHAISFINDFSTF
                     GTSITCDRPFFLALGIKKPHAPFYVPESFFNENYLNSVYDDPYDIPYNVPYNANPYNG
                     IVMPPQPEPRWADYDSLGYMGQLFASDNEEGQFEAFGESLPSLPEIQAGISDSERIEI
                     LNESMRANALMAYLAGVKFLDYEIGRFLDALESHPEIYNNTVIIITADHGFSHGQKRH
                     WGKFSLWEPDMRVPLVYIDLRNPVAQTCNASVSNMDIFPSVLDMLDMAEPTFLSGDRY
                     LDGYSFMPLINNPNQPWERPVLAGVRTKNSGGTLNDGSCFTQYSLRNNRFHYIKYATN
                     NAPPLLTCDFAASQTEEELYDVGVNRETDPNEWNNLVDDPEYAPVLNYMRQFIKDSTH
                     YKDRMFAVNINNTNTLPCFVKNTGKIKLQAALYDLNGALLTGAGYTFTWTNSLTAATV
                     AGKNYTFNMATVPPAVYAAHDHIMFYLKVTNTTTGELVAFNTRTFYINNANTPNAVYS
                     LVTDNIALTASITGYTLTGSYNNTYWNFGDGTQSEDFLPDTHYYAAPGFYTVRNYIQY
                     GNGCLKNVARNANLTREGVPQLQFALYPNPAKQSVTIALSEAISDADITVYNMLGQPV
                     YMQSIKEADRSFTIDITDIANGTYMVEISTPQFYGTKTLEVIR"
     gene            complement(23903..24667)
                     /locus_tag="PLM90_00290"
     CDS             complement(23903..24667)
                     /locus_tag="PLM90_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMZ87830.1"
                     /translation="MKIITYNVNGIRAALTKGWAEWVQKEGFDIICLQELKAEPGQLD
                     LSIFDNLGYHHYWHPAQKKGYSGVAILSKVKPDHVEIGCGNSAYDAEGRVIRADFGDK
                     SVFSIYFPSGTTGDVRQQVKYQFLDYIYDYLIALNKKGRKLIVCGDYNIANKPIDIHD
                     PKGNKESSGFLPEERAWMDKFYENGFVDSFRQFNSEPHQYTWWSFRANARANNKGWRI
                     DYIAVSESLKKQLAGARIIPEAKHSDHCPQLLEVNW"
     gene            24899..27391
                     /locus_tag="PLM90_00295"
     CDS             24899..27391
                     /locus_tag="PLM90_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ87831.1"
                     /translation="MGWRTGSAQLDQPNFIFIVVDDLNDYVQGITDQPQVLTPNIQEL
                     ANRGMLFSNAYANSPGCAPSRTSFLSGKDIQYTGVYNNEDYASKFRENFSVANGNEIV
                     YSLPQILKDSAGYFTYGINKIFHNPNNNDFDKTIATPACDKTLSWNRMDFIEDSDSLL
                     EALSVYGFGNTFDWGMIPDSLEPLLEDYIGTNLAINFIDSVATGTAETCGAPFFMALG
                     YYKPHIERFIPEKYFPDYYLNDVYAEPFVLPYNNPVDAYPYNGIVMPVQPDLLYGDYY
                     NLPEGGIARAMADNGGVYDQIETYVTGLDPLPVIDDLLTDEDRMNILRTATAANYEIN
                     YIAAIQYIDAQLGRLMDALEAYPAIYDNTIVVLLSDNGYSLGEKRHWTKWTLWEPDLR
                     VPMIIADPSREGGEVVEQTVSLLDLFPTVCDFAHVNYPTFPDGSKYLDGHSIAMLLDE
                     PNLKYEYPSLSSYKKNGGIGSCYPHHSVRNERWHYIRYRENNDGTFATGFCDSINHSY
                     QQELYEIGIDRETDPYEWNNLAEDPDYAPVINFLEQWMPDSVYYLKKAFRVTMQHDAI
                     DCLLDPEGSVHLSASVFDTAGILFDLPDTMQLRWYTNKSPEYTYGQVVDIDLTTIPDG
                     AFDIDAELIIYVELIGADSNIIQGFDLQYFYADPIHAPEATYDVAYIDALTIAVTGYT
                     LTGTYYTSWWDFGDGTTSPATDPITHVYPDEGPYTLTNFVSYGNDTCVAAFSKEISFT
                     TDTSDPEIQLFCYPNPVDDLLRIYLPVQSAYTAVDVFDLAGRPVLSQRFITANTLLFI
                     SLDCSKLNNGMYIVCLNTDQGRYSAPVVVLHP"
     gene            27478..28506
                     /locus_tag="PLM90_00300"
     CDS             27478..28506
                     /locus_tag="PLM90_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ87832.1"
                     /translation="MKRILATLLLAGSVLVASAQDIHFSQYSQSPLTLNPALTGLTPC
                     TYRGVLNYRNQWASALGPASYQTFAGSFDAGLWRDKFDGNIVGVGAMMYNDVSGDGSL
                     TNMTVMGSFAYHQMIGDENNYLSIGAQLGLVQKRVDYSKLIFESQIGVNGVDPNLPSG
                     EYGDDNFSYLDVNAGINWRGRFSDKFAVQIGGAYFHLSEPIETFYNYNVNKLNAHYVG
                     YGSFKIGMGKSTVLIPSFLYMQQTEGSNVQINSGVDVGFQLEKGVSMAYAGLHYRSVD
                     NIDVADAAIINLGLDYNNINFGLSYDVNISGLNAVTNYRGGIELSFVYWGCLSVTPKS
                     KPIDCPRF"
     gene            complement(28507..29247)
                     /locus_tag="PLM90_00305"
     CDS             complement(28507..29247)
                     /locus_tag="PLM90_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMZ87833.1"
                     /translation="MDASATVFDNIVQSRRSMRIYDAEAYFDSEAVARSIRRAMLAPN
                     SSNMQMWEFYRVSDPQKIKDIAVFCMNQNAAKTARELLIIAVPKNKYRARAQANYAFH
                     HARYGNKPIEELDKRSARKLKYFSRLMPMYYFQDWLGLWGAVRKLIVSIASIWRPVVW
                     QVSKQDLRVVCHKSAALAAQTFMLSMLAEGYATCPMEGFDSKRLRKYLKLPRSTEFNM
                     IISCGPGTTQGIYHERFRVPENEVVFMV"
     gene            complement(29295..31271)
                     /locus_tag="PLM90_00310"
     CDS             complement(29295..31271)
                     /locus_tag="PLM90_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HMZ87834.1"
                     /translation="MLLILFQNHTHAQVNSSFELYRSNIAAAQANMQLHQFTEARNWL
                     NAAPEKYRAWEWNYLYYTLDQSIGKIELGYIANDMAISPDGKSIAIAGADHNIHIYDV
                     LSKSETSTITTHQGKVYSIAFDPSSQIIYSSGQDAKVIATKITDKSKVWEKSIPAGIN
                     KLSVSRDGKLICVSTTLFNGYGYTGNTIVLQAQTGSDIWKSGERKEKPLASAFSNDGS
                     MLAYGEENGVISVIRTDSFTVVQNYIFDDNNSIQQIKDITFSEDGSFLMAANGNGFAQ
                     VWNMFTRTTQQKLYSAQQNITTVGYTPNGFGMVTGASDGSIALWSTESATVKQELFGH
                     IGEVKKLLTTQNGDVLVSLSADGTLRFWDLAKAGTYINPMGFSGLTYAFEVSADSKYL
                     FTQGEFGLITQWNNATGYIEKQFPSLPEIMNDITIDHDNHLIAACDNEGHVRAWETAT
                     GKRYDDYFGVEGAVYDLDYNPDGTLLAAASADSNITIWNAVSGGIFQQIHFAGQVMDL
                     SFSYNGKMIAGVSSLGIIGVWNMETKEFVFNTTISKNHLQKVLFSPNGQNIIATDDNG
                     TIYILAADSGKLLKQLNAHTGIVYDVSFSVDGKRLASGGGDGTIKIWDTETWESVLSF
                     NDMHVWIHNVQFSSDGNHLYINAPTKDFKRYSIK"
     gene            complement(31368..33836)
                     /locus_tag="PLM90_00315"
     CDS             complement(31368..33836)
                     /locus_tag="PLM90_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ87835.1"
                     /translation="MKKVILLLLFCQGVMLYAQQMQPKQNDYVSITNGKTKIPSKHIT
                     QELINNTPEAFQSHPDFGIPPVNAPKGAIELVQKRTLDSRYYIKEGTNGQSFFKQQAY
                     GAINYTDAQGYLRAIDYLLLPTINEGVFAAASQPTPTSIDFINNFTSIHIRDLDFRMN
                     HDMEMYFEKGNTTTAKQRYNTSNYTVGRDGTWITDIFSGVDGEIAFRKAGIKTNFIIK
                     SKEVVDPDAEWLVIDDHIALPPGYQFVYEDGDGYFLSNGDWKGNVVVKNPFGLNLLRI
                     ERPVILDQNKSKYHKQDQVDAIAYQLIPEDGGYILRMRVNVKWLINSERKYPVVIDPT
                     LIGEATYTAGDIGFEYDALCWNEADYCSYSLDITVPGKTTLTAAYFDGTYYSQNFGCF
                     FVTDCLMSEAAFRILGICDDSPGPASFWTCLPPVGDSAGTCYGIDLPMFNTIACIPPQ
                     CADYNFTFEMRTYHCSCTQPPCSILCHFMPTGSWVITIEGKTVEENPIQSSTVPDFVI
                     CEGDSIDLFASGVWGVPPYTYEWLPGGVVEPIHWVAPTTTTTYTSVIHDACDMTDTVS
                     QTVTVNPAPIIEVGPFEGCYLTTMTAPGGYDSYVWTDANDSVLATGTNTLTVDSTGMY
                     FVTVTDGNGCMGTSEPIEAFVYEAPVINAFPDTVFVNDGALALLQVETITGDDVTYTW
                     TPADAVNCLNCATTLAYSVGESNTFYVTGEEHGCVSAPDSIVVIMEESELIIPNAFTP
                     NDDGLNDEFNILNPIFYPIFSFEIYNRWGQQVFATSDVTRGWDGNYDGKPQEIGMYVW
                     MVSYEKANQPGEVFVLRGTVTLLR"
     gene            complement(33867..36788)
                     /locus_tag="PLM90_00320"
     CDS             complement(33867..36788)
                     /locus_tag="PLM90_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ87836.1"
                     /translation="MTKLYTLIVGLIMSATLAGQTFQLFTEDFNVPTGSFILNTPGPG
                     GAAVGTNKWVINNSYTGGFGYPNTTTQDLTESGTIGGAPTSTYLHIYDEEGAPAITCA
                     SYDPTSTSDNFAEMSGGFCTLGLIDIEFTFFWLCEGGAGDYGQVYYSADGGPWTPVGM
                     ALYSGQTLWKYEVITDPAFEEVANLRFAFRWVNDNGGGPSTMSFAVDDIIAVGTYDEA
                     IHPVDINIDFISPDPVCKLSTLIIGWSLTDPLCDGIYEIELSNAAGIFAAPTSLGVFT
                     IFAEDTIGAIAAVIPGGVPDGSCYKVRLSRTSPLPEITGEASECFTIQSCPNIITTLQ
                     PVVTYDSNAVCVNSVIDVPFFSTGVYFPGNVYTAQLSDSTGSFDTYSVIGTFASNATY
                     DPALGSPPGSVSGIVPMVPPGCNYYIRVVSSTPASIGSAYGTVCIQECDIETNDIEDV
                     YACITEDSGVTITITYDVNVFDDIETYCDTNTFCVEVLNSMFFTQANLCGLGFTIDTE
                     SGTIDLEIPGYFDLVAMGLDAGMWYMRVIANCGTPQENTLGTLVHLTIGAPADNAPVL
                     IPSDTLLCEGAVGNATIVPYNMNSDYQFQFGAGTPFIWPFNPIFIDFTGATGDVTLRV
                     REINYNCPGPWSEYVTFHIIDAPIVTISGPPKACTGDTTWYSVPYFLTTYYDWSLTGG
                     TIVDTSNNVVGIIWDEAGTYTLSIFALNECGSGNGTKNINVIETVPVEQSEDKTICEG
                     SSATFSVLTVGVPYYEWSDAETGEVIGDDFIITVTPDSTSTYVVTAEDEEGCNSYDTV
                     TVFVEYPSTESDTASYCIGGSVIIDAGHEGATYLWNTGETTKTIEVNAAGTYIVTINA
                     PDLACDITKSIDVSEVIDNCAPIVNIPNAFSPNGDGVNDFLTIYGAAVIDINIHIYNR
                     WGELVYTTNDLGVLNSGSLGWDGKANGQDQEMGTYVYVVSATGGNGNTITLQGNVTLV
                     R"
     gene            36915..38402
                     /locus_tag="PLM90_00325"
     CDS             36915..38402
                     /locus_tag="PLM90_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMZ87837.1"
                     /translation="MRALNRLATQTAIYGMSTILVRLLNYVLAPLHTRVFTNQADYGI
                     ISEMYAYVTFLNVVCMFGLETAFFRFASNNNQNSLDKRSVFGSSQISLIVSTALITAA
                     LLFSSNDIASMLGYPGKGIYVTFFALIIAFDTLVNIPFARLRLEGRPWRYFAIKLTNI
                     IINVSFNFFFLLPALRNNYDMFSGIGYTYDPDYGVAYVFLANLIASAITFAIFIPGML
                     SLKFDVQVWKKLMAYGLPLIIIGLAGMINETLDRVLIKYWGSGTIEENLKQVGIYSAV
                     YKLSIFMTLAVQGFRMGAEPFFFSQSAEKNAPSLYALIMRYFVIACCLIFLGVGMFPD
                     LFKIIIGSEYHSGLHIVPILLLAQLFLGIYYNQSVWYKLTDKTSFATIIPVAGALITL
                     GMNLILIPKFGYTGAAWSTFACYLSMVVMSHIIGQKHYHVPYNLRKIITYIVVSVILC
                     YSGLLLMSATENIVVTIVVRSCLFGLFVAFAWWLDLSKLLRRNKS"
     gene            38402..38839
                     /gene="dut"
                     /locus_tag="PLM90_00330"
     CDS             38402..38839
                     /gene="dut"
                     /locus_tag="PLM90_00330"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HMZ87838.1"
                     /translation="MLFMLIKIVNTSNNPDPTYATAHAAGMDIRAFVDAPVTILPMQR
                     TLIPTGLFIELPEGYEAQIRPRSGLAINSGITMLNTPGTIDADYRGEIKVIVINLGDA
                     PFIVNSGDRIAQMVIAKYESVTWVNTDKLSESERGAGGFGHTG"
     gene            38912..39916
                     /locus_tag="PLM90_00335"
     CDS             38912..39916
                     /locus_tag="PLM90_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ87839.1"
                     /translation="MNIIIPMAGMGKRMRPHTLTVPKPLIPVAGKPIVQRLVEDIAHT
                     TDEKIDNIAFVVGRFGKEVEDMLVGIAAKLGAKGHICYQDEPLGTAHAIMCAKDYVTG
                     PTIIAFADTLFKTNLKIDTTIDGIIWVNKVENWKMFGVVEMNADGYITNFVEKPDHYV
                     SDLAIIGVYYVKDGDLLRNEMQYLLDNNIREKGEYQLTNALESMKQKGYRFSPGKVDE
                     WLDCGNKDATVYTNQRVLELKKDAETLISPSAKLINSVVIEPCFIGEGAVIENAVIGP
                     HASIGKNAVVKNALIRNSIVQQSVSITDKCIANSMVGSFAVISGTPDDLSVSDYSTMA
                     "
     gene            39913..41682
                     /locus_tag="PLM90_00340"
     CDS             39913..41682
                     /locus_tag="PLM90_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ87840.1"
                     /translation="MRKKLRLQIGLCALTLMFLAACNGPKEAQQQTGSVVNVGSSASR
                     PKKGIDTAELERQYIDACKFVALEDYNKAVDIFNNIVKIDPNNAPAMYQLGKIFANHG
                     QIGDALAYSEKAASIDPGNRDYQQQYAGILQYSGQGRKAVDVYLGMIKNEIGDEDTYY
                     QLAATYEKLGDRQQAVSTLLDLHKIIGDDEQVMFEIQRLYAAVEQYNNAISWLQKLIK
                     QNPDNTLYLRYLSDYYDRNGQPELAEKTFNDLLAIDPSNTDFQFRKASMQQKAGEYAD
                     YVNTMNNAFANPDGNIDTKIFYLVLFIDSIGRPEFKTKDLVFNWSKLLVEAHPDDAKS
                     YAMRGDFLYYDEQLQSAANVYNKSIALRNDVYDVWLKLFYIYSDLHKSDSLALVSNMA
                     IELYPNQPLSYYFAGIAANQQDKFEDAVKVLRRGLPLTISNIDLRSGMFSALGDAYNE
                     LKKYPESDDSFENALNLNPNDVYALNNYAYYLSLRKEKLERAAELAKKATTLQPENPS
                     FLDTYGWVLYQQGDYKNAAAWIEKAMQKGGDKSGTVIEHYGDIQYRLGNEAKAMEYWK
                     KAKAAGDASDMLDKKINDNKLYE"
     gene            41675..42427
                     /locus_tag="PLM90_00345"
     CDS             41675..42427
                     /locus_tag="PLM90_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HMZ87841.1"
                     /translation="MNKNAYFILLIATFSLSACGTSHALFSGKIKPMTAEELDKELRS
                     QLIQFETFSSKAKIDLQSNQGNQSFSASIDIRRDSLIGISLRFLGVEGARIRITPDSI
                     EILDRLNQEYLPRDYTFLRDSLNLDLNFSDLQNLIIGNPVMYDSTTLETVESEEFYIL
                     NASKGVYKNQLSMAADFSIVRMFIQDLYARRNLTLLYAEYDKVNGKRFALNRNIKLDA
                     REDLTADVVFTNVEFDAPFEFSFSVNSKYKVN"
     gene            42462..43778
                     /locus_tag="PLM90_00350"
     CDS             42462..43778
                     /locus_tag="PLM90_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ87842.1"
                     /translation="MVCIVLSVSLSLQAQTQDKNALQNKYNKLQDEIKDAEELLDNTK
                     QKKENSLNELKLLNRKIDMREQVINNIAAQVDGVNAELTTTNKAITAMQADIVELKSQ
                     YAKMVYYTYVNDQDYKPLHFIFSAESINDAFQRVHYVRSFHGYRKDQIGAIEEISESL
                     QNKIAQIEKEKNEKESLLTKEEQQKKKLDDEKAKKDQAVKKLQTQEKDLKKQIEKKKK
                     DAVGLKNKIQAIIAEEIKKEKERAAQEAAKQKELEAKKLAESNKTGTTPTTNSGSTSN
                     AKTTNAEKPAAEKVNLGLTPEMQLISKNFEGNKGKLPWPVERGTITERFGKHEHPVLK
                     DVIVENNGIDIATAEGATVRSIFDGEVVNVIFNPSFQKGVIIKHGEFYSVYTNLSDVT
                     VKAGDKVSTKQKIGIAWEDPEEGKTEVHLEIWKNTVILDPSLWISK"
     gene            43895..45436
                     /locus_tag="PLM90_00355"
     CDS             43895..45436
                     /locus_tag="PLM90_00355"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMZ87843.1"
                     /translation="MIANDAEKFKKVVSHAKEYGFVFPSSEIYDGLSAVYDYGPYGAL
                     LKNNIKEYWWKAMVQMHENIVGLDAAILMHPTTWKASGHVDAFSDPLIDNKDSKRRYR
                     ADNLIEEYLAKQDDKINKEVEKARKRFENFDEAMFRSTNGRVLEITQKREEVHARFVK
                     AMQDGDLTEMKQIIIDCDIVDPESGSRNWTDVRQFNLMFSTKLGSLAEDADIIYMRPE
                     TAQGIFVNFLNVQKTTRQKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEELKVYDYWKQTRMKWHRALGTDAAMLRFHDHDKLAHYANAAVDIEFNFPFGFKELE
                     GIHSRTDYDLGRHSEFSGKKMQYFDSELNESYVPYVVETSIGCDRMFLAMLCNAYDEE
                     QVNDGMRSVLRLPAALAPVKVAVLPLMKKDGLPEKALEIFNDLRYRHQCYYEEKDSIG
                     KRYRRMDAIGTPYCITIDHQTLTDNTVTIRERDSMEQVRIHIEDVAKVVSEKVDMNNV
                     LRTLV"
     gene            45576..45968
                     /locus_tag="PLM90_00360"
     CDS             45576..45968
                     /locus_tag="PLM90_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87844.1"
                     /translation="MKRCLPVMLLSCMLAVVSCDEEPIYEDLNGSENLNRTIKFDVSH
                     VYATSPYSDSTVAGATIKIYAQFEDLLTDYYPDASRITDSLGHAEINGLDEDMYYIKC
                     THPTLGELIDSVTTPAYTTSFVELIYFM"
     gene            complement(46021..46611)
                     /locus_tag="PLM90_00365"
     CDS             complement(46021..46611)
                     /locus_tag="PLM90_00365"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HMZ87845.1"
                     /translation="MITTGKFATILLAGSLFLGACENSSQDAIKEKRKEEMTNKENRV
                     SPPATATANIGENTVTINYSSPSVKGRQIWNALVPYNEVWRTGANEATTISFTQDVTI
                     LGQPLAKDTYSLFTIPGEGEWVVIFNFNETQWGAFKYDQSEDALRVNVKPTMTETLTE
                     SLKFEVIAGENAGSGIIRLNWEKLQLDIPFENVATK"
     gene            complement(46836..47675)
                     /locus_tag="PLM90_00370"
     CDS             complement(46836..47675)
                     /locus_tag="PLM90_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HMZ87846.1"
                     /translation="MQNDQSIKDMVKDRYTEIATQSKSTNASSCCGAGGCSTVDLQIM
                     SEDYTSLDGYLADADLGLGCGIPTQFAQIKAGDTVVDLGSGAGNDAFVARSIVGEDGR
                     VIGIDMTQVMVDLARNNAQKLNLSNVEFRLGDIENMPLTANKADVVISNCVLNLVPNK
                     EKAFQEMYRVIKTGGHFSVSDIVIVGQLPTELLQEAEMYAGCIAGAIQKDTYLDIIHK
                     AGFSDVRIMKEKAIDLPDDILSNYLSADQIASWRNSGTGIYSITVFGVKAKTCGCGDN
                     CCS"
     gene            complement(47686..48120)
                     /gene="arsN2"
                     /locus_tag="PLM90_00375"
     CDS             complement(47686..48120)
                     /gene="arsN2"
                     /locus_tag="PLM90_00375"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HMZ87847.1"
                     /translation="MQISKAHSTDFANIHALLKANDLPVEGVNEVQGAYYLLKNGEKL
                     MAVAGIEGSAPTALLRSVAVDVQMHNRGIGTQFMIDILSLANEQGFRQIYLLTETASK
                     FYERLGFTIEDRSQAPESIRKSSEFTSMCPTSATLMKYIIIK"
     gene            complement(48120..48434)
                     /locus_tag="PLM90_00380"
     CDS             complement(48120..48434)
                     /locus_tag="PLM90_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ87848.1"
                     /translation="MAYTKRTEFSEDEMQLADIAKALSHPARIAILKVLSDGQCICGD
                     IVDRLPLAQSTVSQHLKELKSAGLIKGEIDGPRVCYCLNEPVIGKARKHFNFLFTSFN
                     CC"
     gene            48557..50368
                     /locus_tag="PLM90_00385"
     CDS             48557..50368
                     /locus_tag="PLM90_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87849.1"
                     /translation="MKHYLIATLCLTFATQSVSARILGGELWYEQTAADQYIIKLTWY
                     REYEPELEPVFWLGIYNNTDYTLIDTVAAIEIDRSIVDSNYPTCVGMVQKYDIERLDF
                     STPVTLPENPDGYVIVIMGNTRTEEISNILLPGLRGLTLYTTVPGNVNNSAPSFNNPP
                     VWYACEDQSLNMDLGASELDGDDLSYALVPALNADYTEPFFQSLPTPPPFSELSYLGA
                     LSPTNPFPSATTIEIDPSTGMLTGLPTTEGVYIITVEVTETRLGIELSKTRRDIILIV
                     EDCTPTMAEVDLGSEIYQCNDTHVFFECETEPWAGYYWDFGDGIIQTWDIYEHTYPAL
                     GDYELMYIANYDLPCADTFYLPVHIRELSEDVLSTDLLGNPENCFYDTIQLIGEPGFV
                     AEWQDVNTGLIIGTGSPLPVTQFSGDLLATAYYYDDDFCFASDTVSAYFFPDNTPLVK
                     INQDNPQWFLYCDNAFDPFVETLQWFKDDMPIPGAINPNLPLTSCGSYYARITDTHAC
                     VFESEATYYCNSEIEGAETLFFEVSPNPSSGSFSLTLPNTDPVTIRIYNNLGEKVYQI
                     ESAGNAVININTESLAGLYHVIIAGETYVKELPVMIN"
     gene            complement(50361..52307)
                     /locus_tag="PLM90_00390"
     CDS             complement(50361..52307)
                     /locus_tag="PLM90_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ87850.1"
                     /translation="MIAVQNLSISFGGEFLFEDVSFTLKDNDRVGLVGKNGAGKSTLM
                     KILSGLQQPDSGQVILPKDSLIGFLHQDLAKMKGKTVMEEARTAFDEALAFDKRLHEI
                     EHDLTVRTDYESDSYHDLIEEMNEIVHRLEIIDVGSIDKQMELVLMGLGFMREDFNKP
                     VETFSGGWQMRIELAKILLSKPELVLLDEPTNHLDIESIQWLETYLETYPGAIILVSH
                     DRTFLDNVTKRTIEIVSQKIYDYNAAYSTFVELRKERIDQQKAAQKNQEKYVEHTERL
                     IEKFRAKANKAKFAQSLIKKLDKLEEVEVDDEETATIHFYFPKPPRSGRISVEGRGVS
                     KSYGEKEVLRLLDFEIHRGDKIAFVGKNGEGKSTLSKMIAGIEKYTGELTIGHNVHLG
                     YYAQNQAETLSGDDTVFDTIDKVAAGEIRSHIRTLLGAFLFGGEASFKKVKVLSGGEK
                     SRLAMCKLLLEPYNLLVLDEPTNHLDMRSKDVLKNALANFDGTVIVVSHDRDFLKGIT
                     NRVFEFKDKTIKEYIGDIYDYLDAKKISNLAELEMTEKQPKKKEAVPEVVQEQKVQYA
                     DKKQKEKELQRVKKAIEKSEQEIKTLEAEIKTMEATMNSADFYNNSNTADFFTKYNTT
                     KQSHESKMSDWEKLQEELERLNVN"
     gene            complement(52332..54620)
                     /locus_tag="PLM90_00395"
     CDS             complement(52332..54620)
                     /locus_tag="PLM90_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87851.1"
                     /translation="MNCKRVILKAVFSVITISSSMLRTGMAQDEFHAEGVKTYNYGTF
                     NTAVSDTPKELFSLTPDSLYTHPEFGILPYNAPCEDCIELLQYRTQNSRMFVKKGTNG
                     SKFYSQGAYQDLNYKDNKGNWVTIDPTLQPSTALPGVYLGLHQTNPTTLNMVEGYTAI
                     AMQDGAELRFNQNISAYATSEIGLNIPGIAINRSVYSVGADGAAVKDAFPGIDQELRF
                     NVAEIKSGYTVWNLNAIDTSKKYFVIRDEFDLPEGYTISTDQYEGTFNVNGFWFGDLV
                     IENADGFEMARMKAPVMYDSNPNDTLDFVIPMDIIGYTVKQNGRTVTLKLVADMAYLR
                     DPQRQFPITIDPTVYGTTTTWTGTSGTDDSPTFCNVVVNVPTPANATLTGSSVHWEFD
                     ADGVACSPFCKLKFLQLRIGTSCGYSPSAAGVWVCPACNTAGTWNPTVDDATTAALVS
                     CFTPQCASFNIAFTIYHNQYQCVTPGGCVTTCAHLDLATITVEGETVTTTGLAAGATA
                     YTVLDCTLQSGWLSTTTPNYGVPPYTYTWSPGGYTTSPVYVTFPMGTTTYTVTMTDAC
                     GNVATDNVTVINNCVTLPIELISFSGYSLQDVNILEWEASSSLENKTFFVERSSNGRD
                     YVSIGMVEAANPWQTKYSFSDDQIASNVYYYRLKQVDEDGTTDYSNIISIQSSGNTDA
                     LVINEYDANTGNLALTLFSPYTGSLTLHVFDITGRLIADYPFQTQAGATSIQLQLPSI
                     AAGTYLLSIQETGVNASARLVR"
     gene            54707..>54923
                     /locus_tag="PLM90_00400"
     CDS             54707..>54923
                     /locus_tag="PLM90_00400"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HMZ87852.1"
                     /translation="MSQIQKSTLKFLKDLAAHNDRVWFEDNRTIYEAAKGNFIDFTAA
                     VLEKTASFEPAMAGIDAKKAVFRIYRDV"
BASE COUNT        15814 a        11408 c        11282 g        16419 t
ORIGIN      
        1 taacaattgt ttgcaaaata tcactctgaa attgcattaa ccaattgttg gaagttgcgc
       61 cgccatcaac gcgtaatgat gaaataggaa tattggtgtc gcgttgcatg gcatccaaca
      121 catctctcgt ttgatatgca atagcctcca acgccgctct cgcaatatgc gctttgcctg
      181 tgccacgtgt tagtcctgta attgtgcctc gtgcatacat gtcccaatgt ggcgcaccta
      241 atcctgtaaa tgcaggaaca aagtagactc catcatttcc ctgaatcgac ctggcaagat
      301 cttctgattc agcactactc gaaataattt gcaagctatc tcgcaaccac tgaataacag
      361 cgccggcatt aaaaacactt ccttctaaag cataggtaat tttgccatca agtccccatg
      421 ctatggtggt gagcaatccg cttttactta aagcaaattg ttcgccaata ttcatcaaca
      481 taaaacaacc cgtgccataa gtattcttca ccatacccgg ttgcacacat tgctgaccaa
      541 ataatgcagc ctgctgatct cctgctacgc cgcgaatagg aatttgatgt ccgtgcaaaa
      601 tcgtataccc aaaatcatca tgcgacattt ttactgacgg caacatcgaa gctgggatat
      661 ccaaaaaaga taacaatttt gcatcccatt gcatgctgcg aatattgaac aacatcgtcc
      721 gcgatgcatt ggtaacatca gttgcatgca cacggccttc ggtgagtttc caaataagcc
      781 aactatcgat agtcccgcat aacaactcgc ctttattcgc cttgtctctc gcccctgata
      841 catgatcgag gatccacttg attttagttc cggagaaata tgcatcaata accaatcctg
      901 tattttcacg gatatattgt tcgtgtccct gttgctttaa ttgctcgcat atctctgccg
      961 tacggcgatc ctgccatact attgcattgt gaataggaac tccggtgtgt ttatcccaaa
     1021 ctactactgt ctcgcgttga tttgaaatgc caatcgctga aactgcattc aaatctcttc
     1081 gctgtaatac atttaaaatt gtttcccgct gactcgtcca tatttcctct gcatcatgtt
     1141 caacatatcc cggctgagga aaatattgtt tgaattcctg ttgcgactta gcaagttcac
     1201 gaaattctcc atcaaacaaa atacttcgtg agctcgtagt tccctgatca attgctaata
     1261 tgaattgcat ttctttgcgg ctttattcct ctgcgtcttt gcgcctctgc gtgaaaattg
     1321 ggcgagcgag tgtgaataat gtcaaggaca actcaacgta tccaatatcc cctcatactc
     1381 atcaaccacc gttggcgttg cacaatcatt gtcaaggtag ctatgcatag catcgatgta
     1441 ggcttcgcaa ttggcagttg atgaattcca atcctgtgct gcttcgctca cttgtataag
     1501 gatttctcca catgctattg ttttgtcatc gcaggaaaag aaacacgctg ccgttaataa
     1561 tgcgaaggtg atgatgtata gatttttcat gttgaagttt tattaaaggt tacagtctgt
     1621 ttttattttt tcttccgttg gaatatttgt aagttgatat gcgctcaatg ctaatgctat
     1681 aaataagtaa atatttgcca attgtatctg aagcacaaca acagcaaata gtgtaacacc
     1741 ttcgataatg gcaagtttaa taatcagcag cgacttccat gcatttaact tggctgcggt
     1801 gtcagtcatt tcctgtattg caggtagtct tttcttgtat agcatggtag aagctataat
     1861 ggcgccaacc gacatgatga ccacaataaa gaaaaatgga tcatttgcta cagtaagcgg
     1921 tttttcttgt cccgattgcg taaataaaat gacacctaaa aacattaatg gcgccaaaaa
     1981 tattccgata aacaaaattc ttaccgtatt tagaaattta ttatccatgt aattattgct
     2041 tttctataaa ttgaacaatc gtttcaaaaa cttgctcttt aacagattca ttatgtaatt
     2101 catgataaca accatcaaaa agcttatgtg taatcaggtc cggacgcatt tcagcaaatg
     2161 atttggaggc aatagaactc gtaattttat cctccgaacc atgcaaaatg agcattggtg
     2221 tttttaaatg tgatgcctta tcaggaattt gtaaacctgc ttgcaaagtt tcaaaaaatg
     2281 cactggctgt tatttttcca tgcaccaagg gatcgttgac atagtcagaa actacttgct
     2341 tatcgtgcga aaggaaatcg gcatttaatc ctgttggttg cgataaggcc ggataaattg
     2401 atttcatcat ttttcccaat cccaccttcc acgccggcgg ttcaaatgcc agtcggatcc
     2461 aaggcgaaga tgcgatatat gctttcacct tgtctgtgcc tcgaagtgca tgattgataa
     2521 caacatttcc tcccatactt tggccataca gaaataccgg aagcccagga cttgccttta
     2581 atgctaaatc tatcaaagcg tcaactgttt ctagatactc ctcgaaatgc agcatatgtc
     2641 cgcgtttgcc gcctgattta ccatgcccta tcaggtctgc agcatacacg gcaaaacctt
     2701 tttccaccat ataagcagcc atatgcgtga acctcgaact gtgatcactt tgcccatgga
     2761 ttatgcaata aatggccttt ggagtagtgt cgggaagcca gctttgggca aaaatttcct
     2821 ggcctgcagc ctgcttccaa tgataggtct gataattcat aacggtcgaa aatacgaatt
     2881 aaatcgaagt taagtgccga ccaacatatt gcacggaggc gtatcttcac accatgaaaa
     2941 agtttaaacc ctttttgaaa gtcgctgcct ggatcatcgg aggcattgtt ttatacttgg
     3001 ttggcgttat cctgtatggc accatcactg aatataaacc cgaaccggaa acttcagaag
     3061 ttgtggaaat taaaggtaaa gcaaccgctt ctgagaagat ggattcagta ttaaatctga
     3121 tgacatggaa tatcggatat ggcggactgg gaaaagaaat ggattttttc tatgatggcg
     3181 gcaaggcagt gcgtgcacct cgtgaaattt gggataaaga ttttaaaggt atttgtgcaa
     3241 ccatagagtc gaacgatagt atcgatttct ttttgattca ggagatagat cgcgattctc
     3301 gaagaagtta tgaggtagat atgtatcgcg aggtcaatca tatcctgaaa ggatatagtg
     3361 catcattcgg attaaattac gatgtcaact ttgtacctgt tccatataca catcctatgg
     3421 gaaaagtgta tggcggagtt gctacctaca gtaagtatca acctgctgaa gcattacgct
     3481 atcaatatcc cggacatttt ccatggccga caagaatttt ctttctcgac agatgttttt
     3541 tggaacaacg ctatccatta gccaatggga aagaattagt attattgaat acacataact
     3601 cggcatacga tgaatccgga gaaattaaag atggtgaaat gcaatatctc agaaaatttg
     3661 tggatgctga atatgcaaaa ggtaattatg taattgttgg aggcgattgg aatcagtgtc
     3721 cgccgaattt caaaaatgac aaattcaaac caaaatcggg ttatgatgga tttatgccgc
     3781 caagtatgtc attcgattat gcctcggaag gttggatctg gccttatgat ccggatgctc
     3841 caacaaaccg acatctggaa acacctttgg atgataaaac tttcagaaca ctgatagact
     3901 tctttttaat ttcaccgaat gttgaactgc ttaaaattaa aaccatcaac aacggatttg
     3961 atttctccga tcatcaaccg gttgtgatgc aggtgaggtt gaggtgagtg ggtgagtggg
     4021 tcagtgggtg agtgagcttg cctcgccatt ggcgggtggg tcagtgggtg aatgagaata
     4081 aaaaggatta atcaataaag aataagtaac ttatccttca aatcttcaaa ccttcaaatc
     4141 ttcaaacctt cgaatcttca agccttcgaa tcttcaagcc ttcaatcctt caaaccttca
     4201 aatcttcaaa ccttcaaacc ttcaaatctt caaatcttca aaccttcgaa tcttcaaatc
     4261 tttaaacctt cgaatcttca attcttcaca cctcaacccc ttcatcacgg ctccaaacgt
     4321 ccgtagaaac gtgggaatgg aatagcttcg cgaatatgct gaccacctga gatccacatc
     4381 accatacgct caagtcctaa tccgaaacct gcatgcggca cagtgccata tttacggaga
     4441 tctaaatacc aatcgaatgc ttctacagga agttgctcat gttgaatgcg ttccaataac
     4501 atctcgtaat tatcttcgcg ctcggcacca cctacaattt cgccatagcc ttccggtgcc
     4561 agcacgtcga caccttttac ataacgcgga tcgttagggt cgcgcttcat atagaaagct
     4621 tttaccgcag caggccagtt gtaaaccatg ataggacaag agaacaggcg cgtgagtaca
     4681 gtttcatcac ttccaccaaa atcattgccg tgctgaaaat tgcgtgccga ttccaaccat
     4741 tgaggaatat tacttgcgcg ttcttcaagc tccttacttt cgtttttgag cgtatctatt
     4801 ttgtttttgt tgaagttgat ctcgccctgt tttaatgagc caccttcaat ttttgcttcg
     4861 cgctctgcaa tctcttttgc aatttctgca atgcgcaatt taattccttc aagatcagcc
     4921 tcaagtaatt taatactgtt ttttccatca acttcttttt cacctctgat catggcaacg
     4981 gcgtcatcgt aatgaatgcg cgggaatttt tgtgttacgg cattgcgcag gaatgctgta
     5041 ttgcgttcta atgtctccaa ttcagatgca caatgtgtaa tcacatcgtc aacaacatat
     5101 ttcagaaact gctcgatcaa atccatattc atatccagat tgtagaatgc catctccggt
     5161 tctatcatcc agaattctga taagtgtcga cgcgtttttg atttttcagc tctgaaagta
     5221 gggccgaacg tataaatctt accatgggcc atcgccaggg cttcgccata taattgtccg
     5281 ctctgactca aatatgcagg gcgaccataa aaatctgttt caaacaaggt tgaagtgcct
     5341 tcgcaggcat ttcctgtaaa aatgggggca tccatctgca ggaagtcgtt ttgctggaag
     5401 aagctatgaa tagcgaagat cacgcggttg cgcacgcgca tcatggccca ttgcttgcga
     5461 ctgcgaagcc agaggtgacg gttttctact aaaaactcca caccatgctc ttttttagcg
     5521 atgggatact cctgtgctat ctgaattatc tctacagcgc ttacctgaat ctcataacca
     5581 ccaacctggc gctcgtcttt tacaacagtg cctgtcagac aaacggaact ctccatagtt
     5641 aggcgttttg cggcttcaaa ctgctcttca ctcacttttg ctgcgtctac aacacactgc
     5701 gcaaaaccac tgccatcgcg catgataagg aataccaaac ctttgctgtc gcgcttgttg
     5761 ctcacccatc ccttgatggt tacctcacgg ccttcatatg cctgcagcga tcgtatatat
     5821 tgaaattgtt ctattgccat atctgtttac tttgagggca caaaattacg atgaattttc
     5881 cacgcataca gtctttgacc gttgtactcg ttaataaata aaagccatac atacaatggg
     5941 aaaaatgttg tcgccacaag taaaatcggc gtaaatttga cccgaaatac atcaatcagc
     6001 gtatgttgaa ccaaaggtta agtcaaaagc tcctgcagaa actctctccg cagcaaattc
     6061 agctcatgaa gctgttgcag gtgcctaccg accagttgga gcaacgcatc aaggaagaac
     6121 tggaagccaa tccggcattg gaagagggag accgcgagga acaagacgcc tccagccttg
     6181 aagaggtatt tcgcgaggag gaaaaaggtg aagaaggcac cggtgagggt gaagaaaatc
     6241 gcgaaaatga ggagctcgac ctcgacgact atattaatga agacgatgta gcggattata
     6301 aaacgcgtga tgagaattat ggcgacgatg atgaggacaa caagactaca ccatacgccg
     6361 ttaccgatac cttccatgaa aatttaagta aacaattagg catgtgcgag cttgatgaac
     6421 gtcaggagaa gatcgcgcag caaattttgg gcagcataga tgatgatggt tatttgcgtc
     6481 gcgagatttc ggcaattgtc gatgatcttg ctttttcgca aaacgtaatg acggaggaaa
     6541 atgagattat tgacctcttg gatcttatcc agggatttga tcctcccggt gtaggtgcac
     6601 gtgatttgag agaatgtctg ttgctgcaat tggaaagaaa aactcaaacc aaagccatta
     6661 aactggccat tcatatcatc gagaattatt tcgaggaatt caccaagaaa cactacgaaa
     6721 aattggcgcg acacatgcgt gttgatgaag atgatttgaa gcctgttatt accgagatca
     6781 ccagattaaa tcccaaaccg ggtggcagtg cgaaggaaga tggtggaagc atgtaccaat
     6841 acattattcc cgattttacc atcatgaaca ataacggcga acttgaatta aaactcaata
     6901 gccgcaatgc tcctgaactg cgcgtaagcc gcgattttat ggatatgatg gtggagtata
     6961 agcgcggtca gcaaaaagat aaaaaacaaa aagaagccat cctcttcatc aagcaaaaaa
     7021 ttgatgcggc gaaatggttt attgatgcta tcaagcaacg acaacataca ctgtggagca
     7081 ccatgtacga aatcatggta catcaatatg atttcttcct taccggagat gaaacgcaat
     7141 tgaatccgat gatcctgaaa gatattgccg agaaaacagg attggatatt tcaacagtaa
     7201 gccgtgttgc aaatagcaaa tatgttcaaa cagaattcgg aacctacgct ttgaaatatt
     7261 tcttcagtga atctttgtcg accgaaagtg gcgaagaagt gagtacacgc gaggttaaaa
     7321 agatattgtc tgaattgatc gaaggtgagg ataaaaagaa tccactttca gatcaggaat
     7381 tgatggaaga cctccgcaaa cgcggctata atattgcccg acgcacggta gccaagtatc
     7441 gcgaacaatt agacatttcc gttgcccgtt taagaaagga attatgataa aaaaaatatt
     7501 cgggcatttc ttttccattt tattcctgcc gctattgctg cctttgttcg gagccttgtt
     7561 tgttatttat tcagatcctt acggttttcc ggatcaaggc gccaatattt taatgcttat
     7621 cagggtggga ttgctcacgt ttttgttccc ggcaataact attttcttac ttgtcagact
     7681 gaaattcgtt cagaatgtaa cagtctatga ccgacaagag cgcacgatac catatatcgc
     7741 ctgcggattt ttctatatat gggcttttta tgttttttac aaggaagggt ttaattcaca
     7801 tatcaccttt attttgttgg gcgcaaccat tgccgtattt attgattttc tgatcaatat
     7861 tctggttgtg aaagtgagta tgcatacaac aggcgccggc ggtctagtgg catttatgct
     7921 ggtgttattt ccttatgctc aaataaatgt attgccggta ttactggctt caattgtaat
     7981 tgcaggatgt gtgggtgctg cgagattggc actgaaagca cactctgagc gtgaagttta
     8041 tcttggttat cttacaggat tcttcagttt tatggtagct tcaaatttta ttcacctgta
     8101 aacggattta tcgctgcata atctttgcgg tgcatccatc ctatcacacc atccggcatt
     8161 tctattttcg accagctact gtcggcctca attaatttta atttaaaacc ttcgtgaagt
     8221 aaagccaggt ttgtgctagt ttcggaaggt tcgcttttta ttacggcact tggcgacatt
     8281 acaattgcga atgtttggcc ggcgtcataa ttttttcttc caaaatgtcc tatagtgcat
     8341 aaaatgctaa tcgaaaatgc aatgatagct gtaaagaacc cgatgcgctg atatgcgcgg
     8401 aattgaggta acagatatgc agcccatccc attaatgcta accacatcgc aactatggct
     8461 attattcccc aggcttgtgc gcccattatt cgggaaacag cgtgccacca aataataaat
     8521 atcgatgtag tgcgtggctc catcctgtcg cgaatgcgag cattggcaag cgaaagatta
     8581 taagtagcgt catcatttcc aggatcgaga aaaagtgctc gctcataatt taatacagcc
     8641 tcgccaagtt gaccggtttt ataatagcag ttgccaatat tgtaatataa tgttgcactc
     8701 ggaccttcct gttctaataa tcggttataa atgtcgatac tggttgcata ttgacctttt
     8761 tcatacatca catttgcgta aacaaaagtg gtggtgtcaa tattatttat tccttcagta
     8821 gaagtgctgt cagggtttgc ctttaaacac aaacttgaag cgagtagtat gacgatagca
     8881 aatactttgt tcatcgcaat tcgttttcaa gtgtactgat caatgacgaa gcactattga
     8941 atgttagctc caaatcgcta tgttgaatgt ttggcgcata cagtgaaaat tcacaagtat
     9001 caagaagctg aataaattga tctattgttg aagcggaaac atgtttgttc aataagacct
     9061 gttcaatatt ttcttttgaa agggcatttt gtggaatatg caatttatcg ctcaaataac
     9121 cccacaatgc gcgaacagtt tcgtcataaa atccgcgact attattctgt tgcaaaaatt
     9181 tgccggctgc tgcaaggcgt tttttagctg tggcattcgc ttttactgct cgcatggcgc
     9241 caacatcgct cattttcgct ttttgccgcc ttgtgaaaac aaataatccc gctcctccga
     9301 tggcaggtgt gagaatcaat gccagcagaa atccgctttt catatccttg gttccggagg
     9361 aaaattttgg actatcctta tcgatgtacc gaatgtcttt ccccaacaat tctacgtctt
     9421 ccttattcgc tgcgtaatta tttgctgatg gattataacc cgggccggca gttaccttta
     9481 caggatatgc atctgtttgc aaagtctgat atttttttac tgaaggatcg aagtacgacc
     9541 atgtaaacgc cggaatggta aaatctccgg gagaacgggg aatcaataaa tattcgaata
     9601 ttttggtgcc tgaagattca cttactttcg ggtcgtatgt ttcccatcct gcaggaaggt
     9661 ctaattctgg agcattgaat aattgcaaat tacctgtccc gttaattgta atgcggtagg
     9721 tgagtggttc atcagttttt gtagaggtgc tattgatttg cgaactcatg gtgaatttgc
     9781 ctacagcccc attaaaatct gcaggtgcat ttgccggaag aggcttgatt ttaatacctg
     9841 ttgttttaga gcttaatgaa gctgtgaact ctctgctgcc actggaaaaa gggtcgctga
     9901 agaaatcatt cagcatatcc tgaaatggat cacccgaatt tttcttttgt ttttttaccc
     9961 gaacaacaac acctgcatct aatgaagtcg gatcaatcac taaattcccc gattgttgtg
    10021 gagtaagtgt tgttttttta atgataacag ttttatagtt cttaccattg atgttctctg
    10081 ccttaaattg ttcgtttctt aaatcggcat cctctacata aaaaccatcg taagcaggcg
    10141 ttttggtaat gcgatatcca tttaaccctg ctattgtacc gtcatcaggt atgcaaagtt
    10201 tcagcaccac tgtaaagctt tctccttcgt aaacatcttg atcgctgatc tctgttttaa
    10261 tgaggagatt ttcacgcaaa tattgctcaa tgtcgccgga agctgatccg gatgatccat
    10321 tattgctccc tgaattatta ttctgtgcac ccgcagttgc cggcggcgca tctgttactt
    10381 cgattactat gggttgcgat gaataggttt tatccttcac tttaatataa gcagcgccga
    10441 tttgaaattt tccggttttc ttgggctgca gaatataaga gaaactcatc gactgttgca
    10501 ccgtattatt aaacatctga acactggtcg attgatttgg ccctccaagt acagtaaaat
    10561 cggtgaaaga aggcgggcga aaattttcag gattgctgcc attgttcagt gtgataataa
    10621 actgaaaacg ctcattcaca ctcaccttgt ttttatttac cgtggcagtg aatgacacct
    10681 gtgcatgcaa agattcaggc aatatacatg ccatagtgac taaacacatg aatatcagca
    10741 cattacaacg tctcattacc agtctttttc agggtcccct cccgcggggc gggctttttg
    10801 tttgttcact ttatcttgca cagctttatc gtcttcattc agcgatttta atatcctgtc
    10861 gagatctttt ttgtcaggcg tattaggctc aggttgttta tcctgattgt tgttttgcgg
    10921 attgttatcc tgttgctgct gcttttgctt ctgctgttgt tgctgttgtt gtttttgttg
    10981 atcttgtttc tgctgttgtt gctgttgttg ttttttcagc atttgttgtg catacgacag
    11041 gttgtatttt gtatcggcat cttcaggctt atcgcgcaac gattttttat aggcatcgat
    11101 acttcccgag taatcttttt tttcaagtaa ggtatttcca aggttatgat atgcctgtga
    11161 acgaatttca gggtctttag ttccttcggc ggctttactg aactgttcgg ctgctgcatc
    11221 atattgttgc tgcatataca agctgttgcc aagattgaag tttccttcta ccgattccgg
    11281 tgaggctttt acggcttcct gataattttc acgggcgcca tcgtaatctt gcttatcgta
    11341 tttggcattg ccttccttga taagttcatt ggcattttgt gcagtaagcg tccgcacggc
    11401 aaaaatgaat gctattgcca acaggtgttt gatattattt tttttcatca tcaaacaaat
    11461 caattttttc tacccacttc ggttttttat cggtcatgag gaattccaaa attaacagga
    11521 acaagccaat tcccagaaat aattggaagt ggttgacgta ttcagtaaat tcaaaatcgc
    11581 catcaacagt cttatcgagc tcatcaatgg ttttatatac ttcttgtata actctttttc
    11641 cttcggcaag gttcatgtaa acgccatctg catctgtagc cagatcgttt aacatttgtt
    11701 cattgagttt ggtaagtaca atattgccgg agcgatcttt cttaaatcct tcaatattat
    11761 tattcttacg tagcggaatc ggaccgccag tggatgtgcc tacacccagc gtaatgattt
    11821 tgattccttc cgaagcggct ttttttgcca tctcttctgc attgtcgtca tggctctcgc
    11881 cgtcggtaat gatgatgatg gcgcgaccct ttttatcatc tttctcccca ccattttcga
    11941 acgagagcat ggcggtttca attgcacttc cgatagcagt gccttgcgta ggaacacttg
    12001 ttgtgttgat ggtgttcaaa tacatcatgg cagcacgtgc atctctggtt aaaggcattt
    12061 gcacataact gttacctgca aacactacca gaccaacttt atttgcggca agtttttcaa
    12121 tgagttccga agcggtgaat tttgcctgtg taaggcggtt tggtttcaca tcctcagcca
    12181 gcatgctgtt cgaaacatca aaacaaataa ccacctcggc actttcacca cttacctttt
    12241 gttctttaga tccctgacga agattggcaa tgcctaaaat gagaaaagca atacccaata
    12301 attgaagact atattttatc catctccttt tatcggaatc gcggaacatt aggtcgcgaa
    12361 tcaattcact atcaccgatc tttttgatcc ttgccgtacg ccacctcaaa taccaccaat
    12421 acatcagcag caatgcagga agcaatatga gtagatataa tatgtcggga cgttcgaatg
    12481 acattatgtt agtgtgcgga atattgtttt tgaaagtatc acctctagta aaatacaaat
    12541 taacccgcct actgcaaacc aataaaaatg ttcatcgtaa cggcgcgaga tatccattga
    12601 aaatttggtt ttttctattt tatcgatctg ttgatagata tccgttaatt tttcttttga
    12661 atcagcttcg aaataggagc cacctgtaat cgacgccaca ttttttagca ttggcgcatc
    12721 aaaggaaatt cgcggatcaa gatcaaaaat atgtctaccg tttttatcga acaccggtat
    12781 cggactgtta gtatttgtgc cgatgccaat ggtatacacg cgcatgttca attcctttgc
    12841 accattggca gcatcaagtg gcgaaaattt acctcctaca cgcacaccat cagtaagtaa
    12901 gatgatcact ttctgagaaa tattggttga atccgccagg cgatttactg ccatgaataa
    12961 tccgtcgcca attgccgtgc cgtctttcaa ataataattg tcggcattgt caacttgctc
    13021 cttcaataaa ttgtgatcga gtgttgaagg acattgtgta aacgcctcgc ctgcaaatac
    13081 cactaaaccg attctgtcgt ttggcctgct gtcgataaat tcttttgctg ctgtttttgc
    13141 agcctccatt ctgtttggct tgaaatcaat ggcatacata cttggcgata cgtccaatgc
    13201 cagcataata tcaattccgt ttgaatacat tctgctgtta ctgtaacttg tttgtggacg
    13261 agccatggca atcacgataa gcgcaaaggc aagcaaacgc aatattaaaa gcacagacat
    13321 aaattttgct tttccttgcg gagcttttgg tatgccgctt gtggtagaca tacgcatggc
    13381 aacaaatttc ctgcgctcta tttttgacct gaaatataca tataccggaa tacctgccag
    13441 taacagaaga aaccaagggt cggcaaattg tatgtcgctc aaaaagctca ttcctgtttc
    13501 ggtgtttcag taattatttc gcgtgtttgt tcaatgaagt tgagcacatg atcgaatgct
    13561 cgtgtatttt catcagcaag cggctgtgac ttcgcaaatt ttgccatatc tgcaagatta
    13621 agcagatacc tgatattaga ggcatgtgcc cttgtgtcgg gatgtctttc caattgctca
    13681 actatttcat cactggtgct ttcatgtgca tgcatgtgat agcgctcttc taaataatcg
    13741 cgcaagatat cagtcagctc tgaataatag ggtttcaact gacctttctg ccatagttgt
    13801 ttgctttgca attgttgaag gcgttccgtc atacgctcat gcaatgattt tagtggaacg
    13861 ggtacagcaa tgggtccgat atttttcctg cgcaggaaaa aataccaaat caatccacct
    13921 attataatta atcctgtaat tattcctacc caaagtatcc atttatgatt gcgtagcgta
    13981 acatctaaca ccgattttat tggtttgatg gcaagtgtag tgtcaacggc aattgcttca
    14041 atatgcaagg gaatagaatc agtgagtatt tgatgttgag cggtatcact tgtagactgg
    14101 taattaattg ctacctgcgg aaaatataca tcgcctcctt cgtagatact gtaaatgata
    14161 tgctttgtca ggatgacacg gtcgtcgctg cgctgtgtat ctatcggcat gctgctgatg
    14221 atgtcgtagg tgctgattgt ggtttcatca ggaaaataat atttaaagga tgccggcccg
    14281 cttatggtaa tatctacaga aatataatcg ccaatgctat atgaaggttt gtcggtatta
    14341 gcatgagcag atattatctg accctgaagt tgattacagg taattattat caataatatg
    14401 acaattaatt tcttcatcat ttatcgcatg ctgattcttt tcttaaaaaa ggcgtgtagt
    14461 gcattgatat acgataattc actgtgtata gaaatggtat cagccccact gcgcaaaaag
    14521 gtgtttttga aatactgcag attggtgttg aagtatttct tataggaagc gcgaacagtt
    14581 ttatttgaac tgtctaccca ttgaatattg cgtgattccg ggtcaaacat gcgaatgatg
    14641 ccggcgtccg gaagatcttc ttcgcgcttg tcgtgaatat gtacacctat tacatcatgt
    14701 tttctcgccg ccaccatcaa tggcttctca taatgcatat cgaaaaagtc gctgaacata
    14761 aatgcgatac tctttttctt caccacatta ttgaaatatt ccaatgccag tgcaatatcc
    14821 gttcctgagc cttcagcttt tacttctaac aggtcgctga tgatacgaag tatatgtgac
    14881 tttccttttt ttggtgggat gaatttttct attctgtctg aaaataatat caccccaacc
    14941 ttatcattgt tattgatggc agaaaaactg agtaccgcag caatctcggt gatcagcgct
    15001 ttttttgttt gcttctgtgt gccgaataaa gaagatgcac tgatatcaat cagcaacatc
    15061 accgttaatt cgcgttcctc ttcaaatatt ttaatgtaag gatggttcat gcgagcggta
    15121 acattccatt caatattgcg aatgtcgtcg ccataagcat attcgcgcac ttcactgaaa
    15181 ctcatacctc tacccttgaa ggcagaatga tattcgccgc taaagatatt ctgcgagatc
    15241 ccttttgttt tgatctcgat gagtcttacc ttttttaata aagttgcttt atccatccgc
    15301 tttagatcaa cgtttctttg tggtgatcat taaggcacct ccactacatt gagcaattca
    15361 gatatgattt gttctgccgt tatattttca gcctcggctt catatgtcaa tccgatgcgg
    15421 tgacgcatta catcgcgaca aacagcacgc acatcttcag gaattacata tccgcgtcgc
    15481 ttgataaatg cataagcctt gcttgcgaga gccagattga ttgacgcgcg tggtgaccca
    15541 ccatatgcaa tcaaatgttt cagcttacca agtttatatg cttccggatc gcgcgttgca
    15601 aatacaatat cgagaatata gttttctatt ttctcatcca tatatacttc gcgcaccagg
    15661 tctcttgctt tcagaatgtc ttcaggtttt acaacttgtt taatttgttg tatggcaccg
    15721 ctcacgttct gacgaatgat cattttttct tcctcttttt caggatagtg tatcactact
    15781 ttcagcataa aacggtctac ctgtgcttca ggaagcggat aggttccttc ctgttctatc
    15841 ggattctgtg tagccagtac caaaaatggt tcttccagtt tgtacgtatg ctcgccaata
    15901 gtaacctgac gttcctgcat cgcctcgagt aatgcactct gcacttttgc cggagcacgg
    15961 ttgatctcgt cggccaaaat aaaattagcg aaaataggac ccttctttac actgaagttg
    16021 ttgtccttct gattataaat catcgtaccg atcaaatcgg caggtaacag gtcgggtgta
    16081 aattgtatgc gcgaaaactt tgcattcacc gccgatgaca atgatttaat ggctaaagtt
    16141 tttgcaagtc ccggaactcc ttccagtaaa atatgtcctt gagataataa tccgagtaac
    16201 aagcggtcaa tcatatactt ctgaccaaca attgctttgg aggtttccat cctcagcagg
    16261 tcaataaaag cactttcctg atgtattcgc tcattcaact cccggatatc ggttgatatc
    16321 actgcatcca taataagtag gtttggtctc gtagaagaaa acgggtgaca aaattatatt
    16381 atataaacaa gttgaaacgt gtgtacattt gacctgcaaa gttaaaaaag gttaaggtcc
    16441 gtataactct tgttaagtgg gttacaaacc ttgattttca ataatttcca ataaactgaa
    16501 cactacatga cagatttcat ttccattgag gcaacattag gtgccgaaaa ctataaaaca
    16561 agcatgcaca acggcagaag cacggtgttt tgcgatgaac ccgctgataa aggcggaaaa
    16621 gacagtgctc ctgcaccgcg ggaattgttg tgtatgagcc tggccgcctg caccgctatc
    16681 accttgcgca tgtatgctga aaggaaaaac tgggcattgg gtaatatcga tgtgcaagtc
    16741 acgctttcag aaggagaagc aggcaaacag gtcttcaaca gaaccatcaa gcctgaacat
    16801 aagcaagatc aggctattta tgatcgcctg ctgatggttg cagataaatg tccggttcac
    16861 aaattgcttg ccggcacaaa tgaaattata acaaccatag cctgatgcaa gacattacaa
    16921 gtagaagcga tgttgccaag attgttgtgg aattttacaa tgatgtgcgc cgcgatgaat
    16981 tgctggcata tatttttaat gatgtggcgc aggttaattg ggaaacacat acaccgctta
    17041 tcatagattt ctgggaatat accgttctgg gaaccggatc gtatacacga aatgctatga
    17101 caccgcatgt tgcgctgcat gcaaaatcgc cacttactca agcgcatttt gatcgctggc
    17161 tgcaattatt tcacgctaca gttgataaat attttgcagg agaaaatgcc gacctgatga
    17221 aaaccagggc tgatggaatc gcaggattga tgcatcacaa attaaatggt tgattgcatt
    17281 tcaacccgga tactctacga tattattttc ggtttcgtat agtgtcacct ttagctcaag
    17341 atctgctgcc agtgcacttc tcagcttttc gtagataatg cgtgcaatat tttcggcagt
    17401 aggattcaga ttgcgaaaat cgggcacgtc aaggtttagg ttctgatgat ccagaggctc
    17461 tatcacgtat gtgttgatca gaatattgac atcttttaag tccattacat atcctgtaac
    17521 cggatcgact tcacctgtaa tttttacaaa taacgtgtag ttgtgcccat ggtaattcgg
    17581 gttgttacat gcaccaaaaa ccttgctgtt ggtagcatca tcccatgcag gattgtgcaa
    17641 tctgtgtgct gcattaaaag ttgctcttct tattactgat gtcttcatat gcctactaac
    17701 atccaatatc acacaaaggt tgctaaaatc ctttacattt gtgcatattg attatgcaag
    17761 aaaaatcttc tttacatgca tttcaatttc cttccatcga tggcggtatg atcgattttg
    17821 cggtgtttaa aggaaagaaa gtgcttgtgg taaatacggc gagcgattgt atgtataccg
    17881 atcagtacaa acaattgcag gaattatcgg taacctaccc aaacgaattg gtggtggtag
    17941 gctttccgtc gaatgatttt ggtgagcagg aacccggaac aaaccgtgag ataaaaaatt
    18001 tctgttcata tcgctatggc gtcacctttc ccctggcttc gaaaagtccg gtgattggga
    18061 tggatgcaaa tccggtgttc aagtggctta caactgttcc gtttaagacc gacatgaata
    18121 aaacggtgac atggaatttc caaaagtttc tttgcgatga aaacggggtg cttatagagg
    18181 tatatcctcc ggcagctgat cctataaacg atgggctttt ggaacatatt aacccgaatt
    18241 gataacataa aatcttcgga tgttcttcag ccaggtcata ggacaagatc gcatcaaaca
    18301 gcagctgatt cgcgctgttg aagaaggacg ggtgagccat gcccagttgt ttttgggccc
    18361 tgaaggctgc ggtaatctgg ctatggcact ggcttttgct cagtacatca attgcgaaaa
    18421 tcgccaaact ggcactgatt caaatggcaa ggtgacaggc gattcctgcg gcaaatgccc
    18481 ctcctgtatc aaggcgcaaa aagtgatcca tcctgatatt cactacactt ttcccttcgt
    18541 caaaatagaa gacaaagaac tatgtgccga ttggatgaag gaatggcggg aatttgtgtt
    18601 gcaaactcca tatggcactt ataacgattg gatagaaaat attactgaag gcaataaaca
    18661 aggcaatatc accgccaaag agtgcctgga gattctccgc aagctttcac tcaagaacta
    18721 tgaaggcatt tataaagtgc agattctctg gatgcctgag tttctcgaca aggaaggcaa
    18781 ccgtctgtta aaaatgctgg aagaaccgcc tgataatacg gttttcttac ttgttgccaa
    18841 tgacagcgaa cagatattga ataccattat gtctagggtg caaatcgtca aatttgcgcg
    18901 acttgctgat gaagatgtgg cacaagtttt ggagcgggat caccaacagc ctcataatca
    18961 agcagttaag atagctcaga tggcaggtgg taaccttcag gaggctatac gtttgttaga
    19021 tattcacgag gatgctaata ccgcaatgat ccgggagtgg atggaagctt gctatcgcca
    19081 gcaggtaaag cagttattcg cgtggaacga caagttttta aagctgggaa gggagcagca
    19141 gaagatgttt tttcaatact gcctgcactt tttccgcgag gtaatgctta ttcaggccgg
    19201 cgcagggcaa ctcgccaaac tcacggacgc ggagttgaaa actgcccagg gattaggaca
    19261 ggtgcttggg gttgagaagc tgatgggtat tattgaatta tttgacaaaa gtatttactt
    19321 tgtggaacgg aacgttaacg gcaagatcat gatgacgtac gtttcggtga cactgttaaa
    19381 gcagtttcaa aagaactatc agccaacgcg gcccttctat gatcggtggg ccctttaaaa
    19441 tatattacta tgagttgtgc aggatgtgct gtgggcacgg ataaaagctc cccggccggt
    19501 tgtaaaagca acgggcactg cgctacggga ggatgcaata aactgaatac tttcgattgg
    19561 ctgcgcgcca tgccggtgcc ggtatcgcag cgtttcaata tacatgaggt aagttttaaa
    19621 aatggtgtcc gcaaaggatt ttatgccaat accaagggca tagatgtgat gacgggcgat
    19681 atggtatgcg tagaaacaga aagtggttac gatgttggca ggatttctct cagcggcgaa
    19741 ttggtgcgac tacaaatgaa aaaacgtcgt gtttcgccta acagcacttt cagccccatt
    19801 ctaagaaagg ctaccgatgc tgatatggag aaaatgatgg agggaagagc gcttgaaggg
    19861 gcaaccatgc tcagagcacg cacctatgcc cgtgaattga aactcgatat gaaaattggg
    19921 gaagttgagt tccagggcga taagcgcaag gccacatttt attatatcgc agatgatcgc
    19981 gtcgattttc gtgagctcat caaaatatat gcaagagagt ttcgcgttaa aatagaaatg
    20041 cgacaaatcg gggcaagaca ggaagccgga aaaattggcg gacttggaag ctgtggacgt
    20101 gagttgtgtt gctctacatg gctaacagat tttaaatcgg tgagcacgca tgcagcgcga
    20161 taccagaatt taagcatcaa ccaatcgaag ttatccggac aatgcggtcg cctgaaatgt
    20221 tgtctcaact ttgagcttga tacctatatg gatgcattgc aggattttcc aaagcatgca
    20281 gatattttgc gcacagaatt tggcgatgcg cgattagtaa agacagatat tttcaaaaag
    20341 ctgatggttt atgaatataa tgccggaggc aagcaatacc ctgtaacact tgaaaaagta
    20401 cgtgaggtgt tatcgatgaa cagtaaaggc caaaaaccgg agcatctcga aggggtaaaa
    20461 tatgagcaag agcctgcagt taaggaagtt gaatatgcta cagttgtaga acaagttagt
    20521 ttgcgcacga ttgaaaaagc ggaacaaaaa cgccgtcaga aaaatcgtca gaaaggtccg
    20581 cagcaacaaa atcagcgacg tgacaatcca cctcagggtc gaagagataa caatcaaaat
    20641 ccccgtgatc agcgtcagca ggagcagaaa ggaaataatc aaccgcgccg cgacgggaat
    20701 cagcaaaaag gtccgggcaa ccagcagcaa aatcaacaac gccatcgcgg aaataatcgc
    20761 cccccaagag gaaataatcg cggcccggga aatgataaca acaataaatc ctgaaggtga
    20821 ataatcgcgc ctatagcata ctattttctg tattggtaac atgctttgta gccgcaggtt
    20881 gcgaacagtc gcgcgtgttt gataaaaata caccgatcga taaaagtggt tgggcttatg
    20941 gcacggcact tagttatgag gtgcagatca atgacactac gcaaagctat aatctctata
    21001 ttaacgtaag gcataccgat gcatatccgt ataataatat atggattaac atgactacta
    21061 ctttccccga tggaacaaaa caaactacca aggtagacgt gcctctgtcg gaacctgaag
    21121 gtgagtggat gggaaattgt gtcgacggca tttgttttaa taccacattg attaaatcgg
    21181 acttcttttt accgcaaaaa gggaaatata ccttcacatt ggagcaagac atgcgcatga
    21241 atccggtgcc tgatattttg gatgtaggta tccgcgtgga gaagttcatg gtgacaagat
    21301 gatcacattc gacggtcgaa ttattataat cacttagtaa attccttatt ttcgtatttc
    21361 aaatactccc aacatgcaca ggcagacact cattgcaatt ttgctttcaa tttcatttgg
    21421 tattagggct caggctccca cccaaccata tatcatcatg attactatgg atgatatgaa
    21481 tgcatatctg ggtgcttacg gaacgcatcc gcaggtggaa acgccaaatc tggatgcatt
    21541 gctgtcgatg ggaacaattt ttacaaatgc gcattgtgct tcacctaaat gcgccccttc
    21601 cagaacttct gttatcacag gaaaggatgt tgattatacg caagtatatg acaacacctc
    21661 ctatgcatgt cgcgatttcc gaaaaaattt taaggtggct tacggcaatg ctgaagtatt
    21721 tacattaccg gagtatctga aagataacgg gaactattat acctacacca tgggtaaaac
    21781 cctgcattgt tatgaagctt attccgatta cgataccgtg actttagatc catgcgaaaa
    21841 acaattttca tggaataatg catttgtgta tgttgaagat gaaatcatag acccaatcgg
    21901 agatgcagaa aatgaaggca tctttttatt aaaatgggca cgactcaatg ataccatgat
    21961 tgattatatg gaagatgatg ttacggttga tcatgctatt tctttcatta atgatttttc
    22021 aacttttggt acatcaatta catgcgacag gccatttttc ctcgccttgg gaattaaaaa
    22081 accgcacgca cctttttatg tgccggagag ttttttcaat gaaaattatt taaatagtgt
    22141 atatgacgat ccatatgaca ttccatataa tgttccttat aacgctaatc catacaatgg
    22201 catcgtaatg cctcctcaac ctgaacccag atgggctgat tatgattcat tgggatatat
    22261 ggggcagtta tttgcttctg ataatgaaga gggacaattt gaggccttcg gcgaaagttt
    22321 gccttcattg cctgaaattc aagcaggtat ttctgattcg gaacgcattg aaatattaaa
    22381 tgaatcaatg cgcgcaaatg ctttgatggc ttatctggca ggagtgaaat tccttgatta
    22441 tgaaattggt agatttttag atgcattgga atcacatcct gaaatatata ataatactgt
    22501 tatcatcatc accgccgatc atggttttag ccacggtcag aaacgccatt ggggaaaatt
    22561 ctctttatgg gaacccgata tgcgtgtgcc tttggtgtat attgatttga gaaatccggt
    22621 tgcacaaact tgtaatgcat ctgtgagcaa tatggatatc tttccatcgg tattagatat
    22681 gctggatatg gctgaaccaa catttttatc cggcgaccgc tatttagatg gatatagttt
    22741 catgccattg atcaacaatc cgaatcaacc ttgggagcgt ccggtacttg ccggtgtgcg
    22801 cacaaaaaat agcggaggca ctttgaacga tggctcatgc tttacacagt attctttgcg
    22861 taataatcga tttcattata ttaaatatgc cactaataac gcgcctccgc tgcttacctg
    22921 cgatttcgct gctagtcaaa cagaggaaga actttatgat gtaggtgtta atcgtgaaac
    22981 ggatcccaat gaatggaata atctggttga tgatccggaa tacgctccgg tattgaatta
    23041 catgcgtcaa tttattaaag acagcacaca ttacaaggat cgcatgtttg cagttaacat
    23101 aaacaataca aatacactgc cttgctttgt gaaaaatacc ggtaagatta aattacaggc
    23161 agcactttat gatttgaatg gagcattgtt gaccggcgca ggctatactt tcacctggac
    23221 taatagttta actgcagcta ccgttgcggg aaaaaattac acttttaata tggcaacagt
    23281 gccgcccgcg gtttatgcgg cacatgacca tatcatgttc tatctgaaag taactaatac
    23341 tactacaggc gaattggttg catttaatac cagaacattt tatattaata atgcaaatac
    23401 ccctaatgcg gtttattcat tggtaacaga taatatcgca ctgactgctt ctattacagg
    23461 ttacacctta accggtagtt acaataacac ctattggaat ttcggcgatg gaacgcaatc
    23521 ggaggatttc ctgcctgaca ctcattatta tgcagcaccc ggattttata ctgtcagaaa
    23581 ttatattcag tacggaaatg gatgtttgaa aaatgttgcg cgcaatgcaa atctcactcg
    23641 cgaaggtgta ccccaattgc aatttgcgct gtatccaaat ccggcaaaac aatcagttac
    23701 cattgcatta agtgaggcaa tatcagatgc cgatatcacc gtttataata tgcttggtca
    23761 accagtttat atgcaatcaa taaaagaagc agataggtca tttactatcg atattaccga
    23821 catagcaaac ggcacctata tggttgagat tagtacacca caattttatg gtaccaagac
    23881 tttggaggtg ataaggtaat ttttaccagt ttacttcgag taattgtggg cagtgatcac
    23941 tgtgctttgc ttcgggtatg atgcgagcac ctgcaagttg ttttttaagt gattccgaaa
    24001 cagcaatgta atcgatgcgc cagcctttat tatttgctcg tgcatttgcc cgaaaactcc
    24061 accaggtata ttgatgtggc tctgaattga attgtcgaaa actgtcgaca aatccatttt
    24121 catagaattt atccatccag gcacgttcct ctggcagaaa tcctgaagat tctttgttgc
    24181 ctttagggtc atgaatgtcg attggcttgt ttgcaatatt gtaatcgcca caaacgatca
    24241 gctttcttcc ctttttgttt aaggcgataa ggtagtcgta gatataatct agaaactgat
    24301 attttacttg ctgacgaaca tcgcctgtgg tgccggatgg gaagtaaata gagaaaacgc
    24361 tcttatcacc aaaatcagct cttatcacgc gaccttctgc atcatatgcg ctgttgccgc
    24421 atccaatttc tacgtgatca ggttttacct tgctgagaat ggcaacccca ctatacccct
    24481 tcttttgggc cggatgccag tagtgatgat aacccagatt gtcgaaaatg ctcaaatcca
    24541 actgtccggg ctcggctttc agttcctgga ggcaaatgat gtcaaatccc tccttctgca
    24601 cccattcggc ccatcccttt gtcagcgccg ctctgatacc attcacgttg taggtaatga
    24661 ttttcatgat catagttatc gtccaaaggt aatattgcca attgattgat aataactgca
    24721 agcgctcgag tttgcagctt ttcgtaactt taacttccgt ttgctttgca aaataacttg
    24781 ttaaaatatt atttcaacgc ccattttatg ggaaacttat cggaagctcc cttaaaagtt
    24841 caaaataccc atgcataaaa cgttaaaatc ttttttcaca tgttctctct ttttgctctt
    24901 gggttggcga acgggatctg cacaactcga tcagccaaat tttattttta tagttgttga
    24961 tgatttgaat gattatgtgc aaggtattac cgatcagcca caggtgctca cgccaaatat
    25021 tcaggagctt gcgaatagag gcatgctttt ttcaaatgca tatgctaatt cgccgggatg
    25081 cgctccttcg cgaacaagtt ttttaagcgg gaaggatatc caatatacag gtgtatataa
    25141 taatgaggat tatgcctcta aattcagaga gaatttttct gttgccaatg gaaatgaaat
    25201 agtttattcg ctgcctcaga tattaaaaga tagtgcagga tattttactt atggaatcaa
    25261 taaaattttc cataatccga ataataacga ttttgataaa actatagcaa cgccggcatg
    25321 tgataagacc ttgtcgtgga atcgcatgga ttttattgaa gattccgatt ccttgctgga
    25381 agcattatct gtttatggct ttggcaatac atttgattgg ggtatgatac cagatagcct
    25441 tgaaccttta ctcgaagact atatcggaac aaatcttgct attaacttca ttgattccgt
    25501 agcaaccgga actgcagaaa cctgcggcgc tccttttttt atggctttgg gatattataa
    25561 gccacacatc gagcggttta tcccggaaaa atattttcct gattattatt taaatgatgt
    25621 gtatgccgag ccctttgttt taccttataa taatcctgta gatgcctatc catacaacgg
    25681 aattgtaatg ccggttcaac ccgatttgtt atacggcgat tactataatt taccggaagg
    25741 tggaattgca cgtgcaatgg ctgataatgg cggagtctat gatcagatag aaacctatgt
    25801 tacaggtctg gatcctctcc ctgttatcga tgacctgctg acagatgagg atagaatgaa
    25861 tatcctgcgc actgcaacag ctgccaatta tgaaataaat tatatcgctg caattcaata
    25921 tatcgatgca caacttggca gattgatgga tgcattggag gcttatcctg caatatatga
    25981 taatactatc gtggtattat taagcgataa tggctattct ctcggcgaaa aacgccattg
    26041 gacaaaatgg acattatggg aaccggatct tcgtgtgcca atgatcattg ccgaccctag
    26101 tcgcgaaggt ggcgaagtgg ttgaacagac ggtttcatta ttagacttat ttcctacagt
    26161 atgcgatttt gcgcatgtga actaccctac ttttcctgat ggttctaaat atctcgatgg
    26221 gcatagtatt gctatgttgc tcgatgaacc aaatcttaaa tatgagtatc caagtttatc
    26281 gtcctataaa aagaacggcg gtatcggttc atgctatccg catcattctg taagaaatga
    26341 acgctggcat tatatccgat atcgtgaaaa taatgatggc acttttgcca ccggattctg
    26401 tgattcgata aatcacagct atcaacagga attatatgaa ataggtatcg acagagaaac
    26461 agatccctat gaatggaata atctggcaga agatcctgat tatgcaccgg taatcaattt
    26521 tctggagcag tggatgccgg atagtgttta ttatttaaaa aaggctttca gagtaaccat
    26581 gcagcatgat gctatcgact gtcttctcga ccctgaaggc tcagtgcatc tttctgcttc
    26641 tgtgtttgat acggcaggaa ttttatttga tcttcccgat actatgcagt tgagatggta
    26701 taccaataaa tcgcctgaat atacttatgg tcaggtagtg gatattgatt taactacaat
    26761 tccggatggt gcttttgata tcgatgccga gctcatcatt tatgttgaac tgataggtgc
    26821 cgatagtaat atcattcaag gtttcgacct gcaatatttc tatgccgatc ctatacatgc
    26881 tcctgaggca acttatgatg ttgcctatat tgatgctttg acaattgccg taacaggcta
    26941 tacccttaca ggaacatatt atacttcttg gtgggatttt ggcgatggca ctacttcgcc
    27001 ggcaacggac cctatcaccc acgtttaccc cgatgaaggg ccgtataccc tcaccaattt
    27061 cgtgagttat ggcaatgaca cctgtgttgc tgcattcagc aaggagatat ccttcacaac
    27121 agatacttca gaccctgaaa ttcaactatt ctgctatcca aatccggttg atgatttgct
    27181 aaggatctac cttccggtgc aatctgcata cacggctgta gacgtatttg acctggccgg
    27241 gcgacctgtg ctttcgcaac ggtttattac agctaatacc ttgttattca tatcgttgga
    27301 ttgcagtaag ctcaacaatg ggatgtacat tgtatgcctg aataccgatc agggaaggta
    27361 ctccgcccct gtagttgtat tgcatccatg acagaatagt tacatagatg tattgttaat
    27421 gttgtgtgga atactaaagt ttgctacttt tgggttaagt tagccgaaat ccaacgtatg
    27481 aaaagaatcc tcgcaaccct cctcctcgcc ggttccgtac tggttgcctc agcgcaggat
    27541 atccattttt cgcagtattc tcagtcacct ttgaccttga accctgcgtt aaccggactt
    27601 actccctgta cttatagagg tgtgctcaat tatcgtaacc agtgggcttc tgcattggga
    27661 cctgcaagtt atcagacctt tgccggatct tttgatgccg gtttgtggcg cgataagttc
    27721 gatggaaata ttgttggtgt tggtgcaatg atgtataacg atgtgtcagg tgatggttca
    27781 ttaactaaca tgaccgttat gggttcattt gcctatcatc aaatgattgg agatgaaaac
    27841 aattatctgt ctatcggtgc gcaacttgga ttagtgcaaa aacgtgtaga ctactcaaaa
    27901 ctcatattcg aatcgcaaat tggagttaat ggtgttgatc cgaatcttcc aagcggcgaa
    27961 tatggtgatg ataatttctc atatcttgat gtaaatgcgg gtattaactg gagaggtcgt
    28021 ttttcagata aattcgctgt tcaaatcgga ggtgcctatt tccacttaag tgaacctatt
    28081 gaaacattct acaactacaa tgtaaataag ctgaatgcgc attacgtggg atatggatct
    28141 ttcaaaatcg gtatgggaaa aagtacagta ttgattcctt cattccttta tatgcaacag
    28201 actgaagggt caaacgttca aataaactca ggtgttgatg tcggcttcca actcgaaaaa
    28261 ggtgtttcta tggcttatgc cggattgcac tatcgttcag tggataatat cgatgttgcg
    28321 gatgcggcca ttattaacct cggattagat tataataaca tcaatttcgg tttaagctac
    28381 gatgttaata tttccggatt aaatgccgta accaattatc gcggtggtat cgagttgtcg
    28441 ttcgtgtatt ggggttgcct cagcgtaaca ccaaaatcga aacctatcga ttgtccacga
    28501 ttctgatcaa accataaata ctacttcatt ttccggtaca cgaaatcttt cgtggtaaat
    28561 accttgtgtt gtaccgggtc cgcaactgat gatcatattg aactcagtgc ttcttggcag
    28621 tttaaggtat ttgcgcaagc gctttgaatc aaaaccttcc atcggacatg tggcatatcc
    28681 ttctgctaac atgctaagca taaatgtttg tgcagcaagt gcagctgatt tgtgacatac
    28741 tactcttaaa tcctgcttgc ttacctgcca aactaccgga cgccaaatgg aggcaattga
    28801 tactattaat ttgcgaactg caccccataa accgagccaa tcctggaaat aatacatcgg
    28861 catcaaccgc gaaaagtact tcagctttct tgctgaacgc ttatccaact cttcgattgg
    28921 tttattacca tatcgtgcgt gatgaaatgc ataattcgct tgagcacgcg ctctgtattt
    28981 atttttcggc acagcaataa tcaataattc tcgagcagtt tttgccgcat tctgattcat
    29041 gcaaaatact gcaatgtctt ttattttttg cggatcggaa acgcgataaa attcccacat
    29101 ctgcatgttg ctgctattag gcgcaagcat tgctctgcgt atacttcgtg caaccgcctc
    29161 actatcaaag taagcctcgg catcataaat gcgcatactg cgacgagatt gcactatatt
    29221 atcaaaaact gttgcagatg catccattgt aattcgttaa ttgtagaaga aaattattat
    29281 ttaatgtcga gatattactt aatgctatag cgcttgaaat ctttggtcgg ggcattaatg
    29341 tataaatgat ttccatcgga actgaattga acattgtgaa tccagacatg catgtcatta
    29401 aaagataaaa cagattccca tgtttcagtg tcccagattt taatagtgcc atcgccacca
    29461 cctgaagcga ggcgctttcc atcaaccgaa aacgaaacgt cgtaaacaat gcctgtatgc
    29521 gcgttcagct gtttcaaaag tttacctgaa tcggcagcta aaatatatat cgttccattg
    29581 tcatctgtgg caataatatt ttgaccattt ggtgaaaaaa gtactttttg taaatgattt
    29641 ttagaaatcg ttgtgttgaa cacaaattct tttgtttcca tattccatac accaattatt
    29701 cccaaggatg aaacccctgc aatcattttt ccattgtagc tgaaacttag atccatcacc
    29761 tgtccggcga aatgaatttg ctgaaaaatg ccgccgctga cagcattcca aatagtgata
    29821 ttactatcgg cactcgctgc agctaataaa gttccatccg gattataatc aaggtcgtaa
    29881 acagctcctt ctacgccaaa ataatcatca tatcgtttac ccgttgctgt ttcccaggcg
    29941 cgaacatgtc cttcgttgtc gcatgctgca attaaatgat tgtcgtgatc tattgtgata
    30001 tcattcatta tttccggcag cgatgggaat tgcttttcta tataacctgt tgcattattc
    30061 cattgcgtaa tgagtccgaa ttctccctgt gtaaataaat atttactgtc ggcagaaact
    30121 tcaaaggcat aggtcaatcc gctgaaaccc attgggttaa tataagtgcc tgctttcgca
    30181 agatcccaaa accttaatgt gccatcggca gacagtgaaa ccaatacatc accgttttgt
    30241 gtagtgagca gtttttttac ttcgccgata tggccaaata attcttgctt gacagtggcg
    30301 ctttctgtac tccacaacgc aatgctacca tccgaagcac cggttaccat gccgaatcca
    30361 tttggtgtgt aacctaccgt ggtaatattt tgttgagcac tatataattt ttgctgtgtt
    30421 gttcgggtga acatattcca aacttgcgca aatccgtttc cattagccgc cattaaaaag
    30481 gagccatctt cactaaaggt gatatcttta atttgttgaa tggaattatt atcatcgaaa
    30541 atgtaattct gcacaacagt aaaactatca gtgcgaatta cactgattac gccgttttct
    30601 tctccataag caagcattga gccatcattg ctaaatgcag acgcaagagg tttttctttt
    30661 ctttcgccac ttttccagat atcacttccg gtttgtgcct gtaacactat ggtgtttcct
    30721 gtatagccat atccattgaa tagggtagtg ctgacacaaa tcaatttacc atcccgacta
    30781 acagataatt tatttattcc tgccggaatt gatttttccc agaccttcga cttgtctgtt
    30841 atttttgttg ctatcacctt tgcatcctgg ccggaggaat aaatgatttg cgatgaagga
    30901 tcaaaagcta tgctatatac cttgccttga tgtgtagtaa ttgtagatgt ttcagattta
    30961 ctaaggacat catatatatg aatattatga tcagctccgg cgatagcaat agacttgcca
    31021 tcgggtgaaa ttgccatatc attagcaatg tagccaagtt caatttttcc tatactctgg
    31081 tcgagcgtgt agtaaagata attccattcc caggctctat atttttcagg cgctgcattc
    31141 aaccaatttc gcgcttcagt aaactgatgt aattgcatat tcgcttgtgc agcggcaata
    31201 ttggaacgat ataattcgaa tgaactatta acctgtgcat gtgtatgatt ctgaaaaagt
    31261 atcagaagca aaatgcagta aataggagca attgcatgaa ttgaaattcg cctcatcata
    31321 atgatcctgc aaatttaatg caaggaatta aggacatcta ttgaaattta gcgcagtaag
    31381 gtcacagtgc cgcgcagcac aaagacttca ccgggttgat tggctttctc gtaactcacc
    31441 atccacacat acatgccaat ttcctgaggt ttgccgtcgt aatttccatc ccagccacgg
    31501 gtaacatcgc tggtagcaaa tacctgctga ccccagcgat tgtagatttc gaaagagaaa
    31561 atcggataaa aaatgggatt caggatgtta aattcgtcgt ttagtccatc gtcattagga
    31621 gtaaacgcat tgggtatgat cagttcactt tcttccatga ttacgactat gctatccggg
    31681 gctgaaacac agccatgttc ttcgccggtt acataaaaag tatttgactc cccaacactg
    31741 taagcaagtg tggtggcaca gtttaaacaa ttaacagcat cggccggtgt ccaggtataa
    31801 gtgacgtcat cacctgtaat agtttcaacc tgaagaagag caagtgcgcc atcatttaca
    31861 aaaacggtat caggaaatgc atttatcaca ggcgcctcat aaacaaatgc ctcaatcggt
    31921 tccgaggtac ccatacatcc atttccatcg gttactgtaa caaaatacat gccggttgaa
    31981 tcaacagtga gcgtattcgt acctgttgct aatacactgt catttgcatc tgtccataca
    32041 taactgtcat atcctccggg tgctgtcatt gttgtgaggt agcaaccttc aaatggtcca
    32101 acttcaataa ttggagcggg atttacggtt actgtttgcg aaacggtatc ggtcatatcg
    32161 catgcatcat ggatcactga tgtgtaagtg gtggttgtag ttggcgctac ccaatgtatt
    32221 ggttctacaa cacctcccgg caaccattcg taggtatatg gtggaacgcc ccagacgccg
    32281 gatgcaaata aatctataga atcgccttcg caaatcacaa aatcgggaac tgtagatgac
    32341 tgaataggat tttcttccac tgtttttcct tcaatggtaa tcacccagct tccggtaggc
    32401 atgaagtgac ataaaatact gcacggtggt tgtgtacatg aacagtgata agtacgcatc
    32461 tcgaatgtga aattataatc ggcacattgc ggtggaatac aggctatagt attaaacatg
    32521 ggcagatcaa ttccataaca agttccggca ctatcgccca ccggcggaag acaagtccag
    32581 aatgaggctg gtcccggtga gtcatcacat attcctaaaa tgcgaaaagc agcttcactc
    32641 attaaacagt cggtgacgaa aaaacatccg aagttttgtg agtaataagt gccatcaaaa
    32701 tatgctgcag tcaatgtagt ttttccggga acagtaatgt ccagtgaata gctgcaatag
    32761 tcagcttcat tccagcaaag tgcatcatat tcaaagccaa tatcaccggc ggtataagta
    32821 gcctcgccaa ttaatgttgg atcaataaca accgggtatt ttcgttctga atttattagc
    32881 cattttacat taacgcgcat acgtaaaata tatccgccat cctccggaat taattggtat
    32941 gctatagcat caacctgatc ttgcttatga tatttacttt tgttttgatc gagtattaca
    33001 ggacgttcga tgcgcagcaa attcaatccg aaaggatttt tcacaacaac atttcctttc
    33061 cagtcgccat tggataaaaa atatccgtca ccatcttcgt aaacgaattg ataccccggg
    33121 ggaagtgcaa tatggtcgtc aataaccagc cactcagcat ccgggtcgac gacctctttt
    33181 gatttgatga tgaaatttgt ttttattccg gcctttctga aagcgatctc accatcaacc
    33241 ccggagaata tatcagtgat ccatgtgcca tcgcgcccta ccgtgtaatt acttgtattg
    33301 taacgttgct ttgcagtagt tgtatttcct ttttcaaaat acatttccat atcatgattc
    33361 atacgaaaat caagatctcg aatatgtata gaggtgaaat tgttgatgaa atcaattgaa
    33421 gtaggagttg gttgtgaggc agcagcaaat acgccttcgt taattgtagg tagcaataaa
    33481 taatctatag cacgcaaata tccctgagca tcggtgtaat taattgcgcc gtatgcctgt
    33541 tgtttaaaaa atgattgtcc gttggtgcct tcctttatat aataccgtga atccagtgtg
    33601 cgcttttgta caagctctat ggctcccttt ggtgcattaa caggtggtat gccaaagtcg
    33661 ggatgcgatt gaaatgcttc gggcgtgttg ttaatcagct cctgcgtaat atgttttgac
    33721 ggaatttttg ttttgccatt cgtgatcgac acatagtcat tctgcttagg ctgcatttgc
    33781 tgagcataca gcattacgcc ctgacaaaag aggagtaata gaatgacttt tttcatacct
    33841 gtatcaaact acactgcaag gcaatatcaa cgcactaacg tgacattccc ctgcaatgtg
    33901 atcgtatttc cattgccgcc ggtagcgctt accacataaa cataagtgcc catttcctga
    33961 tcctgaccat ttgctttacc atcccatccc aatgaaccac tgttgagcac gccaagatca
    34021 tttgttgtat atactaattc accccagcga ttataaatat gtatattgat atcaataact
    34081 gctgcgccgt agattgtcag aaaatcattc acaccatcac catttggcga gaaggcattc
    34141 ggaatattta caataggggc acaattatca atcacttcac ttacatcaat tgattttgta
    34201 atgtcacatg caagatcagg tgcattgatt gttacaatat aagttcctgc ggcattaacc
    34261 tctattgttt ttgtggtttc gcctgtattc cacaaataag tagcaccttc atgaccggca
    34321 tcaatgatca ccgaaccacc aatacaatat gatgcagtat ctgattctgt tgatggatat
    34381 tctacaaaaa cggttacggt atcataacta ttacaacctt cttcatcttc ggcagtgacc
    34441 acgtaagtac ttgtggaatc tggtgttaca gtgataataa aatcatcacc gattacttca
    34501 ccggtttctg catcagacca ttcgtaatac ggcacgccaa ccgtgagcac actaaaggtg
    34561 gcactgcttc cttcacaaat agttttgtct tccgattgct ctacaggaac agtttcaatt
    34621 acattaatat tttttgttcc attgccactg ccacattcgt tcagtgcaaa aatgcttaat
    34681 gtatacgtac ccgcttcatc ccaaataatt ccaactacgt tgttcgaggt atcaactatt
    34741 gttcctccgg ttaatgacca gtcgtaatat gttgtcagga aatacggaac ggaataccag
    34801 gttgtatcac cggtacacgc tttcggagga cctgaaatgg taactattgg ggcatctata
    34861 atatggaaag taacgtattc actccatggt cccggacagt tgtaatttat ttcgcgaaca
    34921 cgaagcgtaa catcacctgt tgcacctgtg aagtcaataa atattgggtt gaaaggccag
    34981 ataaatggtg ttcctgctcc gaactgaaat tgataatcgg agttcatatt atagggaacg
    35041 atcgttgcat tgccaacagc gccttcacac aataaggtat cggaaggaat taaaaccgga
    35101 gcattatctg caggtgcacc aattgttaaa tgaaccagtg tacctaatgt attttcctgc
    35161 ggtgttccac agtttgcaat tacacgcata taccacatac cggcgtcgag ccccatcgct
    35221 acgagatcaa aatatcccgg tatttccaaa tcaattgttc ccgattctgt atcaatggta
    35281 aatcctaatc cacacaaatt agcctgagtg aaaaacatgg aatttaatac ttccacgcag
    35341 aaggtatttg tatcgcaata cgtttctata tcatcaaaca cattgacatc ataggttata
    35401 gttatagtaa ctccggaatc ttctgtgata catgcgtaaa catcttcaat atcatttgtt
    35461 tcgatatcac actcctgaat acagaccgtt ccgtatgccg accctatgga tgctggtgta
    35521 ctcgagacga cacggatgta atagttacat ccaggcggca ccattggtac gatacctgac
    35581 acagatcccg gtggtgagcc gagggcaggg tcatacgttg cgttgcttgc aaaggtgcct
    35641 atcacagaat aagtatcgaa gctgccggtt gaatcactca actgcgcagt ataaacattc
    35701 cccgggaagt atactcccgt tgagaaaaat ggcacatcga tcacactgtt gacacacacc
    35761 gcattcgagt cgtatgtgac gacaggttgt aaggtggtga ttatattcgg acaactttga
    35821 atcgtgaaac attcactcgc ctcacctgta atttccggta agggcgacgt tcggctgagc
    35881 cttaccttat agcaggatcc atcgggaaca cctccgggaa tcacggctgc aatggcacca
    35941 atcgtgtctt cagcaaaaat ggtaaatacg cccaaacttg ttggcgcagc aaaaatcccg
    36001 gctgcatttg acagttctat ctcataaata ccatcgcata atgggtcagt cagcgaccaa
    36061 ccaataatga gtgtgctcaa cttacatacc ggatcaggtg aaataaagtc gatattgata
    36121 tctactggat gtatagcctc atcataagta cctactgcaa tgatatcatc tacggcaaat
    36181 gacatcgtgc ttggaccacc gccattgtcg tttacccatc tgaatgcaaa ccggagattg
    36241 gcaacttcct caaatgcagg atcggttata acttcatatt tccagagggt ttgaccactg
    36301 tataacgcca tccccaccgg agtccaaggg ccgccatcag cactataata cacctgacca
    36361 tagtcgccgg caccaccctc gcatagccag aaaaaagtga attcgatatc gatgagaccc
    36421 agtgtacaaa agcctcctga catttcagca aagttgtcag aggtactggt tgggtcataa
    36481 ctggcacagg taattgccgg agcaccctcc tcatcataaa tgtgcaaata ggtacttgta
    36541 ggtgcgccac ctatggttcc tgattctgta aggtcttgag tggtagtgtt cggataccca
    36601 aatccaccgg tgtaactgtt gttaatgacc catttattgg ttccaacagc cgcgccacct
    36661 ggtcccggtg tgtttaaaat aaaggaaccg gtaggaacgt taaagtcttc agtaaatagc
    36721 tgaaaggtct gtcccgcaag ggttgcagac atgataagcc ctactatgag tgtgtataat
    36781 ttggtcatag gcgaaatcga ggactactaa gataaacctt attatagaaa aacccaattg
    36841 aaccgcacgg tatcattgag atcgtgtaat tatttgagac tcaatcactc tttcaaacac
    36901 cttatcttcg ccctgtgcgc gcattgaatc gtcttgccac ccaaaccgcc atctatggca
    36961 tgagtaccat tctggtgcga ttgctcaatt atgtgctggc tcccttgcac acgcgcgtat
    37021 ttaccaatca ggctgattac ggtatcatct ctgaaatgta tgcatatgtc acatttttaa
    37081 atgtggtttg catgtttgga ctcgaaacgg ctttttttcg tttcgcctca aataataatc
    37141 aaaattcgct cgacaaaaga agtgtttttg gctcatcgca aatatcattg atcgtttcta
    37201 cagcattaat aacagctgca ctcttatttt caagtaatga tattgcctcc atgcttggat
    37261 atccgggaaa gggaatttat gttacatttt ttgcactcat tatagcattc gatacattag
    37321 tgaatattcc ttttgcccgt ttgcgactgg aaggaaggcc ctggcgatat tttgcaatta
    37381 aattgacgaa tatcattatc aatgtttcat tcaatttctt tttcctttta ccggcactga
    37441 gaaataatta tgacatgttt tccggaatag gatacaccta tgatcctgat tatggcgttg
    37501 cttatgtatt tctagccaat ctaattgcta gtgctatcac tttcgccata tttattcctg
    37561 gcatgttgtc attgaaattc gatgtacaag tatggaaaaa attgatggcg tatggattgc
    37621 ctttgatcat catcggtctt gcgggaatga tcaatgaaac tctagataga gtgctcatta
    37681 aatattgggg ttcaggcaca atagaagaga acctgaaaca agtgggtata tacagcgccg
    37741 tgtataagtt atcgatcttt atgacgcttg cggtgcaagg gtttcggatg ggagcagagc
    37801 cctttttctt tagccagtcg gcagaaaaaa atgcgccttc tttatatgcg ttgatcatgc
    37861 gctattttgt tattgcatgc tgcctgatat ttttgggtgt aggaatgttt cccgacttat
    37921 ttaaaattat catcggctcc gaatatcact ccggattaca catcgttccg atattattat
    37981 tggcacaact ttttttggga atttattaca atcaaagtgt atggtataag cttaccgata
    38041 aaacgtcttt tgcaacaata atccccgtag ccggtgcact cattacactg ggcatgaatt
    38101 taattctgat acccaaattc ggatataccg gtgcagcttg gtcaactttt gcgtgttatt
    38161 taagtatggt ggtgatgagt catatcattg gacagaaaca ttatcacgtg ccctataatt
    38221 tgcgaaaaat aattacctac attgtagttt ctgttatact gtgctactct ggattattac
    38281 ttatgtcggc aacagaaaat attgtcgtca ccattgttgt aagaagctgt ttattcgggc
    38341 tatttgtcgc ctttgcatgg tggcttgatc tgagtaaact tttgagaaga aataaatctt
    38401 aatattattc atgttgatta aaatcgtcaa tacatcaaat aaccccgatc cgacttatgc
    38461 cactgcgcac gctgccggta tggacatacg ggcttttgta gatgcaccgg ttaccatact
    38521 gcctatgcag cgcaccctga ttccaaccgg attatttata gagctgcctg aaggatatga
    38581 ggctcaaata cgccccagga gcggattggc tataaacagt ggcatcacta tgctcaatac
    38641 ccccggcact attgatgctg attaccgtgg tgaaataaag gtgattgtaa ttaatctggg
    38701 agatgcccct tttatcgtta attcgggcga taggatagct caaatggtca ttgcgaaata
    38761 cgaatctgtt acctgggtga atacagacaa gctttctgag tcggaacgcg gtgcaggcgg
    38821 gtttggccac accggataag taaactcctt gttgtcaatt cagcatccac atgattcatc
    38881 attatcttta ccactcaatt ttaatagtac aatgaacatc atcattccaa tggccggcat
    38941 gggaaaacgc atgcgaccac ataccttaac tgttcctaaa ccattgattc ctgtcgccgg
    39001 aaagccaatt gtgcagcgac ttgtagaaga tatagcccac acaaccgacg aaaaaatcga
    39061 caacatagcc tttgttgtgg gtcgatttgg caaggaagtt gaggatatgc tggtaggcat
    39121 agctgcgaaa ttgggtgcaa agggtcatat ctgttatcag gatgagcctc ttggaacggc
    39181 acatgccatt atgtgtgcta aagattatgt gacaggacct accatcattg catttgccga
    39241 taccttgttt aaaacaaacc tgaaaataga tactaccatt gacggtatca tttgggtgaa
    39301 taaagtagaa aactggaaaa tgtttggagt ggttgaaatg aatgccgatg gttatatcac
    39361 caattttgtt gaaaaaccgg atcattacgt ttccgatctg gcaatcattg gcgtgtatta
    39421 tgtgaaagat ggcgatttgc tgaggaatga aatgcagtat ctgctcgata ataatatccg
    39481 tgaaaaagga gaatatcagc ttaccaatgc tttggagtcg atgaaacaaa agggatatcg
    39541 cttcagcccc ggaaaagtag acgaatggct cgattgcgga aataaagatg ctaccgttta
    39601 caccaatcag cgtgtactgg agttgaaaaa agatgcagaa acgttgatct ctccttcagc
    39661 gaaactgatc aatagtgttg taatagaacc ttgctttatc ggtgaaggtg cagttattga
    39721 gaacgctgtg atcgggccgc atgcatctat tggcaaaaat gcggttgtta aaaatgcgtt
    39781 aatacgcaat tccattgtgc aacaatcagt tagtatcacc gacaagtgta tcgccaattc
    39841 aatggtggga agttttgctg ttattagtgg aacacctgat gacctgagtg tgagcgatta
    39901 cagtactatg gcatgagaaa aaaattacga cttcaaattg gattatgcgc gttaaccttg
    39961 atgtttcttg cggcttgcaa cgggcctaaa gaagcacaac aacaaacggg aagcgtcgta
    40021 aatgtcgggt cctctgcatc gcgaccaaaa aaaggaattg atactgctga actcgagcgt
    40081 cagtatattg atgcatgtaa gtttgttgct cttgaagatt ataataaggc cgttgatatt
    40141 tttaataata tcgtaaagat agatccgaat aatgcaccgg caatgtatca actcggtaaa
    40201 atctttgcca atcacggaca aattggagat gcattagcat atagcgaaaa agcagcttcc
    40261 atcgaccccg gaaatcgcga ctatcaacag caatatgccg gaatattgca atatagcggt
    40321 cagggaagaa aagctgttga tgtgtatctc ggcatgatca aaaatgaaat tggcgatgag
    40381 gatacttatt accaattagc cgcaacgtat gaaaaattag gcgacaggca acaggctgtt
    40441 tctacgctac ttgatctgca caaaataatt ggcgatgatg aacaggtaat gtttgaaatt
    40501 cagcgactgt atgcagctgt tgaacaatac aataatgcca tttcctggtt gcaaaaactc
    40561 attaaacaaa atccggacaa tacactctat ttgcgatatc tgagtgacta ttacgacaga
    40621 aatggtcaac ctgaattagc agaaaagaca tttaatgatt tgctagctat cgacccatcc
    40681 aataccgatt ttcagttcag gaaggcctct atgcagcaaa aagcaggaga gtatgctgat
    40741 tatgtgaata cgatgaataa tgcctttgct aacccggatg gaaatattga caccaaaata
    40801 ttttatctgg tactttttat agattcgatt ggcaggcctg aattcaaaac aaaggattta
    40861 gtgtttaatt ggtcgaaact gctcgtagaa gcacatcctg atgatgcaaa atcatatgca
    40921 atgcgaggag atttccttta ttacgatgag caattacagt cggccgcaaa tgtgtacaat
    40981 aaatccattg ccttgcgcaa cgatgtttat gatgtgtggt tgaaattgtt ttacatttat
    41041 tccgacttgc ataaaagtga ttcgttggca ttggtttcca atatggcgat tgaactatat
    41101 ccgaaccaac cgctgagtta ttattttgca ggcattgctg caaatcaaca ggataaattt
    41161 gaagatgccg taaaagtact tagacgtgga ttgccgctta ctatctcgaa tatcgactta
    41221 agatccggta tgttttccgc acttggtgat gcatataatg aactgaaaaa gtacccggaa
    41281 tccgatgact cattcgagaa tgcattaaac ctcaatccta atgatgttta tgccttgaat
    41341 aattatgcat attatttatc gctgagaaaa gaaaaactcg aaagagctgc agaactggca
    41401 aaaaaagcca caacattgca gcctgagaat ccatctttcc tggatactta tggatgggtt
    41461 ttatatcagc aaggagatta taaaaatgca gctgcatgga ttgaaaaagc catgcagaag
    41521 ggtggtgata aaagtggcac ggttatagaa cattatggcg atatccaata tcgcttgggt
    41581 aatgaggcta aggcgatgga atattggaag aaagctaaag cagcaggtga tgcttcagat
    41641 atgttggaca aaaaaatcaa tgataataag ttgtatgaat aaaaatgcat atttcatatt
    41701 attgattgca actttctcgc tttctgcttg cggcacatca catgcgcttt ttagtggaaa
    41761 aattaaacca atgactgccg aagagttgga taaagaactg cgcagtcagc ttattcaatt
    41821 cgaaacattt tcttctaaag ctaaaattga ccttcaatca aatcaaggaa accaaagttt
    41881 ttcagcgtct atagatattc gtcgcgattc actgatcggc atttccttgc gttttttagg
    41941 tgttgaaggc gcccgcatcc gcatcacccc cgattctata gagatccttg acaggctgaa
    42001 tcaggaatat cttccgagag attacacttt tttgcgcgac agcctgaatc tggacctcaa
    42061 tttcagcgat ctgcaaaatc ttatcatcgg caacccggta atgtacgata gcacaacact
    42121 tgaaaccgtt gagagtgaag agttttatat actaaatgcc tccaaaggtg tgtataagaa
    42181 ccaactttct atggctgccg atttcagtat cgtccgtatg tttatccagg atttgtatgc
    42241 ccgacgcaac ctgacactgt tgtatgccga atacgataaa gttaatggaa agcgttttgc
    42301 attaaacaga aatattaaac tggatgctcg cgaagatctt acagccgatg tcgtttttac
    42361 gaatgtcgaa ttcgatgccc ctttcgaatt cagcttctct gtcaacagta aatacaaggt
    42421 caactgaaaa gggcgggatc ttacctatta ttaaaatttc tttggtttgt attgttttat
    42481 cggtgagttt gtcgttgcag gcgcaaacac aggataaaaa cgctcttcaa aataaataca
    42541 ataaactgca ggacgaaatc aaagatgctg aagagctgct cgacaatacc aagcaaaaaa
    42601 aggaaaactc actcaacgaa ttaaaactgc tcaaccgcaa aatagatatg cgcgagcagg
    42661 tcatcaacaa tattgctgcg caagttgatg gtgttaatgc tgagctcaca actaccaaca
    42721 aggccatcac tgcaatgcag gcggatattg tggagttgaa aagccaatac gcgaaaatgg
    42781 tgtattacac ttatgtgaat gatcaggatt ataagccatt gcattttatt ttttctgcag
    42841 aaagtatcaa tgatgcattt caaagagtgc attatgtgcg ttcatttcat ggatatcgaa
    42901 aagatcaaat tggggcaata gaggaaatat ctgaaagcct acagaacaaa atcgctcaga
    42961 ttgaaaaaga gaaaaatgaa aaggaatctt tgttgacaaa ggaggagcaa cagaaaaaga
    43021 aactggatga tgaaaaggcg aaaaaagatc aagccgtgaa gaaattgcag acgcaagaga
    43081 aagatctgaa aaaacaaatt gaaaaaaaga aaaaagacgc tgttggatta aaaaataaaa
    43141 ttcaggccat cattgctgaa gagataaaaa aggagaagga acgtgccgct caggaagctg
    43201 caaagcaaaa ggaactggag gccaaaaagt tagccgaatc aaataagacc ggaactactc
    43261 ctaccacaaa ttcaggaagt acttcaaatg caaaaaccac caatgccgaa aagcctgcag
    43321 cagaaaaagt taatctcggt ttaacaccgg agatgcaatt gatttcgaaa aattttgaag
    43381 gcaataaagg caaacttccc tggccggttg aacgaggcac cattactgag cgttttggta
    43441 aacatgaaca tcctgtcctc aaagatgtca tagttgaaaa taatgggatt gatatcgcca
    43501 ccgctgaagg ggctacagta cgaagtattt ttgatggcga agtggtaaat gtgatcttca
    43561 atccgtcgtt ccaaaagggt gtgattataa aacacggcga attttattcg gtttatacca
    43621 atttgtcgga tgtcaccgta aaggcgggcg ataaagtttc tacaaagcaa aagataggca
    43681 tagcctggga agatccggag gaaggaaaaa cagaggtgca tctcgagatc tggaaaaaca
    43741 ccgtaatcct tgacccttca ctctggattt ctaaatgaca ttggtatagc tgaaaggcct
    43801 gatgtgacta tttcctacat gtgttgatca tattctgcat ctcggaaatc ctttgcctta
    43861 ctcaatctga atgctcattc attatctttg cagcatgatt gcgaatgatg ccgagaaatt
    43921 taaaaaggta gtgagccatg ccaaggaata tggctttgtg ttcccttcca gcgaaattta
    43981 cgatggcctg agtgccgttt atgattatgg tccgtacggt gccctgctga aaaacaatat
    44041 caaagaatat tggtggaagg ctatggtgca aatgcacgaa aatattgttg gtctagatgc
    44101 ggcaattctc atgcatccaa ccacctggaa ggctagtggt cacgttgatg ctttcagcga
    44161 tccgcttatc gataataaag attcaaaaag acgctatcgc gccgataatc ttattgaaga
    44221 atatcttgcc aaacaagatg ataaaatcaa taaggaagta gaaaaggcac gcaaacgttt
    44281 tgaaaatttt gacgaagcca tgttccgctc cacaaacgga agggtgttgg aaattacaca
    44341 aaaacgtgaa gaggtgcacg ctcgctttgt aaaagcaatg caagatggcg atctcacgga
    44401 gatgaagcag atcatcatag actgcgatat agtagatccg gaatcaggta gcagaaattg
    44461 gacggatgtg cgtcagttta atctgatgtt ctctactaaa ttaggctcat tagccgaaga
    44521 tgcggatatc atttatatgc gtcctgaaac agcgcaaggt atttttgtga atttcctgaa
    44581 tgttcaaaaa accacgcgcc aaaaaattcc attcggtatt gctcaaattg gaaaagcatt
    44641 cagaaatgaa atcgtggcac gtcaatttat tttccgcatg cgcgaatttg aacaaatgga
    44701 aatgcagttc tttgttcgcc cgggtgaaga attgaaagta tacgattatt ggaagcaaac
    44761 ccgcatgaaa tggcatcgag cattgggcac tgatgccgct atgttgcgct tccacgatca
    44821 tgataaactt gcacattatg caaacgccgc tgttgatatc gaatttaatt tcccattcgg
    44881 atttaaagag ctggaaggaa ttcattcgcg caccgattac gatcttggtc gccattcaga
    44941 attctcaggt aaaaagatgc agtatttcga tagcgagcta aacgagagtt acgttcctta
    45001 cgtggtagaa acgagcatcg gttgcgatcg catgtttttg gcgatgttgt gcaatgccta
    45061 cgatgaagaa caggtgaacg acggcatgcg ttcggtattg cgacttccgg cagcactggc
    45121 tcctgttaag gttgccgtat tgccattgat gaaaaaggat ggtcttcccg aaaaggcact
    45181 tgaaatattt aatgaccttc gatatcgtca tcagtgttat tatgaagaaa aagatagtat
    45241 cggaaaacga taccgtcgga tggatgccat cggaacgccg tattgtatta ccatagatca
    45301 tcaaactttg accgataaca cggttactat ccgtgaacgt gatagcatgg aacaggtgcg
    45361 tattcatatt gaggacgtag caaaggtcgt atccgaaaaa gtagatatga ataatgtgct
    45421 gcgaacctta gtataacatg tttgctgaat taattttccg gatcctgaaa aggatccttt
    45481 tttttgtccc ttatcggcgt tgaagagcgt ttaatgatta ttacgcagtt atgaccggta
    45541 ttatcaaatt aagtagtaat atcgtggtgc attctatgaa aaggtgtctt cctgtaatgt
    45601 tattgtcatg tatgctcgca gtcgtttctt gcgatgaaga acctatttat gaagatttga
    45661 atggctccga aaacttgaat agaaccatta aatttgacgt atctcatgta tatgctacca
    45721 gcccttattc tgatagcact gtagcagggg caaccataaa aatatatgcc caatttgaag
    45781 atttattgac agattattat cccgatgcaa gccgcattac ggactcactg ggtcatgctg
    45841 aaataaatgg attggatgag gatatgtatt atatcaaatg cacacatcct accctcggcg
    45901 agttgattga ttctgttact actcccgctt atactacttc ctttgttgaa ttgatttatt
    45961 tcatgtaaaa aaaatcgccg attgcataac aaccggcgat cttaaatgaa ttagtgtcag
    46021 ttattttgta gcaacgtttt cgaaaggaat atccaattgc aatttttccc agttcaatct
    46081 gataattcca ctgccggcat tttcacctgc tataacttca aatttgagtg attcggtaag
    46141 tgtttcggtc atagtaggtt ttacattcac tcgcaaagca tcttccgact gatcatactt
    46201 gaatgctccc cactgggtct cattgaaatt gaaaattact acccattctc cctctccggg
    46261 aattgtgaaa agagaataag tgtccttcgc caaaggttgc ccgagaatgg ttacatcctg
    46321 tgtaaatgaa atggtagtgg cctcattggc tcccgtgcgc caaacttcat tgtaaggcac
    46381 caacgcattc cagatctgac gaccttttac actcggtgaa ctgtaattga tggtgacggt
    46441 attttcacca atatttgccg tagcggttgc aggtggactg acgcggtttt ctttgttcgt
    46501 catttcttcc ttgcgctttt ctttgattgc atcctgcgat gaattttcac aggcgcccag
    46561 gaataagctt cctgcgagta agatggttgc aaattttccg gtcgttatca tattggttcg
    46621 tttcaccggc aaagatacgc aaacatagct atagcttgat cagcctgccg gccagatagg
    46681 caccaattgc agaaatgact gtcagtagca acgataccca aaccggccag ttgagaaagc
    46741 tgtagatcag taatatgatc accataaata ccgatgctac taacaatact tgtatgaaat
    46801 cgcctttatc caaggttttt gaactgtatt agattttagc tgcaacaatt atctccgcaa
    46861 ccacatgttt tagctttcac gccaaacact gttatcgaat agataccggt tccgctgttt
    46921 cgccaacttg caatttgatc ggcacttaaa taattagata aaatatcatc gggaagatca
    46981 attgcttttt ccttcatgat gcgcacatca gaaaatcctg ccttgtgaat gatgtcaaga
    47041 tatgtatcct tttgtatcgc tcctgcaata caacccgcat acatttcggc ttcctgcaac
    47101 aattctgttg gcaattgccc aacaatgaca atatctgata cagaaaaatg tcctccggtt
    47161 tttatgacgc gatacatttc ctgaaacgct ttttctttat tcggaacaag atttaagacg
    47221 caattactaa tcactacatc agctttattc gctgtaagcg gcatgttttc tatgtctccc
    47281 aatctgaatt ctacattgct caaatttaat ttctgagcat tgtttctggc aaggtctacc
    47341 atcacctgcg tcatgtcaat accaatcaca cggccatctt caccaactat agaacgggcg
    47401 acaaatgcat cattccctgc gcctgatccc aggtctacga cagtatctcc tgcctttatt
    47461 tgtgcaaatt gagttggtat cccacaacct aatcccaaat ctgcatctgc taaatagcca
    47521 tcaagtgatg tatagtcttc actcatgatt tgcaggtcaa ctgttgagca acctccggcg
    47581 ccgcagcatg atgatgcgtt ggtgcttttt gattgagtgg caatttctgt gtaacggtct
    47641 ttgaccatat ccttaattga ttgatcgttt tgcataatct ttattttatt tgatgataat
    47701 atatttcatt aatgtagcac tcgtcggaca catgcttgta aattccgatg atttgcgtat
    47761 ggattccggc gcttgtgacc tgtcttcaat tgtgaatccg agtcgttcgt aaaatttaga
    47821 tgccgtttcg gtaagcagat atatttggcg gaaaccttgt tcgttggcaa gactcagtat
    47881 gtctatcata aattgcgtac caattccgcg attatgcatc tgcacatcca ctgcaaccga
    47941 gcgcaataat gctgttggtg ctgaaccttc aattcccgca accgccatca atttttcgcc
    48001 attttttaac agatagtaag ctccttgaac ctcattcacc ccttcaacag gaagatcatt
    48061 ggcttttagt aaagcatgaa tattggcaaa gtcggtacta tgtgccttgc ttatctgcat
    48121 caacaacaat taaatgatgt aaaaaggaaa ttgaaatgtt tacgcgcctt tccaataacc
    48181 ggttcattca aacaataaca aacccttgga ccatcgattt caccttttat taaaccggca
    48241 gattttaact ctttcaaatg ctgtgaaaca gtagattgag ccaagggcaa tctgtcaacg
    48301 atatcgccac aaatgcattg tccgtccgac aaaaccttca aaatcgctat cctggcgggg
    48361 tgagagagcg ctttggcgat gtcggccagc tgcatctcat cttcactgaa ttcggttcgt
    48421 tttgtgtaag ccataattgt atatcgcaaa aatacgatta aaggtttcaa tttgccaaac
    48481 ttttgtcaga ttttaacaat agtattggct tgaaactcat ttgcggataa ttttagtcat
    48541 cctaaacagc cactacatga aacattattt gatagctact ttatgcctga cctttgccac
    48601 acagtcagtt tcagctagga tattgggcgg agagttatgg tacgagcaaa cggccgcaga
    48661 ccaatatatc atcaaattga catggtatcg cgagtatgaa cccgagttgg aacctgtttt
    48721 ctggttgggt atttataata acactgacta tacgttgatt gatacggttg cggcaattga
    48781 aatagataga tctatagttg atagcaatta ccctacttgt gtgggaatgg ttcaaaagta
    48841 tgatatagag cgactcgatt tttccacccc ggttacactt cctgaaaacc ctgatggtta
    48901 tgtaatagtg attatgggca atacccgaac cgaagaaatt tccaatatat tattacctgg
    48961 cttgcgtggc ctgaccttat acacaactgt gccgggaaac gtaaacaata gcgccccgtc
    49021 attcaataat cctcccgttt ggtacgcttg tgaggatcag tcattaaata tggatttagg
    49081 cgcctcagaa cttgatggtg atgaccttag ttacgcactc gtgcctgcct tgaatgccga
    49141 ttatacagaa cccttttttc aatccttacc tacgccacct ccattttctg aactttcgta
    49201 tttgggagct ttaagcccaa caaacccatt cccatctgcc acaacaattg aaatcgatcc
    49261 atctacagga atgctaacgg gattgccaac aactgagggc gtttatataa taactgtaga
    49321 agttactgaa acgcgattag gcattgagct aagcaaaaca agacgggata tcattttaat
    49381 tgtagaggat tgtactccga ccatggcaga agttgacctc ggttccgaaa tctatcaatg
    49441 taacgatacg catgtatttt tcgaatgtga aacagagcca tgggccggct attattggga
    49501 ttttggtgac ggtataattc aaacatggga tatttatgag catacttatc ctgcacttgg
    49561 agattatgaa ttgatgtaca ttgccaatta tgatttacca tgtgcagata cattttattt
    49621 gcctgttcat ataagagaat tatcagagga tgtactgagt accgaccttt taggaaatcc
    49681 ggaaaattgt ttttatgaca cgattcaatt gattggagag ccaggattcg tagctgaatg
    49741 gcaggatgta aataccggtt taataattgg aacaggatca cctttaccgg ttactcaatt
    49801 tagtggagac ttgctggcta cagcatatta ttatgacgat gatttttgct ttgcatcgga
    49861 tactgtttca gcctatttct tcccggataa tacacctttg gttaaaataa atcaggataa
    49921 cccccaatgg tttttgtatt gtgataatgc ctttgatccg ttcgtggaaa cattgcaatg
    49981 gtttaaagat gatatgccta ttcccggagc aataaatcca aacctgccac taacttcatg
    50041 cggttcctat tatgccagaa ttactgatac acatgcatgt gtttttgaaa gcgaagcaac
    50101 ctattattgt aattctgaaa ttgagggtgc ggagacttta ttttttgaag tttcacctaa
    50161 cccgtcatca ggaagttttt cattaacact ccccaatacc gatccggtta caatccgcat
    50221 ttacaataac cttggagaaa aggtgtatca aatagaaagc gctggaaatg cagtaattaa
    50281 tattaacact gaaagtcttg caggcttgta tcatgtaata attgccggcg aaacctatgt
    50341 aaaggaatta cctgtaatga tcaattaaca ttcaatctct ccaactcttc ttgcaatttt
    50401 tcccaatcgc tcattttgga ttcatgcgat tgcttggtgg tgttgtactt ggtaaaaaaa
    50461 tcagcagtat ttgaattgtt atagaagtcg gcactattca ttgttgcctc catcgttttt
    50521 atctctgcct caagggtttt gatttcctgc tcagattttt caatagcttt ttttaccctt
    50581 tgaagttctt tctccttttg ttttttatcg gcatattgca ctttctgttc ttgcacaacc
    50641 tccggaacag cttctttttt cttcggttgc ttttctgtca tctcaagctc ggcaagattg
    50701 ctgatttttt tagcatccag ataatcatag atgtcgccga tatattcttt aatcgtttta
    50761 tccttgaatt caaatactct attcgtaatt ccctttaaaa aatctctatc gtgcgataca
    50821 acgatcaccg tgccatcaaa atttgcgagt gcatttttca atacatcctt gcttcgcata
    50881 tccaagtggt tagtaggttc gtcgagtacc aataaattat atggttcaag caaaagcttg
    50941 cacatggcga gtcgtgattt ctcaccaccg gaaagtactt ttactttttt aaaggaagct
    51001 tctcctccaa ataagaatgc acctaacaag gttctgatat gtgagcgaat ttcacctgct
    51061 gcaactttat ctatcgtgtc aaaaacagtg tcgtcgccac tcaatgtttc cgcctgattc
    51121 tgcgcatagt atcctaaatg aacattgtgg ccaatagtga gctcgccggt atatttttca
    51181 atacccgcta tcatttttga taaggtggat tttccttcac cattctttcc tacgaatgca
    51241 atcttatctc ctctgtgaat ttcaaaatcc aataagcgca acacttcttt ttcaccatac
    51301 gatttcgaca cacctctccc ttctaccgaa attcggccgg agcgtggtgg ttttggaaaa
    51361 tagaagtgga tggtagcagt ttcttcgtcg tcaacttcca cttcttcaag cttgtccagt
    51421 tttttaataa gcgattgagc aaatttcgct ttgtttgctt tcgcacgaaa cttttcaatc
    51481 aggcgttcgg tatgttcaac atatttctcc tgattttttt gtgccgcttt ttgttgatcg
    51541 atgcgctctt ttcgcaattc aacgaatgta gaataagcgg cattgtaatc atatattttc
    51601 tgactaacga tttcaatggt tcttttagtt acattatcca aaaatgttct atcgtgcgaa
    51661 accaaaatga tggcgccggg gtatgtttcc aaatatgttt ctaaccattg aatactttca
    51721 atatcaaggt ggttagtagg ctcatcgagt agaaccaatt ccggtttgct gaggaggatt
    51781 ttcgccaact cgatacgcat ttgccagcca ccgctgaatg tttccaccgg tttattgaaa
    51841 tcttcccgca tgaagccaag ccccatcagc accaattcca tctgcttgtc gatagaacct
    51901 acatctataa tctccaatct gtgcacgatc tcattcattt cctcaatcag atcgtgatat
    51961 gaatcgcttt cataatcggt tctaactgtc agatcatgtt ctatctcatg taaacgcttg
    52021 tcgaaagcca atgcttcatc gaaggctgtg cgcgcttctt ccatcacagt cttacctttc
    52081 attttggcaa ggtcctgatg taagaaacca atcaaactat cttttggaag aattacctgt
    52141 ccggaatcag gctgttgcaa gccactcaaa atcttcatga gtgtcgactt accggcgcca
    52201 ttttttccca ccagaccaac gcggtcgtta tctttcagcg taaaggaaac atcttcaaat
    52261 aaaaattcac ctccgaatga aatggagagg ttctgcactg caatcatggc gcaaaggtaa
    52321 ccataagcgc attaacgcac taaacgggct gaggcattta caccggtctc ttggatgctg
    52381 agcaggtagg tacctgctgc aattgaaggt aattgaagtt gaatggaagt agctccggcc
    52441 tgggtttgga atggataatc cgcaatgagc cgacctgtta tatcaaatac atgcaaggtc
    52501 aaactacctg tataaggaga aaacaaggtt aatgcaagat ttcctgtatt ggcgtcatat
    52561 tcattgatca ccaatgcatc agtattgcca gagctttgaa tggatataat attggaatag
    52621 tcagtagtac catcttcgtc aacctgcttc aaccgataat aatatacatt acttgctatc
    52681 tggtcgtctg agaaagaata ttttgtttgc caaggatttg ccgcttcaac cattcctatt
    52741 gaaacataat cacgaccatt gcttgatctt tcaacaaaaa acgttttgtt ttctaagctt
    52801 gaagaggctt cccactctaa gatatttaca tcttgcaaag aatagccgct aaagcttatt
    52861 aattcaattg gcaaggtaac acagttgttt attaccgtaa cattatctgt agcaacattt
    52921 ccgcaagcat cggtcatggt aactgtatac gttgttgttc ccattggaaa agtaacatac
    52981 accggactag tggtataacc tcccggcgac caggtatacg tataaggtgg tacaccataa
    53041 ttaggtgttg ttgtcgataa ccagccggat tgtaaagtgc aatctaaaac agtataagca
    53101 gtagctcctg ctgcgaggcc tgtggtggta actgtttctc cttcaacagt aattgttgcc
    53161 agatcaagat gtgcgcacgt tgtgacacat ccaccaggtg ttacacattg atattgatta
    53221 tgataaatag taaaagcaat gttaaatgaa gcacattgtg gtgtaaaaca agatacgagc
    53281 gctgcagtag tagcatcatc aactgttgga ttccaagtgc cggcagtatt acatgcagga
    53341 caaacccaaa cacctgcagc agacgggctg tatccgcagg aggtgccaat gcgtaattga
    53401 aggaatttta atttacaaaa tggcgaacat gctacaccat cggcatcaaa ttcccaatgc
    53461 acagatgaac ctgttaaagt tgcattagca ggtgtaggca cattaacaac gacattacaa
    53521 aaagtaggag agtcgtctgt tccgcttgtg cctgtccatg ttgtggtagt gccgtataca
    53581 gtcggatcaa tggtaatcgg aaattgacgt tgcggatcgc gcaggtacgc catgtcggca
    53641 accaatttta aggttacggt tctgccattt tgtttcacgg tgtaaccaat aatatccata
    53701 gggataacaa agtcaagagt gtcattcgga ttgctgtcgt acatcaccgg agctttcatt
    53761 ctcgccattt caaatccatc ggcattttct ataaccagat cgccaaacca gaatccgttt
    53821 acattaaatg taccttcata ttgatctgtg ctgatggtgt atccttcagg aagatcaaat
    53881 tcatcgcgaa tgacaaaata tttttttgat gtatcaatcg cattcaaatt ccaaacagta
    53941 tatcctgatt ttatttctgc aacattaaat cgcaattcct gatcaattcc tggaaaagca
    54001 tctttaacgg cggcgccatc agcaccaaca gaataaacag atcggttgat ggcaataccc
    54061 ggaatattta aaccaatttc agacgttgca tatgcgctga tattttgatt aaatcttaat
    54121 tctgcaccgt cttgcatggc tatggcagta tatccttcta ccatatttaa tgtggtagga
    54181 tttgtttgat gtaatcccaa ataaaccccg ggtaatgcag tagatggttg aagcgttgga
    54241 tctatagtaa cccagtttcc tttattatcc ttgtaattta aatcctgata tgcaccttgc
    54301 gaataaaatt tacttccatt ggttcccttt ttcacaaaca tgcgcgaatt ctgagttcgg
    54361 tattgcaata attcaatgca atcttcacat ggtgcattat atggtaatat gccaaactcc
    54421 ggatgggtat aaagtgaatc tggtgtaagt gaaaataatt ctttaggtgt atcggatact
    54481 gcagtattga aagttccgta attatatgtt ttcacccctt cggcatgaaa ctcatcttgt
    54541 gccataccgg tacgtagcat ggatgaacta atggtaataa ccgaaaatac agcttttaat
    54601 attacccgtt tgcagttcat gatgaggatt aattatacct ttaatcttag attttgcctg
    54661 aaatatgagc agaagctgat attttgagta tgcaaatata agttttatgt cacaaattca
    54721 aaaaagtaca cttaaatttt taaaagacct tgccgcccat aacgatcgcg tatggttcga
    54781 agacaaccga accatttacg aggctgccaa gggtaatttt atcgatttca ccgccgcggt
    54841 tttggagaaa acagcctcct tcgagcctgc aatggcgggt atagatgcca aaaaggctgt
    54901 ttttcgcatt tatcgggatg ttc
//
LOCUS       DAOQOU010000003        51526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_252_length_51526_cov_5.984885, whole genome shotgun sequence.
ACCESSION   DAOQOU010000003 DAOQOU010000000
VERSION     DAOQOU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 51526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51526
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_252_length_51526_cov_5.984885"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1050
                     /locus_tag="PLM90_00405"
     CDS             91..1050
                     /locus_tag="PLM90_00405"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMZ87853.1"
                     /translation="MTILITGACGMVGSHMIDMYYAQGITDLVGTFYKPTTDMEDIHG
                     KARLVECDVRYYQRVHDIITGTKPDKIFHLAAQSYPTVSWHRPQETMDTNVTGTINVF
                     EAIKAVRKTDPDYDPVVVVACSSAEYGASLTPENTPVKEEAALLPLHPYGVSKVGQDL
                     LSFQYWQNDKIRCIRARIFNTTGPRKTNDVVADFTSRAVAIERKKANTLTVGNLNTRR
                     AITDVRDLVAALMLLSDKGNWGDVYNISGNKVYTISEIIPIIENYMGMSLTYEVDPKL
                     LRPTDEPIIYGDSTKLKNDTGWEQKYDLATTIRDMIDYLRSKP"
     gene            1053..1910
                     /locus_tag="PLM90_00410"
     CDS             1053..1910
                     /locus_tag="PLM90_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HMZ87854.1"
                     /translation="MMLNRTRLFDLDLINDHNFDAVISSMMDFSHEYDPLSDKLPLLF
                     TPNVDDVVKLNEKQYADLASVLRRSMYCLPDGQPIIWVSKLFKGRKLKRRLPGSELFP
                     LLWKEVISKQKRVMLVAPSEQVGELLKKEYPAMVYYVPPFFDVQDEVQLKKVQQEAAA
                     IYDSIMPELVFIGIRYPKQNHIALGLIDHHNRTYATPRGKEEKVGIRSYQLYGSNSAQ
                     PHALHLLMGASYEFYLGLKNRAPQFWQKIGMEWFYRFTQEPGRLFKRYFIDDLQFFPI
                     VMKEFFKSK"
     gene            complement(2570..3040)
                     /gene="tnpA"
                     /locus_tag="PLM90_00415"
     CDS             complement(2570..3040)
                     /gene="tnpA"
                     /locus_tag="PLM90_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HMZ87855.1"
                     /translation="MSSTYSQIYIQIVFAVKHRECLIRYSWEEELYKYITGILTNKQQ
                     KLLAINGMSDHIHIFIGMRPSCCLSDLVREVKKASTEFIKENNFTKSKFSWQEGFGAF
                     SYSHSSIDNVIKYIMNQKEHHKVKSFKEEYYAYLKEFEIAYKDEYLFEWIDTES"
     gene            complement(3341..4567)
                     /locus_tag="PLM90_00420"
     CDS             complement(3341..4567)
                     /locus_tag="PLM90_00420"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ87856.1"
                     /translation="MKAVIPPMKYILFILFAAFCQPIHAQWTLLPVEGPQCCSQIWFS
                     DPTHGYYSGGDSTLLDSWDGGMIMETFDGGISWDTLFDSECDENYPFNVVFVGDVGYT
                     SNRYSNFYRTENGGKDWELIELGGTNFLYNGLQMPDDSTVLIAGYSGEIFKITDNGEN
                     ITRVLFLQPHYQSFIGMQCIGSTCYAYTNDFIFRSSNAGDTWDLIYTSVEDAIKLLFI
                     YPNHQIMMATKNADFGFRLFRTKDSCATWDTLGVMDADVFAINDMAFQDSIGYFVGYD
                     YLLKKTTDSGRTWVSIPLDTIYDIPGDLHELQLLSADIGFIAGYEGLFYSMGVPTSFP
                     TTDGKLDLLLYPNPADSYVNILLPESSDISVFNAIGQLVYQINSQQHVYIDTHSWANG
                     FYIAKSDFGAKGFIVQ"
     gene            4912..5940
                     /locus_tag="PLM90_00425"
     CDS             4912..5940
                     /locus_tag="PLM90_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HMZ87857.1"
                     /translation="MIIRSKAPLRIGLAGGGTDVSPYSDIYGGCILNATINKYAYATI
                     LPRNNGKIVLHAMDRNERIELDSISKLPIDGTLDLLKGVYNRIVKDFNHNAPLSFELN
                     TYVDAPPGSGLGSSSTLVVAIVGAFTEWLKLPLGEYDIAHLAYDIERKDLTMAGGKQD
                     QYAATFGGWNFMEFYKDDKVIVNPLRIRPQYLQELEFNIVMYYTGTSRLSSTIIEAQS
                     SNVTKKSDKSIEAMHNLKEQAYMMKEAILKGELNRIGDILDYGWKNKKATASGVTSDV
                     IDSIYESAIKAGATGGKISGAGGGGFMTFYCPGNTRYKVIEVLNTFGGEVHNLEFSKY
                     GLTSWEAK"
     gene            5925..6515
                     /locus_tag="PLM90_00430"
     CDS             5925..6515
                     /locus_tag="PLM90_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HMZ87858.1"
                     /translation="MGSQIEKIKSVIAESIRVKNDILNNPEMIAKCEEIAERIAEAFR
                     QDKKVLFCGNGGSAADAQHLAAEFSGRFYFDRPPLDAEALHVNSSYVTAVGNDYGYDQ
                     VFSRVLKAKGRQGDFLFGISTSGNSGNILKAFEVARERGMVIVAMTGESGGKMKDNCD
                     YLLNVPSNDTPRIQESHIMLGHIICELVEASLFERP"
     gene            6512..7207
                     /locus_tag="PLM90_00435"
     CDS             6512..7207
                     /locus_tag="PLM90_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ87859.1"
                     /translation="MISEAIVLAGGFGTRLQKVVAEVPKPMAPVAGKPFLHYIFDYLI
                     KNDITHAVLAVGYLREVIIDTYGDNYRSLKLTYSIETDPLGTGGGIMQACKYLQGNEA
                     MVINGDTFFDVDLHALTSFHQQQKALLSVALKRMENFDRYGTVETNDTHQITGFREKR
                     YMSEGLINGGIYCLNTAIFPEHIPQKFSFEKEILEKEIHAGRIFGMESQGYFIDIGIP
                     EDYARAQDDFKSR"
     gene            7239..7787
                     /locus_tag="PLM90_00440"
     CDS             7239..7787
                     /locus_tag="PLM90_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ87860.1"
                     /translation="MQKPVPQIDQSWTLFLDRDGVLNRKIDHDYVRNWSQFDWLPGVK
                     EALAYFATRFGKIVIVTNQRGVGRGWMTLEDLNDIHTRLKQAVTDAGGRIDGIYFCTD
                     TTDEGSTHRKPKPGMAMDAKGDFPAIDFSKSIIAGDSGSDIQFGKNLGMYTVLIAPES
                     KHDADLTVPGLAAFARLLQQND"
     gene            7948..9351
                     /locus_tag="PLM90_00445"
     CDS             7948..9351
                     /locus_tag="PLM90_00445"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HMZ87861.1"
                     /translation="MVRRYSQFLELVYFLVDIVLLNVSFLIAIFINFDRISKVSDDRK
                     FAMLLVAVNLIWFMVTTVTNYYKSEKERRTGYEHLVFRFLKVLAFQVMLTFTYLILIK
                     GYTFSRLQLGYTYIFFTVLDLGWRITFETYLKRYRARGGNYRRIIVIGTTQASLNFVK
                     EIQEHNEFGYRFFGYFDDPEKSMVEVEGSLEDVQSYCRKHNIDEVYFALHPSDMSEYV
                     DDLLNFCNDNLVRFRMVPEFSSYISKQFKKVNIEYYGSTPILSIRPEPLDNLYNRLLK
                     RLFDIAFALLFFIIIGWWLFPIIALFVKLSSTGPVFFIQKRSGEGNEEFWCYKFRTMK
                     VNAEADTKQATKGDPRITRVGAFLRKTNLDELPQFINVLLGDMSVVGPRPHMLKHTEE
                     YSKIVNNFMVRHFVKPGITGAAQANGYRGDTTDPVMMEKRVQYDLWYLENWSFWLDVK
                     IVFLTVWSMLKGNENAF"
     gene            9341..10969
                     /locus_tag="PLM90_00450"
     CDS             9341..10969
                     /locus_tag="PLM90_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87862.1"
                     /translation="MRFRLLTLLIAISTAASAQQTFLPLNRNIYAWYEPAVNEQGVLF
                     HTAIKPWRMQELQQVVTYDSVFHLKKVSDDKFIGKVWNFFGYDTILRVNTEDFHLRVD
                     PLINFAGGMDIENGGKGLWTNTRGISVMGDIGPERKVSFQATLRENQAKYPDYLVPWI
                     KYYNVVPGQGKVRSFKNSAFDYANATGYVSYTPSKYFNFELGHDKNFIGDGYRSMLLS
                     DNALYYPFFKITTTVWKIKYMNLYTEFDDAYNTLDDSVMVVTGLTRKFGSFHYLSWDV
                     FDWVQVGLFEGIVWPANDSTRYRGFDVNYINPIIFFRPIEFGLGSPDNALIGANIKFK
                     PVNNAIIYGQFLMDDLDVAAARGGEGYYRNKFGIQGGFKIYNPFKINNLMIQAEMNQA
                     NPYTYAHKEPVQNYAHYNQPLAHPLGANFREFIGIINYRFFERFYMEAKVQSAVIGYD
                     QHAGDHFGSNIFISDYDIPGFPDSYGNYIGQGLKTTIRSADLRGGFLINPSINLNLEG
                     QIFIRRFANDQEKINTTMFMLALKTDLFNSYYDL"
     gene            11017..11088
                     /locus_tag="PLM90_00455"
     tRNA            11017..11088
                     /locus_tag="PLM90_00455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11050..11052,aa:Arg,seq:cct)
     gene            11105..11176
                     /locus_tag="PLM90_00460"
     tRNA            11105..11176
                     /locus_tag="PLM90_00460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11138..11140,aa:Arg,seq:ccg)
     gene            11342..11848
                     /locus_tag="PLM90_00465"
     CDS             11342..11848
                     /locus_tag="PLM90_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87863.1"
                     /translation="MSRRYIALTLLVAFTLKLCAAWLIHLWQIQSIHAYQQEKIESGD
                     FNGETIAHLVVDREMISWVEENEFVFEGYLYDVINLVEHDGYLSIAAIKDHKETEKDH
                     NYKQNQNQQQKEEGGRNELQTTTIPFTIPAYLRVIFEQSSRLLTALPVDNMQTNFTLK
                     VLAPPPRA"
     gene            11895..14102
                     /locus_tag="PLM90_00470"
     CDS             11895..14102
                     /locus_tag="PLM90_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ87864.1"
                     /translation="MKKILLMSAMLCTIAAFAQQTETTYDLNEVIVSGNRFPEERREV
                     AQKVDVIKTSTINFTNAATTAELLSESGKVFVQKSQLGGGSPVLRGFEASRVLMVVDG
                     VRMNNAIYRAGHLQNVITLDQSILDRVEIAYGPSSLVYGSDALGGVMHFHTKNPKLLV
                     DGESMASGSAFLRYATAADAMSGNFNINLAGKKIGSLTSVSYNDYNDLRSGTVENDEN
                     KNYWTRPYYVVTTNGVDELKANDDKYTQVYSGYTQYDVMQKFLYQQSAKLSHKLNFQY
                     STSTDIPRYDRLTDPLNATTGDSLRFAEWYYGPQDRMLVAYDMLYAGKTAAFDNMHVI
                     LSYQDIQESRHQRRWNKTNLQHRIEDVSVMGANIDFEKKINANTITYGLESYFNDVTS
                     SAHEEDILTGEETPLDTRYASGGATMMNAAAFASHQLKFSNNKFVLNDGLRFNYSKLT
                     ASFTDKTFFPFPYTDIDQQNTSLTGSLGLVYSPDNSTKISLIGSTGFRVPNVDDLTKV
                     FESGPGSLIVPNPDLGPEKTINADLSIVKVFKDKLEIELTGFYTRFTDYIALEPGTFE
                     GADSIEYDGVLSEVLTTVNKGSAYLYGFNVGADVNIINGLNLSSYITYTYGRIETDTT
                     PYPLDHIPPVYGRTGLKYAKKKLVAEAYALYNGWKYIEDYNIVGGEDNDQYATPEGMP
                     SWYTLNIKASYDVMEYVTIQAGCENLMDHQYRVFASGISAPGRNIFVAVRAKF"
     gene            14225..14527
                     /locus_tag="PLM90_00475"
     CDS             14225..14527
                     /locus_tag="PLM90_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87865.1"
                     /translation="MSLFSKLLGGNKGGGGGNKAQAFITQLTQDLHLSSDQVTQVEAA
                     LREFFSEKREMKQSGNKDGFKESKQDFKDDILKALSPEQQQKFMDNIQTYKQLLRK"
     gene            14580..15110
                     /locus_tag="PLM90_00480"
     CDS             14580..15110
                     /locus_tag="PLM90_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87866.1"
                     /translation="MTDLLFANVLLFGSNEPTVHLPEGLYKALLASHSGLRYIVMILL
                     ILAVVYARQTSSGKKPFAGATKKMGMFTMILVDIQLLVGAALYFNFIASQTNFKLGKL
                     KDQLSAPMFRSIALDHALGMFIAIVLIHLGYAKAKKSMNDVDAGKKQFMFYLIALILI
                     LVSIPWPFLHHERGWF"
     gene            complement(15181..15813)
                     /locus_tag="PLM90_00485"
     CDS             complement(15181..15813)
                     /locus_tag="PLM90_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ87867.1"
                     /translation="MDEAEYISQAKTCALYKKGQTIFHEHANSYGLYCLNEGKVKLYK
                     QAPDGKEQIMKIVTPGDFIGYGAMLSSQTYGVSAEVIEDAVICFIPKDSVLKIFRENN
                     RFSEGMVQLLTKTIDQTVEKMADIAYKPVRGRVAEALLLLSESYKTEDNPDGVVSITR
                     EDLASYVGTVKETAIRVLKDLKEEGLIETNNHAIKIIDPKGLAKVCELYD"
     gene            complement(15929..16852)
                     /locus_tag="PLM90_00490"
     CDS             complement(15929..16852)
                     /locus_tag="PLM90_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ87868.1"
                     /translation="MPSSINGPGKQESSRTRTQKREQFKERLMQVNRILVPTDYSENA
                     DLALVLAVDIAKQFDAELLVVHAYHMPFTDEHMPPDMVKELLAAQKLRADDDMQRYAG
                     KHRTDLKWTKVQFKAMMGFASETILDIAHTEKVDLIVVGTQGANSIEDRMFGTVTWNI
                     IKKSDIPVIALPTHNEPVSIKNVMVPFEGTDQDYDIIGYLLGFTAKYNATLHAVHFMN
                     DTNSYNKALVDKLQTGFRKEIDAERLQLHFLAEKNITEGIKKFASRNQVDMIGMVTHN
                     HGLFSTIFHMSVTRNIALYSQIPLLAYNCDK"
     gene            complement(17087..17638)
                     /locus_tag="PLM90_00495"
     CDS             complement(17087..17638)
                     /locus_tag="PLM90_00495"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HMZ87869.1"
                     /translation="MKKFLTIVCLMGSVAAFAQKGEKVERPAFPVDEETKLVTYTDVI
                     QVPGITKDSLYNVAMEWMKKFYKSPSQAIKSQSKEDGVIEIKHQFQLTRDEKGQTVKA
                     AQINYYLTLQFRDGRFKYTITKIHVEGPSYFGIEKWINEENFAKDENVTGYLVQIEDF
                     MNKLTESLDSELRPAAPKKEEEW"
     gene            18159..20114
                     /gene="gyrB"
                     /locus_tag="PLM90_00500"
     CDS             18159..20114
                     /gene="gyrB"
                     /locus_tag="PLM90_00500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMZ87870.1"
                     /translation="MEKEAEELKKNNGYSADSIQVLEGLEAVRKRPAMYIGDTGFKGL
                     HHLVYEVVDNSIDEALAGYCRNIEVSILPGNSIRVKDDGRGIPTELHKKENRSALEVV
                     MTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSTHLTAEVHREGKIFKQEYETGKPLY
                     AVKEVGESNITGTIITFHPDDTIFTVSEYRYDTLAARLRELAYLNKGIRLILTDERTA
                     DEAGNFVREEFYSEGGLIEFVRFLDGSRTPIIPEPIYAEGARDGVAVEIALQYNDSFN
                     ENVHSYVNNINTIEGGTHVAGFRRALTRTFKAYGDKEGMFEKAKVEISGDDFREGLTS
                     LISVKVPEPQFEGQTKTKLGNNEVLGIVDTISGEMLFNYLEENPKQARLIIGKVILAA
                     QARIAARKARELVQRKSAFGGGGLPGKLADCTDKDPQRGELYLVEGDSAGGTAKQGRD
                     RTFQAILPLRGKILNVEKALDWKIFDNEEIKNIFTALGVMPAEDKTLDTTKLRYHKII
                     IMTDADVDGSHITTLILTFFFRKCYELVQQGYIYIATPPLYLVKKGKDQRYCWNEEDR
                     KAAVADFSKGGSESSVHVQRYKGLGEMNAEQLWETTMNPETRTLRQVTIDSAAEADHI
                     FSMLMGEDVPPRREFIEANAKYANLDI"
     gene            complement(20188..20805)
                     /locus_tag="PLM90_00505"
     CDS             complement(20188..20805)
                     /locus_tag="PLM90_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87871.1"
                     /translation="MISFKRFLCSLFLICSLQIVQAQTQPIDPSDAETTNEESSNDDG
                     DSGDDKKNWVDNIVVGGNLGAQFGQATYIEASPIVGYRITEMLTAGVGFTYQYFSENY
                     NLATFADYKAQVLGPRVFVQHDLIYGLFAHAEFEHSWVKFTYEDPTYGEYTNDVSALF
                     LGMGYNYEVGSNSRFQIMLLYDVLHNIESIYYSPWVVRMGFNIGL"
     gene            21036..>21670
                     /locus_tag="PLM90_00510"
     CDS             21036..>21670
                     /locus_tag="PLM90_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87872.1"
                     /translation="MKLLNKEQVDWRLILFVLLLVLLPFILFADSAAKSPLPEGATDP
                     AFNIESTPDTLKEAFPALYSSLKISLATIPTDTVYVIIHPGDQLNVAGGFGLNDTLRF
                     TPYPMALSQQEVKVKPWNDLILEGWHFGTLTFEIVTDAVEYAGLAIPDISYTIEDNEL
                     PPGIVQAVDMDTTLTEGLTGIDLLFSMATIPTDTVFLIIDPDDQLRITGIPG"
     assembly_gap    21671..21770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21771..22997
                     /locus_tag="PLM90_00515"
     CDS             <21771..22997
                     /locus_tag="PLM90_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87873.1"
                     /translation="IRLSSIPTDTVFITFDPDAQLRVTGIADVPVTVIYPPTAAALAF
                     DGVSLRAYDDYIFEGDHSGTIQISIESNDPYYDAMSLPDMTFPIIDNDNPPGLTYSIP
                     DVLELSETGGELPIVVALQSVPTDTVKILVDPSLQLRITDPGVGVYMIFPPNASALND
                     HILNIKAVDDADFEGTHTGSFSFNFETIDPDYALMDIPTQTVTIFDNDLEPGIIFLDT
                     FGLAGVEGDTSLDILIKLNSIPLNPVTIELTPDVQLDLGKGPGGLVKYKFRSDSALIF
                     QTVRIKIVDDHLIEGDHKGYVSVNITTEDPYYMIMSVPTFDVNITDNDIVPVYNYNPL
                     VFNAFPSVAGDLINIQSPENGKIDVIDANGKYMFGIENGIGSTSIDTHSWPSGNYMVI
                     WRSEENAYYARFIVLH"
     gene            23075..25489
                     /locus_tag="PLM90_00520"
     CDS             23075..25489
                     /locus_tag="PLM90_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HMZ87874.1"
                     /translation="MQDHQHATSVLTCYHCGEQCKDTTFEIEDKHFCCFGCKTVYEIL
                     QDNDMCAYYDMTAHPGVQQRNKTDIRKYAVLDDQQVRANFVRFADGNQTHVTFYLPAM
                     HCSSCIWLLEHMHKLHPGIIQSRVDFPKKEVSVVFDERELALSKVAALLDSVGYEPLL
                     SMSDMDKKPEKTLQRSRLIKIGIAGFAFGNVMMLSFPEYLAGGEVTDAGLQSMFRFLS
                     LALALPVFFYCASEFYVSSWKSIKNKFLNIDVPIALAIIITFTRSLYAIFVEHGPGYF
                     DSMTGIVFFMLVGRYFQDLTYKTISFDRDYRSYFPLSVSLLKKGEEVEKPLSALETGD
                     LIVVRNNELIPADALLVKGKGQIDYSFVTGESEPIEKRIGELVYAGGRQTGAAIELKV
                     VKEVSQSYLTQLWNNIDVSRKEDERKSFVQMLGRYFALIVLIVSAIACGYWIMQGDYQ
                     RALDSLITPMIVACPCALLLSATFTNGNAIRYFGKYGIYLKNALVIEKMTKTDAIVFD
                     KTGTLTQSNTTSTEYIGEELSSVEKHMIAVVAAQSTHPYSKAIVRFLGNTMLYAVENF
                     KEFTGAGIEGMVDQVHIQIGSAKFVGVPNALQEEHGIAYIAIDNVVRGRFRFANKYRA
                     GLDDMISRLKQQYDVYILSGDNDADKEYLLQYFPAEHMFFNQSPHDKLKFIKQLQQRH
                     RKVMMLGDGLNDAGALQQSETGIAVAQSSNFTPASDVVMDVKQLINLPALFKYATAAR
                     RIIIGSFVLSIIYNVIGLYFALQGSLSPVVAAILMPLSTITIVSFTTGMTTLAAKWVM
                     GKNRAS"
     gene            25710..25889
                     /gene="ccoS"
                     /locus_tag="PLM90_00525"
     CDS             25710..25889
                     /gene="ccoS"
                     /locus_tag="PLM90_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HMZ87875.1"
                     /translation="MSVILILIIASVLVAAGFLFAFIWSVKKGQFEDDITPGIRMLFD
                     DEKYIEKSHSTNKPK"
     gene            25896..28040
                     /gene="ccoN"
                     /locus_tag="PLM90_00530"
     CDS             25896..28040
                     /gene="ccoN"
                     /locus_tag="PLM90_00530"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HMZ87876.1"
                     /translation="MDLVKVSYDNKIVKWFAIATVFWGIIGMTVGLWIALELVFPQLN
                     LGVPVLTFSRDRPIHTNAVIFAFVGNGIFMGVYYSLQRLCKTRMLSDFLSKLHFWGWQ
                     LIIVLAALSLSMGITTGKEYAELEWPIDILIALIWVVFGINMMGTIIKRRERHIYVGI
                     WFYIATFVTVAMLHIVNSLELPISLTKSYSIYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYFLPKAANRPVYSYKLSIVHFWTLIFLYIWAGPHHLLYTALPHWAQTLGTVFSV
                     MLIAPSWGGMVNGLLTLRGAWDKVREDVVLKFMVVAVTAYGMSTFEGPMLSLKNVNAI
                     AHFTDWIVAHVHIGALGWNGFLTFGILYWLIPKMWRTTLYSKSLANLHFWIGTLGILI
                     YTVPMYWGGFTQALMWKDFTPDGHLTYSFLETSTQLKGMYLMRSIGGTLYLVGVFMMA
                     YNLVKTAKQGSFANNEDVEVPEMERVVKVPKNEFWHRALERRPVQFMVLALVMVIIGG
                     VVELVPTFLVKSNIPTISAVQPYTPLELQGRDIYVREGCYVCHSQMVRPFRSETERYG
                     EYSKAGEFVYDHPFQWGSKRTGPDLAREGVGWLKKSDSWHYMHLLDPRSLSAGSVMPE
                     YSWLHENELDITTTPAKIRAMQKLGVPYPEGYDQIANDDLMKQAEEIAASLKKDDIET
                     EPTVEVIAIIAYLQRLGKDLETENALNASNTK"
     gene            28050..28244
                     /locus_tag="PLM90_00535"
     CDS             28050..28244
                     /locus_tag="PLM90_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HMZ87877.1"
                     /translation="MNFMAYLSDINGVSIYPIISLIIFFAFFIGLFVWVSRMSKSSVD
                     ECKQIPLEDNQPIHSTSNHQ"
     gene            28246..29406
                     /locus_tag="PLM90_00540"
     CDS             28246..29406
                     /locus_tag="PLM90_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ87878.1"
                     /translation="MKKLILNKHLMVALGAVLMTTPLMAQTTPAPEKNWYVHDGLFWL
                     LIAVAVILLYVIYALAEVVIWGAKKKISDRGNTTKILALVGTAVMLMSGNSLMAQAAS
                     APAAAPASGLLQSDYLPLYILIAIEVLAIAYMSIMLLQLSKKEQLEATGKAETWLSQL
                     WEKWNYKVPIEREDEMLISDHDYDGIHELDNGMPPWLQYMFFFTIGFAIVYLWYYNIG
                     NGPTQIDEYNASVEKAEIEKAAYLAAAGANYDENTVILSTDASVLANGKSTFQQYCVP
                     CHGANAEGTTTAPNLTDDYWIHGGKINDLFKTVKYGVQGKAMASWQEVLDPKQIFEVT
                     NYIKSLHGSNPANAKAPEGALYVEGGEAAPADTSAVTPDSLTVQNPATTVMQ"
     gene            29487..30884
                     /gene="ccoG"
                     /locus_tag="PLM90_00545"
     CDS             29487..30884
                     /gene="ccoG"
                     /locus_tag="PLM90_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HMZ87879.1"
                     /translation="MKDDKSFRDIIYTADEEGHRKWVFAAKPSGRFYNWRTIISYIYL
                     IAFFALPFIKVHGEPLFLFNIPDRKFILFGVIFWPQDFYLFALGMLTFLVFIIFFTVL
                     YGRLFCGWICPQTVFMEMVFRKIEYLFEGTAEHQRKLAKMQWNTEKILRKGGKQVVFF
                     LFSFLVGNTFLAYIIGVDRLYEIITEPLKENVGGLAIMMVFSFAFFIVFAFLREQICT
                     FACPYGRLQGVLLDKFSIVVAYDYKRGEPRGHHKHGEEDKQLGDCIDCGLCVRVCPTG
                     IDIRNGTQLECVNCTACIDACDSIMDKINKPRGLVRYSSENNIVTGEKLKFTPRMKAY
                     SLVLLLLVSVLTTLLATRKDVTATVTRSSGQLYQKRDSVLVTNIYNIKVANKTHESFP
                     IHLKLENVDGKVEMIGNEMHINSEDLAEGKFFIVLSRDQIHTMKMDVQIGVYNDDKKI
                     STVKTTFFGPVSKKK"
     gene            30887..31318
                     /locus_tag="PLM90_00550"
     CDS             30887..31318
                     /locus_tag="PLM90_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMZ87880.1"
                     /translation="MNWGRSILLVYVGFVALIMVMVFKSFNNGFDLVSEDYYADELKY
                     QDRINATENALPFADSIDVAVGNESIDIHFPASLAAMQSGEAYFYKPSDAKQDLRSPI
                     KMNDSGVQQFAKSEFSAGVYRLKMTWTMQDKTYYVEKLIKL"
     gene            31315..32061
                     /locus_tag="PLM90_00555"
     CDS             31315..32061
                     /locus_tag="PLM90_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ87881.1"
                     /translation="MTALLITAFITGLFGSLHCAGMCGPIALSFNMQGKGNAVINALL
                     YNASRIFAYALIGMLFGSLGVTFHYVGLQQVAAISMGVMILIIALITWLGAASSVKLP
                     LIKEYTNFIQRKIAQAFKSSSPSRIVALGFFNGLLPCGLVYLAVAGAIASGNTFVGAA
                     YMIIFGLGTLPLMFIFSAFGNWLGFRQRLFIRKYLPVFSITIAILLILRGLNLGIPYV
                     SPQFTHDQHNPVPADQHCSPAAQQEAHILQ"
     gene            32103..33197
                     /locus_tag="PLM90_00560"
     CDS             32103..33197
                     /locus_tag="PLM90_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMZ87882.1"
                     /translation="MTVADITHYLETIAPLSLQESYDNAGLQTGHPLQDVNAILISLD
                     VTEAVVDEAIAQGCNMIVAHHPVVFTGLKRLTGKTYTERIIIKAVRNNIAIYAIHTNL
                     DNVETGVNAMIAEKLGLVHGRVLQPKPGLLRKLVTFAPEAHAGEVRQALFQAGAGHIG
                     NYDHCSFNVDGAGTFRADATANPFVGKRGVDHEEAETRIEVIYPQWAETAVLRALLKA
                     HPYEEVAYDLYALGNNHKGVGSGWIAELPQAMEESQFLAMVAGKMQAKGVRYTALLGR
                     DIRKVALCGGSGSFLIKDAIAAGADAFITADVKYHQFFDAEGRILLVDIGHFESEQYT
                     SDLLARAISEKFANFAVRLSNVNTNPINYL"
     gene            33201..33944
                     /locus_tag="PLM90_00565"
     CDS             33201..33944
                     /locus_tag="PLM90_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HMZ87883.1"
                     /translation="MAVAKEMSVKESMQALWKLQRIHSKIDDITTLKGELPMEVSDLE
                     DEIAGLETRMGKLENELKDIETEISNRKTTISNSKAHIKKYEQQQNAVKNSREFDALT
                     KEIELNKLDIELSEKKIREANFAKEAKSVTIEEAKVILDNKKKDLKKKKEELERIIGE
                     TEKEEAELAKKVEKAEKDLEPRLIQAYYRIRESYRNGLAVVSVARNSCGGCFSVVPPQ
                     RQAEIKQSKNIIICEHCGRILVDESVMEA"
     gene            33969..35447
                     /locus_tag="PLM90_00570"
     CDS             33969..35447
                     /locus_tag="PLM90_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87884.1"
                     /translation="MKQYIIVLFMAFFIPASAIAQKYDFNPRCVEAYTYIQELRLEKG
                     RALLDKEKKENPSNLIPYLIENYIDFLTTYINEDPDQFKSLEKNKSTRLDKLKTGDQT
                     SPYYLYCQAEVTIQWAFSKLKFEEYVGAFTDVRKAYLLLEENQKKFPDFIANNKSLGM
                     LHAIVGAIPDQYKWGINMLGMDGSIDQGLRELESILEYSETNTFVFKQEVYLYYAFIA
                     LYLGHDAQTAWDIVKDLDTKTNLINVFCKASVAMRTGRNDLALQTLQSRPSGSEYLPY
                     HYLDFMVGLAKARKLDSDGAQYFERFLKNYKGKNYIKEAYQKLGWLALIRGDKDKYWE
                     YQYYVKSRGDDLIDDDKQALYEAESGHVPNVTLLKSRLLFDGGYYKEALKLLEGKSVD
                     SFSDKKDKVEFTYRAGRIYDMAKQPDKAKGFYLATIKNGETLPYYYAAASAYYLGIIF
                     ETQKDYPNARKYYQKCVDSDNKEYQTSLDGLAKAGLNRVKGK"
     gene            complement(35517..37016)
                     /locus_tag="PLM90_00575"
     CDS             complement(35517..37016)
                     /locus_tag="PLM90_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87885.1"
                     /translation="MLKNQVRLAIILITGIISMVSTNAFSQNYRLVDKDREMTFARLS
                     VTGKDSLFYFIKTDSVALEGLDSVYYFSTQFQNVAGDPDCEWVRTDTSILGFKMLIKP
                     DAQATHVFFNQHGDSIFIKSVVNIGDYWTVYKWPDGSFIKATVVNKLERTILPDVEDT
                     ILRIQFTVFNALNVIMPDSFPTETKMDISKFHGLTEFFNFNIFPLPGDSIGRVLRGLT
                     NPYENIVDVDAYRAYNFSLGYEFHYRETSVPDNQSDADKRISAIKYFIQTKTPLPDGV
                     SYQVERVQYDTLYNDGVPSVNLKWDTIELTFNYADFAYLDAHEFTLFDHIHAGYSDWV
                     KDTSLYEGVPHKNVYDHYVYNDATYCLSNPDNISQPVEIVGDGLGQIYYLDSLSADAY
                     YELSMVYFQVGLITWGTPYDFSSLDVAINQLAVEHLYVYPNPTLNYLHIGIPVQDDTE
                     MFIFDIQGRLVQKPVMHQGLINVSNLSPGMYTLAVYSENSFKSATFIKE"
     gene            37120..38139
                     /gene="murB"
                     /locus_tag="PLM90_00580"
     CDS             37120..38139
                     /gene="murB"
                     /locus_tag="PLM90_00580"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMZ87886.1"
                     /translation="MMVQIQHNVSLKPFNTFGIEVQTSAFTSIKTVSDLRELIHAGTL
                     QSSHMFLGGGSNVLFTKDFNGLVIKNECKGIYITKEDNRHIWITAGSGEVWHDLVMFT
                     VTRGLGGIENMSLIPGTVGAAPMQNIGAYGVELTNVFEQLTAIDLLTGEEVLFNHEMC
                     AFGYRESVFKHQLKGKYFIADVTLRLSKYPELQINYGAIKQVLAERGIVEPTIKDISD
                     AVIYIRQSKLPNPVVLGNAGSFFKNPEIAVEQYDELKQLYPTIPGYPTTPNKIKVPAG
                     WLIEQCGWKGKIVGHTGAHKDQALVLVNYGGASGNEILALSKEIQQSILAKFNIAISP
                     EVNII"
     gene            complement(38162..39640)
                     /locus_tag="PLM90_00585"
     CDS             complement(38162..39640)
                     /locus_tag="PLM90_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87887.1"
                     /translation="MKTRLYTILLSIIVTTNSGKAQNWDLFPYGAKLSYLERSTTRFS
                     VLCNDSINFDGLNTIYFCNAKAPKSSWQHCYDLLNASIPYKGNKIYFTGNGNEYLIYN
                     NIDSTNGFYFNTDIGLGDFWAVPIVNPASLYDELTVTFDSIDYINVLGDFDSVRYYSI
                     GVSPEPEEGTIFEGNVFTLSKNHGLLSFPNFGRLVNGVFCPDCLLMKDLVGFIQTDDT
                     LGLTIPKWSDYIQLQPDDILVYSSSTIISSHGVNDVDYFVDKITSVDKYPDSIVVNFI
                     RNDIYPGRNLFSRSVVQALFEGYNSSYHYGAFEGNHPTLTDGLEDGFYYISSSDGYNI
                     NSTRFENATCIEYSLTQAYVDTVDCTRHAYSPTTYSISSGIGLISSYANIYGWVYTQT
                     LVGSILSGHASGEIFASQFGASNKAVIEIQLLPNPTSDVIILPIACNENESYQVYNQY
                     GSLVGRGPADNPVINISDLASGMYVVFVKHDNLNYAGKFLKL"
     gene            complement(39842..40732)
                     /locus_tag="PLM90_00590"
     CDS             complement(39842..40732)
                     /locus_tag="PLM90_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ87888.1"
                     /translation="MYNQPMLPAQTFTSKVIIVTGGGTGLGKSMTRYFLELGATVIIT
                     SRKIEVLSKTAEELSAATGSTCIPIVCDVRDYDAVEHMLQSAIEKCGKVDMLVNNAAG
                     NFISPTERLSHRAFDSVVDIVLKGTYNCTLACGKYWIENKLSASVLNIVTTYSWTGSG
                     YVVPSACGKAGVLALTRSLAVEWGKYHIRLNAIAPGPFPTEGAWSRLVPPGMEDDFDM
                     AKKNAVGRLGEHQELANLAAYLLSDFAAFMTGEVVVIDGAEWLKGAGEFNMLDKLPTQ
                     LWDMMELMRKERGERREERE"
     gene            40785..41183
                     /locus_tag="PLM90_00595"
     CDS             40785..41183
                     /locus_tag="PLM90_00595"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ87889.1"
                     /translation="MATFSHQIKVRGFHCDMFQHVNNARYLEFLEETRWEWLNRMTSF
                     EFFEKHQMSFVVVSITIHYRWPSLLNDVLNITVETNHIGNRSASVKQVITRDGDSKLI
                     AEAEVTFAMIDNRTGKSVPISDELKAFFEN"
     gene            complement(41203..42492)
                     /locus_tag="PLM90_00600"
     CDS             complement(41203..42492)
                     /locus_tag="PLM90_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ87890.1"
                     /translation="MGNGITSTQRTLKVSHMADNLIGSEIIKLAGEVNDRIKKGEKIY
                     NLTIGDFDPKIFPIPAELEKGIIAAYQNNETNYPAANGIDILRKAAQYFVEKHLGLSY
                     SPDEYLIAGGGRPLIYATYITLLDAGDKVIYPVPSWNNNHYTHLSNCVKVEVATRPEN
                     NFMPTAEEIAPHVQDATLIALCSPLNPTGTAFSKQALEEICDLVLAENARRPADAKPL
                     YILYDQLYWTLTFGDTEHFDPVSLRPELRDYVIYIDGISKAFSATGVRVGWSFGPKAI
                     IDKMRSILSHIGAWAPKAEQVASAHFLTNDAAVDSFLMQFKSQVQQRLDGFYHHFTAL
                     KNKGYAVDCISPQSAIYLTVRCDLRGKRKADGTVIQNMADVASFLLNEAKIALVPFYA
                     FGSEKESPWFRLSVGTCSLQDVDDSMASLEKALSQLQ"
     gene            complement(42636..43685)
                     /gene="selD"
                     /locus_tag="PLM90_00605"
     CDS             complement(42636..43685)
                     /gene="selD"
                     /locus_tag="PLM90_00605"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HMZ87891.1"
                     /translation="MSTSLPPIKLTQYSHGAGCGCKISPKILDEILQSVGKQQVFPGL
                     IVGNETKDDAAVYDLGNGTGLVSTTDFFMPIVDDPIKFGKIASVNAISDVYAMGGTPL
                     MAIAILGWPIDKVDPRYAGLVLDGARQACAEAGIPLAGGHSIDCPEPVFGLAVSGLVD
                     LKQLKTNAGASEGCSLFLTKKLGVGILTTAQKKDLLLPEHADIAPDSMMQLNKPGAAF
                     GKLEYIEAMTDVTGFGLLGHLTEMCEGSSVSAEIVFDKVPTIDRSILDHYLSQKSIPG
                     GTNRNFASYGHKIAPLTDTQKAILCDPQTSGGLLVAVTAGFESAFMSYAATLGLQLTS
                     FGKLVAPKEHLVYVS"
     gene            complement(43698..44744)
                     /gene="mnmH"
                     /locus_tag="PLM90_00610"
     CDS             complement(43698..44744)
                     /gene="mnmH"
                     /locus_tag="PLM90_00610"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HMZ87892.1"
                     /translation="MTISAYLSDAGSLPLVDVRSPGEFATGHIPGAVNIPLFDNSERA
                     QVGILYKHEGRQAAIRKGLSITGPKLDKFIHEASALAKDNAIAVHCWRGGMRSQSMAT
                     LFDFAGFQTKVLKGGYKSYRGAVHALFSSQLTVYILGGRTGSGKTKILQALRNAGEQV
                     ADLEHLAHHKGSAFGDLGEEPQPSTEMFENLLFESIRNFDLSKPIWIEDESHLIGSVF
                     IPEALWQQMRNAPVFFCDVPIHERIQFLIHAYGQFPAEQLIGAVKKITKRLGGQHAKA
                     AVECFERGDLFKATEIVLGYYDKAYAYGLTQRAHQEIHNIEMSTINPEINAQQLIDHV
                     CRNAITEKNISTKS"
     gene            44792..46039
                     /locus_tag="PLM90_00615"
     CDS             44792..46039
                     /locus_tag="PLM90_00615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ87893.1"
                     /translation="MLNAIGKSFIALLLIASLHAQAQMPISTETYHATLDMQGQQLGF
                     QLELEITANSSEAYAPLNFTLVNGVEQIAGEVIKRNDSFVCIMPVFNTEIVFVGDNHL
                     QEIHGRWYDHSRQGNYFLPFKATQISQDEKRYRYFKQLTAPKANISGTFAVAFNDGES
                     TDNTVGIFEQKNNYLTGTFLTTTGDYRFLEGEVSGDKFYLSAFDGSHAFIFQGKIISN
                     DSIIGVFYSGIHYLSLFNGKRDANAKLPDESKLTFLKPGYDKISFSFPDENGKMVSLD
                     DAKFSNKAVIVTITGSWCPNCMDESSYLSEVEDKYKKQGLEIIALSFERKTDPETFAW
                     SINKVRTHFGTDFTYLNAGLPKTAGDALPMLNKVMGFPTMILLDRNHQVVAIHTGFSG
                     PATGKLFLDFQTKFEEELSRALK"
     gene            46199..47671
                     /locus_tag="PLM90_00620"
     CDS             46199..47671
                     /locus_tag="PLM90_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ87894.1"
                     /translation="MKYALYLMLLISTFSATAQVEITNISFEHNSADLSSDDLSKISE
                     IAGRLNASHDNVISVTMAGFTDTTGTVEYNEQLSWQRTKNVEKALHDLWPGEVTLEWY
                     GERNEGNAHAQTRSVDIIVSIETLPQVEIPKSNAENFIEVFSSVQQKAQQFYIPSNDS
                     SIIIGDQGTVIDIPANSFDIPTAYAGANVTITLKEYYLLSDMLLAGFTTQCGDDLLQS
                     GGMIDVNAYVAGKKVGLRDGKKLHIKFNTKRKLGEMELFAGERNEKGLVVWKRTKTPM
                     TATNTYEFISLLAPAYFKEVSDLKSCPYFFCGLRQQFGMNTTNSQNRRTYGKIDWPAY
                     RAYISEMKKSFGVKYMTELRRAIDEHNKKVGEINQYVVETSSLGLMNCDRFMNYPVEQ
                     RTKVIAESRQIDGASGFLAFTAYPAMLSVNYFEDGNIGFSNIGPDMNATLVLIRKDGE
                     KWMLSIQNIVTARDTRVTPAFVEVTPEELKTKLKMLDENG"
     gene            47871..48536
                     /locus_tag="PLM90_00625"
     CDS             47871..48536
                     /locus_tag="PLM90_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ87895.1"
                     /translation="MKHLFAITALFFTSYCYTQTSTTSYYGDVYLSTKAPQSKAKFSK
                     TTTLAADSTITTVVTDLKNNRIVSSESMKNNEAVGIWLKRHGGQTDTLDYQFDLIYAA
                     STCRESLPGLKNVWADNDSLHYKAPVLKDNESVYAAIGNEVYYPQFAKENAIEGKVFL
                     EFTITETGKIENIIVVRGVHISMDKEAVRTVKTLKFESLPMVNGVVTPLYVKMPLMFT
                     LAD"
     gene            48554..49414
                     /locus_tag="PLM90_00630"
     CDS             48554..49414
                     /locus_tag="PLM90_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87896.1"
                     /translation="MKTILLLLGVSIGATLFAQKNPTELAQPIVAEGKLLYKSEMASW
                     FGTDIFLEKFSDMNIVGGYFSYTEGDTSTCIFFSDAEKPEVLGEIQFDTTFNPSTAKV
                     DLERRSFTNLENNLFVIRSKALDELRSDTMFIRYNNTDFNLIPVIKDGERKVYVLTGT
                     ASNGVVLFGNDYLLTFDAENNLLTKKRIHQNLIPVYLNGPDAKNTVGTIHSHLPETGD
                     FMTATDICTAMLYQDITHWETITVVSEKYMNIWNCRTNTLTVVEKDIFKKIDEDQEKR
                     HGNKEKNNDD"
     gene            49525..50193
                     /locus_tag="PLM90_00635"
     CDS             49525..50193
                     /locus_tag="PLM90_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HMZ87897.1"
                     /translation="MLKKLVVTKRIKKIFFRTFILGSVSVILLILFCNFRIEKAGKGK
                     LYSDVSAIPHQHVGLLLGTGKYMSDGSVNLFYKYRIEAALSLLKSNKIDYLVISGDNS
                     RPDYNEPAMMRDDLVAAGIDISRIYLDYAGFRTFDSVVRLREIFGTDSATIISQEFHN
                     ERALYIAGKENITAIGFNAQDVGGSGGFKTQLREYLARVKVFVDYIIHTKPHFLGPKV
                     QIPQ"
     gene            complement(50204..>51526)
                     /locus_tag="PLM90_00640"
     CDS             complement(50204..>51526)
                     /locus_tag="PLM90_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ87898.1"
                     /translation="PALPHDEISQEQYAAMRSDVQHNIEVLSAQGKLVPAQQKTEALA
                     LEFPLAWNAGFSGYNFYGISNFADHNTAFPGALLDWNCGARTYDTDAGYNHQGIDYFL
                     WPFSWKMMQEEQVQIVAAAPGMIVARYDGNFDQNCAFNPGSWNAVFVRHDDGSTAWYG
                     HMKNGSVTAKGLGDFVETGEYLGLVGSSGNSTGPHLHFELYDADGALMDPNSGACNEW
                     NDESWWADQKPYIDPEINKLQTHFAPPTFPPCPEVEITNESNTFNVGDVCYFALYAKD
                     LSSTDLCHLKITRADGSVWYEWDFYQPADYFSASYWYWFYTIPEDVPEGVWTWSAELA
                     GKYYEHQFIVGDMPVDVHENGSYTSVNAYFQNNSLQLQLSSSIEQQSQIAIIDAMGRM
                     VAEKSTILQMGDNLITIPCETLSSGIYFIRILNSTTGATTTIPVCKQF"
BASE COUNT        15217 a        10646 c        10576 g        14987 t
ORIGIN      
        1 gcaaatctgc caatcggcca atcggctaat tggctaatcg gctaattggc taattcccaa
       61 taccccttat ctttgcacaa aatttcacca atgacaattt tgatcaccgg cgcctgtggc
      121 atggtgggat cgcatatgat cgacatgtat tatgcccagg gaatcaccga tctagtgggc
      181 acattttata agcctaccac cgatatggaa gacatccacg gcaaggctcg attggtggaa
      241 tgtgatgtgc gctactacca acgcgtgcac gatattataa ctggaaccaa acccgataaa
      301 attttccacc tcgcagcaca gagctatcct accgttagct ggcatcgccc gcaggagact
      361 atggatacca atgtcaccgg caccatcaat gttttcgaag ccataaaggc cgtgcgaaaa
      421 acagatccgg attatgatcc tgtagttgta gtagcatgtt caagtgcaga atacggtgca
      481 tcactgacac cggaaaatac ccctgtgaaa gaagaagctg cattattgcc tttacatcct
      541 tatggtgtga gcaaagtagg tcaagacctg ctttcatttc aatattggca aaatgacaag
      601 atacgttgca tacgcgcgcg aatttttaat acaacaggtc cacgcaaaac aaatgatgta
      661 gtggccgatt tcacaagtcg cgctgttgcc attgagcgta aaaaagcaaa tacacttaca
      721 gtaggcaatc tcaatacgcg tcgtgccatc accgatgtgc gcgacctcgt agcggcattg
      781 atgctattaa gcgataaagg aaattggggc gatgtatata atatttcagg aaataaagtc
      841 tatacgatca gcgaaataat tcctatcatc gaaaattata tgggtatgtc gctgacttac
      901 gaagtagatc cgaaattatt aagaccaacc gatgagccta tcatttatgg cgatagcaca
      961 aaattgaaaa acgataccgg atgggaacag aaatatgatc tcgctaccac catcagagac
     1021 atgatcgact atctgcgcag caagccataa tcatgatgtt gaatcgcacc cgtttatttg
     1081 acctcgatct gatcaatgat cacaacttcg atgcagtgat ttcatctatg atggattttt
     1141 cgcatgaata cgatccgttg tcagataaat tgccgctgct gttcacaccg aatgtcgacg
     1201 atgtagtaaa actcaacgaa aaacaatatg ccgatctcgc atcagtgttg cgcagaagta
     1261 tgtattgctt gccagacgga cagccaatta tctgggtaag taaactattc aaagggcgaa
     1321 aactgaaaag gcgtttgccg ggaagtgaat tgtttccttt gttatggaag gaagtcatca
     1381 gcaaacaaaa acgcgtgatg ctcgtagcgc catcggaaca agtaggggag ttgttgaaaa
     1441 aagaatatcc cgcaatggta tattatgtgc cgccattctt cgatgtgcaa gatgaagtac
     1501 aattgaaaaa agtgcaacag gaagcagcag caatttacga tagcattatg cccgaattag
     1561 tgttcatcgg cattcgctac cccaagcaaa accatatcgc actcggactc atcgatcatc
     1621 acaatcgaac ctatgccaca ccacgtggaa aggaagaaaa agtaggcatc aggtcctatc
     1681 aactttatgg cagcaacagc gcacaaccgc atgccttgca tttattaatg ggcgccagct
     1741 acgaattcta cctcggactc aaaaaccgtg caccacaatt ctggcagaaa atcggcatgg
     1801 aatggttcta ccgcttcacc caggaacccg gcagattatt caaacgctac ttcatcgacg
     1861 atctccaatt tttccccata gtgatgaaag aatttttcaa gtcgaaataa ttttttaccg
     1921 cgattataat atttaatttt tgggcgtatc cctgcgggcc gggctatccg ctgcaagtcc
     1981 tcattccgct tcgctgcatt ccgggctttc cgctgctatc ccttacgcgg ataaatttgt
     2041 tcatgtttga ttttgatttt tgcagtacat gaatcgtatc atcaaaattt ttttattaaa
     2101 catgtgaccc cttcggggtc ggggagatgg caatgtgatt gattaattga aatgatgacc
     2161 cctacggggt cgtttttgct gcggggtacg cacacccgac cccgaagggg tcgcatgttt
     2221 gtatcccccc gcgccgcgcc gccacaccgc acactttacc ccgaaggggt cgcatgtttg
     2281 tatcccccgc gccgccacac cacacccgac cccgaagggg tcgcatgttt gtatcccccc
     2341 gcgccgcgcc gccacaccgc acacccgacc ccgaaggggt cgcatgtttg tatcccccgc
     2401 gccgccacac cgcacacccg accccggagg ggtcgcatgt ttgtatcccc cgcgccgcgc
     2461 cgccacaccg tactcgaccc cgaaggggtc gcatgtttat ttcgggattt attttgttga
     2521 cagcacaaca ccatcagaaa taaaatctac ctgtttggtg atatcccttt cagctttctg
     2581 tatctatcca ctcaaataaa tactcatctt tatatgcaat ctcgaactct tttaagtagg
     2641 cataatactc ctctttaaac gattttactt tgtgatgctc cttttggttc atgatgtact
     2701 ttataacgtt gtcaatactc gagtggctat atgaaaaagc accaaaccct tcctgccagc
     2761 tgaacttcga tttcgtgaaa ttgttttctt tgataaactc agttgaagcc tttttcacct
     2821 ctcttaccaa atccgataaa caacaagacg gccgcatgcc aataaatatg tgaatatgat
     2881 ccgacatccc atttattgct aataacttct gctgcttgtt ggtgaggatg ccggttatat
     2941 atttatacaa ctcctcctcc cacgaatatc gtatcagaca ttctcgatgc ttcacagcaa
     3001 aaacaatctg tatataaatt tgcgaatagg tactcgacat gtagaactca atttttaagg
     3061 gaatgacact tttttctgcc tgcatccact aaatcaatat catcatgttg ataattcagc
     3121 tctgccggat aaaaatatca tttaataggc tcaaacctat acaaatttca tgcactttcc
     3181 cttaaattag aaattgaatt ttttcgatga ataagaagta aataactcat atcacggagg
     3241 aaattttctg catccggtaa aatttcagaa tgtaagcggt tagcagttat ttaatcgata
     3301 ataaaatatt tacaattcga ttatacaata taagtcatta tcactgcaca ataaatccct
     3361 tcgcaccaaa atcactcttg gcaatgtaaa atccgttcgc ccaggaatgt gtatctatgt
     3421 atacgtgctg ttgcgagttg atctgataaa cgagctgccc gattgcattg aagactgaga
     3481 tatcacttga ttcgggaagt aatatattta catagctgtc tgcaggattg gggtaaagaa
     3541 gcaagtctaa tttcccgtca gtagttggaa atgatgtggg aacgcccatc gaataaaata
     3601 atccttcata tccggcaata aatccgatat cagcacttaa aagctgcaat tcatgcaagt
     3661 cgcccggaat gtcgtaaata gtgtcaaggg gtatgctaac ccatgtcctc cctgagtctg
     3721 tggttttctt tagcaaatag tcatatccaa caaaataacc gatagaatcc tggaaagcca
     3781 tgtcattgat ggcaaaaaca tcagcatcca taacaccaag tgtgtcccag gttgcacaac
     3841 tatctttagt gcgaaatagt cgaaatccga aatcagcatt tttagttgcc atcattattt
     3901 ggtgattggg atatatgaat agcagtttga tcgcatcttc aaccgatgta taaattaaat
     3961 cccatgtgtc accggcgttt gaactgcgaa aaatgaagtc atttgtatag gcatagcaag
     4021 tagaccctat gcactgcatg ccgataaatg attgataatg cggttgaaga aataatacgc
     4081 gggtgatatt ttctccatta tctgtgatct taaatatttc acctgaatac cctgcaatca
     4141 atacagtact atcatcaggc atttgtaatc cattatataa aaaattagta ccccccagtt
     4201 caatcaattc ccaatctttt cctccgtttt ctgtccgata aaaattgcta tatcgatttg
     4261 atgtataacc aacatcacca acaaaaacca cgttgaaagg atagttttca tcgcattccg
     4321 aatcaaacaa agtatcccag cttattccgc catcaaatgt ttccatgatc atccctccat
     4381 cccaactatc taatagggtg gaatcaccgc cactataata accatgcgta ggatcggaaa
     4441 accaaatttg tgaacaacat tgcggacctt ctacgggtaa caaggtccat tgggcatgaa
     4501 tgggctggca aaaagccgca aacaggataa atagtatgta tttcatgggt gggattactg
     4561 ctttcaaaga aataattttt tgatatttat tgattttctg atagaaaatc ggtgctgttg
     4621 tttaatctaa tgtatttcac cctcataatt ttcattaccc ctatttgcca ataattcccg
     4681 acctttgcac caaatttgga agtaaggttt aggcatcatg caaccaaata tcagtattat
     4741 tcggtctacc ggctacctcc cgcccccaac tcgggacccc gcaaaaccaa ataccaaata
     4801 cctgatactt gatacctgat acttgatacc tgatacctga tacctgatac ttgatacctg
     4861 atacctgata cttgatacct gataccagat aaaccaagag ataacacaaa aatgatcatc
     4921 agaagcaaag cccctctccg catcggactc gccggtggtg gaacagatgt aagcccttac
     4981 agcgacattt acggcggatg tatcctcaat gccactataa ataagtatgc ctacgctacc
     5041 atcctgccac gcaacaatgg caagatcgta ttgcatgcca tggaccgcaa tgaacgcatc
     5101 gaactggata gcatatccaa gctacctatt gatggcaccc tcgacttgct gaaaggcgtt
     5161 tataaccgca tcgtaaaaga ttttaaccac aacgctccac tttcatttga actcaatact
     5221 tacgtggatg cgcctccggg atccggcctc ggttcttcat cgacactcgt tgttgcaata
     5281 gtaggcgctt tcaccgaatg gttgaaattg ccacttggag agtacgacat cgctcacctc
     5341 gcttacgaca tcgagcgaaa agaccttacc atggccggtg gcaagcaaga tcaatatgcc
     5401 gctactttcg gaggatggaa tttcatggag ttttataagg atgacaaagt aatcgttaac
     5461 ccgctccgta ttcgtcctca atacctccag gaacttgaat tcaatatcgt gatgtactat
     5521 accggcacaa gccgtttgtc gtctactatc attgaagcgc aaagcagcaa cgtgactaaa
     5581 aaatctgata agtcgatcga ggctatgcac aatctgaagg agcaggcata tatgatgaag
     5641 gaggcgatct taaaaggtga actcaatcgc atcggcgaca ttcttgatta cggctggaaa
     5701 aataaaaaag ctacggcatc aggtgttacc agcgatgtga tcgacagtat ttatgaaagt
     5761 gctatcaaag caggcgcaac aggaggaaaa atctccggcg caggtggtgg tggtttcatg
     5821 acattctact gccccggcaa tactcgatat aaagttattg aagtactgaa cacctttggt
     5881 ggcgaagtac ataatctcga attttcaaaa tacggactga catcatggga agccaaatag
     5941 aaaaaattaa aagcgtgatc gctgagagca tccgcgttaa aaatgacatc ttgaataatc
     6001 ctgaaatgat cgctaaatgc gaagagatcg ccgaacgcat tgccgaggct tttcgtcagg
     6061 ataaaaaagt attattttgt ggcaatggtg gctctgcagc cgacgctcaa cacttggctg
     6121 ccgaattttc aggtcgcttt tatttcgatc gcccaccact ggacgccgaa gcattgcacg
     6181 tgaattcttc ttatgttact gctgttggaa acgactatgg ttatgatcag gttttcagcc
     6241 gggtattaaa agcaaaaggc cgccaaggtg attttctttt cggcatttcc acttccggaa
     6301 attcaggtaa tatcctgaaa gcttttgaag tagcacgcga acgcggaatg gtgatcgttg
     6361 ccatgacagg tgaaagcggt ggtaaaatga aagataattg cgattatctg ctcaacgtgc
     6421 catcaaatga tacaccacgc attcaggaaa gtcacattat gctcgggcat attatttgcg
     6481 aactcgttga agcgtcattg ttcgaacgtc catgatcagt gaagcaatag tgcttgcagg
     6541 tggctttggc actcgcctac aaaaagtagt tgctgaagtg ccgaaaccta tggcgcctgt
     6601 agccggaaaa ccttttctgc actatatttt cgactatctc attaaaaacg acatcaccca
     6661 cgctgtattg gcggtcggat atttgaggga agttattatc gatacctatg gcgataatta
     6721 tcgctcatta aaacttacct attccatcga aacagatccg cttggaacag gtggtggcat
     6781 catgcaggca tgcaaatatt tgcaaggcaa cgaagcaatg gtaattaatg gcgatacttt
     6841 ttttgatgtc gaccttcacg cactcacatc gtttcatcag cagcaaaaag ctttgttgag
     6901 tgttgcgcta aagcggatgg aaaatttcga cagatacgga accgttgaaa caaacgatac
     6961 gcatcaaatt acgggatttc gcgaaaagag atatatgtct gagggactta ttaatggtgg
     7021 gatttattgc ctgaatactg caatttttcc tgagcatatt cctcagaaat tctcctttga
     7081 aaaagaaatt cttgaaaagg aaatacatgc cggtagaatt ttcggaatgg agtcgcaagg
     7141 ttattttatt gatataggaa tacccgaaga ttatgcccgt gcacaggatg attttaaatc
     7201 gcgctgacca cttttaatcg ccaaattcat tttttaaggt gcaaaaaccg gttccacaaa
     7261 tcgatcaatc atggacgcta ttcctcgaca gagatggcgt attaaatcga aaaatcgatc
     7321 acgactatgt gcgcaattgg tcgcagttcg attggctccc cggtgtaaag gaagcactcg
     7381 cttatttcgc cactcgtttc ggtaaaattg taattgtcac caatcaacgt ggggttggtc
     7441 gcgggtggat gacacttgaa gacctgaatg acatccatac acgcctcaaa caagcggtta
     7501 cagatgcagg cggtcgaatt gacggcatct atttctgcac cgacacaacc gacgaagggt
     7561 caacacaccg taagccaaaa cccggcatgg ccatggatgc taagggcgat tttcctgcga
     7621 tagacttctc aaaatccatc attgccggtg attccggtag cgacatacaa ttcggcaaaa
     7681 acctgggcat gtacacggta ttgattgccc ccgaatctaa acatgatgca gatttaacag
     7741 tacccggact tgcagcattt gcaaggcttt tacagcaaaa tgattaacgg ttttccggta
     7801 ttttcactgt ttacgcataa aatcttacct ccttttacta tttttgcaat agcgcttggc
     7861 cctcaacaat cgctgcatga taacacacac gatgcaacct ttgagatgtg aaaagcatca
     7921 gaaaagggta tcggagaatg agcacaaatg gtaagaaggt attcacaatt tctcgagtta
     7981 gtttacttcc tggtagatat tgttctgctc aatgtctcct ttcttatcgc cattttcatc
     8041 aatttcgacc gtatttctaa ggttagcgac gaccgcaagt ttgctatgct ccttgttgct
     8101 gttaacctca tttggttcat ggttactacc gttaccaatt actacaaatc tgagaaggaa
     8161 cgccgaacag gctatgaaca tcttgttttc aggtttctca aggtgctggc atttcaggtc
     8221 atgttaacgt ttacctatct gatcctcatc aagggatata ctttttcacg tttacagctt
     8281 ggatatacct acatattttt tactgttctg gatcttggat ggcgtattac cttcgaaact
     8341 tacctgaaga gatatcgggc acgcggaggg aactatcgcc gtatcatcgt tatcggaact
     8401 acccaggcct cgcttaattt cgtaaaggaa attcaggaac acaatgaatt tggatatcgc
     8461 tttttcggct attttgacga tcctgaaaaa tcgatggtgg aagtggaagg tagtttggaa
     8521 gatgtgcaaa gctattgtcg caagcataat attgacgaag tgtacttcgc gctccatcct
     8581 tctgacatga gtgaatatgt agatgacttg ttgaattttt gtaacgataa tctcgtgcgc
     8641 ttccgcatgg tgcctgagtt ctcctcatac atcagtaaac aattcaaaaa agtaaatatc
     8701 gaatattacg gcagtactcc aattttgagt attcgtcctg aaccattgga taatttatac
     8761 aaccgtttgc tgaaacgttt attcgatatt gcctttgcat tattgttttt cattatcatc
     8821 ggatggtggt tattccctat cattgcacta tttgtaaaat tatcttctac cggaccggta
     8881 ttttttattc agaaaagaag tggtgaaggg aatgaagaat tttggtgtta taagtttcgc
     8941 accatgaagg tgaatgccga agccgatacc aaacaagcta ctaaaggaga tccgcgtatc
     9001 acaagagtgg gcgcttttct gcgtaaaacc aacctcgatg agttgccgca attcataaat
     9061 gtattacttg gcgatatgag tgtggtgggc cctcgtccac atatgttgaa acatacggaa
     9121 gaatattcca aaatcgtgaa taactttatg gtgcgccatt tcgtgaagcc gggtattacc
     9181 ggtgccgcac aagccaatgg ttaccggggc gatactaccg atccggtaat gatggaaaaa
     9241 cgcgtgcaat acgacctttg gtacctcgag aactggagtt tctggctcga tgtcaaaatc
     9301 gtattcctca cagtatggag catgcttaaa ggaaacgaaa atgcgtttta gattactcac
     9361 cctcctcatc gcgatcagca cagctgcttc cgcacaacaa acttttttac cgctcaaccg
     9421 aaacatttac gcatggtatg aacctgctgt aaatgaacaa ggtgttctgt ttcatactgc
     9481 cattaaaccc tggcgtatgc aagaattgca acaggttgtt acctatgact cggtatttca
     9541 tttgaaaaaa gtaagcgatg ataaattcat cggaaaagtg tggaacttct ttggctatga
     9601 tactattctt agagtgaata ccgaagactt ccacttgcgt gtcgaccccc tgatcaattt
     9661 cgccggaggt atggatattg agaatggagg aaagggttta tggacgaata cgcgtggtat
     9721 ttccgtgatg ggagatatcg gtccggaacg caaagtttct tttcaggcaa cacttcgcga
     9781 aaatcaagca aaatatcccg actatctcgt tccatggatt aagtattaca acgttgttcc
     9841 cggacaagga aaagtgcgtt ctttcaaaaa cagtgctttc gattatgcaa atgctacggg
     9901 atatgtatcc tatacgccga gtaaatattt caatttcgaa ttagggcatg ataagaattt
     9961 tattggcgat ggttatcgca gtatgctatt aagcgataat gccctttatt accctttctt
    10021 caagattaca actactgtgt ggaagatcaa atacatgaac ctttacactg agtttgatga
    10081 tgcctataat acgctggacg attcagtaat ggttgtaacc ggtcttacgc gcaaattcgg
    10141 atcttttcat tatttgagct gggatgtttt cgattgggta caagtgggtt tatttgaagg
    10201 tattgtttgg cctgcgaatg attcgactcg ctatcgcgga ttcgatgtca attatatcaa
    10261 tccgattatt ttcttccgac ctattgaatt tggtttgggc tcacctgata atgcattaat
    10321 aggcgcaaat attaaattca aaccggtaaa taacgcaatc atttatggtc aatttttaat
    10381 ggatgactta gatgtggctg cagcaagggg aggagagggc tactatcgaa ataaatttgg
    10441 catacaaggt ggatttaaaa tctataatcc gtttaaaatt aacaatttga tgattcaagc
    10501 tgagatgaat caggcaaatc catacaccta tgcccacaag gaaccggtgc agaattatgc
    10561 ccactataac cagccacttg cccacccact cggagccaat ttccgggaat ttattggcat
    10621 tattaattat cgttttttcg aacgtttcta tatggaggcg aaggtccagt cggctgtaat
    10681 tggttacgat cagcatgccg gtgatcattt cggatccaac attttcattt cagactacga
    10741 catcccgggc ttccctgatt cctatggaaa ttacatcggc cagggactca aaactactat
    10801 ccgtagtgcc gaccttcgag gtggatttct gatcaatcca agtatcaatc taaacctcga
    10861 aggccagata tttattcgtc gcttcgccaa tgatcaggag aagattaata ccaccatgtt
    10921 catgctggcg cttaaaactg atttgttcaa ttcctactac gacctctaat aacgcccgtt
    10981 tttcagtacc tttgcacccc caaaagttct ttgattggtc ccgtagttca atggatagaa
    11041 tagaagtttc ctaaactttt gatacaggtt cgattcctgt cgggactacc taaaatcgat
    11101 taatggctcc gtagttcaat ggatagaatg aaagattccg gttctttaga tggcggttcg
    11161 actccgtccg gggctacaat tcagcaaatt attccggttg tttagaggag cagttccttg
    11221 cccgactgca catcgcggtg gagcaggcga gactccggaa gtgctgtaat caaccatttc
    11281 aaccaatatc tcttcaactt cagtcatcaa ttttaccctg catttctgct atcttcgcgc
    11341 cgtgtcccgc aggtatattg ccttaacgct tctggtagcc ttcacactaa aactgtgtgc
    11401 cgcctggtta atccatctct ggcaaataca aagtatacat gcctatcaac aggaaaaaat
    11461 cgaatccggc gatttcaatg gcgaaaccat agctcacctc gtggttgatc gtgaaatgat
    11521 ctcatgggtc gaagaaaatg aatttgtttt cgaaggatac ctctatgatg tgatcaattt
    11581 ggtcgagcac gatggctatc tttcaattgc tgcaattaaa gatcataagg aaactgaaaa
    11641 agatcataac tataaacaaa atcagaatca acagcaaaaa gaggaaggtg gtagaaatga
    11701 attgcagact actactattc cttttaccat tcctgcttat ttgcgcgtga tttttgaaca
    11761 aagtagtcga ttattaaccg cattgccggt cgataatatg caaactaact tcaccttaaa
    11821 agtacttgca cctccgcctc gagcataata tgttattaga gctttaatta ttttatttca
    11881 ctcaattaca tattatgaaa aaaattttat taatgagcgc aatgctctgt actatagctg
    11941 cattcgctca gcaaacagaa actacatacg atcttaacga agttatcgtt tcaggaaatc
    12001 gattcccgga agaacgtcgt gaggtagctc agaaagtcga cgttataaaa acatcgacaa
    12061 ttaatttcac aaatgctgca accactgctg aactactttc agaatcagga aaagtatttg
    12121 tgcagaaaag tcaactcggt ggtggcagcc cggtattgcg tgggttcgaa gccagccgtg
    12181 tattgatggt tgtggatggt gtgcgcatga acaatgctat ctatcgcgcc ggccaccttc
    12241 aaaatgtgat tacactcgat cagtcaattc ttgatagagt ggagattgct tatggccctt
    12301 catctcttgt atatggcagc gatgcactgg gtggtgttat gcatttccat acaaaaaatc
    12361 caaagctgct tgttgatggt gaaagcatgg cttcaggatc tgctttcctg cgttacgcaa
    12421 ctgccgcaga tgccatgtca ggtaatttca atattaatct cgcaggaaaa aaaatcggtt
    12481 cgcttacttc tgtatcatat aatgattata atgacttgcg ttcgggaaca gttgaaaatg
    12541 atgaaaacaa aaactattgg acgcgtccat actatgttgt tacgacaaac ggcgttgatg
    12601 agttgaaagc aaatgatgat aaatacacac aggtgtattc aggatatacg caatatgatg
    12661 tcatgcagaa gttcctgtat cagcaaagtg caaagctttc acacaaattg aatttccaat
    12721 actcaacatc taccgatatc ccgcgctatg atcgtttaac tgatccattg aatgcaacta
    12781 caggagatag tcttcgcttc gctgagtggt attatggtcc acaagatagg atgcttgttg
    12841 cctatgatat gttgtatgcc ggaaaaacag ctgctttcga caatatgcat gtgatactca
    12901 gctatcagga tattcaggaa agtcgccatc aacgtcgctg gaataaaaca aatctgcaac
    12961 atcgtatcga agatgttagt gtaatggggg caaacattga tttcgagaaa aaaatcaatg
    13021 caaacacaat cacttacgga ctagaatctt atttcaacga tgtaacatcg tctgcacatg
    13081 aagaagatat tcttacaggt gaagaaacac ctttagacac tcgttatgcc agcggtggcg
    13141 ctaccatgat gaatgctgct gcatttgctt cgcaccaatt gaaattcagc aacaataaat
    13201 ttgtattaaa tgatggcctg cgttttaatt attcaaaatt gacagcatcg tttactgata
    13261 aaacattctt cccattccct tatacagata tcgatcaaca aaatacttcg ttaacaggta
    13321 gcctcggttt agtgtattcg cctgataatt caacaaagat atccttgatc ggttctaccg
    13381 gtttccgcgt gccaaacgtt gatgacctta ccaaagtttt tgaaagcggt ccgggaagtc
    13441 ttatcgttcc aaatccagat cttggccctg aaaaaacaat taacgccgac ctttctattg
    13501 tgaaggtgtt taaagacaaa ctcgaaattg agcttaccgg tttttacacg cgatttaccg
    13561 attatatcgc attggaaccc ggaactttcg aaggagccga ttcaattgaa tatgatggcg
    13621 tgttgagcga agtacttaca acagtaaaca aaggcagtgc ttacctctat ggatttaatg
    13681 ttggtgccga tgtaaatatc atcaatggac tgaatttgtc atcatacatt acatacacat
    13741 atggtcgcat agaaaccgat accactccat atccactcga tcatattccg ccggtttacg
    13801 gaagaacagg cctcaaatac gctaaaaaga aacttgttgc cgaagcttac gccctgtata
    13861 acggatggaa gtatattgaa gattataaca tcgttggtgg tgaagataac gaccagtatg
    13921 ctacacctga aggaatgcct tcatggtata ccctcaatat caaagcatca tacgacgtaa
    13981 tggaatatgt taccatccag gcaggttgtg aaaacctcat ggatcaccaa tatagagtat
    14041 tcgcctccgg aatttctgct ccgggtcgca atatctttgt tgcggtgcgc gcgaaattct
    14101 gataaaaaga acccgcccct gtaagtctga cctcaggata ctgcccccgg agccctgatg
    14161 tcaacaccac aggggcgtgg tgttttcgtg aaaagtgata acttcacagc ctaaatatga
    14221 aacaatgagc ctcttttcaa aacttcttgg cggcaataaa ggcggtggcg gaggcaacaa
    14281 agcccaggct tttatcaccc aactgaccca ggacctgcat ctttcaagcg accaggttac
    14341 tcaggttgaa gcagcgttgc gtgaattctt ttccgaaaag cgggaaatga aacaatccgg
    14401 taacaaagac ggattcaaag aaagcaagca ggatttcaaa gatgatatcc tcaaagcttt
    14461 aagtcctgaa cagcaacaaa aatttatgga taatatccag acttataaac aattgcttag
    14521 aaagtaaatc ttttcaaggc aattgaaatc agtttttatt taaccacaaa ccacaccaca
    14581 tgacagattt actgttcgca aatgtattgt tgttcggttc taatgagcca acagtgcatc
    14641 ttcccgaagg attgtataag gccttattgg catcgcactc cggcttgcgt tatattgtaa
    14701 tgattttatt aatccttgca gttgtttacg ctcgtcaaac ttcatccgga aaaaaacctt
    14761 tcgccggtgc tactaagaaa atgggtatgt tcaccatgat cttagtggat attcaattat
    14821 tggtaggagc tgcactatat ttcaatttta tcgcatctca aacaaatttt aaattgggaa
    14881 aattgaaaga tcagctgagc gctccaatgt ttcgctcaat agcgctggat catgcgctgg
    14941 gaatgtttat cgcaatcgtg ctgattcact tagggtatgc taaagcgaaa aaatctatga
    15001 atgatgtaga tgcaggaaaa aaacaattta tgttttacct gattgcactt attctgatac
    15061 tcgtttctat tccatggcca ttcctgcatc atgaacgcgg atggttctga taactgaaaa
    15121 attgtacata aaaaacgggt ctgttacaac ggacccgttt tttatgcagt ttggtttctg
    15181 ttaatcgtac aattcacaaa cttttgccaa tcctttcgga tctataattt tgatagcatg
    15241 attgtttgtt tcaatcaatc cctcttcctt caaatctttt aatacacgaa ttgcagtttc
    15301 ttttaccgta ccaacataag acgcaaggtc ttcacgtgta atactcacaa cgccatccgg
    15361 attgtcctcg gttttataag attcacttaa tagtaacaac gcctccgcaa cgcgacctct
    15421 taccggttta taggcaatat cggccatttt ctcaacggtt tgatcaatgg tcttggtcag
    15481 cagttgcacc atgccttcag aaaagcgatt gttttcacgg aatatcttca acacggaatc
    15541 tttcggtata aagcatatca cggcatcttc aattacctcg gcactcacac cgtaagtttg
    15601 agagctcaac atcgccccat atccgataaa atcgcccggc gttacaattt tcatgatctg
    15661 ttccttgcca tccggagcct gtttatagag ttttaccttg ccctcattga gacaatacaa
    15721 cccatatgag ttggcgtgct cgtggaatat ggtctgccct tttttataca gggcacaggt
    15781 cttagcctga ctgatatatt ccgcttcgtc cacatgacaa aagtggaaca acgttttgtt
    15841 cctgctcttg cattgggcgc actcgggggt ttgaattcgt gatttcgtgg tcataatttt
    15901 tgaatttgcc tgaattgtca taaaattatc atttatcgca attgtatgca agtagtggta
    15961 tctgactgta taacgcaata ttgcgtgtta cactcatatg gaagatggta gaaaacagtc
    16021 cgtgattgtg tgtcaccatg cctatcatgt ctacctgatt cctcgaagca aacttcttga
    16081 tgccttccgt aatgttcttt tcagctaaaa aatgcaattg caatcgttcg gcatcaatct
    16141 ctttcctgaa accggtttgc agcttgtcta ccaatgcttt gttatatgaa ttggtatcat
    16201 tcatgaaatg aacagcatgc aacgtggcat tgtatttagc ggtaaatcct aacaaataac
    16261 caatgatatc ataatcctga tcggtgcctt cgaacggtac cataacgttt ttaatagaaa
    16321 ccggttcatt atgagtaggt aaggcgatca ccggaatgtc agatttcttg atgatattcc
    16381 atgtaacagt gccaaacatc ctgtcctcaa tactattggc cccctgggtt cctactacta
    16441 tcaagtctac tttttcggta tgggcaatgt ccaagatggt ttctgacgca aaccccatca
    16501 ttgccttgaa ttggacttta gtccacttta agtccgtacg atgcttaccg gcatagcgct
    16561 gcatatcatc atcggctctc aacttttgag cggcaagtaa ttcttttacc atatccggtg
    16621 gcatatgctc atcggtaaac ggcatgtgat aagcatgcac aactaataat tcggcatcaa
    16681 actgcttggc aatatcaaca gccaaaacca acgccaaatc ggcattttcg gagtagtcgg
    16741 ttggaaccaa aatgcgattt acctgcataa gtcgctcttt gaactgttca cgcttttgtg
    16801 ttcgcgttct gctcgattct tgcttgcccg gtccatttat cgaggacggc atgctgttaa
    16861 gttggtcgtc agtcattgga aagatttttt tactgattca aaattgaaca tagttcaccc
    16921 gaaacagcat gatatacctc aagccaaatg gtgacttttg tcaatccaaa aacaaaaaac
    16981 ctgcctgaaa agctgtttca ggcaggtttt ttttggggtt tgaagtagta tccggttgtc
    17041 tgtatccgga ttttgccttc ctaccgcaca atttcgatga tttattttac cattcttcct
    17101 cttttttagg agcggcaggg cgtaattcag aatcgagact ttcagtgagt ttattcataa
    17161 agtcctctat ctgaaccaga tagccggtaa cattctcatc ctttgcaaag ttttcttcgt
    17221 tgatccactt ttcaattccg aagtaggaag gaccttcaac atgtatcttg gttattgtgt
    17281 atttgaacct gccgtcgcga aactgcaatg tcagataata attgatctgc gcagctttta
    17341 cggtttgtcc tttttcgtcg cgcgtaagct gaaactgatg cttgatctca atcacgccat
    17401 cttctttgct ttggcttttg atagcctggc ttggactctt atagaatttc ttcatccatt
    17461 ccatagctac attgtacagt gagtcttttg taatgccggg aacttgaata acatcggtat
    17521 aagttaccag ttttgtttct tcgtcgacag gaaatgcggg cctttctact ttttcgcctt
    17581 tctgggcaaa tgcggccact gagcccatca ggcatacgat agtgaggaat ttcttcatga
    17641 aagtgctatt ttgcgacgaa gataatccca aaaacaatac cagattttca ccaatttgag
    17701 gttatgcaca gtttttgccc ctaatgattt aaaattttca tcaggatgta tacctttgtc
    17761 ctaaataact gcatcatgag ttctcagttt tttgtaggtc ttgttatcgt ttgctacatc
    17821 cttgttgttg gcggattgtt tgtgatagaa tatatcaaag gcaaacgcgg tggaaatacc
    17881 aacggccact aataatccat agccctcctg tccgatgcag aggataactc ctcctgcctc
    17941 cttttttgta cgatctgtaa tcttatttcc aagccggaaa tggccctctt tttgcaggcg
    18001 ataaaccggt cattttacta ccctcggtaa tgtgcattag gcgtgaataa attgcctttt
    18061 ttatgccttg attttactag gtaatcaggg gtaaaaatgg taattttgca gacgctcaaa
    18121 acaggggtgt ttttactcct gaaatgcaag tgaaaatcat ggaaaaagaa gcagaagaat
    18181 taaagaagaa taacggttac agtgccgata gtattcaggt gctcgaaggt ttggaagcgg
    18241 tgcggaaacg cccggccatg tatatcggtg ataccggttt caaaggactg catcacctcg
    18301 tttatgaagt agtagacaac tctatcgatg aagcccttgc aggttattgc aggaatatcg
    18361 aagtatctat ccttccggga aatagtatcc gtgtgaaaga cgacggtcgg ggtattccta
    18421 cagaattaca taaaaaagaa aatagatcgg ctctcgaagt ggttatgact gtgctgcatg
    18481 ccggtggtaa attcgataag gattcataca aggtatcagg tggtcttcac ggcgtgggtg
    18541 ttagctgcgt gaatgcatta tctactcacc ttaccgccga agtgcatcgc gaaggcaaaa
    18601 tatttaagca ggaatacgaa acgggtaagc cgctttatgc cgtaaaagaa gttggcgaat
    18661 caaatatcac aggaactatc atcaccttcc atcctgatga taccattttt acggtgtcag
    18721 aataccgata tgatacttta gctgcgcgtt tgcgtgaatt ggcatacttg aacaaaggaa
    18781 ttcgtctcat ccttaccgat gaacgcacag ccgatgaggc aggtaatttc gtcagagaag
    18841 aattttatag tgaaggtgga ttgattgagt tcgttcgctt tcttgatggc agcagaacgc
    18901 ctatcattcc cgaaccaatt tatgctgaag gtgcccgcga tggcgttgcc gttgaaatcg
    18961 ccctgcaata caacgattct ttcaacgaaa atgtacattc atacgtcaac aacatcaaca
    19021 ccattgaagg tggaacacat gttgccggtt tccgtcgcgc acttaccaga acattcaagg
    19081 cttatggcga taaggaagga atgttcgaaa aagctaaggt ggaaatcagt ggtgatgact
    19141 tcagagaagg ccttacatca ttgatttctg tgaaggtgcc tgagcctcaa ttcgaaggac
    19201 agacaaaaac aaagcttgga aataatgagg tgctcggaat tgtggatact atttcaggtg
    19261 agatgctgtt caactacctg gaagaaaatc ctaaacaagc acgccttatc atcggtaaag
    19321 taattcttgc tgctcaggca cgtatcgctg cccgcaaagc acgcgaactt gtgcagcgta
    19381 aaagtgcttt cggtggaggt ggtttgccgg gtaaactggc cgattgcaca gataaagatc
    19441 cacaacgcgg tgagttgtat cttgttgagg gtgactcggc aggtggtaca gccaagcaag
    19501 gtcgtgatcg tacttttcaa gctatcctgc ctctgcgtgg aaaaatattg aatgttgaaa
    19561 aagcactcga ctggaaaatt tttgataacg aagagataaa aaatattttc acggcactgg
    19621 gtgttatgcc ggcagaagat aaaacgttgg atactaccaa actgcgctat cacaaaatca
    19681 tcatcatgac agatgccgat gtcgatggta gccacatcac cacacttatt ctgactttct
    19741 tctttagaaa atgttatgag ttagtgcagc aaggatatat ttatattgct actcctccgc
    19801 tgtatttagt gaagaaagga aaagatcaac gctattgttg gaatgaagaa gatcgcaaag
    19861 cagcagtggc tgatttttct aaaggcggaa gcgaaagttc agtgcacgtt caacgataca
    19921 aaggtcttgg tgaaatgaac gcagagcaat tgtgggaaac aactatgaac cctgaaaccc
    19981 gcaccctgcg ccaagttaca attgatagtg ccgctgaagc agatcatatt ttcagtatgc
    20041 tgatgggtga agatgtacca ccacgccgcg aattcattga ggcgaatgct aaatacgcaa
    20101 acctggatat ttaatgcatt tctaataaaa gaaaagcgac atatctttcg gtatgtcgct
    20161 tttttattta attatttttt tgtgatttta caatccaata ttgaagccca ttctaacaac
    20221 ccatggacta taatatatcg attcgatatt gtgcaataca tcgtataata acatgatttg
    20281 gaagcgagaa tttgatccaa cttcgtaatt atatcccatg cctaagaata atgctgatac
    20341 gtcattggtg tattcgccat aggttggatc ttcataagta aatttgaccc atgaatgctc
    20401 gaattctgca tgcgcaaata agccataaat taaatcgtgt tgcacaaaaa ctctcggtcc
    20461 caacacttga gctttgtaat cggcaaaagt tgcaaggttg taattttcgc taaaatattg
    20521 ataggtaaat ccaacaccgg cggttagcat ttctgtaatc ctgtatccga caattggcga
    20581 tgcctcaatg taggtggcct gtccaaattg tgcacccaga ttaccgccta caacaatatt
    20641 gtctacccaa ttctttttgt catcacctga atctccatca tcatttgaac tttcttcatt
    20701 tgttgtttcg gcatctgaag ggtcaatagg ttgcgtttga gcctgaacaa tttgtaggct
    20761 gcaaatcagg aatagactgc acaggaagcg tttaaacgat atcatatggg tggtaaaatt
    20821 agcgagaact cattttaatg aagtacaaag ccttgaaatt tagggtggat cgggaatttt
    20881 gagtccctaa acgcctattt caccgcaaca attgttcctg aaatgttatt ccgtacatta
    20941 gataggtgat ttcaccccaa aaaacaccaa ttttgctaga aatcggttgt gaacctatgt
    21001 atacacctgt agttttatcc tcagaaatac catccatgaa gctgttaaac aaggagcagg
    21061 tagactggcg tctgattctg tttgtgctat tactcgtgct gctccccttt attttattcg
    21121 cagactcggc tgcaaaatca ccgctccctg agggtgcaac tgaccctgca ttcaacatcg
    21181 agagcactcc cgacaccttg aaggaagcct ttccagctct ttattccagc ctgaaaattt
    21241 ctttggctac tattccaact gatacggttt atgttattat tcaccccggc gatcaattaa
    21301 atgtggcagg aggttttggg ttgaatgaca ccttgcggtt tactccttac ccaatggccc
    21361 tttcacaaca agaagtgaag gtgaagccct ggaacgacct aattcttgaa ggttggcact
    21421 ttgggacgct aacatttgaa attgttactg atgcagttga atatgcgggc ttggcaattc
    21481 cggacatcag ttatacgata gaggataatg aactgccacc gggaatcgtg caagcggtgg
    21541 atatggatac aacactcaca gagggattaa caggcattga tttgttgttc tccatggcaa
    21601 ctatacctac agatactgtt ttcctaatta tagatccgga cgatcaatta cgcattaccg
    21661 gaatacccgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcagattat
    21781 caagcattcc gactgatacc gtttttataa cctttgaccc ggatgcacaa ttgcgagtta
    21841 caggcattgc agatgtacct gtgacagtca tatatccacc aacagctgct gcactcgcat
    21901 ttgatggagt gtcattaagg gcttatgatg attatatttt tgaaggagac catagcggga
    21961 caattcagat atccatagaa tcaaatgatc cgtactatga tgcaatgtct ttgcctgata
    22021 tgacatttcc gattatcgat aatgacaatc ctcccggatt aacatattct attccggatg
    22081 tgcttgaact ttcggaaacc ggcggtgaat taccaatagt agttgcattg cagtcggtac
    22141 cgacagatac tgtaaaaata ttggttgatc cgagtctgca gttgcgaata actgatcccg
    22201 gagttggtgt ttatatgatt ttccccccaa atgcgagtgc acttaatgat cacatcttaa
    22261 atattaaggc tgtagatgat gccgatttcg aaggcacaca tacaggctct ttcagcttta
    22321 attttgaaac tattgatccc gattatgcat taatggatat tcctacgcaa acggtaacca
    22381 tttttgacaa tgaccttgaa ccgggtatta tcttcctcga cacctttggt ttggccgggg
    22441 tagaaggtga tacttcatta gatatcttga ttaaactaaa cagtattcca ttaaatcccg
    22501 tgacgattga acttacaccg gatgtgcaac tcgatctggg taaaggacct ggtggactgg
    22561 tgaaatataa attccgcagc gactcggcct tgatctttca gactgttcgt attaaaattg
    22621 tggatgacca cttgattgaa ggtgatcata agggttacgt ttcagtaaat attactaccg
    22681 aagatccata ctacatgatc atgagtgtgc caacctttga tgtaaatatt acagataacg
    22741 atatcgttcc ggtttacaat tacaacccac ttgttttcaa tgcttttcct tcggtcgctg
    22801 gtgacttgat caatattcag tcgcctgaaa atggcaaaat tgatgtcatc gatgctaatg
    22861 gaaagtatat gttcggtatt gaaaatggca taggttctac ctcgatagat acccatagct
    22921 ggccttcagg aaattatatg gtgatatggc gtagtgagga aaatgcctac tatgcaaggt
    22981 tcatagtgct tcattaagct gtttttcaaa tatggtgaca ttagtcattt ttgcgcgata
    23041 gcatcaatcc tagctttggg gcatattcaa gcccatgcag gatcatcaac atgctacatc
    23101 tgtgcttact tgctaccact gcggtgagca atgtaaggat actacttttg agatagagga
    23161 taaacacttc tgttgcttcg gatgcaaaac tgtgtatgag attttgcagg ataacgatat
    23221 gtgtgcatat tacgatatga ccgcacatcc gggtgtgcag caacgcaata aaacagatat
    23281 ccgtaaatac gctgtactcg atgatcaaca agtgcgtgca aattttgttc ggtttgctga
    23341 tggtaaccaa acgcatgtta cattttatct ccctgccatg cactgcagtt catgtatatg
    23401 gctgcttgaa cacatgcata aattgcatcc cggaataatt caatcacgtg tcgattttcc
    23461 taaaaaagaa gtttctgtcg tttttgatga acgtgaattg gcattgagta aagtcgccgc
    23521 attactcgac agcgttggtt atgagccatt gttgagtatg agcgacatgg ataaaaaacc
    23581 tgaaaaaaca ttacagcgct ctcgactaat taaaattggg attgcggggt ttgcatttgg
    23641 caatgtgatg atgctttcct ttcccgaata tctggcaggc ggtgaagtga ccgatgcagg
    23701 attgcaaagt atgttcagat ttctttcact cgcattggct ctccctgtat ttttctattg
    23761 cgcgagtgaa ttctatgttt cttcatggaa gagcattaaa aataaattcc tcaatatcga
    23821 tgtgccaatt gcactggcga taattataac cttcaccaga agtctttatg ccatttttgt
    23881 agagcatggt cccggttatt tcgattccat gaccggaatt gtattcttta tgttggttgg
    23941 aaggtatttt caggacctca cttacaaaac aatttccttt gaccgcgatt atcgcagtta
    24001 ttttcccctt tccgtttcgc tgttgaaaaa aggtgaagag gtagaaaagc cactatcagc
    24061 gctcgaaaca ggtgatctta ttgttgtaag aaataatgaa ctgattcctg ccgatgcctt
    24121 actggtgaaa gggaaaggac aaattgatta cagctttgta accggtgaat ctgaaccaat
    24181 tgaaaaacgc attggtgaat tagtttatgc aggcggacga cagacaggcg cagctattga
    24241 attaaaagtg gtgaaggaag tatcgcaaag ttatttgacc caattgtgga ataatattga
    24301 tgtttcacgc aaggaagatg aacgtaaatc ttttgttcaa atgctcggta gatattttgc
    24361 cctgattgta ctcatcgttt ctgcaattgc ctgtggatac tggatcatgc agggcgatta
    24421 tcaacgtgca cttgattcat taataacccc catgattgta gcctgtccat gcgcattatt
    24481 gttatcggca acattcacca atggcaatgc catacgctat ttcggtaaat acggtatcta
    24541 tcttaaaaat gcactggtaa tcgaaaaaat gaccaagact gatgccattg tttttgataa
    24601 gacaggcaca ctcactcaat caaatacaac atcaacagaa tatatcggcg aggagctttc
    24661 aagtgtagaa aagcacatga tagcagtagt ggcagcgcaa tctacacatc cttacagtaa
    24721 agctattgtg cgttttttgg gaaatacgat gttgtatgca gtcgagaact ttaaagagtt
    24781 tacaggggct ggaattgaag gaatggttga tcaggtgcat attcaaatag gttctgcaaa
    24841 atttgtaggg gttcctaatg cccttcagga agaacatggt attgcataca ttgccattga
    24901 taatgtggtg agaggcagat tccgttttgc gaataaatac cgtgcaggtc ttgatgacat
    24961 gatttcaaga ctaaaacagc agtatgatgt ctatatttta agtggcgata atgatgcaga
    25021 caaggaatat ttgcttcaat attttcctgc agaacatatg ttcttcaacc agtcgcctca
    25081 cgataaactt aaattcatta aacaattgca acagcgccat cgcaaggtca tgatgttggg
    25141 tgatggcttg aatgatgccg gagcattgca acaaagtgaa acaggtattg ctgttgctca
    25201 aagcagtaat ttcacacctg ccagcgatgt ggtaatggat gtaaaacaat tgattaatct
    25261 gcctgcgttg ttcaaatatg ctactgctgc tcgacgtatc attataggta gttttgtgtt
    25321 aagcatcatt tataatgtga ttgggctgta ctttgcattg caaggatcac tttctcctgt
    25381 agtcgcagca atattaatgc cattaagtac catcaccatt gtttcattca caacaggtat
    25441 gaccaccctt gcagctaaat gggtaatggg taaaaatcga gcttcctgat aagcaaaatc
    25501 ttcagtgatt ctgtgccggc aattgacaat ttattatttg tctaaactgt cattttttgc
    25561 attccgtttt ttgtcaaaag acttgctgcc taagcgtttc atcaaattta tggatttgac
    25621 cccttcgtca aaagatgata aaagtcacct tttgtcgtga agtatgtcat ctgtgtcgga
    25681 tttggttatt atcaacttag cgtctgatta tgagtgtaat tctgatattg ataatagcaa
    25741 gtgtactggt agctgcaggt tttctgtttg cattcatatg gtcggttaag aaagggcagt
    25801 ttgaagatga tatcacccct ggtatccgca tgcttttcga cgacgaaaaa tatatagaga
    25861 aatcacattc aaccaataaa cctaaatagt taattatgga tctcgtaaaa gtcagttatg
    25921 acaacaagat tgtgaaatgg tttgcgatag caactgtttt ttggggaatt attggcatga
    25981 ccgttggttt gtggatcgcg ttagaactcg tgttcccaca actcaattta ggtgtgccgg
    26041 tattaacctt cagccgcgac cgaccaattc acacgaacgc cgtgattttt gccttcgtag
    26101 gtaacggtat cttcatgggc gtttattatt cattgcaacg cttgtgtaaa acacgtatgc
    26161 tcagcgattt tctcagcaag cttcacttct ggggatggca gttgatcatc gtgcttgccg
    26221 cactttcact cagcatgggt atcaccaccg gtaaagaata tgccgaactt gaatggccta
    26281 tcgatatttt gatagcattg atttgggtgg tattcggtat caatatgatg ggtaccatca
    26341 tcaagcgtcg cgagcgtcat atttatgtgg gtatttggtt ttatatcgca acatttgtta
    26401 cggttgccat gctgcatatt gttaactctt tagagcttcc aatttcactt acaaaaagtt
    26461 attctatata cgccggtgtg caagatgcgc ttgtgcaatg gtggtatggt cacaatgcgg
    26521 ttgcattctt ccttactaca ccatatctgg gtttgatgta ttacttctta cctaaagcag
    26581 ctaacagacc ggtttattca tataagcttt ccatcgttca cttctggaca ttgatattct
    26641 tatatatctg ggctggtcct caccacttat tatatactgc attgccacac tgggctcaaa
    26701 cattgggaac cgtattctca gtaatgttga ttgcgccatc atggggtggt atggtgaacg
    26761 gattgcttac gctccgcggt gcatgggata aagtacgtga agatgtagta ttgaaattca
    26821 tggtggttgc cgtaactgct tatggtatgt caactttcga aggcccaatg ctttcattga
    26881 aaaatgtaaa tgctatcgcg cacttcaccg actggatcgt ggcacacgta cacattggtg
    26941 ctctcggttg gaacggattc ctcaccttcg gtatcctgta ttggttgata cctaaaatgt
    27001 ggagaacaac actttactct aaatcattgg caaatctcca tttctggatt ggaacattag
    27061 gtatccttat ttataccgtt ccaatgtatt ggggtggttt cacacaagcc ttgatgtgga
    27121 aagatttcac acctgatgga caccttacat attcattcct cgaaacaagt acacaattaa
    27181 aaggcatgta tctgatgcgt tcaattggtg gtaccttgta tttagtaggt gtattcatga
    27241 tggcatataa cctggttaaa acagcaaaac aaggttcatt tgcaaataat gaagatgttg
    27301 aagtgcctga aatggaacgt gtagtgaaag tacctaaaaa tgaattctgg cacagagcac
    27361 tcgaacgtcg ccctgttcaa ttcatggtat tggcacttgt catggtaatt attggtggtg
    27421 ttgtagaatt ggttcctaca ttcctggtga aaagtaatat tcctaccatc agtgcagttc
    27481 aaccatatac accgctcgaa ttacaaggtc gcgatattta tgttagggaa ggatgttatg
    27541 tatgccacag ccaaatggtt cgtccgttcc gttcagaaac tgagcgttat ggtgaatatt
    27601 ctaaagccgg tgaatttgtg tacgatcacc cattccagtg gggatcaaaa cgcacaggtc
    27661 cggatttggc tcgcgaaggt gtgggctggt tgaaaaaatc agactcatgg cattatatgc
    27721 acttgctcga tccacgatca ttgtcggccg gatcagtgat gcctgaatat tcatggctgc
    27781 atgaaaatga gttggatatc acaaccactc ctgctaaaat cagagccatg caaaaactcg
    27841 gtgttccata tcctgaaggt tatgatcaga tcgcaaatga cgacctgatg aaacaagcag
    27901 aagagatcgc cgccagtttg aagaaagatg atatcgagac tgaacctact gttgaggtta
    27961 ttgccatcat tgcatacctc caaagattag gtaaagacct cgaaacagaa aatgcattaa
    28021 atgcttctaa cacaaaataa tctgctgata tgaacttcat ggcttattta tcagatatca
    28081 acggcgtaag tatttacccg attatatcgc tgattatttt cttcgcgttc ttcatcggac
    28141 tgtttgtctg ggtaagcaga atgagtaaaa gttctgtcga cgaatgcaaa cagattccgc
    28201 tggaagacaa tcaaccaatt cactcaactt caaatcatca gtgaaatgaa aaagctgatc
    28261 ctaaataaac acctgatggt tgcccttggc gctgtgttaa tgacaacacc acttatggcg
    28321 caaaccactc ctgctcctga aaagaactgg tatgttcacg acggactatt ttggctattg
    28381 atcgcagtag cagttatcct gttatatgtg atctatgcat tggctgaagt agtcatctgg
    28441 ggcgcaaaaa agaaaatctc cgatcgcggt aataccacca aaatattagc attggtaggt
    28501 actgctgtta tgttaatgtc gggaaattca ctcatggctc aagctgcttc tgctcctgct
    28561 gcggcacctg ctagtggcct gttgcaaagt gactacctgc cattatacat tctgatagct
    28621 atcgaagtgc ttgcaattgc atatatgagt atcatgttgc tgcaactttc gaaaaaagaa
    28681 caactcgaag ctaccggaaa agcagaaact tggttatcgc aattgtggga gaaatggaat
    28741 tacaaagtgc caattgagcg cgaagatgaa atgttgatct ccgaccatga ttatgatgga
    28801 atccatgaat tggataacgg tatgccacca tggttgcaat atatgttctt ctttaccatc
    28861 ggcttcgcaa ttgtttactt gtggtattat aacatcggca acggcccgac acaaattgat
    28921 gaatacaatg cttctgtaga aaaagctgaa attgaaaaag cagcttacct cgcagcagca
    28981 ggggccaatt atgatgaaaa tactgtaatc ctttcaacag atgcttccgt attggctaac
    29041 ggtaaatcta ccttccagca gtattgcgtt ccttgtcatg gtgctaacgc ggaaggaact
    29101 accactgcgc caaacctaac cgacgactac tggattcacg gcggaaaaat caacgatctt
    29161 tttaaaaccg tgaaatatgg tgttcagggt aaagcaatgg caagttggca ggaagtgctt
    29221 gatcctaagc agatatttga agtaacaaac tatattaaat cattacatgg ttccaatcct
    29281 gcaaacgcaa aagctccgga aggtgcattg tatgttgaag gtggagaagc tgcaccggca
    29341 gacacatcag cggtaactcc tgatagtctt actgtgcaaa atccggcaac tactgtaatg
    29401 caataaataa caatcaacgt cggtctgttc gcaggctatg aatagaccga cgttattttt
    29461 tttcaggcag aagaaagaaa agtgttatga aagacgataa gtcgttccgc gacatcatct
    29521 acacagcaga tgaagaaggc catcgcaaat gggtcttcgc agcaaaacca tcaggtagat
    29581 tttataactg gagaacaatt atcagttata tttatctgat tgcatttttc gccctccctt
    29641 ttatcaaggt acatggcgaa ccattattcc tttttaatat tccggatcgc aaattcatcc
    29701 tgttcggcgt aattttttgg cctcaggatt tttatttgtt tgcactcggt atgctcacct
    29761 tcctggtatt catcattttc tttacagtac tctacgggcg tttgttctgc ggatggattt
    29821 gtcctcagac agttttcatg gaaatggtat ttcgcaaaat cgaatacctg tttgaaggaa
    29881 ctgccgaaca tcaacgcaaa ctggcaaaaa tgcaatggaa tacagaaaag atattgagga
    29941 aaggcggtaa acaagtggtt ttcttcctct tctcattttt agtaggaaat acttttctcg
    30001 cttatataat aggtgtagac cgactctacg agatcataac cgaaccactg aaggaaaatg
    30061 tgggtggact ggctatcatg atggtgttct catttgcatt ttttattgtt tttgcattct
    30121 tgcgcgagca gatttgcacc ttcgcatgtc cttatggtcg tttgcagggc gtgctactgg
    30181 ataaattctc gatagtagtt gcatatgact ataaacgcgg tgaacctcgt ggacatcata
    30241 agcatggcga agaggataaa caactcggcg attgtatcga ctgcggtttg tgtgtacgcg
    30301 tatgtccaac aggaattgat attcgcaacg gaactcagct ggaatgtgta aactgtactg
    30361 cttgtatcga tgcatgtgat tctatcatgg ataaaatcaa caagcctcgc ggattagtgc
    30421 gatattcttc tgagaacaat attgtaaccg gtgagaaatt gaagttcact ccgcgcatga
    30481 aagcctattc actggtattg ctattattag tgagtgtgct tacaacttta ttggctacaa
    30541 gaaaagatgt gacagctaca gtaacgcgct cttcaggaca attatatcaa aaacgagata
    30601 gcgttttggt tactaatatt tataatatta aagtggcaaa taaaacgcac gaaagtttcc
    30661 ctatccatct gaaattggaa aatgttgacg gaaaagtaga aatgatcggg aacgaaatgc
    30721 atattaattc ggaagacctt gcagaaggta aattctttat agtgttatca agagatcaga
    30781 tacataccat gaaaatggac gttcagatcg gtgtttataa tgatgataag aaaatcagta
    30841 cagtaaaaac aacttttttt ggacctgttt ctaaaaagaa ataactatga attggggtag
    30901 aagtatttta ctagtttatg ttggctttgt tgcactcatt atggtgatgg tgttcaaaag
    30961 ctttaacaat ggttttgatt tggtatcaga ggattattat gccgatgagc taaagtatca
    31021 ggaccgtatt aatgcaacag aaaatgcctt gccatttgcc gattctattg atgtggccgt
    31081 aggaaacgaa tccatcgata ttcactttcc ggcatctctt gcagctatgc aaagcgggga
    31141 agcatatttt tacaaaccca gcgatgcaaa gcaagacctg cgttctccaa ttaaaatgaa
    31201 cgactcaggt gttcagcaat ttgcaaaaag tgaattttcc gcaggtgtct atcgtttgaa
    31261 aatgacctgg accatgcagg ataaaaccta ttacgtagaa aagctgatta aattgtgaca
    31321 gcacttctga tcacggcatt tattaccggt ttatttggta gcctgcattg cgccggcatg
    31381 tgcggaccaa ttgcactgtc gtttaatatg caaggaaaag gaaatgccgt gatcaacgcg
    31441 ctgttatata atgcttccag aatttttgct tatgcactta tcggtatgtt atttggatcg
    31501 ctcggcgtta cgtttcacta tgtaggatta caacaagttg cagctattag catgggcgta
    31561 atgattttga ttatcgcttt aattacttgg ctgggagccg cttcatctgt taaattaccc
    31621 ttgataaaag aatacacaaa ttttatccaa cgcaaaatcg cgcaagcatt taaatcatca
    31681 agtccttctc gtattgtggc attaggtttt tttaatgggc tcttaccatg cggactggta
    31741 tatctcgctg tagcaggcgc tattgcatca gggaatactt ttgtgggtgc tgcgtatatg
    31801 ataatatttg gattaggtac gctgccttta atgttcattt tctccgcctt cggaaactgg
    31861 ctgggattca ggcagcggtt atttatcaga aaatacctgc ccgtattcag catcacaatt
    31921 gcaattctcc tcatcctgcg tgggcttaat ctcggaattc cttatgtaag cccgcaattc
    31981 actcatgatc agcataatcc tgttcccgct gatcaacatt gtagtccagc agcgcagcaa
    32041 gaggcgcata ttcttcaata agtataaatc ttttcctccc ggcattttat accttcgcag
    32101 gtatgactgt agccgatatt acacattatt tggaaacgat agcaccacta tcgttgcagg
    32161 aatcatatga taacgccgga ttgcaaacag ggcatccgct gcaggatgta aatgcaatcc
    32221 tgatatcgct cgatgttact gaggcagtgg tggatgaggc cattgcgcag ggatgtaata
    32281 tgatagtcgc ccatcatcct gtagttttta cgggtttgaa gaggcttacc ggaaaaacgt
    32341 ataccgaacg cattattatc aaagctgtgc gcaacaatat tgccatctat gccattcaca
    32401 ccaacctgga caatgttgag acgggcgtta atgctatgat cgccgaaaaa ttggggttgg
    32461 tgcacggtag ggtattacaa cccaagcccg gattgctccg aaaactagtg acatttgctc
    32521 ctgaagcgca tgcaggtgag gtgcgacaag cactttttca agccggcgcg gggcatatcg
    32581 ggaattatga tcattgcagc tttaatgtag atggcgccgg cacctttcgg gctgatgcta
    32641 cggcaaatcc atttgttggc aaaagagggg tggaccatga ggaagcggag accaggatag
    32701 aagtcattta tccgcagtgg gctgaaactg ccgtgcttcg tgcgctttta aaggcccatc
    32761 cttatgagga agtagcctac gacctttacg cacttggcaa caatcataag ggagtgggaa
    32821 gcggatggat tgcagaattg ccccaggcta tggaagaatc tcaatttttg gccatggtag
    32881 ccggaaaaat gcaggcaaaa ggagttcgat atacggcgct tttgggtcgc gatatcagga
    32941 aagttgcgct ttgtggcggt tccggaagct ttttgataaa ggatgctatt gcagccggag
    33001 ctgatgcttt catcaccgca gacgtcaagt accaccagtt tttcgatgcc gaaggacgca
    33061 ttttgctggt agatatcggc cattttgaaa gcgaacaata tacctcagac ctgctggcaa
    33121 gggctattag cgaaaaattt gctaactttg cagtccgttt gtctaacgtt aataccaatc
    33181 caatcaacta tctataagct atggctgttg cgaaagaaat gtcagtaaaa gaaagcatgc
    33241 aagcactctg gaaactccag agaatacatt ctaagatcga tgatatcact accctgaagg
    33301 gcgaattacc catggaagtg agcgatctgg aagatgaaat tgcaggtctt gaaacaagga
    33361 tgggaaaact ggagaatgaa ttgaaagata ttgaaacaga gatttcaaat cgcaaaacaa
    33421 ccatctccaa ttctaaagct cacattaaaa aatatgagca acaacagaat gcagtaaaaa
    33481 atagccgcga gtttgatgcg cttacaaaag agatcgaatt gaataaactc gatatcgaat
    33541 tgagcgagaa gaaaattcgt gaagcaaatt ttgccaaaga agcgaaatct gttaccatcg
    33601 aagaagcgaa agtgatcctt gataataaga agaaagatct taaaaagaaa aaagaggaac
    33661 tcgaacgcat catcggtgaa actgaaaaag aagaagctga actcgctaaa aaagtagaga
    33721 aagcagaaaa agacctcgag ccacgtttga tccaggctta ttatcgcatt cgtgaatcat
    33781 atcgcaacgg acttgctgtt gtttcagtag cacgtaattc atgcggtggt tgcttctcag
    33841 ttgtgcctcc acaacgtcag gcagagatca aacaatcaaa aaatatcatt atctgcgaac
    33901 actgcggacg catccttgtt gatgagtcgg taatggaagc ataatataaa ctaaaacacc
    33961 aaaatgccat gaagcaatat atcatcgtat tgttcatggc atttttcatt cccgcaagcg
    34021 ccatcgccca gaaatatgat ttcaaccctc gttgtgttga agcatatact tacatacagg
    34081 agcttcgtct cgaaaagggt agggcgcttc tcgacaaaga gaaaaaagaa aatccatcca
    34141 atctcattcc ctacctgatt gaaaattaca tcgatttttt aactacttat ataaatgaag
    34201 accccgatca gtttaaatct cttgagaaaa ataaatctac tcgcctcgac aaactgaaaa
    34261 ccggcgatca aacctcaccc tattatctgt attgtcaggc tgaagtaacc atccaatggg
    34321 cgttttccaa acttaaattt gaagaatatg taggggcctt taccgatgtg cgaaaggcct
    34381 atttattatt agaggagaat cagaaaaaat tccctgattt tattgctaat aataaaagcc
    34441 ttggtatgct tcacgccatc gttggtgcca ttcctgatca gtataaatgg ggtattaata
    34501 tgctcggtat ggatggttcc attgaccagg gattgcggga attggaatca atattagaat
    34561 actcagaaac gaatactttc gtgtttaaac aagaggttta tctctattac gcatttatag
    34621 ccttatacct cggtcatgat gcacaaaccg cctgggatat tgtgaaagac ctcgacacaa
    34681 aaacaaatct catcaatgta ttttgtaaag ccagcgttgc tatgcgaaca ggaagaaatg
    34741 atctggcttt gcagacctta cagtccagac cctcgggaag tgaatatttg ccatatcact
    34801 acctcgattt catggtaggc cttgctaaag cgcgtaaatt agattccgat ggtgcgcaat
    34861 atttcgagcg atttctgaaa aactataaag ggaaaaatta catcaaagaa gcttaccaaa
    34921 agttaggatg gctggcttta atcagaggtg ataaagataa atattgggaa tatcaatact
    34981 atgtaaaatc gcgcggtgat gatctcatcg atgatgataa gcaagcatta tatgaagcag
    35041 aaagcggtca tgtgcccaat gttaccctat tgaaatcgcg actgttattc gatggcggct
    35101 attataaaga ggcattaaaa ttattggaag gaaagagcgt agatagcttt tccgataaaa
    35161 aagacaaggt tgaatttacc tatcgtgccg gtcgcattta cgatatggca aaacagcccg
    35221 acaaagccaa aggtttttat ctggctacca ttaaaaatgg cgagaccttg ccatactatt
    35281 atgcagcagc ctctgcatat tatctgggta ttatcttcga aacacaaaaa gattatccca
    35341 atgcccgcaa atactaccaa aagtgtgtcg acagcgacaa caaagaatac caaaccagtc
    35401 ttgacggcct ggccaaagcc gggttgaata gggtgaaggg gaagtgagtg tttatactgt
    35461 gtatctcctg ttgccctaat ttaatgaatt tgaaagtagt gtttgattcg gaattattat
    35521 tccttaataa atgtagcgct tttgaaacta ttttctgaat aaactgctaa agtatacata
    35581 cccggactaa ggttgctcac atttatcaat ccttgatgca tgaccggttt ctgcaccaat
    35641 cgcccttgaa tatcaaaaat aaacatttca gtatcatcct gaaccggaat tcctatatgc
    35701 aaatagttga gcgttggatt tgggtaaaca tataaatgtt caaccgcaag ctgattaatc
    35761 gctacgtcca aactgctgaa gtcataaggt gtcccccaag taattaaacc cacttgaaaa
    35821 taaaccatgc ttaattcata atatgcatcg gctgatagtg aatcaagata ataaatttgc
    35881 cccaggccat caccgacaat ttcaaccggt tgactgatat tatcagggtt tgaaaggcaa
    35941 tatgtggcat cgttgtaaac ataatgatca tatacatttt tatgcgggac accttcatag
    36001 agactggtgt ctttcaccca atctgagtaa ccggcatgta tatgatcgaa aagcgtaaat
    36061 tcgtgtgcat ctaaatatgc aaaatcggca taattaaatg ttaattcaat tgtgtcccat
    36121 ttcaagttga ccgatggaac gccatcgtta taaagggtgt catattgcac acgctctact
    36181 tgatagctga caccatcggg tagaggtgtt ttagtctgga taaagtactt tattgcgctt
    36241 atacgtttat cggcatcact ttgattatca gggactgatg tttctcgata gtgaaattca
    36301 tatcccaagg aaaaattata tgctctgtaa gcatctacat caactatgtt ttcatacgga
    36361 ttcgtaagtc cgcgaagcac tcgtccaata ctatctccgg gaagaggaaa tatattgaaa
    36421 ttaaaaaatt cagtgagccc atgaaattta gaaatatcca tttttgtttc tgtagggaaa
    36481 ctatctggca tgatgacatt taatgcattg aaaactgtga attgtattcg gagaatggta
    36541 tcttccacat cgggtaaaat tgttcgctcc aatttattta caacggtagc tttaatgaat
    36601 gaaccatcag gccatttgta cacagtccaa tagtcaccta tgtttacaac cgatttaata
    36661 aatatgctat cgccatgttg attaaaaaag acatgcgtag cttgtgcatc aggtttgatc
    36721 agcattttaa atcctaagat gcttgtatca gtgcgtaccc attcgcaatc cgggtcacct
    36781 gctacatttt ggaattgtgt actgaaataa taaacagaat caagcccttc caatgccaca
    36841 gaatctgttt taataaaata gaatagacta tccttgccgg ttactgaaag ccttgcaaaa
    36901 gtcatttccc tgtccttgtc aaccaatctg taattctgac tgaaagcatt ggtgcttacc
    36961 atggaaataa tacccgtaat cagtataatc gcaagccgaa cttggttttt gagcatgttg
    37021 aaaagtttac tgagtggcga tagccgaata cctaaattta cgtaaaaata ctaaaagtta
    37081 cctgttaaag ggcatgccgg gcaggctagc atacttgtaa tgatggttca aattcaacac
    37141 aacgtctctt taaaaccctt caataccttt ggaatagagg tgcagacgtc ggcgtttacc
    37201 agtatcaaaa cggtatctga ccttcgtgaa ctcattcatg caggcacttt gcaatcaagc
    37261 catatgtttt taggtggcgg aagtaatgtg ctgtttacca aagactttaa tggacttgtt
    37321 atcaagaatg aatgtaaagg gatctatatt acaaaagaag acaataggca tatatggatt
    37381 accgcaggaa gtggtgaggt atggcatgat ctggtaatgt ttaccgtgac gcgtggattg
    37441 ggtggaatag aaaatatgag tcttatccct ggtacagttg gtgcagcgcc aatgcaaaat
    37501 ataggcgcat acggtgttga acttactaat gtatttgaac aattgacggc tatcgatctt
    37561 ctaacaggtg aggaagtatt attcaatcac gagatgtgtg ctttcggata cagagaatcg
    37621 gtatttaagc atcaattgaa aggaaaatat tttatagctg atgtcacctt gcgtctttcc
    37681 aagtatccgg aactgcaaat caattatggc gcaattaagc aggttttggc agaaagggga
    37741 attgttgaac cgacaataaa agacatttct gatgcagtaa tttatatccg tcaaagcaaa
    37801 ttgccgaacc ccgtagtctt agggaatgcc ggcagcttct ttaagaatcc tgaaattgca
    37861 gttgaacaat atgatgaatt gaaacaattg tatccaacaa tacccggtta tcccactact
    37921 cctaataaaa ttaaagtacc tgccggatgg ttgattgagc agtgtggttg gaaaggtaaa
    37981 attgtcggac atacaggagc tcacaaagat caagccttgg ttttagtgaa ttatggaggc
    38041 gcaagtggta atgaaatttt agcactttcc aaggaaattc aacaatcaat actggccaaa
    38101 ttcaatatcg caatctctcc ggaagtaaat attatttgag tttaaggtca gtatacgttc
    38161 atcaaagctt taggaattta cctgcataat tcaagttatc atgttttaca aaaacaacat
    38221 acatacctga agctagatca ctaatattaa taacaggatt gtctgcaggg ccgcgaccta
    38281 caagtgaacc atattgattg taaacctgat atgactcatt ttcattgcat gctattggca
    38341 ggataattac atcagatgtt gggtttggga gaagttgaat ttcaattaca gctttattcg
    38401 atgcgccaaa ttgagatgcg aaaatttcac ctgaggcgtg tcctgagaga attgaaccaa
    38461 cgagcgtttg agtataaacc catccgtaaa tatttgcata ggaagaaatg agtcctatac
    38521 cacttgaaat agaataagtt gtaggtgaat atgcgtgccg agtacaatcc acagtatcaa
    38581 cataagcttg tgttaaagaa tattctatgc atgttgcatt ttcaaacctt gttgaattga
    38641 tattataccc gtcacttgaa ctaatataat agaatccgtc ttctaggcca tcagttagtg
    38701 tagggtgatt tccttcaaaa gcgccataat ggtatgaact gttataaccc tcaaataatg
    38761 cttgaaccac acttctgcta aatagatttc gtccgggata aatatcattt ctaatgaaat
    38821 taactacaat tgagtcagga tatttgtcga cagatgtgat tttatctacg aagtaatcta
    38881 catcatttac accatggctg gatatgattg tgcttgagct atatactaat atgtcatccg
    38941 gctgcaattg aatataatcc gaccatttcg gaatggttaa gcccagggtg tcatcagttt
    39001 gaataaatcc aaccaagtct ttcatcagca gacaatcagg acagaacacg ccattcacga
    39061 gcctgccaaa attcggaaat gaaagcagtc catggttctt tgacaaggta aaaacattgc
    39121 cttcgaatat cgttccttcc tcaggctccg gagaaactcc aattgaataa tagcgaacgc
    39181 tgtcaaaatc tccaagtaca ttaatatagt cgattgaatc aaatgtaact gtcaattcat
    39241 catataatga agcgggattt acgatgggga cagcccaaaa atcacctaac ccaatgtcag
    39301 tattaaaata aaatccgttt gtcgaatcaa tgttattgta aatcagatat tcatttccgt
    39361 tacctgtgaa atatatttta ttgcctttat atggaatgct tgcattaaga agatcgtaac
    39421 aatgttgcca tgatgattta ggagctttag cattgcaaaa atagatagta tttaatccgt
    39481 caaagttgat tgagtcgtta cataataccg agaatctggt ggttgatctc tccagatatg
    39541 aaagtttagc gccatatgga aataaatccc agttttgagc tttaccacta ttagttgtga
    39601 cgataattga tagtagtatg gtatacaacc tcgttttcat agacaaaata aagataattg
    39661 attatccaaa tttattgtca catctaggta aataatacct tagactgtct attctcaata
    39721 tttagcgcct tagcctcttc gcgtctttgc gtgacactag acacacctgc ttacttgcta
    39781 accggcaaac ttgctaactc cggcaaaaat ctcaatgagt ataccatttc aatttcacat
    39841 cctattctct ttcctctctc ctctctcctc tctccttcct catcagttcc atcatatccc
    39901 aaagctgagt tggtaactta tcaagcatgt taaactcgcc ggctcctttg agccattctg
    39961 cgccatcgat tacaacaact tcacctgtca tgaaagcagc aaaatcagat agcagatatg
    40021 ctgctaaatt tgccaactcc tgatgctctc ccaatcgacc aacggcattc ttttttgcca
    40081 tatcaaaatc atcttccatg ccaggaggta ccaatctgct ccatgcacct tcagtaggaa
    40141 atggaccggg agcaattgca tttagacgga tatgatattt tccccattca actgcaagtg
    40201 atcttgttag cgcaagaaca cccgccttac cacatgcact tggaactaca tagccgcttc
    40261 ctgtccatga atatgttgta acaatattta atacacttgc cgaaagttta ttttctatcc
    40321 aatatttccc gcatgcaagt gtgcaattat aggtgccttt taaaacgatg tcaacaacag
    40381 aatcaaaagc gcggtgactt aatcgttctg ttggtgaaat gaaattgcct gcagcgttat
    40441 ttaccagcat atctactttg ccgcatttct cgatcgctga ttgtaacatg tgttcaactg
    40501 catcataatc ccgaacatca caaactatag gaatacatgt ggatccggta gctgctgata
    40561 attcttctgc tgtttttgaa agtacttcaa tctttcttga agtgataatt actgttgctc
    40621 ctaattcaag gaaataccta gtcatgcttt ttcctaatcc ggttcctcct ccggttacaa
    40681 taattacttt gctggtaaaa gtttgtgcag gtaacatcgg ttgattatac atatattgat
    40741 ttttgtataa aaatactcat tctgataata catacctttg tgccatggca accttctccc
    40801 accaaatcaa agtccgcggc ttccattgcg atatgtttca acatgtgaat aatgcccgat
    40861 atctcgaatt tcttgaggaa acacgctggg aatggctgaa tcgaatgacc agcttcgaat
    40921 tctttgagaa acaccaaatg agctttgttg tggtgagtat cactatccat tatcgctggc
    40981 cgtctttact caacgatgtg ttaaatatta cggtggaaac gaatcatatt ggcaatcgca
    41041 gcgcttctgt aaaacaggtg attactcgcg atggcgatag caagctgata gcagaagcgg
    41101 aggtcacctt tgccatgatc gataaccgta caggcaagtc agtgcccatc tcggatgaac
    41161 tcaaagcttt ttttgaaaat tgatggctac ttcgcgcagc aattattgca gttgcgatag
    41221 ggctttttct aaactagcca tgctatcatc cacatcctgc aaactacaag ttcctacact
    41281 taatcggaac caaggactct ctttttctga accaaatgca taaaacggaa ctaaggcaat
    41341 cttagcctca ttcaaaagga aggatgcaac atctgccata ttttgaatca ccgttccatc
    41401 agccttcctc ttgccgcgga gatcacatct taccgtcaga taaatggcac tttgcggaga
    41461 aatacaatct actgcataac ccttgttttt caatgccgta aaatgatgat aaaatccatc
    41521 aagtcgctgc tgcacctgcg atttgaattg catcaaaaag ctatcaactg cagcatcatt
    41581 ggtaagaaag tgtgcactcg ctacctgctc agcctttggc gcccaagctc cgatatgact
    41641 tagaatactc ctcattttat caataatggc tttcggcccg aaactccatc ctacacgcac
    41701 gcctgttgcc gaaaatgctt tgctgatacc gtcgatataa atgacataat cacgcaattc
    41761 cggacgaagc gatacaggat cgaagtgctc agtgtcgcca aaagttaatg tccaatacaa
    41821 ttggtcgtat aaaatgtata gtggtttggc atctgccgga cgtcgggcat tctctgccaa
    41881 cactaaatca cagatttctt caagtgcttg tttcgaaaat gctgttcccg tcggattcaa
    41941 aggtgaacat aaagcaatca aagtggcatc ttgcacatgc ggagcaattt cttccgctgt
    42001 aggcatgaaa ttattttccg gcctcgtagc aacttcaact tttacacaat tacttaaatg
    42061 cgtgtagtga ttattattcc aactaggtac cggataaatt actttgtcac cggcatccaa
    42121 taaggtaata taggtagcgt aaatcaatgg tcggccacca cctgcaatta aatattcatc
    42181 gggtgaatag gagagaccaa gatgtttttc gacaaaatac tgcgccgctt ttcttaaaat
    42241 atcaatacca ttggcagctg gataatttgt ttcattgttt tgataggcag caataatacc
    42301 tttttctaat tctgcaggta ttggaaatat tttagggtcg aagtctccaa tggtaagatt
    42361 ataaatcttt tcaccttttt tgattctatc gttgacctcg ccggcaagct taatgatctc
    42421 tgatccgatc agattatcgg ccatatgcga caccttaagc gtgcgttgtg tggatgttat
    42481 accattgccc atagctgcaa attacgttaa tttttttcgt aaaactatca tgccaaaata
    42541 gctttgaaaa tgacaatata tgccactttc cgccaaataa agcctgtaaa gccttttagc
    42601 tgtggagaag ctgtgtattg aggccgaaaa cgctttcagg aaacgtatac cagatgctct
    42661 tttggtgcta ccagcttgcc aaaagatgtc aattggaggc caagtgtagc tgcataagac
    42721 ataaatgccg actcgaatcc ggctgtaacc gccaccaata agcctccgct ggtttgcgga
    42781 tcgcagagta tcgctttttg cgtgtcggtc agcggtgcaa ttttatgtcc atagctggcg
    42841 aaatttcggt tggtgcctcc cggaattgat ttttgtgata aataatgatc taaaatgctc
    42901 ctgtcaatgg ttggtacctt gtcaaacaca atttctgcac tcacactact gccttcgcac
    42961 atttcggtga gatgtcctag caatccgaat ccggttacgt cggtcatagc ctctatatat
    43021 tccaatttac caaatgccgc tcccggcttg tttaattgca tcatgctatc cggtgcaata
    43081 tcagcatgct ccggtagtaa aagatccttt ttttgagctg tggttaatat accgacgcct
    43141 aatttttttg tcagaaataa actacatcct tccgaagcac ctgcgttcgt ttttaattgc
    43201 tttaagtcaa caagcccact caccgccaat ccgaacacgg gctccggaca atcaatacta
    43261 tgtccgcctg caagcggaat acctgcctct gcacaagcct gacgcgcgcc atcgagaaca
    43321 agtcctgcat aacgcggatc taccttgtca atcggccatc ccaatattgc aattgccata
    43381 aggggtgttc ctcccatggc atagacatcg ctgatagcat ttacactggc aattttgccg
    43441 aatttaatag ggtcgtcaac aatcggcata aaaaaatcgg tagtgctaac tagtcccgtc
    43501 ccgttgccaa gatcataaac ggcagcatca tccttagttt cattgccaac aatcaaaccg
    43561 ggaaatactt gttgcttgcc tacagattgt aatatttcat ctaatatttt tggagatatt
    43621 ttacatccgc aaccggcacc atgcgaatat tgtgttaatt taattggagg taatgatgtt
    43681 gacatatggt attattctta agattttgtt gaaatatttt tttcggtgat ggcattcctg
    43741 cacacatgat caattaattg ttgtgcatta atttcaggat taattgttga catttcgatg
    43801 ttatgtattt cctgatgtgc acgttgtgtt aatccatagg catatgcttt atcataataa
    43861 cctaaaacaa tttccgtagc tttgaaaaga tcacctcgct caaagcattc aaccgctgct
    43921 ttcgcatgct gtccgcccaa tcgtttggtg atttttttta cggcaccaat taattgctcg
    43981 gcaggaaatt gaccataagc atgaatgaga aattgtatgc gttcatgaat tggcacatcg
    44041 caaaaaaata caggtgcatt gcgcatttgt tgccatagtg cttcggggat aaataccgaa
    44101 cctatcaaat gactctcatc ttctatccat atcggtttgc tgaggtcgaa gtttctgata
    44161 ctttcgaaca gtaaattctc aaacatttcg gtgctcggct gaggctcttc tccaagatca
    44221 ccaaaggcac ttcctttgtg atgcgccaaa tgttcaagat ctgcaacctg ctctccggca
    44281 ttgcgcagtg cttgcaaaat ttttgttttt cccgaacccg tccttccacc aagtatatat
    44341 acagtcaact gagatgaaaa taaagcatgt acagctccgc gatatgattt gtagcctccc
    44401 tttaacactt tggtctgaaa ccctgcgaaa tcaaataagg tagccatgct ttgactcctc
    44461 atgccgccac gccaacaatg taccgctatc gcattatctt ttgccaacgc acttgcctca
    44521 tgaataaact tgtcgagttt tggacccgta atagataaac cctttctgat tgcagcttgt
    44581 cttccctcat gtttatataa aatacccacc tgggcacgtt ccgagttgtc aaataaggga
    44641 atgttcaccg ctcccggaat atgacctgtg gcaaactctc ccggactgcg aacatccacc
    44701 aatgggaggc ttcccgcatc cgataaatac gctgatatgg tcatttcctt aaacaaggtg
    44761 tagctttggt gtaaaattat aaaacttgaa catgcttaat gctataggta aaagcttcat
    44821 tgccctgttg ttgatcgctt cactgcatgc gcaggctcaa atgcccatca gtacagaaac
    44881 ataccatgca actctggaca tgcaggggca gcaactcgga tttcaattag aactcgaaat
    44941 tacggctaat tcaagtgaag catatgcccc gttaaatttt actttagtaa atggtgttga
    45001 gcaaattgcc ggtgaagtga tcaaaaggaa cgattctttt gtatgtatca tgccggtgtt
    45061 taatacggaa attgtatttg ttggcgacaa tcatttacaa gaaatccatg gcagatggta
    45121 tgatcattca agacagggaa attattttct tccgtttaag gccacgcaaa tttcacagga
    45181 tgaaaagcgg tacagatatt tcaaacaact tactgccccg aaagcaaata tcagcggaac
    45241 ctttgctgta gcatttaatg atggcgaatc taccgataac actgttggta ttttcgaaca
    45301 gaaaaataat tatctgacag gcacatttct aaccactacc ggcgattatc gttttctgga
    45361 aggggaggtg agtggcgata agttttatct ctctgcattc gatggctcac atgcctttat
    45421 ttttcagggg aaaataattt ccaacgatag tattattggt gtattctata gcggaattca
    45481 ctatctctcc ttattcaatg gtaaacgcga tgctaatgcc aaattacctg atgaaagcaa
    45541 actcaccttt ttaaaacccg gatatgataa aatcagcttc tctttccctg atgaaaatgg
    45601 taaaatggta agccttgatg atgctaaatt ttctaacaaa gctgttattg tcaccatcac
    45661 aggttcatgg tgtccaaatt gtatggatga atcaagttat ttgagtgagg tggaagataa
    45721 atataagaag cagggattag aaattattgc actgagtttc gagcgaaaaa cagatccgga
    45781 aacatttgca tggagcatca ataaagtgcg cacacatttt ggaacagatt tcacatatct
    45841 caatgccggt ttgcccaaaa ctgccgggga tgcattgcca atgctcaata aagtgatggg
    45901 ctttcctacc atgatactgc tcgatcgcaa tcatcaggta gttgccatac atacgggttt
    45961 cagcggaccg gctacaggta aactattcct cgattttcaa acgaaatttg aagaagaact
    46021 aagtagggca ttgaaatgaa acgtatagtg attgaattaa attaatgcac ttgtgtttaa
    46081 ttccgggtgc gaccatattg gttacctgca attagccttg attcgatagc gataaattaa
    46141 atttccccta tatatactaa agcgcagggc aacttcgtta tagcccaaat ccacgaaaat
    46201 gaagtatgca ctttacctga tgctacttat ttcaacgttt tctgcgacag ctcaggttga
    46261 aatcaccaat atctcgttcg agcacaatag tgctgattta tcgtctgatg acctttctaa
    46321 aatcagtgaa attgccggtc ggttgaatgc atcacatgat aatgtgatat ctgttaccat
    46381 ggccgggttt acggatacta caggaacggt tgaatataac gagcaactat catggcaacg
    46441 cacaaaaaat gtagaaaaag ctttgcatga tttgtggccg ggggaagtaa cacttgaatg
    46501 gtatggcgag cgtaacgaag gcaatgccca tgcacaaacg cgttcagtag atattattgt
    46561 aagcatagaa acccttcctc aagttgaaat tcccaaatca aacgccgaaa attttattga
    46621 agtattttca tcagtgcaac agaaggcaca acagttttat ataccaagta atgactcctc
    46681 cataattatt ggtgatcagg gaacagtaat tgatattcct gcaaatagtt ttgatatccc
    46741 taccgcatat gccggtgcta atgtaaccat aacactaaaa gaatattatt tactcagcga
    46801 tatgctgctg gcaggtttta ctacgcaatg tggcgatgat ttattgcagt cgggtggtat
    46861 gatagatgtt aatgcctatg ttgccggaaa aaaggttggg ctgagggatg gaaagaagtt
    46921 acacatcaaa tttaatacca agcgtaaact tggcgaaatg gaattatttg ccggtgaacg
    46981 aaacgaaaaa ggtttggttg tctggaagcg gactaaaaca cctatgactg ccactaacac
    47041 ctatgaattt ataagcctcc tcgcacctgc ttattttaag gaagtttcag acctaaaaag
    47101 ttgtccctac tttttctgtg gtttgcgaca gcaatttgga atgaatacta cgaattcgca
    47161 aaatcgccgg acttatggaa aaattgattg gccggcatac agagcatata tcagcgaaat
    47221 gaaaaagtca tttggcgtga aatatatgac tgaattaagg agggcaatag acgaacacaa
    47281 taagaaagtt ggtgaaataa accaatatgt tgtagaaact tcttccctcg gtttgatgaa
    47341 ttgtgatagg tttatgaatt atcctgtcga acaacgaaca aaggtaattg ccgaatccag
    47401 gcagattgat ggcgcaagtg gctttcttgc cttcacagcc tatcctgcca tgttatctgt
    47461 taattatttc gaagacggaa atattggatt ctcaaatatc ggtcctgata tgaatgccac
    47521 cttagtgctc atcaggaaag atggtgaaaa gtggatgcta tccatccaaa atattgtcac
    47581 tgctcgcgac acccgggtca cacccgcctt cgttgaggtt accccggaag aactaaagac
    47641 caaacttaaa atgttggatg aaaatggata aaacttaagc attccggcac ctattcttcg
    47701 cgccttcgct acttaccgtc tttgcgcgaa actttgttgg aggtttgtta gactgaaatt
    47761 atcagattgc tatcttctta attcatttga tttaggtagc aatctgttta tttatgtata
    47821 gtcttgatgc attgatactc tttccctatc tttaacaggt aaactccaat atgaagcacc
    47881 tttttgctat aactgcgctc ttctttacct catattgtta tacacaaact tctactactt
    47941 cctattacgg tgatgtgtat ttgtcaacga aagcaccaca atcaaaggct aaattttcaa
    48001 aaactactac gctagcagca gatagtacta tcacaacggt ggtaactgat ttaaaaaaca
    48061 accggatcgt gagcagcgaa tccatgaaaa acaatgaagc tgtcggcatt tggttgaagc
    48121 gccatggcgg acaaactgat accctggatt atcaattcga tctgatatat gcggcatcaa
    48181 cttgcaggga aagcttgccc ggactaaaaa atgtttgggc tgataatgac agccttcact
    48241 ataaagcgcc ggtgctcaag gataatgaaa gcgtatatgc agcaatcggt aatgaagtgt
    48301 attatccgca atttgccaaa gaaaatgcca ttgaaggaaa agtatttctt gaattcacaa
    48361 ttacagaaac cggaaaaatt gaaaatatta tcgtggttag aggcgtacat atttctatgg
    48421 ataaggaagc agtgagaacg gttaaaacat taaaattcga atcattgcca atggtaaacg
    48481 gtgttgttac accactctat gttaaaatgc ctttaatgtt taccttggcc gattaaaatt
    48541 aaatttgaat cacatgaaaa ctatattgtt attgcttggc gtttccatcg gtgcaacgct
    48601 tttcgcacag aaaaacccaa cagaacttgc ccaacccata gtagccgaag gtaagttgtt
    48661 gtacaaatcg gaaatggcat catggtttgg caccgatatt ttcttggaga aattctctga
    48721 catgaatatt gtgggaggct atttttctta tactgaaggc gatacctcta catgtatttt
    48781 cttctccgat gcagaaaaac ctgaagtgct tggggaaatt cagtttgaca ctacctttaa
    48841 tccctcaacc gctaaagttg atttggagag acgcagcttc acaaaccttg aaaataatct
    48901 tttcgtgatc cgaagtaaag cgcttgatga attgcgttcg gatactatgt tcatcagata
    48961 taacaataca gattttaatc tgataccggt aattaaggat ggcgaaagga aagtatatgt
    49021 gctgacagga actgcaagta atggggtggt attatttggt aatgactact tactcacttt
    49081 tgatgctgaa aataacttat tgactaagaa gcgaatacat caaaacctta ttcctgtata
    49141 tctcaatggt cccgatgcaa agaatacagt gggcactata cacagccatc ttccggaaac
    49201 gggcgacttt atgaccgcca cagatatctg cacagccatg ctatatcaag atattaccca
    49261 ttgggaaaca attacggttg tgtctgaaaa gtatatgaat atctggaatt gccggacaaa
    49321 taccctgact gtagtcgaaa aagatatctt caaaaaaatc gatgaagatc aggaaaaacg
    49381 ccatggcaat aaggaaaaga ataatgatga ttaagccgca ttcaaagtgc aaatgaatgc
    49441 cgaagaaact tgttacaaat tatgccatta tcatttttct atatggtttt caagataatg
    49501 gcttaagata agaaaaaaaa tgatatgctt aaaaaattag ttgtgacaaa acgtattaaa
    49561 aaaatatttt tccgaacttt tattttaggt tctgtttccg ttattctgct tatacttttt
    49621 tgtaattttc gtatcgaaaa agccggcaag ggaaagttgt atagcgatgt aagtgccatc
    49681 ccacatcagc atgtcggcct gctgcttgga acggggaagt atatgagcga cggttccgta
    49741 aatctgttct acaaatatcg tattgaagcc gcactgtcgc ttttgaaaag taataaaatc
    49801 gattaccttg taatcagcgg tgataatagt cgacccgatt acaacgaacc tgccatgatg
    49861 cgcgatgatt tagttgctgc aggtattgat attagccgta tttacctcga ttacgctggc
    49921 ttccggactt tcgattctgt tgtgcgattg cgtgaaatat tcggaacaga ttcagctaca
    49981 atcatttcac aggaatttca taatgaaaga gctttatata tagccggtaa agaaaatatt
    50041 acggcaatag gattcaatgc gcaagatgtg ggcggaagtg gtggttttaa aacacaactt
    50101 cgtgaatatc tggcaagagt aaaagttttt gtagattaca tcatccacac aaaaccacat
    50161 ttcctggggc caaaagtgca aatacctcaa tgagagcctt gaattaaaat tgcttacaaa
    50221 ccggtattgt agttgtggct ccggtagtgg aattcaatat tcggataaaa taaataccgc
    50281 ttgacaatgt ttcacatggt attgtgatta aattgtcacc catctgcaaa atggtagatt
    50341 tttctgcaac catccttccc attgcgtcta taattgcaat ttgagattgt tgttcgatgg
    50401 agctactcaa ttgcaattgt aagctgttat tttgaaaata ggcatttaca ctagtgtaag
    50461 aaccattttc atgcacgtca accggcatat cacctacaat aaattgatgc tcataatatt
    50521 tccctgccaa ctctgcgctc catgtccaca cgccttccgg aacatcttcc gggatggtgt
    50581 aaaaccaata ccagtaagat gcgctgaaat aatctgcggg ctgataaaag tcccactcat
    50641 accacacact gccgtctgca cgtgtaattt tcaaatggca aaggtctgtc gaggataagt
    50701 ccttagcata caatgcaaaa taacaaacat cgccaacatt aaatgtattc gattcattgg
    50761 tgatttcaac ttccggacaa ggtggaaatg ttggtggtgc aaaatgtgtt tgaagtttat
    50821 tgatttccgg atcgatatat ggtttttgat ctgcccacca gctctcatca ttccattcgt
    50881 tacaagcgcc ggagttgggg tccattaatg caccatctgc atcgtataat tcgaaatgca
    50941 aatgtggacc tgtagaattg cctgagctgc ccacaagtcc taaatattct cctgtttcaa
    51001 caaaatcgcc aagccccttt gccgtaacag acccgttttt catatgacca taccatgccg
    51061 tagagccatc atcatgacga acaaaaactg cattccatga gcccggatta aatgcgcaat
    51121 tctgatcgaa attgccgtcg tatcttgcta caatcattcc cggagctgca gcaacaattt
    51181 gtacttgttc ctcttgcatc attttccatg aaaacggcca taagaaataa tctatgccct
    51241 gatggttgta gcctgcatcg gtgtcatacg ttcttgcacc acaattccaa tccagtaagg
    51301 cacccggaaa cgcagtattg tgatccgcaa aatttgaaat cccatagaaa ttatatccac
    51361 tgaacccggc attccatgca agcggaaatt ctaaagcaag tgcctcggtt ttttgttgtg
    51421 cagggactag tttgccttgt gcacttaaaa cttcaatatt gtgttgcacg tccgaacgca
    51481 tagcagcata ttgctcctga cttatttcat catggggcaa agcagg
//
LOCUS       DAOQOU010000004        48873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_279_length_48873_cov_6.273342, whole genome shotgun sequence.
ACCESSION   DAOQOU010000004 DAOQOU010000000
VERSION     DAOQOU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 48873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48873
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_279_length_48873_cov_6.273342"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLM90_00645"
     CDS             <1..298
                     /locus_tag="PLM90_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HMZ87899.1"
                     /translation="DTLSAEFLQDVRSWIWMPQSLEVSISADGKHYTKLPTIQNTIPG
                     DDYDSKITQVMQVAIPKELVRYIQVTATNHGPVPDWHPGKGGKSWIFCDEIWVK"
     gene            complement(338..1192)
                     /locus_tag="PLM90_00650"
     CDS             complement(338..1192)
                     /locus_tag="PLM90_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ87900.1"
                     /translation="MSISLHMPLSIASLNSGSNANCYYVGNEDDAILVDAGLAYKETI
                     LRMEGLGLDPKRLKAVFISHEHTDHISGVTQLHRKLNLPVYITEKTRLNTALRFRDDL
                     IRPFIANAAIQIGSLHVLPFTKFHDAADPHSFMVYDNEVTIGILTDIGHACEEVINCF
                     KQCDAVFLESNYCEEMLANGSYPAQLKKRISGKSGHLSNRQALELFVKHKSERLQLVL
                     LAHLSQNNNTPEKALSEFRDHIGKIKLHVARRDGASELFEIHPIPAKVYNPPKPTAPK
                     PQQLSLFS"
     gene            complement(1295..>2920)
                     /locus_tag="PLM90_00655"
     CDS             complement(1295..>2920)
                     /locus_tag="PLM90_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ87901.1"
                     /translation="YENMISGYRDGFFFRLDTVGALIWATYAGGFFDEFGRALAVVDS
                     NTIFWGGKTFSYDYPSTFGSYQMAYGGGIADGFFQRVSDCPTLITFYSDDDEDGYGDP
                     GKTINGCFPPDGAVANALDCDDNNPAIHASATEICNGVDDDCNGLTDDADPGITGQPI
                     WYVDADLDSYGNSDVSAIACIAPVGYVAIGGDCNDAQTSVHPGATELCNGIDDDCDAI
                     VDEDVIFTTYYADADNDGFGNALSTTTSCSGIPSGYVVAAGDCNDANGAIYPGATEVC
                     NALDDDCDAVIDEGVASASITPLGATTFCQGGSVILQANTGFGCSYVWKKNGATIAGA
                     NTANYTATKSGNYTVMVTVAGGCTATSATTTVTVNSKPNPVITALGDLNICALGSVTL
                     KVTNKPGDTYQWYKNGVIISGATANTYTATSIGSYHVKETTTAGCTKNATAVSVTSSC
                     KLEAENITSLIVAPNPASDVLYVMFNAGQTLQLFVSLTNTLGQQVYAKATTVESGEQT
                     LTIELNNQLSDGVYYLSLQSENINISKEIILAR"
     assembly_gap    2921..3020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3021..4336)
                     /locus_tag="PLM90_00660"
     CDS             complement(<3021..4336)
                     /locus_tag="PLM90_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87902.1"
                     /translation="MRGTHTILLVLGLLTACSLFAANNSKGNPVFIENKGQITYPDGT
                     PATDVLYSLQTGGFSVFVFRNGLSYQFTAPGHEPNSAQNSEWPFNEEQDETLYVPAKG
                     VHRVDMRFVGSNENAIAYTTPATGYVENYFYGNNIASTQAQSFQKITISEIYPGVDWT
                     VYVSDGKVKYDFIVLSGVDPGIIKMEITGADEVKQKSESEMVYTTRFGEISDGNLFCF
                     QIDEHKPVHSKFVTNGNIISFDVANFDRAKALIIDPALDWSSFYGGFGEDAINGMAVD
                     ADSNVLFTGYTASSNAISYLGYQNIYLGGTYDAYVVKMSPSGTRIWATYFGGKRGDFG
                     TSVAIGANNDIYVAGFAFSTNFITTAGVHQTFNAGDYDGFLIKFNSAGSLLFSTMFGG
                     PMGEFARSVVIDHNNDIYISGSTLSSTGIASGAWQPTLGGSNDEFLA"
     gene            complement(4486..5322)
                     /gene="tsf"
                     /locus_tag="PLM90_00665"
     CDS             complement(4486..5322)
                     /gene="tsf"
                     /locus_tag="PLM90_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMZ87903.1"
                     /translation="MSVTITASDVNKLRQITGAGMMDCKKALSETGGDFDAAIDYLRK
                     KGQKVSEKRADRETNEGVVVATVSSDSAYGTIFGLGCETDFVAKNEDYVNFAKAISTI
                     AMANKPESKEALLALPFESITIAEKIAEQVGKIGEKIEITDYALITGEHVVAYNHSNG
                     KVGVLVAMNKKGDAIEAVGKDVTMQIAAMAPISIDKNDVPEEVKNREMEIGREQARAE
                     GKPEAILDKIAAGKLEAYYKDATLLNQKFVKDNAKSIRDVLTGVEKDLTVTAFKRVAL
                     GA"
     gene            complement(5411..6298)
                     /gene="rpsB"
                     /locus_tag="PLM90_00670"
     CDS             complement(5411..6298)
                     /gene="rpsB"
                     /locus_tag="PLM90_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMZ87904.1"
                     /translation="MEKITYNDLLEAGVHFGHLRKKWNPKMLPYIFMEKKGIHIIDLN
                     KTLEKLDEAAAALKTIARSGKKIMFVATKKQAKEIMEEQARRVGMPFATERWLGGMLT
                     NFTTIRKSIKKLQAIDKMLVDGSAENMTKKERLVLGRERDKLEKVIGGVAQLNRLPAA
                     LLIVDIGHEHIAIAEAHRLNITTFGMVDTNCDPNKVDFAVPANDDAAKSIAIIVKYLT
                     DAIVEGLEERAKSKEEMDEAAREPEFDENGEEIKVNINEEEGRDGRKEGGNRFGKGPR
                     GGGGNKPGGNRRPMAQRKK"
     gene            complement(6317..6703)
                     /gene="rpsI"
                     /locus_tag="PLM90_00675"
     CDS             complement(6317..6703)
                     /gene="rpsI"
                     /locus_tag="PLM90_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMZ87905.1"
                     /translation="MDVINTVGRRKNAIARVFMKKGKGSIIINGKDYKEYISVSHLLH
                     NITDPLETVGMSSEFDFTINASGGGVKGQAEAIKMGIARALVEFNAELKPALRAKGFM
                     TRDSRMVERKKFGLRKARRATQFSKR"
     gene            complement(6709..7149)
                     /gene="rplM"
                     /locus_tag="PLM90_00680"
     CDS             complement(6709..7149)
                     /gene="rplM"
                     /locus_tag="PLM90_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMZ87906.1"
                     /translation="METLSYKTISANPATVQHDWWVIDAENQTLGRLSTKVAAILRGK
                     NKPYFTPHTDCGDFVIIINAEKVRLTGKKMLEKEIDWYSGYPGGRKVANPKTVLANQP
                     HKLLERSIKGMLPHNKLGSAMYKKLFVYAGSEHPHAAQKPKTLK"
     gene            complement(7353..8681)
                     /locus_tag="PLM90_00685"
     CDS             complement(7353..8681)
                     /locus_tag="PLM90_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ87907.1"
                     /translation="MRKLEKPPNINDPELMFEMLAFFRDPASQRLVSQLNNKYIYWDK
                     VRYQPVQEGFTQAHLWAAIKMSRTIEAKVVRFGRKKFFYNLTDTIQRGLHEFDMHMGG
                     NLGSRGLIPEEDKKRYLISSIMEEAIASSQIEGAVTSRKVARQMLLDESKPRNKSEQM
                     ILNNFITIKHIVDIKNEPITIERLLHIHQLISNKTLDNESDEGRFRDHDDIHVVDVTD
                     GEIVFTPPAHGEVEEMMRALIDFFNEDDHTTFIHPIIKGCIIHFIMGFIHPFVDGNGR
                     TARALFYWYLLKKGYWLTEYLSISRLIIRSKQQYAMSFLYCETDENDLTYFLNYKIKM
                     MQLAYAHLQDYIKKQIHEKKQLVDFQRIGGVNNRQAQILLWLYVDSNEIFKVNTIENR
                     FGVSNQTARTDLYGLVELGFMEELTINKKERAFIRSASFLDRMGENSDVL"
     gene            complement(8853..9530)
                     /locus_tag="PLM90_00690"
     CDS             complement(8853..9530)
                     /locus_tag="PLM90_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HMZ87908.1"
                     /translation="MIGAYFIGILFMVAGLIIGGVLRSKFKKYSEIPVNGGLTGKDIA
                     ERMLAENGIYDVQVISVGGQLTDHYNPMNKTINLSEGVYNVCSIAAAAVAAHETGHAV
                     QHARQYSWLQMRSKMVPVVSISSQWLTWVLLAGILLINSYPQILLAGIVMFALTTLFS
                     FITLPVEIDASRRAIAWLQGSSVAGTMEMSKAKDALNWAGYTYVVAALGSLATLLYYV
                     MIYMSRR"
     gene            complement(9662..10228)
                     /locus_tag="PLM90_00695"
     CDS             complement(9662..10228)
                     /locus_tag="PLM90_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMZ87909.1"
                     /translation="MLVPVLIIIGVLALILIGLYNTLVSRKNGVENAFGTIDAMLKKR
                     FDLLPNLVETVKQYMTYEQDTLTRIVELRNSVQSNPGMPVQDRMDAENKLTGLLTQLR
                     VAVENYPDLKANNSFIQLQSTWTEIEEQLSAARRTYNAAVTAYNNSVEMFPTNIMAGM
                     MNYRRKEVLAIPESERQSPSAKDLFNKN"
     gene            complement(10353..11339)
                     /gene="dusB"
                     /locus_tag="PLM90_00700"
     CDS             complement(10353..11339)
                     /gene="dusB"
                     /locus_tag="PLM90_00700"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMZ87910.1"
                     /translation="MPRIGNIELPEFPLLLAPMEDVSDPPFRVLCKEQGADLMYTEFI
                     STEGLIRHAEKSTKKLEFYEAERPIGIQIFGADIDVMKGCAQIVEEAGPDLLDINFGC
                     PVKKVVCKDAGAGILKDIPRMVCLTAEIVKATKLPVTVKTRLGWDDNSKNIMEVAERL
                     QDIGIKALTIHGRTRAQMYKGSADWTLIGAVKNNPRIQIPIFGNGDVDTPEKALEMKN
                     TYGVDGIMIGRAAIGHPWVFREIKHFLNTGEHLAAPTMEERVEVCKKHLLKSVEWKGE
                     VLGILEMRRHYTNYFKGIPNIKSYRNELVNLMDVGALMEVMDTIAQLPEEVM"
     gene            11845..12825
                     /locus_tag="PLM90_00705"
     CDS             11845..12825
                     /locus_tag="PLM90_00705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HMZ87911.1"
                     /translation="MLGSIRHIHWLGQLLILAGVYIITEALFVFLANGIINAMYPGMD
                     TTKLILSLSTIKSADEINDVEASALKWYQAITSLGRFVLVSLFFAELLGEKPGDFLRL
                     KKTPTINGFILIPFIIIAASFSIAVIDDWNAGLHLPSAWSSIEDTMRLWEQQAIIQTD
                     IFLRTETISGLLVNILVICVIAGIGEELLFRGVLQGLIEKGTGKPHLAIWVSATLFSA
                     IHLQFFGFFPRLLLGALNGYLFYYSRSLWAPIWAHFINNLLTVTAIFLTHRGVIQGDI
                     TESSNIWFGLIAAPFVFLLLYLYRNKESHGKRLDAGVLDVRQDGGELSDE"
     gene            12800..12973
                     /locus_tag="PLM90_00710"
     CDS             12800..12973
                     /locus_tag="PLM90_00710"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87912.1"
                     /translation="MEANYLMSELQQHAIYAVLFEKPPMPFGGDVMPVKFELYVHDKQ
                     AEEALELIYNLPE"
     gene            12970..13800
                     /locus_tag="PLM90_00715"
     CDS             12970..13800
                     /locus_tag="PLM90_00715"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HMZ87913.1"
                     /translation="MNNLVLRIITALVGVVIIIGSIFVDRTAFYAVFMLIGFLTHLEY
                     VRTTSATPARGKWIVMGLPLAFGIMIMTLQTGIINGLLYNYIQNDVLIELLLLIIPIT
                     LIVELYLKREFPFQQVGLTVLGLLYVVFPFGLYMNYGAGNFQQAFALGLLCLVWSNDT
                     FAYFAGRLFGKNKLFESISPKKTWEGFIGGLLCTMACGFVLSRFYPEYISATQWIVLG
                     LVVSVAGTFGDLVESMLKRSVQIKDSGSLLPGHGGFLDRFDGFMFAVPAAVFYIDFIR
                     "
     gene            13868..14551
                     /locus_tag="PLM90_00720"
     CDS             13868..14551
                     /locus_tag="PLM90_00720"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HMZ87914.1"
                     /translation="MKFTRFIHKEGRKIIIGFLVFLVILNFCTHYFMPEARLFNTLVL
                     IASLGGIIFVMSFFRNPKRSLFIEEGSIIAPADGKVVVVEETYEGEYFKDRRLQVSIF
                     MSVKNVHVNRNPISGQVKYFKYHPGKYLLAWHPKSSTENERTTFVIEDEDDNVEILMR
                     QIAGTVARRIKFYVDEGDAVTQGSEFGFIKFGSRVDLFLPLDSKVEVKIGEKVKGGQT
                     VLARVMRHN"
     gene            14591..14995
                     /locus_tag="PLM90_00725"
     CDS             14591..14995
                     /locus_tag="PLM90_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87915.1"
                     /translation="MKKLLSFALVTAFGVTVAMAQTATTATTTTTVTATEQVTTAAPV
                     IKAVEAEQGNIKCTGTGSCCSHGVSRSGATTTATSEGAGVSTEVTTTGEAAAVETTGE
                     IKATDAGSCHQLSIGVSAAKPEPAEKTEDPKK"
     gene            complement(15060..15752)
                     /locus_tag="PLM90_00730"
     CDS             complement(15060..15752)
                     /locus_tag="PLM90_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HMZ87916.1"
                     /translation="MQNIHHILFDLDNTLWDFSGNSKRILAQIHNDLSLDQYGIPDFH
                     RFHEQYAFRNEYLWHQYALGNVTRDEVRLNRFYITFNDFGLDNYVLANKAADFYIHHT
                     RKQTDLLPYTRELLDHLHGKYGLHIITNGFDEVQFFKLENTGIRHYFQTVTTAENAQS
                     LKPNKKIFDHALSLIPAKASECLYIGDSPDVDGKGSIDAGMSFIWFNPAKKENKEGYR
                     EVQHLSEIAALL"
     gene            complement(15754..16761)
                     /locus_tag="PLM90_00735"
     CDS             complement(15754..16761)
                     /locus_tag="PLM90_00735"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ87917.1"
                     /translation="MIVVTGANGLVGSYLCRWLLQQGKQVRGMRRADSNMRLVEDIAS
                     QIEWVIGDVTDVVAIENAMSGVDQVYHCAAVISYRKKSAAQMMRINGEGTANMVNIAL
                     DLGIKKFVHISSIAALGRTGKDGEIITEEAPWDMKYITTDYAISKFTAEREVWRAMAE
                     GLKAVIINPSIILGAGDWENGSSKLFSTIYKGFKFYTTGITGYVDVRDVVRIAQVAMD
                     SDITEQRFIVNGDNWTNKDVLQHMARSLHVKEPSVHAGKTLSEIAWRMEWLKGLFSGK
                     EPAVTKQTARIANATTYFNSNKMKDTFHYSCVPLEKTIADTAAAFLAEKTDGKFHPLE
                     F"
     gene            complement(16758..17873)
                     /locus_tag="PLM90_00740"
     CDS             complement(16758..17873)
                     /locus_tag="PLM90_00740"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HMZ87918.1"
                     /translation="MSPERKTYTAIGVMSGSSLDGVDIALCEIKLEAQSSFEIIAAET
                     YKYANQEISYIKSLLPVITDDHHAAHTFFAEIFARHITQFLKLFPDRQIDFIALHGHT
                     LLHFPAQGVSLQLGDPQLMANILGIPVIAHFRQADIDAGGQGAPLVPMCDVIFFKQYD
                     ACLNLGGIANISFESASGRIGFDVCACNQLLNYCAAMAGLDYDPEGELAAAGMIDQDL
                     LDQLNAVPYLDMPWPKSLDNHFVHRAFIDTIAKSTIPLNDKLATAVEHIAIAIAAVII
                     AQPERRPLHQYQLLVTGGGAYNTFLIERIAANAGIKITLPGDKIIQYKEALAMALMGA
                     LRIQSKPNFLQSVTGAKTAVSGGVIAYPEKIIAPLPS"
     gene            complement(17873..18199)
                     /locus_tag="PLM90_00745"
     CDS             complement(17873..18199)
                     /locus_tag="PLM90_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ87919.1"
                     /translation="MKKLLIFFAVAATIASCTTASSSDQDTATAEPTASPGMLVYTKH
                     CKLCHGAKGDLGLSGAANLSITLLSHDEITHVVTEGRKGMPSWKAQLSPQEIQEVTDY
                     VLTLKK"
     gene            complement(18255..20411)
                     /locus_tag="PLM90_00750"
     CDS             complement(18255..20411)
                     /locus_tag="PLM90_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ87920.1"
                     /translation="MKRTRLLLLSLMTIIGLKAHASHIIGGEISYTCIGTNQYQVVLI
                     LYRDCTSFTPFDNPAYMTIYTQDGDYVNNFPLYSPFITGIPAESDNPCLDAPDDVCVE
                     QAIYTAIITVPDGDQGYLLVFQRCCRNAGIVNIETPDDIGATYTQEIPPQTDATCNTS
                     PVFNAFPPTVICIDDPIVFDHSATDIDGDSLAYDFYWPLQGASSFDPAPVIAAPPPYD
                     GVVWVTGYDELYPLDAVPAFAIDPVTGILTGTATAEGRYVVGVRCKEYRDGVWLGDHI
                     RDFQFNVVTCTPTVVAQMDVGDATFDDTTPDDEYLNCSDFTVIFDNYSIGGTEFFWDF
                     GDGSTSTLEDPTHVYADTGTYTVMLIVNPGFVCADTAIGIIKLYNTLTAAYDYSAGCS
                     GTPVTFEDNSNSTEAGDIITWDWNFGDGATSTTANPEHEYADGGTYTVVLTVTTDVGC
                     QSSVSVDILVEPGPDVDFLAPDVCQDEEAEFTNLTTITTGSISSYSWDFGDGATSTED
                     EPSHQYDAPGTYTVTLIAYSANGCSDTLEQDIYIGMLPDAYAGVDDTIMYLETYTLNG
                     TGTGDFVWDPQNPIVPPASISSLLVANPTIELAETTTFVLTVTSPDGCTQTDEVTIYV
                     EDYPIVEVPNAFSPNGDGTNDVISAFLHDISELEQFTIFNRWGQVVYTSDIINDAWDG
                     MIDGKEAEMGTYVYLVVAKDTNGDMITRQGNITLVR"
     gene            complement(20452..22773)
                     /locus_tag="PLM90_00755"
     CDS             complement(20452..22773)
                     /locus_tag="PLM90_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ87921.1"
                     /translation="MILALALCNKVFAFHIIGGEMIYKRLTGNDFEITLKIYRDCSNP
                     EAAPFDDPLQIYVYNASGILVDSLVLFFPGSDNIDPEIVNPCMTVAPDLCVQEAIYVG
                     NINLPPSIGGYDLVYQRCCRNSTIINIYDPIQTGTTYTEHIPDPGTLINSSPEFNNLP
                     PIALCAGYPFAFSHAATDPDGDALVYKFFTPYNGASYDLPDPAPASPPPFNHVIFVPP
                     FNESYQIASSPAFTLGSTTGWLAGTPSDLGQFVVGVAVEEYRGGVLIGTHYRDFQFNV
                     TDCTPDIVASAPPEINNCTDFTVLFDNESIGTDDFYWDFGVPGVTDDNSTEDNPVYTY
                     PDTGTYTVMLIAFPGTDCSDTTFTTVHIYPQLIPGISFENTCAGSPVNFMDISTTDFG
                     TITSWQWNYGDGWGSSEQNPVYSYEEPGNYMLIFAVENSVGCIAEIYDTITIYHLPFA
                     DVATDSACLHTSGTVFDASVVLAGNDIVDWQWEMEPDQYFSGESFDYYFDTAGVYLIN
                     LTVTTDKGCVDSILDTIIVNPQVVAGLLNDTVICEGDSVQVFASGGTYYAWEPPSGVS
                     DPSSPEPWLSPDESTVYGVIVTDGCTSDTAFMQVEILPAPHVIAGPDTIVYHGQEVAM
                     YANGASSFNWEPPDGLSDPFSPNPIALPDVTTLYLVTGTAENGCISTDTALVYIIPNC
                     FNFATVNAFSPNDDGVNDRFRFITAGDDGLVSMEIYNRWGKMIFNTNDIDAGWDGTDG
                     NGHPQELGSYIYVIYTSCDGKEQRLSGSVTLLR"
     gene            23003..25960
                     /locus_tag="PLM90_00760"
     CDS             23003..25960
                     /locus_tag="PLM90_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ87922.1"
                     /translation="MQKILLTALSILGASIIYGQTTFPVNGVYDERSRTYAFTHATIV
                     TDPGNTLQDATLLISDGRIIDVGTAVVIPKNAVVEDLHGKYIYPAFIDLDAGYGMPAK
                     ENPQVGRGPQMLNSNQQAVSWNQAITPEYQAVSGFKPQGDVAKLWRSSGFGLVLTHRH
                     DGIMRGTGALVSLGDERAGEMVVKPNISDHYSFRKGTSTQEYPQSEMGSIALIRQTFY
                     DADWYGRVGKNSEQNISLQAVLDQKNLIHFFDTDEKLEILRVAKIGKEFGYNFIMMGS
                     GNEYQRINEIKQTGAVLVLPLNFPKTPDVSDPWDAELLSISDLKHWEMAPANPAITEQ
                     AGITFALTSSGLDDKSQFLPNVRKALQYGLSEKTALSALTSVPAQILKLESETGSLAK
                     GKRADFFISSDNIFREGAIIYTNWVNGLPYVVNSENSDLRGAYALHIDNMVYGLLIQG
                     KINAPEFSIIKNTDTLKAQGAISTENIQIQFADNGTLYRLTGYHDGKNIEGQGNANDK
                     WINWKAEFASVYAEKPTPQKKDTVQIGTVMYPFTAYGWEAAPTQENILITNATVWTSE
                     KEGVLKNTDVLIGNGKILQVGKNISAKNARIIDGTGKHVTPGIIDEHSHIAVTDGVNE
                     GTQNSSAEVRIADVINCDDINIYRQLSGGVTSAHILHGSANPVGGQTQLIKLRWGAAP
                     EDMKFAQWPGFIKFALGENVKQSNWGDDYSVRFPQTRMGVEQVYVDIFTRASEYAKAK
                     KENPQSVRTDLELEAIAEILEGKRFITCHSYVQSEINMLMHVADSFGFTINTFTHILE
                     GYKVADKMKIHGAGASTFSDWWAYKFEVYEAIPQNPGIMHDAGLVTAINSDDAEMGRR
                     LNQEAAKSVKYFNMSEPEALKMVTINPAKLLHVDDKVGSIKVGKDADIVIWSEDPLSV
                     YAMAEKTIVDGKILYDRSIQDQLMAAEDAERHRIINKMMAEKTNGAITVPIVVTPHRE
                     YDCEDVIDFMKGE"
     gene            25982..27271
                     /locus_tag="PLM90_00765"
     CDS             25982..27271
                     /locus_tag="PLM90_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ87923.1"
                     /translation="MTRYIKQLLTIAFLLHIVATHAQQVPVAISGGRIHVGDGTVIEN
                     GLVIIQNGSIQYAGPMKESGYDANTASLIDATGKDIYPGFIGMGSTIGLNEITAVRST
                     RDAYEVGVYNPNIRSLIAYNTDSKVIPTVRTNGVLLAQVVPYGGVIGGQSSVFKMSGW
                     NWEDAAIATDDNMHMSWPSVYNYDYEKNTYGPNAQYRSEVEDIYTFFAEARSYCNGNA
                     PRERNLRFEAMRKVLNGKQKLFISANSAKQIIGAVALSDSFHIKIVIRGGRQSYRVTE
                     LLKERNIPVLLEATHELPSYDGDPVDLPYKLPAMLHDAGIMCGLSVCNEGDSYWNLRN
                     LPFQAGTAAAYGLNREEALKMITLNNAMLAGIDEKYGTIKTGKSATLFISDGDALDMG
                     TNRLSIMFIAGERLKPDNWQSELSNKYELKYNIQIHR"
     gene            27328..27418
                     /locus_tag="PLM90_00770"
     tRNA            27328..27418
                     /locus_tag="PLM90_00770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27362..27364,aa:Ser,seq:gga)
     assembly_gap    27826..27925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28054..28833)
                     /locus_tag="PLM90_00775"
     CDS             complement(28054..28833)
                     /locus_tag="PLM90_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMZ87924.1"
                     /translation="MPELIDTHTHIYLPEFDSDLDDMIQRALQSGVSHMMMPNIDLES
                     VDAMMALAKKNRACKPMMGLHPCSVKNDADDVMLKIHQLLLDKQYQWYGIGECGLDYY
                     WDLTFVDQQKAAFVTQIRWAKEMQLPLIIHSRNATDDCIDLVSKHKDANLRGIFHCFS
                     GTELQLQQSIDLGFYIGIGGVVTYKNGGLDKVLKPEHLPHLVLETDAPYLSPVPFRGK
                     RNEPSYLVQIVNKLAEILGLPVEHVAEVTSENARRLFGVEG"
     gene            complement(28852..29499)
                     /locus_tag="PLM90_00780"
     CDS             complement(28852..29499)
                     /locus_tag="PLM90_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ87925.1"
                     /translation="MYFVKTPWYLKSVYPSLVWDMPKDNRLFLTFDDGPVPGVTDKVL
                     GILSKYDVKATFFCIGDNVRKHPELYQQILQEGHMTGNHTFHHVNGWKTDTDAYIEEV
                     SACSNLVNSQLFRPPYGRIGYQQIQELKRQYAIIMWDVLSGDFDTQLKPQDCIDHVIQ
                     HAADGSIIVFHDSEKAADRVLPALPVVIEHFLEKGYRFESLPANTASLPRGLHLS"
     gene            29610..31133
                     /gene="gltX"
                     /locus_tag="PLM90_00785"
     CDS             29610..31133
                     /gene="gltX"
                     /locus_tag="PLM90_00785"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMZ87926.1"
                     /translation="MDRPIRLRYAPSPTGAQHLGGIRTALYNYLFVKKLGGKLILRIE
                     DTDQTRYVPGAEEYVINSCAWAGITFDEGVHIGGPFAPYRQSERKDMYRKYAMELIEK
                     GHAYYAFDTPEELEAMRNRMMEQGNPSPAYDHITRQYMKNSVALSKDETERRIQNGDP
                     YVIRIKMPRNEEVKFHDLIRGWVSFNSSQLDDKVLFKSDGMPTYHLANVVDDYLMQVT
                     HVVRGEEWLPSAPTHVMTYRMFGWEEVMPSFAHLPLILKPEGNGKLSKRDGDKYGFPL
                     YSLDWKEQNISGLKEKGFLPEAYINFLAFLGWNPGTDQEVFTEEELIAAFSFERIGKS
                     GARFDPEKAKWFNQQHMRRKTGSELADLLQPYLDKKGIQADRNYLERVCELMKERAVF
                     IPDLYEAGTFFFGEISEYDQDTIRKKWKPEKQEIFLNLIAHIESISNFTEPSIESGVK
                     DYMKENNLGMGEVMPLLRVALAGVLQGPPVFAMMDVLGKNISVQRLHTAIKIFDNFR"
     gene            31149..31652
                     /locus_tag="PLM90_00790"
     CDS             31149..31652
                     /locus_tag="PLM90_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87927.1"
                     /translation="MPKKKPLEGKPEVHKDLDGFEMNINEFGEITSNTDIDKLNQFLN
                     KNVDDKKFRGLDNLPYAKGGDDDEESTEEKDDLNEISDEFEEFRQFNDKDFKDEFDFD
                     EKDEADGYFNEGKHEYEQFEDEEDDAKADDEEDDFDDEEFLDDYADDYGEEDNYNLDE
                     GDFDDKY"
     gene            complement(31718..32425)
                     /locus_tag="PLM90_00795"
     CDS             complement(31718..32425)
                     /locus_tag="PLM90_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ87928.1"
                     /translation="MSSFNFKQFTILQQDSAMKVGVDGVLLGAWAFSKGKVEHILDIG
                     VGTGLISIMMAQRFPEALITGVEIDEAACFEAIENAKRSPWSNRIKIINSDFLQLDEK
                     SSAYDLIISNPPYYEKGWPVEDEGRRKARDAASLPLVDLFQRAAKISGQHTVFCLILP
                     ALALDTCLQTAAINGWFSNRITTVLTKPEKPAKRVLIAFGRSESGTSTDVLTIYDANG
                     NYSEAYKKLTGDFYLAF"
     gene            32496..34070
                     /locus_tag="PLM90_00800"
     CDS             32496..34070
                     /locus_tag="PLM90_00800"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMZ87929.1"
                     /translation="MKNKLLLLTSGILMFSGAFAQLTLTPEPNDTTFIYSLAGPGIVV
                     SNIERNCHTDASAFYNSTDANVGIENGILLSSGTAASSAGPNDVGAASSYLDYPGDTD
                     LEGLTMWLTYDACAISFDMTVMADTVKLKYVFASEEYPEFTFSGFNDIFAFWVSGPGI
                     PTPVNIATVPGTDLPVSINNINATTYSDYYIENGDGYMTPYSTDDYYIQFDGLTTVLE
                     AKLAVTAGETYHMKMAIADASDGIYDSGVFLETGSLGSLRMHTDVFADNDLTFAVEKC
                     ASGHFTFTNEIPSAEPLVIDYMIGGSAINGVDYETIPEQLTIPAWTSEGTIDIVPIHD
                     VIDESFESVILYLYNPQSGVIYDTVTMLIDDEPAAASFASTISDLSVAFTETSGAAVS
                     QYWEFGDGTISTESNPTHIYATAGVYNVCVTGYNAYGCSDTYCDMVTVGNVDINDLQN
                     TLSISPNPVANVLQLEGTENNAVINILNLQGEVVLTSTAYGNSVSIDVSALPAGIYTL
                     RATQGSQMAISNFEKL"
     gene            complement(34132..36666)
                     /locus_tag="PLM90_00805"
     CDS             complement(34132..36666)
                     /locus_tag="PLM90_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87930.1"
                     /translation="MAIVFILQKDDNNFHHTETNPAPVRVDGNHEKEDESYNRDRQNF
                     FDQMHKSAPGTNWRKMDADLRFQLMKQRSNNLRTDDIAMDTLASGALIGEWNEMGSYN
                     TSGRIRATEVDFENETIYAFSDGGNLWKGDLDGENWAVINDNMKITGAHTLRKIGDRL
                     IVGCDQWGVQGVFYTEDEGLTWNATTGLENVAAWGYISDMEILNDANHTIYLLAYEWD
                     YTNWWDIVSLYRSEDLGVSFTKLSSYDVPVYGGANHFVLWSPPYGSPDCYMIENTHVN
                     LLDATGTPVLQGTLPITDDGDYMLCGFEGPSSTSLYVAHRNYVDNFTHFYTSFDGGVN
                     WNTEGIIEEGNFSKNSFNCSQKQEGYLFYGGVDSYRSFTAGNSWVRNNYWYEYYGDIE
                     YNLHADIPFIRTFIDGGGDEIVLISTDGGLYKSTDNGLIWNNITNEGMRNAQYYDVYT
                     YRWVTDILFAGAQDQGYQRSSYTIGDDYYFDQLISGDYGHIVSRSGGDNLWCVYPGFA
                     MYVNDAASASNLTTWDFQGYGHLWMAPIMQDPWNDQVAWWGGGSDAGGAYLWRLQKSG
                     AFISGVKQAKNFSVAGGGSISAISYSPIDPNYWYLLTSGGNFYHSEDAGETWYLSSGF
                     SGPGPQYFYGATIEPSKSQLGVVYIGGSGYDNPAVYMSDDHGDNFVELQDGLPPTLVY
                     DLAVDQTDSLLFAATEVAPYVCVLAEGKWYNIAANDAPLQTYWSVDYVDEINTVRFGT
                     YGRGAWEFAIEKLPTINIHDANQEALMSVYPNPASEKIQITIPAYFSNAEVTVVNMQG
                     DIVYKTKATINKDMPYNIPVSGLAAGSYVIAVKQGEKMYTSKFVRI"
     gene            complement(36749..38371)
                     /locus_tag="PLM90_00810"
     CDS             complement(36749..38371)
                     /locus_tag="PLM90_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ87931.1"
                     /translation="MKRLFSLLILGSVLTMNGQTIPSKPKLVVGIVVDQMAYEFLYRY
                     SDTYSDSGFKRLLRDGYSCENTHYNYIPTYTGPGHASIYTGSVPALNGVVSNDWFNEQ
                     SGAYVYVTDDSTVSMVGGNGLGSKMSPKNMVATTITDALQLSNQQRSKVIGIALKDRG
                     AILPAGHAADAAYWYDGASNAWVTSTYYMQQLPTWVSAFNMQQPAQKMMNKNWDLLLP
                     ASQYNQSTADSVSWETLYTGETSPKFPHYLPFTGNSESIKGTPFGNTLTAQFAMAAIK
                     NEELGTGPETDFLCVSFSSTDYVGHAYGPHSMEIQDTYLRLDKDLGELLTYLDTQVGK
                     GNYLLFLTADHGVTPTPQYMESLRIPAGTTTEVSMLQSMQAMLNAAWGQKDWISAYTN
                     QQIYLNDALLQAAGKTIEDAYNVLYPQLMKMDGVSYVLQINKLNDATMNNDVKELVIN
                     GIYPKRCGDIQILYEPYWFEAYRPTGTTHGSHFSYDTHVPLIFYGWKIKSGALHRNVY
                     VTDIAATLADLLNISEPNACIGHVIPEVANGK"
     gene            38545..>40694
                     /locus_tag="PLM90_00815"
     CDS             38545..>40694
                     /locus_tag="PLM90_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ87932.1"
                     /translation="MARYFLFLTIVCISAALSAQTAETDTSKLKLLPTVNVQAERTIP
                     DIQRIQPVKGTFIYSGKKTEVIDLTQRTVSLSEKYGRQIFSKIPGVFVYDMDGTGNQI
                     NISTRGLDPHRGWEFNIRKDGVLTNSDMYGYPASHYNIPMEAVEKIELVRGTGSLQYG
                     AQFGGMLNYISKQPDSTRALAFETSNTVGSYGLVSTYNGASGKIGKFRYSTWYNKKWI
                     TGYRDNSDAQYDAEAVSLFYDYSKDLTFKFEWTHSNYITHLPGPLTDSMFYADPQMAT
                     RSRNYYNPNIHVPSINMNWNITSSTKLQLTSSAVIGARNSVMFDRPATTEDTIVGATL
                     DYNNRQVDIDHFNSYTNELRLLQSYKLLGHTSNLAAGVQYMYNDLHRQQIGKGTTGSD
                     FDLTLAIPGWGRDLHFITSNIAVFAENRWVLSDRFSVNTGARIEMGESNMSGVITYYP
                     DSTLPNTIKHSFPLFGVSAQYNVSKFTNIYGGWSQAYRPVIFKDIVPASVYEYADKDL
                     KDAYGYNAEVGYRGNWKFLKWDVTAFYLAYNNRLGTLAETDTAGNLIIRRTNIGDSRT
                     TGLEFFMQGDFDLTRKLSLSIFTSTSYMDARYHDATIRSGSENIDIDGNKVESVPDII
                     SRNGLTLKYTRYSLSALYSYVGESYADALNTVEPNASGSVGLVPSYQLIDINIGVKLT
                     NQVKVQFNANNILDEQYFTKRPQFYPGPGIWPSDG"
     assembly_gap    40695..40794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40795..41465)
                     /locus_tag="PLM90_00820"
     CDS             complement(<40795..41465)
                     /locus_tag="PLM90_00820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMZ87933.1"
                     /translation="MFKVNRTVVIVSCFGMFLSGIIAMQSCSEGGGINIFSIEDDKAL
                     GDQIAAEIAGDPVNYPILDSVDYADAYAFVYDIRDRILESGMVFHKDDFKWQVRIIDQ
                     DSVINAFCTPGGYIYVYTGLIRYLENEAQLAGVLGHEMAHADLRHSTDQLTQAYGISI
                     LLDIVLGDDQNAITDIAASLTQLAFSRGDESQADEYSVIYLCPTEYEASGAAGFFEKI
                     EAEGD"
     gene            41571..42542
                     /locus_tag="PLM90_00825"
     CDS             41571..42542
                     /locus_tag="PLM90_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ87934.1"
                     /translation="MADIASIQQPIHEELALFEEKFRASMRSNVALLDRVMYYIVKRK
                     GKQMRPMFVLLSAKMIGNISESSYTAATLIELLHTATLVHDDVVDDAYERRGFFSINA
                     LWKNKIAVLIGDYLLAKGLLVSLDNNEFRMLKITSDAVKEMSEGELLQIEKARRLDIK
                     EDVYFEIIRKKTATLIASACSAGAASVTTDEDTISLMRLFGEKIGIAFQIKDDLLDYG
                     TDDIGKPLGIDIKERKLTLPIIYTLLHAPKNDKSEIMRIIKHYNTDKEKVNWVIDYVK
                     KGGGIAYATEKMKQYQQEAFELLEKFPESESKRALKDLVIFTTERKK"
     gene            complement(42552..46547)
                     /locus_tag="PLM90_00830"
     CDS             complement(42552..46547)
                     /locus_tag="PLM90_00830"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ87935.1"
                     /translation="MFTRTILLIGLLGSFCFAKAQLVINEIATNGAVLDAYNEESDWL
                     ELYNNSATAIDLNGYALTDDPLFPNKWPLPAIMLDANEHLLLLANGNNTYALIDHWES
                     AVLNETERTYFVGTSEPPSDWNTVGFDDTGWSTGPGGIGFGDGDDNTVIASATSCYMR
                     CAFDVADVSVIENAKLHMDYDDAFVAYLNGVEIARSGNIIGTPPTYNTLANADHEAGM
                     YAGYPAEEWNIPAVVMNILLVPGENILSIQVHNVTAGSSDLSSNAWFSVGINNGLTYF
                     SPVPAWFAEPVVYSETNFKITNTGETIYLFDNTGAIADMVNPGSVTYGHAYARIPDGA
                     ASWCTTTTPTPDNSNDGAPCFAGYEPLPVFSLAAGFYTGTQTLTLSSTSPTAQIRYTT
                     DGSLVTATSPVYTGPITISSNEVVAARCYSTGTLLTGEQVKRSYFIDEDDYTLPILSI
                     SIDPGSLFDYDTGIYEFGCCYDAAYPYFGANFWQPWERFGHIEYFTADGDFEWGKDMS
                     LEIHGGWSRAEAQRGFRVDFKNKYGGNLEYPLFGGKPDASSINNFNIRSGGQHVWTYK
                     IQDAFLAKVMEDTHIDYEEWQPCMLFINGDAWGLYEIREKADEHFVESNYGINNNDVD
                     LLNSYSVLNGSDTGWWNIYSSLMSLDPLSDIFYDKFNQAFDMQNYIDYYIGEIYYQNV
                     DFGGYYWGANNMKVWRNRYGGKWRFIMYDMDGAMGYFGSSPYDNYINLTRSPAYPSSY
                     SQIFDRVLDNQEIREYFVNRFADLINTIYQPDNMEAVLYEMRDSILSEIPHQVERWGA
                     PNTATVNSYVDGLLSYNNSRKNAARTHINNSFDLDGQRTTTLAVSPAGAGYIKINTIV
                     PTELPWTGIYFDGVPITITAVPNPGYTFDSWLPNALLPGGSTDISLTINMDNSTTFTA
                     VFTGTAVTPEIIVSEINYNSYVDYNMGDWIELHNAGTDAIDISYWKITDRYEGNAYIL
                     PAGSVIESDEYLVIAKDPIAFSNVHPDITNVVGGYDFEWDNSGDDIRLFDIAGNEIIH
                     MAYADSLGWPQGADGTGRTLELINPTADMNNPGSWFDGCIAGSPGEAYSPCNDALVFS
                     EINYNSSAAMNAGDWVEILNTTADDMDISGWKFLDGEDSLIYTFESGTTIPANDRLVL
                     SGDLDLFESRHPDITNVTGAFNFGLDASGEEIRLFNDHGILQFTVIYDDAAPWPYEAD
                     GAGKTLELESASGIMNNGDNWFAGCPEGSPGTIYDPDCDGVIAIEESVPASLLVFPNP
                     AKNYVMIAFENNYNSGRIELCNLSGQIIYETQSLENGQVIIPRNEIASGVYRVRLIAD
                     KKMIESLLVWE"
     gene            46672..48003
                     /locus_tag="PLM90_00835"
     CDS             46672..48003
                     /locus_tag="PLM90_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ87936.1"
                     /translation="MSFPPGFIANLQHNIGFDKAAFVASHDEVAPVSIRYNPLKSAKR
                     LSDDPVPWCENACYLAERPLFVYDPLFHAGAYYVQEASSMFLDFILRKLDADKKPLRI
                     LDLCAAPGGKSTLIAGAMHEESILFSNEVIKTRAPILRENLTKWGKENVVVLSQDPAT
                     FTGMQEYFDIIVVDAPCSGSGLFRKEPAAIAEWSEENVITCSKRQQRILTDILPALKP
                     GGFLIYATCSYSMEEDEHVVSHFIEQGLTNRNDIISSIETQGIISTALGYRFYPDKIR
                     GEGFFISILQKNNAEELAISKGKQLNYQLASSEEREIIRPWLKNFDKFIFVKHNNVFF
                     AWTKQLQEALKAASGLNILQAGIAIGEIKQGSLIPHHALAMSNIISDNIPRIALSKSE
                     ALEYLRKNNIELNSSEPGWHIITYENIALGWVKMIPGRHNNYYPTEWRLRK"
     gene            complement(48019..48477)
                     /locus_tag="PLM90_00840"
     CDS             complement(48019..48477)
                     /locus_tag="PLM90_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ87937.1"
                     /translation="MKNTLRFPIAALAFLVLPLIATAQNGTPEAGRQDSLPVMDTTDH
                     TPNMIYEYLETMPEFPGGTMALYEFINTNLQYPEAAKKKKIEGNVYAKFVVTEDGSII
                     NIAILRDIGGGCGEEVVRILSTMPKWKPGMQNGKPAAVFYKLPIKFTLSD"
     gene            48563..>48873
                     /locus_tag="PLM90_00845"
     CDS             48563..>48873
                     /locus_tag="PLM90_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87938.1"
                     /translation="MKKIYSLMLGVVAWGAVLAQPVTIGTTTYDLQTNSANKSRLHVY
                     DDGKVSALWTGSTSLAATFSDRGMFFNKYNGTTWGGFPTARIEGVRTGFGEIINVGDH
                     E"
BASE COUNT        14343 a        10281 c        10025 g        13924 t
ORIGIN      
        1 agatacattg agtgccgaat ttctacagga tgtgcgcagc tggatttgga tgccccaatc
       61 attggaagta agcatctctg ctgatggcaa acactacacc aaattgccca ctatacaaaa
      121 cacgattccg ggagatgatt acgatagcaa aataacgcag gttatgcagg ttgccatacc
      181 gaaagaatta gtacggtata tacaagttac tgcaacaaat cacggtcccg ttcctgattg
      241 gcatccgggg aaaggaggaa agagttggat attctgcgat gaaatttggg tgaagtaatt
      301 tcaatgcaca ccatcttgta ataattcacc atttgcatta tgaaaacaaa ctcaattgct
      361 gtggcttcgg cgctgtgggt tttggcggat tatacacttt agccggtata ggatgaatct
      421 caaacaactc agaagcacca tcgcgtcggg caacgtgtaa tttaattttt ccgatatggt
      481 ctcgaaattc agataacgcc ttttccggtg tattgttgtt ttgagaaagg tgtgcaagca
      541 aaactaattg caatcgctca gatttatgtt ttacaaataa ttccaatgct tgcctgttgc
      601 ttaagtgtcc ggacttcccg ctaattctct ttttcaattg agccggataa gatccgttag
      661 caagcatttc ttcacaataa ttcgactcga gaaaaacagc atcgcattgc ttgaagcaat
      721 taattacctc ctcgcaagca tggccaatat ctgttagtat gccaatggtg acttcgttgt
      781 catacaccat aaaactatgt ggatctgccg catcatgaaa tttggtgaag ggaagtacat
      841 gcagcgagcc gatttgaata gccgcattag ctatgaatgg acgaatcaga tcatctctaa
      901 aacggagggc agtattcagg cgtgtttttt ctgttatata aacagggaga ttcagcttgc
      961 ggtgtaattg cgttacgcca ctgatatgat ccgtatgttc atgtgaaata aatactgctt
     1021 tcaaacgctt tggatcaagt ccgagacctt ccattcgaag gattgtctct ttgtaggcta
     1081 atcccgcatc aaccaaaata gcatcatctt cattccccac ataatagcaa ttggcattag
     1141 agcctgaatt aagtgatgct atggaaagag gcatatgtaa agatatactc aaaaaataaa
     1201 attgagtgaa ccaaaactgt taaagcaaca aaaaaccgca atgttgaaaa aaacaacact
     1261 gcggcttaca aatcatttta gcgatagtat caacttatcg cgccaggatg atttcctttg
     1321 aaatatttat attttctgat tgcagcgaca ggtaatatac accgtcggac aattgattat
     1381 taagttcgat ggtcagcgtt tgttctcccg actcgaccgt agttgcctta gcataaactt
     1441 gttgccctaa ggtgttggtt aatgaaacga acaattgtaa ggtttgcccg gcattaaaca
     1501 tcacatataa tacatcgctt gcaggattag gtgcaacgat cagcgaagta atattttcag
     1561 cttcgagttt acatgaagag gtaacactaa ctgccgttgc atttttcgta caaccggcgg
     1621 tagtagtttc tttcacgtgg tatgaaccga tagatgttgc agtataggta ttagcggttg
     1681 caccagaaat gattacacca tttttatacc attggtaagt atcgccgggt ttattggtta
     1741 ctttaagtgt gactgaaccg agtgcgcata tattcaaatc acccagcgct gtaatgacag
     1801 gatttggttt actgttaacg gtaacagttg tagtagcgga agtagcggtg cagcctcctg
     1861 caaccgttac catcactgta taatttccgg atttggtggc tgtataattt gcggtattag
     1921 cacctgcaat agtggcgcca tttttcttcc acacgtatga acagccaaag ccggtatttg
     1981 cttgcaaaat cacactccca ccttggcaaa atgtggtagc acctaaaggc gtgatgcttg
     2041 ccgaagcaac accttcatca attacggcat cacaatcatc atctaatgca ttacaaactt
     2101 ctgtagcacc cggataaatt gctccgttag catcattgca atcgccggcg gctactacat
     2161 agcccgatgg aataccgctg catgaagttg tggtagataa tgcatttcca aaaccatcgt
     2221 tatcagcatc tgcataatac gtagtaaaaa ttacatcctc atcaactatt gcatcacaat
     2281 cgtcgtcaat tccattgcat agctcggttg caccgggatg aacagatgtt tgagcgtcat
     2341 tacaatcgcc gccaattgca acatatccta caggagcaat acaagctatc gctgaaacgt
     2401 cgctatttcc gtaggaatca agatctgcat caacatacca gataggttgt cctgtaattc
     2461 cgggatcagc atcatcagta agtccattgc aatcatcgtc tacaccatta catatttctg
     2521 ttgctgatgc gtgaatggcc ggattattat catcacaatc caaagcattc gctacggctc
     2581 catcgggagg gaagcagccg tttattgttt tacccggatc gccatagcca tcttcatcat
     2641 catcagaata gaaggtaatt aatgtcggac aatcgcttac acgttgaaaa aatccatcgg
     2701 ctattccacc gccatatgcc atttggtaag agccgaatgt ggatgggtaa tcgtagctga
     2761 atgttttccc gccccaaaaa atagtattgg aatcaacaac ggccaatgct cttccaaatt
     2821 catcgaaaaa gccaccggca taggttgccc aaatcaatgc acctacagta tctaaacgaa
     2881 agaaaaatcc atcgcgatag ccggaaatca tattttcata nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn gcaaggaact catcgttact tccccccaat gtcggttgcc
     3061 atgcacctga agcaattcct gtactgctca atgtgcttcc tgatatatat atatcattat
     3121 tatgatcaat aacaacagat ctggcgaatt cacccatagg cccgccaaac attgtcgaga
     3181 acaaaaggct gccggcagaa ttgaatttaa ttaaaaaccc atcataatcg cctgcattaa
     3241 aagtttgatg gacgccggca gttgttatga aattagtgct gaatgcaaat cctgctacat
     3301 aaatatcatt attcgcacca atagctactg atgtgccgaa atcacctcgc ttaccaccaa
     3361 aatacgttgc ccaaatgcgc gttcctgaag gcgacatttt aacaacataa gcatcataag
     3421 ttccgccgag ataaatattt tgatatccga gataagatat ggcattagag cttgcagtat
     3481 atcctgtgaa aaggacattg ctatctgcat caactgccat tccatttatc gcatcttcac
     3541 caaagccacc ataaaaagat gaccagtcca acgccgggtc aatgattaat gcttttgcgc
     3601 ggtcgaaatt tgcgacatcg aaacttataa tattaccgtt ggttacaaat tttgaatgga
     3661 caggtttatg ttcatcaatt tgaaagcaaa acaaattccc atcgctaatc tctccaaatc
     3721 tggtagtgta aaccatttca gattcagatt tttgtttcac ctcatcagca ccggtaattt
     3781 ccatttttat gattcccgga tcaacaccgg acaacacaat aaaatcgtat ttcacctttc
     3841 catcgctcac atagacagtc cagtcgactc ccggataaat ttcacttatc gtaatttttt
     3901 gaaatgattg cgcctgagta ctcgctatgt tgttaccgta aaaataattc tctacatatc
     3961 cggtcgccgg tgtggtatat gctatggcat tttcatttga tccaacaaag cgcatgtcaa
     4021 cacgatgaac accttttgca gggacatata acgtttcatc ctgttcttca ttgaacggcc
     4081 attcggaatt ttgtgctgaa ttcggttcat gacccggagc agtaaattgg tagctcaggc
     4141 cattgcgaaa tacgaataca gagaatcccc ctgtttgcaa tgagtacaat acatcggtag
     4201 ccggtgtgcc gtccggataa gtaatttgtc ctttgttctc tataaacacg ggattgccct
     4261 tactattatt tgcagcgaat agactgcaag cggtaagcaa ccccagtaca agaaggattg
     4321 tgtgtgtgcc tctcatgatt ggtcgcctgt ggaagtataa agttacgatt attaaataga
     4381 tgaaacgcca ccaataaagg ttgtatgcat cttttgtatg atgttttata caaaaaaggc
     4441 tgccggttga ggggcagcct ttctgaattt gtacgtaatc gaatattatg cgcctaaagc
     4501 cacgcgtttg aatgcagtaa ctgtgagatc tttctcaaca cctgttaata catcgcggat
     4561 agatttagcg ttgtctttca caaatttctg gttaagaagt gtagcatctt tgtaatatgc
     4621 ttccaatttt ccggcagcaa tcttgtcgag gatggcttcc ggttttcctt cggcacgagc
     4681 ttgttcgcga ccgatttcca tttcacgatt tttcacctct tcaggaacat cgtttttatc
     4741 gatagagata ggagccatag ctgcaatctg catagtaaca tctttaccaa ctgcttcgat
     4801 tgcatcgcct tttttattca tggcaacgag tacaccaact ttaccattgc tgtggttata
     4861 agcaacaacg tgttcgccgg taatcaaagc gtaatcagtg atttcaattt tttcgccaat
     4921 ttttccaact tgttcagcga ttttttcagc gatggtaatg ctttcgaaag gcaaagcgag
     4981 taatgcttct ttgctttcag gtttattagc cattgcgatt gtggaaattg cttttgcaaa
     5041 attcacatag tcttcatttt ttgcaacgaa gtccgtttcg caacccaaac caaaaatagt
     5101 tccataagcg ctatcgcttg aaacagtggc aactacaaca ccttcattcg tttcacgatc
     5161 ggcgcgtttt tcacttactt tctgaccttt tttgcggagg tagtcgatag cggcatcgaa
     5221 atcgccaccg gtttcgctca gtgctttttt acaatccatc attccggcgc cggtaatctg
     5281 acgcagtttg tttacatcac ttgcagttat tgttactgac atattattga gtgttttttt
     5341 atttaaaaaa cccttccgcc tgagggagga agggttttgt ttaatgtgat attaaaaccc
     5401 taacgggcga ttatttcttg cgttgagcca ttgggcgacg gtttcctccc ggtttgttac
     5461 caccgccacc gcgaggtcct ttaccgaaac ggtttccacc ttctttgcga ccatcacgac
     5521 cttcttcttc gttgatattt acttttatct cttcgccatt ctcatcaaat tccggttcgc
     5581 gtgcagcctc atccatttct tctttgctct tagcgcgttc ttcgagacct tcaacaatag
     5641 catctgtaag atacttcacg atgatagcga tagatttcgc tgcgtcgtcg tttgcaggaa
     5701 ctgcgaagtc aactttattc ggatcgcagt ttgtatctac cattccgaat gtagtgatat
     5761 tgaggcgatg cgcttctgcg attgcaatat gttcgtgacc gatatcaacg atcaacaatg
     5821 ctgcagggag acgattcaac tgagcaacac caccgattac tttttcaagt ttatcgcgtt
     5881 cgcgaccgag tacgaggcgt tcttttttgg tcatgttctc tgcagaaccg tcaaccaaca
     5941 ttttatcgat agcctgtaat tttttgatgc ttttgcgaat agtggtgaag ttggtaagca
     6001 taccaccaag ccaacgttcg gtagcgaaag gcatacctac gcgacgagct tgttcttcca
     6061 tgatttcttt cgcttgcttt tttgtagcaa cgaacataat ctttttacca ctgcgcgcga
     6121 tagttttcaa tgcagcagca gcttcatcga gtttttcgag cgttttattg aggtcgatga
     6181 tgtgaatacc ttttttctcc atgaagatat aaggcaacat cttcgggttc catttcttac
     6241 gcaggtggcc aaagtgtacg cctgcttcca gaagatcgtt atatgtaatt ttttccatct
     6301 tactgtactt gatcgattaa cgtttagaga attgcgttgc acggcgtgct ttgcgaagac
     6361 cgaatttctt gcgttcaacc atacggctgt cgcgtgtcat gaatccttta gcacggagtg
     6421 ccggtttcaa ttcagcgttg aattcaacga gtgcgcgggc gatacccatt ttgatagctt
     6481 ccgcctgacc tttaacacca ccgcctgaag cattgatggt aaagtcgaat tctgagctca
     6541 tgccaactgt ttccagtgga tcggtgatat tatgcaaaag gtgggaaaca gagatatact
     6601 ctttgtagtc tttaccattg atgataatgc taccttttcc tttcttcata aatacgcgag
     6661 caatggcgtt tttccttctg ccaacggtat taataacgtc catgtagatt attttaacgt
     6721 tttaggtttt tgtgcagcat gcgggtgttc agaacctgca tacacgaaaa gtttcttata
     6781 catggcagaa cccaatttgt tatggggcaa catgcctttg atagagcgtt cgagcagttt
     6841 gtgtggctga tttgccaata cagttttagg attggcaacc ttgcgtccac ccggataacc
     6901 ggaataccag tcgatttctt tctctaacat ctttttaccg gtaaggcgca ccttttcggc
     6961 attgatgata attacgaaat ccccacaatc tgtatgaggg gtgaaatacg gcttgttttt
     7021 gccacgcaaa atggcagcca ccttggtgct cagacggccc agggtttggt tttcggcatc
     7081 gatcacccac cagtcgtgct gaacggtggc aggattcgcc gagatcgttt tatagcttaa
     7141 cgtttccact tgttttgttt taaaaattgc tatcccggtc ttaaacggga cggcaaagat
     7201 agatttaccc ctcagaaaaa ccaaggaata tcaacgaatt ttcctacacc cctttcgtaa
     7261 gtaattgatt ttcagcgaaa ttttggcgta ctaatttaat taagtcacct tatttcatgc
     7321 agtttagttt gcatgaaaaa gtagtaatca aactacaata catccgaatt ttcgcccatt
     7381 ctgtcgagga agcttgcaga tcttataaat gcacgttcct ttttattgat tgtcagctct
     7441 tccataaagc ccaattctac taggccatac agatctgttc tcgcggtttg attagacacg
     7501 ccaaatctgt tttcaatggt attaacctta aaaatttcat tggaatcgac atacagccac
     7561 agcaatattt gagcctgccg gttgttcaca ccgccaatgc gttgaaaatc gaccagttgt
     7621 ttcttttcat ggatctgctt cttaatataa tcctgaagat gtgcataggc taattgcatc
     7681 attttgattt tataattcag gaaataagta agatcatttt catccgtctc acaatacaaa
     7741 aacgacatgg catattgctg tttactcctg ataatgagcc tggaaattga caaatactca
     7801 gttagccaat atcctttctt taacaaatac caataaaaca aagctctagc ggtgcgccca
     7861 tttccgtcga caaatggatg aatgaatccc attatgaaat gtatgataca tcctttaatg
     7921 ataggatgaa taaaagttgt atgatcatct tcattaaaga aatcgattaa tgcgcgcatc
     7981 atttcttcca cctcaccatg agcaggaggc gtgaatacaa tttcgccatc ggtaacatcc
     8041 actacatgta tatcatcatg atctctaaac cgcccttcat cactttcatt gtcaagggtt
     8101 ttgttggaaa ttaattgatg tatatgcagc agtcgctcta ttgtaatggg ctcgtttttg
     8161 atatcaacga tgtgctttat agtaataaaa ttgttgagta tcatttgctc cgacttattc
     8221 cgaggcttgc tttcatccag cagcatttgc cgtgctactt ttctggatgt aacagcacct
     8281 tctatctgac tggatgcaat tgcttcctcc ataatagaac ttatcagata tctttttttg
     8341 tcctcctccg gaatcaaacc ccgactcccc aggttcccac ccatatgcat gtcaaattca
     8401 tgtaaacccc tttggatggt atccgtgaga ttatagaaaa acttctttct gccaaaacga
     8461 acaacttttg cttctatcgt tctcgacatt ttgatggcag cccataaatg cgcttgcgtg
     8521 aaaccttcct gaacaggttg ataccgcacc ttgtcccagt agatatattt attgttcaac
     8581 tggcttacca accgctgtga agccggatcc ctgaaaaacg ccaacatttc aaacattaat
     8641 tcaggatcat taatgttagg cggcttttct aattttctca tattatttcc taatttatca
     8701 aatatttgga caaattacga aattagcaac ttgtataaat atttccaaat tttacacaaa
     8761 ttaggaaata ttggtaaatc gagttggaag tgtaataaaa aaggagaggc aaaacctctc
     8821 cttatcaata ttttaaagct tttggccgcc gattaccttc tgctcatgta gatcatgaca
     8881 tagtacaaca gggtagccaa tgaacccagg gcagctacaa cgtaagtgta accggcccag
     8941 ttcagggcat cttttgcttt cgacatctcc atggttccgg caactgaaga gccttgtaac
     9001 caggcaattg ccctgcgact ggcatcaatt tctaccggaa gggtgataaa gctgaacaag
     9061 gttgtaagcg caaacattac gattcctgct aataggattt gcgggtatga gttgatgagc
     9121 aggatacctg ccaacaacac ccatgtaagc cattgcgatg aaatactcac cacaggcacc
     9181 attttactgc gcatttgcaa ccatgagtac tgacgggcat gttgaacagc atgtccggtt
     9241 tcatgggccg caacagctgc agcagctata ctacacacat tgtaaacgcc ttcgctgagg
     9301 ttgatggttt tattcattgg attgtagtga tcggtaagtt gcccgccaac gctgattacc
     9361 tgaacatcat aaatgccatt ttcagcaagc atacgctcgg cgatgtcctt gccggtgaga
     9421 ccgccattta cagggatctc tgaatatttt ttgaacttac tcctcaatac tccgccgata
     9481 atcaaaccgg caaccatgaa taaaatacct atgaaatatg ctcctatcat ttggtttgtt
     9541 ttttattgtt tactgacgtt tgtaacgcaa agatattgca gtttgttcag ttggaaacca
     9601 ggcaagtaaa tacaccttta cgttacacta aatgagaacg tatttgcggc gataaagttt
     9661 cttaattttt attaaataaa tcctttgcac ttggcgattg acgctcactt tcgggaattg
     9721 cgagcacttc tttgcggcga tagttcatca ttcctgccat gatattggtt gggaacatct
     9781 ccacgctatt attatacgcg gtaactgctg cattgtaagt tctgcgggct gccgagagct
     9841 gctcttcaat ttctgtccaa gtgctttgga gttgaataaa gctgttattc gctttcaaat
     9901 cgggatagtt ttcaacggca acacgcaatt gggtaagcaa tcccgtaagc ttattttctg
     9961 catccatacg atcctgaaca ggcatgcccg gattggattg tacgctgttg cggagttcta
    10021 cgatacgggt gagggtatcc tgttcatagg tcatgtattg cttcactgtt tcaaccagat
    10081 tcggcagcag atcgaaacgt ttcttcagca tggcatcaat ggttccgaag gcgttctcga
    10141 caccattttt acgacttaca agtgtgttat aaagtccgat caggattaag gctaaaacgc
    10201 ctataataat taataccggt actagcatgg ttgtgtgatt ttaatgacca aaattaaata
    10261 aaatgcaata ggatgaggta taatttttag ctgaagcaat caaaacggca taaataccgg
    10321 cacttaagga aataatttgt tgccatgccg atttacatta cctcttccgg cagttgagca
    10381 atggtatcca ttacttccat cagcgctcca acatccatta agtttaccag ctcgttgcga
    10441 taggatttga tattcggtat gcctttgaag tagttggtat agtgtcgacg catttccaga
    10501 atgccgagca cttcaccttt ccattctacc gactttagca gatgtttctt acatacctct
    10561 acgcgttcct ccatggtagg cgccgcaagg tgttcgccgg tatttaagaa gtgcttaatc
    10621 tcacgaaaca cccatggatg cccgatcgca gctctgccaa tcatgatgcc atcgacacca
    10681 taagtatttt tcatttccag tgccttttcc ggtgtatcta catcaccatt gccgaaaatg
    10741 gggatctgaa tacgtggatt attctttaca gcacctatca atgtccaatc ggcagaacct
    10801 ttatacatct gggcacgtgt acgaccatgg atggtcaggg ctttgatgcc gatatcctgt
    10861 aagcgttcag caacttccat gatgttcttg gagttatcat cccatccaag tctggttttt
    10921 acggtaacgg ggagcttggt agccttcacg atctcggcgg tgaggcaaac catacgcgga
    10981 atatccttga ggataccggc accggcatct ttgcacacta cctttttcac cggacaacca
    11041 aaattgatgt cgagcaggtc gggtccggct tcttcaacga tctgggcgca acctttcatt
    11101 acatcgatat cggcgccaaa gatctgaatg cctataggtc gttcggcttc gtagaattct
    11161 aattttttgg tgcttttctc agcatgacgg atgagccctt cagtagagat gaactcagtg
    11221 tacatcaagt cggcaccttg ttctttgcaa aggacacgga atggcggatc actcacgtcc
    11281 tccataggag caagcaacaa cggaaattcc ggcaactcaa tatttccgat cctcggcatg
    11341 atgcaaaaat acggcgtttt gggtgagtgg gtcagtgggt gagtgggtga gtgggtgagt
    11401 ttaactcggt gagtgggctg gttttggaat tggcattagg gctaacaaat acgcacttag
    11461 cgccttatca actttgcgac ttagcgcgaa cagagtgact taaggctagc cacgatctcc
    11521 tccccatggc gagtatctgg aattggtatc aggtatcaag tatcaggtat caggtatcaa
    11581 gtatcaagta tcaggtatca ggtaaaaaaa attcacgact cacgattcac gcttcacgat
    11641 tggtagtgag tagtgagact gaaggtattg ttcatttaac tgtgttatta tttgagacta
    11701 atacaattat tcgctcgctt tgcgagcgaa tttgcactca ccactcacca ctcaccactc
    11761 acccactcac ccactcacct cctctctcct ctctcctctc tcctctctcc tttttcctct
    11821 ttcctctttc cttacctttg ttaaatgttg ggaagtataa ggcatatcca ttggcttggt
    11881 cagctgctga ttttggccgg agtatatatt attaccgagg ccctatttgt atttctcgca
    11941 aacggtatca tcaacgccat gtatcccgga atggatacta caaaattgat attgtcgctc
    12001 agcacgatta aatcggccga tgagataaat gatgtggaag cttctgcatt aaaatggtat
    12061 caggcaatta cctctttggg aagatttgtt ttggtgtctt tatttttcgc tgaattactt
    12121 ggtgaaaagc cgggtgattt tttgaggtta aaaaaaacac cgaccatcaa tggatttatt
    12181 ttaattccct tcatcatcat tgctgcatca ttttcaattg ccgtaatcga cgactggaat
    12241 gcagggttgc atttgccgtc ggcatggagc agcatagaag acaccatgcg attgtgggag
    12301 caacaggcga tcatacaaac agatattttc cttcgcacag aaaccatttc cggtttattg
    12361 gtaaacatac ttgtgatttg cgtaattgca ggaataggag aagaattatt attcagaggt
    12421 gtgttacagg gactaattga aaaaggaacc gggaaaccac atttggcgat ttgggtatct
    12481 gcaacattat tcagtgccat tcacttacaa tttttcggat tttttccgcg tttacttttg
    12541 ggagccctga atggttactt attttattac agcaggagtt tatgggcacc gatttgggca
    12601 catttcatca ataatctgct aacggttacg gcaatttttc tgacacatcg cggagtaata
    12661 caaggagata tcaccgaatc aagtaatatt tggtttggat tgattgcagc accttttgta
    12721 tttttattgc tgtacctata tcgcaacaag gaatcgcatg gaaaaagact ggatgcaggt
    12781 gtactcgacg tacgacagga tggaggcgaa ctatctgatg agtgaattgc agcagcatgc
    12841 catctatgcc gtattatttg aaaaaccacc catgcctttt ggtggtgatg tgatgccggt
    12901 gaaattcgaa ttatacgtac acgataaaca agcagaggaa gctctggaat taatttataa
    12961 tttacctgaa tgaacaatct cgttttacgc attataactg ctcttgtggg agtagtcatt
    13021 attattggaa gcatttttgt tgatcgaaca gccttttatg ccgtttttat gctgataggt
    13081 ttcctgacac atctggaata tgtgagaact acttcagcta ctcctgctcg cggcaagtgg
    13141 attgtgatgg gactaccgct tgcctttggg ataatgataa tgacattgca aacaggaata
    13201 ataaatgggc tactatataa ttatattcaa aatgatgttt tgattgagct cctactccta
    13261 atcataccga ttacgctaat agttgaatta tatctcaaac gcgaatttcc ttttcagcag
    13321 gtcggactaa ctgtattagg attgttatat gtggtatttc cttttggcct gtacatgaat
    13381 tatggagccg gtaactttca gcaagctttt gcacttggtt tgttgtgctt agtgtggagc
    13441 aacgatactt ttgcttactt cgcgggaaga cttttcggta aaaacaagtt gttcgagtcc
    13501 atttcaccaa aaaaaacctg ggaaggcttt attggtggat tgctatgcac catggcatgt
    13561 gggtttgtgc tcagcagatt ttacccggaa tacatcagtg caacacaatg gatagtgcta
    13621 ggcctggtag tttcggtagc cggcactttt ggcgatctgg ttgagtcgat gttaaaacga
    13681 agcgttcaaa tcaaggattc cgggagtctg ttgcccggtc atggcggatt tctggaccgc
    13741 ttcgacggct tcatgtttgc agttccggct gctgtcttct acatcgattt catcaggtag
    13801 caggtggctc atgaacccaa aacgcccaaa aagtgttatt tttgcgggct atagctcatt
    13861 caaagaaatg aaatttacac gttttataca taaagaaggt cgcaagatca tcataggttt
    13921 tttggtgttt ttagtgatcc tcaacttctg cacgcactat tttatgcccg aagccaggct
    13981 gttcaatacc ttagtattga tagcatcgtt gggaggcatc atctttgtga tgtcgttttt
    14041 ccgcaatccg aagcgcagtc tgttcattga agaaggctct atcatagcgc ctgccgatgg
    14101 aaaggttgta gttgttgaag agacctatga gggagaatat tttaaagacc gccgtttgca
    14161 ggtttccatt ttcatgagcg tgaaaaatgt gcatgtgaac agaaatccta tcagtggaca
    14221 ggtaaaatac tttaaatacc atcccggaaa atatttgctg gcttggcatc caaagagttc
    14281 taccgaaaac gagcgcacca cctttgttat tgaggatgag gatgataatg tagaaattct
    14341 gatgcgccag attgcaggta ccgtagcaag aagaatcaaa ttttatgttg atgaaggtga
    14401 tgctgtgaca caaggttccg aattcggctt tattaaattc ggctcacgtg tggatttatt
    14461 cctgccttta gattcaaagg ttgaagtgaa aatcggcgaa aaagtaaaag gcggtcaaac
    14521 agttttagct cgcgtgatgc gtcacaattg atttttttat atctttagta tataatcaca
    14581 acttaatatc atgaaaaaac tgctctcttt cgcattagta acggcctttg gtgttactgt
    14641 tgccatggct caaacagcaa ctaccgctac gactacaaca actgttactg ccacagaaca
    14701 agtgacaact gccgcaccgg taatcaaagc tgttgaagct gagcaaggaa acattaaatg
    14761 caccggaaca ggatcatgct gcagtcatgg tgtaagtcgt tccggagcaa caaccaccgc
    14821 aacttccgaa ggagccggcg tttcaactga agttaccact acaggagaag ctgcagcggt
    14881 tgagacaaca ggagaaataa aagccaccga tgccggatct tgccatcagt tgagcattgg
    14941 ggtgagtgct gcaaaaccgg aaccagctga aaaaactgag gatcctaaga aataacagga
    15001 tacataattt atagaggaga ctgcaatttt cattgcggtc ttttttttgt tcaataatgt
    15061 cagagtaaag cagcaatttc actcaaatgc tgcacttcac gatagccttc tttattttct
    15121 ttctttgcag gattaaacca aataaaagac atcccagcat ctattgaacc tttgccatcg
    15181 acatcggggc tgtcgccaat atataaacat tccgatgctt tagcgggaat aagacttaaa
    15241 gcgtgatcaa atattttctt attcggcttt aagctttgtg cattttcagc agttgtaacc
    15301 gtttgaaagt aatgtctgat tccggtgttt tccagtttaa aaaattgtac ttcatcgaaa
    15361 ccgtttgtaa taatatgcaa gccatattta ccgtgtagat gatcaagcaa ttctcttgta
    15421 tagggcaaaa ggtccgtttg ttttcgggta tgatgaatgt aaaaatcggc agctttattt
    15481 gccagcacat aattatcgag accgaaatcg ttgaaggtga tgtagaatct gtttagtcgc
    15541 acttcatcgc gtgtcacatt tcccagtgcg tattgatgcc acaaatattc atttcgaaaa
    15601 gcatattgct catgaaagcg atggaaatcg ggaatgccat attgatcaag cgaaagatca
    15661 ttatgtattt gtgcgagtat gcgtttagag tttcccgaaa aatcccacaa agtattatcc
    15721 agatcaaata aaatatgatg tatgttttgc atattaaaat tccagaggat gaaatttacc
    15781 gtcagttttt tcagccagaa atgccgctgc ggtatcagca attgtttttt ctaagggtac
    15841 acaggaataa tgaaaagtgt ctttcatttt attgctgtta aagtaagtag ttgcatttgc
    15901 aattcttgcc gtttgctttg taacagcagg ttctttcccg ctgaatagtc cttttaacca
    15961 ttccatacgc catgcaattt cggataatgt ttttccagcg tgcacggaag gctcctttac
    16021 atgcaatgac cttgccatgt gttgtagcac atctttattc gtccaattat cgccattaac
    16081 aataaatcgt tgttcagtga tgtcactgtc catggccact tgtgctatgc gtaccacatc
    16141 acgcacatct acatatccgg tgattccggt ggtataaaat ttaaatccct tataaatagt
    16201 tgagaataat ttactcgatc cattttccca atcgcctgcg ccaaggatta tgcttggatt
    16261 aattataact gcctttagac cttcagccat agcgcgccat acttcgcgtt cggcagtaaa
    16321 tttgctgata gcataatcgg tggtaatata tttcatatcc cacggcgctt cttcagtaat
    16381 tatttcacca tcttttcctg ttcttccgag cgcagcaatt gagctgatat gcacaaattt
    16441 tttgataccc aaatcgagcg caatatttac catattagca gtgccttcgc cattgatgcg
    16501 catcatttgt gcggcactct tttttctgta tgaaatgact gctgcgcaat gatagacctg
    16561 atccactccg gacatcgcat tttcaatggc aaccacatca gtaacatcac caatcaccca
    16621 ttctatttga gaagctatat cttcaaccaa tcgcatattg ctatcggctc tgcgcatgcc
    16681 acgcacttgt ttaccttgct gcagcaacca tctgcagaga taagaaccca caagtccatt
    16741 ggccccggta acaactatca tgatggtaaa ggtgcgatta ttttttcagg atatgcgatt
    16801 acaccgcccg atacggctgt tttagcacct gttactgatt gaagaaaatt tggtttcgat
    16861 tgtatgcgta aagcacccat aagtgccatt gcaagtgctt ctttatactg aataatttta
    16921 tcgcccggca gtgtgatttt tattcctgca ttagccgcaa tgcgttctat cagaaatgta
    16981 ttataagcgc cgcctccggt aaccaataat tgatattgat gcaaaggacg tcgctccggt
    17041 tgcgcaataa taacagcagc aatagcgata gcaatatgtt ctacagcggt agctaattta
    17101 tcatttaaag gaatagtgct tttggcaatg gtatcgataa atgcgcgatg aacaaaatga
    17161 ttatctaaag attttggcca gggcatatca agatatggta cagcatttaa ttgatcgagt
    17221 aaatcctgat caatcattcc tgctgctgcg agctcacctt ctggatcgta atcgagacct
    17281 gccatcgcag cacaataatt taacaactga ttacaggcac agacatcaaa accaatgcga
    17341 ccgctagccg attcaaaaga aatattggca atacctccta aattaagaca agcatcatat
    17401 tgcttaaaga aaataacatc gcacataggt actaatggtg ctccttgtcc gcctgcatca
    17461 atatctgctt gtctgaaatg tgcaataaca ggaattccca aaatatttgc catcaattgc
    17521 ggatcaccga gttgaagact cacaccctgc gcaggaaaat gcaataaggt gtgaccgtgt
    17581 aaggcaataa aatcaatttg gcgatcggga aatagtttca aaaactgcgt aatatgtcgt
    17641 gcaaatattt ctgcaaaaaa agtatgagcc gcatgatgat catcggtaat gacaggaagc
    17701 aatgacttaa tatagctgat ttcctgattg gcgtatttat acgtttcagc cgcaatgatt
    17761 tcaaatgaag attgagcttc cagtttaatt tcacaaagcg caatatctac cccatcgagc
    17821 gaactgcccg acattacacc aattgcagta tatgtttttc tctcggggga cattattttt
    17881 tcagtgtgag tacataatct gtcacttcct gaatttcctg cggagaaagt tgcgctttcc
    17941 aggaaggcat accttttctt ccttcagtta ccacatgtgt aatttcatca tgcgataaca
    18001 aggtgatgct caggttagca gcgccgctga ggccgagatc tcctttagcg ccatgacaaa
    18061 gcttacaatg cttggtatac acgagcatac cgggtgatgc tgtcggctca gcagtagcag
    18121 tatcctgatc ggatgaggag gcagtagtac agctagcaat tgttgccgct accgcaaaaa
    18181 aaataagtag ctttttcatc ggttgtcttc cgattgcaaa gctacttatt cggggtcgtt
    18241 tatgcgacca aattttatct aaccagcgta atatttccct gacgggtgat catatcgccg
    18301 tttgtatctt tagctacgac caaataaacg taagtgccca tttcagcttc cttaccgtct
    18361 atcatgccat cccaagcgtc attaatgata tcagaagtat acaccacttg tccccatcga
    18421 ttaaagatgg taaactgttc aagttcagaa atatcgtgta aaaaggcgct aatgacatca
    18481 tttgttccat caccgttagg tgaaaaggcg tttggcactt ccacaattgg atagtcttca
    18541 acgtagatgg tcacttcatc agtttgcgtg caaccatcgg gactggtgac ggttaacaca
    18601 aacgtagtag tttcagcgag ttctatggta ggattagcta caagaaggct tgatattgat
    18661 gccggtggta ctatcggatt ttgtggatcc caaacgaaat cgccggtacc ggtgccgttt
    18721 aatgtgtagg tttcgagata cataatagta tcatcaacac ctgcataagc atcgggaagc
    18781 ataccaatat agatatcttg ttcgagggta tctgagcatc cgttagcact gtaagctata
    18841 agagtaacgg tatatgttcc gggagcatca tattgatgag aaggctcatc ttcagtgctg
    18901 gttgctccgt cgccaaaatc ccagctatag ctgctgatag atcctgttgt aattgttgtg
    18961 aggttggtga attcggcttc ttcatcctga caaacatcag gagccaggaa gtcgacatca
    19021 ggacccggtt ctacaagaat atcaactgac actgatgact ggcagccgac atcggtagta
    19081 acagttaaga ctacggtata tgttccgcca tcagcatatt cgtgttccgg attagcagta
    19141 gtggaagttg cgccatcgcc aaaattccaa tcccaagtaa tgatatcgcc tgcttctgtt
    19201 gagttagaat tatcttcaaa tgttacaggc gtgcctgagc aacctgcaga ataatcgtaa
    19261 gctgcagtga gtgtattgta taatttaatt atacctatcg cggtatcggc acaaacaaat
    19321 ccggggttaa cgatcagcat aacggtgtaa gtgccggtat ctgcatatac gtgtgttgga
    19381 tcttcaagtg ttgaagtact gccatcgcca aaatcccaga aaaattcagt tccaccgatt
    19441 gaataattat caaatattac agtgaaatca gaacaattta aatattcatc atcaggtgta
    19501 gtgtcgtcaa aagttgcatc tcccacatcc atttgtgcga caacagttgg cgtacatgta
    19561 acaacgttga attggaaatc gcggatatga tctccgagcc aaacgccatc gcgatattct
    19621 ttgcatcgca cacccacaac atatcgacct tctgctgttg cagtgcctgt aaggatacct
    19681 gtaacaggat ctattgcaaa tgcgggtact gcatcaagtg gatataattc atcatatcct
    19741 gtaacccata ccacaccatc atatggaggt ggagcggcaa ttacgggagc cggatcgaaa
    19801 gatgaagcac cttgcagcgg ccaatagaaa tcgtaagcta atgaatcgcc atcgatatca
    19861 gttgcagagt gatcgaaaac aattgggtcg tcgatacaaa ttactgtagg aggaaatgcg
    19921 ttgaacaccg gtgatgtgtt acaagtagca tctgtttgtg gtggaatttc ctgtgtatag
    19981 gttgcaccga tatcatcagg agtttcgatg tttacgatac ctgcatttct gcagcagcgt
    20041 tgaaacacca ataaataacc ctgatcacca tcgggaacgg tgatgatagc agtataaatt
    20101 gcttgctcaa cacatacatc atcaggagca tcaagacaag ggttatcaga ttccgccggt
    20161 attccggtaa taaacggtga atacaatggg aagttattta cataatcgcc atcctgagta
    20221 taaattgtca tgtatgccgg attatcaaat ggcgtgaaac ttgtacaatc gcgatacaaa
    20281 atcaacacaa cctgatattg atttgtaccg atacatgtgt acgatatttc gccaccaatg
    20341 atatgcgatg cgtgtgcctt cagaccaata attgtcatca gggagaggag gagtaacctg
    20401 gttcgcttca tgtgaaaatt ggcttagggt gttaaagata agtaatcgaa cttatctcaa
    20461 caaggtaaca ctaccgctta gacgctgttc cttaccatca catgacgtat aaatgacata
    20521 gatataactg ccaagctctt gcggatgccc atttccgtcg gttccgtccc agcccgcatc
    20581 aatatcgttg gtattaaaga tcatcttacc ccaacggtta tagatttcca tagaaacgag
    20641 cccgtcatca cctgctgtaa tgaatctaaa cctgtcattg accccatcgt catttgggct
    20701 gaaagcgttg acggtagcga agttgaagca gttagggatg atataaacca aggctgtatc
    20761 tgttgaaata cagccgttct cagcggttcc tgtgactaaa tagagggtag taacatccgg
    20821 cagagcgata ggattggggc tgaacgggtc gctgaggcca tcaggaggct cccagttgaa
    20881 agatgaggca ccgttggcat acattgccac ctcctgaccg tggtatacta tcgtatcagg
    20941 accggcaatc acatgtgggg caggcagtat ttcgacctgc atgaaggccg tgtctgaagt
    21001 gcaaccgtcg gtaacgataa cgccataaac cgtgctttca tctggtgaga gccagggttc
    21061 agggctagag gggtcgctaa caccggaagg cggttcccat gcataatatg ttcctcccga
    21121 ggcgaaaacc tgaacactat caccttcgca aatcacggta tcgttcaata atccggccac
    21181 tacctgcggg tttactatga tggtatccag aatgctgtcg acacaacctt tatcagttgt
    21241 tactgtaaga ttgataagat atacacctgc ggtatcaaaa taataatcga aggattctcc
    21301 actaaaatat tgatcaggtt ccatttccca ttgccagtcg acaatatcat ttcctgccag
    21361 cactaccgat gcgtcaaaaa cggtacctga ggtatgcaga catgcgctat cggttgcgac
    21421 atctgcaaag gggaggtgat aaatagtaat ggtgtcataa atttcggcaa tgcagccaac
    21481 actattttcc actgcaaaga tcagcatata atttcccggt tcctcataac tataaaccgg
    21541 attttgttct gagctgcccc atccatcgcc ataattccat tgccatgatg tgatagtccc
    21601 aaaatcagta gtactgatat ccataaaatt taccggtgaa cctgcgcaag tattttcaaa
    21661 actgatgccg ggaattaatt gcggataaat gtgcactgtt gtaaaagtag tatcagaaca
    21721 atccgttccg ggaaatgcga tcaacattac agtgtatgtt cctgtatccg gataagtgta
    21781 aacaggatta tcttcagtgc tattatcatc agttacaccc ggaacaccga agtcccaata
    21841 aaaatcatca gtgccaatac tttcgttatc gaataatacg gtgaagtcgg tacaattatt
    21901 tatttcagga ggtgcagatg ccacaatatc aggtgtacaa tcggtaacat taaattgaaa
    21961 atcgcggtaa tgcgtgccga ttaacacgcc tccacgatat tcttcaactg ctacgccaac
    22021 aacgaattga ccgaggtcgg aaggcgttcc tgcaagccat cctgtagtgc ttcccagcgt
    22081 aaatgcgggt gatgatgcaa tttgataaga ctcattaaat ggcgggacaa aaattacatg
    22141 attaaatggt ggtggagatg caggcgcagg atccggaaga tcataggatg ctccgttgta
    22201 aggtgtaaaa aatttataga ctaaggcatc gccatcagga tctgttgctg cgtggctgaa
    22261 tgcaaacgga tatccggcac ataatgctat tggtggaaga ttattaaact ccggacttga
    22321 attaattaat gttcccggat cgggaatatg ttcggtatag gtagtacctg tttgaatagg
    22381 atcataaata ttgatgatag tactattcct gcaacagcgt tgatacacca gatcataacc
    22441 accaatactt ggaggcagat taatattccc gacataaatt gcttcctgca cacagagatc
    22501 cggagcaacg gtcatgcaag gatttacaat ttcgggatca atattatcgg aaccgggaaa
    22561 aaataatacc agactatcaa caagaatgcc tgatgcatta tatacataaa tttgcagtgg
    22621 atcgtcgaaa ggcgctgctt cgggatttga gcaatcgcgg tagattttca gtgttatctc
    22681 aaaatcattt cccgtgagtc gcttataaat catttcacca ccaatgatat gaaaggcaaa
    22741 caccttgttg cacaaggcga gcgctaggat caatggcagg tatttacgaa aaataaaaag
    22801 ggtaataaag atacgctgaa aatactgcga atgttcacgc cggtgtcatt ctttgaggtt
    22861 tagacaaccc aaatcaacca gggtttatta aaaaagcaaa acaggccctt tactgttaaa
    22921 gttacttaac ggcactagta gggatttgat acacctgatt atcttagcgg aacgggcatt
    22981 acccaattaa ccgaatctac ctatgcagaa aatcttactt accgcactaa gtatacttgg
    23041 tgcaagcatc atttacgggc agaccacttt tcctgttaat ggtgtttatg atgagcgatc
    23101 acgaacctat gcttttacgc atgccactat tgttactgat ccgggcaata ccttacagga
    23161 tgcgaccttg ctgatcagcg acggtcgtat tattgatgtt ggcacagcag tagttattcc
    23221 caaaaatgcc gttgttgaag acctgcatgg caagtacatt tatccggcct ttatagatct
    23281 ggatgcaggg tacggaatgc ctgcaaagga aaatccacag gtaggcagag gtccgcaaat
    23341 gttaaacagc aatcagcagg cagtaagttg gaatcaggca ataactcctg aatatcaggc
    23401 cgtttccgga ttcaaacctc agggtgatgt tgctaaatta tggcgaagca gtggttttgg
    23461 actggtgtta acacatcgac atgatggtat catgcgcgga accggtgcac ttgtatcgtt
    23521 gggcgatgag cgtgcaggtg agatggtagt taaaccaaat atcagcgatc attattcgtt
    23581 tcgcaaaggc acatcgacac aagaatatcc gcagtctgag atgggaagta ttgcattgat
    23641 aagacagaca ttttacgatg cggactggta tggcagggtt ggaaaaaatt cagaacagaa
    23701 tatatcattg caagcagtgt tggatcaaaa aaatctgatt cacttttttg ataccgatga
    23761 gaaacttgaa attttgcgtg tagctaaaat cgggaaagaa ttcgggtata atttcattat
    23821 gatgggcagc ggcaatgaat atcaacgcat caatgaaatc aagcaaacag gtgctgtttt
    23881 ggtattgccg ctcaattttc ccaaaacacc tgatgtgagc gacccctggg atgcagaatt
    23941 attaagtatc agtgatttaa aacattggga aatggccccg gcaaatccgg caattaccga
    24001 gcaagcagga attacatttg cacttaccag ttcaggtctc gatgataaat cacaatttct
    24061 gccaaatgtg cgcaaagcat tacagtatgg tttgtcagaa aaaacggcat tgtcggcatt
    24121 gacatctgtt cctgcacaaa ttttaaagtt agaaagcgaa accggatcac ttgcaaaagg
    24181 caaacgcgct gattttttta tcagctcaga taatattttc cgcgaaggcg ctatcattta
    24241 cacaaattgg gtaaacggtt tgccttatgt cgtaaattca gaaaatagtg atttgcgagg
    24301 tgcctatgct ttgcatattg ataatatggt ttacggacta ttaattcaag gtaaaataaa
    24361 tgctcccgaa ttttcgataa taaaaaatac agacaccctc aaagcgcaag gagccatttc
    24421 aactgaaaac atacagatac aatttgcaga taacggcaca ttatatcgac taaccggata
    24481 ccacgatggg aagaatatag agggacaagg caatgcgaat gataaatgga tcaattggaa
    24541 agccgaattt gcaagcgtgt atgctgaaaa gccgacacct cagaaaaaag atactgtaca
    24601 aattggaact gttatgtatc cgtttactgc ttatgggtgg gaagcagcac ctacacagga
    24661 aaatattttg ataaccaatg caacagtctg gactagtgag aaagagggcg tgttaaaaaa
    24721 tacagatgta ttaattggca atggaaaaat tttgcaggta ggaaaaaaca tcagtgcaaa
    24781 aaatgcgcgt attattgatg gtacgggtaa acatgttacg cctggaatta ttgatgagca
    24841 ctcacatatt gctgttactg atggcgtgaa tgaagggaca caaaacagca gtgctgaagt
    24901 gcgtattgct gatgtaatta attgtgatga tatcaatata tatcgacaac tgagtggagg
    24961 tgtaacaagt gcacatatat tgcatggctc agcaaatccg gtaggtgggc aaacacaact
    25021 tatcaaattg cggtggggtg ctgcacctga ggacatgaaa ttcgcgcaat ggccgggttt
    25081 tataaaattc gctcttggtg aaaatgttaa acaaagcaat tggggtgatg attattcagt
    25141 gcgcttcccg caaacaagga tgggtgttga acaagtgtat gtcgatatat ttaccagagc
    25201 ttcagaatat gccaaggcga aaaaagaaaa cccacaatcg gtgcgcacag atctggagtt
    25261 ggaagcaatt gcagaaatac tggaaggaaa gcgatttatt acttgtcaca gttatgtgca
    25321 aagcgagata aatatgttga tgcatgttgc agattctttt ggctttacga taaatacctt
    25381 cactcatatt cttgaaggtt acaaggttgc cgacaagatg aaaattcacg gtgcaggagc
    25441 ctctacattt agcgactggt gggcatacaa atttgaagtg tatgaggcaa tcccacaaaa
    25501 tcccggaatc atgcatgatg caggattagt taccgctata aattctgatg acgcagagat
    25561 gggccgcagg ttaaatcagg aggcagcgaa atcagtgaaa tacttcaaca tgtcggaacc
    25621 ggaggccttg aaaatggtaa ctattaatcc tgcgaagttg ctacatgtag atgacaaggt
    25681 aggcagcatt aaagtaggca aagatgccga cattgtgatt tggagtgaag atccattgag
    25741 tgtttatgcc atggcagaaa aaacaatagt tgacggtaaa attttatatg acagaagtat
    25801 acaagatcaa ttaatggctg cagaagatgc agagcgtcat cgcatcatca ataaaatgat
    25861 ggcagaaaaa acaaatggcg cgatcactgt tccgatagtt gtaacaccac atcgcgaata
    25921 tgactgcgaa gatgtaattg acttcatgaa aggcgaataa caagtaaaaa aagaaattat
    25981 tatgactcga tatattaaac aattattgac catagcattt ttgctgcata tagttgcaac
    26041 tcatgcacaa caggttccag tagctatttc cggaggacgc atacatgttg gtgatggcac
    26101 agttattgaa aatgggctag ttatcattca aaacggcagt attcagtatg ctggtccgat
    26161 gaaggaaagc ggctatgacg caaatacggc atcattgatc gacgcaacgg gcaaggatat
    26221 ttatccggga tttataggca tgggctcaac aataggcctc aatgaaatta ctgctgtcag
    26281 gtcgacacga gacgcttatg aagttggcgt gtacaaccca aatatcagat cgttaatagc
    26341 ttacaataca gattcaaagg ttattccgac tgtgcgcacg aatggtgttt tgcttgcaca
    26401 agttgttcct tacggtggtg taataggcgg acaaagcagt gtatttaaaa tgagcggttg
    26461 gaattgggaa gacgctgcca ttgcaaccga cgacaacatg catatgtcat ggccatcggt
    26521 gtataattac gactatgaaa aaaacaccta tggccctaat gcgcaatatc gaagcgaggt
    26581 agaggacata tatacatttt ttgctgaggc aagatcatat tgcaatggca atgcacctcg
    26641 cgagcgcaat ttgcggtttg aagcaatgcg aaaagtgttg aacggaaaac aaaaattatt
    26701 tatatcagca aacagtgcaa aacagattat tggtgctgtt gcgttgagtg attcttttca
    26761 tattaaaatt gtaatcaggg gtggaaggca gagttatcgt gttactgaat tattgaaaga
    26821 aagaaatatt cctgttttat tggaggcaac acatgaatta ccttcctatg atggcgatcc
    26881 ggttgatttg ccatataagt tgcctgcaat gttgcatgat gccggcatta tgtgcggact
    26941 ttcggtatgc aatgaaggtg attcttactg gaatttgcgc aatcttcctt ttcaggccgg
    27001 cactgctgcg gcatatggtt taaatagaga agaagcccta aaaatgatta cgctgaacaa
    27061 tgctatgcta gccggtattg acgaaaaata tggcaccata aaaacaggaa aaagtgccac
    27121 attgtttatc agcgacggcg atgcattgga tatgggaaca aacagactta gcatcatgtt
    27181 tattgccggc gagcgcttaa aacccgacaa ctggcaatct gaattgagta acaaatacga
    27241 gctcaaatac aatatccaga tccatcgttg attcgattaa tctttagaat aagccccgga
    27301 agtcgtacct ttgccatcct ttcagccgga gagatgtccg agtggttgaa ggagcacgcc
    27361 tggaaagtgt gtatacccct aaagggtatc gagggttcga atccctctct ctccgccaaa
    27421 aaaatccaat gcagcctacc gcaggctgca ttttttattt caaaaggatg tgacaagttt
    27481 acttggcgca gcattttgaa ataaaaaatg cagatgagcg cagcgaatgg ctgcatggta
    27541 tttatttgaa gccccccatc tcaagggatc atgataataa tccctcgtcc ctcctttcaa
    27601 taaaaatatc aattgagcgc agcgaatggc tgcataccat ttgtttgaag cccccatcta
    27661 agggatcatg taaataatcc ctctatctca ttcaaaaaaa atgcagatga gcgcagcgaa
    27721 tggctgcatg gtatttattt gaagccccca tctcaaggga tcatgtaaat aatccctcta
    27781 tctcattcaa aaaaaatgca gatgagcgca gcgaatggct gcatgnnnnn nnnnnnnnnn
    27841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27901 nnnnnnnnnn nnnnnnnnnn nnnnnaatcc ctctatctca ttcaaaaaaa atgcagatga
    27961 gcgcagcgaa tggctgcatg caatttgttt gaagccccca tctaagggat catgtaaata
    28021 atcccactac tcaacaactt taacatccaa ccctcaaccc tcaaccccaa acaacctcct
    28081 tgcattctcc gaagtaacct ccgcaacatg ctcgacaggc aggccaagaa tttcagcgag
    28141 tttattcaca atttgcacta aataagacgg ttcattccgc tttcctctaa atggaacagg
    28201 agataaatat ggagcatcgg tttccaatac aaggtgagga agatgttccg gttttagcac
    28261 tttatcgagc cctccgtttt tataggttac cacaccacct attccaatat aaaatccaag
    28321 atcgatagat tgttgcagtt gcaattctgt tcctgagaag caatgaaaaa tgccgcgaag
    28381 gtttgcgtct ttatgtttgc ttacaagatc gatgcaatca tcagtggcat ttctggaatg
    28441 gatgatgaga ggaagctgca tttccttcgc ccatcttatt tgagtaacga aagcagcctt
    28501 ttgttgatcc acaaaagtga gatcccaata ataatcaaga ccacattcgc cgattccata
    28561 ccattgatat tgtttgtcaa ggagcaattg atgtatctta agcataacat catctgcatc
    28621 gttctttacc gaacatggat gaagtcccat cattggcttg catgcgcgat ttttcttcgc
    28681 aagggccatc atggcatcta cagattctaa atcgatattg ggcatcatca tgtgcgaaac
    28741 acctgattga agtgcacgtt ggatcatgtc gtctaaatcc gaatcaaatt caggtaaata
    28801 aatgtgggta tgcgtatcta ttaattcggg cacaacgcta aggtattcaa atcatgacaa
    28861 atggaggccg cgaggtaacg atgcagtgtt tgcaggcagg gattcaaagc gatatccttt
    28921 ttcgagaaaa tgttctatca ccacaggtaa tgccggcaac accctatcgg cagctttttc
    28981 actgtcgtga aatacaatga tagatccatc ggcagcgtgt tgaattacat ggtctatgca
    29041 atcttgcggt ttcagttgtg tgtcaaaatc accactcaat acatcccaca taataatggc
    29101 atattgccgt ttcaattcct gaatttgctg gtaaccgatt ctaccatatg gtggccggaa
    29161 caattgcgaa ttaactaagt tactgcaggc tgatacctct tcgatataag catcggtatc
    29221 tgttttccag ccattgacat gatgaaaggt atgatttcct gtcatgtgac cttcctgcaa
    29281 aatttgttga tagagttccg gatgttttct gacattatcg ccgatgcaaa aaaaagtcgc
    29341 tttcacatca tatttcgaaa gtatgcccaa caccttatcg gtaacaccgg gaacagggcc
    29401 atcatcgaag gtgaggaaca gcctgttatc tttgggcata tcccatacca agctggggta
    29461 tacacttttc aggtaccagg gtgtcttcac aaaatacatt gtacaaaggt agtttgattt
    29521 gacagtattc actatacatg gtttaagacg gaaacacaca ttagtgtaat ttcaccaagc
    29581 cgattaactt tgcatcacat tacaacagta tggatagacc aataagattg cgctatgcac
    29641 caagtccgac aggagcacaa cacctgggag gaattcgcac ggcgttgtat aattatttgt
    29701 ttgtgaagaa gctcggtggc aagttaatct tacgtatcga agacacagac cagacacgtt
    29761 atgtgccggg agctgaagaa tatgtgatca acagttgcgc atgggcggga ataacatttg
    29821 atgaaggtgt acatatcgga ggaccatttg caccttatcg tcagagcgag cgaaaagaca
    29881 tgtatcgaaa atatgccatg gagctgattg aaaaaggtca tgcctactat gcattcgata
    29941 cacctgaaga acttgaggca atgcgcaacc gcatgatgga gcagggaaat ccttctccgg
    30001 catatgatca catcacccgt cagtacatga aaaattctgt tgctttgtcg aaggatgaaa
    30061 ctgagcgaag aattcagaat ggagatcctt atgtgatacg tataaaaatg ccaagaaatg
    30121 aggaagtaaa attccacgac cttattcgcg gttgggtaag ttttaattcg tcgcaattgg
    30181 atgacaaggt attattcaag agcgatggta tgcctacgta tcatttagcc aatgttgttg
    30241 atgattatct tatgcaggtt acacatgttg tgcgtggtga ggagtggtta ccaagtgccc
    30301 ctacgcatgt aatgacatac cgcatgtttg gttgggaaga ggtgatgcca tcctttgcgc
    30361 atttacccct aattctaaaa ccggaaggca atggcaaatt aagtaaacgc gatggcgaca
    30421 aatatggctt cccactttat tctctagact ggaaggaaca aaatatttca ggcttgaagg
    30481 aaaagggatt cttacctgaa gcgtatatta actttcttgc atttttagga tggaaccccg
    30541 gaacagatca ggaggtattt accgaagagg aactgatagc cgcatttagt tttgagcgca
    30601 taggaaagtc cggtgccaga ttcgacccgg aaaaagctaa gtggttcaac cagcagcaca
    30661 tgcgcaggaa aaccggcagt gaactggcag acctgttaca accatatctt gataaaaagg
    30721 gtattcaagc tgaccgaaac tacctggaaa gggtgtgtga attgatgaaa gagcgcgcgg
    30781 ttttcatccc tgacctatat gaggcgggta catttttctt tggtgagata agcgagtatg
    30841 atcaggatac catcagaaaa aaatggaaac ccgaaaagca ggaaattttt ctaaatttga
    30901 tcgcccatat tgaatctatc agtaatttta ctgagccatc gatagagtcg ggggtaaaag
    30961 actatatgaa agaaaacaac cttggtatgg gtgaagtgat gccgctttta cgtgtggcgc
    31021 tggccggagt acttcaaggc ccccctgttt tcgcgatgat ggatgtatta ggaaaaaata
    31081 tttctgttca acgtttgcat actgcaatta aaatatttga taactttcgc tgaaaatcag
    31141 agtaccctat gcccaagaaa aagccattgg aaggcaaacc tgaagtgcat aaagatttag
    31201 atggatttga gatgaacatc aatgaattcg gtgagataac cagcaacacc gacattgata
    31261 aactcaatca gtttctaaat aaaaatgtag acgacaaaaa atttcgcggt ctcgataacc
    31321 tgccttatgc caagggaggt gatgatgatg aggaatctac cgaggaaaag gatgatttaa
    31381 atgaaatctc cgatgaattt gaggagttcc gtcaatttaa tgacaaggac ttcaaagatg
    31441 aattcgactt cgatgaaaag gatgaggctg atggctattt caacgaaggc aagcacgaat
    31501 acgaacagtt tgaggatgag gaagatgacg ccaaagctga tgatgaagaa gatgattttg
    31561 atgatgaaga attccttgac gattacgctg atgactatgg tgaggaagat aattacaatc
    31621 tcgacgaagg agatttcgac gataaatact aagcttagac caaaacagtg tttctaaata
    31681 tcatgcaccg gcggcaaacc ggtgcttttt tattttatta aaatgccaaa tagaaatcac
    31741 ctgtcaactt tttgtaagct tctgaatagt taccattagc atcatatatt gtcaacacat
    31801 cagttgaagt gcctgattca cttctcccaa aagcaattaa aacgcgcttt gccggcttct
    31861 cgggttttgt aagcactgtt gtaatgcgat ttgaaaacca accattaatt gcagcagttt
    31921 gcaggcaagt atccaatgcc agggccggga gaatcagaca aaaaaccgta tgctgcccgg
    31981 aaatttttgc agcacgttga aataaatcca caagcggtaa tgaagcagca tcccttgcct
    32041 tacggcgccc ttcatcttca accggccatc ccttttcata ataaggcgga ttggatatta
    32101 tcaggtcata agcagatgac ttttcatcca attgcaaaaa atcactattt ataattttaa
    32161 tgcgattact ccagggagag cgttttgcat tttcaattgc ctcgaaacag gccgcttcat
    32221 caatttcaac cccggtgatc agggcttcag gaaatcgctg agccatcatt atggaaatga
    32281 gtccggttcc gaccccgata tcaagaatat gttcaacctt tcctttactg aacgcccagg
    32341 cacctaacag cacgccatca accccaactt tcatggctga atcttgttgc agaatggtaa
    32401 actgcttgaa gttaaacgat gacattgcgt taaaaatgag aaaaaaagct gatttctgag
    32461 gatgtagtgt taactttatt agctaaacat tgatgatgaa aaacaaatta ctcctcctta
    32521 cgtcaggtat tttgatgttt tcaggtgcct ttgcacagtt aacgttaaca cctgagccaa
    32581 atgatactac ctttatttat tctctggcag gaccgggaat agtggtatct aatattgaga
    32641 gaaattgcca tacagatgca tctgcatttt acaatagcac ggatgcgaat gtgggtatag
    32701 aaaatggcat tctcctttcc tcgggaacag cggcctcatc ggcaggacca aatgatgttg
    32761 gcgcagcctc ttcctattta gattatcccg gagatactga tcttgaaggc cttaccatgt
    32821 ggctcacgta cgatgcctgt gcaatatcct ttgatatgac cgtgatggca gataccgtta
    32881 aattgaaata tgtgttcgca tcagaagaat atcctgagtt cacattttca gggttcaacg
    32941 acatatttgc attttgggtg agcggtcccg gtatcccaac gccggtaaac attgctactg
    33001 ttccgggcac agacttaccg gtatcaatta acaacatcaa cgctacaact tattcagatt
    33061 attatatcga aaatggcgat ggctatatga cgccatatag taccgatgat tactatattc
    33121 aatttgatgg cttaacaaca gttcttgaag ctaaactagc cgtaaccgca ggtgaaacat
    33181 accatatgaa aatggccata gcagatgctt ctgacggtat ttatgattcc ggcgttttcc
    33241 ttgaaacagg aagccttgga agtcttcgca tgcataccga tgtgtttgca gataatgatt
    33301 taacatttgc tgtagaaaaa tgtgcatcag gacatttcac tttcacaaat gagatcccat
    33361 cagcagaacc attggtaata gattatatga tcggaggatc agcaattaat ggtgttgatt
    33421 atgaaacaat tcccgaacag ctcaccatac ctgcgtggac atcagaaggc actattgaca
    33481 ttgttcctat tcacgatgta attgatgaga gctttgaaag tgtaattctt tatctataca
    33541 atcctcagtc aggcgttatc tacgatactg ttaccatgct catagacgat gaaccggcag
    33601 cagcctcttt tgcatctact atttcagatt tgagtgttgc attcaccgaa acaagtggtg
    33661 ctgctgtatc gcaatactgg gagtttggtg atggcaccat atcgacagaa tcaaacccga
    33721 cacatattta tgcaacagcc ggtgtttaca atgtttgtgt aacaggttat aatgcttatg
    33781 gatgttctga tacctattgc gatatggtaa ctgttggtaa tgttgacata aatgacttac
    33841 aaaacacctt aagcatttca ccaaaccctg ttgcaaatgt gctgcaatta gaaggaacag
    33901 aaaataatgc ggtaatcaat atattgaatc tgcaaggtga agtagtgctt acatcaactg
    33961 cttacggaaa ttctgtatcc attgatgttt ctgcactgcc tgcaggaatc tatacgctta
    34021 gggcaacaca aggtagtcaa atggcaataa gtaattttga aaaattataa actataacac
    34081 atatttaata aaaaaaccgc atctaatcgg atgcggtttt tttattgcca tttatattct
    34141 aacaaacttc gatgtataca tcttttcacc ttgcttcaca gcaatcacat aagaaccggc
    34201 tgcgaggcca gaaaccggaa tgttatatgg catgtcttta tttatggttg cctttgtttt
    34261 atatacaata tcgccttgca tattcacaac agtaacctca gcattagaga aataagcagg
    34321 aatggtgatt tgaatttttt cggaggccgg atttggatac acgctcatta atgcttcctg
    34381 attggcatca tgaatattta tggtaggtaa tttttctatg gcaaattccc aggcacctct
    34441 gccataagtt ccaaagcgaa cggtattaat ttcatcaaca taatcgacgc tccaataggt
    34501 ttgcagtgga gcatcattag ctgctatatt ataccattta ccttctgcaa gtacacaaac
    34561 gtaaggtgca acttccgttg ccgcaaacaa caggctgtcg gtttgatcaa cagccagatc
    34621 atatactagt gttggaggga ggccatcttg taattccaca aaattgtcgc catgatcgtc
    34681 agacatataa actgccggat tatcataacc acttccaccg atatacacta ctccaagttg
    34741 cgatttagat ggttcgatag ttgcaccata aaaatattgc ggtccgggac ctgaaaatcc
    34801 actacttaaa taccaggttt caccggcatc ttccgaatga tagaaatttc ctccactggt
    34861 aagtaaatac caataattgg ggtcaatagg agaatagctg attgctgaaa tagaaccacc
    34921 gccggcaact gaaaagtttt ttgcctgttt cacacctgaa atgaatgcac cacttttttg
    34981 caaacgccac aagtaggcac cgccggcatc ggagccccca ccccaccatg ccacctgatc
    35041 gttccacgga tcttgcatga taggcgccat ccaaaggtga ccataaccct gaaaatccca
    35101 tgtagtaaga ttggatgccg aggctgcatc attcacatac atggcaaatc ccggataaac
    35161 gcaccataaa ttatcaccac cgcttcttga aacaatatga ccataatcgc cactaatcaa
    35221 ctgatcaaaa taatagtcat caccaatagt ataactactg cgttgatatc cctgatcctg
    35281 cgcccctgca aacaaaatat cggttaccca tcgataggta tacacatcat aatattgagc
    35341 attgcgcatg ccttcgttag tgatattatt ccagataagt ccattatctg ttgatttata
    35401 taagccgcca tcagtactga tcaaaacgat ttcatctccc ccaccatcaa taaaagttct
    35461 gataaatggt atatcagcgt gcagattata ttctatatcg ccataatatt cgtaccaata
    35521 attattccgc acccatgaat taccggcagt aaaactccta tagctatcaa caccaccata
    35581 aaataaatat ccctcctgtt tctgagagca attaaaacta ttcttactga aattcccttc
    35641 ctcaataata ccctctgtgt tccaatttac accgccatcg aaagaagtat aaaaatgtgt
    35701 gaaattatcg acatagtttc tgtgagccac atacaggctt gtagaggaag gtccctcaaa
    35761 accacacaac atataatcgc catcatctgt tataggcagt gttccttgca atacaggtgt
    35821 tccggtggca tcgagtaaat taacgtgcgt attttctatc atatagcaat caggagagcc
    35881 gtatggcggt gaccacaaaa cgaagtgatt ggccccacca taaacaggaa catcatatga
    35941 gctgagctta gtgaatgaaa ctcctaaatc ttctgagcga tatagcgaaa cgatatccca
    36001 ccaatttgta taatcccatt cataagccaa taaataaatg gtgtgattgg catcatttaa
    36061 tatttccata tcagagatat agccccatgc tgcaacattt tcaagaccgg ttgttgcatt
    36121 ccaggtaagc ccttcgtctt cggtataaaa aacgccttga acaccccatt ggtcgcaacc
    36181 tacaattaat ctatcaccaa tttttcgaag cgtatgtgct ccggttatct tcatattatc
    36241 attgataacc gcccagtttt cgccatccag atcgcctttc caaagattac cgccgtcgga
    36301 aaatgcatat atagtttcat tttcaaaatc tacttctgtt gctcttattc ttccggaggt
    36361 attataagaa cccatctcat tccattcgcc aattaatgct cctgatgcca gggtatccat
    36421 agcgatatca tcggtgcgaa gattattact gcgctgtttc attaactgaa atcgcaaatc
    36481 ggcatccatt tttctccaat ttgttccggg tgcagattta tgcatttggt cgaagaaatt
    36541 ttgtctgtcg cgattatagg attcatcttc cttctcatga ttaccatcca ccctgacagg
    36601 tgcgggattc gtctctgtgt gatggaaatt attatcatct ttttgcaaaa tgaaaacaat
    36661 agccatgcta acgaatgtta acaatatcga cccgataata atgatgattg caattttttt
    36721 attcatcgta gtatttcttc aataaaattt atttaccatt tgccacttca ggtataacat
    36781 gacctataca tgcgtttggc tcactgatgt ttaacagatc ggcaagtgtt gcagcaatat
    36841 cagtcacata tacattccga tgcagtgcac cggattttat cttccatcca taaaaaatca
    36901 gcggcacatg tgtgtcgtat gaaaaatgtg atccatgtgt tgtacctgtt ggcctgtagg
    36961 cttcaaacca ataaggttca tacagaattt gaatatcgcc acatcgtttg ggatagatgc
    37021 cattgatgac aagctcttta acatcattgt tcatggtggc gtcattcaat ttattaattt
    37081 gtaagacata agaaacgccg tccattttca taagttgggg gtagagaaca ttatatgcat
    37141 cttcaatagt tttacccgca gcttgcaata atgcatcatt taaataaatc tgctggttgg
    37201 tgtatgcact tatccagtct ttttgacccc atgcggcatt caacatcgct tgcatacttt
    37261 gcaacatcga cacttctgta gttgttccag ccggaatgcg caatgattcc atgtattgtg
    37321 gtgttggtgt aacaccatga tctgcagtca gaaataataa ataatttcct ttgccaactt
    37381 gtgtatctag ataagtaagt aattcaccta agtctttatc caaccgcaag taggtgtctt
    37441 gaatctccat agaatgcggg ccgtatgcat gtcctacata atcagtagat gaaaaactta
    37501 cacataaaaa atccgtttca ggacccgtcc cgagttcttc attcttaata gcagccatag
    37561 caaattgcgc agtcaatgta tttccgaagg gtgtaccttt tatactttcg ctattaccgg
    37621 taaatggcaa ataatgcggg aatttcggcg atgtttctcc ggtgtacaat gtctcccacg
    37681 aaacagaatc ggctgtactt tgattatatt gagatgcagg caataagaga tcccaatttt
    37741 tattcatcat tttttgcgcc ggttgctgca tattaaatgc actcacccag gtaggcaatt
    37801 gttgcatata ataggtactt gtcacccacg cattactggc gccatcatac caataagcgg
    37861 catcagctgc atgccctgcc ggcaatatcg caccgcgatc ttttaatgca attcctatca
    37921 ctttcgatcg ttgctgattg ctcaactgca acgcatcagt aatggtagtg gccaccatat
    37981 ttttcggtga cattttactt cccagtccat tgccaccaac cattgatact gtgctgtcgt
    38041 cggtaacgta aacatatgcg ccgctctgtt cattgaacca atcgttgctc actacgccat
    38101 tgagtgcagg aaccgatccg gtataaatgc tggcatgacc gggaccggta tacgtgggaa
    38161 tgtagttata atgggtgttt tcgcagctat agccatctcg caaaagtcgc ttgaaaccag
    38221 aatctgagta tgtatcgcta tagcgatata aaaactcgta ggccatctga tcaactacga
    38281 ttccaaccac cagtttaggt ttcgaaggaa tagtttgtcc gttcattgtc aaaacagacc
    38341 caagtatcaa taatgaaaac aatcgcttca tgcacaaatt taattagtaa ttctcaaccc
    38401 aagagttggc cgtccgtcgt ctcccaagat tgccgacaag ccttcacttg agcgctctta
    38461 atatttcgtt tacttttctt ttacaaagta tcaaaggtta gccctgctca tatacgtagt
    38521 tttggccatt caaaaattca tccaatggcc agatatttcc tattccttac tattgtatgc
    38581 atttctgcag ccctttcagc ccaaacggct gaaactgaca ccagcaaact gaagttgttg
    38641 cctacggtaa atgtacaagc tgaacgcacc attccggata ttcagcgtat acaacctgtt
    38701 aaagggacat ttatttattc aggcaaaaag acggaagtta tcgatctaac acaaaggact
    38761 gtgtcgctga gcgagaagta tggtcgtcaa atattttcaa aaatacctgg tgtatttgtt
    38821 tatgacatgg acggcaccgg caatcaaata aacatttcaa ccaggggctt agatccgcat
    38881 cgcggttggg agttcaatat cagaaaggat ggtgttctca caaactccga tatgtatggt
    38941 tatcctgcaa gtcactataa tatcccgatg gaagcagttg agaaaattga attagtgcgt
    39001 ggaacgggat cattgcaata tggtgcacaa tttggtggta tgttaaacta catcagcaaa
    39061 caacctgatt ctacacgtgc attggctttt gaaacaagca ataccgtagg ctcctacggt
    39121 ttagtaagca cctataatgg cgcatcgggt aaaattggaa aattcagata ttctacctgg
    39181 tataataaaa aatggattac aggatatcga gataacagcg acgcacaata tgatgctgaa
    39241 gctgtttcat tattttacga ctattcaaaa gatctgacct tcaaatttga gtggacgcac
    39301 tccaattata ttacacatct tccgggtcca ttaaccgaca gcatgtttta tgccgacccg
    39361 caaatggcta cacgttcaag aaattattat aaccctaata ttcatgttcc ttcaattaac
    39421 atgaattgga atataacatc atctactaaa ttacagctaa catcatcagc ggtaattggc
    39481 gccagaaaca gcgtgatgtt tgacagacct gccaccacag aagataccat tgttggagca
    39541 acactcgatt acaataatcg tcaggttgat attgatcatt tcaacagcta cacaaatgaa
    39601 ttgcgtttgt tacaatccta taaattatta ggtcatacca gcaatctcgc agcaggtgtg
    39661 caatacatgt ataatgattt acatcgtcag caaattggca aaggcactac cggtagcgac
    39721 ttcgatctta cattggctat accgggctgg ggacgagatt tacacttcat aacaagcaac
    39781 attgctgtat ttgcagaaaa caggtgggtg ctctctgata ggttttcggt taacaccggc
    39841 gcgcgaatag aaatgggtga gagcaatatg agtggtgtaa tcacatatta tcccgattca
    39901 actttgccaa acaccattaa acacagcttc ccattgtttg gtgtaagtgc acaatacaat
    39961 gtatctaaat tcaccaatat ttatggagga tggtcacagg catatagacc ggtgattttt
    40021 aaagacatcg tacctgcttc tgtatatgaa tatgcagata aagatttaaa agatgcatac
    40081 ggttacaacg ctgaggttgg atatagagga aactggaaat ttttaaaatg ggatgtaacc
    40141 gctttctact tagcatataa caatcgcttg ggaacattgg cagaaacaga tacagccggg
    40201 aatctgatta tccgtaggac aaatattggc gactcccgaa caaccggtct tgaattcttt
    40261 atgcagggcg attttgatct cacaagaaaa ttgtctttat caatatttac atcaacttca
    40321 tatatggatg ctcgctacca tgatgcaacc attcgttcag gcagtgaaaa tatcgacatt
    40381 gatggcaata aagtagagag tgttcctgat attatttccc gcaacggact gacattgaaa
    40441 tatactcgat acagcctttc agctttatat agttatgttg gcgaaagtta tgccgacgca
    40501 ttaaatacgg tagaaccaaa tgccagtggc tctgttggcc tggttccttc gtatcaactc
    40561 atcgacataa atattggcgt taaactgact aatcaggtaa aagtgcaatt caatgcaaac
    40621 aatattcttg atgaacagta ttttactaaa cgtccgcaat tctatcccgg tcccggtata
    40681 tggccttcag atggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatatca
    40801 ccttctgctt caattttctc aaaaaaacct gctgcaccgg aggcctcata ttcggtggga
    40861 cataaataga tgacagaata ttcatcggct tgcgattcat cgcctctgct gaatgcaagt
    40921 tgagtcaggc ttgcagcaat atcagtaatt gcgttttgat catcacccag cacaatatcc
    40981 aacagtattg atataccata agcctgagta agctggtcgg tagaatgcct caggtcggca
    41041 tgtgccattt cgtgaccaag cactcctgcc agttgagctt cattctcaag gtatctgatg
    41101 agccccgtat acacatagat gtaacctccc ggggtacaaa atgcattgat cacggaatcc
    41161 tgatctataa tacgcacctg ccacttaaaa tcatccttgt gaaacaccat accactctca
    41221 aggattctat cacggatatc ataaacgaag gcataagcat ctgcatagtc gacgctatca
    41281 aggataggat aatttacagg atcgcctgct atttctgccg ctatctggtc tcccaaggct
    41341 ttgtcgtctt caatagaaaa aatattgatc ccaccccctt cagaacagga ttgcatggca
    41401 attatgccgc tcaggaacat accgaagcag cttacaataa caacagttct attcacttta
    41461 aacataaaaa acgagaggct ctcaacatga tgcaagcgcc cctttcacat gttaaacgga
    41521 aacagaggta ttttgttaca tctaaaaaat gtgccttaac ttgcagcccc atggccgata
    41581 tcgcctccat tcagcagcca attcacgagg agttagccct ttttgaagag aaattcaggg
    41641 cttctatgcg cagcaatgtg gccttgctcg atagggtgat gtattacatc gtaaagcgga
    41701 agggcaagca aatgcgcccc atgtttgtat tgctgagtgc gaaaatgatc ggcaatattt
    41761 ctgaatcttc ctatacagcc gccacattaa ttgagctcct tcatacagca acactggtac
    41821 acgatgacgt ggtagatgat gcctatgagc gaagaggatt tttcagcatc aatgcgcttt
    41881 ggaaaaacaa aattgccgtg ttgatcggag attatttatt ggcaaaagga ttattagtgt
    41941 cgctcgacaa taatgaattc cgaatgctaa agattacttc cgatgccgta aaagaaatga
    42001 gtgaaggaga attactgcaa attgaaaaag cacgtcgcct cgatatcaaa gaagatgtct
    42061 attttgagat tatcagaaag aaaaccgcta cactgattgc cagtgcatgc agtgcaggtg
    42121 ccgccagtgt aaccactgat gaagatacta tttcgcttat gcgtcttttc ggagaaaaaa
    42181 ttggcattgc atttcaaatt aaagacgatt tacttgatta cggcaccgat gatatcggca
    42241 aacctcttgg tatcgatata aaagaaagaa aattaacact tcctattatc tacacacttt
    42301 tacatgctcc aaaaaatgac aaaagtgaga ttatgcgcat catcaaacac tacaataccg
    42361 acaaggaaaa agtgaattgg gtgattgatt atgtaaagaa gggcggaggc attgcttatg
    42421 caacagaaaa aatgaaacaa taccaacaag aagccttcga actacttgaa aaatttcctg
    42481 aaagcgagag caaacgcgct ttaaaggatc tcgtaatatt tactactgag cgaaaaaaat
    42541 aaattgttgg tttactccca taccaacagg ctctctatca tttttttgtc ggcaattaat
    42601 cttactctgt aaactcctga cgcgatttca ttgcgcggta taattacttg tccgttttcg
    42661 agagattgcg tttcatagat aatttgtccg gataaattac acaattcaat tctacccgaa
    42721 ttgtaattat tttcgaaggc aatcattaca taatttttag ccggattggg gaacacaagc
    42781 aaggatgcag gaacagactc ttcgatggca ataacaccgt cgcaatcagg gtcataaata
    42841 gttcccggcg aaccttcggg gcaaccggcg aaccaattat cgccattgtt catgatgcct
    42901 gaagcacttt caagttcaag tgtttttcct gcaccatcgg cttcatatgg ccatggcgca
    42961 gcatcatcgt aaataacggt aaactgcaaa ataccatgat cattaaacaa cctgatctct
    43021 tcacctgaag catctaatcc aaaattaaat gcgccggtaa catttgtaat atcaggatgg
    43081 cgtgattcga aaagatcgag gtcgccgctc aataccaatc tatcgtttgc cggaattgta
    43141 gtgcctgatt caaatgtata aataagagaa tcttcgccat caaggaattt ccatcctgaa
    43201 atatccatat catcagctgt agtattgagt atctccaccc aatcgcctgc attcattgct
    43261 gcggagctgt tgtaatttat ttcactaaaa acgagtgcat cattgcatgg cgaataagct
    43321 tctccgggtg aaccggcgat gcatccgtcg aaccaactac cgggattatt catatccgca
    43381 gtcgggttta ttaattccaa tgtgcgaccg gttccatcag caccttgtgg ccagccaagt
    43441 gaatcggcat atgccatgtg tatgatttca tttccggcga tgtcaaataa tctaatatcg
    43501 tcacctgaat tatcccattc aaaatcgtag ccgccaacaa cattcgttat atcaggatgt
    43561 acgtttgaga aggctatcgg atcttttgca attacaagat actcatccga ctcgattaca
    43621 gaacccgcag gcaagatata ggcattaccc tcatatcgat cggtgatttt ccagtaagaa
    43681 atatcaattg catcagttcc cgcattatgc aattctatcc agtcgcccat attataatca
    43741 acatagctat tataatttat ttcagataca ataatttcgg gagttacagc cgttcctgta
    43801 aatactgcgg taaatgttgt gctgttatcc atatttatgg tcaacgatat atcagtggaa
    43861 ccacccggaa gcaaggcatt cggcaaccac gaatcaaatg tgtagccggg atttggtaca
    43921 gcagtaattg taattggcac accatcgaaa taaatgccgg tccatggcaa ttctgttggc
    43981 acgatggtat ttattttaat atatccggca ccggcaggac tcacggccaa agtagttgtg
    44041 cgctgaccat cgaggtcgaa actattattg atatgtgttc ttgctgcatt tttgcgggaa
    44101 ttgttgtagc tcaacaggcc atctacatag ctatttactg ttgccgtatt tggagcgccc
    44161 catcgctcaa cctgatgtgg tatttcagaa agaatagaat cgcgcatttc gtacaatact
    44221 gcttccatat tatcaggttg ataaatcgtg ttgatcaaat cggcaaatcg attcacaaaa
    44281 tattcacgga tctcctgatt atcgagcaca cgatcaaata tctgagaata tgaagatgga
    44341 tatgccggac tgcgggtaag gttgatataa ttatcataag gtgaagagcc aaagtatccc
    44401 atagcaccat ccatatcata catgataaat cgccatttcc cgccatacct gtttcgccac
    44461 accttcatat tattcgcccc ccaataatat ccaccaaaat ctacattctg ataataaatt
    44521 tcacctatgt agtaatcgat ataattctgc atgtcaaatg cctgattgaa cttatcgtag
    44581 aaaatatcag acaatggatc taacgacatg agactggaat aaatattcca ccaaccggtg
    44641 tcgcttccat tcaacacaga atatgagttc aacaaatcga catcattatt attgatacca
    44701 taattcgatt caacaaaatg ttcatcagct ttttcgcgga tctcgtataa tccccatgca
    44761 tcgccattta taaacaacat acatggttgc cactcttcat aatcaatatg tgtatcctcc
    44821 atcacctttg ccagaaacgc atcctgaatt ttatatgtcc acacgtgctg gcctccactg
    44881 cgtatattaa aattatttat ggagcttgca tcaggcttac cgccgaataa tggatactcc
    44941 agatttcctc cgtacttatt tttaaaatca accctaaaac cgcgctgagc ctctgcccta
    45001 ctccagccgc cgtgaatttc cagcgacata tcttttcccc attcaaaatc tccatcagca
    45061 gtaaaatatt ctatatgtcc gaagcgctcc catggttgcc agaaattagc cccgaaatat
    45121 ggatatgctg catcgtaaca acatccgaat tcataaatac cggtatcata gtcaaataaa
    45181 cttccggggt ctatcgagat ggataatatt ggcaaggtgt agtcatcttc atcgataaaa
    45241 taggagcgtt taacttgctc tccggttaat aatgttccgg tactataaca tcgcgcagca
    45301 accacttcat ttgaagaaat tgttattgga ccggtgtata ccggcgatgt tgccgtaaca
    45361 agcgaaccat cagtagtata tcgaatttga gctgtcggtg aagtagacga aagtgttaat
    45421 gtttgtgttc ccgtataaaa tccggcagcg agcgaaaaaa ccggcagcgg ttcgtatccg
    45481 gcaaaacaag gtgcgccatc atttgaatta tcgggtgtgg gtgtggtagt tgtgcaccaa
    45541 cttgcagcac catcgggaat gcgcgcatag gcatgaccat aagttacgct tcccggattt
    45601 accatatctg caattgcacc ggtattgtcg aataaataaa ttgtttcgcc ggtattggtg
    45661 attttaaaat tcgtttcact gtatactacc ggttctgcaa accaggcggg cacgggtgaa
    45721 aaatatgtga gtccgttatt gatgccaaca gaaaaccacg catttgatga aaggtcagat
    45781 gagcctgcag taacgttatg tacctggatg cttaaaatat tttcaccggg caccaataat
    45841 atattcataa ccactgcagg aatattccat tcttcagccg gatatccggc atacatcccc
    45901 gcttcatgat cggcatttgc taaagtattg taagtcggag gtgttccaat aatgttaccc
    45961 gaccttgcaa tctccacgcc gttcaaataa gcaacaaatg catcgtcgta atccatatgc
    46021 aattttgcat tttcaattac acttacatct gccacatcaa atgcacaacg catatagcac
    46081 gaggtggcag aagctattac agtattatca tctccatcac caaaaccaat gcctccgggt
    46141 ccggtgctcc agcctgtatc gtcaaatccg acagtattcc aatcggaagg cggttcactt
    46201 gtgccaacaa aatatgttcg ctcagtttca tttaatactg ccgattccca atggtcgata
    46261 agcgcataag tattatttcc atttgccagt aacaataaat gctcattagc atcaagcatg
    46321 atggcgggca aaggccattt attcggaaag agcggatcat cggtcagcgc atatccgtta
    46381 aggtcgatgg cagtagctga attattgtac aattccagcc aatcgctttc ttcgttataa
    46441 gcatctaaga ctgctccgtt tgttgcaatt tcattgatga caagttgcgc cttcgcgaag
    46501 caaaatgagc ctagaagccc aatgaggagt atggtgcgtg tgaacatgaa taaactaaac
    46561 ttaaaattaa agttaagcaa ttaggctcaa cccaaatcta ctttccggga gagtttagct
    46621 taaaaaccga ttaattacat gcaatagcgg ttacctttgt cggacctaga tatgtcattc
    46681 ccaccgggat ttatagcaaa cttacaacac aatatcggtt tcgataaagc cgcttttgtg
    46741 gcgtcacatg acgaggtggc ccctgtatcg ataaggtata atccattgaa atcggcaaaa
    46801 cgcctgagcg atgaccccgt gccatggtgc gaaaacgcat gttacctggc ggaaagaccc
    46861 ttatttgttt acgatccgtt gtttcatgca ggagcgtatt atgtgcagga agcttccagc
    46921 atgtttttag atttcatact tagaaaactt gatgcagata aaaagccctt gcgcatatta
    46981 gatttatgtg ctgcaccggg aggaaaaagc actttgatag ccggagctat gcatgaagaa
    47041 agtattttat tcagcaatga ggtaattaaa acccgcgcgc cgattttaag agaaaatctg
    47101 acaaagtggg gaaaggaaaa tgtggttgtt ttaagtcagg atccggcgac tttcacgggc
    47161 atgcaggaat acttcgatat cattgttgtt gatgcaccat gcagcggatc cggattattc
    47221 aggaaggaac ctgcagcaat agcagaatgg agcgaagaaa atgtaattac ttgcagcaaa
    47281 cgtcaacaac gaattctcac agatatttta cctgcactaa aacccggagg atttctcatt
    47341 tacgcaacat gctcatatag tatggaggaa gacgaacatg tagtttcgca ttttattgaa
    47401 caaggtttga caaacagaaa tgatattatt tcatcaattg aaacacaagg tattatttca
    47461 acagcattag gatatagatt ttatccggac aaaattcgtg gtgaaggatt ttttatcagc
    47521 atcttacaaa aaaacaacgc tgaagaatta gcaatttcca aaggcaaaca actaaattat
    47581 cagctagctt ctagtgaaga gcgtgaaatc attcgcccat ggctgaagaa tttcgataaa
    47641 ttcattttcg ttaagcacaa caatgtattt tttgcctgga cgaaacaact gcaagaagcc
    47701 ttaaaagccg catcaggact aaatattctg caggcaggta tagcaatcgg agaaattaag
    47761 caaggtagtc tgatcccaca tcatgcattg gcgatgagca atatcatttc agacaatatt
    47821 ccacgcatcg ccctatcaaa atcagaggca ttagaatact taaggaaaaa taatattgaa
    47881 ttaaactcat cagaacccgg atggcatatt attacctatg aaaatattgc attaggttgg
    47941 gtgaagatga ttccgggaag acataataat tactatccaa ccgaatggcg attgagaaaa
    48001 taattactcc gcctcaggtt aatcgcttaa tgtaaatttt ataggaagtt tgtaaaatac
    48061 tgctgccggc ttaccatttt gcatgccagg tttccatttt ggcatagttg acaatatgcg
    48121 taccacttcc tcgccacaac cccctcctat gtcgcgcaat atagcaatat taattataga
    48181 accatcctct gtaactacaa attttgcgta gacattacct tcaattttct tttttttggc
    48241 agcctcagga tattgaagat ttgtattaat aaattcatag agtgccattg tgcctcccgg
    48301 gaattccggc attgtttcta aatattcata gatcatatta ggtgtatgat cggtggtatc
    48361 cattacaggt aaggagtctt gtcgccctgc ttccggtgtt ccattttgtg cggtggctat
    48421 tagtggtaaa acaagaaaag cgagagctgc gatcgggaat ctgagggtgt ttttcattct
    48481 aaagatatta tcccaaatgt aaccatcatc ggctaattca acaattttcc ctaaattgcg
    48541 tccacttaat ttacatcata ctatgaaaaa aatttactca ttgatgttgg gtgtggtagc
    48601 atggggtgca gtgttggcac agcccgtgac tattggaacc accacctacg atctacaaac
    48661 aaacagcgcc aataaaagcc gcctccatgt atatgacgat ggcaaggttt cagcgctatg
    48721 gacaggctca acaagcctgg cagccacttt ctccgacagg ggcatgttct ttaataaata
    48781 caacggcact acatggggtg gttttccaac tgctcgaatt gaaggcgtaa gaacaggatt
    48841 tggagaaatt attaatgtag gcgaccatga gat
//
LOCUS       DAOQOU010000005        42736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_358_length_42736_cov_5.800356, whole genome shotgun sequence.
ACCESSION   DAOQOU010000005 DAOQOU010000000
VERSION     DAOQOU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 42736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42736
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_358_length_42736_cov_5.800356"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3390
                     /locus_tag="PLM90_00850"
     CDS             <1..3390
                     /locus_tag="PLM90_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMZ87939.1"
                     /translation="ETDSLNIFTSEFIYNGGGIAIGDFNGDQLEDLYFTGNQVGNALY
                     LNRGQLHFEDITEKAGVQKKAGQWSAGVNVVDLNADGKKDIYVCNTFVNDPEKRRNLL
                     YVNNGDGTFTEKAKEYGIDDDSYSTHANFFDADNDGDLDLYVVTHPTDFKDKNKQKNF
                     QKIEEGTNMSNRFYRNEGNGKFTECHKEVGINNHGFGLSVTTGDINDDGWMDVFVGND
                     YIMHDYTYLNQGNGTFKESSRDVLMKTAYYGMGTDLADYNNDMLLDMFTVDMDIEGNY
                     GTKTFMQSNKQTFLRTLVNGGYLQQYGRNALQLNNGVGKFSEVANFAGVSTTGWSWSP
                     LFADYDNDGYKDLYVSNGFLKDSHMDIMEVYVKLTRANRLSDSTEYYELRKQIPESSV
                     LEWPNAMFHNNGDLTFTDVREDWGLYFPSTSYGAAYADFDNDGDVDIVVSNANMEAFI
                     ARNNSSKLNTNKYLRVDLKGEKGNPEGLGAKVYVTTSTGTQMVQMETVKGYLSCSEPV
                     AHFGIGSAEKVDQLVVRWLDGKENVLTNVAANQTVVVDHSTAMKPIKSAVPKPDALFV
                     NATEDLKVDFVHKENEFDDFDREFLIPKELSNLGPGVAVGDINGDGMDDFFVGGAKNQ
                     SGAIYTQTANGTFAKTSFAEGTEDIQSEDMGAIFFDADGDNDNDLYVVSGGSDFPKDD
                     VHLQDRLYINDGNGKFSWAKDALPKMISSGSVVAAQDFDKDGDLDLFVGTRIIPGNYP
                     YPTKSYILQNNKGKFTDITSSVAPDLEKIGLVSAAIWTDVNNDGDYDLMLTGEWMPIT
                     IFENNGGKFKNITNAAGLSESTGWWNSLTAGDFDNDGDMDYVAGNFGTNLKYRPKEKP
                     IELFYDDYDNNGTHDIIMGYWQHDKLYPEKTRERMIEQIKDVETIFPDWDSYGRAEVW
                     DFYGKKRMENSQLHYKANSFYTSYIENQGNNKFAVKRLPNDIQISSTFGIVPMDVNQD
                     GNLDIVAHGNFYETEIETNTQDAGIGNVLLGNGDGTFTPLHARYSGFYSAMNAKSLAM
                     IKVGAAKTPVLLTTNSNNKMEAFKLAKNTAVGVNANDAYAVITFSDGKQRKQEFYNGA
                     GYLSQSARAVVVNDAVSQVVVYDNKGTARTIFGQQVSMNKK"
     gene            3418..7038
                     /locus_tag="PLM90_00855"
     CDS             3418..7038
                     /locus_tag="PLM90_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMZ87940.1"
                     /translation="MMKIHNVSVLIAAFAMFTVASCKLFSGGDVDTRGTAIIDPKREV
                     KLDGNNGKLFDILTDKSTGITFVNDLTENYSVNWWRYSYIYNGGAVCIGDVNNDDLPD
                     LFFTGNLVPSRLFINKGNLKFEDYTAKAGITSNKDKWPYGASMVDFDGDNDLDIYVCM
                     SRWDQPERRANEMWVNNGDGTFTEKAKDLGLTDNSYSTHANFFDYDNDGDLDIYLVTH
                     PVDFIDKNKTKYFQKIEQGKNLSNHFYINNGDGTYTESHLKVGINNHGYGLSASVGDV
                     NDDGYQDVFVSNDYAMYDYLYINNGDGTFRDESLTAIKKTSINSMGTDISDFNNDGFL
                     DMFVVDMDMEDNYTYKTFMLSSQVEVMRILINAGYGYQNRSNSLQLNNGDGTFSEISR
                     TANVSTTDWSWTTFFTDFDNDGWKDLFIANGFLRDFHVDESETYHKLRRAVRIEDSTV
                     YYEVRKELPNYVLDHPNYIYRNNGDLTFEDKRDDWGIYYPSITYGGGYADLDRDGDID
                     MITSNVNQSPWVYRNNSEKMNTGNNYINFKVGGYAKNTAGLGTKVRIFTGDNMQYIQY
                     TASRGYITTIIDNIHFGVGKATKIDRVEVEWLDGTKLMLTDVPVNQVVKLDHAQASKS
                     VQFPVRVKGDKIFANNTESLGTDFEHKEADYDDFLREFMLPHKMSTLGSGIAVGDVNG
                     DGTDDFYIGGAAGQSGALYTQNENGKFTRSSWTQGMNDGLASEDAGALFFDADGDGDN
                     DLYVASGSNEFASNDKRYADRFYRNDGKGNFTYDSSVLPAINSSSSSVSGCDFDKDGD
                     IDLFIGGRQVPGRYLAPASSYILKNDGGKFSDATAQVAPMLKDIGMVTSGLWTDFNND
                     NNIDLILTGDWMSITFLKNSGGKFTDVTASTGLNNATGWWQSITGADLDNDGDMDYVV
                     GNFGTNRRYKNTVSSQGKPLPLEAFLYDFDKSGQEDYIMCYYQHDKLFPVKTRERMIE
                     QIPSFEEKFPTWNEYGIATVQDMFGKDLDNAIHRTAYIFHSSVLMNDGNGKFTMKFLP
                     NEAQISTMFGTIIDDFNEDGIQDILMHGNFYNTEIEITRHDAGTGLLLIGKGDGTFTP
                     NRSYINGFKSDGDAKALATLLDSKTKQPVILAGNSQGKMLAFSLVKPTKTIELAAADA
                     YAIVTRTDGKTTRVELYSGSGYLSQSAKSIRITPLVKQVDVYDYKGNMRTVYPGGNLA
                     SK"
     gene            complement(7117..7755)
                     /locus_tag="PLM90_00860"
     CDS             complement(7117..7755)
                     /locus_tag="PLM90_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HMZ87941.1"
                     /translation="MQKFLPIFPLNLVAFPGEQLNLHIFEPRYKQLISECSKENKTFG
                     IPVVDNNQMLDHGTEMELIKIHKVYPNDEMDIQVRGLQVFRLLEVVREIPDKLYAGAI
                     ISLLPNVEDKHSRLALELEELTVELFSLLDVREQMEKPDFAFKSFRLGHYVGFDLKDE
                     YELLRHVRETERQKLIVEHIKRILPSVKQVSDMRERAKLNGQYRMVNPPDFL"
     gene            complement(7764..8387)
                     /locus_tag="PLM90_00865"
     CDS             complement(7764..8387)
                     /locus_tag="PLM90_00865"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HMZ87942.1"
                     /translation="MGSRFYFSLMGCVLILFASSCRQKQTTLPDPHLSYAQVIQKQRN
                     DQQAYFAGEGSPLSDSARANFKGLQFYPIDSSYRVSGNFSPIANGPIFTMQATGDVAD
                     IYQIAGNIQFKLKDTICMLEVYRNVTYYQVEHKESYFVPFYDKTNGQETYEGGRYLDV
                     HKLQPGAMQLDFNLAYQPYCFYSHNYSCPIPPRVNSLPVYVRAGERK"
     gene            8440..8811
                     /locus_tag="PLM90_00870"
     CDS             8440..8811
                     /locus_tag="PLM90_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HMZ87943.1"
                     /translation="MASLFDIEAFRDYCLSKKGVTEEFPFGPDTLVYKVMGKMFALSG
                     LDEIGFSMNLKYNTEHIDELRDKYPAVQPGYHMHKRHWNTVMVDGSIPVKELKSLIDH
                     SYSLVVDSLPKKLQAELNALK"
     gene            8812..9507
                     /locus_tag="PLM90_00875"
     CDS             8812..9507
                     /locus_tag="PLM90_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HMZ87944.1"
                     /translation="MISEDQILYIDNHLIAINKKPGEISQSDVTGDVSLLEDVKLFIK
                     EKYNKPGNVYVGLVHRIDRPVSGVLLFARTSKAAERLSQMIRDRAFKKTYWAVVKQMP
                     KEPEGRLENYLLKNAQMNKSFVAKNEKGGAKKAVLHYRLIYSLERYHLLEVILETGRH
                     HQIRAQLSAIGCPIKGDLKYGFDRSNPDGSIHLHARSISFEHPVTHAAITIIAPPPQD
                     VIWNACLKVAEQQ"
     gene            complement(9555..10394)
                     /locus_tag="PLM90_00880"
     CDS             complement(9555..10394)
                     /locus_tag="PLM90_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ87945.1"
                     /translation="MKKATLLIIAFLGIAGVASAQQGLHFGFHAQMNSIWIINQNNYE
                     YSQMDYEYKYGKLGGMSLGYNWDDNFGWQVELNYSQMGQDYSDIIKDFGPITDSTKPD
                     LRTKVLTYRYVDLTYLQVPVLFKYMEGDSKDAIKYQMLGGLQFGYLLGADQQYTADLY
                     DLDPDDQKPVEQAGFHAPFDAVPDFSGNAGSDGTEFFTKLDVGALIGIGADIYVNDKL
                     YFTPSFRGYFGLFDINAKETRELRPAQGENRYLASHNAFVGFHLGISFMFPDARLGGG
                     SDE"
     gene            10653..11441
                     /locus_tag="PLM90_00885"
     CDS             10653..11441
                     /locus_tag="PLM90_00885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ87946.1"
                     /translation="MFNVHMAGQFLRYQVRALSRYELHSPFLFDLEEKVLHDKRHFYA
                     FDEIEYRRELLLAEDQTISVSDFGAGSHVHKSPERRVSDIAKTSLITPHFGQMLYRLV
                     QYLQPVTLLEMGTSLGISSLYMAKAVPGAQLITLEGSSAIAQIARKQHIALDAKNITI
                     MEGEFNATLPSALNALKKIDLAFIDGNHRFEPTVAYFESIYPYLHEHSILVFDDIHWS
                     TGMDKAWHVIKSDARVTCSIDLFYKGIIAFRPEFRQPQHIVLRY"
     gene            11524..12897
                     /locus_tag="PLM90_00890"
     CDS             11524..12897
                     /locus_tag="PLM90_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ87947.1"
                     /translation="MRKRMMISMCLLLLAGIMHAQSPLPSNFFLKTLPNGLQMLVIED
                     PTVPLATIEIVVKNGSYTEDSTFNGLSHMYEHMFFKANKSLPSQEAFLEKVQELGVVF
                     NGTTSNERVNYFITLSNAKLGEGLVFMNNAIRYPLFLEQEMKNENPVVDGEFQRNESN
                     PVFFLFDDLNRHMWKSNYYRKNAIGLHDVILTCTPEKMRIIQEKYYYPDNSLIAIAGD
                     VKHDDVFAQVEKIYGDWKPGGFDPFQKWPIPEFEPLKGVENFVTINENAQIPMFITGY
                     HGPDTRNDVKATYAADVFSYLLNLSDSKLRQELVETGLAYDVNVGYQTCKYTGPINIF
                     LVPNPAKVSEAWKTLQENIGQWASPNYFTDEQLETAKNQLAIQDAYDKESISNYVHTV
                     TYWWASASLDYNTNYIKNVQAVTREDIQRYVETYIIDKPHVNGLLLHPDMLQMVDVKA
                     LGFEVKQ"
     gene            12925..14253
                     /locus_tag="PLM90_00895"
     CDS             12925..14253
                     /locus_tag="PLM90_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ87948.1"
                     /translation="MKKIQITIVLLVTLLSANRLTAQDTKEYMVDGIKVIHKQVPKDV
                     ISVQLFIEGGTANYTKDKQGIENLTLNLMMDGGTTGMSKLEFKSAAEKIGTTFNAGSN
                     IDAGTLSMNCIKLFWDDSWKLFSDAILHPAFGEAEYNLLREQLIAGAQQSMADPDTYL
                     ALLSRQVAFENKDYAKAPEGTPETLTGITLDDVKTYYKKTIGKKRVFLVVVGNISQED
                     LTAKIHNTLAALPGGSPASSESRTLITQPKTYIEDRDIATNYIRGIMSAPFANDAEGI
                     AYRVAISIVGDRYFTELRTKRSLSYAPAAFYANAGITNPYGVIYITTIDPKQSMQVMV
                     DELNKIKKEGFTEKELRDKQQEFLTTYYLTLETTANQSNALGMAEMSGGWENLDKITA
                     AVNSLKLEDINAVFDKYSNAIVWTYLGKKSAISEADFPQTTPKEKKNKPY"
     gene            complement(14322..14837)
                     /locus_tag="PLM90_00900"
     CDS             complement(14322..14837)
                     /locus_tag="PLM90_00900"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ87949.1"
                     /translation="MASKRVSESATTFTEIVMPNDTNPMHNLMGGNLLKWMDIACGIC
                     ASKHANRVAVTAAVDNVSFGRPIKIGDIVTISARMTRAFNSSMEIFVEVFKENFRTHE
                     KVKCNEAYLTFVALDDDGRPVSVPEIEPENDDEKRRFVSALRRRELRLILAGRMKPEE
                     AKELKQIFLNE"
     gene            14997..15860
                     /gene="ygiD"
                     /locus_tag="PLM90_00905"
     CDS             14997..15860
                     /gene="ygiD"
                     /locus_tag="PLM90_00905"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HMZ87950.1"
                     /translation="MERRKFIKAGTFSLLAMQLKEFSNTFQQEQDIVMPAMFVGHGNP
                     MNAIEDNEFSRGWEAQGKLLPAPKAILCISAHWLTRGTRVAAMEKPETIHDFGGFPQT
                     LFDVQYPAPGSPEFAKETSTVITKTKVELDFEWGLDHGCWSVLNKMFPNADIPVYQLS
                     IDYYQTPAYHYALAEELKSLRKKGVLIMGSGNIVHNLGMVQWADTAYDWAIEFDTKIK
                     EFIDARNHQGVINYKDLGKSALLSVPTNDHFLPLIYTLGVTDTKEAISYFNEKNTMGA
                     VSMRSFVAGLS"
     gene            complement(15914..16225)
                     /locus_tag="PLM90_00910"
     CDS             complement(15914..16225)
                     /locus_tag="PLM90_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87951.1"
                     /translation="MKRCIIILFVIAAMPVFGQSARISPAAFTSLSVGFVQHTEPVPH
                     GGQGIAATTVNPNPNVGTSNIVVQEELLAGRKSNPGLREKITSRERNTNKQPLFKKKR
                     H"
     gene            16312..17622
                     /gene="sucC"
                     /locus_tag="PLM90_00915"
     CDS             16312..17622
                     /gene="sucC"
                     /locus_tag="PLM90_00915"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMZ87952.1"
                     /translation="MLITELAVNSCDVLITNTLAYFNRNEAKRIVYICPQNAKYMNLH
                     EYQGKELVKRFGVKIQEGIPVDTPEQAVEAARQLQAATGANIWVVKAQIHAGGRGKAG
                     GVKVAKSLDEVYEKASKILGATLITPQTGANGKKVHKVLVAADVYYPGASDTKEFYMS
                     ILLDREKSRNVIIYSTEGGMDIEEVAEHHPDKLHKEWVDPTVGIQAFQARKIAFNLGL
                     SGAAFKDMVKFVTSLYNAYVGNDCSLLEINPVLKTSDDQIIAVDTKCVVDGNALYRHA
                     DIMGMRDITEEDPTEVEAGEAGLNFVKLDGNVGCMVNGAGLAMATMDIIKASGGNPAN
                     FLDVGGTADAKRVETAFKIILKDPNVKAILVNIFGGIVRCDRVAQGVIDAYKNIGNIP
                     VPIIVRLQGTNAEEAKKMIDDSGLQVRSAILLKEAAALVKEAVL"
     gene            complement(17991..18257)
                     /locus_tag="PLM90_00920"
     CDS             complement(17991..18257)
                     /locus_tag="PLM90_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMZ87953.1"
                     /translation="MVITFEELRHIKDALPHGSMERIAEELHIEVDAVRNYFGAEHFE
                     APGKIADVHFEKGISGGVVRLEDTAILQSAMRILDEAKHVQTLN"
     gene            18502..23898
                     /locus_tag="PLM90_00925"
     CDS             18502..23898
                     /locus_tag="PLM90_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HMZ87954.1"
                     /translation="MRAKLIWPGLLALGLYACNADRNTIAIKTGDFPETIELRQNLEI
                     HLNQHVFNDSLFDKWVDADLLQIDPPVEGRFKIVSSDAFYFSPSKGFAESEAYTATLG
                     KDLFNYTDLKLALKSAPVNFHTTLLDLTAVNTYWFLEPTSGEVKLNAGLQFTASVDPQ
                     TLGDMLTVKVDGTNKTSSITSTQSADNMAVTISGISPKAKDQDLNIDIRAGIGSPGSR
                     YKTPKPLHMKVPVAPVEDLEIRDITANHDGIVGTITVESNQQFSNTNLEELVKIEPSI
                     PVTIEKTLNGLVVTSEQFNASQTYVIILSDKLRGVAGGKMVDTYQQSVTFGELEPSIE
                     FTQQKARYLGGKGERNISVRIVNVPKVQVNVVKIYENNILAFLRDGFSWDWYESYDAE
                     EEYWDYHDYQNVNTSLYGNVVYTEEIETSKLEKYNSARVLHLDFTDKLERFDGIYVVT
                     VSAAEKVWLTDAQIVSISDIGLIAKATEDELVVFANSIQTAEPMSGIKVSFISSNNQK
                     LYTASTDKNGVARFTGLKQNMPDFNVNLITAKNGSDYNYLPFSETEVSTSRFDVGGKR
                     LNSSDIDAFLYPERDLYRPGETMHIAGILRTYDWQLLDRIPVKIKVISPSGKDFKTLR
                     KTIDDQGGFETGIDIPKNAVTGTYTVQLFSSNDILLASEYISIEEFMPDRIKVSGSLN
                     KQNYYPGESVQSTIVANNLYGTPAAERNYESEFNLQQHFFSSKKYPAYNFQISGGTTY
                     FPSQNAEGQTDENGQAVQSYTIDQTYSKVGLLDGRILNTVFDESGRPVYQVQNFTVIT
                     QSNLLGIGEFDYYVKVKSPLRIPMIAVDKDDKGYKTQDAHVEIIKYDYETVIEKHRNY
                     YSYRSNRKEKILQSYDKKISGDQDGIWFTPQQSGEYQIRLYLKDAKAYVEKSFYAYGW
                     GSTQNNSFEVNTEGNINIISDKPKYDLGEEAEFLFTTPFNGKLLVTVEREKVIEYHYL
                     DTEKKAASLKIKMNQSHVPNVFVTATLFRPNDESGMPLTVAHGIINVEVKNPAKAIPL
                     TVTHEDHSRANTKQTINIKTAPNAQVTVAVVDEGILQIKNFTTPDPYNWFYMQHALGV
                     FSYDLYAYLYPEIYLKKMLSGGDAMMNEGRLNPMTANRVKLVSYWSGILKADASGKIA
                     YTIDIPQFSGDLRVMVAAYRNDQFGASESHMKVSDPLVMTTSLPRFLTPGDTIDVPIT
                     LANTTDKKASGKVTMSIEGPIKVVGSSTQQFNANANKEARSVFKIVAWGATGNAKVKV
                     QSSALGETFTDITEIPVRPPAGLQKYSGEGYLDGNKSITVNMQPNLISSSAKARLMIS
                     YNPLVQFADDLDYLVQYPHGCVEQMTSSAFPQLYYADMVKSFYSKERPDLDPAYNVQE
                     TIRILQSMQMYNGALTYWPGGGYESWWGSIYAAHFLLEAKKAGYDVDQKTLDKLLGYL
                     RTMLKQKKTYVYYYDKGLSVEMAAQEVFYSMYVLAVSGKAEMSMMNYYKANLSMVPQD
                     GKYLLAMSYMLAGDKRSADKLLPGGFTDADPKQQFNGSFNSPIRDRAIALNCMLEADP
                     NNQQIGRLSQQLSEMVKNARYMNTQERAFTFLAFGKLAKQSKEHNAKVVIKIGGKEAG
                     TFDGTDLVIEDTKMASSPVTLNSSGSGRVYYFWYEEGVSADGSVKLEDNFLRVRKSFF
                     NRWGQPIDLKNIHQNDLVVVKISLVSATGTYVENIAITDILPAGFEIENDRLRATNSM
                     PWIQNQSEAQHEDIRDDRILLYTDATAKEKYFYYTVRAVTTGTFKMGPIMADAMYNEE
                     YHSYNGGGWVTIKK"
     gene            complement(24169..25728)
                     /locus_tag="PLM90_00930"
     CDS             complement(24169..25728)
                     /locus_tag="PLM90_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87955.1"
                     /translation="MKRLMLLSIFSVFSSLFVKAQSGDVNDLPPIDELGTGTFMGRQG
                     GLYPNGSNSMPAAFYADAMAMAQSVQPLDKSGKPDPKGRIGVIALGASTVAMFSKGLE
                     HQIPEAAGVNKELTFVNCGIGGQDLSDIMNPAANYWSVIDQRVREAGMDLNQIQVIWF
                     QEDNLRNRENNFDLRIAQLTKEFTYMVRFCKEHYPNVKLFYVSGRHTTDFMPADAKDK
                     HREPKAYINGWVCKQLIENQINGSEELAFKGANAVSPLILWGPYFWTQGEKPRKDGYT
                     LTRDMISNDGVHPTEKGIEKVSKDLVDFWRTDPVSQQWFLENPGEVTVDTIGDETAAD
                     MQTMAFIINSKTILNIPYDKIAATYKLTVLKDSAVVFNNDAAQVGPTLELDIAGAGNY
                     KYLISDGTNNYSGKVVVDDALQVTVVKDTVVSGKGKHPIDPNSPAWVVNGANKMAKLQ
                     HVLRPNTNVKVILTDDKGKEVLVVEDFLNKYTDVNEALDRGEYKMTFYNEKGSVISVP
                     EEFNSSIRVKY"
     gene            25883..26968
                     /locus_tag="PLM90_00935"
     CDS             25883..26968
                     /locus_tag="PLM90_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87956.1"
                     /translation="MLGYTDMQGDYYVSDSGRIVHLEHLRVKSSQPAANSIAYISNTG
                     NLVYYSNHEQVKLDITNPTFYKNTDHYLYYSTGGSFSVYNGKERKYLGYIQQYPYAFA
                     DSIAAVHDYSGYFYTYVNDLFIELENQPVLKVIAGDNILAYVNHIGQFKAYYQGNKVD
                     LDDYAPITAQAAGSIIAYIDNYQYMKVYYQGSIHELFNLPEINCLSTTSSGDPLLASW
                     CNGPVVTDVQSELPVFQVGDDMVAYIDDMGRFNVFYKGNIVALESQPPKQYTVVDRVL
                     YYTDENDYLKVFCNGELSIVETYTPSRIEADGEVLAYMDLDKRLKAFYLNTSVDVSEN
                     IVLDFSLNNTLLMYSEIPNKYKFYSLE"
     gene            27097..29310
                     /gene="pbpC"
                     /locus_tag="PLM90_00940"
     CDS             27097..29310
                     /gene="pbpC"
                     /locus_tag="PLM90_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HMZ87957.1"
                     /translation="MHVKIDYSQTVTAADGTVLHAFLTTDDKWRMMTELSEITPEMQK
                     AIIFKEDKYFELHPGVNPLAICRALFNNIRTGHRTSGASTITMQVARMLEPKDRTYGN
                     KVVEMFRALQLDMLYSKEEILQMYLNLVPYGGNIEGVKAASILYLQKMPAVLSLSELT
                     ALSIIPNRPTSLVPGQNNAQIIVERNKWLEKFRNADLFSDDIIDDALAEPFNAKRTEI
                     PQLARHFSTRMRMEFPADPIIKTTLQPQLQSQCEEVMSNYVGQLWALDINNGAAIVID
                     NKSHKVVAYVGSANFFNERDGGQVDGVQAVRSPGSTLKPLLYAMAFDKGIYTPQSIVS
                     DVPINYTGYRPENYDEDYHGNVSITYALANSLNVPAVKTLHEIGMEDFLNMLQRAGFS
                     SLTNQRKDLGLSVALGGCGITLEELTRAYSAFPNNGKSPHIRYLQSDNDTTATPVFTE
                     AAAFMTTEILSELTRPDLPLFYQNAKKVPRVAWKTGTSYGRRDAWSIGYNEQYTVGVW
                     VGNFSGRGVPELSGANVATPLLFNIFQVLGSDVDADWNRMPESVQFRYVCEVSGEIPT
                     ANCSHVVMDYFIPGISKNDSCTHLIDVMVDPKETISYCNTCKPETGYKVKQYQNYSPQ
                     IISWMQEKHLPFESIPPHNPKCERMFSDQMPSITSPADQMEYLIEKDDPQRIMLSANV
                     ASDVKTIFWYINDKLLTSVNAGEPVFFDPAEGKTKISCSDDKGRNVDIEIMVEFF"
     assembly_gap    29337..29436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29593..30981)
                     /locus_tag="PLM90_00945"
     CDS             complement(29593..30981)
                     /locus_tag="PLM90_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HMZ87958.1"
                     /translation="MKKIIMILALPAIIMAQGCSGWVDEVSTSPNSPTEVTPDLLLSV
                     SEVAMQTLYTGQLTRTASILTQQCAGNDFQMIDIRDYKITELSNTNEWKTVYADALIN
                     QQTLIDIAGDENPYYRGIARVLKCMTIGIATDFWGDVPYSQALQGLDGTDAALNPEYD
                     AQADIIAAMQSELDAAIADLSMPSTSNAFLPASDDFIHNGDVSMWIKAAHMMKARYAN
                     RLSKRDATGSANEALMHIAESGVTSNADNTNAIFGTNGNELNPWFSFNTTRANYIKMG
                     AFFIDLLSSTNDPRLPLYATTDDGGGYSGVATNSVDPAASNTGELYSSDASPLPLLTY
                     YEMKFIEAEANLRLGNNDAAAAALNDAVKANILATVGAPDPVYEAAHASETGASITLD
                     KIMTQKYIAMFTQPESWADWRRTNIPALTPNPDAIYDEIPRRFPTCIDERLYNSNVQV
                     VGDITLPVWWDE"
     gene            complement(31000..34173)
                     /locus_tag="PLM90_00950"
     CDS             complement(31000..34173)
                     /locus_tag="PLM90_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ87959.1"
                     /translation="MRKLAASVLLLMFGMVSVAFGQGKTITGTVTSATDNQPLPGATV
                     MVKGTTVGTVTDLDGKYVLVVPQDKDVLVFTFLGFKTLEESIGSKNVVNIAMDEDVLG
                     LETVVVTALGIPKEKLSLGVSTQEVGGDRLASSGEENLVEALSAKAAGIQVVGSAGTP
                     GASAKIIIRGPSTFTNENQPLIVIDGVPIDNSTTSTVAGDYPFNPTLNGVSNSNRAID
                     INPDDVESINILKGPAAAALYGSRAGSGAIIITTKRGNKAGDKAVHINFSSSLEVSQV
                     NKIPAQQQLYAQGSGGGFTDADGNVDPEGNYITSDPGPDFLWGTDDDGSAGTANSWGP
                     LMSNIGITPTDNPGEFFQNGMTYNNNLSVSGGTDRGSLRLSIGNTQDKGIVPNTQFDR
                     TSVRVNSDMRIYDKLKVAVDANYITSGGTRAQNGSNLSGVMLSLLRTPTSYDLGAGYE
                     YPSGDNRNYFSAYDNPYWTVNNNPYTDNVNRLLSNVSATYTPWTWLNVTYRVGADNYT
                     DLRKQIFSIGSNDPANAPGGQIEENTIRYRQIYSDLIATATHQVNEDLGITLTVGNNL
                     TDIDINNQYSRGRDLTIPDFYNLSNAADLYSSQYTTKQRNAAFFGDLEFDYRELLFLT
                     LTGRDEFSSTYGPNQSSAFFPSASVAFVFSDILTTNNIWSFGKLRFAYAQAGIEPQPY
                     SADTYFIPPLYTDGFTDGYSFPYLGQSGFGYSQLNTLGNPDLSPERLTGTEFGLELKF
                     WKGRIDLDAVYYIQESSDILLTKPIASTSGFSYVYDNAGAMTNKGIELTLNADIIKKK
                     NLTWNVGGNFSKNENEVTQLADGVDEIEIEAGFGDPGAYVIVGQPYGLLYGSQWAYNE
                     DGDLLIDPSSGLPIFDTVSGVIGDPYPDWLMNISTALTYKGFTVSGLLDIRKGGDIWC
                     GTIARMNRIGISEASGDRTETYVIDGVIENADGTYSQNDIAISPADYWQSYQGDFGAS
                     SQAVYDGGWVRLREVKLAYEFNLDKKNVIKGLTVAATGRNLFLKTDYPGVDPETSLTG
                     AGSNISGFDWFNNPGTKSYLFTVSAAF"
     gene            complement(34351..35076)
                     /locus_tag="PLM90_00955"
     CDS             complement(34351..35076)
                     /locus_tag="PLM90_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMZ87960.1"
                     /translation="MFRNFCLAAITMYLFTGATCDPQVMEDVLNTVMSTTITDQEASN
                     GLIEALVQGASNGSATLAKVDGYLKNPSVKIPFPPEAQKIETTLRDLGLNKMCDDVIN
                     SVNHAAEDAAAEAKPILVNSIRSMTFSDAMNILMGADDAATEYLKKTTTAQLTAKFKP
                     VIDKSLSEVGATKYWSDAVNYYNKIPLVEDMNPNLSDYVTQKALDGLFYMIEQEELKI
                     RQDPAARASEIVQKVFGYYDTHK"
     gene            35211..36467
                     /locus_tag="PLM90_00960"
     CDS             35211..36467
                     /locus_tag="PLM90_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HMZ87961.1"
                     /translation="MRAALISIGDEILNGTTINTNASWISTVLQPLGVDIHEVLAISD
                     HADHILGTLHAYVGEMEIIIITGGLGPTKDDITKKTLCRYFDSTLEFHQDIYDRLKAA
                     FAKRNIPFTENNRDQAMYPHNCEVLHNALGTAQGMLFKRNGSVIVSLPGVPFEMKGLM
                     TDQVLPRLREELQFPTLLNQYFMTSGISESFLARQLEPLEAMLPGHIGLAYLPSPGVV
                     KLRLTARGSDAATLKAELEMLSNEMRRILGSGIYAEEPISIEEHLGKLLVEGKATLGC
                     AESCTGGKIAHKITAIPGSSNWFKGGVIAYDNQLKINILQVNESIISQFGAVSEETVS
                     AMLDGALTVLGTDFAIATSGIAGPTGGTADKPVGTVWVGVAGGGKKVVHKYFFNKNRE
                     INIEYACMFALHEMRLLLTEILVKPS"
     gene            36554..37855
                     /locus_tag="PLM90_00965"
     CDS             36554..37855
                     /locus_tag="PLM90_00965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HMZ87962.1"
                     /translation="MAQKELIMPKMGESIMEATILKWLKKEGDTVSVDEAVLEIATDK
                     VDSEVPSPFAGTLTKILFKEGDVVQIGKAIAILDGDGSVTTKTAAPEPAVKTEVVVET
                     PKVVAPVVQTEVPVVAGDRFYSPLVLNIAREEGISMAELERIPGTGKDNRVTKGDILN
                     YVQNRGAGTTQAPVEQQAPVTQTKTEEPVIVKTASVSGNVEIREMDRMRKLIADHMVM
                     SKRVSPHVTSYVEVDMTPIVEWRNRIKGKFEQREGQKITYTPIFIEAIAKAIKDFPGI
                     NISVDGNNIIYKKDINIGMAAALPSGNLIVPVIKNADRMNMVGLTMVVNDLANRARAN
                     KLKPEEIQDGTFTLTNVGSFGSLMGTPIINQPQVAIMAVGAIKKRPVVLETTQGDVIA
                     IRHMMFMSMSYDHRVVDGMLGGSFLKRVADYLEGFDTEREL"
     gene            37889..39280
                     /locus_tag="PLM90_00970"
     CDS             37889..39280
                     /locus_tag="PLM90_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ87963.1"
                     /translation="MYIEQLYTNCLAEAAYYIESNGEAAIIDPLRDTAPYIEKAASRG
                     AKIKYVFETHFHADFVSGHIDLARQTGAQIVYGPEANTSYDVVNAADGEIFHVGSLQI
                     RALHTPGHTLESTCYLLIDANGKDHCIFTGDTLFVGDVGRPDLLDGIMTKEELASKMY
                     DSLNNKIKPLADDVVVYPAHGPGSACGKSMGKETFSTIGIQKQTNYALQPMTRDEFIA
                     AVTDGLSQPPKYFFEDARINKQGYEDVEMVMAKNAKPLSITAFETAVESGALILDTRI
                     ADIFEKAFIPNALNIGLNGMFAVWVGTLVDIDQPLVLVTEPGKESESVLRLARVGYEN
                     VLGFLEGGIDAWIQSGRTIETVTTIDPGSIKPYMGNDDYIVLDVRKPSEFENEHISGA
                     INIQLSEIGARLHELQPNKQYIMHCAAGYRSMMAASILKANGLNNFVNVRNGWTGIKK
                     TDLPTESSVPELN"
     gene            39300..39698
                     /locus_tag="PLM90_00975"
     CDS             39300..39698
                     /locus_tag="PLM90_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87964.1"
                     /translation="MRKLILIIAVIISFTSCDNFKESEAIEHITRTWTVYKYEVDGYD
                     QTPLFVATYGTYTLVCEKDQDYTETYTVGATPVTITGIYIFSDGDNILTLSDEFQSRV
                     FNVLTLTNNEMTLEDRNADTNQVYYLEAVI"
     gene            complement(39750..40391)
                     /locus_tag="PLM90_00980"
     CDS             complement(39750..40391)
                     /locus_tag="PLM90_00980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ87965.1"
                     /translation="MKKTSVLLLLAVLMHSAVFGQSDRKKYKHLPDGLYAEVQTTKGN
                     ILLFLEFQKAPLTVANFVGLAEGKIENTAKPLGTPYYDGLTFHRVIADFMIQGGDPSG
                     NGSGGPGYAFKDEFHSDLRHTGPGILSMANAGKFTNGSQFFITHKETPWLDNKHAVFG
                     HVVEGQDVVNAIQQGDKINHIKIIRKGKAVKKYDPAAVFKSFEDKGDVKRQGK"
     gene            complement(40407..41456)
                     /locus_tag="PLM90_00985"
     CDS             complement(40407..41456)
                     /locus_tag="PLM90_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMZ87966.1"
                     /translation="MVTLVPELTMELTNLFNWLKQPRRITLLTHVRPDGDAIGSALAM
                     KMYLEKKGHTANVIAPDDFPDFLDWMPGSKQVYIHQRNRKTCFAILTQSDAIFFLDFN
                     AIKRIEALGEDVMKLTRPYVSVMIDHHREPDNFTDYRLWNDHASSTAELVYTFMEMAG
                     DIHLLDQDMAACIYAGIALDTGIFQYSNTTAHVHAIAGKLMEYGIKVDRIHNDLYNQY
                     GENRIRFIGYLLSQKLQIIPAYRAAYMSITMAEAEQYKLSIGDKEGIVNLPLAMKDID
                     IAVLFTEDKGKVKMSLRSKGDVNVEILARNFFNGGGHMNASGGSSKQSLEDTIKTFEE
                     VLPKFYAMPEANTNQ"
     gene            41520..41939
                     /locus_tag="PLM90_00990"
     CDS             41520..41939
                     /locus_tag="PLM90_00990"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMZ87967.1"
                     /translation="MAGNITLTMIKPDAVKKNYIGPILKMINEAGFRIVAMKYTKLSK
                     EKAGEFYAVHKERPFYGELVDFMSSGPIVAAILEKENAVEDFRKLIGATDPSKADEGT
                     IRKLYAASIGENAVHGSDSDENAAIEGDFHFSKLERF"
     gene            complement(42141..>42736)
                     /locus_tag="PLM90_00995"
     CDS             complement(42141..>42736)
                     /locus_tag="PLM90_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ87968.1"
                     /translation="FSNWKLLPRDKNDIDGYHTTYPNSIVLFNFTTPAASGAIDQAAG
                     TITLTVPAGTDVTALVPTIDYTGQYIDPVGGVAQNFTSPVNYTCFSPVDYTPKTYVVT
                     VNFGVSATALAMAEMTVSPNPATDEMTVEIDMPSASDITFDLLDLEGRKVLTTSELCT
                     VGKNIFRFDLRGLSNGLYLLQLSSSTETTIRKIEVSR"
BASE COUNT        12942 a         8668 c         8935 g        12091 t
ORIGIN      
        1 gaaacagact ccctcaacat ttttacttcc gaatttattt acaacggcgg cggtattgcc
       61 atcggagatt tcaatggaga ccagctggaa gatttgtact ttaccggaaa tcaggtcgga
      121 aatgcgcttt acctcaaccg gggccaattg cattttgaag acattaccga aaaagcaggc
      181 gtacaaaaaa aagcaggcca atggtcggcc ggcgtcaatg tagtagacct caatgccgat
      241 ggcaaaaaag atatttacgt ttgcaatacc tttgtaaacg atcctgaaaa acgccgcaac
      301 ctgttatatg taaataacgg cgatggcact tttactgaaa aagcaaaaga atacggtatc
      361 gacgatgata gctattcaac gcatgccaac ttcttcgatg ccgataacga cggagatctt
      421 gatttgtatg tagtaaccca ccctaccgat ttcaaagaca aaaacaaaca aaagaacttc
      481 cagaaaatag aagaaggtac caacatgagt aaccgcttct atcgcaatga aggaaatggc
      541 aaattcacag aatgccataa agaagtaggt atcaataacc acggattcgg attatctgta
      601 actacaggtg atatcaacga cgatggttgg atggatgtgt tcgtaggaaa cgactacatc
      661 atgcatgact atacttacct gaaccaaggt aacggaacct ttaaagaatc tagccgcgat
      721 gtgttgatga aaacagcata ttatggtatg ggtaccgatc tggccgacta taataatgac
      781 atgctcctcg atatgttcac tgttgatatg gacattgaag gaaattacgg cacgaaaaca
      841 tttatgcaat ctaataagca aacattcctc cgcacattgg tgaacggtgg ttatttgcaa
      901 caatatggtc gcaatgcatt gcaattaaat aacggtgtcg gtaaattctc cgaggttgca
      961 aactttgcag gtgtatctac taccggctgg agctggagcc cattgttcgc cgattatgat
     1021 aatgatggtt ataaagacct ttatgtatca aacggcttcc tgaaagatag ccatatggat
     1081 atcatggaag tttacgtgaa attgactcgc gcaaaccgct tatccgattc tacagaatat
     1141 tatgagttgc gtaaacaaat acctgaaagc tcagtactcg aatggcctaa cgctatgttc
     1201 cataacaatg gtgatttaac gttcactgat gttcgtgaag actggggatt gtatttccca
     1261 tctacttctt atggcgctgc ctatgccgat tttgataatg atggcgacgt agatatcgta
     1321 gttagtaatg caaatatgga agcatttatt gcccgcaata acagcagtaa attaaatacc
     1381 aacaaatatt tacgtgtcga cctcaaaggc gaaaaaggaa atcctgaagg ccttggagca
     1441 aaagtgtatg taacaacttc tacaggcaca caaatggtgc aaatggaaac ggtgaaaggt
     1501 tacctttcat gttcagaacc ggttgcccat ttcggaattg gctcagctga aaaagttgat
     1561 caattagttg tacgttggct agatggaaaa gaaaatgtgc tcacaaatgt tgctgcaaat
     1621 caaacagttg ttgttgatca cagcactgca atgaaaccta taaaatctgc tgttccgaaa
     1681 ccggatgcat tatttgtaaa tgcaactgaa gatctgaaag ttgatttcgt acataaagaa
     1741 aatgaattcg atgatttcga tcgcgaattc ctcattccta aagaactcag caatctcggt
     1801 cccggtgttg ctgttggaga catcaatggt gacggcatgg acgatttctt tgttggaggt
     1861 gctaaaaatc aaagcggtgc catctacaca caaactgcaa atggcacatt tgcgaaaact
     1921 tcctttgctg aaggaactga agatatccaa agtgaagaca tgggtgccat tttcttcgat
     1981 gctgatggtg ataatgataa cgacctgtat gttgtaagtg gcggttcaga tttccctaaa
     2041 gatgatgtgc atttgcaaga tcgtttgtat atcaatgatg gaaatggaaa attctcatgg
     2101 gctaaagatg cattgcctaa aatgatctcc agcggttcag ttgtagctgc tcaggatttc
     2161 gataaagatg gcgacctcga cttgtttgtt ggaacacgca tcattccggg taattatcct
     2221 tatcctacca agagctatat tctccaaaat aacaaaggta aattcacaga cattacgtcg
     2281 tctgttgcac ctgatctcga aaaaatcgga cttgtatcag cagctatttg gactgatgta
     2341 aataatgacg gtgattatga cctcatgctt accggtgaat ggatgccgat tactattttc
     2401 gaaaacaatg gcggtaaatt taaaaatatc actaacgcag caggcctttc agaatctacc
     2461 ggctggtgga atagtttaac tgccggcgat ttcgataacg atggcgatat ggactatgtt
     2521 gccggaaatt ttggtactaa cctgaaatat cgtccgaaag aaaaaccaat cgaattgttc
     2581 tacgacgatt acgataataa cggcacacac gatatcatca tgggttactg gcagcacgac
     2641 aaactatatc cggaaaaaac acgtgaacgt atgattgaac agatcaagga tgttgaaact
     2701 attttccctg actgggatag ctacggacgt gctgaagttt gggatttcta tggtaaaaaa
     2761 cgcatggaga attctcaatt gcactacaaa gcaaattcat tctataccag ctatatagaa
     2821 aaccaaggca acaacaaatt tgcagtgaaa cgcttaccaa atgatatcca gatatcatcg
     2881 acattcggta tcgtgccaat ggatgtaaat caggatggca atctggatat cgttgcgcat
     2941 ggtaacttct atgaaactga aattgaaaca aatactcagg atgccggtat cggtaatgta
     3001 ttgttaggaa atggcgatgg cacatttaca ccattacacg cacgttacag cggattctat
     3061 tctgccatga atgcaaaatc gcttgccatg ataaaagttg gtgcagctaa aactccggtt
     3121 ttacttacaa ccaattcaaa caacaaaatg gaagccttca aactggcaaa aaataccgca
     3181 gtaggtgtga atgccaacga tgcatatgct gttatcactt tcagtgatgg caaacaacgc
     3241 aaacaggaat tctacaatgg agcaggttac ctcagccaaa gtgcgcgcgc cgttgttgta
     3301 aatgatgctg tatcgcaagt tgtggtatat gataataaag gcactgctcg cacaattttt
     3361 ggccagcaag tttcaatgaa taaaaaataa acccctaatc aaccgctcaa tacaacaatg
     3421 atgaagattc acaatgtatc ggttctgatt gcagcatttg cgatgttcac agtggcatcg
     3481 tgcaagctgt tttcaggtgg cgatgtcgat acgcggggta ccgcgatcat cgacccaaaa
     3541 cgcgaggtga aactcgacgg taataacgga aaattgtttg atatccttac agacaaatca
     3601 accggcatta ctttcgtaaa cgacctcact gaaaattaca gtgtaaactg gtggcgatat
     3661 tcatatattt ataatggcgg tgcagtttgt atcggcgatg taaataatga tgacctgccg
     3721 gatctctttt ttacaggtaa tcttgttcca agtcgcttgt ttatcaataa aggcaacttg
     3781 aaatttgaag attatactgc aaaagcaggt atcaccagca ataaagataa atggccttat
     3841 ggtgcatcta tggtcgactt cgatggcgac aatgacctcg acatttacgt gtgtatgagt
     3901 cgctgggatc aacctgaaag acgcgcaaac gaaatgtggg tgaataatgg cgatggcact
     3961 tttaccgaaa aagcaaaaga ccttggcctt accgataact cctattcaac acacgccaat
     4021 ttcttcgatt atgacaacga cggcgatttg gatatttatc tggtaacaca tccggttgac
     4081 tttatcgaca aaaacaaaac caaatacttt cagaaaattg agcaaggtaa aaacctgagt
     4141 aaccattttt atatcaataa tggtgacggt acatataccg aatctcattt gaaagtcggt
     4201 atcaataacc atggatatgg attgagtgct tccgttggtg atgtgaatga cgatggatat
     4261 caggacgtat tcgtttctaa cgactacgcc atgtatgact atctctacat aaacaacggc
     4321 gatggtactt tccgcgatga gtcgctcact gctatcaaaa agacttccat caactcaatg
     4381 ggaactgata tcagcgactt taataatgat ggtttcctcg atatgtttgt tgttgacatg
     4441 gacatggaag ataactatac ctataaaact ttcatgcttt caagtcaagt ggaagtgatg
     4501 cgcattctta tcaatgccgg ttatggttat caaaaccgct ctaactcatt acagttgaat
     4561 aacggcgatg gcactttctc ggaaatatct cgtactgcaa atgtttctac taccgactgg
     4621 agttggacaa cattctttac agatttcgat aacgatggct ggaaagatct tttcatagct
     4681 aacggatttc ttcgcgattt ccatgtggat gaatcagaaa cttatcacaa attacgccgt
     4741 gcagtgcgca tcgaagatag tacagtttat tacgaagtga gaaaggaact tcctaattat
     4801 gtactcgatc accctaacta catctatcga aataatggtg atcttacctt cgaagataaa
     4861 cgcgatgatt ggggtatcta ctacccttcc attacttacg gtggaggtta tgccgatctt
     4921 gaccgcgatg gtgatatcga tatgattaca agtaacgtga atcaatcacc ttgggtatat
     4981 cgcaataaca gcgagaaaat gaataccggc aataactaca tcaacttcaa agttggtgga
     5041 tatgcaaaaa atactgccgg tttaggtact aaagtgcgca ttttcaccgg cgataatatg
     5101 cagtacattc aatacactgc aagtcgcggt tatatcacta ccattatcga caacatccac
     5161 ttcggcgttg gcaaagcaac aaaaatcgat cgcgtggaag ttgaatggtt ggatggtacg
     5221 aaattgatgc ttactgatgt tccggttaat caggttgtaa aacttgatca tgcacaggca
     5281 tctaaatcag tgcaattccc tgtgcgtgtg aaaggcgata aaatttttgc taataacacc
     5341 gaatcattag gtacagattt cgaacataag gaagctgatt acgacgactt ccttcgcgaa
     5401 ttcatgttgc ctcataaaat gagcacactc ggttccggaa tagctgttgg tgatgttaat
     5461 ggcgatggca ctgacgattt ctatatcggt ggtgcagccg gtcaatcagg tgcattatat
     5521 acgcaaaacg aaaatggaaa attcacccgc tcaagctgga ctcaaggcat gaatgacggt
     5581 ctagcatctg aagatgccgg tgccttattc tttgatgccg atggcgatgg cgataatgac
     5641 ttgtatgtag cttcaggtag caacgaattt gcttccaacg ataaacgcta tgcagataga
     5701 ttctatcgca atgatggcaa gggcaatttc acttacgata gtagtgtact gcctgccatt
     5761 aatagtagca gttcttctgt ttcaggttgc gactttgata aagatggtga tatcgatcta
     5821 tttattggtg gtcgccaggt tcccggaaga taccttgcac ctgcttcaag ctatatcctt
     5881 aaaaatgatg gtggtaaatt ctctgacgct accgcgcagg tggcaccaat gttgaaggat
     5941 attggtatgg taactagtgg tttgtggaca gacttcaaca acgataataa tatcgacctc
     6001 attcttacag gcgattggat gtctattacc ttcctgaaaa attctggcgg taaattcacc
     6061 gatgttaccg cttcaacagg attgaacaat gctacaggat ggtggcaaag tattaccggt
     6121 gccgatctgg ataatgacgg tgatatggat tatgtggtag gcaatttcgg aactaaccga
     6181 cgctataaaa acaccgtttc ttcacaagga aaaccattgc cactggaagc attcttatac
     6241 gatttcgata agagcggtca ggaagattat atcatgtgct attatcagca tgataaactt
     6301 ttccctgtta aaacgcgtga gcgtatgatt gaacagattc cttcatttga agagaaattc
     6361 ccaacatgga atgaatatgg tatcgctacc gttcaagata tgtttggcaa agatctggat
     6421 aatgccattc atcgcaccgc ttacatcttc cattcttcag ttttaatgaa tgatggaaat
     6481 ggtaagttca cgatgaaatt cctgccaaac gaagcacaga tctctaccat gtttggtact
     6541 attatcgatg atttcaatga agatggcata caggatatcc tgatgcatgg taacttctac
     6601 aatactgaaa tcgagattac acgtcacgat gccggaaccg gcctgttgtt gatcggaaag
     6661 ggtgatggca cttttacacc taatcgctca tatatcaatg gattcaagag cgatggcgat
     6721 gcgaaagcat tagctaccct actcgattct aaaaccaaac aaccggtaat tcttgcagga
     6781 aacagccaag gtaaaatgct tgcattcagc cttgtaaaac caacaaaaac tatcgagctg
     6841 gcagcagcag atgcttatgc aattgtgact cgcaccgacg gcaaaaccac tcgcgttgaa
     6901 ttatacagtg gaagcggcta tcttagtcaa agcgctaaat caatccgcat aacaccactc
     6961 gtaaaacaag ttgatgttta tgattataaa ggcaatatgc gcaccgttta tcccggtggc
     7021 aaccttgcct cgaaataatt tatatgaatc aaataaaaag ccggtaatta gttaattacc
     7081 ggcttttttt atgtctatat tttagtcaat atcaaattac aaaaaatcag gtggattcac
     7141 catgcggtat tgcccattca acttcgctct ttcacgcata tctgacacct gttttacact
     7201 tggcaaaatg cgtttgatat gctctacgat gagcttttgc cgctcagttt ctcgcacgtg
     7261 tcgcagcaat tcgtactcat ctttaagatc gaaaccaacg taatgcccaa gtcgaaatga
     7321 tttaaatgca aaatcaggtt tctccatctg ctcgcgaaca tctaaaagcg agaataattc
     7381 tacagtcagc tcttctaatt ccaatgccaa cctgctgtgc ttgtcttcta cattcggcaa
     7441 taaggagatg attgcaccgg catataattt gtcgggaatt tcgcgcacta cctccaacaa
     7501 ccgaaacact tgcaatcccc gcacctgaat atccatttca tcattcggat ataccttgtg
     7561 gatcttgatt aattccattt cagtgccatg gtcaagcatt tgattgttgt caaccacagg
     7621 aatgccaaaa gttttgtttt ccttactgca ttctgatatc aattgcttat aacgaggttc
     7681 aaatatgtgc agattgagct gctcgcccgg aaaggcaaca aggtttagtg gaaatatggg
     7741 aaggaatttt tgcatcgtat ttcttatttc ctctcaccgg ctcttacata aaccggtaaa
     7801 ctatttactc gtggcggtat aggacaggaa taattatgac tataaaaaca ataaggttga
     7861 taggctaaat taaaatctaa ttgcattgcg ccgggttgaa gcttatgcac atcaagatac
     7921 cttccccctt cataagtttc ctgtccattt gtcttatcat aaaacggaac aaaataagat
     7981 tccttatgtt caacctgata gtaggtgaca tttcgatata cttccagcat gcaaatagtg
     8041 tccttcagtt taaattggat attgccggca atctgataga tatcagccac atcgccggta
     8101 gcttgcatgg taaatatggg accattggcg ataggcgaga aattccctga taccctatag
     8161 cttgaatcaa tggggtaaaa ttgcaaaccc ttgaaattag ctctggcgct gtcgctcaac
     8221 ggactccctt cgcctgcaaa atatgcctgc tgatcatttc tttgcttttg aatgacctgc
     8281 gcataactca aatgaggatc gggtaatgtt gtttgctttt gcctgcagga agatgcaaac
     8341 aatatcaaca cgcaccccat taacgagaag taaaatctgc tgcccatcgt aggtcaaatg
     8401 tagaaaaaat ctttacttta tgcatccatt taaaccgcca tggcatcgct gttcgatatt
     8461 gaagctttcc gcgactactg tttgtcgaaa aaaggtgtta ccgaagaatt tccttttggt
     8521 ccagatacac ttgtctacaa agtaatgggt aaaatgttcg cattgtcggg gctcgatgaa
     8581 ataggttttt cgatgaactt gaaatataat acagaacata ttgatgaact tcgcgataaa
     8641 taccctgctg ttcaacccgg ctatcatatg cataagcgac attggaatac agtaatggtt
     8701 gacgggagca ttcctgttaa agaattaaaa tcgttgatcg atcattccta ttcgcttgtt
     8761 gttgatagcc ttccgaaaaa attgcaggca gaactgaatg ccttgaagta aatgataagt
     8821 gaagaccaaa tactatatat cgacaatcat ctgattgcca tcaataaaaa gccgggagag
     8881 atctcgcaat ctgatgttac aggcgatgta agtttgctgg aggatgtaaa gctgtttatc
     8941 aaagaaaaat ataacaagcc cgggaatgtt tacgtcggac tagttcatcg cattgaccga
     9001 ccggtaagtg gggttttgct gtttgcacgc acgagtaaag ctgcagagcg actttcgcag
     9061 atgatcagag acagggcatt taaaaaaact tattgggcgg tagtgaagca aatgcccaag
     9121 gagcctgaag gccgattgga aaattatttg ttaaaaaatg cacagatgaa taaatcgttt
     9181 gtggcaaaaa atgaaaaagg tggtgccaaa aaagcagtgc tgcactatcg attaatttac
     9241 tcacttgaaa gataccatct acttgaagta attttagaaa caggtcgtca tcatcaaatt
     9301 cgcgcacaat tatcggcaat tggctgtccg ataaagggcg atttgaaata tggatttgac
     9361 aggagtaatc cggatggttc catacatctg catgcgcgtt caataagttt tgagcatcct
     9421 gttacacatg ctgcaatcac catcatagct cccccaccgc aggatgtcat ctggaatgcc
     9481 tgcttgaaag ttgcggaaca acaataaaaa aacccgccaa aagcgggttc aaatatttta
     9541 aagaagttta ttaattattc atcagaacca ccgcccaatc gagcatcagg gaacataaag
     9601 ctgattccga ggtggaaacc tacaaatgca ttgtgcgaag caagatatct gttctcgcct
     9661 tgtgcaggac gtaactcacg agtttccttc gcattgatat caaacaatcc gaaataaccg
     9721 cggaacgatg gcgtgaaata gagcttatca ttcacataaa tgtctgcacc tattccaatc
     9781 aaagcgccaa catcaagttt tgtaaaaaat tctgtcccgt ctgaacccgc atttccgcta
     9841 aaatcaggaa cagcatcaaa tggagcatga aatcctgcct gctctaccgg tttttgatca
     9901 tcaggatcca aatcatataa atcagccgta tactgctggt ctgcaccaag taaatagcca
     9961 aattgcaaac cacctaacat ctgatatttg atcgcatcct tggaatcacc ctccatgtat
    10021 ttaaacaata caggcacttg caagtaagtc aaatcaacat atctgtaagt aagcactttt
    10081 gtgcgcaaat caggctttgt tgaatcggta ataggaccga agtctttaat gatatcggaa
    10141 taatcttgcc ccatttgact gtaattcagt tctacctgcc agccgaaatt atcatcccag
    10201 ttatatccca gcgacatacc gccgagcttg ccatacttat actcatagtc catttgtgag
    10261 tattcgtaat tattctgatt aattatccag atgctgttca tctgtgcatg gaaaccaaaa
    10321 tgcagtccct gctgtgcgga ggctacacct gctattccca ggaaggcaat gatgagtagt
    10381 gtcgcttttt tcataccaat tggttggatc gttcgcagcg aatatacaac ctttgtttat
    10441 ttcactatga aggcaatcaa caatttagcc tttttatgta tataaaacag ttcgtggagg
    10501 gtagtactca tttgtcacat atttccatct tttataattc ctatattact gtatatcaat
    10561 attttaaata tttttttgtg gatatatttt attgtttacc atgtcactga atgtcaaacc
    10621 tagaaacaaa atttaccccg tatcttcgct ccatgttcaa cgtgcatatg gccggtcagt
    10681 ttttgcgata tcaagtgcgc gcattgagtc gctacgagtt gcattcgcca tttctgttcg
    10741 atttggaaga gaaagtactg cacgacaagc gccatttcta cgcctttgat gagattgaat
    10801 atcgcaggga gttactgctt gctgaagatc aaaccatttc tgtgagcgat tttggcgccg
    10861 gcagccatgt gcataaatcc ccggaacgac gggtttccga tattgccaaa acaagcctga
    10921 tcaccccaca tttcggacaa atgctctacc ggttagtaca gtatttacaa ccggttaccc
    10981 tccttgaaat gggcacatcg ctaggaatta gctccttgta tatggctaaa gctgtccctg
    11041 gtgcacaact catcacgctt gaaggcagca gtgcgattgc acaaatagcc cgaaaacaac
    11101 atatagcact ggatgcaaag aatattacaa taatggaagg tgaattcaat gctaccctac
    11161 cctcagcact gaatgcctta aaaaaaatag acctggcatt tatcgacggc aaccaccgat
    11221 ttgaacctac ggtcgcctat tttgagtcta tttatccata tttacacgaa cactctatat
    11281 tggtgtttga cgacatacac tggagtaccg gaatggataa ggcatggcat gtcatcaaat
    11341 ccgatgcgcg ggtaacttgc agcatcgatc ttttctacaa aggaataatt gcctttcgcc
    11401 cggaattccg tcagccgcag catattgttt tgcgctatta agaccaatta acacagttta
    11461 accgcactgt aaggcaatct gccatccttc ttaattacct ttatgcctta taataatacc
    11521 tatatgagaa aacgaatgat gataagcatg tgtttactcc tgctggcagg aatcatgcat
    11581 gcgcaatctc cactaccctc caacttcttc ctcaaaacct tgcccaatgg cttgcaaatg
    11641 ctggtgattg aagaccctac cgttccactt gctacgattg aaattgttgt aaagaatggt
    11701 tcttatactg aggacagcac attcaacggc ctttcgcaca tgtatgaaca catgtttttc
    11761 aaggcgaata aatcgctgcc aagtcaggaa gctttcctcg aaaaagtgca agaactgggt
    11821 gtagtattta atggcaccac cagtaatgaa cgcgtaaatt atttcatcac attaagcaat
    11881 gcgaaattag gcgaaggtct tgtatttatg aataatgcca ttcgctaccc attatttctg
    11941 gagcaggaaa tgaaaaatga aaatcctgtt gttgacggcg aatttcaacg caacgaatct
    12001 aaccctgtat tcttcctgtt cgacgatttg aatcgtcata tgtggaaatc aaattactat
    12061 cgcaaaaatg ctatcggttt acatgatgtg attcttacct gtacacctga aaaaatgcgt
    12121 atcatccagg agaaatatta ttatcctgac aatagtctta ttgccattgc cggcgatgta
    12181 aaacatgatg atgtatttgc acaagtagaa aaaatctatg gcgattggaa accgggtggc
    12241 ttcgacccat tccagaaatg gcctatacct gaattcgaac ctttgaaagg tgttgagaat
    12301 tttgttacta ttaacgaaaa tgctcagatc ccgatgttta tcacaggcta tcatggcccg
    12361 gatactcgaa atgatgtaaa agctacttat gctgccgatg tttttagtta cttattaaat
    12421 ctgtcggaca gcaaactgcg ccaggagcta gtggaaacag gtcttgctta tgatgtcaat
    12481 gttggttatc aaacatgtaa atacaccgga cctatcaata ttttccttgt accaaatcct
    12541 gcaaaagtga gcgaagcatg gaaaacatta caggaaaata tcgggcaatg ggctagtccg
    12601 aattacttca ccgatgaaca attggaaaca gctaaaaatc agctagccat tcaggatgct
    12661 tatgacaaag aaagcatcag caattatgta catactgtta cctattggtg ggcatctgca
    12721 tcgctggact acaatacaaa ttacattaaa aatgttcagg cagtgactcg tgaagatatt
    12781 cagcgttatg ttgaaactta tatcatcgac aagccgcatg taaacggatt attgttacat
    12841 ccggatatgc ttcagatggt tgatgtgaaa gcacttggat ttgaagtaaa acaataataa
    12901 catttgaacc tcgaaaaata cgttatgaaa aaaatacaaa taaccatcgt tttattggtg
    12961 acactgcttt cagcaaatcg cttgacagca caggatacca aagaatatat ggtggatggc
    13021 atcaaggtca ttcataaaca ggtacctaaa gatgtaatca gcgtgcagct atttattgaa
    13081 ggtggcactg ccaattacac gaaagacaaa caaggtattg agaatctcac attgaatttg
    13141 atgatggatg gcggcacaac cggcatgtcg aaactcgaat tcaaatcagc cgcagaaaaa
    13201 atcggcacta cgtttaatgc cggcagcaat atagacgcag gcacacttag catgaattgc
    13261 attaaactat tttgggacga ttcatggaaa ctattttccg acgctatact gcatcctgca
    13321 tttggcgaag ctgaatacaa tttgctccgc gaacaattaa ttgcaggtgc acaacaaagc
    13381 atggcagatc ctgacacgta tctagcctta ttatcccgcc aggtagcatt tgaaaataag
    13441 gattatgcta aagcaccgga aggaactccg gaaacgctaa ccggcatcac actcgatgat
    13501 gttaaaacct attacaagaa aacaataggc aaaaaacgcg tattccttgt agtagttgga
    13561 aatatttctc aagaagacct gactgccaaa attcacaata cgcttgcagc attacccggc
    13621 ggaagcccgg catcatctga aagtcgcaca ttgatcacgc aacctaaaac atatattgaa
    13681 gatcgcgaca tcgcaaccaa ttacattcgt ggtatcatga gtgctccatt tgcgaacgat
    13741 gcggaaggta ttgcgtatcg cgtagcgatt tcaattgttg gtgacagata tttcaccgaa
    13801 ttgcgcacaa aacgcagtct cagctatgct ccggcagctt tctatgcaaa cgcaggtatc
    13861 accaatcctt atggcgttat ttatatcact accatcgatc ctaaacaaag tatgcaggtg
    13921 atggtggatg aattaaataa aatcaaaaaa gaaggtttta ctgaaaaaga attgcgcgat
    13981 aaacaacagg aattcttaac tacctattac ctcacactcg aaacaactgc taatcaaagc
    14041 aatgcattgg gtatggcgga aatgagcggt ggatgggaaa atcttgataa aataaccgct
    14101 gctgtaaaca gcctgaaact tgaagacatt aatgctgttt tcgacaaata cagcaatgcc
    14161 attgtttgga catatttggg taaaaaatct gctatttctg aagctgattt ccctcaaaca
    14221 acaccaaagg aaaagaaaaa caaaccgtat tgattgcggt attcatggta taaaaaaacc
    14281 tccgtcaatt gcggaggttt ttttattgct gtaatttatc gtcactcgtt caaaaaaatc
    14341 tgtttgagct cttttgcttc ttccggtttc atacgtccgg ctaaaatcaa acgcaattct
    14401 cttcttcgca acgccgaaac aaagcggcgc ttctcatcgt cattctcagg ttctatttcc
    14461 ggaacggaaa ccggtctgcc gtcatcatcc aacgcaacaa aagtgagata agcctcattg
    14521 cattttactt tttcatgcgt gcggaaattt tctttgaaaa cttctacgaa tatctccata
    14581 ctcgaattaa atgctctcgt catacgcgcc gaaatagtta cgatgtcccc tattttgatc
    14641 ggacgaccga aactcacatt atctaccgca gcggtaactg ctactctatt ggcatgcttg
    14701 ctggcacata taccacaggc aatgtccatc catttcaata gatttcctcc cattaagttg
    14761 tgcataggat tggtatcgtt cggcataacg atctccgtaa aggtggttgc cgattctgaa
    14821 acacgcttcg aagccatagt gtaaatattg ccgcaaaggt aaccattatt gccctatcgc
    14881 cttcagcatg ccgatttgtg gcattttgag tatcattgta tgacttatca agctgccgta
    14941 actgaactat ttattttatt tatatgtata tacatacaaa tgcgtaattt agccctatgg
    15001 agcgcaggaa attcattaaa gcaggaacat ttagcttatt agccatgcaa ctaaaggaat
    15061 tcagcaatac ctttcaacag gaacaagata tcgtaatgcc tgcaatgttt gttgggcatg
    15121 gaaatccgat gaacgccatt gaagacaatg aattcagtcg cggatgggag gcccagggaa
    15181 agttattacc tgctcccaaa gcaattttat gtatttcagc ccactggctc acgcgaggaa
    15241 caagagtagc tgccatggaa aaaccggaaa caattcatga tttcggcgga ttcccgcaaa
    15301 ccttatttga tgtgcaatac cctgctcccg gatcaccgga atttgcaaaa gaaaccagca
    15361 ctgtcatcac caaaacaaaa gtagaacttg attttgagtg gggccttgat catggatgct
    15421 ggagtgtatt aaataaaatg ttcccaaatg ccgatatccc ggtatatcaa ttgagtatcg
    15481 actattatca aacacctgct tatcactatg cattggctga agaattgaaa tcactccgca
    15541 aaaaaggggt attgataatg ggcagtggca atattgtgca taatcttggc atggtgcaat
    15601 gggcagatac tgcgtatgat tgggcgattg aatttgatac gaagatcaaa gaattcattg
    15661 atgcgcgaaa tcaccaaggt gtaatcaatt ataaagattt gggtaaatcg gcattactgt
    15721 cagtgccaac caatgatcac tttttaccat tgatatatac actcggcgtt accgatacca
    15781 aagaagcgat ctcatacttc aatgaaaaaa ataccatggg cgccgtttcc atgcgctcat
    15841 ttgtggcagg attatcgtaa tatccttttc aggtattcaa caatggtaat ttagataaaa
    15901 cctgcagcta tcattaatgc cttttctttt taaataaagg ctgcttgtta gtattgcgtt
    15961 ctcgggaagt aattttctcg cgcaaacccg ggtttgattt ccttccggca agtaattctt
    16021 cctgcactac aatattcgaa gtgcccacat ttggatttgg attaacagtg gtagcagcta
    16081 ttccttgccc accatgtgga acaggttctg tatgttgaac aaatccgaca gacaatgaag
    16141 tgaaggccgc cggagaaatg cgtgccgact gtccgaacac aggcatggcc gcaataacaa
    16201 aaaggataat gatgcaacgc ttcataacag taaaacgaag gtgtattgca aaattatatc
    16261 ggaaatgcat cggtgcttca attttgattg aatttgacaa tgctgtgaaa tatgcttatc
    16321 acagaattag cggttaatag ttgtgatgta ctgattacca acacattagc ctattttaat
    16381 cgaaatgaag ccaagcgcat tgtgtatatt tgccctcaaa atgcaaaata catgaacctg
    16441 cacgaatatc agggtaaaga gctcgtaaaa cgctttggcg ttaagattca ggaaggcatt
    16501 ccggtagata ctccggagca agcagttgaa gccgcacgtc agttacaggc agctacaggt
    16561 gctaatattt gggtggtaaa ggcgcaaatc catgccggcg gtcgaggtaa ggccggaggt
    16621 gtgaaagtgg caaaaagcct tgatgaagtg tatgaaaagg cttcaaaaat attaggtgct
    16681 acactcatta ccccacaaac aggtgcaaac gggaaaaaag tccataaagt gcttgttgcg
    16741 gctgatgtat actatcccgg cgcctctgat accaaagaat tttacatgag catcctgctc
    16801 gatcgcgaaa aaagccgcaa tgtaattatc tattctacgg aaggtggtat ggacatagag
    16861 gaagtggctg aacatcatcc ggacaaactc cataaggaat gggtagaccc tactgtaggt
    16921 atccaggcat ttcaggctag aaaaattgct ttcaacctcg gtttgagcgg tgctgccttc
    16981 aaggatatgg tgaaattcgt gacgagtctt tataatgcat acgtgggtaa cgactgttca
    17041 ctgctcgaga taaacccggt tttgaaaacc tctgatgacc agatcattgc ggttgatacg
    17101 aaatgtgttg tagatggcaa cgcgctgtat cgccatgcag atattatggg catgcgcgat
    17161 atcactgaag aggatccgac ggaagtagaa gccggtgaag caggtctcaa cttcgttaaa
    17221 ttagatggca acgtgggatg tatggtgaat ggagccggat tggcgatggc aaccatggat
    17281 atcattaaag catcaggcgg taatcccgct aactttttgg atgtgggtgg aacggcagat
    17341 gcaaaacgcg ttgaaacggc atttaagatc atattgaaag atccgaatgt aaaagcaatt
    17401 cttgtaaata ttttcggtgg cattgtgcgt tgcgatcgcg ttgcacaagg tgttatcgat
    17461 gcatataaaa acatcggcaa tattccggtg ccaattatcg tgcgcttaca aggtactaat
    17521 gccgaagaag ctaagaaaat gatcgacgac agtggacttc aggtgcgttc tgcaattctt
    17581 ctgaaagaag ctgcagcact ggttaaggaa gctgtgctat aatgtgaatt ctaacttttt
    17641 aaaatagaaa aggatggcaa ccccatcctt ttttttatat ccctaaaata aaaatacccc
    17701 accaattttt caaccggagg ggtaagttgt tcaagtatga aaagaaattg cttgcgattt
    17761 gaactcagct caaaaataga tttgaattag tattttcaag tattcgcact ttagccgggg
    17821 cacttgaagc agcccccgcc tggattcttg cggcgggcag gcttaccgaa ggttgtaaag
    17881 gccattcaag taacgaagaa taaagggcga agacatagaa aagacaccaa aggtattatt
    17941 gagatgtgtt ctacataatc attctaatta cgcaatctgt aaacgccgaa ttaattcagt
    18001 gtttgcacat gtttagcttc atcaagtatg cgcatggcac tttgtaaaat agcagtatct
    18061 tccaatcgca ctacaccgcc actgattccc ttttcaaaat ggacgtcggc aatttttccc
    18121 ggagcttcaa aatgttcagc tccgaaataa tttctgacgg catccacttc aatgtgcagt
    18181 tcctcggcga tgcgctccat actgccgtgt ggcaaggcat ctttgatgtg tcgcaactct
    18241 tcgaatgtga taaccataat tgttcgattt aggtgagaaa attgtattgc aatataccac
    18301 gatactagcc cgctttttcg atatttccaa gaaaaaagtg aggttttttt cgtttaattt
    18361 catcatttct ggcattgctt ttgtggcgaa taaaaaaacg gggtttctat acatattttt
    18421 gtatgaattt catacgaaac aggtaatttt catgcgttat tctatgcatc ttctcatgtg
    18481 aaccctaaac cctccactac catgcgggca aaactcatct ggcctgggct tcttgcacta
    18541 ggtttgtacg cttgtaacgc cgacaggaat acaatagcca tcaaaaccgg cgatttccct
    18601 gaaacaatag aactcaggca gaatctggaa atacatctca accagcatgt atttaacgat
    18661 tcgctattcg acaaatgggt tgatgccgat ttgttgcaga tagatccacc tgtagaaggg
    18721 cgatttaaaa tcgtttccag cgatgctttc tatttctcgc cttctaaagg atttgcagaa
    18781 agtgaagcct atacagcaac acttggtaag gatcttttca attataccga tctgaaatta
    18841 gcactgaaat cagcaccggt aaactttcat actactttgc tcgacctgac ggcagtgaat
    18901 acatattggt ttctggaacc cacttcaggg gaagtaaaat tgaatgccgg actgcaattt
    18961 acagcctcag tggatccgca aacacttggc gatatgttaa cagtgaaagt tgatggcact
    19021 aataaaactt caagtattac ctccactcaa tcagctgata atatggcagt taccatcagt
    19081 ggcatatctc caaaagctaa agatcaagac ctgaacatag acattcgcgc cggaattgga
    19141 tcaccgggtt cacgttacaa aacgccgaaa cctttgcata tgaaagttcc tgttgctccg
    19201 gtggaagatc ttgagatcag agatattact gcaaaccatg acggcatagt gggaactata
    19261 acggtagaat caaatcaaca gttcagcaat actaatcttg aagaactggt gaaaattgaa
    19321 ccctccattc cagttaccat tgaaaaaact ttgaacggac tagtcgtaac cagcgagcaa
    19381 tttaatgcct cacaaaccta tgtgattata ctttcagata aattgagagg tgtagccgga
    19441 ggaaaaatgg tagataccta tcagcaatct gtcaccttcg gagaactgga gccatccatt
    19501 gaattcacac aacaaaaagc acgctatctc ggcggtaagg gagaacgtaa tatttcagta
    19561 agaattgtca atgttcctaa agtgcaggtc aatgttgtga aaatttatga aaacaatatc
    19621 cttgcattct tgcgcgacgg cttctcatgg gattggtatg aatcatatga tgcagaagaa
    19681 gaatattggg actaccacga ttatcaaaat gtaaatacat cgttatatgg caacgttgtt
    19741 tatacggaag aaatagaaac ttcgaaactc gaaaagtata attccgccag agtattgcat
    19801 ctcgatttta cagataaatt ggaacgcttc gatggtatat atgtagtaac cgttagcgct
    19861 gctgaaaaag tatggctaac cgatgctcaa atcgtttcaa tttcagacat cggcctcata
    19921 gcaaaagcaa cggaggatga actggtagta tttgccaatt ccattcagac cgctgaaccg
    19981 atgagtggta ttaaagtatc atttatcagt tccaacaatc agaaactgta tacggcaagc
    20041 accgataaaa acggagttgc aaggtttacg ggattgaaac aaaacatgcc cgatttcaat
    20101 gtaaatctaa tcacggcaaa aaatggaagc gactataatt accttccctt tagcgaaact
    20161 gaagtaagca cttcgagatt tgatgtaggt ggtaaaagat taaatagttc tgatatagat
    20221 gctttcctgt atcccgagcg cgatttgtat cgtcccggcg aaaccatgca tattgcaggc
    20281 attttgcgta catacgactg gcaattgctt gaccgtatcc ccgtgaagat caaagtaatc
    20341 tcgccgagcg gaaaggattt caaaacactc cgaaaaacta tcgatgatca aggtggattt
    20401 gaaacaggaa tcgatatccc gaaaaatgcc gttacgggaa catataccgt tcaattgttt
    20461 tcatcgaatg atattttatt ggcatcggaa tatatcagca ttgaagaatt tatgcctgat
    20521 agaatcaagg tttccggttc actcaataaa cagaattact atcccggtga aagtgttcag
    20581 tcgaccatag ttgccaataa tctatacggc actcccgcag ctgagcgcaa ttacgaaagt
    20641 gaatttaatt tgcaacagca ttttttctct tcaaaaaaat atcctgctta taattttcag
    20701 atttccggag gtaccaccta tttcccttcg caaaatgccg aaggccagac agatgaaaat
    20761 ggtcaagctg ttcaatccta caccatagat caaacctaca gcaaggttgg tcttctcgac
    20821 ggtcggattt taaatactgt ttttgacgaa tcgggccgac ctgtttacca agtgcagaat
    20881 ttcaccgtca tcacacaaag caatctatta ggtataggcg aatttgatta ctatgtaaaa
    20941 gtaaaatcgc cattgcgtat tccgatgatt gctgtcgaca aagacgataa aggatataaa
    21001 actcaggacg cccatgtaga aatcattaaa tacgattatg aaacggtgat cgagaaacat
    21061 cgcaattatt attcatatcg ctcaaacaga aaagagaaga tacttcaatc ctatgataag
    21121 aaaatcagtg gcgatcagga tggcatttgg ttcaccccgc aacaatccgg agaatatcaa
    21181 atacgcttat atttaaaaga tgctaaagca tacgttgaaa agtcattcta tgcttatgga
    21241 tggggttcaa cacaaaacaa ttcttttgaa gtaaatacag aaggtaacat caatatcatt
    21301 tccgataaac caaagtatga tcttggcgaa gaagctgaat tcttattcac taccccattc
    21361 aatggaaaac tattggttac agttgaacgc gaaaaggtaa ttgaatatca ttacctggac
    21421 actgaaaaga aagctgcttc attaaaaata aaaatgaatc agtcgcacgt gccgaatgta
    21481 tttgtaacag ccactttatt ccgaccaaac gacgaaagtg gtatgccttt gacagttgct
    21541 catggcatca tcaatgtaga agtaaaaaat cctgcgaagg cgataccgtt gacagtgacg
    21601 catgaagatc attcacgcgc aaatacgaaa caaactatca atataaagac ggcaccaaat
    21661 gcgcaggtaa ctgttgctgt tgttgatgaa ggcattttgc aaatcaagaa tttcaccaca
    21721 ccggatcctt acaactggtt ttacatgcaa catgctttag gtgtgttcag ttatgatctc
    21781 tatgcttatt tgtatcctga aatttatctt aagaagatgt tgagcggtgg cgatgccatg
    21841 atgaatgaag gcagattgaa tcccatgacc gcaaacagag taaagcttgt gagttattgg
    21901 agtggtatcc taaaggcaga tgcatccgga aaaatagcat acaccattga tattcctcaa
    21961 ttctccggag atctgcgtgt aatggttgca gcatatcgca acgatcagtt tggtgccagt
    22021 gaatcgcata tgaaagtgag cgatccattg gtaatgacta cttccctacc ccgcttcctc
    22081 actcccggag atacgataga tgtgccaatt acacttgcca atacaacaga taaaaaagca
    22141 agcggtaaag tgaccatgtc tattgaagga ccgatcaaag tggttggcag cagtacacaa
    22201 caattcaatg caaatgcgaa caaagaagct cgaagtgtat ttaaaattgt tgcctgggga
    22261 gctacaggaa atgcgaaggt gaaagtccaa tcttcagcat taggagaaac atttaccgac
    22321 attactgaaa ttccggtaag accgcctgca ggattgcaga aatacagtgg ggaaggttac
    22381 cttgacggca ataaatcgat aacggttaat atgcaaccaa accttatcag ttcatctgcc
    22441 aaggctcgcc tgatgatcag ttataatcct cttgttcaat ttgccgatga tcttgattat
    22501 ctggtgcaat atccgcatgg ttgcgttgag caaatgactt caagtgcgtt cccgcaactg
    22561 tattatgccg atatggtgaa atcattctac agtaaggagc gtccggatct tgaccctgca
    22621 tataatgttc aggaaactat acgcattttg caaagcatgc aaatgtataa tggcgcgttg
    22681 acatactggc ccggaggtgg atatgaaagt tggtggggaa gtatttatgc cgcacatttc
    22741 ttgcttgaag cgaaaaaagc cggttacgat gtagaccaaa aaacactaga taaactatta
    22801 ggttatttac gcacgatgtt gaagcagaaa aaaacctatg tgtattatta tgataaaggt
    22861 ctcagtgttg aaatggctgc acaggaagta ttttattcta tgtatgttct ggcggtatcg
    22921 ggaaaggctg aaatgtcgat gatgaattat tacaaagcga atctcagcat ggtaccacag
    22981 gatggtaaat atcttttggc catgagttat atgctggcag gtgataaacg cagtgcagat
    23041 aaattgttgc cgggtggctt taccgatgca gatcccaaac aacaattcaa tggcagcttc
    23101 aacagtccga ttcgcgacag ggcaattgcc ttgaactgta tgttggaggc agatcctaat
    23161 aatcagcaaa taggacgctt gtcacaacaa ctatcagaga tggtaaaaaa tgctcgctat
    23221 atgaatacgc aggaacgcgc atttaccttc cttgcattcg gaaaattagc gaaacagtcg
    23281 aaagaacata atgcgaaagt tgtaattaaa atcggaggca aagaagcagg cactttcgat
    23341 ggcacggatt tagttattga agataccaaa atggcttctt caccggtaac acttaattcc
    23401 tcaggtagtg gtcgcgttta ttatttctgg tatgaggaag gtgtttctgc tgacggctcc
    23461 gtaaaacttg aagataattt tttgagggtt cgcaagtcat tttttaatcg atggggtcaa
    23521 ccaatagatc ttaaaaatat ccatcagaat gatttggttg ttgttaagat cagcttggta
    23581 agtgctaccg gcacctacgt tgaaaatatc gcgattacgg atatacttcc tgcaggattt
    23641 gaaattgaaa acgaccgact ccgtgccact aattctatgc catggattca aaatcagtcg
    23701 gaagcacaac atgaagatat tcgcgatgac aggatattat tgtacacgga tgctacggct
    23761 aaggaaaaat atttctatta tacagtcaga gcagttacta ccggaacatt taaaatggga
    23821 ccgataatgg ctgatgccat gtataatgag gaatatcatt cttacaatgg cggaggatgg
    23881 gtgaccatta aaaaataaga aatgaaaaag gggtgttaaa gccccttttt ttatacctta
    23941 taaattcaat ttgtttttat tgtttgtggt gtgggttttt tcgtactaag acgggaaggg
    24001 gcgcaaggcg cgaagcatgt ggtgtggatt ggtggtgtcg gttttttcgc gcggagggcg
    24061 cgcttgagtt attgatgcga tgaagtttat cgactattaa aaaatctgcg ggaaaaaatt
    24121 ttatgaactt tttttacagt gcgttagtta accggcgtca ggcatttttt aatacttaac
    24181 tcgaatacta ctattaaact cctccggaac agaaattaca gatccttttt cgttgtagaa
    24241 tgtcatttta tactctcctc tatccaaggc ttcatttaca tctgtatatt tatttagaaa
    24301 atcctctact accagcactt ctttcccttt atcatccgtt aaaatcactt ttacatttgt
    24361 attcgggcgt agaacatgct gcaattttgc cattttattg gcaccattta ctacccatgc
    24421 aggtgagttt ggatctatag ggtgcttacc ttttccggat actactgtgt cttttactac
    24481 ggtaacttgt aatgcatcat ccaccacaac ttttcctgaa taattatttg ttccgtcaga
    24541 aatgagatat ttataatttc cggctcctgc tatatctaat tccagcgttg gcccaacctg
    24601 agcggcatcg ttattgaaaa caacggctga atcttttaaa accgtcaact tatatgtcgc
    24661 tgcaatttta tcatatggga tgttgagaat ggttttgcta ttgataataa acgccatagt
    24721 ttgcatatcg gcagcagtct catcacctat tgtatcaacc gtaacctctc cgggattttc
    24781 caaaaaccat tgttgcgaaa cggggtcagt tcgccagaaa tcaaccagat ctttcgatac
    24841 tttttcaatt cccttttcag taggatggac tccatcattt gaaatcatat ctctggttaa
    24901 ggtatagcca tctttgcgtg gtttctcacc ttgcgtccag aaatacggtc cccaaagtat
    24961 taacggagaa acggcatttg cacctttgaa agctaattcc tcgctgccat tgatctgatt
    25021 ttctattaat tgcttacaca cccatccatt gatataggct ttcggttcgc gatgtttatc
    25081 ctttgcatcg gcaggcataa aatcagttgt atgtcggccg gatacataaa acaattttac
    25141 attcggataa tgctccttgc aaaacctaac catgtaggtg aactcctttg tcagttgggc
    25201 aatgcgtaaa tcgaaattat tctcgcgatt gcggagatta tcctcttgaa accaaataac
    25261 ctgaatctga ttaagatcca ttcctgcttc cctaacgcgc tggtctataa cactccaata
    25321 gtttgctgca ggattcatga tatcactcaa atcctgtccg ccaatcccac aatttacaaa
    25381 cgttagctcc ttattaactc ctgctgcttc cggaatctga tgttccaagc ccttgctaaa
    25441 catggcaacg gtacttgcac caagggcaat aacccctatc ctacccttag gatccggttt
    25501 cccggacttg tcgagtggtt gaaccgactg agccatagcc attgcatcgg catagaatgc
    25561 agcaggcatg gaattgctgc cattgggata taatccaccc tggcgaccca taaaagtgcc
    25621 tgtgccgagt tcatcaattg gaggcaaatc attcacatcg ccgctttgtg ccttaacaaa
    25681 aagggaacta aaaactgaaa atatcgataa tagcatcaaa cgcttcattt gaggggtttt
    25741 tattgaattt taacaaatat cataccttta ttgaaaagtt aggcgttttt tttgctgcaa
    25801 ggttatgatg tctatttcta ttctcacacg ttatatattg ttgttactga gtttcctccc
    25861 cttttccgcc ttttcccagg ctatgttggg ttatactgat atgcaaggtg attattatgt
    25921 aagcgacagt ggaaggatag tgcatttgga acatcttcgc gtaaaatctt cacaaccggc
    25981 tgcaaacagc atcgcttata tttcaaacac gggaaatctg gtatattaca gtaatcatga
    26041 acaggtaaag ctggatatca ccaaccccac tttttataaa aataccgatc actatttgta
    26101 ttacagcaca ggaggaagtt tttccgtgta caatggtaaa gaacgcaagt acctgggata
    26161 catacaacaa tatccgtatg ccttcgccga tagcattgca gctgtgcacg actattccgg
    26221 atatttctat acctatgtaa atgacttgtt catcgaattg gaaaatcaac cggttttaaa
    26281 ggtaattgca ggtgacaata ttctcgcata cgtaaatcat attggtcagt ttaaagctta
    26341 ttatcaaggt aataaggttg atcttgatga ttacgctccc atcactgcac aggctgccgg
    26401 aagtatcatc gcttacatcg ataattacca atacatgaaa gtatattatc aaggtagcat
    26461 tcatgaatta tttaatttac cggaaataaa ttgtcttagc actacaagtt caggggaccc
    26521 tcttcttgct tcatggtgta atggtccggt agtcacagac gttcaaagcg aactacctgt
    26581 tttccaagtg ggtgatgaca tggttgccta tatcgatgat atggggcgat ttaatgtatt
    26641 ctacaaaggc aatattgttg cattggaaag tcagccgcca aaacaatata ccgtggtcga
    26701 cagagtttta tattatacag acgaaaatga ctatctgaaa gtattttgca atggtgaatt
    26761 atcgattgta gaaacctata ccccatcgcg tattgaagct gatggtgaag tattggctta
    26821 tatggatctg gacaaacgat tgaaggcatt ttatttaaat acatctgttg atgtttccga
    26881 aaatatagta ttagatttca gtttgaataa caccttactg atgtatagtg agatcccgaa
    26941 taaatacaaa ttctattccc tcgaataaaa atgggattac tccctacatt tagaaacttc
    27001 ttttcacgtg cacgtatttt gcggataatt catcgtgcgc ttgccacagt gctgatagtt
    27061 ttcaccttgt ttctgctgtt gaacctggca tttccgatgc atgttaagat tgattattcg
    27121 caaactgtaa ctgcagcaga tggaacagta ctgcacgcat ttctgaccac tgacgataaa
    27181 tggcgcatga tgaccgagtt gtcggagatc acgccggaaa tgcaaaaagc aataattttt
    27241 aaggaggata aatatttcga attacatccc ggagtaaatc ctcttgcgat atgcagagcg
    27301 ctgttcaata atattcgcac cgggcaccga acatcagggg ccagtaccat caccatgcaa
    27361 gtagctcgta tgctggaacc caaggatcgc acttacggaa ataaagttgt tgagatgttc
    27421 cgcgccttgc aattggacat gctgtattct aaagaagaga tattgcaaat gtatttgaat
    27481 ctggttcctt atggcggtaa tattgaaggc gtaaaagctg catccatact ttatctgcaa
    27541 aaaatgccgg cagtattatc actttcagaa ttaacggcat tgagcattat tcctaatcgg
    27601 ccaacctcac tggtacccgg tcaaaacaat gcacagatca ttgttgagcg aaataaatgg
    27661 ttggaaaaat tcagaaatgc agatttgttt tcagatgaca tcatagatga cgcgcttgca
    27721 gaaccgttta acgcaaagcg aactgaaatc ccgcaactgg caagacattt cagcacacgt
    27781 atgcggatgg aatttcctgc cgatccaatt attaaaacta cactccaacc acaactgcaa
    27841 tcgcaatgtg aggaagtcat gagtaattat gttggtcaat tatgggcgct agatatcaat
    27901 aatggggctg caatagtcat tgataataaa agtcacaaag tggtggctta tgttggttct
    27961 gcaaatttct ttaatgagcg tgatggcggt caagtagacg gtgtgcaagc agtccggtct
    28021 ccgggaagta cgttaaagcc gctattatat gcgatggctt ttgataaagg gatatacaca
    28081 cctcaatcta ttgtaagtga tgtcccgatt aattacaccg gttaccgacc ggaaaattat
    28141 gatgaagact atcatggaaa tgtttctata acttatgcac tcgcaaattc tttgaatgtt
    28201 ccggctgtaa aaaccttgca tgaaattggg atggaagatt ttctgaacat gttacagcgc
    28261 gcaggatttt catcactcac caatcaaaga aaagatcttg gattatctgt agcattagga
    28321 ggttgtggta taacgctgga ggaattgaca cgtgcctatt ccgcatttcc aaataacggg
    28381 aaatcgccgc atatcaggta tttacaatcc gacaatgata caaccgcaac gcctgtattt
    28441 acggaagccg ccgcattcat gactacagaa attctgagcg aattaacccg acctgatctt
    28501 ccattatttt accagaatgc taagaaagtt cctcgtgttg cctggaaaac cgggacatcc
    28561 tacggtcgtc gggatgcctg gagtatcggc tataatgaac aatatacggt tggcgtatgg
    28621 gttggcaatt tcagtggacg cggtgttcca gaattgagtg gtgcaaatgt ggctacacca
    28681 ttgttattta atatttttca ggttttaggg tcagatgttg atgcggactg gaatcgcatg
    28741 ccggaatctg ttcaattcag atatgtatgt gaagttagtg gtgaaattcc tactgcaaat
    28801 tgttcgcatg tagtaatgga ttatttcata cccggcattt caaaaaatga tagttgcaca
    28861 catttaatag atgtaatggt tgatccgaaa gaaactataa gctattgcaa tacttgtaaa
    28921 cctgaaacgg gttataaagt caaacaatat caaaattatt ctcctcagat catcagctgg
    28981 atgcaggaaa aacatttgcc atttgaaagt attcctccac acaacccgaa atgtgaacgc
    29041 atgttcagcg atcagatgcc ttcaattaca tctcctgctg atcaaatgga atatttgatt
    29101 gaaaaagatg atcctcagcg catcatgttg tcagccaatg ttgcttcaga tgtcaaaacc
    29161 atattctggt atatcaacga taaattactc acttcagtta atgcaggaga acctgtattt
    29221 ttcgatcccg ctgaaggaaa aacaaagatc agttgcagtg atgataaagg caggaatgtc
    29281 gatatcgaga tcatggtcga gttcttttga gggaaatcgt gagtgggtca gtgggtnnnn
    29341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnactc accactcacc actcaccact
    29461 cacgattcac gactcacgag tcacgattca cgattggtag caagtagtgt gcagcttgat
    29521 acatttaata atcaaaaagc cccactcaat agagcagggc ttttgtcttt ttattaaagt
    29581 gttatgattt aattattcat cccaccaaac aggtaaagtg atatcaccta caacttgcac
    29641 attgctattg tagagacgct catcaataca agttgggaag cgacgaggaa tttcatcata
    29701 gatcgcatcc ggatttggtg tcaatgcagg aatatttgtg cgacgccagt cggcccaaga
    29761 ttcaggctga gtgaacatag cgatatattt ctgcgtcatg atcttatcaa gtgtgattga
    29821 agcgccggtt tcacttgcgt gagctgcttc ataaacagga tcaggagcac ctaccgtggc
    29881 gagaatattc gctttcactg catcgtttaa tgcggcagcg gcagcatcat tattgccaag
    29941 acggaggtta gcttcagctt cgatgaattt catttcatag taagtgagca atggcaatgg
    30001 tgatgcatca gatgaataca attcaccagt atttgatgca gcaggatcca cactgtttgt
    30061 agctacacct gagtatccgc caccatcatc cgtagttgca tacaaaggca gacgtgggtc
    30121 gttggttgaa gataataagt cgataaagaa tgcacccatt ttgatgtaat tagcgcgagt
    30181 ggtattgaaa gagaaccaag ggttcaattc atttccattt gtaccaaaga ttgcattcgt
    30241 gttatctgca ttagaggtaa cacctgattc agcgatatgc atcaatgctt cattagcaga
    30301 acctgttgca tcgcgtttgc tcaagcgatt ggcataacga gccttcatca tatgagcagc
    30361 tttgatccac atgcttacat caccattatg gatgaaatca tccgaagcag gcaagaaagc
    30421 atttgaggtt gatggcattg ataaatcggc tatagctgca tctagttctg attgcatagc
    30481 agcaatgata tctgcttgtg catcatattc aggattcaaa gccgcatcgg taccatcgag
    30541 tccttgaagc gcttgagagt atggcacatc gccccagaaa tctgtagcga ttccgatagt
    30601 catacatttc aatacgcgtg caattccgcg ataatatgga ttttcatcac cggcgatatc
    30661 aatcaatgtt tgttgattga ttaaagcatc ggcgtataca gttttccatt cgttggtatt
    30721 acttaattca gtgattttat aatcacggat atcaatcatt tggaaatcgt taccggcgca
    30781 ctgctgtgtc agaatagaag cggtgcgggt aagttgcccg gtataaagtg tctgcattgc
    30841 aacctcagaa actgagagca acagatcggg cgttacttct gtcggagagt ttggcgaggt
    30901 acttacctca tcaacccagc cgctgcaacc ctgtgccata atgatggcag ggagagcaag
    30961 tatcattata atctttttca taattcagat tttaatggtt tagaaagcag cgcttactgt
    31021 gaataaatat gatttagttc cagggttatt aaaccagtcg aaacctgaga tgtttgaacc
    31081 tgcacctgta aggcttgttt caggatctac acctgggtaa tcggttttga ggaagaggtt
    31141 gcgacctgtt gcagccactg taaggccttt gatcacattc ttcttatcga ggttgaattc
    31201 gtaagcgagt tttacttcgc gcaaacgcac ccatccgcca tcataaacag cttgtgaaga
    31261 tgcgccgaaa tcgccctggt agctttgcca ataatcagca ggtgaaatag cgatgtcatt
    31321 ttgtgaatat gtgccatcag cattttcaat tacaccatcg attacataag tttcagtacg
    31381 atcgcctgaa gcttcgctga taccgatacg gttcatacga gcaatagttc cgcaccagat
    31441 gtcaccacct ttgcgaatat caagcaatcc acttacagtg aatcctttgt aagtaagtgc
    31501 agtgctgatg ttcatcaacc aatccggata tggatcgccg ataacaccac tcactgtatc
    31561 gaaaattggt aagcctgatg atggatcgat taataaatca ccatcttcat tatatgccca
    31621 ctgtgaaccg taaagtaaac cataaggctg accaacgatc acataagcac ccggatcacc
    31681 gaaacctgct tcaatctcaa tttcatcaac accatcagca agttgtgtta cttcgttttc
    31741 gtttttgctg aagttaccac caacattcca tgtcagattt ttcttcttaa tgatatctgc
    31801 attcaatgtc aattcaatac ccttatttgt catcgcacct gcattgtcat atacataaga
    31861 gaaacctgat gtagatgcaa tcggtttggt gagaaggata tcagatgatt cctggatata
    31921 ataaacggca tcgaggtcga tacgtccttt ccagaatttc aattcgagac caaattcagt
    31981 tcctgtcaaa cgctcagggc taagatcagg attacccaat gtattcaatt gtgagtaacc
    32041 gaaacctgat tgtccgaggt aagggaaact ataaccatct gtgaaaccat cggtatacag
    32101 tggtggtatg aagtaagtgt cagcactgta aggttgaggt tcgatacctg cttgtgcata
    32161 agcaaaacgc aatttaccga atgaccagat attgtttgta gtcaggatat cgctgaatac
    32221 aaatgcaacg ctggctgatg ggaagaatgc tgaactttga ttaggtccgt aagtagatga
    32281 aaattcatca cgacctgtca aagtcaagaa taataattcg cgataatcaa actcgagatc
    32341 gccaaagaag gctgcgttac gttgtttagt tgtatactgt gaagaataca agtcggcagc
    32401 attcgacaag ttataaaaat caggaatggt aaggtcgcga ccgcgtgaat attgattgtt
    32461 gatatcaata tcagtgaggt tattacctac agttaatgta attcctaagt cttcatttac
    32521 ttgatgtgta gctgttgcaa taaggtcgct gtatatctga cgatagcgaa tagtattttc
    32581 ttcgatttgt ccgcccggcg catttgcagg gtcgttagaa ccgatagaga aaatctgttt
    32641 acgcaaatca gtatagttat cagcaccaac gcgataagta acatttaacc aggtccaagg
    32701 agtgtaagtt gctgaaacat tgctcaacaa acgatttaca ttatcagtgt acgggttatt
    32761 atttacagtc cagtatggat tatcatatgc tgagaaatag ttgcggttgt cgcctgaagg
    32821 atattcataa ccggctccaa gatcgtagct tgttggtgtt ctaagtaatg ataacattac
    32881 accagaaagg ttactaccat tttgagcgcg agtaccacca ctggtgatat agttggcatc
    32941 aacggcaact ttcagtttat cataaattcg catgtctgaa ttaacgcgaa ctgaagttct
    33001 gtcgaattgt gtatttggaa caataccttt atcttgtgta ttaccaatag aaagacgcag
    33061 agatccgcga tcagttccac cggaaacact caagttatta ttataggtca taccattttg
    33121 gaagaattct ccggggttgt cggttggcgt gatgccaata tttgacatca gtggtcccca
    33181 tgaattggcg gtacctgcag aaccatcgtc atctgttccc caaaggaagt caggacccgg
    33241 atcagaagta atataattac cttcaggatc aacgtttcca tcagcatctg taaaacctcc
    33301 acctgaacct tgagcataca attgttgctg agcaggtatt ttatttacct ggctcacttc
    33361 aagtgaagat gagaaattaa tgtggactgc tttatcacct gctttattac cacgttttgt
    33421 tgtgataatg atagcacccg aacctgcgcg tgaaccataa agcgcagcgg ctgcaggtcc
    33481 ttttaggatg ttgatgcttt caacatcatc agggttgatg tcaatggcac ggtttgagtt
    33541 acttacacca ttcaatgttg ggttgaaagg atagtcgccc gcaacagtac tagtagtaga
    33601 gttgtcgatt ggcaccccgt caattacaat aagtggttga ttttcgttgg taaaagtact
    33661 cggaccgcga ataatgatct tagcagatgc accgggagtt cctgcagaac caaccacctg
    33721 gatacctgca gctttcgctg aaagtgcttc cacaaggttt tcctcaccgg aacttgcgag
    33781 gcggtcgcca cccacttcct gtgtagatac ccctaacgaa agtttttctt tcgggatacc
    33841 cagggcggtt acaaccaccg tttccagacc taacacgtct tcgtccatcg caatgttcac
    33901 cacattttta ctgccgatgg attcctcgag cgttttaaag ccaagaaatg tgaatactaa
    33961 aacatctttg tcttgcggaa caacaagcac gtacttacca tccaggtctg taactgtgcc
    34021 caccgttgtg ccttttacca tgacggttgc gcctggcaga ggctgattgt cggttgccga
    34081 cgttactgtt ccggtaatgg tttttccctg tccgaaggcc acagatacca taccaaacat
    34141 taacagcagc accgaggcag ctagttttct catagatttg gttttgaatg aaataatagg
    34201 tttaatcttt taactgaccc tgtaaagtct aatgttaaaa tactttaaac aacaaactta
    34261 aacgtctttc tgatttttta gcaattttca ggtaaaaaaa agccccgaaa gtgaatacac
    34321 cggggactta ttaacaaatt ttcctctttg ttacttgtgg gtatcgtagt aaccgaacac
    34381 tttttgaacg atttctgagg ctctggcggc cggatcctgg cgtattttaa gctcctcctg
    34441 ttctatcata tagaatagcc catccaacgc tttttgagtc acataatctg ataaatttgg
    34501 attcatgtcc tctaccaatg gtattttatt ataataatta acggcatcac tccaatattt
    34561 tgttgcacct acttcactga gcgatttgtc aataacgggt ttgaattttg ctgttaattg
    34621 tgctgttgtt gtttttttca aatactcagt ggcagcatcg tcggcaccca ttaaaatatt
    34681 catggcatcg ctaaatgtca tagatcgaat actgttaacc aaaatcggct tagcttcagc
    34741 agcagcatcc tcagcggcgt gatttacaga attgatcaca tcatcacaca ttttattcaa
    34801 tccaagatcg cgcagcgtgg tttctatttt ctgcgcttcc ggaggaaatg gaattttcac
    34861 agatggattt ttcaggtaac catccacttt tgccagtgta gctgaaccat tggaagcccc
    34921 ttgcaccaag gcttctatca atccattaga ggcttcctga tcggtaatcg tggtgctcat
    34981 aactgtattt aaaacatcct ccattacctg aggatcgcaa gtggcccctg taaataaata
    35041 catggtgatt gcggctaagc agaaatttct aaacatgaca attgcatttt gatgccaaat
    35101 taaatctttg tttgaaatgt tgggcatgca tcgcacaatt atcagtataa tttgttgtta
    35161 tggtcagtaa ttccctttga caacttatac ctttactaca aaatacaaat atgcgggctg
    35221 cactcatttc cattggcgac gaaattctta atggcacgac aataaatacc aatgcaagtt
    35281 ggatctcaac agtactgcaa ccgttgggtg tagatattca tgaggtgctg gctatttctg
    35341 atcatgcaga tcatatactc ggcacgctgc acgcctatgt tggcgaaatg gaaattatca
    35401 tcattacagg aggcctgggc ccaaccaaag atgacatcac gaaaaaaacg ctctgccgct
    35461 atttcgatag caccctcgaa ttccatcaag acatttacga caggttaaaa gctgccttcg
    35521 ccaagcgaaa cattccattt acggaaaaca atcgcgatca ggccatgtat ccgcacaact
    35581 gcgaggtgct gcataatgct ctaggaaccg ctcagggcat gcttttcaag cgaaatggca
    35641 gcgttattgt aagtttaccc ggggtccctt ttgagatgaa aggtctcatg accgaccagg
    35701 tattacctcg tttaagggaa gaattgcaat tccctaccct gcttaaccaa tattttatga
    35761 caagtggtat cagcgaaagt tttctggctc gtcaactcga gccactggag gctatgttgc
    35821 ccggtcatat tggattagcc tacctcccat cgcccggagt tgtaaagttg cgattgacag
    35881 caagaggctc cgatgctgca acactcaagg cagaattaga gatgttaagc aatgaaatgc
    35941 gacgaatact gggatccggt atttatgctg aggaacctat ctcgatagag gaacatttag
    36001 gaaaattgct tgtcgagggc aaggcaactc taggatgcgc tgaaagttgc acaggaggca
    36061 agattgcgca taagatcact gctattccgg ggagttccaa ttggtttaaa ggcggggtga
    36121 ttgcatacga taatcaattg aaaatcaata ttttgcaggt taatgaatcc atcatttccc
    36181 aatttggtgc cgtaagtgaa gaaacggtat ctgctatgct ggatggcgct ttaaccgttt
    36241 tggggaccga tttcgctatt gcaacgagtg gaattgccgg cccgacaggc ggaactgccg
    36301 ataaaccggt tggtaccgta tgggttggag tagccggagg aggcaaaaaa gtggtccaca
    36361 aatatttttt caacaagaat cgagagatca atattgaata tgcttgcatg tttgcgctgc
    36421 atgagatgcg cttactgttg actgaaatcc ttgttaaacc atcttaaagg gcttattttt
    36481 gcgaatactg ttaacatagg catggtgtgt ctgtgggtta cggctagtgt actatttaca
    36541 attcgaatta aacatggcgc agaaagaact gatcatgccc aagatgggcg agagtattat
    36601 ggaagcaacc attctgaagt ggttgaagaa agaaggcgat accgttagcg ttgatgaagc
    36661 ggttttggaa attgctacag acaaagtaga cagcgaggtt ccttcaccct ttgcaggtac
    36721 tttgaccaag atattattta aggaagggga tgttgttcag ataggtaagg cgattgcaat
    36781 acttgatggc gacggatcag ttacaacaaa aactgctgca ccggagccgg cagtaaagac
    36841 tgaagtagtt gtagaaacac ctaaggtagt tgcgccggta gtgcaaactg aagttcctgt
    36901 agttgccggt gacaggtttt atagtccact ggtattaaat attgcacgcg aggaaggcat
    36961 ttcaatggcc gaattagagc gtattccggg aacaggaaaa gataatcgcg ttactaaagg
    37021 cgatattctc aattacgtgc agaatagagg tgccggcaca acacaggcac cggtagaaca
    37081 acaagcacct gttacacaaa ctaaaactga agaaccggta attgtaaaaa ctgcttcagt
    37141 aagtggtaat gtagagatcc gcgaaatgga ccgtatgcgc aaacttattg ccgatcatat
    37201 ggttatgagt aaacgcgttt cgccacacgt tacatcgtat gttgaagtag atatgacgcc
    37261 aattgttgaa tggcgtaatc gtatcaaagg aaaatttgaa caacgcgaag gacagaaaat
    37321 aacctatacg ccaatattta ttgaggcaat tgcaaaagca attaaagatt ttccgggtat
    37381 taatatttct gttgacggca ataatattat ttacaaaaaa gacatcaata tcggaatggc
    37441 agctgcattg ccaagtggta atcttatcgt tccggtaatt aaaaatgccg atcgcatgaa
    37501 tatggttgga ttaaccatgg tcgtgaacga tcttgcaaac agagcgcgtg ccaataaatt
    37561 aaagccggaa gaaattcagg atggcacatt cacgcttacc aacgttgggt catttggcag
    37621 cctgatggga acacccatca tcaatcaacc acaagtagct attatggcag ttggtgctat
    37681 caaaaaacgt cctgtagtgt tggaaaccac acaaggtgat gtaattgcca tcagacatat
    37741 gatgtttatg agtatgagtt acgatcaccg agttgttgac ggtatgttgg gcggatcatt
    37801 cctgaaacgc gtagctgatt atctggaagg atttgatact gaacgcgaat tataatttat
    37861 tggtttttca tatcaataaa ccacacacat gtacatagaa caactatata cgaattgttt
    37921 agctgaagcg gcctattaca ttgaaagtaa tggcgaggca gctatcattg atccacttcg
    37981 cgatacggct ccatatattg aaaaagctgc ttctcgcggg gcaaaaatca aatatgtttt
    38041 tgaaacacat tttcacgcag attttgtttc aggccatatt gatctagctc gtcaaaccgg
    38101 agctcaaatt gtttacggac cagaggcaaa tacatcctat gatgtagtaa atgctgcaga
    38161 tggtgaaata tttcatgtag gctcacttca aattcgcgca ttacatacgc cgggtcacac
    38221 attggaatct acttgttatt tgttgataga tgccaatgga aaagaccact gcatttttac
    38281 cggtgatact ttatttgttg gcgatgttgg tcgtccggat cttttggatg gtattatgac
    38341 caaagaagaa ctggcatcca aaatgtatga ctcgctgaat aataaaataa agccgttagc
    38401 agatgatgtt gtagtatatc ctgctcatgg ccctggaagt gcctgtggta aaagcatggg
    38461 gaaagaaaca ttttccacca ttggcattca gaagcaaact aattacgcac tgcagccaat
    38521 gacgcgtgat gaatttatcg ccgccgttac cgatggttta tcgcaaccac caaaatattt
    38581 cttcgaagat gcacgcatta acaagcaggg ctatgaagat gtagaaatgg taatggcaaa
    38641 aaatgcaaaa ccactttcaa taacagcatt tgaaaccgcg gtggaatcag gtgctttaat
    38701 attagatacc cgaattgccg atatttttga aaaagcattt attcctaatg cattaaatat
    38761 cggcctcaac ggaatgtttg ctgtttgggt aggcactttg gttgatatcg atcaaccgct
    38821 tgttttggtt actgaaccag gaaaagaatc ggagtcagtg ttgcgacttg cacgcgttgg
    38881 atatgaaaat gtattaggtt ttcttgaagg tggaattgat gcctggatac aatcaggcag
    38941 aactattgaa acggtaacca ccatcgaccc tggcagcatt aaaccatata tgggtaatga
    39001 cgattatatt gtgctggatg tgcgcaaacc atctgaattc gaaaacgaac atatcagtgg
    39061 cgccattaat atacaattaa gtgagattgg tgctcgttta catgaattgc aacctaacaa
    39121 acaatatatt atgcattgcg cggcaggtta cagaagtatg atggcagcat caattttaaa
    39181 agcgaatggt ttaaacaatt ttgtaaatgt gcgaaacgga tggacaggaa tcaaaaaaac
    39241 tgatttacca actgagtcat ccgtgcctga actaaattaa tgttgctgcg aaaagcaaaa
    39301 tgcgaaaact gatattgatc atagctgtta ttatttcgtt cacctcctgc gataatttca
    39361 aggagagtga agctattgaa catatcacac gcacatggac ggtttacaaa tatgaagttg
    39421 acggatacga tcaaacgcca ttatttgtag ccacatatgg cacctacact ttagtttgtg
    39481 agaaagatca ggattatact gaaacatata ccgtaggtgc aacacctgtt actattaccg
    39541 gtatttacat tttcagcgat ggcgataata ttctgacatt gtcagatgaa tttcaatcgc
    39601 gtgtgtttaa tgttttaacg cttacgaata atgaaatgac tttagaagat cgcaatgcag
    39661 atactaatca ggtttattac cttgaagcag tgatctgaca aaatacaatt aacaaaaaag
    39721 gcgatgatca ctcatcgcct tttttgttgt tatttaccct ggcgttttac atcgccttta
    39781 tcttcaaatg atttgaaaac tgcagcaggg tcatattttt taactgcctt tcctttgcgt
    39841 atgattttga tatgattgat cttatcgcct tgttgaatag cgttcaccac atcctgacct
    39901 tcaaccacat gtccgaaaac agcatgttta ttgtcgagcc atggcgtttc tttatgcgta
    39961 ataaagaact gacttccgtt ggtgaatttg ccggcatttg ccattgataa aattcccgga
    40021 cctgtatgac gaaggtcgct atggaattca tctttgaaag cataacccgg tccgccggag
    40081 ccattgcctg aaggatcacc accctgtatc atgaaatcag caatgacacg atggaaggtt
    40141 agtccatcgt agtaaggtgt gcccagtggt ttagcggtat tttcaatttt tccttctgcc
    40201 agtcccacaa agttggcaac tgtgagaggc gctttctgga attccagaaa tagcaggata
    40261 ttgccttttg ttgtttgcac ttcggcatac aatccatcgg gaagatgctt gtatttttta
    40321 cgatcacttt gaccgaatac tgccgaatgc atcagcacgg ctagtaataa caatacactt
    40381 gtttttttca tatcacgatt attgatttat tgatttgtat ttgcttcggg catggcataa
    40441 aattttggca acacctcttc aaaggttttt atggtatcct caagcgactg ctttgatgag
    40501 ccgccggaag cattcatatg tccaccacca ttaaagaaat ttcgtgccaa aatctcaaca
    40561 ttcacatcgc ctttagacct cagcgacatt ttaacttttc ctttgtcttc ggtaaaaagc
    40621 accgctatat ctatatcctt catcgccaaa ggaagattaa caattccctc cttatcacct
    40681 atgctcaatt tatattgttc agcttctgcc atggttatac tcatgtatgc tgctctatat
    40741 gcaggtatga tctgcaattt ttgcgataag agatatccga taaagcgtat tctgttttct
    40801 ccatattgat tatagaggtc gttatgaata cgatcaactt taatgccata ttccatcaac
    40861 ttccctgcaa tagcatggac gtgtgcggtt gtgttgctat actgaaatat accggtatcg
    40921 agtgcaattc ctgcataaat gcacgcagcc atatcctgat ccaacagatg gatatcacct
    40981 gccatctcca taaatgtata taccaattca gcagttgaac tggcatggtc gttccacaat
    41041 ctgtaatccg tgaaattatc cggctccctg tgatggtcta tcatcacgct tacatatggt
    41101 cttgtcagtt tcattacatc ttcccccaaa gcttcaatac gtttgatggc attaaagtcg
    41161 agaaagaata tagcatctga ctgtgttaga atagcaaagc aggtcttgcg attgcgctga
    41221 tggatgtata cttgtttact acccggcatc caatcgagga agtcgggaaa atcgtcaggt
    41281 gcgattacat tggcggtatg acctttcttt tcgaggtaca tcttcatggc aagtgccgag
    41341 cctattgcat cgccatcggg acgcacatga gtaagcaatg taatccttct tggttgcttg
    41401 agccagttaa agagattggt cagttccata gttaattcgg gtactaaggt caccattttt
    41461 tattttttat taaaatggac gttacgaata cgtatctttg cgcccgaaaa taatatgtta
    41521 tggcaggaaa tatcacatta accatgatca aacccgatgc tgttaaaaag aactacatcg
    41581 gtcccatctt aaaaatgatc aacgaagcgg gcttccgcat tgttgcaatg aaatacacta
    41641 agctgagcaa agagaaagcc ggtgaattct atgctgttca taaagaacgt ccgttctatg
    41701 gtgaactggt agatttcatg agctccggtc cgatcgttgc tgcaattctt gaaaaagaaa
    41761 atgcagttga agatttccgc aaactgattg gtgcaactga tccttcaaaa gctgatgaag
    41821 gcaccattcg taaattatat gctgccagca ttggtgaaaa tgcagtgcat ggttccgaca
    41881 gtgatgagaa tgcagctatc gaaggcgatt ttcattttag caaattggaa cgcttctgaa
    41941 aataaatcac gaaaagaaaa gaagatccgg ccatgttgcc ggattttttt ttgctgcaac
    42001 gaattcaaat tttaccgtgc aataaatact tcactttttg attgtatgat ttattgatga
    42061 tgttttacaa tcaaaaaaaa acccgttacg gattgtgtcc ggaacgggtt aaccaaaaca
    42121 ggtttccaat ggcaaaaatg ttatctggaa acctctatct ttcgtatcgt agtttctgtt
    42181 gaagaactta actgaagcaa atataatccg tttgaaagtc ccctcaaatc aaatctgaaa
    42241 atattttttc ccacagtaca taactcgctg gttgtcagca ctttacgtcc ttctaaatcc
    42301 agaagatcga aggtaatgtc tgatgctgac ggcatgtcaa tttcaacggt catttcgtca
    42361 gtcgcagggt taggggaaac ggtcatttca gccatcgcca gtgcagttgc cgaaaccccg
    42421 aaattcaccg taaccacgta ggttttaggc gtataatcta ccggtgaaaa gcaggtataa
    42481 ttgaccggag aagtgaagtt ttgtgctacc cctcctaccg gatcaatata ctgtccggtg
    42541 tagtctatag tgggcactaa ggctgttaca tcagttcctg ccggtacggt cagggtgata
    42601 gtacctgctg cctgatcgat agctccacta gctgccggag tggtgaaatt gaaaaggacg
    42661 atactgtttg gataggtagt gtggtatcca tcgatatcat ttttatcgcg aggaaggagt
    42721 ttccagttac taaaac
//
LOCUS       DAOQOU010000006        42658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_361_length_42658_cov_5.550243, whole genome shotgun sequence.
ACCESSION   DAOQOU010000006 DAOQOU010000000
VERSION     DAOQOU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 42658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42658
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_42658_cov_5.550243"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLM90_01000"
     CDS             complement(<1..280)
                     /locus_tag="PLM90_01000"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ87969.1"
                     /translation="MENVTVTAFIILLTAISSILAFNNSVMRGSALFYPHAMHQRGEW
                     YRFITSGFIHADYIHLIVNMFVLWSFGSILEERLLPLVFQGYGRIVYVG"
     gene            complement(284..745)
                     /locus_tag="PLM90_01005"
     CDS             complement(284..745)
                     /locus_tag="PLM90_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HMZ87970.1"
                     /translation="MQIIFISVGKTSEKWVSDACSTYVKRLGKYVRFQWHETTDIKSL
                     PPEEMKLREFAAIKKYIEQGDHVVLLDERGAAYSSVGFSEFITRKQLENRKRLVFVLG
                     GAHGFHEDAYSMANETLRLSDMTFPHQMVRIIFLEQLYRAFTIINREPYHH"
     gene            complement(866..1441)
                     /locus_tag="PLM90_01010"
     CDS             complement(866..1441)
                     /locus_tag="PLM90_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87971.1"
                     /translation="MQIKIGALLAISILMLHACKESADNEDTAAIENVGEKGGDNSLQ
                     ELIDKEATTPDVVKQASGDQVLYNERFDYTITIPGFYTIKEKSNNGDGYFISSGDKDV
                     DIRVYGENIADNQVMAEMELNTCESKTSFQFSNGYPGILCMQSDDRYYYYDTPKTRVT
                     VYVHAPTRWFDRNNKLLDIMVKSLVVGKGGF"
     gene            complement(1446..3062)
                     /locus_tag="PLM90_01015"
     CDS             complement(1446..3062)
                     /locus_tag="PLM90_01015"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HMZ87972.1"
                     /translation="METIIPIGYWIFFIFTIVGTLAGLYKMFEKAGEAGWKALVPFLN
                     AWICVKITGKSRSWFIMLMIPVLNVVVWLLMSNELAKVFGKESFWAYVGSMMIPYIYF
                     LKLGFDEDVRYIGPHKSERKSVGREWADAIAFAVVAATLIRTFFFEAYTIPTTSMEGT
                     LKAGDFLFVSKFHYGARFPITPLAFPFAHHTMPVIGGKAYSDAIKMPYMRFPGLENIH
                     RGSSVVFNFPAGDTVYLPEQQNSYYSLLMNEATNLQINDAKLGNRPKPFSFYYNRVSA
                     AYRSSGELAYRPVDKRDNYIKRCVGLPGDSLWVSNGILYVNGEKAYVAPEQQKSYDVT
                     FKAGLGVDAAAGILLKMKLNSMDFEDTVNVTPNKIMITANMSIADSKLLAKNKQVESV
                     ILNHFDRMVAGYIGLFPNNPNQPLNSVDDFSPIYIPKKGATVKIDANSYYNYKRIIEA
                     YEHNTVTLDKQGRPYIDGKLLTEYTFKQNYYWMMGDNRHRSYDSRYWGYVPEDHIVGK
                     PVLLWFSWDKTLPLSERIANIRWDRMMHFK"
     gene            complement(3157..4629)
                     /locus_tag="PLM90_01020"
     CDS             complement(3157..4629)
                     /locus_tag="PLM90_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87973.1"
                     /translation="MSHSKGNIEGIDGKKGRDIVTLLQEPQVWIRAGVLFCLAFGVMT
                     MNLPFYGDQVTLVSAPASYIYEHGIHSPILPDDLNTGHFPLFPLLTAGFWVVFGRNLI
                     SVHLLMILCQLFMFSQVISFARVKLQKATLAVFTLLLLCYPVWWAQTAGMSADIMLCG
                     LFFMGLRAGISKKWVVWMIILGLLPLVSLRGWIWAFAMCIYVWSGEQFRFRWFMMIAL
                     RTLIALTPIIAYYTWQHAVSRWWIVPIDERYGEHRQLVDGKLLAGKGFEFLLRCIEFG
                     MVIPVVVMIMRGSALLNRFSITSNIGLIAISVFSIALFTLPFRGPILIRYMMPLHVLI
                     LIASAPFFVKAGGQLFRGGLVAMTACVFIAQHFIAYPQMRTSIFEYDWSGGSLSHLSY
                     FRFRAEGETYLKAHHIDDSEVGTYFPEYKSFALTDLVEDDTEYNAISPDSIPTSELLI
                     YSNVMNSVTMPMYDSIKAHYTLDKRWFSYPVTYEIYRRIP"
     gene            4672..6129
                     /gene="miaB"
                     /locus_tag="PLM90_01025"
     CDS             4672..6129
                     /gene="miaB"
                     /locus_tag="PLM90_01025"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HMZ87974.1"
                     /translation="MYDNNPTLEGLDQHKLLDETRQGEALGNEAMAEAATGKRFYIES
                     YGCQMNFSDSEIVASILHQHGFGLTNDYNDADLILLNTCAIRDNAEQRVRNRLKELGA
                     VKKRKPEALIGMLGCMAERLKADLLEEAKLVDIVCGPDAYRDLPMLVNTADDGAKAVN
                     VLLSREETYSDISPLRLDANGVCAFISIMRGCDNMCSFCVVPFTRGRERSRNAFSILA
                     EARDLFERGYKEVTLLGQNVDSYKWENPETGKVVNFAELMAMVASVDSTLRVRFSTSH
                     PKDITDEVLHTMARFDNICNYIHLPVQSGNSRVLELMNRTYDREWYLDRINAIRNIVP
                     DCGISTDVITGFCSETEEEHQDTLSLMEMVQYDLAYMFFYSERPGTLAARKYTDDIPH
                     ETKIRRLNEIIALQTKHSHARNQADIGKTFTVLVEKPSKRSALDMCGRNDQNKMVVFP
                     ATDVNPGDYVQVRVTSATSATLIGEMVGRGMHKAV"
     gene            6135..7382
                     /locus_tag="PLM90_01030"
     CDS             6135..7382
                     /locus_tag="PLM90_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ87975.1"
                     /translation="MADEIQSIKQRFGIIGNAPGLNYALSIAARVAPTNLTVLITGES
                     GVGKEIFSQIIHQYSTRKHGPFIAVNCGAIPEGTIDSELFGHVKGSFTNAVDDRKGYF
                     ETVNGGTIFLDEVGELPHGTQARLLRVLEAGEFIRVGSSKVQKTDVRVVAATNVDLHK
                     GIAEGKFREDLFYRLNTVPISVPALRERKDDIHLLFRKFTSDFSEKYRTLAITLDSDA
                     VELLRNYNWPGNIRELKNIAEQVSVLATDKMVTAAELHQFIPQQRNSILPMISGKQTG
                     NDFTERDLLYKVLFDMKQDLNDLKQFVLHMAEANHLKTDMSISAEMHDQPTFNELSTR
                     QPQLQTEFITKQPVVINPANSAAHAHVHEEVEESLSLIDKEKELINKALSKHRGKRKD
                     AAMDLGISERTLYRKIKEYNLDE"
     gene            7375..7887
                     /locus_tag="PLM90_01035"
     CDS             7375..7887
                     /locus_tag="PLM90_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HMZ87976.1"
                     /translation="MNKRRITYSLVCVAFLLSIAATCKVSFNGAQIPVDVKTLTINYF
                     PNQATLVSPSLSQTFTESLKDKFLRESSLTLTEQGGDWELSGAIVKYESTPIAPTGQE
                     TTALNRLSITVKVDFTSRKSEDADWSQNFMRYEDYESAKTLSQVEGDLLPKLSEQLAD
                     DIYQKLTQNW"
     gene            7887..8990
                     /locus_tag="PLM90_01040"
     CDS             7887..8990
                     /locus_tag="PLM90_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87977.1"
                     /translation="MFTEEFNSIITNPHSLQDVPLADLKVLAERYPWCQPVQVLYARK
                     LFLVESPQFEHQLHRTAILSYDREVLFKYINAEIPEVISVAPEPEVQPDQPVIVEEHQ
                     EIQPVVEISEPELDQISVEEFDPATEELIQEMIESIPEVHQEDEIAPIEMMSEETMEL
                     QEEEIEAESMLQEEMDLENLDHAVINDDMEAVASNPLPDERIEPDEAFIFEMPAYDIE
                     TELGTLDEADKFTLPNIPPEVAAEKNTEVFTDSFTGWLNRLGGSPTGKVVEQKASNAP
                     VKVYLRKQPTPGSDPAGSAHERLMNEQYAAELARKSIQSDDGLISETYARILVMQGKY
                     QRASEMYRKLSLLKPQKSDYFAALIDQINKKIK"
     gene            8992..9345
                     /gene="secG"
                     /locus_tag="PLM90_01045"
     CDS             8992..9345
                     /gene="secG"
                     /locus_tag="PLM90_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMZ87978.1"
                     /translation="MLFTVIIIIAIIAAVLLGLVVLIQNPKGGGLSGTFGGAANQIFG
                     YKRTTDDIEKITWILVAVVFVLCLSASALKPVETVTQQGGGLGTEAKMPTNVAAPAPD
                     LMDEQSADQPGDTAN"
     gene            complement(9427..9981)
                     /locus_tag="PLM90_01050"
     CDS             complement(9427..9981)
                     /locus_tag="PLM90_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HMZ87979.1"
                     /translation="MRNSILLAIALIIQTAVHAQIGSPFKGNEEADTIYFVELDSFTI
                     TARPLKNYNYNKYEAIVKRVYPYADTAVMTLKALQSQSFSGHHDEKQYKKELEDQLRK
                     TFEAQLKKLTRTQGEVLIDIIERNTGSSMYNILKETKGATSAFWWNNLGKMYGYDLKD
                     SYKPTNNPTLEEIIVQYEKKYKGK"
     gene            complement(10017..11165)
                     /locus_tag="PLM90_01055"
     CDS             complement(10017..11165)
                     /locus_tag="PLM90_01055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HMZ87980.1"
                     /translation="MFSSLIGALLTNGLALSMGCSNGESSIPVKPVESPDTLVTFTIA
                     AVGDLMCHSTQFNYAKTGVDSFDFTPSFSLVAPILQQADLAIGNLETTLAGKARPYSG
                     YPMFNTPDQYVRAVKQAGFDFLVTANNHSNDSGDKGIVRTISILDSIGLGHTGSFASA
                     NDRDSIRILDIKGVKIGIISYTFSTNGLPLADGKPWLVNMCDSALIKKDMAAGRAKGA
                     ELMIVFYHFGEEYQRLPNAYQKQFVQHAIDCGADVVLGSHPHVIQPMEVVAGRRSNVD
                     SVFVAYSMGNFISNQKDQYTDEGVIMQLTFEKHLPSGKIRLAAHNNYVPTWVYRGVAD
                     QLKMHIIVPVSAAPGNDTKAIYLNDAYQSSISEAKKHTDAILHTVVPK"
     gene            complement(11193..11390)
                     /locus_tag="PLM90_01060"
     CDS             complement(11193..11390)
                     /locus_tag="PLM90_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87981.1"
                     /translation="MLLKQTFRPLRYTTLLFLLYVANISLKSQSKGNGSMTQFYIVTI
                     VFTVLILAAVISIFISKKKKK"
     gene            complement(11544..13076)
                     /locus_tag="PLM90_01065"
     CDS             complement(11544..13076)
                     /locus_tag="PLM90_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HMZ87982.1"
                     /translation="MNRRSFLRNTLAGTTAIAVTPAAFLASCSDGNQDILDDVLFGTV
                     GGTTVPTPPFVSPFNTLDTQVANGSTITANRNFYTFAGISGTFCYGYSESVWGPTLTA
                     NTGDNISLTLESNLSSFGTNLHFHGLTLDASQEDGVENGVASGASKSYSFTVNNRAGM
                     YWYHAQYQKLGGDQLGLGLGGLFIVSDSEEDALNLPNGNLRSTTISLQDKRFNEDNQM
                     LYGPNKAEYLTGYFGGTILVNGRIGVFKNVSSRFHRLRIINGSTARIYNLSISTTDGT
                     ELDFNIIGNDGGLLKSLGGGIKQVLLAPGERLDSIIDFSTLSVGTEVLLSSNQFNAAG
                     DYQGVTGFDIMKFVIDTADDETWSLPDDLSDITTLTEGDVTGETRNFDIANANISKDE
                     ATNNTDNMHTINGDAYDAGTVNFTVNAGTTEKWVLDNTGGSEPRVMHIYGVQFQVLDR
                     TGGRGSVKQWEKGWKDTILLLPDEVVTLLVPFTTAPGVYYLASTNQEHADTGLINRFE
                     IV"
     gene            complement(13233..14159)
                     /locus_tag="PLM90_01070"
     CDS             complement(13233..14159)
                     /locus_tag="PLM90_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87983.1"
                     /translation="MPSKKILIPVIAVLPWISACNKDVTIFADVHFSFAFDSTQTRLD
                     NLGEPSEVPAGHGSQSPEMQLMSIHYIELTTGSNVQLGDGAVLYKAPETLAGGAQAIL
                     FDSSILASEGSDFLQIKLERLAPGTYTYLRASVAYQQFGISYDLHNVPIYGDVLDQDG
                     VLASFLGYNTYINDLHIQNLTTSVNANKEQGFWGFETLFSGDLSAYNAIYTGQAPEGA
                     TTVVNPINATSPIPAGSCVVTGMFAEPLVITGDEVQDLYITFSFSTNNSFEWIDSDTD
                     GKWDIDVLDAGATESVVDMGIRGMIPSWEWRD"
     gene            complement(14265..16541)
                     /locus_tag="PLM90_01075"
     CDS             complement(14265..16541)
                     /locus_tag="PLM90_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMZ87984.1"
                     /translation="MVSNSKLWKWVAGLKPVYVIAVFSLLVLLAVFFTLQLRFSYDYE
                     DFFPENDPDLAFYDAFRQQFEPDDNFLLVGMQPENGLFDAKFLTSMDSATNAVGRIEG
                     AERAYSVTNFRYFVKSPFGFIDYPAVHPDDSSRFESDSLRLAADVRVVGKLVSEDFST
                     VVILIKTNDSLSQQKAKSFVADVRAAMHDAGIKDYHLLGKANFEVELVEIQKKEFLIY
                     AMLSIVLVTIVTFLMFRKIWCVVLSVLTVAISLILFTGILGGLRIEQNVMSTLYPIVI
                     IIIGISDAVHFIGKYITELRKNNNRKVALFRTLSDIGFATWLTAVTSAIGFLTMLTSN
                     VPPIKDFGLYSALGVLVVYAVVLFFISPVLTLFRLDQLDAKTDGEDKKWTAFINAIYT
                     LGIRRKRRILIIMSAAMLIFIYGVTQISTNIHLEAGMPKGAQVTTDFHFFEEHFNGFR
                     PLEIAAEAKGKHLITEPAIIRDVEKVETYASSFDLVNGLQGMTMPYKSMHRAYNGDIA
                     SYYRLPENDSILYGYQKLIDKYATREMHALISSDKKAGRISGFLSDVGTDSIRMFQTD
                     LKKFIEEKTDTSAVKFTITGTGVIFDKNTQYLRKNIISGVLLAFLCIGIVMAYLFRDW
                     RMVIISIIPNILPLIVCAGIMGLLGIELDAPTSIIFGISYGIAVDDTIHFLSKFRIER
                     QKGYPLEIALQNTFQETGKAVFTMSAILFFGFMILMLSSTSVTFNIGLLTGVTLFTAI
                     WPDVFLIPILLRMMKTKL"
     gene            16726..17115
                     /locus_tag="PLM90_01080"
     CDS             16726..17115
                     /locus_tag="PLM90_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87985.1"
                     /translation="MKQLLQKLTFSLLLVSAVTFMASAQTPDRGNATIDANMVIHLDE
                     TQPLVADYSFSFSAIAFKNEAAATRFFGLCRDNIVSYNLDWAAKTATVHIALEYMEPR
                     GWDIVKYNDYFSKLAERYRTTLSVVNE"
     gene            17148..19334
                     /locus_tag="PLM90_01085"
     CDS             17148..19334
                     /locus_tag="PLM90_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ87986.1"
                     /translation="MQSRIIAVVAMVVFSLFTINSATAQIVGANVFLKGNYVEVGINT
                     CGAYGTPSAPPAGYHPTEGGLGFVADWESDGWGSGTPVYCGDYFVPGSPVEGWQLQIG
                     PTWWTNTDQSCGGSDIPGDVSDYTYAGGVYTGVWTGNLTTGTTDLDITQITTLPEDAL
                     YFVTRILLCNNGADPLNNVYYKRNVDPDNDQPWSGDFTTDNVIVYQPPTDEEALVTSE
                     GLTYGCFLGIGARDENARVSYGNFGTDDGSPKAVWDATGGYVGTGSEVGDIANSISFY
                     IPVINPGECKCVAFAYILNVDDLEAALEATVTYNLTADDITVASGDTVRTCNPGDSVE
                     LSILGADDYFWTWTPTTGLSTDTGISVIATPDVTTTYTITGVGGFCGDATLQVTVEID
                     THEFADAGDDVEICLGDVTTLTGDGGPYENIYAWTPAADLGCADCATTTATPTTDGVF
                     TYTLTTYDIYNCPATDEVEVTVHPLPDVNAGPDKDLCPEGAVDLEATGAIEYTWSPSD
                     GLSCDDCPDPTCTVDENTTYTVTGTDANGCVATDVINVNVFPNLEGIMTVDPTEITID
                     VYQNETAQLNAYGAEIYQWSPPMGLNNVNISNPIAQPADTTVYIVTGVDANGCTDVDT
                     VTVNVIGSLEIGVPTAFSPNGDGTNDFWFPMISGSGCVESWTVFNRYGEVVFEGNGFC
                     PGEVTQGWDGTYNGELQGIGTYVVIIKAATTLLEDRFVQGNFTLVR"
     gene            19440..21356
                     /locus_tag="PLM90_01090"
     CDS             19440..21356
                     /locus_tag="PLM90_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ87987.1"
                     /translation="MKHLHRLLPLLILLTCTQMQAQIVGANAFLKGNFVEVGINTCGA
                     YGSNGAPPAGYHPNVFSGLGFVADSDMDGWTTGTPDYCGDYFVPGSPVEGWQVQIGGT
                     VYTNTDQYCWTSEIPGDITSYDYTAGIYTGIWEGTIAAEDVTITQTTTLPENARYFVT
                     RILICNDGPTDLTDVYYMRNVDPDQDQPFGFSFMTTNSVIFQPPVDPDALVESVGDVY
                     GCYLGMGARDENARVTYGCFSTTDGTPAQAWSGASVCAWSSGYSLSGTYYCDCATQVT
                     FKIPLIAAGECQCVAFAYILDADDLDEALEATSSIGVAADGVDISTSGITLICPGDSV
                     ELNIIGGEDYDWTWTPTTGLSSGTGDTVVASPDITTTYLATGVGICGTIEREVTVEVP
                     PPPIANAGPDMSLCPSDTIHLAGSGGVHYQWTPPVYLDDDTLSNPAVMDPLTDMYYQL
                     VIWDANGCTDTDDVMVTLYDLPIVDAGDDQYMAEGGFAQLTASGAATYVWTPEDFLTN
                     ALIYNPIAQPEDTTMYYVVGTDANGCENIDSMIVYVLDQTHIVVPNAFTPNGDGLNDT
                     WKPVLIGIGEITYFSVYNRWGDLLFESSDPTIGWDGTYQADEQEIGNYIVMVKGLDGF
                     GQPLTKSAMVLLMR"
     gene            complement(21508..22353)
                     /locus_tag="PLM90_01095"
     CDS             complement(21508..22353)
                     /locus_tag="PLM90_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87988.1"
                     /translation="MVHSYAQPLQIINSTTKPLLTNYYDAVERADTLKALHALNGLIN
                     EDPDFPYYYIARASIHIALGNIPQDSILIDYERAIERAPDDITVLSQQAVYTGQFHSD
                     NMRIIAMNDRLSLVQLDSLNYNHYEACNNYVLSKDEAFITAFYQQATRAMFQMVTMYP
                     DSSDTWYHLANVISWNYAFNGNSEMLLKALSYYDKAIQLNPANTVFYIARATLFAKSK
                     DWENAIEDYKQAMSLSSRADWFLELGLLYEHIGEKQKAKNAFNTGLQFYPDNYLLRRA
                     KKQLH"
     gene            22554..24587
                     /locus_tag="PLM90_01100"
     CDS             22554..24587
                     /locus_tag="PLM90_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ87989.1"
                     /translation="MLHAQSLLTCGTDIERSKLIADHPEILDREAIFDALAAKKFAEV
                     ARIEGDVLVIPVVFHVVHDYGPENISDAQIFDAIEHINADFSATNTDFAATIPEFAGI
                     AANCQIEFRLAQRELSGACTNGITRHASTRTYEGSDAAKLGGWQSGKYLNIWVVKDLT
                     NGAAAYAYYPTSIAGLMYTVDGVICRYDYVGTIGAAGSYAAHVLSHEIGHYLNLQHVW
                     GNSNAPGVACGDDQVPDTPMTEGWTTCNIYGNTCTAPIDNVQNHMEYSYCSTMFSEGQ
                     KSRMYAALESDDAMRNNLPTEENLLLTGTNDGYVASTCAPIADFYTSQRYICAGESVT
                     YHDVSYNGEVTSRVWTFEGGSPASSTDIAPVVTYATEGWHQVILNVSNGVGTDTKTWN
                     NYLHVAPAVSIYDENYFEDFNNQTEVENDWILYNKYEDPYEWKWRGNNGYYNTGCIWL
                     NSRFGPDLESDVAISPMYDLSSGLTDNLFFKYSTTSYGDNEDDYTMSLKIYYSINCGD
                     SWIYMNKVTGADLITSYGGSSNFFPQYPDQWAAAAFNLPEACKTDHVQFKIEFLYNPY
                     VNNVFIDDFNFLSGVLNTTEQQASVALKVYPNPVHADGSSTIHYSLTSSNMVTISISD
                     LAGRTIQVIGNAQEQEGSHFITISPKELGLPSGCYFVQIKAGGQLVSDKLIVI"
     gene            complement(24584..25021)
                     /locus_tag="PLM90_01105"
     CDS             complement(24584..25021)
                     /locus_tag="PLM90_01105"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HMZ87990.1"
                     /translation="MIEIAQIPLILEEIPPKGIHIFIHGKIQRKTIRFLLDTGASKSV
                     IDQDFANRMFPSARRIETEHLTTGLGANIPNAVFLKLRGIKAGKYHLKPITFAVIDLS
                     VVNQAYTSAKLEPVVAILGGDILNKYRAVIDYRQRLLQLLVED"
     gene            complement(25088..26029)
                     /gene="mdh"
                     /locus_tag="PLM90_01110"
     CDS             complement(25088..26029)
                     /gene="mdh"
                     /locus_tag="PLM90_01110"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMZ87991.1"
                     /translation="MSKVTVIGAGNVGSTVANVLAHRDFLNEIIVLDIKEGTAEGKSL
                     DSWQQAPIDNYSTRVTGVTNDYARTADSDVVVITSGLPRKPGMSRDDLISVNAGIVKS
                     VTEQVVKYSPKAIIIVVSNPLDVMTYCAYLTAKIDSSRVFGMAGILDTARYRAFLAEA
                     LNVSPKDIQALLLGGHGDTMVPLPRYTTVSGIPVTELIDMDKLNAIVQRTKVGGGEIV
                     NLLGTSAWYAPGAAAAQMVEAIIKNEQRIFPVCAWLNGEYGLKDIYLGVPCKLGRNGI
                     EEIIQLKLDASEMQLLNDSAKAVREVMDVLDRMNAAN"
     gene            26174..27613
                     /locus_tag="PLM90_01115"
     CDS             26174..27613
                     /locus_tag="PLM90_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HMZ87992.1"
                     /translation="MKKLLLIAAVGLLGGVLRAQTAPSGYCGTLQTESDLTWLRNYQA
                     TYVPGELERGGTTYYIPTKVHIVGTDDGSGYYNLSYLYQSFCELNEHYAETGFIFYIY
                     GDLNYVDNTDYYEHDWFDGSNMMEDYNVNNLLNVYFVSDPAGNCGYFSPSDDGVAVAK
                     SCAMPGGTTIAHEFGHFFSLPHTFYGWEWGTPDDNDQEWVNGANCHSAADGFCDTPPD
                     YLPYRWNCPGPLQTDPHGETFYSDGTFYMSYSNDACTDKFSAEQQDAMISNLLGPRNN
                     FLDYPTPAYADLTEASTLITPEDAAINQFSNYLVFEWTPVTGATQYHLQLAYNASFSA
                     IAKDVVTTNTFYEATDLQMDKKYYWRVRPLAPANTCASPSNARSFTTGTELNSVANSE
                     IFDHLAVYPNPALVGQNLTMEYRSAESFRADISLLDMTGRELTSTNYQINQGNNTLDV
                     AVPAVSSGMYILRITAENRIETISVMIRN"
     gene            complement(27651..29012)
                     /gene="tilS"
                     /locus_tag="PLM90_01120"
     CDS             complement(27651..29012)
                     /gene="tilS"
                     /locus_tag="PLM90_01120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HMZ87993.1"
                     /translation="MDKLSTHQLHKFLHTLPGFTQQARILLAVSGGVDSVVMTYLLAS
                     ANYTIGIAHVNFQLRDGDAEKDEAFVKNLATSLNVPFFTTSVDTKKYASANKLSIEEA
                     ARNIRYQWLEEIRIQNNYDFIATAHHLNDHAETMLMHLTSGTGLQGMHGIRARNGNII
                     RPLLFATREQIETYASEHQLTFCTDVTNNSDVYLRNAFRKQVIPFLQQTNPSFLQTMA
                     TNAQHFREAHLLADERAVMLLHKWLKTTPTAISLPIKPITNHPAASTLLHTWLAPFGF
                     THAQITELATQQHVSGKQFLTNTHRLIVDRKHFLLTSYPQVNANIQIIENPSGSYHIK
                     NGKLKIEKMAMHPNINLQASNNIAYFDADKIEFPLTLRVWQKGDYMYPLGLFKKGKTK
                     PGKKKISDILTDARIAIPEKENTFVLCSGEYILWLIGIRSDERFRVSNNTTNVLKISM
                     LPD"
     gene            complement(29016..29345)
                     /locus_tag="PLM90_01125"
     CDS             complement(29016..29345)
                     /locus_tag="PLM90_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ87994.1"
                     /translation="MERIPQLLSYLNDNPGDAFLNYALALEYIKIGDKEEGRKYFEHL
                     VNIQPDYVGTYYHLAKLYISMKLPEQAEQCYQSGIAVAQKLGDQHALAELQNAALNFR
                     LGLDDED"
     gene            29475..30215
                     /locus_tag="PLM90_01130"
     CDS             29475..30215
                     /locus_tag="PLM90_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMZ87995.1"
                     /translation="MKLLVCISKVPDTTAKVAFTDGNTRYNENGVQFIINPYDEWYAL
                     VRALELKETLGGSVTVINVGKADNEQIIRKALALGADDAVRIDMDPADAWQVASQIAQ
                     YAKGGGFDIIFTGKETIDYNGGIVGGMLAELLDLPYVALAAKLDVDGTQLNIAQEMEG
                     VKQLIKVSMPCVVSAQKGMAEARIPNMRGILASKSKPVQVIPASGDAAKSPVKVFVMP
                     AEKQGCKMISAENPEELVQLLHNEAKVI"
     gene            30238..31188
                     /locus_tag="PLM90_01135"
     CDS             30238..31188
                     /locus_tag="PLM90_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMZ87996.1"
                     /translation="MQLVLVELINGKCKKTALEAVAFAAKMGETTALALGTASADTLQ
                     ALGAYGIGKVLHINNEKLNQVYSKAYTKAIAAAATHVGADTVILSHNSTGRMVAPRVA
                     VRMEAALFAGVNELPGNDTIRKAVFSGKAFADLQMSTGKKVISLNPNSYKAVAGTGTA
                     SVESFSVDFADSDFDYAITGVEDEAGGHVPLPEAEIVVSGGRGLKGPEHWGIVEDLAK
                     ALGAATACSRPVADIGWRPHHEHVGQTGITIKPTLYIAVGISGAIQHLAGVNQSKVIV
                     VINKDPEAPFFKAADYGIVGDAFEVLPRITEAVKKLHAGA"
     gene            31247..31879
                     /locus_tag="PLM90_01140"
     CDS             31247..31879
                     /locus_tag="PLM90_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HMZ87997.1"
                     /translation="MQKVELEITGLSHTITQSQSYAVLLSEVHGQRKLPIVIGAFEAQ
                     AIAVAIEKVVINRPLSHDLIKNMFTVFQINLREVLIDNLQEGIFYSKLICEKDGQEFD
                     IDSRTSDALALAVRFNCPIYTYEFILDSAGAIINEPEPDDDNEEDENEEEDIDALEEL
                     SPTGFSSESGSDLTNMSLKELNDRLREFLEHEDYESAARIRDEIEKRKKP"
     gene            31882..32262
                     /gene="crcB"
                     /locus_tag="PLM90_01145"
     CDS             31882..32262
                     /gene="crcB"
                     /locus_tag="PLM90_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HMZ87998.1"
                     /translation="MSFSTIIWIFLGGGFGTLVRYGIGTQVSGLSKSNFPIGTLAVNL
                     IGCLIIGLIYGYFEKHQETQSDWKLILTIGFCGGFTTFSAFSIDAIQLVKGGNLLLAI
                     LYIFSSVFIGILLSILGWQIAKNY"
     gene            32289..33635
                     /locus_tag="PLM90_01150"
     CDS             32289..33635
                     /locus_tag="PLM90_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HMZ87999.1"
                     /translation="MVESIFRGLLGVAFLIGVCYLFSNNRKSIDWKLVGSGLLIQFVF
                     AFVIIALPDITEQHFGYRFDWFERIFQGMANFFVMVIGFTNAGANFVLGDWPSVTQVS
                     DGLTGQVFTVGFIFAFKVLPTVIFFSALTSLLYYLGILQKIVFGFAWVMSKTMRLSGA
                     ESLNAAGNIFLGQTEAPLLIRPYLEKMTKSEVLCVMVGGMATIAGGVMAAYIGFLGGD
                     DIEKQKMFAMHLLSASIMSAPAAIVAAKMLFPQKENVDTELKVNKERIGSNVIESIAN
                     GTTDGLKLAVNVGAMLLAFTAFMAMFNAIFLGIGNMLSINDNIAAATNNTYSGLSLDY
                     IFGIIMAPVAWLLGVPSSDMLMVGQLLGEKTIINEFVAYSSLGNMKSMLDEKSIIIAT
                     YALCGFSNFASIGIQIGGIGALAPNQKTTLSQLGLRALLGGTVACFFTAAIAGMII"
     gene            33646..33903
                     /locus_tag="PLM90_01155"
     CDS             33646..33903
                     /locus_tag="PLM90_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88000.1"
                     /translation="MQEFTQWAIFMGFIALMAAILYAYASGWVNSPRTRRESFKEWQK
                     LNGGVVRMMSLLVILVCVIVCIYKYNDIKAAIDAPPTLQTE"
     gene            complement(33907..34851)
                     /locus_tag="PLM90_01160"
     CDS             complement(33907..34851)
                     /locus_tag="PLM90_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88001.1"
                     /translation="MRAFTFFILQLSFFLIYAQPCDDRNTEYIPIADLGSSTFQGYQG
                     GLYPNGSNAIPMQHFQEGLSVASHIKPLNAEGKEDSISGKIGFLILGYSTAAMTGRFF
                     RDVVAIKSPDTKLQVVIGAQGGRDINSMTDSLSSYWDGVDSALHSNTITAAQIQLIWI
                     STGDILTYSLPFPQQSLQQVDKYALVLKQIKLQYPNARIVFLSDRPFAGYIGVNGMGP
                     KELAEPTAYFHSWTVKWVIEHQMNNDAGFTKEEIPFIDWGPTLWTNGSKGDKQGYTWN
                     CDDAGKGGIHASSKGRMKEATRMYVYFSNHPYTEQLFK"
     gene            complement(34854..37313)
                     /locus_tag="PLM90_01165"
     CDS             complement(34854..37313)
                     /locus_tag="PLM90_01165"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMZ88002.1"
                     /translation="MRKCLIYVCLLIILITRLSAGTISGHITDTTGAPLPFCNVYLSG
                     TTYAVPANAEGFYQLTAPNGEYVIVFQFIGYSRKEIPITISDKPLMFDVVLQEEMTEL
                     QTIVIGPDHEDPAYAVIKQAIERRADHLKEVNAYSCDVYMKGLQKLNEAPDQIMGIKL
                     NTVFDVDSNNTGIIYLSESVSSFNYKYPDQTKEIMKASVVSGDKNQFSWNDAAGMQMN
                     FYQNLETLQGFSQRGFVSPIADNALFFYTYHLLGNTFENNREIFKIQVTPKRSGDPAY
                     TGVIYITNDDYRITGLDLTLTKQNGIEFLDTFNVTQEYYYADREHLMLLSNKFSFNYG
                     MFGIQGEGYFHAYYKNYTINPIFDKHFFDGEVIRINAGANKQDSTYWAGIRPMQLTKE
                     EQLDYHEKDSLAILKESPAYQDSMDHIFNKFSPAVLITGYNWRDSKSKSMISTNNIFD
                     LLQFNTVENYVIQPRLTYTHWYEDNRRFEIGTTLRWNTATNGALISMQSRYLLDPVSQ
                     EKLSLLAGSRMQQFNPMGISPLVNSLYTLLLEQNSMKLYAEDFIQFDADREMYNGFYF
                     GIGAYYGKRYRQYDAINTEPWIDDQSKYFSNNNDFVIHNATVFEWGPMSDILMLHLHI
                     KYVIKQTYILHPDEKFINEPKYPVIELDLRSANVTLNDELDQFNKIHLSVFDRLPLSL
                     VGNLEYIGTATLQFADYEQLIVDKIHAIGNETNVSRLQNETFYLLPYYYTTASDYMFT
                     GHVSWHTEGFLFRKLPLFKQLKMEPVFSAHVLTSDTMPFYTEFTFGVEHLFKIIRVDL
                     AITPMHDDFIPPARLFIGFGF"
     gene            complement(37352..38587)
                     /locus_tag="PLM90_01170"
     CDS             complement(37352..38587)
                     /locus_tag="PLM90_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ88003.1"
                     /translation="MENNNHHIDRIFKNGLKHYSAQPSADLWNRIADELDNGMPAKSS
                     RAQWYRYAAMFLVLLSVALISVPGNRHTPPAIFAFDNNTGNSYAAPISHTAMPSFTAL
                     IPEITQVVADNISLRRTPSESVSFENPVQQITTADLSIKTHPKDAFIYLPAEPENETL
                     AAASQDVFDRFYNNDEPIDVMAYNYFGDDDPEADFFEAPSYKAKYRDYDMRGLYIGAT
                     GSYNQTSLLESGNVFKGERPIQPSLKFGTTKGLRVGYNINNSFGIEAEYVYNALQGQN
                     YVMSEGDDIVEKSLSLNYDLIPVVAKVKIGHISDITNKPVVLNYTAGVQYGMLREARM
                     PQDKRYEESAMELFKETDVSVVLGLEYDVFVQDNIVISAGARGTFSNDISTHIEPLND
                     YAKRNFVFGLHGGVSYVFR"
     gene            complement(38577..39155)
                     /locus_tag="PLM90_01175"
     CDS             complement(38577..39155)
                     /locus_tag="PLM90_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ88004.1"
                     /translation="MKRLRIPINIPEEQLIRQCLQGDRVSQKHLYDRHSSKMFGVCLR
                     YAPDYQAAEDILQEGFIKAFRNLERFRFEGSFEGWLRKIMVNTAIEVHRRKNSLYPIM
                     DVEHTDVEFFDENALDKLAAADLMQMIDTLSPGYKTVFSLYAIEGFSHKEISEQLNIS
                     EGTSKSQLARARYILMEMVEQRTGLKRKAIGE"
     gene            complement(39205..39822)
                     /gene="rdgB"
                     /locus_tag="PLM90_01180"
     CDS             complement(39205..39822)
                     /gene="rdgB"
                     /locus_tag="PLM90_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HMZ88005.1"
                     /translation="MGAITDPVRIKMSDLIFATANENKVKEVQLMMPSGFAIKGLRDI
                     GCLEELPETTGTIEGNSRQKAMYVHEKYGVDCFSEDTGLEVAALDGEPGVDTAHYSGS
                     RDPEANIRLLLMNLRNEGNRAAQFKTVFTLIIGKNVHQFTGIVTGKIAEIPTGNMGFG
                     YDPVFIPDGYSTTFAEMKTEEKAPISHRGKAFAAMTAFLTQNFWK"
     gene            complement(39786..40262)
                     /locus_tag="PLM90_01185"
     CDS             complement(39786..40262)
                     /locus_tag="PLM90_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88006.1"
                     /translation="MLEFGGAMVDEPVFLVSDATKLTISPTKANDEGLDLSNEVHSLF
                     LFKPSSGNRLSPDAMIALDKMIAACGLTKENAVALPFLGQGNLHFNRLASAGYVKKLI
                     LLGITPDEIHLFVDSQYYNIFNFNGIDILISDNIEGITKEQKQQLWARLQTLFGLK"
     gene            40328..41407
                     /locus_tag="PLM90_01190"
     CDS             40328..41407
                     /locus_tag="PLM90_01190"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HMZ88007.1"
                     /translation="MDTAINTTFPIEIHKSPVSHISTVDFDQLQFGKVFTDHMFEADY
                     IDGKWTNCTIRPLSTLQLHPATSALHYGQAVFEGMKAFKTTEGKVMLFRPDANFRRLN
                     ESCVRMAIPELPEELFMDALFQLVRIDADWIPNTDEGSMYIRPFVFATDDFVGIKPSD
                     TFKFIIFCCPVARYYSKPVRVYMQEQFIRAFPGGTGAVKAAGNYGATMMPLMEARKRG
                     FDQMLWIDGVTHNRLQEIGTMNVFVQIDDTIITIPTTEGTILKGVTRDSCIALLRDGG
                     YKVEERDVTIQELIDAHRSGRLHDAFGTGTAATVAPIGVIGYKDEEYELPPVESRKAS
                     AWLKHELWAIRKGIATDRFDWMWEV"
     gene            complement(41468..>42658)
                     /locus_tag="PLM90_01195"
     CDS             complement(41468..>42658)
                     /locus_tag="PLM90_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88008.1"
                     /translation="VVGNTYGAEVTNTGISITKRSSDTWTIPEPIVITGFENLNQYNE
                     FSMSASGNILLMSIEKADGMGLLDLYVSFRQADGSFGVPKPLGVDINTAGSEMTPFLA
                     ADESTLYFSSNGFPGYGNQDIFVAKRLDDSWQRWTSPVNLGPEVNTPEWDVYFSIDAD
                     GDYGYFSSSKNAITNLDIYRIQLPPELQPDNILWLKGNISDRITGQPLKAMINYESTG
                     VRTGTGTSGEDGNYALVMPGATDYHVLVSAKGYLTADTIIDLKSLLADKQAIQDFELV
                     AMQEGIVIKIPNILFKVNSAVLEDTSFIELERIARLLINNPGISVEIRGHTNGMCDDT
                     YCKSLSTKRAESVVKYLVSKGISPERLTSKGYGKSMPVADNTTPEGRQANQRVEFMIT
                     KVGD"
BASE COUNT        12347 a         8904 c         8922 g        12485 t
ORIGIN      
        1 taccaacgta aactattcgt ccatatcctt gaaaaactag cggtaataaa cgctcttcca
       61 gaatactacc gaaagaccat aacacaaaca tatttacaat cagatgaata taatcggcat
      121 gaataaaacc actggtgata aacctatacc attctcctcg ttgatgcata gcatgcggat
      181 aaaacaacgc cgagccgcgc atgacagaat tgttaaatgc cagaatactg cttattgcag
      241 ttaataagat aatgaaagcc gttaccgtta cattttccat tttttaatga tgatagggtt
      301 ctctgttgat gatggtaaat gcacgatata attgctcaag aaaaataatg cgtaccattt
      361 gatgaggaaa tgtcatatct gataagcgaa gtgtttcatt tgccattgaa taggcatctt
      421 catgaaagcc atgcgcgccg ccaagcacaa acactaagcg tttgcgattt tccagctgtt
      481 tgcgtgtaat aaattcactg aaaccaacag aactgtatgc agcacctctt tcatccaaca
      541 acactacatg gtcaccttgt tctatatatt ttttaatagc cgcaaactct cttagcttca
      601 tctcctcagg tggaagtgat ttaatgtctg ttgtttcatg ccactgaaat ctcacatatt
      661 tacccaatcg ctttacatag gttgaacatg catctgaaac ccacttttca gaagtctttc
      721 caacactgat aaaaataatc tgcatgcgga tataaaaata cggttattga agcaaagtgc
      781 taagcgtggg tgctgataaa ttatctttgg atttacgaca aaaatccttt gtgttgagaa
      841 ggacaaatag gcgatatagt atagatcaga accccccttt tccaaccacc aaactcttaa
      901 ccatgatgtc tagcagctta ttgttcctgt cgaaccatcg ggtaggggca tgcacataaa
      961 cggtgaccct tgtttttggt gtgtcgtagt aataatatct gtcgtctgac tgcatacaca
     1021 aaatacccgg ataaccattc gaaaattgaa atgaggtctt ggattcacaa gtatttaact
     1081 ccatttctgc catcacctga ttatcggcta tgttttcacc gtaaacgcga atatctacat
     1141 ccttgtcgcc ggaggaaata aaatagccat caccattatt gcttttttct ttaatagtgt
     1201 aaaacccggg aatggtaatg gtataatcga atcgttcgtt atacaatacc tgatcgccac
     1261 ttgcttgttt cacaacatca ggtgtagtag cttctttatc tattaattcc tgtagtgaat
     1321 tatcgcctcc cttttcgcca acattttcta ttgccgctgt gtcttcattg tcggcagact
     1381 ccttacatgc gtgcagcatg aggattgaaa tggctaatag cgctcctatt tttatttgca
     1441 tagcatcatt taaaatgcat cattctatcc cagcggatat tggcgatccg ctcactcaat
     1501 ggcaatgtct tatcccagct gaaccaaagt aaaaccggct tgccaacaat atggtcttca
     1561 ggaacatatc cccaataacg tgaatcataa ctgcgatgtc ggttatcacc catcatccag
     1621 taataatttt gtttaaaggt atattctgtt agcaatttac catcgatgta aggtctgcct
     1681 tgtttatcaa gcgtaactgt attgtgctca tatgcttcaa tgatgcgctt ataattataa
     1741 taactgttcg catcaatttt cacagtagct ccttttttgg gtatgtaaat cggactgaaa
     1801 tcatcaacgg aatttaacgg ttggtttgga ttattcggga ataacccaat atatcctgct
     1861 accatcctgt cgaagtgatt taaaatcaca ctttctactt gtttattttt cgcaagaagt
     1921 ttcgaatccg caatactcat attcgcagtg atcataatct tattaggtgt cacgttaacg
     1981 gtatcttcga aatccattga attgagtttc atcttcaata aaataccggc tgcggcatca
     2041 acacccaaac ctgctttaaa tgtgacatca tatgattttt gctgctcagg tgctacatat
     2101 gctttctcgc cattcacata cagaattcca ttgctcaccc acaaagaatc tcccggaagt
     2161 cccacacaac gcttgatata attgtcgcgc ttgtcaacag ggcggtaagc aagctctcct
     2221 gaactgcgat atgctgccga tactctgttg taataaaatg aaaacggttt tggacgattt
     2281 cctagttttg cgtcattaat ttgaaggtta gtagcttcat tcatgagcag tgaatagtaa
     2341 ctgttttgct gttcaggcaa atacactgta tcacccgcgg gaaaattaaa aactacactg
     2401 cttccgcgat gaatattttc aagcccggga aatcgcatat atggcatctt gattgcatca
     2461 gaatatgctt tacctccgat gacaggcatt gtatgatgtg caaatggaaa tgccaatgga
     2521 gtgatcggga aacgtgcacc gtaatgaaat ttacttacaa ataagaaatc acctgccttt
     2581 aaagtgcctt ccattgaagt tgtcggaata gtataagctt caaagaaaaa tgtacgtatc
     2641 aaggtagcag ctactacagc aaaagctatt gcatctgccc attcacgtcc aactgatttt
     2701 cgctccgatt tatgaggtcc gatgtaacgc acatcttcat caaatcccaa cttgagaaaa
     2761 tagatgtatg gaatcatcat tgatccaaca tatgcccaaa aactctcctt gccaaaaact
     2821 ttcgccagct catttgacat gagtaaccat accaccacat tcaaaaccgg tatcatcagc
     2881 atgatgaacc acgatctgct cttaccggta atctttacgc atatccaggc atttaaaaaa
     2941 ggaactaaag ccttccaacc ggcttcacct gctttctcaa acattttata caagcccgca
     3001 agtgtgccaa ctattgtaaa aatgaaaaat atccaataac caataggaat gatggtctcc
     3061 atgcacgtat tttattgtaa agtaaatgac taagcttgct gcaaagctag aagatttcga
     3121 tgaatagcgg tttaatggcg gtaaacctcc ctaacatcag gggattcttc tgtatatctc
     3181 ataagtaaca ggataactga accatctttt gtccaatgta taatgtgctt ttatactgtc
     3241 atacatgggc atagtgacac tgttcatcac atttgaatat atcaatagct ctgaagttgg
     3301 gattgagtcg ggagaaatgg cattgtattc agtatcgtct tcaactaaat ctgtcaaggc
     3361 aaaggatttg tattcaggaa aatatgtgcc tacttcgctg tcgtcaatat gatgtgcctt
     3421 cagatatgtc tcaccctccg ctctgaatcg gaaataactc aaatgcgata aggacccgcc
     3481 ggaccaatca tattcaaaaa tagatgtgcg catttgcgga taagcgataa aatgctgtgc
     3541 aataaataca catgcagtca tcgcaacaag acctccccgg aataattgac ctcccgcttt
     3601 aacaaaaaat ggtgcagatg ctatgagtat taacacatgc aaaggcatca tatacctgat
     3661 taaaatggga ccccgaaagg gtagtgtaaa tagggcaata ctgaaaaccg aaatagcaat
     3721 taatccgatg ttgctcgtaa ttgaaaatcg attaagcaaa gcgctcccac gcataatcat
     3781 gactaccacc ggaatcacca ttccgaattc aatacacctt agcaagaact caaatccttt
     3841 tcctgccaat agttttccat caaccagttg cctatgttcg ccataacgct catctatagg
     3901 cactatccac cacctgctga ctgcatgctg ccatgtgtaa taagcaatga taggagtcaa
     3961 ggcaatcagc gtcctcaaag cgatcatcat aaaccaccta aaacggaact gctctcccga
     4021 ccatacataa atacacattg cgaaagccca aatccaccca cgcaaactta ccagtggtaa
     4081 taagcctaaa ataatcatcc acaccaccca ctttttgcta attcccgctc ttaaacccat
     4141 gaaaaacaag ccacagagca taatatcagc actcattccg gccgtttgcg cccaccaaac
     4201 cggataacaa agcaacaaaa gtgtgaaaac cgccaaagta gccttttgca atttcacccg
     4261 tgcgaagctt atcacctgcg aaaacataaa caactggcaa agtatcatca gcaggtgaac
     4321 ggagatgaga tttctaccaa aaactaccca aaaacctgca gtcaggagag gaaacaaagg
     4381 gaaatggccc gtatttaggt catccggaag gataggggag tgtataccat gttcatagat
     4441 ataggaggcc ggagcactaa caagtgtcac ctgatcgcca taaaagggca aattcatggt
     4501 cattacccca aaagcaaggc aaaacaagac acccgctcgt atccatacct gtggctcctg
     4561 aagcaaggtt acaatatctc gacccttttt tccgtctatg ccctctatat tgcccttgga
     4621 atgtgacaag ttgtcagttt ttacgtaaat ttgcatcaaa cttaagcaga aatgtacgat
     4681 aataatccta ccctcgaagg tttggaccaa cataaactgc tcgatgaaac gcgacagggg
     4741 gaagcattgg gcaacgaagc aatggctgaa gcggcaaccg gaaagcgttt ttacatcgaa
     4801 tcttacggat gtcagatgaa cttcagtgat tctgagattg tagcctcaat attacatcaa
     4861 cacggtttcg gactgacaaa cgattataac gatgccgacc tgatattact gaacacatgt
     4921 gccattcgcg acaatgcaga acagcgtgtg cgcaacaggt tgaaggagtt gggagcggtg
     4981 aaaaaacgca aaccggaggc gttgattgga atgctcggtt gcatggcaga gcgactaaaa
     5041 gccgacctgc tggaagaggc taaactggtg gacatagttt gtggtcctga tgcgtatcgc
     5101 gacctcccta tgcttgtgaa tacagccgat gatggcgcta aggcagtaaa tgtgttgctc
     5161 agtcgcgaag aaacttacag cgatatcagt ccgttgcggc tcgatgccaa tggcgtgtgt
     5221 gcttttattt ctattatgcg tggctgtgac aatatgtgtt cattctgtgt tgtacccttt
     5281 acgcgcggac gcgaacgcag tagaaatgcc ttttcaatac tagccgaagc ccgtgatctc
     5341 tttgaaagag ggtacaagga agtaaccctc cttgggcaga atgtcgattc gtataaatgg
     5401 gaaaatcccg aaacagggaa agtggtgaat tttgccgaat tgatggcaat ggttgcctcc
     5461 gtcgattcaa cgcttcgcgt gcgcttctct acctcgcatc caaaagatat tactgatgag
     5521 gtgcttcata ccatggcgcg attcgataat atctgtaatt atatccacct accggtgcaa
     5581 tcgggtaact ccagggtact ggagcttatg aacagaacct acgaccgtga atggtacctc
     5641 gatagaatca atgcaattcg caacatagtg ccggattgtg gtatttctac ggatgtaatt
     5701 accggatttt gcagcgagac tgaggaagag catcaggata ctttgagttt gatggaaatg
     5761 gttcaatacg acctggctta catgtttttc tacagtgagc ggccgggaac ccttgctgca
     5821 aggaagtata ccgatgatat tccgcacgag accaaaatca gaagattgaa cgagatcatc
     5881 gcgctgcaaa cgaagcattc gcatgcaaga aatcaagcgg atatcggaaa gacattcaca
     5941 gtcctcgttg aaaagcctag taaacgttcg gcactcgata tgtgcggtcg caatgatcaa
     6001 aataaaatgg tcgttttccc ggctacagat gtaaatcccg gcgactatgt gcaggtgcgt
     6061 gtcacttcag ctacatcagc aacgcttatt ggcgaaatgg tgggcagagg catgcataaa
     6121 gcagtttaaa aaccatggca gacgaaattc aatccataaa acagcgattt ggcattattg
     6181 gcaatgcccc gggactcaat tacgcattga gtattgcagc tcgtgtagca ccaacgaatc
     6241 ttacggtgct cattaccgga gaaagtgggg tggggaaaga gattttttct caaatcatcc
     6301 atcaatacag cacacgcaag catggtccct ttattgctgt gaactgcggt gctattccgg
     6361 aaggcaccat tgattctgag cttttcggtc atgtgaaagg ctcgtttact aacgctgttg
     6421 acgacagaaa aggctatttc gaaacagtaa acggcggtac tatttttttg gacgaagtag
     6481 gtgaactgcc gcatggcaca caggcgcgac tattacgtgt gttggaggca ggtgagttta
     6541 ttcgggtagg gtcatcgaag gtgcaaaaaa ctgatgtaag ggtagtggca gctacgaatg
     6601 tagacctgca taaaggtatt gctgaaggca aattccggga agatctgttt taccgcttga
     6661 acactgtgcc tatctccgtt cccgccttga gagagcgaaa agatgatatc cacctgttat
     6721 ttagaaaatt caccagcgat ttcagcgaga aatacagaac attagctatc acgcttgatt
     6781 ctgatgctgt agagcttttg cgcaattata actggccggg aaatattcgt gaattaaaaa
     6841 atattgccga acaggtttcc gttctggcaa cagacaaaat ggttaccgct gctgaattgc
     6901 atcagtttat cccgcaacaa cgcaatagta tacttcctat gatctccgga aagcaaacgg
     6961 gaaacgattt tactgagcgc gatctgctgt ataaggtgtt gtttgatatg aaacaagatc
     7021 tcaatgatct caagcagttt gtgcttcata tggcagaggc aaatcacctg aaaacagata
     7081 tgtccatttc agcagaaatg cacgatcagc ctacatttaa cgagctttca acccgccaac
     7141 cgcagttgca aactgaattc attaccaagc aacctgtggt tattaatccg gccaactcgg
     7201 ctgcacatgc tcacgtacat gaagaagtgg aagagagttt gtcgctgata gacaaagaaa
     7261 aagaactgat caataaggca ttgtcaaagc atagaggcaa acgaaaggat gctgctatgg
     7321 atcttggaat ttctgaacgc acactttatc gcaaaatcaa agaatacaat ctcgatgaat
     7381 aagcgcagaa tcacgtattc cttagtttgt gtggcatttc tgttgtcaat tgccgctacg
     7441 tgtaaagtgt cgtttaacgg ggctcagatt cctgtcgatg ttaaaacgct taccatcaat
     7501 tatttcccaa accaggcaac cctcgtttcc ccatctctca gccagacatt tacagaatca
     7561 ttgaaagata aatttttaag agaatcctca ttaactttaa ccgaacaggg tggcgattgg
     7621 gaattaagcg gggcaatagt gaaatatgaa agcaccccga ttgcgcctac aggacaggag
     7681 acaaccgcgc ttaatagact gagtatcacg gtgaaagtag actttacctc acgtaaaagt
     7741 gaagatgccg actggagcca gaattttatg cgatacgaag attatgaaag cgcaaaaaca
     7801 ttgagtcagg tagaaggcga tctgcttccc aagctttcgg agcagcttgc cgacgatatc
     7861 tatcaaaaat taacgcagaa ctggtaatgt ttaccgagga attcaatagc atcatcacca
     7921 acccacattc acttcaggat gtgcccctcg ccgatcttaa agtattggca gagcgctatc
     7981 cctggtgcca gccggtgcag gtgctgtatg ccagaaagct gtttttggta gaatctcctc
     8041 aatttgaaca tcagttacat cgaactgcga tattatctta cgacagggaa gtattattca
     8101 agtacatcaa cgccgaaata cctgaagtca tcagtgtagc gcctgagcct gaagttcaac
     8161 cggatcagcc tgtcatagtt gaggaacatc aggagattca gcctgttgtt gagatttctg
     8221 aaccggaact tgatcagatt agtgtagaag agtttgatcc tgccaccgaa gaactcattc
     8281 aggaaatgat tgaaagcatt cctgaagtgc atcaggaaga cgagatagca ccaattgaaa
     8341 tgatgagtga ggaaacgatg gaattacagg aagaagaaat tgaagcagaa agcatgttgc
     8401 aggaagaaat ggatctcgaa aacctggatc atgctgtgat caatgatgat atggaagctg
     8461 tcgcctcaaa ccctcttccc gacgagcgta tcgagccgga tgaggccttt atatttgaga
     8521 tgccggctta tgatatcgag acagaattgg gcactctgga tgaggccgat aagtttacac
     8581 ttccaaacat tccaccggaa gtagccgcag aaaagaatac agaagtgttc accgattcat
     8641 tcaccggctg gcttaatcgt ctaggtggaa gccctaccgg caaagtagtt gagcaaaagg
     8701 cttctaatgc gcctgtaaaa gtatacctgc gcaaacagcc aacgccggga tccgatcctg
     8761 ccggcagcgc ccatgaacgc ctgatgaatg aacaatatgc agcagaactg gctcgtaaaa
     8821 gcattcaaag cgacgatgga cttatcagcg aaacctatgc ccgtatcctt gtaatgcaag
     8881 gcaaatatca gcgtgcttca gagatgtatc gcaagctaag tttgctaaaa ccccaaaaaa
     8941 gcgattactt tgcagcctta attgatcaga tcaacaaaaa gataaaataa tatgttgttt
     9001 accgtaatta taattattgc cattatcgca gccgtattgc ttggtttggt agtattgatt
     9061 caaaacccaa agggtggtgg cttatcagga acatttggcg gtgccgctaa ccaaatattt
     9121 ggttacaaac gcacaacaga tgatatagaa aaaatcacat ggattctcgt agctgttgtt
     9181 tttgttctct gcctcagtgc ttctgcattg aaaccggtag aaacagttac acaacaaggt
     9241 ggaggacttg gaacagaagc taaaatgcca accaatgttg ctgcaccggc tccggattta
     9301 atggatgaac aatctgcaga tcaacccgga gatactgcga attaattgat caacaatttg
     9361 aagagataac aaaaagcact tccttggagg tgcttttttt atgccattca gtggaaataa
     9421 tgccttttat ttccctttgt atttcttttc gtactgcact attatctcct ctaaagtggg
     9481 gttgtttgtt ggtttataac tgtcttttaa atcatatcca tacattttac ccaggttatt
     9541 ccaccaaaat gcagaggtag cgcccttagt ctcttttaaa atattataca tcgacgaacc
     9601 ggtattgcgc tcaataatat ctatcaatac ttctccttga gttctggtta attttttgag
     9661 ctgtgcttca aaggttttac gcaattgatc ttcaagctct tttttatatt gcttttcgtc
     9721 gtgatgcccg ctaaatgatt gtgattgcaa tgctttgagc gtcattactg ctgtatcggc
     9781 atacggatac acgcgtttta caatagcttc atatttattg taattgtaat tctttagcgg
     9841 tcttgctgtt atcgtaaatg aatcaagttc aacaaaatat atggtatcag cctcttcatt
     9901 tcctttaaaa ggacttccga tctgcgcgtg aacggcagtt tgtataatca aggctatggc
     9961 taataaaatg gaattacgca tgataattga gttacaaatt gcgtgctagg tttaatttat
    10021 ttcggtacaa cagtatgtaa tatcgcatcg gtatgttttt tggcctctga aatacttgat
    10081 tgataggcat cattcaggta aatggctttg gtatcattgc ccggagctgc tgaaaccgga
    10141 actataatgt gcatttttaa ttgatcggca actccacggt atacccaggt aggaacataa
    10201 ttgttatgcg cagcaagcct gatttttcct gatggaagat gtttttcaaa agtcaactgc
    10261 atgatcaccc cttcatctgt gtattgatct ttttggttgc tgataaaatt acccatggaa
    10321 taggctacaa agacactgtc aacattggac ctgcgccctg caactacttc cataggttgt
    10381 atgacgtgcg gatgactacc gagcactaca tcggctccgc aatcaatcgc atgctgtaca
    10441 aattgttttt gatatgcatt cggcaaccgc tggtactctt ccccaaagtg ataaaacaca
    10501 atcatgagtt ctgccccttt cgctctgccg gccgccatgt cttttttgat caatgctgaa
    10561 tcacacatat ttaccagcca tggttttcca tctgcgagcg ggagtccgtt tgttgaaaaa
    10621 gtataggaaa tgataccaat tttaacgcct ttaatatcga gtatgcgtat gctatctctg
    10681 tcgttggctg aagcaaatga tcccgtatgt cccaatccga tagaatctaa tatggaaatg
    10741 gttctaacta ttcctttatc gccggaatca ttgctatggt tattcgcagt caccaggaaa
    10801 tcgaatcctg cttgtttcac agcacgcaca tattggtctg gggtattaaa cattggatat
    10861 ccgctatagg gccgtgcttt tcctgccaga gtggtctcca gatttcctat cgcaagatct
    10921 gcttgctgca gaatcggtgc aacaagcgaa aaacttgggg tgaaatcaaa gctatccaca
    10981 cctgtttttg catagttgaa ttgggtgcta tgacacatca gatctccaac tgctgctatg
    11041 gtgaaagtaa ccaatgtatc aggactctca accggtttta cagggatgga agattcgccg
    11101 ttactacagc ccatactaag agctaaaccg ttggtcaaca gtgctccgat caaacttgaa
    11161 aacatgcttt gatgaattgc caaacggcga ttttattttt tctttttctt ggagatgaaa
    11221 atggagataa cggcagccag gataagcacc gtaaatacga tggtcacaat ataaaactgg
    11281 gtcatactac cattgccttt tgactgtgac ttcagactaa tattagccac atacaaaagg
    11341 aacaataagg tggtatacct cagtgggcgg aaggtttgtt tgagcagcat acacgacggt
    11401 ttgttctttc taaggtaatt tttattattc agaaaacgaa atcggtgaat gttagtaata
    11461 taaacgaaag gtcatttttt tgataaaaat gaaaaaaccc tccgaagagg gttttctgag
    11521 atacatataa ccatgacaga atattacacg atctcaaatc tgtttatcag acctgtatca
    11581 gcatgttcct ggttggtaga tgctaagtag tatacgcccg gagctgttgt aaatggaacc
    11641 aatagggtaa caacctcatc aggaagcaga aggatagtgt ccttccagcc tttttcccat
    11701 tgttttacag atccgcgtcc accagtgcga tctaacactt ggaattgaac accatagata
    11761 tgcattacgc gaggttcaga accgcctgta ttatccagca cccacttttc agtagtgcct
    11821 gcattcacag tgaaattaac tgttccggca tcataagcat cgccattaat ggtgtgcata
    11881 ttgtctgtat tatttgttgc ttcgtctttc gatatgtttg catttgcaat atcaaagttg
    11941 cgtgtttcac ccgttacatc accttctgta agcgttgtaa tgtctgagag atcatcaggc
    12001 aatgaccatg tttcatcatc tgcagtatcg attacaaact tcatgatatc gaaacctgta
    12061 acgccctgat aatcgccggc agcattaaac tgattgcttg aaagcaaaac ctctgtgcca
    12121 actgataatg tgctgaaatc gattattgaa tctaaccttt ctccaggtgc cagtaatacc
    12181 tgcttaatac cacctccaag ggatttcaaa aggccaccat catttccaat gatattgaag
    12241 tcaagttcag tgccatcagt ggtagaaata gagagattat atatacgagc agttgaacca
    12301 ttgataattc gcaggcgatg gaaacgtgaa cttacatttt tgaatacgcc aatgcgacca
    12361 ttcaccaaga tagtgccgcc aaaatacccg gtgaggtatt cggctttatt tggtccgtat
    12421 aacatttggt tatcttcatt gaaacgcttg tcttgcaaag agatggttgt gctgcgtaag
    12481 tttccatttg gcagattcaa tgcatcttcc tcagaatcac ttacaatgaa caaaccgcca
    12541 agtccaagac ctaattgatc gccgccaagc ttttgatatt gtgcatgata ccaatacata
    12601 cctgcacgat tgttaaccgt gaagctgtaa gatttcgaag caccggaagc aacgccattt
    12661 tcaacgccgt cttcctgaga tgcatccaag gtcaaaccat gaaaatgtaa gttagtgccg
    12721 aagctgctta agttagattc taatgtcaat gaaatattgt ctccggtgtt tgcagtgagt
    12781 gtaggtcccc aaactgattc agaatatcca tagcagaagg tccccgatat acctgcgaaa
    12841 gtatagaagt tacgatttgc ggtaatagtt gaaccattgg caacttgtgt atccaatgtg
    12901 ttgaacgggc ttacgaacgg cggggtagga actgtagtac cgccaactgt gccgaacaat
    12961 acatcatcca aaatgtcctg gtttccatct gaacagctgg caagaaatgc agctggtgtt
    13021 acggcgatgg ctgtggtgcc ggcaagtgtg ttgcgtaaaa atgatcttct gttcattttt
    13081 tgttttttaa tgtttgctca aatgttgtcg tgtgttttga tcttcagagt gcgcgattcg
    13141 gaaactgacc cgtacgctat cctatcaatc agggttctcg gggctaagat aatagtttcc
    13201 atgaaagtta aaagttttat atgttgattt ttttaatcac gccattccca actggggatc
    13261 attcccctta ttcccatgtc cacaaccgat tcagtagccc ccgcatcaag cacatcaata
    13321 tcccatttcc cgtctgtatc ggaatctatc cactcgaaac tattgttagt ggaaaacgaa
    13381 aaagtgatgt ataaatcctg cacctcatcg ccggttatta ccaacggttc ggcgaacata
    13441 cctgttacta cgcaagaccc ggcaggaata ggggaggttg cattgatagg atttacaaca
    13501 gtggtagccc cctcaggagc ctgaccggta tatatcgcgt tgtaggcact taggtcgccg
    13561 gaaaataaag tttcaaatcc ccagaaacct tgttctttat tcgcgtttac tgaagtagta
    13621 aggttttgta tatgcaggtc attgatgtaa gtgttgtatc ccagaaaaga cgctaataca
    13681 ccgtcctgat caagcacatc accataaatt gggacattat gcaggtcata agaaattccg
    13741 aattgctgat atgcaacaga tgcacgaagg tatgtatacg ttcccggcgc taatcgctcc
    13801 aatttgattt gcaaaaaatc actgccttct gatgctaaaa tggatgaatc aaaaagtatt
    13861 gcttgtgcgc ctcccgccag tgtttccggc gctttataca acaccgctcc atcgcctaac
    13921 tgcacgttgg aaccggttgt taattcaatg taatgtatac tcatcaactg catctccggc
    13981 gattgcgagc catgtcctgc aggcacttcg gatggttcac ctaaattgtc aagacgtgtc
    14041 tgtgtagaat caaatgcaaa tgaaaaatga acatcagcga atatggtaac atctttatta
    14101 caagctgaaa tccatggcaa tactgctata accggtatca gaattttttt tgagggcatt
    14161 taaatcaatt gacgtgtatt aaaactgaat ggctgcattt gttgaacaaa taaatgaaaa
    14221 atcctgctta accgactttt gctgtcggct aaaagaaatg gatcttataa tttggttttc
    14281 atcatacgta atagaatcgg aatgagaaat acatcaggcc atattgccgt aaatagcgta
    14341 acacccgtca gcagaccaat attaaatgtg acagatgtag atgaaagcat gagtatcata
    14401 aaaccgaaaa acaatattgc cgacatggtg aaaacagctt ttccggtttc ctgaaaggta
    14461 ttttgtaagg caatttcaag cgggtagccc ttttgccgtt cgattcgaaa cttcgacaga
    14521 aaatgtatcg tatcatccac cgcaatgccg tatgaaattc cgaaaatgat agaagtgggc
    14581 gcatctaatt ctatacctaa taaacccatg atgcccgcac atacgatcaa cggtaaaata
    14641 tttggtataa tactgatgat caccattcgc caatcgcgaa ataaatacgc catcacaata
    14701 ccaatacata aaaatgcaag caagaccccg ctaataatat tttttctcaa atattgtgtg
    14761 tttttatcga aaataacacc ggtgcctgtt atcgtaaatt taaccgctga agtatctgtt
    14821 ttttcttcta tgaatttctt taaatcggtt tgaaacatgc gaatgctatc ggtgccgaca
    14881 tcactcaaaa aaccactaat tcttcctgct tttttatcgc tcgagataag tgcatgcatt
    14941 tcgcgtgttg cgtatttgtc tatcaacttt tgataaccat acaatatgct atcattttcg
    15001 ggtaaacgat aataagaagc aatgtcgcca ttatatgcac gatgcatgct cttgtatggc
    15061 attgtcatcc cctgcaatcc atttacaaga tcaaatgatg atgcatatgt ttcaactttt
    15121 tctacgtccc ttattattgc cggttcagta atcaaatgct tgcctttcgc ttctgctgca
    15181 atttcaagcg gacggaagcc gttaaagtgc tcttcaaaaa aatgaaaatc ggttgtaacc
    15241 tgcgcccctt ttggcattcc ggcctccagg tgaatatttg tgctaatctg agtaacgcca
    15301 taaataaata tcaacattgc ggcactcata atgattaaaa tgcgacgctt tcttcgaatt
    15361 cctaaggtat aaatagcatt aataaaagca gtccattttt tgtcctcacc atcagttttc
    15421 gcatcgagtt gatcaaggcg aaacaaggta agcaccggac tgataaaaaa taaaacaact
    15481 gcatatacaa ccaaaacacc caacgcagaa tataatccaa aatcttttat tggtggaaca
    15541 ttggaagtga gcatggttaa aaatccaatt gccgaagtaa cggcagttaa ccatgtggca
    15601 aaacctatgt ctgacaaagt gcgaaataat gcaacttttc tattgttgtt ttttcgtaat
    15661 tcagtgatgt attttcctat aaagtgaact gcgtcggaaa taccaatgat gatgatgaca
    15721 atagggtaga gtgtactcat cacattttgt tcgattctca atccacctaa aattcctgta
    15781 aataaaataa gtgaaattgc aacagtgaga acagataaga ctacacacca tatttttctg
    15841 aacatgagga aggtaactat tgtaaccaac acaatgctaa gcatggcata aattaaaaat
    15901 tcctttttct ggatttcaac cagctcgacc tcgaaattgg ctttccctaa aagatgataa
    15961 tccttaattc cggcatcatg catcgcagct ctgacatctg ctacaaatga ctttgccttt
    16021 tgttgactaa gtgagtcatt tgttttgatg agaataacaa ccgtagaaaa atcttcagaa
    16081 actagtttgc caactacacg aacatcggca gccagtcgca agctgtctga ttcaaatcga
    16141 ctgctatcgt ccggatgaac tgccggatag tcgatgaacc cgaaaggtga tttcacaaaa
    16201 tatctgaagt ttgtcacaga ataggccctt tcggcaccct caattctgcc aaccgcattt
    16261 gtagccgaat ccattgaggt caggaacttg gcatcaaaga gcccattctc gggctgcatt
    16321 cccaccagca aaaagttgtc gtccggttca aattgctgtc ggaaagcatc ataaaaggcc
    16381 agatccgggt cattttccgg aaaaaaatct tcgtaatcgt aggaaaaccg caattgcagc
    16441 gtaaaaaaca ctgccagaag caccaacagg ctgaaaaccg caatcacata taccggtttc
    16501 aaaccggcaa cccatttcca taatttactg ttcgatacca cctcagcgca aatttacgat
    16561 ggagaatccg gattggtttg taagaccttg tgtctcagaa aaatgtcagg aaaaacctat
    16621 ttgttcataa ctaggtcaca aaagtttgtt tttacatgct aaaactgtta cctttagtcg
    16681 tgaataggtc tgatttctaa ccattaaata aaccgaaaca tgcttatgaa gcaattgtta
    16741 caaaagctaa cattctctct cctccttgtt tcagctgtca cttttatggc ttctgcccaa
    16801 acaccggaca gaggtaatgc aaccattgat gcgaacatgg taatccattt ggatgaaacc
    16861 caaccgctgg ttgccgatta ttcattttcc tttagtgcga tagctttcaa gaatgaggcc
    16921 gctgctactc gatttttcgg gctttgccgc gacaatattg tgtcctacaa ccttgactgg
    16981 gctgcaaaaa cagcaacagt gcacattgca ttggaataca tggaaccaag gggctgggac
    17041 attgtgaaat acaatgacta tttcagtaag ctcgccgaac gctatcgtac aacccttagc
    17101 gtggtgaatg aataatattt tttacaactg atcaaatttt ttattacatg caaagcagaa
    17161 taattgctgt tgtcgcaatg gtggtatttt cactcttcac catcaatagc gcaaccgctc
    17221 aaatcgttgg tgctaacgta tttttgaaag ggaattatgt ggaagtaggt atcaatactt
    17281 gcggagctta cggaacacct tctgccccgc ctgcaggcta tcatccaaca gaaggcggac
    17341 ttggattcgt tgccgattgg gaatctgacg gatggggttc aggaactccg gtatattgtg
    17401 gcgattactt cgtaccgggt tctccggttg aaggatggca gcttcaaatt ggacctacct
    17461 ggtggacgaa taccgaccaa agttgtggcg gttctgatat tcccggtgat gtttcagatt
    17521 atacatatgc aggcggtgtt tatacaggtg tttggaccgg taacctgact accggaacta
    17581 ccgatctgga tattactcag ataactacac tccctgagga tgcattgtat tttgttaccc
    17641 gaatcctatt gtgtaataac ggcgcagacc ctttgaataa tgtttattac aaaagaaatg
    17701 ttgacccgga taatgatcaa ccttggagtg gtgattttac taccgataac gttatcgttt
    17761 atcaaccacc aaccgacgaa gaggctttgg tgacttcaga aggccttact tatggttgtt
    17821 tcctcggaat tggtgcacgc gatgaaaatg cccgtgtatc ttacggaaac tttggaaccg
    17881 acgacggctc tccaaaagca gtttgggatg caaccggcgg atatgttgga acaggatcag
    17941 aagttggcga tattgctaac tcaatttcgt tttacattcc tgttatcaat cccggcgaat
    18001 gtaaatgcgt ggcctttgct tatatcctca atgttgatga cctcgaggca gcattggaag
    18061 caacagtaac ttacaacctt acggcagatg atatcaccgt tgcatcaggc gacacagttc
    18121 gcacttgtaa tccgggagat tcagttgaac tgagtatttt gggtgctgat gattatttct
    18181 ggacatggac accgacaaca ggtcttagta ccgatacagg tatctctgta atagctactc
    18241 ctgatgttac aactacctat accattaccg gtgttggcgg attctgcggt gatgcaacat
    18301 tgcaggtaac agtagaaatt gatacgcatg aatttgcaga tgccggtgat gatgtagaaa
    18361 tatgcctggg cgatgtcacc acccttacag gcgacggcgg tccatacgag aatatttatg
    18421 cttggacgcc tgccgctgat cttggctgtg ctgattgtgc tacaactaca gcaacaccta
    18481 caaccgacgg tgtatttact tacactttaa caacctacga catttataac tgccctgcta
    18541 ctgacgaagt agaagtaaca gttcaccctc tccctgatgt aaacgcaggt cctgataagg
    18601 atctttgccc tgaaggtgca gttgacctgg aagctacagg tgcaattgaa tatacctgga
    18661 gtccgtctga cggattaagc tgcgatgatt gcccggatcc gacttgtact gttgatgaaa
    18721 atactactta taccgttaca ggaacagatg caaatggttg cgtggctacg gatgtaatca
    18781 acgtaaacgt attccctaat ttggaaggta tcatgactgt agatcctaca gaaataacca
    18841 ttgatgtata tcaaaatgaa acggctcaat tgaatgctta tggtgccgaa atctatcaat
    18901 ggtctcctcc aatgggattg aataatgtca atatctctaa cccgattgcg caacctgccg
    18961 atactacagt ttatattgta acaggagttg atgccaatgg atgtaccgat gtagataccg
    19021 taactgtaaa cgtaatcggt tctctggaaa taggcgttcc tacagcattc tcaccaaacg
    19081 gagatggcac caacgacttc tggttcccaa tgatatccgg ttccggttgt gttgaaagct
    19141 ggacagtgtt taatcgttat ggtgaagttg ttttcgaggg caatggattc tgtccaggcg
    19201 aggttacgca aggttgggat ggcacttata atggagaatt gcaaggaatt ggtacctatg
    19261 tcgtgatcat taaagctgct acgacattgc ttgaagatcg atttgtgcag ggcaatttca
    19321 cattagtacg ttaatattga atggaacccg atattcatga acacaaacaa atattgctaa
    19381 atttataaac ggaatgatgt taagggcatc attccgttac aaaccaataa accttactaa
    19441 tgaaacactt acacagatta cttcccctcc tgatcttgct tacatgcaca caaatgcaag
    19501 ctcagatagt aggtgcaaac gcctttctga aaggtaactt tgttgaggtg ggtatcaata
    19561 cttgtggggc atacggctct aatggagctc ctcctgcagg ttatcaccca aacgttttta
    19621 gcggcctcgg atttgttgcc gattcagata tggatggctg gacaaccggt acaccggatt
    19681 attgcggcga ttactttgta ccgggttcac cggttgaggg atggcaagta caaataggtg
    19741 gtactgtata tacgaacacc gaccaatact gttggacttc agaaattccc ggtgatataa
    19801 catcgtatga ttataccgca ggaatttata ccggtatttg ggaaggcacc attgctgcag
    19861 aagatgtgac aattacacaa acaactacat tgcctgagaa tgcccgatac tttgttaccc
    19921 gtattctcat ttgtaacgat ggaccaactg accttacaga cgtatactat atgcgaaatg
    19981 ttgaccccga tcaggatcag ccattcggat ttagctttat gactaccaat agcgttattt
    20041 tccaacctcc tgttgaccct gatgcattgg ttgaatctgt tggtgatgta tatggttgct
    20101 acctaggtat gggtgcacgt gatgaaaatg cccgtgtaac ttatggttgc ttcagcacta
    20161 cagacggaac ccctgctcaa gcctggtcag gcgcttcagt ttgtgcatgg agttcaggat
    20221 attcactttc gggaacctat tattgtgatt gtgctactca ggtaactttt aaaattccat
    20281 tgatcgctgc tggtgaatgt caatgtgtcg cttttgctta cattctcgat gccgacgacc
    20341 ttgatgaagc gctagaagca acttcctcaa ttggtgttgc cgcagatggt gtagacatct
    20401 caacttctgg tattactttg atttgccccg gtgattctgt tgaactgaat attatcggcg
    20461 gtgaagacta tgactggacc tggactccaa caaccggttt gagctcaggt acaggcgata
    20521 cagtggttgc ttcaccagat atcaccacta cttatctcgc taccggtgta ggtatttgtg
    20581 gaacaataga acgcgaagtt acagttgaag tgccaccacc accaattgca aatgccggac
    20641 ctgatatgtc tttatgtcct tccgatacaa ttcacctggc aggaagtggt ggcgttcatt
    20701 accaatggac tcctcctgtt tatcttgatg acgacacgct ttcaaatcct gccgttatgg
    20761 atccgctgac ggatatgtat tatcagttag ttatttggga tgcaaatggt tgtaccgata
    20821 cagatgatgt aatggtaact ttatatgatt tacctatcgt agatgccggt gatgatcaat
    20881 atatggcaga aggcggtttt gctcaactta ccgcatcagg tgccgctact tatgtatgga
    20941 cacctgaaga tttccttact aacgcactga tctacaatcc aatcgctcaa cccgaagata
    21001 ctaccatgta ttatgttgtt ggtactgatg ctaatggttg tgaaaatatt gatagtatga
    21061 tcgtttatgt gttagaccaa acacatatcg tagtaccaaa cgcattcaca ccaaatggtg
    21121 acggattaaa tgatacttgg aaacctgttt tgataggaat tggtgaaatc acctatttct
    21181 ctgtgtataa cagatgggga gatttgctgt ttgaatcatc tgatccaaca atcggatggg
    21241 atggaaccta tcaagccgat gaacaagaaa ttggtaacta tatcgtaatg gtgaaaggtc
    21301 ttgacggatt tggtcagcca cttaccaagt cagcaatggt gttgttgatg cgataaagat
    21361 tgcatgcaaa atatttaatc ccggtattgc aaaatgccgg gatttttttt tggaaaacgt
    21421 tttgatattt gttgactgcc attaaaaggc aaataagaac tattcacaga tattgttact
    21481 tatacattgc aatatcctaa tgtttcatca gtgcagttgc tttttagctc tcctcagtaa
    21541 gtaattatca ggatagaatt gcaatccggt attaaatgca ttcttagctt tttgtttttc
    21601 tccaatatgt tcatacaata atcctaattc cagaaaccag tcggcacgtg agctcagcga
    21661 catcgcttgc ttgtaatctt cgatggcatt ttcccaatcc ttactcttcg cgaataatgt
    21721 agctctggca atataaaata cggtgtttgc aggatttaac tgaatggcct tgtcgtagta
    21781 tgataaagct tttagtaaca tttcagaatt tccgttgaac gcataattcc aagaaatcac
    21841 atttgcaagg tgataccagg tatcggaaga atccggatac attgttacca tttgaaacat
    21901 tgctcttgtt gcctgttggt aaaaagcagt tataaatgct tcatctttag ataaaacata
    21961 attattgcaa gcttcataat gattgtaatt caaactatcg agttgaacca ggctcaatct
    22021 atcattcatg gcaatgatcc gcatgttatc cgagtgaaat tgccctgtat aaacagcctg
    22081 ctgagataaa accgtaatgt cgtccggtgc ccgctcaatt gctcgctcgt aatcgattaa
    22141 tatactgtct tgcgggatgt tacctaatgc aatatgaata gaggctctgg caatataata
    22201 atatgggaaa tcaggatctt catttattaa gccatttagt gcatgaaggg cctttaacgt
    22261 atccgcccgc tctaccgcat cataataatt tgtgagcaag ggtttggtgg tgctattaat
    22321 aatctgtagc ggttgcgcat agctatgtac cacgcacagc gaaacaaaaa ttatcatcaa
    22381 taccggacgg atgcgcatta ttgcttactt gataggtcaa atctactgca attgttggtt
    22441 gtgaaagtaa ggcttaacat agattcgtta tttttatggt ctagttactc aaagtgatga
    22501 cccagatacg cctatccttg ttatgcatgt tattatcatg cctttcaaca tccatgcttc
    22561 atgcccagtc actgttaaca tgcggcactg acatcgaacg cagtaagtta attgccgacc
    22621 atccggaaat tctggataga gaagcaatat ttgatgcttt agcagcgaag aaatttgctg
    22681 aagtggcacg tatcgagggt gatgttttgg tgataccggt tgtgtttcac gttgtacatg
    22741 attacggtcc tgagaatatc agtgatgcac aaatatttga tgccattgag catatcaacg
    22801 ctgattttag cgcaacgaat accgattttg ccgctacgat acctgaattt gcaggaatag
    22861 cagcaaactg tcagatagaa tttcgcttgg ctcagcgtga attaagtggt gcttgtacaa
    22921 atggtattac gcgacatgct tctacgcgaa catatgaagg ttcagacgct gccaaacttg
    22981 gcgggtggca atcgggaaaa tatttaaaca tatgggtagt gaaagacctt acaaatggcg
    23041 ctgcagctta tgcatattat cctaccagca tcgcaggact tatgtatact gttgatggcg
    23101 ttatatgccg atatgattat gtaggtacaa tcggtgctgc cggatcctat gcggctcacg
    23161 ttttatcgca tgagatcggc cattatttga atttacaaca cgtttgggga aatagcaatg
    23221 cgcccggagt ggcttgcggt gatgatcaag tgcctgatac acctatgaca gagggatgga
    23281 ccacctgcaa catctatggc aatacctgca cggcacccat agacaatgtg caaaatcata
    23341 tggagtattc ttattgtagt accatgtttt cagaagggca aaaaagtcgg atgtatgccg
    23401 cattggaaag tgatgatgcg atgcgtaata atttacctac tgaggagaat ttattgttga
    23461 cgggaaccaa tgatggatat gtagcatcaa catgcgctcc gatagcagat ttttatactt
    23521 cacagcgata catttgtgcc ggcgaaagtg tcacctatca tgatgtaagt tataatggtg
    23581 aagtaacatc acgcgtctgg acttttgaag gaggatcgcc ggcatcatca acagatattg
    23641 cgcctgttgt tacttatgca acagagggtt ggcatcaggt aatactgaat gtgtcaaatg
    23701 gagtgggaac cgatacaaaa acctggaata actatttaca tgttgctcct gcagtttcaa
    23761 tttacgatga gaattatttt gaggatttta ataatcaaac cgaagttgaa aatgattgga
    23821 ttttatacaa taagtatgaa gacccctatg aatggaaatg gcgcggtaat aatggctatt
    23881 ataacacagg ttgtatttgg ctgaacagca gatttggtcc ggatttagaa tcagatgtgg
    23941 caatttcacc aatgtatgat ctttccagtg gattgacgga taatttattc ttcaaatatt
    24001 ctacaacttc atacggcgat aatgaagatg actacacaat gtcgttgaaa atttactatt
    24061 ctataaattg tggtgacagt tggatatata tgaataaagt aaccggcgcc gatttgatta
    24121 caagctatgg cggaagcagt aatttctttc cgcaatatcc tgatcaatgg gctgctgctg
    24181 cgtttaattt gcctgaagct tgtaaaactg accacgtgca attcaaaatt gaatttttat
    24241 acaaccctta tgtaaacaac gtatttattg atgattttaa tttcctttca ggcgtattga
    24301 atacaaccga acaacaagct tcagtggcat taaaagtata tccaaatcct gtgcatgctg
    24361 atggtagttc taccattcat tacagtttga cttcatcaaa tatggtaact atttccatca
    24421 gtgatcttgc cggtaggact attcaggtga ttgggaatgc acaagagcag gagggaagtc
    24481 attttataac tatttcacca aaagaacttg gccttccttc gggatgttat tttgtgcaga
    24541 tcaaagcagg aggtcagttg gtaagtgata agcttattgt aatttaatct tcgacaagca
    24601 attgcaataa tcgctgccgg tagtcgatca cagctcgata tttgtttaaa atatcgcctc
    24661 ctaatatggc tacaacgggt tcgagctttg cagaggtgta agcttgattc actacgctaa
    24721 gatctattac agcaaatgtg attggtttta aatgatattt cccggctttt atgccgcgta
    24781 attttagaaa tactgcattt ggaatattag ctcccagacc tgttgtaaga tgttctgttt
    24841 cgatacgtcg tgcagaagga aacatgcgat ttgcaaaatc ctggtctatc acacttttac
    24901 tcgctccggt gtccagcaaa aagcgaatag tttttcgttg aatttttccg tgaataaata
    24961 tgtgaatccc tttgggaggt atctcttcga gaataagtgg gatttgtgcg atctctatca
    25021 ttgattaaaa ataaaaaaac cgcaaagcaa ttcaagagct ctgcggtttt caaaaatatt
    25081 gattcaatta atttgcagca ttcatgcgat caagcacatc cattacttca cggactgctt
    25141 ttgcagaatc gttcaataat tgcatttctg atgcatccaa tttcaattga ataatttctt
    25201 caataccatt gcggccaagc ttacaaggca cgccgaggta gatatcttta aggccgtatt
    25261 caccattcaa ccatgcacaa acagggaata tgcgctgttc attttttatg atcgcctcca
    25321 ccatttgggc agcagctgct cccggagcat accatgctga agttccaagt aaattcacaa
    25381 tctcgccacc accaactttt gtgcgttgaa caatagcatt cagtttatcc atgtcgataa
    25441 gctctgttac aggaataccg ctaacggtag tgtaacgtgg aagcggaacc atagtgtcgc
    25501 catgaccacc caacaacaaa gcttgaatat ctttaggaga aacgtttaaa gcctcagcaa
    25561 ggaaagcgcg atagcgggca gtatccagaa taccggccat accaaaaacg cggctgctgt
    25621 caattttggc agtaagatat gcacaataag tcatcacgtc taatgggtta gaaaccacaa
    25681 tgatgatggc ctttggtgag tattttacta cttgctctgt cacagattta acaatgccgg
    25741 cgttaacact aattagatca tcgcggctca taccgggttt tcttggtaat ccggaagtta
    25801 tcactaccac atctgaatct gcagtgcgcg cgtaatcatt ggtaacaccc gtaacacgcg
    25861 tgctatagtt gtcgattggc gcctgttgcc atgaatcaag gctctttcct tcggcagtgc
    25921 cttctttgat atcaagaacg atgatttcgt tcaaaaaatc acggtgagcg aggacgtttg
    25981 ccacagtgga accaacattg cccgctccga taacagttac tttactcata gttgtgtcat
    26041 tttagctact aaaactggac ttgcggctgc aaaattaggt gttttacagc gcacgtaagg
    26101 tattgtgcac aaactttgct gcaatatttc tgttaccttt ttaactttac acatcaattc
    26161 cacatccata gttatgaaga aattactttt gattgctgca gttggcttgc tgggaggcgt
    26221 tttgcgcgct caaacagctc cttccggata ctgcggtaca ctacaaacag aatcagatct
    26281 tacctggctg cgaaattacc aggctaccta cgttccgggt gaactggaac gcggcggtac
    26341 aacctattac attcctacta aagtgcatat tgtaggaaca gatgatggca gtggctatta
    26401 caatctgagc tacctatatc agtcattttg cgaattgaac gagcactatg ccgaaacggg
    26461 attcatcttc tatatctatg gtgacctgaa ttacgttgat aacactgatt attacgaaca
    26521 cgattggttc gatgggtcga atatgatgga agattacaat gtaaacaact tactgaatgt
    26581 ttacttcgta tctgaccctg caggaaactg tggctatttc agtccttcag atgatggcgt
    26641 agctgtagct aaatcatgtg caatgccggg tggaactacc atcgcgcacg aatttggtca
    26701 ctttttctca ttgccgcata ccttttacgg ttgggagtgg ggaacaccgg acgataatga
    26761 tcaggaatgg gtaaacggtg caaactgtca ttcagctgcc gacggatttt gcgatacacc
    26821 accggattac ctcccttacc gctggaattg ccccggacca ctgcaaacgg atccacatgg
    26881 cgaaactttc tatagcgatg gaacattcta tatgtcgtat tcaaatgatg cttgtaccga
    26941 caaattttct gcagaacaac aagatgcgat gatcagtaac ctgttgggac caagaaataa
    27001 cttccttgac tatccgactc ctgcatatgc agatcttaca gaggcctcaa ccctcattac
    27061 tcctgaggat gctgccataa accagttttc taattatttg gtatttgaat ggactcccgt
    27121 taccggtgca acacagtatc acctgcaatt ggcttacaat gcaagcttta gtgccatcgc
    27181 caaagatgtt gttactacca atacatttta tgaagcaacc gacttgcaga tggataaaaa
    27241 atactattgg agagtacgcc cgcttgcacc ggctaatact tgtgcatctc cttctaacgc
    27301 aagatcattt acaacaggca ctgaattaaa cagcgttgca aatagtgaaa tatttgatca
    27361 cctggctgtt tatcctaatc ctgctttagt tggtcaaaat cttacaatgg aatatcgttc
    27421 agctgaatca ttccgcgccg acattagttt gctcgatatg acaggccgcg aattgacctc
    27481 aacaaactat caaataaatc aaggtaataa cacacttgat gttgctgttc ctgcggtatc
    27541 atcaggtatg tatatacttc gtattacagc cgaaaatcgc attgaaacca tcagtgtgat
    27601 gatacgaaat tgatatcatt tacgtaagag attattttgt tgcatgctcc tcaatcgggg
    27661 agcatgctta tttttaagac attggtggtg ttgttggaaa ccctgaatcg ctcatcgctg
    27721 cggataccaa ttaaccaaag aatatattct ccgctgcaca atacgaatgt attttccttt
    27781 tcaggaatgg cgatgcgtgc atccgttaat atatcgctga tcttcttttt tcccggtttg
    27841 gttttccctt ttttgaatag gccaagcgga tacatataat cacccttttg ccatactcgc
    27901 aatgttagcg ggaattcaat tttatctgca tcgaaatagg caatattatt tgaagcctgc
    27961 agattgatat ttggatgcat ggccattttt tcaatcttca atttaccatt tttaatatga
    28021 taacttcctg aaggattttc aataatttga atgttggcgt ttacttgcgg ataacttgta
    28081 agtaaaaaat gttttctatc tacaatcagt ctatgtgtat tagttaaaaa ctgctttcct
    28141 gatacatgtt gttgagtcgc caattcagtg atctgtgcat gcgtaaatcc aaatggcgcc
    28201 aaccatgtat gtagtagggt agaggcagca gggtgattgg ttattggctt tatgggaagt
    28261 gatatggcag tcggcgtcgt cttcaaccat ttatgcaaaa gcattacggc cctttcatcg
    28321 gcaagcagat gcgcctcacg aaaatgttgt gcattggttg ccattgtttg caaaaaagat
    28381 ggatttgttt gctgcaaaaa cggaattacc tgcttgcgga atgcattgcg caaatagaca
    28441 tcgctgttat tggtgacatc ggtgcagaat gtcagttgat gttctgaagc ataggtttca
    28501 atttgctcgc gcgttgcaaa caataagggt cttataatat ttccgttgcg cgctctgatg
    28561 ccatgcattc cctgcaatcc ggttccggaa gtaagatgca taagcatagt ttcggcatga
    28621 tcatttaaat gatgtgcggt tgcaatgaaa tcataattgt tttgaattcg aatttcttcc
    28681 agccattgat agcggatatt tcttgccgct tcctcaatag atagtttgtt tgcgcttgca
    28741 tatttttttg tatctacact ggtggtgaaa aatggaacat ttaacgaggt cgctaaattt
    28801 ttaacaaagg cctcgtcttt ttcagcatct ccatcgcgca gctgaaaatt gacatgtgct
    28861 atacctatgg tataattagc tgatgcaagc aaataagtca ttaccacact atcaacacca
    28921 ccactcaccg caagcaatat tctagcttgt tgcgtaaaac ccggcaatgt gtgcaggaat
    28981 ttatggagtt gatgtgtaga gagcttatcc attacttaat cttcatcatc cagtcccaaa
    29041 cggaaattca atgctgcatt ttgcaactca gccagtgcat gttgatctcc tagtttttgt
    29101 gcaacagcaa tgccggattg ataacattgt tcggcttgtt caggcaattt catactgatg
    29161 tataatttgg caagatggta ataggtgcca acatagtcgg gttgaatatt tacaagatgc
    29221 tcaaaatatt tacgcccttc ttccttatct ccgattttga tgtattcaag agcgagtgca
    29281 taattcagaa atgcatcacc gggattatcg tttagataac ttaataattg tggtattctt
    29341 tccatgtata ctccttgccg tcaaagatac tctaccttac agaaatacct ttcagcccat
    29401 acaattcgcg gttggtgatg atatatgatt ttgggtttta tgacctatct ttgcagtccc
    29461 aaaagttaag cctaatgaaa ttattagttt gtatcagcaa ggtccctgac actacggcaa
    29521 aagtggcctt taccgatggc aatacccgct ataatgaaaa cggggtgcaa tttatcatca
    29581 atccatatga tgaatggtat gccttggttc gcgccctcga attgaaagag acccttggtg
    29641 gaagtgtgac ggtaattaac gttggcaagg ccgataatga acaaattatt cgcaaagcgt
    29701 tggcattagg tgccgatgat gccgtacgta tcgatatgga tcctgcagat gcctggcaag
    29761 tagcttctca gattgcacaa tatgcaaaag gtggcggatt tgacatcatc ttcaccggaa
    29821 aagagacaat tgattacaac ggaggaattg ttggcggcat gttagccgaa ttactcgacc
    29881 ttccctatgt agcgcttgcc gcaaaacttg atgttgatgg cactcagctc aatattgcgc
    29941 aggaaatgga aggggtgaag caactgatca aagtgtctat gccttgtgtt gtttctgctc
    30001 agaaaggaat ggctgaagct cgaatcccga atatgcgcgg tatcctggcc tccaagtcaa
    30061 aacccgtgca ggtaattcct gcttcaggtg atgctgctaa atctcctgtg aaagtatttg
    30121 tgatgcctgc cgagaagcag ggttgtaaaa tgatctctgc cgagaatccg gaagagttgg
    30181 tgcaattact gcacaatgag gctaaagtaa tttaatttac ctatcagaaa acgacgtatg
    30241 caactcgtat tagtagaact tatcaacgga aaatgtaaaa aaacagccct ggaagcagta
    30301 gcgtttgctg ccaaaatggg tgaaaccact gccttagccc ttggaacagc ctcagcagat
    30361 accttgcagg cgctcggtgc ttacggaatc ggcaaagttt tgcatatcaa caatgaaaaa
    30421 ctaaatcagg tatattctaa agcctacaca aaggctatag cagcggccgc aacgcatgtt
    30481 ggtgctgata ccgttatttt atcacacaac tcaacaggac gtatggttgc cccacgtgta
    30541 gcagtgcgta tggaggctgc tttatttgcg ggggtgaatg aattaccggg aaatgatacc
    30601 atccgcaaag ctgtattttc aggcaaagct tttgccgatc tgcagatgtc aacgggtaaa
    30661 aaggttatat cgctaaaccc aaattcatac aaagcagttg ccggcacagg cactgcatca
    30721 gtggaatctt tttctgttga ttttgctgat tctgatttcg attatgcaat caccggtgtt
    30781 gaagatgaag caggtggtca tgtgccactg ccggaagcgg agatagtggt ttcaggcgga
    30841 cgtggattga aaggcccgga acattgggga attgttgaag atcttgcgaa ggcattgggc
    30901 gctgctactg catgctcacg cccggtagca gatatcggat ggcgtccgca tcatgaacac
    30961 gttggacaaa caggtatcac gatcaagcca acattatata ttgcagttgg tatttccgga
    31021 gcgatacagc atcttgccgg agtaaaccaa agcaaagtga tcgttgtaat caataaagat
    31081 cctgaagcgc cattctttaa agccgcagat tatggtattg ttggcgatgc attcgaagtg
    31141 ctgccacgca ttacagaggc tgtgaagaaa cttcatgccg gggcataatt atcgaattgc
    31201 ttatcattgt taactcattc agtaagttgt atcttagcct gtacgcatgc agaaagttga
    31261 acttgaaata acagggcttt cacataccat tacccagtcg cagagctatg ccgtgttatt
    31321 gagtgaggtg catggtcaac gcaagttacc gatagttatc ggtgctttcg aggctcaggc
    31381 aattgctgtt gccattgaaa aagtggtaat taacaggcct ttgtcgcacg atcttatcaa
    31441 aaacatgttt accgtttttc agatcaatct gcgtgaagtg cttattgata atttgcaaga
    31501 gggcatattt tattccaaat tgatctgtga aaaggatggg caggagttcg acattgactc
    31561 cagaacgagc gatgcgctag ctttagcagt gcgatttaat tgcccgattt atacctatga
    31621 gtttatactc gacagtgcag gtgccatcat caacgagccc gaacctgatg atgataacga
    31681 agaagatgag aatgaggagg aagatatcga tgcgttagaa gaattatccc caaccggatt
    31741 ttcatcagaa agcggttctg acctcacgaa tatgtcgctc aaagaactca atgatcgcct
    31801 tagggagttt ctggagcatg aagattacga atctgctgca cgcatccgcg acgagataga
    31861 aaaaaggaaa aaaccttaat aatgtcgttt tccaccataa tatggatttt tcttggtggt
    31921 ggatttggca ccttggtgag atatggaata ggcacacaag tttccggcct ttccaagtct
    31981 aatttcccga taggaacgtt ggctgttaac ttaatcggat gtctaatcat tggattgatc
    32041 tacggatact ttgagaaaca tcaggaaact cagagcgact ggaagctcat tttaacaatt
    32101 ggcttttgcg ggggctttac aaccttttca gctttttcga tagatgccat ccaactggtt
    32161 aagggcggaa atctgctatt ggcgatttta tatatcttta gcagtgtatt tataggcatt
    32221 ttgctttcta ttctcggttg gcaaatcgct aaaaattact gaaaaccgca acacaattat
    32281 taacttttat ggtagaaagt attttccgtg gcttacttgg tgtagccttt ttaatcgggg
    32341 tttgttacct attcagcaac aatagaaagt ctatagattg gaaactggta ggttcaggct
    32401 tgctaattca atttgtattt gcatttgtga ttattgcgct acctgatatc actgaacaac
    32461 attttggata caggtttgat tggtttgaaa gaatttttca aggaatggcg aatttctttg
    32521 tgatggttat cgggttcaca aatgcaggag caaatttcgt gctcggagac tggccatctg
    32581 taacacaagt ttctgatgga ttaaccggac aagtgtttac cgttggattc atttttgcgt
    32641 ttaaagtttt accaacagtt atttttttta gtgcactaac cagtttgctt tattatttag
    32701 gcatattaca aaaaatcgtt tttggatttg catgggtgat gagtaaaaca atgcgtctga
    32761 gcggcgcaga gagtttaaat gctgcaggta acatattcct tggacaaacg gaagctccct
    32821 tgttgatcag gccatatctt gagaaaatga caaaatctga agtgttatgt gttatggttg
    32881 gcggcatggc tactattgca ggtggagtga tggctgcata tatcggtttt ttgggtggcg
    32941 atgatataga aaagcagaaa atgtttgcca tgcatcttct cagcgcatca ataatgagtg
    33001 cacctgctgc cattgttgct gcaaaaatgc tgtttcctca aaaggaaaat gttgatacag
    33061 aacttaaagt aaataaagaa agaattggta gtaatgttat tgaatctatt gccaatggca
    33121 ccaccgatgg actaaaactt gcagttaatg ttggtgctat gcttttggcg ttcactgctt
    33181 ttatggctat gtttaatgcc atatttcttg gcataggaaa tatgctttca ataaacgaca
    33241 atatcgctgc ggctactaat aatacatatt cagggctctc tttggattac atcttcggta
    33301 tcattatggc acctgtggct tggcttcttg gagtacccag ttctgacatg ctaatggttg
    33361 gacaattact tggtgaaaaa actattatta atgaattcgt cgcttattcc agtttgggga
    33421 atatgaaatc gatgttggat gaaaagtcaa tcattatagc aacctatgca ctttgcggat
    33481 ttagcaattt tgcttcaatt ggcattcaga ttggtggaat tggtgccctt gccccgaacc
    33541 aaaaaacaac actttcacaa ttaggtctcc gcgccttact cggaggcaca gtagcatgct
    33601 tttttacagc tgcaatagcc ggtatgatca tttaataata ttacgatgca ggaatttaca
    33661 caatgggcaa tcttcatggg ctttatcgcc ctgatggctg ccatattata cgcctatgct
    33721 tcaggttggg taaatagtcc acgtaccaga agagaaagct ttaaagaatg gcagaaacta
    33781 aatggtggag tagtcagaat gatgagcctt ctggttattt tagtttgcgt aatcgtttgt
    33841 atttataaat acaatgatat caaagcagcc attgatgcac cacctacttt gcaaactgaa
    33901 tgatgatcat ttgaacagct gttcagtata tggatgattt gaaaaataca cgtacatacg
    33961 cgtagcctct ttcattcttc ctttactact tgcatgtatg cctccttttc cggcatcatc
    34021 gcagttccag gtatagcctt gtttatctcc tttgctgcca ttagtccaca acgttggccc
    34081 ccagtcaata aaaggaattt cttctttagt aaatcctgcg tcattattca tttgatgctc
    34141 aatcacccat tttactgtcc aactatgaaa atatgctgtt ggttctgcta attcttttgg
    34201 ccccattcca tttacgccaa tgtatccggc gaaaggcctg tcgcttaaaa aaactattct
    34261 tgcgtttggg tattgcaatt taatttgctt caaaaccaaa gcatatttat ctacctgttg
    34321 caaactctgt tgagggaagg gtaggctgta tgttaaaata tcacctgtgc tgatccaaat
    34381 caattgaatc tgagcagccg taattgtatt tgagtgaagg gcagaatcta caccatccca
    34441 ataagaagac aaactgtcgg tcattgaatt aatatctctg cctccctgtg caccgattac
    34501 aacctgcaat tttgtatccg gcgattttat tgctaccaca tcgcgaaaga atctccctgt
    34561 catagcagca gtgctatatc ccaaaattaa aaaaccgatt ttacctgaaa ttgaatcttc
    34621 ctttccttca gcattgagcg gtttaatgtg cgatgcaaca ctaagtcctt cctgaaaatg
    34681 ttgcatcgga attgcattac ttccatttgg atataatccg ccttgatatc cttgaaatgt
    34741 tgacgacccc aaatctgcta ttggaatgta ttcagtattt ctatcatcgc aaggttgcgc
    34801 atagataagg aaaaagctaa gttgtaatat gaagaatgtg aatgctcgca tgatcaaaat
    34861 ccaaatccga taaatagtcg tgcaggagga ataaaatcat catgcatagg tgttatggca
    34921 aggtctacgc gaataatttt aaagagatgt tctacgccaa aagtaaattc tgtatagaac
    34981 ggcatggtgt ctgatgtcaa cacatgtgca ctgaatacag gttccatttt taattgctta
    35041 aagagtggca atttcctaaa caaaaatcct tcggtatgcc agctgacatg gcctgtaaac
    35101 atataatctg aagcagttgt ataataatag ggcaacaaat aaaaggtttc attctgtaag
    35161 cggcttacat tggtttcatt accaatagca tgtatcttat caacgattaa ttgctcgtaa
    35221 tcggcaaact gaagtgttgc tgttccgata tattccaggt taccgacaag tgacagaggt
    35281 aaccgatcaa aaacactaag gtgtatttta ttaaattgat ctaattcatc atttaaggtg
    35341 acattggctg aacgcagatc cagttcaata acaggatact ttggctcatt aataaatttt
    35401 tcatcaggat gcagaatgta ggtttgcttt atcacatatt taatgtggag atgcaacatt
    35461 aaaatatcag acattggccc ccattcaaat acggtagcat tgtgtatgac gaagtcatta
    35521 ttattggaaa aatatttcga ctgatcatct atccaaggtt ctgtattaat ggcatcatat
    35581 tggcggtaac gtttgccata atatgctcct atgccaaagt agaagccatt atacatttcc
    35641 cgatcggcat caaactgaat aaaatcttct gcatataact tcatgctgtt ctgttccagc
    35701 aataaggtgt acagcgaatt taccaatggc gatataccca tgggattaaa ttgttgcatt
    35761 ctgctacctg ccaatagtga taatttttcc tgcgacacag gatcaagtaa gtaacgtgac
    35821 tgcatcgaaa tcagcgctcc gtttgttgct gtattccaac gcagcgtggt gccaatttcg
    35881 aagcgtctgt tatcttcata ccagtgggtg taggtaagac gtggttgtat cacataattt
    35941 tcaacagtat taaattgcaa caggtcgaaa atattatttg tgctaatcat tgactttgat
    36001 ttgctgtcgc gccaattata accggttatt aatacagcag gtgaaaattt attaaagata
    36061 tgatccattg aatcctgata agccggcgat tctttcaaaa ttgccaatga atccttttca
    36121 tgataatcca attgctcttc cttcgtcagt tgcataggtc ggatcccagc ccaataagtg
    36181 ctgtcttgct tatttgctcc ggcattgatc ctgattactt caccgtcaaa aaaatgctta
    36241 tcaaagattg ggttgatggt gtaattcttg taatacgcat gaaaatagcc ttcaccctga
    36301 atgccaaaca tgccgtaatt gaaactgaac ttattcgaaa gcaacatcaa atgttcgcga
    36361 tctgcataat agtattcctg tgtcacatta aatgtatcca ggaattcaat accattttgt
    36421 ttggttagtg tgaggtccaa tcctgtgatg cgataatcgt cgttggtgat ataaattacc
    36481 cccgtgtagg caggatcacc ggagcgctta ggcgtaactt gtattttgaa aatttcgcga
    36541 ttgttttcaa acgtgttgcc gagtaagtga taggtgtaga aaaacaatgc attgtcggca
    36601 ataggagaaa caaacccacg ttgcgaaaac ccttgaagtg tttccagatt ttgataaaaa
    36661 ttcatttgca tgccggcagc atcattccag ctgaattgat ttttgtcgcc acttacaacc
    36721 gaagctttca tgatctcctt ggtttgatcg ggatatttat aattgaagct tgaaaccgat
    36781 tccgacaagt aaataatacc tgtattattc gaatcgacat caaaaaccgt atttaacttg
    36841 atgcccatga tttgatcagg cgcttcattc aatttttgaa gtcctttcat atatacatca
    36901 caactgtatg cattcacttc tttcaagtga tctgcacgtc gttctatggc ctgttttatt
    36961 acagcgtatg caggatcttc atgatcagga ccaattacta tcgtttgcaa ttcggtcatt
    37021 tcttcctgta aaacaacatc aaacattagt ggcttatctg aaatggtgat aggtatttcc
    37081 ttgcggctgt atcctataaa ttgaaatact atcacgtatt ctccatttgg agcagtaagc
    37141 tgataaaatc cttccgcatt tgcaggaaca gcataagtag ttccactgag ataaacatta
    37201 caaaagggta gtggagcgcc ggtggtatcg gttatatgcc cggagatggt acctgctgat
    37261 aagcgggtaa taagaataat gagcagacat acataaatca aacacttacg catacaaaag
    37321 ggaacacaaa tataagcagg gagttcaggt attacctgaa aacataacta acgccaccgt
    37381 gcaatccgaa gacaaaattg cgcttggcgt agtcatttaa aggttctata tgcgtgctga
    37441 tatcattaga gaaggtaccg cgggcgcctg ctgatatcac gatattatcc tgaacaaaca
    37501 catcatattc caatcctaaa accacagata catcagtttc tttaaacaat tccatggcag
    37561 attcttcata gcgtttgtct tgtggcatac gtgcttcgcg taacataccg tattgcacac
    37621 cggcagtata attcaacact acaggtttat tggtaatatc tgagatatgt ccaattttca
    37681 ccttagctac tactggaata aggtcatagt ttaaactgag tgatttttca acgatatcgt
    37741 caccttcaga catcacataa ttctgtcctt gcagtgcatt ataaacatat tcagcttcga
    37801 taccgaaact attgttgata ttataaccaa ccctaagacc tttggtagtt ccaaatttca
    37861 gcgaaggttg aattggtctc tcgcctttaa atacattgcc ggattccaat aatgaagttt
    37921 gattatagct acctgtagcg ccgatatata atccgcgcat atcgtaatcg cggtattttg
    37981 ctttataaga aggcgcttca aaaaaatcag cttcaggatc atcatcacca aaataattgt
    38041 atgccatcac atcgatgggt tcatcattat tgtagaagcg atcgaataca tcttgacttg
    38101 cggcagctaa ggtttcattt tccggctctg cagggagata aataaatgca tcttttggat
    38161 gagtttttat cgacaagtcg gcagtagtaa tttgctgaac gggattctca aaggaaacgg
    38221 attcgcttgg agttcttctc agcgaaatat tatcagcaac cacttgagtt atttcaggga
    38281 tcaatgcggt aaacgaaggc attgcagtat gtgaaatagg ggccgcatag ctattgccgg
    38341 tattattgtc gaaagcaaat atggccggag gggtatgtct gttgcccggt acgctgatca
    38401 atgctacgga caacaatact aagaacattg ctgcatatcg ataccattga gcacgagagg
    38461 atttagcggg catcccatta tccaattcat ctgcaatgcg gttccaaaga tccgcagatg
    38521 gctgagctga atagtgcttt aagccgtttt taaaaattct atcgatatgg tggttgttat
    38581 tctccaattg ctttgcgttt gagacctgta cgctgttcta ccatttccat gagtatatag
    38641 cgtgcacgtg ccaactgact cttagacgtt ccttcactga tgtttagttg ctccgaaatc
    38701 tctttatgtg aaaatccttc tattgcataa aggctgaaaa ctgttttata tccgggcgac
    38761 agggtgtcta tcatttgcat taaatctgct gcagccagct tgtcgagggc attttcatcg
    38821 aaaaattcaa catcagtatg ctccacatcc atgatagggt agagtgaatt tttgcgacgg
    38881 tgaacctcaa ttgccgtatt gaccatgatt ttgcgcagcc aaccttcgaa acttccttcg
    38941 aaacgaaaac gttccagatt gcggaaggct ttaataaatc cctcctgcaa aatgtcttcc
    39001 gctgcctgat aatcaggcgc ataacgaaga caaacaccga acattttgga agaatggcga
    39061 tcatacagat gcttctgtga tacgcgatct ccctgcaaac attgtcggat tagttgctct
    39121 tcaggaatgt taatgggtat tcgaagtcgc ttcatatttg gtcaatttgg cgcagtaatt
    39181 caggcgtaag ttattaaaaa ttcgttattt ccaaaaattt tgtgttaaaa atgctgtcat
    39241 ggcagcaaat gcttttcctc tgtgactgat gggcgctttt tcctctgtct tcatctccgc
    39301 aaacgtagtg ctgtagccat ccgggataaa aaccgggtcg taaccaaaac ccatatttcc
    39361 ggtgggaatt tcggctattt tgcctgtcac aattccggta aattggtgca cgttttttcc
    39421 gataatcagt gtaaaaactg tcttgaattg tgcagccctg ttgccttcgt ttcttaaatt
    39481 catcaaaagc aggcggatat tggcttccgg gtcgcgggaa cctgaataat gcgcagtgtc
    39541 tactcccggc tctccatcca acgcagctac ctcgagtccg gtatcctccg aaaaacaatc
    39601 aacaccatac ttttcgtgca catacatggc cttttgcctg gagttccctt caattgtccc
    39661 ggttgtttcc ggcaattcct ccaaacaacc aatgtcgcga agtcctttaa ttgcaaatcc
    39721 ggaaggcatc atgagttgca cttcctttac tttgttttca tttgccgttg caaaaattaa
    39781 gtcgctcatt ttaatccgaa cagggtctgt aatcgcgccc acaactgctg tttttgttcc
    39841 ttggtaatac cttcaatatt atctgatata agtatatcta ttccattgaa attgaatatg
    39901 ttataatact gactatcgac gaataaatgt atttcatccg gcgtaatccc cagtaaaatc
    39961 agctttttga catagcctgc tgaggctaat ctgttaaaat gcaaattacc ttgcccgagg
    40021 aagggtagcg ctactgcgtt ttcttttgta agtccgcatg cggctatcat tttatcaagc
    40081 gctatcattg catcaggact aagccggttc ccggaactcg gtttgaagag gaataaacta
    40141 tgcacctcat tcgacaaatc caggccttca tcattggctt ttgtgggtga tattgttagc
    40201 tttgtggcat cagagaccag aaatacaggc tcgtcgacca ttgctcctcc gaattctagc
    40261 atacttgggc gattgatttt gtaaaattaa ggcctttaac ctattgttta accaagtttt
    40321 caacattatg gacactgcta tcaatactac ttttccgatc gagattcata agtcgcccgt
    40381 atcacatatc agcactgtag atttcgatca gcttcaattt ggcaaggtat ttaccgacca
    40441 tatgtttgaa gcggattata tagatggaaa atggacaaat tgcactattc gtccgctttc
    40501 tacgctccag ttacatcctg caacatccgc tttgcactac ggacaagcag tgtttgaagg
    40561 aatgaaagct ttcaagacaa cagaaggaaa ggttatgtta ttcaggcctg acgccaattt
    40621 ccgcaggttg aatgaatctt gtgtgcgtat ggcgattccg gaattaccgg aggaattatt
    40681 tatggatgcc ttgttccaat tggtgcgtat tgatgcagat tggatcccaa ataccgatga
    40741 aggttccatg tatatccgtc cgtttgtgtt tgcaacggat gattttgtag ggataaaacc
    40801 ttcagataca ttcaaattca tcattttctg ctgtcctgta gcgcgatatt attcaaaacc
    40861 ggtgcgcgtt tacatgcagg agcaatttat acgtgccttc ccgggtggaa ctggtgcagt
    40921 aaaggctgca ggcaattatg gcgctacgat gatgccttta atggaagcca gaaaacgcgg
    40981 attcgaccag atgctttgga ttgatggcgt aacccacaac agattgcagg aaataggcac
    41041 tatgaacgtg tttgttcaga tcgacgatac cattattacc ataccaacta ccgaaggaac
    41101 catcctgaaa ggtgttacac gcgattcttg tattgccttg cttcgcgatg gcggttacaa
    41161 ggttgaagaa agagatgtaa ctatccagga attgatagac gctcatcgca gtggtcgctt
    41221 gcacgatgcc ttcggaacag gaactgcagc tacagtagcg ccaattggtg tgatagggta
    41281 taaggatgaa gaatacgaac tccctccggt tgaaagccgt aaagcttctg cctggttaaa
    41341 gcatgagctc tgggcaatac gcaaaggaat tgctacagat cgctttgatt ggatgtggga
    41401 agtgtaaatt tctgcgaatc caattcaaca ccgtacctga ttttcctgaa aggtgtcgga
    41461 aagtgtgcta gtcgcctacc ttggtaatca tgaattccac cctctgattg gcttgtcgac
    41521 cctcaggtgt cgtattgtcg gcaacaggca tggattttcc gtatcctttg gatgtcaaac
    41581 gctctggaga tattcctttt gataccaggt atttgactac cgattccgct ctttttgtgc
    41641 tcaatgattt gcaataagta tcatcgcaca taccgtttgt gtgacctctg atctctactg
    41701 aaatgccggg attgttaatc aggagtcgtg caatgcgttc gagttcaatg aatgaagtat
    41761 cttcaagaac ggcactattt accttgaaca ggatattcgg tattttgata actatacctt
    41821 cctgcattgc cacaagctca aaatcctgaa tagcctgctt atccgctaag agcgatttta
    41881 aatcaataat ggtgtctgct gttaaatagc cctttgcaga cactaataca tgataatcgg
    41941 tagctccggg cataaccaag gcatagttcc catcttcccc ggaggtcccg gtaccggttc
    42001 tcactccggt cgactcataa tttatcattg ccttcaacgg ctgccctgta atgcgatcac
    42061 tgatgttccc tttcagccat aaaatgttgt ccggctgcaa ctcaggaggc aattgtatgc
    42121 gataaatgtc gagattggtg atggcatttt tactgctgct gaaatacccg taatcgccat
    42181 cagcatcaat cgagaaatat acgtcccatt caggtgtatt tacttcagga ccgaggttta
    42241 cagggcttgt ccaacgttgc cagctatcat ctaatcgttt tgcaacaaaa atatcctggt
    42301 tgccatatcc cggaaaacca ttagaagaga agtaaagagt cgactcatca gctgccagaa
    42361 aaggtgtcat ttcactacct gccgtattga tgtcgacgcc gagcggttta ggcacaccaa
    42421 atgagccgtc tgcctgccta aaactcacat acaagtcgag caaacccatt ccatcagcct
    42481 tttcaatcga catgagtaaa atatttccgc ttgccgacat gctgaactcg ttatattggt
    42541 tcaggttttc aaatcctgtg attacaattg gttcgggtat agtccaagtg tcggaacttc
    42601 gtttggttat actaatgccg gtatttgtta cttcagcacc ataggtgttt cccaccac
//
LOCUS       DAOQOU010000007        39300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_412_length_39300_cov_6.636616, whole genome shotgun sequence.
ACCESSION   DAOQOU010000007 DAOQOU010000000
VERSION     DAOQOU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 39300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39300
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_39300_cov_6.636616"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PLM90_01200"
     CDS             <1..1096
                     /locus_tag="PLM90_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HMZ88009.1"
                     /translation="HDVQGNLNFQPYGKPGEAINSVSRGGLNVMMIDAAEKAGVTYLF
                     EQRIMEIDPSGKKAMMEDMSTGRHYTLEPDLIIGADGAYSLVRYAMLKTDRFDYSQAY
                     EAHGYKELTIPAGPNGSFLIDRDALHIWPRKQFMLIALPNIDGSFTCTLFFSLEGKES
                     FEAIKTHADLDAFFDTYFPDVKSLIPDLHHDFFANPTGLLMIVKCFPWSHGNTMLIGD
                     AAHAIIPFYGQGMNCGFEDCTVLDTLIEEFNHDWSKIMPAYERLRKPNSDAIADLAKL
                     NFIEMRDLVADADFQFKKRIAAKVSALHPDRFIPVYSMVSFSHLPYKDALSEYHRQDA
                     VLTEILGWPEIENRWETDYLPQIAERLFNK"
     gene            complement(1284..1646)
                     /locus_tag="PLM90_01205"
     CDS             complement(1284..1646)
                     /locus_tag="PLM90_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88010.1"
                     /translation="MKIKFLLFVLAGGFVLASCSDKEAEQKIADLEKQMHVGDSACAA
                     EKTALMDSIAALHMEMEEWMKMDAMKSSTSSKSSTSTKSTTTVTPAPDKKVDVNVKGG
                     DTKKTIDIKKKGGATGGN"
     gene            1749..2450
                     /locus_tag="PLM90_01210"
     CDS             1749..2450
                     /locus_tag="PLM90_01210"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HMZ88011.1"
                     /translation="MKTKISVLSLAVIAAGGMFITSCGTEDLSVPVIVLEGDSPFIVE
                     LGDTYTDPGFTATDDEDGDITASVTVDDSDVDTEEIGEYEVTYTVTDGAGNVGTETRI
                     VRVVMGKADYMGTFQVHEICDMDGDGVYGEPGVDYEINDYTVTVTSGGDDNEVLFQNF
                     GAYGATVIVPVYFSGDLNEILTVDDYNLPGTTIYFNADGEITTGTTSDIEFDLDYNAQ
                     DGATIIPCQATFVKL"
     gene            2551..3180
                     /locus_tag="PLM90_01215"
     CDS             2551..3180
                     /locus_tag="PLM90_01215"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HMZ88012.1"
                     /translation="MRKLLPISIVIIFFTSACTRDDSTAPLILIQPDAHAPHYRTIPF
                     VDPGCEVADDYSCADAIDLQISNNVDVNKYGTYNIMYTATDEAGNINEYNRPVDIILP
                     ISDYYDFAYSAYDTCTSGNYFYTGLAQDCDCPDDKVIFGNISNFGPNATFPLPINGQY
                     NHMITLDTTKLAVTFFGTGVMSPAADTIFWEYVIMDSISTDACRSVWIK"
     gene            3212..4870
                     /locus_tag="PLM90_01220"
     CDS             3212..4870
                     /locus_tag="PLM90_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ88013.1"
                     /translation="MTTLNTIFSRIFLGILITVSHSVFAADPGRINGQIIVQFYSNVE
                     PERVIIDVELASKTDVALLKPLSASLNIWLIAFDENVYNAEFVKDAFFKNPDVALVQF
                     NHTFSERSIPDDPFFYQQWGLLNDGAGGIADADIDADLAWDITTGGVTAKGDTIVVAL
                     ISEGQFYAHEDLDYFINRNEIPDNDIDDDANGYIDDYHGWNGTTQTDSVPERLHGTHV
                     AGIAGAIGNNGLGISGVSQKLKILPVYNLTVEADAVASYAYARDMRKIYNETNGEKGA
                     YIVATNSSFGVDFGNPDDYPIWCAMFDSLGVVGVLSVASTANTSVNVDNVYDIPTACP
                     SEYLITVTMTDKADNLFSAAYGAKSIDLGAPGNLIYSTIIDNSYGILSGTSMSAPHVT
                     GAIAMMYAAACPEFFDALEDNNELMLLKLKQYLLEGVDVVPDLEGVTVSNGRLNVFRA
                     IENLTVSGFCATGFAENALGVAYPNPAQELLFINGATIIDQEVQVQVVNTLGQVVYQR
                     TGDRSPFIFSGIPVGDLPSGYYAVSVYNPDSDLLFGSGVIIQHQ"
     gene            4887..6587
                     /gene="recJ"
                     /locus_tag="PLM90_01225"
     CDS             4887..6587
                     /gene="recJ"
                     /locus_tag="PLM90_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HMZ88014.1"
                     /translation="MDKRWVIKVTEEEKVQSLQQELKIHPVICNLLVQRGITTYQEAK
                     QFFRPSLDDLHDPFLMRDMEKAVKRITEAINNQERILIFGDYDVDGTTAVALMYAFFY
                     SRYRNIDFYIPDRYKEGYGISFQGIDYAKEHQCTLMIALDCGIKANDKAAYAKELGID
                     LIICDHHLPGDILPDAYALLDPHRPDCSYPYKDLSGCGIGFKVVHAFAKYHDIPVEQV
                     ISYLDLVVVSIASDIVPIMGENRILAYYGLEQLNKNPRPGLKALIDITGYTPPLNISN
                     IVFGLGPRINAAGRMDDANQAVKMLISENVDAAKNGADVLQEKNVDRKEVDKMITADA
                     LDILATDPLTPGRVSTVLRSSNWHKGVIGIVASRLLDHYYRPTIMLAEKDGMLFGSAR
                     SVKNFDIYEAIKACSDLLEQFGGHMYAAGLSMRQENFEAFRDKFDHVVRERIRPEQLI
                     PEIEIDSTLELKDITPSFFNIVRQFAPFGPGNMEPVFISEQLMNTGRSKIVGDGHLKL
                     AVRDSRRFTADGIAFQKGHHFPLIADGKRFDICYTIEENTFRDMTNIQLNVRDIKVNV
                     "
     gene            6610..7383
                     /gene="lptB"
                     /locus_tag="PLM90_01230"
     CDS             6610..7383
                     /gene="lptB"
                     /locus_tag="PLM90_01230"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HMZ88015.1"
                     /translation="MQSMIVRAEKLIKKYKQRTVVNEVSVEVNQGEIVGLLGPNGAGK
                     TTSFYMIVGLIRPLSGKVFLDNMDITSFPMYKRAQLGLGYLPQEASVFRKMSVENNIA
                     SVLQMTNLSHKEQKAKLESLLDEFGLKHVRKSNGDVLSGGERRRTEIARALATDPKFI
                     LLDEPFAGIDPIAVEDIQLIVEKLKFKNIGVLITDHNVQETLSITDRAYLLFEGKILK
                     QGTAEDLAGDPQVRKVYLGQNFVLRKSRATTNTTQPPVE"
     gene            7472..8935
                     /locus_tag="PLM90_01235"
     CDS             7472..8935
                     /locus_tag="PLM90_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ88016.1"
                     /translation="MERIHSVVSDPIRAQDQVFQTLIDGGRDTVWGKQYDYASLRSLN
                     DLKNRFPVQDYDTLKPYIDRIMQGEQQVLWHSHIAWFAKSSGTTSDKSKFIPVSSMAL
                     EECHFRGGRDVIAMYLHNNPESKLFDGKSLLMGGSHQINKLNQHSKYGDVSAVMMQNL
                     PWAAKYAMTPSLEIALMDEWESKIEAMAHYTKNDNVTSIAGVPTWTIVLIRRLFELCG
                     TDNVKDIWPNLELYIHGGVSFKPYRSQFNTLIRKDNMHYVETYNASEGFFAFQDRLGA
                     DDMLLMPDYGLFYEFIPMEEFDKEDPKTLSWGEVELGKNYAVVVSSNAGLWRYKIGDT
                     IRFTSLYPHRVQVSGRVKHFINAFGEEVIVDNSDKAIAEASAQTGAKVVEYTAAPIYI
                     SGNEKGGHEWIIEFSTPPENIEQFTAILDATLKSINSDYEAKRHKDLALKMPVVHIAR
                     KGAFYDWLKSKGKLGGQHKIPRLSNERTYVDEILKFL"
     gene            9015..9758
                     /locus_tag="PLM90_01240"
     CDS             9015..9758
                     /locus_tag="PLM90_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ88017.1"
                     /translation="MQRYTAILVDDEEHGRRNLQTLLEKYCPEIEVIAEAAGAAEAKE
                     KILSLTPQVLFLDINMPELNGFDLLESIAKKDFAVVFVTAHRDFGIEAVKAGATDYLL
                     KPIVIKELQQAVKKVVMYYDERSAEANNADVPKPERITLGHTGGFSVVEMKDIVRLEA
                     ESNYTRVHVTGKKSYFVSKPLKVFEDSLKDNIFFRVHRSYIINLNHVKEFLREDGGVI
                     VMLDDARIQLPKARYNDFLEAMRKLSIAI"
     gene            9769..12654
                     /locus_tag="PLM90_01245"
     CDS             9769..12654
                     /locus_tag="PLM90_01245"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ88018.1"
                     /translation="MTLKSFICLVFICIGMHVKAQIFPARQYTTRDELANSNVYDICH
                     DDRGYLWFATERGVSRFDGYKFTNYLFKEGLDGAIVFSLSKDARGNILAATKFDGAFK
                     MSGNFNKIIEEDLLLANEQTVLAGKYFYSLKDGVRVAVYQMGNNKTSVLSFPGGAKPY
                     FILQKDSSAVYIGTSSGVFISKNGKTPERVFPEINEPVHSLAIRGNTVFVGLQGAVYA
                     IADNAVSVAALVPDGGKVKRLMVDDNNRIWCATFPENRLYMFDKNTAIDISQKLNIAG
                     ISVNKILEDNEGNVWIATYGKGVFCLHHMYCTNYTSYDGLKNEYITSLLPIEHELLIG
                     TYNGLFGNTNNQITPLKSFNNELEFIRKLDIHDDLIIATVSGISADKVLIKRSTIGNH
                     PLQYVHATTTFIDGNYIYYSRWDKKVWRAQINGTEIGDAEVVFEDKEGEWTRVNAIFR
                     DPQNKLWVGTTRGVYIISTNNSWVRNDTGFLRTNITAFALDHDGSVLLGTDKGLVRYK
                     DGKWIASHEINGKNLENITGLVHDNNDRIWISTLNGLFLMAENELIQFDARNTLLSDE
                     INALAYDASENAIWVGTSFGLSRIDVGLFDKAVVIAPAAIFKTLRAADSIYRDLGING
                     RLELPYTSKNLSVRFSAIQFAAPEGIKFFYKFDDGPWEPTTGRQIEFASIPYGEHTIQ
                     LKSVGERGVEGPVANLTILVTTPIWATAWFKLLIGLTIAGIAYFLLRKRFEIQRKKQQ
                     ERLELQSKVAELRHQALAASMNPHFIFNALNSIQHFINSHNTEEATDYLGKFARLIRM
                     MLDYGGRTFIPLKDELERLEYYLELEKVRFGEKLNYSIEVDDSLRNNPLEIPNMVIQP
                     VVENALWHGLLPANRNGHLLVKFAQRDNVIFVTVDDDGIGIIESKRRKKSSHNSLGIQ
                     MIRERLELLNRLSGYIAEIEIKDKSEINTDKEGTIAQIVMGGKKEQQ"
     gene            12915..15179
                     /locus_tag="PLM90_01250"
     CDS             12915..15179
                     /locus_tag="PLM90_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMZ88019.1"
                     /translation="MQIMEANRTIALQNGVLLSGDNDWVGAILHTSDTFYNCNVRLKG
                     DWVDHLQGDKWSFRIKLGSDDAINGMRVFSVQDPITRDHLSEYVYHQWLAAEDILTTR
                     YDFMPFTLNKGKTYTYAVEEHFDINLIESQHRRAGPILRFSEEFLWDLRVQDSAAFKS
                     GGMYENNIDVFAITDILSFSDKGLGKDVELTKQYEVARTLMQQYRAGKVALADIFDMD
                     KMAKYFAIVDINQAYHSLIWHNMRFYFNPITQKLEPIGFDGFTEYGVYDNIKRPFIGY
                     SASYQYRQKIEDDALTDRFFNDFEFSNTYAKYLHKFADTNYINAFFDSIGESLTEKGN
                     LIRKDYPEYQYNKKHIYDRTLAIEINLKPQGDYTLRAFADMAAPAKIGIINHVAVPVS
                     VLGMSIRPDTIMYYLDKPINLPAWNEYKAPPFAAVKKIGESQYLFYKVAGTDSLVSVK
                     ISPWEDDLVQTPEQRLFNTTISSNELYVIKNNEIFFKPGLHVVTKPIIFPAGYNIHIS
                     PGTQLDLRNGAMFVSRSPLSVIGTESSPIKIFSSDSTSMGFTVLQAASASAFQYVIFD
                     HLSALDHEGWKLMGAVNFYESTVSLEHCRFTHSQCEDALNIMRSNFSIDYSYFANTYG
                     DAFDSDFSTGTVKNTYLTHTGNDALDFSGSFIEVKRCELVHLGDKGMSCGEDSKITIS
                     DCSIKDARIGVAAKDLSRVEVQNLQISDSGIGYLAFVKKQAFGKSSIQVTGGTLTNVK
                     QTTSVLDGCEIIMQ"
     gene            15278..15811
                     /locus_tag="PLM90_01255"
     CDS             15278..15811
                     /locus_tag="PLM90_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HMZ88020.1"
                     /translation="MPYLCTTQKRGHQKVPSFVSVTMGNREQIEEWLGPKLEELGCFL
                     VDIRINPSSSKYEVYIDTPTGVTIEQCEKVSRHLSFMIENQGGFPEKYTLDVSSPGMD
                     NPFKVQQQYDKTIGKTVEVLLLSGVKMEGMLKKADGKVLHLEVHHPPKKKGMKPEITL
                     ETYDFSEIKHVKKKINF"
     gene            15877..17118
                     /gene="nusA"
                     /locus_tag="PLM90_01260"
     CDS             15877..17118
                     /gene="nusA"
                     /locus_tag="PLM90_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMZ88021.1"
                     /translation="MNSIELVESFSEFKDVKNIDRPTLMKVLEDIFRTLLRKKYESDE
                     NFDIIVNTEKGDLEIWHRREIVEDGMVENPVTQISLADALKIEPDFSVGEETYEEVSI
                     ESFGRRAILAARQALVQRIMELEKDEVYKKYKDREGEIITGEVYQVWKKEILLLDDDG
                     NELILPKGELIHSDNYKKGDAIRGVVKKVDFRNNNPVVIVSRTDPKFLEKLLEQEVPE
                     VFDGLIVIRRIVRAPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDI
                     INWTNNNQLLIQRALSPAKITSIELDNDRKKAAVYLAQDQVSLAIGKGGLNIKLACSL
                     TGYDIDVFRDNEEADVDIDLDEFADEIDEWIIDELKAIGCDTAKSVLELSVDELVRRT
                     DLEEETIKEIVRILKAEFDED"
     gene            17181..19985
                     /gene="infB"
                     /locus_tag="PLM90_01265"
     CDS             17181..19985
                     /gene="infB"
                     /locus_tag="PLM90_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMZ88022.1"
                     /translation="MSEEKSIRLAKAASEFNVSLAHIVDLLKTKGFSVEPKPTSKISE
                     EMHNILLKEFGKDKDLKGKADQLNIGIGRKLEKEKQEKEEAEALQEKPVQEVVVEEEK
                     VVAPKVTKEKKVEEEVKAPEPEPEPEPEPEAPAKEEKDELIKMERGKVGLTVVDKIDL
                     NKKKAPKKTVAPEEKPVVEKAKSDKKEKVEEVKPVVEKQEIPVAKQVTEVPEKKPEAE
                     MIETKRVTLEGPKIIGRIEIKEEKKPEKRGPDNNAEKKKRKRIIKHERINVDKVKEEE
                     RQRENRNRHQGGQGTRKPEVDSEEAQRQIQEQIRQTLAKLTGGGQQKGRGSRMAKQMK
                     RERREAAEIEAEAASHSNQLMVAEFTSLSELASLMNVSPTSLVQSCFNLGMMVSINQR
                     LDAEIIELLAEENGFKVQFINATEEETEEDDIPDDPADLETRPPIVTIMGHVDHGKTS
                     LLDYIRETNVVAGEKGGITQHIGAYEVDLPNGKRIAFLDTPGHEAFTAMRARGAKLTD
                     IAVIVIAADDQVMPQTKEAISHSQAANVPMIFAINKIDKPGANSDKIKEQLSQMNILV
                     EDWGGKFQSQEISAKSGLNVETLLEKILLEAEILDLKANPDKEAVGTVIEASLDKGRG
                     YVSNIMVQEGTLKVGDMMVAGPFFGKVKAMFNERNQRITEAGPSTPLLVLGLNGAPQA
                     GEKFKIFEDETQAKSVANKRSQILREQGIRTKKHITLEEIGRRRALGTFRELNIIIKA
                     DVDGSTEALTDSLQKLSTKEVQVNVIFKGVGQITESDIMLASASDALIVGFQVRPSSS
                     VRKLAEKEGVEIRLYSIIYNAIEEVKAAMEGMLAPKMEEKVICMVEVRDTFKISKVGT
                     IAGCYVTEGKITRNTKIRLVRDGIVIHTGSLSSLKRFKDDAKEVTTSMECGLTINNYN
                     DIQPGDMIEGYEEVEVKRSL"
     gene            20474..20686
                     /locus_tag="PLM90_01270"
     CDS             20474..20686
                     /locus_tag="PLM90_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMZ88023.1"
                     /translation="MSNVLLLSMPGGMEWVFIILAVLLLFGGKKIPELMRGLGKGIRE
                     FNDAKNNVSREIREGMNEEPKKSEEK"
     gene            20702..22147
                     /gene="gatA"
                     /locus_tag="PLM90_01275"
     CDS             20702..22147
                     /gene="gatA"
                     /locus_tag="PLM90_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HMZ88024.1"
                     /translation="MSAVSDSLLRSGLHAVQADLRAGTISVRQIVEHYLRVNRDKQHL
                     NAFLEIFEHDALHQADEIDARVRSGKPMGKLFGMVIGVKDVISIKGKRLTASSKVLDD
                     FESLYNATVIDRLRHADAIILGRCNCDEFAMGSSNENSAFGPVLNDLDNTRVSGGSSG
                     GSAVAVQAGLCMATLGSETGGSIRQPASFTGTIGFKPTYGRLSRYGLIAFASSFDQIG
                     PITNTVEDMALMMEAMAGQDPHDATSSPEPVSHYSEHLDAPKKLRIAYYPQTLDNPKI
                     DPEISAAMYGLIDKLRADGHVVESCDLKYLDYLVPTYYVLTTAEASSNLSRFSGIHYG
                     FRDMEAEDMESVIRRSRSKGFGKEVQRRIMLGTFVLSSGYYDAYYTRAQKVRRIIRDD
                     TAETLSSYDLILLPTTPTTAFKLGEKTTDPIEMYLADIFTVQANITGLPAISVPLFRH
                     SNGMPFGAQLMGNYNSEELLFSVTSYLLKNY"
     gene            22359..23909
                     /locus_tag="PLM90_01280"
     CDS             22359..23909
                     /locus_tag="PLM90_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ88025.1"
                     /translation="MDHLDSLLEAFYDSNPAWRFDEQDYALNGLAVTDTPRFTPEVIA
                     DRISKINSPIPYTYNKSVQTFIDLYTIKRRTQVSNMLALSQVYFPMFEEELDRRGMPL
                     ELKYLPVIESALNTHAVSKAGATGLWQFMLGTAKLKGLRIDTYVDERRDPHMATIAGC
                     QYLQDLYNIYGDWLLAIAAYNCGPGNVNKALRKAGGGDKTFWDIEQYLPKETKAYVPI
                     FIAATYTFEYHKEHNIRPATFGYDFSMSDTLMITHKMTIDQLAPYVGISADEIALNNP
                     ALKTKTIPGSPVPYPLILPQKAVAAYYANKDSLYASLERKEQIASQQLAKNVAAVNAA
                     NDKLKPKPATVAGTGDSSVTTATATPINTTTPDLTLADPDAPVTVNYTVKKGDNLGYI
                     SDWFDCTVADLKKWNKLSSTQIVPGQKLKITVPAKYEQQYAQINNMTLSQKQKLTDIA
                     LISPASTTDKGPVVTNDIIFYTVKPGDTLWSISQAYPENSIDKIRELNDMGKDETLKT
                     GVKIKLVK"
     gene            24019..25749
                     /locus_tag="PLM90_01285"
     CDS             24019..25749
                     /locus_tag="PLM90_01285"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMZ88026.1"
                     /translation="MHQVSLFIRRFSISALLMLLVFSAQGQTDWTFKKEVVDSVLELQ
                     YFSTQMPGYAMCIIKDGKIVYTGVRGKSSIKLNQKMSSTTQFNIGSVTKQFTAAAIYL
                     LEEEGKLSLSDNVKKYIPEFPDYPTAITIDQLIHHTSGIRDHIEVATLLSKYKQDLNE
                     LDGMLSWIQKYPELNAAPGTSFAYSNTNYMLLAVIIERLSGMKYEDFLDTQIFKPLGM
                     QATYVERGKHKTLRDGTTHYDINYKQTHAKPADAIQNAPGATGVITTLEDLVKWDNNF
                     YHNILGKKNQSLINKMEQSAVLVDGSDTHYGAGLLVTNYRFKPVIEHSGGWGQYLTQY
                     RRFPDEGVTVIVCTNAYLMSPFVMCDNVCNTLFNYKNLHLFTQNKIPEIVSKQICGPY
                     AAENNLIRYIYTKNNNLYIARIAEGDTTAYPMRYVGPMDNGYAFMDTTGKSISIVMEG
                     NKPHHLSWDGGTYFGAMRNYQYIDTTLEINFYKYHGKYYCAALDKKIRIQYFSKEDEL
                     TIHPFPFIKYNLISKGGDYYQVEGQPYIVHFGNGHLLIGNDWVYNIRADKKIKRHIDL
                     WPFEKKSSEK"
     gene            complement(25769..28942)
                     /locus_tag="PLM90_01290"
     CDS             complement(25769..28942)
                     /locus_tag="PLM90_01290"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88027.1"
                     /translation="MRKYILFIVSLLLIPATYAQTNISGVINIYTVVDSVNNCSNQVY
                     TTSAAGFSVGDRVLLIQMKGAQVNLTNTASFGNLTAYNNAGNYELATISAISGSVISF
                     QNGILRNYTALSAIQLVRVPVYGDVNIVGTLSAADWNGSTGGILAFKADMVTFSADID
                     VSGKGFRGGNAGNYPSSCPTGTGTSLYYSDTLSGKGGAKGEGIVILTSGYLACRGKAF
                     NGGGGGNDHNAGGGGGANGGGGGVAGENDEAFFSCPGSPGFASLAPDQTMAADKLFLG
                     GGGGAGHGNNNNGSDGGDGGGLVLIEANQIIGNSHSIISNGVTVPSMAWGDGAGGGGA
                     GGTIALVCPSVSALTISANGGKGGDTGADQCTGPGGGGSGGVLKHYGATIWPGVIVNL
                     NGGTFGTNITATSDCFGLNNGAAAGSTGSTISNFIAAESITPYVADFANAGSDVTVCN
                     GGSIMLNASGGVSYSWSPATYLDNPAISNPTCTPLATTNYVVTVTNANGCTDIDNITV
                     TLAPPAVANAGDDISICSGESTTLNGSGGVSYSWSPSIFLDDAFAANPIASPLIDIIY
                     TLTVTDINGCVGTDEVAITVNSSNFLTTSGDVDACGGGDVMLHAVGADSYLWTPSTYL
                     DDPTSANPICSPLTDITYTITAISTEGCVDVDSLSVNIIPAAFLNAGDDVSTCTGADV
                     ILNATGGVVFHWSPSTYLDDADIASPTCTPLSDIIYIVTSETADGCTDTDTISVSVLP
                     SDFADVDATSPVCTGTSTTLHASGGTTYNWSPSTYLDDATVANPVCTPLTDITYFVSV
                     TNADGCVDVDTVTVNTIPSPVITAGPDTTVCFGGQIKLFVTEGVAYNWTPATFLDYTT
                     IESPTASPTATISYIVYVTDASGCIGSDTVDIIVNSIPDITASADITICRGDTATLTA
                     VGGVEYTWTPDPLIPCIDCSSIDVAPTSTTTYIVQGIDANGCSATDEVTVTVDICDAI
                     NNILAEEIQIFPNPTTGNFQISCPKSANFQSLELFNLIGEKVNCEVKSDQSNITVNVA
                     ESHSGQLLLLIRFDEGTFVEMITILGE"
     gene            29007..30044
                     /locus_tag="PLM90_01295"
     CDS             29007..30044
                     /locus_tag="PLM90_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ88028.1"
                     /translation="MANKLGINVKMFIFFMLLQQGAFSQAIPADSMFSLIKQYNAYGA
                     GADWASIDKQFHQSLISAQTSDDTIQSLIHVFATLDDVHTTFQINGKTYGFYHGVTAE
                     ESAQLQPLMQRQREESGKIFTTKLPGDIAYIRIPTLNIWGDDIHAYTKTIQDSLLNIL
                     DKNTIGCIVDLRLNGGGNMYPMLAGLYPLLGNNTVMHITNPDSTVATTWFLKKGNLYG
                     ENIYGDKVPVTNVDYTSKENYDNLKVMVLIGPVTMSSGQATAVAFYKRKNTLFIGEPS
                     ATGYATAKNYYPLPNNCFMNMSSGFISDREGELYPQVVNPQLWVIHGDNFDKLSEDLK
                     VQMSTWRLTDK"
     gene            30076..31263
                     /gene="kbl"
                     /locus_tag="PLM90_01300"
     CDS             30076..31263
                     /gene="kbl"
                     /locus_tag="PLM90_01300"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HMZ88029.1"
                     /translation="MYTIQDRLQHEIQDIKESGLYKNERIITSPQGADITLADGSKVV
                     NFCANNYLGLSSHPKVIEAAKKAVDSHGYGMSSVRFICGTQDIHKTLERKISEFLGTE
                     DTILYAAAFDANGGVFEPLFGEEDAIISDALNHASIIDGVRLCKAVRYRYNHNDMADL
                     EVQLQAAQAQRSRIIVTDGAFSMDGTIAALDQIVALAEKYNAMIMIDECHASGFLGKT
                     GRGTHEYRGVMGKIDIITGTLGKALGGASGGFTSGRKEVIELLRQRSRPYLFSNTLAP
                     SITGASIAVLDLLSETTELRDKLENNTMYFREKMTAAGFDIKPGVHPIVPIMLYDAVL
                     SQQMAAKLLEEGIYVIGFYYPVVPKGQARIRVQISAAHDQHHLDKAISAFVKVGKELG
                     VIK"
     gene            complement(31280..32374)
                     /locus_tag="PLM90_01305"
     CDS             complement(31280..32374)
                     /locus_tag="PLM90_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ88030.1"
                     /translation="MASMIEQPGNEQEKQINKQFSYTRIAAVIALSLGISAYLIYTSF
                     DYKAISHIQWAQDAFGWLAFGALMMVARHIGYMYRLRLITDNKLSWYQSFNVISLWEF
                     SSAITPSTVGGAAIAIYFMKKEKLSLGKSAATSMLTIFLDQVFLAAGGLILFVLLGGN
                     AMFATDAQCRSQSELPMMSAFHNMQTIYFLGYGLFAIIISLLAYGLFINAHAFKSLLS
                     SLFSLPILKKWRNDALQTGDDMILTSHELKHKSKGFWWKLFISTAITWIAFFMIPICI
                     VHAFFDPTLADLPVIFSRMFAIWMLMLLPISPGGAGIAELTFSAMMCDFTDPALVATL
                     ALLWRLLSFYPYLLLGVVILPRWVNRKYNK"
     gene            32517..33032
                     /locus_tag="PLM90_01310"
     CDS             32517..33032
                     /locus_tag="PLM90_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88031.1"
                     /translation="MHRFYACILLLLATCSMSSGLVMYLRTTHARREVIVQYSRTENQ
                     PETTILKLSGNEFTALEKERISEKIFEIQWKGERYDIIDLQYQQNGDVYLTAHHDKKE
                     AKAIKDFADAHQDSANAGNLIFFSFCDGIIHTDIIHFIPSEIFLHSEYNVILHRHSIS
                     PTSPPPDFLAA"
     gene            33061..35100
                     /locus_tag="PLM90_01315"
     CDS             33061..35100
                     /locus_tag="PLM90_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88032.1"
                     /translation="MTLFRNGLLVLATMYLYASVCFAQTDTVTLDRIVVTSFKDTRLR
                     ESSLTITPITLSDIAQRGAFNISDALAKTPGLSQLGTGNAISKPVIRGLYGNRVLILL
                     SGMRFDNQQWQDEHGLGLSEVGIGSVEVIKGPFSLLHGSDAVGGVINIIEEDKSDPFH
                     IDQDVSVKFNTNTLGEMVQYGYKRNDGAHWIGIRLAAESSPDYSDGNGTRVLNSRYDG
                     YYLKGNYGFKKGNWISDNHYMGSFNRYGFIFSDIRDFFETPDARFSRSFPGPHHIVAL
                     NIGTSENTIILPKSVLKVNVGIQSNARLEDEGSGSISLFMHLVTGQYATRWTKSLNQR
                     NTLIIAHNASIENNTNYGKKVIVPDAWMAEGNLAAYVSHSSGKLIFETGIGGGDRYIK
                     TLYTAGVNSPEKDIDPFSRHWLFANVLAGLTFNPNNNWNIKSHVATGVRAPNLAELSS
                     NGLHEGVFTYEIGDPNLHTEKNLSLAADINRTGKYITASISGYYNFFADFIYLSPTDE
                     EWFGFPIYRYLQQSAQVYGAEAFISFRIPAFPYLTYSITTADVEGKLKDGAYLPFIPA
                     LKVTPEIKFKGLHDEHNWYAFVNTDIYSPQHQTATDEIATPGYTLLNAGVGGEWHIGK
                     YPLEINLVGNNLLNEAYYDHLSRFKSLGYLNMGTNIQVHAIMHFQSTFKYHNKTN"
     gene            35106..35537
                     /locus_tag="PLM90_01320"
     CDS             35106..35537
                     /locus_tag="PLM90_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88033.1"
                     /translation="MKHFMKLNTLIIAVAAITFALASCNKDEMEPPIISFKTGAGYTS
                     DDITIAAGSSVLIGIEASKAEAEGEEEDVLSHFNISLSVNGGASTSVYDADMTAAQEE
                     EYSYDYNAAASTTVGEVDTYTFTITNRDGLTGQVSLTVTSN"
     gene            complement(35609..36358)
                     /locus_tag="PLM90_01325"
     CDS             complement(35609..36358)
                     /locus_tag="PLM90_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMZ88034.1"
                     /translation="MQNQQTFEIKLPQFEGPFDLLLFFIERDELDIHDIPISRITKDF
                     LDYLHSMTQMNIEIASEFILVAATLMRIKAKMLLPRKELDEFGNEIDPRKELVDRLLE
                     YKRYKEVLGHLSELEDDRQLRINRGNIPIEHDILAKKHSNEMELSSVNLFVLLKVFQK
                     VMDRYNNQPKVQEHVIVQYNYSIEEESFGVLSYVRGKKNVPFADVFNSCKDKYQAVFR
                     FLSILDLIATQKVTLLVGEGINNFWLTSDQD"
     gene            complement(36453..38654)
                     /gene="recQ"
                     /locus_tag="PLM90_01330"
     CDS             complement(36453..38654)
                     /gene="recQ"
                     /locus_tag="PLM90_01330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HMZ88035.1"
                     /translation="MDVSHLTLKDALQEYFGFDSFKGNQEEIITSLLNGQDTFVIMPT
                     GGGKSLCYQLPAMMLEGTAIIVSPLIALMKNQVDLVRSYSSNNDIAHFLNSSLNRQQI
                     KKVKADIVAGRTKMLYVAPETLRKEENIEFFRGIRVSFVAVDEAHCISEWGHDFRPEY
                     RQIHSMIEAINDDIPIIALTATATPKVQSDIKKNLDLEEPNVFISSFNRPNLYYEIRP
                     KRSREQAIKQIVKYIKEQGRKSAIVYCLNRKSTEDIANILNINGIKAGAYHAGLDAAL
                     RSEVQDKFLMEGYQVIVATIAFGMGIDKPDVRYVIHFDIPKSIENYYQETGRAGRDGL
                     EGKCIAFYSYKDIEKLEKFMRDKPLSEREMGAQLLAETAAYAETSVCRRKFLLFYFGE
                     EYKQENCGSCDNCLKPKEKIEGKEFVVNALKTIDVINENHHIKYVVDLLTGKRTTENQ
                     NYRHDKIELFGVGKEKDDQFWNSIIRQCLLSELVYKDIEQYGLIKLTDKGRNFIKKPH
                     SIMVSLNHDYDQEVADVDMLESNAPKSVLDPVMMDILVKLRDKVAKDAGLPPYVIFSE
                     NSLEEMATMYPTVMDEMVKISGVSRGKAEKYGRKFLEAIKKYVEENDVEKPSEFVMKQ
                     VVNKSAHKVKIIQFIDKKMPLPDIAKSIGLNFPDFIDEIETIVSSGTKLSLNYYLDDI
                     IESDLQEEVHDYFIEMSSLDLVSAYNDLKDEGLSFEEVRLLHIKFMSEMAN"
     gene            38814..>39300
                     /locus_tag="PLM90_01335"
     CDS             38814..>39300
                     /locus_tag="PLM90_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ88036.1"
                     /translation="MSNVYVVIMAGGIGSRFWPESRVKKPKQFLDILNTGETLIQTTY
                     TRFLKIADKDNIFIVTNEQYISLVNEQIPGLNPLNILAEPSRKNTAPCIAYACHVIAQ
                     RDPEATVVVAPSDHLMLNPDKFLLTVLEALEYADSHDVLLTLGIKPTRPDTGYGYIQY
                     EE"
BASE COUNT        11797 a         7761 c         8563 g        11179 t
ORIGIN      
        1 tcatgatgtg caagggaact tgaacttcca gccttatgga aaaccagggg aagcaattaa
       61 ttcggtatcg cgaggcggtt taaatgtgat gatgattgat gcagcagaaa aggccggagt
      121 tacctatttg ttcgaacagc gtattatgga aatagatcca tcaggtaaaa aagcgatgat
      181 ggaagatatg tccaccggta gacattatac cctggaaccc gacttgatca ttggtgctga
      241 tggggcctac agcctcgtgc gctatgccat gcttaaaaca gatcgatttg attatagtca
      301 ggcttatgaa gcacacgggt ataaagagtt gacaattccc gccggaccga atggaagttt
      361 tctgatagac cgcgatgctt tgcatatctg gccaagaaag cagtttatgt tgattgcact
      421 gcctaatatc gatggaagtt ttacttgtac cttgttcttt tcgttagaag ggaaggagtc
      481 gtttgaggcc attaaaaccc atgctgatct tgatgctttt ttcgatacct atttccctga
      541 tgtaaagtca ttgatccccg accttcacca cgatttcttc gcaaacccta ccggattgct
      601 gatgatcgta aaatgcttcc catggagtca cggtaacacc atgctgattg gagatgcggc
      661 tcatgccatt atcccatttt atggacaggg aatgaattgc ggtttcgaag actgtaccgt
      721 tttagataca ctgattgagg aatttaacca cgattggagc aagatcatgc cggcctatga
      781 gcgcctacgg aagccaaatt ccgatgccat tgccgacctc gccaaattaa atttcattga
      841 aatgcgtgat cttgtggcag atgccgattt tcaattcaag aagcggatcg cagccaaggt
      901 gagtgcgctt caccccgatc gtttcatacc ggtttacagt atggtgtcat ttagtcacct
      961 accttacaag gatgccctaa gtgaatatca tcgtcaggat gcggttttaa ccgaaatttt
     1021 ggggtggcct gagattgaaa ataggtggga aaccgactac cttccacaga tcgcagagag
     1081 actgtttaat aaatagtaaa tatgtaattg attggttgtc aatgttttaa atagaccacc
     1141 tttcgcgtct aagacctttc tcgtcttaaa cctaacaggt cttaaactta acaggtttaa
     1201 gacctgttag gtttgatgga ttgatggcat aaaaaaagcc ccttctttcg aagaggctcc
     1261 ttaaatctat ttacttcaaa ggattagtta ccgccggtag ctccgccttt tttcttgata
     1321 tcaatagtct ttttagtgtc gccacctttc acgttaacat ctactttttt gtcaggagca
     1381 ggtgttacag tagtggtaga ttttgttgaa gtgcttgatt tgcttgatgt tgagctcttc
     1441 atagcatcca ttttcatcca ctcttccatt tccatatgaa gagcagcaat tgaatccatt
     1501 aaagctgttt tttcagcagc acaagcgcta tcgccaacat gcatttgttt ttctaaatca
     1561 gcgatttttt gttcagcttc tttgtctgaa cagctggcaa gcacgaagcc tccggctaat
     1621 acgaacaata aaaatttgat cttcatatgg tctgagttta aaatttggaa tggtatttga
     1681 ctacaaaggt attaataatc aaacatcata ccgtaatttt gctcaacttt ttaatcaaat
     1741 caagatttat gaagactaaa atctccgttt tatccctcgc cgtaatagct gccggtggca
     1801 tgttcatcac cagctgcggt actgaagact tgtcagttcc tgtaatcgtt ctcgaaggcg
     1861 attctccttt tattgttgaa cttggcgata cctatactga tccgggcttt acagctacag
     1921 acgatgagga tggcgatatc actgcaagtg taacagttga tgacagcgat gttgatactg
     1981 aagaaatcgg cgaatatgaa gttacttata ccgtaactga tggcgccgga aacgtaggca
     2041 ctgaaacgcg tattgttcgt gttgttatgg gtaaagccga ctacatggga actttccagg
     2101 tgcatgaaat ctgcgacatg gatggtgatg gcgtttatgg tgaacccggc gttgactacg
     2161 aaatcaacga ctataccgta actgttacct ccggtggcga cgacaatgaa gtgcttttcc
     2221 aaaactttgg agcttatggc gctactgtaa tcgttcctgt ttacttcagc ggcgatctga
     2281 atgagatcct gactgtagac gattacaacc tgccgggaac taccatctac tttaacgctg
     2341 atggcgaaat cactaccggt actacttccg acattgaatt cgacctcgac tacaatgctc
     2401 aggatggtgc aactatcatc ccttgtcagg ctacattcgt gaaattgtaa tatccacgga
     2461 ccgcttcatg cggaaataaa agactcaaag ccccggctgc taaccgctcc ggggcttttg
     2521 catgaatatc attaccttag cctttctgct atgcgtaaac tgttgccaat atctatcgta
     2581 attattttct ttacctctgc ctgcacgagg gacgacagca ctgcgcccct gattcttata
     2641 cagccggatg cgcatgcacc acattaccga acaataccct tcgtagaccc cggttgtgaa
     2701 gttgccgatg attattcgtg tgccgatgcc attgatttac aaatttccaa taatgtagac
     2761 gtaaataaat atggcaccta taatatcatg tataccgcaa cggatgaagc cggtaatata
     2821 aatgaataca atcgtccggt tgatattatt ttgcctattt ccgattatta tgatttcgca
     2881 tattcggcat acgatacttg tacctccgga aattattttt ataccggatt agctcaagat
     2941 tgcgactgtc cggatgataa agttatcttc ggaaatatca gcaacttcgg accaaatgca
     3001 acttttccgc tccccataaa cggccaatat aatcacatga ttaccctcga cacaactaaa
     3061 ttggcagtca cattttttgg cacaggtgtt atgagtcctg ctgctgatac cattttttgg
     3121 gaatatgtaa ttatggattc catttctacg gatgcatgtc gttcggtttg gataaaatag
     3181 atcttttccg atcaattttt gttataacaa catgacaaca ttgaatacaa ttttcagccg
     3241 catatttctc ggtatattaa ttaccgtaag tcattcggtg tttgctgctg atcccggtcg
     3301 tatcaatggt caaattattg tgcaatttta ttcaaacgtt gaacctgaac gtgtaattat
     3361 cgatgtggag cttgcatcaa aaactgacgt tgcattatta aaaccgcttt ctgcatcttt
     3421 gaatatctgg ttaattgcct tcgatgaaaa tgtgtataat gcagaattcg taaaggatgc
     3481 tttctttaaa aatccggatg tggcattggt gcaattcaat catacatttt ctgagcgcag
     3541 cattcccgat gatccttttt tctatcagca atggggcctg ctaaatgatg gtgcaggggg
     3601 aattgcagat gcagatattg atgccgatct tgcatgggac atcacaaccg gcggcgttac
     3661 ggcaaaaggt gatactatag ttgttgcact gatcagcgaa gggcaatttt atgcacatga
     3721 agatctcgat tattttatca atagaaatga aattccggat aatgatattg atgatgacgc
     3781 caatggttat atcgatgatt atcacggctg gaatggcact actcaaaccg atagcgttcc
     3841 cgaaagattg catggcactc acgtagccgg cattgcaggt gcaataggaa ataatggatt
     3901 aggtatttcg ggtgtttctc agaagctgaa aatattacct gtatataacc ttactgttga
     3961 agcagatgct gtggcgtctt atgcatacgc ccgcgacatg cgaaaaattt ataacgaaac
     4021 aaatggcgaa aaaggcgctt atattgtcgc aacaaattct tcgttcggtg tagatttcgg
     4081 aaatccggat gattacccta tttggtgtgc tatgttcgat agtcttggtg ttgttggtgt
     4141 attaagtgta gcttcaactg ctaatacgtc ggtgaatgtc gataatgtat acgacatacc
     4201 tactgcttgt ccgtctgaat atctgatcac agtaaccatg actgataaag cagataactt
     4261 attttcggca gcgtatggtg caaaaagtat tgatcttggt gctccgggga atttgatcta
     4321 ttcaacaatt attgataatt cctatggcat cctaagtgga acatcaatgt ctgcaccaca
     4381 tgtaacaggt gctattgcta tgatgtatgc agcggcctgc cctgagtttt ttgatgcact
     4441 tgaggataac aatgaattga tgctgctcaa attaaaacaa tatctgctcg aaggcgtgga
     4501 tgttgttccg gatcttgaag gtgttacggt aagcaatgga agattaaatg tttttcgcgc
     4561 tatagaaaat ttaaccgtga gtggtttttg tgcaaccggt tttgcggaaa atgcactggg
     4621 tgtagcctac ccaaatccgg cacaggaatt attatttatt aacggcgcaa caattattga
     4681 tcaggaagtg caagtgcaag ttgtaaatac actcggacaa gtggtttacc agcgtaccgg
     4741 tgatcgctca ccatttatat tttcaggaat ccctgtcggc gatctgcctt cggggtatta
     4801 tgctgtttct gtatacaacc cggattctga tttattattc gggtcgggtg ttataattca
     4861 acatcaataa attttatttt cgtgctatgg acaaacgatg ggtgattaaa gtgacggagg
     4921 aggaaaaagt tcagtccctg caacaggagt tgaaaataca tcctgtaatc tgcaatttgt
     4981 tggtgcaacg tggcattaca acatatcagg aagcaaaaca gttttttaga ccttcattag
     5041 atgatctgca tgatcctttt ttaatgaggg atatggagaa ggcagtaaaa cgcattactg
     5101 aggcaattaa taatcaggag cgcattctca tttttggaga ttatgatgtt gatggcacca
     5161 ctgctgttgc gttgatgtat gctttttttt attcgcgata tcgcaatata gatttttata
     5221 ttccggatag atataaggaa ggatacggta tttcctttca gggaatagat tacgcaaaag
     5281 aacatcagtg cacgctcatg attgcacttg attgcgggat aaaagctaat gacaaggcag
     5341 catatgcaaa agaattaggc atagacctca tcatatgcga tcaccacctc cccggagata
     5401 ttctccctga tgcctatgcg ttgttagatc cgcatagacc ggattgttcc tatccatata
     5461 aagatttaag tggatgtggt atcggattta aagtggttca tgcatttgca aaatatcacg
     5521 acatccccgt tgaacaagtt atttcgtacc tcgaccttgt agttgtttca attgccagtg
     5581 atattgttcc cataatgggt gaaaatcgca tactcgctta ctatggtctt gaacaattaa
     5641 ataaaaaccc tcgtcccggt ttaaaagcac ttatcgatat caccggttat acgccaccgt
     5701 taaatatttc aaatattgtg ttcggacttg gtcctcgaat taatgctgcc ggcagaatgg
     5761 atgatgccaa tcaagcagtg aaaatgctta tttccgaaaa tgtagacgct gcaaaaaatg
     5821 gtgccgatgt attacaagaa aaaaatgtcg acagaaaaga agtagataaa atgatcactg
     5881 ccgacgcgtt agatattttg gcaacagatc ctttgacacc gggtagagta tcaactgtgt
     5941 tgcgcagcag taattggcat aaaggtgtca ttggtattgt agcatcgcgt ttgcttgatc
     6001 actattacag accaaccatc atgcttgccg aaaaagacgg catgctcttc ggaagtgcgc
     6061 gttcggtaaa gaatttcgac atctacgaag caattaaagc ttgcagcgat ctgttagaac
     6121 aattcggcgg gcatatgtat gcagcagggc tttctatgcg acaggaaaac tttgaagctt
     6181 tccgcgataa atttgaccat gtagtgcgcg aacgcattcg tcctgaacaa ttaattccgg
     6241 agatagaaat tgattctacc ttggagctta aagatattac gccgtcattt tttaatatag
     6301 ttcgtcagtt tgctcctttt ggtccgggga atatggagcc cgtttttatc agcgagcaat
     6361 tgatgaatac cggtagaagc aaaatagttg gcgatggtca cctcaaactt gccgttcgcg
     6421 acagcaggcg tttcaccgca gacggcatcg cctttcaaaa gggtcatcat tttcctttaa
     6481 tagctgacgg aaaaaggttc gatatttgct ataccattga agaaaatacc tttcgtgata
     6541 tgacaaacat acaattgaat gttcgtgata ttaaggtaaa tgtgtaggca tcattactga
     6601 atgtaaatat tgcaaagcat gatcgtaaga gcagaaaaat taattaagaa atataaacaa
     6661 cgcacagtag taaatgaggt aagcgttgaa gtaaatcaag gagagattgt aggacttctc
     6721 ggacccaatg gcgccggtaa aacaacttcg ttttacatga tcgttggatt gatacgtccg
     6781 ctttcaggaa aagtttttct ggataacatg gatatcacct cttttccgat gtataaacgt
     6841 gcacagcttg gtttgggata tcttcctcag gaagcaagtg tttttagaaa aatgagtgta
     6901 gagaataata ttgccagtgt attgcaaatg accaacctct cgcataaaga gcaaaaagca
     6961 aaacttgaat ctttgcttga tgaattcggc ttaaaacatg ttcgcaagag taatggtgat
     7021 gtgttaagtg gaggcgaacg aagaagaacc gaaattgcca gagcattggc taccgatccg
     7081 aagtttatat tgctggatga gccttttgcc ggtatcgatc cgatagcagt tgaagatatt
     7141 cagcttattg ttgaaaaatt gaagttcaaa aatatcggag tgctcattac agaccataat
     7201 gtgcaggaaa ccctctcaat tactgataga gcatatttac tttttgaggg taaaattttg
     7261 aaacagggca cggcagaaga cctggcaggc gacccacaag taagaaaagt ttacttaggg
     7321 cagaatttcg ttctgcggaa aagtcgcgct acaacgaaca ctacccaacc tcctgtggaa
     7381 taatctatac ctttattctg taagccaccg tatttttgcg aagcgaatgc caattatcca
     7441 cgccatagcc tctcgtgttt ttctccgtaa tgtggagaga atccactcag tggtgtcgga
     7501 ccctattcgt gctcaagatc aggtatttca aaccctgatc gatggcggcc gagataccgt
     7561 gtggggtaaa caatacgatt atgcctcgtt gcgatcgcta aacgacctga aaaacaggtt
     7621 tcccgtgcag gattatgaca ctttaaagcc ttacatagac cgtatcatgc aaggcgaaca
     7681 acaggtgctg tggcatagtc atattgcctg gtttgcaaaa tcctcgggca ccaccagcga
     7741 taaaagcaag tttattcccg ttagcagcat ggctttggaa gaatgtcatt ttcgcggtgg
     7801 cagagatgtt atagccatgt acctgcataa taatccggaa tcaaaacttt ttgatggtaa
     7861 gtcgttgttg atggggggca gtcatcaaat caacaaactc aatcagcatt ctaaatatgg
     7921 cgatgtaagt gcggtgatga tgcaaaattt gccatgggct gcaaaatatg ccatgacacc
     7981 atcgcttgag attgcactaa tggatgagtg ggaaagcaag atcgaagcca tggcgcatta
     8041 cacaaaaaat gataatgtga catccattgc cggtgtgcct acatggacaa tagtattgat
     8101 aaggagattg tttgaattgt gtggcaccga taatgtcaaa gacatttggc caaatctgga
     8161 attatatatt catggtggcg taagttttaa accgtatcgc agtcagttca atacccttat
     8221 tagaaaggat aacatgcatt atgttgaaac atataatgcg agcgaaggat tttttgcatt
     8281 tcaagacaga cttggtgcag atgacatgtt gttgatgccg gattatggtc tgttttatga
     8341 attcattcca atggaagaat tcgacaaaga agacccgaaa acactgagtt ggggtgaagt
     8401 ggaattaggc aaaaattatg cagtagttgt atcctcaaat gccggtttgt ggcgatataa
     8461 aatcggcgat accattcggt ttacttcgtt atatccacat agagtgcagg tttcagggcg
     8521 tgtgaaacat tttattaatg ccttcggcga agaggtaata gttgataaca gcgataaagc
     8581 tattgcagaa gcaagtgccc aaaccggagc aaaagttgta gaatatactg cggcacccat
     8641 ttatatttca ggcaatgaaa aaggtggtca cgaatggatt atcgaattca gtacccctcc
     8701 cgaaaatata gaacaattta cggccatcct ggatgcaaca cttaagtcga tcaatagtga
     8761 ttatgaggca aaacgtcata aggacctcgc actcaaaatg ccggtagtac atattgccag
     8821 aaaaggtgct ttttatgatt ggttgaaatc aaaaggcaag cttggcggac agcacaaaat
     8881 accacgccta agcaacgagc gtacctatgt ggatgaaata ttgaaattcc tctgatcgct
     8941 tcatcgccgt taggcaattc ctcttaatat ttttgctaac tttgataagg tcctatgact
     9001 ttaaacagca cttcatgcaa cgatatacag ctatactggt agacgacgag gaacacggtc
     9061 gccggaacct tcaaacatta ctcgaaaaat actgtcccga aatagaagta atcgccgaag
     9121 ctgccggtgc tgccgaagcg aaagaaaaaa ttctgtcctt aactccacaa gttttattcc
     9181 tcgatatcaa tatgcctgaa ctcaatggct ttgatttgtt ggaatcgatt gcaaaaaaag
     9241 atttcgcggt ggtatttgta acagcacatc gcgattttgg tattgaagca gttaaagccg
     9301 gtgccaccga ttatctgctc aaacctattg taataaaaga attgcaacag gcggttaaaa
     9361 aagtggtcat gtattacgac gagcgcagtg cggaagcaaa taatgccgat gttcctaaac
     9421 ccgaacgtat cactttagga cataccggcg gttttagcgt agtggaaatg aaagatatcg
     9481 ttcggttgga ggcggaaagc aattacaccc gcgttcatgt gacaggaaaa aaatcttatt
     9541 ttgtatctaa gccgttgaag gtttttgaag atagcctcaa ggataatatt ttcttccgtg
     9601 tacatagatc ttacattatc aatctgaatc atgtaaaaga atttttgcgt gaagacggag
     9661 gcgtaattgt tatgcttgat gatgcacgaa ttcagttgcc aaaggcgcga tataacgact
     9721 ttttggaggc aatgcgcaaa ttgtctattg ctatctaatt cctcattcat gactttgaaa
     9781 tcatttattt gcttagtatt tatttgtatt ggcatgcatg tcaaagcgca gatatttcct
     9841 gcaaggcaat atactacaag agatgaattg gcaaacagta atgtgtatga tatctgtcat
     9901 gatgatcgtg gatatttatg gtttgccaca gagcgcggag taagtcgttt tgatggatac
     9961 aaattcacca attatctatt taaggagggg cttgatggtg caattgtatt tagcctttca
    10021 aaggatgcga ggggtaatat tcttgctgca acaaagtttg acggggcatt taaaatgtca
    10081 ggaaacttca ataagatcat tgaagaagat ttgttacttg ccaatgaaca aacagtattg
    10141 gcgggtaaat atttttattc attaaaagat ggtgtacggg tagccgtata tcaaatgggt
    10201 aataacaaaa cttcggtatt gtcgtttcct ggcggtgcta agccatattt cattttgcag
    10261 aaagatagca gtgcagtata tataggcacc tcaagcggcg tttttatttc taagaatggt
    10321 aaaactccgg agcgtgtatt ccctgaaatt aatgagccgg tccatagcct ggccattcga
    10381 ggaaatacgg tttttgttgg tttgcaaggc gcggtttatg cgattgctga caatgctgtt
    10441 tcagtggcgg cgttagtacc ggatggtggt aaagtgaaac ggttgatggt tgacgacaac
    10501 aacagaattt ggtgcgccac ttttcctgaa aatcgattgt atatgttcga caagaacacg
    10561 gcgattgata tttctcagaa attaaatatt gcaggtatct cggtcaataa gatattggaa
    10621 gacaatgaag gaaatgtttg gattgcaacc tatggaaagg gagtattttg tttgcatcat
    10681 atgtattgta ctaactacac gtcgtatgat ggtttaaaaa atgaatatat cacttcgcta
    10741 ttgccaattg aacatgaatt attgattggt acatataacg gtttgtttgg aaatactaac
    10801 aatcaaatca caccactaaa atcatttaac aatgaattag aattcattcg caagcttgat
    10861 atacatgatg atctgattat tgctaccgtt tcaggtattt ctgctgataa agtacttatc
    10921 aagcgatcaa caatcggcaa tcaccctttg caatatgtgc atgcaactac cacttttatt
    10981 gatggcaatt atatttatta tagtcgctgg gataaaaaag tgtggagagc gcaaataaat
    11041 gggactgaaa taggtgatgc agaggttgtg ttcgaagaca aggagggcga gtggacacga
    11101 gtgaacgcaa ttttccgcga cccccaaaat aaactgtggg ttggtactac aagaggtgtt
    11161 tacattattt caacgaataa ttcttgggta aggaatgata ccgggttttt acgcaccaat
    11221 atcaccgcct ttgctttgga tcatgatgga tctgttttgc ttggaacaga caaagggctc
    11281 gtcaggtata aagatggtaa atggattgct tctcatgaaa taaatggtaa gaaccttgaa
    11341 aatataacag gtctagttca tgataataac gatcgcattt ggataagtac attaaatggc
    11401 ttgtttttaa tggctgaaaa tgaacttatt cagtttgatg ccagaaatac cttgttgagc
    11461 gacgagatta atgctttagc ctacgatgct tcagagaatg ccatctgggt gggcacaagt
    11521 tttgggttgt cgcgtattga tgttggatta ttcgacaaag ctgttgtaat tgcaccggcg
    11581 gccattttta aaacattgcg agcagccgac tcgatttatc gcgatcttgg tattaatggc
    11641 aggttggagt taccctatac ttcaaaaaat ttgagcgttc gattttctgc cattcagttt
    11701 gcagctccgg aagggataaa attcttttat aaattcgatg atggtccctg ggagccgact
    11761 accggtcgtc agattgaatt tgccagcatt ccatatggtg aacatactat tcaattaaaa
    11821 tcggttggag aacgtggagt tgaagggcct gtagcgaacc tgaccatttt ggtaaccacc
    11881 ccaatctggg cgaccgcctg gtttaaattg cttatcggcc ttactattgc aggcatagca
    11941 tatttccttt tgcgaaaacg atttgaaata caacgcaaaa agcaacaaga gcgactcgaa
    12001 ttgcaaagta aagttgctga gctgcgtcac caggctctgg cagctagtat gaacccgcat
    12061 tttattttta atgcactcaa ttccattcaa cattttatca atagccataa tactgaggaa
    12121 gctaccgatt atctcggcaa gtttgcgcga ttgattcgca tgatgctgga ttatggtggt
    12181 cgcacattta ttccgctcaa ggatgagctg gagcgattag aatattacct tgaattagaa
    12241 aaagtgcgat ttggcgaaaa gttgaattat tccattgaag tggatgacag tttgcgaaat
    12301 aacccactcg aaataccaaa tatggtcata caaccggttg ttgaaaatgc tctttggcat
    12361 ggattgttgc cggcaaatag gaatggtcat ttgctggtca aattcgcgca acgcgataac
    12421 gtcatctttg taacagttga tgatgacggt atcggaataa tagaaagcaa gcgaagaaaa
    12481 aagtcatcac ataactcctt gggtatccaa atgattcgtg agcgacttga actattgaac
    12541 cgcttgagtg gatacatcgc agaaattgaa ataaaggata aatcggagat caacactgat
    12601 aaagaaggaa caattgccca gatcgtcatg ggagggaaaa aagaacaaca ataaatattg
    12661 ttccaattgc ggtcgattga tcgcaggaac ctatatgaca ttattgctta agcagttttg
    12721 gttacattaa tttaacaatg ctacctttgt ccactcttat taattacatt aatagctttc
    12781 atgaaatcaa tctactcagg ctacttgtta ttggctgcca ttacgatagt tacattattt
    12841 gatgcttgcc ggccggtaca acctgatcaa cgacatcaga taacggtctt acattagata
    12901 tatcacccga gggtatgcaa ataatggaag ccaacagaac aattgcgctc caaaacggcg
    12961 ttttgttatc aggtgataac gattgggttg gtgccatact ccacacaagc gatacttttt
    13021 acaattgcaa tgtcaggtta aaaggtgatt gggttgatca tttgcaagga gataaatggt
    13081 cgtttagaat aaagctcgga agcgatgacg ccattaatgg tatgcgcgta ttttctgttc
    13141 aggatccaat tacaagagat catttatctg aatatgttta tcatcaatgg cttgctgctg
    13201 aagacatttt aactactaga tatgatttca tgccatttac gttgaacaag ggtaagacat
    13261 atacttacgc agttgaggaa cattttgata tcaacctgat cgaatctcaa caccgaaggg
    13321 ccggtccgat attgagattt tcagaagagt ttttatggga tctaagagta caagatagtg
    13381 cagctttcaa atccggcggg atgtacgaaa ataatattga tgtatttgct atcaccgata
    13441 ttctttcatt tagtgataag ggattgggga aagatgttga gcttaccaaa caatacgaag
    13501 tggcgagaac attaatgcaa caatatcgcg cagggaaggt agcactggcc gacatttttg
    13561 atatggataa aatggcaaaa tatttcgcaa ttgttgacat taaccaggct taccatagtt
    13621 taatatggca taatatgcga ttttatttta atccgataac tcaaaaactc gaacctattg
    13681 gatttgatgg ctttacagag tatggcgttt atgacaatat caaacgcccg tttatcggat
    13741 attccgcttc ttatcaatac cgtcagaaaa ttgaagatga tgctttaacc gatagatttt
    13801 tcaacgactt cgaattttca aatacttacg caaaatacct gcataaattt gcggatacta
    13861 attatataaa cgcttttttt gattcaatcg gcgaatcgtt gactgaaaaa ggcaacttaa
    13921 ttcgaaagga ttatcccgaa taccaataca acaagaaaca tatttatgac cgtactctgg
    13981 cgattgagat taacctcaag cctcagggag attatacgtt gcgcgcattt gcagatatgg
    14041 cggcacccgc taaaattgga ataataaatc atgttgctgt tcctgtatca gttttgggca
    14101 tgagtataag acctgatacc atcatgtatt atcttgataa accaataaat cttcccgcct
    14161 ggaatgaata taaagcaccg ccttttgccg cagtgaaaaa aattggagaa agtcaatact
    14221 tgttttacaa agttgcggga acagatagtc tggtgtcagt taaaatttct ccatgggaag
    14281 atgaccttgt tcaaacgcct gagcaaagac tatttaatac aacaatttct tccaatgaat
    14341 tgtatgtaat taaaaataat gaaatattct ttaaaccggg attacatgta gttacgaagc
    14401 ctataatctt tccggcaggt tataatattc atatttcacc cggaacacaa ttggacctgc
    14461 gcaatggagc aatgttcgtt tcaagatcgc cgttgagcgt tatcggtaca gaatcatctc
    14521 caattaaaat attttcatca gattctacct ccatgggatt tactgttttg caggctgctt
    14581 cagcatccgc atttcaatat gtgatttttg atcatttatc cgctttggat catgaaggtt
    14641 ggaaattaat gggagcagtt aatttttacg aatcaaccgt atcattggag cattgccgat
    14701 ttacgcatag ccaatgtgaa gatgccttga atatcatgcg ttcaaatttt agcatcgact
    14761 attcctattt tgcaaatacc tatggcgatg cgtttgattc tgattttagt acaggcacag
    14821 taaaaaacac ttacctcaca catactggta atgatgcgct tgatttttca ggtagtttta
    14881 ttgaagttaa aagatgtgaa ttagtccatc tcggcgataa aggtatgagc tgcggcgagg
    14941 actcaaaaat tacaatatcg gactgttcta tcaaggatgc acggatagga gtagcagcca
    15001 aggatttaag tagagtagaa gtacaaaacc tgcagatctc agattcaggc atcggatatc
    15061 tggcttttgt taaaaaacaa gcctttggaa aatcctctat tcaggtgact ggaggcacgc
    15121 tcaccaatgt aaagcaaacc acttcagtgc tcgacggctg tgaaattatc atgcaatagg
    15181 ccggttttag ctgatttcag ccatttcagg tcactctaag cggaccggaa atgccctttg
    15241 gggggcgctt tcaacacgat ttgcacattt cggtgcaatg ccctatcttt gcaccactca
    15301 gaagagggga caccaaaaag tcccctcttt tgtttctgta accatgggta accgtgagca
    15361 aatagaagaa tggctggggc ctaagctgga ggagctgggt tgttttttgg tggatatccg
    15421 catcaatcca tcgtcatcaa agtatgaggt atatattgat accccaacag gtgttacgat
    15481 tgaacaatgc gaaaaggtta gtcgccacct ctccttcatg atagagaacc agggaggctt
    15541 tcccgaaaaa tatacacttg atgtgtcgag tccgggaatg gataaccctt tcaaggtgca
    15601 gcagcaatac gacaaaacca ttggcaaaac ggttgaagtg cttctgcttt ccggcgtgaa
    15661 aatggaaggc atgcttaaga aagcagatgg taaagtgctg catttggaag tgcatcatcc
    15721 gccaaagaaa aaaggcatga aaccggagat aactttagaa acctatgatt tcagcgagat
    15781 aaaacacgtg aaaaagaaaa tcaattttta actcaaataa acagcaacac cgcttcatcc
    15841 gggattttcc ggacgaggca ttaaaaatca agtgctatga acagcataga actagtagaa
    15901 tccttctcgg agtttaaaga cgtcaaaaac attgaccgtc ctacattgat gaaagtgttg
    15961 gaagacatct tccgtacctt acttcgtaaa aagtatgaaa gcgatgagaa ctttgacatc
    16021 atcgtaaata cagaaaaagg tgacctcgag atctggcatc gccgcgagat cgttgaggat
    16081 ggaatggttg agaatcctgt tacacaaatc agcctcgccg atgccctgaa aatagagccc
    16141 gacttttcgg taggtgaaga aacctatgaa gaggtgagca tcgaaagctt cggtcgccgc
    16201 gctatcctgg ctgcacgtca ggctttggtt cagcgtatca tggagcttga aaaagacgaa
    16261 gtttacaaga aatataaaga tcgtgaaggt gagatcatca ccggcgaagt ttatcaggta
    16321 tggaaaaaag aaatcctcct gctcgacgac gatggcaatg agttaatact tcccaaaggt
    16381 gaactaattc attccgacaa ctataaaaaa ggtgatgcta tacgcggtgt tgtgaagaaa
    16441 gttgacttcc gcaataacaa tccggtagtt attgtttctc gtaccgatcc aaaattcctc
    16501 gaaaaattac tcgaacagga agtacctgaa gtatttgatg gattgatcgt gatacgccgc
    16561 atcgttcgtg ctcccggtga gcgtgcgaaa gttgctgttg agtcatacga cgatcgcatc
    16621 gatccggttg gcgcttgcgt tggtatgaaa ggatctcgta ttcatggtat cgttcgtgaa
    16681 ttgcgcaacg aaaacattga tattatcaac tggaccaaca acaaccagtt gctcatccag
    16741 cgcgctttaa gccctgcaaa aattaccagt attgaactgg ataatgatcg caaaaaggct
    16801 gctgtttacc tcgcgcaaga tcaggtttcc ctggccatcg gaaagggtgg attgaatatt
    16861 aaattagctt gttcactcac cggttacgac atcgatgtct tccgcgataa tgaagaagca
    16921 gacgtagata tcgacctcga cgaatttgca gatgagatcg acgaatggat catagatgag
    16981 ctcaaggcta tcggttgcga tactgccaag agcgttctgg aactgagcgt tgatgaactg
    17041 gttcgccgca ccgaccttga ggaggaaacc attaaagaaa tagttagaat attaaaagca
    17101 gagttcgacg aagattaaga ttccacaggg tgctgtaaaa gcatcacgcg ataatcctat
    17161 ctttgtagcc acaatactga atgtcagaag aaaaatccat acgattagca aaggccgcga
    17221 gcgagtttaa cgtatctctt gcccatattg tcgacctgct gaaaaccaaa ggtttctcag
    17281 tggagccgaa acctacttcc aaaatttcgg aagagatgca caatatcctg ctcaaggaat
    17341 ttggcaaaga caaagacctg aaggggaaag ccgatcaatt gaatatcggt atcggccgca
    17401 aactggaaaa agagaaacaa gagaaagaag aagcagaagc cctgcaggaa aaacctgtac
    17461 aagaggtagt tgttgaagaa gaaaaagttg ttgctcctaa agttaccaag gaaaaaaagg
    17521 ttgaggaaga agtgaaagct cctgagcctg aacccgaacc ggaaccggaa cccgaagctc
    17581 ccgcaaagga agagaaagat gagttgataa aaatggagcg cggcaaagtt ggcttaacgg
    17641 ttgtagataa aatagatctc aacaagaaaa aagcgccgaa aaaaactgta gctcccgaag
    17701 aaaaaccggt agttgaaaaa gctaaatctg acaaaaaaga aaaagtagaa gaggtaaaac
    17761 ctgttgtaga aaaacaggaa ataccggttg caaaacaggt aacggaagtt cctgaaaaga
    17821 aaccggaagc agaaatgatc gagactaaac gcgttacact ggaaggtcct aagatcattg
    17881 gtcgcattga aatcaaagaa gaaaagaaac ctgaaaaacg cggacctgat aataatgcgg
    17941 aaaagaaaaa acgcaaacgc attattaagc atgaacgcat caacgttgat aaggtaaaag
    18001 aagaagaacg tcaacgcgaa aacagaaaca gacatcaggg tggtcaagga accagaaaac
    18061 ctgaagtaga ttctgaagaa gcacaacgtc agatacagga acaaattcgt caaacacttg
    18121 ccaaacttac cggcggcggt caacaaaaag gtcgcggttc gagaatggca aagcaaatga
    18181 aacgcgagcg tcgtgaagct gcagaaattg aagcagaagc agcgtcgcac agcaatcagt
    18241 taatggttgc cgaatttact tcgctttcag aattggcgag tcttatgaat gtatcgccaa
    18301 cttcactagt gcaaagctgt ttcaacctcg gtatgatggt ttccatcaat cagcgtcttg
    18361 atgcagagat catagaattg ttggccgaag aaaatggatt caaggttcaa tttatcaatg
    18421 ccaccgaaga ggaaacagaa gaagacgata ttccggatga tcctgcagac cttgagacaa
    18481 gaccaccgat agttaccatc atgggtcacg ttgaccatgg taaaacatcg ttgctcgatt
    18541 atatccgcga aacaaatgta gttgccggtg agaagggtgg tatcactcag cacatcggtg
    18601 cttacgaagt agacctgcca aatggcaaac gcatcgcatt cctcgatacg ccgggtcacg
    18661 aagcctttac cgccatgcgt gctcgcggag cgaaacttac tgatattgca gttattgtaa
    18721 ttgctgccga tgaccaggtg atgcctcaaa caaaagaagc catcagtcac tcgcaagcag
    18781 caaacgtacc aatgatcttc gcgatcaata agattgataa accgggtgct aattccgata
    18841 aaataaagga acaactcagc cagatgaata ttctggttga agattggggg gggaaattcc
    18901 aaagtcagga gatatctgca aaatcaggtc ttaatgttga aacacttctg gaaaaaattc
    18961 tccttgaagc agaaattctc gacctgaaag ctaatcctga taaagaagct gtaggtaccg
    19021 ttattgaagc atcattagat aaaggacgcg gatatgtatc caatatcatg gtgcaggaag
    19081 gaacactgaa agttggtgat atgatggttg ccggtccatt ctttggaaag gtaaaagcca
    19141 tgttcaacga acgaaatcaa cgcatcactg aagcaggtcc ttcaacacca ttgctggtat
    19201 taggtttgaa tggtgctcct caagcgggtg agaaattcaa aatatttgaa gacgaaaccc
    19261 aggcaaaatc tgtagccaac aaacgcagtc agatcttgcg cgaacagggt atcagaacca
    19321 aaaaacatat caccctcgaa gagatcggtc gccgtagggc attgggtacc ttcagagaat
    19381 tgaatatcat catcaaggcc gacgttgacg gttctacaga ggcgcttacc gacagcttac
    19441 aaaaactgtc taccaaagag gtacaggtta acgttatctt caaaggtgtt ggtcagatca
    19501 cagaaagcga catcatgctc gcatctgctt ccgatgctct gatcgttggc ttccaggtac
    19561 gtccaagcag ctctgtccgc aaactggctg agaaagaagg tgtggagatc agactttaca
    19621 gcattatcta caacgctatt gaagaagtga aagccgccat ggaaggcatg cttgcaccga
    19681 aaatggaaga aaaggttatt tgtatggtcg aagtgcgcga tactttcaaa atttcgaaag
    19741 tcggcactat tgcaggctgt tacgttaccg aaggcaagat caccagaaat accaaaattc
    19801 gtctcgtgcg cgatggtatc gtaattcata ccggttcatt gagctcactg aaacgcttca
    19861 aagatgacgc gaaggaagtt acaaccagca tggagtgcgg tcttaccatc aacaactaca
    19921 acgatattca gccgggcgat atgatcgagg gttatgaaga ggttgaagtg aaacgttcac
    19981 tgtaatctgc tactagggaa ccaaagttcg gttcattctg catacgaaat aaaaaaaggc
    20041 gacatggagc agtcgccttt ttcatgatgg ataccatcat cttttaggga atcatcaacc
    20101 cgaatcagat cccgaaaaaa tttgtgtttt cacgaagtgc tttcatgggg tgatagcact
    20161 tgtgtttacg ggatagtgtt ttcagtagga tcgaggtcat attgacggca ttttggcctt
    20221 cacaacaacc cactttgagt gaaatgacct aactattgag tgaaatgtgc tatctattga
    20281 gtgaatgagg gcgatttttt aggcgaaatg acaccttttc gtgatgaaat gtgtcggttt
    20341 gaatgttgaa taagcttgct gatagttaaa attgcctgta atctgcatcg tagctcctgc
    20401 ggttgatgag aattgcttta tgcaggattt ggtattcatc ttttgtgtac ctttgcattc
    20461 taatcaagta tagatgagca acgtattatt gctttcaatg cccggcggaa tggaatgggt
    20521 atttatcata ttggctgttt tgcttctgtt tggcggcaaa aaaattccgg aactgatgcg
    20581 tggacttgga aaaggtatcc gcgagttcaa tgatgccaaa aacaacgtaa gtcgcgagat
    20641 tcgtgagggt atgaatgaag aacctaaaaa atctgaagag aagtaattac tgtctttcca
    20701 gatgtctgct gtttcggatt ctttgctgcg ttccggttta cacgccgtac aggctgacct
    20761 gcgtgcgggt acaatttctg tgcgtcagat cgtagaacac tatctccggg taaacagaga
    20821 caaacagcat ctcaacgctt ttcttgaaat ttttgaacac gatgcactgc accaggcaga
    20881 tgagatcgat gcacgtgtgc gctccggcaa gccgatgggt aagttgttcg gaatggttat
    20941 cggtgttaaa gatgtaatat ccataaaggg taagcgtctc accgcatcat caaaggtact
    21001 cgacgatttt gagtcattgt ataatgctac agttatcgat cgattgcgac atgctgatgc
    21061 catcattctt ggtcgctgca actgcgatga gtttgccatg ggttccagta atgaaaacag
    21121 tgcttttggc ccggtattaa acgatcttga taacacacgt gtttccggtg gcagctccgg
    21181 aggttcagct gtggcagttc aagccggact ttgtatggca actttgggct ctgaaacggg
    21241 agggagtatc cgtcaacccg catcctttac cgggactatc ggttttaaac ccacttatgg
    21301 acgcctttca cgatatggtt tgattgcatt tgcttcttca tttgatcaga ttggtccaat
    21361 caccaatact gttgaggata tggcattgat gatggaggct atggcaggac aggatccgca
    21421 tgacgccact tcatctcccg aaccggtgtc acattattca gagcacctgg atgcacctaa
    21481 aaagctgcgt attgcctatt acccgcaaac ccttgataat cctaagatag accctgagat
    21541 cagcgctgca atgtatggat tgatcgacaa attgcgtgct gatggtcatg tggtggagtc
    21601 ctgtgacctg aaatatctcg actatctggt tccgacctat tatgtgctta ctacggcaga
    21661 agcatcatca aacctcagca gattttctgg cattcattac gggtttcgtg acatggaagc
    21721 ggaggatatg gagagcgtga ttcgccgttc cagatcgaag ggttttggca aagaagtgca
    21781 gcgaagaatc atgctcggca cctttgtttt gagctcagga tattacgatg cttattacac
    21841 cagggcacaa aaagttcgac gaataattcg cgatgatacg gcagaaacac tttcgtcgta
    21901 cgaccttatt ttactgccta ccacgcccac tacggcgttt aaactaggtg agaaaacaac
    21961 cgatcctatc gagatgtacc tcgccgatat ttttaccgtt caagccaaca ttacaggcct
    22021 gcctgccatc tctgtccctt tatttagaca tagcaacgga atgcccttcg gagcccagtt
    22081 aatgggcaat tataacagtg aagaactgct cttttctgtg acatcgtacc ttcttaaaaa
    22141 ttattaaaat ttaggtgtgg atattgattt atccgctttc tgacgcaata aaacggtaat
    22201 tttatagttt tatagccctg aaaccattgc ctaaaccgag aaaaacgagg gaataacgaa
    22261 tgcacttttt gagaattacc gtatgctgcc ttttgctggg gggtatagca aaagcacagg
    22321 tctctaattc tccaactctc aacccttctg atgagagtgt ggatcatctt gacagtctgc
    22381 tcgaggcgtt ttacgatagt aatccggcct ggaggtttga tgaacaggat tatgctttga
    22441 atggcttagc cgttacagat acaccgcgat ttactcctga agtgattgct gatcgcatca
    22501 gcaagatcaa ttcccccatt ccatatacct acaacaagtc ggttcaaacg tttattgacc
    22561 tgtataccat caaacgtcgt acgcaggtga gtaatatgct ggcattgagt caggtttatt
    22621 tccccatgtt tgaggaggaa ctcgatcgca gaggaatgcc tttggagtta aaatatttgc
    22681 cggtaattga atcggcgttg aatacgcatg cagtttcgaa ggcgggtgca acaggattat
    22741 ggcaattcat gctgggaaca gctaaattga aaggattgcg cattgatacc tatgttgatg
    22801 agcgtcgcga cccgcatatg gcaacgattg cgggttgtca atatctgcaa gacctctata
    22861 atatttatgg cgattggttg cttgcaattg ccgcctacaa ctgcggtccc ggaaatgtaa
    22921 acaaggcgtt gcgcaaagcc ggtggtggtg ataaaacttt ttgggatatc gaacaatatc
    22981 tccctaaaga aacaaaagcc tacgtgccga ttttcattgc agcaacgtac acattcgaat
    23041 atcacaaaga gcacaatatt cgccccgcaa cttttggtta cgattttagc atgagcgata
    23101 ctttgatgat cacacataaa atgaccatcg atcagctagc tccttatgtt ggaataagtg
    23161 cagatgaaat tgcattgaat aatcccgcac tgaaaacaaa aaccatcccg ggatctcctg
    23221 ttccatatcc attgatactg ccgcaaaaag cagtggccgc gtattatgcc aataaggatt
    23281 ctttgtatgc atcgcttgaa cgcaaagaac aaattgcatc acaacaattg gctaaaaatg
    23341 tggctgcagt aaatgctgca aatgataaat tgaaacccaa acccgcaacc gttgccggca
    23401 caggcgacag cagtgtaaca accgcaacgg caacaccgat aaacactacc actccagacc
    23461 ttacccttgc agatccggac gcaccggtta ctgtgaatta tacagtgaaa aaaggcgaca
    23521 acttgggtta tatatcagat tggtttgatt gcacggttgc tgatcttaag aaatggaata
    23581 aattaagttc tacgcagatt gttcccggcc agaaattgaa aattaccgtt cctgctaagt
    23641 atgaacagca atatgctcag ataaacaaca tgacgctgtc gcagaaacag aaactcactg
    23701 atattgcatt gatcagccct gcatcaacaa ctgataaagg gccggttgtt acaaacgaca
    23761 tcatcttcta taccgtaaaa ccgggcgata cgctttggag tatctcacag gcttatcctg
    23821 aaaactctat agataagatc cgcgagttga acgatatggg taaagatgaa accttgaaaa
    23881 caggcgtgaa gatcaaattg gtgaaataat cgccgcctcc ctgatttttg gttgaccaat
    23941 aaaccactcg gaaccatttc gcgagcaggg aattatttgt catataattt gtcgagttgc
    24001 tgccgtaatt ttgagcatat gcatcaggta tccctattca tacgtcgctt cagtatctct
    24061 gcattattga tgctattggt tttttctgca caaggacaaa ccgattggac ctttaaaaaa
    24121 gaagttgttg attctgttct tgaattgcaa tatttcagca cgcaaatgcc gggttatgcg
    24181 atgtgtatta ttaaggatgg taaaattgtg tataccgggg tgcggggaaa gagctcgata
    24241 aaattaaatc agaaaatgtc atctaccaca caattcaata ttggttcggt gactaaacaa
    24301 tttaccgctg ctgcaattta cttgttggaa gaggagggga aactcagtct ttccgataat
    24361 gtaaaaaaat atatacctga atttcctgat tacccgacag cgattactat tgatcaatta
    24421 attcatcata cttcaggcat aagagatcat attgaggttg caacgctgtt gagtaaatac
    24481 aaacaagatt tgaatgaact tgatggcatg ttatcatgga ttcaaaaata tcccgaatta
    24541 aatgctgccc cgggaactag ttttgcttat tccaatacca actatatgtt gttggctgta
    24601 atcattgaac gtttgtcggg aatgaagtat gaagattttc tagacacaca aatattcaaa
    24661 cctctgggca tgcaggcaac ttatgttgaa agaggcaaac ataagacact tcgcgatggc
    24721 acaacacatt acgatattaa ttacaaacaa acccatgcca aacctgcgga tgctattcaa
    24781 aatgccccgg gagcaacggg tgtaattaca acattagaag atctcgtaaa atgggacaat
    24841 aatttttatc ataatatatt gggtaaaaag aatcagtcgc tgattaataa aatggagcaa
    24901 tcagctgtac ttgttgatgg gtctgataca cattacggcg cgggattgct tgtaaccaat
    24961 tatcggttta aaccggttat tgaacatagc ggaggatggg gccaatattt aacacaatat
    25021 cgcaggtttc ctgatgaagg tgttaccgtt attgtttgca ctaacgccta tttaatgtca
    25081 ccatttgtta tgtgtgataa tgtttgtaac accttattca attataaaaa tctccacctg
    25141 ttcacacaaa ataagattcc tgaaattgta agtaaacaga tttgtggacc atatgctgcg
    25201 gagaataatc ttattcgata catatacacg aagaacaaca atttatatat cgcccgaatt
    25261 gcggaaggag acactacagc ttatccaatg cgctatgtag gcccgatgga taatggatat
    25321 gcttttatgg atacaacagg caaaagtatt tccattgtta tggaaggtaa taagccacat
    25381 catctttctt gggatggagg tacttatttt ggggcaatgc gcaattatca atacatagat
    25441 accacactgg aaatcaattt ttataagtat catggaaaat actactgcgc ggctctggat
    25501 aagaaaattc gtatacagta tttttcaaaa gaagatgaat tgaccatcca cccatttcca
    25561 tttatcaaat ataatttgat ttcaaaaggg ggtgattatt atcaagtaga aggacaaccc
    25621 tatattgttc acttcggtaa cgggcattta ttaatcggca acgactgggt atataatata
    25681 cgtgccgaca aaaaaattaa aagacatatt gacctctggc ctttcgaaaa gaaatcttcg
    25741 gaaaaatagg ttgaattaaa tacgcgcctt attcgcccaa tattgttatc atttctacaa
    25801 aagttccctc atcaaatctg atcaacagaa gtaattgccc tgaatgggat tcagcaacat
    25861 taacagtgat attactttga tctgatttta cttcacaatt aactttttct ccgatcagat
    25921 taaataattc caacgattgg aaattagcag atttcggaca tgagatttgg aaatttcctg
    25981 ttgtgggatt tggaaatatt tgtatttctt ctgcaagtat attattgatg gcatcgcaaa
    26041 tgtcaactgt tacagttacc tcatcggtag cgctacagcc atttgcatca ataccctgta
    26101 ctatataagt agttgttgat gtgggtgcta catcgatact gctacaatca atacatggaa
    26161 tgagtggatc gggtgtccaa gtatattcaa ctccaccaac ggcggtcagc gtagcggtat
    26221 ctccccgaca aatagttatg tctgcagagg cagtaatatc cggaattgaa tttacaataa
    26281 tgtcaacagt atccgatcca atacatccac ttgcatcggt tacatagaca atatagctga
    26341 ttgttgcagt tggacttgca gttggacttt caattgtagt ataatctaaa aatgttgccg
    26401 gtgtccagtt ataggcaaca ccttctgtta caaataattt tatttgtccg ccaaaacata
    26461 ctgtcgtatc cggaccggca gtaataaccg gagatggaat tgtatttacg gtaacagtat
    26521 ctacatcaac acaaccgtcg gcatttgtaa cacttacaaa ataggtaata tcggtgagtg
    26581 gtgtacaaac cggatttgca acagttgcat catcaagata cgttgaaggg ctccaattat
    26641 aagtggtccc tcccgaagcg tgtaaagttg ttgaggttcc ggtacacaca ggcgatgttg
    26701 catctacatc agcaaaatcg gaaggaagta cggaaaccga tatcgtatct gtatcagtac
    26761 atccatctgc tgtttcagat gtaacgatat aaataatatc actgagcggg gtacatgttg
    26821 gcgatgcaat atcggcatca tcaagatagg ttgaaggact ccagtgaaat acaacaccac
    26881 ctgtggcatt taatatcaca tcggcacctg tgcatgttga aacatcatca ccggcattta
    26941 aaaatgcagc aggaataata tttaccgaaa gagaatctac atcaacacaa ccttcagtac
    27001 ttattgctgt gatagtatat gtaatatcag ttagcggact gcaaattgga tttgcagatg
    27061 ttgggtcatc taaataagta gaaggagtcc ataaataact atcggcacct acagcatgta
    27121 acattacatc gccaccacca caagcatcaa catcgccgct ggtagttaaa aagttagagc
    27181 tgttcactgt tatggcaact tcatcagtac caacacatcc attaatatcc gtaacggtta
    27241 gtgtataaat aatatctatt aatggcgaag caataggatt agcggcaaat gcatcatcta
    27301 aaaatatcga tggcgaccag ctatatgaaa caccgcctga accattcaat gtagtagatt
    27361 caccactaca aatgctgata tcatcgcctg catttgcaac tgcaggagga gcaagtgtaa
    27421 cagtaatgtt atcgatatcc gtacaaccat ttgcatttgt aactgtcacc acataattag
    27481 tagtagccaa tggtgtgcat gttggatttg aaattgcggg attgtctaaa taagttgcag
    27541 gactccatga atacgataca ccacccgaag catttagcat tatactacca ccgttgcaaa
    27601 cagtaacgtc gctgccggca ttggcaaaat ctgcaacata aggagttata ctttctgctg
    27661 cgatgaaatt acttattgtg ctgcctgttg aaccggctgc tgcgccatta ttcagaccga
    27721 agcaatcgct ggtagcggta atatttgttc cgaaggtgcc gccattgaga ttgacgataa
    27781 cgcccggcca aattgttgcg ccataatgtt tgagtacacc accggagcca ccgccaccgg
    27841 gacctgtaca ttggtcggcg ccggtatcac cacctttgcc gccgtttgct gaaatggtta
    27901 aggcagaaac cgaagggcaa actaaggcaa tagtaccacc ggcaccgcca ccgcctgcgc
    27961 catcgcccca tgccatgctt gggacagtta ccccatttga aataatacta tggctattgc
    28021 cgattatttg atttgcctct atgagcacca gtccaccgcc atcaccgcca tcagatccat
    28081 tattgttatt gccatgaccg gcaccgccac cgcctccaag gaaaagtttg tcggccgcca
    28141 ttgtctggtc aggagccagg gatgcgaatc cggggcttcc ggggcaagag aaaaatgcct
    28201 catcattttc tcctgccacg ccaccaccac cgccattggc accgcctccg ccgcctgcat
    28261 tgtggtcatt tccgccacct ccgccgttga acgctttgcc cctgcacgcc aggtatccac
    28321 ttgtgagaat aacgattcct tcgcccttgg cgcccccctt tccgctgagg gtatcagagt
    28381 agtaaaggct ggttcctgtg cctgtagggc agctggaggg gtaatttccg gcattgccac
    28441 ctcgaaatcc ctttccgctg acatcaatat cagccgaaaa ggtcaccata tcggctttga
    28501 aggccagaat gccaccggtg ctgccattcc aatcggctgc actcaaggtt ccaacaatat
    28561 tgacatcacc gtacaccgga acgcgaacga gctggattgc tgacagtgca gtatagttgc
    28621 gaagtatgcc attttgaaag gaaataacag acccgctgat ggcagaaata gttgccaact
    28681 catagtttcc ggcattatta tacgcggtga gattaccgaa agaggctgta ttggtgaggt
    28741 taacctgcgc acctttcatt tgaatcaaca acacacggtc acccacactg aaaccggcag
    28801 cggaagttgt gtatacctga ttgctgcaat tattcacgga gtcgacaaca gtgtatatat
    28861 ttatcactcc gctgatattc gtttgagcgt atgtggcggg aatgagtaaa agtgaaacaa
    28921 tgaaaagtat atattttcgc ataggttgat gattcaacga taaaattaat caaatgctgt
    28981 ttttcggaac aaatctccgc ttttgtatgg ctaataagct tggaattaat gtgaagatgt
    29041 ttattttctt catgttgttg caacaaggtg cattttctca ggcaattcct gctgatagta
    29101 tgtttagctt gatcaagcaa tacaatgcct atggagcagg tgccgattgg gcttcgatag
    29161 ataaacagtt tcatcaaagt ctcatttcgg cacaaacctc agacgacacg atacaatcgt
    29221 tgatacatgt tttcgctacc ttggatgatg tgcatactac ttttcagata aatggtaaaa
    29281 cgtatggttt ttatcatggt gtaactgctg aggagtctgc acaattacag cccttaatgc
    29341 aacgtcaacg cgaagaatcc ggcaagattt ttacgacaaa attgcccggt gatattgcct
    29401 atatccgcat tccgacttta aatatttggg gggatgacat tcatgcatat acaaaaacca
    29461 ttcaggatag tcttttaaat attcttgata aaaatacaat aggttgtatt gttgatctgc
    29521 gattaaatgg cggaggaaat atgtatccga tgttggcagg attatatcca ttattaggaa
    29581 ataatacggt tatgcatatc accaatccgg atagtaccgt tgctacaaca tggtttttga
    29641 aaaaaggaaa tttatatggt gaaaatattt atggcgataa agttcccgta accaatgttg
    29701 actacacttc taaagaaaat tatgacaact taaaagttat ggtattaatt ggtcccgtta
    29761 ccatgagcag cggacaggca acagcagttg cattttataa gaggaaaaat accttattta
    29821 taggtgagcc aagtgccacg ggatatgcca ctgcaaaaaa ttattacccc ttgccaaaca
    29881 attgcttcat gaatatgtcg tcggggttta taagcgatag ggaaggagag ctgtatccac
    29941 aagtggtaaa tccgcagttg tgggtaatac atggcgacaa ttttgataag ctgtcggaag
    30001 atctgaaagt acagatgtcg acatggcgat taacggataa ataaatttca attaatttca
    30061 caccaaatta atcatatgta taccattcag gatcgtttgc aacacgaaat tcaggacatc
    30121 aaagagtcgg gtttatataa aaatgaacgc atcattactt cacctcaagg tgccgatatt
    30181 actttggctg atggaagcaa ggttgtgaat ttttgtgcaa acaattattt gggattgagt
    30241 tcacatccaa aagtaattga ggcagcaaag aaggctgtag atagtcatgg atatggaatg
    30301 agcagtgtgc gttttatctg cgggacacag gatatacata aaacattaga acgcaaaata
    30361 tcagagttct taggcactga ggatactatt ttatatgccg cagcttttga tgcaaatggt
    30421 ggtgtatttg aaccattgtt tggagaagag gacgccatta tttccgatgc tttaaatcat
    30481 gcatctatta ttgatggtgt gcgtttgtgc aaagctgtca gatatcgata caatcataat
    30541 gatatggctg accttgaagt gcaactacag gcagcacagg cacaacgcag caggattatt
    30601 gttacggatg gtgcatttag tatggatggc actattgcgg cattagatca aattgttgcg
    30661 ttggctgaaa aatataatgc catgatcatg atagatgaat gtcatgcatc agggtttttg
    30721 ggcaagacag gtcgcggcac acatgagtat cgcggagtga tgggtaaaat tgatatcatt
    30781 accggcacgc tgggtaaagc acttggcggc gcaagcggtg gatttacatc aggaagaaaa
    30841 gaagtgattg aattattgcg tcaacgctct cgtccgtatt tattttcaaa tacgttggcg
    30901 ccaagtatta caggtgcttc aattgctgtg cttgatttgt tgagtgagac cactgagctg
    30961 cgtgataagc tggaaaataa taccatgtat ttccgcgaaa aaatgacagc agccggattt
    31021 gatattaaac ccggtgttca tcctatagtg cctatcatgt tatatgatgc cgtattgagt
    31081 caacagatgg ctgcgaaatt gttggaagag ggaatttatg tgattggatt ttattatcct
    31141 gtagttccaa aaggccaggc acgcatcagg gtgcagattt ctgctgctca tgatcaacat
    31201 catcttgaca aagccatttc agcatttgta aaagttggca aagagttagg ggtaattaaa
    31261 taattgctgc aagagagtat cacttattat attttctgtt cacccatctt ggtaaaatca
    31321 ccacaccaag aagcagataa gggtaaaatg aaagcaatcg ccataataat gcgagggtgg
    31381 ctactaatgc ggggtctgta aagtcgcaca tcatggcact aaaggttaat tcggcaatac
    31441 ctgcaccacc gggacttatg ggcagcaaca tgagcatcca gatggcaaac atcctgctga
    31501 aaattaccgg cagatcggca agtgttgggt cgaaaaaggc atggacaatg caaatgggaa
    31561 tcataaaaaa tgctatccat gtgattgccg ttgatataaa taatttccac caaaaccctt
    31621 tcgatttatg ttttaattcg tgcgatgtaa gtatcatgtc gtcacctgtt tgcagggcat
    31681 cattacgcca ttttttgagt atgggtaagc tgaaaagaga tgacagcaga cttttaaatg
    31741 catgtgcatt gatgaataag ccgtaagcga gcaatgaaat tatgattgca aataaaccat
    31801 agccaagaaa atagattgtt tgcatgttat gaaatgccga catcatgggc agttcactct
    31861 ggctgcgaca ttgtgcatct gtggcaaaca tggcatttcc tcctagcaaa acaaataaaa
    31921 taagtcctcc ggcagcaaga aaaacctgat caaggaatat agtaagcatg ctggtggcgg
    31981 cgctttttcc caggcttaat ttttcctttt tcatgaaata aatagcaata gccgcacccc
    32041 ctacggtgga tggtgtgatg gccgaagaaa actcccacaa agagatcacg ttgaacgatt
    32101 gataccagct caatttgttg tcggtaatga gtcgtaatcg atacatatat ccaatatggc
    32161 gcgctaccat catcagcgca ccgaaagcga gccatccgaa agcatcctga gcccattgga
    32221 tgtgggaaat ggctttataa tcgaatgaag tataaatgag atatgcggag ataccaagac
    32281 tcagcgctat gactgcggct atgcgcgtgt aggaaaactg tttgttgatt tgtttttcct
    32341 gctcgttgcc cggttgctca atcattgaag ccatatgcct ttcgaagtta tagaaaaaag
    32401 ttgaacatga aacgttggct tatttgccca aggtaaaaca agctgcatat agcgctaagg
    32461 ttgttttaac actttttctt ttgtcaatta tacttccatt aataccttta agccttatgc
    32521 acaggtttta tgcatgcata ttattactat tggcaacttg ttcgatgagc agcgggttgg
    32581 tcatgtacct gcgcactaca catgctcgac gggaggtgat tgtgcaatat tcccgcacag
    32641 aaaaccaacc ggagacaaca attttaaagc tctccggcaa cgaattcact gcacttgaga
    32701 aggaaagaat ttctgaaaag atatttgaaa ttcaatggaa gggtgaaagg tatgatatta
    32761 ttgatttgca atatcagcaa aatggcgatg tttacctgac cgcgcatcac gacaaaaagg
    32821 aagcaaaggc aattaaagat tttgcagatg cgcatcaaga tagtgcaaat gccgggaacc
    32881 tcattttctt ctctttttgc gacggcatta ttcacactga tatcattcat tttatcccaa
    32941 gtgaaatttt cttgcatagt gaatataatg tgatcctgca taggcattct atttcaccca
    33001 cttctccacc tccggatttt ttagcggcat aaacaattat gtaacgctaa aacatacaac
    33061 atgaccctat ttcgaaatgg gttattggtg ttggcaacaa tgtatttata tgcatctgtg
    33121 tgttttgcac aaacggacac agttacattg gatcgtatcg tagtaacctc ttttaaggat
    33181 acgcgtttac gtgaaagtag ccttaccatc acacccatca ctttatctga tatcgcacaa
    33241 agaggtgcat ttaatatttc tgatgctctg gcaaaaactc cgggcctaag tcaattaggt
    33301 acaggaaatg ctatttccaa acctgtaatt cgtggtttgt atggcaatcg tgtattgata
    33361 ttgctttcgg gtatgcgatt cgataatcag caatggcaag atgagcacgg actcggattg
    33421 agtgaagtgg gtatcggcag cgttgaagtg attaaaggac ctttcagttt attacatgga
    33481 agtgatgccg taggcggtgt tatcaatatc attgaagagg ataaatccga tccgtttcac
    33541 attgatcagg atgtttctgt aaaattcaat acaaacacac ttggtgaaat ggtccagtat
    33601 ggctataagc gcaatgatgg cgcacactgg ataggcatac gcctggctgc agaatcaagc
    33661 ccggattaca gcgatggcaa tggaacgaga gtgcttaaca gtcgctatga tggttattat
    33721 ctaaaaggca attatggctt taagaaaggt aattggataa gcgataatca ttacatgggt
    33781 tcctttaatc gatatggatt tattttcagc gatatccgcg atttctttga aacgcccgac
    33841 gcacgattca gcagatcttt tcccggtccg catcatatag ttgctttgaa tatcggcact
    33901 tcggagaaca ctatcatttt acctaaaagt gttttgaagg tgaatgtcgg tatacagtcg
    33961 aatgcgcggc tggaagatga aggcagtggt tcgatcagtt tatttatgca tttagttacg
    34021 ggtcaatatg ctactcgatg gactaaatca ttaaatcaga gaaacacttt gataattgca
    34081 cacaatgcat caattgaaaa caataccaat tacggaaaaa aggttattgt acctgatgcg
    34141 tggatggcgg aagggaatct tgcggcatat gtatcacaca gttccggtaa attaattttt
    34201 gaaaccggaa ttggcggcgg cgacagatac atcaaaacct tgtatacggc aggtgttaat
    34261 tcgcctgaaa aggatattga tccgttttcc cgtcattggt tgtttgcaaa tgtcctggcg
    34321 ggattaactt ttaatcccaa caataactgg aatattaaat cgcatgttgc cactggtgta
    34381 agggctccga atctagctga attatcttca aatggtttgc atgaaggtgt atttacctac
    34441 gagattggcg atccaaatct gcatacagag aaaaacctca gccttgctgc ggatattaat
    34501 cgaaccggta aatatattac tgcttcgata agcggctatt acaatttctt tgcagatttt
    34561 atttatttat cgccaaccga tgaggagtgg tttggtttcc caatttatcg ctacttgcaa
    34621 caatcagctc aagtgtatgg tgccgaagca tttatatctt tcagaattcc tgcatttccg
    34681 tatttaacct acagcataac cactgcagat gttgaaggaa aattgaaaga tggggcatac
    34741 ctgccattta ttccggcact caaggtaacg cctgaaatta aattcaaggg attacatgat
    34801 gagcataatt ggtatgcatt tgtcaacacc gatatatact cacctcaaca tcaaacggcg
    34861 acagatgaaa tagctacacc gggctacaca ttactgaatg ccggcgtggg tggcgagtgg
    34921 catattggaa aatacccact tgaaattaat ctggtgggaa ataatttatt aaacgaagcc
    34981 tattatgatc atttgagcag atttaaatcg ctcggatatc tgaatatggg cacaaatatt
    35041 caagtgcatg ctatcatgca ttttcaatca acttttaaat atcacaacaa aacaaattaa
    35101 acattatgaa acattttatg aaattgaata ctttgatcat cgccgtagca gcgatcacat
    35161 ttgcattagc atcgtgtaat aaagatgaaa tggaaccacc aatcattagt tttaaaaccg
    35221 gcgcaggata tacctcagat gatattacca tagctgcagg ttcttctgta ttaattggga
    35281 ttgaagcatc aaaggctgaa gccgaaggtg aggaagagga tgtgttatca cactttaata
    35341 tttctttatc tgtcaacggc ggagcgtcaa catcagtgta tgatgctgat atgactgctg
    35401 ctcaagaaga ggagtattca tacgattaca atgctgctgc ttcaactact gttggtgaag
    35461 tggacactta cactttcacg attacaaaca gagatggttt aacaggtcag gtaagtttga
    35521 ctgtcacttc gaattaatag tcgcgcttaa taaaaaaaag gctgctttcg ggcggccttt
    35581 ttttatttca gaaaattgat tttaataatt agtcctgatc agaagtaagc cagaaattat
    35641 taattccttc gccaaccaat aatgttactt tttgtgttgc aataagatca agaatactca
    35701 ggaaacggaa cacagcctga tatttgtctt tacatgaatt gaatacatcg gcaaatggaa
    35761 cattcttttt gccacgaacg taagacagca caccaaaact ttcttcttca atagaataat
    35821 tgtattgcac aattacgtgt tcctgtactt tcggctggtt gttatagcga tccatcacct
    35881 tttggaatac cttgagcagc acaaaaaggt ttacgctact caattccatt tcattgctat
    35941 gttttttagc aagaatatcg tgttcgattg gaatatttcc acgattaatt ctcagctgac
    36001 gatcgtcttc cagttctgag agatgtccga gtacttcttt gtatcgtttg tattccagaa
    36061 gtcggtctac gagttcttta cgcgggtcga tctcattacc aaattcatcg agttctttgc
    36121 gcggcagcaa catttttgct ttgatacgca tgagggttgc tgcaacaaga atgaattcac
    36181 tggcaatctc gatattcatc tgcgtcatag agtgcaggta atcgagaaag tctttggtta
    36241 tgcgtgaaat cggaatatcg tgaatatcca actcatcgcg ttctataaag aataacagca
    36301 gatcgaacgg cccttcgaac tgtggtagtt tgatttcgaa tgtttgctgg ttttgcatac
    36361 gtattggttg tcaaaggtag tgtgtgttgc gaattccggc aagcgacttt ctaaaaggaa
    36421 aaaaattgcc ggtatcgatg cttgtcacac tattaattgg ccatttcgct cataaatttg
    36481 atgtgtaaca atcgcacttc ttcaaatgat agaccttcat ccttcaagtc attgtaagcg
    36541 gaaacgaggt cgaggctgct catttctatg aaatagtcgt gcacttcttc ctgcagatcg
    36601 ctttctatga tatcatcaag ataataattc aagctcaatt ttgtgcctga ggatactatt
    36661 gtttcgattt catcaataaa atcagggaaa ttcaagccta ttgatttcgc gatatccggc
    36721 aatggcattt tcttatcgat gaattggatg atcttcactt tgtgtgcaga tttattaacc
    36781 acctgcttca tcacaaattc cgaaggcttt tctacatcat tttcctctac atacttctta
    36841 atagcttcaa gaaacttcct gccatatttt tctgcttttc ccctgctgac gcctgaaatt
    36901 ttcaccattt catccataac agtcggatac atggtagcca tttcttcaag agagttttcg
    36961 ctgaagatca cataaggtgg cagtccggcg tctttcgcaa ctttatcgcg aagcttcacc
    37021 aaaatatcca tcatcacagg atcgagcaca ctttttggag cattgctctc aagcatatct
    37081 acatcggcca cttcctgatc ataatcatga tttaatgaaa ccatgataga atgaggcttt
    37141 ttgataaagt ttcttccctt atcggtcaat tttatcaagc catattgttc aatgtcttta
    37201 tacaccaatt cactcaacaa acattgacga ataatggaat tccagaattg atcgtctttt
    37261 tcttttccta cgccaaataa ttcaatttta tcgtgacgat aattttggtt ttctgttgtg
    37321 cgttttccgg tgaggaggtc aactacatat ttgatatgat gattttcgtt aatcacatca
    37381 attgttttca aagcattcac aacaaattct ttgccttcaa ttttctcttt tggttttaag
    37441 cagttgtcgc agctaccaca attctcttgt ttgtattctt cgccgaaata gaagagtaaa
    37501 aatttccttc ggcacacact cgtttctgca taagccgcag tttccgccaa tagctgtgca
    37561 cccatttcgc gttcggaaag tggcttgtcg cgcatgaatt tttcgagttt ctcgatatcc
    37621 ttatagctat agaatgcaat acattttcct tccagtccgt cacgacctgc gcgtccggtt
    37681 tcctgataat agttttcgat agatttagga atatcgaagt ggatcacata acgcacgtcc
    37741 ggtttatcga tacccattcc gaatgcgata gtagcaacaa tcacctgata tccttccatg
    37801 aggaatttat cttgtacttc agagcgcaga gcagcatcta atcccgcgtg gtatgcaccg
    37861 gctttgatac cgttgatgtt taatatatta gcaatatctt cggttgattt gcgatttaag
    37921 caatatacga ttgccgattt tctgccttgc tccttaatgt attttacgat ctgtttgatc
    37981 gcctgttcac gagaacgttt cggacgaatt tcataataga gattcgggcg attgaagctg
    38041 gaaataaata cattcggttc ttccaggtcg aggtttttct tgatatcgct ttgcactttt
    38101 ggtgtagcgg ttgcagtaag tgctatgata ggaatatcat cattgatagc ttctatcatg
    38161 ctgtgtatct gacgatattc aggacgaaaa tcgtgtcccc attcagaaat acagtgtgct
    38221 tcatctacag caacgaaaga aacgcgtatg ccgcggaaaa attcgatatt ttcttctttt
    38281 cgtaatgttt caggagcaac gtagagcatt tttgttcttc cggcaacgat atcggccttc
    38341 acctttttaa tttgctggcg attgagagaa gaattgagaa aatgcgcaat gtcgttattg
    38401 ctgctatagc tacgcaccag gtccacctga tttttcatca aggcgatgag tggcgataca
    38461 atgattgcgg taccttccag catcatggca ggaagctgat agcacaatga ttttccgcct
    38521 cctgtaggca tgatcacaaa agtgtcctgg ccattgagga ggcttgtaat tatttcttcc
    38581 tgatttcctt tgaacgaatc aaatccaaaa tactcttgta acgcgtcttt taatgttaaa
    38641 tgagatacat ccatctgctt cacgtgtttt tattgactga cgattgggta tatttgcggg
    38701 cgatttagac aaagcatgga atatgctgcc taaaaaccgg caacgagaat cgccttttaa
    38761 atataacaaa ttttaattac tttttaccaa gtagaattat atcatccgcg tcaatgagta
    38821 atgtttatgt agtaatcatg gccggtggca tcgggagcag gttttggcct gaaagcaggg
    38881 tcaaaaaacc gaagcagttc ctggacatcc tcaacacagg agaaaccctg atccaaacca
    38941 cctataccag gtttttaaaa atagctgaca aagataacat ttttatcgtt acgaatgaac
    39001 agtatatttc gctggtaaac gagcaaattc cgggcctaaa tccgctcaat atccttgccg
    39061 agcctagtcg caagaacacg gctccctgca tagcctatgc ctgccatgtg atagcccagc
    39121 gcgatcctga ggcaacagta gtagtggcgc ccagcgacca cctgatgctt aaccctgata
    39181 aattcctgct gacagtgctg gaagccttgg agtacgccga ttcacacgat gttttgctca
    39241 cccttgggat caagcctacc cgccccgata cgggctatgg ttatatccag tatgaagagg
//
LOCUS       DAOQOU010000008        37350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_457_length_37350_cov_6.559673, whole genome shotgun sequence.
ACCESSION   DAOQOU010000008 DAOQOU010000000
VERSION     DAOQOU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 37350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37350
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_457_length_37350_cov_6.559673"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..638
                     /locus_tag="PLM90_01340"
     CDS             231..638
                     /locus_tag="PLM90_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88037.1"
                     /translation="MSLTEGTKAAPFKLKTPPLTSDYEMYFDVKDGKDVIVCVVGKTT
                     LLYDRRCLNDLHAMLKNHGDWMELGSADEQKPAKEGTVEAWGRSAQNPVGGWYGLKKG
                     LRGRFGMYIPPLMEKLGLAEVTHDPKGNKMRAK"
     gene            731..1645
                     /locus_tag="PLM90_01345"
     CDS             731..1645
                     /locus_tag="PLM90_01345"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HMZ88038.1"
                     /translation="MIKTIILLLIAVVAIPLAAFYLDDPLSELQREMLHTSVYMMLAV
                     AAYCFIVGELTHNNSQVDKLWSIIPIVYAGYFAYASDWEPRVTLMSMLVAIWGIRLTY
                     NFSRRGAYSWKFWSGEEDYRWQILRKEPMFNTRLKWMLFDLFFICLYQNTLILLFTLP
                     AVMVAGLNTPLNFWDYLLALLMISAIIFEFIADQQQWNYQNEKLKRINSGAPLEGIYK
                     DGFVRSGLWKYVRHPNYAAEQLIWILFYCFSVAATGEWLNWSAAGFVLLLLLFQGSAD
                     FSEKISSSKYPAYAEYKKTVGRFLPKIK"
     gene            complement(1650..2171)
                     /locus_tag="PLM90_01350"
     CDS             complement(1650..2171)
                     /locus_tag="PLM90_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88039.1"
                     /translation="MMHFIENHTPIFETQVIIDKLAGINDDLHSAGFHFKVDVSDEIG
                     YTSYPNEEIPENTVIVLGSFDFTYYHQCEFAFHQVKAHKLPDYDWRDHWEKPQLILLR
                     GNDRIFALQSLSINDEPGLQVFAFNLGSFGDNCAYLACNAISIFVGTTFYYNRIKDTP
                     LEVGERMAWWVQR"
     gene            complement(2168..2761)
                     /locus_tag="PLM90_01355"
     CDS             complement(2168..2761)
                     /locus_tag="PLM90_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88040.1"
                     /translation="MQKDEIAGSYQLRGIMETAAGLELYPDGKFDFYYVYGAIDRHAY
                     GSWEISNEQIILNSLYTDKTGYDILLSEQRTQPRFTIYFPKADPFFISMQGAFAINGD
                     QAEEQKSDKKGWFLFQTGKAERIMLSNDLFPDRMITIENKDTSHNHIELAPNHDLLLI
                     HFSGVEAMITDDVLHLSSPFLKGMHGDRDFKFYKITQ"
     gene            complement(2769..4535)
                     /locus_tag="PLM90_01360"
     CDS             complement(2769..4535)
                     /locus_tag="PLM90_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ88041.1"
                     /translation="MKYLRICILTIALMGGRMSFAQSTIPADLLCAQTLVSGDVALTW
                     GASTEACGAFTEYQVYASTVFAGPYALLTTIPAIGTTNYTHVGANGTVTTWYYYIIAV
                     YACAGYTMTTSDTLDNLDPIAPELDYVTVTGGLSEIHWLPSPSAETNSYIIYRDIGGF
                     TPVGTVYGRFTTTFTDPTGTPTTGSETYTIAARDSCNNLGPFNNDAHHTIYLTAQQIN
                     CSDQLQLTWNEYDTWTAGVFEYQVWADRNFTGLSFIDNVPVGSPSYVLSGINDGDHIC
                     ITVRALRADGTATSISNEVCLDVNIVVPAAYNVMRNATVTGPTQIAIEWYPDAAADLE
                     KRSVQRSTDNVDYVNLITGPIVIPVPAIDSYVDNSIATNSIPFYYRTITTDSCGIETT
                     SGYVKTILLKGNDNANFTNDISWNDFEITNGTNIEYRIYRDDGAGFNLIATVTPGILT
                     YLDDVSAFVNVVDHFCYQVECLYQLNAPENGVNEQLTSLSNIICLDQGPRIYVPNAIV
                     IGGVNSLFMPVIIFGSSEGYLMQIFNRYGEVIFETTDINAGWDGSYKGSTVPMGTYGY
                     VITFTATNGQVITKKGNVTVLK"
     gene            complement(4620..5264)
                     /locus_tag="PLM90_01365"
     CDS             complement(4620..5264)
                     /locus_tag="PLM90_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88042.1"
                     /translation="MIITEEDIPYSSAEKFRKFLIKNYISQILLAFAVVTMVIAAIAG
                     VVMILAVAFFAAIMLALVSHLFLAITGRNLYSTVENETHGELQLPGNIQFKCIYEDKE
                     KLATIISTFQADASDFEDCDLVFISSDKAIQHIEERYFFRKYQTISDNIFLLEWHFDT
                     EHFSLGRIDLNASSYQTIIEVRRGVVPTLTVVDRSLIMRWNEPDFKKRITISGF"
     gene            complement(5390..6025)
                     /locus_tag="PLM90_01370"
     CDS             complement(5390..6025)
                     /locus_tag="PLM90_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88043.1"
                     /translation="MKKSLLLLAFTAYFLSARAQDTQYALKYDFFSPVAGCFGFSFEQ
                     PRNNFISFDYDLGLIGLRLDDYFTRDKFGGAYAAFGPRLYFDRDEAQTNDLRGAYFKP
                     QILVSYFQYQDNVDYYDYYSGLSYSGHLEGSDFSLSMLAAVGSQWIMSDLVVFDLWFG
                     LGYGGSWANETVDIPPGNYYDSGNNNFKYSHVHFGDSPLIFDGGLSIGFKW"
     gene            complement(6085..7383)
                     /locus_tag="PLM90_01375"
     CDS             complement(6085..7383)
                     /locus_tag="PLM90_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ88044.1"
                     /translation="MLSIQKKLSNSFYAILAMPATAMGFALCVQISALSWILSTKYDL
                     NIEEIGIVWASGPLAGLVMQLLVGLVSDNAWFWGGRRRPFIIIGGTIAALMLLCLPNL
                     DAIQKLFGTNSLIPVALAVATTLDLAINLGFNPTRSIITDVTPDGKARTKGYTWMQTV
                     SGTLGVAAYFIGAWWGNYQLIYVGVFIILIFSIIPVFFIEEPRVLQADTTDAEINALD
                     IQAHTQKAEVNELVKIYLANAFSWIGVQTMFVFMFAFASQMLFNTADTGSLNDEQAKR
                     TGQIISYSFLILNAVGAILPAFVLEPLTKKFGRITIQSTCLFVMALGYGLIVFFGKNA
                     GILYTCMAICGIGWAAIVSLPFAIMSEKVNKQRMGLFMGIFNMSIVIPQLIVSLLIAQ
                     FVDDAEDKKIIFEISAITLFISALIWRSVREKRANASSVK"
     gene            7466..8857
                     /locus_tag="PLM90_01380"
     CDS             7466..8857
                     /locus_tag="PLM90_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ88045.1"
                     /translation="MLKVLNCERNSDKKMEKPRLSFWQIWNMSFGFLGIQFGFALQNS
                     NASAILQNFGADIHQLSWYWLVAPCTGMIVQPLIGHFSDHTWTRLGRRKPYFLVGTIL
                     CCIALVLLPNSAAFVAGKAALMIGAGFLMIMDASINVAMEPFRALVADNLPDKQRTLG
                     FSIQTFLIGVGAVVGSWLPYVLANWYDVSSSAPEGQVADNVIFSFYIGAGVFFTAIMV
                     TILFAKEYPPAEYERYHGKPQKGKNFLADIFTDFSRMPKTMRQLGLVQFFSWFALFGM
                     WVFTTPAIAHHIYHLPIEDDSSDTFREASTWVGVIFGVYNLVSAIYAIFLPMIARALG
                     RKLTHALSLTCGGIGLVSIFFAKSPEMLIFSMIGVGIAWASILAMPYAILAGSIPAGK
                     MGVYMGIFNFFITIPQIINGIFGGPIVHTIYNDNAIYAIVMSGIFMFCAAVSVLYVHE
                     EGDITAIRTKPIS"
     gene            complement(8889..9980)
                     /locus_tag="PLM90_01385"
     CDS             complement(8889..9980)
                     /locus_tag="PLM90_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HMZ88046.1"
                     /translation="MSIDPNLPIRKRAVHFDLVRFIATFAVVQLHVATPWLWRESDTD
                     SYFWIVCIMFDTIGRAGVPIFLMISGALLLSHQEPIGNFFKKRFVRVFVPLIFWTVIY
                     EIRILADHHYLHGGVSADNIFEVLKNPFSGPVYDHLWFLYMIIGMYLVTPLLRKIVPV
                     LSRIDQWYLLGLWVFSDILLPLVYHYYDFSLGFDIPLVTPYLGFYLMGYYLMHHQFTA
                     KQIRIAWVVLISSYLLNVGMAWYDSHLHQGMQVFWLSHHRPGVFIMATSAFILLEHYA
                     MHAFSRFSEKKYKMLAYMGGTTFGIYLLHPLIIELFEGIFMGYIGIYPPFIHAVPFIA
                     IPVAAIIVTAVTMGIVTLMQRTPVLKKFV"
     gene            10175..10660
                     /locus_tag="PLM90_01390"
     CDS             10175..10660
                     /locus_tag="PLM90_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HMZ88047.1"
                     /translation="MRAPYLLLAISVSAIMVLSAGCSKSESPESAALAFFQALYNEHD
                     LEAAEALVTDNSREQLHNDFKLIEGTVAMLSENEPMRYEYKTLDEQTTIKGDSAFVTV
                     WTSLDSSTFETLLIKEDGDWKVDFSFAGQHLNAEKLVDQAIQQAMETTPKVLVENNLN
                     N"
     gene            complement(10757..11263)
                     /locus_tag="PLM90_01395"
     CDS             complement(10757..11263)
                     /locus_tag="PLM90_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88048.1"
                     /translation="MSISKNKVVSLTYELREDNISGDLIEVVKADQPFVFLFGAGGLL
                     PEFESNLAGKMIGSSFDFQIMAANAYGEFDESAMEYVPKDIFMIDGKLAEDLLETDKV
                     INLRDQDGHLVRARVAEVGEAEVLLDFNHPLAGVNLHFSGQILDIRDATAEELDHGHV
                     HGPGGHHH"
     gene            11426..13387
                     /locus_tag="PLM90_01400"
     CDS             11426..13387
                     /locus_tag="PLM90_01400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ88049.1"
                     /translation="MKSFTLKSMILLLGCMTFFTANAQTAGKDEVLFTVAGQPVTKEE
                     FLYVYKKNNPDKQNDYSSESLREYLDLYVNFKLKVTEARAEKIDTTEKVREELQKYGD
                     QLIKSNFDKEVLEGAAQRMYDRMQSERLVYHIMIGIDPKANGADTAAAIAKLTAARER
                     LLKGEDFGKVAAEVSTDKSSPDNPGFVGWVVPGQINDLTFENTVFSTAPGNISNVIKS
                     RYGYHIVKVAKERSSMGTVTVEHILVRVPDNAKPEDFAKAKVRIDSIAAQIKAGNGSF
                     EEMAAKYSDDKMTAQNGGKLESFGTGKLANDFQEAAFALKNPGDISAPVKTSKGWHLI
                     KLIERKPMGTYAEMKDELKGKIERSPEYKELRADFVSKAKAKYGFTENADAKKEMYAI
                     MDSSFVKNSWTMNKAGNMNKPMFTIGDKSMSQADFASFLEINQRSSRDKSIEEKFSKL
                     YTQAVEQTVIEYDLSERNIDFRRLLQEYRDGLPLFAMLERKIWSKGSTDSVGLKNYYE
                     QHKTEYMWEERIDASIYTCKDTKTAKAVRKMASKHASTESILAKFNKDSLELVTVKSG
                     LYMTGQDSNIDKMNKQTGIGEDILNADGTITFVQINKIVPPTPKTLDEARGYVISAYQ
                     DQLEKEWIAELHKKYPVAINEKVFNSMVH"
     gene            13512..14252
                     /locus_tag="PLM90_01405"
     CDS             13512..14252
                     /locus_tag="PLM90_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88050.1"
                     /translation="MSDIAGLVSDNTSPEDSARIVDDYIDNWIRQNLILRVAEDNLQT
                     AMAKINQQADEYKESLVLYAYERQFLAENLDTVVAEDSLRSYYDLHKQDFTLQGDIYR
                     LAYAVVSKDDKTADSVSYWLNRGLEKSRLALERYCVNHADRFSLNTDIWLNENDLFTL
                     LPYDMYAGGRFRSKAPIAFSDTVNRYFAMVADFYVEGDTGPFAYFRNDIKDIIINKRK
                     KELLNDTYQRIYQEGLKRDNAELLKKEE"
     gene            14249..15583
                     /locus_tag="PLM90_01410"
     CDS             14249..15583
                     /locus_tag="PLM90_01410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ88051.1"
                     /translation="MKKIVIVIAMLLPIAAFAQPVVDKIVAQVGDKIVLMSEVESYYY
                     DALQQGEVPDDYRCAILQELITQKLMLIQAEKDSIVVTDDEVSSNIDNRIRYFIQMFG
                     SEEKMEEFYGKSVQEIKEEFREDVKNMLLIDRMRESIVGGMSVSPTEVKEFYNSIPKD
                     SLPFVNAQIEYSHIVMVPKANAEQKQIAKEKADGILKRIKNGEDFCKLASLYSGDGSK
                     DNCGNLGCATRDTYVTEFSAAAFRLQPGEISDVVETQFGYHIIRMDSRQGDKACLSHI
                     LIMPPVTNLNFVAANKKLDSLRAQIMAKNITFCDAAKKYSDDEQSSKSCGVVINPQTG
                     SGTFEISELSPDDYYSIENLKPGEVSQVLPYTTADGKKAVRIIMLNDQTEPHQLNLKD
                     DYAKLQTFTLNVKQNKEMESWIRTKAEREYIRIDSIYSGCSSLNELMLANGK"
     gene            15612..16577
                     /locus_tag="PLM90_01415"
     CDS             15612..16577
                     /locus_tag="PLM90_01415"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ88052.1"
                     /translation="MATFNNEVEAIDALAADYKKLQAEIGKVIIGQDQVVKDVLLSIF
                     SDGHSLLVGVPGLAKTLLIKTISDALHLSFKRIQFTPDLMPSDITGAEILDDQRNFKF
                     VRGPIFANIILADEINRTPPKTQAALLEAMQEKHVTIGGHMHPLDKPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIVLDYPAYEAELQIVRATTSDNKVTLNKVIGGEEIMAYQH
                     VIRRIPVTDNVLNYAVGLVAKTRPNTEKADAKANQYLSWGAGPRASQFLVLGAKCHAA
                     INGKYAPDIEDVRAVALPVLRHRIVRNYKAEADGISVEDLIKGMM"
     gene            16701..18599
                     /locus_tag="PLM90_01420"
     CDS             16701..18599
                     /locus_tag="PLM90_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMZ88053.1"
                     /translation="MSDLIKHECGIALIRLLKPLSYYHEKYGTPLYGLNKLYLLMEKE
                     HNRGQDGAGIAAVKIDQKPGYKYFDRKRSISSSAIKDIFDEVFKKSHKMERKNPDLYH
                     NAEWAKQNVPFAGELLLGHLRYGTYGRNDIDSCHPFVRENNWITRTLLVAGNFNMVNN
                     DQLFQQLLELGQHPTQFVDTVTVMEKIGHFLDDEVQKLFDEKKEYHDNKTLSNIIGND
                     LDIQRILARAAKDFEGGYAMTGLFGHGDAFVLRDPNGIRPVYYYQDDEVVVAASERPA
                     IMTTFNVPFERVNELKPGHALIIKKKGSVKEAQCLQPRERKSCSFERIYFSRGNDKEI
                     YTERKELGRLLTDKVMKSVNYDFEHTIFSFIPNTAETAFYGMMKGLEQYLNNWKIAEI
                     KRLGSKITEEELHRILNMRPRVEKLAHKDVKMRTFITNDSDRSDLVSHVYDITYGQIN
                     DSSDSIVLIDDSIVRGTTLKQSIIAILDRLNPKKIIIVSSAPQIRYPDCYGIDMSKMG
                     DFVAFRAMTDLLKEQGKEQLIQDVYEECKTIQALPNNQTYNAVKKLYAQFSDEEISAR
                     IAKIVKADHIRAEVEVVYQSVEDLHKACPQNLGDWYFTGDYPTPGGNKVANKAFMNWV
                     EKKDVRAY"
     gene            18672..19916
                     /locus_tag="PLM90_01425"
     CDS             18672..19916
                     /locus_tag="PLM90_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ88054.1"
                     /translation="MNRTFLLACLIMLIAPLSAQLSFTGAGVLLQNETVNNYPVIKQV
                     LKFSPASDAGLQAGEVVWSVNGQSTEGKTIKDVVAMIKGNNGESRTLVVGSDRRTVDI
                     TIRLIKGNCTDGDCNNGEGRIEEPNGDIYVGNFTNGKFDGYGMYYYYTGEHMYARYDG
                     HFVAGKREGDGVLDNYDGGYKFTGTFKGGQANGNATMTFYNNNNTYTGNFIADKPSGS
                     GTLTFADGSTKTFSPTTVDEILLEAGVKQETQIADNTDNKSNNNNTDNNNNASDSYTS
                     GYDFSEMLNKMANVEYNLQYAMEKYASFYSAYRTTFLDNNATAAEEASVDELKACVEY
                     VEKAFNLLQAINNSSAIGESVTELQYEAMKQWSKAVNEAMELFVAGLAQDATVSTWYW
                     MNISSDKMEDLLDEASAARHAF"
     gene            complement(19946..20656)
                     /locus_tag="PLM90_01430"
     CDS             complement(19946..20656)
                     /locus_tag="PLM90_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88055.1"
                     /translation="MKKMLSLIALSLIVNNILFSQDTLILKLNNPAPRVGQSVDLSMQ
                     VNMVLDAMRSTMDSTITFESTFQGGEKFLTQTLVFSDTGKHILGPFKFDINGKSYITN
                     TLEINVAPELPLTEGIWIRVVENNGNQILIVEEQIKKSEPKSRISFGSKKSDFDYYGN
                     QNSFEKFAYLNSEPEGAAIEFNRYWMQTYDSSNLSFFTGLYNIYFLEDSGVVYTLGAK
                     DFINLPDNITVPTVVIAQ"
     gene            complement(20715..21047)
                     /locus_tag="PLM90_01435"
     CDS             complement(20715..21047)
                     /locus_tag="PLM90_01435"
                     /inference="COORDINATES: protein motif:HMM:NF023123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HMZ88056.1"
                     /translation="MKATITSLRLKSWVSFFPLSLYALRIMKQLKGSGHIEFKKTGFG
                     RMHYTMSLWKNEEDMRSFARSGAHLEAMRNSKKIAKEIRTITIDAESLPNWENAKKML
                     EQGKIIRF"
     gene            complement(21062..21439)
                     /locus_tag="PLM90_01440"
     CDS             complement(21062..21439)
                     /locus_tag="PLM90_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HMZ88057.1"
                     /translation="MQTIATTLTILVAIEHLYILYMEMFAWETLGRKTFKGALPDHMF
                     TPTKTLAANQGLYNGFLAAGLIWSLIISDNIWSSNVAIFFLSCVVCAGIYGAATASKK
                     ILITQALPAALALAACLAARCMN"
     gene            complement(21752..24067)
                     /locus_tag="PLM90_01445"
     CDS             complement(21752..24067)
                     /locus_tag="PLM90_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88058.1"
                     /translation="MPTNFHIAPPPKTVDGLFAVPMDIQSINGSITFNAATSVALADI
                     TIDYIVGPTAGRPFFDLRQNISSAILDGAAFPVAQLASHNFDTGTYTTLRVIDDVQAA
                     LSYHTLQLQYTLGTPQSQLGGSYIPVLDWLAGPSLKFVFGLSDLNAARYLEAWIPANL
                     PFDQYSIDLDIIITNTLVPHTVITNATVTTVGTNHWQLSFPSRFSCVSPLLEIRATST
                     VESQTGNTVLPVSGKNITIEAFKLVGNAANLNTEINNIATFLAENEEAYGAYLHDNKF
                     TALFTTINGGMEYEGGTTTASWALNHETFHSWFARGMKPAAQCDGWWDEAFTTFHDDG
                     ANDTLPFDYSDAPITLCSRNPWQRTTPGNSYDDGGRFWQGIAAILGVNTTNNLMRDMH
                     NTYKGKSPVSTQMMEEFLICKSGNKDLVDAFHRFIYGFDDIGSPDLWMKDHTSHTGSD
                     QWDGAFWDSPDLWIRNSDDDGTTHQSPEYGQDNWFYARVRNKSATNEAQHFVVTFNHK
                     SWAGTQFLFPNDFLPAVAAKAEFTLGPGETRIVKAKWPRANIPAVGSHACVLASILCR
                     GDHPVNNQHVWEHNNLAQKNTTVVDLLPNAFIIIPVVLTNYFDLLSDYIIEAWRDRRM
                     EGYEVSLLHKDKMFFEGNDLKTIIHPDFSLKDHFKFDDRHALMDCGGHVDHYTQTIGM
                     ITDQRPDLLIRKLGNALEMPFSKEKVSGLSTNIPYQSQRVVGFKVKVPESAKPGTTFT
                     THLVQRHAKTKTITGGIAVTIHVADGRKTEK"
     gene            complement(24132..27077)
                     /locus_tag="PLM90_01450"
     CDS             complement(24132..27077)
                     /locus_tag="PLM90_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88059.1"
                     /translation="MNSKYESPLKAFVTRDKKRIARQVLHIQEPALSNQKIPRSAAFD
                     YIRNMADDFKISESHLQNMQKPLAYNTPIEQDMELRIVDEKRYFDSNTIRMAQTINNI
                     PIWRKGLTVTVKQNPNRIVYAANNTVASFRIKLASEKAISRYQSMFASIAHKPIVPLL
                     SEEGQKDDPQTGDFVRGFAMNIKANAKTDRQAGKRNPARLIRGRMYYYAYDENKRQAV
                     HSHNITKDKVRVGEEHEHPSFPLPPIDKSIKNGQYYLVAEITFAWKIANEMQTWLALV
                     EMETGSILYIEPLFAGVSGKVFVQDPITSSGDTTQTANLNNATLNPYRTSVTLNNLDA
                     PVGGVQSLTGSNVQLVDVDGRTEPPPTKAVGDDFDYNVRTNDFAAVSAYYHTDNFFET
                     IESLGFDLSTYFTNTSFPVDIDHADQIDVNAHCMGNGVDGIDHSGYGYGDETNMVDPT
                     IGRAPDNWVHWHELGGHGILYDHVGTWYFNFAHSAGDGLAAIQNDPTSQLRELGNIER
                     FRYAPFRYPALDRWFNRDVAAGWGWDGSNDIGGYESEQILATSNFRWYRSIGGDSSSL
                     SRRQFASRMATYLILRAVSILTPETNPAHAQGFCDALMAVDLLDWTSEGISGGAYNKV
                     IRWAFEKQGLFQPAGAPMPVTTAGAPPEVDVYINDGRNGEYEYQAVHWHNISMWNRQH
                     DDGGLTHEDAIPDATNYMYVKVKNRGTSNSGNVNVRGYHCLPGAGLTWPTDFTEMGPG
                     GGLNVASIAANNAEEVIVGPFEWVPNENIYGHDCTLMIVSTDGDPSNVDNFTLGDTVA
                     EWRLVPNDNNIGQRNVTILPGGGGENALIASLHDHFFWAGNPFRVQTKMQLKVELPKF
                     LAEAGWKVQFKDSKDQFTLKPGEKRKMVIDVIKGRDFSTEQVHAAYQRDIVIGLYAED
                     MLIGGMTYRIDPDKKEPGYIPGKKDRCTDKAQDLLDCLHLNSGKVKKVCIKKVSLDIE
                     LNSDNCQCD"
     gene            complement(27384..27887)
                     /locus_tag="PLM90_01455"
     CDS             complement(27384..27887)
                     /locus_tag="PLM90_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ88060.1"
                     /translation="MPIIRLTTTIHCDIEVAFDLSRSIDLHMMSTKSTNEVAVAGVTS
                     GLISLNESVTWEATHLGIRQQLTAKITIFERPFHFRDEMVKGAFDYFKHDHFFEAQSH
                     TTIMTDVFEFTSPFGVVGKLFDRIFLTRYMTKFLVERNNTIKQIAESPDSYKFLHFDK
                     NGVHGKQ"
     gene            complement(27954..28475)
                     /locus_tag="PLM90_01460"
     CDS             complement(27954..28475)
                     /locus_tag="PLM90_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ88061.1"
                     /translation="MSFSIERSIEILERTPLVLKQLLSGLPEHWTGVNEGPETWSPYD
                     VIGHLIHGETTDWTKRMDIILSDGGNKEFATFDRFAQFQESKGKSLEQLLNTFHDERK
                     SNITYLLSKNISKTDLQKTGIHPKFGKVTLQQLLSTWVAHDLDHIMQISRVMAKQYRE
                     DVGPWKEYLKIMQ"
     gene            complement(28491..28880)
                     /locus_tag="PLM90_01465"
     CDS             complement(28491..28880)
                     /locus_tag="PLM90_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ88062.1"
                     /translation="MHPDINIYHEALTESDKAIADTLASLIDKHLPNAESKIWHAHPV
                     WFIDGNPIVGYSKLKDSVRLLFWSGQSFEEPDLLPEGKFMAAEARFTNVNQIQKSDLK
                     RWLNKAAEIQWDYKNIVKRKGKLERLK"
     gene            complement(29048..29608)
                     /locus_tag="PLM90_01470"
     CDS             complement(29048..29608)
                     /locus_tag="PLM90_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HMZ88063.1"
                     /translation="MKTNKIAIVAFVLLAALQIYLPASWAIESAETLSYGKVLKFRTI
                     PVDPKDLFRGNYIALNFENTRITVSDADDWKEGDNIYIVLNVDEGQFATYSHYEKDKP
                     RPDINFVTATVKHISRSPEPYVEIEYPFSKFYVEESIAKAAETAYRNAIAEGHNTYAV
                     VMAENGKTVLKDVYIDSKSLTDGLKD"
     gene            complement(29595..30878)
                     /locus_tag="PLM90_01475"
     CDS             complement(29595..30878)
                     /locus_tag="PLM90_01475"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HMZ88064.1"
                     /translation="MLKDLHELVEAGILTEETAQQVSEYYDKKASTKGNRRLFVAFGI
                     LGAVLVGSGLILFIEHNWDLFSHLQKTIFAFIPLVLGQILCGTLLFRNRLESLWKEAA
                     SVFLFFSVGACIALISQIYHLKYSEEKILLIWMLLSIPIVYIMRSPMSSLLYIAGITW
                     YGGKYAFDYHEGFSRLYWLLIAMALPYYINLLQRKSINNFTSLHNWFIPISMSLCLTL
                     LTQDYDEALILMYFGYFSLIFAIGNSSLFSAVNTGKNGFKIIGFLGMLVILFISSNFI
                     AFDRGFYKSIHRFNFVKDDGFIFALALIITSGIVTFLNHRWAGKSKLALWEYTGFIFL
                     ATYVLNEFTVVAVILYNIYIFAAGITFIFLGSKRNHLGLMNLGLSIIAVLAIYKFFES
                     DLSYQLKGVLFMLVGAAFVTFNVIMLKRRRIYENQ"
     gene            complement(31291..34308)
                     /locus_tag="PLM90_01480"
     CDS             complement(31291..34308)
                     /locus_tag="PLM90_01480"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ88065.1"
                     /translation="MLSRFLLLLLLLGAVSRGYLFSQIIINEGSNKSYLTGIDEDGEG
                     SDWIELYNNSDVPADLSGYFLSDKVSEPTLWSLTDFTLEPHAFTTIYCSEKDRHHTTP
                     FTLAIDETDFIPEVGWNTHYFTTAYDWDGVSNLIFNICSYNNTGYTENSVFQQTATPY
                     ASTSATFVDGSDASCSNVLGTLYFQRPNLQINGITIDFGSILNSNVDYPAPYGNWYWS
                     ARHQILVRAEELIAAGLSAGPISSLAFQVASTNVITYTYIDIAINSTSEDALSESMIP
                     VNGYADHTNFKIDADGENIYLNDATGSLVSSLMVNSPIADISVGRSPDGTEDIVWMTP
                     SPGMTNNAANVFTDTLISPVLSVPTSIVDDAFNLIITNPNTGAVATRVVYTLDGSEPT
                     FTSTIYTGTPIPINENIVVRARVFPETDATYLPSYDAIATYLFGVNHSTPILLVTTDN
                     INLYGATGIFDNYNSDWIKAAHAAYLSKEIGHPILFETRTAMRMDGGAGGSRANPQHS
                     FRLSFDHAALGEKTIHEQLIPAIPFRNDYSDVYLRNGSNQWLTLPYKDACQVSLMSNG
                     TNNYYSAMEPVSVYINGSYFGLYELREKFNTEYFDEREDINEDSTEILSLSYFYNLLL
                     RALEGDVDNFYADYNAFNALNPMDTDYIDQADIYFDLEHYTDYIIGESWMGNTDWPYN
                     NIKIYRSDKTNWRWRFALIDLELSLMPNSWTNCSYNHIAYMLSQGTDNPYINIWLQSI
                     QNETYLNQFINRFADLMNTAYLIDTLLAGEQAIYDKWAPEMPNEYARWGDPLNVEGQM
                     ADFTARHETFRQQLECRSDKVRGDLLSEFDLTKEVTVHLDVYPELAGTIHLNTIEPGV
                     YPWTGIYFDGVPITLTAQADSGYIFSYWEPSAFIIDTLNAHFTSNISDAEVTFTAVFK
                     QPEEPDGTTIQFNVYPSPSNGSITIAHNNATLAKDATYSIYNETGQFVMNGNLSESAL
                     TTTLNVTHLAPAIYYVRINRGGDILQSLSFVRL"
     gene            complement(34561..35244)
                     /locus_tag="PLM90_01485"
     CDS             complement(34561..35244)
                     /locus_tag="PLM90_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88066.1"
                     /translation="MKKTIIISLFISYSGFAFCQSRPDSLQHNQIGLDVAYGIRSYLN
                     FNEMTWPDAPEYLMIYRRLFDKGSLRIAGNVSFTLENPKSYYDEDSNHYQNSETFLQL
                     SVGYEWFKMLDKRWQIYYGLDGIFNVRSIDFDLNYYNAGYANGTESSLVKYGLAPLLG
                     LRLYCTPRISLTTETSILIAIEEFQSTRTYIPISSDYPAIENVAEPSLTTIHNIYTPS
                     ISLTLNISL"
     gene            complement(35264..35956)
                     /locus_tag="PLM90_01490"
     CDS             complement(35264..35956)
                     /locus_tag="PLM90_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88067.1"
                     /translation="MKSTLSGFLLLMISRFALSQNAFTDDPMLHKNQLGINASYFVSQ
                     FINFDGFDYYQYPSYMLYYRRILAPGNIRVAFGANYNSEEIVSRYDYDSTLYTNIDYD
                     VAFSAGWEWDTPISKKWWVYYGADIGTFMQFENYEADNFDDGYAISTKTKDLRHSIGP
                     FIGVRFDLTPRISLTTETNLKINFNTVDVHRTYTPTSSDYPELPDETDPTQKSLYTGY
                     EVPYTLVLTIDL"
     gene            36139..37107
                     /locus_tag="PLM90_01495"
     CDS             36139..37107
                     /locus_tag="PLM90_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88068.1"
                     /translation="MPIHYKYLIGSIAACICYVHCYTQNYVPLVVEGNTWFEEYEYMS
                     AYGDLTSFLIDQDTIIDDMQYWRLRKWVQHSSAGWMGQVEINPVYEDTIPIYFLREDT
                     VERKVYLREQWPLITNEVLMYNFNLEVGDTLDFGDTSEVKIVTSIDSVLMNTKWHKRM
                     HITPEYAPVENYVDLIEGIGSTFGLNQGVEFPFEESMRLLCFSNDDSLYINFYVDEIP
                     ISVDSAEICDFLPSNVSIDGLQQQLNFDVYPNPVGQIMHIDLHASLVGVNDILLFNSI
                     GELSKHLLHIDSASLEINVSDLLPGMYVLVVRGINAAAISTTVIIQ"
     gene            complement(37164..>37350)
                     /locus_tag="PLM90_01500"
     CDS             complement(37164..>37350)
                     /locus_tag="PLM90_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88069.1"
                     /translation="GAAAYDVLGQLGILTEYDHQKTYRKIFYPKKLRKPLLEKAEQNN
                     WTIEKHAGSVTRRWEKD"
BASE COUNT        11211 a         7385 c         7729 g        11025 t
ORIGIN      
        1 agatgccaag gtgtttaata ttagggtgcg gaatgcatat gtttcgcacc ctaatgtttg
       61 tttttgcaat tgtccaaata taattttgat tcagggacgc ttgacaagtc aatttgaatt
      121 ttaaggcgat gttcttcaag gcgcattgca gattcgcata catgggatta tttgttgatt
      181 aaattaaata ttaatcttta gtttagtaat tttatacaaa ttcaaatttg atgtcattga
      241 cagaaggcac aaaagccgcc ccattcaaat taaaaacccc gccgcttaca tcagactatg
      301 aaatgtattt tgatgttaaa gatggtaagg acgtgatcgt atgtgttgtg ggcaaaacca
      361 ctttgctata tgatcgccgt tgcctgaatg acctgcatgc gatgctgaaa aaccatggcg
      421 actggatgga gctgggaagc gccgatgagc agaagcctgc aaaggagggc actgtagaag
      481 cctggggtcg ctccgctcaa aatccggtgg ggggatggta tgggttgaaa aaaggacttc
      541 gcggccgctt cggtatgtat attccgccac tcatggagaa acttgggctc gccgaagtta
      601 cacatgatcc aaaggggaat aagatgcgcg caaagtgagg cagatttcgc tcagcataaa
      661 attgcatttg gtatgttgat actcagttat tagatacaga aaaattcatt ttctttatca
      721 gtcattactt atgataaaga cgattatcct tttgttgatt gccgttgtgg caattccctt
      781 ggcagccttt tatctcgacg atccgctcag cgagttacag cgggaaatgt tgcacacgag
      841 tgtttatatg atgctggctg ttgccgctta ttgttttatt gtaggcgagt tgacacacaa
      901 taatagtcag gtagataaat tgtggagcat cattcccatc gtttatgcag ggtattttgc
      961 atatgcttca gactgggagc ctcgtgtaac actgatgagt atgctggttg ctatttgggg
     1021 tattcgtctg acatacaatt ttagtcgccg aggtgcctac agctggaaat tttggagtgg
     1081 ggaagaagat tatcgctggc agatattgcg caaagaaccc atgttcaata cgcgattaaa
     1141 atggatgctg ttcgatttgt tttttatctg tctttatcaa aacacactta ttctattgtt
     1201 tactctcccg gctgttatgg tggcgggatt aaatacgccg ctaaattttt gggattattt
     1261 gttggcattg ttaatgatat cggcaataat attcgaattc attgctgatc aacaacaatg
     1321 gaattatcaa aatgaaaaac taaaaagaat taattcaggt gccccgcttg aaggaatata
     1381 taaggatgga tttgttcggt cgggattatg gaaatatgtg cgccatccca attatgccgc
     1441 tgagcagttg atctggattt tgttctattg tttttcagta gccgcaacag gtgagtggtt
     1501 gaactggagc gcagccggct ttgtattgtt attgctgcta tttcagggaa gtgcagattt
     1561 cagcgaaaaa atatcttcat caaaatatcc tgcatacgcc gaatacaaga aaactgtagg
     1621 caggttttta cccaaaataa aataataaat taacgctgca cccaccacgc catgcgctca
     1681 ccaacttcta acggcgtgtc ttttatccga ttgtaataaa atgtagtgcc aacaaatatg
     1741 ctgatggcgt tgcacgctaa ataagcgcaa ttatctccga aggaaccaag attaaatgca
     1801 aaaacttgta atccgggttc atcattaatg gaaagcgact gcaacgcaaa aattctgtcg
     1861 ttaccgcgga gtaatattaa ttgaggtttt tcccaatgat cgcgccaatc ataatccgga
     1921 agcttgtgcg ctttaacttg atggaatgca aattcacatt gatgataata ggtaaaatca
     1981 aagctaccta agacaatgac tgtattttca ggaatttctt cattcgggta ggaggtgtat
     2041 ccgatttcat ccgaaacgtc aaccttaaaa tgaaatcctg cactatgtaa atcatcgttg
     2101 atccctgcaa gtttgtctat gataacttgt gtttcaaaga ttggagtatg attctcaata
     2161 aaatgcatca ttgcgtgatt ttgtaaaatt taaaatcacg atcaccatgc atgcctttta
     2221 gaaatggtga actcaagtgc aatacatcat ctgtaatcat tgcctccaca ccgctaaaat
     2281 gaataagcag caggtcgtga tttggggcaa gctcaatgtg attatgtgat gtatccttgt
     2341 tttcaatagt aatcattctg tcgggaaaga gatcattact caacatgatt ctttcagctt
     2401 tgcctgtttg aaataaaaac caaccctttt tgtcggattt ttgttcttca gcctgatcac
     2461 catttattgc aaatgcgccc tgcatgctta tgaaaaatgg atctgctttc ggaaaatata
     2521 tagtaaaccg tggctgtgta cgttgttctg aaagtaatat gtcgtaaccg gttttatctg
     2581 tatacagaga gttcaaaatt atttgctcat tgctgatctc ccaactgcca tatgcatgtc
     2641 tgtcgatggc gccatacaca taataaaagt caaacttacc atcgggatac aattccaatc
     2701 ccgcagccgt ttccataatc cctctcaatt gatatgaacc tgcaatttcg tccttttgca
     2761 ttcgtatgtt atttcaatac ggtaacattc ccttttttgg tgatcacctg tccattggta
     2821 gccgtaaatg tgatcacata accgtaagtt cccataggta cagtgctgcc cttgtaagaa
     2881 ccgtcccaac cggcattgat atcggtagtt tcaaaaatta cttcaccata gcgattaaat
     2941 atttgcatga gatatccctc cgaagaaccg aaaatgatta caggcatgaa taaggaattc
     3001 acaccaccta taacaattgc attcggcaca taaatacggg gaccctgatc aaggcagatt
     3061 atattggata aggaagtgag ttgctcattt acgccgttct caggtgcgtt taattgatag
     3121 agacattcaa cctgatagca gaaatgatca accacattta caaatgcaga tacatcatcg
     3181 agataggtta aaattccggg tgttactgtt gcaatgagat taaaccctgc accatcatct
     3241 ctgtaaatgc gatattctat attagtgcca ttggtaatct caaaatcatt ccagctgata
     3301 tcattggtga aattagcgtt atcatttccc ttcagcaaaa tcgtcttcac atatcctgat
     3361 gtagtttcta ttccacaact gtcagtagta atcgtgcgat aataaaatgg aatagaattg
     3421 gttgctatgc tattgtctac ataactgtca atggccggta cgggaattac aatcggtcct
     3481 gtgatcagat tgacataatc aacattatca gtgctgcgct gaactgatct tttttccaga
     3541 tcggcagcgg catcgggata ccattcaata gcaatttgtg ttggcccggt aactgtagca
     3601 ttgcgcatta cattgtaggc agcggggaca acaatattta catcaagaca aacttcatta
     3661 gaaatggaag tggcagttcc atctgcacga agtgctctca ccgttatgca gatatgatcg
     3721 ccatcattga ttccggaaag tacatacgat ggagaaccca ccggaacatt gtctataaaa
     3781 ctcaaaccgg taaaattcct atcagcccaa acctgatatt caaatacacc ggctgtccag
     3841 gtatcatatt cattccacgt taattgcaac tgatcggaac aattgatttg ttgcgctgta
     3901 agataaatag tgtgatgtgc atcattgtta aaaggaccta aattattaca tgaatcgcgt
     3961 gcagctatgg tatacgtttc agatcctgtt gtaggtgttc cggtaggatc tgtgaatgtt
     4021 gtagtaaatc gaccgtatac agtacctaca ggtgtaaacc ctccgatatc acgataaatg
     4081 atatatgaat tagtttctgc cgaaggtgat ggcaaccagt gtatttcaga taagcctcct
     4141 gtaacggtaa cgtaatccaa ttcaggagca atcgggtcga gattatctaa cgtgtcgctg
     4201 gtggtcatgg tataaccggc acaagcatat acagcaatga tataataata ccaggtggta
     4261 acagttccgt tagcaccaac atgcgtataa ttcgttgtgc caatggcggg aatagttgtg
     4321 agaagcgcat agggccccgc aaaaacggtt gaagcataaa cctgatactc cgtgaatgca
     4381 ccacatgctt cggtagacgc accccaggta agcgctacgt cgccactcac aagtgtttgc
     4441 gcacagagaa gatcggcagg tatggtcgac tgtgcaaatg acattctacc gcccatcaaa
     4501 gcaattgtta atatgcaaat acgcaaatac ttcactgtcg cagattggtc tttaactata
     4561 acgcaaaatt gccataaata tttgtaatgc ctgctatctg tgcaacaagg gttctgtgct
     4621 tagaagccgg atatggtgat gcgcttttta aaatcgggct cattccaacg catgatcagg
     4681 ctgcgatcaa caactgtcag ggttggcact acacctcgtc tgacctctat tatcgtttga
     4741 taacttgagg cattcaagtc tattcgtccc agtgaaaagt gttcggtatc aaagtgccat
     4801 tccagcagga aaatattatc acttatcgtt tgatactttc ggaaaaaata tcgctcttct
     4861 atatgctgta tggccttatc ggaacttatg aaaacaaggt cgcaatcttc aaaatcagag
     4921 gcatctgcct gaaatgttga gattattgtt gcaagctttt ccttgtcttc ataaatacat
     4981 ttgaattgaa tgtttcccgg aagctgtaac tcgccgtgtg tttcgttttc aactgtagaa
     5041 tatagattcc ttccggtaat agctaaaaat aaatgcgaaa ccaaagctag cataattgcc
     5101 gcaaaaaatg ccaccgccaa tatcatcacc actccggcaa tagctgctat taccatagta
     5161 acgactgcaa aggcaagtaa tatctgactg atataattct tgatgagaaa tttcctgaat
     5221 ttctccgcac ttgaatacgg aatgtcctct tcggtgataa tcaaatttag acaggcttaa
     5281 aaaataattt aggcaaaatt ataatgctca aatgataaat agattaaaat atcagtgtgc
     5341 actaaatgta atcggcaaac acataaaatg ccgcccgatg aagtataaat taccacttaa
     5401 acccgataga taatccgccg tcaaagatca atggtgaatc tccaaaatgc acgtgcgaat
     5461 atttaaaatt gttatttccg ctatcgtagt aattacccgg tggaatgtca accgtttcat
     5521 ttgcccaact gccgccatat cccaatccaa accaaagatc gaatacaaca agatcgctca
     5581 tgatccactg tgaacctacg gccgcaagca tactcaatga aaagtcgctt ccttccagat
     5641 gacctgaata ggaaagaccg gaataataat cgtagtaatc tacattatct tgatattgaa
     5701 agtagcttac aagaatctgg ggtttgaaat aagcacctct aagatcattg gtctgtgctt
     5761 cgtcgcgatc aaagtataac cttggtccaa aggccgcata agcgccacca aatttatcgc
     5821 gggtaaaata atcgtcgagt cttagtccaa ttagcccaag atcatagtcg aaggaaatga
     5881 aattatttct cggttgctca aaggaaaatc caaaacaacc ggcaactgga gaaaagaaat
     5941 catatttcaa tgcatattgc gtgtcctgag ctcgggcgga aagaaaatat gcggtgaatg
     6001 caagtagcag taggctcttt ttcattttag ggctttttgt aaagatagtt aattcacttg
     6061 atttgtaaaa ggggggatgt cacctcattt taccgaagat gcattcgccc ttttttcgcg
     6121 caccgaccgc cagatcagcg cactgatgaa caatgtaatg gcggatattt caaatatgat
     6181 ctttttatcc tcggcatcat caacaaattg agcaattaag aggcttacga tcaattgtgg
     6241 aattacaata ctcatgttga aaatacccat aaacaaaccc atgcgttgtt tattcacttt
     6301 ttcactcatg atagcaaacg gcagacttac gatggcagcc caaccaattc cgcatatggc
     6361 catacaggtg taaagtatgc ctgcattttt accgaagaaa acaattaatc catagccaag
     6421 tgccatcaca aacaaacaag tagattgaat ggtaatgcga ccgaattttt ttgtcagcgg
     6481 ctctaataca aatgcaggta gtattgcgcc tacagcattt aaaatgagaa aactataaga
     6541 aatgatttgt ccggttcttt ttgcctgctc atcatttaat gatccggtat ccgctgtgtt
     6601 aaacaacatc tgcgaggcaa aagcaaacat aaatacaaac atggtttgca cgcctatcca
     6661 tgaaaatgca tttgccaaat atattttcac caattcattt acttccgctt tttgtgtatg
     6721 agcctgaatg tcgagcgcat taatttctgc atcggtggtg tcggcctgta aaactcgagg
     6781 ctcttcaata aaaaataccg gaatgatgct gaaaattaag atgataaaca cacccacata
     6841 aatcaattga taatttcccc accaggcacc aataaaatat gcggcaacac caagcgttcc
     6901 ggaaacggtc tgcatccagg tataaccttt agtgcgcgcc ttgccatcgg gagttacatc
     6961 agtgatgata cttcttgtcg gattgaagcc aagattaatg gcgagatcta gtgtagttgc
     7021 tacagccagt gccactggaa taaggctgtt tgtgccgaat aatttttgaa tggcatcaag
     7081 gttgggcaag cacaatagca tcaaagctgc aatggtgcct cctataataa taaatggcct
     7141 gcgtctgcct ccccaaaacc aggcgttatc gctgaccaat ccaaccagca attgcatcac
     7201 caaccccgcc aatggtccac tggcccatac aatgcctatt tcttcaatgt taaggtcgta
     7261 cttggtcgat aagatccaac tcaaagcact gatttgcaca caaagcgcaa aacccatggc
     7321 tgttgccggc atggccaata tggcgtaaaa tgaattgcta agttttttct gaatggaaag
     7381 catatttcgc tatttatgat tgctgaatat cacccaataa atataaaccc cgaaatacct
     7441 gtatggatac agacttggaa gtgacgtgct aaaagtatta aattgcgagc gaaatagcga
     7501 taaaaaaatg gaaaaaccac gattgagttt ttggcagata tggaatatga gctttggatt
     7561 cctggggatc caatttggct tcgccttgca aaacagcaac gcctctgcaa ttctccaaaa
     7621 ctttggtgcc gatatacatc aattgagctg gtactggtta gtggcaccat gcacaggtat
     7681 gatcgttcaa cctttaatag gtcatttcag cgatcacaca tggacaagac ttgggcgaag
     7741 aaaaccttac tttttagtag gcacaatatt gtgttgtatc gctttggtat tacttcccaa
     7801 ctcggcggcc ttcgttgcag gtaaagctgc actgatgata ggtgccggtt ttctgatgat
     7861 catggacgcc agtatcaatg tggccatgga accttttcgc gcccttgttg ccgacaacct
     7921 tcctgataaa caacgcacgc ttggttttag tattcaaaca ttcttgattg gtgttggcgc
     7981 agttgtagga agctggttgc catatgtgtt agcgaattgg tatgatgtct cttcttctgc
     8041 tcccgaaggt caggtagctg ataatgttat cttttcattt tatatcggtg ccggcgtatt
     8101 ttttactgcc atcatggtaa cgatattatt tgcaaaggaa tatccaccag cagaatatga
     8161 acgctatcac ggtaaacctc aaaaagggaa gaatttctta gcagacatat tcactgactt
     8221 ttcgcgtatg ccgaaaacga tgcgccagtt gggattggtg cagtttttta gctggtttgc
     8281 tttgttcggt atgtgggtgt ttacaacacc tgcaatagca catcatattt accatctgcc
     8341 aattgaagat gattcaagtg atacattccg cgaagcaagt acatgggtag gtgtgatatt
     8401 tggcgtgtat aatttagtaa gtgccattta tgccatattc cttcctatga ttgccagggc
     8461 attaggtaga aaactcacac acgcactatc gctcacatgc ggaggtatag gtttggtttc
     8521 catcttcttc gcaaaatcac ctgaaatgct cattttttct atgataggtg taggtatcgc
     8581 atgggcaagt attcttgcaa tgccatacgc catactcgca ggaagtatac ccgcaggtaa
     8641 aatgggcgtt tatatgggaa tatttaattt cttcatcacc ataccacaaa ttatcaatgg
     8701 catttttggc ggcccgattg tgcatactat ttataacgat aacgccattt atgctatcgt
     8761 aatgagtggt attttcatgt tctgcgcagc agtttccgta ctgtatgtac atgaggaagg
     8821 tgatattacc gccatccgca caaaaccaat cagctgataa attaatcgcc ggttttttta
     8881 cttgctgatc aaacaaattt tttcagcaca ggcgtccgct gcataagcgt tacaataccc
     8941 atagtaacgg ctgtaacgat gatcgcagct accggaatgg caatgaatgg cacggcatga
     9001 ataaatggcg gatagatgcc gatatatccc atgaatatgc cttcaaataa ttcaataatc
     9061 aacggatgta ataaataaat accgaaggta gtgccaccca tataggcaag cattttgtat
     9121 ttcttttcag agaagcgact aaatgcatgc atggcataat gctccaataa aataaacgct
     9181 gaagttgcca tgatgaaaac gccgggacgg tgatgcgata accagaatac ctgcataccc
     9241 tgatgcaaat gactatcgta ccaagccata ccaacattca gcagataact ggaaatcaaa
     9301 accacccatg caatgcgaat ttgcttggca gtaaattgat gatgcattaa ataatacccc
     9361 atcaaataaa atcccaaata tggcgttact aacggaatat caaaccccaa tgagaaatca
     9421 tagtaatgat atacaagtgg taaaagaata tcgctgaata cccataaccc caataaatac
     9481 cattggtcaa ttcgcgaaag caccggtact atcttgcgta acaatggcgt taccagatac
     9541 atgcctatga tcatatataa aaaccataag tgatcatata caggccctga aaatggattc
     9601 tttaatactt caaaaatatt atctgccgaa actccaccgt gtaaataatg atgatcggca
     9661 agaatgcgta tttcataaat gactgtccag aatatcagtg gtacaaaaac tctgacaaat
     9721 cgttttttga aaaagttgcc gatgggttcc tgatgactga gcaataaagc accgctgatc
     9781 attaaaaata tcggcacccc tgctcgtcca atggtatcga acatgatgca tacaatccaa
     9841 aaataactgt cggtatccga ctcgcgccat agccaaggtg tggccacgtg caattgaacc
     9901 accgcaaacg tggcaatgaa tcgcaccagg tcaaaatgca ccgcccgttt ccggatcggc
     9961 aggttcgggt caatactcat gcggcacaaa tttagtgttt tcaatcagag gaaaactgtc
    10021 gtcactaatt caatgaaatg tgcactttta agcaggtcat aaattcgata attctagatt
    10081 gccgggctca gattgttaaa ttctggtaaa atattgaatt caatacccaa tcctcacggc
    10141 gaacagttta ttttgtatct caacccaagt cccaatgcgt gctccctatt tgttgttggc
    10201 catcagtgta tccgcaatca tggtattaag cgccggttgc agtaaatccg aatctcccga
    10261 atcggcagcc cttgcctttt ttcaagcact ttacaatgaa cacgatctgg aagctgcaga
    10321 ggctttggtt actgataata gtcgcgagca actgcacaac gactttaaac tcattgaagg
    10381 cacagtggcc atgttgtctg aaaatgaacc aatgcgttat gaatacaaaa cactggatga
    10441 acaaactaca ataaaaggcg actcagcctt tgtaaccgta tggacttcgc tcgatagcag
    10501 caccttcgaa acactgctga taaaagaaga tggcgattgg aaggtggatt ttagctttgc
    10561 cggtcagcat cttaacgcag aaaagttggt agaccaagcc attcaacaag ctatggaaac
    10621 tacccctaaa gttcttgttg aaaataatct aaataattga ttgtcaatta tttgtgaaac
    10681 atccctgagc acaattatag ggatgtccat tcaacacatc agtgtacagc cgcctgtcca
    10741 gttaaataag caccaattaa tgatgatgac cgcccggacc atgcacatga ccatgatcca
    10801 gctcttcggc tgtggcatca cggatgtcga gaatctgacc ggagaaatgc aggtttaccc
    10861 ccgcaagcgg atggttgaag tccaataaaa cctcagcttc accaacctcg gcaacgcgag
    10921 ctctaaccaa atgtccatcc tgatcgcgca gattgatcac tttatcggtt tccagcaggt
    10981 cttcagccag ttttccgtcg atcataaaaa tgtctttcgg cacatattcc atggcagatt
    11041 catcgaattc gccgtaggca ttggctgcca tgatctggaa atcgaatgat gaacctatca
    11101 ttttgcccgc aaggttgctt tcaaattcag gcagaaggcc gcctgcacca aagaggaata
    11161 cgaaaggctg atctgccttc actacctcta taaggtcgcc gctgatattg tcttcacgca
    11221 gttcataagt cagtgaaaca accttgtttt ttgagatgga catgatttaa aatttgttaa
    11281 ggcgacaaag atacgctaaa aaaccagcga aacctgcatg atttttgctg taaacggcga
    11341 gccggagtgc tttgccaata cccgataatg attagctttg catcccccgg ccaaaccgga
    11401 ccaaacctaa atttagattc cacatatgaa aagtttcacg ttgaagtcga tgatcctcct
    11461 gctgggatgt atgactttct ttactgccaa cgctcaaaca gcaggcaaag atgaagtatt
    11521 gtttaccgtt gccggtcagc cggttaccaa ggaagaattt ctttacgttt acaagaaaaa
    11581 caaccctgat aagcagaacg actacagcag cgaaagcttg cgtgaatacc tcgatctcta
    11641 tgtaaacttc aaactgaagg tgaccgaggc aagggcagag aaaatcgata caactgagaa
    11701 agttcgcgaa gaactgcaga aatacggcga tcaactcatc aaatcgaact tcgacaaaga
    11761 agtgttggaa ggtgcagcac aacgcatgta tgatcgcatg caaagcgagc gacttgttta
    11821 tcatattatg ataggcatag accctaaagc aaatggcgca gatacagcgg ctgctatcgc
    11881 aaaattaaca gcagctcgtg agcgcctgtt gaaaggtgaa gattttggta aagttgctgc
    11941 cgaagtatca accgataaat caagtcctga taatcccggt tttgttggtt gggtagtgcc
    12001 cggtcagatc aatgacctta catttgaaaa tacggtgttc agcactgctc ccggaaatat
    12061 ttcaaacgtg attaaaagcc gctatggcta tcatattgta aaggtggcta aggaacgttc
    12121 gtctatggga acagttaccg ttgagcatat tttggttcgc gttcctgaca atgcaaaacc
    12181 tgaagacttt gcaaaagcaa aagtgcgcat cgatagcatt gcagctcaaa ttaaagcagg
    12241 taacggaagt tttgaagaaa tggctgcgaa atactccgat gataaaatga ccgctcaaaa
    12301 tggtggtaaa ttggaatctt tcggtaccgg aaaattggca aacgattttc aagaggctgc
    12361 tttcgcattg aaaaatcccg gcgatatttc agcacctgtg aaaacatcaa aaggatggca
    12421 cctgattaaa ctcatagaga gaaaacctat gggcacctat gccgaaatga aagacgaact
    12481 caaaggaaaa attgagcgca gtccggaata caaagaattg cgtgccgatt tcgtaagtaa
    12541 ggcgaaagct aaatacggtt tcactgaaaa tgcggatgct aaaaaagaaa tgtatgcaat
    12601 aatggattct tcatttgtga aaaacagctg gacaatgaac aaagcaggta acatgaataa
    12661 acctatgttc accattggcg acaaatcaat gagtcaggct gattttgcat ctttccttga
    12721 aattaaccag cgttcttctc gcgataaaag cattgaagaa aaattcagta aattatatac
    12781 acaggcagtt gaacaaactg taattgaata tgacctcagc gaacgcaata tcgacttccg
    12841 tcgcttgttg caagaatatc gcgacggact tccgctgttt gctatgctgg aacgcaaaat
    12901 ttggagcaaa ggttctaccg attctgttgg cttgaaaaat tattacgagc aacacaaaac
    12961 agaatatatg tgggaagagc gtatcgatgc aagtatttac acttgtaaag acacgaaaac
    13021 tgcaaaagct gttcgtaaaa tggcttcaaa acatgcttcg actgaatcta ttttagcgaa
    13081 attcaataag gatagccttg aattagtgac tgtaaaaagt ggtttataca tgaccggtca
    13141 ggatagcaat atcgataaga tgaataaaca aaccggtatc ggtgaagata ttctgaatgc
    13201 cgatggtact atcacatttg tgcaaatcaa taagatcgtt cctcctacac caaaaacact
    13261 tgatgaagct cgtggttatg tgatctcagc ttatcaggac caattggaaa aagaatggat
    13321 tgccgaattg cataaaaaat acccggtagc catcaacgaa aaagtattta attcaatggt
    13381 acattgaccc tttgctgatc aggaatgtcg tcatagcatt aatcgtttgc gtttcggttc
    13441 agggttgtcg caaaggcaaa cccgagaaaa atgaggaacc gcttgcgaga gtgtacgaca
    13501 aattccttta tgtgtcagat atagccgggc tcgttagcga taacacctct ccggaagata
    13561 gtgcacggat tgttgatgat tatatcgata actggatacg ccagaatctg atcttgcgcg
    13621 tggcagaaga taatctgcaa accgcaatgg caaaaattaa ccagcaggca gatgaatata
    13681 aagagtcgct ggttctttat gcctatgaac gtcagttcct tgccgaaaat ctggatacgg
    13741 tagttgctga ggattcgctt cgcagctatt acgatctgca taagcaggat ttcaccttac
    13801 agggcgatat ctacagactc gcatacgctg ttgtatccaa agacgataaa actgctgatt
    13861 ccgtgagtta ttggcttaac cgcggattgg aaaagtcacg cctggcttta gaacgctact
    13921 gtgtgaatca tgccgataga tttagtctga ataccgacat ttggctcaac gaaaatgatc
    13981 tttttaccct gcttccatac gatatgtatg ccggtgggcg ttttcgttcc aaggcgccaa
    14041 ttgccttttc agacacggta aatcgttact ttgcaatggt tgccgatttt tatgtggaag
    14101 gagatacggg gccgttcgcg tattttcgca atgatattaa ggatatcatc atcaataaac
    14161 ggaaaaaaga gctgctgaat gatacctatc agcgtatcta tcaggagggt ttaaaacgcg
    14221 ataacgcgga attattaaag aaagaagaat gaaaaagatt gttatagtga ttgccatgtt
    14281 gctgccgata gcggcgtttg ctcagccggt agtagataaa atcgtagcgc aggttggcga
    14341 taaaattgtg ctgatgagcg aagtggaatc gtattactac gatgccctgc aacaaggtga
    14401 agtgccggat gattatcgct gtgccatttt acaagaatta atcactcaaa aattaatgct
    14461 tatccaagcg gaaaaagata gtatcgttgt tactgatgat gaagtaagca gtaatatcga
    14521 taaccgtatt cgctatttta ttcagatgtt cggatctgaa gaaaaaatgg aagaatttta
    14581 tgggaaatcc gttcaggaaa taaaagaaga atttcgagag gatgtaaaaa atatgttgct
    14641 gatcgaccgc atgcgcgaaa gtattgttgg cggtatgtcg gtttcgccta ccgaagtaaa
    14701 agaattttat aattccatac ctaaagattc attgcctttt gtaaatgcac aaatagagta
    14761 ttctcatatc gtaatggtgc cgaaagcaaa tgcagagcaa aaacaaattg cgaaagaaaa
    14821 agctgatggc atccttaagc gtatcaaaaa cggtgaggac ttttgcaaac tggcatcact
    14881 ctattccggt gatggctcaa aagataattg cggtaactta ggctgtgcta ccagagatac
    14941 ttatgtgacc gaattttctg ccgccgcatt ccgcctgcaa ccgggagaaa tttctgacgt
    15001 agtagaaaca caattcggtt accatatcat aaggatggat agtcgacaag gggataaagc
    15061 gtgcttgagt catatactca ttatgcctcc tgttactaac ctcaattttg ttgccgccaa
    15121 caaaaaactt gatagtttgc gcgctcagat catggcaaaa aatataacct tctgcgatgc
    15181 ggctaaaaaa tattccgacg atgagcaaag cagtaaatca tgtggtgttg taataaaccc
    15241 gcaaaccgga tcgggaactt ttgaaatttc ggaattatcg cccgatgatt attattcaat
    15301 agaaaatctc aagccgggtg aggtttcgca agtactacct tatactaccg ccgacggaaa
    15361 aaaagctgtg cgtatcatca tgctcaacga tcagactgaa ccacatcagt taaatctgaa
    15421 agatgattat gccaagctgc agacattcac tttgaatgtt aaacaaaata aagaaatgga
    15481 atcatggatc cgcacaaaag ctgaacgtga gtatattcgc atcgacagca tttacagtgg
    15541 atgttcttca ttgaatgaat tgatgctggc aaacggaaaa taatttcaga acagaggaag
    15601 taatcggtaa catggcaaca ttcaacaacg aagtagaagc aattgacgca ctggcagcag
    15661 actataagaa gctgcaagca gagatcggta aagtaatcat cggtcaggat caggtagtga
    15721 aggatgtact gttgagtatt ttttccgacg gacactcgct attggttggt gttcccggac
    15781 ttgcaaaaac attgttgatt aaaacgattt ccgatgcact gcatttatca ttcaaacgta
    15841 ttcagttcac accggatttg atgccaagtg atatcacagg tgccgaaatt ctcgacgatc
    15901 aacgcaattt taaatttgtt cgcggaccaa tttttgccaa tatcattctt gcggatgaaa
    15961 taaacagaac gccgcctaaa acacaggcag ctttgcttga agccatgcag gaaaagcatg
    16021 taaccattgg cggacacatg catccgctcg acaaaccgtt ttttgtcctt gctacccaaa
    16081 accctatcga acaggagggt acctatccac tgcctgaagc tcaactcgac cgattcatgt
    16141 ttaacatagt gctcgattac cctgcttatg aagcagaact tcaaattgta agagcaacaa
    16201 cttccgataa taaggtaacc ctcaataaag taattggcgg cgaagagatc atggcgtatc
    16261 agcatgttat aaggcgcatt ccggttaccg ataatgtgtt gaactatgcc gtgggattag
    16321 tggcaaaaac acgtcccaat accgaaaaag cggacgcgaa agctaaccaa tacctcagtt
    16381 ggggtgcagg acctcgcgct tcgcagttcc ttgtcctcgg agctaaatgc catgccgcta
    16441 taaatggtaa atatgctccc gatatcgaag atgtgcgtgc cgtagcattg cctgttcttc
    16501 gccatcgtat cgtgcgcaac tataaagccg aagccgacgg tatctctgtt gaagatttga
    16561 ttaaaggaat gatgtaaact tccgtaccgg ctgcatttgg cagggccgat ggcgttttat
    16621 gtacatgttg aggtattcca aagtctgtta ctcacctttt taatgattaa ctttgcacct
    16681 gtatccatta atacttacgt gtgagtgatc tgatcaagca tgaatgtggt atagccctca
    16741 ttcgactgct aaaaccgctg tcctattacc atgagaaata tggcactccc ttgtacggtc
    16801 tgaataaact gtacttactc atggaaaaag agcataaccg cggtcaggat ggcgccggta
    16861 ttgctgcggt gaagatcgat caaaagccgg gatacaaata tttcgaccgc aaacgcagta
    16921 tctcctcatc ggcgatcaaa gacatctttg acgaggtgtt taagaaatcg cacaaaatgg
    16981 agcgcaaaaa tcccgattta taccacaacg ccgaatgggc aaaacaaaat gtccccttcg
    17041 ccggagaatt gctgctcggt cacctgcgat atggcactta tggtcgcaac gatatcgatt
    17101 cctgccatcc cttcgtgaga gaaaataact ggataacaag aactttgctt gttgccggaa
    17161 attttaatat ggtcaacaac gaccaattat ttcagcaact gcttgaactc ggtcagcatc
    17221 caacacaatt tgttgatact gtaaccgtaa tggaaaagat agggcacttt ttagatgatg
    17281 aagtgcagaa attatttgac gagaaaaaag aatatcacga taataagacc ctttcaaaca
    17341 ttattggtaa cgaccttgat attcaacgta tcctggctcg cgcagctaaa gattttgaag
    17401 gaggttatgc tatgaccggt ttgtttggtc atggcgatgc atttgttttg cgagatccga
    17461 atggcatccg tccggtgtac tattatcagg atgatgaagt tgttgtggct gcaagcgaac
    17521 gcccggcaat tatgaccact tttaacgtgc catttgaaag agtgaatgaa ttaaaacccg
    17581 gccacgcgct gatcatcaag aaaaaaggtt cagttaaaga agcgcaatgc cttcaacctc
    17641 gtgaaagaaa aagctgcagt tttgaacgca tttatttctc tcgcggaaac gataaagaaa
    17701 tttataccga acgcaaagag ttgggtcggt tgcttactga taaagtaatg aaatcggtga
    17761 actatgattt cgagcatacc atattttcct tcatccccaa tactgcagaa acggcatttt
    17821 atgggatgat gaagggcttg gagcaatatc tgaataattg gaagattgct gaaatcaagc
    17881 gactgggaag taaaatcacg gaagaggaat tacatcgcat tttaaatatg cgtcctcgtg
    17941 tcgaaaaatt ggcgcataaa gatgtgaaga tgcgcacatt tatcaccaac gattcagatc
    18001 gaagtgattt ggtgagccat gtgtacgata tcacttatgg tcaaattaat gattccagtg
    18061 attctattgt tttaattgac gatagtattg ttcgtggtac tactctcaaa caaagtatta
    18121 tcgccattct cgacagactg aatccgaaaa aaatcatcat cgtttcttcc gctccgcaaa
    18181 tcagatatcc cgattgctac ggtattgaca tgagtaaaat gggcgatttc gtggcattca
    18241 gggcaatgac agatttattg aaggagcaag gaaaagagca gcttattcaa gatgtttatg
    18301 aggaatgtaa gactattcaa gcactgccaa ataatcagac ctataatgca gtgaagaaat
    18361 tatatgctca gtttagtgat gaagaaattt ctgcgcgcat tgctaaaatt gtaaaagctg
    18421 atcatattcg tgctgaagta gaagttgttt atcaatcggt ggaagatctg cataaggcat
    18481 gccctcaaaa cctcggcgac tggtatttca ccggtgatta tcctactccc ggcggcaata
    18541 aagtagccaa caaggcattt atgaactggg tggagaagaa agatgtgcgt gcatattgat
    18601 ttttatcatt cgcaacacaa cttctcaata caggcactaa atttgctatt agcaacatag
    18661 attttctact tatgaatcgc acatttttat tggcatgcct catcatgctc attgctcctt
    18721 tgtcggcaca actctctttc acgggtgccg gggttttgct tcaaaatgaa accgtaaata
    18781 attatcctgt cataaagcag gtattaaaat tctcaccggc atccgatgcc ggattacagg
    18841 ccggagaagt agtgtggtcg gttaatgggc aatctaccga aggcaaaacg attaaagatg
    18901 ttgttgccat gatcaaaggc aataatggtg aatcacgcac tttagtagta ggaagcgaca
    18961 ggcgaactgt agatattacg ataaggctga taaaaggtaa ttgcaccgat ggcgattgta
    19021 acaacggtga agggcgtatt gaagaaccaa atggggatat ttatgtagga aatttcacta
    19081 atggaaaatt cgatgggtat ggcatgtact attattatac cggtgaacat atgtatgctc
    19141 gatatgatgg acatttcgta gcaggaaaac gagaagggga tggggtgctg gataattatg
    19201 atggcggata taaattcacc gggacattta aaggcggaca agcaaacgga aacgctacaa
    19261 tgacatttta taataacaat aatacatata ccggaaattt tattgccgac aagccatcag
    19321 gttcaggaac gctaaccttt gcggatggtt ctaccaaaac attttcacca actacggtag
    19381 atgaaatctt attggaggcc ggtgtgaagc aggaaactca aattgctgat aataccgata
    19441 ataaatccaa taataacaat accgacaata acaacaatgc atcagatagc tatacttccg
    19501 gctacgattt tagtgaaatg cttaataaaa tggcaaatgt tgaatacaat ttgcagtatg
    19561 ccatggagaa atacgcttca ttttatagtg cttataggac aaccttttta gataacaatg
    19621 caactgccgc tgaagaagcg tctgttgatg aactaaaagc ctgcgtcgaa tatgtcgaga
    19681 aagcatttaa tctccttcag gcaatcaata atagttcggc aataggagaa tcagttacag
    19741 agttgcagta tgaggcaatg aaacaatggt caaaagctgt taacgaggca atggaattat
    19801 ttgttgccgg tttggctcag gatgcaaccg tttccacctg gtattggatg aacataagca
    19861 gtgataaaat ggaagacttg cttgatgaag catcggcggc aaggcatgct ttctaatccg
    19921 aaaaagcctt tttcactgat gatggttatt gtgcaatcac aacagtaggt acagtgatat
    19981 tatcaggtag attaataaaa tcttttgcac ctaaggtata cactacaccg ctatcctcca
    20041 gaaagtaaat attataaagg cctgtgaaaa aggatagatt actactatcg taggtttgca
    20101 tccaatatct attaaattcg atagctgcgc cttcaggttc tgaattcaga taagcaaatt
    20161 tttcaaaact attttggtta ccataataat caaaatccga tttttttgaa ccaaacgata
    20221 tcctggattt tggttcggat ttttttattt gttcttctac tatgagtatt tgattgccat
    20281 tgttttctac aacacgaatc catattcctt cagttaatgg caactccgga gccacgttta
    20341 tttcaagtgt attggtgata taagattttc cgttgatgtc aaatttgaaa ggcccaagaa
    20401 tatgtttgcc ggtatcgctg aaaacgaggg tttgggttaa gaatttttct cctccctgga
    20461 aagtactttc aaacgtgata gtgctatcca tagttgaacg catggcgtct agtaccatat
    20521 tcacttgcat cgacaggtca acagattgcc ctacacgtgg tgccggattg ttgagcttta
    20581 gaattaacgt gtcctgactg aacagtatgt tattgacgat caaggaaagc gcaattaatg
    20641 atagcatttt tttcataagg agtattttat agcattgatt tgattacaag aaagtaatgt
    20701 aattaaatta aactctaaaa tcgaatgatt ttaccttgtt caagcatttt tttcgcgttc
    20761 tcccaatttg ggagtgattc agcgtcaatg gttattgttc gtatttcttt agcaattttt
    20821 ttactgttgc gcatagcctc cagatgtgca ccactgcggg caaaggaacg catgtcttct
    20881 tcgtttttcc agagcgacat ggtataatgc attctgccga agccggtttt cttaaattca
    20941 atatgaccac tgcctttcaa ctgtttcata atacgcaagg catacaacga caatggaaaa
    21001 aaagaaaccc atgactttag gcgaagagaa gtgattgttg ctttcatagt ttaataatcc
    21061 gttaattcat gcatctggcg gcaagacaag cagctagcgc taatgctgcg ggcaatgcct
    21121 gggtgatgag aattttttta cttgccgttg ctgcaccata aattccggca caaacaacac
    21181 acgataaaaa gaaaattgca acattactgc tccaaatatt atcactgata atgagcgacc
    21241 atattaatcc tgcagccaga aaaccattat acagtccctg attggcagcc agcgtttttg
    21301 ttggtgtgaa catatgatcg ggtagggcac ctttgaatgt tttcctgccc aaggtttccc
    21361 atgcaaacat ttccatatat agtatataca gatgttctat ggctacgagt atggtaagtg
    21421 tggttgctat tgtttgcata tgcctgagcg cttgatatat tcaaatttcg gaataattat
    21481 ttgctattac aaatgcgatc atgcttaagc acgatgactg atttgcaggc agagcgaagg
    21541 agctattact ttgtaggcag tttatgacta aattgctgtg aataagaact ccggaaacat
    21601 tgatcatttc cgatatcaga atgatttttg ttcatttagt tattgagagc agcatcccat
    21661 ccgccctgct gaagtattgt tgcaaaatca taaagaaaaa cccattaaca atctctgccc
    21721 gcgggtatat ttactgatta gctcttgtaa actatttttc cgtctttctg ccatcggcaa
    21781 catggatggt tacagcaatt cctcctgtta ttgtctttgt ttttgcatgt cgctgaacca
    21841 aatgtgtggt gaatgttgtg ccgggttttg cactttccgg tactttcact ttgaatccaa
    21901 ctacgcgttg cgactgataa ggaatatttg ttgacagacc tgaaactttt tctttgctga
    21961 atggcatttc taaagcattt ccaagttttc tgataagcag atcaggcctt tgatcggtaa
    22021 tcattccaat cgtttgtgtg taatggtcaa catgtccacc gcaatccatc aaagcatgac
    22081 gatcatcaaa tttaaaatga tctttgaggc tgaaatcagg gtgtataatg gtctttagat
    22141 cattgccctc gaaaaacatt ttatccttgt gtaacagact tacttcgtaa ccctccattc
    22201 ttctatcgcg ccacgcttcg ataatatagt cggaaagtaa atcaaagtaa tttgtcaata
    22261 caacaggaat aatgataaat gcattaggaa gtaaatctac cactgttgta tttttttgtg
    22321 ccagattatt atgttcccaa acatgctggt tattaacagg atgatctcct cggcaaagta
    22381 ttgatgcgag tacgcaggca tgtgagccga cagcaggaat attggcgcgg ggccatttcg
    22441 cttttacaat gcgtgtttca cccggaccta atgtaaattc cgctttggcg gcaacagccg
    22501 gaaggaaatc atttgggaat aaaaattgtg tacctgccca tgatttgtga ttaaacgtca
    22561 cgacgaaatg ttgtgcttcg ttcgtagcac ttttgttccg aacgcgtgca taaaaccaat
    22621 tatcctgacc gtattccggc gattggtgtg tagtgccatc atcatcagaa ttgcgaatcc
    22681 ataaatccgg agaatcccag aaagctccat cccattgatc tgaacccgta tgtgatgtgt
    22741 gatctttcat ccataaatca ggagatccga tatcatcaaa cccataaatg aatcggtgaa
    22801 aggcatctac gaggtccttg tttccacttt tgcagataag aaattcttcc atcatttgtg
    22861 tggataccgg acttttacct ttataggtat tatgcatatc gcgcatcaga ttattcgtgg
    22921 tattgacacc aagaatcgct gctattcctt gccagaatct accgccatca tcataagaat
    22981 tacccggtgt tgtccgttgc caggggtttc ttgaacagag tgttattggt gcatcggaat
    23041 agtcgaaggg taatgtatcg ttagcgccat catcgtgaaa agttgtaaag gcttcatccc
    23101 accatccatc acattgtgct gcgggtttca tacctcgggc aaaccagctg tgaaaagttt
    23161 catgattcaa tgcccagctt gcagttgttg ttccaccttc atattccata cctccattga
    23221 tggtagtaaa taaggcagta aatttattgt cgtgcaaata tgcaccatat gcttcttcgt
    23281 tctcagccag aaatgtggca atattgttga tctcagtatt taagtttgct gcattaccca
    23341 ctaatttaaa tgcttctatc gtaatatttt ttcctgatac aggaagaaca gtattacctg
    23401 tttgagattc gacggtcgat gtggcgcgaa tttctaataa gggagataca caactgaatc
    23461 ttgaaggaaa tgataattgc cagtgatttg ttcctactgt agttactgtt gcatttgtaa
    23521 ttaccgtatg tggaaccaaa gtattcgtaa tgatgatatc taaatcaatg ctgtattgat
    23581 caaatggaag attggcaggt atccatgctt ccagatatct tgctgcgttt aaatctgaca
    23641 atccgaaaac aaatttaagc gaggggccgg caagccagtc aagaacggga atgtaactgc
    23701 cgccaagttg cgattgcgga gtgcctaaag tgtattgcaa ttgtagtgtg tgataagaaa
    23761 gtgccgcctg aacatcatca ataacacgta atgtagtata ggtgccggta tcgaaattat
    23821 gcgaggctaa ttgtgcaaca ggaaatgctg cgccatcaag aatggcagaa gaaatgtttt
    23881 gcctgagatc aaaaaatggt ctgcctgccg ttggtcctac gatataatcg atagttatgt
    23941 ctgctaatgc aacagaagtt gcggcattaa atgttatgct tccgttgatg gattgaatat
    24001 ccatcggaac ggcaaacaag ccatcaacag ttttaggtgg cggggcgatg tgaaaatttg
    24061 ttggcatgag catatgtttt taaaataaaa cggcagcact caaaattgaa tgctgccatt
    24121 ttcagatgaa tttaatcgca ttgacagttg tcgctattca attcgatgtc gagcgaaact
    24181 tttttaatgc agactttttt cacttttcca ctatttaaat ggaggcaatc aagcagatcc
    24241 tgtgctttat cagtgcagcg atcttttttt ccgggaatgt atccgggctc ttttttgtcg
    24301 ggatcgatgc gatatgtcat tccaccaatc aacatgtctt cagcatataa tccgattaca
    24361 atatcgcgct gataggcggc atgaacttgc tctgtggaga agtcgcgacc tttaattaca
    24421 tcaatcacca ttttgcgttt ctcgcccggc tttaatgtaa attgatcctt cgagtctttg
    24481 aattgcactt tccatcctgc ttcggcaagg aattttggaa gctcaacctt caattgcatt
    24541 tttgtttgaa ccctgaaagg atttcccgcc cagaagaaat gatcatgcaa gcttgcgatg
    24601 agcgcatttt ctccaccacc tccgggaagt atggttacgt tgcgttggcc gatattatta
    24661 tcgttaggaa ctaaacgcca ttcagcaaca gtatccccta acgtgaaatt atctacgttg
    24721 cttggatcac catcggtaga tacgatcatc aaggtgcaat cgtgaccata tatattttca
    24781 ttaggcaccc attcaaatgg tccgacaatt acttcttctg cattatttgc agcaatggag
    24841 gctacgttta aacctcctcc gggtcccatt tcagtgaaat ctgttggcca ggttaaaccc
    24901 gcacccggta agcaatggta tccgcgaaca tttacattgc cggaattcga ggtgccgcga
    24961 tttttaactt tcacatacat gtaatttgtt gcatcgggaa tggcatcctc atgtgtcagg
    25021 ccgccatcat catgttggcg attccacatt gagatattat gccaatgcac agcctggtat
    25081 tcatattcac catttcttcc gtcattgata tatacatcaa cttccggcgg ggcacctgca
    25141 gtagttacag gcattggagc tccggccggt tggaataatc cttgtttttc aaaagcccag
    25201 cgaattactt tgttataagc accgcctgat atcccttctg atgtccagtc aagtaaatca
    25261 accgccatca acgcatcaca aaatccttga gcatgtgctg gatttgtttc aggtgtcaat
    25321 atacttacgg cacgcaatat taaataggtt gccatgcgag aagcaaactg tcttcggctt
    25381 aaacttgaag agtcgccacc gatagaacga taccagcgga aatttgatgt tgcaagaatc
    25441 tgttcacttt cgtaaccacc aatatcattg ctgccatccc atccccagcc ggcagccaca
    25501 tcgcggttaa accaacggtc aagagcagga tacctgaatg gtgcgtaacg aaatctttca
    25561 atattgccta gttctcgtaa ttgagatgta gggtcatttt gaattgccgc aaggccatca
    25621 ccggcagaat gcgcaaaatt aaaataccag gtcccaacat gatcatataa tattccatga
    25681 ccacctaatt catgccaatg cacccaattg tcaggagcac gacctattgt tggatcaacc
    25741 atattggttt catctccata tccatatccg gaatgatcaa taccatctac accatttccc
    25801 atgcaatgag cattcacatc aatttgatca gcatgatcaa tatctaccgg gaaagatgta
    25861 tttgtgaaat aggtagacaa atcaaaacct aaactctcaa tggtttcgaa aaaattatca
    25921 gtatgataat atgcgcttac tgcagcaaaa tcatttgtgc gaacattgta atcaaaatca
    25981 tcacctactg cttttgttgg tggtggttca gttctgccgt ctacatcaac tagttgcaca
    26041 tttgatcccg ttaagctctg cacaccacca acaggtgcat ccagattatt taaagtaacg
    26101 gaagttcggt atggatttaa ggttgcatta ttgagatttg cagtttgtgt tgtatcccct
    26161 gaagaggtaa tggggtcttg cacaaatact ttgccgctaa ctcccgcaaa caatggttcg
    26221 atgtatagga tgcttccggt ttccatttca accaatgcaa gccatgtttg catttcgttt
    26281 gcaattttcc atgcgaaagt gatttcagca accagataat actgtccgtt tttaatgctt
    26341 ttatctatcg gaggcaaagg aaatgatgga tgttcatgtt cttcaccgac acgcaccttg
    26401 tctttggtta tattatggct atgcactgct tgacgtttat tttcatcata agcgtaataa
    26461 tacatgcgac cacgtataag acgagccgga tttctttttc ctgcctgcct gtctgttttg
    26521 gcattggctt tgatattcat tgcaaagcca cgcacgaaat ctcctgtttg cgggtcgtcc
    26581 ttttgccctt cttcagataa caatggaaca atgggcttgt gtgcaatgga cgcaaacatt
    26641 gattgatatc gagaaatcgc tttttcagag gctaatttta tgcggaacga ggcaacggta
    26701 ttattcgctg catacacaat gcgattggga ttctgtttga ctgtaacggt caatcccttt
    26761 ctccagattg gaatgttgtt gatcgtttgc gccatgcgaa tggtgtttga atcaaaatag
    26821 cgcttctcgt caacgattcg caattccatg tcctgttcta ttggagtatt atatgcaagc
    26881 ggcttttgca tattttgtaa atgactttcg ctgatcttga aatcatcggc catgtttcga
    26941 atgtagtcaa atgcagctga tctcggaatt ttctgattgc ttaatgccgg ttcctgaatg
    27001 tgcaaaacct gtcgggcaat gcgttttttg tctctcgtta caaatgcttt taacggcgac
    27061 tcgtacttgg aattcataaa ttggttttta tggtgaaaaa atttatagcc gattaaaatt
    27121 cttaatcgac ttttcaaatg tattcaggct tttaccgtaa aaacaataga gatagttcaa
    27181 attcacggtt taccgttaat tcgaccaagt cacagatgct gtaagtttaa atgcttgttt
    27241 ggcaagcctt tcaaaagaat gaacatcgaa ttaaatgtcg tatttaattc aaaaaatgtc
    27301 acttttttga aaaatgtgta attttttcat taacggtttc cggttaataa aaaattgaca
    27361 attttatgtg tgaattattg tattcactgt tttccgtgaa caccattctt atcaaaatga
    27421 agaaacttat acgaatccgg ggactcagct atttgcttta tagtattatt gcgctcaacg
    27481 agaaatttcg tcatgtatcg tgttaaaaat atccggtcaa ataattttcc caccacacca
    27541 aaaggcgagg taaattcaaa cacatcagtc ataattgtag tatgcgactg tgcttcgaag
    27601 aaatgatcgt gtttgaaata gtcgaaggcg cctttcacca tttcatctct gaaatgaaac
    27661 ggtctctcaa aaatggtgat ctttgctgtt aattgttgcc tgattccaag gtgtgttgct
    27721 tcccatgtga cactttcgtt caaagaaatt aatcctgagg tgacacctgc aactgctacc
    27781 tcatttgtcg acttggtaga catcatatgc agatcaattg agcgcgaaag atcaaacgct
    27841 acttcgatat cgcaatgtat agttgttgtc aatcgaatga tgggcatgaa atcatttatg
    27901 cagtcggctt taaagataga cttctaaaaa ttgaattgga aataattgca cgctcactgc
    27961 ataatcttca aatactcctt ccagggtcct acatcttcgc gatattgttt tgccattacc
    28021 cttgaaattt gcataatgtg atccaggtca tgcgctaccc aggttgacag aagttgctgt
    28081 aatgttacct tcccgaattt aggatgtatg cctgtttttt ggagatctgt tttgctgata
    28141 tttttcgaca ataaataggt aatatttgat tttctttcat catggaaagt attcaaaagc
    28201 tgttctaagg attttccttt actctcctga aattgtgcaa atctgtcgaa ggttgcaaac
    28261 tctttattac caccgtctga aagtataatg tccatccgtt ttgtccaatc agtagtctct
    28321 ccatgaatca gatggcctat aacatcatat ggactccatg tttcaggacc ttcgttgacg
    28381 ccggtccaat gctcaggtaa tcctgacaac aattgtttta aaactagcgg cgttctctca
    28441 agtatttcta tggagcgctc tatagaaaat gacataaaat gatgttttga ttattttaat
    28501 cgctccaatt taccttttcg tttcacaatg tttttatagt cccactgaat ctcggctgct
    28561 ttgttaagcc aacgttttaa atcgcttttc tgaatttgat ttacattggt aaaacgtgct
    28621 tcagctgcca taaactttcc ttcaggtagt aaatcgggtt cttcaaatga ttgtccgctc
    28681 caaaataata acctaacaga atctttaagt ttgctgtagc caactatggg gtttccatca
    28741 atgaaccata ccggatgcgc atgccaaatt ttgctttctg catttggaag atgcttgtcg
    28801 attaatgaag ccagtgtatc agcaattgcc ttgtcgcttt ctgttaaggc ttcgtgatag
    28861 atatttatgt caggatgcat ttgttaatta tcttgattta atcgactaaa attaatctat
    28921 attcttaagc cggcaagaag tttaactttt gacgaatagc tttaatatgc ataatttgcg
    28981 attttcaaaa tgcatttcaa gcagaataac ctaggaaccg gagtttagca gataattgaa
    29041 atgcgaatca atcctttaat ccatctgtca gggatttcga atcgatatat acatctttca
    29101 aaacggtttt cccattttca gccattacca cagcataggt attgtgacct tcagcaattg
    29161 catttctgta tgcagtttct gctgcctttg ctatggattc ttcaacataa aattttgaga
    29221 atgggtattc aatctcaaca taaggttcgg gagagcgact aatgtgttta acagtagcag
    29281 ttacaaagtt gatatccgga cggggtttgt ccttttcata gtgactatag gtggcaaact
    29341 gaccttcatc aacattcaat acaatatata tattatcacc ttccttccaa tcatctgcat
    29401 ctgaaactgt aatgcgggta ttttcgaaat tgagtgcgat ataatttcct ctgaacaggt
    29461 ctttagggtc aacgggaata gttctgaatt tcagcacttt accgtatgat aatgtttcag
    29521 cactctcaat ggcccagctt gcgggtagat atatctgtag cgctgctaat aaaacgaaag
    29581 ctacaatggc aattttattg gttttcataa atcctccttc ttttaagcat gatgacatta
    29641 aaagttacaa atgcagcgcc taccagcata aatagtacac cctttaattg ataactaagg
    29701 tcggattcga aaaatttgta aatagccagc acggcaataa tgctcagtcc aagattcatc
    29761 aagcctaaat gatttctttt tgagcctaaa aaaatgaacg ttattcccgc tgcaaatatg
    29821 tagatattat acaatatcac agccacaacg gtgaattcat ttaatacata tgtagccagg
    29881 aaaatgaagc cggtatattc ccatagggct agtttgcttt ttcctgccca tcggtgatta
    29941 aggaatgtaa caatgcctga tgtgataatt aaagcaagtg caaaaataaa cccatcatcc
    30001 ttaacgaagt taaatcgatg aattgacttg taaaacccac ggtcgaatgc tatgaaattg
    30061 cttgaaataa ataatattac cagcatacca agaaagccga taattttgaa accatttttg
    30121 ccggtattaa ctgctgaaaa aaggcttgag ttccctattg caaatattag actgaaataa
    30181 ccgaaataca taagaataag tgcttcatcg taatcttgcg ttaatagtgt cagacatagt
    30241 gacatggaaa tgggaataaa ccaattgtgt aatgacgtga agttgttgat agatttgcgt
    30301 tgcagtaaat ttatataata cggcagggcc attgcaatca acagccaata cagccgtgag
    30361 aaaccttcat gataatcaaa tgcgtattta cctccatacc atgtaatacc tgcgatatac
    30421 agaagtgacg acatgggaga acgcataata tacacgattg gaattgatag caacatccag
    30481 attaaaagga ttttttcctc agaatatttt aaatgataga tttgagaaat cagcgcgatg
    30541 caagctccta ctgaaaaaaa caggaaaaca gaagccgctt ccttccaaag tgattcgagt
    30601 ctatttctga atagtagtgt tccgcataat atttgaccaa gcaccaaagg tataaacgca
    30661 aatattgtct tttgcaaatg tgagaataag tcccaattgt gttcaataaa taaaatgagt
    30721 ccactgccta ccaaaactgc tcctaatatc ccaaaggcta cgaatagacg ccgattgcct
    30781 tttgtggatg cttttttatc atagtattcg gatacctgtt gggctgtttc ttcggtaagg
    30841 ataccggctt caaccaattc atgaagatct tttaacatgg ctgtgtgttt tgatgcaaag
    30901 taatccatgg attataattg ccggctatga aggaacgctg ttgtttgata tttatgttac
    30961 ggaaaatcgc aaattttccc gactttaccc aatcttgttg caaaaaatgc aaatttaagg
    31021 cttgagccct tctgagtcac ccggtaaagc tgaattttcc tcttttcgca caaatctgtc
    31081 ggcgacttct aaaatcgcaa tattggaaga taggaaagag gagtgaggag aaaggagaaa
    31141 ggagaaagga gaaaggggtg ggggtagagg agaaatgcaa aatccatgtt atatccgaag
    31201 atatttcatc ccatcaattt agattttgtt ttcacttagc cgcattgaat aactaatacg
    31261 gtacctgaat tattataaca tcacattttc ctacaaacgc acaaacgaca ggctctgaag
    31321 aatatctcct ccgcgattaa tgcggacata ataaatggcg ggagctaaat gcgtgacgtt
    31381 aagtgtggta gttaaagccg attctgacaa attgccgttc atcacaaatt gacctgtttc
    31441 gttatatatc gaataggtgg catctttcgc tagcgtggca ttgttgtgtg ctatagtaat
    31501 tgatccgttg cttggtgacg gatatacatt gaattgtatg gtagtgccat cgggttcttc
    31561 cggctgctta aatactgcag taaatgttac ttcggcatca ctgatgtttg atgtaaagtg
    31621 tgcattcagg gtatcgatta taaaggcact tggctcccaa tatgaaaata tatatccgga
    31681 atcagcttgg gcagtaagtg ttattggcac accatcaaag tatattcctg tccacggata
    31741 gactcccggt tctatggtgt tcagatggat agtgcctgcc agttcaggat aaacatcaag
    31801 atgcacggta acttcctttg taaggtcgaa ttcacttaac aggtcgcccc ttactttgtc
    31861 acttctgcat tcgagttgct gacggaatgt ttcatgcctg gcggtgaaat cggccatttg
    31921 accttctaca ttgagtgggt ctccccaacg cgcatattca ttcggcattt caggtgccca
    31981 tttgtcataa attgcctgtt ctcctgcaag cagtgtatca attaaataag cagtattcat
    32041 taaatcggca aagcgattga taaattgatt gagatatgtt tcattttgaa tgctttgtaa
    32101 ccagatattg atatatggat tatcggttcc ttggctaagc atgtatgcaa tgtgattata
    32161 tgaacagttg gtccaactgt ttggcattag tgaaagttca agatcaatga gtgcgaaacg
    32221 ccaacgccaa tttgttttat cggaacgata tattttaatg ttgttgtaag gccagtcggt
    32281 atttcccatc catgattcgc cgataatata atccgtataa tgttcaagat cgaaatagat
    32341 atcggcttga tcgatataat cagtatccat aggatttaat gcgttgaagg cattgtaatc
    32401 ggcatagaaa ttgtcgacat cgccttctaa tgcacgtagc aataaattat agaaatagct
    32461 cagtgaaaga atttcagtcg aatcttcatt tatgtcctcg cgctcatcga aatattcggt
    32521 gttgaatttt tcccgcaatt catatagtcc gaaataggaa ccgttgatat acactgaaac
    32581 aggctccatc gcagaataat aattattggt gccgttgctc attaagctca cctggcaagc
    32641 atctttatag ggtaatgtca gccattggtt actgccattg cgtaaataca catcactgta
    32701 atcattgcga aatggtatag cgggaattaa ttgctcatgg atggttttct caccaagtgc
    32761 tgcatgatcg aaacttaagc gaaaagagtg ttgaggattt gctctgctac cgccggcacc
    32821 tccatccatg cgcatagctg tgcgggtttc aaataaaatt gggtgaccaa tttcctttga
    32881 aaggtatgcg gcatgtgcgg cttttatcca gtctgagttg taattgtcga atataccggt
    32941 tgcgccatat agattaatat tatcagtagt aaccagcaat ataggtgtgc tgtgatttac
    33001 gccgaataaa tatgtggcta tggcatcgta actaggtaaa taagttgcgt ctgtttccgg
    33061 aaataccctg gcccttacaa caatattttc attgatcgga attggtgtgc cggtgtaaat
    33121 ggttgatgta aatgttggtt cacttccatc caatgtgtaa acaacccttg ttgccactgc
    33181 acctgtattc ggattagtga tgatcaaatt aaaggcatca tctacaatgc tcgtcggaac
    33241 tgacaacacc ggagatataa gcgtgtctgt aaaaacattt gcggcattat ttgtcatgcc
    33301 gggtgatggt gtcatccaaa caatatcttc ggttccatcc ggacttcgac caaccgaaat
    33361 atcagcaata ggtgaattta ccatcagtga acttaccaag cttcctgttg catcattaag
    33421 ataaatattt tcgccatccg catcaatctt gaaattagtg tgatcagcat agccgttgac
    33481 aggaatcata ctttctgaaa gtgcatcttc tgaggtgctg ttgatggcaa tatctatata
    33541 cgtatatgtg attacgtttg ttgatgccac ttgaaatgcg agtgaagaaa ttggacctgc
    33601 gcttaatcct gcggctatca gttcttccgc acgcactaat atctgatgac gcgcactcca
    33661 gtaccaattt ccataaggag caggataatc aacatttgaa tttagtatgg agccgaaatc
    33721 aattgtgatg ccgttaattt gcagatttgg tctttgaaaa taaagtgttc caagtacatt
    33781 gctgcacgat gcatcactgc catcaacaaa tgttgcagat gttgaagcat atggtgtggc
    33841 cgtttgttga aataccgaat tttcagtgta gccggtatta ttatatgagc aaatattaaa
    33901 aatgagatta ctaacaccat cccaatcata cgctgttgta aaataatgtg tattccagcc
    33961 gacttcaggt ataaaatcag tttcatcaat tgctaatgta aatggtgttg tatgatgacg
    34021 atctttctca ctgcaataga ttgttgtaaa tgcatgtggt tcaagggtaa aatcggtcaa
    34081 tgaccatagt gttggttcgg aaactttatc acttaaaaag tatccactca ggtcggcagg
    34141 aacatctgag ttattataca gctctatcca gtcggagccc tcaccatctt catctatacc
    34201 cgtcaggtag cttttgttcg agccttcgtt gatgatgatt tgtgaaaata aatagcctct
    34261 agatacagca cctaaaagga gtaataacaa taggaatcgc gacagcattg aggagtaggg
    34321 ttaaactttt tcgggtttgg gtttggatat attcaaagtt aaggataatc caaatatgtc
    34381 ctgataaacc gattgtaaac tgattatttt gacctattta tgaaatatgc tgctcggtat
    34441 gttgcatgaa acgacatgtt gtagaagatt tgattcaaca aaagcattcg aggatgcatt
    34501 taacgcatat ttgttgacaa tcaacaaagt gatttaccct gatgcgttct cataaaatac
    34561 ttacaagcta atgtttagtg tcagtgaaat tgatggtgta tagatattgt ggatagtagt
    34621 aagactaggc tctgcaacat tttcaatggc agggtagtcg cttgatatag gtatgtatgt
    34681 tcgggttgat tgaaactctt caatagcaat taaaatggat gtttcagtgg tcaggctgat
    34741 tcttggcgtg caatataacc gaagtcccag cagaggcgct aagccatatt ttaccagtga
    34801 agattcagtt ccgttggcat atccggcatt gtagtaattt agatcaaagt cgatacttct
    34861 aacgttgaaa attccgtcga gaccataata aatttgccag cgtttatcga gcatcttaaa
    34921 ccattcatat cccacactta attgcagaaa agtttcacta ttttgataat gattggaatc
    34981 ttcgtcgtaa tatgatttag gattttctaa ggtgaatgac acattaccgg cgatacggag
    35041 agagcctttg tcgaagagcc tcctataaat catcaaatat tcaggtgcat cgggccaggt
    35101 catttcatta aaattcaaat aggatctgat tccatacgcc acatccagac ctatttgatt
    35161 atgttgtaaa ctgtctggtc tcgattgaca aaatgcgaaa ccggagtatg agataaatag
    35221 acttatgatg attgtttttt tcattgcttc gtgtttttgg ggattaaaga tcaattgtaa
    35281 gcaccaatgt atatggaact tcatagcccg tgtacaatga tttttgggtt ggatcggttt
    35341 catccggcag ttccgggtag tcagaagatg ttggtgtata tgttcgatga acatcaactg
    35401 tattgaaatt tattttcaaa ttcgtctcag tggtaaggct aatgcgtggt gtaaggtcga
    35461 accgcacgcc aataaatggt ccaatagaat gcctcagatc ttttgttttg gttgaaatgg
    35521 catagccatc atcgaaattg tcggcttcat aattttcaaa ttgcatgaag gtgcctatat
    35581 cggctccata atatacccac catttttttg atataggtgt atcccattcc cagcctgcag
    35641 aaaaagcaac atcataatcg atattggtat acaacgtaga gtcatagtcg taacgactga
    35701 caatttcttc actgttataa ttggcgccaa atgctacgcg aatattgcct ggtgccagga
    35761 ttcttcgata gtatagcata tagcttgggt attggtaata atcgaatcca tcaaaattaa
    35821 taaactgtga aacaaaatag gaggcattga tgccgagttg gtttttatgc aacatggggt
    35881 catcggtaaa cgcattttga ctaagcgcga atctgctaat cattaataac aggaaacctg
    35941 aaagtgttga tttcatggcg taggctttta gtggaaacta agttatggct tatccttgaa
    36001 cattcaaagc gaagaatgtc acttaatttt catattaaag gtaatgaaaa cgtaaccttt
    36061 ttgcttgcag gtgtattatt tcacatcatt acaccttgag acttattaaa tttgagtgta
    36121 aagggttaac tttaataaat gccaattcat tataaatatc taataggatc tattgctgcc
    36181 tgtatttgtt acgtgcactg ctatactcaa aactacgtgc ctcttgttgt agaaggtaat
    36241 acatggtttg aagagtatga atatatgtcc gcttatggtg acctgacttc ttttttgatt
    36301 gatcaggata ccattattga cgatatgcaa tattggcgtt tgaggaagtg ggtgcaacat
    36361 tcgagtgccg gctggatggg tcaagtagaa attaacccgg tttatgaaga cacaattcca
    36421 atttacttcc ttagggagga cactgttgaa agaaaggttt atttgagaga gcaatggccc
    36481 ctcattacaa atgaagtgtt gatgtataat tttaatctgg aggtagggga tactttagat
    36541 ttcggcgaca cttccgaggt gaaaatcgtt acaagcattg atagtgtcct tatgaataca
    36601 aagtggcaca aacgaatgca tattactccg gaatacgctc ccgtagagaa ttatgttgat
    36661 ttaattgaag gaattggatc taccttcggt ctaaatcagg gagttgaatt tcctttcgaa
    36721 gaaagtatgc ggttgttatg tttttctaat gatgattcat tgtatataaa tttttatgta
    36781 gatgaaattc ctataagtgt ggattctgct gagatttgtg attttctgcc ttccaatgtt
    36841 tcaatagatg ggctgcaaca gcagctgaat tttgatgtat atcctaatcc ggttggacaa
    36901 attatgcata ttgatttgca tgcctcattg gtaggagtaa acgacatcct gttatttaat
    36961 agcattggtg aattatccaa acatttattg catattgatt ctgcatctct tgagataaat
    37021 gtatcagatc tgttacccgg aatgtatgtt ttggtagtaa ggggcattaa tgcggcggct
    37081 attagtacta ctgttattat tcaataatgc gccttgtgtg ttgttaaccg gaaattgatg
    37141 actatgtttg actgaaatac caatcagtct ttctcccatc ttcgtgtaac agaacctgca
    37201 tgcttttcga ttgtccaatt gttttgttcc gctttttcga gaagtggttt tctcagcttc
    37261 tttgggtaga atattttgcg atatgttttc tggtgatcat actcagttaa tattccaagt
    37321 tgccctaaca catcgtaagc agcggcgcca
//
LOCUS       DAOQOU010000009        36293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_471_length_36293_cov_5.654699, whole genome shotgun sequence.
ACCESSION   DAOQOU010000009 DAOQOU010000000
VERSION     DAOQOU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 36293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36293
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_471_length_36293_cov_5.654699"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLM90_01505"
     CDS             <1..950
                     /locus_tag="PLM90_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ88070.1"
                     /translation="ALSLAVISLLLGACKSKLEKKDQSLDEMQEDAGKQENYVPVLES
                     ADATPVYKKIGDSVEMHLNIYYPKKHDKQAATPVIIYFFGGAFIHGSPGQYEDHCKYF
                     ASRGIVAITADYRVISRNKGNAINCVVDAKSAVRYVREHAKELNIDPNKVIVSGGSAG
                     AFLALTCNMNDPKWQDSTDNAQVSCAPNAMILLNPVVNSMEHKFRIQKFKDDAKQPDS
                     ESHAAEINPLTSIRAGMPPTIIFHGTKDKISGYQFVKQFADDYEKAGNRCELVTYEGQ
                     KHGFTAIKFEDGKYYRDALKKSDEFLISLGYLSGKPTVE"
     gene            1063..3519
                     /locus_tag="PLM90_01510"
     CDS             1063..3519
                     /locus_tag="PLM90_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HMZ88071.1"
                     /translation="MRIFVSLFISGLLVTSVNAQTIIKREHSAVPINENIHIDGVLNE
                     ASWNQAPIADNFTQFQFKYNMASAYKTEVRVLYDNTAIYIGATMYDPAPDSIQVGMTQ
                     RDEFATADFFGIILDPYLDGNNALEFFVSAAGVQGDAKAYAGAGNFGEDFNWDAVWAS
                     DVKISEIGWVAEMRIPYSAIRFPNVDVQTWGVNYIREVKRNRDKSSWNNFDPQIDGFV
                     KQCGLLKGIEHIEPPIRLSISPYFSSYYDLYSDKINDISSTAWSFNGGADLKYGLNDA
                     FTLDMTLIPDFGQVQSDNQVLNLTPFETYYVEQRQFFTEGTELFNKGDLFYTRRIGGT
                     PYGYYDVYYGLDTTESVIDNPIQAHLLNAAKVSGRTSKGLGIGVFNAVEGETYATIEN
                     SEGEKRTELTGPRANYNVLVFDQQLKNNSYVSLINTNTLRDGNYQDANVTGMEFLVNN
                     KSNIWGVEGGAAYSSIFDADAEPTTGIHHRIRGGKTGGKRRFILGYDYIGSTYNINDL
                     GYLPINNQLNFSFNTSYNSYEPLWHLNQIHMVFETNYLRLVDPEAFQNFSIYGETYGS
                     FRNYMYAGVFFYTEPVTTYDYFEPRVPGRFYTYPVNYNFGGWLSTDYGKRWAIDINSN
                     YRVFDDEGRYRWNVSMSPRWRATDRILIILDAGRYYWPGDVGFVNLMGDSIIMGRRKN
                     VTMEGVLSVNYAPLVSMNFNMRLRHYWSFAEYSDFYNLGENGDLLATTYNSFLPDGTS
                     SDDVNFNAFNVDLVYTWYFAPGSELNVVWKNAIYQFGTLLPENYVDDLNQVFDSPTNN
                     NFSIKVLYYLDYLYLQKRKA"
     gene            3534..4946
                     /locus_tag="PLM90_01515"
     CDS             3534..4946
                     /locus_tag="PLM90_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88072.1"
                     /translation="MLRHLLSLAVCLLGFGIINAQTPAIDVHFGDIHSINRGSSSVLD
                     DAGGVWFAGTISNGAEGSDDIAIYRIKQSGEILWWHAYGDARNEYVNTIAAGGDGILY
                     ACGDVRDPETEDLNAFIMALDTAGNVIWNQEYGEAATNEEFYQLAIAPDATILLTGFH
                     SALTGTGNDVLLARYAPDGALLEDTVFGTDRNEVGMSIVALPGGNIFVSGDRQTIDGY
                     YNPIFIKLSPDFNVINDIDLTSPHNSGCKSMHLNFQGDLYISGETASFATPEFDVLFI
                     VVDTLGNLVTQTGIPSAGPDAGYDIVQLPDSTYAVTGFAYNPAKGENDMLLMHIDSSG
                     NEIDRLFFGETGADMGYDIKVDAIGNCRLSGFHTNADTTFFSLVIAGFPAQTETENIA
                     SPERFLSINPNPITESICVQTELEVDRVIVYDNLGRRVGDLPKTSGQCFEWNTLLPKG
                     IYYIQMYTSVGNFTKKIIAG"
     gene            complement(4985..5491)
                     /locus_tag="PLM90_01520"
     CDS             complement(4985..5491)
                     /locus_tag="PLM90_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ88073.1"
                     /translation="MKQIKWMLVVAVMAYSSIASAQQVKIAYINSQELLSSMPEAKAA
                     NDKLVAYAREFDSLYQDYVQEYQMLVNDIQGNPNLSEVAREAKIQDAVMLEDRITSYE
                     KETQQKLDAKKQELFEPIIKNTTEIIKTVAKENGYTHVIDNSLSVVIMAPEGDDILPL
                     VKKKMNIQ"
     gene            complement(5558..6514)
                     /locus_tag="PLM90_01525"
     CDS             complement(5558..6514)
                     /locus_tag="PLM90_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88074.1"
                     /translation="MRNIISGICTILICFNSISFAQSTDIYLFIDSNTIIGKISDNKL
                     FTGPSEVAYTLSGNTVYVGDSTSKEQILFLVNAKDMLHRKSGVILQADGSTVQYIAMQ
                     NSFYLGDHPIDKDNEELLHFEVINDSLTEVYSGISDLLIGVIEGKLTSQSQVTAVAHQ
                     YIRHFSLDDSVRARMAQFSVDIVTDGKSFIHPKLDMGPYYEWVWDGQTLKPLWGYRPE
                     DEWVFDGKYLKPAWNADPQTEWVWDGKVLKPFWESGVDKQWLWDDAMLKPYWDSNPDL
                     QWTFDEGVWRPMWRFNDAEEWVVEGYVPVPVAALVVLGMADR"
     gene            6517..7629
                     /gene="mtnA"
                     /locus_tag="PLM90_01530"
     CDS             6517..7629
                     /gene="mtnA"
                     /locus_tag="PLM90_01530"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HMZ88075.1"
                     /translation="MLIKGTQFRTIWLHPDDENCVQLIDQRWLPHEVRIADIRTVDEM
                     CTAIADMWVRGAPLIGAAAAWGMYLAACEVPASCARDFFFEEAYGKLLKTRPTAVNLK
                     WALDEMLTAITGNDSIIESKWYARNRAKWIADDDVKVNTAIGEYGLSLLSAIAEKKSG
                     EPVQILTHCNAGWLATVDRGTITAPIYLAAEKNIPIHVYVSETRPRNQGAQLTAWELG
                     ENNIPHTLIADNAAGYLMQQGKIDIVLVGTDRTSARGDVINKIGTYLKALAAHEHQVP
                     FFVAAPSSSIDFTMKDAFTEAKIEERNADEVTYVQGRADGKIISVRISPESTTAINPA
                     FDITPAKYVTGLITEKGICSASFEGLKKLFPEKFSF"
     gene            complement(7630..7932)
                     /locus_tag="PLM90_01535"
     CDS             complement(7630..7932)
                     /locus_tag="PLM90_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88076.1"
                     /translation="MTRYLIGLIIATALFSSCETDPPANALQLDTGYLRNYTGLDGCG
                     WVIINADNQAMEPINLGDFGLELVDYAPISFTYTEVNSVSICMVGPIIELVTVKME"
     gene            8029..8640
                     /locus_tag="PLM90_01540"
     CDS             8029..8640
                     /locus_tag="PLM90_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HMZ88077.1"
                     /translation="MTHDGYIKYEQHWEIAKPLTDIEPLVLMRNQLAALQWIGVYPDG
                     VGYGNISLRLEGADQFLISGTQTGNMLIADASHFCMVEKADINKNILWCKGPIKASSE
                     ALTHAAFYQSDRNICAVIHIHDAAAWKRLLFVNPTVSKEIEYGTPEMAFAMQALIHSQ
                     QNKLCGVIVTAGHADGIFAYGDSLNSAFSVLESAMHHSPTNNG"
     gene            complement(8616..9449)
                     /locus_tag="PLM90_01545"
     CDS             complement(8616..9449)
                     /locus_tag="PLM90_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HMZ88078.1"
                     /translation="MKLSLAYSPCPNDTFMFDAIAHGRIDTEGLTFDIHLADVESLNQ
                     QALEEKHHVSKLSFHAFAYVSHQYKLLTSGAALGRGCGPLLISKDEIPKSKIEFCLIG
                     IPGRLTTANFLCTLAFPEAATKKEMVFSDIEQSLLMGGIDIGLIIHENRFTYQQKGLR
                     KIVDLGEWWESKYHMPIPLGGIAVSRELPIEIQEKVNRVIQRSVAYALAHPEMSKDYV
                     CAHAQEMSPEVMQQHIALYVNDFSVDLGVEGKSAVEQLYHVALEKKIIGSLRYPLFVG
                     E"
     gene            complement(9446..10129)
                     /gene="mqnB"
                     /locus_tag="PLM90_01550"
     CDS             complement(9446..10129)
                     /gene="mqnB"
                     /locus_tag="PLM90_01550"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HMZ88079.1"
                     /translation="MSILLTAATQFELKPLIDKGSTTSTGSNSFKLVVPGFPDIEILI
                     AGVGTAATVFHLTEMLMKKKFDLAIQVGICGAFDPSLSKGEVVLVQSDCFAQLGAEDG
                     DDFIDVFDLGLTAHNEKPFVDGKLWCTDKLSGNFQTLRKVSGSTSETGGGNEQNIEKI
                     RNRLHPQTESMEGAGFYYVCGKLNAPAIQIRSVSNYVERRNKANWNIPLAVEQLNTFL
                     IQSLPSIHT"
     gene            10292..10825
                     /locus_tag="PLM90_01555"
     CDS             10292..10825
                     /locus_tag="PLM90_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HMZ88080.1"
                     /translation="MKADSIFEFKDSRDGKTYSAFTINGVAWMSENLRYNSKGSWCYE
                     SDGKSCKEFGRLYNWNDAILVCPNGWHLPNRAEWDSLIVWTGGELKAGHNLAYNDSLP
                     FNIVFGYPPNVNGRYSGGGIQASFWSADEYNSSTAWVYYFLKEKLPLTFSNYFSKNYG
                     MMCRCVKTIADSSSETN"
     gene            10952..12412
                     /locus_tag="PLM90_01560"
     CDS             10952..12412
                     /locus_tag="PLM90_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88081.1"
                     /translation="MKKMTGFLLGFLVWSLGVFAQTSGGLFSYGPDMNSTRIFPYVSQ
                     KPDGDVMVFGGRSNGFISSAYADLYSIETNTFTNLNMLYTHDNTVMVKLNDGRVLLAG
                     GGFDYGVPAHQYAEIYNPNDNSFTAAGTMAYARMQGSGALLTDGKVLIVGGWYSPEAA
                     ANAELYDPVLMTSSYAGGLLNPRSNALVLPCNDGGALVIGGYPTYGGTTFNAVEYYNR
                     ETSSFELFSDEIISGETGWTTTLDPFYRKTTDMYILENGKYLLMLYRSVPTLEYTLAL
                     FDPENKSFEKVNTDYPLMGAYSDGGIIDYALNKEESLVYMLGVDNESGSTGYCIVTTD
                     LSTGKTYQPDESYTMPSGEYLIFTTLSYIPETGMLLAPGVSTSNTDYFNATAKSFLIE
                     PELKVDIPIQEDLIVLQVYPNPVKDMLMLILPSNIGGQAIVDICDMRGQILYRTNAFE
                     GEKLVIPVNYLPTGNYFLRVRNGNCNLSASFIRAGN"
     gene            complement(12538..12608)
                     /locus_tag="PLM90_01565"
     tRNA            complement(12538..12608)
                     /locus_tag="PLM90_01565"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12574..12576),aa:Cys,seq:gca)
     gene            complement(12662..13009)
                     /gene="folB"
                     /locus_tag="PLM90_01570"
     CDS             complement(12662..13009)
                     /gene="folB"
                     /locus_tag="PLM90_01570"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HMZ88082.1"
                     /translation="MASIKLEGMRFRSFIGVYADEQAHGNDFLVDITITSEKITSTHD
                     QLEDTLDYVRVYRIAEGVMAERYLLLETAVQEIIKRIRELNFDINVVRVRVAKLNPPV
                     GGDMHSVSVEMEG"
     gene            13099..14277
                     /locus_tag="PLM90_01575"
     CDS             13099..14277
                     /locus_tag="PLM90_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ88083.1"
                     /translation="MPNDLFEGVDYYQLDDLLTTEHKLIRDTVREYVKKEVSPIIEEY
                     AQRAETPKHLIKGLAEIGAFGPTIPEEYGGGGLDNISYGLIMQEIERGDSGLRSLVSV
                     QGSLVMYPIYTFGSEEQRRKYLPKLGSGEMIGCFGLTEPDHGSNPGGMITNIKDMGDH
                     YLLNGAKMWISNAPFADIAVVWAKDETGKIRGLVVERGMEGFTTPETHGKWSLRASAT
                     GELVFDNVRVPKENIFPEIKGLKGPLMCLSTARYGISWGVIGAAMDCYDTALRYSKQR
                     VQFGKPIGGFQLTQKKLAEMVTEITKAQLLAWRLGVLKNEGKATPEQISMAKRNNVKM
                     AIDIAREARQILGGMGITGEYSIMRHMMNLESVITYEGTHDIHLLITGAHVTGLDAFK
                     "
     gene            14378..15343
                     /locus_tag="PLM90_01580"
     CDS             14378..15343
                     /locus_tag="PLM90_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HMZ88084.1"
                     /translation="MHPFLAKLLADKAKKEKKSEVLSNYILAREFAKFRLRVRRIVAA
                     VALLAFGVSAAAFGLLGFLLPNHFVDGGAVGVAMLSAQVSGLDFSVLLVVISIPFIIL
                     GYRTIGLQFAIRASIGIVALALAVEFIDIPIITEDKLLVAVFGGFFLGLGTGMTMRAG
                     GVIDGTEIMALQLSKKLGVTVGDVILIINIIIFSIAAWLLSLEIALYSTLTYLAAGRT
                     VDFILEGIEEYTGVTIISPKSEDIRLMITEKLRRGVTIYKGKRGFGSHGHRQAEIDIV
                     YTVITRLEINRLSAEIEQIDPEAFIVMQSVKDTRGGMIKKRAHKH"
     gene            15445..16026
                     /locus_tag="PLM90_01585"
     CDS             15445..16026
                     /locus_tag="PLM90_01585"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HMZ88085.1"
                     /translation="MRQIIVIALLLLTLSSRAQVMVDIPTMMQEEQLARDVYQLANEK
                     YQMHVFECKINAETNHWTMLRSYSETPNLEMPAYGSLSDSIFKAAFSDYQTMVTTSAT
                     EALKAGAMIEETDIFVLEVNRIKAEDEQLKALLDNLITASEQHLSSFLTALKADGVTY
                     TPSLLSEERIQRLMNEGNCKPGAACKGNSSCSH"
     gene            16229..17773
                     /gene="dnaB"
                     /locus_tag="PLM90_01590"
     CDS             16229..17773
                     /gene="dnaB"
                     /locus_tag="PLM90_01590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HMZ88086.1"
                     /translation="MAESDNSTSNFQKRNERRRPQADLSSVVYGKVPPQAGDLEEAVL
                     GAFMIDRDAVTNAIEILRADSFYDDRHRLIFTAIRRLFEKTQPIDLLTVTEELRRMGQ
                     LEEAGGAFYITSLTNRVASAAHIEYHARIVAQKFIQRELIRISSEIVHDAYEDTTDVF
                     ELLDKAEQNLFNIADKNLRTSFSDINNLLSMTIKEIEEARKHEDMLTGCPSGFSALDR
                     VTGGWQKSDLIIVAARPAMGKTAFTLSLARNAAVEFKKGVAVFSLEMSSQQIVKRLIS
                     AETEIPAEKIIKGNLAEHEMVQLVKLSGRLSEAPIFIDDTPAINIFELRAKCRRLKMQ
                     HDIQLVVIDYLQLMTAGDSSKGGNREQEISQISRSLKSIAKELNIPIIALSQLSRKVE
                     DRGGTKRPMLSDLRESGAIEQDADLVCFLYRPEYYGLDVDENGNPTKGIAEVIIAKHR
                     NGALENVRVRFVDKYIKFVDLDEMYTPQNLTPLANILPENKATITRKSKMDDLNDLDM
                     QEEPPF"
     gene            complement(17797..18852)
                     /locus_tag="PLM90_01595"
     CDS             complement(17797..18852)
                     /locus_tag="PLM90_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMZ88087.1"
                     /translation="MKKFIVSTLFFHLIIISIAIVIDLSEKSDKFGERNAPTAEVVQY
                     YIDFIPFIASILAPLLTFLAVIFFTSRLAYNSEIIAMLNSGMTFRRFLRPYFICGLFA
                     VGFLLYSNYWLVPNTNKRKLEFEDKYIHMPKNYGNNLHLKLDKNSIISLERFKFKSNE
                     GYNCSLEVFSGTGAKKTLSRKINASKIVYKENNTWQFVDYKAWYINGQSESYKEGRQM
                     DTILNLVPGDFDADIRVKDVLTYQEMEDYIKEKESEGTGELEYFRVEQYRRASSAISA
                     LILVIMGAALGSRKIRGGNWFNFIAGVALSAGYIFFLQFTSSFSINSSLHPIIGTNIP
                     NIVFALIAVFLVKRASK"
     gene            complement(18906..20072)
                     /gene="tgt"
                     /locus_tag="PLM90_01600"
     CDS             complement(18906..20072)
                     /gene="tgt"
                     /locus_tag="PLM90_01600"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMZ88088.1"
                     /translation="MHKLSTITYFCPVFFKLTAKDLHSSARAGELQTDHGSIPTPIFM
                     PVGTIGTVKGVHFQELEDDIQAAIILGNTYHLYLRPGIDTIEKAGGLHAFNSWKKPIL
                     TDSGGFQVHSLAQIRKIKEEGVTFQSHIDGSKHFFSPENVVDIQRSIGADIIMALDEC
                     TPYPCERSYAQKSMGLTHRWLDRGIAHFEATAPKYQHKQFFFPIVQGSVYPDLRKISA
                     EYIASKGAEGNAIGGLSVGEPHEDMYAMTDLVCNILPQDKPRYLMGVGTPANILECIA
                     LGIDMFDCVMPTRNGRNGMLFTADGIINIRNKKWEQDFSPIDINSTSSVSRRHSKAFL
                     RHLFASGERLGPQIASIHNLAFYLWLVREARMHIEAGTFAGWKNLMVQKVSSRL"
     gene            20143..21270
                     /locus_tag="PLM90_01605"
     CDS             20143..21270
                     /locus_tag="PLM90_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88089.1"
                     /translation="MGDSIILDSIFYLFCGAAAVQLFYILFFYLRLARYQQKSVMQDH
                     ALPPVSIIICARNEDSNLKKNLPRILSQDYPEFEVIVVDDNSEDSTTEYLFFLSQKEP
                     RLKRTSTRNSNRMMAGKKFPLTLGLKAASHEIVLLTDADCYPASDKWLREMMNGYGSG
                     AEIVLGYGAYEKQTGRLNRRIRYETVSSAINYLSFALAGLPYMGVGRNLSYKKHLFFN
                     SNGFFDHRHLPSGDDDLFINMVARKKNTAIIINNDAFTYSEPKETWDDWREQKTRHLS
                     TARYYRFKHKFWLGLQPITHILFWLSGIASLSLLYPWWYIVAGTIILRWLLLRYTLGK
                     SMQKLDEKDLKAYIEWYDILQIFYYFRFAKAALVKTKYRWD"
     gene            21261..21863
                     /locus_tag="PLM90_01610"
     CDS             21261..21863
                     /locus_tag="PLM90_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ88090.1"
                     /translation="MGLNDNLSDRAKEDLALVESARRGDQRAYGELMSRYKDTIHYMV
                     LKMVHNRDDAEDLTIEAFGKAFLHLERYTPDFAFSTWLFKIAVNNAIDFIRKKRLHTL
                     SLDDEQDEFTRSAFTNIRTNNPDPEEKFIKEQRANIMRTILDELNPKYKALVEMRYFD
                     ELSYEEISETLNIPLGTVKAQLFRARNLLAEILADRRAKM"
     gene            complement(22000..>22665)
                     /locus_tag="PLM90_01615"
     CDS             complement(22000..>22665)
                     /locus_tag="PLM90_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMZ88091.1"
                     /translation="MIVTGTGRVGKGAIEFLRKIGVREVTKEALLNETFSEAVFCVLD
                     SDDLYSRKSDGKYDRNEFHSHPELYHCTFSPYTKVCDVLINCIFWNPAAPSLFHKNEM
                     DATDWKIKVIADVSCDVKGGIPATIRSTTINEPVFGYDVATDMEIAPYIPESIDIMAI
                     DNLPNELPRSASEEFGQQLISTVWNELFQDNSRMLDEATICKNGDLNKPFEYLRDYVT
                     GES"
     assembly_gap    22666..22765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22766..23241)
                     /locus_tag="PLM90_01620"
     CDS             complement(<22766..23241)
                     /locus_tag="PLM90_01620"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMZ88092.1"
                     /translation="MQQFKVGIIREAKFPPDARVPLIPAQVQQLRQQFPNIEIVVQPS
                     KGRCYADYEYTDLDVTLQEDLHDCDILFGVKEVPYDLLIPGKTYFFFSHTIKKQPHNK
                     KLLQVLMQKNVRMIDYECLKNTEGNRVIAFGRWAGIIGAHNAIWTYAQRLGEQHLKR"
     gene            complement(23284..24576)
                     /locus_tag="PLM90_01625"
     CDS             complement(23284..24576)
                     /locus_tag="PLM90_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ88093.1"
                     /translation="MEGGISPRFRIFVHVKKAYLLVLVAIASVFQAEAQMQAHDTCRF
                     MTYNVLNFDYTGNFKENYLRTIVSEIHPDIMVVQELIDDSGAARFRDSVMLQVDTNYA
                     MAEFINSYDTDNGLYYRKDKYAFLSNTVVTTELRDINQFEMRHIASGIDFYVYSLHLK
                     ASSGGTNAAQRQREVDSLRKATLALPDTAHILVCGDFNIYGSYEEAYLHLLDPTDPGY
                     VIDIWQDSLTSTWNNIANTDYLTQSTRIAAFGGGSTGGMDDRFDMILMSPSLVDTAGM
                     GYIPGSMYPVGNDGNRFDNEINDPVNTSVSEEMADALYFTSDHIPMFAEFSFSYIIPE
                     EPEDTTDTLIQQIAETDLLLQLYPNPAGDVITATSNFNAEGYIIYGADGKTVYKMTEK
                     SIPAGISEKIAIADLAPGLYVFVSFNGIKRVQSTFLKN"
     gene            24597..27206
                     /locus_tag="PLM90_01630"
     CDS             24597..27206
                     /locus_tag="PLM90_01630"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMZ88094.1"
                     /translation="MEISKTYDPASIEKKWYAHWLANRYFHSKPDNREPFTIVIPPPN
                     VTGVLHMGHVLNNTIQDVLIRRARMQGKNACWVPGTDHASIATEAKVVKMLREQGIKK
                     SSLSREDFLKHAWEWKEKYGGIILQQLKELGCSCDWDRTRFTMDDLYYDDVIDTFIDL
                     YNKGYIYRGLRMVNWDPEARTALSNEEVIYQDVTSRLHYVKYQIKGDNVYLTVATTRP
                     ETILGDTAICVHPDDERYKEFIGKTVLVPLIKREIPVIADTYVDMEFGTGALKITPAH
                     DPNDYEIGLRYNLPVIDIMNEDGTLSEKAEILIGEDRMVARKKMVAMLEEEGYLVKIE
                     ELKNSVGFSERTNAMIEPRLSMQWFISMKEISKPALEHVMNGDINLHPEKFINTYRHW
                     MENVKDWCISRQLWWGQRIPAWYNASGEFVVAKTEAEARAIFTSKGMSLDGFRQDEDV
                     LDTWASSWLWPISVFNGMKDPKSADIEYYYPTQVLVTAPEILFFWVARMIIAGYEYRN
                     EKPFSDVYLTGIVRDKLRRKMSKSLGNSPDPLDLIAQYGADAIRMGILLCSPAGNDII
                     YDDKLIEQGRNFNNKLWNVLRLIKGWEVTETSQTKNEAAIQWFDSKLNDTINTINDHF
                     EQFRISDALMCLYNFIWDDFCSWYLEFIKPEFGQPIDSITYEKTIDFFEGCLKLLHPY
                     MPFITEEIWHLLRDRKAGDDIVITQLNDAGNINNNILASGNEAKELISRIRDIRAKNN
                     VSPKVAASLYAEGDITSMQAFRAVIMKMANLEVFESVTGDVKNAVTFLVGTQKFFVVV
                     QMEINIEEERKKLEEQIAYQKGFLLSVDKKLSNEKFVGSAPAAVVDLERRKKADAEEK
                     IRILEESLRNLGN"
     gene            27253..27804
                     /locus_tag="PLM90_01635"
     CDS             27253..27804
                     /locus_tag="PLM90_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88095.1"
                     /translation="MKKGFLVILAIASMATLNSCGKKKGPSAETKQAMATFESEWKAL
                     GDQIAAWETTMNTAMDGMTQMMTATRSMDITKLSGESKSMAEMNLMMCSKIDEQMVAM
                     KTGFAKAKTDFAAATDEYGAWKKKGQEEHLDDATITSELANWTTKLTEWKTTVEGLNQ
                     QLSGMQDACKQSCDAITAMVPAK"
     gene            28013..30409
                     /locus_tag="PLM90_01640"
     CDS             28013..30409
                     /locus_tag="PLM90_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88096.1"
                     /translation="MRNFLLICLLLSTISLTAQSPGQTVRGTITDAASKYPLIGATVL
                     LYADSTMLGGATTDEQGDYRITNAPVGRVTLRFTYVGYEDITMSNVIVTGGKEVIINV
                     ELEEGAEALDAVVVTAKSDKGEVNNEMAINSVRTFSVEETDRYAGSRGDPARMASNFA
                     GVQGADDSRNDIVVRGNSPIGVLWRLEGIDIPNPSHFAIAGTTGGPVSIINNKYLSNS
                     DFFTGAFPAEYGNAIAGVFDLNMRRGNDEQHEFSGQLGFLGTELFAEGPINKSKGSSY
                     LASYRYSTLQLFQFMHIPIGTTAVPNYMDGAFKLSFPKGNAMWNVFAIGGKSKIDILV
                     SEYTKEETELYGDSDRDQYFGTAMSVAGVSYARQYNPSAYGKWVAAYSVQENHANHAF
                     VFRDVNFDIDSIVPNMNYSFLDQKASLIYSLNKKVNSRHSFKTGASFESIWYNHIDSN
                     RNVYTWTWDNRYDFEGNNYLAQAYFQWKYKYNERLLFTGGLHAQIFTINSNSKSLEPR
                     AGMQWRVDDRSSLSLGLGLHSQMLPSYQYTYMHYQADGSALLINEDVGFIRSAHMILG
                     YDRKLSDNLRVKSEVYYQYLYDVPVEVAPSAFSLANEGSGFSRFFPDSLQNTGTGKNY
                     GAELTIEKFFSNHFFFLITGSLYDSRYVGSDGIERKTDFNGTYAGNMLGGYEFTISDR
                     NVLTVGIKTTYAGGKLTTPVDTAATIAAKELIYIDSLTNTIQLKDYFRFDVKLAYRIN
                     SGKRSVTHEIALDLVNVLNIQNVLGLTYAPNPADPTASPVKEEYQLGFLPLFYYKIDF
                     "
     gene            30413..31195
                     /locus_tag="PLM90_01645"
     CDS             30413..31195
                     /locus_tag="PLM90_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ88097.1"
                     /translation="MTYSTILYTIADKVGTITLNRPDKRNAFNADVVAELTEAFTDAQ
                     ENESVKVIVLAASGEVFSAGADLGYLQSLQQNSFEENVADSQKLMALYKLMYTLSKPI
                     IAKIEGHAIAGGCGLASVCDFSFAVPEAQFGYTEVKIGFIPAIVMVFLLRKINERHAK
                     ELLLTGKLITAETAEKYGLINAVIPKNKIDEAVSGLCVELIAQTSRVAVATTKSMIAA
                     VQEMGLDEALQYAAEMNAKARATDDCQYGIRSFLEKKKPEWK"
     gene            complement(31196..32491)
                     /locus_tag="PLM90_01650"
     CDS             complement(31196..32491)
                     /locus_tag="PLM90_01650"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HMZ88098.1"
                     /translation="MRDDGPIWYPFTQMQLAPEPVQIVSGKGALLFTADGNTIIDAVS
                     SWWTNIHGHAHPYIRQKVHEQFGQLEHVIFAGFTHAPAMHLASRVTAMCGSDYTKMFF
                     SDNGSTAVEVALKMALQYHQNLGAKKHRIIALEGSYHGDTFGSMSVSARGAFTAAFHD
                     KLFDVTYITPPLPGNEDICMQELEAALSEGDVAAFIYEPLIMGAGGMLMYTGMVLDTM
                     IYTAKQHGVLCIADEVMTGFYRTGKFLAGHYANHQPDIICLSKGITGGSMALGVTICT
                     QAIFMAFYSEDKMKALYHGHSYTANPLACAAANASVDLCLQPGFDHALTTIASRHQHF
                     TAALQVYSHSDIPVVKNIRQCGTVLAMDFVTSNDTGYFNSIRDMLYHFFMKRGVLLRP
                     LGNTIYIMPPYCITNEALDEVYNAILDALALLAEGKTLQ"
     gene            complement(32493..33125)
                     /gene="bioD"
                     /locus_tag="PLM90_01655"
     CDS             complement(32493..33125)
                     /gene="bioD"
                     /locus_tag="PLM90_01655"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HMZ88099.1"
                     /translation="MKKIFITGIGTDIGKTVVSAVVVEALFADYWKPVQSGGLDYTDT
                     QRVQNLISNPKSQFHKEAFLLKQPMSPHAAAALDGVEIDLDNIVVPETSNTLVIEGAG
                     GLMVPINNSTLMLDMIAKWEASVIVVSKNYLGSINHTLLTCAVLRAKQIPVAGIIFNG
                     MENVSSEQFITQYSDIPVIGRVAHTDVLDKPFIKKHAEFIRKRMQQLKIS"
     gene            complement(33398..34327)
                     /locus_tag="PLM90_01660"
     CDS             complement(33398..34327)
                     /locus_tag="PLM90_01660"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HMZ88100.1"
                     /translation="MTPVFAKAYSPSEVHFQIEDCDNRNEPRKVMMCTPDYYNVVDVK
                     NVYMQHQKGNINKERANIQWDNIRNSYLALKNQKFLDDVVVINGVEGMEDMVFCANQS
                     FPWVMWNGEKIAIMSRMRHENRQNEIPYIEDFYQDHGYKIVELQSKYHFEGMGDLIAH
                     PGKRVLYGAYGFRSEKAVYDEISRIVDAPIITLELINEHHYHLDTCFLPLNHTEVLIA
                     PDAFTPESLAIIRRMFEGVYEIPEDEAIDGFACNAHIIYGTNKNSSAAVIQRGNPVSL
                     NIIQQSANPVIETDTTEFMKSGGSVFCLKMMFY"
     gene            complement(34400..35287)
                     /locus_tag="PLM90_01665"
     CDS             complement(34400..35287)
                     /locus_tag="PLM90_01665"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HMZ88101.1"
                     /translation="MKNVTVIGAGTMGNGIAHVFAMKGHKVTLVDVSEPALQKAVATI
                     TNNLDRMVAKEKITADEKAATLSNITTYANFDHSTNADLVVEAATERVDLKLKIFTEL
                     DQICAAHTILASNTSSISITKIAAATKRADRVIGMHFMNPVPVMKLVEVIRGYATSDE
                     TAKTIMELSAALDKVPVEVNDYPGFVANRILMPMINEAIYTLYEGVAGVYEIDTVMKL
                     GMAHPMGPLQLADFIGLDVCLSILNVLHDGFGNPKYAPCPLLVNMVTAGYLGAKSGEG
                     FYAYTAGNKDLVVSPRFNK"
     gene            complement(35311..>36293)
                     /locus_tag="PLM90_01670"
     CDS             complement(35311..>36293)
                     /locus_tag="PLM90_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88102.1"
                     /translation="LHIQQYLSHATHYGNNVPLTVYFFDYDFNLFDSVDVVMSGDRQL
                     IIVGIPNPGSWGYTVLDYRNKLNDAVTSDEVHFTEPGTYSLDNAMMDIEITSVTEPGF
                     IYAQHFWAAPNNYIAAHPGLHVSTDRFWRITTNIPVTGDATAKFLYNGQATIGSGYLD
                     NDFITNSEDSLVLLYRNGPQGDWIIYPDYTLNTWGSANDKRGAFELSAVTSGDFCIGI
                     YDASIPDQPLYIPETDCSFNGAIENLSDNSLTVYPIPANQLIVATVDQQLMGMQLSLY
                     DTSMRKIWTGRIDNLSMSIPTQNLPAGTYQLCVENARSERIAAEKVLIMH"
BASE COUNT        10591 a         7285 c         7781 g        10536 t
ORIGIN      
        1 tagcattatc cctggcagtg ataagtcttc tattaggtgc ctgtaagtca aaattggaga
       61 agaaagacca atcattagac gagatgcagg aagatgcagg taagcaggaa aactatgttc
      121 cggttcttga gagtgccgat gcaacacctg tttacaagaa gatcggagat tctgttgaaa
      181 tgcatctgaa tatttactat cctaaaaaac atgacaaaca agctgcaaca cctgtaatta
      241 tttacttctt tggcggcgca ttcattcatg gtagcccggg acaatacgaa gaccattgta
      301 aatattttgc aagccgcgga attgtcgcca taactgctga ctaccgtgta atttccagaa
      361 acaaaggcaa tgccattaat tgtgttgttg atgcaaagag cgcagttcgg tatgtgcgtg
      421 aacatgcgaa ggaattgaac atagatccga ataaagtaat tgtctccggt ggttctgccg
      481 gagcattttt agccttgact tgtaatatga atgatccgaa atggcaagat tcaacagaca
      541 acgctcaagt gtcatgtgca ccaaatgcga tgatactgct caatcctgtg gtgaattcta
      601 tggagcataa atttcgtatt caaaaattta aggatgatgc taagcaacct gatagcgaat
      661 cgcatgcagc cgaaatcaat ccattgacat cgataagagc aggaatgcca cctaccataa
      721 tttttcatgg aacaaaagat aaaatttcag gatatcagtt tgtaaaacag tttgccgatg
      781 actatgaaaa agccggtaat cgctgtgagt tggttactta tgaaggacaa aaacatggat
      841 ttactgccat taaatttgaa gacggaaaat attatcgcga tgcgctaaaa aaatcggatg
      901 agtttctcat ttcactgggc tatttgagcg gtaaacccac ggttgaataa aacttgaaaa
      961 gccggcgctc aatgccggct ttttttagca ttaacatgaa ttaacttttg caattgcgat
     1021 cttaagcttc gcaacgtgta ttattgtatg aaaacccacc ggatgcgaat ttttgttagc
     1081 ctgtttattt ctggtttact tgttacaagt gtaaatgcac aaaccattat taagcgtgaa
     1141 cacagcgctg tacctatcaa tgaaaatata catatcgatg gtgtgttgaa tgaggcatca
     1201 tggaaccagg caccaattgc cgataatttc actcaatttc aatttaaata caatatggcc
     1261 tctgcctata aaactgaggt gcgcgtattg tacgataaca ccgcaattta catcggcgca
     1321 accatgtatg atcctgcgcc ggatagcatt caagtgggta tgacacagcg cgatgaattc
     1381 gcgacggccg atttttttgg gataattctt gacccctacc ttgatggtaa taatgccctt
     1441 gaattttttg tttctgccgc aggcgtgcaa ggcgatgcca aagcttatgc aggtgcagga
     1501 aattttggtg aggattttaa ttgggatgcc gtttgggcca gtgatgttaa gatttctgaa
     1561 ataggatggg ttgctgaaat gcgtattcca tattctgcga taagatttcc aaatgtggat
     1621 gtacaaacct ggggtgttaa ttatatccgg gaagtgaaac gaaatcgcga taagagttct
     1681 tggaataatt tcgatcctca gattgatggc tttgtgaaac aatgcgggtt gttgaaaggc
     1741 atcgaacata ttgaacctcc aattcgcttg tcaatttcac cttatttttc gtcatactat
     1801 gatttgtaca gcgacaaaat aaatgacatc agctcaacag catggtcgtt taatggcggg
     1861 gcagatttga agtatggatt gaatgacgca tttacacttg atatgacctt gatccctgat
     1921 ttcggtcaag tgcaaagtga taaccaggtg cttaacctca caccattcga aacatactat
     1981 gtagaacagc gacagttttt taccgaggga actgagttgt ttaataaagg cgatctgttt
     2041 tatacaagaa gaattggagg tactccatat ggatattatg atgtgtacta cggacttgat
     2101 accacggaat ctgtaatcga taatcccatt caagctcact tattaaatgc ggcaaaagtt
     2161 tccggtagaa caagcaaagg tttgggaata ggtgtattta atgccgttga aggtgaaacg
     2221 tacgcaacga ttgaaaatag cgaaggtgaa aaacgcactg aacttacagg cccgcgtgca
     2281 aattacaatg tattggtttt cgatcagcaa ttaaaaaata actcttacgt aagcctcatc
     2341 aataccaata ccttgcgtga tggcaattat caggatgcca atgttaccgg catggaattt
     2401 ctggtaaata ataagtctaa tatttggggt gttgaaggag gcgctgcata tagcagtatt
     2461 tttgatgctg atgcagaacc taccacaggt attcatcata gaatccgtgg cggtaaaacc
     2521 ggtggtaagc gcagatttat tctcgggtac gattatatcg gatcaacata caatataaat
     2581 gatctcggtt atcttcctat caacaatcaa ttgaattttt catttaatac aagctataat
     2641 agctacgaac cgctatggca tctcaatcag atacatatgg tgtttgaaac aaattatctg
     2701 cgtctggtag atccggaagc atttcaaaat ttcagcattt acggagagac ctatggaagc
     2761 tttagaaatt acatgtacgc cggagttttc ttttacaccg agcctgtcac aacatacgac
     2821 tactttgaac ctcgcgtgcc cggtagattc tatacttatc ctgtgaatta taatttcgga
     2881 ggatggcttt ctactgatta tggcaagcgt tgggcgatag acatcaacag caactatcgt
     2941 gtatttgatg atgaaggtcg atatcgctgg aatgtgagca tgtcgcccag atggcgtgct
     3001 acagacagaa ttttaatcat attggatgcg ggtcgatatt actggcccgg tgatgtgggt
     3061 tttgtgaatt taatgggtga cagtattatc atgggaagaa gaaaaaatgt cactatggaa
     3121 ggcgtattga gtgtaaatta cgcaccatta gtcagcatga attttaatat gcgcttgcgc
     3181 cattactgga gttttgccga atacagtgat ttttacaatc tcggtgaaaa tggagattta
     3241 ttggctacaa cttataattc atttcttccg gatggcacaa gttccgatga cgtcaatttt
     3301 aatgccttta atgtcgattt ggtatatacc tggtatttcg caccgggaag tgaattaaat
     3361 gtggtgtgga aaaatgctat ttatcaattt ggaacattat tacctgagaa ctatgtagat
     3421 gaccttaatc aggtgttcga ttcgcctacc aataataact tctcaattaa ggtgctgtat
     3481 tacctcgatt acctctatct gcaaaagcga aaagcttaag ctaactttgt gctatgctta
     3541 ggcatttatt atcgcttgct gtatgcttat taggttttgg tattattaat gcccaaacac
     3601 cggcaattga tgtgcatttc ggcgatatac attcaatcaa tcgaggctct tcctctgtat
     3661 tggatgatgc aggcggcgtt tggtttgcag gaaccatttc gaatggtgca gagggatcag
     3721 atgacatagc catttatcgt atcaaacaat ccggcgaaat tctttggtgg catgcttatg
     3781 gcgatgccag aaacgaatat gtaaatacga tcgctgccgg aggtgatgga attttgtatg
     3841 catgcggtga tgtccgcgat cctgaaacgg aagatttaaa tgcattcatc atggctttag
     3901 acacggcagg aaatgtaatt tggaatcagg aatatggaga ggcagccacg aatgaggaat
     3961 tctatcaatt agctattgcc cctgacgcaa cgattttgtt aacaggattt cattccgctc
     4021 tcacaggcac aggaaatgat gtattgctcg caagatatgc tcctgatggc gccttgttgg
     4081 aggatactgt attcggcacc gatagaaatg aagtgggtat gagtattgtt gcgcttcccg
     4141 gaggaaatat ttttgtctcc ggtgacaggc aaacaattga tggatattat aatccgatct
     4201 tcattaaatt atcaccagat tttaatgtca ttaatgatat tgatttgact tcgccacata
     4261 atagcggctg taaatctatg caccttaatt ttcagggtga tttatatatt tcaggagaaa
     4321 cagcatcttt cgccacacct gaatttgatg tgttatttat tgtggttgat acgcttggaa
     4381 atctggtaac tcaaaccggc attccttcgg ctggccccga tgcaggatac gacatcgtgc
     4441 agctgcctga ttcaacatat gcggttacag gatttgctta caatccggcg aaaggtgaaa
     4501 atgatatgtt attaatgcat atcgattctt ccgggaatga aatagaccgt cttttttttg
     4561 gtgaaaccgg tgcagatatg ggatatgata tcaaagttga tgctattggt aattgccggc
     4621 tatccggttt tcataccaat gctgatacga catttttctc attggtgatt gcaggttttc
     4681 ctgcgcaaac ggagactgaa aatatagcct cgcccgaaag gttcttgtca ataaatccaa
     4741 atccaataac cgaatctatt tgtgtgcaaa ctgagttgga agttgaccgt gtaattgttt
     4801 atgacaactt agggagaaga gtaggggatt tgcctaaaac atcagggcaa tgttttgaat
     4861 ggaatacttt attgccaaaa ggcatttatt atatacagat gtatacatct gtaggtaatt
     4921 tcactaaaaa aataatagcc ggatgatcag tatcacccgg ctatcaaaat ctatattcag
     4981 ttagttattg aatattcatt tttttcttca ccagtggaag aatatcatcg ccttcaggtg
     5041 ccataatgac taccgacaaa gaattatcta tcacatgtgt gtagccgttt tctttggcaa
     5101 cagttttaat gatttcagta gtgtttttga tgataggttc aaaaagttcc tgctttttag
     5161 catcaagctt ttgctgtgtt tctttctcgt atgaagtaat gcggtcttca agcattacgg
     5221 catcctggat ttttgcttca cgagcaactt cactcaggtt tgggttcccc tgtatatcat
     5281 tcaccaacat ttgatattcc tgcacataat cctgatatag agaatcgaat tcacgagcat
     5341 atgcaacaag cttgtcgtta gccgcttttg cctcaggcat tgacgaaagt aattcctgtg
     5401 agttgatgta tgcgattttt acctgctgag ctgaagctat tgagctataa gccattacag
     5461 ctactaccag catccatttg atttgtttca tgatataata acagtttatt tataaaaatt
     5521 gagcgccaaa gatacagaaa cggttctgcc cctgcaatca tctgtcggcc atacctagca
     5581 ccactaaagc tgcaaccgga acaggaacat acccttcaac cacccattct tccgcatcat
     5641 tgaacctcca catcggtcgc caaacgcctt catcaaaggt ccactgaaga tcaggattgc
     5701 tgtcccagta aggcttcaac atagcatcat cccacaacca ttgcttgtcg acacccgatt
     5761 cccaaaacgg tttcagcacc tttccatccc aaacccactc cgtctgcgga tcagcgttcc
     5821 atgcgggttt aaggtacttt ccgtcaaaga cccattcatc ttccggccga tagccccaca
     5881 atggttttaa cgtctggcca tcccaaaccc actcataata gggtcccata tccaatttag
     5941 gatgaatgaa gcttttcccg tctgtgacaa tatctaccga aaactgtgcc attcttgccc
     6001 tgacactgtc gtcgagcgaa aaatgacgaa tatattgatg tgcaacagca gtgacttgcg
     6061 attgcgaagt caatttcccc tcaattacac caatcaagag atcgcttatt ccactgtata
     6121 cttctgtgag cgaatcgtta attacttcaa aatgcaacag ttcttcattg tctttgtcga
     6181 tgggatgatc gccgaggtag aaactatttt gcatggcgat atattgaacc gtgctgccat
     6241 cggcttgtaa aatgacaccg gatttgcgat gtaacatgtc ctttgcattc accagaaata
     6301 atatctgttc cttcgaagtg ctatcaccaa cataaacagt atttccactg agtgtatacg
     6361 ccacttccga agggccggta aaaagtttat tgtcggaaat ttttccgatt attgtattgg
     6421 aatcaataaa cagataaata tccgttgatt gtgcaaacga tatgctgttg aagcatatga
     6481 ggatagtgca tattccggaa ataatgttac gcataaatgc tgattaaagg tacgcaattt
     6541 cgaacgatat ggctgcatcc ggatgatgag aactgtgtgc aattgatcga ccaacgatgg
     6601 cttccgcatg aggttcgtat tgctgatatt cgcacagttg atgaaatgtg cactgccatt
     6661 gccgacatgt gggtgcgtgg tgctcctttg ataggtgccg ctgctgcctg gggcatgtat
     6721 cttgctgcat gcgaagtgcc cgccagttgt gccagagatt tcttttttga agaggcatat
     6781 ggcaaattat taaaaacgag gcctacagca gtaaatttaa aatgggcttt ggatgaaatg
     6841 ctgactgcga ttaccggtaa tgattcaata atcgaatcaa aatggtatgc acgaaatcgc
     6901 gctaaatgga ttgcagatga tgatgtaaaa gtgaacactg ctattggtga atatggttta
     6961 tcattgttat cggctattgc agaaaaaaaa tccggtgaac cggtacaaat tcttacacat
     7021 tgtaatgccg gctggcttgc aacagttgac agaggaacga taactgctcc gatttatttg
     7081 gctgcggaaa aaaatattcc cattcacgta tatgtaagtg aaactcgtcc gcgtaatcag
     7141 ggcgcgcaac tgacagcatg ggaattgggt gaaaataata tcccgcatac tttgattgca
     7201 gataatgccg ccgggtatct aatgcagcaa ggtaaaattg atatcgtatt agttggtacc
     7261 gacagaacat ctgcacgcgg tgatgtgatc aataaaatag gtacatattt gaaagcattg
     7321 gccgcgcatg aacatcaggt tccattcttt gttgcagcac cttcatcaag tattgatttt
     7381 accatgaaag atgcttttac agaagcaaaa atcgaagagc gtaacgccga tgaagttacc
     7441 tatgttcaag gaagagcaga tggcaaaatt atttcagtgc gcatatcacc tgaatctaca
     7501 actgccataa atcctgcatt tgatattaca cctgccaaat atgtcacagg cttgatcact
     7561 gaaaaaggaa tttgtagcgc ctcatttgaa gggctcaaaa aattatttcc tgagaaattt
     7621 agtttttgat cattccattt ttacagtaac caactcaatt attggcccaa ccatacaaat
     7681 agacacactg tttacttcag tataagtaaa tgatatgggt gcataatcca ccagttctaa
     7741 accaaagtct ccgagattta ttggttccat tgcctgattg tcggcattga taattaccca
     7801 tccacaacca tctaacccgg tatagtttct gagatatcct gtatccaatt gcaacgcatt
     7861 tgcaggtgga tccgtttcgc aggaactaaa tagggcagtc gctataatca agcctatcag
     7921 atatcttgtc attgtgaaaa tttgatactc aaagttagtc tcaaataggg tgtttcacaa
     7981 gtattttatt tcgaattaaa gcttcattcc attacttttg acgagatcat gacgcatgac
     8041 ggttatataa aatatgagca gcattgggaa atagctaagc ctttgacaga tatcgaaccg
     8101 ctggttttga tgcgtaatca attggctgca ttgcaatgga ttggggtgta tcctgatggt
     8161 gtcgggtatg gaaatatcag tttgagactt gagggcgccg accaattctt aattagcgga
     8221 acgcagaccg gaaacatgct aattgcagat gcatcgcatt tttgtatggt tgaaaaggct
     8281 gatatcaaca aaaatatctt gtggtgcaaa ggaccgataa aagcttcatc agaggcgctg
     8341 acacatgcgg cgttttatca gagcgatcgc aacatttgtg cagttataca tatacatgat
     8401 gcagccgcat ggaaaagatt attgtttgta aatccgacag taagtaaaga aattgaatat
     8461 ggaactccgg aaatggcttt tgctatgcaa gcgctcattc attcgcaaca aaataaattg
     8521 tgtggagtga ttgtaaccgc cggacatgca gatggcattt ttgcctatgg tgattcgctg
     8581 aatagcgcat tttcggtatt agaatccgcc atgcatcact caccaacaaa taatggataa
     8641 cgcaaactgc caataatttt tttctccagc gcaacatgat atagttgttc aacagcagat
     8701 ttgccttcaa ctcccaggtc gacactgaaa tcgttgacat ataatgcaat atgttgttgc
     8761 atcacttcag gcgacatttc ctgtgcatgg gcacaaacat aatccttcga catttcggga
     8821 tgtgccaacg cataggcaac gcttctttgt atgacgcgat taaccttttc ctgaatttct
     8881 attggaagct cacgacttac ggcaattcca cctaatggaa taggcatatg atattttgat
     8941 tcccaccatt cccctaaatc aactattttt cgaagtcctt tttgttgata ggtaaatctg
     9001 ttttcatgta tgatcaaacc aatatcgatg ccgcccatca acaaggattg ttctatgtct
     9061 gaaaatacca tctccttctt cgttgctgct tccggaaatg caagtgtaca taggaagttg
     9121 gcagttgtaa gtcgacccgg aattcctatc aaacaaaatt caatttttga ctttggaatt
     9181 tcatctttcg aaatcaataa tgggccgcaa cctcttccta gtgctgctcc tgatgtaagc
     9241 aacttatatt ggtgactcac ataggcaaag gcatgaaagc tcaatttact tacgtgatgt
     9301 ttttcttcca gtgcttgttg attgagtgat tcaacatcag caaggtggat gtcaaatgtg
     9361 agtccttcgg tgtctattcg cccatgtgca atagcatcga acataaaggt gtcgttggga
     9421 catggggaat atgcaagcga taatttcatg tatgtattga aggcaatgat tgtattaaaa
     9481 aagtgttgag ttgttcaaca gcaagcggaa tattccagtt cgctttattt cgtctttcaa
     9541 cataatttga gacacttctg atctgtatag ccggtgcatt caattttccg cagacataat
     9601 aaaatcctgc tccttccatg ctttcagttt gtggatgaag tcgatttctt attttctcga
     9661 tgttttgctc atttccaccc ccggtttctg aagtgctccc tgaaactttg cgcagtgtct
     9721 gaaagttacc tgaaagttta tcagtgcacc acaatttacc gtcaacaaag ggtttttcat
     9781 tgtgtgctgt tagtccaagg tcaaatacat caatgaagtc atcaccatcc tcagctccca
     9841 actgagcgaa gcaatcactc tgaacgagca ccacctctcc tttgctgaga ctagggtcaa
     9901 atgcgccgca aatgcctact tgaatggcga ggtcgaactt tttcttcata agcatttcgg
     9961 tcaggtgaaa cacggtagca gcagttccga cacctgcaat taaaatttcg atatccggga
    10021 atcccggcac caccaatttg aaggaatttg accctgtgga agtcgttgaa cccttgtcaa
    10081 tcaaaggttt tagctcaaat tgagtggctg ctgtaagcaa gatgctcata ctgcgaaggt
    10141 aagctgattt cctgttttcg tggttttcaa ggctctgaaa tcaatggaaa ttgcgaactt
    10201 tgccgggtta ttagcacata ttaccatgaa atattacatt atacttatct gcatatcttt
    10261 ttcatttgtt tcccttaaag cacaagggca gatgaaagcg gattcaattt tcgaatttaa
    10321 ggatagtcgt gatggcaaaa cttattcagc cttcaccatc aatggtgttg cctggatgag
    10381 cgaaaatctg cgttacaatt cgaaaggcag ttggtgctac gaatctgacg gcaaatcttg
    10441 taaggaattt ggacgcttat acaattggaa tgatgcaata cttgtttgtc ccaacggatg
    10501 gcacttgcca aacagggctg aatgggattc tcttatcgta tggacaggtg gagagttaaa
    10561 agccggtcat aatttagcat ataacgacag tcttcccttc aatatcgttt ttggctatcc
    10621 gccgaatgta aacggacgat attccggtgg cggcatacag gcgagtttct ggtcggccga
    10681 cgaatataac tcctccactg catgggtgta ttatttttta aaggaaaaat tgccgcttac
    10741 tttcagcaat tacttctcta aaaactatgg catgatgtgt cgatgcgtaa aaaccatagc
    10801 ggattcttct tcagaaacta attaattatt gtttttatct gttgacaaat atcagatatc
    10861 acaatagtga tatgtgttta ctatgtgatt gacagaggtc atactactta cgcaaagttt
    10921 aggtgacttt tgattagcta aactatgagt tatgaaaaag atgacaggat ttctcctcgg
    10981 atttctggta tggagtttag gagtatttgc tcaaacaagt ggtggtttgt tttcctacgg
    11041 acctgacatg aattctacac gcattttccc ttacgtttca caaaagcctg atggagatgt
    11101 catggttttt ggcggcaggt caaacggatt tataagttct gcatatgccg atttatatag
    11161 catagaaacc aatacattca ctaatcttaa tatgttatat acgcatgata atacggtcat
    11221 ggtgaaattg aatgatggtc gtgtattgct ggcaggtggc ggttttgatt atggggtgcc
    11281 tgcgcatcag tatgcagaaa tttacaatcc taatgataat tcttttactg cagctggaac
    11341 tatggcttat gcacgaatgc aaggttcagg agcattgctt actgatggca aagtgttgat
    11401 cgtaggtggt tggtatagtc cggaggctgc tgccaacgca gagttgtatg accctgtatt
    11461 aatgacctca tcatatgccg gtgggttact taaccctcga tccaatgcat tggttttgcc
    11521 ttgcaatgat ggtggagcgc ttgtaattgg agggtatcca acttatggcg gcacaacatt
    11581 taatgccgta gaatattata atcgggagac aagttcattc gaattatttt ctgatgaaat
    11641 tatttccgga gaaaccggct ggactactac gcttgatcct ttctacagga aaacaacaga
    11701 tatgtatatc ttggaaaatg gaaagtattt gttgatgctc tatagaagtg ttccaacatt
    11761 ggaatacact ttggcactgt ttgaccctga aaataaatct tttgagaaag tgaatacaga
    11821 ttacccacta atgggtgcgt acagcgatgg tggaattatt gattatgcgt tgaataaaga
    11881 agagtcattg gtatatatgc tgggtgtgga taatgaatca ggcagtaccg gatattgcat
    11941 agtgacaact gatttatcaa caggaaaaac ttatcaacct gatgaatcct ataccatgcc
    12001 ttccggcgaa tatttaatat tcaccacact ttcatatatc cctgaaaccg gaatgttact
    12061 tgctccgggt gtttcaactt caaataccga ttattttaat gctacagcta agagttttct
    12121 tattgagcct gaattaaaag ttgatattcc tatacaagaa gatcttattg ttttacaagt
    12181 atatccaaat ccggttaaag acatgctgat gctcatactt ccttcaaata tcggaggtca
    12241 ggcaatagtg gatatctgtg atatgcgtgg acaaatatta tacagaacaa atgccttcga
    12301 aggagaaaaa ttagtaatac ctgttaatta cctgccaacc ggaaattatt tcctgcgagt
    12361 aagaaatgga aattgtaacc tatcggcaag ttttatacgg gcgggaaatt aatgttttga
    12421 tgtatagagg cacttttatt cgctagctgc acacagcgag gagaattgcg ttcttcattt
    12481 gactgagaag tcaacaaaca aaaaaaccga cgttattgaa aacgccggtt ttttttgagg
    12541 tcggaagcgg attcgaaccg ctgtaaaagc ttttgcagag ccttgcctag ccactcggcc
    12601 atccgaccat gagcggcaaa attacacaga aaattgagat tccataatga ttgaacccct
    12661 ttcaaccctc catttcaaca gaaacactat gcatgtcgcc acctacagga ggattgagtt
    12721 tggcaacccg cacacgaaca acattaatat caaaattcag ttccctgatg cgctttataa
    12781 tttcctgcac tgccgtttcc aacaataaat agcgctcggc catcacaccc tcagcaatgc
    12841 ggtaaacgcg cacgtaatcc agtgtatcct ccaattgatc atgcgtgctg gttatctttt
    12901 cactggtaat ggtaatatct actaaaaaat cattcccgtg cgcctgctca tcggcataaa
    12961 cgccaataaa tgagcgaaac cgcataccct ccaatttaat gcttgccatc ctgtaaaagt
    13021 agtcatttgc aatctttcaa gcccccatga atattgtgta tgcagccaaa tccgtaactt
    13081 tgcggaaatt ttcactttat gcccaatgat ctttttgaag gagtggatta ttaccaactc
    13141 gatgatttgc tgactaccga acataaactc attcgcgata ctgttcggga atatgtaaaa
    13201 aaagaagtat cacccatcat tgaagaatac gctcaacgtg ccgaaacacc taagcatctg
    13261 atcaaaggcc ttgccgagat aggcgcattt ggtcccacaa ttcctgagga atacggcggc
    13321 ggcggtctcg ataacatttc ctacggattg atcatgcaag agatagagcg tggcgacagc
    13381 ggtctgcgct ccttagtaag cgttcaggga agcctggtga tgtatcctat ctacaccttc
    13441 ggatctgaag agcaaaggcg caaatacctc cctaaattgg gtagcggtga aatgatagga
    13501 tgttttggac ttacagagcc ggatcatgga tctaaccccg gcggcatgat caccaatatc
    13561 aaggatatgg gtgaccatta tctgctcaat ggcgctaaaa tgtggatttc aaatgcgccg
    13621 tttgctgata tcgccgttgt ttgggctaag gatgaaacag gtaagatcag aggacttgtg
    13681 gttgaacgag gcatggaagg atttacaacg ccggaaactc acggaaaatg gagtttgcgt
    13741 gcatctgcta caggcgaatt ggtgttcgat aacgtgcgtg taccaaaaga aaatattttc
    13801 cctgaaatta aaggattgaa gggtccattg atgtgtttgt ctactgcccg ctatggcatc
    13861 agctggggtg taattggtgc ggctatggat tgctatgata cagcattgcg ctacagcaaa
    13921 caacgcgtgc agttcggcaa acctatcggt ggatttcaat tgacacaaaa gaaattggct
    13981 gaaatggtta ccgagatcac taaagcacaa ttacttgcat ggcgattagg tgtgctcaaa
    14041 aatgaaggaa aagctacacc tgaacaaata tctatggcta agcgcaataa tgtgaaaatg
    14101 gctatcgata tcgcgcgtga agcacgacaa atattaggag gaatgggtat caccggcgaa
    14161 tattcaatta tgcgtcacat gatgaatctt gagtctgtaa ttacatatga aggaacacat
    14221 gatattcacc tgctcattac cggcgcacat gttaccggtc tcgatgcatt caaataaaac
    14281 aataaatatt tcgcacctgc gtatttcatt tagttaaaat cttgcaaagc acacttatca
    14341 ttatgatttg tgcgcttttt tgtcgatatt caccgatatg catccattct tagcgaaatt
    14401 gcttgctgac aaagccaaga aagaaaagaa aagtgaagta ttatccaatt atattcttgc
    14461 acgtgaattt gcgaaattca gattgcgcgt acgtcgaatc gtcgctgcag tagcattgct
    14521 tgctttcggg gtgagtgcgg cagctttcgg gttactcgga tttttattac caaatcattt
    14581 tgtagatggc ggagctgttg gcgtagcgat gttaagtgcc caggtttcag gacttgattt
    14641 ttcagtattg ttggtggtga taagcattcc atttatcata ttaggttatc gaactattgg
    14701 attgcagttt gctatccgtg cctccatcgg aatcgtggca ttggcattag cagttgaatt
    14761 tattgacata cctattatta ccgaagataa actgctcgta gctgttttcg gtggattttt
    14821 tctcggccta ggtacgggaa tgaccatgcg ggcaggaggt gttatcgatg gcactgaaat
    14881 tatggcactt caactcagca agaaattggg cgtaactgtt ggcgatgtaa tcctgattat
    14941 taatattatt attttttcta ttgcagcctg gttactttca ctcgaaatag cattgtattc
    15001 aactcttacc tatctcgcag ccggacgtac ggttgatttt atactcgaag gtatagaaga
    15061 atatacggga gtgacaatta tttcgccaaa atcggaagac atcagattga tgatcacaga
    15121 aaaattacgt cgcggggtta ctatctataa aggaaaacgt ggtttcggat cgcatgggca
    15181 tcgacaagca gaaattgata ttgtatatac tgtgattact cggttggaaa taaacagatt
    15241 aagtgctgaa atcgagcaaa ttgatccgga ggcatttatt gtaatgcaaa gtgtgaagga
    15301 tacccgcggt ggcatgatca agaaacgggc gcacaaacat tagtacctga tcactgattg
    15361 atacttgtca ccaatcatgg taatttcagg cacaaatcac cgtttctccc ggcaatacct
    15421 ttgtcttgta ttaaaagatg atgtatgcga caaattatag taatcgcatt gctactgctt
    15481 acgctgagca gtcgtgcgca ggtcatggtt gatatcccaa caatgatgca agaggagcaa
    15541 ctcgccagag atgtttatca attagcgaat gaaaaatacc aaatgcatgt gttcgaatgc
    15601 aagataaatg ctgaaacaaa ccattggacc atgctgcgga gttattctga aactccaaat
    15661 ctggaaatgc cggcttacgg tagcttgagt gactcaatct tcaaagctgc cttttcagat
    15721 tatcagacta tggttaccac aagtgccaca gaggcattaa aggcaggtgc tatgatagag
    15781 gaaactgata ttttcgttct ggaagtgaat agaattaaag ccgaagacga acagttgaaa
    15841 gctttgctcg acaaccttat cactgcttca gagcagcatc tgagcagttt tcttacagca
    15901 ctgaaggccg atggcgtcac ttatacacca agcctgctat cggaagaacg cattcagcga
    15961 ctgatgaacg agggcaattg taagccggga gctgcctgca aaggcaattc ctcctgttcg
    16021 cactaatcgc aatgtggata attggtggga aaattctttt tattgttgta aattacaatg
    16081 aaatcaggct tttcatacgt tgttaaataa cgtttggatg tgcatttatt tttcctgaaa
    16141 attgttgtgt tacaagcggt agggtgacta cttttggcac cccgctgaca gcaacaaacc
    16201 gacaccaacc actcagcgaa cgtgaactat ggcagaatct gataacagca cctctaattt
    16261 tcagaaacgc aatgagcgcc gtcgcccaca ggccgatctt tcttcggtag tgtatggcaa
    16321 agtgcctccg caagccggcg acctggagga agcggtattg ggagcattca tgatagacag
    16381 agatgctgtt acgaacgcta ttgaaatttt gcgcgcggat agcttttatg atgacagaca
    16441 tcgcctcatt tttactgcta tccgtcgtct gtttgaaaaa acgcaaccca tcgatttgct
    16501 tacggtaaca gaggaactgc gtcgtatggg gcagttggag gaagcaggtg gtgctttcta
    16561 tatcacttcg ctcacaaacc gcgttgcctc ggctgcacat attgaatatc atgcacgtat
    16621 tgttgctcag aaattcattc agcgtgagtt gattcgtatc tcatccgaaa ttgtgcatga
    16681 tgcatatgaa gatactactg atgtcttcga gttattggat aaagcagagc aaaacctgtt
    16741 caacatagca gataaaaact tacgcaccag ctttagcgat atcaataatc tcttgagtat
    16801 gaccatcaaa gagatcgaag aggccaggaa gcatgaagat atgctcaccg ggtgcccttc
    16861 cggatttagt gcgcttgatc gtgttaccgg cggatggcaa aaatcagatc tgatcattgt
    16921 ggcagctcgc ccggcgatgg gtaagactgc atttacttta tcgcttgcca gaaatgcagc
    16981 ggtggaattt aaaaaaggtg ttgcagtttt ctcattggaa atgagttcac agcagatcgt
    17041 gaaacgtttg atctccgctg aaacagaaat tcccgccgag aaaattatca aaggaaatct
    17101 tgccgaacat gaaatggtgc agttggtgaa attgtcaggg cgactcagcg aagcgccgat
    17161 tttcatcgac gatacacctg ccatcaatat attcgagctc cgcgcaaaat gccgtcgttt
    17221 gaaaatgcaa catgacattc agttggttgt catcgattac ttacaactca tgacggcagg
    17281 agattcttca aaaggtggta accgtgaaca ggaaatcagt cagatatcac gatcactgaa
    17341 atcgattgcc aaagaattga atattcccat catcgccctt tcacagttaa gtcgtaaagt
    17401 tgaagaccgt ggcggcacta agcgccctat gttgtccgac ttgcgcgaat ccggtgccat
    17461 tgagcaggat gcggatttgg tatgtttctt gtatcgtcct gaatattacg gacttgatgt
    17521 ggatgaaaat ggcaatccta ccaaaggtat tgcagaggtg attattgcta agcacagaaa
    17581 tggtgcactt gaaaatgtac gtgtccgctt cgttgataaa tacatcaaat tcgttgatct
    17641 cgatgaaatg tatacaccgc aaaatctaac accgttagca aatattcttc ctgaaaataa
    17701 agctacgatc acacgcaaat caaaaatgga tgatttgaat gatcttgata tgcaggaaga
    17761 accgccattc tgataatagt ggtttaataa acgcttctat ttcgaagctc gtttgaccag
    17821 gaaaactgca atcagtgcaa aaacaatatt ggggatattg gtgccaataa ttgggtgaag
    17881 actgctattg atggagaatg aagaagtaaa ttgaaggaag aaaatataac ccgcactcaa
    17941 agctacgcct gcgataaaat taaaccagtt tccacccctt atttttcgcg atccgagagc
    18001 tgcacccatg atgactaaga tgagcgcaga aattgcggat gatgctcttc gatattgctc
    18061 tacacggaaa tattccaatt cgcccgtgcc ttcactttct ttttctttaa tgtaatcctc
    18121 catttcctga taggtgagaa catccttcac acgaatatca gcatcaaaat cgccaggtac
    18181 aagattgaga attgtgtcca tttgacgtcc ctctttgtat gattcgcttt ggccattgat
    18241 ataccaggct ttataatcga caaactgcca tgtattgttt tctttgtata caattttgct
    18301 cgcgttgatc ttacgtgaaa gtgtcttttt tgctccagtt cctgagaata cttctaatga
    18361 acagttatat ccttcattgc tcttgaattt aaatcgctct agtgaaatga tactgttttt
    18421 atccagcttc agatgtaaat tattgccgta gtttttaggc atatgaatat acttatcttc
    18481 aaattccaat ttgcgtttat tggtatttgg aacaagccaa taattggagt aaagaagaaa
    18541 tccaacagca aacaagccgc aaatgaaata aggtcttaaa aatcgcctga aggtcatgcc
    18601 actgttaagc atggcaataa tctcgctgtt ataggcaagt cgcgatgtaa aaaatatcac
    18661 cgctagaaat gtcagcaacg gcgcaagtat tgaagcgata aagggaatga aatcaatata
    18721 atattgcacc acttctgcag taggtgcatt gcgctctcca aatttatccg acttttcaga
    18781 gaggtcgatt acaatggcga tgctgatgat tatgagatgg aaaaaaagcg tcgacacaat
    18841 gaatttcttc atcacgtacc tgtctagcag tgtaaacggg cgccttattt tttttgacaa
    18901 tcgactcaca atcgtgaact tactttttgc accattaaat tcttccatcc ggcgaaggtg
    18961 cctgcttcaa tatgcatcct cgcttcgcgc accaaccaca aataaaaggc cagattgtga
    19021 atacttgcaa tttgtggccc taatctttct cctgaagcaa acaaatgtcg caaaaaagct
    19081 ttgctgtggc gccgactcac agaagaagtg ctattgatgt caattggact aaaatcctgt
    19141 tcccactttt tattccgaat attgatgatg ccatcagcgg tgaataacat accattcctg
    19201 ccattgcgcg ttggcatcac acaatcaaac atatcaattc ccaaagcaat gcattcaaga
    19261 atatttgcag gcgtgcccac acccattaaa taacgcggtt tatcttgcgg aagtatattg
    19321 caaactaaat ccgtcatggc atacatatct tcatgcggtt cgccaacact taatccgccg
    19381 atggcatttc cttcggcacc ttttgaagca atatattctg cagatatttt tcttaaatcc
    19441 ggatatacgc tgccctgaac tataggaaag aaaaattgct tatgttgata ttttggtgct
    19501 gtagcttcaa agtgcgctat acccctgtca agccaacgat gtgtcaatcc catacttttt
    19561 tgtgcataag atctttcgca ggggtaggga gtgcactcat ccagagccat aataatgtca
    19621 gcaccaatac tacgttgtat atctacaaca ttttccggcg agaaaaaatg tttagagcca
    19681 tcaatatgcg attgaaatgt tacgccttct tctttaatct ttctgatctg tgccaatgaa
    19741 tgcacctgaa agccaccgct atcggtcaat attggtttct tccaggaatt aaatgcatgc
    19801 aatcctccgg ctttttcaat tgtgtcgatt cccggcctta gatataaatg ataagtatta
    19861 cccagaatga tggcagcctg aatatcatcc tccaattcct ggaaatgcac accttttacc
    19921 gtgccaatcg taccaaccgg catgaagata ggtgtgggga tgcttccatg atcggtttgt
    19981 aactcacctg cgcgggcaga tgaatgcagg tctttggctg tcaatttgaa aaacacaggg
    20041 caaaaatagg taattgtgga aagcttgtgc atatgtgaag tgctaataac atcacaaacg
    20101 ccttgcaatg ccttgaaact ctaactttgc ggcagcacaa ttatgggcga ctcaattatc
    20161 ctcgacagta tattttactt gttttgtggt gctgcggcgg tgcagttatt ctacatattg
    20221 tttttttatt tgcgtttggc aagatatcag cagaaatctg tcatgcagga tcatgcattg
    20281 ccacccgtaa gcatcattat ttgcgcaagg aatgaagatt ctaatctaaa gaaaaatctt
    20341 ccacgcattt tatcacagga ttatcctgaa tttgaggtaa ttgttgtgga tgataattca
    20401 gaggatagta ctacagaata cttatttttt ctttctcaaa aagaaccaag actgaagcgc
    20461 accagtacac gcaacagtaa tagaatgatg gcaggaaaaa aattcccgct cacattggga
    20521 ttgaaagctg cgtcgcatga aatagttttg ttgacagatg ccgattgcta tcctgcatca
    20581 gataaatggc tgcgcgaaat gatgaatggt tatggttcgg gagcagagat cgtgctgggt
    20641 tatggcgcat atgaaaaaca aaccggcagg cttaaccgac gtattcgcta tgaaacggtt
    20701 tcttcagcaa tcaattacct gagcttcgca ttagcaggtt taccgtacat gggtgttgga
    20761 agaaatctat cttataaaaa acacctcttc tttaattcga atggattttt cgatcatcgt
    20821 catttgccta gtggcgatga tgacttgttc ataaatatgg tggcacgtaa gaaaaatact
    20881 gctatcatta ttaataatga tgcttttacc tattcagaac ctaaggagac ttgggatgac
    20941 tggcgagaac aaaaaacaag gcacctttca acagcacggt attataggtt taagcataaa
    21001 ttttggctcg gattgcaacc tataacacat atcttatttt ggctttccgg tattgcttca
    21061 ttgtcactct tatacccatg gtggtatata gttgcgggaa caatcatact ccgctggttg
    21121 ttattgcgtt atactttggg taagtcgatg caaaagctgg atgaaaaaga cctgaaagcc
    21181 tatatcgaat ggtatgatat cttgcagata ttctattatt tccgctttgc taaagctgcc
    21241 cttgtgaaaa caaaatatag atgggattaa acgacaattt atcagaccgc gcaaaggaag
    21301 atctcgcact tgtagaaagt gcacgcagag gtgatcaacg cgcgtacggt gaattaatgt
    21361 cgcgctacaa ggataccatt cattatatgg tactcaaaat ggtgcacaat cgcgacgatg
    21421 ccgaggacct taccattgaa gcttttggca aagccttctt gcacctcgag cgctatacgc
    21481 ctgatttcgc tttcagcacc tggttgttta aaatcgccgt aaataatgct atcgatttca
    21541 ttcgcaaaaa acgattgcat acactcagcc tcgatgatga acaagatgaa ttcacacgat
    21601 cggcatttac taatatcaga accaacaatc ccgatccgga agagaagttt attaaggaac
    21661 aacgcgctaa tatcatgcgc accatcctcg atgaattaaa tccaaaatat aaagcactgg
    21721 ttgaaatgcg ctatttcgac gaactatctt atgaggaaat ttcagaaaca ctaaacatac
    21781 cactcgggac cgtaaaagct cagctcttcc gtgcaagaaa cctgctcgca gaaattttgg
    21841 cggatagaag ggctaaaatg tgatggggag gagagaggag agaggagaag ggtgagaggt
    21901 gagtgggtga gtttacctcg ccgagtaggc gggtgggtga aatgcaacac tcaacgcacc
    21961 aatttcacga ctcaggattc acgattcacg attcacgact cacgattcac cggtaacata
    22021 atctctcaaa tattcaaaag gcttgttcag atcgccattt ttacagatgg tagcttcatc
    22081 aagcatacgg gagttatctt gaaataattc attccaaacc gttgaaatta attgctgtcc
    22141 aaattcttca cttgcacttc gcggtaattc atttggtaaa ttatcaattg ccataatatc
    22201 gatgctttcc ggaatgtagg gggcaatctc catgtcggtt gctacatcat agccaaatac
    22261 aggctcattt atggttgtag acctgatagt agcaggaata ccgcctttca catcgcagct
    22321 gacatcagca atcaccttta ttttccaatc agtggcatcc atttcatttt tgtgaaataa
    22381 tgaaggtgcc gcaggattcc agaaaatgca attgatcaat acgtcacaaa cctttgtgta
    22441 tggggaaaaa gtgcaatgat ataattccgg atggctgtga aattcattgc ggtcatattt
    22501 gccgtcggac ttgcgggaat acagatcatc gctgtcgagt acacaaaaca ctgcttcgga
    22561 aaatgtttcg ttgagcagag cctctttggt aacctctctg acaccaatct ttctcagaaa
    22621 ttcaatcgcg cctttgccaa ctcttcctgt acctgtaacg atcatnnnnn nnnnnnnnnn
    22681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnncgttt taagtgttgt tcacctaatc gttgcgcata
    22801 ggtccaaatg gcattatgtg caccgataat tccggcccac ctgccaaaag cgatcacacg
    22861 atttccttca gtatttttaa ggcactcgta atcgatcatg cgcacgtttt tttgcatcag
    22921 cacttgtaat aactttttat tgtgaggctg ttttttgatg gtgtgactga agaaaaaata
    22981 tgtttttccc ggaattaaaa ggtcataagg cacctccttc acaccaaaga gaatatcaca
    23041 gtcgtgcaaa tcttcctgta atgtcacatc cagatctgtg tattcataat cagcatagca
    23101 ccggcctttg ctgggctgaa ctacaatttc aatattggga aactgttggc gaagttgttg
    23161 tacctgagca gggataagcg gtacacgcgc atcagggggg aatttagcct cacggataat
    23221 gcctactttg aactgttgca taagccgcaa agatagctta ccaaatcgcc aaagccgatg
    23281 attttaattt ttcaggaagg tagattgcac ccgtttaatt ccattaaacg acacaaaaac
    23341 atataatccg ggtgccaggt ctgctatggc aattttctct gaaatgcctg ccggtattga
    23401 cttttcggtc attttatata cagttttgcc gtctgcacca tagatgatat atccttcagc
    23461 gttaaaattt gacgttgcag tgattacatc acccgccgga ttcgggtaga gttgcaacaa
    23521 caagtctgtt tctgctattt gttgaatcag tgtatccgta gtatcctcag gttcctcagg
    23581 aatgatatag ctgaacgaga attcggcaaa cattggaata tggtcactcg tgaaatacaa
    23641 agcatcagcc atttcttcag aaactgaggt attcacagga tcgttgatct cattatcgaa
    23701 cctgtttccg tcgttgccaa caggatacat acttcccggg atatagccca ttccggctgt
    23761 atctactaac gacggcgaca tcaatatcat gtcaaaacga tcgtccatac caccggtcga
    23821 tccaccccca aaagcagcaa ttcttgtgct ttgtgtaagg taatcggtgt tggcaatatt
    23881 attccaggtg ctcgtcaaac tatcctgcca gatatctatc acatagcccg gatcggtagg
    23941 atccagcagg tgcaggtaag cttcctcata actgccataa atattaaaat cgccgcaaac
    24001 caagatatgt gccgtgtctg gaagagccaa agttgccttg cgaaggctat ctacctcgcg
    24061 ctgccgttgt gccgcatttg tgccgccgct gctcgccttc agatgaagag agtaaacata
    24121 aaagtcgatg ccgcttgcaa tatgccgcat ttcgaattga ttgatatcgc gcaattctgt
    24181 agtcacaacg gtattcgata aaaaggcata tttatccttg cggtaataca aaccattgtc
    24241 ggtatcataa ctattgatga attccgccat ggcatagttg gtatccacct gcaacatgac
    24301 cgaatctctg aatcgtgcag ccccgctgtc gtctatcagt tcctgcacaa ccataatatc
    24361 aggatggatt tcggatacaa tagtgcgcag ataattctcc ttgaaattgc ctgtataatc
    24421 gaaattcaac acattatacg tcataaacct acaggtatca tgtgcctgca tttgcgcttc
    24481 tgcctgaaaa acggaggcaa tagccaccaa taccaacaaa tatgccttct tcacgtggac
    24541 aaagatgcga aatcggggcg aaatgcctcc ttccattttc gtaattttgc agcgctatgg
    24601 agatttccaa aacgtatgat cccgcgtcga tcgagaagaa atggtatgca cactggttag
    24661 caaacaggta ttttcattca aaacccgata accgtgagcc ttttactatc gtcattccac
    24721 cgccgaatgt aacaggcgtg ctgcatatgg gtcacgtgct gaataacacg attcaggatg
    24781 tcctcatccg ccgtgctcgt atgcaaggaa aaaatgcctg ctgggtgcca ggaaccgatc
    24841 atgcttccat tgctactgag gcaaaggtgg ttaagatgct gcgtgaacag ggaattaaaa
    24901 agtcgtcatt atctcgtgaa gattttttga agcatgcatg ggaatggaaa gagaaatatg
    24961 gaggcatcat ccttcaacaa ttaaaagaac tgggttgcag ctgcgattgg gatcgtaccc
    25021 gattcacaat ggatgatctt tattatgacg atgtgatcga tacattcatc gatctttata
    25081 ataaaggata tatctatcgc ggcctcagaa tggttaactg ggatccggaa gcacgcaccg
    25141 ctttgagtaa tgaagaggta atctatcagg atgttacttc gcgtttgcat tatgtgaaat
    25201 atcaaatcaa gggcgataat gtttatctga ctgttgcaac aacacgccct gaaaccattt
    25261 taggagatac tgccatttgt gtgcatcctg atgatgaacg atataaagaa tttattggca
    25321 aaactgtatt agtgccgctc atcaaaagag aaattccggt tatcgcagat acctatgttg
    25381 atatggaatt tggaacgggt gcactaaaaa ttacacctgc tcacgatcct aacgattatg
    25441 aaataggctt gcgttataat ctgccggtta tcgacatcat gaatgaagat ggcacgctca
    25501 gtgaaaaagc agaaatactg atcggtgaag atcgcatggt agcgcgcaag aaaatggtag
    25561 cgatgcttga agaggaaggc tatcttgtga aaattgaaga gctgaaaaat agtgtaggtt
    25621 tctcagagcg cacaaatgcc atgatcgaac ctagattaag tatgcagtgg ttcatcagca
    25681 tgaaagagat cagcaaacct gcccttgaac atgtgatgaa tggcgatatt aatttacatc
    25741 ccgagaaatt catcaacacc tatcgacact ggatggaaaa tgtgaaagat tggtgtatca
    25801 gtcgtcaatt gtggtgggga cagcgcattc ctgcatggta taatgcaagt ggtgaatttg
    25861 tagttgcgaa aacagaagca gaagcaaggg ctatatttac ttcgaagggt atgtcgctcg
    25921 atggtttccg tcaggatgaa gatgtattgg atacctgggc atcaagctgg ttgtggccga
    25981 tttcggtatt caatggcatg aaggatccga agagtgctga tattgaatat tattatccga
    26041 cacaagtgct tgttactgca ccggagattt tattcttttg ggtggcgcgt atgattattg
    26101 ccggttatga atatcgcaac gagaaacctt tcagcgatgt atatctgaca ggtattgttc
    26161 gtgataaatt gcgccgtaaa atgagcaaga gccttggcaa ttcgcctgat ccgctcgacc
    26221 tcattgctca atatggtgca gatgctatcc gcatgggaat tttattgtgc tctcctgccg
    26281 gaaatgacat catctatgat gataaattaa tagagcaagg tcgaaatttc aataataaat
    26341 tgtggaacgt actgcgactc atcaaaggat gggaagtgac cgaaacgtcg caaacgaaaa
    26401 atgaagccgc tattcaatgg tttgattcaa agctaaatga tactataaac accatcaacg
    26461 atcattttga acagtttcgg atcagcgatg ccttgatgtg tttatacaat ttcatttggg
    26521 acgatttctg ttcatggtac ctcgaattta ttaaaccgga atttggtcag cctattgata
    26581 gtattaccta tgaaaaaaca atagatttct ttgaaggttg cctgaagttg ttgcatcctt
    26641 acatgccctt tatcactgaa gaaatttggc atttgctgcg tgatagaaaa gccggagatg
    26701 atattgtaat cacacaattg aatgacgcgg gtaatatcaa taacaatatt cttgcatcag
    26761 gcaacgaagc gaaggaatta atcagcagga tacgagatat ccgcgctaag aataatgtga
    26821 gtccaaaggt tgctgcaagt ttgtatgctg aaggcgatat caccagcatg caagccttcc
    26881 gcgctgtcat catgaaaatg gcaaacctcg aagtgtttga atctgtgacc ggcgatgtaa
    26941 aaaatgcagt cactttcctt gtgggaacgc aaaagttttt tgttgttgtg cagatggaaa
    27001 ttaatatcga agaggagcgc aaaaaactgg aagaacaaat tgcctatcaa aagggattcc
    27061 tcctttccgt tgacaaaaaa ctcagcaatg agaaatttgt tggcagcgca cctgctgctg
    27121 ttgtagattt ggagcgtcgc aaaaaagctg atgctgaaga aaaaatccgc atactggaag
    27181 aaagcttacg taacctcgga aattgatttt accaataaca atatctttaa tttaaacctc
    27241 aaccataaca aaatgaaaaa aggattcctc gtcattctgg caatcgcctc aatggcaact
    27301 ttaaattctt gcggcaaaaa gaaaggccca agtgcagaaa caaaacaggc aatggcaaca
    27361 ttcgaatctg aatggaaagc cctcggcgat caaatcgctg cttgggaaac tactatgaac
    27421 acagcaatgg atggcatgac acaaatgatg actgccacca gaagcatgga cattactaaa
    27481 ttgtccggtg aatcaaaatc catggcagaa atgaacctga tgatgtgcag caaaattgat
    27541 gagcaaatgg tcgctatgaa aaccggattt gcaaaagcaa aaaccgattt cgctgcagca
    27601 actgacgagt atggtgcatg gaagaaaaag ggacaggaag agcaccttga tgatgcaact
    27661 attacctcag aactcgcaaa ctggacgaca aaactaactg agtggaaaac tacagttgaa
    27721 ggcttgaatc aacaactcag cggaatgcaa gatgcttgta agcaaagttg tgatgccatc
    27781 actgccatgg tgccggcaaa gtaattttta attgatttta ttgaaaggct gccttttttg
    27841 gcggcctttt ttgtgccaag aatgttccaa aacctgttgt tagtagcttc agaggcccta
    27901 gggtattcat tgtaagcatt tgcgaaaatc aataatcact ttgtcggaat gtgcatattt
    27961 tgcctgttgg ctcaaaggca tagggttata tttgagtgtt aaacccatat tcatgcgcaa
    28021 ttttctgctt atttgcttgc ttttatcaac gataagtcta accgcacaat cgccgggtca
    28081 aaccgtccga ggcactatta ccgacgcagc atcgaagtat ccgcttatcg gcgctactgt
    28141 ccttttgtat gcagactcaa ccatgcttgg tggtgctacc accgatgaac aaggtgatta
    28201 tcgcattacc aatgctccgg tagggcgtgt taccctgcga tttacatatg ttggttatga
    28261 ggacatcacc atgagcaatg taatagttac cggcgggaag gaagtgatta tcaatgttga
    28321 attggaagaa ggtgccgaag cacttgatgc agttgttgtt acggccaaat cggacaaggg
    28381 tgaggtaaat aatgaaatgg caatcaatag cgtgcgcacc ttcagtgttg aagaaacaga
    28441 tcgctatgca ggcagtcgcg gagaccctgc tcgcatggca tcaaattttg ccggtgtgca
    28501 aggtgctgat gattcacgta atgatattgt tgtgcgcggt aattcaccaa tcggcgtgtt
    28561 gtggcgattg gagggtatcg acattccaaa tccaagtcat tttgcaattg ccggtactac
    28621 cggcggtccg gtatcaatta tcaataataa atacctcagt aactccgact tttttacagg
    28681 tgcttttcct gcagaatatg gcaatgctat cgccggtgtt ttcgatttga atatgcgaag
    28741 aggcaatgat gaacaacatg agttcagtgg acagcttgga tttcttggaa cagaattgtt
    28801 tgccgaaggg cctatcaata aatcgaaagg ttcatcttat ttggcgagct atcgatattc
    28861 aactttacaa ttatttcaat tcatgcacat tccgatagga actaccgctg tgccaaatta
    28921 tatggacgga gcatttaaac tttcttttcc aaaaggtaat gccatgtgga atgtatttgc
    28981 catcggcgga aaaagtaaga tagatatttt ggtaagtgaa tacactaagg aggaaactga
    29041 attatacgga gatagcgaca gagatcaata ctttggtaca gctatgtcgg ttgcaggtgt
    29101 ttcatatgcc aggcaataca atccatcagc atatggtaaa tgggttgctg catatagtgt
    29161 acaggaaaat catgctaatc atgctttcgt attcagagat gtgaatttcg atatagattc
    29221 catagtgccg aatatgaatt attctttcct cgatcaaaaa gcatcgctga tatattcatt
    29281 gaataaaaaa gtcaacagtc gccatagttt taaaacagga gcaagctttg aatcaatatg
    29341 gtataatcat atcgattcta atagaaatgt gtatacctgg acttgggata accgctatga
    29401 ttttgaaggg aataattatt tggcacaagc ttactttcaa tggaaatata aatacaatga
    29461 acgcctgttg tttaccggtg gcttgcatgc ccagatattt accatcaaca gcaattcaaa
    29521 atccctggaa cccagagccg gtatgcaatg gcgtgtggat gacaggtctt cgttaagcct
    29581 gggtttggga ttgcacagcc agatgttgcc aagctatcag tatacctata tgcattatca
    29641 ggcagatgga agtgcattgc tcatcaatga agatgtcgga tttatccgta gtgcacatat
    29701 gatactcggt tatgatagaa agctctccga taatctgcga gttaaatcgg aagtatatta
    29761 tcaatatctc tatgatgtgc cggttgaagt tgcaccaagc gcattttcat tggcaaacga
    29821 agggagtggg ttctcaagat ttttccctga ctcattacag aatacaggaa caggtaaaaa
    29881 ctatggtgcc gaacttacca tcgagaaatt tttcagcaat catttctttt tcctgataac
    29941 cggatcatta tacgattccc gatatgttgg gtcagatggc attgaacgca aaaccgattt
    30001 taatggcacc tatgccggta atatgctcgg cggttatgaa ttcactatca gcgaccgcaa
    30061 tgtgcttacc gttggcatta aaacaaccta tgcgggaggt aaactcacca cgccagtcga
    30121 cacagcagct actattgcag caaaggaact gatctatatt gattctctga caaatacgat
    30181 acaactcaaa gattattttc gttttgatgt gaagcttgct tatcgaataa attccggtaa
    30241 gcgtagtgtc acgcacgaaa tcgctctgga tcttgtgaat gtattgaata ttcaaaacgt
    30301 gttgggatta acttatgcgc caaaccctgc agatccaacg gccagtccgg tgaaagaaga
    30361 gtatcagtta ggctttttac ctttgttcta ttacaaaatt gatttttgag gcatgacata
    30421 ctcaaccatc ctttatacta tagccgataa agtaggcacc atcaccctta atcgtccgga
    30481 taagcgaaat gcattcaatg cggatgtggt agctgaatta actgaggcat ttaccgatgc
    30541 tcaggaaaat gaatctgtga aagtgattgt gctcgctgct tcaggggagg tatttagtgc
    30601 aggtgcggat ctgggatatc tgcaatcgct gcaacagaat tcttttgaag aaaacgtagc
    30661 cgattcgcaa aaattaatgg ccttgtataa attgatgtat accttgtcga aacccatcat
    30721 cgcaaaaatt gaaggtcatg ccattgcagg tggttgcgga ttggcgagtg tatgtgattt
    30781 cagttttgct gttccggaag cacagtttgg atatacagaa gtgaagattg gatttattcc
    30841 tgctattgtg atggtatttc tgctgcgtaa aattaatgag cgacatgcaa aagaattatt
    30901 gctcaccggc aaattaataa ctgctgaaac agctgaaaaa tatggtctca tcaatgcagt
    30961 gattcccaaa aataaaattg acgaagctgt atccggttta tgtgttgagt tgatagctca
    31021 gacgagtagg gtagcagtgg ccactacaaa atcgatgata gcggcggtgc aggaaatggg
    31081 attggatgaa gcattgcaat atgcggcaga aatgaatgca aaggccaggg caactgatga
    31141 ttgtcaatat ggtattcgct ccttcctgga gaagaaaaaa cctgaatgga aataattatt
    31201 gtaatgtctt tccttcggct aacaatgcca atgcatctaa aatggcatta tacacttcat
    31261 ccaatgcttc gttggtgata caatatggcg gcatgatata gatggtgttt cctaatggtc
    31321 gcagaagcac acctcgtttc atgaaaaaat gatagagcat gtcgcgtatg ctgttaaaat
    31381 atccggtatc gttggaagta acaaaatcca ttgctaacac tgttccgcat tgtctgatat
    31441 tttttaccac aggtatatca ctgtgactgt acacttgtaa tgcggctgta aaatgttgat
    31501 gacgtgaagc tatggttgtg agtgcatgat caaaaccggg ctgcaaacat aaatcgacac
    31561 ttgcatttgc ggcagcacaa gccaaaggat ttgccgtata gctatgcccg tgataaaggg
    31621 ccttcatttt atcttcacta tagaatgcca tgaaaattgc ttgcgtgcat atagtaacgc
    31681 cgagtgccat tgatccaccg gtgatacctt tacttaagca tataatatcc ggctgatgat
    31741 tggcataatg tcccgcaagg aattttccgg ttcggtaaaa tcctgtcatt acttcatcgg
    31801 caatacatag tactccatgt tgtttggcgg tgtatatcat tgtatctaaa accataccgg
    31861 tatacatgag cattcctccg gctcccatga taagtggttc gtaaataaat gcggccacat
    31921 ctccttccga caacgctgct tctaattctt gcatacaaat gtcttcattt cccggaaggg
    31981 gaggggtgat ataggtgacg tcaaataatt tatcgtgaaa agcggcagta aaagcaccac
    32041 gcgcgctgac actcatactg ccaaatgtat cgccatggta gctgccctcc aatgcaataa
    32101 tgcgatgttt ttttgctcca agattttggt gatattgcaa agccatttta agtgccactt
    32161 caacggcggt acttccgtta tcggagaaaa acattttagt ataatcgctg ccgcacattg
    32221 ctgttacacg agaagcaaga tgcattgccg gagcgtgtgt aaaccctgca aaaattacgt
    32281 gttccagttg tccgaactgc tcatgaactt tttgtcggat atagggatgt gcatgaccgt
    32341 gaatattcgt ccaccaactg gaaacggcat caataatagt attgccatca gcagtaaata
    32401 acagtgcacc tttccctgaa acgatttgta caggttcggg ggctaattgc atttgggtga
    32461 atggatacca tataggacca tcatcacgca tatcaggata tttttaattg ctgcatgcgc
    32521 ttgcgaatga attcggcatg cttttttata aatggtttgt ccagaacgtc agtatgtgct
    32581 acgcggccaa taaccgggat gtcggaatat tgagtgatga attgttcgct ggaaacattt
    32641 tccataccat tgaaaataat tcctgcaacc ggtatttgtt ttgctcgcaa cacagcgcag
    32701 gttaacagtg tgtggttgat gcttccgaga tagttctttg aaacgacaat cacagacgct
    32761 tcccattttg cgatcatatc caacatcaag gtggagttgt tgatcggcac cattaatccg
    32821 ccggcgcctt ctatcactaa tgtgtttgat gtttcaggaa cgacaatatt atctaaatct
    32881 atttccacac catctaaagc agcggcagca tgtggcgaca tgggttgctt caataagaat
    32941 gcctctttat gaaattgact ttttggattg gaaataagat tctgcacgcg ttgtgtatcc
    33001 gtataatcca aaccaccact ttgcaccggt ttccaatagt cggcaaataa tgcctccact
    33061 accactgccg aaacaacggt cttccctata tcggtcccta ttcctgttat aaatattttt
    33121 ttcatttggt tggggtgatt agcatgtggt atccggtatt gaaaatatta ggatgatttt
    33181 aattgctaaa ttaatgtgca acttccaatt atcatggata tttaactatg gtgttcaagt
    33241 ccagctttgg cggaatgtcg ctgcgctcca tgagcaaaat cgcaaccgat ccactcaact
    33301 tcaaagtaca ttcttccaag cctcaaaaaa tcctctctcc cgtttcctct ctcctttctc
    33361 ctctctcctt tctcctctct cctttctcct ctctccttca atagaacatc attttcaaac
    33421 agaacactga tcctccgctt ttcataaatt cggtggtatc ggtttcgatt acggggtttg
    33481 cactttgttg gatgatgttc aaagaaacgg gattgcctcg ttgaattaca gctgccgatg
    33541 aatttttatt ggtgccataa ataatatgtg cattgcaagc aaatccgtcg atggcttcat
    33601 cttccggtat ttcatacaca ccttcaaaca tgcggcgaat aatggccagt gattccggtg
    33661 tgaatgcatc aggggcaata agtacttcgg tgtgatttaa tgggagaaag caagtatcta
    33721 aatggtagtg atgctcattg ataagttcga gagtgataat aggcgcatca acaatgcggg
    33781 aaatttcatc gtaaactgct ttttcactgc gaaacccata tgctccatat aaaacacgtt
    33841 ttccgggatg agcaatcaaa tcgcccatgc cttcaaaatg atatttgctt tgcagctcca
    33901 caattttata tccgtggtcc tgataaaaat cttcgatata tggaatttca ttttgtcggt
    33961 tttcatggcg catgcggctc atgatggcaa ttttctcgcc attccacatc acccaaggaa
    34021 aactctggtt tgcacaaaaa accatatcct ccatgccttc cacaccattg atgacaacaa
    34081 catcatcaag gaatttctgg tttttcaatg cgagataaga attgcggata ttatcccatt
    34141 gaatatttgc acgctccttg ttgatattgc ctttttgatg ttgcatgtat acatttttca
    34201 catcaacaac attatagtag tcgggcgtgc acatcattac cttgcgtggt tcattgcgat
    34261 tgtcgcaatc ttcaatctgg aaatgtactt ctgaagggga atatgctttg gcaaaaaccg
    34321 gtgtcatgta tgtattattg atagctatta atgaatgggc atcaagattt gtgtagatgc
    34381 ttaaattgtt ttttttaaat tacttattaa atcgtggtga cacaacgagg tctttatttc
    34441 ctgccgtata agcataaaat ccttcacctg atttagcacc aaggtaacct gcagtaacca
    34501 tatttaccag tagcggacat ggtgcatatt tcggattgcc aaaaccgtcg tgtaacacat
    34561 tgaggatgct aaggcaaacg tcaagtccga taaaatcagc cagttgcagc ggtcccatag
    34621 gatgagccat gccgagtttc ataacggtat cgatttcata tacgcccgcc acgccttcat
    34681 ataaagtata aatggcttcg tttatcattg gcattaatat gcggttggca acaaatccgg
    34741 gataatcatt tacttctaca ggcactttat ccaatgctgc agataattcc attatagttt
    34801 tggctgtttc atcggaggtt gcatagccgc ggattacctc caccagtttc atcaccggaa
    34861 ccggattcat aaagtgcatg ccgataactc gatcagcacg ttttgttgct gctgcaattt
    34921 tagtgatgga aattgatgag gtattgctgg caagtatggt atgtgctgca catatttgat
    34981 ccagttcagt aaatattttt aatttaagat caacgcgttc tgtagcagcc tcaacaacca
    35041 ggtcggcatt agtgctgtga tcaaaatttg cataagtggt aatattggaa agtgttgctg
    35101 ctttttcatc tgcagtgatt ttttccttgg ccaccatgcg atcgaggtta ttggtgatgg
    35161 tagcaaccgc tttttgcaag gcaggttcag aaacatcaac aagtgtgacc ttgtggcctt
    35221 tcatggcaaa tacgtgtgcg atgccgtttc ccattgtgcc ggcaccaatc acagttacat
    35281 ttttcatagc gcaaaagaat ttgcgcaata ttagtgcata atcaacacct tttcggccgc
    35341 aatccgctca gaacgggcgt tttcaacaca aagttgataa gttccggcgg ggaggttttg
    35401 tgtggggatg ctcatggaaa gattatctat ccttcctgtc catattttac gcatcgaagt
    35461 gtcgtaaagc gataactgca tacccatcaa ttgctgatca acagttgcaa caattagttg
    35521 attggcgggt ataggatata ctgtaagcga gttatcgctt aaattctcaa ttgccccatt
    35581 aaatgaacaa tctgtttccg gaatgtataa tggttgatcc ggtatggatg catcatatat
    35641 tccaatgcaa aaatctcctg atgttacggc agataattcg aaagcaccgc gcttgtcatt
    35701 ggcacttccc caagtattta atgtgtaatc tggatagatt atccagtcac cttgcgggcc
    35761 gtttctgtat agtagtacaa ggctatcttc agaatttgta atgaaatcat tatctaaata
    35821 accgctgcct atggtagctt gtccgttgta tagaaattta gccgtggcat caccggtaac
    35881 gggaatatta gttgttattc tccaaaagcg atcagtgctt acatgtaatc ccggatgtgc
    35941 ggcaatataa ttattcggtg cagcccaaaa atgttgcgca taaatgaaac ccggttctgt
    36001 tacggatgta atttcaatat ccatcatggc attatcaagc gaatacgtgc cgggttcggt
    36061 aaaatgcact tcatcggatg ttaccgcatc atttagttta ttcctgtaat caagcaccgt
    36121 gtatccccag gatcccggat tcggtattcc aactataatt aattgcctgt cacccgacat
    36181 cactacatca acagagtcaa acagattgaa atcataatcg aaaaagtata cagtgagtgg
    36241 cacattatta ccataatgtg ttgcatgaga caaatactgc tgaatatgta aac
//
LOCUS       DAOQOU010000010        35688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_490_length_35688_cov_6.949710, whole genome shotgun sequence.
ACCESSION   DAOQOU010000010 DAOQOU010000000
VERSION     DAOQOU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 35688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_490_length_35688_cov_6.949710"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..530)
                     /locus_tag="PLM90_01675"
     CDS             complement(261..530)
                     /locus_tag="PLM90_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88103.1"
                     /translation="MFTDRNIDIFLAGLVTRVALSFPIAYIRSIALVSNNKKIADCNW
                     ESDPRGTVTRYDDLHVPVAAEQVVVEGLDTNGQPLMITAAIASLH"
     gene            complement(761..1870)
                     /locus_tag="PLM90_01680"
     CDS             complement(761..1870)
                     /locus_tag="PLM90_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMZ88104.1"
                     /translation="MFRKHRIWILPIAFFATIMLFTKCDPDDPITPDDEWVYDDTPYT
                     ADHVNGIPPMPVFTTNPLTVKGVELGRMLFYDPILSADSTLSCSGCHSVAHAFSDTTS
                     FSIGIDGLPGKRNAMPIFNLGYAVAINATFFWDGRAATIEDQTLMPIQDPLEMHEILP
                     NVITKITRSEFYPKAFYEAFGEEVITTELVARALGQFLKSVTSGNSRYDQAMAGEIFL
                     TDEETLGFTLFNALDGADCFHCHGVNGGLFTDYVYRNNGLDDATHYTDFADAGLGAIS
                     GDTADYGKFKTPSLRNIALTAPYMHDGRFATLEEVLDFYSEGVHDTPFTDNLIQFAYQ
                     GGVQLTAEEKAAIIAFLKTLTDTNFETNEAYQNPF"
     gene            complement(1901..2911)
                     /locus_tag="PLM90_01685"
     CDS             complement(1901..2911)
                     /locus_tag="PLM90_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMZ88105.1"
                     /translation="MRKLISILFLSAILVSCSEVIPEEKAYELQIPPGFDVPQIPEDN
                     ILTDAKIALGKKLFYSTALSVDSTISCASCHRPENAFADHLAISHGVMGRTGFRNAPS
                     LQNVAWYTVITMDGGNPSLETQPYVPIETHEEMGFNMVLLCERLNTDAEMVTSFNDVF
                     GSNPDPYGVTRALAAFERTMISGNSRYDQYAYQGNIHALTAEELQGKDLFFSDALGCA
                     NCHSGFLFTNNTFENNGFFSDYSADSGRARITWLHEDVGKFKVPSLRNVGVTAPYMHN
                     GQVETLDEIIDMYAAGGSGHENQSVFITGFSITPAEKEALKAFLLSLTDISFMQNPDL
                     LE"
     gene            complement(2926..3729)
                     /locus_tag="PLM90_01690"
     CDS             complement(2926..3729)
                     /locus_tag="PLM90_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88106.1"
                     /translation="MKRNFGSALLAICTLLTVSSCSKKEGCTDPTATNYDPDAEKNVG
                     CLYEETYAVEMHVHQFLNDIELEEEMPYVINGVNTNLNLAQFYVSGITLVDANGNEFK
                     QDGLYLLVQPETEEYAIGDFPAGDYTSVKFDIGIDSATNHGDPSLYPIGDPLGAQTPS
                     MHWGWSFGYIFMRIDGEVDSDGDGTPDPAGAFEMHLGTDHYLASIEVEMPISIGQGLE
                     NIVHLQADYTSFFTGVDMVNDNTTHTTDNVTLANLLLDNIYTMFSPGEE"
     gene            3864..4259
                     /locus_tag="PLM90_01695"
     CDS             3864..4259
                     /locus_tag="PLM90_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88107.1"
                     /translation="MKRLRAIFGMMMLVLLFGITSGFNLFTHLCLMTGESGVAPVSMS
                     MDCCGNQLPDAPVTLSMNCCIEKTQFIKFEYSGSVQNQVGYTDYADLDYPVIQIQDEI
                     QADVVNLNSFNLPPPFPGDIRVMQCIYRI"
     gene            4393..6573
                     /locus_tag="PLM90_01700"
     CDS             4393..6573
                     /locus_tag="PLM90_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88108.1"
                     /translation="MAAPVFAQQMVEGEVMTNDDKGKMIPLPGANVYWEGEYSGVISD
                     DEGHFSIEQKTTSQRYLVVSYVGFSADTFDVQTTKHIHVMLSPDTELKTVVVEGAQSS
                     KTINTVDPIGTEQLNKKELLKAACCNLSESFETNNTVDAEFSDAVTGAKTIKLLGLDG
                     VYAQIMTENMDNVRGLSSAYGLTFIPGPWIESIQITKGPGSVVNGYEGITGSINTEYK
                     KPFDADEETARLDVYGNQFGRWEANAVYKHPFNLKWSTGLYGNVSQMQQKVDHNDDGF
                     LDVPMNSTYTFMNKWNYFSGGMHEAQFGVKYINSNVTGGQTSFDVNSPRTTENGYGVG
                     ADITRWELFMKNGFVFKRPSTSIGTILQFADHNQTGFYGLNNYAGAEQFIKAEAIFVT
                     YIGNTNSTIKTGLNYLYDNFDESFDSISYLRNESVPGIFAEYHYQFEKKWSVLAGLRV
                     DQHNLYGTFVTPRMHVKFTPKENTTLRASAGKGYHIANVLIENSSILTSNRTLIIQEP
                     LEPEEGWNYGISLIQQFKLNHREGTFTLDVYRTDFINQVVIDMDANVNEINVYNLKGE
                     AYTNVLQAEINYEPIKRLELRAAYKYVDSKATYDGKLLEVPLTFKDRGLFNVAYNWKK
                     AGLSADATAQFYGKTRLPDLSENHAAHDFGTNSEPYIQLLAQVTKSFGDLDVYVGSEN
                     ITGYTQHNPIIDATDPFGENFDAGVIYAPLMPRMFYGGLRYIFN"
     gene            6601..6939
                     /locus_tag="PLM90_01705"
     CDS             6601..6939
                     /locus_tag="PLM90_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HMZ88109.1"
                     /translation="MKKILAIIGLFMAIGMQTSFAQNSGKQYIEIKTSAVCGTCKAAI
                     ETALEKVDGVKSADLDLETKVVSIKFDGDKTNADALKTAITEAGYTADEVPANKVAYD
                     NLHPCCKSEQ"
     gene            complement(7001..7681)
                     /locus_tag="PLM90_01710"
     CDS             complement(7001..7681)
                     /locus_tag="PLM90_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ88110.1"
                     /translation="MRCLIIEDEPLAQERIKQYIARLPFLEMVGSFDNAIDALLFLRE
                     HHIDLLFLDINLGEMSGIQLLEVIKPNCPVIITTAYQEYALKGFELNVTDYLMKPFTF
                     ERFYQAVEKARAGTQMVVTQEKAFIFVKTEHRLEKVMLDDIQYIEGMRDYRRVHLSDK
                     SIMTLQTFTEFEKILPPQKICRIHKSYMVSISKITSIEKDRVLIAGKRLPISETYKKS
                     FLDIIGAK"
     gene            complement(7684..8748)
                     /locus_tag="PLM90_01715"
     CDS             complement(7684..8748)
                     /locus_tag="PLM90_01715"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ88111.1"
                     /translation="MKKYVVVLLNLGYWLVFMLLILVIFGLISSVSPTSDESDKPVLR
                     NWLGFMTSTTILPGVICFYLNYFFVFNRYLQKRKIPQFFIAVIVTALICGVVGAFSGA
                     APWLIGDEYYIANMSIGNLIAIVVFFGFVAMLNGVIGLVMRGFISWYGDIRIKESLAA
                     KNTEMELSLVKSQINPHFLFNTINNIDVLISKDPEKASEYLNRLSDIMRFMLYESKPD
                     RILLSREIAYITKYIDLHRIRSNNTDYVHFEVIGDPSEYMVPPMLFIPFIENAFKHVG
                     DKKKTPAINIKLIIENNSLLFNCCNHYFPDKIQDADTLNGGLGDKLIRRRLELLFPNA
                     HTLQLTTDNEQYCVSLKIIL"
     gene            complement(8782..9549)
                     /locus_tag="PLM90_01720"
     CDS             complement(8782..9549)
                     /locus_tag="PLM90_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88112.1"
                     /translation="MMILQSIKSEWLKTKHSIATWLCLIGGTFIPLIFLIGMLKDHRT
                     IDMYQGELNAWTYYFFQVWQYMQVFLLPMGLILATSLITQVEYRNNTWKTVHTTPQSY
                     TAIFMAKFIVLLGMTGLFFLYFNATWYLSAAIPSLIFAGHFPPVDFPWNQFLTSNGKI
                     LLTCLPVLALQFLLSLHFKNFLVSIGIGLLLVVGSLILTGQWEYAYTIPYSYPALTIM
                     GDAAQLEKIHIYAWCLGYFIVFLTFGYVLYVTRKAKG"
     gene            complement(9552..10466)
                     /locus_tag="PLM90_01725"
     CDS             complement(9552..10466)
                     /locus_tag="PLM90_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ88113.1"
                     /translation="MSAPENCIVTENLTHRFGSNELVVQDVNLVIPRASIYGFLGPNG
                     AGKTTTLRLILGLLQKQQGKIYIKGMPLHKHRTLLMRSIGNLIESPSIYAHLSATENV
                     RIQQILFGTDRKRIQQVLQLVGLEKTGSKPAGKFSLGMKQRLGIAIALLHSPEILILD
                     EPTNGLDPNGMIEIRELLLRLHKENGMTILVSSHLLAEIEKLATHVGVIHNGILRFQG
                     STTELADLQRHQKGTLIATDNITLSKQIFDEQGISYHIAENVFHVSVENIQRVVELNR
                     TLNERGVGVFKIEAQKHDIETIFMELIK"
     gene            complement(10604..11401)
                     /locus_tag="PLM90_01730"
     CDS             complement(10604..11401)
                     /locus_tag="PLM90_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88114.1"
                     /translation="MKNLKLPIFAISIISIHACTTKPNVATPCTDIFETPKVIQHPTN
                     TPLENLLMDTMSFANYWIVEKYRFNDEIYYRKHPHTKYIKFKSDMVAESMLTQLLDTT
                     NNHGFQIITDYTTTLYDYYGDDLTPYACFPVFIVNPTQSTKVFVAKDSYVFAIQEAMD
                     PHNEQWYPIECLPYDFCGNGYYGIKVHPKEFVMIVAPKFQGDTKGMLRIRLKIGEDIY
                     VSQPYEGYFNSGQFVPPYFLQKRIDDPERLKYGIVRDFIGSVPKVVN"
     gene            11514..>11746
                     /locus_tag="PLM90_01735"
     CDS             11514..>11746
                     /locus_tag="PLM90_01735"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ88115.1"
                     /translation="MAQKITTFLTYNDQAEEAVEFYCSIFPDAKIVSKQYYSDSIPGL
                     GGKLMSATFTLFDQTYYALNAGPHFQFTEAISL"
     assembly_gap    11747..11846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11847..12058
                     /locus_tag="PLM90_01740"
     CDS             <11847..12058
                     /locus_tag="PLM90_01740"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ88116.1"
                     /translation="RYWNALTEGGKEDRCGWLKDKYGVSWQVIPKQLGMLLSHPDKAK
                     ASRAMQSMMGMRKIDIAELERASEG"
     gene            complement(12143..13153)
                     /locus_tag="PLM90_01745"
     CDS             complement(12143..13153)
                     /locus_tag="PLM90_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88117.1"
                     /translation="MKNLQFQILMLLFATTALMQAQPTITSATAGGPGDEFTNKMIDA
                     ILFDPGASGADVTWDFSGVTLTGETNDYAFITAAATGQSAAFPDANVAADNGMGNYSF
                     YKINASVYENHGIYTPDVTVLYSDPEELMTFPITYNATNSDDFYSTIDAGVEIIRSGT
                     VETVADGYGTLKLPDGTYTDVMRVFVHEVYSDVTVGVPVDIDYDMYIWFWLKEGVVGP
                     LFTYYFSSVSTGGFPTISQVAMINESNSTVGIKDQTAQTTLKAFPNPANDMVHVSGVS
                     ASAKKIILTDLLGNNVSEIENPTISEGMISFSSATIPSGMYIAQVVDENSVQSVNIQI
                     LH"
     gene            13485..14354
                     /locus_tag="PLM90_01750"
     CDS             13485..14354
                     /locus_tag="PLM90_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ88118.1"
                     /translation="MRQLKITKSITNRESESLEKYLQEIGKVDLVTAEEEVTLAQRIK
                     QGDQLALEKLVKANLRFVVSVAKQYQHNGLSLNDLINEGNVGLVKAAQKFDETKGFKF
                     ISYAVWWIRQSIMQALAEQSRLVRLPLNKVGSLSKINRAFSELEQKFEREPTPEELAE
                     ILDLAPDEIKNTLSVSSRHVSVDAPFDDGENSSLLDVLENRDVERTDEKLDYSHSLKV
                     ETERTLATLTERERDVIKLFFGIGVPYAMTLEDIGEEFGLTRERVRQIKDNAINKLRS
                     STRNKALKAYLAQ"
     gene            complement(14492..15250)
                     /locus_tag="PLM90_01755"
     CDS             complement(14492..15250)
                     /locus_tag="PLM90_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HMZ88119.1"
                     /translation="MVFGLLLDFELRGAIVGFIIGCFMDTEYVFPEKKEKRDFRVSFM
                     MLGAFVMQTPGVGSKLSSNVVRSALVNTFGETYTQRRLSFFYELLRQRIQVEAICGQI
                     QQYASVGEKNTLLRFLFELGEQPGVNKENLHHSINYLAARIGVPFETVQQVYNTVKGK
                     MRDQQYSEEQTYEEQAHRPEQEFNSLSSSFTTLGLASTANEKEVKRAYYRLAKKFHPD
                     SNPQASADEKKLLESKLRLVIEAYERICEVKNWK"
     gene            complement(15345..15950)
                     /locus_tag="PLM90_01760"
     CDS             complement(15345..15950)
                     /locus_tag="PLM90_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HMZ88120.1"
                     /translation="MNSTVLKKEFFTQTDTVGIARALLGKTLHTKFQGSHTSGIICET
                     EAYCGVEDRGCHAYNGRLTDRTRIMYAEGGVAYVYLCYGIHNLFNVVTHTTGEPHAVL
                     IRAIAPVGGINHMLKRRGLSDSTPQLAAGPGLVAQCLGLTTKHSGLSLFGEHIWITDA
                     PVLPDNDIIAGARVGMNFSGPYKTIPWRFRIKGSPNTSKAR"
     gene            15995..16699
                     /locus_tag="PLM90_01765"
     CDS             15995..16699
                     /locus_tag="PLM90_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ88121.1"
                     /translation="MASLYLIPTILSEDALHTIPQYVHEITLRIRIFIVEDERTARRY
                     LRKTGYSVSFDDVTLLPLNVNTAPEEISGYMQYFSGDTEIGLMSEAGVPAVADPGSII
                     VNKCHTIGTRVVPLVGPSSILLALMASGMNGQRFAFHGYIPLKNPERKNQLQAMDIAA
                     KKGETQIFIETPYRNNQLIQEIISSCHGDTLLCIATDITGTHEKIKTLPVSAWKKNMP
                     QLDKTPTVFLLGTASK"
     gene            16788..17357
                     /locus_tag="PLM90_01770"
     CDS             16788..17357
                     /locus_tag="PLM90_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HMZ88122.1"
                     /translation="MIIIQQTLVSDEILDEKFACNLQACKGACCVEGDEGAFVEPEER
                     KMLEKVYPKVKPYLTKEGIDGIEKQGLWVTNSEGVLKTPLMKDGPCVYTIWEGGIALC
                     GIEKAYLDGKVKWQKPISCHLYPIRLSKVGEYEALNYEEWDICKPACKNGKSLGIPVF
                     RFVKTAIIRRYGQEYYDALEATYAYRKGE"
     gene            17418..19016
                     /gene="nadB"
                     /locus_tag="PLM90_01775"
     CDS             17418..19016
                     /gene="nadB"
                     /locus_tag="PLM90_01775"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HMZ88123.1"
                     /translation="MHKVDFLVIGSGIAGLTFALKTARKFPSRTIAIITKAEEDETNT
                     KYAQGGIAAVWDFEKDSYEQHINDTLIAGDGLCKRDIVEIVVKEGPERVREIINWGAD
                     FDKTKEGEYALGKEGGHSQFRVLHHKDVTGKEIEKTLLEEAQSLPNITFFTHHFVIDI
                     ITQHHLGRIVTRHSPDIQCYGVYVLNKENKSIETVLSRITVMATGGAGQVYKSTTNPL
                     IATGDGMAMVYRAKGRVANMEFVQFHPTSLYQPGVSPSFLITEAIRGAGGVLRNSNGA
                     LFMQKYDARESLAPRDIVARAIDNEMKLRGEDFVYLDCTPIAKDELLNHFPNIYEKCK
                     SIGLDILKDPIPVVPAAHYMCGGIQTDAFGHSSIQYLYACGECSSTGLHGANRLASNS
                     LLEALVFANRIFIDACEKIDGIALADQIPDWNAEGTDDPKEMVMITQSVRELKEIMTN
                     YVGIVRSNERLDRALTRLHLLYEETEKLYETTTLSPQLCELRNLITVGFLITRGAAIR
                     KESRGLHYTTDYPHHSKWAEDSMF"
     gene            19111..20388
                     /locus_tag="PLM90_01780"
     CDS             19111..20388
                     /locus_tag="PLM90_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88124.1"
                     /translation="MMRVFLFALSILCVTHFTAAQDYCSMDRFDTYLWAESDIEVFTN
                     LEYGQAVNNSGNNEHLFLDLYHPPVAEDPLTYKPCVFFVHGGGLVGGDKDSEGAKDLG
                     YLYARMGYVFVSIDYRVGWNNGDADDGCGGDSTDLRRATYRAVQDTRAAYRWIKAYGP
                     LYGVDTNNIIVEGNSAGSMLLLYALYADQEDYDPMFYDELGSIDSAVNELTNFGFDPI
                     GCITEAAGIEDTTLLLIKDVPHLFFHGTCDSIVPFFHGPTFFCFQPFPYPTMNGSWPI
                     VQMFKRNDRNYHFYIAEGAGHDAVIPDSIIVYSAPFIKEILCNASTTKEFYRVLGKFK
                     CAVANEGELVITDVYPNPVVNTIYLEVTSTRQREITIDVYNNLGQSVLSGELPFTPPI
                     GEYTIDLSHLTRGIYYMRLSQRQEEYVLKFVKY"
     gene            complement(20399..21157)
                     /locus_tag="PLM90_01785"
     CDS             complement(20399..21157)
                     /locus_tag="PLM90_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ88125.1"
                     /translation="MHKITALIPTFNESHNIDAVIRSVQWCDEILVVDSFSTDDTVER
                     AKRLGARIIQSEYINSASQKNRNIPNASHQWILLVDADERVTGELRNEITALLKKSEI
                     PCDAYWISRRNFFMGKEIKYSGWQGDKVIRFFNRDTCRYEEKSVHAEVVTSGKIGHLK
                     SKLDHHTYKDVFHYMEKWDRYTTWSAQDAMKKNVNPGFYHFVIKPAFRFIKHYFLQLG
                     ILDGKAGYIISSLSAKAVFMRYVKVEGMRKMQSK"
     gene            complement(21150..22397)
                     /locus_tag="PLM90_01790"
     CDS             complement(21150..22397)
                     /locus_tag="PLM90_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ88126.1"
                     /translation="MRSLLRVFPNQFFASLAILGLIVGLVTSKVVLSVATITLMCNAF
                     INIRAGSNFRKWFSDTTAMLCAALFLVYLFSGFWSSDIGYWVDRCRMKLPFLAIPLGF
                     TAVKAMSERAFHRWLGIYVVVIFGTSLVVLINYLMHYETLNHDLTMGQPIPAPLGDHI
                     RFSLELAFAIICGGYLWWKRFYFFSPKEHHWWGIITIFLAVCIHVFAVRSGISALYLA
                     LLIVILHSAFVRKKYLAAGMGIVSLFVIGYCSATYIPSLKSRMDYFRYEITLIRAGDI
                     KVDHSDAQRLLSMEYGLQVARQNIFFGVGAGDIKTEMNKLYEQHAQSDLVKSKMPHNM
                     YIYFLAATGMLGLLVFLLSVLYPWLSNKRYKNLLFSVFLVMMLFAFMAEHTLEIQLGT
                     AYYLLFLLLIKKHIDDSNTPAYA"
     gene            22497..23120
                     /locus_tag="PLM90_01795"
     CDS             22497..23120
                     /locus_tag="PLM90_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ88127.1"
                     /translation="MSELATKSKRANFKPVENGNLTKSAKDRIFSKEFLPHMDALYNF
                     AFHLTLDEDDANDLVQETFLKAYRFIASYQQGTNAKAWLFKILKNGFINDYRRKTKQP
                     SKVDYDEVVSYHDTDDEGHVEYLDLRQELFQGMMGDEVTKALSELPVDFKTIILLCDI
                     EEFSYEEIARIIDIPIGTVRSRLHRARNILKEKLREYAQKEGFKEKR"
     gene            23123..23377
                     /locus_tag="PLM90_01800"
     CDS             23123..23377
                     /locus_tag="PLM90_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88128.1"
                     /translation="MDQQLPKHDCRNFVKKVFLVLDGELPEEERNLFIADIQRCKGCL
                     EHYHIEKTFKDFLSVKVERKECSNKLRDEIRNMVNNNPEA"
     gene            23525..24322
                     /locus_tag="PLM90_01805"
     CDS             23525..24322
                     /locus_tag="PLM90_01805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ88129.1"
                     /translation="MEPDYISINRKAWNDKVDAHVQSEFYNVAGFLNGETSLKHIELE
                     LLGDISGKRILHLQCHFGQDTISLARMGAYVTGVDLSDVAIAKAKELAATTGASANFV
                     CCNIYDLAQHISTPFDIVFTSYGTIGWLPDLKAWAALIRQFLKADGKLIFVEFHPAVW
                     MFDDDFKEIKYNYFNTGIIEETEEGTYADRDADIHNKTINWNHGISEVVQSLLDNDLS
                     ITGIHEYDHSPWPCFKHMESDGKESFYIKHIGKKIPMVYAITAERKV"
     gene            complement(24325..25500)
                     /locus_tag="PLM90_01810"
     CDS             complement(24325..25500)
                     /locus_tag="PLM90_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ88130.1"
                     /translation="MDKPSLHTIKGGLITTEQLLAYHKGELSEAECRRIEQLMADDPF
                     LADAIEGLQYATPEQVQKALDAIYAKVDARIAAGAKTGFGSQLRKYAAAATILVFLGA
                     TLIIMRELNKKTSESEIAFVESAQQHPAFNNADTIGMGAGDSNVEASTIEINRKSNVQ
                     PVVIENEFTASEATEDLSGDDKFSIDESINVSEGFSTVGNLDVVKPAVAEAYFRTNVA
                     VLSAIPEMSNGDFLLETKSIQEVAVLSDKKQVFKRKDEDKPNKENELTKDYSGAPAGA
                     SDTETMDDFQQGDSVMMADVQPQFVGGEKALVDYFNANLELPLSVSEGVVYVRFVVQK
                     NGTIANAYVVQGINLLPNENVLKAVNNMPAWIPGKVQNVPVDTWISLPITIGKKTDY"
     gene            complement(25493..26101)
                     /locus_tag="PLM90_01815"
     CDS             complement(25493..26101)
                     /locus_tag="PLM90_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ88131.1"
                     /translation="MFQLLFPRRRRPSEMTDEALIEAYKQSGDTSLVGELYKRYAHLV
                     YGVGLKYMKDPEEAQDVAMMVFEKLMVDLKQHQVQQFKPWLYMVAKNQCLMLIRKRKG
                     HETVELPEYNLENDEDGGMESGLTAHLDDDSDIKEAQLNRLEEGIKTLNEEQKICVEL
                     FYLQDKSYVEVAEITGYDLKQVKSYIQNGKRNLKIFLTKQNG"
     gene            26357..27925
                     /locus_tag="PLM90_01820"
     CDS             26357..27925
                     /locus_tag="PLM90_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88132.1"
                     /translation="MKNTTQNRASRLIQYSAATIGAILTQHADGQVAYTDLDPDVDLH
                     YITGDSYLLDINNDGIDDFEISAIVSSTVFTGSSSSSFTSYTYKVLVTPLLDNEVFLT
                     DDANFIEAGETIDADLTAGEFIPSAGDFNAVFFEVPSYIGLKLNTAGAINYGWLRLTN
                     DITSFTLLDYAVELTEDTGIYAQVPGGPHAVSNISTADVSDTHTAADVQVSFTIPDDE
                     SGTYSYRIFAGEISFSKMQAINADPEQYTEVFPTGSDQALTLNPGQLDYFHNPVEEFS
                     RIVVGVLSVSADNINESVYQSYIIPVVPGHDVEPVSNVEVTHVVSPYPGTSIEVNFTT
                     PDDLHGIINFLSYIVPADMLPIIDSSLIYINPNHAVLNAWEVGGGEFATDHSYEMPFT
                     GYDINGDVLTFGGQYGVCIQSHTQWPEYSGFAFESLTCSDTFTITDETIGISTMLSGE
                     LSANVVPSGIYLQSPQMMEAVELLGINGSLIYSSSIHADNTIIPLPEASGIYFIKARF
                     KDGFGIKKLNVVIK"
     gene            complement(27941..28360)
                     /locus_tag="PLM90_01825"
     CDS             complement(27941..28360)
                     /locus_tag="PLM90_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88133.1"
                     /translation="MKKYLIILISGMLFAACSEKTELTEENIAGVWNAKEFESTIPNI
                     PAEYQEAGKQEFLSSVYTLNKDNSFELRSSFFVNGAFGRWEFDPETRELSMFYEMDTT
                     HGVEKFNITALTATSMTLRQDIPSMSAFVQLELERSK"
     gene            complement(28357..29430)
                     /locus_tag="PLM90_01830"
     CDS             complement(28357..29430)
                     /locus_tag="PLM90_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88134.1"
                     /translation="MKSVLSFVIAIGVSAYMQAQSPITIVSSDLPDEGESFLVTNASP
                     LIAFDGTDTGPDHNWNFSTLVPVTQDTTNWIDENDTNPIYFFLWFTSDIAEQSVSNIV
                     NDFITITDVYNFYQLDNSTFAFAGFAGTIAGIPFPIGYDDNEVILNFPADYGQISNSN
                     TGFNISIPDIGGWNESRDRSNEIDGWGQVTTPAGTYDALRLHSVINIIDTFTYDTFVL
                     PVAYTTNEYRWLSKETGIPVLQINTQTVLGVETISSIVYFEDYYESAISEEQDLNNNL
                     QIAQPVVNEAMINFTATAAGNYQFRIFNLNGEIISTSDVNISIGENRIVINVDALPMG
                     TYLLQCNNEQGEMATKQFIKIAQ"
     gene            29478..30161
                     /locus_tag="PLM90_01835"
     CDS             29478..30161
                     /locus_tag="PLM90_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMZ88135.1"
                     /translation="MKWVTPIICFFVIGCSAPSVKQPQFKGGQMVEVVTLYETDTTGT
                     MFLWERSDEKRPWRCVDTINVSVGRNGLAPADTTYLNHKREGDGCSPEGIFPLTRVFS
                     YNVLNNLSMPFEQVDTNDLCVDDGASVYYNMLIDRDTAQINDYNSFETMHRKDRQYAF
                     GVWVDYNTPAIAGNGSCIFLHIWSSPGHPTSGCTAMAESDMLKLIHWLDAKQFPILYQ
                     HHLKPYENN"
     gene            30207..33368
                     /locus_tag="PLM90_01840"
     CDS             30207..33368
                     /locus_tag="PLM90_01840"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88136.1"
                     /translation="MKRFLFLAITSFFLLPINAQKNNKTTAAPATEVISKNYLSGMHW
                     RNIGPFRSGRCIAVTGVYNNDQIYYAGAVGGGLWKTSDGGHAWHCVSDSIFGSASVGA
                     VEVAQSNTNVVYVGMGEVEMRGNISFGDGMYKSIDAGKTWKKIGLEQSYAIGSIAIHP
                     QNENIVYVAAQGHIWGPNPDRGLYRSVDGGATWERILKGPNDNTGCVDVKLDPSNPNI
                     IYAGMWNAYRTPYSLSSGGPGSGLFKSIDGGKTWKEITKNPGMPKGMMGKCIVTISHI
                     DHNKLWASIENKDNAGIYASNDGGDTWFLLTKENDLIQRPWYFSNIYVDPQNDNELHI
                     LSVEYWRSINGGSEFSRAETATWDNHVMWINPTNANNFIIGGDGGPTITFDDGNNWSD
                     IDIPTGQFYHVNLDNDWPYGVYGAQQDNSSIKIKSRTSGYSIGASDWHPVAGGEAGYI
                     VPDPKDSRITYGGEYDGIFSSYNEANEVYRIISVDPEQHYGAGAITAQRRFNWTFPIA
                     FSPHNNSCLYATSNFVHRSYDAGVTWETISPDLTRNDPKTLQTSGGDITLDNTGVEFY
                     GTIFTFAESPVKAGVLWTGSDDGLVHVSTDNGTTWTNVTPSNLPEWSMISYVEPSYFD
                     AGTCYLSATRYKLDDMQPYIYKTKDYGKTWEKIVNGLPANMYNRCVRNDPNVQGILYA
                     GLETGVWYSLNDGAFWQPLQLNLPLAPVTDIRIEPKERDIVIATHGRSFWILDDITPL
                     YDLAKNGDAIQKSNAYLYPVNTGYRTPGPSYYAEEASDQTGENEAVGVKIRYYLKQKS
                     EKELRLAFYESDGDLIITYSNLYDKKRTPITIKDEFYPNPKVTESGKLNAFPGSHQFV
                     WDMRYPDATGDTSATFEASLMGPLAVPGNYMVRMYIGDSLVGQQTFVIDRDPRNPATV
                     ADLQAQFDMNQRICNKLNQIADATGSIRETRDMINEHIGEDASEEIKSKGKQLIDALT
                     VIEGELYNPKILAYEDNLKFPIMLEEKLAGLNYFLQMADTAPTKSMYAKYEDLNARID
                     VQLNALNKLMSTEVKQFTQMVGE"
     gene            33606..33926
                     /locus_tag="PLM90_01845"
     CDS             33606..33926
                     /locus_tag="PLM90_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88137.1"
                     /translation="MLHPEILRQILPGLVTSVAIRFPVAYIRSITFHVHEKMVAACFW
                     QQEEQRAAPFTIGENNMRSGCYIYNYDENIRESEVEITGMDESGKAFLISMPYNRIVP
                     MIAA"
     gene            complement(33927..35315)
                     /locus_tag="PLM90_01850"
     CDS             complement(33927..35315)
                     /locus_tag="PLM90_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HMZ88138.1"
                     /translation="MSGNLNTFLQKAEQKAFDAEHKRKLLFNIGKYNATVVQGKKQYA
                     DLEHVRKKVKNLKWKVMENLDKYLIEFESNFTANGGKVIWANDEKEAQQEILKILKKK
                     EARMAVKAKSMATEEIHLNSFLAEHGIESVETDLGEYIQQLDGEAPYHIVTPAMHKSK
                     DDVAKLFNEKLGTSLDLTAQELTLVARRNLREKYVKADVGFSGANFILPDIGGIAVTE
                     NEGNGRLSTSFPKTHIVVVGIEKMLTGVNDLALLWPLLATYGTGQKVTVYNTIFTGPK
                     KEVEADGPEEMYVILLDNGRSKLLAEEKQREALYCIRCGSCLNACPVYKNIGGHTYGA
                     TYSGPIGSIITPHFKGMEEFRHLSNASSLCGNCTEVCPVHIDLHTLLLYNRNEAVEEG
                     HGTFSEKMAWKMWRRSMLNRKSMDKPSPAIKNMGVNMLFKNSWGKRRAMFKFAPKSFN
                     KMWVEKNPPEDR"
     gene            35472..>35688
                     /locus_tag="PLM90_01855"
     CDS             35472..>35688
                     /locus_tag="PLM90_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88139.1"
                     /translation="MKQKILLLSVLLAIVPLSTYAGTTSNGPTFAGVPYEFILFGLTL
                     LGIALFHKYTLKIALSGLAAVLVYKIFV"
BASE COUNT        10512 a         7241 c         7445 g        10390 t
ORIGIN      
        1 ttttattccg cagtgatatt tgcacccttt ctatgtttaa tggaaaggcc agtagcgagg
       61 cgggttcgcg caaagacagc aaagaggcca aggcgcgaag tctggtatgc ccgcacacaa
      121 tcattgtctg aatcacggat tattgggatt aagggattgc acggattgcc ggctaagcac
      181 attccacaaa tcaacccgaa cacgaacaca tgaacacaca actcctcgtg ctctttgatt
      241 tcctgtgaag gacagtatga ttaatgaagg cttgctattg cagcggtaat catgagcggc
      301 tgaccattgg tgtcgaggcc ttctaccact acctgttctg cagcaaccgg tacatgcaga
      361 tcatcgtagc gcgttacggt tccgcgagga tctgactccc agttgcaatc ggcaatcttt
      421 ttgttattgc tgacaagtgc aatagaacgt atatacgcga tcggaaacga caacgccacg
      481 cgggtaacaa ggcctgcgag gaagatatct atatttctgt cggtgaacat acgtttaact
      541 tgctatacaa aaatgcaata cactagatcc tataccaaag acaaaatcaa cggcgtatcg
      601 ttacttgtat acgatattaa acgctatatg gattgattta agtatatggg aacttcaaaa
      661 cccttgttat ataaggattt caggagatga caaaatgttg ggggttatcg gaagtaaccc
      721 ggtcaaccga taaaattgaa agctgaccac ctattaaagt ctagaatgga ttctgataag
      781 cttcgttggt ttcgaaattg gtatccgtaa gtgttttcaa aaatgcgatg atagccgctt
      841 tttcttctgc cgtcaattga acaccacctt gatatgcaaa ttgaatgaga ttgtctgtaa
      901 aaggtgtatc atgcacgcct tcactgtaaa aatcgagcac ctcttccaac gtggcaaatc
      961 ttccatcatg catataaggt gcggtgagtg ctatatttcg caatgaaggt gttttgaatt
     1021 ttccatagtc agccgtatca ccactgatag caccaagtcc cgcatccgca aaatcggtgt
     1081 aatgtgttgc atcatcaagc ccgttgttgc gatatacata atccgtaaac aatccgccat
     1141 ttacaccatg acaatgaaaa cagtcggcac catccaatgc attaaataat gtaaacccaa
     1201 gtgtttcttc atcagtaaga aatatttctc ctgccattgc ctgatcgtac cgtgaatttc
     1261 cgctggtgac agatttaagg aattgaccta gtgcgcgtgc aactaattcg gtagtgatta
     1321 cttcttcgcc aaatgcttca taaaatgctt ttggataaaa ttcacttctg gtaatttttg
     1381 taattacgtt tggcaatatt tcatgcatct ccagcggatc ctgaataggc attaaggttt
     1441 gatcttcaat tgttgctgct ctgccgtccc agaaaaatgt tgcatttatg gcaacggcat
     1501 atcccaaatt aaaaatgggc atcgcattgc gctttcccgg aagtccatcg ataccgatgc
     1561 tgaaagaggt agtgtcgcta aatgcatgcg ccacgctatg acatccggag caggatagcg
     1621 tgctgtcggc cgacaatatc ggatcataaa acaacattct tcccagttca acacctttca
     1681 ccgtaagcgg gtttgttgta aacacaggca tgggtggaat accgtttacg tgatcagcag
     1741 tgtaaggcgt atcatcataa acccattcat catccggtgt aataggatca tccggatcgc
     1801 atttagtgaa caacatgata gttgcaaaaa atgcaattgg caatatccaa attctatgct
     1861 tgcgaaacat tatggtaaag gtaatattgt taattaaatt ttattccagc aaatccgggt
     1921 tttgcatgaa ggatatatcc gttaacgata ataaaaatgc ttttaaagct tccttttctg
     1981 caggtgtgat gctgaaccct gtaataaata cactttgatt ttcatgtcct gatccacctg
     2041 ccgcatacat gtcaataatt tcatctaacg tttctacctg accattatgc atatacggcg
     2101 ctgttacacc cacattgcgc aacgatggca ctttgaattt accaacatct tcgtgcaacc
     2161 atgtaatacg tgcgcgaccg gaatctgcac tatagtcgga aaagaaacca ttattttcga
     2221 aagtattatt cgtaaataaa aatccgctgt ggcaatttgc gcatcctaat gcatcgctaa
     2281 aaaacaaatc ctttccttga agttcctctg cagtgagtgc atgaatgttt ccttgatagg
     2341 catattgatc gtagcgcgaa tttccgctga tcatggtgcg ctcaaaagct gccaaagcgc
     2401 gtgttacgcc atatggatcg ggattggaac caaacacatc attgaatgac gtcaccattt
     2461 ccgcatcggt atttaatcgc tcgcaaagca ataccatatt aaatcccatt tcttcatgcg
     2521 tttcaatggg aacataaggt tgtgtttcga gtgaaggatt tccgccatcc attgtgataa
     2581 ccgtatacca tgcgacattt tgtaaagaag gtgcattgcg gaaacctgtt cttcccatta
     2641 caccgtgact gatagcaaga tgatcggcaa aagcattttc aggacgatga catgatgcac
     2701 atgagattgt tgaatcgaca cttaatgcgg tgctgtaaaa taattttttt ccgagtgcaa
     2761 tttttgcatc tgtcaggata ttatcttcag gaatttgcgg cacatcaaat cccggaggaa
     2821 tttgcaactc atatgccttt tcttcaggta tcacttcaga acaggatacc aatattgcac
     2881 tgagaaataa tatagaaatt aatttacgca tggtattaca taccattatt cttctccggg
     2941 cgagaacatg gtatatatat tatctaataa caaattcgcc aatgttacat tatctgtagt
     3001 gtgagtcgta ttatcgttta ccatatcaac accggtgaaa aagctggtgt aatctgcttg
     3061 caggtgaaca atattttcga gtccctgtcc aatactgatc ggcatttcta cttcgataga
     3121 cgcgaggtag tgatctgttc ccaaatgcat ctcaaaagca cctgcaggat cgggagtgcc
     3181 atcgccatca gaatccactt caccatcaat acgcataaaa atgtatccaa agctccatcc
     3241 ccaatgcatg cttggtgttt gtgcccccag agggtcgccg attggatata atgatgggtc
     3301 gccatggttg gttgcagagt ctataccaat atcaaatttt acagaagtat aatcgcctgc
     3361 gggaaaatct ccaatggcat actcttctgt ctccggttga acaaggaggt ataatccgtc
     3421 ttgtttaaat tcattgccat tagcatccac cagggtaatt ccgcttacat aaaattgcgc
     3481 caggttgagg ttggtgttta cgccgttaat gacataaggc atttcttcct ccagttcaat
     3541 atcattgaga aactggtgca catgcatttc tacggcatag gtttcttcat acagacagcc
     3601 aacattcttt tcggcatcgg gatcgtagtt ggtagcagtc gggtcagtac aaccttcttt
     3661 tttgctgcaa gaggaaactg ttaacagtgt gcagatcgcg aggagggcag atccaaaatt
     3721 tcttttcata gttgacgttt cgggtataat ttttgtaaac taatgttcaa agttagccgt
     3781 tattaccaat gaaacgctga cttttgtcag ttcgtttggt cggaaatggt caaaatgctg
     3841 tcaaaacacg taattttgca acaatgaagc gtctgcgcgc catatttggg atgatgatgc
     3901 ttgtgctcct ttttgggata acaagtggtt tcaacctgtt cacccacctc tgtctgatga
     3961 ccggggagtc gggtgttgcg cctgtttcta tgtctatgga ttgctgtggc aatcagctcc
     4021 cggatgctcc ggtgaccctt tctatgaact gctgtattga aaagacgcag tttatcaagt
     4081 ttgaatactc gggctcagtt caaaatcagg ttggctatac cgactatgca gatttggact
     4141 atcctgtaat tcaaatacag gatgaaattc aggctgatgt agtcaacctc aactccttta
     4201 acctgccacc tccgtttccc ggcgatatca gggtgatgca gtgtatctat cgtatttgat
     4261 gtgcaatgaa aaatggtgaa cctgccaaag gggcgggaaa tttttcattt caatcaatta
     4321 cttatgatat caataaataa aaattttagg acctacattc tttcatcact gctttttatc
     4381 tgttcagcat ttatggctgc cccggttttt gcccaacaaa tggttgaagg cgaggtgatg
     4441 acgaacgacg acaaaggaaa aatgattccc cttccaggtg caaatgtgta ttgggagggt
     4501 gaatattccg gcgttatcag cgatgatgaa ggccattttt caattgaaca aaaaacaaca
     4561 tcacaacgct atctggttgt gagttatgtt ggattttcag ccgatacctt tgatgtgcaa
     4621 acaactaaac atatacatgt gatgttatcg cctgatacag aattaaaaac tgttgttgtt
     4681 gaaggcgcac aatcgtcaaa aacaattaac acggtcgatc ctatcggcac agaacaactc
     4741 aataaaaaag aattgctaaa agcagcatgt tgtaatctga gtgaaagctt cgaaaccaat
     4801 aataccgttg atgccgaatt cagcgacgcc gttacgggag cgaaaacaat taaattgctg
     4861 gggctcgatg gcgtgtatgc acaaataatg acagaaaaca tggataatgt gcggggattg
     4921 tcttccgctt acggtctgac atttataccc ggtccatgga tagaaagcat acaaatcact
     4981 aaaggcccgg gctctgtagt aaatggctac gagggcatca caggctccat caacactgaa
     5041 tacaaaaagc cgtttgatgc cgatgaagaa acagcacgtc tggatgtata cggcaatcaa
     5101 tttggcagat gggaagcaaa cgctgtctat aagcatcctt ttaatcttaa atggagtaca
     5161 ggattatatg gtaatgtttc gcagatgcaa caaaaagtag atcataatga tgatggattt
     5221 ttagatgtac cgatgaacag tacttacact ttcatgaata agtggaatta tttttcgggc
     5281 ggcatgcacg aggcgcaatt tggcgtgaaa tatatcaaca gcaacgttac cggcggacaa
     5341 acctcatttg atgttaacag tccgcggacc acagaaaacg gatatggcgt aggtgctgat
     5401 attacgcgat gggaattatt catgaaaaat ggatttgtgt ttaaacgtcc ttctacatct
     5461 ataggcacta tattgcaatt tgccgatcat aatcaaaccg gtttttatgg attgaataat
     5521 tatgccggtg cggaacaatt tattaaagcg gaagcaattt ttgttaccta tatcggcaat
     5581 accaacagca ccataaaaac gggattgaat tatctgtatg ataatttcga tgaatcattt
     5641 gattccatat cctatctgcg caacgaatcg gtgcccggaa tttttgcaga atatcattat
     5701 cagtttgaaa aaaaatggag tgtattggcg ggattgcgtg tcgatcaaca caatctctac
     5761 ggcacttttg taacgcccag gatgcatgtg aaatttacgc cgaaggaaaa caccaccttg
     5821 cgcgcttctg ccggaaaggg ttatcatata gccaatgtgc tgatagaaaa cagcagtatc
     5881 cttaccagca atcgcaccct catcattcag gaaccattag agcctgaaga ggggtggaat
     5941 tatggtattt ctttgatcca gcagtttaag cttaatcaca gagagggaac attcaccctg
     6001 gatgtttatc gcactgattt catcaaccag gtggtgatag atatggatgc taatgtaaat
     6061 gagatcaatg tctacaacct taagggcgaa gcatatacaa atgtgcttca ggctgaaata
     6121 aattatgagc caataaaacg tcttgagttg cgcgctgcat ataagtatgt agattcaaaa
     6181 gcgacttatg acggaaagct tttagaagtg cctttgacct ttaaagatcg cggcttattc
     6241 aatgtcgcct ataactggaa aaaggcggga ctttcagcag acgccacggc tcaattctac
     6301 ggcaaaacgc gcctacccga cctgtcagaa aaccacgcag cacatgactt tggcacgaat
     6361 tctgagcctt atattcaact gttggcccag gtgacaaaat ctttcggcga cctggatgtt
     6421 tatgtcggat cggagaatat taccgggtat acccagcaca atcccataat tgatgctaca
     6481 gatccgtttg gtgaaaactt tgatgccggc gtcatttatg ccccgctgat gccgcgtatg
     6541 ttttacggcg ggttgagata tatttttaac taattgataa aaaaattaac tttgaatatt
     6601 atgaaaaaga ttcttgcaat tatcggatta ttcatggcta taggcatgca aacgtcattt
     6661 gcacaaaata gcggaaaaca atatatcgaa attaaaactt cggcagtgtg cggcacgtgt
     6721 aaggctgcta ttgaaactgc tctggaaaaa gttgatggcg taaaatcagc cgacctggat
     6781 ctggaaacaa aagtagtaag cattaaattc gatggcgata agacaaatgc cgacgcatta
     6841 aaaactgcca ttacagaagc cggttataca gctgatgagg tgcctgcaaa taaggtagcg
     6901 tatgacaact tgcatccttg ttgcaagtcg gaacaataaa gacacaatta caaaaacatc
     6961 aaatgaccgc gggaatcatt cgtgatcctt cggtcatttt ttattttgcg ccaatgatat
     7021 ccaaaaaaga tttcttgtag gtctcagata tagggagtcg ctttccggca atcaaaaccc
     7081 gatctttctc aattgaagtg attttggaga tggaaaccat atacgattta tgaatgcgac
     7141 atatcttttg gggtggaagg attttttcga attcagtaaa agtttgcaat gtcattatgc
     7201 ttttgtccga aagatgtacc cttcgataat cgcgcattcc ttcgatatac tgaatatcgt
     7261 caagcatcac tttttcaagg cgatgttctg ttttcacaaa tatgaatgct ttctcttgcg
     7321 tcacaaccat ttgtgtcccc gcacgagctt tttcaacagc ctgataaaag cgttcaaagg
     7381 tgaagggctt catgaggtaa tccgtaacat tcaattcaaa tccctttaga gcatattcct
     7441 gataggcggt ggtgatgatc accggacaat ttggctttat cacctccagc aattgtattc
     7501 cgctcatttc gcctagatta atatcaagaa acaacaagtc tatatgatgc tcgcgcaaga
     7561 agagcaaggc gtcgatggca ttgtcaaatg atccaaccat ttccagaaag ggcagtctgg
     7621 cgatgtattg tttaattcgc tcctgagcca atggttcatc ttcaatgatc agacatcgca
     7681 tagttaaagt ataattttaa ggcttacgca atattgttcg ttgtcagtgg ttaactgcaa
     7741 ggtatgtgca ttcgggaaca ataattccaa tcggcgacga attaatttat cgcccaaacc
     7801 accatttaat gtatcggcat cttgtatttt atcaggaaaa tagtgattac agcaattgaa
     7861 aagcaaagaa ttgttttcaa taatcagctt aatattaatt gcgggtgttt tctttttatc
     7921 gcccacatgt ttaaaagcgt tttcgataaa agggatgaac aacattggtg gtaccatata
     7981 ttcagaggga tcgccgataa cttcgaaatg cacgtaatcg gtgttattac ttcgtatacg
     8041 atgcagatcg atgtattttg tgatataggc gatctcgcgc gacaataaga tgcgatccgg
     8101 ttttgactca tataacataa atcgcataat atccgataag cgattgagat attcagaggc
     8161 cttttccggg tcttttgata tcaatacgtc gatattgtta atggtattaa acaggaaatg
     8221 cgggttgatc tgacttttga ccaaagacaa ttccatttcg gtgtttttgg cagcgagtga
     8281 ttctttgata cggatatcac cataccagga gatgaagccc cgcataacca atcctattac
     8341 gccgtttagc atggcgacaa atccaaagaa aacgacaatc gcaatcaaat ttcctatgct
     8401 catattggcg atataatatt catcaccaat gagccaaggt gcagcgcccg aaaacgcacc
     8461 caccacgccg catattaacg ctgttactat caccgcaata aaaaactgcg ggattttacg
     8521 tttttggaga tatctgttaa aaacaaaaaa atagtttaaa taaaagcaaa ttacaccggg
     8581 taaaatcgtg gtgctggtca taaaaccgag ccaatttcgc aataccggtt tatctgattc
     8641 gtcggaagtt ggtgataccg atgatattaa tccgaatatc accaatatca gtagcatgaa
     8701 taccagccaa tatccaagat tcaataatac gactacgtac tttttcattt tccggactta
     8761 aaggtaatat attcggttat gttacccttt ggcttttctg gtcacataca acacatatcc
     8821 gaaggtcaaa aaaacgataa aataacccag gcaccaggca tagatatgaa ttttttctaa
     8881 ttgcgcagca tctcccatga tagttaatgc gggatatgaa taaggaatag tataagcata
     8941 ttcccattgt ccggtcagaa ttaatgagcc aaccaccagc aataagccta tgccgatgga
     9001 cacaaggaaa tttttaaagt ggaggcttaa taaaaattga agcgccaata caggcaaaca
     9061 tgttaacaat atttttccgt tacttgtcaa aaattgattc caaggaaaat caacaggagg
     9121 aaaatgcccg gcaaaaatca gcgaaggtat tgctgcagaa agataccagg tggcattaaa
     9181 atacaagaaa aaaagaccgg tcatacccaa caatacgata aacttagcca taaaaattgc
     9241 cgtatatgac tgcggggtgg tgtgcaccgt tttccaggtg ttatttctgt attctacctg
     9301 agtgataaga cttgttgcca atatcaaccc catgggcaat aaaaacacct gcatatattg
     9361 ccatacctga aaaaagtaat aggtccatgc attgagttcg ccctgataca tatcgatggt
     9421 cctatgatct ttcaacatgc caatcagaaa aataagtgga ataaatgtac cgccgattaa
     9481 acaaagccat gtggcaatac tgtgtttcgt ttttaaccat tctgatttaa tgctttgtag
     9541 tatcatcatc ttcatttaat aagttccata aaaattgttt ctatatcatg tttttgtgct
     9601 tcgattttaa atacacctac acctctttca ttaagtgtgc gattaagttc aacaacacgt
     9661 tgaatatttt cgacagacac gtgaaaaaca ttttcggcaa tatgataaga tattccctgc
     9721 tcatcaaata tttgcttcga taatgtaata ttatctgttg cgattagggt tcctttttga
     9781 tgtctttgca agtcagccaa ttcagtagtg ctgccttgaa atcggagtat tccattgtga
     9841 ataacaccca catgcgttgc cagtttttca atttcggcga gcaagtgact tgacaccaga
     9901 atggtcatgc cattttcttt atgtaatcgc aacaacaact ccctgatttc aatcatgcca
     9961 tttggatcga ggccatttgt tggttcatcg agaattaata tttcgggact atgcaataag
    10021 gcgatggcaa tacccagtct ttgcttcata cccagcgaaa atttcccggc cggttttgaa
    10081 cctgtttttt ccaatccgac taattgcaat acttgttgaa tacgcttcct atcagttccg
    10141 aacaatattt gctgtatgcg aacattttcc gttgccgaca aatgtgcata aatagacggc
    10201 gactcaatca gatttccaat gctacgcatc aacaacgttc tgtgtttatg caaaggcata
    10261 ccttttatat aaatcttgcc ttgttgcttt tgcagcagtc cgagtatgag ccgcaatgtg
    10321 gtggttttac cggcgccatt aggcccaaga aagccgtata tcgacgcccg ggggatgacg
    10381 agatttacat cctgtacaac aagctcattc gagccaaatc gatgtgtcag gttttcggtt
    10441 acgatacaat tttccggtgc agacatattg aatgtttttc caaaaatatc tccaaccggt
    10501 gccaggctta aatgaaattg gtgtttgaaa ggatactatc tgcaaaccgg cctttttatc
    10561 ctgcaagatc aaaatcgaat gtatagtgtt ttggaatgat acctcaatta acaacctttg
    10621 gtacagatcc gataaagtcc cgcacaattc catatttcaa tcgctccggg tcatcaattc
    10681 gtttttgcaa aaaataaggc ggaacaaact gtccggaatt aaaatagcct tcatacggtt
    10741 gggaaacgta tatgtcttca ccgattttca atctgattcg aagcattcct tttgtgtcac
    10801 cttgaaattt tggcgcaacg atcatcacaa attcctttgg atgcacttta atcccatagt
    10861 agccattacc acagaaatca tatggtaggc attctatcgg ataccattgc tcattgtgtg
    10921 gatccattgc ttcctgaata gcaaatacgt aactgtcttt cgcaacaaac acttttgtag
    10981 attgtgttgg atttacaata aataccggaa aacaggcata aggagtaagg tcgtcgccat
    11041 agtaatcata cagggttgtt gtataatcgg taattatttg aaagccatga ttattggtgg
    11101 tatccaacaa ttgagtcaac atactttctg ctaccatatc gcttttaaac ttaatatatt
    11161 tcgtgtgcgg atgctttcta taatatattt catcattaaa cctgtatttt tcaacgatcc
    11221 aataatttgc aaaggacatg gtatccataa gcaaattttc aaggggtgta tttgttggat
    11281 gttgtattac cttcggtgtt tcgaaaatat cagtgcaagg tgttgccacg ttcggctttg
    11341 ttgtacatgc atgaatcgat ataatggaaa tagcaaatat tggcaacttg aggtttttca
    11401 taggcaagat ataaacgtta atttttccag attaaatatg attccatgaa agaattttga
    11461 gaagccggag ttatatggct actttcgcct tcaacaaaat taaatatcac attatggcac
    11521 aaaagatcac cacctttttg acctacaacg accaggctga agaagccgtt gaattttatt
    11581 gcagtatttt tccggatgcc aagatcgtct caaagcaata ttatagcgac tcgattcccg
    11641 gtttaggcgg gaagttaatg tctgcaacat ttactttatt cgaccaaacg tattatgcat
    11701 tgaatgccgg gccacatttt cagttcactg aggcaatatc gctattnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnatcg ctattggaat gcgctgactg aaggcgggaa
    11881 agaagatcgt tgtggatggc ttaaagacaa atatggtgtt tcatggcagg tgataccaaa
    11941 acaacttggc atgttattaa gtcaccccga caaagcaaaa gcctcgcgtg ccatgcagtc
    12001 gatgatgggt atgcgaaaaa ttgatatcgc cgaattagag cgagcatcag aaggttgaat
    12061 ttttttgaaa aatatacatc gttttaaata aaaaaaggtg tgttgaaatt tccaacacac
    12121 cttttttatt atatccttta tctcaatgta gaatttgaat atttaccgac tgcactgaat
    12181 tttcatctac aacctgtgca atatacattc cggaaggaat tgttgcgcta gaaaatgaaa
    12241 tcattccttc cgatatggtt ggattttcaa tttcgctaac attattaccc aacaaatcgg
    12301 taagtataat tttttttgct gatgcagaaa caccgcttac atgcaccata tcatttgcgg
    12361 gatttgggaa tgcctttaat gtggtttgcg cagtttgatc tttgatacca actgtactat
    12421 tgctttcatt gatcatggcg acttgcgata tcgttggaaa accgccggtg cttacacttg
    12481 aaaaataata ggtaaacaat ggacctacca caccttcttt cagccaaaac cagatataca
    12541 tgtcataatc aatatctacg ggtactccaa cggttacatc ggaataaacc tcatgcacaa
    12601 atacgcgcat cacatcggtg taggtgccgt caggaagttt cagggtacca tatccatcag
    12661 ccactgtttc tacagttccg ctgcggatga tttcaacccc cgcgtcgata gtgctataaa
    12721 agtcgtcgct attggtagca ttataggtta tcgggaatgt catcagctct tcaggatcag
    12781 aatataatac cgttacatcc ggtgtataga tgccatgatt ttcgtaaacg gatgcgttga
    12841 ttttataaaa actatagttg cccataccat tgtccgcggc tacgttggca tcagggaaag
    12901 ctgcactctg acctgtagcg gctgcagtaa tgaaagcata atcattggtt tcgccggtga
    12961 gggttacccc gctgaaatcc caggtaacat cagcaccact tgcgccggga tcaaaaagta
    13021 tggcgtcgat cattttattg gtaaactcgt cgcccgggcc tcctgctgtt gccgaagtaa
    13081 tagtaggttg tgcctgcatg agggctgtag ttgcgaacag gagcatgaga atttggaatt
    13141 gaaggttttt catttgatag gattgtaggt taaaaatacg aatccctttg gggagtggtg
    13201 attttttagg acactttaac tataatttta atatttcaag cctttccggt aaagggtttc
    13261 aaccgaattg cgcgcttttt gacagcggtt tgataagttt taaccaaaaa ggcgtgcttg
    13321 caggtgaagc aaaggcctca aaccccttgt tttcgtgtaa aaaacgcagt atctttgccc
    13381 tccggttcgg tcaaatccgt caattcggga aacttttatc tttagcattc cgttctattt
    13441 atcggttcaa gttcatcact aaagcaaaag aggtctgaag agaaatgcgt cagcttaaga
    13501 ttacaaaatc catcaccaat cgcgagagcg agtcattaga gaagtattta caggaaatcg
    13561 gcaaggttga ccttgttacc gctgaagagg aagtaactct tgcgcagcgg atcaaacaag
    13621 gcgatcaact tgcgcttgag aaactggtaa aagccaacct ccgtttcgtg gtatctgttg
    13681 cgaaacagta tcagcacaat ggtttgtcgc tgaacgacct gattaacgaa ggcaacgttg
    13741 gtctggtaaa agccgcacag aaattcgatg aaacgaaagg tttcaaattc atctcctacg
    13801 ccgtatggtg gatccgccag agcattatgc aggcattggc tgaacaaagc cgccttgttc
    13861 gtttgccatt gaacaaagtt ggttcattga gcaagatcaa ccgcgcattc agcgagcttg
    13921 aacaaaaatt cgaacgtgag cctacacctg aagaacttgc tgaaatcctg gacctggcac
    13981 cggatgaaat caaaaataca cttagcgtat ccagtcgcca cgtatctgtt gacgcaccat
    14041 ttgatgatgg tgaaaacagc tcactgctcg atgtattgga aaaccgcgat gtagaacgca
    14101 ccgacgagaa actggattac agccactcgc taaaagtaga aaccgagcgc acgcttgcaa
    14161 ctttaacaga gcgcgaacgc gatgttatca aacttttctt cggtattggt gttccatatg
    14221 caatgacatt agaagatatt ggcgaagaat ttggtcttac ccgcgaacgc gttcgtcaga
    14281 tcaaagacaa tgctatcaat aaattacgtt caagcacacg caataaagca ttaaaggctt
    14341 atcttgcgca gtaattaaaa tactgtttcg aaagaaacaa atatttgtgt tttcatgggg
    14401 atcccccggg aaagtcttcg acggaaccgg gggttttttt atgcccggca tttaggattt
    14461 ttcagaataa taaattgaag ggcaatgcca attacttcca attttttact tcacaaattc
    14521 tttcatacgc ctcaatcacc agtcttaatt ttgattccaa taatttcttt tcgtcagcag
    14581 aagcttgcgg atttgaatca ggatggaatt tttttgcaag gcgataatag gcgcgtttga
    14641 cttccttttc atttgctgta gaagcgagcc ccaatgttgt gaatgaagat gataatgaat
    14701 tgaattcttg ctccggacgg tgagcctgtt cttcataagt ttgttcttca gaatattgtt
    14761 gatcgcgcat ttttcccttt actgtgttgt acacctgctg caccgtttca aaaggtactc
    14821 cgattctggc agcgagataa ttaatgctgt gatgcagatt ctctttgttg acaccgggtt
    14881 gttctccaag ttcgaataaa aatcttagta aagtattttt ttctcctaca gaagcatatt
    14941 gttgtatttg tccgcaaatt gcttccactt gaatacgctg tcttaataat tcataaaaaa
    15001 aactcagtct tcgctgcgta tatgtttcac caaaagtatt taccaaagcg ctgcgcacca
    15061 cgttacttga caatttactt cctacgccgg gtgtttgcat aacaaaggct cccagcatca
    15121 taaagctcac gcggaaatca cgtttttcct ttttctcagg aaagacatat tccgtatcca
    15181 taaaacatcc gataataaat ccgactatgg ctcctcgaag ttcaaaatcg agcagtaacc
    15241 cgaataccat caacgcgaat ttattcaaac cgaaaacctt caaagtcatc ttgactccgc
    15301 tttgcgtgtg aaaggtgatt ctgaggtttt tattgaattt caaattatcg tgctttactc
    15361 gtgttcggcg atccttttat tctgaaacgc catggaatgg ttttgtacgg accactgaaa
    15421 ttcataccca cacgggcacc ggcaataatg tcgttgtcgg gtaatacagg tgcatcagtt
    15481 atccaaatgt gttcaccaaa taacgacagc ccgctatgct ttgttgttaa tccgaggcat
    15541 tgcgcaacta agccgggtcc tgcagccagt tgtggtgtgc tatcggatag cccccttcgc
    15601 ttcagcatat ggttgatacc cccaaccggt gcaatggctc gaatcaacac tgcatgcggc
    15661 tctccggtag tatgcgttac cacattaaac aggttgtgaa tgccatagca taaatataca
    15721 taagccactc ctccttcggc atacatgatg cgcgttctgt cggtcagtcg gccattgtat
    15781 gcatggcagc cccgatcttc taccccacaa tatgcttctg tctcgcaaat gatgccggat
    15841 gtatgtgagc cctggaattt agtgtgtagt gttttaccca aaagagcacg tgcaatacct
    15901 accgtatcgg tctgtgtgaa gaattctttt tttaatacgg tactattcat gcaaatttgt
    15961 tgagccgcag acaaaaatac acctgataaa acaaatggcc tcattatatt taattccaac
    16021 catactcagt gaagatgcat tgcataccat acctcagtat gtgcatgaaa ttacattgcg
    16081 catccgcatt tttattgttg aagacgaacg cactgcgagg cgctatttgc gcaaaacagg
    16141 ttattcggta tcgtttgatg atgttacctt attgcctttg aatgtcaaca cagctccgga
    16201 ggagatttcc gggtatatgc aatatttttc aggagataca gaaataggat taatgagcga
    16261 ggccggtgtg cctgcagttg ccgaccccgg aagtattatt gtgaataaat gtcataccat
    16321 tggtacacgc gttgttccac tggttggacc atccagtatt ttgttggcat taatggcaag
    16381 cggaatgaat ggtcagcggt ttgcgtttca cggatatatt ccgttaaaaa atccggaacg
    16441 aaaaaatcaa ttgcaagcga tggacattgc tgcgaaaaaa ggcgaaacac aaatatttat
    16501 tgaaacgccg tacagaaaca atcaattgat acaggaaatt atttcttcat gtcatggcga
    16561 tacattatta tgcatagcga cagatattac cggaactcac gaaaaaatta aaacactacc
    16621 cgtttcggca tggaaaaaaa acatgcctca attagacaaa acgccaaccg tatttttatt
    16681 aggcactgct tccaagtgaa atgctccttg tatggaagtg atgttttggc tcaaatcggt
    16741 tcacaattga caatattttc actgaggaat aattgtattt ttgcagtatg atcatcattc
    16801 aacaaactct tgtaagcgac gaaattcttg acgaaaaatt tgcatgcaac ctgcaagcat
    16861 gcaaaggcgc ctgttgtgtt gaaggtgatg agggtgcttt tgtagagccg gaagagcgca
    16921 agatgctgga aaaggtttat cccaaggtaa aaccctatct cacaaaagaa ggtatagacg
    16981 gaattgaaaa acaagggctt tgggtaacca acagcgaggg tgttctaaaa acccctttga
    17041 tgaaagacgg tccttgcgta tacacgatct gggaaggtgg aattgcactc tgtggaattg
    17101 aaaaagctta tctcgacgga aaggtgaaat ggcaaaaacc gattagttgt cacctctacc
    17161 caatccgatt gtccaaagta ggtgaatatg aagccttgaa ctacgaagaa tgggatattt
    17221 gcaaaccggc atgtaaaaat ggcaagtcgc tgggcatacc ggtgttcaga tttgttaaaa
    17281 ctgccatcat ccgtcgctac gggcaggaat attatgatgc gttggaggct acttatgcct
    17341 acagaaaagg cgaatagtgt cctcgcggcc atcaccattt aagtattgct ttcatcagac
    17401 cttattacct ttgttatatg cataaggtcg actttcttgt catcggatcg ggaattgcgg
    17461 gattaacgtt tgcattaaaa acggcgcgaa aatttccttc tcgcacaatt gccattatca
    17521 ctaaagctga agaggacgaa acaaatacaa aatatgctca gggtggtatt gccgcagtat
    17581 gggattttga aaaagacagt tacgagcaac acattaacga caccttgatt gcgggtgacg
    17641 gattatgcaa gcgcgatatc gttgaaatag tggtaaaaga aggacctgaa cgcgtacgtg
    17701 aaataattaa ttggggtgcc gatttcgata aaactaaaga aggcgaatat gcgcttggca
    17761 aagaaggtgg tcattcgcag ttccgggttt tacatcacaa agatgttacc ggaaaagaaa
    17821 tagaaaaaac attgttggaa gaagcacaat cgcttccaaa tattactttt tttacacatc
    17881 atttcgttat tgatattatt acgcaacatc atctgggaag aattgttaca cgacattcgc
    17941 cggatattca atgttacgga gtatatgtgc tcaacaagga aaataaatcc atagaaacgg
    18001 tattatcgcg tatcacagta atggcaacgg gtggtgcggg acaggtgtat aaaagcacaa
    18061 ctaatccgct tattgctacg ggcgacggta tggcaatggt gtatcgcgcc aaaggtcggg
    18121 ttgcgaatat ggagtttgtg caatttcatc ccacatcatt atatcaaccg ggagtatcac
    18181 caagtttttt aattacagaa gcgataagag gtgcgggtgg tgttttgcgc aatagtaacg
    18241 gtgctttgtt tatgcagaaa tatgatgctc gtgaatcatt agcgcctcgc gatattgttg
    18301 ctcgtgccat cgataatgaa atgaaattgc gcggcgagga ttttgtttat ctcgattgca
    18361 caccgatagc aaaagatgaa ttgctcaatc acttcccgaa tatttacgaa aaatgtaaat
    18421 cgatagggct tgacattttg aaagatccga ttcctgttgt tcctgctgcc cattatatgt
    18481 gtggtggtat acaaacagat gctttcggac attcatcgat tcaatattta tatgcctgcg
    18541 gcgagtgttc gtcgaccgga ttacatggcg ctaaccgact ggcaagtaat tcgttgttgg
    18601 aagcattggt ttttgcaaat cgcattttta ttgatgcatg cgaaaaaata gatggcattg
    18661 cgcttgccga tcaaattccg gattggaatg ccgaaggaac agatgatccg aaggagatgg
    18721 taatgatcac acaaagtgtg cgagaattga aagaaatcat gaccaattat gttggcattg
    18781 tgcgcagcaa cgagcgctta gaccgtgcat tgactcgctt gcatctgctt tacgaagaaa
    18841 cagaaaaatt atacgagaca actacactct ctccgcaact ctgtgagcta aggaatttaa
    18901 tcacggtggg tttcctgata acacgcggcg ccgccatcag aaaagagtcg cgcggattgc
    18961 attacaccac cgactaccct catcacagca aatgggctga agacagtatg ttttaaagat
    19021 gcgggagtaa ttttaatgtc gtttttttaa catttacagg ccgtttggcg taaaatttgt
    19081 attgatagca taggcatttg ctacctttgc atgatgcgtg tgtttctatt tgcattgtcg
    19141 attttgtgtg ttacgcattt tactgctgcg caagattact gttcgatgga tcgtttcgat
    19201 acctatttgt gggcagaatc tgatatagaa gtatttacaa atcttgaata tgggcaagct
    19261 gtaaataact ccggaaacaa cgagcatttg tttctggact tatatcatcc acctgttgca
    19321 gaagatccat taacatataa gccttgtgtt ttttttgttc atggtggtgg tttagttggg
    19381 ggagataagg acagcgaagg tgctaaagat ctgggttatt tgtatgcacg aatgggctat
    19441 gtttttgtga gtatcgacta tcgtgtggga tggaataatg gcgatgcaga tgatggttgc
    19501 ggcggtgatt cgacagatct aaggcgggca acttaccgag cagtgcaaga cacaagggcg
    19561 gcataccgat ggatcaaggc ctacggacca ttatatggtg tagataccaa taacattatt
    19621 gttgaaggaa atagtgccgg ttcaatgtta ttattatacg cactgtatgc cgaccaggaa
    19681 gattatgacc caatgtttta cgacgaattg ggaagtatag attcagcagt aaatgagtta
    19741 actaacttcg gatttgatcc aatcgggtgc attacagaag ccgcaggtat tgaggataca
    19801 acactactgt tgataaaaga tgtcccacat ttattttttc acggcacctg cgattctata
    19861 gttccatttt ttcatgggcc aacatttttt tgttttcaac catttccata tccaaccatg
    19921 aatggctcat ggccaattgt gcaaatgttt aaacgcaatg accgtaacta tcacttttat
    19981 attgctgaag gcgccggtca tgatgcggta attcctgatt ccatcattgt ttattcggct
    20041 ccattcatta aggagatatt atgcaacgcg tctacgacaa aagaatttta tcgcgtgttg
    20101 ggcaaattca aatgcgcggt ggccaatgag ggggaattag ttatcacgga tgtgtaccca
    20161 aatccggtgg ttaataccat atatcttgag gttacgagca ctcggcaacg cgaaattacc
    20221 atcgacgttt ataataatct tggacaatcg gtgctttcgg gagaacttcc ctttacgccg
    20281 ccaatcggtg aatacacaat agacctatcg catttgacaa gaggtattta ttatatgcgc
    20341 ctcagccagc gacaagaaga atatgttttg aaatttgtga aatattaagc gcgcttcatc
    20401 acttactttg catctttctc attccctcca ctttcacata tcgcataaaa acagctttcg
    20461 cacttagtga tgaaatgatg tatccggcct tgccatcaag aatgcctagt tgcaaaaaat
    20521 aatgtttaat aaatcggaaa gcaggcttga ttacgaagtg ataaaatccc ggattgacat
    20581 ttttcttcat agcgtcctgt gcgctccagg tagtgtagcg atcccatttc tccatgtaat
    20641 ggaaaacatc tttgtatgtg tgatgatcaa gcttcgattt caagtgacca atttttccgg
    20701 aagtaacaac ttctgcatga actgactttt cctcatatcg acacgtatct cgattaaaga
    20761 atcgtataac tttatcgccc tgccagccac tgtatttaat ttccttaccc ataaaaaaat
    20821 tgcgcctgct gatccaatat gcatcacaag gtatttcaga ttttttcagg agtgctgtta
    20881 tctcattacg tagttcgccc gttacccttt catcggcatc tactaataat atccattgat
    20941 gcgaggcatt gggaatgttt cgatttttct gactggcgga attaatatat tcactttgta
    21001 tgatgcgcgc gccgagacgc ttagcgcgtt cgacagtatc atcagtacta aatgagtcaa
    21061 ctaccaatat ttcatcacac cactgtacac ttcgaatcac cgcatcaatg ttatgcgact
    21121 cgttaaatgt tggtattaat gcagtgatct tatgcataag cgggcgtgtt actatcgtcg
    21181 atatgttttt tgatcaacaa caaaaataag agataatatg cagtacctaa ttgaatctct
    21241 aaagtatgtt ctgccatgaa agcaaaaagc atcatcacca aaaaaacaga aaacagtaaa
    21301 tttttatagc gtttattact tagccaggga tataaaaccg ataaaagaaa gaccagcagt
    21361 ccaagcatgc ctgtagccgc taaaaaatag atatacatat tatgaggcat cttagatttt
    21421 acgagatcgc tttgggcgtg ttgttcgtat agtttattca tctcagtttt aatatctccg
    21481 gcacctacac caaagaaaat attttgcctg gcaacctgga gtccatattc catgcttaat
    21541 aatcgttgtg catcgctatg atcaactttt atatcgcctg cacggatcag ggtgatctca
    21601 tatcggaaat aatccatcct tgatttcaga gatggaatat aggttgcaga gcagtatcca
    21661 ataacaaaca aggagacaat tcccattccg gcggccagat atttttttct gacgaaggcg
    21721 ctatgcagaa ttacgataag caaagcgaga taaagtgccg atatccctga ccgcacggca
    21781 aacacatgta tacacaccgc caaaaaaatg gtaatgatgc cccaccagtg atgttctttc
    21841 ggggaaaaaa agtaaaacct cttccaccat aaataccccc cacaaatgat ggcaaaagcc
    21901 agttccaggc taaaacggat gtgatcgccc aggggagcag gaatgggctg acccatggtg
    21961 agatcgtgat tcagcgtctc gtaatgcatc agataattga tcaacaccac caatgaagtg
    22021 ccaaaaatga ccacgacata gatgcccagc caacggtgaa aagccctttc tgacatagct
    22081 tttacggcag taaacccaag cggaatggcc aaaaacggga gtttcatgcg gcagcggtcg
    22141 acccaatagc cgatatcgct gctccaaaat cctgagaata ggtacaccag gaagagggca
    22201 gcacaaagca tggcagtagt atcggagaac catttacgaa aattgctacc ggccctgata
    22261 ttgataaagg cattacacat gagggtgata gttgccacac tgaggaccac ttttgaagtg
    22321 actaggccga caataagtcc gagaattgcc agtgaggcaa aaaactgatt gggaaaaacc
    22381 ctgagtaatg atcgcatgtt gctaaaaatt cgtagcagtg ttatcggtag cggtcgggtt
    22441 tgggtgaata gggaaccaaa tgtagatatt ttctcttaat aagcccaaag gcgtgcatgt
    22501 cggaattggc aacaaagagc aaaagagcta attttaagcc cgtggagaat ggcaacctaa
    22561 cgaaaagcgc gaaagaccgt attttttcga aagagttcct tccgcacatg gacgcgctgt
    22621 ataacttcgc tttccacctt acgctggatg aagatgatgc aaacgacctg gtgcaggaaa
    22681 cttttttgaa ggcgtatcga tttattgcat cgtatcagca aggaacgaat gcgaaagcgt
    22741 ggttgttcaa gatcctgaaa aacgggttca taaacgatta ccgtcgcaaa acgaagcaac
    22801 cctcgaaggt tgattatgat gaagtggtga gttaccacga cacagatgat gaggggcatg
    22861 tggagtattt agatttgcgt caggaattat ttcagggcat gatgggcgat gaggtaacga
    22921 aggccctgag tgaattaccg gtcgacttta aaaccattat cctgctttgc gatatcgaag
    22981 agttctcata tgaagaaatt gcgaggatca tcgatattcc cattggtact gtgcgttcgc
    23041 gtttacatcg ggcacgcaat attttaaaag aaaaactacg cgaatacgcc caaaaggaag
    23101 ggtttaaaga aaaacgttga ttatggacca acaattaccc aaacacgatt gtagaaactt
    23161 cgtaaaaaag gtgttcctag tgcttgacgg agaactgcct gaagaagagc gcaatctctt
    23221 tatcgccgat attcagcgtt gcaaaggctg tcttgaacac tatcatatag aaaagacttt
    23281 taaggatttt ctctcagtaa aagtggaaag aaaagaatgc agcaacaaac tgcgcgatga
    23341 gatccgcaat atggtaaata ataaccccga agcctgatca caatcttctc tttacaataa
    23401 gccaaaaggc aatcaaaatt tttgttggta atttttattg ttggttaaat cgccatgagc
    23461 gacctttaac ctttacaatt actcaccatt gacgaccatt ttcgtccaat taaattaata
    23521 tcctatggaa ccggattata tctctattaa tcgcaaggca tggaacgata aagttgatgc
    23581 tcatgtgcaa tcagaatttt ataatgttgc cggttttcta aatggcgaaa cctccctgaa
    23641 acatattgaa ctggaattac ttggtgatat ttccggaaag cgtattttac acttacaatg
    23701 tcattttggt caggatacaa tttcgctggc gcgcatgggt gcatatgtca ccggcgtcga
    23761 ccttagcgat gtagccattg caaaagctaa agagttggcc gcaacgacag gtgcttcggc
    23821 taattttgtt tgttgcaata tctatgacct cgcgcagcat atttctaccc catttgatat
    23881 tgtttttacc agctatggta cgataggttg gttgccggat ctgaaagcat gggctgcatt
    23941 aattaggcag tttttgaaag cagacggtaa attaattttt gttgaatttc atcctgccgt
    24001 ttggatgttc gacgatgatt tcaaagaaat caaatacaat tatttcaata caggaattat
    24061 tgaagaaacg gaagaaggca cctacgccga tagggatgcc gatatacaca ataagacaat
    24121 aaactggaat cacggcataa gcgaggtggt gcaaagttta ttagataacg atttatctat
    24181 taccggcatt catgagtatg atcattcgcc gtggccttgt tttaagcaca tggaaagcga
    24241 tggtaaagaa tcattttata taaaacatat cgggaagaaa attcccatgg tgtatgccat
    24301 caccgcagag cgaaaagtct gaaatcagta atcagttttt tttccgatag ttattggcag
    24361 gctaatccat gtatcaacag gaacattttg gacttttccc ggaatccagg ccggcatatt
    24421 attcacggct ttaagtacgt tttcgtttgg caataaatta attccctgaa caacatatgc
    24481 atttgcgatg gtgccatttt tttgaacgac aaatcgcaca taaacaacac cttcactcac
    24541 gcttaggggc aattcaagat ttgcattgaa ataatcgact agcgcttttt caccgcccac
    24601 aaattgcggt tgaacatctg ccatcataac agaatcacct tgttggaagt cgtccattgt
    24661 ttctgtatct gatgcgccgg cgggagcccc gctgtaatct tttgttaatt cgttttcttt
    24721 atttggcttg tcttcatctt tacgtttgaa gacttgcttt ttatcactta gcacggcaac
    24781 ctcttgtatt gattttgttt caagaagaaa atctccgttg ctcatttccg gaatagcgct
    24841 taacaccgct acattggtgc ggaaatatgc ctctgcaacg gctggtttta caacatctaa
    24901 attgccaaca gtgctgaaac cttcagacac atttatgctt tcatcaatac tgaatttatc
    24961 atctccggat aaatcttcag ttgcttcaga cgcggtgaat tcgttttcta ttactaccgg
    25021 ttgaacattg ctttttctat ttatttcaat ggtacttgct tctacatttg aatcgcctgc
    25081 gcccatacct atggtatctg cattattaaa tgcgggatgt tgttgtgccg attctacgaa
    25141 ggctatttct gattcacttg tttttttatt gagttcgcgc atgatgatga gggttgcacc
    25201 aaggaacacc aatatggttg ccgcggcagc atactttctc aactgactgc caaaaccggt
    25261 ttttgcacct gcggcgatgc gcgcatctac tttggcgtaa atagcatcca aagctttttg
    25321 cacctgttcg ggcgttgcat attgaagccc ttcgatagca tctgccaaaa atggatcatc
    25381 agccattaat tgttcaatac gacgacattc agcttccgac aattcacctt tgtggtatgc
    25441 cagaagttgt tcggttgtaa ttaaaccgcc tttgatagta tgtaggctcg gtttatccat
    25501 tttgcttcgt caaaaatatc ttcaaatttc gttttccgtt ctgaatgtaa ctttttactt
    25561 gtttcaagtc gtatccggtg atctcagcaa cctcaacata gcttttatcc tgaaggtaga
    25621 acagttcaac acagattttc tgctcctcat tcagggtttt aatgccttcc tccagcctat
    25681 taagctgtgc ttccttgata tcactgtcat catcaagatg cgcggtgagg cctgattcca
    25741 taccaccatc ttcatcattt tcaagattat actccggcaa ttctactgtt tcatggcctt
    25801 ttcgcttcct aatcagcatc aggcattggt ttttggctac catatacagc cagggcttga
    25861 actgctgcac ctggtgctgt ttcagatcga ccatgagctt ctcaaacacc atcatagcaa
    25921 cgtcttgagc ctcctcgggg tctttcatat acttcagacc gacaccgtaa acaaggtgcg
    25981 cataccgttt ataaagctct cccaccaatg aggtgtcgcc cgactgcttg taagcctcaa
    26041 tgagagcctc atcagtcatt tcggaggggc gccgccgacg tggaaaaagg agctgaaaca
    26101 cagggtaaag ataatatatg aggtttaagc gacaatgtat gccacggcta atgaccccgg
    26161 ttaacggttt tccggcaata tggtctcatt cgccggcgtg agcacgacaa gaaagtgcct
    26221 gttggtgaaa agggcttcaa tcaattaatg gatagtttca tgcagaaaat ggagtatttc
    26281 gctcaaaacc gtttgtccgg ttttaggtta gttgctactt tcgaactgta attattcatc
    26341 aacccaatct ttaactatga aaaacacaac ccaaaacaga gcatcacgtc ttattcaata
    26401 ttcagctgct acaattggag caatattaac gcaacatgca gatggacaag tagcctatac
    26461 cgatctggat ccggatgttg atttacatta tataacagga gattcctatt tattagatat
    26521 taacaacgat ggtattgatg attttgagat cagtgcgatt gtttcatcaa ccgtatttac
    26581 tggaagtagt tcttcatcat ttacaagtta tacttataaa gttctggtta ccccgttatt
    26641 ggacaatgaa gttttcttaa ccgatgacgc aaattttatt gaggcaggtg aaacaattga
    26701 tgccgattta acagcaggtg aatttattcc ttcagcaggc gacttcaacg ctgtattttt
    26761 tgaagttcca tcttatatag gtcttaaatt aaatacagcc ggcgctatca actatggttg
    26821 gctaagactt acaaatgata taacaagttt tactttactt gattacgcag tagaattaac
    26881 cgaagatacc ggtatttatg cgcaagtgcc tggtggcccg catgctgtat ccaatatttc
    26941 aacagctgat gtttccgata cccataccgc cgccgatgtg caggtgtcat ttaccattcc
    27001 ggacgatgaa agcggcacat attcatatcg gattttcgcg ggtgaaatta gtttcagcaa
    27061 aatgcaagca attaatgccg atccggaaca atatacagag gtttttccta ccggaagtga
    27121 tcaggcgctt acattgaatc caggacaact ggattatttt cacaacccgg tagaagaatt
    27181 cagtagaata gtagttggtg ttttaagtgt aagtgccgat aatatcaacg aaagtgttta
    27241 tcaatcatat attatacccg ttgtacccgg gcatgatgtg gaacctgtca gcaatgttga
    27301 agtaacacat gtagtcagcc cctatccggg aacatcaatt gaagtgaatt ttactacacc
    27361 tgatgattta cacggtataa ttaattttct gtcgtacata gttccggcag atatgttgcc
    27421 gataattgat tcatctttaa tatatattaa tcccaatcac gctgttctta atgcatggga
    27481 ggttggcgga ggcgaatttg caaccgatca cagctatgaa atgccattta cgggatatga
    27541 tatcaacggc gatgtgctga cctttggcgg gcagtacgga gtatgtatac aatcgcacac
    27601 tcaatggccg gagtatagtg gcttcgcttt tgaatcgcta acatgcagcg atacatttac
    27661 gataacagat gaaacaatag gcatttcaac catgctttcc ggtgaactat cggcaaatgt
    27721 agtgccgtcc ggaatctact tacaatcacc tcaaatgatg gaagcggttg aactattagg
    27781 cataaacgga agtttaattt attcctcatc cattcatgca gacaatacca taataccact
    27841 ccccgaagca tcaggaattt actttataaa agcccgcttt aaagacggct ttggtattaa
    27901 aaagctgaat gtggtgatta aataattggc ctgaattcaa ttatttactg cgctctaatt
    27961 cgagctgcac aaaggcactc atagatggta tatcctggcg taaagtcatt gatgtagccg
    28021 tcagcgccgt gatgttgaat ttttccacgc catgggtggt atccatttcg tagaacatac
    28081 tcaactctct cgtttcggga tcgaattccc accttccgaa agcgccgttt acaaaaaaag
    28141 aactgcgcaa ttcgaatgaa ttatccttgt taagcgtata aacacttgat aaaaattctt
    28201 gttttcccgc ttcctgatat tcggccggaa tattgggaat ggtgctttca aattcttttg
    28261 cattccaaac acctgcaata ttttcttcgg ttaattccgt tttttcgcta catgccgcaa
    28321 acaacatccc gctaataagt atgatcaggt attttttcat tgtgctattt ttataaactg
    28381 tttagttgcc atttcgcctt gctcattatt gcattgcaat agatatgtgc ccataggcaa
    28441 tgcatctaca tttatcacaa tgcgattttc accaatggaa atattcacat cgcttgtaga
    28501 aattatctcg ccattaaggt taaaaatacg aaattgataa ttacctgcgg cagttgcggt
    28561 gaaatttatc atcgcttcat tgacaacggg ttgtgcaatt tgtaaattat tatttaagtc
    28621 ctgttcttcg gaaatggcac tttcatagta atcttcaaaa tatacaattg acgaaatggt
    28681 ttctacgcct aaaactgttt gagtattaat ttgtaaaacc ggtatacctg tttctttact
    28741 taaccagcga tattcattag ttgtatatgc aacaggcaac acgaatgtgt cataagtgaa
    28801 tgtatcaatg atattaatta cagaatgtaa acgcaatgca tcataggttc cggcaggtgt
    28861 agttacttgt ccccatccat caatttcatt gctgcggtct cttgattcat tccatcctcc
    28921 aatgtcggga atgctgatat taaaaccggt atttgaattg ctgatttgtc cataatctgc
    28981 cggaaaattt aatatcactt cgttgtcatc atagccgatt ggaaaaggaa ttcctgctat
    29041 tgtgccagcg aaaccggcaa atgcgaaagt gctgttatcg agttgataga aattatatac
    29101 atctgtgatg gtgatgaaat cattgacaat atttgacacc gattgttcgg cgatatcgct
    29161 agtaaaccac aggaagaagt aaatgggatt ggtatcattt tcatctatcc agttagttgt
    29221 gtcctgggta acggggacaa gtgtgctgaa attccaattg tgatcaggac ccgtatctgt
    29281 gccgtcgaat gcaatcagag ggctggcatt tgttacgagg aaggattcgc cttcgtccgg
    29341 aaggtcactg ctgacaatag tgatgggcga ttgagcctgc atatacgcag atacgccgat
    29401 cgctatcaca aagctgagaa ctgatttcat attacaaaga taacagacct taacaaggga
    29461 atgcgtattt ttcggggatg aagtgggtga cgcctataat atgcttcttt gtaattggtt
    29521 gcagcgcgcc atcggtgaaa caaccgcagt ttaaaggtgg tcagatggta gaggtggtca
    29581 cactgtacga aacagacacc acaggaacca tgtttttatg ggagcggagt gatgaaaaaa
    29641 gaccctggcg ctgcgttgat acgattaatg taagcgttgg cagaaacggc ttagcgcctg
    29701 cagataccac ttacttaaat cacaagcgcg aaggagatgg ttgttcgccc gaaggtattt
    29761 ttccattaac acgtgtattc agttacaatg tgctgaacaa tttatcaatg ccatttgaac
    29821 aagtagacac aaatgacctg tgtgttgatg atggagcttc tgtttattac aatatgttaa
    29881 ttgataggga tactgcacaa ataaatgact ataacagctt cgaaaccatg cacagaaaag
    29941 accggcaata tgcttttggt gtttgggttg attataatac cccggctatt gcaggaaatg
    30001 ggtcatgtat ttttttacat atctggtcgt ctccgggaca ccctacaagc ggctgcaccg
    30061 ctatggcgga atctgatatg ttaaagttga tacactggct cgatgccaaa caatttccaa
    30121 ttttatatca gcatcacctg aagccatatg aaaataatta aacttaccag tattccatat
    30181 ttgtcagacg aaatatttca atgtatatga aaaggttttt atttctcgca atcacatcct
    30241 tttttttgct gccaataaac gcacagaaaa ataacaaaac cactgccgct ccggcaaccg
    30301 aggtaatatc aaaaaattat ttaagcggta tgcattggcg aaatataggt ccatttcgct
    30361 caggaaggtg tatcgcggta accggtgttt ataacaacga tcaaatatat tatgcaggcg
    30421 ctgtaggcgg aggattatgg aaaacatcag acggcggaca tgcatggcat tgcgtgagtg
    30481 acagcatttt cggatctgca agtgttggtg cagttgaagt tgcacaaagc aataccaatg
    30541 tagtatatgt tggtatgggt gaagtggaga tgcgcggcaa tatttcattt ggcgatggca
    30601 tgtataagag tattgatgcc ggaaaaacct ggaaaaaaat cggtcttgaa cagagttatg
    30661 ccatcggcag tattgccatt catccgcaaa atgaaaatat agtttacgtg gccgcacaag
    30721 gacatatttg gggtcccaat ccggatcgcg gattatatcg aagtgtagac ggaggtgcaa
    30781 cttgggagcg cattttgaaa ggaccaaatg ataatacggg ttgcgtagat gttaaattag
    30841 atccaagcaa tccgaacatt atttatgccg gtatgtggaa tgcatatcgc acaccttatt
    30901 cattaagcag cggaggaccg ggaagtggtt tatttaaaag tattgacggt ggaaagacat
    30961 ggaaagaaat tacaaaaaat cccggtatgc caaagggcat gatgggcaaa tgtattgtta
    31021 cgatttcgca tatcgatcat aataaattat gggcttcaat agaaaataaa gataatgccg
    31081 gaatttatgc cagtaatgat ggcggcgata cctggttttt gcttaccaaa gaaaatgacc
    31141 tcattcagcg tccttggtat ttcagtaata tatatgtaga cccgcaaaat gataacgaat
    31201 tacatatttt atctgtggaa tattggcgaa gtattaacgg cggaagcgaa ttcagtcgcg
    31261 cagaaactgc aacctgggac aatcacgtaa tgtggatcaa ccctacaaat gcaaacaatt
    31321 ttattattgg tggcgatggt ggtccaacca ttacatttga tgatggcaat aattggagcg
    31381 atattgatat acctacggga caattttatc atgtcaatct tgataacgac tggccttatg
    31441 gcgtatatgg tgcacaacaa gataattcca gcattaaaat aaaatccaga acaagtggtt
    31501 atagcatcgg agcgtccgac tggcatccgg ttgcgggtgg tgaagccggc tatattgttc
    31561 ccgaccccaa agactcgcgc attacctatg gtggcgaata tgatggtatt ttttcatcct
    31621 ataatgaagc aaatgaagta tatcgcatta tttctgtaga ccccgaacaa cattatggtg
    31681 caggagctat aacggcgcag cgacgattta actggacttt ccctatcgcg ttttcccctc
    31741 acaacaacag ctgtttgtat gccacaagta attttgtgca taggagttat gatgcgggcg
    31801 ttacatggga aacaatttct cccgacctta caagaaacga cccgaaaaca ttacaaacga
    31861 gtggcggcga tattacttta gataatacag gggttgaatt ttacggcacg attttcacgt
    31921 ttgccgaatc gcctgtgaaa gccggtgttt tatggacagg aagcgatgat ggccttgtgc
    31981 atgtatcaac cgacaatggc acaacatgga caaatgttac accaagtaat ctacccgaat
    32041 ggagtatgat cagttatgta gagccttctt attttgatgc cggtacatgt tatttgtctg
    32101 caacaagata caagttagat gacatgcagc catacattta caaaacaaaa gattatggta
    32161 aaacctggga gaaaattgtc aacggattgc cggcaaatat gtacaaccgt tgtgttcgca
    32221 acgacccgaa tgtacaggga attttatatg caggattaga aacaggtgtt tggtattcat
    32281 taaatgatgg cgcattctgg cagccattgc aattaaattt accattggct ccggttaccg
    32341 atattcgcat tgagccaaaa gagcgcgata tcgtaattgc aacgcacgga agaagtttct
    32401 ggattcttga tgacataacg ccattatatg atcttgcgaa aaatggtgat gctattcaaa
    32461 aaagcaatgc atatctatat cctgtaaaca caggttatcg cactccgggc ccatcctatt
    32521 atgcagagga agcgagcgat cagaccggag agaatgaagc agtgggcgtt aaaattcgat
    32581 attatctaaa acaaaaaagc gaaaaagaat tgcgtttggc gttttatgaa agcgatggcg
    32641 atttaattat tacttatagc aatttatatg ataaaaaacg cacgccgata accatcaaag
    32701 atgaatttta tccaaatccg aaagttacgg aatcaggaaa attaaatgca tttcccggta
    32761 gtcaccagtt tgtgtgggac atgcgatatc cggatgcaac aggtgatacc agtgcaacat
    32821 ttgaagcctc tttgatggga ccacttgccg tgccgggaaa ttatatggtg agaatgtaca
    32881 ttggcgattc actggtaggt cagcaaacat ttgtaataga ccgtgatcca agaaacccgg
    32941 caaccgttgc agacttacag gcacaattcg atatgaacca acgcatttgt aataaactca
    33001 atcagattgc agatgcaacc ggttccattc gagaaacgcg tgatatgatc aatgaacata
    33061 ttggcgaaga cgcttcggaa gaaattaaat caaaaggaaa acaactgatt gatgctttga
    33121 ctgttattga aggagaatta tataatccta aaattctggc atacgaagac aatttaaaat
    33181 ttcctatcat gctggaagaa aaacttgcgg gattaaatta cttcttgcag atggcagata
    33241 ccgccccaac aaaaagcatg tatgccaaat acgaggattt aaatgcgcgc atcgatgtgc
    33301 aactcaatgc actgaataaa ttgatgagca ctgaagtaaa acagttcaca caaatggttg
    33361 gagaataaga catctaaggc caatttgtat tcatttttgg gccttataag gcttctttta
    33421 cggttccttt tgatggattt gcagaagaag tgtcgccagt caaaaccctt atttagcagc
    33481 attttgagcc gattttaatg tctatacaga cttcaaaacc tgtcggcacc ttggaaatga
    33541 gtcatttttg tatttgaagg gggtgtaatg tcgttgcatt tttgtttcat caaaacaaca
    33601 acgccatgtt acatcctgaa attctcagac aaattttacc cggattagtt acttcagttg
    33661 ctatacgatt tccggttgcc tatatccgca gcattacatt tcatgtacat gaaaaaatgg
    33721 tagctgcctg tttctggcaa caagaggagc aacgcgctgc gccatttaca ataggagaaa
    33781 ataatatgcg ttcgggatgt tacatctaca actatgatga aaacattcgt gaaagcgaag
    33841 tagaaataac agggatggat gaaagtggta aagcgttttt aatttctatg ccttataatc
    33901 gcatagtacc aatgatagca gcctgatcag cgatcttcgg ggggattttt ttcaacccac
    33961 attttattaa acgatttcgg ggcaaacttg aacattgcgc ggcgctttcc ccaagagttt
    34021 ttgaaaagca tattcacacc catatttttt attgccggcg atggcttatc catcgatttt
    34081 ctgttcaaca tagaacgacg ccacattttc caggccattt tctcgctgaa agtaccatga
    34141 ccttcttcca ctgcttcatt tctgttatac agtaataaag tgtggagatc gatatgaaca
    34201 ggacatactt ctgtgcaatt accacataga gaacttgcat tgctcaggtg tctgaattct
    34261 tccataccct tgaaatgcgg ggtaataata gaacctattg gtcccgaata agtagcacca
    34321 tatgtatgcc cgccgatgtt tttataaaca gggcaagcat tcaaacaaga tccacagcga
    34381 atgcaataca atgcttcacg ttgcttttct tcggcaagta atttacttcg tccgttatcg
    34441 agtaaaataa catacatttc ttccggcccg tcagcttcaa cttctttttt gggtccggtg
    34501 aaaatggtat tatacactgt tactttttgc cctgtaccat aagttgccaa tagcggccac
    34561 aataacgcaa ggtcattaac gcctgtcagc attttttcaa tgcccacaac tacgatatgt
    34621 gttttcggaa agctggtgct gagtcggcca ttgccttcat tttcggtaac agctataccc
    34681 ccgatatcag gaagaataaa attggcgcca ctaaaaccca catcggcctt cacatatttt
    34741 tcgcgcaaat ttctgcgggc aaccaatgtt aattcctgag cagttaggtc gaggctggtg
    34801 ccaagttttt cattaaataa tttcgctaca tcatctttgc ttttgtgcat tgccggcgta
    34861 acgatatgat agggcgcttc cccatctaat tgctgaatat attctccaag atctgtttct
    34921 acgctttcaa ttccatgttc ggccaggaaa ctgttaagat gaatctcttc ggttgccatg
    34981 ctttttgcct tcaccgccat gcgcgcttct tttttcttca ggattttgag aatttcctgt
    35041 tgcgcttctt tttcatcgtt ggcccagatt actttacctc cgtttgcggt gaaatttgat
    35101 tcaaactcga taagatattt atcgagattc tccatcacct tccacttgag gttttttact
    35161 tttttccgca catgctccag atcggcatat tgctttttac cctgaacaac agtagcattg
    35221 tacttgccga tattgaacaa caattttctt ttgtgttcag catcaaaagc tttttgttct
    35281 gctttttgta aaaatgtatt cagattgcct gacatgctgc cggtggttgt cacaaaaata
    35341 acttatctga ccataacttt ctttgacgga ttgtgtaaaa acatcctctg tgtgtatttc
    35401 tgcatacaat tcactcattt atacctttag caccttgctt gatagccact attttcaata
    35461 ccttcacctc tatgaagcag aaaatacttc ttcttagcgt gcttttggcg atcgttcctt
    35521 taagcacata tgccggcaca acttccaatg gtcccacatt tgccggagtg ccgtatgagt
    35581 tcattctatt cggattgaca ctgctcggaa tcgccctctt tcataaatac acgctaaaaa
    35641 tcgctctcag tgggcttgcg gcggttcttg tctataaaat tttcgtcc
//
LOCUS       DAOQOU010000011        34568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_525_length_34568_cov_6.036160, whole genome shotgun sequence.
ACCESSION   DAOQOU010000011 DAOQOU010000000
VERSION     DAOQOU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 34568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34568
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_525_length_34568_cov_6.036160"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLM90_01860"
     CDS             <1..642
                     /locus_tag="PLM90_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ88140.1"
                     /translation="QDDGKIIAAGYTYDINGDESIAIARYASCSAYFTLFPDATPHVW
                     KAVNMAVGSDPMSYVWEWGDGTSSTEPYPSHTYADSGYYDICLTITDGLGCTSTYCDS
                     STYIYRMSDELAMVTINVWEELPVETETITADFSVTPNPFSDQITIEIPSAVSSSPNA
                     EISIFDLAGNILLTQIISAPQTTINTSQLPTGIYVLSYHNGLEFGTAKLVKME"
     gene            complement(748..873)
                     /locus_tag="PLM90_01865"
     CDS             complement(748..873)
                     /locus_tag="PLM90_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88141.1"
                     /translation="MSVFVYALKNNINNEIHIGNAIGEEITLIEYKQGLSILTIF"
     gene            1061..1411
                     /locus_tag="PLM90_01870"
     CDS             1061..1411
                     /locus_tag="PLM90_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88142.1"
                     /translation="MSLFDNIKKEEVEIIATVKKIEIVYTYKGDIQKITLICDSEKLK
                     KGYETYFNYSTKWSKFNLKSGDKLKFKAILKTTTVTVHKDERWVDDWGTLPIHLTDMV
                     DFIEIQSPKQIMLF"
     gene            complement(1555..3237)
                     /locus_tag="PLM90_01875"
     CDS             complement(1555..3237)
                     /locus_tag="PLM90_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88143.1"
                     /translation="MKPVSILFILICSCIYLQAQVTSVGLAAPTAVFDVSGSPVTTTG
                     TLTLTFDSQSQNLFFASPDGMSGTPSFRSILATDLPIVGSGTQGIISNNTGSTQVFGD
                     GDKEFPDKLFLSNGTTANPSLAFSGAHTTGLSWVTGEAINGSFSGTQRFQFTSLGKLV
                     LSDCEEFDPKLDFNIYHDSNDDSPAQDGGINITTYGGTDCVYPDCHFATVTGRTASGS
                     KGTPQATVVDQGLIELSGKGYTGSAFETSNRGVFGIYAAETHDGSGQGTYALVRTTPI
                     NSTTIHTSMLIDPEGNAGLTHSSFNTSLPNGWDATADSRFFSIESNATNKDAGILMQN
                     ASGNRGLNVWFDYSGNFAYIDDIRNDQASGVGFRFRTAGTPEAALTMLYNDAGDHVYI
                     NAGKDVTPDNLFTVGDNSEFQVDVNGKVAKYNNIATVSNGVPAEYAIAQATNKSAAQA
                     ATTIYTTPAADGFYRISWVCTITTQASTSSILGPFTVSYTGATDNVVKTWPSNNINNF
                     NQCATNSTATGVISGCMTIYAKASTNIQFTVGYTSSGVTSMVYDYNIVCEKL"
     gene            complement(3267..>4346)
                     /locus_tag="PLM90_01880"
     CDS             complement(3267..>4346)
                     /locus_tag="PLM90_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ88144.1"
                     /translation="ESFGDLDYWIVALDTLGNVEWQNDFGGNNSDYLKGIIQTFDKGF
                     ALVGYSLSGSTGNKVEGNYGMEDYWIIKVDTLGNISWQNSLQGTGSDFACCIIQAPDG
                     GFLVGGTSDSNISPDKNEDNIGNGDYWIVKFYPEDCTLYTFYKDFDEDGYGSMGDSVV
                     ACYIPDGFVENNFDCNDFNPMIHPDQGDSCNDIDDNCNGILDEDATFTTYFLDYDEDG
                     YGNPLIDTISCSTIMGFVLNDDDCDDSNPLVYPGAIELENGVDDNCNGSVDEGFNYIE
                     NIDTSIYKIFPNPNTGEFKIYVNSSIIELLVIKIYSITGELLYYSTFLSSNEIKMSLP
                     EAFSGLYLLSVVSDKVYIFPIEIIK"
     assembly_gap    4347..4446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4447..5431)
                     /locus_tag="PLM90_01885"
     CDS             complement(<4447..5431)
                     /locus_tag="PLM90_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88145.1"
                     /translation="MKLILFIVLAFFVEFAYSQAPEIEWQNTIGGGSSDNLSCISLCH
                     DGGYIIGGSSGSTIFGDKSEDNIGYLDYWVVKLDSVGNILWQNTIGGSLTDILTSVIE
                     TNDKGFLVGGYSESGISGDKSELNFGSDDYWVVKLDSVGNILWQNTIGGSSSDQLTNI
                     IQTRDDGFLIGGRSSSNISGDKTENAIGGTDYWVVKLDSVGNILWQNTIGGLNFEELY
                     ELMQTEKGDYLIGGNSNSGISGDKTEASILNPFGLNTHDYWIVEIDSIGNIIWQNTIG
                     GNDADYNYTLDLISNDGLITGGSSKSNISADKTSESFGDLDYWIVALDTLGN"
     gene            complement(5459..7345)
                     /locus_tag="PLM90_01890"
     CDS             complement(5459..7345)
                     /locus_tag="PLM90_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88146.1"
                     /translation="MAEKFKILFINISLCASCTFAQSPSIDWQNTIGGSSDDVLNSIQ
                     QTSDGGYILGGSSKSIISGDKTETHAGQDYWVIKLNSAGFIEWQNTIGGSSDDYLNCI
                     QQTSDGGYILAGYSNSLISGDKTENSIGGFDYWVVKLNSTGGIVWQNTIGGTLDDYLH
                     SVQQTSDGGYILGGESKSDITGDKTENNFGLTDYWVVKLNSAGNIVWQNDIGSWSDEY
                     LNTIKQTSDGGYIIGGYSNSGIAGDKSEACLGGNDYWVIKLNSLGVIEWQNTIGGNSE
                     DNLFSAEQTLDGGYILGGWSLSGISADKTDAGIGNYDYWVVKLNSTGGIVWQNGIGGT
                     DADYLYSIQQISDGGYFLGGYSRSVISGDKTEAKIGGNDYWILKLNSTGTIIWQNVIG
                     GSSGDLIQSPNTIDETSDGGFIVGGYSSSGISGDKTEVSLGSTDYWVIKLMGDSVLIC
                     GVPTGLFSDNITSTSAKVHWSIIPTADSYKVYYRISGTGPWTKKTAVSNNKNLPGLTP
                     FTTYEWKVKSNCGPESSDFSEIQTFITLPMKEGNLINNQLKLIRVYPNPTSGNIVISF
                     ENTITRTRAVSITDISGKYYLQTNLLFENGLSKFDISKYPSGIYILKIITDGNEFIQK
                     VVKE"
     gene            complement(8361..8435)
                     /locus_tag="PLM90_01895"
     tRNA            complement(8361..8435)
                     /locus_tag="PLM90_01895"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8400..8402),aa:Glu,seq:ttc)
     gene            8488..9540
                     /gene="gap"
                     /locus_tag="PLM90_01900"
     CDS             8488..9540
                     /gene="gap"
                     /locus_tag="PLM90_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMZ88147.1"
                     /translation="MSPKILFRQPTFAPIMSTNYRVAINGFGRIGRIFFRRALLKANF
                     EIVAINDLTDVDTLAHLLKYDSVHGRFQGTVSVENGNLVVNGKTIRVSAMKDPAQCPW
                     KELNIDLVIESTGLFLSREKAELHIQAGAKKVLLSAPPKGDDIKAMVLGVNDEILSPA
                     DKIISNASCTTNCATPMIKVIHEHFHIESAYITTVHAFTSDQRLHDAPHKDLRRARAA
                     TLNMVPTTTGAAKAVTLAFPELKGRVGGAGIRVPVADGSITDITCTVTKPTTIEEVNA
                     KFKEASETYLKGILSYETDPIVSVDIVGDPHSCIFDAELTSVIGNMVKVLGWYDNEYG
                     YSSRLADLTERLATMA"
     gene            9801..10997
                     /locus_tag="PLM90_01905"
     CDS             9801..10997
                     /locus_tag="PLM90_01905"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMZ88148.1"
                     /translation="MRTIQDFNFAGKRAVIRVDFNVPLDKATFAVTDDTRLRAALPTI
                     NKILHDGGSVVLMSHLGRPKGGPEDKYSLRHIVAHLSQLLNRDVQFVNDCIGDMVMSA
                     ARQLQPGQVLLLENLRFHPEEEKGDHAFAEQLSKLGDVYVNDAFGTAHRAHASTTIIA
                     NYFNTGNKMFGALMAAEVSAGNKVLHQHEKPFTAIIGGAKVSDKILILEKLLEKADNV
                     IIGGGMAYTFYKAMGGQIGNSLCEEERLDTAKQLLETAKAKGVNILLPVDSITADKFD
                     NDANIGSANSDQIPDGWMGLDIGEKAIADFTAAILKSRTILWNGPMGVFEMSNFSKGT
                     IAIARAIAEATANGAYSLVGGGDSVAAVNKFNLADKVSYVSTGGGAMLEFFEGKTLPG
                     VAAIAD"
     gene            11113..>11296
                     /locus_tag="PLM90_01910"
     CDS             11113..>11296
                     /locus_tag="PLM90_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88149.1"
                     /translation="MPEHWHHIPFLRFLLPLVCGVLIALQFHECYWWIYGLFFVLFIA
                     YLLIGIVTGWTVKFRSQ"
     assembly_gap    11297..11396
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11397..13061
                     /locus_tag="PLM90_01915"
     CDS             <11397..13061
                     /locus_tag="PLM90_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HMZ88150.1"
                     /translation="PVYGDYALIENCFKSPDPPKNPNTFDFPKYLRGIGIAHIARVDA
                     SQLVFTGERHQKLWWTFIFRAREMFHDAVMREVKNGDAQSVGEALVIGTKTAIDPEVQ
                     QAYANTGTMHVLAVSGLHVGILFAVLEFLLNPISWFKRNKYGRVSKFLFIITIIWWYA
                     CLTGLSASVNRSAVMFTCLATGKLFSRQNNSFNVLFVSMFLLLVDDARCITQVGFQLS
                     YLAVGGILFFQPYLQKLWRPKWKILNHIWTMTTVSVAAQISTSPISLFYFHQFPNYFL
                     LSNMLAIPISFVVLVLGLIFFVVNGIPFLGPATGMALDYSLRLLNGSVMAVNQMPGAV
                     INNIYINEWQLGILYVFVLLLGIALLSKQTMYLFRAAFCLCIVWMLAVFRDVKAREDR
                     RLVAYSIRDHDCMLLQGAGDAILLSDTLIDQNSSIWTLNIRNDALKSGVMPDFALFSL
                     QEPFNGASNVLYGYPWLIGGGKVVFLLDPTSVHQLPKSAPEADWVYILGNPFINLPDL
                     QSRFPNATFVIGGSTSYKRHSYYLNTCKKLQIPVHSLRFDGALVLE"
     gene            13311..13766
                     /gene="mraZ"
                     /locus_tag="PLM90_01920"
     CDS             13311..13766
                     /gene="mraZ"
                     /locus_tag="PLM90_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HMZ88151.1"
                     /translation="MAFLIGEYECTLDVKQRIRVPAGLLGQLTGDDAGKFVITRGIDP
                     CLYLYPMSEFHIIMDKVNQIPEYSEEDREYKHMFFSGTSILQIDSADRILLPKLHLAH
                     AGIVKEMVMTCEKNKVVVWSMERFREKFLNRSSLDYQQLAEKVLGKYGL"
     gene            13763..14686
                     /gene="rsmH"
                     /locus_tag="PLM90_01925"
     CDS             13763..14686
                     /gene="rsmH"
                     /locus_tag="PLM90_01925"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMZ88152.1"
                     /translation="MSEAYHIPVLLHPSIQALITDPDGIYVDATFGGGGHSKAIVEQL
                     SPKGKLFCFDQDPDAKKNFIDAFSATNATFIPQNFRHMQKFLRVNGVSKVHGILADLG
                     VSSHQFDTGDRGFSIRFDGPLDMRMAQQGTQSAYALINESSAEQLQYIFGHYGEVRNA
                     RQLANAIVQTRNLFAATGGIRTTTQLRDVALTVLKGEKNPYLAQVFQALRIAVNDEMR
                     ALEEMLESAASLLMPQGRLVVISYHSLEDRLVKNYMRAGNAAGEAEEDIMGRKNVPFK
                     VITRKPVVADDEELSINPRARSAKMRVAEKI"
     gene            14701..15069
                     /locus_tag="PLM90_01930"
     CDS             14701..15069
                     /locus_tag="PLM90_01930"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HMZ88153.1"
                     /translation="MAQQEKNNKGKEQKRSLASMLKSFDEVAFFKWENILANIPYFLF
                     LVVIGIFYIWNNHRGVAMDRDIRDLNTHLLEKQYFYNATSDSLTQQSRQSTVARMVDT
                     LQMQELSHPPYTIKTNDGKH"
     gene            15056..17143
                     /locus_tag="PLM90_01935"
     CDS             15056..17143
                     /locus_tag="PLM90_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HMZ88154.1"
                     /translation="MENTKKDILWRLYLTFAGIVLLCLAIIAQIVNVSFVHGETYRAM
                     ADSVRTKQVSIPPARGSIYSDKHEVLATSFPYFNIYMDPCAASEVDFRRDVDSLSIAL
                     ARLFKDKPASAYKQKITTARKQGKKYLEIHKRVTLPQKQLMQTFPLFRIGQNGGGFIV
                     EPYEKREYPYGSLAVRTIGYVRNGRGIGLEGAYDTLLSGKPGMAVMRKVAGGSWIRLS
                     EQNEIDPVDGVDIITTLDINMQDITETALRNALVKNNAKWGTAVVMEVKTGKIKAISN
                     LSRESEGVYHEDYNYAILEKVEPGSTWKLFSLMCLFEDGLSINDHVDLNFGQYPFADR
                     IMYDSEPHGRRDVTVKTAFALSSNVGISRLAWDMYQKDPESYVKHLQESGIVNVTGVE
                     LPGEPKPYFKQDPSDKNHWYKTTVPWMSVGYELLITPLQLATFYNAVANDGVMMKPYL
                     VEETQQHGKTLQKFEPVVLNEKLCSSKTIDQLHQCLLAVVDSGTAKHLKNDYYKFAGK
                     TGTAQLLENGVYGHNYLSSFAGYFPYDNPKYTIVIIINSPSNGPFYGGAVAAPAFREI
                     ADKVYSHFVNMREPVNAPSAANTGVQAFSKGYAYDYRQIFKTLKVNEDFNDNEEWIAV
                     SANGNNSEHTELKTYQYTMPDVRGMGLRDAMYLLDTYGLNVAVSGAGKVTIQSIPPGT
                     AIQKDNYVMLTLE"
     gene            17140..18609
                     /locus_tag="PLM90_01940"
     CDS             17140..18609
                     /locus_tag="PLM90_01940"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMZ88155.1"
                     /translation="MKSLKDISSQVRILSQHGVSLTSVQIIGITADSRRAAEGMLFVA
                     VKGTTVDGYDFIPTAIAQGAVAIVAERKIEVPDNVCLIIVDNAAAALGIMAHAFYDYP
                     STKLKLVGITGTNGKTTTATLCYHVMRDLGFNTGLISTVENKINEEVIPSTHTTPDPV
                     QLNALLAKMVDAGCAYAFMEVSSHAAHQHRIAGLQFVGGVFTNITHDHLDYHKTFKDY
                     IDAKKMFFDGLTSDAFALVNSDDRNGKIMLQNCKAKHATFSVLGTGDFTARIKENVIS
                     GLVLQLDGVEFHSLLLGEFNAWNILTVYAIFRLLGFEKQQVLTVLSAQKPVEGRFDVL
                     YTGDRSITGVIDYAHTPDAVEKLLSTVRSMLKKDQHLLTVVGCGGDRDRGKRPVMAAT
                     AAKISDKTILTSDNPRSEEPDAIIKEMESGLNEETRKHSLSITDRKEAIRTAVMLAKK
                     GDVICVVGKGHEKYQEIKGVKYPFDDKEVLRETFKTLAH"
     gene            18609..19913
                     /gene="mraY"
                     /locus_tag="PLM90_01945"
     CDS             18609..19913
                     /gene="mraY"
                     /locus_tag="PLM90_01945"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMZ88156.1"
                     /translation="MLYYLFNWLDQQYDVPGAGMFQYLSFRAGLAVLLSLIFSIVIGK
                     RLIAYLQKKQIGESVRDLGLEGQLSKKGTPTMGGIIIILSLLIPVLLLADLKNIYVIL
                     MLIVTVWMGLIGFLDDYIKVFRKNKEGLRAKFKLMGQVGLGAIVGLVLYFSDDIKLRD
                     YDMVYVYAVENGQPVGRPIDAYPANDTRLESDGILNSDSTHFLSHDTRSTKSTIPLVK
                     NNEFDYEWLVSWASPETQKWLFPIVFVAIIIIIITAVSNGANLTDGLDGLAAGTSTII
                     VLTLLIFAYIAGNSILSDYLNIMYIPDSGELVVFSAALIGACIGFLWYNAFPAQVFMG
                     DTGSLTLGGIIASLAIVIRKELLIPVLCGIFLVENLSVMIQVSYFKYTRKKYGEGRRI
                     FLMSPLHHHYQKKGMFETKIVTRFWIITILLAIVSIVTLKVR"
     gene            19913..21265
                     /gene="murD"
                     /locus_tag="PLM90_01950"
     CDS             19913..21265
                     /gene="murD"
                     /locus_tag="PLM90_01950"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMZ88157.1"
                     /translation="MAKKLVILGSGESGTGAAILAQQQGFEVFVSDKGKIRDSYKHEL
                     SSRGIAFEEGNHSMDVILAADEIIKSPGIQEKYDVMKQIRAANKIVVGEIEFAWRYCK
                     GKVVAITGSNGKSTCTSLTYHMMKKAGCDVAIGGNIGKSFAELVAHTNHEYYVLEVSN
                     FQLDDAVTFKPMISVLLNITPDHLDSYNYEFQKYIDSKFRITMNQTDGDHFIYCADDP
                     VTSEEMLKRHSPFLSNPANLLTFTLDDRQHEGAWVADNSIHIQINKNHFTMTINELAL
                     QGKHNQYNSMAAGIVGKVLGLKNESIRESFTDFSGLEHRLEFVAKVHGISFINDSKAT
                     NINSTWYALESQADPVIWIVGGIDKGNDYSLIADLVKKKVKAIVCLGKDNTRIHEAFG
                     KMVDILVNTETMEDCVQMAYRLGKNGDIVLLSPACASFDLFENFEDRGNQFKKQVRLL
                     "
     gene            21305..22516
                     /locus_tag="PLM90_01955"
     CDS             21305..22516
                     /locus_tag="PLM90_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HMZ88158.1"
                     /translation="MSNSIQYLFSRVRGDRYIWLVVFVLSVFSILAVYSSTETLAYRS
                     AGGNTEYYLVKHSVILLFGIVLMYLSHLIPYKYYSRIAQILLWLSVPALLYTMFLAPE
                     VNDASRWITLPVINLTFQTSDLARFALIMYTARVLSKKQDKVDSFKEAFIPVILPIAI
                     ICALILPENLSTASVLFFTCIVLMFVGRVKFKYILLTIGAGIVLIGLIIALAYAFPHV
                     GRMGTWKARVDSFVDGGTSDDTYQVDQAKIAIAKGGVFGVGPGNSTQKDFLPYPYADF
                     IFATILEEYGLMGGIFVIFLYLVFLYRCIKIVVKAPTSFGALLAVGLGLCLCIQAFIN
                     MGVAVHLLPTTGLTLPLVSMGGTSLWFTSLSIGIILSVSREIEKADEVEEEDEQTETE
                     EQSEEEPELAK"
     gene            22534..23637
                     /gene="murG"
                     /locus_tag="PLM90_01960"
     CDS             22534..23637
                     /gene="murG"
                     /locus_tag="PLM90_01960"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HMZ88159.1"
                     /translation="MASSINILISGGGTGGHIFPAIAIANEIKRRRPDANILFVGAQD
                     KMEMEKVPKSGYPIEGLWISGFHRGLDWRNLMFPIKLISSYFKSGSIVKKFEPDIAIG
                     TGGFASGPALNAALRHQVPIIIQEQNSYPGVTNKILSKKAKAVCVAYDGMEQFFPAGK
                     IVKTGNPVRQNIVLCNVKPEKARADFDIDIDSKVLFVVGGSLGSRTLNNCMIGGLQEL
                     ADAGIQVIWQTGTAMYEMCKHAAKGYDNVRVLDFIANIDHAYAAADIIVSRAGAIAIS
                     ELCLVGKPVILVPFPAAAEDHQTKNAMALVKENAAMHIADKSAPVELIPNAIQLMQDH
                     DRRSLMAQRIKNFAIADAAERIVDEVFKYIQAK"
     gene            23641..24987
                     /gene="murC"
                     /locus_tag="PLM90_01965"
     CDS             23641..24987
                     /gene="murC"
                     /locus_tag="PLM90_01965"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HMZ88160.1"
                     /translation="MDIAQINRVYFIGIGGIGMSALARYFMRQKCVVSGYDRTETEIT
                     DALQHEGAQIHFSEDLTQIDTQADLVIYTPAVPPTHAELIYYRDNGFTVLKRSQVLGL
                     ITKDKFVIAVAGSHGKTTVSSMIAWILKNSGYDCTAILGGISTNFNSNYVMGFNDVFV
                     VEADEYDRSFLQLSPNIAVITATDSDHLEVYGTQEAIEATFLEFANRIKENGLLISKP
                     RLGVFQKYQRDKRGYDLTDSKADLYAVTYAYEGDRCHVTLNDGYEFYLGYPGVHNIEN
                     AIAAISVALHLNIHPDLISRAITAFTGVHRRFELVNGVFGKIPYYDDYAHHPEEIKMF
                     LTSIKQLFPNKKITAVFQPHLFTRTRDLADGFATSLDIADEVILLPIYPAREEPIPGV
                     TANNILDRMKNPHRRILEKADLIKYVSKADIEVFCTIGAGDIDKLVQPIAEILKGR"
     gene            25005..25781
                     /locus_tag="PLM90_01970"
     CDS             25005..25781
                     /locus_tag="PLM90_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88161.1"
                     /translation="MRVFGILLLVACGTGLLLLTAFNADRQDASACIDVQIHVDEERG
                     IYFLDANDIRTILATEYGDSVKGDALGSIRVGRIETLLERNPYVDDAETWVDATGKLH
                     IEIVQKQPIARVINKYGVNYYINNIGNKIPISDKFTARVPVVTGSIEEGIHNADMATT
                     SALQNILKITRFINADPFWSAQIEQIAVANDGTFTLIPKLGDHKILFGGIAHMEEKFR
                     KMQVFYTEGLNHTGWNTYSAFQLDFEGQIVCEKNKNYEQQ"
     gene            25768..27174
                     /gene="ftsA"
                     /locus_tag="PLM90_01975"
     CDS             25768..27174
                     /gene="ftsA"
                     /locus_tag="PLM90_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMZ88162.1"
                     /translation="MNNSNRIITGLDIGTTKICAIVGKITDHGKIEILGMGKADSFGV
                     MRGVVANIDKTVEAIRRAVAEAQEKSGVEINEVYVGIAGQHIKSLQHRDILTRNDAES
                     EIGMDDIDRMIENMHRLVLPPGDRIIHVLPQEFIVDGEQGIKDPIGMSGVRLEANFHI
                     ITGQISAAKNIFKCVEKAGLKVIDLILEPLASSASVLSEEEKEAGVALVDIGGGTTDI
                     AIFQDGIIRHTAVIPLGGNIITEDIKEGCMIMRNQAEILKVKFGSALATETEENEIIS
                     ISGLKGREPKEIKVENLARIIQARVEEILEHVYYEIKLSGFHKKLIGGIVITGGGAQL
                     KHLVQLVSYITGMDARVGYPTEYLQKAKAEDIRHPMYATAVGLILKGHDDRNYREREI
                     EMPAPQQQQEIVNPVVDKMPTSNEQLNQVAEKEMNDATMEMEQELAHDKHAKPSWLKN
                     MLSSTRKWFEEDDVSDFK"
     gene            27238..29148
                     /gene="ftsZ"
                     /locus_tag="PLM90_01980"
     CDS             27238..29148
                     /gene="ftsZ"
                     /locus_tag="PLM90_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMZ88163.1"
                     /translation="MYFDIPKEQASIIKVIGVGGGGSNAVNHMFLQGIKDVNFVICNT
                     DQQALEMSPVPNKVQLGPSLTKGRGAGSHPTVGQQATMESLQEIKSILEKNTEMVFIT
                     AGMGGGTGTGGAPVIAKLAKDMGILTIGIVTVPFGFEGKRRKSQAFEGLETLKHYVDA
                     ILVISNDKLREMFGNLAWNEAFSQADDVLATAAKGIAEIITVPGYVNVDFEDVKTVLR
                     GSGLAIMGSGKAAGQDRALEAVRVALESPLLNDNDIRGARSILLNITSGTTPVLMDEI
                     AEITEFVQEAAGNDCDIIWGNCNDESLGESIMVTLIATGFETVEQRNQRIKQTTVKVT
                     HVNTHELLQKKVTPIEEPIAEVKEPEAEPEAVITLKHEPKEDKKAKKEEVKETKEVKE
                     QNPSQFSFDFGLFANTIQEKPVINEVPPVVEPPVAKIEEVSFEEKEELVIELKKQDPI
                     TEIISEQEEETAEEEVMSPVEMHLRPSVIIEPVNEFEEPVAEEEALDMPMMNEVEEET
                     IFEIKTNIRHEAIEPEAEIEEEQIMIRKEEQRVSQKQEGRSFITNDDKINVVNSTGND
                     RINRLKSMSMKLSGNTLDEVEKVPAFMRRNIELEDTPNAREINVSKYNIVEGEDGPEL
                     KKNNSFLHDNVD"
     gene            complement(29207..29740)
                     /locus_tag="PLM90_01985"
     CDS             complement(29207..29740)
                     /locus_tag="PLM90_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ88164.1"
                     /translation="MIEHQHSNCFFITFNCRNHFHLLKSDVAKQMVIDSLLRYSEIYQ
                     VSLLGYVIMPNHVHMIILFSGRVRLSDFMRDIKKYSSVLIRDFLLLNHPGETFKINFI
                     QREQLHKIWQDGYHRKVVFTAGFLKQKLNYIHRNPLQEHWKLVEAPELYKYSSANYYY
                     ANKQESEIVTHFGDFFR"
     gene            complement(29983..30843)
                     /locus_tag="PLM90_01990"
     CDS             complement(29983..30843)
                     /locus_tag="PLM90_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ88165.1"
                     /translation="MTPEWLNSNQYPFAPKYLSLPNAQMHYIDEGSGEVILFVHGTPS
                     WSYEFRDAIRILSRNYRCIAMDHIGFGLSDKPADYSYTLDKHKENLMSLIAHLQLSQF
                     NLLVHDFGGPIGLSVATSMPEKINKIIMANTWCFSVADEPEFKRMKAILGSPLLPFLY
                     RNFNFSARYIIPAAFGERTRLTPEIHAQYMKPFAKRSSRNGTIGFAKSLLKDQAWFEN
                     VYRQIGVLVNKPALLLWGLKDNFITEKYLHRMQEIFPNAEVVQYADAGHFVFEEKGSV
                     AAVAIERFLA"
     gene            complement(30840..31379)
                     /locus_tag="PLM90_01995"
     CDS             complement(30840..31379)
                     /locus_tag="PLM90_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ88166.1"
                     /translation="MNQKAHITKTALSLFAAQGYGNTSVAQIAKQAGVSQGLMYNFFS
                     SKDALLEAILAEGMSDIANSILEYQNGAAPKDALRAHIKNTFRIVSENNAYWRLFHSI
                     RMQEPVRTLLGERYAAIQKEILHTLTATFRKLSYEHPKQEARIFFALIDGLVGHYLLD
                     PSQFQLSLIQKTLFKKYQL"
     gene            31474..32502
                     /locus_tag="PLM90_02000"
     CDS             31474..32502
                     /locus_tag="PLM90_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HMZ88167.1"
                     /translation="MAVQVYSADDIISRTKRRHGETKIGERIQTITNADKWQDDLATS
                     TASFVVMGIPEDIGVRANHGRGGAYAAWKPSLDFLINMQSNHFLDGSEMLVLGHVIVD
                     DLMQQADKLDYKREEDIQTARRLVEQVDNRVEHIIHSIVAAGKIPIVVGGGHNNAYPL
                     IKGTSKACGNPIHVINCDPHSDMRPLQGRHSGNGFHYAWHEQLMSKYAVFGLHESYNI
                     YSALELFAQNPEYLTFETYDDIFVRESVNFFDALKRCMDFVDGAPCGIEIDLDSIQNV
                     PVSARTPSGMLPVDVRKFVFHCGENLPAKYLHIAEGAPVLAHKQVDNKTGKLVAYLIT
                     DFVKGKVN"
     gene            complement(32783..34171)
                     /gene="lpdA"
                     /locus_tag="PLM90_02005"
     CDS             complement(32783..34171)
                     /gene="lpdA"
                     /locus_tag="PLM90_02005"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMZ88168.1"
                     /translation="MNYDVIILGSGPGGYVTAIRASQLGLKTAIVERAELGGICLNWG
                     CIPTKALLKSAQVFDYMKHAADYGITVSDAKADFGAMVKRSRDVAGGMSKGVQFLMKK
                     NKIEVIEGNGVVKAGKSVEVTKADGTKSTITAKHIIIATGARSKDLPTMKQDGKKIIG
                     YREAMTLPLQPKKMVIVGAGAIGVEFAYFYHSIGTEVTIVEFMDRLVPVEDEEVSKEL
                     ARQFKKNGINIMTSSAVESVDTSGAGCKVQVKTAKGVETLECDIVLSAAGVVANIENI
                     GLEQVGIKTEKGKIVVDKYYRTNIEGYYAIGDVTPGPALAHVASAEGITCVEAIAGHH
                     PEAIDYGNIPGCTYCFPEIASVGMTEAAAKAAGYELKIGKFPFTASGKAKAGGTPEGF
                     VKVIFDAKYGEFLGAHLIGANVTEMIAEVVAARKLETTGHEIIKTIHPHPTMSEAIME
                     AAAAAYGEVIHL"
     gene            34287..>34568
                     /locus_tag="PLM90_02010"
     CDS             34287..>34568
                     /locus_tag="PLM90_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88169.1"
                     /translation="MKTLLILLLAGIQVQVFAQFEEFWPINSEGWITEYNANGPPQAN
                     YRFYVEEDTLIGDYTYKVLMRDRINPYTASTTTKLFAYFREEDGVITYYD"
BASE COUNT        10429 a         6846 c         7236 g         9857 t
ORIGIN      
        1 caggatgatg gaaaaataat tgccgcagga tatacctatg atataaatgg tgatgaatca
       61 attgcaattg ctcgatatgc ctcttgctcc gcttatttca ctttattccc tgatgcaacc
      121 ccgcacgtat ggaaggccgt aaacatggct gtcggctctg atccgatgag ctatgtgtgg
      181 gagtggggtg atggcacctc ttctacggaa ccatatccct cacataccta tgccgattcc
      241 ggttactacg acatttgcct aaccataaca gacggacttg gctgcacatc aacctattgc
      301 gattcatcaa cttacattta cagaatgtct gatgaacttg ctatggtaac tattaatgta
      361 tgggaagaat taccggtaga aacagaaacg ataactgctg atttttcagt cacacccaat
      421 ccattttccg atcaaattac aattgagatt ccgtcggctg tgtcaagcag tccgaatgca
      481 gaaatttcaa tatttgattt ggctggaaat atactattaa cgcaaatcat cagcgctcct
      541 caaacaacta tcaatacatc tcagttgcca accggtattt atgtgcttag ttatcataat
      601 ggactggagt ttggaacggc caagttggtg aaaatggaat gataacttga aaagtgcagg
      661 tgagcgtttc agactgctgt taaaaggtaa attgaggtaa tattattgtg gttgagtgtg
      721 gtcatgttgt tttccttact aatgttttta aaatattgtc agaattgata ggccctgttt
      781 gtattcaatc aaagtaatct cttcaccgat ggcattacca atatgaattt cattgttgat
      841 gttgtttttt aaggcataaa caaatactga catatcattg atttaagggt aaaaaaaaga
      901 ccgaccagca tgctgctggt cggtcttaaa taattgtttt cgatgtagcc caaaagatac
      961 cattatcgaa ccaaatagtg ttgagaatga aggagttaag ggatttcaaa tgcaattttt
     1021 tcgctaatat tacaacgcct ttacaaatca ttaaccaaca atgagcttat tcgacaatat
     1081 aaaaaaggaa gaggttgaaa ttatcgcaac tgtcaagaaa atagaaatcg tctacactta
     1141 caaaggggac atccagaaaa taacgctcat ctgtgattca gaaaagctta aaaagggtta
     1201 cgaaacatat ttcaattact caactaaatg gtcgaaattt aaccttaagt ctggtgataa
     1261 gctaaaattt aaggcaattc tcaaaaccac cacagtaacg gttcataaag acgaaaggtg
     1321 ggtggatgac tggggcacat tgccaattca cttaaccgat atggtagatt ttattgaaat
     1381 acaaagccct aaacaaatta tgttgtttta accactctta attttttaac gttccaattt
     1441 taaataaatg tctaaaattt aaatcccaaa ttctatatat ttcaatggat gctatttatt
     1501 attcaaaacc tgcaatgaat tttattctat atagcaaaat taaggtggat tcatttatag
     1561 tttctcacaa acaatattat agtcgtaaac cattgatgtt acaccagaac ttgtataacc
     1621 aacagtaaat tggatatttg tactagcttt agcatagata gtcatacatc cgctaatgac
     1681 tcctgtagct gtactattag ttgcgcactg attaaaatta tttatattgt ttgaaggcca
     1741 tgtttttacc acattatcag tagcacctgt gtaggaaacc gtgaatggtc ctaaaattga
     1801 tgaggttgag gcctgagtgg tgatagtgca cacccaactt attctataaa atccatcagc
     1861 agcaggcgtt gtatatattg ttgttgcagc ttgcgctgca gatttatttg tcgcttgagc
     1921 aattgcatat tctgcaggga ctccattgct caccgtagca atattattat attttgcgac
     1981 tttcccattt acatctactt gaaattcact attatctccc acggtgaata gattgtcagg
     2041 agtcacgtct ttacctgcat ttatgtatac atggtcgcca gcatcattat atagcatagt
     2101 taaagcagct tctggcgttc cagcagtcct aaatcgaaat cctactcccg aagcttgatc
     2161 atttctgata tcgtcgatat atgcaaaatt tccagaataa tcgaaccata cattaagacc
     2221 tctatttcct gaagcgttct gcattagtat tccggcatct ttatttgttg catttgattc
     2281 aattgaaaaa aacctactat ctgcagttgc atcccaacca tttggcaatg aggtattgaa
     2341 tgaactatga gttaatccgg catttccctc aggatcaatt agcatacttg tatgaattgt
     2401 ggtcgaatta attggtgtag tccgcactag agcataagtt ccttgtccag atccatcatg
     2461 tgtttcagct gcataaattc caaaaacacc acgatttgat gtttcaaatg cacttccagt
     2521 ataacctttt cctgaaagct caattaatcc ttgatctaca acagttgcct gaggggtgcc
     2581 tttactacca gaggctgttc taccagttac agtagcaaaa tgacaatcgg gataaacgca
     2641 atctgttcca ccataggtag taatattaat tccgccatct tgtgcggggc tgtcatcatt
     2701 ggagtcatgg tatatgttga aatccaattt ggggtcaaat tcttcacaat cagatagcac
     2761 taatttaccc agactggtaa attgaaacct ttgagttccg gaaaaagaac cattaatagc
     2821 ttcacctgta acccaactca gtccagttgt atgtgccccg gaaaatgcta atgaaggatt
     2881 agctgtagtt ccatttgata aaaatagttt atcaggaaat tccttatctc catcgccgaa
     2941 tacctgggta gatcctgtat tattgctaat aataccttgc gttcctgacc ccactattgg
     3001 taaatctgtt gccaatattg atctgaaact gggggttccc gacatcccat ccggtgaagc
     3061 gaaaaataaa ttttgagatt gactgtcaaa ggtcaatgtg agagtacccg ttgttgtcac
     3121 aggtgaacct gaaacatcaa acactgcagt aggagcagca agtcctacag aggtaacttg
     3181 agcttgcaaa taaatgcaac tacaaattag aataaataaa attgaaacgg gtttcatatt
     3241 acttaatttt gggttaaaaa ataaatttat ttaataattt caatagggaa tatataaacc
     3301 ttatctgata ctactgataa caaatacaat cctgaaaatg cctcaggtaa agacattttt
     3361 atttcatttg aagaaagaaa agtagaataa taaagcaatt ctccagttat tgagtaaatt
     3421 ttaattacta aaagttcaat aattgaggaa ttaacataga ttttgaattc tccagtattt
     3481 ggattaggaa atattttata aattgaggta tcaatatttt ctatataatt aaatccttca
     3541 tcaactgaac cattacagtt atcgtctacg ccgttttcta attcaattgc acctggatat
     3601 acaagagggt ttgaatcatc acaatcatca tcatttaaaa caaatcccat aattgtagaa
     3661 caagatatgg tgtcgattaa tggattacca taaccatcct catcgtaatc caaaaagtaa
     3721 gtagtgaatg ttgcgtcttc atcaagaata ccattacaat tgtcatcaat gtcattacaa
     3781 gagtcgcctt gatctgggtg tatcatggga ttaaaatcat tacaatcaaa attattttca
     3841 acaaacccat caggaatgta acatgcaact accgaatctc ccatagaacc ataaccatct
     3901 tcatcaaaat ctttatagaa agtgtatagt gtacaatcct caggataaaa tttaactatc
     3961 caataatcac cattcccaat gttatcctca tttttatcag gcgaaatatt tgaatcggag
     4021 gttcccccaa ctaaaaatcc accatcaggt gcttgaataa tacaacatgc aaaatcacta
     4081 ccagtgcctt gtagggaatt ctgccatgaa atattgccaa gtgtatctac tttaattatc
     4141 caataatctt ccattccata attaccctca accttattgc ccgtcgagcc tgataatgaa
     4201 tagccgacga gcgcaaaccc tttgtcaaat gtttgtataa taccttttaa ataatctgaa
     4261 ttatttccac caaaatcgtt ctgccattca acatttccta aggtgtctaa tgcaactatc
     4321 caataatcca aatcaccaaa gctttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnncatt tcctaaggtg tctaatgcaa ctatccaata atccaaatca ccaaagcttt
     4501 ccgaagtctt atctgctgaa atatttgatt ttgagcttcc acctgtaatt aatccatcat
     4561 tagaaattag atccaatgta taattgtaat cagcatcatt tcctccaatg gtgttttgcc
     4621 aaattatatt tcctatagaa tcaatttcaa ctatccaata atcatgtgtg ttcaaaccga
     4681 acggattgag aatacttgct tccgttttat cacctgaaat accagaattt gagttcccac
     4741 ctattaaata atcacctttt tcggtttgca ttaattcata taattcttca aaatttaatc
     4801 ctccaatcgt attttgccac aatatatttc caacactgtc aagcttaacc acccaataat
     4861 ctgtccctcc aattgcattt tctgttttgt ctcctgagat atttgaactt gacctccccc
     4921 caatcagaaa tccatcatca cgagtttgaa ttatattagt taattgatca cttgaagatc
     4981 ctccaatcgt attttgccac aatatatttc caacactgtc aagcttaacc acccaataat
     5041 catcgcttcc aaaatttaat tctgatttat ctccggaaat acctgattca gaataaccac
     5101 caaccagaaa tcctttgtcg tttgtttcaa taacagatgt taaaatatca gtcagcgatc
     5161 ctccaatcgt attctgccac aaaatatttc caacactgtc aagtttaact acccaataat
     5221 ctaaatatcc gatattatcc tctgatttat ccccgaatat tgttgatcca gatgaaccac
     5281 caattatata tccaccatca tggcataagg aaatacaaga taaattatca gacgaacctc
     5341 ctccaatcgt attctgccac tcgatttccg gagcttgaga atatgcgaac tctacaaaga
     5401 atgcaagaac aataaataat attaatttca ttattcaatt aattaaaata accaagtttt
     5461 attctttaac tactttttga ataaactcat ttccatctgt aattattttt aagatataaa
     5521 taccagaagg atatttactg atatcaaatt ttgatagtcc attttcaaaa agaaggtttg
     5581 tttgtaaata atatttccca gaaatatctg taatagagac agctcgagtt ctcgtaatag
     5641 tattttcaaa actgattact atatttccgc ttgtcggatt tggatatacc ctaatcaatt
     5701 ttaattgatt atttattaga ttaccttcct tcatgggtaa agttataaat gtttgaattt
     5761 cggaaaaatc gcttgactca ggtccgcaat tacttttcac cttccattcg tatgtagtga
     5821 aaggagttaa cccgggcaaa tttttattat tggaaaccgc tgtttttttt gtccagggcc
     5881 ctgtacctga aatacggtaa taaactttat aagaatctgc cgtcggaatt attgaccaat
     5941 gcactttcgc agaagtagag gtaatattat ctgagaataa acccgtagga acgccgcaaa
     6001 ttaatacgct atcacccatt aatttaatta cccaataatc tgtgcttcca agcgaaactt
     6061 ctgttttatc tcctgaaatt cccgaagatg aatagccgcc aactataaaa cccccatccg
     6121 aagtttcatc aattgtatta ggtgattgaa ttaaatcacc actagagcca cctatcacat
     6181 tttgccaaat tattgttccg gttgaattta atttcagaat ccaatagtca ttcccaccaa
     6241 ttttcgcttc agtcttatct ccagaaataa ctgaccggga ataaccgcct aaaaaataac
     6301 cgccatcaga tatttgttga attgaatata aatagtcagc atctgtccca ccaattccgt
     6361 tttgccaaac aatgccaccg gttgaattta gtttaaccac ccaataatca taattgccaa
     6421 taccggcatc ggttttatct gctgaaattc ctgataatga ccagccccct aaaatataac
     6481 cgccatcaag tgtttgttcg gcggaaaata aattgtcctc tgagtttcct ccaattgtat
     6541 tctgccattc aattacccct aatgaattta gtttgatcac ccaataatca tttcctccta
     6601 gacaagcttc tgatttatct ccagcgatcc cggaattcga gtaacctcca atgatgtagc
     6661 ctccatcaga tgtttgttta atagtattca gatattcatc agaccaagag cctatgtcat
     6721 tttgccacac gatgttaccg gctgaattaa gtttaactac ccaataatca gtgagtccaa
     6781 aattattttc agttttgtct cccgtaatgt cagattttga ttcgccccct aaaatataac
     6841 ctccgtcaga agtttgttga accgaatgta aataatcgtc taaagtccca cctattgtat
     6901 tttgccaaac aatgccaccg gttgaattta gtttaaccac ccaataatca aaaccaccga
     6961 tcgagttttc agttttgtca cctgaaatta gggaattgga ataacccgcc aagatataac
     7021 ctccatcaga agtttgttga atgcaattca gataatcatc tgaacttcct ccaattgtgt
     7081 tttgccactc aataaaaccg gcagagttaa gttttatcac ccaataatct tgaccagcat
     7141 gagtttccgt tttatcaccg gaaattattg atttggaaga cccaccgaga atatagccgc
     7201 catcacttgt ttgctgtata gaatttagta catcatctga gctacctcca atagtatttt
     7261 gccagtcaat acttggtgat tgtgcaaatg tgcaacttgc acataatgag atatttatga
     7321 acaatatttt aaacttttca gccattcagt tggttttacc aaactaaagt tatttttttt
     7381 aattggtaaa agcaactcac aagatggatt tattagtaaa attttcgaaa aaatcatgat
     7441 gattccaaat ccttcaacac tttgaggcag tttataacag cactaaaaaa tatcatatga
     7501 ataattggta agaaatccga tttactgcgg gaaaatttta atcaaagcat acaggaacga
     7561 gccggaagaa atagtagggg gaatacatga acctattgtg agcgaacagg tgttcgatac
     7621 tgttcaaaat gtattggcga ataagaagaa aattaaggcg aagcctaaaa ctaaatcaga
     7681 ttttctaccg cttcgcggat ttttggtatg tcctgactgc ggaaaaaatc taacaggcag
     7741 cgcatcaaaa ggaaatggcg gcaggtattt ttattaccac tgccagcccg ggtgttctca
     7801 aagggtgaaa gctgatgtcg ttcataattc attttcggaa tggttggatt cattttcctt
     7861 taaaccggaa gtagctgagc tctacctcgc tatcattgag gacactttca aacatgatga
     7921 agagggtcgt attaaagaga tcaagagatt aaagtgtaca atagatcaaa ttagctccaa
     7981 cctgctaatg gtcgaaaaga aatatgtcaa tgatgagatt gaacgagact cgtatctccg
     8041 tcttaaagaa tcatacaagc aggaagaata tgaattaaaa gaacatctcg tagaattgca
     8101 gtctgcggat tcagaataca tgaaatacat gaaatatgga tgttcagttt tatcaaacct
     8161 ccgccactgc tacgatacag ccgatgttga tgcaaagcaa aaactcattg gttcgatatt
     8221 tcctgaaaaa ctcattattc agaatggaaa gtatcgaacc acaaaagaga gtgaactgat
     8281 gcggctgctt tgcttgtccg gtgcggattt cggaatatct gaaaataaaa attcccgcaa
     8341 aagtgcggga aaattcgcta tggtagcccg taggggaatc gaacccctgt tccaagaatg
     8401 aaaatcttgt gtcctaaccc ctagacgaac gggccttgtc cgtaaaagag agtgcaaaag
     8461 tataagattt ttgcaaaaca gggaaatatg tcaccaaaaa ttttgttccg acagcctacc
     8521 tttgctccca ttatgagtac taactatcgc gtggccatca acggtttcgg tcgtattggt
     8581 cgcatcttct tccgcagagc attgctcaaa gcaaatttcg aaatcgtggc tatcaacgac
     8641 cttaccgacg tggataccct cgcccatctg ttgaaatatg attcagtgca tggcagattt
     8701 cagggtacgg tgtccgtgga aaacggtaac ctggtagtga atggcaagac cattcgtgtt
     8761 tccgccatga aagacccggc gcaatgcccg tggaaggaac tcaatatcga cctggtgata
     8821 gaatctacag gcctcttcct ctcacgcgag aaggctgagc tccatattca ggccggtgct
     8881 aaaaaggtat tgctgtcggc ccctccgaaa ggcgatgata taaaagccat ggtgcttgga
     8941 gtaaacgatg aaatcttatc gcctgccgat aaaatcatct ctaacgcttc ctgcactacc
     9001 aactgcgcta cgcctatgat caaggtgatt catgagcatt ttcatataga atctgcttat
     9061 atcaccaccg ttcacgcttt caccagcgat cagcgcctgc acgatgcgcc gcataaggac
     9121 ctccgcaggg ctagggctgc tacattgaat atggtgccta caacaacagg tgctgctaaa
     9181 gccgttaccc tggccttccc cgaactgaaa ggtcgcgttg gcggcgccgg cattcgcgtt
     9241 ccggtggctg atggctctat tacggatatc acttgcaccg taaccaaacc aacaaccatc
     9301 gaagaggtga atgcgaaatt taaagaagcc agtgaaacat acctgaaagg tatcctctcc
     9361 tatgaaacag atcctatcgt ttctgtcgac atcgttggcg atccacattc atgtattttc
     9421 gacgcagagc ttacttctgt tatcggcaat atggtgaaag tgctgggatg gtatgataat
     9481 gaatatggtt attcaagcag attggcagat ttgaccgaga gattggcaac aatggcgtga
     9541 attggtagtg aatggtgaat ggtgagcctg cctcgccgat aggcgggtgg tgaatggtga
     9601 gtggtgaatg gtgagcctgc ctcgccgata ggcgggtggt gaatggtgaa tagtgaatag
     9661 tgagtggtga gtagtgaata atacttgata cctgatacct gatacttgat accaattcaa
     9721 gataccaatt ctcagtgaat gttgagcaat taactgataa taaaattact tggagattta
     9781 attaaaaaat cattataaaa atgagaacga ttcaggattt taattttgct ggaaagagag
     9841 cggtgatacg tgtcgatttt aatgtgccgc tcgataaagc aacattcgct gttaccgacg
     9901 atacacgcct tcgcgcagca ttgcctacca tcaataaaat attacatgat ggtggttctg
     9961 ttgtgttgat gtcgcatctt ggccgaccaa aaggcggacc tgaagataaa tattcactgc
    10021 gtcatatcgt tgcgcatttg tcgcaattgc tgaatcgcga tgtgcaattt gtaaatgatt
    10081 gtattggcga tatggtaatg tcggcagcca ggcaattaca accgggacaa gtattattat
    10141 tagagaattt gcgtttccat ccggaagagg aaaaaggtga tcatgcattt gcagaacaac
    10201 tgtcgaaatt aggagatgta tatgtaaacg acgctttcgg aactgctcac cgtgcgcatg
    10261 catctacaac aattattgcg aattatttta ataccggaaa taaaatgttc ggcgccttaa
    10321 tggcagctga agtaagcgca ggaaataaag tattgcatca acacgaaaaa ccttttaccg
    10381 ctatcattgg tggtgcaaag gtttctgata aaattctcat cctcgagaaa ttattggaga
    10441 aagcagataa tgtaattatc ggtggtggaa tggcatatac tttctacaaa gcaatgggcg
    10501 gacaaatcgg aaattcattg tgtgaggaag aaagattaga tacggctaaa caattattag
    10561 aaacggcaaa agcaaaaggc gtaaatatat tattgcctgt tgacagcatt acagcggata
    10621 aatttgataa tgatgccaat atcggatctg ccaacagcga tcagattcct gatggttgga
    10681 tgggattaga tattggtgaa aaagcgattg ccgattttac agctgcaatc ctcaagtcgc
    10741 gcacaatttt atggaacgga ccaatgggcg ttttcgaaat gtccaatttc tcaaaaggaa
    10801 caattgccat cgcacgtgca attgcagaag ctacagctaa tggtgcatat tctcttgttg
    10861 gtggcggtga tagtgttgca gcagtaaata aattcaatct cgccgataag gtcagctatg
    10921 tatctacagg tggtggtgcc atgctcgaat ttttcgaagg aaagaccttg ccgggtgtgg
    10981 ctgcgatagc agattaaaaa aaactttcta atcgcttacg cccttatcgt tagcggaaaa
    11041 ttaaaatgac ctccttcggg aggttttttt atttgtatct gctgtttaca tttatcgcct
    11101 tgtttacccc ttatgcctga acattggcac catattcctt ttctgcgttt tctactccca
    11161 cttgtttgtg gcgtattgat cgcattgcaa tttcatgagt gttattggtg gatttacgga
    11221 ctctttttcg ttttatttat tgcatatcta ttaatcggca tagttacagg ttggactgta
    11281 aaattccgaa gccagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctg
    11401 tttatggcga ttacgcatta attgaaaatt gtttcaaatc tccggacccg ccgaaaaatc
    11461 ccaatacctt tgatttccca aaatatttac gtggtattgg catcgcacat attgcccgcg
    11521 tagatgcatc gcaattggtg tttacaggtg agcgacatca aaaattgtgg tggacattta
    11581 tttttcgtgc gagagaaatg tttcatgatg ctgtgatgcg cgaagtgaaa aatggcgatg
    11641 ctcaatctgt tggcgaagca ttagtgatag gcacaaaaac tgccattgat ccggaggtgc
    11701 aacaagctta tgccaataca ggcaccatgc atgtgctggc agtttccgga ttgcatgttg
    11761 gtattttatt cgcggtattg gaattcttat taaatccaat atcatggttc aaaagaaata
    11821 agtatggaag ggtgtcaaaa ttcttattta ttattacaat aatatggtgg tatgcgtgct
    11881 tgaccggttt atctgccagc gtaaacaggt ctgccgtaat gttcacatgc ctggctaccg
    11941 gaaaattatt cagcaggcaa aataattcgt ttaatgtatt atttgtgtcc atgtttttat
    12001 tattggtaga tgatgcccga tgtatcacac aggtgggatt tcaactatca tatctcgcag
    12061 tgggcgggat actttttttt caaccttatc tgcaaaaatt atggcgaccg aaatggaaga
    12121 ttttaaatca tatctggaca atgacgactg tttcagtagc cgcacaaatt agtacatcgc
    12181 cgatatctct gttttatttc catcagttcc cgaattattt cttgttgtcg aatatgctgg
    12241 caatcccaat atcatttgtt gtgctggttt tgggattgat atttttcgtg gtgaatggaa
    12301 ttccgttttt gggaccggca acaggtatgg ctttagatta ttccctgcga ttattgaatg
    12361 gttctgttat ggcagttaat cagatgccgg gagcggtgat taataacatt tatatcaatg
    12421 aatggcagtt aggcatcttg tatgtttttg tgctgctatt gggaattgca cttttgagca
    12481 agcaaactat gtatctgttt cgtgctgctt tttgtttatg tatcgtttgg atgctggctg
    12541 tttttaggga tgttaaggcc cgcgaagaca ggagattggt tgcctacagt atcagagacc
    12601 acgactgtat gttgctgcag ggggcgggtg atgccatttt attatctgat accctgatcg
    12661 accaaaacag ctccatttgg acactgaata tcaggaatga tgcattgaag tctggtgtta
    12721 tgcccgattt tgcccttttt agccttcagg agccttttaa cggggcttct aatgtcctat
    12781 acggctatcc ttggctaatc ggagggggta aggtggtttt tctgcttgat cccacttctg
    12841 tacaccaact gccaaaaagc gcccctgaag cagactgggt gtacattctg ggcaatccct
    12901 tcatcaacct acccgacctg caatcgcgct tccccaatgc caccttcgta atcggcggaa
    12961 gtactagtta caagcggcat tcatactatt tgaatacctg taaaaagctt caaattcccg
    13021 tccattccct tcgattcgac ggagctttgg tattggaatg aagtagcctt gctgtgtaag
    13081 tggtcctcca aagcgggcct catcgctact ttatgcacag ggtgtgaaaa gctccctaca
    13141 aaccaagctc aaaccctttc ctgcagcgta tttcattgat tctcacattt gtgtataaac
    13201 catgtgcatt ccgtgtgcaa aaattttcaa agcaccgaag tgtacaaaag tgtacaaaag
    13261 tgtaataatc tgtctaagtt tacgttcaaa ttagctccaa gaccagcaat atggcattcc
    13321 tgatcggtga atatgagtgt actcttgacg tgaaacaacg tatccgcgtt cctgcggggc
    13381 tgttagggca gctcacaggc gatgacgccg gaaagttcgt gatcacgcgt ggtattgacc
    13441 catgccttta tctatacccg atgagtgaat ttcatataat aatggacaag gtgaaccaga
    13501 ttccggagta ttcggaggag gatagggagt ataagcacat gtttttcagc ggtacttcca
    13561 ttcttcagat cgattctgca gaccgcattt tgttgccgaa gttgcatttg gcgcatgccg
    13621 gtattgtaaa ggaaatggtg atgacttgcg agaagaacaa agtggtggtt tggtctatgg
    13681 agcgattccg tgagaagttc ctcaaccgct cttcactcga ttaccagcag cttgctgaaa
    13741 aagtactcgg caaatatggt ttatgagcga ggcgtatcac atacctgttc ttttacatcc
    13801 gagcatacag gcattgatca ccgaccccga tggtatctat gtagatgcca cttttggagg
    13861 cggcgggcat agcaaggcga ttgtggagca gctttctcca aaggggaaat tattctgctt
    13921 tgaccaggat cccgatgcaa aaaaaaattt catcgatgcg tttagcgcta ctaacgcaac
    13981 tttcattccg cagaacttcc gccatatgca gaagtttctg cgggtgaatg gtgtttcaaa
    14041 ggtgcatggt attctggcag atctgggtgt cagctcgcat cagttcgata ccggcgaccg
    14101 cggtttttca atacgtttcg atggtccgct cgatatgcgg atggcacagc agggaacaca
    14161 atcggcatac gccctgatca atgaaagcag cgctgaacaa ttgcaataca tttttggcca
    14221 ctacggcgaa gtacgcaatg cacggcaact tgccaatgcc attgtgcaaa caagaaatct
    14281 cttcgcagca accggcggta ttcgcaccac cacacaattg cgagatgtgg cgctgacagt
    14341 actcaaaggc gaaaaaaatc catacctggc tcaagtattc caagcgcttc gcatcgccgt
    14401 gaacgatgag atgcgcgcat tggaagaaat gctcgaaagt gcagcttcat tgctgatgcc
    14461 gcagggcaga ctggtggtaa tcagttatca cagtcttgag gacaggctcg tgaaaaatta
    14521 tatgcgggca ggaaatgcag caggtgaagc tgaagaagat atcatgggca gaaaaaatgt
    14581 accatttaag gtgatcacca gaaaacctgt ggtagccgat gacgaagaat tatcaataaa
    14641 tccacgcgct cgcagtgcaa aaatgcgtgt ggcagaaaaa atataacgcg caaaccaact
    14701 atggcgcaac aagaaaaaaa taataaagga aaggaacaaa aaagaagcct tgcatcaatg
    14761 ctgaaatcat ttgatgaggt tgcttttttt aagtgggaaa atattctcgc gaatatccct
    14821 tatttcttgt tcctcgtagt gatcggtatt ttttatatat ggaataatca ccgcggtgtt
    14881 gctatggatc gcgatatccg tgatctgaac acgcatctcc tcgaaaaaca atatttctat
    14941 aacgccacaa gcgactctct cacacaacag agtcgccaaa gcacagtggc gcgcatggta
    15001 gacacactgc agatgcaaga attatcgcat ccgccatata ccattaaaac gaacgatgga
    15061 aaacactaag aaagacatat tatggcgttt atacctcacc ttcgcaggta tagtattgct
    15121 gtgtctggca attattgcac agatcgtcaa cgtgtctttt gtgcatggtg aaacctatcg
    15181 tgcaatggcc gacagtgtgc gcacgaaaca agtgagcatt ccgcctgctc gtggttctat
    15241 ttattctgat aaacatgagg tgctcgccac atcatttcca tatttcaata tttatatgga
    15301 tccatgtgcg gcatctgaag tagattttcg tcgcgatgta gattcactga gtattgctct
    15361 cgctcgttta tttaaagata aacctgcctc cgcatataaa cagaaaatta ctacagcacg
    15421 caaacagggt aaaaaatatc tcgagattca taagcgcgtt accctcccgc aaaaacaact
    15481 catgcaaaca tttccattgt ttcgcatcgg gcaaaatgga ggaggattta ttgtagagcc
    15541 gtatgaaaaa cgcgagtatc catatggcag tctggcagtg cgtaccattg gctacgtacg
    15601 caatggaaga ggtattggtc tcgaaggtgc ctatgacact ttattgagtg gtaaacccgg
    15661 catggctgtg atgcgcaaag ttgcaggtgg ttcatggatt agattaagcg agcaaaatga
    15721 aatcgatccg gtagatggtg tagatatcat caccacactc gatatcaata tgcaggacat
    15781 caccgaaact gcactgcgca atgcattagt aaaaaataat gcaaagtggg ggacagcagt
    15841 ggtaatggaa gtgaaaaccg gaaaaattaa agccatttca aatctcagtc gcgaatcgga
    15901 aggtgtttac catgaggatt ataattatgc catcctcgaa aaagtggagc ccggttctac
    15961 atggaaatta ttttcattga tgtgtttgtt tgaagatgga ttatctatca atgatcatgt
    16021 cgatttaaat tttgggcaat atccttttgc cgatcgtatc atgtacgaca gcgagccaca
    16081 tggcagacgt gatgttacgg tgaagactgc ttttgcttta tcatcaaatg taggtatatc
    16141 aagactggcg tgggatatgt atcaaaaaga tccggagtct tatgtaaaac atttacaaga
    16201 atccggcata gtgaatgtaa ccggtgtgga attaccggga gaacctaagc catatttcaa
    16261 acaagatcca tcagataaaa accattggta taaaactact gtgccatgga tgagtgtcgg
    16321 atatgaattg ttgatcacac cattgcaact ggcaactttt tataatgctg tagccaatga
    16381 cggtgtgatg atgaaacctt atttggtgga agaaacacaa cagcatggta aaacattgca
    16441 gaaattcgaa cctgttgttt taaatgaaaa actttgttca agcaaaacaa tcgatcagtt
    16501 gcatcagtgt ttgttggctg tagtggatag cggtactgct aaacatttga aaaacgatta
    16561 ttacaaattc gcaggtaaaa caggtactgc gcagttgctg gaaaatggcg tttacggtca
    16621 taattacttg tcaagttttg caggttactt tccgtatgat aatccaaaat ataccattgt
    16681 aataataatt aactcgcctt caaacggtcc attttatggt ggtgctgtag cagctccggc
    16741 tttcagggaa atcgcggata aggtgtattc gcattttgta aatatgcgag agccggtaaa
    16801 tgcgccaagt gctgcaaata ccggtgtaca ggccttttca aagggatatg cctacgacta
    16861 tcgtcagatc tttaaaacac tcaaagtgaa tgaagatttt aatgataatg aagaatggat
    16921 agccgtttct gcaaatggaa ataattcgga acatacagaa ttaaaaacat atcaatatac
    16981 gatgcctgat gtgcgtggaa tgggattgcg agatgccatg tatctgttag atacctacgg
    17041 tttaaatgtt gctgtttccg gagcaggaaa agttaccatt caaagcatcc ctcccggaac
    17101 agccattcaa aaagataatt atgtaatgct aaccctggaa tgaaatcatt aaaagacata
    17161 tcatctcagg tgcgcatatt gtcgcaacac ggtgtaagcc tcacgtcggt tcagattatt
    17221 ggtattacag ctgatagtcg ccgcgctgca gaaggtatgt tgtttgtagc cgtgaaagga
    17281 actactgtag atggatatga ttttattcca accgccatcg ctcagggagc tgttgctatt
    17341 gttgctgaaa gaaaaataga ggtgccggat aatgtatgct tgattattgt agacaatgct
    17401 gcagctgctt taggaataat ggcgcatgca ttttatgatt acccgtcaac aaaattaaaa
    17461 ctcgttggta tcacaggtac aaatggaaaa actaccacag caacattgtg ctatcatgta
    17521 atgcgtgatt taggttttaa tacgggtttg atttccacgg ttgaaaataa aattaacgaa
    17581 gaggtaatac cttcaacaca taccacacct gatccggtgc aattaaacgc actccttgct
    17641 aaaatggttg atgccggttg cgcttatgca ttcatggaag taagttcgca tgcagcacat
    17701 cagcatcgca tagcgggact gcagtttgtt ggaggtgtat ttactaatat cacacatgat
    17761 cacctcgatt atcacaaaac attcaaagat tatatcgacg cgaagaaaat gttcttcgat
    17821 ggcctgactt cagatgcatt tgcacttgta aactccgatg atcgcaacgg aaagatcatg
    17881 ttgcaaaact gtaaagcgaa gcatgctact ttcagcgttt taggtacagg tgattttacc
    17941 gctcgtatta aagagaatgt gatcagcgga ctcgttttgc agttggatgg agtggaattt
    18001 cactcattgc tattaggtga attcaatgcg tggaatattc ttacggtata cgccatattc
    18061 agattactcg gatttgaaaa acaacaagtg cttaccgtat tgagtgctca aaaacctgtt
    18121 gaaggaagat ttgatgtgct ttataccggc gacagatcaa ttaccggtgt gatagattat
    18181 gcacatacac ctgatgccgt tgaaaaatta ttgtcaaccg tgcgatcaat gctaaaaaaa
    18241 gaccaacacc tcttaactgt tgttggttgc ggtggcgatc gcgatcgtgg aaaacgacct
    18301 gtgatggctg ctacggcggc aaaaatcagc gataaaacaa ttctcacctc cgataatccc
    18361 cgaagtgaag aacccgatgc cattattaaa gaaatggaat ccggtttgaa tgaagaaaca
    18421 aggaaacaca gcttgtcgat cactgataga aaagaagcga tacgcactgc tgttatgctg
    18481 gcgaaaaaag gtgatgtgat ttgtgttgtt ggcaaaggac atgaaaaata tcaggagata
    18541 aaaggagtaa aatatccatt cgacgataaa gaagtattgc gcgaaacatt taaaaccttg
    18601 gcccactaat gttatactat ttatttaatt ggcttgatca acaatacgat gttcccggtg
    18661 cggggatgtt tcaatacctc tcgttccgcg caggtttggc cgtattatta tcgttgatct
    18721 tctccatcgt tatcggcaaa cgactcatcg cttatctgca gaaaaaacag attggcgaaa
    18781 gtgttcgcga tcttggactg gaaggccaat taagtaaaaa aggtacacct acaatgggtg
    18841 gtataatcat tattctttca ttgcttattc cggttttatt acttgccgac ctgaaaaata
    18901 tttatgtcat cctcatgctc atcgtaactg tttggatggg attgatagga tttttggatg
    18961 actatattaa agtattcaga aaaaataaag aaggcctccg tgcaaaattc aaactgatgg
    19021 gtcaggttgg actgggagct attgttggac ttgttttgta tttcagcgat gacatcaaac
    19081 tccgtgatta tgacatggta tatgtatatg ctgttgaaaa tggacagcct gtcggcaggc
    19141 caatagatgc ctatcctgca aatgacacac gattggaaag tgatggtatt ctgaattctg
    19201 acagcacgca ttttttaagt cacgatacca gatcaacaaa gtctacaatt ccactcgtaa
    19261 aaaataatga atttgattat gagtggttgg tgtcatgggc atcgccggaa acacagaaat
    19321 ggctattccc gatagttttt gttgctataa ttataataat tatcactgct gtatccaatg
    19381 gtgcaaatct caccgatggg ctagatggat tggcggcagg cacatcaact attatcgtac
    19441 ttacattatt aattttcgcc tatatcgcgg gtaactccat tcttagtgat tatctgaata
    19501 tcatgtacat acctgactcc ggagaattgg tagtattcag cgcagcattg atcggagcct
    19561 gcatcggatt tctatggtat aacgcctttc cggcacaggt atttatgggc gatacaggaa
    19621 gtttgacatt aggcggtatc atcgcttcat tggcaattgt catccgcaag gaattattaa
    19681 ttcctgtttt atgcggaata ttcctggtag agaatttatc tgtaatgata caggtgagtt
    19741 atttcaagta taccagaaaa aaatatggtg aaggaagacg catttttctg atgagtccac
    19801 ttcatcatca ctatcagaaa aagggcatgt ttgaaactaa gattgtaaca cgtttttgga
    19861 tcatcaccat attattagct attgtatcca tcgtaacact taaagtgcgt taatggcaaa
    19921 aaagctcgtc atattaggct ccggtgaaag cggaacaggt gctgcaatcc ttgcacaaca
    19981 gcaggggttt gaggtatttg tttccgacaa ggggaaaata cgcgacagct ataagcatga
    20041 actttcttcg cgcggtatag catttgaaga aggtaaccat tccatggatg taattcttgc
    20101 tgccgatgag atcattaaaa gtccgggcat acaagagaaa tatgatgtga tgaagcaaat
    20161 tcgcgcagct aataaaattg ttgttggcga gattgaattt gcatggcgtt attgcaaagg
    20221 aaaggtagtt gctatcacgg gaagcaatgg aaaatctacc tgtacttcgc ttacctacca
    20281 catgatgaaa aaagcaggtt gcgatgtggc aatcggaggg aatatcggta agagctttgc
    20341 tgaattggtt gcgcatacca atcatgaata ttatgtattg gaagtaagca attttcaatt
    20401 ggatgatgct gttacattca aaccgatgat cagtgtatta ctcaatatta cccccgatca
    20461 tctcgatagc tataattatg aatttcaaaa atatatcgat tcaaaattcc gaatcaccat
    20521 gaatcaaacg gatggcgatc atttcattta ctgtgccgat gacccggtaa caagtgaaga
    20581 aatgctaaaa cgtcattctc catttttgag taacccggcc aacttactca cattcacgct
    20641 ggatgaccga cagcatgaag gtgcatgggt ggctgataat tccattcata ttcaaatcaa
    20701 caaaaaccac ttcacaatga ccattaatga attagcgcta caaggtaaac acaaccagta
    20761 caacagcatg gccgcgggaa tcgtaggcaa agtactggga ttgaaaaatg aaagcatccg
    20821 tgagagcttt acagatttca gcggactcga acaccgactg gaatttgttg caaaagtgca
    20881 cggcatttca tttattaacg attcgaaagc aaccaatatc aacagcacat ggtatgcact
    20941 tgaaagtcag gcagaccctg tcatctggat agttggtggc atcgataaag ggaatgatta
    21001 ttctcttatt gcagatctcg tgaaaaagaa agtaaaagcc attgtttgtc tgggaaaaga
    21061 caatacgcgt attcacgaag cattcggaaa aatggtggat attcttgtaa acaccgaaac
    21121 aatggaagat tgcgtgcaaa tggcttatcg ccttggcaaa aatggcgata tcgttttact
    21181 gtcgcctgca tgtgcatctt ttgatttatt cgaaaatttt gaagatcgcg gcaaccaatt
    21241 taaaaaacag gtgcgcttgt tgtaatcctg tggtcattta attcaaccat caccaatagc
    21301 gtttatgtct aactcaatac aatatctgtt cagcagagtg agaggcgacc ggtacatctg
    21361 gttggtggtt tttgtattgt ctgtattttc cattcttgca gtgtatagtt caacagaaac
    21421 acttgcatat cgttcagccg gtggaaatac agagtattat ctggtcaaac actcggtcat
    21481 cttgttgttt ggtatcgtgc tgatgtatct cagtcacctc ataccatata aatattacag
    21541 cagaattgcc cagatattat tatggctgag cgttccggca ttactctaca ccatgtttct
    21601 tgcaccggaa gtaaacgatg catcgcgatg gatcactttg ccggttatca atcttacgtt
    21661 tcaaacatct gaccttgcgc gttttgcact catcatgtat accgcgcggg tgctttcgaa
    21721 aaaacaagat aaggtcgatt cgtttaaaga agcttttatt ccggtaattc tgccgatagc
    21781 aattatttgt gcattaattc tcccggaaaa tttatctact gcgagcgtgt tattttttac
    21841 atgcatcgtg ttgatgtttg tgggtagagt gaaattcaaa tatatattac tcaccatcgg
    21901 cgcaggtatt gtgttgatag gattaatcat tgcattggca tatgcatttc cgcacgtggg
    21961 acgtatgggt acctggaaag cgcgtgtaga tagctttgtt gacggtggaa cttctgatga
    22021 tacctatcag gtagaccaag cgaaaatcgc catcgccaaa ggaggtgtgt tcggtgttgg
    22081 accgggaaat tctacacaga aagatttcct tccgtatcct tatgccgatt tcatttttgc
    22141 aaccattctc gaagaatatg gattaatggg aggcatattt gtgatatttc tgtacttggt
    22201 tttcttatat cgatgtatca aaatcgtagt aaaggcacct actagttttg gtgcattgct
    22261 ggctgtagga ttgggactgt gtttgtgtat tcaggcattt ataaatatgg gagttgccgt
    22321 tcacttactg cctaccaccg gtttgacatt gccattagtc agcatgggtg gaacctcttt
    22381 gtggtttacc agtcttagca taggtattat tttaagtgta agtagagaaa ttgaaaaagc
    22441 tgatgaagtt gaggaggaag atgaacaaac ggaaactgaa gaacaatctg aagaagaacc
    22501 ggaattagca aaatagatat taaataagaa gcattggcat catcaatcaa catattaata
    22561 agtggcggcg gaacaggagg ccatattttt cctgccattg ccatcgcaaa cgaaataaaa
    22621 cgacgtaggc ctgatgccaa tattttattc gttggtgcgc aggataaaat ggaaatggag
    22681 aaagtgccaa aatcaggata tccgattgaa ggcttgtgga tcagtggctt tcaccgaggt
    22741 ttggattggc gaaatttaat gtttccgata aaattgattt ccagttattt caaatccggc
    22801 agcatcgtaa aaaaattcga accggatatt gccatcggaa ccggtggatt tgcaagtgga
    22861 cctgctctga atgcagcact tcgtcatcaa gtgccgatca taatccagga acaaaattca
    22921 tatcccggag ttaccaataa aatactgtcg aagaaggcaa aagcagtatg cgtggcctat
    22981 gatggtatgg agcaattctt tcccgcagga aaaattgtga aaacaggaaa tccggtaaga
    23041 caaaatatag tgctttgcaa tgtgaaacct gaaaaggcgc gtgccgattt tgatatcgat
    23101 atcgatagca aagtattatt tgttgttggt ggaagtttgg gatcgcgcac gcttaataat
    23161 tgcatgatag gcggtttgca agaacttgca gatgccggca ttcaagttat ctggcagaca
    23221 ggaacggcca tgtatgaaat gtgtaaacat gctgcgaaag gatatgataa tgtccgggta
    23281 ttggatttta ttgccaatat cgatcacgca tatgccgccg cagatatcat agtttcaaga
    23341 gcaggggcta ttgccatttc ggaattatgc cttgtaggga aacctgtaat tctggtgcca
    23401 tttcctgctg ctgcagaaga tcatcaaaca aaaaatgcaa tggcgttagt gaaagaaaat
    23461 gccgccatgc atattgcaga taaatcggca cctgtagaat taataccaaa cgccattcaa
    23521 ttgatgcaag atcatgatag acggtcgtta atggcacaac gcattaagaa ttttgcaatt
    23581 gctgatgcag cagagcgtat tgttgatgaa gtatttaaat atatccaagc aaaataaata
    23641 atggatatcg cacaaataaa tagggtttat tttattggta tcggtggcat cggtatgagt
    23701 gcacttgcgc gttactttat gcgacaaaaa tgtgtagtgt ctggatatga tcgcacagaa
    23761 acagaaatta ccgatgcatt gcaacacgaa ggtgctcaaa ttcattttag tgaagatctt
    23821 acacaaatag acacccaagc agatctcgta atttatacgc ctgcagttcc gccaacgcat
    23881 gccgaattaa tatactatcg cgacaatgga tttaccgtgc tgaaacgcag tcaggttctg
    23941 ggtttgataa ctaaagacaa gtttgtgatt gcagtagccg gctcgcatgg taaaactact
    24001 gtatcgagta tgattgcctg gattttgaaa aatagtggtt acgattgcac tgctattctt
    24061 ggaggtatct caacaaattt taattcgaac tatgtcatgg gttttaacga tgtttttgtt
    24121 gttgaagccg atgaatatga cagatccttt ttgcaattat cgccaaatat tgctgtgatc
    24181 actgctacag actccgatca ccttgaagtg tatggtaccc aggaagcaat agaagcaaca
    24241 tttcttgaat ttgccaacag gataaaagaa aatggattgc tgatttcgaa acctcgattg
    24301 ggtgtattcc aaaaatatca gcgcgataag cgtggatatg acctgacaga tagtaaagcc
    24361 gatttgtatg ccgtaaccta cgcatatgaa ggggatcgtt gtcatgtcac attgaatgac
    24421 ggctatgaat tttacttggg atatccggga gtgcataata tcgaaaatgc catcgccgca
    24481 atatctgttg cactacattt gaatattcac ccggacttga tatcacgcgc tattacagcc
    24541 tttaccggtg tgcatcgtcg ttttgaatta gtgaacggag tttttgggaa aattccatat
    24601 tacgacgatt atgcgcatca ccctgaggag ataaaaatgt ttctcaccag tattaagcaa
    24661 ttatttccga ataaaaagat tacggcagta tttcagccgc atctctttac cagaacacga
    24721 gatcttgctg atggatttgc cacaagttta gatattgctg atgaggtgat tttattaccc
    24781 atttatccgg cacgtgaaga accaattccc ggtgttaccg ccaataacat tctcgaccgc
    24841 atgaagaacc cgcatcgccg catactcgag aaggctgatt tgattaaata tgtttccaag
    24901 gccgacatcg aagtgttttg tactatcggt gccggtgata tcgataaact tgttcaaccc
    24961 attgcagaga tcttaaaagg ccgatgaccg gaaaacaaaa gataatgcgc gttttcggta
    25021 tcctgctgct ggtagcatgc ggaacaggat tgctgttgtt aacggctttc aatgccgata
    25081 gacaggatgc aagcgcctgt atcgatgtgc aaattcatgt ggatgaagag cgcggtattt
    25141 attttctcga tgccaatgat atacgcacta tcctggcaac agaatacggc gattcggtga
    25201 aaggcgatgc actcggatca atacgtgttg gtcgcatcga aactttactc gaacgcaatc
    25261 catacgtcga tgatgctgaa acctgggttg atgcaacagg aaaattgcat atcgaaattg
    25321 tccaaaaaca acctattgct cgcgttatta acaagtatgg tgttaattat tatattaata
    25381 atataggtaa taagatacca atcagcgata aatttacagc ccgtgtacct gtcgttactg
    25441 gcagcataga agagggcatt cataacgcag acatggcaac aacctcggcg ctacaaaaca
    25501 tactcaaaat cacgcgtttt attaacgcag atccattctg gagcgctcag attgaacaaa
    25561 ttgctgtcgc caatgacggc actttcaccc tcatccctaa gcttggtgac cacaagatac
    25621 tgtttggagg tatcgcacac atggaagaaa aattccggaa aatgcaggtt ttttataccg
    25681 aaggcctgaa tcataccggt tggaatacat acagtgcttt tcaattggat tttgaagggc
    25741 agatcgtttg tgaaaaaaac aagaactatg aacaacagta accgtatcat caccggactc
    25801 gacatcggaa caacgaagat ctgcgctatc gtaggtaaaa tcaccgatca tggaaaaatc
    25861 gagatcctcg gcatgggaaa agcggattca ttcggcgtta tgcgcggcgt ggttgccaat
    25921 atcgataaaa cggtagaagc tatccgcaga gccgttgccg aagctcaaga gaaaagtggc
    25981 gtcgaaatca atgaggttta cgttggtatc gccggtcagc atatcaaaag tctgcaacat
    26041 cgcgacatcc tcacgcgcaa cgatgcagaa agtgaaatcg gaatggacga tatcgatcgc
    26101 atgattgaaa acatgcatcg ccttgtgctt cctcccggag atcgcatcat ccatgtgttg
    26161 ccgcaagaat ttattgtcga tggcgaacaa ggtatcaaag atcctatcgg tatgagtggt
    26221 gtgcgtcttg aagcaaattt tcacattatc accggtcaga tttctgcagc gaaaaatatt
    26281 ttcaaatgtg tagagaaagc cggtctcaaa gtaatagacc tcatccttga accattggca
    26341 tcttctgctt ccgtacttag cgaagaagaa aaggaagccg gtgttgcctt ggttgatatt
    26401 ggcggtggaa caacagatat cgccattttc caggatggta tcattcgcca tactgccgtg
    26461 atcccgctgg gtggtaatat catcactgaa gatatcaaag aaggttgcat gatcatgcgc
    26521 aatcaggcag aaatcctgaa agtgaaattc ggatcggcac tggcaacaga aactgaagag
    26581 aatgagatca tctcaatcag cggattgaaa ggtcgcgaac caaaagagat caaagttgaa
    26641 aatcttgcac gcatcattca agcgcgtgta gaagaaattc tcgaacacgt ttactatgaa
    26701 attaaattat caggcttcca caaaaagctg attggtggta ttgtaatcac cggtggtggt
    26761 gctcaactca aacacctcgt gcaactggta tcctatatca cagggatgga tgcgcgcgtt
    26821 ggatatccga cagaatactt gcaaaaagca aaagctgaag atattcgtca cccaatgtat
    26881 gcaacggctg tcggcttgat cttaaaaggt catgacgatc gcaattatcg tgagcgcgaa
    26941 attgaaatgc cggcacctca gcagcaacag gaaatcgtga atcctgttgt tgacaaaatg
    27001 ccgacaagca atgagcagct caatcaagtt gcagagaagg aaatgaatga tgcaaccatg
    27061 gaaatggaac aagagcttgc gcacgataaa catgctaaac cgagttggtt gaaaaacatg
    27121 ttgtcgagca cacgtaagtg gtttgaagaa gacgacgtat ctgattttaa ataatacccg
    27181 aacgtgaacg cgggcaatac cttgaaacac aacacacaat aaaacgaaaa cgaacctatg
    27241 tattttgata ttccaaaaga acaagcctct atcattaaag tgatcggagt aggcggtggc
    27301 ggtagcaatg ccgtgaacca catgtttttg cagggcatca aagatgtgaa ctttgtaatc
    27361 tgcaataccg accaacaggc acttgaaatg agccctgttc ccaataaagt gcagcttgga
    27421 ccaagcctta ccaaaggtcg tggtgcaggt tcacatccta ctgtcggcca acaagctacc
    27481 atggaaagct tgcaggaaat aaaatctatt ctcgaaaaaa ataccgagat ggtttttatt
    27541 actgccggta tgggtggcgg taccggaaca ggtggtgctc ccgttatagc gaaacttgct
    27601 aaagatatgg ggatccttac catcggtatt gtaacagtgc catttggttt tgaaggaaag
    27661 cgcagaaaat cgcaagcatt cgaaggcctt gaaacgctca aacattatgt agatgccatt
    27721 ttggtgatct cgaatgataa gcttcgcgaa atgttcggta acctggcttg gaacgaagca
    27781 tttagtcagg ctgatgatgt attggcaaca gctgcaaaag gtattgccga aattattact
    27841 gttcccggtt atgtgaacgt ggattttgaa gacgtaaaaa cagtattgcg tggaagtggt
    27901 cttgccatta tgggtagcgg aaaagcagca ggtcaggatc gcgcacttga agctgtacga
    27961 gttgcacttg aatcaccact gttgaacgat aatgatattc gcggtgcacg cagcattttg
    28021 ttaaatatca cctcgggaac tacgcctgtg ttgatggatg aaatagcaga gatcactgaa
    28081 tttgtgcaag aagccgcagg aaatgattgc gatattattt ggggtaactg taatgatgaa
    28141 tcattgggtg aatctattat ggtgacattg attgctacag gattcgagac tgttgaacag
    28201 cgcaatcaac gcattaaaca aaccacagtg aaggttacgc atgtgaatac acatgaatta
    28261 ctccaaaaaa aagtaacgcc gatagaagaa ccaatagcgg aagtaaagga acctgaagct
    28321 gaaccggagg ctgtgatcac attaaaacac gagcctaaag aagataaaaa agcaaagaaa
    28381 gaagaagtaa aagaaaccaa ggaggtgaag gagcaaaacc cttcgcaatt cagcttcgac
    28441 ttcggtttgt ttgccaatac tattcaggaa aaaccggtga tcaatgaagt gccgcctgtg
    28501 gttgaacctc ctgtagcaaa aattgaagaa gtttcatttg aagaaaagga ggaattagtg
    28561 atcgagttga agaagcagga cccgatcact gagattattt ctgaacagga agaggaaact
    28621 gctgaagagg aagtgatgtc gcctgtagaa atgcatcttc gtccatcagt gatcatagaa
    28681 cctgtcaacg agtttgaaga accggttgcc gaagaagaag cattggatat gccgatgatg
    28741 aatgaagtag aagaagagac gatatttgag attaaaacaa atattcgtca cgaggcaatt
    28801 gaacctgaag cggagatcga ggaagagcag atcatgattc gaaaagagga acaacgcgtt
    28861 tctcaaaaac aagagggtcg cagtttcatt accaacgatg ataaaatcaa tgtagtaaac
    28921 agcaccggca atgatcgcat caaccgtttg aaaagtatga gcatgaaatt atcgggaaat
    28981 acacttgatg aggtagaaaa agttcctgcc tttatgcgca gaaatatcga actggaagac
    29041 acgccaaacg ctcgtgaaat caatgtgtcg aaatacaata tcgttgaagg cgaagatggc
    29101 ccggagttaa aaaagaataa ctccttcttg cacgacaatg tagattgatt atacgtcata
    29161 acaaaaaaag caggccacat ggcctgtttt tttttgcccg ctccgtttat cgaaagaaat
    29221 cgccaaagtg tgtcactatc tcgctttctt gtttgttggc ataataataa tttgcactgg
    29281 aatatttgta tagctccggc gcttcaacaa gtttccaatg ttcctgtaat ggattcctat
    29341 ggatgtagtt caatttctgt ttcagaaatc cggccgtgaa tactactttt ctgtgataac
    29401 catcctgcca tattttatgc agctgctccc tttgaataaa attaatctta aaggtttcac
    29461 cggggtgatt caataacaga aaatcgcgga tcagtacact tgaatatttt ttaatatccc
    29521 gcataaaatc tgatagccgc actctgcctg aaaataggat tatcatatgc acatggtttg
    29581 gcatgattac ataccctaag agactgactt gatatatttc gctgtagcgc aataaactat
    29641 caatcaccat ttgcttagct acatcgcttt taagcaaatg aaagtggttt cggcagttga
    29701 aggtgataaa aaaacaattg gaatgttgat gttcaatcat atgctaatat gagcacatga
    29761 ataattccgt attgtaggct gaattgtgaa taatccgatc accgatgaga ctctgaaaaa
    29821 tacatttatc aattgatgtc ggcaggcaac ttaataagcg gttagtgaga aatggtgggg
    29881 atgaaaatta ctcagtttag aatgtggaaa aatcgcattt ggttttgaga ttttacatac
    29941 atggggattt cctagcctag ggtcggatgc tagcatccga ccctaggcta ggaaacgctc
    30001 aattgcaact gcagccaccg agcccttttc ttcgaaaaca aagtgtcctg catcggcata
    30061 ttgcactacc tctgcattcg ggaaaatttc ttgcatgcga tgtaaatatt tctcggtaat
    30121 gaaattgtct ttcaatcccc ataatagtaa agcaggctta ttgacaagta caccaatttg
    30181 tcgatataca ttttcgaacc acgcctgatc tttcaacagc gatttagcaa aaccgatagt
    30241 gccatttcgc gatgaacgtt tagcaaatgg tttcatatac tgtgcatgaa tttccggcgt
    30301 cagtctggtt cgttcgccaa atgctgcagg tataatataa cgcgcgctga aattgaaatt
    30361 gcgatataaa aacggcaaca gcggactgcc aagaattgcc ttcatgcgtt taaattcggg
    30421 ctcatcggca acactgaagc accatgtatt ggccatgatg attttgttga tcttttcagg
    30481 catacttgta gcaacagaaa gccctatcgg accaccaaag tcgtgcacca gtaaattaaa
    30541 ttggctgagc tgtaaatgtg caatgagtga cattaaattt tctttgtgct tatcgagtgt
    30601 atagctgtaa tcggcaggtt tatcggacaa accaaagccg atatgatcca ttgcaatgca
    30661 gcgataattt ctggaaagta tcctgatggc atcgcgaaac tcataactcc atgaaggcgt
    30721 gccgtgcaca aataagatta cctctccgga gccttcatcg atataatgca tttgagcatt
    30781 tggcagcgat aaatattttg gtgcgaaggg atattggttt gaattaagcc attcgggagt
    30841 cataattgat attttttaaa aagcgttttt tgaataagtg ataactgaaa ttgagaagga
    30901 tcgagtaggt aatgcccaac taagccatct atcagtgcga agaatattct tgcttcctgt
    30961 ttcggatgtt cgtatgataa tttgcggaag gtggcggtga gggtatgtaa tatctctttt
    31021 tgtatggcag catatcgttc accaagcaat gtgcgcaccg gttcctgcat cctgatggag
    31081 tgaaacaacc gccagtaagc attattttct gatactatgc gaaaggtatt tttaatatgt
    31141 gcccgcaagg catctttggg tgccgctccg ttttggtatt ccaatattga attggcgatg
    31201 tctgacattc cttcggccag tatcgcttcg agaagggcat cttttgatga aaaaaaatta
    31261 tacatcagtc cctgcgacac ccccgcctgt ttggcgatct gtgccaccga ggtatttcca
    31321 taaccttgtg cggcaaagag cgataaggct gttttggtga tatgtgcttt ttggttcata
    31381 tgaatgaacg ttcattcaca aaggtaaggt acatttccaa atttcaaaac cgggtgtcat
    31441 catctgtttt gcataaagac ctacctttgt atcatggcag tgcaggttta ctcggcggac
    31501 gatatcattt cccgcaccaa gcgccgacat ggcgaaacaa aaattggcga acgcattcag
    31561 accatcacta atgcagataa gtggcaggac gacctggcga catcaacagc tagttttgtg
    31621 gtgatgggaa taccggagga tatcggcgtt cgagctaatc atggcagggg aggggcatat
    31681 gcggcatgga agccgagtct cgactttttg ataaacatgc aaagcaatca ctttttagat
    31741 ggcagtgaaa tgctcgtgct cggtcacgtg atagtagatg accttatgca gcaggcagat
    31801 aagcttgatt ataagcgaga agaagatatt caaacagcgc gtcgccttgt agaacaagtg
    31861 gataatcgcg ttgaacatat tattcattca attgtagcag ccggaaaaat accaatagtt
    31921 gttggtggag gtcacaacaa tgcctatccg ctcatcaaag gaacttccaa agcatgcggc
    31981 aatcccattc acgttataaa ttgcgatccg catagtgata tgcgtccatt gcaagggcga
    32041 cattcaggaa atggattcca ttatgcctgg catgaacaat taatgtcgaa atacgccgta
    32101 ttcggattac acgaatcgta taatatttat tcggcactgg aattatttgc tcaaaatccc
    32161 gagtatctca cctttgaaac atatgacgac atattcgtac gtgagtctgt aaattttttc
    32221 gatgcattaa agcgctgcat ggattttgtg gatggcgctc catgcggtat tgagatcgat
    32281 ttggattcaa tacaaaatgt tcctgttagc gcacgaactc caagtgggat gcttcctgtt
    32341 gacgtaagaa aatttgtatt tcactgtgga gaaaatctcc cggcaaaata tctccatatc
    32401 gctgaagggg caccggttct cgcccataaa caggtagata ataaaacagg aaaactagta
    32461 gcttacctca ttacggattt tgtgaaaggg aaggttaatt agccgattag ccaattagcc
    32521 gattagccga tggaaatgtg atgatcccgc tgaggcggga tgtcgctgcg ctccatgtgc
    32581 gagtgtgcaa ttattggatt gtgtaagttt gagaacaaca gaaaaggcat cggagggcat
    32641 cctgattact attcatcaac caaatggatt attaatctaa gcacgtcaaa caaaataaac
    32701 tcatccggct gtcatcaata aggcagactc accacccgcc tatcggcgag gcaggctcac
    32761 cactcacgca ctcacccact catcacaagt gtatcacctc accatacgcc gccgccgctg
    32821 cttccataat agcttcgctc atggttgggt gcgggtgaat tgtcttaatg atctcgtgac
    32881 ctgtagtttc tagtttacgt gcagcaacaa cttccgcgat catttccgtt acgttggcac
    32941 cgataagatg tgcgccaagg aattcgccgt atttagcatc gaaaattacc ttcacaaatc
    33001 cttcaggcgt accacctgct tttgctttac cggaagctgt gaatgggaat tttccgatct
    33061 tcaattcgta tcccgcagct tttgcagcag cttcagtcat acccacactc gcaatttcag
    33121 ggaagcaata agtacatccc ggaatattac cgtaatcaat tgcttccgga tgatgtcctg
    33181 caatcgcttc aacacatgta ataccttctg cgctggcaac gtgtgctaat gccggtccgg
    33241 gagtgacatc accaatagca taatatccct cgatattggt gcgataatat ttatcaacca
    33301 cgatcttgcc tttttcagtt ttgataccta cttgttcaag accaatattt tcaatgttgg
    33361 caacaacgcc tgcagcactc aatacgatat cgcactcgag tgtctcaaca ccttttgcag
    33421 ttttcacttg tactttgcaa cctgctcctg atgtatccac agattcaaca gcagagcttg
    33481 tcatgatatt gatgccattc tttttaaact gacgtgccaa ttctttagac acttcttcat
    33541 cctcaacagg caccaaacga tccataaatt ccacgatggt cacttcagtg ccaatagaat
    33601 gatagaaata agcaaattca acaccaatgg cacctgcacc aactatcacc attttcttag
    33661 gttgaagcgg aagtgtcatt gcttcgcgat aaccaatgat ctttttgcca tcctgtttca
    33721 tagttggcag atccttactg cgcgcacctg ttgcaatgat aatatgctta gcggtgatgg
    33781 tagatttagt gccatcagcc tttgtaactt caacagattt acctgcttta acaacgccgt
    33841 ttccttcaat cacctcaatt ttattcttct tcatcaggaa ctgaacgcct ttgctcatgc
    33901 cgcctgctac gtcgcgactg cgttttacca tcgccccaaa atcggcttta gcgtcggaaa
    33961 cagtgatgcc atagtcggcc gcatgcttca tgtaatcgaa tacctgggcg cttttcagga
    34021 gggctttggt aggtatacaa ccccagttca aacagatacc tccaagctcg gcacgctcca
    34081 caattgctgt tttcaggcct aattgtgatg ctctgatggc ggttacatat cctccggggc
    34141 cactgcccaa tattattaca tcgtagttca taaaagtcga tttgcgggtg caaagatacg
    34201 gtacgtggtg ggaaaggaca atgcgaaagc catgcctccc tctaagggct gtttaggatg
    34261 aaccatattt gttgtatatt taaattatga aaacactttt aatccttcta ttggcaggta
    34321 tccaggttca ggtatttgcc caattcgaag aattttggcc catcaacagc gaaggctgga
    34381 tcactgaata taatgccaat ggtccgccac aagcaaatta tcggttttat gttgaagaag
    34441 atacactcat aggcgactat acatataagg tgctgatgcg cgataggatt aatccttata
    34501 ctgcatctac cactacgaag ttgtttgcat atttcagaga ggaggatggt gtaattacct
    34561 attacgat
//
LOCUS       DAOQOU010000012        33768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_555_length_33768_cov_5.270370, whole genome shotgun sequence.
ACCESSION   DAOQOU010000012 DAOQOU010000000
VERSION     DAOQOU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 33768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33768
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_555_length_33768_cov_5.270370"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLM90_02015"
     CDS             complement(<1..182)
                     /locus_tag="PLM90_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88170.1"
                     /translation="MLTAIPEMIATVLIVDAPSWSMWIIYTLIIVFGALVIAFMIRKR
                     MRDAAYDQRVLREKVR"
     gene            complement(163..3564)
                     /locus_tag="PLM90_02020"
     CDS             complement(163..3564)
                     /locus_tag="PLM90_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ88171.1"
                     /translation="MRNCFRKYGCIWVLLVCLNCMHVNKASGQQYFLKNYSLSDGLAG
                     ISVSSIIQDSRGYIWIGTMDGGLSRFDGKTFVNYTKHDGIGDNAITCLYEDRSGNIWI
                     GTENNGVTKFNGFEFEHFDDPGINAVDKIYSDTSGNILIYTFPDLYKIKGDSIILSSK
                     RNSEYGLQNFLLAGGPKAVKTIIDKRGNKWVASHAGISFVPKSFVDTDDQYAHAEHFI
                     LNPENPEEPASCIMQDREGNIWIGTPLSGAFMFYDGAFSNFNNLPSLRNTYITAFTTA
                     NNALIIGTPTGIKEFIYDNTADASGAQNRMYRETPVQFPGFTSTSRINALYSDPSGDW
                     IAADENGNILIHNKKPQVIRIPEIPEYAIITAISSDAENNIWVGTDRAGIFIWNNGLK
                     AHYTGDSLIISNAITALYRDAQHAIWIATMDAGVMKYENDKFTAFTYFDNGLISNNIS
                     CITQDANGMMWFGSPDEGICSFDGSGFAFYTDNDVLTSNNVNGITFDIQGNLWVALND
                     GVDHLTFNADSSITTKHFDAYDGFTGIRNNRNTIIATTDGHVWFGTVNGLFRYNPEED
                     VFSITKPVIELRNIRLYYENPDWALYSDSIGGWYNLPVNLHLPYDQNHVTFDFSAIFF
                     SVHEKIQYAVMLEGFDDTWQNIGTGTSMTYSNLKPGNYTFQVRAQNADGVWSDPVTYK
                     FSISKPFWLTWWFIVMAVVVGLSIIAILFWWRNRRFRIQAKLLEQTVMERTAELENQK
                     VMAEKAAWRAERSEKAKEEFLANMSHEIRTPMNAIMGMTRLLLEKEPKSEQLKYLKAI
                     RQSSDNLLVIINDILDLSKIQAGKMELEKIPFNVRSVLNNMAEIMKFKSDEKKLDFKV
                     IIDPAVPEFITGDQVRLNQILINLTGNAIKFTDHGHVYVYCKAVSINENTATVSFDVE
                     DTGIGIAEDKLNSIFESFSQADVATTRKFGGTGLGLSISKRLTELAGGEMRVSSTEGK
                     GSVFSVVIPYPIAEEIASSSEEDHAADIAIATNLQARILLVEDNMFNQMVATDSINDM
                     FPKAELVVADNGQIAVDKIVAEQFDVVLMDVQMPVMDGYTASRTIRALQDEQKSKIPI
                     IAMTASVIKSEVDKCYESGMDDFIAKPFDKDLLQLKIIKYAHRDS"
     gene            complement(3577..4329)
                     /locus_tag="PLM90_02025"
     CDS             complement(3577..4329)
                     /locus_tag="PLM90_02025"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HMZ88172.1"
                     /translation="MCLISFAFQSHPEFPLILIGNRDEFYARPTANLHQWENTTIPVY
                     GGRDLKEGGTWMGMNTQGSFAALTNYRDPAHMADDKPSRGNLLRDFLSGDIPLEEFHR
                     LLRSRGTAYNGFNIIYGNCEELYYYNNIQQQLRQLYPGIYGLSNAFLDTPWPKVLKAK
                     DALQRNLHISNNEDQLIHAFTDISEADDAALPSTGVSPEWEKKLSAMFITSPSYGTRL
                     TTFVSIDNRRNVVYREKGYVPEADTRFTFNIQ"
     gene            complement(4438..5319)
                     /locus_tag="PLM90_02030"
     CDS             complement(4438..5319)
                     /locus_tag="PLM90_02030"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HMZ88173.1"
                     /translation="MPIDISTPIPVIDGEKITREEFERNYVHPQKPVILRGLWKNYPA
                     YHKWNYNFFRKSMGDLEVGLYSSKQADPSQTLSTPTTKMKFSEYLKLIETEPTDLRLF
                     LFPIFKHKPELLHDFDYPPITKGYIKLPFMFFGPKNSITRMHQDIDMSNVFLTQFEGR
                     KRVVLFHPNQSDLLYKLPFNVHSTVDIEHPDYDIYPGLKYVHGMEGILEYGDTLYMPA
                     GYWHHIEYVEGGYGLSVRTLAPTLALRLRGAYNLLIQRKLDDILRFLFGKRWFELKKR
                     IAHDRANRAIEKILRDE"
     gene            complement(5410..7350)
                     /locus_tag="PLM90_02035"
     CDS             complement(5410..7350)
                     /locus_tag="PLM90_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88174.1"
                     /translation="MRSIITTILVAICLTASAQPVTEATASNKALKYLNEGVQRGMGK
                     YYDKAIESFQNAIKEEPNFITAWVYLGDTYRMLGNDSMAADTYIKALDIDPDLNSIYF
                     KRLAEAEANIGQYEEAFDHINLFLQDPDVKGSLKTEALHQKNNYNFAKEAIKSPVDFN
                     PENMGPEVNSDFPEYFPSISVDGSVLVMTRRIDDVISLGPGDARPIDNEEFFITYFRD
                     GAWTTSENMGAPINTKLNEGAQCISADGRSLFFTACDNVETGYGSCDIYYSYRIGNQW
                     TAPENCGTIINTGEWESQPSVTADGRELFFSSARFGTVGSYDIWSAKMGDDGYWEEPV
                     NLGNVINTPYSEQCPFIHPDGKTLYFSSDGHPGMGREDLFYSTRDAAGNWSKPKNLGY
                     PINTPNQEISLSVSADGKTAFFSSDRNKAPKDLDIFTFQLPAQVQADPVTWVRATVTD
                     ERTKLPLKANVQLVDIATGNTIVTSTSDPGTGEFLIVLPVGFEYGLYVQREGYLFHSE
                     NFSLLNVLPDAPFDINVALQPISQGEILTLKNIFFETASAEILPISEPELNKVVDLMK
                     KNPGMQVRVNGHTDNIGTEIDNQKLSQNRANSVKAYLVSKGIEDARISTMGFGESKPI
                     DTNANEVGRANNRRTEIEILRL"
     gene            complement(7475..10480)
                     /gene="secDF"
                     /locus_tag="PLM90_02040"
     CDS             complement(7475..10480)
                     /gene="secDF"
                     /locus_tag="PLM90_02040"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HMZ88175.1"
                     /translation="MQGKGLVKFFLAAMILVTIYQLSFTLVASRESKKADEAAQAAVD
                     PTLKGQARTEAISTAKSDYLAQNADKIVYNIGIAKYNYDDVQKRAINLGLDLQGGMSV
                     VMEVSKYDLMKQLTSDPKNQKFIKALDQARLQEAQGQGDLIELFADNYLTANPGAQLV
                     TLFANSSNVDDLPRNSDNDAVISWLKKSADDGVESTYTKLKERIDKFGVTQPYVTLQK
                     STGRIMVELPGVDNPKRARELLQSTANLQFWGVWRYEEVGGAINQANNVLTQLEDIEA
                     PVSGDTGVTENTSPVDSPAVAATTPAGDDSLAANDDTLLADNEEAQTEDLGPLSSLLL
                     ERSDPAAIGMVAEDDTFKLMNYFRMEEVANVFPEGTKFMLSAKPAKGSRNYDVYAIKS
                     NGDDDQPLLDGSVVKNASPDRDQVQNIVISLSMNTEGAQIWKKVTEANVGNFIAIALD
                     NKVYSAPRVNEAIPSGRSSISGSFTAEEAQDLSNILEVGKLPVPSRIVQEESVGPSLG
                     AESIRVGLLSMIAGLILVIIFMVIYYSTAGMIADIALFANIFFIFGALASMGATLTLP
                     GIAGLVLTMGMAVDANVIIYERIREELDKGKSMIKAVADGYSGSYSAIIDGNLTTLII
                     AIILMNFGTGPVKGFAVVLTIGIIASLFSSILISRLIIDAMVKKDRPVKYYTSFTKHA
                     FKNVKWNLMGNRRKGYLFSGALTIIGVIVMFTSGFNFGVDFKGGRTYTVRFEESIDAD
                     KVRNELADFDPGVQVKTFSSSDKLKITTGYMIENPDPGTDTIVAQELYKQLAGLYHNV
                     DYKTWDSDFKESQMKVEPTIATDIQTSALKAIGLALIAIFIYIVFRFRRWQFGVGAIA
                     SLVHDAFMVLAFFSILKNVMPFSLEINETFIAAILTVIGYSINDTVVVFDRIREYLRE
                     SKAGSVQQIFNTAINNTLSRTVITSGTTLLSILVLLIFGNENIQGFAFALFVGIGFGT
                     YSSIFVASAIALDMYKKEDHHKSAEEIMN"
     gene            complement(10685..13285)
                     /locus_tag="PLM90_02045"
     CDS             complement(10685..13285)
                     /locus_tag="PLM90_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HMZ88176.1"
                     /translation="MIKQVTPLLMGMLCICGVANAQLNTELLGHLPYADQLSNLTGWS
                     DGAGNEYAIVGTYDGTSIVDITDPTDPTEVAFVNGNNSIWREVRTWDHYAYVVTEAGG
                     GLLCIDLSGLPASVSYNYTNCGIGLSTGHTVFCDENGFVHIFGSNIGAGGDQILDANA
                     NPMDPEFAGEVDMWYIHDGYVRGDTLWAGNIYNGWFSVWDISDKTSPVLKAQQSTPDN
                     FTHNTWLTDDGNYLFTTDEVTNATVASYDVSDLTDIKELDQFQANPGTNSIPHNVMVL
                     GDFLVTAYYRDGVVITDATYPDNMIKVGEYDTSPFAGDGFNGAWGSWPYLPSGNIIVS
                     DMEEGLFIVGPTYVKACYVEGNVTDASTGAPIFGATVNFTGVVDATSDLIGDYHGGVL
                     DAGTYDFTFNKTGYLPETVTGVVMENGIVAIVDAELTPLPSYTITGQVLDAATGAGIA
                     GADVLFQNALYNFSGTTDASGNFSFDMYEDDYDFIAGDWGYVTNGINVTVSAGGTYTI
                     TLSKGYYDDFIFANDWTTSGTTDVGMWERGEPNGTFAGPDASNPDFDVTDDYGDQAYV
                     TGNEPGGTVGTDDVDNGIVNLISPSMDLTGYADPVLSYDRWFFNGGGTGTPNDTLRIF
                     VSNGIEEVLIDAINSSSTELSEWVNVEVNLSTYIDITSDMHVRFRTADQSASGHLVEA
                     GVDLFMVYDNITSEPTTAFGSDVTTGCGPLTVVFDDMSVGATSWSWTFPGGTPATSTL
                     QNPTVVYATPGTYDVTLVASNIVGESESTTTGYVTVNLCNAIDEVSPAGFTVSPNPFN
                     TLFSVEIEAANATDIRIIDITGRIIRTLQTNGEQVMVIAADQLPAGIYTIELLKNNEV
                     IGTTKAVKAE"
     gene            complement(13543..13746)
                     /locus_tag="PLM90_02050"
     CDS             complement(13543..13746)
                     /locus_tag="PLM90_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMZ88177.1"
                     /translation="MKKNMGSTDRIIRVILAIVFALLYFTNTVTGTLGLVLVILGGVF
                     LLTALVRFCPLYAPFGISTCKTK"
     gene            complement(13910..14536)
                     /locus_tag="PLM90_02055"
     CDS             complement(13910..14536)
                     /locus_tag="PLM90_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ88178.1"
                     /translation="MMDVLSLQQKLPLLEEALIREIIERAQEVTIPPQQQILREGQYI
                     KTIPVVMNGLLKVITGSGDKDMLLYYIKPGDSCIMSFAFSLSNEPSNIAAFTETETTM
                     LLLAVDDVRQWLIAYPSLNMLFLNLYNHRYRDLIDTIGLIYEKMDNRIMHYLEQKVKI
                     TGNNIVNITHREIAADLASSREVISRLLKKLELEGKLQQTPAGIKILV"
     gene            complement(14533..15294)
                     /locus_tag="PLM90_02060"
     CDS             complement(14533..15294)
                     /locus_tag="PLM90_02060"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HMZ88179.1"
                     /translation="MTEKQLIAYRKEVENCIHKIEPSKSSFKGFDLQHYIGTAFTVSG
                     VKMPVARKLSRNGFSFSHLEADEQYIIWKYIWHTTDNFDTMTQAIFFCDKYIRLANPE
                     FFFNEMCEWLQRVDNWAHSDSLTHYFAALMESHPAMVYPVLQKWNKSDFSWERRQSVL
                     SLLDYARFRKKYPTFNKIIALVKPLLKDKDVFVQKGVGWCLRETGIIYPTETYAFLVD
                     NHAAISSIAFSAAAEKLSPAKKEKLKSLRKKSRKS"
     gene            15390..16535
                     /locus_tag="PLM90_02065"
     CDS             15390..16535
                     /locus_tag="PLM90_02065"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HMZ88180.1"
                     /translation="MQKRITQLLCGVLLTTTPLLSNAQLTLPPSGDNQQSAVSQWMGP
                     VRVDITYSSPDVHAPDGSDRTGHIWGELVPYGMVNLQFGYSNENHPSPWRGGANMNTT
                     ISFSHDVLVNGSALKAGTYGLHFIPNKDEWTVIFSSNSTSWGSFFYKPEEDVLRVQTK
                     PAANNYHEWLTYEFTDRQTDACTVELMWELLRVPISIKVPDIKQVYVENFRKQLRSDV
                     GFNYQAWVDAANYCVANNVNLQEALTWANYAIEEPFFGRVNFATLSCKASVLKALGKT
                     VQSDSIMKVAIADPTASLMDVHFYARGLQAEKKNKEALEIFLINYQKHPESPVTNLGL
                     ARGYSANGDYKNALKYAKAAQKLNPDPQVKQTLENAIKTLEAGKDFN"
     gene            complement(16605..18218)
                     /locus_tag="PLM90_02070"
     CDS             complement(16605..18218)
                     /locus_tag="PLM90_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ88181.1"
                     /translation="MLSLLLAIFFVGNAFGQSTATTTETEAPKLTEAEALEKFDRFLS
                     IVDDRYMDDVDAGKLVEDAIIEVLAQLDPHSVYFTEDELKEANEPLEGNFEGVGIQFN
                     LLRDTIFVVSVISGGPSEKVGILAGDKIVQINEENVAGIGMSNKGVTDRLRGKKGTDV
                     RVYIERKGAKKPLEFKITRDVIPLYSIDAKYMASPETGYIRVSKFAATTTEEFQAAVK
                     ELKSSGMKNLILDLRGNGGGYLNAAIDMANEFLPANKLIVYTAGRAYPRQDALSRGNG
                     MLQDGKVVVLIDEGSASASEIVTGALQDWDRALVIGRRSFGKGLVQRSFSLTDGSAMR
                     LTVSRYYTPSGRCIQKSYEDGIDAYHNELNERYRDGELTADTTFHYPDSLTFYTSGKR
                     VVYGGGGIMPDIFIKLDTSWATDYYAEVVGKGIVNQFALTYVNANREKLNKSYSDVYA
                     FSNKFNVDEALWNEFFTYAEKDSAKKDDSTFSLCEAKLANVIKSVIARDLYDFQAYWI
                     VSNSDDEAYLRALEALKDDTFEKMKIAGK"
     gene            18465..19328
                     /locus_tag="PLM90_02075"
     CDS             18465..19328
                     /locus_tag="PLM90_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ88182.1"
                     /translation="MVLSTFLFAVMNVIVKYLHRLPSHELVLFRSLVMFVITAGMLYR
                     AKLQPFRYQPRWILIGRGVAGSLALLIYFFTIQHLPLASAVTLGYLSPIFTIIFASFL
                     LKEGMRWVQWVFFLVSFAGVVIMHGFESAEDTPLVLLGVLGAAFSGLAYNALRKSAGT
                     VPTLVVVFFLPLCTIPLVIPFCLHNWIMPVGYEWLLIIAMGAVTQAAQIYMTKAYQLA
                     KAGAIANYAYLGVVYALILGYFIFDERFAFIGVIGIGIVILGIILNYFYVNRVTSLKR
                     FVVYFRWFPGL"
     gene            complement(19346..20515)
                     /locus_tag="PLM90_02080"
     CDS             complement(19346..20515)
                     /locus_tag="PLM90_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HMZ88183.1"
                     /translation="MEQDRYALRGVSSQKEEVHKAIKNMDKGLFPNAFCKILPDLIAG
                     DPAFCNVMHADTAGTKTSLAYIYWKETGDLSVWEGIAQDALVMNLDDMACVGITDNII
                     ISSTIGRNKSIIPADVIAAVINGTNNFLANMQLHGVQIHHAGGETADVGDIVRTIDVG
                     ITAFGRIDRSKLIVNDIKPGQVIVGLASYGKSIYETAYNGGMGSNGLTSARHDVFHHQ
                     YADKYPESFSPEIPREVVYSGSKSLTDEITVNNEKITLGKLVLSPTRTYLPVIRTMLE
                     NHSDAIKGMIHCSGGGQTKVLHFADKVHIVKDNLFATPPLFDIIQQESGTSWKEMYRT
                     FNMGCRLEIYTDQNTAESLINIAKSFDIEAQIIGHVMPSENKKLTVKSAYGEFEY"
     gene            20660..22771
                     /gene="ppk1"
                     /locus_tag="PLM90_02085"
     CDS             20660..22771
                     /gene="ppk1"
                     /locus_tag="PLM90_02085"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HMZ88184.1"
                     /translation="MAGKKQKKEKPKFINRDLSWLSFNERVLQEAEDKRVPLNDRIRF
                     LGIFSNNQDELFRVRVAVLRRMMRLQRGDLKQVDMNPSKVLNQIQKKVLVLKNRFDNA
                     YLQILEELKKHKIYILNEKEISTQQGEIIKNYFNQHVRQWLFPIMIDESKKMPLLNDK
                     SIYLAIRMVKKDKPLATRYSLIEVPDGDLPRFFVLPDDKEKKFVMLLDDIIRYNLDSI
                     YYIFNYDSIEAYTIKITRDAELDISTDLSQSLLEIIQKSVKRRNKGETTRFIYDAQMP
                     EEMLQFFARKMKLQRQDPIIPGARYHNFKDFMKFPDLGRDVLTYPELVPHHHPLLNTY
                     SSITSVLRKRDVLLHFPYQSFDHIIDLLREAAINPNVTTIKMTLYRVARHSSVVMSLI
                     NAARNGKQVTVVMELQARFDEENNIFWANRLQEEGVRVLFGSNNLKVHSKLCLINHHH
                     EGKDVQYAIVGTGNFNENTAKVYTDLFLLTAKPEVTKEVKKIFDIFENKFELGKYKHL
                     LVAPINLRSRLKLLINREIKFAKEKKKAAIFLKVNNLVDKEIIKKLYEASMAGVQIKL
                     IVRGACSIVPGVEGLSDNIEAISVLDRFLEHPRVFAFHNNGKEEVFIGSADIMERNLD
                     HRIEVMVQVLDKDIRKMIKDILTIQWGDNEKARLLGHGHLNDYVKVLPGEKQVRSQMA
                     LYDYFEKSRLASALEKKEGNK"
     gene            22774..23670
                     /locus_tag="PLM90_02090"
     CDS             22774..23670
                     /locus_tag="PLM90_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HMZ88185.1"
                     /translation="MKFAAIDIGSNAVRLLIENVYETSSGPVFHKDSLIRIPVRLGED
                     AFLDGAISEEKVQMLVKAMVAMRHIIDIHKVTAIKVAATSAMREAANSGDIINRVMEA
                     SGIQIEVITGDSEGKAIMQLPLEQIGLNPALHYLYIDVGGGSTELAFIGKGDLTANRS
                     FNIGTLRLLNDQVSDADWKEIPDWIKAHKHHGFDLHAIGVGGNINHVMKHYGKAGKPY
                     ISMAILKRVMMYIESLSMGERVRTLGLKPDRADVIVPALKIYINVMKWAGIEKIYVPK
                     QGLSDGLISQLYHEWKSKVVTP"
     gene            complement(23660..24211)
                     /locus_tag="PLM90_02095"
     CDS             complement(23660..24211)
                     /locus_tag="PLM90_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88186.1"
                     /translation="MTRSDNYVSPTIFTDRLILRNPSLNDAFNMFLQRTDAEVNQFVP
                     MTPPKDPSEVETLLRTIIHNTSTGISDYWIITDKQNTYLGSICLWNYNYESYTAELGF
                     VLIKNAWNHGYATEAIERVITFAMEKLPFTIIEGWTHHQNFTAQHAMQKNGFLRNMEA
                     EKNIDQSSPDADMQIWTLKLIRE"
     gene            complement(24208..24810)
                     /locus_tag="PLM90_02100"
     CDS             complement(24208..24810)
                     /locus_tag="PLM90_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ88187.1"
                     /translation="MPTLYKNIKICIGKSPTAWRNWLEKNHAKEEKVWLVMFKKNSGI
                     PSLTWEEAVDEAICFGWIDSVRNSRDENSFYQYFAKRKPKSGWSDINKKKVERLLNEG
                     KIAPAGLEMIQLAKSTGTWDTLNEVETLAIPPDLQSALKKNKTALKHFNAFPASTKRA
                     ILQWLQSAKKEETRKRRIAQTVAEASQNIRTTQTNQPKLK"
     gene            complement(25161..30023)
                     /locus_tag="PLM90_02105"
     CDS             complement(25161..30023)
                     /locus_tag="PLM90_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMZ88188.1"
                     /translation="MKTFTLTTALAIITNLTFAQLPDQDYILDFIDPSITRSASGDIN
                     NDGIIDYVGGYYQIHWFEGNGTYEFGTIHNTGAKLNEYLTDLKVVDIDHDGWKDIWYQ
                     KGTVPFEMGWLKNPGATDQEWTTFSVAGSYRVCLGIFDMNGDGNEDFVFEDGAYLLGY
                     RELGDGNFSVYHAIPDSWSWVESFSAEDLDGDNDVDLAVYCYDSGVKYWRNNSNGTFT
                     STNLKNSESSYDVYSGDVNGDNHPDILYNNGSFYVSTFDTITHTFSPGVYNGNMDDFD
                     FRKFLNIDLDLDGDKDFVIVNDNYFAGESTYEISWLESDGSFLNLNVENFESIIAGLP
                     YYNSCMSFNDMNNDGTFDFIQCESGDYGTTAIVSPNIGGFFGAYDSSFLEVSPEVADL
                     IVYDVDGDPDNDLLLAFNNGLLSWFEFNHLVDSFTIMHNINYTGSRPTPENIKKKDID
                     SDGDFDIISSFSYNGALARTFYHLDLGDGNFETHTLCDTAFSNVWLIDGDEDGDFDLA
                     GLKAGDLNRLLYFTNTGNPDHLFSYTTELTAYGYYNSFVQLDIDDDGDDDLVGIRNDA
                     NGIKKIINLDGGLLFEDISNLFVSACDDVKTLFTSDFDNDGDKDFAYVCGDGTMHMAL
                     DNGTGYTDSAIGTFDPATLSDGSHTAMFDMNMDGLEDFVFNKTSGGSGFKLATGPGTF
                     QEDYDLFSTDYGRFANVDPGTLPDFVGISSNGLYIQYNILMLPPSFEVTPPTQTYISE
                     GAEEDSIIITFNTIPQEPLEINVLPYGILNAGAGESIGYTFIVDADSTALEPKVIHLS
                     TNEDAIAEDITIGGVTITADEVSWGAFASLLDEHYDFTITDNDLAIFTTNDALEINEG
                     IWSDTVFAHLNVIPQNDVLIQAIADPNYISAFGNDMTETYTIPSGIVGIAPFKMTLLY
                     PEDATYFGDIASSLTIELLSDDAAIDAFTSELLPVTLIENDVVDVQVVFGTTELSEGI
                     ESTTMTVSLTSAPTQDVTITYTPDIYLDLGLGTGVPVSITFSGGDVIPSAQTLTVTAV
                     DNFFAEGTHDAMIIESIVSADIYYADITPASYIFSISDNDLPQGITVTLPPAATYMEG
                     TTDILMSMSLWTNPNALVTIIGHPDANIDLGLGAGIDNSISFDPLDDATIPRIFNITA
                     VNNTIIGPEITLANISFEIITTDPVLDDEIIEPAEISIQDEDMLQGIQIAAPPAIAYT
                     EGTADLTMSLMLNVEPNSAVTINCIPDAQLDFGAGAGVAVSVLLNDLDATLPHEFSID
                     IVDDEIIEGFHEGNITFEIITDDPVLTGVTIDPLVISIDDNDFPQSVIISVDGFTHTE
                     GEAGIPFSVALESVPDLDADILLSPSEDIDLGAGFGAIVNLHFENDETAMTPIILTIA
                     VKDNFIVDGDHIGSILAELITDDPFLSDYLIPDITINILDNDAEQGVNITTPGTTFYA
                     EGTTGLSVSLSLFTIPNDIVTISATYDDTQIDLGLIESLTFATEATAMEMQYFNVDII
                     DDAIVEGDHYALINYIITTDDPQFDGYTIPFTMINISDNDVESGISNATTDNLPTVVV
                     TSGQFQINMPDSYNGKDFAITSINGQIVSKGIIDGNLLHGDLSRMSAGRYMIAIVDGE
                     KMLSIPVILN"
     gene            30351..32735
                     /locus_tag="PLM90_02110"
     CDS             30351..32735
                     /locus_tag="PLM90_02110"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMZ88189.1"
                     /translation="MKKLLLGCLAFTGVLTAKSQVTPITVSGEITTNTTWTKNNIYLL
                     SGFVYVEDGATLTIEPGTLIKGDKDSKGTLIVTRTGMINAAGTACEPIVFTSNQPAGS
                     RTYGDWGGVIILGEAPINVPGGTAVIEGGVDTPEGDGVYGGALPADNSGTFTYVRIEF
                     PGIPFLPGNEINGLTMGGVGSGTTINHIQVSYSGDDSFEWFGGRVNSKYLIAYRGWDD
                     DFDTDYGYRGKGQFFYALRDPNIADVSGSNGQEQDNDATGSANTPTSRPLFSNITIAG
                     PKVNTGDVVNVNYKRGAHDRRNTMASTYNSIIMGYPECGLKIEGVNTSLNAQNDSLQW
                     KKNILSGHGDDYICTTCDVGFNIDTWASTNGNATYATNALVGLVDPFNLSNPDPRPTA
                     GSAPTTNGTYFSGLLADPFFTATSYAGAFSTTNNWTDESWVNFDCQNTPYNTPGISNN
                     PVVSAVVTSMQCANTGAIDVSVSGGIGSYTYLWNDGATTQDRTGLNAGSYTVTVTSGT
                     CTTTATYTVSDITITKPTSLTTTQATHCSIKLNWAAVPGAGSYEVRYRITGGTYSSPV
                     NVGAVTSYTFTGLAASTSYDLQVRAKCPTGAEKSAYAKKTSSTVACGAPTSQSAGSIT
                     ANSAVISWSIPCSVTNYNLQYRKNPTKAWTTVSGIATTSYTLTGLLPSSTYEYRVASN
                     CGGTLSAYTGFSNFVTLPFRLEGEVEMPAIDADMEIYPNPAVDEVNMVINAQGVVNVI
                     ITNMLGQVVYNLENVEVNGTETVSLSLEGFDTGIYHVTIQNDVIKLTDQLVVSK"
     gene            32878..>33768
                     /locus_tag="PLM90_02115"
     CDS             32878..>33768
                     /locus_tag="PLM90_02115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ88190.1"
                     /translation="MQRLIFLAFLLITATIAGKAQNGMISGVIIDGKTAETLIGANVL
                     IKEKAGTGSSTDIDGRYKIDDLEPGTYTVEISYISYDTKTVPGVEVKPGQVTSLDVTL
                     TSGLMELTVVEVKARPRQESLNTVLAFQQKNIAPSEVISRDIITSTPDKNTGEVLKRM
                     SGTTITDGKFVVIRGLSDRYNLALVNGNLLPSTEPDRKTFAFDLFPSAMLDNLIVIKA
                     AQPNLPGDFAGGIIILNTRDIPDKPFISISAGAGYNTISTFKEYYTYTGGKTDWLGID
                     DGTRQLPAGFPVSQDLFDDLT"
BASE COUNT         9463 a         6722 c         7420 g        10163 t
ORIGIN      
        1 tcgcgcactt tttcgcgcag cacacgctga tcgtatgcgg catcgcgcat gcgttttcgg
       61 atcatgaagg caattaccaa tgcaccgaaa acgatgatca gtgtatagat gatccacatc
      121 gaccatgatg gagcgtcaac gatgagtacc gttgctatca tttcaggaat cgcggtgagc
      181 atatttaatt atttttagtt ggaggaggtc tttatcaaat ggtttggcaa tgaaatcatc
      241 catgccgctt tcataacact tatctacttc acttttaatt actgatgcgg tcatggcgat
      301 aatggggatt ttgctttttt gttcatcttg caaggcgcga atggttctgg acgcagtata
      361 tccatccatc acaggcattt gtacatccat aagcaccaca tcaaattgtt cagccacaat
      421 tttatcgacg gcaatttgac cattatcggc aacaacgagt tccgctttcg ggaacatgtc
      481 gttgatgctg tcggtagcta ccatctggtt aaacatattg tcttccacca acaaaatgcg
      541 cgcttgtaaa tttgtggcga tggcaatatc ggcagcatga tcttcttcac tgctacttgc
      601 aatctcttct gctatcggat atggtattac tacactgaac actgagcctt ttccttcagt
      661 gcttgaaaca cgcatttcgc caccggcaag ttctgtgagg cgtttgctga tacttaatcc
      721 caatccggtt ccaccaaatt tgcgcgtggt agctacatca gcctgcgaga atgactcgaa
      781 tatactgttt aatttatctt ctgctatgcc tattcccgta tcttctacat caaaggaaac
      841 agtagctgta ttttcattta tggaaacagc tttgcaatac acatatacat gtccatggtc
      901 ggtgaattta atggcatttc ctgtcagatt tatcaatatt tgatttaatc gcacctgatc
      961 gccggtaata aattcgggaa ctgcagggtc aatgattact ttgaagtcta atttcttttc
     1021 gtctgattta aatttcatga tctcggccat attattgagc actgaccgta cattaaacgg
     1081 aattttttcc aattccatct ttcctgcctg aatttttgat aagtcgagaa tatcattgat
     1141 gatcaccaac agattatcag aagattggcg aatagctttt agatatttta gttgttccga
     1201 tttaggttct ttttcgagca gcagtcgcgt catacccata atggcattca taggtgtgcg
     1261 gatctcatga ctcatgttgg cgaggaattc ttcttttgct ttttcagatc tttcagcacg
     1321 ccaggcggct ttttcagcca tcactttttg attttccaat tcggcggtgc gttccattac
     1381 agtctgctcc aataattttg cctgaatgcg gaatcttctg tttcgccacc agaataaaat
     1441 ggcgatgatg ctcaatccaa ctacaacagc catcacaata aaccaccatg taagccagaa
     1501 tggtttgctg atagaaaatt tatatgtgac aggatcactc catacgccgt cagcattttg
     1561 tgcacgtacc tgaaatgtat aattgcccgg tttgaggttt gaataagtca tggaagttcc
     1621 ggtaccgata ttctgccatg tgtcgtcgaa gccttcgagc attacggcat actgaatttt
     1681 ttcatgcacc gaaaagaaaa tggcactaaa atcgaaggtg acatgatttt gatcgtaggg
     1741 gaggtgtaga tttaccggca aattatacca accgcctatt gaatcgctgt atagggccca
     1801 atccggattt tcataataca atctgatatt gcgcaattca atcacaggct tggtaatact
     1861 gaaaacgtct tcctccggat tatagcgaaa taatccattc acagtaccga accatacatg
     1921 gccatcggta gtagcaatga tggtattcct attatttctg atgcctgtaa agccgtcata
     1981 ggcatcgaaa tgttttgtgg tgatactaga atcggcattg aacgtcaggt gatctacacc
     2041 gtcatttaat gcaacccata aatttccttg tatgtcgaat gtaatgccat ttacattatt
     2101 ggaggtaagc acatcattgt cggtataaaa tgcaaaaccg ctaccatcaa aactgcaaat
     2161 accttcatca ggacttccga accacatcat tccgtttgca tcctgcgtta tgcatgaaat
     2221 attattgctg atgaggccat tatcaaaata ggtaaatgcc gtgaatttgt cattttcata
     2281 tttcatcaca ccggcatcca tagttgctat ccagatagca tgttgtgcat cgcgataaag
     2341 tgcggtgata gcatttgaga ttattaagct gtcgccggta taatgtgctt ttaatccgtt
     2401 attccagata aaaattcctg ctctgtcggt gcctacccaa atattatttt ctgcgtcaga
     2461 agagattgca gtaataatgg catattcagg aatttcagga atacgaatta cctgcggttt
     2521 tttattgtgg atgagaatat ttccattttc atctgccgca atccaatcgc ctgatggatc
     2581 gctatagagg gcgttgatgc gagatgtaga agtgaacccg ggaaattgaa caggcgtttc
     2641 gcgatacatg cgattttgcg ccccggaggc atcggcagta ttatcgtaaa taaattcttt
     2701 tattcctgtt ggtgtgccaa tgatcaaggc attgttggca gtagtgaatg cagtgatgta
     2761 tgtattgcga agtgaaggaa gattattgaa atttgaaaat gcaccatcgt aaaacataaa
     2821 tgcaccgctc aaaggagtgc ctatccaaat attcccttca cgatcctgca tgatacatga
     2881 tgccggttct tcaggatttt caggattgag gataaaatgt tctgcatgtg catattgatc
     2941 atctgtatca acaaatgatt tcggcacaaa ggaaatgcct gcgtggcttg ctacccattt
     3001 attgcctcgt ttgtctataa ttgtttttac ggctttaggt ccgcccgcta gcaggaaatt
     3061 ttgcagtccg tattcgctat tgcgtttgct tgaaaggata atactgtcgc ctttaatctt
     3121 gtagagatca gggaacgtat aaatgaggat attaccggag gtgtcgctat agattttgtc
     3181 gacagcgtta ataccgggat catcgaagtg ttcgaattca aaaccattga attttgtgac
     3241 gccattattt tctgttccta tccatatatt cccgctgcgg tcttcgtaca ggcaggtgat
     3301 ggcattatcg ccgataccat cgtgtttggt atagttaaca aacgtttttc cgtcgaagcg
     3361 ggataatccg ccgtccatgg ttccaatcca gatatatcct cttgaatcct ggatgatact
     3421 tgaaacggaa atccctgcta aaccatcgct caggctataa tttttaagga aatactgctg
     3481 gccggatgct ttgtttacat gcatgcagtt gaggcatacc aacaacaccc agatacagcc
     3541 atatttccgg aaacaattgc gcatgtagca gatatcttat tgaatattaa aggtgaaacg
     3601 tgtatcagct tcaggaacgt atcctttttc gcgatatacc acatttcgcc ggttatcgat
     3661 acttacaaat gtagttaatc gggtaccgta ggagggcgaa gttatgaaca tggccgacag
     3721 ttttttctcc cattcagggc ttactcccgt tgagggtagg gcagcatcat cggcttctga
     3781 aatgtcggta aatgcatgta tcaattgatc ttcgttattg gatatatgca gattcctttg
     3841 cagggcgtcc ttagccttga gcacttttgg ccatggagta tcaagaaagg cgttgctcaa
     3901 accataaata ccgggatata attgacgcaa ttgttgttgg atattattgt aataatataa
     3961 ctcttcgcaa ttgccgtaga tgatattaaa accgttgtaa gctgtccctc tgcttcgcaa
     4021 tagtcgatga aattcctcca atggtatatc gccgctcaaa aaatcacgca aaaggttgcc
     4081 gcgcgaaggc ttatcgtcgg ccatgtgagc gggatcgcga tagttggtaa gcgctgcaaa
     4141 tgagccttga gtattcatgc ccatccaggt gcctccttcc ttcaaatcgc gaccgccata
     4201 aacgggaatt gtggtatttt cccactggtg taaattcgca gtagggcgag cataaaattc
     4261 atctctgttg ccgattaaaa taagcggaaa ttcaggatgc gattggaagg caaaactgat
     4321 cagacacatg tcatgaagat agtgttttat gttgcatcaa catatcttca ggacggaatc
     4381 gttcacatcg cctaattatc atcaatacaa tgccggattg tgttgattgt gattttccta
     4441 ttcatcccgc aatatttttt caatggcacg gtttgcacga tcgtgggcta tacgcttttt
     4501 caattcgaac caacgttttc caaaaaggaa acgaaggatg tcgtcgagtt tgcgttgtat
     4561 caacaaatta tatgcaccgc gcaatcgaag ggcaagagtg ggggctaagg tgcgaacact
     4621 caatccatat ccgccttcaa catattcgat atgatgccaa taaccggcag gcatgtataa
     4681 tgtatcgcca tattcaagta tgccttccat gccatgcaca tattttaatc ccggataaat
     4741 atcataatca ggatgttcga tatctactgt agaatgcaca ttgaaaggga gcttgtacaa
     4801 caggtctgac tgattgggat gaaatagcac cacgcgtttt cttccttcaa attgtgtaag
     4861 aaacacattg ctcatatcga tgtcctgatg catgcgtgtg atgctgtttt tcggaccaaa
     4921 gaacatgaat ggtaatttga tatatccttt agtgattgga ggatagtcaa agtcgtgaag
     4981 taactcgggt ttgtgtttga aaatagggaa gaggaataag cgcaaatcgg taggctccgt
     5041 ttcaatgagt ttcaggtatt cgctgaattt catttttgtt gtcggcgttg aaagtgtttg
     5101 gctcggatca gcttgtttgc tgctgtagag gccgacttcc aaatcgccca tggattttct
     5161 aaagaagttg tagttccatt tgtggtaagc cggataattt ttccagagtc ctcttaagat
     5221 taccggtttt tgggggtgaa catagttgcg ttcgaattct tcacgagtga tcttttcacc
     5281 atcgatcacc ggaattggtg ttgagatatc gatcggcata tagcaaggtt ttatagtgta
     5341 tcattaacag tgaatttaaa tcctttgttt catcgccggt gttaaattca tttaaattac
     5401 acgtgcacct cacaagcgca ggatttcaat ttcagtgcgt cggttattag cgcgacctac
     5461 ttcgttggca ttggtatcga tgggtttaga ttcaccaaaa cccatggtgc tgatacgtgc
     5521 gtcttcaata cccttgctta cgaggtatgc tttcacactg tttgccctgt tctgtgataa
     5581 tttttgatta tcaatttccg taccgatatt atctgtatgt ccatttacac gaacctgcat
     5641 accgggattt ttcttcatca gatcaaccac tttattcagc tcaggctcag atatagggag
     5701 gatttcagcg gatgccgttt cgaagaaaat atttttcagg gtgagaatct ctccctgact
     5761 gataggttgt aacgccacat taatatcgaa tggggcatcg ggcaacacat tgaggaggct
     5821 aaagttttcg gagtggaata aatacccttc tcgttgcaca tacaggccat attcgaatcc
     5881 gacaggtaac acgataagga attcaccggt gccgggatcg gaggtagagg taacaatagt
     5941 atttccggta gcgatgtcaa ccaattgcac attagctttg agcggtaatt tggtgcgttc
     6001 atctgtcacc gttgcgcgga cccatgttac tggatctgcc tgtacctgcg cagggagctg
     6061 gaatgtgaag atatcaaggt cttttggtgc cttattccta tcgcttgaga aaaatgccgt
     6121 cttgccatcg gcagatacac ttaggctgat ttcttgattc ggggtattaa tcgggtagcc
     6181 gagattttta ggtttcgacc agtttcctgc tgcgtcacgt gtggagtaga agaggtcttc
     6241 tctgcccatg cccgggtgac cgtcgctcga gaaatacaat gttttgccgt cggggtgaat
     6301 aaacgggcat tgttctgagt atggggtatt gattacattt ccaaggttca ccggttcttc
     6361 ccaatagccg tcatcaccca tttttgccga ccagatatcg tagcttccaa cggtgccaaa
     6421 gcgtgccgag ctgaagaaca attcgcgacc atcggctgtc actgaaggtt gagattccca
     6481 ttcaccggta ttgatgatag tgccgcagtt ctcaggtgca gtccattgat tgccaatgcg
     6541 atatgaatag taaatgtcgc agctaccata accggtttct acattatcgc aagctgtaaa
     6601 aaacagggat ctaccgtctg cagagataca ttgtgcgcct tcatttagct tggtattgat
     6661 gggcgctccc atattttccg aggtagtcca tgcgccatca cggaaataag tgataaagaa
     6721 ttcttcgtta tcgataggtc tggcatctcc gggtccgagg ctgatcacgt catcaatacg
     6781 ccgggtcatc acgagcacgc tgccatcaac agaaatggat gggaagtatt caggaaaatc
     6841 agagtttacc tccgggccca tgttttcagg gttgaagtcg acaggagatt tgatcgcttc
     6901 cttagcaaag ttgtaattgt ttttctgatg taatgcctcg gttttgagtg agcctttcac
     6961 atcaggatct tgcaggaata gattgatatg atcgaatgct tcctcatatt ggccgatatt
     7021 agcttcagcc tctgccagcc gtttgaaata gatgctattc agatcaggat cgatatccag
     7081 tgctttgata taggtatcag ccgccatgga atcatttcct aacatacggt aggtatcgcc
     7141 aaggtaaacc caagccgtga tgaagtttgg ctcttctttg atagcgtttt gaaagctctc
     7201 gatcgcttta tcgtaatatt tgcccattcc tctttgaacg ccttcgttaa ggtatttcag
     7261 agccttattg gaggctgtag cctcagtaac aggctgtgca cttgcagtaa ggcatatagc
     7321 cactagtatg gtggtgatga tagatctcat tacagtatta taaacgtaaa gatgcgtgtt
     7381 tactgatatg taaacaaaaa ttgttccgca catgcataca aaagaaaaac ccctgtggga
     7441 agcaggggtt tttcgcttag tcagagtggt gtgcttaatt cattatttct tcagcagatt
     7501 tgtgatgatc ctctttttta tacatatcta atgcgattgc agaagctacg aagatagatg
     7561 aataggtacc aaaaccgata cccacaaaca atgcgaaggc aaaaccttga atgttttcgt
     7621 ttccaaatat caataatact aagatagaca ataaagttgt acctgaggta attacggtac
     7681 gacttaatgt attatttatc gcagtattga aaatttgttg cactgaaccg gccttacttt
     7741 ctctcagata ctcacgaata cggtcgaata ccaccaccgt atcgttgatg gagtaaccaa
     7801 tcaccgtcag aattgctgcg atgaacgttt catttatttc aagtgagaac ggcattacat
     7861 ttttaaggat ggagaagaat gccagcacca taaatgcatc atgcaccagc gatgcgatag
     7921 caccaacacc aaattgccat ctgcggaatc ggaatacgat ataaataaag atagcgatga
     7981 gtgccaaccc gattgctttc aaggcagatg tctgaatatc tgtcgcaatg gtaggttcaa
     8041 ctttcatttg gctttctttg aaatcgctat cccaggtttt ataatcaacg ttgtgatata
     8101 atccagccaa ttgtttgtat aattcttgag caacaatagt atcagttccc ggatcaggat
     8161 tttctatcat ataaccggtt gtgattttca gtttatcgga gctactgaaa gttttcactt
     8221 gtacacccgg atcgaaatca gcgagttcat tgcgcacttt atcagcatcg attgattcct
     8281 cgaaacgcac tgtatatgtg cgaccgcctt tgaaatctac accaaaatta aatcctgagg
     8341 taaacatcac aatcacgccg atgatggtca atgcacctga gaataagtaa cctttacggc
     8401 ggttgcccat gaggttccat ttcacatttt tgaaagcatg ttttgtgaag ctggtataat
     8461 attttacagg gcgatctttc ttcaccattg catcaataat gaggcgggaa ataagtatgg
     8521 atgaaaagag agatgcgatg ataccgatgg taagcactac tgcgaatcct ttcaccggac
     8581 ctgttccgaa attcatcaga atgatggcga tgatgagggt agtcaagtta ccgtcgatga
     8641 tggcggaata agatcctgaa taaccatcgg caactgcttt aatcatgctc ttacctttat
     8701 ctaattcttc gcgaatacgt tcataaatga ttacgttcgc atccacagcc atacccatgg
     8761 taagtacgag acctgcgata cccggcaatg tgagtgttgc acccattgat gccagagcac
     8821 cgaagatgaa gaagatattc gcaaacaagg cgatatcagc tatcataccg gcagtagagt
     8881 agtagatcac catgaaaatg attaccagta taagacctgc aatcattgac aataaaccta
     8941 cacggatgga ttctgcacct aaagaaggtc caaccgattc ttcttgtacg atacgagaag
     9001 gaacgggtaa tttacctact tcaagaatgt ttgaaaggtc ttgagcttct tcagcggtaa
     9061 agcttcctga gattgaagag cgaccgcttg gaatagcctc atttacgcgt ggagcagaat
     9121 atactttatt atcaagtgcg atggcaataa aattaccaac gttagcctcg gttacttttt
     9181 tccaaatctg cgcaccttca gtgttcatgc tcagcgagat cacaatattt tgaacctggt
     9241 cgcggtcggg agaagcattt ttcactacgc taccgtcgag cagtggctga tcatcatcac
     9301 cgttgctctt gatagcatat acatcataat ttcttgaacc ttttgccggt ttagcactca
     9361 gcatgaattt ggtaccttca gggaaaacgt tggcaacctc ttccatacgg aaatagttca
     9421 tcaatttgaa ggtgtcatct tcagcaacca taccgatagc tgccggatct gaacgctcca
     9481 ataataatga tgacaaaggg ccgaggtctt cagtctgagc ttcttcatta tctgctaaaa
     9541 gtgtgtcatc attagcagct aaactgtcat caccggcagg agttgttgct gcaaccgccg
     9601 gagaatctac aggtgaagta ttttctgtaa ctccagtatc gccggaaaca ggggcttcaa
     9661 tatcttctag ctgagttaaa acattatttg cctggttgat agcgccacca acttcttcat
     9721 aacgccaaac gccccagaat tgaaggtttg ctgttgattg gaggagttcg cgggcacgtt
     9781 ttggattgtc gacaccgggc aattcaacca tgatgcgacc ggtgcttttt tgcagggtaa
     9841 cataaggctg ggtaacgccg aatttgtcga tacgttcctt gagtttggta taagttgatt
     9901 caacaccatc atcagcagat tttttcaacc agctgattac agcgtcgtta tcagagttgc
     9961 gcggcagatc gtcaacattt gagctgtttg caaataaggt cactaattgt gcacccggat
    10021 ttgctgtgag gtaattatcg gcaaacaatt caataagatc gccttgtcct tgagcttctt
    10081 gtaaacgagc ctgatcgagt gctttaatga atttttggtt tttgggatca gaagtaagct
    10141 gtttcatcag atcgtacttc gatacttcca ttaccacgct cataccgcct tgaagatcga
    10201 gacccaggtt gatggcacgt ttttgcacat catcataatt gtacttcgca attccgatgt
    10261 tgtaaacgat tttgtcggca ttttgcgcca ggtaatcgct ttttgctgta gaaattgctt
    10321 ctgttctcgc ctgacctttt aaagtcgggt caactgcggc ttgggctgct tcgtcggctt
    10381 ttttgctttc acggctagca acgagtgtga atgataattg gtagatggtc acgaggatca
    10441 tcgctgccag aaagaatttt actaaacctt ttccctgcat aactgtaatt gttgtgtttt
    10501 gattttaggc ctgtacataa tacgggcaca cctttttcgt caaaggcggc tgcaaatata
    10561 gggtgaacgt gtgaagtttt ctactctgat gctatgtttt tcagggcttt gcgatatgga
    10621 aactccctaa aacagaaatg ccgctcataa agcggcattt ctgaatgatt tgaagggctt
    10681 tgtattattc ggctttaacc gctttggtcg taccaataac ttcgttgttt tttagcagtt
    10741 ctatcgtgta aatgcccgca gggagctgat ctgcagcgat aaccatcact tgttcgccat
    10801 ttgtttgaag cgtgcgaata atgcgaccgg tgatatcgat gatgcggata tctgttgcat
    10861 ttgcagcctc gatttcaaca gagaataagg tgttaaacgg atttgggcta actgtaaatc
    10921 cggcaggtga tacttcatct atagcattac agagattaac ggtaacatat ccggttgtag
    10981 tgctttctga ctcgccaaca atgtttgagg caaccagcgt aacatcataa gttccgggag
    11041 ttgcgtatac tacggtaggg ttttgaagcg tagatgtagc aggtgttccg ccggggaagg
    11101 tccagctcca ggaggtggca ccaaccgaca tgtcatcgaa cacaacagtt aacggtccgc
    11161 agccggtggt cacatcagag ccaaaggcag tagttggttc gctggtgata ttgtcgtaaa
    11221 ccataaacag gtctacacct gcttctacaa ggtgtccgga agcagattga tctgccgtgc
    11281 ggaagcgaac atgcatatcg gaggtgatat caatataggt tgacaagttt acttcaacat
    11341 tcacccattc gctgagttcg gtagaagaac tgttgatagc atctatcaat acttcttcaa
    11401 tgccgttgct tacaaagatg cgaagtgtgt cgtttggagt tcctgttccg ccgccattaa
    11461 agaaccaacg gtcgtaagag agcacaggat cagcataacc ggtgaggtcc attgaaggtg
    11521 aaataaggtt tacaatgcca ttgtctacat cgtcggttcc taccgttccg cccggttcat
    11581 ttccggtaac ataggcctga tcaccataat cgtcagtaac gtcgaagtct ggatttgagg
    11641 catcaggacc ggcaaaagtt ccattcggtt cgccgcgttc ccacatacct acatcggtag
    11701 ttccggaggt agtccagtcg ttagcaaaaa tgaaatcatc ataatatcct ttgctgaggg
    11761 tgatggtgta agttccgcct gcagaaacag ttacgttaat gccatttgtt acatagcccc
    11821 aatcacctgc tatgaagtcg taatcatctt catacatatc aaatgagaag ttaccggagg
    11881 cgtcggtagt tcctgagaag ttatagagtg cattctggaa aagcacgtca gctcctgcaa
    11941 ttcctgcgcc tgttgcggca tccagtactt gacctgttat agtataagat ggaagtggtg
    12001 tcagttctgc atcaacaata gcaacaatgc cattttccat aactacaccg gttacagttt
    12061 caggcaggta gccggtttta ttgaacgtga aatcatatgt tccggcatca agcacaccgc
    12121 catgataatc acctatgagg tcagaagtag catctaccac tcctgtaaag ttcacagtag
    12181 cgccaaatat cggagcaccg gtagacgcat cagtaacatt gccttccaca taacaggctt
    12241 tcacataagt tggtccaacg atgaacaagc cttcttccat atcggaaacg ataatatttc
    12301 cggaagggag gtaaggccat gaaccccaag cgccgttgaa tccgtcgccg gcgaatggag
    12361 aggtgtcgta ttcgccgacc ttaatcatgt tatcaggata tgtcgcgtct gtaatcacaa
    12421 cgccatcgcg gtaataagcg gttacaagga aatcgccgag gaccattacg ttgtgcggaa
    12481 tagagttggt tccgggatta gcctgaaatt gatcaagttc tttgatatcg gtaaggtcgg
    12541 aaacgtcgta agaagctacg gtagcattgg taacctcatc ggtagtaaag aggtaattgc
    12601 catcatcggt gagccaggtg ttgtgtgtga agttatccgg ggttgactgt tgggctttga
    12661 gcacaggaga tgttttatca gaaatatccc ataccgagaa ccagccatta tagatatttc
    12721 ctgcccagag ggtatcgcct ctcacatagc catcgtggat gtaccacata tccacttcgc
    12781 ctgcaaattc agggtccata ggatttgcgt tggcatcgag aatttgatcg ccgccggctc
    12841 cgatattcga accaaagatg tgcacaaatc cattttcatc gcagaaaacg gtatggcctg
    12901 tgctgagtcc gattccgcag ttggtatagt tataagaaac cgaagcaggc aatccgctca
    12961 ggtctatgca gagcaatccg ccgccggctt cagtcactac ataagcgtag tgatcccatg
    13021 tgcgtacttc gcgccagatg ctgttattgc cgttgacaaa cgcaacttct gtagggtctg
    13081 tagggtcggt aatgtcaacg atagaagtgc cgtcatatgt tcctacgata gcatattcat
    13141 ttcctgcgcc atcagaccag ccggtgaggt ttgaaagctg atcggcataa gggagatgac
    13201 ctaataattc cgtgttcaat tgggcattgg ccacgccaca tatgcaaagc atacccatta
    13261 aaagaggagt aacttgttta atcattctaa ttaaaagttt aatgtagaat tcaaagatag
    13321 agaaaaaccg cagcctgcaa gtgttggaga tgttaatgca gaaaaatatg tcattacaga
    13381 aacaattccc gcttgtgctt taccggaata atctaaaggc ggggggagcg tggagtacga
    13441 tagcgaatac aaaactatta gattttgaat gccggtgtta attataaaaa tataattccg
    13501 ccaagcattt tgacggactt atgttatatt ttgaactatt aattacttgg ttttacaagt
    13561 gctgataccg aaaggtgcat acagggggca aaagcgcacc aatgcggtta agaggaacac
    13621 gccaccaaga attactaaaa caagtccaag tgtgccggta acggtgtttg taaaataaag
    13681 taaagcgaaa acgattgcga gaataacgcg aataatgcgg tcggttgagc ccatgttctt
    13741 tttcataata cgtagtttat tgtttgatga gttgaatgac caatgcgatc agggttgcta
    13801 ttccgataca gctgaggcac atgagcaccc atttcatata tcgtttctgt tgcattgata
    13861 agtcaaaggt agaccgaagc cggggccatg gcttgtgact gtggtcacat caaacaagga
    13921 tttttattcc tgcgggtgtt tgttgcagtt tcccttccaa ttcaagtttt ttcaaaaggc
    13981 gggaaattac ttctctgctg cttgctaggt cggctgcaat ttcgcgatgt gtgatattga
    14041 ctatgttatt tcccgtgatc ttcacttttt gttcgaggta gtgcatgatg cggttatcca
    14101 ttttctcgta gataagtccg atggtatcta ttaaatcgcg atatcgatga ttatacagat
    14161 tgagaaataa catattgagt gaagggtagg cgataagcca ttgtcggaca tcatcaacgg
    14221 caagcaagag catggtagtt tctgtttctg tgaatgcggc aatattactt ggttcatttg
    14281 acagactgaa ggcaaatgac atgatacaac tatcgccggg cttgatgtaa tacaacaaca
    14341 tatccttatc gccgctaccg gtaattactt ttagcaagcc attcatcaca acaggtattg
    14401 ttttgatata ttgaccttcc cgcaaaattt gttgttgcgg aggaatggtg acctcttgag
    14461 cgcgctcaat gatttcccgg ataagtgctt cttccagtaa gggtagtttt tgttgcaggc
    14521 ttaatacatc catcatgatt ttcgggattt ttttcgaagg gattttaatt tctctttttt
    14581 tgcaggtgac aatttttcag cagcggcgct aaaggcaatg ctactgatgg ctgcatggtt
    14641 atcgaccaga aatgcataag tttcagtcgg ataaataatt cctgtttctc tcagacacca
    14701 tccgacgcct ttttgcacga acacatcttt gtcttttaag agaggtttca ccaatgcgat
    14761 gattttattg aaagtcggat atttttttct gaaacgtgca taatcaagga ggctgaggac
    14821 agattgtcgt cgctcccagc tgaaatcgga cttattccat ttttgcagaa cgggatatac
    14881 cattgcagga tgactttcca tcagtgctgc aaaataatgc gtaaggctat cgctatgcgc
    14941 ccaattgtcg acacgctgca accattcgca catttcgttg aagaagaatt ccgggttggc
    15001 gaggcgaata tatttatcac aaaaaaatat tgcctgtgtc atggtatcga aattatctgt
    15061 tgtatgccag atatatttcc agatgatgta ttgttcgtct gcttccaggt gactaaaact
    15121 aaatccattg cgggataatt ttctggcaac aggcattttg acaccactga cagtaaatgc
    15181 agtgccaata taatgttgca ggtcgaatcc tttgaaagaa gatttggatg gttctatctt
    15241 atggatgcaa ttttcaactt cttttctata ggctatcagt tgtttttcgg tcatagcatt
    15301 ctaatttatt tctgtagcag cataatatta acaaatctta acctccctca atgataatag
    15361 aaataaattt atcctgaaaa cgatcaacta tgcaaaagcg aattactcaa ttactctgtg
    15421 gggtgttatt gacaaccaca cctttattgt caaacgcaca gctcacactt ccgcccagtg
    15481 gtgataatca gcaatcggcg gtaagtcaat ggatggggcc tgtgcgtgtc gacatcacct
    15541 attcctcgcc cgatgtgcat gcgccggatg gcagtgaccg gaccggacat atttggggag
    15601 aactggtgcc ctatggcatg gtgaatttgc agtttggata ttccaatgaa aatcatcctt
    15661 ctccatggcg tggaggtgcg aatatgaata ctaccatttc tttttcgcat gatgtcttgg
    15721 taaacggcag tgccttaaaa gccggaactt atggattgca ttttattccg aataaagatg
    15781 aatggacggt gatattttcg tcgaattcaa catcgtgggg aagttttttc tataagcctg
    15841 aagaagatgt gttgcgtgtg cagacgaagc cggccgcaaa taattatcac gagtggctta
    15901 cctatgaatt taccgacagg cagactgatg catgcaccgt tgaattgatg tgggaattat
    15961 tgcgtgtgcc gatttccatt aaggtgcctg atatcaaaca agtgtatgta gaaaatttca
    16021 ggaagcaatt gcggtctgat gtaggattta attatcaagc gtgggtagat gcggcaaatt
    16081 attgtgttgc taataatgtg aatttacagg aggcattgac atgggcgaat tatgcaatag
    16141 aagagccatt tttcggaaga gtgaatttcg caaccttaag ttgtaaagct agtgtgttga
    16201 aggccttggg caaaactgtt cagagtgaca gtattatgaa ggtggccatt gcagatccta
    16261 cggcgtcttt aatggatgtg catttttatg caagaggatt gcaagcagag aaaaagaata
    16321 aggaagcgct ggagatattt ttaattaatt atcaaaagca tcctgaatcg ccggtgacaa
    16381 atttgggatt ggcacgggga tattctgcta atggagatta taagaatgca ttaaaatatg
    16441 caaaagcggc acagaaatta aatcctgatc cacaggtaaa gcaaaccttg gaaaatgcga
    16501 ttaaaacgct tgaagccgga aaggatttta attaagcatt acaacaataa aaaaaaatcc
    16561 cgcctgttgt tacagacggg attttttttt gaacaaaaat tcgattattt accggcaatt
    16621 ttcatttttt caaaggtgtc atccttcaac gcttcgagtg cgcgaaggta tgcttcatcg
    16681 tcggagttgc tcactatcca ataagcctga aagtcataca ggtcgcgtgc aatcaccgac
    16741 ttgataacgt ttgccagttt tgcttcacag agggagaaag tgctatcgtc tttttttgcg
    16801 ctatcctttt cagcataggt aaagaattcg ttccacagtg cttcatctac attaaactta
    16861 ttgctaaatg cgtagacatc gctataagat ttattcaatt tttcgcgatt ggcatttacg
    16921 tatgtaagtg cgaattgatt cacgattcct tttcctacta cttcggcata ataatcggta
    16981 gcccagctgg tatcgagttt aataaagata tcgggcatga taccacctcc accgtatacc
    17041 acacgtttgc cgctggtata aaatgtaagt gaatcaggat agtggaaagt tgtatcggca
    17101 gtaagttcgc catcgcgata gcgctcattt aattcattgt gataagcatc gataccgtct
    17161 tcataggatt tctgaataca gcgacctgaa ggcgtgtagt atcgggaaac ggtgagtcgc
    17221 atagcagagc cgtcggtaag gctgaaactg cgttgcacaa gcccttttcc gaagctgcgg
    17281 cgaccgatca ccagcgcgcg atcccaatct tgcaaagcgc cggtaacgat ttcacttgct
    17341 gaagcgctgc cttcgtcgat gagcaccaca actttgccat cctgcagcat tccatttcca
    17401 cgagagagag cgtcctgacg agggtaagca cgaccggcgg tatacacgat taatttattt
    17461 gcagggagga attcgttcgc catatcgata gctgcgttga gataaccacc gccattgcca
    17521 cgcagatcga ggatgagatt tttcatgcct gaagatttta attctttcac tgcggcttga
    17581 aattcttcgg tagtggttgc agcgaatttg cttacacgga tatatccggt ttcgggactc
    17641 gccatatatt tcgcatcgat gctgtataat ggaattacat cgcgcgtgat tttaaattcg
    17701 agcggttttt tggcgccttt gcgttcgatg tatacacgca catcagtgcc ttttttccca
    17761 cgcaagcgat cggtaacgcc tttattcgac attccgattc cggcaacatt ttcttcattt
    17821 atctgtacga ttttatcacc tgcaagtatg ccgactttct cacttggtcc gccggaaatt
    17881 acactcacca cgaaaatggt atcgcgcagc aaattgaatt gaattccaac gccttcaaaa
    17941 ttaccttcaa gcggttcatt tgcttctttt aattcatctt ccgtaaaata aacagagtgt
    18001 ggatcaagtt gtgcaagcac ttcgattatt gcatcttcaa ccagtttgcc ggcatcgaca
    18061 tcgtccatat atctatcgtc tacgatagaa aggaatcggt cgaatttttc aagcgcctca
    18121 gcttcagtga gctttggagc ctcggtttcg gttgttgttg cagttgattg cccaaatgcg
    18181 ttgccgacaa agaaaattgc cagcagaagg gataacattc tcatcttcac catagattgt
    18241 tcaagtattt gattgcctta aaattaggtt gattttaaat tataaacgat atccgtgcca
    18301 aaatgggttc cttttaacaa gttttaaccc cggtcttaat aaaatgcagg gttttgtgca
    18361 tatggtgata actattggaa cattccacaa acaatggtgg ttactttgca tagtatctca
    18421 acagaacttt gggctcctcg acacatagca gggcggtatt tttcatggtg ctttccacct
    18481 ttttatttgc ggtgatgaat gtgatcgtga agtatttgca tcgcttacca tcgcacgaat
    18541 tggtgctttt ccgttccctg gtcatgttcg ttattactgc cggaatgctt tacagggcta
    18601 agttgcagcc attcaggtat cagccgagat ggatattgat aggtcgcggt gtagcaggtt
    18661 cgcttgcatt gttgatctat tttttcacga tacagcatct gccattggct tcggcggtaa
    18721 cgcttgggta tttatcgccc attttcacca tcatttttgc ttctttttta ctcaaagagg
    18781 gtatgcgttg ggtgcagtgg gtgttctttt tggtatcgtt tgcaggtgtg gtaatcatgc
    18841 atggatttga atcggcagaa gatacaccct tggtgctttt aggtgtgttg ggtgctgcct
    18901 ttagcgggtt ggcttataat gcattaagaa aatcggccgg tacggtgcct acattggtag
    18961 tagtgttctt tttgcccttg tgtaccattc cattggtgat acctttttgt ttgcataatt
    19021 ggattatgcc ggtaggatat gaatggttgt tgattattgc aatgggagcg gtaacgcagg
    19081 cagctcagat ttatatgacc aaagcatatc agctggccaa ggccggagcg atagccaatt
    19141 atgcctacct cggggtggtg tatgccctga tattaggata ttttatattt gatgagcgtt
    19201 ttgcctttat tggcgtgata ggaatcggga ttgtgatatt ggggataatc ctcaattatt
    19261 tttacgtcaa tcgcgttact tccttgaagc gattcgtggt ttatttccgc tggtttccgg
    19321 gcctttgatg taagtattgg ttttatcaat actcgaattc gccgtaagct gatttaaccg
    19381 taagcttttt gttttcagat ggcatcacat gaccgatgat ttgagcttca atatcaaaac
    19441 tttttgcgat attgattagt gattctgccg tattctgatc ggtatagatt tctagtcggc
    19501 aacccatatt aaaagttcgg tacatttctt tccatgaagt gccggattcc tgctgaataa
    19561 tgtcaaaaag tggaggagtt gcgaagaggt tatcttttac aatgtgcact ttatcggcaa
    19621 agtgcagcac tttagtttgt ccgccaccgc tgcaatggat cataccctta attgcatcac
    19681 tatgattttc gagcatggtg cgaattacag ggaggtaagt tcgagtaggg gatagcacca
    19741 actttccgag tgtaattttc tcattattga cagtgatttc atcggtgagg gatttacttc
    19801 cggaatacac tacttctctt ggaatttcag gactgaaact ttcgggatat ttatcggcat
    19861 attgatgatg gaaaacatcg tgtcgggcgc tggtaagacc gttgctgccc atacctccgt
    19921 tgtaagcagt ttcataaatt gatttcccgt agcttgccag gccaacaatc acttgtccgg
    19981 gtttaatatc gttgacgatc aatttactgc gatctatgcg accgaaagcc gtgataccga
    20041 catcgatagt gcgcacgata tcgccaacat cggcagtttc gccaccggca tggtgaattt
    20101 gcacaccatg caattgcata tttgcgagga aattatttgt tccgttgatg acagcagcga
    20161 ttacatcggc cgggatgatg cttttatttc ttccgatagt tgagctgata ataatattat
    20221 cggtgatgcc aacgcaggcc atatcatcca gattcatgac tagtgcatcc tgggctatac
    20281 cttcccatac cgataggtcg ccggtttctt tccagtagat ataagccaaa gatgttttgg
    20341 tgcctgcagt atcggcatgc atgacattac aaaaggcggg atcaccggca atcagatcgg
    20401 gtaagatctt gcaaaaagcg ttgggaaaca ggcctttatc catgtttttg atggctttgt
    20461 gcacttcttc tttctgcgac gacacgccac gcaaggcata acgatcctgt tccatgctgc
    20521 aaaattacat aaatgctttg tgcctctaat gttacccaaa caataaatta acattaactt
    20581 aaacgaataa tcaggaaagg cctgtttatt gcccgtatct tagtcctgaa tatatttgtg
    20641 atgaacagca tgtaatgtta tggcggggaa aaaacagaag aaagagaagc caaagtttat
    20701 caatcgtgac ctcagttggt tgtcgttcaa tgagcgtgtt ttgcaggaag cagaggacaa
    20761 gcgggtgcct ttgaatgaca ggatcaggtt tttgggcatt ttttcgaata atcaggatga
    20821 gttgtttcga gtgcgtgttg cggtattgcg caggatgatg cgattgcagc gcggcgatct
    20881 gaagcaggtg gatatgaatc catcgaaggt gttaaatcag attcagaaga aagtgttggt
    20941 attgaagaac aggtttgaca atgcttattt gcagattttg gaggaattga agaagcacaa
    21001 gatctatatt ttaaatgaga aagagataag tacgcagcag ggtgaaatca taaaaaatta
    21061 ttttaatcag catgtaaggc agtggttatt tccgattatg atagatgaat cgaagaagat
    21121 gcctttatta aatgataaaa gtatttatct ggcgatcagg atggtgaaga aggataagcc
    21181 gttggcaaca cgatattcgc ttatagaagt gccggatggt gacctcccaa gattttttgt
    21241 gttacctgat gacaaagaga aaaagtttgt gatgttgttg gatgatatta tcaggtataa
    21301 tctggatagt atttattata tttttaatta cgacagcatc gaggcatata cgatcaaaat
    21361 tacgcgtgat gcggagttgg atatcagtac agatttgtcg caaagtctgc tcgaaatcat
    21421 acagaaaagt gtgaagcgca ggaataaagg tgaaacaaca agattcattt atgatgcgca
    21481 gatgccggaa gagatgttgc aattttttgc acggaagatg aaattacagc gtcaggatcc
    21541 gataattccc ggtgcgcgct atcacaattt caaagacttc atgaaattcc ctgatctggg
    21601 aagagatgtg ttgacatatc ctgaattagt tccgcatcat catccattgt tgaacaccta
    21661 ttcgagtatt acttcggtat taagaaagcg tgatgtgtta ttgcattttc cgtatcagag
    21721 ttttgatcat attatagatt tattgcgcga ggcggctatc aatccgaatg tcaccacaat
    21781 caaaatgaca ttgtatcgag ttgcgcggca ttcaagtgtg gtaatgagtt tgatcaatgc
    21841 tgctcgaaat ggcaagcagg taacggtagt tatggaattg caggcgaggt ttgatgaaga
    21901 aaataatatt ttctgggcca acagattgca ggaggaaggt gtgagggtgt tgtttggaag
    21961 caataatctg aaagtgcatt ctaaattgtg tttgatcaat catcatcacg aaggaaagga
    22021 tgtgcaatat gccattgtag gaacaggtaa tttcaatgag aatacggcaa aggtgtatac
    22081 agatttattt ctgctgacag caaagcccga ggtgacgaag gaggtgaaga agatattcga
    22141 catttttgaa aataagtttg aattagggaa gtataagcat ttattggtag caccaattaa
    22201 tttaaggtcg cgattgaaat tgctcattaa cagagaaatt aaattcgcca aagagaaaaa
    22261 aaaggcagct atttttttga aagtgaataa cctggtcgat aaggagatca taaagaaatt
    22321 atatgaggct agtatggctg gcgttcagat taaattaatc gtgcgtggcg cttgttcaat
    22381 tgttccgggt gttgaggggt tgagtgataa tatcgaagcc atcagtgtat tagataggtt
    22441 tttagagcat ccgagggtgt ttgcatttca caataatggt aaagaggaag tgttcatcgg
    22501 atcggcagat attatggagc gcaacttaga tcatcgtata gaagtaatgg tacaggtatt
    22561 ggataaggat atcagaaaga tgataaaaga catattgacc atacaatggg gcgacaatga
    22621 aaaggcgcgt ttgctggggc atgggcattt gaatgattat gtcaaagtgt tgccaggaga
    22681 aaagcaagta aggagtcaga tggcgctata tgattatttt gaaaaatcgc gcctggcatc
    22741 ggcgttagaa aaaaaggaag gtaataagta aatatgaaat ttgcagcgat agatattgga
    22801 agtaatgcag tcagattatt gattgaaaat gtatatgaga catcttcagg accggtattt
    22861 cataaagaca gtttgatcag aatacccgtc cgattgggag aggacgcatt tttggatggc
    22921 gctatttctg aggagaaagt gcagatgctt gtcaaagcga tggttgccat gcgacatatc
    22981 attgacatcc acaaagtgac tgctataaaa gttgcagcca catcagcgat gcgtgaagct
    23041 gctaatagtg gagatatcat taatcgggtt atggaagctt ccgggattca aattgaagtg
    23101 ataacaggcg atagtgaagg caaagccatt atgcaattgc cattagaaca aattggatta
    23161 aatccggcat tacattattt atatattgat gttggtggag gtagtactga attagcattt
    23221 attggcaaag gagatttaac ggcgaacagg tcgtttaaca ttggcacgtt acggttgtta
    23281 aatgatcaag ttagtgatgc agactggaag gagatacctg attggataaa agcccataag
    23341 catcatggat ttgatttgca tgcaataggt gttggaggca atattaacca tgtaatgaag
    23401 cattacggta aagcaggcaa gccatatatt tcgatggcaa tattgaagcg cgtgatgatg
    23461 tatattgaaa gtttgagtat gggtgaacgg gtacgaacat tgggattaaa acctgatcgc
    23521 gctgatgtga tcgttccggc tttgaaaatc tatatcaacg tgatgaaatg ggcgggaatt
    23581 gaaaaaatat atgttcccaa gcagggattg agcgatgggc ttatcagtca gttgtatcac
    23641 gagtggaaat caaaggtggt cactccctga ttagtttaag cgtccatatt tgcatatcgg
    23701 catcagggct tgattgatcg atattttttt cagcctccat atttctcagg aatccatttt
    23761 tttgcatggc atgttgagca gtaaaatttt ggtgatgtgt ccagccttca attatagtga
    23821 atggaagttt ttccattgca aatgtgatga cacgttcaat agcttctgtg gcataaccat
    23881 gattccatgc atttttgatg agcacgaaac ctagttctgc ggtatatgat tcgtaattat
    23941 aattccagag gcaaatactg ccaaggtagg tgttttgttt atcggtaatt atccaataat
    24001 cggaaattcc ggtgctggtg ttatggatga tggttcgcaa aagagtttcc acttcggagg
    24061 gatcctttgg tggcgtcatc gggacgaatt gattcacttc ggcatcagtt cgttgcagga
    24121 acatattgaa tgcatcattt aatgatggat tgcgaagtat tagtcgatcc gtaaatattg
    24181 ttggagaaac gtaattgtca gacctcgtca ttttaatttt ggttgatttg tttgtgttgt
    24241 cctaatattt tggcttgctt cagctacagt ttgggctata cgtcgttttc tggtttcttc
    24301 ttttttggcg ctttgcagcc attgaaggat agctcgtttt gttgaagcag gaaaggcatt
    24361 aaaatgttta agagctgttt tgtttttttt cagcgccgat tgaaggtcgg gaggaattgc
    24421 cagtgtttcg acttcattga gtgtatccca ggtcccggtg ctttttgcaa gttggatcat
    24481 ttcgagtcct gcaggcgcta ttttcccttc gttgagtaat cgctctactt ttttcttatt
    24541 gatgtcactc caaccacttt ttggcttgcg ttttgcgaaa tattggtaga agctgttttc
    24601 gtctctgctg tttcgcacgg aatctatcca accgaagcag atagcttcat caacggcttc
    24661 ttcccaggta agagaaggga tgccgctatt ttttttgaac atcacaagcc agaccttttc
    24721 ttccttagca tgattttttt cgagccagtt gcgccatgcg gttggagatt tgccgatgca
    24781 gattttaatg tttttgtaga gtgttggcat tgtgaggtga aagtagggta gggagttgga
    24841 ttgtcaaaat tggggggcaa acctttcgtg ttttaaacct tccatgtttc aaacctaaca
    24901 ggtcttaaac ctgtcacatt ttagacctaa aaggtttaaa acgcgacagg tttaagacct
    24961 gtttacgtga accgcttaca tcgcggtgct tactcacctc tcgccaaatt gagtttttta
    25021 ggaaattttc aaatcagagc aatttgtcat gtcacaaatc tccttttttt agcaagttgc
    25081 tttaagcgag tataccatta ttaaacctgc cttcgcaagt tatttacttg aaccattgag
    25141 aaattttata tttcttcaga tcaattcaaa atcacgggaa tagacaacat tttttcacca
    25201 tctacaatcg caatcatata tctgccagcc gacattctac ttaggtcgcc gtgtaacaaa
    25261 ttgccatcta tgatgccttt agacacaatc tgaccattta tcgaggtgat cgcaaaatct
    25321 ttgccgttat aactgtcagg catattgatt tggaattgtc ctgaggttac aaccacagtt
    25381 ggtaagttgt cggttgttgc atttgaaatg ccggattcaa catcattgtc tgaaatatta
    25441 atcatggtaa atggaatagt gtatccatca aactgaggat cgtcggtagt gatgatataa
    25501 ttaatcagag cgtaatgatc gccttctaca atagcatcat caatgatgtc gacattgaaa
    25561 tattgcattt ccatagcagt ggcctctgtc gcgaaggtga gtgattcaat taatccgaga
    25621 tcaatttgcg tatcgtcata ggttgccgag attgttacta tgtcattagg tatggtgaat
    25681 agagaaagtg aaactgataa accggttgtg ccttcggcat agaatgttgt tcccggagtg
    25741 gtgatgttga caccttgttc tgcatcatta tctaaaatat tgatagtaat gtcaggtatc
    25801 aggtagtctg acaagaatgg gtcatcggtg atcagttctg caagtatgct gccgatatga
    25861 tcgccatcta cgatgaaatt atctttcacg gcaattgtca agataattgg tgtcattgca
    25921 gtttcatcgt tttcaaaatg cagattcaca atagcgccga agcctgcacc aagatcgata
    25981 tcttcggatg gtgacaacaa aatgtcggca tcaagatcag gaacagattc taaagccacg
    26041 ctgaatggta ttcctgcttc accttcagta tgtgtaaatc catctacgct gattatcacc
    26101 gattgtggaa aatcattgtc atctattgaa attaccagcg gatcaattgt cactcctgtc
    26161 aacacaggat cgtctgtaat gatttcgaat gtgatatttc cttcatggaa gccttctatt
    26221 atttcatcat caacaatatc aatagaaaat tcgtgaggaa gtgtagcgtc caaatcattc
    26281 aataatactg atacggcaac tccagcacct gcgccaaaat ctaattgtgc atccggaata
    26341 caattaatag ttactgcaga atttggttca acatttagca taaggctcat ggttagatct
    26401 gctgtgcctt cagtgtatgc tatcgccggt ggagcggcta tttgtatgcc ttgaagcata
    26461 tcttcatcct gaattgaaat ttctgcaggc tcaattatct catcgtccaa tacaggatct
    26521 gttgtaatta tttcgaaaga tatattcgcc agcgtgattt caggacctat aatcgtatta
    26581 tttacggctg tgatattaaa aattctggga atagtagcat catccaaagg atcgaaagaa
    26641 atactattgt caattcctgc accaagacca agatctatat tagcatccgg gtgacctatg
    26701 atggtaacta atgcattcgg atttgtccat aaagacatac tcatcaaaat gtcggttgtt
    26761 ccttccatgt aagtagctgc aggtggaagt gtaactgtaa ttccctgagg aaggtcatta
    26821 tcgcttatag agaaaatata ggaagctggt gttatatcgg cataataaat atcggctgat
    26881 acaatggatt caattatcat cgcatcatga gtgccctcgg caaagaagtt gtctactgca
    26941 gtaacagtaa gtgtttgtgc tgaaggaata acatcgcctc ccgaaaaagt aattgaaaca
    27001 ggaacacctg tacccaagcc taaatcaaga taaatatcag gtgtataggt gattgttaca
    27061 tcttgggtag gtgcggatgt taacgaaact gtcattgtgg ttgattctat accttctgac
    27121 aattctgttg ttccgaaaac tacctgcaca tctactacat cattttctat aagtgtgacc
    27181 ggcaataatt cgctcgtaaa agcatcaatt gcagcatcat ctgataaaag ttcaattgtc
    27241 aatgatgaag cgatatctcc aaaataggtt gcgtcttcag gatataacaa agtcatctta
    27301 aatggtgcaa taccaacaat tcctgaaggg atagtatatg tctccgtcat atcatttcca
    27361 aatgctgaaa tataattcgg gtcggcgatt gcttgaataa gcacgtcgtt ttgtgggatt
    27421 acatttaaat gtgcaaatac agtatctgac caaatacctt catttatttc caaagcatcg
    27481 ttggtcgtga aaatagctaa gtcattgtct gtaattgtga agtcatagtg ctcatcaagt
    27541 aaggaggcaa aagctcccca tgaaacctca tctgctgtaa tggtaactcc gccaatagta
    27601 atatcttctg caatagcatc ctcatttgta gataagtgaa ttacctttgg ctcaagcgcg
    27661 gttgagtcgg catcaacaat gaaggtgtat cctatacttt ctccggcacc ggcattcaat
    27721 atcccgtatg ggagcacatt tatttccagt ggttcttgag ggatagtatt aaatgttatt
    27781 atgatagaat cttcttcggc accttcagaa atatatgttt gagttggtgg tgtaacttca
    27841 aatgatggag gtaacatcaa aatgttatat tgaatataca atccatttga ggagatacct
    27901 acgaagtcag gaagcgttcc gggatctaca tttgcgaacc tgccatagtc tgttgagaaa
    27961 aggtcatagt cttcctgaaa tgttccgggg cctgtagcta atttaaatcc tgatccacct
    28021 gaagttttat tgaatacaaa atcttcaagt ccgtccatgt tcatatcaaa cattgcagta
    28081 tgtgaaccat cagaaagtgt ggccgggtca aaagtgccta tcgcagaatc tgtatatccg
    28141 gtaccattgt caagtgccat atgcattgta ccatcaccac atacataggc aaaatcttta
    28201 tccccatcgt tatcaaaatc gctggtaaat agcgttttga catcatcaca agccgataca
    28261 aagagattgg agatatcctc aaacagcaat cctccatcca gattaataat tttcttaata
    28321 ccatttgcat cattgcggat tccaactaga tcgtcatcac catcgtcgtc aatatctaat
    28381 tgaacaaaag agttgtaata accatacgca gtcagttctg ttgtgtagct aaataaatga
    28441 tcaggatttc cggtatttgt aaaatacaat aatctgttaa gatcacctgc ctttaaacct
    28501 gcaaggtcaa agtcgccatc ctcatctccg tcaattaacc atacatttga aaatgcggta
    28561 tcgcaaaggg tgtgagtttc gaagttgccg tcgcctaaat caagatgata aaaggttcgg
    28621 gctaatgcgc cattatagga aaaactggag attatatcga aatcgccgtc gctatctata
    28681 tctttcttct ttatgttttc aggtgttggg cggcttcctg tgtaattgat attatgcata
    28741 atggtaaacg aatccaccaa atggttaaat tcaaaccatg acaataatcc attgttgaaa
    28801 gcaagtaaaa ggtcgttatc aggatctccg tcaacatcat atacgataag gtcggctacc
    28861 tcaggactta cttctaaaaa tgaagaatca taagcaccga agaaacctcc tatatttgga
    28921 cttacaatgg ctgttgtgcc ataatctccc gattcgcatt gtataaaatc aaaggttcca
    28981 tcgttattca tatcgttgaa actcatacag ctattgtaat atggtaatcc tgcaatgatg
    29041 gattcgaaat tttcgacatt cagattcaaa aatgaaccat cagattctag ccaggagatt
    29101 tcataagtac tttctcctgc aaaataattg tcatttacaa tcacaaaatc tttgtctcca
    29161 tcaaggtcta agtcgatatt taaaaatttg cgaaaatcaa agtcatccat attcccattg
    29221 tatactccgg gactgaaggt atgggtgatg gtatcaaatg tgcttacgta gaaacttccg
    29281 ttattatata aaatatcagg atggttatcg ccatttacat ctccggaata cacatcatag
    29341 cttgattcgg agttttttaa attggttgat gtaaatgtgc cattgctatt gtttctccaa
    29401 tattttacgc cactatcata gcaataaact gctaaatcaa catcattatc gccatcaagg
    29461 tcttccgcag aaaaactttc tacccatgac caagaatctg gaatcgcatg ataaactgaa
    29521 aaatttccat cgcccagttc gcgataacca agcaggtaag cgccgtcttc aaatacaaaa
    29581 tcctcattgc catcgccatt catatcaaag atccctaaac aaactcgata ggaacctgcc
    29641 actgaaaatg ttgtccattc ctgatcagtt gctcccggat ttttgagcca tcccatttca
    29701 aaaggaacgg tgcctttttg gtaccaaatg tctttccatc catcatgatc aatgtctacc
    29761 acctttaaat ccgttaggta ttcattcagc ttggctcctg tattgtgtat cgtgccaaat
    29821 tcgtaggtgc cattgccttc aaaccaatga atttggtagt atccgcctac atagtcgatg
    29881 ataccatcat tattgatatc ccctgatgcc gaacgagtga tggacggatc aataaaatcc
    29941 aatatgtagt cctgatcagg aagttgggca aatgttaggt tagtaatgat tgcaagagca
    30001 gtggttagtg taaaggtttt catttgtttg aattgacaat gtaaatatgg tggctactgc
    30061 ttgtcctcac aagacgcttt gagtgaaatg acctgttttg cctgtaaaac ctagttctga
    30121 cttagagagt gactattgtg ggtttggtgc actttacccc aaattaacat tttattagcc
    30181 tacatcaacc tagcattaac aatgaggcta cgctgttgac attcaattaa gaaatcgctt
    30241 tattaccgta ataaaatact aagaaacatc taaaactacc tcaatattcc gctaatacgc
    30301 cccatctact tttgccccca tcttttcatc acttctaaaa gaacttatca atgaaaaaac
    30361 tattactcgg atgtctcgcc tttacaggtg tattgacagc caagtcgcag gtgacaccta
    30421 tcactgtcag tggcgagatc acgacaaata ccacctggac gaaaaacaat atttatctct
    30481 taagtggatt tgtgtacgtt gaagatgggg caacattaac catcgaaccg ggtactctta
    30541 tcaaaggaga caaagattca aagggtacat taattgttac ccgcaccggc atgatcaatg
    30601 ctgccggaac agcttgtgaa ccaattgtat ttacctcaaa tcaacctgcc ggttcgcgta
    30661 cttatggcga ctggggtggg gtgattattc ttggtgaagc gccaattaac gttcccggtg
    30721 gaacagctgt tattgaaggt ggtgttgata cgcctgaagg cgatggcgtt tatggtggtg
    30781 cgcttcctgc tgataactca ggtaccttca cctatgtgcg tatcgaattc cccggaattc
    30841 catttttgcc gggaaatgaa ataaacggac ttaccatggg tggtgttggt tcaggtacaa
    30901 ctattaacca catccaggtt tcttattccg gcgatgattc atttgaatgg ttcggtggac
    30961 gtgtaaactc aaaatatcta attgcatatc gcggatggga tgatgatttt gataccgatt
    31021 acggttatcg tggtaaagga cagtttttct acgctttacg cgatccgaat attgccgatg
    31081 tttccggatc aaacggccag gaacaagata atgatgctac cggttctgct aacacaccta
    31141 ccagccgtcc attattctca aacatcacta ttgcaggtcc aaaagtaaat acaggtgatg
    31201 tagtgaatgt aaactacaaa cgcggtgctc acgatcgtcg caatactatg gcttcaacct
    31261 ataattctat tatcatgggt taccctgaat gtggattgaa aattgaaggt gtgaatactt
    31321 cactcaatgc ccaaaatgat tctttgcaat ggaaaaagaa catcctttca ggtcatggtg
    31381 atgattatat ctgtactact tgtgatgttg gttttaatat cgatacttgg gcatcaacaa
    31441 atggaaatgc aacatatgca acaaatgcac ttgtaggtct tgttgatcct ttcaacttaa
    31501 gcaatccaga cccacgtcca actgcgggtt ctgcaccaac cactaatgga acttattttt
    31561 caggtttgtt ggcagatcca ttttttactg cgacatcata tgcaggagcc ttctctacaa
    31621 caaataactg gacagatgag tcatgggtga atttcgattg tcaaaatact ccttataata
    31681 cgcctggtat tagcaacaat cctgttgtat cagcagtagt tacaagcatg caatgtgcaa
    31741 acacaggtgc tattgatgtg agtgttagcg gcggaatagg ttcatatacc tatttgtgga
    31801 acgatggtgc aacaacccaa gatcgtaccg gattgaatgc aggttcatat actgtaactg
    31861 taaccagcgg cacatgtact acaactgcta cctacacagt atctgatatt acaatcacta
    31921 aaccaacaag ccttaccact acacaggcta ctcattgctc aattaaatta aattgggctg
    31981 cagtgcccgg agcaggttca tatgaagtgc gttaccgtat taccggtggt acttattcat
    32041 cacctgtaaa cgttggcgct gttacatctt atacatttac aggattagca gcttctactt
    32101 cttatgattt gcaagtacgt gcgaaatgtc ctaccggagc tgaaaaatca gcttatgcaa
    32161 agaaaacaag ttcgacagta gcatgtggtg cacctacatc gcagtctgca ggttctatta
    32221 ctgcaaactc tgctgttatt agttggtcaa ttccttgctc cgtaacaaat tacaatctcc
    32281 aataccgtaa aaatccaact aaagcttgga caacagtatc gggtattgca actacttctt
    32341 atacattaac cggtttgtta cctagctcta cctatgaata ccgcgttgct tcaaattgtg
    32401 gtggtacatt atcagcctat accggtttct ccaacttcgt aacattgcca ttccgtttgg
    32461 aaggtgaagt tgaaatgcct gccattgatg cagatatgga aatctatccg aatcctgcag
    32521 ttgatgaagt taacatggta atcaatgcgc aaggcgttgt gaatgtgatt atcaccaata
    32581 tgttaggcca ggttgtttac aatcttgaaa atgttgaagt aaatggcaca gaaactgtaa
    32641 gcctttctct tgaaggattt gatacaggta tctaccacgt tactattcag aatgatgtaa
    32701 tcaaactcac cgatcaatta gtagtaagta aataaaatgt taattgtagt ttaaagtaaa
    32761 tggcctcctg ttaacgcagg ggccattttt cgtttacatg ggtttgacac aagtctaatg
    32821 ttaacctaat aattacgtaa gatgacaccc tgaactttgc atcaaaatcc taattgtatg
    32881 caacgtttaa tttttcttgc ttttctcctg ataactgcaa ctattgcggg gaaagcacaa
    32941 aacggaatga tttcaggggt cattatcgat ggcaaaactg ctgaaacgct cattggcgca
    33001 aacgtattga taaaagaaaa agccggaaca ggcagtagca ctgatattga tggaagatat
    33061 aaaattgatg accttgaacc gggcacctat accgttgaaa taagctacat ctcttatgat
    33121 accaaaacag ttccgggtgt agaagtaaaa ccgggacaag ttacaagctt agatgtaaca
    33181 ttaacatcag gtttgatgga gctgacagtt gttgaagtga aagctcgccc gcgacaagaa
    33241 tcgctcaata ctgttttggc atttcagcaa aaaaatatcg ctccttcgga agtaatatcc
    33301 cgcgatatca ttacaagtac tcctgataaa aataccggtg aagtattaaa acgcatgagt
    33361 ggaaccacta ttactgatgg caagtttgtg gtaataagag gtcttagcga tagatataat
    33421 cttgcacttg tgaatggaaa tcttcttcct tccactgagc ccgatcgaaa aacatttgca
    33481 tttgatttgt ttccatctgc tatgttggat aatctaattg ttattaaagc agcacaaccg
    33541 aatttgccgg gcgattttgc aggtggaatc atcatactaa acacgcgcga tattccggat
    33601 aaaccattta tttctatttc agccggtgca ggctataaca ccatttcaac cttcaaagaa
    33661 tattatacct ataccggtgg taaaactgac tggctgggca tagatgatgg cacgcgtcag
    33721 ttacctgcag ggtttccggt aagtcaggat ttatttgacg atttgaca
//
LOCUS       DAOQOU010000013        32823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_598_length_32823_cov_6.634979, whole genome shotgun sequence.
ACCESSION   DAOQOU010000013 DAOQOU010000000
VERSION     DAOQOU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 32823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32823
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_32823_cov_6.634979"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLM90_02120"
     CDS             <1..274
                     /locus_tag="PLM90_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88191.1"
                     /translation="CQVLSIRSPTMHGVTLYPNPVDDILMIAGTIDNIVSVSVVNTIG
                     VELFCQQSCTDLNYVLTTASLAAGNYYCICKSDIGTRIILPFIVEH"
     gene            302..847
                     /locus_tag="PLM90_02125"
     CDS             302..847
                     /locus_tag="PLM90_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HMZ88192.1"
                     /translation="MRLTYRLSEQDFLNCHLFFTSRSEATRKARKRNRLLMPGIYIVL
                     GLATLFIGDPILSGLLIVAGVLWYFFFPGYEGKRIATRMQKHLQENLRSQFDQETNLD
                     ITKEMIIDKVNTLESRIPASEIKEIYKVPGYYYFLTKTGTAIIIPRNILEDESTLNGV
                     IQEMCDDLKLPYREFAEWKWK"
     gene            complement(854..2626)
                     /locus_tag="PLM90_02130"
     CDS             complement(854..2626)
                     /locus_tag="PLM90_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88193.1"
                     /translation="MDPTFDGDGIVTTAFADGYDVIFAMELQPDGKILGAGGTNFIAG
                     TETDFVLLRYLTDGSLDESFGDGGIVTTDFELAHDLANAMYLMPDGRILAAGYSGNDW
                     AIARYLPDGELDTTFSNDGKLLVDFDLENDQAFGIATQSDGKIVVGGFATVDGFYPRF
                     ALARFYENGDPDLSFGVDGKTTETVFGRWDQARALILRPDDRIMLVGYTQDLMNSFDM
                     TVMQFNSNGALDVSFGDDGIVRTDFDSTVNFAYCGMLQPDGKLVIAGTTDVVPDQNIA
                     MARYLTNGELDTTFGDGGRVVINVDPYQEEQIFGIAYEPDGRIIIGGEIGTYADGYDY
                     LVAKLLPDGSPDSTFSDDGIVITHLGDDWDLAYDVLIQDDTKILAGGFTNDAGDNDFA
                     VLRYFNSPYITCDTITEQPMDVIILPLATAVFNVTSPFPDAMYQWQEWDGVAWTSLED
                     VYPYSGTHTSELSIAPVDYWYDGLQFRCIVAYDICSDTSNAALLVVLLDDINELQQNL
                     SFSIFPDPVQSILNVGYTNDYATSIKWQICNALGAVCLKGDEYLEKGTDVFQINTLEN
                     LPAGIYTCSISVDGVRNTKSFIKN"
     gene            2800..4308
                     /locus_tag="PLM90_02135"
     CDS             2800..4308
                     /locus_tag="PLM90_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HMZ88194.1"
                     /translation="MQFTYRNGILKKYSILTLVLSMTCISMNGQGNGEAHRPLIHFTP
                     ETGWMNDPNGLIYYDGYYHMFYQYYPDSTVWGPMNWGHAQSLDLMTWERQPIALYPDS
                     LGWIFSGSAVIDVHNTAGFGNNAMVAIFTYHNDAIWQSGNKNTESQGLAYSTDKGATW
                     KKYVGNPVLNNTGEQDFRDPKVFWNSSIRKWNMVLAVGDHIRIFSSPDLKKWTFESDF
                     KPEEDEEVGVWECPDLFQMAVAGSSDKKWVMLMSHGYNEKDNKQSGTRYFVGDFDGHT
                     FKNTQQAQWIDVGSDFYAAATYANAPGNKRILQAWMSNWMYAQEVPATRWRSTMTLPR
                     ELALIKDKYVYAITQQWATQIINNQSEIYSKPESSDVELSGNVIPHCRITLDLEENNT
                     TSIQLSNNENEVFTILIDGTTITTTRDVYRDEDAIYPFITETAASLPGERITKLDMIF
                     DVGSCEILVNDGKLSMTTIYFPTSNFDKLSINPMDGGILRNVTIYGITPTMW"
     gene            4312..4707
                     /locus_tag="PLM90_02140"
     CDS             4312..4707
                     /locus_tag="PLM90_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HMZ88195.1"
                     /translation="MMHSPEIPTDRQTWESPIAYYWWEDEILISLSKSTLRTVETIKA
                     NVAIVQTITGNKPTPLLIYLSKSPIPDKETQSYSKTQLPLIYSSMAMVSKPGLAQFIM
                     RLLFSFQKPPIPMKSFADAEKAKRWLRSH"
     gene            complement(4708..8730)
                     /locus_tag="PLM90_02145"
     CDS             complement(4708..8730)
                     /locus_tag="PLM90_02145"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ88196.1"
                     /translation="MKRLIVLPLCGLFSLIQAQVLINEAAHTNTGIVVDEDGELPDWI
                     ELYNGGVDPVNMAMWSVTDDLAIPGKWQLPDTVLAPAAHLLIMASEKNRNAYDAEMAS
                     VDHWETAVYDDDIWEYFPADAEPDPSWRTSTAPFGWDTGKGGFGFGDDDDSTIITTDV
                     RSVYYRKTFEVADKTKLIHAIINMDYDDGYVVFINGILISEAGLTGIPAYDDLSVTAH
                     EAQMFDGGNPAYITLDSATLAGIIVDGTNVIAVQVHNKTSGSNDLTGRTFLHFAITDA
                     SVFYGIPPLWFNPTNSTELHTNFKIGLGETIALFDNAGILRDSITMQDLEAGIVKARI
                     PDAGGWCMTNIPTPDTVNSGDCYLLYAATPSITPLPGFYTGDQTVTISGVDVQYTTDG
                     SDPLLGGGISYIDPFVVTTSTVIKARSFETGKLPSALATTSLLINEPTLLPVVSISAT
                     PCDLFDLAATCPAAYDNATSFDNENPQINCVIEYFNADKTFQFAAPMKFEVAGNYSIE
                     LDQKGIQFTCDEDFNSPDNINFPLYSLDKPVIDKYHAFRLRNCDNDAELARSRDLLIN
                     RMALPTHTVAAANQNVAAFINGEYWGHYVSRERLDPYFCRDNFGANPDRVDMIKAHFS
                     GGGNYVTEAGSDTGFFNLHTYCIDHDLTDPVYYAAVLDQIDEDNWIDYFATEVYSANS
                     DWIGFIVNNIRLFRAVSPDIEWKFMLWDLGSSQGTPGAGASSNTLATAMDDNNFYIDM
                     FNALMENTEFHDHFINRFADLLNYYYTPEIANALIDANTAEIITEMNAQDDKWNTGDS
                     SFVQSKITNLRNFHNDRRGYVRDDIENYYDLNDQVDVTIDVAPAGAGYIKISTIIPET
                     LPWTGVYFDGVPVTITAIANPGYTFVNWTDNPFIATADVPAFNVNITEATTFTANFNG
                     TGISNPIIISEINYNSDSTLNAGDWIELHNTSGMEIDLTNYQFSNTIFYNDYIFPSIK
                     IAPDGYLVLAEDMIEFNAVYPDVTQVIGGFDFNLANDGDSITLIDFLGNVITSFKFND
                     KRPWPVTADNYGRTLERFEPAIDPGLPEAWFPGCVGGSPAAAFTSCLENPLVDEINYN
                     SDADSNAGDWFELMNTSADIIDIGNWTVKDKNGNAYVLPTGTQILPDSFIVIYQDAAL
                     FASRFPDVTNISGPTGFGLSSDDDIISIYDAGGVIYQSVSYDDTIPYPISPDGGGVTL
                     QIVDVLENINLASNWMESCPEGSPGMDYYFPCLVPEDTTTDTSGEDTTIVESIANYLE
                     GDILIFPNPANAQINISLPDNLQTDGLMHVYDLAGTLKETFDVSASTMQLDISNYLPG
                     VYLIQWINDTSQLQQAFIKE"
     gene            8874..9179
                     /locus_tag="PLM90_02150"
     CDS             8874..9179
                     /locus_tag="PLM90_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HMZ88197.1"
                     /translation="MSKPFATNPELVLREELAIQRTTLANQSTFLSFIRTGLYFLLGG
                     LSLTNLLKMNDAALMEVIMFSIAALLVIIGSFNYMHHQRKIARSKLQIGQVAQATRS"
     gene            9208..9504
                     /locus_tag="PLM90_02155"
     CDS             9208..9504
                     /locus_tag="PLM90_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88198.1"
                     /translation="MPKRKLYTCSKGHQFYKSSDCRSCPTCASLAKPKDGLLSLLSAP
                     ARRALLQHGVESEHMLVNFSREEILSWHGIGLSAMAILDLQIKKLKLSYKISKL"
     gene            9538..10392
                     /locus_tag="PLM90_02160"
     CDS             9538..10392
                     /locus_tag="PLM90_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ88199.1"
                     /translation="MNKLQFLSILLLATFSGFAQSPSARPFTIGVTEILHSNILNEDR
                     IINIYLPEGYSKDDSINYPVIYLLDGGADEDFIHIAGLVQFNNFPWIARVPNSIVVGI
                     ANTDRKRDLTYPTTITEDKQHYPTTGGSAAFMSFIEKELQPFIETNYSITHDYTLIGQ
                     SLGGLFATEVLFTKPQLFNRYIIISPSLWWDDASLLKQHPVILDSTFTQQLDIYIGVG
                     KEGLAPCTIPHVMEVDANLLSEMITYGSAPHVSVHFDYLPEEDHATVTHQAVFNAFRI
                     LYPEPKEE"
     gene            complement(10396..10977)
                     /locus_tag="PLM90_02165"
     CDS             complement(10396..10977)
                     /locus_tag="PLM90_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HMZ88200.1"
                     /translation="MQHNNSLNTIIRSRRSVKPEQYNTTPIAESIILEMLENARWAPT
                     HARTEPWRFVVCTGDGLKRFAEYQAEQYKLHTPAESFNANKYDKLKTTPLKASHIILV
                     GMKRQETEKLPIEEEIAAVACAVQNMLLTATANGIGAYWSTGGITYHQAMKNYMGLGE
                     NDKALGMIYIGNYDIELPEGVRSGIEDKVRWEQ"
     gene            10997..12079
                     /locus_tag="PLM90_02170"
     CDS             10997..12079
                     /locus_tag="PLM90_02170"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HMZ88201.1"
                     /translation="MLRNYYIYTIGLLFSAIIFSCKGEKSGVSDDKQDPDQPTEKKVY
                     DRWLIDLEMVESGCERLDCIRSIDEPKFIPVDKVNFISDEDLVIGVKIGDEIHCYPHN
                     ILNWHEIVNDNIEGKYIAVNYCPLTGSGMAWNRNINGKLTTFGVSGLLYNSNVMPYDR
                     TTRSAWSQMKQLCVNGELADKHIESFPVIETTWSTWKKLYPNSKVVSTETGMQRDYLE
                     YPYGDYRENRDIYFEVELENDTLHPKERLYGVIQGDKAKCYRFDNFKNGIRILGDTVF
                     EKPIIVVGSERENFIIAFENPENLSFKPYTKQIPAVIQDNAGNTYDLFGTCIEGPKKG
                     TSLTAMNGFTAYWFAWYAFYPDVKFE"
     gene            complement(12085..12621)
                     /locus_tag="PLM90_02175"
     CDS             complement(12085..12621)
                     /locus_tag="PLM90_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88202.1"
                     /translation="MNKIVTIAAVAVLLSLSSCTAYLSPYTSTTQSQTNFDETQLKKV
                     QFYLSGDITLYRELAASETQITGGDIKIVDGKKVEQFVIKSGTPGVIIKAEDRDTFLV
                     SFDADGSFLRFGANNDYNGKYTLMAKEWQGRVGIVDYAGREFKSTPESIYAYLAVNME
                     KVNNTTVNTKEAGGRTIE"
     gene            complement(12673..14256)
                     /locus_tag="PLM90_02180"
     CDS             complement(12673..14256)
                     /locus_tag="PLM90_02180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ88203.1"
                     /translation="MATSSRSPLIKALQRAFATSLYSKKHGVPLGEAHEMVQQQFSRR
                     KFITTTSTAAAITAISGSAFLEACTGKRAPVVAIVGGGIAGLYAAYILRNAGIIANIY
                     EASGRTGGRIYTMKNLMGKGTHTELGGEFIDSTHADILRLADDFNLGLIDTQVESETS
                     LKQSLFFIEGQPVDDATLLNMLAPFVPAIQQDVASLSADISYTTHTDADIALDNMSLQ
                     AYLQHIGITGPLYKLIDVAYTTEYGLDVAQQSAINFLMLIDTSMKDRFRYSGFSDERY
                     KIAEGNQSITESLTDLLEGQISLGMELIAIGQKNEGDKVLLQFSETGGKHSTVEADYV
                     ILTLPFTILRNVDITYPITANKRRAIQELGYGQNAKFFMQFQKRVWREQGYTGFMFSD
                     SIVQNAWDHTQLQPGETGGFTVFTGGAASKVMETTKMPDFAAMCMNVLSKAYPHISDP
                     NPDMLTRFHWPTHPYTKASYTCFAPGQFTTIEGAQREPAGRLYFAGEHCSFEFQGFMN
                     GAAQTGREAAEAVIKALKG"
     gene            complement(14297..15754)
                     /locus_tag="PLM90_02185"
     CDS             complement(14297..15754)
                     /locus_tag="PLM90_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ88204.1"
                     /translation="MKKALILVSFVLSLALAGAQTAYTLESIPKPFPPTSYVSNPDGV
                     LSSTVEEEINTVLRRLEDSTTAQVAVVIVNSIGSEVPGDFKTELFREWGIGQSENDNG
                     LLILLVMDQRRMEFETGYGLEGVLPDILCKHIQETYMVPHAKEGDMDNAVLSGVMQIY
                     QILTNPAYHDEIYAGSLENDANFPWWRRTTSNGLLIGFAAVYFLFAIIGYRGGMKHEK
                     SAPAYVKSQYNSKYATAKFGLLNMALPLGFYAWQELAGNLRVFEFALAAYGLLMILLM
                     EKRLRLNHFIHRDTENKLPQDTYNLLSRSHSNGWIMADIFFPIPFIFYSLINKSRMKA
                     MRNIPPLADDGTLMVKLDEKADDEFLKTYQIAEENIRSVDYDVWRKPNSNEIHIFRFE
                     NYYSKYKECPNCKSKAYLMTKNKTIVSPTYDSTGQGEKTYNCKACGHERKERYTIAKL
                     THSSSSGSGGGFGGGGGGGGFGGGSSGGGGGGSSW"
     gene            complement(15777..17357)
                     /locus_tag="PLM90_02190"
     CDS             complement(15777..17357)
                     /locus_tag="PLM90_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMZ88205.1"
                     /translation="MMKLHLLLPAICIIAATSTFAQSQDLGVKYANTITAEELEQHLS
                     VIASDYYEGRETGTRGLARTADYLSKQFQQIGIPPLRPIGGYYQEYPLIEFGWNASTI
                     STEKAVFNMMQDFIGYASANTSMAISDNQIIFLGYGIDDSLYSDYRGVDVKNKIVIVA
                     NGEPKLNGISRVNNKDSVTAWSKDWRKKAEAATRNGVKCLLVIEPKVPEYLNNPSWRR
                     FLEGTLMKLESEYKQPEYNNNFFITPAMAESLLGKRTKLMKKSLQSIDDTGLPRNFAI
                     HKTVVFNINKLENKIYADNVIAFVQGTDLKDQVIVVSAHYDHLGMRDSVVFNGADDDG
                     SGTVALLEIAEALAEAKKAGEGPRRSVMFVAFSGEEKGLLGSKAYAENPVVKFEYTVA
                     DLNIDMIGRVDDAHLADPNYIYIIGSDFLSSELHNINEAAAKNYSNIKLDYKYNSTTD
                     PNRYYFRSDHYNFAKNNIPCIFYFSGVHADYHQPTDDIEKIDFPLMTERARLVFHTLW
                     LLANQDARIKVDRTPPQE"
     gene            complement(17357..17797)
                     /gene="rpiB"
                     /locus_tag="PLM90_02195"
     CDS             complement(17357..17797)
                     /gene="rpiB"
                     /locus_tag="PLM90_02195"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HMZ88206.1"
                     /translation="MSTNIIAIGCDHAGFPYKEGIIKYLESRSYEVHDFGTHSADSVD
                     YPDHVHPVASEVETGIASFGVLICGSGNGVCMTANKHLGIRAALVWQSDIAALARQHN
                     NANVICIPARFVTLEDAIAFVDVFLHTQFEGGRHANRVTKMTDF"
     gene            complement(17867..18697)
                     /gene="tatC"
                     /locus_tag="PLM90_02200"
     CDS             complement(17867..18697)
                     /gene="tatC"
                     /locus_tag="PLM90_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMZ88207.1"
                     /translation="MAAIKKNPDEMSFMEHLEALRWHIVRIAVVIFLFSIAFLIYPQF
                     IFDKIILAPKSPDFITYRIFCNIGERWNIDGICVDNLDYDLFYLNMTSGFLFYIKIAF
                     MAGIIFAVPYILWEIWRFVRPALHDKEVSNLGGIIIIATLLFYVGALFGYFLMAPFSI
                     NFLATFSLSDQIQNQFTFDSYLGILTGMVLWTGVIFEIPMVAYFLAKLGLLGREFLAK
                     NRKYAAVISLILAAVITPSSDAFSLFLVALPLWGLYEVSIVVVNVVEKRRRKRELAGN
                     "
     gene            complement(18716..19714)
                     /locus_tag="PLM90_02205"
     CDS             complement(18716..19714)
                     /locus_tag="PLM90_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88208.1"
                     /translation="MKKLSIIALSALAFSACVPQRQYQDAITARDACMQDTAVLHSQL
                     RMKTKEVDELSQQLDEVYASINLMRKDSIDIHNRYDAMVKANEQLREIEDVLNNRINQ
                     LLALSANENQQLREDLQKKSDELIAKEKELNAKAMTLSDQQKSIDALTADLEARSKRV
                     EELESILNKQEQTVTDLKNTIDKALSGFSASDLTVERKNGKVYVSLSEKLLFASGSTV
                     VDPKGKDALKKLADVLKANPDIRVEIEGHTDNVPLKSNNFPKDNWDLSVLRATSIVKI
                     LTQENGVDAKRVIAEGRGEFFPIADNTTTEGKARNRRTEIILSPNLDALYDLINSK"
     gene            complement(19774..21318)
                     /locus_tag="PLM90_02210"
     CDS             complement(19774..21318)
                     /locus_tag="PLM90_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HMZ88209.1"
                     /translation="MDRNEQFKQVIQEGYNFQGTSIVLGTAILDRQPMPQTHVKVPLR
                     MMNRHGLVAGATGTGKTKTLQGIVEQLSDNGVGVLIMDIKGDVSGIAMPGTGNPKIDE
                     RMQQIGKSWQAKGYPVELMSISNENGVRLRATVSEFGPVLFSKILELNDIQEGVVSLV
                     FKYCDDNKIPLLDLKDFRKTLNYLTSEGKKDISADYGTISTSSAGTILRKVIEIEEQG
                     ADLFFGEKSFDVEDLCRMNDRGQAYVNILRLTDIQSKPKLFSTFMLCLLAEVYNTFPE
                     IGDKEDPKLVIIIDEAHLIFKEASKSLLEQIQTIVKLIRSKGVGIFFCTQLPTDIPAE
                     VLSQLGLKVQHALRAFTANDRKAIKLTAENYPITDFYQTEDLLTNLGIGEAIITALNE
                     KGAPAPLAHTLLVAPQSRMDVLSAEEMQQLNNISSLTKKYNESVDRESAYEILNKKLA
                     ASEQAAEREKQLKEEEAKQKLEERERKVKKTKEKPLIDKTTQHQITRTVINVLERGLL
                     GMLKRR"
     gene            21374..22747
                     /gene="radA"
                     /locus_tag="PLM90_02215"
     CDS             21374..22747
                     /gene="radA"
                     /locus_tag="PLM90_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMZ88210.1"
                     /translation="MANKAKTAFFCRNCGFESSKWLGKCPSCNEWNTFTEEVIERPKD
                     AASWKDSKPEKRISGPVQLSEITASDTGRLVTGDLELNRTLGGGIVAGSLVLIGGEPG
                     IGKSTLLLQLALELKQIKVLYVSGEESEQQIKMRADRLKGNNQGLYISTEVAISKLFM
                     QIKQLQPEMVVIDSIQTLVSDFVDATAGSIAQIKECAGQLQRFAKETNVPVFIIGHIN
                     KEGYIAGPKILEHMVDTVLQFEGDRNYTHRILRTIKNRFGSTSEIGIYKMEGDGLQQV
                     NNPSELLITEKEEPLSGVAVAATMEGMRPMLIEVQALVSPAVYGNPQRTATGFDLRRL
                     NMLLAVLEKRGGFAYGNKDVFLNIAGGLRVEDPAIDLSIACALLSSLTDMALPLNMCF
                     AAEIGLSGELRGVARIEQRITEAEKLGFDRIYIAKHNMKSLKQNKYNIELRAAGKLEE
                     VYGQLFV"
     gene            22890..23588
                     /locus_tag="PLM90_02220"
     CDS             22890..23588
                     /locus_tag="PLM90_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HMZ88211.1"
                     /translation="MFPLLLLNELSDMFYPRLCTACKEPLLAHEHIICLRCRIELPRT
                     DHGLFHDNPVAVKFWGKVPIEEAAAMLHFHKGSRVQSLMHALKYKNRTDVGITLGNML
                     GYYCKQYHLFKQITAITAVPLHKERLAVRGYNQSAVIGEGLAEVLQVPFKGEWLERIT
                     PTATQTKKSRLARWDNVKDVFRVTDPDGIAYQHILLIDDVITTGSTLEACALKLIQSV
                     GVKVSIAAIAFADR"
     gene            23965..24927
                     /locus_tag="PLM90_02225"
     CDS             23965..24927
                     /locus_tag="PLM90_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ88212.1"
                     /translation="MNKTILFDFNVDKEARTIRVDRSFDAPRDLVWAAWTESDILDQW
                     WAPKPYRNVTLSMNFVEGGRWHYYMISPKDEKHYCLFDYHRIIPETMYSGMDAFCDID
                     GNISESMPRMQWENSFEKQGDTTVVHVLINFNKIEDLEAVISMGFKEGFSMGMENLDA
                     YIASQFYLRKSKKTSNAARVCTYLNFPGNTEDAMLFYKGIFKTEFVQGIQRFGDVPSD
                     AGAPPVPDALKQMVLHVELPLIGGHILMATDAPQEMGFEVKVGNNMHISLEPDSKDET
                     TRIFNALAEGGNITMELQDMFWGAYYGSCTDKYGINWMLNFQQH"
     gene            complement(24982..26172)
                     /gene="hflX"
                     /locus_tag="PLM90_02230"
     CDS             complement(24982..26172)
                     /gene="hflX"
                     /locus_tag="PLM90_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMZ88213.1"
                     /translation="MAHKPTIKPNETETCILVGLVTKDQTLEQLNEFLDELEFLAETA
                     GAVTKAKFYQKLPHPDTKSYVGKGKLFEIMDYVVANEIDIVIFDDEISPSQLRNIEET
                     LKVKVLDRSMLILDIFATRAQTVQAKTQVELAQTQYMLPRLKGLWGHLDRIKGGIGMR
                     GAGEKEIETDRRVAQQRIALLKQQLEKIGLQNETQRKNRGELIRVALVGYTNVGKSTL
                     MNLLSKSEVFAENKLFATLDTTVRKVVLDRTPFLLSDTVGFIRKLPHHLIESFKSTLD
                     EVRESDILLHVVDVSHPAFEEHIHVVNETLKELEAIDKPTILIFNKMDAYEERYFDKY
                     LTPDVKRDILSELENTWMQRLNAQCVFISAINRTNIELLRSMLTDMVKEQYQIRYPYK
                     TEFF"
     gene            26289..27176
                     /locus_tag="PLM90_02235"
     CDS             26289..27176
                     /locus_tag="PLM90_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88214.1"
                     /translation="MQINFLRHNEIDRKNWDRCIDNSLNALPYAYSWYLDIVSNGQWD
                     AIIIDDYAAVFPIPFKNRLIYKQVYQPFFTQQLGLFYTDKLYHTKLPDVLKALPASYR
                     KIHLHLNIENDLGEVFSAVEKRVTHHIDLNKSYQSIASDYNEQIRRNLKKSLTHHLQV
                     ETSSDVNILISLREMFLQEALKGKQSAADTDRLKKLIQAILDNKKGEIKFVRGAENDI
                     QAAVCLLQSNGYIIYLSSVSSDVGKANHAMTFLIDDMLKSNANTQQTFDFEGSMIPGI
                     ARYFKGFGGKEKWFSVIQK"
     gene            complement(27189..28463)
                     /gene="glyA"
                     /locus_tag="PLM90_02240"
     CDS             complement(27189..28463)
                     /gene="glyA"
                     /locus_tag="PLM90_02240"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMZ88215.1"
                     /translation="MRRDTEVFDIIKKELHRQTEGIELIASENYVSDQVLEAMGSCMT
                     NKYAEGYPGKRYYGGCEYVDVTENIAIDRAKKLFGAEYANVQPHSGAQANAAVMLACL
                     NPGDTLLGFNLSHGGHLTHGSPVNFSGKLYRPVFYSVEEETGRIDMDKVEATAIAEKP
                     KLIICGASAYARDWDYARFRAIADKVGAILLADISHPAGLIAAKLLNDPMPHCHIVTT
                     TTHKTLRGPRGGMIMIGKDFPNPWGQTTPKGEVKMMSAVIDSAVFPGTQGGPLEHVIA
                     AKAVAFGEDLDEGYVAYCKQVIANAQAMANAFVSKGYKIISGGTDNHLMLIDLRNKNI
                     SGKKAEGLLVDAHITVNKNMVPFDDKSPFVTSGIRIGTPAVTTRGMKENDMVRIVEWI
                     DRLIMAPDDDAVKTAVRNEVNAFASQFPLYAY"
     gene            complement(28788..31556)
                     /locus_tag="PLM90_02245"
     CDS             complement(28788..31556)
                     /locus_tag="PLM90_02245"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ88216.1"
                     /translation="MLKKLLLSGSVFGLTILGLSGQVVINEYSAANYSDIADNYGEYE
                     DWIELYNAGATAVDLSGYHLSDRIGSPTKYTIPPGVNISAGGYLRFWCSNRNTAVGTN
                     YHTNFKLTQTTGAEDVVFADAAGTVLDTRELNNPNQTNHSWGRYPNGGADWKIFTDPT
                     PNASNSTTPKTAYAHKPDMEPNSGNFAGSVTVTITAPEPGMTIRYTTNGNLPTAASTA
                     YTGPITITETTVLRTVAFAASADTLASFCSTNTYFIDETTTMPVISVAGDQVDDLLNA
                     TGWGIEPVGSFELFDKDFNLIDEAVGDFNEHGNDSWAYDQRGFDFIVRDQFGYDNDID
                     QTFFNDVTDREGYQRLIVKAAANDNYPSSSGGAHVRDAFVHHISGLDGLELDERSTFF
                     NIMFLNGDYWGVYDTREKADDADYTDYYYGQGEYEMDYIKCWGGTWAEYGTMAPWSPF
                     VTFVTTNDMTIPANYDYVKDNFNTMSLIDYVLTNTFSVCTDWLNWNTAWWRGYDATGS
                     NLTWRYALWDEDATFGHYINYTGVPDDSPTASPCDPLTLGGVDPNGHIDMLNSLLDNP
                     DFHSDYINRFADLINTTFSCEVLLPRIDSMRAVLDPEMTRHTDKWGGTYTGWSNNYDE
                     LHDFIAERCAYLPSGVEDCFDVTAYGIVVKIEPAGSPNDVRVSTIIPDTYPYSATYFS
                     GTTLGFQAIADPAFTFDHWEFINHVPSPSTTDDSVTVSLTTTDTIIAWFAGSELPMYD
                     FTLDIDPPGAGVATVEGFSPAAYPYAGAYESGTFIDLSATPTGSYVLDYWSLENHTAN
                     PDPFAPDIFFALSDIENAVAHFRLKTSVEENGVSVSMTAVPSVTAGPVQVHYGLSAAQ
                     DNMQLTIYSLTGQLIADLGADQLISGAQGEHIVSVDLNKYHLANGMYFIRLTAGNQSI
                     NERVMLAK"
     gene            31748..32752
                     /locus_tag="PLM90_02250"
     CDS             31748..32752
                     /locus_tag="PLM90_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ88217.1"
                     /translation="MKVIIPVAGVGTRLRPHTYSQPKPLIPVAGKPILGFIIDRMVKA
                     GFTEFIFVIGYLGEKIESYVLEHYPELKCRFVIQNKREGLGHAIFLTRPLISENEELY
                     IVLGDTIFEADIEAVMTMPESALGLKRVDDPRDFGVAEVEDDMRITRVIEKPSIPKSN
                     LALVGLYKIKESAQLFNALEQIMAHGYKTQGEYHLTDALMMMIKDGITFRGFKITNWY
                     DCGKKEILLETNAILLKKLHPQGQHQHAYPNTILIEPVSIAPGCDIRDSIIGPNVTLG
                     ENTSVHYSILRHSIIGNDATIDDVVLLDSVIGSDTVIKGMSQSLNIGDNTEIDLSKSG
                     "
BASE COUNT         9729 a         6939 c         6642 g         9513 t
ORIGIN      
        1 atgtcaagtg ctgtcaatac gctcaccgac aatgcatgga gtaaccttgt atccgaatcc
       61 ggttgatgac attctgatga tcgcaggaac aatagacaat atagtttcgg tctctgtggt
      121 gaatacgatt ggagtagaat tattttgtca acaaagctgt acagatttaa attatgtact
      181 aaccaccgca tcattggcag cgggaaatta ttattgcatt tgcaaaagcg acatcggcac
      241 ccgaattatc ttaccgttta ttgtggaaca ttgattgtaa ctttacagga aagtacattt
      301 catgagattg acttatcgcc tgagtgagca agatttttta aactgtcatt tattctttac
      361 atcaagatca gaagctacaa gaaaagcgag gaaacgcaat cgcctgctta tgcccggaat
      421 ttatatcgtt ttgggccttg ccaccttatt tatcggcgat cctattttaa gtggattatt
      481 gatcgtagcg ggtgtattat ggtatttttt ctttccgggc tatgaaggca aaagaatcgc
      541 tacacgcatg cagaagcatt tgcaagaaaa cttacgatct caattcgatc aggagaccaa
      601 cctcgacatc accaaagaaa tgattattga taaggttaat acgttggaat ccagaattcc
      661 ggcaagtgag atcaaagaga tttacaaagt gcccggatat tattacttcc tgactaaaac
      721 aggaacagca atcatcatac cacgaaatat cctggaggat gaatcaacat tgaatggggt
      781 gatacaagag atgtgtgatg atttaaaatt gccctaccgg gaatttgccg aatggaaatg
      841 gaaataaatc aattcagttt ttaataaatg attttgtgtt ccttacacca tcaacagaaa
      901 tactgcatgt ataaataccg gcaggtagat tttctaatgt gttgatctga aaaacgtccg
      961 ttcccttctc aagatattca tcgcccttca agcacactgc gcctaatgca ttacaaatct
     1021 gccactttat tgatgtagca taatcattag tataacctac attcaagata ctttgcacag
     1081 gatcaggaaa tattgaaaaa gataaattct gctgcaactc attgatgtca tctaatagta
     1141 caacaagtag tgctgcattg ctggtatcgg agcagatgtc gtacgcaaca atacatcgga
     1201 actgaaggcc gtcataccag taatcaacag gcgcaataga cagttcggag gtatgggttc
     1261 ctgaatacgg atagacatct tcaagcgatg tccatgctac accatcccat tcctgccact
     1321 gatacattgc atccgggaag ggtgaagtca cattgaacac tgccgtagcc agcggaagaa
     1381 taataacgtc cattggctgt tcagtaatgg tatcgcaagt gatatatggc gaattaaaat
     1441 acctcagcac ggcaaaatcg ttatcaccgg catcgtttgt aaatcctccg gcaagaatct
     1501 tagtatcatc ctgaattaaa acatcataag ccagatccca atcatcaccc aagtgagtga
     1561 ttacgatacc atcatcgctg aaagtgctat caggtgatcc gtcgggtaat aatttcgcca
     1621 ccaaataatc atagccgtcg gcataggttc caatctcacc gccaataatt atcctcccat
     1681 caggttcgta tgcaatacca aatatttgct cctcttgata cggatctacg ttgatcacaa
     1741 ctcgtccgcc atcgccaaat gtagtatcca attcaccatt tgtaagatat cgggccattg
     1801 caatattttg atcgggcaca acatctgtag taccggcaat taccaattta ccatcaggtt
     1861 gcagcatacc acaatatgcg aaattcacag tgctgtcaaa atcagttctt actatgccat
     1921 catcaccgaa gctgacatca agggctccat ttgaattaaa ctgcatgacc gtcatatcaa
     1981 aactattcat aaggtcttgt gtatatccga caagcattat tctatcatca ggacgcagga
     2041 tcaacgctct tgcctgatcc catctaccaa atactgtttc agttgttttt ccatccactc
     2101 caaatgagag atccggatcg ccattttcat aaaaccttgc taatgcaaat cgcggataaa
     2161 atccatcaac agttgcaaaa ccaccaacaa ctatcttgcc gtctgattgg gtggcaatgc
     2221 cgaaagcttg atcattttca agatcaaaat cgaccagcaa cttaccatca ttactgaaag
     2281 tagtgtctaa ttcaccatca ggcagatacc tcgcaattgc ccaatcgttt ccactatacc
     2341 ctgctgccag aattcgtcca tcgggcatga gatacatggc attggcaaga tcatgagcca
     2401 attcgaaatc agtagttaca ataccgccat cgccgaagct ttcatccaag gatccatcag
     2461 ttaggtaacg caacaacaca aaatccgttt ctgtgcctgc aatgaaatta gtaccaccgg
     2521 cacctaatat ctttccatca ggttgtaatt ccatagcaaa aatgacatca tatccatcgg
     2581 caaaagcagt ggttacgata ccatcgccat caaatgtagg atccaagtca cccggaaatt
     2641 gcgcaaaaat gcaagctcta aagaacgtca gtgattgtaa aaggagtaaa gatctcagaa
     2701 gcatacggca acctttatat caaatatacc acatttgttc gtttagattt tgcatcgcca
     2761 attttgatta cactattttt atagcacaaa ggctcctaca tgcaatttac ctatcgcaac
     2821 ggcattctta aaaagtattc catcctcacg cttgttttat caatgacatg catttcaatg
     2881 aacggacaag gcaatggaga ggctcacaga cctttgatac attttacgcc ggaaacaggt
     2941 tggatgaatg acccgaacgg attaatttat tacgatggat attatcatat gttttatcaa
     3001 tattatcctg attcaaccgt atggggacca atgaattggg ggcatgcgca aagtcttgat
     3061 ctgatgacat gggaacgaca gccgattgcg ctttatcccg attccctggg ctggatattt
     3121 tcgggaagtg ccgtcatcga tgtgcacaat actgcaggct ttggcaataa tgccatggtt
     3181 gccattttca cttatcacaa tgatgccatt tggcaaagcg gaaataaaaa caccgaaagt
     3241 cagggattgg catacagcac cgacaaaggt gccacttgga aaaaatatgt aggcaatcct
     3301 gttttaaata ataccggcga acaagatttc agagatccga aagtattctg gaattcatca
     3361 atcagaaaat ggaatatggt gttggctgtt ggcgaccata ttcgcatatt ttcatcccct
     3421 gatttaaaaa aatggacatt tgaaagtgat tttaaaccgg aagaagatga ggaggttggt
     3481 gtttgggaat gccctgattt atttcaaatg gcagtagcag gaagcagtga taaaaaatgg
     3541 gtgatgctta tgagtcatgg ttacaacgaa aaagacaaca aacaatcagg gacgcgctat
     3601 tttgtcggcg atttcgatgg gcatacattt aaaaacacac aacaagcaca atggatagat
     3661 gtaggctcag atttttacgc tgctgcaacc tacgctaatg cgccagggaa taaacgtatt
     3721 cttcaagcct ggatgagcaa ttggatgtac gcgcaggaag tgcctgcaac aagatggaga
     3781 agcactatga cattgcctag ggaattagcc ctaattaaag ataagtatgt atacgccatc
     3841 acgcagcaat gggctactca aataataaac aatcaaagtg aaatatacag taaacctgaa
     3901 tcttcagatg ttgaattatc agggaatgta attccgcatt gccgaataac cttagacctc
     3961 gaagaaaaca ataccacttc catacagctg tcaaacaatg aaaacgaggt gtttaccatt
     4021 ttgatagacg gcacaaccat cacaacaaca cgtgatgttt accgcgacga ggatgctatt
     4081 tatcctttta ttacagaaac tgcagcgagc ttacccggag agcgcatcac caaattggat
     4141 atgatattcg atgtcggttc ctgtgaaata ttagtgaatg acggaaaatt atccatgacc
     4201 accatttatt ttccgacaag taactttgac aagctatcca ttaatccgat ggatggagga
     4261 attttaagaa acgtgacaat ttatggtatc acgccaacaa tgtggtaaat tatgatgcat
     4321 agccctgaaa ttccaacaga ccgtcaaaca tgggagagtc caattgctta ttattggtgg
     4381 gaggatgaga tattgatatc gctgtcaaaa tctacattga ggacagttga aacgatcaaa
     4441 gccaatgttg caatagttca aaccattacc ggaaacaagc ccacgccctt gctgatatat
     4501 ttatcaaaat cgccgattcc ggataaagaa actcagtcat attccaaaac gcaattacct
     4561 ctcatttatt cttccatggc aatggtatcc aaaccgggac tagcacaatt catcatgcgt
     4621 ttactgttca gttttcaaaa accgccaatc cccatgaaga gttttgccga tgccgaaaaa
     4681 gcaaaacgct ggcttcgcag tcattaatta ttctttaata aatgcctgtt gtagttgaga
     4741 cgtgtcgtta atccactgaa ttaaatacac acccggcaaa taatttgaaa tatctaattg
     4801 catggtcgaa gcagaaacat caaatgtctc tttaagtgtt ccggccagat cataaacatg
     4861 catcaaacca tcagtttgca aattgtcggg cagcgatata tttatttgtg cattcgccgg
     4921 atttgggaat attaaaatat caccttcaag ataatttgca atcgattcta caatagtcgt
     4981 gtcctcacct gaagtatcgg tagtagtatc ttcagggacc agacaaggaa aataataatc
     5041 catacccggt gaaccttcag gacagctctc catccaattt gaagcaaggt tgatattctc
     5101 caacacatct acaatttgca atgtcacgcc tccaccatca ggggaaattg gatatggtat
     5161 cgtatcatca taactaacgc tctgataaat cacgccacct gcatcataaa tactgatgat
     5221 atcatcatca ctgctgagtc caaaccctgt aggcccggaa atattagtca catcaggaaa
     5281 gcgcgatgcg aaaagggctg catcctgata aattacaata aaactgtcgg gcaaaatttg
     5341 ggtacctgtc ggtaacacat atgcatttcc atttttatcc tttacggtcc aattaccaat
     5401 atcaattata tccgcacttg tattcattaa ttcaaaccaa tcgcccgcat ttgaatcagc
     5461 atcagaatta tagttgatct catcaaccag tggattttcg agacatgatg taaaagctgc
     5521 agccggcgaa ccacctacgc aacccggaaa ccaggcttca ggtaaaccgg gatctattgc
     5581 aggttcaaag cgttcaagcg ttcgcccgta attatcagca gttacaggcc aaggtcgctt
     5641 atcattaaat ttgaatgatg tgataacatt accaagaaaa tcgattagcg taatggaatc
     5701 tccatcattg gcaagattga aatcaaatcc gccaataacc tgtgtgacat caggataaac
     5761 ggcattgaat tcaatcatat cttccgccag cactaaatat ccatcaggcg cgattttaat
     5821 ggaaggaaat atataatcat tgtagaaaat ggtattggag aattgataat tagtcaaatc
     5881 aatttccatc ccggaagtat tatgcaactc aatccaatcg ccggcattta atgtgctgtc
     5941 agaattataa ttgatttcag aaataatgat gggattggaa atacctgtgc cattaaaatt
     6001 ggcagtaaat gttgttgcct ctgtaatatt cacattaaat gcaggcacat ccgctgttgc
     6061 aataaatgga ttatccgtcc agttaacaaa agtatagccc gggttggcga tagcagtaat
     6121 cgttacagga acaccatcaa aatacacacc tgtccaaggc aaagtttcag gaataattgt
     6181 cgatattttg atatatccgg ctccggcggg tgctacatca atagtcacat ctacctgatc
     6241 attaaggtca taataatttt ctatatcatc tcgcacataa ccacgtctgt cgttatgaaa
     6301 attgcgcaga ttagtgattt tgctttgcac aaaactcgaa tcgccggtat tccatttgtc
     6361 gtcctgtgca ttcatctcgg tgattatttc ggctgtattc gcatcgatca atgcattggc
     6421 aatttcgggg gtatagtaat aattcaacaa atcggcaaag cgattaataa agtgatcatg
     6481 aaattcggtg ttctccatca acgcattgaa catatcaata taaaaattat tgtcatccat
     6541 agccgttgca agtgtatttg aacttgctcc ggcaccgggt gtaccttgcg atgatccaag
     6601 gtcccacaac ataaatttcc attcaatatc cggcgatacc gctctgaata aacgtatatt
     6661 atttacaata aatccaatcc aatcggagtt agcgctatac acttcagttg caaaataatc
     6721 tatccagtta tcttcatcaa tctgatccaa tactgcagca taataaaccg gatctgtcag
     6781 atcatgatcg atacaataag tatgcagatt aaaaaaacct gtatcgcttc ctgcctctgt
     6841 aacataattt ccaccgccac taaagtgagc ctttatcatg tctaccctat cagggtttgc
     6901 accgaaatta tcgcgacaga aatatgggtc taaacgctct cgagaaacat aatgacccca
     6961 atattcgccg ttgataaatg ctgccacatt ttggtttgca gctgcaacag tatgcgttgg
     7021 taatgccatc ctgttaatca gcaagtcgcg cgaacgagcc aactctgcgt cattatcgca
     7081 attgcgcaag cgaaatgcgt gatatttatc aattacgggt ttatccaggc tgtacaatgg
     7141 gaaattgata ttatcggggc tattaaaatc ctcatcacaa gtaaactgaa ttcctttctg
     7201 atccaattca atagaataat tccctgccac ttcaaatttc attggtgcag cgaattgaaa
     7261 tgttttatcg gcattaaagt attcgataac acaattgatc tggggatttt cattgtcgaa
     7321 tgatgtagca ttatcatacg ctgcaggaca ggtggcggca agatcaaaca aatcgcacgg
     7381 tgtggcactg atacttacta caggtaataa tgttggctca tttattaaca gactggtggt
     7441 tgccagcgca cttggcaatt tccctgtttc aaaagatctt gctttgataa cagtagatgt
     7501 agtaacaaca aacggatcga tataggaaat acctcctcct aataaaggat cgcttccatc
     7561 agttgtatat tgcacatcaa cccctgaaat ggtaaccgtt tgatcgccgg tatagaatcc
     7621 gggtaatggt gtaatggatg gcgtggcagc atatagcaaa tagcaatcac cggaattcac
     7681 ggtgtcggga gtcggtatgt ttgtcataca ccagccacct gcatcaggaa ttcttgcttt
     7741 tacaattcct gcttctaaat cttgcatggt aatgctatca cgcaaaatac ctgcgttatc
     7801 gaataacgca atagtttcac caagtcctat tttgaaattt gtatgtaatt cggttgaatt
     7861 cgttgggtta aaccacagag gcggaattcc ataaaacaca gaagcatctg tgatagcgaa
     7921 atgcaaaaat gttctccctg tgaggtcatt tgagcctgat gttttattat gcacttggac
     7981 agcgataaca ttcgtaccat caacaataat tccggctagt gttgctgaat caagcgtaat
     8041 atatgcagga ttaccaccat caaacatttg agcttcatgt gcagtaaccg acaagtcatc
     8101 ataagcaggt attcccgtta ggccggcttc actaattaaa atgccattaa taaataccac
     8161 ataaccatcg tcataatcca tattgataat ggcatgaatc aattttgttt tatcggcaac
     8221 ttcaaatgtt tttctataat aaaccgatct cacatcagtg gtaataatgg tactatcatc
     8281 atcatctcca aaaccaaatc caccttttcc ggtatcccaa ccaaagggtg cagttgaggt
     8341 acgccaagat ggatcgggct cagcatctgc agggaagtat tcccatatat catcatcata
     8401 cacagcagtc tcccaatgat cgacagaggc catttctgca tcgtaggcat tgcgattttt
     8461 ttcagacgcc atgatcagca gatgcgccgc cggtgcgaga acggtatcgg gaagctgcca
     8521 ttttccgggt atagctaaat catcggtaac cgaccacatg gccatgttca ccggatcaac
     8581 accaccattg tataattcaa tccaatcagg caattcgcca tcttcatcta ccacaatacc
     8641 ggtgttggta tgcgcagctt cgttaattaa tacttgtgcc tgaataagcg agaaaagccc
     8701 acaaagtggc aatacgataa gtcgtttcat ttggtgtgcc ctgggttgca attaggttag
     8761 ggtaatcaaa tatagaagta ttgcggcggt aatccaaact ttaacaattc ggacatcatt
     8821 cgaattattt caatatctat tatgttaatt tgtagaatca accacaatcc gctatgtcaa
     8881 aaccattcgc cacaaatccg gagttggtgc ttcgtgaaga acttgccata caacgaacaa
     8941 cccttgcaaa ccaatcaact tttctttctt ttataagaac cggattgtat tttttgttag
     9001 gtggattgag ccttacaaat ttgttaaaaa tgaatgacgc agcactgatg gaagtaatca
     9061 tgttttcaat tgctgcctta ttagtcatta ttggaagctt taattatatg catcatcaac
     9121 gcaaaattgc cagaagtaaa ttgcaaatag gtcaagtagc gcaagcaaca aggagttaaa
     9181 atatagatat ttattatctt tgaaattatg ccaaagcgca aattatacac ttgctctaag
     9241 ggtcatcaat tttacaagag cagcgattgt cgctcgtgtc ctacatgcgc atcacttgcc
     9301 aagccaaagg atggattatt atctttatta tctgcaccgg caagaagagc attattacaa
     9361 cacggagttg aaagtgagca tatgcttgtc aatttttcca gagaagaaat attaagttgg
     9421 catggcattg gtctttctgc gatggcgata ttagaccttc aaataaaaaa gttgaaattg
     9481 tcttacaaaa tttcaaagct ctaaaattat ttttatcgaa ttgccttgct tatgcatatg
     9541 aataaattac aattcctgtc aatacttctt cttgcaacat tttccggatt cgcacaatct
     9601 ccttccgccc ggccatttac aattggcgtt actgaaatcc tccattctaa tattttaaat
     9661 gaggatcgta tcattaatat ttatcttccc gaaggatata gcaaagatga ctctattaat
     9721 taccctgtaa tttatttact ggatggcggt gcagatgaag atttcataca tatagccgga
     9781 cttgtccaat tcaacaattt tccatggata gcacgcgtcc ctaactcaat tgttgttggc
     9841 attgccaata ccgaccgcaa acgcgatctc acctacccta ccaccataac tgaggacaaa
     9901 caacattacc caacaacagg cggctctgct gcattcatgt cattcattga aaaggaatta
     9961 caacctttca tcgaaacgaa ttattccatc acccatgact acacactcat aggacaatcg
    10021 ctaggaggtt tattcgcgac agaagtgctg ttcacaaaac cgcaattgtt caacaggtat
    10081 atcataataa gtccgagctt gtggtgggat gatgcttcat tgttgaaaca acatcctgtc
    10141 attctcgaca gcacctttac tcaacaattg gatatttata tcggtgtagg aaaagaaggg
    10201 cttgcaccgt gtactattcc gcacgtaatg gaggttgatg ctaacttatt atcggagatg
    10261 atcacttacg gatcagcacc gcatgtatca gttcattttg attacttgcc ggaagaggat
    10321 catgcaacgg tgacgcacca ggcagtgttt aatgcattca gaatattata tccggaacct
    10381 aaggaagaat aaatgttatt gctcccagcg caccttgtct tctattccgc tgcgcacgcc
    10441 ttccggtaat tctatatcgt aattgccgat ataaatcata ccgagcgctt tatcattctc
    10501 tcctaaaccc atgtagtttt tcatagcctg atgataggta attccaccgg tactccaata
    10561 tgcacctatg ccattagccg ttgctgtcag cagcatattt tgcacagcac aagctacagc
    10621 ggcaatctct tcttctatcg gcaatttctc agtttcctgg cgcttcatgc caaccaaaat
    10681 aatatgcgat gcctttaatg gtgtagtttt aagtttgtca tacttattag cattaaacga
    10741 ttccgccggg gtatgcaact tatattgctc agcctggtat tcggcaaaac gcttaagccc
    10801 gtctcccgtg caaaccacga aacgccaagg ctcagtgcgg gcatgcgttg gcgcccagcg
    10861 tgcattttcc agcatctcta aaatgatgga ctccgcgata ggagtagtgt tatactgttc
    10921 aggtttcaca gaacggcgac tgcgaatgat agtatttaac gagttgttgt gttgcatctt
    10981 gtaactttgt ggtctgatgt tgcgaaatta ttacatatac accataggcc tgcttttctc
    11041 tgctataata ttctcctgca aaggagaaaa atccggcgtt tccgatgata agcaggatcc
    11101 cgatcaacct acagagaaaa aagtatacga caggtggctg attgatttgg aaatggtgga
    11161 atcaggatgc gagcgtttgg attgtattcg gtctatcgac gaaccaaaat ttatacctgt
    11221 agataaagtt aattttatca gtgatgaaga cctggtaata ggtgtaaaaa ttggcgatga
    11281 gatacattgc tatccgcaca atattctcaa ctggcacgaa atcgtaaatg ataatataga
    11341 aggaaaatat atcgccgtga attattgtcc actcaccggc tccggtatgg cgtggaatag
    11401 aaatatcaat ggcaaactga caacctttgg tgtatccgga ttattataca atagcaatgt
    11461 aatgccttac gatcgcacca cgcgttctgc atggagtcag atgaaacagt tatgtgtgaa
    11521 cggcgagctt gccgataaac atatcgaatc attcccggtg atagaaacca cctggtcaac
    11581 gtggaaaaaa ctgtatccca acagcaaagt ggtatcaacc gagacaggta tgcagcgtga
    11641 ctatctggaa tatccctatg gcgactatcg tgaaaacaga gacatttatt ttgaggtgga
    11701 attagaaaac gacaccttac atccaaagga aagattgtat ggagtaattc aaggtgataa
    11761 agctaaatgc tatcgctttg ataatttcaa aaacggcatc aggattttgg gagataccgt
    11821 atttgaaaaa ccgatcattg tagttggcag cgagcgcgaa aactttatta ttgcatttga
    11881 aaatccggaa aatctaagtt tcaagcctta taccaaacag atccctgctg ttatacaaga
    11941 taatgcaggc aacacctatg atctattcgg cacttgtata gaaggaccta aaaaaggcac
    12001 atcattaact gcaatgaatg gatttacggc atactggttt gcctggtatg cgttttatcc
    12061 tgatgttaaa tttgaataag gaaattattc gatggttctg ccaccggctt ctttagtatt
    12121 caccgttgtg ttgttcacct tttccatatt cacagccagg taagcataaa tactttcagg
    12181 agtacttttg aattctctgc cggcataatc tacaatacct acacgacctt gccattcctt
    12241 agccatcaag gtgtattttc cattataatc gttgttggca ccaaagcgca ggaaactgcc
    12301 atcggcatca aagctcacca gaaaagtatc tctgtcttca gctttaatta taacaccggg
    12361 agttcctgat ttaatgacaa attgttctac tttttttcca tcaacgatct taatatcgcc
    12421 ccctgtgatt tgtgtttcac tggcggctaa ctcgcggtac aaagtaatat cgcccgacaa
    12481 ataaaactgc acttttttca attgcgtttc gtcgaaattt gtctgcgact gtgttgttga
    12541 ggtatatggg ctaagatatg ctgtgcatga agacaatgac agtaaaacgg ctactgccgc
    12601 gatggtaaca atcttattca tccggtggtg tttggctgtt aaattgcaaa atttatgcca
    12661 tttcggtcaa aatcaacctt ttagggcttt tatcactgct tcggcagcct ctctgcctgt
    12721 ttgagcagcg ccgttcatga aaccctgaaa ctcaaagctg cagtgctctc cggcgaaata
    12781 caatcgccct gccggctcac gttgtgcacc ttcaatagtg gtgaactgac cgggagcgaa
    12841 acaagtgtaa ctggcttttg tataagggtg agttggccag tgaaagcgcg tcaacatatc
    12901 aggatttggg tcgctaatgt gcggataagc tttcgataat acattcatgc acatggcggc
    12961 aaaatcgggc atttttgtgg tttccattac tttgcttgca gcgccacctg tgaatacggt
    13021 aaatccgcct gtttcaccgg gttgtaattg tgtatgatcc catgcgtttt gcactatcga
    13081 atcgctgaac ataaatccgg tatatccttg ttcccgccat acccgctttt gaaactgcat
    13141 gaaaaatttc gcattctgtc cgtatccaag ctcctgaatt gcacgccttt tatttgctgt
    13201 tatcggataa gtgatatcga cattacgcaa tatggtaaac ggcaaggtta aaataacata
    13261 atcagcttca acggtgctgt gtttccctcc ggtttcggaa aattgaagca acactttatc
    13321 gccttcattt ttctgcccga tagcaatcag ttccatgcca agtgaaattt ggccttccag
    13381 cagatctgtc aaactttcag ttatgctttg attaccttca gcaattttat atcgctcgtc
    13441 gctaaatccg gaatagcgga atctatcctt catagatgta tcgatcaaca ttaaaaaatt
    13501 gattgccgat tgttgagcga catcaagtcc gtattcagtg gtatatgcta catctatcaa
    13561 cttatataat ggcccggtta tacctatatg ctgaagataa gcctgcagcg acatgttatc
    13621 taatgcaata tcagcatcgg tatgtgtggt atatgaaatg tcggcactca atgatgcaac
    13681 atcctgttga attgccggaa caaaaggtgc aagcatattc aataaagttg catcatcaac
    13741 cggttgtcct tcaataaaaa ataaactctg tttaagtgaa gtttccgatt caacttgagt
    13801 atcaatcaat ccaagattaa aatcatctgc caggcgcaaa atatcggcat gcgtgctatc
    13861 aataaattca cctccaagtt ctgtatgtgt tccttttccc atgagatttt tcatggtgta
    13921 aatccggcct cctgttcttc ctgaggcttc gtaaatattt gctattattc cggcattgcg
    13981 caaaatatat gcggcatata agccagcaat accacctcca acaatcgcca caaccggtgc
    14041 gcgttttccc gtacaagctt ccagaaatgc tgacccgctg attgcagtaa ttgcggctgc
    14101 tgtagaggta gtggtaataa attttcttct gctgaattgt tgctgcacca tctcatgcgc
    14161 ttcgcccaaa ggaacaccat gttttttact gtacaaggat gttgcaaaag cacgttgtag
    14221 tgcctttatc aggggcgatc gcgaagatgt tgccataaaa tatgtaatta atgaattgaa
    14281 tttagattgt atgtggtcac cagctgctac caccgccgcc gccaccggag cttccaccac
    14341 caaagccgcc accaccgcca ccgccaccga atccgccacc ggagccgctg ctgctggagt
    14401 gtgtgagttt agcaatggta tatcgctcct tgcgttcgtg cccacatgcc ttgcaattgt
    14461 aggttttttc tccttgaccc gtactgtcgt atgtaggtga aacgatagtt ttatttttag
    14521 tcatcagata agccttactc ttacaattcg ggcattcttt atacttgctg taataatttt
    14581 caaatcggaa aatatgaatt tcatttgagt ttggttttcg ccaaacatcg taatctactg
    14641 aacgaatatt ttcttctgca atttgatatg ttttcaagaa ttcatcgtca gctttttcat
    14701 ctaatttcac catcaaggtg ccatcatcag ctagcggcgg aatgtttcgc attgccttca
    14761 tgcgactttt attgataagg gaataaaaaa taaatggaat tggaaaaaat atatccgcca
    14821 ttatccaacc attgctatgt gagcgtgaga ggagattata ggtatcctgt ggtaatttat
    14881 tttcagtatc tctatggata aaatgattta atcgcaagcg tttttccatc aacaaaatca
    14941 ttaataatcc atatgctgcg agagcaaatt caaacacacg taagtttccc gccaattctt
    15001 gccaggcata gaatccaaga ggcaaagcca tattcagcaa accaaatttc gcagtggcat
    15061 atttgctgtt gtattgactt tttacataag ccggagctga tttttcgtgc ttcataccac
    15121 cgcgataacc aatgatggca aacaaaaaat aaactgctgc aaatccaata agcaatccat
    15181 tactagttgt gcgacgccac cacgggaagt tggcatcatt ttcaagtgaa ccggcataaa
    15241 tttcatcatg ataagccgga ttagtgagta tctgataaat ctgcatcaca cccgaaagca
    15301 ctgcattatc catatcaccc tcctttgcat gaggcaccat atatgtttcc tgaatatgct
    15361 tacaaaggat atcaggcaat accccttcga gtccatagcc cgtttcgaac tccatgcgtc
    15421 gttgatccat taccagcaat atcagtaagc cattgtcgtt ttcggactga cctatacccc
    15481 actcgcggaa taattcggtc ttaaaatcac cgggcacttc gctgccaatg gaatttacaa
    15541 tgacaacggc cacctgagca gtagtggaat cttcgagtct acgcaaaaca gtattgatct
    15601 cctcttctac agttgaactc agcacaccat caggattact tacgtatgat gttggcggga
    15661 atggcttggg gatcgactca agggtatagg cagtttgcgc gccggctaaa gcaagcgaca
    15721 aaacaaaaga aaccagtatc agggcttttt tcatctgccc taaatatcgg aaatttttat
    15781 tcctgcggag gggtcctatc cacttttatg cgggcatcct gattggcaag caaccacagg
    15841 gtatggaaaa ccagtcgcgc cctttctgtc atcaaaggga aatcaatctt ttcaatgtcg
    15901 tcggtaggtt gatgataatc ggcgtgaacg ccactgaaat agaaaatgca agggatatta
    15961 ttttttgcga aattataatg atcgctacgg aagtaatatc tgtttggatc ggtagtggaa
    16021 ttgtatttgt aatcgagttt gatattgcta taattctttg ctgctgcttc gttgatatta
    16081 tgcagctcag aactaagaaa atcggaacct ataatatata tataattcgg gtcggcaagg
    16141 tgtgcatcat caacgcgacc gatcatatca atattcaggt cggctacggt atattcaaat
    16201 tttaccacag gattttcggc ataggctttt gaccccaaga gtcccttctc ctcccctgag
    16261 aaagcaacaa acattaccga acgacgtgga ccctcgcctg cttttttagc ttcagcaagc
    16321 gcttctgcaa tttcgagcaa tgctacagtt cctgagccat cgtcatctgc gccattaaat
    16381 acaacagagt cgcgcatgcc aaggtgatcg taatgtgccg aaaccactat cacctgatct
    16441 tttaaatcgg tgccctgaac aaatgcaatt acattatcag catagatttt attctcgagc
    16501 ttattaatat taaaaaccac tgttttatgg atggcaaaat ttctcggcaa tccggtatca
    16561 tcaattgatt gcagcgattt cttcatgagt ttagttcgct tgccgagaag gctttcagcc
    16621 atagcgggtg tgatgaaaaa attattgttg tattccggct gtttatattc actttcaagt
    16681 ttcatgagcg tgccttcgag aaaacgtcgc catgaagggt tatttaaata ttcaggtact
    16741 ttcggttcta tcaccagtaa gcatttcact ccgttacgcg tggcggcctc agcttttttt
    16801 cgccaatctt tagaccaggc agtaacgcta tctttattat ttacgcgtga aattccattt
    16861 aattttggtt cgccatttgc aacgatgaca attttatttt ttacgtctac cccgcgataa
    16921 tcgctgtaaa gtgaatcatc aatgccataa cctaaaaaaa tgatttgatt atcagaaatt
    16981 gccattgaag tatttgccga tgcataacca ataaaatcct gcatcatgtt aaatactgct
    17041 ttttctgtgc tgatggtaga agcattccaa ccgaattcta ttaatggata ttcctgataa
    17101 taaccaccga tagggcgcag tggcggtatg ccgatttgct gaaattgttt cgacagataa
    17161 tctgctgttc ttgcgagtcc gcgcgtgcct gtttcgcgac cttcataata atcagaggca
    17221 attacggaaa ggtgctgctc gagttcttcg gcagtaatgg tgtttgcata ttttacaccg
    17281 agatcttgcg attgagcaaa agttgaagta gctgcgataa tacaaattgc cggcagcagt
    17341 aaatgcaatt tcatcattag aaatcagtca ttttggtaac gcggttggca tgtcggccac
    17401 cttcaaattg tgtgtgcaga aaaacatcaa caaaggcgat ggcatcttcc agagttacaa
    17461 atcgggcagg aatacatatc acatttgcat tattatgttg acgggcgagt gccgcaatat
    17521 ctgattgcca aactaatgct gcacgaatgc caaggtgttt attagcagtc atacaaaccc
    17581 catttccact gccacaaatc aacactccaa aagaagcaat acctgtttca acttcagaag
    17641 ctacaggatg cacatgatcc ggataatcga ctgaatcggc agaatgcgta ccgaagtcat
    17701 gtacttcata acttctgctt tcgagatatt ttatgatacc ctctttataa ggaaatccgg
    17761 cgtgatcgca tccgatggct atgatgtttg tagacattct acaaagatag aacctttacc
    17821 caatcagtcg gaaggtgtaa taatgttaaa aaggactaaa atggcgtcaa tttcctgcca
    17881 attcgcgttt gcggcggcgt ttttcaacca cattcactac aacgatactc acttcataga
    17941 gaccccacaa tggtagagcc accagaaata agctaaaagc atcgcttgaa ggggtgatca
    18001 ctgcggcaag aataagagaa attacggcag catattttct gttctttgca agaaattctc
    18061 ttccgagcaa tcccagcttc gccaaaaaat aagcaaccat tggaatttcg aatattacac
    18121 ctgtccacag taccataccg gtaagaatac caagataaga atcgaatgta aattgatttt
    18181 gtatttgatc gctcaatgaa aatgttgcga ggaaattaat agaaaacgga gccatcagaa
    18241 aataaccgaa gagagcaccc acataaaaca gcagtgttgc aataattatt attcctccaa
    18301 ggttggaaac ttctttatcg tgcaaggcag ggcgaacgaa acgccagatc tcccataaaa
    18361 tgtatggcac ggcaaatatg attcctgcca taaatgcaat ttttatataa aacaggaatc
    18421 ccgaggtcat gttgagataa aacaaatcat aatcgagatt atctacgcaa ataccgtcga
    18481 tattccatct ttcaccgata ttacaaaata tgcggtaagt gataaaatca gggctcttag
    18541 gagcgagaat gattttatcg aaaataaatt gcgggtagat cagaaatgca attgaaaaca
    18601 aaaagatcac cacagctatc cggacgatat gccaacgcag tgcttcgaga tgctccatga
    18661 atgacatctc atccggattc ttctttatcg cagccatact aaataaaaac ccgaattatt
    18721 tactgttaat aagatcatag agggcatcca ggttcgggct gaggatgatc tctgtgcggc
    18781 gattgcgcgc cttaccttcg gttgtggtgt tatcggcaat agggaaaaat tcaccgcgac
    18841 cttcagcgat cacgcgttta gcgtcgacac cattttcctg agtcagaatt tttacgatac
    18901 ttgttgcacg caatacacta agatcccagt tgtcctttgg aaaattattg ctttttagcg
    18961 gtacattatc ggtatgacct tcaatctcaa ctctgatatc gggatttgcc ttcaacacat
    19021 cagcaagttt tttcaaagca tctttccctt tgggatccac aacggtactt cctgatgcga
    19081 ataataattt ctcgctgagt gatacataca cttttccatt tttgcgctca acggtaagat
    19141 cacttgcact gaatcctgaa agggccttat cgattgtatt tttaagatca gtaactgttt
    19201 gttcctgttt atttagaata gattcaagtt cctctacacg cttgctgcgc gcttcaagat
    19261 ctgcagtcaa tgcatcaata cttttttgct gatctgaaag cgtcatcgcc ttagcattca
    19321 attctttttc tttagctatc agttcatcag actttttctg aaggtcttca cgcaattgct
    19381 gattttcgtt tgcagataat gccaacaatt ggttaatgcg attgttgagc acatcttcaa
    19441 tttcacgcaa ttgctcattc gccttcacca tagcatcata gcgattgtgt atatctatgg
    19501 aatctttgcg catcagatta atagatgcat acacctcatc aagttgctgg gaaagttcat
    19561 cgacttcttt tgttttcata cgcaattgac tgtgcaacac tgctgtgtcc tgcatgcagg
    19621 catcgcgagc ggtaatggca tcctgatatt gacgctgcgg cacacaagcc gaaaaagcca
    19681 atgctgataa tgctataatg ctgagttttt tcatattctt catgtttaac tgggcgaaat
    19741 aaacggtttt ttattattgt atcgtattcg ttatcaacgg cgtttgagca taccgagcaa
    19801 tccacgctcc aatacgttaa tcaccgtacg ggtgatctga tgctgtgtgg tcttgtcgat
    19861 caatggtttt tctttggtct ttttcacttt gcgctcgcgt tcttcgagtt tctgtttggc
    19921 ttcttcctcc ttcaattgct tttcccgctc agccgcttgt tcacttgcgg ccagtttttt
    19981 atttaatatt tcataggcac tctctctgtc gacagattca ttatattttt ttgttaacga
    20041 tgaaatatta ttcaactgct gcatttcttc tgctgaaaga acatccatac gcgactgagg
    20101 tgccaccagc aaggtatgtg ctaaaggagc gggtgcacct ttttcgttga gtgccgtaat
    20161 gatggcttca ccgataccga gatttgtaag gagatcttct gtttgataaa aatcggtaat
    20221 aggataattt tctgctgtca gtttgattgc ttttctatca tttgccgtaa atgcgcgcaa
    20281 ggcatgttgc accttcaaac ccagttgcga caatacttct gcggggatat ccgttggcaa
    20341 ttgtgtgcag aaaaatatac cgacaccttt agagcgtatc aattttacga tagtctggat
    20401 ttgctccagc aaacttttac tggcctcttt aaagataaga tgcgcctcat ctatgatgat
    20461 taccagctta gggtcttctt tgtcgccaat ttcaggaaag gtattatata cctcagccag
    20521 caaacacaac ataaatgttg agaataactt cggcttactt tgaatatctg tgagtcgcaa
    20581 gatgtttacg tatgcctgac ctctgtcatt catgcgacag agatcttcca catcaaaact
    20641 tttttctccg aagaaaagat cagcaccttg ttcttcgatc tctatcactt ttcgcaaaat
    20701 ggtgccggcg cttgatgtgc tgatggttcc gtaatctgcg gagatatctt ttttcccttc
    20761 ggaagtaagg taattgagtg ttttacggaa atctttcaga tccaggagtg gaattttgtt
    20821 atcatcgcaa tatttgaata ccagcgacac aacgccttcc tgaatatcat tgagttcaag
    20881 gatcttagaa aacaatacag gaccaaattc cgaaactgta gcacgaagtc gaacaccatt
    20941 ttcattgctg atgctcatca attcaacagg atatcctttt gcctgccagc tttttccaat
    21001 ttgctgcatg cgttcatcaa ttttcggatt tcctgttccg ggcatagcaa ttccactcac
    21061 atcgcctttg atatccatga tgagcacacc aacaccatta tcacttaatt gctctactat
    21121 gccttgcagt gtttttgttt ttccggttcc ggtagcgcct gccaccagtc catggcgatt
    21181 catcatcctc aatggcactt ttacatgggt ttgaggcata ggctggcggt cgagaatagc
    21241 agttcctaag acaattgaag tcccttggaa gttataccct tcttgaatta cctgcttgaa
    21301 ctgttcgttg cggtccacaa aaaatgattt aatttgtggc aaagatacac gttatctatc
    21361 gtggattact gatatggcca acaaagcaaa aacagccttt ttttgtcgaa actgcgggtt
    21421 tgaatcttcc aaatggctgg gaaaatgccc ttcctgcaac gaatggaaca cttttaccga
    21481 ggaggtgata gaacgcccca aggatgcggc ttcgtggaag gatagcaagc ctgaaaaacg
    21541 catttccgga ccggtgcaat tgtcggagat caccgcaagc gataccggaa ggctggtaac
    21601 cggcgatctg gagctcaaca gaacattggg aggcggtata gtggcaggaa gcctggtact
    21661 gattggtggt gaacccggta ttggaaaatc aacattactt cttcaattgg cactcgaact
    21721 gaagcaaatc aaggttttgt atgtttcagg agaggaaagc gagcaacaaa taaaaatgcg
    21781 tgccgacagg ttgaagggca ataatcaggg attatatatt tctacggaag ttgccatcag
    21841 caaattgttt atgcaaataa aacaattgca acctgaaatg gtggtgatcg attctattca
    21901 aaccctggtg agtgattttg ttgatgccac agcggggagc atcgcacaga taaaagaatg
    21961 tgccggtcaa ttgcaacgat tcgctaaaga aactaatgtg ccggtattta ttatcggtca
    22021 tattaataaa gaaggttata tcgccggtcc gaaaattctg gaacatatgg tggatacggt
    22081 attgcaattt gagggtgaca ggaattacac tcatcgcatt ttgcgcacca taaaaaatcg
    22141 ttttggttct accagtgaga tcggcattta taaaatggaa ggtgatggat tacaacaagt
    22201 aaataatccc agtgaattat taatcactga aaaagaagaa cctttgagcg gtgttgccgt
    22261 tgctgctaca atggagggta tgcgccctat gcttattgaa gtacaagcat tagtgagtcc
    22321 ggcagtatat ggtaatccgc aacgcacagc tacaggattt gatttacgca ggttgaatat
    22381 gttgcttgcg gtgttagaaa aacgcggtgg ctttgcctat gggaataaag atgtgttttt
    22441 gaatattgcc ggtggcttgc gtgttgaaga tcccgctatc gatttgagta tcgcatgtgc
    22501 attgttgagt tcactgactg atatggcatt accactgaat atgtgttttg cagcggagat
    22561 aggcttaagc ggcgaattgc gcggagtagc aagaattgag caacgcataa ctgaagcaga
    22621 aaaattaggt tttgatcgca tttacattgc caagcacaac atgaaatcgc tcaagcaaaa
    22681 taaatacaat attgaattga gggcagcagg aaaattggaa gaagtatacg gtcagttatt
    22741 tgtgtgatga attttttcca cattactctt atttgaaccg gcggatattt catggccata
    22801 aattcgcata agcgtttata agcgcgttaa tcgacatttt gttgatttta ctcctacact
    22861 tccatttcga tagtgactaa attatcgaaa tgtttccctt attactgtta aatgaattgt
    22921 cggatatgtt ttatccgcgc ttatgtacgg catgcaaaga gccattgctg gcacatgaac
    22981 atattatatg tttgcgctgt cggattgaat taccgcgcac cgatcatggc ttatttcatg
    23041 acaatccggt tgctgtaaaa ttctggggaa aggtgccgat agaggaagca gcggcgatgt
    23101 tacattttca caaaggatca cgggtgcagt cgctcatgca tgcactgaaa tataaaaatc
    23161 gaacagatgt agggattacg ctgggtaata tgttaggata ttattgcaaa caatatcatt
    23221 tgtttaaaca gattacagcg ataacagcag tgcctttgca taaagaaaga ttggcagttc
    23281 gcggatacaa tcaaagtgct gtaattggcg aaggtctggc tgaggtattg caagttccat
    23341 ttaaaggtga atggctagag cgcatcacac ctactgctac gcaaactaaa aaatctcgat
    23401 tggcgcgttg ggataatgtg aaagatgtat ttcgtgtaac tgatcccgat ggaattgcgt
    23461 atcagcatat tttgctcatt gatgatgtaa ttacaaccgg gtcaactttg gaggcatgcg
    23521 ccttaaaact catacaatct gtaggggtga aggtgagtat agccgcgatt gcctttgctg
    23581 atcgctgagg cctggacttg acatacatgg gcaaaaagca gcttgtttgg gaaacacgcc
    23641 tgattccctg aacaatcgaa aacaggcctt aaactcaata ttgacaggcc ttttgtggga
    23701 ttttgactct aaaaagcaaa aaaatatgtc aaaacgcttg cgcaactgtt tggttgctgt
    23761 tatatttgca accaattagt tgcgcttaga gttttagtat tcgctattac aaattcaaag
    23821 cccggatatt tgacacgcac aataaaattt taacaaccca cgcatgagtg aaaaagacaa
    23881 ttggatcgcc atctttataa aaatatggga atcgagattc gatcagttag acgtgatatt
    23941 gcataaactc aaaaataaaa gacaatgaac aaaactattc tattcgactt caatgtcgac
    24001 aaagaggcac gcacgatacg tgtcgaccga tcatttgatg cgccgcgaga tcttgtatgg
    24061 gcggcatgga cagaatcaga tattttagat caatggtggg caccgaagcc atatcgaaat
    24121 gtaacactga gcatgaattt tgttgaaggc ggtcgctggc attattacat gatctcgcca
    24181 aaggatgaga aacattattg cctgtttgat taccatcgca ttattccgga aacgatgtac
    24241 tcaggcatgg atgctttttg cgatattgac ggaaatattt cggagagtat gccgcgcatg
    24301 caatgggaaa atagtttcga aaaacaggga gataccacag tggtgcatgt gctcatcaac
    24361 tttaataaaa ttgaggacct tgaagcggtg atcagtatgg gctttaagga aggatttagc
    24421 atgggaatgg aaaatctgga cgcctatatc gcttctcaat tttatcttcg gaaatcaaaa
    24481 aaaacaagta atgccgcacg tgtatgcact tatttaaatt ttcccggcaa tactgaagat
    24541 gctatgctct tttataaagg gatttttaaa acagaatttg tacaaggcat acaacgattt
    24601 ggcgatgtcc cttccgacgc gggtgctcca cctgttccgg atgcattgaa acaaatggta
    24661 ttgcatgtag aacttccact tataggtggt catatcctta tggcaaccga tgcacctcaa
    24721 gaaatgggat ttgaagtgaa ggtcggaaac aacatgcaca tcagccttga acccgattcc
    24781 aaagatgaaa caacgcgcat tttcaatgca cttgcagaag gcggaaatat aacaatggaa
    24841 ttacaagata tgttctgggg tgcctattat ggtagttgca cagataaata tggcattaac
    24901 tggatgttga attttcagca acattgatat agtcaaacca caatctagaa ccggagtgag
    24961 cctttcgctc cggttttttt attaaaaaaa ctcggtctta taaggatagc gtatctgata
    25021 ttgttcttta accatatcag tcagcataga ccttagcaat tcgatatttg ttctgttgat
    25081 ggctgaaata aaaacacatt gagcatttaa acgctgcatc cacgtgtttt ccagttcact
    25141 taggatatcg cgctttacat caggcgtgag atacttatca aaataccgct cttcgtaagc
    25201 atccatttta ttaaaaatga gaatggtagg tttatcaata gcctctaatt ccttcagcgt
    25261 ttcattcacc acatgaatat gttcttcaaa tgccggatgc gacacatcaa ctacatgcag
    25321 aagaatatca ctttcacgca cttcatccaa tgtggattta aagctttcga tcaggtgatg
    25381 gggcaatttt ctaataaatc caacggtatc actcaacaaa aatggtgtac gatccaacac
    25441 tactttgcgc acagtggtat caagtgttgc aaataattta ttttcggcaa atacttcact
    25501 tttactaagt aaattcatca aagtgctttt ccctacgttt gtatatccaa ccaatgcaac
    25561 acgaattaat tcgccgcggt ttttgcgttg tgtttcattt tgcaagccaa ttttttcgag
    25621 ttgttgtttc aacaatgcaa tgcgttgctg agctaccctg cggtcggttt caatttcttt
    25681 ttcaccggct ccgcgcatac caataccacc tttgatcctg tcgaggtgac cccacaagcc
    25741 ttttaatcgc ggaagcatat actgggtttg tgctaattca acctgcgttt tcgcctgcac
    25801 ggtttgagct ctggtggcga aaatatcaag aatgagcata gacctgtcga gtacttttac
    25861 ttttaaagtt tcctcaatat tcctcaattg cgaaggcgaa atttcatcat caaaaatgac
    25921 gatatctatc tcatttgcca ctacataatc cattatttca aacaacttgc ctttgcccac
    25981 atacgacttt gtatcaggat gcgggagctt ctgataaaat ttcgctttgg tgactgctcc
    26041 ggcagtttct gctaaaaatt ccagctcatc cagaaattca tttaattgct caagcgtctg
    26101 gtctttggta accagtccga cgaggataca tgtttccgtt tcgttaggtt tgatggtagg
    26161 tttatgcgcc atattgattt attgccgttg gcgatggtag taaaaccacc tatatttgcc
    26221 tgaagacggc gtttctacca caaaggtaat tctttgcaac cgaacgccgc taaactacaa
    26281 atcgctgcat gcagatcaat ttccttcgac ataatgagat cgaccgaaaa aattgggatc
    26341 gctgcatcga caatagcctc aatgctttgc cttacgctta tagttggtat ctggacatag
    26401 tgagcaatgg acaatgggat gcaataataa ttgatgatta tgccgctgta tttcctatac
    26461 cctttaaaaa cagattgata tacaagcagg tttaccaacc attttttacg cagcaacttg
    26521 gtttatttta tacagataaa ttataccata caaaattgcc ggatgtattg aaagctctac
    26581 ctgcatccta tcgaaaaata cacttgcatc taaatataga aaatgatttg ggtgaagtat
    26641 tttctgctgt tgaaaaacgt gttacacatc acatagactt aaacaaatcc tatcaatcca
    26701 tagcttctga ttataacgag caaattcgac gcaatctaaa gaaatcattg acacatcatt
    26761 tacaagttga aacttcttca gatgtcaata ttcttatttc tttgcgtgaa atgtttcttc
    26821 aagaagcgtt aaagggaaaa caaagtgctg cagatacaga ccgattaaaa aaacttatcc
    26881 aagcaatact cgacaataaa aagggtgaaa taaaattcgt tcgcggcgct gaaaatgaca
    26941 tccaggctgc tgtctgttta cttcaaagta acggctacat catttatctg tcgagtgttt
    27001 caagcgatgt cggcaaggcc aatcacgcca tgacattttt gattgacgat atgctcaaat
    27061 ccaatgccaa tacacagcag accttcgatt tcgagggctc tatgataccg ggaattgccc
    27121 gatatttcaa aggattcgga ggcaaagaaa aatggtttag cgttattcaa aaataacgtc
    27181 agacagcatc aatatgcgta caatggaaac tgcgaagcga aagcatttac ttcgtttcta
    27241 acggcagttt tcacagcatc atcatcaggt gccatgatta atctgtcgat ccactcaaca
    27301 atacgcacca tatcattttc tttcatgcca cgagtagtaa cagcgggagt tccgatacgt
    27361 atacctgaag taacaaatgg cgatttatcg tcaaacggca ccatgttttt attcacggtg
    27421 atatgagcat ctaccaataa tccttctgct ttttttccgg agatattttt attgcggagg
    27481 tcaataagca tcagatgatt atctgtacca ccgctgataa ttttatatcc tttgctaaca
    27541 aatgcattcg ccatagcttg tgcattagcg atcacttgtt tgcaataggc aacatatcct
    27601 tcatcaaggt cttcaccaaa tgccactgct tttgcagcaa taacatgttc cagcggtccg
    27661 ccttgtgtgc cggggaaaac agcgctatca atcacggcac tcatcatttt cacttctcct
    27721 ttgggagtag tttgtcccca agggttagga aaatcctttc ctatcatgat catacctcca
    27781 cgtgggccac gcaatgtttt atgcgttgta gttgtaacga tatgacaatg aggcataggg
    27841 tcgttgagca attttgctgc aattaatcct gcagggtgag aaatatctgc caataaaatt
    27901 gcacccactt tatcggcaat agcgcggaag cgggcataat cccaatcacg ggcataggca
    27961 ctagcgccgc agatgatcag ttttggtttt tcggcgattg ctgttgcttc cactttatcc
    28021 atatcgatgc gacccgtttc ttcctctacc gaataaaata cagggcgata aagttttcct
    28081 gaaaagttca ccggactgcc gtgcgtaagg tgaccaccat gtgagaggtt gaatcccaat
    28141 aatgtatctc cgggattaag gcaagcgagc ataactgctg catttgcttg cgcaccgcta
    28201 tggggttgaa cattagcata ttcagctccg aataattttt tggcgcgatc aatagcaata
    28261 ttttcagtta catctacata ttcgcatccg ccataataac gttttccggg atatccttct
    28321 gcatatttat ttgtcataca gctacccatg gcttcaagca cctgatcgct cacatagttc
    28381 tcgctggcaa tcaattcaat gccttcagtc tgacggtgga gttctttctt aatgatatca
    28441 aatacttcag tatcgcgacg cataattatt ttctttgaaa caaaattacg tattcagcgg
    28501 caatttggta tatgctgata cccataggga tgtttaatgt atgcatatag atacatttag
    28561 tcacaaaaag gggtgcaatt aaaataaata cttgttactt taagtggttg cagctatgaa
    28621 acaattgttt tgaaggtggg tatttttgat gattaaagat tcggcaacca ccttaaaaac
    28681 atatctacac aaatattatc ccaaataaat caatttcttg attcttttaa ataaaaaagc
    28741 ccgacttata gagccgggct tcagatgtaa tttggaaatt atttttttta cttcgccagc
    28801 attacgcgtt cgttgatgct ttggttaccg gcagtaaggc ggatgaaata catgccattt
    28861 gcgaggtgat atttattcag gtcaactgaa acaatatgtt caccttgagc gcctgagatc
    28921 agttggtcag cacccagatc ggcgatgagc tgaccggtaa gtgaatatat agtcaactgc
    28981 atgttatcct gtgctgcact caatccgtaa tgcacttgaa caggaccggc tgtaacagac
    29041 ggcacagctg tcattgacac agaaacccca ttttcttcga cgcttgtttt caacctgaag
    29101 tgcgccacgg cattttcaat atcagataag gcgaagaaaa tatccggagc aaatggatca
    29161 gggttggcag tatgattttc gagtgaccaa taatctaaaa cgtaagaacc tgtaggagta
    29221 gctgaaaggt ctatgaaggt gccactttca tatgcgcctg cataagggta agctgcagga
    29281 gaaaatcctt caactgttgc aacacctgct cccggaggat cgatgtctaa ggtgaaatca
    29341 tacatcggca attcactacc tgcaaaccat gcaattatgg tatcagtagt tgttaaagaa
    29401 acagttactg aatcatctgt tgtagatgga gaaggcacgt ggtttataaa ctcccaatga
    29461 tcgaaagtaa atgcaggatc tgcgatagcc tggaaaccta aagtggtgcc gctgaaataa
    29521 gtagctgaat aaggataagt atccggaata atggtactta cgcgcacatc attcggactg
    29581 ccggcaggtt caattttcac aacaatacca taggccgtta cgtcgaaaca atcttcaaca
    29641 cctgatggca ggtatgcaca acgttctgca ataaaatcgt gcaattcatc gtagttattg
    29701 ctccatcctg tatatgttcc gccccattta tcggtgtgtc gagtcatttc aggatcgagc
    29761 actgcgcgca tgctatcgat acgtggcaag agcacctcac aactaaaggt agtattgatc
    29821 aaatcagcaa aacgattaat ataatctgaa tggaaatccg ggttatccaa taatgagttt
    29881 aacatatcaa tatgtccatt ggggtcaaca ccaccaagtg taagtggatc gcaagggctg
    29941 gcagttggtg aatcatcagg aacgccggta taattgatat agtgtccgaa tgtagcatct
    30001 tcatcccaca atgcatagcg ccatgtgaga ttcgaacctg ttgcatcata gccgcgccac
    30061 caggccgtat tccagttcaa ccagtcggta caaaccgaga atgtatttgt taatacataa
    30121 tcgatgagac tcatggtgtt aaaattatct tttacataat cgtaattagc gggaatggtc
    30181 atatcgttgg tagtaacgaa tgtaacgaac gggctccacg gagccatggt accgtattct
    30241 gcccatgttc caccccaaca tttgatataa tccatttcat attctccctg accatagtag
    30301 tagtcggtat aatcggcgtc atcagctttc tcgcgggtat cgtatacgcc ccaataatcg
    30361 ccattgagga acatgatatt aaagaaagtt gaacgttcat ctaattcgag gccatccaaa
    30421 cctgaaatat gatgaacaaa ggcatcgcgc acatgggcgc caccgcttga agatggataa
    30481 ttgtcatttg cagcggcttt aacaatcaat cgctgatacc cttcgcgatc agtaacgtcg
    30541 ttaaaaaagg tttgatcgat atcattatca taaccgaatt gatcgcgaac aataaaatcg
    30601 aaaccacgtt gatcgtaagc ccatgaatcg tttccatgtt cgttaaaatc accaacagct
    30661 tcatctatga gattaaaatc tttatcgaat aattcaaaag atcccacagg ttcaattccc
    30721 catcctgttg cgttcaataa atcatcaacc tggtcaccgg caactgaaat aacgggcata
    30781 gtagttgtct cgtcaatgaa ataggtattt gtagaacaga aacttgccag tgtatcggct
    30841 gaagccgcga atgccacagt gcgcagaact gttgtttctg tgatagtaat cggacctgta
    30901 taagcagttg atgccgcggt aggaaggttg ccattagttg tatagcgaat cgtcattccc
    30961 ggttcgggag cagtaatagt aacagttaca gaacccgcaa aattgccgga attcggttcc
    31021 atgtcaggct tatgtgcata tgctgttttg ggcgttgttg agttagatgc atttggcgtt
    31081 gggtctgtaa atattttcca atctgctccg ccatttggat agcgtcccca actgtgattt
    31141 gtttgattag gattatttaa ttcgcgtgta tccaatactg ttcctgcagc gtcagcaaat
    31201 accacatcct cagctccggt agtttgtgtg agcttaaaat tagtatggta atttgtgcct
    31261 actgcggtat tcctgttcga acaccaaaag cgcaaatagc cacctgcaga aatattcact
    31321 ccgggcggaa tggtgtattt tgtcggtgaa cctattctgt cgctcaggtg atatcctgaa
    31381 agatccactg ctgttgcacc ggcattgtat aattcgatcc agtcttcata ttcaccgtaa
    31441 ttatcggcga tatcgctgta gttggctgca gaatattcgt tgatcaccac ctgacctgaa
    31501 agaccaagta ttgtcaatcc aaatacacta cctgagagga gtaatttttt gagcatacga
    31561 tgtatgtatg aatgttaaag gcaaactaat tcaacctcta aaatacgtaa cattttgata
    31621 attaagattt atcaatcgag gattttgtaa ggcatttgtc acatttggct aaaaaaatgt
    31681 aagaatatgg ctccagaaag cctggccaag ccctttctta gcatggttta aattctacct
    31741 ttgtgccatg aaagtgatca tcccggtggc aggtgtaggc acccgattgc gcccgcatac
    31801 ctattcgcag cccaaacctc tgattccggt ggcaggcaag cccatattgg gtttcatcat
    31861 cgacaggatg gttaaggcag gattcacaga attcattttt gtgataggat atttgggaga
    31921 aaaaattgag tcctatgtgc ttgaacatta ccctgaatta aaatgtcgat ttgtcataca
    31981 aaacaagcgt gaaggtcttg gacatgctat tttccttacc aggccattaa tttctgaaaa
    32041 tgaagagttg tacattgtgc tgggagatac catttttgaa gctgatattg aagctgtgat
    32101 gaccatgccg gagtcggctc tgggactgaa acgggttgat gacccgcgcg attttggggt
    32161 tgccgaagtt gaagacgata tgcgcattac aagggtgatt gagaagccct ccataccgaa
    32221 atcgaatttg gcactggttg gactttacaa gatcaaagaa agtgctcagc tgttcaatgc
    32281 cctggagcag atcatggcac acggttataa aacgcaggga gaatatcacc tgactgatgc
    32341 attgatgatg atgataaagg atggtatcac attccgcggt tttaaaatta ccaattggta
    32401 tgactgcgga aaaaaggaaa tattgcttga aacaaatgcc atactgctga aaaaactgca
    32461 tcctcaaggg cagcatcaac atgcctatcc gaataccatt ctcatagagc cggtaagtat
    32521 tgctccgggt tgtgatatac gtgattctat tattggtccg aatgttaccc tgggagaaaa
    32581 tacatctgta cactacagca tactccgaca ttccattatc ggcaacgatg ccaccattga
    32641 tgatgtggtg ttgctcgata gcgttatcgg ctctgatacc gtaatcaaag gcatgagcca
    32701 gagcctcaat attggcgaca atacggagat cgatctttcg aagagtgggt gagtggtgag
    32761 tggtgagtgg gtgagtcgtg aatggtgagt ggtgagtcaa gggtaaatcc atggaatatt
    32821 aca
//
LOCUS       DAOQOU010000014        32804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_599_length_32804_cov_6.414242, whole genome shotgun sequence.
ACCESSION   DAOQOU010000014 DAOQOU010000000
VERSION     DAOQOU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 32804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32804
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_599_length_32804_cov_6.414242"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /gene="rplB"
                     /locus_tag="PLM90_02255"
     CDS             <1..592
                     /gene="rplB"
                     /locus_tag="PLM90_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HMZ88218.1"
                     /translation="KTVEYDPNRTAFIALLVYADGEKRYILAPNGIQVGQTVQSGLNA
                     APELGNALMLRNIPLGTIIHNIELYPGRGGMIARSAGSWAQLSAKEGKYVIIRMPSGE
                     TRMILDTCFATVGTVSNPDHNLQQLGKAGRKRWLGRRPRNRGVAMNPVDHPMGGGEGR
                     SSGGHPRSRNGQIAKGLKTRQPKKTTSKFIISRRKK"
     gene            604..867
                     /gene="rpsS"
                     /locus_tag="PLM90_02260"
     CDS             604..867
                     /gene="rpsS"
                     /locus_tag="PLM90_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HMZ88219.1"
                     /translation="MARSIKKGPYVDHNLAGKVAQMNESGKRTVMKTWSRRSTILPEM
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFSPTRNFKSHAGNR"
     gene            873..1256
                     /gene="rplV"
                     /locus_tag="PLM90_02265"
     CDS             873..1256
                     /gene="rplV"
                     /locus_tag="PLM90_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HMZ88220.1"
                     /translation="MEAVAKLINNPRTNNNSGSPRKARYVVDMIRGLDIDKALSVLKY
                     TNRRNVTAVEQTLRSAIKNWESKYEMDAADSNLYVKEAFVDGGKTMRRFLPAPHGRAY
                     RMRKRTNHLTIVVASRNANNQEKNA"
     gene            1274..1981
                     /gene="rpsC"
                     /locus_tag="PLM90_02270"
     CDS             1274..1981
                     /gene="rpsC"
                     /locus_tag="PLM90_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HMZ88221.1"
                     /translation="MGQKCNPIGNRLGIIRGWDSNWYGGKTMAEKLVEDEKIRQYLNA
                     RISKGGISRIVIERTLNRITVTIHTSRPGIIIGKGGGEVDKLKEELKKITEKEVQINI
                     IEIRRPELDANIVGETIARQLEARINFRRAIKMAVASTMRMGAEGVKIRVSGRLNGAE
                     MARTEEYKQGRVPLHTFRADIDYSWKEAHTVYGKIGVRVWICKGEVLGKRDLSPNVGM
                     SQEKERSKQGRRRPNKR"
     gene            1996..2415
                     /gene="rplP"
                     /locus_tag="PLM90_02275"
     CDS             1996..2415
                     /gene="rplP"
                     /locus_tag="PLM90_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HMZ88222.1"
                     /translation="MLAPKKVKHRKTFKGKMKGDAKKGSQLSTGSFGLKTLESGWITD
                     RQIEAARVALTRHLKREGQVWINIFPDKPITKKPAEVRMGKGKGNPELWVAVVKPGRI
                     MFEAEGVPLDTAREAMRLAAGKLPVKTKFVVRRDYVA"
     gene            2430..2639
                     /gene="rpmC"
                     /locus_tag="PLM90_02280"
     CDS             2430..2639
                     /gene="rpmC"
                     /locus_tag="PLM90_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HMZ88223.1"
                     /translation="MAKLKREDLNQLSNEDLQQRLHDDSQHLTKLQFTHAVNPIDNPS
                     QLRSIRREIARIKTEIRKREIASAK"
     gene            2651..2914
                     /gene="rpsQ"
                     /locus_tag="PLM90_02285"
     CDS             2651..2914
                     /gene="rpsQ"
                     /locus_tag="PLM90_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HMZ88224.1"
                     /translation="MEQSTRKLRRTIIGVVTSDKMDKSITVTVERKVMHPKYGKFLKK
                     SKKYHAHDEANDAKSGDLVKIMETRPLSKLKRWRLVDIVERAK"
     gene            2916..3284
                     /gene="rplN"
                     /locus_tag="PLM90_02290"
     CDS             2916..3284
                     /gene="rplN"
                     /locus_tag="PLM90_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HMZ88225.1"
                     /translation="MIQQETRLKVADNSGAREVLCIRVLGSTRRRYASLGDKIIVTVK
                     DALPGGGIKKGTVSTAVVVRTHKEVRRKDGSYIRFDDNACVLLNASDEPRGTRIFGPV
                     ARELRDKQFMKIVSLAPEVL"
     gene            3287..3634
                     /gene="rplX"
                     /locus_tag="PLM90_02295"
     CDS             3287..3634
                     /gene="rplX"
                     /locus_tag="PLM90_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HMZ88226.1"
                     /translation="MKETNYSNRFQPKYHVKKNDTVVVIAGADKGKKGRVIEVDYAKN
                     RVMVEGVNIVSKHTRPTQQYPNGGIIKKEAPIHISNVMPVDPKSGKGTRVGRKEENGK
                     SVRYSKKSGEIIK"
     gene            3634..4188
                     /gene="rplE"
                     /locus_tag="PLM90_02300"
     CDS             3634..4188
                     /gene="rplE"
                     /locus_tag="PLM90_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HMZ88227.1"
                     /translation="METKSPRLKKQYHETVVKDLQEKFQYKSSMQVPKLLKIAINQGL
                     GAATQDKKVVDVALDELTRITGQHAVSTISKKAISNFKLRENMPIGARVTLRGNNMYE
                     FLDRLISVALPRVRDFKGVNNKSFDGRGNFTLGITEQIIFPEIDIDKVTKITGMDITF
                     VTTATTDAEAYELLKGLGMPFRKA"
     gene            4198..4467
                     /gene="rpsN"
                     /locus_tag="PLM90_02305"
     CDS             4198..4467
                     /gene="rpsN"
                     /locus_tag="PLM90_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HMZ88228.1"
                     /translation="MSKKSVIARNNKREQMIIKFAERRKQLKAEGNWEALDKLPRNAS
                     PIRYKNRCKLTGRPRGYVGDLGISRIKLRDLALYGKIPGMTKASW"
     gene            4493..4894
                     /gene="rpsH"
                     /locus_tag="PLM90_02310"
     CDS             4493..4894
                     /gene="rpsH"
                     /locus_tag="PLM90_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMZ88229.1"
                     /translation="MKVTDPIADFLTRIRNAQKANHRIVEIPSSQMKQRLTEILYENG
                     FIYKYKFEQEGPQGTIKIALKYDPVTKSPAIKGIERVSSPGLRRYTGVDSLPRVMSGL
                     GLAIMSTSKGVMTEKEARRQNVGGEVLCYVY"
     gene            4917..5471
                     /gene="rplF"
                     /locus_tag="PLM90_02315"
     CDS             4917..5471
                     /gene="rplF"
                     /locus_tag="PLM90_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMZ88230.1"
                     /translation="MSRIGRLPIQLPAGVTLSVAGNNMVTIKGKRGELTLQVDPDITV
                     KTEDNAIKLERPSEQKRHRAMHGLYRALIANMVKGVSDGFKKELELVGVGYRAANTGQ
                     ILEISVGYSHPIMMMIPKEVKLSTVSEKGKNPTIILESNDKQLLGAIAAKIRSFRKPE
                     PYKGKGIKYSDEVLRRKAGKTAGK"
     gene            5483..5839
                     /gene="rplR"
                     /locus_tag="PLM90_02320"
     CDS             5483..5839
                     /gene="rplR"
                     /locus_tag="PLM90_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMZ88231.1"
                     /translation="MLSKKSIRRQKIRYRIRKKIAGTAECPRLAVFRSNKEIYVQLID
                     DVAGHTLCAASSNDKQGKAYTGTKVEKAAAVGKQIAAQAQAKGITTVIFDRGGYLYHG
                     RVKSLADGAREGGLQF"
     gene            5855..6376
                     /gene="rpsE"
                     /locus_tag="PLM90_02325"
     CDS             5855..6376
                     /gene="rpsE"
                     /locus_tag="PLM90_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMZ88232.1"
                     /translation="MAVNKDIQRVRASELELKEKIVGISRVAKVTKGGRTFSFSALVV
                     VGDGNGTVGHGLGKAREVTEAIQKGIDDAKKNLIKVPVFNGTIPHEQHGKFGAGKVMI
                     KPAAHGTGVIAGGAMRAVLESAGVTDVLAKSLGSSNPHNVVKATLDALAHLRSPLAVA
                     QHRNVSVKKVFNS"
     gene            6400..6579
                     /gene="rpmD"
                     /locus_tag="PLM90_02330"
     CDS             6400..6579
                     /gene="rpmD"
                     /locus_tag="PLM90_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMZ88233.1"
                     /translation="MAKIKITSVKSTTDRPKNQKATMKALGLRRMHQTVEVEATPQII
                     GMVRVVQHLVKVENV"
     gene            6581..7033
                     /gene="rplO"
                     /locus_tag="PLM90_02335"
     CDS             6581..7033
                     /gene="rplO"
                     /locus_tag="PLM90_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HMZ88234.1"
                     /translation="MDLSNLKPASGSIKKRHRIGRGQGSGWGETSKRGFKGAGSRSGT
                     STKRGFEGGQMPLQRRLPKRGFNNKNFETKYVTLNLDQIQEMVDKYEITKLDQDMLVA
                     NRIIKGDDKLKVLGRGELKAKVEIWAHACSETAQKAIEAAGGTITMIG"
     gene            7037..8392
                     /gene="secY"
                     /locus_tag="PLM90_02340"
     CDS             7037..8392
                     /gene="secY"
                     /locus_tag="PLM90_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMZ88235.1"
                     /translation="MKQFIQTLKNIWSIEDLRKRIIYSLGLILLYRFGSYVMLPGIDA
                     GIYNQAANVDSQSGLLGLINLFAGGGFSRASIFALGIMPYISASIAIQLLGIAVPYFA
                     RLQKEGESGRRKMNQYTRYLTVIVTALQASGYYAYLNTMSKNADFIVAPQGLFWFITV
                     VILTAGTLFTMWMGERITDKGIGNGISMIIMVGIIAGLPAALVNEITHRGAASGGGFI
                     PFLLEIAFLIVVIMGCILLVQGTRRIPVQYAKRVVGSRQYGGVRQYIPLKINAAGVMP
                     IIFAQALMFIPGLFAQINEDWQSSPVLVMLNDFQSLGYNLIYGFLIVLFTYFYTAIII
                     NPSQIADDLKKNGGFIPGIKPGKKTSEFIDNVLSRITLPGSIFLALVAISPVLTRAFH
                     VDPSFARFFGGTSLLIMVGVILDTLQQIESHLLMRHYDGLMKSGRIKGRTNAPVSIQQ
                     G"
     gene            8389..9201
                     /gene="map"
                     /locus_tag="PLM90_02345"
     CDS             8389..9201
                     /gene="map"
                     /locus_tag="PLM90_02345"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMZ88236.1"
                     /translation="MIYYKTTDEITLIRKSSLLVSETLAMIAGIIKPGVTTLWLDQQA
                     EAFIRDNGGKPSFKGYKGYQHTLCVSVNDQVVHGIPSAREIKNGDVVSVDCGAFLNGF
                     HGDSAFTFLVGEVSREAVNLARVTRACVYKGIEFAVAGNRVGDVSHAIQLYAEHVNGY
                     GVVRELVGHGIGKNLHEEPEVPNFGAKGKGPKMQEGMVIAIEPMINLGSRNVVQENDG
                     WTIRTRDGKVSCHYEHTVAVMKGKADVLSSFEIIDEAMAKNSNLYTEKEIVG"
     gene            9201..9419
                     /gene="infA"
                     /locus_tag="PLM90_02350"
     CDS             9201..9419
                     /gene="infA"
                     /locus_tag="PLM90_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMZ88237.1"
                     /translation="MSKQDLIKQDGTVTEALSNANFRVKLENEHEILATISGKMRMNY
                     IRILPGDRVTVEMSPYDLTRGRIIYRYK"
     gene            9429..9545
                     /gene="ykgO"
                     /locus_tag="PLM90_02355"
     CDS             9429..9545
                     /gene="ykgO"
                     /locus_tag="PLM90_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HMZ88238.1"
                     /translation="MKVRPSIKKRSADCKIVRRKGKLYVINKKNPKFKQRQG"
     gene            9569..9949
                     /gene="rpsM"
                     /locus_tag="PLM90_02360"
     CDS             9569..9949
                     /gene="rpsM"
                     /locus_tag="PLM90_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMZ88239.1"
                     /translation="MARIAGIDLPKNKRGVIGLTYIYGIGDTTAAQILAAAGVDESKK
                     VKDWNDDEINAIRSYINNELKVEGALRSDMQLSIKRLMDIGCYRGIRHRKGLPVRGQR
                     TKSNARTRKGRKKTVANKKKAPKG"
     gene            9992..10390
                     /gene="rpsK"
                     /locus_tag="PLM90_02365"
     CDS             9992..10390
                     /gene="rpsK"
                     /locus_tag="PLM90_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMZ88240.1"
                     /translation="MAKTTSAKKTTKKRVVKVDAEGQAHILASFNNLIITFTNLSGQT
                     ISWASAGKAGFRGSKKNTPYAAQVACTDAAKVAYDAGLRRVEVFVKGPGSGRESAIRT
                     IVANGIDVSVIRDLTPMPHNGCRPPKKRRV"
     gene            10417..11022
                     /gene="rpsD"
                     /locus_tag="PLM90_02370"
     CDS             10417..11022
                     /gene="rpsD"
                     /locus_tag="PLM90_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMZ88241.1"
                     /translation="MARYTGPTTKKSRSFGESIFGYDKAFEKRKYAPGQHGLSRKKKS
                     ASEYGVQLKEKQKAKYTYGLLERQFRKTFEAATRRHGVTGEALLQLLEARLDNTVFRL
                     GIAPTRRGARQLVNHKHIEVNGQICSSPSATLRPGDVITIREKSKDHEWILAAANNTG
                     KRYSWLEWNPTQLKGVFVNYPERDQIPENIREQLIVELYSK"
     gene            11044..12042
                     /locus_tag="PLM90_02375"
     CDS             11044..12042
                     /locus_tag="PLM90_02375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HMZ88242.1"
                     /translation="MALLSFQRPDKITLQKATDFEGTFEFKPLEPGFGVTIGNALRRV
                     LLSSLEGFAISSVKISGVDHEFATINGVMEDITEIVLNLKQVRLKRELGGDENAVEKI
                     FISLKDKEEFKAGDIEKHTNSFRVTNPDLLICRMEPFVKLDIELTIQKGRGYLPAEEN
                     KNVDRTIGVIPIDAIYTPIKNVRYTIENTRVEQKTDYEKLLIEIKTDGTIHPEDAIKE
                     ASRILIQHLMLITDEHITFNTVGEKETNVVDEHTLHIRKLLKTPLEDLDLSVRAYNCL
                     KAAKINSLAELVRYDTNELLKFRNFGKKSLVEIEAMVLDKGLQFGMDLAKYNLDED"
     gene            12080..12580
                     /gene="rplQ"
                     /locus_tag="PLM90_02380"
     CDS             12080..12580
                     /gene="rplQ"
                     /locus_tag="PLM90_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HMZ88243.1"
                     /translation="MRHGNKINHLGRTTAHRKSMLSNMGTSLILHKRIKTTLAKAKEL
                     RVYIEPIINRGKEDTTHNRRTAFSELQSKEAVQELFTNISGKIAERNGGYTRILKIGG
                     NRVGDNAETCYIELVDYNENMLKTAKAGGAKKRSRRGSGKTEKKAVEPETKAQDAAEG
                     TSEEQA"
     gene            complement(12632..13522)
                     /locus_tag="PLM90_02385"
     CDS             complement(12632..13522)
                     /locus_tag="PLM90_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88244.1"
                     /translation="MHKSKQQGMTLTAYATLAAAVIPMVAPADVVYTDVDPDVLVNEP
                     GTGISLDLNGDGTNDFNFFFSSANSAVFTYFGGLNWYIINGIFAAPQNDNAIAAFTGS
                     GGAYAYPYAIGSGINIGPYAGQFLTNPYQTLAYQFYALIGSYLYYPIFAGGDWVFGQE
                     DKFVGLQLAVGDSTYYGWIRLTTDPSNRAFTVKDFAWENNANTSIETFLQPTAITNPE
                     LLEVEMYASGNNIIIHLNEDPKNAYLLNVYNLSGQLILQDNILQKDSRIMVDAPQGMY
                     IVELTGNNQKAGKKLFIAGT"
     gene            complement(13587..14513)
                     /locus_tag="PLM90_02390"
     CDS             complement(13587..14513)
                     /locus_tag="PLM90_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88245.1"
                     /translation="MKKSFTTGLSRKNLAAYSSMAGTFLLLHTSAQGQVGYTDLDPDE
                     TLNPLDVYDLDMDNNGDIDFTFNFTTFTLPDFFYTVAYHSVYFDGLFNVMKIYPTPGN
                     GVVAYPLSTTGGATLAYAEALNAGADIDGGQAFYTNSAIYMGAFLSVKDYPGVGSNYQ
                     FYTFGAWPGKTGKFVGVRFEGDGNEYYGWVRVSVNNDDLEITIDDYAYLSTPETAIEA
                     GQLPTAIHNIDPSQLNVYANGNIINIGVNELNTTSADVNVYDIQGRVVYSNGLNMSGM
                     QITLDAVATGTYTVKITTAEGANYAKRVLINQ"
     gene            complement(14638..15489)
                     /locus_tag="PLM90_02395"
     CDS             complement(14638..15489)
                     /locus_tag="PLM90_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88246.1"
                     /translation="MKKLYNSKNAGKLAAYSAMAGAFVATTSEANATVVYTDIDDVTV
                     ELGETYALDLDNNGYSDFLMAAVSNSAGNWTWAYMIGNLSAYGYGGGSNMVIGYTGPI
                     LSYGSALNDGDLIGPDASFVSNTYNMLWLASIYSGVTYGPFANTEDKYIGVQFDIDGS
                     LHYGWARLDVSVGPVSFTIKDYAYDDAEGTDISAGEKDVAINNLTEAQVAAYSYGNTI
                     NVVIKDSEAGVDNVNVFDIDGKLVYSARLTGSNMAISLDNAATGLYTLQLTGANTSFS
                     KSLYIQN"
     gene            complement(15607..16491)
                     /locus_tag="PLM90_02400"
     CDS             complement(15607..16491)
                     /locus_tag="PLM90_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88247.1"
                     /translation="MKKDFTSTKKLLQYAASATAFLAINQAGATVVYTDLDPDLAIGG
                     EGAEASIDINEDGTDDFTFSIYNFSGSGTYSGFSFGYVVNIALGSALNGNEFLGSIVS
                     YSGYSGVYAPILPAGTLVGSDDNFANGAMSMAVSASVTVLGFPYYTYVGGNWLDTDMA
                     FMGFRINIDKDRYYGWMRVSVSDDATMITIHDYAYQSEPNTAIFVGQTATEIEENPLS
                     NAQIFSNGGNIVVDIPSDFTGTADLQLFDMNGKLVKTMSGLSGHNVLDCNDLASGNYA
                     VRLGNAELTVKKQLNLSI"
     gene            complement(16583..19141)
                     /locus_tag="PLM90_02405"
     CDS             complement(16583..19141)
                     /locus_tag="PLM90_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ88248.1"
                     /translation="MGKRIAKKVLLIGWDAADWKVINPLMDAGLMPTLEKLVNGGVIG
                     NHATLDPPLSPTLWTSIATGKRPYKHGIIGFTEPDPSGKGIRPVYITSRKVKAVWNIF
                     NQHNLKSHVIGWWPSHPAEPINGTMISNLYQRATKPINEPWPMLDGTVHPADKAELFK
                     KMRIHPAELTASHILPFIPDANKIDQEKDKRLGMLTKIIADCSTIHNAATYILENEEW
                     DFLAVYYDAIDHFCHGFMKFHPPQMPMVPDDLYNLYKDVVAGGYKYHDMMLERLLELA
                     GPDVTVMIISDHGFHPDHLRPIELPKEPAAPALEHSPYGIFVLNGPGIKKDERIYGAS
                     VLDVTPTLLTLFGLPVAADMDGNVLVNALETPVIPDKIDTWEDIPGDSGQHPADKQED
                     PYAAAEAMEQLIELGYIERPSENIEKAIKSTVSENNYYLARSYMNGRKYAEAVDILKK
                     LVDENPGTNRYAMRLAHAYQSMNKVEETREVLSKIKTENNGRESVSMKIMEGNLLLVE
                     NKPKMAMELFEAIEKEMPNAARVHMQLGRCYMALKRWKKAEGAFLKELEHDPGNASAH
                     HGLGITLLRRKHYEESIDCFLNAVGLTYFFPFAHYHLGEALYYLGEYERSAEAFEVCL
                     KMAPGINKARQLLSTIYSEHLKKPEAAVEVKSQIKDYIKGDIVVVSGLPRSGTSMMMQ
                     MLDKGGLEIFTDGLRTPDENNPKGYYEHELVKSLARNKTWMKDAMGKGVKVISHLLFE
                     LPANYRYRIIFMERDLDEVIKSQHHMLVRDGKAKEDALNLRIVNAFKQNLERVQTWVP
                     QQQNMEILYVSHRNLVNNPTEELKKINAFLGGTMNVDAMAAVVDKSLHREKSGK"
     gene            complement(19196..19405)
                     /locus_tag="PLM90_02410"
     CDS             complement(19196..19405)
                     /locus_tag="PLM90_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88249.1"
                     /translation="MEKKQGNKLRITTQDGKPFRIPVEGSLGLLALGYKGLIAWRQAK
                     WQFQKMKEAKQGGNTEQQQDEPIAD"
     gene            complement(19531..22629)
                     /locus_tag="PLM90_02415"
     CDS             complement(19531..22629)
                     /locus_tag="PLM90_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ88250.1"
                     /translation="MRKLTAFLSVICLSSILPAQLVVTPDTTICAGGTATLAVISSPS
                     YGTSSYTFEVYPYSPETYAGTSPIQAGGGGLTDDSYSTAVPLGFTFCFLGNQYTQCYI
                     GSNGWVSFGGPGALVTTFTSAAIPSVAGTVPKNCIMGPWQDWHPGLCTPVGSCIKYQT
                     IGTAPNRKFVVSWDNVPMFSCTGLYGKFQIVLNETTNVIENHLTNKPNCMAWAGGTAT
                     QGVHSGDGLTAFTAPGRNSTPWTTTNESTRFVPNGIIWYDPAGAIVGYGDSLIVSPTV
                     TTTYTAFLESCSGTDYTADIVVTVDDPDPSFSYDPFYCTTGYAMPTVTGDPGGTFAAV
                     PAGMVIDPVTGEIDLNASTPGSYSVSYTAYGITGDCPSETAVDLVTIVVDPDASFGYS
                     DINYCPTGTTTPTFITTAGGTFSVSPAGLTVNPTTGVVNLTTGTIGTTYTITYTVGIL
                     CVSTYSVTITITDFDDPSVNYPAPSYCPTGTATPTVATPGGTYTVSPAGLVVNPTTGV
                     VDLTTGTVGTTYTITYTTPTGPCSNSTTTTITIDPLDDPTFSYSAPSYCPTGTTLPTS
                     ITTPGGTFSIFPTTMGINSTTGMLNLASGDIGTTYTITYTTPAGPCSNSSTETVTIDP
                     LDDPGFTYDAASYCNYGTAIVTSVETPGGNFTVSPATLSINALTGAIDLTTGIPGSFY
                     TITYTTPAGPCSNSTSVVIEIMPLTDAAFSYLLPAYCATGTTLPNMILNPGGTFSAMA
                     GISINAATGELNLAACTPGGPYEIYYTSPGCPEVDTVEVTIYPLPTPTIDLIDVICLE
                     GDPTLIIGDPAGGTFSGEGVIGDEFDPALAGDLGLYTVTYTYTDANGCTNNTTGAINV
                     IQNDVDAGIDQYIPEYTTTVITATGGTTFLWEPPAGLSCTDCPAPVFDSLLSQTYMVT
                     SWDAFGCIDSDMVTITVVPVFDPVVFVPNTFTPNGDNINDYFFAYGTDLASIVSMNIY
                     DRWGELIFTKENMIAGNPSAGWDGTYNEEPVAQGVYAFMLIVQLEDGMKQHMQGNITL
                     IR"
     gene            complement(22767..22840)
                     /locus_tag="PLM90_02420"
     tRNA            complement(22767..22840)
                     /locus_tag="PLM90_02420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22804..22806),aa:Asn,seq:gtt)
     gene            22967..23887
                     /locus_tag="PLM90_02425"
     CDS             22967..23887
                     /locus_tag="PLM90_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMZ88251.1"
                     /translation="MSLLVVGTVAFDAIETPFGKRDKVIGGSATYIGLSASYYYKPIH
                     IVSVVGSDFPKATLAAFNKKGITTDGVQVIKGQKSFFWSGKYHMDMNSRDTLRTDLNV
                     LADFNPVLPEKLRKPNMLMLGNIDPNLQIRVIEQLKSRPKLIAMDTMNFWMDIARPAL
                     DKVLKMVDVLIINDEEARQLTGEYSVVKAAKKIMKNGPKTLIIKKGEHGALLFSGSEV
                     FFAPALPLEEVFDPTGAGDTFAGGFMGYLSQSKTLSFTNMKGAIVYGSAMASFCVEEF
                     GPEKLLALKKPEIAKRVKMFSTLSKFSIKG"
     gene            complement(23877..24122)
                     /locus_tag="PLM90_02430"
     CDS             complement(23877..24122)
                     /locus_tag="PLM90_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HMZ88252.1"
                     /translation="MDVNTLPAYNKFQLALRNGQDKEEIWIAYKGLIYDVTPSRLWRN
                     GKHYEHFAGQDLTDELKDAPHTEITLQKFNIIGRLIP"
     gene            complement(24113..24814)
                     /locus_tag="PLM90_02435"
     CDS             complement(24113..24814)
                     /locus_tag="PLM90_02435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88253.1"
                     /translation="MEPDIKIEYLDWDSRFFGFKIGRLELQSTCIITEHQVGNWRSEG
                     YTCLYIDSATEVEISNRSSATLNPMSSRCLLQRETRAPQLNPEVKILTQVTDGIIELG
                     IHAGKHSRFMCDDHFNKKKTTALYAMWIRESLHGKMGDEIYFIGTEINPLGLIAFTNK
                     KSHMQIGLLAVAPGMRNQGIAKKLLTASDHFANAYQLPVMNLYTQGNNIAAQHLFAQD
                     GFTQISSINQFHLWM"
     gene            24873..25781
                     /locus_tag="PLM90_02440"
     CDS             24873..25781
                     /locus_tag="PLM90_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ88254.1"
                     /translation="MLTISNLTKSFGDHVAVDGISLSIAKGMFYGLLGPNGAGKTTTI
                     QMISSILPPDSGKISVDGLGVYAHASVKMHMGIVPQEIALYDELTALENLIFWGNLYA
                     VHGKTGKDRAAFLLDWVGLADRRHDIVKNYSGGMKRRVNIAAALMHDPGLIVMDEPTV
                     GIDPQSRNKIYELLDELHHQGKTILYTTHYMEEAERMCDKIGIIDKGKIIAEGSLDEL
                     KKAHAQEENIVIHFTGEAVSKLGNYVISFPEPQMMTVVTPQARKRLHDVVQLCTEGGI
                     DITGIDIHDAGLESIFLNLTGRQLRD"
     gene            25792..27003
                     /locus_tag="PLM90_02445"
     CDS             25792..27003
                     /locus_tag="PLM90_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88255.1"
                     /translation="MWTIAKKDIYIFFKDKRGLFLSLLLPIGLITMFAFAFGGIGQDD
                     EDPAPIHVLYVDADQTTSSKSLIAMLDTIPGIACLSTTAEDATANIKSGDEMAAVVIN
                     KGFEMALQTGEDLPVVLQYDQSRVMEIGVLQQLLTSRLSAMKGSVDAHRGINKIMQQS
                     FSNMPPNVQDSIRTSLEENLLQKENHEQLISMQEVVGDAESNWGLIQAVAGTAIMMLL
                     FSVSSIGKSMLDEKESGVLRRLLQTPMHPYGLLFGKMITALVIAVFQLTIMFLFSWLA
                     FGLDIFINIPALIIMILATSLACSAFGVLLASLVTTRKQADSLSTILILFMSAVGGSM
                     IPLFIMPVFMQNAAVVSVNYWSIQGFYDIFWRQAGLEAIVGNALVLTGISAGVLLISM
                     YFYKKNILRLI"
     gene            27029..28564
                     /locus_tag="PLM90_02450"
     CDS             27029..28564
                     /locus_tag="PLM90_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88256.1"
                     /translation="MKRILLSCIIFSGFIASAQDALDPSFGGDGIVTLDFGGNDIAYA
                     VLIQPDGKILAAGQAGIGPALTTDIAIARYNTDGTLDPVWGGDGTVTLDLGVDYSDFS
                     FALARQPDSKIVVAGCGFFDSGYKFAVLRYRADGSLDPAFSDDGIFITDFGGMDDWAR
                     AVYVMPDGKIVASGYRNDGLKHNFAIIRLTPEGDLDPTFGTGGKVYTVFPVNRHDQSY
                     ALAVQPDGKMILAGYSYVGTTADYQFALARYNEDGSLDDTFGDAGLVLTPVPMIQGES
                     YAMELQPDGKILVSGFSYTDWKTDIAIIRFNSDGSVDTEFGTDGLVKTDIATDRDDHA
                     YAMYLLADGRFLVGGNRWNGYDWDMALLRYNADGSLDNTFSGNGMLTIAIGDDADCIQ
                     AIDVQSDGKIVVAGYSDNGPNNDFALVRFMQDGGSGVGISNQVVTANFSISPNPGYDN
                     ISINCTLSGIIDIVDMQGHVVERREVVSGDNTIDIHQLPTANYIIRWRNESAQCSLPF
                     IKK"
     gene            complement(28573..29040)
                     /locus_tag="PLM90_02455"
     CDS             complement(28573..29040)
                     /locus_tag="PLM90_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ88257.1"
                     /translation="MDIIINIIAFLAAFAFMEAFAWFMHKYIMHGVGWFLHRSHHEPR
                     HGRFELNDLYAIIFAAPAIYLMYIGSPAYNWIFWVGTGISAYGLCYFGFHDVLVHRRM
                     KHHMRPESKYMQRIIRAHKIHHKKLGRKDGEAFGFLFALPKYDVKKTNADNEE"
     gene            complement(29076..29795)
                     /locus_tag="PLM90_02460"
     CDS             complement(29076..29795)
                     /locus_tag="PLM90_02460"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HMZ88258.1"
                     /translation="MNQSYLYLLINTGTILFPFLFSFESRVRYVNWWKWLFPSMFITA
                     LVFLTWDQIFTVNGVWGFNDRYITGAHFMNLPIEEILFFFTVPYASIFIYAFTNTIWP
                     DTPTLDKYANKISYAMLTIALILLVANYNKAYTALNCGYAVVLLCIQLFLVKGTYMGK
                     FYRFYLWHLIPFFIVNGILTGWGIAEEVVWYNNHENLGIRMGTIPIEDSLYSFSLMLM
                     NIMLIEGFKSRSKKKNALIGA"
     gene            complement(29792..30637)
                     /locus_tag="PLM90_02465"
     CDS             complement(29792..30637)
                     /locus_tag="PLM90_02465"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HMZ88259.1"
                     /translation="MLHLFHESSFTCSRKVTELYSTSFTLGIKALDRRYHDAIYAIYG
                     FVRYADEIVDTFHDWDKQTLLLRFKEDTWSALDEKISLNPILHAFQLTVHKYQIDRNL
                     IDAFLHSMEMDLHMSNYKQHHYEEYIYGSAEVVGLMCLKVFCENDETHYQKLIQPARF
                     LGAAFQKVNFLRDLQSDYKDRGRVYFPNVDFENFCERQKTQIEQEIANDFDEAYKGIK
                     QLPEGAKFGVYLAYKYYLELFKKIRSTEPEAVMQTRIRVSNPKKIGLWASLYLRSNVL
                     GIYAP"
     gene            complement(30692..32173)
                     /gene="crtI"
                     /locus_tag="PLM90_02470"
     CDS             complement(30692..32173)
                     /gene="crtI"
                     /locus_tag="PLM90_02470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HMZ88260.1"
                     /translation="MAERKHIIVIGAGFSGISAACALAAKGYSVTILEKNESAGGRCS
                     VFSEQGFTFDMGPSWYWMPDVFEWFFNTYGSSVEQQYALKRLDPGYRIVFGPGDVMEI
                     PASTSELKALFESIEPGSGIQLQKFLDDAAYKYEVGMKDLVFRPCNSIMEFAELRMVK
                     ALFTMQLFTSLRSEVYAKFKNERIRKILEFPVYFLGALPSNTPALYSILNYADLVLGT
                     WYPMGGMYKIIEGMQRVAEGLGVQFQFNSPVEKIIVEEGRSTGVKVHGDFIAASGIIA
                     AADYHHVEQKLLDQPYRNYDETYWDKKIFAPSAIIYYLGVKGRVKNLLHHNLFFDQSF
                     EQFADEIYTDPRWPEQPLFYVSAPSVTDPTVAPAGDENLFILVPVAAGLKDTAELREE
                     YYHKAMDRLEAHCGEEIKSRVVYKREFAPGNFVDRYNACKGNAYGLANTLMQTAIFKP
                     KMRNKRVRNLCYAGQLTVPGPGVPPSLISGYVAAQEMEKLVGH"
     gene            complement(32229..32531)
                     /locus_tag="PLM90_02475"
     CDS             complement(32229..32531)
                     /locus_tag="PLM90_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HMZ88261.1"
                     /translation="MYLYNVTIKIEQSRADEWLTWMREKHIPDVMATGFFVEHRLCRL
                     VDDGDMEGMTFVVQYTLNNLDDFLTYKLTSGPSLQRDTQEKFGNDFFAFRTLMEII"
BASE COUNT         9790 a         6565 c         7466 g         8983 t
ORIGIN      
        1 gaaaactgta gaatacgatc caaaccgtac tgcgttcatc gcattgcttg tatatgctga
       61 tggtgagaag cgttatatcc tcgcaccaaa tggtatccag gtaggccaaa cagtacagtc
      121 aggcctgaat gctgctcctg aattgggaaa tgcattgatg ctccgcaata ttcctctggg
      181 tactatcatc cacaacatcg aactctatcc gggtcgtggt ggtatgatcg ctcgcagtgc
      241 aggaagctgg gcacagttgt cggcaaaaga aggtaaatat gttatcatcc gtatgccttc
      301 aggcgaaaca cgtatgattc ttgatacctg ctttgcaact gttggaacgg tatctaatcc
      361 tgaccacaac ttacagcaac tcggtaaagc cggtcgcaaa cgttggctcg gtcgtcgccc
      421 acgcaaccgc ggtgtggcca tgaacccagt cgatcaccca atgggtggtg gtgaaggacg
      481 ttcttcagga ggtcacccac gttcacgcaa cggtcagatc gcaaaaggtc tgaagacacg
      541 ccaaccgaag aagacaacat ctaagttcat tattagcaga cgtaaaaaat aattctataa
      601 gtaatggcac gttcgattaa aaaaggacca tacgtagatc ataacctggc cggtaaagtg
      661 gcacagatga atgaatcagg caaacgcact gtgatgaaaa catggtcgcg ccgttctacc
      721 atcctgccgg aaatggttgg ccatactttc gcagtacaca atggaaataa attcattcct
      781 gtgtatgtga cagaaaacat ggtaggccac aaattgggtg aattttcacc aactcgcaac
      841 tttaaaagtc acgctggaaa cagataatta ttatggaagc agtagcaaaa ctcatcaaca
      901 acccaagaac gaacaacaac tcaggttcgc cgcgtaaggc tcgctatgtt gttgacatga
      961 tcaggggcct cgatatcgat aaggcattga gcgtgttgaa atatacaaac cgtcgcaatg
     1021 taactgcagt ggaacaaaca ctgcgcagtg ccattaagaa ctgggaaagc aaatacgaaa
     1081 tggatgctgc cgacagcaat ttgtatgtga aagaagcatt tgtagatggt ggaaaaacca
     1141 tgcgtcgctt ccttcctgca ccacatggtc gtgcttaccg tatgcgcaag cgtactaacc
     1201 acctcacaat tgtggtagca tctcgaaatg caaacaatca agaaaaaaac gcataacaat
     1261 ataactgatt cgaatgggac aaaagtgtaa cccgataggc aacaggttag gtatcatccg
     1321 cggatgggac agcaactggt atggcggcaa aacaatggcc gagaaactgg ttgaggatga
     1381 aaaaatccgt cagtacctga atgcccgtat ctcgaaaggt ggtatcagcc gcatcgtaat
     1441 tgagcgcacg ctgaatcgta tcaccgtaac aattcatacc tcacgccccg gtatcattat
     1501 cggtaaaggt ggtggtgaag tagataagct gaaagaagag ctgaaaaaaa tcacagaaaa
     1561 ggaagtacag atcaatatta ttgagatccg tcgccctgaa ctggatgcca acatcgttgg
     1621 cgaaacaatc gcacgccagc ttgaagcaag gatcaatttc cgtcgtgcca ttaaaatggc
     1681 tgttgcttca accatgcgca tgggtgccga aggagtgaag attcgcgtga gcggtcgcct
     1741 caacggtgct gagatggcaa gaactgaaga atacaagcaa ggacgtgtgc cgctgcacac
     1801 cttccgtgcc gatatcgatt actcatggaa agaagctcat accgtatacg gaaaaatcgg
     1861 tgtgcgcgtg tggatctgta aaggtgaagt actgggtaaa cgcgacctgt cgccaaacgt
     1921 aggtatgtcg caagaaaaag aacgcagcaa acaaggaaga agaagaccga ataaacgata
     1981 aaatagaacg caatcatgtt agcgcctaaa aaagtaaaac atcgtaagac attcaagggt
     2041 aagatgaagg gtgatgccaa aaaaggctcc caactttcca ctggatcctt cgggctgaag
     2101 acactggaat caggttggat taccgaccgt cagatagaag cagcaagggt ggctttgacc
     2161 cgtcacttga aacgtgaagg tcaggtttgg atcaacatct ttcccgataa gcctatcact
     2221 aagaaacctg cagaggttcg tatgggtaag ggtaaaggta accccgaatt gtgggtggct
     2281 gttgtaaagc cgggcagaat tatgttcgag gcagaaggtg tgccactgga tactgcacgt
     2341 gaagcaatgc gtcttgcagc agggaaatta ccggttaaaa caaaatttgt agttcgcaga
     2401 gactacgtag cataattaaa aaaatagcaa tggctaagtt aaaacgagaa gatttgaatc
     2461 agctcagcaa tgaggacctg caacaacgct tgcatgatga ttcacagcac ctcacaaagt
     2521 tgcaattcac acatgcagtg aacccaatcg acaacccgtc gcagttgcgt tcaatccgca
     2581 gagagattgc aagaattaaa actgagatac gcaaacgaga aattgctagc gccaaataaa
     2641 ataaataggc atggaacaaa gtaccagaaa gttaagaagg accattatcg gtgtggtgac
     2701 cagcgacaaa atggataaat ccattacagt tactgtggaa cgtaaagtga tgcacccgaa
     2761 atacggtaag ttcctgaaga aatcaaaaaa ataccatgcg catgatgaag caaatgatgc
     2821 taaatcaggc gacctggtga aaatcatgga aacccgtccg ctgagtaagc tcaaaagatg
     2881 gagattagtt gacattgtag aaagagctaa ataacatgat ccagcaagaa acaagattaa
     2941 aagtagctga caacagcgga gcacgtgaag tattgtgtat ccgtgttttg ggaagcacgc
     3001 gcaggagata tgcatcgctc ggcgacaaga tcatcgtaac ggtgaaagat gcactgccgg
     3061 gtggcggtat caaaaaaggt actgtttcaa ctgcagtagt agtacgcaca cataaagaag
     3121 tgcgtcgcaa agacggaagt tatatccgtt tcgacgacaa tgcatgtgtg ttgctgaatg
     3181 catcagacga accgcgcgga acacgcatct tcggacccgt agcccgcgaa ttaagggata
     3241 aacaatttat gaaaatcgtt tcactggcac ctgaggtgtt ataactatga aagaaacaaa
     3301 ttacagcaat cgcttccagc caaagtatca tgtgaaaaag aatgataccg tagtggtgat
     3361 cgccggtgcc gataaaggca aaaaaggccg tgtgatagaa gttgactacg ctaaaaatcg
     3421 cgtaatggta gaaggcgtga acatcgtgtc taaacacaca cgtcctactc aacaatatcc
     3481 taatggtggt atcatcaaaa aggaagcacc aattcacatt agtaatgtga tgcccgtaga
     3541 tcctaaatcc ggaaaaggca cccgcgttgg ccgtaaagag gaaaacggaa aatcagtaag
     3601 atattctaaa aaatcaggag aaatcattaa gtaatggaaa ctaagagccc gcgtcttaaa
     3661 aagcaatacc atgaaacggt ggtgaaggac ttacaggaga aattccagta taaaagttct
     3721 atgcaagtac ctaaactgtt gaagatcgca atcaaccaag gtttaggcgc tgctacccaa
     3781 gataaaaaag tggtagacgt agcactcgac gaattgacgc gtattaccgg tcagcacgcc
     3841 gtgtcgacaa tttctaaaaa ggcgatctcg aacttcaaac ttcgtgagaa tatgccaatc
     3901 ggtgcacgcg taacacttcg tggtaacaat atgtatgagt tcctcgacag attgatttct
     3961 gttgcactgc cacgtgttcg cgacttcaaa ggcgtaaaca acaaaagctt cgatggtcgt
     4021 ggaaatttca cactgggtat cactgaacaa atcatcttcc cggagatcga tattgataaa
     4081 gtaacaaaga tcaccggtat ggatatcaca tttgtgacca cagcaactac cgatgctgaa
     4141 gcttatgaat tattaaaagg attgggaatg ccattccgca aagcataata taaacgcatg
     4201 tccaagaaat cagtaatagc aagaaataac aagcgcgagc aaatgatcat caaatttgct
     4261 gagcgtcgca agcaactgaa agccgaaggt aactgggaag ctctagataa acttccacgt
     4321 aacgcttcac cgatacgcta caaaaatcgt tgtaagctta ccggtcgtcc acgtggttat
     4381 gttggtgact tgggaatttc caggattaaa ctccgcgacc ttgcattgta tggcaagatc
     4441 ccgggcatga caaaagcaag ttggtaatcc aaagcagtaa aaaaagcaag agatgaaagt
     4501 aacagatcca atagcggatt ttctgaccag gatcagaaat gcccaaaagg caaaccaccg
     4561 tatcgtggaa ataccgtcat cgcaaatgaa acagcgattg acagagatcc tgtatgaaaa
     4621 cgggttcatt tataaatata agttcgagca ggaaggtcct cagggaacta tcaaaatcgc
     4681 acttaaatat gacccggtaa caaaatcgcc tgccataaaa ggcatcgagc gtgtaagtag
     4741 cccaggtttg cgtcgctaca caggtgtgga tagcctccca cgtgttatga gcggtctcgg
     4801 actggccatt atgtcaacat caaaaggtgt aatgacagaa aaagaagctc gtcgccaaaa
     4861 tgttggtggt gaagtattgt gttatgtgta ttaattaata aagaaagcag atcatcatgt
     4921 cacgaatagg aaggttaccc atccaattac ccgccggagt tacactaagt gtggctggta
     4981 acaatatggt aacaattaaa ggaaaacgcg gtgagttgac cttgcaggtt gacccggata
     5041 tcacggtgaa aactgaagac aacgctatta aattagagcg tccgtcagag caaaaacgcc
     5101 atcgcgctat gcacggttta taccgcgccc tcattgccaa tatggttaaa ggtgtttccg
     5161 atggattcaa gaaagaactt gaattggtag gggtaggtta cagggcagcg aatacgggcc
     5221 agatcctcga gatctctgtt ggatactctc acccaatcat gatgatgata cctaaagagg
     5281 tgaaattatc taccgtgagt gaaaaaggta aaaacccaac tatcatactc gaaagcaacg
     5341 ataaacaatt gcttggagcg atagctgcta agatccgttc attccgtaaa ccggaaccat
     5401 acaaaggaaa aggtattaaa tattctgacg aagtattgcg tcgtaaggca ggtaaaactg
     5461 ccggtaaata atcggtaaaa ctatgttgag caaaaaatca attaggagac aaaaaattcg
     5521 ctaccgtatc cggaagaaaa ttgccggaac ggccgagtgt ccgcgcctgg cggtattcag
     5581 gagcaacaaa gagatctacg tacagttgat cgacgatgtt gcaggccaca cattgtgcgc
     5641 tgcttcgagc aatgacaaac aaggtaaagc ttataccggt actaaggtag aaaaagctgc
     5701 tgccgtggga aaacaaatcg ctgctcaggc acaagcaaaa ggcatcacca ctgtgatttt
     5761 cgatcgcgga ggatatttgt atcatggacg cgtgaaatca cttgcagatg gtgctcgtga
     5821 aggaggtctc caattctaaa aatgcatttt atctatggca gttaataaag atatacaaag
     5881 agtaagggcc agcgagctcg aattaaaaga gaagatcgtt ggtatcagcc gcgtagctaa
     5941 agttaccaaa ggtggtcgta cattctcatt ctctgcactt gttgtagtgg gtgatggaaa
     6001 cggaaccgtt ggtcatggtc tgggaaaagc acgcgaggta accgaggcta ttcaaaaagg
     6061 tattgatgac gctaagaaaa acctcatcaa agtaccggtg ttcaacggaa ctattccgca
     6121 cgaacaacac ggtaaatttg gtgccggcaa agtgatgatc aaaccagcag ctcatggaac
     6181 gggtgtaatt gccggtggtg caatgcgcgc agtacttgaa agtgccggag taaccgacgt
     6241 attggcgaaa tctttgggat catcaaatcc gcacaacgtg gtaaaagcta ccctcgatgc
     6301 acttgctcac ctgcgcagcc ctctggctgt tgcacaacat cgcaatgtgt cagtgaaaaa
     6361 agtatttaac agttaataat aaccgtgtaa gctagcgata tggcaaagat taaaattaca
     6421 tctgtcaaaa gcacaaccga cagaccaaaa aaccagaagg ctacaatgaa ggccttaggt
     6481 ctgcgcagaa tgcatcagac cgtggaggtg gaagccaccc cacagatcat tggcatggtt
     6541 agagtagtac agcatttggt aaaagtagaa aacgtataac atggatcttt caaacttaaa
     6601 accggcttcc ggttccatca agaagcgcca tcgtattggt cgcggacaag gttccggctg
     6661 gggtgaaacc tcgaaacgcg gtttcaaagg tgcaggctca cgttcaggta catccactaa
     6721 acgaggattc gaaggtggac agatgccatt gcaacgccgc ctccctaaac gtggattcaa
     6781 taacaagaat ttcgaaacga aatatgttac cctgaacctc gatcaaatcc aggaaatggt
     6841 cgataaatat gagatcacta aactggatca ggatatgctg gtcgcaaaca gaatcatcaa
     6901 aggtgatgat aaactgaaag tgctcggcag aggcgaatta aaagcgaaag tggaaatctg
     6961 ggctcatgca tgcagcgaaa cagcacaaaa agcaatcgaa gctgccggcg gtaccattac
     7021 tatgatcgga taacacatga aacaatttat tcaaacatta aaaaatatct ggagcatcga
     7081 agatctccgc aagcgtatca tctacagcct tggtctcatc ctgctgtatc gcttcggttc
     7141 ttacgtgatg ttgccgggta ttgatgcagg catctacaac caggctgcta acgtagatag
     7201 tcagagcggt ttgctcggat taatcaactt gtttgcaggt ggtggtttta gccgtgcctc
     7261 catttttgca ttaggtatca tgccttatat ctcagcatct atcgcaattc agttgctggg
     7321 tattgcagtt ccttatttcg ctcgcctcca aaaagaaggt gaaagcggac gccgtaagat
     7381 gaatcagtac acgcgttatc ttaccgtgat agttaccgca ttgcaggcat caggatatta
     7441 tgcatacctg aacactatgt ctaaaaatgc cgacttcatc gtggcacctc aaggcttgtt
     7501 ctggttcatt acagtggtga ttcttactgc aggaacctta ttcacaatgt ggatgggtga
     7561 gcgtatcact gataaaggaa ttggtaatgg tatttccatg attatcatgg tgggtattat
     7621 tgccggactt cctgctgcat tggtgaatga gattacacat cgtggcgctg cttccggcgg
     7681 tggatttatt ccattcctgc tggagattgc attcctgatt gtagtgatca tgggttgtat
     7741 tttgcttgtt caaggtacaa gacgaattcc tgttcaatat gctaaacgag tagttggatc
     7801 tcgtcaatac ggtggcgtac gtcagtatat tccattgaaa ataaatgctg ccggtgtaat
     7861 gcctatcatc ttcgcacaag cattgatgtt tattcccggc ttattcgcac aaataaatga
     7921 agattggcag tcatcaccgg tattggttat gctgaacgat tttcaaagtt taggttataa
     7981 tttgatttat ggtttcttga ttgtattgtt tacgtatttc tatacggcaa tcatcatcaa
     8041 cccatcacaa attgcagatg atcttaaaaa gaatggtgga tttattccag gaattaaacc
     8101 cggaaagaaa acatcagaat ttatagataa cgttttatcc agaatcactt tgccgggatc
     8161 tattttcctt gcactggtag cgatatctcc tgtgttgaca cgtgcttttc atgtagaccc
     8221 ttcatttgca aggttctttg gtggaacatc attgctcatc atggtaggtg taattttgga
     8281 tacattacaa caaatagaaa gtcacttgtt gatgcgtcac tacgacggat tgatgaaatc
     8341 cggtcgtatt aaagggcgca caaacgcacc cgttagcata caacagggat gatttattat
     8401 aaaacaacag atgagataac actcatcaga aaaagttctt tacttgtttc agaaacgctg
     8461 gcaatgatag ccggcatcat caaacccggt gtaacaacat tgtggttaga tcagcaagcg
     8521 gaagctttta ttcgcgacaa tggtggtaaa ccatcattta aaggatataa aggatatcag
     8581 catacgctat gtgtttccgt aaatgatcag gttgtgcacg gcattccgtc ggcacgcgaa
     8641 atcaaaaacg gtgatgtagt atcggtagat tgcggtgcgt ttctcaatgg ttttcacggc
     8701 gactcggctt tcaccttttt agttggtgaa gtgagcagag aagcggtgaa tctggcaagg
     8761 gtgacaagag cctgtgtata caaaggcatc gagtttgccg tagccggaaa tcgcgttggc
     8821 gatgtgagtc atgccattca actgtatgcc gagcatgtaa atggttacgg cgtagtgcga
     8881 gaattggtag gacatggtat cggaaaaaac ctgcatgaag aacctgaagt gccgaatttc
     8941 ggagcgaagg gaaaaggtcc taaaatgcag gaaggcatgg tgatagccat tgaaccaatg
     9001 atcaatcttg gatctcggaa tgtggtgcag gaaaatgatg gatggacaat acgcacgcgc
     9061 gacggaaaag tttcttgtca ttacgaacat accgtagcag tgatgaaagg aaaggcagat
     9121 gtgttgtcga gtttcgaaat cattgatgaa gcaatggcaa agaacagtaa tttgtataca
     9181 gaaaaagaaa tagtaggtta atgtcaaaac aggacttaat aaaacaagac ggcacggtaa
     9241 cggaagcatt gtcaaatgcc aactttcgtg tgaagcttga aaacgaacac gaaatattgg
     9301 caacaatttc cggcaagatg aggatgaatt atatccgcat attacccggt gatcgtgtta
     9361 cagttgagat gagtccatat gatttaacaa gaggaagaat tatttatcgt tataaataaa
     9421 ccggcattat gaaagttaga ccgtccatta aaaaacgcag cgctgattgt aagatcgtgc
     9481 gcaggaaggg gaagctgtat gtgataaaca agaagaaccc taaattcaaa caacgtcaag
     9541 gttaatttta aattactgaa taaacaatat ggcaaggatt gctggtattg acttaccaaa
     9601 aaacaaacga ggcgtaatag gccttaccta catctacggc attggtgaca ccactgcagc
     9661 gcagatatta gctgctgccg gtgttgacga aagcaaaaag gtgaaggatt ggaatgatga
     9721 cgagatcaac gcgattcgtt catacatcaa caacgaattg aaagtagaag gcgcactgcg
     9781 ttctgatatg caattgtcta ttaagcgatt gatggacatc ggttgttatc gtggaattcg
     9841 tcaccgtaaa ggattgcctg ttcgcggaca acgtacaaaa agtaatgcac gtacgcgtaa
     9901 gggtaggaag aaaacagttg ctaacaagaa aaaggcacct aaaggataat ccaggcaatt
     9961 tgccgtttca tttcatttat ctctgtatac aatggctaaa acaacgtcag caaaaaagac
    10021 aacaaagaag cgtgtggtta aggtcgacgc ggaaggacag gcacatatcc tggcttcttt
    10081 caacaatctg atcatcacct tcactaacct cagcggacaa acaatcagct gggcaagtgc
    10141 cgggaaagcg ggtttccgtg gttctaaaaa gaatacacca tatgccgcac aggtagcttg
    10201 taccgatgca gcaaaagtgg catacgatgc cggtttgcgc agggtggaag tgtttgtaaa
    10261 aggccccggt tcaggccgtg agtctgcgat ccgcaccatc gttgccaatg gtatcgatgt
    10321 atctgtgatc cgcgacctta cgccaatgcc gcacaacggt tgccgtccgc ctaaaaagag
    10381 gagagtttaa tcaatcactg aaaatcaact agatcaatgg caagatatac cgggccaact
    10441 accaaaaaat caaggtcgtt cggcgaatca atcttcggtt atgacaaagc gttcgagaag
    10501 cgtaaatatg ctcccggaca gcacggtctt tcacgcaaaa agaaaagtgc gagtgaatac
    10561 ggcgtgcagc tgaaagaaaa acaaaaagca aaatatactt atggtttgct ggaacgtcaa
    10621 ttccgcaaaa cctttgaagc tgcaactcgt cgccacggcg ttactggtga agctttattg
    10681 caattgcttg aagctcgcct cgacaacacg gtgttccgtt taggaattgc tcctactcgt
    10741 cgcggtgccc gtcagcttgt aaatcacaaa cacattgaag tgaatggcca aatttgcagt
    10801 tcaccatcag caacacttcg ccccggtgat gtgattacta ttcgtgagaa atctaaagat
    10861 catgaatgga tattggctgc tgcaaacaac acaggaaaac gttattcctg gttggagtgg
    10921 aatcctactc aattaaaagg tgtgtttgtt aattatcctg agcgcgatca gattcctgag
    10981 aatatcagag aacaacttat cgtcgaactc tattctaaat aatgttaaaa actatctgac
    11041 catatggcat tactttcatt tcagcgtcct gataagatta ctttacaaaa agctacagac
    11101 ttcgaaggta catttgaatt caaacctctc gaaccgggct ttggcgtaac tatcggcaac
    11161 gcattacgca gagtgcttct tagctctctg gaaggttttg cgatctcttc tgtaaagatc
    11221 agtggtgttg accatgaatt cgctactatc aatggcgtta tggaagacat taccgagatc
    11281 gtactcaatt tgaaacaggt tcgcctgaaa agagaattgg gaggtgacga aaatgcagtt
    11341 gaaaagatct ttatctcctt aaaagataaa gaagaattca aagcaggtga tattgaaaaa
    11401 catactaata gcttccgcgt aacaaatcca gatttgctta tctgcagaat ggaaccattt
    11461 gtaaaactgg atatcgaatt gacgattcag aaaggtcgcg gttatttacc tgcagaggaa
    11521 aataaaaatg tagatcgcac tatcggtgtt attccgatcg atgctatcta tacgccaatt
    11581 aaaaacgtgc gttacacgat tgaaaacaca cgtgttgagc aaaaaacgga ctacgaaaaa
    11641 ttattgatcg agatcaaaac tgatggcact atccatcctg aagatgcgat caaagaagct
    11701 tctcgtattc tgatccaaca cctcatgctg atcaccgatg agcatatcac tttcaatact
    11761 gttggcgaaa aagaaacaaa tgttgttgat gagcataccc tgcatatccg caaattgttg
    11821 aagactccac tcgaagacct cgatctttca gtgcgtgcct ataactgcct caaagcagct
    11881 aaaatcaatt cgcttgcaga actcgttcgc tatgacacta atgagctttt gaaattccgc
    11941 aactttggaa agaaatcgct cgtggaaatc gaagccatgg tgctcgataa aggtctccag
    12001 tttggaatgg acctcgctaa atacaatttg gatgaagatt aattcagtgt ttgtcgcact
    12061 gctgtaaaac aaaaagaaaa tgagacacgg aaataaaatc aaccacctcg gtcgtacaac
    12121 cgctcaccgc aagtcgatgc tcagcaatat gggaacatcg ctgattttgc acaagcgcat
    12181 taaaactacg cttgctaaag caaaagaatt gcgtgtatat atcgaaccga tcattaatcg
    12241 tggaaaggaa gacactacac acaatcgtcg tacggcgttt tctgaattgc aaagcaaaga
    12301 agctgttcaa gaactgttta ccaatatttc tggtaaaatt gctgagcgca atggtggata
    12361 tacacgtatt ttgaaaatcg gtggtaaccg cgtaggagat aatgctgaaa catgctacat
    12421 cgaattggtt gattacaacg aaaatatgct gaaaacagct aaagccggtg gtgctaaaaa
    12481 acgcagtcgc cgtggttcag gaaaaacaga gaaaaaagct gttgaacctg aaactaaagc
    12541 tcaagatgct gctgaaggta cttcagaaga gcaagcttaa tttttcagat ataagatata
    12601 gcggggtgat gattatcatt accccgtttt tttatgtacc tgcgataaag agttttttac
    12661 cagccttctg attatttccg gttaactcaa cgatgtacat gccttgaggt gcatccacca
    12721 taattctgct gtctttttga agaatattat cttgcagaat taattgcccg ctaaggttat
    12781 atacattcaa tagataggcg ttttttggat cttcattgag atgtataatg atattatttc
    12841 ctgaagcata catctcaact tctaataatt ccggatttgt tattgcagtt ggttgaagaa
    12901 aagtttcaat agaagtatta gcattatttt cccaggcaaa atctttcact gtaaatgcgc
    12961 gatttgatgg atcggtagtg agtcttatcc agccgtaata tgtgctgtcg ccaactgcca
    13021 attgtaatcc tacaaattta tcttcctgtc caaataccca atcgccaccg gcaaaaatgg
    13081 ggtaatataa atatgacccg atcagtgcat agaattgata agcgagtgtc tgatatggat
    13141 tggtgagaaa ttgacctgca tatggaccaa tattaattcc tgagccaatg gcatatgggt
    13201 aggcataagc gcctccgcta ccggtaaatg ccgcaattgc attatcattt tgtggtgccg
    13261 caaatatgcc attgatgata taccagttca gtcctccaaa ataggtgaac acagctgagt
    13321 ttgcggagga aaaaaagaaa ttaaagtcat tcgttccatc gccatttaaa tcgagagaaa
    13381 ttccggttcc gggttcattt acgagcacat cagggtcaac gtcggtgtag accacatcgg
    13441 ccggagcaac catgggaatt acagctgcag cgagtgttgc atatgctgtg agggtcattc
    13501 cttgttgctt tgacttgtgc atacctataa aacgaaaaaa cctcctgaag gttgcctcag
    13561 gaggttttat ttaacgatct tactgcttat tggttgatca atacgcgttt tgcgtagtta
    13621 gcaccttcgg cagtggtaat tttaacagta tatgttccgg ttgcaaccgc atcgagtgtg
    13681 atttgcatac ctgacatgtt caggccattg ctgtaaacaa cgcgaccttg tatgtcgtat
    13741 acatttacat cggcagaagt tgtgttcaat tcattaacac caatgttgat gatatttcca
    13801 ttagcatata cattcaactg acttggatcg atgttgtgaa ttgcagtagg taattgtcct
    13861 gcttcaatcg cagtttcagg agtgctgagg taagcataat catcaatagt gatttcaagg
    13921 tcatcattat ttacagatac gcgcacccaa ccgtagtatt cattgccatc gccttcaaag
    13981 cgtacgccaa caaattttcc tgtttttccc ggccaagcac cgaaagtata gaattgataa
    14041 ttagatccaa caccgggata atccttaacg ctaaggaaag cacccatata gatagctgaa
    14101 tttgtataga aagcctgacc gccatcaata tccgcgcctg cattcaatgc ttcggcatat
    14161 gctaaagtag cgccgcctgt tgttgaaagc ggataagcaa caacaccatt gcccggagta
    14221 ggataaattt tcattacgtt gaataatccg tcaaagtata ctgagtgata agcaacagta
    14281 tagaagaagt caggtaatgt gaaggtggtg aaattgaaag tgaaatcgat atcgccatta
    14341 ttatccatgt ctaaatcata cacatcaagt ggattcaaag tttcatccgg atccagatct
    14401 gtataaccaa cttgtccttg tgctgatgtg tgcaataaaa ggaaagtacc tgccattgat
    14461 gaataggctg caagattttt acgggataac cctgtagtaa aagatttttt catttgaggt
    14521 aagttttcgg ttagttagca gatgctaata taggtctttt tcaaatttgt agggagtgtt
    14581 acaaaaaaaa agtcccgacg cgatggtcga ggcagtattg ttactgttgg tatccgatta
    14641 attttggata tacagtgatt tgctgaatga agtatttgct ccggtcaatt ggagagtata
    14701 caaacctgta gcagcgttat caaggctgat agccatgttg ctgccggtaa ggcgtgcaga
    14761 gtaaaccaat ttgccatcga tgtcaaaaac attcacattg tcaacaccgg cttcgctgtc
    14821 tttaataaca acgttgatag tgtttccgta gctgtaagca gcaacctgcg cttcagtaag
    14881 gttgttgata gcaacgtctt tttcgcctgc agaaatatca gttccttcag catcatcgta
    14941 agcataatct ttgatggtga aagaaacagg tcctacagaa acatctaaac gtgcccatcc
    15001 gtagtggagt gaaccgtcga tgtcgaattg aacgccgatg tacttatctt ctgtattagc
    15061 gaaaggaccg taagtcacgc cgctatagat agaagcaagc caaagcatgt tgtaagtgtt
    15121 tgatacaaaa gatgcatcag ggccgataag gtcgccatcg ttgagggcag aaccgtaaga
    15181 caggattggt ccggtgtaac cgattaccat gttagaaccg ccaccgtagc cgtaagcgct
    15241 aaggttaccg atcatgtaag cccaagtcca gttaccggct gaattagaga cagcagccat
    15301 caagaaatca gaataaccgt tgttgtcgag gtcgagcgca tacgtctcac ccaactccac
    15361 agtaacatca tcgatgtctg tgtaaactac tgttgcgttg gcttcagagg tagtagctac
    15421 aaaagctccg gccattgctg agtacgctgc taactttcca gcgttttttg aattgtagag
    15481 ttttttcata tctgcagatt atggttaaaa attacagggc gaatgtaagc atttttctgc
    15541 attacacacg ccctgcggac tattttttga caaatttcat ccttaacaag gatgaaggtc
    15601 gtataattat atagacaggt tcaattgttt tttaactgtc aattctgcat taccaaggcg
    15661 aactgcataa ttgcctgaag ccaagtcgtt gcaatccaac acattgtgtc ctgataatcc
    15721 actcatggtt tttacgagct tgccattcat gtcaaataat tgcaaatcag cagttccggt
    15781 gaaatcagat ggaatatcta caacgatatt tcctccgttg ctgaagatct gtgcatttga
    15841 gagagggttt tcttcaatct cggttgcagt ctgaccaacg aaaatggcag tattcggctc
    15901 actttgatat gcatagtcgt ggatggtaat cattgttgca tcatcactta cactaacacg
    15961 catccagcca taatatcggt ctttgtcgat attgattcta aagcccataa aagccatatc
    16021 ggtatccagc cagtttccgc caacataagt gtaataaggg aatcccaaaa cagtaacaga
    16081 agcactcaca gccattgaca ttgcgccatt tgcaaagttg tcgtctgatc ctaccagtgt
    16141 acctgcaggc agaattggtg catatacacc tgaatatccg ctataagaaa caatggatcc
    16201 aaggaattca ttgccattca aagctgaacc caatgcaata ttcacaacat atccgaatga
    16261 gaagccgcta taagtgccgc taccggagaa gttataaatg gagaatgtga agtcgtcggt
    16321 gccatcttca ttgatatcaa tcgaagcttc agcgccttca ccaccgattg ccagatccgg
    16381 gtcgaggtct gtgtaaacaa ctgttgcgcc ggcttgattg atagccagga atgcagttgc
    16441 gctggcagca tactgcaata atttttttgt tgaagtaaaa tcttttttca taagtggttg
    16501 ctatgttcaa atatatgcga aatctgcacc aaaagttaca gtctgcgcct taataaatgt
    16561 taaatggtta gcatcggtca atttatttac ctgatttttc gcggtgaagg cttttatcca
    16621 ctacagcagc catggcatcc acattcatag tgcctccaag gaaagcattg atctttttca
    16681 attcttccgt tgggttattc accagattgc gatgtgatac atataatatc tccatatttt
    16741 gttgctgcgg cacccaggtt tgcacacgct cgagattttg tttgaatgca tttacgatac
    16801 gcaaattcaa tgcatcttct tttgcttttc catcgcgcac caacatgtga tgctggctct
    16861 taatcacttc atcgagatcg cgttccataa atatgatgcg atagcgatag tttgcaggca
    16921 gttcaaacaa taaatgacta attactttca cgccttttcc catggcatct ttcatccatg
    16981 ttttattgcg tgcaagagat ttcaccaatt cgtgttcgta atatccttta ggattatttt
    17041 catcaggtgt gcgaagaccg tcagtaaata tttctaatcc acctttatcc agcatttgca
    17101 tcatcatact tgttcctgaa cgcgggagtc ctgaaacaac aacgatatca cctttgatat
    17161 aatctttaat ctgtgattta acttcaacag cggcttcagg ttttttaaga tgctcgctat
    17221 aaatagttga taataattga cgcgctttat taattcccgg cgccattttc aaacacactt
    17281 caaatgcttc ggcactgcgt tcgtattcgc cgagataata taatgcttca cccaagtgat
    17341 agtgtgcaaa tgggaagaaa tatgtcaatc ctactgcatt caaaaaacaa tcgatagact
    17401 cttcgtaatg ttttctgcgc aagagagtta tacctaaacc atgatgtgca gatgcatttc
    17461 ccggatcgtg ctcaagttct ttgaggaaag cgccttcagc ctttttccaa cgctttaatg
    17521 ccatgtagca tctgcctaat tgcatgtgca cgcgcgccgc atttggcatt tctttttcaa
    17581 tggcttcgaa taattccatc gccattttag gtttattctc aacgagcaat aaattgcctt
    17641 ccatgatttt catgctcacg gattcgcgac cattattttc agtcttgatt ttagaaagca
    17701 cttcgcgcgt ttcttccact ttattcatcg actgataagc atgagccaat cgcatagcat
    17761 agcgattggt gccgggattt tcgtcaacca gcttcttcag aatgtccact gcctcagcat
    17821 attttcttcc gttcatataa gaacgtgcga ggtagtaatt attttcacta acagtgctct
    17881 tgattgcttt ttcaatattt tcacttggcc tttcaatata tccaagttca atgagttgct
    17941 ccatcgcttc agcagcagca tatgggtctt cttgtttatc ggccgggtgt tgacctgaat
    18001 caccggggat atcttcccag gtatcaatct tgtccggaat aacaggtgtt tccaatgcat
    18061 taacaagcac gtttccatcc atatcggcag caaccggcaa tccgaatagc gtaagcaacg
    18121 taggtgtaac atctaatact gatgcgccat aaatacgttc atctttttta atgccgggac
    18181 cgttgagcac gaaaatgcca taagggctat gttccaatgc aggagctgca ggttcttttg
    18241 gtaattctat tggacgaaga tggtcaggat ggaaaccgtg atctgaaata atcataacag
    18301 taacatcagg acctgcaagt tcgagcaggc gctccaacat catatcatga tatttgtaac
    18361 caccggcaac gacatcttta tataaattat ataaatcgtc cggtaccatt ggcatttgtg
    18421 gcggatggaa tttcatgaaa ccatgacaga agtgatcgat ggcatcgtaa tacactgcaa
    18481 ggaaatccca ttcttcattt tcaagaatat aagttgcagc attatgaatg gttgagcaat
    18541 cggcaataat ctttgtgagc atgcccaatc gcttatcttt ttcctgatca attttattcg
    18601 catccggaat aaaaggtaaa atgtgcgatg ctgttaattc agcagggtgg atacgcattt
    18661 ttttgaacag ctcagcttta tctgcaggat gtacagttcc atcgagcata ggccatggtt
    18721 cgttgattgg ctttgttgct ctttgataaa gattgctgat catagtgccg ttgattggct
    18781 cagcaggatg tgaaggccac catccgatga cgtgactttt cagattatgc tgattgaaga
    18841 tattccaaac agcttttact tttctggatg taatatatac aggacggata ccttttcctg
    18901 atggatccgg ttcggtaaaa ccaatgattc cgtgtttata aggacgttta ccggtagcga
    18961 tagaagtcca taatgttggc gacagtggtg gatcgagtgt tgcatgatta ccaatcaccc
    19021 caccgttaac taacttttct aaagttggca ttaaacctgc gtccatcagt ggattgatca
    19081 ccttccagtc ggcagcatcc catccgatca gcagtacttt ttttgcaata cgtttaccca
    19141 tgtttaattc taaagtctaa taaattggag ataaaaaaat gagccaatgc gtcttttaat
    19201 ctgctattgg ttcatcttgt tgttgttcgg tgtttccacc ttgtttggct tctttcattt
    19261 tctgaaattg ccatttagcc tgacgccagg cgatgaggcc tttatatccg agcgccagca
    19321 atccgagaga tccttccacc gggatgcgaa acggtttgcc gtcttgcgtg gtgatacgga
    19381 gcttattgcc ttgttttttt tccatacacg attaaattgt tatcctgacc caatgcaggc
    19441 aggacgcaaa tttaacctaa accaagcggt aatgctaata tcagaatgat gattttactc
    19501 gcttcaggag gagtgcagtg catgtgaaga tcagcggatc aaggtaatat tcccttgcat
    19561 atgctgcttc atgccgtctt ccaactgcac tatcagcata aaagcataaa caccttgtgc
    19621 aacaggttct tcattataag tgccatccca acctgccgat gggttgccgg cgatcatatt
    19681 ttctttggtg aatatcaatt ctccccaacg atcataaata ttcatagata ctatgctggc
    19741 gaggtcggtg ccatatgcga agaaatagtc gttgatgtta tcaccatttg gcgtaaatgt
    19801 attcggaaca aaaacaacag gatcgaatac gggaactact gtaatagtaa ccatatcact
    19861 gtcgatgcaa ccaaaggcgt cccaactggt aaccatatag gtttgactga gcagggagtc
    19921 aaaaacagga gcagggcaat cggtgcaact caatccggca ggaggttccc aaaggaatgt
    19981 agttccacct gtagcagtga tcacggtagt tgtatattca ggaatatatt gatctattcc
    20041 tgcgtcaaca tcattttgta tgacgttgat cgcgccggta gtattgtttg tacagccatt
    20101 ggcatcggta taggtatagg ttacggtgta taaaccgagg tcacctgcca atgccggatc
    20161 aaactcgtcg ccgataacac cttcgccact aaatgttccg cccgcaggat caccaataat
    20221 cagggtaggg tcgccttcga gacaaatcac gtcaatgaga tctatagtag gtgttggcag
    20281 cgggtaaata gtaacttcta cggtgtccac ttccgggcag ccggggctgg tataataaat
    20341 ttcataaggg cctccggggg tgcatgcagc caggttgagt tcaccggtag cagcgttgat
    20401 ggagataccc gccattgcgc tgaaagtgcc tccgggatta agaatcatgt taggcagggt
    20461 ggttccggtt gcacaatatg caggcagtaa gtaactaaaa gctgcatcgg tcaatggcat
    20521 gatctcaatc actacgcttg tgctgttgga acatggtcct gccggggtgg tatacgttat
    20581 ggtgtaaaag gaaccgggta tacctgttgt aaggtcgata gcacctgtaa gtgcattaat
    20641 agataaagtg gccggactga cagtaaaatt accaccggga gtttcaacgc tggtgacaat
    20701 tgccgttcca taattgcaat aggaagcagc atcatatgta aaaccagggt cgtccaatgg
    20761 atcgatggta actgtttcgg tgctgctgtt gctgcaaggc cctgcaggtg ttgtataggt
    20821 aattgtatac gttgtgccga tgtcaccact tgccaaatta agcatgccgg ttgttgaatt
    20881 gattcccatg gttgtcggga atatgctaaa tgttccaccc ggggttgtta tagaagtcgg
    20941 caatgtggtg cctgtcgggc aataggaagg agcgctataa ctaaaagttg ggtcatcgag
    21001 cgggtcgatg gttattgtcg tagttgtgct attggaacaa ggacctgtgg gggttgtata
    21061 tgtaatagta taggttgtgc caacagttcc ggtagtcaga tcaacaactc cggtggtagg
    21121 atttacaacc agaccggcgg ggctaacggt gtatgtaccg ccgggagtag caacagttgg
    21181 agttgcagtt cccgtcgggc aataacttgg ggcagggtag ttcacggaag ggtcatcaaa
    21241 atcagtaatg gtaatggtga cactgtaagt gcttacacaa aggataccaa ctgtgtatgt
    21301 aatagtgtat gttgtgccta tggtgccggt tgtgaggtta actacaccgg tggttgggtt
    21361 aacagtaagg cctgcaggag acactgaaaa tgtaccgcct gctgtggtaa taaatgtagg
    21421 tgtagtggta ccggtggggc aataattgat atcgctatag ccaaacgatg catccgggtc
    21481 aacaacaatg gttacgaggt ctacagccgt ttcagaaggg caatcgcctg taatgccata
    21541 agcggtataa gaaacgctat aactacctgg tgttgaggcg tttaggtcaa tttcaccggt
    21601 tacgggatct attaccattc cggcaggaac ggcggcaaaa gttccgcccg gatcgccggt
    21661 aacagttggc atggcatagc cggtagtgca ataaaatgga tcgtaagaga atgaaggatc
    21721 cggatcgtcg acagttacta caatatctgc ggtataatcg gttccggaac aggattccag
    21781 aaatgcagtg tatgtggtag taaccgtagg gctaacgatg agagaatcgc catagccaac
    21841 gatagctcct gccggatcat accagataat gccgttaggc acaaaacgag tagactcgtt
    21901 agtggtagtc catggtgttg aatttcttcc cggcgctgtg aaggcagtaa gaccatcgcc
    21961 gctgtgaaca ccttgtgttg cggttccacc ggcccatgcc atgcaatttg gcttgttagt
    22021 gaggtggttc tcaataacat ttgtggtttc gtttaaaaca atttggaatt tcccgtagag
    22081 acctgtgcag ctaaacatgg gtacgttatc ccaacttaca acaaatttcc tgtttggagc
    22141 agtgcctatg gtttggtatt tgatacaact gccaacaggg gtgcataatc cgggatgcca
    22201 gtcttgccat ggtcccatga tgcaattttt tggcactgtt ccggcaacgc tgggaatagc
    22261 tgctgaggta aatgtggtaa ccaaagcccc cggacctcca aaactcaccc agccattaga
    22321 gcctatataa cattgtgtat attggtttcc gaggaagcag aaagtaaagc ccagaggaac
    22381 agcagtagag taactgtcat ctgttaatcc gccaccaccg gcttggattg gagaagtgcc
    22441 ggcatatgtt tccggactat agggataaac ttcgaaggta tagctgcttg tgccgtagga
    22501 tggagaagaa ataactgcaa gggttgcggt gccgcctgca caaatagttg tatccggcgt
    22561 tacaacaagc tgtgcgggta atatgcttga taagcaaatt accgataaaa aggccgtcaa
    22621 tttcctcatg tgctatgcga gtccctaaaa gaggggaaag ttactacttt ttcaggattg
    22681 caaaaaaagt tggggggggc tcaagtaata caaaatgata ggtactcgaa aaaaacaaaa
    22741 ccccggcaga taaactaccg gggttgctcc ccaacctggg ctcgaaccag ggacctactg
    22801 attaacagtc agtcgctcta accagctgag ctattgagga atgtttcact ggaaacggga
    22861 tgcaaattta caatcttccg tgtgcattgc aagtatgccg cggaattttt ttctgaaagc
    22921 tgatgaaacg aagggattgc cgttactttg cagcaaaaaa taagacatga gtttattagt
    22981 agttggaaca gttgcatttg atgctataga gacaccattt gggaaacgtg ataaagtaat
    23041 aggcggttcg gccacctata tcggattatc ggcgagctat tattataaac ctatacatat
    23101 tgtttcggta gttggtagcg acttcccgaa ggcaacactt gcagcattca acaaaaaggg
    23161 tattaccaca gatggtgtgc aggtaataaa ggggcagaaa tcttttttct ggtcgggtaa
    23221 atatcatatg gatatgaatt cacgtgatac tttgcgtacg gatttaaatg tgctggcaga
    23281 tttcaatccg gtgttgccag aaaaattgcg caagcccaac atgttaatgc ttggaaacat
    23341 agatcctaat ttacagattc gtgtgataga gcaattaaaa tcgcgcccga aattaattgc
    23401 gatggatact atgaatttct ggatggatat tgcgcgtcct gctttagata aagtgcttaa
    23461 gatggtggat gtgttaatta ttaatgacga agaggctcgt cagttaacag gtgaatatag
    23521 tgttgtaaag gctgcaaaaa aaatcatgaa gaacggtcct aaaacactga ttataaaaaa
    23581 aggtgaacac ggtgctttat tattttccgg ttcagaagtg ttttttgctc ctgcgcttcc
    23641 attggaagaa gtatttgacc caacaggtgc cggtgatacg tttgcaggtg gatttatggg
    23701 ttatttatca cagagcaaaa ccttgtcgtt taccaatatg aaaggcgcaa ttgtttatgg
    23761 cagcgcaatg gcatcgtttt gcgttgagga gtttggtcct gaaaaattat tggctctcaa
    23821 aaagcctgag attgcgaagc gtgtgaagat gttttcaacg ttatccaaat tttctatcaa
    23881 gggataaggc gaccaataat attgaatttt tgcagtgtga tttctgtgtg tggggcgtct
    23941 tttaactcgt cggtaaggtc ttgtccggca aagtgttcgt agtgtttgcc atttcgccat
    24001 aaacgcgaag gggtgacatc gtaaatcaag cccttatagg caatccagat ttcttcttta
    24061 tcttgaccat tgcgcaaggc gagttgaaat ttattatagg cgggcaatgt gtttacatcc
    24121 ataaatgaaa ttggttaatg gatgaaattt gagtaaagcc atcctgagca aacaaatgtt
    24181 gcgctgcgat attatttcct tgcgtatata aattcatgac cggcaattga tatgcatttg
    24241 caaaatgatc tgaggcggtc aataattttt tggcaatccc ctgatttcgc ataccgggag
    24301 caacggctaa taacccgatt tgcatatggc tttttttatt tgtgaaggct atcagcccca
    24361 aggggttaat ttctgttcca ataaaataaa tttcatcgcc cattttccca tgaagtgatt
    24421 cacgtatcca catggcatac agagcagttg tttttttctt attgaaatga tcatcacaca
    24481 taaatctgga gtgctttccg gcatgtattc caagttcaat aatgccatct gtcacttgtg
    24541 ttaaaatttt cacttcggga tttaattgag gtgcacgggt ttcgcgttga agcagacatc
    24601 gcgagctcat aggatttaat gtagcggatg acctgttgct aatttcgacc tccgttgcgc
    24661 tatcaatata caaacaagtg tagccttctg aacgccagtt ccctacttga tgttcggtaa
    24721 ttatgcaggt gctttgtaat tcaaggcgac cgattttaaa accgaagaac cggctgtccc
    24781 agtcgaggta ttcgatttta atatcgggtt ccatattaca tgactaattt tgagtgaata
    24841 ataagactta gctttacaat gtcaattcca atatgctaac catttctaac ctcaccaagt
    24901 cgttcggcga tcatgttgct gttgatggca tttcgctatc gatagccaag ggaatgtttt
    24961 atggattact tggcccgaat ggtgccggta aaactactac aattcagatg atcagcagca
    25021 tattgcctcc cgattccggt aaaatttcag ttgatggatt aggcgtttat gcgcacgcat
    25081 ccgtaaaaat gcacatgggt attgtgccac aggagattgc cttatacgat gaattaaccg
    25141 cattagagaa tttgattttt tggggtaatc tatatgcagt tcatgggaag accggaaaag
    25201 atcgtgcagc atttttgctt gattgggtag ggttggcgga ccgcaggcat gatattgtaa
    25261 aaaattattc cggaggaatg aagcgtcgtg tgaatattgc tgccgcactc atgcatgatc
    25321 ccggtttaat tgtcatggat gaaccaacag ttggtataga tcctcaaagc aggaataaaa
    25381 tttatgagct gcttgatgaa ttacatcatc agggaaaaac aattttatat accacacact
    25441 atatggaaga agctgagcgt atgtgtgata aaattggcat tattgataaa ggtaaaatca
    25501 ttgctgaagg atcacttgat gaattgaaaa aagcacacgc gcaggaggag aatattgtaa
    25561 ttcattttac cggtgaagcg gtaagcaagc ttggtaatta tgttatttct tttccggagc
    25621 cgcaaatgat gactgtagtt acgccacagg ctcgaaagcg tttacatgat gttgtgcagt
    25681 tgtgtactga agggggaatc gacatcacag gaatagatat tcacgatgcc ggattggaaa
    25741 gtatttttct taacctaacc ggacgccagt tgcgggatta atataattcg tatgtggact
    25801 attgctaaaa aagatattta tattttcttt aaggataagc gtggattgtt tttgtcttta
    25861 ttgttgccaa taggtttaat caccatgttc gcgtttgcat ttggcggaat agggcaagat
    25921 gatgaagatc cggcgccaat acatgtatta tatgttgatg cagatcaaac gacttcaagt
    25981 aaatcgctta ttgccatgtt ggatactatt ccgggaattg cttgtttgtc aactacggcg
    26041 gaagacgcta ctgctaatat taaaagtggc gatgaaatgg cggcggtcgt aatcaacaaa
    26101 ggatttgaaa tggctttgca aacgggtgaa gatttgccgg ttgttttgca atacgatcaa
    26161 agcagagtaa tggagattgg tgtgttgcaa caattactta caagcagatt gtcggcaatg
    26221 aaggggtcgg tagatgcaca tagaggcatt aataaaatta tgcagcaatc gtttagtaat
    26281 atgccgccaa atgtgcagga ttcaattcga acatcacttg aagaaaattt attgcagaaa
    26341 gaaaatcatg aacaattaat aagcatgcag gaagtagtgg gtgatgccga aagtaattgg
    26401 gggttaattc aggcggtggc aggcacggca attatgatgt tattatttag tgtgagcagt
    26461 attggcaaga gtatgttgga tgaaaaggaa agtggtgtgt tgcgcagatt gcttcaaact
    26521 cccatgcatc catatggatt attatttggc aaaatgataa ctgctttggt tattgcggta
    26581 tttcaattaa ccatcatgtt tttattttca tggctggcat ttggattaga tatattcatc
    26641 aatataccgg cactaataat tatgattttg gcaacctcgc tggcatgttc tgcttttggt
    26701 gtattgctgg caagtttggt taccacccga aaacaggcgg attcgttaag tactattctg
    26761 atattattta tgagtgctgt tggcggtagc atgattcccc ttttcattat gccggttttt
    26821 atgcaaaatg ctgcagttgt ttctgttaac tattggagca ttcaggggtt ttacgatatc
    26881 ttttggcgcc aggcgggttt ggaagctata gttggcaatg cgctggtttt aacaggtatt
    26941 tccgcagggg ttttgctaat aagtatgtat ttttataaaa aaaatattct caggcttatc
    27001 tgaggtcaat tttattagtc agacattcat gaaaagaatt ctcctctcct gcataatttt
    27061 tagcggattt attgcaagtg ctcaggatgc attggatcca tcatttggtg gtgatggtat
    27121 tgtaacactt gattttggcg gtaatgatat tgcatatgca gtattgattc agcctgatgg
    27181 taaaatactg gctgcaggtc aggcgggaat tggcccggca ttaacaactg atattgccat
    27241 cgcccgatac aacaccgacg gaacccttga tccggtctgg ggtggagatg gcactgtaac
    27301 gctcgatttg ggtgtagatt acagcgactt ttcatttgct ctggcaagac aacctgacag
    27361 taagatagtg gtagccgggt gtggcttttt cgattctggt tataagtttg ctgtgttacg
    27421 ttatagagca gatggcagtc ttgatccggc tttctctgat gatggcattt ttatcactga
    27481 ttttggaggt atggacgatt gggccagagc agtgtacgtt atgcccgatg gaaaaattgt
    27541 cgcctccggt tatcgcaatg atggtttgaa acataatttc gccattatac gtcttacgcc
    27601 ggaaggagac cttgatccta cttttggaac cggtgggaaa gtgtatactg tttttcccgt
    27661 aaatcgccac gatcaaagct atgccttagc tgttcaacct gacggcaaaa tgattctcgc
    27721 cggttatagt tatgttggca caacagcaga ttatcaattt gcgttagccc gttataatga
    27781 agatggttct ttagatgata ctttcggtga tgcgggccta gtgttaacac ctgtccctat
    27841 gatacagggc gaaagctatg ccatggaatt acaacctgat ggtaaaatac ttgtatccgg
    27901 attctcatac accgattgga aaacagatat tgccatcata agatttaata gcgatgggtc
    27961 agttgacact gaatttggta ccgatggatt ggtgaagaca gatattgcaa ctgatcgcga
    28021 tgatcatgca tacgccatgt acctgctagc cgatggaaga tttcttgttg gcggaaacag
    28081 atggaatggt tatgattggg atatggcatt attgcgatat aatgccgacg gatcacttga
    28141 taatactttt agcgggaatg gcatgcttac aattgccatt ggcgatgacg cggactgtat
    28201 tcaggctatt gatgtacaaa gcgatgggaa aattgtagtt gcaggatata gcgataacgg
    28261 gcctaacaat gattttgcac ttgtgcgttt tatgcaagac ggcggttcag gagttggaat
    28321 cagcaatcag gtcgtaacag caaatttcag tatctcaccg aatccgggat atgataatat
    28381 ttctatcaat tgtacattaa gtggtattat tgatattgtt gatatgcaag ggcatgttgt
    28441 tgaacgtcga gaagtggtgt cgggagataa caccatcgac atacatcaat taccgacagc
    28501 aaattatatc ataagatggc gaaatgaaag tgcgcagtgc agtttgcctt tcatcaaaaa
    28561 ataatattaa tattattctt cgttatcagc gttcgttttt ttcacatcat atttaggtaa
    28621 ggcaaacagg aagccgaatg cctctccgtc tttacgtccc aattttttat gatgaatttt
    28681 gtgggcacgg attatacgtt gcatatattt actttcagga cgcatatggt gtttcatacg
    28741 acgatgaaca agcacatcgt gaaagccaaa gtagcataat ccgtatgcgg aaatcccggt
    28801 tcccacccaa aaaatccaat tataagccgg cgacccgata tacatcaaat agattgcagg
    28861 tgctgcaaaa atgatggcgt acaggtcatt caattcaaac ctgccgtggc gaggttcgtg
    28921 atgggagcga tgcaagaacc agcctacacc atgcatgata tacttatgca taaaccacgc
    28981 aaaagcctcc atgaaagcaa aggctgcaag aaaggcaata atattaatga tgatgtccat
    29041 cggtagttat aacaaagtta ttggtcttgt gttcatcatg caccaatcag ggcattcttt
    29101 tttttcgaac gtgatttgaa tccttcgatc agcataatat tcatgagcat aaggctgaaa
    29161 ctgtacaaag aatcttctat ggggatggtg cccattctga ttccaaggtt ttcatgatta
    29221 ttataccaaa ctacttcttc ggcaattccc cacccggtta aaattccatt cacaataaaa
    29281 aaggggatca ggtgccaaag gtaaaatcga taaaatttcc ccatgtatgt gccttttacc
    29341 aaaaacaact gaatgcatag caatacaaca gcatatccgc aattcaacgc agtatacgct
    29401 ttattgtaat ttgcaaccaa gaggattaaa gcgatagtca gcattgcata gcttatcttg
    29461 tttgcatatt tgtctaaagt tggtgtgtcg ggccaaattg tatttgtaaa agcgtaaatg
    29521 aaaatacttg cataaggaac agtgaagaaa aataatattt cttctatcgg taaattcatg
    29581 aaatgggcgc cggtaatata gcggtcatta aatccccaaa ctccattgac tgtaaatatt
    29641 tgatcccagg ttaagaatac aagagccgtg atgaacatag acggaaacag ccatttccac
    29701 caattgacgt aacgcactct ggattcaaaa ctgaagagaa atggaaacag gatggtgcct
    29761 gtattgatca gcagatacag gtaactctgg ttcatggtgc atagatacct aaaacatttg
    29821 aacgcaggta aagagatgcc cacaatccga ttttttttgg atttgacact cttattcttg
    29881 tttgcataac agcttcgggt tctgtgcttc tgattttttt gaaaagctcg agatagtatt
    29941 tatacgccag ataaacaccg aattttgcac cttccggcaa ttgctttatt cctttgtagg
    30001 cttcatcaaa gtcatttgcg atttcttgct caatttgtgt tttctgacgt tcacagaaat
    30061 tttcgaaatc aacatttgga aaatatacac gtcctctatc cttataatca gattgaagat
    30121 ctcttaagaa gttcaccttc tggaatgcag caccaagaaa tctggcaggt tgaattagtt
    30181 tttgataatg cgtttcatcg ttttcacaaa atacttttaa acacattaga cccacaactt
    30241 cagcactgcc ataaatgtat tcttcataat gatgttgttt ataattcgac atatgaagat
    30301 ccatttccat gctgtgcaag aatgcatcta tcaaatttct gtcaatttga tatttatgta
    30361 cagtaagttg aaaagcgtgt aatatcgggt tcaatgaaat tttttcatcg agtgcactcc
    30421 aggtgtcttc cttaaatctt agtaaaaggg tttgtttatc ccaatcgtga aaggtatcaa
    30481 caatttcatc ggcgtagcga acaaatccat agatagcata aatagcgtca tggtatcggc
    30541 ggtcgagggc tttaataccc aatgtaaagg aagtgctata cagctcagtt accttgcgac
    30601 tgcaggtgaa tgacgattcg tggaatagat gtaacatacg cctgattagg acattaaaac
    30661 taattaaata cggaattgtt cactgaccgg atcaatgtcc aacaagtttt tccatctcct
    30721 gagcagctac ataccctgat atgagtgatg gtggcacacc ggggccggga acagtcagct
    30781 gtcccgcgta gcaaagattc cttacccgct tgttacgcat ttttggctta aagatggcgg
    30841 tttgcatgag ggtattggcc aatccgtagg cgttcccttt acaagcatta tatctgtcga
    30901 caaagtttcc gggagcaaac tccctcttat aaaccactct tgatttgatt tcttcaccgc
    30961 aatgtgcttc aagccggtcc attgctttat gatagtattc ctctcttagt tctgcagtat
    31021 ccttgagtcc ggcagcaaca ggaaccaaaa tgaagagatt ttcatctccg gcaggcgcta
    31081 cagtcgggtc agttactgat ggtgccgaga cataaaatag cggttgttca ggccaccttg
    31141 gatcggtata tatttcatca gcaaattgtt caaaactctg atcgaaaaat aaattgtgat
    31201 gcagcaaatt tttaactctt ccctttacac ctaagtaata tataatagca gaaggtgcaa
    31261 agatcttttt atcccaatat gtttcatcat aattgcgata cggctggtcc agcaatttct
    31321 gttcaacgtg atgataatct gcagctgcta ttattcctga cgcggcaata aaatcaccat
    31381 ggactttgac gcctgttgat ctgccttcct ctacaataat tttttcaacc gggctattaa
    31441 actgaaattg tacacctagt ccttcagcta cacgctgcat gccttcaatt attttataca
    31501 taccgcccat tggataccag gtgcctaaaa caagatcggc ataatttaat atcgaataaa
    31561 gtgcgggtgt atttgatggc aatgctccaa gaaagtaaac agggaattca agaatttttc
    31621 tgatgcgttc atttttaaat ttggcatata cctcactgcg cagagatgtg aataattgca
    31681 tcgtaaataa tgcctttacc atcctcagct cggcaaactc cataatacta ttgcagggcc
    31741 gaaaaacgag atctttcatg cctacttcat atttgtaggc agcgtcgtcg agaaatttct
    31801 gtaattgaat tcctgacccc ggttctattg attcgaatag tgcctttaat tccgaagtgg
    31861 atgccggaat ttccatcaca tcgccgggtc cgaaaacaat acgatagcca ggatctaatc
    31921 gcttgagtgc atattgctgc tcaacggagg atccgtaagt attgaaaaac cattcaaaca
    31981 catcgggcat ccaataccaa cttggtccca tatcgaaggt gaagccttgt tcgctaaaaa
    32041 cactgcatct gcctcctgcc gattcatttt tttctaaaat ggtaactgaa tatcccttcg
    32101 ctgccaaggc acatgcagcg gaaatcccac tgaatcctgc gcctatcact attatatgct
    32161 ttctttcggc cataattgat ataagcgaat gaaacagcta attcggtgaa atgtttagca
    32221 ggagcgtttc aaataatctc catcagggtg cgaaaggcga agaaatcatt gccaaacttt
    32281 tcctgagtat cgcgctgtaa tgagggccca gatgtaagtt tataggtcag gaaatcatcc
    32341 aaattgttga gtgtgtattg cacaacaaag gtcattcctt ccatatcacc gtcgtcaacc
    32401 agacggcaaa gtcgatgttc cacaaagaaa ccggtagcca ttacatcagg gatatgtttc
    32461 tcgcgcatcc aggtaagcca ctcgtcggct ctggattgtt caatttttat ggtgacgttg
    32521 tatagataca tgtggtaaaa ataagaggaa aaaaggatgg ttggttagca agtttgaggg
    32581 ttagcaggtt agcaagtggt ggggaattag caaatttggg aattagcaga ttagctaatt
    32641 gggaatgtgc ttgcatgaag acgctcgtca aagtgagcaa gtttgagagt tagcaaatta
    32701 gcaagtgtga tgggctttta acgctggcgc gttaataggg aatgcaattt attgctttgc
    32761 aatattttaa ttccgaagcg caatcagctc cctctctatc gacg
//
LOCUS       DAOQOU010000015        32559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_604_length_32559_cov_6.180070, whole genome shotgun sequence.
ACCESSION   DAOQOU010000015 DAOQOU010000000
VERSION     DAOQOU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 32559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32559
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_604_length_32559_cov_6.180070"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PLM90_02480"
     CDS             complement(<1..1276)
                     /locus_tag="PLM90_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88262.1"
                     /translation="MKPIILTISLFCYILTSKAQEALYCDGKLLAYQLEAMGAENGNY
                     YLLCATGSDSLYLIKYGPDHSILWYQKFAPNFGVQIALHKDKAHLLIQYADSSTLYIF
                     NSNGDIFAKRTYTDEYKSLFVKYDRIILNTVGGPYDNYPHPKILILDENTNIIGTYED
                     TEYNTEFTMVQVDHLQNIYLGHNVYAWEDDGIGHTLYNYKYTGLIKLSPDGNKLWLYD
                     DLAKTVSNENSAIDFIHVDAQNDIYLYLQNYKLQLNELGVLERNSELLTGHFLAQPDY
                     QYVPCKGVQLAFFDLVEDAFFTCRFNDDDPFELDFVNTYSLGTTTTSCTLPEHYLDPN
                     GYITFSLRIFSPDQCSLQCFDIDGNENFFDIDPTWGPHPYSQSEDMLPQPDGNILYFY
                     NTFEQIDDEELLILYRTERNIAYPTADLSPECP"
     gene            1559..3172
                     /locus_tag="PLM90_02485"
     CDS             1559..3172
                     /locus_tag="PLM90_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88263.1"
                     /translation="MKIILNCFFLICITSVGYSQTFEKVYEKRYFFACESLDNSYLLV
                     GEDPVDDTLDNISLLRISAINGNVQSEYIYSLEGFDLFPNFVIETDDGGAYICAILRY
                     DNLHGDPIIIKIDACGNPLWMRNLNEIFCPYLGLSYLNEVNYATLDGNGGLTFVIGLE
                     CVSFYCFNIDQYGIVRWVSNAIGEESWMMSGTNFRRMDDGKYVLTGYQHTYWSDTVLY
                     SSEYKINAAIRILDSIGNFVDEEEYMLDSFESMAYGICDSWVSGQYVGTCHTGVEHYT
                     ILAVSNELDSISHYEVASMYDYVLLPLDIYQGPKGQYYIIGNSCDTIPLLHSGLGAQL
                     IDSYGNVLAQNSTGFDINYGYRNSFVTSDLNVVILADVRDDSETAESEFDLDTYLISF
                     DQDLNISLITDTVTPVSAISEACGYETATVHFDLSPIMEEGLAYTLPDSSLIIHEEAD
                     KANAKNWGAINVYPNPMTDKALITWEKEDPVVGIHVYYPNGSLLTSVEIGEAIQYWQF
                     DRGNLASGLYAYIIIRTNGSQSAGLIVLQ"
     gene            complement(3260..4648)
                     /gene="accC"
                     /locus_tag="PLM90_02490"
     CDS             complement(3260..4648)
                     /gene="accC"
                     /locus_tag="PLM90_02490"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ88264.1"
                     /translation="MTKKSSKPQELIRPIKKILIANRGEIALRIIRTAKEMGIKTVAV
                     YSTADKDSLPVRFADEAVCIGPPSSKESYLNIPKIMAAAEITNADAIHPGYGFLSENE
                     MFSAICAKYGIKFIGPMPEQIHQMGDKITAKETMKKAGVPVIPGSEGLLKDIADAKKQ
                     AKRIGYPVIMKATAGGGGKGMRIVWNESEVENAFNTASMEAKASFSNDGLYMEKYIEE
                     PRHIEIQVAGDRYGKACHLSERDCSIQRRHQKLVEESPSPFMDDKLRQKMGDAAVLAA
                     ESIAYEGVGTIEFLTDKHRNFYFMEMNTRIQVEHPVTEEVVDFDLIKEQIKIAMGEKI
                     SGKNYFPELHAMECRINAEDPSRNFAPSPGRITSFHSPKGHGVRVDTHVYAGYTIPQY
                     YDSMIAKLICKARTRQEVILKMQRALDEFIVEGIKTTIPFHKKLMRDERFIAGKFDTS
                     FMNSFDLSNLGD"
     gene            complement(4663..5157)
                     /gene="accB"
                     /locus_tag="PLM90_02495"
     CDS             complement(4663..5157)
                     /gene="accB"
                     /locus_tag="PLM90_02495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HMZ88265.1"
                     /translation="MDFKEIQELIRMVNKHNVAELTIENQDFRIKIKTALAATTMVQA
                     PMMMPQMQQPVMMPQMSAAPEKPQMAAAETKSDAAESTGGNTITIKSPMVGTFYRSPG
                     PDKEAFIKVGDEISVGSKICIIEAMKLFNEIESEVSGRLVKILADDASPVEYDQPLFL
                     IEPK"
     gene            complement(5163..5726)
                     /gene="efp"
                     /locus_tag="PLM90_02500"
     CDS             complement(5163..5726)
                     /gene="efp"
                     /locus_tag="PLM90_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HMZ88266.1"
                     /translation="MASTADIRKGLCIDFNHDIWQIIDFQHVKPGKGNAFVRTKLRSL
                     TTARVLENTFPSGATINPVRVERRTYQYLYNDETGYHFMNNETFDQVTLDGEMVDGKE
                     FFKEGQEIDILFHADTEKPLFCELPAHIILAVTFAEPGVKGNTATNAMKYATVETGAR
                     IQVPLFVEEGDVIKIDTRTFEYVERVK"
     gene            complement(5835..6830)
                     /locus_tag="PLM90_02505"
     CDS             complement(5835..6830)
                     /locus_tag="PLM90_02505"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMZ88267.1"
                     /translation="MQKTLAAITGVYGYVPEYRLTNAELERMVDTTDEWILTRTGISE
                     RRILKGEGLATSDMVVEAVKGLLAKTGNTAGDIEMLIVATTTPDMQFPATANIACDKL
                     GCNHILSYDISAACSGFLFALSTAAQFIESGKYKKVVVVGADKMSSIINYTDRATCVI
                     FGDGAGAVMLEPSTDGNGIADAVLKSDGSGRHFLHQKAGGSLKPASHETVDNMEHTVY
                     QEGKTVFKFAVKNMADAAEEIMLRNNLKSDDISWLVPHQANLRIIDATAERMGLSKEK
                     VMINIQKYGNTTAGTIPLCLWEWEPQLKKGDNIVLAAFGGGFTWGATLVKWAYNS"
     gene            complement(6869..7048)
                     /gene="rpmF"
                     /locus_tag="PLM90_02510"
     CDS             complement(6869..7048)
                     /gene="rpmF"
                     /locus_tag="PLM90_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMZ88268.1"
                     /translation="MPNPKRRHSKTRTRSRRTHHGVDMPNVSACANCGAPVLPHRVCP
                     ECGSYRGKIAIEKKA"
     gene            complement(7060..7563)
                     /locus_tag="PLM90_02515"
     CDS             complement(7060..7563)
                     /locus_tag="PLM90_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HMZ88269.1"
                     /translation="MKQGVHHFNYELDDVFFGHFPESQVQQGKVFVDLALDKKERLFV
                     LNFDVSGSISTDCDRCGQPFDLPIHGNYTIYVKLGDKREEDVDSEEVVWIPDSESILD
                     ISEMVYEFVHLSVPMLKAHPDKAGGVPGCDPEILKLLGQEEEGSQDEKGPDPDPRWDI
                     LNNLNIN"
     gene            complement(7732..8790)
                     /gene="pdxA"
                     /locus_tag="PLM90_02520"
     CDS             complement(7732..8790)
                     /gene="pdxA"
                     /locus_tag="PLM90_02520"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HMZ88270.1"
                     /translation="MENRIRIGFTLGDVNGIGPEVVIKALEDKRMLGLCTPVIYGSNR
                     IISFYKKLFNLEGLSYTTINSADQANAKAVNIINCITQEVMIMPGQENETGARVALTS
                     LEAATNDLLKGQLDAIVTAPINKHNLQSIGFNYPGHTEYFNAKAPESESVMLLMQGDL
                     RVGLATGHIPVKDVASALTKEGLLRKLEIIHHSLKQDFASTGPRIAVLGLNPHAGDSG
                     TLGAEESNIIIPAIKAAQEKKMFVTGPFSADGFFGSGQYQHFDAILAMYHDQGLVGFK
                     SLSFGGGVNFTAGLPFVRTSPDHGTAFDIAGKNEASAESMLQAIFTAVDIVRSRKVYH
                     EITANPLKRTEVASERGA"
     gene            8893..9666
                     /gene="rsmA"
                     /locus_tag="PLM90_02525"
     CDS             8893..9666
                     /gene="rsmA"
                     /locus_tag="PLM90_02525"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMZ88271.1"
                     /translation="MGFQHNKSFGQHFLKQPETAGRIVEALSPLPEQIPILEIGPGLG
                     ILTKFLLQTEHPIYCSEIDHRIIAFLKEKLLMPSDHILEGDFLRLDIRKYFQQPLAVI
                     GNFPYNISSQILFAVLDVMELVPVVVGMFQREMAQRVTADHGNKDYGIISVLIQTWYD
                     TEYLFELPPDAFDPPPKVHSAVIKLRRKQVAPDVDPKALKNVVKTAFNQRRKKLSNAL
                     SGIPNAVPVLHQLNFAGKRAEELSVSEFLTLTKAIQAAK"
     gene            9668..10618
                     /locus_tag="PLM90_02530"
     CDS             9668..10618
                     /locus_tag="PLM90_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ88272.1"
                     /translation="MAKKRVIITDYLHSYLADWLHSQGYQVDVQPDITNEGLAEVIDN
                     YHGLALSTKIKVTPTLIDKATQLEFIARAGSGMENIDVAYAKSKGIHVVNSPEGNANA
                     VAEHALGLLLSVLNNISRADRDIRAGVWQREENRGFELMGRTVGIIGYGHTGSAFAQK
                     LRGMGVYVLAHDKYIAGFGDENVEEVSMEQIQLECDVISFHLPLNPETMHYCNAAFLA
                     KCRPGIWLINTSRGKIVNTSDLMEALDSGKVRGAGLDVFENEQFYTLAGKDKEQMEEL
                     CRRQNIVLTPHVAGWTVESYLKLSKILAEKLGALGLSATM"
     gene            10742..14458
                     /locus_tag="PLM90_02535"
     CDS             10742..14458
                     /locus_tag="PLM90_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMZ88273.1"
                     /translation="MNKLLYALVVLVLCVGSAAAQSGEIQGKVTNDKSEGIPFANVVV
                     YKNGVLQTGATTDFDGKYSIPALEPGTYEVEASYVGLKQRLTGITVSQGIVFLPDIVL
                     SEQVLQTVVVKYQAPLVDKGNTSTGGVVTKEDIQKIATRNVTSIAATKEGVYQSDEGG
                     GLNIKGSRGDATEYIIDGVRVSGSLKLPQDAIEQLEVITGGVDPKYGDATGGFITITT
                     RGPAKDYNGSIELASSQYLDDYGYNLASLFLTGPFIVKNKGTDSAVAKFGFFLAGELE
                     MEKDPDPSATDVWAVKGDVMQSLIDDPLRPSVTGAGFNKNSEFITKDDLETIGARDNV
                     NNYGLSLNSKLDYKFSKNTNLQLGLSWRKFHANTYSRNFALFNAENNPVDDQNTYRGY
                     LRFTQRFPEKRSVDNKESVVGNAYYSIQLDYTKFQSSRQDADHGMNPFAYGYIGQFNT
                     YKAPVYFYTTDETTGLTGWVLAGYADTLVEFTPGEANPALAEYTTEYINGSALPPSSL
                     FDIQLGGGLLNGDASNLFLSTYNMWWNYGVPWFNYSKSDDDQYSLNFAAALDLKNPKS
                     KKVGKHSIEFGFEFQQRVERFYGVNPIGLWTIARQLTNKHILTLDTDNPILLIDGEQY
                     TYDQYLSNPDLYFGEFDTITYNRLSLANDQAYFDAHLRDKLNSMGYAVGDLDYINIDN
                     LPLDVFDLNLFSADELLNQGSSLVFYNGYDYLGNAQTGTVSFNDFFTQEDDNGVKTRP
                     IDAFRPVYTAGYIQDRFNFNDIVFRVGVRVDRYDANRKVLIDKYSIYDVYSTAEVDGS
                     LNPNGSHPGNIGQDYVVYVDDFNSPSPTILGYRDEDTWFNADGEEVTDPNVIAVTSST
                     GTITPYVVDPDADIKDANYDPNSSFEDYTPQITIMPRIAFSFPISKVEDREALFFAHY
                     DILTQRPPTGTTTTPADYYFFLENAGALLDNPDLKPEKTIDYQVGFQQQLSTSSVIKI
                     SAFYKELRDMIQVIQVPYAYPLSYTTYGNLDFGTVKGLSISYDIARRTRNLKLSTSYT
                     LQFADGTGSSATSQINLVGAGQPNLRTIIPLSYDSRHAIKLVLDYRFENPKGSQPDWL
                     DNAGMNVTFNARSGEPYTRQSNPLPTAQFGINNRSSLEGAINGSRLPWHYRTDIKVDR
                     AFEFGLGKGERPVSMDVYLWVQNLFDAQNVIAVYPYTGNPTDDGYLTSAEGIEAISSQ
                     VNPAAFVDLYNIKMDNPDNYSIPRRIRVGVSFDF"
     gene            14484..18359
                     /locus_tag="PLM90_02540"
     CDS             14484..18359
                     /locus_tag="PLM90_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88274.1"
                     /translation="MLHKKQIWIVMLMLFLGFTAQAKENAGITGKRSTNYARLNAGCA
                     DATTQIDLDINNVRARLLGAGDFWWDLQNDAKYEIPKVDPSTGVIPVSSSFAGALWIG
                     GIDAGGQLKIAAQTYRQSGNDFWPGPLDADGTIEEATCNAYDRHWKLNRTDVDDFFET
                     ILVYGGTPGVSQTASVIPEGEIPQIIREWPGVGNQYATGKAGELLDVQKDLAPFVDVN
                     FDGIYSPELGEYPDIDGDQAIFWVYNDKGNIHTESGGDAIGIEIQAMAFGFQTNDEIN
                     DMTFYRYKVINYATTALDSTYFGQWVDSDLGAYDDDWVGCDTTLSLGMCYNGDLVDGP
                     TSPNYGANPPIFGTDFFQGPIKYITEGGSIVDSVRLGMSAFLYYNNDFSIIGNPEVAA
                     HYYGYMSGTWKDGSPFTYGGNGYGGTTLSNFIFPSDPSDPTGWSECTESNPAADRRYL
                     QSAGPFTLFPGAKNEIVVGAIWVRPPVSGGCQTSFDLARLADQKAQALFDADFQLIGG
                     PDAPDMDIRELDQSIVLSLTNGVTSNNQGESYQETDPLIVSIVSQLPDSILEANPGAT
                     DSTYNFQGYKIYQLANAQVSPSEYGDVTKAKLIYQCDQKDGIDKIVNYDFDVLIGADV
                     PELMVAGNNTGIKHTFEVTDDAFAEGTTKLINFKTYYFSVVSYAYNDFIHYDPTDPNS
                     QKRPYLEGRKNIKVYTAIPHKPDPENGGMVLNAAYGDGPEITKIEGVGNGGNFQELTD
                     ASIQTILANSFMKDPTYKGRRAPVDVMVYDPVRVPAADFELRLVDSTNSNPLNPDSTW
                     WVLTNLTEGVSVTSDFPINIVNEQVLSDWGLAVTVHNVFDPGGQTVSGSFVEKEVNNG
                     FISATLSYTDPNKQWLTGVPDNDGTLLDWIRSGIADGTFVDVLPNPDENQVFEGVLGG
                     TWSPTELVAFSTAEVPYVPFRSITGLRPQIPIEETIGVDVVFTSDRSKWSRCIVVETG
                     ECERPTGEIKLDMRQQQSVNQDGVAEGSEVGYSWFPGYAIDVETGTRLNLFFGEDGCA
                     VQPDGRGKDMCFNPSDVFFDETFNPIYGGKHYMYVSKTQYDGCENIHDSLQWIGGSKP
                     ETSVYKDIFKNIVWVSMPFLNTGYTSAVGSPMLVPTETTIQLRVTKPYSNKFVTGENL
                     GFPLYQFSTANLAPSVNDTETASAALDLIRVVPNPYYAYSAYETSSLDNAVKITNLPS
                     KCTISIFTLDGTLVRRFERDVTSDNTAGGELNSKSSNLDTSLEWDLKNEKNVPIASGM
                     YIIHIDAGDLGETTVKWFGVMRPIDLDTF"
     gene            18384..19436
                     /locus_tag="PLM90_02545"
     CDS             18384..19436
                     /locus_tag="PLM90_02545"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMZ88275.1"
                     /translation="MKRILIALTLCIPAGVFAGNPDRSGEAGASELLIDPWAQSSGWY
                     GLNIASVRGVEAPNINVAGMTFMDGSQLEFDRTEWLVGSDISLNTFGFATKVGDGALG
                     ITLTSMDLGEIEVTTTDNPEGTGAVYKPRFSNFGISYAKRFADYLSGGLTVRVISESI
                     SDVKAIGVSMDVGIQYISGSETHPEQIKMGIALRNIGTPMKYSGDGLSFRGNVPDGDY
                     SMTQEFRSESFELPSLLTIGLSYDFYFGTHNRLTAVGNFTSNSFYKDQFGAGLEYGLK
                     VKNTEMFMVRAGYRYENGITSDVDRTTASTGIAAGVTVQYPIDDLGKNLVALSYSYRT
                     AQQYQGSHCFGLRLSF"
     gene            19511..19984
                     /gene="greA"
                     /locus_tag="PLM90_02550"
     CDS             19511..19984
                     /gene="greA"
                     /locus_tag="PLM90_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMZ88276.1"
                     /translation="MAEVSYLSKEGMDNLKAEIQELKTKGRAEMARQIKEAREKGDLS
                     ENAEYDAAKEAQGLLEMRIAKLEELLANARILDESKIDTSKVSVFTTVKLQDIKFKRE
                     IKYTLVSEKEADVKSGKISVTSPIGKGLLGKKVGEVVKITVPAGVLEFKILEISM"
     gene            19986..20378
                     /locus_tag="PLM90_02555"
     CDS             19986..20378
                     /locus_tag="PLM90_02555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMZ88277.1"
                     /translation="MPSIFTRIINGEIPCHKIAENDKYFAFLDIMPLAPGHVLVVPKV
                     EVDYIFEMEEDDLAGILPFARNIAIAMEKAIPCERIGISVIGLEVPHAHVHLLPLRTM
                     DDINFSRPKLKMSQEELASIAEKIRSYL"
     gene            complement(20380..20943)
                     /gene="ruvC"
                     /locus_tag="PLM90_02560"
     CDS             complement(20380..20943)
                     /gene="ruvC"
                     /locus_tag="PLM90_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMZ88278.1"
                     /translation="MAAASEKKIILGVDPGTNILGYSIVSADKNTIKIIINGVIQLKK
                     TDDHFTRLSIIFEKLSTYIKEYQPVVMAIEAPFFGKNVQSMLKLGRAQGAAIVAAASN
                     GLEVIEYSPRRIKQSITGKGNASKEQVAKMLQSMLQLESMPKYFDETDALAVAVCHVN
                     ASKRPAVAGKQYKGWSDFIQANPGKIK"
     gene            20956..21936
                     /locus_tag="PLM90_02565"
     CDS             20956..21936
                     /locus_tag="PLM90_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HMZ88279.1"
                     /translation="MKTTNKKTLWWSIAIKVVVFGILLFALYKQVYLNENVHAIPEQF
                     LSIGAGKAIGYGILLVILMIMNWGFEAWKWKKVIDNITPIKFIRAFKAVWTGVTLGLF
                     TPNRVGEFGGRILYVPRKFRIKGAIVSLIGSFSQNLITVIAGIAGLLWYVYAIEKIDL
                     LVTGALALVAVIAVILLLLAYYNLDVMVNLFRKAKFLKRIYPYTTVLGDYSANDLSRL
                     LLLAFLRYSVYAAQYLILLNVFGAELSVMTGLAAIAVIFLAQTVVPSFAIADLLTRLP
                     VAALILSKCDVSLQISLAATTSIWILNLIIPAIAGYIFIIRFNIFKNRQS"
     gene            21933..22781
                     /locus_tag="PLM90_02570"
     CDS             21933..22781
                     /locus_tag="PLM90_02570"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HMZ88280.1"
                     /translation="MIKPLLITALSFSALVPGTNEDFSSGRVVTPSESCEIPHMAFQG
                     GESLTYKVYYNWGTLWLSGGEVYFKLTEESFKGIPVLHASGEAISYKSFDWFFKVRDK
                     ADTYMNKQTLQSYKFSRDVYEGGYTFYRSYDWDREKNIIYSYQDNHKGKKETKTIYNA
                     DPCGVDLLSTFYWIRTIDYSGLKKGDKVPVKMAIDDAQYDMYVRYDGKEVYETKLGKF
                     NCVKLKPLLQEGKVFKEGEGMTIWVTDDQNRIPVRIESDLRVGRITCDLKSYGGNKFP
                     FTSKVN"
     gene            complement(22858..23757)
                     /locus_tag="PLM90_02575"
     CDS             complement(22858..23757)
                     /locus_tag="PLM90_02575"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HMZ88281.1"
                     /translation="MRNSMILKQGDKIGLVATARWIEQETVDIAMAQISEWGFIPVVG
                     KHALSKNGQLAGTDKARASDLQMMLDDNTIKAIFCLRGGYGTIRIIDRLDFTLFTQFP
                     KWICGFSDITILHAHINDKLGLPTLHCSMPYSFPTNTSDAIAQINAILKSESITISSE
                     ATSYNKIGEASGILVGGNLSILYSLLGTRYGFSTAGKILFIEDLDEYKYHIDRMMMSM
                     KLAGKLDHIKGLIVGGMSDMKDNPVPFGMEIEEIILEHVAHLNIPVCFNFPAGHIEHN
                     LPIRLGTQAALSVTTSGGKLSFL"
     gene            23794..25872
                     /gene="metG"
                     /locus_tag="PLM90_02580"
     CDS             23794..25872
                     /gene="metG"
                     /locus_tag="PLM90_02580"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMZ88282.1"
                     /translation="MQEPKRYLITSALPYANGPLHIGHVAGAYLPADIYVRYLRKQGK
                     DVVFICGSDEHGAAITIQAKKENTTPKAIIDKYNAIISKAFSDLGISFDIYHRTSAPI
                     HHETSQEFFKTLHDKGVFEERESEQYYDEEFKQFLADRYIKGTCPVCANPNAYGDQCE
                     KCGTSLSPTDLIEPVSTLSNKPPVKRKTTHWYLPLNKFQDWLQSWIVEGEGRTEEWKK
                     NVLGQCASWLKEGLHPRAITRDLDWGVKVPLPNSEGKVLYVWLDAPIGYISATKQWAL
                     NTGKDWEPYWKSADTKLLHFIGKDNIVFHCLIFPCILKSHGEYILPTNVPANEFMNLE
                     GDKMSTSRNWNVQVHEFLEEFPGKEDVLRYVLCANMPETKDSEFTWKDYQARNNNELV
                     AIFGNYANRVMVLTEKYYGSKVPAQVDSVEQDAYVVAYRAQVAQLFNDYCTGIESYRF
                     RDALMAFMDIVRLGNKLLTDFEPWKLYKTDPIRTEGVLRICLESLYDMATLCEPLLPF
                     SAGRLNAYFNLPAAPKMAVGGVNLTSGAGLGSPFYLFEKIEDQVIQSKLDALESIRRS
                     REAVTAAETTIIAANPIKEAISYDDFSKLDLRSGVVLSAERIAGADKLLKLTVDLGFE
                     VRTIVSGIALHFSPEALTGKKVVVLANLLPRKMRGVESNGMILMAEGKDGALHFVAAE
                     GNAGPGDSIS"
     gene            26037..28433
                     /locus_tag="PLM90_02585"
     CDS             26037..28433
                     /locus_tag="PLM90_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HMZ88283.1"
                     /translation="MKKFSSITLLLLVVGFTQTLYAQSTPIVPGVSSSTLESLGFDSA
                     EIDAIMSTTGSSTSTAVEAAAQIQDAQQSAVQAANNQLNTLAPNTATLPPPVVEQVIE
                     QAISNDTPSSAPAGIWGQDIFRNGSIDLFDKVPNAKVSDNYIIGAGDQINVSIYGWAY
                     YNGNLTVSEDGYITTVETGRIYLKGLTFSAARQLVRQRFASSFDLVHSTFDMTLTYSK
                     TIKVSIVGEVVKPGTYNISSVNTAFNALVAAGGVTDIGSVRNIQVKRNGKVIKTLDVY
                     QFLMNPGSTDDTYLEANDYIIVNGIGRVVDVQGEINRPLKYELVKGENLNELIAYAGG
                     LRSTAYKRNVTIFRYINNENVVLDFNLDSLERERVNFPLLDGDKITFARIPEVVENIV
                     TIQGSCRFPGNYQLTEDMRISDLIAKAKGLNYDAYTDRAYLIRKNDKLNDVYIPFDLQ
                     EVIDNPNSAFNFKLTKFDVIDIFSKQEFRETFNVSISGSVKVPGIFPYYEKMTLKDLL
                     YYSGGLKVEAANSRIEISRIVNFNEANSDNEPTRVVIQSISIGKQLELEDGANTFQLQ
                     PYDQVFVRNTPEFEYQKNVTVLGEVKFPGVYTLTSRTETLADVIERAGGLTQWAYAEG
                     ATMNRNDVASTLVFLHKALENPESKYNYVLREGDVITVPKAGDLVALKGEIKYPFVGD
                     PGTVIVPFEKGRSARFFIKKYGKNFDDDAKRSETYIVEPNGYVRRTHNLLFIHFYPRV
                     KVKGCQVVVPQKPEEKPDAPEAPSAPREPFDWNTFATTLSAGILSFATIYVLILKVPS
                     "
     gene            28464..29609
                     /locus_tag="PLM90_02590"
     CDS             28464..29609
                     /locus_tag="PLM90_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88284.1"
                     /translation="MQYDRPYNDEINIGDLFTKFGEYRRYLVKKWWVILIAAILLAIG
                     LRFFAVWKKEYYISHADFAVKGTEGSSTSSLASLASSFGIGITTGTEFTNELFLGILQ
                     SRTLVKEALMEKRTLTVKKGVAPREDYLANFYVEMYPRWAKKKKVKNFKLEHGSLDSL
                     TVYEDSCLTVIYDEIIENDISTEFSDDLGMNQMEVSSLSRDFSYYLADYLAYAGSNYY
                     INAQVVNEQKTVNLMSEKADSLRALLDGKEELLARIQDGSAYQVKLSGYVNQNRLIRE
                     IGLITTEYSTTYAQLQLALFDLQNKTPLVDMIDQPKFATIKEKEQTTMFMIIGFILGT
                     ILTSIVLAFRKYIRDSVEEARQKQLVIEKYHKEQESQSLIDTDSNKI"
     gene            29636..31135
                     /locus_tag="PLM90_02595"
     CDS             29636..31135
                     /locus_tag="PLM90_02595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ88285.1"
                     /translation="MIFNSLQFLIFLPVAIFLYFSIPYKWRWLFLLFSSYYFYMSWKA
                     EYAFLIVFSTTVDYLTANAMAKQTDKRKRRPYMLISLFSNLGLLFSFKYFNFFNDATR
                     AVFDSFDIFYNVPEFNLFLPVGISFYTFQALSYTIDVYRGDVKAEKHFGYFALFISYW
                     PQLVAGPIERPGDLQPQLKSNHDFDYDRVKKGLTRILYGFFKKVVIADRLGIFVQAVY
                     GQSNGMGGWNYPSFEHGGWPVVIATWLFAIQVYCDFSGYCDIAIGSAKIMGHNLTDNF
                     RTPYFSKSIREFWERWHITLTVWVRDYLYIPLGGSRVSFGRMLFNNWFTLTVMGFWHG
                     ANLTYVMFGFIHGFYIVVSRVRDKYMPKMVLAKWIPNMPILVNVLLMIWIFNLTCIPD
                     IFFRSNSISDAWHIIGQMFKASTMHEFIYNSAPKGAVPSVIEFWISLVFIAMLLFIDY
                     NLYIRKSATIEDQVAIRPWYTRWSFYVIFLVIVACFAVTTKSAFIYFAF"
     gene            31139..32269
                     /locus_tag="PLM90_02600"
     CDS             31139..32269
                     /locus_tag="PLM90_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88286.1"
                     /translation="MKKLILRIGLLLVIVFAICQLILMSMPFSWGNTRLNTKYTTYSE
                     NPEQYNTLLIGASTTYRHIDPKQFDAAVNAQYPDLHIKSFNFGIPANRTPQSMYMLEN
                     LLKEGNTDHIKYVVIDLSELTKMGADNLHKKEMLYWYNWGNIGDVMKASYESEKGTAN
                     KFGVPFLHAFSFGEKSFLVGMGPSVIMQHAGLNIEPLSVGPDKNGFYSLDQEMHDDPE
                     GDLAIRYNDLRTQDTIDFRTQRCQMLYDKFKDAKKNPNKTMEADLLKIIKDCEKQDIS
                     VVIMLSQRLGDRYEYLIPLYNRIPEKNRIGFQNPSAYPMFNERENLFDLAHLNSSGAK
                     LFTDIFADQWIDRLIMQGKIAPRPVAPLNSLQQTNSGTGDAL"
     gene            32266..>32559
                     /locus_tag="PLM90_02605"
     CDS             32266..>32559
                     /locus_tag="PLM90_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ88287.1"
                     /translation="MIRKITKKFRRKLKDQDFKEIFSKGISGTAISLVGRFSGYLLLM
                     VIGWVYGQEAMGVYTVGLSILSIVGIFGRFGVDMAMSRFVAQYGGSGRWDLIRH"
BASE COUNT         9293 a         6541 c         6986 g         9739 t
ORIGIN      
        1 ttggacattc aggtgagaga tcagcggttg gataggcaat atttctttct gtgcgatata
       61 aaattagcaa ctcctcatcg tcgatttgtt caaatgtatt gtagaaatag agtatgttgc
      121 catcgggttg cggcagcata tcttcgcttt gcgaatacgg atgtggaccc caagtgggat
      181 caatatcgaa aaaattctca ttcccatcta tatcaaaaca ttgcaatgaa cactgatcgg
      241 gagaaaatat acgtaagcta aatgtgatat acccgttcgg atcaaggtag tgctccggta
      301 aggtgcagct ggtagttgta gtgcccaatg aatatgtatt tacgaagtcc aactcaaacg
      361 ggtcatcgtc attgaatcga caggtaaaaa aggcgtcttc gacaagatca aaaaatgcta
      421 attgaacgcc tttgcaaggc acatattgat aatcaggctg tgctaaaaaa tggcccgtaa
      481 gcagttcgct attacgttcc aatacaccaa gctcatttaa ttgtagttta taattttgca
      541 gatacagata aatatcattt tgagcatcta catgaatgaa atcgatcgca ctattttcat
      601 ttgaaactgt ttttgcaaga tcatcataca accaaagctt gtttccatcc ggagataatt
      661 ttatcagacc ggtatattta taattgtaca atgtatgccc aataccatca tcctcccagg
      721 cataaacatt atgtccaagg taaatatttt gaagatggtc aacttgcacc attgtaaact
      781 ccgtattata ctcagtatcc tcatatgttc caatgatgtt agtattttcg tccagaatta
      841 aaattttcgg atggggataa ttatcataag ggccacctac cgtatttaag attatacgat
      901 catatttgac aaagagcgac ttgtattcat cagtgtaagt gcgttttgcg aatatatctc
      961 cattcgaatt gaaaatgtat aaagtgctgc tatctgcata ttgaataagc aaatgggctt
     1021 tatccttatg aagagcaatt tgaaccccga aattcggagc aaatttttga taccaaagga
     1081 tgctgtgatc aggcccatat ttaattaaat ataaactgtc agatcccgtt gcgcatagca
     1141 gataatagtt gccattctca gcacccatag cctcaagttg ataggctagc agttttccat
     1201 cgcaataaag cgcttcctgg gctttgcttg tcaggatata gcagaatagg ctaattgtca
     1261 gaataattgg tttcatgttg gggttttaag ctaacagaat aacgatgtgt aaatataata
     1321 attgcgattt aatggccagt tgcgaactta caagctgtat ggaattgtaa tgccttgtgt
     1381 tttgtaatgc attgaaattg aatgactttt gaatttagct tgttatcaaa tatactacgt
     1441 gtctcttttt ttgcgttaca aatattttct tttatcttta ttttgtttca ggcgcaagtg
     1501 attaatactc ttgtttacat gccaagcctg ctgtatttga ctaataaact tacacccaat
     1561 gaaaattatt ttgaattgct ttttcttgat atgtatcact tcagttggat atagtcagac
     1621 ctttgaaaaa gtttacgaaa agagatattt ctttgcttgt gagtcgttgg ataatagtta
     1681 tttactggtg ggtgaagatc cggtcgatga tacacttgac aatatcagtt tattgcgaat
     1741 ttcggcaatt aacggtaatg tccaatcgga atatatctat tcacttgaag gattcgattt
     1801 atttccaaac tttgttattg aaaccgatga cggcggtgca tacatttgtg ccattttgag
     1861 atacgacaat ttacatggcg acccaatcat aataaaaatt gacgcctgtg gcaatccatt
     1921 atggatgcga aatttgaatg aaatattctg cccttatctt ggtctttcct atttgaatga
     1981 ggtaaattat gcaacattag acggtaatgg tggactaacc tttgtgattg gcttggagtg
     2041 tgtatccttc tattgcttta atattgatca gtatggcata gtaaggtggg tcagtaacgc
     2101 gattggtgaa gagtcgtgga tgatgagtgg caccaatttt cgccgcatgg atgatggtaa
     2161 atatgttttg accgggtatc aacatactta ttggtccgac actgtattat atagttcaga
     2221 atataaaata aacgctgcta ttcgaattct tgatagtata ggaaattttg tcgatgaaga
     2281 ggaatatatg ttggattcat tcgaaagtat ggcttacggt atatgcgatt cttgggtaag
     2341 tggtcagtat gttggaactt gccataccgg agtggagcac tataccatac ttgcagtatc
     2401 taatgaattg gattctatct cgcattatga ggttgcctcc atgtatgatt atgttctgtt
     2461 gccgttggac atatatcaag gacctaaagg acaatattat atcataggaa attcttgcga
     2521 tactatccca ttattacata gcggattggg agcccaatta atcgatagtt atggtaatgt
     2581 gcttgctcaa aatagcaccg ggtttgacat caattatgga taccggaata gttttgttac
     2641 ctctgacctc aacgtagtaa tcttggcaga tgttcgagat gattctgaaa ctgcggaatc
     2701 agaattcgat ttggatacct acctcatcag ttttgatcag gatctcaata tttcattgat
     2761 aacagatacc gttacgcctg tcagtgctat ttcggaagct tgcggatatg aaactgcgac
     2821 tgtacatttc gatctgtcgc ctatcatgga agagggcctt gcttatacac ttccggattc
     2881 tagtttgatc attcatgagg aagctgataa agcaaatgct aaaaattggg gagctatcaa
     2941 tgtttatcca aatccaatga ctgacaaggc actaataaca tgggaaaaag aagatccggt
     3001 tgttggtatt catgtatatt acccaaatgg ttccctcctt acttctgttg aaattggaga
     3061 agcgattcaa tattggcagt ttgatcgagg taacttggca tcaggcctat acgcttatat
     3121 tattattaga actaatggaa gtcaaagcgc aggattgatt gtcttgcaat aaggataacc
     3181 ctaatagaaa ttattgaatg gcgtgtaaat aaaaaaacca cccgacatgg atggtttttt
     3241 taatatgttc agcatttgct taatctccca gattactcaa atcgaaacta ttcataaatg
     3301 aagtatcgaa cttcccggca ataaagcgtt catcgcgcat gagttttttg tggaaaggaa
     3361 tagttgtttt aataccttca acaataaatt catcaagtgc tcgctgcatt tttaaaatta
     3421 cttcctgacg cgtacgtgct ttacaaatga gtttcgcaat catagaatcg taatactgcg
     3481 gaatggtata tccggcatac acatgtgtat ctacgcgaac accatgtcct ttcggtgagt
     3541 ggaaactggt aatgcgtccc ggacttggtg caaagttcct tgaaggatct tctgcattga
     3601 tgcgacattc catagcgtga agttcaggga aataattttt tcctgatatt ttctcgccca
     3661 tggcaatttt tatttgttct ttgataaggt cgaaatcaac aacttcttcc gttacagggt
     3721 gttctacctg aatacgggta ttcatttcca tgaaataaaa gttgcgatgt ttgtcagtta
     3781 gaaattctat ggttcctaca ccttcatatg ctatactttc tgcagctaaa acggcagcgt
     3841 cacccatttt ctgacgcaat ttatcatcca taaacggtga agggctttct tcaaccagct
     3901 tttggtgacg acgttgaata gaacaatcgc gttcgctgag gtgacaagct ttgccatatc
     3961 ggtcacctgc cacttgaatt tcaatgtgac gtggttcttc gatatatttt tccatataaa
     4021 ggccatcatt actaaatgat gctttcgcct ccatactggc agtattaaat gcattttcca
     4081 cttcactttc attccacact atgcgcatgc cttttccacc accgccggca gtagctttca
     4141 tgattaccgg ataaccgatg cgtttagctt gcttcttagc atcggctata tctttcagca
     4201 atccttcgct acccggaatt accggaacac ctgctttttt catcgtttcc tttgcagtaa
     4261 tcttatcacc catttggtgg atctgctcag gcattggtcc gatgaattta ataccatact
     4321 tggcacaaat tgccgagaac atctcattct cacttaaaaa tccatatccc ggatgtatag
     4381 catctgcatt ggtaatttct gcagcagcca taattttagg aatgttcagg tatgattctt
     4441 ttgaagatgg cggaccgata caaactgctt catcagcgaa acgaacaggt aaactgtctt
     4501 tatcggctgt agaataaaca gctacggttt taatacccat ctccttcgcc gtacgaataa
     4561 tacgcagggc gatctctccg cggttggcaa tcaggatctt tttaatgggc ctgatcaatt
     4621 cctgcggttt actgctcttt ttggtcatgc gaaaaccgtt tattatttag gctcgatgag
     4681 gaataaaggc tgatcatatt ctaccggtga agcatcgtcg gcaagtattt tcaccaagcg
     4741 accacttact tcactttcga tctcattgaa caatttcatt gcttcaatga tgcagatctt
     4801 tgaacctaca gagatttcat ctcctacttt aataaatgct tctttatcag ggccgggtga
     4861 acgatagaaa gtgcccacca ttggagattt aatcgtgatg gtattaccac ctgttgattc
     4921 agcagcatcg ctctttgttt cagctgcagc catttgtggt ttttcaggtg ctgcactcat
     4981 ttgaggcatc atcacaggct gttgcatctg tggcatcatc ataggcgcct gaaccattgt
     5041 ggttgcagca agcgctgttt tgatcttgat gcgaaaatct tggttttcga tagtgagctc
     5101 agcaacattg tgcttgttca ccattctgat gagctcttga atttctttaa agtccatacg
     5161 tgttatttta cgcgttcaac atattcaaat gtgcgggtgt cgattttaat aacatcacct
     5221 tcttcaacaa acaacggcac ctgaatgcgt gcgcctgtct caacagtggc gtatttcatc
     5281 gcgttggtgg cggtattccc tttcaccccc ggttctgcaa atgtaacagc taaaataata
     5341 tgggcgggca attcgcagaa taaaggtttc tctgtgtcgg catggaacag gatgtcgatc
     5401 tcctggcctt ccttgaaaaa ctcttttcca tcaaccattt cgccgtctaa ggtcacctgg
     5461 tcgaaggtct cgttgttcat gaagtggtaa cctgtttcat cgttatacaa atactgataa
     5521 gtgcgacgct caacccttac cggattgatg gttgcacctg atggaaaggt gttttccaat
     5581 acccttgcag tggtaaggct cctgagcttg gtgcgcacaa atgcattgcc cttgccgggt
     5641 tttacgtgtt ggaagtcgat aatctgccag atgtcgtggt tgaaatcgat acaaagacct
     5701 tttctgatat ctgctgttga agccatatta aaatgattga gtgattgctt gtttcaaaat
     5761 gtcctgcgtg aattcttcgc agctatatta tacacattcg ctttgtattg attagccggc
     5821 ttttaacgtg cttttcagct gttgtaagcc catttcacaa gggttgcacc ccaagtgaat
     5881 ccaccaccaa aggcagctaa cacaatatta tcgccctttt tcaactgcgg ctcccattcc
     5941 cataaacaaa gcgggatggt tccggcagta gtgttgccat atttttgaat attaatcatg
     6001 accttttctt tcgaaaggcc catgcgctct gcggttgcgt cgataatacg caaattggcc
     6061 tgatgtggta ccaaccatga aatatcatcg ctcttcagat tattgcgtaa catgatctcc
     6121 tccgcagcat cagccatgtt tttcacggcg aatttgaata ccgtttttcc ctcctgatat
     6181 acggtatgct ccatattatc taccgtctcg tgactggcag gcttgagaga accaccggct
     6241 ttttggtgga ggaaatgacg accagagcca tccgatttca gcaccgcatc agcaatgccg
     6301 ttaccgtcag tgctaggttc cagcatcaca gcaccagcgc catcgccaaa aatcacgcaa
     6361 gtggcacgat cggtatagtt gatgatgctc gacatcttgt cggcaccaac aacaactact
     6421 ttcttatact ttcctgattc aatgaattgg gcagctgtgg acagggcaaa aagaaagccg
     6481 ctgcatgccg cgctgatatc gtagctgaga atatgattac atcccagttt atcacacgca
     6541 atattggccg tagcggggaa ttgcatatcg ggcgttgtag ttgccacaat aagcatctcg
     6601 atatcaccgg cagtgttgcc cgttttagct aacagacctt tcacagcctc aactaccata
     6661 tcgctggtag ccaggccctc tcctttgagg atacggcgtt cgctgatgcc tgttctggtt
     6721 aagatccact cgtcggtagt atctaccatg cgctccaatt cggcattggt tagccgatat
     6781 tcaggaacat agccatacac acctgtgatt gctgcaaggg ttttctgcat acctggttta
     6841 gcttaataag gggttagtcc ccaatatttc aggctttttt ctcaatggcg atcttgcctc
     6901 tgtaagagcc gcattccggg catacacggt gaggtaatac cggagcgcca caattagcac
     6961 atgctgaaac gttaggcatg tcaactccgt ggtgcgtcct gcgcgacctt gtcctggttt
     7021 tggaatggcg tcgttttgga tttggcattg tattctgttt taattgatat tcaaattatt
     7081 caatatatcc catcttggat ccggatccgg acctttttca tcctgcgatc cttcttcttc
     7141 ctgccctaat aactttaaaa tttcaggatc acagcccgga actcctccgg ccttgtcggg
     7201 atgcgctttg agcataggca ccgataagtg cacaaactca taaaccatct ctgaaatatc
     7261 cagtatcgac tcactgtcgg gaatccaaac cacctcctca ctatctacat cctcctcccg
     7321 cttatcacct aacttcacat aaatggtgta attaccatga ataggaaggt caaatggctg
     7381 tccgcagcga tcgcaatcgg tactgatact acctgaaaca tcaaaattca gaacaaaaag
     7441 tcgctctttt ttatccagtg cgaggtcgac aaataccttc ccctgctgca cctgactctc
     7501 gggaaaatgc ccgaagaata catcgtccaa ctcatagttg aagtggtgta ccccttgctt
     7561 caaccccaca aacgggattt catattccct cagatcgttc gccaccggat tcttaaaaag
     7621 cgaacaaagg tagggaagtt ttccgaagat tggaagcctt cctaacaggt atttgaagat
     7681 aaataccctt taaaatttac tcaaatgaag gttttttcac cccaaactgc cttaagcgcc
     7741 tctttcactg gcaacttccg tgcgtttcaa agggttggca gtgatctcat gatatacttt
     7801 gcgactgcga acaatatcta cggctgtaaa tatagcctga agcatgctct cagcagaagc
     7861 ctcattcttg cctgcgatat caaaggctgt tccgtgatct ggtgatgttc tcacaaatgg
     7921 aagtcccgca gtaaagttca ccccgccgcc aaaactcaga ctcttgaacc ctacaagtcc
     7981 ctggtcgtga tacatggcta aaatcgcatc aaagtgctgg tattgcccgc ttccaaagaa
     8041 accatcggca ctaaatggcc cggttacaaa cattttcttt tcctgagctg ccttgatagc
     8101 cggaataata atattgctct cttcagcacc caaagttcca ctgtcgccgg catgtggatt
     8161 caaccctaaa accgcgatgc gcggacctgt tgacgcaaaa tcctgcttca ggctgtgatg
     8221 aatgatctcc agttttctga gcaagccttc cttagttaag gctgaagcta catccttcac
     8281 cggaatatgt cctgttgcca atccaacgcg cagatcgcct tgcatgagta acataacact
     8341 ttcgctttca ggtgcttttg cattaaaata ttcggtatgg ccggggtaat tgaagcctat
     8401 ggattgtaga ttgtgtttgt tgatgggggc cgttacaatg gcatcaagct gtcctttcaa
     8461 cagatcattg gtagctgcct ccaatgatgt tagtgctaca cgcgctccgg tttcattttc
     8521 ctgtccgggc ataatcatca cctcttgtgt aatacaatta atgatgttca ccgccttagc
     8581 attagcctga tcggcactgt taattgttgt ataactcaag ccttcgagat tgaagagctt
     8641 tttataaaaa gaaatgatcc tgttcgatcc atagatcaca ggtgtacata aaccaagcat
     8701 tcgcttatct tccaatgcct taatcaccac ttcagggcct ataccgttca catcacccaa
     8761 cgtaaagccg attctgatcc tattttccat aatcctttca caaaagtaag catttcaaaa
     8821 cggtatagtg cactctttca cgttgccggc cattacattt gaaacattac ctctaatatt
     8881 gcatttgcaa taatgggatt tcaacacaac aaatcatttg gtcaacattt tctaaaacaa
     8941 cctgaaaccg caggtcgtat tgttgaagct ttgtcgccac tgcctgaaca aattcccatt
     9001 cttgaaattg gtcccggctt aggtattctt acaaagtttt tattgcaaac ggaacatccc
     9061 atttattgca gtgagatcga tcatcgaatt atcgcttttc ttaaagaaaa attactgatg
     9121 ccatcagatc atattttgga aggcgatttt ctgcgactcg atatcaggaa atattttcaa
     9181 cagcctttag cggtaatagg caattttcca tataatattt cttcacaaat attatttgcc
     9241 gtactcgatg tgatggagct tgtacctgtt gtggtgggca tgtttcagcg cgaaatggca
     9301 cagcgtgtta cagcagatca tggcaataaa gattatggca ttatcagtgt attgatacaa
     9361 acatggtacg ataccgaata cctttttgaa ttaccgcccg atgcatttga tccaccaccc
     9421 aaagtgcatt ctgctgtgat caaattaagg cggaaacaag tagctcctga tgttgaccca
     9481 aaggcattaa aaaatgttgt gaaaacagct tttaatcagc gcagaaaaaa attgtcgaat
     9541 gcactatcag gtattcccaa tgctgttcct gttttacatc aacttaattt tgcaggcaag
     9601 cgtgctgagg aattaagcgt ctctgaattt ttgaccctta caaaagcgat tcaagcagca
     9661 aaataatatg gcaaagaaac gcgtgattat tactgactac ctgcatagtt atctggccga
     9721 ctggttgcat tcacaaggct atcaggtcga tgtgcagcct gacattacaa atgaggggct
     9781 tgcggaggtc attgacaatt accatggatt agcattgagc accaaaatca aggtcacacc
     9841 cacccttatc gacaaggcca ctcaactcga attcatagcc agagccggaa gtggaatgga
     9901 aaatattgat gtggcctatg caaagagtaa gggtattcat gtagttaaca gtcctgaagg
     9961 caatgccaat gctgttgcag aacatgcact gggactgctt ttaagcgtat tgaacaatat
    10021 ttcccgtgcc gatcgcgata tccgtgccgg tgtatggcaa cgcgaagaaa atcgaggttt
    10081 tgagctcatg ggtcgtacgg tgggcattat aggctatggt cataccggta gcgcctttgc
    10141 ccaaaaactg cgcgggatgg gggtttatgt gcttgctcat gataagtata ttgcaggttt
    10201 tggcgatgaa aatgttgagg aagtaagcat ggaacagata caattggaat gtgatgtcat
    10261 cagttttcac ctgcccttga atccggaaac catgcactat tgcaatgcgg ccttcctggc
    10321 aaaatgtcgc ccgggcatat ggctgataaa cacctccaga ggaaaaattg tcaacacctc
    10381 cgatctcatg gaggcactcg acagcgggaa ggtgcgaggg gcaggtctcg atgtattcga
    10441 aaatgagcaa ttctatacat tggcaggcaa ggataaggag cagatggagg aattgtgtcg
    10501 ccgacaaaat attgtcctta ctccgcacgt tgcaggatgg acggtagaat cgtatcttaa
    10561 attgtccaaa atattagccg aaaagcttgg ggcactaggg ttatcggcta ccatgtgacc
    10621 taaatatgaa gttattgtga cgaggttatg acgcacatat aaattccttc cactaccttt
    10681 gttttcaatt atttacatat tttagcgact cttaacacaa tttaaataaa catttcggtc
    10741 tatgaataaa ctcctttacg cactcgtcgt actagtattg tgtgtgggat ctgcagcagc
    10801 gcagtccggt gagatccagg gaaaggtaac caacgacaaa agcgaaggta ttccctttgc
    10861 caacgtagtt gtttacaaga acggggttct ccaaaccggt gctaccaccg actttgacgg
    10921 taagtattca attccggcgc tcgaaccggg aacttacgag gttgaagctt cttatgttgg
    10981 tttgaaacaa aggttgacag gcattacggt atctcaaggt atcgtctttt tgccggatat
    11041 cgttttgagc gagcaagtgc ttcaaacggt agtcgtgaaa tatcaggcac cattggttga
    11101 taaaggaaat accagcacag gcggggtagt tacgaaggag gatatccaaa agatcgccac
    11161 ccgtaacgtt acctccatcg ctgcaacaaa agaaggtgta taccagagtg atgaaggtgg
    11221 cggcttgaac atcaaaggtt cacgcggcga tgctaccgaa tatatcatcg atggtgtacg
    11281 tgtatcagga tcactgaaat tgcctcaaga tgctatcgaa cagctcgagg ttatcaccgg
    11341 tggtgttgac cctaaatatg gtgacgcaac aggtggtttc atcaccatca ccacaagagg
    11401 tcctgccaaa gattataatg gaagtatcga gttggcatca agccagtatc tcgatgatta
    11461 cggatataac cttgcttcat tattcctgac cggtcctttt atcgtaaaaa ataaaggcac
    11521 tgattctgca gtagctaaat ttggcttctt ccttgcaggt gagctcgaaa tggaaaaaga
    11581 tcctgatcca tcggctactg atgtttgggc agttaagggt gatgtgatgc aaagtcttat
    11641 cgacgaccct ctgcgtcctt cagtaacagg cgccggcttc aataaaaatt ctgaattcat
    11701 cactaaagat gatcttgaaa ctattggtgc acgcgacaat gtaaataact acggactttc
    11761 attgaactca aaactggatt ataaattcag taagaatact aatcttcagt taggattgtc
    11821 atggcgtaaa ttccatgcta atacttattc aagaaacttc gcattattta atgcagaaaa
    11881 taatccggtt gatgatcaaa atacttaccg cggttatctt cgttttaccc agcgctttcc
    11941 tgaaaaacgc agcgttgata ataaagaatc agttgttggt aatgcatatt acagcatcca
    12001 gctcgattat accaaattcc aatctagtcg tcaggatgca gaccatggca tgaatccatt
    12061 tgcttatggt tacatcggac aattcaatac ttataaagca ccggtatatt tttataccac
    12121 tgatgagact accggtctta ctggatgggt gcttgcaggt tatgccgata cgctggtaga
    12181 atttactccc ggtgaagcaa accctgcact cgcagaatat actacagaat atatcaatgg
    12241 tagcgcattg cctccatcaa gcctttttga tattcagtta ggtggtggtt tattaaatgg
    12301 tgatgcatca aacctgttcc tttctactta taatatgtgg tggaactatg gtgtgccttg
    12361 gttcaactac agcaaatctg atgacgacca atatagcctc aactttgctg ccgcactcga
    12421 ccttaaaaat ccaaaatcta aaaaagttgg gaaacacagt atagaattcg gttttgaatt
    12481 ccaacaacgt gttgaacgtt tttatggcgt taacccaatt ggtttgtgga ccattgcacg
    12541 tcagttgaca aacaaacaca ttctcacttt agatactgat aatccaattt tattgatcga
    12601 tggtgagcaa tacacttacg atcaatatct cagcaatcct gatttgtatt ttggtgaatt
    12661 tgataccatc acatacaatc gtttgagcct tgctaatgat caagcttatt tcgatgcgca
    12721 tcttcgcgat aaactaaata gcatgggtta tgctgttggc gatcttgatt atatcaatat
    12781 cgataattta ccactcgatg tttttgatct taatttattc agcgccgatg aattactcaa
    12841 tcagggttct tcactggtgt tctataatgg ttatgattat ttaggtaatg ctcaaacagg
    12901 aacagtttcc ttcaacgact tctttacgca ggaagacgat aatggagtta aaacacgtcc
    12961 aatcgatgcg ttccgtcctg tgtatacagc aggttatatt caagaccgct tcaatttcaa
    13021 cgatatcgta ttccgcgtag gtgtgcgtgt tgaccgttac gatgcaaacc gcaaagtgtt
    13081 gatcgataaa tattctatat atgatgttta cagcactgct gaagttgatg gttctttgaa
    13141 tcctaatggc agccatccgg gcaacatcgg tcaagactat gttgtttacg ttgatgactt
    13201 taactctcct tcaccaacta ttcttggtta tcgtgatgaa gatacctggt ttaatgccga
    13261 tggcgaagaa gtaactgacc ctaacgtaat tgctgttaca tcttcaactg gtactatcac
    13321 accatatgta gtagatcctg atgccgatat taaagatgct aattatgatc cgaattcatc
    13381 attcgaagat tatactccgc agattactat catgcctcgt atcgcattct ctttccctat
    13441 ttctaaagtg gaagatcgtg aggcattgtt ctttgctcac tatgatatct taactcagcg
    13501 tcctccaaca ggaacaacaa caacacctgc cgattattat ttcttcctag aaaatgcagg
    13561 cgcattgttg gataaccctg atttgaaacc agaaaaaaca atcgactacc aagttggttt
    13621 ccaacaacag ttgtctactt catctgtaat caaaatatca gcattctaca aagaattgcg
    13681 cgatatgatt caggttattc aagtgcctta tgcatatcca ctgtcataca ccacttatgg
    13741 aaacctcgac tttggaacag taaaaggact ttctatttct tatgatattg ctcgtcgcac
    13801 acgcaacttg aaattatcta caagctatac attgcagttt gcagatggaa caggttctag
    13861 tgcaacatca cagatcaact tagttggtgc aggacaacca aacctccgca caattattcc
    13921 attgtcatat gattcacgtc acgcgattaa actcgtgctc gactatcgct ttgaaaatcc
    13981 aaaaggcagc cagcctgatt ggttagacaa tgccggtatg aacgttacat tcaatgcgcg
    14041 ttcaggtgaa ccttatacgc gtcagtcgaa cccattgcct acagcacaat tcggtatcaa
    14101 caaccgttct tcacttgaag gagctatcaa tggttcacgc ttgccatggc actatcgcac
    14161 agatattaaa gttgaccgtg ctttcgaatt tggcttaggc aaaggtgaac gccctgtttc
    14221 aatggatgta tatctgtggg tgcaaaacct tttcgatgca caaaacgtga ttgctgttta
    14281 tccatatacc ggtaacccaa ctgatgatgg ttatttgaca tcagcagaag gtatcgaagc
    14341 gatcagctca caggtaaatc ctgcagcatt tgtcgatttg tataatatta aaatggataa
    14401 tcctgataac tacagtattc cacgcaggat ccgtgtaggt gtttcatttg atttctaatc
    14461 cgaaaaaaag taaactggta gatatgttac ataaaaagca gatttggatt gttatgctca
    14521 tgcttttcct gggctttacc gcacaggcaa aagaaaatgc agggatcacc ggtaaacgta
    14581 gcacgaacta tgcgcgtttg aatgccggtt gtgctgatgc aacaacgcag atcgatctgg
    14641 atattaataa cgttcgcgcc cgcttgttag gggcaggcga cttctggtgg gaccttcaga
    14701 atgacgcaaa gtatgaaatt cctaaagttg acccttcaac aggtgttatt cctgtaagct
    14761 caagctttgc aggcgcactc tggatcggtg gtatcgatgc cggtggtcag ttgaaaattg
    14821 ccgctcagac ttatcgtcaa agtggtaatg acttttggcc gggaccactt gatgccgacg
    14881 gaactattga agaagcaact tgtaatgctt acgaccgtca ctggaaactc aaccgtacag
    14941 atgtagatga tttctttgaa accattcttg tttatggtgg aactccgggt gtttctcaaa
    15001 ctgcatcagt aatccccgaa ggtgaaatac ctcaaatcat ccgcgaatgg ccgggagtag
    15061 gtaaccagta tgctaccggt aaagcaggtg aattgcttga cgttcaaaaa gaccttgctc
    15121 catttgttga cgtaaacttc gatggtatct attctcctga attaggcgaa tatcctgata
    15181 tcgatggcga ccaggctatc ttctgggttt ataacgataa aggaaacatc cacactgaat
    15241 caggtggtga tgctatcggt atagaaattc aagctatggc atttggtttc caaacaaacg
    15301 atgagatcaa cgacatgacc ttctatcgtt ataaggttat caactatgct actacagcac
    15361 tcgactcaac atatttcggt caatgggtag actccgactt gggagcctat gatgatgact
    15421 gggttggttg tgatacaact ttatcactcg gaatgtgcta caatggcgac cttgttgatg
    15481 gtcctacatc tccaaattat ggtgcaaacc caccaatctt tggaacagac ttcttccagg
    15541 ggccaattaa atacatcact gaaggcggtt ctatcgtaga tagcgtgcgc ctcggaatgt
    15601 cggctttcct ctattataac aatgacttct cgataatcgg taaccctgag gttgctgctc
    15661 actactatgg ttatatgtca ggaacctgga aagatggttc tccatttacc tatggtggaa
    15721 acggctacgg tggaacaaca ctttcaaact tcatcttccc tagcgatcct tcagatccta
    15781 caggttggag cgagtgtact gaaagcaatc ccgctgccga ccgtcgttat ctgcaatctg
    15841 ccggtccttt cacactcttc ccgggtgcga aaaatgagat cgtagttggt gccatttggg
    15901 tgcgccctcc ggtatcaggt ggttgtcaga cttcatttga ccttgctcgt ttggccgacc
    15961 aaaaagcaca ggcattgttt gatgccgact tccagttaat tggtggtccg gatgcacctg
    16021 atatggatat ccgtgagtta gatcaatcga tagtattgtc tttaacaaat ggtgttacgt
    16081 caaacaacca gggtgaatca tatcaagaaa cagatccact gattgtaagt atcgtttctc
    16141 agcttcctga tagtattctt gaagcaaatc cgggtgctac tgattctact tataacttcc
    16201 aaggatataa aatttatcag ttggcgaacg ctcaggtttc tccttctgaa tatggcgatg
    16261 taacaaaagc gaaattgatt tatcaatgcg atcagaaaga tggtatcgat aaaattgtga
    16321 actatgattt tgatgtattg ataggagctg atgttcctga attaatggtt gcaggtaaca
    16381 acacaggtat aaaacatacc ttcgaagtta ccgatgatgc attcgcagaa gggacaacga
    16441 aattgataaa tttcaaaaca tattatttct ctgttgtatc ctatgcatat aatgatttca
    16501 ttcattatga tccaacagat ccaaattcac aaaagcgtcc ttaccttgag ggtcgtaaaa
    16561 acatcaaagt atatactgca attccacata aaccggatcc ggaaaatggc ggtatggttt
    16621 taaatgcagc atatggcgat ggtcctgaaa tcactaagat agaaggtgtc ggcaatggcg
    16681 gtaatttcca ggaattgaca gatgcatcta ttcaaacaat acttgccaat tcattcatga
    16741 aagaccctac ctataaaggt agaagggcgc ctgttgatgt tatggtttat gacccggttc
    16801 gggtgcctgc agcagacttt gaattgcgtt tggtggattc aacaaacagt aatccgttaa
    16861 atccggatag tacctggtgg gtattaacca acttaacgga aggtgtttct gtgacttccg
    16921 attttcctat caatattgtg aacgaacaag tattgtcaga ttggggactt gcagtaacag
    16981 tacacaatgt gtttgatccg ggtggtcaga cagtgagcgg atcatttgtt gaaaaagaag
    17041 tgaataacgg atttattagc gcaacattat cttatactga tcctaataaa cagtggttga
    17101 caggtgttcc tgataatgat ggtacgttac tcgactggat acgctccggt attgctgatg
    17161 ggactttcgt agatgtattg cctaacccgg atgaaaatca ggtgtttgaa ggcgtgctag
    17221 gcggaacatg gtcgccaaca gaattggttg cctttagtac tgccgaagta ccatatgttc
    17281 cattccgttc tatcacaggt ctgcgtccgc aaattccgat tgaagaaaca attggtgtgg
    17341 atgtggtatt tacatcagat cgcagcaaat ggtcgcgttg tattgttgtt gaaactggtg
    17401 aatgcgaacg ccctactggt gagattaaat tggatatgcg tcagcaacaa tccgtaaatc
    17461 aggatggtgt tgccgaaggt tcagaagttg gttactcatg gttcccgggt tatgcaattg
    17521 atgttgaaac aggaactcgc cttaacttat tctttggtga agacggatgt gccgttcaac
    17581 cagatggccg cggtaaagac atgtgtttca acccatcaga tgtattcttt gatgaaacat
    17641 tcaatcctat ttatggtggt aaacactata tgtatgtttc taaaactcaa tacgatggtt
    17701 gcgaaaacat ccatgattct ttgcaatgga taggtggttc taaacctgaa acttctgttt
    17761 acaaggatat tttcaaaaat attgtatggg tttcaatgcc attcctgaat acgggatata
    17821 caagtgcggt aggttcacca atgttagttc ctactgagac tactattcaa ttacgtgtaa
    17881 cgaaaccata ttctaataaa tttgtaacag gcgaaaacct cggtttcccg ctgtatcaat
    17941 tctcaactgc gaatcttgct ccttcagtaa atgatactga aacggctagt gcagcgcttg
    18001 atttaattag agtggttccg aatccgtact acgcctattc tgcatacgaa acaagttcgt
    18061 tggataatgc agtgaaaatc accaacctgc catctaaatg tactatcagc attttcactc
    18121 ttgatggaac attggtgcgc agatttgaac gcgatgttac ttcagataat acagcaggtg
    18181 gtgaattgaa ttctaagtcg tcgaacttgg atacttcact cgaatgggat ttgaagaacg
    18241 agaaaaacgt gcctatcgcc agtggtatgt atatcatcca catcgatgca ggcgaccttg
    18301 gagaaacaac agtgaaatgg tttggcgtaa tgcgtccgat cgacttagac acattctaaa
    18361 accgaaaaca caacatagca gaaatgaaaa ggatattaat agcactgaca ttatgcatcc
    18421 cggctggtgt gtttgccggt aacccggatc gttcgggtga agccggagca tcagaattgc
    18481 tcatcgatcc atgggctcaa agcagcggat ggtacggact gaatattgct tcggttcgtg
    18541 gtgtggaagc accaaatatc aacgttgccg gtatgacctt catggatggt tcacaattgg
    18601 aattcgaccg caccgaatgg ttggtgggtt ctgatatcag ccttaacact tttggttttg
    18661 ctaccaaagt aggcgatgga gcactaggca ttaccttaac atctatggac ctcggcgaga
    18721 tcgaggttac aactactgat aaccctgaag gaacaggagc agtttacaaa cctcgcttct
    18781 ctaacttcgg aatttcatac gcaaaacgtt ttgcagatta tctcagtggt ggacttactg
    18841 ttcgcgttat ctctgaaagt atctctgacg taaaagcgat aggcgtatca atggatgttg
    18901 gtatccaata tatcagcggt agcgaaacac accctgagca aattaaaatg ggtatcgctt
    18961 tacgcaatat cggaacacca atgaagtatt ccggcgacgg tctcagcttc cgtggaaatg
    19021 tacctgatgg tgattattca atgactcagg aattccgttc agaatcattt gaattacctt
    19081 cactgcttac tatcggtctt tcttatgatt tctatttcgg cacacataac cgcctcaccg
    19141 cagttggaaa ctttacgtca aactcattct ataaagatca gtttggtgcc ggattggaat
    19201 atggtttgaa agtgaaaaat actgaaatgt tcatggttcg cgcaggatat cgttatgaaa
    19261 acggtattac cagcgatgta gatcgcacaa cagcatctac aggcattgct gccggtgtaa
    19321 cagttcaata tccaatagat gatcttggaa aaaacttagt agcacttagc tactcataca
    19381 gaaccgcaca acagtatcaa ggatctcatt gctttggtct gcgtctgagt ttctgataga
    19441 tttcttttat acctttgtgg aaacgtttct taaccggaaa cgtttctttt ttttaaaaat
    19501 acctaagcat atggcagaag tatcatatct gagtaaagaa gggatggata accttaaagc
    19561 ggaaatccag gagctgaaga ccaaaggccg cgcagaaatg gcccgccaga taaaagaggc
    19621 acgcgaaaaa ggcgacctgt ctgagaatgc agaatacgat gctgcaaaag aagcacaagg
    19681 cctacttgaa atgcgtattg caaaattgga ggaactgctc gcaaatgcac gtatactgga
    19741 tgaatctaaa atagatactt ctaaagtatc tgtattcacc actgtaaagc tgcaggatat
    19801 caagttcaaa cgcgaaataa aatatacctt ggtatctgaa aaggaagctg atgtgaaaag
    19861 cggaaaaatt tctgtcactt cgcctatcgg caaaggatta cttggcaaaa aagttggtga
    19921 ggttgttaaa attaccgtac ctgccggagt gctggaattt aagatactcg aaatatcaat
    19981 gtgatatgcc ttccatattt acacgcatca tcaatggcga aataccttgt cataaaatag
    20041 cagaaaacga taaatatttt gcctttttag atattatgcc tttggcaccc ggacatgttt
    20101 tggtggtgcc taaagtggaa gttgactata tctttgaaat ggaagaagat gatcttgcag
    20161 gaattttacc ttttgcccgc aatattgcca tcgccatgga aaaagcgatt ccttgcgaaa
    20221 gaatcgggat ctcagtaatc ggtttggaag ttcctcatgc acatgtgcac ctgcttcctt
    20281 tgcgcacaat ggatgatatc aatttcagcc gacctaaatt aaaaatgtcg caggaagaac
    20341 tcgcctcaat tgccgagaag atcagaagtt atttataatc tattttattt ttccgggatt
    20401 ggcctgaatg aaatcagacc atcctttgta ttgcttcccc gcaacggccg gacgctttga
    20461 tgcattaaca tgacacacag ctactgccaa tgcatctgtt tcatcaaaat atttcggcat
    20521 ggactccagt tgcaacatgc tttgtaacat tttggcaacc tgttctttac tcgcatttcc
    20581 tttccctgtt atcgattgtt tgatgcgacg gggactatat tcaataacct ccagtccgtt
    20641 gcttgcggca gctacaatag cagcgccttg agcacgacca agtttaagca tggattgcac
    20701 attcttccca aaaaacgggg cctcaatagc catcacaaca ggttgatatt ccttaatata
    20761 ggtacttagc ttttcaaaga tgatactcaa ccgcgtgaag tgatcgtcag tcttttttaa
    20821 ctgtatcaca ccattgatga taattttgat tgtattttta tccgcagaca caatactgta
    20881 tcccagaatg tttgttccag ggtctacacc gagaatgatc tttttttctg aagcagcagc
    20941 cattgattat aaaatatgaa aaccacgaat aaaaaaacac tctggtggtc cattgccata
    21001 aaagtagtgg tttttggcat attgctcttt gccttatata aacaggtgta cctcaatgaa
    21061 aatgtacatg ccattcctga gcagtttttg agcatcggcg caggcaaggc tatcggatac
    21121 ggaattctat tagtgatcct tatgattatg aactggggat ttgaagcatg gaaatggaaa
    21181 aaggtaatag ataatatcac acctattaaa ttcattcgcg catttaaagc cgtttggact
    21241 ggagtgacac tcgggttgtt tacgcctaat cgcgtggggg aatttggagg aagaatttta
    21301 tatgtgccaa ggaaattccg cattaagggg gcaattgttt ctttgatagg tagtttcagc
    21361 cagaatctga ttacggtaat tgccggaatc gcagggttac tttggtatgt ttatgctata
    21421 gaaaaaattg acctgttggt gactggcgcc ctcgctttgg tagcggtaat cgctgtcatt
    21481 ttactcctgc tggcgtatta taacctggat gtaatggtca accttttccg caaggcaaag
    21541 tttctgaagc gcatctatcc ctatacaacc gtccttggcg actattccgc aaacgacctc
    21601 tcaaggctac tcctgcttgc ttttctgagg tatagtgtat atgcggcaca gtatttgatt
    21661 ttactcaatg tgttcggggc agaattatcg gtgatgacgg gtttagcggc aattgcagtg
    21721 atttttctgg ctcaaacagt ggtacccagt ttcgcgatag cagacttgtt gaccagattg
    21781 ccggtggcag cattaatact gtcgaaatgt gatgtttcgt tacagatatc actggcagca
    21841 acaacaagca tctggatcct gaatctgatt ataccggcaa tagcaggtta tatttttatt
    21901 ataaggttca acatatttaa aaaccgacaa tcatgattaa accacttttg atcactgcgc
    21961 tctcgtttag cgcgcttgtc ccggggacca acgaagactt ttcgtccggg cgtgtggtaa
    22021 caccttcaga aagttgtgaa ataccacata tggctttcca gggaggtgaa tcgctgacct
    22081 ataaagtgta ttataattgg ggaacactat ggcttagtgg tggtgaagtg tacttcaaac
    22141 ttactgaaga atccttcaaa ggaattccgg tattgcacgc ttcaggtgag gctatcagtt
    22201 acaaatcgtt cgactggttt tttaaagtgc gcgataaggc cgatacttac atgaataaac
    22261 aaacattgca gtcttataaa ttcagtcgcg atgtatatga aggtggatat actttttatc
    22321 gttcctatga ttgggatcgt gaaaaaaata tcatctattc ataccaggat aaccacaaag
    22381 gcaagaaaga aactaaaaca atttataatg cagatccatg cggtgtcgat ttattgagca
    22441 cattttattg gattcgtact attgattata gcggattgaa aaaaggagat aaagtacctg
    22501 taaaaatggc tattgatgat gcacaatatg atatgtacgt gcgctatgat ggcaaagaag
    22561 tgtatgaaac taaactcggt aaattcaatt gtgtgaaatt aaaaccattg ttgcaggaag
    22621 gcaaagtatt taaagaaggt gaaggtatga ctatatgggt taccgatgat caaaacagaa
    22681 ttccggtgcg cattgaatca gacctgcgtg ttggacgcat cacctgcgac cttaaatcgt
    22741 atggtggcaa caaattcccg ttcacttcga aagtgaatta attaccaagg ctgttgtgct
    22801 tatctatata agacctcctt cttgtaggga ggtcttttta tttgtatgcc ttcagcgtta
    22861 caaaaaactt agcttaccac cggaagtggt aacactgaga gctgcctgtg tccctaatcg
    22921 aataggaagg ttgtgttcga tatgccctgc cggaaaatta aaacaaaccg gaatgttcag
    22981 gtgcgcaaca tgctccaata tgatctcttc aatttccatt ccgaagggaa caggattgtc
    23041 tttcatgtcg ctcattccgc caacaattaa tccttttata tgatccagtt ttccagcaag
    23101 cttcatactc atcatcatac gatcgatgtg atatttgtat tcatcaagat cttcaataaa
    23161 taatattttt ccagcagttg aaaatccata gcgcgtacct aacaaactat ataaaatgga
    23221 aagatttccg cctactaaaa ttccggaagc ttcgcctatc ttattatagg atgttgcctc
    23281 tgaggaaatg gtaattgatt cactctttaa aattgcattt atttgtgcga tagcatctga
    23341 ggtgtttgtt ggaaagctgt aaggcataga acaatgcagt gttggcagtc cgagtttgtc
    23401 gttaatatgt gcgtgtaaaa tagtgatatc gctaaatcca catatccatt ttggaaattg
    23461 cgtgaacaat gtaaaatcta atcgatcgat gatcctgatg gtgccgtaac ctcctcgcaa
    23521 acaaaatatt gctttaatgg tattatcgtc gagcatcatt tgcaggtcgg aagcgcgcgc
    23581 tttatccgta ccggcaagct gaccgttttt tgataaggcg tgtttgccaa caacgggaat
    23641 aaatccccat tcgctgattt gcgccatggc tatatcgacc gtttcttgtt caatccaacg
    23701 cgcggttgct accaatccaa tcttatctcc ttgtttgagt atcatactgt tgcgcataga
    23761 atgtgtaatt ttgcgtccaa aattgcctaa atcttgcaag aacctaaaag atatttgatc
    23821 acatccgcac ttccgtatgc caatggtcca ttacatattg gacatgtggc cggtgcgtat
    23881 ctgcctgccg acatctatgt gcgttatctg cgtaaacagg gaaaagacgt ggtttttatc
    23941 tgtggttccg atgaacatgg tgctgccatc accatacagg caaagaaaga aaatacaacg
    24001 cctaaggcaa ttatcgataa gtacaacgcc atcatcagca aagcattcag cgacctgggt
    24061 atcagttttg atatttatca tagaacgagt gcgcctattc atcatgagac ctcgcaggag
    24121 ttttttaaga cactgcacga taaaggggtg tttgaagagc gcgaaagtga gcagtattat
    24181 gacgaggaat ttaaacagtt tcttgccgac agatatatca aaggaacctg tcctgtatgt
    24241 gcaaatccaa atgcttatgg cgatcagtgc gagaaatgcg gaacgtcact ttcaccaacc
    24301 gacttgatag aacccgtttc tacgcttagc aataagccgc cggttaagcg aaaaaccacc
    24361 cattggtatc tgccacttaa caaatttcag gattggctgc aaagttggat tgtggaagga
    24421 gagggtagaa ctgaagaatg gaaaaagaat gtactaggtc aatgtgcaag ctggttgaaa
    24481 gagggcttac acccgagggc aattacgcgc gaccttgatt ggggtgtgaa ggtgccattg
    24541 ccaaattctg aaggtaaagt gctttatgtt tggctggatg ctccgattgg ttatatcagc
    24601 gctaccaagc aatgggcatt gaatacggga aaggactggg agccgtattg gaaaagtgcc
    24661 gacaccaaat tattgcattt cataggcaag gataatattg tattccattg tctcattttc
    24721 ccttgtattc ttaaaagtca tggcgaatac atacttccta caaatgtacc cgccaatgaa
    24781 ttcatgaatc tcgagggcga taaaatgtcg acttcccgaa actggaatgt gcaggtgcat
    24841 gaatttcttg aggagtttcc ggggaaggaa gacgtgctta gatatgtgct gtgtgccaat
    24901 atgcctgaga caaaagatag tgagttcaca tggaaggatt atcaggcaag gaataacaat
    24961 gagctggttg ccattttcgg aaactatgcc aatagagtga tggtgctgac agaaaagtat
    25021 tatggctcaa aagtgccggc tcaggtggat tctgtagagc aagatgctta tgtagtggct
    25081 taccgtgctc aagtagctca actttttaat gattactgca caggcatcga atcttacagg
    25141 ttcagagatg ctttgatggc tttcatggat attgttcgcc ttggcaacaa gctgcttacc
    25201 gactttgaac catggaaact atataagaca gatccaatcc gaacagaagg cgttttgcgc
    25261 atttgcctgg agagtttgta tgatatggcc accttatgtg agcctttgct tcctttcagt
    25321 gccggaaggc tgaacgctta tttcaatcta cccgcagctc ccaaaatggc ggttggcggg
    25381 gtgaatttaa ccagcggcgc cggattgggt tcgccattct atttgttcga aaagattgaa
    25441 gatcaggtca tccagtctaa actagacgcc ctggagtcta tacggaggtc gcgggaagca
    25501 gtcacagcag cggaaactac tattattgcg gccaatccga tcaaggaagc cattagctat
    25561 gacgactttt ctaagcttga tttgcgttca ggtgtggttt taagtgccga gcgtattgcg
    25621 ggagctgaca agctgctgaa acttacggta gacttaggtt tcgaggtgag aaccatcgtt
    25681 tcgggcatag ctttacattt cagtccggaa gcgcttacag gcaaaaaagt ggtggtactg
    25741 gccaatctgc tcccccgcaa gatgagggga gtggagagca atggcatgat actgatggct
    25801 gaaggtaagg atggtgcgct ccatttcgtt gccgcggaag gaaatgccgg tcccggggac
    25861 tccataagtt aaatatttac atattagcga aatcacgcaa ctaagacgct ttactgttcg
    25921 tcttggaggg gttgttcatt actgaaaccc ttgatatgaa aggaaaaatt gtttttcgtc
    25981 gtataccgac cgattgtaag aataccctta tctttgcacg aaattttaaa aactctatga
    26041 agaagttttc ttcaataacc ttacttctat tagttgtagg cttcacccaa accctgtatg
    26101 cacagagcac tcccatagtt ccgggagtat cgtcttcgac attagaatca cttggatttg
    26161 actctgccga gattgatgcc atcatgagca caacaggtag ttcaacttct actgctgttg
    26221 aagcagcggc acagattcag gatgctcagc aaagtgcagt tcaggctgct aacaatcagc
    26281 tgaatacttt agcacccaac acggcaacat tgcctccacc ggtagttgag caggtgattg
    26341 agcaggcgat atcaaatgat actccttcaa gcgctccggc aggaatttgg ggtcaggata
    26401 ttttccgcaa tggctctatc gatctatttg ataaggttcc gaatgcaaaa gttagcgata
    26461 actatatcat tggcgccggt gatcagataa atgtttctat ttacggttgg gcttactata
    26521 atggtaatct tactgtaagt gaagatggtt acatcaccac cgttgaaacg ggtcgcattt
    26581 atttgaaggg acttactttc agtgcagcac gtcagcttgt tcgtcagcgt tttgcatctt
    26641 ctttcgacct tgtgcattcg acattcgata tgacattaac atattcaaag acgataaaag
    26701 ttagtattgt cggagaagta gttaaaccgg gcacatataa tatttcatct gttaatactg
    26761 cattcaatgc attagttgca gcaggaggtg ttactgatat cggttcagtt cgtaacattc
    26821 aggtgaaaag aaatggtaag gttattaaga cactcgatgt ttatcaattt ttgatgaatc
    26881 cgggttccac tgatgacact tatcttgaag caaatgatta tatcattgtg aatggcattg
    26941 gtcgcgtagt ggatgtgcag ggagaaatta atcgcccttt gaaatatgaa ttagtaaaag
    27001 gtgagaactt aaacgaactc attgcatatg caggcggtct tcgttcaact gcttataagc
    27061 gtaacgtaac tattttccgt tacatcaata acgagaatgt ggtattggat ttcaacctcg
    27121 acagtcttga gcgcgagcga gtgaatttcc cattgttgga tggagataaa atcacttttg
    27181 caagaattcc agaggtagtt gagaatattg ttactattca aggtagttgt cgtttccccg
    27241 gaaactatca gcttacagaa gatatgcgta tttctgatct catagcgaaa gctaaaggtt
    27301 tgaattacga tgcatatact gatcgtgcat atttaattcg caagaatgac aaattgaacg
    27361 atgtgtatat tccattcgat ttgcaggaag ttatagataa tccgaattct gcattcaact
    27421 tcaaattaac aaaatttgat gtgattgata tcttcagcaa acaggaattc cgtgaaacat
    27481 tcaacgtttc tatttcagga tccgtgaagg ttccaggtat attcccatac tatgaaaaaa
    27541 tgacactgaa agatctgttg tattattcag gaggtttgaa ggtggaagcg gctaatagcc
    27601 gaattgagat ttctcgtatt gtgaatttca atgaagcgaa ttctgacaat gaacctacac
    27661 gtgtagttat tcaatctatc agcattggca agcaacttga attggaagat ggtgcaaaca
    27721 ctttccaatt gcaaccatac gatcaggtat ttgttcgcaa tacacctgaa ttcgaatatc
    27781 agaaaaatgt tacagtactt ggcgaggtta aattcccggg tgtatataca cttacatcac
    27841 gcaccgaaac acttgctgat gtgatagaac gcgcaggcgg acttacacag tgggcatatg
    27901 ctgaaggtgc aacaatgaac agaaatgatg ttgcttctac actggtattc cttcataaag
    27961 cattggaaaa tcctgaatca aaatataact atgtattacg tgaaggagat gtgattactg
    28021 ttcctaaagc aggtgatctg gtagctttga aaggtgaaat taaatatccg tttgttggcg
    28081 atccgggcac tgtaattgtt ccatttgaaa aaggtcgttc tgctcgtttc tttatcaaga
    28141 aatatggtaa aaactttgat gatgatgcta agcgcagtga aacctatatc gttgaaccga
    28201 atggttatgt gcgcagaacg cataatctct tattcataca tttttaccct cgagtaaagg
    28261 taaaaggttg tcaggtagtt gttccacaga aacctgaaga aaagccggat gcacctgaag
    28321 cgccatcagc accaagggag ccatttgact ggaatacctt tgccactaca cttagtgcag
    28381 gtattctgag ctttgcaact atatatgtat tgatcttaaa agtaccatca taactgtaat
    28441 tgctcagata agcaaccaca actatgcaat acgatcgtcc gtataatgat gaaataaaca
    28501 ttggtgacct ctttactaaa tttggagagt atcgcaggta ccttgtaaaa aaatggtggg
    28561 tgattcttat agccgcaatt ttgttggcaa taggattgcg attcttcgct gtttggaaaa
    28621 aggaatatta tatttctcat gccgatttcg cggttaaagg aactgaaggt tcgtcaacct
    28681 catcactggc atctttggcg agctcatttg gtattggtat cacaaccggt acagagttta
    28741 ccaatgagtt gttccttgga attcttcaat cacgtaccct tgttaaggag gcgttgatgg
    28801 agaagcgcac cttgacagta aagaagggtg ttgcacctcg tgaggactat ttggccaatt
    28861 tctatgtaga gatgtatcca cgatgggcta aaaagaaaaa ggttaaaaac ttcaaactgg
    28921 aacatggctc attagattca ttgaccgttt atgaggatag ctgtcttact gttatttatg
    28981 atgagattat tgaaaatgat atctcaacag aattcagtga tgaccttggt atgaatcaaa
    29041 tggaagtgtc ttcccttagt agagattttt cttactatct ggcagattac ctcgcttatg
    29101 caggttctaa ttactatatc aatgcacaag tagtcaacga gcaaaaaact gttaacctga
    29161 tgtccgaaaa agcggactca ctcagagcgt tacttgatgg gaaagaggaa ttgttggcaa
    29221 ggattcagga tggaagtgct tatcaggtta aactttccgg atatgtgaac cagaacaggt
    29281 tgattcgtga aataggactt atcaccactg aatattctac tacttacgca cagttgcaat
    29341 tagcgctttt cgatcttcaa aacaagacac cgcttgtgga tatgattgat caaccgaagt
    29401 ttgctaccat taaagaaaaa gagcaaacta cgatgttcat gatcattgga tttattttgg
    29461 gaactatact tacaagcatc gtattagctt tcagaaagta tattcgtgat agcgtagaag
    29521 aagctcgtca gaagcaactt gttattgaaa aataccataa agaacaggaa agtcagtctt
    29581 taatagacac tgacagtaac aaaatttgat agtgagttaa aattcggaac ctgccatgat
    29641 ttttaactca ttacaattcc tgattttttt accggtagcc atcttcctgt acttctctat
    29701 tccgtacaag tggcgttggt tatttctgtt attttcaagt tactactttt atatgagctg
    29761 gaaggccgag tatgccttcc tgattgtatt ctcaacaaca gttgattatt taacagccaa
    29821 cgcaatggcg aagcaaaccg ataaacggaa gcggcgacca tatatgttga tcagtttgtt
    29881 ttcaaatctg ggtttattgt tctcatttaa gtatttcaac ttctttaatg acgctactcg
    29941 ggcagtattc gattcgttcg acattttcta taatgttcct gaattcaact tgtttttacc
    30001 ggtaggtatt tcattttata cctttcaggc actgagttat accattgatg tttatcgcgg
    30061 cgatgtaaaa gctgagaaac actttggata ttttgcttta tttatttctt attggcctca
    30121 gcttgttgcc ggccctattg aacgtccggg agatttacag ccccaattga aaagtaatca
    30181 tgatttcgat tacgaccgcg ttaaaaaagg tctcaccagg attttatacg gatttttcaa
    30241 gaaagttgtc attgccgaca gattgggaat atttgttcag gcggtgtatg gtcagtcgaa
    30301 tggaatggga ggttggaatt atccttcgtt tgaacatggc ggatggccgg tagttatcgc
    30361 aacatggtta tttgctattc aggtgtattg cgacttcagt gggtattgcg atattgctat
    30421 aggatctgcg aaaatcatgg ggcataatct gaccgacaac ttccgaacgc catacttctc
    30481 gaaatcgata cgagaattct gggagcgatg gcatattaca ttaacggtct gggtgcgaga
    30541 ttatctgtat attcctttag gaggaagcag agtctccttt ggaagaatgt tattcaataa
    30601 ctggtttaca ttaaccgtga tgggtttctg gcatggggct aacctgacat atgtaatgtt
    30661 cggttttatc catggttttt atattgtagt aagtagggtt agagataaat atatgccaaa
    30721 aatggtactg gcaaagtgga taccgaatat gccaattctg gtaaatgttt tgttgatgat
    30781 ctggattttt aatctaacct gtattccgga tatatttttt agatcgaatt ctataagtga
    30841 tgcgtggcat attatcggcc agatgttcaa ggcaagtacc atgcatgaat ttatttataa
    30901 cagtgcacca aaaggagcag ttcctagtgt aattgaattt tggatcagtc tggtatttat
    30961 tgctatgctg ttattcatcg attataattt gtatattcgt aaaagtgcga ccattgaaga
    31021 tcaggtggct attcgccctt ggtatacgcg ctggagtttt tatgtaatat tcctggtaat
    31081 agttgcatgt tttgcagtta ccactaaatc agcatttatc tatttcgcat tctaaaacat
    31141 gaaaaaacta attctcagaa tcggattgtt gttggtaatc gttttcgcga tctgccagct
    31201 tatactgatg agtatgcctt tttcatgggg taacacgcgt ttgaatacga agtatactac
    31261 atattctgag aatcctgaac aatacaatac attattaata ggagctagta ctacataccg
    31321 tcacattgat ccaaaacaat ttgatgctgc agtgaatgcg cagtatcctg atttacatat
    31381 aaaatctttc aacttcggta tacctgcaaa tcgcacgcct caaagtatgt atatgcttga
    31441 aaatttgttg aaggaaggca ataccgatca tataaaatat gttgtgatcg acttgtcgga
    31501 attgacaaag atgggtgccg ataacttgca taaaaaggag atgttgtact ggtacaactg
    31561 ggggaatatc ggtgatgtga tgaaggcctc atatgagtct gaaaagggca cagcaaataa
    31621 attcggggtg ccattcttac atgctttcag ctttggtgaa aaatcttttt tggttggtat
    31681 ggggccgtca gttattatgc agcatgccgg tttgaatatt gagcctttat ctgttggtcc
    31741 tgataaaaac ggcttctatt ctcttgatca ggaaatgcat gatgatcctg aaggtgatct
    31801 ggcaattcga tataatgatt tgaggacaca agacactatt gacttccgca cacagcgctg
    31861 tcagatgctg tatgataaat tcaaagatgc aaagaagaat cctaacaaaa ccatggaagc
    31921 ggatttgttg aagatcatca aagattgtga aaagcaggat atcagcgtag tgataatgct
    31981 ttctcaacgc cttggcgacc gctatgaata tttgattcct ttgtataatc gcattcctga
    32041 aaaaaataga attggatttc aaaatccatc tgcatatccc atgttcaatg agcgagagaa
    32101 cttatttgat ttggcgcact taaattctag cggcgctaaa ctatttacgg atattttcgc
    32161 tgatcaatgg attgatcgct tgatcatgca aggtaaaata gctccaagac ctgttgcacc
    32221 gcttaattct cttcaacaaa ccaattccgg taccggcgat gcgttatgat cagaaaaata
    32281 acaaagaaat tccgtcgtaa gctcaaggat caggatttta aggagatctt tagtaaaggt
    32341 atttcaggca ctgccataag tcttgttggt agattttcag gttatttact tttgatggta
    32401 attggctggg tgtatggtca ggaagctatg ggcgtttata ctgttggtct gagcatcctt
    32461 agcattgttg gaattttcgg aagatttggg gtagatatgg ctatgtcgcg ctttgtagca
    32521 cagtatggcg ggtcagggcg ttgggatttg atcagacat
//
LOCUS       DAOQOU010000016        32441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_605_length_32441_cov_6.316526, whole genome shotgun sequence.
ACCESSION   DAOQOU010000016 DAOQOU010000000
VERSION     DAOQOU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 32441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32441
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_32441_cov_6.316526"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLM90_02610"
     CDS             <1..782
                     /locus_tag="PLM90_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HMZ88288.1"
                     /translation="PTFHGDNVKEDKNIAWEMLELALGEKDLYTWIGAVETRKGTSKQ
                     STYTSCLRLQKEIAQLKRLLTNRLPDKPYYELDIDEKTLSNLRTSLAARPFASERFSK
                     HGEIFCALGSKDKNPYKTNFGASLGMGPKSGLSAGYYLQIQPGNASFAAAGAYMPELP
                     VLAAMRQEIDYNLKDFEKILKAASFKKYYEGLDEIEVLKTTPKGYSPDNQALPYLRHK
                     SYVVSRAFSDKEVTDPSFLKEVVNTLKSAHPLVQFLNKAGS"
     gene            835..2697
                     /locus_tag="PLM90_02615"
     CDS             835..2697
                     /locus_tag="PLM90_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ88289.1"
                     /translation="MKKCTLLLLLAGILSTGYSQSITAFPDTTICSADSVMLYAVVDG
                     SYGTESYTYAEIPYAPEPIGGTVHNMVDDTHTGPFSIGFDFCFFGQVYTQFYIASNGW
                     ISFLTPTGGMDVNWTPDGPIPFGAGVTPKPAIFGPWTDWHSGLCTNCIFHEVVGVAPN
                     RKLIVTYEDVPLFSCTAFEGTFQFVLHETTNVIENHLTHVDVCPTWDLGVAVQGIINE
                     DGTEAFSVAGRNATDWFADDESWRWIPDAITWYETATGIVVGTGDSIYVNPDVTTTYT
                     AEITLCDGTIATDEVTVTVSTPYEVTLDVQQIACFGATNAWIDVDVTGNTNPITYTWS
                     TGSTIDSIYDLGAGTYTITIQEEDGCAWEEDVVITEPPLLTLDTVLTVDVTCFGGSDG
                     VVNLNPGGGVLPYQFSFDGINWQTDSNFISLEAGTYTFTVRDAFGCTTSFTGVVISQP
                     PQIMVDAGPDQSIAYGQSVVLLGTTGAPVVTAISWSPVDYLSCIDCLQPTCTPTFNST
                     YVLTVTDANGCQASDSVNIWVDIDFVVPNAFSPNDDGLNDVFNISTDLLISYDIRIYD
                     RWGAEVFHSTDINFGWDGKLGGLPQEIGTYVYVIKSTTTLNTPITKSGTITLLR"
     gene            3030..4637
                     /locus_tag="PLM90_02620"
     CDS             3030..4637
                     /locus_tag="PLM90_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88290.1"
                     /translation="MISRKLTIAIVLLYSGLTFAQTSSFPPKPTDQWQIGLNLGLPII
                     QGDLRAEPFGGNHAPAFGVGLNVRKSLGYIVSLRLHGMYAQTYGMDYVQGSYAYNKTL
                     NGAFYDAINVDGQPAENYYSTGANYTNNVWVPNYKTQFGHGSLDFIVNLNNLNFHQHD
                     PRFYAYAYAGVGAMAYNTKYDALDADGNPYPFQTILEGFGSQNTSQKERLDALHAALD
                     GEYETQAEVANPAARGADEVRATWVRNPSISGGFGVSMRIGSKMELGLDQRATHYFDD
                     LADGQRWQYDGTYSPHYDLLTYTSLNFGYNLGKAESIDPMWMVNPLAVLYDQMAEIDP
                     DNMLKDTDNDGVIDRLDKEPNTPEGTPVDTHGVSLDSDKDGCPDSEDPEPFSSPVLPM
                     ENCQNVHLTQEWVESYVDQRLSKYTPGASTDWILPYIFFDLGKSNIRPDAVPALNQVA
                     DLMKRYDKLSVEVVGHTDTRASEEFNMKLSEDRARNAINYLTSKGVPESRLKIVYKGE
                     TENLIKDANEEAEHQMNRRVEFHIIKN"
     gene            4762..5547
                     /locus_tag="PLM90_02625"
     CDS             4762..5547
                     /locus_tag="PLM90_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88291.1"
                     /translation="MLLRCKILALVFVVYLLNPVHAQNNDNESKAHKHRRLFHRQVIC
                     VYYPNPLSYLYDFPAYTSELDTMDSDMDGVVNRYDKEPNTPALSPVDIHGASLDSDRD
                     GCIDAEDPEPYSDVFDSMVDCHNVYKEIPLTIESCAHLRLDNQLSGADDWILPFIFFD
                     AHKADIRPDAEAALTMIAEIMIRYPMLSIRAYGFSAGGVEKDSSALLVERRIISSVNY
                     LIELGVPEDRISVVKYRKPKQIFYDGDIVEGFPRIVEFWIESN"
     gene            5607..6377
                     /locus_tag="PLM90_02630"
     CDS             5607..6377
                     /locus_tag="PLM90_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88292.1"
                     /translation="MKLTLLWILICLTHLCYAKNALSDSVKFNSYRVTVDINENQVIF
                     SFFPLNYIICQVDFDPDSLLLDDDNDGVYNTIDLEPNSDDWAADIHGVTLDSDHDGCP
                     DHLDEEPYSSPVLPMENCVNVSIGCIWDVGVSGREYVEHAYTTWCLPYIFFDPAEAVI
                     RPDAVPSLNQIADILIRYDKLTLRVVGYSDSTSDEGVNVKLSADRAGEVVNYLHSKGV
                     PESRLIVVYKGETSDHIKNEKSVVEHQLYRRVELYFER"
     gene            complement(6360..7706)
                     /locus_tag="PLM90_02635"
     CDS             complement(6360..7706)
                     /locus_tag="PLM90_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88293.1"
                     /translation="MENRTTRTWQIAAYVLYGFLCTILLYFTSRENFIQLIAIYAGMF
                     ALYFLLARSSERTNYLGEIIIAGILLRLIATFALPNLTDDHFRFLWDGQLVTHGFNPY
                     ASIPEQVRAEGYFGISTLDDHLFNSLNSAQYISIYPPIAQAIYAIGALIGGSNWDIQI
                     LVIKIILLAFECGTIFLLPKVLKQLHINVGHAAWYILNPLIIVELSGNMHLESTMVFF
                     SLLSIYYFNKNNLWLSAISFGLAIATKLWPLMLMPLLFRQIGFKQTMRYSVIAGVTAI
                     IFLLPMLVEFSKITGSFNLYFQQFEFNASIFYIGKYFFDKDVNYEAFSNMRGSLPFIA
                     AFLILVFSAVYKKQYWYAGMLIAFSIYLLLATTVHPWYLAPLILLSVLSGLRYPILWS
                     LLIYLSYYTYITPAYIENMTFVWIEYVMVIGFAAYELIHSPMIESKIITSRLPFKI"
     gene            complement(7717..9192)
                     /locus_tag="PLM90_02640"
     CDS             complement(7717..9192)
                     /locus_tag="PLM90_02640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88294.1"
                     /translation="MSITIIVIYTIAITLLFFYCIMQLSLAIHYIRYKRRMEAEEATF
                     KGPESEYPMVTVQLPVYNELYVVERLIDAVAAFDYPKDKLEIQLLDDSTDESFEIARK
                     KVDFYKQQGINIVQVKRPIRKGFKAGALEYGLQTAKGEYVAIFDADFVPYPDFLKKTI
                     PYFLTDEKIGVVQARWEHINKDYSILTKMQAFALDMHFTIEQQGRNAAGYFINFNGTA
                     GIWRRTAIDDAGGWNSDTLTEDLDLSYRAQLKGWKFKYVEDVVAPAELPTDMNALKSQ
                     QFRWNKGGAETARKIGRRVMGAELPIRIKLHAIAHLFNTTNYIFILITAILSVPLLFI
                     KNHYIDFNYFKYASIFLMGSIAIAYAYYISTLQREKTVGQTVKVYISRFPVFLAITMG
                     MSLHNAWAVFRGWLGQQTAFVRTPKFAINNSGDIWKKKKYVASRVSPITYLEGIFSLY
                     FLSGIIMGIYYQDYGLLPFHIMAFMGFALIFYFSFKHARSN"
     gene            complement(9207..9899)
                     /locus_tag="PLM90_02645"
     CDS             complement(9207..9899)
                     /locus_tag="PLM90_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ88295.1"
                     /translation="MAKINVIIPVFNESGSIGHVLNDIPAGLVEEVIVVNNGSTDNTA
                     EIARSKGATVLLEARRGYGSACLKGIAHIRRKPVEQQPDIVVFIDGDYSDYPEQMPDL
                     IRPITEQGFEMVIGSREKGTRIKGSMAPQQIFGNWLATSMILWLFGARFSDLGPFRAI
                     TWKALESLEMRDTNYGWTVEMQVKAAKKKLRFTEVPVNYRPRIGVSKVTGTVKGTIMA
                     GYKIITTILKYY"
     gene            complement(9975..11348)
                     /locus_tag="PLM90_02650"
     CDS             complement(9975..11348)
                     /locus_tag="PLM90_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ88296.1"
                     /translation="MADQQIDMSLSGSSSANFNGLQKTGIAITSIGILFFFIAWFGVG
                     NQQPLICLLITLAGFIIGGITYSFGTYGHLPKGIKNNRAFFTSISSRGTLGWISGIVI
                     TAFYIALYWRDYWKSVLHTDVFAGLVSMFDPLSQWMRKGPADQWFVYGSLYTLAVFVM
                     GIKFILKYRHNRYQVIRTISVMFFQFGFAWIIPAVMKALYNFEPYYSYFWPLDYDAFF
                     PNSINYFKDQGNLGVFFLFWGAVMAFIATPILTYFFGKRWYCSWVCGCGGLAETAGDP
                     FRHLSDKSLKAWKIERITIYSVLGAITLITAALLIDNSMHNQLLGSSAKWIAKGYSFA
                     IGMVFSGVIGVGFYPLMGSRVWCRFGCPMAAYMGIIQRFKSRFRITTNGAQCISCGNC
                     STYCEMGIDVRAYAQRGQNIVRASCVGCGVCAAVCPRGVLKLETGPENPSRTEVLFTS
                     DDLKILS"
     gene            complement(11354..12691)
                     /locus_tag="PLM90_02655"
     CDS             complement(11354..12691)
                     /locus_tag="PLM90_02655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HMZ88297.1"
                     /translation="MHHVIIGNGITGITAARTLRKLSKDHITVISGETDLFFSRTALM
                     YIYMGHMRFKDTQPYPKDFFEKNNISLKKAWVSKIDSAKKTLHFDSGDHMQYDNLLIA
                     TGSKSNRFGWPGQDLKGVQGLYSYQDLLELEENTRNGINRAVIVGGGLIGIEMAEMLH
                     ARHYPVTLLVRESNYWSNILPAQESALITNHIQDHGFDLRLNTTLKEIKGDANGRVTG
                     IITDSGEEIPCQVVGLTAGVSPNISVLQGTEIKTDRGVLINKRMQTSVEGVFAAGDCA
                     QFTETLPDRRPVEQVWYTGKIQGECAAKNMLGINAEYDPGYWFNSAKFLDIEYQTYGT
                     VLSKLRDGESEFYWEHNDRQKCVHIVFNSSDHKFIGINTFGIRMRHKLFNTWLQESRS
                     VEYVMAHLRAAQFDPEFYSRYEENIIDAWHAQFPDHRITQADEKTIRSLIFNA"
     gene            complement(12806..13591)
                     /locus_tag="PLM90_02660"
     CDS             complement(12806..13591)
                     /locus_tag="PLM90_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HMZ88298.1"
                     /translation="MKHNNLIYRTLIGMMLAVVLLPACRTIDGIDSNSKPVSHEQWSA
                     LLDKHVDDNGFVDYKGFILDSTALNDYLQLLEKGYPNEKNWTREEILAYWINAYNAYT
                     VQLIIRNYPIESIKDIKPGVAFINSVWDVQFINIEGQELDLNNIEHNILRKMEEPRIH
                     FAINCASYSCPKLRNSAYTAENLNTQLDLAARDFINDPSRNNIKNGSAQLSSIFNWFT
                     GDFTKNGSLKDFINQYSNNKIAADTPIEFMEYNWKLNEQNGGK"
     gene            complement(13691..16156)
                     /locus_tag="PLM90_02665"
     CDS             complement(13691..16156)
                     /locus_tag="PLM90_02665"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ88299.1"
                     /translation="MQKTFLYATAIALCGWLINTSAIIAQTAVRPVTQMVRDAHTAKI
                     NFTPAQTLDRVSAPAYRSKAEAFANNITYARLDGDAIHQLFEEKQAAISLTVPYKEGQ
                     NLTLELVQVNLFADGFKVTTSESNGAAVEYTPGVYYQGIVQGDDNSIAAISIFENEMI
                     GMVSTSSEGNINIGKYGDGDWQDYLVFSDRDIIVQPENAGCATVDDESYLDTFHQIME
                     NGGGERITKCPKIYFECDYQLYQNKGSVTNTTNYITGVYNNAAIIYTNETVPTQISEI
                     FVWTSADGYATASSYDALNSFKAFRTTFTGDIAHLVTLDPGGLGGIAATINGLCNSNK
                     YCYSDIDATYSAFPTYSWTVMVVTHEMGHLFGSYHTHNCSAWVGGALDNCYTTEGGCP
                     PGPAPTSGGTIMSYCHLTGYGINLSNGFGTQPGNAIRNTITAAACITSCGGVPTYCTS
                     VGSSTADEWIQTISMAGTTNNSGNNSGYADFTATNINLTPGGTASFTLTPGYTGTVYP
                     EYFSIWIDYNKDLDFADAGENVYNSGAVTSAVSGSFSVAAGMTGTTRMRISMKYNALA
                     GPCEVFSYGEVEDYTASFVPVVTYCTSAGTSAASRYIDYVKIGTITRASGNDGGYYNG
                     TAMSANVIKGSSYTLTYSAGFSGTIYQMYWRGWIDFNRDGDFADAGEQVFQKKATSAG
                     NLNKTITIPTTAGTGTTRMRISCKYGGYPTSCETFANGEVEDYTLNIMPGLPSPEENP
                     YYLNIYPNPSEGVFQMEFLNAFNGGITMEVFDMTGNRIRQEVIESDNGWIVLDISDAA
                     PGMYIVKTTLANGTTESKQIIKQ"
     gene            complement(16363..17496)
                     /locus_tag="PLM90_02670"
     CDS             complement(16363..17496)
                     /locus_tag="PLM90_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMZ88300.1"
                     /translation="MENNNWSNNSVFKTTLIVILTLAGLFGVVWLLMQITSILFYFII
                     AAIISIIGKPIADLLDKLHIGKFKMPSSLSALLTIGVLYGILFGIISIFTPLVIQQTN
                     TISHIDTQKVADGLQQQVQVLDSMLVHVQDPTQPAKSVELILNESLREAINATSLTHI
                     FNNILGGLGNVMVALFSITFITFFFLRGERLFYNIIMVLTPTKLEGNGRRILHSSKKY
                     LTRYFVGLIIQSTVVFILIAVGLLLLGFGANSWLIALFAGIINIIPYIGPIIGMLFGM
                     LIGITTNLSVDPNFDIIPMLIQLLILFQGVQLIDNFVSQPIIYSNSIKAHPLEVFFVV
                     LVFGSLAGVPGMIAAMPVYAFFRIIAKEFFSEWKVVRKMTQDI"
     gene            complement(17589..18563)
                     /gene="arsM"
                     /locus_tag="PLM90_02675"
     CDS             complement(17589..18563)
                     /gene="arsM"
                     /locus_tag="PLM90_02675"
                     /inference="COORDINATES: protein motif:HMM:NF038099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis arsenite
                     methyltransferase ArsM"
                     /protein_id="HMZ88301.1"
                     /translation="MSTTYLETTRNVYKEAAENPQKGLCCTTTPVWQLPGLVIPQKML
                     EMNYGCGSTVNPRDLVNDPTILYVGVGGGMELLQFAYFSRRKGGVIGVDVVEEMLQAC
                     HENLLEAEKLNPWFKREFVDLRKGDAFNLPVEDNSIDIAAQNCLFNIFRDEDLEKALK
                     EMYRTMKRHARLVMSDPTCEQEIPENLRNDEELRALCLSGSLPLQEYINRITNIGFGT
                     IEVRARRPYRILDPKHYATNELLYIESAEVCAIKDPMPADGPCVFTGKTAIYFGDEEY
                     FDDHKGHVMTPNQPLAVCDKTAGNLAALNRDDIFISGSTWFYDGGGCC"
     gene            complement(18586..19668)
                     /locus_tag="PLM90_02680"
     CDS             complement(18586..19668)
                     /locus_tag="PLM90_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMZ88302.1"
                     /translation="MIAIIILLAALFLAYSNGANDNFKGVSTLFGSGTTNFKTALRWG
                     TITTLAGCLFAMYIAQGLMKNFSGKGLIPDDTLQDPNLAIAVAIGAAATVFLATRIGI
                     PVSTTHAIVGALTGAGFIAVGSELNVNQLGETFLKPLILSPFIACGASIILYLLFRQI
                     RTWLRIEKDTTLEIQEPHFAQNSISASTISLTQQPKESYTGHIVGISAQKILDSLHFI
                     SAGAVGFARGMNDAPKIAGLLLLVHFLNVPSMIIIVAAVMAAGGLLNAKKVAETMSRK
                     ITYMNPGQGFTSNLVTAVLVWTASMSGLPVSTTHVSVGGIFGIGVVNRKADYKMIGKI
                     VGSWVLTLPIAAVLAATTFYILQQFN"
     gene            complement(19665..20276)
                     /locus_tag="PLM90_02685"
     CDS             complement(19665..20276)
                     /locus_tag="PLM90_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HMZ88303.1"
                     /translation="MTQDALIIMIKNPVKGKVKTRLAADIGDDAALAIYMQLLEHTKQ
                     VTEMIPQDKFLFYDHVVERNDIFPNSTYKKYTQCNGDLGVRMDYAFSIPFKNEYKKVV
                     MIGSDCYALTAEHLAKAFEALDHHDFVIGPAHDGGYYLIGMKKWNRWVLENKSWSEHK
                     LFQETQADILSRNGTLHLLEPLSDIDELKDIQAISLFKSLIQA"
     gene            complement(20299..21357)
                     /gene="arsS"
                     /locus_tag="PLM90_02690"
     CDS             complement(20299..21357)
                     /gene="arsS"
                     /locus_tag="PLM90_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671343.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HMZ88304.1"
                     /translation="MKTSKSLHAQHHKLADTNYQLKVLETHEVAGADFERFDDKLKAI
                     GLLPLKPTKLEIFQVNVGYMCNQTCAHCHVDAGPDRKEIMTRETMQQCLDIIAKTDIS
                     TVDLTGGAPEMNPDFRWFVEEITKLGRKTMVRCNLTIIMANKKYHDLPQFFAEHKVEV
                     VSSLPFYNKSRTDAQRGDGVFEDSIKALQMLNEVGYGKEGTGLILNLVYNPGGAFLPG
                     SQFSLEQQFKRELKRNFDIEFNNLFCITNMPISRFLDYLIESGNYESYMQKLIDAFNP
                     VAAAGVMCRNTVSIGWDGFIYDCDFNQMLGLHVNSGAPNHISKWDTYALQSRNIVLNQ
                     HCYGCTAGSGSSCGGAVA"
     gene            complement(21530..21868)
                     /locus_tag="PLM90_02695"
     CDS             complement(21530..21868)
                     /locus_tag="PLM90_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HMZ88305.1"
                     /translation="MERSTYYDPKDLKNFGKISDWDGELGRAFFSYYGKVFEEGALTA
                     REKSLIALAVAHAIQCPYCIDAYTEDTMAKGCDEEQMMEAIHVAAAIRGGASLVHGTQ
                     MMNKFKDLSM"
     gene            complement(21963..22667)
                     /locus_tag="PLM90_02700"
     CDS             complement(21963..22667)
                     /locus_tag="PLM90_02700"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HMZ88306.1"
                     /translation="MDFRHPGHMAFVLFTRTREEEARAKDFSATHSLRTNSLIAGHLI
                     GHAERTARNTGLPCFVISSAQQHGNSFGEKLAHAFEDIYSLGYTQVIAIGNDCPTLTT
                     NDLLKAATALDEHGAVMGPANDGGVYLIGLHRKHFDRLYFEKLPWSSDNLFVSLTEYF
                     SVNGISFATSRALSDIDYPTALQQVLKGTQLSTSLRFILISLLAGLYAVINKDQTNPT
                     LSQPRMYVGLRAPPVF"
     gene            22831..23163
                     /locus_tag="PLM90_02705"
     CDS             22831..23163
                     /locus_tag="PLM90_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ88307.1"
                     /translation="MGISKLEDFTVRDNKVSAYAKALSHPARVAILRLLASRSSCMCG
                     DIVDELPLSQSTVSQHLKELKESGLIKGSILGAKVCYCIDPVAWEAAKSYINGLFDDY
                     SPCIDLLC"
     gene            23268..24029
                     /locus_tag="PLM90_02710"
     CDS             23268..24029
                     /locus_tag="PLM90_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HMZ88308.1"
                     /translation="MKIALFSDIHANLPAMEACFADIDTRRPDAVYCLGDLVGYNIWP
                     NEVIRLIRDRGIPVIFGNYDQGIGLHSDECGCAYKTDIEKEMGKISISFTNSIVGDDE
                     RNYLRSLPAHIRVDHLIQEETMSLLLVHGSPRRVNEYLFEDREEKSMLRIMQQANADI
                     MCFGHTHKPYHRILQDGDRYRHAINIGSVGKPKDGDPRGCYVMLTIDEHTSVHAEDAV
                     QVEFVRFQYDVEKAAHAVEESPLPNAYADMLRKGY"
     gene            24032..24670
                     /locus_tag="PLM90_02715"
     CDS             24032..24670
                     /locus_tag="PLM90_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HMZ88309.1"
                     /translation="MRNYVAEALGTFAILFFGTGAAIVDSITGGTVSHVGISVTFGFT
                     VMSMIYVFGNTSGAHFNPAVTIAFYFSGRFPGKEILPYVIAQFLGAIAGSGLLLFLFP
                     DAATRGETVPSGDVLQAFVLECVLTFFLMLTIIHVAHGSKEQGMFAGLAIGSVVLMAA
                     LFAGPICGASMNPARSLAPALMSGNLQSIWIYLTAPVLGAVGAVLGWKWLRR"
     assembly_gap    24835..24934
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24937..25515)
                     /locus_tag="PLM90_02720"
     CDS             complement(24937..25515)
                     /locus_tag="PLM90_02720"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HMZ88310.1"
                     /translation="MSTTAATIKEIIAYLKEHADPSHAEGMKVTAPGAKKIYGVRSPE
                     MNELLKAIKPGSFELVDALWETGIYEARMLAVKLLEWQSKTDPERGIATVIRFSPEID
                     NWAICDTIGMKSLKKLNKKYPERIMSLSEQFAVSSNMWERRLSMVILWDLCKDKKWHP
                     RIKKIIDMHRGDKEKYIKKGVVWLDKTLSDHA"
     gene            complement(25512..26336)
                     /locus_tag="PLM90_02725"
     CDS             complement(25512..26336)
                     /locus_tag="PLM90_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HMZ88311.1"
                     /translation="MSELLAYRDGQYLNKSDIRIGLDDLGFERGFGVFDYMRQHKGII
                     PFLKDHLERLDHSQSVLKFAEPLPMSLVAEVITRLLEANKLDDGYVKVMITGRMQNGI
                     PVPVLTVYQDVMKAYPASSYSDGIKLIISEYDKPYPEYKTTFYLGSFRELARMQAENA
                     DDVLFYVGELVRECSRCNIFYVKGGLIYTPLEHILLGVTRKHVINCALQNFMVIEAIV
                     TMERLFQADEVFISSTTRNIMPVTRIEDRIIGNGKPGYVTQELMQSFNSYLENYSA"
     gene            26441..27655
                     /locus_tag="PLM90_02730"
     CDS             26441..27655
                     /locus_tag="PLM90_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HMZ88312.1"
                     /translation="MARDISISSYTYELPDDRIAQFPLGDRASSKLLWYKDGKIHDYI
                     FSQLPDLLPENVTLIFNTTKVVCARILFSVEGAQKPIEIFVLEPQSNMTMEQAMLQKG
                     RAVWKCLVGNNKSFRADAIKKEIQWHGEQHVLQVNKPQLSGDVFLVEFIWSESLTFSE
                     MLDAAGQIPLPPYMKRGPEQEDSSRYQTVYANEEGSVAAPTAGLHFTDAVINELRRKN
                     IQIEHVTLHVGAGTFRPVKAATMATHDMHAEEISISKETLKHLVNNPDSLIAVGTTSL
                     RTLESLFWIGWKWKHAEQENVPMVNQWDAYDADVPVEFTFVDALHAIISRMEIFGITT
                     ITTKTQLLIAPGYTIRTAKALITNFHQPNSTLLLLVATFVGADWKLIYNHALENEYRF
                     LSYGDSSLLWRK"
     gene            complement(27672..28049)
                     /locus_tag="PLM90_02735"
     CDS             complement(27672..28049)
                     /locus_tag="PLM90_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ88313.1"
                     /translation="MNLNQVTVTVSDIHRSIHFYQLLGLRLIVHTHDKYARFLCPSGD
                     STFSLHVPDSPLTPTGTSIYFEVDDVDTKINEFIKKGISIDHMPEDKPWLWRESELHD
                     PDGNHIIIYHAGVNRIDPPWRLK"
     gene            complement(28063..28398)
                     /locus_tag="PLM90_02740"
     CDS             complement(28063..28398)
                     /locus_tag="PLM90_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88314.1"
                     /translation="MKSTSLTLLAMMILFGCEKYNPAANTPECVADSIKDFSKDAWCE
                     DIKIEEYLFQGGYVYCYDPGTCGNDMDTKVVDANCNVLGYLGGFSGNDIINGESFTSA
                     QFIRTVWEQ"
     gene            28491..29045
                     /locus_tag="PLM90_02745"
     CDS             28491..29045
                     /locus_tag="PLM90_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HMZ88315.1"
                     /translation="MSISITLQTRCAGVCELCGAEPAVIEITVSPKDESTENQVAICT
                     VCETALANADEKNHWQCLSGSIWSTEPAVQALSYRILYGIRDESWAAEILDSGAADES
                     VINWAMKAYEVADVHQDAYGNVLANGDNVILTQALKIKGTSFSAPKGTQVRSIHLVHD
                     NTEQIEGKINGTMIVILTKFVKKG"
     gene            complement(29158..30699)
                     /locus_tag="PLM90_02750"
     CDS             complement(29158..30699)
                     /locus_tag="PLM90_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ88316.1"
                     /translation="MFRSIQWIAATVIAFTISACALDVPGYLDITPYTYSSVDSTGGE
                     WEPVYVHGADLVSIPTPESVTSDAYLTEIEELKSIANNLTSEQSNAIEYWGNNTTIRW
                     IEITEELIAKYNLAPSPDENGEYGVPDQVFPATYPQFPFAHPPYASRAYAYLSAAIYD
                     ALIATWEYKYTYDRRAPYMVDETISPAYPKTSIPSYPAEDAVIASVAEDVLEFLFPLE
                     TAYISNMAYDCRNSRKWASMNVQSDIDAGDSLGHALADIFIGRAKTDSMKFAQVLQEE
                     YDEMEAAANALWSWPNWENLEVPQRPIGITPKFGHVVPWWVDDITTVRPGPPPAIGSE
                     AYETARQELLDFSKDLTDEQERIAYFWGDGFGTYTPAGHWNTIAKEPIIDHAYNPLRT
                     ARVFAYLNTAMMDAGISCWDTKYYYMYPRPPQADPEIKTLFGLPNFPSYTSGHSTFSA
                     AAATVLSYMFPENEVLYNQYMYEASESRIYSRIHFRFDCEAGLTAGTTIGNLAVDAAK
                     ADGSE"
     gene            complement(30724..32073)
                     /locus_tag="PLM90_02755"
     CDS             complement(30724..32073)
                     /locus_tag="PLM90_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88317.1"
                     /translation="MKLKALTLAVACYSCIMIGHSQVPLAAFIGQESLPADSDPICTI
                     PIYPEVSTNLEGPLGGELVHDFKLYTLEGDSVQLSGVLNDGKPVLLVGCNYTCYVFRG
                     KMDVVNDMQAIYGDQINIFLVYTVEAHPVIDFSPYFGYENVGSDNYAEGILYRQPTTY
                     GERKDIVSDMLDAMDIDVPVLIDGPCNEFWAAYGTNPNPAWLIDPNGTVFDAQKWFNK
                     EPDNMYASIDSMLNLVGTGVFTPEGTFTAEDESEEVYYGIVDNTIAAHITLTNTSDDD
                     VLIDFIRADESIPDGWFTAMCTDLCYSPDIDSTTVFLAPGETEIVRVDFFTDLIPSSG
                     YVEINCTNHYQPDNTYSFTLNAASVAETAINNVDGLSSFLTSPNPSMAGNPVTIQYNN
                     TASSATYTLYNAAGMLIGNGNLQLGSSHVIDIDQPAGIYQLLISEEMPVAMATIVVQ"
BASE COUNT         9144 a         6703 c         6958 g         9536 t
ORIGIN      
        1 ccccaacctt tcacggagac aatgtaaaag aagacaagaa cattgcctgg gaaatgctcg
       61 aactcgcact tggcgaaaag gacttgtata catggattgg cgcagtcgaa acgcgtaaag
      121 gcacaagcaa acaatcgact tacacctctt gccttagact gcaaaaagaa atagctcaac
      181 taaagagatt acttacaaat cggctgccag ataaacctta ttacgaactg gacatcgacg
      241 aaaaaacgct ttcaaatctg agaacgagct tggctgcccg cccattcgcc tctgagcgat
      301 ttagcaaaca tggagaaatc ttttgtgcat taggctcaaa agacaaaaac ccctataaaa
      361 ccaatttcgg cgcatcgttg ggtatggggc ctaaatccgg gctttcagcg ggctactacc
      421 tgcaaataca accgggaaat gcctcctttg ccgctgccgg ggcctatatg cctgaacttc
      481 cggtgttggc tgccatgcgt caggaaatcg actacaacct caaggatttt gaaaaaatac
      541 taaaggccgc atcattcaaa aaatactatg aaggtctcga tgaaatagaa gtgctcaaaa
      601 ctacgcctaa gggctacagc cccgacaatc aggcacttcc ctatttacgg cataagagtt
      661 atgtggtttc gagggcgttt agcgacaaag aagtaacaga cccttcattt ctgaaagaag
      721 tagtgaatac actaaaatct gcacatccct tggtgcaatt tctgaataaa gccggcagct
      781 gagattatta tgtttatatt tatagcctat tagccggtca caagtgtaat caatatgaaa
      841 aaatgcacgc tcctcctcct attggcgggc atactatcaa cgggatattc ccagagtata
      901 actgcctttc cggatacaac tatttgtagc gccgattcgg tgatgttata tgcagttgtt
      961 gatggatcat acggtacaga atcatatacc tatgccgaaa tcccttacgc ccccgagcct
     1021 atcggcggaa ccgtgcataa tatggtagat gatacccata caggcccttt ttcaattggc
     1081 ttcgattttt gttttttcgg acaagtgtat acgcagtttt atattgcctc aaacggttgg
     1141 attagttttc ttacaccaac gggaggaatg gatgtgaact ggactcctga tggccctatt
     1201 ccttttggtg cgggagtaac tccgaaacca gccatattcg gaccgtggac cgactggcat
     1261 tcggggcttt gcacgaattg tatttttcat gaggtagttg gtgttgcccc aaatcgcaag
     1321 cttattgtta catatgaaga tgtgccgctt ttctcatgca ctgcctttga aggaacattt
     1381 cagtttgtat tgcatgaaac cacaaatgtg attgaaaatc acctgacgca tgtagatgtt
     1441 tgtcctacct gggatttagg cgttgccgtg cagggtataa taaatgaaga tggcacagaa
     1501 gccttttcag tagcaggaag aaatgcaaca gactggtttg ccgatgatga aagctggcgc
     1561 tggatccccg atgccattac ctggtatgaa accgccaccg gtattgttgt tggaacaggt
     1621 gatagtattt atgttaatcc cgatgtaaca acaacttaca cggcagaaat tacactgtgc
     1681 gatggcacga tagcaaccga tgaggttacc gtaactgtca gcaccccata tgaggttaca
     1741 ctggatgtgc agcaaattgc ttgctttggt gcaacaaatg cctggattga tgttgatgta
     1801 accggcaaca caaatccaat tacttacacc tggtcaaccg gctcaacaat tgacagtata
     1861 tatgatcttg gtgccggaac ctatactatt acaatacagg aagaggatgg ttgtgcctgg
     1921 gaggaagatg ttgtaattac cgaacctcca ttactcacgt tggataccgt tctaactgta
     1981 gacgtaacct gtttcggtgg atctgatggt gttgtgaacc tcaatcccgg agggggcgta
     2041 ttgccgtatc agtttagttt cgatggtatt aactggcaaa ccgacagcaa ttttatcagc
     2101 ctcgaagcag gcacgtatac gtttacggtg agagacgcct ttggatgcac aacgtcgttt
     2161 accggagttg taatcagtca gccgccacaa ataatggttg atgcaggacc cgaccagagc
     2221 attgcatatg gtcaatcagt ggtattgctt ggaacaaccg gagctcccgt agtaacagcc
     2281 atcagttggt cgccggtaga ttatttgtcg tgcattgatt gccttcagcc tacatgcacg
     2341 ccaactttca attcaaccta tgtgctaacg gtaaccgatg cgaatggttg tcaggctagc
     2401 gattccgtaa atatttgggt ggatatagat tttgtggtgc caaatgcctt ttctccgaat
     2461 gatgacggtt taaacgatgt atttaatata tcaaccgacc tattgatcag ttatgacatt
     2521 cggatatacg accgctgggg ggctgaagtg tttcattcca ccgatatcaa tttcggctgg
     2581 gatggcaagc tgggtgggct tccgcaggaa ataggcacct atgtgtatgt gatcaagtct
     2641 actactaccc taaacacccc gattactaag tccggaacca tcactttgct ccgttaagga
     2701 gttcttgacg ctggtatgcc tgggttggtt cacgaccgga taattttttt tcattttttt
     2761 tttggttttt tggtcgaagt ggctagcttt gttactgagg acttaaaagt gcttatgcca
     2821 taaggctttt agtgtgatct accccctccg cacactttac cggaattccg gtaatttgca
     2881 tgtaaaatgc acggaaaatg gggaaaaatt ccacaaaacc tgcataagta cttgcatatg
     2941 aggtacatgc ctcttagctt tgctctcgat tattatatga cattttttat ttaactgaat
     3001 tagcttttaa ccaccttaat taatacgtca tgattagtag aaaactcacg attgcgatag
     3061 tactgctcta tagcggtctt actttcgcac agacatcttc ctttccccct aaaccaaccg
     3121 accaatggca aataggtctt aacctcggtc tccctatcat tcagggcgat ttgagagctg
     3181 aacctttcgg cggtaaccac gctcctgcat ttggtgttgg tttgaacgtt cgtaaaagcc
     3241 ttggctacat cgtttcattg cgtttgcatg gaatgtatgc acagacctat ggaatggact
     3301 acgttcaggg atcttatgct tacaacaaaa cattgaacgg tgcattttac gatgcgatca
     3361 atgttgacgg ccaaccggca gagaactatt actctacagg tgccaactac accaacaacg
     3421 tttgggtacc taactacaag actcaatttg gtcatggttc attggatttc attgtgaatc
     3481 tgaacaacct gaacttccac cagcacgatc ctaggttcta tgcttatgca tatgccggcg
     3541 ttggtgctat ggcttacaat accaaatatg atgcacttga tgctgatggt aatccttatc
     3601 cattccaaac catcctcgaa ggtttcggat ctcagaacac ctctcagaaa gaacgtctcg
     3661 acgcgttaca tgctgccctt gatggtgagt atgaaacaca agccgaggta gcgaaccctg
     3721 ctgctcgtgg tgctgatgaa gttcgtgcta cttgggtgcg aaacccttca atctccggcg
     3781 gtttcggtgt gtctatgcgc atcggttcta aaatggaatt gggtctcgat caaagagcta
     3841 ctcactactt cgatgacctc gctgacggtc agcgttggca gtatgatggt acatattccc
     3901 cacactacga cctcttaact tatacttctt tgaactttgg ttataacctt ggtaaagctg
     3961 agtctatcga tcctatgtgg atggttaacc cacttgctgt tctgtatgat caaatggctg
     4021 agatcgatcc ggacaacatg ttgaaagata ctgacaacga tggtgttatc gatcgtctcg
     4081 acaaagaacc aaatactcct gaaggaactc ctgttgatac tcacggtgtt agcctcgata
     4141 gcgataaaga cggctgccct gactcagaag accctgaacc attctcttca cctgttcttc
     4201 ctatggaaaa ttgtcagaac gttcacctga ctcaagaatg ggtagaaagc tatgttgatc
     4261 agcgcctttc taaatacact cctggagcaa gcacagactg gatcctgcct tatatcttct
     4321 tcgatcttgg caaatctaac atccgccctg acgctgttcc tgctcttaac caggttgctg
     4381 acctgatgaa acgctacgac aaattgagcg tagaagttgt aggtcacact gatactcgtg
     4441 ccagcgaaga attcaacatg aaattgtctg aagaccgcgc tcgtaacgcc atcaactacc
     4501 tgacaagcaa aggtgttcct gaaagccgcc tcaaaatagt ttacaaaggc gaaactgaaa
     4561 accttatcaa agacgcgaac gaagaagcag aacaccaaat gaaccgtcgc gttgagttcc
     4621 acattattaa aaactgattc atatatataa tcatgacagc caaaggggtg cagcaatgca
     4681 cccctttttt ttatttatat aaactgatat ttctaattaa gcttaaaatg cattaaattg
     4741 catattacaa tttctccatt tttgttgcta cgatgtaaaa tattggcgct tgtttttgtt
     4801 gtgtatctgc taaatccggt acatgctcag aataacgata atgagtcgaa agcacacaag
     4861 catcggcgat tgtttcaccg gcaagttatt tgtgtttatt atcctaatcc gctttcgtat
     4921 ttatacgatt ttccggcata tacttctgaa ttggatacga tggatagcga tatggatggc
     4981 gttgttaatc gctatgataa ggaaccgaat acgccagctt tatctcctgt agacattcac
     5041 ggtgcgagcc tcgatagcga tagagatggt tgtattgatg cggaagaccc cgagccgtat
     5101 tctgatgtat ttgattcaat ggttgattgc cataatgttt ataaggaaat tccgctcaca
     5161 atagaaagtt gtgctcacct gcgactagat aatcaattgt caggtgcaga tgattggata
     5221 cttcccttca tattttttga cgcacataaa gctgatatca gacctgatgc ggaagcggcc
     5281 ttaacaatga ttgctgaaat aatgataagg tatccgatgc tgagtatcag ggcgtatgga
     5341 ttttcagctg gtggcgtcga gaaagattcc tcagcactgc tggttgaaag gcgcataatt
     5401 agttctgtta attatctcat tgaacttggt gttccggaag acaggatttc cgtagtaaaa
     5461 tatagaaagc caaaacagat attttacgac ggtgatattg tagaaggttt tcctcgtata
     5521 gttgagtttt ggattgaaag caactaaaca cctaaggaag tgattctaag atgtatctat
     5581 ataagctaac ctgcaatcta tagcgcatga aactcactct tttatggata ttaatttgcc
     5641 ttacgcatct gtgttatgca aaaaacgcat tatcggattc agtcaaattt aattcctatc
     5701 gggttactgt tgacattaac gaaaatcaag ttattttctc gttttttcca cttaattata
     5761 ttatttgcca ggtggatttt gatccggatt cactcttgtt ggatgatgat aatgatggcg
     5821 tatataatac aatcgacttg gagcccaatt cagatgattg ggccgcagat atacacggag
     5881 taacattaga ctcagaccac gatggttgtc ctgaccactt agatgaagaa ccttattcaa
     5941 gccctgtatt gcctatggag aattgtgtga atgtaagcat aggctgcatt tgggatgttg
     6001 gagtatcagg tagagaatat gtggagcatg catacactac ctggtgttta ccctatattt
     6061 tttttgatcc ggcagaagct gttatccgcc ccgatgctgt tccttctcta aatcaaattg
     6121 ccgacatttt aatacgctac gataaattaa ccttacgtgt tgttggttac agcgattcca
     6181 catccgatga aggagtcaac gtaaaattat ctgcagatcg tgccggcgaa gtcgtcaact
     6241 acctgcattc aaaaggtgtt cctgaaagcc gcctgatagt tgtttataaa ggtgaaactt
     6301 cagaccatat caaaaatgag aaaagtgtgg tagaacacca attgtaccgt cgcgttgaat
     6361 tatattttga aaggtaggcg agaggtaatt attttggatt caatcatggg ggaatgaatt
     6421 aattcataag ccgcaaatcc gatcaccatt acatattcta tccaaacaaa ggtcatgttt
     6481 tcaatgtagg caggagtgat ataggtgtag taggataaat agattagcag agaccataaa
     6541 ataggatacc tcagtccgct taataccgat agcaatatca atggtgccag gtaccatgga
     6601 tgaacggttg ttgcaagaag taaataaata gaaaaggcta tcagcatacc cgcataccaa
     6661 tactgttttt tgtaaaccgc actgaacacc aaaatcaaaa atgctgcaat aaagggtagg
     6721 cttccccgca tattgctgaa agcttcgtaa ttgacatcct tatcgaagaa atatttgccg
     6781 atataaaata tggatgcgtt gaattcgaat tgctgaaaat agagattgaa gctgccggtg
     6841 atttttgaga actctaccaa catgggtaat agaaaaataa ttgccgtaac tcctgcgata
     6901 actgaataac gcatggtttg tttaaaaccg atttgacgaa ataacaacgg catcaacatc
     6961 aaaggccata gctttgttgc aatggcaagt ccaaacgaaa ttgccgatag ccaaagattg
     7021 tttttgttaa aataatatat tgacaataat gagaagaaaa ccattgtact ttcaagatgc
     7081 atatttcctg aaagctcaac aataatcaag gggtttaaga tataccaggc ggcatgtccg
     7141 acattgatat gtaattgttt aagcactttt ggcaatagga atatggttcc gcattcaaaa
     7201 gcaagtagta ttattttgat taccagaatc tgtatatccc aattactgcc accaatgagt
     7261 gcaccaatgg catagattgc ttgcgcaatt ggagggtaaa tactgatata ttgggctgaa
     7321 tttaaactat taaataaatg atcatccaag gtggaaatac caaaatagcc ttcagcacgc
     7381 acttgttcag gaattgatgc ataagggttg aagccatgtg ttacaagttg accatcccaa
     7441 agaaatctaa aatgatcgtc ggtgagattt ggtaatgcaa aggtggcaat caatcgaagc
     7501 aggataccgg cgataattat ttcaccaaga taatttgttc tttcactgct tcgtgcgagc
     7561 aagaaataca gtgcaaacat gccggcgtaa atcgcaatta actgtataaa attttctcgt
     7621 gaggtgaaat acaataatat cgtacacaaa aaaccgtaca acacgtaggc ggctatttgc
     7681 catgtacgag ttgtacggtt ttccattagt tattttttag ttagaccttg cgtgtttgaa
     7741 agagaaatag aagatcaacg caaatcccat gaatgccatg atatggaatg gcaacagtcc
     7801 ataatcctga taatagatac ccatgataat tccagaaagg aaatacaaac tgaatatacc
     7861 ttcaagatag gtaataggac ttacacgcga tgctacatat ttctttttct tccagatatc
     7921 gcctgagttg ttgattgcaa atttaggtgt gcgaacaaat gctgtttgtt gacctaacca
     7981 tccgcgaaaa actgcccatg cattatgcag gctcataccc attgtgatgg caaggaaaac
     8041 agggaagcgt gaaatatata ctttcacggt ttgtcctaca gttttctcgc gttgcagggt
     8101 gctgatataa taagcatatg cgatggcgat agagcccatt aagaaaatag aggcgtattt
     8161 aaaataatta aagtcgatat aatgattttt aataaacagc agtggcacgc tcaatattgc
     8221 tgtaatcaaa ataaaaatgt aattcgttgt attaaataag tgcgcgattg catgcaattt
     8281 aattcgtata ggtaattcgg cacccataac acggcgacca attttgcgcg ctgtttctgc
     8341 accacctttg ttccagcgga attgctgtga tttcagtgca ttcatatcgg taggtaattc
     8401 tgcgggagca actacgtctt ccacatattt gaatttccag ccttttaatt gagcgcggta
     8461 gctaagatcc agatcttcag ttaaggtatc actgttccat ccacctgcat catcgatagc
     8521 agttcgacgc catataccgg cagtaccatt gaaattgatg aaataaccgg cggcattgcg
     8581 accttgttgt tcgatggtga aatgcatatc caatgcaaat gcctgcattt tagtgaggat
     8641 agaataatcc ttgttaatat gttcccaacg cgcttgtact acaccgatct tttcatcggt
     8701 aaggaaatac gggatggttt ttttcaggaa gtcgggatat ggcacaaagt cggcatcaaa
     8761 aatggccacg tattcgcctt ttgcagtttg caaaccgtat tccagggcgc cggctttaaa
     8821 tcctttgcgg attgggcgtt taacctgaac aatgtttatg ccttgttgct tatagaaatc
     8881 taccttttta cgggcgatct caaagctttc gtctgtagag tcgtcgagta gttggatttc
     8941 gagtttatct tttggataat cgaaagcggc aactgcgtcg ataaggcgtt caacaacata
     9001 gagttcgttg taaacaggaa gttgaacagt aaccattggg tattccgatt caggaccttt
     9061 gaaggttgct tcttcggctt ccatacggcg cttatagcgg atgtagtgaa tagccaggct
     9121 caactgcatg atacagtaga agaaaaggag tgtgatggca atggtgtaaa tgacgatgat
     9181 ggtgatcgac atgtagagag gttaggttag taatatttta aaattgttgt aatgatcttg
     9241 tatccggcca tgatggttcc tttcactgta cctgtgacct tcgaaactcc aattcttggg
     9301 cgatagttca ccggcacttc ggtaaagcgc agtttctttt tggctgcctt cacctgcatt
     9361 tctaccgtcc atccataatt cgtgtcgcgc atttcaagcg attcaagtgc tttccaggta
     9421 atagccctga aaggtcccag gtcgctgaat cttgctccaa acaaccagag gatcatgctt
     9481 gtggcaagcc aattgccgaa gatctgctgt ggggccatgc ttcctttaat gcgagtgcct
     9541 ttttccctgc tgccgatcac catttcgaac ccttgttctg taatcggcct gatcaggtca
     9601 ggcatttgct ccggatagtc ggaataatct ccatcaatga acactactat atccggttgt
     9661 tgctctaccg gttttctcct gatatgggct atgcccttca aacaggcaga accatatccc
     9721 ctccgcgctt ctaacagcac tgtggcacct ttgctccggg cgatttctgc ggtattatcg
     9781 gttgaaccat tgttcactac gattacctct tcgaccagtc cggcgggaat atcatttaac
     9841 acgtgaccaa tagacccgct ttcgttaaaa accgggatga ttacattgat ctttgccatg
     9901 gcttttccgt tcccgtgccc ttctctgaaa aagtgcacaa agttaccgct tttaaacagt
     9961 ataaacctaa tatgtcagga gagaattttc aagtcgtcgg atgtgaataa cacctcagtc
    10021 cggcttggat tttcggggcc tgtttccagt tttaggacac ctcttgggca tacggcagca
    10081 caaacgccgc agccgacaca gcttgctctc acaatatttt gaccacgctg ggcgtaagca
    10141 cgaacgtcga tacccatctc gcaataagtg ctgcaatttc cgcaggatat gcactgggcg
    10201 ccgtttgtag tgatgcggaa gcgcgatttg aagcgttgga tgatacccat gtaagctgcc
    10261 atcgggcaac caaaacggca ccaaaccctg ctacccataa gcggatagaa acccacgccg
    10321 atcaccccac tgaataccat gcctatggcg aaactgtatc ccttggctat ccattttgcc
    10381 gaagaaccca gcaattgatt gtgcatagaa ttatctatga gcaaagctgc cgtaatcaaa
    10441 gtgatagctc cgagcacaga gtagatggtg atacgttcaa ttttccaggc tttcaggctc
    10501 ttatctgaaa gatggcggaa ggggtctcct gcagtttctg ccaatccacc gcatccgcat
    10561 acccaactgc aataccatcg cttgccaaaa aagtaagtga gtataggtgt ggcgatgaat
    10621 gccattactg ctccccagaa taaaaagaaa acacccaagt ttccctggtc tttgaaataa
    10681 tttatagaat ttggaaagaa agcatcataa tcaagtggcc agaaataact gtaataaggt
    10741 tcgaaattat agagcgcttt cattacggca ggtataatcc atgcaaatcc aaattgaaaa
    10801 aacattaccg atatggtgcg aattacctga tatctgttgt gtcggtattt cagtatgaat
    10861 ttaatgccca tcacaaaaac agcgagcgta tataaagaac cataaacaaa ccattgatcc
    10921 gcaggtcctt tccgcatcca ttgactcagg ggatcgaaca tgcttaccag accggcaaaa
    10981 acatcggtat gtaaaacaga tttccagtag tcgcgccagt ataatgcaat ataaaatgca
    11041 gtaatcacga ttccggaaat ccatccaagc gttccacgtg atgagattga tgtaaagaat
    11101 gcgcgattat tttttatgcc ctttgggaga tgaccatatg taccaaaact ataggtaatg
    11161 ccaccaataa taaatcctgc caaagtaatt aacaggcaga tcagtggttg ttggtttccg
    11221 acaccgaacc aggctataaa aaagaataag ataccgattg aagtaatggc gattccggtt
    11281 ttttgtaaac cattgaagtt cgcggatgag ctgcctgaca gcgacatgtc tatttgttga
    11341 tcagccatgg tatttatgcg ttgaaaatga gtgatcgaat tgttttttca tcagcctgtg
    11401 taatgcgatg gtcggggaat tgtgcatgcc aggcatcaat aatattttct tcgtagcggg
    11461 aataaaattc cggatcaaat tgtgcagcac gcaaatgcgc catcacatat tctacacttc
    11521 gcgattcttg caaccatgta ttaaaaagtt tatgtcgcat cctgatacca aatgtattaa
    11581 tgccgataaa cttatgatca ctgctattga atacgatatg tacgcatttt tgtcgatcgt
    11641 tatgttccca ataaaattcc gattcaccgt cgcgtaactt actaagtacc gtgccgtatg
    11701 tttgatattc aatgtcaaga aattttgcgc tgttgaacca gtagccggga tcatactcag
    11761 catttattcc caacatattt tttgcggcac attcgccttg aatttttccg gtgtaccata
    11821 cttgctcaac aggcctacga tcagggagag tctccgtaaa ctgtgcgcaa tcgccggctg
    11881 caaaaacacc ttctacggag gtttgcatgc gtttattgat cagtacgcct cgatctgttt
    11941 taatttctgt tccttgtaaa acactgatat ttggtgaaac accggccgtg aggccaacta
    12001 cctgacaagg aatttcttct cccgaatcag taattattcc ggttacgcga ccgtttgcat
    12061 cacctttaat ttcttttaag gtggtattta acctcagatc gaaaccatga tcctgtatat
    12121 ggttggtaat caaagcagat tcttgtgccg gcagaatatt actccaataa ttggactctc
    12181 gaacaagaag ggtgacggga taatgccttg catgtaacat ctctgccatc tcaattccta
    12241 tcaatccacc accaacgatc actgcgcggt taataccatt tcttgtattt tcctctaatt
    12301 ctaacagatc ctgataactg tatagcccct gcaccccttt aaggtcttga cccggccacc
    12361 cgaagcgatt ggatttcgag cctgttgcaa tcaataagtt atcatactgc atatgatcac
    12421 cgctgtcgaa atgaagtgtt ttttttgcac tatctatttt acttacccag gctttcttta
    12481 gggaaatatt atttttctca aaaaagtctt taggatatgg ttgtgtatcc ttgaatcgca
    12541 tatgtcccat gtatatatac atgagtgctg tgcggctgaa aaaaagatct gtttcacctg
    12601 agatgaccgt gatatgatcc ttactcagtt ttcgcagcgt tcttgccgct gtgataccgg
    12661 taataccatt acctattatt acatgatgca tgagtagaga ggttgcctgt taaagacgca
    12721 tggtgaaaat aggaacaaat gcggaatagt tcgcgtatga agtataaaat tttgttaaac
    12781 ctggattgcc agacaggaaa agacattatt ttccgccgtt ttgctcattt aatttccagt
    12841 tgtattccat gaattcaatc ggtgtgtcgg cagcaatttt attgttgctg tattgattga
    12901 tgaaatcctt caatgagcca tttttcgtga aatcgccggt gaaccagttg aaaatactgc
    12961 tcagttgtgc tgatccgttt ttgatgttgt tgcgcgatgg atcgttgatg aaatcgcgtg
    13021 cggccaggtc gagttgcgta tttagatttt cggctgtata agcactattt cggagcttag
    13081 ggcatgaata acttgcacaa ttaattgcaa aatgtatgcg tggttcctcc attttgcgta
    13141 aaatattgtg ttcgatatta ttcaaatcaa gttcctgtcc ttctatgtta atgaattgaa
    13201 catcccatac ggaattgata aatgctactc cgggcttaat gtctttgatg ctttcaattg
    13261 gatagtttcg aatgatcaat tgaactgtat aggcattata tgcatttatc cagtaagcca
    13321 ggatttcctc gcgtgtccag tttttttcgt tgggataacc tttttccaat aattgcagat
    13381 agtcgtttaa tgcagtgctg tcgaggataa agcctttata atcgacaaat ccattatcat
    13441 cgacatgttt gtccaataat gccgaccatt gttcatgtga aaccggtttg ctgttgctgt
    13501 cgataccatc tatcgttctg catgccggta ataatactac agcaagcatc atcccgatga
    13561 gggtgcgata aataaggttg ttgtgcttca taatatcaac atatacgctc agacaagtta
    13621 tatggttcaa aaaaaatccg cctttcatat gttggcaaca tacgaaaggc ggtaaatatt
    13681 gcattgggtt ttactgttta attatttgtt tgctttcggt tgttccgttt gcaagcgtag
    13741 tttttacgat atacataccg ggtgcggcat cggagatatc cagtactatc cagccattat
    13801 cgctttcgat cacttcctga cgaatgcgat tacctgtcat atcaaatact tccatagtga
    13861 tgccaccatt aaatgcattg aggaactcca tttggaatac tccctctgat gggttcgggt
    13921 atatattcag gtaataagga ttttcttcag gagaaggcaa tcccggcata atattcaagg
    13981 tgtaatcttc cacttcgcca tttgcaaatg tttcgcaact cgttggataa ccgccgtatt
    14041 tgcagcttat gcgcatgcgt gtagtacctg ttccagccgt agtaggaatg gtaatcgttt
    14101 tatttaagtt gccggcactg gtagcctttt tttggaatac ttgttcacct gcatctgcga
    14161 aatcgccatc gcgattaaaa tcaatccatc ctctccaata catttgataa atagtaccgc
    14221 tgaagccggc gctgtaagta agtgtatagc ttgatccttt aataacgttt gcactcattg
    14281 cagtgccgtt gtagtatccg ccatcatttc ctgatgcgcg tgttattgtg ccaattttta
    14341 cgtaatcaat atagcgtgat gctgcgcttg tacctgcgct ggtgcaatag gtaactacag
    14401 gaacaaatga tgctgtataa tcttccactt cgccataaga aaatacttca catgggccgg
    14461 caagtgcatt atacttcata ctgatgcgca tgcgtgttgt gcctgtcatt cctgcggcaa
    14521 cactaaagct tcctgaaact gcggaagtaa cagcgcctga attatataca ttctcaccgg
    14581 catctgcaaa atcaaggtct ttattataat ctatccaaat tgaaaaatat tcaggataaa
    14641 ctgtaccggt gtaaccggga gtcagtgtga atgatgcagt acctccgggt gtaaggttaa
    14701 tgtttgttgc agtgaaatcg gcatatccgc tgttgttccc tgagttgtta gttgtacctg
    14761 ccattgaaat tgtttgtatc cattcgtcgg cagttgagct tccaacgctg gtgcaataag
    14821 ttggaacccc accacatgat gtgatacatg ctgcagcagt aattgtattt ctgattgcat
    14881 ttccgggctg agtgccgaag ccattactca aattaatgcc atatcctgta agatggcaat
    14941 aactcattat cgttccacca ctggttggtg ccgggccggg agggcaacca ccttcagttg
    15001 tgtaacaatt atctaaagca ccacctaccc atgcagagca attatgtgta tgataacttc
    15061 cgaataaatg tcccatttca tgtgtcacca ccataactgt ccaactgtaa gtaggaaatg
    15121 cagaataggt tgcatcaata tcgctgtagc aatatttatt tgaattgcat aatccgttga
    15181 tcgttgcagc aatgcctcca agtccgccgg gatcaagcgt tacaaggtga gcaatatcgc
    15241 cggtaaaagt tgttcgaaag gctttaaatg aattcaacgc atcatatgat gaagctgtag
    15301 cataaccatc agccgatgtc cacacaaaaa tttcagatat ctgtgtcgga acagtttcgt
    15361 tggtataaat gatagctgca ttattgtaaa caccggtaat ataattagtg gtattggtaa
    15421 cactcccttt attttgatac aactgataat cgcattcaaa ataaattttc gggcatttgg
    15481 taatacgttc gccaccgcca ttttccataa tctgatgaaa ggtgtcgaga tacgattcat
    15541 catccacagt tgcacatcct gcattttcag gttgaacaat aatatctctg tcgctgaaca
    15601 ccaaataatc ttgccagtcg ccatcgccat attttccgat attgatattt ccttcggagg
    15661 aagtggatac catgccgatc atctcatttt caaaaatgct gatagcggca atagaattat
    15721 catcgccctg cacaatcccc tgataatata caccgggcgt atattctacc gcagcaccat
    15781 tgctttcgct ggttgttact ttaaatccgt cggcaaataa atttacttgt acaagttcta
    15841 aggtcaggtt ttgaccttct ttataaggaa ctgtcaatga aatcgccgct tgtttttctt
    15901 caaacaactg atgtattgca tcaccatcaa gtcgcgcgta agtaatgttg ttggcgaaag
    15961 cctcagcttt gctgcgatat gccggagcag atacgcgatc cagggtttga gcaggggtaa
    16021 aattgatctt tgcagtatgg gcatcgcgta ccatttgggt tacgggacga actgcagttt
    16081 gggcaataat tgctgatgtg ttaatgagcc agccgcacaa ggctatagct gtggcataca
    16141 gaaatgtctt ttgcataaaa tgagttttaa aagtggggtg tgcgcttgtc gctcggtagg
    16201 gtgaaataaa gacaactcct tttctttgat ttcaaaatgg ctgtttatac acggatgtca
    16261 caaaatgtgc acttttctgt gtacgactga ttaattatca ataagttata aaagatctcg
    16321 aggttattac cttgtcacat ctcgaaataa aatctgcaac atttatatgt cttgcgtcat
    16381 tttgcgcact accttccatt cgctgaaaaa ctctttggca ataatgcgga aaaaggcgta
    16441 taccggcatg gctgcaatca ttccgggaac accggccagg gagccaaaca ccagtacaac
    16501 gaagaacact tcgagcggat gtgctttaat actgttggaa tagatgatag gttgcgatac
    16561 gaaattatca atgagctgaa caccctgaaa taaaatcagt agttgtatca gcatcggaat
    16621 gatatcgaaa ttcggatcaa cactcagatt agtggttata ccaatcagca taccaaacaa
    16681 cattccgatg atgggaccga tgtatggaat gatattaata atacccgcaa agagtgcaat
    16741 caaccagcta ttggcgccaa atcccaatag caacaatcca actgcgataa ggatgaaaac
    16801 gactgtcgat tgaattatta aaccgacaaa atagcgcgta agatattttt tgctggaatg
    16861 cagaatgcgc cgcccgtttc cttcaagctt ggttggtgtg agtaccatga tgatattata
    16921 aaataatcgt tcaccacgca gaaaaaagaa ggtaataaaa gtgattgaga acagcgccac
    16981 catcacgttt ccgagtccgc ctaaaatatt attgaaaata tgtgtgagcg atgttgcatt
    17041 aattgcttca cgcaatgatt cgtttaatat tagttcaacg gatttagcag gttgagtggg
    17101 gtcctgtaca tgcacgagca tagagtcgag cacttgtact tgctgttgaa gtccgtctgc
    17161 cactttctgg gtatcgatat ggctgatggt atttgtttgt tgaataacca gtggtgtaaa
    17221 tatgctgatg atgccaaaga ggataccata taaaacaccg atagttaaca aggccgacaa
    17281 ggaagatggc attttaaatt ttccgatatg caacttgtcg agcaagtcgg caatcggttt
    17341 tccgataata gaaattattg cggcaattat aaaataaaac agaattgaag tgatctgcat
    17401 caacaaccaa acaacgccaa acaatcctgc caatgtaaga atgacgatga gggttgtttt
    17461 gaaaacggaa ttattgctcc agttgttgtt ttccatgcct ttagattgag gtaggcataa
    17521 agatacgagg cttggaccac aaaaaagccc caactgtcgg gttggggctg ataaaaagcg
    17581 aatatcgatc agcagcatcc gccgccatcg taaaaccatg tagatcctga gataaaaata
    17641 tcatcgcgat tcagtgcagc caggttacct gctgttttat cgcaaacggc aagtggttgg
    17701 tttggtgtca tcacatgtcc tttgtgatcg tcgaaatatt cttcatcgcc gaaatagatt
    17761 gctgttttgc cggtgaatac acaaggtccg tcggcaggca taggatcttt aattgcgcac
    17821 acttcggcac tttctatata aagtaactcg ttggtagcat aatgttttgg atccaaaatg
    17881 cgatatgggc gacgggcgcg cacttcaatg gttccgaaac cgatatttgt gatgcgatta
    17941 atatattctt gcaaaggcaa tgaaccgctc aggcagagtg cccgcaattc ttcatcgttg
    18001 cgcaagtttt cagggatttc ctgttcgcat gtaggatcac tcatcaccaa gcgcgcatgg
    18061 cgtttcatgg tgcgatacat ttccttcagc gctttttcaa ggtcttcatc gcgaaatatg
    18121 ttgaacaagc aattttgtgc agcgatgtca atagaattat cttctacagg taaattaaat
    18181 gcatcgcctt tgcgcaagtc gacaaactcg cgtttaaacc atggatttaa tttttctgct
    18241 tcaagcaaat tttcgtggca agcttgcaac atttcctcta caacatctac acctataacg
    18301 ccacctttgc gacgagagaa ataagcgaat tgtaataatt ccatgccacc accaacgccg
    18361 acatataaaa tggtagggtc atttaccaaa tcgcgagggt taaccgtgct tccgcaacca
    18421 taattcatct ccagcatttt ttgtggaatc accaatccgg gcaattgcca taccggtgtt
    18481 gtggtgcaac ataatccttt ctgcggattt tcagctgctt ctttgtacac attgcgtgtt
    18541 gtttctaaat acgttgtcga catgcttgtt gttttattta atttttcaat tgaattgttg
    18601 taaaatataa aatgttgtag ctgctaaaac tgctgcaatt ggaagtgtaa gtacccacga
    18661 cccaacaatt tttcctatca ttttgtaatc agcttttcta tttactactc cgataccgaa
    18721 aataccacct acggaaacgt gagttgttga aaccggtaat ccgctcatgc ttgctgtcca
    18781 cactaatacg gctgtaacca gatttgaggt aaatccttga ccgggattca tgtatgtaat
    18841 tttgcgactc attgtttcag caactttttt cgcatttaat aatccaccgg ctgccatcac
    18901 tgcggcaaca atgatgatca tggaaggcac gtttagaaaa tgcacgagca ataatagccc
    18961 ggcaattttc ggtgcatcat tcatgcctct ggcgaagcct actgcaccgg cgctgataaa
    19021 atgtaggcta tccaatattt tttgtgcact gataccaacg atatgaccgg tataagattc
    19081 tttaggttgc tgagtgaggg aaattgttga tgcgcttatg ctattttgtg caaagtgcgg
    19141 ttcctgtatt tcaagcgtgg tatctttttc tatgcgcaac caggttctga tttgacggaa
    19201 tagcagatat aatataatgc tggcaccaca tgctataaat gggctgagga tgagtggttt
    19261 taaaaatgtt tcaccaagtt gattaacatt caattcgctt cctaccgcaa taaatcctgc
    19321 accggtaagt gcaccaacaa tggcatgtgt cgtactaaca gggatgccta tcctggttgc
    19381 tagaaatacg gtggctgctg cgccaatggc cacggctatt gcgaggttag ggtcttgtaa
    19441 ggtgtcatca ggaatcaatc cctttccact gaaatttttc attaaccctt gagcgatata
    19501 catcgcaaat aaacaacctg ctagtgttgt gatggtgccc catcgaagag cggtcttaaa
    19561 atttgtggtg ccgctgccaa agagtgtaga tacacctttg aagttgtcgt tcgcgccatt
    19621 tgaataggcg agaaatagtg ctgccagtaa aataataatt gcgatcatgc ctgtatcagt
    19681 gatttaaata aacttatagc ttgtatgtcc tttaattcat cgatatcgct tagtggttct
    19741 aatagatgaa gtgtgccatt tctgctgagg atgtcagctt gcgtttcctg aaataattta
    19801 tgctcactcc agcttttatt ttccaaaacc caacggttcc attttttcat acctatgaga
    19861 taatatccgc cgtcatgtgc cggaccaatc acaaaatcgt gatgatcgag tgcttcaaat
    19921 gcctttgcaa gatgttctgc tgtaagtgca tagcagtcgg agccaatcat tactactttt
    19981 ttatattcat ttttgaatgg aatgctgaaa gcgtaatcca tacgcacgcc caggtcgcca
    20041 ttgcattggg tatatttttt gtatgtgctg ttaggaaaaa tgtcgttgcg ttccactaca
    20101 tgatcataaa aaagaaattt atcctgcgga atcatttctg ttacctgttt ggtgtgttcc
    20161 agcaattgca tataaatagc taaagcggca tcatcaccga tgtcggcagc caggcgggtt
    20221 tttaccttcc ctttgaccgg atttttaatc atgattatga gcgcgtcttg agtcattgct
    20281 attgtttgag tttgttgatt acgctacagc cccaccgcat gaacttccac tgcctgcagt
    20341 acagccgtaa caatgttggt tcaatacaat gttgcgcgat tgtaatgcgt aagtatccca
    20401 tttcgaaata tgatttggtg cacctgaatt cacatgtaat ccgagcatct gattgaaatc
    20461 acaatcataa ataaagccat cccaacctat cgaaaccgta ttgcgacaca ttacgccggc
    20521 tgcggctacg gggttgaagg catcgataag tttttgcata tagctttcat agtttccgga
    20581 ttcgatcaga taatcgagga aacgagagat aggcatattt gttatacaaa acaaattgtt
    20641 gaattctata tcgaaattgc gtttcaattc gcgtttgaac tgttgctcta aagagaattg
    20701 agagccgggt agaaaggcgc ctcccggatt ataaaccagg tttaaaatca acccggtgcc
    20761 ttctttaccg taaccgactt cgttgagcat ctgaagtgct tttatgctgt cttcgaaaac
    20821 gccatcacca cgttgtgcat cggtgcgcga tttattgtaa aacggcaatg acgaaaccac
    20881 ttcaacttta tgttcggcaa aaaattgtgg aagatcatgg tattttttat ttgccatgat
    20941 aatggtaaga ttgcaacgca ccattgtttt tcttccgagc ttagtgatct cttcgacaaa
    21001 ccatctgaaa tcaggattca tttccggagc tccgccggtt aaatcgacag tagaaatatc
    21061 cgtttttgca ataatatcca ggcattgctg cattgtttca cgcgtcatga tttcttttcg
    21121 atccgggcct gcatccacgt gacaatgcgc acaagtttga ttacacatat agccaacgtt
    21181 cacttggaat atttcaagct ttgtaggctt tagcggcagc aaaccaatag cctttaactt
    21241 atcgtcgaac cgttcaaagt cggcacccgc aacctcatgc gtttcaagca cttttagttg
    21301 gtaattggtg tcggcaagtt tatgatgctg cgcgtgtaat gattttgagg tcttcatact
    21361 ataacgtcgc ccctctggag cttttttggt ggtttgtgag ttttagtgcc atgctaggta
    21421 ttggtatggt gtggtgtgat gtgatgtgat gtaatgtaat gtggtgtgtt gcggtgtgtt
    21481 gtgtttaact gacccacccg cctaccggcg aggtaaactc acccactgac tacatggata
    21541 ggtctttgaa cttattcatc atctgtgtgc catgtaccaa tgatgcgcca ccgcggatag
    21601 ctgcagcaac atgtatggct tccatcattt gttcttcgtc acaacctttt gccattgtat
    21661 cttccgtata agcatcgata cagtatgggc attgaatggc atgcgcaaca gcaagtgcga
    21721 ttaaagattt ttcgcgagct gttaatgcac cttcttcaaa tacttttccg taataagaaa
    21781 agaatgcgcg gcctaattcg ccatcccaat ctgatatttt tccaaaattt ttaaggtctt
    21841 tcgggtcgta gtaggtactt ctttccataa tgatattatt ggtcttataa attgaataaa
    21901 ataaaaaatg aattacgaca tacgattaat gtatgtgcgc cgataaattg taaagaaaca
    21961 tttcaaaata ccggaggcgc ccttaaacct acatacatac gaggttgaga cagtgtagga
    22021 tttgtttgat ctttattgat cacggcatat aaacctgcga gcagggaaat taatatgaaa
    22081 cgcagtgatg tggaaagctg cgttcctttg agcacttgtt gcagtgcagt aggataatcg
    22141 atatcagata atgctctgct ggtggcaaat gatatgccgt ttactgaaaa atattccgtg
    22201 agcgatacaa acaaattgtc tgatgaccat ggtaattttt caaaatacag cctatcgaaa
    22261 tgtttgcgat gcaatccgat aaggtataca ccgccatcat ttgccggtcc cataactgcg
    22321 ccgtgctcat cgagtgctgt tgcggctttc aggaggtcgt tggtggtgag cgtgggacag
    22381 tcgttaccta tggcaattac ctgagtataa cccaggctgt aaatgtcctc aaaagcatga
    22441 gccagttttt caccgaagct gttaccatgt tgttgggcgc tgctgataac aaagcaggga
    22501 agaccagtat ttctggcagt tcgttctgcg tgaccgatca ggtgaccggc gatcaggcta
    22561 tttgtgcgaa ggctgtgtgt ggcagaaaaa tcttttgcac gtgcttcctc ctcgcgggtg
    22621 cgcgtaaaca acacaaaagc catatgtccg ggatgcctga aatccatgga accaacttaa
    22681 caacccctaa gggtcaatag ttcggctaaa gtagagaaat taggatacct gtcaagcgac
    22741 ctttccctaa ataattgtta atacaaccat aatattgtca aattgtgtcg agaagaaaat
    22801 aatcgtaata ttgcgataat tattttacct atgggtatta gtaaacttga ggatttcact
    22861 gtgcgagata acaaggtctc tgcttatgcc aaggctttgt cgcatcccgc cagagtagct
    22921 attcttcggc tattggccag caggagttcc tgcatgtgtg gcgatatcgt agatgaactt
    22981 ccgctatcgc aaagcaccgt ttcccagcat cttaaggagt taaaagaatc cggattgatc
    23041 aagggatcca tcttgggtgc caaagtatgc tactgcatag atccggtagc ctgggaggca
    23101 gctaaaagct atataaatgg actgttcgac gattatagcc cctgcataga tctattatgt
    23161 taaatgaata aaaatatcat cgttatataa cgatatgatt ccccggccaa accatgaact
    23221 caattttatt tgattaaccg gtaaaaagac gctaaccatt tcacaacatg aaaattgctt
    23281 tgttctcaga cattcacgcc aatcttcctg ctatggaggc ttgctttgct gatattgata
    23341 ctcgcaggcc ggatgcagtt tattgtttag gtgatctcgt tggatataat atctggccca
    23401 atgaggtaat tcgcttaatt cgagatcgcg gtattccggt catctttgga aattacgacc
    23461 aggggatagg attacacagc gatgaatgtg gatgtgccta taaaacggat attgaaaaag
    23521 aaatgggaaa gatttccatt tcatttacca atagtattgt tggagatgat gagcggaatt
    23581 atctgcgttc gcttcctgcg catatcagag tagatcattt aatacaggaa gagacaatga
    23641 gtttgttatt ggtacatggc agtccgcgtc gtgtgaatga atatttattt gaagaccgtg
    23701 aagagaaaag catgttacgc atcatgcaac aggcgaatgc cgatatcatg tgttttggac
    23761 atacacataa accatatcat cgcattttac aggatggaga ccgttatcgt cacgccatta
    23821 atatcggatc tgttggaaag ccaaaagatg gggatccgcg tggctgctat gtaatgctta
    23881 ccatcgacga gcatacttca gttcatgctg aagatgctgt tcaggttgaa tttgtgcggt
    23941 ttcaatacga tgtagaaaaa gcagcgcatg cggtggaaga aagtccgctg ccaaatgcct
    24001 atgcagatat gttgagaaaa ggatactgaa tatgagaaat tatgtagctg aagcacttgg
    24061 cacatttgcc attttatttt ttggaaccgg agcggctata gtagatagca taaccggagg
    24121 caccgtttcg catgttggta tttctgtcac ctttggattt acggtgatgt cgatgattta
    24181 tgtgtttgga aatacatccg gcgcacattt taatcctgcc gtgaccattg cattttattt
    24241 ttcaggcaga tttcccggga aagaaatatt gccttatgtg attgcacaat tcctcggtgc
    24301 gattgcgggt agcggattgc tattattcct ttttccagat gccgctacca gaggtgaaac
    24361 tgttccttct ggagatgttt tgcaagcatt tgttttggaa tgtgtcctca cattttttct
    24421 catgctcacc attattcatg ttgcacatgg ttctaaggaa cagggcatgt tcgccggatt
    24481 ggcaattggc tctgtagtat taatggcagc attatttgca ggtcccattt gcggtgcatc
    24541 catgaatccc gcacgttcac tggctcctgc actcatgtca ggaaatttgc aaagcatctg
    24601 gatatacctt actgcccccg tactcggagc cgttggcgca gtgttggggt ggaagtggtt
    24661 gaggcggtga gtggtgagtg gtgagcttgc ctcgccgata ggcgggtggt gagtggtgag
    24721 tgcaaattcg ctcgctttgc gagcgaataa ttacattagt ctcactactc aatacccact
    24781 actcactaca gcatcgtgaa tcgtgattcg tgactcgtga atttttacct gatannnnnn
    24841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngatcac gcatgatccg aaagcgtttt
    24961 atccagccac acgacacctt ttttaatata cttctcttta tcgccgcgat gcatatcaat
    25021 gatctttttt atgcgcggat gccatttttt gtctttacag agatcccaaa gtatgaccat
    25081 cgataatctg cgttcccaca tatttgaaga aaccgcaaat tgttctgaaa ggctcatgat
    25141 gcgttcggga tattttttgt tgagtttttt taaactcttc ataccaatgg tatcacaaat
    25201 cgcccaatta tctatttccg gactgaagcg tattacagtt gcgatgccgc gttcggggtc
    25261 tgttttcgat tgccattcaa gcaatttaac cgccagcatg cgcgcttcat aaatgcctgt
    25321 ttcccataat gcatcgacca attcaaaact gccgggtttt attgctttga gcagttcgtt
    25381 catttcagga ctgcgcacgc catagatctt ttttgctccg ggcgccgtta ctttcatgcc
    25441 ttctgcatgt gatggatcag catgttcctt taaataggca attatttctt ttatagtggc
    25501 tgcggtagtg ctcatgctga ataattctcc agataactat taaatgattg cattaattcc
    25561 tgtgttacat agccgggttt accgttgccg attatacgat cttcgatgcg agttacaggc
    25621 atgatatttc ttgttgttga agaaataaat acttcatctg cttgaaataa tcgctccatg
    25681 gtaacaattg cttcgattac cataaagttt tgaagtgcac aattaatcac atgctttctg
    25741 gttacaccta gaagaatatg ttcgagtggt gtgtaaatca atcctccttt tacataaaaa
    25801 atattgcatc gagaacattc gcgcactaac tcgccgacat aaaataaaac atcatcggcg
    25861 ttctccgcct gcatgcgcgc aagctctctg aatgaaccaa gataaaaggt ggttttatat
    25921 tcgggatatg gtttatcgta ttcggaaata atcagtttta tgccatcgga ataggaagat
    25981 gcaggatagg ctttcatgac atcctgatac acagtcagca caggcacggg aataccattt
    26041 tgcatgcgac ctgttatcat cactttaaca tacccgtcat ctaatttatt tgcttctaat
    26101 aagcgagtga ttacttcggc caccaacgac atcggcaaag gctctgcaaa ttttaatacg
    26161 gattgtgaat ggtcgagtcg ctctaaatga tctttaagaa acgggataat gcctttgtgt
    26221 tgccgcatgt agtcgaatac accaaatccc cgttcaaagc ccagatcatc gagccctata
    26281 cgaatatccg atttattcaa atattgtccg tctctgtatg caagtaattc actcatatgc
    26341 caaagatact attccgtgtg atatcaaatt atataggcat tcataatcgt attattcata
    26401 aatatcgcca tcgcaccact attttatacc tttgcaacaa atggcaagag atatttccat
    26461 atcgtcatac acatacgaat tgcctgatga ccgtattgcg cagtttccac tcggcgatcg
    26521 ggcgagcagt aaattgttat ggtataagga tggaaaaatt catgattata tattctctca
    26581 gttgcctgac ttattacctg aaaatgtgac attgattttt aatactacta aagtagtatg
    26641 cgcgagaata ttgttttcag ttgaaggagc gcaaaagccg atagaaatat tcgtcctcga
    26701 accgcagtcg aatatgacaa tggagcaggc tatgttgcaa aagggcaggg ctgtctggaa
    26761 atgtcttgtt ggcaacaata aatcatttcg cgccgatgct ataaaaaagg aaattcaatg
    26821 gcatggcgag caacatgttt tgcaggtgaa taagccgcaa ttatcgggag atgttttttt
    26881 agttgaattt atatggtcag aaagtttgac attctcggaa atgcttgacg ctgccgggca
    26941 gattccattg cctccttata tgaagcgcgg accggaacag gaagatagtt cgagatatca
    27001 aactgtttat gcaaatgaag aggggtctgt agcagcacct actgcgggat tgcattttac
    27061 ggatgctgtt atcaatgagt tgcgaagaaa aaatattcaa attgagcatg ttacattaca
    27121 tgtaggtgcc ggcactttcc gaccagtaaa agctgcaacc atggcaacgc acgacatgca
    27181 tgcagaggaa atttccatca gcaaagaaac attgaaacat cttgtaaata atccagattc
    27241 actcattgca gttggcacca cctctttgcg tacattggaa tcgctttttt ggattggttg
    27301 gaaatggaaa catgctgaac aagaaaatgt tcccatggtt aatcagtggg atgcttatga
    27361 tgctgatgtg cctgttgagt ttacctttgt tgatgctttg catgccatta tttcgcgcat
    27421 ggaaatattt ggtattacaa ctatcacaac aaagactcaa ctattaattg ctcctggata
    27481 tacaataaga accgcaaaag cgcttatcac gaattttcat cagccaaata gtactttatt
    27541 gttgttagta gcaacgtttg taggcgctga ttggaaatta atatataatc atgcacttga
    27601 aaatgaatat cgttttttaa gttatggcga tagttcttta ttgtggcgta aataaccttt
    27661 tcttcataat cttatttcaa acgccatgga ggatcaatgc gattgacgcc tgcatgatat
    27721 atgataatgt gattcccatc cgggtcatgc agttcgcttt cacgccatag ccatggtttg
    27781 tcttccggca tatgatcgat ggagatacct tttttgatga attcattaat ctttgtatct
    27841 acatcatcca cttcgaaata aatacttgtg cctgttggtg ttagcggcga atccggtaca
    27901 tgcaaggaaa aagtgctatc acctgaagga caaagaaaac gtgcgtattt atcgtgcgta
    27961 tgcacaatga gtcgtaatcc caatagctga taaaaatgta tcgacctatg gatatcggaa
    28021 acggtaacag tgacttgatt taaattcacg aggttagtat gattattgtt cccacacggt
    28081 gcgaatgaat tgtgcgcttg taaaggattc accattgatg atgtcatttc ccgaaaagcc
    28141 accaagatat cctaacacat tacaattcgc atctaccact ttggtgtcca tatcatttcc
    28201 gcaagtgccg ggatcgtagc aatagacata tccgccttga aagagatatt cttcaatttt
    28261 gatatcctcg caccatgcat ctttactgaa gtctttaatt gaatcggcaa cacattcagg
    28321 agtattggct gcaggattat atttttcgca accaaataag atcatcatcg cgaggagtgt
    28381 caatgatgtt gatttcatat cagatgtttt tctaaagata catcatcctg tcgattttgt
    28441 gattcaacgg tcgttctcaa taaaattagg ttaattttgc agcattcatt atgtctatat
    28501 ccattaccct ccagacacgt tgcgccggtg tttgtgaatt gtgtggcgcc gaacctgcag
    28561 ttattgaaat aacagtaagt cccaaagatg aaagtaccga aaatcaggta gcgatttgta
    28621 cagtatgtga aacagcgctt gccaatgcgg atgaaaaaaa tcactggcaa tgtttgtcgg
    28681 gaagtatatg gagcacagaa cctgccgtgc aggcattgag ttatcgtata ctttatggaa
    28741 ttcgcgatga aagttgggct gcagaaattc ttgattcagg agcagcagat gaatcagtaa
    28801 ttaactgggc aatgaaagcc tacgaagttg ctgatgtaca ccaggatgcg tatggaaatg
    28861 tattggcgaa tggagacaat gtaattctca cgcaggcatt gaaaataaaa ggcaccagtt
    28921 tttccgctcc caaaggaact caggtgagaa gtattcactt ggtgcatgat aatacagaac
    28981 aaattgaagg caagatcaat ggaactatga ttgtgatctt gaccaaattt gtgaaaaaag
    29041 gataggttga tcaatgacgt gtttcgcgca aaggcgcaaa ggttattctt agcgcctttg
    29101 cgtgaaaaaa ctacgataaa ttatattatg ggaatatgtc ccaaatcaat agacatttta
    29161 ttctgaccca tccgcttttg cagcatcaac tgcaagattt ccaatggtag tgccggctgt
    29221 gagcccggct tcgcagtcga aacggaagtg aatgcgagaa taaatgcgcg actctgatgc
    29281 ttcgtacata tattgattat acagcacctc attttccgga aacatataac ttaatactgt
    29341 tgccgcagct gccgaaaatg tagagtgacc tgatgtatag ctcggaaaat tcggaagtcc
    29401 gaataatgtt tttatctcag gatcagcctg tgggggtcgc ggatacatat aataatactt
    29461 cgtatcccag caactgattc cggcatccat cattgcagta tttaaataag cgaacacccg
    29521 cgccgtacgt agtggattat aggcatgatc gataataggt tctttagcaa ttgtattcca
    29581 gtgtcccgca ggtgtgtaag tgccaaatcc atctccccag aaataggcaa tgcgttcttg
    29641 ctcgtcggtg agatctttac tgaagtcgag caattcctgt cttgctgttt catatgcttc
    29701 agagccaata gcaggaggcg gacccggacg tacagttgta atgtcatcaa cccaccatgg
    29761 caccacatgt ccgaatttcg gagtaatacc gattggtcgt tgcggcactt ctaaattctc
    29821 ccagttcggc caagaccata aagcgttagc tgctgcttcc atctcatcat attcttcctg
    29881 tagtacctgt gcaaatttca tactgtcggt tttcgcgcga ccgataaaaa tatctgcgag
    29941 agcgtgacca agagaatcac ctgcatcaat atcgctttga acattcatag atgcccattt
    30001 tctgctattg cggcaatcat aagccatgtt ggagatatag gcagtttcaa gcggaaataa
    30061 aaattcaagc acgtcttctg caacagatgc tatcacggca tcttcagcgg gataggaagg
    30121 aatgcttgtt ttaggatatg ccggtgaaat tgtttcatca accatataag gtgcacgtcg
    30181 atcatacgta tacttatatt cccatgtggc gatcaaagca tcatagatcg cagcgcttaa
    30241 ataggcataa gcgcgactgg catatggcgg atgagcgaat ggaaattggg ggtaggtagc
    30301 aggaaacacc tgatccggaa caccgtattc gccattttca tcaggcgatg gtgctaaatt
    30361 atattttgcg atcaactcct cggtgatttc tatccatctg atcgttgtat tatttcccca
    30421 atattcaata gcattcgatt gttctgaagt gaggttatta gcaattgatt ttaattcctc
    30481 tatttccgtt aaataggcat cagatgtaac tgattcaggt gtaggaatac ttaccagatc
    30541 agcaccatgc acatacaccg gttcccattc gccacccgtt gaatcaacag aactataggt
    30601 gtaaggtgta atatccagat atcccggcac atcaagtgcg caggctgaaa ttgtgaacgc
    30661 gatcacagtg gctgcgatcc attgaattga gcgaaacata taacaggcag gtatagttta
    30721 acattattgc acaacaatag tagccattgc tactggcatc tcttctgaga tcaacagttg
    30781 ataaattcct gcaggttgat caatatctat gacatgcgac gaacccaatt gcaaatttcc
    30841 gttgccaatc agcatgcctg cagcattata taaggtatat gttgctgaag aagcggtgtt
    30901 gttatattga atagtaacag gatttccggc catgctagga ttcggcgatg taaggaaaga
    30961 agataatcca tcgacattat tgatcgctgt ttcagcaaca gatgctgcat tgagcgtaaa
    31021 tgaataagta ttatcaggtt gataatgatt agtacagttg atctccacat atccgctgga
    31081 tggtatcagg tctgtaaaaa aatcgacacg cacgatctca gtttctccgg gagccaggaa
    31141 aactgtagtt gaatcgatat cgggcgaata gcataaatcg gtgcacattg cagtgaacca
    31201 tccgtccgga atgctttcat cggcgcgaat aaaatcgatc aatacatcat catcactggt
    31261 gttggtcaat gtgatatgtg cggcgatggt attatctact atgccataat acacttcctc
    31321 actttcatct tcggcagtaa atgtaccttc aggggtaaat acacctgtgc caaccaaatt
    31381 gagcatggag tcgatggatg catacatatt atccggctct ttattgaacc atttctgtgc
    31441 gtcgaaaaca gttccattcg gatcgattag ccaggcagga tttggattgg tgccataagc
    31501 agcccaaaat tcattgcatg gtccgtcgat caataccgga acatcgatat ccatggcatc
    31561 aagcatatcg ctaacgatat ctttgcgttc gccataggtt gttggctgtc gatataaaat
    31621 accttcggca taattgtcgg aaccaacatt ttcataaccg aaatacggac tgaaatcaat
    31681 aacgggatgt gcctcaacgg tgtaaaccaa aaagatattg atttgatcgc catagatagc
    31741 ctgcatatcg tttacaacat ccattttgcc gcgaaacaca taacaggtgt aattgcatcc
    31801 taccaacagt actggcttcc catcgttcag tacacctgaa agctgcactg aatcgccttc
    31861 aagagtatat aatttaaaat cgtgtacaag ttcacctcca agtggtcctt cgaggtttgt
    31921 cgatacttcc ggataaatag ggatcgtgca tatcgggtca ctatctgcag gaagcgattc
    31981 ctgtccgata aaagctgcga gaggcacttg tgagtgtccg atcatgatgc agctatagca
    32041 ggcgactgcc agtgtgaggg ctttgagttt cattcctgat agaattgagg ctcaaagaaa
    32101 tggctttcaa tgcttagtag cagtagggtt taaagtactt ttaaggagtt tacaatactt
    32161 ttagttggaa attaacgaaa ggattggaat tgaggctttg tgttggggaa tgaacataaa
    32221 ttgattagcc gattagccaa tgtggagtgc ttttaacgct ggcgcgttaa tagggaatgc
    32281 aattatattg tttaataaaa tttaattccg aagtgaaatt agctccctct ctatcgacgg
    32341 taggagatgg agaggggtga ggcgaggtta gcaagttagc aggtgcggga atacgtgcat
    32401 gtttatctct gcgactctgc ggctctgcgt gaaaaaggcg a
//
LOCUS       DAOQOU010000017        31889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_623_length_31889_cov_5.811962, whole genome shotgun sequence.
ACCESSION   DAOQOU010000017 DAOQOU010000000
VERSION     DAOQOU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 31889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31889
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_31889_cov_5.811962"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLM90_02760"
     CDS             <1..1215
                     /locus_tag="PLM90_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMZ88318.1"
                     /translation="YSVAFRIVFFLGLFLSAATFFIVPYFTDNLFDTSSPNYDQYTFF
                     MQIFAVTILFYVLGGLSEECIKGLKKTRQYSWISQTINQVAAIAFLLLFGLFSKDELW
                     VNLSYCLGVFVAFAIAQYYWIYYLKHPEVGGSEFGRKKDKKEKKPAKDNAAAAVLAVA
                     QQEQPINTGEMLKLSAPMMMAKYLTMIYTWLDILILAWYAPEAMVSIYRVATRLTSLS
                     TIPLVSINSIAGPKFAEAFGKNDQRSLRNNVKQATRMIFWTCIPILAIFLIFPKFSMW
                     TFGKDFTGSEAVTVFVIVSLGQAANILTGPVTVLLNMTGRQNVTMYYALATVVIDVSL
                     NLILIPIYGIIGAAIATAVARTVLNVGCALQIYFTMGISTIYNPFADLWSVFSKKKMQ
                     PVINESKNEEEI"
     gene            1212..2117
                     /locus_tag="PLM90_02765"
     CDS             1212..2117
                     /locus_tag="PLM90_02765"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMZ88319.1"
                     /translation="MTQRWPNFLIVGTARAGTTSLHEFLGNHPDIFMPLQKEPCFFTY
                     VDEQPEFKEARHRYLTNPAEYLAMFEHHNEAVMGESSTPYLYFHQKSIANIKRLVANA
                     DEMKILIVLRDPSERAYSQYMHNRRDLREPLTFEEAIDAEKQRMADNWHFDFFYVDKG
                     YYYQQVKDYIDNFKHVKIVFYDTLEKQPEKLLSDIYQFLGVTQLQEAKMVKRNQSGEM
                     KVKWLKQMMTTRKNPILNFFRKLMSRDTKKKLRNWAKDKLLRYNLKKTEMNPETRRKL
                     IALYSEDIKQLQTLVQKDLSDWLRV"
     gene            2119..2997
                     /locus_tag="PLM90_02770"
     CDS             2119..2997
                     /locus_tag="PLM90_02770"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMZ88320.1"
                     /translation="MSGIKQNFICIGAQKAGTTTLSDILSAHPDICIPPIKETKFFLF
                     DQDYQKGKSFYDQYFSNYRTQSAVGEFDPDYLLFPYTAERIAETLGTDIKILVVLRNP
                     ADRAYSHYLMTKRKGLEPHDFEAALQAEASRKEDLKTRKIFAYIERGMYGTQIAQFLK
                     VFPAENFLFLVFEEDFIKNRQGTVEKVQAFLGVPFKALDVDLHSNEAGEPINDSLNEL
                     VRKPNFAKRILKTILPFKAIRKWMRKAFISQNMQKVSSPKLSEEQRRQLIADYFIQDI
                     HLTEKLTGRDLSLWYN"
     gene            2999..4333
                     /locus_tag="PLM90_02775"
     CDS             2999..4333
                     /locus_tag="PLM90_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88321.1"
                     /translation="MGILTYEKGIYKEPMSVFGKTMILVVFILFTTRILTRTPLVDYY
                     MPFEILWYTFIITFTFIYAMYIFLVRKTVDEIHYYYILIIIMPLWAGFMANLSEGQNV
                     MGGISSQRHWYGISGIFVLIYLLQTKFFTIGDLFRAMKWLGWGTMAYFIWCYIFLDPK
                     QYLDYSFVGFSDIKGGYRFKFGIDFIAFLSVYYTVKYIKQKDSVSMWLSAANLAYLFF
                     LHQGRSVMISVVATLFVFYMFEVNWQRAFRIFTSIGIGFFVIMGILYAAIPDRINDYV
                     IQYTSLATVVFTGNASGESSVDARLEEMAILFVYLQSYPIGWWIGIGKLSAESAVAGE
                     HTIATVPPGDIGILGSIMTYGILGSVLIYLQFLIAFRSVKFIRRYKDEPFMIASKYFL
                     IFCFLSSLAKGYLFMQPGSTAIFILVIYAYKLLEVQMDRQGLPETAVLKNSF"
     gene            4343..5338
                     /locus_tag="PLM90_02780"
     CDS             4343..5338
                     /locus_tag="PLM90_02780"
                     /inference="COORDINATES: protein motif:HMM:NF027586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4915 domain-containing protein"
                     /protein_id="HMZ88322.1"
                     /translation="MQLIHPDKRILVIGSLNANPADRAISRQDIRSMQVIDENGKIHP
                     VNFFNTWGELGMSSMCFHPNDPFVFYLASSSEVFKFMLKENRYEQLDIPNLTDIHEIS
                     MIGEQVWISNTKHDEIVSYNIRTNRVDQRINLSDFKSNTPEEGEGEADVEVVDKFHCN
                     LIFEGYEGDLYILVHHTSGRQLMKRIAQKFIKSQGNGGVVDITEKKRYPLNFKAPHSV
                     RRINNQYWVFDSGHFELKVFDKNWNFLRTMDTKGFGRGGDLDVERNVYYAGVSETRKR
                     YLGLFPGGEAVPNMVQVFNINDYKLAGSIKIPNIEQINNVYCLSKEVADKLLQLK"
     gene            5342..6301
                     /locus_tag="PLM90_02785"
     CDS             5342..6301
                     /locus_tag="PLM90_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMZ88323.1"
                     /translation="MTTGKTILPTFLVVGANKGGTTSIYHYLKQHPEVYLSPIKEPHY
                     FSKDIDVNQFKKEFAHNKLQDIEKYVQGDMKQEFHAAFVRSEEQYLKLFKNVTSQKAI
                     GELSTSYLYSTVAATEIKKLIPNCKIIICLRNPIERAYSHYRMNLWTGNSNEFDFHKA
                     LLEDYQHQPKVWGNAHLYTEIGMYYQQVKRYLDTFGAENVKVIFTEDLKKNSAQVIRD
                     LYTFIGVDPSFNPDTSTQYNEVYTPKYKNLTWFLNKSGIRPFMKRISPKFLKGAVVKL
                     FYKQKADKGQMPPAAKSFLLEKLGPDVHALESLLNKDLSGWLK"
     gene            6309..7349
                     /locus_tag="PLM90_02790"
     CDS             6309..7349
                     /locus_tag="PLM90_02790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ88324.1"
                     /translation="MRPKVCVVLVNYKTWKDTLECMESVLRSNYTNYQIVVVENGSGD
                     DSWEQMLKWARGENAIDITTQNPLFHLTHPPVHKPLLYFRASPEEAAHMRLGPLDGIM
                     PLLFIKSDENRGFAGGNNVGMRYALNSEFDYVWLLNNDTVIEPNAIEELVKYCEVKKE
                     KKQTIGIVGSKLMVYHQPQKMQGIGAIFNKYSGKSKIIGAQEIDKNQYDEQEISCNYV
                     IGAAMFVSRPFLLDVGLMSEEYFLYNEENDWSARAKLKDWEVGVAVKSKVYHKQGAST
                     GNSPKKSKWQIKALHYKYRGKIKLYKKYYRPQLPFLYLHLITRSFKYVSKGNFSEAKV
                     IYQAILNTKFES"
     gene            7346..8077
                     /locus_tag="PLM90_02795"
     CDS             7346..8077
                     /locus_tag="PLM90_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88325.1"
                     /translation="MIISHKYKYVFIELPQTASSAIARELKENYDGHEILFKHALYST
                     DFLKQAKPEEKQFTRVSGLRNPMDICVSNYFKFKTDHENRYSNPRLMKHGFLRKYIMR
                     WWNVRQYKSIIEGGENFENFFMRAYTLPYASWSIIEHKRMDYVIRFENLQADFAKALE
                     LIGVDKVRDIPVANKTAEKSKTFWEYYESDKAKRRAKYIFGPYMQRWGYDFPESWNHI
                     RIPWHSFFLYRIVNVARIIFWKFLR"
     gene            8084..9031
                     /locus_tag="PLM90_02800"
     CDS             8084..9031
                     /locus_tag="PLM90_02800"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMZ88326.1"
                     /translation="MAAKPTFLIVGAVKAGTTSIHEYLQMHPEVYMSPVKETNHFSDA
                     DMLFDHFNVDYKQDINANLEKYLAGPMDKKIHIAHVRTAEQYAALFKNVTSQKAIGEV
                     SNSYLYLPSTARAIHKALPDVQIVMILRNPVERLYSQFLMNLKLGKIVEKDLLKEIEA
                     DQVKPVKGWGVSHLYLEVGMYYEQVKRYLDIFDKNKVCILYYDDYKKDPTGTMQSLFK
                     FLGVDATFTPDMSKRYNEAGMPRFGKLNYWLTQIGVYGLVKRIFPSNLKERLKSLIYT
                     KENIPTITAEEKEYLINYYRKDIEQLQLLLNKDLSNWLK"
     gene            complement(9032..10471)
                     /locus_tag="PLM90_02805"
     CDS             complement(9032..10471)
                     /locus_tag="PLM90_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HMZ88327.1"
                     /translation="MAQENSERDLSARAVKILRWHEHLISFFQQYLNEKQFVTISGVL
                     IGITSALAAILLKAMVFYIHALLFTESNIGKISPYIYSSFPLLGILVTTWIVQRYFGG
                     NLGGGNAAIIFSIKKKESRLPGKLMYSHLITSAITVGTGGSTGLETPIVTTGAAIGSN
                     YGKTYKVGPEERTLMIASGVAAGIAATFNAPIAGVLFAVEVLISEITIVAFIPILIAA
                     ASGAILSKIILNEEILLNFSNMAPFNYHNVPFYILLGLLSGVIAVYYIRTSLRIERIF
                     RRVKQRFYVQRAIIGGILLGILILLFPPLFGEGYESIKTLAGNHAEDILKGSVVVGNL
                     QDPVMMIIVLTFVLLLKVVATSITLGSGGNGGNFAPSLFTGAYLGYIFAGLINLTGIF
                     NLPPGNFTVVAMAGMLSGIFHAPLTAIFLIAEITGGYDLMVPLMIVSAISFGVSRYLF
                     TYALDGLKLNSMLQQAAREEQEENKAKTE"
     gene            10597..11163
                     /locus_tag="PLM90_02810"
     CDS             10597..11163
                     /locus_tag="PLM90_02810"
                     /inference="COORDINATES: protein motif:HMM:NF011094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HMZ88328.1"
                     /translation="MATKIRLQRHGRKKAPFYHIVVADVRAPRDGKFIERLGSYNPTT
                     VPAQIIIDVDKAVDWLQKGAQPTTTVNAILRYKGVLYKKHLLRGVAKGAFTTEVADQK
                     FDEWVKSHETAVIDKVKAIEASAAATKAAEVARINAKRLEEASKKAAAEREAEAAATV
                     ETATEEAAVEETTSEAANDATTEETTNE"
     gene            11173..11679
                     /locus_tag="PLM90_02815"
     CDS             11173..11679
                     /locus_tag="PLM90_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88329.1"
                     /translation="MQGELVEIGYVIRTHGVKGHVRIAFDDNCKELSVSEALYFLIKG
                     VHMPHFISEISHFNNGDALVLLEELHNKEEADLYTKRPVFGPAEWLEDTDEDQEIAFE
                     GWKIHDETYGELGQIIASADMGAYLLLTVSHQGRELLVPMHDHLIIREDESSETLFTK
                     LPEGLTDL"
     gene            11691..12371
                     /gene="trmD"
                     /locus_tag="PLM90_02820"
     CDS             11691..12371
                     /gene="trmD"
                     /locus_tag="PLM90_02820"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMZ88330.1"
                     /translation="MRIDIITVLPELLDSPFQHSIMKRAADKGLLDVNLVQLRDYGLG
                     QHKQVDDYQFGGGAGMVMMVEPIFNCINALKAQRIYDEVIYLTPDGERFNQKIANQLS
                     LKGNIILLCGHYKGVDERIREHLITREISIGDYVLSGGELAAAILVDAIGRLIPGVLG
                     DETSALFDSFQDDLLAPPVYTRPANFQGWEIPEILLSGHTAKIEEWRHDEALKKTKSR
                     RPDLLPEN"
     gene            12435..12797
                     /gene="rplS"
                     /locus_tag="PLM90_02825"
     CDS             12435..12797
                     /gene="rplS"
                     /locus_tag="PLM90_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMZ88331.1"
                     /translation="MEFLQSLAQDLIPSKEIPQFKAGDTVTVNYKIIEGDKSRTQAFK
                     GDVIQRKGTGRTQTFTVRKISNGVGVERVFPLFSPNIESIEVNKSGKVRRAKIFYLRG
                     LKGKSARIKEVKSTSVAE"
     gene            complement(12884..13600)
                     /gene="bshB1"
                     /locus_tag="PLM90_02830"
     CDS             complement(12884..13600)
                     /gene="bshB1"
                     /locus_tag="PLM90_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HMZ88332.1"
                     /translation="MKLDILAFGAHPDDVELGCSGVLIKEVRNGKKVGIIDLTRGELG
                     SRGTAEIRAQEAADAAQIIGCSIRENLELPDGFFEDNNANRAIVIQAIRKYQPDIIIC
                     NAIHDRHPDHGRGSNLLESSAFLSGLIKIETTENNIAQKPWRPRLVLHYLQDRWITPD
                     VIVDISDVWEERMESIAAHRSQFFDPGSNEPETYISSKNFFEAIESRAREIGRPAQFK
                     YAEGFTCSRTIAVKSLGELM"
     gene            complement(13620..14135)
                     /locus_tag="PLM90_02835"
     CDS             complement(13620..14135)
                     /locus_tag="PLM90_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ88333.1"
                     /translation="MRSNTFPSIHALNVTALSGRTFSMSEYTGKVLLIVNTASKCGHT
                     YQLGLLEELYQEYKDRGLVVIGFPSDQFAQEPLNGEQIQEHCQVNYGVTFPMMAKSKV
                     RGDEANAVFKFFSSKSLNGKFSKQPRWNFYKFLVGRNGEALDHFWTYRKPNNAALRKA
                     IERALMATNPK"
     gene            complement(14193..15131)
                     /gene="trxB"
                     /locus_tag="PLM90_02840"
     CDS             complement(14193..15131)
                     /gene="trxB"
                     /locus_tag="PLM90_02840"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HMZ88334.1"
                     /translation="MHMERVKCLILGSGPAGYTAAIYASRANLQPVVYTGIEPGGQLM
                     QTTDVENFPGYPQGIMGPELMEDLKKQAERFGTDVRFGIATRVELNDRVKTVHFDDGT
                     SIEAYTVIISTGASAKWLGLPSEKRLNGNGVSACAVCDGFFFRNQEVALVGGGDTACE
                     EALYLSKLCTKVHMLVRKDSFRASKIMQERVLNAPNITVHWHSETLEVLGDQTVTGIR
                     IRNNKTNEETVINITGFFVAIGHQPNTQIFKDQIDLDEQEYIITKAGTTKTNIEGVFA
                     AGDVQDKHYRQAITAAGTGCMAALEAERYLTEKGIH"
     gene            15288..16826
                     /locus_tag="PLM90_02845"
     CDS             15288..16826
                     /locus_tag="PLM90_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88335.1"
                     /translation="MKLRYLPLMTLGLLIGSISAQNVDHPYAFGFGAHFIDYNIEDDG
                     YTKDLFDSADINGAWVANRYTFGGSINNSFSMEASLAAASISTISKGNIRETFLWCGV
                     DLGLRYQFANGYMLPHDSWFAPYLAGGVGANYLDSDDPDATTLFPEGEIAIGTNLWLT
                     PMFGINLQTGYTHQFSETGSDYMQHAVGLVIRFGKGEDKDGDGIANWEDSCPDKAGIP
                     LFNGCPDTDSDGITDAMDACPTENGLLQFNGCPDSDSDGLADKDDACPTEKGLAKFSG
                     CPDSDNDDIPDKDDRCPKDKGLKEFNGCPDSDGDGIADLDDACKQEKGLAKFGGCPDT
                     DGDGIMDKEDRCPRERGEIALKGCPDSDGDGVADIDDKCPDKPGIKATGGCPVIEEET
                     KKAIIEKINFAAKSIQFESGSDVIKKSSYPVLDQIVAIMILYPGTNWSIEGHTDDQGD
                     DKFNQELSDKRAAAVKNYFGSKGVADARLSSAGFGETKPIADNKTSAGRAQNRRVEIK
                     LVEQ"
     gene            complement(16882..17763)
                     /gene="era"
                     /locus_tag="PLM90_02850"
     CDS             complement(16882..17763)
                     /gene="era"
                     /locus_tag="PLM90_02850"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HMZ88336.1"
                     /translation="MSYRSGFVNIIGRPNVGKSTLFNVLIGEKLSIITPKAQTTRRRI
                     LGIVTTDQYQAVFSDTPGLIDQPEYKLHQWMNAQVSTALKDGDVLVFMTDPKDELHAD
                     HPLIQTLQQIRTPVIVVINKSDIIAKNVLEQLMIKWTSVLPKATIISISALQKINTDK
                     LFQHIVDALPVHPPYYDNDTWTDQTERVIAAEIVREKIFMQYQQEIPYNVEVIVDSYK
                     DKGDIIAISAVIYVGRDSHKSIIIGKSGSAIKNIGIAARQDIEKFAGKKIFLELFVKV
                     RENWKNDDRGLKHFGYE"
     gene            17873..17945
                     /locus_tag="PLM90_02855"
     tRNA            17873..17945
                     /locus_tag="PLM90_02855"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17906..17908,aa:Met,seq:cat)
     gene            18078..19391
                     /gene="der"
                     /locus_tag="PLM90_02860"
     CDS             18078..19391
                     /gene="der"
                     /locus_tag="PLM90_02860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMZ88337.1"
                     /translation="MGFTVAIIGRPNVGKSTLFNRLTGDRHAIVDDLSGVTRDRHYGM
                     VDWNGKIFDLIDTGGFVAHSSDVFEREIRRQVQIAIEEASLLLFMMDVTTGITDFDDD
                     VIKLLRRCGKPVFVIVNKVDNNRRHLDATEFYAAGFPDIFFLSSISGSGTGELLDAVA
                     ARIPGEDASELKELEALPRFAIVGQPNVGKSTLLNALTGQERTLVTDIAGTTRDAIHT
                     RYRLFDKDFVLVDTAGVRKKSVVKEDLEFYTVIRTIKAIDECDVCMLMIDATTGIEAQ
                     DLAILNMIEKKKKGVVILVNKWDLVAKETNTMLRMEEVIRERVAPFRDIPIIFISAVD
                     KTRIFKAMETGLEVYNSKNLKVSTSKLNEVMQEALEKYHPPAVRGNYIQIKYVTQMPA
                     QVPTFVFFTNYPNDIREPYRNYLENQLREHFPLSGVPINIFFRKK"
     gene            19443..19685
                     /locus_tag="PLM90_02865"
     CDS             19443..19685
                     /locus_tag="PLM90_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88338.1"
                     /translation="MKKVTYFAFALALTVLVSCKGKSTAEEGDTTTTETTTVETGASE
                     NVDNAAQVDEVLNQSSDDELFGTDVDDAAVEGATTK"
     gene            complement(19756..21354)
                     /locus_tag="PLM90_02870"
     CDS             complement(19756..21354)
                     /locus_tag="PLM90_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ88339.1"
                     /translation="MNKYRFLIPIGFAVALAVGVMIGVKISPFAGAAKVYGGASQNNE
                     RLNEILGYISQRYVDSVNVDSIMDAFVSDYLNDPATIEELFKQLDPHSSYIPKEDLQG
                     FNEDLDGNFDGIGIEFNIVDDTIMVVAAMSGGPSNKLGIQAGDKIISIDDSVVAGIGV
                     ASENVIKKLRGKKGTSVKVNIKRQGEKSLLPFTIIRDVIPVTSIDASFMLDAHTGFIR
                     VNKFAEQTPIEFKEALALLVNEGMQNLILDLRANPGGYLTGAVSIADELLPGTPLIVY
                     TNGRSVGRNDYYAGKNGLFEQGKVVVLINEYSASASEILAGALQDNKRATIVGRRSFG
                     KGLVQQVYDLPDSSAIRLTVARYYTPNGRCIQRPYDKGVDAYYDDYMEILMNGGEIPD
                     SLKSLKDVDWGIHPDISVPLDTTPENQTLNYLLNRSLIQQYCYSAYSANPGSFNKNKD
                     MLAFAKTYQITPEMFNKFMQFVHTHDTEKGVTDAEINAAKAKVEIAIKAYFARQLWGD
                     NGYYCTMSMIDDTLTKAINTIEKK"
     gene            21543..22508
                     /locus_tag="PLM90_02875"
     CDS             21543..22508
                     /locus_tag="PLM90_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HMZ88340.1"
                     /translation="MNEFIIAFRECLEAALIVGIIYVVLLKNNAKSQIKMVWLSVAAA
                     VTASAIMGVGLYEVLESAGDAFKEFLEGIFMYITAGLLFYVIFWMSKNLASREAITNQ
                     TKATLSQGSRWGIFFLVFFAIVREGFETALFLVASTGIDKSFSYIGFLTGVALAVLIG
                     YLIVVQGKRIQLKPFFKWTSLLLVVFAAGMIAYGTHEIHEYMEYREHEEHASAEATET
                     VNEECHELLEHAVGAESAESEAEEEEEYVFDIFKNKIGNEPLGGMYIYDEDCGVNVHM
                     LNANGLIGSFLKGLFGYNTSPTLMEIILWLLSLGFGIFIWRRAYK"
     gene            complement(22558..23169)
                     /locus_tag="PLM90_02880"
     CDS             complement(22558..23169)
                     /locus_tag="PLM90_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMZ88341.1"
                     /translation="MHITILGAGNIGLAAGKKWKDKGHSITFGVRDPESQKAWDARNI
                     GAEVKPFQEAMQGAEVVLIAVPFGVTDEVIKNIQFDWDNVIIIDTTNPISASTEPFDS
                     AAEAIAAWTGNGNVVKAFNTTGVGNMLNPIYNGTAIETFICGDDADAKKCVAGLAEDL
                     GFRVMDMGGLQNAVLLENLAKMWVTAAYKLGKGPNFAFTVVQR"
     gene            complement(23187..23600)
                     /locus_tag="PLM90_02885"
     CDS             complement(23187..23600)
                     /locus_tag="PLM90_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HMZ88342.1"
                     /translation="MKRNATAVWNGSGKEGAGTMSTQSGVLNNNQYSWSTRFADGIGT
                     NPEELVAAAHAGCFSMKLSFVLGAANLTPDKIETTCTITLDNGAITNSHLVVKATVPG
                     ATAESFAAAAAEAKANCPISKLLNTEITMEATLEG"
     gene            complement(23708..25912)
                     /locus_tag="PLM90_02890"
     CDS             complement(23708..25912)
                     /locus_tag="PLM90_02890"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMZ88343.1"
                     /translation="MIQRKLHLLALALVISVTSIVRPAFAAVNPDEGMWLANLVRQLN
                     FDYLRQMGLQLSAEEIYNTENASLKDAIVQLTQDGSGFCTAELVSENGLLFTNHHCGY
                     DAIASQSTTEHNYLDDGFWAKSYKEELPIPGLAIRILQDAIDVTDSIIPKVEGLDMAA
                     RRAKVTEIQNRMIAKYAEKGYEAELKSMYYGNQYFIYLYKSYNDVRLVGTPPSSIGKF
                     GGDTDNWMWPRHTGDFSIFRVYADKQNNAAEYSPENVPYKPKKFLTVSLDGYKEGDFT
                     MIMGYPGSTERYLTSFGMNEVITESNPAQIDVFKTVTDVMKQDMDKSEATRIQLASDY
                     ASLMNALKLWETQIDGMERMDAMQIKEDREKAFMKWAKTQGSAKEAMYKEMFENFKNT
                     YSTLSTVNKEFYYKIYSVVIVPSGSFALDFNDIETLFGEEKPSEEVKAATIAGIEASA
                     EEMWKTYNYETEVKKTAAILNMLYTKLPAEKYPQVLKDILAKSKGATPQEKFDNWSKA
                     AFAKSVFTTKEKLEAFLKSPSAKKLKKDPLYNYYIALYTDAQTAGTTFRASNTEINRL
                     EREYVAGILEQNAGKVFYPDANFTLRLTYGTVQDYIARDAVFYHWQTTLDGIMEKRDN
                     TNEEFVVPDKMYELWKNKDFGRYADKNGTVPVCFISNLDITGGNSGSPIMNGKGELIG
                     LAFDGNYEGTPGDYIFDPSMNRTISVDIRYVLFVIDKYAGASNLINELKIAR"
     gene            complement(26019..26405)
                     /locus_tag="PLM90_02895"
     CDS             complement(26019..26405)
                     /locus_tag="PLM90_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88344.1"
                     /translation="MRKLYTFMLLVSATVTGAFATLPSGDGEACVMEESTALSRAYGY
                     FGDDPTISIFPNPVKSYATISFTYENVDRICILNIVGREIKTIVPEAGKDEIKVSLVD
                     LQPGWYFLAAYSQGSTLITKKFLKEE"
     gene            complement(26551..28086)
                     /locus_tag="PLM90_02900"
     CDS             complement(26551..28086)
                     /locus_tag="PLM90_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ88345.1"
                     /translation="MQQQIKTLGELKASGYTAVSVKEEMRRNLIARLKSGKPAFEGIH
                     GFEHTVIPDLERAILSRHNIILLGLRGQAKTRIARLMTSLLDTYIPFVAGSELHDDPL
                     HPISFFARRRIEEMGDDTPIDWLHRDERYTEKLATPDVSIADLIGDVDPIKAANLKLH
                     YNDERVIHFGLIPHAHRGVFVINELPDLQARIQVALFNILQEGDIQIRGFKLRLPLDI
                     QFVFTANPEDYTNRGAIVTPLKDRIESQILTHYPKDIATSRIITSQEAQIHKEQAGVV
                     TLNNLARDLVEQIAFEARKSEYVDQKSGVSARMTITALENLVSAAERRALVNGESSTH
                     VRVGDFWGLVPSITGKIELVYEGEQEGPYIVALNLIGKAIRAQFANYFPEPEKAKKVG
                     NKKLAGNTDPGTGSMYQDIVNWFNDGNTLDILNGTSGRTHKANLQKIPGLKKIVERFH
                     GDKTEEEKLFLMEFLLHGLAEYSLLSKHFLQSGMQFTDLLSGMFSDDSFDALEQFGDP
                     DDQ"
     gene            complement(28108..29202)
                     /locus_tag="PLM90_02905"
     CDS             complement(28108..29202)
                     /locus_tag="PLM90_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ88346.1"
                     /translation="MLGNRFSKYEEKPQTTFDTLFELLQELLVYTSGDVTEALDWLNQ
                     LDRQYQITTPEYGMGDFIQELKDRGYLQDDEQQAGTMRITGKMEQSIRRSSLDQIFGK
                     LKKSQRGNHKTSQTGSGDENTTDSRPFVFGDTHEQIDYNESLKNAYINHGIAGFSMDE
                     SDLVVREREFRAQTSTVLMIDISHSMILYGEDRITPAKKVAMALSELINTKYPKDTLD
                     VIVFGNDAWQIEIKDIPYLQVGPYHTNTVAGLELAMDILRRRKNANKQIFMITDGKPT
                     CIMRNGKYYKNSFGLDRIIVNKTLNLAAHCRKLGIRITTFMIARDSYLQQFVEEFTEI
                     NQGKAFYTSLKGLGDYIFEDYERNRRRNVR"
     gene            complement(29215..30258)
                     /locus_tag="PLM90_02910"
     CDS             complement(29215..30258)
                     /locus_tag="PLM90_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ88347.1"
                     /translation="MMRYKLLGRSGLRVSELCLGTMTFGEEWGWGAGFEESSRQFHAF
                     LEAGGNFIDTANRYTEGSSERFLGELVKQSGRRDDLVVATKYALVTHERGLNLAGNHR
                     KNMVQSVEGSLKRLQMDHIDILYLHAWDFTTAAEEVMRALHDLVQQGKIFYVAISDTP
                     AWIVSRANTIAELRGWDPFIALQVEYSLIQRTVERDLLPMAQSMELAVTAWAPMAGGA
                     LTGKYLQENNDPKRLQPNSARLNERSRAIAQEVVNIAHETGHSAAQIALRWVMQQHQQ
                     IIPIVGARNADQLKDSIGATEIVLSQEHLQRLQQVSAIELGFPHDFLKGDGVRDILFG
                     GKLGELDIHKRFF"
     gene            30402..31796
                     /locus_tag="PLM90_02915"
     CDS             30402..31796
                     /locus_tag="PLM90_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMZ88348.1"
                     /translation="MRLRLLAALACITFTAFQQTANAQTAPAKTLDDKAAFNAIFDEV
                     MNNGEAYDNLEVLCKDIGHRLAGSPQADMAVRWSYDMIKKYGFDSVWLQPVTVPHWVR
                     GEQEYGEIMNVGKVNMLALGGSVATPIEGITDNVIEVRDFKHLEELGTAGIKGKIVFF
                     NYPFPQNVVNSFEGYGDAGPYRWHGANDASKYGAIAVVIRSVGSSHDDFAHTGSTHFD
                     DGVKKIPCAALSNIDADMLHNALQKDPSTKFKMIMSCAMLDSVQSYNVIAEIKGSTFP
                     KEVIVVGGHLDSWDVGEGAHDDGAGCMQSVEVLRAIQAAGLKPKRTIRCILFMNEENG
                     NMGGKTYGKYASATKDEKHIAALESDAGGFSPRGFSLDSAMLARPEMEQLKELMLKYG
                     VYSFEEGHGGTDIGPMEKAGTHLIGLVPDSQRYMDLHHTNNDVFEQVNKRELHMGASV
                     MAMMCWWISEHGVK"
BASE COUNT         9263 a         6387 c         6768 g         9471 t
ORIGIN      
        1 tatagtgttg catttcgaat tgtatttttt ttaggattgt ttttatcggc agctacattt
       61 ttcattgtac cttattttac ggataatctg tttgatacaa gttcacctaa ttacgatcag
      121 tatacattct tcatgcaaat atttgctgtc accattttat tttatgtgct cggcggtttg
      181 agtgaagaat gtattaaagg gctgaagaaa acgcgtcagt atagttggat ttctcaaaca
      241 atcaaccagg ttgcagccat cgcattttta ttgctttttg gccttttttc caaggatgaa
      301 ctttgggtga atttatctta ttgcctcggt gtatttgtag cctttgccat tgcccaatat
      361 tattggatat attatttgaa gcatccggag gtgggcggat cggaatttgg cagaaagaaa
      421 gacaaaaaag aaaagaaacc agcaaaagac aatgctgcag cggcagtatt agccgtcgcg
      481 cagcaagagc agccaataaa tacaggcgag atgctcaaat tgtcggcacc aatgatgatg
      541 gctaaatatc ttaccatgat ctatacatgg ttagatattt taatattggc atggtatgcc
      601 ccggaagcaa tggtatcgat ttatcgtgtt gctacacgat tgacttcctt atctactatt
      661 ccattggttt ctataaattc aatcgccgga ccaaaatttg ctgaagcctt tggtaaaaac
      721 gatcaacgca gtctaagaaa taacgttaag caggcaacgc gcatgatttt ttggacctgt
      781 atacccatac tggcgatatt tttgattttc ccgaaattca gcatgtggac ctttggaaaa
      841 gatttcacgg gcagtgaagc tgtaaccgtt tttgttatcg tttcactggg acaagctgca
      901 aatattctta caggtccggt gactgttctg ttgaatatga ccggacgcca aaatgttacg
      961 atgtattatg cattggcaac tgtggtaatt gatgtatcat tgaacttaat cctcatccct
     1021 atatacggta ttataggtgc tgcaattgct actgctgttg cgagaacagt attgaatgta
     1081 ggatgcgcac tgcagatata ttttacaatg ggcattagta ctatttataa tccattcgct
     1141 gatttatgga gtgtattttc caaaaagaaa atgcaacctg tgatcaacga gtcaaaaaac
     1201 gaggaggaaa tatgacgcaa cgttggccca attttctcat tgtaggtact gcaagagcag
     1261 gaacaacatc attgcatgaa tttttaggca atcatcctga tatcttcatg ccattgcaga
     1321 aggaaccatg tttctttact tatgtagatg agcagcctga atttaaggaa gcacgtcatc
     1381 ggtacctcac taatcctgct gaatatcttg ccatgtttga gcatcataat gaggccgtca
     1441 tgggagaatc ttcaacacca tatttatatt ttcatcaaaa atctattgcc aatatcaagc
     1501 gattggttgc gaatgcagat gagatgaaaa tactcattgt gttacgcgat ccttctgaga
     1561 gagcatattc gcaatatatg cataatcgcc gcgacttgag ggagccgctg acatttgaag
     1621 aggcaataga tgctgagaag caacgtatgg ctgataactg gcattttgat tttttctacg
     1681 ttgacaaggg gtattattat cagcaagtaa aagattatat agacaatttc aagcatgtta
     1741 aaatcgtgtt ttacgatacg cttgaaaagc aaccggaaaa gttgttgtcc gatatttatc
     1801 agtttcttgg cgttacacaa ttgcaagaag cgaaaatggt aaaacgcaac cagagtggtg
     1861 aaatgaaagt taagtggttg aagcaaatga tgacgacgag gaaaaatccg atacttaatt
     1921 tctttagaaa gttaatgagt cgcgatacaa aaaagaaatt aagaaattgg gctaaggata
     1981 aattgttgag atataatttg aagaaaacag aaatgaaccc tgagaccagg aggaagttaa
     2041 ttgcattgta ttctgaggat atcaagcaat tgcagacact tgttcagaag gaccttagtg
     2101 attggttaag agtataatat gagtgggatc aaacaaaatt ttatttgcat tggagcacaa
     2161 aaagccggca caactacctt gtccgacatt ttaagtgcgc atcccgatat ttgcatccct
     2221 cccattaagg agacgaagtt ttttttgttt gatcaggact atcagaaggg taagtctttc
     2281 tatgatcaat atttcagcaa ctacagaact caatctgctg taggtgaatt tgatcctgat
     2341 tatttgttgt ttccatatac tgccgaacgc attgctgaaa cgttgggaac ggatattaag
     2401 atattggtgg tattgcgaaa tcctgccgat agggcatatt ctcactacct gatgacaaaa
     2461 agaaagggtc ttgaacctca tgactttgag gccgctcttc aggctgaagc gagcaggaag
     2521 gaagatttga agaccagaaa aatctttgct tacattgaac ggggcatgta tggtactcag
     2581 atagcgcaat ttttaaaggt gtttccggct gagaattttt tgtttctggt atttgaggaa
     2641 gattttatca aaaacaggca agggacagtt gagaaggttc aggccttttt aggggttccc
     2701 ttcaaggcgc tggatgtcga tttgcatagc aatgaggcag gtgaacctat caatgattcc
     2761 ttgaatgagt tagttagaaa gcctaatttt gcaaaacgta tattaaagac aattttgcct
     2821 tttaaagcca taagaaaatg gatgagaaag gcatttatca gtcaaaatat gcaaaaagtt
     2881 agttctccta aactgagtga ggagcagagg agacaattga ttgccgatta ttttattcaa
     2941 gatattcatc tcacagaaaa attgacaggt cgcgatctgt cgctgtggta taattaatat
     3001 gggtatatta acatacgaaa aaggaattta taaggaacca atgtcagtct ttggtaagac
     3061 tatgatattg gtggtattta tcctttttac cacgcgtatc cttaccagaa cgcctttagt
     3121 ggattactat atgccattcg agattttgtg gtataccttc attattacgt tcacatttat
     3181 ttatgcgatg tatattttcc tggttcgaaa gaccgtggat gaaatacatt attattatat
     3241 attaattatt atcatgccct tgtgggcggg tttcatggcc aatttgagtg aaggtcagaa
     3301 tgtgatgggt ggcattagct cgcagcgaca ttggtatggg atttcaggga tatttgtatt
     3361 gatttatctt ctccaaacaa agttctttac aattggagac ctttttagag cgatgaagtg
     3421 gctgggttgg ggaactatgg catatttcat ttggtgttat atatttcttg atcccaaaca
     3481 atacctagat tattctttcg tagggttctc ggatatcaaa ggtggctatc gatttaaatt
     3541 tggtatagac tttatcgcat ttttatcagt ttactatacg gtaaagtata tcaaacaaaa
     3601 ggattctgtc agcatgtggt tatcagccgc taacctggca tacctgtttt ttctccacca
     3661 aggccgatcg gttatgattt cggtggtggc cactttattt gtattttata tgtttgaagt
     3721 gaactggcag cgcgccttta gaatatttac ttctattggt attggctttt ttgtaataat
     3781 gggtatttta tatgctgcaa ttcctgatcg catcaacgat tatgtaattc aatatacttc
     3841 tcttgctaca gttgtcttca cagggaatgc ttcgggcgaa agctctgttg atgcgcgttt
     3901 ggaagagatg gcaatcttgt ttgtttattt gcaaagctat ccaatcggtt ggtggatagg
     3961 cattggtaag ctaagtgccg agtcggcagt tgcgggcgag catacaatag ctactgtgcc
     4021 tccgggagac ataggtatac tcgggtctat tatgacgtat ggtatcttag gaagtgtttt
     4081 gatttatctt caatttttaa tagcattcag aagtgtaaaa tttattcgcc gttataaaga
     4141 tgagccattc atgattgcat cgaaatactt tttgatattt tgttttttat catcccttgc
     4201 aaaaggatac ttatttatgc agcccggctc aacagcaatt tttattcttg tgatttatgc
     4261 ttataaatta ttagaagttc aaatggacag gcaaggcctt cctgaaactg cagttttaaa
     4321 aaattctttc taaatttaga ttatgcaact tattcatccc gacaaaagaa ttttggttat
     4381 cggttcatta aatgcaaatc ctgctgatag agctatcagt cgccaggata tccgtagcat
     4441 gcaagtaatc gatgaaaatg gaaagataca tcctgtgaat ttctttaata cttggggtga
     4501 attaggtatg agcagtatgt gcttccatcc aaacgatcca tttgtttttt atctcgcttc
     4561 cagcagcgaa gtatttaaat tcatgctcaa ggaaaacaga tatgagcaat tggatattcc
     4621 aaacctcact gacatacatg agatttcaat gataggagag caagtttgga tatcaaacac
     4681 taagcatgat gaaatcgtta gctataacat ccgcacaaat agggtagacc aacgaattaa
     4741 cctttccgac tttaaatcaa atacaccgga agagggtgaa ggtgaggcag atgtggaagt
     4801 ggttgataaa tttcattgta atttgatatt tgaaggatac gaaggcgatt tatatattct
     4861 cgttcatcat acatccggcc gtcaattgat gaagcgaata gcgcaaaaat ttattaaaag
     4921 tcagggaaat ggcggagtgg tagatatcac cgaaaaaaaa cgctatccat taaatttcaa
     4981 agctccccac agtgtaagaa gaatcaataa tcaatattgg gtgttcgaca gcggacattt
     5041 tgaattgaaa gtatttgata agaactggaa tttccttcgc actatggata ccaagggttt
     5101 tggccgcggc ggtgatctgg atgttgaacg taatgtgtat tatgcaggcg taagtgaaac
     5161 ccgtaaacga tatctcggac ttttccccgg cggtgaagct gtcccgaata tggttcaggt
     5221 ttttaatatt aatgattata aactcgcagg gtctatcaaa attccgaaca tcgagcagat
     5281 caataatgta tattgcctat cgaaggaagt tgccgataaa ttgttgcagt tgaaataatt
     5341 catgaccacc ggcaaaacca tattacccac atttttagta gttggcgcaa ataaaggcgg
     5401 cacaacatca atatatcatt acctgaagca gcatcctgaa gtttatttgt cgccgataaa
     5461 ggaaccacac tactttagta aggatattga tgtaaaccaa ttcaaaaagg aatttgcaca
     5521 taataaactt caggatattg aaaaatacgt gcagggtgat atgaagcagg aattccatgc
     5581 tgcatttgtg cgaagtgaag aacaatattt gaagctattc aaaaatgtta catcgcaaaa
     5641 ggcaattggc gaattaagta cttcctattt gtattcaaca gttgccgcaa cagagatcaa
     5701 aaagctgatt ccaaactgta aaatcattat ttgcttgcgc aatccaattg aacgcgcata
     5761 ttctcattac aggatgaatt tatggacggg caatagcaat gaatttgatt tccataaggc
     5821 attgcttgaa gattatcagc accagcccaa agtatgggga aatgcccatt tatatactga
     5881 aatcggcatg tattatcagc aagtaaaacg ctacctggat acttttggtg ccgagaatgt
     5941 taaagtgata tttactgaag acctcaaaaa aaatagtgct caggtaatac gtgacctcta
     6001 cacatttatt ggtgttgatc catcttttaa tccggatact tcaacccaat ataatgaggt
     6061 gtatacgcca aagtataaga atctgacctg gttcctgaat aaatcaggta tcagaccatt
     6121 tatgaagcgc atctcaccta aattcctaaa gggtgcagtt gtgaaattat tctataaaca
     6181 gaaagctgat aagggtcaaa tgccaccggc agcaaaatct tttttgttag agaagcttgg
     6241 acctgatgta catgcacttg aatccttgtt gaataaagac ctttcaggtt ggttaaaata
     6301 atatccctat gcgacctaaa gtttgtgttg ttctggtaaa ttataagacg tggaaagata
     6361 cactcgaatg tatggagagt gtactgcgca gtaactacac aaattatcaa atcgttgtag
     6421 tagaaaatgg tagcggcgat gatagctggg aacaaatgtt gaaatgggct cggggtgaaa
     6481 atgctattga tattaccact caaaaccccc tttttcatct cacacatccc cccgttcata
     6541 aaccactgct gtactttaga gcttcacctg aagaggctgc gcacatgcgt ttaggaccac
     6601 tggatggcat catgccattg ttgtttatta aaagtgatga aaacagaggt tttgcaggcg
     6661 gtaataatgt tggtatgcgg tatgcgctta atagcgagtt tgattatgtg tggttgctga
     6721 ataatgatac cgttatagaa ccaaatgcaa ttgaagagtt ggtgaaatat tgcgaggtaa
     6781 aaaaagagaa aaagcaaact atcggtattg ttggcagcaa actcatggtg tatcaccaac
     6841 cacagaaaat gcagggtatc ggtgcgatat tcaataaata ttcaggaaag agtaagatca
     6901 taggtgcaca ggaaattgat aagaatcaat atgatgagca ggaaataagt tgcaattatg
     6961 tgataggtgc tgcaatgttt gtgagtagac cgtttttatt ggatgttgga ttgatgagcg
     7021 aggagtattt tctttataac gaggaaaatg actggagcgc acgtgctaaa ttgaaagact
     7081 gggaagttgg tgttgcggta aaaagcaagg tgtatcataa acaaggtgca tctacaggaa
     7141 atagccctaa gaaaagtaag tggcagatta aagcgctgca ctataagtat cgagggaaaa
     7201 ttaaattata taagaagtat tatcgcccgc aattgccctt tttatacttg catcttatca
     7261 caagaagttt taaatatgta agcaaaggaa acttcagcga ggcaaaagtt atttatcaag
     7321 ctatcttaaa cacaaagttt gaatcatgat cattagtcac aagtataagt acgttttcat
     7381 cgaactccct caaactgcaa gcagtgctat tgcccgtgag ttgaaggaaa attatgatgg
     7441 gcatgaaata ttattcaaac atgcactgta tagcacagat tttttgaagc aggcaaaacc
     7501 tgaagagaag cagtttacgc gcgtcagtgg gttgaggaac ccaatggata tttgtgtgag
     7561 caattatttt aagttcaaga cagatcatga aaacagatat tccaatcctc gcctgatgaa
     7621 gcacggtttt ctgaggaagt atattatgcg gtggtggaat gtacgtcagt ataaatctat
     7681 cattgagggt ggagagaatt ttgagaattt cttcatgcgt gcctatactt taccttatgc
     7741 cagttggagt atcattgaac ataaacgcat ggattatgtc atcaggttcg agaacttaca
     7801 agcagatttt gcaaaagcac ttgaattgat aggtgtagac aaagtacgtg atattcctgt
     7861 tgccaataaa actgccgaga agtcgaaaac attttgggaa tattacgaaa gtgataaagc
     7921 taagcgtcgt gctaaatata ttttcggccc atatatgcaa cgatggggct acgactttcc
     7981 tgagagttgg aatcatattc gtattccatg gcattccttt ttcctataca gaattgtgaa
     8041 cgtagcgaga atcatattct ggaaattctt acgttaatat caaatggcag cgaaacctac
     8101 attccttatt gttggcgctg taaaggcagg tactacttct atccatgaat acctgcaaat
     8161 gcatccggaa gtgtatatgt cgccggttaa ggagacaaat catttcagtg atgccgatat
     8221 gctgtttgat cacttcaatg ttgattacaa gcaggatatc aatgcgaatc tggaaaaata
     8281 tctggccggc cctatggata agaaaatcca tattgcgcat gtcagaactg cagagcaata
     8341 tgctgctttg tttaaaaatg tcacctctca aaaagcaata ggagaggtga gtaattctta
     8401 tttgtatttg ccttctacag ctagggcaat tcacaaagct ttgccggatg tgcaaattgt
     8461 gatgatatta cgtaatcctg ttgagcgatt gtattcacaa tttctcatga atctgaaact
     8521 tggaaaaata gtggaaaagg atttgctgaa ggaaattgaa gcagaccagg taaaacctgt
     8581 gaagggatgg ggtgtaagtc atttatatct ggaggtgggc atgtattatg agcaggtaaa
     8641 acgctattta gatatttttg acaagaataa agtttgcata ttgtattacg acgattacaa
     8701 gaaagatcct acaggcacta tgcaatcgct gtttaagttt ttaggtgtag atgcaacatt
     8761 cacacccgac atgtcaaaaa gatataatga ggcgggaatg cccaggtttg gtaaactaaa
     8821 ttattggctt acacaaattg gtgtgtatgg tttagtgaag agaatatttc cttccaattt
     8881 aaaagaaaga cttaaatcgc ttatttatac caaagaaaat attcctacta ttactgccga
     8941 agaaaaggaa tatctaataa attattacag gaaggatatt gagcaattgc agttgctgct
     9001 gaacaaagat ctaagtaatt ggttgaaata attattcggt tttagcttta ttttcttctt
     9061 gttcctctct tgcagcctgt tgaagcatac tatttaattt tagaccatca agtgcataag
     9121 taaacaaata ccttgaaact ccaaagctga tagcactaac tatcatcaaa ggcaccatca
     9181 agtcatagcc tcctgttatt tcagcaataa ggaatattgc agtgagtggc gcatggaaaa
     9241 tgccactgag cattcctgcc attgcaacaa ctgtaaagtt gcccggaggc aagttgaaaa
     9301 ttcccgtaag atttatcagt cccgcaaaaa tatatcccaa ataggcaccg gtgaataatg
     9361 aaggagcaaa attgccgcca ttgccaccgc ttcctaatgt aatggaagtg gccaccactt
     9421 taagtaataa tacgaaagtg agtacaataa tcatcatcac aggatcctgc aggttgccaa
     9481 caactactga accttttaat atgtcttccg catgattgcc tgctaaagtt ttgatgctct
     9541 catagccttc gccgaataat ggtggaaata ataatatcaa tatacccaat aatattccgc
     9601 cgatgattgc tcgttgaaca tagaaccgtt gtttaactct gcgaaatatt ctttctatac
     9661 gcagcgaggt tctgatatag taaactgcaa ttactcccga taacaatcct aataatatat
     9721 aaaacggcac attgtgataa ttgaaaggtg ccatgttaga aaagttcagg agtatttcct
     9781 catttaaaat gatcttcgac agaatggcac cggaagctgc agcaatgaga atagggataa
     9841 atgctactat ggtgatttca cttatcagta cttcaactgc aaacaacaca ccagcaatcg
     9901 gtgcattaaa cgttgccgca attccggctg caacacctga agcaatcatt aaggtccttt
     9961 cctccggtcc tactttatat gttttaccat aattggatcc tatggcagca cctgtagtca
    10021 caatcggtgt ctctaatcct gtagagccac cggtcccaac agtaatggcg ctggtaatta
    10081 aatgtgagta cattaatttt cccggcaggc ggctttcctt ctttttgata gaaaaaataa
    10141 tggccgcatt gccaccacct aggttcccgc caaaataccg ctgaactatc caagttgtaa
    10201 ctaatatacc taataaggga aatgaactat agatataagg tgagatctta ccgatattgc
    10261 tttctgtgaa caggagggca tgaatgtaga acaccatggc cttaagcaaa attgcagcca
    10321 aggcagaggt gatgcctatt aataccccgg aaatggtgac aaactgcttc tcgtttaaat
    10381 actgctggaa aaaggagatc aggtgctcat gccagcgaag gattttaaca gcccttgccg
    10441 acaaatcacg ctctgagttt tcctgcgcca tagggcgaaa ataggcattt cacttgtaaa
    10501 aaccacccga ttaaggtatc tttgcacccc aatctcaact ctccggctgg tccgggtcga
    10561 tctgagacat tttttaacta aaaagcatgt tttaacatgg caactaaaat cagattacaa
    10621 cgtcacggta gaaagaaagc tcctttctat cacattgtgg tagccgacgt aagggctcca
    10681 cgcgatggta aattcatcga acgcctgggt tcttataacc cgacaacagt tcctgctcaa
    10741 atcattattg atgtagacaa ggctgttgac tggctgcaaa aaggtgcgca acctacaact
    10801 actgttaatg ctatccttcg ctacaaaggg gtattgtaca aaaaacacct ccttcgtggt
    10861 gtggcaaaag gtgccttcac tactgaagta gcagatcaga aatttgatga gtgggtaaaa
    10921 agccacgaaa cagctgttat cgataaggtt aaagctatcg aggcatctgc tgctgcaaca
    10981 aaagctgctg aagtagcacg tatcaatgca aaacgtttgg aagaagcttc taaaaaagcg
    11041 gccgctgaac gcgaagcaga agctgctgct accgtagaaa ctgcaactga agaggctgcg
    11101 gtggaagaaa caacttcaga agcagctaat gatgctacta ctgaggagac aactaacgaa
    11161 taatcaaatc ccgtgcaagg cgaactggtc gaaatcggtt atgtgatcag aacgcatggt
    11221 gtaaagggac atgtgcgtat agcatttgat gataattgta aagagctttc ggtttcggaa
    11281 gctctttact ttttaattaa aggagtgcat atgcctcact tcatcagcga gatcagccat
    11341 tttaataatg gcgatgccct tgtattgctc gaagaattgc acaataagga agaagctgat
    11401 ctctacacaa aacgtccagt ttttggtcct gcagagtggc ttgaagatac tgatgaagac
    11461 caggaaatag cctttgaagg atggaaaatt catgatgaaa cctatggcga acttggtcaa
    11521 atcattgcct ctgccgatat gggggcgtat ctattgctaa cagttagcca tcagggtcgt
    11581 gaactgttgg tgcccatgca tgaccacctc attattcgag aagatgaatc ctctgaaacc
    11641 ttgtttacta agcttccgga gggacttacg gacctttaat tcataaccct atgcgcatcg
    11701 acattattac ggtattaccc gaattgctgg attctccgtt tcaacatagt atcatgaaac
    11761 gtgctgccga caagggtttg ctagacgtta acctggtgca gttgcgcgat tacggccttg
    11821 gacagcataa gcaagtcgac gattatcaat ttggtggtgg tgccggtatg gtgatgatgg
    11881 tggagcccat cttcaattgc atcaacgcct tgaaagccca acgtatctat gatgaggtga
    11941 tttatctaac gcctgacgga gagcgattta accaaaagat agccaatcag ctgtcgctga
    12001 aaggcaatat cattctgctt tgcggacatt ataagggggt ggatgaacgc attcgcgagc
    12061 acctgattac cagagagatt tctatcggcg actatgtact cagcggaggt gaactggctg
    12121 cggcgatact tgtagatgca attggcaggc tgattccggg tgttttgggt gatgaaacct
    12181 ctgctttgtt tgatagcttt caggacgacc ttttggctcc tccggtgtat acccgtccgg
    12241 caaactttca gggttgggag ataccggaaa tcctgctaag cggacatacg gccaaaatcg
    12301 aggaatggag acatgatgag gctctgaaaa agaccaaatc ccgccgtccg gacctccttc
    12361 cggaaaacta agccggaaag ttggcaaaaa cactaaaacc tgttatcttt gcagtccact
    12421 ttaaaaatta gtgtatggaa ttcttacaat cactcgcaca agaccttatc ccatcaaagg
    12481 aaatcccgca gtttaaagca ggtgatactg ttactgttaa ctataaaatc attgaaggtg
    12541 ataagtcaag aacacaggct ttcaaaggcg atgtgattca gcgcaaaggc accggtcgta
    12601 cgcaaacatt cactgttaga aaaatttcta atggcgttgg tgttgaaagg gtattccctt
    12661 tattttcacc aaacatcgaa agcatcgagg taaataaatc aggtaaagta cgtcgtgcta
    12721 agatcttcta tctccgcgga ctgaaaggaa aatcagctcg tatcaaagaa gtaaaatcaa
    12781 cttctgtcgc agaataaagg actgtcatga atatatagtt tgaataaccc tggtctaacg
    12841 atcagggttt tttgttttag agcaaactaa cactgaaaaa taatcacatc aattcgccga
    12901 gcgacttcac agcaatggtg cggctgcaag taaagccttc agcatatttg aattgtgccg
    12961 gcctcccgat ttcacgtgcg cgactttcaa tcgcttcgaa gaaatttttt gaagagatat
    13021 acgtttccgg ctcattactt cccggatcga aaaattgaga acggtgtgca gcgatgcttt
    13081 ccatacgctc ttcccataca tccgaaatat ctacaattac atccggtgtt atccatctat
    13141 cctgcagata atgcaatacc aatcgcggac gccagggttt ttgtgcgata ttattttcgg
    13201 ttgtttcgat tttaattaat ccggataaaa atgcggagga ctctaataag ttggagcctc
    13261 tgccatgatc aggatgacga tcgtgaatgg cattgcagat aataatatcg ggttgatatt
    13321 ttcttatcgc ctgaataaca atagccctat tagcattatt atcctcaaag aaaccatccg
    13381 gaagttccag attttcacga atagaacaac caatgatctg agcagcatcg gcagcttctt
    13441 gtgcgcggat ctctgctgtg cccctagatc ccagttcgcc acgtgtaagg tctatgatgc
    13501 ctactttttt cccgttgcgc acttccttta tcaatactcc gctacatcct aattcaacgt
    13561 cgtcaggatg cgcaccaaag gcaagaatat ccagtttcat cagacaaaaa taaccgagtt
    13621 tacttaggat ttgtagccat caaggctctt tctatcgctt tgcggagcgc agcgttattc
    13681 ggtttgcgat aagtccagaa atgatccagt gcttcaccat tcctgccaac aagaaattta
    13741 tagaaattcc aacgcggttg tttactgaat tttccattca gcgatttact cgaaaagaat
    13801 ttaaaaacag cattcgcctc atcgccacgg acctttgatt ttgccatcat cggaaaggta
    13861 acaccataat tcacctgaca atgttcttgt atctgctcac cgtttaatgg ctcttgtgca
    13921 aattgatctg atgggaaacc tattactacc aatccacggt ctttgtattc ctgatacaat
    13981 tcttccaata atccgagttg ataggtatga ccacatttac tggcagtatt tacgatcagc
    14041 agcaccttgc cggtatactc actcatagaa aaagtacgac cggataatgc ggttacattc
    14101 agggcatgta tagaaggaaa ggtgttactt ctcatatgct taactgaaca gcaaatggag
    14161 tgaaaagttc atcaccaaaa agtgacgatt tattaatgta ttcctttttc agtcagatat
    14221 cgctcggctt ctaatgcagc catacagccc gttccggcag cagttatagc ctgacgatag
    14281 tgtttatctt gtacatcgcc tgcggcaaaa accccttcaa tatttgtttt ggtggtgccg
    14341 gcttttgtta tgatatattc ctgttcatca agatcaatct gatctttaaa tatttgtgta
    14401 tttggctgat gaccaatggc aacaaaaaaa ccggtgatat tgataaccgt ttcttcattc
    14461 gttttgttgt tgcgaatgcg aattccggta acagtttgat cgcctaatac ttcgagcgtt
    14521 tctgaatgcc aatgcaccgt aatatttgga gcattcagta cgcgttcctg catgatttta
    14581 cttgctctga aactatcctt gcgaacaagc atatgtactt tagtacataa tttagataag
    14641 tagagagcct cctcgcatgc agtatcgcct ccaccaacca atgcaacttc ctgattgcgg
    14701 aaaaagaagc catcacacac tgcacaggca ctcacgccat ttccgttcaa acgtttttct
    14761 gaaggtaaac ccagccattt tgccgaggca ccggtagaaa taattacagt atatgcttca
    14821 atagatgtgc cgtcatcaaa atgaaccgtt tttacacggt cgtttaattc aacccttgta
    14881 gcaatgccaa atcgcacatc agtgccaaat ctttcagctt gtttttttaa gtcctccatt
    14941 aattcaggac ccatgattcc ctgcgggtaa ccgggaaaat tctcaacatc cgttgtttgc
    15001 attaactgac ctccgggttc gatgcctgta taaactacag gttgaagatt agctcgtgaa
    15061 gcataaattg cagctgtata tcctgctggt cctgatccta aaatgaggca ttttactctt
    15121 tccatgtgca ttgataaata gggtgtaaag gtaagcaaca aggcgctatt tctcaggttt
    15181 actaaacacc gggaaaagtg aaaaggtgca taagttgtgg ttaatattct atgtgtttac
    15241 aatgcatatt tgttaattag gggtttcttg accttaaaac ccagtctatg aagcttcgtt
    15301 acttgccctt aatgacgttg ggactgttaa ttggcagcat ctcagcgcaa aatgtagacc
    15361 atccttatgc ctttggattt ggtgcacatt ttattgatta caatattgaa gatgacggtt
    15421 atacgaaaga ccttttcgat agtgccgaca tcaacggtgc atgggtagca aatcgataca
    15481 cgtttggcgg ttccatcaat aattcttttt ctatggaagc atcgttggca gctgcatcga
    15541 tttcaaccat ctctaaagga aatattcgcg aaacattttt atggtgtggt gttgatttgg
    15601 gattgcggta tcaatttgca aacggataca tgcttccgca cgatagttgg tttgcgccct
    15661 atcttgccgg cggtgtaggt gctaactatc tcgattctga tgatcctgat gccactacat
    15721 tatttcccga aggcgaaatt gccatcggca caaatttatg gctcactccc atgtttggta
    15781 tcaatttaca aacaggctat acgcatcaat tcagtgaaac aggttctgat tatatgcaac
    15841 atgcagtcgg tttggtaatt cgctttggaa aaggtgaaga taaagatggt gatggcatcg
    15901 caaattggga agatagttgt ccggataaag caggtattcc gcttttcaac ggatgcccgg
    15961 ataccgatag tgatggcatc actgatgcga tggatgcctg tccaacagaa aatggattat
    16021 tgcaattcaa tggttgcccc gattccgata gcgatggatt ggctgataaa gacgatgcct
    16081 gccctacaga aaaaggatta gctaaattca gcggttgccc tgacagtgat aatgatgata
    16141 ttccggataa agacgaccgt tgtccaaaag acaaaggcct gaaagaattc aatggatgcc
    16201 ccgattctga tggcgatggc attgctgatc ttgatgatgc ctgtaagcag gaaaaaggcc
    16261 ttgcgaaatt tggcggatgt cctgatactg atggtgatgg catcatggat aaagaagaca
    16321 gatgtcctcg cgaacgtgga gaaattgcat tgaaaggatg tcccgattcc gatggcgatg
    16381 gcgtagcgga tatagatgat aaatgtccgg ataaaccggg aataaaagct acgggaggtt
    16441 gtcctgttat tgaggaagaa acgaaaaaag cgatcattga aaaaatcaac ttcgcagcaa
    16501 aatcaattca atttgaatcc ggaagtgatg taatcaaaaa atcatcctat cctgttttgg
    16561 atcagatagt tgctattatg attttatatc ccggaacaaa ctggagcatc gaaggacata
    16621 ctgatgatca gggtgatgat aaattcaatc aggaactgag cgacaagcgt gcagcggctg
    16681 tgaaaaatta ttttggcagc aaaggtgttg ccgatgcgag attgagttct gccggatttg
    16741 gcgagacaaa acctattgcc gataataaaa catcagccgg acgtgcgcaa aaccgacgcg
    16801 tggaaattaa attggtggaa caataaatat tcaataattt aatcactata aatgcctgct
    16861 tattttgagc gggcattttt tttattcgta gccgaaatgt tttagccctc tatcgtcatt
    16921 tttccagttc tcacgaactt ttacaaacaa ttcaagaaat atttttttgc ctgcaaattt
    16981 ttcaatatcc tgtctcgcag ctatgccaat attttttatt gctgatccgg attttccaat
    17041 aatgatggat ttgtgactat cacgtccgac atagattaca gcactaatgg caataatatc
    17101 acctttgtct ttataactgt caactatcac ttctacatta tacggtattt cctgctgata
    17161 ttgcatgaat attttttcac gaactatctc tgcagcaatc acacgttcgg tctggtcggt
    17221 ccaggtgtcg ttgtcgtaat atggcggatg caccggcaat gcatctacta tatgttgaaa
    17281 taatttgtcg gtattaattt tttgtaatgc tgaaatggaa ataatggttg cttttggcag
    17341 cacagaagtc catttaatca ttaattgctc cagcacattt tttgcaatga tgtcgctttt
    17401 attaatgact acaattaccg gtgtgcgaat ttgttgtagg gtttgaataa gcggatgatc
    17461 ggcatggagt tcatctttcg gatcggtcat aaataccaat acgtcgccat cttttaaggc
    17521 ggtacttacc tgcgcattca tccattgatg cagtttatat tccggttgat caattaagcc
    17581 cggtgtatct gaaaataccg cctgatattg atctgtagta acaataccca gtattcttct
    17641 tcgtgtagtt tgtgcttttg gtgtgatgat agacagcttt tcaccaatca atacattaaa
    17701 caaggtcgat tttccaacat ttggtctccc aatgatattc acgaagccgg agcgataact
    17761 catggcgcaa aattacggtt ttggagagcc aatcgctgat aatattaggt agaatggcaa
    17821 aaacaacgta tctttgcacc tcgttctgaa agaaatttgc ttcaagatat atcgcggggt
    17881 ggagcagttg gtagctcgtc gggctcataa cccgaaggcc acaggttcga gtcctgtccc
    17941 cgctacacag aaacccggta tctccgggtt tttttatatc actaactgaa gcaactgatt
    18001 tcaaacctgt caatgcaatg agggcgctgc actgatagcc ttaaatcgcc cgcaatacaa
    18061 aataatcacc cgatctcatg ggatttaccg ttgccatcat aggaagacca aacgtaggga
    18121 aatcgacgct gtttaacagg ttgacgggcg acaggcatgc catagtggat gaccttagtg
    18181 gggttacccg cgacaggcat tatggcatgg ttgactggaa cgggaagatt ttcgacctga
    18241 tagatacagg aggttttgtt gcacatagca gtgatgtgtt tgaacgtgag atccgccgtc
    18301 aggtgcagat tgccatagaa gaggcatcgc ttttgttgtt tatgatggat gtgacaaccg
    18361 gcatcaccga ctttgatgat gatgtaatta aattattgcg tcgctgcggg aagcctgtct
    18421 tcgtgatcgt gaataaggta gataataatc gccgacatct cgatgccact gagttctatg
    18481 ctgccggctt tccggacata tttttccttt catctatctc aggaagtggc accggagagt
    18541 tgctcgatgc cgtagcagcg cgcattccgg gtgaagatgc cagtgagctc aaagaactgg
    18601 aagcactgcc acgcttcgct attgtaggtc agccaaatgt cgggaaatcg acactattaa
    18661 atgcacttac cgggcaggaa cgcacattgg tgaccgatat tgccggaacc accagagatg
    18721 ccatccacac ccgatataga ctgtttgata aggattttgt gttggtagat actgcaggtg
    18781 tgcgcaaaaa atctgtggta aaagaggacc tggagttcta tactgttatc agaacgatta
    18841 aggcgattga tgaatgtgat gtgtgcatgt tgatgattga tgccactacc ggaattgaag
    18901 cacaagacct tgccatcctc aatatgattg agaaaaagaa aaaaggtgtg gtcattttgg
    18961 tcaataaatg ggaccttgtt gccaaggaaa ccaataccat gctgagaatg gaagaggtca
    19021 tccgcgagcg ggtagcgcca ttccgcgata tccctatcat ttttatatcg gcagttgaca
    19081 aaacacgaat tttcaaagcc atggaaacag ggctggaagt atacaacagc aagaatctga
    19141 aggtttctac cagcaagctg aatgaagtaa tgcaggaggc tttagaaaag tatcatccgc
    19201 ctgcagtcag aggtaactat atccagatca agtatgttac gcaaatgcct gcccaggtgc
    19261 caaccttcgt gttctttacc aattacccga acgatatccg cgaaccatat cgcaactacc
    19321 tcgaaaatca gttaagggag cacttcccgc tgtcgggggt gccgatcaat atttttttcc
    19381 ggaagaagta agggaaaaga aaattgctaa ttttgtatct ataataacca aacacaactg
    19441 tcatgaaaaa agtaacgtat ttcgctttcg ccctagcact cacagtactc gtttcttgca
    19501 aaggaaaatc aactgctgaa gaaggagata ccacaacaac tgaaacaaca actgttgaaa
    19561 ccggcgcttc tgaaaatgta gacaatgccg ctcaggttga tgaggtgttg aaccaatcat
    19621 cagatgatga actgttcgga acagatgttg atgatgctgc cgtggaagga gctacaacaa
    19681 aataatctga tctttgatca aacaaaaaag cccgtcggtt gtaactgacg ggctttttta
    19741 gatccatgat tgaaatcatt tcttctcaat cgtattaatt gcttttgtca aggtgtcatc
    19801 tatcatcgac atagtgcaat agtatccatt atcgccccaa agctgtctgg caaaatatgc
    19861 cttgatcgct atttctactt ttgctttagc cgcatttatt tcggcatctg taactccttt
    19921 ttcagtatca tgggtatgta caaattgcat aaacttgtta aacatctcag gcgtaatctg
    19981 atatgtttta gcaaacgcaa gcatatcttt attcttattg aatgatcccg gattggcaga
    20041 atatgccgaa tagcaatatt gctgaatgag tgaacggttc aataaataat tcaaggtctg
    20101 attttcaggt gtagtgtcca atggaactga aatatcagga tgtattcccc aatctacatc
    20161 tttcaaactt tttagactgt cgggaatttc tccgccattc atcaatatct ccatgtagtc
    20221 gtcgtaatag gcatcaacac ctttatcgta cgggcgttga atacagcgac cattaggtgt
    20281 ataatatcga gctaccgtaa gacgtatcgc cgagctgtcg ggaagatcgt aaacttgctg
    20341 tacgagccct tttccaaagc ttctgcgacc tacaattgtt gcgcgtttgt tgtcttgcaa
    20401 tgcacctgca agaatttcac ttgctgatgc agaatattca ttaatcaata cgactacctt
    20461 accttgttcg aacaatccgt ttttgccggc ataataatca ttgcgtccaa cactgcgtcc
    20521 gttcgtatac acaataagag gtgttcccgg caataattca tcggcaatag aaacggcgcc
    20581 tgtcagatag cctcccggat ttgcacgaag atcaaggatg aggttttgca tgccttcgtt
    20641 taccaataat gctaatgctt ctttgaattc aattggtgtc tgctcggcaa atttatttac
    20701 acgaataaat ccggtatgtg catctaacat aaatgaagca tcaatacttg taaccggaat
    20761 tacatctcgt atgattgtaa acggcaataa acttttttca ccctgacgtt tgatattgac
    20821 tttaaccgaa gtgccttttt tcccacgtaa cttcttaatg acattttcgc tggcaacacc
    20881 aattcctgcc acaacagaat catcgatgga aataatttta tcaccggctt gtatacctaa
    20941 tttattcgat ggtccaccgc tcattgctgc aaccaccata atggtatcgt caacaatatt
    21001 gaattctatt ccaatgccat caaaattccc gtcaaggtct tcattgaaac cttgcagatc
    21061 ttcttttgga atataactgc tgtgtggatc caattgcttg aataattctt caatggtagc
    21121 cgggtcattt aaatagtctg aaacaaatgc atccatgatg gaatcaacat tcacggaatc
    21181 gacatatcgc tgactgatgt atcccaatat ttcattgagt cgttcattat tctgtgatgc
    21241 acctccatat actttggcag caccagcaaa cggcgatatc ttcacgccga tcataacgcc
    21301 caccgccagc gcaacagcaa aaccgatggg tatgagaaat ctgtatttat tcacctgaaa
    21361 ggtttaagtg gctgcaaagt taagtccccg aagccgtaat gaccactaaa ccttgttaac
    21421 gaataccata tttgcggtat tatgctgcta agcgaccaat ctcctacctt cggtcgccct
    21481 gtctttgtaa gtttgcacat ctttagaatt attctaaata gtcaaatttt caaatttctt
    21541 caatgaacga attcataatt gcctttagag agtgcctgga agctgccctg attgttggga
    21601 tcatctatgt ggtactattg aaaaacaatg ccaaatcgca aatcaaaatg gtatggctta
    21661 gcgtggccgc tgctgtcacc gcttctgcca tcatgggtgt agggctttat gaagtgttgg
    21721 aatcggcagg tgatgctttt aaggagtttt tggaaggaat attcatgtat atcacagcgg
    21781 gactgctatt ctatgtgatt ttctggatga gcaagaacct ggcatcgcgg gaggccatca
    21841 ccaaccagac taaagccaca ctttcgcaag gatcgcgatg gggaatcttc tttttggtgt
    21901 tttttgccat tgtacgcgaa ggatttgaaa ctgcactttt tctggttgcc agcaccggaa
    21961 tcgacaagag tttctcctat atcggatttt taaccggagt agcattggct gtgctgattg
    22021 gttatttgat tgtggtgcaa ggaaaacgta ttcagttaaa gccattcttt aaatggacat
    22081 ctttattact cgttgttttc gctgccggta tgatagctta tggtacacat gaaattcatg
    22141 aatacatgga ataccgcgaa catgaagaac atgcaagtgc cgaggctact gaaactgtaa
    22201 acgaagaatg tcacgaatta ttagagcatg ccgtaggcgc agagagtgcc gaaagtgagg
    22261 ctgaggaaga agaggaatat gtattcgaca ttttcaaaaa taaaattgga aatgaaccac
    22321 ttggtggcat gtatatttat gatgaagact gtggtgtgaa tgtgcacatg ctcaatgcca
    22381 atggattaat aggatcattc ctgaaaggat tattcggtta taatacaagc cccacattaa
    22441 tggagattat tttgtggttg ctgagtttag gatttggaat atttatttgg cgaagagctt
    22501 acaagtaata aaaaaaggct gctcataagg cagcctttct tttatcgcaa ttatttttca
    22561 acgctgtact accgtaaaag caaagttggg acccttgcct aatttatatg cagcggtaac
    22621 ccacatttta gctaagttct cgagtaatac ggcattttgc aatccaccca tatccattac
    22681 cctgaaacca agatcttctg ctaatccggc cacacatttt tttgcatcgg catcatcacc
    22741 acaaataaat gtttcaatag cagtgccatt gtaaatcgga ttcagcatat tacctactcc
    22801 ggtagtatta aatgctttca ccacattacc atttcctgtc catgctgcaa ttgcttcagc
    22861 cgccgaatcg aaaggctcgg tagatgcaga gattggattt gttgtatcaa taatgatgac
    22921 attgtcccag tcgaactgaa tattttttat cacctcatcg gtaacaccaa atggaacggc
    22981 aatgagtacc acttcggcac cttgcatggc ttcctgaaat ggtttcactt cggcgccgat
    23041 attgcgcgca tcccatgctt tttgactttc aggatcacgc acaccaaatg tgatggaatg
    23101 ccctttgtct ttccattttt tacctgcagc aagaccgata tttcctgctc ccaaaattgt
    23161 aatatgcata tcagcaaaaa ttaatattag ccttctaaag tggcttccat tgtaatttcg
    23221 gtattcaaca atttgctgat agggcaattt gcttttgcct cggcagcagc tgcagcaaat
    23281 gattcggcag tggcgcccgg aactgttgct ttcactacaa gatgactgtt agtaatagca
    23341 ccgttgtcga gcgtgatggt gcaagtagtc tcgattttat cgggagtaag atttgcagct
    23401 cccaatacaa agctcaactt catgctgaaa catcctgcat gtgcagccgc caccaattct
    23461 tccggattag tgccaatgcc atcagcaaaa cgagttgacc atgaatattg attgttattt
    23521 aatacaccgg attgagtgct cattgttccg gcgccttctt ttcctgagcc gttccaaacg
    23581 gcggttgcgt tccttttcat tagtatgttt tatttattgc aaaggtagta gttgcagggt
    23641 acattgtgcc tatcgacaaa aaaaaagcca cagtttcctg tggccttttc agtatcattt
    23701 tacccgatta acgggcaatc ttcaattcat tgataagatt gcttgccccg gcatatttgt
    23761 cgataacaaa caatacatag cgaatgtcta cgctaatagt tctattcatg cttggatcga
    23821 aaatatagtc tccgggagtg ccttcatagt tgccatcgaa agcaagacct atcagttcgc
    23881 ctttgccgtt catgataggg ctaccgctgt tacctcctgt aatatcgagg ttagagataa
    23941 aacatacagg aacagtacca tttttatcgg catagcgacc aaaatcttta ttcttccaca
    24001 attcatacat tttgtcaggc accacaaatt cttcgttggt attatcgcgt ttttccatga
    24061 taccatcaag cgttgtttgc cagtggtaga atacggcatc gcgtgcaata taatcctgaa
    24121 cggtaccgta tgtcaaacgc aatgtgaagt tggcatcagg ataaaatact ttgcccgcat
    24181 tttgttccag tattcctgcc acatattcgc gttccaaacg gttgatctct gtatttgatg
    24241 cgcggaaagt tgtgcctgct gtttgtgcat cggtatacaa tgcgatataa taattataca
    24301 gcggatcttt tttcaatttc ttcgcgcttg gcgatttcaa aaatgcctca agtttttctt
    24361 ttgttgtgaa aacagatttg gcaaatgcag ccttgctcca attgtcgaat ttttcttgtg
    24421 gagtagcacc tttcgattta gcaaggatat cttttaatac ctgaggatat ttttctgcag
    24481 gtaatttagt gtacaacata ttcaaaatgg ctgcggtctt tttaacctca gtttcatagt
    24541 tgtatgtctt ccacatttct tctgccgaag cttcaattcc tgcaatggta gcagctttca
    24601 cttcttcaga aggtttttcc tcaccaaaca atgtttcaat atcattaaaa tccaaggcaa
    24661 agcttcctga aggcacgatt actaccgaat aaatcttgta gtaaaactct ttgtttacgg
    24721 tgctcagggt gctgtatgta tttttgaaat tttcaaacat ttctttatac atcgcttctt
    24781 ttgcactgcc ttgagttttt gcccatttca tgaaagcttt ttcacgatct tccttgatct
    24841 gcatggcatc catacgttcc ataccgtcga tctgtgtttc ccacaatttc aaagcattca
    24901 tcaatgaagc gtaatcagat gcaagctgga tgcgcgttgc ttcactctta tccatatcct
    24961 gcttcatcac atcagtaacg gttttaaata catcgatctg tgccggatta ctttcagtaa
    25021 ttacttcatt cataccgaaa gaagtaagat aacgctcagt tgatccggga taacccataa
    25081 tcatggtgaa atcgccttct ttgtatccat cgagtgatac tgtgaggaat tttttcggtt
    25141 tgtaaggtac gttttcaggg ctgtattccg cagcattatt ttgcttatcg gcatacacac
    25201 ggaagatgct gaaatcgccg gtgtgacgtg gccacatcca gttgtcagta tcgccaccga
    25261 attttccgat agatgaaggt ggagtaccta caaggcgaac atcattatat gatttgtaga
    25321 gatagatgaa atattgattg ccataataca tggatttcaa ttctgcttcg tatcctttct
    25381 cagcatattt tgcaatcatg cgattttgga tctcagttac tttagcacga cgtgctgcca
    25441 tgtctaatcc ttccacttta ggtatgatgg agtccgtaac atctatcgca tcctgtaata
    25501 tgcgaattgc taatccggga ataggtaatt cttctttgta actctttgcc caaaaaccat
    25561 catccaggta attgtgctcg gttgtagatt gagaagcgat agcatcataa ccacagtggt
    25621 gattagtgaa aagcaaacca ttttcagaaa cgagctctgc agtgcagaat ccgctaccat
    25681 cttgtgtcaa ttgcacaatg gcgtctttca gactggcgtt ttcggtgtta tagatctcct
    25741 ctgctgagag ttgtaatccc atctgacgga ggtagtcgaa gttgagctgg cgaaccaggt
    25801 tggccaacca catcccttca tcgggattca cggcggcgaa tgcaggtctt acaatggagg
    25861 taacacttat taccaatgcc aatgccagta aatgaagctt acgttgtatc atgctgtaat
    25921 gttaaaatga ttctgaccgc gaagttacgg aactgcgccc acgaaagggc agaaggcata
    25981 aaaaaagcgg ccagtagccg cttcgtttta acaaactttt attcttcttt cagaaatttc
    26041 ttcgtgataa gcgtgcttcc ctgtgagtag gcagccaaaa aataccatcc gggctgtaaa
    26101 tcaaccaggc taaccttgat ctcatctttc ccggcttcag gaactatcgt tttgatctcg
    26161 cgaccaacaa tattgagaat gcatatgcga tcaacgtttt catacgtaaa cgagatggta
    26221 gcatagcttt ttaccggatt cgggaaaata ctgatggtcg gatcatcgcc gaaatagccg
    26281 taagctctcg acaatgccgt gctttcctcc attacacaag cttcaccatc tcccgaagga
    26341 agtgtggcaa aggcaccggt gactgtcgca gagaccagta acatgaatgt gtagagtttt
    26401 cgcataggct gtgacgctga aagtaaagat actataactt tttttaaatc tcaaaaatca
    26461 ggccatgtgg atattgataa aattaaccca ttttaaccac ttttgacctt gttcattgcg
    26521 tttttacgga tttcaggtca aaaggttcac ctattggtca tcagggtcgc caaactgctc
    26581 taatgcgtcg aaactgtcgt cgctgaacat gcctgacagc aggtcggtga actgcatgcc
    26641 gctttggagg aaatgcttac tcagcaagga atactcggca agtccgtgga gcaaaaactc
    26701 catcaaaaat agcttttcct cttcagtctt atctccgtgg aagcgctcta caattttctt
    26761 caaaccgggt attttctgca aattggcctt gtgagtgcgc cccgaggtgc cattcaggat
    26821 gtcgagggtg ttgccatcgt taaaccaatt cacaatgtcc tgatacatgc ttccggtacc
    26881 cgggtctgta ttgccggcca acttcttgtt tcccaccttt ttggccttct ccggctccgg
    26941 aaaatagttc gcaaactgcg ccctgatagc ttttccgatg agattcaagg ctacaatgta
    27001 aggaccttcc tgttcaccct catacactaa ttctattttg ccggttatcg acggaaccaa
    27061 tccccaaaaa tcgccaacgc ggacatgtgt agatgactct ccattcacca aagctcggcg
    27121 ctcagcagcg gataccagat tttcgagagc ggttatggtc atcctggcgc ttacgccact
    27181 tttttgatct acgtattcac tttttcttgc ttcgaaggct atttgttcaa caagatcgcg
    27241 tgcgaggttg ttgagcgtaa caactcctgc ctgttctttg tgaatttgtg cctcctgtga
    27301 agtgatgatg cgcgaagtag ctatgtcttt gggataatga gtgagtatct gactttctat
    27361 cctgtctttc aaaggcgtaa caatggctcc gcgatttgta taatcttcag gattggctgt
    27421 aaatacaaat tgaatatcca gaggtaaacg caatttaaat cctctgattt gaatatcgcc
    27481 ttcttgcaga atattaaata atgctacttg aatgcgcgct tgcaaatcag gtaattcatt
    27541 aatcacaaat acacctctat gtgcatgagg aattaatccg aaatgaataa cgcgctcatc
    27601 gttgtaatgc aattttaaat ttgctgcttt aataggatct acatcaccaa tcagatcggc
    27661 gatgcttaca tccggtgtgg cgagtttttc ggtatagcgt tcatcgcgat gcaaccaatc
    27721 gataggcgtg tcatctccca tttcttcaat gcgccggcgt gcaaaaaatg aaatcggatg
    27781 gagtggatca tcatgtaatt cactgcctgc cacaaatgga atgtaggtat cgagcaagga
    27841 tgtcatcaat cgtgcgatgc gcgtttttgc ttgccctctc aatccaagta aaatgatgtt
    27901 gtgtcgcgat aatatggcgc gttccaaatc cggaattacg gtatgttcaa atccatgaat
    27961 tccttcaaag gcgggtttac cacttttcaa tcgcgcaatt aaattgcgac gcatttcttc
    28021 tttcacacta acggcagtat atccactcgc cttgagttcg cctaatgttt ttatctgttg
    28081 ttgcatagtt gctttcaaaa attaatatca acgcacattt ctgcgtctgt ttctttcgta
    28141 gtcttcaaaa atataatcgc caagtccttt caaacttgtg taaaatgcct tgccctgatt
    28201 gatctcggta aactcctcca caaactgctg caaataactg tcgcgcgcaa tcataaatgt
    28261 ggtaatgcgg atacctaatt ttctgcaatg tgctgccaga ttcagtgttt tatttacaat
    28321 gatgcgatct agcccgaagc tgtttttata atatttccca ttccgcataa tgcaggtagg
    28381 tttgccatcg gtgatcatga atatttgttt gttggcattt tttcttcgcc tcaaaatatc
    28441 catcgccaat tctaatcctg ccactgtatt tgtatgatac gggccaacct gcaagtaggg
    28501 aatatcttta atttctatct gccacgcgtc attgccgaaa acgatgacat caagtgtatc
    28561 ctttggatat tttgtgttga tgagttcgct caatgccatg gctacttttt ttgccggagt
    28621 gatcctgtct tcgccataca aaatcatact atgcgatata tcgatcatca gcacagttga
    28681 tgtttgcgct cgaaattctc gttcacgcac aacaaggtct gactcatcca tactgaaacc
    28741 tgcaatgcca tgattgatat aggcattttt cagcgattca ttgtaatcaa tctgttcgtg
    28801 tgtatctcca aaaacaaagg gcctgctatc ggtagtattt tcgtcaccac tccctgtttg
    28861 agaagttttg tgatttccgc gttgcgattt ttttaattta ccgaaaatct gatccagcga
    28921 tgaacgacgt atcgactgct ccatcttgcc ggtaatgcgc atggtgcctg cctgctgttc
    28981 gtcgtcttgt aaatatccgc ggtcctttaa ttcctgaata aaatcaccca tgccatattc
    29041 gggcgtagtg atttgatatt gcctgtcgag ctgatttaac caatccaatg cttcagttac
    29101 atcgcctgaa gtatatacca ataactcctg caataattca aacagtgtat cgaaggtagt
    29161 ttgtggtttt tcttcgtatt ttgaaaagcg attgcctagc ataataaatg gatatcagaa
    29221 aaatctttta tgaatgtcta attcacctaa ttttccaccg aataaaatat ccctcacgcc
    29281 atcacctttt aaaaaatcgt gcgggaatcc taattcaatg gcgcttactt gttgaagacg
    29341 ctgcaggtgt tcctgtgaaa gtactatttc ggttgcaccg atgctgtctt ttaattgatc
    29401 cgcatttcgt gcgccaacaa taggaataat ttgttgatgt tgctgcatca cccatcgcaa
    29461 tgcaatttgt gcagcactat gtccggtttc atgtgcaata tttacaactt cctgtgcgat
    29521 agctctgctg cgttcattta aacgagcact gttaggctgc aatcttttag ggtcattatt
    29581 ttcctgtaaa tattttccgg tgagtgcacc acctgccatc ggtgcccatg ctgtaacggc
    29641 aagttccata gattgagcca ttggtaaaag gtcgcgttct actgttcttt ggatgagcga
    29701 atattcgacc tgcagggcga taaacggatc ccatcctctc agctcagcaa tggtgttggc
    29761 acgcgaaaca atccaagcag gcgtatctga aatggcaaca taaaatattt tcccttgttg
    29821 aacgagatca tgcagcgccc gcatcacttc ttccgcagca gtagtgaaat cccaggcatg
    29881 cagatacagt atgtcgatat ggtccatttg caaacgcttg aggctacctt ccactgattg
    29941 caccatattt tttctgtgat tacctgccag gttgagaccg cgttcatggg taacaagtgc
    30001 atatttagta gccaccacca aatcgtcgcg cctcccgctc tgtttcacta attcgccaag
    30061 aaagcgttca ctggatcctt cggtgtatct gtttgcggtg tcgataaaat ttcctccggc
    30121 ctccagaaag gcatgaaatt ggcggctgct ctcctcaaat cccgctcccc agccccattc
    30181 ttcaccaaaa gtcatggtgc ccaggcagag ttcagaaacg cgaagaccgc ttctacctaa
    30241 aagtttatat ctcatcatct gcgtaaaatg taattgcaag tatgcccaat cccgctgaat
    30301 gttccaaatc gccaaacaga atcgttgcta acatgtcatg cagttaaaaa ccccaccttt
    30361 gtgaataggt tcattatttt agcagataaa ttaacttttg gatgagacta cgattattgg
    30421 cagctttggc gtgtatcact tttacggcat tccaacaaac cgcaaacgcc caaacggctc
    30481 ctgccaaaac cctcgacgat aaagcagctt tcaatgccat tttcgatgaa gtaatgaata
    30541 atggtgaggc gtatgataac ttagaagtat tatgtaaaga cattggtcac cgactcgcag
    30601 gatcgccgca agcggatatg gcagtgcgtt ggagttatga tatgatcaaa aaatacggat
    30661 ttgattctgt atggttgcag ccggtgactg ttccacattg ggtgcgtggt gagcaggaat
    30721 atggcgagat tatgaatgtt ggaaaagtga atatgcttgc tttgggaggt agtgttgcaa
    30781 cgcctatcga aggaattacc gataatgtaa ttgaagtgcg cgatttcaaa catcttgaag
    30841 aattgggcac ggctggcatc aaaggaaaga ttgtattttt taattaccca tttcctcaaa
    30901 atgtggtgaa ctcatttgaa ggttatggcg acgcgggacc ttatcgctgg catggtgcga
    30961 atgatgctag taaatatggt gctattgccg ttgtgatcag aagcgttggt tcttctcatg
    31021 acgattttgc ccatacagga agtactcatt ttgatgacgg cgtaaaaaaa attccttgtg
    31081 cagcactcag caatatcgat gcagatatgc tgcataatgc cttgcaaaaa gatccttcca
    31141 caaaatttaa aatgatcatg agttgcgcca tgcttgattc ggtgcaatca tacaatgtta
    31201 ttgcagaaat aaaagggtct acttttccaa aggaagtgat tgttgtaggc gggcatttag
    31261 acagctggga tgttggtgaa ggtgcgcacg acgatggtgc cggttgcatg cagagtgttg
    31321 aagtacttcg cgcaatacaa gcggcaggac taaaacctaa acgcactatt cgatgcatac
    31381 tttttatgaa tgaagagaat ggtaatatgg gaggtaaaac ttatggaaaa tatgcctctg
    31441 ccaccaagga tgaaaaacat atcgccgcgc tggaaagtga tgcaggcgga ttttctccac
    31501 gcggatttag tttggactca gcgatgcttg cacgccctga gatggagcaa ttaaaagaac
    31561 tcatgcttaa atatggagtt tatagttttg aggaaggtca tggtggaaca gatattggtc
    31621 ctatggaaaa agcagggaca catttaatcg gattggtacc tgattcacag cgatacatgg
    31681 atttacatca taccaataat gatgtatttg aacaagtaaa taaacgagaa ttacatatgg
    31741 gcgcttcagt gatggccatg atgtgctggt ggataagcga acatggagta aaataatttt
    31801 taagatgcgt agcctattca taaaattcca actgttctgt ttgttgcaat tcattctgat
    31861 tgcaggaaac gcgcagtcta ttacgggaa
//
LOCUS       DAOQOU010000018        30017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_687_length_30017_cov_6.398538, whole genome shotgun sequence.
ACCESSION   DAOQOU010000018 DAOQOU010000000
VERSION     DAOQOU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 30017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30017
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_687_length_30017_cov_6.398538"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1522)
                     /locus_tag="PLM90_02920"
     CDS             complement(11..1522)
                     /locus_tag="PLM90_02920"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMZ88349.1"
                     /translation="MNLNLLILIPVATAIGIIAVKGLPRVRMVALAGALVQFILSFVL
                     FFSFRAERASGNQDPFVFTQYYKWFPSLNINYQIGVDGISIAMIMLTAFVVLAGVLIS
                     WRQEKWSKEYFLLLILLSTGAYGFFIALDLFTLFFFLEVAVIPKFLLIGIWGSGKKEY
                     AAMKLALMLMGGSALVFVGLAGTYFFGGQSFDLSSGAHITMDPDKQMFFFPFLFVGFG
                     IFTALFPFHTWVPDGHSSAPTAASMFLAGISMKLGGYGCLRAATWLMPEAAHYYQPLI
                     IGLSTIAIIYGAFATLMQTDLKYINAYSSVSHCGFVLLGIGMLNETAINGAVMQMVSH
                     GLMTALFFAAIGMVYDRTHTRMVKDIGGLLKITPFIGTVFILAGLCSLGLPGMSGFVA
                     EMTVFMGGWQNPETLYRVATILACASIVVTAVYILRAAGKTMMGPLVNQEHALFSDAK
                     WNERTAAFVLLTGILIMGLAPFIITDIIYPGTHVLMDKIASGASITNEIELLK"
     gene            complement(1595..3514)
                     /gene="nuoL"
                     /locus_tag="PLM90_02925"
     CDS             complement(1595..3514)
                     /gene="nuoL"
                     /locus_tag="PLM90_02925"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HMZ88350.1"
                     /translation="MSNYDYISLIPILPIAGFVLIGLAGKKYFPALSGWLATLLIGTS
                     AVLSLFTAYQYFIVDGAANGVYPQLLPVDMQWLQFTNTLSINMGILLDPISVMMLVVV
                     TFVSLMVHIYSTGYMKGESRYATFFAYLQLFTFSMLGLVMSSNLFQIYIFWELVGVSS
                     FLLIGFYFDKPSAVAASKKAFIVTRFADLFFLIGILITGVTAHSFNFTDIIANLTSPE
                     FLKGYSATFLGFSVVTWALVLIFIGGAGKSAMFPLHIWLPDAMEGPTPVSALIHAATM
                     VVAGVYLVARLFPVFAITGSGAIDVVAYIGIFSALFAALIACTQTDIKRVLAYSTMSQ
                     IGYMMFALGVSSQAHDAGFTASMFHLFTHAFFKSLLFLGAGLVIHAVHSNDMKDMGGL
                     RKYLPITHITFLIGCLAIAGVPPFAGFFSKEGILHAAYTTHPDIYYVALVTSGLTAFY
                     MFRLYFRIFWNKADIHLHHFHGEGAWSMKFPLLILATLSIAAGFVPFAHYVTATRTEG
                     HMHTDIMFSIAPVLIGLLGIAVAMMWYKNENDRADKAVAMMRGVYRTAYHKFYIDELY
                     IFITKKVIFNCIGRPAAWIDRNIVDGTMNFIALMTGEISDSIKGMQSGKVQGYAMYFF
                     AGVIGLTLICLYIWM"
     gene            complement(3517..3831)
                     /gene="nuoK"
                     /locus_tag="PLM90_02930"
     CDS             complement(3517..3831)
                     /gene="nuoK"
                     /locus_tag="PLM90_02930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HMZ88351.1"
                     /translation="MNNIGIEQILFISTALFFIGVYGLFTRRNMITMLMAVELILNSV
                     NINFVSFNKYLYPERLDGVFFTIFIIAIAAAEAAVAIAIIINIYRNHQSIDVEDSEEM
                     KY"
     gene            complement(3828..4346)
                     /locus_tag="PLM90_02935"
     CDS             complement(3828..4346)
                     /locus_tag="PLM90_02935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMZ88352.1"
                     /translation="MNAYSIIFYLLAAFIIGNGVLAMTTRKIFRAAIFLLFSLIGVAG
                     LYFWMHVEFVAAVQVIVYVGGIVVLIIFSIFLTQQTGKDMPRPTNTRMIFSALAAILG
                     AALTYSLIRQYGFIGGSGDQFNVSPQRIGDQMLDYQDTGYALPFEVISILLLAAMIGC
                     IVIALKSKPQES"
     gene            complement(4399..4869)
                     /locus_tag="PLM90_02940"
     CDS             complement(4399..4869)
                     /locus_tag="PLM90_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ88353.1"
                     /translation="MFLSKYISEVFGGVKSLLTGMRRTGYYFTHHKNIITQQYPNQKP
                     VLAERFRGEVVMTHDENNEHACTGCTACELACPNGTIKIVTKFDITPEGKKKKAIDTF
                     VYHLELCTMCNLCVDACPTGAITMSNSFEHSVFDRSELTKVLNKPGSKIREGVE"
     gene            complement(4988..6067)
                     /gene="nuoH"
                     /locus_tag="PLM90_02945"
     CDS             complement(4988..6067)
                     /gene="nuoH"
                     /locus_tag="PLM90_02945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMZ88354.1"
                     /translation="MEFSLASIADSIDQWLNAHCASWLALTIEFVIVGVLCIGLFAVL
                     GLVLIMMERKVSAYMQLRLGPNRVGPKGMFQTVADTLKLLVKEGLTPAGADKFLFNLA
                     PFVVMIASMLVLAPIAFAKDFVIWDVNIGVLYVSAVSSIGVIGILMAGWASNNKYSLL
                     GAMRSGAQIVSYELSAGLSILTIVIFSQDMSISSIINSQQDGWWIFKGHIPVIISFVI
                     FIIAVTAETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYVNIFIVCAIGATLY
                     LGGWMPLHIGNWEAFNHIMDYIPSSVWFLGKTFFLIFVIMWFRWTFPRLRIDQLLNLE
                     WKYLLPISMFNLLLATLVAIQGWYF"
     gene            complement(6170..7285)
                     /locus_tag="PLM90_02950"
     CDS             complement(6170..7285)
                     /locus_tag="PLM90_02950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMZ88355.1"
                     /translation="MLQEVAKTDTGDLVINVGPQHPSTHGVLHLVITLNGETIKKVEP
                     HLGYIHRSIEKMCESLSYRQFIYSTSRMDYLSAHINNHGCALAVEKGLQIEVPQRAQV
                     IRVLMDELTRLASHQLWWGALAMDVGAITPFFHAFRERELINDIMEETCGARLTMNYM
                     VPGGVMFDLHPDFQKKVKDFLTLFKSKLPEYDELVTGNIIFQNRMKNVGYISKEDAIS
                     YGCSGPTARASGVSCDIRKKYPYDIYAQLQFSEAIETAGDCWGRYLVRMEEMRQSISI
                     IEQLIDNIPEGDFVAKTKAVLKLPKGEFFTRVETARGELGIYIVSEGGLTPYRIKFRS
                     PGFSNLSALDHMAKGQKIGDLVAIMGTLDLVIPDIDR"
     gene            complement(7354..7809)
                     /locus_tag="PLM90_02955"
     CDS             complement(7354..7809)
                     /locus_tag="PLM90_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMZ88356.1"
                     /translation="MTREELQAAITNLEPQVTFEEGGEFLNAVVPSELCRDLMQALHN
                     MPDLSMDYMFCLTCVDWKTHLTMVYHLESTAHGHQVVIKSQLNRENPEIETLSDIWRT
                     AELLEREVFDLFGVRFIHHPDLRRLLLTDEWEGWPMRKDYEDPINMIKL"
     gene            complement(7828..8382)
                     /gene="nuoB"
                     /locus_tag="PLM90_02960"
     CDS             complement(7828..8382)
                     /gene="nuoB"
                     /locus_tag="PLM90_02960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HMZ88357.1"
                     /translation="MDVKEPGYQQQPFPGDVHPTATGGLLVSRLDDVINWARSNSLWP
                     LVFGTSCCAIEMMSTASAKYDWSRFGFEVARATPRQADVIIIAGTIVNKMAPVLKRLY
                     DQMADPKYVIAMGACATSGGPFFYNTYSVVKGADHVIPVDVYVAGCPPRPEALLHALI
                     TLQTKIQAGGKREKIVIEKFYDNQ"
     gene            complement(8373..8723)
                     /locus_tag="PLM90_02965"
     CDS             complement(8373..8723)
                     /locus_tag="PLM90_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HMZ88358.1"
                     /translation="MGPASLLVLIMAAVAFSTAGILLSKVVLKASKNPVKGEPYECGV
                     PTIGQNWVQFNVGYYLFALIFLIFDVELIFLYPWAVSVQRLGMFAFTEAVIFLFILFM
                     GFLYAHKKGALKWM"
     gene            9260..12835
                     /gene="nifJ"
                     /locus_tag="PLM90_02970"
     CDS             9260..12835
                     /gene="nifJ"
                     /locus_tag="PLM90_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HMZ88359.1"
                     /translation="MSHTESQLISPDVKHGNFNFKRDIMDGNEAAATIAFKTNEVCAI
                     YPITPSSPMGEWADEWSSKGVINIFGQVPTVIEMQSEAGAAGAIHGALAAGALSTTFT
                     CSQGLLLMIPNMFKIAGELTPAVFHIAARAVATHALSIFGDHSDVMAARATGFSMLFG
                     CNSQEVMDMALISQSATLSARVPFMNIFDGFRTSHELSEVEIIPDEIIRAMIKMEDVM
                     AHRSRAMNPANPFIKGTAQNPDVFFQGREASNKYYMQVPEVVKAKMKAFASLTGRTYN
                     LFDYYGHPEAESIIIIMGSGAGPVQEIVDYLNANGGKTGVVIVRLYRPFAIDDFVHAI
                     PSTVTKIAVLDRCKEPGAIGEPLYMDVINALREKERDIRKVIGGRYGLGSKEFNASMA
                     KAVFDELNQPKPKNHFTIGIDDDVTNTSLHFDPDFNLEKQYYFRGLFYGLGADGTVSA
                     NKNSIKIIGDHTGDFVQGYFVYDSKKSGSLTVSHLRAGKNPIRSSYLVQSANFVACHH
                     FNYLLKYDVLRYAEHGAVFLLNTPYPHEEVWQHLPKKIQEEIIEKQLKFFVINASAVA
                     RQAELGTRINTILQTCFFAISNVMSSDEAIADIKQAIHDSYIKKGISVVEKNYAAVDK
                     ALANLFEVDYTKNATGNLEGDKIVPDDAPDFVQKVLARIMAGEGDELPVSAFPVDGRY
                     PAGTTQYEKRNIAEQVPVWDADLCSQCGKCFMICPHAAIRPKVYAKEALADAPPTFRH
                     VAPIGKEFNKEEEAYTLQVSVEDCTGCNLCVAYCPVESKLEKGHKAINMVDKLGLEES
                     EQANWDFFLSLPDIDREKVNKNTVKGTQFLQPLFEFSGACAGCGETPYLKLLTQLFGD
                     RMVVANATGCSSIFGGNLPTTPWTTNHEGQGPAWANSLFEDNAEFGLGIKLATDKKRE
                     IALYLLDQMQPMIDAGLYDAIKSHHGTAESDINIYKQQIAALRKQIRGIDNPNAHMLL
                     HFADYLIEKSIWIIGGDGWAYDIGFSGLDHVLSTGENVNIFVLDTEVYSNTGGQKSKA
                     SPLGASAKFSINGKRSGKKDLALQAIAHGKAYVAQIAMAANDMHTVRTIQEAEAFPGP
                     SLIIAYSHCIAHGYDMCHGSDQQKNAVNSGYWPLFRYNPLKARGERFTMDSKDPSIPL
                     SEFLYHENRFASIRAKDPALAETFLHMAEADLKAKYERLHLLESM"
     gene            13174..14280
                     /locus_tag="PLM90_02975"
     CDS             13174..14280
                     /locus_tag="PLM90_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMZ88360.1"
                     /translation="MSTLKKTFFALTLLFATQQFVQAQTTEQAFSPSIKVKALIHSRF
                     EASLTDSIDVAGKYFANPVTTNFRMRRIELRTDIKLNDKFSGVVRVQLPELKGSSVGK
                     VIELAYMDYKYNDMLNVRFGQFKSPFELDEQTSHEDLRMIDRGPTSVMFVNNSLASYQ
                     PGAMVFGTFMKSKTPLSYYLAFVNGSNRAVNNDDNSQKNIVGRLEFVPIKGVKIGANA
                     QQIYTKDTSGLSYGGDLSIIRDLNDKMKLIVEGEYITGYQVNDFNADTTGLEIGDYTM
                     GGYFGQVLLRSELNKSWCKTFEVGGKYEHTDPNTTVDDNAFNTITGGIGFIFTPDNMA
                     RLQLNIVHTDYEKTITPGGLMAANMFVTQFQLKF"
     gene            14399..16312
                     /locus_tag="PLM90_02980"
     CDS             14399..16312
                     /locus_tag="PLM90_02980"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ88361.1"
                     /translation="MNLKYYIVLLIANSLQLYAFAQSMPDQMVISDDGTRLSVGGQEP
                     NDFYNIDSIRSINLIFDEPNYWSALTANYASKTDLAATMVYNGDTLAEQVGVRFKGQT
                     SYMMNMTDKKSFNITIDYIDSSQDIHGYNTLNLNCGYEDPSFMRESVYENNIRKYIPG
                     LAVNYVHLYLNGEDWGLYLNVQQLNKDYYKEWFMTNDGTSWRAEKEGFGGPGGGDPFG
                     AGYCSLNYLGTDSSDYTPYYQLKSTDLEDPWTDLINTTDVLNNTADASLEDAVNNVLD
                     LDRTLWFLALEIIFSDDDSYVNKGGMDYYVYWEKETDRIVPMEYDGNSCMDPMHLSWT
                     PFYHETDADYALLNVLLGNDNIRQRYLAHMRTILDELFNSDYMDAYIDAMDTRIDALV
                     NADPKKIYTYAQYNADVTELKTFVEEHYNDFINNTEIDRLDPEISETLMYSAAGDWIA
                     PLSMEAPVVTTNVYTESNINGVNLYYGTGLVGRFYPLQMFDDGAHNDGLFNDGTWGAN
                     LPAMPAGTDVRYYIEAIADDGYGTRSYDPVGAEHDVYYYQVRGNVAVEDFIAETINVY
                     PNPATDMVQIILPSSNETADIELFSLGGNSVLNQLNYKSGAPLNISKIAPGCYFLHTK
                     QNEVTAITKIIIQ"
     gene            complement(16366..17223)
                     /locus_tag="PLM90_02985"
     CDS             complement(16366..17223)
                     /locus_tag="PLM90_02985"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ88362.1"
                     /translation="MMNRTRINVLMLSVALLPFAFSSCDEVGPTIDLTDTEYETGVGT
                     PETPQNKVVLIEDFTGAACPNCPDAHIAISDAISAHPGRVTAIAEYNYFADPLYIEQN
                     LTTTESYQLDDYMGPVVGWPASFIDRTDFEADGYIPEVPANIASYVNDQLALSSPCNI
                     EITPTFDAATRNLHVLVKVAYTSDVTNVNHLSVALVESGIIAAQIDDNVGGEVEDYVH
                     NHVLRKFLSFYLGEALPEENVAGRWYTFEYDYTLPDTWNADNMAVIGFVHNFETENKT
                     VIQAAEADL"
     gene            complement(17234..18823)
                     /locus_tag="PLM90_02990"
     CDS             complement(17234..18823)
                     /locus_tag="PLM90_02990"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HMZ88363.1"
                     /translation="MRKSFLVALMIASAMTVFGQGAISGDLQLNTEFYQRDSLIGATG
                     TPHYDNLLSGANSWLTTYYRNDAWGLEAGVRLDMFLNSNLHNPGIPYTDAGIGRWYIS
                     KKIDAFKITGGYIYDQFGTGLTFRAYEERPLGIDNALFGVELEYQINDDWKIKAIGGQ
                     MKDLFTRYEPVIKGATIEGLLPTKNSNITIAPGASFVNRTIDQDNMDIIVAQINSYDL
                     ADRFVPKYNMYAGSIFNTLSIKSVTWYAEYAMKSTDAIRTPSGILDDSKGNVLYSSLT
                     YSTSKIGDGFGITGQVRMVDNWVMRVSPNTTLLDGIMDYLPSLTKQNSLRLTARYQAV
                     AQELGEFAYQVNGTFTPKKGYTININYSNVTNDTTELFTEVFADFEMRKQKYKLLLGG
                     QLVHYNQAIYENEPGRPMVEAITPFAEFTYKFSKKKSMRLELQYQHCEQDFGSWVYGL
                     VEYNIAPKWSFAVSDMWNYDPLKTDDALHYYSVFAGYTQKSTRFTLNYVKQIAGIVCT
                     GGVCRYEPAFSGLKAGITTTF"
     gene            19053..19547
                     /locus_tag="PLM90_02995"
     CDS             19053..19547
                     /locus_tag="PLM90_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ88364.1"
                     /translation="MKKFVLLSATLFAAITMVNAQDKLPSLELSDVNGNKINVADYSN
                     DSTIQVFSFWATWCVPCKEELNNIAEIYEDWQKDYKVEIIAVSIDDAKTKANAKSYAE
                     GQGWTYTVLLDTNKDLQRLLGGQSVPFTVITDKSGNIVDKHTGYIEGDEYELEDKIAK
                     LAKE"
     gene            complement(19578..20726)
                     /gene="hemW"
                     /locus_tag="PLM90_03000"
     CDS             complement(19578..20726)
                     /gene="hemW"
                     /locus_tag="PLM90_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMZ88365.1"
                     /translation="MYINDQEAGIYLHIPFCRKACHYCNFHFSTSLQSKEAYLLAMHS
                     EIDARVGYTEGKIIRSIYFGGGTPSVLTADEISSLLAHIKQLHTVRADAEVTLEANPD
                     DLSKPYIESIHAAGINRLSIGVQSFIDHDLQWMNRTHTGSQAKQVITDAMLSGFEHMS
                     ADLIYGIPGSSHEEWRSNIQTLIALKVPHISSYALTVEPKTALAHFIQTGKSEMPDEQ
                     LAAEQFEILRHMLIDAGYDHYEISNFALPGHTAIHNSAYWAGYYYLGIGAAAHSYNGV
                     SRQWNINNNALYIRYDKSGNEYYTIENLDMHQRMNERIMTALRTTRGLDLERFLSEFG
                     EVYHNQLTNEIRRLESAEKMIIENNIARISPSHLFIADSVISDLFFVD"
     gene            20797..22557
                     /locus_tag="PLM90_03005"
     CDS             20797..22557
                     /locus_tag="PLM90_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ88366.1"
                     /translation="MNFFLWHHAHAQDSIYIVTNYNNQLNEYLSDFNTTFATIQEAEH
                     ALNDLVHTMQIDGYLTAAWRWEPNEMHRNPSVKVVEINLGKKVLWANLDASGVEPWLL
                     RAAGFDPEDWINTTYNQNKAASFLQELVSYANNNGYPFASSHLDSITYIDSTLSAKVI
                     FEKNILIFFDTIKVIGSLQVTSNFITQYTQIISGQPYDQSLIDALPSRLKELNFLQQT
                     KAPQIEFMGNKATVLIFADDKRSGSFDFLLGVLPNNEITGRLIITGDMRLHLQNVFHS
                     GEEIHFQYTKLESASKSMDAAFTYPYLPHIPLGPDASFHLYLKDSTFLERKASAGVVW
                     QIAGNHSLKGFTSFYNSSVLRPDTTFVLLNYALPSMLDVKENAYGLTWQYQHLDYTFN
                     PRKGYAIALTGTAGTRTILENVSITELFNPLTPDFDFSTLYDSIENKSLSLKYQYDLQ
                     YFIPLYNRMAILLQVRGASILHDNIVENELYRIGGNQLLRGFDEQSLPVSDYHIGTFE
                     YRYLLSQNAYTSVFVDAGVTKNNALDNTALVYPYGFGAGVRFETKAGIFGLSYALGGS
                     NMNPVQVKNTKVHFGYINYF"
     gene            22557..23447
                     /locus_tag="PLM90_03010"
     CDS             22557..23447
                     /locus_tag="PLM90_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HMZ88367.1"
                     /translation="MKRITVAACLMVSLSVTAGAQETILQNPAPDTLIHYAYDTAALM
                     KRMHFAPVDYDSLLASPNTNRKTAPFSSFHVEKRDLLLPGLCLLMLIYVTWLRYTYGK
                     ELRENFTVILNTNLGQQIYRDREFSANIFKFLTFINFALGGGMLVYLTTTYYHVNLPF
                     NQTGLNVALMVMAVFALYLVKGFVYRFVGAVFNITASLRFFRFNALVIYHLLGIGLLP
                     FILLAAFAEPPVNTWALFAALILVAIAIVLRVFKGLTVALSSGKLHILYFLLYICALE
                     VAPLLIAIRLFDLWAGSQPK"
     gene            23455..24237
                     /locus_tag="PLM90_03015"
     CDS             23455..24237
                     /locus_tag="PLM90_03015"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HMZ88368.1"
                     /translation="MNKPQPTSLKPVKSILVSQPKPPEVPKSPYQEIAEKYKIKVEFR
                     QFIQVRGLSSKDFRKQRINILDYTAVIFTSRNAIDHFFRICEELRIRMPEMTKYFCVT
                     EAIAVYLQKYIQYRKRKVFFGTGKDTDMFDLFKKHISEKFLFPCTVNHKDIYTEYLKS
                     INADFSEAFMYETVPADLSDLKGKIDYDMIILYTPTGVASLFQNFPGFEQGDLRIGCM
                     GASTLKAMEEQNLRVDLKAPSHDSPSITMALDKYLAISNKKK"
     gene            24484..26538
                     /locus_tag="PLM90_03020"
     CDS             24484..26538
                     /locus_tag="PLM90_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ88369.1"
                     /translation="MPTNKLINETSPYLLQHAHNPVDWYPWGEEALEKARTEDKPILV
                     SIGYAACHWCHVMEHESFEDDTTAELMNKYFINIKVDREERPDLDNIYMNACQILTGA
                     GGWPLNVFLTPELKPFNAGTYFPPKPGYGKPSWKELLMYMYQVYADDRDKVEHQANLI
                     MEHIAKMDGAFLEKNFVLESENIFSKQQLDAAINTIQSNFDRVDGGFGSAPKFPATMT
                     LRFLLRYAWFTNNKPILDHVILSLNKMHQGGIFDQIGGGFARYATDNKWMIPHFEKML
                     YDNALLCSLYAEAYRATGDNNYLQVVKDIIQYIDRECSDELGGLYAAYDADSEGVEGL
                     YYTFTYQDLENALGDDAVWAAKVFNATSAGNWEHTNILHRDASDETTARQLDMTHEAF
                     SAKLKLVKGKLLQYREKRIKPALDNKILLSWNALMCSAFVDAYKASGEEQFRQKAIQL
                     VQHLTSAFKRNNTENAYWHVITNNIAKQTAFLDDYATLIQALFDVFEITGDEAWFKQA
                     DGCMEYVIANFSATDGMFFFTEKSQTDLPSRNAEYYDNATPSGNSIMLSNFQRRSIML
                     GNTKDVERVRTMLGAMKNSLIKYPTSFGNWWLSALKQIYPSVEIAVTGPDHQVMRDRI
                     FTHYHPHILVQNGNNAVSEFPLLQHRASDVETRIFVCHNFTCKKPVTTLQDFVSEVQT
                     LG"
     gene            26603..27601
                     /locus_tag="PLM90_03025"
     CDS             26603..27601
                     /locus_tag="PLM90_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMZ88370.1"
                     /translation="MKQIYARITCGLVFSVAVFNAQAQLISYGQQDPQFTQYMLNTSG
                     FNPAAIGVEDAWVSTLGVRSQWVSLPGAPVSQSLTSHAPIYKINSAVGVTVINDIMGQ
                     QRTTGASLDYAYHKRFGPSTLSIGASGGIFQETINGAKLVTPTGNYEGGVNHNDPNLP
                     VTTISKMIPDAAFGMYFFGRNLTAGISANHLLLPITAGNDANSGTVQYNPNGYVYVAY
                     GFDAGNRFRIMPNVLYKTDLNEGVVDLNTLVTYNNNIQAGISCRANTALAFDAVALMA
                     GMRVADRWLLSYSYDVTLSALNTVSSGSHEIVVRYSIPVARPRAGKMINNPRFLYH"
     gene            27749..29032
                     /locus_tag="PLM90_03030"
     CDS             27749..29032
                     /locus_tag="PLM90_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ88371.1"
                     /translation="MKRNLQLFTLAFVAIIVGSGCSHSSWDDYRGQLLGMQDRPEWEP
                     VNPYGMVYIPSGTLHIGPSDQDVNNSLWARAKSISIQGFYMDDAEITNNEYRQFVYWV
                     RDSIMHTLLDHYVEEEEGMESEVPLIDWSQEIDWDDEETRSRLEELYLPASETYYGIK
                     AIDTRQLQYNYRYVKWKEAASKSNRDKNIAEFIVEEPIEIYPDTLVWVHDYTYSYNEP
                     MTRQYFSHPAFDDYPVVGVSWKQAGAFATWRSDFWNDWRRAHEEVQVDDFRLPTEHEW
                     EYAARGGRDMAPYPWGGPYVRNTKGCYLANFKPGRGNYPEDGGYYTVKVYSYAPNDFG
                     LYNMAGNVSEWTSSAFQENTYSFLHDENPDYRYDAEDSDPPALKRKVLRGGSWKDISY
                     YIQTGSRHWEFQDTAKCYIGFRCCMTFLGRSMSDF"
     gene            29117..29941
                     /gene="gldL"
                     /locus_tag="PLM90_03035"
     CDS             29117..29941
                     /gene="gldL"
                     /locus_tag="PLM90_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HMZ88372.1"
                     /translation="MSAFFESDRFKRIKNLIIGIGASVVLVGALFKILHWPGADQMLM
                     VGMLTEAFLFLMLGIIPPHKDYYWEKIYPDLDIAPEVEEAKSGKKVHKMTEPKESVTR
                     QLDKMLEDSKVEPVMIERLGENLKKLGENIEKLSDVQDAAVNTNEYSNKIKDASTAVA
                     EMRDAYANAARAMEQITASSVDTSKYHEQVQMVTKNLSQLNAIYELELQDTNNHLKAM
                     NKFYGSLNSAMDNLQESLTDTSKYREEIAQLSNNLAALNRVYGNMLSAMSMGAARS"
BASE COUNT         9088 a         6515 c         5995 g         8419 t
ORIGIN      
        1 atgaatgata ttattttagt aattcaattt cgtttgtaat agatgcacct gatgcaattt
       61 tatccatcaa tacatgagtg cccggataaa tgatatcggt gataataaat ggtgccagtc
      121 ccatgatcag gatgcctgtg agcaacacga atgctgcagt gcgttcgttc catttagcat
      181 ctgaaaataa ggcgtgctcc tgattcacaa gaggtcccat cattgttttt cctgctgcgc
      241 gaaggatata aaccgctgta accacaatac tggcacaagc taaaatggtg gctacacgat
      301 ataaggtttc cggattttgc catccaccca taaatacagt catttcggca acaaatccac
      361 tcatgcctgg caagcctagt gaacataaac cggccaaaat aaatacggtc ccgataaatg
      421 gcgtgatctt caataaacca ccaatgtctt tcaccatcct tgtatgcgta cgatcgtaaa
      481 ccatgccgat agcggcgaag aaaagtgccg tcatcaaacc atgagatacc atttgcatca
      541 ctgcgccgtt aatggctgtt tcattcagca taccgattcc caataacaca aatccgcaat
      601 gcgatacgga tgaataggca ttgatatatt ttaaatcggt ttgcatcagc gtggcaaatg
      661 caccgtaaat aatggcgatg gtagataatc cgataataag gggttggtaa taatgtgctg
      721 cttccggcat caaccaagtg gcagcgcgta gacaaccata tccgccaagt ttcatgctga
      781 taccggcgag gaacattgat gctgctgttg gggcactgct atgtccatcc ggcacccagg
      841 tgtgaaatgg aaacagtgct gtgaaaatgc cgaagccgac aaataaaaac ggaaagaaaa
      901 acatttgttt gtccggatcc atggttatat gtgctccgga gctgaggtcg aaactttgtc
      961 cgccaaagaa ataagttcct gccaatccca caaataccaa tgcgctacca cccattaaca
     1021 tcaaagcaag tttcatggct gcatattctt tcttcccgct gccccaaatg cctatcaaca
     1081 aaaattttgg gataacagct acttccagga agaaaaacaa ggtaaataga tcgagggcaa
     1141 tgaaaaaacc atatgctccg gtgctaagca gtatcagtaa aagaaaatac tctttagacc
     1201 atttttcctg acgccagctg atcaacacac ctgccagcac aacaaaagct gtaagcatga
     1261 tcatggcaat ggaaatacca tcaacaccaa tttgataatt gatatttaat gaagggaacc
     1321 acttataata ttgtgtgaaa acaaaaggat cctgattgcc tgaagcacgc tcggcacgga
     1381 atgagaaaaa taatacgaag ctcaatatga attgcaccaa cgctcctgcc aatgccacca
     1441 tgcgaacacg aggcaagcct ttgaccgcga ttataccaat ggctgtggca accggtatta
     1501 atatcaataa attcagattc atacgtttat ttcacttaat aataaaatat cgcgaatgta
     1561 tttttctctc accactaaat actatccgga taacttacat ccatatatac aggcatatca
     1621 gggtaagtcc aataacacct gcgaaaaaat acatggcata accctgtact tttcctgatt
     1681 gcataccttt aatactatcg gaaatctcac ccgtcatcaa cgctatgaaa ttcatggtgc
     1741 catccacgat atttctgtcg atccatgctg cggggcgacc gatacaatta aagattactt
     1801 tcttagtgat aaaaatatat agttcgtcga tataaaattt atgataagct gtgcgataaa
     1861 cgccacgcat cattgcaact gctttgtctg ctctgtcgtt ttcgttttta taccacatca
     1921 tcgccactgc tattcccaat aatccaataa gaaccggtgc aatgctgaac atgatatcag
     1981 tatgcatatg accttcagtc cgcgttgcag ttacatagtg tgcaaatggt acgaagccgg
     2041 cagcgatgga taatgtggca agaatcaata aaggaaactt cattgaccag gcaccttcac
     2101 catggaaatg atggagatga atatccgcct tattccagaa aatgcggaaa tacaatctga
     2161 acatataaaa tgcagtcaac ccgctggtaa caagtgccac ataatagata tcgggatgcg
     2221 tggtatatgc ggcatgcaaa ataccttcct tgctgaaaaa tccggcaaat ggaggcacgc
     2281 cggcaatggc aagacaacca atcaggaagg tgatatgtgt gataggtaaa tattttctca
     2341 atccacccat gtctttcatg tcattgctat gcacggcatg gattacaagt ccggcaccga
     2401 ggaacaacaa tgatttgaaa aatgcatgtg tgaacaaatg aaacatacta gccgtaaatc
     2461 ctgcatcatg cgcttgtgaa ctcacgccaa gtgcaaacat catgtagccg atctgactca
     2521 tggtggaata tgccaacaca cgtttgatat ccgtctgtgt gcaagcgatt aaagctgcaa
     2581 acagggcaga gaaaattccg atataggcaa caacatcaat tgctcccgat ccggtaatgg
     2641 cgaaaacagg aaataaacgc gctacaagat acacacctgc aacaaccatg gtagctgcgt
     2701 gtatcaatgc agacaccggt gtcggacctt ccatggcatc cggcaaccag atgtgtaatg
     2761 gaaacatagc agacttaccg gcaccaccaa taaatatcag caccagtgcc caggttacaa
     2821 cggaaaatcc gaggaatgtt gctgaatatc ctttcagaaa ttcaggactt gtaagattgg
     2881 caatgatatc tgtaaaattg aaagaatgtg cggttacacc tgtgatgaga ataccgatta
     2941 aaaagaaaag atcggcaaag cgcgtaacga tgaatgcttt tttactcgca gcaacagcac
     3001 ttggtttgtc gaaataaaat ccaatcagga ggaaggagga aacacctact aattcccaga
     3061 aaatataaat ctggaataag ttgctcgaca tcaccagacc gagcatagag aaggtaaaca
     3121 attgcaaata ggcaaaaaat gtagcatatc tgctttcacc cttcatgtaa cctgttgagt
     3181 aaatatgtac cattagtgaa acaaaggtta caaccacgag catcatcaca gaaataggat
     3241 ctaataagat gcccatattg atagataagg tatttgtgaa ttgcaaccac tgcatatcga
     3301 caggcaggag ctgaggataa acaccatttg ctgcaccatc gacaataaaa tattgataag
     3361 cggtaaacaa ggataatacc gcagatgtgc cgatcaacaa agtagcgagc catcctgaaa
     3421 gggccgggaa atattttttt cctgccagac cgattaaaac aaaaccggct attggcagga
     3481 ttggaatgag actgatataa tcgtaattgc tcatgtttaa tacttcattt cttcgctatc
     3541 ttcaacgtct attgattgat gattgcgata aatattgata atgatggcaa tggctacggc
     3601 agcttcagca gcagcaattg caataatgaa tatggtgaaa aaaacaccgt ctaacctttc
     3661 aggatagagg tatttgttaa atgatacaaa gttgatattt acgctattga ggataagttc
     3721 aacagccatc agcatggtga tcatattacg gcgtgtgaat aagccgtata caccgatgaa
     3781 aaacaatgca gtgctgataa ataaaatttg ttctattcca atgttgttca tgattcctga
     3841 ggtttagatt ttaatgcaat cacaatacaa cctatcatcg ctgccagcaa taatatactg
     3901 atcacttcga atggcaatgc ataaccggta tcctgataat ccaacatttg atcacctatg
     3961 cgctgtggtg aaacattaaa ctgatcgcca ctgcctccga taaaaccata ttggcgaatc
     4021 agtgaataag taagtgctgc accaagaatc gctgccagcg ccgaaaatat catccgcgta
     4081 tttgtcggac gaggcatgtc cttacccgtt tgctgagtca ggaatataga aaagatgatg
     4141 agcactacga taccgccaac atatacgatc acctgcactg cagccacaaa ttctacatgc
     4201 atccaaaaat acaaccctgc cacaccgatc agcgagaaca ataaaaatat tgcagccctg
     4261 aagatctttc ttgtggtcat tgccagcacg ccattgccaa taataaatgc ggccagtaaa
     4321 taaaatatga tgctataagc gttcactgat aacaattatg ttttttaatt caattgcttt
     4381 tgatagttat gcattatatc actcaacgcc ttccctgatt ttgctgcccg gtttattgag
     4441 caccttggtt agttcggaac gatcgaaaac actgtgctcg aatgaattcg acatggtgat
     4501 ggcgccggtt gggcaagcat caacacacaa attacacatc gtgcacagtt caagatgata
     4561 tacgaatgta tctatcgctt ttttcttttt gccttcaggg gtgatatcaa atttggtgac
     4621 gattttaatg gtgccattcg gacaagcaag ctcacaagca gtacatcctg tgcaggcatg
     4681 ctcattgttt tcatcgtgcg tcattaccac ttcaccccta aatcgttcag ctaatacagg
     4741 cttttgatta ggatattgct gagtgatgat atttttgtga tgcgtgaaat aataacccgt
     4801 tctccgcata cccgtaagca gcgatttcac cccaccaaaa acctctgata tgtatttact
     4861 taaaaacatc tttataattc atttcaaccc aataaactca cccacccgcc tgctcggcga
     4921 ggtaaactca cccactgacc cactttctcc tctctcctct ctcctctctc ctttctcctc
     4981 tctcctctca aaaataccac ccctgaatcg ccaccaatgt cgccaataac aaattaaaca
     5041 tcgatatcgg caacaaatat ttccactcca gattcaacag ctgatcaatg cgcaaacgcg
     5101 ggaatgtcca gcgaaaccac atgatcacga aaatcaggaa aaaggttttt ccaaggaacc
     5161 ataccgacga agggatataa tccattatat gattaaatgc ctcccaattt ccgatatgca
     5221 atggcatcca gcctcccaaa tacaaggtgg cgccaatggc acatacaata aatatattta
     5281 catattcagc aaggaaaaac aatgcgaatt tcattcctga atattctgta tgaaaacctg
     5341 ccgttaattc agattcagct tccgccaggt cgaatggcgc tctgttagtt tctgcagtta
     5401 cggcaataat aaaaatcaca aaagaaataa tcaccggaat atgtccctta aaaatccacc
     5461 agccatcctg ttgcgaatta atgatgctgc tgatcgacat gtcttgcgag aatattacga
     5521 tggtaagaat tgataatcct gcgcttaatt catagctcac aatttgtgct ccgctgcgca
     5581 tagccccaag cagtgaatac ttgttattac tcgcccatcc cgccatcaaa ataccaataa
     5641 cacctattga tgaaacggca ctcacataca gtacaccgat attaacatcc caaatcacaa
     5701 agtctttcgc aaaagcaatc ggcgccaaca ccaacatgga ggctatcatc acaacaaatg
     5761 gtgcaagatt gaataaaaac ttatcagctc cggcaggtgt caaaccttct ttaaccaata
     5821 atttcaaggt gtcggctacc gtttgaaaca tgcccttcgg tccaacgcga ttcggcccca
     5881 gacgcagctg catatatgcc gatactttcc tttccatcat gatcaacacc aaacctaaaa
     5941 ctgcaaacag gccgatacac aaaacaccaa caatcacaaa ttcaatcgta agtgccagcc
     6001 aacttgcgca atgggcattt aaccactgat ctatactgtc tgcaatcgat gctaaactaa
     6061 attccacgtt aataatttat actgttcgtg tcgtgcacta ttttttccgg atgtttattc
     6121 cggagggtaa tttaaaatgt catttagcct gaacctgcgc tcaagctgtt tatctgtcaa
     6181 tatccggtat taccagatct aatgttccca taattgccac cagatcacca attttttgcc
     6241 ccttcgccat atgatctaat gccgaaagat tcgaaaatcc tggtgaacgg aattttattc
     6301 tataaggtgt taaacctcct tcgctaacga tgtatatacc caattctcca cgagctgttt
     6361 ccacgcgtgt gaaaaattct cccttcggta attttaatac agcttttgtt ttcgcgacga
     6421 aatcgccttc aggaatattg tcaattaatt gttcgataat actgatcgac tgcctcattt
     6481 cctccatcct gacaaggtac cttccccagc aatctccggc agtttctatt gcttctgaga
     6541 attgtaattg tgcatagata tcgtaagggt atttttttct gatatcgcat gaaacaccac
     6601 tggcacgggc agttgggccg ctacatccat aggaaatggc gtcttctttt gatatataac
     6661 ccacattttt catgcggttc tggaagatga tattaccggt aaccagttca tcatattccg
     6721 gtagtttgga tttaaaaagg gtaagaaaat cctttacttt tttctggaaa tcaggatgta
     6781 aatcaaacat tacacctccg ggaaccatat aattcattgt caatcgtgca ccgcaagttt
     6841 cctccatgat gtcgttgatc aattcgcgtt ctctgaatgc atggaaaaat ggtgtgatgg
     6901 cacccacatc cattgccagt gctccccacc ataattgatg tgaggcaagc ctggtaagct
     6961 cgtccattaa aactcgtata acctgcgcac gctgtggaac ttcaatctgt aagccttttt
     7021 caactgccaa ggcacatcca tggttgttga tatgagccga caaataatcc atccttgatg
     7081 tagaataaat aaactggcga taactcaggc tctcgcacat tttttcgatg gagcgatgga
     7141 tatagcccaa atgtggttcc acctttttaa tggtttcgcc attcaatgtg atcaccaggt
     7201 gcaatacacc atgtgttgaa gggtgttgag gccctacatt tatcaccagg tcgccggtat
     7261 ctgtttttgc tacttcttgt aacatttacg ctttataatt tcgtcccttg cagggatttc
     7321 attccttccc acaacaatga ataatgctcg ttattagagc ttaatcatat tgataggatc
     7381 ttcataatct ttgcgcatcg gccatccttc ccattcgtca gtaagcaata gccttctgag
     7441 atcagggtga tgaataaacc ttacgccaaa taaatcaaat acctcgcgct ccaacaattc
     7501 tgcggtgcgc cagatatcac ttaatgtctc tatttcaggg ttttcacgat tcaactgaga
     7561 tttgataact acctgatgcc catgtgctgt agattccaga tgatacacca tcgtaagatg
     7621 tgtcttccaa tcaacacagg tcaggcaaaa catgtagtcc atcgacaggt ccggcatgtt
     7681 atgtaaagcc tgcatcaggt cgcggcaaag ctcagaaggg accacagcat tgagaaactc
     7741 acctccctct tcgaaggtaa cctgtggctc caggttggtg atggcggctt gtagttcttc
     7801 gcgtgtcatg ctctgctgat tcgtttatta ttgattgtcg taaaatttct ctatgacgat
     7861 tttttcacgt ttgcctcccg cttgaatctt tgtctgcaag gtaatcaagg catgcagaag
     7921 ggcctccgga cgcggcggac aaccggcaac atacacatct accggaatca cgtggtcggc
     7981 acccttaaca acactatatg tattataaaa gaatggtcct ccactggttg cacaagctcc
     8041 catggcaatc acatatttgg gatcagccat ctgatcgtac aagcgcttaa gcacgggagc
     8101 catcttattt acaatggttc ctgcaataat tattacatcg gcttgtctag gtgttgccct
     8161 tgctacttca aacccaaaac ggctccagtc gtacttggca ctggcggtgc tcatcatttc
     8221 aatggcacag caactggtgc cgaataccaa tggccaaaga gagttacttc ttgcccagtt
     8281 aatcacatcg tcgaggcgac tcacgagtaa acctcctgtg gctgtagggt gtacatcgcc
     8341 ggggaaaggc tgttgttgat atcctggctc ttttacatcc atttcaaagc acctttttta
     8401 tgggcatata aaaagcccat gaataatata aaaaggaata ttaccgcttc ggtgaaagca
     8461 aacatcccca accgctgaac agacactgcc cagggataaa gaaatatgag ttccacatcg
     8521 aaaatcaaaa agataagggc gaaaaggtaa tatcctacgt tgaactgaac ccagttttgc
     8581 cctatggtag gcacaccgca ttcgtaaggt tcgcctttta cggggttttt gctcgctttg
     8641 agcaccactt tggaaaggag gatacccgca gtagagaagg ctaccgctgc catgatcaat
     8701 actaataatg aggccggacc cataggtggg gctaaaatac gccttgatag gtaaaacgac
     8761 aaatgtggtt gtgcaccttc gtgggttttc aaccaaattc ccgtaacgca agccttaaaa
     8821 accttaaacg aaaaaaaatg tttagcggat caacaggtat tacatttcaa gcacccctat
     8881 tattgaggtt gacatttatg gtaactattt gccatttact gctaaaacaa gttcgaccaa
     8941 tctaaatgca ggaatggtgg taaacagcaa gtaaataaaa taatattcag atgaataaaa
     9001 gcaggttccc ataccataat caaactatat attgatatta taaaaaaaat caaaaaataa
     9061 acatcaaaac cacctttcac ttttttcaat ttcatgatat aattccaaaa aaaatccaca
     9121 ggaaatattt aacatttttt gaaagttgac ttaaatcacc aatcttgctg agcaaaatca
     9181 tgctgttccg tatgatataa tgagaagttt acgtctgatt gcgggttcac ttattactcg
     9241 tacttaaatt ttcagacata tgtcacatac agaatctcaa ctgatctccc ccgatgtaaa
     9301 acacggaaat tttaatttca aacgcgatat catggatggc aacgaagcag ccgctaccat
     9361 tgccttcaag accaatgaag tctgcgcaat ctatccgata acaccgtctt cgccaatggg
     9421 tgagtgggcc gatgaatggt cttcaaaagg tgtgatcaat attttcggtc aagtgccaac
     9481 agttatcgaa atgcaaagcg aagcaggggc agccggtgcg attcacggag cgttggccgc
     9541 cggagcactc agcaccacat ttacttgttc gcaaggatta ctgctcatga tccctaacat
     9601 gttcaaaatt gccggtgaac ttactccagc cgttttccat atcgccgcaa gagcagtagc
     9661 aacgcatgca ttatcgattt ttggcgacca tagcgatgtt atggcggcca gagcaacggg
     9721 tttttccatg ttgttcggtt gcaactcaca agaggttatg gatatggcat tgatttcgca
     9781 atctgcaaca ttatctgccc gcgtgccttt catgaatatt ttcgacggct ttcgcacaag
     9841 ccatgaattg agtgaagtag aaattattcc tgatgagatc attcgtgcca tgattaaaat
     9901 ggaagatgta atggctcatc gcagcagggc tatgaatccg gcaaatcctt ttattaaagg
     9961 aacggctcaa aatcctgatg tgtttttcca aggtcgagaa gcttccaata aatattatat
    10021 gcaggtgcct gaagtagtta aggcgaaaat gaaagccttt gcatctctta caggtcgcac
    10081 ttataacttg ttcgattact atggtcaccc tgaagctgaa agcattatca ttattatggg
    10141 ctccggagca ggtcctgtgc aggaaatcgt cgattatctc aatgcaaacg gtggaaaaac
    10201 cggcgttgtg atcgtgcgct tatatcgtcc atttgctata gacgattttg tgcatgcaat
    10261 cccttcaacc gtcacaaaaa ttgctgtcct cgatcgttgt aaagagcccg gtgccattgg
    10321 cgaaccattg tatatggatg taatcaatgc gttgcgcgaa aaggaacgcg atattagaaa
    10381 agtcattggc ggacgctatg gtctgggttc taaagaattc aatgcctcta tggcgaaagc
    10441 ggtttttgat gaattaaatc aaccaaaacc taaaaatcat ttcactatag gtattgacga
    10501 tgatgttacc aatacgagct tgcacttcga tcctgatttc aacctcgaaa aacaatatta
    10561 cttccgcgga ttgttctatg gtcttggtgc cgatggtacc gtgagtgcaa ataaaaattc
    10621 catcaagatc attggtgatc ataccggcga ttttgttcag ggatattttg tatatgattc
    10681 caaaaaatca ggctcgctaa cagtatcaca tctgcgcgcc ggaaaaaatc ctattcgctc
    10741 gagctacctg gtgcaatctg caaattttgt ggcctgccat cattttaatt atctgctgaa
    10801 atatgatgtg ttacgctatg ccgaacatgg cgccgttttc ctgttgaata caccataccc
    10861 tcatgaggaa gtatggcaac atcttcccaa aaaaattcag gaagaaatta ttgaaaaaca
    10921 attgaaattc tttgtgataa atgcaagtgc agtcgcacga caagcagaat tgggcacacg
    10981 cattaatacc attctgcaaa cctgcttctt tgccatcagt aatgtaatga gcagcgatga
    11041 ggctatcgca gatattaaac aggcaattca cgattcctat attaaaaaag gtatctcagt
    11101 agtggagaaa aattatgctg cggtagataa ggcgctcgca aatttgtttg aagtcgacta
    11161 tacaaaaaat gcaaccggaa atctggaagg agataaaatt gttcctgatg atgctcccga
    11221 cttcgttcaa aaagttctcg ctcgaatcat ggctggtgaa ggcgatgaat tacctgttag
    11281 tgcttttcct gtagacggta gatatcccgc aggaacaaca caatatgaaa aaagaaatat
    11341 cgccgagcaa gttccggtat gggacgcaga cctctgctca caatgcggaa aatgttttat
    11401 gatttgtccg catgcagcca tcagaccaaa agtgtatgct aaagaagcac ttgctgatgc
    11461 accgccaact ttccgacatg tagcaccaat tggtaaagaa tttaacaagg aggaagaagc
    11521 atataccttg caagtaagtg ttgaagactg caccggatgt aatttgtgcg ttgcctattg
    11581 ccctgttgaa agcaaacttg aaaaaggaca caaggctatt aatatggttg acaaacttgg
    11641 tcttgaagaa agtgaacagg ccaattggga tttcttcctc tcacttccag acatagatcg
    11701 agaaaaagta aataaaaata cagtcaaagg cactcaattc ttgcaaccat tattcgaatt
    11761 ttcaggtgct tgtgctggtt gcggcgaaac accatatttg aaattgctga ctcaattatt
    11821 cggcgataga atggtagtgg caaatgcaac cggttgttct tcaattttcg gcggtaactt
    11881 gccaacaaca ccttggacta ccaatcatga aggtcaagga ccggcttggg caaattcgtt
    11941 gtttgaagac aatgccgaat tcggattggg tataaagttg gctacagata aaaaacgtga
    12001 aatagcctta tacctcctcg accaaatgca accaatgatt gatgcaggat tatatgatgc
    12061 cataaaatct catcacggca ctgcagaatc tgatatcaat atatataaac aacaaatcgc
    12121 agcgctaaga aaacaaattc gtggtatcga taatccaaat gcacatatgt tgctgcattt
    12181 cgcagattac ctgattgaaa aatctatttg gataattggt ggcgacggct gggcttatga
    12241 tattggtttt agtggattag atcatgtgtt gtctaccggc gaaaacgtga atattttcgt
    12301 gttagataca gaagtgtatt ccaacaccgg cggtcaaaaa tctaaggcat caccgttagg
    12361 tgcaagtgcg aaattttcta tcaatggtaa acgcagcggt aagaaagacc tagcattgca
    12421 agcgattgct cacggaaaag catatgttgc tcaaattgct atggcggcaa acgatatgca
    12481 taccgtgaga actattcagg aagcagaagc attcccgggt cctagtttaa taattgcgta
    12541 cagtcattgt atcgctcacg gctatgacat gtgccatggc agcgatcaac aaaaaaacgc
    12601 tgtaaattcc ggatattggc cattgttcag atataatccg ctcaaagcaa gaggcgaacg
    12661 atttacaatg gactctaaag acccaagtat accactcagc gaattcctct atcatgaaaa
    12721 tagattcgcc tcaataagag cgaaagatcc cgccctcgca gaaacattcc tgcacatggc
    12781 agaagcggat ttgaaagcca aatatgaacg attgcatcta ttggaatcga tgtgatcgat
    12841 tgtcattatt aatggaatac cttgaaagga gggacgacag ttttattcgt ccttcctttt
    12901 ttaattacaa taagttctga tacctatccc gcacctcact catacagttg ccgaattacc
    12961 caattacagc tagactgact tctattaata agcagttatc aaaactatta tttagtaaat
    13021 aatttaagtc atattgataa attgttaaca taattgcgca ttgttaatca tacatatagc
    13081 aaatatgtcc ttaaactaaa taaaacaata cgttaacaat gagttaacac tactctcggg
    13141 aggtaactct acctttgctc aaaacctatt tgcatgtcaa ctttaaaaaa gactttcttc
    13201 gccttgacac tgctttttgc aacgcagcag ttcgtacagg cacaaacaac cgaacaggct
    13261 ttcagccctt ctatcaaggt gaaagcattg attcactctc ggtttgaggc ttctcttacc
    13321 gatagcatcg atgtagcagg taaatatttt gctaaccctg taacaactaa ttttcgcatg
    13381 cgtcgcattg agctgcgcac agacattaaa ctgaatgata aattttcagg agttgtacgt
    13441 gttcaattac ccgaattgaa aggctccagt gtgggtaaag tgatcgagtt ggcatatatg
    13501 gattataagt ataatgatat gctaaatgtg cgtttcggtc aattcaaatc tcccttcgaa
    13561 cttgatgaac aaaccagtca cgaagacctt cgcatgatcg atcgcggacc aacaagtgtt
    13621 atgttcgtaa ataatagtct cgcatcatac caaccgggcg ccatggtatt tggaacattc
    13681 atgaaatcca aaacaccgct ctcatactat ctcgcttttg ttaatggaag taatcgcgca
    13741 gtaaataatg atgataatag ccagaaaaat attgttggac gacttgaatt cgtgccgatc
    13801 aaaggagtta aaatcggtgc taatgctcag cagatctata caaaggacac cagtggtctt
    13861 tcatatggcg gagatcttag catcattcga gacctaaacg ataaaatgaa attaattgtt
    13921 gaaggtgaat atatcacagg atatcaggta aatgatttta atgctgatac aaccggtctg
    13981 gaaattggcg attatacaat gggtggttat ttcggacaag tattattgcg ctctgaatta
    14041 aataaatcat ggtgtaaaac tttcgaagtg ggtggtaaat acgaacatac tgatccaaat
    14101 accactgttg atgacaatgc atttaatacg ataacaggtg gaattggatt cattttcact
    14161 cctgataata tggctcgctt gcaattgaat atcgtacata cagactacga aaaaacaatt
    14221 actccgggtg gacttatggc tgcaaatatg tttgttacgc agttccaact taagttctaa
    14281 ggcaattaca ttattcaaaa aaagcctgtt tcttccttga aacaggtttt tttcgttggt
    14341 ggttaaacca atcgcacacc cgtctgtcta aatacacact taattacctt taccatctat
    14401 gaacttgaaa tattacatcg ttttgcttat cgcaaacagt cttcaactat atgcctttgc
    14461 acaatctatg cccgaccaaa tggttatttc tgatgatggc accagactgt ctgtcggagg
    14521 tcaggaacca aatgattttt acaatatcga ttcaatcagg tcaattaacc tgatctttga
    14581 cgaacctaat tactggagcg cattaactgc caattatgcc tctaaaacag acctggcagc
    14641 caccatggtt tacaatggtg ataccctggc tgaacaagtt ggtgttcgtt tcaaaggtca
    14701 aaccagttat atgatgaata tgactgataa aaaatcattt aatataacca ttgactatat
    14761 cgatagctcc caggatatcc atgggtataa taccttaaat ctcaattgtg gatatgaaga
    14821 tccttcattc atgcgcgaat cggtttatga aaataatatc aggaagtata ttcccggcct
    14881 tgctgtaaat tatgtgcatc tatatctcaa tggcgaagat tggggactct acctcaatgt
    14941 tcaacagctt aataaagatt attataaaga atggttcatg accaacgatg gcacaagttg
    15001 gcgcgctgaa aaagaaggat tcggcggacc gggtggcggt gatcccttcg gcgcaggata
    15061 ttgctctctc aactatcttg gaactgattc ttccgattat acaccatact accaattaaa
    15121 atcaacagat cttgaagatc cgtggacaga cctgatcaat actaccgatg ttctcaacaa
    15181 cactgctgat gcctctttgg aagacgctgt gaataatgtg cttgaccttg accgcactct
    15241 gtggttcctg gcactcgaaa ttatattcag cgatgatgat agttatgtca ataaaggtgg
    15301 gatggattac tatgtgtatt gggaaaaaga aacagatcgc atagtgccaa tggaatatga
    15361 tggcaatagc tgtatggatc ccatgcattt atcgtggaca ccattttatc atgaaacaga
    15421 tgctgattat gcattattga acgtgctcct cggtaacgat aatattcgtc agcgatatct
    15481 ggctcacatg cgtaccatcc tcgacgaatt attcaactcc gattacatgg atgcctacat
    15541 cgatgcaatg gatacacgca ttgacgcact cgtaaatgcc gatcctaaaa aaatctatac
    15601 ctatgcacaa tacaatgctg atgtcactga actgaaaaca tttgtcgagg aacattataa
    15661 tgatttcatc aataataccg aaatagatcg ccttgaccct gaaatttcag aaacactgat
    15721 gtattccgca gcaggagatt ggattgctcc cttatcaatg gaagcacccg tagtaaccac
    15781 aaatgtgtat actgaaagca atatcaatgg tgtaaatctc tactacggca cagggctagt
    15841 tggaagattt tacccgctgc aaatgtttga cgatggcgct cataatgatg gactttttaa
    15901 cgatggaacc tggggtgcaa atctccccgc tatgcctgca gggactgatg ttcgatacta
    15961 cattgaggca attgccgatg atggatacgg aacacgctcc tatgaccctg taggcgctga
    16021 acacgatgta tattattatc aagttcgagg caatgtggcc gtagaagact ttattgcaga
    16081 aactattaat gtttatccga accctgccac tgatatggtg caaattattt tgccgtcatc
    16141 aaatgaaaca gctgatatcg aattgttctc tcttggagga aatagtgtgc tgaatcaatt
    16201 aaattataag tcaggagcac ctctcaatat atctaaaatt gctccgggtt gttatttcct
    16261 gcatacaaaa caaaatgaag ttacagcaat aacaaaaatc ataattcagt aaaaaagaaa
    16321 aagccatccg aaactcgaat ggcttatttt ataagtgtta tctgtttaca aatctgcttc
    16381 agcggcttgg attaccgttt tgttttctgt ttcgaaatta tgcacaaatc ctatcactgc
    16441 catattatcg gcattccatg tgtcaggaag cgtgtagtcg tattcgaatg tataccatct
    16501 gccggcaaca ttttcttccg gcaaagcctc accaaggtaa aaacttagga attttctcaa
    16561 tacatgattg tgtacgtaat cctcaacctc accgccaaca ttatcatcga tctgcgctgc
    16621 aataatgcca ctttcaacta atgcaacact taaatgattt acattcgtaa catcagaagt
    16681 ataggctacc ttaaccagca catgtaagtt gcgtgttgcg gcatcaaacg ttggggttat
    16741 ttcaatatta catggagagc taagagctaa ttgatcattc acatacgaag caatattcgc
    16801 aggcacttca ggaatatatc catcggcttc aaaatctgtt ctgtcaataa atgaggcagg
    16861 ccatcctaca accggaccca tgtaatcatc aagttgatat gattctgtag tggtcagatt
    16921 ttgctcgata tatagtggat ctgcaaaata attatactct gctattgctg tcacccttcc
    16981 aggatgtgcg cttattgcat cactaatagc aatatgtgcg tcggggcaat tcggacatgc
    17041 agctcctgta aaatcttcta ttaacactac tttgttttga ggcgtttcag gagtgccaac
    17101 accggtttca tattctgtat ctgtaaggtc aattgtaggt cccacctcgt cgcacgacga
    17161 aaatgcaaaa ggcaataaag ctaccgaaag cattaatacg tttatcctcg ttctgttcat
    17221 catacaaata aatttagaat gttgttgtaa tacctgcttt taatccgctg aaggctggct
    17281 cgtaacgaca aacacctccg gtgcaaacaa tacccgcaat ttgtttcacg taattcaacg
    17341 tgaatcgggt tgatttttgt gtgtatcctg caaaaacaga ataataatgt aatgcatcat
    17401 ctgttttcaa tggatcgtaa ttccacatgt cgcttacggc aaaactccat ttgggggcaa
    17461 tattatattc aactaatcca tacacccagc taccaaagtc ttgttcgcaa tgctgatatt
    17521 gcaattccaa gcgcatcgat tttttctttg agaatttata agtaaattct gcaaatggtg
    17581 taatcgcctc taccattgga cgcccgggct cgttctcata aattgcctga ttgtagtgaa
    17641 ctaattgtcc tcctaataat aacttgtatt tctgcttgcg catttcgaaa tctgcaaata
    17701 cctccgtaaa taattcggta gtatcgttgg taacattcga ataattgatg ttgatggtat
    17761 atcctttttt cggagtgaaa gtgccattca cctgataagc aaattctccc aactcctgcg
    17821 ccaccgcctg gtatcgtgcc gtaaggcgaa gtgaattctg cttcgtcaat gaaggcaaat
    17881 aatccattat cccatccagt agcgtagtgt tcggagatac tcgcattacc caattatcaa
    17941 ccatgcgcac ttgcccggta ataccaaaac cgtcaccgat ttttgaagtc gagtaggtga
    18001 gtgacgagta taacacattc cccttgctgt catcaagaat cccacttggt gttctgatag
    18061 catcagtcga tttcatcgca tattctgcat accaggtaac actctttata gataatgtgt
    18121 tgaaaatgct ccccgcatac atattgtatt tcggaacaaa cctgtcggca agatcataac
    18181 tgttgatttg cgcaacaatg atatccatat tatcctgatc tatcgttcgg ttaacaaaac
    18241 ttgctcccgg cgctatcgtt atattcgaat tttttgtggg caaaagacct tcaatcgttg
    18301 cacctttaat aacaggctca tatctggtaa acaaatcttt catttgtccg ccaattgcct
    18361 ttattttcca atcatcattt atctgatatt caagttcaac gccaaaaagt gcattgtcta
    18421 tgcctaatgg tctttcttcg taagcacgaa atgtcaatcc ggttccaaat tgatcataaa
    18481 tatatcctcc cgttatctta aatgcatcga tctttttact gatataccac ctgccaatac
    18541 cggcatctgt atatggaatc ccgggattat gaagattcga attcaaaaac atatccaggc
    18601 gaacgcctgc ctccaatccc caggcatcat tcctgtaata ggtagtaagc cagctattgg
    18661 caccactcaa taaattgtcg tagtggggtg taccggtagc cccaattaga ctgtcgcgct
    18721 gatagaattc cgtattcaat tgcaaatcgc cggatatcgc tccctgtcca aatacagtca
    18781 tcgcagaggc aatcatcaag gctactaaaa aactttttct cataggctaa ggtttccgac
    18841 gctaagttaa gcacctgtgc cgactttgat ctccctcctt ttcaaattga caggctcctg
    18901 tagtgtaaaa tgctggatac cggtaatgtt cagacttcgt cgttttaatg tcatttcctc
    18961 ggtcattgcc cgcaaggagg ctaaaaccta aggaaaattg gcacatatct tgctctgcaa
    19021 atgctatctt tgaaacaaaa tatacccttg cgatgaaaaa atttgttctg ctttctgcca
    19081 ccttgtttgc tgctattact atggttaatg ctcaggataa actcccaagc cttgaacttt
    19141 ctgatgttaa tggcaataaa atcaatgtgg ccgattatag taatgacagc actattcagg
    19201 tgttcagctt ctgggctacc tggtgtgtgc cttgtaagga ggagttgaat aatattgctg
    19261 aaatttatga agactggcaa aaagattaca aggttgaaat catcgcagtt tctatcgacg
    19321 atgccaaaac caaggcaaat gcaaaatctt atgccgaagg acaaggttgg acttatacag
    19381 tgcttctcga taccaataaa gaccttcagc gtttattggg cggtcaatcc gtgccattta
    19441 ccgtgatcac cgataaaagc gggaatatcg ttgataaaca taccggctat atcgagggtg
    19501 atgaatatga attggaagat aaaatcgcca aactcgccaa agaataaggc agcttcttta
    19561 gcaccattca attattttta gtcaacaaaa aacaaatcgc tgatcacact gtcggctatg
    19621 aataagtgtg atggagaaat acgtgcaata ttattttcaa taatcatttt ctctgcactt
    19681 tccaatcggc gaatttcatt tgtcagttga ttatgatata cttcaccaaa ttcgctcaaa
    19741 aaccgctcca ggtctaagcc acgtgttgtt cgcagggctg tcatgatccg ctcgttcatg
    19801 cgctgatgca tgtctaaatt ttctatggtg tagtattcgt ttcccgattt atcgtatcgt
    19861 atatataagg cattgttgtt gatattccat tgtcgcgaaa caccattata cgaatgtgct
    19921 gcagctccaa ttcctaaata ataatatcct gcccaatagg cgctattgtg tatggcagta
    19981 tgaccgggta atgcaaagtt ggaaatttca taatgatcat atcccgcatc aatcaacatg
    20041 tggcgaagaa tctcaaattg ctctgcagcc aattgctcat caggcatttc ggattttccg
    20101 gtttgaatga aatgggcaag agcagtcttt ggttcaacgg tcaacgcata gctcgaaata
    20161 tgtggcacct tcaatgctat caatgtttga atattggagc gccattcctc atgcgatgat
    20221 ccggggatac cataaatgag gtctgccgac atatgctcga acccacttaa catggcatcg
    20281 gtaataacct gctttgcctg tgaccccgta tgagtgcgat tcatccattg aaggtcgtgg
    20341 tcaataaatg attgtacgcc aatgcttaat cgattaatac cggctgcatg aatggactca
    20401 atatagggtt tagaaagatc atcgggattc gcctccagtg taacctccgc atcagcgcga
    20461 acggtatgga gctgcttgat atgtgcaagc agggaactga tttcatcagc cgtcaacaca
    20521 gagggtgtgc ctcctccaaa gtagatcgat cgaatgatct ttccttcggt ataccctaca
    20581 cgcgcatcta tctcactgtg catggcaagg aggtatgcct cttttgactg cagggaggtt
    20641 gagaaatgaa aattgcaata atgacaggcc tttctgcaaa aagggatatg caaataaata
    20701 ccggcctcct gatcgttaat gtacatgcgc ttactgcaaa agtatgggta aatttgcata
    20761 accattacca tgaggcgatt cggtatactc ctctgcgtga atttttttct ttggcatcat
    20821 gcacatgctc aggatagtat atatattgtc acaaactaca acaatcaact taacgaatat
    20881 ctgtccgatt tcaataccac ttttgctact atacaggaag cagaacatgc attgaatgat
    20941 ttggtgcaca cgatgcaaat agacggctac ctcactgctg catggcgatg ggaaccaaat
    21001 gaaatgcacc gtaacccttc tgtaaaagtc gtagaaataa accttggcaa aaaagtgctg
    21061 tgggctaatc tggatgcttc aggtgtagag ccttggcttt tgcgcgctgc aggttttgat
    21121 cccgaagatt ggataaatac tacttataat caaaataagg cagcatcatt tttacaggaa
    21181 ttggtatcct atgcaaacaa caatggttat ccgtttgcat catcgcatct cgacagtata
    21241 acatacatcg actcaacatt atcggcaaag gtgatatttg agaaaaatat tctcatcttt
    21301 tttgatacca ttaaggtaat aggatcatta caagtaacat caaactttat cacacaatat
    21361 actcaaataa tttccggtca accttatgat caatcactga tcgatgcact gccttcgcgt
    21421 ttgaaagaac tgaattttct tcagcaaaca aaagcccccc aaattgagtt catgggaaat
    21481 aaagccactg ttctcatatt cgctgatgac aagcgttccg gcagttttga ctttcttctt
    21541 ggtgtgctgc cgaataatga aattacagga cgattgatca ttacgggtga tatgcgttta
    21601 catcttcaaa acgtgtttca ttcaggcgag gagattcatt tccaatatac aaaactcgaa
    21661 agtgcttcaa agtcaatgga cgcggcattt acctatccat atttaccgca tataccgctt
    21721 ggccctgatg cctcgtttca tctgtattta aaggattcga cattcctcga aagaaaagct
    21781 tctgcaggcg ttgtttggca gatagcagga aatcattcat taaaaggttt tacttcgttt
    21841 tataattctt ccgtactcag accggatacc acttttgtat tgttgaatta tgcattgcca
    21901 tctatgctcg acgtgaaaga gaatgcttat ggcctcacct ggcaatatca acatctcgat
    21961 tatacattta atccgcgaaa gggatacgcc attgcattga caggcaccgc cggaacgcgg
    22021 actatcctcg aaaatgtaag cattacagaa ttgtttaatc ccttaactcc tgatttcgat
    22081 ttcagtacat tatatgatag cattgaaaat aaatccctgt cgctgaaata tcaatatgat
    22141 ctgcaatatt ttattccctt gtataatcgt atggcgattt tattacaggt gcgcggagca
    22201 tcgatcttac acgataatat tgttgagaat gaattatata gaatcggcgg aaatcaattg
    22261 ctaagaggtt ttgatgaaca aagcttgccg gtctccgact atcatatcgg caccttcgaa
    22321 tatcgatacc tgctgtctca aaatgcctat acatcggttt ttgtcgatgc cggcgttacc
    22381 aaaaataatg cactcgataa tactgctttg gtctatccct acggtttcgg cgccggcgtt
    22441 cgatttgaaa caaaagcggg tatctttggc ctttcatatg cactcggagg ctcaaatatg
    22501 aatcctgtgc aggtgaaaaa tacaaaagtg catttcggat acatcaatta tttctagtga
    22561 agcgaattac ggtagctgca tgcttaatgg tctccctcag tgttacagcc ggggcacagg
    22621 aaaccatttt gcaaaatccc gctcccgata ccctcattca ctacgcttat gatactgctg
    22681 ctttgatgaa acgcatgcat tttgcaccgg ttgattacga tagcctcctc gcatcaccaa
    22741 acaccaatcg caaaacagca cctttcagca gctttcatgt cgaaaaacgc gatcttctgc
    22801 ttcccggatt gtgcctgctg atgctaattt atgttacctg gctaagatat acctatggca
    22861 aggaattgcg tgagaatttt actgtcatat taaataccaa cctcggacaa cagatttacc
    22921 gcgatagaga gttttcggcc aacattttta agtttctcac cttcatcaat tttgccctgg
    22981 gaggaggtat gctggtctac ttgaccacaa catattatca tgtgaacctt ccctttaacc
    23041 agacagggtt aaatgtcgca cttatggtta tggctgtatt tgcattatat ctggtgaagg
    23101 gctttgttta taggtttgtt ggtgctgtat tcaatatcac ggcatccctt agatttttca
    23161 gattcaatgc tttggtgatt taccacctgc ttggaatagg actgcttccc ttcatccttc
    23221 tggctgcctt tgcagaacct cctgtcaata cctgggcact gtttgcagct ttgatattgg
    23281 ttgccattgc cattgttttg agggtgttta aaggccttac ggttgccctg agttcgggga
    23341 aattgcatat cctctacttt ttgctgtata tttgcgccct cgaagtggcc ccactactca
    23401 tagccataag gctgtttgac ctttgggccg gttcacagcc taaataagta accgatgaac
    23461 aaaccgcagc caaccagttt gaaaccggta aaatctattc tcgtttccca gccaaaaccg
    23521 cctgaggtgc ctaaatcacc atatcaggag attgctgaaa aatataagat taaagttgag
    23581 ttccgtcagt ttattcaggt gcgcggactg tcgtcgaaag acttcagaaa gcaacgtatc
    23641 aacatactcg attacacagc agtcattttc accagccgca atgctatcga tcatttcttc
    23701 cgcatttgtg aagaactgag aatacgcatg ccggaaatga caaaatattt ttgcgttacc
    23761 gaggctattg ccgtttacct tcagaaatat atacaatatc gtaaacgcaa ggtattcttc
    23821 ggcacaggaa aagacacgga tatgttcgat ctgttcaaaa aacatatcag cgaaaagttc
    23881 ctctttcctt gtacagtcaa ccacaaagac atctataccg aatatctcaa atctattaat
    23941 gccgatttct cagaagcatt catgtatgaa acggttcccg ccgatctttc tgatttgaaa
    24001 gggaaaatag attatgatat gatcatcctc tatactccaa ccggtgttgc aagcctcttt
    24061 caaaacttcc ccggattcga gcaaggcgat ttgcgcattg gttgtatggg cgcatccaca
    24121 ctcaaggcta tggaagaaca aaatcttcgc gtagatctca aagcgcctag tcatgattct
    24181 ccttccatta ctatggcatt ggataaatat ctcgccatca gtaataaaaa gaagtaagta
    24241 atttattcca acactccttg cctttacatg cgaacctatt cgaataggtc agcttgctta
    24301 ttttttatgg ccgatgtaac tttaatttct taggaattaa cggcatacaa actttgtaaa
    24361 tacctttatt gttaatccga ttgattgtaa ttgccattca tgtgtaagga aggcgaaaag
    24421 tttttattcc tccaacctga gatgcaatcg gaaaacctag taaataccta aattaatatt
    24481 cgtatgccaa caaataaact catcaatgaa acttcgccct atctgctgca acatgcacac
    24541 aatcctgttg attggtatcc atggggtgaa gaagcattag aaaaagcaag aacagaagat
    24601 aaacccattc ttgtttctat cggttacgcg gcctgccatt ggtgtcatgt gatggaacac
    24661 gaaagttttg aagatgatac caccgctgaa ttgatgaata aatactttat caatattaaa
    24721 gtagacagag aagaacgccc cgatctggat aatatttaca tgaatgcctg ccagatactc
    24781 accggtgccg gaggatggcc gctgaacgtt ttccttacgc ctgaattaaa accatttaac
    24841 gccggaacct atttcccccc taagccgggc tatggcaaac cttcttggaa agaattgttg
    24901 atgtatatgt atcaggttta tgctgacgat cgcgataaag tagagcatca ggcaaatctg
    24961 atcatggaac atatcgctaa aatggatggc gcttttctag agaaaaattt tgttttagaa
    25021 tctgaaaata ttttttcgaa acaacaattg gatgctgcta taaacaccat tcagtcaaac
    25081 ttcgataggg tagacggtgg ctttggaagt gcacctaaat tccccgcaac catgactctg
    25141 cgatttttgt tgcgatacgc atggttcaca aataataaac caattctcga tcatgtaatt
    25201 ctttcactca ataaaatgca ccagggcgga atcttcgatc agattggtgg cggattcgca
    25261 agatatgcta ccgataataa atggatgatc ccgcactttg aaaaaatgct ttacgataat
    25321 gcactcctat gcagtcttta tgccgaagcg tatagggcta ccggagataa taattatttg
    25381 caggtagtga aagacattat tcaatatatc gatcgagaat gtagcgatga acttggcggt
    25441 ctttatgctg catatgatgc cgatagcgaa ggagttgaag gactatatta tacattcact
    25501 taccaagacc ttgaaaatgc tttgggtgat gatgccgtat gggcagcgaa agtgtttaat
    25561 gctacctcag cggggaattg ggaacatacg aatatacttc accgcgatgc ctcagatgaa
    25621 acaactgcgc gacaattgga tatgacgcac gaagcattct cggcaaagct taagcttgta
    25681 aaaggtaaat tattgcaata tcgtgaaaag cgaatcaagc ccgcacttga caataaaatt
    25741 cttcttagct ggaatgcatt gatgtgtagc gcatttgtag acgcatataa ggcttccggc
    25801 gaagagcaat tcagacaaaa agcgatacaa cttgttcaac atttaacaag tgctttcaaa
    25861 agaaacaata ctgaaaatgc atactggcat gtcatcacca acaacattgc aaaacaaaca
    25921 gcttttcttg acgattatgc tacattaatt caagcattat ttgatgtgtt tgaaattacc
    25981 ggcgatgaag catggtttaa acaagctgat ggctgtatgg aatatgtgat tgcaaatttt
    26041 agtgccaccg atggaatgtt tttcttcacg gagaaatcgc aaacagattt gccttcgaga
    26101 aatgctgagt attatgataa tgctacacca tccggtaata gtattatgct ttcaaatttt
    26161 cagagaagat caattatgtt aggaaatact aaagatgtag aaagggtccg gacaatgctg
    26221 ggtgctatga aaaattcatt aataaaatac cctacttcat ttggtaattg gtggctgtcg
    26281 gcattgaaac agatttaccc ttcagttgaa attgcagtaa cgggacccga ccatcaagtg
    26341 atgcgcgata gaatttttac acattatcat ccgcatattt tggtacagaa cggcaacaat
    26401 gcagtatccg aattccccct gctccaacac agagcaagtg atgtcgagac cagaatattt
    26461 gtttgtcaca atttcacctg taaaaagccg gtcaccacat tgcaagattt tgtttctgag
    26521 gtgcagacac ttggataagc gtacagtgtg tagtaccttt atcagcgcat acggaaatat
    26581 catattttac gttatagttg caatgaagca gatctacgca agaattacat gtggcttggt
    26641 tttcagtgtt gctgtgttta acgcacaggc gcagttgatc agctacggtc agcaagatcc
    26701 gcaattcacc caatacatgc ttaatacctc cggattcaac ccggctgcca ttggtgtaga
    26761 ggacgcttgg gtttcaaccc ttggtgtgcg tagtcagtgg gtaagtttac ccggagcgcc
    26821 cgtgtcacaa tctttaactt ctcacgcacc aatttataaa atcaatagtg cggttggcgt
    26881 gacagtgatt aatgacatta tgggacagca acgtacaacg ggggcatcac tggattatgc
    26941 gtatcacaag cgttttgggc cgtcgacatt gagtattggg gctagtggcg gtatttttca
    27001 ggagacaata aatggtgcaa aattggtaac gccaacaggc aattatgagg gtggtgtgaa
    27061 ccataatgac cctaatttgc ctgtcacaac tatttcaaaa atgatacctg atgccgcctt
    27121 tggtatgtat ttctttggcc ggaatttgac tgcggggata tcagcaaacc atttactgct
    27181 gccgattaca gcgggaaacg atgcaaattc gggcacagta caatataatc ccaacggata
    27241 cgtttatgtc gcatatggtt ttgatgcggg taaccggttc cgtattatgc caaatgttct
    27301 ttataaaacc gatctcaatg aaggtgttgt cgacctcaac acccttgtca catacaacaa
    27361 caacatccaa gcaggaatat cctgcagagc caatacagca ctggctttcg atgcggttgc
    27421 actgatggcg gggatgaggg ttgctgatcg ctggttactc agctacagct atgatgtcac
    27481 cttgtcggct ttgaacacag ttagttcagg gtcgcatgag atagtagtaa gatacagcat
    27541 tccggtggcc agaccgcgtg cagggaagat gatcaacaac cccagatttc tgtatcatta
    27601 acaaccaaaa atttgtgatt tggtaaaaag tggctaattt cggcctactt ttacccaatc
    27661 tgtgtgtata aacagtggta acacaacttt gtattacgtt aaaaatgcta tttttacacg
    27721 gaataatcag cacaaattga tctcaagtat gaaaagaaat ctgcaattgt tcacccttgc
    27781 attcgtggcc atcatcgttg gctcaggctg tagtcattcg agttgggatg attaccgcgg
    27841 tcaactcctc ggcatgcagg accgtccgga atgggagccc gttaatccat acgggatggt
    27901 gtacattccg tcgggaaccc ttcacatcgg tccgagcgat caggatgtga acaactcact
    27961 atgggcacga gcgaagtcca tctctatcca gggcttctac atggatgatg ccgaaatcac
    28021 taacaacgag taccgtcagt tcgtttactg ggtgcgcgac tctatcatgc acacactcct
    28081 cgaccactat gtggaagaag aagaaggcat ggaatcagag gtgcctctga tcgactggag
    28141 tcaggaaatc gactgggacg atgaggaaac acgctcacgc ctggaagaac tttacctgcc
    28201 tgccagcgaa acttactatg gtattaaggc aatcgacact cgtcagttgc agtataacta
    28261 tcgttatgtg aagtggaaag aggctgcaag caaatcaaac cgcgataaaa acatcgctga
    28321 gttcattgtt gaagagccga tagaaatcta tccggataca ttggtatggg tgcatgatta
    28381 cacctattca tataatgagc cgatgactcg tcagtatttc tctcaccctg cattcgatga
    28441 ttatcctgta gttggggtgt catggaaaca agcaggtgcc ttcgctacat ggcgttcaga
    28501 tttctggaac gactggcgca gagcacatga agaagtgcaa gtcgacgatt tccgtttgcc
    28561 tacagaacac gaatgggaat acgctgcccg cggtggtcgc gatatggcac catacccttg
    28621 gggtggtcct tatgtgcgca ataccaaagg ttgctacctc gccaacttta aacccggtcg
    28681 cggtaactat cctgaagacg gtggctacta taccgtgaaa gtatattcat atgctccgaa
    28741 cgatttcggt ttatataata tggcaggtaa cgtttctgaa tggacaagca gcgccttcca
    28801 ggaaaatact tattcattcc tccacgatga gaaccctgac tatcgttatg atgcagagga
    28861 cagcgatcct cctgctttga aacgcaaggt actccgcggt ggctcatgga aagatatcag
    28921 ctattatata cagaccggct ctcgtcactg ggaattccag gatactgcaa aatgttatat
    28981 cggcttccgt tgctgtatga cattcctcgg tcgttcaatg agtgattttt aatctgatta
    29041 cataattccc gataagccta acttttttat ttagtacttc aatcatttct taaaaccgtt
    29101 aaaaaacaaa agcaaaatgt cagcattttt tgaatccgat cgttttaaac gaatcaaaaa
    29161 cctgatcatc ggtattggag catccgtagt attggtgggt gcattattca agatccttca
    29221 ctggccgggt gccgaccaga tgttgatggt tggtatgctt accgaggcat tcctcttcct
    29281 catgctgggt attatcccac cacacaaaga ctattattgg gaaaaaatct atccggacct
    29341 ggatatcgct cctgaagttg aagaagctaa atccggaaag aaagtacaca aaatgactga
    29401 gccgaaagaa agcgtaactc gtcagctcga caaaatgttg gaagacagca aagttgagcc
    29461 ggtgatgatc gaacgcctcg gtgaaaacct gaaaaaactc ggtgaaaaca tcgagaaact
    29521 ttcagatgtt caggatgctg ccgtaaatac gaatgagtat tctaacaaaa ttaaagatgc
    29581 ttcaacagct gttgctgaaa tgcgcgatgc ttatgcaaat gcagctcgtg ctatggaaca
    29641 gattactgca tcttcagttg atacatctaa atatcatgag caagtgcaaa tggttactaa
    29701 aaaccttagc cagctcaatg ctatttatga actcgaactt caggatacaa ataaccacct
    29761 gaaagcaatg aacaaattct atggcagcct taactctgca atggataacc tgcaggaatc
    29821 acttaccgat acttctaaat atcgtgaaga gatcgctcag ctttctaata acctcgcagc
    29881 gctcaaccgc gtgtatggta atatgttaag cgcaatgagc atgggtgctg cccgttcata
    29941 aaaacaatta atgatcaact tttacttaac taataaaatt taataaacaa tgtcaatacc
    30001 taaggaacca cgccagc
//
LOCUS       DAOQOU010000019        29448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_705_length_29448_cov_5.355731, whole genome shotgun sequence.
ACCESSION   DAOQOU010000019 DAOQOU010000000
VERSION     DAOQOU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 29448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29448
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_29448_cov_5.355731"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLM90_03040"
     CDS             complement(<1..529)
                     /locus_tag="PLM90_03040"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88373.1"
                     /translation="MNQYKVLTVTHKQVPLGQLSNYIVPFDKQTTNYSQQLQSIKQEM
                     KLDELMYLNTCNRVTYFFTTTQPVDNQFTAGFFKTVNPEFEACNVMHSQLFEGVEAIE
                     HMCEVSASLDSLVVGEREIIRQLRVAYEESNEMALTGDDIRLAMKMLIPAAKKIYTET
                     HIAEKPVSVVSLAALR"
     gene            complement(655..1107)
                     /locus_tag="PLM90_03045"
     CDS             complement(655..1107)
                     /locus_tag="PLM90_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88374.1"
                     /translation="MRTSAYIIIACMLLSACKKDEEINPPISEVPEIELMSVSPGTVV
                     ALEDSIVFLIHYIDGDGDLGYNEPDSMSLYITDNRIPLTESFFVPWLAPEGSEITIEG
                     DLEVQLHNTILVDPDSDSETVTFTVWMRDRAGNLSNSVTTGPITVTRD"
     gene            complement(1110..2090)
                     /locus_tag="PLM90_03050"
     CDS             complement(1110..2090)
                     /locus_tag="PLM90_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88375.1"
                     /translation="MKQFPITKIAVMSMLCAFTLIACREIIRDEPDLPDNPYDDVDYG
                     NGGTDPVLIDSASFLGLHTFIFSTTCAVPGCHDGTFEPDFRTVQSAYNSLVYAPVIKN
                     DDDDTFTYRVVPGDTALSWLHERITTDDAVLGRMPLYDTLYPHEREMITQWILNGAPD
                     IFGNSPILPDYQPSTYGYICYENDTTGLRLDTNRADLLQPVILPQHSNVEYWFGIYDT
                     DESGVYQLPSWLEQYNIRISQDMLMNTGFTEYTFELEPDFDPYYGPAYYDPGIPTPYW
                     HHFTLNTDDFETGKLYFMRLYLKDTAHDFVTELPDDSQIYIAYYFTFRIE"
     gene            complement(2087..3415)
                     /locus_tag="PLM90_03055"
     CDS             complement(2087..3415)
                     /locus_tag="PLM90_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88376.1"
                     /translation="MNRRKFIRNSLLTTAGMIAAPYILPAGTLSRSSGMRLANHVVLV
                     VFGGGIRNQESVSQQYLLNQAAGPGGNIMPNMLSGAQPTYNLVYDQWAPALASPLATK
                     GTLFKHMRYENGPTGHFNGHTAVITGNYTPDSLNLSANPAMPTLFEYYRKHNDPLKSA
                     INAWWMSIDLGPYVYLNYSKDPLYGANYGANYMSPITTFGNIGVDYFSTLNTMHPDEI
                     TRMNAVRSFLDANFDRSVLDVPGIRNSEEDKQAIEDFMLGLLHGEIPIEWPLPAGVGF
                     DQMTGDLTNIAASWKVMQQFQPELMVINTTNLDICHTNFSSYISYLHRADYGIGWLWD
                     KIQSDPILANDTVMICVPEHGRNTVANTIPDANGLFAYDHTGDENSRDIFCLVVGPPS
                     IVNQGLVVGSEEEPVGQTIDVVPTIAHILGFKDNIPSGYLPGTVLEQAFV"
     gene            complement(3430..3954)
                     /locus_tag="PLM90_03060"
     CDS             complement(3430..3954)
                     /locus_tag="PLM90_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88377.1"
                     /translation="MQASKLFSTLGILGVVLLSNSCKKDVIQEVVYDNIIYGVDTVPV
                     YQSNEEKDRVKTPLQYISGLYSNLYFSSIPGNILDNLVIYRLSIGDKTLVNELILNAM
                     LQDPAVLANIPSDADMRGDVDQFVEDCYLRFYLRKPTAYEQYALSDMIELDADMTVVD
                     VYRAFLLSNEYMFY"
     gene            complement(3941..4828)
                     /locus_tag="PLM90_03065"
     CDS             complement(3941..4828)
                     /locus_tag="PLM90_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88378.1"
                     /translation="MRQGLILISSVYLLAVSCAKDEVIHENLIIDGNIAPPVTGVSEI
                     ALNNYINSLYIDLFGRTATADELSEHAETLQSEQYSIEAREELVNSLLSDWEHINNIH
                     LTTGQKFLVDVDSFAIASQIAYWDYLIDLFTDMGMEGEAGYYIFENDRLIKLITAHID
                     LANGTIGADEFYRRYINNYFYDQVNMGSLNFVVSCFTNLMQRYPTDDEQESGINMVDG
                     VSDVLFLQDGSSKGDFIDIVTSTPEFFQGLVIESFNTYLARDPNSLEMDTYTQQMMLE
                     NNVNGVALIILTSTEYAGF"
     gene            complement(5182..5844)
                     /locus_tag="PLM90_03070"
     CDS             complement(5182..5844)
                     /locus_tag="PLM90_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ88379.1"
                     /translation="MMKKIHLAFLAISIPIFAQAQFTEGDWLLGGSLLLSNTTSNSSG
                     TGYDDKGPSSSTIYAGPAVGYFIGDKWMIGLRAAFNNSITTIYHPDDPALDMHFTRSS
                     VGGGPVARYYPFQSQYVDLWLGAGVYVGFGKNTDESIVFDDNGVGSINEQTDNLINYS
                     LGIGPGASIIFNDHWRLELACGSLGFSGYQTKDKTTDAVFTQQSISLSLGSIGFGIAY
                     KF"
     gene            complement(5859..6827)
                     /locus_tag="PLM90_03075"
     CDS             complement(5859..6827)
                     /locus_tag="PLM90_03075"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HMZ88380.1"
                     /translation="MLFRTNKSKIIGVISFGILLMSFYSCTTDITLDIPDPEPQIVVE
                     GSIENGVPPIISLTSTIPFYGEVNFNELENYYVHDAVVTVSDGVNTVELVEYCLSELP
                     DEILPLVAEYLGVELDTAGELAIDVCLYTVADIFTGSPSFVGEEGKTYDLHIEANGQT
                     ITSSTRLLPAVPLDTMYFEPHPDPENDSLMRLYVTLSEPEPQGDFYRYFTRRNSDPFY
                     SGYSSVFDDNLINGQTFDFTLDRGYDPTEDYSMGTYGYFWKGDTVTLKWCHIDFPTYD
                     FWRTLEFDSGTDGPFSSATIAQTNIVGGLGIWCAYGVSYTTQIIPE"
     gene            complement(6855..9221)
                     /locus_tag="PLM90_03080"
     CDS             complement(6855..9221)
                     /locus_tag="PLM90_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88381.1"
                     /translation="MKTGLFGLLMCACSMLFAQGKYTLSGYVKDEGTGEDMIGANVYL
                     QNDPGTGASTNGYGFFSMTLPEGDYNVVISYVGYDNQIEKVSLHNDVRLNVQLSQGKL
                     LEEVVVKAERGDVNIQSTDMGKVELNIEQIKTIPVLFGEVDILKTIQLLPGVGGAGEG
                     NTGFYVRGGGPDQNLILLDEAVVYNTGHLFGFFSVFNSDAIKNVTLVKGGMPSQYGGR
                     LSSVLDVQMKEGNNQYYQAEGGIGLIASRLTIQGPIVKDKSSFIVSGRRTYIDALITP
                     FLNGTDFEGNGYYFYDLNTKVNYTFDDKNRIFASGYFGQDVFNYSAPDGSLNVSVPWG
                     NATGTVRWNHLFNNKLFMNVSAIYNSFNFSVQSDFQDFNFNLYSGIQDWNGKVDFDYY
                     PGSNHHIKFGANYVYHTFTPYSAQGSFDDVDFSTDTLSNKYAHETGIFIQDEFDVTDR
                     LKINIGLRGSYFAQVGPYNDIVYDENGATVIDTIYYAPGEMIAHYSGLEPRFNMRFTI
                     DPESSLKAGITYNNQYIHLVSSSTSTLPTDLWIPSSDIVKPQIGIQYALGYFRNFKDN
                     AYETSIEVYYKDLRNQIEFRDGYVPELGLDIEDNFVFGVGRSYGAEIFLKKAKGKFNG
                     WIGYTLSKTERKFEDLSTGSEWFPTKYDRVHDLEVVAVYNLSDRWDFSSTFVYATGQA
                     TTIVESFYVIGGQVQTDYGPRNGYRLPAYHRLDVSATLHSKPERKYESSWNFSIYNVY
                     SRKNPYFIYTTYEGDFAYGYLDVQAKQVSIFPIIPSVTWNFKFAQQHI"
     gene            complement(9332..10927)
                     /locus_tag="PLM90_03085"
     CDS             complement(9332..10927)
                     /locus_tag="PLM90_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ88382.1"
                     /translation="MMHEVSFAQSQNIWVYFSDKNATPYTIDNPSAFLSEQAIDRRER
                     MLIPIRKSDLPVDPQYISAIKQTGALVRTESRWLNAVSVTVTGPEMIAEISALSFVKQ
                     ITPVIKRHLIDDVSVPQDQAFYRTADITDFVYGGAQNQNHMIEVDFLHSLGFRGQGIT
                     IAVLDGGFEGVNTGAGFESVRAKEQIKGTYNFPDDTDFVYFSSTHGSNVLSIMAVDEP
                     GVYVGSAPDADYYLIRTEVTDSEHVVEEDYWLEGAEYADIIGADVINSSLGYTTFDDT
                     TEDHTYLDMDGNTTVATIAADMAAAAGILVVNSAGNSGDEPWHFIGAPADGDSVFTIG
                     AVDLNGNYAGFSSVGPTVDGRIKPNVVGQGAGTAVMSAEGIVGYGNGTSYSSPLIAGA
                     CASFMSAFPDLSNMDVINIIQQTATNAETPNNQIGYGIPQFGHAYMALKGITLNDDIL
                     HVYPNPVSDVLVVYIQGFEADQAKFDMFDMAGKMIMSTDLLIDAGKMSGIRIEDLDRL
                     ATGLYTIRLTGDIYSESKLIYIQ"
     gene            complement(10978..12675)
                     /locus_tag="PLM90_03090"
     CDS             complement(10978..12675)
                     /locus_tag="PLM90_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ88383.1"
                     /translation="MQRFLLICLICCLSITSFAQQHTDRYWVYFTDKQFNTFSKQSPE
                     QFLSKASLNRREKQHIPISESDLPVSEYYIQSLENIGVQVYVRSKWLNAVSVIMSDDQ
                     MLQVSELPFVNKISRVGRYQILNEQGTPLNDQTFYRKADNTEITGGLGAASNQIEQIN
                     LDTLFDIGLSGDSMVIAVLDAGFFGVDTATLFQSFWDKDQILGYHNFPDNNDQVFNIY
                     SGYHGSWVMSVIAGEVTDLFSGSAPNADLYLYRTEIADSEYVVEEDHWLAAAERADSV
                     GADIINSSLGYTVFDDPTQNHTYEDLDGNTTVVTMAADMAASKGILVCNSAGNEGAGA
                     WHYVSMPSDGDSVLSVGGVDTSGIHVGFSGYGPTFDGRLKPNVVALALGSAVLDPNGY
                     INYLSGTSFSSPLLAGACATLWQAYPEATNMQILHAVEQSAHLYDTPNDSMGYGIPNF
                     GKAYALLDSLFPEIDTTVVDTTVDTTIDTTLSSIAIIPLLDGDIFTVILQSLTNREAT
                     FAVYNLQGALMHQENVYLNAEMPTTVEMGNKHLYASGIYFLTVRSGRYKQSVKVFIE"
     gene            complement(12682..13437)
                     /locus_tag="PLM90_03095"
     CDS             complement(12682..13437)
                     /locus_tag="PLM90_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88384.1"
                     /translation="MFSRSITGLILTAFIFAASMGCDKPLPEPIAPETPYAFSPLKIG
                     NYWEYAVDSIIYHETTGNDTTLWFVREELIDTFYDLTGDLNYVIDRYRRKTDVDAWQL
                     TNTWSVMQVDGKMIRNENNLRFVKLVSPPLNDVQWDGNVYLGGLDDLPYDEECNRLIY
                     YEGWDYIYSNVNENYSIPDFDFENTITVEQFGDSNLIWFDYAKEIYALDVGMVEKTFK
                     HYYTQDITCPDCPWEQRVQCGFSVYMRLTDYQL"
     gene            complement(13585..14694)
                     /gene="mnmA"
                     /locus_tag="PLM90_03100"
     CDS             complement(13585..14694)
                     /gene="mnmA"
                     /locus_tag="PLM90_03100"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMZ88385.1"
                     /translation="MSKHGKILVAMSGGLDSTVTAIMLHEQGYEVVGITMKTWDYASS
                     GGSKKETGCCSLDSINDARMVAVDKGFHHFIVDIREEFGDYVIDNFVDEYLAGRTPNP
                     CVLCNTHIKWEALLKRADQLGCEFIATGHYASVHQNPDNNRYFISKGKDENKDQSYVL
                     WGLSQDCLCRTRFPLGQLHKTEIRQMARDMGYTELANKAESYEICFVPDNDYRGFLKR
                     RVDGLEEKVKGGKFVDKHGKVLGTHQGYPFYTIGQRKGLGIALGEPYFVTEIIPHENV
                     VVLGLEEELNKNGMFVHHLNMLKYENIKEPMRAVTKIRYKNAGAESTLFSEPGDLLRI
                     AFDSNVSAITPGQSAVFYEGNDVIGGGHIVSAFQQ"
     gene            complement(14782..15408)
                     /locus_tag="PLM90_03105"
     CDS             complement(14782..15408)
                     /locus_tag="PLM90_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ88386.1"
                     /translation="MSKNTTKENIFNQALRMFNEQGIEYVGMREIAKELDIRVGNITY
                     YFPTKDDIVAAIVTELADANNTILEAGDDLSMSTFLQMYSETSSNQYKYRCLFVSFVY
                     LMEHNPKVKEVYIPNQEKRYKTIRKNLKLLMQNGYMDASLNDRTLDFLVSSISLMSRF
                     WLSEGRLRFNDAGKRTLFKHYLELFALHLHPYATKQGRRDIDRFIDDL"
     gene            15456..16007
                     /locus_tag="PLM90_03110"
     CDS             15456..16007
                     /locus_tag="PLM90_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ88387.1"
                     /translation="MKTYKLPYTIRNNFGEILHFQSIRTEGSKTFIDVTNEVQPGCGP
                     VMHVHFKQDEYLRVVEGRMGYQIAGGAEQFAGPGESATFHAGQMHRFWNAGDDVLKCE
                     GWISPPNNIIYYLENIYRLQNEGGGEPGGFEAAYLIRKYRSEFDVAAIPGFVKNVIFP
                     IVVFFGKLAGKYRKFADAPPAVR"
     gene            complement(16197..17234)
                     /locus_tag="PLM90_03115"
     CDS             complement(16197..17234)
                     /locus_tag="PLM90_03115"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HMZ88388.1"
                     /translation="MYKTLLKPLFFRFQPETAHHLALGLYKAALWIPGYGWWHDKHYV
                     IERPELEKEVFGIRFKNSIGLAAGFDKNAAFIPLMARLGFGHVEVGTVTPKAQPGNDK
                     PRLFRLPKDEALINRMGFNNEGVEMMVRNLGKLSVAHAREKYRVVIGGNIGKNKVTEN
                     EDAWKDYVICFNALFDHVDYFVINVSSPNTPGLRALQEKEPLKRIISSLQEINQGKKR
                     PKPLLLKIAPDLNDAQLNDIVEITRETGLSGIIATNTTLDRSMLKTPQAAVESIGAGG
                     LSGKPVLSRSNDVLWFLRRALGTDVPLIGVGGVTDKASMQSKFDRGADLVQIYTGMIY
                     EGQGMVRGLLV"
     gene            17450..19315
                     /gene="hscA"
                     /locus_tag="PLM90_03120"
     CDS             17450..19315
                     /gene="hscA"
                     /locus_tag="PLM90_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HMZ88389.1"
                     /translation="MAQISINIKEGTLKREEIIVGIDLGTTNSLIAIIDKETGTPSAL
                     RDADKFAIVPSIIHFADDGTITVGRSAREHLITDPEHTIFSVKRLMGKSYKDIAAYQH
                     TFGYKIIDDDAERLVKIKVREKYYSPIELSALILKELKLRAERLLQCAITKAVITVPA
                     YFNDAQRQATRDAGKLAGLDVLRIINEPTAASLAYGLGNQDKSSHTIAVYDLGGGTFD
                     ISILRITSSENSGDFFEVLSTNGDTFLGGDDIDRAIMDHWLQTQHVDGNLLSATIRQD
                     LRLTAETAKKHLGSHGEFTGNVDKIQCDLTIAELETLITPLLQKTIQCCERALFDANL
                     NVNDIDTVVMVGGSTRTPFIKKSVSAFFNKPVHDEVDPDEVVALGAAVQADILAGNQK
                     DMLLLDITPLSLGIETMGGLMDVIIPRNTKIPTAAGRQYTTSLDGQANLKISVFQGER
                     DLVRDNRKLAEFILSNIPAMPAGLPKIEIVFQINADGILQVRAKELRSGVEQKIEVTP
                     QYGLTEEEMAAMLLDSIKNAKTDMAERAIVEARVEAQNMIDTCERFIEKNNQYLNEQE
                     IAGTKSLIDNLRNTLNSDDKDVIHQQIEILNEFTRPFAERLMDMAISQAMKGKTI"
     gene            complement(19364..19948)
                     /locus_tag="PLM90_03125"
     CDS             complement(19364..19948)
                     /locus_tag="PLM90_03125"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMZ88390.1"
                     /translation="MFTGIIESLGSVSGLQKDAGNLHITVQSEISRDLKVDQSVSHNG
                     VCLTVVHQAGDTHTVTAIDETLIRSNLGNLKIGDTVNLERCLKIDGRLDGHIVQGHVD
                     TTAQLIQVDEQGGSWLLTFRFDAQFDKYIVQKGSISINGISLTVFNVSEGTFQVAIIP
                     YTWEHTNLSQISVSDSVNIEFDIIGKYVEKMMAR"
     gene            complement(20022..>22192)
                     /locus_tag="PLM90_03130"
     CDS             complement(20022..>22192)
                     /locus_tag="PLM90_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ88391.1"
                     /translation="IVVPGYLDPDDRTFCTDTDVFQINTFNGDVTWEDPSCTEGIYNI
                     AILITEYRNGIKMGTILRDMQIFVNKTSNHAPEIAELNDTCVYAGEELNILVTATDPD
                     VLQTITLTATGGPLYIDESPAEFFSTPNEGSVNGTFTWQTICNHVRKEVYEVVFKAED
                     DFQIGFEEIPLVDLETWLITVVAPPPVILDAVPTANEITVTWQDPYICSGADNFLGFS
                     VWRKIGCDSTMFDKCQRGLSGMGYDSLAFVEDAYSYVDLAVDYGQEYAYRIVAEFGER
                     SDIAPDFVYNQVPGAPSDAVCAELKRDIPIINHASVRTTSTTNGSMYVGWYNPISPDL
                     DTTIFTGPYKYELVRYEGFTPTGTETLVTTITAGEFFEITDTSYIDTMLNTAATAYCY
                     RLKFYFTDLGNFELLGVTDPASSVYLTASPGDNKLNLSWNFNVPWLNFYYDVYKETTA
                     GSGDFELLTSTTETSYIDDSLANGSEYCYYVKAYGKYTIESLPDTLINLSQIQCGVPI
                     DNEPPCPPILTVYNICEEDGSIVVDESDLKNDLTWTNPNHSCADDVVKYLIYYASQQG
                     DPLTLLDQNADAFDTTYTHDGLLSLAGCYAVVAVDSFDNNSAFSNIVCVDNCSDYNLP
                     NTFTPNGDGFNDIFTPILPYRFIDHVDMKIFDRWGNVVFTTNDPMLNWDGIDTNTGKN
                     ASEGVYFYTCTVYEITVDGVRPFPQPKEGYIHLYRESKVE"
     assembly_gap    22193..22292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22955..23320)
                     /locus_tag="PLM90_03135"
     CDS             complement(22955..23320)
                     /locus_tag="PLM90_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88392.1"
                     /translation="MTEETSTEVQQTDYQGISVEELLKKAMLQDTMKFDRWEPFISFV
                     SYRSQENKLYAFAITRHTDSTMLISRYALQDSTWKLISSLSGLRGPGVFYHLNVADYD
                     FDGVRDVFITSVTSNGIAL"
     gene            complement(23403..23822)
                     /locus_tag="PLM90_03140"
     CDS             complement(23403..23822)
                     /locus_tag="PLM90_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ88393.1"
                     /translation="MSIETQIQQYISSQPEAKRNDLLALHKSILYIKPKAKLWFLDGK
                     NEAGKVVSNPNIGYGLLQLKYADGSAKDFYQVGLSANTSGISVYIMGMADKKYLADTY
                     AQAIGKATITGYCIKFKALKDINMDTLQHAIKDGFAA"
     gene            complement(23836..24624)
                     /locus_tag="PLM90_03145"
     CDS             complement(23836..24624)
                     /locus_tag="PLM90_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HMZ88394.1"
                     /translation="MKQKIYQVDAFAEKLFSGNPAAVCPLDQWPEDSLMQQIAMENNL
                     AETAFYVNNGDYYALRWFTPTVEVDLCGHATLAAAFVLWNYEGHSSDEIKFISQRSGE
                     LKVKRSGAMMTLNFPTDTLSEIGVSDDIKACFNISPLSAFKGKTDYMLIFNNESEITN
                     IIPHLDQINKLIARGVIITAPGDVCDFVSRFFAPQAGVPEDPVTGSAHTSLTPYWSKQ
                     LGKTELSAIQLSARKGYLTCKYLGDRVEISGKAVLYLVGEINVK"
     gene            complement(24681..25175)
                     /locus_tag="PLM90_03150"
     CDS             complement(24681..25175)
                     /locus_tag="PLM90_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ88395.1"
                     /translation="MADNLPLELTIDKASKTVTITRAFNASQDMVWDAFTRSEFLDQW
                     NAPKPFTARTKYMDFSVGGKRFYAMVSPDGQERWILQTYLVINPKTHFKYFNVFADAN
                     ENPDQFGSEWDISFSEQAGLTTVQISIFNESLERLERMLELGFKEGYAASMANLEALL
                     ENMQ"
     gene            complement(25177..25491)
                     /locus_tag="PLM90_03155"
     CDS             complement(25177..25491)
                     /locus_tag="PLM90_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ88396.1"
                     /translation="MRRDIFQAIADPTRRAIIALVALHAMTPNAIAENFDITRQSVSK
                     HLRILTECELLKQTPNGREIYYSLEVSKMKEVDLWLEQFRKLWEGQFAQLDQLLEQMK
                     KQ"
     gene            complement(25671..26429)
                     /locus_tag="PLM90_03160"
     CDS             complement(25671..26429)
                     /locus_tag="PLM90_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HMZ88397.1"
                     /translation="MNVKLRIWRQRNAQEQGKFVDYAVNDANPNMSFLEMIDVLNESL
                     VNKGEEPVAFDHDCREGICGMCSMYINGRAHGPQKLSTTCQLYMRNFSDGETITIEPF
                     RAKGFPVIRDLVVDRSAFDRIIAAGGFISASTGQAQDANAIPISKTNADLAMDAAACI
                     GCGACVAACKNASAMLFVSAKVSHLALLPQGDPERRKRALAMVAQMDAEGFGSCTNTY
                     ACEAECPKGISVANIARLNREYLNAAIVSEEMPK"
     gene            complement(26496..28427)
                     /locus_tag="PLM90_03165"
     CDS             complement(26496..28427)
                     /locus_tag="PLM90_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HMZ88398.1"
                     /translation="MLDSKIPAGPLSEKWTTYKNTVKLVNPSNKRKLEIIIVGTGLAG
                     ASAAATLGELGYKVKVFCFQDSPRRAHSIAAQGGINAAKNYKNDGDSTYRLFYDTIKG
                     GDFRAREGNVYRLAEVSANIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQ
                     TGQQLLIGAYQGLERQVGLGNVQEFSRHEMLDIVTIDGKARGIIARNLVTGELERHFG
                     HAVLLCTGGYGNVFYLSTNAMGSNVTAAWKAYKRGAFFGNPCYTQIHPTCIPVSGDYQ
                     SKLTLMSESLRNDGRVWVPKSREDAKAIREGRKKPTDIAEEDRDYYLERKYPSYGNLA
                     PRDISSRAAKEACDDQRGVGESGLAVYLDFSAAINRLGKHVIEERYGNLFDMYAHITA
                     ENPYEVPMRIYPAVHYTMGGLWVDYELMTTVPGLYALGECNFSDHGANRLGASALMQG
                     LADGYFVIPYTIGSYLAGEIFTKAIPTDHPAFEETEKHVRESLDKLLSIQGTKSVDHY
                     HKELGKIMWDYCGMARTAEGLKQAREMVKKLRAEFWQNVRVTGKANEFNQDLEKAMRV
                     ADFIELGELMIVDAYERNESCGGHFRTEFQEEGEAKRNDDEYAYVAAWEFRGVDNDPA
                     MHKEELKYETVKLATRSYK"
     gene            complement(28508..29212)
                     /locus_tag="PLM90_03170"
     CDS             complement(28508..29212)
                     /locus_tag="PLM90_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HMZ88399.1"
                     /translation="MNWFSTFLTSSIGRKVVMAVTGLFLISFLIVHCTINAMIFFNDG
                     GESFNKAAHFMGTNVIIRTMEIVLFLGIILHIVQGFMLSAKNRAARPTGYAVTNPGAN
                     SKWYSRSMTLLGTLILLFLVMHISHFWTHSRLGGIGGISPLDETMIGDKEVLNLYALM
                     ISTFQSPVVLIVYILGVISLCWHLVHGFQSAFQTLGVNHKKYTPVIKGIGIVFSILIC
                     ALFAAMPLSIYMGWIA"
BASE COUNT         8529 a         6467 c         5828 g         8524 t
ORIGIN      
        1 aacgcagggc agcaagtgaa acaacagaaa caggtttttc ggcaatatgt gtttccgtat
       61 atattttctt tgcagcagga atcagcattt tcatggcaag gcgaatatca tcacctgtca
      121 gtgccatttc attcgactct tcgtaagcta cacgcaattg gcgaataatt tcgcgttctc
      181 caactacaag ggaatcaagt gaggcactca cttcgcacat atgttctatt gcttcgacgc
      241 cctcaaataa ctgagaatgc atcacattac atgcttcgaa ttccgggttt actgttttaa
      301 agaagccggc agtaaactga ttgtctaccg gttgagtagt agtaaaaaaa tatgttactc
      361 gattacaagt attgagatac atcaactcat ccaatttcat ttcctgctta atggattgca
      421 gttgttgaga atagttggtt gtttgcttat cgaatggaac tatataattg ctgagttgcc
      481 ctagcgggac ctgtttatgt gtaacggtta gtactttata ctggttcacg gtttctcctc
      541 ttcttttcgc tgcttgaaat aactatgcaa aggtcttatg acgcagccgt gacattgtca
      601 ttgaattgtc atttgccacc ttttacaggg tgattttact caccgttttt gcacttaatc
      661 gcgtgtgaca gtgatgggac cggttgttac cgaattgctg agatttcctg ctctgtcgcg
      721 catccagacg gtaaaagtga ccgtttcgga atcgctgtcg gggtctacga ggatggtatt
      781 atgcaattgg acctctaaat cgccttctat tgttatttcg gagccttcag gagccagcca
      841 tggcacaaag aatgattcag tgagcggaat gcggttgtcg gtgatataaa gcgacataga
      901 atcaggttca ttatatccca aatcgccatc accgtcaata taatggatca gaaatacgat
      961 ggagtcctcg agtgccacca ctgtgccggg agaaacggac atcaattcaa tttcggggac
     1021 ttcagaaatt ggtggattta tttcttcatc tttcttacat gccgacagaa gcatgcaggc
     1081 gataatgata taagctgatg tccgcatggt cattcaattc tgaaggtgaa atagtaggcg
     1141 atataaattt gggaatcatc aggaagctcg gttacaaaat cgtgagcggt atctttcaga
     1201 tataatcgca tgaaatagag ctttcctgtt tcaaaatcat cggtatttaa ggtaaaatgg
     1261 tgccagtatg gggttgggat cccggggtca taatatgcag gcccgtagta tggatcgaaa
     1321 tcaggttcca gttcaaaagt atattctgta aatccggtat tcatgagcat gtcctgagaa
     1381 atgcggatat tgtattgttc gagccatgat ggcaattgat aaactccact ttcgtctgta
     1441 tcataaattc cgaaccaata ttccacattg ctatgttgcg ggaggataac aggttgaagg
     1501 aggtcagctc gatttgtgtc taacctcaac cctgtggtgt cattttcata gcagatatag
     1561 ccgtatgttg agggttgata atcaggtaat atgggtgaat tcccaaaaat atctggcgca
     1621 ccatttaata tccattgtgt aatcatttca cgttcatgcg gatagagcgt atcgtataat
     1681 ggcattcgtc cgagcacggc atcatcggtg gtaattcgtt catgcagcca tgaaagagcg
     1741 gtatcaccgg gtacaacacg ataagtaaat gtatcatcat catcattttt aattaccgga
     1801 gcatagacca gtgaattata ggcactttgc accgttctaa aatccggttc gaaagtaccg
     1861 tcgtggcaac ccggcactgc gcaggtggtg ctgaaaatga aggtatgcaa tcccaggaag
     1921 ctggctgaat caattaaaac cgggtcagtg cctccgttgc cataatcaac atcatcgtac
     1981 ggattatcag gcagatccgg ttcatcgcga atgatctcac ggcaggcgat caatgtaaat
     2041 gcacaaagca tcgacatgac ggcgatcttg gttatcggaa attgtttcat acgaatgctt
     2101 gttcaagtac tgttccgggt aaatatcctg aaggtatatt gtctttaaat ccgagaatat
     2161 gcgcaatcgt cggaacaaca tctattgttt gcccgacagg ttcttcttcc gaaccaacaa
     2221 caagcccttg atttacgata gaaggaggac cgacaacgag gcagaaaata tcgcgtgaat
     2281 tttcatcacc ggtatgatca taggcaaata atccattggc atccgggatg gtgtttgcga
     2341 ctgtatttct gccatgttca ggcacacaaa tcataacagt atcattcgct aaaatcggat
     2401 cactttgaat tttatcccat aaccatccta tgccataatc cgcgcgatgg agataagata
     2461 tatagctgct gaaattggta tggcagatat cgaggttggt agtatttatc accattaatt
     2521 caggttgaaa ttgctgcatt accttccatg aagcggcgat attagtcaga tcaccggtca
     2581 tctggtcgaa acccacacct gcaggtaaag gccattcaat aggaatctca ccatgcagca
     2641 atccgagcat aaagtcttca attgcctgct tgtcttcttc agaatttcta atgccgggta
     2701 catcaagtac tgacctgtcg aaattcgcat caaggaaact tcttactgca ttcattctgg
     2761 ttatctcatc aggatgcatg gtatttaagg tagagaaata atctacacca atatttccga
     2821 aagtagtgat cgggctcatg taatttgcgc cgtaattcgc gccgtataat ggatccttgc
     2881 tataattaag ataaacgtat ggtcctaagt caattgacat ccaccatgca ttgattgcac
     2941 ttttcagagg gtcattgtgt ttgcgataat attcaaataa tgttggcatg gccggatttg
     3001 ctgaaagatt gagactatca ggcgtataat tccctgtgat aacagcggta tgcccattaa
     3061 aatgtccggt aggaccgttt tcatatcgca tatgtttaaa cagggtacct tttgtagcga
     3121 gcggagatgc taatgccggc gcccattgat cataaaccag attataggta ggttgagcgc
     3181 ctgaaagcat attcggcatg atatttcctc cggggcccgc tgcctgattg agcagatatt
     3241 gctgactgac agattcctga tttctaattc caccgccaaa tacaactaac actacatgat
     3301 ttgccaatcg cataccggaa cttcttgaca aggttccggc aggcaggata tatggtgcag
     3361 caatcatgcc tgcagttgtc aataaggagt tgcgaatgaa ttttctacga ttcatgatca
     3421 gctgtatttt taataaaaca tatattcatt actaagcagg aaagcacgat acacatcaac
     3481 tacagtcata tcggcatcca attcaatcat atccgaaagc gcatattgtt catatgccgt
     3541 tggtttgcgt aaataaaaac gcagatagca gtcttcaaca aactgatcaa catcaccgcg
     3601 catatcagca tccgacggga tatttgccaa tacagcaggg tcttgcagca tcgcgtttaa
     3661 tatcaattca tttactagag ttttatcgcc aattgacaaa cggtaaatta caaggttatc
     3721 gaggatattg ccggggatac tcgaaaaata taaattactg tataatcctg aaatatattg
     3781 caatggggtt tttactctat ccttttcttc atttgattga taaacaggga cagtatctac
     3841 tccgtagata atattatcat aaactacttc ctgaattacg tcttttttac aggaattaga
     3901 caacagcact actcctagta tgcctaatgt tgagaataat ttagaagcct gcatattctg
     3961 ttgaagttaa gatgatcaat gccacaccat ttacattatt ttcaagcatc atttgctgcg
     4021 tgtaagtatc catttccaga ctattgggat cccttgcgag gtaggtatta aaagattcaa
     4081 ttaccaatcc ctggaagaat tcaggtgtgg aagttacgat atcaataaaa tcgccctttg
     4141 agctaccatc ctgaagaaat aatacatctg aaacaccatc gaccatattg atcccacttt
     4201 cttgttcatc atcagtagga taacgttgca taaggtttgt aaagcagctg accacaaaat
     4261 tcagactacc catatttacc tgatcgtaga aataattatt gatatatcta cgataaaatt
     4321 catcggcacc tatagtccca tttgcaagat cgatatgtgc agtgattaat ttaataagtc
     4381 tatcgttttc aaaaatatag tatcctgctt cgccttccat acccatatcg gtaaaaaggt
     4441 cgatcaggta atcccagtaa gctatctgac tggcaattgc aaaactgtcg acatctacca
     4501 gaaatttttg tccggtggtg aggtggatat tattgatatg ctcccaatcg gaaagaagtg
     4561 aatttaccag ttcttcccga gcttcaatac tgtattgttc tgattgaagc gtttccgcat
     4621 gttcacttag ttcatctgca gtcgccgtcc taccaaagag atcaatgtat aggctgttaa
     4681 tataattatt cagtgcaatt tcagacactc ctgtaacagg tggagcgata tttccatcga
     4741 tgatcaggtt ttcatggatt acctcatcct tagcgcagga aacagccagt aaataaacag
     4801 acgaaataag gataagccct tgtcgcatag tcgccatttt aagtgtgtta gggtgtgtgc
     4861 agacgttggg gcgactgtca ctataacgtc atgaaggtag taaatcgtat gtgtacacgc
     4921 aaaaaaaaac gaccccgatt tcgcggggcc gtctcctttt catactcgaa ctgttttttc
     4981 cggggctatg gatttatgtc tttcttttct tggaatgcaa taacgcaatt gctttgtgcg
     5041 atattgtatg agacaaaagt agatcggttt ggtacactgt acaagaggga gtattaacga
     5101 ggttgtacac attgtacaat atagtacacg gtggtgttag agagtacgtt ttattacctt
     5161 gaaggcaagg taatcctttg attagaattt ataagcgatg ccaaatccga tgcttccaag
     5221 agagagcgaa atgctttgtt gggtaaatac cgcatcagta gttttatcct ttgtttgata
     5281 gccgctaaat cctagtgatc cgcatgctaa ttccaagcgc cagtggtcat tgaaaattat
     5341 agaagcaccc ggtccaatac cgagggaata atttatcaag ttatcggttt gctcattaat
     5401 agatccaacc ccgttatcgt cgaatactat ggattcatcc gtattctttc caaagcccac
     5461 ataaacgcct gctccaagcc agaggtcaac atactgcgat tgaaagggat aatatcgggc
     5521 aacgggacct ccacctacac tagagcgtgt aaaatgcata tccaatgcag gatcatcagg
     5581 atggtaaata gttgtaatag aattgttgaa tgctgctctc aaccctatca tccatttgtc
     5641 gccaataaaa tatccaactg cagggcctgc atatattgtt gacgatgatg ggcctttatc
     5701 atcatatcct gttccggatg agtttgaagt agtgtttgat agcaacaagc ttccacccaa
     5761 taaccaatca ccttcggtaa attgagcttg agcgaatatt gggatagata ttgccagaaa
     5821 agctaagtgg atttttttca tcataccacc aaatataact attcaggaat aatttgggtg
     5881 gtataagaaa cgccgtatgc gcaccagata cctaatcccc ctacaatatt agtttgtgcg
     5941 atagtagcag aactaaatgg accgtcggtg ccactatcaa attcaagggt tctccagaaa
     6001 tcgtatgtag ggaaatcgat atgacaccat ttcagggtaa cggtatcgcc tttccaaaaa
     6061 tagccatagg ttcccatgct gtaatcttct gtcgggtcgt aacctctgtc gagagtaaaa
     6121 tcaaatgttt gaccattgat caggttgtca tcaaaaacac ttgagtatcc ggaataaaaa
     6181 ggatcgctgt ttcttctggt aaaataccga taaaaatcac cttgcggttc cggttcagaa
     6241 agtgttacat ataagcgcat caaggagtcg ttttccggat cggggtgcgg ttcgaaatac
     6301 atcgtgtcta aagggactgc aggcaacaaa cgcgttgatg aagtaatagt ttgtccgtta
     6361 gcctcgatat gcagatcata tgtttttcct tcttcaccca caaatgatgg tgaacctgta
     6421 aaaatatcag cgaccgtata caaacaaacg tcaatagcaa gttcaccggc tgtatccagc
     6481 tcaacgccga gatattcagc aacaagaggt aaaatctcat ccggtaattc acttaaacaa
     6541 tattcaacca gttcaacagt attaacacca tcactcacgg ttacaacagc atcatgtacg
     6601 tagtaatttt ccagttcgtt aaaatttact tcgccataaa aaggtatcgt tgaagtcaat
     6661 gaaattattg gcggcacacc attttcaata gagccttcta caactatttg cggctcagga
     6721 tcaggtatat cgagtgtaat atcagttgta catgaataaa aagacatcaa taatatcccg
     6781 aaagaaatta cgccgattat ttttgactta ttggttcgaa acaacatgtt agtaaaaagt
     6841 ttaagggatt tctattatat atgttgctga gcaaatttaa agttccatgt tactgatgga
     6901 attatgggga aaatagacac ctgtttcgcc tgaacatcca gatatccgta tgcaaaatcc
     6961 ccttcgtaag tggtgtaaat gaaatacgga tttttacgcg aatacacatt ataaatactg
     7021 aaattccagc tactttcata tttcctttcc ggtttgctat gtaatgtagc agatacatcc
     7081 agtcggtgat atgcaggcag tcgataacca tttcgcggac cataatcagt ttgcacctga
     7141 ccgccaatta cgtaaaaact ttccactatg gttgtagcct gtcctgtggc ataaacaaag
     7201 gtgcttgaaa agtcccatct atcgctgaga ttgtaaacgg caacaacttc gagatcatgt
     7261 acgcgatcat atttggttgg gaaccattcc gagcctgtgc tgaggtcttc aaatttacgt
     7321 tcggttttag agagtgtgta accaatccaa ccattaaatt ttcctttagc ttttttcagg
     7381 aatatttctg cgccataaga tctacctacg ccaaacacga aattatcttc tatatcaagt
     7441 cccaattcag gaacatatcc atctctgaat tcgatttgat tgcgcaagtc tttgtagtag
     7501 acttctatac ttgtttcata ggcattatcc ttaaaattcc ggaaatagcc tagggcatat
     7561 tgaattccaa tttgaggttt tacgatgtca gagcttggta tccacagatc agtaggtaaa
     7621 gtacttgttg aactgcttac caaatgaatg tattgattat tgtatgtaat acctgctttc
     7681 aacgacgact cgggatcgat ggtaaagcgc atattaaacc gaggctccag tccggagtag
     7741 tgcgctatca tttcaccggg agcgtaataa atggtatcaa taactgttgc tccattttca
     7801 tcatatacta tatcattata agggcccacc tgtgcaaaat agctgcctct taaaccgata
     7861 tttattttca gtctatcagt aacatcaaat tcatcttgaa taaatatgcc ggtttcatgt
     7921 gcatatttat tacttaacgt gtcggtagag aagtcgacat catcaaaact tccttgtgct
     7981 gaatatggtg taaatgtatg atagacataa tttgctccaa attttatgtg gtgatttgaa
     8041 ccggggtagt aatcgaaatc caccttccca ttccaatcct gaatgcctga atagaggttg
     8101 aaattaaaat cctgaaaatc gctttgcacg gagaaattaa aactgttgta gatagcgcta
     8161 acattcataa acaatttatt attgaacagg tggttccatc gcacagtacc tgttgcattt
     8221 ccccatggca cagaaacgtt taatgaccca tcgggggcgc tgtagttaaa aacatcttgg
     8281 ccgaagtagc cgctggcaaa aattctattt ttatcgtcga aggtatagtt cacttttgta
     8341 ttcagatcgt agaaataata gccattccct tcaaaatccg ttccatttaa aaaaggagtt
     8401 atcaatgcat cgatatatgt tcggcgaccg ctaacaataa aagagctttt atcttttacg
     8461 attggtcctt gtatcgttaa tcgtgaagca ataagtccta tgccaccctc agcctggtag
     8521 tattgattat taccttcctt catttgaaca tcgagcacac tgctgaggcg accaccatat
     8581 tgtgaaggca taccaccttt taccaatgta acgtttttta tggcatcgga attaaacacg
     8641 ctaaagaaac caaataaatg tccggtattg tataccacag cttcatccaa taaaatcagg
     8701 ttttgatcgg gacctccgcc acgcacataa aatccggtat tgccctctcc tgccccacca
     8761 acgcctggaa ggagttgaat tgttttaagg atatctactt ctccaaacaa cacgggaatt
     8821 gttttgatct gttcgatatt caattcaacc ttccccatat cagtgctttg gatattcaca
     8881 tcaccgcgtt ctgccttcac aactacttcc tccaataatt taccttgcga taattgcaca
     8941 tttaacctga catcattatg caatgacacc ttttctatct gattatcgta accaacataa
     9001 cttattacta cattgtaatc gccctccggt aaggtcatag agaaaaatcc atatccattt
     9061 gtagaagctc ctgtgccggg gtcattttgc agatacacgt ttgcaccaat catatcctca
     9121 ccggtacctt catccttcac atatccgcta agagtatatt ttccttgtgc aaacaacatg
     9181 ctgcatgcac acataagaag cccgaaaagt cctgttttca tctgatactg ccaataacgc
     9241 cacaaagtaa cgttcagttt gctgtacggc gtaatttttt tctacgaacg cggaaaaaag
     9301 gtcgacgaat gtcattaaat ctcttgttcg atcattgaat gtagatcaac ttcgattcgg
     9361 agtagatgtc gccggttagt cgaatggtat ataatccggt agcgagtcta tctaagtctt
     9421 caattctgat tccgctcatt tttcccgcat cgatgagcaa gtcagtgctc atgatcattt
     9481 tccctgccat gtcgaacata tcgaatttag cctgatcagc ttcgaatcct tggatataaa
     9541 cgacaagaac atcgctgaca ggattaggat aaacatggag tatatcatca ttcaaggtaa
     9601 tacctttcag tgccatataa gcatgcccga attgaggaat tccatatcct atctggttat
     9661 ttggcgtttc ggcattggta gcggtttgtt gaatgatatt gatgacatcc atatttgaaa
     9721 gatcgggaaa tgcggacatg aagctggcgc atgcacctgc aatcagagga ctggaatagg
     9781 atgttccatt gccataaccg acaatgcctt cagccgacat aacggcagtt ccggcacctt
     9841 gtccaaccac gttcggcttg atacgaccat caaccgttgg gccgactgag ctaaatccgg
     9901 cataatttcc attcaaatca actgcaccaa tggtaaacac gctatcacca tcagcggggg
     9961 cgccgataaa atgccagggt tcatctccac tatttcctgc actattgaca actaaaattc
    10021 ctgctgcagc agccatatct gctgcaattg ttgcaacagt agtgttgcca tccatatcga
    10081 gataggtatg gtcttcggta gtgtcatcga aagtagtgta acccaacgag gaattaatga
    10141 catcagcacc aatgatatct gcatattctg ctccttcgag ccaataatct tcttcaacaa
    10201 catgctcact gtcagtcacc tccgtgcgaa tgaggtaata atcggcatcg ggagcagagc
    10261 ctacatacac acctggttca tcaacagcca taatacttaa cacattactg ccgtgtgtag
    10321 aactgaaata cacaaaatcg gtatcgtcgg gaaaattata ggtgcctttt atctgttctt
    10381 tggcgcgcac actttcaaat ccggcacctg tattcacgcc ttcaaagcca ccatctaaaa
    10441 cagcaatggt aataccctgt ccgcggaaac ccagcgaatg caaaaaatcg acttcaatca
    10501 tatgattttg attctgcgct ccgccataaa caaaatcagt gatatcggcg gtgcgataaa
    10561 atgcctgatc ttgaggtacg ctaacatcat ctataaggtg tcgtttaata accggagtga
    10621 tttgctttac aaacgacaaa gcggagattt ccgcaatcat ttcaggacct gtaacagtga
    10681 cacttactgc attgagccat ctgctttctg tgcgtactaa agcaccggtt tgtttaattg
    10741 cgctaatata ttgcggatcg acgggaagat cagattttcg aattggaata agcattcgct
    10801 cccttctatc gatagcttgt tcagataaaa atgcagatgg gttatcaata gtataaggtg
    10861 tggcattttt atcgctaaaa tacacccata tattttgcga ttgagcaaat gatacctcgt
    10921 gcatcattat actgaaaacg aatatgccta taattcgaaa aaaatacatg caaatcttta
    10981 ttctataaac accttgacac tttgtttata ccgccccgac ctgaccgtca gaaaataaat
    11041 acctgatgca taaagatgct tattacccat ttcaacagtt gttggcattt ctgcattgag
    11101 gtacacattt tcctgatgca tcagtgcgcc ctgcaagttg taaacggcaa aagtagcttc
    11161 acggtttgtt aatgattgca atatcacggt aaaaatatca ccatcgagca atggtattat
    11221 ggctatagag gataaagtgg tgtcaatggt ggtatcaact gttgtatcaa caacggtggt
    11281 atcaatttcg gggaataatg aatcaagcaa agcatatgct tttccaaaat taggaatgcc
    11341 ataacccatt gaatcgtttg gtgtatcgta caagtgggca ctttgttcca cagcatgcaa
    11401 tatttgcata tttgtagctt cagggtatgc ctgccataaa gttgcacagg cacctgcgag
    11461 taatggtgat gaaaatgatg tgccactgag gtaattgata tacccattcg gatccaacac
    11521 agcagatccg agtgccaatg ctacaacatt tggctttagt cgcccatcaa atgtaggacc
    11581 atatccacta aacccgacat ggataccgga agtgtcaact ccaccaacac tcagaacgct
    11641 atcgccatca gatggcatcg aaacataatg ccatgcacct gctccttcgt tgccggcgct
    11701 gttgcaaacc aaaattcctt ttgatgctgc catatcagcg gccattgtaa caacagtggt
    11761 attgccatcg aggtcttcgt aagtatgatt ttgtgttggg tcgtcgaata cggtatatcc
    11821 taatgatgaa ttgataatat ccgcaccaac actatcagca cgttcggcag cagcaagcca
    11881 gtgatcttct tcaacaacgt attcactgtc ggcaatttct gtgcgataga ggtataagtc
    11941 ggcatttggt gcagatccgc tgaaaagatc agtcacttca ccggcgatca cactcatcac
    12001 ccagcttcca tgatatcccg aataaatatt gaacacctga tcattgttat ccgggaaatt
    12061 atgataccct aagatttgat ctttatccca aaaactttga aacaaggtag cagtatccac
    12121 gccaaaaaaa ccggcatcaa gaacggcaat caccattgaa tcacctgaaa gtccgatatc
    12181 gaataaggta tcgagattaa tttgttcaat ctgattactt gcggctccga gtcccccggt
    12241 aatttcagta ttatctgcct tgcgataaaa tgtttgatca ttaagtgggg tgccttgttc
    12301 attgaggatt tgatatctac ctacccttga aattttattt acgaatggca attcactcac
    12361 ctgcagcatt tgatcatcgc tcatgattac acttactgca ttcaaccatt tagatcgcac
    12421 atatacctgg acaccgatat tttcgagtga ttgaatataa tattcactta caggcaggtc
    12481 gctttcagaa ataggaatat gttgtttttc gcggcgattt aaagatgcct ttgatagaaa
    12541 ttgctccggt gattgtttag aaaatgtatt aaattgttta tcggtaaagt atacccaata
    12601 acgatctgta tgttgttggg cgaaagaggt aatcgacaaa cagcaaataa ggcaaataag
    12661 gagaaatcgt tgcatgaaaa attatagttg ataatcagtt aatcgcatat aaacactgaa
    12721 tccgcattgc acgcgttgtt cccatggaca atccggacag gtaatatctt gcgtataata
    12781 atgtttgaaa gttttttcca ccataccgac atccaaggca taaatctctt ttgcataatc
    12841 aaaccagata aggttagaat cgccgaattg ttctacggta attgtgtttt cgaaatcgaa
    12901 atccggaatg gaataatttt catttacatt tgaataaata taatcccatc cttcataata
    12961 aatcaggcga ttgcattctt catcataagg caggtcatct aatcctccga gatatacatt
    13021 tccatcccat tgaacatcat ttaatggagg agaaacaagt tttacaaaac gcaaattatt
    13081 ctcatttctg atcattttgc catccacttg catcacactc catgtattcg tcaattgcca
    13141 tgcatcgaca tctgtttttc tgcgatatct gtcaatgaca taattaagat cgccggttaa
    13201 atcataaaac gtatcaatca attcttctcg gacgaaccag agagtggtgt cattaccggt
    13261 agtttcgtga tagataatag agtcgacagc atactcccaa tagtttccta ttttgagcgg
    13321 gctgaatgcg taaggtgttt caggagcaat cggttcgggc aaaggtttat cacaacccat
    13381 tgaggcagca aaaatgaatg ctgtgagaat caaacccgtt atggatcgag aaaacattaa
    13441 tcaaagatac aagaaagctt caacaggcgg agaatcaagg catttggtgc aattttcgaa
    13501 caaccattag tagattcaat tgtaccaata aaaagtaaca atcaattaca tttaacggcg
    13561 gcaatatttg gcaaggttgc attactactg ctgaaaggca ctcacaatat gcccaccacc
    13621 aatcacatcg ttgccctcat aaaatacagc gctttggccc ggtgtaattg cgctcacatt
    13681 ggagtcgaac gctatgcgta gcagatctcc cggttcgcta aacagcgtac tttcggcacc
    13741 agcattttta tagcgaattt tcgtcaccgc acgcatcggc tccttgatat tttcatattt
    13801 gagcatattg aggtgatgca caaacatgcc atttttattg agttcttctt ccagaccaag
    13861 cacaacaaca ttttcgtgtg gaatgatctc cgtcacaaaa tatggttcac ctaaggcaat
    13921 tccgaggcct tttcgctgac cgatagtata aaatggatag ccttgatgag tgcctaacac
    13981 tttgccgtgt ttatcgacaa attttccgcc ttttaccttt tcctccaatc catccacgcg
    14041 acgtttgagg aatccgcggt aatcattatc gggaacaaaa cagatctcat agctctcggc
    14101 cttgttggcg agttcggtgt aacccatatc tcttgccatt tggcgtattt cagtcttatg
    14161 aagttgacca agcggaaatc tggtgcgaca caggcaatcc tgactcaatc cccatagcac
    14221 atagctttgg tccttatttt catctttgcc cttactgata aagtagcgat tgttgtccgg
    14281 attttggtgc acgctggcgt agtgaccggt agcaataaat tcacaaccta attggtcggc
    14341 acgtttgagc agtgcttccc atttgatatg ggtattacag agcacacagg ggtttggtgt
    14401 tcttccggcg agatattcat ctacgaaatt atcgataaca tagtcgccaa attcttcacg
    14461 tatatcaact atgaagtggt gaaacccctt gtcgacggct accatacggg catcgttgat
    14521 agaatccagg ctgcagcacc cggtttcttt ttttgagcct ccgctggatg cgtaatccca
    14581 tgttttcatg gtaataccca ccacctcata cccttgctca tggagcatga tagcggtgac
    14641 ggtgctatcc agtccgcccg acattgccac taatattttt ccgtgtttcg acatacgcct
    14701 gtggcaaagt tacgtcagta aaggggaatt agttcccgaa gctagttgca caatatcggc
    14761 aggtacgtct gtttagtagc ctcacaagtc gtcgataaat cggtcaatgt ccctgcgacc
    14821 ctgcttagtt gcatagggat ggaggtgcaa agcgaatagt tcgaggtaat gtttgaatag
    14881 tgttcttttt ccggcatcat tgaaccgtag tcgcccctcc gacaaccaga atcgcgacat
    14941 gaggctaatg gaagatacga gaaaatcgag tgtgcggtcg ttgagggagg catccatata
    15001 accgttttgc atgagcagct tcaagttttt tcggatggtc ttgtatcgtt tttcttgatt
    15061 ggggatatac acctctttta cttttggatt atgctccatg aggtatacaa aactcacaaa
    15121 taagcatcgg tatttatatt ggttggatga ggtctcggag tacatttgta ggaaggtgct
    15181 cattgagagg tcatccccgg cttcaaggat ggtattgttg gcatctgcca gttcagttac
    15241 aatagcggca actatatcgt ctttggtcgg aaagtaataa gtgatgtttc caacacggat
    15301 gtcaagttcc tttgctatct ctcgcattcc cacatattct atcccctgct cattgaacat
    15361 gcgcaaggcc tgattgaaaa tattttcttt agtagtattt ttggacattg tcctaatttt
    15421 tatatctttg gacattgtcc aaaattatac actttatgaa gacttacaaa ctaccttaca
    15481 caattaggaa caatttcggt gagatattgc atttccagtc gatacgaacc gagggatcca
    15541 aaacatttat tgatgtgacc aatgaagtac agcccggatg cggaccggtg atgcatgtgc
    15601 atttcaaaca ggacgagtat ctgagagttg tagaaggtcg catgggatac cagatcgccg
    15661 gaggggcgga acagtttgct ggtccgggag agtcggccac gtttcatgca gggcagatgc
    15721 atcgtttttg gaatgcgggt gatgatgtgc tgaaatgcga agggtggata tcaccaccga
    15781 acaacatcat ttattatttg gagaatatat atcggttgca gaatgaggga ggtggggagc
    15841 cggggggttt tgaggctgcc tacctcattc ggaaataccg ttcggagttt gatgtagctg
    15901 caatcccggg atttgtaaag aatgtgatat ttccaatagt agtgttcttt gggaaattgg
    15961 caggcaagta taggaaattt gcagatgcgc cgcccgcggt gaggtgacgt tccggttcgg
    16021 gaaagaggca tgcttttagt aacgcgagta agcgattaga agcggttatt cgggtttatg
    16081 ggttggaaat agggcatctc aaaaaaacac acctaacagg tcttagacct gttaggttta
    16141 agatctgtta ggtttaaaat ctggcaggtt ttagatgtgt taggtctcaa atcccctcac
    16201 accaacaacc ccctcaccat cccctgcccc tcataaatca tccccgtata aatttgaact
    16261 agatccgccc ccctgtcgaa ttttgattgc atcgacgctt tatccgtaac ccctcctacc
    16321 cctatcaatg gaacatccgt tcctaaagcc cttcttagaa accaaagtac gtcatttgac
    16381 cttgacaata ccggttttcc actcaaccct ccggcaccta tagactccac agcagcctgg
    16441 ggtgttttca gcatactgcg atccaaggtt gtattagtcg ctatgatacc gctcaacccc
    16501 gtttctcgtg tgatctcaac aatatcattt aactgcgcat cattcaggtc cggcgcgatc
    16561 ttcaacaaca atggctttgg ccttttctta ccctgattta tttcttgcag actagagatt
    16621 atacgcttaa gcggttcttt ttcttgtaag gcccgcagac cgggcgtgtt cggacttgat
    16681 acattgatca caaaataatc tacatggtcg aatagcgcgt tgaagcaaat gacataatcc
    16741 ttccatgcat cctcattttc tgtcactttg ttcttgccaa tattaccgcc aatcaccaca
    16801 cgatatttct ctctggcatg cgctaccgat aattttccaa gattcctcac catcatttca
    16861 acaccttcat tattaaatcc catgcgatta ataagcgctt catctttagg aagcctaaac
    16921 aaccgtggtt tatcgttacc cggttgtgcc ttcggcgtga ccgttcctac ttcaacatgt
    16981 ccaaacccta acctcgccat cagaggaata aaggccgcat tcttatcaaa tcccgctgcc
    17041 agcccaatag aattcttaaa cctgattccg aaaacttctt tctctaattc cggccgctca
    17101 atcacataat gcttatcgtg ccaccatcca tatcccggaa tccacaaagc agccttgtag
    17161 agccctaatg ccaaatgatg agccgtttca ggctgaaatc gaaaaaacag cggttttaat
    17221 aaggtcttat acatccaagt acaaagtaaa tgagaaaagc cgataagctt aaccctcaaa
    17281 ccctcaaacc agaagtaaaa aaacaaacac acctcctcca tccactttcc tcccttcctc
    17341 ccttcctcct tcctcctttc tccttcctcc ttcctccttt ctcctctctc ctcctcccat
    17401 gttcttatct ttgcggcgaa ctcattttca agggcattgt tataagacta tggcacagat
    17461 cagcatcaat atcaaagaag gaacattaaa aagggaagaa attattgttg gtatagattt
    17521 aggcaccacc aactcactta tcgccataat agataaagaa acaggcaccc cttctgcatt
    17581 acgcgatgcc gataaattcg ccatagttcc ctctatcatt cattttgctg atgatggcac
    17641 tattactgtt ggtagaagcg cccgcgaaca tctgattact gatcccgagc ataccatttt
    17701 ttctgtcaaa cgtctgatgg gaaaatcata taaggacata gccgcatatc aacatacttt
    17761 cggttataaa ataattgatg atgacgccga acgtttggtg aaaattaagg ttcgcgaaaa
    17821 atattattcc cctatcgaac tttctgctct gatactcaaa gaattaaaat tacgtgccga
    17881 gcgtttactt caatgtgcaa taacaaaagc tgtcatcacc gttcccgcat atttcaatga
    17941 cgctcagcga caagctactc gagatgccgg aaaattggcg ggtctggatg tattgcgtat
    18001 tatcaatgaa cctaccgctg caagtctcgc atatggcttg ggaaatcaag ataaatcgtc
    18061 acataccata gccgtctatg atcttggagg cggcaccttt gatatctcca ttctacgcat
    18121 tacaagctcg gaaaactcag gcgatttttt tgaagtatta agcacaaatg gcgatacttt
    18181 tttagggggc gatgacattg atcgtgccat catggatcat tggctgcaaa ctcaacatgt
    18241 tgatggcaac ttattatcag caaccattcg tcaggatttg cgactcaccg cagaaactgc
    18301 aaaaaaacac cttggttctc atggtgaatt taccggcaac gttgataaaa tacaatgcga
    18361 tctaaccatt gcagaacttg aaacattaat tacaccactg ctacaaaaaa caattcagtg
    18421 ttgcgaacgc gcattgtttg atgcaaatct caatgtgaac gatattgaca ctgttgttat
    18481 ggttggtgga agcacgcgca cacctttcat caagaaaagc gtttcggcat ttttcaataa
    18541 acctgttcac gatgaagttg accctgacga ggttgttgcc ctcggcgccg ctgttcaggc
    18601 cgatattctt gcaggcaacc aaaaagacat gttgctgctg gatattactc cactcagctt
    18661 aggcattgaa acgatggggg gactcatgga cgtcatcata ccacgcaata caaaaattcc
    18721 aaccgctgca ggaaggcaat ataccactag tctcgacggt caggccaatc ttaaaatatc
    18781 tgtattccaa ggtgaacgag atctagtgcg cgacaataga aaattggctg aatttatttt
    18841 atccaatatt cctgctatgc cggccggatt gccgaaaatc gaaattgttt ttcaaataaa
    18901 tgcggacggc attcttcaag tgcgtgcaaa agaattgcgt agtggtgttg aacaaaaaat
    18961 tgaagttacg cctcaatacg gattaacaga agaagaaatg gccgccatgc tgctcgactc
    19021 tattaaaaac gccaaaacag atatggctga aagagcaatt gttgaagcca gagttgaagc
    19081 acagaatatg atagacacgt gtgagcgatt tattgagaaa aacaatcagt atttaaatga
    19141 acaggaaata gcaggcacca aatcactaat cgacaatcta cgtaatacac ttaattccga
    19201 tgacaaggat gtcatccacc agcaaattga aattctaaat gagtttacgc gaccatttgc
    19261 agagcgactc atggatatgg ctatcagcca ggcaatgaaa ggaaagacga tttaagttgg
    19321 aaataaattt tccgtgagat tataatacaa aaccggaaaa caatcaacgg gccatcattt
    19381 tttcgacata tttaccgata atgtcaaatt cgatattcac tgaatcactt acggatattt
    19441 ggctcaaatt ggtatgttcc catgtataag gaattattgc cacctgaaac gttccctcag
    19501 aaacattaaa aactgtcagg ctgattccat taatggatat acttcctttt tgaacgatgt
    19561 atttatcaaa ttgcgcatcg aatcgaaatg tgagcaacca gcttcctcct tgttcgtcaa
    19621 cctgaattaa ttgcgcggtg gtatcaacat gaccttgtac gatatggcca tccaggcgac
    19681 cgtctatttt taaacagcgc tcgagattaa ccgtatctcc gatttttaaa ttacccaggt
    19741 tggagcgaat caatgtttca tcgattgcag taactgtatg tgtatcgccg gcttgatgaa
    19801 caaccgtcaa acacacacca ttatgactta cactttgatc aactttcaaa tcacgactga
    19861 tttcggattg gacagtaata tgcaaatttc ctgcatcttt ttgcaatccc gaaacacttc
    19921 ccaatgattc aataatcccg gtgaacatta tacaaagata ggaattgcac tttttgaaac
    19981 acaatattca taacaaggga agtaacactt caccctaact gttattccac cttgctctcg
    20041 cgatacaggt gaatataacc ttccttaggc tgcgggaaag gtctgacgcc atcgacagtg
    20101 atttcatata ctgtacatgt atagaaatac acaccttcag aagcattctt tcctgtattc
    20161 gtatcgatac catcccaatt cagcataggg tcatttgtag taaatactac attcccccag
    20221 cgatcgaaga ttttcatatc cacatgatca atgaatctgt atggaagaat tggtgtgaag
    20281 atatcattaa acccatcacc attcggagtg aaggtgttag gcaaattata atcactgcaa
    20341 ttatcaacgc aaacaatatt actaaaagca ctattattat caaacgagtc aacagcaact
    20401 acagcataac aaccggcaag tgaaagcaat ccatcatgtg tatacgtagt atcaaatgca
    20461 tcggcattct gatctaataa tgtcaacgga tcaccttgtt gcgaagcgta ataaatcaga
    20521 tatttcacca catcgtcggc acatgaatga tttggatttg tccaggtcaa atcatttttc
    20581 aaatccgatt catcaacaac tatcgaccca tcctcttcac aaatattata gactgtcaat
    20641 atcggtggac aaggaggctc attgtctatc ggaacgccac attgtatttg cgataaatta
    20701 attaatgtat ccggaagcga ctcaatggta tatttgccat aggcttttac atagtagcaa
    20761 tactcagaac cattcgccaa actatcatcg atataacttg tctctgtagt acttgttaac
    20821 aattcgaaat caccggagcc cgcagtggtt tccttgtaaa catcataata aaaattcaac
    20881 caaggcacat taaaattcca agaaagattc agcttattat cgccgggtga tgctgttaaa
    20941 tacaccgatg aggcaggatc agtaacacct aataattcaa aatttccgag atcggtgaaa
    21001 taaaatttca atcgataaca ataagcagtt gctgcggtat tcaacatagt atcgatatac
    21061 gaagtatcgg ttatttcaaa aaactcacct gctgtaatcg ttgtcactaa tgtctcggta
    21121 cctgttggtg taaagccttc ataacgcacc aattcatatt tgtacggtcc tgtaaaaatg
    21181 gtagtgtcta aatcaggaga aatcggatta taccaaccca catacatact tccattagtt
    21241 gttgatgtgg tgcgaacact ggcatgatta ataatcggaa tatcgcgttt caattcggca
    21301 catacagcat cagatggcgc tccgggtact tgattgtaaa caaaatcggg tgcgatatca
    21361 ctgcgttcac cgaactcggc aactatgcga taagcatatt cctgtccata gtcaacagcc
    21421 aaatcaacat aggaataggc atcttcaaca aatgctaaag agtcgtatcc catccctgaa
    21481 agtccgcgtt gacatttatc aaacattgtt gaatcacaac caattttccg ccaaacacta
    21541 aatccaagaa aattatcggc acctgaacat atatatggat cctgccaggt cacagttatc
    21601 tcatttgctg tcggcactgc atcaagaata acaggtggtg gagctactac tgttatcaac
    21661 catgtttcca ggtcgacaag cggaatttct tcaaacccga tttgaaaatc atcttctgct
    21721 ttaaatacaa cttcatacac ttcctttctt acatggttgc agatagtttg ccatgtaaat
    21781 gttccgttca ccgaaccttc atttggtgtg ctgaaaaatt ctgcagggct ttcatcaata
    21841 tataatggtc cacctgtagc agtcaaggta attgtttgca aaacatcagg atcagttgct
    21901 gtaaccagaa tatttaattc ctctcctgca tacacacagg tgtcgttaag ctctgcaatt
    21961 tcaggcgcat gattgcttgt tttatttaca aaaatttgca tatcgcgcag gatcgtgccc
    22021 atcttgatac cattgcgata ttctgtaatg agaatggcga tattatatat cccttcggta
    22081 cacgacggat cctcccaggt gacatctcca ttgaacgtat tgatttgaaa aacatccgta
    22141 tcagtgcaaa aggtgcgatc atcaggatcg agataaccag gaactacaat acnnnnnnnn
    22201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaggttgt gatggtgaat tcgtaagtaa
    22321 gatcttcaac atgggtataa gtaatctccc ccgccctgtt atgggtggcc caggctttag
    22381 gggccgaaac caccaccagc agcatgaaaa tcaaagcctt acaaacgcgt ttcttcaaca
    22441 tcagttgcta atttacgacg aatggaggtg gattattgca atgttccggt aatatttatc
    22501 cgttaacccc acaattttta ttatcaaaaa tcatgcaaag cttttctgat cagacccgtt
    22561 caaaaattga tgcctatgca gtgactttgc aagtataact aattgatttt ctgattgtta
    22621 aaatatatac atatcaacta attatgccta ttccggagat caagcaaatg aaaaatatca
    22681 atcgtatttt tgaactggcg atagtcttat ttataagcct cgcttatcat tctgtctcac
    22741 aagggcaatt ttgttcagta gttaccaatc ggccatcgac ccatttattg gtgatatcgc
    22801 aaacgttgcg gaatccggtt tctgcacagt ttataacctt ttgcgacata actaatttcc
    22861 tatttggatc aggtattggt gaatataatg tatcagcttc cggatggtat acaagtatgt
    22921 tatcaatatc atctgttatc actaagtttc cttgctataa tgcaatacca ttagaagtta
    22981 cactagtgat gaaaacatca cgaacaccat caaaatcgta gtcagcaaca ttgagatgat
    23041 aaaaaacacc cggccccctt aagccactta gagatgatat caacttccac gtagaatctt
    23101 gtaatgcgta acgcgagatc agcatggttg aatctgtatg acgtgtaatt gcgaaagcat
    23161 acagtttatt ctcctgcgaa cgatatgata caaaagagat aaacggttcc catctgtcaa
    23221 atttcatagt atcctgaagc atcgcttttt tcaataattc ttcaactgaa ataccctggt
    23281 aatctgtttg ctgcacctcg gttgaagtct cctctgtcaa atgttttcag gcggaaaaac
    23341 acatgaccac caaactgatc agaaataatt tcatttttta tgtaatcagg ttcgaaaaag
    23401 gatcaagcag caaatccatc tttaatagcg tgctgtaaag tatccatatt gatatctttc
    23461 agtgctttaa acttgatgca atatccggtg atagtcgcct ttccaattgc ttgagcatag
    23521 gtatctgcca gatatttttt atcggccatc cccatgatat acactgagat tccactggta
    23581 ttcgcactca gtcccacctg ataaaaatct tttgcagaac catctgcata tttgagttga
    23641 aggagtccat acccaatatt cggattggaa actactttgc ctgcttcatt cttgccatcg
    23701 aggaaccaca acttagcttt tggttttatg tacagtatcg atttgtgtaa tgcaagcaga
    23761 tcattccgtt ttgcttccgg ctgactgctg atatattgct gaatttgtgt ttctatcgac
    23821 atacttgatc tattattatt tgacattgat ttctccaact aaatataata ccgcctttcc
    23881 actgatctct accctgtcgc cgagatactt acaagttaaa tatcctttgc gcgctgatag
    23941 ttgtatcgcc gacaattccg ttttccctaa ttgcttcgac caataaggtg ttaatgatgt
    24001 atgcgctgag cctgtaaccg gatcttcagg aacacctgcc tgcggagcaa agaatctgga
    24061 aacaaaatcg caaacatcac cgggtgctgt gatgatcaca cctctagcga tgagcttgtt
    24121 tatctgatca agatgtggaa taatattcgt gatctcgctt tcattgttga agatcaacat
    24181 ataatcggtt ttacctttaa atgcagacaa gggactgata ttgaaacagg ctttgatatc
    24241 atccgaaacc ccaatttctg aaagtgtatc tgttggaaaa ttcaatgtca tcattgcacc
    24301 tgatctcttt accttgagtt caccacttct ttgggaaatg aatttaatct catctgatga
    24361 atgtccttca taattccaca aaacaaaagc agcagccaat gttgcatgtc cgcatagatc
    24421 cacttcaaca gtcggcgtga accagcgcaa agcatagtaa tcgccattat ttacataaaa
    24481 ggcagtttca gccagattat tctccattgc gatctgctgc ataagcgaat cttccggcca
    24541 ttgatccagc ggacaaactg ctgcaggatt tccggaaaaa agcttttctg caaatgcatc
    24601 tacctgatat attttctgtt tcatgatctt accaaatatg tgtggtaaca aaactaccaa
    24661 tacaaatcag atcattgaaa ttattgcatg ttttccaaca acgcctccaa attcgccata
    24721 ctagcagcat atccttcttt gaaaccgagt tcaagcattc tttccaagcg ctcgagcgac
    24781 tcattgaaaa tagagatttg aactgttgtt aaaccggctt gctctgaaaa tgaaatatcc
    24841 cactcggatc caaattgatc aggattttca ttcgcatctg caaatacgtt gaaatatttg
    24901 aaatgcgttt tggggttgat gacaagatat gtttgtaata tccagcgttc ttgtccatcg
    24961 ggactcacca tggcatagaa acgctttccg ccaactgaga agtccatgta ttttgttctt
    25021 gctgtgaatg gttttggtgc attccactga tctaaaaatt cggatctggt aaaggcatcc
    25081 caaaccatat cctgcgatgc gttaaatgct ctggtgatgg ttaccgtttt cgaggccttg
    25141 tcgatggtaa gttctaatgg taaattgtca gccatgttat tgttttttca tttgttcgag
    25201 taattgatct agttgagcaa attgaccttc ccacaacttt ctgaattgtt ccaaccatag
    25261 gtcaacttct ttcatcttag atacttccag ggagtagtaa atctccctgc cgttgggagt
    25321 ttgcttgagt agctcgcatt cagtcagaat acgcaaatgt ttagataccg actgtctggt
    25381 gatgtcaaaa ttctcagcaa tggcattggg tgtcattgca tgcagcgcaa ccaaagcgat
    25441 gattgcccgt ctggtagggt ctgcaattgc ctgaaagatg tctcttctca ttattctaaa
    25501 gctaatatga agccaatcgg ctgcaaatat atatgcaact gatcagcttc gcaagtattt
    25561 taacgtttag atagagagaa taccgttgag ggtttcacgc agaggcgcag aggcgcagag
    25621 aagcagagtt acccattgcc ttcaaaaaaa ccaatcggct aattggctaa ttacttcggc
    25681 atttcctcgc tcacaatcgc tgcattcagg tattcgcgat taaggcgggc gatattggca
    25741 acgctgattc ctttagggca ttcagcttcg caggcgtagg tattggtaca agagccaaat
    25801 ccttcagcat ccatttgcgc aaccattgcc aatgcacgtt tcctgcgctc aggatcgcct
    25861 tgtggcaaga gtgcaaggtg cgacactttt gctgaaacaa ataacatagc agaagcattt
    25921 ttacatgccg ccacacatgc accacatccg atacatgccg cagcgtccat ggctaagtca
    25981 gcattggtct tgcttatggg aatggcattc gcatcctgag cctgacctgt tgatgcactg
    26041 ataaatccac ctgctgcgat aatgcggtcg aatgctgaac gatctactac gaggtcgcgg
    26101 attacaggaa atccttttgc acggaaaggc tcgatggtaa tagtttcacc atcggagaaa
    26161 ttgcgcatat acaattgaca ggtagtgctg agtttctgtg gaccatgagc acgcccgttg
    26221 atatacatac tgcacatgcc gcaaatacct tcgcgacaat catgatcaaa tgcaacgggc
    26281 tcttcgcctt tgttgacgag gctttcattt aacacgtcga tcatctcgag aaatgacatg
    26341 ttcggatttg cgtcattaac ggcatagtcg acaaacttgc cttgttcttg cgcattgcgc
    26401 tgtctccaga tcctgagttt aacgttcatg atatttattt attcaacgtt tatttcaata
    26461 gtactatttg caattttcgc aacaacaatc atgcgttatt tatatgatcg tgtagcgagt
    26521 tttactgttt cgtatttaag ttcctctttg tgcattgcag gatcattatc aacacctcgg
    26581 aattcccacg ctgcaacata ggcatattca tcgtcgttgc gttttgcttc gccttcttcc
    26641 tggaattctg tgcggaagtg accgccgcag ctctcgttgc gctcatatgc atcaacgatc
    26701 ataagttctc ctaattcaat gaaatcggca acgcgcatgg ccttttcgag atcctgattg
    26761 aactcgttag ccttgcctgt tacgcgcaca ttttgccaga attcggcacg caatttcttc
    26821 accatttcac gtgcctgctt caagccttcc gccgtgcgag ccattccgca ataatcccac
    26881 atgatcttac ctaattcttt atggtaatga tccacagatt ttgtgccttg aatgctcagt
    26941 aatttatcga gactttcacg aacatgtttt tctgtttctt caaatgcagg atgatcagtt
    27001 ggaattgctt tggtgaaaat ttctccggcg aggtatgaac ctattgtata aggaataaca
    27061 aaatatccgt cggcaagtcc ctgcatcaaa gcagaagctc ctaagcggtt agcaccatga
    27121 tcactgaaat tgcattcacc aagcgcatac aatccgggaa cggttgtcat caattcataa
    27181 tctacccaaa gtccgcccat cgtgtaatgc acagccggat aaatgcgcat cggcacttca
    27241 tatggatttt ctgccgtgat atgcgcatac atatcaaaca agtttccata acgctcttca
    27301 ataacatgtt tacccagacg attgatggct gcgctgaaat cgagatatac tgccaatccg
    27361 ctctctccta ctccacgctg atcgtcgcag gcttcttttg ctgcgcgact tgaaatatcg
    27421 cgcggagcga ggtttccgta tgatggatat ttgcgttcga gataataatc gcggtcttct
    27481 tctgcaatat ctgtaggctt tttgcggcct tcgcgaatcg ctttcgcatc ctcgcgactc
    27541 ttcggtaccc acacacgtcc atcattgcgc aagctctcgc tcatgagagt gagtttcgac
    27601 tgataatcac ctgaaacagg gatacatgtc gggtggatct gcgtataaca agggtttccg
    27661 aagaatgcac cgcgcttata tgccttccat gccgcggtaa cattcgatcc catggcgttg
    27721 gtcgataaat agaatacgtt gccatatcca ccggtacaga gcaatactgc gtgtccgaaa
    27781 tggcgttcga gttcacctgt aacgaggtta cgagcaatga taccacgcgc tttaccatca
    27841 atggttacga tgtcgagcat ttcgtggcga gaaaattctt gcacattgcc gagtccaacc
    27901 tgacgctcca atccctggta tgcgcctatc agcaactgct gtccggtttg accgcgtgca
    27961 tagaaagttc tcgatacctg cgcaccacca aatgagcgat tgtctaagag tccgccatat
    28021 tcacgggcaa aaggcacacc ctgagccacg cattgatcaa tgatattggc acttacttct
    28081 gctaagcgat aaacatttcc ttcgcgagcg cggaaatcgc cacctttaat ggtatcgtaa
    28141 aacaagcgat aagtagaatc gccgtcattt ttataattct ttgctgcatt gatacctcct
    28201 tgtgcggcaa tactgtgagc gcgacgtgga gaatcctgga agcagaacac cttcacttta
    28261 tatcccagtt caccaagtgt agcagccgca gaagccccgg ccaaaccggt tcctactatg
    28321 atgatctcca acttccgttt gttggaaggg ttcaccagct tcacggtgtt tttgtaggtg
    28381 gtccattttt cggataatgg gcctgcaggt atcttagaat ctaacataat gtaaaatgag
    28441 agatgaaaaa tgagaaagga aaaaggataa attcgtggtt tgggttattg atgtttagtt
    28501 tatacaatta tgctatccag cccatataaa tagataaagg cattgcagcg aataaagcgc
    28561 aaatcagaat gctgaatact atgccgatac ctttaattac aggcgtatat tttttgtgat
    28621 ttactcccaa agtctgaaat gcgctttgaa atccatgcac caaatgccaa cacaatgaaa
    28681 ttacaccgag tatatacacg ataagcacca caggactttg aaaagtgctg atcatcaagg
    28741 catacaaatt gagtacctct ttatcgccga tcatcgtttc gtcgagcgga ctgataccac
    28801 caatacctcc gaggcgacta tgcgtccaga aatgtgaaat atgcatcacc aaaaacaata
    28861 agatcagagt gccgagaagg gtcatgctac ggctgtacca tttgctatta gcaccgggat
    28921 tagtaacagc atatccggtt ggacgcgcag ctctattctt tgccgatagc atgaagcctt
    28981 gaacgatatg gagaatgatc cccaaaaaca acacaatctc catggtgcga ataatcacat
    29041 tcgtgcccat gaaatgcgca gccttgttga aggattctcc gccatcattg aaaaaaatca
    29101 ttgcattgat ggtacaatgg acaatcaaaa agctaatgag gaaaaggccg gtaacagcca
    29161 tcaccacctt gcgcccgatt gatgaagtca agaacgtgct aaaccaattc atttgtagat
    29221 ttttactcga ttgcaaatgt atattaggaa tgctgaatta aaaatgcatt gcctcagtgt
    29281 ttgttcacaa ccgctgtgga gtatgcttat ttagaattta tctaagtaaa agagttggtt
    29341 tcacgcagag tcgcagagcc gcagagagtg cccctcactc acccacccgc ctgaccggcg
    29401 aggcaggctc accacccgcc tgaccggcga ggcaggctca ccattcac
//
LOCUS       DAOQOU010000020        29445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_707_length_29445_cov_6.733038, whole genome shotgun sequence.
ACCESSION   DAOQOU010000020 DAOQOU010000000
VERSION     DAOQOU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 29445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29445
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_29445_cov_6.733038"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..923)
                     /locus_tag="PLM90_03175"
     CDS             complement(273..923)
                     /locus_tag="PLM90_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HMZ88400.1"
                     /translation="MKKISIMVFALVMTAGIAHAQTSDPAAVKLLKSVSQKYSGYKTM
                     QMDIALTIENLEAKTKETKNGKVSAKGNMFKAEMGNQIIMSDGKTLWTYLKDVNEVQI
                     NNFEKGAGIMTPNDIFKIAEKDYLAYMGEKVTEGGKSLQIIELTPKDKTLSFSKVKMY
                     VDPSDNSIKKGVVFDKNAIHYTYVISNFKSNVEMNDTNFKFDKSKYPGCEVIDLRE"
     gene            complement(977..3418)
                     /locus_tag="PLM90_03180"
     CDS             complement(977..3418)
                     /locus_tag="PLM90_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HMZ88401.1"
                     /translation="MAETKKSGKKAKKASNSAPFYKDERILKVTGVFLLLLSFFSGVS
                     FVSYLFTWKADQSLIWDHPWAQVFNSGITVENHLGNLGAVLSHQFFFCWFGVASFFFV
                     VWFFTLGVNFFFSRQIFNNVKLIGQGMVVMAWISTMLGFFMYKAEFPYGGGFGKGTAF
                     HLESYIGKVGAILALTAAMVVYMIIFTKFDFGFLLNMFRKKEVIPAVDPFTEDDMPVQ
                     TEEIPEVEKEMINVPPVYTSNVDITPLQGEVDDVVMELKKVEPVSEQSMPLEIEEPET
                     IVIAGDEEPLLDHQDHLSLDIETPVEEQIAAENLNYHFDPVLDLPNYKYPELDLLEEY
                     ASETVSINTEELERNKDQIIATLNNYNIQISKIKATIGPTVTLYEIIPAAGVRISKIK
                     NLEDDIALSLAALGIRIIAPIPGKGTIGIEVPNVNKETVSMKSQIGSDKFQHAKMDLP
                     VCLGKTISSENYIADLTRMPHLLMAGATGQGKSVGINAILISLLYKKHPSQLKFVMVD
                     PKKVELSLFSSIEKHFLAKLPGEEDAIITDTRKVVNTLNALCIEMDTRYELLKNAQVR
                     NIKEYNAKFMKRKLNPKEGHMFLPYIVLVIDEFADLIMTAGKEVEMPIARIAQLARAV
                     GLHCVIATQRPSVNIITGTIKANFPARIAFKVASKVDSRTILDNSGAEQLVGRGDMLL
                     AIGSDMVRLQCPFVDTPEVDKVVDFISNQIGYPEAFMLPEFIDEQAQKEKMEIMDGER
                     DPLFAEAAKIIVQHQQGSTSLIQRRLKLGYNRAGRLMDQLEAAGIVGQSAGSKPREVL
                     YSDLAQLEQYLQTLD"
     gene            3841..4884
                     /locus_tag="PLM90_03185"
     CDS             3841..4884
                     /locus_tag="PLM90_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HMZ88402.1"
                     /translation="MSHKPVEYTEYLQLDKILNAQSLESDKAGAHAHDEMLFIIIHQA
                     YELWFKQVHFEVDSALQVMGKPEVNDNSPELQTVVHRLDRVVTILKVLVHQIDILETM
                     TPLDFHEFRDFLRPASGFQSWQFKMLEAKLGLKFDNRYGKEYYTSMLKPQYVDLVKDV
                     ESQKSLLEMLDDWLARMPFFSDDNLWKSYQPASQNDSTHHVFWNDYLNAYSKTLVEGE
                     QHNKEIFASIFLGEGKSEHTSLSNKAMRNALFISLYRGYPLMHLPFQLMQHLLDIDEQ
                     MATWRFRHINMVHRMIGSRVGTGGSSGKEYLQGALNKHYIFKDFAVLSSFLIQRNKLP
                     HLSSELNHKLGFM"
     gene            4966..5457
                     /locus_tag="PLM90_03190"
     CDS             4966..5457
                     /locus_tag="PLM90_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88403.1"
                     /translation="MKIFLTILTCGLIVQAQAQTIVPISSAEQSYEVLDLTKEAKITW
                     GGYEEIGVATRSETDGALNSKAIVVAVGDNPGFDNKPYAAQVCENSTAGGHDDWYLPA
                     KDEAALIYLNIDKFNWSDSYTLWTSTEAAGTQAVSLYTYNGTWYNVQKVDSYNMVCMR
                     KTK"
     gene            complement(5487..6629)
                     /locus_tag="PLM90_03195"
     CDS             complement(5487..6629)
                     /locus_tag="PLM90_03195"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88404.1"
                     /translation="MRKLISFLSCIFLAGIMHAQVTIIDSIYSGGMYRNYRMYIPTAY
                     DGSEAVPLLFNLHGYSSDNFGQALYANFNPIADTAGFITVLPNGTFDAFGLRYWNCFV
                     APGIGVDDVQFLSDLIDSVAAEYNIDLNRVYSTGMSNGGFMSYTLACELSERIAAIAS
                     VAGTIDKDRLSYCNAQHNVPVLHFHGTADPTVPYGDNLAFFSVDSVINYWVAHNHTDA
                     TPVFTEVPDIVTTDGCTAEHYVYGNGWTDSRVELYKIIGGEHTWPGTAITFLGITNQD
                     VNACKEIWRFLSQYRLDELSSVDPLTDNDLVQINPNPADQFLVINSKFSINGVEIINM
                     NGQIIKQFNPAGATQVNIPLSELAQGLYLIKISTGDTYITQRFIKQ"
     gene            complement(6654..7175)
                     /locus_tag="PLM90_03200"
     CDS             complement(6654..7175)
                     /locus_tag="PLM90_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88405.1"
                     /translation="MDWVIETPRLLLRELNTDDAQFFFDLNNDPEVIRYTGDVAFESI
                     DAARQFLAGYDHFKKYGFGRWAMATKSEHKLVGWCGLKYSSDANEYDLGFRLFKNEWG
                     KDFATEAGIACLTAGFQKFNMPMIVGRALKANIGSIRVLEKCGMRYWKDVEEHDNEAV
                     VYRCFPEDLNNLL"
     gene            complement(7177..8106)
                     /locus_tag="PLM90_03205"
     CDS             complement(7177..8106)
                     /locus_tag="PLM90_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88406.1"
                     /translation="MHKLSTYSFGIVFSLLLLLLPSWASAQSRTQQFNQLLDKNDTAR
                     AQVLLTQWKKDVPGDPEWYVARYNFLVWSSMKSIVTIGSNPKGNNYMEITPTDTSVDA
                     TPQFMYADVYYQPKQVASAIACIDSAIALFPDRLDLRFGKIYLLGETQSYQAFADEIV
                     KTIHYGATFNHNWKWTDGLAIDGGIASFNESIQSYIVQLYDTNVDSLGVQIGQIATAV
                     LQYDPNNIENLSNLAISYMLASNYEEALTPLLKAETVDPTDAIVLSNIAFCYHRINNK
                     EQAIAYYEKSIKYGDREMKAFSYEQIQLINSEK"
     gene            8299..9927
                     /gene="pruA"
                     /locus_tag="PLM90_03210"
     CDS             8299..9927
                     /gene="pruA"
                     /locus_tag="PLM90_03210"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HMZ88407.1"
                     /translation="MSNAIFRIPKALNEPVKGYAPGSPERKALKQAIADLKAISVDIP
                     MFIGGKEVRTGKTVAIHPPHEIAHTLGHYHQGGAEHVKMAIDAALTAKQAWENTPWED
                     RAAIFLKAAELLAGPYRYKMNAATMLGQSKNPFQAEIDSACELIDFWRFNAEFMQQVY
                     SDQPESAPGIWNRMEHRALEGFVFALSPFNFTSIGGNLPTAPAMMGNTVVWKTSNTQI
                     YSAWVIMEILREAGLPDGVINLVHVSGSEAGKVIFSHPDFAGIHFTGSTEVFRTIWKT
                     IGDNIHVYKTYPRIVGETGGKDFVIAHPSAHPKQVATALSRGAFEFQGQKCSAASRAY
                     IPSNMWEEVKRYLVTDLSEMKMGGIEDFSNFINAVIDEKAFDSISGYIAKAKADGQEI
                     IAGGTYDKSKGYFIAPTVILAKDPSYRTMCEEIFGPVLTIHVYDAEKWSETLQVVNNT
                     SPYALTGAIFSQDRYAVVEATVALRHAAGNFYINDKPTGAVVGQQPFGGARASGTNDK
                     AGSILNLLRWVSPRTIKENFVPPVDFRYSFLNEE"
     gene            9945..11183
                     /locus_tag="PLM90_03215"
     CDS             9945..11183
                     /locus_tag="PLM90_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ88408.1"
                     /translation="MMQRYAGIITSILFSILGSSSMVHAQSYDAAPDFYEINEDVPDV
                     TMDVMANDLNTTGVTDVYLNIATLPLHGTVTVDAVNDLLIYSPNDDYYGTDNFEYTGC
                     ANTDPDICGTTSVIITINPVPDKPIAVDDAVETYVNTPLNIEPLLNDIDVDNEGLEFT
                     IIDDADYGATTIIFGEAVFYEPFPCVYGEDCFIYKICKIGSDIYCDTATVCVDVQTTN
                     FNPPSALNDTATVVVIDTIDIPVLLNDFDGDGDAMIVSEIIADGMTGTATINADNTIK
                     YIPFVVGTDDFRYVVCDNNCPPYCDTAYVHMKIEEPEIPAEAIHVPNSFSPNGDGIND
                     MLTIEGLESGIKYNVNIYDRNSSLVFEKEKLATSDVWDGTSNVEFISSSGNVPEGTYF
                     YVIRIEGLPDPLSGFIVVKR"
     gene            11180..12139
                     /locus_tag="PLM90_03220"
     CDS             11180..12139
                     /locus_tag="PLM90_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ88409.1"
                     /translation="MKKLYQSTLLLLMLCSANALYAQLPPLFEQYHLNQLVFNPAYAG
                     SKGGIEAGFFLHRHSTNVQGAPGTESFTLHTPLANDNVGLGLKIYHDKLGVTNNSFVG
                     LDYAYRMHISDHLTAAIGIEGSIANYKVDYSQLDAFINGDPTFTKASESYWSPDAGLG
                     VYLHSDIYYVGLSVMSLSGLSANGNDNPDVAQDEKFDKVATVYGTLGTLIKINDKLTI
                     KPDLLIKITDSIPAQMDVNLNFIYNNVLLVGGGYRTNGSISFVGQYIFSTDNKITGHE
                     AGLGYSYNTMLADDKVFISPSHEVFLTYRFYKHNNKFVNPRFF"
     gene            12139..13644
                     /locus_tag="PLM90_03225"
     CDS             12139..13644
                     /locus_tag="PLM90_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88410.1"
                     /translation="MRMQAIYRYCLIIILFSGISPLHAQNEILAKADKYYNNGIYPEA
                     AALYIDHLEQQYDYIVNVKLADCYYRMEEWSEAEHWYGVIVSHNIADAGTLLRYATLL
                     KMNGKYRSAKTYFLKYAAYKPDGYYLASTCDWALANMNKPSQYYIDTLPFNTKGSEMT
                     PTIYKNGIIFAKSNGTKINNNTGLPYYDLYSADVRTDGSWITNKLDHVNSPYHDAAPY
                     YDGASKGLYFTRSNHYKNRTVVSIDGQVRLELFYAPYVDSKFGTPHPLNVNSKIYSVG
                     HPATTPDGNILIFASDKQGGKGGTDLYYCMKRNGNWSEPKSMGDVINTAGDELYPFMT
                     ADGTLYFASNYHPGFGGFDLFKCEREGDHWSTPENLGMPVNSPQDDYGFTMKNGVGYF
                     SSNRPGGMGGDDIYQVTQMAQIASVFVYDNNKNAIYKARVTFFESPNSQVICETDPNG
                     YGDVSTLSGVSTSIKISKEGYLDKVINNLGGLRSSNGVLPVELTPLIGGNE"
     gene            13713..14555
                     /gene="surE"
                     /locus_tag="PLM90_03230"
     CDS             13713..14555
                     /gene="surE"
                     /locus_tag="PLM90_03230"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMZ88411.1"
                     /translation="MQQNNNKPIILITNDDGIHAPGIHALVEAMRPLGTIVVVAPDKP
                     QSGMGHAITLNEPLRLKKSNLFGDIEAYACTGTPVDCVKLAKDKILHKKPQLCVSGIN
                     HGSNSSINIIYSGTMSAAMEAGIEGIPAVGFSLLNYAIDADFTSAAHYAAKIAEQVLQ
                     HGLPQNTLLNVNIPYLPIDEINGMRICRQAHAKWEEEYDERLDPHGRPYYWLTGNFVN
                     YDGGDDTDEWALSHGYVSIVPVQYDLTAHQAIAFFHKNWSLYQTKSPHDIHLDNSDTF
                     NDLG"
     gene            14561..14878
                     /locus_tag="PLM90_03235"
     CDS             14561..14878
                     /locus_tag="PLM90_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88412.1"
                     /translation="MSKLQEDNVFVGMAVAVFFMVTTYYAFFQLNESLSGNITIKGAI
                     FTGVTERFIATLAVFFNILPFVIYMRVRKDNCMRGVGIITVLSAMFILVYFYIQHHST
                     IFQ"
     gene            14887..16002
                     /gene="lpxB"
                     /locus_tag="PLM90_03240"
     CDS             14887..16002
                     /gene="lpxB"
                     /locus_tag="PLM90_03240"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMZ88413.1"
                     /translation="MRYYLIAGEASGDLHGANLVRALSAKDPDAVFRGFGGDAMHEAG
                     VDIVTHYKETAYMGFVEVFMHLRKILNNIKSCKQDITGWKPDVVILIDYPGFNLRIAP
                     HVHEIGIKVFYYISPQLWAWKSSRVNIIKQYVDRLFVILPFEEAFYQKFNYPVSFVGH
                     PLLDAIASNKGNEHFREQFQLGDKQLIAVLPGSRKQEITVKLPVMLSMAKYYPEFVFV
                     VACAPGMDDSFYAGFEKRENIIYISGSTYDILRNAYAAMVTSGTATLETALFRVPQVV
                     CYKGSVISYHIAKRLVKIKYISLVNLIADKQVVPELIQHDFNHDSLRTHLDLLLKDAA
                     YRNTMLSEYDKLYTVLGGVGASARTADEMMKLLAEGK"
     gene            complement(16032..16105)
                     /locus_tag="PLM90_03245"
     tRNA            complement(16032..16105)
                     /locus_tag="PLM90_03245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16069..16071),aa:Ala,seq:ggc)
     gene            16181..17047
                     /locus_tag="PLM90_03250"
     CDS             16181..17047
                     /locus_tag="PLM90_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ88414.1"
                     /translation="MHDHPVDILRLELAQPRLQGNKFYKLKYNIERFKQENCDTLLTF
                     GGVFSNHIHAAATACYDHGIRGVAVLRGEDDPFNPTLKFIRSMGMETIFVSRELYRQR
                     NNSDFLHHFSIDANKIYVLPEGGTNEFAVRGCSEILGDLADKYDRIFCAVGTGGTLCG
                     LICSPGLSATVTGISSLKADKDSLTPYIRQLCGDAGNKTQWDINFQYHFGGYAKYNDT
                     LMAFVEQFEHKYNIKPDPVYTAKVFYAVNDMISNKSISASERILILHTGGLQGWDGWH
                     YRFHHPKKQSHA"
     gene            17124..18884
                     /locus_tag="PLM90_03255"
     CDS             17124..18884
                     /locus_tag="PLM90_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88415.1"
                     /translation="MWRFIISPNDVMVSGRNDGLKNFFTLQAYLEQPASDGYIDFTAM
                     QYPYGDAVWYTDNSPFIAMPLRFIQLHFGNIAPYTVAIIQWIFLLGILLTPLTVIPLL
                     RRFLTNEWLIAIGAIVITWTSPQFLRFFVGNYNLSLTPVYFLVMALLLRIYDNFQSRN
                     RKKLLLHAALIAIVIFLASGIHLYYVILLGLPAIAFLGILFLQQIRKSAEVLYFTAIP
                     SFAIAIATFGMMLLINTTDPWRSMRPTQLDGSNISAWEMRFMHLYKARDEVNLVPFIG
                     GKIHFDPENGVYLGGFFWYLLLMVFILIAFRFFRYRDVSFIKTLKPSPVGWIFILTAI
                     VAFFAGTGMHLHLIGRGNTMENIFNPLYYIGKIYPGVSQFRCIARMGWWLFYAAQIGI
                     LVAVERIILNQKKWIVNAVLCIGLSLLATDIYGIKQFTNSRFYPNYFNQKSLASLPDL
                     DYDAYQAILPIPYYHVGCEEYDFTIDDANEWSRYTYQLQLKSHLPLMSVKLSRTPQLF
                     ASQMMQLLLEPQSPGTLHEYLNDKPILVIYDPSIEEKQNLEPAATVLKESPGLITSEH
                     MVLIGSKGNVQYYAWYLKSN"
     gene            18913..20280
                     /locus_tag="PLM90_03260"
     CDS             18913..20280
                     /locus_tag="PLM90_03260"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HMZ88416.1"
                     /translation="MDFINNYIETNKERLLDELFALLRIPSVSADPKYKADVARTAHA
                     VADSLRAAGADNVRVNATEGHPIVTAEKIINPSLPTVLCYGHYDVQPADPVDLWDSPP
                     FEPVIKNGNIYARGACDDKGQVYMHVKAFEAMMQNNALPCNVKFIIEGEEEVGSPNLG
                     TFIKENKDLLSCDVILISDTSIIANDVPSVTVGLRGLTYMEVEVTGPNRDLHSGVYGG
                     AVANPINTLCDMISSLIDKDGHITIEGFYDDVALISDSERAEMAKTPFSLDEYKQDLA
                     INDVKGEKGYSTLERSSIRPTLDVNGIWGGYTGEGSKTVLPSKAYAKISMRLVPDQSS
                     EKIAELFDKHFNKIAPPYVKVKVTHHHGGEPVVVPLDFPPMQAAIMAMEKTFGKKPIP
                     TREGGSIPIVAMFEKVLGAKSILMGFGLNSDAIHSPNEHYGLFNYYKGIETLPWFFKY
                     YSELK"
     gene            complement(20351..21889)
                     /gene="gpmI"
                     /locus_tag="PLM90_03265"
     CDS             complement(20351..21889)
                     /gene="gpmI"
                     /locus_tag="PLM90_03265"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMZ88417.1"
                     /translation="MKSMKKVMLIIMDGWGHGQNAAASAIAQANTPNVNAYYKRYPNA
                     ELRTDGEFVGLPEGQMGNSEVGHLNLGAGRIVYQELQRIHMAIRNGELASNNTLLSAL
                     DFAKNNGKKVHFIGLVSDGGVHSHSNHLMALCDIAKSKGLTSDQVFIHAFTDGRDCDP
                     KSGVGFIRTLQQHLTNSAGTIASVCGRYYAMDRDKRWERIKKAYDLLVHGEGNATQDV
                     IKSIEQSYSDNVTDEFILPIICTDAQQKPLATIAAGDVVICFNFRTDRCRQITRALTQ
                     EAFPEFDMTPIDLHYVTMTRYDHTFKNVGVIFESDDLSKTLGEILEANNKTQIRIAET
                     EKYPHVTFFFSGGREKEFSGERRIMIASPKVATYDLQPEMSAYLVKDAIVEAINNESP
                     DFICLNFANADMVGHTGVFSAAIKACEAVDTCVGEVIEAALSKNYAVILTADHGNSDV
                     EINEDGTPNTQHSTNPVPVFYIDKEPAFQQIRNGKLGDIAPTILTLMGIDIPKEMTGE
                     ILVS"
     gene            22148..22549
                     /locus_tag="PLM90_03270"
     CDS             22148..22549
                     /locus_tag="PLM90_03270"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HMZ88418.1"
                     /translation="MFKNNRTKLIAILAVVPFLIFATMQSELAGITSAIKSGDSKSLA
                     TYFDNTVEVKIANKEGAYSKSQAEAVVKDFFTKNPPKDFSFLHEGTSGGSNGHYAIGS
                     LSTDKGKFRTYVYMKKRADKFYIQELSFENE"
     gene            22542..23399
                     /gene="nadC"
                     /locus_tag="PLM90_03275"
     CDS             22542..23399
                     /gene="nadC"
                     /locus_tag="PLM90_03275"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMZ88419.1"
                     /translation="MSSVEIWDIIDFIDEALIEDIQEGDHTTLATIDHQAAGTAKLLV
                     KEPGIIAGVELAEQILRHVNPELEVDIFIPDGARVEPGDVVFHATGRVQALLVAERTM
                     LNFMQRMSGIATTTNLFVQTVRHTGAKILDTRKTTPGLRTFEKWAVRIGGGHNHRYGL
                     YDMILIKDNHIDFCGGITPAILKVKEYLRDNDLNLRIEVETRNLEEVAEVLRTGHVDR
                     IMFDNFDVDTMRQAVIEVAGQFETEASGGVNLTTVQAIAETGVNFISVGALTHSVKSL
                     DLSFKALIH"
     gene            23449..25380
                     /locus_tag="PLM90_03280"
     CDS             23449..25380
                     /locus_tag="PLM90_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ88420.1"
                     /translation="MKEQLFHIEILLKRLGILLLLYMVCRILFFLFNMALFPHIPPGE
                     FLQLMLYGLRFDLSSIIYVNCIFIVLHVVPSPWFDKNWYQRMLKIIFYSFNGVGLLLE
                     SGDWIYYEYGLKRTSTHELGLTGDTNILPQVIKDYWIIFVIAFILILGVEYLYRKTEL
                     HRLKKVHFKLPRFIHYPYQIIIMVVVLTFSVIGARGGIQPEPISPEIATDYVDDGRLT
                     SLIINTPFSVIYAFGHRQLVEPVYYEDEQELAKQFNIFHDNALYYQWPDSIAKPSKPE
                     NVCVIVLESFSKEYIGYFNHGHGYTPFLDSLLTVGMAFDNAYSNGKSSNQGIVAVTSG
                     IPVLMEEPFISSIYRNNKFEGVGTLLKDVGYSSYFFHGANNGSMQFDRFMAQAGFDGY
                     FGRNEYGNDADFDGNWGIFDEPFLKWTANKMTTLKAPFYSEIFTISSHHPFTVPAQHV
                     GQFPKGPIPMLEVVGYADYALQQFFEEAKKQPWYNNTLFIITADHPGPPLPEHEFYQE
                     QVGAHSTWLLMYKPNGQFKGVNSEVVQQSDIMPTVLDYVGYKGKYMAFGNSIFDTTAA
                     RYAYNYHTSDYMLLDKDFALIFNGDRPVGLYDYKIDSTLSNNQMHEFPDITASMEDRI
                     KAIIQIHHHAMINNKLVAE"
     gene            25377..26264
                     /locus_tag="PLM90_03285"
     CDS             25377..26264
                     /locus_tag="PLM90_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HMZ88421.1"
                     /translation="MKKHILFIINPVAGVKRKDRIPRLIDKYLDHSHFDYEVIYTERR
                     GHATQIASEAAARGVHVVAVAGGDGSVNEAATGLIGTNTCLAIIPSGSGNGLARHLGY
                     SINIRSTINIINAYHVRKIDVCKVNGTMFFSLIGIGFDAFVAKVFSREETRGFLTYAW
                     SAIKSVSAFDAFEYSANLADRKIGGKAFMINVCNSNQYGYNVKVAPHASLSDGMLDVI
                     VLGDIPKWKIPLVVLQVFTRTHMHTKHFTIYKTPSLTIDTPQYAHLQIDGETAPKDKS
                     FKIEIMPEALQVLMHPDND"
     gene            26268..26609
                     /locus_tag="PLM90_03290"
     CDS             26268..26609
                     /locus_tag="PLM90_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HMZ88422.1"
                     /translation="MAKQKLNLSGLVYSTNPDYKPESSEENQETLAPAQQNLRVWLEK
                     NHRGGKTATVIRDFVGNTDDLEALCKMLKSKCGTGGSAKDGEIIIQGDHRKKIGEILG
                     GLGYKYKLAGG"
     gene            complement(26614..26985)
                     /locus_tag="PLM90_03295"
     CDS             complement(26614..26985)
                     /locus_tag="PLM90_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HMZ88423.1"
                     /translation="MFKPVIDFHQSKHAMATYSDFEKIEIRTGTIISAEPFPAARKPA
                     YQLTIDFGALGIKRSSAQITKHYTAESLIGKQVVAVVNFPVKQIANFFSECLVLGAIE
                     ADGNVVLLQTERTVKNGLRIA"
     gene            complement(26991..27899)
                     /locus_tag="PLM90_03300"
     CDS             complement(26991..27899)
                     /locus_tag="PLM90_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88424.1"
                     /translation="MKTTHLFALVLILFASMRASQAQITVFEYSKKEMAHNLSELKKR
                     NLIFVIRDSEIEQIPKYETALRNGWTLSEVEVVTSDELLYYEDSENYTFVTLDARAAV
                     AGKADMSTAVLTMMFKDSVTKKGPTNIKLAEIFMYADYKYFNTTYKDIITKEDSVIQI
                     HNYLPGFLQLYFQYIQKNLETGTSVKHFQKTETLNSNKTALFKNVLYIPDYCLKKFKL
                     IDPRANEYYEESEFRDKYFGKLTFVDSDSLSDLILRKEINFVLIYNMHYTQSFVNIFN
                     VQTGEMVHKNVYTGDYDLAPVRLKEI"
     gene            complement(28117..29304)
                     /locus_tag="PLM90_03305"
     CDS             complement(28117..29304)
                     /locus_tag="PLM90_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ88425.1"
                     /translation="MTSLRAQQTWIRTGITDTILAIHGDGTLIWAATEADGVKSFNPA
                     TGAITTYNSAKSGFPDNDFRSILCVNGDVYAGSNNYGLFILHDEGWSSFDTTNSDIIG
                     NSIKDIMYVEADSSIWFATDKGLSILKEGTWTNYDSIASPLAGNALTCLYQSREGIIW
                     IGTKYAGLSKYDNGTWETFNYGNSGINNNAIRAITQDEDDLLYVADYLGVNRYDIVAD
                     NWLFVYNLFTVPMTSEKVNRMGFDDDDNFWMATHGGVTRADSTNHFTLYYDYNSNLPN
                     NVCDALYIDENNTVWVGTYGGLAFYTQEAFPNTITSSSMSIIPNPANTDITVTINQLA
                     EDIPTFCIYDLNGKNLENVIGVPALYGECAYTINISDLPSGMYLLCAIQSNERMFETF
                     VKL"
BASE COUNT         8535 a         5832 c         6131 g         8947 t
ORIGIN      
        1 gaaggatggg acggcacatt taatggcgaa tctttgaaca atggggttta tgcctatatt
       61 atggagctca catttgaaaa cggtatcact acgcaatatg caggtaatat cacgcttata
      121 agataattgg cctctacgct atttcagaaa ggctgtctgt catggcagcc ttttttgttt
      181 gcagaaaaca tttgaaccgc tcaaccacaa tttaatatgc ataaaaaaag gccgcctgtg
      241 tgcagacgac cttttcgata tgtctaaaaa atttattctc ttaaatcaat tacttcacaa
      301 ccgggatatt tcgatttgtc gaatttgaag ttggtatcgt tcatttcaac atttgatttg
      361 aaattgctga tcacgtatgt ataatgtatc gcattcttgt cgaatacaac acctttttta
      421 atcgaattgt cgcttggatc aacatacatt tttactttgc taaagctcaa tgttttgtct
      481 tttggtgtaa gctcaattat ttgcaaagat ttaccacctt cggtcacctt ttcacccata
      541 taagcaaggt aatctttttc agcaatttta aagatgtcgt taggagtcat aataccggct
      601 cctttttcaa aattattgat ctgaacctca tttacatcct ttagataggt ccaaagtgtt
      661 ttaccatcag acataatgat ctgatttccc atttcagcct tgaacatgtt tcctttagca
      721 gaaactttac cgtttttagt ttcttttgtt ttagcctcaa ggttttcgat ggtaagggca
      781 atatccattt gcatggtttt atacccacta tatttctgac taacggattt cagcaattta
      841 acggctgctg ggtcggaggt ttgagcatgg gcgatacccg cggtcatcac aagggcaaac
      901 accataatac tgatcttttt catgacagtg tggattggaa gtgcaaagtt aatttgaaaa
      961 caatggatta ttaagtttaa tccaaggttt gcaaatactg ttccaattgt gccaaatcgc
     1021 tgtataaaac ttctctcggc ttgcttccgg cactctgacc aacaatgccg gctgcctcca
     1081 gctgatccat caaacgcccg gccctgttgt atcctagctt gaggcgacgt tgaataagcg
     1141 aggttgatcc ctgttggtgt tgcacaataa tttttgcagc ttcggcgaac aacgggtcgc
     1201 gctcgccgtc cataatctcc attttttcct tctgggcctg ctcatcaatg aactccggca
     1261 acatgaatgc ctccggatat ccgatctggt tggaaatgaa gtctaccacc ttgtcaacct
     1321 caggggtatc aacgaacgga cactgcaatc gcaccatatc gcttccaatc gccaacaaca
     1381 tgtctccacg tcctaccagc tgttcggctc cactattgtc aaggatggtc ctgctgtcga
     1441 ccttactcgc cactttgaaa gcgatacgcg ccgggaagtt ggccttgata gtaccggtaa
     1501 tgatgttcac cgatggacgc tgggtggcga tcacgcaatg caatcccacc gcacgagcaa
     1561 gctgggctat acgcgcaata ggcatctcca cctcttttcc tgccgtcatt ataagatcgg
     1621 caaactcatc aatcaccaat acaatatagg ggagaaacat atgcccttcc ttcggattga
     1681 gcttgcgctt catgaacttg gcattgtatt ctttgatatt gcggacttgc gcatttttca
     1741 acaactcgta acgggtatcc atttcaatac acaaggcatt tagcgtgttt accaccttac
     1801 gggtatcggt gataatggcg tcttcctctc cgggaagttt agctaaaaag tgcttttcta
     1861 tggatgaaaa caaactcaat tccacctttt tgggatccac catcacaaac ttgagttgcg
     1921 atgggtgttt tttatataac agtgaaatga gtatggcatt gataccaaca gattttccct
     1981 gaccggtagc acccgccatc aacaagtgcg gcatgcgtgt aagatcggca atgtagttct
     2041 cgctgctgat ggttttaccc aaacataccg gaagatccat tttcgcatgc tgaaatttat
     2101 cactgcctat ctggctcttc atcgaaactg tttctttatt cacattcggc acttctatac
     2161 cgatggtacc ttttcccggg attggagcga tgattcgaat gccaagcgct gccagactta
     2221 atgcaatatc atcttccaga ttcttgattt tgctgatacg cacacctgca gccggaatga
     2281 tctcatacag tgttaccgtt ggtcctatcg ttgcttttat tttgctgatc tgaatattat
     2341 aattattcag tgtagcaata atctgatctt tatttctttc gagctcttct gtattaatgg
     2401 aaaccgtttc actggcatat tcttccaata aatctaattc aggatattta taatttggaa
     2461 gatcgagaac agggtcgaaa tggtaattaa gattttccgc tgcaatctgc tcctccaccg
     2521 gtgtttcaat atcgagtgat aaatgatcct gatgatctag tagaggttct tcatcgcctg
     2581 caataacaat tgtttcgggc tcctcaattt ccaaaggcat agattgttcc gataccggct
     2641 caaccttttt caattccatt accacatcat caacctctcc ctgtaacggt gtaatatcta
     2701 cattagatgt ataaacggga ggaacattga tcatttcttt ttccacctca gggatttcct
     2761 ccgtttgaac aggcatatca tcttcagtaa aaggatctac agcaggaatt acttctttct
     2821 tcctgaacat attcaacaaa aatccaaaat caaattttgt gaagatgatc atatatacta
     2881 ccatcgccgc agtaagtgct aaaatggcgc ctactttgcc gatataactc tccaaatgaa
     2941 atgctgtccc tttgccgaat ccaccgccat aaggaaattc cgctttatac atgaaaaaac
     3001 cgagcatggt gctgatccat gccattacta ccattccctg ccctatgagt tttacattgt
     3061 tgaatatctg tcggctgaaa aagaaattta ctcccagtgt aaaaaaccaa accacgaaaa
     3121 agaaactggc tacaccaaac cagcaaaaaa agaattgatg actcaatact gctccaagat
     3181 tgccgagatg attttctact gtaattccgc tattgaaaac ttgcgcccag ggatgatccc
     3241 agataagact ctgatcagcc ttccaggtaa acagatagct gacaaacgat acgccgctga
     3301 aaaaactcag cagtaataaa aatactcccg taaccttcaa tatgcgctca tctttgtaaa
     3361 atggagcgct gtttgatgct ttctttgctt tcttacctga tttctttgtt tccgccatgt
     3421 gaaaatttgc tatgatcttt gaactcagct cgcaattgca tttgttgtct tttctgtccc
     3481 attcctgttt ttctttccta cccgaattgt attacaattt caggtatttg ctgcttcagg
     3541 ggggcttggt taaagggcaa atgcttgtgc aaaaaacacg atgctatttt tgaaatgagt
     3601 ttttctcaaa agtaaaggta ttgatatcca cctcccttaa gaacgcgttt atacacgaag
     3661 ttggtctgaa aatgactagg attttggata gtgcggccgt agtttcaaaa ttggccatcc
     3721 gtcagaacca aaggtggagg tatcttattt tagtcatcga tatctgtcca tggatagggt
     3781 ttgagcagtg tcatcggtca ttcaccccgc attatgtatt tttgacgcaa atagcaggaa
     3841 atgtcacata agcccgtaga atataccgaa tatctccagt tagataaaat actgaatgct
     3901 caaagccttg aaagcgataa ggccggagcc catgcacatg atgaaatgct gtttatcatc
     3961 attcatcagg cctatgaatt gtggtttaaa caggtgcatt ttgaagtaga tagcgcgctg
     4021 caggtgatgg gtaaacccga agtaaatgac aatagtcccg aattacaaac tgttgtacat
     4081 cgcctcgata gggtagtgac cattttaaaa gttttggtgc accagatcga tattctggaa
     4141 acaatgacgc ctctcgattt tcatgagttc cgcgattttc ttcgccccgc ctccggattt
     4201 caaagttggc aattcaaaat gcttgaggcg aaactgggtt tgaagtttga taaccgctat
     4261 gggaaagaat attatacaag catgctaaag ccgcaatatg tcgatttggt gaaagatgtg
     4321 gaatcacaaa aatcactgct cgaaatgctc gacgattggc tggcacgtat gcctttcttt
     4381 agtgacgaca atctttggaa gagttatcag cctgcatcac aaaatgacag cacgcatcac
     4441 gtattttgga atgattatct gaatgcctat agcaaaacct tggttgaagg agaacaacat
     4501 aacaaagaga tatttgcttc tatttttttg ggcgaaggga aaagtgaaca tacttctcta
     4561 agtaataagg ctatgcgcaa tgccttgttc atttcgcttt atcgcggata tccactcatg
     4621 catttacctt ttcaattgat gcaacatttg ttggatattg atgaacaaat ggctacatgg
     4681 cgattccgtc atatcaatat ggtgcaccgt atgataggaa gtagagttgg cacgggcggc
     4741 agctcaggaa aagaatactt acaaggagca ttaaataagc attatatttt taaagatttt
     4801 gctgtgctga gcagcttcct gatccagcgg aataaattgc cccacctaag tagcgaatta
     4861 aatcacaaac tgggtttcat gtaaatgact gcatgtcgaa atttaatatt ttgttatatc
     4921 tttagtagac aaccctttat ttaacccaat tcaaatccgt atcaaatgaa aatctttctg
     4981 accatattga cttgcggctt aattgttcag gctcaagctc aaactattgt tccaatcagc
     5041 agcgctgaac aaagttatga agtgctcgat cttactaaag aagcaaaaat tacttggggt
     5101 ggttatgaag agattggtgt ggctactcgc agtgaaacgg atggggcatt aaattcaaaa
     5161 gctattgttg ttgcggtagg cgataatccc gggtttgaca ataaaccata tgcggctcaa
     5221 gtttgtgaga atagcacagc cggcggtcat gacgattggt atctgcctgc caaagatgaa
     5281 gcagccttga tttatttgaa tatcgataaa tttaattggt cagacagcta cacactctgg
     5341 acttctaccg aagctgcagg aacacaagct gtttcattgt acacttataa tggcacttgg
     5401 tataatgtgc aaaaggtgga tagctacaat atggtgtgca tgcgcaaaac aaagtaatcg
     5461 cgcacaaaaa taacctggtt aaaatattat tgtttgataa atctttgggt gatgtaagta
     5521 tcgcctgtgc tgattttgat caaatataaa ccttgcgcta attctgaaag tggtatgttc
     5581 acttgagttg cgccggcagg attgaattgt ttgattattt gaccattcat gttaattatt
     5641 tcaacaccat tgattgaaaa tttggaatta ataacaagaa attgatcagc gggattcggg
     5701 tttatttgaa caaggtcatt gtctgttaaa gggtcaacag aacttaattc atcaagtcga
     5761 tattgactta aaaaacgcca gatctcttta caggcattca catcctgatt ggtaattcca
     5821 agaaaggtga ttgctgtgcc cggccacgtg tgttcaccac caataatctt gtataattct
     5881 actctgctgt ctgtccagcc attgccataa acataatgtt cggcagtgca gccatccgtt
     5941 gtcacaatgt ccggtacttc tgtgaataca ggtgtggcat ccgtatgatt atgcgctacc
     6001 cagtaattaa ttactgaatc caccgaaaaa aatgcaaggt tgtcgccgta gggcaccgtc
     6061 ggatcagcgg tgccatgaaa atgtaaaaca ggaacattgt gttgcgcatt gcaataggag
     6121 agtctgtctt tgtcaatagt tcctgccacg ctggctattg ccgctatgcg ctcgcttaat
     6181 tcacatgcca acgtataact catgaaacca ccgttgctca ttcctgtaga atagactcta
     6241 ttgaggtcga tattatattc tgcggcaaca gaatctataa ggtctgataa aaattgcaca
     6301 tcgtcgacac caatgccggg tgcaacaaaa caattccaat agcgtaaccc aaacgcatca
     6361 aaagtgccat tcggaagcac agtgataaaa cccgctgtgt cggcaattgg attaaaattt
     6421 gcatacaatg cctgaccgaa attgtcggaa ctgtaaccat gtaaattaaa taataagggc
     6481 acagcctcag atccatcata tgctgttggt atatacatgc ggtaattgcg gtacatgcct
     6541 ccactataaa tgctgtcaat aattgtcacc tgtgcgtgca tgattccggc aagaaaaata
     6601 caggataaaa aactgatgag tttacgcata ttgatttatt tctttaaatg atatcacaat
     6661 aaattattta aatcttcagg gaagcatctg taaaccacgg cctcattatc gtgctcttca
     6721 acatctttcc agtaacgcat accacatttt tccaaaactc tgatagagcc tatattcgct
     6781 ttcaacgcac gaccgacgat catcggcata ttaaattttt gaaagcctgc tgttaaacat
     6841 gcaattcccg cttccgtggc aaaatctttt ccccattcat tcttaaatag ccgaaatccg
     6901 aggtcatatt cattcgcatc agaggaatat ttcaatccac accagccaac aagcttgtgc
     6961 tctgattttg tcgccattgc ccagcgacca aaaccatatt ttttgaagtg atcatatccg
     7021 gccaaaaatt gcctggctgc atctatactt tcaaatgcaa catcgcctgt gtatctaatc
     7081 acttcgggat cattatttaa gtcaaagaaa aattgtgcgt cgtcggtatt taattcgcgc
     7141 agcaataatc ttggagtttc aatgacccaa tccatactat ttttccgaat taattaattg
     7201 tatttgttca taggaaaacg ctttcatttc cctatcgccg tatttgattg atttttcgta
     7261 ataagcaatc gcctgttcct tattgttgat gcgatgatag caaaatgcga tattactcaa
     7321 tactatggca tccgtcggat caacagtttc agctttaagc agtggcgtca aggcttcctc
     7381 atagttactc gccagcatat aactaatagc aagatttgac aagttttcga tattgtttgg
     7441 atcgtattgt aataccgctg ttgcgatttg tccaatttgc acgccaaggc tatctacgtt
     7501 agtatcatac aattgaacaa tatatgattg tatcgattca ttaaatgagg caattcctcc
     7561 atcaattgct aaaccatcgg tccatttcca gttatgatta aatgttgccc cgtaatggat
     7621 ggttttaact atttcatcag caaatgcctg ataagattga gtttctccca ataaatagat
     7681 tttaccaaat cgcaggtcga gcctatcggg gaataatgca atcgcagaat ctatgcatgc
     7741 aatagcagat gctacttgtt ttggctgata atagacatcg gcatacatga attgcggtgt
     7801 ggcatctaca gaggtatccg ttggtgttat ctccatgtaa ttattgccct tcggattgct
     7861 gcctatggta acaatactct tcatgctgct ccaaacaaga aaattgtacc tggcaacata
     7921 ccattccgga tcaccgggca catctttttt ccattgcgta aggagcacct gcgctctggc
     7981 agtatcgttt ttgtccagca actgattgaa ttgctgcgtg cgcgattgtg ccgatgccca
     8041 tgaagggagt aataacagca ataaggaaaa tacaataccg aaactgtagg tagaaagttt
     8101 atgcatgcct gtgaattaag taataaaccg cctttatatg caaaaagcgc gcctatttat
     8161 acaaaggtgc tgtaaaatct gcatttgagc aatgtctgag ttttgataga agtagtgccg
     8221 tttaccaacc taaaagtgtt tatcaacagt cgatttccat ggctaaagcc ctatctttgc
     8281 agcaaagttg aaattactat gtctaacgct atttttcgca tacctaaagc cctgaatgaa
     8341 ccggtaaaag gctacgctcc cggcagtcct gaacgcaaag ccttaaagca ggcaattgcg
     8401 gaccttaagg ctatttctgt agatataccc atgtttattg gcggaaagga agtgcgcacc
     8461 ggaaaaacgg tagcaattca tcctccgcat gaaattgccc atacgcttgg ccattaccat
     8521 cagggtgggg cagagcatgt aaaaatggct atcgacgcgg cattgacggc aaagcaagca
     8581 tgggaaaata cgccatggga agaccgtgca gccattttcc tgaaagctgc tgagttgctg
     8641 gcaggacctt atcgatacaa gatgaatgct gccaccatgc tcggacagag taagaatcca
     8701 tttcaggcgg agatcgattc tgcctgtgaa ctgattgatt tctggcgatt taatgccgag
     8761 ttcatgcagc aagtgtattc cgatcaaccg gaaagtgctc ccggtatctg gaatcgcatg
     8821 gaacataggg ctttggaagg atttgttttc gcactttcac cttttaactt cacttcaata
     8881 ggcggaaacc tgccaacagc gcctgccatg atgggcaata ccgttgtttg gaagacctcc
     8941 aatacgcaga tatattctgc ctgggtgatc atggaaatac ttcgtgaagc cggacttccc
     9001 gacggcgtta tcaaccttgt tcacgtaagc ggaagtgaag ccggaaaggt gatttttagc
     9061 catcctgact tcgccggtat ccactttaca ggcagtacgg aggttttccg caccatttgg
     9121 aagaccattg gcgacaatat tcatgtttac aaaacatatc cgcgcattgt gggcgaaacc
     9181 ggtggtaagg atttcgtaat cgcacatcca agcgcccatc caaaacaagt ggctacagcc
     9241 ttgagccgcg gtgcttttga gtttcaggga cagaaatgtt cagcagcatc tcgcgcttat
     9301 attccttcaa atatgtggga agaagtgaag cgttaccttg tcactgatct ttctgaaatg
     9361 aaaatgggcg ggattgagga cttttcgaac ttcataaatg ctgttatcga cgaaaaggca
     9421 tttgactcca taagcggtta tattgccaaa gcaaaagccg atggtcagga gataattgcc
     9481 ggcggaactt atgataaatc aaagggttat tttatagcac caaccgttat tttggctaaa
     9541 gacccgtcgt acagaaccat gtgcgaggag atcttcggac cggtgctgac aatacatgta
     9601 tacgatgccg aaaagtggtc ggaaaccttg caggtagtga ataacacttc accttatgcc
     9661 ctgacaggag ctattttttc acaagataga tatgctgtag tcgaggctac cgtcgctctt
     9721 cgccatgctg ccggaaactt ctacattaat gacaagccaa caggtgcagt tgtaggtcag
     9781 cagccatttg gcggtgcaag ggcttcggga accaacgata aggcaggaag catcctgaat
     9841 ctgttgcgat gggtgtcgcc aagaaccatc aaggagaact ttgttccacc ggtggatttc
     9901 cgttactcgt ttttaaatga agaataaatc tctattttta cctgatgatg cagcgctacg
     9961 cagggatcat aacaagcata ttgttttcca tattgggttc ttcctctatg gttcatgctc
    10021 aatcatatga tgcagcccct gatttttacg agatcaacga agatgttcct gatgttacca
    10081 tggacgttat ggcaaacgat ctcaatacta ccggtgtcac ggatgtatac ctgaacatag
    10141 caactttgcc attgcatgga accgttacgg ttgacgcagt aaacgacctg ctgatctatt
    10201 cacctaatga cgactactat ggcaccgaca atttcgaata taccggatgt gctaatacag
    10261 atcccgacat ttgtggaaca acaagtgtaa tcattactat caaccctgtt cccgataaac
    10321 caatcgctgt tgatgatgca gttgaaactt atgtaaatac accgctcaat atcgaaccat
    10381 tactaaatga tattgatgta gataacgaag gccttgaatt taccattatc gacgatgcag
    10441 attatggtgc aacaaccatc attttcggtg aagctgtttt ttatgaacct ttcccttgtg
    10501 tgtatggaga ggattgcttc atttacaaga tttgtaaaat aggttccgat atttattgcg
    10561 atacagcaac tgtatgtgta gatgtgcaaa ctacaaattt caatccacct tctgcattaa
    10621 atgacacagc aacagtagta gttatcgata ctattgatat acctgtttta ttaaacgatt
    10681 ttgatggtga tggcgatgct atgatcgttt cagagatcat tgctgacggc atgaccggaa
    10741 cagcaacaat taatgcagat aataccatca aatatattcc ttttgttgtt ggcaccgatg
    10801 atttcagata tgttgtttgc gacaataatt gtcctccata ctgcgatact gcatatgtgc
    10861 atatgaaaat tgaagagccg gaaattcccg cagaagcaat tcatgtgccg aactccttct
    10921 cgccaaatgg tgatggtatt aatgatatgc tcaccatcga aggacttgaa tcgggaatta
    10981 aatacaacgt gaatatatat gaccgcaaca gcagtcttgt atttgagaaa gaaaagcttg
    11041 ccacctcaga tgtatgggat ggcacgtcaa atgtggaatt tatttcgtca tcaggtaacg
    11101 ttcctgaggg cacttatttt tatgtgattc gtatagaagg actgcctgat ccattatccg
    11161 gctttattgt agtcaaacga tgaagaaatt ataccaaagc acactcctat tgctgatgtt
    11221 atgcagtgca aatgcattgt atgcgcagtt accgccttta tttgagcaat atcatttgaa
    11281 tcagttggta ttcaatcctg catatgcagg tagcaaaggc ggtatagagg ccggattctt
    11341 tttacaccga catagcacca atgttcaagg cgctccgggg accgaatcat tcacattgca
    11401 tactccgctc gcaaatgata atgttggact tggactgaaa atctatcacg ataaactagg
    11461 tgttactaat aatagttttg tgggacttga ttacgcctat cgcatgcata tcagcgatca
    11521 cctgacagca gctatcggga tagaaggttc aatcgctaac tacaaagttg attatagcca
    11581 gctagatgcc tttattaatg gcgaccccac ttttactaag gcttctgagt cttattggtc
    11641 gcctgatgcc ggtcttggag tttacctgca ttccgatatt tattatgtag gattatctgt
    11701 aatgtctcta tccggattaa gtgctaatgg aaatgacaac cctgatgtag ctcaagatga
    11761 aaaattcgac aaggttgcaa ctgtatatgg tacacttgga acactgataa agattaatga
    11821 taagcttacc ataaagccgg acttgctgat caaaattact gatagtattc ctgctcaaat
    11881 ggatgtgaat ttaaatttta tatataacaa cgttttattg gtaggtggtg gttaccgaac
    11941 aaatggaagt atctcatttg tgggtcaata tattttcagc acagataata agatcaccgg
    12001 acatgaagcc ggcttaggat attcttacaa tacgatgttg gcagatgaca aggtatttat
    12061 cagcccatct catgaggtgt tcctgaccta tcgtttttat aagcataaca ataagtttgt
    12121 taatccaaga tttttctgat gcgcatgcaa gctatatatc gttactgtct gatcataatc
    12181 cttttttcag gaatcagccc actgcacgca cagaatgaaa ttctggcaaa agcggataaa
    12241 tattacaata atggaattta tccggaagcg gcagcactct atatcgacca tctggagcag
    12301 caatatgatt atatcgtcaa tgtgaaattg gcagattgct attatcgaat ggaggaatgg
    12361 agcgaagccg agcattggta tggcgttatc gtatcacata atatagctga tgccggtaca
    12421 ttacttagat atgctacgct gcttaaaatg aacggcaaat acaggtcggc aaaaacatat
    12481 tttttaaaat atgcggctta taaacctgat ggatattatc tcgcatcaac atgcgattgg
    12541 gcattggcaa atatgaataa gccatcgcaa tattatatcg atacattgcc atttaatacc
    12601 aaaggctcgg aaatgacacc gaccatttat aagaacggaa ttatttttgc taagagtaat
    12661 ggcacaaaaa tcaataacaa tacaggtctg ccttattatg atttatattc agccgatgtg
    12721 cgaacagacg gaagctggat tacaaataaa ctcgatcatg tgaactcacc ttatcatgat
    12781 gctgcacctt attacgatgg tgcttctaaa ggtttgtatt ttacgcgcag caaccattat
    12841 aaaaacagaa cagtggtgag catcgatggg caggttagac tggagttatt ttatgcacct
    12901 tatgtagaca gtaaattcgg cacgcctcat ccgttgaacg tcaattcaaa aatatattca
    12961 gtaggtcatc cggcaacaac tcccgatggc aacattttaa tttttgcatc agacaagcaa
    13021 ggcggaaaag gaggaacgga tctttattat tgtatgaagc gcaatggcaa ttggtcagaa
    13081 cccaaaagca tgggtgatgt gattaatact gccggagatg aattgtaccc cttcatgaca
    13141 gccgatggca ctttgtattt tgcgagtaat tatcatccgg gatttggcgg attcgattta
    13201 tttaaatgcg aacgcgaagg tgatcattgg tcaactcccg aaaacctcgg catgccggtt
    13261 aattcacctc aggatgatta tggctttacc atgaaaaatg gcgttggtta ttttagttcc
    13321 aacagacccg gtggcatggg cggcgatgat atttatcaag taacccaaat ggctcagatc
    13381 gcttcagtat ttgtttacga caataataaa aatgccattt ataaagcgcg cgttaccttc
    13441 tttgaaagtc ctaattcaca agtaatctgt gaaacagatc caaacggata tggcgatgtg
    13501 agcacgcttt ccggagtatc tacctctatc aaaatttcga aggaagggta tttggataaa
    13561 gtgattaaca acttgggtgg tctgcgctcc agcaacggtg tactccctgt tgaactcaca
    13621 ccgctcatcg gaggcaacga ataattcgtg ttaccaacct atttgcagta attttgttgt
    13681 cggttattat tttccgtcaa cacaatagta tcatgcagca aaacaacaat aagccgatta
    13741 ttctcatcac taacgatgat ggcatacatg ctcccggtat acatgcgctg gtagaagcaa
    13801 tgcgtccact tggaaccatt gttgttgttg cgccagataa gcctcaaagc ggtatgggac
    13861 atgccatcac cctcaatgag ccattgcgat tgaaaaaatc aaatcttttc ggtgatatcg
    13921 aagcgtatgc atgcacagga acaccggtag attgtgttaa gctggcgaag gataaaatac
    13981 tccataaaaa accacagctc tgcgtttccg gaatcaatca tggaagtaat tcttcaataa
    14041 atattattta ttccggcact atgtccgcag ctatggaagc cggaatagaa gggattcctg
    14101 cagttggatt ttctttattg aattatgcaa ttgatgcaga ttttacctca gcggcgcact
    14161 acgcagcaaa aattgcagaa caagtattgc aacatggact gccacaaaac acgttactca
    14221 atgtaaatat tccatatctg cccatcgacg aaataaatgg catgcgcatt tgcaggcagg
    14281 cacatgcgaa atgggaggaa gaatacgatg aacgcctcga tccgcatggt cgaccttatt
    14341 actggctaac aggtaatttt gtgaattatg atggtggcga tgacaccgat gaatgggcat
    14401 tgtcgcatgg ctatgtgtcc atcgtacccg tgcaatatga ccttactgca caccaggcga
    14461 ttgcgttttt tcataagaac tggtcactct atcaaacaaa atcacctcac gatatacact
    14521 tagataactc tgatacgttt aatgacctcg gataattgat atgagcaaac tacaggaaga
    14581 taacgttttc gtaggaatgg ctgttgctgt tttttttatg gtgacaactt attatgcctt
    14641 ttttcaactg aatgagagtc tatccggaaa tattaccatt aagggtgcaa tatttaccgg
    14701 agtgactgaa agattcattg caacgctggc tgtctttttt aatattttac cttttgtcat
    14761 ttatatgcgt gttcgaaagg ataattgtat gcgaggtgtt ggtatcatta cagttttgag
    14821 tgcaatgttc atcctggtgt atttttatat tcagcatcat tcaacaattt ttcaataacc
    14881 ttcatcttgc gctactatct catagccggc gaagcttctg gcgacctaca cggcgctaat
    14941 ttggttcgcg cattatcggc caaggatcct gatgcagttt tccgcggatt tggcggtgat
    15001 gctatgcacg aagcaggagt ggatattgta acacattata aggaaactgc ttacatggga
    15061 tttgttgaag tgtttatgca cctcagaaaa attctgaata atattaaatc atgtaaacag
    15121 gatataaccg ggtggaaacc tgatgtagtg attctaatcg attatccggg atttaatttg
    15181 aggatagcac cccatgtaca tgaaattgga attaaagtgt tttattacat ttcaccgcag
    15241 ctttgggcgt ggaaaagtag tagggtgaat attattaaac aatatgtaga tcgacttttt
    15301 gttattcttc cgtttgaaga agcattttat caaaaattta attatccggt ttcctttgtc
    15361 ggccatcctc tgctcgatgc tattgcttca aacaaaggca atgaacattt tcgtgaacag
    15421 tttcagttag gcgataaaca attgattgct gtattgcccg gaagcagaaa gcaagagatt
    15481 acagttaaat tgccggttat gctcagtatg gcaaagtatt atccggaatt tgtttttgtg
    15541 gttgcctgcg ctccgggaat ggacgatagt ttttatgccg gcttcgaaaa acgggagaat
    15601 atcatttaca tttcaggtag tacttatgat attttgcgaa atgcttatgc agctatggta
    15661 acttcaggca cggcaacttt agagactgca cttttccggg tgcctcaagt tgtttgttac
    15721 aaggggagcg tcatatcata tcatattgca aagcgcctgg tgaaaattaa atatatcagt
    15781 ctggttaatt tgatagctga taaacaagtg gtgcctgaat taattcaaca tgatttcaat
    15841 cacgattcac tgcgcactca tctcgatctc ttacttaagg atgcggcata tcgcaatact
    15901 atgttgagcg aatatgataa attatatacc gtattgggtg gagtaggagc aagcgcaaga
    15961 actgccgatg aaatgatgaa gcttttagcc gaagggaaat aaaaaaaggt gccacaaagg
    16021 acaccttaaa gtggagatag tgagactcga actcacgacc tcttgcatgc catgcaagcg
    16081 ctctagccaa ctgagctata cccccggaag cggggtcaaa gataataatt ttcagcatct
    16141 ttgttaacat atgacccatg ctccggtaac tacgatttac atgcatgacc atccggttga
    16201 tattctgaga ttggagctgg cacaaccgcg tttacaggga aataagttct ataagcttaa
    16261 gtataatata gagcgcttta aacaagaaaa ctgcgacact ttgctaacct ttggcggggt
    16321 gttttcaaat catatccatg ctgcagcaac tgcctgttat gaccacggaa ttcgaggagt
    16381 tgcagtgctt cgtggtgagg atgatccatt caatccaaca ttaaaattta ttcgctccat
    16441 gggcatggag accatcttcg tttctcgaga actttaccgc caaagaaaca atagtgattt
    16501 tttacatcac ttttcaattg atgcaaataa aatatatgtc ctgcctgaag gaggcaccaa
    16561 tgaatttgcc gtcagaggct gcagtgaaat acttggtgat cttgcagata aatatgacag
    16621 aatattttgt gctgtaggca ccggcggaac actatgcgga ctcatttgta gtcccggact
    16681 tagtgcaaca gttaccggta taagctcatt aaaggccgac aaagattcat taacaccata
    16741 tatcaggcaa ctttgcggtg atgcgggcaa taaaacgcaa tgggatatta atttccaata
    16801 tcatttcggc ggatatgcaa aatacaatga taccttaatg gcatttgttg agcaattcga
    16861 acacaaatac aatattaagc ccgatcctgt ttatactgcg aaagtgtttt atgctgtaaa
    16921 tgatatgatt tcaaataaaa gcatttccgc ctccgaacgc atattgattt tacatacagg
    16981 tggattgcaa ggttgggatg gatggcatta tagatttcat cacccgaaaa agcagtccca
    17041 tgcataatca atcgttttgg aattcaccga agcggatacg cggtgttttt atcatcgcgc
    17101 acttgacttt attttgcttc ttcatgtggc gatttatcat ttcaccaaac gatgttatgg
    17161 tcagcggcag gaatgatggg ttaaagaatt tcttcacttt acaagcttac cttgagcaac
    17221 ctgcttctga tggctatatt gattttacag caatgcaata tccttatggt gatgctgtgt
    17281 ggtataccga taattcgcct ttcattgcta tgccattgcg atttattcaa ttgcattttg
    17341 gcaatatcgc accatatact gtagcaatta tccaatggat ttttttattg ggaatattgc
    17401 ttactccttt aaccgttatt cccttactgc gtcgatttct aacgaatgaa tggttaattg
    17461 caataggtgc tattgtaatc acctggacca gccctcaatt tttgagattt tttgttggga
    17521 attataacct ttcacttact ccggtttatt tccttgtcat ggcgttatta ttgcggattt
    17581 acgataattt ccaaagtcgc aatcgaaaaa aactgctctt acatgcagct ttgatcgcca
    17641 tcgtgatttt tcttgcttca gggatacatt tatattatgt catcttactc gggttacctg
    17701 caattgcttt tttgggaata ctattccttc agcaaataag aaagtctgcg gaagtgctgt
    17761 attttactgc aattccgagt tttgccatag cgattgcaac ttttggtatg atgttattga
    17821 tcaataccac tgatccatgg cgctcaatgc gacctactca attggatggc agcaatatca
    17881 gtgcatggga gatgcggttt atgcatttgt ataaagccag agatgaagtg aacctagttc
    17941 cctttatagg aggtaaaatt catttcgacc cggaaaatgg agtttatttg ggcggattct
    18001 tctggtattt attgttgatg gtatttattt taattgcatt tcgttttttc cggtataggg
    18061 atgtctcatt tattaaaacg ctgaaacctt caccggtcgg atggattttt atacttactg
    18121 ccattgtggc attttttgcc ggaaccggca tgcacctgca tttgattggg cgtggaaaca
    18181 ccatggagaa tattttcaac cccttatatt atatcggaaa aatttatccg ggcgttagcc
    18241 agttccgatg tattgccaga atgggatggt ggttgttcta tgcggctcaa attggtattt
    18301 tggtcgccgt tgaacgaata atccttaatc aaaagaaatg gatagtgaat gcagtattgt
    18361 gtatcggatt atccctgctt gcaacagata tttatgggat aaagcaattt actaattcaa
    18421 ggttttaccc caattacttt aatcaaaaat ctctcgcatc acttccagat ctggattatg
    18481 acgcttacca ggcaattttg ccaattccat actatcatgt tggctgtgaa gaatatgatt
    18541 tcactatcga cgatgccaat gaatggtccc ggtatactta ccaattgcag ttgaagagcc
    18601 acttgccgct catgtcggtt aaattatcca gaacacctca gctttttgct tcccagatga
    18661 tgcaattact gcttgagcct caaagtccag gcactttgca tgaatattta aacgataagc
    18721 ctattcttgt gatctatgac ccatccatag aagaaaaaca gaatcttgaa ccggctgcaa
    18781 ccgttttgaa ggaatcaccc ggtctgataa catctgagca tatggtgctt atcggcagta
    18841 aaggcaatgt gcaatattat gcatggtatc ttaagtcaaa ttgaagtaat tttgaatcat
    18901 taaaactatt atatggattt tattaataat tatattgaaa caaataaaga aagactcctt
    18961 gatgagctct ttgctttatt gcgcattccc agcgttagcg ctgatccgaa atataaagct
    19021 gatgttgccc gcactgctca tgcagttgca gattcattgc gcgctgcggg tgccgacaat
    19081 gtgcgtgtaa atgctactga aggacatccg atagttactg ctgagaaaat tatcaaccct
    19141 tcattaccaa cagttttatg ttacggtcac tacgatgtgc aacctgcaga tcctgttgat
    19201 ctttgggata gtccgccatt tgaacctgtc attaaaaatg gaaatattta tgcacgcggt
    19261 gcttgtgatg ataaaggcca agtgtatatg catgtgaaag catttgaagc tatgatgcaa
    19321 aacaatgcgc tgccatgtaa tgtgaaattt attattgaag gtgaggaaga agttggctcc
    19381 ccgaatcttg gaactttcat aaaggaaaat aaagatttat tgagttgtga tgtaatttta
    19441 atttcagata cttccatcat cgccaatgac gtgccttcag ttacagttgg gttacgaggt
    19501 cttacttata tggaagttga agttaccgga ccgaatcgcg atttgcattc cggagtatat
    19561 ggcggtgcag tagctaaccc aatcaataca ctttgtgata tgatatcttc attgatagat
    19621 aaagatggtc atatcaccat tgaaggattt tatgatgatg tggctttgat ttcagatagc
    19681 gaaagagctg aaatggcaaa aacaccgttt agtctcgatg agtacaaaca ggatcttgcc
    19741 attaatgatg tcaaaggtga aaaggggtat tcaacattag agcgcagttc tatccgtccg
    19801 accttagatg taaacggaat ctggggcggt tataccggtg aaggaagtaa gaccgttctt
    19861 ccgtcaaaag cctatgcaaa aatttctatg cgcctggttc ctgatcagtc atctgaaaaa
    19921 attgcagaac tatttgacaa gcatttcaat aaaatagctc cgccatatgt gaaggtgaaa
    19981 gtgacccatc accacggcgg agaacctgta gttgtgccgc tggattttcc tcctatgcaa
    20041 gcagcaataa tggcaatgga aaaaactttc ggcaaaaaac ctattcctac gcgtgaaggt
    20101 ggttctattc caattgttgc catgttcgaa aaagtattgg gcgcaaaatc catactcatg
    20161 ggatttggat tgaacagcga tgccatacat agcccgaatg aacattatgg cttgttcaat
    20221 tattacaaag gaattgaaac attgccatgg ttctttaaat attatagtga actgaaataa
    20281 tgctgaaata acataaaagg cctccgagat atgcatgtcg gaggcctttt ttaattttgt
    20341 gaaagtcgtt ttaggaaaca agtatttcac ctgtcatttc ctttggaatg tcaatgccca
    20401 ttaaggttag aattgttgga gcaatatccc cgagtttgcc gtttctaatt tgttgaaacg
    20461 ccggctcctt atcgatataa aacaccggaa caggattagt gctgtgctgt gtattcgggg
    20521 tgccatcttc attaatctct acatctgaat ttccatggtc tgcggttaga ataacggcat
    20581 aattttttga taatgcagct tcaattacct cgcccacgca ggtatcaaca gcttcacagg
    20641 ctttaatggc agcggaaaac acgcctgtat gtccaaccat atcggcattc gcaaaattca
    20701 aacaaataaa atcagggctt tcgttattaa tagcttcaac aatggcatct ttaaccaagt
    20761 aagcactcat ctcaggttgt agatcgtaag tcgcaacttt tggtgaagcg atcataatgc
    20821 gtcgctcacc gctaaattcc ttttctctgc caccactaaa gaaaaaagtt acatgcggat
    20881 atttttctgt ttcagcaata cgtatttgcg ttttattgtt cgcttcaaga atttctccta
    20941 atgttttgct gagatcatct gattcgaaaa taacaccaac atttttaaat gtatgatcgt
    21001 aacgtgtcat ggtgacataa tgcagatcaa ttggtgtcat atcgaattcg ggaaacgctt
    21061 cttgtgttaa tgctcttgta atttgtcggc aacgatcggt tctgaaatta aaacaaataa
    21121 ccacatcgcc ggcggctatt gtagctaatg gtttttgttg tgcgtcagtg caaataatag
    21181 gtaaaatgaa ttcgtcagtt acattatcgg aataactctg ttcgatactt tttataacat
    21241 cctgcgtagc attgccttcg ccatgcacta aaagatcata tgctttttta atgcgttccc
    21301 atcgtttatc acgatccatg gcataatatc gtccacatac tgatgctatg gtgcctgctg
    21361 aatttgttaa atgttgttgc agtgttctga taaaacctac cccgctttta gggtcacaat
    21421 ctcgaccgtc ggtaaatgcg tgaatgaaaa cttggtcgga agtgagtcct tttgattttg
    21481 caatatcaca aagcgccatc aggtggttgc tatgagaatg tacaccgcca tcgctgacaa
    21541 gtccaatgaa atgtactttc ttaccgttgt tttttgcaaa atccagtgcg gataataagg
    21601 tattgtttga agccagttca ccgttgcgga tggccatatg aatacgctgt aattcctgat
    21661 aaacaatgcg acctgcacca aggttcaggt gaccaacttc actatttccc atttggcctt
    21721 ccggtaaacc cacaaactcg ccgtcggttc gaagttctgc atttgggtat cgtttataat
    21781 atgcgtttac atttggtgta ttagcctggg caatggcact agcggctgca ttttgaccat
    21841 gtccccagcc gtccataatg atcagcatca cctttttcat cgatttcatg atgcaaaggt
    21901 atatactggg cttggtttat agcatgggaa aggtcactgc cgataatgct cctaaaacct
    21961 gaacttctgg cggtttggtg caacttccag ggtaaggaac tgttatcggg cagttattga
    22021 aagctgaaac ccgcacctgg gttgatttta gccatgtcat ccacagacgt tgaaaggtat
    22081 cttgttaaaa aatgctaatt ttggtatggt tttagcattt accattgcat tgaccaaaaa
    22141 tacagccatg ttcaagaaca acagaacaaa gcttatagcc atccttgcgg tggttccatt
    22201 cctgattttc gctactatgc agagcgagct tgcgggaatt acttctgcaa ttaaatcagg
    22261 tgatagcaag tcgcttgcta cttactttga taatactgtc gaggttaaaa tagcgaataa
    22321 ggaaggcgct tacagtaaaa gccaggccga agcggtggta aaagactttt tcactaaaaa
    22381 tccacctaag gatttcagct ttttgcatga aggtacttca ggaggcagca acggccatta
    22441 tgctattggc tctctgtcta ccgataaggg taaattcaga acctacgtgt atatgaaaaa
    22501 gcgtgctgat aagttctata tccaggagtt aagctttgaa aatgagtagt gttgagattt
    22561 gggatattat agattttata gatgaggccc taattgaaga catccaggag ggagatcata
    22621 caaccctcgc caccatagat catcaggctg ccggaacggc aaaattgctt gtaaaggaac
    22681 cggggatcat cgccggtgta gaattagctg aacagatact tagacatgtc aaccccgaat
    22741 tggaggttga cattttcatt ccggacggag cacgcgttga accgggtgat gttgtttttc
    22801 atgcaacagg aagagtccag gccttgttgg ttgccgaacg taccatgctc aatttcatgc
    22861 agcgcatgag tggaatagca actaccacaa atctctttgt tcaaaccgtt agacataccg
    22921 gcgccaaaat tttagatacc agaaaaacaa cgcccggatt gcgcacattc gagaagtggg
    22981 ctgtgcgaat tggcggtggt cataatcata ggtacggttt atatgacatg atccttataa
    23041 aggataacca catcgatttt tgtggcggga taacacctgc tattctgaag gttaaagaat
    23101 acctgcgcga caacgattta aacctccgca ttgaagttga aacccgcaat cttgaagagg
    23161 ttgctgaggt tttgcgaaca ggtcatgtcg acagaattat gttcgataat tttgatgtag
    23221 acactatgcg tcaggcggtt attgaggtag ccggtcagtt cgaaactgag gcatcgggag
    23281 gtgtgaattt gacaactgtt caggctatag ctgaaacagg cgtaaatttt atttcagtag
    23341 gcgccttaac acactctgtt aaaagccttg atctgagttt taaagcgctc attcactaag
    23401 gcgcttccaa cataatcggt aatttgtttt agtaactttg caagctgtat gaaagaacaa
    23461 ctgttccata ttgagatatt gctcaaacgc ttgggcattc tcctgctgtt gtatatggtt
    23521 tgccgtatac tgttctttct gtttaatatg gcacttttcc cgcacatacc tccgggagag
    23581 tttttgcagc tgatgttgta tggtctccgt ttcgacttgt cgagtatcat ttatgtgaac
    23641 tgcatattta tagtattgca tgtagttcca agtccatggt tcgataaaaa ttggtatcag
    23701 cgcatgttga agatcatttt ttattctttt aatggcgttg ggttattatt ggagagtggc
    23761 gattggatct attatgaata tggattaaaa agaacatcaa cgcacgaact cggacttaca
    23821 ggtgatacaa atattcttcc gcaggtaatt aaagattact ggataatttt tgtcattgcc
    23881 tttatactga ttttaggagt tgaatatctc taccgaaaaa cagaattgca tcgacttaaa
    23941 aaagtgcatt tcaaactacc acgttttatt cattatccat atcagatcat catcatggtg
    24001 gtggtgctta cattttctgt gattggagca agaggtggta tacaaccgga acctatttca
    24061 ccggaaattg caacagatta tgttgatgat ggaagactca catctttgat tatcaatacg
    24121 ccattctctg taatttacgc gttcggccac aggcaactag ttgagcccgt ttattatgag
    24181 gatgaacagg aattggcaaa gcagtttaat atttttcacg ataacgcact ttattatcag
    24241 tggcctgatt caattgccaa gccatctaaa cctgaaaatg tatgcgttat tgtattagaa
    24301 agcttttcaa aggaatatat cggatacttt aatcacggcc atgggtatac accatttctg
    24361 gattctttat taacggtagg tatggcattc gacaatgctt atagcaatgg gaaatcgtca
    24421 aatcaaggga ttgttgccgt tacaagtgga atccctgttt taatggaaga gccctttatt
    24481 tcatccattt atagaaataa taaatttgaa ggtgttggta ccttattgaa agatgtcggc
    24541 tattcatcct atttcttcca tggagcgaat aatggttcca tgcagttcga caggtttatg
    24601 gctcaggccg gatttgacgg atatttcgga agaaatgaat atggtaatga tgcagatttt
    24661 gatggtaact ggggaatatt tgatgagccg tttttgaaat ggactgccaa taaaatgaca
    24721 accttaaaag cgccatttta ttctgaaata tttacaattt catcgcatca cccattcaca
    24781 gtaccggcac agcatgtagg tcagtttccc aaaggtccta ttccgatgtt ggaggttgtt
    24841 ggctatgctg attatgcact tcagcagttt ttcgaggaag cgaaaaagca accttggtat
    24901 aacaacacat tatttatcat cactgccgat catcccggac ctccgttgcc tgaacatgaa
    24961 ttttatcagg agcaagttgg ggcacattct acgtggttgt tgatgtacaa acccaacggg
    25021 caatttaaag gagtgaattc tgaggtggtt cagcaatctg atatcatgcc tacggtttta
    25081 gattatgttg gctataaagg aaaatatatg gcctttggta attctatttt tgatacaacc
    25141 gcagcgcgat atgcatacaa ttatcatacc tcggattata tgttactgga taaagatttt
    25201 gctttgatat tcaatggcga tcgcccggtg ggtttgtatg attataagat agatagcact
    25261 ttgagtaata atcagatgca tgagtttcca gacattactg cgagcatgga agatcgaata
    25321 aaggctatca ttcagataca ccaccatgct atgatcaaca acaaactggt ggcagagtga
    25381 aaaagcacat tctgtttatt attaacccgg ttgctggggt caaacgcaag gatcgtatcc
    25441 ctcgtttgat agataaatat ttagaccatt cacattttga ttatgaagta atctataccg
    25501 aacgtcgggg tcacgctaca caaattgctt ctgaagctgc agcacgcggt gtgcatgtgg
    25561 ttgccgttgc aggtggagat ggaagtgtaa atgaggcagc aaccggtttg atcggcacga
    25621 atacttgttt ggcaattatc cctagcggat ccggtaatgg tcttgccaga catctgggat
    25681 actcaattaa tattcgctct actataaata ttattaatgc ctatcatgtt cgtaaaatag
    25741 atgtgtgtaa ggtaaatggc accatgtttt tcagcttgat aggtattggt tttgatgcat
    25801 ttgttgctaa agtgtttagc cgtgaggaaa cacgtggatt tctgacttac gcttggagtg
    25861 caattaaaag tgtttcagca tttgatgctt ttgaatatag tgccaatctt gccgatagga
    25921 aaattggtgg caaagcgttt atgattaatg tgtgcaattc aaatcaatac ggttataatg
    25981 taaaagtggc gccacatgcc agtttaagcg atggtatgct tgatgtaatc gtactgggtg
    26041 atattccgaa atggaaaatt ccattggtgg tattgcaggt attcaccaga acgcatatgc
    26101 atactaagca ttttactatt tataaaacac cttccttaac aatagatacg cctcaatatg
    26161 ctcacttgca aattgacgga gaaacagcac caaaggataa atcttttaaa attgagatta
    26221 tgccggaagc actacaagta ttgatgcacc ctgataacga ttaatgtatg gcaaaacaaa
    26281 aattgaatct gtcgggactg gtttattcta caaatcctga ttataaaccg gaatcatccg
    26341 aagaaaatca ggaaaccctt gcacctgcac aacaaaatct tcgcgtatgg ctggagaaga
    26401 atcatagagg tggaaagact gctacggtaa ttagggattt tgtagggaat accgatgacc
    26461 tcgaggcttt gtgtaagatg ttaaagtcga aatgtgggac cggtggaagt gcgaaggatg
    26521 gagagattat tatccaggga gatcatcgaa aaaaaattgg agaaatttta ggcggcttgg
    26581 gatataaata taaactcgca ggcggttaat cgattaggct atgcgaaggc cattctttac
    26641 ggttctttct gtttgtaaca atactacatt tccatcagcc tcaattgcgc ctaagacgag
    26701 acattcgctg aaaaaatttg ctatttgctt tacggggaaa tttacgactg caactacctg
    26761 tttgccaata agcgattctg cagtatagtg ttttgttatt tgtgcgctgc tgcgttttat
    26821 tcccaaagca ccaaaatcga tggtaagctg atatgcgggt tttcttgcag cgggaaaagg
    26881 ttcggctgaa atgatagtgc cggttcttat ttcgattttt tcaaagtctg aataggtagc
    26941 catagcatgt ttggattgat ggaaatcaat tacaggtttg aacaataata ttaaatttct
    27001 ttaagcctta cgggcgcgag gtcataatcg ccggtatata cattcttgtg aaccatttct
    27061 ccggtttgca cattaaaaat atttacaaag gattgcgtat aatgcatatt atagattaat
    27121 acaaaattta tctctttgcg aagtatcagg tcgctgagtg agtcggaatc gacgaatgtt
    27181 agcttgccaa agtatttatc tctgaattca gattcttcat aatattcatt tgcacgtggg
    27241 tcaattaatt tgaatttttt taggcagtaa tcaggtatgt aaagaacgtt tttgaacaag
    27301 gctgttttgt tgctgttcaa ggtttctgtt ttttggaaat gtttgacaga ggttcctgtc
    27361 tctagattct tttgtatata ttgaaagtac aattgaagaa aacccggtaa atagttgtga
    27421 atttgaataa cagaatcctc cttagtaatg atgtctttat aagttgtatt aaagtattta
    27481 taatctgcat acataaaaat ctctgccagc ttgatatttg tggggccctt tttagttact
    27541 gaatctttaa acatcatggt taatacagca gtggacatat cggcctttcc agcaacggct
    27601 gcccgtgcat ccaacgttac aaacgtatag ttttcactgt cctcataata taaaagttca
    27661 tcacttgtta ctacctcaac ttcacttaat gtccagccgt tgcgcagcgc tgtttcgtat
    27721 ttggggattt gctcaatttc cgaatcgcgt ataacaaata tgagattgcg ttttttaagc
    27781 tcagaaaggt tgtgtgccat ttctttcttg gagtattcga aaacagtaat ctgtgcctgt
    27841 gaagctcgca ttgatgcgaa taggatcagt actagagcaa acaaatgggt agttttcatg
    27901 cttttaactt tataatctgc acgaaattta gcaatagttt gtctgatcaa gaaataatat
    27961 attatttggt ataagttgga agagtaatct agatttctat tcctaagttt ttaggtatac
    28021 atagaggtgt aaaccgtttg ccaagttggt tggcgcaatc ggaattataa tcctgattgc
    28081 gatttattaa gtacagtttg tcaacaaaat acgactttat aatttaacaa atgtttcgaa
    28141 catgcgctcg tttgattgaa tggcacaaag cagatacatg ccggatggta aatctgaaat
    28201 attaattgtg tatgcacatt ctccatacag tgccggaact ccaattacgt tttctaagtt
    28261 ttttccattt aaatcataaa tacaaaaggt tggtatgtct tcggccagtt ggttaattgt
    28321 aacagtaata tctgtatttg cagggtttgg aataatgctc attgatgaag aggtaattgt
    28381 attagggaaa gcttcttggg tatagaatgc caaaccacca taagttccca cccatacagt
    28441 attgttttca tctatataca aggcatcgca aacattgttt ggcaggttcg aattataatc
    28501 atagtacaaa gtaaagtgat tggtgctgtc ggcacgtgtt acaccaccat gagtggccat
    28561 ccagaaattg tcatcatcat caaaccccat tctgtttacc ttttctgatg tcatgggtac
    28621 agtaaataaa ttatatacaa acaaccaatt atcggcaact atatcatatc tgttgacacc
    28681 aagataatct gctacatata acaagtcatc ctcatcttga gtaatagccc gaatggcatt
    28741 gttatttata cctgaattcc cataattaaa ggtttcccag gtgccattgt catatttaga
    28801 taagcctgca tattttgtcc caatccaaat gataccctct ctactttgat ataggcatgt
    28861 aagcgcattg cctgccaaag gtgaagcaat agaatcgtag tttgtccatg ttccttcctt
    28921 taggattgac agtcctttat cggttgcaaa ccaaatgctg ctatctgctt ccacatacat
    28981 aatgtctttt atgctgtttc cgataatgtc tgaatttgtg gtatcgaagg atgaccagcc
    29041 ttcatcatgt aaaatgaaaa gaccataatt gtttgatccg gcatatacat cgccattgac
    29101 acatagaata cttcgaaaat cattgtccgg gaagcctgac tttgcactgt tgtaggtagt
    29161 aattgcccct gtagcgggat tgaagctctt gactccatcg gcctcagttg ccgcccagat
    29221 aagggttcca tcaccatgaa tggccaagat ggtgtcggta atacctgtcc ggatccaggt
    29281 ttgctgcgca cggagacttg tcacgcaaac gagtattgaa atcagcccaa aggttttcat
    29341 ctgtaggagt gtgaacgatg caaaaatcgc attattgtgt gaaacaatgc aattctagac
    29401 gaaggttaaa gggaatcggg agcggtattc ggcaagtgtt tgaaa
//
LOCUS       DAOQOU010000021        29188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_719_length_29188_cov_6.250712, whole genome shotgun sequence.
ACCESSION   DAOQOU010000021 DAOQOU010000000
VERSION     DAOQOU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 29188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29188
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_719_length_29188_cov_6.250712"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLM90_03310"
     CDS             complement(<1..887)
                     /locus_tag="PLM90_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ88426.1"
                     /translation="MPKVTIDDITIEVPAGTTILEAARQIGVNVPPAMCYYSKLKGSG
                     GKCRVCLVKVTKGSDKDPRPMPKLVASCRTTVMDGMEVRNVTSPEVIEARRGVVEFLL
                     VNHPLDCPICDQAGECHLQDLSYDHGLSKSRYDFERRTFERIDIGDKIQLHMNRCILC
                     YRCTQVAEQLTPERKHGVLYRGEVSEISTFIATAVDNEFSGNMIDVCPVGALTDKTFR
                     FKSRVWFTKPMYAHRNCPTCSGKVALWYQGEEVLRVTSRKDKWGESEGFICNECRFDK
                     KNTSDWTIEGPAPVDRHSV"
     gene            complement(952..2298)
                     /gene="nuoF"
                     /locus_tag="PLM90_03315"
     CDS             complement(952..2298)
                     /gene="nuoF"
                     /locus_tag="PLM90_03315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMZ88427.1"
                     /translation="MGRKLLLENLDKPEINTIDGYIKYGGYRALEKALKSMTPDEVTE
                     EVKKSGLRGRGGAGFPTGMKWSFLDKSTDKPRYLLCNADESEPGTFKDRLLMEKIPHL
                     LVEGMIVSSYALGCRTSYIYIRGEYMFILRILEKAIAEAYAKGYLGQNILGTGYSLDL
                     HVHPGAGAYICGEETALIESLEGKRGNPRIKPPFPAIAGLYNCPTIVNNVESIAAVVP
                     IVNNGGEDYAKIGIGKSTGTKLISASGNINKPGVYEIELGVSVEEFIYSDEYCGGIAN
                     GLDIKACVPGGSSVPILPKDLLFKQSDGTPRLMTYESLSEGGFATGSMLGSGGFIVFD
                     EKACIVRNTWNFARFYHHESCGQCSPCREGTGWMERVLHNIEYGHGKMEDIDLLWDIQ
                     RKIEGNTICPLGDAAAWPVAAAIRHFRHEFEYHVKYPHRVFDADHVYQPEMKMQTV"
     gene            complement(2334..2840)
                     /gene="nuoE"
                     /locus_tag="PLM90_03320"
     CDS             complement(2334..2840)
                     /gene="nuoE"
                     /locus_tag="PLM90_03320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMZ88428.1"
                     /translation="MTEAKHIELSPEAMMEVGRIISHYPADKQKSALLPVLHIAQKEF
                     GGWLSSESMDYVARLLHIHPIEVYEVATFYSMFNTKPVGKVLIEVCQTGPCCLNGAEK
                     IVSYLEKKLQIKVGETTRDGMFTLKTVECLGACGYAPMFQIGETFYEHLTEEKVDNIL
                     LEMRNKNS"
     gene            complement(3125..4342)
                     /locus_tag="PLM90_03325"
     CDS             complement(3125..4342)
                     /locus_tag="PLM90_03325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMZ88429.1"
                     /translation="MAKNENIINGVVQTEEELKQYNYLNLGPTHPATHGIFQNILKMD
                     GEIIVDAQPTIGYIHRAFEKLAEHRPYYQVTPITDRLNYCSSPINNIGWHMTVEKLIK
                     AEIPKRVDYMRVIIMELARIADHIICNSVIAVDTGALTGFLYVFQWREKIYEIYELIC
                     GTRLTTNIGRIGGFERDFSDDVWRRIQEFVDEFPAALKEFEKMVMRNRIFMDRTIKVG
                     PISGERALAYGFTGPNLRAAGVDYDVRVMSPYSSYQDFDFIIPVGTNGDTYDRFCVRE
                     EEMWQSLHIIKQAMQKMPSGDFKGDVPEFYLPPKQDVYNTMEGLIWHFKIVMGEVHVP
                     KGQVYHSVEGGNGELGFYLVSDGGRQPYRLHFRRPCYIYYQAYPEMIKGAMLSDAIIT
                     MSSMNVIAGELDA"
     gene            complement(4577..5086)
                     /locus_tag="PLM90_03330"
     CDS             complement(4577..5086)
                     /locus_tag="PLM90_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMZ88430.1"
                     /translation="MENKIENHLVESLLREQFGDAIKASEESYGMLDITVSRDKIVEI
                     LHWLRDHSILQFNFLTSLCGMHFPEVKNAELSVVYHLHSFINNVRIRLHIFFPANDAN
                     APSVIELFPTANWMEREAFDFFGIRFTGHPDLRRILNVEDMQYHPLLKQYPLEDGTRT
                     DKEDKYFGR"
     gene            complement(5086..5628)
                     /locus_tag="PLM90_03335"
     CDS             complement(5086..5628)
                     /locus_tag="PLM90_03335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HMZ88431.1"
                     /translation="MALQKVNEIPEMYTGEGYALTKLEKVVGLARANSIWPLPFATSC
                     CGIEFMATMAATYDLGRFGAERLSFSPRQADLLMVMGTISKKMGPVLKQVYTQMAEPR
                     WVIAVGACASSGGIFDTYSVLQGIDKVIPVDVYVPGCPPPPEAILDGLIKIQELVKIE
                     GFRRRDSPEYKQLLAGYGIE"
     gene            complement(5646..6026)
                     /locus_tag="PLM90_03340"
     CDS             complement(5646..6026)
                     /locus_tag="PLM90_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HMZ88432.1"
                     /translation="MESQAISTQLTDYAAIFGQFIVAAGFVITAIYFAQKLGPKRGGK
                     IKMSSFECGLDSIGNARLPFSVKYFLVAILFVLFDVEVIFFYPWAVNFRELGESGFVK
                     MLVFMAPLLLGYFYIIKKGALKWE"
     gene            complement(6161..6955)
                     /locus_tag="PLM90_03345"
     CDS             complement(6161..6955)
                     /locus_tag="PLM90_03345"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HMZ88433.1"
                     /translation="MDTKDYISSGILELYVTGALTEAERLEVEKMARLYREVKQELES
                     IEMAMEDYAFAHEKTPSPRVLEGILNNIEKTENEQVKILPMSGGSRLYSRFRMLAFAA
                     SILLFLSMVTNIYYYSRYQRVSGELADLRDDNTFLTDQYKSLKANYNGMEKEMAMLHD
                     PAMIALTMKGTAMSPASTSIVYWNKGTGDLYLNANNLPKPADGMQYQLWALVEGKPVD
                     AGVFTMDEGIHQMKLIHEADAFAVTLEPMGGSVSPTLQQLYVMGGV"
     gene            complement(6959..7474)
                     /locus_tag="PLM90_03350"
     CDS             complement(6959..7474)
                     /locus_tag="PLM90_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ88434.1"
                     /translation="MAYIRGLQSGDTEVLKTVYERYAPALLGVISRMVQDDYLAEDIL
                     QEAFVKIWHNASAYDASKGRLFTWMLNICRNLTIDKMRSRGYKAGKATVMDETRLYED
                     PGKIYDAIRPETIGLREMVDKLKPEWKELIDLIYFKGFTQQEVAEALNMPLGTVKTRL
                     RSAIQELRTIF"
     gene            complement(7810..8949)
                     /locus_tag="PLM90_03355"
     CDS             complement(7810..8949)
                     /locus_tag="PLM90_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88435.1"
                     /translation="MIFLSLLLGIIEGKAQIVSDFTSNDESWKVYNEPSGGAPYLPIV
                     WSSTGGNPGGYISETDISIGAWGWLAPSEFKGDLSAYYNFYLSFDLREDSHVGLMDSY
                     YDVMICNASGDTMVFNTTYDPLPINTWVNYIIPLTTAAGWKYGSSIAGGAPAVTAAQM
                     TAILGDVSKMRIRAEFSGLTYETNGMDNVMFTCDLLLPVQLTSFSAEAINNKLSRLFW
                     TTESESNCHGFIIEKSVDFVTFDSIGFIEGNGTTSTPHDYEFYDNNFMTSAYYRLREV
                     NYEGRMFSSDIVYLKNAYSNILTTFVYPNPTDDIITIVADDVNRLFRYTVSDLSGRNM
                     MEVPVGDYNGYFTASISMAQYPSGVYIITMEKKDGVDIQKINLIK"
     gene            complement(9107..9862)
                     /locus_tag="PLM90_03360"
     CDS             complement(9107..9862)
                     /locus_tag="PLM90_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HMZ88436.1"
                     /translation="MNRIKLSEIPTQPPKGMDKSDHEDKTKALIEELGELQNVLFAEG
                     KHSLLVVLQGMDASGKDGVIKEVFSGVNPMGCRVKGFKKPSEEEMKHDFLWRVHKEVP
                     EKGMIQIFNRSHYEDVVIQRVHEWVTLPVIKERYALINAFEKLLQQNGTIILKFYLHV
                     SRDEQQERLKERLSDPRKMWKYNANDLEESKRWDEYMVAYEDAFNTCSPDIPWMVIPA
                     DKNWYKEYLIAKTIVEALKKLDMQYPRIDGQKG"
     gene            9989..12643
                     /locus_tag="PLM90_03365"
     CDS             9989..12643
                     /locus_tag="PLM90_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ88437.1"
                     /translation="MAKKIEEKKRYKFKEMKVYGSLENFFGNQKNYRIVFDETEVCYV
                     NVELQLFNILFDEEEWSAKVKIKASEYYEAKEMCNIDKDITVTPDQNVIIVREGWGTP
                     NTGWWKKGTYKWEIFIDDVLVGTNYFYITNNGAVKPGFNPYFDINAVRLFNSPKGNTP
                     KAERKYFSAFNKDTAQYISVELDLNNKIYTEKYFPLELNLYFYNDLRQLKGACYWFEH
                     INDNRKNVVMDVGYGAENPGFWFEDKYTCEVVFMDTIVAVIPFEVGTTEVPLTGQQVW
                     LTSSDSSTDAFKEAEVMSDEANYDAAKKELDELIGLESVKKEINELTTYLKFLKLRQE
                     KGFEETNRINLNMVFTGNPGTGKTTVARMLGKIYKSLGLLKRADVTEVGRVDLVAEFI
                     GQTAPKTKAAIDRARGGILFIDEAYALSDRGDDNKDFGKEVIEVLIKEMSDGPGDVAF
                     IFAGYPREMRDFLNSNPGLSSRVSQVISFPDYNPDELMAIADYDSKRRSVMIAEDARN
                     LVYMKVVEAYRNRDNKFGNARFVNSIMEEAKQNMALRLMKSENPEALSKDDLSTIILP
                     DIEEIFGLGNSKKVELPIDEVLLQDALGELKALTGMANVKNEIEETVKLVRYYKEIGR
                     DVKRSFSMHTVFTGNPGTGKTTVARILVKIYKALGILERGQLIETDREGMVAGFVGQT
                     AIKADSMIEAAMGGGLFIDEAYALSQGGENDFGREAIEALLKRMEDRRGDFIVIVAGY
                     PDNMAIFLESNPGLKSRFDKFVHFEDYNAEELFTIADNLLQKEYLFLDAAAGEHLKAY
                     ITQLVAARNKYFGNARTMRKLVAEVVHSQNLRMAGIPAAQRTPEMIKTVTLDDVNDFA
                     TDIETKSSIREAIGFKFGQDKGDASQTK"
     gene            12989..15883
                     /locus_tag="PLM90_03370"
     CDS             12989..15883
                     /locus_tag="PLM90_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88438.1"
                     /translation="MQFKFSILGITLFVSAVVSAQWTNTFNGQGDFSDVFQASVADNA
                     GNTYAAGYTINRDVSKDILLAKFNAAGDTVWTRQYAGTGNGPDEALAMLISTDGNIVI
                     TGYQKGAGTGFDFITQKYSTNGNLLWTSFYNYSTNESDQSNAITSDASGNIYIAGQSD
                     KDVSPVNDDDYVVVKYNADGVQQWAKRSDGAIHSTDRPSAIAIGTDGNVIVTGRSSNG
                     SDDDYLTIKYNSSTGNEMWRKLFDRTHHDRATDIAIDPSTGKIFVTGRSNNGTNYDYA
                     TLCYNTSGVEQWQAIFDYVDDDRATNIALDGSGNVYVAGQSDADASTVTINYNFLLVK
                     YNSSGVQQFAQSWDGTAGDDDIPSNLIVDASGNSTITGYSDSDAGVAINNKIAAVRFN
                     AGGVMQWAKTSTIAGSSSLSTSSAMDASGNIIVAGYAESIPNRDACVVKWNNAGVEQW
                     TSTFNGIGDNGDNAHAIIRDNLNNTIVAGYTTSFDQDRNFLIMKISAGGTPVWTRSYN
                     GTSTKQSTDDALSVAVDASNNIYAAGFLKNSGTGYDLLVMKLSTAGDSLWSYIYNDAA
                     SNETDKALSIQMSPSGNLVVCGRSDNDLTAVSDDDILVITLNTSGGVVWTARYQGTGG
                     GDDIPRQMVVAPSGNIYVTGKTTSATDIDALLIKYNTSGVQQWVRKTDIGVEDEFTAI
                     QIDGEENVFVAGNTITATGNFDVITASFDASGNELFTTTYDHGENGNDEAKGLAIFDD
                     GSFAVSCTLAPDTDAITLNGDIAAVYFDTNGNWQSTTIFDGGANDDASESAYASNTGF
                     VICGQTDNGEAGNQDYDYITIYLDETGTITNTELYNGAAGGNDVANTLMLYDNGTYIS
                     GGSEGGQRDIVTILYGSNPVSISNNVASKNVQVYPNPTVATCTVYLDEINLSSSATLK
                     LYGGNGNCVMQKAISSNDNIQLTLSTMPAGMYLLVITDGDQVYSTNLIKQ"
     gene            15910..16959
                     /locus_tag="PLM90_03375"
     CDS             15910..16959
                     /locus_tag="PLM90_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88439.1"
                     /translation="MQARIKKSIICKEITSYQIYSTRNNTYTPIDGDVALFEVLEIGK
                     HQSIQSETKRIVSIFEGDVIMAAFANRYATSQFEGYVPDRPTEILDILGAGGAIGLVA
                     SKNAALEDIEPTKLRLIGFATTTEGQIINTKFLHTARHTFNGYVPNNAKIILSVGSSM
                     DSGKTTSAAFTARGLKTTGKKVAFIKLTGTSFTKDKDFVFDCGADVTVDFTDCGYPST
                     YMCSKEEILDVYQSLLMQLEPHAPDYIVMEIADGLLERETDFLLRDKAFMQTIHSMIF
                     SCGDSLGVFYGLQLLHSFGIVPAVICGRFTMSPLLIQEVKAKTNIPTLDIDQLANGEY
                     IHVFDKSGMQIVHAA"
     gene            16965..18632
                     /locus_tag="PLM90_03380"
     CDS             16965..18632
                     /locus_tag="PLM90_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ88440.1"
                     /translation="MNIIKTYYSRHKGLLISVIGLGLFSNALKLAIPLCVGKFYQLAL
                     DGGSARSGIFDALFGNVQSINIFFSIFFGILLLRFIVQFAEQYLIGMTGETFSAWLRE
                     TLFTKQLHTVYSVFTQKDPGKYLLRYSGDMSALRLFITKGQIGAITDFAFLIMTIGLF
                     GWLNITLTYLLLIAIPAIFLISILINRFMRPSTEKRRDINATNLNFVSERLHAMATIK
                     VMNRSTPEINKFQRRSTKLLEAGKVYQFWNALNKALYPFLLYLLLAFVMLMIYKLLSN
                     NTITADGGTLLIFVMLIIQIIPVVKRLLRSGAIRESGKLSLKKCKLLLEAPEDSREGH
                     AMPVKKGDIRINNLSIKFGGKTIFENASFHFPPKGITLISGTGGSGKSTLLKYISGLT
                     IPESGEIMIDDASTKSYSIFQVRKKITFISQELPLIGKHVYDAISYNRDPDKKHDAMQ
                     ILSAIGFCDKFQDEVLELPLEKGGANFSTSQQQLLRIARGLITGKKIILLDEPFEGLD
                     DRSARQVAYHLTHISSSHTIILVSKSIPETLFIQHRISLDKLKQPNV"
     gene            18625..19029
                     /locus_tag="PLM90_03385"
     CDS             18625..19029
                     /locus_tag="PLM90_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88441.1"
                     /translation="MYKLTILVIGIILISSCTKDPGIGGEATITGKVHALHYNSTFTV
                     LLSEYDLADTYVYLVFGDDINYGTRIKTNYDGEYAFEYLYPGDYTIYTYSIDSAATVN
                     GIPTPPDSAVIRQITISDKNALVETEDLVVFK"
     gene            19036..19584
                     /locus_tag="PLM90_03390"
     CDS             19036..19584
                     /locus_tag="PLM90_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88442.1"
                     /translation="MKIIIAGCIILSSLQLFGQNTRGAKTMYQATREIEAGKQVTVKH
                     VFQSFDNKGRTTEEWTLKGDSTFASRDQWTYNRKGLETKHIIFKDSLNIESIISTSYD
                     RFEVKKLSITTDGKGSIIEQTAYHYNTFGKKIEELTSDSGGNVTKKITFSYDSHGALA
                     ERIIYDGKNNIVSSKQYNYTYR"
     gene            19619..20254
                     /locus_tag="PLM90_03395"
     CDS             19619..20254
                     /locus_tag="PLM90_03395"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HMZ88443.1"
                     /translation="MPLCLNVHAQQSDAVFTSGISVEKKITRTWSASTFIQSGFNENY
                     SELGYLLIDGGLGLRLNREWSLEGHYRYGSVRNVANSYDTRQYIYGDVQYVKGIGDFT
                     LIARSRYMVKTYGLYISDENQYRDNKHFLRNRIQLKYDLGPYSDIFLATEQVYRLDDH
                     NESEQFRFSGGYNYTFNVHHRIQLQYTVAQQVNKKAPDTDYITGVTYYFKF"
     gene            20264..21334
                     /locus_tag="PLM90_03400"
     CDS             20264..21334
                     /locus_tag="PLM90_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HMZ88444.1"
                     /translation="MKHYITAICLLAGSLLFTSCDKSSNGGKSDTSTPKFSIAIHGGA
                     GALAKGSYTAEQEAAFKAKLEEATKLGYEMLSKGDSAADVVVAVIKILEDSPLFNAGK
                     GSVITYNGKIKMDAAIMNGRTLDCGTVNNVQRIKNPITAARMVMDSSKYIMFSSAGAE
                     SFAAKNGIEMIDKSYFYTQHQIDRWRKTKDSTDASYIHYVDSMAALQSPIISALPEEG
                     EFGTVGCVVMDKYGNLAAGTSTGGLINKKYNRVGDSPIIGAGTYANNKTCAISCTGTG
                     EDFIKTVAAKTVSDLIEFKGMTLDAATYDMIHVRFKEITGDGGMIAVDKYGNISWQYN
                     SDGMFRARTDQNGKIETYIYAE"
     gene            21374..22306
                     /locus_tag="PLM90_03405"
     CDS             21374..22306
                     /locus_tag="PLM90_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88445.1"
                     /translation="MRIRRYVPANQLIYSSLGLAFILCIFASCSDSKDPVIAYTKSMY
                     VLNKDEEELRSVTDYDSIDRIIAEYIIGRKGRDTVEKSTYVYGDDGLIRNTTFSNDKI
                     THQQFYVYAGSMLMQMIETDGHDTIGITDYVYYPTGKIKRTVLQLVYDKSSPTIIVTN
                     YNRHGDKETMYKQIYEDSSRLVLSRYEMDKFISTYDSSEKMIRNIDQFFLGYYEEADT
                     TLDVSYQYDDAGRLIVERNAKRIDTSVPDSIFYFYNAAGLLQEKLVFTSSAHHREKVV
                     TTDRVQYDYDKNGRLIKEYSFYYKTGRRYSYRAK"
     gene            complement(22355..25498)
                     /locus_tag="PLM90_03410"
     CDS             complement(22355..25498)
                     /locus_tag="PLM90_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HMZ88446.1"
                     /translation="MTKKRPTGRNTLLLALTFASAALSGQSALWTNRFNGTGDNSDRY
                     NQAIADGLGNFYCAGYTVNPGKGKDFYTVKLNSAGDTVWTRTYNYTSNLDDEANYIAL
                     TPAGEIVVCGYSDGGSASTKTDVLTLKYSATGTLLWTARFNEVTTNEDELPAGLAIDN
                     AGNIFIGGRSDHDALNVDDFITLKYTSAGVLSWSARFDNGFTDRAAGIVADQSGGCIV
                     TGRSNNGANDDMLTISYNSLGTVSWSALFAGPGGDDRGQSIARDAAGNVYVAGLRANA
                     SDDDYLTVKYNSSGIEQWTKVFNGGDNDRVSLLKLDGAGNVYVTGQSDIDPSGGNSDY
                     NFRTIKYDPTGVVVWNTLSGNPVLQEDTPTDMFIDASGNIYVTGKSDAIGGANVNFEF
                     MTIKYNSAGAQQWAAYFDGTTSNAEDIPATVLTDASSNVWVFGAANFTATQKDCTALK
                     YTAAGVLSATKTINGKGDFNDKVNALTTDASGNSIVVGYTMAAGQQRNILIQKISPAG
                     TTNWTRTFDGTNENDEALAVTTDAAGNTYLAGYSNGSGTYDDYIVIKYSTLGVALWTV
                     IFDHTAHQLDKAVSIVVSDAGDVYVTGYSDGDASALVTNYDITTIKINALGLLQWTAR
                     YNGVGNAADKPAEILFDGSNVLVAGTTFNGANNDIVLIKYDILGTQLAAVTYNGASNG
                     DDAAEDMLIDGSDVYVTGSSFVNTNMDDYVTIKYNSTFVKQWEKNYNGPGDGVDVANG
                     ITVAGAEVYVTGSSVGTSGAADLALIKYNKTTGAQNWAKRYTGAGAFADEGVSVTSDG
                     VDNIYTTGPSGNATSVSDFITLDYNPAGAKLLTLKYNGPGNGEDVAKEVKLDNNGYLF
                     VAGHGTGTGKNNFDFTTIKYCTPVPAASITALGATTICSGSSVTLSANTGTGLTYQWK
                     KGNANIAGATASTYAATTAGGYKVVITNANGCSKTSNVINVSVIICREEQIEIAPVSV
                     NVSPNPFTDYAVIDCSNITMQTEIVVLDITGRTVSTVIAEPGTGSVEIGQDLPQGNYL
                     LVVNHGSQMQTFRIVKM"
     gene            complement(25815..26141)
                     /locus_tag="PLM90_03415"
     CDS             complement(25815..26141)
                     /locus_tag="PLM90_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMZ88447.1"
                     /translation="MIFISEPAKHKLESLKSEAGIGSDYFIRVSVVGGGCSGLSYKMD
                     FDNEEKPGDQIFEDRGEKLVTDLKSFLYLCDTTLDFSDGLNGKGFHFVNPNASRTCGC
                     GESFAV"
     gene            complement(26197..26616)
                     /gene="iscU"
                     /locus_tag="PLM90_03420"
     CDS             complement(26197..26616)
                     /gene="iscU"
                     /locus_tag="PLM90_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HMZ88448.1"
                     /translation="MAYSKKVLEHYENPQNVGTLDANKQEVGTGLVGAPECGDVMRLQ
                     IEVDPETKVIRDARFKTFGCGSAIASSSVATEWLKGKTIDEALTIDNMDIVEELALPP
                     VKIHCSVLAEDAIKAAINDYRKKNGMPELQLDEKIVH"
     gene            complement(26656..27873)
                     /locus_tag="PLM90_03425"
     CDS             complement(26656..27873)
                     /locus_tag="PLM90_03425"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HMZ88449.1"
                     /translation="MLKLPIYLDNSATTPVDPRVVDTMVPYFYEHFGNAASRSHPFGW
                     QAEDAVDQARERVAKLVNASDKEIILTSGATESDNLAIKGVFDMYGKKGNHIVTVTTE
                     HKAVLDTCKHLEKLGAQITYLKTDNEGNIDLNELESVVTDKTILVSVMYANNEIGTIA
                     PIRQIADIAHKKGSLLFTDATQAAGKISIDVERDGIDLMAFSAHKMYGPKGVGALYVR
                     RKNPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKAAELAMLEMDADAARLSVMRDR
                     LENALMQMEEAYVNGNRKSRLPHITNISFKYVEGEGLMMAFNQNIAVSSGSACTSASL
                     EPSYVLKALGLGDDLAHSSIRFSLGRFNTDEQVDYTIEAVTKAVNKLREMSPLWEMFK
                     QGIDLSTIEWAEH"
     gene            28161..28562
                     /gene="mce"
                     /locus_tag="PLM90_03430"
     CDS             28161..28562
                     /gene="mce"
                     /locus_tag="PLM90_03430"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HMZ88450.1"
                     /translation="MKKIEHIGIAIKALESSNALFAKLFGKDHYKVESVEREGVSTSF
                     FMMGETKIELLEATNPESTIAKFIEKRGEGIHHIAFAVDDIYAEMHRLESEGFQLLHK
                     EPLPGADNKLIAFLHPKSTNGVLVEICMERK"
     gene            complement(28571..>29188)
                     /locus_tag="PLM90_03435"
     CDS             complement(28571..>29188)
                     /locus_tag="PLM90_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ88451.1"
                     /translation="TNYKDRNRIGTVGLRFPGVELKFSEQGEMLVKHPAMMLGYYKED
                     ALTQEVFDEEGFFKTGDKAEVDADGFLKITGRVKELFKTSKGKYVAPMPIEMKFANNT
                     DVGQVLVVGYALPQPIALVTLSENGKKKSKQEVSDDLVKTLKAINATLDSFEKLEKVV
                     VLNEEWTIANNLLTPSLKVKRNEVEKKFQDNYEKWFEMKDIAIWQ"
BASE COUNT         8667 a         6223 c         5794 g         8504 t
ORIGIN      
        1 atcaccgaat gacgatctac cggtgcagga ccttcgattg tccagtcgga agtattcttt
       61 ttatcgaatc tgcattcgtt acaaatgaag ccttcacttt ctccccattt atcttttctg
      121 ctggttacgc gaagcacttc ttctccctga taccacaaag caactttacc gctgcatgtc
      181 gggcaattgc gatgtgcata cattggttta gtgaaccata cacgcgattt aaaccggaat
      241 gtcttatcgg taagcgcacc aacaggacaa acatctatca tatttcccga gaattcattg
      301 tctactgcag ttgcaataaa tgtagagatt tcggatactt caccgcgata taatacaccg
      361 tgtttacgtt ccggggtaag ttgttctgca acttgtgtac agcgataaca caagatgcat
      421 ctgttcatat gcaattgtat tttatcgccg atgtcgatgc gttcgaatgt acggcgttca
      481 aaatcatatc tgctttttga caagccgtga tcgtagctta agtcttgcaa atgacattca
      541 cccgcctgat cacaaatagg acaatcgagt ggatgattta caagcaagaa ttcaacaact
      601 ccgcgacgag cttcaatcac ttcaggagaa gtaacattgc gcacttccat accatccatt
      661 acggttgtgc ggcagcttgc taccagcttt ggcataggac gaggatcttt atcggatcct
      721 ttcgtaacct tcaccaaaca aactcggcat ttaccaccgc taccttttaa tttggaataa
      781 tagcacattg ccggtggcac attcacaccg atctgacgtg ctgcttccaa tattgttgtt
      841 ccggcaggaa cttcaatggt gatatcgtct attgtaacct ttggcatttt tattatatgg
      901 ttatctgcaa attatctcaa aactattttc ttaaattaat ttcattcaat attatactgt
      961 ttgcattttc atttccggtt gatacacatg atcagcatca aaaacacgat gcggatattt
     1021 cacgtgatat tcgaactcgt gacggaaatg tctgatggca gcagctacag gccatgcagc
     1081 ggcatcaccg agcggacaaa tggtatttcc ttcaatcttg cgttggatat cccacagcaa
     1141 gtcaatgtct tccatttttc cgtgaccgta ttcaatatta tgtaatacgc gttccatcca
     1201 tcctgttcct tcgcggcatg gtgaacattg tccgcaagat tcatgatgat aaaagcgcgc
     1261 gaaattccag gtattgcgaa cgatacatgc tttctcatcg aaaacaataa atccgcctga
     1321 acctaacatt gagcctgttg caaatccacc ttcgcttaaa ctttcataag tcattaatcg
     1381 aggggtgcca tcgctctgtt taaataacaa atcttttgga agaataggca ctgaagaacc
     1441 tccgggaacg catgctttaa tgtcgagacc gttggcaatt cctccgcaat attcatcgct
     1501 ataaataaat tcttctactg atacgccaag ttcaatctca taaacaccgg gtttattaat
     1561 attgcctgat gcagaaatca attttgttcc tgtagatttt ccaattccaa ttttagcata
     1621 atcttcacca ccgttattca cgatgggcac aactgctgca attgactcaa cattatttac
     1681 aatggttgga caattatata atccggcgat ggcagggaat ggtggtttaa tgcgcggatt
     1741 accacgcttt ccttccaatg attcaatcaa cgcagtttct tcaccacata tataggcgcc
     1801 tgctcccgga tgcacatgca aatcgagaga ataaccggtt cctaaaatat tttgtccgag
     1861 gtatccttta gcatacgctt ccgcaattgc cttttcaaga atgcggagga tgaacatata
     1921 ttctccgcgg atataaatat atgaagtgcg acaacccaaa gcgtagcttg aaacaatcat
     1981 accttccacc aacaggtgtg gtattttttc catgagcaag cgatctttaa aagtacccgg
     2041 ctcagattca tcggcattgc atagcaggta acgcggtttg tcggtagact tatcaagaaa
     2101 cgaccacttc attcccgtag gaaaacctgc gccgccgcgt ccgcgcaatc cggatttctt
     2161 cacctcttcg gtaacttcat ccggcgtcat agacttcaac gctttttcca atgcgcgata
     2221 tccgccatat ttgatataac catcaatcgt gttgatctcc ggcttatcca aattttccaa
     2281 taataatttt cgtcccacta tatttttaat tagcgaatga gctaatacta tgtttaactg
     2341 tttttgttgc gcatttccaa aagaatatta tctacttttt cttctgtcaa atgctcataa
     2401 aatgtttctc caatttgaaa cattggtgca tatccgcaag cgcctaaaca ttccaccgtt
     2461 tttaaagtga acatgccgtc gcgtgtagtt tcaccaactt ttatctgtaa ttttttttct
     2521 aaataggata caattttctc tgcaccattg agacaacaag gtcctgtttg gcaaacttca
     2581 atcagcacct tgccaacggg ctttgtattg aacatggaat aaaacgttgc cacctcatac
     2641 acttcgatag gatgtatatg taacagacga gccacataat ccatagactc cgaactcagc
     2701 cagccaccaa attccttctg cgcaatgtgc aatacaggca ataatgcaga cttctgcttg
     2761 tcggcgggat agtgactaat aatcctaccc acttccatca tggcttcagg tgataattca
     2821 atatgttttg cttcagtcat tataactgtt taatattttt cttctttcta aaatcaattc
     2881 tctcaatact catgtgttcg tgtgcttgtg tgtttgtttc cctccactct ttgctgctta
     2941 acgaaaccta tcccacctcc taacttgcca acctgctaac tctcaaactt gcaaaccaaa
     3001 ctaaaatctc cctacgcttc taaacccttt ttgcaaattc cgatataaaa cacaacgaca
     3061 gcccacccct accaaatctc cccctctctc ctctctcctt tttcctttct cctctctcct
     3121 ctaatcaagc atccaactcc cctgcaatca cattcatcga actcatagtg ataatagcat
     3181 cactcaacat cgctccttta atcatttcag gataagcttg ataatatata taacatggtc
     3241 tacggaaatg gagtcgatat ggctgacgac caccatcgct taccaaataa aatccaagtt
     3301 caccgtttcc gccttccaca ctatgataca cttgtccttt cggcacatgc acttcaccca
     3361 tcacaatttt aaaatgccag ataaggcctt ccatggtatt gtaaacatcc tgtttcggag
     3421 gaagatagaa ttcaggaaca tcacctttaa aatctccgga aggcattttt tgcatcgctt
     3481 gtttgatgat gtgcaaactc tgccacattt cttcttcacg cacacagaat cgatcataag
     3541 tatcgccatt agtgccgaca ggaataataa aatcgaaatc ctggtaacta ctgtatggcg
     3601 acatcacacg cacatcataa tctacaccgg ctgcgcggag gtttggacct gtaaatccgt
     3661 atgccaatgc tcgttcaccg ctgatgggtc ctacttttat agtacgatcc atgaaaatgc
     3721 ggttgcgcat taccattttt tcgaattctt taagtgctgc agggaattca tcaacaaact
     3781 cctgtattct tcgccatacg tcatcagaaa aatcgcgttc gaagccaccg atgcgaccga
     3841 tatttgtggt aaggcgtgta ccgcaaatca attcataaat ttcatagatt ttttcacgcc
     3901 attgaaatac atataaaaat ccggtgagtg ctcctgtatc tactgcgata actgaattac
     3961 aaataatatg atcggcaatg cgtgctaatt ccatgataat cacacgcatg taatccacgc
     4021 gttttggaat ttctgctttt ataagttttt caaccgtcat atgccatccg atattattga
     4081 taggcgaaga gcaataattc aatcggtcgg taattggtgt tacctgataa tatggacgat
     4141 gttcggcaag tttttcaaat gcgcgatgga tgtaaccgat ggttggctgt gcatcaacaa
     4201 tgatctcgcc atccattttc agaatattct gaaaaatacc atgcgtagca ggatgtgtag
     4261 gaccgaggtt aaggtaatta tactgtttta attcctcctc tgtctgcacg acaccgttaa
     4321 taatattttc gttttttgcc atgttatggt agtgagtagt ggatattgag tattgagact
     4381 taatcccgag ctcacttatt ttattttatt aacttgtaac ctaactcacc cttccccatt
     4441 ttcacccctc ctcacattcc cacatggagc ttagcgacat ccctcctcaa cgggattccc
     4501 acactcccac attcccacat tctcacattc ccacactttc acacctgcac acttccacac
     4561 ctgcacactt ccattctcac cttccaaagt acttatcttc tttatcagtt ctcgttccat
     4621 cttcaagtgg atattgtttc aacaagggat gatattgcat gtcttccaca ttcaaaattc
     4681 tacgcaagtc gggatgtcct gtaaagcgta taccaaagaa atcgaatgct tcacgttcca
     4741 tccagtttgc tgttggaaat aattcgatta ccgaaggcgc attagcatcg tttgcgggga
     4801 aaaaaatgtg taaacgaatg cgcacattat ttataaagct gtgcaggtga taaacaaccg
     4861 ataattcagc atttttcact tcaggaaaat gcataccgca aagactggta aggaaattaa
     4921 attgcagaat actatgatcg cgcaaccagt gtagtatttc aacaatttta tctcgtgaca
     4981 ccgtaatgtc gagcatgccg tatgattctt cagatgcttt tatggcatcg ccaaactgtt
     5041 cgcgcagcag gctttctacg aggtgatttt ctattttatt ttccattact cgattccgta
     5101 gccggctaat aattgtttat attcaggtga atctcgacgg cggaagcctt cgattttcac
     5161 gagttcttga atttttatta atccatcgag gatagcttcc ggaggaggag gacaacccgg
     5221 cacatacaca tctacaggaa ttactttatc tattccttgc aaaacagaat aagtgtcgaa
     5281 aattccaccg ctggaagcac aagcaccaac agcgatcacc caacgtggtt cagccatttg
     5341 tgtgtatact tgtttcagca cggggcccat tttttttgaa atagtgccca tcaccatcag
     5401 gagatcagct tgacgaggtg agaaactcaa acgttcggca ccaaaacgtc cgaggtcgta
     5461 tgttgccgcc attgttgcca tgaattcaat tccgcagcag ctggtagcaa acggcagagg
     5521 ccaaattgaa ttggcacgtg caagtccaac tactttttca agcttggtga gcgcataacc
     5581 ctcacctgta tacatttcgg ggatctcatt taccttttgc agcgccatga attacaaggt
     5641 tggttttatt cccatttaag ggcacctttt ttaatgatat aaaaatagcc cagaagcagc
     5701 ggtgccatga acaccaacat cttcacaaat cctgattctc ctaactcacg gaagttaaca
     5761 gcccatgggt agaagaatat tacttcaaca tcgaataaca cgaataggat cgctacgagg
     5821 aaatatttaa cagaaaatgg caaacgcgca tttccaatag aatcgagtcc gcattcgaaa
     5881 ctcgacattt tgattttccc acctcttttg ggacccagtt tttgggcaaa ataaatagca
     5941 gtgatcacaa atcctgcagc aacaatgaac tgtccgaaaa tagctgcgta gtctgtgagt
     6001 tgagtagaaa tagcttgtga ctccatttgc ttattgtcat tcagccgaaa acacttgttt
     6061 taggcacggc aaaggtagga aaacggtacc ggaaatacca tgattaaaaa agcacaaatt
     6121 tcgggtcttt gtgacattgt ctgttcagaa aacagaacgt tcagacacca cccataacat
     6181 agagttgctg tagtgtggga gatacgctgc ctcccatagg ctcaagggta actgcaaagg
     6241 cgtctgcttc gtgtatcagc ttcatttgat gtatgccttc atccatggta aatacccctg
     6301 catcaacagg tttaccttcc acaagtgccc agagttgata ttgcatacca tctgcaggct
     6361 ttggcaaatt gttagcattg agatacaggt cgcccgtgcc cttattccag tatacgatgg
     6421 aggtggacgc cggactcatg gctgttccct tcatggtaag ggctatcata gccggatcgt
     6481 gcagcatggc catttctttt tccatgccgt tataattggc tttcagcgat ttatattgat
     6541 cggtaaggaa ggtattatca tcccgcagat cagccagttc accagaaacg cgctgatagc
     6601 gactatagta gtagatgttt gtgaccatag acaggaacaa caatatactg gcggcaaagg
     6661 ccagcatgcg gaaacggcta tataagcggc ttcccccgct cattggaagg attttaacct
     6721 gctcgttctc tgttttttct atgttgttga ggatgccttc caacacacgt ggcgaaggag
     6781 ttttttcatg tgcaaaggca tagtcctcca ttgccatttc aatggattca agctcttgtt
     6841 tgacctcgcg atacaagcgg gccattttct caacctcaag cctttcagct tcggtgagcg
     6901 cgcctgttac gtagagctcc agtattccgg atgatatgta atccttggta tccacttgtt
     6961 aaaatatcgt tcttaattcc tgtatagcac ttctcaatct ggtttttacc gtccccaagg
     7021 gcatattcag cgcttccgcc acctcttgtt gggtaaatcc tttaaaataa atcaggtcga
     7081 tgagttcctt ccactccggt ttcaacttat ctaccatttc tcgcaaccca attgtttctg
     7141 gcctgatggc atcatagatc ttgcccggat cttcatataa acgggtttcg tccataaccg
     7201 ttgcctttcc tgctttgtag cccctgcttc gcattttgtc gattgtgagg tttctacaaa
     7261 tattcaacat ccacgtaaac aacctccctt tagaagcatc ataagctgat gcattgtgcc
     7321 agatctttac aaaggcctcc tgcagaatat cctctgccag gtaatcatct tgcaccatgc
     7381 gacttataac ccccaaaagt gcgggagcgt agcgctcata aacagttttc aatacctcgg
     7441 tgtcgcccga ttgtaaacca cgaatgtacg ccacttcgtt tgcttgtatg ttcgaagacg
     7501 gtgtcaatca gatgtctgta acttggcaaa tctacgaggg aaatgttcca ttggatttat
     7561 ttgctttaaa ttttcttgag gaatttttga aatttccgag gttagcccct gttaagggat
     7621 gaaatatttt attataacgc attgaaattc aatagattac gaaaaaattg cttcacttct
     7681 gatttgtctt agtgcaaaca atttattatt tagtcaatgt gaaacgaaga cggttgactg
     7741 ccgccctaaa attggataca aactagccgg aaggcgaatt tacagtaaat gaaaggagcc
     7801 cttaatcagt tattttatca gatttatctt ctgaatatca acaccatcct tcttttccat
     7861 ggttatgata tacacgccgg aaggatattg ggccatactg atgcttgcgg taaaatatcc
     7921 attgtagtca ccaaccggaa cctccatcat atttcttccg gacagatctg aaacggtata
     7981 cctgaataat cggtttacat catcggctac aatggttatg atatcatccg ttggattagg
     8041 gtaaacgaat gtagttagaa tattactgta tgcattttta agatacacaa tatcagaaga
     8101 gaacattctt ccttcataat tcacttctct caatcggtaa taagccgagg tcatgaagtt
     8161 gttatcgtaa aactcgtagt cgtgaggcgt agacgtagtg ccattacctt caataaatcc
     8221 tattgaatcg aaggttacaa aatcaacgct tttttcaatt ataaatccat ggcagttcga
     8281 ctcactttca gttgtccaga aaagcctgga aagcttgtta ttaatggctt cggcagaaaa
     8341 ggaagtcagt tgaacaggca atagtaggtc gcatgtaaac atgacattat ccatgccgtt
     8401 ggtctcatag gtaagacccg agaattcagc tctgatgcgc attttactga catcacccag
     8461 aatagcagtc atttgtgcgg ctgtcacagc aggagcacca ccggcaatag acgaaccgta
     8521 tttccatccg gcagcggtag ttaacggaat aatgtaattg acccatgtat tgatgggaag
     8581 tggatcgtag gtagtattga agaccattgt atctccggaa gcattacaga tcatcacatc
     8641 ataatagcta tccatcaacc caacatgcga gtcttcgcgt aggtcaaatg acaaataaaa
     8701 attgtaatag gcagagagat cgcctttaaa ttcagagggt gcgagccagc cccaggcgcc
     8761 aatagaaatg tcagtttcac tgatatatcc gccgggattt cctccggtag atgaccagac
     8821 aatagggaga taaggggctc ctcctgaagg ttcgttgtat accttccaac tttcgtcatt
     8881 ggaggtaaaa tcgctgacta tctgcgcttt tccctcaata atccccaata aaagggacag
     8941 gaaaatcatt gaaataccta tatgtttttt cgggtatgcc aacctgaaga gcagttttag
     9001 cgttaaaata gcatacaaga atacatcgct cctacattaa atccaaaaat ttttcactaa
     9061 attttcattc atgcaacggc ctcaaaccta acggcatcta aggagtctac cctttttgac
     9121 catcgattcg aggatactgc atgtcgagtt ttttcaaggc ctccactatc gtcttggcta
     9181 tcagatactc tttataccaa tttttgtcag ccggaatcac catccagggt atgtccggag
     9241 agcaggtgtt gaaggcgtct tcataggcta ccatgtactc atcccatcgc ttactttctt
     9301 ccaggtcatt ggcattgtat ttccacatct tccttgggtc tgaaagcctt tccttcaggc
     9361 gttcctgctg ttcgtcgcga ctaacatgca ggtaaaattt cagaatgatg gtgccatttt
     9421 gttgcagcaa cttttcgaag gcattaatca aggcataccg ctcctttatg acgggcaaag
     9481 taacccattc atgtacgcgc tgaattacaa catcctcgta atgagagcgg ttaaatattt
     9541 gaatcatccc cttttcaggc acttctttat gcactcgcca caaaaagtcg tgtttcattt
     9601 cctcctcact gggtttctta aagcccttaa cacggcatcc catagggtta acaccactga
     9661 acacttcctt gatcaccccg tctttgccgc tggcgtccat accctgaagt acaactaaga
     9721 ggctgtgctt gccttcggcg aacaatacat tttgcaattc acctaattcc tctatcaggg
     9781 cttttgtttt atcctcatgg tcgcttttgt ccatgccctt gggtggttgt gtcgggattt
     9841 ctgacagctt aatcctattc atcccgtaaa tgtatggatt tgaccttatg attgaaaaat
     9901 gagcttaact aactacaaca aaatttgttg accaaacttt tagggaatgt ttatttttac
     9961 aacccattac cccaaattta aaagcattat ggctaagaaa atagaagaaa aaaagcggta
    10021 taaattcaaa gagatgaaag tgtatggttc gctggaaaat tttttcggta accagaaaaa
    10081 ttatcgcatt gtatttgatg aaacagaagt ttgctatgta aatgttgaat tgcagttatt
    10141 caatatttta ttcgatgaag aggaatggtc ggcaaaggtt aaaatcaaag caagtgaata
    10201 ttatgaggcg aaggagatgt gcaatatcga taaggatatt acagttaccc ctgatcagaa
    10261 tgtaattatt gttcgcgaag gatggggtac accgaatacg ggttggtgga aaaagggaac
    10321 ctacaaatgg gagatattta ttgacgatgt acttgttggc actaattatt tttatatcac
    10381 taacaatggt gctgttaaac ccggtttcaa tccttatttc gatattaatg ccgttagatt
    10441 atttaattcg ccaaaaggaa acacaccgaa agctgaacga aaatattttt ctgccttcaa
    10501 taaagatacg gcacaatata tttccgttga gcttgatttg aacaataaaa tctatacgga
    10561 aaaatatttc cccctggaat taaatttata tttctacaat gacctccgtc aattaaaagg
    10621 tgcctgttat tggttcgaac acataaatga taatcgcaaa aatgtagtca tggatgttgg
    10681 ctacggcgct gaaaatcccg gtttttggtt tgaagataaa tacacctgcg aagtggtatt
    10741 catggatacc atagttgccg taatcccatt tgaagttggc acaacagaag tgcctctgac
    10801 cgggcagcag gtgtggctta caagcagcga cagtagcacc gacgcattta aagaggctga
    10861 ggtaatgtcg gacgaagcaa attacgatgc tgcaaaaaaa gagctggatg aattgattgg
    10921 tcttgagtcg gtaaaaaaag aaatcaatga gcttactact tatttgaaat ttttaaagct
    10981 gcgtcaggaa aaaggatttg aagaaacaaa tcgcataaat ctcaatatgg tatttaccgg
    11041 taatcccgga acaggaaaaa caactgttgc acgcatgttg ggaaaaattt ataaatcatt
    11101 aggtttattg aaacgcgctg atgtgacaga agtaggacgc gtagatttgg ttgctgaatt
    11161 catcggccaa acagctccaa aaacaaaagc ggctatcgat cgcgcacgtg gtggaatttt
    11221 atttattgat gaagcttatg ctttgagtga tcgcggagat gacaataaag atttcggaaa
    11281 agaagttatc gaagtactga ttaaagaaat gagtgatggc cccggcgatg tggcatttat
    11341 ttttgccgga tatccacgtg aaatgcgtga ttttttgaat agcaatccgg gattaagttc
    11401 acgcgtttca caggtaatca gttttcctga ttataatcct gatgagctga tggcaattgc
    11461 cgattatgat tcaaaacgtc gatcagtgat gattgcagaa gatgcgcgca atcttgttta
    11521 catgaaggtt gttgaagcgt atcgcaatcg cgataacaaa ttcggaaatg ctcgctttgt
    11581 caattctatc atggaggagg ctaaacaaaa tatggccttg cgattgatga aatcagaaaa
    11641 ccctgaggca ttaagcaaag atgatttgag caccatcatt ttaccggata ttgaagaaat
    11701 cttcggactc ggcaattcta aaaaagttga gctgccgatc gacgaggtat tgttacagga
    11761 tgcgcttgga gaattaaaag cgctgaccgg tatggcaaat gtgaaaaatg agatagaaga
    11821 aacagttaaa ctggttcgat actataaaga gattgggcgc gatgtgaagc gcagtttctc
    11881 aatgcacaca gtctttacag gaaatcccgg aacaggtaaa acaactgttg cccgtattct
    11941 tgtgaagatc tataaggcgc tgggaattct tgagcgtggt caattaattg aaaccgatcg
    12001 cgaaggaatg gttgccggtt tcgtgggaca aacagccatc aaagcagata gcatgatcga
    12061 agcagcgatg ggcggtggtt tatttattga tgaggcctat gctttgagtc agggcggtga
    12121 gaatgacttc ggtcgcgagg caatagaagc tttattaaag cgaatggaag accgtcgcgg
    12181 agacttcatc gtcattgttg ccggatatcc cgataatatg gctattttcc tggaatccaa
    12241 tcccggatta aaatcgcgct tcgacaagtt tgtgcatttt gaagattata acgccgaaga
    12301 gctattcacc atagccgata acctgcttca aaaggagtat ttgttcctag atgcagctgc
    12361 cggagagcat ctgaaagctt atatcaccca attagttgct gcccgaaaca aatattttgg
    12421 caatgcccga accatgcgca aactggtagc cgaggttgtg cattcccaaa atttgcgtat
    12481 ggcaggtatt cctgctgctc agcgcacacc tgagatgatc aaaacggtta cccttgatga
    12541 tgtgaatgat ttcgcgacgg atattgaaac aaaatcatca attcgcgagg ctatcggttt
    12601 caaattcggt caggacaagg gcgacgcctc ccaaaccaag tgaccttagg tcatcagagt
    12661 ggagtgagat acgtatttat acatatatta tatatgtata gacatgtttt taactgtata
    12721 tacaggtatc ttctgccgtt ttttcatgga aaaacaccct ttttcagccc ttgatttgtc
    12781 gaaaagggca tttttgtatc ggagaaaata gggcgaggta atacggcaag atgccctatt
    12841 tgcaggcatt tcaggcgaaa gcccctcacg tcaacacgac aaatacaggc ggaaaaacgc
    12901 aaaaatcgag ggaaaatgag gtctttttgt ccttgagtgg ggtttagaac cgaaatactt
    12961 ttggtttcgg aactgaacaa tccaccacat gcaattcaaa ttttcaatct taggcattac
    13021 cttatttgta tcagctgttg tgtcagcaca atggacaaac actttcaacg ggcaaggcga
    13081 cttcagcgat gtatttcagg cttcagttgc tgacaatgcc ggcaatacat atgcagccgg
    13141 gtataccatt aatcgcgatg tttcaaaaga tattctactg gcaaaattca atgcggcagg
    13201 agatactgta tggacacgtc aatatgccgg caccggaaat ggaccggacg aagcgttggc
    13261 aatgcttatt tcaacagatg gcaatattgt aatcaccgga tatcagaaag gcgccggaac
    13321 aggatttgat tttattaccc aaaagtatag caccaacgga aacttacttt ggacatcatt
    13381 ctacaattat tctaccaacg aatctgatca aagtaatgct attacttcag atgcatcagg
    13441 caatatttat atcgccggtc agagcgataa agatgtgagt ccggtaaatg atgacgatta
    13501 tgtagtagtt aaatataatg ccgacggtgt gcagcaatgg gcgaagcgtt ccgatggtgc
    13561 aattcattca accgacagac catcagcaat tgcaattggc acagatggaa acgtaattgt
    13621 tacgggcaga agtagcaatg gtagtgatga cgattatctt actataaaat ataatagcag
    13681 cacaggcaat gaaatgtggc gaaaattatt tgatcgcacg caccacgatc gtgctactga
    13741 tattgcaatt gatccaagta ccgggaaaat atttgttacc ggtcgcagta ataatggcac
    13801 gaattacgac tatgccactt tatgttacaa cacatcagga gtagagcaat ggcaagccat
    13861 atttgattat gtagatgatg atcgcgcaac aaatattgcc ctagacggat ccggaaatgt
    13921 atatgtagcc ggtcaaagtg atgctgacgc ctcaacggtt acaataaatt ataatttcct
    13981 gcttgtaaaa tacaatagct caggtgttca acaatttgcg caatcgtggg atggcacggc
    14041 aggagatgat gatattccat caaatttaat tgttgatgct tcaggaaatt ctaccataac
    14101 cggatatagc gatagtgatg ccggggttgc aatcaacaat aaaattgcag cggtaagatt
    14161 taacgccggt ggagtaatgc aatgggcaaa aacttcaacc attgcaggaa gcagcagttt
    14221 gtcaacatct tcagccatgg atgcatcggg caatattata gttgcaggat atgctgaatc
    14281 cattccaaac cgcgatgcat gtgttgtaaa atggaataat gccggcgttg aacaatggac
    14341 atcaactttc aatggcattg gcgataatgg agataatgcg catgcgatta ttcgcgacaa
    14401 tttaaacaat accattgttg ccggatatac aacaagtttt gatcaggaca gaaatttcct
    14461 gatcatgaaa atcagcgcag gaggcactcc tgtctggaca cgcagttaca atggcacaag
    14521 tactaagcaa agcactgatg atgcattgtc ggtggcagta gatgcaagta ataacattta
    14581 tgctgcaggg tttctaaaaa acagtggcac aggatacgat cttctggtta tgaaattatc
    14641 tacagcaggt gatagcttgt ggtcatacat ttataacgat gcagcctcga acgaaactga
    14701 taaagcgctg agtattcaaa tgagcccaag cggaaatcta gttgtttgcg gtagatcaga
    14761 taatgatctc acagcggtat cggatgatga tattctggtg atcacattaa atacatcagg
    14821 aggtgtggta tggacagcac gttatcaggg aaccggtgga ggcgacgaca ttccgcgaca
    14881 aatggtcgtt gcgccatccg gcaatatata tgttacaggt aaaacaacat cggctaccga
    14941 tatagatgct ttgctgatta aatataacac cagcggagtt caacagtggg taagaaaaac
    15001 ggatatcggg gttgaggatg aatttacggc tatacaaatt gatggagagg agaatgtatt
    15061 cgttgccgga aatacaatta ctgccacagg taacttcgat gtaattacgg cttcatttga
    15121 cgcttccggg aatgagcttt tcaccacaac ttatgatcat ggtgaaaacg ggaatgacga
    15181 agcgaaaggc ttagcgattt tcgatgatgg atcatttgca gtttcatgca cattggcgcc
    15241 tgatactgat gcgatcacat taaatggaga cattgctgct gtatattttg atacaaatgg
    15301 gaactggcaa tctacaacca tatttgatgg cggtgcaaat gacgacgcat ctgaatcagc
    15361 atacgcttca aataccggat ttgtgatttg cggacaaacc gataacggtg aagcaggaaa
    15421 ccaagattat gattatatca ctatttatct tgatgaaacc ggcaccatta cgaatacaga
    15481 attatacaat ggtgcggcag gaggtaatga tgtagccaat accctgatgt tatatgacaa
    15541 tggaacctat ataagtggcg gaagtgaagg tggtcagcga gatatagtta ctatattata
    15601 cggtagcaat cctgtttcga tctccaacaa cgttgcatcc aaaaatgtgc aggtttatcc
    15661 aaatccaacc gttgcaacat gcactgttta cctcgatgaa atcaatctat catcttcagc
    15721 tactttaaaa ttatatggag gaaatggcaa ctgtgttatg caaaaagcaa tttcttcaaa
    15781 tgacaatata cagctaacac taagcaccat gccggcaggg atgtatctgt tagtgatcac
    15841 tgacggcgat caggtctatt ctaccaatct tattaaacaa tgatacttca ccttttaaaa
    15901 acagacttta tgcaagcgag aattaaaaaa tccattatct gcaaggaaat cacttcttat
    15961 cagatctaca gtacacgtaa caacacctac actccaattg atggcgatgt tgctttattt
    16021 gaggtactgg aaattggcaa acaccaatcc attcaatccg aaaccaagcg catagtatcc
    16081 atttttgaag gtgatgtgat catggcagca tttgcgaaca ggtatgctac atcacaattt
    16141 gaaggatatg ttcctgatcg acctacagaa attcttgaca ttttgggtgc cggtggtgct
    16201 atcggattgg tggcaagcaa aaatgctgct cttgaagata ttgagccgac aaaattgcgt
    16261 ttgattggat tcgccactac tacagaaggt caaatcatca ataccaagtt tttacataca
    16321 gcaagacaca catttaatgg atatgttccc aacaatgcca aaataatcct tagtgtaggc
    16381 tcttccatgg attcaggcaa aacgacatcc gcagcattta ctgcaagggg cttaaaaaca
    16441 acaggcaaaa aagtagcatt cattaaattg acaggcacca gttttaccaa agacaaagat
    16501 tttgtatttg attgtggcgc agatgtcact gtagatttca ccgattgcgg atatccatca
    16561 acatatatgt gtagtaagga ggaaatactt gatgtatatc aatcactgtt gatgcaactt
    16621 gagccacacg ctcctgatta tattgtaatg gaaattgcag atggattact cgaacgcgaa
    16681 actgatttct tgttgcgcga taaagccttc atgcaaacca tacacagtat gatattcagt
    16741 tgtggcgaca gccttggcgt attctatgga ttgcaattat tgcacagttt tggcattgtg
    16801 ccggctgtaa tttgtggccg ttttacgatg agtccgctgc tgattcaaga ggtgaaagca
    16861 aaaacaaata ttccaacttt agatatcgat caacttgcca atggtgagta cattcatgta
    16921 ttcgacaaat caggcatgca gattgttcat gctgcctaag gaaagtgaat attattaaaa
    16981 catattattc ccgccataaa gggttgctga tcagcgtcat tggtttaggg ttgttcagta
    17041 atgcgttgaa attggcaatc cctttatgcg tgggaaaatt ttatcagctt gcattggatg
    17101 gcggcagtgc caggagtgga atatttgacg cactgtttgg caatgtgcaa tcaatcaaca
    17161 ttttcttctc tatatttttt ggcatactgc tattaagatt tattgttcaa tttgcagaac
    17221 aatatttaat aggcatgacc ggcgaaacct tcagtgcctg gttacgcgaa acgctcttca
    17281 caaaacaatt acataccgtt tattcagtat ttacgcaaaa agatccggga aaatatctgc
    17341 ttcgctacag cggtgacatg tcggctctca gacttttcat tacaaaagga cagataggtg
    17401 ccataacaga ttttgcattc ctgataatga ccataggttt attcggttgg ttgaatatta
    17461 ctttaacata tttacttctg atagccattc cggcaatttt cctgataagc atactaatta
    17521 acagattcat gcgtccttct acagagaagc gtcgtgatat caatgcaacg aatttaaatt
    17581 tcgtgagcga gcggctgcat gccatggcaa caataaaggt gatgaatcgc agcacgcccg
    17641 aaatcaacaa gtttcaaagg agatcaacaa aacttttaga agcaggaaaa gtttatcagt
    17701 tttggaatgc attaaacaag gcactgtatc catttctact atatttattg ctggcgtttg
    17761 tcatgttgat gatctataaa ctgctaagca ataatactat tactgctgac ggcggaacgc
    17821 tactgatctt tgtcatgctc atcatccaga tcattcctgt tgtaaagaga cttttacgtt
    17881 caggtgctat acgcgaatca ggaaaactga gtttgaaaaa atgcaagctt ttacttgaag
    17941 cacctgaaga cagcagggaa ggtcatgcaa tgccggttaa aaaaggagat attcgtataa
    18001 acaacctaag cattaagttt ggtggtaaaa ccatttttga aaatgcaagt ttccatttcc
    18061 caccgaaagg aataacatta atttcaggaa caggtggaag cggaaaatca acgctgctga
    18121 aatacattag tggccttact attcctgagt caggagaaat tatgattgat gacgcatcta
    18181 ctaagtctta tagcatcttt caagtcagga agaaaatcac gtttatatct caagagcttc
    18241 ccttaatagg taaacatgta tatgatgcaa tttcctataa tcgggatcct gataaaaagc
    18301 atgatgctat gcaaatatta tcagcaatag ggttttgcga caaatttcag gatgaggttt
    18361 tggaactgcc gttggaaaaa ggcggcgcca atttttcaac gagtcagcaa caattattgc
    18421 gcatcgcacg cggactgatc acaggaaaaa aaataattct actggatgaa cccttcgaag
    18481 gattagatga tcgcagtgca cgtcaggtag catatcattt aacacatatc tcatcctcgc
    18541 acaccatcat tcttgtttca aaaagcatac ctgaaacact tttcatacaa caccggattt
    18601 cacttgacaa gttaaaacaa cccaatgtat aaattaacaa ttttagtaat cgggatcata
    18661 ctgataagtt cctgtacaaa ggatcccgga attggaggag aagccaccat taccgggaaa
    18721 gttcatgcct tgcattacaa cagcaccttt acggtattgc tttctgaata tgatcttgca
    18781 gacacgtatg tatatcttgt attcggcgat gatattaatt atggcacacg catcaaaaca
    18841 aactatgatg gtgaatatgc ctttgaatat ttatatcccg gagattacac catctacacc
    18901 tattctattg attcggcagc aacagtaaat ggaataccaa cgccgcctga ttctgcagtt
    18961 attcgacaaa tcaccatttc agataagaac gcattagttg aaacagaaga tcttgttgta
    19021 ttcaaataaa tatctatgaa aataatcatt gccggatgta ttatattaag tagcctccaa
    19081 ctattcggcc aaaatacccg cggagcaaaa accatgtatc aggctacacg agaaattgaa
    19141 gccggaaagc aggttacagt gaaacatgta tttcaatcat ttgataataa aggtcgcact
    19201 actgaggaat ggacattgaa aggtgatagc acttttgcaa gcagagatca atggacctac
    19261 aatcggaaag gcctggagac gaaacatata atatttaagg actcccttaa tattgaatct
    19321 atcatctcta catcatacga ccgctttgaa gtgaaaaaac tgtcaattac cacagatggc
    19381 aaaggttcaa taatcgagca aacggcctat cattataata ccttcggaaa aaaaatcgaa
    19441 gagttaacat cagattccgg gggaaatgtc accaaaaaaa taacattttc ctacgattca
    19501 catggcgcac ttgctgagag aataatatac gacgggaaaa ataatattgt aagcagtaaa
    19561 caatacaact acacataccg ataaaagtat gaaaaaaata ttgctttacc ttattgttat
    19621 gccgctatgc ttaaatgttc atgcacagca aagcgatgca gtatttactt ccggcatttc
    19681 tgttgaaaaa aagattacaa gaacatggtc ggcgagtacc tttattcaat ccggctttaa
    19741 tgaaaattac agtgaactcg gatatttgtt gatcgatggc ggactaggat tacgactgaa
    19801 tcgtgaatgg agccttgaag gtcattatcg ctacgggagt gttcgcaatg tagcgaatag
    19861 ttatgataca cgacaatata tttatggcga tgttcaatat gtaaaaggca ttggcgattt
    19921 tacattgata gccagatcaa ggtacatggt gaaaacttat ggcttgtata tttcagatga
    19981 aaatcaatat cgcgacaata agcacttcct aagaaacagg atccaattaa agtatgatct
    20041 tggtccttac agtgatattt tccttgcaac agagcaagta tacagacttg acgatcacaa
    20101 tgagtccgag caatttcgat tttccggggg atataattac accttcaatg tgcatcatcg
    20161 aatccagctg caatatactg ttgcgcagca ggtgaacaag aaagctcctg acacggacta
    20221 cattaccgga gtaacttatt attttaaatt ctaaatttgc ttcatgaagc attacatcac
    20281 tgcaatctgc ctgttggcag gtagtttgct ctttacctct tgcgataaat cttccaacgg
    20341 aggaaagagt gatacatcta cacctaaatt ttccatcgcc atacatggag gcgcaggtgc
    20401 tttggctaaa ggttcttata cagctgaaca ggaagctgca tttaaagcta aacttgaaga
    20461 agcaaccaag ctgggatatg aaatgttgtc gaaaggcgat agtgctgccg atgtagtggt
    20521 agccgttatc aagattttgg aagattcacc tttatttaat gcgggaaaag gctcagtaat
    20581 tacctataat ggtaaaataa aaatggatgc cgctatcatg aatggacgca ctcttgactg
    20641 cggcaccgta aataatgttc aacgcataaa gaacccgatc actgctgcta gaatggtgat
    20701 ggattcgagt aaatatatta tgttcagcag tgccggagca gaaagttttg ccgctaagaa
    20761 tggcattgaa atgattgata agtcctattt ctatacgcaa catcagattg atcgctggcg
    20821 caagacaaaa gactccactg atgcgtcgta tatacattat gtagattcca tggcagcact
    20881 gcaatcgcca attatttctg ctctgcccga ggagggggaa tttggaactg ttggttgtgt
    20941 ggtgatggac aaatatggca atctcgctgc aggaacgtcg accggtggac ttatcaataa
    21001 aaaatataat cgcgttggtg acagccctat aattggagca ggaacctatg ccaataataa
    21061 gacctgtgct atcagttgca ccggaacagg tgaagatttt atcaagaccg tagccgcaaa
    21121 aacagtgtct gacctaattg aatttaaagg tatgacatta gatgctgcaa catacgatat
    21181 gatacatgtt cggtttaaag agattaccgg agatggagga atgatagcgg tagacaaata
    21241 tggaaatatc tcatggcaat ataactctga tggtatgttt cgggcccgca cagatcagaa
    21301 cgggaaaatc gaaacctaca tttacgctga atagaaggca atcaatagtt actatgtatc
    21361 tttgtaaatg ctaatgcgta tacggagata tgtgcctgca aatcaactga tctacagttc
    21421 tctgggcctt gcctttattc tatgcatttt cgcctcctgt agtgacagca aagaccctgt
    21481 aattgcatat acaaaatcaa tgtatgtgct caacaaggac gaagaggaac ttcgctcggt
    21541 gaccgattat gattctatag atagaattat agcggaatat attattggtc gcaaaggaag
    21601 agacaccgtt gaaaaatcaa cctatgtata tggcgatgat ggattaatca ggaatacaac
    21661 cttttcgaat gataaaataa cacatcagca attttacgtg tatgccggaa gcatgctcat
    21721 gcaaatgatt gaaacagacg ggcatgatac aattgggata acagattatg tgtattaccc
    21781 taccggaaaa atcaaacgaa cggtgcttca actagtgtat gataaatctt ctcccacaat
    21841 tattgtaaca aattacaatc gacatggcga taaagaaacc atgtataagc agatttacga
    21901 agacagctct cgtctggtgc tttcgaggta cgagatggat aagtttataa gcacctatga
    21961 cagttccgaa aaaatgatca gaaatataga tcaattcttt ttgggctact atgaagaagc
    22021 agatacaaca cttgatgttt cctaccaata cgacgatgcc ggtcggttaa ttgtggaacg
    22081 caatgcgaaa cgaatagata cttccgttcc cgacagtata ttttatttct ataatgccgc
    22141 cggtttactt caggagaaac tcgtatttac aagtagtgca catcatcgtg agaaagtagt
    22201 aacaacagac agggttcaat atgactacga taaaaatggt cgccttatca aagaatatag
    22261 tttctattat aaaaccggaa ggcgctattc atatcgggca aaataaaaaa agccggatgc
    22321 gtgtgcacat ccggcttaca gtccaagcaa ttgcttacat ttttacaatc ctaaatgttt
    22381 gcatttgcga accatgattc accaccaaca aataatttcc ttgcggcaag tcctgaccaa
    22441 tttccacact accggttccc ggctcagcta tcacagtgct tactgtacga ccggtaatat
    22501 ccaaaacaac aatctccgtt tgcattgtga tattactgca atcaatcacc gcataatcag
    22561 taaatggatt gggagaaaca ttcacactta ccggtgcaat ttcaatttgt tcctcgcgac
    22621 aaataatcac actaacatta atcacattgg aagtttttga acagccattt gcattcgtaa
    22681 taactacttt ataaccacct gctgtagttg ctgcataagt agatgccgta gcacctgcaa
    22741 tattcgcgtt gccttttttc cattgataag ttaatcctgt tccagtattt gcactcaagg
    22801 ttacacttga tccactgcaa atagtggttg ctcccaaggc agtaatagaa gcggccggca
    22861 caggtgtgca atatttaatt gtagtaaaat caaaattatt tttccctgtt ccggtgccat
    22921 gaccagccac aaaaaggtat ccattattat ccaatttaac ctcctttgca acgtcttcac
    22981 catttcccgg accattatat tttaatgtaa gcagttttgc cccagcggga ttataatcta
    23041 aagtgataaa atcagataca gaggtggcat ttcctgaagg gcctgtagta taaatattat
    23101 ctacgccgtc agaagttacg cttacacctt catcggcaaa tgcaccggct ccagtatatc
    23161 gttttgccca gttttgagca ccggttgtct tgttgtattt aatcaaagct aaatccgctg
    23221 ctccggaagt tccaacactg cttcctgtta catatacttc agctcctgca actgtaatac
    23281 catttgccac atcaacacca tcaccggggc cattataatt cttttcccat tgcttaacaa
    23341 aggtactatt atattttatc gtcacataat catccatatt tgtatttaca aatgaagatc
    23401 ctgtcacata aacatcgctg ccgtcgatca gcatgtcttc agctgcatca tcaccattag
    23461 aagcgccatt gtaggtaacg gctgccaatt gagtgccgag tatatcatat ttaattagca
    23521 cgatatcatt gtttgcacca ttaaatgtgg tgcctgctac taatacattt gagccatcaa
    23581 acaatatttc tgccggttta tcagcagcat tgccaacacc attgtagcga gcagtccatt
    23641 gcaataatcc aagtgcattg atttttatag ttgtaatatc ataatttgtt acaagtgcac
    23701 ttgcatcgcc atctgaataa cctgttacat aaacgtctcc ggcatcagaa acaacaatgg
    23761 aaacagcctt gtcaagttga tgcgctgtat gatcgaaaat aactgtccac aatgctacac
    23821 ccagtgtgct atatttaatt acaatataat catcataggt tccacttcca ttgctataac
    23881 cggccagata agtatttccg gccgcatctg tagttactgc gagcgcttca tcattttcat
    23941 ttgttccatc aaatgttcta gtccagtttg tagttcctgc agggctaatt ttttggataa
    24001 gtatgttgcg ttgctgtccg gctgccatag tataaccaac aacgatacta tttccggatg
    24061 catcggtggt aagagcattc actttatcat taaaatcgcc cttgccattt attgtttttg
    24121 ttgcgcttaa aactccggct gctgtatatt ttaatgctgt gcaatctttt tgtgtagcag
    24181 taaaatttgc tgctccaaac acccagacat tagaagatgc atcggttagt actgtggcag
    24241 gaatatcttc ggcattagaa gttgtgccat caaaatatgc tgcccattgc tgagcgcctg
    24301 cactattata ttttatagtc atgaattcaa agttcacatt tgcaccacct atcgcatcgc
    24361 ttttacctgt cacataaata tttcctgatg catcaataaa catgtctgtt ggtgtatctt
    24421 cctgcaatac aggatttcct gaaagtgtat tccaaacaac aacgcctgtt ggatcatact
    24481 taatggttct aaaattataa tctgaatttc caccggaagg atcaatatca ctttgaccag
    24541 ttacgtaaac atttcctgct ccatccaatt ttaataaact cactcgatca ttatcaccgc
    24601 cattaaatac tttcgtccat tgttcaatac ctgaactgtt atattttaca gttaaatagt
    24661 cgtcatctga tgcgtttgca cgtaatccgg ccacataaac atttcctgca gcatcacgtg
    24721 ctatagactg acctctgtcg tcaccccccg gaccggcaaa aagtgccgac catgaaacgg
    24781 tacctaatga gttatagctt atagtaagca tatcgtcatt tgccccatta ttacttcttc
    24841 cggtaacaat acaaccacct gattggtcag caactatacc tgcggctctg tctgtaaatc
    24901 cgttatcgaa tcttgccgac catgataaaa cacctgcaga cgtatattta agtgtaataa
    24961 aatcatcaac attgagtgca tcatgatcac tcctgccacc aataaaaata ttgccggcgt
    25021 tatcaattgc aagtcctgcc ggtagttcat cttcattggt agttacctca ttaaaccttg
    25081 ctgtccacaa caaggtacct gttgcactat attttagtgt tagcacatcg gttttcgtcg
    25141 atgcagaacc gccatcggaa taaccacaaa ctacaatttc acctgcagga gttaaggcaa
    25201 tatagtttgc ttcgtcatcc aggttggagg tataattata ggtgcgtgtc caaaccgtat
    25261 caccggcact attcagcttt acggtataga agtcctttcc cttgcccgga ttcacggtat
    25321 atccggcaca ataaaagttc ccaagcccgt ctgcaatggc ctggttatat cggtcggaat
    25381 tatcgccggt gccattgaac ctgtttgtcc aaagagccga ttgaccggaa agggcggctg
    25441 aagcgaaggt tagtgccagc aggagcgtat tccggccggt tggtcttttt tttgtcattt
    25501 ggtagaattt tttcggtaat gaatcaaaaa ttggcgccca aatcaagctt tttggcctgt
    25561 ttgggttctg caaggtcaaa attgatgtct atacacagaa cccccaacga cagaaaacca
    25621 aggcttccat tgaaggtgta gaattacaga gcagaaaaga gccaaagaaa acacaaaacc
    25681 cttgtgacag tagggctgta gatgaaaaaa ggcaacgaca gaaattgatg ccttccgaaa
    25741 aatcagggca aaaaaaacct cccggtctta cgatcgggag gttttaagtt caagtatgaa
    25801 aagaaaagag aatactaaac tgcaaaactt tctccacatc cgcaagttct ggatgcattt
    25861 gggttaacaa aatgaaagcc tttgccgttc agcccatcag agaaatcgag ggttgtatcg
    25921 cacaggtaga ggaagctttt gaggtcagtg accagttttt caccgcgatc ttcaaaaatt
    25981 tgatcgccgg gtttttcttc gttgtcgaag tccattttat agctcaatcc ggagcaaccg
    26041 cccccaacaa ccgacacgcg aatgaagtaa tcgctgccaa ttcctgcctc agatttgagg
    26101 gattctaact tgtgtttagc cggttctgaa ataaaaatca taggttttgc atttaccagt
    26161 tacttaataa acgccttgat gatagcgctg gttcctttag tgaacgatct tttcatcaag
    26221 ttgcaattcg ggcatgccat ttttcttgcg ataatcatta atagccgctt taattgcatc
    26281 ttctgccaaa acggagcagt gaatttttac cggaggcaat gctagctctt caacaatatc
    26341 catattgtcg atcgtcagtg cttcatcaat tgtttttcct ttcaaccatt ctgtcgcaac
    26401 agagcttgag gcgatagcac ttccgcatcc aaatgtttta aatcgggcat cgcgaatcac
    26461 ctttgtttca ggatcaactt caatttgcaa acgcatcaca tcaccacatt caggagctcc
    26521 taccaaaccg gttcctactt cctgtttatt ggcatcgagt gttcccacgt tttgcgggtt
    26581 ctcgtaatgt tctaacactt tctttgagta tgccatttta ttatttttta agtatgccta
    26641 aattcaatat tataattaat gttctgccca ttcgatggtg cttaaatcaa ttccttgctt
    26701 aaacatttcc cacagaggac tcatttcgcg aagcttatta acagcttttg taactgcttc
    26761 aatcgtataa tctacttgtt catcggtgtt aaaccttccg agtgaaaaac gtatgctgct
    26821 gtgtgccagg tcatcaccta aacccaatgc tttcagcaca tagctaggtt ccagagatgc
    26881 gctggtacag gcacttccgg agctaaccgc aatattctga ttgaatgcca tcatcaaccc
    26941 ttcaccttct acatatttaa atgaaatatt ggtgatatgc ggtaaacgac tcttgcgatt
    27001 gccatttaca taagcctctt ccatctgcat taatgcattc tccaaacgat cacgcattac
    27061 gctcaatctt gcagcatctg catccatttc aagcatggca agttcagccg cttttccgaa
    27121 tcccacaatg ccgggaacgt ttaacgtacc actgcgcatt ccgcgttcgt gtccgccgcc
    27181 atccatttga gccgtaactt tcacccttgg attttttcta cgcacatata aagcaccaac
    27241 acccttcgga ccatacattt tatgtgccga aaaagccatc agatcgatac catcgcgttc
    27301 aacatctatt gaaatttttc cggctgcttg tgtagcatct gtaaataaca atgatccttt
    27361 tttatgtgcg atatcagcaa tctgacgaat aggtgctata gttccaattt cattgttcgc
    27421 atacatcacc gataccagaa ttgttttatc ggtaactaca ctttccaatt cattgagatc
    27481 aatatttcct tcgttgtcag ttttcagata agtaatttgt gcacctaatt tttcgaggtg
    27541 cttacaagta tctaaaactg ctttatgctc ggtggttaca gttacgatat ggtttccttt
    27601 tttaccatac atgtcaaata cacctttgat tgcaaggtta tctgattcag tagctccgga
    27661 agtcaggata atttctttat ccgatgcatt taccagtttg gcaacacgtt cccttgcctg
    27721 atcaacagca tcttccgcct gccagccaaa agggtgactg cggctggctg cattgccaaa
    27781 atgttcgtag aagtatggca ccatggtatc caccactcgg gggtctaccg gggttgttgc
    27841 gctattgtcg aggtatatcg ggagcttaag cataatttca ctatttagac tgattctaaa
    27901 acacaaaggt atataaaagg ttcgattcag cgcactggtt ttggaggatt ttaggagtgt
    27961 ttatgccctt ttttgcccat tttaaggcgc ggggtggcga aatgggggcg ttttggctgt
    28021 ttttacctct gaaaacagac tcgttgtggc atccgtcatt gcttaatatc cgtttaggct
    28081 tataccttta tcggacggac gccttaggaa gcaattaagc cttctgagta atcaacccaa
    28141 tccgtgctaa acatatcatc atgaaaaaaa tcgaacatat tggcattgcg ataaaggcac
    28201 tggaatcatc aaatgcatta tttgcaaaat tattcgggaa ggatcactac aaagttgaat
    28261 ctgtagagcg cgaaggcgtg agcacttcgt tttttatgat gggagaaacc aaaattgaat
    28321 tactggaagc cacaaatccg gaatctacca ttgcaaaatt tattgaaaaa cgcggagagg
    28381 gcattcacca tattgccttt gccgtagacg atatttatgc agaaatgcat agacttgaat
    28441 cagaaggttt tcaattattg cataaagaac cactgcccgg ggccgacaat aaattaattg
    28501 catttttgca tccaaaatct actaatggtg tgttggtaga aatttgtatg gagcgaaaat
    28561 agatgcatta ttattgccag atagcaatat ccttcatctc aaaccatttt tcatagttat
    28621 cctgaaattt cttttcaact tcattgcgtt taaccttcaa tgaaggcgta agcaagttat
    28681 ttgcgattgt ccattcttcg ttgagcacaa caaccttctc taatttttcg aaagaatcga
    28741 gggtggcgtt aatcgccttt aatgttttca ccaaatcatc cgacacttcc tgctttgatt
    28801 ttttctttcc gttttcagaa agtgtaacca atgcgatagg ttgcggcaat gcataaccta
    28861 caaccaatac ctgaccgaca tcagtgttat tggcaaattt catttcgata ggcattggtg
    28921 ctacgtattt gcccttgctg gttttgaaaa gttcttttac acgaccggta atcttcaaaa
    28981 atccatcggc gtcaacctca gctttatcgc cggttttaaa gaatccttct tcatcgaaaa
    29041 cctcttgagt gagcgcatct tctttataat atccgagcat catcgcggga tgttttacca
    29101 acatttcacc ttgctcacta aatttcaatt caacaccggg gaaacgcaat ccaactgtgc
    29161 caattctatt cctgtcttta taatttgt
//
LOCUS       DAOQOU010000022        28259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_761_length_28259_cov_6.454368, whole genome shotgun sequence.
ACCESSION   DAOQOU010000022 DAOQOU010000000
VERSION     DAOQOU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_761_length_28259_cov_6.454368"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..2449)
                     /locus_tag="PLM90_03440"
     CDS             complement(173..2449)
                     /locus_tag="PLM90_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ88452.1"
                     /translation="MRSWITLFTSLFIFSVAQASNPAADCVAAFEYEVSGATVYFIDG
                     STADPGPILEWFWDFGDGTTSTESNPVHTYAEPGEYDVCLTIHSDGGCFDDKCESNIP
                     VDGGGADCVVTASLVAIDGSSGHFIAEVTPAADIVTYTWNWGDGGPLYSETTTGAASD
                     PWHTYSDPGFYTVCVTITTGAGCTDEYCFEIEIPAAGDCYALYEYTLDGGLVHFYEAA
                     GGGDIVSYSWDFGDGSISTDPNPSHIWAAEGTYLVCLTIETATGCSDTYCHEITIGGG
                     GDCTSDFEFDLDGLTVHYFETAHAGGGGADIVSYYWDFGDGTSSDAPNPIHTYTEEGT
                     YLVCLTIVTNTGCTSTYCHEVHVELPTGDCLASYVVSSIELTPDGYVIHIENTSTSGG
                     DITTTTWYYGDGTIGDSYDAEHVYTESGTYIICLVIATADGCVDEYCTSVVVGEPGCS
                     SFFEFTTDGPAVHYFEGADGGGYDIVSYLWSFGDGTFSDGPNPIHIYTEAGTYEVCLT
                     ITTASGCVSAYCHIVIVEEGGEFCAAGFETDGIEETPDGWMAHFANTSIGSDLYHWTF
                     GDGGVSELPNPSHLFETAGIYAVCLTIGVGGGDCFDNYCEELFVGGDDDCVNAGIIDS
                     TMDCVEVYEPVCGCDNVTYDNSCFAQYYGGVVYWTEGACGTTAVQEENIFGSVQVSPN
                     PANQQLRVNYHLESSADVRIEMISLTGQSVIIPIYHQSVAGNYSVEINTSTLSEGIYF
                     IRLAAAGDAQMVKVIVTH"
     gene            complement(2461..3906)
                     /locus_tag="PLM90_03445"
     CDS             complement(2461..3906)
                     /locus_tag="PLM90_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88453.1"
                     /translation="MESSFFTLSKKTYGMIRLENPLNSALRAMMALSLVIGLSAAVDA
                     QTTTIAVNDSIKICEGTDVMIDVLLNDIDPDIEILETDIIAGPTSPLIDYDDDALPEG
                     SYNIYIDPTFVGEDYILYEVCGEDDLCSMGVIVIIVAGTDACVWPGDANQDNICNAAD
                     ILPIGLYYGLMGPDRDDDDGSWENTFADEWTDVPGAYYPNPKYSDINGDGIVDGNDTT
                     YLVANYGFISDVYTPYEAVGGADDPAISLGIFSDTIETGTAVTIPIYFGTDLIPASGI
                     YGIAFELSYDATLIDPSSVHVSFYDSWLGTMGDDMFALQHNDEATGVVEVAVTRNNQI
                     ARTGAGYFGQVSFVMEDNIAGKTTGQITSTASFCISKPNAVDGLGTITPVQISCDSVV
                     VIDFSNSIDNTSSRGIQVFPNPASAELTVTLPESEATIHLINALGQEIMTMQANNTQI
                     TIPVESIPAGTYMIQISGAHINHTQQCIIQH"
     gene            4127..5551
                     /locus_tag="PLM90_03450"
     CDS             4127..5551
                     /locus_tag="PLM90_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88454.1"
                     /translation="MIQMLKHFSTKELTRFGEYLESPFFNKDEQLSVFYQYVKKYAPD
                     FESRQIEKDRILQKGIPGIDINEKKLGYLMSDIVEHIEGFIRYNQHMADDMEGYVHLL
                     NMYNSWEADKFFEQTLREARTTLDADPFRNAPYFYKAFLLQSELNTFFDRQKKRAYDA
                     SLQEAANYLDLFYLSTKLKYSCELINRQKLVASDYTLRLLKEISDHLRDQSYDDYPSI
                     GIYYQILMTFVEGENEVHFEKLKLLLNEHTEKFPHNEARDMYAYAQNYTIRKINAGDK
                     KYLREYFDLSRSALEKGLLTVDGDLSPWTYKNIVVAALRVGEYEWAEQFIKQFKSRLN
                     EKFRSNAYSYNMGALMFYKGQYGEALRLVNQVEFTDIFYALDTRTMQIKIYYQLDEWD
                     PMQSAIEAFKVYLRRNKTLSDNVKQLYNNFLKFIEKLSKLTKRDKPKLQELMERIDET
                     RQVADIGWLQAKVQEKIGSERAAS"
     gene            complement(5723..7738)
                     /locus_tag="PLM90_03455"
     CDS             complement(5723..7738)
                     /locus_tag="PLM90_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ88455.1"
                     /translation="MKKLSTLLCALVLGTVLLRAQAPTQPNFVLIVADDLNDYVQGFS
                     GHPQAKTPNINKIEKKGTTFTSTYCAGPKCAPSRTSMITGKDTYYTKVYNNDDIGCKN
                     FRKNFREEKGNEVVFTIPELLKDSGGYFTYTLSKVMHCYPGLPDYDTSNVAMCDKGLS
                     WNKAFSYGQGGDMTDVLDYGDEHDDGVTGFNFAVIPDTMEDNTVDYIITDSAISFIED
                     YASNPATYCNKPFFLAIGYKRPHGPFYVPEKYFLPYYNTDYYSVPYDKPYNDPVGATP
                     YNGVVMPPQPDPMWSDYTSLPDDLVGKAMNEEDYVHPDFLEFGDYMMGLIPPPEIAAG
                     LSDSEKTFILQESERANGIIAYLAAIKFFDAQVGRFYDEIKSHPEIFNNTVFIITSDH
                     GYSLGEKTHWRKGCLWETDVRIPMVIADMRTPVKKTCKRVVGNIDLMPTILEMAGVDA
                     PTFPDGSPYMDGVSLTPLLANPNTPWDKPVVSALQLHGYAEGFCFPQYSVRDEEYHYI
                     RYQTNGATAPACNEALSVFQEELYRIGKNKNIDPYEWNNLADNPAYDDVKAHLASFLP
                     GGVNYLQFDRTASADDAAMAVQAFGVFPNPTSEYANFRMEGFTPGAATLQITDLMGRI
                     IRNDEVTVDENGYLEVSYNVTTIQQGYYIGRVIQGNDSMQTEFIVAR"
     gene            complement(7801..9810)
                     /locus_tag="PLM90_03460"
     CDS             complement(7801..9810)
                     /locus_tag="PLM90_03460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88456.1"
                     /translation="MIKLSIIIVNYNVKYFLEQAVLSAIKACANIPAEIIIVDNNSSD
                     GSVSFIHQKFIDLQNSHTPVHLIANQENTGFSKANNQGIAIAKGEYILLLNPDTVVQE
                     DTFEKCLAYMDAHPKAGGMGVKMIDGKGAFLAESKRAFPSPEVAFYKAFGLANLFPDS
                     KVFGKYHLGYLSENENHEVDVLAGAYMLIRKRVLEEIGYLDETFFMYGEDIDLSYRIV
                     KKGYTNIYFADTSIIHYKGESTKKASFNYVKMFYTAMKIFAQKHFSGPRAGLFVLFLN
                     TAIYFRAGIAALMRMLGKMASPLFDGALLFSGLFLVKLYWEYYVKYIEGGTYPDAYLY
                     INVPAYMLIWLFTLFLSGGYDNNATPGRIVRGMVWGTFIIAAVYGFLPEHLRFSRGMI
                     IAGAALNIALLIGARGFLHFIKYGNIRFGEAPDKRILLVGGVKEITRAQQLLNTLQLG
                     NQLIGFAGNNKNIEHPLLLGNIDELPLIIRNFRINEVIFCADDVPATEIVRLMTLCGK
                     LTDFKIIPPGSDSIIGSNSKNTAGDIYTMEVQMNIHHPQKQRSKRLLDILIATFGIVL
                     SPLLIWFMDNKAGFLNNCFQVLWGRRSWVGYISSSSGISSNGQKLPTLLPGVLHPLSA
                     GTTVTIAPHIAERVNFMYARDYTPAADLMIIKGAWRRLGGMETVM"
     gene            complement(9807..10436)
                     /gene="recR"
                     /locus_tag="PLM90_03465"
     CDS             complement(9807..10436)
                     /gene="recR"
                     /locus_tag="PLM90_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMZ88457.1"
                     /translation="MNLPSQWMENAVNEFAKLPGIGKKTALRLVLHMMKQPQDQVAAF
                     GAAMLKLKTDIHYCPKCHNLSDHPGAECSICSGPRRDHHTICVVESIRDLIAIESTNQ
                     YHGEYHILGGVISPIEGMGPEQLFIDDLIAKVNSGTCNEVIMALNPTIEGDTTVYYIT
                     KKITKQNVRITSIARGISFGGELEYTDELTLARSLATRRPYDNYMQQSE"
     gene            complement(10470..11186)
                     /locus_tag="PLM90_03470"
     CDS             complement(10470..11186)
                     /locus_tag="PLM90_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HMZ88458.1"
                     /translation="MRSKKVTLTLALFGGSTGADRFYLGQNTAGWLTILLCWLVLPGA
                     VYGIMRYNLYPNWEPFLLARYALPILFHVFATGRYLIMSEESFMSQNVKKSNTFPMVL
                     LSFVITAILIVGSSRLLQTVQIVDIDKAEVEAVLSAEMMSQEFRNDEEAYRKEYDNKT
                     LQIEGVVVETGNDFELGTYFALRGKDGDPFGIKCYFLGDHLKEAEQVVMGDKVTMKGI
                     ANGNKLENCTLLERNGEKVK"
     gene            11362..12279
                     /locus_tag="PLM90_03475"
     CDS             11362..12279
                     /locus_tag="PLM90_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ88459.1"
                     /translation="MKRALYIVLLSFIPLGTQAQQYLEIGAFGGVSNYQGDLAPVAFV
                     PTETHLSYGAFYKFNLNYWFGLKGSLYSGFISGNDDNSTEEWKQERNLSFVSPVQEAS
                     AVFEVFLKRYNPKYKWNIMSPYIYFGIAIFHFNPMAQYQGVWYDLQPLGTEGQGNPAY
                     PDRKLYPLTQYSYPVGFGIKGSISPEWNVGVEIGYRKTTTDYLDDVSTTYIAKEILLA
                     DNGDIAWQLSNRTDEMNGGFEVLRDDKKYRGDDTNKDWYIFTGVTISRNYEVKGWESR
                     KYKASDNPKKPGLRKSRKKMDCPGVNLKL"
     gene            12406..13308
                     /locus_tag="PLM90_03480"
     CDS             12406..13308
                     /locus_tag="PLM90_03480"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ88460.1"
                     /translation="MKVLQLLGLLTLSGSMAAQMSYFEVGVMGGVANYYGDMTHDYVV
                     ADESHFAYGGYIKYNLDHKMGLKFNVYSGTVSAADANSDRANLKARNLSFTSPVTEAA
                     FTFEYNFFGYRPVEFKQRYSPYVYAGVAAFHFNPQAYYEGEWYDLQPLGTEGQGSEYF
                     PYRDKYRLYEFAIPFGVGFKWALTERWNMGLEYGARWTFTDYLDDVSRTYVDRNLLIE
                     SNGIDAYNLSNRSGEYLGGEPFDYGNNDWRGDPTNTDWYMWLGITFSRNLIKGLDEGF
                     RTSAKDQLGCPGPRHINKVKKKKT"
     gene            13428..>13706
                     /locus_tag="PLM90_03485"
     CDS             13428..>13706
                     /locus_tag="PLM90_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ88461.1"
                     /translation="MAGWLFLCVLLAYFGLLFTISWFTGRKATDSAYYLGNKSSPWYA
                     VAFGLIGDSLSGVTFVSVPGSVATQQFGYMQMVLGYLVGYYVIAKVLLP"
     assembly_gap    13707..13806
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13807..14757
                     /locus_tag="PLM90_03490"
     CDS             <13807..14757
                     /locus_tag="PLM90_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ88462.1"
                     /translation="IKTLVWTDTLQSSFLLAGVLLSIVVIAGALHLDAGGIVQTVRDS
                     NYNNIFFWDPMKQNYFFKQFISGALIAIVMTGLDQNMMQKNLSMRTLPEAQKNIMWFS
                     FVLIFINLFFVSLGSLLYAYADSIQLTLPARTDHLFPMLALDHLGVFAAIVFILGISA
                     ATFSSADSVLTTLTTSFCIDVLKWDPNGNESGKHRRQRHLIHVLFAVLLLIVIIVFNA
                     LNNDAVINQVFVAAGYTYGPLLGLFAFGILTKRSLNAKAVLLICLLPPVACYYLNENS
                     VTWFNGYQIGNELLLINGILTFVGLYVISKAPQQNNIQTA"
     gene            14767..15474
                     /locus_tag="PLM90_03495"
     CDS             14767..15474
                     /locus_tag="PLM90_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ88463.1"
                     /translation="MAEKYRKKKLFKTKDWTEISAQSSWRMFKIMAEFVDGFETMDKI
                     GPCVSIYGSARTKPDHKFYKLSEQIAARLVGEGFGIITGGGPGIMEAGNKGAYKEGGK
                     SVGLNIELPYEQSHNPYIDRDKLINFKYFFVRKVMLVKYAQAFIFMPGGFGTLDELFE
                     ALTLIQTHKIERVPVIMVGKTYWKGLLAWMRETMLHKEHNISEEDLDLFFVSDEPEEI
                     VQHINAFYATSHLRPNF"
     gene            15474..15947
                     /gene="bcp"
                     /locus_tag="PLM90_03500"
     CDS             15474..15947
                     /gene="bcp"
                     /locus_tag="PLM90_03500"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HMZ88464.1"
                     /translation="MKEIITLQPGDKVPAFSASDQYGNKITAASMKGKWYVLYFYPND
                     DTETCTKEACNLRDNHAALLKAGITVIGVSHAPVESKLKFTKKYNLPFILLADTDLKI
                     CKSFGVYGDKLFMGKVITTIHRITFLINDKGRIEHVISNVKSGQAASQILDLMKK"
     gene            16002..16841
                     /locus_tag="PLM90_03505"
     CDS             16002..16841
                     /locus_tag="PLM90_03505"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88465.1"
                     /translation="MFSARQFPTKLISAAFGVLIIMGVISISFAKNDGAPSGNTGSPG
                     DDQTCAHTMCHSGTASEQIGLISTNVPASGYLAGETYLVTVTVTEADIVKFGFQASPQ
                     NLDGDRMGDMSVIDATETKLTGGGKYITHKSTGTAGSGSRTWTFNWTPDESTGEVGFY
                     VAVNASNNEDDASGDHIYYDAIFIPEDPDNVPVKVDDIATIDCTVNAISNDMIYVQYQ
                     ASGVHAVKAQIFDMMGRELLDEENDTQASSFTLDTSGLSAGCYLVVLTSGTARFVQQI
                     VLP"
     gene            complement(16894..17340)
                     /locus_tag="PLM90_03510"
     CDS             complement(16894..17340)
                     /locus_tag="PLM90_03510"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HMZ88466.1"
                     /translation="MRFREITAITGLGGLYKMESQKASGLIVTSLAEGWTKFISNREH
                     LFSPLENIAIYTSDDTVELLEVMLKAYEQKDSNPPVDAKADNGTLSAYFEKVLPEYDR
                     EKVHISDIKKFIKWFAILDEKGVLKTELEEKAKEAEAEKATDEKSE"
     gene            17440..19461
                     /gene="uvrB"
                     /locus_tag="PLM90_03515"
     CDS             17440..19461
                     /gene="uvrB"
                     /locus_tag="PLM90_03515"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMZ88467.1"
                     /translation="MPFELISQYSPTGDQPGAIKQLTEGLLAGEPSQVLLGVTGSGKT
                     FTIANVIQNLQKPTLILSHNKTLVAQLYGEFKQFFPNNAVEYFVSYYDYYQPEAYIAT
                     TDTYIEKDQSINAEVEKLRLKTTTSLQSGRRDVIVVATVSCIYGMGNPAEFKDSIVRA
                     NVGDKMSRNYFLHRLVDALYSRAAVDFKRGNFRVNGDVVDVFPAYADIGYRITFFGDE
                     VEEIETFNPENGKKLERIQNIAIFPANLYIAPKDILPSIITDIYAELDAQVKYFESIG
                     KYIEAKRLSERVNFDIEMIKELGYCNGIENYSRFFDRRTKGQRPFCLIDYFPDDYLLV
                     IDESHATVPQIGAMSGGDRARKMNLVEYGFRLPSALDNRPQSFDEFMTLLNQVIYVSA
                     TPGDFELNQTGGVVVDQVVRPTGLLDPPIEIRPTLNQVDDLLHEIDERIKKDERILVT
                     TLTKRMAEELSKYMLKMGVRCKYIHSEVETLERVEILRDLRIGRIDVVVGVNLLREGL
                     DLPEVSLVAIMDADKEGFLRNTRSLTQTAGRAARNANGMVIMYADKITDSMQRTINET
                     TRRRQRQMEYNAEHGITPKTVIKSTDEIFTIGKDNKKSVQAYIEPQDVSLSLVADPVV
                     HMMGKPQLEKAIADTKKKMLAAAKDMDFLEAARLRDEMYALEKLLDEKS"
     gene            complement(19462..21141)
                     /locus_tag="PLM90_03520"
     CDS             complement(19462..21141)
                     /locus_tag="PLM90_03520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ88468.1"
                     /translation="MRRRNFIRNLGMASPAVVFGPSFMEWHKWLPKEAGVGSVLIIGA
                     GAAGLYAARALQDAGVDVTILEASDTHGGRVRPLPGFADFPTEAGAEFVHGKGNVTGT
                     PPSFLWSSINDYNPDLLLEYGSHKEMYDIGGVIKTEPYWDADLETCWQFYLNMYAYTG
                     DEMLMSEYIDTEYGITPAHPYWHFYEAWIGSEFGTSVDRIGMKSIAISELLWLTGGKD
                     YLLDTSYRDLLDTLFFAPILDKVQYNQVVTGIDYFFPGVTVTVAGGATFSADKCIIAV
                     PLPILKNAEIIFNPPLPMQNQTAIDTLDMGAGMKIFLKFSTNFWGGDIGDITVDGYST
                     FLWAPGIDKTGATNNVLICFIMGENAEYMSALDAGAIDVALAELDALFDGAATANYVD
                     GFIQDWSKEPHIKGAYSFPAPNTYTTETDTTRLDLATPVDCKLFFAGEATNNNHPATV
                     HGALESGARAAAEILACGFSTVHTPETAFAQAYAHDNTAFFAITGNTSGTWSLLLTSI
                     DGKTVHTFMENSTIQGAQTFQFPLGNIASGNYMLTAMQNGKVRCSVMINKS"
     gene            complement(21830..22204)
                     /locus_tag="PLM90_03525"
     CDS             complement(21830..22204)
                     /locus_tag="PLM90_03525"
                     /inference="COORDINATES: protein motif:HMM:NF021525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HMZ88469.1"
                     /translation="MHKKIALDHIQNNSLTGIKAGVDRPGFLDIWMVVVEDRIFARSW
                     GFAERSWYNTFLQNPHGQIKCGNNIIAIKAVVPADIASMTEKINLAYLTKYNTGANSK
                     YAQGIVGEDHIAKTMEFVIVEA"
     gene            complement(22470..22850)
                     /locus_tag="PLM90_03530"
     CDS             complement(22470..22850)
                     /locus_tag="PLM90_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88470.1"
                     /translation="MNILSKFTFTLLLLFLHFAVHCQTRQDTLNKIQEYYNFALGKTV
                     AELLSKYNLDTTSNFEADEKLVYFSFDLGFYCCPRIACKNNNYGYWKQLLNQPIVYVE
                     TYNENGILKKIKTKGIRKKDYLPE"
     gene            complement(23013..23192)
                     /locus_tag="PLM90_03535"
     CDS             complement(23013..23192)
                     /locus_tag="PLM90_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88471.1"
                     /translation="MGTTGADEYMIAATFTLTGMPNINNVHFDFEPGDHASPGTYNRK
                     YYLDCIRENKEINKQ"
     gene            complement(23313..23612)
                     /locus_tag="PLM90_03540"
     CDS             complement(23313..23612)
                     /locus_tag="PLM90_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88472.1"
                     /translation="MSVPLQEDRFALRNPLLRQAPKRYQILLFACGRHVNSETDMLKS
                     DEHAGVSKDLKNQPDSLSLVQTNQGNKGDNISIWRYDYMADTIIQLRKIKIDTLT"
     gene            complement(23693..24613)
                     /locus_tag="PLM90_03545"
     CDS             complement(23693..24613)
                     /locus_tag="PLM90_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88473.1"
                     /translation="MKNKFTFPAYAAMATSFLTINAKVEAKAVYTDIDPDSILTIHEG
                     MEIDFNEDGVWDVFFMLSSWDGTTTYSMHFQAHDIHGGGNWPYGYLGKFSYFYAGSFL
                     GSQALLLPFIAGEEINDSFLFAGGELMYHFSQLSSPFIEWSGGYWYPEFLDGFIGVKL
                     KYFEGGEFKTHYGWIRCSVLDGGNELVIKDYAYELLPDVPIIAGDTVGRIVEFAHDTL
                     PHFGTGLPDADNEIVNASIYSFGSVVYISVPEFLENISLNIYNITGEIVYNRTMNSGF
                     LTVEMTQPSGIYLVELIADIQRTCRKIVID"
     gene            complement(24684..25574)
                     /locus_tag="PLM90_03550"
     CDS             complement(24684..25574)
                     /locus_tag="PLM90_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88474.1"
                     /translation="MANKFSLLQYSTAAAALLGVTAASSQVVYTDIDPDVVLDEPNEM
                     FGIDLDDDGLNDFNFFNESFITTVFYGDLANVKALFVGAFDTILNGIAGSTGFLSGGG
                     GYTYYWPYAMQNGVIIDENLNFFNSNYQSLVALKYKIDVPFVLPQGNWTSFYSGYEHL
                     NEYVAIRFADEEAIMRYGWIRCSVVDSGRTLIIHDYAYELQPDQPIIAGDTVSFVNIQ
                     EQPSLFATVYNYNTTIYINLHNYTPTQLTIVNLTGQTILDKSIYNQFEIVDMNYFPKG
                     IYLVTLKEGENILTRKVAIE"
     gene            complement(25577..28045)
                     /locus_tag="PLM90_03555"
     CDS             complement(25577..28045)
                     /locus_tag="PLM90_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88475.1"
                     /translation="MKNLIIKQRMLVLSIYIISFVFCNNKIYCQDIDTLEWEEIVDLA
                     DTVYNSGMDTIYVIEDSTSETTGFRTGASLDCATPASTMSINLNKTVKTINKGQFGVN
                     VTGMFTKSTLYGDIYSEDQWQWLSDMRPKVLRFPGGSSSKFMHLLTGDKGYGYDIVEI
                     TRYLDAIDNDLEAPTNATDIMNETNVTTLLNWFGGSSEILDAFEEYRTDYGYQEDLTS
                     GDRFIDHFIRLIQKIETDNQVFDPTYKVDVIVCLNILTETAEDCLDIVQYLQSNPIHN
                     VNVVGVEMGNETANKFHKQIMQFDEFENYWQYLDGQAVVGQASKEAAIGESLFIPAAN
                     RNFFYVFRNRSGVNYKIGLCAEGLNGTGEVFNILPAEDAGSRSVDWNVALRSHYGDTH
                     AGTSVKKFHAVILHTYFSADSWYNECVTETGILGSYSCPEWDFDNADTRIQPAFDAAR
                     LNFRKFMLTRFDNDLDLFNLDLNFNLTSSIKKDMWITEWNLKDEGTPNNGKIFTNSYQ
                     HTVLLQEWWLKNLKLNFTEGYRENFFKYATLQNYAGGSGVQMLTPADEDVELTLLGQD
                     VSPYNLAAGDPAKRNYYVKRTVGYTMDLISEINKNNLNYFPATVATYGHNPNLPPTFF
                     ITPAKDFVYMYFTNSRCNDQRYVLNPAGMYLMYGYQVELVDAEMFVVDAFQAYSQSGK
                     SMIYDFNSCYTPANPYDMEIDQTYNYVNPVCTAADGSLCITVPGFTSGYVKMAVVPYV
                     PRMADEINTSLSIYPNPANNLINISSTEQIVSVAVISMTGAKMFETIDNCNIIDVSML
                     SYGMYQVICTFSDGSNVTKSFIKL"
BASE COUNT         8283 a         5704 c         5786 g         8386 t
ORIGIN      
        1 ttttattccg cagtgatatt tgcacccttt ctatgtttaa tggaaaggcc agtagtgagg
       61 cgtattcgcg ctaagacagc aaagaggtga aggcgcaaag tttactgcac tcgcacacaa
      121 aaaaagccat cacctttcgg caacggcttt tgttaaccat tagggttact aattaatgtg
      181 tgacgatcac tttcaccatt tgagcatcac ctgcagcagc taagcggata aaatagatgc
      241 cttcggataa tgttgatgtg ttgatctcaa cagaataatt tcctgcaact gactgatgat
      301 atattggaat aataactgat tgacctgtca gagaaatcat ttcaatacga acatctgcac
      361 ttgattcgag atgataattt acacgcagtt gttggtttgc agggtttggt gatacctgaa
      421 cacttccgaa aatattttct tcctgtactg cagtagtgcc gcatgcacct tctgtccaat
      481 aaacaacacc accataatat tgtgcaaagc aactattgtc gtatgtaaca ttgtcgcaac
      541 cgcaaaccgg ttcgtaaact tcaacgcaat ccatagtaga atctatgatg cctgcattca
      601 cgcaatcatc atcgccgcca acaaacaatt cttcacaata attatcgaag caatcgccac
      661 caccaacacc aattgttaag catacagcgt agatgcctgc agtttcaaat aagtgactag
      721 gatttggcaa ttcagaaaca ccgccatcgc caaacgtcca atggtatagg tctgaaccaa
      781 tggaagtatt ggcgaaatgg gccatccatc catccggtgt ttcttcaatg ccatcagttt
      841 cgaatcctgc cgcgcagaat tcaccacctt cttctacaat tacaatatgg caataagcac
      901 ttacgcatcc acttgcagta gtgatggtta aacaaacttc ataagtacct gcttcggtat
      961 aaatgtgaat cggatttggt ccatcgctaa aagtgccatc gccaaacgac cataaataac
     1021 taacaatatc atatccacca ccatcggcac cttcaaagta atgtacggca ggtccgtcag
     1081 ttgtaaattc aaagaagctg ctgcatcccg gttcgccaac aactacagat gtgcagtact
     1141 catcaacaca tccatctgca gttgctatca ccaggcaaat aatatacgta ccactttcgg
     1201 tatacacatg ctctgcatcg tagctatcgc caatagtgcc atcaccataa taccaggttg
     1261 tagttgtaat atcacctcct gatgtagaag tattttcgat atgtattaca taaccatcag
     1321 gtgtaagttc aattgatgaa acaacgtagg atgcaagaca atcaccggta ggtagttcaa
     1381 catgtacttc atgacaatat gtacttgtgc aaccggtgtt tgttacaatg gtaaggcaaa
     1441 ctaaataagt tccttcttcg gtgtatgtgt gaattggatt tggtgcatct gatgaagtgc
     1501 catcaccaaa atcccaataa taagaaacga tatctgcacc accaccgcct gcgtgcgcag
     1561 tttcaaaata atgaactgtt aatccatcga ggtcgaattc aaaatcggaa gtgcaatcac
     1621 caccaccacc aatagtaatt tcatggcaat aggtgtcgct gcatccggtt gctgtttcaa
     1681 tggtaaggca aacaaggtaa gttccttcag ctgcccaaat atgagatggg tttggatctg
     1741 tgcttatgct gccatcaccg aaatcccagg aataagaaac aatatcgcca ccacctgcag
     1801 cttcataaaa atgcactaaa ccaccatcta gggtatattc ataaagagca tagcaatcgc
     1861 cggcggcagg tatttcaatt tcaaaacaat attcatctgt acatccggca ccggtagtaa
     1921 tggtaacgca aactgtatag aagcccggat ctgaataggt atgccaaggg tctgatgccg
     1981 cacctgttgt tgtttcgcta tatagtggtc cgccatcacc ccagttccag gtgtaggtaa
     2041 ctatatcggc tgcaggcgtt acttctgcaa taaaatgtcc tgatgaacca tcaattgcga
     2101 ccaacgatgc ggtcacaaca caatcagcgc caccgccatc aacaggaata ttgctttcgc
     2161 atttatcatc aaagcaacct ccatcggagt gaatggttaa acaaacatca tattcaccgg
     2221 gttcggcata agtgtgcacg gggtttgatt ccgtagatgt ggtgccatcg ccaaaatccc
     2281 aaaaccattc taagatcgga ccgggatctg ctgttgagcc atcaataaaa taaacagtgg
     2341 cgccgcttac ttcatattca aaggctgcaa cacaatctgc agccggattt gatgcttgcg
     2401 caactgaaaa aataaataaa cttgtgaata gtgttatcca acttctcatg gtattaattt
     2461 ttaatgttga atgatacatt gttgtgtgtg attaatatgt gctcctgaaa tttgaatcat
     2521 ataagtacct gcaggaattg attctacagg aattgtaatc tgtgtgttgt tagcttgcat
     2581 agtcataatt tcctgaccaa gtgcattgat taaatgtatg gttgcctcgc tttcaggaag
     2641 tgtaaccgtt aattcagcac ttgccgggtt tggaaaaact tgaataccac gcgaacttgt
     2701 attatcaatg ctgttgctga aatcaataac tacaaccgag tcgcaactga tctgcaccgg
     2761 tgtaatggtt ccgagtccgt caacggcgtt tggtttgctg atacagaagc ttgctgttga
     2821 tgttatttga cctgttgttt ttccggcaat attatcttcc atcacaaaac ttacctgacc
     2881 aaaatatccg gccccggttc tggcaatttg attattgcgt gttacggcta cttctacaac
     2941 tcctgtagcc tcatcgttat gttgcaatgc aaacatgtca tcacccattg ttcccagcca
     3001 actgtcatag aatgatacat gcacgcttga tggatctatt aatgtagcgt cataagataa
     3061 ttcgaatgcg ataccataaa tgccggaagc aggaattaaa tcagttccga aatatatcgg
     3121 aatggtaact gctgttcctg tttctattgt atcgctgaaa ataccaagtg atattgccgg
     3181 gtcatcagca ccgccgacag cttcatacgg cgtatataca tcgctgatga aaccgtaatt
     3241 ggcaacgagg taagtagtgt cattgccatc aacaatacca tcaccattga tatctgaata
     3301 ttttggattg ggataataag ctcccggcac atctgtccac tcatctgcaa atgtattttc
     3361 ccatgaacca tcatcatcat cgcggtcagg tcccatcaac ccgtaataca aaccgattgg
     3421 taatatatct gcagcattac aaatattatc ctgatttgca tctccgggcc aaacacatgc
     3481 atctgttcct gctactatga taactattac acccatgctg caaaggtcat cttcaccgca
     3541 tacctcatac agaatataat cttcaccaac aaaagtggga tcaatgtaaa tattataact
     3601 gccttcagga agtgcatcat catcatagtc gatcaacggc gaggtaggtc ccgcaataat
     3661 atctgtttca aggatctcaa tatccggatc aatatcattc aacagcacat cgatcatcac
     3721 gtcggttccc tcacaaatct taatgctgtc attaacagct atcgtagtgg tttgagcatc
     3781 cactgcagca gacagcccta ttaccaggga taaagccatc attgctcgca aagctgaatt
     3841 caaagggttt tccaatctaa tcatgccata ggtctttttc gacaatgtaa agaaactact
     3901 ttccataggc taaaaaaagg acatctttct ccaatttcca cgttttccga agcatttttt
     3961 tgccatttta ttattcaatt attattgaaa aaattgaaaa taattgaaaa tcaataaatt
     4021 gtgaatttgt ttgttgattt ggttatgcac cttacatatc gcatttccga caaaactcgt
     4081 atctttcggg gcttgtaaag agcgcctgag gatgtacaag agcaagatga tacagatgct
     4141 gaaacatttc agcacaaagg agttgaccag gtttggagag tatctggaat cgccattctt
     4201 caataaggat gagcaattga gtgtgtttta tcagtatgta aagaagtacg ccccggattt
     4261 tgaatcgcga caaatagaaa aagaccgcat cttacagaag ggtattcccg gcattgatat
     4321 caatgagaag aaactgggtt atctgatgag cgatattgtg gagcatattg aaggcttcat
     4381 cagatataat cagcacatgg cagacgacat ggagggctat gtgcatttat tgaatatgta
     4441 taacagctgg gaagctgata aattttttga gcagacgtta cgagaagcaa gaacaaccct
     4501 ggatgctgat ccgtttcgaa atgcaccgta tttctacaaa gcatttttat tacaaagtga
     4561 attaaatact tttttcgaca ggcagaaaaa aagagcctat gacgccagtc ttcaggaagc
     4621 agcaaactac ctcgatctgt tttatttatc cacaaaactt aaatattcct gcgagctcat
     4681 caaccgtcag aaattagtgg cgtcggatta taccttgcgt ttgttaaaag aaatttcaga
     4741 tcacctgcgg gatcagtctt atgatgatta tccgtcaatt ggtatttact atcagatatt
     4801 gatgacattc gttgaaggtg aaaatgaagt gcatttcgaa aaactcaaat tgttgctgaa
     4861 cgagcatacc gaaaaattcc cgcacaacga agcgcgcgat atgtatgcct atgcccagaa
     4921 ctacaccata cggaaaataa atgcaggcga taagaaatat ttgcgtgagt attttgatct
     4981 cagtcgctcc gcactcgaaa aaggattgct gactgttgac ggggatcttt caccatggac
     5041 gtataaaaac attgtagtgg ctgctttgcg tgtaggagaa tatgaatggg ccgagcaatt
     5101 tatcaagcaa ttcaaatcgc gcctcaacga aaaattccgc tctaacgcct acagctacaa
     5161 tatgggtgcc ttgatgtttt acaagggaca atatggtgaa gctttgcgcc tggtaaatca
     5221 ggttgaattc accgatattt tttatgcgct cgatacacgc accatgcaga taaaaatata
     5281 ttatcaactc gatgaatggg atcctatgca aagcgctatc gaggcattta aagtatatct
     5341 ccgtagaaac aaaacactct ccgacaacgt aaagcaactt tataacaact tcctgaaatt
     5401 tattgaaaag ctgtcgaaac tcactaagcg cgacaagccg aaactgcagg aattgatgga
     5461 gcgtattgat gaaacaagac aggtggctga tattggctgg cttcaggcca aagtacagga
     5521 aaaaattggc agtgaacgcg ctgcatcata aagtctgctt tctttacgaa tttgaatcgt
     5581 tagaattatt gactaattaa aaaattcccc tcgattctta tcctatttaa tttttgccga
     5641 accgcaattc ataatttact ataaataaaa agacccggat gaaaactcga tccgggtctt
     5701 ttgttttatt taattgctga aattatcgtg caacgataaa ttctgtttgc atgctgtcat
     5761 taccttgaat aactctacca atgtaatatc cttgttggat agttgttaca ttatatgata
     5821 cttccaagta accattttca tcaacagtaa cctcatcgtt acggatgatg cgtcccatca
     5881 aatcagtgat ctgtaaagtt gctgcaccgg gtgtaaatcc ttccatacgg aaattggcat
     5941 attcacttgt tggatttgga aaaacgccaa atgcctgaac agccatagca gcatcatctg
     6001 cggaagcggt gcgatcgaat tgcaagtaat ttactccacc cggtaggaaa ctagccagat
     6061 gtgctttcac atcatcgtaa gcagggttat ctgccaggtt attccattcg tacgggtcga
     6121 tgtttttatt ttttccgatg cgatataatt cttcctgaaa aacacttaat gcttcgttac
     6181 aagcaggagc ggtagcacca tttgtttgat aacggatgta gtgatattct tcatcgcgca
     6241 cgctgtattg agggaagcaa aatccttcgg cataaccatg taattgcaat gcagatacaa
     6301 ccggtttatc ccaaggtgta tttggattgg caaggagtgg tgttaagctt acgccatcca
     6361 tgtatggtga accatcagga aaagtaggtg catctacgcc ggccatttca agaatagtag
     6421 gcatcaaatc aatattgcca actacacgtt tacatgtttt tttaaccgga gtacgcatat
     6481 ctgcaattac cataggaatg cgtacatccg tttcccacaa acaacctttg cgccagtgtg
     6541 ttttttcacc taatgaataa ccatggtcgc tggtgatgat aaatacagta ttattaaata
     6601 tttcaggatg tgattttatc tcatcataaa aacgaccaac ctgtgcatcg aagaatttaa
     6661 tggcagcaag gtatgcaatg ataccatttg cgcgctcaga ttcctgaaga ataaaagtct
     6721 tttctgaatc agataatccg gcagcaattt ccggaggagg tatcaatccc atcatgtaat
     6781 cgccaaactc caaaaaatcg ggatgcacgt aatcttcttc attcatggcc ttgccaacaa
     6841 gatcgtcagg taagctggta taatcgctcc acatcggatc gggctgaggt ggcattacca
     6901 ctccattgta aggcgtagca cctacaggat cattatatgg cttgtcgtaa ggaactgaat
     6961 aataatctgt gttataataa ggcaagaaat atttttcagg cacataaaaa ggaccgtgcg
     7021 ggcgcttata tcctatagcc aggaaaaatg gtttgttgca ataagtggca ggatttgatg
     7081 catagtcttc aatgaaagaa atcgctgaat ctgtaatgat ataatcaacg gtattgtctt
     7141 ccatggtgtc aggaattacc gcaaagttga atccggttac cccatcatca tgttcatcgc
     7201 cataatccaa tacatcagtc atatcgccac cttgtccgta tgagaaggct ttgttccacg
     7261 acaagccttt atcgcacatg gcaacatttg atgtatcata atcaggaaga ccaggataac
     7321 aatgcatcac tttacttaag gtatatgtga aatatcctcc gctgtccttt aataattcag
     7381 gaatggtaaa cactacttca tttccttttt cttccctgaa gttttttcgg aaatttttac
     7441 agccgatgtc atcgttgttg tacaccttag tatagtaggt gtctttaccg gtgatcatac
     7501 ttgtgcggct cggtgcgcat tttggacctg cgcaataagt actggtgaag gtggtgccct
     7561 ttttttcaat tttgttgatg tttggcgttt ttgcctgcgg gtgaccactg aatccctgaa
     7621 catagtcatt caggtcatcg gcaacgatca acacgaaatt tggttgtgtt ggcgcctgag
     7681 cccgtagtaa aacggtgccc aaaacgagag cacagagtag ggttgatagc tttttcattg
     7741 caatatgcgt gtgttagtaa gagaaaggtt atctgtaaat agttgtatgc gacaacaaat
     7801 ttacattacg gtttccattc cacccaaccg tcgccaggct ccttttatga tcatgaggtc
     7861 tgctgccggg gtgtaatctc tggcatacat gaagtttact ctttcggcaa tatgcggggc
     7921 aatggttacg gtagttccgg cagaaagcgg gtgcaatacg cccggcaaca aggtcggaag
     7981 cttttgcccg ttcgatgaaa tacccgaaga gctagatata taacccaccc acgatctcct
     8041 tccccataaa acctgaaagc aattgttaag gaatccggct ttattgtcca taaaccaaat
     8101 cagtagcgga ctgagtacta tcccgaaggt tgcaatcaat atatcaagga gtcgcttgct
     8161 tctttgtttc tgaggatgat gaatattcat ctgcacttcc atggtataga tatcacccgc
     8221 agtgtttttg gaattactgc cgataatact gtcgcttccg ggaggaatta ttttaaaatc
     8281 agtaagcttt ccacacaagg tcataagcct tacgatttcg gtagcaggca cgtcatcggc
     8341 acaaaatatt acttcattga ttctgaaatt tctgatgatg agcggtaatt cgtcaatatt
     8401 tcctagcaac agagggtgct caatgttttt attgtttcct gcaaatccaa ttaactggtt
     8461 accgagctga agtgtattta acaattgctg cgcacgtgta atttctttga cgccgccaac
     8521 aagcaatatg cgtttatcag gcgcctcgcc aaatcgtatg ttgccgtatt tgataaaatg
     8581 caaaaaacct cttgcgccga tcagcaatgc aatgtttaat gcagcgcctg ctataatcat
     8641 accgcgagaa aagcgcaaat gttcgggcag gaaaccatat acagcagcaa taatgaatgt
     8701 tccccatacc atcccgcgca cgatgcggcc aggagtggca ttgttgtcgt aaccaccact
     8761 taaaaataaa gtaaaaagcc atatcaacat gtacgccggt acattgatat ataaataagc
     8821 atcgggataa gtgccacctt caatatattt tacataatat tcccaatata atttcaccag
     8881 aaataatcca ctgaataata atgcgccatc aaaaagtggg gaagccattt ttccaagcat
     8941 acgcatcagg gctgcaattc ctgcgcggaa ataaatggcg gtattaagaa ataaaacaaa
     9001 taatcctgcc ctgggtccgc taaaatgttt ttgtgcgaaa atcttcatcg cggtataaaa
     9061 cattttaaca tagttaaagc ttgctttttt agtgctctca cctttatagt gaatgatgga
     9121 agtgtcggcg aaataaatat tcgtataccc ttttttcacg atgcgataac tgagatcgat
     9181 atcctcgccg tacatgaaaa atgtttcatc aaggtatccg atttcttcca gcacacgctt
     9241 gcgaatgagc atatacgcac ctgcaagcac gtccacttca tggttttcgt tttcagataa
     9301 ataccccaag tgatatttgc cgaaaacttt tgaatccgga aagagatttg ccagtccgaa
     9361 agccttatag aacgccactt ccggacttgg aaatgcccgt ttactttcag ccagaaatgc
     9421 gcctttccca tctatcattt taacccccat gccacccgct ttcggatgtg cgtccatata
     9481 tgcaagacat ttttcaaaag tgtcttcctg cacaacggta tcgggattga gtaataaaat
     9541 atattcgcct ttcgcaatgg ctataccttg gttgtttgcc tttgaaaaac cggtgttctc
     9601 ctgattggcg ataaggtgaa caggggtgtg agaattttga aggtcaataa atttttgatg
     9661 aataaatgat acactgccgt cagaagaatt attgtcaact atgataattt ctgccggaat
     9721 attagcacat gctttaatag cactcaacac agcttgttca agaaaatact tgacattata
     9781 atttactatg atgatgctga gtttgatcat tcgctttgct gcatataatt atcataaggc
     9841 ctgcgggtag ccaatgagcg ggcaagcgta agttcatcgg tatattctaa ttcaccacca
     9901 aaagaaatgc cacgtgctat ggaagttatg cgcacatttt gtttcgtgat ctttttggtg
     9961 atatagtata ctgtagtatc accttctatt gtaggattta atgccatgat cacttcatta
    10021 catgtgccgc tatttacttt tgcaataagg tcatcaataa ataattgctc cggccccatg
    10081 ccttcaatag gtgatatcac cccacctaaa atatgatatt caccgtgata ttgatttgtg
    10141 ctttcgatgg caatgagatc gcggatggat tcgaccacac aaatggtatg atgatcgcgt
    10201 cgcggaccgg agcatatact gcattcggca ccgggatgat cgctgaggtt atggcacttg
    10261 ggacaataat gaatatctgt tttcagtttc agcatagcgg caccaaaagc ggccacctga
    10321 tcctgaggtt gtttcatcat atgtaagacc agacgcaatg ctgttttttt gcctatgccg
    10381 ggaagcttgg caaactcatt aacagcattt tccatccatt gtgagggtag attcactata
    10441 cagtaattat aaagacgtga agttacggat cactttactt tctcgccatt gcgttctaat
    10501 aaagtgcagt tttcaagttt attgccattg gcgatgcctt tcatggtaac cttgtcgccc
    10561 ataacaacct gttctgcctc cttgaggtgg tcgccaagga agtaacactt gatgccaaat
    10621 ggatctccat ccttcccgcg gagtgcaaaa taggttccca gttcaaagtc gttgcccgtt
    10681 tcaaccacta cgccctctat ctgcaaggtc ttattgtcgt attccttgcg ataagcttct
    10741 tcatcatttc tgaactcttg tgacatcatt tctgccgata gtactgcctc cacttctgcc
    10801 ttgtcgatat ctacaatctg aaccgtctga agcagtcggg agctgcctac tatgagaatg
    10861 gccgttatga caaaactgag cagaaccatc ggaaaagtat tacttttttt gacgttttgt
    10921 gacatgaaag attcctcaga catgatcaaa taccgacctg tagcaaacac atgaaacagg
    10981 atgggaaggg cgtacctggc caacagaaag ggttcccagt tggggtatag gttgtagcgc
    11041 atgatgccat aaacagcccc gggaagcacc agccagcaaa gcagtattgt aagccatccg
    11101 gcggtattct gacctaaata gaagcgatcg gcgcccgtgc tgccgccaaa aagtgcaagt
    11161 gtaagtgtta ccttttttga tctcatgatg ttgcttgaac tgaacaaaaa tagagacaat
    11221 tgttataatg gcagggaata ctttaaaaag tgacatcagg ctgtatagtt aaaaagcggt
    11281 atttttgacc tttcaagtag gatttgctta cgcgattaag caaatttgta ggcagcattt
    11341 tctgcggcta tgttcaagtt catgaagagg gcactgtata ttgttctgtt atcatttatc
    11401 ccactaggca cacaagctca gcaataccta gagattggag cattcggtgg ggtaagtaac
    11461 taccagggag acctggcacc ggtggctttt gtacctacgg agacgcatct ttcatacggg
    11521 gctttttaca aattcaatct taattactgg ttcggactga aggggagttt gtattcaggc
    11581 tttatttcgg gcaatgacga caactccacc gaagaatgga aacaagagcg aaatctcagc
    11641 tttgtcagcc cggtacaaga ggcatctgcg gtgtttgaag tctttctgaa gcggtataac
    11701 cctaagtaca agtggaacat catgtcgcca tacatctatt ttggtatcgc gatatttcat
    11761 ttcaacccta tggcacagta tcaaggggtt tggtatgacc tgcagccctt ggggacagaa
    11821 ggacagggga atccggccta tcccgacagg aaattatatc cgcttacgca atactcatat
    11881 ccggtagggt ttggaatcaa gggttctatt tcgcccgaat ggaatgtagg ggttgagata
    11941 ggatatcgta aaaccactac cgattatctg gatgatgtta gtacaaccta cattgccaaa
    12001 gagatactgt tggctgataa tggtgatatt gcctggcagc tatcgaatcg tacggatgaa
    12061 atgaatggag gctttgaggt gcttcgagat gataaaaaat atcgtggcga tgataccaat
    12121 aaagactggt acatctttac cggcgtaact atctcgagaa actacgaagt gaagggatgg
    12181 gagtcgagaa aatacaaggc cagtgacaac ccgaaaaagc ccggtttgcg caagtccaga
    12241 aagaaaatgg attgcccggg ggtgaacctc aaactctgac actgttgctt tttttcgaca
    12301 tactaaacac tttttgtgtc atttaaacgt tatatacccg ctaagattct tttatatccg
    12361 ttctaaattg ctatttttgt taaaagattg ctacacgcaa aaaggatgaa agtattacaa
    12421 ctcctcggtt tacttacact gtccggctcc atggccgcgc agatgtccta ttttgaagta
    12481 ggtgttatgg gtggtgtagc caattattat ggcgacatga cgcatgatta tgtggttgcc
    12541 gatgaatcgc attttgccta tggaggttat atcaaatata accttgatca taaaatgggt
    12601 ttgaagttta atgtatacag cggaaccgtt tcagctgctg atgctaatag cgatcgtgca
    12661 aatcttaaag cccgtaattt gagttttact tctccggtaa ctgaagctgc atttactttt
    12721 gaatataatt ttttcggata tcgccctgta gaatttaagc agcgctattc accgtatgta
    12781 tatgccgggg ttgccgcttt ccattttaat ccgcaggcat attatgaagg tgaatggtat
    12841 gatcttcaac cacttggaac agaaggtcag gggtccgaat attttcctta ccgcgataaa
    12901 taccgcctgt atgaatttgc tattccattt ggtgttggct ttaaatgggc actcaccgaa
    12961 agatggaata tgggtttgga atatggcgct cgttggacct ttacagatta tttagatgat
    13021 gtgagtcgaa cttacgtaga ccgcaacctc ctgatagaat ctaatggtat cgatgcttat
    13081 aacctcagca atagaagcgg agaatatctt ggtggcgaac catttgacta tggcaataac
    13141 gactggcgcg gcgatcctac caataccgat tggtatatgt ggttgggaat caccttcagc
    13201 cgtaacctga ttaaaggtct tgatgaaggt ttccgcactt cggcaaaaga ccagcttggt
    13261 tgcccgggac cacgtcatat caacaaagtg aaaaagaaga agacataaat ttcctgaatg
    13321 gaaataacga aatagggatg agcatacact catccttttt ttgtgcatat cctaaaaagg
    13381 tgattcaata aaacctgcta ttttgcaggc gataaggaca atacattatg gcaggttggt
    13441 tatttctttg tgtgctttta gcctatttcg gattgttgtt tactatatcc tggtttaccg
    13501 gcagaaaagc cacagatagc gcctattacc ttggtaataa atcttcgcca tggtatgcag
    13561 ttgcttttgg tcttattggt gactcattga gtggggttac tttcgttagt gttcccggaa
    13621 gcgtagcaac acaacaattc ggttatatgc aaatggtgtt gggttatctg gtgggatatt
    13681 atgtgattgc aaaagtattg ttgcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnatta aaactttggt gtggacagat accttacaaa gttcattttt actggcaggt
    13861 gtgttgttat caattgtagt aattgcggga gcattgcatt tagatgcagg aggaattgtg
    13921 caaactgttc gcgacagtaa ttataataat atatttttct gggatccgat gaaacagaat
    13981 tatttcttca aacagtttat cagcggcgca ttgattgcca ttgtaatgac cggtcttgat
    14041 cagaatatga tgcagaaaaa tctcagtatg cgcaccttac ctgaggctca aaaaaatatc
    14101 atgtggttta gctttgtgct gatatttatt aatctgtttt ttgtaagcct tggttcattg
    14161 ttgtatgcat atgccgacag tatccaattg acattaccgg caagaacaga tcatttattt
    14221 cccatgcttg ccttagatca tctgggcgta tttgcggcaa ttgtatttat tctggggata
    14281 tcggcagcta ctttttcgag cgctgattct gtgctcacta ccttaactac cagtttttgt
    14341 atagatgtgc tgaaatggga tccaaatggc aatgaaagtg gtaagcatag acgtcaacgc
    14401 catttaatac atgtgttatt tgccgtgcta ttgctgatag tgataattgt atttaatgca
    14461 ctaaataatg atgctgtgat aaatcaggtg tttgtagcag cgggatatac ctatgggcct
    14521 ttgcttggat tatttgcatt cggcatttta acaaaacgca gtctcaatgc gaaggcggta
    14581 ttactgatat gcttgttgcc gcctgttgcc tgttattacc tcaatgagaa ctctgtaacg
    14641 tggtttaatg gatatcagat aggtaatgag ttgttgctca tcaatggtat tttgaccttt
    14701 gtgggtttat atgtaatttc gaaggctcct cagcaaaata atattcaaac ggcttaatac
    14761 atttttatgg ctgaaaagta tcgcaagaaa aaattgttta aaacaaaaga ctggactgaa
    14821 atcagtgcgc agtcgagctg gcgcatgttc aaaatcatgg ccgaatttgt agatggattt
    14881 gagacgatgg ataaaattgg tccctgtgtt tcaatttatg gctctgcaag aacaaagcct
    14941 gatcataaat tttataaact ctcggagcaa attgcagcgc gattggtagg agagggtttt
    15001 ggtattataa ccggcggtgg acccggtatc atggaagcgg gcaacaaagg cgcttataag
    15061 gaaggcggta aatctgtggg attaaatatc gagttgcctt atgaacagag tcacaacccg
    15121 tatatcgatc gcgataaact gatcaatttc aaatacttct ttgtgcgcaa ggtaatgctt
    15181 gtgaaatatg ctcaggcatt tattttcatg cccggtggat ttggtacgct ggatgaattg
    15241 tttgaagcat taacgcttat tcaaacacat aaaattgaac gtgtccctgt tatcatggta
    15301 ggtaaaactt attggaaggg attgctggca tggatgcgcg aaacaatgtt gcacaaagaa
    15361 cataatatca gtgaagagga ccttgatctg ttttttgttt ctgatgaacc cgaagaaata
    15421 gtgcaacata tcaacgcatt ttatgccaca tcacaccttc gcccgaattt ctgatgaagg
    15481 aaataataac cttacagccg ggagataagg tccctgcatt tagtgcctcc gatcaatacg
    15541 gcaataaaat caccgcagcc tccatgaagg ggaaatggta tgtgttgtat ttctatccta
    15601 acgatgacac ggaaacctgc acaaaggaag cctgcaatct gcgcgataat catgctgctt
    15661 tgctaaaagc cggaatcacg gtgataggtg tatcgcatgc accggtagaa agcaaattga
    15721 agttcaccaa aaagtataac ctgcccttta ttttacttgc agacacggat ctgaaaatat
    15781 gtaaatcgtt tggagtttat ggcgataaat tatttatggg gaaggtgatt accactattc
    15841 atagaattac ttttctgatc aatgataaag gtcgcattga gcacgtgata tcaaatgtta
    15901 aatccggaca ggcggcgagt cagattctcg atctgatgaa aaaatgacac tcaataaaat
    15961 ttgtttttct aatttcgtgt ataaacagat aattcaggca tatgttttct gcgcgacagt
    16021 ttccaactaa gttgatcagc gctgctttcg gcgtgttgat cattatgggt gtgataagta
    16081 tttcattcgc aaaaaatgat ggggcacctt ccggcaacac cggttctccg ggtgatgatc
    16141 agacttgtgc ccatacgatg tgtcattcag gaactgcctc agaacaaata ggattgataa
    16201 gcaccaatgt gcctgcgagc ggctatctgg caggagaaac ttatttggtt accgtaactg
    16261 ttaccgaagc cgatattgtg aaatttggat ttcaggcttc accgcaaaac cttgatggag
    16321 acaggatggg tgacatgagt gtaattgatg ctacggagac taaacttacc ggtggaggaa
    16381 aatacatcac acataaatct acaggaactg caggctcagg atccagaacc tggactttca
    16441 actggacacc cgatgaaagt accggagaag ttggatttta tgtggcggta aatgcttcaa
    16501 ataatgaaga cgatgcttcc ggagatcata tttattatga tgcgattttt attccggaag
    16561 acccggataa tgtgccggtt aaagtggatg atattgctac tatagattgc actgttaatg
    16621 ccatctcgaa tgatatgatt tatgttcaat atcaggcaag tggtgttcat gccgttaagg
    16681 cacagatatt tgacatgatg ggaagagaat tgttggatga agaaaatgat acacaagcat
    16741 caagctttac attagatact tccggtttgt cggccggatg ctatctggta gtattgacaa
    16801 gtggaactgc cagatttgtg caacagatcg tgttaccata aaagaaaacc ctgccggttg
    16861 agcagggttt caaggtttct gttaagttat tatttattcc gatttttcgt cggtagcttt
    16921 ttctgcctct gcttctttag ctttttcttc taattcggtt tttaatacac ctttttcatc
    16981 gagtatggca aaccatttga tgaatttttt aatatcgctg atatgtactt tttcgcgatc
    17041 atattccggt aatacctttt cgaagtaagc actcaatgtg ccattatcgg cttttgcatc
    17101 gacaggtgga ttgctatctt tttgctcata cgctttgagc atcacttcca gtaattcaac
    17161 ggtatcatca ctcgtgtaaa tggcaatatt ttcaagtggg gagaagaggt gttcgcgatt
    17221 ggaaataaat tttgtccatc cttcggcaag tgaagtaaca atgagtcctg atgctttttg
    17281 tgactccatt ttatacaaac cgccaagtcc tgttattgcg gtaatctctc tgaaacgcat
    17341 atcgtaattt tgtgcaaaat taatgaaacg ttttttgtta taaggtgttt attgcaataa
    17401 aggtgccatt cattttccgt atttttcggg tttttccata tgccatttga actgatcagt
    17461 caatattccc ctacgggcga ccaacccggt gccatcaaac aacttaccga aggattgttg
    17521 gccggtgaac catcgcaggt gttgctgggt gttaccggta gtggtaagac ctttaccatt
    17581 gccaatgtga ttcagaatct gcaaaagcct acgctgattt tatcgcataa caaaacattg
    17641 gtagcacagt tgtatggtga attcaagcag ttctttccga ataatgccgt ggagtatttt
    17701 gtcagttatt acgactacta ccagccggag gcatatattg ccactaccga tacttatata
    17761 gaaaaagatc aatcgataaa tgcggaagta gaaaagttgc gattgaaaac tactaccagt
    17821 ttgcagagtg gaagacgcga tgtgattgtt gttgctaccg ttagctgtat ttatggcatg
    17881 ggtaatcctg ccgaatttaa ggacagcatt gtgcgtgcaa atgttggcga taaaatgtcg
    17941 cgcaattatt ttttgcatcg cctggtagac gcattatata gtcgcgcggc ggtagacttc
    18001 aagcgaggaa attttcgcgt gaatggcgat gtggtggatg tgtttccagc ttatgccgat
    18061 attggttatc gcatcacttt ttttggagat gaggttgaag agattgaaac attcaatcct
    18121 gagaatggaa aaaaactgga acgcattcaa aatatcgcca tattcccggc taacctgtat
    18181 atcgctccaa aggatatttt gccttccatt atcactgata tttatgcaga gctggatgct
    18241 caagtgaaat attttgaaag catcggaaaa tatatcgaag caaaacgctt gagtgagcgc
    18301 gtgaattttg atatcgaaat gatcaaggaa ctcggatatt gcaacggtat cgaaaattat
    18361 tcacgatttt ttgacaggag aaccaaaggt cagcgaccat tctgcctgat cgattatttt
    18421 ccggatgatt acttgcttgt aattgatgaa agtcacgcta cagtcccgca aataggcgca
    18481 atgtcgggtg gcgaccgcgc acgtaaaatg aacctggtgg aatatggatt cagattgcca
    18541 agtgctttgg ataacagacc gcagagtttt gatgaattca tgaccttatt gaatcaggtg
    18601 atctacgtta gcgcaacacc cggcgatttt gaattaaatc aaacaggtgg ggtagtagtt
    18661 gatcaggtag tgcgaccaac aggtttattg gatcctccaa tcgaaatcag acctacatta
    18721 aatcaggttg atgatttgtt gcatgaaata gatgagcgta taaaaaagga tgaacgcata
    18781 ttagtaacca cgcttaccaa gcgcatggcc gaagagcttt cgaaatatat gctcaagatg
    18841 ggggtgcgtt gtaaatacat acacagcgaa gtagaaacac ttgagcgcgt tgaaattctg
    18901 cgcgatttga gaattgggag aattgatgta gtggtaggtg tgaatttatt gcgcgaaggt
    18961 ttagaccttc ctgaagtatc actggttgcc atcatggatg ccgataaaga agggtttttg
    19021 cgtaatacac gaagccttac acaaactgcc ggtcgcgcag caagaaatgc aaatggtatg
    19081 gtgattatgt atgccgataa aattacagac agcatgcagc gcacgatcaa tgaaactact
    19141 cgtcgcaggc agcgccaaat ggaatataat gctgaacatg gcatcacacc taaaacggta
    19201 attaaatcta cggatgagat atttaccatt ggtaaggata ataagaagtc ggtacaagca
    19261 tatattgaac cacaggatgt atcactgagt cttgttgcag atccggtggt gcatatgatg
    19321 ggcaagccgc aattggaaaa agctattgcc gataccaaga agaaaatgct tgcagcagcc
    19381 aaggatatgg atttccttga agcggcacgt ctgcgcgatg aaatgtacgc actcgaaaaa
    19441 ttgctcgacg aaaaatcctg atcaggactt attgatcatt acactacagc gcacttttcc
    19501 attttgcatg gcggtgagca tgtaatttcc tgatgcaata tttcccaatg ggaattgaaa
    19561 tgtctgtgca ccttgaatgg tgctattttc catgaacgta tgcacggttt ttccatcgat
    19621 ggaggtcaat aataacgacc aggttcctga tgtatttccg gtgatggcaa agaatgctgt
    19681 attgtcgtgt gcatacgctt gtgcaaatgc ggtttcaggc gtatgcactg tgctaaatcc
    19741 acatgccaat atctcagcag ctgcgcgtgc accgctttcc agcgcgccat gtactgtagc
    19801 tgggtgatta ttattcgttg cctcgcctgc aaaaaacaat ttacaatcaa caggtgtggc
    19861 aaggtcgaga cgtgtggtat ccgtttctgt tgtgtacgtg ttgggagcag ggaaggaata
    19921 agcgccttta atatgaggct ctttgctcca gtcctgaata aatccatcca cataattagc
    19981 agtggcggcg ccatcgaata aggcatcgag ttctgcaagc gcaacatcaa tagcacctgc
    20041 atctaaggca ctcatgtatt ctgcgttttc gcccatgatg aagcaaatga gcacattatt
    20101 ggtagcaccg gttttatcga tgcccggggc ccacaaaaaa gtagaatatc catccaccgt
    20161 aatatcacca atatcgccac cccaaaaatt cgtactgaat tttaaaaata ttttcatgcc
    20221 tgctcccata tccaatgtat cgatggcagt ttgattttgc atcggcagtg gaggattaaa
    20281 tattatttcg gcattcttaa gaatggggag tggtactgcg atgatacatt tatctgcaga
    20341 aaatgtagcg ccacctgcaa cagttactgt aacaccgggg aaaaagtaat caattccggt
    20401 aaccacctga ttgtattgca ctttatcaag tatgggtgcg aagaacaagg tatcaagaag
    20461 atcacgataa gaggtgtcaa gcaaataatc tttgccgcct gtcagccata ataattcact
    20521 gatggcaatg cttttcatac cgatgcgatc tacgctggtt ccaaattctg agcctatcca
    20581 tgcttcataa aaatgccaat aaggatgcgc aggtgtaatg ccatattcag tatcaatata
    20641 ttcgctcatc agcatttcat caccggtata ggcatacatg ttcagataaa actgccaaca
    20701 tgtttccaga tcggcatccc aatatggttc agttttaatg acaccaccga tatcatacat
    20761 ctctttatgc gagccatatt ctaatagcag atcagggtta taatcattga tagaactcca
    20821 taaaaaggat gggggagtgc ctgtcacgtt tccttttcca tgtacaaatt cggcacctgc
    20881 ttcggtaggg aaatcggcaa aacccggtaa gggacgcacc ctgccgccat gggtgtctga
    20941 tgcttccaat atggtaacat caacaccggc atcctgcaat gcgcgcgcgg catataatcc
    21001 ggcggctcct gccccgatga tgagcacact gcccactccg gcttctttag gcaaccactt
    21061 gtgccattcc ataaatgagg gtccgaatac tactgccgga ctggccattc caagattcct
    21121 gataaagttt cttctgcgca tgagacgaag ttaatgtggg aatgtgagaa tgtgagaatg
    21181 tgggaatgcc tgcctcgccg caggcgggtg agaatcccgc tatggcggga tgtcgctgcg
    21241 ctccatgtgg gaatattttt tttgcgatga cactagattt cccccggcgc ctctgcctct
    21301 gcgcctttgc gtctctgcgc gaaacacatg aggatctcaa tgtgaagtat aaccataatt
    21361 ctgaataaac agtataagaa gaataccaac cgctaaacac atctcgaaac ccggtgcgca
    21421 tacggaaatg aaaatcatca gttcctttca aatccatgtc cggataatga tcattatctt
    21481 agaacaaaat gatatccggt tcaatatcta cctatacaat ctcggcgtct agtgatccct
    21541 tcaacatcaa aaatgatgcg gccataaggg agtaggattt aacgtctagc ttattcatgg
    21601 gtggggggat ttatgcaggc taaattaatg aaaatgtacg agtaggagca gcgtattttg
    21661 tagtgtggtt ttgtggtacg gatgtaagat caaaagcgcg gagttcgcgg agggcgcgga
    21721 gggtacggag gtgcggtttg gattgtcggc tgataatgaa attttattat gactaagtgg
    21781 tttatttttt ttaaaaaagt ggaaatgaga ctttgttgtt aggtgtaatt tatgcttcca
    21841 ctatcacaaa ctccattgtt ttcgcgatgt gatcttcacc tacaatgcct tgcgcatatt
    21901 tgctgttggc gccggtgtta tactttgtca ggtaggccag atttatcttt tctgtcatac
    21961 ttgctatgtc tgccgggacg actgccttaa tggcaatgat attgttgcca catttaattt
    22021 gcccatgcgg attttgcaaa aaggtattat accaactcct ttcagcgaaa ccccaggaac
    22081 gggcaaatat tcgatcttcc accacaacca tccaaatgtc caaaaacccg ggtcggtcaa
    22141 cccctgcttt tattccggtc aggctattgt tttgtatatg gtctaaggct atttttttgt
    22201 gcatattcag ctagaggtaa ctgaaaattg gtgggaaggg tgcggtttgt ggggaaaaaa
    22261 aggattttta tgtagatgtg ggttttgagg cgatttggtg tagataatga tcttgtttta
    22321 cggctaaaac cgcgcgggtt taaaatgaac ctatgcgtct acctataatg taaaataaaa
    22381 agtccaaaag taatttgaga cttggcagca taactttctt ccttgtaaac agaaagacgt
    22441 cagcttgtta gccatcttga tttaggctac tattcaggta ggtagtcttt ttttcttata
    22501 cctttcgttt tgattttttt aagaattccg ttttcattgt acgtttctac gtatacaata
    22561 ggttgattca gtaattgttt ccaatatccg taattgttgt ttttacaagc aatgcgggga
    22621 caacaataaa atcctaagtc aaatgaaaag tatacaagct tttcatctgc ttcaaaatta
    22681 cttgtagtgt ccaagttgta ttttgagaga agctctgcaa ctgttttccc aagagcaaag
    22741 ttgtaatatt cttgaatttt attcaaagtg tcttgtctgg tttggcagtg aactgcaaaa
    22801 tgcaaaaata gtagcaggag agtgaaagta aacttagata aaatgttcat tgtgtctcac
    22861 tttaaataac ctctaacggt ccgtggcttt gcgcagcgcg ggagtttcgc ccaaacaaat
    22921 gtatcaattg aatcttgaat ttacgagcaa ccttgctctt attggattcc tcgaatgccc
    22981 gcgttaagaa aaactgcggt tacatgttgg atttattgtt tatttatttc tttgttttct
    23041 ctaatacaat ccaaataata ctttctgttg tatgttccgg gagatgcatg atctccaggt
    23101 tcaaaatcaa aatgcacatt gttgatattt ggcatcccgg tcaacgtaaa tgtagcggct
    23161 atcatatatt catcagcacc agtcgtcccc atttgttgtg ttaaatattc actattatca
    23221 atcttaacat agataatgtc cttagaaatt ttcaggtatt caagcttgac cttatcccga
    23281 tattttgcgt ttatggtgtt aatcaaattc cactatgtca aagtatctat ttttattttt
    23341 ctcagttgaa taatcgtatc ggccatataa tcatatctcc agatagaaat attgtctccc
    23401 ttgtttcctt ggtttgtttg aacaaggctt aatgagtcag gttgattttt caaatccttt
    23461 gacacgccgg catgttcatc cgatttaagc atgtctgtct ctgaatttac atgtctaccg
    23521 caagcaaata gaagaatttg gtaacgtttt ggcgcttggc gaagaagcgg atttcgaagc
    23581 gcaaatctgt cttcctgaag tggaaccgac atattgcatt gcatttcgtc aaaaacacag
    23641 atcaagcttt ttcgctaagc gcctgttata accagtggtt taggatcaat tcctagtcaa
    23701 ttacaatttt cctgcaagtc ctttgtatgt ctgcaattaa ttcaactaaa taaattccac
    23761 tcggttgtgt catttcaacg gtcaggaaac cactattcat ggttctattg taaacaatct
    23821 cgccagtaat gttatagatg tttaaggata tattttctaa aaattctgga acactgatat
    23881 aaacaaccga tccaaatgaa taaatacttg cattaacaat ctcattgtct gcatctggaa
    23941 gaccggttcc gaaatgtggt aatgtatcgt gggcaaattc tacaatccta ccaacagtat
    24001 cgccagcgat tattggcaca tccggcaata attcataggc gtaatctttt atcacaagtt
    24061 catttccccc gtccagaaca ctacagcgaa tccaaccgta gtgggtttta aattcgccgc
    24121 cttcaaaata tttcaattta actcctatga atccatctag aaattctgga taccagtatc
    24181 ctccactcca ttcaatgaac ggagaggaca attgggaaaa gtgatacatg agttctccac
    24241 ctgcgaacag gaaactgtca tttatttcct cacccgcaat aaatggcagc aataaggctt
    24301 gagagcccaa aaaacttccg gcataaaaat atgaaaattt gcctaaatat ccataaggcc
    24361 aattgccccc accatgaata tcatgggctt gaaaatgcat cgaatatgtt gtagtcccat
    24421 cccatgacga aagcatgaaa aaaacatccc agactccgtc ttcattaaaa tcaatttcca
    24481 ttccttcatg aattgttaag attgagtctg gatcaatatc agtataaacc gctttagctt
    24541 ctacctttgc attaattgtt aaaaatgaag tggccattgc agcatacgca ggaaacgtga
    24601 atttattctt cataaattac gattttataa atgatagctg ccgccatttt gcaaagcttt
    24661 tgttatagga tgtggtcttt cgattattca attgctactt tcctagtcaa aatattttca
    24721 ccttctttca aagtaactaa ataaattcct ttggggaagt aattcatgtc tacaatttca
    24781 aattgattgt atattgactt atctaaaata gtctgcccgg taagattaac aattgttaat
    24841 tgtgttggtg tgtaattgtg taaattaata taaatggtag tattataatt ataaaccgtt
    24901 gcaaaaagcg aaggctgttc ttggatattt acaaagctta ctgtatcacc ggcaataata
    24961 ggttgatccg gttgcaactc atatgcataa tcatgaataa ttaaagttcg tccgctatct
    25021 accaccgaac aacgaatcca tccatagcgc atgatagcct cctcgtctgc aaaccgtata
    25081 gcaacatatt catttaaatg ttcataacca gaataaaaag aagtccaatt tccttgtgga
    25141 agaacaaaag gaacatctat tttgtacttg agtgctacta atgattgata attactgtta
    25201 aagaagttca aattttcatc aataataact ccattttgca tggcgtacgg ccaatagtag
    25261 gtatacccac caccgccaga taaaaatcca gtgctaccag ctatcccatt taagattgta
    25321 tcaaatgcac cgacaaaaag tgctttcaca tttgccaagt ccccataaaa aaccgtagtt
    25381 ataaaacttt cattaaaaaa attaaaatca ttcaacccgt catcatccaa gtcaatgcca
    25441 aacatttcat tcggttcatc tagcaccaca tcggggtcaa tatcggtata aaccacctgt
    25501 gatgaagcag cagtaacccc aagaagggct gctgctgctg ttgaatattg taataaagaa
    25561 aatttgtttg ccattattac aattttataa aagattttgt aacattactt ccatcactaa
    25621 aggtacaaat tacctgatac atgccataac tgagcattga aacatcaatt atattacaat
    25681 tatcaatagt ttcaaacatt ttggctccgg tcatcgaaat aactgctact gaaacaattt
    25741 gttcggtgct tgaaatattt atgaggttgt ttgccggatt agggtaaatt gaaagcgaag
    25801 tgtttatttc atccgccatt cttggaacat atggcactac tgccattttt acatacccgg
    25861 acgtaaatcc cggaacagta atacacagac taccatctgc ggcagtgcaa accggattaa
    25921 cgtaattata tgtttgatca atttccatgt catacggatt ggcaggcgta tagcacgaat
    25981 tgaaatcata aatcatgctt ttcccacttt gcgaatatgc ctgaaaagca tctacaacaa
    26041 acatttcagc atctaccagc tcaacctgat aaccatacat taaatacatc cctgcaggat
    26101 ttaatacata ccgctggtca ttgcaccgac tattagtaaa atacatatag acaaaatctt
    26161 tggcaggggt aataaaaaat gttggcggca aattgggatt gtgcccataa gtagctacag
    26221 ttgccggaaa gtaattaaga ttattcttgt ttatttcgct gattaaatcc atcgtatacc
    26281 ctacggttct ttttacatag taattccttt tggccggatc acctgctgct aagttgtatg
    26341 gtgaaacatc ctgtccaagc agtgtaagtt caacatcttc gtcagcaggg gtgagcattt
    26401 gaacaccaga gcctccggca tagttttgta aagtagcgta tttgaagaaa ttttctcgat
    26461 aaccttcagt aaaatttagt tttaagtttt ttaaccacca ctcctgtaac agtactgtgt
    26521 gctgataact gtttgtaaat atttttccgt tatttggcgt gccttcatct tttaaattcc
    26581 attcagtaat ccacatgtct tttttgattg aagaagtgag attgaagttc aagtcaaggt
    26641 taaacaaatc aaggtcatta tcgaatcggg ttaacataaa ttttctgaaa tttaatcttg
    26701 ccgcatcaaa agccggttgt atgcgggtat ctgcattatc aaaatcccat tcagggcaag
    26761 aatagcttcc taaaatacct gtttcggtta cacattcatt ataccatgaa tcggcagaaa
    26821 aataggtgtg caaaataact gcatggaatt tttttacact ggttccggca tgagtgtcac
    26881 cataatggga gcgcaaggca acattccaat caactgaccg ggaacccgca tcctcagctg
    26941 gtaatatatt aaaaacttca cccgttccat taagtccttc ggcgcataaa ccaattttat
    27001 agttcacacc agacctgttt ctaaagacat agaaaaaatt tctattcgca gcgggaataa
    27061 ataaagattc accaattgct gcttctttac ttgcttgacc cactacagcc tgaccatcca
    27121 aatattgcca ataattttca aattcatcaa actgcataat ttgtttgtga aatttatttg
    27181 ccgtttcgtt acccatttca acgccaacta cattaacatt gtgaataggg tttgattgaa
    27241 ggtattgaac aatatccaga caatcttctg ccgtttctgt cagaatgttc aggcatacga
    27301 ttacatctac tttgtaggtt gggtcaaata cctgattatc tgtttcaatt ttttgaatta
    27361 aacgaataaa atggtcgata aatcggtcgc cggaagttaa atcttcttga tatccataat
    27421 cagttcgata ttcttcaaat gcatctaata tttctgaact tccgccaaac cagtttaaaa
    27481 gtgttgttac atttgtttca ttcataatat ctgttgcatt tgttggtgct tctaaatcat
    27541 tatcaattgc atctaaatat cgagtaattt caacgatgtc ataaccatat cctttgtcgc
    27601 cggtaagcaa atgcatgaac ttactgcttg aaccacccgg aaagcgcaat acttttggac
    27661 gcatgtcgct aagccactgc cattggtctt cactgtaaat atctccgtat aacgttgatt
    27721 ttgtaaacat gcccgttacg ttaactccga attgaccttt atttatggtt tttacagttt
    27781 tatttaagtt aatactcatg gttgaagcag gcgttgcgca atcgagcgat gcacctgttc
    27841 taaatccggt ggtttctgag gtagaatctt caattacata aatggtatcc attccactat
    27901 tgtaaactgt gtcggcaagg tcaacaattt cttcccattc aagggtatca atatcttggc
    27961 aataaatttt attgttacaa aatacaaatg aaataatata tattgataaa accaacatgc
    28021 gctgttttat gattaaattt ttcatgttaa taaatttaag gtaaataaaa acaagtgaaa
    28081 gtgtgttcct aagggattat tcataacact acatcgtcgt ctacgcttgt tcagtctgtc
    28141 aaccgaccag attgtgaatt gctcactccg ctttttggaa ctgcgaactt gatagaccat
    28201 atcctataac gttccgaagg taggcgcatt ggcccaaata aaacgttata atattctat
//
LOCUS       DAOQOU010000023        27981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_773_length_27981_cov_5.277376, whole genome shotgun sequence.
ACCESSION   DAOQOU010000023 DAOQOU010000000
VERSION     DAOQOU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 27981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27981
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_27981_cov_5.277376"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1939)
                     /locus_tag="PLM90_03560"
     CDS             complement(176..1939)
                     /locus_tag="PLM90_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88476.1"
                     /translation="MQTWVEPDTTLDNFAMFNPARQDLFENAWLGNLGSPYTPRWFDY
                     HRELGFDYGRHERDAYITTLDEVKYYRTNVPFTRLYYLAGQSEEQLFHVTHTRNFGPQ
                     ANVAIDFSKLVSTGYYQYSRNDYGNTTLSGWYTTKNNRYLIEGAAIRGKNNNEENGGI
                     NNDTLFNYPNPELAEPFRTQAFTEWSNWQGRITQSYQLGKNFSYQINDSTTGNYFDPK
                     FVITHTLTGIDHYYLFEDPATDRTYYGTLLYESDTLRDRTSVRGFENKLQFATSLTHR
                     VSVDSVIPAKLFAEIYALQQHHILSDQAGKQDYDHFIIGTHLLSPSVGKHGGRLGIDA
                     AYDLINKNYDTKLIIQFDSINPESHIFVRYGLLNPTVIESHYSGFRDEWNGSGMPTVF
                     SLGLNAESKPQHFTFHASFSMFKDYNYASTSTWFEEHYLHTNTLDFNIGQAYIRKKIT
                     LGNFVFDNVAGAQFAMDITSIPNFIYTGDWYVQGNLFKNALYSQAGIHFWGSSAYTPM
                     KYDMITGRFLEFASYGTNNSSTFPPVIDLFASFDIRTFRFFVRMDNIAQGLFNKGYYE
                     APNYPMQPRSFKLGINWDLFY"
     gene            complement(2127..2951)
                     /locus_tag="PLM90_03565"
     CDS             complement(2127..2951)
                     /locus_tag="PLM90_03565"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMZ88477.1"
                     /translation="MDSLLDRLNEAVHFIRQRTDFQPEFGIILGTGLGNLVSHIDIVA
                     EINYHLIPHFQVSTVESHSGKLIFGSIGDKKVVAMQGRFHYYEGYTMEEVVFPVRTMK
                     LLGIKRLFISNAAGSLNSNIEKGDLMILRDHINLQPSNPLIGPNEYDLGPRFPDLFRC
                     YDENLIETAMSIARDNNIRCHKGVYVSVPGPNLETPAEYNYLHIIGGDAVGMSTVPEV
                     LAAVHMGLPVFAISVITDKGYPMEEIEPVTVDDVIAVAKEAEPKMTLILQKLIAQL"
     gene            complement(2984..4033)
                     /gene="lpxK"
                     /locus_tag="PLM90_03570"
     CDS             complement(2984..4033)
                     /gene="lpxK"
                     /locus_tag="PLM90_03570"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HMZ88478.1"
                     /translation="MRWLLLPFMCLYGLVTGLRNFFFTKGWLRSFEFDFPVILVGNLS
                     AGGTGKTPHIEYLIRLLKSSYKVATLSRGYKRTYTGFGIATELSRVEDIGDEPKQYKQ
                     KFPDIEVAVSIDRVGGIYQVLHDEPQTQVILLDDGFQHRRLRAGLNIVLTTFQKPFYD
                     DYLLPVGRLRETRAGKNRAQVIIVTKCPFDITVEQQQEIMKKIGPQNDRQVFFTTLKY
                     EALYPLFADQDISLEITKPVMVTGIAGNKDMFAHVSTMYADITKMSFADHHYYSVSDI
                     KRIKERGDTIITTEKDAMRLMEQKEAILANGLKVFVLPVEVVFINKADVFEKLVLDFI
                     VQSTPSPTETTVSNE"
     gene            4359..4748
                     /locus_tag="PLM90_03575"
     CDS             4359..4748
                     /locus_tag="PLM90_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88479.1"
                     /translation="MNRIKNYPPDPMHFAISAGLKIMVVPCFYWLAVFSATLFEKPVL
                     VKWLLLDDSVIMQALLYPVMIMLPLITLLINIKARYERRIVDAVRSPFANRVQFGVMG
                     MALLSLIGVFTYTFFERLDFWMYITGK"
     gene            4745..5266
                     /locus_tag="PLM90_03580"
     CDS             4745..5266
                     /locus_tag="PLM90_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88480.1"
                     /translation="MKNYPPFGFVFRIWLYTNLLGSLLTGLFFALSESISNDFFSSIL
                     GTFFLFIALALFVGLWRSLPALLLTLGFVTFIVPYFKKTKPAFIATIAFIELLCPLTF
                     YIYFFRNRPEDLFENLLFTGIIGAYMLCILLMTIKHRKHFFTPLSLSDDPVNSEQSTS
                     EEILINSNQSDHV"
     gene            5259..5474
                     /locus_tag="PLM90_03585"
     CDS             5259..5474
                     /locus_tag="PLM90_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88481.1"
                     /translation="MFRQYGWFFIPASIVGVGIAGAALIFIVQTFLVIDSQSHSASDT
                     LYGWYPYAVTTFLAYQWIAGNLKDRKS"
     gene            5554..7698
                     /locus_tag="PLM90_03590"
     CDS             5554..7698
                     /locus_tag="PLM90_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ88482.1"
                     /translation="MQGWSLLMDLSTPFKEPVIIFSIVLAIILLSPILLKTLRIPSIV
                     VMIIAGVIIGPHGFQLLERDGAIVLFGTVGLLYIMFLAGLEIDLHGFRKNISRSVSFG
                     LFTFIIPFIIGFAVCRMILHLDMIPALITASMFSTQTLVAYPIVSRLGITRSAPVAIT
                     VGGTIITDTLVLLLFSVVTNFAMDKSDSFFWLRLAISVIAFAAIVLYIYPIFAKWFFS
                     KLENEGGSQFIFVLTLVFIAAFLSQLAGLEHIIGAFLAGISLTRLIPHSSTLKNRIVF
                     VGNNIFIPFFLINVGMIIDLHVLIENGGALTVAAILVTVALSTKYIAAWITQKTFGLT
                     RNEGSLIFGLSTAHAAATLAIILVGFDLGLLDEKILNGTIIVILVSCLVSSFVTENAG
                     RKYVLLTANKVDTDPEVMERILVPVGNPDSAEKLLDLAMMIRNPGSKEPIYPLAVVED
                     GPQAREQLIRARRNLQLAAEHVSSSETKVQVLTRVDVSIAGGISGAVKELGITEVVLG
                     WHSRVSTSDRLFGSIIQQVIKSTDQMLWIGRLSQPLNTIDRIAILMPPNAEFESGFFR
                     WLEGVKQLSQQTGARCMFWGERKTLEIVDAVNNAKFRLIAEYSEFTDWDDLESLQSIV
                     KTGDLLVVVSARQKSVSHFSALDQVPGRMHTIFGDKSFILLYPYQSEFRSGDMNFEYS
                     VLTPSAFKENIARINQIGKIVYKAFTPGKEKK"
     assembly_gap    7726..7825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8232..8331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8491..8566
                     /locus_tag="PLM90_03595"
     tRNA            8491..8566
                     /locus_tag="PLM90_03595"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8526..8528,aa:His,seq:gtg)
     gene            complement(8782..9552)
                     /locus_tag="PLM90_03600"
     CDS             complement(8782..9552)
                     /locus_tag="PLM90_03600"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMZ88483.1"
                     /translation="MENSSNNSRRSFLIKGTLAALSVSFLNSFRFPAAHAASAPSLAE
                     FNPEGGTFELPKLDYGYDALEPYIDARTMEIHYSKHHQAYVTKLNEALEKAPELKGRA
                     LPDLIRNINELPEAVRNAIRNHGGGHFNHSMFWKLMSPTAKDSKASAELEAAINAKWT
                     SMDNFKTEFSKSASTVFGSGWAWLIVDKENNLAITTTPNQDNPIMDVAALRGRPILGI
                     DVWEHAYYLKHQNKRVDYIADFLNVIDWNQVSKWYGER"
     gene            9658..11763
                     /locus_tag="PLM90_03605"
     CDS             9658..11763
                     /locus_tag="PLM90_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMZ88484.1"
                     /translation="MQKSGENISLQVEGMTCSNCALGISRLIEQKGAHDIYVNFATGD
                     VRFTLTPEQKLSVFVDGINGLGYKVISGNAEHSERKKKLSDIEIKFLITLPFTFLLLL
                     SMVPGLSALHQPLVQFLLCLPVFAIGFYHFGKSALGSIRSGIMNMDVLIFTGSTAAFV
                     YSLYGWYSGNFMSMMFFETSASIITLVLLGNMMEHRSVQQTTTAIRALQKLQPETAKR
                     VHFDLMAGGDHIDEIPADQLQKNDVILVNTGDRIPTDGIVMHGSAWVDESMLTGESAP
                     VYKQQGDVVTGGTISVDGSIKAKVTALPANSVLASIIEMVRRAQVDKPKLQRLADRIS
                     AIFVPVVLSIALLTLLISHFAFHMSFENSLMHSIAVLVIACPCAMGLATPTAVMVGLG
                     RSAKNGILIKGAATAETFAQTQVIIFDKTGTLTEGDLKVSGMQLFDCDAEFLGSLIQG
                     LEVHSSHPIAQSLLRHFAGSSKMNFDNVTEIKGKGISGKDAIGIEYQIIAATADQQPF
                     DIVVLRNDKLIAGLQLEDSIKQDAAEAIAYFNERGIETILLSGDNAKKCAQVAEATGI
                     STVYAAKSPAEKLELVKQISSTRTVTMVGDGINDSPALESAHVGISMSNATQIAIQSA
                     QIILLNGHLQKLVDAHKLSRATLKTIRQNLFWAFFYNILAIPVAAIGLLSPIIAALSM
                     AFSDVFVIGNAILLRSKKI"
     gene            11769..13055
                     /locus_tag="PLM90_03610"
     CDS             11769..13055
                     /locus_tag="PLM90_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMZ88485.1"
                     /translation="MRSPNSTQAIQIFQVARFGALLLASICLSKSGMSVHDIGQYETC
                     MLLAGTLSFFWINGTTHTYLSQARRGRSSSLNSSLFWSVVCTSILLIGGMLLFQKPLL
                     QLFKLEIDTRFFWLLIIFFVVNNLAFIVDYYLLAADNAQGLLRLSAFHLIVQTSLIAI
                     PAFIYHDFQSAFTGAIVFVSIKAAITIFLTSRNGGLALDLAVVRKFLSDASPLILSFF
                     LGGMAIYVDGIIVTQFYDKGTFATYQYGAREFPITLLLANAFSAAMVAHISNAPSDTE
                     KVLQGTSQLMRRIFPIVIILMALSYWLYPLVYNPQFKNSYIYFNIYLLLTISRLCFPN
                     AILLGHGNSKIILRAGMIEFLLNIIVSITLLKLIGIQGVAYGTVIALMANKSILIYHL
                     HKQGVPLRTYFPVRLASIYGSVLVAVFIFFTYFIHL"
     gene            13052..14599
                     /locus_tag="PLM90_03615"
     CDS             13052..14599
                     /locus_tag="PLM90_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88486.1"
                     /translation="MRSTQRQFILWGSLLALCAAMVVVWGYTFGHENSIQLIPFALHA
                     HDPSLYDQDFFVSHAAARFPNERSFFMLLLMPFGNNLEWPSFIIYVVCSLLLFRALYG
                     IAIHLLEDPWYAFGALMLLIFPLNFHTLGLNELFGRELSTSLLSDTACAWAIWHFLQH
                     KKWSPWLLLIFATLMHPLSGLQVFLLISATLFTQSVLSRRVSQFLRMYGLQIALYMFT
                     GGAFIIALQLGLDDTQWDDATFFQTFFVFRNAHHYIPTAFPHKDWVLLGPLYLITPFL
                     LYRQSKALFIFSLWIIGGCIAYSFCVLQLHSVTVATAQWFKSTMWLEWFMIIAIMLTG
                     KTLITNIKGRSYGPAIYALTVLVSVAWLLIVFPQYRFWKINTAYELPFYEVTTPEIDI
                     SRQVKERTAKSDFLIQPAGMDELKYYSERSSFVDYKALTHTKGFIKEWSRRFELVYDI
                     DPATSKVISFDAVRLADEHFRALTPEKLRELHQTYAISHIVTFADVQLPFALLYKNEK
                     YALYQLY"
     gene            14609..15385
                     /locus_tag="PLM90_03620"
     CDS             14609..15385
                     /locus_tag="PLM90_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMZ88487.1"
                     /translation="MHYGIANQSLIPVRKSADHASEQTTQLLFGELYQVHEVRDTWIR
                     IVTHFDQYPGWIHIKQHTELTTAESDKLLKAPVSVAAEVVQIISNNARSFPVLIGSTL
                     YDFDGMNFRVGKEKFVYAGQAVSADSGLIQGEHIRRFALKFLHAPYQWGGRSPFGIDC
                     SGFTQLVFKLYGISLPRDAYQQADSGKTLHFIHEAREGDLAFFDNDEGKITHVGILLG
                     NDQIIHASGCVRIDLIDHYGIFNRDEKKYSHKLRILKRIV"
     gene            15382..16848
                     /locus_tag="PLM90_03625"
     CDS             15382..16848
                     /locus_tag="PLM90_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMZ88488.1"
                     /translation="MKILSEAQLHEWDAYTILEQDISSDMLMERAAIAFTNSLLPHLS
                     VNEKIGIVCGSGNNGGDGLCIARLLHRKGFHVHVVLYGANTSTDNQLNYHRAVKAGVR
                     IFLGENDIIPEQIQSSSLIIDALFGSGLTRPLSAINASVVEQLNALHARKFSVDIPSG
                     MMADIPSSGVVFRADHTFSFETPKLAFFFPENASVLGNWKILPIGLSESFLQKAETPF
                     HIIDHNTISSLLRQRDTFSHKGSNGHALIIAGSASTPGAAALCSFAALEAGAGLVTLC
                     SDIDYPEDPEVMITQRKNASRKIADPKINVIALGPGMGIDEEALAICKLVLQSAKQTL
                     VLDADALNILASNKELINMLPAGSILTPHPGEYARLFGAFQQWDELIIQLQQIAKSTG
                     CILLYKRAFTIVVLPDGNIWFNSTGNAGMATAGSGDVLTGMITALLAQGYNAEDAALL
                     GVYLHGLSGDIAMQYHHGQNLAAGDILDHIQDAYAFMM"
     gene            16936..17622
                     /locus_tag="PLM90_03630"
     CDS             16936..17622
                     /locus_tag="PLM90_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HMZ88489.1"
                     /translation="MKIVALTGAGISAESGIPTFRGAGGLWEGHRIEDVASPDGWYRD
                     RSLVLRFYNERRKGIKLAKPNRGHVALAELEKQHQLHIITQNIDDLHERAGASNILHL
                     HGEIFKARSERNVHITIKQEDDINLGDLATDGAQLRPHIVWFGEEVPMMEPAIQIVQT
                     ADILLIIGTSMVVYPAASLMHYTKTGIPVFVIDPNIPAITKQSNIHFIQEPAGTGVDT
                     FIREFLPIYL"
     gene            17622..18182
                     /locus_tag="PLM90_03635"
     CDS             17622..18182
                     /locus_tag="PLM90_03635"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMZ88490.1"
                     /translation="MSQSENPSNPWTTLEKKVIYDNPWIHVEEHQVINAAGNPGIYGV
                     VSFKNYAIGIIPVDNEGNTWLIGQHRYPFDLYTWEIPEGGGSIKESPLENAKRELAEE
                     AGLIADKWELIQEMQVSNSVTNEYAFIFLAQDLHPTPPNPDEDEALVIKKLHITEAYT
                     MVDKGEILDSLTVVALLRARVLGIGM"
     gene            18272..18820
                     /locus_tag="PLM90_03640"
     CDS             18272..18820
                     /locus_tag="PLM90_03640"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ88491.1"
                     /translation="MDENILDQHEVEQSSYQLAGFWDRFLASLIDGLIFIPVTGLAFY
                     NMMTLKILPLALILGLLFIIYKVYMEAARGATIGKRAMGIRVVTMDYQPINSNASLAR
                     NSLFALNAVVGLVNTVMLFNAINFENTTGFMDYITFQNANSLQLANLIGLVVFISGLW
                     IAFDKNKQGLHDKIGKTLVIKD"
     gene            complement(18821..20167)
                     /locus_tag="PLM90_03645"
     CDS             complement(18821..20167)
                     /locus_tag="PLM90_03645"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ88492.1"
                     /translation="MQHILVLGAGKSATVLIDYLLDNAAEYQWTVTIGDLDEDLAKQK
                     IKAHPQGNAVYFNADDAALCQKLIGESDIVVSLLPAFVHPVVAKICVALGKHFVSASY
                     VSDAMRALHNEAVSKGLILMNEMGLDPGIDHMSAMKIIDHVRNMGAEITGFRSYTGGL
                     VAPESDNNPWNYKFTWNPRNVVLAGQGTAKFLSNHRDKYIPYHRLFASYDIMDVPGYG
                     KFEGYANRDSLSYRSVYGLEHTGTIIRGTLRKQGFCDAWNVFVQLGMTDDTYHLEEAH
                     KHNWSDFTDAFIPSGAGSVRERLQQYLAIDNEQIMHKLDWLGLFDNKAIFTQIPARTF
                     TPAQLLQILLESKLSLDAGDKDMCVMLHVMDYSLHGKAYSLQSSMVAIGQDEVYTAMA
                     KTVGLPAAIAVKHILSGTWQHTGVVTPVHSDIYTPVLQELSDTFNIHFVETLQEFA"
     gene            20214..20693
                     /gene="cdd"
                     /locus_tag="PLM90_03650"
     CDS             20214..20693
                     /gene="cdd"
                     /locus_tag="PLM90_03650"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HMZ88493.1"
                     /translation="MDTISIQLQLTRHSGTDNFSVEEQMLIKAAHEALSSAYAPYSQF
                     QVGAALLLEDGSIVKGSNQENAAYPSGLCAERVAFFSAGSQHPGKKIMAAAVTTSYPM
                     EHPISPCGACRQVMAEYELKQQTPIKLLLCSHDGSVIVVNNSRDLLPLYFSGEWLKK"
     gene            complement(20773..24192)
                     /locus_tag="PLM90_03655"
     CDS             complement(20773..24192)
                     /locus_tag="PLM90_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HMZ88494.1"
                     /translation="MLRYMKTALCTIFLMTMLVSAVKADPGDTIKVQTFTFGSPQDAW
                     FDLPGLDVPVEKILMKYTLKCNPAQSPACGEWDYLTYTYLYEHTGVLDSTLLSHPWFL
                     ANGNAPDTFNYSSTPTFISVPSWEYYIVYDDTTSLTTAVTGISTTSTATPFDAVHPIS
                     RTQIIWTAAELTAAGLTAGDITGMDFDFSSASSSLNDLTIRIATTALTSFGANTFEGG
                     LTTVYSGDFLPGTGWSGVDFDTPFNWDGISNLIIDISYQNESTGSAATINAESTVDPM
                     VLSYAGADKYIEFHTPDQADVYGNPLSGLNDQVTISFWSMGDAPFQPQNGTTFEAITA
                     DGARVLNSHTPWSDTNVYWDAGNDGGYDRINKAATAAEYEGNWVYWTFTKNTTTGSMK
                     IYKNGVLWHSGTGKIKPMTGIDHLRLGKGNWGGSESYAGFMDEFAVWNVEMPAADIAA
                     YMYKDLDATHPYAANLQLYYQFNDGDGISEPDQSGMYDLGLIGATTHLAKATEIARNA
                     QTSVTRPIVRFEQGVFTSHIDSVLIVDAVANDPITLVYYDPADPTTATDTTLVWPTNY
                     YVYNYNVDGTVSDSTFIAATNTIYNTDLPYYSAPFEVINQYELARYITPYGIGLDLGE
                     GFTWTYDVSDYRPILHDSVHLAAGNWQELLDMELWFIEGTPPREPKKVQNLWYGFYSY
                     GLTPSYDEQTPPKTVTISADAANSRVKIRVTGHGFGGTSNCSEFCAREHYLYVDGEQV
                     WSKEVWRDDCDLNPVYPQGGTWVYDRANWCPGAEVWTYDYELTPEVSAGNTYTFDYNA
                     EAYTWNGAGSVPTYVTSVQLVTYGEPNFTHDAALDAIIAPSDDDMQSRKNPVCSDPIV
                     RITNTGSATLTSLDITYGIKDYDQDTYTWTGNLAFMESEEVTLPNFGWSPTGTAFIAT
                     VSNPNGVADEYVYNNSITTPLTMPPVYPSDIILDLKTNLASHENDLFLYDETDSIIFQ
                     RTTFTDNTTYKDTLHLDDGCYRLYLYDYGEDGLSWWANSDGSGWFRTRNIDGSYIKNF
                     GTDFGGLIYQQFTVGNYTPVEEQFTQPSDMQVYPNPADAEVFIRVALPHLSDVQLKVY
                     DITGNLIYAETQKRTQTEMFHLDVSTWSAGAYIVTAQSEDKVFNTKFVVEH"
     gene            24272..24928
                     /locus_tag="PLM90_03660"
     CDS             24272..24928
                     /locus_tag="PLM90_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ88495.1"
                     /translation="MVTATGIYKSYGPLQVLKGVDLQVNPAEVVSIVGASGAGKSTLL
                     HIIGTLDKPDKGDVILNDVSVKSLSEKQLSAFRNKLIGFVFQFHHLLPEFTALENVCI
                     PGYIAGRKESEVEARAKELLEYLRVSDRMQHKPNQMSGGEQQRVAVARALINQPAVIL
                     ADEPSGNLDTQTAKELHELFFQLRDTFKQTFIIVTHNTELADMADRKLVMQDGQMVLG
                     "
     gene            complement(24941..26122)
                     /locus_tag="PLM90_03665"
     CDS             complement(24941..26122)
                     /locus_tag="PLM90_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMZ88496.1"
                     /translation="MQAAGINRSSIQFKGKRVPVYTGSFWTSKQREGHNLHEIAYRAC
                     FKPSLPKYFIEKYTNEGDVVYDPFSGRGTTVIEAALRGRRIIANDVNPISAILSGGRL
                     CIPELQALDQQLSHILSGTPVKEQHTLSMFYHKHTLNEILLLREWLKKRKLSGTEDAL
                     DRWIRMVATNRLTGHSPGFFSVYTLPPNQAVSRERQLLINKKRNQKPEYRDVHALIMK
                     KSRNLIKDLDISTKKYLSQAATSALLINSDAAATRQIKNNSVTLVVTSPPFLDVVQYA
                     KDNWLRCWFNDIDVNEIAKRITMSKTVDAWMQKMQQVFFELYRITAKGGHVAFETGEI
                     HYGKTRLETIVLEMGLHAGFEVDRLMINEQQFTKTANIWGIRNNTHGTNSNRIVLFRK
                     K"
     gene            26237..26776
                     /locus_tag="PLM90_03670"
     CDS             26237..26776
                     /locus_tag="PLM90_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88497.1"
                     /translation="MQNTLPFGNVGDITFTPSGQDLKIYVSGQLLGTFNSSSPATLFI
                     TQNGSNKAITFKQISQSAYMQGNTGTPGVDTIYFNPLTGNSKIEACSGGAGCFKLTGI
                     GWEDDDYGQTCTGGSKGFTNLFNISAEGQLKAKFEQVSINGSMQIRLVARYSGNVMCK
                     QTPATAICCNDNYCYYCNE"
     gene            complement(26786..>27981)
                     /locus_tag="PLM90_03675"
     CDS             complement(26786..>27981)
                     /locus_tag="PLM90_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88498.1"
                     /translation="EKGLTQMRSKALKKAKELAYEYDLIYCLVTILQRERIYALKFSG
                     GDPNETIRKLHAEEEWVFDRLNNESKLGEVFFTLWVQFIKDPLMRSNEDLEQYTSSRD
                     AVLNQKFEDLHTFTEKFFWLRAKGLICKYNHDYQGTFQLTKQLVDLFSDYPQHRLNVM
                     GNYIDALSNYLSAAHVVGNYDEFEEVIAKLEELPREAVKDDNSISQVVINNKILYAMN
                     RGMYSRSGMLAEEFENYCRKFENIITESFLVVTPYNLSLMFFINGEYDRSLDYCQRVM
                     QFKTDVKIELQHGASMLYLIILIEQEQLVFFESAYRSVVRSMKNRGKFDDFERLFTTQ
                     LKKIANTPERQRKPLYGKLYEDLLVLKKESGNKRFVFMTETLSWCASKTGDKKMAELV
                     LQPFN"
BASE COUNT         7847 a         5897 c         5773 g         8264 t
ORIGIN      
        1 ggaatgtcgt gttttttttc acgcagagac gcagaggcgc agagaaatta atttgcattt
       61 aaaatttagg agcactcaat gcaataaatt ccaaaatcta attttccgtc ccactaccca
      121 ctactcacta ctcactacaa atacctgcta cctgatactt gctacctgat accatttaat
      181 aaaacagatc ccaattaatc ccaagtttaa acgatctcgg ttgcatgggg taattcgggg
      241 cttcgtagta gcctttgttg aagagacctt gggcgatgtt gtccatgcgc acgaagaagc
      301 ggaaggtgcg gatgtcgaag ctggcgaaga ggtcgatgac aggaggaaaa gtgctgctat
      361 tattggtgcc ataggaagca aattccagga agcgacctgt gatcatatca tatttcattg
      421 gtgtatatgc cgatgaaccc cagaaatgaa tacccgcttg tgaatacaat gcatttttaa
      481 ataagttgcc ttgaacatac caatcgccgg tataaataaa attcggaatg gaagtaatat
      541 ccattgcaaa ttgcgcaccg gcaacattat caaaaacaaa attgcccaat gtgatttttt
      601 tccggatata tgcctgtccg atattaaaat cgagcgtatt ggtgtgcaaa taatgctcct
      661 caaaccatgt agacgttgat gcataattgt aatccttaaa catgctaaac gaggcatgaa
      721 aggtgaaatg ctgtggtttt gattcggcat ttaatcctag cgaaaatacg gttggcatac
      781 ctgaaccatt ccattcatct ctgaatccgg aataatgtga ttcaattact gttggattca
      841 gtaatccgta gcgaacaaaa atatgcgact caggattaat ggagtcgaat tgaatgatca
      901 atttagtgtc gtaattttta ttgatcagat catatgcagc gtcaatacca agtcgtccgc
      961 cgtgtttacc aactgagggc gacagcaaat gtgtgccaat aatgaaatgg tcgtaatcct
     1021 gtttgccggc ctgatcggac aggatatgat gctgttgcaa tgcataaatt tctgcaaaca
     1081 gttttgcggg aattacacta tcaacactta ctctatgagt aagcgaggtt gcgaattgca
     1141 atttattttc aaatcctctt accgatgttc tgtcgcgcaa ggtgtcgctc tcatataata
     1201 atgtgccgta gtatgtcctg tccgttgcag gatcttcaaa taaataataa tgatctattc
     1261 cggttaaggt atgtgtgatg acaaatttgg ggtcgaaata atttcctgtg gtggaatcat
     1321 tgatctgata gctgaaattt ttcccgagtt gatatgattg cgtaatacgt ccctgccaat
     1381 tactccactc agtaaatgcc tgcgtgcgaa atggctctgc aagttccggg ttgggatagt
     1441 tgaataaggt atcattattg ataccaccat tctcctcatt attatttttc cctctaattg
     1501 ctgcgccttc gattaaatat cggttattct tggtggtata ccatcccgac aaggtagtat
     1561 ttccataatc attgcgcgaa tattgataat atcctgtaga cactaatttc gaaaaatcga
     1621 tggctacatt tgcttgaggt ccaaaattgc gtgtatgtgt tacatgaaat aattgctctt
     1681 cactttggcc ggctagataa tataaccgcg tgaaaggaac gtttgtgcga taatatttta
     1741 cttcatcgag tgtggtgata taggcatcgc gttcatggcg accgtagtca aatcccaatt
     1801 cgcgatgata atcaaaccac cgcggcgtat atggtgatcc taaatttcca agccaggcat
     1861 tttcaaataa atcttgtctg gcaggattaa acattgcgaa attatcgagt gtagtatccg
     1921 gttctaccca agtttgcatt tccgcgacgg tatgaaattg gacgcgggcg ttgtgatagt
     1981 tgtataaact gggtaacgac gtgctgtcgc ccgcaagcga atcagtttgg gcgaatgccg
     2041 ctaatgtaca caacagcaga atcgttgttg cggagatacg cctgacgatt gaactgagaa
     2101 aatggaattt cgatttcttc cccaaattat aattgcgcta tcaatttttg cagaattaat
     2161 gtcattttcg gctctgcttc ttttgccacc gctatcacat catcaacagt cacaggttct
     2221 atttcttcca taggatatcc cttgtcggtg atcacggaaa tggcaaatac gggcagcccc
     2281 atgtgcactg ccgccaacac ttcgggcacg gtgctcatac caactgcatc accgccaatg
     2341 atgtgcaaat aattgtattc cgcaggtgtc tccaaatttg gtccgggcac acttacatat
     2401 acgcctttat gacatcgaat attgttatcg cgcgcaattg acatggccgt ttcgattaaa
     2461 ttttcatcgt agcatcggaa aagatcaggg aatctcggac cgaggtcgta ttcattcgga
     2521 ccgatcaaag gatttgatgg ctgcaggttg atatgatcgc gcaatatcat taaatcacct
     2581 ttttcgatat ttgaattgag gctccctgct gcattcgaaa taaacaaacg tttgatgccc
     2641 agtaatttca tcgtgcgcac agggaatacc acttcttcca tggtatatcc ttcataataa
     2701 tggaagcgtc cctgcatggc cactactttt ttatcgccta tacttccaaa aattaattta
     2761 ccgctgtgac tttcaaccgt cgatacctga aaatgtggga tgaggtgata gtttatttca
     2821 gcaacgatat cgatatgcga aactaaattt cctaatcctg ttcccaggat gataccaaat
     2881 tccggttgaa aatctgtgcg ctggcggata aagtgcaccg cctcattcaa tctatccaat
     2941 aaagagtcca taattgaaaa tatttacaaa gataagggtt catttattcg tttgatactg
     3001 ttgtttctgt aggggaaggc gtgctttgaa caataaaatc taacactaat ttttcaaata
     3061 catctgcctt gttaataaaa actacctcaa caggcagcac aaataccttg aggccgtttg
     3121 caagaatggc ttctttttgc tccatcagac gcatggcatc cttttcggta gtaatgatgg
     3181 tatcgccgcg ttctttgata cgtttgatgt cggaaacgct gtaataatga tgatcggcaa
     3241 acgacatttt tgtgatatcg gcatacatgg ttgaaacatg tgcaaacatg tctttatttc
     3301 cggctattcc cgtcaccatt acaggttttg ttatctccaa cgatatgtcc tgatcagcaa
     3361 ataaaggata cagcgcctcg tatttcaagg tggtgaaaaa tacctgcctg tcattttggg
     3421 gtccaatctt cttcattatt tcttgctgtt gttcaaccgt aatatcgaac gggcatttgg
     3481 taacgatgat gacttgtgcc cggttttttc ctgcgcgtgt ttcgcgaagt cggcctacag
     3541 gcaataaata atcatcgtaa aatggctttt ggaaggtggt gagtacaata ttgagacctg
     3601 cacgaaggcg gcgatgctga aaaccatcgt ccagaagtat cacctgcgtt tgaggctcat
     3661 cgtgtaaaac ctggtaaata ccgccaaccc tatcgatgct cacagccacc tcaatatccg
     3721 ggaatttctg cttgtattgc ttgggttcat caccgatgtc ctctaccctg cttaattccg
     3781 tggcgatgcc aaagccggta taggtgcgtt tgtatccgcg actgagggtt gccaccttgt
     3841 acgacgattt cagtaaccgg atgagatatt cgatatgtgg tgtttttcct gtgccgccgg
     3901 cgcttaagtt gcccaccaat attacaggga aatcaaattc aaaactccgc agccaccctt
     3961 ttgtaaagaa gaaatttcgc aagcctgtca ccaatccata caagcacata aatggcaata
     4021 ataaccaacg catggggcga aaatagcaaa aagggtgcat gggaagcgaa aaagtccccc
     4081 tatagggacc cgagggcccc tacgagggaa acgaacacac aagctacttt ttgggtcaat
     4141 cgacctacat acgataccac acctctcccg gtttgagttg ttaacaaaat ttatttggat
     4201 ggtattgcgt tttatgttta ctttgcattt caaagtaatt tgaataataa tgataacaaa
     4261 tatctttttg ctgattttgg cagagtttgg tcttcgtggg ggacacgaga ggcaggggtt
     4321 gaaatcggca gaggggctta aaacaacaaa aaaggactat gaacaggatc aaaaactatc
     4381 cgcccgaccc catgcacttc gctataagcg caggattgaa gattatggtg gtaccttgtt
     4441 tttactggct ggctgtattc tcggccacat tatttgagaa accggtgtta gtgaagtggc
     4501 tgttgcttga tgacagcgta atcatgcagg cattgttgta tcccgttatg atcatgcttc
     4561 cgctcattac cctgttgatc aatattaaag ccagatatga gcggcgcatt gtcgatgcag
     4621 ttcgttcccc atttgctaac agggtgcaat ttggcgtaat gggaatggcg ttgctcagtc
     4681 tgattggtgt atttacctat acatttttcg agcgtcttga tttttggatg tacatcacag
     4741 gcaaatgaag aattatccgc ccttcggttt tgttttcaga atttggttgt ataccaattt
     4801 gttaggctcg cttttaacag gattattttt tgcactaagc gaatcaatct ccaacgattt
     4861 tttcagcagt attttaggta ctttcttcct gtttattgcc cttgcattat ttgtcgggct
     4921 atggcgaagt ttgcctgcat tgttgctgac gctagggttt gtaacattta ttgtgcctta
     4981 ttttaaaaag acaaaaccgg catttatcgc gacaattgca ttcattgaat tgctttgtcc
     5041 gcttacattc tacatttatt tcttccgcaa caggccggaa gatttatttg aaaatttact
     5101 ttttacagga ataattggag cctacatgtt atgcattctg ctgatgacca ttaaacatcg
     5161 aaaacatttt tttacacctc tgtcgttaag tgacgaccct gtaaatagcg agcaatcaac
     5221 ttcagaagaa attttaatca attcaaatca atcggatcat gtttagacag tatggatggt
     5281 tttttattcc ggcatcaata gtaggtgtag gaattgcagg tgcggcattg atattcatcg
     5341 tacaaacctt tttggtgatc gatagtcagt cgcattcagc ctccgacacc ttatatggct
     5401 ggtatccgta tgccgtgacc acctttttgg cttatcaatg gatagcgggc aatttaaagg
     5461 acaggaagtc ctaaataaac tttacatccc tttacacgaa ggatttctaa ttccgaactt
     5521 gccatagccg attaagcgta tctttgcggt caaatgcaag gatggtcatt actgatggat
     5581 ctttcaactc cctttaagga gccggttatc attttcagta ttgtattagc aattattctt
     5641 ctctctccca tcctgcttaa aactttgcgc attccttcca ttgtggtgat gatcattgcg
     5701 ggagtgataa ttggtccaca tggctttcaa ctactggagc gtgatggtgc gatagtactt
     5761 tttggaacag ttggtttact ctatatcatg ttccttgccg gactagaaat agacctgcat
     5821 ggatttagga aaaatatatc gcgaagtgtg agttttggtt tgttcacctt catcattcca
     5881 tttataatag gttttgctgt ttgtcgcatg attttgcacc tagacatgat tccggcatta
     5941 atcaccgcga gcatgttcag tacgcaaaca cttgttgcct atccgatcgt aagccgatta
     6001 ggcatcacaa gatctgcgcc tgttgcgata acagtcggcg gcactattat taccgataca
     6061 cttgtgcttt tattgttctc ggtggtgacc aatttcgcca tggataaaag tgattcattt
     6121 ttttggttgc ggcttgccat ttcagtgatc gcatttgcgg ctatcgtttt atacatctat
     6181 ccgatttttg cgaaatggtt tttcagcaaa ttggaaaatg agggtggatc gcaatttatt
     6241 tttgtgttga cattggtgtt tatagcggca tttctttcgc aattggccgg tcttgaacac
     6301 atcataggag cctttcttgc cggtatcagc ttaacgcgat tgatacctca tagttcaaca
     6361 ttaaaaaatc gcattgtatt tgtaggaaac aatattttca tccccttctt tctgatcaat
     6421 gtaggtatga tcatagatct gcacgtgttg attgaaaatg gcggggcatt gacagttgca
     6481 gcaatattgg ttactgttgc actttccaca aagtacatcg ctgcatggat aacgcaaaaa
     6541 acattcggac ttacccgcaa tgaaggcagc ctgatattcg gattaagcac agcacatgca
     6601 gcagctacac ttgccattat tttggttgga tttgaccttg gactattaga cgaaaaaata
     6661 ctaaacggca ccatcatcgt gattttggtg agttgcctgg taagctcctt tgtaacagaa
     6721 aacgccggaa gaaaatatgt gttgctcacc gcaaataagg tggatacaga ccccgaggta
     6781 atggagcgga ttttagtacc tgtcggcaat ccggattccg cagaaaaatt attagacctt
     6841 gcaatgatga tccgcaatcc cggctcgaag gaacccattt atcctttggc tgttgttgaa
     6901 gatggtccgc aagctcgtga acaattaatt cgagcaagac gaaatttaca acttgctgca
     6961 gaacatgttt catcaagtga aacgaaagta caggtattaa cgcgagtaga cgtgagcatt
     7021 gcaggtggca tttccggtgc cgtaaaagaa cttggtatca cagaagtagt attaggatgg
     7081 cattcaagag tatcgacgag cgacaggtta tttggaagta ttattcagca ggtaataaaa
     7141 agtacggatc aaatgttgtg gataggccga ctatcacaac cattaaacac aatagatcgc
     7201 atcgccattt taatgccacc aaatgctgaa ttcgaatccg gatttttcag atggctggaa
     7261 ggtgttaaac aattgtcgca gcaaacaggc gcgcgttgta tgttttgggg agaacgaaaa
     7321 acactggaaa ttgtcgatgc cgtaaataat gccaaatttc gactcatagc agaatacagt
     7381 gaatttacag attgggacga cctggagagt ttacaatcga ttgtaaaaac cggcgattta
     7441 ctcgtggttg tttcggcacg acaaaaaagt gtttctcatt tttctgcact cgatcaggtt
     7501 cccggtcgca tgcataccat atttggcgat aaaagtttta ttctgttata tccctatcaa
     7561 tcagaattcc gctcgggtga tatgaatttt gaatatagtg ttttaacacc ctctgctttc
     7621 aaagaaaata ttgcccgcat caatcaaatc ggcaaaatcg tttataaggc atttactccg
     7681 ggaaaggaga agaagtaaaa aattgtgttc atatgttcgg gcatgnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnaaatc cgtgtaatcc tttaatccta ataatccgtg
     7861 attcagacaa taattgtgtt tatgtgttcg ggcatgaaat ccgtgtaatc ctttaatcct
     7921 aataatccgt gattcagaca ataattgtgt tcggggtttg cattttgttg cgcacttgca
     7981 catggagcgc agcgacatcc cctctcagct gaattcccac ccgcctgcgg cgaggctggc
     8041 attctcacat tctcacattc ccacccgcct gcggcgaggc tggcattctc acattcccac
     8101 atggagcaca gcgacatccg cctcagcagg acttacaatc ttacacactt acacacttac
     8161 acacttgcac acctgtacac ttgcatactt gcacacttgc attcttacac acttacacac
     8221 ttgcacactt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacacttac
     8341 acacttgcac acttacacac ttgcacactt acacacttgc acacttacac acttgcacac
     8401 ttgcacactt acacacctgc acacttgcac actttaatac agcgcatttg tcatttttcc
     8461 accaattcaa tatctttgca cctccaaaac ggtgattgta gctcagctgg ttagagcatc
     8521 ggattgtggt tccgagggtc gtgggttcga gtcccatcat tcaccctaag aaggttcgac
     8581 taaataagtc ggaccttttg ttttttataa atatcctcaa ttttcgccac tcacccccac
     8641 gccaaaggca agccaaactc accctctgac cacccgccta ctcggcgagg taaactcacc
     8701 cacccgccaa aggcgaggca agctcaccaa tctcctctct cctctctcct ctctcctctc
     8761 tcctttctca tctctccttt ctcatctttc cccataccac ttactcacct gattccaatc
     8821 aatcacattg aggaaatcgg caatataatc gactcgtttg ttctggtgct tgaggtagta
     8881 agcatgttcc caaacatcga ttccaagtat gggtcgaccg cgcagagcag caacatccat
     8941 aataggattg tcttgattag gagtagtggt aatagccagg ttattttcct tatcaacaat
     9001 cagccaagcc cacccggagc caaacactgt tgaggccgat ttgctgaatt cagtcttgaa
     9061 attgtccatt gaagtccatt tggcgttgat cgccgcttct aactcagcag aagcttttga
     9121 atctttagcg gttggcgaca tcaatttcca gaacatcgaa tggttgaaat gtccgccccc
     9181 atggttgcga atggcattgc gcacggcttc aggcagttcg ttgatattcc tgatcagatc
     9241 aggtagcgcg cgtcctttga gctcaggagc tttttccagt gcctcgttga gtttggtaac
     9301 ataagcttgg tggtgctttg aataatgaat ctccatagta cgcgcatcga tatacggttc
     9361 cagtgcatca tagccataat cgagtttcgg aagctcaaaa gtgccgcctt ccgggttgaa
     9421 ctccgctaat gacggcgcgg aagccgcatg tgccgctgga aagcggaaac tgttaagaaa
     9481 gctaacactc agtgccgcca gggtgccttt gattaaaaag gaccggcgtg aattgtttga
     9541 tgaattttcc atatagtctt gtcgttattg tcaatgataa gtaaaactaa acaattggct
     9601 gtgaatacct gctcaagaag tactgttcac aaaccttaac tttacaaaca gatttacatg
     9661 caaaaatcgg gcgagaatat atcattacag gtagaaggga tgacctgctc caattgtgcg
     9721 ctaggcataa gtcggctaat tgaacaaaaa ggtgcacacg atatctatgt caattttgct
     9781 acgggtgatg ttcgctttac ccttactcct gagcaaaaac tcagcgtttt tgttgatggt
     9841 atcaatggat tgggctacaa agtaatttcc gggaatgcag aacacagcga gcggaaaaaa
     9901 aagttgtcgg atatagaaat taaatttctc atcacccttc catttacatt tcttttattg
     9961 ttgagcatgg ttccgggctt gtcggcactc caccaacctc tcgtgcaatt tctattgtgc
    10021 ctgccggttt ttgccatcgg cttttatcat tttgggaaaa gtgcacttgg ctcaatacgt
    10081 tcaggtatca tgaacatgga tgtgctcatt tttacgggaa gcaccgctgc ttttgtttat
    10141 agcttgtatg gttggtacag cgggaatttc atgagcatga tgttttttga aacgtcggca
    10201 agcataatca ccctggtatt attgggaaat atgatggaac accgcagtgt gcaacagaca
    10261 actactgcta ttcgcgccct acaaaaatta caacctgaaa ctgctaagcg tgtgcatttc
    10321 gacctgatgg caggtggaga tcatatagat gaaattccgg cagatcaatt gcaaaaaaat
    10381 gatgttatac tcgtcaatac aggcgaccgc attccgacag acggaattgt tatgcatggc
    10441 agtgcatggg tggatgaaag catgctcacc ggagaaagcg cacctgttta taaacaacaa
    10501 ggtgatgtgg tcaccggcgg aacaatttcc gtcgacggaa gtattaaagc aaaagtaact
    10561 gctctgcctg caaattcagt tttagcgtcc atcatcgaga tggttcgtcg cgcgcaagtt
    10621 gataaaccaa aattacaacg cctcgccgat cgtatctctg ccatttttgt gcctgttgta
    10681 ttaagtattg ccttgcttac gctattaata tcgcattttg cctttcacat gagttttgaa
    10741 aattcactta tgcatagcat cgcagtgctg gtgatagctt gtccttgcgc aatgggtctt
    10801 gccacaccaa ccgctgttat ggttggactt ggacgcagtg caaaaaatgg aattctcatt
    10861 aaaggtgccg ctacggcaga gacttttgct caaacacaag tgattatttt tgacaaaaca
    10921 ggaacgctca ctgaaggcga cctaaaggta agcgggatgc agcttttcga ctgtgatgca
    10981 gaatttttag gaagtctgat tcagggtctg gaagtgcatt catctcaccc aattgcacaa
    11041 tcactgcttc gacattttgc aggctcttcg aaaatgaatt tcgataatgt aacagaaatc
    11101 aaaggcaaag gtatcagcgg aaaggatgct ataggcatcg aatatcagat catcgcagca
    11161 actgcagatc aacaaccgtt tgatatagtg gtattgcgca acgataaatt aattgcggga
    11221 ttgcaattgg aagacagtat taaacaagat gctgctgaag caattgcata ttttaatgaa
    11281 cgaggtattg aaacaatttt attgagtggt gataatgcaa aaaaatgcgc tcaggttgct
    11341 gaagctacag gaatttccac cgtatatgca gcaaaatctc ctgcagaaaa actagagctg
    11401 gtaaaacaaa tcagttcaac cagaacagtg accatggtgg gtgatggcat caacgactct
    11461 cccgctttgg aatctgcaca tgttggaatt tccatgagta atgccacaca gattgccatt
    11521 caatcagcac agattattct attaaatggt catttgcaaa aattagttga tgcacataaa
    11581 cttagtcgcg ccactttaaa aacgatacgc caaaatctat tttgggcttt cttttataat
    11641 atacttgcaa ttccggtagc agccatcgga ttattgagcc ccatcattgc cgcattgagc
    11701 atggcattca gtgatgtatt cgttattgga aatgcaatac tgcttcgctc aaaaaagata
    11761 tagttcgaat gcgttcccca aattccacac aagccataca gatctttcag gtagcacgtt
    11821 tcggagcatt gctgctggca tccatctgct tgtcgaaaag tggaatgagc gtgcatgata
    11881 tcggtcaata tgaaacctgt atgctactgg caggaacgct aagttttttt tggatcaacg
    11941 gcactacaca tacctacctt tcacaggcac gcagaggtag atcttcatca ttgaacagct
    12001 cgttgttctg gtcggtggtg tgcacttcca tactattaat tggaggcatg ctcttatttc
    12061 aaaaaccgct tctgcagctt ttcaaactag aaattgatac ccgcttcttt tggttgttga
    12121 tcattttctt tgttgtaaat aatctggcat ttattgttga ttactatttg ctggcggcgg
    12181 ataatgctca gggattacta cgcttgagtg cgtttcattt gatagtacaa acatctctga
    12241 tcgctatacc ggcatttatt tatcatgact ttcaatcagc atttacaggt gccatcgttt
    12301 ttgtgtcaat taaggctgct attacaattt ttctgacttc gcgaaatggc ggacttgctt
    12361 tagatcttgc agtggttcgc aaatttctga gtgatgcatc accactcatt ctatcctttt
    12421 tcttaggagg catggctatt tacgttgatg gtatcattgt aacacagttt tatgataagg
    12481 gtacatttgc tacatatcaa tacggggcca gggaatttcc gattacatta ttacttgcca
    12541 atgcttttag cgccgccatg gtagcacata tcagcaatgc cccctccgat acggagaaag
    12601 tattacaagg caccagccag ctcatgcgca gaatatttcc cattgtaatc atactgatgg
    12661 cattgagtta ttggttgtat cctttggttt acaatccgca atttaaaaac agctatatct
    12721 atttcaatat atatttatta cttaccatca gcaggctgtg ttttccaaat gctatcttgt
    12781 tgggtcatgg taattcaaag ataattttgc gtgccggtat gattgaattt ctgctgaata
    12841 tcatagtaag tattacctta ttaaaactga tcggcattca aggcgtggct tatggcaccg
    12901 tgatcgcctt gatggcaaat aaatcaatac ttatttacca cctccacaaa caaggagtac
    12961 cgttgcgaac ttatttcccg gtgcgtttgg cgagcattta tggttcagta ttagtagcag
    13021 tatttatttt cttcacctat ttcattcacc tgtgagatca acacaacgcc aatttattct
    13081 ctggggcagt ctgcttgcgc tttgtgcagc catggtagtc gtttggggct atacgtttgg
    13141 acatgaaaac agtatacaac tgataccgtt tgccttgcat gcacatgatc cttctctata
    13201 tgatcaagat ttttttgtga gccatgctgc agccagattt cctaatgagc gttctttctt
    13261 tatgctgctg ttaatgccat ttggcaataa tttggaatgg ccttcattta tcatttatgt
    13321 agtctgttca ctgctcctgt tccgcgcact ttacggcatt gctattcact tgctggaaga
    13381 cccgtggtat gcatttggcg cactgatgtt gttgattttc ccattgaatt ttcatacact
    13441 aggtctaaat gaattatttg gaagggagtt gagcacctca ttgctgtctg atactgcttg
    13501 cgcgtgggct atttggcact tcctgcaaca taaaaaatgg tcgccatggc tgttgttgat
    13561 atttgccaca ctgatgcatc ctttaagtgg attgcaggtt ttcctgttga tcagcgcaac
    13621 attatttacg cagtcggtat tatcacgcag agtttcgcag ttcttacgaa tgtatggtct
    13681 tcaaattgcg ctgtatatgt ttaccggagg tgcatttatt atcgctctgc agttgggatt
    13741 agacgataca caatgggatg atgctacgtt ttttcagaca ttttttgttt ttcgcaacgc
    13801 acatcattat ataccaacag catttccgca taaggattgg gtgctacttg gaccattgta
    13861 tctcattact ccgttcttat tataccgtca atcaaaagca ctctttatat tttcactttg
    13921 gatcatcggc gggtgcatag cttatagctt ctgcgttttg caattacata gtgttaccgt
    13981 tgcaactgct caatggttca aatcgaccat gtggttagaa tggtttatga tcatagccat
    14041 catgcttaca ggtaaaaccc tgatcacaaa tatcaaaggt cgaagctacg gcccggcaat
    14101 ttacgcactg actgttctgg taagtgttgc ctggttattg atcgtatttc cgcagtatcg
    14161 tttttggaaa ataaatactg cctacgaatt acccttttat gaagtaacta caccggaaat
    14221 cgatatcagc agacaagtaa aagagcgcac ggcaaagtca gattttctga tacagcctgc
    14281 cggcatggac gaattaaaat attatagtga acgcagcagt ttcgtagact acaaagccct
    14341 cacacatacc aaaggtttca tcaaagaatg gtcgcgacgc tttgaactgg tgtatgatat
    14401 tgatcccgcc accagcaagg taatcagctt cgatgccgta agacttgccg atgagcattt
    14461 cagggctctg actcctgaaa agctgcgtga actgcatcag acctatgcca tttcacatat
    14521 cgttaccttt gcagatgtgc aattgccatt cgcactgtta tataaaaatg aaaaatacgc
    14581 actgtatcaa ttatattaat accctattat gcattacggt atagcgaacc aaagtctaat
    14641 tcctgtcaga aaatcggcag accacgcatc ggaacaaacc acacaattat tgtttggcga
    14701 attgtatcag gtgcatgaag tccgcgatac ctggattcgc attgttacac atttcgatca
    14761 atatccgggt tggattcaca tcaaacaaca taccgaactt acaactgcag aaagtgataa
    14821 attactgaaa gcaccggtat ccgttgccgc agaagttgtt caaatcattt caaacaatgc
    14881 acgtagtttt ccggttttaa ttggcagcac attatatgat ttcgatggta tgaattttcg
    14941 tgtaggcaaa gaaaaatttg tttatgccgg tcaagccgtc agtgccgata gtggattgat
    15001 tcaaggagaa catatccgac gatttgccct caagttttta catgctccct atcaatgggg
    15061 cggaagaagt ccttttggaa tcgattgctc cggatttaca caactcgtat ttaaattata
    15121 tggcatttct ttgcctcgcg atgcatacca acaagccgat agcggtaaaa ccttacactt
    15181 tattcatgaa gcaagagaag gcgatctcgc attttttgat aatgatgaag gtaagatcac
    15241 gcatgtagga attttactcg gcaatgatca aattattcat gcatcaggtt gcgtgcgcat
    15301 agatctcatc gatcattatg gcattttcaa tcgcgacgaa aagaaatatt cgcacaagct
    15361 tcgcatcctc aagcgcattg tatgaaaata ctatcggaag cacaattaca cgaatgggat
    15421 gcttacacca ttcttgaaca agatatcagt agtgatatgt tgatggaacg cgctgcaatt
    15481 gcatttacga attccctgct tccacatctt agtgttaacg aaaaaatagg tattgtatgt
    15541 ggatccggca ataatggcgg cgatggtctt tgcattgcgc gattgctgca tagaaaagga
    15601 tttcatgtgc atgtggtttt gtatggcgcg aacacaagca ccgataatca actcaattat
    15661 catcgcgcag ttaaagcagg tgtgcggatt tttttggggg aaaatgatat tattcctgaa
    15721 cagatacaat cttcgtcgct cataatagat gctcttttcg gatcagggtt aacgaggcct
    15781 ctctccgcga taaacgcttc tgtcgttgaa caactaaatg cactgcatgc gcgaaaattt
    15841 tcagtggata ttccttcggg tatgatggct gatatacctt catcgggagt agtttttcgt
    15901 gccgatcata cattcagctt cgagactcca aaacttgcat ttttcttccc tgaaaacgca
    15961 tcagtactcg gcaattggaa aatacttcct atcggtttat ctgaatcatt tttacaaaag
    16021 gcagaaactc catttcatat tattgatcac aacacgatca gttcactatt acgtcagcgc
    16081 gatacatttt cgcataaagg aagtaatgga catgcactta taattgcagg aagtgcctca
    16141 accccgggag ccgcagcctt gtgcagtttc gcagcgttgg aagccggtgc cggattggtt
    16201 accctatgca gcgatatcga ttatccggaa gatcctgaag tgatgatcac gcagcgcaaa
    16261 aacgctagcc ggaaaattgc tgaccctaaa ataaatgtta ttgcgcttgg tccggggatg
    16321 ggtatcgatg aggaggcatt agctatttgc aaactggttt tacaatcggc aaaacaaaca
    16381 cttgttcttg acgctgatgc attaaatatt ttagcatcga ataaagaatt aataaacatg
    16441 ctgcctgccg gaagcatatt aacaccacat cccggagaat atgccagatt gtttggcgca
    16501 tttcaacaat gggatgaatt gatcattcaa ttgcagcaaa tagccaaatc aaccggttgc
    16561 atcttgttat ataaacgcgc atttaccatt gtggttttac ctgatggcaa tatatggttc
    16621 aacagtaccg gaaatgcagg aatggcaact gccggcagcg gagatgtact tacaggaatg
    16681 ataacagctc tgcttgctca gggatacaac gctgaagatg cagccttgct gggagtctat
    16741 ctgcatggcc tcagcggcga tatagctatg caatatcacc atggacaaaa ccttgctgcc
    16801 ggagatatct tagatcatat tcaggatgcc tatgcattta tgatgtaaat gtggcagatt
    16861 atttgatctg aacctgttcc ttcagaataa aatatataca tttctcattt ttttgctttt
    16921 atttattttt actcaatgaa aattgtcgcc ttaaccggag caggtatcag tgccgagagt
    16981 ggtattccaa cattcagggg tgccggaggc ttgtgggagg gacatcgaat agaagatgta
    17041 gcttctccag atggatggta tcgcgacagg tcgcttgttt tgcgttttta taatgaacgt
    17101 cgcaaaggaa tcaagcttgc aaaacccaat cgcggacatg tagctttagc agagttggaa
    17161 aaacaacatc agttgcatat catcactcaa aacattgacg acttgcatga gcgcgcagga
    17221 gcgagcaata ttttgcattt acatggagaa atattcaaag cacgcagtga acgcaatgtg
    17281 catattacca tcaaacagga agacgatatc aatctcggtg atcttgccac tgatggtgcg
    17341 cagttgcgac cacatattgt atggtttggt gaagaagttc ctatgatgga acctgctatt
    17401 caaatcgttc aaactgccga tatattattg atcatcggca caagtatggt tgtttatcct
    17461 gccgcatcac tcatgcatta tactaaaaca ggaataccgg tttttgttat cgatccgaat
    17521 attccggcaa taacaaaaca atcaaatatt catttcattc aagaacctgc cggaacaggc
    17581 gttgatacct ttattcgcga gtttctgcca atatatttat gatgtcgcaa tctgaaaacc
    17641 cttccaatcc ttggacaaca ctggagaaaa aagtcatcta cgataatccc tggatacatg
    17701 ttgaagagca tcaagtgatc aatgctgcag gaaatcccgg catttatggt gttgtttcgt
    17761 ttaagaatta cgctattggt ataatacctg tagataatga aggcaatacc tggctcatcg
    17821 ggcaacatcg atatcctttt gatttgtata cctgggaaat tccggaaggt ggcggttcga
    17881 taaaagaatc accgctcgaa aatgccaaac gtgaactcgc cgaggaagca ggcctgattg
    17941 ccgacaaatg ggaattgata caggaaatgc aagtgagcaa ttctgtaaca aatgaatatg
    18001 catttatttt tcttgcacaa gacctccatc ccactccacc aaatccggat gaagacgaag
    18061 ccctggtaat caaaaaacta catattacag aagcttacac catggtcgac aaaggtgaaa
    18121 tcctcgattc ccttaccgtt gtagccttgt tgcgggcaag ggtgttgggt atagggatgt
    18181 agattttctt gttaaaaaat gttcatccac aagacctcat ttgcatggtc cgcaatattt
    18241 cattattatt gagaaacaaa acacaattac catggacgaa aatatacttg accaacacga
    18301 agtagaacaa tcctcttacc aattagcagg tttctgggat agatttctgg catcattgat
    18361 tgatggattg attttcatac ctgtaacagg tttggctttc tataatatga tgacgctaaa
    18421 aatattgcca ttggcattaa ttcttggttt gcttttcatc atatataagg tgtacatgga
    18481 agctgcacga ggtgcaacaa ttggcaaacg cgccatgggt attcgcgtag taacaatgga
    18541 ttatcagcct attaattcta atgcttcgct tgccagaaat tctttatttg ccctgaatgc
    18601 cgtagtaggt ttggttaata cggttatgtt gttcaatgcc atcaattttg aaaacacaac
    18661 aggctttatg gattacatta ctttccaaaa tgccaacagc cttcagcttg caaatttgat
    18721 tggattggta gtatttattt ccgggttatg gatcgccttc gataaaaaca aacaaggctt
    18781 acacgacaaa atcggtaaga cgcttgtaat taaagattaa tcaggcgaat tcctgtagcg
    18841 tttctacgaa gtggatatta aatgtatcag acaattcttg taacacagga gtataaatat
    18901 cgctgtgtac cggagtcacc acaccagtat gttgccatgt tcccgagaga atatgtttta
    18961 cggcaatcgc agccggtagt cctacagttt tagccattgc cgtatatact tcatcctgac
    19021 ctattgccac catacttgat tgcagtgagt aggccttacc atgcaaggaa taatccataa
    19081 catgtagcat cacacacata tctttatcac cggcgtccag cgataatttg ctttcgagta
    19141 aaatttgcaa aagttgcgca ggcgtgaatg tgcgtgcagg aatttgggtg aaaatagctt
    19201 tgttatcaaa taatcccagc caatcgagtt tgtgcatgat ttgctcgtta tcgatagcaa
    19261 gatattgctg caaacgttcg cgaactgacc cggctccgga tggaataaat gcgtcagtaa
    19321 aatctgacca gttatgttta tgcgcttctt ctaaatgata agtatcatca gtcattccca
    19381 attggacgaa tacattccat gcatcacaaa atccttgttt gcgcaaagtt ccacgaataa
    19441 tggttccggt atgctctaag ccataaacgc ttcgataaga tagactatcg cgattcgcat
    19501 aaccttcaaa ttttccatag ccgggcacat ccataatgtc gtatgaagca aataatcggt
    19561 gatatggaat atacttatcg cgatgattgc tcagaaattt tgccgtcccc tgccctgcca
    19621 gcaccacatt tcttggattc caggtaaatt tataattcca gggattatta tcactttcag
    19681 gtgcaacgag accaccggta tatgagcgaa atcctgtaat ctcagcaccc atattccgca
    19741 catgatcaat tattttcatg gcactcatgt gatcgatgcc gggatctaaa cccatttcat
    19801 tcattaatat caatccctta gaaacagcct cattatgcaa cgcacgcata gcatcgctga
    19861 cataggatgc agatacaaag tgttttccta aggcgacaca gattttagca acaacaggat
    19921 gcacaaatgc cggcaataat gagactacga tatcagactc accaatgagt ttttgacata
    19981 atgcggcatc atctgcattg aaatacacag catttccctg cggatgtgct ttgattttct
    20041 gctttgccaa atcctcatca agatcgccaa ttgtcactgt ccattgatat tcggctgcat
    20101 tgtcgagcag ataatcgatg agcactgttg ccgacttccc ggctcctaaa acaagtatat
    20161 gctgcataat tgagccgtaa aattactact ttcacccata gaatcaggtg accatggata
    20221 ccatcagcat acaattacag cttaccagac atagtggaac cgacaatttc agtgtcgagg
    20281 aacaaatgct cattaaggct gcacatgagg ctttgagttc agcttatgca ccctattcgc
    20341 aatttcaggt aggtgccgca ttgttattgg aggatggcag tatagtgaaa ggaagcaatc
    20401 aggaaaatgc agcatatcca tcaggattat gtgctgagcg tgttgctttc ttcagtgccg
    20461 gaagccaaca tccggggaaa aaaataatgg ccgctgcagt aactacctct tacccgatgg
    20521 aacatccgat ttctccttgt ggcgcttgcc gacaggtaat ggcggaatac gaattgaagc
    20581 aacaaacccc aataaaactc ttgttatgca gtcatgatgg ttctgtgata gtagtaaaca
    20641 actcacgcga tttgcttcca ctctattttt caggcgagtg gttgaagaaa taaatcagca
    20701 tattgcaaaa caaaaaatgg caggaattat taactcctac cattttatta taagatttat
    20761 ttaatctatt tcttagtgtt caacaacaaa cttggtgtta aacactttgt cttccgattg
    20821 cgctgtaact atgtatgctc ctgccgacca tgtgctcaca tccaggtgga acatttcagt
    20881 ttgcgtgcgt ttttgtgttt cagcatatat caggtttcct gtgatatcat acactttcaa
    20941 ttgcacgtcg gaaagatgtg gtagtgccac gcgaataaac acttctgcat ccgcaggatt
    21001 tggatatacc tgcatgtcgg atggttgagt gaattgctct tcaaccggag tataattgcc
    21061 taccgtaaat tgctgatata tcaatccacc gaaatcagtg ccgaaatttt ttatatatga
    21121 gccgtcgata ttgcgtgtgc ggaaccatcc gctgccatca ctgtttgccc accagcttaa
    21181 accatcttca ccataatcat ataaatataa ccggtaacaa ccatcatcta aatgcaaggt
    21241 atccttgtat gtagtgttat cggtgaaggt tgtacgttgg aaaattatgc tgtcggtctc
    21301 gtcataaagg aacaaatcgt tttcatgaga agcaagattt gtttttaaat ccaaaataat
    21361 atcactggga taaacaggtg gcattgtcaa tggtgtagtg atagagttgt tatatacata
    21421 ttcatcggca acaccatttg gattactaac ggttgcaata aatgctgttc ctgttggcga
    21481 ccatccgaaa tttggcaagg tcacttcttc actttccata aatgccaggt tgccggtcca
    21541 ggtataggta tcctggtcgt aatctttaat tccataagtg atatcaagtg atgtcagcgt
    21601 tgcagatccc gtattggtaa tgcgcacaat tggatcactg cacaccggat ttttacgcga
    21661 ttgcatgtca tcatcagatg gtgcgataat ggcatcgagc gctgcatcat gtgtgaagtt
    21721 tggttcgcca taggtgacaa gttgaactga agttacatat gtaggaacac ttcccgcccc
    21781 attccaggta tatgcttccg cattataatc aaaagtatag gtatttccgg ctgaaacttc
    21841 aggtgtcaat tcataatcat aagtccacac ttcagctccc gggcaccagt ttgcgcgatc
    21901 gtaaacccat gttccaccct gaggataaac cggattcaaa tcacaatcgt cgcgccatac
    21961 ttctttgctc catacctgtt ctccatcaac atataaataa tgttcacgcg cacagaattc
    22021 agaacaatta gaagtacctc caaagccatg tcctgtaacg cggatcttta cccgtgaatt
    22081 cgcagcatct gcggaaatgg ttactgtttt tggaggtgtt tgttcatcgt atgatggcgt
    22141 aagtccataa ctgtaaaatc cataccataa attttgaact tttttaggct cacgaggtgg
    22201 cgttccttca ataaaccaca attccatatc taataattcc tgccagttac ctgcagcaag
    22261 atgtacagaa tcatgtaata tcgggcggta atcgctgaca tcataagtcc aggtaaatcc
    22321 ttcaccaagg tcaagtccta ttccataagg agtgatatat cgcgcaagct catattgatt
    22381 aattacttca aacggagcac tgtaataagg taaatcagta ttgtagatag tatttgttgc
    22441 agcgataaat gttgaatcac ttacggttcc gtcaacatta taattgtaaa cataataatt
    22501 tgtcggccat actaatgtag tatctgttgc agtagttgga tcagcaggat cgtaatacac
    22561 aagagtgatg ggatcattgg caacagcatc aacaattaac accgagtcaa tatgagaagt
    22621 aaatactcct tgctcgaaac gcactatcgg tcgggttacg gaggtctgcg catttcgtgc
    22681 aatttccgtt gcttttgcaa gatgcgtggt tgcacctatc aatccgagat cgtacatacc
    22741 tgattgatcg ggctcggaaa ttccatcgcc atcattaaac tgataataca attgaaggtt
    22801 agctgcatat ggatgtgtgg catcaagatc tttatacatg taagctgcga tatctgctgc
    22861 aggcatttca acattccaaa cggcaaattc atccataaat ccggcataag attcagaccc
    22921 accccaattt ccttttccca aacgcaaatg atcgattcct gtcatcggtt taattttccc
    22981 tgtacctgaa tgccagagca cgccattttt ataaattttc atgctgccgg ttgtagtgtt
    23041 tttggtgaat gtccaataca cccaattgcc ttcatactcg gccgctgtag cagctttatt
    23101 gatacgatca taacccccgt cgtttccggc atcccaatat acattggtat cgctccaagg
    23161 ggtgtgacta tttaatacac gcgcaccatc agctgttata gcttcgaagg tagtaccgtt
    23221 ctgtggttga aatggagcat cgcccatact ccaaaatgat atggtaacct gatcattcaa
    23281 tccggacaat ggatttccat aaacatcggc ctgatcgggt gtatgaaatt ctatgtattt
    23341 atcggcaccg gcatagctca gcaccattgg gtctacagtg ctttcagcgt ttattgttgc
    23401 agcgcttccc gttgactcat tttgataaga aatatcgatg atcagatttg atataccatc
    23461 ccaattaaag ggcgtatcaa aatcaacgcc tgaccagccg gttccgggta aaaagtctcc
    23521 tgaataaact gtagtcaatc caccctcgaa agtattcgcc ccaaatgatg ttaaagctgt
    23581 tgtcgcgatg cgaatggtaa ggtcattcaa agatgaagaa gcagagctga aatcaaaatc
    23641 catccctgtg atatctccgg ctgtaagtcc tgcagcagta agttcagctg ccgtccatat
    23701 aatttgtgtt ctggaaatcg ggtgcactgc atcaaatggc gttgccgtag atgtagtcga
    23761 tattccggtt accgccgttg taagcgaggt ggtatcgtca tacactatat aatattccca
    23821 cgatggaaca gaaatgaatg ttggcgttga agaataattg aaagtatctg gagcattgcc
    23881 attcgcaaga aaccacggat gcgatagcag cgtgctgtcg agtactcctg tgtgttcata
    23941 taagtaagta taagtaagat aatcccattc accacatgca ggactttgtg caggattgca
    24001 tttgagcgta tatttcatga gtattttttc aacgggcaca tccaatccgg gcagatcaaa
    24061 ccaggcatcc tgcggagatc caaaggtgaa ggtttgaact ttgatggtat ctccgggatc
    24121 agcctttaca gcagatacca gcatggtcat caaaaaaatt gtgcatagcg ccgttttcat
    24181 gtatcgcaac atggttgtaa gtttggcaca aatataggaa agcattgacg cattagtcgg
    24241 cgctgaattg tcataactca atcatacaaa cttggtaaca gccaccggta tttataaatc
    24301 ttatggccct ttacaggtcc tgaagggtgt agatttgcag gtaaaccctg cagaagtagt
    24361 tagtattgta ggtgcttcgg gagcgggtaa aagcacgctg ttacacatta ttggcactct
    24421 cgacaaaccg gataaaggcg atgtaatatt gaacgatgtc tcagtcaagt cactctccga
    24481 aaagcaactt tcagcattca gaaataagct cataggcttt gtatttcaat ttcaccacct
    24541 gcttcctgaa tttaccgcct tggaaaatgt gtgtatcccc ggatatattg caggcagaaa
    24601 ggaatcagaa gttgaagcca gagcgaagga attgctcgaa tatttgcgcg ttagcgacag
    24661 gatgcaacac aaacccaatc aaatgtcggg cggtgaacaa caacgtgtag ccgttgcaag
    24721 agccttgatc aatcaacctg ctgtaatact tgctgatgag ccttcaggta atttagacac
    24781 acaaacagca aaagaattac acgaattgtt tttccagttg cgcgatacct tcaaacaaac
    24841 atttatcatc gtaacacata ataccgagtt ggcagatatg gccgacagga aactggtgat
    24901 gcaagatgga caaatggtat tgggctgagg taagctgatc tcattttttt ctgaaaagta
    24961 ctatcctgtt cgaattcgta ccatgcgtat tattgcgaat tccccaaata ttggctgttt
    25021 tggtaaattg ttgctcatta atcatcaatc tatcaacttc aaaaccggca tgtaatccca
    25081 tttcaagcac aatggtttcc agacgtgttt tcccgtaatg aatttcacct gtttcgaaag
    25141 ccacgtgtcc tcctttggca gtaatgcgat ataattcgaa gaatacttgt tgcattttct
    25201 gcatccatgc atcaactgtt ttcgacatgg tgatgcgttt ggcaatttca tttacatcga
    25261 tatcgttaaa ccaacagcgc agccaattgt ctttggcata ttgcactaca tcgagaaatg
    25321 gcggtgaggt aacaacaagt gtaacggaat tgttttttat ttgtcgtgtt gctgcagcat
    25381 cactattgat taaaagagct gatgttgctg cctgagaaag gtattttttt gttgaaatat
    25441 ccaggtcttt tatcaggttg cgcgactttt tcatgattag tgcatgcaca tcgcgatatt
    25501 ccggcttttg gttgcgtttt ttattgatga gcaactgcct ttcgcgcgat actgcctgat
    25561 ttggaggcaa tgtataaaca gaaaaaaatc cgggcgaatg acctgtaagt ctgtttgtgg
    25621 caaccatgcg aatccatcgg tcgagagcgt cttcagtgcc cgaaagtttg cgctttttta
    25681 accactcccg caatagcaga atttcattta atgtatgctt atgataaaac atactaagtg
    25741 tatgttgttc ttttacagga gttcccgata aaatatgact taactgctgg tccagagcct
    25801 gtaattccgg aatacataat ctcccacctg ataatatggc actaatggga tttacatcat
    25861 tggcaataat tcgccttcca cgcagggcag cttcaattac cgttgtacct cttccgctga
    25921 atggatcata aaccacatca ccttcattgg tatatttttc aataaaatat ttaggcaacg
    25981 atggcttaaa gcaggctctg taggctattt catgcaggtt atgaccctcc ctttgtttag
    26041 aagtccaaaa agagccggta tatacaggaa cccgtttccc tttgaattga atggatgatc
    26101 tgtttatacc tgcagcttgc atgagccgta aaattcacca aaaacagtga atatacatga
    26161 acatatgcac tgtggactgt cctgtatttg gttgtaataa aaaaaactgc cagttttgcg
    26221 tcatcaacca ttgaccatgc aaaatacact tcctttcggt aatgtcggtg acattacatt
    26281 tacaccttcg ggacaagacc ttaaaattta tgtcagcgga cagttgctgg gaacttttaa
    26341 ttctagcagt cctgcaacct tgtttattac acaaaatggc agcaacaagg caatcacctt
    26401 caaacagatt tcgcagagtg cttatatgca gggtaacacc ggcactccgg gagtagatac
    26461 catttatttc aacccattaa caggaaattc aaaaatagaa gcctgctcag gtggtgcggg
    26521 atgttttaag ctgacgggaa tcggatggga ggatgacgac tacggacaaa catgtacagg
    26581 aggcagcaaa ggcttcacaa acctgtttaa catttcagcg gaaggtcaac tcaaagctaa
    26641 gttcgagcag gtatccataa acggaagcat gcaaattcga ttggtagcca gatacagcgg
    26701 taacgttatg tgcaaacaaa cacctgctac agccatatgc tgtaatgata actactgtta
    26761 ttattgtaat gagtaaatgg aaatattaat taaatggttg aagcacaagt tcagccattt
    26821 ttttgtcacc ggttttagaa gcacaccacg acaaagtttc tgtcatgaaa acaaagcgct
    26881 tgtttccgga ttcctttttc agcactaaca aatcttcgta aagcttacca tatagcggct
    26941 tgcgttgtct ttccggagta ttggcgattt ttttcagctg agtagtaaat agtcgctcga
    27001 agtcatcgaa ctttccgcga tttttcatgc tgcggacaac acttctgtaa gctgattcaa
    27061 agaacacaag ctgctcttgt tcaatcaata tgatcagata aagcatggaa gcgccatgtt
    27121 gtaattcaat tttcacatcg gttttaaatt gcatgacgcg ctgacagtaa tccagggatc
    27181 tgtcgtactc gccattgata aaaaacatca acgaaaggtt atatggcgta acaacaagga
    27241 aggattctgt aataatattt tcaaacttgc ggcaataatt ctcaaattct tctgccagca
    27301 taccacttcg gctgtacata ccgcgattca tggcatagag aatcttatta ttgataacca
    27361 cctgcgaaat ggaattatca tctttcactg cttcacgagg caattcttca agctttgcta
    27421 tcacctcttc aaattcatca taattaccta caacatgcgc ggccgacaaa taattagaaa
    27481 gcgcgtcaat ataatttccc attacattga gcctgtgttg cggatagtcg ctgaatagat
    27541 ctactaattg ctttgtcagc tgaaaagtgc cttgatagtc gtggttatat ttacaaataa
    27601 gccccttcgc cctcagccaa aagaattttt ctgtaaacgt atgcaggtct tcaaattttt
    27661 gattcaatac tgcatcacgt gatgaagtat attgttcaag gtcttcatta ctgcgcatca
    27721 atggatcttt gataaactga acccataaag tgaagaacac ttcaccaagc ttagattcat
    27781 tatttagtcg atcaaatacc cattcctctt cagcatgtaa ttttcggatc gtttcattcg
    27841 gatcgccacc ggaaaatttc aaggcgtaga tgcgttcgcg ttgcaaaatg gtaaccaagc
    27901 aatagatcaa atcatattca taggcgagtt ctttggcttt ttttaaagct tttgatcgca
    27961 tttgtgtcaa tcccttctct g
//
LOCUS       DAOQOU010000024        26997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_822_length_26997_cov_6.965222, whole genome shotgun sequence.
ACCESSION   DAOQOU010000024 DAOQOU010000000
VERSION     DAOQOU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26997
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_822_length_26997_cov_6.965222"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..179
                     /locus_tag="PLM90_03680"
     CDS             12..179
                     /locus_tag="PLM90_03680"
                     /inference="COORDINATES: protein motif:HMM:NF039584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88499.1"
                     /translation="MIEENVVSAAGAPHRFTYKSIQEAIREAGFEPQLRTQQYEDREI
                     PANIQEQVINY"
     gene            250..825
                     /locus_tag="PLM90_03685"
     CDS             250..825
                     /locus_tag="PLM90_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HMZ88500.1"
                     /translation="MTNDYISSVKNQFEYYKMLGEKTFDQVTDEQLFWKFNEESNSIA
                     TIVKHLSGNMLSRWTDFFTSDGEKEWRKRDEEFENDIQNREDMLKVWNEGWNCLYHVI
                     DHLTADDLEKIIYIRNQGHSVVEAINRQLAHYPYHVGQIVFIGKMVMDEKWKSLSIPK
                     GNSKAFNKEKFDKPKERRHFTDEYLNKDDGK"
     gene            928..1578
                     /locus_tag="PLM90_03690"
     CDS             928..1578
                     /locus_tag="PLM90_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88501.1"
                     /translation="MNSSFGYIYVFIIVCSIGCTRTSNETKSKTLVNSNISEDVDSSS
                     GSDSLDVIEFIKVFALDRADTFIVGDVNGDRKVDKAIIEPATFIYRDGKVDSQYVNIS
                     FTTDIPVIRHYNGFHGLIANVGDLDGNKTDELIYYPDWFIGNMASIYIYGLRDGEWKL
                     FADGSQRRDIIFETKDPFKYLQSRVEKIDNSSFKLTEITMNENAEFIDSTSIVFIH"
     gene            1603..2457
                     /locus_tag="PLM90_03695"
     CDS             1603..2457
                     /locus_tag="PLM90_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ88502.1"
                     /translation="MKLSVKLFLHIFLFCSSFLMAAQDSTFSVITFNLRYDNPADAEN
                     NWHYRKQEIADFIQLRSPEIFGIQEGLIHQLAFLDSALTRYQYVGKGRDDGAQKGEFS
                     AIFFDTSKVTLIYDSTFWLSLTPSEPSVGWDAALPRICTFARFVTKNSGDTLNVWNMH
                     LDHMGITARYKSIALLVVMINDFDKALWLQPMIIMGDLNSTPLDDPAHTMLKFAEDTR
                     TATLTPPLGPDATFNGFAPLNTESKRIDYIFVRNLTTIAQEHIDTKRSNGLWLSDHLP
                     VMATLSFK"
     gene            2464..3732
                     /locus_tag="PLM90_03700"
     CDS             2464..3732
                     /locus_tag="PLM90_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88503.1"
                     /translation="MKLHSIRLSLLICLITFGINYMQACSMSKITFNGVTYVGNNEDS
                     WHTDPRIWFEPGAGSKRGACYVGYADKWPQGGMNIDGLVYDAFTMYDGNVQPNGKPIA
                     ENLPIFLRDIMQSCGTVDEVYKYAINFDRHNFDRAMMLFVDTTGKYLVMEADTMIIGN
                     DSTFLLANFSFSKTKDLSTVSLGRYQRGRTFLEKHEIGGLDFCRALMDTMSECRERKG
                     DGTLYTSLYDTKRRLISLCFYHNYDRVHILSLNDELAKGEHWIAMETLFPKNEEFETL
                     KSYITPANHITLLPIMLIVVILLLGMAAILSTQWFRQLHLAEKNLQYLRISIAIFNAL
                     LAIYIWVIMNEPAVYYFPAPYFEAGRGWINLLSYMPVLTLIVMAATGWLVFRKQVVTT
                     TGARVGFYVNAVVWLLCAIAFAYWGLIAII"
     gene            3846..4496
                     /locus_tag="PLM90_03705"
     CDS             3846..4496
                     /locus_tag="PLM90_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88504.1"
                     /translation="MKKLIIACMVLCTITVKAQSLESGTLSFQVGYDAMIHGTASETT
                     FFGVVVDRDTSGAVTSMFNADAQYSFAPWFSAGVAFNIGSYLEDTADQSNDGNHVSII
                     AFDARLYPVNNDKFNWYLGGSAGLTNLEINKKDALTPGVNYQYKYKSPALGLFTGFNW
                     YFFDVAGVFLQFEYSQQNFDLKSFSIDGAEQDLSQTTQSLITKGGGIRLGVNFKLN"
     gene            4599..5762
                     /locus_tag="PLM90_03710"
     CDS             4599..5762
                     /locus_tag="PLM90_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ88505.1"
                     /translation="MRFLSADVIYPSYGNPLREHVLIVQHDGRIDGIVAKADIDPLKI
                     EHFDGALCPGFVNAHCHLELSHLLGKFPMKTGLPAFLMEVTRQRETAMEDIISAMQEA
                     DSSMWQNGIVAVGDISNAPHSLDIKADSRIYYHTFVELLALDPQKATTAIEHGNAIVA
                     LAKERNLSASLAPHAPYTISTLLVEGITRACRDMGMPTSIHMLESNDENEFYVQATGG
                     YRKLYRELGIPITHFEPTGKTSLESILPFMDKDVKTLLVHNTIATVWDADWAENLHAN
                     LYWCFCPNANLFIEDRLPDIPELMQHIKHIAIGTDSLASNHGLSILDELKTIAHTFPS
                     LTTEELLQWATDGGAQFLGLSDRFGSLKPGLSPGILHLITSEIQPETLVNRLF"
     gene            5893..6570
                     /locus_tag="PLM90_03715"
     CDS             5893..6570
                     /locus_tag="PLM90_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ88506.1"
                     /translation="METFKQFLVSSVSCQTFAPPMNEEVKKILNRCERLFMKYGIKSV
                     TMDDVARELGVSKKTLYQYFENKEQLIQTVTENHFKCQNEMVQQVIHHSKTAIDEMYS
                     IAAWMNAMSKNLNPALLYDLRKYHPESWQVFIDHRNNEVLNSIRHNLERGINEGLYRA
                     DLDIEVIARIYITRVEMFIDNEIFPLDKFSPERTFNVFIDYHLRGIATPKGIKYLEQL
                     KSQNHGI"
     gene            6560..7879
                     /locus_tag="PLM90_03720"
     CDS             6560..7879
                     /locus_tag="PLM90_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ88507.1"
                     /translation="MAYKFKVLCMAVLLLPAILQAQTMRFSLQEAIDYGLKNNPQIKS
                     AELDVQNAEITVDNRIASGLPQVDAKLDYQNFFVLPTSLIPGEFFGGEPGTFIPVQFG
                     TEQNMTAGISASQLLFNGAWLVGVSAAKQYAALVQTQSKIALSDVHKNVESAYNSVLV
                     AQEFEKLLQKNIGNLDKVLFEVTELNKQGFVEGIDVDRLTISKTTLQGQLDNAKRQTA
                     LAAQYLKFSMGLDMSTAIELTDTLAPIKEVNMAGSNYLARPEFAVMESLVQLNALNIK
                     VNKAMYLPSLVLYGSYSQNAQRNEFDFLSGDGQWFETGLVGLTLSVPVFSGMQRRSNV
                     QMAQVAYEKAKLDQFTATQGILLEVEKAKTDYMNAANDLEVQKKNIALAQKIYDTALI
                     KYREGVGSSLELSTAESTLYQAQGSYIGALFNVLNTQTSLNKALGNY"
     gene            7892..9007
                     /locus_tag="PLM90_03725"
     CDS             7892..9007
                     /locus_tag="PLM90_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ88508.1"
                     /translation="MKKNFIYLAMALFIAACSPKESGDKKAELEALKKERTALDKQIK
                     ELEAEIAKTDTTKSKDPELVVVTEIQPQEFTHYIEIQGKIDADENVLVTPEMPGVIKA
                     VYVVAGQSVSKGQLLAELDNDAMRKNIETLESQLGFAKEMYNKQKALWDQKIGSEVQY
                     LSAKNNVETLEKSIAAAREQLNMSKLTSPISGLVDNVDLKAGQIGSPGFQGIRVVNMS
                     SLKAKGEVSESHSNVVSTGDNAKIIITDLNKEIDTKVSFAARVINPQSRTFSVEARIT
                     GESDLKPNMIAVIKIADYEAKEAMVVDLNMIQHSSDASFIYVAADKEGKKIAQRRQIT
                     VGKTYDGHAEIVSGIQKGDRVVTSGYQNLIDGQEIKF"
     gene            9097..12522
                     /locus_tag="PLM90_03730"
     CDS             9097..12522
                     /locus_tag="PLM90_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ88509.1"
                     /translation="MKDIFKEFKPSSWAIDNRTTIFVITVILTFMGISTYNALPKELF
                     PDITMPTVYVSTIYPGSSPSDMENLITKELEKDIAAISGVNKITSNSVQDFSSIQVEF
                     ISGTDINDANQKVKDAVDKAKPRLPSDLDPTFGPNVMKVEFSEIPIMQVNVSGDYDLG
                     SLKKYAESLQDKIEELPQISRVDIIGAPEREIQINVDMYKMQAAQLTMYDVYAAISNE
                     NRIISGGTMKVENMRRSITVSGEFDKIEQIQNLTINNANNYPIYLKDVAQIKDDFKEQ
                     ESFARLDNKKVITLNVIKKSGENLIEASDNINTLLEDVKAKDIPGDVNITITGDTSTT
                     TRTQVADLINTIIIGFLLVTIVLMFFMGATDAMFVGLSVPLSMFIAFVVLSLLGMSMN
                     VIVLFGFLLGLGIVVDDAIVVIENTHRIFANGKVPIKTAAKNAAGEVFLPVLAGTMTT
                     LTPFFPLLFWPGMIGSFMKGLPITLIITLTASLIVAYLINPVFAAAFMKPHHDHEKPN
                     RRRHLIWSAILIAVGLLFDASGKMLFGNIVISLVAVFWFYNLVLIHAVRWFQKKGWPA
                     FQHRYASILRWTLNHPGKIFLGILGTLILAIALIVVTKPGVEQFPSGDPNFIYTYVVM
                     PTGTDQQTTDSVTQIVEKRIYEVIGKDNPLVKSVISNVTIGASEDPFDQSAASNKGKV
                     GIAFIDPSERRHQKAEPYIEKIREAVKDIPGADITVGVEQNGPPAGKPINIELTGENF
                     DDLIKTSELVKRYLDSLNFAGIEELKTDLQANKPEIVIDLDRERMNREGISTLTVGGE
                     IRNAVYGWEVSKFKDGNDDFPIIVRYDESQRKNIDQLKDLKITYRDMTTGQIRQIPLS
                     AFATIDYASTYSAIKRIDQKRVVTVSSNLLTGYESQQPVIMNQIKAALEGFDTPEGVT
                     IGYTGASQDMEETFSFLGVAWLISLFLIVIILVAQFNSISKPIIIFAEVLFSIIGVLL
                     GYGLFGMDFSAVMTGVGIVALLGIVVRNGILLVEFTDILIAEGVPLREAVVEAARIRM
                     TPVVLTATATILGMIPLAIGFNIDFVGLFTHGKPDIWLGGENVVFWGPLAWTIVFGLG
                     FATFITLLVVPVMYLLNERFKAWVFPLFGFTYKHKVSDTHGKAKASMELLDLD"
     gene            12655..13878
                     /locus_tag="PLM90_03735"
     CDS             12655..13878
                     /locus_tag="PLM90_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ88510.1"
                     /translation="MRVFVTMVLLIAGTIGFAQSGFTIYNQDNSDLPTNLVNAITIDD
                     HNNTWVGTEGGLVKIDAVGTWTTFTTANSGIPDNQIRSIFIDENDVVWVGTYLDGLAS
                     YDGTDWVAYDPTNSGLPDYHIKSIAKDANDTMWIGTSWGLAKWDGADYWYTYTELNSD
                     ILVPNINALYIDSNDVVYIGTLNGGLNVYDHGVFTIYRTANSTISDNTILSIDEDIYH
                     NKWLATSFGGLSVFTEAGDFLKFTPLTSDISDWSVDAVRLDNNNLGVIGMSATGLEIF
                     NNISWTHYTTANSELPDDFINALAIDSANIIWIGTEEGGLVKFDRSAVAVNTIGQVEV
                     LTYPNPATDNALIIGLPENLSVQIYNNQGMLCGIAEYRNGMHIVNAAKLSAGTYWIYA
                     VDQHTTYTASLQVVH"
     gene            complement(13875..14303)
                     /locus_tag="PLM90_03740"
     CDS             complement(13875..14303)
                     /locus_tag="PLM90_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88511.1"
                     /translation="MKKLLLATTVFAFGLVACDRNEIPDYDVRDEYVGMYDVTDACDA
                     MLNDYTLEVYKGSSYDEIVFGWPGLYEAGMDVTGIVTGMKVVIPIQQFTISTYPNIFY
                     EFSGSASLEDSILTVDYTVLTVQDGLIIDEHNCIATMERN"
     gene            14401..16422
                     /locus_tag="PLM90_03745"
     CDS             14401..16422
                     /locus_tag="PLM90_03745"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HMZ88512.1"
                     /translation="MSQSADLTGQFREQILNGIPSAIPAAKPYQQGINHAPKRKDILT
                     KEEKSLALRNALRYFPKHMHADLAPEFAEELKTYGRIYMYRFRPEYVIKARHYKDYPH
                     NSVQAACIMLMLQNNLDDAVAQHPHELITYGGNGAVFQNWAQYLLAMKYLATMTDEQT
                     LVLYSGHPMGLFPSHKNAPRVVVTNGMMIPNYSKPDDWEKFNALGVTQYGQMTAGSFM
                     YIGPQGIVHGTNITVLNAARKIDTHAQDCAGKVFVTAGLGGMSGAQPKATVIAKGVCV
                     VAEVNPEATTKRHAQGWVDEVFTDLDTLISRMQEARKNKEAVSLAYQGNVVDLWEKLA
                     NDNIQIEIGSDQTSLHNPFAGGYYPAGLSFEESKRMMAEEPELYKQKVYESLRRHVAA
                     INKLTAQGMYFFDYGNAFLLEASRAGADIMLPNGDFKYKSYVQDILGPMCFDYGFGPF
                     RWVCCSNDEKDLRLTDMIAAEVMETIYADAPAEIKLQMSDNINWIKSAMENKMVVGSQ
                     ARILYADAEGRIKIAQAFNKAVREGKLSAPVVLGRDHHDVSGTDSPYRETSNIYDGSR
                     FTADMAIHNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMVLDGSEAADRKLQMMLF
                     WDVNNGIARRSWARNKEAMFAIDREMHRTPELTVTMPQMVDMDLDKFWE"
     gene            16532..18949
                     /locus_tag="PLM90_03750"
     CDS             16532..18949
                     /locus_tag="PLM90_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ88513.1"
                     /translation="MLSRLRFSFLQIGILFVLTSILLPAASQTLSFGLMQPITLSGDT
                     TMVITRDYILQPSMISNVTTPDFLQARLSDNKDTLLLIRNGREMQTLSTLHFSAGNEM
                     FDVLLKKPRKIPVSISFDPNGKKYTQVNIVGDLTNWNPNVLHMQMVNGKWVLPLQLNP
                     GRYQYQIVADGNWFLDPGNKDSIDNGIGGYNSLLNVGGSEGDAPDIYTVWEDSLTNTL
                     DVSGSAEHYVVFWDNHQLTYKEIYSQHEEDFSEGKDIVIAVPEQAKSVKRSQIRIYAY
                     RGNLISNDLLIPLEYGKVITDSKLLDRTDFQKTIMYFMMVDRFVNGDKSNDHPVDDAR
                     VMPQANYQGGDLAGVKQKIDDGYFEKLGVNTLWISPVFQNPMGAYQEFPEPHRWFSGY
                     HGYWPISLSKVDVRFGTNAEFKSLVDDAHGNDMNIFLDYVANHIHAEHPLWTSHPEWF
                     SQLDLPDGRKNLRMWDENRLTTWFEPYMPSFDHSQPAVAEACADSAMYWLTTFGIDGF
                     RHDATKHIETEFWRLLTFKIKTEVAIPQQKPIYQIGETFGSRELIGSYIGSGLLDAQF
                     DFDLYFDARAVFASGDIPFSRLSSSLKESFDYFGWHHVMGNISGNHDMARFISYASGD
                     LKWNENAKEAGWNRHITITDTIGYARLKMLQAFNMTIPGIPIIYYGDEIGIPGGDDPD
                     NRRMMYFDSLNRFEKDVFNTVSKLTQLRSNHMALLFGDFEVLQEQSAGSNDLQFAYLR
                     SYFNDAVIVVFNRSSKQATLSYPLPTRLLNVIPQAQMGTTINRHGYQIDILDIPANSF
                     EIITFDR"
     gene            18977..20305
                     /locus_tag="PLM90_03755"
     CDS             18977..20305
                     /locus_tag="PLM90_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ88514.1"
                     /translation="MTRNFVAAVMLLMSTVALNAQVQAPEWSKDKVIYEVNIRQYTPE
                     GTFNAFTAQLPRLKQMGVGVLWLMPVQPIGELNRKGSLGSYYSISNYVTTNPEFGTLK
                     DFKKLVDEAHKLGMYVILDWVGNHTAWDHPWITDHPEYYTTDKNGKIQPPVADWTDVA
                     DLNYDNMQLRKAMIHDMAFWVKETNIDGFRCDVAMMIPDAFWQYAFNEMREIKPELFM
                     LCEAEGPQFVKDGFNMVYGWERHHAMNQIAKGKGTVDHLDSLVQKDIASYAPISYYMN
                     FTSNHDENSWNGTEFERMGDGARAFAVLSATLPGMLLIYSGQEVGLDRRLKFFDKDSI
                     NWVDDKQFTPFYTKLINIKTQNQALWNGNYGGSYRRVACENSGTYIFAREKDGNKVLV
                     VANLTAEISNIVFANEAGKYIDAMSGKKEKVKATEKMTLKPWEVKVLVKK"
     gene            complement(20448..20831)
                     /locus_tag="PLM90_03760"
     CDS             complement(20448..20831)
                     /locus_tag="PLM90_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMZ88515.1"
                     /translation="MKKLIALTISLLMLVSMQAQQRNDIQVGGNQTQMKDGCGAPVGV
                     FVDEITLISATIHWNTNGAAYYKVQFYNVNNPREKGTLMTNLGSVMLDNLTPCATYRF
                     IITAKCADGLSKTQAMSFSTKGCSN"
     gene            complement(20992..22461)
                     /gene="guaB"
                     /locus_tag="PLM90_03765"
     CDS             complement(20992..22461)
                     /gene="guaB"
                     /locus_tag="PLM90_03765"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMZ88516.1"
                     /translation="MEHLSTKFLGESLTFDDLLVVPAYSEVLPREVDISTRLTRNLRI
                     NVPIVSAAMDTVTESQMAIAMAREGGIGIIHKNMTIEKQADQVRRVKRSDSGLIIDPV
                     TLSKTATLRDALQMMREFKIGGIPVVDDNQKLVGILTNRDLRFETELSRPVSEVMTKE
                     NLITAPEGTTLAQAEDILRNYRIEKLPVVDASGTLKGLITYKDIKKLLNHPRACKDSF
                     GRLLVGAGVGVTHDTLQRVEALRSVGVDLVIIDTAHGHSRGVLDMVRLVKKTFPDLEI
                     VAGNVATGSGAKALADAGVDGVKVGVGPGSICTTRVVAGVGVAQVSAIYDAAQALKGT
                     GVPVIGDGGIRYTGDIVKAIVAGADTIMAGSLLAGTEESPGETIIYEGRKFKSYRGMG
                     SLEAMEKGSKDRYFQDVEDDLKKLVPEGIVGRVPFKGTVEEVMVQYIGGLRAGMGYAG
                     ARTIEDLKTAQFVKITASGIRESHPHDVVITKEAPNYSR"
     gene            22651..23529
                     /locus_tag="PLM90_03770"
     CDS             22651..23529
                     /locus_tag="PLM90_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88517.1"
                     /translation="MKTLAFFLAFSYLSSRLFSQVYIIPNAELQPSWTFPLCFEDASG
                     ARDTIYMAYDIDAIDYSIPSADTIFGEAFLPIDTSEFRAYWEYIYDGKAVKTLVMKNF
                     IPAQINFNKAQLPVKFTWDAQLFYSENLPFPNNYPLPNAWGLVHCSSWKDFEGCQLDE
                     YSGIYLTNEPDFVYTDVATDSILYQSEYYYFFENGLDFRILQYGTGYNWATIVENESQ
                     SFQIYPNPSDDFIHILTKENTKATLIISDMQGKTIKQFVDVSNTTIPVYDLLPGMYMV
                     NYSSYESAYSSKFIKK"
     gene            23720..24649
                     /locus_tag="PLM90_03775"
     CDS             23720..24649
                     /locus_tag="PLM90_03775"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMZ88518.1"
                     /translation="MIPRRNGILLVETFTILILNGVLATYMHSHLPTTLFRCCYAPLL
                     LFQGLWYYRIYIVGHEAAHKKLIPAHLLLNEIAGSLVLLPLLTPLNIYRKIHYFHHGF
                     NRKDPHTSALDTFVVKGKYRTLKTAYYYVVWYLSVFCGGFFLHSLVSVILFLFCPPAL
                     ARKISPAFNGWNYKDQLKAILLFLAGILIHLWVFTFFGKTAYLLSLGLPMLVFAWLLS
                     MLVYVFHYDTTMGEHVRYNVRSVNRIPVLSWILMNFNEHATHHQYPNIPWYSLPENRS
                     ELPEEFGNKNQSTRNLFVAVIRQLKGPHVIYQP"
     gene            24630..25271
                     /locus_tag="PLM90_03780"
     CDS             24630..25271
                     /locus_tag="PLM90_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88519.1"
                     /translation="MSYINLRYGKQEDVDAMMYIKHQLPLKTADGNPVKGGFLLGTTR
                     EQYLSFILNDHCLVAEDHGNVVGFGIMLHNASLRNSDIWQRRHQAAWEVDINDFEDLP
                     ICYFEQLAFLPGYNRAVLKLAYQLVMDAFRQGHTHLFATTVHKPVLNIAAVPFIKKAD
                     GGLVGRIDEYYPEVGNILSDIYLIDRIKFYDAVPKLAIYPWLIKSLAGDSKLV"
     gene            25395..25859
                     /locus_tag="PLM90_03785"
     CDS             25395..25859
                     /locus_tag="PLM90_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88520.1"
                     /translation="MKSLLIIGVLTLSCMSKLHAQKINPDYDSTLAKQLNADDYGMKS
                     YILVILKTGSNNVQDKQLRDSLFAGHFANMSAMVEAGKLVVAGPIDKNENNYRGIFIL
                     NVTSFEDAQLLLQNDPTIHEKVLEAELYEWYGSAALPVYLDTHDKIQKFSIE"
     gene            26089..26625
                     /locus_tag="PLM90_03790"
     CDS             26089..26625
                     /locus_tag="PLM90_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88521.1"
                     /translation="MRNTQAQVLQADTLPQSEYLNFYISASSEDFYIKFTQVPNKLFG
                     ESLKVRINNESGVFAIPIINEENYSIDLPGRSSPYRSDTLYFYVEMYDQVYHSIAVVI
                     FPGFMRNISFGEGCYELDCTPVQDPYMPSVVVPFLIDPDAAYKIPGRNKIITGKMALS
                     RYTPCDLEKFEQVFFQDN"
BASE COUNT         8052 a         5213 c         5734 g         7998 t
ORIGIN      
        1 tcggcagtat tatgatagaa gaaaatgttg tcagtgcagc aggcgctccg catcgattca
       61 cttataaatc tattcaagaa gcgattcgtg aagccggatt tgaaccgcaa ttgcgcacac
      121 agcaatatga agatcgcgag attcctgcca atatccagga acaggtgatc aactattaat
      181 aattgtgctt atcgcgttgt gtgtattttg tagtaatata tatatattac catctaaatc
      241 tatttgttta tgaccaacga ttatatttca agcgttaaaa atcaattcga gtactataaa
      301 atgcttggcg aaaaaacctt tgatcaggta acggatgaac agctcttttg gaagtttaac
      361 gaagaaagta attcgatcgc tactatcgtg aaacatttga gcggcaacat gttgtctcgt
      421 tggacagatt tcttcacctc ggatggagaa aaagaatgga gaaagcgtga tgaggaattt
      481 gagaacgata ttcaaaacag ggaggacatg ttgaaagtat ggaatgaggg ttggaactgt
      541 ttgtatcatg taattgatca tttaactgct gatgatctgg agaaaattat ctacatcaga
      601 aatcaaggtc attcagttgt agaagctatt aacaggcaac tggcacatta tccctatcac
      661 gtaggacaaa ttgtttttat aggtaagatg gtaatggatg aaaaatggaa atcgttgtcg
      721 atacccaaag gaaattcaaa agccttcaac aaagaaaagt ttgataaacc aaaagaaagg
      781 cgtcatttta cggatgagta tttgaataag gatgatggga agtgattatt ggtttacata
      841 cttaatgggt gtctgaatga taatcattaa caagaatcta aataacttaa acttggagtg
      901 tcaattgaag taagattgtg agttgaaatg aattcatcct ttggatatat ttatgtattt
      961 ataatagttt gctcaatcgg gtgcacaagg acttccaatg aaacaaaaag taagacatta
     1021 gttaatagca atatttctga agatgtagat tcgtcttccg gtagtgattc gcttgatgtc
     1081 atagaattta ttaaagtctt tgcattagat cgcgctgata catttattgt tggcgatgta
     1141 aatggcgaca gaaaagttga taaggccatc attgaaccgg ctacctttat ttatcgcgat
     1201 ggtaaggtgg atagtcagta tgtgaacata tcctttacaa ccgatattcc tgttatcaga
     1261 cattataatg gttttcatgg attgattgca aatgtcggcg accttgatgg taacaagacg
     1321 gatgagttga tttattatcc tgattggttt atcggtaata tggcgagtat ttacatatat
     1381 ggattgcgag atggtgaatg gaaattgttt gccgacggat cccagagacg agatattatc
     1441 tttgaaacca aggatccttt caagtatttg cagagtaggg ttgaaaaaat tgacaatagt
     1501 tcttttaagt tgactgaaat tactatgaat gaaaatgctg agtttattga ttcaacttca
     1561 atagtgttta ttcattaaat ttattttacg aatattaata ccatgaaact atcagtaaaa
     1621 ctgtttctcc atattttttt attctgctct tcattcttaa tggcagcaca agactccaca
     1681 tttagtgtaa taacatttaa ccttcgatac gataatcccg ccgatgcgga aaataattgg
     1741 cattatagaa aacaggaaat agccgatttt attcaacttc gttcacctga gatttttggc
     1801 atacaggaag ggcttatcca tcagttagct tttctggata gtgcacttac gcgatatcaa
     1861 tatgtcggaa aaggtcgtga tgacggtgca caaaaaggtg agtttagcgc catttttttc
     1921 gacacatcta aggttacatt aatttatgat tcaacctttt ggctttcact tacacctagt
     1981 gaaccttctg ttggatggga tgcagcgctt ccgcgtattt gcacctttgc gcgttttgtg
     2041 actaaaaatt ccggcgatac cttgaatgta tggaatatgc acctcgatca catgggcatt
     2101 accgcacggt ataaatcaat agccttgctc gtggtaatga taaatgattt tgataaggca
     2161 ttgtggcttc aacctatgat cattatgggc gatttaaata gcacaccact cgatgatcct
     2221 gcacatacca tgctcaaatt tgccgaggat acaagaacag cgacgctcac ccctccactt
     2281 ggtcctgatg ctacatttaa tggatttgca ccgcttaata ctgaaagtaa gcgaatagac
     2341 tatatttttg tgcgaaatct tacaaccatt gctcaagaac atatcgacac aaaacgatcg
     2401 aatggattgt ggttgtcgga ccatttacct gttatggcta cactttcttt caaatagtta
     2461 agcatgaagc tacattctat tcgcctctcg ttgcttattt gtctgatcac cttcggaatt
     2521 aactacatgc aagcttgcag catgagtaag atcacattta atggagttac ttatgtaggt
     2581 aataatgaag acagctggca taccgatcca cgcatctggt ttgaaccggg agccggaagc
     2641 aaacgcggtg cctgttatgt cggttacgcc gataaatggc ctcagggtgg tatgaacatc
     2701 gacggtttgg tgtatgatgc atttactatg tacgatggaa atgttcaacc caatggaaaa
     2761 ccaattgcag aaaacctgcc gattttcctg cgcgatataa tgcagtcctg cggaaccgtt
     2821 gatgaagtat ataagtacgc tatcaacttc gacagacata atttcgaccg agcaatgatg
     2881 ttgtttgtcg ataccaccgg aaaatatctg gtgatggaag cagataccat gattattggt
     2941 aacgatagca catttttgtt ggcgaatttt tctttttcta aaactaaaga cctttctaca
     3001 gtatcgttgg gcagatatca aagaggtcgc acatttctgg aaaaacatga aataggagga
     3061 cttgattttt gcagagcatt gatggatacg atgtcggaat gtagggaaag aaaaggtgat
     3121 ggcaccctgt atacttcatt gtatgatacc aaacgccgat taatttcatt gtgcttttat
     3181 cacaattatg atcgcgtgca tattctctca ttgaatgatg aattggcaaa aggtgaacac
     3241 tggattgcca tggaaactct attccccaag aatgaggaat ttgaaacatt aaaatcatac
     3301 atcacgcccg caaatcatat caccctattg ccaatcatgc tcattgttgt tattttattg
     3361 ctcggcatgg cagcaatttt atcgacacaa tggttcagac aattacatct tgctgaaaaa
     3421 aatcttcagt atttgcgcat tagtattgcc atttttaatg cactgttggc aatatatatt
     3481 tgggtcatca tgaatgaacc tgctgtgtat tatttccccg ctccatattt cgaagccggt
     3541 cgcggatgga tcaatttgct ttcttacatg cctgttttaa cattgatagt gatggcagct
     3601 accggatggc tggtttttcg caaacaagtg gtcactacaa ccggcgccag ggttgggttt
     3661 tatgtcaatg ctgttgtttg gttactatgt gcaatagcat tcgcttattg gggactcatt
     3721 gctattattt aacaaataac tgtcctcttt ttgcatcatc aggcattatt gttgcggaat
     3781 acttctgtat agcagtttgc cccggtggca tttgcttcca taattttaca tcttaaacac
     3841 cttacatgaa aaaattaatt attgcgtgca tggtattatg caccataacg gtaaaagcac
     3901 aatcacttga aagtggaacg cttagttttc aagtgggcta tgatgctatg atacacggta
     3961 cagcgagtga aacaacattt tttggtgttg tagtagacag agataccagc ggcgccgtca
     4021 ccagtatgtt taatgcagat gcgcaatata gttttgcccc ttggttttct gccggtgttg
     4081 catttaatat aggatcttat ctggaagata cggcagatca atctaatgat ggcaatcacg
     4141 tatccattat tgcatttgat gcgagattgt atcctgtcaa taacgataaa tttaattggt
     4201 atctgggtgg ttcggcagga ttgacaaatc ttgaaatcaa taaaaaagat gcgctcactc
     4261 caggtgtaaa ttatcaatat aaatataaaa gcccggccct tggattattt accggcttca
     4321 attggtattt cttcgatgtt gccggtgtgt ttctgcaatt tgaatacagc cagcaaaatt
     4381 tcgaccttaa atcgtttagt atcgatggcg cagaacaaga tctctcgcaa actacgcagt
     4441 cgctgataac taaaggtggc ggcatccgtc ttggtgttaa ctttaaattg aattaggaca
     4501 ttttacttta gagggagagt agatagcctt atgttaaact actcttcctc tatatacttt
     4561 cttcctttca tcacaaaata acgctgtaca tttgtcgcat gcgttttttg tcggccgatg
     4621 tcatttatcc ttcctacgga aatccacttc gtgagcatgt gctcattgtg cagcacgatg
     4681 gtcgtataga tggtattgtt gctaaagctg atatcgatcc actcaagatc gagcattttg
     4741 atggtgcact ttgtcccgga tttgttaacg cgcattgtca cctcgagcta tcccatttgt
     4801 tgggaaaatt tccaatgaaa acgggtttgc ctgcatttct gatggaagtg accaggcaac
     4861 gtgaaactgc aatggaggat attatttcag ccatgcaaga agctgatagt agtatgtggc
     4921 aaaatggtat cgtagctgtt ggcgatatca gcaatgctcc gcatagttta gacattaaag
     4981 ctgatagccg catatattat cacacctttg ttgaattgct ggcacttgat ccgcagaaag
     5041 caacaacggc tattgaacat ggaaatgcaa ttgttgcttt ggcgaaggaa agaaatttaa
     5101 gtgcctccct tgcacctcat gcgccctata ctatcagcac cttgttggtg gaaggcatca
     5161 cccgtgcctg tcgtgatatg ggtatgccaa caagcattca catgctagag agcaatgacg
     5221 agaatgagtt ttatgttcag gcaaccggtg gatacagaaa attatatcgc gaactgggaa
     5281 ttccgatcac acatttcgag ccaacaggaa aaacttcatt ggagtcaata ctgcctttta
     5341 tggataagga cgtgaaaacg ctcctggtgc ataatactat tgctaccgtt tgggatgccg
     5401 attgggccga aaatctgcat gccaatttgt attggtgttt ttgtcccaat gcgaatttgt
     5461 tcatcgaaga tcgcctcccc gatatcccgg aactcatgca acatatcaag catattgcca
     5521 tcggcaccga cagccttgcc tccaaccatg gcttgtccat cctcgacgaa ttgaagacca
     5581 ttgcccatac ttttccttcc cttaccacgg aggaactgct ccaatgggct accgatggcg
     5641 gcgcacaatt tcttggctta tctgacaggt ttgggagcct caaacccggt ctttcccccg
     5701 gcattctgca cctgatcaca agtgaaattc agccagaaac ccttgtaaat aggctgtttt
     5761 aagctccatc cccgcatttt acccattcat ctcaattttt ggcggtttcg tcgaaacgcc
     5821 tggcgttttc ccggttttcg aggtcaattt tgcctccgaa aagggctgaa atgttaaaat
     5881 caaataaaaa ctatggaaac tttcaaacaa ttcttggttt cctcggtttc ctgtcagacc
     5941 tttgcgccgc caatgaacga agaagtaaaa aaaatactca atcgctgtga acgtctcttc
     6001 atgaagtacg gcatcaaaag cgtgaccatg gatgacgtgg cgcgcgaact tggtgtaagt
     6061 aagaagaccc tttaccagta tttcgagaac aaggaacaac tcatccaaac ggtgactgag
     6121 aatcacttta aatgccagaa tgaaatggtg caacaagtga ttcatcactc taaaactgcc
     6181 atcgacgaaa tgtattcgat tgcagcctgg atgaatgcga tgagtaaaaa cctcaatcct
     6241 gccttgttat atgatttgag aaaatatcat ccggaaagct ggcaggtatt tatcgatcac
     6301 cgaaataatg aagtgctcaa cagcatccga cataatctgg agaggggcat aaatgaaggt
     6361 ttatatcgcg cagatctcga tattgaagtg atagctcgta tttatattac acgcgttgaa
     6421 atgtttatcg ataatgaaat tttcccttta gataaatttt cacctgaacg aacatttaac
     6481 gtgttcattg attatcactt acgcggaata gcaacgccaa aaggcataaa atatttagaa
     6541 caactcaaat cacaaaacca tggcatataa attcaaagtg ttatgcatgg ccgtactcct
     6601 ccttccggca atattacaag cgcaaactat gcgtttttcg ttgcaggaag caatcgacta
     6661 tggcctgaag aacaatccgc agattaaatc tgcagagctg gatgtgcaga atgcagaaat
     6721 caccgttgac aatcgtattg cgtcaggatt acctcaggta gatgcgaaac tcgattacca
     6781 aaatttcttt gtactcccca catctcttat cccgggcgaa ttttttggcg gtgaacccgg
     6841 aacatttata cctgtgcaat tcggaacaga acagaatatg actgcgggta tctccgcatc
     6901 gcaattatta tttaacggtg catggcttgt tggcgtaagc gcagcaaaac aatatgcggc
     6961 attggtgcaa acacaatcaa aaattgcact ctctgatgtc cataaaaatg ttgaatctgc
     7021 ttacaactcg gtcctcgttg cgcaggaatt cgaaaagctg cttcaaaaaa atatcggcaa
     7081 cctcgataag gtattattcg aagttaccga attgaacaag cagggatttg ttgaagggat
     7141 tgatgtagat cgtcttacta tcagcaaaac cacattgcaa ggtcaactcg acaatgcaaa
     7201 acgtcagacc gcactcgccg cacagtattt gaaattcagc atgggacttg atatgtccac
     7261 tgcaattgaa ctcacagata cattggcgcc aattaaggaa gtgaatatgg ccggaagcaa
     7321 ttatttggct cgccctgagt tcgctgttat ggaaagtctg gtgcaattaa atgcattaaa
     7381 tataaaagtg aataaagcga tgtatttacc ctcattggtg ttgtatggtt catattcaca
     7441 aaatgcacaa cgcaatgaat tcgacttttt aagtggcgat ggtcagtggt ttgaaaccgg
     7501 tcttgtagga ctcacgcttt cagttcctgt tttttcgggt atgcagcgcc gctcaaatgt
     7561 gcaaatggca caggtggcat atgaaaaagc aaaactcgat cagtttactg ctacacaagg
     7621 tattttgctt gaagtagaaa aggcaaaaac agattatatg aatgccgcaa atgaccttga
     7681 agtgcagaag aaaaacatcg cactggcaca aaaaatatat gatacggctt tgatcaaata
     7741 tcgcgaaggt gttggatcaa gcctcgaatt aagtaccgca gaaagcacac tgtaccaagc
     7801 acaaggaagc tatatcggtg cactattcaa tgtattgaat acacaaacat ctcttaacaa
     7861 agcattaggc aactattaaa acccaatatt tatgaaaaag aacttcatat atctcgcaat
     7921 ggcactcttt attgcggcct gctcaccaaa agagtcgggc gacaaaaaag ctgagctgga
     7981 agcattgaaa aaagaacgta cagcactgga taaacaaatt aaagagctgg aggcagaaat
     8041 cgcaaaaacg gataccacaa aatcaaaaga tcctgaactg gttgtagtaa cggaaattca
     8101 accgcaagaa ttcacgcact atatcgaaat tcaaggtaag atcgatgctg atgaaaacgt
     8161 tttagttact cctgaaatgc ccggtgttat taaagcagtt tatgtagtag caggacaaag
     8221 cgtttctaaa ggacaattac tggccgaatt agataatgat gccatgcgca aaaatattga
     8281 aacactcgaa tcgcaactcg gatttgctaa ggagatgtac aataaacaaa aggcattatg
     8341 ggatcaaaaa atcggaagtg aagtgcaata cctttcagca aaaaataatg ttgaaacatt
     8401 ggaaaaaagt attgccgctg ctagagaaca attaaatatg agcaagctta catcaccaat
     8461 cagcggttta gtagataatg ttgatttaaa agcaggtcag atcggttctc ccggatttca
     8521 gggtattcgc gtggtgaata tgagcagcct gaaggctaaa ggtgaagttt ctgaatcaca
     8581 ttcaaatgtg gtgtcaacag gagataatgc taaaattatc atcaccgacc tcaacaaaga
     8641 gatcgatacg aaagtatcat ttgcagcacg cgttattaat ccgcaaagcc gcactttcag
     8701 cgttgaagca cgcattacag gtgagtctga tcttaagccg aatatgatag cagttattaa
     8761 gattgccgac tatgaagcga aggaagcaat ggttgtggat ttaaatatga ttcagcattc
     8821 aagcgatgcg agcttcatat acgtagctgc cgataaagaa ggaaagaaaa ttgcacagcg
     8881 ccgtcagatc acagttggaa aaacatacga tggccatgct gaaattgttt ccggaatcca
     8941 aaaaggagat cgcgtggtaa cctcaggata ccaaaacctg atcgacggac aagaaattaa
     9001 attttaacat agagagaaca ggaatcgatg atgtaattcg aaatgaatga tatgcatcaa
     9061 taaataaaat tccaattctc aaaaccatat acttctatga aagatatatt caaggaattt
     9121 aaaccttcga gctgggcgat cgataaccga accaccatat ttgtgattac ggtgatcctc
     9181 acgttcatgg gtatctctac ctataacgca ttgccgaagg aactttttcc ggatatcact
     9241 atgccgacag tttacgtgtc gacaatctat ccgggatctt ctccatccga tatggagaat
     9301 ctgatcacca aagagcttga aaaagatatt gctgcaatta gtggtgtgaa taaaatcacc
     9361 agtaattccg tacaggactt ttcaagtatt caggttgaat ttatttcagg aaccgatatc
     9421 aacgacgcca atcaaaaagt aaaagacgcc gttgataaag caaagccgcg tttaccttct
     9481 gacctggatc caactttcgg tccaaacgtt atgaaagtgg aattcagcga aattcccatc
     9541 atgcaggtaa acgtgagtgg tgattatgat cttggttcat tgaaaaaata tgccgaatca
     9601 ctgcaagata aaattgaaga gttaccacag atatctcgcg tagatatcat cggtgctcct
     9661 gaacgtgaaa tacagatcaa tgtcgacatg tacaaaatgc aagccgcaca attgaccatg
     9721 tatgatgtgt atgccgctat cagcaacgaa aaccgcatta tctcaggcgg taccatgaaa
     9781 gtggaaaaca tgcgtcgcag tattacagtg agcggtgaat ttgataaaat tgaacagatt
     9841 caaaacctca ccatcaacaa tgcaaataat tatcctatct atctgaaaga tgtcgctcaa
     9901 attaaagacg acttcaaaga acaggaaagc tttgcgcgtt tggataataa aaaagttatc
     9961 accctcaacg taattaaaaa gagtggtgag aacctgatcg aagcatcaga taatatcaat
    10021 acattattag aagatgtaaa agcaaaagat attcccggcg atgtaaatat taccatcacc
    10081 ggtgatacct ctactacaac aagaacacag gttgccgacc ttatcaatac cattattatc
    10141 ggcttcctgt tggtaaccat cgtgttgatg ttcttcatgg gcgcaaccga tgcgatgttt
    10201 gttggtttat ctgttccgtt gtcaatgttc atagcatttg tggtgctgag cttactcgga
    10261 atgtcaatga acgtaatcgt attattcggc ttcctgcttg ggttgggaat tgtggtggat
    10321 gacgccattg tggtgataga aaatacgcac cggatattcg ccaatggtaa agtacctatc
    10381 aaaacagcag cgaaaaatgc cgcaggtgaa gtattccttc cggtattggc aggtaccatg
    10441 acaaccttaa ctccattctt cccattgttg ttctggccgg gtatgatcgg ttcatttatg
    10501 aaaggtttgc caatcacatt gatcattaca cttacagctt cattgatcgt agcgtatttg
    10561 atcaaccctg tgtttgccgc agcattcatg aaaccgcatc atgatcatga aaaaccaaat
    10621 cgccgcagac atttaatttg gagtgccata ttaatagcag ttggtttgct cttcgatgct
    10681 tcaggtaaaa tgttgttcgg aaatattgtc atttcattgg ttgctgtttt ctggttctat
    10741 aatcttgtat tgatacatgc tgtgcgttgg ttccagaaaa aaggatggcc tgcattccaa
    10801 catcgctatg caagtatttt gcgttggaca ctaaatcatc cgggaaaaat attccttgga
    10861 atattgggaa cattaattct tgcaattgca ctaattgttg ttacaaaacc gggtgttgag
    10921 caattccctt caggcgatcc aaactttatt tatacatatg tggtaatgcc aacaggtact
    10981 gatcagcaaa ctactgactc agttacacag attgttgaga aaagaattta tgaagtgatt
    11041 ggtaaagaca atccattagt gaaatctgtg atcagcaacg ttaccattgg tgcaagtgaa
    11101 gatccattcg atcagagtgc tgcatcaaat aaaggaaaag ttggtattgc gtttattgat
    11161 ccttcagaac gcagacatca aaaagccgaa ccatatatcg aaaaaatccg cgaagctgta
    11221 aaagatattc cgggtgccga tattaccgtt ggtgttgaac agaatggtcc tccggcaggt
    11281 aaaccaatta acattgaact taccggcgag aatttcgatg atctgataaa aacgagtgaa
    11341 ttagtaaaac gctatctcga tagtttgaac tttgcaggta ttgaggaatt gaaaacagac
    11401 cttcaggcaa ataaacctga aattgttatc gacctcgatc gcgaacgcat gaacagagaa
    11461 ggtatttcta cactaaccgt aggtggtgaa attagaaatg ccgtttatgg atgggaagtt
    11521 tcgaaattta aagatggtaa cgacgatttt ccaatcatcg tgcgctatga cgaatcgcaa
    11581 cgtaagaata tcgatcagtt gaaagatctt aagatcactt atcgcgatat gactaccggg
    11641 cagatacgcc aaattccatt gagtgcattt gctaccatag attatgcatc tacttattct
    11701 gccatcaaac gcattgatca gaaacgtgtg gtaactgtct cttcaaatct tttaacagga
    11761 tatgaaagtc agcaaccggt tatcatgaat caaattaaag cagcgttgga aggttttgat
    11821 actccggaag gtgtaacaat cggctatacc ggtgccagcc aggatatgga agaaactttc
    11881 agcttcctcg gagtagcatg gttgatttca ttattcctta tcgtaattat tcttgttgcg
    11941 caattcaact ccatttctaa acctatcatc atcttcgctg aggtattatt tagtatcatc
    12001 ggtgtgcttt tgggatatgg tttatttgga atggacttct ctgccgtaat gaccggtgtg
    12061 ggaattgtag ccttgctcgg aatagttgtt cgtaatggta tcctgctggt tgaatttacg
    12121 gatatcttga tcgctgaagg tgtgccactg cgtgaggctg ttgttgaagc agcgcgtatt
    12181 cgtatgacac cggtagtgct cactgctact gccaccatat tgggtatgat tccattggcg
    12241 ataggcttta atatcgattt cgtgggatta tttactcatg gtaaacctga tatctggctc
    12301 ggtggtgaga acgttgtgtt ctggggacct ttagcttgga ctatcgtatt tggtctcggc
    12361 tttgccacct tcattacatt attggttgtt cctgtaatgt acttattgaa tgagcgcttt
    12421 aaagcatggg ttttcccatt gtttggattt acttacaaac acaaagtttc cgatacacat
    12481 ggtaaggcta aggcttcaat ggagctgttg gatctggatt aatcggaaat gctaatatat
    12541 aaaaggctgt catgttaagt gacagccttt tttgttgtgt taaaatgagt ttcgtttgac
    12601 taaagtcacc atctagaaat aagaatatct caaataagcc tctaattttg taacatgaga
    12661 gttttcgtca ccatggtttt gttgattgca ggaacaatcg gctttgcgca atccggattc
    12721 acaatttata atcaggataa ttcagatctg ccaacaaacc tggtaaatgc cattaccatt
    12781 gatgatcaca ataatacctg ggtgggtact gaaggcggct tggtgaaaat tgatgcagtt
    12841 ggcacatgga caacatttac tacagccaac agcggtatac ctgataatca gataagatca
    12901 atatttattg atgaaaatga tgtggtatgg gttggtactt atctggatgg attagcgtca
    12961 tatgacggta ctgattgggt agcttatgac cctaccaatt caggcctacc ggattatcat
    13021 attaagtcaa ttgcaaaaga tgcaaatgat actatgtgga tcggaacttc atggggactt
    13081 gcaaaatggg atggcgcgga ttattggtat acctacactg aattaaattc agatatactt
    13141 gtgccgaata tcaatgccct atacatcgat tcaaatgatg tggtttatat cggcacatta
    13201 aatggcggtt taaatgtgta tgatcacggc gtgtttacaa tttatcgcac ggcgaattca
    13261 actattagcg acaataccat tttgagtatt gatgaggata tttatcataa taaatggctg
    13321 gcaacatctt ttgggggatt gagtgtattc acggaagcag gcgattttct caaattcaca
    13381 ccacttacca gtgatatcag cgattggagt gtggatgcag tgcgtctgga taataataac
    13441 ctcggtgtaa taggtatgtc tgctaccggc ttggagatat tcaataatat cagctggaca
    13501 cactatacca ctgcaaacag cgaattacct gatgatttta taaatgcctt agctattgat
    13561 tcggcaaata ttatatggat tggaactgaa gaaggtggat tggttaaatt cgatcgcagt
    13621 gctgtagccg taaatacaat aggacaggta gaggtgctca catatccaaa tcctgccaca
    13681 gacaatgccc tgattattgg attacctgaa aacctttcgg ttcagattta caataaccag
    13741 ggcatgcttt gcggtattgc tgaatatcgc aatggaatgc atattgtcaa tgcagcaaaa
    13801 ttatctgcgg gtacttattg gatatatgct gtcgatcaac atactactta tacagcatct
    13861 ctgcaggtgg tgcattaatt gcgctccata gttgctatac agttatgctc atcaataatg
    13921 agtccatcct gaacagtcaa tacagtatag tcgacagtca gaatactgtc ttccaatgaa
    13981 gcactaccac tgaactcata aaatatattc gggtaggttg aaatagtaaa ttgttgaatg
    14041 ggaattacca ctttcattcc ggttactatt ccggtaacat ccattcctgc ttcatacaat
    14101 ccgggccaac cgaaaacaat ttcatcgtag gaagagccct tatatacctc caatgtataa
    14161 tcgtttaaca tggcatcgca ggcgtcggta acatcataca taccaacata ttcatctctt
    14221 acatcgtaat cgggtatctc gtttctgtcg caggctacca atccgaatgc aaatactgtt
    14281 gtagcgagta ataacttttt cataagcggt tgatttacaa ctacccttaa aaccaacatc
    14341 gtgccaagtg tcggtttttg ggttggcaat tggtaatttt gcccttacaa gtcagcattc
    14401 atgagccaat cagcagatct tacaggccag ttcagggaac agattttgaa tggaattcct
    14461 tccgcaatac cggccgcaaa gccttatcag cagggcatta accatgcacc caagcgcaag
    14521 gatatcctga ccaaggagga gaaatcgctc gccctgcgca acgcacttcg atattttccc
    14581 aaacatatgc atgccgacct tgccccggag tttgccgagg agctgaagac ctacggacgc
    14641 atctatatgt atcgcttcag gccggagtat gtgatcaagg caagacatta taaagactat
    14701 ccgcataata gtgttcaggc tgcatgcatc atgctcatgt tgcaaaataa tctcgatgat
    14761 gcagtagcgc aacatccgca cgaattgatt acctacggtg gaaatggggc agtatttcaa
    14821 aattgggcgc aatatctgct tgccatgaaa tacctcgcca ccatgactga tgaacaaaca
    14881 ttggtgttat atagcggtca cccgatggga ttgtttcctt cacataaaaa tgcaccgcgc
    14941 gttgtggtta caaatggtat gatgatacca aattattcaa aaccggatga ttgggaaaaa
    15001 tttaatgcac tcggtgtaac acaatatggt cagatgacag caggttcatt tatgtatatc
    15061 ggacctcagg gaattgtgca tggtactaat atcaccgtgt tgaatgcggc aagaaaaatc
    15121 gatactcatg cgcaggattg tgcaggaaaa gtttttgtta ccgcaggatt gggaggaatg
    15181 agcggcgcac aaccaaaggc aactgtaatt gcaaaaggag tgtgtgttgt tgcagaagta
    15241 aaccctgaag caacaacaaa acgacatgca caaggttggg tggatgaagt atttactgat
    15301 ctggatacac ttatttccag aatgcaggag gcgagaaaaa ataaggaagc agtttcgctt
    15361 gcttatcaag gaaatgttgt ggatctctgg gagaaacttg ccaatgataa tattcaaatc
    15421 gaaattggca gcgatcagac ttcattgcat aatccatttg ccggcggtta ttatccggcg
    15481 ggtttatctt ttgaggaaag caaacgcatg atggcggaag agcctgagtt gtataaacaa
    15541 aaggtatatg aaagtttacg tcgccatgtt gccgctataa ataaattaac tgctcaaggc
    15601 atgtattttt ttgattatgg aaatgccttt ttattggaag catcgagagc aggtgccgac
    15661 atcatgttgc ctaacggcga tttcaagtat aaaagttatg tgcaggatat tctggggcca
    15721 atgtgttttg attatggctt tggtcctttc agatgggtgt gttgcagcaa cgacgaaaaa
    15781 gatttgcgat tgaccgacat gatagcggca gaggtgatgg aaaccatata tgccgatgcg
    15841 cctgcagaaa ttaaattgca gatgagcgat aatatcaatt ggataaaatc tgcaatggaa
    15901 aataaaatgg ttgtcggcag ccaggctcgc attttatatg ctgatgctga aggaagaata
    15961 aaaattgcac aggcatttaa taaagctgtt cgcgaaggaa aattatcggc acctgttgtt
    16021 cttggtcgcg atcatcacga tgtaagcggc actgattcgc catacagaga aacgagtaat
    16081 atttatgatg gcagcaggtt tactgcggat atggctattc ataatgtcat cggcgatagc
    16141 tttcgcggcg caacctgggt gagtattcac aatggtggcg gtgttggttg gggtgaagta
    16201 ataaacggtg gctttggaat ggtgcttgat ggaagcgaag cagctgatcg caagttgcaa
    16261 atgatgttgt tttgggatgt aaataatggc atcgccagaa gaagctgggc gcgcaacaaa
    16321 gaagcaatgt ttgccatcga ccgtgaaatg cacagaacac cggaattaac agttaccatg
    16381 ccgcagatgg ttgatatgga ccttgataaa ttttgggagt aatatcaatc tggcctgtag
    16441 gctgttttca tcaattgttt gattgcattt tgtttgtgat cagccctaac tcatggattt
    16501 tagcactaat tttgtatgat atcatatcat catgctcagt cgtttgaggt tttcattttt
    16561 acagataggt atactttttg tattaacaag catattgttg cccgcagcat cacaaacact
    16621 gagttttggt ttgatgcagc cgattacact ttcaggtgat acaacaatgg tcatcacgcg
    16681 tgattatatt cttcagccgt cgatgataag taatgttaca actcctgatt ttttgcaggc
    16741 acgattatct gataacaaag acacattgtt gttaattcgc aacggaagag aaatgcaaac
    16801 actcagtacc ttgcatttct ctgccggtaa tgagatgttt gatgtcttgt taaaaaagcc
    16861 gagaaaaatt ccggtatcga tttccttcga ccccaatggt aaaaaatata cgcaggtaaa
    16921 tattgtgggt gatctgacaa attggaatcc gaatgttttg cacatgcaaa tggtaaacgg
    16981 gaaatgggta ttaccgctgc aactaaatcc gggaagatat caatatcaga ttgttgctga
    17041 tggcaattgg tttttagatc ccggaaataa ggattccatt gataatggaa ttggcggata
    17101 taattcattg cttaatgttg gcggcagcga aggtgatgca cctgatatct atacagtgtg
    17161 ggaagattcg ttgacgaaca cgttggatgt atctggcagt gcagaacatt atgttgtatt
    17221 ttgggataac catcaactga cctataaaga aatatatagc cagcatgaag aagatttttc
    17281 tgaaggtaag gatattgtga ttgccgttcc ggagcaagcg aaatctgtaa aacgatcgca
    17341 aatcagaatt tatgcctaca ggggtaattt gatctccaat gatttattga taccattgga
    17401 atatggaaaa gtgattacgg attcgaaatt gcttgatcgc actgatttcc aaaaaaccat
    17461 tatgtatttt atgatggtcg acaggtttgt aaatggtgat aaatcaaatg atcatcctgt
    17521 tgatgatgct cgtgtaatgc ctcaggcgaa ctatcaggga ggtgatctgg cgggagttaa
    17581 gcaaaaaatt gatgatggat attttgagaa attgggcgta aatacacttt ggatatcacc
    17641 tgtatttcaa aatccgatgg gtgcttatca ggaatttccg gaaccacatc gatggtttag
    17701 cgggtatcat ggttattggc ctatttcatt aagtaaagtg gatgttcgct tcggcacaaa
    17761 tgctgaattc aaatctttgg tagacgatgc acacggcaac gacatgaata tctttctcga
    17821 ttatgtggct aatcatattc atgcagaaca tcccttgtgg acttcgcatc ctgaatggtt
    17881 ttcgcaattg gatcttccgg atggcaggaa aaatttaagg atgtgggatg aaaatcggtt
    17941 gacaacctgg tttgagccat atatgccaag tttcgaccat tcgcaacccg cagtggcgga
    18001 ggcttgtgca gatagtgcga tgtattggtt aactacattt ggcattgatg gtttcagaca
    18061 tgatgccact aaacatattg aaacggaatt ctggagatta ttgacattta aaataaaaac
    18121 agaagttgcc attccgcaac aaaaaccgat ttatcagatc ggagaaactt ttggttccag
    18181 agaattaatt ggcagttata ttggtagcgg gcttttagat gcacaatttg atttcgatct
    18241 ttattttgat gcaagagcag tatttgcttc cggtgatatt ccatttagtc gcctgtcatc
    18301 atccttgaaa gaaagttttg attatttcgg ttggcatcat gtgatgggaa atatcagcgg
    18361 caatcatgac atggctcgat ttatcagtta tgccagcgga gatctgaagt ggaatgaaaa
    18421 tgcaaaagaa gccggttgga accgtcatat tacaataact gatacgatag gttatgcaag
    18481 actgaaaatg ttgcaggctt ttaacatgac aattcccgga attccgatca tctattatgg
    18541 cgatgaaata gggatacccg gtggagatga tcctgacaac agacgtatga tgtacttcga
    18601 tagtctaaat cgatttgaga aagatgtatt caataccgtt tcgaagctca ctcaactgcg
    18661 tagcaatcat atggcattgc tgtttggcga tttcgaagtt ctgcaggaac agtcagccgg
    18721 tagcaatgat ttgcaatttg cttacctccg tagttatttc aatgatgctg tgattgttgt
    18781 attcaacaga agcagtaaac aggcaacgct gtcttatcca ttgcctacca ggttgttgaa
    18841 cgtaattccc caagcgcaaa tgggcacaac tataaacagg catggctacc agatcgatat
    18901 attggatatt cctgcaaatt cgtttgaaat aattactttt gatagataat tcacgttcta
    18961 aatttttatt aattacatga ccagaaattt tgtggcagct gtgatgctgc ttatgtcgac
    19021 tgttgcgctc aatgcacagg tgcaggctcc ggaatggagt aaggataaag tgatttacga
    19081 agttaatatt cgtcaataca caccggaagg aacttttaat gcttttactg ctcagttgcc
    19141 gcgtttgaaa caaatgggtg ttggcgtact ttggctaatg cctgttcaac ctattggtga
    19201 acttaacagg aaaggatctt taggcagtta ttactctatc tctaattatg tgacaaccaa
    19261 tccggagttt ggaaccttga aggattttaa gaaactagtt gacgaggcgc ataaattagg
    19321 catgtatgta atacttgatt gggtgggaaa tcacactgca tgggaccatc catggattac
    19381 tgatcatcct gaatattata caactgataa gaatggcaaa attcagccac cggttgccga
    19441 ctggacagat gtggctgatt tgaattacga taatatgcaa ttgcgtaagg caatgataca
    19501 tgacatggca ttttgggtga aggaaacgaa tattgatgga tttagatgcg atgttgcgat
    19561 gatgataccg gatgcattct ggcaatatgc cttcaatgaa atgcgtgaga ttaaaccgga
    19621 actattcatg ctttgtgaag cggaaggacc acagtttgtg aaagatggat ttaatatggt
    19681 ttatggctgg gaacgtcatc atgccatgaa tcagatagca aaaggaaaag gcacggttga
    19741 tcatttagat agtcttgtac aaaaagatat tgccagctat gcacccataa gttattatat
    19801 gaatttcacc agcaaccatg atgaaaattc atggaatgga acagaattcg aacgcatggg
    19861 tgatggtgca agagcattcg ccgtattgtc ggccacactc cctggaatgt tgctgatata
    19921 tagtggtcag gaagttggac ttgatcgtag attgaaattt tttgacaagg acagtattaa
    19981 ttgggttgat gataagcaat tcacgccatt ttatacaaaa ctgattaata tcaaaactca
    20041 aaatcaggca ctatggaatg gtaattatgg cggatcttat cgtcgcgttg catgtgaaaa
    20101 ttcaggcacc tatatttttg cccgtgaaaa agatggcaat aaagtattgg ttgttgctaa
    20161 tttgaccgcc gaaatttcta atattgtttt cgcgaacgaa gcaggaaagt acattgatgc
    20221 aatgtccggt aaaaaggaaa aagtaaaagc gacagagaaa atgacgctta aaccttggga
    20281 ggtgaaggtg ttggtcaaaa aataactttt agtaatttag ttgaataaca atcaatattc
    20341 atttctcgaa attaatttat atttttttgt aattaataaa atgatacata caaaaaaagc
    20401 gcttcactag aggcgctttt tttgttcaag tattacgagg tggttaccta attgctacat
    20461 cccttagtgc tgaatgacat tgcttgtgtt ttgctcaatc catcagcgca ttttgcagta
    20521 atgatgaatc gataggtggc acaaggggtc agattgtcga gcatgacgct tccgagattg
    20581 gtcatcagtg tgcctttttc tcgcggattg tttacattgt aaaactgcac tttataatac
    20641 gcagctccgt tcgtattcca atgtatggtc gcagaaatga gcgtaatctc gtcaacaaat
    20701 acacctaccg gtgcgccgca accgtctttc atttgtgttt ggtttccacc aacttgtata
    20761 tcattgcgtt gttgtgcttg catgcttacc agcatcaaca gcgaaattgt aagtgctatg
    20821 agctttttca tgactttgtg tttatggacc aaagttcatg ctattacata ggcacatcaa
    20881 tgaccaccga taccatcaaa cggtgattat cctcatcaaa aaatttttca gatctaaagc
    20941 aaccctctcc tctctccttt ctcctctctc ctctttcctc tctcctctct cctatctact
    21001 ataattaggt gcttcttttg ttattaccac atcatgagga tgagattcgc gaataccgga
    21061 agcggtaatc ttcacaaatt gagctgtttt caggtcttca attgttcgtg cacctgcata
    21121 tcccattcct gcgcgtaatc cgccgatata ttgtaccatg acttcttcaa cagttccttt
    21181 aaatggcacg cggccaacaa ttccttcagg aacaagtttt ttgaggtcat cttctacatc
    21241 ttgaaaatac ctgtctttcg accctttttc catggcttcc aaactaccca ttccgcgata
    21301 agatttgaat tttcggcctt cgtagatgat agtttcaccc ggactttctt ctgttcctgc
    21361 aagcagactt cctgccataa tggtatcagc gccggcaaca atagctttta caatatcacc
    21421 ggtgtagcgg ataccgccat cgccaataac cggaacgcct gttcctttca gcgcctgagc
    21481 agcgtcgtaa attgcactaa cctgagctac accaacacct gccacaaccc ttgtggtaca
    21541 gatgcttccg gggccaacac ctacttttac gccatcaaca ccagcatcgg caagtgcttt
    21601 tgctccggag cctgtagcaa cgtttccggc aacgatctcg aggtccggga aggtcttttt
    21661 caccaatctc accatatcca aaacgccacg tgaatggcca tgtgccgtat ctatgatcac
    21721 caggtcgaca cccacgctgc gcaaagcctc tacgcgttgg agagtatcgt gtgtaacgcc
    21781 aactccggca ccaactagca gtcggccaaa gctgtctttg catgctcttg ggtggttcag
    21841 tagcttttta atatccttat aagtgataag accttttagc gtgccggaag catctacaac
    21901 cgggagtttt tcgatgcgat aattgcgtaa aatgtcttcg gcttgagcta gcgtagtgcc
    21961 ttcgggagcg gtgataagat tttcttttgt catcacctcc gataccggtc ggctcagttc
    22021 cgtttcaaat cgcaggtcgc gattagtgag aatccccacc agtttttgat tatcgtcaac
    22081 cacaggaatg ccgccaattt tgaattcgcg catcatttgg agtgcatctc ttagtgtagc
    22141 cgttttcgac aaggttacgg gatcgattat gagtccactg tcgctgcgtt ttacacgacg
    22201 cacctgatcg gcttgttttt cgatggtcat gtttttgtga atgatgccga tgcctccctc
    22261 acgagccatt gcaatagcca tttgcgattc ggttacggta tccatggcag ccgatacgat
    22321 gggcacattg atacgcagat ttcgagtaag gcgtgtagaa atatctactt ctctgggaag
    22381 gacctcagag taggcaggta caacaagaag atcgtcgaaa gtaaggcttt cgcccagaaa
    22441 cttggttgag agatgttcca tgtgcaaaga tacggcatat ttttcgggag atgcaaattg
    22501 ggggtgtttt gacccttaaa atgagccccc agatcttaaa cctaacaggt cttagacacg
    22561 aaaggtttag agcgatgaag cgcttatatc cgaatcaaac cgcttagata tctcccactc
    22621 tctctcccaa aatttctata tttttaatac atgaaaacct tagcgttttt cctcgccttt
    22681 tcttatttat cctcaagact tttctcgcag gtctatatta ttcctaatgc cgaattacaa
    22741 ccatcttgga ctttcccatt gtgttttgaa gacgcctccg gcgccagaga tacaatatat
    22801 atggcctacg atattgatgc aattgactat agcattcctt cagccgacac catattcggc
    22861 gaagcgtttt taccaataga tacttcggaa tttagagcct attgggagta tatttacgat
    22921 ggaaaagcag tgaagacgct tgtaatgaag aattttattc ctgcacaaat taattttaat
    22981 aaggcacagt tgcccgtgaa atttacatgg gatgctcaat tgttttattc cgaaaaccta
    23041 ccatttccaa acaactaccc cttgccaaat gcatggggat tggtccattg cagttcttgg
    23101 aaagattttg aaggttgtca gctggatgaa tactccggca tatatctgac caatgaaccg
    23161 gattttgtgt atacagatgt tgctacagac agtatattgt atcaatcaga atattattat
    23221 ttttttgaga atgggttaga ctttcgaata ctccaatatg gtacaggtta taattgggca
    23281 actattgttg aaaatgaatc ccagtcattc caaatttatc caaatccgag tgacgatttt
    23341 atacatattt taacaaagga aaataccaag gccacattaa ttattagcga tatgcaggga
    23401 aaaacaatca agcagtttgt cgatgtatct aataccacta tccctgtcta tgatttgttg
    23461 cccggaatgt atatggtaaa ctatagtagt tatgaatctg cttattcgtc aaaatttatt
    23521 aagaagtaat caagaatctt taattttcaa ttagttgtaa aagtatcaat ccttttgcaa
    23581 tacaaccgtt tttgctacat gcatttattg tataagattt tctattaaga aagttttaca
    23641 ctttattaga ccccggacta catttgattt gcaaaacttt ccgttaattt gaattgaaat
    23701 tattcatcat ttatccaaca tgattccacg ccggaacggc atcctgctcg tcgaaacatt
    23761 tacaatcctg attttgaatg gggtgttggc aacctacatg cattcgcatc ttccaactac
    23821 cctgttccga tgttgttatg cacccttgct attatttcag ggactgtggt attatcgcat
    23881 ttatattgtt ggacatgaag cagctcataa aaaattaatc cctgcacatc ttttattgaa
    23941 tgaaatagcc ggatcattag tgctcctgcc attactgaca cccttaaata tttatcgcaa
    24001 aattcactat ttccaccatg gattcaatcg caaagatcct catacttctg ccttagatac
    24061 attcgtggta aaaggaaaat atcgcacatt gaaaactgca tattattatg tcgtttggta
    24121 tttaagtgtg ttttgcggcg gctttttttt acattcatta gtatctgtaa ttttattttt
    24181 gttctgtccc cccgcactcg cccgaaaaat atctccggca ttcaatggtt ggaattataa
    24241 agatcaatta aaagctatat tgttatttct ggccggaatt ttaattcatt tatgggtatt
    24301 tacattcttt ggtaaaactg cttatttgct gagtcttgga ttacctatgc ttgtctttgc
    24361 ctggctgttg tcaatgctcg tttatgtttt tcactacgac acaacaatgg gagagcatgt
    24421 tcgatataat gtgcgctcgg tgaatcgtat acctgtttta tcctggatac tcatgaattt
    24481 caatgaacac gctacacatc atcaatatcc gaatatacca tggtacagtt tacctgaaaa
    24541 tcgctccgaa ttgcctgagg agtttggaaa taaaaaccaa tctacccgaa atttatttgt
    24601 agccgttatt cgtcaattaa agggacctca tgtcatatat caaccttaga tacggcaagc
    24661 aggaggatgt ggatgctatg atgtacatta agcatcaact ccctttaaaa acagccgatg
    24721 gaaatcctgt taaaggtggg tttttacttg gcacgacccg ggaacaatac ctctcattta
    24781 ttttaaatga tcattgtctc gtagctgaag atcatggaaa tgtagttggt tttggtatca
    24841 tgttacataa tgcatcactg cgtaattccg atatttggca aaggcgacat caggctgcat
    24901 gggaagtaga tattaatgat tttgaagatc tgccaatatg ctattttgaa caactcgctt
    24961 ttctgccggg ttataataga gccgttttga aattagcata tcaattggtt atggacgcat
    25021 ttcgccaggg acacacacac ttatttgcta ccaccgtaca caagcctgtg ttaaatattg
    25081 cagcagtgcc ttttataaaa aaggctgatg gcggcctggt ggggagaata gatgaatatt
    25141 atccggaagt gggaaatata ctttccgaca tttatctgat agataggatt aagttttatg
    25201 atgcagtgcc caaactcgca atttatccat ggttgatcaa gagtcttgcg ggtgatagta
    25261 aattggtgta aacactatca ttctttgtat tactgatcat tttgtataga tataattgca
    25321 atcttactgt taatttatgt tgtcgttcac attttatgat acctgtgctt tatatttggt
    25381 gtgataattt taatatgaaa tcattactta taattggagt gctgacattg tcttgcatga
    25441 gcaaattgca tgctcagaaa attaatcctg actatgattc gaccttagct aaacaattga
    25501 atgccgatga ctatggcatg aaatcctata tactggtgat tttaaaaacc gggtccaata
    25561 atgttcagga taaacaactt cgcgatagtc tcttcgccgg gcattttgca aatatgtctg
    25621 ctatggtcga ggccggtaaa ctggttgtag cgggaccaat cgacaagaat gaaaataatt
    25681 atcgcggaat tttcatccta aacgttacct cctttgaaga tgcccaatta ctacttcaaa
    25741 atgaccctac tattcatgaa aaagtgctcg aagctgaact atatgaatgg tatggatcag
    25801 cagctttgcc cgtttatctg gatacacatg ataaaataca gaaattttca atagagtaga
    25861 aaggagaaag gagaaaggag aaaggagaga ggagaaagga gaggggtgaa aggggaaaag
    25921 gagtaattat tggggctgcg ttgggaatga ttgcatgagt tgttgaatta aatccaaaaa
    25981 tcctccgggt ataactttaa aattcaatct atataatatc ttaaaatgga tctcagcttc
    26041 ttgcggttat gttttcaagc aatattatcg ttcatttttt tcatttgtgt gagaaacacg
    26101 caagcgcagg tcctgcaggc tgatacactc ccgcaatctg aatacttgaa tttttatatc
    26161 agtgcgagca gcgaagattt ctacatcaaa tttacacaag tgcctaacaa gctctttggt
    26221 gagagtttga aagtgcgaat aaacaatgaa tcaggcgttt ttgcaattcc gatcattaat
    26281 gaagaaaatt acagtattga tttacccggc agatcaagtc cctataggtc tgatacactc
    26341 tacttctatg ttgaaatgta cgaccaggta tatcattcta ttgctgtggt aatattcccc
    26401 ggttttatgc gcaatatctc ctttggtgag ggctgctatg aactcgattg cacaccggta
    26461 caggatccct atatgccttc tgtcgtcgta ccattcctca tcgaccccga tgctgcctac
    26521 aagatacccg gaaggaacaa gatcattacc ggcaaaatgg cactcagcag gtatactccc
    26581 tgcgatttag aaaaatttga gcaagtgttt tttcaggata attagtgctc aatgagcatc
    26641 gccgcatccg gtgataactc aaacttcatc atctctcagc ggattcctgc ctctccaccc
    26701 tctccgcgct ttcgatctca accacaccat cattgtctga atcacggatt atttggatta
    26761 aaggattaca cggattttcg gtataggcac attccacaat caacccacac ccgaacccga
    26821 acacctgaac acaaccattg tctgaatcac ggattaatgg gattaaagga ttacacggat
    26881 tttcggcata ggtacattcc acaatcaacc cgcacctgca cccgaacaca attagagtgt
    26941 ggaagtcccg cttaggcggg atgtcgctgc gctccatgtg caagcctgcc tcgccgc
//
LOCUS       DAOQOU010000025        26654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_838_length_26654_cov_6.111696, whole genome shotgun sequence.
ACCESSION   DAOQOU010000025 DAOQOU010000000
VERSION     DAOQOU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26654
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_838_length_26654_cov_6.111696"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..402
                     /locus_tag="PLM90_03795"
     CDS             16..402
                     /locus_tag="PLM90_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ88522.1"
                     /translation="MKPNEVSRIIIEEAIYIHRKIGPGLLESVYEKMLAHRLIKRGLI
                     VKTQVPVPVVMDEVQLEIGFRADMIVENCIVVEIKSLDLVPKVAFMQLKTYLRFTNIQ
                     LGLLINFNVPLLKDGVTRFVLDFKEE"
     gene            601..1647
                     /locus_tag="PLM90_03800"
     CDS             601..1647
                     /locus_tag="PLM90_03800"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HMZ88523.1"
                     /translation="MHDRISRKNTGYVIPNLFMEIYYKFRLTICFVLCLALFPNNLKA
                     QSLEHFVTPSDTFNTKRFGLILGSETALYSAATIALYQYWYKDYPLNHFHLFNDNGEW
                     LQYDKMGHFYTAYFETNLTTEFYQWTGMSDNAANWSAAATGSILQLTIEVFDGFSEKW
                     GFSVGDFAANTFGATLCTAQNMLWDEQRIKVKFSSHFTDYSGYDQPVQDRAAQLFGTT
                     GPEKVLKDYNGQTTWLSVNPSMFMSPETRFPKWLMISGGYSVDGLLGGYENKWCDDPE
                     MKPEDCPPDQLTDYSQIPRERQFYLSLDVDLTQIPTNSPYLKMVFNVLNIVKFPAPAL
                     QLQNGNLSFKPIYF"
     gene            complement(1665..2318)
                     /locus_tag="PLM90_03805"
     CDS             complement(1665..2318)
                     /locus_tag="PLM90_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ88524.1"
                     /translation="MKRTIKWNIAQKAELKWWQQYLKGKDVEAYHTWKRAYWQGILDK
                     IAGSAPIREGMSILDAGCGPAGMFMQLPNCNVDALDPLLDEYEKNLPHFKRSMYANTT
                     FHALPLEQYQTDKQYDIVFCMNAINHVSDIAFSYDLLAKMVKPGGKLVVSIDAHNHNF
                     FKHLFRMIPGDILHPHQYDLKEYSAFITDRNCRILQTEKLKHEFFFDHYVQVAQKIS"
     gene            2355..3323
                     /locus_tag="PLM90_03810"
     CDS             2355..3323
                     /locus_tag="PLM90_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMZ88525.1"
                     /translation="MQIPVGEQVVILDSGIIGVIEYYNGSTAYINVNGEIHQAHKKDI
                     ASVREFGGKGKSTIKKEELSQAAKDIGLFIHWRAVKNMGGDVDRFEVELVNNTPLRLL
                     FEYKWYLLEELQTTVRNTITPNATIRLHGMKPDAINDRPVSTLQCWRQDDKGITISVA
                     DSEVKLRPKQYVTKLSTEPYLSSGIIVFPVNVSDSSLQIPASEKKPTPAFNFNEKKKV
                     PVINEVLQKAAMPDYIDLHVEKLLPSHKHLSSTEILAMQISQFRHFLEKAIRLKLHRI
                     YAIHGHGKGVLRTEIEKTLKKYPEVTSYNNDYNPRFGHGATEIILD"
     gene            3402..3790
                     /gene="rnpB"
                     /locus_tag="PLM90_03815"
     ncRNA           3402..3790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM90_03815"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3811..4032
                     /locus_tag="PLM90_03820"
     CDS             3811..4032
                     /locus_tag="PLM90_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88526.1"
                     /translation="METLKNVILCPFCGHLSQMLWVHGHGQCAICGMTIDECCRGEQA
                     CEIEDPQKRKAKKKITPQKEEPNNETTKP"
     gene            4013..4822
                     /locus_tag="PLM90_03825"
     CDS             4013..4822
                     /locus_tag="PLM90_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HMZ88527.1"
                     /translation="MKLLNLKSALLMLACCLGIQFASAQNLVTFAPPEPPKPAVPVNI
                     DTVITTIDAAKGYKRITITANNIVVSDGYMIGNKKTGTWSTFQLNGSLLTLAEYDNDV
                     KSGNYLEFDKNGNLLVLEGYKNGKLHGDQKKYTQGKTGRVLKSSYGYADGLYHGDCIE
                     YTVDGLIQSSMHYANGKKDGTSRWYFSSGKVAMEQHYTNDQLNGKQLIYDQTGALISE
                     GTYTNNMKSGTWNENYPNGKLKSTGQYTNDKKTGEWKYYKEDGSLEKTEKF"
     gene            4963..6318
                     /locus_tag="PLM90_03830"
     CDS             4963..6318
                     /locus_tag="PLM90_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ88528.1"
                     /translation="MASLLLIDDEKSIRKTLKEILEYEGYTVDEAGDGAEGLRMIQDA
                     DYDIVLCDIKMPKMDGMEVLEKAQITNPDVPFIMISGHGTLETAVEAVKKGAFDYVAK
                     PPDLNRLLITIRNALDKSNLITETKVLKRKVSKTREIIGDSPAIMKIKETIDRVAPTD
                     ARVLITGDNGTGKELVARWIHERSNRASGPLVEVNCAAIPGELIESELFGHEKGAFTS
                     AIKQRIGKFEQANGGTLFLDEIGDMSHNAQAKVLRALQENKITRVGGDKEISVDVRVV
                     AATNKDLLKEVESNNFRLDLYHRLSVILIHVPSLNDRKDDIPLLAERFVKEICEDYGV
                     PKKVVTPKAMIEIQNINFTGNIRELRNVIERLVILSDQKITDEDVLNYAQSPKSGGSP
                     KDLYERFEKFQDFKDFVEKEFILHRLNKNNWNVSKTAEELDIQRSHLYNKIEKYNLKR
                     D"
     gene            6330..6506
                     /locus_tag="PLM90_03835"
     CDS             6330..6506
                     /locus_tag="PLM90_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88529.1"
                     /translation="MAANKPKAQSKTDQMPKDKPMANESQENIVTNAANMLIGIGSVV
                     IMALIIIWMIKLLG"
     gene            complement(6600..7535)
                     /locus_tag="PLM90_03840"
     CDS             complement(6600..7535)
                     /locus_tag="PLM90_03840"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HMZ88530.1"
                     /translation="MSFAVTVIGSNSAIPAHGRHPSAQVVTINDRLYLIDCGEGTQMR
                     FNELGIKWMKINQIFISHLHGDHFFGLIGVLSTYHLMRRNRPLDVYAPAPLADIIKMQ
                     LEAGESQLNYELRFHELDTVNTGTIYENEDITVDTIVLNHRIPCVGFVFREKQRDRKV
                     IREKLQEFQIPVELIPDIKKGMDILDEKTGNRILNKDITIDPPLPRTYAYCCDTAYYP
                     AMVPFIANADLVYHDCTFDEASSQRATDTFHSTTKQAASIALQANAKKLMIGHFSSKY
                     DDLLPLLREAQEVFSNTILAIEGKTVELERVTGTP"
     gene            complement(7540..7911)
                     /locus_tag="PLM90_03845"
     CDS             complement(7540..7911)
                     /locus_tag="PLM90_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ88531.1"
                     /translation="MKFTVDKKERYCIFKLDEPRLDSILSANLKSELVLLNQEGFRNI
                     ICDLSEVNFIDSSGLSALLIGHRICKDSNGTFVLIGCGESTIKLVKISQLDSILNIVP
                     TESEATDFILMEEIERELGNE"
     gene            8129..10732
                     /locus_tag="PLM90_03850"
     CDS             8129..10732
                     /locus_tag="PLM90_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HMZ88532.1"
                     /translation="MDAKFSPKVKEVISYSREEALRLGHDHIGTEHLLLGLIRDGDGL
                     AIKVLLSLDVDIAMLRKTVEDAIRDKASRQPFNAGSLPLTKQAEKVLKITVLEAKVLK
                     SDTIGTEHLMLSILKNKDNVATQILGQFDVDYDVFRQELDYVRQDQGQREIPPTNQNE
                     DENFEEEEKGDRGFNVPRKPGAAKSKTPVLDNFGRDVTRAAEEGRLDPIVGRQEEIER
                     VSQILSRRKKNNPILIGEPGVGKTAIVEGLALRIIQRKVSRVLFDKRIIMLDLAALVA
                     GTKYRGQFEERMKAIMNELEKNRDVILFIDEIHTIVGAGGATGSLDASNIFKPALARG
                     ELQCIGASTLDEYRQYIEKDGALDRRFQKVLVDPPTAEETMEILGNIKSKYEEFHNVN
                     YSDAAIKACVTLSTRYITDRYLPDKAIDVLDEVGARVHLKNIHVPDEIVKLEGEIEAI
                     KDEKNKVVKSQRYEEAAKLRDTEKRLLEDLDRAKTKWEEHVKSERYPVTEDDIAEVVA
                     MMTGIPTKRIAQSEGTKLVGMADELKRGVIGQDDAIVKITRAIQRNRVGLKDPKKPIG
                     SFIFLGPTGVGKTELAKVLSEYLFDSKDALIRIDMSEYMEKFSVSRLIGAPPGYVGYE
                     EGGQLTEKVRRKPYSVILLDEIEKAHPDIFNILLQVLDDGILTDGLGRKVDFKNTLII
                     MTSNIGARQLKDFGTGVGFSTAAKQLAQEENTKSVIETALKRTFSPEFLNRIDDVVIF
                     NSLSREDIFRIIDISLRDVTKRLETLNYKLQLEESAKEFLSERGYDPQFGARPLHRAI
                     QKYLEDPLAESILGGKFKEGDTLLVEFDKDLGGLVINPAGEKEAEKEAEKEKKKGKGK
                     DKKEEREEEKA"
     gene            10880..12427
                     /locus_tag="PLM90_03855"
     CDS             10880..12427
                     /locus_tag="PLM90_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88533.1"
                     /translation="MKFAILYALMLMSTIASAQADFEWYASYNNVTFSSFGYPKDIVL
                     DNEGNVVACGYTAINDTSQSLMTIIKLSPAGDQLWNFNMTLSDEYPALVTALDVNSNN
                     EILFTGETGDTMIVGKLNPDGLLQWIEKYAINKYWNHGRDIHVTATDEILVSGDCSND
                     TVVLLKYTNDGDLLWERFRQRQFYFGFVYTAFDSESNTYQCYEVPGTDEYTDFEVCKY
                     NAEGDLMWAHAYSNFSLTAFARDFAISDDGCAYVLNRAYGNSQLYELVKIDSVGDLAW
                     SAYLDNFPDQNYFTYATDIAVDKDKNVMVIGEQYDSDLLVDYKNIVKFNDDGAYVWQK
                     GVDYIANTVSYGSDIFTDNYGNIYSMFNYGDTIPWSSTYMMINKRGPSGDLKWSATFS
                     ENCYPEQLLVNEDEVYALITSPGDNLYDAIGVIKYSQVVTMQSQAPQTIAMQAYPNPA
                     IDYIQFMANANVELMHAETYNIAGELVDVNIKLNTPCDVGMLPAGYYTSRCITSLGTA
                     TIAWVKQ"
     gene            complement(12456..12992)
                     /locus_tag="PLM90_03860"
     CDS             complement(12456..12992)
                     /locus_tag="PLM90_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88534.1"
                     /translation="MNRKLLFLLLSSILVFSASAQQTQKLRKHYYILIDDTTFVNAMP
                     EQGATLKAYFKNDTIYKIETWFGFNFGEVTRDYYYWDDTLSLVNETQKLYNAEATPKI
                     NPDTIKTSYAGRYIFQHNKLTGISQKGSYSISDTPQTKEDTQAALLMLSDKYRVLAYE
                     KRKAKKNRVKIKSNELVK"
     gene            13047..13658
                     /locus_tag="PLM90_03865"
     CDS             13047..13658
                     /locus_tag="PLM90_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMZ88535.1"
                     /translation="MAGLREKLLAPDNRVRRNPMVIVVNMLLASIVVLFLALTYGYAL
                     RMGSNWLEFRLPKVFWLSTICIMLVSFFLRRTIKYYDTDRTARLRRAMALATIMATAF
                     VVCQLIGWGELGKAGILMDTSTSASYLYLLSGLHLLHVGVGIIILLVGCVRLFTNTST
                     PVKALLYYADPVRRDRLKLLNHYWHTIDFLWLFLFLVILYNQT"
     gene            complement(13732..14157)
                     /locus_tag="PLM90_03870"
     CDS             complement(13732..14157)
                     /locus_tag="PLM90_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88536.1"
                     /translation="MKKLLMTLSFVLAVTAGQVFAQSAPSGDANKEAKKTEVKKVAPE
                     KRQAITPAQAKKKAAMNGKMNAKPAKARVKAHQDEIQAHDASKAEVKAHAQDAKANKT
                     EADKKEAVKAHKDEIKAHADSKEEAKAKLQRKHDKKKNE"
     gene            14330..14406
                     /locus_tag="PLM90_03875"
     tRNA            14330..14406
                     /locus_tag="PLM90_03875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14364..14366,aa:Pro,seq:ggg)
     gene            14612..16645
                     /locus_tag="PLM90_03880"
     CDS             14612..16645
                     /locus_tag="PLM90_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HMZ88537.1"
                     /translation="MQFVYPAFLFALATLAIPVVIHLFNFRRFKTIYFSNVRFLRNVQ
                     EETATKNKLKHLLVLLTRCLALAALVFAFAQPFIPAKKSGNNSSDRAVSIYIDNSFSM
                     EAEVNDERLIDIAKRKAEEIVNGFTIDDRFQLITNDLEAKHQRLVSKDEMIRFIAEVQ
                     PSPVVRPLQEISDRQRDALHREGDKAEKISYLLSDFQKNAGDIDNDTSIIVNFVPLLQ
                     RAQKNVAIDSMWFTTPVPVLNQPISICIRLHNYGDGDVENVPVTLRLNEQIKAIADIN
                     INAKGDVVDTMTFTLNSGGWYSGEISIKDYPVTFDDMLYFSFKPVEQIPVLAINGGSE
                     NIFLKGFFGNNNLFQLKNNPASQIAFSELPTYDLIILNEVPSISGGLADALQQHLARG
                     GNIMVWPSLTMDVASYQAFLLANNAAVYGALINRPRPVSEVDTKNPVFADVFEKIPRN
                     LAMPTAMKSYALQTSTGTREEMLLRFTDRQAMMARYASGDGYIYLSAVPLDRTVTDLP
                     VQAGLFAPIMYKVAVSNARRLPAYANIGEMGWINLPGIQLPGDATIKVESKGNEFIPE
                     IRRTGNSVAVNLSSYVQQAGWYTIVPDKQMVNSDQLLALNYNRSESDMSFADASALKE
                     LYAASNQEIISDIDRNFTGMVTQMATGTPLWKYCIIFVLVFLAAEIAIIRLMP"
     gene            16658..17923
                     /locus_tag="PLM90_03885"
     CDS             16658..17923
                     /locus_tag="PLM90_03885"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ88538.1"
                     /translation="MHCILQGATIIDPASQHHRSTVSIRIQNGVITEIASSIADTGDA
                     QLIDLKGNFVSPGWMDMLAAFGDPGHEYRETIQTGAAAAAKGGFTALGLMPNTQPALH
                     GKAEIEYIKNHAAGQLTHIFPYGAVTHNREGKDLTEIYDMHAAGAMAFTDADSAIRDA
                     GILLRSLQYVKPFNGVIINIPNDHKIVGNANVNEGAMSVQLGMYGIPDILEELMVIRD
                     IKLAEYTQSHLHIGIVSSAKVLPHIRAAKEKGLSVTAGVAAYQLYFDETELHDYNAHY
                     KVNPPLRTAADKQALREAVADGTIDVVCSYHLPHDNDSKDVEFEYAAFGMASLEVVFG
                     AMRSALPNISPEQIVNILAAKPRNILNIAQPGIAVGNTADITMFDAETTWTLQQSDLR
                     SKASNNPFVGKTLKGKVLGVINKGKISIA"
     gene            17925..18473
                     /locus_tag="PLM90_03890"
     CDS             17925..18473
                     /locus_tag="PLM90_03890"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HMZ88539.1"
                     /translation="MEGQDKIMDVQPIERPSRKGLILRNGLIFVALQIAIMLVTHFRR
                     DSISDQNMRIAVGMYLLLLAAIVVVQLDARMRVYSGRMDFGQAFSTGMIFVLFVTALF
                     TIFILVFYLTIGKELLAQMITIAENSLRANGTAEADIPERMEFSRKIFTAPGMSIITL
                     IGYLFFGTIMSLIGALFTQRTN"
     gene            18473..19429
                     /locus_tag="PLM90_03895"
     CDS             18473..19429
                     /locus_tag="PLM90_03895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ88540.1"
                     /translation="MQISVIIPLLNEEESLPELSQWISKVMQENNFSWEVIFVDDGSR
                     DKSWQVVQQLHADNASFKGIKFRRNYGKSAALNEGFAMAQGDVVITMDADLQDSPDEI
                     PGLYKMIADEGFDMVSGWKKKRHDPIGKTIPSKFFNWTTARITGIKLHDFNCGLKAYK
                     RDVVKSIEVYGEMHRYIPQIAKTAGFYKIGEKVVEHRARKYGYSKFGWERFMKGFLDL
                     TSIMFVSKFGKKPMHLFGTFGFLSFFTGGIITIWLIIDKIIKMNTGEKFRQVTDQPLF
                     YIALVAMIVGVQLFVGGFLGELISRNSPDRNQYQIDQRIGGV"
     gene            19426..20544
                     /locus_tag="PLM90_03900"
     CDS             19426..20544
                     /locus_tag="PLM90_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88541.1"
                     /translation="MKILLIGTAWPYRGGLASFNERLIQEFITEGDDASIITFTLQYP
                     GFLFPGKTQYSSSPPPGIPITRKVNAVNPFNWWRIGKAIAKEMPDIVIIKYWMPFMAP
                     CFGAIARQIKKNKHSSIICIADNIIPHERKFYDKPFTKYFISAMDGFVLMSKSVMEDL
                     AQFDREKIRALNPHPLFDNFGEGVSKTEALAHLHLEANRRYILFFGFIRDYKGLDILL
                     EAMHAIPEDVHLIIAGEYYTDSKPYEALINQNNLGDRIHAFNRFIPDEEVKYFFCAAD
                     LVVQPYKHATQSGVTQIAYHFEVPMVVTNTGGLAEMVLDGQVGYVTPAEPLAIAQAVS
                     KFYVEGKSEEFKRNLREEKSRFSWSRMTSTIRRLYQKG"
     gene            complement(20555..21874)
                     /locus_tag="PLM90_03905"
     CDS             complement(20555..21874)
                     /locus_tag="PLM90_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ88542.1"
                     /translation="MSIQPQLKRFDLTMIVVSLVIGVGIFRTPAMVAQSAGKEWIFYA
                     AWITGGIISIFGALTFAEIGARLPSAGGYYRIFSTAYHPIYAFMLNWSQVIINAGSSA
                     GVAIIGAEYLTPVLFGNTAEDYVVIDTAAIVIILILFGINYAGIKMGARAQNVLSLLK
                     IVMIITCCFAIFSGQANADIPVVIDEDWNFINAFGIALISIFFTYGGYQQTVNFGADV
                     KDPQKNIPLAIFFGVGIVIVLYLLINLAYVNVLGFEQMKNTPLIAKDLAEALFGNTGA
                     VITSIILFTSVLGFLNTSMMSNPRVYYAMAEDKVLPQIFSKVNHKTQVQEFGLSFFVA
                     VMIASVFLLGNFENIVTYVLFIDSISLASAAGALFIFRHRSPKDYSGFKMKGYPYIPA
                     VFMLVLLFVTYSTFMDDATSAQYGFLIFLMGLPLFFLITFFSNKKQQ"
     gene            complement(21983..23284)
                     /gene="icd"
                     /locus_tag="PLM90_03910"
     CDS             complement(21983..23284)
                     /gene="icd"
                     /locus_tag="PLM90_03910"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HMZ88543.1"
                     /translation="MSGQKITIQNGVLNVPDEPIIPFIEGDGTGADIWAASVRVFDAA
                     VAKAYGGKRKIHWMEVLAGEKSFNQTGNWLPDETLDAFREYLVGIKGPLTTPVGGGIR
                     SLNVALRQILDLYVCLRPVRYFEGVPSPVKSPEKVDMVIFRENTEDIYAGIDWAAGSD
                     LVGKFLNWLQENSPKDFEKIRFGNKAKTDAWEEALRSVGVPNRDVDVQVGIGIKPVSY
                     SGSERLIYSALDYAVQNNRKSLTIVHKGNIMKFTEGAFRDWGYQVARDFFGAVPLDGG
                     PWHVIPEGKPGAGIVIKDAIADITLQQVLTRPDEFDVIATLNLNGDYLSDALAAQVGG
                     IGIAPGANINYVSGHAIFEATHGTAPKYANQDKVNPGSVILSGCMMLEYMGWKEANDL
                     IIQGMEKAIAKKRVTYDFHRLMEGATMLKCSEFGDEIIANM"
     gene            23407..24861
                     /locus_tag="PLM90_03915"
     CDS             23407..24861
                     /locus_tag="PLM90_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ88544.1"
                     /translation="MNNKPQLVRSLGLSTAILLVISSMIGSGIFKKIAPMSEQLPDSN
                     LILLAWLLAGVVSMLGVFTYAGLSSMTEEGGGQFEYFRIIYGKFFGYLFGWTCFAVIQ
                     SASIASIAYVFSQSANNLLPMGDPLAAWADISIAGKIFPFSNSGVKIFTIITIAVLTL
                     INYYGVKKGGLLNDVFSAAKIAGIVFLIIAGLALHGTVTEDASINVPELPQGLSLLST
                     MFAAMLAAFWAYDGWINISYIGGEVKNPKKNIPIAIIGGTAVVMLLYTLVNYVYLHVL
                     PVSSFIAIKNSDNQIAAAEVAQHLLPGFGFTLISILIMICTFGATNASLMSSPRIYHQ
                     MAKKNLFFKSFGETHPKYHTPHISLFYQMIWSSVLVISGTFDQLTDMLIFASFIAYGS
                     GAAGLLYMKYVGKKIQMPDGTVQHQRITAKVIGFPVIPIVFLLFCIALVGNTLFTYTS
                     ESLTGLAFIAIGIPLYFVFQKSKNRSIDPIDTLE"
     gene            complement(24961..25857)
                     /locus_tag="PLM90_03920"
     CDS             complement(24961..25857)
                     /locus_tag="PLM90_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88545.1"
                     /translation="MKKYLIVFAFAPLMIACNQDKLDALEQENADLKNKSAEFKSGWD
                     AAVNEISSYMSMMNEIDSNLIEIKKTEGMIDNSLHSEGRSKEQTREEIMANIATLNDL
                     IQKNKNLVKDLDRKNKGNNLKIKELDERIALLNATLEQKEQDLAALRAELEAAKYQIT
                     NLNTDLATVTSAKQLLEEQNTQQAKVISDQTDVLNTAYYISGTFKELKELGIVDKEGG
                     FIGIGANKELTNNFDASAFTKVDIRKFKELALNSKSAKVITTHTSDSYTLVEGDKTIE
                     ELVIENPDEFWKASKFLVVLTD"
     gene            26048..>26654
                     /gene="ruvB"
                     /locus_tag="PLM90_03925"
     CDS             26048..>26654
                     /gene="ruvB"
                     /locus_tag="PLM90_03925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMZ88546.1"
                     /translation="MKNPNLEPTDDFMSGSDKELEKVLRPQGFYEFTGQERIIENLKV
                     FIAAAKQRGEALDHVLLHGPPGLGKTTLSFIIANELGVNMKITSGPVLEKPGDLAGLL
                     TSLEERDVLFIDEIHRLSTVVEEYLYQAMEDFKIDIMIESGPNARSIQINLNPFTLVG
                     ATTRSGMLSAPLRSRFGISSRIEYYSAQTLQQIVTRSSGILQ"
BASE COUNT         8054 a         5391 c         5589 g         7620 t
ORIGIN      
        1 ctataaattt tagtgatgaa gccgaatgaa gtgtcgagga taatcattga agaagcaatc
       61 tatattcacc gtaaaattgg acctgggttg cttgaatctg tttacgaaaa aatgctagcg
      121 catcgtctga taaaaagagg actaattgtt aaaacacaag tccctgtgcc cgtcgtgatg
      181 gatgaagtgc agctcgaaat tggattcaga gctgacatga ttgttgagaa ttgtattgta
      241 gtcgaaatta aatccttgga tcttgtgccg aaagtggctt tcatgcaatt gaaaacatat
      301 cttcgattta caaatattca acttgggcta ttgataaatt ttaatgtacc tctattaaag
      361 gatggagtta ccaggtttgt attggatttc aaggaagaat aaaatatagc ataagaagta
      421 atagttcaaa acatttgaat atgcctacct ctgcgccact ttgcgtccat tgcgcttaat
      481 caaataacgt gcataagtaa agtcagttgt cgctcgtatc atcgttctca cccatgtaat
      541 taatgggcaa tttgctgcta ctcaaagtga attaataaat ttgtaatttt ggcgaccaag
      601 atgcatgatc gaatttcacg taaaaatacc ggatatgtca tcccgaatct atttatggaa
      661 atatactata aatttcgttt aacgatctgt tttgtgttat gccttgcctt gttcccaaat
      721 aatcttaaag cgcaatcgct tgagcatttc gtaacaccat ccgacacctt caataccaag
      781 cgctttggcc ttatattggg atctgagacg gcattatatt cggctgctac aatagcactt
      841 tatcaatact ggtataaaga ttacccgctt aatcattttc acctctttaa cgacaacggc
      901 gaatggttgc aatatgataa aatgggtcac ttctacaccg cttatttcga aacaaatttg
      961 actacggaat tttatcagtg gacaggtatg tccgacaatg ctgctaattg gtctgcagca
     1021 gcaacaggtt ccatacttca acttaccatt gaagtgttcg atggattttc cgaaaaatgg
     1081 ggattttcag tcggcgactt tgctgcaaat accttcggag caaccctttg cactgcacaa
     1141 aatatgttgt gggatgaaca acgtataaaa gtgaaatttt cctcgcactt tactgattat
     1201 tccggttatg atcaaccggt gcaagacaga gcagcgcaat tatttggaac tacaggaccg
     1261 gaaaaagtgc tcaaagatta caatggtcaa acaacgtggc taagcgttaa cccttcaatg
     1321 tttatgtcgc ctgaaacacg ctttcctaaa tggttgatga tatccggagg ttatagtgtt
     1381 gatggcctgc tgggaggtta tgaaaataaa tggtgcgacg accctgaaat gaaacctgaa
     1441 gattgccccc ctgatcaatt aactgattac tctcaaattc cgagagaacg ccaattttat
     1501 ctttccttgg atgtggacct cacgcaaatc ccgacaaatt ctccttacct gaaaatggta
     1561 ttcaatgtgt tgaatatcgt caaattcccc gcaccggcat tgcaattaca aaacggcaat
     1621 ctcagcttta aaccaattta tttctgataa aagggaatta acctttaaga aattttttgc
     1681 gcaacctgaa cgtagtggtc gaagaaaaat tcatgcttga gtttttccgt ttgcagaatc
     1741 ctgcaatttc gatcagtgat aaatgcactg tattctttca aatcatattg atgcggatgc
     1801 aaaatatcgc cgggtatcat gcgaaataaa tgcttgaaga aattatgatt gtgtgcatca
     1861 atgctaacca ccaacttgcc accgggtttt accatttttg ccagcagatc ataactaaat
     1921 gcaatgtccg aaacgtgatt gatggcattc atgcaaaaca caatatcata ttgtttatct
     1981 gtctgatatt gctctaaagg caaagcatga aatgtggtat tggcatacat actgcgcttg
     2041 aaatgaggta gatttttttc atactcatcc agcagtggat ctagtgcatc aacattacaa
     2101 ttgggtaatt gcatgaacat cccggcagga ccacaccctg catctaaaat gctcatgcct
     2161 tcgcgtatag gcgcagagcc tgcaattttg tctaaaattc cctgccaata agcgcgtttc
     2221 caggtatgat aagcctcaac atccttgcct ttcagatact gttgccacca ctttagctca
     2281 gccttttgtg cgatattcca ctttatcgtt cgtttcatgt ttacctttgc aacggcttca
     2341 aaggtaagta aatcatgcag atacctgttg gtgaacaagt ggttattctc gattcaggca
     2401 ttattggtgt tattgaatac tataatggca gcacagccta tatcaacgta aatggcgaaa
     2461 tccatcaggc acataaaaag gatattgcct cagtcaggga atttggcgga aagggaaaat
     2521 ctactatcaa aaaagaggag ctttcccagg ctgcaaaaga tataggttta ttcattcatt
     2581 ggcgtgctgt aaaaaacatg ggtggcgatg tcgacagatt tgaagttgaa cttgtcaata
     2641 acacaccctt gcgattgtta tttgagtata aatggtattt actcgaagaa ttgcaaacaa
     2701 ctgtcaggaa tacaataacc cccaatgcca ccatcaggct tcatggtatg aaacccgatg
     2761 ctatcaatga tcgtccggta tctactttgc aatgctggcg tcaggatgat aaaggaatta
     2821 caatctcagt tgcagatagt gaggtcaaac tacgccctaa gcagtatgtt accaaattat
     2881 caactgaacc gtacctttca tcaggtataa ttgttttccc ggttaatgtg tcagacagta
     2941 gtcttcaaat tcctgctagc gaaaagaaac caacacctgc atttaatttc aacgaaaaga
     3001 aaaaagtccc ggtaataaac gaggtattac agaaggcagc aatgccggac tatattgatc
     3061 tgcatgtaga aaaacttttg ccatcacata agcatctgtc ctccactgaa attctggcta
     3121 tgcagattag tcaattcagg cattttctag aaaaggctat tcgtttgaaa ttgcatcgca
     3181 tttatgccat ccatggtcat gggaaaggtg tattgcgcac cgaaatagag aaaacattga
     3241 agaaatatcc tgaagtgaca tcatacaata acgactataa cccgcgtttt ggtcatggag
     3301 ctacagaaat tattttagac tagtctaaat ttttagatgg cgtcaaatcg gcaaaatccc
     3361 taattttgag atgagttcaa ataatcgttc ttaaagtaat tctcgcaaac cgttctatcg
     3421 ctgcttaacc ggtgcatgca tcggggtagt ggaggaaagt ccggataaca cagagcaccg
     3481 caccacctaa cagatggata tcggcttcgg tcggtaatgg atagtgccac agaaaattac
     3541 cgtcctgaca aaagtgttcg cacttttcgt cagggtaagg gtgaaaacgc gaggtaagag
     3601 ctcacgggtg agaatggcaa catttttgcc gggtaaaccc tgcgggttac aatgccaaat
     3661 atgccggagt aaaagaagag ttgctcgctc taatcttcgg tgggtaggca gcaagatcct
     3721 tatagtgatg taaggaccag ataaatgata gaaactcaaa cagaatccgg cttatgagtt
     3781 tgcgggaatt gttttaagaa tgaaacaggt atggaaacgt tgaaaaatgt tattctttgc
     3841 ccattttgcg gacacctcag ccaaatgctg tgggtgcatg gtcatggaca gtgtgccatt
     3901 tgcggaatga ccatcgatga atgttgcaga ggtgaacaag cttgtgaaat tgaagaccct
     3961 caaaagagaa aagcaaagaa aaaaatcact cctcaaaaag aagaacctaa taatgaaact
     4021 actaaacctt aagtctgcat tgttgatgtt agcttgctgc ctgggaatac aatttgcctc
     4081 ggcacaaaat cttgtcacgt tcgcaccgcc cgaacctcct aagcctgcag tgcctgtaaa
     4141 tatcgataca gttattacta ccatcgatgc agcaaaaggg tataaacgca ttaccatcac
     4201 cgcgaataat attgtggtat ctgatggcta tatgattggc aataaaaaaa ccggaacatg
     4261 gtccacattt caattaaatg gctctttgct taccttggcg gaatatgata atgacgtaaa
     4321 atccggtaac tatcttgaat ttgataaaaa tggtaaccta cttgtactcg aaggatataa
     4381 aaatggtaaa ctgcatggcg accaaaaaaa atatacacag ggaaaaacag gaagagtgct
     4441 gaaaagcagt tatggatacg ctgatggatt ataccatggc gattgcatag aatatacagt
     4501 tgacggtctg atacaatcgt ctatgcatta tgctaatggc aaaaaagatg gcacttctcg
     4561 ttggtatttt tcctccggaa aagttgccat ggaacaacat tatacaaacg accagctaaa
     4621 cggaaaacaa ctcatttacg atcaaaccgg ggccttgatc tctgaaggca cctataccaa
     4681 caatatgaag tccggaacat ggaatgaaaa ctatcccaac ggcaaactca aatcgaccgg
     4741 ccaatatacc aacgataaga aaaccggcga atggaaatac tacaaggaag acggtagtct
     4801 ggagaagaca gaaaaattct aaaaaatagt acaaacatta tatatttcct gtccaatttt
     4861 ctacatttgg gcacgtttac ttttttggac aaggttcaca cacttctttg gacaatcaag
     4921 taaattgaac ttaacacgaa ccaacacaat gaattaacga acatggctag tttattatta
     4981 attgacgacg aaaagagtat caggaaaacc ttaaaggaga tactcgaata cgaagggtat
     5041 acagtagatg aagcaggcga tggtgccgaa ggtctgcgta tgatacagga tgctgattat
     5101 gatatcgttc tctgcgatat caaaatgccc aaaatggatg gtatggaggt gctcgaaaaa
     5161 gcacaaataa caaatccgga tgtgccattt attatgatca gcggtcacgg aacactggaa
     5221 acagcagtgg aagcagtgaa aaaaggtgcg tttgattatg tagcaaaacc acctgatctc
     5281 aaccgactac tcatcacaat ccgcaatgcc ctcgataagt cgaatttgat caccgaaacc
     5341 aaggtgctca aacgcaaggt gagcaagact cgcgaaatta tcggcgattc tcctgccatc
     5401 atgaaaatta aggaaaccat cgatcgcgta gctccaactg atgcacgcgt acttattacg
     5461 ggtgataacg gtaccggcaa agaattagtg gcacgttgga ttcatgaacg aagtaaccgt
     5521 gcatcaggtc ctttagtgga agtgaactgc gccgcaattc cgggcgaatt gattgaaagc
     5581 gaattgttcg gccacgaaaa aggggccttc acctcagcaa ttaaacaacg cataggtaaa
     5641 tttgaacaag caaacggcgg aacattattc ctcgatgaaa ttggcgatat gagccataat
     5701 gcccaagcga aagtattgcg tgcattgcag gaaaataaaa ttacacgcgt tggtggcgat
     5761 aaagaaatat ctgtagatgt acgcgttgtt gccgcaacca ataaagatct gcttaaagaa
     5821 gtggaaagca ataatttccg cctcgacctc tatcatcgat tgagtgtaat tctcattcat
     5881 gtgccttcgc ttaacgatag aaaggatgac atccctctgc tggcagagcg cttcgtaaaa
     5941 gaaatctgtg aagattatgg tgtgcccaaa aaagtagtta caccaaaggc aatgatagaa
     6001 attcaaaata tcaacttcac aggtaatatc cgcgaactcc gaaatgtgat agagcgattg
     6061 gtgattttat ccgatcaaaa aattacagac gaagatgtgc tgaattatgc ccaatcacca
     6121 aaaagtggtg gcagcccaaa agacctttac gaacgcttcg aaaaatttca ggattttaaa
     6181 gattttgttg aaaaggaatt tatcctgcat cgcttgaata aaaacaactg gaatgtttcc
     6241 aaaacagccg aagagttgga tattcaacgc agccacctct acaataaaat cgaaaaatat
     6301 aatctcaaac gtgattgatc ttttttctca tggcagcgaa taaacccaaa gctcaaagca
     6361 aaactgatca aatgccaaag gataaaccca tggcaaacga atctcaggaa aacattgtca
     6421 ccaacgctgc caatatgctt attggtattg gcagcgtggt gatcatggcg cttataataa
     6481 tttggatgat caagctgcta ggctgaacat ctttttctgc tccgtaaaat cctgatgttg
     6541 ccttgtgcac ctgaaatgtg catgggtatt aattaactgc aaaaacaatt catcaaaact
     6601 caaggagtac ctgtaactct ttccagttct actgtttttc cttcaatggc aagtatggta
     6661 tttgaaaata cttcctgcgc ctcccgaagc aatggaagca aatcgtcata cttggaagag
     6721 aaatgtccta tcatcaattt ttttgcattc gcttgtaacg caattgaagc cgcttgtttt
     6781 gttgtagaat gaaaagtatc tgttgcacgt tgcgaacttg cttcatcaaa tgtgcaatcg
     6841 tgataaacta aatcggcatt tgcaatgaaa ggaaccatcg ccggataata ggctgtatcg
     6901 cagcagtaag cgtaagttct tggaagagga gggtctatag taatgtcttt attcaggatg
     6961 cgattacctg ttttctcatc caatatgtcc attccttttt tgatatcagg tatcaattca
     7021 acagggatct gaaattcttg taatttttcc ctaatcacct ttcgatcgcg ctgtttttcc
     7081 cgaaatacaa atccaacaca aggaatgcga tgattcagca caatggtatc taccgtaata
     7141 tcctcatttt cataaatcgt tccggtattt acagtatcca attcatgaaa tcgaagttca
     7201 taattaagct gactttcacc cgcctcaagt tgcattttga tgatgtcggc aagcggtgca
     7261 ggtgcataaa catcaagtgg tcgatttctg cgcatcaaat gataggtcga caaaacaccg
     7321 ataagtccga agaaatgatc gccatggagg tgcgaaatga aaatctgatt gatcttcatc
     7381 cacttgatac ccagctcatt aaagcgcatc tgggtgccct cgccacagtc gattagatat
     7441 aaacggtcgt tgatggtcac cacctgagca cttggatgcc tgccatgagc aggaatagca
     7501 gaattactcc caattactgt gaccgcgaat gacataccct tattcgttgc ctaactctcg
     7561 ctcaatctct tccataagga tgaaatcggt agcctcggat tcggtaggta caatgttcaa
     7621 tatactgtca agctgcgaaa tcttcaccaa cttgatagtt gactcgccac agccaataag
     7681 cacaaaggtg ccattgctgt ccttacagat gcgatgccct atcagcaagg cgctcaagcc
     7741 ggaactgtcg atgaaattca cctcgctgag gtcacatata atgttgcgga atccttcctg
     7801 attcagcaaa accagctcac ttttcaaatt tgcagataag atcgaatcca aacgcggctc
     7861 atccagctta aatatgcagt aacgctcctt tttgtctact gtaaacttca tacgctcagt
     7921 tgtggtgtaa aaataaactt taccccgcga aggtaggtaa ttaaaatttt ttccaaattt
     7981 gttagtctaa gtgaaagggc cctatggaat tttttaggct cctgaatgtt tacatagata
     8041 agacattgtc gaaataacaa tttcggcccc gaatttgtta atttgtcagg taacgcagag
     8101 ttggtaaaat tcttgaacaa taatcagaat ggacgcaaaa ttttctccta aagtaaaaga
     8161 agtgatctcc tacagccgcg aggaagccct ccgcctgggt cacgaccaca tcggaaccga
     8221 gcacctcctc ctcggcctta ttcgcgatgg cgacgggctg gcgatcaaag tgctgctcag
     8281 tctcgacgtg gatattgcca tgcttagaaa gaccgtagag gacgctatac gcgataaagc
     8341 ctctcgtcag cctttcaacg ccggcagcct tcccctcact aaacaagccg aaaaagtgct
     8401 caaaataacc gtccttgagg ctaaagtcct taagtccgat accataggca ccgaacacct
     8461 tatgctgtct atcctcaaaa acaaggataa tgtggcaaca cagattttag gacaatttga
     8521 cgtggactat gacgtatttc gtcaggaatt ggattatgtg cgccaggatc agggtcaacg
     8581 tgaaattcct ccaacaaacc aaaatgaaga tgaaaacttc gaagaggagg aaaagggcga
     8641 ccgtggattc aatgttccac gcaaaccggg tgcggcgaaa tcgaaaacgc ctgtgcttga
     8701 caatttcggt cgcgatgtaa ctcgcgcagc tgaagaaggt cgtctggatc ctattgtcgg
     8761 tcgccaggaa gaaattgaac gcgtttcaca aattctcagc cgcaggaaga aaaataaccc
     8821 aatccttatc ggagaaccgg gtgttggtaa aacagcaatt gttgaaggtc tcgcactgcg
     8881 cattattcaa cgcaaagtgt caagggttct gttcgacaag cgtatcatta tgctcgacct
     8941 cgctgcgttg gtggcaggaa caaaataccg cggacaattc gaggaacgca tgaaagcgat
     9001 catgaatgaa ctcgaaaaaa atcgcgacgt aattctcttc atcgacgaga ttcatactat
     9061 cgttggtgca ggcggtgcaa cagggtctct tgatgcatcc aatatcttta aaccggccct
     9121 cgccagaggt gaactccaat gtattggtgc ttctaccctc gacgagtatc gtcaatacat
     9181 agaaaaagat ggcgccctcg atcgccgctt ccaaaaagta ttggttgatc cgccaactgc
     9241 tgaagaaaca atggaaattc ttggcaatat caaatcaaaa tatgaagaat tccataatgt
     9301 gaattattca gatgctgcca ttaaagcctg tgtaacactc agtacacgtt atatcactga
     9361 cagatatcta ccggacaaag ctatcgatgt actggatgaa gttggtgcac gtgttcacct
     9421 caaaaatatt catgttcccg atgagatcgt gaaactggaa ggtgaaattg aagcaataaa
     9481 agatgaaaaa aataaggttg ttaaaagtca gcgttatgag gaagcagcaa aattgcgcga
     9541 caccgaaaaa cgcttactag aagacctcga tcgggcaaaa acgaaatggg aagagcacgt
     9601 aaaaagtgaa cgctatcctg ttactgaaga cgatatcgct gaagtggttg ccatgatgac
     9661 cggaattcct accaaacgca tcgcgcaaag cgaaggcact aaactggttg gtatggctga
     9721 tgagctgaaa cgcggtgtta tcggacaaga tgatgcaatc gtaaaaatca cacgcgctat
     9781 tcaacgcaat agagtgggac tgaaagatcc taaaaaacca attggttcat tcattttcct
     9841 cggacctact ggtgttggta aaacagaatt agcgaaagtg cttagtgaat atttattcga
     9901 ctctaaagat gcactcatcc gcatcgatat gagtgaatac atggagaaat tcagcgtaag
     9961 ccgattaata ggcgcgcctc cgggatatgt tggatatgaa gaaggtggtc agcttactga
    10021 aaaagttcgt cgcaaaccat attctgtaat cttgctcgat gaaatagaaa aagcgcatcc
    10081 ggatattttt aatattttat tgcaagtact agatgatggt attcttaccg atggattggg
    10141 aagaaaagtg gatttcaaaa atacactcat catcatgact tcgaatatcg gggcacgtca
    10201 gctaaaagat tttggtacag gtgttggatt ttctactgca gcaaaacaat tggcgcagga
    10261 agaaaatacc aagagtgtaa ttgaaacggc cttaaagcgc acattctctc ctgagtttct
    10321 aaacaggatt gatgatgtgg tgatattcaa ttcactcagc cgtgaagaca tcttccgaat
    10381 tattgatatc agcttgcgcg atgtaactaa acgtcttgaa acgctgaatt ataaattgca
    10441 attggaagaa agtgcaaaag aattcttgag cgaacgcgga tatgaccctc agtttggcgc
    10501 aagacctttg catcgcgcaa ttcagaaata tctcgaagac ccactggcag aaagtatcct
    10561 tggcggaaaa ttcaaggaag gcgataccct gctcgtggaa tttgataaag atttgggcgg
    10621 attagtgatc aaccctgccg gcgaaaagga agctgaaaaa gaagctgaga aggaaaagaa
    10681 aaaaggcaaa ggcaaggata aaaaagagga gagggaagag gaaaaagcct aatcttcaac
    10741 atacttaatc aaaggccgta tcgagagatg cggtcttttt tttgtcaatt ccttgtggcc
    10801 ttattttaca tggttgtgat agaaatattg tgataaaact gagcaataac gtatatttga
    10861 atatatgtat tccccgcaaa tgaaattcgc aatactgtat gctttaatgt tgatgtcaac
    10921 tattgcctca gcacaggcag actttgaatg gtatgcttct tataataatg ttaccttttc
    10981 ttctttcggg tatccaaagg atattgtatt agacaatgaa ggcaatgttg ttgcctgtgg
    11041 gtatactgct atcaatgata cttcacaaag tttgatgact attattaagt tatcgcctgc
    11101 aggtgatcaa ttatggaatt tcaatatgac cttgagtgac gaataccccg cactggtaac
    11161 agcactcgat gtcaatagca ataatgaaat tctgtttacc ggcgaaaccg gagataccat
    11221 gatcgtcggg aaacttaacc ctgatggctt attgcaatgg attgaaaagt atgccattaa
    11281 caaatactgg aatcatggta gagatattca tgtaactgca acagacgaaa ttcttgttag
    11341 cggcgattgc tctaacgaca cggttgtgct gctgaaatat acaaatgacg gcgatctgct
    11401 atgggaacgt ttcaggcaaa ggcaattcta ttttggattc gtatatacgg catttgacag
    11461 cgagtcaaat acttaccagt gctacgaagt gcccggaact gatgaatata cagatttcga
    11521 agtctgtaaa tataatgcgg aaggcgattt aatgtgggcg catgcctatt ctaatttctc
    11581 gcttaccgca tttgccagag attttgccat cagtgacgac gggtgtgctt atgtgctcaa
    11641 tagagcctat ggaaattcgc agttatatga gttagtaaaa attgattcgg ttggcgatct
    11701 cgcttggtca gcatatctcg ataatttccc ggatcaaaat tactttacct atgcaactga
    11761 tattgctgta gacaaggata aaaatgtaat ggtaatcggc gagcaatatg acagtgattt
    11821 gttagtggat tataaaaata tcgtaaaatt caacgatgac ggcgcttatg tttggcaaaa
    11881 gggggttgat tatatcgcaa atacagttag ttatggtagc gatattttca cagataacta
    11941 cggaaacatt tattcaatgt ttaattatgg tgataccatc ccatggtcgt caacctatat
    12001 gatgataaac aaacgcggtc cttccggaga tctgaaatgg agtgctacat tcagtgaaaa
    12061 ctgttatcct gagcaactgt tggtgaatga ggatgaagtg tatgccctca tcaccagtcc
    12121 cggcgacaat ttatacgatg ctattggtgt gattaaatat tcgcaagtgg taaccatgca
    12181 atcgcaagcg ccgcaaacaa ttgccatgca ggcataccca aatcctgcta ttgattacat
    12241 tcaatttatg gcaaatgcga atgttgaatt aatgcatgct gaaacctaca atatagcagg
    12301 ggagcttgtt gatgtaaata ttaaattgaa tacgccttgc gatgtgggaa tgttgcctgc
    12361 cggatattat acttcaagat gtattacatc actcggcaca gcgaccatag cctgggttaa
    12421 acaataagca gatgtaaagt agtttcgcga atggcttatt tcaccaattc attggattta
    12481 atcttcaccc tgtttttttt cgccttgcgt ttttcataag ccaacaccct gtatttatcc
    12541 gacaacatta ataaagcagc ttgggtatct tccttggttt gcggtgtgtc ggaaatactg
    12601 taactgcctt tttgcgatat accggtgagt ttattgtgtt gaaaaatata acgacctgcg
    12661 tatgaagttt taatggtatc cggatttatt ttaggtgttg cctcggcgtt gtataatttt
    12721 tgtgtttcat tcaccagcga aagagtatca tcccaataat aataatctct ggtaacttca
    12781 ccgaaattaa atccaaacca agtttcaatt ttgtaaatgg tgtcgttttt gaaataggcc
    12841 tttaacgttg cgccttgctc cggcatcgca tttacaaagg tggtatcgtc gatcaatata
    12901 taataatgct tgcgcagctt ttgagtttgt tgagcggaag cagaaaatac caggatgctt
    12961 gacagcagta aaaagaggag ttttcggttc ataatagtgc aaacaaagtt aaatcaataa
    13021 ttgttacgaa gatgtaaatt tgtccgatgg ctggcctcag agagaaactt ctggctcccg
    13081 acaatagggt gcggcgcaat cctatggtca tagtagtgaa tatgcttttg gccagtattg
    13141 ttgtgctgtt tctggcactt acctacggct atgcccttag aatgggtagt aattggctgg
    13201 aattccgact tcccaaagtg ttttggctta gcacaatttg cattatgttg gtaagctttt
    13261 ttcttagacg gacaatcaaa tattacgaca ccgatcgcac ggcccgcctg cgcagagcaa
    13321 tggcattggc aaccattatg gcaacagctt tcgtggtatg tcaactcata ggttggggtg
    13381 aactgggaaa agccggtatt ctgatggata cttccacctc tgcttcttat ttgtatttgc
    13441 tctccggttt gcatcttttg catgttggcg tggggataat aatcctgttg gttggttgtg
    13501 tgcgcttatt cacaaataca tctacgcctg taaaagccct tctctattat gcagatccag
    13561 tgcgacgcga tagattgaaa ttattaaatc attattggca taccatcgat ttcctttggt
    13621 tattcttgtt tttggtgata ctttataatc aaacataaac gccttgaaat aaaaagcccc
    13681 cttaccctga gaggatagaa gggggcttgt ttcactcctt aaccaactat attactcgtt
    13741 ctttttcttg tcgtgcttgc gttgcaattt ggcttttgct tcctctttgc tgtcagcatg
    13801 cgccttgatc tcgtctttat gcgcttttac agcttctttt ttgtcagcct ctgttttgtt
    13861 tgcttttgca tcttgagcat gcgcttttac ttcagctttt gaagcatcat gtgcctgaat
    13921 ctcatcctga tgtgccttta cacgagcttt tgcaggtttt gcattcattt tgccattcat
    13981 ggcagctttc tttttagctt gagcaggtgt gatagcctga cgcttttcag gagcaacttt
    14041 cttcacctca gttttcttag cctccttatt ggcatctccg ctcggagcac tctgagcaaa
    14101 cacttgtccg gcagttacag ccagtacaaa agataaggtc atcaatagct ttttcatatg
    14161 cattagtttt tagtttaatt gctaatatgt gatacattcc caaaaccgtg ccaacacctt
    14221 cttaatgatg ttaaggcttg ttatgaattc cccggaaagc ctgaaaataa ggtttttacg
    14281 gcactttgac attctcaatc gccatcccta cctttgccca cggtcgatgc gggatgtagc
    14341 tcagcccggt agagcacacg tctggggggc gtggggtcgc aagttcgaat cttgtcatcc
    14401 cgaccattgg aaagctgcct gatggtggct ttttacattt tatatagcat cagctcaatc
    14461 ggatttttaa cactttttat cattgttgaa ttttacctct gtttatgtgg tattcttcta
    14521 cgcaaaaggt catgctttat taatcgtgca attattcatc gcgttatact aattttaccc
    14581 ccggcagagt ttatcatcaa tatcgccatt catgcaattc gtttaccccg catttctgtt
    14641 tgcactcgca acactggcaa taccggtggt gatacacctg tttaattttc gtcgatttaa
    14701 aacaatttat ttttccaatg ttcgattcct aagaaatgta caggaagaaa cagcaacgaa
    14761 aaacaaactc aaacatctac tggttctctt aacgcgttgt ctcgctttag cagctcttgt
    14821 atttgcattt gctcaacctt ttattcccgc aaaaaaatcc ggtaataatt catctgatcg
    14881 cgcagtaagt atctatatcg ataactcatt tagcatggaa gcagaagtta acgatgagcg
    14941 cctgattgac atcgctaaac gaaaagcaga agaaattgtg aacggattta ctatcgatga
    15001 tagatttcaa ctcattacca acgaccttga agcaaaacat caacgattgg tcagcaagga
    15061 tgaaatgatt cgttttatcg cagaagtgca accatctcct gttgtaagac cacttcaaga
    15121 aataagtgat agacaacgcg atgcattaca ccgggaagga gacaaagctg aaaaaatatc
    15181 atacctgcta agcgactttc aaaaaaatgc aggcgacatt gataacgata ctagcatcat
    15241 cgtgaatttt gttccactgt tgcagcgtgc acaaaaaaat gtagctatcg attccatgtg
    15301 gttcaccact ccggtgcctg ttttaaatca accgataagc atttgcattc gcctgcataa
    15361 ttatggtgat ggcgatgttg aaaatgttcc ggttaccctg cgtttgaatg aacaaattaa
    15421 agctattgca gatataaata tcaacgctaa aggcgatgtt gttgatacaa tgaccttcac
    15481 acttaatagt gggggatggt acagcggaga gatcagtatc aaagattatc cggtcacctt
    15541 tgatgatatg ttgtatttca gcttcaaacc tgttgaacaa attccggtgc tggccattaa
    15601 tggtggaagc gaaaatattt tcctcaaagg tttttttggg aataataatt tgttccaatt
    15661 aaaaaataat ccggcaagcc aaattgcatt cagcgaactg cccacttatg atttgattat
    15721 attaaatgaa gtgccttcca tctctggcgg tctggcagat gccttgcagc agcatttggc
    15781 tcgtggtgga aatattatgg tatggccttc cttgacaatg gatgtagctt cttatcaagc
    15841 atttttattg gcaaataatg ccgctgtgta tggggcatta ataaatagac ctcgccctgt
    15901 aagtgaagtg gataccaaaa atccggtgtt tgcagatgtg tttgaaaaaa ttccacgcaa
    15961 cctggcaatg cccacggcaa tgaaaagcta tgcactgcag acaagcaccg gaacaagaga
    16021 agaaatgtta cttcggttca ccgaccgtca ggcaatgatg gcgcgctatg cctcaggtga
    16081 tggctatatt tatttatctg cagttccgct cgatcgcaca gttactgatc tgccggtgca
    16141 ggcaggatta tttgcaccga ttatgtataa agttgctgtc agcaatgccc gccgtctccc
    16201 ggcttatgcc aatatcggtg aaatgggatg gataaacctt cccggaattc aattacccgg
    16261 cgatgctacc attaaagttg aaagcaaagg aaacgaattc atcccggaga ttcgcagaac
    16321 aggcaatagc gtagcggtga atctcagcag ctatgtgcaa caggccggat ggtatactat
    16381 cgtaccggat aagcagatgg taaacagcga tcaattgttg gcgttaaatt ataatcgaag
    16441 cgaatctgac atgtcatttg ctgatgcatc ggcgctgaag gagttgtatg cagcctcaaa
    16501 tcaggagatt atttcagata tcgaccgcaa ttttacggga atggtaacgc aaatggccac
    16561 cggaacgcct ctctggaaat attgtattat atttgtcctg gttttcctcg cggcagagat
    16621 tgcaattatt cgcctgatgc cgtagtaaac atccgttatg cactgcattc ttcagggagc
    16681 caccatcatc gaccccgctt cacagcatca tcgcagcact gtttccattc gcatacagaa
    16741 tggcgtaatt accgaaattg cctcatcaat tgctgatacc ggagacgcgc agttgatcga
    16801 tctcaaaggg aattttgttt ccccgggttg gatggatatg ctcgcagctt ttggcgatcc
    16861 gggacatgaa tatcgggaaa caatacaaac aggtgccgct gccgctgcaa aagggggatt
    16921 tactgccctc ggacttatgc cgaatacaca acctgcctta catggtaaag cagagatcga
    16981 atacataaaa aatcatgcag ccggtcagct cacacatata tttccctatg gtgccgtaac
    17041 gcataataga gaagggaaag accttacaga aatatatgac atgcatgctg caggtgctat
    17101 ggcatttacc gatgcagatt cagcaattcg tgatgcggga attttattgc gcagcttgca
    17161 atatgtaaag ccgttcaacg gagtgattat caatatccca aacgaccata aaattgttgg
    17221 caacgcaaat gtgaatgaag gtgcaatgag tgttcaatta gggatgtatg gtattcctga
    17281 tatcctcgaa gaactcatgg ttattcgcga tattaaactt gcagaatata ctcaatcgca
    17341 tttgcatatc ggtatagttt catcagcaaa agtgttacca catattcgtg cagcgaaaga
    17401 aaaaggactt tcggtaacgg caggagttgc tgcttatcag ttgtattttg atgaaacgga
    17461 attgcacgat tataatgcgc attataaagt aaatcctccg ttgcgtacag ctgccgataa
    17521 acaagccttg cgcgaagctg ttgcagatgg cactatcgat gtggtgtgca gctaccattt
    17581 acctcatgat aatgattcga aagatgttga attcgaatat gcagcatttg gaatggcttc
    17641 attagaagtt gtattcggcg ccatgaggtc agcacttcca aatataagtc cggaacaaat
    17701 cgtaaatatt ctggccgcta agcctagaaa tattctcaat attgcacaac cgggaattgc
    17761 ggttggaaat acagccgaca tcaccatgtt tgatgcggaa actacctgga cacttcaaca
    17821 aagtgacttg cgatcaaaag cttcaaataa tccttttgtt ggtaaaacat tgaaaggaaa
    17881 agttttaggt gtcattaaca aagggaaaat ctcaattgca taggatggaa gggcaggata
    17941 aaataatgga cgtgcaacca attgaacgcc caagtcgcaa gggtcttata ttgcgaaacg
    18001 ggttaatctt tgttgcatta caaattgcaa ttatgttggt aacccatttt cgccgtgata
    18061 gcattagtga tcaaaacatg cgtattgcgg tgggaatgta cctgttattg ttagcagcta
    18121 ttgttgtggt gcaacttgat gcacgtatga gggtttatag cggtagaatg gattttggac
    18181 aggcattttc tacaggaatg atattcgttt tgtttgtaac agcattattt acgatattta
    18241 ttcttgtgtt ttatctaact ataggaaaag agttgcttgc tcaaatgatt acaattgctg
    18301 aaaactcatt gcgggcaaat ggaacagcag aagcagatat tcccgagcga atggaatttt
    18361 caaggaaaat atttaccgct ccgggaatga gcatcattac ccttataggt tatttgtttt
    18421 ttggaacaat aatgagttta atcggtgcac tgttcactca aagaaccaat taatgcaaat
    18481 cagtgtaatc ataccactac tcaacgaaga ggaaagcctg cctgagctgt cgcaatggat
    18541 aagcaaggtg atgcaggaaa ataatttctc ctgggaagtg atatttgttg atgatggcag
    18601 cagagataaa agctggcagg tagtgcaaca attacatgcc gataatgcct ccttcaaggg
    18661 cattaaattc cgacgtaatt atggtaaatc cgcagcttta aacgaaggct ttgccatggc
    18721 gcaaggcgat gttgttatta caatggatgc cgatttgcag gacagtcccg atgaaatccc
    18781 cggattgtat aaaatgattg cagatgaagg gtttgatatg gtaagtggat ggaaaaagaa
    18841 acgtcatgac cctattggaa aaactattcc aagcaaattt ttcaattgga caacggctcg
    18901 catcaccggc attaaattgc acgattttaa ttgtggcttg aaagcttata aacgcgatgt
    18961 agtgaaaagt attgaagtgt atggtgagat gcatcgatat ataccccaga ttgctaaaac
    19021 tgccggattc tataaaatag gggagaaggt ggttgaacac cgtgcaagaa aatatggtta
    19081 ttcaaagttt ggttgggagc gtttcatgaa aggatttctc gatcttactt ccatcatgtt
    19141 tgtgagtaag ttcgggaaaa aacccatgca cctcttcggc actttcgggt tcctgtcgtt
    19201 ttttaccggt gggatcatta ccatatggct tatcatagat aagatcatta aaatgaatac
    19261 cggcgaaaaa ttccgacagg ttactgatca gcctttgttt tatatcgcat tagttgccat
    19321 gatagttggt gtgcaacttt ttgttggcgg atttttaggt gaactcattt caagaaattc
    19381 tcccgaccgc aatcaatacc agatcgatca acgaatcgga ggcgtatgaa aatattgctc
    19441 ataggcacag cttggccata tcgtggcgga ctggcctctt tcaatgagcg cctgattcag
    19501 gaatttatta ccgaaggtga tgatgcttca ataataacct tcacattgca atatccggga
    19561 tttctatttc cgggaaaaac gcaatatagt agtagtcctc cacccgggat acctatcacc
    19621 agaaaggtga atgccgtgaa tccatttaat tggtggcgaa ttggtaaggc aattgcgaaa
    19681 gaaatgcctg atattgttat cataaaatac tggatgccat ttatggcccc gtgctttggt
    19741 gccattgcaa ggcaaatcaa aaagaataag catagttcca ttatttgtat tgcggataac
    19801 atcatcccgc atgagcgcaa attttatgat aagccattca caaaatattt tatttctgca
    19861 atggatgggt ttgtcctcat gagcaaaagc gtgatggaag acctcgcaca attcgacagg
    19921 gaaaaaatac gtgcgctaaa cccgcatccg ctgtttgata attttggcga aggcgtttct
    19981 aaaacggaag ctttagctca cttacatctt gaggcaaatc gcagatacat tttatttttc
    20041 gggtttatcc gcgactataa aggactggat attcttttgg aggcgatgca tgccattcct
    20101 gaagatgtac atctgattat tgccggtgaa tattacaccg atagtaaacc atacgaggca
    20161 ttaataaatc agaacaatct tggagaccgt atacatgcat ttaacaggtt tattccggat
    20221 gaagaagtga aatatttttt ctgtgcagcc gacctggtag ttcagccgta taaacatgcc
    20281 acacaaagtg gtgttacgca gatagcctat cactttgaag tgcctatggt ggtaacaaat
    20341 accgggggac ttgcagaaat ggttttggat ggacaagtag gatatgtcac tccggcagag
    20401 ccacttgcta tagcacaagc agtgtcaaag ttttatgtgg aaggaaagtc ggaggaattc
    20461 aaaaggaacc ttcgagaaga aaaaagccga ttttcatggt cgcgtatgac atctacgata
    20521 cgccggttgt accaaaaagg ttagtattat ttgcttactg ttgctttttg ttgctgaaga
    20581 aagtaatcag gaaaaataaa ggcaagccca tcaggaagat taaaaatcca tactgagcag
    20641 aagttgcatc atccataaat gtgctatagg tgacaaataa taacaccaac ataaaaactg
    20701 caggtatata cggataccct ttcatcttga aaccactata atcctttggt gagcggtgtc
    20761 tgaaaataaa caatgcacct gctgcactgg caagacttat gctatcaata aataaaacat
    20821 aggtaacaat gttttcaaaa ttgccaagca agaataccga tgcaatcatt actgccacaa
    20881 aaaatgataa accaaattct tgcacctgag ttttgtgatt aaccttgctg aatatttgcg
    20941 gcaatacttt atcttctgcc attgcatagt aaacacgtgg gttactcatc attgaggtgt
    21001 ttagaaatcc caatacagaa gtaaaaagta ttatggaggt gatcacagcg ccggtgttcc
    21061 caaatagtgc ctcagccaga tctttggcaa tcaatggcgt atttttcatt tgttcaaaac
    21121 caagaacatt cacataggcg aggtttatca gcaaatacaa tacaataaca atgcctacac
    21181 caaagaaaat ggcaagggga atattttttt gtggatcctt cacatctgca ccaaaattca
    21241 ctgtttgttg atagccgcca taagtaaaaa atatagatat caatgcaatc ccaaaggcat
    21301 ttatgaaatt ccagtcctca tcgatgacaa caggaatatc agcatttgcc tgtcctgaaa
    21361 agatcgcgaa gcaacaagta ataatcatga caatttttag aaggctgagt acattctgtg
    21421 cacgagcgcc cattttaata cctgcataat tgattccaaa taaaataaga ataattacaa
    21481 tcgcagcggt gtcgatcaca acatagtctt cagctgtgtt accgaacaat accggcgtca
    21541 gatattcagc tccaatgatg gctacaccgg cactggaacc tgcattgata atcacctggc
    21601 tccagttcaa cataaaggca tatatgggat gataggcagt cgagaaaata cggtagtaac
    21661 cgccggcact cggtagacgc gccccgattt cagcaaaagt aagcgctccg aaaattgaaa
    21721 taatacctcc tgtaatccat gcagcataaa aaatccattc cttccctgca ctttgtgcaa
    21781 ccatggcagg tgttctgaaa attccaacac caattaccag gctcacaaca atcatggtga
    21841 ggtcgaatct tttgagttgg ggttgaatgg acatttgtca aatgtaaaga actgtaacca
    21901 tattaaaaac aaagagcctc ccgaaggaag ctctttgttt ttagattgta atgattatcc
    21961 taaactattg gaactatttc aatcacatat tcgcaataat ctcatcgccg aattcggagc
    22021 atttcaacat cgttgctcct tccatcaggc gatggaaatc gtaagtaaca cgttttttag
    22081 caatggcttt ttccattcct tgtatgataa ggtcatttgc ttctttccat cccatgtatt
    22141 caagcatcat acatccactt aaaattactg atcccggatt tactttatcc tgattagcat
    22201 attttggtgc tgtaccatgc gtagcttcaa aaatggcgtg tccgcttaca tagttaatgt
    22261 tcgcacctgg agcaataccg ataccaccaa cttgagcggc gagtgcatcg ctgagataat
    22321 cgccattgag gtttaatgtg gctattacat caaactcatc aggacgagta agtacttgtt
    22381 gcaaagtaat atctgcaata gcatctttaa ttacaatacc tgcaccgggt tttccttcag
    22441 ggattacatg ccatggacca ccatccagtg gaacagcgcc aaagaaatcg cgtgcaacct
    22501 gataacccca atcgcggaag gcaccctccg tgaatttcat gatattgcct ttgtgcacga
    22561 tagtaaggct cttgcgattg ttttgaacag cataatcaag agcagaataa atcaaacgct
    22621 ctgaacctga atagctcaca ggtttaatac caatgcccac ctgaacatca acgtcgcggt
    22681 ttggaacacc tacggagcgg agtgcctctt cccaggcatc agtcttggct ttatttccaa
    22741 aacgaatctt ttcgaagtct ttaggtgaat tttcttgaag ccaattgagg aatttaccta
    22801 ccaaatcact tcctgctgcc caatcgatac cggcataaat atcttcagta ttctctctga
    22861 aaataaccat gtcaactttt tcagggctct tcacaggtga aggcacacct tcgaaataac
    22921 gaacaggacg aagacataca tacaagtcca ggatctgacg cagtgcaacg ttcaatgagc
    22981 ggataccacc acctaccggc gtagtcaagg gtccttttat gcctaccaga tattcacgga
    23041 aggcatccaa tgtttcatca ggaagccagt taccggtttg gttaaaagat ttctcacctg
    23101 caagtacttc catccaatga atcttgcgct taccaccata tgctttggca acagctgcat
    23161 cgaaaacgcg cactgaagca gcccagatat cggcaccggt gccatcgcct tcaataaatg
    23221 gaataatggg ttcatcgggc acattgagca ccccgttttg tatcgtaatt ttttgtccgc
    23281 tcatgataat atgttatttg aaatgttgcg caaagatacg attctgcggg cagagctgac
    23341 aggaatatcc tcaggttgtt cactttgtcg gtggtccgtt tggtgttaat tccctaatct
    23401 tacggtatga ataacaaacc gcagcttgta agatcactgg gactgagcac agccattttg
    23461 cttgttatca gctctatgat aggcagcgga atttttaaaa agattgctcc aatgagtgag
    23521 caactgcccg acagcaacct tattttgctt gcctggttgc ttgccggtgt cgtctcgatg
    23581 ctgggtgtat tcacctatgc cggattgagc tccatgacag aagagggtgg ggggcagttt
    23641 gaatattttc gaataattta tgggaaattt tttggttatc tgttcggatg gacatgcttt
    23701 gctgttatac aatccgcatc cattgcttct attgcctacg tatttagtca atcggcaaat
    23761 aatttattgc caatgggcga tccgcttgcc gcctgggccg atatatcaat agccggaaag
    23821 atttttccat ttagtaacag cggagtgaaa atatttacca tcattacaat tgctgtgctt
    23881 accttgatca attattatgg agtaaaaaaa ggcggactat taaatgatgt attttcagct
    23941 gcaaaaattg ccggtattgt atttttgatt attgccggac ttgcattgca tggcactgtt
    24001 actgaagatg catccattaa tgttccggag ctgcctcagg gattatcgct tttgtcgacc
    24061 atgttcgcag ccatgcttgc ggcattttgg gcttatgatg gatggataaa tatctcttac
    24121 atcggtggcg aagtaaaaaa tcccaaaaaa aatattccta ttgccatcat aggtggaaca
    24181 gcagtagtga tgttgttgta taccttggtg aattatgtat atctgcatgt attaccggta
    24241 tcgtcattta ttgcgataaa aaattcagat aatcaaatag cagccgcaga agttgcacag
    24301 catttacttc cgggttttgg atttacgttg atctcaatat taattatgat ttgcaccttc
    24361 ggagcaacaa atgcaagtct gatgtcatcg ccacgtatct atcatcaaat ggcgaagaaa
    24421 aatttatttt tcaaaagctt cggcgaaaca catccaaaat accatacacc gcatatttcc
    24481 cttttttatc aaatgatatg gagcagcgta ttagttatat cgggcacttt tgatcagctt
    24541 accgatatgc tcatatttgc ttcatttatt gcctatggaa gtggcgcagc aggcttgttg
    24601 tatatgaagt atgttggcaa aaaaattcaa atgcctgatg gtactgtgca gcatcagcgc
    24661 ataacggcta aggtgattgg atttcctgtc attccaattg tatttctgct tttctgtatt
    24721 gcgctggtgg gaaacacttt atttacttat acatcggaga gcctcacagg gctggcattc
    24781 atcgccattg gtataccact gtattttgtt ttccaaaaaa gtaaaaaccg gtcaatagac
    24841 ccaatcgata cgttggaata acaatttaca ttacaatttt aaattgcata aataaagaaa
    24901 ggggcaccga ggtacccctt ttctttagat gttaacttcc attacagcca tatggaagtg
    24961 ttaatctgtc agcacaacca gaaattttga agctttccag aattcatccg gattctcaat
    25021 tacgagttct tcaatagttt tatcgccttc aacaagtgta tatgaatctg aagtgtgtgt
    25081 agtaatcact ttcgctgatt tagaattcaa tgctaattct ttgaatttgc gaatgtccac
    25141 tttggtgaat gcacttgcat caaagttatt agtcagttct ttgttagcac ctattcctat
    25201 aaagccacct tctttgtcaa caataccgag ttcttttaat tctttaaaag ttccactgat
    25261 atagtatgca gtgtttaaaa catcagtttg atctgaaatc actttcgctt gttgcgtatt
    25321 ttgttcttca agcaattgtt ttgcagaagt aacagttgca aggtcagtat tcaaattagt
    25381 gatttggtat ttcgctgcct ctaattcagc acgcaaagcg gcaagatcct gctctttttg
    25441 ctcgagtgtt gcatttaaca gtgcaatgcg ctcgtcaagt tctttaatct tgagattatt
    25501 gcctttgttt ttgcgatcga ggtcttttac aagatttttg tttttctgaa tcagatcatt
    25561 caatgtagca atatttgcca tgatctcttc acgagtttgt tctttagagc gaccttcaga
    25621 atgcaaacta ttatctatca tgccttctgt tttcttgatc tctatcaagt tcgagtcgat
    25681 ctcgttcatc atgctcatgt aagaactaat ctcattcact gcagcatccc atccggattt
    25741 aaattcggca ctcttatttt ttagatctgc attttcctgc tccagcgcat ccaacttatc
    25801 ctggttacat gcgatcatca aaggagcgaa cgcgaatact atcaaatact ttttcatcgt
    25861 gttgtgattt ggttgatata tagaaaatat tttgccaagt tattcaaaat cgtcaaaacc
    25921 cttgaaaaac aagggttttc atttattaac gacaaaatta attcctttta tattgaggaa
    25981 ttgctcccca atatgtggaa ataaggcagt atgttttcaa taacctacac cttatctttg
    26041 aacttccgtg aagaatccca acctcgaacc taccgatgat tttatgagcg gttccgataa
    26101 ggaactcgaa aaagtattgc gccctcaggg attttatgaa tttacaggtc aggagcgaat
    26161 catcgaaaac ctcaaagtat ttattgccgc cgcaaagcaa cgtggggaag cattagacca
    26221 tgtgctgctt cacggccctc ctggattagg aaaaacaacg ctttctttta tcatagcaaa
    26281 tgaattgggt gtaaatatga agatcaccag tggtccggtt ttggaaaaac ccggcgatct
    26341 ggcaggatta cttaccagtc tggaagaacg cgatgtactg tttattgatg aaatacacag
    26401 gctgtcaacg gtagttgagg aatacctcta tcaggcgatg gaggatttta agattgatat
    26461 tatgattgaa tccggcccga atgccaggtc gatacaaatc aatttgaatc cattcacgct
    26521 tgtaggcgct accacaagat ccggcatgct tagcgctcct ttgcgttcaa gattcggtat
    26581 ctcttcacgc atagaatact attcggcaca aacactgcaa caaatagtta cacgctcatc
    26641 aggcatactc caaa
//
LOCUS       DAOQOU010000026        26538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_841_length_26538_cov_6.359665, whole genome shotgun sequence.
ACCESSION   DAOQOU010000026 DAOQOU010000000
VERSION     DAOQOU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26538
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_26538_cov_6.359665"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLM90_03930"
     CDS             complement(<1..810)
                     /locus_tag="PLM90_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HMZ88547.1"
                     /translation="MNSPNKEFHKSVEQQNDQSFNWRLFLTISWINRWWIVLVFAIGV
                     CAAFLYNRYSTPIYESHADIQFKDKSKDNTIDLGIRVANSNIDRMNEEMTVLSSKGYK
                     ITALSDLPIGVSYYIRERVKAAEIYNESPFTVNIAILDSAIIGDKIDVQILDTSEYKL
                     SYTYKNEKFSKKFSFNKAYSTPAFNINTTLNTFAKSKFKFEYFFTINDLLKESERIQE
                     DVSIDVENLYGGEISIKYRDKNRQKTQDVVNGIADEILKMSLHRKAESAKTV"
     gene            complement(814..1608)
                     /locus_tag="PLM90_03935"
     CDS             complement(814..1608)
                     /locus_tag="PLM90_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HMZ88548.1"
                     /translation="MTSFSKQPTKRPIIHLLLAGMLLATFSSCGMLYPNRMLDTGKDF
                     VESSFLDTIYREFVIEPGDQIQVFVYPNGGYNLIESQISNQMNYYQPQTMLQSLSYQI
                     DGSGMANLPRLGWMKISGLTESGAEKMLTTEYGKIYTDAFVNVVITGKYITVYRGGSD
                     AKQLALNRSDLTLLEAIGMAGGIPENGRSSKIKVIRNIQGAAQLQEIDLSSIDDIALG
                     NMYIQPNDIIYIEPNINSTVFQEIGPILSTISSIAVIYAFFANVNQ"
     gene            complement(1692..3617)
                     /locus_tag="PLM90_03940"
     CDS             complement(1692..3617)
                     /locus_tag="PLM90_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HMZ88549.1"
                     /translation="MERRWSNYINQNFGTRILSKWLVFCFDIVITIFTYGVAYILRYN
                     FNIAIISFSTFVDNTFLTTLVFTASYLAFKTYDGIIRHSGEAEARRIVMSGMLATIVC
                     LALSIIGRETGKPFLTLPASIAIIHTSINIAFLLFSRYVVKVLFYQATRNNAAAIPVI
                     IYGAGRRGLSVMHTLRRDTNHNYQITAFLDDNPSKFQKTIEGVRIYNSEKLPDLLRRF
                     VVKELIISIAHPIPDKKMAVVDACLQHQVFIKSVPPVDDWINNKLSTRQLRKFNIEDL
                     LGREQIHLENDKLLSAIGGKTILITGAAGSIGSELVRQLIPFQPKQLILVDQAESDLY
                     DLYMELQFRLNEKKIVTENIIADITNRKRLEDIFATYHPAFVFHAAAYKHVPLMELNA
                     IEATHVNVIGSMYLTDLAIQYEVEQFIMISTDKAVNPTSVMGATKRAAEIYVQERSQH
                     SRKTTFITTRFGNVLGSNGSVVNYFNKQIENGGPLTITHPDVTRYFMTIPEACQLVLE
                     AAVMGKSSEIYMFDMGDPVKITDLATKMVLLSGYTPGKDIQFTFTGLRQGEKLHEELL
                     HSNENTLPTYHQKIRIALTAPYPAEQVQHYLQMLQDAIVNSDIQQSVRLLKLLIPEYK
                     SNNSEFERLDKQDTAYK"
     gene            complement(3713..4807)
                     /locus_tag="PLM90_03945"
     CDS             complement(3713..4807)
                     /locus_tag="PLM90_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ88550.1"
                     /translation="MSGEEQRFVNDAFDSNWIAPLGAHVDGFEQELSAFTGSNDCAAL
                     SSGTAALHLALIMLSVGKGDIVICPTLTFSASANPIIYQGATPVFVDCDATSWNMDPN
                     LCESAIKELIAKGKKPKAIILVHLYGMPSDMDAFIRISHQYDIPLIEDAAESLGATYR
                     NQHTGTFGEFGILSFNGNKVITTSGGGALLCKTKEQANYARFLATQARDNAPHYQHSV
                     IGYNYRLSNICAAIGRGQMTVLSERVQRRREIFHYYYNHLKDIEGISFSTEMEQMKSS
                     RWLTTILINPDQTGTDREQIRLLLGASGIESRPIWKPMHLQPVFNDATTYNQGVAENI
                     FQYGLCLPSGSSLTAAQQQEIIDLIRSAVK"
     gene            complement(4856..5890)
                     /locus_tag="PLM90_03950"
     CDS             complement(4856..5890)
                     /locus_tag="PLM90_03950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88551.1"
                     /translation="MHITISPYNHISQITSLGLQPDDVYQIPSYCEMDALSIHAQPIL
                     ITATGAHGALALPLLLRQIPDAIAKGEKLFDAISPYGYAGILTNISDIGQLTEALNAC
                     RLEASKKNIVSIFIRMHPIHQKLIIPPTTSLNQVIHGNTVYINLENPVEYIKVSYSTG
                     LKRDLRKLMQLKIETKFNAWEYYDEFIEAYKETMALHQASNFYYFSRSYFESLRQPEF
                     NSILISSHTPDGTFISGALFFAHGNTVQYHLGGTKQSTRHLAGSKLIFDHAIDYFKLD
                     NRKAIHLGGGVGSAEDSLFRFKAGFSNERATFSTLRIICDEIKYQSLSADKISNMQNS
                     AKDFFPLYRL"
     gene            complement(5865..7010)
                     /locus_tag="PLM90_03955"
     CDS             complement(5865..7010)
                     /locus_tag="PLM90_03955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88552.1"
                     /translation="MYKHFIKRFLDIVFSLTAIAITLPFMVIIAVVCAITFKGQIFFI
                     QDRVGKNGAIFRLYKFITMRPQPAGKDLADTERLTAWGNFLRKSSLDELPQLFHILIG
                     DMSLIGPRPLLVRYIPYYTSAEWHRHDVKPGLTGLAQINGRNTLDWEARLNFDVVYAA
                     SYNFKMDLEIFIQTFIVVLKRSKYHIDPRTVVADFDIERKGNQIHISEGVILRPLYIS
                     DAGSLLEVKNNKEASVWLEKDPPVYTKEGLINWIRHHQSNPNNLILIIEDTINRKVIG
                     HAGLYDINPEDGTCIFGILIGLPEYWGKGIGRAATSSMIQIAFNTPGIQTIKLHVLQI
                     NERAISLYRSMGFTNEEVLVDQTIKNGEPQDVICMKMFNHAHNDISI"
     gene            complement(7016..8257)
                     /locus_tag="PLM90_03960"
     CDS             complement(7016..8257)
                     /locus_tag="PLM90_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ88553.1"
                     /translation="MHILLIHQYYQEKDDPGGMRWNAMTKLWAAEGHQITVIAGMTNY
                     ASGIKHPRYTNKYTYSEQFDTNIRVIRTHVSKSYNKSFRGRLNAYFSFVFSGIYGGLF
                     KARDKYDLILVSSPPLSVGIIALTLAFFKRRPFALEIRDLWPESAIDTGVLTNSTMIK
                     WSYRLEALLYRKALVTNVVTPAMQEVLINNKKVPANKVIYIPNAADFDMSDVLLLNFD
                     TAELRETLQLNGKLSLCYVGAHGVANHLEQLIIAAEKLRDLPVMFVFIGDGMQKQVLI
                     EMVTARNLKNVTFIGLITKSEALKYILACDIGLSVLKKADTFKTVYSNKTFDYMACKK
                     PVIMAIDGVSRKLIEDADAGIYVEPENTDEMIKAISTYLQHPELIKSHGENGYAYVKQ
                     HFDRKTLAQQYIRELTGRCSK"
     gene            complement(8397..10283)
                     /locus_tag="PLM90_03965"
     CDS             complement(8397..10283)
                     /locus_tag="PLM90_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HMZ88554.1"
                     /translation="MIRFIELYVDLFKGVGFKYWAYRFYHGLKKRTGILQYQFPIQAF
                     EPSDISKDFTIAPWGTFGNIHLDHTKIENNRETVTQRFTDVNNGHIQFFDGDVLKFTG
                     ENRWTTNAKTGKVYDNKIHWANLNDFASEEDIKYIWEPARFNYLYAVLQYDQCFGTDN
                     SACIWQEICSFIDHAEMNAGPHYMSSQEIAIRIINWTIFLDIYKHSSSLTTEVYQKIT
                     GSIHTQAIHIFKYLDFSKNLVRNNHVLSEAAGLYIIGTFFPQFPDSEKFRKVGWSLFE
                     KEINYQIAGDGSYLQYSMNYHRMVIRLFSLMLRVGKVNGHHFQQETIQKGLTTLEFVN
                     AFIDEKSGTVANYGNNDGTSLFPFTDTPYNDYRTCIATFSIALGANNPYPQLIEDAQW
                     FGFDIESGSKTEIPKQDLYHFNEGGYYIFNEQYSKTFIRCGDHPFRPSQADNLHIDII
                     YNGMNIMRDAGTYQYNSTPEVTAFFMGTKGHNTVSIDGLDQMLKGPRFVWHFWTRKKY
                     AELTKSTDTITFKGEISAFRQTGRWINHRREVVKNMSSQTWVITDTILDKQKHIMTQF
                     WHPHPDFFSMFEIKAVDAKGEIIQPEITEALYSPTYGIAENCTQIAFHTGGNTITTVI
                     QLLQ"
     gene            complement(10280..10720)
                     /locus_tag="PLM90_03970"
     CDS             complement(10280..10720)
                     /locus_tag="PLM90_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88555.1"
                     /translation="MQPPVIIYRISRFFYDLKIKPISWLFDYSNRLFFKCWIPGSARI
                     GKNFTVGYWGIGVIIHSNTIIGDNCQVNQNTTIGKKITTNGVPKLGNNVYIGANCVIY
                     GAISIGDHAVIGPLSLVNTDIPANTFAAGVPAKPIKENSSSKDT"
     gene            complement(10695..11729)
                     /locus_tag="PLM90_03975"
     CDS             complement(10695..11729)
                     /locus_tag="PLM90_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ88556.1"
                     /translation="MKKALIILPYANPHLGLFIKDISRIYDCDFYIDGPQSKYRNHFE
                     LHDVAIKSETTMKVLGNYFKGKYDVLISHGMFHAFSFFLHIGPRKPLIILSEPLKIPT
                     KAGLLYWLKRSIIRSLKRKHQLNIFILGDLSNSANFQSLAGQEIPCFPYGYFPDWQST
                     TNTKSAHADPVKFVYAGQFIERKNILLLLAGINLYCNKNGHAEFHFAGEGPFKSEIEA
                     NSNITFHGKLDAENVRKLLSASDVMILVSRSEGWGAIVNEAAACGCALMLSEAVEAKY
                     LFLQDGNTGVIVKESPQDIADAITELSHDRNKLQHMKNNVQELYSTIINSHFNKLTKL
                     LNNATSGNHI"
     gene            complement(11726..12805)
                     /locus_tag="PLM90_03980"
     CDS             complement(11726..12805)
                     /locus_tag="PLM90_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88557.1"
                     /translation="MMHTMSYTTSLLLGFTLDMERGVYGLLLLLLYYKYLDRRTIYLN
                     IAVLYISIFTSIYTFDIKAHVQECIGFVLYYNVALIFWKVLGSRQLTPTERLKHFYSF
                     LSIPLLVYISPILLTPNNINLDFYSDPNLEKIGLKSRTVGWASACSIPMVLQWTRKDG
                     PRKFIIYLALAILIVLTIGSGSRSAIIGASLFVLALIYSFNIKQRISYAILLIFFTAF
                     ILQNLDQLSLSRRAELHKMGVSDDTFRQNLVLAFSEHLVEDFPSSLKPEGLGEPNIQH
                     GLNKFFDTKGFGTHNSYISIILGLGFFSIIFLYRMFRGLKKLARLNYLKYFLPFLTIS
                     ITEDCFGPGQLLYFFLIGSMVVLTE"
     gene            complement(12840..13937)
                     /locus_tag="PLM90_03985"
     CDS             complement(12840..13937)
                     /locus_tag="PLM90_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ88558.1"
                     /translation="MTVVIFVTSVYELPNSGPATFAKILSESFHDSEIKFIIVTSDAI
                     NTADDLVVIRRPKSSVALYFQLWRKAKEIAAFYKDDYVIIHYNNSFPYAAFGTFGNRT
                     ILQMNDYYNIRLPYISFLHGSIRRSFAHIFRKLTERLSIHHSDVVLCNSRHTQNEAIG
                     YYKGQHQKFRLLYKSIDDADLGIKTIDEPSGEILYIGNDLKLKGFDILAEALKSTSRV
                     QKLHIAGPAANNPLLNQLLHQVPQHIAIVKHGALTHKELYALSPNLDMLVLPARYEAL
                     GVSVIECLAQGLPVLTSGVGGLMEILEEYPAEFYSASNLKPSILASNIDLILENYKYF
                     YTAVKERIPTIRNKFEKRKMTNYLKTLYLER"
     gene            complement(13990..14808)
                     /locus_tag="PLM90_03990"
     CDS             complement(13990..14808)
                     /locus_tag="PLM90_03990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ88559.1"
                     /translation="MLINAIAFLFQIYVCKYIFRIKIVATLHNIKPHDAKNLHLHFFI
                     YKQFLKCCIKIRVFSNTTIAAASKYYDINTNKFFVIPEGSFVNYYPNNKSKAESRKTL
                     ELPDDAFIFLFLGNIRPYKGIQALLDIYKASEIKDSILIIAGNSPDKAYLEKIQKQTN
                     TSIIIKPGFITTENVQLFYNAANIVVLPFTEVENSGSAILAMGFEKAVIAPKKGVLSE
                     RLSEQPELLYNDGELGLTLKHAAVLSELELRKIGQRNAKHLGKYQWEDFCAFFI"
     gene            complement(15059..15601)
                     /locus_tag="PLM90_03995"
     CDS             complement(15059..15601)
                     /locus_tag="PLM90_03995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ88560.1"
                     /translation="MKTLNLILYSFYLYLINRFITNFPFQAIRRFFFKLLLKKIGTHN
                     SFLMGVQFRNPRNIIIGDNCIINKNVMLDGRNSKLLIGNNVEIAQETNIWTLEHDIHD
                     DHFADKGGEVKIEDYVWIASRVTILPGVTIGKGAVVASGSVVTKDVPSMAIVAGVPAR
                     QIGVRESKLAYNKTHRPWFQ"
     gene            complement(15598..16875)
                     /locus_tag="PLM90_04000"
     CDS             complement(15598..16875)
                     /locus_tag="PLM90_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88561.1"
                     /translation="MAIGVMLTFFDFRNDIRKLISSISKKEKVVVFVRKEHEHLVSSY
                     QSIDFTYRIIDEQQPSIINFLLTRCFLLFRKIPSSRNNYYLMEAFKAEGLPDKKQSRK
                     AKKILKWQKLLPKFFSYDKYLSMLRPSKKTDLGGINSMLVLTEIYDDLLFARIIKSKL
                     PTHVYVYSWDHPCKHVRFSKKVNYLVWNKGISEDLQKLQQIPENQVSVIGSTQLGYIH
                     EYLRQSAQEFPAKQNWIYYACGVGIRSLVHEEVSVIITLAKAMQATIPDHKLLVRPYP
                     NLSNWNLYDALADFDNIIVDSSYRQTDLSVSEKDIFLKFSGIDAANALFHTGTTLGLE
                     ACFLNTPSFIIDIANRKRSDVCLYNFVHQYQNNKYLIEKSPINVVYDEKALHHILQNT
                     ADPIYMHLNKDISSAFEVLSFDDLAANFLNHLS"
     gene            complement(16878..18059)
                     /locus_tag="PLM90_04005"
     CDS             complement(16878..18059)
                     /locus_tag="PLM90_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88562.1"
                     /translation="MGFYGKQLFQLTGNTATICLPVFLLAAANSFFLVIGAVPQQLLQ
                     ANNFFSLRNNWQYICQALNLIALIALWRCGINDVILYSIAIVFISIISLLIDITLISR
                     KMILVGTKLQFKIERKRTSVSQYSFRAFLHAIISFLAVQADKLIIGTVLNVGAVTTYS
                     IITKPYYLLRGMTGTGYAVFQPTLTALQFNSDKSTYLNYSSKIIRTTFIVVMSICCVA
                     GICFEQGLTIWLKTSQYNEMATWGIISLVSVCLTMLYTPFYRTMLYTSSLQTILNFSM
                     LVVPINVVISILLTRAYGFYGVIIGTLIQILLESGFVLFQARKLFNLKYTNIFTKPFN
                     FRFLSILIIFIIGRFCIANITTSGLLISTEITLVLTLILISLNTIRKEGLFSQPINNG
                     N"
     gene            18070..18417
                     /locus_tag="PLM90_04010"
     CDS             18070..18417
                     /locus_tag="PLM90_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88563.1"
                     /translation="MSEMQIAANTDSVIEVLSARLNFIVSLSSRNSTEKRIADLDAPK
                     SNAVNEEESPNARRKRPYVSAPKAFRQIGSVSMPIPSFATYRNRLLDRFLKTLDMPVN
                     NYLLINFVEYTHQ"
     gene            complement(18389..18868)
                     /locus_tag="PLM90_04015"
     CDS             complement(18389..18868)
                     /locus_tag="PLM90_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HMZ88564.1"
                     /translation="MKNKIKVFLTDVDGVLTDAGMYYTASGDEFKKFNTHDGMGMVML
                     QKAGIKTGMLTTEDTPIVKRRADKLKVDYLYQGVGFGGKLAAAQEICTKEGITLNEVA
                     YIGDDINCIELLQAVGLAACPANATPKVKAIAGIIHLQKSGGEGAVREFIDGYILQS"
     gene            complement(18880..19704)
                     /locus_tag="PLM90_04020"
     CDS             complement(18880..19704)
                     /locus_tag="PLM90_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ88565.1"
                     /translation="MKQTRIPLDNKVILLTGAFGLIGKTLSKAILESGAKLVLCDHNA
                     SKIEDMREHLLPFREGADYLICITDITSSSACAKCVDDAVGAFDHIDVLINNAAIDAK
                     FDAQSNDVLNATRFENFPIEQLDRSYEVNIKGLILITQTVVRQMLKQQNGNIINVAST
                     YSLVAPNQSLYDFGSEEIHYKPVDYVATKSMIPNLTRYLATFYAKDNIRCNAIAPHGI
                     FNHHSESFVVNFEKLSPMGRMAGRDEMTGPFLFLASDASSYMTGSVLTVDGGWTAW"
     gene            complement(19701..20663)
                     /locus_tag="PLM90_04025"
     CDS             complement(19701..20663)
                     /locus_tag="PLM90_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ88566.1"
                     /translation="MDNRTPILVIGAGSIGERHIGNLLSLGYENITVYRSRNLPMRTI
                     DTSRIKIITSLADIKTIPFEAAIICTPTSLHAEQTLFCLEQGMHVLVEKPLSHKTHQL
                     SEIAETCKRTGKLMQVAYMMAFHPHLQKMQKAIEEESFGKLLFIDTYWGSYLPDWHPW
                     EDYKQGYAARKDLGGGAALTLSHDLDMANRLANAPVAQFSKQYQYPKHLDINVEGIAD
                     FQIRYMNNVVAHVHMNYVDRVPRRTYRCVFEDGCMEMDFFAKTLTTVTATSTNKETLE
                     DFDRNDLFIAELNHFFDRIQSNTDHSAYSFDQILHSATIIEMCL"
     gene            complement(20656..21381)
                     /locus_tag="PLM90_04030"
     CDS             complement(20656..21381)
                     /locus_tag="PLM90_04030"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HMZ88567.1"
                     /translation="MEVLAIIPARGGSKGLPGKNIRPLNGHPLLAYSILAAQQSELIT
                     RIIVNTDDLDIAAIAQQYGAEIPFMRPANLALDNTTDFEVFSHQLKWHLENEQYRPDI
                     IVQLRPTSPIRMPGWIDDGIRAVVERNADSLRIITEAPNTPYKMWTLGADKHAAMEPL
                     LSINNIDEPFNMPRQQLPKVYWQVGTLDVIATSTIEAGGMSGKKILPMIIDASLSVDI
                     DDLASFEKAEKIIAAQPCIKFHG"
     gene            complement(21388..22272)
                     /locus_tag="PLM90_04035"
     CDS             complement(21388..22272)
                     /locus_tag="PLM90_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HMZ88568.1"
                     /translation="MFQFDYRMPKVIAEIGCNHMGDFDIAKELIRLAKECGADYAKFQ
                     KRNPKELLDNNQYNAPHPEPWQSYGDTYGAHREFLEFTVEQHAHLKSYAESIGIGYSA
                     SVWDITSASELIGLNPDFIKIPSASNTNFALLEILRDTYNGDIHVSVGMTTSDETEKM
                     VSFFEQTNSAKNRLVLYSCTSGYPIQFSEICLLEINKLYQQFGERVKEIGFSGHHLGI
                     AADIAAYTLGARWIERHFTKDRTWKGTDHAASLEVPGLQKLVRDLHHMQEALQYKYED
                     ILPVEQAQRNKLKFKSGN"
     gene            complement(22338..22790)
                     /locus_tag="PLM90_04040"
     CDS             complement(22338..22790)
                     /locus_tag="PLM90_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88569.1"
                     /translation="MAKEMHESTNAVRLELNKLSKAGFLVSYGEGNTIQYKANDKHPL
                     FPELNSLVRKYLGIDKIVENIVKQIGEIHYALITGDYAEGKDTGNIDLMIVGEVDPER
                     LQYWVLKTETLIKRKVLTTVVNEQTFASYKENGILNSSIVLWSNGVAE"
     gene            complement(23004..24059)
                     /locus_tag="PLM90_04045"
     CDS             complement(23004..24059)
                     /locus_tag="PLM90_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HMZ88570.1"
                     /translation="MDEKKFFQGRGSQINPFNPYHDINYAQEHIEGLDESWPAPGETQ
                     FFTEYPKRIVNFVDSPDSPMGISVNPYQGCEHGCVYCYARNTHQYWGFSAGLDFETRI
                     ITKPDAPKLLEKAFNDKNWVPAPVSFSGNTDCYQPVERKLRLTRGLLEVCQRYRNPAG
                     IITKNRLILRDLDILEELAQHDLIKVYISVTTLDESLRRAMEPRTSTAANRLDILKKL
                     SEKHIPVGIMIGPVIPGLNDHEIPALLAAAAEHGAFDAGFNMVRLNGAVGQIFSDWIH
                     KAFPDKAERVLNNIRAVHQGQLNDSRWKLRMRGDGHLAESIHKLFRLSRDKYMPERKM
                     FTYNTSLFRRPGQMRLF"
     gene            24137..24925
                     /locus_tag="PLM90_04050"
     CDS             24137..24925
                     /locus_tag="PLM90_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HMZ88571.1"
                     /translation="MSNIASNLRHLRGERKLSQQELADMIGIGRTTIANIESNLANPG
                     HKTLLEIAKVFNVTIDELLHADLTKRILIQEKPAQHVAFRPVVVTVDELGQENIVLVD
                     TKAAAGYPTRYLEPEYYRELPAFKLPGSDYRNGTFRCFQVDGDSMQDTIQHGDFVVGR
                     FCDNHFKDVRDGYIHVVVTNDNVLVKRIISRAESEHKLYLISDNDAYPEIVVDIDDVR
                     EIWFVKSKISSYMPVKKNDLDKVIGDLTMQVHLLKSRIDQLEKN"
     gene            25170..25547
                     /locus_tag="PLM90_04055"
     CDS             25170..25547
                     /locus_tag="PLM90_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HMZ88572.1"
                     /translation="MIAPSNIITNRQRIAIGEELIWQQLKEKRVFGLTFARQYPFYLQ
                     QPDGERLVYSADFYCHDAGVAVRIVNCDAITAEYNEQMAWCKQQLQMRGILVIELPCD
                     AIADLKQVIAVIRRELKYKTIHA"
     gene            25636..26463
                     /gene="kdsA"
                     /locus_tag="PLM90_04060"
     CDS             25636..26463
                     /gene="kdsA"
                     /locus_tag="PLM90_04060"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HMZ88573.1"
                     /translation="MLPVFTNPVQITPEILVGNGKPLLIAGPCVIESKALCLQVAETM
                     KEVAARTGFQYVFKASFDKANRTAGGSFRGDGMEAGLEILQHIKETVGVPVLTDIHLP
                     DQAAIVAQVVDVLQIPAFLCRQTDLLIAAGATGKAVNVKKGQFMSPEDMAYAVEKVAS
                     TGNANALLTERGASFGYHNLVVDMRSLPIMRQYAPVVFDVTHSVQMPGGAGGKSGGKR
                     EFAPYLAKAAAATGVDGFFIETHPNPDQALSDGPNMIPLHEMENLLRTLKAIIDIVG"
BASE COUNT         7713 a         5213 c         5216 g         8396 t
ORIGIN      
        1 tactgttttg gcactttccg cttttcgatg taaactcatt tttaaaatct catcggcgat
       61 gccattgact acatcctggg ttttttgcct gtttttgtcg cggtatttaa tgcttatttc
      121 gccgccatat aaattttcaa catcaataga aacgtcttct tggatacgct cagactcttt
      181 taaaaggtcg ttgatagtga aaaagtattc gaacttgaat tttgatttgg caaatgtatt
      241 gagggttgta ttgatattaa atgcaggggt gctatatgct ttattaaacg agaatttttt
      301 agaaaacttt tcatttttat aagtataact caatttgtat tctgaggtat ctagtatttg
      361 aacatcaatt ttatcgccta ttattgctga atctaaaatg gcaatattta ccgtaaatgg
      421 cgattcgtta tagatttcgg cagctttaac tctttcacga atataatatg acacaccaat
      481 aggtaaatct gaaagtgctg taattttata acccttagaa ctaagaacag tcatttcctc
      541 gttcatgcgg tcaatattgg aattggctac ccttatgcct aagtcgatag tattgtcttt
      601 ggatttatcc ttaaactgaa tatcggcatg agactcataa ataggtgtgg agtagcgatt
      661 gtataagaat gcagcacaaa cgccaattgc aaaaacaagc actatccacc agcgattaat
      721 ccaactgatg gtaaggaata aacgccagtt aaatgactga tcattttgct gttcaacgct
      781 tttatggaat tctttattcg gtgaattcat tccttattga ttgacatttg cgaaaaatgc
      841 gtaaataaca gcaatgctgg aaatggtgct taaaataggc ccaatttcct gaaatacggt
      901 actattgata tttggttcga tatagataat atcattcggt tgaatgtaca tattgcctaa
      961 agcaatatca tcaatgctgc ttaaatctat ttcttgtaat tgcgcagcac cttgaatatt
     1021 gcgaattact tttattttcg atgatcttcc attttcagga ataccaccgg ccatacctat
     1081 tgcttccaat aatgtaaggt ctgagcgatt taatgctaat tgtttagcat cgctgcctcc
     1141 gcgataaacg gtaatgtact tacctgtaat aaccacattg acaaaagcat cagtatatat
     1201 tttaccatac tcagtggtca gcattttttc tgctcccgat tcggttaaac cggagatttt
     1261 catccacccg aggcgaggta agttcgccat tccgctgcca tcaatctggt agcttagtga
     1321 ttgcagcatg gtttgcggtt gataataatt catttgattg ctaatctgcg attcgatcag
     1381 attgtatccg ccattaggat atacaaatac ctgaatttga tcacccggtt cgataacaaa
     1441 ctcgcgataa atggtatcta aaaacgacga ttcaacaaaa tccttcccgg tatccaacat
     1501 gcgattgggg tacagcattc cacatgatga aaatgtagcc agcagcatac ctgcaagtaa
     1561 taaatggata atcggccttt ttgtcggctg tttagagaaa ctggtcatac tgtatgccct
     1621 gaggcgaact tagcctacaa atatagcata ttcggcgtat tagtttaaag ggcgctgatt
     1681 gccctgaaac cttatttata agcggtatcc tgcttgtcta agcgttcgaa ttccgaattg
     1741 ttactcttgt attcgggtat cagcagtttt aggagcctga ctgactgctg gatatcagaa
     1801 tttacaattg catcttgcag catttgtaag taatgttgga cttgctcggc agggtagggg
     1861 gctgtgagtg cgatgcgtat tttttgatga taggtaggta aggtgttctc gtttgaatgc
     1921 aacaattctt cgtgtaattt ttccccctgt cgcaagccgg taaaagtaaa ttggatgtcc
     1981 tttccggggg tatatccgct cagtagcacc attttggtag ccagatcggt gattttgacc
     2041 ggatcgccca tatcgaacat gtatatctct gatgattttc ccatcactgc ggcttcaagt
     2101 actaattgac aggcttcagg aatggtcatg aaatatctgg ttacatccgg atgcgtaatg
     2161 gtaagtgggc caccgttttc aatttgtttg ttgaaataat tgacaacaga accattcgat
     2221 ccgagcacat tgccaaatcg cgtagtaata aaagtggttt tgcgcgaatg ttgtgatcgc
     2281 tcttgtacat atatctcagc agctcgttta gttgcaccca ttacgcttgt tggattaact
     2341 gccttgtcgg tagagatcat gataaattgc tctacttcgt attgaatagc aaggtcggtt
     2401 aaatacatgg agccgatcac attaacgtga gtggcctcaa tagcatttaa ttccatcaga
     2461 ggtacatgtt tatatgctgc tgcatgaaaa acaaatgcag ggtgatatgt cgcgaaaata
     2521 tcttccagtc tttttcggtt ggtgatgtcg gcaataatat tttctgtaac tatctttttt
     2581 tcatttaaac gaaattgcaa ctccatatat aaatcataga gatcgctttc ggcttggtcg
     2641 acaagaatga gttgcttggg ttgaaatggt atcaactgcc gcactaattc acttcctata
     2701 gatccggcag cacctgttat taatattgtc tttccaccga tagcagaaag taatttatcg
     2761 ttttcgaggt gtatttgttc tctccccaat aaatcttcaa tattgaattt cctgagttga
     2821 cgggtgctta atttattatt gatccaatcg tcgaccggcg gcactgattt aataaatacc
     2881 tgatgttgca ggcaagcgtc aactacagcc atttttttat caggtattgg atgggcgata
     2941 ctaataatca gctcttttac aacaaatcgg cgcaataagt cgggtaattt ctcggagtta
     3001 taaatcctaa cgccttcaat cgttttttga aatttcgaag ggttgtcgtc gagaaatgct
     3061 gttatttgat agttgtgatt ggtatctctt ctcagtgtgt gcattacgct caagcctcgt
     3121 cttccggcgc cataaataat tacaggaatg gcagcagcgt tattgcgggt ggcttgataa
     3181 aaaagcactt ttacaacata gcgtgaaaat aataaaaagg cgatattgat agacgtatgg
     3241 ataatggcaa tagatgcggg caacgtaaga aacggttttc ccgtttctct gccgatgatg
     3301 gatagcgcca aacaaacaat agtagccaac atgcctgaca tcactattct tctggcttca
     3361 gcttcgcctg aatgtcggat aatgccatcg taggtcttaa atgccagata acttgcagta
     3421 aatactaagg tagtaagaaa ggtgttatca acgaaagtag aaaatgaaat aatcgctatg
     3481 ttgaaattat atcgaagaat atatgcaaca ccatatgtaa atatggtaat tactatatcg
     3541 aagcaaaata ccaaccactt ggacaatatt cttgttccaa aattttgatt gatataatta
     3601 ctccatctgc gctccatttg cctgatagat tttattccaa ttagcggaaa ttaagataat
     3661 gcttacgcac acctctgtca caaatctacg atattttaac acttttatgc gtctatttca
     3721 cagcagagcg aatgagatca atgatctcct gctgttgggc tgcagtaagc gaagagcctg
     3781 aaggcaggca aagcccatat tgaaagatgt tttcggcgac accttgattg taggtagttg
     3841 catcattaaa aaccggttgc aggtgcatgg gtttccatat cggtcgcgat tctattcctg
     3901 atgctccaag tagcaatctg atttgctccc tatcagtccc tgtttgatct gggttaatca
     3961 aaattgtggt aagccatctg gaagacttca tttgttccat ttcagtagaa aatgaaatgc
     4021 cttcaatatc cttcagatgg ttatagtaat aatgaaatat ttcgcgacgg cgttgaacac
     4081 gttctgatag cacagtcatt tgtcctctgc cgatggctgc gcaaatatta cttaatcggt
     4141 aattatagcc tatcactgaa tgctggtaat gaggagcgtt gtctctcgct tgtgtggcaa
     4201 gaaatcgcgc atagttggct tgctcttttg ttttgcataa caaagcacct cctcctgaag
     4261 tggtgattac tttattacca ttaaatgaga gaatgccaaa ttcgccaaat gttccggtgt
     4321 gttgattgcg atatgttgct cctagtgatt ctgcagcgtc ttcaatcaat ggaatatcat
     4381 attgatgtga gatgcggatg aatgcatcca tgtcggaagg catgccatac aaatgaacta
     4441 aaattatagc ttttggtttt ttccctttgg cgattaattc tttgatggca gactcgcata
     4501 aatttggatc catgttccag gaagtcgcat cacaatcaac aaaaacgggc gttgcaccct
     4561 ggtaaataat tggatttgca gaggctgaga aagtgagtgt ggggcaaata acgatatcac
     4621 cttttccaac actaagcatg attaatgcaa gatgcaaggc tgcagttccg gaacttaatg
     4681 cagcgcaatc attgctgcct gtaaacgcag ataattcttg ttcaaaacca tcaacatgcg
     4741 cgcctagtgg agcaatccaa ttgctgtcaa atgcgtcgtt aacaaacctt tgttcctcac
     4801 ccgacatgtg cggtggggat agatagattc tgattttatt gacagcgctc attcgttaca
     4861 gtcggtacaa aggaaaaaaa tcttttgcac tgttttgcat attggaaatt ttatcagcag
     4921 aaagagattg atattttatt tcatcgcaaa taattctaag ggtgctgaaa gttgctcttt
     4981 cattggagaa gccggcttta aacctaaata aactatcttc agcggatcct acgccaccgc
     5041 ctaaatgtat ggctttccgg ttgtctagtt taaaatagtc tatcgcgtgg tcaaatatca
     5101 attttgaacc tgcaagatgt cttgtagatt gttttgttcc tcccaaatga tattgaaccg
     5161 tattgccatg tgcaaaaaat aatgcccctg aaataaatgt gccgtcaggc gtatgcgatg
     5221 aaattaatat actattgaat tcaggttgtc taagtgactc aaagtatgat cttgaaaaat
     5281 aataaaaatt tgatgcctga tgaagtgcca ttgtttcttt gtaagcctca atgaactcat
     5341 cgtaatattc ccatgcatta aattttgtct cgatcttcaa ctgcattaat ttgcgcaaat
     5401 cacgctttag tccggtgcta tatgatacct ttatatactc aaccggattt tctaaattga
     5461 tatagacagt gttgccatgt attacctgat ttaagcttgt agttggcgga ataatcaact
     5521 tttggtgaat gggatgcatt cgaataaaaa tgctgactat atttttttta gaggcttcta
     5581 atctgcaggc atttaatgcc tcggtaagct gaccgatatc agaaatatta gttagaattc
     5641 ccgcataacc gtaagggctg atggcatcaa aaagtttttc acccttggcg attgcatccg
     5701 gaatttgtct taataataag ggcaatgcca aagcaccatg agcgcctgtg gcagtgatta
     5761 atattggctg agcatgaatg cttaatgcat ccatctcaca ataggatggg atttggtaaa
     5821 catcatcagg ttgcaagcct aatgaggtga tctgactgat atgattatat ggagatatcg
     5881 ttatgtgcat ggttgaacat tttcatacag atcacatcct gaggttctcc atttttaatg
     5941 gtttgatcaa ctaatacctc ctcatttgta aagcccattg agcgatataa cgaaattgcc
     6001 cgttcattaa tttgcaatac atggagcttt atagtttgaa ttccgggcgt attaaaggcg
     6061 atttgaatca tagatgaagt ggctgcgcgg ccaattcctt tgccccagta ttccggtaac
     6121 ccgatcagga ttccgaatat acaggtgcca tcttcgggat ttatgtcata cagacctgca
     6181 tgtccaatca ccttgcgatt gattgtatct tcaataatca gtatgagatt atttggattt
     6241 gattgatggt gtcgaatcca attaataaga ccttcctttg tataaaccgg agggtctttt
     6301 tcaagccata ccgaagcttc tttattgttc tttacctcaa gtaatgagcc tgcatctgaa
     6361 atatatagtg gtcttaaaat aacaccttct gaaatgtgaa tttgatttcc tttgcgttct
     6421 atgtcaaaat cagcgactac agttcgtgga tctatatgat atttacttcg cttgagtaca
     6481 acgataaatg tttgaataaa aatttcaagg tccattttga aattgtatga tgcagcatat
     6541 acaacatcga aatttaatcg cgcttcccag tcgagcgtat ttctgccatt tatttgtgcc
     6601 aatccggtaa gtccgggctt aacatcgtga cgatgccatt ctgccgaggt gtaatatggt
     6661 atgtatctca ctaataatgg tcgcggacca atcaaactca tatctcctat caagatgtgg
     6721 aatagttggg gtaattcatc caggctcgac tttcgaagaa aatttcccca ggcggtcaac
     6781 ctttctgtgt ctgcaagatc tttaccggca ggctgcggac gcatggtgat gaatttatac
     6841 aatctgaaaa tggcaccgtt tttacctacc cgatcttgta taaagaatat ttgtcctttg
     6901 aatgtgatag cacaaacaac tgctatgatg accatgaaag gtaaagtgat ggcaatcgcc
     6961 gtgagcgaaa aaactatatc aaggaatctt ttaataaaat gtttatacac gattgctatt
     7021 tagaacagcg ccctgtaagt tcacgaatgt attgttgggc cagtgttttt ctatcgaaat
     7081 gttgtttaac atatgcatag ccattttcgc catgactttt aattagttca ggatgttgca
     7141 gatacgttga aatggcttta atcatttcat cagtattttc aggttctaca tagataccgg
     7201 catctgcatc ttcaattaat ttgcgggata cgccatcaat cgccatgatt accggcttct
     7261 tacatgccat gtaatcaaag gttttattgc tataaactgt tttaaaagta tctgctttct
     7321 tcaatacaga caaaccgata tcacaagcca gtatatattt taaagcttcc gactttgtga
     7381 tcaacccgat aaaggtcaca ttcttgaggt tcctagcagt aaccatttca atgagtacct
     7441 gtttttgcat gccatcgccg ataaaaacga acattaccgg gaggtctctc aatttttctg
     7501 cggctattat caattgctcc aaatgatttg caacgccatg tgcacctaca tagcaaagcg
     7561 ataatttgcc attgagttgc agtgtttcgc gaagctcagc ggtatcaaag tttaaaagca
     7621 gtacatcgct catgtcaaaa tccgcggcat tcgggatata aatgacttta tttgcgggaa
     7681 cttttttgtt gttgataagg acttcctgca ttgccggagt tacaacattt gttacaagtg
     7741 cttttctata taataatgcc tccaggcgat atgaccattt gatcatggtg ctgttcgtga
     7801 gcactccggt gtcaatggca gattcgggcc ataaatctcg tatctccaat gcaaatggtc
     7861 tgcgtttgaa gaaggcaagt gtcaaagcaa taatacccac cgaaagtggc ggcgaactca
     7921 ctaaaatgag atcatattta tctcttgctt tgaatagacc tccataaatt ccggaaaaaa
     7981 caaatgaaaa ataggcattt aatctgccac gaaaactttt attgtatgat tttgatacat
     8041 gtgttctgat gacgcgaata ttagtatcaa attgttcgga ataggtgtat ttgttagtat
     8101 acctcggatg tttaatgcca ctcgcataat tagtcatacc ggcaatcact gtaatctgat
     8161 gaccttcagc cgcccataac ttagtcattg cattccaacg catgccaccc gggtcatctt
     8221 tttcctgata atattggtga atgagtaaaa tatgcattgg gttgatcggg ctacagcctt
     8281 tgtggtggct gggggataaa ggtaatcacc aattaatata tttcaaaaat gtgaaattga
     8341 tatcggcgac aataatttga acggatgttg ctttaattcc ttattgctca cttatactat
     8401 tgcaatagct gaattactgt agtaattgtg tttccacctg tatgaaaggc aatttgagtg
     8461 caattttccg caatcccgta tgtgggtgaa tacaatgctt cggtaatttc aggttgaatt
     8521 atttcccctt ttgcatctac ggctttaatt tcaaacatgc tgaaaaaatc cggatgtgga
     8581 tgccaaaatt gtgtcatgat atgtttttgt ttatcgagga tggtatcggt aatgacccat
     8641 gtttggctgc tcatgttttt taccacttct cttctgtgat taatccatct tccggtttgc
     8701 cgaaaggcgg aaatctctcc tttgaaagtg atggtatctg ttgatttggt aagttcggca
     8761 tacttttttc ttgtccaaaa atgccagaca aaacgaggac ccttcagcat ttggtctaat
     8821 ccgtcaatgg aaacagtatt gtgtcctttt gttcccataa aaaacgcagt gacttcgggt
     8881 gtgctattat attgataagt tccggcatca cgcatgatat tcattccatt gtaaattatg
     8941 tcaatgtgta aattgtcggc ttgcgatggc ctgaatgggt ggtctccaca tcgaatgaag
     9001 gtcttagaat actgttcatt aaaaatgtaa taaccacctt cgttaaaatg gtataaatct
     9061 tgtttcggta tttctgtttt gctaccggat tcaatgtcga atccaaacca ttgtgcatct
     9121 tcaataagtt gaggataagg gttatttgca cccaaggcaa tgctgaatgt tgctatgcat
     9181 gtacgataat cgttgtaagg cgtatcggta aaaggaaata aagatgttcc atcattgttt
     9241 ccataatttg caacagtccc tgatttttca tcaataaagg cattaacaaa ttccagcgtc
     9301 gtcaatcctt tctggattgt ttcttgttga aaatggtgac cgttaacttt tcctacgcgc
     9361 aacatcagtg aaaataaccg tatcaccatg cgatggtaat tcattgaata ttgaagatag
     9421 ctgccatcgc cggcaatttg gtaattaatt tctttttcaa agagactcca gccaactttt
     9481 cggaattttt ctgaatcagg gaattgtggg aaaaaagtgc ctatgatgta taaacctgca
     9541 gcttctgaaa gtacatggtt gtttcggacg aggtttttgg aaaaatcgag gtatttgaag
     9601 atatgtatag cctgcgtatg aatagacccg gtgatctttt ggtagacttc tgttgttaga
     9661 ctgctgctat gtttatatat atctaaaaat attgtccaat tgataatacg tatcgctatc
     9721 tcctggcttg acatgtaatg tggtccggcg ttcatttccg cgtgatcgat aaacgagcag
     9781 atttcctgcc atatacatgc actgttatca gtgccaaagc attgatcata ttgtaatact
     9841 gcatacagat aattaaatct tgccggctcc cagatgtatt taatatcttc ctctgaggca
     9901 aaatcattca aattggccca atgtatcttg ttgtcgtata ccttgcctgt ttttgcattg
     9961 gttgtccatc ggttttctcc ggtaaatttc aaaacatcac cgtcaaaaaa ttgaatatgc
    10021 ccattgttga catcagtaaa tcgttgagtt actgtttctc tattattttc aatctttgta
    10081 tggtccaaat gtatattgcc aaaagttccc caaggtgcga ttgtgaaatc cttggatatg
    10141 tcggatggtt caaaagcttg gatagggaat tgatattgca gtataccggt tcgtttcttt
    10201 aggccatgat agaatctgta agcccagtat ttgaagccaa cgcctttgaa taaatcaacg
    10261 tatagttcaa tgaagcgaat catgtatcct ttgacgatga attttcttta atgggttttg
    10321 caggaacacc tgctgcaaat gtgttcgccg gaatgtctgt atttaccaga ctaagtggtc
    10381 caataactgc atgatcacca attgaaattg cgccatagat cacacaatta gcaccgatgt
    10441 atacattgtt tcctaatttc ggcacaccat ttgtggttat tttcttgccg atggttgtat
    10501 tttgatttac ctggcaattg tcgccaatga tggtattgct gtggatgatt acccctattc
    10561 cccaatagcc aaccgtaaaa tttttaccta tcctagccga acccggaatc cagcatttaa
    10621 aaaataatcg attgctgtaa tcaaacaacc atgatatcgg ttttattttt agatcgtaga
    10681 aaaaccttga aattctatat atgattaccg gaggttgcat tatttagtag tttggtgagc
    10741 ttgttgaaat gggaatttat tattgttgaa tacaattctt gaacattatt tttcatatgt
    10801 tgaagcttgt ttctatcatg tgaaagctca gtaatggcat ctgcaatatc ctgaggcgat
    10861 tcttttacta ttacaccggt attaccatcc tgtaagaaaa gatatttagc ctcaacagct
    10921 tcggaaagca ttagcgcgca accgcaggca gcggcttcat taacaatggc tccccatcct
    10981 tccgaccggc tcactaagat catcacgtct gatgcactaa gtaatttacg cacgttttcg
    11041 gcgtcaagtt taccgtggaa agtaatattt gaatttgcct caatttctga cttaaaagga
    11101 ccctctccgg caaaatgaaa ttccgcatgg ccatttttgt tgcaatataa attaatccct
    11161 gcaaggagaa gtaatatgtt ttttcgttca ataaattgtc cggcatatac aaattttacc
    11221 ggatcagcat gagcagattt tgtatttgtt gttgattgcc aatccggaaa atagccatag
    11281 ggaaaacaag gaatttcctg ccctgctaaa gattggaaat ttgccgaatt tgatagatcc
    11341 cccagaataa aaatgtttaa ttgatgtttc ctttttagtg atctaatgat gcttcgcttg
    11401 agccaatata acagaccggc cttagttggt attttcaatg gctcagataa gataatcagc
    11461 ggttttcgag gtccaatgtg aagaaagaag ctgaatgcat gaaacatgcc gtgagaaatc
    11521 aatacatcgt atttgccttt gaaataattt ccgaggacct tcattgtggt ttcgcttttt
    11581 attgcgacat cgtggagctc aaaatgattg cggtatttag attggggtcc atcgatgtaa
    11641 aaatcgcaat cgtaaatgcg gctgatatcc ttaataaata agcccaaatg tggattggcg
    11701 tagggcaata taataagggc ttttttcatt cagttagcac aaccatgctt cctatcagaa
    11761 agaaatataa caattgcccc ggcccaaagc aatcttcagt tattgaaata gtgaggaaag
    11821 gaaggaaata tttaagataa tttaatcgtg cgagtttttt gaggcctcta aacattctat
    11881 aaaggaagat tatggaaaag aatcccagac caagtatgat ggagatgtat gaattatgtg
    11941 ttccaaatcc ttttgtgtcg aaaaatttat ttaatccgtg ctgaatattt ggttcgccaa
    12001 gtccttccgg cttaagtgat gaagggaagt cttctacaag gtgttcggaa aacgctaata
    12061 caaggttctg cctgaaggtg tcgtctgata cccccatttt atgcagctct gcgcgccgtg
    12121 ataagcttaa ttggtcgaga ttttgaagaa taaatgcagt aaaaaaaatc aataatatgg
    12181 cgtatgaaat tcgttgctta atgttaaaac tatagatcag ggctaataca aacaatgagg
    12241 cgccgataat agcagatctg gagccggagc cgatggtaag cactattaaa atggccaatg
    12301 ccaaataaat aataaatttc cttgggccat cttttcttgt ccattgtaaa accatgggaa
    12361 ttgaacatgc ggatgcccaa ccaactgtgc gcgattttaa accaattttc tcaaggttag
    12421 gatctgaata gaaatcaaga ttaatattat taggggtcaa caatatcgga cttatataaa
    12481 ccagtaatgg tatagacaga aagctgtaaa agtgttttag cctttctgtt ggtgtcaatt
    12541 gtcgactacc caacactttc caaaaaatca gtgcaacatt gtaatatagt acaaagccta
    12601 tacattcttg cacgtgagct ttaatatcaa aggtgtatat tgatgtaaat atagagatat
    12661 atagaacggc aatattcaga tagattgttc tacgatctag atatttataa tataacagca
    12721 ataacaagag accgtaaact cctctttcca tatcaagtgt gaacccaaga agtagtgagg
    12781 ttgtgtatga catggtatgc atcatgccaa acaggtaaaa cattatatat gttgttacat
    12841 tacctttcaa gatataatgt ttttaagtaa ttagtcattt ttcttttctc aaatttattc
    12901 cgaatggtag gaattcgctc ttttactgcg gtgtaaaagt atttatagtt ttctaatata
    12961 aggtctatgt ttgaagccaa tattgatggc tttaggtttg atgcagaata gaattcagca
    13021 gggtattctt ccagtatttc cattaagccg cccacccctg aagttaatac ggggagtcct
    13081 tgtgccaaac attcaataac agaaacaccg agtgcttcat atcgggcagg cagcaccagc
    13141 atatccagat ttggacttaa tgcgtataat tctttatgcg ttaatgcgcc atgttttact
    13201 attgcaatgt gttgaggcac ttgatgtaat aattggttta ataaaggatt gttagcggcg
    13261 ggacctgcaa tatgcaattt ttgcaccctg gatgttgatt ttaatgcttc tgccaatatg
    13321 tcaaatccct ttaacttaag atcgttgcca atatataaaa tttcacctga tggttcatca
    13381 attgttttaa tacctaagtc ggcatcatct atgcttttat aaagcagtcg aaatttctga
    13441 tgttgacctt tgtaatagcc aatggcttcg ttttgagtgt gtcgactatt acacaatact
    13501 acatcagagt gatgaattga caatcgttca gttagtttac gaaagatgtg tgcaaatgac
    13561 ctcctaatgc taccatgcaa aaatgaaata tatggcaagc gaatgttgta ataatcgttc
    13621 atttgcagaa ttgttctgtt cccgaaggtg ccaaatgccg cgtaaggaaa tgaattatta
    13681 taatggataa ttacatagtc atctttataa aaagctgcaa tttcttttgc tttccgccat
    13741 aattgaaaat atagtgcaac tgatgatttt gggcgtcgaa ttacaaccaa gtcatcagca
    13801 gtatttattg catcagaggt gacaataata aacttgattt cagagtcgtg aaaagattcg
    13861 cttagtatct tcgcaaaagt ggcagggccg gaattgggca attcatatac actagtaaca
    13921 aatataacca ctgtcattcg ctcttccact atattgatta ttaattcact tttatagaat
    13981 tatgtcctgt tatataaaga atgcgcaaaa gtcctcccat tggtatttgc ctagatgttt
    14041 tgcgttcctc tggccaatct tccttaactc caactcggaa aggactgcag catgctttag
    14101 tgttagacct aattcgccat cattatatag taattcaggc tgctcgctaa gtcgttctga
    14161 taatacacct ttcttgggcg caattacagc cttttcaaaa cccattgcta atatggcaga
    14221 ccctgaattt tccacctcag taaacggtaa aacaacaatg ttggctgcat tataaaataa
    14281 ttgcacattc tctgttgtta taaagccggg cttaatgatg atactggtat ttgtctgttt
    14341 ttgaatcttt tccagatagg ctttatcagg gctgttgccg gcaatgatta aaatactgtc
    14401 ctttatttca gatgctttat aaatatctag taatgcttgt attcctttgt atggtcttat
    14461 atttcccaaa aatagaaaaa taaatgcatc atccggtaat tctaatgttt tcctggattc
    14521 agctttggac ttattatttg gataatagtt tacaaagctg ccttcaggaa taacaaaaaa
    14581 tttatttgta tttatatcgt aatatttaga tgctgcagcg attgtggtat tgctgaagac
    14641 ccttattttt atgcagcact tcaagaattg tttatatata aaaaaatgca aatgtaaatt
    14701 tttggcatca tgtggcttta tgttatgtaa tgtggcaaca atctttattc taaatatgta
    14761 tttacagaca taaatttgaa aaagaaacgc aattgcattt atcagcatca atactttatt
    14821 cctgcgtgca tataagctgt ctatccagtc gaaatgtata taatcgggaa atgagctgat
    14881 tgcagttaag aagatgccaa ttagtctatt tttgttaccg gaataggcgt gtaatgtttc
    14941 attggtgttt agtccttgga tcatgagata ttgaaatgga tttccaattc cggcaacagg
    15001 taaacaggct attttaacct ttcgagtcat ctgctttttc gtttaactga agatagtttt
    15061 attgaaacca agggcgatgg gttttattat aagcaagttt actttccctc acgccgattt
    15121 gacgggcagg aacaccggca actattgcca ttgaagggac gtcctttgtt actactgatc
    15181 ctgaagctac tactgcgcct tttccaattg taacgcccgg aaggatggta actctgctcg
    15241 caatccagac ataatcttca attttcacct caccgccttt gtcagcaaaa tggtcatcat
    15301 gaatgtcgtg ctcaagtgtc caaatatttg tctcttgtgc aatttcgaca ttgttgccga
    15361 tcaacaattt actgttcctg ccatcgagca tcacgttttt attgattatg caattgtcgc
    15421 cgataataat attcctggga ttcctgaatt gaacccccat cagaaaacta ttgtgagtgc
    15481 caattttctt taataggagc ttgaaaaaga atcgccttat agcctgaaag ggaaaattgg
    15541 taatgaatcg atttattaaa tataaataaa aagaatatag tatgaggttt aaagttttca
    15601 tgataagtgg ttcaggaaat ttgcagctaa atcgtcgaag gagagaacct cgaatgctga
    15661 tgaaatatct ttatttaagt gcatatatat gggatcagcc gtattttgta gaatgtgatg
    15721 aagcgccttt tcgtcgtaaa caacatttat aggagatttt tcaatcagat atttattgtt
    15781 ttgatattga tggacaaagt tgtataaaca tacatcagac ctttttcgat ttgcaatatc
    15841 tataatgaag cttggcgtgt tcaaaaagca ggcctccagt cccaatgtgg ttccagtgtg
    15901 aaataatgca ttcgctgcat caatgcctga aaatttaagg aaaatatcct tttctgaaac
    15961 ggacaaatcg gtttgtctgt aactactatc tactatgata ttatcgaagt cagctaaagc
    16021 atcatacaaa ttccagttac ttaaatttgg atacggcctt accaatagtt tgtgatccgg
    16081 gattgttgcc tgcatggctt ttgcgagagt aatgattacc gatacctcct catggaccaa
    16141 tgagcgaatt ccaacaccac aagcataata tatccaattt tgctttgcag gaaattcttg
    16201 tgccgactgc ctcaaatatt catgtatata tccaagttgt gtagaaccta ttacagatac
    16261 ttggttttcc ggtatttgtt gcaatttttg caaatcctct gaaattccct tattccaaac
    16321 taaatagttt acctttttac tgaatcttac atgtttacaa gggtgatccc atgaatacac
    16381 atagacatgt gtaggtaatt tacttttaat gattctcgcg aatagcagat cgtcgtatat
    16441 ttctgttaaa accagcatgg aattgattcc gcccaaatct gtttttttcg acggcctgag
    16501 catgcttagg tatttatcat aggaaaaaaa cttgggaagc agtttttgcc atttcaaaat
    16561 ctttttagcc tttctgcttt gttttttatc cggcaacccc tcagccttga aagcttccat
    16621 aaggtaataa ttgttcctgc tggacggaat ttttctgaat aataaaaaac aacgcgtaag
    16681 caggaaattg ataatggatg gttgttgctc gtcaatgatg cgataggtga agtctattga
    16741 ttgataactg gaaactaaat gctcatgctc cttgcggaca aagactacaa ccttttcttt
    16801 tttggaaatt gatgaaatga gtttgcgtat atcatttctg aaatcaaaaa acgttagcat
    16861 gacaccgatt gccatatcta gttgccgtta ttaatgggtt ggctaaaaag accctctttt
    16921 cttatagtat tgagggagat tagtatgagg gtcaaaacaa gggttatctc agttgaaatg
    16981 agtaagccgg atgttgtaat gttagcaatg caaaaacgac ctatgataaa aattataagt
    17041 attgacaaaa agcgaaagtt gaagggcttg gtgaaaatgt tggtatactt taaattaaat
    17101 agtttccgtg cctgaaatag cacaaatccg ctttctaata aaatttgaat caacgtgcca
    17161 ataatcacac cgtagaaacc ataggcgcgt gtgagcaaaa tactgattac aacatttatt
    17221 ggcacaacta acatggaaaa atttagaatc gtttggagag atgaggtata caacattgtt
    17281 cgataaaacg gcgtatacaa cattgttaaa cagactgaaa ctaaacttat tatcccccaa
    17341 gttgccattt cattatactg tgatgtttta agccagatgg ttagcccttg ctcaaaacaa
    17401 atgccggcaa cacaacaaat tgacatcact actatgaatg ttgttcttat tattttactg
    17461 ctatagttga ggtatgtcga tttgtctgaa ttaaattgta aagcagttaa ggtgggttga
    17521 aacacggcat accctgtgcc ggtcataccc cgtaatagat aataaggttt tgtaataatt
    17581 gaataagttg taactgcacc aacatttaaa actgttccta taattaattt gtcggcctga
    17641 acagcaagga agcttattat cgcatggagg aacgccctga atgaatattg cgagactgat
    17701 gtgcgctttc tttcaatctt aaattgaagt tttgtgccca ccaaaatcat cttccttgaa
    17761 attaatgtga tatctataag cagggatatg atggatataa atacaatagc tatgctatat
    17821 aagataacgt cgtttattcc acatctccaa agtgctatca gtgcaatcag gtttaatgcc
    17881 tgacagatgt attgccaatt gttcctcaag ctgaaaaagt tattcgcttg taataattgt
    17941 tgcggaacag ctccaatcac caggaagaaa ctatttgctg cggctagtaa gaaaacgggt
    18001 aagcatattg tagccgtatt gcctgtaagt tgaaacagct gtttgccgta aaatcccatc
    18061 cctagaaaaa tgagcgaaat gcaaatagct gctaataccg atagcgtaat tgaagtgctc
    18121 agcgcgcgac taaattttat tgtttcttta tcttcaagaa attctactga aaaacgcatt
    18181 gctgatttag atgccccaaa atcaaatgca gtaaacgaag aagaaagccc gaacgccagg
    18241 aggaaaaggc catacgtgtc ggctccaaaa gctttcaggc aaattggaag cgtaagtatg
    18301 ccaattccta gtttcgctac atacaggaat agattacttg atagattttt aaaaacatta
    18361 gacatgccgg taaataacta tctattgatc aactttgtag aatataccca tcaataaact
    18421 ccctcaccgc cccctctcca ccgctttttt gtaaatgtat gattccggca atggctttca
    18481 ctttcggagt ggcattggcc gggcatgcag ctaagcccac tgcttgcaat agttcaatgc
    18541 aattaatatc gtctccgata taagcaactt catttagtgt aattccctcc ttggtgcaga
    18601 tttcttgtgc tgcagctagc tttcctccga atccaacacc ttgatataaa taatctactt
    18661 tgagtttatc ggcacgtcgt tttacgatgg gcgtgtcttc agttgtcagc attcctgttt
    18721 taatgcctgc tttttgtaac atcaccatgc ccattccatc atgcgtattg aattttttaa
    18781 attcatctcc tgaggcagta taatacatgc ccgcatctgt caatacgcca tcaacatcag
    18841 tcagaaatac ttttattttg tttttcatat cgatttattt taccaggcag tccaaccacc
    18901 atctactgtt aatacacttc cggtcatata gcttgacgca tcgctggcaa gaaataaaaa
    18961 tggtcctgtc atttcatctc ttcctgccat ccttcccatt ggcgaaagct tttcaaagtt
    19021 cacaacaaat gactcgctgt ggtgattgaa aatgccatgc ggcgctattg cattacatcg
    19081 gatgttatcc ttcgcataaa aagttgcgag ataccttgtc agattgggaa tcattgattt
    19141 ggttgccaca taatctaccg gtttataatg gatttcctct gaaccgaaat catacaaact
    19201 ttgatttggt gcaactaatg aatatgtgct cgctacatta ataatattgc cattttgttg
    19261 tttcagcatt tgccttacaa cagtttgagt gatgagaatt aatcccttga tgtttacctc
    19321 gtaagaacgg tctaattgtt caatagggaa gttttcaaag cgcgttgcat ttaatacatc
    19381 attggattgc gcatcgaatt tggcatcgat ggcggcatta ttaatcaaca catcaatatg
    19441 gtcaaaggcg ccaacggcat catctacgca tttggcacag gcacttgatg aagtaatatc
    19501 tgtgatacag attaaataat ctgcgccttc gcgaaagggt agaagatgtt cccgcatatc
    19561 ttcaatcttg gaagcattgt gatcacacaa aaccaatttt gctcctgatt caagtatagc
    19621 cttggataag gtttttccaa tgagtccaaa tgcacctgtt aaaagaatga ccttgttgtc
    19681 taatggaatt cttgtttgtt tcataggcac atttcaatta ttgtagctga atgtaaaatt
    19741 tggtcgaatg agtaagcgct gtgatctgtg ttagactgaa tgcgatcaaa gaaatggttt
    19801 aattcggcaa tgaataaatc atttctatca aagtcttcta aagtttcttt attggttgat
    19861 gtggctgtta ctgttgtaag tgttttagca aagaaatcca tttccataca accatcttca
    19921 aacacgcagc ggtatgtgcg acgaggtact cgatcgacat aattcatatg cacatgtgca
    19981 accacattat tcatatatct gatttgaaaa tctgcaatgc cctcaacatt tatgtccaga
    20041 tgctttggat attggtactg ctttgaaaat tgtgcaactg gagcattggc aagtcggttg
    20101 gccatgtcta aatcatgact tagtgtcaaa gctgctccgc cacctagatc ttttcgcgca
    20161 gcataacctt gtttatagtc ttcccaagga tgccagtcgg gtaaataact tccccaataa
    20221 gtgtctataa aaagtaattt tccaaatgat tcctcttcaa ttgctttttg cattttttgc
    20281 aaatgcggat gaaaagccat catataggct acttgcatca atttacccgt gcgtttacat
    20341 gtttccgcaa tttcagaaag ttgatgcgtt ttgtgcgaca aaggtttttc caccaacaca
    20401 tgcatgccct gttccagaca aaataatgtt tgctcggcat gtaaagatgt aggagtgcaa
    20461 ataatcgctg cttcaaatgg gatcgtctta atgtctgcta gcgatgtaat aattttgatt
    20521 ctggatgtat caattgttcg catgggtaaa ttgcgacttc tatacaccgt aatattctca
    20581 tagccaagcg aaagtaaatt tccgatatgt cgctctccaa tagagcctgc acctattact
    20641 aatattggtg ttcgattatc catgaaattt gatacaaggt tgcgctgcaa taattttttc
    20701 tgctttctcg aaactggcta aatcatcaat gtcaacagaa agtgatgcat caataatcat
    20761 tggaagaatt ttttttccgc tcatgcctcc tgcttctatt gttgaagttg caattacatc
    20821 cagtgtccct acttgccagt aaactttagg caattgctgc cgaggcatat taaatggctc
    20881 atcgatatta tttatgctaa gcagtggttc cattgcggca tgtttgtctg ctccaagtgt
    20941 ccacatttta tatggcgtat tcggtgcttc tgtgataatg cgcagcgaat cggcattgcg
    21001 ttcaactaca gcgcgaatac catcatctat ccaacccggc attcttatgg gtgaagtagg
    21061 ccttagctga acaataatat caggtcgata ttgctcattt tcaaggtgcc attttaattg
    21121 atgactaaaa acttcaaaat cagtggtatt atctagtgcg aggttagccg gtcgcataaa
    21181 tggaatttca gcaccatatt gttgtgctat tgcagcaatg tcaagatcat cagtattgac
    21241 aataatacgt gtaattaatt cactttgttg agccgccaaa atactatatg caagaagcgg
    21301 atgaccattg agtggacgaa tattcttccc tggtaatcct ttgcttcctc ctcttgccgg
    21361 aataatagcc agtacttcca tgtagtatta attacctgac ttaaatttta atttatttcg
    21421 ttgtgcttgt tcaacaggta atatgtcttc atacttgtat tgaagagctt cctgcatatg
    21481 atgtaagtcg cgcactaatt tttgcaatcc cggaacttcc agactcgcag cgtgatcagt
    21541 gcctttccag gtgcggtctt tggtaaagtg acgttctatc catcttgcac ccaatgtata
    21601 tgcagcaata tcggcggcta ttcctaaatg gtgaccgctg aaaccaattt cctttacgcg
    21661 ctcaccaaat tgctgatata atttattgat ctccagcaaa catatttctg aaaattgaat
    21721 cggatagccg cttgtgcaac tgtataaaac caatctgttc tttgcggaat tagtttgctc
    21781 aaaaaaggaa accatttttt cagtttcgtc tgatgtggtc atcccgacag atacatgaat
    21841 atcaccgtta taggtgtcgc gcaatatttc gagtaatgca aagtttgtgt tggaggctga
    21901 tgggattttt ataaaatcag gatttaatcc tatcaattca gaagcactgg taatatccca
    21961 caccgatgca ctatatccta tgcctatgct ctctgcataa gattttaaat gtgcgtgctg
    22021 ttcaaccgtg aattccagaa actcgcgatg ggcgccatag gtatcaccat agctttgcca
    22081 tggctccgga tgcggagcat tatattgatt gttgtccaat aattcctttg gatttcgttt
    22141 ttgaaatttc gcataatcag caccacactc ttttgcaagt cggatgagct cctttgcaat
    22201 atcaaaatct cccatgtggt tgcatcctat ttcagcaatc acttttggca ttcgataatc
    22261 aaattgaaac ataggtcatt ttacagcttc gcctcctcag tcacattggt gctaagtcga
    22321 ccatgaaaaa attacattta ttcggcaacg ccattcgacc ataatacaat ggacgaattc
    22381 aatatgccgt tttctttata tgaagcaaat gtttgctcat tcactactgt ggtgagcact
    22441 ttgcgtttaa ttaaagtttc agtttttaaa acccaatatt gcaatcgctc cggatccact
    22501 tcgccaacta tcatcaggtc gatattgccg gtatcctttc cttcagcata atctccggtg
    22561 atcaatgcat agtgtatttc accgatctgc ttaacaatat tttctacgat cttatcaatt
    22621 cccaaatact tcctcaccag actattcaat tccggaaaaa gcgggtgttt atcgtttgcc
    22681 ttgtattgaa tggtatttcc ttcaccatac gacactaaaa atccggcctt cgacagcttg
    22741 ttcagttcaa gtcgcactgc atttgtagat tcatgcatct ccttcgccaa cccccgtaaa
    22801 tgggcagtcg accgcgtatt gctgaaaaac ttcaacaata atttgatcct ggtcctcgat
    22861 gtgatcaacg attctaacac agaaacgggt aatgagtaac aaatttactc atttcaacaa
    22921 aaccaaaaaa aaatgtgaaa caccctcctc ccccctctct cctttttcct tcctcctctc
    22981 tcctttttcc tctctcctcc tcctcagaac aatctcattt gccccggtct tctaaacagc
    23041 gaagtattat aagtaaacat cttcctctcc ggcatatatt tatccctcga caacctgaaa
    23101 agtttgtgaa tagactccgc caaatgcccg tcgccacgca ttctcagctt ccatctggaa
    23161 tcgttgagtt gcccctggtg aactgccctg atgttgttca atacacgctc tgctttatca
    23221 ggaaaagcct tgtgtatcca atcgctgaag atttgaccaa ctgcaccatt caatcgaacc
    23281 atattgaatc ccgcatcgaa ggccccatgt tcagctgctg cagccagtag ggcaggaatc
    23341 tcgtgatcgt taagtcccgg tattacaggt ccgatcataa tacccacagg aatatgcttt
    23401 tcgcttagct ttttcagaat atccaagcgg ttggcagcag tggaagtgcg cggctccatt
    23461 gcccttcgca gcgattcatc cagcgtggtg acgctgatat atactttaat aaggtcatgc
    23521 tgtgccagct cctccagaat atccaaatca cgcagtatca aacgattttt tgtgataatt
    23581 cctgccggat tcctatatcg ttgacatacc tccagcaagc ccctggtgag ccgcaatttg
    23641 cgttctacag gctgataaca atcggtgttc cctgaaaaac ttacgggggc aggaacccag
    23701 tttttgtcat taaacgcctt ttccagcagt tttggggcat ccggcttggt gataatgcgt
    23761 gtctcgaaat ccaatccggc actaaaaccc cagtattgat gcgtgttgcg ggcataacaa
    23821 tacacgcaac catgctcgca accctgatat ggatttaccg aaattcccat gggtgaatcc
    23881 ggactgtcaa caaaattgac gatgcgttta gggtattctg taaaaaactg tgtttcgccc
    23941 ggtgcaggcc agctttcgtc aagaccttca atatgctcct gagcatagtt aatgtcgtga
    24001 taaggattaa aagggttgat ctgtgaaccc ctgccttgaa aaaatttttt ttcgtccatg
    24061 cttgcaaatt cgaaaaatgt cattattttt gacaaataaa gtcatatttg ctgacattca
    24121 caaaaaagaa aagcacatgt caaacatagc aagtaacctc aggcatctgc gcggagagcg
    24181 caaacttagt caacaagaat tggccgacat gataggaatc ggtcgaacca cgattgccaa
    24241 tattgaatcg aacctggcaa atccgggaca taaaactttg ttggagatcg ctaaagtttt
    24301 taatgtaacc atagacgagc tcctgcatgc cgatcttaca aaacgcattc ttattcagga
    24361 gaagccggct caacatgttg cattccgacc ggttgtagtt actgttgatg agctcggtca
    24421 ggaaaatatt gtgttggtag acaccaaggc agccgcaggt tatccaaccc gatatctcga
    24481 gcctgaatat tatcgcgaac taccggcatt taaattaccg ggtagcgatt atagaaatgg
    24541 aaccttccga tgctttcagg ttgatggcga tagtatgcag gataccattc aacacggcga
    24601 ttttgtggtg ggtagatttt gcgacaatca tttcaaagat gtgcgcgatg gttatatcca
    24661 tgtggttgtt actaatgata atgtcctggt gaaaagaatt atcagtcgcg cagaatctga
    24721 acataaatta tacctcatca gcgataatga tgcatatccg gaaatagtag tcgatattga
    24781 tgatgtgcgt gaaatatggt ttgtgaaaag taaaatttca agctatatgc cggtgaagaa
    24841 aaatgatctc gataaagtga tcggtgatct taccatgcaa gtgcacttac tgaaaagcag
    24901 aattgatcaa ctcgaaaaaa actaaatcaa ttagcaagtg gagcgcagcg acatcccgac
    24961 ccctcgggat tagcaagtta gttaaacaac acatcaacaa atcatacccg gaactacaag
    25021 agcggactgc tgctggaaaa aatatttggt acataatttt tcgctgcaac cgatcttccg
    25081 ccgggatttt taaataacaa agctgatacc gaatgacatc gctcaaaaga gccggtgcag
    25141 gggattgtcg gcctaaaaaa agaaaaatta tgatcgcacc aagtaacatt attaccaacc
    25201 gccaacgcat tgcaatcggc gaagaattga tctggcagca attgaaagaa aaaagagttt
    25261 ttggtttgac tttcgctagg caatatccat tttatttaca acaaccggat ggtgagcgat
    25321 tggtatactc agcagatttt tattgtcatg atgcaggtgt tgcagtacgc atagttaact
    25381 gcgatgccat taccgctgag tataatgagc aaatggcctg gtgtaaacaa caattgcaaa
    25441 tgcgcggaat tttggtaatt gaattgccat gcgacgccat tgcagatctt aaacaggtta
    25501 ttgctgtaat tcgacgagaa ttaaaatata agacgataca cgcatgaaaa tctgtagttg
    25561 ctttccggag taatttcgat actgcttatc aatttaagag ctttcaacca ataggtaatt
    25621 acctatcttt gtgaaatgct acctgtattt actaatccgg ttcagattac tcctgagata
    25681 cttgtcggga acggcaaacc acttcttatt gccggaccat gtgttattga aagtaaagcc
    25741 ctttgtctcc aggtggcaga aaccatgaaa gaggtggcag cgcgtaccgg gtttcaatat
    25801 gttttcaaag caagtttcga caaggccaac agaacagcag gcggctcctt tcgaggtgat
    25861 ggaatggagg ccgggcttga aatactgcaa catatcaagg aaactgtcgg cgttcctgtg
    25921 ctgacagata ttcatcttcc cgatcaggca gcaattgttg cccaagtagt tgatgtcctc
    25981 caaattccgg cattcctctg ccgccaaacc gatcttctta ttgccgctgg tgcaacaggt
    26041 aaagcggtga atgtaaaaaa aggtcagttt atgtcgcctg aggatatggc ttatgccgta
    26101 gagaaagtgg catcaacagg aaatgcaaat gcactcctca ccgaaagagg ggcctccttc
    26161 ggctaccata atctcgttgt tgatatgcgc tcattgccaa tcatgcgaca atatgcgccc
    26221 gttgttttcg acgtcaccca ttcagttcaa atgcccggag gtgccggagg aaaaagtggc
    26281 ggcaaacgcg aatttgctcc ataccttgcc aaagctgctg ccgcaaccgg cgttgatggc
    26341 ttcttcatcg aaacccatcc caaccccgac caagccctca gcgacggtcc caatatgatt
    26401 cctctccatg aaatggaaaa cctcctcagg actttgaagg cgattattga tatcgtaggg
    26461 tagctatcgg gcgttagtat ctagagccaa gctcgcctcg cctccggcgg gtatctagta
    26521 tctagacttg gtatcagg
//
LOCUS       DAOQOU010000027        26357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_853_length_26357_cov_6.302943, whole genome shotgun sequence.
ACCESSION   DAOQOU010000027 DAOQOU010000000
VERSION     DAOQOU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26357
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_853_length_26357_cov_6.302943"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLM90_04065"
     CDS             <1..467
                     /locus_tag="PLM90_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ88574.1"
                     /translation="TMHAQMGWRLLLNEHVHIERSGNKIAVAGVENWSARGRFKSYGR
                     LEQTLHGIQDAMTVLLLSHDPSHWEGEVLDHPAKVDITFSGHTHGMQFGLDLPWFKWS
                     PVKYLYRQWAGLYQQNDQYLYVNRGFGVIGYPGRVGILPEITLFTLQTTQSE"
     gene            464..991
                     /locus_tag="PLM90_04070"
     CDS             464..991
                     /locus_tag="PLM90_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ88575.1"
                     /translation="MIYSRYQQKLNRYETIVNELFLAAERQSASALNTPLQEGKWSTG
                     QQLFHVIRVSEVTIDYLEYKIQQQAAAPKAGISSFVRSTLLNIALILPIKFKAPAITA
                     AVGEDHTISDLTGYHQQQIIRIKAYLGQMTAADRKKALFKHPRAGRISMDQTLRFLAL
                     HARHHLKQVNKGINI"
     gene            1056..1796
                     /locus_tag="PLM90_04075"
     CDS             1056..1796
                     /locus_tag="PLM90_04075"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMZ88576.1"
                     /translation="MTIFFVFFAIHWYLSLFSQTFFLHRYAAHSMFRMNPFWEKSFYL
                     FTWITQGSSYLSPYAYGILHRLHHAFADTEQDPHSPKYTKGIFPLMHKTKQIYSDINS
                     DTFPVDEKYKKNLPMWRSFDHFASSMIVRLLWVAAYFVFYLTFATHWWMWLLFPITVL
                     MGPVHGAIINYFAHKFGYRNFQVEDTSKNFLPVDVLMMGESYHNNHHKFGSRPNFGVR
                     WFELDPTYPLIKILHALHIIRLNKETAA"
     gene            1951..5310
                     /locus_tag="PLM90_04080"
     CDS             1951..5310
                     /locus_tag="PLM90_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88577.1"
                     /translation="MPGDTLLLDTLSIVPGSVIIQSLSIQSADTLYTVLDTNQFTISH
                     FNATIYFHPAPLGSICISYQVFPYLFTKKTYRYDSNAYDSTALNLSPYTIATNKQDVW
                     SAMSGLDYSGSFARGISFGNAQDVVVNSTFNLQLSGKLQNDVDISASITDNNIPIQPE
                     GNTQQLQEFDKVFIRLSKGGQQLTMGDFEISSKEGYFLQYYKKLQGASYSGNFTLNNG
                     STLQTGISAAVARGVYARQEISVIEGTQGPYKLKGNNGETFIIVLAGTERVYMDGSLL
                     VRGSEHDYVIDYNSGEVTFTPKVLLTKDKRVVVEFEYSDKNYFRTLVYSGTTWTSTDE
                     RWKTRLQIYNEQDSKNQPLDQTLDADDRQALEAAGDSISTIYAPGIDSIAFDASRILY
                     RMVDSLGYDSVFVYSTDPDQAFYALAFTELGSGNGNYIEATTTTNGKVYQWVAPIAGV
                     PQGSAEPVILLIAPGKSRLITLGGEFNPDKKTKITGEFAISDNDLNTFSPTGNNDNKG
                     IAVRTGVSKAITSSTNNSLTIFANYEYAQSTFKTPERYRPVEFNRDWNIYSTEASDEH
                     YANAGVEWKPSDDISVGYTTSTFIRGLQYTGFLQDLHTQKTGKSWQWDAKAGLLTAAT
                     DSNNSIFFRPRINISKTFQQLNYVKTGIGYEGEHNAIKSIDDSLLATSFYYDQYNWFI
                     SNADTSKRYLRGEVIYRKDDAVFENKFRPVTEGITYQLQGRIGVHPQRSWQWQVYYRT
                     LSISDTSLTTQDTEQSLLGRLQQSSSFKKGLLTTDLSYEVGTGREPKRLYTYVETEPG
                     AGTFTWNDYNENGVQELNEFEIAVFADEANFIQVLIPTDDYIQTNSTAFNYSLGINPR
                     VLWNNALGLEGMLGKCAVQSNLQMNRKVFDDQTISVYNPFASIDDTLLVSSNIYWVNT
                     LFINRSGSVLGADYSYNRNYNKQVITIGPESRGKEEHVINIRYRPWKPLITNITLKGG
                     VKDYYSPVFTEKNYGIDFREITPKITYNASNIFRTTLSFTYNESANGEGFEALISREA
                     SIDMKYSVVSKNTIQLKFSFVNNDFNGDADSPIGFAMLEGLQNGNNMIWSLNIDRKIS
                     QILQLNLGYEGRKTGDNDITHIGRVQMRAVF"
     gene            5362..5745
                     /locus_tag="PLM90_04085"
     CDS             5362..5745
                     /locus_tag="PLM90_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ88578.1"
                     /translation="MKASTNSINWFEISVADINRAASFYSTIFDITMDRQEMMGMHMA
                     FFPWENGSGKANGALVQGDMYVPSADGVKLYLNADPDLQVVLDRIQGAGGQIVMAKTA
                     IGDGLGYMAFFVDTEGNVVGLHSNA"
     gene            5760..6194
                     /locus_tag="PLM90_04090"
     CDS             5760..6194
                     /locus_tag="PLM90_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HMZ88579.1"
                     /translation="MDITINTPALLFPAISLVMLAYTNRFMALSSVVRKLHDEYEANR
                     SNKNLPLQIRNLQFRIKLVRRMQFFGVTSFIVALISMYLIYVDNLQMAHITFATAMLL
                     FACSLVISLIEIMKSTNALELELSDMEDEEGKSFVDYIKKHI"
     gene            complement(6406..6777)
                     /locus_tag="PLM90_04095"
     CDS             complement(6406..6777)
                     /locus_tag="PLM90_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88580.1"
                     /translation="MKNLCTLLIAIALLCGTANAQSIPDGTYVYTGLASNGHPLTFTT
                     TVSPTGLTFSEAIDGGVLCSANMVVESIDPSGTIFTYGTTTPGAADCTLGQDFIFMLT
                     PTPSILEFRKGEGPVFTLNLQ"
     gene            6945..9545
                     /gene="mutS"
                     /locus_tag="PLM90_04100"
     CDS             6945..9545
                     /gene="mutS"
                     /locus_tag="PLM90_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMZ88581.1"
                     /translation="MKAVKETPLMGQYNRIKAKYPDAILLFRVGDFYETFSQDAVVTS
                     KVLGIVLTKRANGSASHIELAGFPYHALDAYLPKLVRAGYRVAICEQLEDPKLVKTIV
                     KRGVTELITPGVATNDKMLDTRSNNYLASYYGDKQMAGMSFLDISTGEFFVAQGGADY
                     FEKLLHSLQPAEIILPKQHQKAFKELYGNKFYIYAIDDWVYTEDYTKEKLLNHFGTIN
                     LKGFGIDDMPFGIMAAGASIHYLSDTEHNNIGHITGISRLSEEQSVWLDRFTIRNLEL
                     IHASSEDGSSLLKVLDHCITPMGTRLLKKYILMPLKDKHGIDARLDMVDHLLHDNELA
                     GVITNALKQIGDLERVVSKVSLQKINPREILQLNRALHACAPLRQALMQSNQPTLQKL
                     GDQIHLCELIRDKIDREIAEDAPAQVHKGGLIKQGVHAELDELRAIGSSGKDFLLRLQ
                     QDEIIRTGIPSLKIGFNNVFGYYLEVTNKYKDVVPSDWIRKQTLTNGERFITPLLKEY
                     EEKILGAEEKILALEQQIFDTLLSELQDYVRPIQINANVAARLDVLLNFATIANEYRY
                     QKPEITEDFGIDIELGRHPVIEHHLPIGEGYVPNSISLDTEEQQIVIITGPNMAGKSA
                     LLRQTALIVLMAQMGCYVPAQRARIGLIDKIFTRVGASDNISSGESTFMVEMNETASI
                     MNNLSARSLILLDEIGRGTSTYDGISIAWSLCEFLHQHPHARAKTLFATHYHELIELE
                     QKFDRIRNYHVSTREAGNKVIFLRTLQQGGSKHSFGIHVARMAGMPPQIVSRANEILK
                     QLEDKHIGVDLKNKLKKMQSQQFQLNMFSVDDPQMAKIRALLEETDINTLSPIEALMK
                     LKELRDLTGE"
     gene            complement(9573..10322)
                     /locus_tag="PLM90_04105"
     CDS             complement(9573..10322)
                     /locus_tag="PLM90_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HMZ88582.1"
                     /translation="MRTLGLILLMMLVQHNFAQVINIEDKRIKTDTVGWSGSGELSLY
                     ANKNNDMVLSLQTDLHLQYKTKKSLWLLLTDIATVQVNNEQHFVNSGFQHFRYNYKIR
                     DRFVWEAFAQGQYNEPLAIDYRLLAGTGPRFKLYGTDTFRCYVAALYMYEYEQNTGEI
                     DAQQNNRLSSYVSFTYSPSDHFSLVSTTYYQPKLDDYTDRRISTSLDVKSYITKKLYL
                     KLNYNLLDDSAPAEGVVNTIYEFTAGMGFDF"
     gene            complement(10349..13468)
                     /locus_tag="PLM90_04110"
     CDS             complement(10349..13468)
                     /locus_tag="PLM90_04110"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88583.1"
                     /translation="MRKSLLYLLLLSTISSFGQKNKQNSNLSLPFDTAITQHLNFRNV
                     GPWRGGRSTAVCGDPDDPQVFYFGATGGGVWKTQDGGNNWKNISDGYFGGSVGAISVS
                     KSDPTVIYVGLGENSMRGNVSEGTGIWKSMNSGRTWTYLGLPETRHIMRIVIHPKNPD
                     IVYVAAQGHLFGPNTDRGVFKTIDGGLHWQKVCYVDDKTGACDLVMDPGDPNILIAGF
                     WQVIRTPYSLESGGPGSGIYKSTDAGATWQNISHASGLPKDTLGIVGLAIAPSDPDIY
                     YAIIESKTGGVFRSEDAGKTWTKTSDNSNLRQRAWYFSKIYVDPKQSETVYVLNVEFW
                     KSTNGARDFKSIPTPHGDHHGLWIDDRDPQRMIVADDGGAQVSFNGGNEWSSYYNQPT
                     AQIYRVSTDNAVPYRILGAQQDNSSLRIRHRGTYGQIGNNDWQPTAGFESGYIVADPL
                     NPDIVYGGNYSGYIGCLNHDTRDTRNITVWPDDPIGQGAGDQAYRFQWNFPLFFSPHD
                     PNRLYAAGNVLFYTDDAGTSWHAMSPDLTTNTKSMQQPSGGIITKDNTGVEVYCTIFA
                     ASESGLEKGVIYTGSDDGLVYVTKNNGSDWQNITPKDLPPYTMINCIETDPIQKGKMY
                     FAATRYKLDDEQPLLYVTEDYGVTWKKIINGISPTHFTRVIRADRNREGLLYCGTERG
                     LYISYNGGNNWYPFQLNLPIVPITDMTIKDNDLIIATQGRGFWVLDNITMLQQMQAVN
                     NAFISYPVRNVYRMSMYKTEKPVNAGENPEPGVVFDMFIPAAKAKSGADSLSKCSIEI
                     KDHEGKSITTLATDAKEKSNTINIKPGFNRISWDMHYPGGKSIDGMFLWNGVPDGPLA
                     APGNYTAEINYGNTTTTQSFFIEPDPNSTATLDDYRLQHVFLMEMLETFNSTQEAILD
                     MQEMQKQMRDYLTKLDSLAPQSLKDSIANINKDLDAIINTLYETRNKSSQDMLNYGIK
                     LNDKLSGVIGAVSTGNFRPGNNQRMVYYDLKKQIDMQLEFYRDIKKNRISTLNKTINN
                     LALPVIITK"
     gene            13576..13663
                     /locus_tag="PLM90_04115"
     tRNA            13576..13663
                     /locus_tag="PLM90_04115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13610..13612,aa:Ser,seq:tga)
     assembly_gap    13951..14050
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14481..15116)
                     /locus_tag="PLM90_04120"
     CDS             complement(14481..15116)
                     /locus_tag="PLM90_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88584.1"
                     /translation="MKAFLRIFLILVVILLILAAAFIAKYPFYAPWYRAAHSAEDYKL
                     LVESTTKPESGVTLIQAFNDRYPYWLGTRWNFNGISEVPGSGSIACGYFVTTMMRDMH
                     FDINRVKLAQCASEEMIKALVDEKHIKRYNLVESDLMFDNIRKQKQNLYIIGLDNHTG
                     FVYNDGDDIWFIHSSGSFPYCVMKEDATACNTITDSRYKVLGCISQSDILF"
     gene            15196..15624
                     /locus_tag="PLM90_04125"
     CDS             15196..15624
                     /locus_tag="PLM90_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88585.1"
                     /translation="MADIKTINNSSDIMHCFEVIHTLRPHLNEEQTIACFEQMRSEGY
                     HAVYVEDNGKAMAYCGYRFITHYFSGSAMYIDDLVTVADARNKGYASLLLDHVIEIGN
                     KKGLETIRLDSGHHRHDAHRLYLNKGFHIHSHNFSMTLKK"
     gene            complement(15626..17002)
                     /locus_tag="PLM90_04130"
     CDS             complement(15626..17002)
                     /locus_tag="PLM90_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ88586.1"
                     /translation="MQYRLSFRHIIIIIGLSAIFASCNQDGNIFPTLDDQYDQYGSPF
                     DSVPETGNIVMYEINLRAYSAAGNIQSIIADLDRLKDISVNTIWLMPIYDEGELMAVH
                     SPYCIRDYYQVSDEYGSLQDLQQLTDAAHARNIAVVLDWVANHTSWDNDWITDHPDWY
                     TQDADGNIIHPPGTTWTDVADLNFDNQDMRAQMIDAMKFWVLVGNVDGFRCDYADGVP
                     FDFWQQALDSLRNIPDRTLIMLAEGDRDDHYTAGFDMTYAWDFYGTMQNVYSGSAVSD
                     LYATNNAEYAGVPAGDKKLRFTTNHDESAWNATPMVLFNGIDGAVAASVMTIFLNGVP
                     LIYTGQETGNTANVPFFSNAPINWTLNPEMQLEYKQILNAYSGHQTARTGTLTTFSNA
                     DVYCIKKTLASDQFVVLVNARSADVTYTLPTALKDTEWTDAINGNSVSLGTELALTPY
                     QYLLLTNE"
     gene            complement(17024..18106)
                     /locus_tag="PLM90_04135"
     CDS             complement(17024..18106)
                     /locus_tag="PLM90_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ88587.1"
                     /translation="MRIGFDAKRIFQNATGLGNYSRAVVKNLASQFPENEYFLFTPNA
                     KDQLSFNAEGNIKTVIGRGSVWRSFGIRRDILKHKIDVFHGLSNELPFSINKADVKSV
                     VTIHDLIFDLFPHHYPGVDRTVYELKTKFAVNNADVVIAASESTRNDLVRMYGKDPAG
                     ISVVYQSCDDIFYAPPAADISSYRLPKQYMLYVGSVTERKNLLAVCNAYTKLPSADRL
                     PCVIVGNGKRYADEVRAFVSANHLTSSFIFLENVPTDHLPALYANALCFIYPSLYEGF
                     GIPILESMVSGCPVITSSVSSLPEVGGDAALYFNPENPEDIAAQIQRVVGDEQLRQNM
                     INKGKLHAQQFEKSKVTRQLMSIYKQ"
     gene            complement(18207..20318)
                     /locus_tag="PLM90_04140"
     CDS             complement(18207..20318)
                     /locus_tag="PLM90_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HMZ88588.1"
                     /translation="MLNLCTPRIISALFLSASIAAATVSYAAPLMVGEDCNTAVSITE
                     GTYTAPNPDYWYSFTVPTTGTYVISTCDMGNTCDTRLYVYDHCVGLIPTELAEGTLSY
                     NDDYCGSQSQITTLLEAGDLVYIRIGDAETACAGESINWSIAYTGLPTGCIDAFACNY
                     SPIAMVDDGSCIYPGNPDCPSGPDLVLDETYFDGDISTGWGTDFQLSTINADDWTNSC
                     YIDEGSLTGPGMRTIIRFGIKIWNLGDEDYHIGTAAENPYLVFDPCHGHTHYVDYGEY
                     LLYDSLGTEIPVGHKNGFAVMDLCGIGGYNGADMGISAGCFDAYGLGTGGQWIDITDV
                     PDGTYTFVARVNWENHPDIEGRVETNLANNWRSRCIHIWRDAGVINAEMIDDCPLYID
                     CLGEIWGTAQLDCEGNCNGWHHIGDLNEDSTRLYDDVDLYVNNILSNTIIADMCNDAN
                     NDSDIDVVDAALVMGCANQTYGYTHDTDLCELPVAMMNTSDTVTYSIGTVNAPFGYLD
                     IYSLNPSARVLGAQFTMEGITIDSVANLCPEVFGENYMLTYTDNEVIALGHNEVPYQI
                     HFEPIPTFRIFYTATYPTEICIKHFTAAVNEDFEEVVTALDNSCVTVDETGIQYYASN
                     PLEISIQPNPFSTAAELHFSNAGHQVFDLVITDINGNVVRTLNTTGNTITINRDELPA
                     GMYLYRLTGISESQSGKFVIN"
     gene            complement(20407..20772)
                     /locus_tag="PLM90_04145"
     CDS             complement(20407..20772)
                     /locus_tag="PLM90_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HMZ88589.1"
                     /translation="MSLELFYLASCSTCQRIMKELKVQKYPFRLQDIKTEQITPGQLD
                     RMKDLAGSYEQLFSRVALKYRALGLNQKKLSEKDYKKYILEEYTFLRRPVFIIGEKIF
                     IGNAPSTVAAVAKELTALK"
     gene            complement(20803..21765)
                     /locus_tag="PLM90_04150"
     CDS             complement(20803..21765)
                     /locus_tag="PLM90_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HMZ88590.1"
                     /translation="MKIIPSNIRRLVANKYARVVNFTKRTSFPGFAGIPIYDVISFFW
                     QEIRRDQLPVRASAISFNFLLAIFPSIIFVFTLIPYIPIKGLDANIHDFFRDVLPESG
                     YAFLESTITGITSIKRGGLLSVGFILAFYFSTNGVRMLMLTFNKSHPIYQRRNFWRNR
                     IAAFRLTFYLFLLFIVSIITIVMGDKVVGVVGDWINWEDNTGVFFLNIFQYAIILILF
                     FFGISLIYYYGPAVKHRWRFITPGGTFATIVSIAASVIFGYAANNLIGYNEVYGSIGT
                     LIVLMIWINLNALVLLVGFEINNSVDVNRVLRSRDGEDEQHGTV"
     gene            complement(21796..23700)
                     /locus_tag="PLM90_04155"
     CDS             complement(21796..23700)
                     /locus_tag="PLM90_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ88591.1"
                     /translation="MRTGIAIAIVCAFVSMLTFCTNNKGEATENVYLNANDTVSYVGM
                     ETCKLCHADKHATFIHTGMGMSFDKATKSKSAALFDAEHALVYDSTNNFYYKPFWRND
                     TLQIMEFRLDGQDTIHKRIEPIAFIIGSGQHTNSHMLQVNGYVYQAPITFYTQKQQWD
                     LAPGMSGGFNSRFSRVIEAECMTCHNGLPELVEGSVNKYAKVPSGIDCERCHGPGSLH
                     VARVSNGELVDTSKNIDYSIVNPRKLSVELQNQLCMRCHLQGVNVLNEGASFFDFKPG
                     DHINEHWNIFLPRFDGSNNKFLMASQADRLLQSKCYTSTHQLSCITCHNPHITVKDTP
                     REQFNAPCKQCHGEGNTQCKASIASRNNVADDCSSCHIRKSGSIDIPHVSISDHIISI
                     PKNTASKGSGTFKGLVCLTDSNPDPLTMARGYLHYYESFNKEKSLLDSAWQYIQKAGK
                     ENDEVRSVKVHYYYLKDAPDQIIQSGEKIKKDKLNAWTAYRMGEAYTAKSDFAAALKY
                     FNIAVEREPLNLDYLFKQATAMVFSGDNKSGEAVYRKIVGENPEYEKAWNNLGVILLT
                     KGQMPEAEQCLLKAIALNPDYTLSRIKLTELYIQSRQKSKAKSSLEYLQRYDADDPAV
                     ILLAQKLRSI"
     gene            complement(23743..24144)
                     /locus_tag="PLM90_04160"
     CDS             complement(23743..24144)
                     /locus_tag="PLM90_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HMZ88592.1"
                     /translation="MTNQPIIFFDGVCNFCNASVNVILRSDKHAYFKFASLQSEFAKR
                     FFEEHPIAPNIDSIILWEDGKLFYETTALLRVAGKLAFPWKLFVAGWIIPRFLRDPLY
                     RFVAKRRYRWFGKRESCMIPTPDIAARFLDS"
     gene            complement(24141..24443)
                     /locus_tag="PLM90_04165"
     CDS             complement(24141..24443)
                     /locus_tag="PLM90_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ88593.1"
                     /translation="MDISVEELKKRMDAGEDLLIIDVREPHEYAEFNIGAINIPLTSL
                     PMKMYDFDDDKDREIIVHCRSGMRSASAKALMVQSGFTNVRNLLGGMLEWQATVGK"
     gene            complement(24464..>26357)
                     /locus_tag="PLM90_04170"
     CDS             complement(24464..>26357)
                     /locus_tag="PLM90_04170"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88594.1"
                     /translation="AALSCYLPIQLQAQEILLPLKNNTALMQREVVAGSRSIVENRDT
                     LCMPFMDDFSAAQIFVDGAFTECGDSIIQTSSSIYPSSIFWVDSNAFVNRTYPSLPPT
                     YGVATLDGLNKYGQPYNETSTLGPADMLTSKPIFVGGAITDSVYLSFYYQPGGFGEYP
                     NLNDSLLLDFKAEDGTWSNVWFTVNTLGDDPQSFKLVMIPVADAYHYDGFQFRFRNWA
                     GINGNNDHWHLDYVYLDDDRTYNDTLFRDVAIIYQPEPYLRRYRQMPWNQFKDHQAEE
                     ISATHGVFMYNNFNTIVNTSHAYSVVEKYSGTEVMPLSAPVSVNLDPFTIAFDSYPNF
                     EIPEATPGYNDDSLSITFKYQLEPSGDINHRNDTLLHDQSFYNYFAYDDGSAELAYQL
                     IGLGSKLAMRFYANEPDTIKEIYIHWAYLDGSNSNKFFSLLLWDNIDTTGATADENII
                     YQNDFLTPKYIDSINGFYVYKLVDFLGNPAPVVVDGYFYVGWLQSQEASLDIGFDVNS
                     DAKENVFYNVGGTWQQSALSGAIMLRPQLGGNYSMYAPVENESPGTSSLMVYPNPATD
                     ILNINTATEEMQSIEIFDVDGRRVLVSDITSGTINIADLSAGIYILKILDAQKAMLAS
                     TTFVKQ"
BASE COUNT         7957 a         5361 c         5340 g         7599 t
ORIGIN      
        1 aaaccatgca tgcgcaaatg ggttggcgat tgctcttaaa tgaacacgtg catatcgagc
       61 gatcaggaaa taaaattgcg gtagcaggag tagaaaactg gagtgccagg ggtaggttta
      121 aaagttatgg cagattagaa cagacattgc atggtattca ggatgccatg acggtattat
      181 tgttatcgca tgatccgtcg cattgggagg gtgaagtatt ggaccatcct gccaaagttg
      241 acattacctt ttcgggacat acccatggta tgcaatttgg gcttgatctt ccatggttta
      301 aatggagtcc ggtgaagtat ctctatcgtc agtgggccgg attgtatcaa cagaatgatc
      361 aatatttata tgtaaatcga ggttttggtg taataggtta tccgggcaga gttggaattt
      421 taccggaaat aaccttattt acattgcaaa ctacacaaag cgaatgattt attcccgcta
      481 ccaacaaaaa ttaaatcgct atgaaactat agtgaacgaa ttatttctgg cagcagaaag
      541 gcaatctgca tcagcgttga atacaccgtt acaggaagga aaatggagta ccggccagca
      601 gctatttcat gttatacgtg tctcggaagt taccatagat tacctggagt acaaaattca
      661 acaacaggca gctgcgccaa aggccggaat aagtagtttt gtaaggagca ctttattgaa
      721 tattgcattg attcttccta taaaattcaa agcgccggca ataactgctg ctgtaggaga
      781 agatcatact atttcagatt tgacgggtta ccatcagcaa caaatcattc gaattaaagc
      841 atatctggga caaatgactg cagctgatcg caagaaagcg ttgttcaagc atccgcgagc
      901 gggcagaatc tcaatggatc agaccttgcg gtttttggcc ttacatgccc gtcatcattt
      961 gaaacaagta aacaagggga tcaacatttg agtaaacctc tgtttttaat acaaatgccg
     1021 taattttacg cccgatttca cttatcgatc attacatgac catctttttt gtgttttttg
     1081 cgatacattg gtatctgtca ctattcagcc aaacattttt tctgcataga tatgccgcgc
     1141 acagcatgtt tagaatgaat ccgttctggg aaaagtcttt ctatctgttc acatggataa
     1201 cacaggggtc gagttatttg agtccgtatg cttatggcat tttacatcgt ttgcatcacg
     1261 cctttgcaga tactgaacaa gatccgcatt cgccaaaata tactaaaggc attttcccgt
     1321 tgatgcataa aacaaagcag atttatagcg atatcaattc agataccttt ccggttgatg
     1381 agaagtataa aaagaatctt cctatgtggc gcagttttga tcattttgcc agtagcatga
     1441 tcgtgcgttt attgtgggtt gccgcttact ttgtttttta tctgacattt gccacacatt
     1501 ggtggatgtg gttattattt cccattaccg ttttgatggg tccagtgcat ggtgctatca
     1561 tcaactattt tgcacacaaa tttggttatc gcaattttca ggttgaggat acctcaaaaa
     1621 atttcctccc ggtagatgta ttgatgatgg gtgaaagcta ccataacaat caccataaat
     1681 tcgggtcacg accaaacttt ggagtacgtt ggtttgagtt ggatccaacc tatccgttga
     1741 ttaaaatttt gcacgccctc cacattattc gcttaaacaa agaaaccgct gcctaggcat
     1801 gcaacattct ttcgggtaga ttcgttgtaa atttacctga aaatcaggct tatccgtcgc
     1861 cttacagcta tactatgtat cttttgcgga ctgtttttcc gacagcccct gcatgctcaa
     1921 tggtccaatg agcgggtgcg gtatattgac atgccgggag atacgctact tctggataca
     1981 ttgagtattg ttcccggcag tgtaatcata caatcattaa gcatacaatc cgccgatacg
     2041 ctttatactg ttttagatac taatcaattt accatttcac attttaatgc tacaatttat
     2101 tttcatcctg cacccttagg atccatatgc attagttatc aggtattccc ttatttattt
     2161 acgaaaaaaa cttatcgtta cgacagcaat gcttatgata gcacggcgct caatttatcg
     2221 ccctacacta ttgctaccaa caaacaagat gtatggtcag caatgtcggg attagattat
     2281 tcaggaagtt ttgcaagagg gatatcattt ggcaacgcac aagatgtggt agtaaattca
     2341 acctttaatt tacagttgtc agggaaattg caaaatgatg ttgacatcag tgcatcaatt
     2401 acagataata atattccaat tcagcctgaa ggcaatacgc aacaattgca agaattcgat
     2461 aaagttttca tacgattatc caaaggtggt caacaactta ccatgggtga ttttgagatc
     2521 agtagtaaag agggctattt tttacaatat tataaaaagc tgcaaggagc atcctactcc
     2581 ggaaatttca cgctcaataa cggtagtaca ttgcaaacag gcataagtgc agctgttgca
     2641 cgtggagttt atgcgcgaca agaaatcagc gtgattgaag gcacacaagg gccttataag
     2701 ttgaagggaa ataacggcga aacttttatc attgtgttag cagggactga gcgtgtgtat
     2761 atggatggca gtttgttggt gcgaggttca gaacatgatt atgtaattga ttataattcg
     2821 ggtgaagtaa cttttactcc gaaagtatta cttacaaaag acaagcgcgt agttgttgaa
     2881 tttgaatatt ccgacaaaaa ttatttcagg acattggtat attcaggaac cacatggaca
     2941 agtacagacg agcgttggaa aacacgctta caaatttata atgagcagga cagcaaaaat
     3001 caaccacttg atcaaactct ggatgccgat gaccgacaag cactggaagc tgcaggcgat
     3061 agtatatcta ctatctatgc cccgggaatc gacagtatag cttttgatgc ttcgcgcatt
     3121 ttatatcgca tggttgacag cttgggttat gacagtgttt ttgtttattc aaccgatccc
     3181 gatcaagcgt tttatgcact ggcatttacc gaattaggct caggcaatgg aaactatatt
     3241 gaagcaacta caactacaaa tggaaaggtg tatcaatggg ttgcaccgat tgccggcgtg
     3301 ccacaagggt ctgcagagcc cgttattttg ctgatagctc cgggcaaatc aagattaatt
     3361 acgttaggag gagaatttaa tcccgacaag aaaacaaaga tcaccggtga atttgccatt
     3421 tcagataatg atttaaatac gttttcgccg acgggaaata atgacaataa aggtattgcc
     3481 gtgagaacag gtgttagcaa ggcaatcact tcatcaacaa ataattcttt gaccattttt
     3541 gccaattatg aatatgcaca gagcaccttt aaaacacctg aaagatacag gcctgtagaa
     3601 tttaacagag actggaatat ctatagcaca gaagcttcag atgaacatta tgcaaatgcc
     3661 ggcgtcgaat ggaaaccaag tgatgatatt tcggtaggct atactacatc gacatttata
     3721 cgcggactgc agtataccgg attccttcag gatttacaca cacaaaaaac cggcaaatcc
     3781 tggcaatggg atgcgaaagc agggttactg actgcggcaa cagatagtaa taacagcatt
     3841 ttctttcgcc cacggattaa tatcagcaaa acttttcagc aacttaatta tgtcaaaaca
     3901 ggcatcggat atgaaggtga acataatgcc atcaaaagta ttgacgatag tttactggca
     3961 acttcatttt attacgatca atacaattgg ttcatctcaa acgcagatac gtcgaaacga
     4021 tatttgcgcg gtgaagtgat ttatcgcaag gatgatgccg tatttgaaaa taaattcaga
     4081 ccggtaaccg aaggaataac ctatcaactt cagggaagaa ttggtgttca cccacaacgt
     4141 tcttggcagt ggcaggttta ttacagaacc ttaagtattt cagataccag tctaaccacg
     4201 caagatacag agcaatcatt gcttggtcga ttgcaacaaa gcagttcatt taaaaaggga
     4261 ttacttacca ccgatttatc atacgaagta ggaaccggca gagagccaaa acgcctgtat
     4321 acttatgtag aaacagaacc cggcgcagga acatttacct ggaacgacta caatgaaaat
     4381 ggtgtgcagg aattgaatga gtttgagatt gcagtatttg ccgatgaagc caattttatt
     4441 caagtattaa ttccaaccga cgattacatt caaacgaata gcacggcatt taattattcg
     4501 ttgggaataa atccgcgtgt cctatggaac aatgcacttg gattagaagg catgcttggg
     4561 aaatgcgctg ttcagtctaa tttacaaatg aacagaaaag tattcgacga tcaaacaatt
     4621 tcggtatata atccttttgc aagcattgat gacacattgc tggtgagttc aaatatatat
     4681 tgggtcaata ccttatttat taatcgcagt ggtagtgttt tgggggcgga ttattcatac
     4741 aatcgcaatt ataataaaca agtaattact ataggtccgg aaagtcgagg gaaggaagag
     4801 cacgtaatca acataagata ccgaccttgg aaaccattga taacgaatat cacattgaag
     4861 ggaggggtaa aagattatta cagtcctgtt ttcacagaga aaaattatgg aatagatttt
     4921 cgcgagatca caccaaagat tacatacaat gcaagtaata ttttcaggac tacactctcc
     4981 tttacctata atgaaagcgc taacggagag ggatttgaag cattgatcag tcgggaagcg
     5041 agcattgata tgaaatatag tgtagtaagt aaaaacacga ttcaattaaa attcagtttt
     5101 gtaaataatg atttcaatgg cgatgcagat tcacccatcg gttttgccat gttggaggga
     5161 ttgcagaatg gtaacaatat gatatggagt ttaaatatag atcgtaaaat atcgcagatc
     5221 ttgcaattga acctaggcta tgaaggtcgc aaaacggggg ataacgacat cacacatatt
     5281 ggaagggttc aaatgcgtgc agttttctga caaattttgt ttagggaaaa ttgcctactt
     5341 tagactcata aattaaatca aatgaaggca tcaacaaatt caatcaattg gttcgagata
     5401 tcagtagcag acatcaaccg agccgcaagt ttttattcaa ctattttcga catcaccatg
     5461 gacaggcagg aaatgatggg tatgcatatg gcctttttcc cttgggagaa tggcagtgga
     5521 aaggctaatg gagctttagt tcagggcgac atgtatgttc caagtgccga tggcgttaaa
     5581 ttatatctaa atgccgatcc ggatcttcag gtggtattgg accgcataca gggagccgga
     5641 ggacagatag ttatggctaa gacagccatt ggcgatggtt taggatacat ggcttttttc
     5701 gttgatacgg aagggaatgt agtaggattg cattcgaacg cataatcgta attttgatca
     5761 tggatattac cattaataca ccggcattat tatttcctgc catcagtttg gttatgttgg
     5821 catatacgaa tcgattcatg gcattgagta gtgttgttcg gaaattacat gatgaatatg
     5881 aagccaatag atcgaataaa aacttaccat tacaaattcg caatctgcaa ttccgtatca
     5941 aactagtcag acgtatgcaa ttttttggcg tgacaagttt tattgtggcg ctcataagca
     6001 tgtatctaat ttatgtcgat aacctgcaaa tggcacacat aacatttgca actgccatgt
     6061 tattgtttgc ctgcagcctt gtgatttctt taattgaaat catgaaaagc accaatgcac
     6121 tcgagttgga attgtcggat atggaggatg aagaagggaa atcttttgtc gattacatca
     6181 agaagcatat ttgatctaat aaaaaagccc ggctaaccgg gcttttttat tgcaggtatg
     6241 aaaatgcttt ctaggtttag ggatagcagt gcatttgttg gaataattac cttttaatta
     6301 aatcaacaat gtgaaggtaa tgggggtgca ctatccgaca aacccacttt tagcgagcca
     6361 gccaattttt aatgcgaaat agccgaataa ttgtgtaaac cgaaattatt gcaggttcaa
     6421 agtaaatacc gggccttctc cttttctgaa ttccagtata gaaggtgttg gtgttaacat
     6481 gaaaatgaaa tcctgcccca atgtacaatc tgcagctccg ggggtagtag taccataagt
     6541 aaagattgtt ccagatgggt cgatagattc aaccaccata ttcgcactac atagtacacc
     6601 accgtcgatg gcttcagaaa aagtcaatcc ggttggtgac acggtagttg taaatgtcaa
     6661 aggatgtcca ttagatgcca gtccggtata tacataagtt ccatcaggaa tgctttgtgc
     6721 attggcggtt ccgcacaata aggctatggc aatgaggagt gtgcagagat ttttcataag
     6781 cgctttttgg tgtttggtat ctaaagataa ggcaatttca attcagaggc aacaaccgga
     6841 attgttaatg ctgtaggcaa ggcaattgtg gaaaaccttt taaaattggg gaaaaccttg
     6901 tggcagtagg ctttttcggg atgtagcggc tatatttgca gactatgaaa gcggtgaagg
     6961 aaactccatt aatgggacag tataacagga taaaggccaa atatccggat gctattttac
     7021 tgtttcgtgt tggagatttc tatgagacct tttcacaaga tgccgtagta accagtaaag
     7081 ttttgggcat agtactcacc aagcgggcaa acggatctgc aagtcatatc gaattagcgg
     7141 gattccccta tcatgcgctg gatgcctatt taccgaaact ggtacgggca ggatatcgcg
     7201 ttgccatatg cgaacagctt gaagatccga aactggtgaa aaccatagta aaacgcggcg
     7261 ttacggaatt gatcacgccg ggagttgcca ctaacgataa aatgcttgat accagaagta
     7321 ataactacct ggcaagttat tatggcgaca agcaaatggc gggaatgagt tttctggata
     7381 tcagcacggg ggaatttttt gttgcgcaag gaggtgctga ttattttgaa aagctactac
     7441 atagcttgca gcctgctgaa attattttac ctaagcaaca tcaaaaagca tttaaagaat
     7501 tatatggcaa taagttttat atctatgcca ttgatgattg ggtatatacc gaagattaca
     7561 ccaaagaaaa actgcttaac catttcggca caattaatct gaagggattc ggcattgacg
     7621 atatgccttt tggaattatg gcagccggag catccatcca ttacctcagt gatactgaac
     7681 ataataatat cggacacatc acaggtatct caagactatc ggaggagcag tcggtgtggt
     7741 tagatcggtt taccatccgc aatctggagt tgatacacgc gagttcagaa gatggatcaa
     7801 gtttactaaa ggtgcttgat cactgcatta cgccgatggg cacgaggttg ctgaaaaagt
     7861 atattctcat gcctttgaaa gataagcatg gcatcgatgc acgtttggac atggttgatc
     7921 atttactgca cgacaatgaa cttgccggag ttatcaccaa tgccttaaaa caaattggtg
     7981 acctggaacg tgtggtgtct aaggtttctc ttcaaaaaat taatcctcgt gagattctgc
     8041 agttgaatcg cgccttgcat gcatgtgctc cgttgcgaca ggcgctcatg caaagcaatc
     8101 aacccaccct gcaaaagctt ggcgatcaga tacatttgtg tgaattaatc agagataaaa
     8161 tcgatcgtga aattgctgaa gatgctccgg ctcaagtaca caaaggcgga ctgataaagc
     8221 aaggtgttca tgccgaatta gatgagttga gggccattgg aagttcgggg aaagattttt
     8281 tacttcgttt acaacaagat gaaattatca gaaccggtat tccaagtttg aagatcggtt
     8341 ttaataatgt tttcggatat tacctcgaag taaccaataa atataaagat gtagttccat
     8401 cagactggat acgcaagcaa acactgacga atggtgaacg gtttatcact ccattactaa
     8461 aggagtatga agaaaaaatt ctcggcgcag aagaaaaaat tctggcattg gaacagcaga
     8521 tattcgatac tctgttatcc gaattgcagg attacgttcg acctattcaa ataaatgcaa
     8581 atgttgctgc gcgattggat gtattattga atttcgcaac tatcgccaat gaatatcgct
     8641 atcagaaacc tgagatcaca gaagattttg gtatagatat agaacttggt cgccacccgg
     8701 taattgaaca tcatctgcca attggcgaag gttatgtgcc gaattcaatt tcattggaca
     8761 cagaggaaca acaaattgta atcattaccg gtccgaacat ggcgggtaaa tctgcgctac
     8821 tgcgtcaaac cgcacttatt gtattaatgg cacaaatggg atgctatgtg cctgctcaaa
     8881 gagctagaat cggattgata gataagatat ttacaagagt tggtgcaagt gataatattt
     8941 cttccggcga aagcactttc atggtagaaa tgaatgaaac tgccagcatt atgaataatt
     9001 tatcggcccg tagtttgatc ttacttgatg agataggcag agggaccagt acttatgatg
     9061 gcatatctat tgcctggagc ctatgcgaat ttttacatca acatccacat gcgcgtgcga
     9121 aaacattatt tgcaacgcat taccatgagc taattgaatt ggagcagaaa ttcgatcgca
     9181 ttcgaaacta tcatgtaagc acgagagaag cgggtaacaa agtcattttc cttcgcacat
     9241 tacaacaagg aggcagcaaa catagttttg gcattcatgt tgccaggatg gcgggaatgc
     9301 caccacagat cgtaagcaga gcaaatgaaa tattgaagca actggaagac aagcatattg
     9361 gcgtcgatct gaaaaacaaa ttaaaaaaaa tgcaatcaca acaatttcaa ctgaatatgt
     9421 ttagtgttga cgatccgcag atggcaaaga ttcgggcatt attagaagaa acagacataa
     9481 acaccctctc acctattgag gccttgatga aattaaagga attacgcgat ttgaccggag
     9541 agtaattatc cgcaatcaac tactctacag catcagaaat caaatcccat tcctgctgta
     9601 aattcataaa tggtgttaac cactccttct gcgggcgcac tgtcatcaag caggttataa
     9661 ttgagtttca gatataattt tttggtaata taagatttca catcaagcga tgtcgagatt
     9721 cgcctatcag tataatcatc cagtttgggc tgataatagg ttgtcgacac cagtgaaaaa
     9781 tggtcactcg gagaataggt aaatgacaca taactactca atctattatt ttgttgagca
     9841 tcaatttctc ctgtgttttg ttcgtactcg tacatataga gtgccgcaac atagcacctg
     9901 aaggtatccg ttccatacaa tttaaatcga ggtccggttc cggcaagcaa ccggtagtct
     9961 atagccagcg gttcattata ttgaccttgc gcaaatgcct cccatacaaa tctatcacga
    10021 attttatagt tgtatctaaa atgctggaat ccgctattta caaaatgttg ttcattgtta
    10081 acctgcactg ttgcgatgtc ggtaagcagt aaccaaaggc tcttcttggt tttgtactgt
    10141 aggtgaagat ctgtttgcaa cgacaacacc atgtcattgt ttttattggc ataaagcgac
    10201 aattcgccgc tgcccgacca accaacagta tctgttttga tgcgcttatc ttcaatgttt
    10261 atcacttgag caaaattgtg ttgcacaagc atcatcagta atatcaggcc aagggttcgc
    10321 atggttaatt tttacaaaat taatctaatc atttagtaat gataacaggt aatgcaagat
    10381 tgtttatcgt tttgtttaaa gtgctgatcc tattcttttt aatatcccgg taaaactcta
    10441 attgcatatc aatttgtttt ttcaggtcat aatacaccat tcgttgattg ttgccgggcc
    10501 tgaaattacc ggtagaaaca gcacctatca caccgctgag tttatcattt aatttaattc
    10561 cataattcaa catatcctga ctgctcttat ttctggtttc atataaggta tttattatgg
    10621 catcaaggtc tttgttgatg ttagctatac tatccttcaa agattggggg gccagggaat
    10681 ccagtttagt caaataatca cgcatttgtt tttgcatttc ctgcatatcc aggattgcct
    10741 cttgcgtaga attaaatgtt tccagcattt ccatcagaaa tacatgttgc aatcgatagt
    10801 catcaagtgt tgcagtggaa ttgggatcag gttcaataaa aaaagactgt gtagttgtag
    10861 tgttgccata attgatttca gcagtataat ttccgggagc ggcaagtgga ccgtcaggga
    10921 cgccattcca caaaaacatg ccatctattg atttaccacc cggataatgc atatcccagg
    10981 aaattctgtt gaagcccggc ttaatattta tcgtattgga tttctctttc gcatcggttg
    11041 ccagtgtcgt aatggattta ccttcatggt ctttaatttc tattgaacat ttgcttaatg
    11101 agtctgctcc ggattttgct tttgcagcag gaataaacat gtcgaaaact actcccggtt
    11161 caggattttc gcctgcattt accggtttct ctgttttata catcgacata cgatatacat
    11221 ttcgaaccgg gtaagagata aatgcattat ttactgcttg catttgttgt aacatggtaa
    11281 tattatccaa cacccagaat ccacgaccct gcgtagcaat aattaggtca ttatctttaa
    11341 tagtcatgtc agtaatcggc actatcggca agtttaattg gaaaggatac caattatttc
    11401 ccccattata agaaatatac aacccgcgtt ccgtgccaca atatagaagc ccttcgcgat
    11461 ttcgatcagc acgaattact ctggtaaaat gagtaggtga aattccattg ataatttttt
    11521 tccaggtaac accataatct tcagtgacat ataacaaagg ttgttcatca tccagtttat
    11581 atcttgtggc cgcgaaatac attttgcctt tttgaatcgg atccgtttca atgcaattaa
    11641 tcattgtata cggaggcaaa tctttaggag ttatattttg ccagtcgctg ccattattct
    11701 ttgtaacata taccaatcca tcatcactgc cggtataaat aacacctttt tcaagtccgg
    11761 attctgacgc cgcaaaaatg gtgcaataca cttccacacc tgtattatcc tttgtgataa
    11821 tgccgccgct tggttgctgc atggattttg tgttggttgt aaggtcgggc gacatagcgt
    11881 gccatgaagt tccggcatca tctgtgtaaa acaacacatt accggctgca tacaatcggt
    11941 taggatcgtg tggtgaaaaa aacaatggga aattccattg gaagcgatag gcttgatctc
    12001 ccgccccctg accaattggg tcatcaggcc aaacagtaat atttctggta tcgcgtgtat
    12061 cgtgattcaa acagccgata tatcctgaat aatttccacc ataaacgata tcaggattta
    12121 gtggatcagc tacaatataa ccactttcaa atcctgcagt aggctgccaa tcattattac
    12181 caatttgccc ataggttcca cgatgcctta tgcgcaatga tgaattatct tgttgggcac
    12241 caagaattcg ataaggaaca gcattatctg tagaaacccg ataaatctga gcggttggtt
    12301 gattataata actactccat tcatttcctc cgttgaatga aacttgtgcg ccaccatcat
    12361 cagcaactat catgcgttga gggtcacgat catcaatcca taaaccatga tgatcgccgt
    12421 ggggagttgg aattgattta aaatcacgag caccatttgt acttttccag aattccacat
    12481 tcagtacata aactgtttcg gattgtttag gatctacata aatcttagaa aaataccatg
    12541 cacgttgacg gaggttgcta ttgtcgctgg ttttagtcca tgtttttccg gcatcctcgc
    12601 ttctgaatac acctccggtt ttggactcga tgatggcata ataaatatca ggatctgaag
    12661 gtgctatcgc gagtccgaca attcccaaag tatccttcgg taaaccgctt gcatgtgaaa
    12721 tattttgcca ggtagcgccg gcatctgtag acttataaat tcctgatccg gggccaccac
    12781 tttcgagtga atagggtgtg cgaatcacct gccagaatcc tgcgatcaag atgttaggat
    12841 ctcccggatc catcactaaa tcgcaggcgc ctgttttatc gtcgacatag catactttct
    12901 gccaatgtaa accaccatca atggttttga atacacctct atcggtattg ggaccgaaaa
    12961 gatgtccctg tgctgcaaca tacacgatat ccgggttttt aggatggatt acaatacgca
    13021 tgatatgcct tgtttccggt aacccgagat aagtccatgt tctgccgctg ttcatgcttt
    13081 tccaaatgcc ggttccttcg ctcacattac cgcgcatgct attttctcct aaaccgacat
    13141 atatcactgt ggggtcggat ttacttacgg atattgctcc taccgagcct ccgaagtagc
    13201 catcagaaat gtttttccag ttgttaccac catcttgagt tttccaaact cccccacctg
    13261 tagctccgaa atagaacact tgcggatcgt ccggatcccc gcaaacagcg gtagaccttc
    13321 cacctcgcca agggcctaca tttcggaagt taaggtgctg agttatcgca gtatcgaaag
    13381 gtagcgacag attgctattc tgtttatttt tctgcccgaa ggatgaaatt gttgacagca
    13441 aaagcaagta caataaggat ttacgcataa gacggtataa gctgctaatt acgtcattac
    13501 ccgttaaaaa tcaaagcttt atgcaatgtt tcaagactat ttccattcaa caaaaaatcg
    13561 tcatatcttt gcgccggaga gatggcagag cggtcgattg cggcggtctt gaaaaccgtt
    13621 gaggtgcgag cctccggggg ttcgaatccc tctctctccg ccaaacaaat tatatgcagc
    13681 ctaatgtagg ctgcattttt tatttcaaaa gccagtgaca agtttacttg gcgctggctt
    13741 ttgaaataaa aaatgcaggt gagcgcagcg aatggctgca tgaaattggt ttgaagcccc
    13801 ccatcttagg gatcatggta ataatccctc agttcaataa cttcaacatc aacttaactg
    13861 caaactcatt cctatatcaa agtttacttg gcgctggctt ttgaaataaa aaatgcaggt
    13921 gagcgcagcg aatggctgca tgaaattggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn agcgcagcga atggctgcat gaaattggtt tgaagccccc catcttaggg
    14101 atcatcttaa taatccctca gttgaataac ttcaacatca acttaactac aaactcatcc
    14161 ctatatctaa gtttacttgg cgcaggcttt tgaaataaaa aatgcaggtg agcgcagcga
    14221 atggctgcat gaaattggtt tgaagccccc catcttaggg atcatcttaa taatccctca
    14281 gttgaataac ttcaacatca acttaactac aaactcatcc ctatatctaa gtttacttgg
    14341 cgcaggcttt tgaaataaaa aatgcaggtg agcacagcga acggctgcta atcatttgat
    14401 tgaagccccc atcttaggga tcatggtaat aatccctcac tcctttcaat catgcttaaa
    14461 agtaaatttg caacactcat tcaaaaaaga atgtcactct gagaaataca tccaagtact
    14521 ttatatcgtg aatcagtaat agtgttgcaa gcagttgcat cttctttcat cacacaatat
    14581 ggaaaggacc cgctgctatg gatgaaccaa atgtcatcgc catcattgta aacaaatccg
    14641 gtatgattat cgagtccaat gatatataaa ttctgtttct gtttacgaat attgtcaaac
    14701 atcaaatcac tttctacaag attatatcgc ttgatatgtt tttcatctac caacgctttt
    14761 atcatttcct ctgatgcaca ttgggcaagt ttcaccctgt tgatatcgaa gtgcatgtca
    14821 cgcatcatag tggtaacaaa ataaccgcaa gctatgcttc ccgagccggg cacttctgaa
    14881 atcccattga aattccagcg ggtgcctaac caatatggat aacgatcgtt aaatgcttga
    14941 attagtgtga cgccggattc cggttttgtg gtgctttcaa ccaatagttt ataatcttcg
    15001 gcgctatgag ctgctctata ccatggtgca taaaatgggt acttggcaat gaaagcggcg
    15061 gccagtatca ataagattac aactaggatg aggaatatgc gaagaaatgc tttcatgagt
    15121 gtgtattcgt gaagataacg aatatttaaa tggtgatatt atagattctc ctacctttgg
    15181 cataaatcaa gcgcaatggc agacatcaaa accattaata acagcagtga tatcatgcac
    15241 tgttttgaag tgatccatac ccttagacca cacctaaatg aagaacagac aattgcttgt
    15301 tttgagcaga tgcgctcaga aggttatcac gccgtgtatg ttgaagataa cggcaaagca
    15361 atggcatact gcggttatcg gtttatcact cattacttta gtggatcagc tatgtatatc
    15421 gacgatctgg taacagttgc ggatgcaaga aataaaggat atgctagttt gttgcttgat
    15481 catgtgattg agataggcaa taaaaaaggg ttggaaacga tcagattaga ttccggacat
    15541 cacagacacg atgcacatcg attatatctg aataaaggat ttcatattca ttcccataat
    15601 ttcagtatga cgctaaaaaa ataaactatt cgttggtaag tagcagatac tgatatggcg
    15661 tcagtgcaag ttcagtgcca agtgagaccg aatttccgtt aatagcatca gtccattcag
    15721 tgtcttttaa cgcggtaggc aatgtatagg taacatctgc ggaacgcgca ttcaccaaga
    15781 caacaaattg atcgcttgcc aaagtttttt taatgcaata aacatctgca ttgctgaatg
    15841 tggtaagtgt tccggtacgc gcagtctgat gtccactata agcatttaat atctgtttat
    15901 attctaactg catttctgga ttcaaagtcc aatttatagg agcgttggaa aagaatggca
    15961 catttgcggt gttacccgtc tcttgtccgg tatagatcag cggtactcca tttaaaaata
    16021 tggtcatcac agacgccgct acggcgccat caatgccatt gaaaagcacc atcggagtgg
    16081 cattccaagc cgactcatcg tgattcgtag taaagcgcag ttttttgtcc cctgcaggca
    16141 caccagcata ttctgcatta ttagtggcat acaaatcaga tacggcagag cccgaataaa
    16201 cattttgcat ggtgccatag aaatcccatg cataggtcat atcaaagcct gctgtgtaat
    16261 gatcatcgcg gtcgccttca gctagcatga tcaatgttcg atcaggaata ttgcgcaaag
    16321 aatctaaagc ctgctgccag aaatcaaacg gtacgccatc ggcataatca catctgaacc
    16381 catcaacatt accaaccaat acccagaatt tcattgcatc aatcatttga gctcgcatat
    16441 cctggttatc aaaattcaaa tctgccacat cggtccacgt agttccgggt ggatgaataa
    16501 tattaccatc agcatcttgt gtataccaat cgggatgatc tgtgatccag tcattgtccc
    16561 atgaggtatg attagcaacc caatcaagta ctactgcaat atttctggca tgagctgcat
    16621 ctgtcaactg ttgaagatcc tgtaatgatc catattcatc actaacctga taataatcgc
    16681 gaatacaata cggtgaatgt actgccatca attcaccttc atcataaatc ggcatcaacc
    16741 atatggtatt caccgatata tcctttaagc gatcgagatc tgcgattata gactgtatat
    16801 ttccggcagc actatatgcg cgcaaattga tctcatacat gacgatgttt cctgtttcag
    16861 gaacactatc aaatggtgat ccatattgat catattgatc atcaagtgtc ggaaaaatat
    16921 tgccatcctg attacaagat gcaaatattg cgcttagccc tataatgatg ataatatgtc
    16981 tgaatgacaa cctatattgc atggcgaaag tatttcgctt caattattgt ttatagatag
    17041 acattaactg tcgggtaact ttcgatttct cgaattgttg tgcgtgcaat ttccctttgt
    17101 taatcatatt ttgacgcaat tgttcatctc ctactacacg ttgaatttga gcagcaatat
    17161 cttcagggtt ctcgggatta aaatacaaag ctgcatcgcc acctacttca ggaaggctgg
    17221 aaacagagga cgtaataaca gggcagccac tcaccatgga ttcaaggata gggatgccga
    17281 atccttcata caaactcgga taaataaaac acagcgcatt agcgtacaaa gcaggcaaat
    17341 gatctgttgg tacattttca agaaaaataa atgatgaagt caagtgattc gctgacacga
    17401 aggctcgcac ttcatcagca tatcgcttgc cattaccaac tatcacacag ggcaaacgat
    17461 cagcagaagg taattttgta tatgcattac aaactgcgag tagatttttt ctttcagtaa
    17521 cggatcctac ataaagcatg tattgttttg gcaatcgata acttgaaata tcagctgccg
    17581 gcggtgcata aaaaatatca tcacagcttt gatacacaac agatatacct gcaggatctt
    17641 ttccgtacat cctcaccaaa tcattgcgtg tactttcact tgctgctata acaacatcag
    17701 cattatttac tgcaaatttt gttttcagct cataaacggt tctatctaca ccgggatagt
    17761 gatgagggaa cagatcgaaa ataagatcgt gaatggtaac tacactttta acatcagctt
    17821 tattgatgct gaatggtaat tcattactca agccatgaaa cacatcaatt ttatgtttca
    17881 ggatatcccg tctgataccg aagctcctcc aaacagatcc tcggccaatg acagttttga
    17941 tattcccttc agcattaaat gaaagttgat ctttagcatt cggggtaaac aaaaagtatt
    18001 cattctccgg aaactgagag gcaaggttct tcacgactgc acggctgtag ttgcccaagc
    18061 cggtggcatt ctgaaaaatc cttttagcat caaatccaat tctcatcagt agtaacgggt
    18121 atccacaacg aaggtaatga agaatgtgac aaacataaaa aaaacccctg ttgatgacag
    18181 gggttctaga tatggtcaat aagttatcag ttgatcacaa atttaccgga ttggctttca
    18241 cttattccgg tgaggcgata taaatacata cctgcaggca gttcatcgcg attgatggtg
    18301 atagtattgc cggtagtatt aagtgtgcgc acaacatttc cattgatatc ggtgattaca
    18361 aggtcaaaga cctggtgtcc ggcattgctg aaatgcagtt ctgccgctgt gctgaaagga
    18421 ttcggttgta tgctaatctc aagcgggttg ctggcataat actgtatacc tgtttcatcc
    18481 actgtcacac agctgttatc aagggctgtc actacctctt cgaaatcttc attcactgct
    18541 gctgtaaagt gtttgataca tatttcagta ggatatgtag cagtatagaa aattcggaag
    18601 gttgggatag gctcaaagtg aatttgataa ggcacttcat tatgaccaag tgcaatcact
    18661 tcattgtcgg tataagtgag catataattt tcaccaaata cttcaggaca aagattagcc
    18721 actgagtcaa tagtaatacc ttccattgta aattgtgcac ctaaaactct ggcacttgga
    18781 ttcaagctat aaatatcaag gtatccgaat ggtgcattta ccgttcctat gctgtaagtt
    18841 acggtatcag atgtgttcat cattgcaacc gggagttcgc agaggtctgt atcatgtgta
    18901 taaccatacg tttgattagc gcagcccata acgagtgcag catccactac atcgatatcg
    18961 ctatcattat ttgcatcgtt acacatatca gcgatgatgg tatttgagag aatgttattt
    19021 acgtacaagt caacatcatc atacaaacgg gtgctatcct cattcaaatc gccaatgtga
    19081 tgccaaccgt tacagttgcc ttcacagtcg agttgtgctg ttccccaaat ttctccgaga
    19141 caatcgatgt acagcggaca atcatctatc atttctgcat tgataacacc tgcgtctcgc
    19201 cagatatgaa tacaacgtga acgccagttg ttggctaggt tggtttcaac acgtccttcg
    19261 atatcagggt gattttccca attcacacgt gctacgaaag tataagtgcc atcaggaaca
    19321 tcggtgatat caatccactg tccgcctgta cctaatccgt aagcatcaaa gcaacctgca
    19381 gaaataccca tatcagcacc attatatcca ccgataccgc aaagatccat cacagcaaaa
    19441 ccgtttttat gtccaaccgg aatttcagtt cctaaagaat catataacag atattcacca
    19501 tagtctacat aatgggtatg tccatgacaa ggatcaaata ctaaatatgg attttctgca
    19561 gctgttccga tatgataatc ttcgtcgccc agattccaga tttttattcc aaaacgaata
    19621 attgttctca taccgggacc ggtaagtgaa ccttcatcaa tataacagga gttcgtccag
    19681 tcatcggcat ttattgtcga aagctgaaaa tctgttcccc agcctgtgct gatgtcacca
    19741 tcaaaatatg tttcatccaa taccaggtcc ggtccggaag ggcaatcagg atttccggga
    19801 tagatacaag agccatcgtc aaccattgct ataggtgaat agttacaggc aaaagcatca
    19861 atacaacctg taggcaatcc tgtataggca atcgaccaat taatggactc gccggcgcag
    19921 gccgtttcag catcaccaat acgaatgtat accaaatctc ctgcctcaag caaggtagtt
    19981 atttgcgatt gagatccgca ataatcatca ttgtaggata aagtgccttc tgcaagctct
    20041 gttggaatta gtccaacaca atgatcatag acatagagcc tggtgtcgca ggtgtttccc
    20101 atatcgcagg tagagatcac ataagttccg gtagtaggaa cagtaaagct ataccagtaa
    20161 tcagggttag gtgccgtgta ggttccttcc gttattgaaa cggcggtatt acaatcctct
    20221 ccaaccatca gaggcgcagc atatgaaaca gttgctgcag caattgatgc tgaaaggaac
    20281 aaggcagaaa taatgcgagg agtacataga ttaagcatac gggtcgattt tctccgataa
    20341 atatacagaa attccggctt ttgaagcgga ctcagggtaa ggattgagcc aaaaaaggtc
    20401 ttagtttcat tttaaagccg ttaattcctt agccaccgct gcaacagtgg aaggggcatt
    20461 accaatgaaa atcttctctc cgatgataaa aacaggcctc cttagaaacg tgtattcctc
    20521 aagaatatat ttcttgtaat ctttctcgga aagcttcttt tgattaagcc ccaatgcccg
    20581 atacttcagg gcaaccctgc tgaacaactg ctcataactg ccggctaagt ccttcattct
    20641 gtcaagttgc ccgggcgtaa tttgttctgt tttgatgtct tgcagtctga aaggatattt
    20701 ctgtactttc agctccttca ttatacgttg acaagtgctg catgatgcca gataaaataa
    20761 ttcaagcgac ataaatagaa aacaatagca ttattaaatg gtttaaacgg ttccatgttg
    20821 ttcatcctct ccatcccgag aacgaagcac acggtttacg tcaacgctgt tatttatttc
    20881 aaatccaacc agtaatacca acgcattaag atttatccat atcattaaaa cgatcaatgt
    20941 tccgatagat ccatacacct cgttgtatcc gatcaggtta ttggcagcat atccaaatat
    21001 tacagatgct gcgatggaaa caattgttgc aaatgtgcct ccgggtgtaa taaagcgcca
    21061 acgatgtttt acagccgggc cataataata tatcaatgat attccaaaaa agaagaggat
    21121 aaggatgata gcatattgaa aaatgttgag aaagaacaca cctgtattat cttcccagtt
    21181 gatccaatcg ccaaccacac ccactacctt atctcccata acgatagtta taattgaaac
    21241 gataaacaat aaaaataaat aaaaggttaa tcggaaggca gctattctgt tgcgccagaa
    21301 atttctgcgt tgataaattg gatgcgattt attaaatgtg agcatcaaca tgcgcacgcc
    21361 atttgtagaa aagtaaaatg ctaagataaa acccacagat aacaatccgc cacgctttat
    21421 acttgttata ccggtaatgg tagactccag aaaagcatac ccactttcag gcaatacatc
    21481 tctaaaaaaa tcatgaatat ttgcatctaa acctttaata ggaatgtaag gtattaaagt
    21541 aaacacaaag atgatagatg gaaatattgc taacaggaag ttgaatgaaa tcgcagatgc
    21601 acgaacaggt aattgatcac gtcgaatctc ctgccagaaa aagctaataa catcataaat
    21661 gggaataccc gcaaaacctg gaaagctggt ccgcttggta aaattgacca cacgtgcgta
    21721 tttattagct acgagtcgac gaatgtttga gggaatgatc ttcatacgct gtggttccga
    21781 cacaaaggta tgattttata tggatcgtag cttctgtgcc agtaggatga ccgccggatc
    21841 atcggcatca tatcgctgca aatactccaa agatgacttt gctttcgact tttgccgcga
    21901 ctgtatatat aattccgtga gtttgatgcg cgaaagggta taatccgggt tcaaggcaat
    21961 agcttttaat aagcactgct ccgcttccgg catctgccct tttgttaata atataacacc
    22021 aagattattc caggctttct cgtactccgg attctcccct actatcttcc gatatacagc
    22081 ttcacctgat ttattgtcac cactaaatac catagccgtt gcttgtttga acagataatc
    22141 aaggtttaat ggctcgcgtt ctactgcaat attgaaatat tttagtgcag cagcaaagtc
    22201 acttttcgca gtataagcct ctcccattct gtaagcagtc catgcgttta atttgtcttt
    22261 cttaattttc tctcccgatt gaataatttg atccggtgca tctttcagat aataataatg
    22321 cacttttacc gagcggactt catcattttc ttttcctgct ttttgaatgt attgccaggc
    22381 actatcaagc aaactctttt ccttattaaa cgactcataa taatgcaggt aaccccgcgc
    22441 catggttagc ggatcaggat tgctgtcagt taaacacacc aaacctttaa atgtcccgct
    22501 acctttagat gctgtatttt ttggtatgct gatgatgtga tcagaaatgg acacgtgtgg
    22561 gatatcaatg gagccgctct tccggatatg gcaactactg caatcatctg ccacattatt
    22621 acgcgatgct attgatgctt tgcattgtgt atttccttcg ccatgacatt gcttacatgg
    22681 agcattgaat tgttcgcgag gtgtatcttt aaccgtaata tgcggattat gacatgtgat
    22741 gcaactcaat tgatgtgtag aagtatagca tttcgattgc agtaaacgat ctgcctgcga
    22801 cgccatcaaa aatttattat tactgccatc aaatcgcgga agaaaaatat tccagtgctc
    22861 attgatatgg tctccgggtt tgaaatcgaa aaatgatgca ccttcattca agacattaac
    22921 gccttgtaaa tgacatcgca tgcataattg attttgtaac tccacagata attttcttgg
    22981 attcacaatg ctgtagtcaa tatttttcga tgtatcaacc aattcaccat tacttactct
    23041 ggcgacatgc agacttcccg gaccatgaca acgttcgcaa tcgattcccg aaggcacctt
    23101 tgcatattta tttacagatc cttcaactag ttcaggcaat ccattgtgac aggtcataca
    23161 ttcagcttct atcactcttg aaaaccttga gttgaatcca ccgctcatgc ccggagcgag
    23221 atcccattgc tgtttttgtg tgtagaaagt gattggcgcc tgataaacat atccattcac
    23281 ttgcagcata tgcgagttgg tatgctgccc gctgccaatg atgaaggcaa ttggttctat
    23341 acgcttgtga atggtatcct gaccatcaag tctgaattcc attatttgca aggtatcatt
    23401 tcgccagaat ggtttgtaat aaaaattatt cgtgctgtcg tacaccaatg catgttcagc
    23461 atcgaacaaa gcagcacttt tcgatttcgt cgccttatcg aaactcatac ccataccggt
    23521 atgaataaaa gttgcatgtt tatcggcgtg gcatagttta catgtttcca tgccaacata
    23581 cgagactgta tcgtttgcat tgagatacac attttcggtt gcttcaccct tgttgttggt
    23641 gcaaaaggta agcatactga caaatgcaca aacgatagct atggcaatgc ctgttcgcat
    23701 gatgatgtaa aaataggtga tgcaatcgtt gcttacaaca aatcagctat ccagaaatcg
    23761 agctgcaata tccggcgtag gaatcataca cgactcccgc ttcccaaacc agcgatagcg
    23821 acgctttgca acaaaacgat acaggggatc tctcaggaac cggggtatta tccatcctgc
    23881 aacaaacagt ttccaaggga aagccagttt acccgccacg cgaagcaatg ctgttgtctc
    23941 gtaaaataat ttaccatcct cccacaagat tattgaatca atatttgggg caataggatg
    24001 ttcctcgaaa aagcgctttg cgaattccga ttgcagcgaa gcgaatttga aatacgcatg
    24061 cttatcactt cggagaataa cattcaccga ggcgttgcag aaattacata caccgtcaaa
    24121 aaaaatgatg ggttgatttg tcatttacca acagtagcct gccattcgag catgccacca
    24181 agcaggttgc gaacattagt aaaacccgat tgcaccatga gtgcttttgc agatgcagag
    24241 cgcataccgg agcggcaatg aacaatgatc tcgcgatctt tatcatcatc gaaatcatac
    24301 atcttcatag gaagcgatgt gagcggaatg tttatcgccc caatattaaa ctctgcatat
    24361 tcatgcggct cgcgcacatc aatgatcagc aggtcttcac cggcatccat tctttttttg
    24421 agttcttcta cagaaatatc catgtcctga aaattgcctg aaattattgt ttaacaaatg
    24481 tggttgatgc aagcattgcc ttttgcgcat ctaaaatttt gagtatatat attcctgccg
    24541 ataaatcagc aatatttatt gttccggaag tgatgtcgct caccaaaact ctgcgtccat
    24601 ctacatcgaa aatttctatt gattgcattt cttcagtggc agtgttgata ttcaatatat
    24661 cagttgccgg attcggatat accattaatg atgatgtacc gggcgattca ttttctaccg
    24721 gcgcatacat cgaataattg ccacctaatt gtggtcgcaa cataatagca ccgctgagtg
    24781 ccgattgctg ccaggtgccg ccaacattat agaatacgtt ttctttagca tcgctgttta
    24841 catcaaatcc aatatccaat gatgcttcct gcgattgcaa ccatcctaca taaaaatatc
    24901 catctacaac aaccggagcc ggattaccaa gaaaatctac gagcttatac acgtaaaatc
    24961 cgttgatgct atcgatgtat tttggtgtaa ggaaatcgtt ttggtaaata atattttcgt
    25021 cggcagttgc tcctgtagta tcaatattat cccacaatag gaggctaaaa aatttattgc
    25081 tattgctgcc atcaagatat gcccagtgaa tataaatttc cttgatcgta tccggctcgt
    25141 ttgcatagaa tcgcatggca agttttgagc ctaatccaat taattggtat gcgagctcag
    25201 cagaaccgtc atcgtaggcg aagtaattgt aaaacgactg atcatgcaac aaggtatcat
    25261 tgcggtgatt gatatcgcct gaaggttcca gctgatattt gaatgtgatt gacaaactat
    25321 catcattata accaggcgta gcctcaggaa tttcaaaatt cggatagcta tcaaaagcta
    25381 tcgtaaaggg atccaggtta accgatacag gagctgaaag cggcattacc tctgtgcctg
    25441 aatatttttc tacaacactg tatgcatgag aagtatttac aatcgtgttg aaattattat
    25501 acatgaacac gccatgtgtt gcagaaatct cttctgcctg atgatcttta aattgattcc
    25561 atggcatctg gcggtatctg cgcaaataag gctccggttg gtatatgata gccacatcgc
    25621 gaaacaaggt atcattatag gtgcgatcat catcgagata tacataatcc aaatgccaat
    25681 gatcattatt gccatttata cctgcccaat tgcggaagcg gaattgaaat ccatcgtaat
    25741 gatacgcatc agcaacagga atcatcacca atttaaatga ttgcggatca tcgcctaaag
    25801 tatttacggt aaaccacaca ttcgaccatg tgccgtcttc agctttaaaa tcgagtaaaa
    25861 gagaatcgtt gagatttgga tattcgccaa atccgccggg ttgataataa aaactcagat
    25921 aaactgaatc cgtaatcgca ccaccaacaa aaattggttt tgatgtgagc atatctgccg
    25981 gtccgagcgt tgaggtttcg ttatacggct gaccatattt atttaatcca tcgagtgtgg
    26041 caacaccata ggttggtggt aaggatggat atgttctatt tacgaatgca ttgctatcca
    26101 cccaaaaaat tgatgaagga taaatggacg atgaagtttg tataatacta tctccacact
    26161 ccgtaaaggc accatcaaca aatatctgag ctgcgctaaa atcatccata aatggcatgc
    26221 agagggtatc cctgttttct acgatactgc gacttcctgc aacaacttcc ctttgcatca
    26281 aagcggtatt atttttcaag ggaaggagaa tctcctgtgc ctgcaattgt atcggaagat
    26341 agcaagagag tgcggcg
//
LOCUS       DAOQOU010000028        26227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_859_length_26227_cov_5.573705, whole genome shotgun sequence.
ACCESSION   DAOQOU010000028 DAOQOU010000000
VERSION     DAOQOU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26227
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_859_length_26227_cov_5.573705"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLM90_04175"
     CDS             complement(<1..410)
                     /locus_tag="PLM90_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88595.1"
                     /translation="MRLPPVILGFFALGLTLIACRQDDAVYLQAPENKYILCRGSALY
                     LDNGKRWKSNIETTEGIDSMIVQLNKFPDDADSAAYQALGSELVEDLIYIVNHCEQMG
                     NNHEMVHAYLNPLQELIVPLQVSGTETCKAQLPK"
     gene            537..1484
                     /locus_tag="PLM90_04180"
     CDS             537..1484
                     /locus_tag="PLM90_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ88596.1"
                     /translation="MNDNSLLWLGFNAFVLLMLALDLGVFHRKSHVVGIKEALAWSGV
                     WIALALVFNGLIYFGIVGGYEGAELQKQKGMEFLTGYLIEKSLSIDNIFVFLLIFTFF
                     KVPQLYQHKVLFWGIIGALVMRAIFIFAGVALIEKFHWIIYVFGVFLIITGINMVRNK
                     NKQLDPEKSLVIKGFRKIFPVHNSYDGSKFFVTLNGKRHATLLFVVLVFIEFTDLIFA
                     VDSIPAILAITPDPFIVYTSNVFAILGLRSLYFALAGIMHRFAYLVYGLAAVLVFVGL
                     KMVLVDVYKIPVQYSLLVIAILLAASIFYSIYNTRKMKA"
     gene            complement(1495..2700)
                     /locus_tag="PLM90_04185"
     CDS             complement(1495..2700)
                     /locus_tag="PLM90_04185"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ88597.1"
                     /translation="MFGKALNIYLDSYRGINRSVWILAAIQLINRSGSMVLPFMTVYM
                     TANLGYSTVQAGIIMSVYGLGGLLGSYLGGRWTDKFGPFQVQLFSLLFGGGCYLLLPL
                     LTGFYSLALGIFVCATVNDAMRPAASSMIGHFATKETTTRSFSLMRMAINLGVAIGPA
                     VAGLLAETNYFWLFFGDGITCIAAGVVFFFYFRGKRPQIHKEDKQEDQPQKSPYTEPA
                     FLYFLFLCFCYAAAFFQIFSSIPLYYKDVYFQSEKAIGLLIGFNGVIVFLFEMITVSQ
                     IEKRFKPERLIIAGTLMLGVSFAIFNLFTGMWVLITAMTLLSFSEIFAMPFMISHVVK
                     TATPQTRGSHLAAYTIAWSVAFIVSPYCSTQIIDKLGFDALWWTFGIFCAIIAIGFLK
                     VRGIKSDVE"
     gene            complement(2760..3329)
                     /locus_tag="PLM90_04190"
     CDS             complement(2760..3329)
                     /locus_tag="PLM90_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HMZ88598.1"
                     /translation="MRKRKIAVGITGASGAIYAKVLLDKLQLLGAEIEKVGVVMSDNA
                     KDVWRYELDNEQFTNYPFTFYGKNDFMAPFASGSAWFDTLIVCPCSMGTLGRIASGIS
                     NDLLTRTADVMLKERRKLILAVRETPYNLIHIRNMETVTTAGGIICPLSPSFYSKPKS
                     QEEVAATVVDRILQLADFSVPMYRWNDPS"
     gene            3408..4046
                     /locus_tag="PLM90_04195"
     CDS             3408..4046
                     /locus_tag="PLM90_04195"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ88599.1"
                     /translation="MQKRILISAALIMLLGAALMAFVPMDTLKEASGADLMMATTVED
                     SAKTEFKWYTISEALEMQKTSHKKIFMDVYTTWCGPCRMLDANTFSNPEIQKLLSEYY
                     IPVKFNAESGDTIQYQGQTFTNRNYSPLPRKSTHDFAVYIASTEQGLGYPTVVFMDET
                     GTRLQPISGYREAGKGTAQNPGMEPILSFFGTDAYKTATWNDFYAAFQSNIK"
     gene            4050..4640
                     /locus_tag="PLM90_04200"
     CDS             4050..4640
                     /locus_tag="PLM90_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HMZ88600.1"
                     /translation="MFIEIALSLVMFFHEDHQETIAPAAEQAGVDTIGWMSLSDVNKI
                     VEKNIKKGKVKEDKLVIVDFYTDWCGWCKKLDAETYKDPEVAALMNKYFYPVKFDAES
                     KDTVAFANKTYGFKSSGSRGTNEFALEMASRPGGRIGYPTITIINALGEKLTVEAGYK
                     NPEKMKLMLLYYGEGHYKTKDFNTFVTEYNQKQLTD"
     gene            4737..6686
                     /locus_tag="PLM90_04205"
     CDS             4737..6686
                     /locus_tag="PLM90_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HMZ88601.1"
                     /translation="MSSAAKNIHKLSAAGVLISLGIIFGDIGTSPIYVISAIVGDHVI
                     SRELILGGLSCVFWTLVIITTFKYVILALNNDNRGEGGIFALYAIHRRFKVKWAIVPA
                     LVGCAALIADGFITPPISISSAIEGLKLLNPNIDSVVPIVIIAMIILFTVQQFGTNVV
                     GKAFGPVMSIWFLMIGILGSIQVVQHPEVLEALNPYWAIHLLTEYKGGFWLLGSVFLC
                     TTGAEAMYSDLGHCGKQNIRFSWTFVLTMLLLNYAGQSAYMLTMDGQQLNDHSPFYMI
                     MPTWFLPIGIIVATSATMIASQAILTGCFTLVNEAMKLKLWTNFKVVYPSTVKGQIYI
                     PAINWFLFAGCVTVVLLFREAKHMEAAYGLAITFDMIMTTVLLTSLMLLHKRAKLMVF
                     SLAALFLLIEGSFLVSNLNKFTHGGWFTVSLAVGIFFALWIFYEARKLRAKHTEFVEI
                     DKYLPAIQDLMHDESIAKEATHLVYLSMVNDKNHIDSNIIYSIFRKKPKRADVYWFVH
                     VDISNDPYLATYSVDTIVPKKIFFVRLRFGFKVEHKVNVMFRRVVEEMVAAGEVDLLS
                     NYPSLRKHGMYADFKFILLNSRVSIDNKLSPWEQIVIKGYRFFKSLSLPTHEDFGLEL
                     TNVEYEMVPIKVSQEIEIKLERVTK"
     gene            complement(6676..7419)
                     /locus_tag="PLM90_04210"
     CDS             complement(6676..7419)
                     /locus_tag="PLM90_04210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HMZ88602.1"
                     /translation="MRRFAISDIHGCHQTFSALLDTLQLTKEDELYILGDLIDRGPNS
                     IAVIDQVLKMVEEGYKVTTLMGNHEWMFMQALDETIPYCWDWRRYQAWVKNGGVTTMM
                     NLGYKRIDDLKNIDKKYDTWVRSLKPYIELDKVILVHAGFNFKEEDIFKDTHSLYWIR
                     NYYDTIIPEKINNKIIVHGHTSRPFNTLEQELSEMKYPAVNIDCGCVYVGRPGYGHLC
                     ALDLDTMRPIFQKNVDRIEKPVASEESTL"
     gene            complement(7424..9931)
                     /locus_tag="PLM90_04215"
     CDS             complement(7424..9931)
                     /locus_tag="PLM90_04215"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88603.1"
                     /translation="MKSIHLLILACSMLHFSVHAQMESITLDQQWQFRRFGDTQWMKA
                     KVPGCVHTDLLTNNAIPDPFIDTNEAAVQWVENEDWEYRTTFHLHKNQLNSDFIDLNF
                     GGLDTYADVYLNDSLILSSTNMFRAYKVPVKTLLKSGDNLLFIHFYSPVKRANEVGEQ
                     FTVPLPSDERVYIRKAQYQFGWDWGPRLVTSGIWQPITLVFNDKLKIKFVDFYTEQNP
                     DYQFPVHIDLAYEALGRMHYEVEVKELTTGKTCYSWSYRDQLPTVEQINFNFHAPEVW
                     MPYALGSQNIYAFELKITHNKRIYYQQVFKTGFSNIQLHQEADASGKSFYFTANGKKV
                     FINGANFIPAHSFPTEASDERYRDILIEAKRMHINMIRVWGGGIYEQDIFYDLCDSLG
                     IMVWQDFMFACAMYPNDVTEYPEFQQQITRLKNHPCLALWCGNNEIDEGWNNWGWQKQ
                     LKYNDDDSAYVASLNEKLFRLVIPEYLHSNAGAYQSYHPSSPTYGWGRKESLTSGDAH
                     YWGVWWGKQPFAIYNKKVPRFMSEYGFQGIPSYHSWEKCINSDNLYLGSPAFKAHQKH
                     PVGYETIDEYMKRDYGTPSDLQSYIYISQLLQADGMRVAIEAHRRNMPYCMGTMFWQL
                     NDCWPVTSWSVQDFYGNRKAAWYTVESCYRDVIISVLQEGDELQTWLVNNDTTDRKLQ
                     LTYSLLTTSGVTIRNQTLPVTLKANTSMLYSTLGVEWFLSGYAKENCILWLSLSEEDK
                     VIAERMHTFVRPGAVSYKKADIAISIDGDVITLTSPVFARQVYLYSDDTELRLSNNFV
                     DLLPNTPKQISLQDISEGTIPDVSKLKVLCLNNLKLN"
     gene            complement(9928..10686)
                     /locus_tag="PLM90_04220"
     CDS             complement(9928..10686)
                     /locus_tag="PLM90_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HMZ88604.1"
                     /translation="MKTSVTRKYILEICTFNATDVVHAVAAGADRIELCASYVEGGIT
                     PSSAAITAAMQMIDPENIVVMIRPRGGDFMYDDFEFDVMRHDILHCRSLGVKQVIFGI
                     TTPDGKLDVERNSRLIDTASGMQCTLQRAFDLTRDPFEALEDAITCGFTRILTSGQAN
                     TAIEGKEIIKQLITSANNNITLLPGAGVNSSNAREIIDYTGCNEIHTSAKRPFTPKST
                     SYNFNSNIYDTSHLTEVNPEEVRKLSAIIATYNA"
     gene            complement(10687..11682)
                     /locus_tag="PLM90_04225"
     CDS             complement(10687..11682)
                     /locus_tag="PLM90_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HMZ88605.1"
                     /translation="MTSRRSFIKTAALGAGVISTGQSLQSAVVENQHKSVPMNKPLVL
                     STWDFGLKANVDAWAILSAKGRALDAVEAGVKVAENDLHSLSVGLAGLPDREGHTTLD
                     ACIMDEHYNCGSVAFLEKIKNPISVARMVMEKTPHIMLVGSGAQQFALENGFTLDDYK
                     NPEAQKAYEAWLVEKQYKPVINIENHDTIGMIAMDMQGNLSGACTTSGLAYKVRGRVG
                     DSPIIGAGLYVDNEIGAATATGTGEEVIRIAGTHLVVELMRQKYSPYEACKEAVMRMV
                     KIRKEKTKDFQVGFIALNKAGEFGAYCLQPGFNYAVFSNNIANELFKSDSYYPEQ"
     gene            complement(11761..14058)
                     /locus_tag="PLM90_04230"
     CDS             complement(11761..14058)
                     /locus_tag="PLM90_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HMZ88606.1"
                     /translation="MKTTLIFTLLLTLHVNMLKAQQCPIVPKPVEYTANPGYFYFKPT
                     SYILVNDMASFMDAVTFRDMSIKFHSLPLKTVREEIYPSHYIEVSYDSTLMIPDGGYT
                     MLITEEKITIIGKNKGGVFYGLMSLLQMTESKFTNQFQVPCAGISDFPQFQYRGMHLD
                     CSRHFFTVDEVKMYIDYLALYKMNTFHWHLTDDQGWRIEIKQYPKLTSVGAYRTGTMK
                     GHYRDQQYDTINYGGYYTQKEISEVVKYAKNRNITIIPEIEMPGHCAAALAAYPELGC
                     IADTTYSVGQAWGVYKEAFCPKPETFEFLYRVLDEVMALFPSQYIHIGGDEVEKDQWM
                     HCDYCQQLIKEKNLVDEHGLQGYFTKTIAEYVQSKGRNIIGWDEILTNDLPQQAAIMS
                     WRGVNGGIDAARAGHYAVMTPGSHCYFDYYQGLPYQEPLAIGGYIPLQKVYEYHPIPD
                     ALTPEESKYILGAQGNLWTEYIPNFDQVEYMVIPRMLALSEVLWTPDTARNYADFMRR
                     LSTQFELLGRVDCHYSKSAFGIIPEVLPTDDFNGVRIALRTWDNTTGFEYQYEDTSKN
                     SPAVFGGAFSKGIYTAPFIVNESYSLIIPEDYKHPEVKLRLLFNKATGKKITLKTPPA
                     QQYSGSGAFSLVDGINASLYPSWSGNEWLGFSGTNLNCTIDLGAVDTIMAVSAGFLED
                     KTSWIYLPSSMEISVSLDGKKFTRIGIVPVVKDDRAITNGIYKFDPTAARYVRIIANN
                     IGTIPQGNPGAGNKAWLFVDEVHIR"
     gene            complement(14068..15711)
                     /locus_tag="PLM90_04235"
     CDS             complement(14068..15711)
                     /locus_tag="PLM90_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ88607.1"
                     /translation="MTKETQNIAFNRNEDALRLLVSTMKQRLAKVQEGGGKKSIDKLH
                     EKGKLHARERIKLLFDMDTPCIEIGAFTAMGMYTEHGGCPSGGVVVMIGYVSGRQCIV
                     VANDATVKAGAWFPITGKKNLRAQEISIENKLPIIYLVDSAGVYLPMQDEIFPDKENF
                     GRIFRNNAVMSSMGITQIAAIMGSCVAGGAYLPIMSDEAYIVKNTGSIFLAGPYLVKA
                     AIGEDIDAETLGGAITTTEISGICDYAAENDEDCITHIRSTMDKIGAMPKAGFDRIAP
                     SAPSLKEEDIYGIMPEDITKQYDVLDIIKRLVDNSVFDEYKAAYGKTIVCGTARIDGW
                     SVGIVANQRKVVKTKKGEIQIGGVIYNDSADKAARFIMNCNQKKIPLLFLQDVTGFMV
                     GSKSEHAGIIKDGAKLVNAVANSVVPKFTVIIGNSFGAGNYAMCGKAYDPRLIVAWPN
                     AKIAVMGGAQAGKVLLQIQVASLKAKGEEITAEKEKALLEKILNHYNETMDPYYAAAR
                     LWIDGIIDPLDTRKWVSMGIEMANNAPIEKDFSVGVMQV"
     gene            15976..16632
                     /gene="fsa"
                     /locus_tag="PLM90_04240"
     CDS             15976..16632
                     /gene="fsa"
                     /locus_tag="PLM90_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HMZ88608.1"
                     /translation="MKFFIDTADLNEIKEAASLGILDGVTTNPSLMAKIGITGAENIK
                     QHYIDICNIVDGDISAEVISTDFEGMVREAKELVALHPNIVVKIPMIKDGVKALKWCT
                     DNNIRTNCTLVFSAGQAILAAKAGATYLSPFIGRLDDINWDGVQLIEQIVHIYRNYGY
                     ETQVLAASIRNPLHIIRCAEVGADVVTCPLSSIMGLFKHPLTDIGLAQFLADYKKGNG
                     "
     gene            16782..18065
                     /locus_tag="PLM90_04245"
     CDS             16782..18065
                     /locus_tag="PLM90_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ88609.1"
                     /translation="MHRLPSDEVLPFLLMVATLLLAARLMGELFRKLKLPQVMGELVA
                     GILLGPSCLERCAPGFYESFFASPENAGVAYDGLARIGILLLLFVAGMEINLKTIQKK
                     ANAAAAISLSGVAFPFLIGFVASWYLSSYLFPQGMGDKLASSLFMGTALSITALSVLA
                     KILIDIDKIKSKFGNLMMTAAMIDDFIGWMLFSLVVTVANLESESSFQGWQVMFMVIG
                     FATFILTVGRRIIHWMFIFSNRYLSRGGGTLSVGIILCIFGAVFTEWLGLHAIFGAFL
                     VGIAVGDSEHFSHKNREMLHDIVTYIFAPLFFVSIGFRVDFIKNFDGGVIAFVLLIAI
                     LGKMVGGYIGGRLGKFSHNESIAIGFGMNARGSQEIVLGLIALNANLITDQIFVALVV
                     MTFVTIMIAGPSIRYFLQQHQEDDDEDEHNLTTAE"
     gene            18130..18627
                     /locus_tag="PLM90_04250"
     CDS             18130..18627
                     /locus_tag="PLM90_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88610.1"
                     /translation="MKGIMSAFLIVVGSLQAGATEVLEDTSFQRTNPKLLYTTRDYFV
                     NIPDNCMSDELCVKDFSFILTFNVGINTAQEIKQIGFKEADLDFMGKTAAAQTYKPHT
                     FTKYTSSSTNNGYSFTIDFIPDKAEGKMPVIEKFVFSGTITSGNLSGIMRFQTKNGVV
                     EVPLN"
     gene            complement(18624..19859)
                     /gene="rocD"
                     /locus_tag="PLM90_04255"
     CDS             complement(18624..19859)
                     /gene="rocD"
                     /locus_tag="PLM90_04255"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HMZ88611.1"
                     /translation="MTSNTIVSAKTQQYIDQESKFGAHNYHPIPVVIEKAAGVSVWDV
                     DGKHYFDFLSAYSAVNQGHCHPRIIAAMTEQAKKLTLTSRAFHNNLLGPFEAYMTELF
                     GYDKLLPMNSGAEAVETALKLCRRWGYEVKGIAEGKAKILVCEGNFHGRTTGIISFSN
                     DPDSTGGFGPYMPGYQIIPYNNLSALTEALRDPDVAGFLVEPIQGEAGVYVPDQGYLS
                     GAYAMCKDANVLFIGDEIQTGLARTGKMLACDHEDVRPDILILGKALSGGTMPVSAVL
                     ADDEIMLTIQPGQHGSTFGGNPLACAVAMTAMQVLVEEKLAENAEVMGAMLREALSGM
                     NSDYIKVVRGKGLLNAIVVNDNYNNKSAWDFCLALMENGLLAKPTHGDKIRFAPPLVI
                     TKEEMQTCIEIIRKTVNEF"
     gene            20111..20497
                     /locus_tag="PLM90_04260"
     CDS             20111..20497
                     /locus_tag="PLM90_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HMZ88612.1"
                     /translation="MKKSHIILLVLLALCIGVFASKLGNVSSYSSYKEAKEKEGKSVQ
                     LVGNLNKEKPVNFDPVKDPNSFSFTLIDKNGDNVEVVCYDDMPTDFEKSEDIVLTGAM
                     KGEVFYANEMLVKCPSKYQDNEITKQ"
     gene            20506..23007
                     /gene="ccsA"
                     /locus_tag="PLM90_04265"
     CDS             20506..23007
                     /gene="ccsA"
                     /locus_tag="PLM90_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMZ88613.1"
                     /translation="MGESHQHAIQEVNPIFGQIGQVLIITAFVAALLATCYYFRATRS
                     QQQIGENDNRKIARAFFIGHALSIVGVVVTMFIMIFNHHFEYHYVWSHSSSDLQMKYI
                     LSCFWEGQEGSFLLWTFWHVVIGLVVMRTSKEWETPVMSIVGLMQVFLLTFVLGIYVG
                     SWHIGSSPFLLLKDKLTADPVFLFNDYMRFIPDGRGLNALLQNYWMVIHPPVLFCGFA
                     SITFPFAFALAGLWKGKYTEWIKPAMPYALFAGMVLGTGILMGGAWAYESLSFGGFWA
                     WDPVENASLIPWLIIVAGLHNMLIAKKTGQALRSTFVMIFLAFFFVLYSSFLTRSGIL
                     GDTSVHAFVEEGLTNHLLLFVGVFFFLSFIPLIRKWKHIPAPEREEKLNSREFWMFVG
                     SLVLLFGGLHVIFFTSIPVFNTLITHVNDILGTSIRNDLGTPDDPIHFYSGVQIWVGV
                     LIAMLSAFAQYLRYKNSDGAGFVKRISYSFIFAIAATVISSIMLDKLWHITSERGVFH
                     LITSYSILLFAAWFSVIANAQYFIQVLKGKIRVGGGSVAHIGFGILLLGILISNAGQR
                     VISLNTGDVRFDAQGMDDTFQRENVRLIKNVPYPITDYNVTYLGDTLIAGATYYDLLF
                     TKTDPETGELMQQFTVHPYLLMDTKMQQLAPNPDTKHYLTYDVFTHVTSLPGEGSKNA
                     PPTTTLDTIGVGDTLHFDTGYMILKDLKNTSFSADSNEVKAFFDVHAGIASESIEVPF
                     IVSGRTMQVLQGTANVSVANVQVSFITILPDQSKKFVFKVDDSSLNTDWIVIKSIIFP
                     MINLVWIGVIVMAFGFFMSMLARLRERKEKSVVNG"
     gene            23000..23782
                     /locus_tag="PLM90_04270"
     CDS             23000..23782
                     /locus_tag="PLM90_04270"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HMZ88614.1"
                     /translation="MDEIKSILLVGTGNAAWHLGLNFKLNGVLIAGIVARDVEKGIAL
                     SAALGDVPVYGLEDKLPEVDATILLVRDDAVVEVAGKVAQEGKLLVHCSGSIKAEAIK
                     SGEHAFAVAWPLQTLQKDHPRPLFNTVVAVTGSDAETEQLVAGLFGKISNRVHVMSEE
                     QRSVMHMCAVWINNYTNHMYYIGHSLLKEKGLEFSMFFPIIEEHVQLLHEQDPEKLQT
                     GPAKRGDMGTLSRHMALLKDHPEWQDLYNQLSGSILKTYLKK"
     gene            23804..24133
                     /locus_tag="PLM90_04275"
     CDS             23804..24133
                     /locus_tag="PLM90_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ88615.1"
                     /translation="MVTIKFTFEDGSEAKEVQVEEGYSVLEAALLNNIKLNHNCGGVC
                     ACSTCHVYIEKGMEALPETTEKEEDFIDRARDPRLNSRLSCQCDLMEDGTFIEVLVPD
                     QSTIIAS"
     gene            24151..24390
                     /gene="iscX"
                     /locus_tag="PLM90_04280"
     CDS             24151..24390
                     /gene="iscX"
                     /locus_tag="PLM90_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HMZ88616.1"
                     /translation="MSQFEEPPIYWNDHEDIALKLYERFGDDFNESKIYRIRFTELIE
                     WVLEIPDFKGTREECNEGHLEMVQSAWVYEWRDNQ"
     gene            24445..25377
                     /locus_tag="PLM90_04285"
     CDS             24445..25377
                     /locus_tag="PLM90_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HMZ88617.1"
                     /translation="MKDWIRIIRPVNLLLLAATLYAIDGFILQPNFNTYGIRFSLNEF
                     QYFLLVISIVLICAGGYILNDYFDVQIDEVNKPSKVMIGKGIAPNSAFYVYMLLSFGG
                     LAIGTYLSLAVGNWKLVTIYVMAIALLYFYSATFKRYPLIGNMMVALLAGISILMTFI
                     YEPNLYQLARPGDYFIAGICTKFIIALGVIAFTSTMVREIVKDLEDMEGDDRYGAKTM
                     PIAWGINIAKVITVVFVLLTLLGIGYIFTNIIAAEGIIYGAYIGVMLGFWVAVSVRLF
                     LSRTAKQYHTLSTMIKIGMLIGLLIMPLYSIVEF"
     gene            25379..25942
                     /locus_tag="PLM90_04290"
     CDS             25379..25942
                     /locus_tag="PLM90_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HMZ88618.1"
                     /translation="MKIILASGSPRRSHILSTAGFNFTVMVPDVEETFPDSMPVMDVP
                     LFIADKKLQAVLTDTSVDNIVITADTVVILQDRIIGKPIDEADAFQILKSLSGNMHLV
                     VSGVCIAYQGKQYDIEVTTKVYFDHLTDEEISKYLQECKPFDKAGAYAIQEWIGMNKI
                     TRIEGDYYNVVGFPMSKIYPVLTALME"
     gene            complement(25943..>26227)
                     /locus_tag="PLM90_04295"
     CDS             complement(25943..>26227)
                     /locus_tag="PLM90_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ88619.1"
                     /translation="YARQQLSAIPHIHLQDRGARLSAIISLTSDRSDPMYFKEILNRN
                     GINAGAAARKFALLDFDQKGVSGAVRISPHYYNTFEEVDKMIAVIKENTI"
BASE COUNT         7395 a         5392 c         5398 g         8042 t
ORIGIN      
        1 attttaggaa gctgagcctt gcaagtttct gtaccactta cctgcaatgg aacgataagt
       61 tcctgtaagg gatttaaata agcatgcacc atttcgtggt tgttgcccat ttgttcacag
      121 tggtttacga tataaatcag gtcttcaact agttcgctgc ctagagcctg gtaagccgca
      181 ctatctgcat catcaggaaa cttgttcagt tgaacaatca tggaatcaat accctccgta
      241 gtttcaatat tcgatttcca ccgtttgcca ttgtctaaat ataaggctga acccctgcaa
      301 aggatatact tattctccgg agcctgcaga taaacagcat catcctgacg gcaagctatc
      361 aatgtgagtc ctaaagcaaa gaatcctaat ataacaggag gtagccgcat ggcggtataa
      421 aattagatgc ctttttaact ttcaggcaat gatttaaagc cctttgcaat ttttttacag
      481 tccttttgat gaacaattga ttaaacatga cgttatacta ctaatttact cgaataatga
      541 acgacaactc actactctgg cttggcttta atgcttttgt attgctcatg ttggcactcg
      601 atttgggtgt ttttcacaga aaatcacatg tagtcggcat caaagaggca ctcgcgtggt
      661 cgggcgtgtg gattgccctc gcattggtat ttaatggcct aatttatttt ggaatagttg
      721 gtggttatga aggtgctgaa ttacagaagc agaaagggat ggaattcctt accggttatc
      781 tcattgaaaa gtcgctgagt atagacaata ttttcgtctt cctgttgatt tttaccttct
      841 ttaaggtgcc acaattatat caacataaag tcctgttttg gggaattatt ggtgccttgg
      901 tgatgcgtgc aattttcatt ttcgcaggtg ttgctttgat agagaaattc cactggataa
      961 tttatgtgtt cggcgtcttc ctgatcatca ccggtattaa catggtgcgc aacaaaaaca
     1021 aacaattaga tccggagaaa agtcttgtca ttaaagggtt taggaaaata tttccggtgc
     1081 acaacagtta cgacgggagt aaattttttg tgacacttaa tggtaaaagg cacgctactt
     1141 tattatttgt agttttggtt tttatcgaat tcaccgattt gatttttgca gtcgacagta
     1201 ttcccgccat tcttgccatt acgccggatc cttttatagt atatacttcg aatgtatttg
     1261 caattctagg gttgcgcagt ttatattttg ctcttgcagg gatcatgcat cgatttgcat
     1321 atctggtgta tggattagcc gctgttcttg tttttgttgg tttgaaaatg gttttggtag
     1381 atgtttataa aatacccgtg caatattctc tgcttgtaat agccatatta cttgcagcgt
     1441 caattttcta ttctatatat aacacaagga aaatgaaagc ctgaattact gatgctattc
     1501 cacatcagat ttgatcccac gcaccttgag aaacccaatt gcaattattg cacagaaaat
     1561 accaaaggtc caccataatg catcgaatcc aagtttgtcg atgatctgtg tgctgcaata
     1621 gggtgaaacg ataaacgcaa ccgaccatgc gatggtatag gctgccaaat gtgagccacg
     1681 tgtttgtggg gttgccgttt tcactacatg tgatatcata aatggcatgg caaatatttc
     1741 tgaaaaactc agtaaggtca ttgcagtgat taatacccac atgccggtga ataaattgaa
     1801 aatggcaaat gaaacgccaa gcatcaaggt tcctgcaata atgagccgct caggtttaaa
     1861 tcgcttttct atttgcgata ccgtgatcat ttcaaataaa aatacaatca cgccattaaa
     1921 tccaattaat aagccgatag ctttttctga ttgaaaatat acgtctttgt aatataaggg
     1981 tatgctgcta aaaatctgaa aaaacgcggc tgcataacaa aaacataaaa atagaaaata
     2041 caaaaatgca ggttctgtat acggcgattt ctgtggttga tcttcctgtt tatcttcttt
     2101 atgaatttgt ggccttttac ctctgaaata aaagaaaaac acaactcctg cagcaataca
     2161 agtaatacca tcaccaaaaa acaaccagaa ataatttgtt tctgccaata atccggcaac
     2221 tgcaggacca atagccacac ccagattgat tgccattcgc attaatgaaa acgaacgagt
     2281 tgttgtttcc ttggtagcaa aatgtccaat catcgatgac gctgcagggc gcatggcatc
     2341 attcacagtt gcacaaacaa aaatccctaa agccagcgaa taaaatccgg tcagcaatgg
     2401 caataataaa taacacccac ccccaaataa caaactgaat aactgcactt gaaatggccc
     2461 gaatttatct gtccacctgc cacctaaata cgatccaagc aatccgccta aaccatatac
     2521 cgacatgatt attcctgcct gcacagtgct gtatcctaaa ttggcagtca tataaactgt
     2581 cataaaaggc aaaaccatgc tgccactgcg gtttatcaat tgaatggcag ccagtatcca
     2641 aacagatctg tttattcccc tataggaatc aagataaata ttgagcgcct tgccaaacac
     2701 agcaacgcga aggtattata attaagaatg aagaaaatct gattggcgtt gataatcaac
     2761 tacgaagggt cattccatcg atacatcggg actgaaaagt ctgctaattg aagtatcctg
     2821 tcaacaacag tagcagctac ctcttcctga gatttcggtt tgctgtaaaa tgagggcgac
     2881 aatgggcaga tgataccgcc ggctgtagta accgtttcca tatttctgat gtggatcagg
     2941 ttgtaagggg tttcacgcac cgctaaaata agtttcctgc gctcctttaa catcacatcg
     3001 gcagtgcggg tgagcaaatc attggaaatc ccggatgcaa ttctgccaag tgttcccatg
     3061 gaacagggac aaacaatcaa cgtatcaaac caggctgagc cactggcaaa aggtgccatg
     3121 aagtcgtttt tgccataaaa agtgaaaggg taattcgtaa attgctcatt atcaagctca
     3181 tagcgccaaa catccttagc attatcgctc ataactacgc caactttctc tatttcagca
     3241 cctaaaagct gtagcttgtc cagcaaaact ttggcataaa tggcaccact tgccccggta
     3301 attcctactg ctatcttcct ttttctcatc tttgtccgcg caattccatt gcaagttaac
     3361 cactttggaa ttatttttgt ttacaggtag ttgaacgaat caatactatg caaaagagaa
     3421 tactgatttc tgcagcctta attatgctgc ttggtgccgc actgatggct tttgtgccga
     3481 tggacacact taaggaagcg tcaggtgcag atttaatgat ggctaccacc gttgaagatt
     3541 ctgccaaaac tgaattcaaa tggtacacga tttccgaagc attggaaatg cagaaaacat
     3601 cgcacaaaaa gatttttatg gatgtttaca ctacctggtg tggaccatgc cgcatgttag
     3661 acgcaaatac cttttctaac cccgaaatcc aaaaactgtt gtcggaatac tatattcccg
     3721 taaaattcaa tgctgaaagc ggggatacga ttcaatacca gggtcaaacc ttcacaaatc
     3781 gcaactatag tccactaccg cgcaagagta ctcatgattt tgcagtgtat atcgcttcta
     3841 cagagcaggg attaggttat cctaccgtag tattcatgga tgaaacgggt actcgtcttc
     3901 aaccaataag cggttatcgc gaagcaggta agggaacagc tcaaaatccg ggaatggaac
     3961 caattttatc gttttttggc acagatgctt acaaaacggc cacctggaac gatttttatg
     4021 ctgcatttca gtctaatatt aagtaatcca tgtttattga aatagcactt tcgcttgtga
     4081 tgttcttcca tgaggatcat caagaaacta ttgctcctgc tgcagaacag gctggtgttg
     4141 ataccattgg atggatgtca ctttccgatg tcaacaaaat tgttgaaaag aacatcaaaa
     4201 agggtaaggt aaaggaggat aaactggtaa tagtggattt ctacacagac tggtgcggct
     4261 ggtgcaagaa acttgacgct gaaacgtata aggatccgga agtcgcagct ttgatgaata
     4321 aatactttta tccggtaaaa tttgatgccg aatctaaaga taccgttgct tttgccaata
     4381 agacttatgg ttttaaaagc tccggcagcc gaggcactaa tgaattcgcc ttggaaatgg
     4441 caagcaggcc gggcggtaga atagggtatc caacaattac catcatcaat gctttgggag
     4501 aaaagctgac cgtagaagca ggctataaaa atcccgaaaa aatgaaattg atgctgctgt
     4561 attacggtga aggacactat aaaacgaagg acttcaacac ttttgtcacc gaatacaatc
     4621 aaaaacaact tactgattaa gaggttctgc agaaatagcg cataaacagg tgcataatat
     4681 ttacgggatt cctttcacga tataatggct agggttaact ttgcccggtt tacaatatgt
     4741 cctcagcagc aaaaaatatc cataagctat cagctgccgg cgtactgatc tccctgggaa
     4801 tcatttttgg cgatatcgga acttcgccaa tctatgtgat cagtgctatt gtcggcgatc
     4861 atgtcatttc gcgcgagtta atccttggcg gcttgagttg tgtattctgg acactcgtca
     4921 tcatcaccac attcaaatac gtcattcttg ccctgaacaa cgataatagg ggagaaggag
     4981 ggatatttgc cctttatgcc attcatcgaa ggtttaaggt taaatgggct attgttcctg
     5041 cattggttgg atgtgctgcc ttgattgctg acggatttat tacaccccca atttctatct
     5101 cctctgcaat tgaaggtcta aaactgctaa acccgaacat cgattctgtt gtgccgattg
     5161 taattattgc catgatcatc ttgttcaccg tgcaacaatt cggtaccaat gtggttggaa
     5221 aggctttcgg tccggtgatg tcgatttggt ttttaatgat cggtattctg ggttctattc
     5281 aagttgtgca acatcccgaa gtgcttgagg ctctaaatcc atactgggca atccatttac
     5341 tcacagaata taaaggaggc ttttggctac tcggaagtgt ctttctctgt actacaggtg
     5401 ccgaggctat gtatagcgat ttgggacatt gtggtaaaca aaatatccga ttctcatgga
     5461 catttgtgct taccatgctg ttgttgaatt atgccggaca gtcggcttac atgttaacca
     5521 tggatggtca gcaactcaac gaccatagtc cgttttatat gattatgcca acatggttcc
     5581 ttcccatcgg aattattgtt gctaccagtg ctaccatgat tgccagtcag gcaattctca
     5641 caggttgttt tacgctggtg aatgaagcca tgaaattaaa actgtggacc aattttaaag
     5701 tggtataccc ttcaaccgtt aaaggtcaga tttatattcc tgccatcaac tggtttttgt
     5761 ttgccggttg cgttaccgta gtactgttat ttcgcgaggc aaaacatatg gaagcagcct
     5821 acggtttggc catcactttt gatatgatca tgaccacggt tttgcttacc agcttaatgt
     5881 tattacacaa gcgagcaaaa ctcatggtgt tcagcctcgc agcattgttt ttgttgatag
     5941 aagggtcatt cttagtgtca aatctcaata agtttacgca tggcggttgg tttaccgtgt
     6001 cgcttgcagt tggtattttc tttgctctgt ggattttcta cgaggcaagg aaattacgcg
     6061 ccaagcatac cgaatttgtt gagatcgata aatatctccc tgctattcag gatttgatgc
     6121 acgatgaatc cattgctaaa gaagcaacgc atctggttta cctgagtatg gtgaacgata
     6181 agaatcatat cgatagtaat atcatctatt ctatattccg aaagaaacct aagcgtgccg
     6241 atgtatattg gtttgtgcac gtagatattt caaatgatcc ttatctggca acgtattcgg
     6301 tagataccat tgtgccgaag aagatcttct ttgtgcgatt gcggttcgga tttaaagtag
     6361 aacacaaagt gaatgtcatg ttccgtcgcg tggtggaaga aatggttgct gcaggtgaag
     6421 tagacttgct gagcaattat cctagcctcc gcaaacatgg catgtatgcc gatttcaaat
     6481 tcatcctgct caactcacgc gtttccattg ataataaatt atcaccatgg gaacagatcg
     6541 taattaaagg atacagattc ttcaaatcgc tgtcgttgcc aacacacgag gattttggtc
     6601 tggaactcac caacgtagaa tacgagatgg ttccgattaa agtaagtcag gaaatcgaga
     6661 ttaaactcga acgcgttaca aagtagattc ttcgctggct acgggtttct caatacgatc
     6721 aacattcttt tggaatatcg gacgcatggt gtcaagatct aaggcacaaa ggtgtccata
     6781 tcccgggcga ccaacataaa cgcatccgca atcgatgttt acggcaggat atttcatttc
     6841 actcaactcc tgttcaagcg tgttgaatgg tctgctggta tgtccatgca caatgatctt
     6901 attattaatt ttttcaggga tgatggtatc gtagtaattg cgaatccaat aaagactgtg
     6961 agtatccttg aaaatatctt cttccttaaa gttaaatcct gcgtgaacca aaatcacctt
     7021 gtctaattca atataaggct tcaagctgcg cacccaggta tcatatttct tatcaatgtt
     7081 tttcagatca tcaatacgtt tgtaacctaa attcatcatg gttgttacac cgccattttt
     7141 cacccaagcc tgatagcgac gccagtccca gcaatatgga atagtttcat ccaacgcttg
     7201 cataaacatc cattcgtgat tccccatcaa ggttgtcact ttgtagcctt cttcaaccat
     7261 tttaagcacc tgatcaatga ctgcaatgct atttggtccg cgatcaatca aatcgccgag
     7321 gatgtataat tcgtcctctt tggtcaattg taaggtgtca agaagggctg aaaatgtctg
     7381 gtgacagcca tgaatatcgg aaattgcaaa tctgcgcata ggtctaattc agttttaaat
     7441 tgttgagaca cagcacctta agtttcgaaa catcggggat tgtgccttca gaaatatcct
     7501 gcaaagatat ttgttttggg gtattgggca acaggtcgac gaagttattg ctcagtctga
     7561 gttctgtatc gtcgctgtag aggtagactt gtcgggcaaa tactggactt gtcaatgtaa
     7621 taacatcacc atcgatactg atagctatat ctgctttttt gtagctgaca gcacccggcc
     7681 ttacaaaggt gtgcattcgt tccgcaatca ccttgtcttc ctcactcagc gatagccaaa
     7741 gaatacaatt ttcctttgca tatccgctca aaaaccattc aactcctaat gttgaatata
     7801 acatggaagt atttgcctta agtgtaaccg gtaaagtctg attgcgaatg gtaacgcctg
     7861 atgttgttaa taatgaatag gtcaattgca attttctgtc agtagtatca ttatttacca
     7921 accatgtttg caattcatct ccttcctgta aaacactgat tatcacatcg cgatagcatg
     7981 actcaacagt ataccaggca gcttttctat tcccataaaa atcttgcaca ctccagcttg
     8041 ttacaggcca acaatcattc aactgccaaa acatcgttcc catgcaataa ggcatatttc
     8101 gacgatgcgc ttcaatcgct acacgcatgc catcagcttg caacaattgc gaaatgtaaa
     8161 tgtaggattg caggtcagat ggcgtgccat aatcgcgctt catatattca tcgatggttt
     8221 cataaccgac gggatgtttt tgatgcgctt taaaggccgg agagccaaga tataaattgt
     8281 ctgaattaat acatttttcc caactatgat atgagggtat gccttgaaat ccatattcac
     8341 tcataaatcg gggcaccttt ttattataga tggcaaatgg ttgtttaccc caccaaacgc
     8401 cccagtagtg tgcatctccg gaagtcagac tttcttttcg tccccagccg taagtaggtg
     8461 aggagggatg ataactttgg tacgcaccgg catttgaatg taaatactca ggaatcacta
     8521 atcgaaataa cttctcgttc aacgaagcaa cataggccga atcatcatcg ttgtatttca
     8581 attgtttttg ccatccccag ttattccatc cttcatcaat ttcattattg ccacaccata
     8641 atgctaaaca ggggtgattt ttgaggcgcg ttatctgttg ttgaaattcg ggatattccg
     8701 ttacgtcatt cggatacatg gcgcaagcaa acataaaatc ctgccatacc attattccca
     8761 atgaatcgca aagatcataa aaaatatcct gctcataaat gccacctccc caaacgcgga
     8821 tcatattgat atgcatgcgc tttgcttcta ttaaaatatc gcgataacgc tcatctgatg
     8881 cttctgttgg gaaactatgt gcaggaataa aattcgcgcc atttatgaag acctttttac
     8941 cattcgcagt aaaataaaat gacttaccac tcgcatccgc ttcctgatgt aattgaatgt
     9001 tactaaatcc cgttttgaat acctgctgat aataaatgcg tttgttatgt gtgattttta
     9061 attcaaatgc ataaatattt tgagatccta atgcataggg catccaaact tcaggagcat
     9121 ggaaattgaa attgatttgt tcaaccgtag gcaattgatc tctataactc cagctatagc
     9181 aggttttgcc tgtagtgagt tctttcactt caacctcgta atgcatacga cctaaagctt
     9241 catatgccag atcaatatgc acagggaact ggtaatcagg attttgttcg gtataaaaat
     9301 caacaaactt gattttcaat ttatcattaa aaacaagtgt tataggctgc caaatgcctg
     9361 aagttaccaa acgcggtccc cagtcccaac cgaattgata ttgtgccttt cggatatata
     9421 cgcgctcgtc gcttggtagt ggcaccgtaa attgttcacc aacttcgttc gctcgtttta
     9481 ccggcgaata gaaatgaatg aataacagat tatcgccact tttcaaaaga gtcttcaccg
     9541 gcactttata tgctcgaaac atatttgttg atgacaatat cagtgagtca ttcaaataca
     9601 catctgcata agtgtcaagt ccgccgaaat tgagatcaat gaaatcactg tttaattgat
     9661 ttttatgtag atgaaatgta gttcggtatt cccaatcttc attctctacc cattgcacag
     9721 ctgcttcatt tgtgtcgatg aaaggatcag ggatggcatt atttgttaga agatcagtat
     9781 gcacacatcc gggcactttg gctttcatcc attgtgtatc gccaaagcga cgaaattgcc
     9841 actgttgatc aagtgttata ctttccattt gcgcatgaac agaaaaatgt agcatagaac
     9901 atgccaatat caaaaggtgt atactcttca tgcgttatat gtagcaataa tggcacttaa
     9961 tttgcgtacc tcttccggat ttacttcggt aagatgtgag gtgtcgtaaa tgtttgaatt
    10021 aaagttatag gaagttgact tgggtgtgaa tggtcgcttt gcagaagtgt ggatttcgtt
    10081 gcaaccggta tagtcaataa tttcacgtgc attactgcta ttaacgcctg ctcccggaag
    10141 taaagtaatg ttattgtttg ccgatgtaat caattgcttg attatttcct tgccttcaat
    10201 tgcggtgttt gcctgacctg aagtgagtat tcttgtaaat ccacaggtaa tggcatcttc
    10261 taatgcttca aagggatcgc gtgtaaggtc aaaggctcgt tgcaatgtgc attgcatacc
    10321 tgacgcagta tcaataagcc gcgaatttct ttctacatcc aactttccat caggtgtggt
    10381 gatgccaaat atcacttgtt taacgccaag ggagcgacaa tgaagtatat catgacgcat
    10441 cacatcaaat tcaaaatcgt cgtacatgaa atcgccacct cgggggcgta tcatgacgac
    10501 aatattttcc ggatctatca tttgcatggc agcagtgata gctgcagaag aaggagtgat
    10561 gccgccttca acataacttg cacataattc aattctatcg gcgcctgcag caactgcatg
    10621 cacaacatca gtggcattga atgtgcatat ttccagtata tactttcttg tgactgatgt
    10681 tttcatttat tgctcggggt aatagctgtc tgatttgaat agttcgttgg caatattatt
    10741 cgaaaacaca gcataattaa atccgggttg caggcaataa gcgccaaatt caccagcttt
    10801 gttcaatgcg atgaaaccga cctgaaaatc tttagttttt tctttgcgga tctttaccat
    10861 acgcatcacg gcttctttgc atgcttcgta tggcgagtat ttctgccgca ttaattccac
    10921 taccagatgc gtacctgcaa ttctgatcac ttcttctccc gttccggttg ctgttgcagc
    10981 gccgatttca ttatctacat acaatccggc accaatgata ggtgaatcac ccacacgacc
    11041 acgcacttta taagcaagac cgctggtagt acacgcaccg gataaattac cttgcatatc
    11101 catggctatc atcccaatgg tatcgtgatt ttcaatattt atgacaggtt tatattgttt
    11161 ttcaacaagc catgcttcat atgctttttg tgcttccgga tttttatagt cgtcaagtgt
    11221 gaatccattt tccagtgcaa attgttgtgc tcccgaacca acaagcataa tatgtggtgt
    11281 tttttccatt accatgcgcg caacagaaat cggatttttt attttctcta aaaacgccac
    11341 gcttccgcaa ttgtagtgct catccatgat acaggcatcc agcgtagtat gtccttctcg
    11401 gtcaggtaat cctgcaagtc ctactgaaag agaatgcaaa tcattttcag caactttcac
    11461 accggcttca accgcgtcaa gtgcgcgacc tttggcacta agtatagccc atgcatcaac
    11521 atttgcttta agaccaaaat cccatgtgga taataccaag ggtttattca tgggaactga
    11581 cttgtgctga ttttcaacta cggcagattg cagtgactgg ccggtgctga tgacgcctgc
    11641 tccaagggct gcagttttta taaacgatct tctggatgtc atgcaacaaa tatcggaaat
    11701 gcatttgata gcaggaagac ggcgtctttt gcaaattgct ttcagactac atgaatggtg
    11761 tcatctgata tgaacctcat cgacaaacaa ccatgctttg tttccggccc cggggtttcc
    11821 ttgaggaatt gtgccgatat tatttgcgat tatcctgaca tatcttgcgg cagtaggatc
    11881 gaacttataa atgccattcg ttattgcacg atcatccttt accacaggca caattccgat
    11941 ccgagtgaat ttttttccat caaggctcac cgatatttcc atggaagacg gaagatatat
    12001 ccaactggtt ttgtcttcca aaaacccggc acttactgcc atgatagtat ctacagctcc
    12061 aaggtcgatg gtgcaattta aattcgttcc gctgaatccc agccattcat tgccactcca
    12121 tgatggatat aatgatgcat taattccgtc aacaagtgaa aaggctccac ttccggagta
    12181 ttgttgtgca ggtggtgttt tgagagttat tttttttcct gttgccttgt tgaacaataa
    12241 acgcaatttt acttcaggat gtttatagtc ttcaggaata ataagagaat aagattcatt
    12301 tacaataaat ggtgctgtat aaattccttt tgaaaaagct cctccaaaaa ctgcaggaga
    12361 atttttagaa gtgtcctcat attggtattc gaatcctgtt gtgttatccc aggttcgtaa
    12421 agctatacga accccgttaa aatcatcggt aggtaatact tcgggaatga taccgaatgc
    12481 acttttgcta taatggcaat ctactcgtcc aagcaactca aattgagtag acaatcggcg
    12541 cataaaatca gcataattgc gggccgtatc aggtgtccat aatacctcac taagagctaa
    12601 catgcgtggt attaccatat attcaacctg atcgaaattc ggaatatatt cagtccataa
    12661 atttccctgt gcacctagaa tatattttga ttcctcaggc gtaagtgcat cgggaatggg
    12721 atgatattca tatacctttt gcaatggaat atatccgccg attgcaagtg gttcctgata
    12781 gggtagtccc tggtaataat cgaaatagca atgcgatccg ggagtcatta ctgcatagtg
    12841 ccctgcacgt gcggcatcaa ttcctccatt tacacctcgc caactcataa tggcagcttg
    12901 ttgcggaaga tcattcgtaa gaatttcatc ccagcctata atattgcgcc ctttgctttg
    12961 cacatactcg gcaattgttt ttgtaaaata tccttgcaat ccatgttcgt caactagatt
    13021 tttttcttta atcaattgct gacaataatc gcaatgcatc cattgatctt tctctacttc
    13081 atcgcctcca atatgtatat attgagaagg aaataatgcc atcacttcgt ctaacacacg
    13141 atataaaaat tcaaacgttt ccggtttcgg acaaaatgct tctttataca ctccccatgc
    13201 ctgacctact gaatatgttg tatctgcaat gcaacctaat tcaggataag ctgctaaggc
    13261 tgcagcacag tgtccgggca tttctatttc aggtatgatg gtaatatttc tgtttttcgc
    13321 gtacttcaca acctcagaaa tttctttctg tgtataataa ccaccataat taatcgtgtc
    13381 gtattgttga tcgcgataat gacctttcat ggtgccggtg cgataagcgc ctacagaagt
    13441 taatttagga tattgtttga tctcaatacg ccaaccctga tcatcggtaa gatgccagtg
    13501 aaaggtgttc attttgtata atgcgagata atcgatgtac attttaactt catccaccgt
    13561 aaagaaatgt ctgctgcaat ccaggtgcat gccgcgatat tggaattgcg gaaaatctga
    13621 aatgcctgcg catggaacct gaaactgatt ggtgaatttt gattcagtca tttgaagtaa
    13681 tgacatcaac ccataaaaaa ctccgccttt gttttttccg atgatcgtga ttttttcttc
    13741 ggtgatgagc atggtataac caccatccgg aatcattaaa gtactgtcgt aggatacttc
    13801 aatataatgt gaaggataaa tttcttcgcg aactgttttt aatggcagtg aatgaaattt
    13861 aatactcatg tcacggaacg taacagcatc cataaaagat gccatgtcgt tcaccaaaat
    13921 ataactggta ggcttaaaat aaaaatatcc ggggttggcg gtgtactcaa caggtttagg
    13981 cacaatgggg cattgctgtg ctttcaacat gtttacatga agtgtcagaa gcaaggtaaa
    14041 aattagtgtg gttttcattg cttctaatta aacctgcata acgccaacgg aaaaatcttt
    14101 ttcgataggt gcattatttg ccatttcaat tcccatgctc acccattttc tggtatcaag
    14161 cgggtcaata atgccgtcga tccataatct ggctgccgca taatatggat ccatggtttc
    14221 attataatga tttaatattt tctcgagaag tgctttttct ttttcagctg taatctcttc
    14281 gcctttagct ttcagcgatg caacctgaat ttgcaataac acttttcccg cctgagcacc
    14341 gcccattacg gcaatttttg cattaggcca cgcaacgatt aagcgcggat catatgcctt
    14401 tccacacatc gcataatttc ctgctccaaa actattgcct atgattactg tgaatttcgg
    14461 gacgacagaa tttgcaactg catttacaag cttagcgcca tctttaataa ttccggcatg
    14521 ttcactctta ctgcctacca taaatcctgt cacgtcctgt aaaaataaaa gtggaatttt
    14581 tttctgattg caattcatga taaaccgtgc agccttatca gcactatcat tataaatcac
    14641 acccccaatc tgtatttctc cttttttagt tttcaccacc ttgcgctgat tggctactat
    14701 gccaacactc cagccatcta tcctcgctgt gccgcaaaca atggtcttgc cataagcggc
    14761 tttatattcg tcgaatacag aattatctac tagacgttta atgatatcta atacatcata
    14821 ttgcttggtg atatcctcag gcatgatgcc atatatatct tcttctttta aggatggtgc
    14881 agatggtgca atgcgatcaa atcctgcttt tggcatagcg ccgatcttat ccatcgtgct
    14941 gcgaatatgt gtgatgcaat cttcatcatt ttctgcagca taatcgcaaa taccggaaat
    15001 ttccgtggtt gtaattgcac ctcccagcgt ttcagcatca atatcttcac caattgctgc
    15061 tttcactaaa taaggtcctg caagaaaaat gctgccggtg ttttttacaa tgtatgcttc
    15121 atcactcatt ataggcaaat aggccccgcc tgcaacacag ctacccataa ttgctgcaat
    15181 ttgtgtaatg cccattgaac tcatcacggc attgtttctg aaaatacgac cgaaattttc
    15241 cttgtccgga aaaatctcat cttgcatagg tagatataca ccggcactat cgaccaaata
    15301 aatgattggc aatttatttt ctatagaaat ctcttgagca cgtaaattct tttttccggt
    15361 tatcggaaac catgcacctg cttttacagt ggcatcatta gcaacaacaa tacactgcct
    15421 gcctgaaaca taacctatca ttactacaac tccgccgctg ggacatccac cgtgttcggt
    15481 gtacatgccc attgccgtaa aagcaccaat ttcaatacat ggtgtatcca tgtcgaaaag
    15541 taatttaata cgttcgcgtg catgcaactt tcctttttca tgcaatttgt caatggattt
    15601 ttttccacca ccttcctgca ctttcgcaag tctttgcttc atggtagata ccaataatcg
    15661 caatgcatct tcgtttctgt taaatgctat attctgagtt tctttcgtca tgtgtcagtt
    15721 ctgtatgctg caaaaataca attaccgcgg caaccgttgt aagatgtggt tttttaacta
    15781 cctttgcgcc gggcaagtcc tatacgacca gctcctgctg aatcccccag gccctgaccg
    15841 gagcaagggt aggcggttgt agcggtgcga tgtagatcac ttgcccattt tttttatacc
    15901 taagcctcat gttgaaaaat caggcttgcg gtgaacatcg ggtcacctat ttttgcaaaa
    15961 acacaacttc tactgatgaa attttttatc gatacggcag accttaatga aattaaagag
    16021 gctgcatctt tgggtattct tgatggcgta accaccaatc cttccttaat ggcgaaaatc
    16081 ggcattaccg gtgctgaaaa tattaaacaa cattatatcg acatctgcaa tattgttgat
    16141 ggcgacatta gcgctgaggt tatctcgact gattttgaag gcatggtgcg cgaagctaaa
    16201 gaactggttg cactccaccc gaatattgtt gttaagatcc caatgatcaa agacggtgtc
    16261 aaagccctga aatggtgtac cgataataat atcagaacaa attgcaccct cgttttctcc
    16321 gccggtcagg ccatactcgc tgcaaaagcg ggtgctactt acctttcgcc atttattggt
    16381 cgtttggatg atatcaattg ggatggcgtt caattaattg agcaaatagt acatatctac
    16441 cgcaattacg gctatgaaac acaggtttta gccgcttcta tccgcaatcc gttgcatatt
    16501 attcgctgcg ctgaggttgg ggctgatgtg gttacctgtc cgcttagttc aataatggga
    16561 ttgttcaaac atccacttac cgatataggc ctggcgcagt tcctggccga ttacaagaag
    16621 ggtaacggtt gatcatatcg aaaaatctac taaagtatta gacttttagg cctgtctgac
    16681 tatatttgca aggcaggaat gatgtattgc ctctgttgac acaaggttag gttatccgtt
    16741 atcaacagca catccatttc ggcgttaaac catctaatac tatgcatcga ctcccatcgg
    16801 acgaggtctt gccattttta ttaatggtag ccactttgtt gcttgctgca cggctcatgg
    16861 gcgagttgtt tcgcaagttg aaactacctc aggtaatggg ggagttggtt gccggtatct
    16921 tgctgggacc ttcatgcctg gaacgttgcg cacccggttt ttatgaatct ttctttgcct
    16981 cacctgaaaa tgcaggtgtt gcttatgatg gacttgcccg tattggcatt ttactgctgt
    17041 tgtttgtggc cggaatggaa atcaacctga aaaccattca aaaaaaagcc aatgccgctg
    17101 ctgcaatcag cctttcaggg gtggcttttc cgtttttaat tggatttgta gcctcatggt
    17161 atctgtcaag ttaccttttc ccccaaggaa tgggcgataa actggcttcc tcactgttca
    17221 tgggaacagc actgtccata acggctcttt ctgtgctggc taaaatactt atcgatatag
    17281 ataagattaa aagcaaattc ggaaatctca tgatgaccgc tgccatgatc gacgatttca
    17341 tcggctggat gctgttttca ttagtagtta ccgttgctaa tctggagagc gaaagcagct
    17401 tccaaggctg gcaagtgatg tttatggtga tagggtttgc cacatttatc ctgactgttg
    17461 gccgccgcat tatccattgg atgtttatct tctccaatcg ttacctctcc cgcggaggtg
    17521 gcacgctttc tgtagggatt attttgtgca tcttcggcgc cgtgtttaca gaatggctgg
    17581 gacttcatgc cattttcgga gctttcctgg ttggtattgc cgttggtgat tcagaacatt
    17641 tcagccacaa gaatcgcgaa atgttgcacg atatcgtgac atatattttc gctccacttt
    17701 tcttcgtatc gattggattc agagtagatt tcatcaaaaa tttcgatggt ggggtaattg
    17761 ctttcgttct gcttattgcc atcttaggca aaatggtagg ggggtatatt ggtggacgat
    17821 tagggaaatt ctctcataat gaatccattg ccatcggttt tggcatgaat gcacgcggat
    17881 ctcaggaaat tgttcttgga ttaattgcat taaatgccaa tcttatcacc gatcagatat
    17941 ttgttgcctt agtggtgatg acctttgtaa ctatcatgat agcaggtcct tcgattcgct
    18001 atttcctgca gcaacatcag gaagatgatg atgaagatga acacaatttg acgacagccg
    18061 aatagcttat ccacatttag ccggaaatag aatggcacgg tatttcgtta tacattgcaa
    18121 agtcaaggta tgaaagggat aatgagtgca tttttaattg ttgtcggcag tctgcaagca
    18181 ggcgctaccg aggttttgga ggataccagc ttccagagaa ccaatcccaa attgctttac
    18241 accacacgcg attatttcgt taatattccg gacaattgca tgagtgatga attatgtgtg
    18301 aaagattttt cattcatcct cacattcaat gtaggcatta atactgccca ggaaatcaaa
    18361 caaattggtt ttaaagaagc tgaccttgat tttatgggga aaacagcagc agcgcagact
    18421 tataaaccac atacgtttac aaaatatacg tcgtcaagta ccaacaacgg atattctttt
    18481 accattgatt ttattcccga taaagcagag ggtaaaatgc cggtgataga aaaattcgtg
    18541 ttttccggta ctatcacatc cggtaattta tcaggtatca tgcggtttca aacgaaaaac
    18601 ggtgtagtag aggtccctct gaactagaac tcatttactg tttttcgaat gatctcgata
    18661 caagtttgca tttcctcttt ggtaattacc aatggaggag cgaatcgaat tttatcgcca
    18721 tgtgttggtt tagccaacaa tccgttttcc attaaggcca ggcaaaaatc ccatgctgat
    18781 ttgttgttgt aattatcatt caccacaatg gcattcaata aaccttttcc tctcacaact
    18841 ttgatataat cggaattcat tccactaagc gcttcgcgta acatagcacc cattacttcg
    18901 gcattttccg caagtttctc ttcaacaagc acttgcatag cagtcatggc aacagcacat
    18961 gcaaggggat ttcctccgaa cgttgatcca tgctgacctg gttgtatcgt caacatgatt
    19021 tcatcatccg ccaaaacagc acttacgggc atggttccac cactgagggc tttgcccaga
    19081 atgaggatat ccggacgaac atcttcatga tcgcaggcga gcatttttcc tgttctggca
    19141 agtccggtct ggatttcatc gcctataaac aacacatttg cgtccttgca catggcataa
    19201 gcgcctgaca aataaccttg atccggaaca tacacaccgg cctctccctg aattggctct
    19261 accagaaatc ctgcaacatc cggatcgcgc agtgcttcag tgagtgccga caagttattg
    19321 tatggaatga tctgataacc aggcatataa gggccaaaac ctcccgttga gtccggatca
    19381 ttgctgaatg atataatgcc tgtagttctt ccgtggaaat taccctcgca aacgagaatt
    19441 tttgcctttc cttctgcaat acctttcacc tcatagcccc agcgacgaca aagctttaag
    19501 gcagtttcaa ccgcttctgc tcctgaattc attggcaaca gcttgtcata gccgaataat
    19561 tcagtcatat aagcctcaaa cgggccaagt aaattattat gaaaggctct gctggttaat
    19621 gtcaacttct tcgcctgctc agtcatggct gcaatgatgc gtggatggca gtggccttga
    19681 tttacagctg aataagctga taggaagtcg aaatagtgct ttccgtcaac atcccaaact
    19741 gatacaccgg ctgctttttc aattacaaca ggtattggat ggtaattatg cgctccgaac
    19801 ttcgattcct ggtcaatata ttgctgggtt ttggccgata ctatggtatt agatgtcata
    19861 taatgtgact taaagtattg attatcaaat agttatgaaa aatgaaaaat agacatggag
    19921 atacaggaaa ccgctatcag acctgtggat ggtcgaggaa atcgaggttc atatccaaaa
    19981 agtgcatcat atcgcaacaa agatagccaa aaaaaggatg tgagataatc gttcacaaat
    20041 actgaagtaa tgccatgcta acagaacatt ttgggctacc tttgcgttga aaattataaa
    20101 ggttcaggta atgaaaaaat cgcatatcat attgttggta ctccttgcgc tttgtatagg
    20161 agtatttgcc tctaaactgg gcaatgtaag ctcatacagc agttacaagg aagccaagga
    20221 aaaagagggt aaatcagtgc agttggttgg caacctgaac aaagagaaac ccgtaaattt
    20281 tgatcctgtt aaagatccga atagtttctc attcaccctt atcgacaaaa atggtgataa
    20341 tgtagaagtg gtttgttatg acgacatgcc aacggatttt gaaaaaagtg aagatattgt
    20401 attgaccgga gctatgaagg gagaggtgtt ttatgccaat gaaatgttgg tgaaatgtcc
    20461 ttccaaatat caggataacg agattacgaa acagtaaaca cctgtatggg agaatcgcat
    20521 cagcacgcaa tacaggaagt caacccgatt ttcggacaga tcgggcaagt attaatcatc
    20581 accgcatttg tagcagcatt actcgcaacc tgttattatt tcagggcaac gagatcgcaa
    20641 cagcagattg gtgagaatga caatcgcaaa attgccagag cattttttat cggacatgca
    20701 ctctcaatag ttggagtggt ggttaccatg ttcattatga tattcaatca tcactttgaa
    20761 tatcattatg tatggtcgca ttccagcagt gatttgcaaa tgaaatatat cctttcctgt
    20821 ttttgggaag ggcaggaggg tagcttttta ttgtggacct tctggcacgt agtgataggc
    20881 ttagttgtga tgcgaacctc gaaagagtgg gaaacgcctg ttatgagtat cgtaggtttg
    20941 atgcaggtat ttttgctcac attcgtctta ggcatttatg tcggtagctg gcatataggt
    21001 agttcgccat ttttattgtt gaaagacaaa ctcactgccg atcctgtatt tctctttaat
    21061 gattacatgc gtttcattcc tgacggaaga gggttaaacg ctttactgca aaattattgg
    21121 atggtgatac atccgccggt attattttgt ggattcgcat ctattacatt tccatttgca
    21181 tttgcactgg caggtttgtg gaaaggaaaa tatactgaat ggataaaacc tgcaatgccc
    21241 tatgctcttt tcgcaggaat ggttttggga acaggaatat taatgggcgg tgcctgggct
    21301 tatgaatcat tgagttttgg cggcttctgg gcatgggacc ctgttgaaaa tgcatcgttg
    21361 atcccttggt tgattattgt tgccggatta cataatatgc taattgccaa aaaaaccgga
    21421 caggctttgc gttcaacatt tgtaatgata ttcctcgcgt ttttctttgt gctttattca
    21481 tcatttctca cacgcagcgg aatccttggc gacaccagcg tgcatgcatt tgttgaagaa
    21541 ggtttaacga atcacttgct attatttgtc ggggtatttt tcttcttgtc atttattcca
    21601 ttgattcgca aatggaaaca tatcccggct cctgaacgtg aagaaaaatt gaactcccga
    21661 gaattctgga tgtttgtcgg atcgctggtt ttattgtttg gcggattaca tgtcatcttc
    21721 tttacaagca ttccggtttt caatactttg attacgcatg taaatgatat tttaggtacg
    21781 tctatccgca atgatcttgg tacacctgac gacccgattc acttttattc aggagtacaa
    21841 atttgggttg gtgtattgat tgccatgctg agtgcattcg cgcaatatct cagatacaaa
    21901 aactctgatg gtgcaggatt tgtcaaacgt atttcctatt cattcatttt cgccatagcc
    21961 gcaactgtta tcagctctat catgctggat aaactgtggc atataacatc tgaaaggggt
    22021 gtatttcatc tcatcacttc ttattcaata ttgctgtttg ccgcatggtt tagtgtgatt
    22081 gctaatgcgc agtattttat tcaggtgctt aaaggaaaga taagagtagg agggggttcc
    22141 gttgcgcata tcggtttcgg aattttatta ctcggcatac tgatctctaa tgcaggtcaa
    22201 cgtgtcattt cactaaatac cggcgatgtc cgtttcgatg ctcaagggat ggatgatacc
    22261 tttcagcgtg aaaatgtgag gctgataaag aatgtgcctt atcccatcac agattataat
    22321 gtaacctatt taggagatac actgattgca ggagcaactt attatgattt gttgttcaca
    22381 aaaacagatc cggagacagg tgaattgatg caacagttta cagttcatcc ttatttgttg
    22441 atggatacta aaatgcagca gttagcacca aatccggata cgaaacatta tctcacatac
    22501 gatgtgttta cacatgttac ctcacttccg ggtgaaggtt caaaaaatgc tccgcctaca
    22561 acaactttag atacaatagg agttggcgat acacttcatt tcgataccgg atatatgatt
    22621 ttgaaggatt tgaagaatac cagtttttca gcagattcaa atgaggtgaa ggcatttttt
    22681 gatgttcatg ccggtattgc ttctgaatct atagaagtgc catttatcgt atcagggcgc
    22741 accatgcaag tattgcaagg aacagcaaat gtatcagttg ccaatgtgca ggtttcgttc
    22801 attaccattt taccagatca atcgaaaaag tttgtgttta aggttgatga cagttcactc
    22861 aatactgatt ggattgttat aaaatccatc atattcccaa tgataaatct tgtttggatt
    22921 ggtgtgattg taatggcatt cggatttttt atgagtatgc tggctcgact tcgcgaacgc
    22981 aaagaaaaat ctgttgtcaa tggatgaaat taaatccatc ttgctcgtag gcaccggaaa
    23041 cgcagcctgg catcttggtt taaatttcaa gctgaatgga gtgttaattg ccggtattgt
    23101 agcgcgtgat gttgaaaaag gaattgcttt atctgctgca ttaggtgatg ttccggtata
    23161 tggtttggaa gacaagctgc cggaagttga tgcaacaata ttattagtgc gtgatgatgc
    23221 agttgtagaa gtggcgggca aagtggcaca agaaggaaaa ttgctggtgc attgttccgg
    23281 ttcaataaaa gcggaggcta ttaaatccgg tgaacatgca tttgcggtag cctggccttt
    23341 gcaaacattg caaaaagatc atccgcgccc attgtttaat accgttgttg cggtaaccgg
    23401 tagcgatgcg gaaactgaac aactggtagc aggactattc ggtaagatca gcaatcgcgt
    23461 gcatgtgatg agtgaagagc agcgatctgt gatgcatatg tgtgccgtgt ggatcaataa
    23521 ttacacgaac cacatgtatt acattggtca ttctttgttg aaggaaaaag gactggaatt
    23581 ttcgatgttt tttcctatca tcgaagagca tgtgcaattg ttacatgagc aagatcctga
    23641 aaaactgcaa acagggcctg caaaacgcgg cgatatggga actttatcac gacatatggc
    23701 tttgttaaaa gatcatcccg aatggcagga tctttacaac caattgtcgg gtagtatttt
    23761 aaaaacgtat ttaaagaaat aattttgtgt tgaatttcag gctatggtaa cgatcaaatt
    23821 tacttttgag gatggcagcg aagcgaaaga agtgcaagtg gaagagggtt attccgtatt
    23881 ggaagcagct ttgctgaaca atattaaatt aaatcacaat tgtggtggtg tttgcgcatg
    23941 cagcacttgc catgtttata ttgaaaaagg catggaggcg cttcctgaaa ctacggaaaa
    24001 ggaggaagac ttcatagaca gagctcgcga cccgcgatta aattcgcgac tcagttgcca
    24061 atgcgatctg atggaagatg gtacctttat tgaagtgcta gttccggatc aaagtactat
    24121 tattgcatca taatatattt attcagctat atgtctcaat ttgaagaacc acctatttat
    24181 tggaatgatc atgaagatat cgcactgaaa ttgtacgagc gatttggtga tgatttcaat
    24241 gaatcaaaaa tttatagaat tcgattcacc gaactcatcg agtgggtgct ggaaattcct
    24301 gattttaaag gaacacgtga ggaatgtaat gaaggccacc tcgaaatggt tcaaagtgca
    24361 tgggtatatg aatggcgcga taatcaataa cacttgtata ccattttatt gtctacattc
    24421 gttatcctgt aaacctcatt gcctgtgaaa gattggatac gcatcattcg tcctgttaat
    24481 ttattattgc tcgcagctac tctgtatgcc atcgacgggt ttattctgca accgaatttc
    24541 aatacctatg gaattcgttt ttcattgaat gaatttcagt atttcttgct ggttatctct
    24601 attgtcttga tttgtgcagg aggctatatc ctcaatgatt atttcgatgt gcagattgat
    24661 gaagttaaca aaccctcgaa agtgatgata gggaagggga tagctccaaa ttcagccttt
    24721 tatgtatata tgttgctcag ctttggtggt ttggccattg gaacatacct cagtttggca
    24781 gttggcaact ggaagcttgt tacaatttat gtgatggcaa ttgcattact gtatttttat
    24841 tctgcaacct ttaagcgata tcctttaatc ggaaatatga tggttgcttt attggccggc
    24901 ataagcattt tgatgacatt tatttatgaa cccaacctgt atcaacttgc aagacccgga
    24961 gattatttca tcgccggaat ttgcactaaa tttataatcg ctcttggtgt gatcgctttc
    25021 acatcaacca tggttaggga aattgtgaaa gaccttgaag atatggaagg cgatgatcgc
    25081 tacggcgcta aaacaatgcc cattgcctgg ggaataaata tcgctaaggt tataactgtg
    25141 gtttttgtat tattgacgct gcttggtatc ggttatatat ttacaaacat tattgcagcg
    25201 gaaggtatca tatatggcgc ctatattggc gtgatgcttg gcttctgggt tgcagtgagt
    25261 gttcgtcttt tcttgtcaag aaccgctaag caatatcaca cactaagcac catgataaaa
    25321 attggtatgc ttatcggttt attgatcatg cctttgtatt cgatcgtaga attttaatat
    25381 gaagatcatt cttgcatcag gctctccgcg ccgcagtcat attctctcaa ctgcgggatt
    25441 taattttaca gtcatggtgc cggatgtgga agaaacgttt cctgattcca tgccggtaat
    25501 ggatgtgccc ctgttcatag cggacaaaaa attgcaggcc gtgcttacgg atacatccgt
    25561 tgacaatatt gttatcaccg cagatacagt tgtaatatta caggacagga ttatcggtaa
    25621 accgattgat gaagctgatg cttttcagat tctaaaatcg ttaagcggga atatgcatct
    25681 tgttgtttcc ggtgtttgca tcgcatatca aggaaagcaa tacgatattg aagtgactac
    25741 caaagtgtat tttgatcatc ttaccgatga agagatttcg aaatacctcc aagaatgtaa
    25801 gccatttgat aaggcaggtg catacgccat tcaagaatgg ataggcatga ataagattac
    25861 gcgcattgaa ggcgactatt acaatgtagt aggatttccc atgagcaaga tttatccggt
    25921 gttgactgca ttaatggaat gatcagattg tattttcctt gatcacagca atcatcttgt
    25981 ccacttcttc aaaggtatta taatagtgtg gtgaaatgcg aacggcgcca cttactcctt
    26041 tttgatcgaa atccagtaac gcaaatttcc gtgcggcagc gcccgcattt attccgtttc
    26101 tgttcaatat ttctttgaaa tacatcgggt cactgcgatc cgaggtgagc gaaataatgg
    26161 cactcaaacg cgcacctcta tcctgcaaat gaatatgcgg aatggctgaa agctgttgtc
    26221 ttgcata
//
LOCUS       DAOQOU010000029        25377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_915_length_25377_cov_5.717242, whole genome shotgun sequence.
ACCESSION   DAOQOU010000029 DAOQOU010000000
VERSION     DAOQOU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25377
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_915_length_25377_cov_5.717242"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLM90_04300"
     CDS             <1..436
                     /locus_tag="PLM90_04300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ88620.1"
                     /translation="SFGSILEERLLPLVFQGYGRIVYVGIYLLGMIVSDVPSYFKHRT
                     DATYRGLGASGAVAAVVFASILISPFDGEIGFFLIPGLHVKPFVFGIIYLIYSAYMGR
                     RGGDNVNHDAHFYGALFGLIFPGLIKPELFISLYHQILQQWT"
     gene            427..1452
                     /locus_tag="PLM90_04305"
     CDS             427..1452
                     /locus_tag="PLM90_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HMZ88621.1"
                     /translation="MDIIDLRSDTVTKPSVEMRAAMASAQVGDDVFMEDPSILELEQK
                     ICAMFGTEMALFTPTATMSNQIALKILTHPLDEIICDRTAHIYSYEVGGYAFHSGCSI
                     RYVEGDRGVFSATDVIKQINPDDVHKPITRVVSIENTVNRGGGRIYPIETIAEIAAVC
                     REKGLSFHLDGSRLFNALVASGADAKQFGELFDTITLCFSKGLGCPAGSILTGKAIHL
                     QKARRVRKVMGGGMRQAGVLAAAGLYALEHNISRLAEDHQRAAETGNALNKLSYVESV
                     LPVETNIVIFRLRENISDEKFIQYLNHLGIKAFAIGDNWVRFVFHLDIISGMEEKIAS
                     ALINFNA"
     gene            complement(1449..1937)
                     /locus_tag="PLM90_04310"
     CDS             complement(1449..1937)
                     /locus_tag="PLM90_04310"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HMZ88622.1"
                     /translation="MIAQLLNLAEDHLHGISHNIPELNDLDSNQVTSVTSETVVNTIV
                     QQARKGNVDALREMLSGSETAKDHEVIRQLENPVINQLQSKLNISDQTAKQLAVIALP
                     VVMNLLNGKVKHAQTGGFDINDAIRGLNGNSGMLKGIFNLFGGNKSSTKTINSILQNL
                     IG"
     gene            complement(2000..3664)
                     /locus_tag="PLM90_04315"
     CDS             complement(2000..3664)
                     /locus_tag="PLM90_04315"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ88623.1"
                     /translation="MKSTITAIFLIIFGLQSQAQLFDPEHAYTRADTLRGSLRAERTC
                     YDVLTYDLAVKVDIANKAIEGSNTINFKAVSDFQRLQIDLFEELSITKIIFEGKDIQF
                     KREFAAVWVDFPKVIKGGTQGEFTVYYSGHPRIAKMAPWDGGISWNTDEKGNPWVGVS
                     CQGLGASVWWPNKDHLSDEPESMRIAITAPDTLMAVANGNLISTSIPEKGWKTWHWAV
                     TYPINNYNVTMNIGKYVHFEDTYTNVDGSTYSLDYYVLEYNVDKAKKQFEQVKPMLKA
                     YEQYLGAYPFPRDGYALVETPYVGMEHQSAIAYGNGYQTGYSGFDYSGIGLDFDYIII
                     HETAHEWWGNNVSMKDLADMWIHEGFGTYSEAIYVEALYGNATAMNYINAMKQKVRND
                     EPIIGDYNVNNEGSGDMYHKGAIFLNSLRTLTNNDQLWWSIIKGIQKDFAQQTVTTEQ
                     IERYISRKAGKDFSKEFDQYLRHKDLPKFEYKLTPEAGAVKLEYRWSADVPEFDMPLR
                     YMGADGAWKWLKPTTDWQETSLDLPNIKAFKLDTTHFYFEERMVKK"
     gene            3874..4281
                     /locus_tag="PLM90_04320"
     CDS             3874..4281
                     /locus_tag="PLM90_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88624.1"
                     /translation="MFNIRNSSLALLLPIAIGFLASGCTSLPGDGGTSTIHGKIYVER
                     YNEFGTLYQDYYGPDERVYIIYGDNIGYDDEVKTSFDGTYKFPFLRKGTYTIYAYSDC
                     LTCDGNTEARSVQVEITENNQDIEVEDIVIEAR"
     gene            4336..5034
                     /locus_tag="PLM90_04325"
     CDS             4336..5034
                     /locus_tag="PLM90_04325"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HMZ88625.1"
                     /translation="MDKVELMNRKDTKYFFDRKKLIEILDLLQHDYRVLEIGGHRIFN
                     YRNIYFDTPEFECYHMHHNKRVNRYKIRIRQYVESNLNFLEIKFKINTGRTIKSRIKI
                     AEFSPILSEEAKRFISEKTNFDPEQLRPTLENDFRRFTLVSNSMRERATIDTDLRFKT
                     PNGQMQLSNVCIAELKLDSSSSGSPFKLAMRDNFCPEARISKYSVGAAFMYSDLKQNN
                     FKFKKLQINRIENV"
     gene            5027..5707
                     /locus_tag="PLM90_04330"
     CDS             5027..5707
                     /locus_tag="PLM90_04330"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HMZ88626.1"
                     /translation="MFSTLIQAAEEQTKFLDLKFFDDDVYDLILRSFFNFVIVFILVK
                     FTYRSDRKNKNYAFSFYIFSMLVFFLCYLLSGIKLEMGFAFGLFAIFSILRYRTISIP
                     MKEMTYLFLVIAVAVINALTTKKVSFVELLFTNVSLIAATYFLERLWYREGLSEQIVE
                     YEKIENIKPERRAEMLEDLRIRTGLDIRSFEILNTDFLRDMAKVRIFFKPTPHFGEED
                     MEHDDDDD"
     gene            5730..6332
                     /locus_tag="PLM90_04335"
     CDS             5730..6332
                     /locus_tag="PLM90_04335"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HMZ88627.1"
                     /translation="MLTLSVCAKAQYTDGGVWLYSGINHEVVKDLKLEFGEELRYNIS
                     TDNPYQYNTHFSAAYKLNKHWKIGADYRYSVREGLNTNRYGAAITYKQSIHDLDISLR
                     SRFLYSVVLDGAEGTAWRNKLGCDYKINKDFQVGVSGELFYAMSNELDQFDNYRLQAG
                     LTYSPNKYHSLDVGYIYDHEFNVNNPGSMHVANIGYVYSF"
     gene            6298..6573
                     /locus_tag="PLM90_04340"
     CDS             6298..6573
                     /locus_tag="PLM90_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88628.1"
                     /translation="MSRILGTCIVFSICLCLSKNTNAQIITCDSLSTDTLSVIETPNS
                     LMINSTILFQKLAVPELSLPAYTQGVFCNFEDRINKKTPLRIDFSVK"
     gene            6624..7535
                     /gene="hemF"
                     /locus_tag="PLM90_04345"
                     /pseudo
     CDS             6624..7535
                     /gene="hemF"
                     /locus_tag="PLM90_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013390.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
     gene            complement(7532..7948)
                     /locus_tag="PLM90_04350"
     CDS             complement(7532..7948)
                     /locus_tag="PLM90_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88629.1"
                     /translation="MFAMLLAACVKPPVYPIEPYIEVVSINQDNFDELDEDSLSITLY
                     FEDGDGDIGSEDEVNMFWEDSRAPGFQIPFKIPYVQSQGNTKAISGTITTYYPISFCI
                     DDSDNIDTFYYKIFIVDRAGHISNTDSTQLIFLHCL"
     gene            8021..9004
                     /locus_tag="PLM90_04355"
     CDS             8021..9004
                     /locus_tag="PLM90_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HMZ88630.1"
                     /translation="MQDIARFTESIFEVTADTFAGKALAIFNYQAKENAVYRDFLQYL
                     DISPSSVKSISDIPFLPISAFKHHKVVCGGLETDTYFESSGTTDSQRSRHYIVDRMTY
                     ENSYLSSFRQVYGDPINYRILALLPSYLERSNASLVDMCRGLIERSGHPESGFYLHDL
                     QSLSQVLSNDTGIKTILIGVSFALLDLAEQFPQSLTNTIIMETGGMKGRRSEITREAL
                     HDVLRNAFNVDVVHSEYGMTELFSQAYSTGYGIFQCPPWMRIIIRDSEDPLQLLETGK
                     TGGVNIIDLANLHSCSFIATDDLGSIQQDNRFSIAGRFDNSDIRGCNLMTL"
     gene            complement(9018..9563)
                     /locus_tag="PLM90_04360"
     CDS             complement(9018..9563)
                     /locus_tag="PLM90_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ88631.1"
                     /translation="MQKTPNAALSRLSVEQYKNADKHPFVVVLDNVRSQNNVGSVFRT
                     ADAFLLRGICLCGITATPPHRDIEKTALGATESVYWEYFDHTVDAIKKLKELGYGIVA
                     VEQTSGSVALQDFDPSSFEKIAFVFGHEVMGVSEDALALCDASIEIPQFGTKHSLNIA
                     VSAGIVLWDIVMKHKGQSASK"
     gene            complement(9614..10126)
                     /locus_tag="PLM90_04365"
     CDS             complement(9614..10126)
                     /locus_tag="PLM90_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ88632.1"
                     /translation="MKDKQFKVFLVDDDTKHLIMLKNHLEKRSDYDLKVNIFSNGENC
                     LDKMHEQPHVVILDYYLDGIKTDAANGLEILKKIRLQYPDTYVIMMSGQDDLKVAVAT
                     IDNGAYDYIIKGESAYVRAQMIIDHIIENINTEEWRKNVKVRENILVTGFILLGLVLI
                     SIALYDMGVF"
     gene            complement(10116..12134)
                     /locus_tag="PLM90_04370"
     CDS             complement(10116..12134)
                     /locus_tag="PLM90_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ88633.1"
                     /translation="MSEQQFPELLRELLSKYYLHSKSIERELYKSELKFRSLLEQLTD
                     AIYFTSVDGKFIEFNRATVDLLGYSSDELRELNVEATYVNPEDRDKFKAIVTANGEVI
                     DYELKLKSKSGDIIDCLVTSTVRYDSKGVILGYQGIIRDITLRKKAESLQRAKDLAEQ
                     ANKFKAEFLANMSHEIRTPINAIIGMVHLLHQTPLSSKQQEYLQAIDSSADNLLQLIN
                     DILDFSKIEAGKLQLENKSFSIRKVIEQLVNTMRFKADEKAIELKLNIDENVPESVAG
                     DPLRLNQIMLNLISNAIKFTHAGNITVTVRLIDTINDKARMYFNVRDTGIGIAPDKLN
                     HIFESFTQANMDTTRIYGGTGLGLSIVKKLIDMLNGAIMVKSKPGNGSEFIFEIDFTI
                     TRDAIELDQASSNDSGIPEIGACRMLLADDYPINQLVTTELLKHTWREMIIDVANNGK
                     EVLELLSKNDYELILMDVQMPEMNGLDATREIRKLQGPKAQIPIVAFTAYATTGEVDK
                     CLEAGMDDYISKPVDPATLCRKIVEQLVKRNYQFPDPSAPKLAIEGVSPDLSYLDQMT
                     GEDSELKFKMINIMLEETPTELELLDSYCKAGNWDGVRAVAHKMKSSLQYMGLKETLE
                     LAKSIELAARDKVDVDQLPERINNVILTCKAALTSLSAELEKMQENEG"
     gene            12218..13189
                     /locus_tag="PLM90_04375"
     CDS             12218..13189
                     /locus_tag="PLM90_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ88634.1"
                     /translation="MHSIAELSTLIEEGIFNAGFPQQPTGLYDPARYILSVGGKRIRP
                     LLALMGAQVFADDVKPHLPVALAMEVFHNFTLVHDDIMDNAPLRRGKQTVHEKWDVHT
                     AILSGDAMMIQSYVLLAGGPSNALMPLIDMFNKTALAVCEGQQDDMDFEKSEEVEIDA
                     YLEMIAKKTSVLLGCSLYCGAISAGANEDQAALLYEAGLKLGISFQIQDDILDTYGDS
                     SFGKQTGGDIARNKKTILFLMARDLANETDCALLLRLYHDSASSESEKIAAVRNIFDK
                     YAVKEHAESLMARYYEESRAALSSLHSTKNGVDPLLKLFDQVFTRTT"
     gene            complement(13191..13820)
                     /locus_tag="PLM90_04380"
     CDS             complement(13191..13820)
                     /locus_tag="PLM90_04380"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HMZ88635.1"
                     /translation="MIREFAQKLQKSLEFGLPGAPAHSIMAPLTRNVEPVEIGKLPNA
                     KKAAVLILFTPLDDKPAITLIERPAYEGVHGGQMSFPGGRIEESDVDVRYTALRETNE
                     ELGIDTSNIVTIGKLSDVYIPPSNYYVYPYLGYLPYAPEYFPDAREVKSVVTMPVSIL
                     FDPLSKGKMLIERPGISFEAPCYVYQQFRIWGATAIMLSELEWLLTRQV"
     gene            13894..16905
                     /locus_tag="PLM90_04385"
     CDS             13894..16905
                     /locus_tag="PLM90_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ88636.1"
                     /translation="MKRRHTLLLLLLSPFAAFGQHHAKTGDLQFIENVGQWESPVEYV
                     AGVYGGELFLEKNAFTYHFSNAAELGHFKHDPNAGDPSSFVIKNHAFKVHFLHSDPLV
                     QLQGESIFDNYYNYFLGNDQSKWKGKVSAFGAVRYANLYPGIDMQVYSQSQSMKYDYI
                     IQPGISPAAIQIGYEGLDAIQLNDGNLELKTSINTVVEVKPLAYQIIQDKKVIVPCSF
                     VLNGNVVSYSFPEGYNTEYSLIIDPATLIFASYTGSTADNWGYTATYDNDGNLYGGGI
                     TFANGYPVTIGAYQETWTPGAGAYVADITVSKFSADGTSLIYSTYLGGSNEDLPYSLI
                     VNEANELIIYGATGSNDFPTTAGAYDASFNGGVLETVDYVLTFTSGSDAFVAKLSADG
                     ADLVGSTYIGGTANESLNTGVTSYNYGDHARGEVNLDGAGNIYIAGCTRSTNFPTTAG
                     VMQNSNGGALDGFVAKFNPALTSLSWSTYVGGNGDDGAYAIKYLSSGDVVIVGGTSSN
                     NLNTTPSALHASYQGGLTDGYVLVMTPDASSIAAMTYIGTSAYDQTYLLDVDELDNIY
                     ITGQTLGDYPIVGTVYSNPGSAQYISKLNTNLSTLLLSTLLGDGDAQVNISPTAFLVD
                     NCHNIFLTGWGGLVNSGFNPATGSMVSMPITADAFQSSTDGSDFYLIIFGNELETMAY
                     ATYFGGSISDEHVDGGTSRFDKQGIVYHAVCAGCGGNDDFPTTEGVVSNTNNAFNCNL
                     GVFKFSLGPPPTAAAFLATPDKGCVPLEVFFENESINADSYEWDFGDGTTGIATDESH
                     VYTEPGTYTIRLVASVDDECGINDTVYQTVEIYEIPIADFTSSPDIASVYYPVEFTDA
                     SIVAVEWDWSFGDGFTSSLQNPTHLYADRGTYTVCLVATNADGCSDTTCREITITALS
                     VLDVPNAFSPNGDGTNDVFLPLNYGLTDFLFRVYNRWGELIYETTDPTKGWNGIYNGV
                     EQEMDVYVYTVSGKGEDNVFYSKQGNFTLVR"
     gene            complement(16907..18139)
                     /locus_tag="PLM90_04390"
     CDS             complement(16907..18139)
                     /locus_tag="PLM90_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88637.1"
                     /translation="MKINNLLNAFILCFSILTTVFVLDGCEPPGCTREYNFTGEMPVY
                     MTYEELRASVYLDAPKPIDSSNAFCISDPYLFVSDFEKGVHVYDCTIPENPQDIGFIH
                     VPGVTQLAAVGSRLYVNSYVDLVVLDISDIHAIHETGRVENTLTYRTYGSIFADPSKG
                     VIIGFEERDTVIVEECGYHSSGIGMVYYDEPYLYMPYASGKQSGYLSYYKANGDSLIC
                     FDEGTIRIFDVNNSDNPTVSFTTEVGGTYTVEPIGEYFLIGNSIYTSPASNQLNYLSY
                     LSEADYCSKIVVNQNYCYAANRTTDECYSYGTNLVAINIDYVTSPNYLDYYTDVVPND
                     VFTLNDWVFVIHDHSELRMYHIEADGTLTLFDSIPVENAEYVKASENTVIVDTNPGYK
                     IYTYNGTSGLEYASYIPE"
     gene            18287..20086
                     /locus_tag="PLM90_04395"
     CDS             18287..20086
                     /locus_tag="PLM90_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88638.1"
                     /translation="MRILTLATLSFLFIQTNKAFSQACATCSIDYTYTSPGVYPDTLP
                     PATAGEFYEADITFVMQDDTTVDVVGTLDFLNYYIMEPVGMPYGMHVSTDLGDLPVNY
                     DPTVSLYGCARVCGTPLVAGWYEVTVPLVATLEFPGGDQAASYSIYLEVLPAAPTGGG
                     IIPTASFGCEPLNVDFETGVHSMGADGFSYTWDFGNGASSTSEFPPSQSYVAVGGVPT
                     DYVIMHTVSIDTIGYTLDYVTALSSGCDDCTIFGCTGLIESEKPDLYIIIADLGVNTE
                     PGFANTSPPVTFSLGNTLDPLHTYTMQMKDDDGGLAGSDDNCGSFTFDGNDVGISTLS
                     AGSHQVEVSISHPVITYTFYDTITVYPEVTVPVLDILGETTFCSTDSAVLSTTEVAGL
                     TYQWYKDGDPIPGGDATSLVVYTSGSYFLSATGTGGCYANSDSVVITVFEQPTAPNII
                     VIGNTLTTSSTWDVQWYYNGDPIPGATSTSYTPAIEGLYQVAAVNGPCMAYSVEVDFV
                     FQSVNEVFMPSLQITPNPNEGQFICDFSVTELQDIQIRVTNMLGEVVYSNELHNIIGN
                     NQFAIDLTPADTGIYLLDIQSSKGHIIRKIVVQ"
     gene            20100..21482
                     /locus_tag="PLM90_04400"
     CDS             20100..21482
                     /locus_tag="PLM90_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ88639.1"
                     /translation="MTYSEIIHLQRKYYAEGHTLSYAFRRQQLEKFLELVIAHEEAIL
                     DALQKDLHKSSFEGWGVEVGVIITETKYFIRHLKHWMKPKRVKTPLLHFRSRSYIKPV
                     PHGNTLIIAPWNYPFLLAMRPLIGAIAAGNTAIIKPSEHAIHTAKLMEAMINDHFDPA
                     FIRVLLLDASGSAALSAEKFDFIYYTGGANIGRSIYTSAAKNLTPVVLELGGKNPCII
                     DETADIDITASRITWGKFSNCGQTCVAPDYLLVHTSVKDKLVTAIIKNIESFFQNELI
                     NTEDYGRIINDFHVERISRLISNMKIIYGGDVLPNQRYISPTLIEIDDLQSPIMQEEI
                     FGPVLPVLTYNDFSEIKQIIAHNPDPLVLYIFSKNKQHIGICSNEIGSGDMVVNEVIL
                     HFGHLHLPIGGKGSSGFGKTQGKYTFNAFSHQKSLMHRSYFPDLTVRYPPYNSRKSGF
                     IRWVFKYFFS"
     gene            21576..22769
                     /locus_tag="PLM90_04405"
     CDS             21576..22769
                     /locus_tag="PLM90_04405"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ88640.1"
                     /translation="MHDLAARIANLNESATIKMAQLTRELKGQGRDIVDLSLGEPDFE
                     TPLHIREAAKKAIDDGWTHYTPVAGYLEVRQAVSEKFLRENGLHYSPEQIVISTGAKQ
                     SIINAVLCLVNPGDEVILPTPFWVSYSAMVKLADGVIKEIPTSIASDFKITPDQLEAA
                     ISSKTKLIIFSSPCNPTGSVYTKEELFDLAKVLEKHPHVYVISDEIYEHINFTDKHAS
                     MAQFDFMYDRVITVNGLSKAFAMTGWRLGFIGAPVWIAKACDKMQGQYTSATCSITQR
                     ATIAALTGDIEPMLVMKDAFLRRRDLVYGLLKAIPGLKVNNPQGAFYFFPDVSNYFGK
                     QYQGQIVNNADDMALYLLNEGNVSVVTGSAFGDQNCIRLSYATADEKLIEAMKRMADA
                     LSKLQ"
     gene            22772..23767
                     /locus_tag="PLM90_04410"
     CDS             22772..23767
                     /locus_tag="PLM90_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HMZ88641.1"
                     /translation="MATSKPDIKKLFRQFNDMRVMIVGDVMLDKYIYGQVDRISPEAP
                     VPIVDVRSATARPGGAANVAVNIAALGATPYLFSVTGKDHDADLLCELMEEQHIDPQY
                     LLHAADRITTSKMRVVAKQHQMLRIDHEMQDELQEETAITFLQNVVGFIKKKKPHVVI
                     LEDYDKGVLSASLITEIISKCHTLHIPVVVDPKKKHFFHYKGCTLFKPNLRELRDSLD
                     GNPSTATISDMKLAVAALQTLMPHHISMVTLGEYGVFIQEGKKSRIIPAHHRNIADVS
                     GAGDTVISVAALCLAAGADIFTTAAISNLAGGLVCEHAGVVPVDKQQLLKEALAN"
     gene            complement(23779..25050)
                     /locus_tag="PLM90_04415"
     CDS             complement(23779..25050)
                     /locus_tag="PLM90_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88642.1"
                     /translation="MLGIFFKTIAETMGLVFQELRNNKLRTSLSLLGVSIGIFCIIAV
                     LSAVDSLKRNMESGLNKLGTDMIYITRWPWQFSNEEYTWWEYVKRPTMKYDEFQQLSD
                     KLTLAKAVAIETWLSPQEVKFMDHTLQNASISGVTSQYDEVYTMNFMSGRYFTDGESV
                     GGSEVAILGYEVAQELFPGGLQPVGKYFWVRANGSQFKLRVIGVLEKEGESIIDISND
                     NSIIIPFNYIRRIVDMNSPMIDPMIEVRPKEGVTADEIKDEIYPIMRNIRNLRPAEQN
                     DFAVNEITLAADALDNLFAALNMVGMIIGGFSILVGGFGIANIMFVSVKERTNVIGIK
                     KSLGAKNYLILTEFLIEAIVLSLIGCLFGLLLVWVLMLVAENLSDFGFILSWTNIAIG
                     AIISISLGVVAGIVPAITASRMNPVEAIRSR"
     gene            25159..>25377
                     /locus_tag="PLM90_04420"
     CDS             25159..>25377
                     /locus_tag="PLM90_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HMZ88643.1"
                     /translation="MKLSKFNFELPKDIIAQHPTAERDQSKMMVVHRKTGKIEHKKFR
                     DILGYFDENDVMVLNNTKVFPARLYGRKE"
BASE COUNT         7349 a         5191 c         5236 g         7601 t
ORIGIN      
        1 gtctttcggt agtattctgg aagagcgttt attaccgcta gtttttcaag gatacggacg
       61 aatagtttat gttggtatat atttattggg tatgatagta tctgatgtgc cttcctattt
      121 caaacatcgc accgatgcaa catatcgcgg acttggggct tcaggcgctg tggcggcagt
      181 tgtttttgca agtattttga tcagtccttt tgatggtgag atcggctttt ttctaattcc
      241 cgggttgcat gttaaacctt ttgtttttgg aattatatac ttgatctact ctgcgtacat
      301 gggtcgacgt ggtggtgata atgtaaatca tgatgcgcat ttttatggtg ccttatttgg
      361 attgatattc cctggtctaa taaaaccgga actttttatt tctttatatc atcaaatctt
      421 acaacaatgg acataattga tttacgcagc gacacggtaa cgaaaccaag tgttgaaatg
      481 cgcgctgcta tggcatctgc acaagttggc gatgatgtat ttatggaaga tccaagtatt
      541 ctggagctgg aacaaaagat ctgtgcgatg ttcggaacag aaatggcgtt gttcacacca
      601 acagctacta tgtcgaatca aatagcttta aaaatattaa ctcatccact ggatgaaatt
      661 atttgtgata gaacagcaca tatatatagc tatgaagttg gcggctatgc ttttcattca
      721 ggatgttcca ttcgctatgt agaaggcgac cgcggtgttt ttagtgcaac agatgtcatc
      781 aaacaaatta atcctgatga tgtgcataag ccgataacgc gagtggtgag tattgaaaat
      841 acagtaaatc gcggcggtgg tcgcatctac cctattgaaa caatcgctga aattgctgcg
      901 gtatgtcgag aaaagggatt gtcatttcat ttagatggtt cgcgattatt taatgcgctt
      961 gttgcaagtg gtgctgatgc aaagcaattt ggagaattat tcgataccat tacattgtgt
     1021 ttcagcaaag ggttgggatg tcctgcaggg tctattttaa cgggcaaggc aatacatctg
     1081 cagaaagcac gcagggtgag aaaagtgatg ggaggcggga tgcgtcaggc cggagtttta
     1141 gctgctgccg gattgtatgc gttagagcac aatatcagtc gacttgcaga agatcatcaa
     1201 cgtgcggccg aaacaggaaa tgcattaaac aaactgagtt atgtggaatc ggttttaccg
     1261 gtagaaacga atattgttat cttcagacta cgtgaaaata tatcagatga aaaattcatc
     1321 caatacttaa atcatctagg aattaaagca tttgccatag gagacaattg ggtgcgtttt
     1381 gttttccacc ttgatataat atcaggcatg gaagaaaaaa tagcatcagc cttaataaat
     1441 ttcaatgctt aaccaatcag gttttgcaga atactgttaa tggtttttgt gctgcttttg
     1501 tttccaccaa acaaattaaa aatacctttc aacataccac tattgccatt taatccgcgt
     1561 atggcatcgt taatgtcaaa tccaccggtt tgtgcatgct taactttacc attgagcaag
     1621 ttcataacca caggtaaggc gattacggct aattgttttg cagtttgatc agaaatattc
     1681 aacttactct gcaattggtt aatcacagga ttctccagtt ggcgaataac ttcatgatct
     1741 tttgcagttt ccgaacctga cagcatttct cttagtgcat caacatttcc ttttctggct
     1801 tgctgcacga tggtatttac aacggtttcg gaggtaacac ttgttacctg attactatcc
     1861 aggtcgttca attccggaat gttgtgggag atcccatgca ggtgatcttc ggctaggttc
     1921 agtaattggg cgatcatgat gttcgggttt aaggtgtatg taaatctaaa aaaacgcctt
     1981 ggttattcca aattttacat tatttcttca ccattcgttc ctcaaagtaa aaatgtgtgg
     2041 tatcgagctt aaatgcctta atatttggca ggtcaagcga ggtttcctgc cagtcggtgg
     2101 taggtttcag ccatttccat gctccatcgg cacccatata tcgcaacggc atatcgaatt
     2161 caggcacgtc ggccgaccag cggtattcca atttcaccgc accggcctcg ggcgtcagtt
     2221 tgtattcaaa tttcggcagg tctttatgtc ttaaatattg gtcaaattct tttgagaaat
     2281 ccttccctgc ttttctcgaa atataccttt caatttgctc ggtagtaaca gtttgttggg
     2341 caaaatcctt ttggataccc ttgatgatac tccaccacag ttggtcgttg ttcgtaagcg
     2401 tacgcaacga gttcaggaat atggcacctt tgtgatacat gtcgccactg ccttcgttat
     2461 tgacattgta atccccgatg atcggttcat catttcttac tttttgcttc atggcattga
     2521 tataattcat ggcggtagca tttccatata atgcctccac atagattgcc tcggaatacg
     2581 tgccgaagcc ttcatgtatc cacatatcag ccagatcttt catgctcaca ttgttccccc
     2641 accattcatg cgcagtttca tgaataatga tgtaatcaaa atccaggcct atccctgagt
     2701 aatcgaagcc tgaatagcct gtttgatatc cgtttccata cgcgatggca ctttggtgtt
     2761 ccatacctac atatggcgtt tcaaccaggg catacccgtc tcgaggaaaa ggataggcgc
     2821 ccagatattg ctcatatgct ttcagcattg gctttacttg ctcaaattgc ttttttgcct
     2881 tgtcaacatt gtattccagc acataataat caagcgaata tgtagatcca tcaacattgg
     2941 tgtatgtatc ttcaaaatga acatacttgc cgatattcat ggtaacatta taattattga
     3001 ttggataggt aaccgcccaa tgccatgttt tccatccttt ttccggtatt gaagttgaaa
     3061 tcaggttacc attagcaact gccatcagtg tatcaggtgc agtgattgca atgcgcatac
     3121 tttcaggttc atcactcaaa tgatctttat tcggccacca gacactggct ccgagccctt
     3181 gacatgaaac accaacccat ggattcccct tttcgtcggt attccagctt attccaccat
     3241 cccatggagc catttttgca attctggggt gaccgctgta ataaacagtg aattctcctt
     3301 gagtacctcc cttgattacc tttggaaagt cgacccaaac agctgcaaat tcacgtttaa
     3361 actgaatatc cttaccctca aagatgattt tagtgatgct gagttcttca aacaagtcga
     3421 tttgcagtcg ctgaaagtcg ctcactgcct taaagttgat ggtatttgag ccttcaatag
     3481 ccttattagc aatgtccact tttacggcca gatcgtaggt aagcacatcg tagcaagttc
     3541 tttcggcacg caggcttcca cgcaaggtat cagccctggt ataagcgtgt tcaggatcga
     3601 atagctgggc ttgggattgg agtccaaaaa tgatcagaaa tattgctgta atggtagatt
     3661 tcatgtgctg tacttgtttc gaatcacaat aataacaggt ttttaaggtc aaatctcgtg
     3721 cccttaacgc gaattaacag acatgcaata tgaaacttag tcaaaaaggc gctcaggcac
     3781 catctaaata aggtggttac aaacagataa caatagattt acaatttcct gttaccttta
     3841 ccttttaacg ttgtctttac caattgctaa cacgtgttta acattagaaa ctcctcctta
     3901 gcgctcttat tgcctattgc gataggtttt ctggcatcag gctgcacttc gctcccgggt
     3961 gatggtggca cttcaaccat acatggaaag atctatgtag agcgttacaa tgaatttggt
     4021 accttgtacc aagattatta cggacctgac gaacgtgtct acatcattta tggagataat
     4081 atcggctatg atgatgaagt aaaaaccagc tttgatggca cttataaatt tccgttttta
     4141 aggaagggca cgtataccat ctatgcctat agcgattgcc tcacatgcga tgggaataca
     4201 gaagcgcgct ctgtgcaagt agagatcaca gaaaacaacc aggatattga agttgaagat
     4261 attgtaattg aagcccgtta attgcaccgc atagattcga tattacatca aattcacccc
     4321 atcacacttg gtgagatgga caaggtggag ttaatgaatc gaaaggatac taagtatttt
     4381 ttcgacagga agaagctcat agagatatta gatctgctgc agcacgatta tcgtgtatta
     4441 gaaattggcg gacatcgcat tttcaattat cgcaatattt atttcgacac tcctgaattc
     4501 gagtgctatc acatgcatca taacaagcgt gtgaacagat ataaaattcg cattcgtcaa
     4561 tatgttgaaa gcaatctgaa tttcctggag atcaaattca agattaatac cggtcggacg
     4621 attaaatcgc gcatcaagat cgctgaattt tcgccaatac tgagtgagga agctaaacga
     4681 tttatttcag agaagacaaa tttcgatccg gagcaattgc gcccgacctt agaaaatgat
     4741 tttcgtcgtt tcacactggt aagtaattca atgcgtgaaa gggccactat cgataccgac
     4801 cttcggttca aaacgccaaa cggtcaaatg caattgagca atgtgtgtat agctgaatta
     4861 aagctcgatt ccagttcatc aggttcgcca tttaaattag ccatgcgcga taatttttgt
     4921 ccggaggcca gaatcagcaa atacagtgta ggcgctgctt ttatgtatag cgacctgaag
     4981 cagaataatt tcaaattcaa gaaactccaa attaaccgta tagaaaatgt ttagcacttt
     5041 aatccaggca gccgaggaac aaaccaagtt cctagacctg aaattctttg atgatgatgt
     5101 ttacgatctg atactaaggt cgttcttcaa ttttgtgatt gtttttattt tagtaaaatt
     5161 cacctatcgc agcgatcgta agaataagaa ctatgcgttt tcattctaca tcttcagcat
     5221 gttggttttt ttcctatgtt atctgttgtc gggcattaaa cttgaaatgg gttttgcatt
     5281 tggattattt gcaattttct caatcctgag gtaccgcacc atcagcattc cgatgaagga
     5341 aatgacttac ctctttttgg taattgctgt tgctgttatt aatgcactaa caactaagaa
     5401 ggtatccttc gtggaattat tattcaccaa cgtttcgctg attgcagcta cttatttcct
     5461 cgaacgcttg tggtatcgtg aaggcttgag tgaacagatt gttgaatacg aaaagatcga
     5521 aaatatcaag cctgaacgca gagcagaaat gcttgaagac ctgcgcatca gaaccggtct
     5581 ggatatacgt tcattcgaaa tattaaatac agactttttg cgcgacatgg caaaggttcg
     5641 catattcttt aagcctacac ctcatttcgg cgaagaagac atggagcatg atgatgatga
     5701 cgattaatag agtcatatta agcatttgct tgctaacact ttcggtatgt gcaaaggctc
     5761 aatataccga tggtggcgtt tggctttata gtggcatcaa tcacgaagtg gtaaaagatt
     5821 taaaattaga attcggtgaa gagctgcgat ataatatcag cactgacaac ccataccaat
     5881 acaatactca cttttcggct gcttataaac taaataaaca ttggaaaatt ggtgccgact
     5941 atcgctattc agtccgtgaa ggattaaaca ccaatcgata tggcgctgca ataacctaca
     6001 agcagagtat acatgatttg gatatcagtt tgcgcagccg gtttttgtat agtgtagtgc
     6061 ttgatggggc tgaaggtaca gcctggcgta ataaacttgg atgtgattat aagattaata
     6121 aagattttca ggttggcgtt tccggggagt tattttacgc tatgagcaat gaattggatc
     6181 aatttgataa ttatcgatta caagccggcc tcacctactc tccgaacaaa tatcatagtt
     6241 tggatgttgg atatatttat gatcatgaat tcaatgttaa caatccgggg tcaatgcatg
     6301 tcgcgaatat tgggtacgtg tatagttttt agcatttgtc tgtgtttatc gaaaaacact
     6361 aatgcacaga ttataacctg cgatagcctg tcaactgata cactcagtgt gattgaaacg
     6421 cctaattcat tgatgatcaa cagcactatt ttgtttcaaa agcttgccgt tccggaatta
     6481 tcgctgcctg cttacaccca aggtgtcttc tgcaattttg aagacaggat caacaaaaaa
     6541 acacctttaa gaattgattt cagcgtaaag tgaaggtgct gaaaatcacc accgatgtat
     6601 atcaataata ataaatttat tccatgcgaa atgtagatcg atttgcaata agtgattggt
     6661 tcaaaggctt gcaagatcat atttgcgatg aaattgccag caccgatggc aagggtgtat
     6721 tcgagcagga gctttggcag cgcgaaggcg gcggtggtgg tagaacccgg atcatacaaa
     6781 atgccaatat tcttgagaaa ggcggcgtaa attattctga agtgcatggt gtcttacctt
     6841 cccgtatcgc tgatgctatg caggtttcag gtcatgattt tttcgcaaca ggcgtatcca
     6901 tcgtaatgca tccttttagt cccatggtgc ctatcattca catgaatgtg cggtattttg
     6961 aagtgagcag tggtgagaaa tggttcggag gaggtattga tcttacacct atttatattg
     7021 acaagaccca agctgcggtt tttcatcgca aattgaaaga ggtatgcgat cgtcatcatg
     7081 aaaattatta tgcggaattc aaacaatagg ctgatgatta tttctatatc aaacatagaa
     7141 aggaaacgcg tgggataggt ggtattttct ttgatagatt acaggcaaca gaatacattt
     7201 cactttcgga cagatttgaa tttgtgaaac aagtcgggat gttgttttca gaaatatata
     7261 cgcgtctgat gcaggaaaat aaagatattc cgtatggcaa accggaaaag ctttttcaag
     7321 ctttgcgacg cggcagatat gtggaattca atcttgttta tgacaagggc actaaattcg
     7381 gacttgacac tgatggcaga gcagaatcta tattaatgag tcttcctcct gaagccggtt
     7441 ggccatataa ctataaacca attgccggta gtcgggaaga acaaaccata gatctgcttc
     7501 gcaagggtgt ggaatgggct tcggcggata tttagaggca gtgtagaaaa attaattgtg
     7561 ttgaatcagt gttactgata tgccctgccc tatccacaat gaatattttg taataaaagg
     7621 tatctatatt atctgaatcg tcgatacaaa acgaaattgg ataatatgtg gtaattgtgc
     7681 ctgaaattgc tttcgtatta ccctggcttt gcacataagg tattttgaac ggtatttgaa
     7741 agccgggagc cctgctgtct tcccaaaaca tatttacctc gtcttcactg ccaatatcac
     7801 cgtctccatc ttcaaaatat agtgtaatgc tcaggctgtc ctcatccagc tcatcgaaat
     7861 tatcctgatt aatacttacc acttcgatgt aaggttcaat aggataaacg ggaggcttta
     7921 cacatgctgc gaggagcatt gcgaacatta taattaggaa gctgtatttc ttcaccataa
     7981 ttttaccatt caaaggtata tcctttataa ctcagaaaca atgcaagata ttgcgcggtt
     8041 cacagaaagc attttcgaag ttacggctga tacatttgcc ggcaaagcgc ttgccatttt
     8101 caactatcag gcgaaggaaa atgctgtgta ccgtgatttt ttacaatatc tcgacatttc
     8161 tccttcctca gtgaagtcga tttccgacat ccctttttta cctatttctg ccttcaaaca
     8221 ccataaagta gtttgcggag gccttgaaac ggatacctat ttcgaaagta gtggaaccac
     8281 tgatagtcag cgcagcaggc attatatcgt tgacaggatg acgtatgaaa acagttattt
     8341 gagcagtttc aggcaagtat atggtgaccc gattaactac cgcattttgg cactgctccc
     8401 ctcctacctg gagcgatcaa atgcctcgtt ggtggatatg tgtcgggggt tgatcgaacg
     8461 gtcgggccat cctgagagtg gcttttattt acatgatctg caaagcttat cacaggtttt
     8521 gagcaatgat acaggaataa agaccatact gattggtgtt tcctttgcct tgcttgatct
     8581 ggcggagcaa tttcctcaat ccctcacaaa taccattatt atggaaaccg gaggcatgaa
     8641 aggaagaaga agtgagatta cacgagaggc cttacatgat gttttgagaa atgcttttaa
     8701 tgtcgatgtg gtccattctg aatacggcat gacagaatta ttttcacaag cctattcaac
     8761 aggatacgga attttccaat gtccgccctg gatgcgcata atcatacgcg acagtgagga
     8821 tccgttacaa ctgttggaaa ccggaaaaac aggtggcgtg aatatcatcg acctggcaaa
     8881 tcttcattcc tgcagtttta ttgccaccga tgacctcggg tccatacaac aagataatag
     8941 attctctatt gccgggagat ttgacaacag cgatattcgg ggttgtaatt taatgacctt
     9001 ataagactaa ggttacatta ttttgaggcc gactgccctt tgtgtttcat tacgatatcc
     9061 cataagacaa tacctgcact tacagcaata ttaagtgaat gtttagttcc gaattgagga
     9121 atctcgatgg atgcatcaca caatgccaat gcatcttcac ttacgcccat cacttcatgt
     9181 ccaaaaacga atgcaatttt ttcgaatgag gaaggatcaa aatcctgtaa ggctacactg
     9241 ccggaagtct gttctacagc tacgatgcca tatcccagtt ccttcagttt tttgatagca
     9301 tcaaccgtgt gatcaaaata ttcccaataa acgctttcag tagcacccaa ggctgttttt
     9361 tcaatatcgc gatgcggagg agtggcagta ataccgcaca ggcaaattcc cctaagcaaa
     9421 aatgcgtctg ccgtgcgaaa tactgatccc acattatttt gacttcggac attgtccaaa
     9481 accacaacaa aggggtgttt atcggcattt ttatattgtt ctaccgataa tctggataaa
     9541 gctgcgtttg gcgtcttttg catacgaggg ctgcaaagta acggaattgg gctaacttat
     9601 ggtatttgaa ttgtcagaat acgcccatat catacaaagc gatgctgatc aacaccaatc
     9661 cgagcaggat gaaaccggta acgaggatgt tttcacgcac ttttacattt ttgcgccact
     9721 cctcggtatt aatattttcg ataatatgat ctatgatcat ttgagcacgc acataggcac
     9781 tttcgccttt gataatataa tcatatgcac cattgtcaat tgttgcaact gctactttca
     9841 aatcgtcctg cccgctcatc atgattacat aagtatcggg atattgcagt cggatttttt
     9901 tcagaatttc cagaccattg gcagcatccg tttttatgcc atcgagataa taatccagaa
     9961 taaccacatg cggttgttca tgcattttat cgaggcagtt ttcaccattt gaaaatatat
    10021 tcaccttcag gtcataatca gacctttttt cgagatggtt tttcaacata atcaggtgct
    10081 tggtatcgtc atctaccaga aacaccttaa attgcttatc cttcattttc ttgcattttt
    10141 tcgagttcag ccgaaagact ggtcaatgcg gctttacatg ttaaaattac attattaatt
    10201 cgttccggca actgatctac atcaacctta tcgcgggcgg caagttcgat ggatttggcc
    10261 agctccaggg tttccttgag tcccatatat tggagcgaag acttcatttt atgcgctaca
    10321 gccctgacac catcccagtt tccggcttta caataagaat ccagcagctc gagttctgtg
    10381 ggtgtctctt ctagcatgat gttgatcatc ttgaatttaa gttcggagtc ctcccctgtc
    10441 atttgatcca ggtaactcaa atcaggcgaa acaccttcaa tggccagttt aggtgctgat
    10501 ggatcgggaa attgataatt gcgttttacc aattgttcca caatctttct gcaaagcgtg
    10561 gccggatcca caggtttcga aatataatca tccattcctg cctccaggca tttatcaact
    10621 tcccctgttg tagcataggc agtaaatgcc acaattggaa tttgtgcctt tggtccctgt
    10681 aattttcgta tttctcgtgt agcgtcgagg ccattcattt caggcatctg cacatccatc
    10741 aaaatgagtt catagtcatt tttcgaaagc aattccaata cttctttgcc attgttcgcc
    10801 acatcgatga tcatttcacg ccaggtatgc ttcaataatt ctgttgtaac caattgattg
    10861 attggataat catctgctaa tagcattcgg caggcaccaa tttcaggaat gccactatcg
    10921 tttgaagatg cttgatctaa ttcaattgca tcgcgtgtaa tggtgaagtc aatttcaaaa
    10981 ataaattcac tgccattgcc gggtttgctt ttaaccatga tagcgccatt aagcatatcg
    11041 atgagtttct ttacgataga taatcccaat ccggtgcctc cataaatgcg ggtagtatcc
    11101 atgttggcct gtgtaaaaga ttcgaagata tgattcagct tgtcaggggc aattccaatt
    11161 cccgtatcac gcacattaaa atacatgcga gctttatcat tgattgtatc aattaatcga
    11221 acggtcacag tgatatttcc agcatgggta aatttaatgg cattgctgat gagattgagc
    11281 atgatctgat ttaaacgcaa agggtcgccg gcaacagatt caggaacgtt ttcatctata
    11341 tttaatttca gttcgatagc cttttcatca gccttgaatc gcatggtgtt tactaattgt
    11401 tcgataacct tgcgaatgct gaatgattta ttctcaagct gtaatttccc tgcttcaatt
    11461 ttagagaaat ctaaaatatc attaatgagt tgcaataagt tatctgctga agaatcaatt
    11521 gcctgcaaat attcttgttg ttttgaagaa agcggcgttt gatgcaataa atgtaccata
    11581 ccgatgattg cgttgatagg cgttcgaatc tcatggctca tgtttgccaa gaattccgcc
    11641 ttgaatttat ttgcctgttc cgctaggtct ttggcacgtt gcagtgattc cgcttttttt
    11701 cgcaatgtaa tatcgcggat aataccctga tagcctaaaa taaccccttt actatcatag
    11761 cgtacggtag atgttaccag acaatcgatg atatcgccac ttttactttt taacttcaat
    11821 tcataatcta tcacttcgcc attggcggta actatcgctt tgaatttgtc gcgatcttca
    11881 gggttaacat aggttgcttc aacatttaat tcacgcaatt catctgaact gtatccgagc
    11941 aagtcgaccg ttgcccggtt gaactcaata aatttcccat caacgctcgt aaaatatatg
    12001 gcatccgtta attgctcgag gagagatctg aatttgagtt cacttttgta caattccctt
    12061 tctatcgatt tactatgtaa atagtatttc gatagaagtt cacgtaagag ttcgggaaat
    12121 tgttgctcag acatccgcta caaattaacg caattagctg cactttaaag caattttgga
    12181 ggtagctgag ccgctttgat tgccttatct ttgcggcatg cacagcattg ctgaattgtc
    12241 tacgctaatt gaagagggta tttttaatgc cggattccct caacagccca ccggtttgta
    12301 tgatcctgca agatatatat tgtctgtagg cggcaagcgt atcaggccgc ttctggcact
    12361 aatgggggca caggttttcg cagatgatgt aaaaccgcat cttccggtgg cattggcaat
    12421 ggaagtattc cacaatttca ccctagtgca cgacgatatt atggacaatg cacctcttcg
    12481 tcggggaaag caaacagtgc atgagaaatg ggatgtccat actgctatct tatcaggtga
    12541 tgccatgatg atacaatcat acgtattgtt ggcaggtggc ccgtcaaatg cgctaatgcc
    12601 attgatcgat atgtttaaca aaactgcact ggcggtttgt gaagggcagc aagatgatat
    12661 ggattttgaa aaaagtgaag aggtagaaat cgacgcctat cttgaaatga tagcaaaaaa
    12721 gacttctgta ttattgggtt gcagccttta ttgcggtgcc atttctgccg gcgcaaatga
    12781 ggatcaggct gcattgcttt atgaagcagg gttaaagctg ggcatcagtt ttcagattca
    12841 ggatgatatt ttagacactt atggcgacag tagttttggc aaacaaacag gtggcgatat
    12901 tgcccgcaat aagaaaacca ttttgttttt aatggcgcgt gatctggcca atgaaactga
    12961 ctgcgcattg ctgttgcgtt tatatcatga ttctgctagt tcggaatccg aaaaaatagc
    13021 tgctgttcgc aacattttcg ataagtatgc cgtgaaagaa catgccgaat ccttgatggc
    13081 aaggtattat gaggaatcga gggctgcatt gtcaagcctt cattcgacaa agaatggagt
    13141 tgatcctctt ttgaagttat ttgatcaggt tttcacgcgc accacctgaa ttaaacctgt
    13201 cttgtcagca accattccaa ttcactgagc atgatagctg ttgcgcccca aatgcgaaat
    13261 tgctgataca cataacatgg tgcttcaaag gaaatacctg gacgttcgat aagcattttc
    13321 ccttttgata gcgggtcaaa caatatcgaa accggcatgg ttactactga tttcacctct
    13381 ctggcatcag gaaagtattc gggagcataa ggcagataac ccaggtaggg ataaacatag
    13441 taattactgg gaggtatata aacatcacta agtttaccta tagtaacaat attgcttgta
    13501 tcgataccca attcttcatt cgtttccctc aaagcagtat aacgaacatc cacgtcgctt
    13561 tcttcaattc gccctccagg aaagctcatt tgaccgccat gaacgccttc atacgccggc
    13621 cgttcgatca atgtgatggc gggcttgtca tctaaggggg taaaaagtat taaaacggct
    13681 gcttttttcg cattcggtaa tttgcctatc tctacaggtt cgacattcct ggtaagcggc
    13741 gccatgatac tgtgtgccgg ggcgccgggc aggccgaatt caagactctt ttgaagtttt
    13801 tgcgcgaatt cccgtatcat atttaggtca tttgacatgc aacctcacaa agatgattta
    13861 tttaccgtaa attagtagcg tgaaatattg tgcatgaaaa gaagacatac tctcctccta
    13921 ctactgctat cgccatttgc ggcttttggt caacatcatg caaagaccgg cgatttgcaa
    13981 tttatagaga acgtcggcca atgggaatcg cctgttgaat acgttgccgg tgtgtatggc
    14041 ggtgaacttt ttcttgaaaa aaacgccttt acttaccatt tttcaaacgc tgctgaatta
    14101 ggacatttta aacacgaccc aaatgcaggc gacccttcat catttgttat taaaaatcat
    14161 gctttcaaag tacacttttt acattccgat cctttagtgc aattgcaggg cgaatccatt
    14221 ttcgataatt attataatta ttttcttggc aatgaccaat caaaatggaa aggaaaagta
    14281 tctgcatttg gtgcagtgag atatgcaaat ttatatccgg gaatcgatat gcaggtatat
    14341 agtcaaagcc agtccatgaa atatgattat atcatacagc cgggcatctc acctgctgca
    14401 attcaaattg gttacgaagg attagatgct attcaattaa atgatggcaa tcttgaatta
    14461 aaaacaagca ttaataccgt tgtagaagta aaaccgcttg cctatcaaat cattcaagat
    14521 aaaaaagtta ttgtgccttg ctcatttgta ttaaatggta acgttgtcag ctactcattc
    14581 cctgaaggat ataatactga atattcttta atcatagatc ccgctacctt gatttttgct
    14641 tcatatacag gaagcactgc cgataactgg ggatatacag ccacatatga taacgatgga
    14701 aacctgtatg gcggaggaat tacctttgcc aatggatatc cggttactat aggagcctat
    14761 caagaaacat ggacccccgg agccggcgcg tatgttgcag acatcactgt ttcaaaattt
    14821 tctgctgacg gcacttcatt aatatattct acctatctcg gtggttctaa tgaagatcta
    14881 ccctattcat tgattgtaaa tgaagctaat gaactaatca tttatggtgc aacaggatct
    14941 aatgatttcc caaccaccgc aggcgcctat gatgcttcgt tcaatggtgg cgtgcttgaa
    15001 acagtagatt atgtgcttac atttacgtca ggcagcgatg catttgttgc taaattaagt
    15061 gccgatggcg ctgatttagt tggttcgacc tacatcggag gcactgctaa tgagtcgctg
    15121 aatacaggcg taacaagtta caattatggt gaccatgcac gtggtgaagt aaatcttgat
    15181 ggggccggaa acatctatat tgcaggatgc accagatcaa ctaactttcc gactacagca
    15241 ggtgtaatgc aaaattccaa cggcggtgca ttagatggat ttgttgcaaa atttaatccc
    15301 gcattgactt cattatcatg gtcgacatat gttggcggta atggcgatga tggtgcttat
    15361 gccataaaat acttaagttc cggcgatgta gtaattgttg gcggcacaag cagcaataat
    15421 ttaaatacta caccttcggc cttgcatgca tcctatcaag gaggcttaac cgatggctat
    15481 gtgttggtga tgacacctga cgcctcgagc atcgcagcca tgacctatat cggaaccagc
    15541 gcctacgatc aaacgtattt acttgatgtt gatgaactgg ataatatcta catcactgga
    15601 caaacacttg gcgattatcc tattgtagga actgtgtaca gcaatcccgg aagtgcacaa
    15661 tatatttcga aattaaatac aaatctcagc accttattat tgagtacctt gttgggtgac
    15721 ggagatgctc aggtaaatat ttcacctact gcatttttgg ttgacaattg ccataatatt
    15781 tttctgaccg gttggggagg attggtaaac tccggattta atcctgcaac tggaagtatg
    15841 gtgagcatgc ctattacagc agatgcattt cagtcatcaa ccgacggaag cgatttttat
    15901 ttaattattt tcggaaatga actggaaacg atggcatatg ctacctattt tggcggcagt
    15961 atttctgatg aacacgtaga tggcggaaca agtaggttcg ataagcaagg aattgtttat
    16021 catgctgttt gtgcaggatg tggtgggaat gatgattttc ctacaactga aggcgttgtt
    16081 tcaaatacca ataatgcatt taattgcaat ttaggagtat tcaaattctc tttaggtccg
    16141 ccgccaactg cagctgcatt tttagcaacg cctgacaagg gctgcgttcc cctggaagtg
    16201 ttttttgaaa acgaaagtat aaatgccgat agttatgaat gggattttgg cgatggtact
    16261 acggggattg caacagacga atcgcatgtt tatacggaac cgggaacata cactattaga
    16321 cttgttgctt cagtagatga tgagtgtggt ataaacgata ccgtttatca aacagtagaa
    16381 atttatgaaa tccctattgc agattttaca tcgagtccgg atatcgcatc tgtttactac
    16441 ccggtagaat ttacagatgc cagcatagta gctgttgaat gggattggag ctttggtgat
    16501 ggatttacct cctctttaca gaacccgact cacctgtatg ccgaccgcgg aacatacact
    16561 gtatgccttg tagcaacaaa tgccgacgga tgttcagata caacctgtcg cgagattaca
    16621 ataactgcct tgagtgttct ggatgttcca aatgcatttt caccaaatgg agatggcacg
    16681 aatgatgttt tcctcccatt aaattatgga cttaccgatt ttctatttcg cgtttacaat
    16741 cgctggggag aattaattta cgaaaccaca gatcctacaa aaggatggaa tggcatctac
    16801 aatggcgtag aacaggaaat ggatgtttat gtgtataccg tttcaggtaa aggcgaggac
    16861 aacgtctttt actccaagca agggaatttt acacttgtaa gataaattat tccggaatat
    16921 aggatgcata ttccaatcct gaagtgccgt tgtaggtata aattttgtaa cccgggtttg
    16981 tatctacaat aacggtgttt tctgatgctt ttacatattc tgcattttca acaggaattg
    17041 aatcaaataa ggtgagggtt ccatcagctt ctatatggta catccttaat tcgctgtgat
    17101 catggatcac gaaaacccaa tcgtttaatg taaaaacatc gttaggtaca acatcggtat
    17161 aataatctaa ataatttggc gatgttacgt aatcgatatt tattgccact aaattagtcc
    17221 cataactata gcattcatcc gtagtcctgt ttgccgcata acaataattt tggttaacga
    17281 ctattttgct gcaataatca gcttcagaaa gatacgaaag gtagtttaac tgattacttg
    17341 cgggagatgt ataaatagaa tttcctataa gaaaatattc tccaataggt tcaacagtat
    17401 aagtgccacc cacttccgtt gtaaacgata ccgttgggtt atcggaatta ttgacatcaa
    17461 aaatgcgaat ggtaccttca tcaaagcaaa tcaacgaatc gccgtttgct ttatagtacg
    17521 agagatatcc gctttgttta ccgcttgcat atggcatata taaatatggt tcatcatagt
    17581 ataccatgcc aattccggag ctatgataac cgcattcctc aacaataacg gtatctcttt
    17641 cttcaaaccc gataattact ccttttgatg gatctgcaaa tattgaacca tacgttctat
    17701 acgtaagtgt attttccacc ctacccgttt catgaatagc atgtatatct gaaatatcta
    17761 atactacaag atcgacataa ctgtttacgt agagtctgct tcccacagct gccaactgcg
    17821 ttacaccggg aacatgaata aatccgatgt cttgtggatt ctcaggaata gtacaatcat
    17881 acacatgcac acccttttcg aaatcgctga caaacagata tggatcactg atacaaaatg
    17941 catttgaact atcaataggc ttaggtgcat ctaaataaac agaagccctt aattcctcat
    18001 aagtcatata aacaggcatt tcacctgtga agttgtattc tctggtacag ccgggaggct
    18061 cgcatccatc taacacaaaa acagttgtta aaatggaaaa gcacaatata aaggcattga
    18121 ggagattatt aattttcata accggatttt aacaaaaata ccaaatacca tgaatatcat
    18181 gaatgacgta actcgtctaa agtatttccc tgagccggtc ctacttcaag cccacatcat
    18241 ttcacgcaaa ccaaaattcc actaacttca acccaaaatt gcatacatgc gcattcttac
    18301 ccttgccaca ctgtcatttc tttttattca gactaataag gccttcagtc aagcttgcgc
    18361 cacctgtagt atagactaca cctatacttc accgggggta tatcccgaca cattgccacc
    18421 cgcaactgcc ggcgaatttt atgaagcaga tatcaccttc gtgatgcagg atgacactac
    18481 ggtagacgtg gtcggcaccc tggattttct gaattactac attatggaac cggtgggaat
    18541 gccttatggg atgcatgttt caaccgattt aggcgatctc cctgttaatt acgaccctac
    18601 cgtatcactt tatggatgcg ccagagtttg tggcacgcct cttgtagcag gatggtatga
    18661 agttaccgtt ccactggttg ccacgcttga atttccggga ggcgatcagg cagcttcata
    18721 tagcatctat ctggaagtac ttcccgctgc gccaacgggt ggaggcatta taccaacagc
    18781 ttcatttggc tgtgaaccac tgaatgtcga ttttgaaacc ggcgtgcata gtatgggtgc
    18841 cgatggattc agttatacct gggatttcgg caatggcgca tccagcacct cagaattccc
    18901 gccttctcaa agttatgtag ccgttggagg agttcccact gattatgtga tcatgcatac
    18961 ggtttctatt gatacaatag gatataccct agattatgtg accgcacttt ccagtggatg
    19021 tgatgattgc acaatctttg gatgcacggg actgattgaa tccgagaaac ctgacttata
    19081 catcattatt gccgatcttg gcgtaaatac cgaaccgggt ttcgccaaca cttcaccgcc
    19141 ggttaccttc agcctcggaa atacactcga cccactgcac acctatacca tgcaaatgaa
    19201 ggatgatgat ggcggtttgg caggtagtga cgacaattgc ggctcattta cattcgatgg
    19261 aaatgacgta ggcataagca cattgagcgc aggaagtcat caggtggaag taagcatctc
    19321 ccaccccgtg attacttata cattttacga taccattacc gtataccctg aggttacagt
    19381 tcctgtactt gatatccttg gtgaaacaac attttgctct actgatagtg cagtactttc
    19441 aaccacggaa gttgcaggtt taacctacca atggtataag gatggtgatc ctataccggg
    19501 aggagacgca acttcacttg tggtatatac ctccggaagc tatttcctga gtgctacagg
    19561 aacaggcggt tgctatgcca attctgattc agtggtaatc accgtgtttg aacaaccaac
    19621 tgccccaaac atcatagtca tcggaaatac acttacaacg tcctctactt gggacgttca
    19681 gtggtattac aatggcgatc caattccggg cgcaacatcc accagctata ctcctgccat
    19741 tgaaggtttg tatcaggtag ctgctgtaaa tggcccatgt atggcatatt ctgttgaagt
    19801 ggattttgtg tttcaatctg ttaatgaagt atttatgcca agtttacaga tcacaccaaa
    19861 tccaaacgaa ggacagttca tttgcgactt cagtgtaact gaattacaag atattcaaat
    19921 tcgggttacc aatatgttgg gtgaagtggt ttattccaat gaattgcata atataatcgg
    19981 caataatcaa ttcgctatag acctcactcc tgcagataca ggaatttatt tactcgacat
    20041 acaaagtagc aagggacata tcatacgcaa gattgttgtt caataaacga ggtatttcta
    20101 tgacctattc tgaaataatt catctgcagc gcaaatatta tgcagagggt catactttgt
    20161 catacgcatt tcgaaggcaa caactcgaaa agtttcttga attagttatt gcgcatgaag
    20221 aggcaatcct ggatgcattg caaaaagacc tgcataaatc gtcattcgaa ggctggggcg
    20281 ttgaagtagg tgtaattatc actgaaacaa aatatttcat ccgacatctt aaacattgga
    20341 tgaagccaaa gcgtgtaaaa acgccattat tgcatttcag atcccgtagt tatatcaagc
    20401 cggttccgca tggtaatacc ttaatcatcg caccttggaa ttatcccttt ctgttggcca
    20461 tgcgtccact tattggtgct attgctgcag gaaatacagc tatcatcaaa ccatcggagc
    20521 acgccataca cacagcaaag ctgatggagg ctatgataaa tgatcatttc gatccggcat
    20581 tcatccgcgt attacttctt gatgcatccg gctcagcagc cctgagtgcc gagaaattcg
    20641 acttcatata ttatacagga ggcgcaaata tcggcagatc catttacacc tctgctgcaa
    20701 aaaatctcac cccggttgta ttagagcttg gtggcaaaaa tccttgcatc attgatgaaa
    20761 ctgctgatat tgatattaca gcctcgcgaa taacatgggg taaattcagc aattgcggac
    20821 aaacctgcgt tgctccggat tatttgttgg tgcatacatc ggttaaggat aaacttgtca
    20881 cggcgataat aaaaaatatt gagtcattct ttcagaatga attgatcaac actgaggatt
    20941 acggtcgtat tataaatgat tttcatgttg aacggatcag cagactcata agtaacatga
    21001 aaataattta tggcggagat gtattgccta atcaacgata tatttcacct acgcttattg
    21061 agatcgatga cttgcaatcg cccatcatgc aggaagaaat ttttggtcct gtattgcccg
    21121 tgctcactta caatgatttt tccgagatca aacagatcat agcacacaat ccggatccac
    21181 tggtattgta tattttttct aaaaacaaac aacatattgg tatttgctca aatgaaatag
    21241 gcagtggtga tatggttgtg aatgaagtga ttttgcactt cggacatttg catttaccta
    21301 ttggaggaaa gggaagcagt ggctttggaa aaacacaggg aaaatacacg tttaatgcat
    21361 tcagtcatca gaagagcctt atgcatcgta gttatttccc tgacttaaca gttcgatatc
    21421 cgccatataa cagtcgcaaa tccggcttta tcagatgggt ttttaagtat ttcttcagtt
    21481 agtgtccaaa acacgtggtt tcccacattg cagaaaagcc tgatttggag gtgaggccac
    21541 caatttttcc taatttcgca ccttaaacca gttgcatgca cgatttagcc gctagaatcg
    21601 ccaatctcaa cgaatctgct actatcaaaa tggcacaact cacacgcgag ttaaaaggtc
    21661 aggggcgtga tatagttgat ttgagcttag gcgaaccgga ttttgagacg ccattgcata
    21721 ttcgcgaagc tgcgaaaaaa gcaattgatg atggatggac acactatact cccgtagcag
    21781 gatatcttga agtgcgtcag gcagtttctg aaaaatttct ccgtgaaaat ggattgcatt
    21841 atagccctga acaaattgtt atttcaaccg gtgctaagca atcgattatt aatgctgttt
    21901 tatgcctggt gaatcccggc gatgaagtga tattacctac cccattttgg gttagttatt
    21961 ctgcaatggt aaaacttgct gatggtgtta taaaagaaat tccaacttct attgcaagcg
    22021 actttaaaat tactccggat caactggaag cagcaatatc ctccaaaaca aaattgatta
    22081 ttttttcttc gccatgtaat ccaaccggtt ctgtttatac gaaggaggag ctattcgatc
    22141 ttgccaaagt tttagaaaaa catccgcatg tttatgtgat cagtgatgag atatatgaac
    22201 atatcaactt caccgataaa catgcaagca tggcacagtt tgatttcatg tatgaccggg
    22261 taataacagt aaacggatta tctaaagcat ttgccatgac aggctggcgc ctcggattta
    22321 ttggtgcgcc tgtttggatt gctaaggcct gcgataaaat gcaaggtcaa tatacctcag
    22381 caacttgtag tatcacacag cgagccacaa ttgctgcact gaccggagat atagaaccga
    22441 tgttggtcat gaaagatgca tttttacgtc gccgcgatct tgtttatggt ttgttgaaag
    22501 caataccggg actaaaggtc aataatccgc aaggtgcatt ttattttttc cctgatgtga
    22561 gcaattattt cgggaaacaa tatcaggggc agattgtcaa taatgcggat gacatggcct
    22621 tatatttatt gaatgaaggt aatgtttctg tggtaacagg aagtgccttt ggcgatcaga
    22681 attgtattcg tttgagttat gctaccgcag atgaaaagct cattgaagca atgaagcgaa
    22741 tggctgatgc actttctaaa ttgcaataaa aatggcgaca tcaaaaccag atataaaaaa
    22801 actattccga cagttcaacg acatgcgtgt aatgattgtc ggtgatgtga tgttggataa
    22861 atatatctat ggtcaggttg atcgcatttc tccggaggca ccggttccaa ttgttgatgt
    22921 acgcagtgcc accgcaagac cgggaggagc ggctaatgtt gcggtgaata ttgccgcatt
    22981 aggtgccaca ccgtatttgt tttccgttac cggtaaagac catgatgctg acttgttatg
    23041 cgagttaatg gaagaacaac atatcgaccc acaatatctt ttacatgctg ctgatcgcat
    23101 cacaacatca aaaatgcgtg tggttgccaa gcaacatcaa atgctgcgaa tcgaccatga
    23161 aatgcaggat gaattgcaag aagaaaccgc catcacattc ctgcaaaatg ttgttggatt
    23221 catcaaaaag aaaaaaccac atgtagttat tttggaagat tatgataaag gtgttttatc
    23281 ggcatcattg attacagaaa taatcagcaa atgtcatact ttgcatattc ctgttgttgt
    23341 agatccaaag aaaaaacact tcttccatta taagggatgt acccttttta aaccaaatct
    23401 tcgagaactg cgcgacagcc tggatggaaa tccgtcaaca gcaaccatat ctgatatgaa
    23461 acttgcggtt gcagccttgc aaacgcttat gccgcatcat atttctatgg ttacattggg
    23521 ggaatatggt gtatttattc aggaagggaa aaaatctcgc atcatacctg cacaccatcg
    23581 caatattgcc gatgtgagtg gcgccggaga tactgtgata agtgttgctg cattatgtct
    23641 tgctgcaggt gctgatatat ttactaccgc agctatcagt aatcttgccg gcggattagt
    23701 atgtgaacat gcgggtgttg tgcctgtaga taagcaacaa ttgttgaagg aagctttagc
    23761 gaattaatta tactattgtc agcgagatct aatcgcttct acaggattca ttctacttgc
    23821 ggtaatggca ggaacaatac ctgcaactac acccaaactt atggagataa ttgctccaat
    23881 tgcgatgttt gtccaggata aaataaatcc gaagtcagat agattttctg caactaacat
    23941 aagcacccaa accaatagca atccgaacaa acagccaatc aaggaaagta caattgcttc
    24001 aattaggaac tcggtgagaa ttaaataatt ttttgcgccg agcgattttt ttatgccaat
    24061 tacattagtt ctttctttaa cgcttacgaa cataatattg gcaataccaa atccaccaac
    24121 cagaatgcta aatccaccta tgatcatgcc caccatattg agcgctgcaa acagattatc
    24181 aagtgcatcg gctgcaagtg taatttcgtt cactgcaaaa tcattttgtt cagcaggtcg
    24241 gagatttctg atattacgca tgatgggata gatctcatcc tttatttcat cggctgtaac
    24301 accttcttta ggacgcactt ctatcatagg atctatcata ggactattca tatccacaat
    24361 gcgacggata taattaaaag gaatgatgat agaattatcg ttagaaatat cgatgatgct
    24421 ctccccttct ttttctaata caccaatcac gcgcaattta aattgagagc cgtttgcgcg
    24481 cacccaaaaa tatttcccaa ccggttgaag tccaccagga aatagttctt gcgccacttc
    24541 ataacccaat atcgcaactt cagaaccacc aacagattca ccatctgtaa aatatcttcc
    24601 actcatgaaa ttcatggtgt acacttcatc atactggctg gttacaccgc tgatggaagc
    24661 attttgcaaa gtatgatcca tgaatttcac ctcttgtggc gacaaccatg tttcaatggc
    24721 aactgctttt gcaagtgtca atttatcgct gagctgctgg aattcatcat acttcatggt
    24781 cggacgtttc acatattccc accatgtata ttcttcatta ctgaactgcc agggccaacg
    24841 tgtgatataa atcatgtcgg tgcctaattt attcagcccg ctttccatat tgcgtttaag
    24901 cgagtctacg gcactcaaca cagctatgat acagaagata cctatagata caccaagcag
    24961 ggaaagtgat gtacgcagct tgttgtttcg aagttcctga aacaccaaac ccatcgtttc
    25021 tgcaatcgtt ttgaaaaaaa tccctagcat ctgccacaaa gttatcagat aaacgcggtg
    25081 aattagttat agaatggtta attttgcgcc cttgtcggcc taacttcagg ctcacaataa
    25141 ctaaaacccg taagttttat gaaactatcc aaattcaatt tcgagcttcc caaagacatt
    25201 attgcccaac atcctactgc cgagcgcgat caaagcaaga tgatggtggt acacaggaaa
    25261 acaggcaaga tcgaacacaa aaaattcaga gacattcttg gctatttcga tgaaaacgat
    25321 gtaatggtat tgaataatac caaggttttt ccggcaagat tgtatggccg caaagaa
//
LOCUS       DAOQOU010000030        24986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_936_length_24986_cov_5.753680, whole genome shotgun sequence.
ACCESSION   DAOQOU010000030 DAOQOU010000000
VERSION     DAOQOU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24986
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_936_length_24986_cov_5.753680"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLM90_04425"
     CDS             complement(<1..827)
                     /locus_tag="PLM90_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HMZ88644.1"
                     /translation="MIIGILGEGQSDQRVAIAAEHIPSLKALKAEKILIESNAGLAAG
                     SPDADYVAKGAEIASRAEVIAKSDMLIKIHPVGSDELAKMRGKVLIGGFNPLVNTSFV
                     SACLENGVSAFSLDVIPRTTRAQAMDILSSMATVAGYKAVLTAASHLPKFFPMFMTAA
                     GTITPAKVVILGAGVAGLQAISTAKRLGAVVEASDVRTAAREEVLSLGAKFIEVEGAT
                     EDKSAGGYAVEQSEDFKKRQQAKVQEHASKADVIICTAQIPGRKAPILLQKETVAQMR
                     "
     gene            953..1648
                     /locus_tag="PLM90_04430"
     CDS             953..1648
                     /locus_tag="PLM90_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HMZ88645.1"
                     /translation="MQTFKSDLSDKEFPIKEMVYATAVRPTIMTLIKADHPAFDESKG
                     LLSLSELSFYREKYIADYLKSEIADLSDLEHTVVKSLQDNSLLSDRADLEGDTNITTG
                     QRLADNVARFGGSWRFIILFGLFLAIWIMINALLLSNKGFDPYPFILLNLMLSCLAAI
                     QAPLIMMSQNRQEEKDRQRAKKDYMINLKSEMEIRLLHEKLDHLLMHQQQKLLEIQQF
                     QIEMMNDIYNRLK"
     gene            1677..1874
                     /locus_tag="PLM90_04435"
     CDS             1677..1874
                     /locus_tag="PLM90_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88646.1"
                     /translation="MPEDEFYEDSVQTMEDFIRYVHELRKDLKTNPEDFENNTLPLFK
                     ELISKIPLASFLCLRPLSFVT"
     gene            2182..2751
                     /locus_tag="PLM90_04440"
     CDS             2182..2751
                     /locus_tag="PLM90_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88647.1"
                     /translation="MKTFCTLICFLLFVLPAEAQQDSVFLKYPVDIDSLGKAIYSVDT
                     ILFRAYHDGDILIGTTVIPGTNNSMNLMNHGYRLHNIRLMPCDGGDVFEVVSRVNAVR
                     ETDSTLEVDVTITDNCCFNFLCDARIDEINVMHLFYTGYGMNCSCFCCFGLTYVFERW
                     NFTNDPLIDAVMIADDKKSLYNISRDAKD"
     gene            2738..3415
                     /locus_tag="PLM90_04445"
     CDS             2738..3415
                     /locus_tag="PLM90_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88648.1"
                     /translation="MRRIKLLLIAFVSIISLRAQPELEFCRVAPIVYFDSVDNKPMLN
                     LPVGGKWIDSLEFSLNGNYKNSFVSVDIGVQGSGAEILRESDSLWRISLFAYFDFSSD
                     SDTAQEIEVARLILTKNEAGYICKTDLSFRPDPVELKGEQLHNLFSSCANNNLYKLSA
                     FDIGESEWDTLSLLINDLFVLSLHGDALALKEFNDMLILFPAVKMYELHHYYMSARAV
                     LCLYGMK"
     gene            3460..4251
                     /locus_tag="PLM90_04450"
     CDS             3460..4251
                     /locus_tag="PLM90_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HMZ88649.1"
                     /translation="MFRKSIPLFIIVLFISSCTMIRNTTNTSPGQEPLFLSKWTLDNL
                     HGSTISIESQPYLLFTASETFRVSGFAGCNLITGGFTLSGVDSIDFGEIASTLMACPQ
                     YATEQDFLTALQEANRWMISGGQLFLLQGSDVVASFTGSTIDVAEEAGNMAVNGTWEL
                     SYISGPRIAFDALYPQQKPTLVISMPNTKATGNSGCNSFSVDVKIKDKTILFGPIAAT
                     KMYCEGSGESTYFDNLGKVDTWQMDDNNNLALMIGDVVMMRFIRK"
     gene            4308..6425
                     /locus_tag="PLM90_04455"
     CDS             4308..6425
                     /locus_tag="PLM90_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HMZ88650.1"
                     /translation="MKSILTLLCGVLLSTSLPAVTVTSPDRTLLLNVDLDKSGALHYS
                     LSRNGAVVIAPSLMGFSLKDLGELTADFEAVDTVYNSIDTYWEPVWGEVAKIHDNHNE
                     MTFTVRHKKTGIVMEVIFRMFNDGMGFRYQFPVQSYMKYFTVLNEKTQFCLNGDHQAW
                     WIPGDYDTNEYFYTESKLSDVHAITASTRNREIATRSPIGDNYVQSPLMMKSSDGLYI
                     NIFEAALVNYPAMHLEVDREKHILTSRLATDAVGNCAYLSTPCHTPWRTVIVSDKATD
                     ILGSHIILNLNEPCAIANTDWIKPMKFVGIWWEMHVGTASWNYSDVNNINLETTDWTK
                     LPPNGHHGATTERTKYYIDFAAANGFDGVLVEGWNVGWEDWFGNWKENVFDFVTPYPD
                     YNVQELSAYAKSKGVSLIMHHETSASVTNYERRLQQAMQFMVSNGYPAVKTGYVGRII
                     PRGEYHDGQWMVNHYVYVAQEMAKHHLMVDAHEPVRPTGLQRTYPNWLACEAARGNEF
                     NAWSQGNPPEHETILPFTRLMGGPMDYTPGIFEVQMSTYGQQTGNQVHTTLAKQLALY
                     VTMYSPLQMAADIPENYLKYADAFQFIKDVAVDWQDTKYLEAEPGDYLTVARKEKGGR
                     NWFVGAITDDKARRTSIKLDFLDDKQVYIATIYGDAEGADWKTNPKAYMITQQKVTNM
                     STIKLELAPGGGAAVSIMPIQEN"
     gene            complement(6532..7746)
                     /locus_tag="PLM90_04460"
     CDS             complement(6532..7746)
                     /locus_tag="PLM90_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HMZ88651.1"
                     /translation="MRPTLSHFRSILYLFTASMFFASCAVDPPMVNDDSFRGEIISST
                     LLHDWSITQTDSALSDYDPSLLTYPNDYNIKIYRVVYRTIDAAGNETQASGAVVIPYG
                     VANGAAQCLYMHGTVVERFEVPSYESDELVLGMLYGANGFVTSLPDYLGMGDSPGRHP
                     YCHSATEASAGIDLLRATKTLCANEGVSLNNQLFIFGYSQGGHAAMAATKAIQEDYAD
                     EFTITASAPMSGPYDLSGAQTDYVLQDAPYGAPFYLPYLMFGYNDIYKMYDNYADYLA
                     SPYDTLLPSLFDGTHSGWEIDAVMPVIPKNIIKPEVLEDFINNPNNPFRVALRENDLT
                     DWTPQIPMIMYYCGGDELVNYQNSIIAQDAFVANGSTTTSLYQPSATASHTDCAEPCF
                     IFANIWFSSLRQ"
     gene            complement(7803..9167)
                     /gene="hemN"
                     /locus_tag="PLM90_04465"
     CDS             complement(7803..9167)
                     /gene="hemN"
                     /locus_tag="PLM90_04465"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HMZ88652.1"
                     /translation="MSIDQFRLLSKYDVAVPRYTSYPTVPSWDFQTIQGDNWKSAVIR
                     SFKQENQELCIYIHLPFCEELCTYCACNKRITKNHAVEIPYLESVLAEWEMYRALLAD
                     TPVIREIHLGGGTPTFFSPEHLKQLINGITKHAIVANDHAFSVEVHPNYTNEQHLVAL
                     QEVGFNRISLGVQDFDPVVQFIINRPQTFEQTQHVIKTARALGFTGVNIDLIYGLPKQ
                     TNDSIKLTMEKVKELMPDRIAFYSYAHVPWKSKGQRRYTDEDVPVAEEKLQMYQTGSA
                     YLHQLGFSHIGMDHFALPTDALMLAYQVGKLHRNFMGYTTTNHKLVIGLGASAISDTW
                     DAFAQNEKGVEAYQDAIASGRLPIINGHNLNETDQLIRKHILDLMCRDTTTLVEEQLP
                     AGTFDAIMERLSGFVEDGLISIEGGKISILPSGRLFIRNICSAIDLHLHSGSRQGNTF
                     SKAI"
     gene            9445..12054
                     /gene="clpB"
                     /locus_tag="PLM90_04470"
     CDS             9445..12054
                     /gene="clpB"
                     /locus_tag="PLM90_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HMZ88653.1"
                     /translation="MNLQQFTIKAQEVIQQAQQMAMQMDHQYIMPEHILKALLSDDEV
                     AGYLLKKLQANPVYIQQKLDEQLAAVAKVASGQGGQYISREANLVIAKSQTFLKEFND
                     EFVSVEHLLLGLLATNDAVSKLLKSQGVNEKDLKAAIRDFRKGSTMHSQSGENQINAL
                     NKYAINLNERASSGKLDPVIGRDEEIRRVLHILSRRTKNNPILVGDPGVGKTAIAEGI
                     AHRIVNGDVPENLKSKTIFSLDMGALIAGAKYKGEFEERLKGVVKEVIAADGQIILFI
                     DEIHTLVGAGGGGEGAMDAANILKPALARGELRAIGATTLNEYQKYFEKDKALERRFQ
                     KVMVDEPGFEDAVSILRGLKERYENHHKVRIKDEAIIASVELSSRYISDRKLPDKAID
                     LIDEAAAKMRLEMDSLPEELDEIERRIRQLEIEREAIKREKDDKKVEELSKAIADLSE
                     ERNAIKAKWQQEKEVVEKIQQSKIDIEQYKLQAEQAEREGDYGRVAELRYGKIKEAEA
                     ASIAYEQELKKMDAGKRLMKEEVDAEDIAEIVAKWTGIPVAKMLESDRMKLLRLEEEL
                     HKRVVGQDEAIAAVSDAIRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKAISDFLFN
                     DEHAMTRIDMSEYQERHSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKA
                     HPDVFNILLQVLDDGRLTDNKGRTVDFKNTIIIMTSNIGSHIIQERFEGVNEDNLLAV
                     TATTRIEVMEMLRKTIRPEFLNRIDEIIMFKPLLRSEIRGVVEIQLHLLADLLAERDI
                     KLKFTPALITWLADEGFDPQFGARPLKRVIQKEIVNTLSKRILAGEVTAGSGAEIEVD
                     VAQGDVVFRNVVG"
     gene            12168..12341
                     /locus_tag="PLM90_04475"
     CDS             12168..12341
                     /locus_tag="PLM90_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88654.1"
                     /translation="MENTKKEKYEIKVNMKFLLLGLLFSAATVWMVFNLSQFFWIGLP
                     FSFTFLAKSIDAL"
     gene            complement(12465..13922)
                     /locus_tag="PLM90_04480"
     CDS             complement(12465..13922)
                     /locus_tag="PLM90_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HMZ88655.1"
                     /translation="MLLTFVIIYMVATIAIGVLASRLVKGSGDFVQAGRRLPPFFNAA
                     ALFALWFGSETVFGASSAFLEGGLLNVIEDPFGASLCLFLFGFFFVRKLYRMNLLTIG
                     DLFRIKYGQNVELISSFFMLLTFFGYIAAQYVALGIILQVVLLQTGINMSLPTAIILC
                     SIIVTAYTMIGGMWAVSITDFIQSILIVIGLIIVVIFVANEAGGVSQVIANAPENTFK
                     FLPNAEPIDITNWIGAWMVIGLGYIPSQDVFQRANSARSEKAAVNSTYLGAIMYLLIA
                     MLPLFIALAARVLYPDQDFSDSQSVLPHIVLDHTPLWIQILFFGSLLSAVLSTCSGSL
                     LAPASILAENFIKPLYKSNGKREMTDRKFLIVLRLSVVAIAFISFLITLPRQNIYELV
                     GESATFGMVSLLAPMTAGLYWKNINTQGALLSMFGGLVVYVFFQYFYPISIEAFIPAT
                     IVSFICMYAGRFIDEKKSPRYASTLANRFTKFFKD"
     gene            14080..15963
                     /locus_tag="PLM90_04485"
     CDS             14080..15963
                     /locus_tag="PLM90_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ88656.1"
                     /translation="MRKIILPFMLLAFAGTSCSHEPETSDAASKTDTMQFNDPHSLAQ
                     PTVAVGTHLDWHATVDFNTHTISGTAIWTLKSSPDAKEVIFDTRGLTISKVTTDGDNV
                     AAFTLGDTIPGQPWMGQALHIPISASTKTVHISYSSSPDAAALQWLSAEQTRDKKFPF
                     LFTQSQAILARTWVPCQDGPGVRFTFNAEVQVPTGMMALMSAENPQSVSADGIYRFKM
                     EQPIPSYLMSLAVGDLAFKSVGKETGVYAEPSYVDACAEELSDMQAMLESAEKLYGKY
                     QWGRYDVIVLPPSFPFGGMENPRITFATPTIIAGDKSLVALVAHELAHSWSGNLVTNA
                     TWNDFWLNEGFTVYFENRIMEALYGKDYADMLKVLEYESLVETVKEFGSDSADTHLKL
                     KLEGRDPDEGVSDIAYNKGAYFLRLLESKVGREKWDAFVNGYFTSNGFKVMTTEAFID
                     YLNKNLIEPNKAAFADVDINAWIYGPGIPDNCPKITSTRFEQVDAEVTKFKGGSAASS
                     LATSGWSTHEWLRFIYQMPDDISPEKMSELDKAFKFTGAGNCEIADAWYELAVRRHYT
                     ASYDAMDTFLNSVGRRKFLMPLYKAMMQTGQEALAKSIYAKARSGYHYVAQNSLDDLV
                     GKP"
     gene            15974..16996
                     /locus_tag="PLM90_04490"
     CDS             15974..16996
                     /locus_tag="PLM90_04490"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HMZ88657.1"
                     /translation="MTGTEQRKIIFIVGNSRSGTTMMRRVLGNHPDVYMLEELHFFEQ
                     LWSTNDKHRKMSREEAEQLASRLLFIQRDGYLTTMDLQKHANEAKAIIDSMQVDLFPH
                     EVLGAFLFHETRKNGRSIPCEKTPQDVFYIAEILELFPDARIVNMVRDPRGVLLSQKR
                     KWQRRAMGAGFMTKKEQRRLRINYHPITISKLWNSSIRAAAKYKGHAQVHNVNFEQLV
                     NASEAEVRSLCAFLGITFNANMLQIPQVGSSNEADKPDAFGIKTERAGNWEKGGLNKA
                     EIWFCQKICGENMHIYNYEVKPISANPFMILWYYLSFPVKIILAILFNLHRMKNIVEA
                     IKRRLS"
     gene            17047..18240
                     /locus_tag="PLM90_04495"
     CDS             17047..18240
                     /locus_tag="PLM90_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88658.1"
                     /translation="MSTGKKVLHITIHLPFPTNHGASLFNSYKLCTWLHRNHDLHFAC
                     FLKGDDVKYKDAFLEDTGIKNYYFEVLNVPRNGMNLIKSYLKGMTINMFRNYSADMHR
                     HIQSIAHEYDVIVAEHYEVMQYVPKDYKGKVVFRTHNAEYLIWSRFAEVETNPIKKLV
                     VSMEARRIKYWEFRYVKEADVVLGGTNDNEVHEPDPQKRALKFRDYLHIGDDSQITLP
                     IPDFEQLENSLVYVGTLTWEANVEGLLWFADGCWDILKERFPELKLYIIGKNPDQRLI
                     ELSKKYPDIIITGFVEDLEEYFMKCKVNIIPLRFGSGMKVKTINGLCRGIPMVCTTIG
                     AEGLKVTHDHDILISDTHAGFIQYVTELLNDNQKWNHIAHKSKATASKYYTWESLYKI
                     LEESI"
     gene            18401..19129
                     /locus_tag="PLM90_04500"
     CDS             18401..19129
                     /locus_tag="PLM90_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HMZ88659.1"
                     /translation="MVIDHRYKFVFVELPLTATSAISKEIREQYGCESYLNKHATYDD
                     YLRKANSEMRKYTSIGSVRNPLDQTVSMYFKYKNDLDQRFSTGRKRPGKWLRKSLAKN
                     RDSVRYQYIIDNQASFEQYFMKFFKWPYSNWSIVHHKKMNHIIRFEHLVEDYRKVFTA
                     VGVPITRDLPQANKTPEKKADFWSYYESDAAKRRAKFVFGPFMRYWGYSFPASWDHVK
                     EPAHAQLVYNLLNIPRRIYWRLLR"
     gene            19202..20428
                     /locus_tag="PLM90_04505"
     CDS             19202..20428
                     /locus_tag="PLM90_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMZ88660.1"
                     /translation="MRKLLLLLCLPTALYAQQPTKAEYRQSKSGYFQEVILTDINKAK
                     AEEAKDNRVFKIDASSVNAPKDPTKYTTVWHTNPVSQGNTGTCWCFSTTSFYESEVKR
                     NANIEVKLSEMYIVYWQYVERAKYFVANRGNMTFGEGSETNGTTWMIKQYGIVPYDSY
                     HGKTSTAGFYDHEPMFNEMNTYLQSIKTSNNWNEAEVVATIKSILNHYMGEPPTSVTY
                     NGKSYTPIEYTKNVLKINADEYVDFMSLMQKPFYAKAEYDVPDNWWNDASYYNLPINE
                     FMETLNTALKNGYSVSIGGDVSEPGWDSWNNIAVVPTWDIASNNIDDAARQYRFNTGA
                     TTDDHAMHLVGYTVQDGKTWYLFKDSGSGSRNCGAASPSFGYLYVSEDYVKLKMMTFT
                     VNKNAVKGALDKFAAK"
     gene            complement(20483..21169)
                     /locus_tag="PLM90_04510"
     CDS             complement(20483..21169)
                     /locus_tag="PLM90_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ88661.1"
                     /translation="MATIKILYVEDEANLGTIVSETLEQKGFDVRLIADGNLVLQACL
                     TFKPDIAVLDVMLPGVDGFTIGKNLRLQFPDLPIIFLTAKTQTKDVVEGFQSGGTDYM
                     RKPFSVEELIIRIQNQLRLRNVQQVASEIIRIGKFIYHPKKLLLENGENRWQLSSREA
                     EVLDVFSRYINRTIDRKTLLLEVWGDDSFFHSRNLDVYIRKLRDYFGSTNGVQIITLK
                     GKGYQFVVEN"
     gene            complement(21156..22619)
                     /locus_tag="PLM90_04515"
     CDS             complement(21156..22619)
                     /locus_tag="PLM90_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ88662.1"
                     /translation="MMSIKTTSSIGIRRIRALIALFCCAMLLLLMFTARWLQTEYNSE
                     EMDLHKELVQEFMSARDQVMDTVLTRSFIEPLLSNEGGFKIRHVSQDTITSGDSVTII
                     AKSFDTDTMIRITSFCDTCPTLSEANLTNIDSNNALMRGVKLFITEVKGDKGGKEFFD
                     RHIELKDTALLQSIYQQNLADQGLNLEIKWVHDSSKFTFPPPMFEYDSHLFNEPFKAQ
                     VDGYKSVILTTMVPQFLFAGLLITVVLVAFIFSYRNLRKQWQLAQLKDDLISNISHEL
                     KTPVATMKVAIEAMQEMDPVEKKEKIRSYLNMASEEVTRLDTLVNKVMQSVIGDGAHE
                     FFHTTLIDVTGIANGLTNYLQSKHVDINVSIPETPCYVMGDAFHLRSALQNLLDNAAK
                     YGGTKVSLDVSQGADMVQISISDNGPGIPETYHQKIFEKFFRIPTGNVHEVKGYGLGL
                     HYVKQVTEAAGGSISVNNNSDTGCTFTLLIPRADGNH"
     gene            22737..23483
                     /locus_tag="PLM90_04520"
     CDS             22737..23483
                     /locus_tag="PLM90_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HMZ88663.1"
                     /translation="MRKITLLLTMALSGLIAQAQTSGTIQYVSTRKLQINIQGDMPPP
                     PGLPSEIKLNTVLYFNTEASLYKNAENANDAMNVEQEAEGGGQIIMHMAPPENIMYCD
                     LTNKILTQQRDFMQRKFLIESPIDGSGWKLTGKQKMVLNYPCMEAIRVDSIDTVSAWY
                     TPVIPLSTGPLDYVGLPGMVMEVHVNNDMEVITATAVSKDVDASQIKKPKDGKKVTQE
                     EYDKIVEEKLKEMGVEPGPGGRVMIHIEND"
     gene            23494..>24986
                     /locus_tag="PLM90_04525"
     CDS             23494..>24986
                     /locus_tag="PLM90_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMZ88664.1"
                     /translation="MKKFAIYLVCIFLSVGLNAQVQNLSGHVFNSESKPVEYATVALL
                     QPQDSTLAFFAITDAEGAFSIKSVKAGTYRLQISFIGFDTYDTLLTTPLPNTNLGVYI
                     LQTAAIGLDAVQVTAERIPIQILGDTLQYNANSFKTAPDASAEELLKKLPGVEVDASG
                     NIKAQGEDVQQVLVDGKEFFSSDPTVATKNLPADAIEKVQVYDKTSDDADFTGVDDGQ
                     RSKTINLVLKDGKKSMWLGNAQAGYGTDDRYQTSAKLYRFTKTNQIAALGMLNNVNDF
                     GFSINDYIDFSGGIGNMMRSGGFQISSDNDVPVNFGQSVDGKITSGAAGLNYTYEPDE
                     DHRFNVSYLGNGYLRNLTQNTRTEQFSELGNFETIDSLNEETEKYFNRINFNIRTKPD
                     TLRSFIVTGNASMNMGDVTSSGFSANMFEDSLTNTLNNAAYSQNNNFSGTAAASYIRK
                     TNTKWKYFKVKANGSGGTSLNEENWNNLTAYFADGLTIADNSARTDDA"
BASE COUNT         7644 a         5040 c         5319 g         6983 t
ORIGIN      
        1 cgcatttgag cgaccgtttc tttttgaaga agaattggtg ccttacgacc aggaatttgc
       61 gcggtgcaga tgatcacatc ggctttagaa gcatgttctt gcactttagc ctgttgacgt
      121 tttttaaagt cttcactctg ttcaaccgca tatccgcctg cacttttatc ttcggtagca
      181 ccttccactt caatgaattt ggctcccaac gaaagcactt cctcgcgggc tgctgtgcgc
      241 acatcgctgg cttctacaac ggcgccgaga cgctttgcag tagaaatagc ctgcaatccg
      301 gcaacaccgg cacctaatat caccacttta gcaggcgtaa tagtaccggc agcggtcatg
      361 aacatcggga agaattttgg caaatggctt gcggcagtca acacagcctt ataaccggct
      421 acggtagcca ttgagctgag aatatccatt gcctgagcac gggtagttcg agggattaca
      481 tccaagctga aagctgaaac gccgttttca agacaagcgc tcacaaacga ggtgtttacc
      541 aacgggttga agcctccgat gagcactttt ccgcgcattt tggcgagttc atcacttccg
      601 acaggatgaa tcttgatcag catatcgctt ttggcaatca cctccgctct ggaagcgatt
      661 tcggcacctt tcgcgacata gtcagcgtcc ggtgaccctg ctgccaatcc ggcattggac
      721 tcgataagta ttttctctgc ttttaaggct tttagagagg gaatgtgttc agctgcaatt
      781 gccactcgct gatccgactg accctctccc agaattccga tgatcatgtg aagatttgga
      841 tgattaggac cgcaaagata gttaaaaatg ggcataagcc tgccgttttg accttcaaaa
      901 tcaggtatcg tgtagctttg tataacagga tatcaacaag tcctgtaatg cgatgcaaac
      961 attcaaaagc gacctttcag acaaggagtt tcccattaag gaaatggttt atgcaactgc
     1021 tgtgcgccca accatcatga cgcttatcaa ggccgatcac ccggcttttg atgaaagtaa
     1081 gggcttgctt tcattgagcg agttgagttt ttacagagag aaatacattg cggattacct
     1141 caaaagtgaa atagctgact tgagtgacct ggagcatacg gtagtgaaaa gcttgcagga
     1201 caattcactg ctcagcgacc gtgcagattt ggaaggagat acaaatatca caacaggtca
     1261 gcgtcttgca gataatgttg cacgatttgg aggtagttgg cgatttatca ttctgtttgg
     1321 gctgtttctg gcaatatgga tcatgatcaa cgcattgttg ttatcgaata aaggatttga
     1381 tccatatccg tttattttac tcaacctgat gctctcgtgt ttggctgcga ttcaggcgcc
     1441 actgattatg atgagtcaaa accgtcagga ggaaaaggac aggcagcggg caaagaaaga
     1501 ttatatgatc aatctcaagt cagaaatgga gatacgcctc ctacatgaaa aactcgatca
     1561 cctgctcatg catcagcaac agaagctgtt agagattcaa caatttcaga ttgagatgat
     1621 gaatgatatt tataataggt tgaaataaac taatttccat ccatcaaaac aacaatatgc
     1681 ctgaagacga attttacgaa gatagcgtgc aaacaatgga ggattttatc cgatatgtgc
     1741 atgaattgag aaaagatttg aagacaaatc cggaagattt cgagaacaat actttgccat
     1801 tatttaagga actcatctca aaaatacctt tggcgtcttt tctgtgtctt cgccctttat
     1861 ccttcgttac ttgaattgcc cgtaccaccg ccagtatggg tcgcttcaag tgccttggct
     1921 aaagtgcgaa tacttgaaaa tactaatcca aatctatttt tgagatgagt tccagaagct
     1981 attgccgact atacagagca tgggcttaga ggcagatact atttctttaa ggagcatttt
     2041 gatccggaaa agccctcctg gaatatgttt gccaggattc tggcaggtgc ttcggtgttt
     2101 gaatgagcaa gtacaaaaca tcatgtaact tcgatcagat acttgtttcc cgggtttatc
     2161 gttttagtaa ttaaactgca catgaagacc ttttgtacat tgatatgctt tttgttattt
     2221 gtcctaccgg cagaggcaca acaggattcc gtatttctga aataccctgt agatatagat
     2281 agcctgggca aagcaattta ttcggtggat accatattat ttcgagcata tcatgatggt
     2341 gatatcctta tcggaaccac tgttattccg ggtacgaata attccatgaa cttaatgaat
     2401 catgggtata ggcttcacaa tattcgcctt atgccctgcg atggtggcga tgtgtttgaa
     2461 gtggtttcgc gggttaatgc agtaagggaa acagattcta cactggaagt ggatgtcacc
     2521 atcacagata attgttgttt taatttttta tgtgatgcaa gaattgatga aattaatgtc
     2581 atgcatctgt tttacacagg ttatggcatg aattgctctt gtttctgttg ctttgggttg
     2641 acttatgttt tcgaacgttg gaattttacg aatgatcctt tgattgatgc cgtcatgatt
     2701 gctgatgata aaaagagttt atataatata tcccgtgatg cgaaggatta aactgttgtt
     2761 gattgctttc gtatctatta tttcacttcg tgcgcaaccc gaattggaat tttgcagggt
     2821 tgctcctatt gtttatttcg attccgtcga taataaacca atgctgaatt tacctgtagg
     2881 cggaaagtgg attgattcac tagagttttc tttaaatggt aattataaaa attcatttgt
     2941 ctctgtagat attggcgtgc aaggctccgg agctgaaatt ttgcgcgaat cggattcctt
     3001 gtggcggatt tctttgtttg catactttga tttttcatca gattcggata cagcgcaaga
     3061 aattgaagtg gcgcgattga ttttgacaaa aaacgaagcc ggatacattt gcaaaactga
     3121 cctttctttt cgcccggacc cggtagagtt gaaaggtgaa caattgcata acttgttttc
     3181 ctcatgtgcc aacaacaacc tatataaatt atcagcattc gatattggtg aaagcgaatg
     3241 ggataccctt agtttactca tcaacgattt gtttgttttg agtttgcatg gagatgcatt
     3301 ggcattgaaa gaattcaatg acatgctaat attatttcct gccgttaaga tgtatgaatt
     3361 gcatcattac tatatgagcg cccgtgcagt gctctgttta tatggcatga agtgattaat
     3421 tcaacgttta tatcgtaact ttgcatttca attaccacta tgtttcgcaa atcgatacca
     3481 ctctttataa tcgtgctgtt tatttcatcc tgcaccatga ttagaaacac taccaatact
     3541 tccccgggcc aggaaccttt gtttttatca aaatggactt tggataattt gcatggatcc
     3601 actataagca ttgagtcgca gccatatttg ttgtttacgg ccagcgaaac attcagggtg
     3661 agcgggtttg caggctgtaa cctgatcacc ggcggattta ctttgagtgg agtggatagc
     3721 atcgatttcg gtgaaattgc ctctaccttg atggcttgtc cgcagtatgc taccgagcaa
     3781 gattttttga cagcactgca agaggcaaac agatggatga tctcaggagg tcagctattt
     3841 ctgttacaag gatctgatgt tgtggcttct tttaccggat caacaattga tgtggcagaa
     3901 gaggccggga atatggctgt aaacggcaca tgggaattgt cttatatcag tggtccacgc
     3961 atcgcatttg atgccttata tccccagcaa aaaccaactt tagtaatatc catgccaaat
     4021 acgaaggcta ccggtaactc cggatgcaat tcgttttctg tggatgtgaa aatcaaggat
     4081 aaaaccatct tattcggacc gattgccgct acaaaaatgt attgtgaagg ttcgggtgaa
     4141 tcaacctatt tcgataacct cggaaaggtg gatacctggc aaatggatga taataacaac
     4201 cttgctttga tgattggcga tgttgtgatg atgcgattta ttcgcaaata aggtttccgg
     4261 cttcggaagt actacctttt ctgatcatta catgataatt ttgaaggatg aaatccatcc
     4321 tgactctgct atgtggtgta ttgttatcta cttccttacc tgcagtaaca gtaacatcac
     4381 ctgatcgaac attattgttg aatgttgatc tggataaatc cggcgcactg cattattccc
     4441 tctctcgcaa tggagcagtg gtgatagcgc catcgctcat gggattctct ttgaaagatc
     4501 ttggtgaact taccgccgat tttgaagccg tagataccgt atacaattcc atcgacacat
     4561 actgggaacc ggtttggggt gaagtggcga aaattcatga caatcacaat gaaatgacct
     4621 ttacggttcg acacaaaaaa acaggtatcg taatggaggt gattttcagg atgtttaacg
     4681 acggtatggg tttcagatac caatttccgg tgcaatctta tatgaagtat tttacggtgt
     4741 tgaatgagaa aacacaattt tgcttaaatg gcgatcatca ggcatggtgg ataccgggcg
     4801 attatgatac caacgaatat ttttataccg aaagcaaact cagcgatgtg catgcaatta
     4861 ccgcatccac cagaaacaga gaaattgcaa cgcgttctcc aataggcgat aattatgtgc
     4921 agtcgcccct tatgatgaaa tcatctgatg gcttgtatat caatattttt gaagcagcac
     4981 ttgttaatta tcctgccatg catcttgaag tagatcgtga gaaacatatt ttaacttccc
     5041 gccttgctac agatgccgtt gggaattgcg catatttatc tactccttgt catacgccat
     5101 ggcgcacagt tattgtgagt gataaagcga cagatatttt aggatctcat attattctta
     5161 atttaaatga accttgtgcc attgccaata ccgattggat taaaccaatg aaatttgttg
     5221 gcatttggtg ggaaatgcat gttggcacag catcatggaa ttacagtgat gtaaataata
     5281 ttaatctgga aaccaccgac tggaccaaat tgccacctaa tggtcaccat ggcgcaacaa
     5341 cagagcgcac taaatactat atcgacttcg cggcagcaaa tggatttgac ggtgtgttag
     5401 ttgaaggctg gaatgtaggt tgggaagatt ggtttggcaa ctggaaagaa aatgtattcg
     5461 attttgtgac gccatatccc gattataatg tgcaggaatt atcagcttat gctaaatcaa
     5521 aaggcgttag tctcatcatg catcatgaaa cctctgcatc ggtaactaat tatgaacgca
     5581 gattgcagca agcaatgcaa tttatggtga gcaatggata tcctgccgta aaaaccggtt
     5641 atgttggtcg tataattcca agaggcgaat atcacgacgg acaatggatg gtaaatcatt
     5701 atgtgtatgt ggcacaggaa atggcaaaac atcatctgat ggttgatgcg catgaacctg
     5761 ttcgtccaac cggtttacaa cgcacttatc cgaactggtt ggcatgcgaa gcagcgcgcg
     5821 gaaatgaatt taatgcatgg agccagggta acccgcctga acatgaaaca atattgccat
     5881 ttacaagatt aatgggcgga ccaatggatt atactcccgg cattttcgaa gtgcagatgt
     5941 caacctatgg acagcaaaca ggtaatcagg tgcataccac acttgccaag caattagcat
     6001 tatatgttac aatgtattca ccattgcaaa tggctgcaga tatacctgaa aattatttaa
     6061 agtatgccga tgcatttcaa tttattaaag atgtagctgt agattggcag gatacgaaat
     6121 acctggaagc agaacccggc gattatctca ctgttgcaag aaaggaaaaa ggtggcagaa
     6181 actggtttgt tggtgccatt acagatgata aggcacgaag aacatccatc aaactcgatt
     6241 tccttgacga caagcaagta tacatcgcaa caatttacgg agatgctgaa ggagctgact
     6301 ggaaaacgaa tccaaaggca tatatgatca cacaacaaaa agtgacaaat atgtcgacca
     6361 taaaattaga actggctccg ggtggaggtg cagcagtgag tattatgccg attcaggaga
     6421 attgatggcg tttaatattt gacaatttct gcaatctaag ttttgttatt tgtttgcacc
     6481 tgcgcaacat ctcacatatg cctgttagat aacaattgtc atattatttc actattgtct
     6541 aagggagcta aaccatatat tagcaaaaat aaaacacggt tcagcacaat ctgtgtgcga
     6601 tgctgttgca gaaggttgat aaagtgaagt ggttgttgaa ccatttgcga caaatgcatc
     6661 ctgtgcaata atactattct gataattaac cagttcgtcg ccaccgcaat aatacatgat
     6721 catcggaatc tgtggcgtcc agtcggtgag gtcgttttca cgcaatgcta cgcggaacgg
     6781 gttattcgga ttgttaatga aatcctccag cacttcaggt tttatgatat ttttgggaat
     6841 aacgggcatt acagcatcta tttcccaacc actatgtgtg ccatcaaaaa gtgaaggtaa
     6901 tagggtgtcg taaggtgatg cgagataatc ggcataatta tcatacatct tataaatgtc
     6961 attgtacccg aacattaaat acggtaaata aaacggagca ccatacggag cgtcttgaag
     7021 cacatagtca gtttgtgcgc cactcaaatc ataagggccg ctcattggcg cacttgccgt
     7081 tatcgtaaat tcatctgcat aatcctcttg gatagctttg gttgctgcca ttgccgcatg
     7141 cccaccctga ctgtaaccga aaataaataa ttgattattt aaggaaacac cttcatttgc
     7201 acaaagtgtt ttagttgcac gaagaagatc aattcctgca gatgcttctg ttgctgaatg
     7261 acaatatgga tgtcgccccg gtgaatcgcc catgccgaga taatccggta aactggtaac
     7321 aaatccgttt gcaccataca acatacctaa cactaattca tcactttcat aagacggcac
     7381 ttcaaatcgt tcaactaccg ttccgtgcat atataaacac tgagctgcac cgtttgcaac
     7441 accgtaaggg attactacag caccggatgc ctgagtctca tttcctgctg catcaattgt
     7501 gcgatatacc acacgataaa ttttgatatt ataatcattt ggatacgtca gtaatgaagg
     7561 atcataatcc gataaagcag aatctgtttg tgtaattgac cagtcatgta acaatgtgct
     7621 ggaaattatc tcacctctga agctatcgtc attcaccatt ggcggatcaa cggcgcatga
     7681 tgcaaaaaac attgaggcag taaacagata aaggatgctc ctgaagtgtg aaagggtagg
     7741 tctcatgtat tatataactt atcttaccaa agataagcta aggtattgaa attgagatta
     7801 ccttatatcg ctttgctgaa agtattgccc tgcctgcttc cgctatgaag atgcaaatca
     7861 atagcagaac agatattcct gataaataag cggcctgagg gtaatatcga aattttgccg
     7921 ccttctatac tgattaatcc atcttctaca aatcccgaca gtctttccat gatagcgtca
     7981 aatgtgccgg caggtaattg ttcttccaca agtgtggtgg tgtcgcgaca cattaaatcc
     8041 agaatatgtt tgcggataag ttgatctgtt tcattaagat tatgcccgtt tattattggc
     8101 agtcgccctg aagcaatagc atcctgatat gcttcaacac ctttttcatt ctgtgcaaat
     8161 gcatcccagg tatcactgat ggcagatgca cctaaaccaa ttaccaattt gtgattagta
     8221 gttgtataac ccatgaaatt gcgatgtaat ttacctactt ggtaggcgag cattaaagca
     8281 tcagtaggta aagcaaaatg atccatacct atatgcgaaa atcctaattg gtgtaaatag
     8341 gcactccctg tttgatacat ttgtaatttt tcttcagcaa caggaacatc ttcatctgta
     8401 tatctgcgtt gacctttgct tttccatggc acatgtgcat atgaataaaa tgcaatacga
     8461 tctggcatga gttccttcac cttttccatc gtcaatttga tactgtcgtt ggtctgttta
     8521 ggtaacccgt atatcaagtc gatattcacg ccggtaaagc ctaaagctct ggccgtttta
     8581 atcacatgtt gtgtttgctc aaatgtttga gggcgattaa taataaattg cacaacaggg
     8641 tcaaaatcct gaacacctaa actgatgcga ttaaatccaa cctcctgcaa tgctacaaga
     8701 tgttgttcat ttgtataatt tggatgtact tcaacactaa aggcatggtc attcgcaaca
     8761 attgcatgtt tagtaatgcc attgatcaat tgtttcaaat gctccggtga aaagaaagtg
     8821 ggtgtgcctc ctcctaaatg tatttcacga ataacaggag tgtctgccaa caatgcacga
     8881 tacatctccc attctgcaag cactgattcc aaatagggta tttcaactgc atgattttta
     8941 gtgatgcgtt tattgcatgc acaataggtg cataattcct cgcaaaaagg taggtgtata
     9001 tatatacaca gttcttggtt ctcttgtttg aatgagcgaa ttacagctga tttccaatta
     9061 tcaccttgaa ttgtttgaaa atcccacgac ggcactgtag gataactggt gtaccgaggt
     9121 accgcaacat catattttga aagtaacctg aattgatcta tcgacatgaa tgattatcgg
     9181 tctttatgag gaatgcattc cctcaatcat tgcacaaaga tacaaccacc ggtctattag
     9241 attgttaaag aattgtcatt caaaacctgg gtgaaaggat ttttgttgta tgctattgac
     9301 aacttgacct ttatgcttga gatggcctga cagcctgtcc tttggggcta ttgaaacagg
     9361 acatattggc gtaaaagttt tggtattcag acgaagtatg ttttttgcta tatgctttgc
     9421 attatccctt aaaataagac tgatatgaac ttacaacaat ttactatcaa agcacaggag
     9481 gtgatccagc aggcacagca aatggccatg caaatggatc atcaatatat tatgcccgag
     9541 catatcctga aagcactgtt atctgatgat gaggttgcag gatatctgct taagaaatta
     9601 caggctaacc cagtttatat tcaacaaaaa ttagacgagc agttagctgc agttgcaaag
     9661 gtggcttcgg gacaaggagg tcagtatatc tctcgcgaag cgaatttggt tattgcaaag
     9721 agtcaaacat ttttgaaaga attcaacgat gaatttgttt ctgttgaaca ccttttgtta
     9781 ggattactgg caactaacga tgccgtgtca aaacttttaa aatctcaagg tgtaaacgaa
     9841 aaagacttaa aagccgccat tcgggatttc agaaaagggt caaccatgca ttctcagagc
     9901 ggcgagaatc aaataaacgc actgaataaa tatgcaataa atcttaacga gcgagcatca
     9961 agcggaaaac tggatcctgt aatcggacgt gatgaagaaa tccgtcgtgt gttgcatatt
    10021 ctttcccgac gcacaaaaaa taatcctatt ttggtaggtg atccgggtgt cggaaaaact
    10081 gccatagccg aaggaatagc acatcggatt gtaaacggtg atgtgcctga aaatctgaaa
    10141 tcaaaaacga tattttccct cgatatggga gcattaatcg ccggtgcaaa atataagggt
    10201 gaatttgaag agcgattgaa aggcgtggtg aaagaagtga ttgccgctga tggtcagatt
    10261 attttgttta ttgatgaaat acataccctt gtaggtgccg gaggtggtgg tgaaggagct
    10321 atggatgctg caaacatttt gaaaccggca ttggcaagag gagaattacg tgctattggc
    10381 gctactaccc tgaatgaata tcaaaagtat tttgaaaagg ataaagcact tgaacgaagg
    10441 ttccagaaag taatggttga tgaacccgga tttgaagacg ccgtttccat cctccgcgga
    10501 ttaaaagagc gttatgaaaa tcaccataaa gtgcgcatta aagatgaagc gattattgca
    10561 tctgttgaat taagttcgcg atatatttca gatcgtaaat taccggacaa ggcaatcgac
    10621 ttgattgatg aagctgcagc aaaaatgcgc ctcgaaatgg acagtctccc ggaggaattg
    10681 gatgagatag aaagacgcat ccgccaattg gaaattgaac gtgaggcaat caaacgtgaa
    10741 aaagatgaca agaaagtaga agaattgtcc aaggccattg ccgatttatc tgaggagcga
    10801 aatgcaataa aagcaaagtg gcagcaagag aaggaagtag tggaaaaaat acagcaaagt
    10861 aaaatcgata tcgaacaata taaactgcag gccgagcaag cagaacgcga aggtgattat
    10921 ggcagggttg ctgaattgcg atacgggaaa attaaagagg cggaagctgc ttccattgcc
    10981 tatgagcagg aattgaaaaa aatggatgcc ggtaaacgtt taatgaaaga ggaggttgat
    11041 gccgaagata ttgccgagat cgttgctaaa tggacaggaa tcccggttgc aaaaatgctc
    11101 gaaagcgaca gaatgaagtt attgcgactt gaagaagaat tgcacaaaag ggtagtgggg
    11161 caagatgaag ctatagctgc ggtatcagat gctatcagac gcagtagagc aggactgcaa
    11221 gatccgaaaa ggccaatagg ttcatttatt tttctcggaa ctacaggtgt aggtaaaact
    11281 gaattggcaa aagccatttc cgacttctta ttcaacgatg agcacgcaat gacacgcatc
    11341 gacatgagcg aatatcagga acgtcatagt gtgagcagat tgattggcgc tcctccggga
    11401 tatgttggct atgatgaagg aggtcagctt acagaagccg ttcgtcgaaa accatatagc
    11461 gtggtgttat tggatgaaat agaaaaagcg catcccgatg tatttaatat cctgttgcag
    11521 gtattggatg atggtcggct taccgataat aaaggtcgca ctgttgattt taaaaacact
    11581 atcatcatca tgaccagcaa tatcggttca catatcattc aggagcgatt cgaaggtgtg
    11641 aatgaagata atttgctggc agtaacggca accacaagaa ttgaagtaat ggaaatgctt
    11701 cgcaaaacca tcagaccgga atttcttaat cgtatcgatg agattatcat gttcaaaccg
    11761 ttgttgcgaa gcgaaatcag aggcgtagtt gaaattcaat tgcatttgtt ggccgatttg
    11821 cttgccgaaa gggatataaa actgaaattc actcctgcat tgataacctg gctcgccgat
    11881 gaaggttttg accctcaatt tggagcccga ccactgaaaa gagtcatcca gaaggagatt
    11941 gtcaataccc tctcaaaacg gatattggcg ggagaggtaa ctgccggcag cggcgccgaa
    12001 atagaggtgg atgtagcgca gggcgatgtg gttttccgta atgttgttgg gtaaaatcac
    12061 ctgccaaatg cccgattaca tagggtttgg gcaatttgtg ggttttccac caccaaggtt
    12121 ttgaaatccg attttgatgc gtaactttgt cgggtcaatt tacagctatg gaaaatacca
    12181 aaaaagaaaa atacgagatc aaggtaaata tgaagttcct cttgttaggt cttcttttta
    12241 gcgccgctac agtatggatg gtgttcaacc taagccagtt tttctggata ggattgccat
    12301 tctcgttcac cttcctcgct aagtcaatcg acgcattata aattaagtta ccttatcaaa
    12361 agatataggc aattccttaa cgggagttgc cttttttttt atgccacaaa taccttatta
    12421 atacacaaac taatcagcaa aaagttattc ggagagcgtc taaatcaatc cttaaaaaat
    12481 ttagtaaacc tgttggcaag ggtcgatgca tagcgcggtg atttcttctc atcgataaat
    12541 ctgccggcat acatacaaat gaagctgaca atggttgcag gaataaatgc ttctatgctg
    12601 ataggataaa aatattgaaa gaaaacatat acaaccaggc cgccgaacat actcaatagt
    12661 gcaccctgcg tgttgatgtt tttccagtat aatccggctg tcattggtgc aagtaaactc
    12721 accatgccaa aggtggcact ttcgccaacc aattcatata tgttttgtcg aggtaacgta
    12781 ataaggaaag aaataaatgc tattgcgaca acactcaatc gcaatacgat aagaaatttc
    12841 ctatcagtca tttctcgctt cccattcgat ttgtatagtg gctttataaa attctctgct
    12901 aatatgcttg caggagccaa caatgaaccg ctgcatgtac ttaatactgc cgataataag
    12961 gaaccaaaaa ataatatctg aatccatagt ggcgtatggt ctaaaacaat atgcggaagc
    13021 acagattgtg aatctgaaaa atcctgatca ggataaagaa ctctcgctgc aagtgcaata
    13081 aacaatggca acatggcaat aagcaaatac atgatggcac ccaggtaagt tgaatttact
    13141 gcagctttct cactgcgcgc cgaatttgct ctttgaaaaa catcttgcga tggaatatag
    13201 cccaatccga ttaccatcca tgcaccaatc caattagtaa tgtcaatcgg ctcggcattc
    13261 ggtagaaact tgaacgtatt ttcaggtgcg ttggcaatta cctgcgatac accaccggcc
    13321 tcatttgcca caaaaattac cacaatgatg agtccaatta cgatgagtat actttgaata
    13381 aaatcagtaa tcgaaaccgc ccacataccg ccaatcatgg tatacgcagt tactattatt
    13441 gaacagagaa tgattgcagt tggcaacgac atgttgattc ctgtttgcaa taagaccact
    13501 tgtaaaataa tccccaacgc tacatactga gcagctatat atccaaagaa tgtcagcaac
    13561 ataaagaagc tggaaatcaa ctcaacattt tgaccatatt ttatgcgaaa cagatcacca
    13621 atcgtaagca gattcattct gtaaagcttt cttacgaaga aaaatccaaa taggaaaagg
    13681 catagcgatg ccccgaaagg atcttctatc acgttgagca acccaccctc aagaaatgca
    13741 ctggaagcac caaacaccgt ttcgctgcca aaccataggg caaacagcgc tgcggcattg
    13801 aaaaatggag gtaatcgcct tcccgcctga acgaaatcgc cggagccttt taccaatcta
    13861 ctggctaata caccgatggc aatggtggcc accatgtaga tgatcacaaa ggtgagtaac
    13921 atctctctgg gaagggtatc tggttgtagt gaaaaatttt agcggtcaaa aataaaaaaa
    13981 tcgtcgaaac tgcccaatag cctaaataaa catattgttg atttttctca cctgtgaaag
    14041 cgcctaagga atccgtaaat ttgccaaata cttcaaggca tgcggaaaat cattctacct
    14101 tttatgttac tggcttttgc gggaacttcc tgcagccatg aacccgaaac gagtgatgct
    14161 gcatccaaaa ccgatactat gcaattcaac gatccacact ccttagcaca acccactgtg
    14221 gctgtcggca cgcacctcga ttggcacgct acagtagatt ttaacacaca tactatttca
    14281 ggaaccgcaa tctggaccct caaatcatct cccgatgcca aagaggtcat cttcgatacg
    14341 cgcggtttaa ccatctctaa ggttaccact gatggcgata atgttgccgc attcaccctt
    14401 ggcgatacca ttccgggaca accgtggatg ggtcaggctt tgcatattcc catttcggca
    14461 tcaacaaaaa ctgttcatat ctcctatagc tcatcgccag atgctgctgc tttgcaatgg
    14521 ctctcggcag aacaaacacg cgataagaaa tttccctttc tgttcactca atcgcaagcc
    14581 atccttgcca gaacttgggt gccttgtcag gatggaccgg gtgtccgctt tacttttaat
    14641 gccgaagtgc aagttccaac aggaatgatg gccttaatga gcgccgaaaa cccacaaagt
    14701 gtaagtgccg atggcatata tcgcttcaaa atggagcaac ccattccttc gtatttgatg
    14761 agccttgccg ttggcgatct ggcatttaag tcggtaggta aagaaaccgg cgtgtatgca
    14821 gaaccatcct acgtcgacgc ttgtgccgaa gaactcagcg atatgcaggc tatgctcgaa
    14881 agcgcagaaa aactttatgg caaatatcaa tggggacgat atgatgttat cgtgcttccg
    14941 ccttcattcc cattcggagg gatggaaaat ccacgaatca cttttgctac accaacaatt
    15001 attgccggcg ataaaagcct tgttgcgctg gttgcccacg aattggcgca cagctggagc
    15061 ggaaatctcg tcacaaatgc tacttggaac gatttctggt tgaacgaagg cttcactgtt
    15121 tatttcgaaa accgcatcat ggaagcactt tacggtaaag attatgccga tatgctgaaa
    15181 gtgctggaat atgaatcgct ggttgaaaca gtgaaagaat tcggtagcga ttccgccgat
    15241 acccacctta aactcaaact cgaaggtcgc gatcctgatg aaggcgtcag cgacatcgcc
    15301 tataataaag gtgcttattt tcttcgtctg cttgaaagta aagttggtcg cgaaaaatgg
    15361 gatgccttcg taaatggtta ttttacttcc aacgggttta aagtaatgac caccgaggca
    15421 tttattgact acctgaataa aaacctcatc gaaccaaata aagcggcttt tgccgatgta
    15481 gatatcaatg catggatcta tggtcccggc ataccggata attgtcctaa gatcacctca
    15541 actcgatttg aacaggtaga tgctgaagtg acaaaattta aaggtggtag cgcagcttct
    15601 tcattggcta caagtggatg gagcacacat gaatggctcc gatttattta tcaaatgccg
    15661 gatgacatca gcccggaaaa aatgagtgaa ctcgataagg catttaaatt taccggcgcc
    15721 ggaaattgtg agattgcaga tgcatggtac gaacttgctg tacgcagaca ttatactgct
    15781 tcatacgatg caatggatac cttcctgaat agtgtaggaa gaaggaaatt cctcatgcca
    15841 ttatataaag ctatgatgca aacagggcag gaggcactcg ctaaatctat ttatgccaaa
    15901 gcacgctccg gatatcatta tgtagctcaa aattcactcg acgatcttgt agggaagcct
    15961 taattattat tgtgtgaccg gaactgaaca aaggaaaata atttttattg taggcaactc
    16021 acgaagtggt actacaatga tgcgtagggt gctcggaaat catcctgacg tatatatgct
    16081 tgaagaattg catttcttcg agcagttatg gagtaccaac gataaacatc gcaaaatgtc
    16141 gcgcgaagaa gctgaacaat tggcttcgcg attactcttt attcaacgag atggttacct
    16201 cacaaccatg gatttgcaaa agcatgccaa tgaagcgaaa gcaattattg atagcatgca
    16261 ggtggactta tttccacacg aagttttagg cgcattttta tttcacgaaa cgcgtaaaaa
    16321 cggaaggtct ataccttgcg aaaaaacacc gcaagatgta ttttatatcg ccgaaatatt
    16381 agaattattt cccgacgccc gcatcgtaaa tatggtgcgt gatccgagag gtgtgctgct
    16441 cagccaaaaa agaaaatggc agaggagagc tatgggtgca ggatttatga cgaaaaaaga
    16501 acaacgtcga ctgcgtataa attatcatcc tatcaccatt tcaaaattgt ggaattcgag
    16561 tatccgcgca gcggcaaaat ataaaggaca cgcgcaagta cataatgtga acttcgagca
    16621 gctagttaat gcttctgaag ctgaagtgcg cagcttgtgt gcgtttctcg gtataacatt
    16681 taatgccaat atgctgcaaa ttccccaagt aggttccagt aatgaagctg ataagccgga
    16741 tgcttttgga attaaaacgg aacgcgcagg aaattgggaa aaaggcggtt tgaataaagc
    16801 agaaatttgg ttttgccaga aaatatgcgg tgagaatatg catatttata attatgaggt
    16861 gaagccgatt agcgcaaatc cattcatgat actttggtat tatctctcat tccccgtgaa
    16921 aattattttg gcaattttgt ttaacctgca tcgcatgaaa aatatcgttg aagcaataaa
    16981 acgaagattg tcgtaaattg ttttcatctt ctattaacaa tatccctacc aaaaatattt
    17041 attgttatgt caaccggaaa aaaggtatta catatcacca tacacttgcc gtttccaaca
    17101 aatcatgggg cgagtctttt caattcttac aaattatgca cctggttgca tcgtaatcat
    17161 gacctgcatt ttgcttgttt tctaaaaggt gatgatgtga aatataaaga tgcctttctg
    17221 gaagatacag gtattaaaaa ttattatttc gaagtgctga atgtgcctcg aaatggcatg
    17281 aatcttatca agagctattt gaaggggatg accatcaata tgttcagaaa ttattcagcc
    17341 gacatgcaca gacatattca atctattgcg catgaatatg atgtgatcgt tgctgaacat
    17401 tatgaggtga tgcaatatgt tccgaaagat tataaaggaa aagtggtctt tagaacacat
    17461 aatgcagaat acctgatttg gagccgcttc gctgaagtag aaaccaatcc aattaaaaaa
    17521 ttggtggtaa gtatggaagc acggcgtatt aaatattggg aatttaggta tgtgaaagaa
    17581 gcggatgtcg ttctgggagg caccaacgac aatgaagtgc acgagccgga tccgcaaaag
    17641 cgagcactca aattccgcga ttatttgcat atcggtgacg actcacagat cactctgcct
    17701 atcccggatt ttgaacaatt ggaaaatagc ctggtatatg ttggcacact cacatgggag
    17761 gctaatgttg aaggcttgtt gtggtttgct gatggttgct gggatatttt gaaagaacgt
    17821 ttcccggaat tgaaattata tattatcgga aaaaatccgg atcagcgact gatcgaattg
    17881 agcaaaaaat atcctgatat catcattacc ggtttcgtgg aagatctgga ggaatatttc
    17941 atgaagtgca aagtaaatat cataccgctt cgtttcggct caggaatgaa agtgaaaact
    18001 atcaatggac tttgtcgcgg tatccctatg gtttgcacta ccattggtgc tgaagggttg
    18061 aaagttacac atgatcacga tattcttatc tcagacacgc atgcagggtt tattcagtat
    18121 gtcacagaat tgctgaacga caatcaaaaa tggaatcata tcgcacataa atccaaagca
    18181 actgcatcga agtattacac ttgggagtct ttatataaga ttttggaaga gagtatctag
    18241 agtcaagctc gcctctggcg ggtatcaagc ccaaaaacca ctttgcgcct ctgcaacttt
    18301 gcatctttgc gcgaacccaa acacaaatca tattgtcttc ttttgagttg cacaagtaat
    18361 atcatcattc cccatccatc aaatttccta actttgcata atggtaatcg atcatcgata
    18421 taaatttgtt tttgttgaat tgccgttaac agcaacttcg gccatcagca aagagatacg
    18481 tgaacaatac ggctgtgagt cgtatctcaa caagcatgct acctacgatg attaccttag
    18541 gaaggcgaat tccgaaatgc gcaaatacac ctccattggc agtgtgcgca atccgctgga
    18601 tcaaaccgta agcatgtatt tcaaatacaa aaacgattta gatcaacgct tttcaacagg
    18661 aagaaaacgc ccgggtaaat ggttgcgtaa atcacttgct aaaaacagag attccgttcg
    18721 ctaccaatat atcatcgata atcaggcatc gtttgaacaa tacttcatga aattcttcaa
    18781 atggccttac agcaactgga gtatcgttca ccacaaaaag atgaaccata tcatcagatt
    18841 tgagcatctg gtagaagatt accgcaaggt attcacggca gttggcgttc ccattacccg
    18901 cgatctgccc caggcaaaca aaaccccgga aaaaaaggcc gatttctgga gctactacga
    18961 aagcgatgcc gccaaacgcc gcgccaaatt tgtgtttgga ccatttatgc gctattgggg
    19021 ttactctttt cccgcctctt gggatcatgt gaaagagccg gctcacgcgc aattggtata
    19081 caatttgctg aatatacctc gaaggatata ctggcgcctc ctgcgttaaa gtgtgttaca
    19141 ttccttgaat ccgttgcctc tccaggcgat tagacttata tttgacgtca gatcaaacaa
    19201 tatgagaaag ttacttttat tgctgtgcct tcctacagca ctctacgcgc aacagcctac
    19261 gaaagctgaa tatcgccaat ccaaaagtgg ttatttccag gaagtaatcc tcaccgacat
    19321 caacaaggca aaggctgagg aagcaaaaga taatcgcgtt tttaaaatcg atgcgtcatc
    19381 tgtcaatgca cctaaagacc caaccaagta tacaaccgtt tggcatacca atccggtaag
    19441 tcaggggaat accggaactt gttggtgctt ttctactact tcattctatg agagcgaagt
    19501 gaaaagaaat gcgaacattg aagtaaagct gagcgaaatg tatattgttt actggcaata
    19561 tgtggaacgg gccaaatatt ttgtagccaa ccgcggcaat atgaccttcg gcgaaggaag
    19621 tgaaacaaac ggaactacat ggatgatcaa acaatatggg atcgttcctt acgacagtta
    19681 tcacggtaaa acaagtactg ccgggtttta tgatcatgaa cctatgttta atgaaatgaa
    19741 tacctatctg caaagtatta aaacaagcaa caattggaac gaagcagaag tggtggcaac
    19801 aattaaatcg attctcaatc actacatggg cgaaccgcca acaagcgtta cttacaacgg
    19861 caaatcctat acgccgattg aatacaccaa aaatgtattg aaaattaacg ccgatgagta
    19921 tgttgatttt atgagcctga tgcaaaaacc attttatgca aaagcagaat atgatgtgcc
    19981 cgacaactgg tggaatgatg cctcatatta taacttgccg ataaatgaat tcatggagac
    20041 cttaaacact gctttgaaaa atggatatag tgtaagtatt ggtggcgatg tttcagaacc
    20101 cggatgggat agctggaata atatcgcagt agtgccaact tgggatattg catcaaataa
    20161 tatcgacgat gctgcccgcc aatatcgctt caacaccggt gctacaaccg acgatcacgc
    20221 catgcattta gttggttata ctgttcagga tggcaaaacc tggtatctct ttaaagactc
    20281 aggctccgga tcacgcaatt gcggcgccgc aagccctagc tttggatacc tttacgtgag
    20341 cgaagattat gtgaaactga aaatgatgac cttcaccgta aataaaaatg ccgtgaaagg
    20401 tgcgctggat aaatttgctg ctaagtaaat aagcaaggca tattagaaag ggcaacagga
    20461 atattgattt tctgttgccc ttttaatttt ctactacaaa ctgatagcct tttcccttca
    20521 atgttataat ttgcacacca ttagtgctgc cgaaataatc gcgaagtttt ctgatgtaaa
    20581 catcgagatt tctggaatga aaaaaactgt catcacccca cacctccaat aacaaagtct
    20641 ttctgtctat agtgcgattg atatagcgac taaaaacatc cagcacctct gcttcgcgac
    20701 tcgacaattg ccatctgttt tcgccatttt caagcaatag ctttttggga tgataaataa
    20761 atttccctat gcgaatgatt tcagatgcca cttgctgaac attgcgcaac cgtaattgat
    20821 tttgtattct gatgatcaat tcttcaacgc tgaaaggctt gcgcatgtag tctgttccac
    20881 cggattgaaa cccttctact acgtctttgg tttgtgtttt cgctgtcaga aaaataatgg
    20941 gtaaatcggg aaattgtaat cgcaggtttt tacctattgt aaatccatca acaccgggta
    21001 gcatcacatc caacacagca atatcaggtt tgaatgtcag gcatgcctgt agcaccagat
    21061 tcccatcagc aataagacgc acatcaaaac ctttttgctc cagggtctcc gaaacaatgg
    21121 tgccgaggtt ggcttcatct tcaacatata atatcttaat ggttgccatc agctctcggt
    21181 attaataaag tgaatgtgca tcctgtatcg ctattattat ttactgaaat actacctcca
    21241 gctgcttcgg tcacttgttt cacatagtgt aagccaaggc cataaccttt tacctcatga
    21301 acatttccgg tgggaatgcg gaagaatttt tcaaatatct tttgatgata cgtttcagga
    21361 atacccggac cattgtcgct gatggatatc tgcaccatat cagcaccctg tgacacatca
    21421 agtgatactt ttgtgccgcc atacttcgct gcattatcaa gcaggttttg tagcgcactt
    21481 cgtaaatgaa aggcatcacc catcacatag caaggtgttt caggtatgct aacgttgata
    21541 tcaacatgct tgctttgcaa ataattggta agtccattgg caattccggt tacatcgatc
    21601 aaggtagtgt gaaaaaactc gtgggcacca tcgccaataa cggactgcat taccttattt
    21661 acaagggtat ctaagcgtgt cacttcctct gaggccatat ttaaatagct gcgtatcttt
    21721 tccttttttt caaccggatc catttcctgc atagcctcta tggcaacttt catggtggca
    21781 acaggtgttt tcaattcatg agaaatatta ctgatcaggt cgtcttttaa ttgcgccaat
    21841 tgccattgtt tacgcaaatt ccgataagag aaaataaagg caaccaaaac taccgtgatc
    21901 aataatcccg caaaaagaaa ttgaggcacc atggtggtca ggattacact tttatatcca
    21961 tcaacctgtg ctttaaacgg ttcattaaat aaatgactat cgtactcaaa cattggtgga
    22021 ggaaaagtga atttgctcga atcatgcacc cattttattt cgagatttaa tccttggtca
    22081 gctaaatttt gttgataaat agattgaaga agtgcggtgt cttttaattc aatatgtctg
    22141 tcaaaaaatt cttttccacc cttatcgcct ttaacttcag taataaacaa tttcacacct
    22201 cgcatcaagg cattattgct gtcgatattt gtcagattag cttctgaaag agtagggcag
    22261 gtatcgcaaa atgaagtgat gcgaatcata gtatctgtgt caaatgactt tgctatgatg
    22321 gtaacggagt ctccggaggt gatagtgtcc tgagagacat gtcttatttt aaatccgcct
    22381 tcattgctca ataacggttc aataaatgag cgtgtcagta cagtatccat tacctgatcg
    22441 cgtgccgaca tgaattcctg cacgagttct ttgtgcagat ccatttcttc agaattatat
    22501 tccgtttgca gccaccttgc cgtaaacatc agcaatagca acatcgcgca gcaaaataat
    22561 gctataagcg ctcgaatgcg acgaatacct atcgaagatg ttgtcttgat tgacatcatg
    22621 caaaattagg actttcagac actttaacac ttccgcttaa ccttaattaa cctaaattgc
    22681 tttcagttaa ccccaccctt ccacacagcc ttgtagcttt gcatcaaaac aataacatgc
    22741 gaaagatcac acttttactc acaatggcat tatcaggttt gatagcccag gctcaaactt
    22801 ccggcaccat ccaatatgtt tcaacccgaa aattgcagat aaatattcaa ggtgatatgc
    22861 cacctcctcc gggattgcca agtgaaatta aactaaatac agtcttgtat tttaataccg
    22921 aagcttcact ttataaaaat gctgaaaatg caaacgatgc aatgaatgtg gagcaggagg
    22981 cagaaggcgg cggacaaatt atcatgcata tggcccctcc tgaaaatatc atgtattgtg
    23041 atctcacaaa taaaatactt acacagcaac gcgatttcat gcagcgcaaa tttttaattg
    23101 aatcaccaat agatggttca ggttggaagc tgacagggaa gcaaaaaatg gtgcttaact
    23161 atccttgcat ggaagcaatt cgcgttgata gcatagacac ggtaagtgct tggtatacac
    23221 cggtgattcc attgtcgaca ggaccacttg attatgtcgg acttcccgga atggtaatgg
    23281 aagtgcatgt gaataatgat atggaagtga ttaccgcaac tgccgtttca aaagatgtag
    23341 atgcttcaca aatcaagaaa cctaaagatg gaaagaaagt tactcaagaa gaatatgata
    23401 aaattgttga agagaaatta aaggaaatgg gcgtagaacc gggtccagga ggaagagtaa
    23461 tgattcatat tgaaaacgat taatatttta atgatgaaga aatttgcaat ttatcttgtt
    23521 tgtatctttt tgtctgttgg attgaacgca caagtgcaaa atctcagtgg gcatgttttc
    23581 aacagcgaaa gcaagcctgt tgaatatgct accgtcgcac ttttgcaacc acaggattct
    23641 acactcgctt tttttgcgat cacagatgca gaaggggcat tttctatcaa gagtgtaaag
    23701 gccggaactt acagattgca aatttctttc attggcttcg atacctacga taccttgctt
    23761 acaacgccac tgccgaatac caacttaggc gtgtatattt tgcaaacagc tgccatcgga
    23821 cttgatgcgg tacaagttac tgcggaacga attcctattc agatattagg agataccctg
    23881 cagtataatg caaactcctt taaaacggca cctgatgcct cggctgaaga attattgaaa
    23941 aaacttcccg gtgttgaagt ggatgcttcc ggaaacatta aagcccaagg tgaagacgtg
    24001 cagcaagtgc tggtagacgg taaagaattt ttcagcagcg atccgactgt ggccacaaaa
    24061 aatttaccag ctgatgccat tgaaaaagta caggtgtacg ataaaacatc cgacgatgca
    24121 gattttacag gtgtcgatga cggtcaacgc agcaaaacca tcaacctcgt gctgaaagat
    24181 ggtaaaaaaa gcatgtggct cggcaatgcg caggcaggtt atggcaccga cgatcgttat
    24241 cagacttctg ccaaattata tcgcttcacc aaaacaaatc aaatcgctgc tttaggtatg
    24301 ctcaataatg tcaatgactt tggattcagc atcaacgatt atatcgactt cagtggaggc
    24361 atagggaata tgatgcgcag cggtggattt caaatatcat cggataatga tgtccctgtt
    24421 aatttcggtc aatctgttga tggaaaaatc acaagcggag ctgcaggatt gaattatacc
    24481 tacgagcctg atgaagacca ccgcttcaat gtcagctatc tgggaaatgg ctatctgaga
    24541 aacttaacgc aaaataccag gacagaacaa ttttcagaac ttggaaattt tgaaaccatc
    24601 gactcactca atgaagaaac tgaaaaatat tttaatcgca tcaattttaa tatcagaaca
    24661 aaacctgata cacttcgctc atttattgta acaggtaatg cctccatgaa tatgggcgat
    24721 gttactagct cgggattcag cgcaaatatg tttgaggata gtcttaccaa tacactcaac
    24781 aatgcagcat attcccaaaa caacaatttc tcaggtactg ctgcggcaag ttatattcgg
    24841 aaaacaaata cgaaatggaa atactttaag gtaaaggcaa atggcagcgg aggtacatct
    24901 ttaaatgaag aaaattggaa taacctgact gcttattttg cagatggatt aacaattgcc
    24961 gacaactccg cacgcacaga tgatgc
//
LOCUS       DAOQOU010000031        24688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_954_length_24688_cov_5.473065, whole genome shotgun sequence.
ACCESSION   DAOQOU010000031 DAOQOU010000000
VERSION     DAOQOU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_24688_cov_5.473065"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            484..2118
                     /locus_tag="PLM90_04530"
     CDS             484..2118
                     /locus_tag="PLM90_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88665.1"
                     /translation="MLFRFTFITVLLCWFLTVTNAQVYPVPEITWEKCFGGSGNDSYF
                     DTEKTPDGGYISVGESTSTDYDLAGVCCPGVTKSLWLCKLDSNYNIQWQKLLQAIPTD
                     VRIDCRIFANKDQTYTLFETIDGDGVVPLCTCNHSASQDIWMFTVDNFGNIISQKCFG
                     GSAYDLMNNVSRCEDRGYIIGASSGSGDGDVGIHYGSPFTTDAFIVKTDSLGNIQWTK
                     TIGGTGWEGAFVSGISGNRCLINIGASSNDYDLAGKMPLGETMVNWLMITDSAGNILQ
                     DTVVRAYDGKLDLNFPYEYKKNNYLIVGYISMDTGMYAMAYGGGDALIAVFDSSLALE
                     KVVQFGGTKLDNIYKMNKLNDSTIYFTGRTYSSDYDAQECRGLWDAFIVKTDTSLNKL
                     WSRSIGGSSADVFSNLFQSDSTIVLFGSSAINSADDGDILNAHYDEEIGLSSDSWVIF
                     LNENVPYDTVYLIPEIKIYPNPSSNQVNIVISADVDMDYNLAIYSLDGKMALSESLSS
                     NIVNEVKVNSLPSGYYIFSIRDGSKNEIVSKKILIL"
     gene            complement(2273..3880)
                     /locus_tag="PLM90_04535"
     CDS             complement(2273..3880)
                     /locus_tag="PLM90_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ88666.1"
                     /translation="MKKLITLLLMTALVSIDQRLTAAPLLCSADFTVTISGATASFNG
                     SGTGITPFYSWDFGDGSYGSGEDISHTYTFNGTYIVCLTVWGADSCMAMTCDSVVITG
                     AAAGGSCHADFTYFIAGTDVSFDNNSDAGGAIDADFFWSFGDGGTSTAVNPEHSFAPG
                     TYEVCLDIFTSDSCSDTYCTVIAVGGIIDSLDSLECNAYFTYDFLMGEVNFTNLSDDG
                     GAAFSSYFWDFGDGTTSTEENPSTTFDPGWHTVCLTINTSDSCSSTYCTDIYIWDGGG
                     DTTVCEAGFDFTVTGMSADFTNTSSGGGAFITSYNWTFGDGGTSTATNPEHAYAAEGI
                     YNVCLTIYTTDSCSSTYCETVVISTGDTTGCTALFTYDFGITPWGIFTTNLSDDGGTG
                     DASYFWDFGDGTSSTEEEPAHNYAGGGDYLICLTISTDSCEDTYCSSVSIAPTPIAET
                     ELFEGLTLYPNPARDVINIHVALHADVTCILTDLSGRELSVRTLQANAGNTEHQINIA
                     DLESGIYTILLIDAAGNRAMEKIMVVR"
     gene            complement(4001..5212)
                     /gene="porV"
                     /locus_tag="PLM90_04540"
     CDS             complement(4001..5212)
                     /gene="porV"
                     /locus_tag="PLM90_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HMZ88667.1"
                     /translation="MQSTLKITTIAVVLTMISTTTRAQISLGNGTGDDYLNTVTTAVP
                     FLRIAPDARSGGMADVGLAISPDANSIFWNPAKTVFIDNEHPYGMSLTYTPWLSNLVN
                     DIYLAYLNGYWKVDELSAVSASLRYFSLGTITFTDASGNVLQDFRPNEFAFDVAYSRK
                     LADNLSAGLDLKYLYSNLATDQVVNGVQIKPARGVAADISMFYHTKFDTGDKDSELGV
                     GLNISNIGNKITYTSSIEKDFIPTNLGLGAAYTVNFDSYNALTFAVDMNKLLVPTPDS
                     TDADNDGILDFKEESTVSGMLGSFSDAPNGASEEFHEIMWSTGLEYWYAERFAVRTGY
                     FYEHATKGGRQYFTVGLGLRYSVFGLDFSYLVPTSNNQNPLDNTLRFTLIFNLENLKG
                     GGSSEDSGSDS"
     gene            complement(5273..9175)
                     /gene="porU"
                     /locus_tag="PLM90_04545"
     CDS             complement(5273..9175)
                     /gene="porU"
                     /locus_tag="PLM90_04545"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HMZ88668.1"
                     /translation="MKLTTSRYRLTAAMLTCLIVNAVSAVAQISVDRTLNWSATPRSF
                     SGEGVNEVFSLQFTGASYTTEGEVILPLYAESFPASGNKIVSAKINNAQYAPIETANA
                     KIAAGMRTKISNEIKINAGIGYARKKPEANISFIPLRINPSTGNIEKLVSFTLDIVTA
                     NDQQLKITGEDDFTGNSVLSSGSWYKFAIKREGIFKVDRAVLSSMGISGTVSFSTFGV
                     FGNGGGMLPESNLTARYDDLQENAIYKYDANGNGMFEDGDYILFYGQAPHRWYYNTVT
                     NTFKHKNNIYSDQTCYFITTDRGNAKYITAQASVAGENYNTSSYDIMSFIDQDKVNLL
                     NSGRAWYGDIIDIYTPNRTYNFTLPDLITSEYILFRASAAGNSTSGSITIDLKANGAS
                     LPSDITISEVSEGFSETYAYENGVETGFNSTSSSIAISMDYYGSSVATAWVNYIELIG
                     RANIVYNNTPLFFRDARTIGDGRITKFTIDAPNGIQIWDITDRINAKLQNYTVSGSDL
                     TFTLATDTLKEFVAVDPNDAYGADDITFIEKVVAQDLHNDANQPDLVIVSHPSFMSEA
                     RRLAGWHHDVDGLDTLVVDVNKVYNEFSSGVQDISAIRDMMRMFYERAGGDESLMPQY
                     LLLFGDASFDYRFLAFDEEKNTLKIPCFESNESLARAVSYGTDDYYGFLDPTEGDNIE
                     DINEKLDVGIGRLPVMTVDEATIMVDKIMHYKSPESLGSWRNALTFLADDEDYNTHVE
                     DAEELAEYMTTNYPVYNVDKIFFDSYQQIPGAGGERYPEVETAIDNKIFTGSFIMNYL
                     GHGNEQNWAQERVLSVDDINSWDNYDKLPLFITATCSFSRYDNPDLRSAGELVLLNPQ
                     GGGIALVTTVRLVIASANYDLNSNLIEHLFEPVDGEYPRLGDAVRLGKNGVTASAQNN
                     RKFILLGDPALQLNYPHQRVVTKTVNGFDVAVYTDTLKALEKVTITGEVTDESGVKLT
                     GFNGVVYPAIYDKPILINNLVNDPTFSGVGGYGPSEPFSFYLQKNALYKGKASVVNGE
                     FTFTFIVPKDISYTYGNGKLSYYADNGTDDANGYNTNVIIGGSSDDAAADNVGPDVDV
                     FMNDESFVFGGLTDENPVLLIKLIDSSGINALGNAIGHDITATLDDDQQTMLKLNEYY
                     EADLDKYQSGSVLYPLNDIAEGRHTVTVKAWDVYNNSGDGYTEFVVAETADLALDHVL
                     NYPNPFTDNTSFWFEHNRPGDVLDVKIEIFTVSGKRVKTIQQQVITDGYRVDDITWDG
                     LDDYGDVIGKGVYVYKLSVKAASDNSKASEFQKLVILK"
     gene            9201..10271
                     /locus_tag="PLM90_04550"
     CDS             9201..10271
                     /locus_tag="PLM90_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ88669.1"
                     /translation="MKVFLERKYQKNIATSRSFKGQYSLLCITGLLLISFLSAQKLSA
                     QDLRYSQWHAAQTTVNPAFAGAFGRPRIILNFRDQWPMMPQSYVSYRAAMDAYVPKIR
                     SGLGVTIDQDNQGDNLLQSTRFGMQYMYQARLGAEWALNFGLSLDYRQYRINWQDIQF
                     YDQISLLTGFNDVSGNPNPTAEPAPGKNQVGFVDLGSGLLLYNKSAYLGFSFNHITNP
                     VVSFYGTDNSELPGSLNAQAGVFIGGKREKDLLVNPYALWSHQAGFNQIQTGVYVKKS
                     LLLTGLAVKHNTVGLSDVVLLAGVSQETIRFAYSYDVSTGPLAGLTGGAHEVSLVITL
                     KENAGRAFRNSQKSMLDCPSIL"
     gene            10325..11848
                     /locus_tag="PLM90_04555"
     CDS             10325..11848
                     /locus_tag="PLM90_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ88670.1"
                     /translation="MKRNLHPVLVLSMTVLVLAFTTSCKKRSAVSGFKYNDSKWGGFE
                     AHRFDGQATGPNLVLVQGGTVVQGTTEQDVTFEYNNIPRRVTVSSFYMDETEISNLHY
                     REYVYWVTKQFPDFPQVIKNSLPDTLVWREELAFNEPYVQYYFRHPSYQNYPVVGVSW
                     LQATEFCKWRSDRVNEMILAEKKYIDVNTMGTGEDNFTGETYMTGQYTTGLRKGKKDY
                     SPYAADPKMGRTVRMEDGILLPDYRLPTEAEWEYAALGLVGNRVPGNEMITDRRLYPW
                     DGNTLREPSAREKWAQGKMLANYKRGRGDYAGIAGKLNDNSIITAPVYSFMPNDYGLY
                     NMAGNVNEWVADVYRPTTYGEMEDLNPYRGNEYQVRDLDEDNFPVIDTLGRIRYRPVK
                     DEEAVNRRNYKKGYVVDFLDGDSTSAVTYDYGRSTLISDNARVYKGGSWADEAFWCSP
                     GSRRYLEEDLSTATIGFRCCMIRLGGDVDNETKGGNNFKKTKKQQKLNKTNRNMNKF"
     gene            11911..13182
                     /gene="murF"
                     /locus_tag="PLM90_04560"
     CDS             11911..13182
                     /gene="murF"
                     /locus_tag="PLM90_04560"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMZ88671.1"
                     /translation="MIAELYSRFSHAKGVTIDNRTISGGEMFFGLRGEKVNGGMFAAE
                     ALKNGASCCVVDDPHFATDANIILVPDALVAMQELAAHYRSTWQFPVLALTGSNGKTT
                     TKELIAAVLSKKYRTYATKGNLNNHIGVPLTILNIGADCEFAVIEMGANHQGEIASYC
                     KYADPDFGLITNIGLAHLEGFGGIEGVAKGKSELYQYIIKKDGVIFWNSNQERLTDIA
                     KTYARTVRYGTNPEDYTIGIIDETATMATVVIDDEIKISSHLVGAYNADNIIAAACIG
                     KYFDVSYGEIQEAVENYIPANNRSEHREINGNYFILDAYNANPSSMRVALENFAKISA
                     QKKVIIIGDMLELGTYSADEHKQILDIALHSKFDHIVTVGPQFHHIRRDPVIACHNSD
                     EARQWFLAQQFREATILLKGSRGMKLEKIIQ"
     assembly_gap    13388..13487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13708..14868)
                     /locus_tag="PLM90_04565"
     CDS             complement(13708..14868)
                     /locus_tag="PLM90_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HMZ88672.1"
                     /translation="MITKLLTPVRISDFLQDWTIQPYHWARHIRMYTNEFPDTNNCLI
                     ALIGVNESRGSMQNSGCNTGADTVRRHLYQLAISEYPLQMIDAGDVIIGETPDETYQN
                     VKMVMEWFLGQQILPILIGGSQDITIGQYAGYEFLGQAVNLMVIDERVDLDDTELLPP
                     GRTWLQQIFRYDPKFLFNYSAIAYQTHFNSPEMLGAIDDLLFDTIRLGRLKENIQFAE
                     PEVRDADILSIDIGAIRGADAPGIAHATPNGLSGWEICQIMRYAGMSDKITSLGIYEY
                     NPMFDNQELTAKLVAQMIWYFVDGFYNRKSDYPIVSEKDFTKYIVNVAESDYDLTFWK
                     SMKSGRWWVEVPFIVQQRFERHQLVPCSYEDYENSLKNEVPEKWLRIFHKLS"
     gene            15030..16115
                     /locus_tag="PLM90_04570"
     CDS             15030..16115
                     /locus_tag="PLM90_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMZ88673.1"
                     /translation="MQDFLNHEFLHNTWRQWCWLIGVIIFVVIFKRYISKLISMLLFK
                     IVQRTAAGELSQEFLKRILKPLQYFIVLQTIYIGFHTLNYPFNKENEEGMALVQTILF
                     GAYRFLMILNIAWIVSRIGDFFVMVLKHRAEKTEDPWDDQIVVFLKDIIRLLVWTIAI
                     LSILGAVFGVNIYSIIAGAGIAGIAIAFAAQESLQNIFGSIAIFTEKPFVIGDLIDFD
                     GTQGTVVKVGFRSTRLRTIDTAYMTVPNKTIVNSKLSNLTRRTSRRVYFTIGLSHDTS
                     VEQLEAIRKEIYAFGMQHPKRNDEVNIGVTQYNTAAIEMLVEIHFEYVNWSAFVELRN
                     ELMLAIIQIVKKHGAVFAQQLAIAEKK"
     gene            complement(16177..17403)
                     /locus_tag="PLM90_04575"
     CDS             complement(16177..17403)
                     /locus_tag="PLM90_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HMZ88674.1"
                     /translation="MKKSTTLIMSMVCTVVTVRAQLTPEITSWITDAGSSNFHDIIYS
                     NVQQVQYTDADVFISCDAIAAYDVGPWDDPALGSNQNFVFRIPRLPQAADPTPLQPGH
                     IGVWSNGLSIFDIRSGQSYHNNNIWHVDAVAENAEVEPSCTGRIAENGEYYNYTAPAC
                     LFDPTQNTEHATILGYAFDGYPIYGAYAFENTDGSGNIVRMRSSYQLRNITLRNSLPD
                     GTMLSAEDQGPDVSNNYPLGRYLEDYIYVAGSGDLDEHNGRFCITPEYPQGTYAYFTT
                     INESGKPAFPYVIGGSYYGRVAEGNTGTFSGHQVIDESAATYTSVGADAAVLDMLVYP
                     NPTTDYIHVYIVPSYANDITATVRDISGRMVYQQNNMQPGVSYPIDARGLNSGLYFIE
                     LKSSDAYGMQKCMVRH"
     gene            17664..18764
                     /gene="carA"
                     /locus_tag="PLM90_04580"
     CDS             17664..18764
                     /gene="carA"
                     /locus_tag="PLM90_04580"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMZ88675.1"
                     /translation="MENFEKVPAVLLLADGTAFYGFSAGKIGTTTGEICFNTGMTGYQ
                     EIFTDPSYFGQILVMTNAHIGNYGTYSEDVESENIKISGLAVKNFTNQFSRRMTTTTI
                     QDYLLDEHLVAISDVDTRAIVRHIRSKGAMNCIISSETTDIEVLKTKLAETPDMNGLE
                     LASIVSTKTPYFIGNSTAEKKVLVYDFGVKKSILQNIADRGCYLKIVPANTPFEETTS
                     FDANGYFISNGPGDPASMGYAVDTVKKILAADKPLFGICLGQQLLALANGVSTYKMHH
                     GHRGCNHPVKNLETGLCEITTQNHGFSVREDELRANNNLEITHMNLNDQTVEGFRLKN
                     KPAFSVQYHPEATPGPHDARYLFDQFVKSMHV"
     gene            18792..20075
                     /gene="eno"
                     /locus_tag="PLM90_04585"
     CDS             18792..20075
                     /gene="eno"
                     /locus_tag="PLM90_04585"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMZ88676.1"
                     /translation="MSNIIHIHARQILDSRGNPTVEAEVLTEFGAIGRAAVPSGASTG
                     IHEAVELRDEDPDVYLGKGVLKAVEHINESIVHELEGVDIFNQNLIDQIMIELDGTPN
                     KSKLGANAMLAVSLACAKAAASELSLPLYRYVGGVNANTLPVPLMNILNGGAHADNKI
                     DFQEFMIAPIGGDNFSDALRMGVEVFHHLKKILKNKGYSTNVGDEGGYAPNIQTNEEA
                     IETVLMAIESAGYVPGEDITIAMDAATSEFYNAEKGLYIFKKSDGRQMTPAEMVAYWA
                     EWAKKYPITSIEDGCAEDDWEGWKLMTQQLGNKIQLVGDDLFVTNVKRLSKGIKEHVG
                     NSILVKVNQIGTLTETIQAVDMAHRARYTSVMSHRSGETEDTTIADLAVALNCGQIKT
                     GSASRSDRIAKYNQLLRIEEDLGSMAVWPGNVFLR"
     gene            20195..20521
                     /locus_tag="PLM90_04590"
     CDS             20195..20521
                     /locus_tag="PLM90_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HMZ88677.1"
                     /translation="MTTKRSGFRQRLRQWKVPALLVNKYVFTLFVFGVWMTFFDQNNF
                     MRQYRRIAELNAVKSKTAYYESETAKAKKQLQALQNDQKALERFAREKYYMKKPNEDV
                     FVIIDK"
     gene            complement(20554..22650)
                     /locus_tag="PLM90_04595"
     CDS             complement(20554..22650)
                     /locus_tag="PLM90_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88678.1"
                     /translation="MQLQIFSDYPWWFWFLCVLGGALYAAALYWRERRIAEVLPKQWW
                     IPAALRFIGATILLMLLLNPYMRSSTNETEKPIVVLLQDNSSSIAKGIGDTTAFLDAW
                     ESMATELSKDFDVRKYVFGETMEEDGENHFDQASTNISQSLLSLNNLYEQQHVAGVIL
                     ASDGIYNSGSNPVFIKNELNAPIYTVALGDTTRKKDVRILRTLHNNLVYLDDRFTVQA
                     DIEGIFCNGINISASISEINKNGNRNLGEQSVNAVGDQFNGSVKWEIAAQEPGIRHYR
                     INVRKVEGEFTTENNVQDIYIEVLDARQKILLLANAPHPDLQALRLSITQNKNYDVDV
                     QLAGEWKGNIADYDLVILHQLPSSTNTIPQVINEVQAKRIPTWFITGSQTSVVAFNKA
                     QSLLNINSNGQSANEVTALMDLQFNLFTIDDKTLGILPRLPALSAPYGQYTASKASQV
                     VAYQKIGSVATKTPLLLFSIPGGEKTAVLCGENIWKWRLYDYVLNNTQEASDEIIGKT
                     VQYLAAKNDKKQFRVSQARAVYTEQENIILDAELYNDTYELINTPDATITITDESGKD
                     FDFQFSKTGNSYTLSAGYFSPGNYSYKASTTFNGKTYTDAGAFSVSPVRLETINITAD
                     HRLMNQLAVQTGGSMAYPENMASLVDAIKNSAQAKPVMREITRTQSIIHLKWIFAVIL
                     LLLSVEWFIRKYSGGY"
     gene            complement(22764..23510)
                     /gene="fabG"
                     /locus_tag="PLM90_04600"
     CDS             complement(22764..23510)
                     /gene="fabG"
                     /locus_tag="PLM90_04600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HMZ88679.1"
                     /translation="MGLLTNTTVLITGASRGIGEAMAMRFAEAGANVAFTYLSSEARA
                     QALEQKLSEMGVKAKGYKSNAGVYAEAEKLINDVIADFGNIDTLVNNAGITKDTLMLR
                     MSEEDWDAVMDVNLKSVFNLTKFAIKPMMKARKGSIINISSIVGLIGQAGQANYSASK
                     AGIIGFTKSIAKELGSRNIRCNAIAPGFIETDMTGELSEELKQTYFKSIPLGRFGQGK
                     EVADVAIFLASEMSSYVTGQVISVCGALHT"
     gene            complement(23767..>24688)
                     /gene="sucD"
                     /locus_tag="PLM90_04605"
     CDS             complement(23767..>24688)
                     /gene="sucD"
                     /locus_tag="PLM90_04605"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMZ88680.1"
                     /translation="TIGETILKSSYLRGMSVLVGKNTRLVVQGFTGKEGTFHAEQMIA
                     YGTNVIGGVTPDKGGTKHLDRPVFNTVEDSVKKEGANTSIIFVPPAFAADAIMESADA
                     GIQVIVAITEGIPVQDMVRVKAYISNKSCTLIGPNCPGVITPEEAKVGIMPGFIHKKG
                     SIGIVSRSGTLTYEAVDQVTKAGMGQSTCIGIGGDPVPGTTTLDAIKLLMADPETEGI
                     ILIGEIGGTMEANAANWIKEHGTKPVVGFIAGQTAPKGRTMGHAGAIVGGKDDTAAAK
                     MEIMRNCGIHVVESPAVLGETMAMAMKGRI"
BASE COUNT         7038 a         5101 c         5313 g         7136 t
ORIGIN      
        1 gcgcattttg tcacttcagg gcaggccctg gggaggggtg tagtggtctg ccttaggcag
       61 accgttttgt actaaaaatc aagcctgccc cgaagtgtaa atttttgtag cgcagagaag
      121 aaattttcta cttccggggc taatgaagaa tctccatttc gagaagaaca taaacccaac
      181 cctgaattag aaaaatgctc cgctccgcta cgcattttgt cacttcaggg caggccctga
      241 gggagggaat agggaggtct gccgaaggca gatctttgtt gttatggata taaatttcaa
      301 gccaatcctg ttgaaactta ttgttgcagg ttaggaggat tgggtaggtt taaagttcta
      361 aacctgtgtt tgaaagatac ggttcgatgt aggtttagta aatttagggt tgttttattg
      421 taaccactga ttagaagatt gctgcaaaat gccatttgtt taaaaataca tatccaatat
      481 taaatgctat ttcgctttac atttattact gttttactat gttggttcct tacggttacg
      541 aacgctcagg tttatcctgt tcctgaaatt acatgggaaa agtgttttgg aggttcaggg
      601 aatgacagtt attttgacac tgaaaaaaca cctgatggag gctacatctc agtaggtgaa
      661 agcacttcca ctgattatga tcttgcaggt gtttgttgcc cgggtgtaac aaaaagttta
      721 tggctctgta aattagacag taattacaac atccaatggc aaaaactact tcaggcaatt
      781 cctacagatg tcaggatcga ttgccgtata tttgcgaata aagaccaaac ttacacactt
      841 tttgaaacaa tagacggtga tggtgtcgtt ccgctctgca cctgtaatca cagtgcgtcg
      901 caggatatat ggatgtttac tgttgacaat tttggaaata ttatcagtca aaaatgtttt
      961 ggtggtagtg cctatgatct gatgaataat gtatcaagat gtgaagatcg tggttacata
     1021 attggagcct catcgggttc aggtgatggt gatgtaggca tacattatgg gtcaccattt
     1081 accactgatg cctttattgt caaaacagat tctcttggta atatacaatg gacaaaaacg
     1141 attggtggta caggatggga aggtgctttt gtttctggta tttcaggtaa tagatgttta
     1201 attaacattg gtgcaagttc aaatgattat gatctggctg gcaaaatgcc tttaggtgaa
     1261 actatggtga attggttaat gattacagat tctgccggaa atatattgca agacactgta
     1321 gtaagggcct acgacgggaa actcgacctt aattttccct acgaatataa aaaaaataac
     1381 tatttaattg ttggttatat ttcaatggat acaggcatgt atgcaatggc atatggtggt
     1441 ggagatgcat tgatagctgt ttttgattca tctcttgctc ttgaaaaagt cgttcagttt
     1501 ggcggaacga aattggacaa catttacaaa atgaataaat taaacgactc gacaatatat
     1561 tttacaggca gaacttattc atcggattat gatgcacagg agtgtcgtgg cttatgggat
     1621 gcatttattg taaaaacaga cacctcgctt aataaattgt ggtctcggag tattggaggt
     1681 agtagcgctg atgttttttc taatctattt caaagtgatt caactatagt tctttttggc
     1741 agtagtgcaa ttaatagtgc agatgatggt gatattttga atgcgcatta tgacgaagaa
     1801 atcggccttt caagcgattc ctgggtcata tttttaaacg aaaatgtacc atatgacacg
     1861 gtatacttga tccctgaaat taaaatttat cccaacccat caagtaatca agtaaatatc
     1921 gtgatcagcg ctgatgttga catggattat aatttggcta tttattcttt agatggaaaa
     1981 atggcactaa gtgaaagttt gtcttcaaat attgtaaacg aagtcaaggt aaattcgctt
     2041 ccttccgggt attacatttt ctcaattcga gacggatcga aaaatgagat cgtatcaaaa
     2101 aaaatattga ttttatagtt tattggttat agaagcggag acaattttct acttccgggg
     2161 ctaatgaaga attctaattt tatgatgatc aagatatttc atcgatattg aaatcaaaaa
     2221 caaaaaaccc gaaacgcaat cgcttcgggt tttcttgtca gcagaattaa actcaacgca
     2281 ctaccattat tttttccatg gccctgttac cggctgcatc gatcaacagg atggtgtata
     2341 taccgctttc aagatcggcg atattgatct gatgttctgt atttcctgca ttcgcttgta
     2401 aggtgcggac acttaattcg cgcccactca agtctgtgag aatacaagtc acatcggcat
     2461 gcaatgctac gtggatattg atcacatctc ttgccggatt tggatacaag gtaagtcctt
     2521 caaataattc tgtttctgct atcggcgttg gagcaatgct tacactgctg caataggtgt
     2581 cttcacaact atcggtagaa attgtaaggc aaatcaggta atcgccaccg cctgcataat
     2641 tgtgtgccgg ctcttcttct gttgagcttg tgccatcacc aaaatcccag aaataacttg
     2701 catcgcctgt accgccatca tctgaaagat tagtggtgaa aataccccaa ggagtgattc
     2761 cgaaatcgta agtgaataag gctgtgcatc ctgtggtatc gccggtagat attactacag
     2821 tttcacaata ggtgctggag catgaatcag ttgtataaat ggtcagacac acattgtaaa
     2881 tgccttcagc agcatatgca tgttcagggt tggtggcagt ggatgtaccg ccatcaccaa
     2941 aggtccaatt gtatgatgtg ataaatgccc cgccacctga tgaagtattc gtgaaatcag
     3001 cactcatacc tgtcaccgtg aaatcgaaac cggcttcgca aacagtggtg tcgccaccgc
     3061 cgtcccagat atagatatct gtgcaataag tactgctgca gctgtcgctt gtattaatgg
     3121 tcaagcatac agtatgccat cccggatcga atgtggtgct tggattttct tcggtagatg
     3181 tagtgccatc accaaaatcc cagaagtaag aggagaatgc tgcaccgccg tcatctgata
     3241 ggttagtaaa attcacttca cccattaaaa aatcgtacgt gaaataggca ttgcactcga
     3301 gtgaatctaa tgaatcaata ataccaccta cagctatcac ggtgcaataa gtatcactac
     3361 aactatctga agtgaaaata tcgagacata cttcataagt gcccggagca aaactgtgtt
     3421 cgggatttac tgcggtagaa gttccgccat cgccaaagct ccaaaagaaa tcggcatcga
     3481 tagcaccacc ggcatctgaa ttattatcga agctcacatc tgttccggca ataaagtagg
     3541 tgaagtcggc atggcaactt ccgccagctg cagcacctgt gatcacgaca ctatcgcatg
     3601 tcatggccat gcaactgtcg gcaccccaaa cggtcaggca gacgatatag gtgccattaa
     3661 atgtgtaggt atggctaatg tcttcgcctg aaccatagct gccatcgcca aagtcccagc
     3721 tataaaatgg ggtgatgccc gttcctgagc cattaaatga tgcggtagca ccggaaatag
     3781 ttaccgtaaa atcggcagag catagcagag gagcagctgt gagccgttgg tctattgaaa
     3841 ccagtgcagt catgaggagg agtgtgatca attttttcat atcattcaaa ttttattgta
     3901 acaacatcct acacgacact caggataaaa tggttgggtt agacaccaaa aaaaaagagg
     3961 cgaacatgtc gcctctttta taatatgttg ccggtcgtta ctaactgtcg gaacctgaat
     4021 cttcgctgct accgccgcct ttcaagtttt ctaagttgaa aatgagggtg aaacgaagtg
     4081 tattatccaa tggattttga ttgttgctgg taggcaccag atagctgaaa tcaagtccga
     4141 atacgctata acgcaatcca agacctacag taaagtattg gcgaccacct tttgtggcat
     4201 gctcatagaa atagccggtg cgcacagcaa aacgctctgc ataccagtat tcaagacctg
     4261 tgctccacat aatctcatgg aattcttcgc tggcgccgtt tggtgcatca gagaatgaac
     4321 cgagcatacc gctcacagtg ctttcttctt tgaaatcgag gataccgtca ttgtcggcat
     4381 ctgtagaatc cggtgtaggt accaataatt tgttcatatc aacagcaaat gtcaacgcat
     4441 tgtagctgtc gaaattcacg gtataagccg ctcccagtcc aaggttggtt gggataaagt
     4501 ctttttcgat agagctggta taggtaatct tatttccgat attggagata ttcaatccca
     4561 cacccaattc agagtcttta tctcctgtat cgaatttggt atggtagaac atgctgatat
     4621 cagcagccac accgcgagca ggtttaattt gcacaccatt caccacctgg tcggtagcga
     4681 ggtttgaata aaggtatttc aagtcaagac ctgcagataa attatcggca agcttgcgtg
     4741 agtaggctac gtcgaaagca aattcgtttg gacggaaatc ctgcaataca ttgccggaag
     4801 catcggtaaa tgtgatggtt cccaaagaaa aatagcgcaa tgaggcactt acagccgata
     4861 attcatccac tttccaataa ccgttgaggt aagcaaggta aatgtcattt acgaggttgc
     4921 ttaaccatgg tgtataggta agcgacatac cgtatgggtg ctcattgtcg ataaaaacgg
     4981 ttttagcagg gttccagaag atagagtttg catcagggga aattgccaaa ccaacgtcag
     5041 ccatcccgcc ggaacgcgca tcaggagcga ttctcaggaa gggtactgcc gttgttacgg
     5101 tattcaaata gtcgtcgccg gtgccgtttc ccagggatat ctgagcacga gtggtggtgc
     5161 tgatcatcgt caaaaccacg gcgattgttg taatctttaa tgttgactgc atattatgtt
     5221 tgataaaatc tagggttcgc aaaaatacaa tattctgtaa cgtatcgggg cactacttta
     5281 gtataacaag cttttgaaat tctgaagcct tagaattgtc gctggcggct ttaacactca
     5341 gtttgtagac ataaaccccc tttccgatca catctccgta gtcatccagc ccgtcccaag
     5401 tgatatcatc taccctgtat ccgtcggtta ttacctgctg ctgtatggtt tttacgcgct
     5461 taccactcac agtgaaaatc tcgattttaa catccagaac atctccgggc ctattgtgtt
     5521 caaaccaaaa actggtattg tcggtgaaag gattcggata gttcaaaaca tggtcaagcg
     5581 caaggtcggc agtttcggct accacaaatt ctgtataacc atcgccactg ttattgtaaa
     5641 cgtcccaggc ttttacggta accgtgtgtc ggccttcggc gatgtcgtta agcggataga
     5701 gtacagaacc actctggtat ttgtcaagat cagcctcata atattcgttg agtttgagca
     5761 tggtctgctg atcatcatca agtgtagctg taatatcatg accaatggca tttccaagcg
     5821 cattaatacc gctgctgtcg atgagcttga ttaacaaaac cggattttca tctgtcaatc
     5881 cgccaaaaac aaatgattca tcattcatga acacatctac atcaggaccg acattatctg
     5941 ccgcagcatc atctgatgaa cctccaataa tcacatttgt attatatccg ttggcatcgt
     6001 cggtgccatt gtcggcataa taactgagct tgccatttcc gtaagtataa gaaatatcct
     6061 taggcacaat aaatgtaaag gtgaactcgc cattcacaac acttgcttta cccttataca
     6121 aggcattttt ttgcaaatag aaactaaatg gctcgctagg cccgtatcca ccaacgccgc
     6181 tgaatgttgg atcattaaca agattattta tcaggatggg cttatcataa attgccggat
     6241 aaacaacgcc gttaaaaccc gtcaatttaa ctccgctttc atctgtcact tctccggtga
     6301 tggttacttt ttcgagtgct ttcaaagtat ctgtgtatac ggcaacatca aatccattca
     6361 cggttttcgt taccacgcgt tgatgcggat aatttaattg taatgcagga tcaccaagca
     6421 aaatgaattt gcgattgttt tgggcagatg ccgtaacacc atttttccct aatcgcacag
     6481 cgtcacccaa acgcggatat tccccgtcga caggttcaaa caagtgttca attaaattac
     6541 tgttgaggtc ataatttgct gaggcaataa ccagtcgtac ggtagtaacc aatgcaatgc
     6601 cgccaccttg cggatttaat aacaccaatt cacctgcaga gcgtaaatca ggattgtcgt
     6661 atcgcgaaaa tgagcatgtt gcagtaataa ataatggcag cttgtcgtaa ttatcccaac
     6721 tgttgatgtc gtcaacagac agcacgcgct cctgcgccca gttttgttca ttaccatgac
     6781 cgagataatt cataataaat gaacctgtga aaattttatt gtcaatagct gtttctactt
     6841 ccggataacg ctcaccaccc gctccgggaa tttgctgata cgaatcgaaa aatattttgt
     6901 cgacattata caccggataa tttgtggtca tgtattccgc caattcttct gcatcttcaa
     6961 catgggtgtt gtagtcttca tcatctgcca aaaatgtcaa ggcatttctc cagcttccta
     7021 aagattccgg cgatttataa tgcatgattt tatcgaccat tattgtggct tcatcaactg
     7081 tcattacagg taatctgcca atgcctacat ccagtttttc attaatgtct tctatattgt
     7141 cgccttctgt aggatctaaa aatccatagt agtcgtcagt cccgtaagat actgctctgg
     7201 caagcgattc attgctttcg aagcatggaa ttttgagtgt gttcttttct tcatcgaagg
     7261 caagaaaacg ataatcgaaa gaggcatcgc caaacaataa taaatattga ggcatcagtg
     7321 attcatctcc tcctgcacgt tcataaaaca tgcgcatcat gtcgcggatg gcagaaatat
     7381 cctgcacgcc ggaactaaat tcattgtata ctttattgac atcaaccacc aatgtatcca
     7441 atccgtccac atcgtgatgc catcctgcca atcgacgtgc ttcactcata aaagaagggt
     7501 gacttacaat tacgagatcg ggttgattag catcattgtg caggtcctgt gcaacaactt
     7561 tttctatgaa ggtaatgtca tcggctccat atgcatcatt cggatcaaca gcaacaaatt
     7621 cttttaacgt gtcggttgcc agtgtgaagg tgaggtcact tccagaaaca gtataattct
     7681 gtaatttggc attgatgcga tcggtgatat cccaaatttg aataccattc ggtgcatcga
     7741 tagtgaattt tgtgattctg ccatcgccga tagtgcgcgc atctctgaaa aataatggag
     7801 tgttgttgta aacaatattg gcgcgaccaa ttaattcgat ataattcacc caggcagtag
     7861 ctacagaaga accataatag tccatgctga tggcaattga agaagatgtt gaattaaagc
     7921 ctgtttcaac accattttca taagcatagg tttcgctaaa tccttctgaa acttcagata
     7981 tggtaatgtc tgatggaagg cttgcgccat tggctttcag gtcgatggtg atggagcctg
     8041 aagtgctgtt tcctgcagcg cttgcgcgga ataaaatgta ttctgatgta attaaatcag
     8101 gcagggtgaa attatacgtg cgattaggtg tatagatgtc aatgatatcg ccataccacg
     8161 cgcgaccgga gttgagtaaa ttcactttat cctgatcgat aaaactcatg atatcatagg
     8221 atgaagtatt gtagttttca cctgcaacag aagcttgtgc agtaatatat tttgcattgc
     8281 cgcgatcagt ggtgataaaa taacaggtct gatcggaata aatgttgttt ttatgcttga
     8341 aggtgtttgt tacggtatta taataccatc gatgtggtgc ctgaccataa aataaaatat
     8401 aatctccatc ctcaaacatt ccattgccat tggcatcata tttataaatg gcattttcct
     8461 gcaaatcgtc atagcgtgca gtgagattgc tttccggtaa catgccgcct ccgttgccaa
     8521 aaacaccgaa ggtagaaaaa gacacagttc cacttatgcc catactactt aatacagcgc
     8581 gatcaacctt gaaaataccc tcgcgtttta tagcgaattt ataccaactg ccacttgata
     8641 ataccgagtt tccggtgaaa tcatcttctc ccgtaatttt taattgttga tcattagctg
     8701 ttacaatatc aagcgtaaat gaaacgagtt tttcaatatt acccgttgaa ggatttatgc
     8761 gcaaggggat aaaactgata ttcgcttcag gtttttttct cgcataaccg attcctgcat
     8821 ttattttaat ttcattgctg atttttgtcc gcatgcctgc agcaattttg gcatttgctg
     8881 tttcgatagg agcatattgt gcattattta ttttggcaga aacaatttta tttccggagg
     8941 caggaaatga ttcggcatat agcggtagaa ttacctcgcc ttcggtagtg tatgaggcac
     9001 cggtaaattg caaactgaat acttcattta ctccctcccc ggaaaatgat ctgggcgttg
     9061 ctgaccagtt gagtgtgcga tccactgaaa tttgtgcaac agcagatact gcattcacaa
     9121 tcaaacacgt taacattgca gccgttagcc tgtagcgtga tgtcgtcaac ttcatgaact
     9181 tcaaaatcag gtggttttag gtgaaagtgt ttcttgaacg caaatatcaa aagaatattg
     9241 ccacttcccg gtcatttaag ggtcaatact ccctattgtg tataacaggt cttttgctga
     9301 taagtttcct cagcgctcaa aaactatctg ctcaagatct gaggtacagt cagtggcatg
     9361 ccgcccagac cacagtaaat ccggcatttg caggagcctt tggccgcccg cgtatcatcc
     9421 tcaatttccg tgatcaatgg ccgatgatgc cccaatctta tgtttcatat cgcgccgcca
     9481 tggacgctta cgtgcctaaa atccgaagcg gactgggtgt taccatagat caggataacc
     9541 aaggcgacaa tctgttgcaa tctacccgct tcggcatgca atatatgtat caggcgcgat
     9601 tgggagctga atgggcattg aattttggtc tcagtttaga ctacaggcag tacagaataa
     9661 actggcagga tattcaattc tatgatcaaa tcagtttact caccggtttt aatgatgtgt
     9721 caggcaaccc aaacccaaca gcagagcctg ctccgggtaa aaaccaggtc ggatttgttg
     9781 atctgggatc gggattgttg ctttacaata aaagtgctta ccttggattt tcattcaatc
     9841 atatcaccaa tccggtagtg tcgttttatg gcaccgacaa cagcgaacta cccggctctt
     9901 tgaatgcgca ggcaggggta tttattggtg gaaaacgtga aaaagacctg cttgtaaatc
     9961 cctacgcttt atggtcacat caggcaggtt tcaatcaaat acaaacaggt gtttatgtaa
    10021 aaaagagtct tttgttgacc ggtttggcag taaaacacaa tactgttggg cttagtgacg
    10081 ttgttttgct tgccggtgtt agtcaggaaa cgatacgttt tgcctacagt tacgacgtca
    10141 gcacggggcc tttggcaggg cttaccggag gggcgcatga ggtgtcgctc gtcattaccc
    10201 tgaaagaaaa cgccggaagg gctttcagaa acagtcaaaa aagtatgctg gactgccctt
    10261 cgatattgta aatctcaaaa taagtttgct acatttgcgt taactctcaa gtaaaaaaga
    10321 aagtatgaaa agaaacctgc atcctgtatt ggttctgtcc atgacagtcc tggtgttggc
    10381 ttttactacc tcatgtaaaa agaggtcggc agtttccggc ttcaaataca atgattccaa
    10441 atggggtggt ttcgaagcac atcgctttga cggtcaggct acaggcccca acctggtgct
    10501 cgttcagggc ggtactgttg ttcaaggtac cactgaacaa gatgttacct tcgaatacaa
    10561 taacatccct cgccgtgtga ctgtttcttc attctacatg gatgaaacag agatttcgaa
    10621 tctccactat cgtgagtatg tgtattgggt aaccaagcag ttccctgact tccctcaggt
    10681 aatcaaaaac tcattgcccg atactttggt atggcgtgag gaacttgctt tcaacgagcc
    10741 gtatgtgcaa tactatttcc gccacccatc ttaccaaaac taccctgttg taggggtatc
    10801 atggttacaa gctactgagt tctgtaaatg gcgcagcgac agggtgaatg aaatgatcct
    10861 tgccgaaaag aaatatattg atgtaaatac catgggtacc ggcgaagaca actttaccgg
    10921 cgaaacctat atgacaggtc agtatactac cggtcttcgc aaaggcaaaa aagattattc
    10981 tccttatgct gccgatccta aaatgggtcg caccgtgcgt atggaagatg gtatcctcct
    11041 gcctgactac cgcctcccga cagaagctga atgggaatat gcagcactcg gacttgttgg
    11101 taatcgcgtt ccgggaaatg agatgatcac tgatcgtcgc ctttatcctt gggatggcaa
    11161 cacccttcgc gaaccttccg cccgtgagaa atgggctcaa ggtaaaatgc ttgctaacta
    11221 caagcgcggt cgaggtgact atgccggtat tgcaggtaaa ctcaacgata actctattat
    11281 tacagctcca gtatattctt tcatgcctaa cgattacggt ttatataata tggccggtaa
    11341 cgttaacgaa tgggtagcag acgtttaccg tccgactact tatggcgaaa tggaagacct
    11401 caacccttat cgcggtaacg aatatcaggt gcgtgacctt gatgaagata acttccctgt
    11461 tatcgataca ctcggtcgca tccgctatcg tcctgtgaaa gatgaagaag ccgttaaccg
    11521 tcgtaactat aaaaaaggat atgtggttga cttccttgat ggtgactcaa catcagcagt
    11581 tacttacgat tatggtcgct ctacattgat cagcgataat gctcgtgttt ataaaggtgg
    11641 ctcttgggct gatgaagcct tctggtgctc accgggttca cgccgttatc tggaggaaga
    11701 cctttctaca gctactatcg gtttccgttg ttgtatgatt cgccttggtg gtgatgtaga
    11761 caacgaaact aaaggtggta acaacttcaa aaagactaaa aagcaacaaa agcttaataa
    11821 gaccaacagg aatatgaata aattctaagt tggtgcaaaa cttactgcaa agccttccgt
    11881 gattgcggag ggctttgtta tttttagggc atgattgccg agctctactc ccgcttctcc
    11941 catgcaaaag gcgttaccat cgataatcgc acgatctcgg gaggggagat gttcttcgga
    12001 ttgaggggtg aaaaagtaaa tggtggcatg tttgctgccg aagctttgaa aaatggagcg
    12061 agctgctgcg tggtggatga tcctcacttt gctactgatg ccaatattat acttgttccc
    12121 gatgcattgg tggccatgca ggaactcgca gcacattatc gaagtacctg gcaatttccg
    12181 gtgttggctt taacaggttc taatggtaaa acaactacca aagaattaat tgcagccgtg
    12241 ctctctaaaa aatatcgcac ctatgctacc aaaggcaatt taaataatca tatcggtgtg
    12301 ccactcacca ttttaaatat tggcgccgat tgcgaatttg ccgtgattga aatgggtgca
    12361 aaccatcagg gagaaattgc atcttactgc aaatatgcag atcctgattt cggactgatt
    12421 acgaatatcg gtttagcaca tcttgagggg tttggcggta ttgaaggtgt tgcgaaagga
    12481 aaatcggaat tatatcaata catcattaaa aaggatggtg ttattttttg gaacagcaat
    12541 caggagaggc taaccgatat tgcgaaaact tatgcgcgca cagtacgtta tggcacaaat
    12601 cctgaagatt ataccatagg aattattgat gaaacggcaa ccatggcaac agttgttata
    12661 gatgatgaaa ttaaaatttc cagtcattta gttggagcct ataatgccga taatattatt
    12721 gcagccgctt gcatcggaaa atattttgat gtgagttatg gtgaaataca agaagctgtc
    12781 gaaaactata ttcctgcaaa taatcgcagt gagcatcgcg agataaatgg taattatttc
    12841 atcctcgatg cctataatgc caatccttca agcatgcgtg tggcactgga gaatttcgct
    12901 aaaatatcgg cacaaaaaaa agttattatc atcggcgata tgctggaact gggaacatat
    12961 tcggcagatg aacataagca gattctggat attgcattgc acagtaaatt cgatcatatc
    13021 gttactgttg gaccgcaatt tcatcacatt cgccgcgatc cggttattgc atgtcataat
    13081 agtgatgagg cgcgtcagtg gtttctggcg cagcaattca gagaagcaac aattttgctc
    13141 aaaggctcca gaggcatgaa gctggagaaa ataattcagt aagtgtcgtg aatcgtgaat
    13201 cgtgagtcgt gaatggtgag tgggtgagtg ggtcagtggg tgagttaaag tatttggact
    13261 tagcgtcttt gcatcttagc tgtctttgcg agaaagaagg acaaagagtt gcacagtgta
    13321 aatataaagc acattctact gcatttgtct cactactcac aacgcactac cgactcgtga
    13381 ctcgtgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag cgtcaggttg
    13501 aggtaggcat taaactctgc gaaaatctgc gggacattaa acacacattc caaaatacag
    13561 tggctagcaa agacgaggtt atgaagattc gaaggattga agagtcgaag attcgaagga
    13621 agtgacctta attctatatt ccttattatt ttcattcacg agtcacgact cacgcttcac
    13681 gatgctcacc cactcaccca ctcaccccta agacaattta tgaaatatac gaagccactt
    13741 ctccggcact tcgttcttga gtgaattttc gtaatcttca tatgaacaag gaacaagctg
    13801 atgtcgctcg aatctttgtt gcacaataaa aggtacttca acccaccatc ttccgctttt
    13861 catgcttttc caaaacgtga gatcgtaatc gctttcagca acattgacga tatatttagt
    13921 gaagtctttt tccgaaacaa tagggtaatc gcttttccga ttataaaagc catcaacaaa
    13981 ataccaaatc atttgcgcaa ccagttttgc agtcaactcc tgattatcaa acatgggatt
    14041 atattcataa atacccagcg atgtgatttt atcgctcata ccggcgtacc gcatgatctg
    14101 acatatttcc catccggata atccatttgg cgtagcatga gcaattcccg gcgcatcggc
    14161 acctcgtatg gctccgatat caatactcag aatatcggca tcgcgcactt cgggttctgc
    14221 aaattgaata ttttctttta atcgccccag cctgatggta tcgaaaagca gatcgtcgat
    14281 tgcacctagc atttccgggc tgttaaaatg tgtctgatat gcgatggcag aataattgaa
    14341 caggaatttg ggatcgtaac gaaatatttg ttgcaaccag gttcttcccg gaggcagcaa
    14401 ctcagtatca tccagatcaa cccgttcatc aataaccatg aggttaactg cttgtcccag
    14461 aaactcatat cctgcatatt ggccgatagt gatatcctga cttccaccaa taagaatcgg
    14521 taatatttgt tgtccgagaa accattccat caccattttc acattttggt aggtttcgtc
    14581 gggcgtttcg ccgataatta catcgcctgc atcgatcatt tgcaggggat attcgctgat
    14641 agcgagttga tatagatggc gacgaacggt atctgctcct gtattgcatc cgctgttttg
    14701 catgctaccg cgactttcat ttacaccaat caatgcgata aggcaattat tcgtatccgg
    14761 aaattcgttg gtatacatgc ggatatgccg tgcccaatgg tagggctgta tggtccagtc
    14821 ttgaagaaaa tctgaaatac gaacaggagt gagcagcttc gtgatcatgc gttaaaattg
    14881 cctccttttt gctgaattaa ttaccgtgtt ggcaacagta aaggcgcatt ttgtctaatt
    14941 attcggtcat cctgtaaacc aatcaaaact caaaatatgt tccaagatgc gatctagtat
    15001 atatttttgc agtgataaag gcaactcgca tgcaagactt tctcaatcac gaatttttac
    15061 acaacacctg gcgacagtgg tgctggctga ttggcgtgat catctttgtg gtgatattta
    15121 aacgctatat ctcgaaactc atcagtatgt tgttgtttaa aatagttcaa cgtacagcag
    15181 ccggtgagtt gtcgcaagaa tttttaaaac gcattctcaa accgctgcaa tattttattg
    15241 tattgcagac tatttatatc ggattccata cccttaatta tccattcaat aaggaaaatg
    15301 aagagggtat ggcattagta cagacaatac tgtttggtgc atatcgcttt ttgatgatat
    15361 tgaatatcgc ctggatcgtt tcacgcatcg gcgatttttt tgtgatggtg ttgaaacatc
    15421 gcgcagaaaa aactgaagat ccatgggatg accagattgt tgttttcttg aaggatatca
    15481 ttcgattatt agtttggacc attgcgatac ttagtattct tggcgccgta tttggtgtaa
    15541 atatttatag catcattgca ggagcgggaa ttgcaggaat tgccattgct tttgcagcac
    15601 aagagagcct gcaaaatatt tttggcagca tagccatatt cacagagaaa ccatttgtaa
    15661 ttggcgatct gattgatttt gatggcacgc aaggcacagt ggtgaaagta ggctttcgtt
    15721 ctacccgatt gagaactatc gatacagcct atatgactgt gccgaataaa accatagtca
    15781 attcaaaact cagtaacctc actcgtcgta cttcacggcg cgtgtatttt acaatcggtt
    15841 tatcgcatga tacttctgta gaacagttgg aagctattcg caaagaaatt tatgctttcg
    15901 gtatgcagca tcctaagcgg aatgacgagg tgaatatcgg tgtaacacaa tacaataccg
    15961 ctgcaattga aatgcttgtt gaaattcatt ttgagtatgt aaactggtcg gcatttgtgg
    16021 aattgcgaaa cgaattgatg ttggcaatca tacagattgt taaaaaacac ggagccgtct
    16081 tcgcgcagca attggctatt gcagaaaaga aataaaaaaa accggatagc cttaactacc
    16141 cggttgaaat tctagtcagg aaattagcga cgctctttaa tgtctaacca tgcatttttg
    16201 catgccataa gcatcgctgc tcttaagttc aatgaagtat aagccactat tcaatccgcg
    16261 tgcatcgatt ggataactta ctcccggttg catattattt tgctgataca ccatgcgtcc
    16321 actgatatcg cgaacggtag ccgtaatatc gttggcataa gacggaacaa tatatacatg
    16381 tatataatcc gttgtcggat ttggataaac aagcatatct aatacagcgg catcagcgcc
    16441 aacagatgta taggtagctg ctgattcatc aataacctga tggcctgaaa atgtaccggt
    16501 attcccttcg gcaacgcgtc cataataact tccgcctatc acataaggga aagcgggttt
    16561 acctgattca tttatcgttg taaaataggc ataagtgcct tgagggtatt caggtgttat
    16621 gcagaatcgg ccattgtgtt catccagatc accggagcct gcaacgtaaa tataatcttc
    16681 aaggtaacgg cctaatgggt agttgttact gacatcgggt ccctgatctt cggcagaaag
    16741 catcgtacca tcagggagcg aatttctcaa agtgatattt cgcaattgat agctcgaacg
    16801 catgcgaaca atattgccac tgccatcggt attttcgaag gcataggcac catatatcgg
    16861 ataaccatca aaggcgtaac ctagtattgt tgcgtgctct gtattttgtg ttggatcgaa
    16921 aagacaggca ggagcggtat aattataata ttccccattt tcggcaatgc gacctgtgca
    16981 tgatggttct acctctgcat tctcggctac ggcgtctaca tgccaaatat tgttgttgtg
    17041 ataactctgt ccgcttcgta tatcaaaaat gctcagacca ttgctccaaa caccgatatg
    17101 acccggttgt agtggggtag ggtctgcagc ttgcggtaag cgcggaatac gaaaaacaaa
    17161 attttgattt gaaccaagtg cggggtcatc ccaaggtcct acatcatagg ctgcaatggc
    17221 atcgcaactg ataaatacat cagcatcggt gtattgcact tgctgcacat tggaataaat
    17281 gatgtcatga aaattggagg atcctgcgtc ggtgatccag ctggtgattt ccggtgtcag
    17341 ttgggcgcga accgtgacga cggtacacac catggacatg atgagtgtag tgcttttctt
    17401 catacgttga actttagata acttgaaccg gacttaaaat taccacactc cttcgatgga
    17461 gggaattcac gtaataccat gtgtttcata agtttgttca ttaaatgata caattgttag
    17521 gattcgacat cagaaatacg atgtataatc aagctcggtt gcaaggtgtt tatcaacaca
    17581 gtgtgaataa tccccgattt tatggcactt tatgtgaaac ttgatatggt agtcacagtc
    17641 gcggctagta tctttgcact ttaatggaaa acttcgagaa agttccggcc gtgctgctct
    17701 tagctgacgg aactgccttt tacgggttca gcgccggcaa aatcggcacc actaccggcg
    17761 aaatctgctt taacacagga atgacgggct atcaggagat ttttaccgat ccgagttact
    17821 ttggacagat cctggtgatg accaatgcac atattggtaa ttacggcacc tattctgagg
    17881 atgttgagag cgaaaacatt aagatatccg gtttggcggt gaaaaacttt accaaccaat
    17941 ttagccgacg gatgacaacc accacgatac aggattacct gctcgatgaa catcttgtgg
    18001 ctattagcga tgtagatacc agagccatcg tgcgtcatat ccgcagtaaa ggcgccatga
    18061 actgcatcat ctccagcgaa acaactgata ttgaggtgtt aaagacaaag ctggcagaaa
    18121 cgcctgatat gaatggtctg gaactggcaa gcatcgtttc aacaaagaca ccatatttta
    18181 tcggcaacag cacagcagaa aaaaaggtgt tggtatacga tttcggtgtg aagaaaagca
    18241 tacttcaaaa tattgccgac cgcggttgct atctcaaaat agtgcctgca aatacaccat
    18301 tcgaagaaac tacaagtttt gatgccaacg gatattttat ttctaatggt ccgggagatc
    18361 ctgcaagtat gggttatgcc gttgatacgg tgaagaaaat tcttgcagca gataaacctt
    18421 tattcggtat ttgtctcgga caacaattat tggcacttgc caatggcgtg tctacatata
    18481 aaatgcacca tggacataga ggatgcaatc atccggtgaa aaatcttgaa acaggattat
    18541 gtgaaatcac cactcaaaat catggattca gtgtgcgtga agatgagttg cgtgccaata
    18601 ataatctgga gatcacacat atgaatctaa atgatcaaac ggtggaggga tttcgtttaa
    18661 aaaataaacc cgccttcagt gtgcaatacc atcctgaggc aacacccggt ccgcatgatg
    18721 cacgttatct gttcgatcaa tttgtgaaat ccatgcatgt ttaaatcagc aattaaagaa
    18781 ctttatctat catgagcaat attattcata tccacgcacg tcagattctt gactctcgtg
    18841 gaaaccctac agttgaagca gaagtattaa cagaatttgg tgcaattggt cgcgctgccg
    18901 ttccatcggg agcatctacc ggaatacatg aggcagtgga gctgcgcgat gaagatcctg
    18961 atgtttatct ggggaaaggt gtgttgaagg cagtagaaca tatcaatgaa tctattgtgc
    19021 atgaactcga aggcgtagat attttcaatc aaaatctgat cgatcaaatt atgattgaat
    19081 tagatggcac accgaataaa tcaaagcttg gtgccaatgc catgcttgca gtatcattgg
    19141 cgtgtgccaa agctgcggcc tctgaattga gtttaccctt atatcgatat gtcggcggtg
    19201 tgaatgccaa tacacttcct gttccattga tgaatattct caatggtggt gcacatgccg
    19261 ataataaaat tgatttccag gaatttatga tcgcacctat tggtggtgat aatttttctg
    19321 acgcattacg catgggtgtt gaagtttttc atcacttgaa aaaaattctt aaaaacaaag
    19381 gttattctac caatgttggc gatgaaggtg gatacgctcc gaatattcaa acaaacgaag
    19441 aagccattga aacagtatta atggcgatag aaagtgccgg atatgttccc ggtgaagata
    19501 tcaccattgc catggatgct gctaccagcg aattttataa tgcggagaaa ggactttaca
    19561 tctttaaaaa atctgacggt cgccaaatga cgcctgccga aatggtagct tattgggcag
    19621 aatgggcgaa gaaatatcct attacttcta ttgaagatgg ctgcgctgaa gatgactggg
    19681 aaggctggaa attgatgacg caacagcttg gaaataaaat tcagcttgtt ggcgatgatc
    19741 tgtttgttac gaatgtaaaa cgcctttcaa aagggataaa agaacatgtc ggtaattcaa
    19801 ttttagtgaa agtaaaccag atcggcacgt taacggaaac gatacaggct gtagacatgg
    19861 cacatcgcgc aagatatacg tcagtgatga gtcaccgcag cggggaaaca gaagatacta
    19921 cgatagctga tcttgcggtg gcactcaatt gcggtcagat taaaaccggt tctgcttcgc
    19981 gaagcgacag gattgcaaaa tataaccagc tgctgcgcat agaggaagac ctcggaagca
    20041 tggctgtttg gccgggaaat gtattcctgc gttaacattt gccgggactt aaggccgaat
    20101 ttaacttacc gtgtgggttt ttgtgaacaa tccacacggt ttttttattt accggcgttt
    20161 atggtatgta acctccacgt atctttatca ggtcatgaca acaaaaaggt caggttttcg
    20221 ccaacgactc aggcaatgga aagtgcctgc gctgctcgta aataaatacg tgtttaccct
    20281 gtttgtattt ggcgtgtgga tgacattttt tgatcagaat aatttcatga ggcaatatag
    20341 gcgtattgct gaattaaatg cagtgaaaag taaaacagcc tattacgaaa gcgaaacagc
    20401 aaaggcaaaa aagcaattgc aggcactcca gaatgatcag aaagctctgg agcgtttcgc
    20461 acgtgaaaaa tactacatga agaaaccgaa tgaagatgta tttgtcatca tagacaaata
    20521 attcattaat tattaccggc tatttgttat ttgtcaatat cctccgctgt attttcggat
    20581 gaaccactct acgctgagta ataacaatat cacggcaaat atccatttca aatgaataat
    20641 tgattgtgtg cgagtgattt cgcgcatcac cggttttgct tgcgcactgt tttttatcgc
    20701 atcaaccagc gatgccatat tttcaggata ggccatactg cctccggttt gaacagcaag
    20761 ttgattcatt aaccgatggt cggcagtaat attgatagtt tccaatctta caggcgacac
    20821 actaaaagct ccggcatcag tatatgtttt gccattgaaa gttgtggatg ctttgtaact
    20881 gtaattgcct ggtgaaaaat atcctgcact taaagtataa ctatttccgg ttttggaaaa
    20941 ttgaaaatca aaatcttttc cggattcatc tgtaatggta atggtggcat caggcgtatt
    21001 aatcaattca taagtgtcat tatataattc tgcatcaaga ataatatttt cctgctcagt
    21061 ataaacagcg cgagcttgac ttacacggaa ttgtttcttg tcgtttttag cagcaaggta
    21121 ttgcactgtt ttaccaatga tctcatcaga agcttcttgt gtgttattaa gcacgtagtc
    21181 atacaagcgc catttccaaa tattttctcc acataatacg gcggtttttt ctccacccgg
    21241 aatgctaaac aataacaatg gcgttttagt agccacggat ccaattttct gataggccac
    21301 cacctgcgat gcctttgatg ccgtgtattg accgtaaggt gcacttagtg ccggcaatct
    21361 tgggaggatg ccaagcgttt tatcatcaat ggtgaagaga ttgaattgca aatccattaa
    21421 cgccgtaacc tcatttgcag attgcccatt gctattgata ttgagcagcg actgtgcctt
    21481 gttgaaagca acaactgatg tttggctgcc tgtaataaac caagtaggaa tacgcttcgc
    21541 ctgcacctca tttatcacct gtggaatagt atttgtggaa gaaggcaatt gatgtaaaat
    21601 gacaaggtcg tagtcggcaa tattgccttt ccattcgcct gcaagctgca catcgacatc
    21661 ataatttttg ttttgcgtga tcgataaacg caaggcttgc agatcgggat gtggtgcgtt
    21721 ggcgagtaac agaatttttt gtcgggcatc gagtacttcg atataaatat cctgcacgtt
    21781 gttttctgtg gtgaattcac cttccacctt tctcacattt attcgataat gacgaattcc
    21841 gggctcctgt gcagcaattt cccattttac gcttccattg aattggtcac caacagcatt
    21901 aacagattgt tcacctaagt ttcgattacc gtttttatta atttctgaaa ttgaagcact
    21961 gatatttata ccattgcaga aaatcccttc aatgtcagcc tgcacagtaa aacgatcatc
    22021 gagatacacc aggttattgt gtaaagtgcg aagaatgcgc acatcttttt ttcgtgtagt
    22081 atctcccagc gcaacggtat aaattggggc gttgagttca tttttgataa ataccggatt
    22141 gcttccacta ttataaatac cgtcagaagc caatatcact cctgccacat gttgttgctc
    22201 atacaaatta ttgagtgata acagcgattg ggaaatatta gtggatgcct gatcgaaatg
    22261 attttcgcca tcttcctcca ttgtttcacc gaatacatat tttctaacgt caaaatcttt
    22321 tgataattca gttgccatag attcccatgc atccaaaaaa gcagtagtat cgccaatgcc
    22381 ttttgcaata ctggaagaat tatcctgcaa caaaacgaca atgggtttct ctgtttcatt
    22441 agtagatgaa cgcatataag gattgagcag caacatcaac aaaattgtag cgccaataaa
    22501 ccgtaatgct gcgggtatcc accattgttt tggcagcact tcagctatac gtcgttcacg
    22561 ccagtataat gcagcggcat atagtgcacc accaagtaca cataaaaacc aaaaccacca
    22621 ggggtaatca gagaaaatct gtaactgcat aaattgccgg agtatccgct tctatgtgcg
    22681 aatataaggt aaatacaacg agtaattgcc cgtgttagtt gtagtaaatg ttgttaagat
    22741 tcgttaccgt aatctttttc agatcaggta tgtaaggcac cgcaaacact gattacctgt
    22801 ccggtaacat aactactcat ttcactggca aggaaaatgg caacatcagc cacttctttt
    22861 ccttgtccga agcgtcccag tggaatagat ttaaaataag tttgtttgag ttcctcgctc
    22921 aattcgccgg tcatatctgt ttcaataaat cccggcgcaa tggcattaca acgaatattt
    22981 ctggaaccga gttcttttgc aatggatttc gtaaaaccta taatacctgc tttagatgca
    23041 gaatagtttg cttgacctgc ttgtccgatc aaacctacaa tactggaaat attgatgatg
    23101 ctgcctttgc gtgccttcat cattggtttg atggcaaatt tggtgagatt gaatactgat
    23161 ttcaaattca catccataac tgcatcccaa tcttcctccg acatacgcaa cattaacgtg
    23221 tctttagtaa tgcctgcatt atttaccaag gtgtcgatat tgccaaaatc agctatcaca
    23281 tcattgatca atttttcggc ttctgcataa actccagcgt tacttttata ccctttagcc
    23341 ttcaccccca tttcagaaag tttctgctcc aatgcctgcg ctcttgcttc gctgcttaaa
    23401 taggtaaatg caacattggc accggcctca gcaaaacgca tcgccattgc ttcgccgatt
    23461 ccacgtgagg caccggtgat taaaacagtg gtatttgtta gaagtcccat aggtttggtt
    23521 ttgaagttcg aagggggagg taattggtat caggtatcag gtatcaggta tcaggtatca
    23581 agtatcaggc ttgcctcgcc tctggcgggt atcaggtatc aggtatcaag tatcaggtat
    23641 caagtaatta gtgtcaggct tgtctcgcta tttgcgggta tttgttgttt gatagcttga
    23701 gccaagtatc agttactaat tgggtgtttt gaaacctgtt gcgatttttt gggtttgctt
    23761 ttgatgttaa attctgcctt tcattgccat ggccatagtt tcgccaagta ctgcagggct
    23821 ttctactacg tggataccgc aattgcgcat gatttccatt tttgcggcag cagtatcatc
    23881 ttttccgccg acaattgctc cggcatggcc catggtgcga ccttttggag cagtttgacc
    23941 cgcaatgaaa cctacaacag gcttggtgcc atgttctttg atccagtttg cagcgtttgc
    24001 ttccatagtt ccaccgatct caccaatcaa tatgattcct tcagtttcag ggtcagccat
    24061 taataatttg atagcatcaa gtgtagtagt tcccggaaca gggtcgccac caataccaat
    24121 acaagtgctt tgtcccattc cggctttagt aacctgatct acagcctcat atgtcaatgt
    24181 tcctgagcgg gaaacaatgc cgatagagcc ttttttatgg atgaaaccgg gcatgatacc
    24241 cacttttgct tcctcaggag tgatcacccc ggggcagtta gggccgatca aggtgcatga
    24301 tttattgctg atatacgctt ttacacgaac catatcctga acaggaatac cttcggtaat
    24361 ggcaacaatc acttgaatac ctgcatctgc agactccata atggcatcgg cagcaaaggc
    24421 aggtggaacg aaaataatgg aagtgttggc tccctctttt tttacggaat cctctacagt
    24481 attgaatacc ggacggtcca aatgcttggt tccgccctta tcaggtgtta cgccgccaat
    24541 tacattggtg ccataagcaa tcatctgttc tgcatgaaag gtgccttcct taccggtaaa
    24601 gccctgaacc actaaacggg tgttttttcc aactaaaacg ctcatgccgc gaagataaga
    24661 cgatttgaga attgtttcac cgatagtg
//
LOCUS       DAOQOU010000032        24653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_957_length_24653_cov_5.451216, whole genome shotgun sequence.
ACCESSION   DAOQOU010000032 DAOQOU010000000
VERSION     DAOQOU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_957_length_24653_cov_5.451216"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="PLM90_04610"
     CDS             <1..1757
                     /locus_tag="PLM90_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88681.1"
                     /translation="ISWGTPIAILAGDATAYDDTDVIIDSMYEYKVKRTNISGLEGEG
                     YLLGAITLHAVDYRGTCLLVMDTTVTDALTNEITRLMDDISGDGWSVSQLHVARDMSP
                     IAVREAIIAAAEADPEMQSVILIGRVPVLYSGNLYPDGHPDHQGAWPADGIYGDINAD
                     YTDVTINTTTAGRPENWNTPGDGKYDQSIFKSKIELAVGRIDMYNMPSFALDETGLLK
                     RYLDNDHKFRTGITHFEQRALIDDNFGAFAGEAFASSGWRSYAPLVGNDNLQELDYFS
                     TMYDNSYICSYGCGGGWFQGAGGVGSTTDFTLDTVKTVFTFLFGSYFGDWDVNDSFLR
                     APLAGGYALTNAWAGRPHWSLHYMGLGYPIGYASRQTQNNQSTYTSNIFPKWVHIALM
                     GDPTLRMQVIAPVSEMHCEISDIQNAIDIHFTASADPEVIGYYVYRAATTYGKYERIS
                     DDIVATSPYTDLTPIDGLNYYQVRPVMLTTTPSGTFYNTGIGLRDSILIDVTDVNTLA
                     PSIVQLYPSPADTYIVVVTSSDLVGSTFIIYDISGKAVLTVKLLSEDTSIKTTTLPDG
                     VYTLKFSNGITRQFVVSH"
     gene            complement(1804..2682)
                     /locus_tag="PLM90_04615"
     CDS             complement(1804..2682)
                     /locus_tag="PLM90_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ88682.1"
                     /translation="MMKLRTVRALQYADMMDMGGMPVRQPLPSAAIDQVDPFLLLHHH
                     SSKIAAGAYPREVGVGPHPHRGFSPVTFIFKGDVHHRDSRGNSAIVHAGGVQWMDAGM
                     GIMHSERPSKELAEKGGSQEIVQLWVNTPAARKMDQPNYQALQENDIPSISIDDVGSN
                     IHVVAGTFEGVKGPAHSKLDLLMLNVTMKAGIKRQFEIPTDHQTLLYVVKGGLTMTGY
                     GPVPALHMVVLHQDGAGIELTADQETIFLILSGKPIDEPVIQYGPFVMNSQTEILEAM
                     RDYQMGKMGILIEEFD"
     gene            2804..3676
                     /locus_tag="PLM90_04620"
     CDS             2804..3676
                     /locus_tag="PLM90_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ88683.1"
                     /translation="MQAIGFYLLLPFLYFFSVLPMFILRQLSNVAFFIMYYITGYRRE
                     VVMRNLGHAFPEKSEQDLKKLARGFYLYLSDTLVETIKSFTISDAGLRKYCAINNYDV
                     VQAEIDKGRNVMFVLGHYGNWEWGGGALAKRAPIPLKAAYKPLSNPYFEKLMFHTRTR
                     LNIRLIAKRKILAHLKDHADEQTLLVMIADQSPNPKEHFWVKFLNQDTAVVTGMEKIA
                     RQYNYTVIYSTIASVGRNKYEIRFKKITDDPQSLAPEALTAMFMHELEQDIYKNPAPY
                     LWSHRRWKLKKPQA"
     gene            complement(3673..4293)
                     /locus_tag="PLM90_04625"
     CDS             complement(3673..4293)
                     /locus_tag="PLM90_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ88684.1"
                     /translation="MKRIMLILFCVAAVDLQAQEYELGVKGGVNYAQSVILNVVSLDG
                     VDVADVESQKGSALVFGGFARATFGRWIVQPEILFSENQSLVTLADANVNEMDLSDLF
                     SMSVDKCDVPILLGYQAFGSVKLMAGPVFTTVRSTMSDPLFSLKDMTVGYQTGINFDI
                     NKLTFDARYEGNLSKFKEYVETEKGMLEVDTRKSLFQFTVGYTLFD"
     gene            4398..5141
                     /locus_tag="PLM90_04630"
     CDS             4398..5141
                     /locus_tag="PLM90_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ88685.1"
                     /translation="MSATPEWNIRLEPRNIKYLSGPRSRWRELWWAFSVFTEFIKGFR
                     ALHFIGPCITVFGSARFKPDHQYYKQAYDIGKKMSALGLTVLTGGGPGIMEAANKGAF
                     ENQGRSVGCNIVLPQEQKPNPYMHKWVTIKYFFVRKVLLVKYSYGFVVMPGGVGTMDE
                     FFETLTLIQTATIHNFPVVMIGKDYYKDIWEMLQKMTQAGTVSPEDLNLVLFTDNAQE
                     AVNHIDFYIKKHYSFKKVKPRKLLLERFV"
     gene            5160..5294
                     /locus_tag="PLM90_04635"
     CDS             5160..5294
                     /locus_tag="PLM90_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88686.1"
                     /translation="MRIPEKTMMWLKRFGWAGFFFFLIKGIVWLIAGAAIIEWFQKTF
                     "
     gene            5396..7777
                     /locus_tag="PLM90_04640"
     CDS             5396..7777
                     /locus_tag="PLM90_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88687.1"
                     /translation="MKQITLFLFCLTIGTFARAQQLTQTIRGQVTDNQSKATLPGVNI
                     MVLNSDPILGTTSDLDGYFAIEQVPIGRVSIQATFIGYEPFIVNNLELTSGKELMLNF
                     SMSEQVITMNEVVVRAKDDKMETNNEMTTVSARQFTIEESMRYAGARNDVSRMAQNFA
                     GVRGSNDAVNDIVIRGNSPVGVLWRLEGIDIPNPNHFGDLGSTGGPVSMLNNNILANS
                     DFLTGAFPAEYGNGISGVFDLKMRNGNYKKHEFLGQVGLNGFEFGAEGPLSKDSKSSY
                     LMNYRYSTLGLMSAIGINFGTGTAIPYYQDITFRVNMPTRKAGTFALFGLGGVSSIDL
                     ISSSTEDSVDNLYNNDLDIYDKSKIGMVGFTHQYLINDKSYTRFTLSTSSIENTDIID
                     SISSENFEPINYYNQNFTQHKASASLMYKIKLNSRQNLQAGASADMLFADLNDSIYRN
                     TLDRWITITSYNGNTMLLQGYVQYQIRLTENLTATAGVHDQFLALNGSNSVEPRAGMK
                     YTINEKNAISFGYGLHSMMAPISVYFKTVETTPGNYITPNDSIDFIKAHHFVLGYDRQ
                     LSSTMRFRSEIYYQHLYDVLIDRDSSSYSVLNTGSMTFGSPDYLVNKGTGRNYGAEVT
                     LEKFLDKGLYFLVTGSIYKSEYLGSDDIERETAFSGNYVANVVGGKEFAILAGRAGAK
                     TKKSVVFDTKLTMAGGQRYTPIDLTASIDEGKTVYDWDKAFEAQFDDYFRWDVRLAFK
                     MDGKHASQEWAIDVQNVTNEQNPLYQRYNPVSQTVNTIYQLGIFPVPQYRITF"
     gene            7774..8835
                     /locus_tag="PLM90_04645"
     CDS             7774..8835
                     /locus_tag="PLM90_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ88688.1"
                     /translation="MSKQSYIPFRKALAGTLVVSLVPLIYQILVYYIVGTPKCPELTF
                     WENAMWTAVFSLLVTFALFIVNIYINTKGHELLEEKVNDGIRIFVTVFVSLLASNIVI
                     FVMWKLFNDLILHLPEDGETARVFSNQILATVLVIIVDLIIEVDIYVKKLKISMETQA
                     RLEKEFIRAQLESLKTQISPHFLFNSFNALQSLIDQSPVQAKSFIQELAKVYRYVLDK
                     KDEMVVPVSDEVAFIRSFLYLNHIRFGDSLSADIDLSAQEMDGFIPPLTLQLLVENAI
                     KHNIISQARPLHISVFRDAGNIIIKNNLQLREDTPSASGIGQSNLVARYNLISDRTPS
                     FNIVDDAYIAKVPVLEQEL"
     gene            8840..9586
                     /locus_tag="PLM90_04650"
     CDS             8840..9586
                     /locus_tag="PLM90_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ88689.1"
                     /translation="MHVLIIEDESLAANWLRTQIEQSTKDMTVVAVLDSVSASVDWLQ
                     KNQVDLIFSDIHLADGISFSIFERIAISTPIIFTTAYDHYAIRAFQVNSIDYLLKPID
                     TEALSASIEKYRSLHTNIQPDWKMLVSGLQPKNNYQERFLVSTGQKLKSLPIIDIAYF
                     FADNKLNFVCDTNGRTYILDQTLDKLEQVVDPGTFFRINRQFIVSFGAIQDMLHYSKS
                     RVKLTLHPSTEHDTIVSAERSAAFKAWLNR"
     gene            9635..11908
                     /locus_tag="PLM90_04655"
     CDS             9635..11908
                     /locus_tag="PLM90_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88690.1"
                     /translation="MKYWMLLSAIALASCSATLENEPITHTNWPTREELFQNFRNQDT
                     AIIVYGKEDYKISDLLVNGVAALAPKDDRFTVITRRDDKLTEFEAANYPLYIIGTRNN
                     LLIKRVSEAIPVKVFEGGFNFNGTDYMAPNDVIKLSFYPNVFNPKIPFTVITGNHEEA
                     IVQYVQQEMNGEWGYFFWDNWGYQVYHNGRRTILGNFSQDSASLWSIDKKQHWEFDFE
                     GKSLKTSGQVHYTGHNDPNVTNLDNWDAVAQDDLTRISEFCGKKISARLEVHLYPTPE
                     TKTLLMSDPDQSTIDYAAYAIHTVISGEYENHRDEKLNMLALRNMMGNAGCVAYEEGL
                     SVLYTTNWQNEDIRLLALKLRQANALPSLTLLLHNDAFEGESDYLMHIAAASYVDFII
                     NSQGKESFLAQYSQKPVITAELETAWYQYIDDFGKRIRIAPKPKPALPEHLKGFNFAH
                     EGYQIYNGYMGSEAEHSIAKLKEIGANTIAIIPYSGFQSMDKPYRFRYTNGPGAENDA
                     AVVHAGYVAHQQGMVTMLKPQLWSWLGWTGDIKMQNAADWDLFFEYYEEWIMHYALLA
                     ELYDYDILCIGNEFQAATLSEHARWDALFEKVRLVYHGNITYAANWGSEFEQAGFWDM
                     LDFISVNCYYPLSEKTSPTDAELLAAFEHNLDIIEKVQKKYNKPVIFTEIGFKSIASP
                     WIAPHKDADEQAYDETSQDRCYQVMFKAMADEPWIKGTYIWKWPSYMDFSKEYSKDFN
                     PCNKLAEKTISENYKKQ"
     gene            complement(11916..12542)
                     /locus_tag="PLM90_04660"
     CDS             complement(11916..12542)
                     /locus_tag="PLM90_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88691.1"
                     /translation="MIKRIKYAVLALVLTMSLTVKADSPITSTYIATFYYEYPIVGIA
                     ETTRGLNDEIAAYLLDPDNLIDVKAAVINAIGWNYDGTQNADLFKTYLAKAHNTTTQQ
                     LSMSSLSGDELFCLGYFMAMDNYFVVDDAINVLQMAADKNSTSFTTHLFLGMVQAQKA
                     MDYDFCQVWRITASVINDKSLTRDLKTGAIQSIVDYMILYKGACVADY"
     gene            complement(12535..13164)
                     /locus_tag="PLM90_04665"
     CDS             complement(12535..13164)
                     /locus_tag="PLM90_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88692.1"
                     /translation="MPDNTYVLVAARMTDEDLLEALAHHEKYVDDMLTAMLDVAEKRG
                     LTVLNAEALRAEIAGRIEPEEILQAIDEERPIPIELPELYSQTAILAFSIFFSPAFGG
                     ILFAMNMKRMQKKNGWLVVLLSFAVSAISGYVTWYYAKGTFLGILIPIACGLLLSEIL
                     WSRIFGKHQPYKHRRVIIPLVIALAIAIPLSYYMYEHPEQFGPQTTQHD"
     gene            13191..14060
                     /locus_tag="PLM90_04670"
     CDS             13191..14060
                     /locus_tag="PLM90_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88693.1"
                     /translation="MNKSVKLLLIICFFTALPAFAQTQLNTKGLFTFVRVLPIPSDFE
                     IGPGQINKDGSSFFVGLTDGDLENMEDLHSDVYRVNVTSDVNAIPITGLEIPNAIDSL
                     RFFQASASDNDAYIVFVVNAWAGWNDNELAIVKRQPSGAFGGMRMLTEVNKEHFSDAY
                     PWLSGDANRLYFSQDFKLMYSERSSPDSLFSSPKPVDFVGDVQLEIVSAWLPPDEKSI
                     FLLANNRIYKSTRKSTTEPFSFPSLWTDEFKDFYFVAGLSFAPDKKTMYIYHSDETSQ
                     HILQYQLSKGKAW"
     gene            14186..14596
                     /locus_tag="PLM90_04675"
     CDS             14186..14596
                     /locus_tag="PLM90_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88694.1"
                     /translation="MPPTLYPDKSISEIAQMAYTDSLHHSFLMVLRDVTPDLEEDSIF
                     ITHQRYHVYACAGIESAMLNADKDDTDSVMTGGNLVYTTPIEGEFGGHEVHYRVSTIE
                     TEYYFYQILTWHLADNDAMEKQLYDAMLSFRPFR"
     gene            14617..15360
                     /locus_tag="PLM90_04680"
     CDS             14617..15360
                     /locus_tag="PLM90_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88695.1"
                     /translation="MINFKNTGFAVIAILMTASFISCGDENQADEKTPAPLNRSMFYG
                     KITYTSTTSSPDSVFLATMSTFSPHAVDIYFGKSAFRMVEHGGLSNGNILVLPESGEA
                     WQLDSTKMIAYLGEYSDLGDPSDALKDLMPDHFAPTVKATGKTANILGIPCTEYYIVR
                     SGIIPKEDSATIMVANTINFPSSRYDVQTEINHAAVPAPLFLGYEDGAIMQMTVVNKR
                     YTRKFEITALQENYFPEGIFDIPANFQKK"
     gene            15357..16382
                     /locus_tag="PLM90_04685"
     CDS             15357..16382
                     /locus_tag="PLM90_04685"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ88696.1"
                     /translation="MNRLKLLTGSFFLAQTLMAQSVQVDSVTAPSSFRGMAVVSDAEI
                     WISGSKGYIGHSIDSGKTYTFHQIPKYENAEIRDIEFLNDGALVAMSSTQPAAILRST
                     DKGATWKEVFRRDTSAYFLDAIDFSGKQGTCLGDPINGHFLILHTSNYGKTWTETYTN
                     IATDSLAAFAASGSTLQYLSTNEIVFGTGGKEALLFYSKDAGKTWETTEMLMNSGKES
                     AGIFSVAVIDSKNIVCAGGDYLDPTNTDYNYLLVYIIEGVWDYPIPVSEINKESSGYY
                     SCIDVITNNNITTFFACGTGGVDVWRSTNFKIKVLSMSGFNVLQISPSGKYVFLAGNK
                     IVGKMDI"
     gene            complement(16393..16935)
                     /locus_tag="PLM90_04690"
     CDS             complement(16393..16935)
                     /locus_tag="PLM90_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88697.1"
                     /translation="MFQMIRLKNVVALSAICCIGFSGLYAEELVGNQPSSIDKSADYS
                     AGSNTVVYVETDMSTPGAVINADNTFIDAVDAVVKEDTGKKEEQEPVLEPMVLAGTGA
                     AVAGAGVAAAGMFAAPILLAIGALLLIASAILTSKGWKKIKAEPKKYKGEKFAIVNFI
                     IIGVLGVAASFYALYLLFMV"
     gene            complement(16957..19176)
                     /locus_tag="PLM90_04695"
     CDS             complement(16957..19176)
                     /locus_tag="PLM90_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMZ88698.1"
                     /translation="MALINLDGANYYVSPAGNNLNSGIGIASPWKTIQFALNQANDGD
                     VINVMAGTYTGKLTWNDGGVVGQYITLQNYNDDVVILDGSTVGNNQALMYIENKNYIK
                     IDGLRFTNHNGNYQPVINVYGNCSHIEITNCEFYNTNCNESYAILCEGFGDDFLISGN
                     NMHDLIGDNAVGVLFVGSGTTTAYTNIVITNNVLTNLDPAPSEAIAVNGNIDGFTIAD
                     NVLTDINNIGIVMIGGEDWVNTNDAVNQARNGVCRRNAVTAANSIYGGGFSAGVYVDG
                     GKNIVVENNSITDSDIGLEIGCENMGFVCSGITVRNNLIYRNQKSGLGFGGYDYPATG
                     MVTNCAFYGNTVYDNDFLNTGFGQLWIQYATNCIVENNIFFTSTTPWMINAETIDSTY
                     EVNLDYNNYYYSGGPMAMKGFVDFTLITGYEALLSTMGDNAHFSLADPLFVNPAISDF
                     HLQVTSPCINAGNPSYIPVDEFDMDLATRLTGGRIEMGADEQIAAPLSYLVNSIPTIC
                     HGSCDGSLELDGIYGCAPYTYQYKIGAGAWIPFDGFLSDLCAATYKVKVTDACGAFVM
                     GNVVISEDALLTLTVTSIVNETVAGAHNGKITVQSTGGYGSKQYSINGGLTWQTAKKF
                     SGLSAGTYTIMTKDAHNCTASVVATVLVGGRMMDEAESMVYPNPANHEIHISTNQPEM
                     PTIIRLMDLGGKEVFFEETNGGEVLITLPESLPSGLYLLQWSNVLGLFQQQVAVASR"
     gene            complement(19294..20028)
                     /locus_tag="PLM90_04700"
     CDS             complement(19294..20028)
                     /locus_tag="PLM90_04700"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HMZ88699.1"
                     /translation="MKTIVHKLLIAILVPFSIASANPPAGWVPVANSTLEIKSQGSYY
                     FSVQLDGRYYPNPVKRFSMATMTAGMHHVDLFAAGQQGHAGAQKLIYSGDIYIDANSL
                     VTGVLDNAGRFYIKSVKSIPVYVPEPQTYYPPNTYPTCGTPPLPPKPLPMAGYSFTQL
                     MDVIEDQWFESGRLQIAKQALNSNWFTANQVAEMMHAMSFEDTRLELAKAAYYKTVDT
                     ENYFIVNKEFWFSSSVDELSRYLSGI"
     gene            20151..22076
                     /gene="dnaG"
                     /locus_tag="PLM90_04705"
     CDS             20151..22076
                     /gene="dnaG"
                     /locus_tag="PLM90_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMZ88700.1"
                     /translation="MIKKETIDRINDTARIDEVVGDFVRLKKRGVNLIGLCPFHNEKT
                     PSFNVSVPRGIYKCFGCGKGGNAVSFVMEHEKFTYREALLYLANKYNIEVEETERTNE
                     EILEDNRRESLYIINAFAQNYFSEQLLNSEEGNNIGLSYFEERGLRRETIETFKLGYC
                     PSDGDSFTQSAHQAGYQNELLQHLGLTTAYGKDFFRGRVMFTIQSVSGKILGFGGRTL
                     SADKKQPKYINSPETDIYFKSKIVYGIYQARKAIAQHDVCFLVEGYMDVISLHQGGIE
                     NVVASSGTALTQDQVRLIKRYTQNLIILYDGDAAGVKAALRGLDIALEEGMNVKVVLL
                     PAEDDPDTLVQKLGGDGFNKYVEAQKKDLIHLKTDLFLEEAKGDPVKVAGIIKDIVAS
                     IAKIPDAITRSLYVKQTAKMLEVEEQILITETNKLLRKEATKKVTPEDKATVEALDYT
                     PPAKEQPLFTFHLDTPQERDIVRLLLENSNYEIEGENAIARILRNLSDVEIDHPEYNM
                     IIEEYRNAFAQNEFLTQAHFFNHPDEAIRNLTIDLLQSPYELSDNWTKMHDVPITDKQ
                     FLVRKDIVKSISLLKLKKIIRMKQDVDNRIKELQHDTSENGIQETMLCQKESIQLQQF
                     IRKLSTDTGTVVLPGIL"
     gene            complement(22032..22856)
                     /locus_tag="PLM90_04710"
     CDS             complement(22032..22856)
                     /locus_tag="PLM90_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HMZ88701.1"
                     /translation="MDKHHHNPFASKPAILLLVLGVFFVVNAIIAEFIGVKVFALEDS
                     LGLKPFNWNLYGQAGSLNFTVGVILWPFVFIMTDIINEYFGVKGVRRLSYLAVICIAY
                     AFAIVYAGISLKPAAFWPGSFVDSGVPDMQAAYAAIFGQGMWIIGASIAAFLFSQIVD
                     VFIFHRIKAFTGERRIWLRATGSTAISQIFDSLIVLYIAFVLGPQHWTLPFFFAVATV
                     NYFYKILAAIVLTPVLYWVHHIIDNFLGTELSEKMRKEAMGISEDAGQNDSAGISG"
     gene            complement(22860..23309)
                     /locus_tag="PLM90_04715"
     CDS             complement(22860..23309)
                     /locus_tag="PLM90_04715"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMZ88702.1"
                     /translation="MVKDRILVFIQLVFFAVYIWEPWQYSFQPGIAGRLVGLLIGVIG
                     LILFWWPILQLRRNLTMYPTPKTGSHLITTGIFAYMRHPIYTGVFLTCVGFAIFQGSI
                     TRMVISILLLLFFDRKTVYEEKKLKLAFSEYEKYAAHTSKFFPFLKK"
     gene            23419..>24653
                     /locus_tag="PLM90_04720"
     CDS             23419..>24653
                     /locus_tag="PLM90_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HMZ88703.1"
                     /translation="MRLETEINKRRTFAIISHPDAGKTTLTEKFLLYGGAIQVAGAVK
                     SNKIKKSATSDFMEIERQRGISVATSVMSFEYQGINVNLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDCVKGVEEQTERLMEVCRMRKTPVIIFINKMDRDGKDPFDLLDELEDKLS
                     IKVRPLSWPINTGTHFKGVYSIYHHKINLFRPGSQRVEDDVITIDNLNDKTLDTQVGE
                     RDAEQLRHDIELIEGVYDAFDTNRYLKADVAPVFFGSAINNFGVQELLETFIQIAPVP
                     LGRDTDKRHVDADEEKFTGFIFKIHANIDPRHRDRIAFLRVCSGHFERNSFYHHVRLD
                     KNLRFNNPYAFLAASKDVVDEAWPGDVIGLYDTGNFKIGDTLTEGEDVMFRGIPSFSP
                     EIFKELVNMDPMKTKQLEKG"
BASE COUNT         7501 a         5116 c         5008 g         7028 t
ORIGIN      
        1 atattagctg gggcacacca attgcaatac ttgccggcga tgcaactgct tatgatgaca
       61 ccgatgttat tatcgattcg atgtatgaat acaaagtaaa aagaacgaat atttccggac
      121 ttgagggtga gggttattta ctcggtgcga ttacacttca tgctgtcgat tatcgcggaa
      181 cttgtttgct ggtaatggat actaccgtta cagatgcatt gacaaatgaa atcacaagat
      241 tgatggatga tatcagtggc gatggatgga gtgtttcaca attacatgtg gcaagagata
      301 tgtcgccgat agctgttcgt gaagctatta ttgctgctgc agaagcagat ccggagatgc
      361 aatcggtaat attaattggt cgcgttcctg ttttgtattc cggcaattta tatccagatg
      421 gtcaccccga tcatcaaggt gcctggcctg cagatggtat ttatggcgat atcaatgcag
      481 attataccga tgtcaccatc aataccacca cagcaggtcg acctgaaaat tggaatacac
      541 ccggtgacgg aaaatatgat caatcgatat ttaaatcaaa aatagaatta gcagtcggca
      601 gaattgacat gtacaatatg ccttcatttg cattagatga aaccggttta ttaaaaaggt
      661 atctcgacaa tgatcacaaa ttcagaacag gtatcacaca ttttgaacag cgggcattaa
      721 ttgatgataa tttcggggct tttgcgggag aagcatttgc aagcagtgga tggaggagct
      781 atgcacctct tgtaggaaat gataatctgc aggagttgga ttatttttct acgatgtacg
      841 acaacagtta catctgcagt tatggctgcg gaggtggctg gtttcaggga gctggtggtg
      901 tcggatctac aactgatttt acactcgata ccgtgaaaac agtatttaca tttttgtttg
      961 gaagttattt tggcgactgg gatgtaaatg acagtttttt aagggcacct ctagcgggag
     1021 gatatgcgct cactaatgca tgggcaggca gaccgcattg gagtttacat tacatgggac
     1081 tcggatatcc tataggatat gcatcgcgac aaacacaaaa taatcaatcg acttacacat
     1141 ccaatatttt ccccaaatgg gtgcacattg cgctcatggg cgaccctacc cttcgcatgc
     1201 aagtgatagc accggtttcg gaaatgcatt gcgaaatttc agatattcaa aatgccatcg
     1261 atatacattt tacagcatct gccgatcctg aagttatcgg gtattatgtg tatcgcgcag
     1321 caactaccta tggaaaatat gaaagaattt ctgatgatat tgtggccact tcaccctaca
     1381 ccgacctcac accaattgac ggtttgaatt attatcaggt aagacctgta atgctaacaa
     1441 caacacctag cggaacgttt tacaataccg gcattggctt gcgcgatagt attttgattg
     1501 atgttactga tgtaaataca ttggcacctt cgattgtaca actttatcca tcacctgcag
     1561 atacctatat tgtagtggtg acctcatccg atctcgtagg atcaactttc ataatatatg
     1621 atatttcagg aaaagctgtg cttacggtta aactattatc tgaagataca agtataaaaa
     1681 ctactaccct gcctgatggc gtctatacgc taaaattcag caacggtata accaggcaat
     1741 ttgtagtcag tcattaataa tccattcgtc ggaatatact tctttacgcg ggttccattt
     1801 atgtcaatcg aattcctcga tcagtatacc cattttaccc atttgataat cgcgcatagc
     1861 ctccagaatt tcggtttgcg aattcattac gaatggtccg tattgtatca ccggttcgtc
     1921 gatcggtttg cccgatagaa ttaagaaaat agtttcctga tcggcagtta attcaattcc
     1981 ggctccatct tggtgcaata caaccatatg caatgcaggc acaggtccgt atccggtcat
     2041 ggttaatccg cctttcacca catacaataa tgtttgatga tcggtcggga tttcaaattg
     2101 acgctttatt cctgccttca ttgttacatt tagcatcagc aggtccagtt ttgaatgcgc
     2161 aggccctttc accccttcaa aagttccggc aacaacatgt atgttacttc caacatcatc
     2221 aatggaaatt gacggaatat cattttcctg caaagcctga taattgggtt gatccatttt
     2281 tctggctgca ggcgtattca cccaaagctg cactatttcc tgcgaaccac ctttttctgc
     2341 aagttctttc gatgggcgtt cgctatgcat aatacccatt ccggcatcca tccattgcac
     2401 accaccggca tgcacaatag cagaatttcc cctgctgtcg cgatgatgca catcgccttt
     2461 gaaaataaag gtaaccggac tgaatccacg atgcggatgc ggccccactc ctacctcgcg
     2521 cggataagct ccggcagcaa ttttcgagct gtggtggtgc aatagcagaa agggatccac
     2581 ctggtctatg gcagctgaag gcaaaggttg acgcacgggc attccaccca tatccatcat
     2641 atcggcatat tgcaaagcac gaactgttct gagtttcatc atgctaacat aattgaaaaa
     2701 aacttatgaa agttcattat atgtgtacac atgttttttg aacatattaa agcaattgct
     2761 atattttatc atagattgtt gagattcctt aattttgcgc ccaatgcagg cgataggttt
     2821 ttatttatta ttaccgtttt tatatttctt ttccgtgcta ccaatgttca tattgcgaca
     2881 attgagcaat gtggcatttt ttatcatgta ttacattacc ggctaccgcc gtgaagtggt
     2941 gatgcggaat ttgggacatg cattccctga gaaaagtgag caggatttaa aaaagcttgc
     3001 gcgaggtttc tatttgtatt tgagtgacac gcttgtagaa acaataaaat catttactat
     3061 ctcagacgcc ggtttgcgga aatattgcgc cataaataat tatgatgttg tacaggcaga
     3121 gattgacaag ggtcgcaatg ttatgtttgt tttagggcat tatggcaatt gggaatgggg
     3181 aggcggagca ttggcaaaac gagcgcccat tccgctgaaa gccgcctata aaccgctttc
     3241 gaatccctat ttcgaaaagt tgatgttcca cacgcgcaca aggcttaata tccgattaat
     3301 tgccaagcgc aagatattgg cacatttgaa agatcatgcc gatgagcaaa ccttattggt
     3361 gatgattgcc gatcaatcgc caaatcctaa ggaacacttt tgggtgaaat tcctcaatca
     3421 ggatactgca gtggttacag gcatggaaaa aatcgcacgg caatataact atacggtaat
     3481 ttattctacc atcgccagtg ttggtcgcaa taaatatgaa attcggttca aaaaaatcac
     3541 cgacgacccg caatcattag caccggaagc attaacagct atgtttatgc acgaattgga
     3601 gcaggatatt tataagaacc ctgctccgta tctttggtcg catagacgtt ggaagctaaa
     3661 aaaaccgcag gcttaatcga ataatgtata tccaacagta aattgaaata agctcttgcg
     3721 ggtatctact tccagcatac ctttttctgt ttccacatat tccttaaact tgctcaggtt
     3781 gccttcatac ctagcatcga aggtgagttt gttgatgtcg aagttgatac cggtttgata
     3841 tccgacagtc atatctttca aactgaacaa gggatcgctc atagtgcttc tgaccgttgt
     3901 gaataccggt cctgccatca atttcactga tccgaaggct tgatacccaa gtaatatcgg
     3961 cacatcacat ttatctacag acatgctgaa taaatcgctc aaatccattt cattcacatt
     4021 ggcgtcagca agtgtcacaa gagattgatt ttcactgaat agaatttccg gttgcacgat
     4081 ccacctgccg aaagtagctc ttgcaaatcc gccaaatacc aatgccgatc ctttttgact
     4141 ttctacatca gccacatcaa caccatcgag actcaccaca tttagaatta cagattgtgc
     4201 atagttgaca ccacctttca ctccgagttc atattcctgt gcctgcagat ccactgcagc
     4261 aacacaaaac aggatcagca ttattctttt cataaccttc gatttacagg taggtaaacg
     4321 aaaactgtgc cacaaagcac atcgctcatg acgactactt gtaaataggc tatttttgga
     4381 cattaaaatc aaataatatg tctgcaactc cggaatggaa catacgactg gaacccagaa
     4441 atatcaagta tttgagtggt ccgcggtcta gatggcgcga attatggtgg gcattttcgg
     4501 tatttaccga atttatcaaa ggatttagag ccttgcactt tataggcccc tgtattaccg
     4561 tttttggatc tgccagattt aagcctgatc atcaatatta caagcaagcc tatgatatcg
     4621 gcaagaaaat gagtgcgttg ggattaacag tgctgacagg tggtggtccg ggaattatgg
     4681 aagcagccaa caaaggcgca tttgaaaatc aggggcgttc cgtaggctgc aatattgtat
     4741 tgccgcagga acaaaaacct aatccgtaca tgcataaatg ggtgactatc aagtactttt
     4801 tcgttagaaa ggtcttattg gttaaataca gctacggatt tgtggtaatg ccgggaggag
     4861 tcggaaccat ggatgaattt tttgagacat tgaccttgat acagacagct actattcata
     4921 atttccctgt ggtgatgatc gggaaggatt attacaaaga catttgggaa atgctgcaaa
     4981 agatgacaca ggcaggcacc gtatctccgg aggacttgaa tctggtgctt tttaccgata
     5041 atgcgcaaga ggctgtaaac cacattgatt tttacatcaa aaagcattat tcctttaaaa
     5101 aagttaaacc tcgcaaactt cttttagaga gatttgttta gaagtaactt tgtttggcaa
     5161 tgcgcattcc tgaaaagaca atgatgtggc ttaaacgctt tggttgggcg ggatttttct
     5221 tcttccttat caagggcatc gtttggctca ttgcaggtgc tgccattatt gaatggtttc
     5281 agaaaacctt ttaagcgctc tcctaccctg ttcatcccgg ttttactccc gttcatcacc
     5341 cttcattaaa ttaatatatg cgtgcttccg tgctttgtgg cagaaatcaa aacatatgaa
     5401 acaaataacg ctattcttgt tttgcctgac catagggact tttgcccggg cacaacagct
     5461 cacacaaacg attcgcggac aggtgacgga taatcaatcg aaagccacgc ttccgggagt
     5521 gaatatcatg gttttaaaca gcgaccctat attaggtact acatcagatc tcgatggcta
     5581 tttcgcgatc gaacaagtgc caattggacg ggtaagtata caagccacat ttattggtta
     5641 tgaaccattt attgtaaata atcttgaact tacgtcaggc aaggagttga tgctcaattt
     5701 cagcatgtcg gaacaagtaa tcactatgaa tgaagtagtt gttcgtgcga aagatgacaa
     5761 aatggaaacg aataacgaga tgaccactgt cagcgcgcga caattcacta ttgaagaaag
     5821 tatgcgctat gccggagcga gaaatgatgt gagtcgcatg gcacagaatt tcgccggtgt
     5881 gcgcggatct aatgatgccg taaatgatat tgttattcgc ggtaactcac cggttggtgt
     5941 attatggcgt cttgagggga ttgatatccc aaatccaaac catttcggcg acctcggttc
     6001 cacaggtggt ccggtttcga tgctgaataa taatattctg gctaacagcg acttcttaac
     6061 aggcgctttt ccggcagagt atggcaatgg tatcagcggt gtttttgatt tgaaaatgcg
     6121 caatggcaat tacaaaaaac atgaattttt aggtcaggtt gggttgaatg gatttgaatt
     6181 tggtgctgaa ggccctttaa gtaaggatag caaatcttca taccttatga attatcgcta
     6241 ctctacactc ggattgatga gtgctatcgg cataaatttc ggaacaggaa ctgccattcc
     6301 ttattaccag gatattacct tccgcgtgaa tatgcccact agaaaagcag gaacctttgc
     6361 tttgtttggg cttggcggcg tgagttcgat tgatttaatt tctagttcaa cagaagattc
     6421 cgttgacaat ttatataata atgatctcga catttatgat aaatcaaaaa ttggcatggt
     6481 tggatttacg catcaatatc tgatcaatga taaatcatat accagattta cactttccac
     6541 ctcatccatt gaaaatactg atattattga ttctatatcg agcgaaaatt ttgaaccgat
     6601 taattactac aatcaaaatt tcacccagca taaggcatca gccagtctga tgtataaaat
     6661 taagctcaac agcagacaaa atctgcaggc aggagctagt gcagatatgt tgtttgcaga
     6721 tttaaatgac agtatttatc gcaatacatt agatcgctgg atcaccatta cttcatacaa
     6781 tggtaatacc atgttattgc agggttatgt gcaataccag ataaggctta cagaaaacct
     6841 gactgcaacc gcaggtgttc atgaccaatt tcttgcactt aatggcagta attccgttga
     6901 accaagggca ggtatgaaat acaccattaa tgaaaaaaat gccatcagct tcggttatgg
     6961 attgcacagt atgatggcgc ctattagtgt atatttcaaa acagttgaaa ccactcccgg
     7021 aaattacatc acaccaaacg acagcattga ttttattaaa gcacatcact ttgttcttgg
     7081 ttacgacaga caattatcaa gcaccatgcg attcaggtct gaaatatact atcaacatct
     7141 atatgatgta ttgatcgatc gcgacagttc ttcttattct gtattaaata ccggcagtat
     7201 gacatttggt tcaccagatt acctcgtaaa taaagggaca ggtcgcaatt atggtgcaga
     7261 ggttacgtta gaaaagtttt tggataaggg attatatttc cttgtcaccg gttccatata
     7321 taaatcggaa tatctaggca gcgatgatat tgaacgtgaa actgcattct cgggaaatta
     7381 tgttgccaat gtggtaggtg gaaaagaatt cgccatactg gccggaagag caggtgctaa
     7441 aacgaaaaaa tcggtggtgt ttgacaccaa actaaccatg gccggtggtc agcgatacac
     7501 gccaatagat cttactgctt ctatagatga gggtaaaacc gtttacgatt gggataaggc
     7561 atttgaagca caattcgacg attatttccg ctgggatgta aggctagcat ttaaaatgga
     7621 tggcaaacat gcatcgcagg aatgggctat tgatgtgcag aatgttacaa atgaacaaaa
     7681 tcctttatat caaagatata atccggtttc acaaacagta aatactattt atcaattagg
     7741 tatctttccg gttccgcaat atcgcatcac cttttgagca aacaatctta tataccattc
     7801 agaaaggcgc tggcaggcac attagttgtg tcgctggtgc ctttaattta tcagatactg
     7861 gtttactaca tcgtaggcac gcccaaatgt cctgaattaa ccttttggga aaatgccatg
     7921 tggaccgctg tgttctcctt gctcgtaacc tttgcgttgt ttatagtaaa catctacatc
     7981 aatacaaaag gtcatgaatt actggaggaa aaagtaaatg atggcatcag aattttcgtg
     8041 acggtattcg tttcgctact tgcctcgaat attgtcattt ttgtgatgtg gaaattgttt
     8101 aatgacctga tattacatct tccagaagat ggagaaacgg cgcgtgtttt ctctaatcaa
     8161 atattagcaa cggtactggt tatcattgtc gatttgatca ttgaggtgga tatttatgtc
     8221 aagaaactta aaatcagtat ggaaacacaa gcgcgcctgg aaaaagaatt cattcgcgca
     8281 caactggaga gcttaaagac gcaaattagt ccgcactttc tatttaacag cttcaatgcc
     8341 ttacaatcat taatcgacca atctcctgta caagcaaagt cttttatcca ggaactggca
     8401 aaggtttatc gatatgtgct ggataaaaaa gacgaaatgg tagtgcctgt ttccgatgaa
     8461 gttgccttta tccgcagttt tctttattta aatcatattc gctttggcga cagcttatcc
     8521 gctgatatag atttatcggc acaggaaatg gacgggttta tcccgccttt aacgctgcaa
     8581 ttacttgtag aaaatgccat taaacacaat atcatatcac aagcgcgacc attgcatatc
     8641 agtgtttttc gcgatgcggg aaatatcatc atcaaaaaca acctgcaact gcgagaggac
     8701 actccttctg cctcaggcat cggccagtcg aatttggtgg cacgatataa tttgataagc
     8761 gacagaacac cttcattcaa cattgtagac gatgcttata ttgcaaaggt tccggtatta
     8821 gaacaagaat tataaggtca tgcacgtatt aattattgaa gacgaatccc ttgctgcaaa
     8881 ttggttgcga acacaaattg agcaatctac aaaagatatg actgtagtag ccgtactgga
     8941 cagcgtgtct gcatctgtag attggcttca aaaaaaccag gtcgatttaa ttttttcaga
     9001 tattcattta gcagatggta taagtttttc catttttgaa cgcatagcta tttcaacccc
     9061 catcattttc actaccgctt atgaccatta tgccattcgc gcgtttcagg tgaatagtat
     9121 cgattatttg ttaaaaccta tagatacaga agccctttcg gcgtcaatag aaaaatatcg
     9181 cagcctgcat accaacattc aaccggattg gaaaatgctg gtaagcggcc tgcaaccaaa
     9241 aaacaattat caggagcgtt ttcttgtcag caccggacaa aaattgaaat cacttcccat
     9301 tatcgatatc gcctatttct ttgcagataa caagttgaat tttgtttgtg acacgaacgg
     9361 tcgcacctat attctggatc aaacactgga caaacttgag caggttgtag atcccggaac
     9421 attcttccgc atcaacaggc agtttatagt gtcatttggc gctattcaag atatgttgca
     9481 ctactcaaag tcacgcgtaa agctcacttt gcatccctca actgagcacg acactatagt
     9541 ttctgctgaa aggtcagccg catttaaggc ctggcttaat cgttaagagc ctttgtttta
     9601 gcatgtatgc gggcaattac actaactttg tttcatgaaa tattggatgc tcctttcggc
     9661 cattgcccta gcctcatgtt cagctacttt agaaaatgaa cctatcacac acacaaattg
     9721 gcctacaaga gaagaactgt tccagaattt tcgaaaccag gacactgcta taatagtgta
     9781 tggaaaggaa gattataaga tttctgattt attggtgaat ggcgtagcgg cgctggctcc
     9841 gaaagatgat cgcttcacgg tgattacacg cagagacgat aaactgacag aatttgaggc
     9901 tgctaattat ccgttatata tcatcggcac cagaaacaat cttttgataa aacgcgttag
     9961 cgaggcaatt cctgtgaaag tttttgaggg tggatttaat ttcaatggaa cggattatat
    10021 ggcgcccaat gatgtgatca aactttcatt ttatcccaat gtattcaacc cgaaaattcc
    10081 atttaccgtt atcacaggca atcacgaaga agccatagtg caatatgtgc agcaggagat
    10141 gaatggcgag tggggatatt tcttttggga taattggggt tatcaggtgt accataatgg
    10201 cagacgaaca atattgggta atttcagtca agacagtgct tcgctatgga gtattgataa
    10261 aaagcaacat tgggaatttg attttgaagg taaaagttta aaaacctcgg gccaggttca
    10321 ttataccgga cacaatgatc caaacgttac caacttagac aattgggacg ctgtagcaca
    10381 agatgacctc actcgtatat cggaattctg cggaaaaaag ataagtgcca gattagaggt
    10441 gcacctctac cccactccgg aaacaaaaac tttgttgatg agtgatccgg atcagtcgac
    10501 aattgattat gccgcttatg ccatacatac tgtaatttcc ggtgaatatg aaaaccaccg
    10561 cgacgagaaa ttaaatatgc ttgcactgcg caacatgatg gggaatgccg gatgtgttgc
    10621 atatgaagaa ggactatccg ttttatatac cactaactgg caaaatgagg atataaggtt
    10681 gctggcatta aaacttcgcc aggcaaatgc acttccatcg ttgacacttt tattacataa
    10741 tgatgcattt gaaggcgaat cggattatct gatgcatatt gccgctgcca gttatgtcga
    10801 ttttatcatc aatagtcagg gaaaggaaag cttccttgct caatacagtc aaaagccggt
    10861 aattactgct gaattagaaa ctgcctggta tcaatatata gatgatttcg gcaagcgtat
    10921 tcgaattgct ccgaagccaa aacccgcact accggagcat cttaagggct ttaattttgc
    10981 tcatgaagga tatcagatat acaatggcta tatgggttca gaagctgaac attctatcgc
    11041 caaactaaaa gaaattggtg caaatactat tgctattatt ccatatagcg gatttcaatc
    11101 gatggataaa ccttatcgct ttcgctacac taatggaccc ggcgcggaaa atgatgcagc
    11161 agttgtgcat gcaggctatg ttgctcatca gcaaggaatg gttaccatgt tgaaaccgca
    11221 attatggtct tggttaggat ggactggaga tattaaaatg cagaatgctg ctgattggga
    11281 tttgtttttc gaatattatg aagaatggat catgcattat gctttgttag ctgaactata
    11341 tgattatgat attttgtgca taggcaatga atttcaagcc gcaacgcttt ctgagcatgc
    11401 aagatgggat gccttgtttg aaaaagttcg actagtatat catggaaata tcacctatgc
    11461 agcaaactgg ggttctgaat ttgaacaagc cggattttgg gatatgttgg atttcatttc
    11521 agtgaattgc tactatccgc taagcgaaaa aacttcacct actgatgccg aattgttagc
    11581 tgcttttgag cacaatttag atattataga aaaggtgcaa aagaaatata ataaaccggt
    11641 aatcttcact gaaatcggat ttaaaagtat tgcttcccca tggatagcac cacacaaaga
    11701 tgccgacgaa caagcatatg acgaaacatc acaagatcga tgctaccaag taatgtttaa
    11761 agcaatggca gacgaaccct ggatcaaagg aacctatatt tggaaatggc caagctatat
    11821 ggatttctct aaagaataca gcaaggattt caatccctgc aataagctgg cagaaaaaac
    11881 aatctcagaa aattacaaaa agcaataatg tcgtattaat aatcagcaac gcacgcacct
    11941 ttatacaaga tcatgtaatc tacaatagat tgaatggctc ctgtctttag atcgcgcgtt
    12001 aaactcttgt catttattac ggaagccgtg atgcgccata cctgacagaa atcgtaatcc
    12061 attgcttttt gtgcctgcac catacccaga aataaatgcg tggtgaaact tgtgctgttt
    12121 ttatcagccg ccatctgaag aacattgatt gcgtcatcta ccacaaaata attatccatt
    12181 gccataaagt atccgaggca aaacaattca tcaccgctta atgatgacat ggatagttgc
    12241 tgcgtggtag tattatgtgc ttttgcaaga tatgttttaa ataaatcggc attctgtgtg
    12301 ccatcgtaat tccacccaat ggcattgatc acggcagctt tcacatcgat cagattatcc
    12361 ggatcgagca aatacgctgc tatctcatcg tttaatccgc gcgttgtttc agcgattccc
    12421 actatgggat attcgtaata aaatgttgca atataggttg aggtaatcgg cgaatctgct
    12481 tttactgtta atgacattgt cagcactaaa gccaaaacag catacttaat ccttttaatc
    12541 atgttgtgta gtttgaggtc caaattgttc gggatgttca tacatgtaat aactcaatgg
    12601 aatggcaata gccaatgcaa ttaccaatgg aataattacc cttctgtgtt tataaggttg
    12661 atgttttcca aaaatgcgcg accataaaat ttcagataat agcaatccgc aggctatggg
    12721 aatcagtatg cctaaaaaag tgcctttggc ataataccag gtaacgtaac cgctgatggc
    12781 agaaacggca aaactcagca atacgaccag ccagccattt ttcttttgca tgcgcttcat
    12841 gttcatggca aaaagtatac caccaaatgc aggagaaaag aatatcgaaa aggcaagaat
    12901 cgcagtttgg gaataaagtt cgggtaactc gataggtatt ggtcgctctt catcaatagc
    12961 ctggagtatt tcttccggtt cgatgcgacc ggcaatttca gcgcgcaagg cctcagcatt
    13021 taacacggtc aggccacgtt tttcggcaac atcgagcata gcagtgagca tatcatctac
    13081 atacttctca tggtgtgcca gggcctccag caggtcttca tcggtcattc gggccgcaac
    13141 gagcacatat gtattatccg gcataatttt taatacctaa ttttgtcatt atgaacaaga
    13201 gtgtaaagtt attgctaatt atctgctttt tcacagccct gccggcattt gcccaaacac
    13261 aactcaatac caagggactg ttcacctttg tcagggtgct tcccatccca tcagattttg
    13321 agatcggacc cgggcaaatc aataaagatg gcagttcatt ctttgtgggc cttaccgatg
    13381 gcgacctgga aaatatggaa gacctgcatt cagatgtata tcgcgtgaat gttacttctg
    13441 atgtaaatgc aattccaatt accggtttag agatccctaa tgccatcgac agccttcgtt
    13501 tttttcaggc ttccgcttca gataacgatg cctatatcgt ttttgttgta aatgcctggg
    13561 caggatggaa tgacaatgaa ctggccattg tgaaacgaca accatcagga gcttttggcg
    13621 gaatgcgcat gcttaccgag gtaaataaag aacatttttc tgatgcttat ccgtggcttt
    13681 ccggtgatgc aaatcgtctg tatttttcac aggattttaa attgatgtac agcgaacgca
    13741 gtagtcccga ttcactattt tcttccccca aacctgtcga ttttgttggt gatgtgcaat
    13801 tggaaattgt aagtgcatgg cttccacctg atgaaaaatc aatattctta ctcgcaaaca
    13861 atcgcattta taaatcaacg cgtaaatcaa ctactgagcc tttctcattt ccctccttgt
    13921 ggacggatga gtttaaagat ttttattttg tagcaggatt gagttttgca ccggataaaa
    13981 aaacgatgta tatttatcat tctgacgaaa catcacaaca tattttacaa tatcagttaa
    14041 gcaaaggaaa ggcctggtaa gactacaaaa catatgttgc ctgttggtgt tgccaataat
    14101 atggcaatcc tgctcgaaaa caatcgagcc gcccgattac agcaatttca ttcacgttca
    14161 aaaaaagggg tctttcgaaa tttatttgcc ccctactcta tatcctgata aatctatcag
    14221 cgaaattgca caaatggcat ataccgattc gctgcatcat agttttttaa tggtgctgcg
    14281 cgatgtcact cccgatcttg aagaagatag tatttttatt acacatcaac gctatcatgt
    14341 atatgcttgt gccggaattg aaagtgctat gctaaatgct gataaagatg atactgatag
    14401 tgtcatgacc ggtgggaact tagtatatac tacgccgatt gaaggagaat tcggtgggca
    14461 tgaagtgcat tatcgtgtat ccactataga aacagaatat tatttctacc aaatactcac
    14521 atggcatctc gcagacaatg acgcgatgga aaagcaatta tatgatgcta tgttaagttt
    14581 tcgacctttc aggtgataag gtcttatctt tgcctgatga tcaacttcaa aaacacagga
    14641 ttcgcagtga ttgcaatttt gatgacggcg agtttcattt catgtggtga tgaaaatcaa
    14701 gctgatgaaa aaacacctgc accattgaat cgcagcatgt tctatggaaa gataacttat
    14761 acttccacaa cttcatcacc tgattctgtt ttccttgcaa ccatgtcgac tttcagtccg
    14821 cacgctgttg acatctactt cgggaaatct gcatttcgca tggtagaaca cggtggctta
    14881 tcaaatggaa atattctggt actacccgaa tccggcgaag catggcaact cgacagcaca
    14941 aaaatgatcg catatttagg tgaatattca gaccttggag acccaagtga tgcgcttaaa
    15001 gatctgatgc ccgatcattt cgctcctact gtaaaagcta caggcaaaac cgccaatatt
    15061 cttggcattc catgcacgga atattatatc gttcgttcag gtatcattcc gaaggaagac
    15121 agcgctacaa tcatggtagc caatactatt aatttccctt catcacgcta tgatgtgcaa
    15181 actgaaatca atcatgctgc cgttcctgca ccgctttttc ttggttatga agatggcgct
    15241 atcatgcaaa tgacagttgt aaacaagcga tatacgcgca aatttgaaat aacagcacta
    15301 caggaaaatt atttccccga aggtatattt gatatcccgg caaattttca gaaaaaatga
    15361 acagactcaa attgctgaca ggtagttttt tccttgcaca aaccttaatg gcacagtctg
    15421 ttcaggtaga ttctgtaact gcaccttcaa gcttccgtgg catggcagtt gtaagcgatg
    15481 ctgaaatttg gataagtggt agtaaaggat atatcggaca tagtatcgac agtggtaaaa
    15541 cctatacgtt tcaccaaatt cccaaatacg aaaatgctga aatccgcgat atcgaatttt
    15601 taaatgatgg cgctttggtt gctatgagca gcactcagcc agccgctatt ctaaggtcta
    15661 cagataaggg agctacctgg aaggaagtat ttcgtcgcga tacatcagca tatttccttg
    15721 atgccataga cttctccgga aaacaaggca cttgtcttgg cgatcctatc aacggtcatt
    15781 ttctcattct tcataccagt aattatggta aaacctggac agagacttat acaaatatcg
    15841 caaccgatag cctcgcagct tttgcagcaa gtggatcaac tttgcaatat ttaagtacca
    15901 atgaaattgt ttttggcacc ggaggaaaag aagccctgtt attttattca aaggatgcag
    15961 gaaaaacatg ggaaacaact gaaatgctga tgaattccgg aaaggaaagc gcaggtatct
    16021 tttcggtggc cgttattgac tcaaaaaata tcgtttgcgc cggcggcgat taccttgacc
    16081 cgacaaacac agattacaat taccttcttg tctatatcat agaaggcgtt tgggactacc
    16141 cgattccggt aagtgaaata aataaagaaa gctctggtta ttattcctgt attgatgtta
    16201 ttacaaataa taatatcaca acattttttg catgtggaac aggaggtgta gacgtttgga
    16261 gaagtactaa ttttaaaatt aaggtacttt cgatgtcagg atttaatgtt ttacagattt
    16321 cgccctcggg gaaatatgta tttctggcag gtaataaaat tgtagggaag atggatattt
    16381 gaggaataac tcctacacca taaacaacaa atacaaggca taaaaagaag ccgcaacgcc
    16441 aagtacgcca ataatgataa aattaacgat ggcaaatttc tcgcctttat atttttttgg
    16501 ttcagccttt attttcttcc aacctttaga cgtcagtata gcacttgcta tcagtaataa
    16561 tgcaccaatt gccaataaaa tcggagccgc aaacattccg gctgcagcaa ctcctgctcc
    16621 tgcaacggct gctcctgtgc ccgctaaaac cattggctcc aaaacaggtt cctgctcctc
    16681 tttctttccg gtatcctcct tcacaacagc gtctactgca tcaatgaaag tattatctgc
    16741 attgatcacc gcacccggtg tcgacatatc tgtttcaaca taaacaacag tattacttcc
    16801 ggctgaataa tcagcagatt tatctattga agagggttga ttcccaacta actcttcagc
    16861 atataatccg gaaaacccaa tgcaacaaat tgcgcttaac gcaacgacat tttttaaacg
    16921 aatcatttga aacataacgg tcaaaaatag ggattattat cttgatgcca ctgccacctg
    16981 ttgttgaaac aaacctagta cattggacca ctgcagcagg tataagcccg aaggcagcga
    17041 ttccggcaag gtgataagca cttctccccc attggtctcc tcgaaaaaaa cttccttacc
    17101 acccaaatcc atcaatcgaa ttatcgtggg catttcaggt tgatttgttg aaatatgtat
    17161 ttcatgatta gcaggattag gatataccat agattctgcc tcatccatca ttctaccacc
    17221 tactaacacc gtcgccacaa cacttgctgt gcaattatgt gcatcttttg tcatgatagt
    17281 atacgtgccg gcacttaatc cggaaaattt cttcgccgtt tgccaggtca aaccaccatt
    17341 aatagaatat tgtttggagc catatccacc tgttgactgc acagtgatct ttccattgtg
    17401 tgcaccggca acagtttcat tcactattga agtaactgtc agggtaagca atgcatcctc
    17461 actgattacc acatttccca ttacaaaggc accacatgca tcggtgactt ttactttata
    17521 agttgcagca cacaagtcac ttaaaaatcc atcaaaaggt atccaggcac cggcaccgat
    17581 tttatattga taagtatagg gtgcacatcc ataaataccg tccaattcta aagaaccatc
    17641 gcagctgcca tgacagattg ttggaatgga attaacaaga tatgataaag gagcagcaat
    17701 ttgttcatca gcacccattt ctattctgcc tcccgtaagt ctggttgcca agtccatatc
    17761 aaattcatca acaggaatgt aacttggatt tcctgcattg atacatggcg atgttacttg
    17821 aagatgaaaa tccgaaatgg caggatttac gaataatgga tcagcaagtg aaaaatgtgc
    17881 attgtcaccc attgttgaaa gcagcgcttc ataacctgta attaaggtaa aatcaacaaa
    17941 gcccttcatt gccatcggtc cgcccgaata ataataattg ttgtagtcca gatttacttc
    18001 gtaggtagaa tcaatagttt cggcattgat catccatggg gtagttgatg tgaagaaaat
    18061 attattttca acaatgcaat tcgttgcata ttgtatccat aattgaccaa atcctgtatt
    18121 caggaaatca ttgtcatata ccgtatttcc atagaaagca cagttggtaa ccattcccgt
    18181 agccggataa tcatatcctc caaaacctag tccggatttt tgattgcgat aaataagatt
    18241 attccgaact gtgatgccac tacaaacaaa tcccatattc tcacaaccta tttctaggcc
    18301 tatatcacta tcggtgatag aattattttc aactacaata tttttaccgc catctacata
    18361 tacacctgcc gaaaatccgc caccataaat agaattcgca gccgttacag catttcttct
    18421 gcaaaccccg ttacgggcct gatttaccgc atcattggta tttacccaat cttcaccacc
    18481 aatcatgacg atgccgatat tattgatatc cgtcaataca ttatctgcaa ttgtaaatcc
    18541 atcaatatta ccattcactg caattgcctc gcttggtgca ggatccaggt tagtgagtac
    18601 attattggtg atgacaatat tggtataggc ggtggtagtt cctgaaccaa caaataatac
    18661 acccacggca ttatcaccaa tcaggtcgtg catattattt ccggagatca aaaaatcatc
    18721 gccaaatcct tcgcatagaa tggcatagct ctcattgcaa ttggtattgt aaaattcgca
    18781 attggtgatt tcaatatgac tgcaattgcc atacacatta atgacgggct ggtaatttcc
    18841 gttgtgattg gtaaaacgca atccatctat tttgatgtaa tttttatttt cgatgtacat
    18901 caaagcctga ttattgccca cagtgcttcc atccagaatt accacatcat cgttataatt
    18961 ttgcaatgta atatattgtc caaccacacc gccatcattc caggtcaatt taccggtgta
    19021 ggttcctgcc attacgttga tcacatcgcc atcattagcc tggttaagtg caaattgaat
    19081 ggttttccag ggagaggcaa tgccgatgcc tgaattgaga ttatttcctg ccggtgaaac
    19141 ataatagttg gcgccatcta aatttattag tgccatggag atcattatac tcagaataat
    19201 attcaatcgc atggttctaa ggtaatgtgg actatcgaca taaaaaaacc cggactttta
    19261 cctccgggtc tcttttcata ctactgcacg cactcaaatt cctgacagat aacgacttaa
    19321 ttcatccaca ctgctgctaa accaaaattc cttgttcacg atgaagtaat tctctgtatc
    19381 tactgtttta taatatgcgg ctttcgctaa ttccagcctg gtgtcttcaa aactcatggc
    19441 atgcatcatt tcggcaacct gattggcagt aaaccaattg ctattcaaag cctgtttggc
    19501 aatttgcagt cttccactct caaaccactg gtcttcaata acatccatga gttgtgtaaa
    19561 gctgtaacca gccatcggca atggtttcgg cggcaaaggc ggagtaccgc aggttggata
    19621 agtattcggc gggtaataag tctgaggctc gggaacatat accggaatgc ttttcaccga
    19681 cttgatataa aatcgccctg cattgtccag aacgcctgtt accagtgaat ttgcgtcaat
    19741 gtagatgtcg ccgctataga tcaacttctg cgctcctgcg tggccttgtt gacctgctgc
    19801 aaacaaatca acatggtgca taccggctgt catcgttgcc atggaaaatc tcttaaccgg
    19861 attggggtaa taccgaccat caagctgcac agaaaagtaa taactaccct gcgacttgat
    19921 ctctaaggtt gaattggcta ccggcaccca accggcaggc ggattggcac ttgcaatgct
    19981 gaatggtacc agaatggcaa tcaatagttt gtgtacgata gttttcataa aaacctcctt
    20041 tttcttaaca tatattccac tatcgtgcca atttgataca tttatgataa ttttcttgac
    20101 aactactgat agaaagccct aatacggggc gcttggcgta tttttacccc gtgatcaaaa
    20161 aggaaaccat agaccgcatt aatgataccg cccgcataga cgaggtggta ggcgattttg
    20221 tgcgtttgaa gaagcgtggt gtcaatttga tcggtctttg ccctttccat aatgagaaaa
    20281 caccttcttt caacgtatcg gtgcccaggg gtatctataa atgctttggc tgtggtaaag
    20341 ggggtaatgc tgtatcgttt gttatggaac acgagaaatt cacctatcgt gaagcattgc
    20401 tctatctggc gaacaaatac aatatcgagg tagaagaaac ggagcgaacc aatgaagaaa
    20461 ttcttgaaga taatcgtcgc gaaagtctat acatcatcaa tgcatttgca cagaattatt
    20521 tttcagagca actgctgaat tcagaagaag gaaataatat cgggctgagc tattttgaag
    20581 aacgcggact ccgcagagaa acgatagaaa cattcaaact cggttattgc ccttccgatg
    20641 gcgactcatt tacgcaatca gcgcatcaag ccggatatca aaatgaattg ttgcaacatc
    20701 ttggattgac tacagcctat ggaaaagatt ttttccgtgg cagggtgatg ttcactattc
    20761 aaagtgtgag tggtaaaata ttgggatttg gcggaagaac acttagtgcc gataaaaagc
    20821 agccaaaata tatcaactct ccggaaacgg atatttactt taaaagtaaa attgtttacg
    20881 gcatttatca ggcaagaaaa gccattgctc agcacgatgt ttgttttttg gtagagggat
    20941 atatggatgt gatatcactg catcaaggtg gtattgaaaa tgtggtagcc tcatcaggaa
    21001 cagcacttac acaggatcag gtgcgactga tcaagcgcta cacacaaaat ctgattatat
    21061 tatatgatgg cgatgcagcg ggtgtaaaag ctgcgctgcg cggattagat attgcgctgg
    21121 aagaaggcat gaatgtaaag gtcgtgttgc ttccagctga agatgacccg gatacattgg
    21181 ttcaaaaatt aggtggcgac ggatttaaca aatatgtaga ggcacagaaa aaggacctca
    21241 tacatcttaa aacagatctc ttccttgaag aagcaaaagg tgatcctgta aaagttgccg
    21301 gcatcatcaa ggatattgta gcctcgatag caaaaattcc tgatgccatt acgcgcagtc
    21361 tgtatgtaaa acaaacggct aaaatgctgg aggttgaaga gcagatactg attaccgaaa
    21421 ctaataaatt actgcgcaaa gaggcaacta aaaaagttac tcctgaagac aaagccactg
    21481 ttgaagctct tgattataca ccacctgcaa aagaacagcc actcttcact tttcatctcg
    21541 acactccgca ggaacgagat atagtgcgcc tgttacttga aaacagcaat tatgaaatag
    21601 aaggtgaaaa tgccatcgcc agaatattgc gcaacctcag tgatgttgag atcgatcatc
    21661 cggaatacaa tatgatcatc gaagaatatc gcaatgcgtt tgcacaaaac gaatttctta
    21721 cacaagccca ttttttcaat caccctgatg aggccataag aaacctgacc atagatttat
    21781 tgcaatcgcc ttacgagttg agcgataact ggacgaaaat gcatgatgtg cctattacgg
    21841 acaaacagtt tttggtgcga aaggacattg tgaaatcaat ttcgctgctc aagctcaaaa
    21901 agatcattcg tatgaagcag gacgttgata atcgcattaa agaattgcaa cacgatacat
    21961 ctgaaaacgg cattcaggaa accatgctgt gtcaaaaaga gagcattcaa ttgcaacagt
    22021 ttatccgcaa actatccact gataccggca ctgtcgtttt gcccggcatc ctctgaaatg
    22081 cccattgctt ctttgcgcat tttctcactc aattccgttc caagaaaatt atctatgata
    22141 tggtgcaccc aatataatac cggtgtaagt acaatagctg caagaatttt ataaaagtaa
    22201 tttacggttg ccactgcaaa gaaaaatggc aaagtccaat gttgcggacc tagcacgaaa
    22261 gcaatataca acacgatcaa actgtcaaat atctgtgaga tagcagttga acctgttgca
    22321 cgcaaccaaa tacgtcgctc accggtaaaa gccttgatgc gatgaaaaat aaaaacatct
    22381 acaatctgac tgaataaaaa ggcagcaata ctggcgccaa tgatccacat tccctgcccg
    22441 aaaattgcag cataagccgc ctgcatatcc ggaacaccgg agtcaacaaa acttcccggc
    22501 caaaaggcag caggtttcaa actaattccg gcatatacaa ttgcgaaagc atatgcaata
    22561 caaattacag ccagataact caatcggcgc acacctttta caccaaaata ttcattgatt
    22621 atatcagtca tgataaaaac aaaaggccat aatatcacac cgactgtaaa attcaatgag
    22681 cctgcctgcc catataaatt ccagttaaat ggctttaacc cgagactatc ttccaaagca
    22741 aaaaccttca ctccgataaa ttcagctatg atggcgttga ccacaaaaaa cacacctagc
    22801 acaagcaaca agatagcagg cttgctagcg aagggattgt gatgatgttt atccatatgt
    22861 tatttcttta aaaaaggaaa aaattttgat gtatgcgctg catatttttc gtattcacta
    22921 aaagcaagtt taagtttttt ctcttcgtaa actgtcttcc tgtcgaaaaa tagcaacaag
    22981 agaatactaa tgaccatgcg cgttatgcta ccctgaaaaa tggcgaatcc tacacaggtt
    23041 aaaaacacgc ctgtatagat cggatgacgc atgtaagcaa atatgcctgt tgtaatcagg
    23101 tgcgacccgg ttttgggagt cggatacatg gtaagatttc tgcgcaattg caaaattggc
    23161 caccaaaaga gtatgagccc aatcacccca atcaatagtc cgactaatct acctgcaatg
    23221 cccggttgaa aggaatactg ccacggttcc caaatgtaaa cagcaaaaaa aaccaactga
    23281 ataaatacaa gtatcctgtc ctttaccata agctgccctg aaacaactga aacatacaac
    23341 tttttgctga aacatcgtat tttatggtgt gagtaagccc ttattggtat ctttgcagcc
    23401 gcttaagagg gcagtatcat gcgattagag acggaaatta acaaaagaag aacctttgcc
    23461 attatcagtc atccggatgc cggaaaaact acacttacgg aaaaatttct gttatacggt
    23521 ggagctattc aggttgcagg tgcagtaaaa agcaataaga ttaagaaatc tgctacgagc
    23581 gacttcatgg aaattgaacg tcaacgtggt atctccgtgg ctacctcagt gatgagtttt
    23641 gaataccagg gcatcaacgt caatctcctg gatactcccg gtcacaaaga ctttgctgaa
    23701 gacacttatc gcacactcac ggctgtcgac agtgttatcc tggtaatcga ttgcgtaaaa
    23761 ggtgttgaag agcaaactga acgcttgatg gaggtttgcc gtatgcgcaa aacgccggta
    23821 attattttca tcaacaaaat ggaccgcgac ggaaaagatc cttttgatct gttagatgaa
    23881 ttggaagata aactaagtat taaagttcgt cctctctcct ggcctattaa caccggaaca
    23941 cattttaaag gcgtttattc tatttatcac cacaaaataa atcttttccg tccgggttct
    24001 caacgcgtag aagatgatgt aatcaccatt gataatctca acgacaaaac acttgacacg
    24061 caagttggcg aacgcgatgc agaacaatta cgtcacgata tcgaattgat tgaaggggtg
    24121 tatgatgctt ttgatacaaa tcgatattta aaagcagatg ttgcgccggt attttttggt
    24181 tctgccatca ataattttgg tgtacaggaa ttattggaaa catttattca aatcgcaccg
    24241 gttccactgg gaagagatac ggataaacgt catgttgatg ccgatgaaga aaaattcacc
    24301 ggttttattt tcaagataca tgcgaatata gaccctcgac atcgagacag aatcgcattt
    24361 ttacgcgtgt gcagcggtca cttcgaacgc aatagtttct atcatcacgt gcgcctcgat
    24421 aaaaatttgc gcttcaacaa tccatatgca tttcttgcag cgagcaagga tgtggtggat
    24481 gaggcctggc ccggcgatgt gattggttta tatgatacag gcaatttcaa aatcggcgat
    24541 acacttaccg aaggtgaaga cgtgatgttc cgtggcatcc caagtttctc tcccgaaatt
    24601 tttaaggagc tggtgaatat ggatccgatg aaaacaaaac agttggaaaa agg
//
LOCUS       DAOQOU010000033        24439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_971_length_24439_cov_5.315986, whole genome shotgun sequence.
ACCESSION   DAOQOU010000033 DAOQOU010000000
VERSION     DAOQOU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24439
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_24439_cov_5.315986"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1056
                     /locus_tag="PLM90_04725"
     CDS             238..1056
                     /locus_tag="PLM90_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ88704.1"
                     /translation="MEKIQITYAHPNSAGRLQHNLGIDAYRIVLLKGARGKLLVDCEY
                     YPLNPFTIAFVYPGQQFELPPQKPAEAWVISFEESFFYTNTEHKELLFRSPFFGYVLS
                     SPLFRINAKQYEMFASITSQMLHEQKLHAERSDQVMLSFLNIFLLYIKRMSWGKNTKV
                     HQNDYTTLQIVSRFKHLIRTHCHQQHSVKWYADQLQQTSNYLNLMVKKVTGGTAGDLI
                     ADQLTMEAKRLLMHTKLSPKEVAFTLGFYDHSYFSKFFKKQTGYNPLEWFNRNK"
     gene            complement(1081..2265)
                     /locus_tag="PLM90_04730"
     CDS             complement(1081..2265)
                     /locus_tag="PLM90_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88705.1"
                     /translation="MRNLEHYALLSGAFLAIGSNADAQVVYTDIDPDLYTDGSFQSLD
                     IDVDGIADFGFYMYFYTSWFDVQYSMVYMQALHSGAAIENAAYAWWIPSTGAIALDAG
                     AAIGSAGNFDMPPGEDLALLAALKSYNPGFSCSSSSFSPHDQLFFGFPGVGDKYVGIR
                     LGEPGNYHYGWIRLSVPYHIVCTVPMGQSFELIAHDYALNLTANASILAHEVAVDYCH
                     PPTPFTPLVGATAAKISWESVAEAEAYLVQYRQTGVATWTEKIISSTKTSKLIKSLNC
                     NTAYEWQVSSICDSGAVQSPPCALQTFTTGVCRIGVDEDVLTGITLFPNPVIDALHIS
                     AEEGIHSVIILNINGKLMKEQIFGSPEDISIDVADFPAGVYLIQLQTESGTSSDKFVK
                     AE"
     gene            complement(2375..5125)
                     /gene="ppdK"
                     /locus_tag="PLM90_04735"
     CDS             complement(2375..5125)
                     /gene="ppdK"
                     /locus_tag="PLM90_04735"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HMZ88706.1"
                     /translation="MSKQYVFGFDSGDGKNKKLLGGKGANLCEMTQIGLNVPPGFVIS
                     TEACLAYLDTSEHVLPEGVIEQVRSQMAAIEKASGKRFGDPENPLLVSVRSGSAMSMP
                     GMMDTILNLGLNTDTLKGLITQTGNERFGYDAYRRFIQLFGKVALGVPDEKFDVVFED
                     IKHNAGVKADIGLNTNNLKEASERFLKVVAEHTGRPFPADVYTQLEIAIKAVFNSWMG
                     KRAVDYRREFKITPEMANGTAVNVVTMVFGNMGNDSATGVGFTRDPGTGENVMFGEYL
                     TNAQGEDVVAGIRTPKAVQMLAKEMPEQYKQLEELRNKLESHYREVQDFEYTIEKGVL
                     YCLQTRNGKMNTTAMVRTSVEMAREGIISRSEALMRIKPAMLEQLLHPRLDSLQKHEP
                     LAQGLPASPGAASGHVVFDADRAEMLGRGGEKVILVREETKPEDIHGFFASQGILTSR
                     GGKTSHAAVVARGMGKPCVAGAEGIKVDVKMRQATIGEKVLHEGDYITIDGGTGFVYK
                     GEIPTVEPTFSDELKILLGWADEAAKLKVFANADTPEAAKKALHYGAMGIGLCRTERM
                     FNAPERLPIVVEMILANTTPEREKALDRLREIQKQDFKDILMTMAPRPVTIRLLDPPI
                     HEFLPTEDVLRDELRHLRHLRETASGVSNLFGSLRLLNVDPAMANTATQPFTDKGLEL
                     IDQAIAKKEQMLRKVRELYEVNPMLGHRGVRLGLTYPEIYTMQIGAILEAAAECQKVG
                     IDVRPEIMVPQVCAAKELAYVNKYVRQLKESIENQYNIQLHYKFGTMIEVVRACMEAD
                     SLAGEAEFFSFGTNDLTQATFSFSREDAENKFLPWYINSGVLKDNPFEILDEHGVGKL
                     MRFAVQEGRNTKPDLKIGICGEHGGQPEAIAFCHGLGLNYVSCSGPRVPVARLAAAQA
                     AM"
     gene            5424..6371
                     /locus_tag="PLM90_04740"
     CDS             5424..6371
                     /locus_tag="PLM90_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88707.1"
                     /translation="MKRTTLVALVCLLTGVMRAQTPSDAVMMNPGEICIDLHYGHSAW
                     DEYWEGDSLRDNGNIGTLNTSIYGTGFMLGLINRVNLLASLPYVVTNPTGGVISGDKG
                     IQDASFYLKGLLVEQKLGKGTFKTLASIGISLPAGDYVPDAPFAIGLGCPDGIFRGIL
                     HYDADMGLYGRVDAGYHLRGSAYLDRTYYYTTQAYYSDQVDMPNATDYNVTVGYITKN
                     KHFKAEAVYGGLTTFDGFDIRRQDGGFPSNDMEMSRVGVNLEYYDLFVKGLAIHAGGG
                     MVLTGSNVGKSTMFGGAISYQFPLWDGRDNKKSETPEAK"
     gene            6374..7924
                     /locus_tag="PLM90_04745"
     CDS             6374..7924
                     /locus_tag="PLM90_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ88708.1"
                     /translation="MKKLNYLVISGLAVSAFMASCTEDLPQYVGFEPYTYASLDEAGG
                     TWQPILLTSGADTTLTAPVDVTDPSYISELEEVKGYNGSLSADEQEAVDYWGNNTVIR
                     WQEIAQELIAKYNLAPSPNADGSYSSPSSLNPGVYPYFPFAHPPYATRVYAYLGAAQY
                     DALITAWNMKYTYNRPAPYDVDNSISPEYPDNTLPSWPSEDATVAEVSSELLKFLFPL
                     EVDYLNEMAAECRNSRKWAGMNVESDLVAGDAIGGFVFRKYKARAANDSMKFAQVDAA
                     TYNVMEAAAEATWGSMWPHWENLEVPQRPVGITPKYSSVTTWWIPSLEAVRPGPPPAM
                     NSEEYKAAEAELLDLTKNCTREQERIAYNWGDGFGTNTPAGHWGKIAADYMVSYRMNP
                     LRTARTYAYMNTAMMDAGISCWDTKYFYFYPRPPQANPEIKTLLGLPNFPSYTSGHAT
                     FSAAAGAVLAHIFPAEASEINGYVDEATESRIYGRIHFRFDCESGKTAGTSIGGYAVD
                     RMMTDGAE"
     gene            complement(8008..9795)
                     /gene="argS"
                     /locus_tag="PLM90_04750"
     CDS             complement(8008..9795)
                     /gene="argS"
                     /locus_tag="PLM90_04750"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMZ88709.1"
                     /translation="MTNLLEQLQKEVASVAEQLYATSFDAASIKIDKTPPDFDGEFTV
                     VVFTFVKASRKSPEATANELGAALMAGFSLIKSFNVVKGFLNISFTDDCWLQILSEIH
                     SAENFGQLPSTGKQVVVEYCGPNTNKPLHLGHIRNILLGYSTANILSAAGHSVHKVNI
                     LNDRGIAICKSMVAYKRNGNGSTPASIGMKGDHFVGKYYVEYSKIIEAEISALMNNAD
                     ISKDAAEKQAPIYLEAQEMLLKWEAGDSEVMALWNMMNSWVYSGFDITYKLIGIDFEK
                     EYKESEFYLNGKKMVLEGLNSGAFFKKEDGSVWVDLTSDGLDQKVLLRADGTSVYITQ
                     DMGVAEERYRDFTFDESIYVVANEQDYHFKVLKLVLQKLGRKYADGIHHMSYGMVDLP
                     EGKMKSREGTVVDADDLIEGMVSEAMDFLASSEKNAELNETEKQALAHDIGMSALKYF
                     ILKVDPKKRILFNPKESIDLHGNTGPFIQYAYARIRSILRKIDEVKVNIQVSIGTGLM
                     PAEKELIIILSQYPGIVKLAAETYSPAEIGNYLYLLAKKFNSFYADHSILKAESEEKM
                     HLRAVIALHVSLVIKHGLDLLGIHSPEKM"
     gene            complement(9792..10745)
                     /gene="rocF"
                     /locus_tag="PLM90_04755"
     CDS             complement(9792..10745)
                     /gene="rocF"
                     /locus_tag="PLM90_04755"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HMZ88710.1"
                     /translation="MRRVKVISVNSELGAGTRGAGLGFDAIRVAAWTKGSRYFKEHPP
                     LIIQSNNDEVLDDIETAYSVKIQFIVEMYKKISEAVVESMKKKEFPIIISGDHSNAGG
                     TIAGLKLAYPKETLGVIWIDAHADLHSPYTTPSGNLHGMPLATATALDNKVCQINQPE
                     KKTIEAWEKLKKLGGISPKITFKDLVYIAARDMEKAEKFILKKNNVRIYSVQKMRETG
                     IQSVIEDALKRLAHCDRIYVSFDVDSMDANVSRGTGTPVKSGLYESEAFSLMVALAKQ
                     EKVCCMEITEVNPTLDNKKNLMAEIALEILEQFTAEVEKRL"
     gene            10820..12229
                     /locus_tag="PLM90_04760"
     CDS             10820..12229
                     /locus_tag="PLM90_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HMZ88711.1"
                     /translation="MKNTYLDLIQQTFYFPQDGFEVKNNYLYFNGIPLKELIDKYETP
                     MRITYLPKISDNIQRAKRYFNDAIRKLNYPGTYNYCYCTKSSHFAHVMEEALKNDIHL
                     ETSSAFDIDIIEKLYLNKMVTKDTLIVCNGYKTAIYTQRISKLANLGFNITPVLDNKE
                     ELDAYQHTIIDSCDIGIRIASEEQPEFQLYTSRLGIRYSEIRDFYLNKIKTNPKFRLR
                     MLHFFINSGIKDNIYYWNELNKSLKVYIDLKRVCPELDGLNIGGGWPYRNSLGFEYDY
                     PYMAEEILNRIKQTCEEEKVECPNIFTEFGSFTVAESGAFIFQVLGEKRQNDSERWYM
                     IDNSLMTTMPDSWGINSKFILLPVNKWGNEVQRSNIGGLTCDQMDYYNSEAHTNELYL
                     PINTMGEPLYIGFFHTGAYQESISGYGGTKHCLIPSPKHILISRGEEGELKYEVFADE
                     QQVESMLTVLGYDKLDERD"
     gene            complement(12226..13566)
                     /locus_tag="PLM90_04765"
     CDS             complement(12226..13566)
                     /locus_tag="PLM90_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88712.1"
                     /translation="MISVQNRRQPLSGNNKYIIVLLATVFLFASCGIFNGAQHDSTVS
                     HPTNTDQTTVHADTVIRKKVDSVIIPTKVIDNLASEKKPAYDISFVLPFSIDAAELEK
                     LSSEENITGFQPLASIEFYEGALMALDTLDSLGIKLNVHVFNNYKDSSLTAALFSKPE
                     IMKSDAVFGPIFNESLKAASPVAQQNKFYLVSPLSPNNSFIDTNKYFMMANPTVEAQL
                     EAMLKSIETQHPFANIINIYRSDKPNEKHLADDFAAAFNIANTGKGMRLSNATSYAEA
                     TALMKSVENFVFISSYDELYVNGLIRDLSKFSRETPVEMLGLQHILSFESISIDYYEN
                     LHFTYPNAYYIDRNSPKVINFETAFIERYSTRPSDFACRGYDFTLYIAMMLNSYGVDI
                     SASADKLNPATPFMLDPVRFTGIKNNAGITRYMEKSNITILRYDQYIFKPLSTN"
     gene            complement(13523..15064)
                     /gene="guaA"
                     /locus_tag="PLM90_04770"
     CDS             complement(13523..15064)
                     /gene="guaA"
                     /locus_tag="PLM90_04770"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMZ88713.1"
                     /translation="MTEKILILDFGSQYTQLIARRIRELNVYSEIVPCTKIPEITPDI
                     KGIILSGSPFSVSSPEKPAFDIEAILDKRPILGVCYGAQFVAKHFGGVVQPSSKREYG
                     RAFLHIQDRNCELFYGIDDESQVWMSHSDTVMQVPAGFHVTAKTASIDVAAFASDPGR
                     FNNPMYCIQFHPEVTHSEQGKKMLQNFVVGTCGCHQTWTPESFVEETVAYLQTTIGSD
                     QVVLGLSGGVDSSVAAILLNKAIGKNLISIFVNNGLLRKNEFNSVLHSYQDMGLNVIG
                     VDAEDEFLSALEGESDPEKKRKIIGKVFIDVFERESKKLTNVKWLAQGTIYPDVIESV
                     SVHGPSATIKSHHNVGGLPEKLHLKVIEPLRFLFKDEVRKVGDTLQLKHEILHRHPFP
                     GPGLAIRIIGDITREKVRIVQEADAIFINHLKSSGWYDQVWQAGAILLPVQTVGVMGD
                     ERTYENVVALRCVNSTDGMTADWSHLPHTLLAQISSDIINHVKGINRVVYDISSKPPA
                     TIEWE"
     gene            15063..15311
                     /locus_tag="PLM90_04775"
     CDS             15063..15311
                     /locus_tag="PLM90_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88714.1"
                     /translation="MRKDSELQKRALLVIVYFEGYAGHRKNLGNGELFGEKFHTPVIQ
                     FPNLLLKLWNAKFAIWGDSTTKSGRIGPRFSDLHAGML"
     gene            15762..15845
                     /locus_tag="PLM90_04780"
     tRNA            15762..15845
                     /locus_tag="PLM90_04780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15796..15798,aa:Leu,seq:gag)
     assembly_gap    15992..16091
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16263..16484)
                     /locus_tag="PLM90_04785"
     CDS             complement(16263..16484)
                     /locus_tag="PLM90_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HMZ88715.1"
                     /translation="MLYHFKHLIETGVFGACSWLGEKLGIASSRVRLWFVYVSFLALG
                     SPIILYMIIAFWVNLKNYIRDKVSPVRDL"
     gene            complement(16510..17775)
                     /locus_tag="PLM90_04790"
     CDS             complement(16510..17775)
                     /locus_tag="PLM90_04790"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HMZ88716.1"
                     /translation="MQEAFIQFIWRMQLFTASTLTTTDGEQISVINRGTLNTDSGPDF
                     LHARIRIGDTLWVGNVEIHLRSSGWLSHKHHVDRAYDNVILHVVYEHDMPQSDIPTLE
                     LKNIIDNSLIDRYELMMQTASWIPCAKTIAHVPPVIIRQHLNRMLAERLEEKALRFEQ
                     RLMLNSNDWEETCYQLIARSFGTNVNADPFERLARSLPLKTILKHHDQPLQIEALLFG
                     QAGLLEGNFKSVYPHRLRAEYIFLKKKYGLESIRALEWKFLRMRPANFPTIRLSQLAA
                     FLAGREKVFSHILELRSVKEAMALFAVEASPFWQEHYHFKKATKKAPGGIGTDLVHLQ
                     VINTFAPLLFLYGKQTGNSDFIVRATDLLEQVPAENNSIVRQWKELGIHAKHAGESQA
                     LLHLKNEHCAHKHCLDCAVGYRILNQTNL"
     gene            complement(17962..18777)
                     /gene="pyrF"
                     /locus_tag="PLM90_04795"
     CDS             complement(17962..18777)
                     /gene="pyrF"
                     /locus_tag="PLM90_04795"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMZ88717.1"
                     /translation="MDRTQLIHQIKTKRSFLCVGLDTDLEKIPVHLLQESDPIFAFNK
                     AIIDATRAHCVAYKPNVAFYEAYGSKGWESLEKTIQYIGKEHFIIADAKRGDIGNTSA
                     RYAHAFFGAMSCDAITVAPYMGEDSVTPFFSYPDKWVILLALTSNKGSQDFQLIADGD
                     GALYEHVIRKAATWGNTGNLMYVVGATHPEYFSEIRKLIPDHFLLVPGVGAQGGDLHA
                     VCAHGMNTDCGLLINSTRDIIYASSGHDFAQAAAQRAMEISMQMNLLLQAKGI"
     gene            complement(18871..19113)
                     /locus_tag="PLM90_04800"
     CDS             complement(18871..19113)
                     /locus_tag="PLM90_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMZ88718.1"
                     /translation="MNGNEALIQQVEASLDSIRPYLNQDGGDIKVVDITDDMVVKVKL
                     LGACESCPMSFMTMKAGVEATLKKTVPEIKAIETVD"
     gene            complement(19147..20250)
                     /locus_tag="PLM90_04805"
     CDS             complement(19147..20250)
                     /locus_tag="PLM90_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HMZ88719.1"
                     /translation="MAISEADILKALSNVDDPDLHKDIVSLGMVKDITIDGTHIRFTV
                     ELTTPACPMKEEIRRACVNAVKLLVSKEAEVDVNMSARVSSIRGNQEQLLPGVKNIIA
                     VCSGKGGVGKSTVSVNLALGLAKTGAKVGIMDADIYGPSIPVMMGLKDAQPRVEEVNG
                     KPMMIPLEAHGIKALSIGFLVDETQAVVWRGPMVSSALRQFVTDCIWGELDYLIIDLP
                     PGTGDIHLTLVQTVPVTGAVIVTTPQEVALADARKAVGMFRLPNINVPVIGIVENMAW
                     FTPEELPENKYFIFGTGGGTQLAKEYDVPLLAHIPLVQGIREGGDSGKPAILRNDIPV
                     LTEAFDTLINNTARYISIRNEEKTATKIVEITR"
     gene            20392..22749
                     /gene="topA"
                     /locus_tag="PLM90_04810"
     CDS             20392..22749
                     /gene="topA"
                     /locus_tag="PLM90_04810"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HMZ88720.1"
                     /translation="MGKNLMIVESPAKAKTIEKFLGKDFVVKSCYGHIRDLEKADFGI
                     DFKNNYEPKYIISADKEAVVKELKKLAKSAEEIWLATDEDREGEAISWHLCEVLGLNA
                     KEAKRIVFHEITKKAIQNAVANPRNINIDLVDAQQARRILDRIVGFELSPLLWRKINM
                     KTSLSAGRVQSVAVRLIVEREQEIGKFNAMASFKVTALFHVKDDSGKTYQLKAELPAK
                     LDEEAQVKSFLQDCIGAEFQVGNITVKPAKRSASAPFTTSTLQQEASNKLGFSVSRTM
                     IIAQKLYEEGHITYMRTDSVNLSQQAIDAAQSEIIKNYGERYSNPRQYKTKSSGAQEA
                     HEAIRPTYMENQDVPGDFDEKRLYKLIWKRTMASQMADAELERTIVDIDISTRKEKLA
                     AKGEVIKFDGFLKVYTEALEDERDEDATILPPLKVGAPLTMIEMEGAQKFSKPPARYT
                     EASLVKKLEELGIGRPSTYASTISTVQKREYVVKKDKEGIAREAVQFKISEKQVVRSV
                     VTEMYGAERMKLFPTDLGILVTEFLIENFPQILDYSFTADVEAQFDDIAIGKREWHKM
                     IDAFYKPFHEHLELTTKTAQRVTGERILGAHPETGEQISVRMGKFGPMVVMGELLDPK
                     EAKAKGDGKKPKFAGLLKHQNMAEITLEDALKLFELPKTIGTYREDDVVVGVGRFGPY
                     VRNMKKFYSIPKGTEAAAITLEEAIQLINDKDLKDQNKLIHDFPEHEIQVLNGLYGPY
                     IKKGKDNYKIPKDRVAKDLTLEQILEIVETTEPSGKGKRKFAKKK"
     gene            22755..23630
                     /locus_tag="PLM90_04815"
     CDS             22755..23630
                     /locus_tag="PLM90_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMZ88721.1"
                     /translation="MEHREAEIQALIALLDDSDPEVFDHVAARLLSYGPMVIDKLEDA
                     YTTIPNPVMQERIEDIIHQIQFAGVLADMRNWAAHGHNDLMRGLLIITRHQYPDLDED
                     AILANVARIQKDIWIGINTYLSPLEQMNVINQSLFSQYQFAGLQNNDDELRYAYINNM
                     LDAFKGNHFSVGLLYLILCQQLDMPVYGVLLSTHFILARMKEDIVNFEGDNKDDILFY
                     INPYNKGLAFSEREIKGYLSKLEIPSDEKYFKPASNKAVLAEYIRHLISLIKKPQDQY
                     KIEDLEALLACLESE"
     gene            23652..>24439
                     /locus_tag="PLM90_04820"
     CDS             23652..>24439
                     /locus_tag="PLM90_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ88722.1"
                     /translation="MAEKVTISGFSFVRNADRLFYPVKESILSILPLVDEFVIAVGDN
                     APGDGTIALIESIQSPKIKILHTVWDTETYPKATIYAQQTNVAMKACTGDWLFYIQGD
                     EVVHEKDLDAIRKQCEKYADDPEVEGLLFDYYHFWADYAHINRSHSAYAQEIRMVKNR
                     KDIFSYGDAQGFRRIPGFDMRNFRQKENVFKLKVAPAQAHIYHYGWVRPPDFMMRKKQ
                     FANTLHHGQAETDANYKAAMFDYGPVGRKFPFKDTHPAVMAERV"
BASE COUNT         6893 a         5459 c         5059 g         6928 t
ORIGIN      
        1 ctcactccca atctcctcaa agcccgagaa accgcttata tccgattact cgcgatctcc
       61 tccaaaagag cctccgcata tcaaaaatga gccctcatac ccaatttttt gctatcgtga
      121 aaaaatacta ctaaatcgtg aaaaaatacc ggcgataaac gatatatttg taaatgaaag
      181 catgatacaa atgccctcct agttttttaa gcattcttgt ttttacaagt atcatgtatg
      241 gagaaaattc aaatcactta cgctcatcct aattccgccg gccgtttaca gcataacctc
      301 ggcatagatg cttatcgcat tgtcctactc aaaggcgcac gagggaaatt actcgtcgac
      361 tgtgaatatt atccacttaa cccatttacc atcgcttttg tgtacccagg acaacaattt
      421 gaattgccgc cgcaaaagcc ggctgaagca tgggtaatct cctttgaaga gtctttcttc
      481 tatactaata ctgaacataa agaactactc tttcgctctc cattttttgg ttacgtcctt
      541 tcttcacctc tgtttagaat caatgccaaa caatatgaaa tgttcgcctc tatcacctca
      601 caaatgctgc atgagcaaaa attgcatgcc gaaagaagtg atcaggtgat gctgagtttc
      661 ctcaatatat ttttgctgta tatcaaacgc atgagctggg gaaaaaatac taaggtccat
      721 caaaatgatt ataccaccct tcaaatagtt tcacgcttta aacacttaat ccgcactcat
      781 tgccatcaac aacatagcgt gaaatggtac gccgatcaac tccaacaaac ctcaaactat
      841 ctaaacctca tggtgaaaaa agttacaggt ggaacggcag gagatcttat tgctgatcaa
      901 ttaaccatgg aagcgaagcg cctactcatg cacacgaagc tctctccaaa agaagttgct
      961 tttacactcg gcttttacga ccattcctat ttttctaaat tcttcaaaaa acaaacagga
     1021 tacaatccgc tcgagtggtt caatcgaaat aaatgacacc ggtaatattg ccattcgcct
     1081 ttattcagct tttacaaact tgtcagaact tgttcctgat tcggtttgca attgtatcaa
     1141 atacacgcct gcaggaaaat ctgcaacatc tatcgaaata tcttctggac taccgaaaat
     1201 ctgctctttc atcagctttc cgtttatatt taatataatc acggaatgta tcccttcctc
     1261 ggcggaaata tgtaaagcgt caatcaccgg attcggaaac aaagtaattc cggttaatac
     1321 atcttcgtcg acgcctatcc tgcatacccc tgttgtaaat gtttgcagtg cacatggtgg
     1381 cgattgaact gctccgctat cgcaaattga acttacctgc cattcgtaag cggtattgca
     1441 atttagcgat tttatgagct tggatgtttt ggttgatgaa atgattttct cagtccaagt
     1501 agcaactcct gtctgccgat attgaactaa gtaagcctcc gcttccgcaa ctgattccca
     1561 ggaaatcttg gcagctgttg cgcctacaag cggcgtaaat ggagtagggg gatgacaata
     1621 atccaccgca acttcgtggg ctagtatcga tgcatttgct gttaggttca aagcataatc
     1681 atgggcaatt aattcaaacg attgtcccat aggaactgtg cacacaatat ggtatggcac
     1741 cgataaacga atccaaccat aatggtaatt accgggctcg cctaagcgga tacctacata
     1801 tttatcgcct actccgggaa agccgaaaaa caactgatcg tgcggtgaaa atgacgatga
     1861 gctgcacgaa aacccgggat tataagactt taaagcagcc agtaatgcca aatcttcacc
     1921 gggaggcata tcaaaattgc ctgcggaacc aatcgctgcc cctgcatcca aagcaatggc
     1981 tcccgttgat ggtatccacc aggcataagc cgcattttca atcgcagcac cactatgtaa
     2041 agcctgcatg tataccatcg agtattgcac gtcaaaccaa ctcgtataga aatacatgta
     2101 aaacccaaaa tcggcaatgc catctacatc aatatccagc gattggaaac taccgtcggt
     2161 gtaaagatcc ggatcaatat cggtgtatac tacctgtgca tcggcattag agccaattgc
     2221 aagaaaagca cccgataaca gtgcatagtg ttcgaggttg cgcataatgt gatagcttaa
     2281 tcacatgaag ctacactata caactcccaa acggaatgat ttctgtcaac atcctaaaac
     2341 cgcaccccgc acctttagtt gttctcccta aacatcacat cgccgcctgc gccgctgcca
     2401 atcttgcaac cggtaccctt ggacctgaac agctcacata gttcaatccc aatccgtgac
     2461 agaaggcaat cgcctcaggt tggcctccat gctctccgca aataccgatt ttcaaatccg
     2521 gtttggtgtt tctaccttcc tgaaccgcaa atcgcatcag cttgcctaca ccatgttcat
     2581 ccaggatctc aaatgggttg tctttcaaca ctcctgaatt gatataccaa ggcaggaatt
     2641 tgttctcggc atcctcgcga ctgaagctga aagttgcctg tgtgaggtcg ttggtaccaa
     2701 agctgaaaaa ctcggcttca ccggccaaac tatccgcttc catacacgct ctcaccacct
     2761 caatcatggt accaaatttg taatgtaatt gtatattata ctgattttca atactttctt
     2821 taagttgtct aacatatttg tttacatacg ctagctcctt cgcagcacat acttgaggta
     2881 ccataatctc aggtcttacg tcgatcccaa ccttttggca ttcggcggcg gcctccagaa
     2941 tagctccgat ctgcatggtg tagatctcag ggtaagtgag tccgagacgc acacctctgt
     3001 gaccaagcat tgggttcact tcatacagtt cgcgcacctt gcgaagcatc tgctcttttt
     3061 tggcaatggc ctggtcgata agttcgagtc ctttgtcggt gaatggctgt gtagcagtgt
     3121 ttgccatggc cgggtcaaca ttcagcagtc gcaggcttcc gaagaggttg ctgactccgg
     3181 aagcggtctc ccggaggtga cggaggtgtc taagctcatc gcgcaacaca tcttctgttg
     3241 ggaggaattc atgaataggt ggatcgagta gacggatggt gactggacga ggagccatgg
     3301 tcatgagtat atccttgaag tcctgtttct gtatctccct caatctatcc agcgcttttt
     3361 cacgttccgg tgttgtgttt gcgagtatca tctccactac gattggcagg cgctcaggcg
     3421 cgttgaacat gcgttctgtg cgacaaagtc caatacccat ggcaccgtaa tgcagtgctt
     3481 tcttggcggc ctcaggggta tcggcattag cgaatacctt taacttggca gcttcatccg
     3541 cccatccgag caagatcttc aattcatcgc tgaaggttgg ctcaacagtt ggaatctcgc
     3601 ctttatacac aaatccggtt cctccatcga tagtaatata gtcgccttcg tggagtactt
     3661 tttcgcctat tgtagcctga cgcatcttca cgtcaacttt tattccctcg gccccggcaa
     3721 cgcatggttt tcccatacct cgcgcaacta ctgcggcgtg tgaggtcttt cctccgcgag
     3781 aggtaaggat accctgactg gcaaagaatc catgtatatc ctccggtttg gtctcctcgc
     3841 gcaccaggat caccttctcc ccaccgcgac cgagcatttc agccctgtcg gcatcgaaaa
     3901 caacatgtcc ggaggctgct cccggagagg ctggtaaacc ctgtgcaagc ggctcatgct
     3961 tttgcaggct atccaggcgt gggtgtaaca attgctctaa catggcaggt tttatgcgca
     4021 tcaatgcctc tgagcggcta ataatgcctt ctctggccat ttcaacgctg gttcttacca
     4081 tggcagttgt gttcatcttg ccatttcgcg tttgcagaca gtacaggacg cctttttcaa
     4141 tcgtatattc aaaatcttgc acctcgcggt aatgactctc taacttgttg cgtagctcct
     4201 ccagttgctt atactgctca ggcatctctt tagccagcat ctgcaccgct tttggcgtgc
     4261 gaattcctgc taccacatct tccccctgcg cattggtcaa atattcacca aacatcacat
     4321 tttctcctgt ccccggatcg cgggtgaaac caacgccggt ggcactgtca tttcccatgt
     4381 ttccaaaaac catcgttacc acgttcaccg cagttccatt tgccatctca ggtgtgatct
     4441 tgaattcgcg acggtaatca accgctcttt ttcccatcca actgttgaat acagccttga
     4501 tagcaatctc tagttgagta tatacatcag cagggaatgg cctgcctgta tgctcggcaa
     4561 ctaccttcaa aaatcgttca gatgcttctt ttaaattatt ggtattcaag cctatatcag
     4621 ctttaactcc tgcattgtgt ttgatatctt cgaatacgac atcgaacttt tcatcgggaa
     4681 cgccaagtgc tacttttcca aacaattgga taaaacgacg ataagcatcg tatccgaatc
     4741 gctcgttgcc ggtttgggtg atgaggcctt ttaaggtatc agtgttaagt cccaggttga
     4801 gaatagtatc catcatcccg ggcatcgaca tggctgaacc ggaccgcacg gatactaata
     4861 agggattttc aggatcgcca aaacgctttc cacttgcttt ctcgattgcc gccatttgcg
     4921 accttacctg ttctattact ccttcaggca atacatgctc tgaggtgtca aggtaggcta
     4981 aacaggcttc cgtgctgatg acgaatccgg gcggcacatt taaacctatc tgggtcatct
     5041 cacacaggtt tgcccctttt cctcctaaca attttttgtt ttttccgtcg ccggaatcaa
     5101 atccaaatac atattgcttg ctcatatcat ggtattttga agttacttag aatgagggct
     5161 aagttagatt gatgccaacc tgccggaaat gacttgggtt tcctatcata gaagctaaag
     5221 tcatcattta aagtacacaa gcgtacactc gaatccgcta tttagatatt gccgacgaaa
     5281 actgaagaac tagtttatta aaccttaaaa gaagttcaaa ctctttaaaa ctaatgtaaa
     5341 ctatttagct caaagcctta tgccatctat ttttgaccgc attaaaagac aactactaaa
     5401 acttattgat ttaaatgaat cttatgaaaa gaactacact ggtggcgtta gtatgcctgc
     5461 ttacgggtgt aatgcgcgct cagactcctt ctgatgccgt tatgatgaat cccggagaga
     5521 tctgcatcga cctgcactat ggtcattccg cttgggatga gtattgggaa ggcgattctc
     5581 tgcgcgacaa tggcaatatc ggtacactca acacaagtat ttatggcacg ggatttatgc
     5641 tcggactcat taaccgagtg aacctgctcg catctttacc ctacgtcgtt accaacccca
     5701 caggcggggt gatttccggc gataaaggaa tccaagacgc atctttctac ctaaaaggac
     5761 ttctggtaga acaaaaacta ggcaaaggta cctttaaaac ccttgcaagc attgggatct
     5821 cattgcctgc aggtgattat gtgccggatg ccccatttgc tatcggcctc ggctgtccgg
     5881 atggtatttt ccgcggtatt cttcattacg atgccgacat gggattatat ggccgcgtag
     5941 atgcaggcta tcacctccgt ggctcagcct acctcgatcg cacctattat tacaccacgc
     6001 aagcctatta ctcagatcaa gtagacatgc caaatgctac cgattacaat gtaacagtag
     6061 gctacatcac caaaaacaag cacttcaaag cagaggctgt ttatggcgga ttgacaactt
     6121 tcgatggctt cgatattcgt cgtcaggatg gcggattccc ttctaatgat atggaaatga
     6181 gccgagtggg tgtgaatctt gaatactatg acctgtttgt taaaggtctg gcaatacatg
     6241 ccggcggcgg aatggtgctt acaggaagca atgtaggcaa gtctaccatg ttcggcggcg
     6301 ctatttctta ccagtttcct ttgtgggacg gtcgcgacaa taaaaaatct gaaacccctg
     6361 aagcaaaatg atcatgaaaa aattaaacta tctcgtaata tccggactcg ctgtttcagc
     6421 atttatggca tcgtgtaccg aagacctgcc gcaatacgtg ggttttgaac cttataccta
     6481 tgcgtcattg gacgaagccg gcggaacatg gcaacctatt ttattaacta gtggggctga
     6541 tacaacccta actgctcctg ttgatgtaac tgatccgtct tatatttctg aattggaaga
     6601 agtgaaaggc tacaatggct cactttcagc cgatgagcag gaagctgttg attattgggg
     6661 caataacacc gtaattcgtt ggcaggaaat tgcacaagag ctcatagcaa aatacaacct
     6721 tgcaccatca cctaatgctg atggatccta tagctcccca agttcattga atcccggcgt
     6781 ttatccttat ttcccgtttg cgcatccacc atatgccacg cgtgtgtacg catacctggg
     6841 cgccgcgcaa tatgatgctt tgatcactgc gtggaatatg aaatatactt acaatcgtcc
     6901 tgcaccttat gatgtagaca acagcatctc tcctgaatat cctgataaca ccctgccttc
     6961 atggccttca gaggatgcta ccgttgcaga agtgtcttcc gaattactca aattcctctt
     7021 ccctttggaa gttgattacc tgaatgaaat ggctgcagaa tgtcgcaatt cacgcaaatg
     7081 ggcaggtatg aacgttgaaa gcgacctggt tgccggcgat gccatcggag gatttgtttt
     7141 ccgtaaatac aaggcgcgtg ctgccaacga cagtatgaaa ttcgcacaag tagatgctgc
     7201 aacttacaac gtaatggaag ccgctgccga agctacctgg ggctctatgt ggccacattg
     7261 ggaaaacctt gaagtacctc aacgcccggt aggtattaca cctaaatata gctctgtaac
     7321 tacctggtgg attccttcgc ttgaagcagt gcgccccggt ccgccacctg ccatgaattc
     7381 tgaagaatat aaggctgctg aggctgaatt gttagacctc accaaaaatt gtactcgtga
     7441 gcaagaacgc atcgcctata attggggtga tggtttcggc accaacactc cggcaggcca
     7501 ttggggaaaa attgctgccg attacatggt aagctaccgc atgaatccat tgcgaaccgc
     7561 aagaacttat gcttatatga acactgccat gatggacgcc ggtatctcat gctgggatac
     7621 gaagtatttt tatttctacc ctcgtccgcc tcaggcaaat cctgaaatca aaacactgct
     7681 tggattacca aatttcccat cctatacttc cggccacgca acgttttcag cggccgcagg
     7741 tgcagtgtta gcccatattt tccctgctga agcatctgaa attaatggat atgttgatga
     7801 agctacagaa tcaagaattt atggtcgcat tcactttagg tttgattgcg aaagcggtaa
     7861 aactgccggt acttctatcg gtggatatgc cgttgatcgt atgatgactg acggagctga
     7921 ataaactttc cctgtttatc atatatgagg tcatccttcg gggtgacctc tttttttgtg
     7981 ggaaggattg caacgtgtag gaagaaatta catcttctca gggctgtgta tacccaacag
     8041 atcaagaccg tgtttgatca ctaatgaaac atgtaaggct ataaccgcac gcaaatgcat
     8101 tttctcctcc gattcagctt tcaaaatgct gtgatcggcg tagaaggaat tgaatttttt
     8161 cgccaatagg tataaataat tgcctatttc agctggtgaa taggtttctg ctgcaagctt
     8221 tacgatgccc ggatattgac tcaagataat tatcaattct ttttctgcag gcatcaaacc
     8281 ggtgccaatc gaaacctgaa tgttaacctt aacctcatca attttcctta aaatactcct
     8341 gattctagca tacgcatatt gaataaaagg tcctgtgttc ccatgaaggt caatagactc
     8401 cttcgggtta aataatatgc gctttttagg atctactttc aggatgaaat atttgagtgc
     8461 cgacatgcca atatcgtgcg ccaatgcctg cttttcggtt tcatttagct ctgcattctt
     8521 ttctgaggat gcaagaaaat ccatcgcctc actaaccata ccttctatca aatcatcggc
     8581 atctactacc gtgccttctc gacttttcat tttgccctca ggaagatcca ccatgccgta
     8641 gctcatgtga tgaatgccat cagcatattt ccttcccaat ttttgcaata ccagtttgag
     8701 taccttgaaa tgataatcct gctcgtttgc aactacataa atgctttcat caaaagtgaa
     8761 atcgcggtat cgctcctctg caacacccat atcttgcgtg atgtaaacgc tcgttccatc
     8821 agcccttaac aatacctttt ggtcaagccc atcggatgtg agatcaaccc agacactgcc
     8881 atcctctttt ttaaagaaag cgccactgtt caatccttcg agcaccattt ttttaccatt
     8941 gagataaaat tcgctctctt tatactcttt ctcgaaatca atgccgatca gtttataggt
     9001 gatgtcaaag ccgctgtaca cccagctgtt catcatattc cagagagcca ttacttcaga
     9061 atctcctgct tcccatttca aaagcatttc ctgtgcttcg aggtaaattg gagcctgttt
     9121 ttcggctgca tccttactga tatctgcatt attcatcaaa gcagaaattt ccgcttcaat
     9181 gatcttgcta tactcaacat aatatttgcc cacaaaatga tcacctttca tcccgataga
     9241 tgccggggtg ctgccatttc cattgcgctt gtaagctacc atgctcttgc aaattgcaat
     9301 tccacggtca ttcagaatat taaccttatg gacactgtgt cctgcggcgc ttaaaatatt
     9361 tgcagtagaa taccctaaca gtatattacg gatatgtcct aaatgcaagg gcttattagt
     9421 attcggacca caatattcaa ctacgacctg tttgccagtt gaaggaagct gaccaaaatt
     9481 ttctgcgcta tgaatctcgc ttaatatctg caaccaacaa tcatcagtga agctgatatt
     9541 aaggaaaccc ttaaccacat taaatgattt gattaggctg aaaccggcca tcagggcagc
     9601 tcccagctca tttgccgtag cctcggggct tttgcgactt gcctttacaa aggtgaaaac
     9661 tacaacagta aactcaccat caaaatcagg tggtgtctta tcaattttaa tcgatgccgc
     9721 atcgaatgat gttgcatata attgctctgc tacagaagct acttccttct gtaactgttc
     9781 caataaattg gtcataacct tttctccact tcagccgtaa attgttccag aatttccagc
     9841 gcaatttctg ccatcaaatt cttcttgtta tcaagtgtcg gattcacctc agtgatctcc
     9901 atgcaacata ctttttcctg tttggctagt gctaccatga gcgagaatgc ctcactttca
     9961 taaagaccgc tctttacagg tgttccggtt ccgcgtgaaa cgttggcatc catactgtcg
    10021 acatcaaagg atacataaat ccgatcgcag tgtgccaaac gcttgagtgc atcctcaatc
    10081 actgattgta tgcctgtttc acgcattttt tgtacggaat aaatgcggac attatttttc
    10141 ttgaggatga atttttcagc cttctccatg tcgcgcgcag caatgtaaac aaggtctttg
    10201 aaggtgattt ttggtgagat gcctcccagc tttttcaact tctcccaagc ttctatcgtt
    10261 ttcttctcag gctggttgat ctgacaaacc ttgttatcta atgctgtggc agtggccaat
    10321 ggcataccat gcaaatttcc tgatggggta gtgtaagggc tatgcagatc ggcatgagca
    10381 tctatccaga ttactcctaa ggtctccttc ggataggcca acttcaatcc ggcgatagtt
    10441 ccaccggcgt tgctgtgatc gccactgatg ataatgggga actccttctt cttcatcgac
    10501 tccacaacgg cctcagagat ctttttgtac atctccacaa tgaactgtat cttaacagaa
    10561 taggctgttt cgatatcgtc gagcacttca tcattattgc tctggatgat cagcggaggg
    10621 tgctctttga agtacctgct gcctttcgtc catgcagcta ccctgatggc gtcaaatcct
    10681 agtcccgccc cgcgggtacc tgcgcctaat tcgctgttta ccgagataac ttttactcgt
    10741 ctcataccat gcaaagttat tatttttgtg ctgctaaagt aatctatcgt tttttcacgt
    10801 aaaatcaacc actcagacat tgaagaacac ttaccttgac ctcatccagc aaaccttcta
    10861 cttcccgcag gatggattcg aagtaaaaaa caattatctc tactttaacg gcatcccgtt
    10921 gaaagagtta atagacaaat atgaaacgcc gatgcgcatt acatatttgc cgaaaatatc
    10981 tgataacatt cagcgcgcta aacgctattt taatgacgcg atccgcaagc tgaattatcc
    11041 cggaacatac aattactgct attgtaccaa gagctcgcat tttgcgcatg tgatggaaga
    11101 ggcactgaag aatgatattc acctcgaaac atcatctgct ttcgatatcg atatcatcga
    11161 aaaattatac ctgaataaaa tggttaccaa agacacactt atcgtgtgca atggatacaa
    11221 aacggccatt tatactcaac gtatcagcaa gctcgcaaac ctcggattta atatcactcc
    11281 tgttttagat aataaggaag aactagatgc ctatcaacat accattatcg atagttgtga
    11341 tattggtata cgcatcgctt ctgaagaaca accggaattc cagttatata cgtcgcgact
    11401 cggtattcga tatagtgaga ttcgcgattt ttatctgaat aaaataaaaa caaatccaaa
    11461 attccgtttg cggatgttgc acttctttat caattcagga attaaggata atatctatta
    11521 ctggaatgaa ttaaataaat cgctgaaggt ttatatcgac ctgaaacgcg tttgccctga
    11581 actcgatgga ctgaatatcg gcggtggttg gccatataga aattctttgg gatttgaata
    11641 cgactatcca tatatggcag aggagatcct caaccgtatc aaacaaacct gcgaagaaga
    11701 aaaagtagaa tgtccgaata ttttcactga attcggaagc tttacagtgg cagaaagcgg
    11761 cgcattcatt ttccaggtgc tcggtgagaa gcgtcagaac gatagcgaac gctggtatat
    11821 gatcgacaat tcactcatga ccaccatgcc ggattcctgg ggcatcaact cgaaattcat
    11881 tttgttgcct gtaaataaat ggggtaatga agtgcaacgt agtaatatcg gcggacttac
    11941 ctgcgatcag atggattatt ataattcaga agcgcatacc aacgaattat atctcccgat
    12001 aaatactatg ggtgaaccat tgtatatcgg attttttcat acaggtgcat atcaggaaag
    12061 tatctccgga tatggtggaa caaaacactg cctgattcct tcgccaaaac atattcttat
    12121 tagtcgaggt gaagaaggag agctgaaata tgaagttttt gccgatgagc aacaagttga
    12181 aagtatgcta actgtgcttg gctacgataa acttgatgag cgcgattaat tagtggatag
    12241 cggtttgaaa atatattggt cgtaacgcaa tatggtgata ttactttttt ccatataacg
    12301 cgttatacct gcattatttt tgattcctgt aaatcgaacg gggtcaagca taaatggcgt
    12361 agcgggattt aatttatcgg cagatgcact aatgtcaaca ccatagctat tcaacatcat
    12421 tgcaatgtat aaagtaaagt cataaccgcg acatgcaaaa tctgatggcc gcgtactgta
    12481 tctttcaata aatgccgttt cgaaattgat tactttgggc gaattcctat caatataata
    12541 tgcattagga taggtgaaat gtaggttttc ataataatca atagagattg attcaaaaga
    12601 aaggatatgt tgcaacccca acatttctac cggcgtttct cttgaaaatt ttgacaagtc
    12661 tcgtatcaat ccattaacat atagctcatc ataagatgat ataaatacaa agttttctac
    12721 cgatttcatt aaagcagtag cttcagcata gctagtggca tttgacaatc gcattccctt
    12781 acccgtgttg gcgatattaa aagcagcagc gaaatcatcg gccaggtgtt tttcatttgg
    12841 tttatctgag cgataaatat ttataatatt agcaaacggg tgttgtgttt caatcgattt
    12901 caacatagct tccagttgtg cctcaactgt cggattggcc atcatgaaat atttattggt
    12961 atctatgaaa ctattgttcg gagataaagg tgatacaaga tagaatttat tttgctgagc
    13021 taccggagat gccgccttca gactttcatt aaatatcggt ccgaatactg catctgattt
    13081 cattatttca ggtttgctga aaagtgctgc ggtaaggctg ctgtctttgt agttattaaa
    13141 tacgtgcaca ttcaacttga tgccaagact gtcaagtgtg tctaaagcca ttaatgctcc
    13201 ttcataaaat tctatggatg ccaaaggttg aaagcctgta atattttctt ctgaactaag
    13261 tttttcaagt tcagcagcat caatactaaa tggcaataca aaagatatat cgtatgccgg
    13321 ttttttctcc gatgcgaggt tgtcgattac cttggtcgga ataattacac tatccacttt
    13381 ttttctgatc accgtatctg cgtgcacagt tgtttgatca gtatttgttg ggtgtgaaac
    13441 cgtgctatca tgttgagcac cattgaaaat gccgcatgat gcaaacagaa atactgtagc
    13501 taataacaca atgatgtatt tattattccc actcaatggt tgccggcggt tttgaactga
    13561 tatcatacac aacacggttg atacccttaa catgattgat aatatcactc gaaatctgtg
    13621 ccaataatgt atggggaaga tgactccagt ctgcagtcat tccatctgtt gaattaacgc
    13681 atctcaaggc tacaacattt tcgtatgtgc gctcatctcc cataacacct acggtctgca
    13741 ccggcaataa aattgcacct gcctgccaaa cctgatcata ccatcctgat gattttaggt
    13801 ggttgatgaa aatggcatca gcttcctgca caatgcgcac tttttcacga gtgatgtcac
    13861 caataatacg aatggccaat cccgggccgg ggaatgggtg gcgatgtaat atttcgtgtt
    13921 ttaattgaag tgtatcacca accttgcgca cttcatcttt aaataagaaa cgcagtggtt
    13981 ctattacttt aaggtgtaac ttttcaggaa gtccgcctac attatgatgc gatttaattg
    14041 ttgcagaagg tccatgcact gaaactgatt ctatcacatc cggatatatg gttccctgcg
    14101 caagccattt tacgttggtt agttttttag attcacgctc aaacacatca ataaacactt
    14161 tacctatgat cttgcgtttc ttttccggat cactttcacc ttcaagagct gaaagaaatt
    14221 catcctctgc atcaacacct atcacattca aacccatatc ctggtagcta tgcaataccg
    14281 agttgaattc attcttgcgc agtagcccat tatttacgaa tatcgatatg agatttttcc
    14341 caatggcctt atttaataat attgcggcaa cactggaatc tacaccacca ctaagtccca
    14401 atactacctg gtcactgccg attgtagttt gcaaatacgc aactgtttct tcaacaaacg
    14461 attccggtgt ccatgtttga tgacatccac aagttccaac cacaaaattt tgcagcatct
    14521 tttttccctg ttcgctatgt gttacttcag ggtggaattg tatgcaatac atcggattgt
    14581 taaatcgtcc ggggtcggag gcaaaagcgg caacatctat tgaagctgtt tttgcggtta
    14641 catgaaatcc tgccggcact tgcataacgg tatcactgtg actcatccac acttggcttt
    14701 cgtcgtctat gccataaaat aactcgcaat tgcggtcctg gatgtgtaga aatgctcggc
    14761 catattcacg ttttgatgat ggctgcacca ctcctccaaa atgcttggca acaaactggg
    14821 caccatagca aacaccgagt atcggacgtt tatcaagtat tgcttctata tcgaatgccg
    14881 gcttttccgg agaagaaacc gaaaaagggc ttccggataa gatgatacct ttaatatcag
    14941 gggtaatttc aggaatcttg gtgcagggga cgatctcgct atacacattg agctcccgga
    15001 tccttctggc gatcagttga gtatactgtg agccgaagtc cagaatcaga attttttcag
    15061 tcatgcgcaa agatagcgaa ttacaaaaac gagcactatt ggtaattgtg tatttcgaag
    15121 gttatgccgg gcacagaaag aatttgggga atggggaatt atttggggaa aaattccaca
    15181 ctcctgtgat acaattcccc aatttgttgc tcaagctgtg gaatgcaaaa tttgcaattt
    15241 ggggtgattc aactaccaaa tcgggtagaa taggacctcg ttttagcgat ttacatgccg
    15301 gtatgcttta gcagtaatgc cttgaaacgc ctgaaaataa aggatttttg gggttttggg
    15361 aataggctga gatatctaca aaaaaaaagc aggataaacc gctgtggctt accctgctat
    15421 ccccatgaaa acacacacac ttactataat gcaatctgtg tgccaagtgc cattatctgt
    15481 ataaattgaa atgttgagtg aaggtgtaat gctgctgtac tgtggattta gcggcaggat
    15541 gggatatcta caaaaaaaaa gcaggataaa ccgctgtggc ttaccctgct atccccatga
    15601 aaacacacac aataatagta tatgcaaatg ccgtgccaat gataaatttt tatcgatatg
    15661 ccttttattc tgcaaaattt ataaaatctc agacgctgct tgccaacctt ctgatttcat
    15721 tttcgagaat tgaaaacatt cgctaacttt gcacccgcct tgcccaggtg gtggaattgg
    15781 tagacacact agcttgaggg gctagcgcca gcaatggtgt gcaagttcga atcttgtcct
    15841 gggcacgcag atggtcggta tttatgccga ccttttttat tttacgcaag gtgtgcaagt
    15901 tccctgcccg cctcctgcgt cgtcaggtca ggcggggaat cttgtcctgg gcacgcagat
    15961 ggtcggtatt tatgccgacc ttttttattt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nacgcaaggt gtgcaagttc cctgcccgcc tcctgcgtcg tcaggtcagg
    16141 cttggaatct tgtcccgggc acgcagatag tcgttagtaa tgccgacctg atacgcttta
    16201 ttgcgtcagt attttcaagg atgtcgcccc ctaaatttca atgctttatt gacaggtaca
    16261 tatcaaagat ccctcacagg acttaccttg tcgcggatat agttcttcag atttacccag
    16321 aaggcaatga tcatatataa tatgatgggt gaccccaaag caaggaatga aacgtaaaca
    16381 aaccatagac gcacgcggct tgaggctatt cccagtttct ctcccagcca tgaacaggcg
    16441 ccaaatacgc cggtttcgat cagatgtttg aaatggtaga gcatatattc ccttctgttt
    16501 acaaatgtac tacaaattcg tttgatttag aatacgataa ccaacagcac aatcaagaca
    16561 atgtttatgg gcacaatgtt catttttgag atgtaacagt gcctgactct ctccggcatg
    16621 tttagcatgt atacccaatt ccttccattg acgaacgata ctgttattct cggcaggcac
    16681 ttgttccagt aaatcagtgg ctcttacaat gaaatcagaa ttacctgttt gtttgccgta
    16741 gagaaataat aacggtgcaa atgtgttgat cacctgaaga tgcacaagat cagtgccaat
    16801 gccaccggga gctttttttg ttgccttttt gaagtgataa tgttcctgcc aaaatgggga
    16861 agcttctacc gcaaataacg ccatcgcttc ctttacactg cgtaattcca aaatatgaga
    16921 aaatactttt tcccgaccgg caagaaatgc tgcaagctga cttaatcgaa tagtaggaaa
    16981 atttgcaggg cgcatgcgaa gaaatttcca ttccaatgca cgtatcgact cgagtccgta
    17041 tttctttttc aggaaaatgt attcagctcg caagcgatgt gggtacaccg atttgaaatt
    17101 tccctccagc aatcccgcct gtccaaataa taatgcctca atttgcaacg gctgatcatg
    17161 atgctttaag atagttttta gcggaagact tcttgcaagc ctttcaaatg gatctgcatt
    17221 tacatttgtg ccaaaagaac gtgctatcaa ttgataacat gtttcttccc aatcattgct
    17281 gtttaacatt aatcgctgtt caaatcgcaa agctttttcc tccagcctct ctgccaacat
    17341 ccgatttaaa tgctgtctga taataacagg tggaacatgt gcaatagttt ttgcgcaagg
    17401 tatccatgaa gctgtctgca tcatcaattc atacctatca ataagtgaat tatcaatgat
    17461 attttttaat tccaaagtag gaatgtcact ctgtggcata tcatgctcgt acacaacatg
    17521 caaaatcaca ttgtcataag ctcggtcgac atgatgttta tgcgaaagcc atccggaact
    17581 gcgcaaatga atttctacat tcccaaccca caaggtatcg cctatgcgta tgcgcgcatg
    17641 taagaaatca ggaccactat ctgtattaag tgttcctcta tttatgactg aaatttgctc
    17701 gccgtcagtg gtggtaaggg tggaagcggt aaataactgc atcctccaga tgaactggat
    17761 aaacgcttct tgcataatgt tgaattttta agggtaaaca ataggataaa catgaaacga
    17821 aaatacgaaa aataggggaa aggagagagg agaaaggaga gaggaaaaag gagagaggaa
    17881 agaggagaga ggaaattgtt atattgaaaa agaattctgc gcctctgcgt gaaactaaaa
    17941 ttggaatctt gataaaataa attaaatccc tttagcctgc aataacaaat tcatttgcat
    18001 gctaatttcc atggcacgtt gtgctgccgc ttgtgcgaaa tcgtgtcctg aagatgcgta
    18061 aataatatcg cgagtggaat taatcagcaa tccgcaatct gtattcatgc catgtgcgca
    18121 aacagcatgc aggtcgccac cttgtgcacc tacgccgggc acgagtaaaa aatgatcagg
    18181 tattaatttc ctgatttctg aaaaatattc aggatgtgtg gcaccaacta catacattaa
    18241 atttccggta ttcccccaag tggcagcctt gcgaatcaca tgttcataca gagcaccatc
    18301 gccatctgca attaattgga aatcttgcga acctttattg ctcgttaatg caagtaatat
    18361 cacccatttg tccgggtagg aaaaaaatgg cgtaacactg tcttctccca tgtatggggc
    18421 taccgtaatt gcatcgcacg acatagcacc gaaaaatgca tgtgcatatc gcgcactggt
    18481 attcccaata tcgccccgct tggcatctgc aataataaaa tgttctttgc cgatatattg
    18541 aattgttttc tccaaacttt cccagccttt cgaaccatac gcttcataaa atgccacatt
    18601 gggtttgtac gctacacaat gtgctcgcgt ggcatcaata atcgctttat taaacgcaaa
    18661 aatcggatcg ctttcttgca gtaaatgaac agggatcttt tcgagatcgg tatcgagtcc
    18721 gacacataaa aaactgcgct tcgttttaat ctgatgaatg agttgcgtgc gatccatgag
    18781 gcaaaattac tcaattagag gaaggccatg aaaggagaga ggaaaaagga aaaaggaaaa
    18841 aggagagagg agagaggaga ggggaatgtt tcagtctacg gtttctatgg ctttgatttc
    18901 ggggactgtt tttttgaggg ttgcttctac acccgctttc atagtcataa aactcattgg
    18961 gcaactttca caggcgccga ggagtttcac cttaacaacc atatcgtcgg tgatatctac
    19021 caccttgata tcgccaccat cctgattgag ataggggcgt atggagtcaa gagatgcctc
    19081 tacttgttga atcaatgcct cattaccgtt catagtgcaa aattagtcat tactaagcgc
    19141 attttcttat cgcgtgatct ctacaatttt agttgccgtt ttttcttcgt tgcgaattga
    19201 aatatagcgt gctgtgttat taatcaatgt atcaaaagct tctgttaaca caggtatatc
    19261 attgcgtaaa attgcaggtt tgccgctgtc cccaccttct ctaatccctt gaacaagagg
    19321 aatatgtgct aataatggca catcatactc tttagccaac tgtgtgccgc cgcctgtgcc
    19381 gaagatgaaa tatttatttt ccgggagttc ttcaggggta aaccaagcca tattctccac
    19441 tatacctatc accggaacat taatatttgg taatcggaac atacctactg ctttgcgcgc
    19501 atccgccagt gccacttcct gaggtgtggt gacaatcaca gcccctgtaa cgggcactgt
    19561 ttgcaccaac gtaagatgta tatctcccgt tcccggaggc agatctatga tcagatagtc
    19621 caattcaccc caaatacaat cggtaacaaa ttgtcgcaac gcactgctaa ccattggtcc
    19681 tcgccatact accgcctgtg tttcatcaac cagaaatcct atcgataagg ctttgatacc
    19741 atgtgcttcc agcggaatca tcatcggttt gccattcacc tcttcaactc gcggttgggc
    19801 atcttttagt cccatcatga ccggaattga aggtccatag atatcggcgt ccatgattcc
    19861 caccttggct ccggtttttg ccagtccgag tgccagattc accgaaacag tagattttcc
    19921 cacacccccc ttgcctgagc atacggcaat aatatttttt acacctggta agagttgttc
    19981 ctgatttcca cggatagacg acaccctcgc actcatgtta acgtcaacct ctgcctcctt
    20041 gcttaccagc agtttaacag cattgacaca tgccctgcga atctcctcct tcattgggca
    20101 ggcaggcgtt gtaagttcaa cggtgaagcg gatatgcgtg ccgtcgatgg ttatgtcttt
    20161 caccattccc aggctcacaa tatccttgtg aagatcgggg tcgtcgacgt tagaaagggc
    20221 ctttaaaata tctgcttctg aaatcgccat gccttaaaaa tttgggatgt aaagttacga
    20281 tgcagacaac aaaaagcctc ctttattagt ttgatatgcg agttgttaaa gtgcataaaa
    20341 agttgttttg ttgttcagaa aactatattt gcaccctaga caagtgctga catggggaaa
    20401 aatttgatga tcgttgagtc gccggcaaag gcgaaaacca ttgagaaatt cttaggaaaa
    20461 gatttcgtgg taaagtcttg ttatggccat atccgcgacc tggagaaagc cgattttggt
    20521 atagacttta agaataatta cgaaccgaaa tacattattt ctgccgataa ggaagctgtt
    20581 gttaaagagc tgaagaaatt ggcaaaaagc gcagaagaaa tatggttagc aacggatgaa
    20641 gaccgcgaag gagaagctat atcgtggcat ttgtgcgaag ttttgggctt aaatgccaag
    20701 gaagcaaaac gcatcgtttt tcacgagatt accaaaaaag ctattcaaaa cgctgttgcc
    20761 aatccacgta atattaatat agaccttgtc gacgctcaac aggcacgccg tattctggac
    20821 cgtattgtgg gttttgaatt gtcgccatta ttgtggcgta agataaacat gaagacttcg
    20881 ctttctgccg gtcgcgtgca atcggtagct gttcgtttga tcgttgagcg tgaacaggaa
    20941 atcgggaagt tcaatgcaat ggcatcattt aaggtgactg cattgtttca tgtgaaggat
    21001 gatagcggga agacttatca attaaaggct gaattacctg ccaagcttga tgaagaagcg
    21061 caggtgaaat catttttaca agactgcata ggtgctgaat ttcaagtggg gaatattaca
    21121 gtgaaacccg caaaacgctc ggcatcagcg cctttcacca catctaccct gcaacaggaa
    21181 gcgagtaata aattaggatt ttcagtatct cgcaccatga ttattgccca aaaattgtat
    21241 gaggaagggc atataaccta tatgcgtacc gactctgtca acctgagtca acaggccatt
    21301 gatgcggcgc aatctgagat cattaaaaat tatggcgaac gatattcaaa tcctcgtcaa
    21361 tacaaaacaa aatcaagtgg tgctcaggaa gcgcacgagg caattcgtcc aacgtacatg
    21421 gaaaatcaag atgtgccagg cgattttgac gaaaagcgat tatataaatt aatttggaag
    21481 cgcacaatgg catcgcaaat ggctgatgct gaattggagc gcacgatagt tgatattgat
    21541 atttcaacgc gcaaggaaaa acttgcagca aaaggtgagg tgataaagtt tgatggtttc
    21601 ctcaaagttt acactgaagc tttggaagat gagcgcgatg aagatgctac tatacttcct
    21661 ccattaaaag taggagcacc tctaaccatg attgaaatgg aaggtgcgca aaaattctct
    21721 aagccacctg cgcgatatac tgaagctagc ctggttaaaa agcttgaaga attaggtata
    21781 ggtcgaccat cgacatatgc atcgacaatt tctactgttc aaaagcgcga gtatgttgta
    21841 aaaaaagata aggaaggtat tgcccgcgaa gctgttcagt ttaaaataag tgaaaagcag
    21901 gttgtgcgca gtgtcgtaac agaaatgtat ggcgctgaac gcatgaaatt atttcctacg
    21961 gatctgggaa ttttagtgac tgaatttttg atagagaatt ttccgcagat attagattat
    22021 agttttactg ccgacgtaga agcgcagttc gatgatattg caataggcaa gcgcgagtgg
    22081 cataaaatga ttgatgcctt ttacaaacca tttcatgaac atctcgaact cacaaccaaa
    22141 acggcgcaac gcgttaccgg tgaacgcatt ttaggtgcac atccggaaac aggtgaacag
    22201 atttctgtgc gcatgggtaa attcggacct atggtagtga tgggtgaatt gctcgatccc
    22261 aaagaggcaa aggcaaaggg cgatggtaaa aaaccaaaat tcgcaggatt gctcaagcat
    22321 caaaatatgg ctgaaattac acttgaagat gccttgaaat tatttgaact acctaaaact
    22381 atcggtacat atcgcgagga tgatgtggtt gttggtgtag ggcgcttcgg tccatatgtc
    22441 cgcaatatga aaaagttcta ttctattcct aaagggacag aagccgctgc cattacactt
    22501 gaagaagcaa ttcagttgat taacgataag gacctgaagg atcaaaataa actcattcat
    22561 gattttcctg aacatgagat tcaggtattg aatggattat atggtcccta catcaaaaaa
    22621 ggcaaggata attataagat accgaaagat agggttgcaa aagatctgac attagaacag
    22681 atcctcgaga tcgttgaaac aacagaacct tccggtaagg gtaaacgcaa gtttgctaaa
    22741 aagaaatagc ctccatggag catcgtgaag cagaaataca agcgctgatc gcattgctgg
    22801 atgatagtga tcccgaagta ttcgaccatg tagctgcgcg attattatct tatgggccaa
    22861 tggttatcga taagcttgaa gacgcgtata ctaccattcc caatcctgtt atgcaggaaa
    22921 ggatagagga tattattcat caaattcaat ttgccggagt attagccgat atgcgcaatt
    22981 gggccgctca tggtcacaac gatctgatgc gagggttgct catcattacc cgtcaccaat
    23041 atcccgatct ggatgaagat gccatccttg caaatgtggc acgtatacaa aaggatatat
    23101 ggataggtat taatacctat ttatcgcctt tagaacaaat gaatgtgatc aatcaatctt
    23161 tgttttcaca ataccaattc gcggggttgc aaaataatga tgatgaactt cgatatgcat
    23221 atatcaacaa tatgctggat gcatttaaag ggaatcactt ttctgtaggc ttgctttatc
    23281 ttattctatg tcagcaactc gatatgcctg tttatggtgt attgttgagc acccatttta
    23341 tccttgctcg gatgaaagag gatattgtca attttgaagg tgataataaa gacgatattt
    23401 tattttatat caatccctac aacaaaggac tagcattcag tgagcgcgag ataaagggat
    23461 atctcagtaa gttggagata ccttctgacg aaaaatattt caagccggca tccaataagg
    23521 ctgtgttggc agagtatatc aggcatttga tttccttgat caaaaaacca caggaccaat
    23581 ataagatcga agacctcgag gcattgttgg catgtctgga atcggaataa ttcacgcgca
    23641 acattttaca catggcagaa aaggtaacca tcagcggatt tagctttgtg cgcaatgccg
    23701 atagattgtt ttatccggtg aaggaatcta tactttccat tttacctctc gttgatgaat
    23761 ttgtgattgc tgttggtgac aacgcgcccg gtgatggcac gattgcattg atcgaaagta
    23821 tacagtcgcc caagatcaag atattgcata ccgtttggga taccgaaaca tatcccaagg
    23881 caaccattta tgcccagcag accaatgtgg caatgaaagc gtgtaccggt gactggttgt
    23941 tttatatcca aggtgatgaa gttgtgcatg aaaaagacct ggatgccatc agaaagcagt
    24001 gcgagaaata tgctgatgat ccggaggtgg aagggttatt gttcgactat tatcatttct
    24061 gggctgatta tgcgcatatc aacagatcgc acagcgcgta tgcacaggaa atacgcatgg
    24121 tgaagaatcg caaggatatt ttttcctatg gtgatgcaca gggatttcgc aggataccgg
    24181 gatttgatat gcgcaatttc agacagaagg aaaacgtatt taaattaaaa gtggctccgg
    24241 cacaagcgca catttatcac tacggctggg tgcgaccacc ggattttatg atgcgcaaga
    24301 agcagtttgc caatacactg catcacgggc aagcggaaac agatgcaaac tacaaagcgg
    24361 cgatgtttga ttacggcccg gttggtagga aatttccctt taaggacacg catcctgccg
    24421 taatggccga acgtgtgaa
//
LOCUS       DAOQOU010000034        23404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1039_length_23404_cov_6.169129, whole genome shotgun sequence.
ACCESSION   DAOQOU010000034 DAOQOU010000000
VERSION     DAOQOU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23404
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_23404_cov_6.169129"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..2466)
                     /locus_tag="PLM90_04825"
     CDS             complement(139..2466)
                     /locus_tag="PLM90_04825"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88723.1"
                     /translation="MLRKSILSLTLICLGFLVRGQQYTVSGYVTDASSGEHLISATII
                     DKRDYAGTIANNYGFYSLTISGGDAQIVCSYIGYTTITKNIHLVSDTVIHFQLKPDLE
                     LETVVVTADNEDKLQDRTQMSTMEVPIEQIKALPAFLGEVDVLKTLQLLPGVSGGSEG
                     TSGLFVRGGSPDQNLILLDGVPVYNASHLFGFFSVFNSDAINKVTLVKGGFPARYGGR
                     LSSVVDIRMKEGNMQTFHGEGSIGIIASRLTLEGPIIKDKTSFIVSARRTYIDLITRP
                     IFALQDDYSGQQGYYFYDLNAKINHKFSDRDRLYVSGYFGRDKAYFHNDYQYDAETNR
                     SEESGISWGNATAVARWNHMYSKKTFGNLTATFTDYTFDIFSNFTDERENDPDTYFGF
                     EYFSGINDWSLKYDVDYIPNPMHYLKYGGGYTYHTFRPGATQFETNEPGTEDFNLELG
                     APNIFAHEFDIYGEDDYEISDVFKVNYGLHASAFLVNEKYYGSIQPRISGRYLLNPDL
                     SLKASFSTMTQYIHLLSNSGIGLPTDLWVPATDSVPPQKAYQPAIGIAYTFKQQYEIS
                     VEGYYKYMKDIIEYKDGTNYLVDGLDGWEDKVESGEGWAYGAELFIQKRSGKVTGWIG
                     YTLSWSNRQFPTINLGEKFPYKYDRRNDFEIACIYKFNDHIDFSGTWVYSTGQPISLP
                     IAKYEGMFNETIYAYEGRNGYRMNAYHRLDLNVSFHKEKEKYERTWNIGAYNAYSRKN
                     PFFIYLEYDYLTNTNNYKQVSLFPIIPNVSWEFKF"
     gene            complement(2568..3743)
                     /locus_tag="PLM90_04830"
     CDS             complement(2568..3743)
                     /locus_tag="PLM90_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88724.1"
                     /translation="MKRMIYALCLFTLLASGCKVAEKEFRKGNYDDAIDICVKRLIDN
                     PDKAEYIQILEESFRRANDGDLAAIKALHTDGNPDRWSTVYDIYQGIYVRQEKIKPLM
                     PLYLGTEERDAEFKFVDAVGELNNAKKNAAAYWYALGDEKMKSGDMHQARDAYYEYEK
                     IYRYYSTYKDLDAKIAAAREKGMAQALFVLTNQSSGNYNYDVQQAINRFKVYEDNGNW
                     FRLYNAYDPKEADFTVELVVRTIEAYPDKVTTNRYEETKEIQDGFEFLYDAQGHIVKD
                     SLGNPIKVPDYKTVSVFVTETWQEKIATMSGEILYKDANGRVMRSIPVRSDAVFQNYY
                     ATATGYYEALSAQSKQKIGGQPIPFPSENTLLLDAVKQMECNVNDVLEDYNDEYLNR"
     gene            4037..5290
                     /locus_tag="PLM90_04835"
     CDS             4037..5290
                     /locus_tag="PLM90_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ88725.1"
                     /translation="MKKQTTIRRLLTGFLFFAFLTTANQAFAGEAEGQTLFQTNCQRC
                     HLIDKKMTGPALMDVQSRWTDSTHLYAWIRNSQAFLATGDKYANDLYKQYNSIMPAFP
                     QFKDQDIADILYYIKVEGERKKAEAANTTTGGPVTTTAKNDNSTLYWILIIALVFIAF
                     ILARAVRYLDGLVRQKYDEGEADRTPFWRQRKYRPLVALLVLAFLCLLSVAMWNSATS
                     LGRQQGYAPEQPIAYSHKVHAGINKIDCKYCHVGAERGKSAVIPSLSVCMNCHYNIQG
                     VTGKDPNYPKEVYNKEIAKIYSYIGFNPETNTYDKEAKPIEWVRIHNLPDHVYFNHAQ
                     HVTVAGIECQTCHGQIQEMDVVQQQENLSMGWCVNCHRNSNVNFTTNDFYKPYEVLHE
                     KLKNGEIKDVKAVDIGATECQKCHY"
     gene            5326..8463
                     /locus_tag="PLM90_04840"
     CDS             5326..8463
                     /locus_tag="PLM90_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HMZ88726.1"
                     /translation="MAEKKYWKGLEELKQEPEFMQLTQHEFHQEIPVEFFDEQNDTLQ
                     SSRRDFLKIMGFSLTAATVAAGCEMPVRKVAPYIFKPEDVIPGIATWYASTYYHGGDY
                     CSILVKTREGRPIKVEGNRDSLITKGGTSARVQGSVLSLYDTARYRNPMVKGSRGMGN
                     ATWDEVDKAIMGKLEANPNAQIRIVTSTVISPSTKKVFAKFAEKYPNTKVYAYDAISY
                     DGMLMANMATKGKAEIPHYNFSKAKAIVGIDADFLGTWISPVEYTKGYVANRKVSKGN
                     TDMSRHYQFESRLSLTGSNADYRVPVLPSELGIVALNLYDAITGGNASNGVKIQDAEK
                     QKRIAEAAAYLKANRGESLVVSGCNDKNVQVVINAINDALGNYGTTISFDGASQLRQG
                     TDASIKTLISELNGNQVTAVMFYDCNPVYDHAMGAELKEAIGKAAISVSFATAINETA
                     EACGIISPDHHYLESWNDFEPRKGFYSLSQPTIHNLFETRQMQDSLLKWSGNNIGYYD
                     FMMQAWAEGPLAGSMSTTTAFNKALQIGLYQPESHRGMEGAGDIVKAMLAADAVMANE
                     APVADLSSSASAIIAMSKKASGLQVQFYETVAIGDGRYADNPWLHEMPDPISKITWDN
                     YISVSPKYASEKGLMHGDMVTFSVNGKSVKLPVAIQPGQAYGSVSIALGYGRTAIGKG
                     GAGVGVNVYPMLDASGDCVSYMASNATMEPVGENVKLAQTQTHFSINDGLSVRRIIKE
                     TTLAEYKKSAKAGNEDREEALAWNNVTFYPDYFGKKNGFNWGMNIDLNSCIGCGACVV
                     ACNAENNIPVVGKKEVIRAHEMHWMRIDRYYGGDPENPEVIYQPMLCQHCENAPCENV
                     CPVGATNHSSEGFNQMAYNRCIGTRYCANNCPYKVRRFNWFDYGSADTFGTMNDPSGT
                     ENLGLFEDLTRLVLNPDVTVRSRGVIEKCSFCVQRIQDAKLTAKKEGRELADGEIRTA
                     CQSGCPADAIVFGNNYDEKSAITTETMDERTFGILEEFHWLPSVTYKTKVRNKDKADS
                     HGTEALDIMDLFNYK"
     gene            8483..9883
                     /gene="nrfD"
                     /locus_tag="PLM90_04845"
     CDS             8483..9883
                     /gene="nrfD"
                     /locus_tag="PLM90_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HMZ88727.1"
                     /translation="MFVSPLREPLIQGHKTYKQISDDICAPVEAKPSKAWFIATTLSG
                     LLAINGFVMIGLTITYGIGMWGLNKTVGWAWDITNFVWWIGIGHAGTLISAILLLFRQ
                     KWRTGVNRSAEAMTIFAVICAGLFPIIHMGRVWLAFFIFPYPNTRGPVWVNFNSPLLW
                     DVFAISTYFTVSLVFWYTGLIPDLALIRDRAKSAFTKKMYNIACFGWKGTAKNWQRFE
                     SISLVLAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVLTLM
                     LVVRKVFNLQEYITLAHIEAMNKIIILTGSIVGVAYITELFTAWYSGNPYERYAFLNR
                     AMGPYWWSYWGMMTCNVVSPQLFWFRKLRRSVVFTFFMSIFINVGMWFERFVIIVTST
                     HRDFIPSNWTMYYPTYVEMFIYIGTIGFFMTLFLIFSKVAPVIAVAEVKSILKTAGDQ
                     YTGPDAIHKHEEDNHH"
     gene            9915..10442
                     /locus_tag="PLM90_04850"
     CDS             9915..10442
                     /locus_tag="PLM90_04850"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HMZ88728.1"
                     /translation="MATKKIMYGLYDDEVVLIKAVKKVRAEGLKIHDCYTPFPVHGLD
                     DAMGLRMTKLHSAGFIFGATGTLTALSFILWITTNNYPINYGGKPYLSLPSWIPITFE
                     LTVLFASVGMFLTYLYLNRLAPGMKAEIVDERTTCHMFAMTFEIDDHTTDAKKTEIRK
                     VLSDSGAVEIHEKEL"
     gene            10461..11129
                     /locus_tag="PLM90_04855"
     CDS             10461..11129
                     /locus_tag="PLM90_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ88729.1"
                     /translation="MVKRNIMIALVAAVMIPAVLTSCKKASGDYPGDSYTWDMSYSRA
                     YEPYTLNPELRDSMTAMTPVAGTVPYVGNAVAGVKADSLKMALNLPYTIPNTPEGYLR
                     AATENHNPFSINDEKVVAQGQHFFNIYCAVCHGVEGNGKGFIVTEGKYTAMPPSYFDP
                     MYMAMTEGTMFHSVTYGRNAMQSYAYALSKEERWKVITYVKKMQADYAAQNAATAATT
                     TGTN"
     gene            11172..12560
                     /locus_tag="PLM90_04860"
     CDS             11172..12560
                     /locus_tag="PLM90_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88730.1"
                     /translation="MNGSNKFEYTAKLKNRFLGMLVIGVVLVVVGMLTGASMDRFWSN
                     YLQNTLFFLGISVLAVFFLAAHQIGMAGWHITVKRIPEAMSQFSKVGAVLMLVVVFGV
                     IFDYHHLYHHWTNTFIQEKEVSVEALKDYEAELAEEGHSTEHAVDYNRTWQDFQYHQV
                     APAPEHVDAAKPEPEVLENGMVVNPYYDRLIDHKSAYLNKTFWSIRSISYLFLWVLMA
                     MIIRRYSLREDLDNSDTFYQKTRKAAAVFLIIWAVSSSTMTWDWVMALDPHWYSTLFG
                     WYNFISLFVASICTTILILIYLKRNGYLTQLNENHLHDLGKYAFGFSVFWTYLWFSQF
                     MLYWYGNIPEETRWCLDRARTDYKWLFYANLLLNFLFPFLTLMRRDAKRNMNVLMIVA
                     IGLIITHWLDFYMMITPATLGTEVHFGILEIGMFIAYAGLFLFVVFGELAKASLIPQN
                     HPMYKESLDHHI"
     gene            12576..13994
                     /locus_tag="PLM90_04865"
     CDS             12576..13994
                     /locus_tag="PLM90_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HMZ88731.1"
                     /translation="MATGWLIFIAILMIVVVLFQVTRTLDLVGQLRGGDTTTVSMTKF
                     HASLGMIFMVLGMIGFFWSFGHFHDYLVDHHSSDNGLIIHRMFVITLWVTGIVFVITN
                     VLLFIFVYQYRHRPGHQATHFAHSNKLEFIWTVVPTIVLTGLVVFGLQAWTDIMGDAE
                     DDAIVFEVTGQQFFWSARYPGPDGKLGPRDNDLICADNPLGIVTREWIDHRRVTLIGS
                     TKLGVKGEIEKLQDREKEINAIIASYEQRIATSPNKYKVADAKDSLEIVKKELGKIPG
                     KIEIREKALTRINKKYTEEYINQHAAEFTWGYDDPMPSEVHVPVDRQAFVKITALDVL
                     HNFYIPHMDVKMDAVPGMPTSFKFKPLITTADKRIEMSENPLWQVTKEGEKEPRWKNF
                     NYEIACAELCGSGHSAMKYTLVVETQEDYDKWISAQQSKWSQVAASLKLENGFSKFAP
                     KLEAPAAETPAAPADTTMITKK"
     gene            14016..15890
                     /locus_tag="PLM90_04870"
     CDS             14016..15890
                     /locus_tag="PLM90_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ88732.1"
                     /translation="MSEHHAVAHHTEAHAHGHDHHGHDGHHELGFIRKYIFSEDHKTI
                     AKQFLVTGIFWAIIGALLSVLFRIQLGYPNADLTWMKPFLGKWITEEGQIAPEFYNAL
                     ITMHGTILVFFVLTAGLSGTFSNFLIPLMVGARDMASGFINMLSYWFFFISGAVMFVS
                     FFLATGPASGGWTAYPPLNALHDASSGGVLGVDYWLISMALFIVSALLGGLNYITTVI
                     NLRTKGMKMFRLPLPIWAFFLTAVIGVLSFPVLLSAAILLLFDRNLGTSFYLDAINLA
                     GEPLANSGGSPILYQHLFWFLGHPEVYIIILPAMGIVSEVLSVHSRKPIFGYTAMIYS
                     LLAITVLSFIVWAHHMFMTGMNPFIGSIFVIFTLIIAVPSAIKVFNWLTTLWKGDIRL
                     NPQMLFAIGFVSLFISGGLTGIFLGNSSIDIRLHDTYFVVAHFHLVMGVAAFFGMFAG
                     VYHWFPKMFGRYLNPTLGYIHFWGTISAAYLIFYPMHFEISLPRRYYTHSGFETFSDF
                     AHLNAFISTVAIISFALQLLFVFNFFYSIWYGRKLRESNPYGANTLEWTTPLRPGHGN
                     WEGDIPEVHRWPYDYSKHGRDFIPQTEPLSQYEIEHGEHGDEGDRDIIKKEEDENHKG
                     "
     gene            15898..16791
                     /gene="cyoE"
                     /locus_tag="PLM90_04875"
     CDS             15898..16791
                     /gene="cyoE"
                     /locus_tag="PLM90_04875"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HMZ88733.1"
                     /translation="MRKDSSIAGSGIGKIFAARLKDYHMLVKFRLSFIVVFSSGIGYL
                     FCGGEQSQFFLFLLGGFLITAASNAINQIIESDTDQLMTRTADRPLAAGRMAHTEAII
                     AAGVMAVSGIALLWWQFNSLSALLGALSLISYAFIYTPMKKVSAIAVFIGAFPGAMPP
                     LIGGAAANGEISNIAILLFCVQFVWQFPHFWSIAWVACEDYAKAGFHLLPTASGRSKA
                     SAVHILLYAALLIPVSLLTYFLGYTGTVSAVIVAVTGLLFFLQAVHLFRTADTKAARS
                     LMFGSFLYLPIVLIALLVDQI"
     gene            16798..17382
                     /locus_tag="PLM90_04880"
     CDS             16798..17382
                     /locus_tag="PLM90_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HMZ88734.1"
                     /translation="MAVSIHKRFIVHPYKFNMWIAIVSMVMIFAAFTSAYVVKKGDTK
                     TWADIQLPQIFTLSTIVIVISSLLMHAAYVSFKKGALEWYRGFMTLTFLAGAAFLSMQ
                     IQGWYTLVAQGVYLTDNVAGSFIYVISGAHFLHVAGGVIALLVFSIRSFTRYQASNIE
                     AMKDTTINVEVMATYWHFVDILWVYLFFFFMLNR"
     gene            17395..18069
                     /locus_tag="PLM90_04885"
     CDS             17395..18069
                     /locus_tag="PLM90_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMZ88735.1"
                     /translation="MEAVATTNAKDLWSGGDSPFKVSYGKIMMWYFLISDTFTFAGLL
                     ISYGAIRTSQPKHDAANPLSWPDPNEVFNAGPGFHNAPLIFVTFMTFTLILSSVTMVR
                     AVQEGHQMNRSGVLKWMAFTILGGLVFVGSQAWEWSHLIEEGMTPHGNPFGASSFGAL
                     FFLITGFHGFHVSVGVLLNSWAFISTYRGVFDRRGHYEMIEKLGLYWHFVDLVWVYVF
                     LAFYLF"
     gene            18089..18424
                     /locus_tag="PLM90_04890"
     CDS             18089..18424
                     /locus_tag="PLM90_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HMZ88736.1"
                     /translation="MSTLTQEESRKRVWRVCGILAVVTAVEIAAALIHYNYMHDSPKS
                     FLNILFVVLSAAKAYYIMSEFMHLKYELKPMVISILAPFLFLVWGIIAFLWEGHYWLS
                     EKELWHILK"
     gene            18426..19082
                     /locus_tag="PLM90_04895"
     CDS             18426..19082
                     /locus_tag="PLM90_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ88737.1"
                     /translation="MSSTRGRIWTIVMLVIILVLPSIAYLMLREGLHVRESAPASDHL
                     IKENLQSIPTYSGVSFRGDTISKSRMKGKVCILNFTTHACEKSMDDNMRKLFEIQEDY
                     YGKTISFRIITNTLDPDTDGLDNMRLMSERYAGREIWHFITDPDSSGVKLYDWCKKNA
                     TDSTLNETDTQCPQLVYLVDGNGKLRGVYNILEEQQFHDLYNDILYLVNQMELHEQKK
                     "
     gene            19066..19719
                     /locus_tag="PLM90_04900"
     CDS             19066..19719
                     /locus_tag="PLM90_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ88738.1"
                     /translation="MSKRNNLIIFGVSGVAFILFVIFVYLPYAKKQDNLNKVKESYQH
                     SSYLKDPVGQLPVFSCIDQNGKVFTNDSLKGDVVVANFFYTTCEGYCPTTTKHLATVQ
                     SSLNKNIPFHIISFSLNPANDTVAALRKYADMYKADDNVWRFLHGDQEEIYQLGEQGL
                     MTIVKDTAGNFESHSDRFVLMDKMGKIRGFYRGTDSLELQALIQDINYLVFKGETNE"
     gene            19712..20272
                     /locus_tag="PLM90_04905"
     CDS             19712..20272
                     /locus_tag="PLM90_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HMZ88739.1"
                     /translation="MNERTINRWIVAISILIPVAVTILFYMPAVKLNIDTSILPAFHA
                     MLNSGVAILLLLGLYFIRTGKRGAHRTSMLGAFSLSALFLISYVLYHLTSDSTLFGDL
                     DHNGIVSDAEKAEAGPMRGVYLVILLSHIFLAALIMPFILVTLSRALTARFDKHRKIA
                     RITWPLWLYVAITGVVVYFMIAPYYP"
     gene            20297..20551
                     /locus_tag="PLM90_04910"
     CDS             20297..20551
                     /locus_tag="PLM90_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88740.1"
                     /translation="MKYRLLIMLVLVVLLSALHTDLQAQCAMCKATAEEGLREGNTQS
                     ANLNAGIMYLLAMPYLAFGVIGFLWYRNYKRKKAQEAAGL"
     gene            complement(20552..21253)
                     /locus_tag="PLM90_04915"
     CDS             complement(20552..21253)
                     /locus_tag="PLM90_04915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ88741.1"
                     /translation="MNCIITGGTKGIGKACALLFAKNGFNVAICARDKAALNEMSAQL
                     HDANPTGKHLAISCDVRDRAQLHSFGENCLKAYGSIDVLINNAGVFLPGNITEEAEGT
                     LEFLIETNVYSAYHLTRQIVPVMRTAKKGYIFNICSVASINSYDGGGSYAISKFALLG
                     FTKNLRHELKTDNIRVTAVLPGAVLTPSWDGTPLPESRFIPADDIATLILQAYQLSDR
                     TVVEEILIRPQLGDL"
     gene            21298..>23404
                     /gene="priA"
                     /locus_tag="PLM90_04920"
     CDS             21298..>23404
                     /gene="priA"
                     /locus_tag="PLM90_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HMZ88742.1"
                     /translation="MRSVFVDVILPFALEKNYTYAVPDEFVGVLSPGMRVEVPFRNKT
                     YTGIVAKVHDISPQGYSPKPVLSLPDHLQVVNKTQLDLWTWISKYYMCSVGDVMNAAL
                     PAPYKLSSETVVVYNPQHGIDLLTLDEKEYLIAEALTLQQELRLIDVQQILRQKTVQP
                     IIKGLIAKGIAFIKEELKESYTVKTEKYIILTSQYRSEDALQQLFESLQKSEKQTNVL
                     MIYYQLSAKEGKVKKSVLLRRSGVTAAVLKTMIKNDIFEEVEEQVSRLQAYGNADMHD
                     AHLNADQQKALSEIEEQFEEKQTVLLHGVTGSGKTEVYIELMLKTIAAGKQVLYLLPE
                     IALTAQIVTRLKKRFGNLIGVYHSKFNQNERIEIWQHVLNNEFKVILGARSALFLPFN
                     EIGLIIVDEEHDYSYKQHEPSPRYHARDTAIYLGHLHQAKVLLGTATPSVETYYQAKS
                     GKFGLVEMFRRHGDMPMPTIQIVDMKAEEKQKTVQGHFSSVLVGNIKHALQLKEQVIL
                     FQNRRGYAPFMICQSCGWTPKCTQCDVNLVYHKYANELRCHYCNHVENTYSVCPACNS
                     SQLIIKGFGTERIDDDLQYTFPDARTARLDLDAIKTKHGHEKLVRSFEEGEIDILTGT
                     QMVTKGLDFDHVNLVGILSADQIINFSDFRAAERAFQMITQVSGRAGRKHKKGLVLIQ
                     AIKVDHPVLQYVITNDFHGF"
BASE COUNT         6622 a         4848 c         5031 g         6903 t
ORIGIN      
        1 ttatcttgtt atcgtgtcct gtaaaaaaaa cctcaagccc tctctccttt ctcctctttc
       61 ctctctcctt tctcctttct cctttctcct ctttcctttc tcctctttcc tttctcctct
      121 ttcctttctc ctctttccct aaaatttaaa ttcccatgaa acatttggaa taattgggaa
      181 caaactcacc tgcttataat tattcgtatt cgtcaaataa tcatactcca gataaatgaa
      241 gaagggattt tttctgctat aagcattgta tgcgccaata ttccatgtgc gctcatattt
      301 ttctttctct ttatgaaaag atacgtttaa atccagtcga tgatatgcat tcattctata
      361 tccatttcta ccttcatacg catatatcgt ctcattaaac atgccttcat atttcgcaat
      421 cggcaaactg attggttgcc cggtgctata aacccatgtt cccgagaaat ctatatggtc
      481 gttgaattta taaatacatg caatctcaaa atcattgcgc ctgtcgtact tatatggaaa
      541 tttttctccc agattgattg ttgggaattg cctgttgctc cacgacaacg tatatcctat
      601 ccatcctgtc acttttccgc tgcgcttttg aataaataat tcagcgccat aggcccagcc
      661 ctctcctgat tccactttat cttcccagcc atccaagcca tcaactaaat aattggtgcc
      721 atccttgtat tcaatgatat ctttcatgta tttataatat ccttccacac taatctcata
      781 ttgttgttta aatgtgtagg ctattccaat tgcaggttga tatgcttttt gcggaggaac
      841 actatccgtc gcaggaaccc acagatctgt tggtaacccg atgccggagt ttgataacag
      901 gtgaatatat tgcgtcatgg tactgaatga tgccttcagc gaaaggtctg gatttaataa
      961 atatctgcca ctgatgcgcg gttgtatgct gccgtaatat ttttcattta ccagaaatgc
     1021 tgatgcgtgt aatccgtagt tcactttaaa gacatcactt atctcataat cgtcttcacc
     1081 atagatatca aattcatgtg caaaaatatt tggtgcgcca agctcaagat tgaaatcttc
     1141 tgttccgggc tcattggttt caaattgtgt ggctccggga cgaaaagtat ggtatgtgta
     1201 tcctcctcca tattttaaat aatgcatggg gtttggaata taatcgacgt cgtattttaa
     1261 actccaatca ttgatgcctg aaaaatattc gaatccaaaa tacgtgtccg gatcattttc
     1321 gcgctcatct gtgaaattgc tgaaaatatc aaaggtgtaa tccgtgaaag ttgccgttaa
     1381 attgccaaaa gtcttttttg aatacatatg attccatcgt gcaacagcag tggcattacc
     1441 ccacgaaatg ccactttctt ccgaccgatt tgtttccgca tcatattgat aatcattatg
     1501 aaaataggcc ttatcgcgac caaaataacc actgacatac aatctgtcgc ggtctgaaaa
     1561 cttgtgatta atcttggcat ttaaatcgta gaaataatag ccttgttgcc ctgaataatc
     1621 gtcttgtaat gcaaaaatcg gccttgtgat aagatcgata tatgttcgtc ttgctgaaac
     1681 tataaaggag gttttgtctt ttataatagg tccttccaat gttaacctcg atgcaataat
     1741 gcctatgctc ccttcaccat gaaatgtttg catattgcct tccttcattc tgatatctac
     1801 aacacttgat aaccttccgc cataccgtgc aggaaatccg cctttaacaa gagttacctt
     1861 gttgatggca tctgaattaa atacactgaa gaatccaaaa agatgtgatg cattatacac
     1921 aggcacacca tctaacaaaa tcaaattctg atcaggagaa ccaccgcgaa caaataaacc
     1981 gctcgtgccc tctgaaccac ctgaaacacc gggcaataat tgcagggttt ttaatacatc
     2041 tacttcacca agaaaggcag gtaatgcttt tatctgttct attggtacct ccatcgtact
     2101 catctgcgtg cgatcctgaa gtttgtcttc attgtctgcg gtaactacta ctgtctctaa
     2161 ttccaaatca ggttttaact gaaagtgtat caccgtatct gaaacgaggt gtatattttt
     2221 cgtaatagtg gtatacccga tgtaactaca aacaatttgc gcatcaccgc cggatatcgt
     2281 gagactgtaa aacccataat tattagctat agtaccggca taatcacgct tatcgataat
     2341 ggttgcgctg atcaaatgct cgcccgatga ggcatctgtt acataaccac taactgtata
     2401 ttgctgaccg cggaccagaa atcctaaaca tatcagggtc agactcaata ttgattttct
     2461 taacataatt aaaataaatg cgggtgaaaa tacgcaaatg ccccgatttg ccctttcacc
     2521 cgcaggtata tgattgtttt ttgacaagtg ttaatttgat agcatactta cctgttcaag
     2581 tattcatcgt tataatcctc cagcacatca ttgacattac attccatctg cttaacggca
     2641 tccagcaata aagtattttc actcgggaat ggaatgggct gaccgccaat tttttgtttc
     2701 gactgcgcac tgagggcttc ataatacccg gttgcagtag cataataatt ttggaacaca
     2761 gcatcgctgc gaacaggaat ggagcgcata actcttccgt ttgcatcctt atacaatatc
     2821 tcaccgctca tggtggcgat tttttcctgc catgtctctg taacaaatac agaaactgtt
     2881 ttgtaatcag gcacctttat cgggttgcca aggctatctt taacaatatg accctgagcg
     2941 tcatacagga attcaaagcc gtcctgtatc tccttggttt cttcatagcg gttagtggtc
     3001 accttgtcgg ggtatgcctc tatggtgcgc accaccagtt ctacagtgaa atccgcttct
     3061 ttagggtcat aggcattata cagcctgaac caattgccat tgtcctcata caccttgaaa
     3121 cggttgatgg cttgctgtac atcgtagtta taattgcctg aactctggtt ggtgagcaca
     3181 aacaatgcct gagccattcc cttttctctg gctgcggcga tctttgcatc caaatcttta
     3241 taggtagagt agtaacgata tattttttca tactcatagt aggcatcgcg cgcctgatgc
     3301 atatcaccgg atttcatctt ttcatcacca agtgcatacc aataggcagc ggcgttcttt
     3361 ttcgcattgt taagctctcc gacggcatct acaaacttaa actccgcatc gcgctcctcg
     3421 gtgccaagat ataacggcat caagggcttt atcttctcct gacgcacata aatcccttgg
     3481 tagatgtcat aaacggtcga ccacctgtcg ggattaccgt cggtatgtaa agctttaata
     3541 gccgccagat caccatcatt ggcgcgacgg aacgactctt ccaggatttg aatgtactcc
     3601 gccttgtcgg gattgtcgat caggcgtttt acacaaatgt cgatggcatc atcgtaattc
     3661 ccctttctga attctttctc tgcaactttg catccactgg ctaacagggt aaataagcac
     3721 aatgcgtaaa tcattctttt cataaatcca ttgattttca attactcaca tacaaaactg
     3781 tgccagtgtt gtcgctttac caaaccttta cgtaacccac aggtatgtgt ataactcaca
     3841 atgttaattt agaacacgtc taaacaagct atttcatctt cgtgtaaccc ttatgcagca
     3901 aggatttgag catgacgaaa taaggtcgaa aaaattttaa aagatttgtt aaagatttga
     3961 agcttggtgg ttagctttgc acgggttttt agacaacagc catattttaa ccaattaaat
     4021 atactctatt gtgcgaatga agaaacagac gaccatcaga cgcctactga ccggtttctt
     4081 atttttcgcg tttctgacaa cagccaatca ggcgtttgca ggcgaagcag aaggacaaac
     4141 attgttccaa accaattgtc aacgctgtca cctcatcgat aaaaagatga ctggaccggc
     4201 attgatggac gttcaatcgc gttggaccga cagcacccac ctgtatgcat ggatcagaaa
     4261 ttcacaggct ttcctggcta caggcgataa atatgctaac gatttgtaca aacagtacaa
     4321 cagcatcatg cctgctttcc cgcagtttaa ggatcaggat attgcagaca tcctctacta
     4381 catcaaagta gaaggtgaac gcaagaaggc agaagcagcc aacacaacaa ccggcggacc
     4441 ggttactacc acagcaaaga atgataactc taccctttat tggatattga tcattgcgct
     4501 ggtatttatt gcattcatct tggcacgtgc cgttcgttat ctcgatggtc tcgtgcgtca
     4561 gaaatatgac gaaggtgaag ctgatcgcac tccgttctgg cgtcaacgga aatatcgccc
     4621 actggttgca ttattggttt tggctttcct atgtctgctc agcgttgcca tgtggaacag
     4681 tgctacatcg ctcggccgtc agcaaggtta tgctcctgag caacctattg cttactcaca
     4741 taaagtacat gcgggcatca ataagattga ctgtaaatat tgtcacgtag gtgccgaacg
     4801 cggaaaatct gctgtgatac catcattgag tgtttgtatg aattgtcatt ataacattca
     4861 gggagtaaca ggaaaagatc caaactatcc gaaagaagtt tataataaag agattgctaa
     4921 gatctatagc tatatcggtt tcaatcctga aacaaatact tacgataaag aagctaaacc
     4981 gatcgagtgg gtgcgtattc acaacctgcc tgatcacgta tatttcaacc acgcacagca
     5041 cgtaacagtt gccggcatcg aatgtcagac ctgtcacggc caaatccagg aaatggatgt
     5101 ggttcaacaa caggaaaacc tgtcgatggg atggtgcgtg aattgtcacc gtaattcaaa
     5161 cgtcaacttt actaccaacg atttctataa gccttacgag gtgctgcacg aaaaacttaa
     5221 aaacggcgaa atcaaggatg ttaaagctgt agatatcggc gctactgaat gtcagaaatg
     5281 ccactattaa ttttattcta ataacccata tctaaccata accagatggc agaaaaaaaa
     5341 tactggaaag gccttgaaga gttaaaacaa gagccggaat tcatgcaact tacgcaacat
     5401 gaatttcacc aggaaatacc ggttgagttc ttcgatgaac aaaacgacac gctgcagtct
     5461 tcgcgtcgcg acttccttaa aataatggga ttcagtctca ccgcagctac cgtagcagcg
     5521 ggatgcgaaa tgcctgtgcg caaagtggca ccgtatattt tcaaacctga ggatgtaatt
     5581 ccgggtatcg caacttggta tgcttctact tattatcacg gcggcgacta ttgcagtatt
     5641 ctcgtgaaaa ctcgtgaagg tcgcccaatc aaagttgaag gtaaccgcga ttctctgatc
     5701 actaaaggtg gtacatcagc acgcgtgcaa ggttctgttc tgagccttta cgatactgcc
     5761 cgctaccgca atccaatggt gaaaggctca cgtggcatgg gaaatgctac ttgggacgaa
     5821 gtagataaag ccatcatggg taaattagag gcgaatccaa atgcgcaaat tcgcatcgtt
     5881 acttctacag ttatcagccc ttctacaaaa aaggtgtttg ccaaatttgc tgagaaatat
     5941 cctaatacga aagtatatgc ttacgatgcc atttcttatg atggtatgtt gatggcaaac
     6001 atggcaacaa aaggtaaagc agaaatacct cattataatt tttcaaaagc aaaagctatc
     6061 gtaggtatcg atgccgattt cctcggaaca tggatatctc ctgtagaata taccaaaggg
     6121 tatgtggcaa accgcaaagt atcgaaagga aataccgata tgagccgtca ctaccaattt
     6181 gaaagccgtc tgtcactcac cggttctaat gccgattatc gagtgccggt attgccttca
     6241 gaattaggta tcgttgcact taatttatat gatgccatca ccggcggaaa tgcttctaat
     6301 ggtgttaaaa ttcaggatgc cgaaaaacaa aaacgcattg ctgaagctgc agcatatctg
     6361 aaagctaatc gcggcgaatc actcgtagta agcggttgca atgataaaaa tgtacaagtt
     6421 gtaattaatg ctattaacga tgcattagga aactatggta ctaccatcag cttcgacggt
     6481 gcttcacaac ttcgtcaagg tactgacgca agcatcaaaa cactgatcag cgaattgaat
     6541 ggtaatcaag ttactgccgt aatgttttac gattgtaatc ctgtgtatga ccatgcaatg
     6601 ggtgctgaat taaaagaagc tatcggcaaa gcagctattt ctgtttcttt cgctactgca
     6661 atcaacgaaa ctgctgaagc ttgcggaatc atttcgcctg atcatcatta tcttgaatca
     6721 tggaatgact tcgaaccacg taaaggtttc tattcactca gtcaacctac tatccataat
     6781 ctgttcgaaa cccgtcagat gcaggattca ttgttgaaat ggagtggtaa taatattggt
     6841 tattatgact ttatgatgca agcctgggct gaaggtccat tggctggctc tatgtcaact
     6901 accactgcat tcaacaaagc gttgcagatc ggtttatatc aaccggaatc acatcgcgga
     6961 atggaaggtg ccggcgatat cgtaaaagca atgctcgctg ctgatgcagt aatggcgaat
     7021 gaagcacctg ttgccgactt atcttcttct gcatctgcaa tcatcgcaat gtcgaaaaaa
     7081 gcttcgggtt tgcaagtgca gttttatgag actgtagcta tcggcgacgg tcgttatgcc
     7141 gacaatccat ggttgcatga aatgccggat ccgatatcaa aaatcacttg ggataattat
     7201 atcagcgtat cgccaaaata tgcaagcgaa aaaggactta tgcatggcga tatggttaca
     7261 ttcagcgtaa acggtaaatc agtgaaatta cctgtggcta ttcagccggg acaggcttat
     7321 ggatctgttt ctatcgcgct tggatatggc cgcacagcta tcggaaaagg tggtgcaggt
     7381 gttggtgtga atgtttatcc tatgctcgat gcatcaggtg attgtgttag ctatatggca
     7441 tctaatgcta cgatggaacc tgtaggtgaa aacgtaaaac ttgcacaaac acaaacgcat
     7501 ttcagcatca acgacggact ttctgttcgc cgtatcatca aagaaactac ccttgcagaa
     7561 tataagaaga gtgctaaagc cggtaacgaa gaccgcgaag aagcacttgc ctggaataat
     7621 gtaaccttct atcccgatta tttcggtaaa aagaatggtt tcaactgggg tatgaatatc
     7681 gacctcaact cttgtatcgg ttgcggcgcc tgcgtggttg cttgtaatgc cgaaaacaat
     7741 attcctgttg taggtaaaaa agaagttatc cgtgcacacg aaatgcattg gatgcgcatc
     7801 gaccgctact acggtggcga tccggaaaat ccggaagtga tctaccagcc tatgctgtgt
     7861 cagcactgcg aaaatgctcc ttgcgaaaac gtttgtcccg tgggcgctac caaccacagc
     7921 tccgaagggt ttaaccaaat ggcttataac cgttgtatcg gaacacgtta ttgcgcaaac
     7981 aactgtccgt ataaagtacg tcgcttcaac tggttcgatt acggttctgc cgatactttc
     8041 ggaacaatga acgatccgag cggaacagaa aacctcggat tgtttgaaga cctcactcgt
     8101 ctggtattga atcctgatgt aacagttcgc tcacgcggtg ttatcgaaaa atgctcattc
     8161 tgcgtgcaac gtattcagga tgcgaaactt accgctaaaa aagaaggtcg cgaattggcc
     8221 gacggtgaaa tccgcactgc atgtcaaagc ggttgccctg ccgatgctat cgtattcggt
     8281 aacaactacg atgagaagag tgcaattaca acagaaacta tggatgaaag aaccttcggt
     8341 attctcgaag aattccactg gttaccatct gtaacatata aaactaaggt aaggaacaaa
     8401 gacaaagccg acagtcacgg tactgaagcg ctcgatatca tggatttgtt caattataaa
     8461 taattcaacc acttaagcag ttatgttcgt atcaccttta agggaaccgc tgatccaagg
     8521 tcataagacc tacaaacaga tatctgatga tatttgtgcg ccggtagaag ccaagccgag
     8581 caaggcctgg tttattgcca ccaccttgtc gggactcctc gccatcaatg gctttgtgat
     8641 gatcggcctt accattactt acggtattgg tatgtgggga ttaaacaaaa ccgttggttg
     8701 ggcctgggat atcaccaact tcgtttggtg gatcggtatc ggtcacgccg gaaccctgat
     8761 ctcagccatc ctcctgttgt tcagacaaaa atggcgcacg ggagtgaacc gctcagcaga
     8821 ggcgatgact atcttcgcgg taatctgcgc gggcttgttc cctatcattc acatgggacg
     8881 tgtatggctc gctttcttca tcttccctta tccaaataca cgcggtccgg tatgggtgaa
     8941 ctttaactca ccattgcttt gggacgtatt cgcgatttcg acatacttta cagtatctct
     9001 ggtattctgg tataccggtt tgattccgga tcttgctttg atccgcgacc gtgcgaaatc
     9061 ggcattcacc aaaaagatgt ataacatcgc gtgcttcggg tggaaaggaa cagctaaaaa
     9121 ctggcagcgt ttcgaatcga tttcactggt tctcgccggt ttatcgactc ctctcgtatt
     9181 gtcggtgcac accatcgtat ccttcgactt cgccacatca gtaattccgg gatggcacac
     9241 taccatcttc cctccatact tcgttgccgg tgcgatcttc tccggatttg cgatggtatt
     9301 gacgctgatg ttggtggtaa gaaaagtatt taatcttcag gaatatatca ctctcgccca
     9361 tatcgaggcg atgaataaaa ttatcatcct cacgggatct attgtgggtg ttgcctatat
     9421 caccgagtta tttactgcct ggtatagcgg taacccatac gaacgttatg cattcctcaa
     9481 ccgtgcaatg ggtccttatt ggtggagcta ttggggtatg atgacatgta acgttgtatc
     9541 accacaatta ttctggttcc gcaaattacg tcgcagtgta gtattcacat tcttcatgtc
     9601 gatcttcatc aacgtgggta tgtggttcga gcgtttcgtg atcatcgtta cgtctacaca
     9661 ccgcgatttt attccttcta actggacaat gtattatcct acttatgtag agatgttcat
     9721 ttatatcggc acgatcggat tctttatgac actgttcctc atcttctcta aagtagcacc
     9781 ggtgatagca gtagcagagg tgaaatcgat cctgaaaaca gcgggcgatc aatatacagg
     9841 ccctgatgcc attcataaac acgaagaaga taatcaccat taatccaata acccagataa
     9901 ataaaggtta gcaaatggcc actaaaaaaa taatgtacgg tttatatgat gatgaggtag
     9961 tactcatcaa agccgtaaaa aaagtgcgag cagaaggatt gaaaatacac gattgctata
    10021 caccatttcc ggtgcacgga cttgatgatg caatgggctt gcgtatgaca aaattgcaca
    10081 gcgccggttt tattttcggt gctacaggta cgctgacggc tttgtcgttc atcctttgga
    10141 taactaccaa taattaccct atcaattacg gtggtaaacc atacttgtcg cttccatcat
    10201 ggattcctat tactttcgag cttacagtat tgtttgcatc agtaggtatg ttccttacat
    10261 acctctatct gaacagactt gctccgggca tgaaagcaga gatcgttgat gaacgcacta
    10321 cctgccacat gtttgcaatg actttcgaaa ttgacgacca tactaccgat gccaagaaaa
    10381 ctgaaatcag aaaagtattg agcgatagcg gtgctgtgga aatccatgaa aaagaacttt
    10441 aatacagaaa atccatacca atggtgaaaa gaaacataat gatcgctctt gttgctgctg
    10501 tgatgattcc ggcagtgctc acttcttgta aaaaagcaag cggtgattat ccgggcgact
    10561 cctatacttg ggatatgagt tattctcgtg cttacgaacc atataccctg aaccctgaat
    10621 tgcgtgatag catgaccgct atgactcctg ttgcaggtac tgttccttat gtaggtaatg
    10681 cagttgccgg tgtgaaagcc gacagcttga aaatggcact caacctgcca tataccattc
    10741 caaatacacc ggaaggttat ttgcgcgcag ctaccgaaaa tcacaaccct tttagtatta
    10801 atgacgaaaa ggttgtagca cagggacaac atttctttaa tatctattgc gctgtttgtc
    10861 acggtgttga aggaaacgga aaaggattta ttgttacaga aggaaaatat acagccatgc
    10921 caccttcata tttcgatccg atgtatatgg cgatgacaga aggcacgatg ttccattctg
    10981 taacgtatgg acgtaacgca atgcaatcat atgcatatgc tttatcgaaa gaagagcgct
    11041 ggaaagtgat tacttatgtg aagaaaatgc aagcagatta tgccgctcaa aatgctgcaa
    11101 ctgctgctac aacaaccggc actaattaac agattacaac cactaacgta aaaaaaggaa
    11161 atcagaatag catgaacggc tcaaataaat ttgaatacac ggccaagctg aagaatcgct
    11221 tcttaggcat gctcgtgatc ggagtagtac tcgtcgttgt gggcatgtta accggtgcct
    11281 ccatggacag attctggtcg aattatctgc aaaacacttt attcttttta ggaatatcgg
    11341 ttttagcagt attcttcctc gctgctcacc agatcggtat ggccggctgg catatcactg
    11401 tgaaaagaat acctgaagca atgagccagt tctcgaaagt gggtgctgta ttaatgctcg
    11461 tggtagtttt cggagtgatc ttcgattatc atcatttata ccaccattgg acaaatacct
    11521 ttattcagga aaaagaagtt tctgttgaag cattgaaaga ttacgaagca gaacttgccg
    11581 aagaaggtca tagcactgag catgccgttg attacaatcg cacctggcag gattttcaat
    11641 atcatcaggt agctcctgct cctgaacatg ttgatgcagc aaaaccggaa cctgaagtgt
    11701 tggaaaatgg aatggttgta aatccatatt acgatcgtct gatcgatcat aaatctgctt
    11761 atctgaataa aacattctgg agtatacgtt ctatcagcta tttgtttttg tgggtgttaa
    11821 tggcaatgat tattcgccgt tattcattgc gcgaagacct tgataattca gatacttttt
    11881 atcagaaaac gcgcaaagct gctgccgtgt tcctcatcat ttgggctgtg agcagttcta
    11941 caatgacatg ggactgggtg atggcactcg atccgcattg gtatagcacc ttgtttggtt
    12001 ggtataactt catctctctt tttgttgcaa gtatttgtac taccattctt attcttattt
    12061 atctcaagcg caatggttac ctgacacaat tgaatgagaa ccacttgcat gatttaggta
    12121 aatatgcatt cggcttcagc gtgttctgga catacttgtg gttcagccaa ttcatgttat
    12181 actggtatgg caacattcct gaagaaacac gttggtgtct cgaccgcgca cgcacagact
    12241 acaaatggtt gttctatgcg aacctcttac tcaatttcct tttcccattc ctgacattaa
    12301 tgcgtcgtga tgcgaaacgt aatatgaatg tgctgatgat tgtagcaatc ggactcatca
    12361 ttacccactg gttggatttt tacatgatga ttacacctgc aactttaggc acagaagtgc
    12421 actttggaat tctggagatc ggaatgttta tcgcttatgc cggcctcttc ttgtttgtgg
    12481 tattcggtga gcttgcgaaa gcatcactca ttccgcaaaa tcacccgatg tataaggaaa
    12541 gtcttgacca tcatatctaa aatcaaatta tcacaatggc taccggttgg ttgatattta
    12601 ttgcaatttt aatgatcgtt gtggtcttgt tccaggtaac aagaacactc gacctagttg
    12661 gacaacttcg cggaggtgat actaccaccg taagtatgac caaatttcat gcttcactcg
    12721 gaatgatatt tatggtgttg ggtatgatcg gatttttctg gtcattcgga catttccatg
    12781 attatcttgt agatcaccac tcatctgata acggtcttat tattcatagg atgttcgtaa
    12841 tcacattgtg ggtgacagga attgtattcg tgatcacgaa tgtgttgtta tttattttcg
    12901 tttatcaata tcgccaccgt cccggtcatc aggctacgca ctttgcgcat agcaataagc
    12961 ttgagtttat ctggaccgtt gtgcctacaa ttgttttgac aggacttgtg gtttttggtt
    13021 tgcaagcttg gaccgatatc atgggtgatg ctgaggacga tgctattgtg tttgaagtaa
    13081 ccggtcagca attcttttgg agtgcacgct atcccggacc tgatggaaaa ttaggacctc
    13141 gcgataatga tttgatctgt gcagataatc cgcttggcat tgttacccgc gaatggattg
    13201 accatcgtcg tgtgacactc atcggtagca ctaaacttgg tgtgaaaggt gaaattgaaa
    13261 aattgcaaga tcgcgaaaaa gagatcaatg ctattattgc ttcttatgag caacgtatcg
    13321 caacatcacc aaataaatat aaagttgccg atgcgaaaga cagtctcgaa atcgtgaaaa
    13381 aagaattagg aaaaattccg ggcaagatcg aaatacgcga aaaggctctt accaggatta
    13441 ataaaaaata tacggaagaa tatatcaatc aacatgcagc ggaatttact tggggatatg
    13501 atgatccaat gccaagtgaa gtgcatgtgc cggtcgacag acaagcattt gtgaaaatca
    13561 cggctcttga tgtattgcac aacttctata ttccgcacat ggatgtgaag atggatgcag
    13621 ttccgggtat gccaacatca ttcaaattca agccactcat tacaactgcc gacaagcgaa
    13681 ttgaaatgtc tgaaaatcca ttgtggcagg taacaaaaga aggcgaaaaa gagcctcgct
    13741 ggaaaaattt caattatgag atagcatgtg ccgaattatg tggttccggt cacagcgcaa
    13801 tgaaatatac acttgttgta gaaacacaag aagattacga caaatggata tccgcacaac
    13861 aaagtaaatg gagtcaggta gcagcttcat tgaaactgga aaatggtttt tcaaaatttg
    13921 ctccgaaact tgaagctcct gctgcagaaa cacctgcagc acctgctgat acaacaatga
    13981 ttacaaaaaa ataattgaca gaaaaataaa ttggtatgtc agaacatcac gcagtagcgc
    14041 atcacaccga agcccatgca cacggtcatg atcatcacgg tcacgacgga catcatgagc
    14101 tgggttttat tcgcaagtac attttcagcg aagaccataa aactatcgcc aaacaatttt
    14161 tggtgacagg tattttctgg gccataatcg gtgccttgct ttcggtattg ttccgtattc
    14221 agctcggata tccgaatgct gatcttacct ggatgaaacc attcctcgga aaatggatca
    14281 ccgaagaagg acagatcgct cctgaatttt ataacgcgtt gatcaccatg cacggtacta
    14341 ttctggtgtt cttcgtactt actgcaggat tgagcggaac attttcaaac ttcctcattc
    14401 cattgatggt aggtgcgcgc gatatggctt ccggatttat caatatgctt tcatattggt
    14461 tcttctttat ctccggtgct gtgatgtttg tatcattctt cctggctacc ggtcctgcat
    14521 caggtggttg gacagcttat ccgccgttaa atgccttaca tgatgcctct tcaggtggtg
    14581 tgttaggggt agattattgg ttgattagta tggcattatt tattgtatct gcccttctcg
    14641 gaggtttgaa ctatatcact acagtaatca acttgcgcac aaaaggtatg aagatgttcc
    14701 gtcttccatt gcctatctgg gccttcttcc ttactgcggt aatcggtgta ttgtcattcc
    14761 ctgtgttatt gtcggcagca atcttgttgt tattcgacag gaaccttgga acaagcttct
    14821 atcttgatgc tattaacctg gcaggtgaac cattggcgaa ttccggtggt agcccaatct
    14881 tatatcagca cttattctgg ttcctcggac accctgaggt atatatcatt atcctccctg
    14941 cgatgggaat tgtttcggaa gtgctatcag tacattcacg taaaccgatt ttcggatata
    15001 cagcgatgat ttattcattg ttggcaatta ctgtattgtc attcatcgtg tgggcacacc
    15061 acatgttcat gacaggtatg aatccattca taggttcaat cttcgtgatc tttactctga
    15121 tcattgctgt tccatcggcg atcaaagtat ttaactggct tacaacgttg tggaagggcg
    15181 atattcgact taatccgcaa atgctctttg cgataggatt tgtttcgtta tttatttccg
    15241 gtggtctgac aggtatcttc ttaggtaact cttccatcga tattcgtttg cacgatacct
    15301 atttcgtagt tgcccacttc cacttagtga tgggtgtggc tgcattcttt ggaatgtttg
    15361 ccggtgtata tcactggttc ccgaaaatgt ttggtagata tttgaacccg acattgggat
    15421 atatccactt ctggggaacg atctcagccg catatttaat cttctaccca atgcacttcg
    15481 aaatttcatt gcctcgtcgt tattatacac actcaggctt tgagacattc agcgatttcg
    15541 cacatttgaa tgcattcatt tcaacggttg ccatcatttc atttgcattg caattgttgt
    15601 ttgtattcaa tttcttctat agcatctggt atggtcgcaa attgcgtgaa tcaaatccat
    15661 acggcgcaaa tacattggaa tggactacac cattgcgtcc gggacacggt aactgggaag
    15721 gagatattcc tgaggttcac cgttggcctt atgactatag caagcatggc agagatttta
    15781 ttcctcaaac agaaccattg tcgcaatatg aaatagaaca tggcgaacat ggcgatgaag
    15841 gtgataggga tatcattaaa aaagaagaag acgaaaatca caaaggataa tttataaatg
    15901 cgtaaagatt cgtccatagc aggatccggc ataggcaaaa tttttgctgc gcgactgaaa
    15961 gactaccaca tgttggtgaa gttcagattg agcttcatcg tggtattcag ctcgggtatc
    16021 ggatatttat tctgcggtgg cgaacaatcg caattctttc tattcttgtt aggcggattt
    16081 ctcattacgg cagcatcaaa tgccatcaac cagatcatag aatcagatac tgatcagttg
    16141 atgacacgta ctgccgaccg accactggct gcaggaagaa tggcacatac cgaagccatc
    16201 atagcagcag gtgtaatggc ggtatcggga atcgctttat tgtggtggca gtttaatagt
    16261 ctcagcgcct tgcttggtgc actttcactc atcagctatg catttattta tacgccgatg
    16321 aagaaggttt ctgcaattgc agtatttatt ggggcctttc cgggagcaat gccaccactg
    16381 attggcggtg ctgctgcaaa tggagagatt tccaatatcg cgatattatt attttgtgtg
    16441 cagtttgtat ggcagttccc gcatttttgg agcattgcat gggtggcatg tgaagattat
    16501 gcgaaagcag gattccattt gttgcctaca gctagcggaa gatcaaaagc atcagcagtg
    16561 catattttgt tgtatgccgc actcttgatt cctgtttcct tgttgaccta tttcctgggt
    16621 tataccggaa cagtatctgc agtaatagta gctgtaacag gattgctttt ctttttgcag
    16681 gcagtgcatt tatttcgcac agcagacact aaagcagcac gcagtttaat gtttggatca
    16741 tttttgtatt taccgatagt attaattgca ttattggttg atcagatata aataaacatg
    16801 gcagtatcaa tacataaacg cttcatcgtt catccatata aattcaatat gtggattgct
    16861 atcgtttcga tggtgatgat ttttgcagcg tttaccagtg cctatgtggt gaaaaaaggt
    16921 gatacaaaaa cctgggcgga tatacaattg ccgcaaattt tcaccttgtc aactattgta
    16981 attgttatca gcagtttatt gatgcacgct gcttatgttt cctttaaaaa aggagcattg
    17041 gaatggtatc gcggatttat gacgcttact tttttggctg gtgccgcatt tttaagtatg
    17101 caaattcaag gttggtatac tcttgtagca cagggcgttt atcttaccga taatgttgca
    17161 ggaagtttta tttatgtaat ttcaggagcg cattttttgc atgtggcagg aggggtgatc
    17221 gcactactgg tgttcagtat tcgttcattc acccgctatc aggcatcgaa tatagaagca
    17281 atgaaggata ctaccattaa tgtcgaagta atggccacct attggcattt cgtagatatt
    17341 ttatgggtat accttttctt tttctttatg ttaaaccgat aaaaaccaga tattttggaa
    17401 gcagtcgcaa caacaaatgc aaaagacctt tggtcaggtg gtgactcccc cttcaaggtg
    17461 agttatggca agatcatgat gtggtatttc ctcatcagcg atactttcac ctttgccggt
    17521 ctgttgatct cttacggagc gatcagaact agtcagccta aacatgatgc cgctaacccg
    17581 ctatcatggc ctgatccgaa cgaagtgttt aacgcaggtc ccggatttca caatgcaccg
    17641 ctgatcttcg ttactttcat gacgttcaca ctgatcttaa gttcagtaac gatggtacgt
    17701 gccgtgcagg aaggtcatca gatgaatagg agtggagtgt tgaaatggat ggcatttacc
    17761 attcttggtg gtttggtatt cgttggcagc caggcatggg aatggtcgca tcttattgaa
    17821 gaaggaatga caccacatgg caacccattc ggtgcatctt catttggtgc cttgttcttc
    17881 ctgatcaccg gatttcacgg tttccacgta agtgttggtg tacttcttaa ctcatgggca
    17941 tttatttcta cataccgcgg agtatttgat cgccgaggcc actatgaaat gatcgaaaaa
    18001 ctcggtctct actggcactt tgtggatctg gtatgggttt atgtgttctt agcattctat
    18061 ctattctaaa caaacgatat tcagcaatat gtctacttta actcaagaag aatcaagaaa
    18121 acgcgtatgg cgagtgtgtg gaattttggc tgtagttact gcagttgaaa tcgccgcagc
    18181 attgatccat tacaactaca tgcacgactc tcctaagtcg ttcctgaata ttttgttcgt
    18241 tgtactcagc gctgccaaag cctattatat catgagcgaa ttcatgcacc ttaagtatga
    18301 attaaaacct atggtaatca gtatcctggc accgttcctc ttcctcgttt ggggtattat
    18361 cgcattcctc tgggaaggac actactggct gagcgaaaag gaactttggc atatactgaa
    18421 ataatatgtc ttcaaccaga gggcgtatat ggaccatcgt gatgctggtc atcattctgg
    18481 tgttgccaag tatcgcctat ttaatgttac gtgaaggtct gcatgtcagg gaatcagccc
    18541 ctgcaagtga tcatttgatc aaggagaatc ttcaatccat tcccacctat tccggggtgt
    18601 cgtttagagg cgataccatt tctaaaagta ggatgaaagg gaaggtttgt atactgaatt
    18661 ttactactca tgcctgcgaa aaatcgatgg atgataacat gcgcaagctg tttgaaattc
    18721 aggaagatta ttacggaaaa acaatttctt tccgcattat cactaatacc ttagatcccg
    18781 ataccgacgg cctggataat atgcgtctga tgagtgagcg ctatgcagga cgcgaaatct
    18841 ggcattttat caccgatccc gatagctcag gtgttaaatt atatgattgg tgcaaaaaaa
    18901 atgcaacaga tagcacctta aatgaaactg atacgcagtg tccgcagtta gtatacctgg
    18961 tcgatggcaa tggcaaactc agaggtgtat acaatattct cgaagaacag caattccacg
    19021 acttgtataa cgacattttg tacttggtga atcaaatgga actgcatgag caaaagaaat
    19081 aatctcatca tttttggagt cagcggcgta gcatttattc tattcgtcat ctttgtttac
    19141 ctgccatatg ctaaaaagca ggataatctc aataaggtaa aagaaagtta tcagcatagc
    19201 tcatatctga aagatcctgt ggggcaattg ccggtattta gttgtatcga tcagaatggg
    19261 aaggtattta ccaacgacag tctgaaggga gatgtggtgg ttgcaaattt cttttataca
    19321 acgtgtgaag gctattgtcc gacaactacc aaacacctgg ctacggtgca atcatcattg
    19381 aataaaaata ttccttttca cattatatca ttcagtctta atcctgcaaa tgataccgta
    19441 gcagcattgc gcaaatatgc tgatatgtat aaggctgatg ataatgtatg gcgttttctt
    19501 cacggcgatc aggaagaaat ttatcagtta ggtgaacaag gcctgatgac gattgttaag
    19561 gacacagcgg gcaattttga atctcattcc gacaggtttg tattgatgga taagatgggt
    19621 aaaatcagag gtttttatcg gggaacggat tcacttgaat tgcaggcact gattcaggat
    19681 atcaattatt tggtatttaa aggagagact aatgaatgaa cgtacaataa ataggtggat
    19741 tgtagccatt tcaatattga ttccggtagc ggttaccatt cttttttata tgcctgcggt
    19801 gaagttgaat atcgatactt ccatattgcc ggcctttcat gcaatgctca attccggagt
    19861 agcaatttta cttttacttg gattgtactt cattcgcacc ggaaaaagag gggcacacag
    19921 aacgtcaatg cttggtgcat tttcattgtc ggcattattt ctgatttcat acgtgctcta
    19981 tcatctcaca tcagactcca ccttgtttgg cgatttagat cataatggca ttgtttcaga
    20041 tgccgaaaag gctgaagccg gacccatgcg tggggtatat ctggtgattt tattgtcgca
    20101 catattcctg gcagcgctga tcatgccatt tatcctggtt actttgtcaa gggcgctgac
    20161 agccaggttc gataaacatc gaaaaatcgc tcgaatcacc tggccccttt ggctttatgt
    20221 ggcgattacg ggagtggtag tatattttat gattgcgcct tattatccgt aaccaaaata
    20281 gctgtaaatt tgccttatga agtatcgctt gttgattatg ttagtgcttg tggtgctgtt
    20341 gtcagcgctg cataccgacc ttcaagctca gtgtgccatg tgtaaggcta ctgcggaaga
    20401 aggattgcgc gaagggaata cccaaagtgc caatctgaat gcaggtatta tgtatctgtt
    20461 ggcaatgcct tatctggcct tcggagtaat tggattttta tggtatcgca attataagcg
    20521 taaaaaagca caggaagctg ccggtctctg atcagagatc tcccaattgt ggtcttatta
    20581 gaatttcttc tacaactgtc ctgtcggaaa gttggtaagc ctgtaatatt aaggtagcaa
    20641 tgtcatcggc aggaataaat cttgattccg gcaatggggt gccatcccag gaaggtgtta
    20701 aaactgcacc cggaagcaca gcggtaacgc gaatattatc ggtttttaat tcgtggcgaa
    20761 gattcttagt aaaaccaagt aaggcgaatt ttgaaatagc atagctacca ccgccatcat
    20821 aggaattgat actggcaaca gagcagatat taaaaatgta tccctttttt gcagtgcgca
    20881 taacagggac gatttgtctt gtaagatgat atgcactata tacatttgtt tcgatgagaa
    20941 attcaagcgt gccctcggct tcttcggtga tattacccgg tagaaatact ccggcgttgt
    21001 tgatgagcac atcaatagaa ccgtatgctt taaggcaatt ttcgccaaac gaatgcaatt
    21061 gcgctctgtc tcgcacatcg caggaaattg ccaaatgttt acctgtcgga ttggcatcgt
    21121 gtaattgtgc tgacatttca ttgagggctg ctttatcgcg tgcacaaatg gcaacgttaa
    21181 atccattttt agcaaataat aatgcacagg ctttgccaat accctttgtt ccaccggtta
    21241 tgatacaatt catctttgcg aaatagttac atttacaaag taaaacaatt tgacccaatg
    21301 cgtagtgtgt ttgttgatgt gatcttgccg tttgcactgg agaagaatta tacctatgcg
    21361 gttcccgacg agtttgtagg ggtgctttcg cccggcatgc gcgtggaagt gccgtttcgc
    21421 aacaaaacgt atacgggaat agttgctaag gtgcatgata tcagtccgca aggatattcc
    21481 cccaaacccg tgctctcatt gcccgatcat ttgcaggtag taaataaaac gcaacttgat
    21541 ctgtggacat ggatatcgaa atattatatg tgttcggttg gcgatgtgat gaatgcagca
    21601 ttgccggcac catataaatt gagtagtgaa acagttgttg tctacaatcc gcaacatggc
    21661 attgatctgc ttacattgga cgaaaaagaa tatctgatag ctgaggcatt gacattacaa
    21721 caggaattgc gacttattga tgtgcaacag atattgcgac agaaaactgt tcagccgata
    21781 attaaagggc taattgcgaa agggattgca tttattaaag aagaattaaa ggaatcatat
    21841 actgtaaaga ctgaaaaata tatcattctg acatcgcaat atagatcaga agatgccttg
    21901 caacaattat ttgaatcact gcaaaaaagt gaaaagcaaa caaatgtttt aatgatttat
    21961 taccagcttt ctgcaaaaga gggaaaggtg aagaaatcag ttttactcag gcgatcgggt
    22021 gttacggcgg cggtgttgaa gaccatgatt aagaatgaca tttttgagga ggtagaagag
    22081 caggtgagtc gtttgcaagc ctatggaaat gcagatatgc atgacgcgca cttgaatgca
    22141 gatcagcaga aagcattgtc ggagatagag gagcaatttg aggaaaagca aactgttctc
    22201 ttacatggag tgacaggcag tggtaaaacc gaagtgtata ttgaattaat gctcaagaca
    22261 attgctgcag gaaaacaagt attgtattta ttacccgaaa ttgcactcac ggcacaaatt
    22321 gtgacgcgat tgaaaaagcg attcgggaat ttaattggtg tttatcattc aaaattcaat
    22381 cagaatgaac ggattgaaat atggcagcat gtattgaaca atgaattcaa agtaatattg
    22441 ggtgcacgct ctgctttgtt tttaccgttt aatgaaatcg gattaataat tgttgacgaa
    22501 gaacatgatt attcatataa acaacatgag cctagtccgc gctatcatgc cagagatact
    22561 gctatttatt tagggcattt acatcaggca aaagtgttat tgggaacagc aacaccttcc
    22621 gtggaaacat attatcaggc gaaatcaggg aaattcgggt tggtggaaat gtttcgacgt
    22681 catggcgata tgcccatgcc aacgatacag atcgtggata tgaaggcaga ggagaaacaa
    22741 aaaaccgtgc aaggacattt cagcagtgtg ttggttggaa atatcaagca tgcgctgcag
    22801 ttgaaggagc aggttatttt attccagaac aggagagggt atgcgccatt tatgatttgt
    22861 cagtcatgtg gctggacacc aaagtgtacg caatgcgatg tgaatctggt gtatcataaa
    22921 tatgccaatg aattgcgctg tcattactgt aaccacgttg aaaatacgta ttcagtctgc
    22981 cctgcgtgta atagttcgca attaattatt aaaggctttg gcacagagcg catcgatgat
    23041 gatttacagt atacatttcc tgatgcgcga actgcacgac ttgatttgga tgccataaaa
    23101 accaagcatg gtcatgaaaa attggtaaga tcttttgaag agggtgagat agacatatta
    23161 accggcactc agatggtgac caaaggcttg gatttcgatc atgtgaattt ggttggcata
    23221 ttgagtgcgg atcaaattat caatttttcg gacttcagag cagccgaaag ggcatttcag
    23281 atgatcacgc aagtgagtgg aagggcaggg cggaagcata aaaagggttt agtattgatt
    23341 caggcgataa aagttgatca tccggtatta caatatgtga ttaccaatga ttttcatgga
    23401 tttt
//
LOCUS       DAOQOU010000035        23288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1047_length_23288_cov_5.826195, whole genome shotgun sequence.
ACCESSION   DAOQOU010000035 DAOQOU010000000
VERSION     DAOQOU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23288
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1047_length_23288_cov_5.826195"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLM90_04925"
     CDS             complement(<1..322)
                     /locus_tag="PLM90_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88743.1"
                     /translation="MRRVPGMKIISTNRLRWFWLLAVLFSTNIAFGQGTQVQFGQNRV
                     QYKDFDWQFYEGEHFQVYFSAGGLNLGKFVAQIAEADLIQIEDLLDYKLNSKPEIIVY
                     NTISD"
     gene            395..1045
                     /locus_tag="PLM90_04930"
     CDS             395..1045
                     /locus_tag="PLM90_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ88744.1"
                     /translation="MASIISFTFNAFYENTYVIYDRTGACVIVDPGCYDRAEEDDLVD
                     AISKKGLKPALLLNTHCHVDHVLGNKFVSDTYQIPLVMHRGELPILQNVVNYAPSMGL
                     RYRPSPDPERFVVDGDTITFGETTLDVLYTPGHSPASVCFYCKADNFIVSGDVLFHDS
                     IGRTDLPGGSMPTLMHSIRTKLMNLPDETKVYAGHMQSTTIGRERLHNPFLTGTYA"
     gene            complement(1068..1571)
                     /locus_tag="PLM90_04935"
     CDS             complement(1068..1571)
                     /locus_tag="PLM90_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88745.1"
                     /translation="MNNKCTIIIIAVMLIASGCASYPKGINPVGKNKTDPYGATMKVS
                     PKSTKTIIGELIAVNGDSLYLLGANNQIMRYHIKDIRTYVVYYGTPEKTLGWTYALLP
                     AISISHGFWMILSIPINLIFAGGITNDVKQDMIYTNRELPIDKLSIMARFPQGLPPSV
                     SLNEITR"
     gene            complement(1564..3177)
                     /locus_tag="PLM90_04940"
     CDS             complement(1564..3177)
                     /locus_tag="PLM90_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88746.1"
                     /translation="MLHKLHIRRLFHITLLLVVIAGISSCGTTQFRKDYYNVNTLLHD
                     STQFATGTPWLKAHMKNGNIVIFYNTWEVDSAQTYISGFGAQYDFNRQLITQDHLSIP
                     IDDIALFETNNPVQDNKGRISAITIMTGLDILFGVVCITVPKACWGSCPTFYIDSTAT
                     THYADAEGFSNAILPSMQYGDIDALPGSVLPGSTFRITLRNEALETHCIDKVTLYAIS
                     TPTNKTAFQSVDNKFYLGDKNFICSKAYDENGEITALLEKADTQERFSTTDAHNLKSK
                     EAIYLNFPSAQANDSIGLLLRFRQTLLPTYCYYNAVSYMGDFVGGINTLLETDEKIRN
                     KFGHDGFLGALGGIDVHVWNESENDWKYEGAFNETGPIAINTQILPLSAFDSKDSIKI
                     KLVLNKGLWRIDQAALVTDLHRTQAEAISPHVINAAGGHEDAFSDYLSDSTNYLITYP
                     GEHFYLDFPMPEQSDHYTAFLYSEGYYLEWMRGDWLADKNILKLRYMLNFPNLYLKTE
                     ASVFKEHEAAMEQIFWNSRISQPIFSSNE"
     gene            3250..3384
                     /locus_tag="PLM90_04945"
     CDS             3250..3384
                     /locus_tag="PLM90_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88747.1"
                     /translation="MKHLTQGKVSLKGGSESITYRNRLSGDDQPVCFDGCNFFGRYDK
                     "
     gene            complement(3630..5498)
                     /gene="htpG"
                     /locus_tag="PLM90_04950"
     CDS             complement(3630..5498)
                     /gene="htpG"
                     /locus_tag="PLM90_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HMZ88748.1"
                     /translation="MSIETGNIAVQTENIFPIIKKFLYSDHEIFLRELVANAVDATQK
                     LRKLSSMGEIGGELGDTNIRISIDKEAGTLTISDRGIGMSGDEVKRYINQVALSSASE
                     FLDKYKGLEDGKSIIGHFGLGFYSAFMVADQVALVTRSAREGHDAVRWTCDGSPQFTL
                     EVVEKETRGTDVILYINEENKEFLEDNRIQTLLDKYCKFLPVPIQFGESVTKEKDEAG
                     NETEVTAPRIINNPNPAWTKKPAELTDEDYTNFYRELYPYAEVPMFWIHLNVDYPFNL
                     TGILYFPRIKKSYEIQKNKIHLYSNQVFVTDEVNAVVPEWLTLLHGVIDSPDIPLNVS
                     RSYLQSDQNVKKINSYITKKVAEKLEELFKKDRAAFEEKWENINVIIKYGMLSDEKFF
                     ERGEKFCLLQNTEKKFFTLDEYKAHIAPAQEDKNKQVIALYTNNVTDHHVYVDAAINK
                     GYDVLEIDNVIDNHFISFIEQKMTGVQFKRVDADTIDQLIDKDEKIESVLSDTEQTSV
                     KELFEQVVDANAGTVVLTPMSPNDQPVTITKPEFMRRMKEMSHLNGMDFAASMPDQFN
                     LVINTNHPVVNKMLQLDETHRLNTARQLHDLALLSQGMLKGTALTEFVKRSVEQMQ"
     gene            5616..7439
                     /locus_tag="PLM90_04955"
     CDS             5616..7439
                     /locus_tag="PLM90_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ88749.1"
                     /translation="MPTSGRLNRKQIEAEISGLLHTSWDLVSKRNSEIAELSSKALAL
                     AEQINDNNLKGLCILEGALYQCLVKSDYHLSIKMSDEGYAMMKGEFKKRYAPYYHLNV
                     GRNYHFMGDHVSAQHNYLECVKCIESMSEQNQFELKWLAHAYYNLYILFNFSGAEFAQ
                     AEYLQKALTIYKQIGDVGGEGNCYNSYAVYYFRLNNYTQAYENLQRAYALAEQEQATS
                     LLSIYSANIGLVKVKMGEVETGFTFFDKAQHYDLELDSPYHHAHTQNQMGEAFIILER
                     YEEAIERFAKALQYFESIGVKRSMSNTYEQMAKAYAGLGNHERAYHYQQAYAQSLKDV
                     FSDEKTFAVAKARNDFELEMRDQEARKLRLKNKQIEQYARQLEMSNDELRQFAHVASH
                     DLREPLRMVSSYVQLLQRSMDIPLTGDQQEFMRYILQGTHTMQNLISDLLALAAISNV
                     RNSELVDLNIIVQQVKSNLQPVIEERNAVIKSTLLPAVIADETHMLQLFLNLISNGIK
                     YNTSTQPLIEISFATSGRYYQFSVADNGIGIPAEYKEKIFLIFQRLHSKEEYSGTGIG
                     LAICKKIVDQAGGTIKVSSRKGGGSIFTFTLPVIEHQPESV"
     gene            7544..8008
                     /locus_tag="PLM90_04960"
     CDS             7544..8008
                     /locus_tag="PLM90_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HMZ88750.1"
                     /translation="MRLLAGIAFCLMCASCASTQELQYKGFDDFTVTNILKEPKVQMD
                     LNLYNPNPIGGTIRNMEINIAVDQKTLGSVSLDDKVRIKKQAEFTMPISFTTSVSEMG
                     GILSAGLKSFVGQDEMPLGISGEITIQKFLVFRKTFSFEYKDGIDIHDIIQK"
     gene            complement(8017..8511)
                     /locus_tag="PLM90_04965"
     CDS             complement(8017..8511)
                     /locus_tag="PLM90_04965"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HMZ88751.1"
                     /translation="MAAHWYVAYVRSRSEKKVAERLEKNGITSYLPLIKSVRQWSDRK
                     KIVSIPMFSGYIFVHCLEEQLTNVRMVSGVANFIYREGKPARMPESDISTIKSLLNAE
                     FHTEVHPDSFAIGTQVKINFGPMAGTIGELVAIENEKLFLLRIDVIQQALLIKVPAAY
                     LEKI"
     gene            complement(8514..9548)
                     /locus_tag="PLM90_04970"
     CDS             complement(8514..9548)
                     /locus_tag="PLM90_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ88752.1"
                     /translation="MSSPVIHFAIIGHGHIGKRHAAVIRETPGAIVTAICDVDAQKLA
                     GIDEGVAVFTSITEMLNSNLPIDVVNICSPNGLHAEHALSCLQHNKHVVIEKPMALQV
                     ADCDAVIKLAKDKNKEVFCVMQNRFSPPSVWIKSMLDTHALGKICMVQVNCYWNRDER
                     YYTGDSWHGTRDFDGGTLFTQFSHFIDTMLWLFGDISDIHGIFDDYKHKHLTDFEDSG
                     MVHFRFLNGALGSLQYSTAVYDKNFESTIRIIGEKGTVCLGGQYMNEVTYCHVQDYVM
                     PELAPTAAPNNYGPYIGSAANHHFIIQNVLDVLHREAPIATTAEEGRMVVDVISRIYA
                     LRAQHLTQKN"
     gene            complement(9556..10140)
                     /locus_tag="PLM90_04975"
     CDS             complement(9556..10140)
                     /locus_tag="PLM90_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HMZ88753.1"
                     /translation="MSKQSHFIHSSAVVDDGAIIGKGTKIWHFTHIMPEAIVGEDCVI
                     GQNVFIASRVEIGNRVKIQNNVSLYEGVMCEDDVFLGPSMVFTNVINPRSAVERKNEF
                     QLTRVLKGASIGANATIICGITIGKYAFIGAGAVVTKSVPDYALVTGNPARQKGWMSA
                     HGGKLIFDSKGHAVCDISGINYKLSGGRVQQLNN"
     gene            10189..11670
                     /locus_tag="PLM90_04980"
     CDS             10189..11670
                     /locus_tag="PLM90_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88754.1"
                     /translation="MMRINGRFAMMIMGLGALLTGSCSRDFLGEEALNLAPETYLVTD
                     TIIRFGDDRLESEVTLQWWGDDEDGFVTGYEFTFDEVITDATIWQYTESQDSTLILAP
                     PPGEDSADFVFHIRAIDNHDLADPTPAALTVPVKNSPPAIIIIPGLNTPKKSFPALRY
                     YWESSDPDGAQNLSHVELFFNDTTGTPYILPSTVTSAIFQAQDPTALTLNCEVFANGG
                     TIAEDILMNGLMANAWNKLYVRAVDQSNAKSAFAIADSVFVKQVHSTVLLVNGYSTAT
                     NEAFYMSHLSANGIAVVDTMQIFETVGGVYTQQSADNLTQGRIFAMFDAIIWFSNNAD
                     NSFSLAQKTTESFFNNGGKMLMSVYISSSFDPLSNFLDFTPIASLVNPVDTTLILDLG
                     ASLSAEVADWPDLQGTSIVGVVKPINLQIGATPLYTATLTAKDNVALTFTPWTGVSTV
                     MASKTDGAGQTNFVISTLELNKLDGLLNMDSFFEKVMIDEFGF"
     gene            11654..12265
                     /locus_tag="PLM90_04985"
     CDS             11654..12265
                     /locus_tag="PLM90_04985"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMZ88755.1"
                     /translation="MNSDFEMHVAGGDHRIAIFASGAGTNAENCIRYFRTHASIYVAL
                     IVTNKPTAGVIEVAERYGIPYMIQETWDDEGTELLLSRLAQHRISFILLAGFLSLVPN
                     ELIRKFTGRIINIHPALLPAFGGKGMYGKRVHAAVYAAGVGESGITIHEVDEEYDHGK
                     ILFQAKVAIDGQDTPASIEEKVRALEYAHLPAVVEKLLLTGRV"
     gene            12344..13885
                     /gene="purH"
                     /locus_tag="PLM90_04990"
     CDS             12344..13885
                     /gene="purH"
                     /locus_tag="PLM90_04990"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMZ88756.1"
                     /translation="MQIEHHPVKIKSALISVYYKDKLEPIIHALHRLGVVLYSTGGTR
                     SFIEDLGVPVQAVEDLTLYPSILGGRVKTLHPKVFGGILARRASNEDQQQLTQYHIPE
                     IDLVIVDLYPFEETIRHTTDHSEIIEKIDIGGVSLIRAAAKNYHDVLIIASKDDYSAL
                     QDILDAGGETKLGQRMYFAKKAFNITSSYDSAIFGWMDHGEHLDLRYAPKIGMRYGEN
                     PHQQAAFYGHLSEVVEQIQGKEISYNNLVDIESCIYLMEEFEASKEAGKATFAIIKHT
                     NACGLATADSVESAWDKALAADPVSAFGGVLICNQAIDKAAAGKINELFFEVIIAPDF
                     DAEALALLQSKKNRIILRQRMFKFPVKQFKTVLNGVLMQDRNVKATRADELMKVTDIH
                     PTEAQIQDLLFAEVVVKHLKSNGIALVRNGQLIGCGTGQTSRVDALKQALAKAALFGF
                     DVRGSVMASEAFFPFPDCVQIAHEAGISAVIQPGGSVRDKDSIDYCNTHGLPMVLSGT
                     RHFKH"
     gene            14062..15087
                     /locus_tag="PLM90_04995"
     CDS             14062..15087
                     /locus_tag="PLM90_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMZ88757.1"
                     /translation="MGIFNFLTQEIAIDLGTANTLIIAHDKVMVDEPSIVAIDRKTEK
                     VIAVGKKAMQMHEKTHENIKTIRPLRDGVIADFQAAESMIRGMISMIFPKNRLFKPSL
                     VMVICIPSGITEVEKRAVKDSAEQAGAREAYLIHEPMAAALGIGLDVQEPVGNMIIDI
                     GGGTTEIAVVALSGIVCDQSIRVAGDEFTADIVDYMRRQHNLLIGERTAESIKINVGA
                     ALTELDDAPEDYQVSGRDLMTGIPKQLVVTYSEIAHSLDKSISKIEEAILKALETTPP
                     ELASDIQRNGIYLTGGGALLRGLDKRISQKTKLVVHVADDPLKAVVRGTGIALKNREQ
                     YAALLMS"
     gene            15104..15937
                     /gene="mreC"
                     /locus_tag="PLM90_05000"
     CDS             15104..15937
                     /gene="mreC"
                     /locus_tag="PLM90_05000"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HMZ88758.1"
                     /translation="MRSFFLFIIRHYAIFMFLFLELLSFYFIVTHNSYQQTYFINTAN
                     TLSGNVLNSYGNFQEYFSLREVNDSLMHENAMLREQLLLSQIADTVNHTVSDSTGKPL
                     YSYIPAEVIGNTFTEANNYITLNKGSSDGIQVNMGVITSGGIVGRVVKVSPNFSVVMS
                     VLHTQFVSRVAVKRSNAQGRLTWDGKRPTHIDVIQVSEPGTIKKGDSIITTKVSPMFP
                     PDIMVGTLESIGKDPGSNYYTLDVKLSTNFSSLKYVYVVNEIMSKERNDLENQIQDAG
                     N"
     gene            15924..16448
                     /gene="mreD"
                     /locus_tag="PLM90_05005"
     CDS             15924..16448
                     /gene="mreD"
                     /locus_tag="PLM90_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HMZ88759.1"
                     /translation="MQVINWILRVVGLVFLIMFQVLVLNRLNVSEYVHPYVYPMFIIL
                     LPFDTPRWMLLVVAYLIGFTIDMFNNTPGMHAAACVFIAYFRPQLIKFYTPITGYESV
                     TSPSISELGVIWFTMFTATTILLHHAVYFLIQLFSINDLGFLMIRIGLSTVVSTLLIV
                     ILAFLFAKRKVRAK"
     gene            16573..18480
                     /gene="mrdA"
                     /locus_tag="PLM90_05010"
     CDS             16573..18480
                     /gene="mrdA"
                     /locus_tag="PLM90_05010"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMZ88760.1"
                     /translation="MAVIENLKPRGYVIQAVIAAIFVVFIVRLFYLQVIDDTYKQIAD
                     DRGIRKEQVDPDRGQIFDRNGKLIVYNEPSYKLMVVPSQVKDIDTIRFCEIIGVDKEY
                     FEKKMKEAKNFSRVRESVFLSQISAEDFGRIEEYLYEFKGFYTAITPVRRYTYPAAGH
                     LLGYIAEVDSNDIKKSDGYYRNADMIGKTGVEKSYEKLLRGKKGYHNIVIDRFGRAVG
                     PLNNGSEDVEPVAGKNLTLTIDIELQYYAEQLMVGKRGALVAIEPSTGEILALVSSPT
                     YDPNMLSGSARNKNFAKLALNKENPLNNRALSGYYPPGSTFKPVMAALGMQQGSLTST
                     TGYSCPHGYVMSGHTVDCHNHPYPANVELGIGHSCNAYFCYAFRYFIEDHSNPAEGMR
                     DFKAKLALWGIGTRTGIDLPNERNGNVPSPEDYDKIYGKGRWKASNCVTLGIGQDRLI
                     LTPLQSANSMATIANGGYFYSPHVLKFADGEDSLMNAFTIRKETGISDSIFKYVRHGM
                     AGAVNFGTAKVAHIDSIQVCGKTGTAENPHGKSHSWFSCFAPEVNPKIAVAIIVENGG
                     WGATYAAPIASLVVEKYMNDSISAKRKPLEERMMKSVLILDREVVPPIQNNSNQDTTK
                     QVQAALLPKEQ"
     gene            18477..19904
                     /gene="rodA"
                     /locus_tag="PLM90_05015"
     CDS             18477..19904
                     /gene="rodA"
                     /locus_tag="PLM90_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMZ88761.1"
                     /translation="MREKKSIMENVDQPLLWLYLSLMVIGLLNVFAVSFSDDVKFFDL
                     SLPHGKQFLWMGISLVVGFSILTFESSFFTKFAMVLYGVTIAVLIVTFVVAVDINGAR
                     SWLQVGSFQFQPAEFGKTTTALMLAKFLSIINGQPRSFKNKLLSYLIIGVPMGIIVLQ
                     SDVGSALVYASLVLVVYREGFPVYEVLFVLAIGLCFVFALIFGVDPVAVVLTFIIWLY
                     IVFNSFKLVRKEKIKGIVFLVIFSAGVFFFLIGGEVLGMDTHIAAAVMLIAMIAALIN
                     VRKVRFFQMNIPVVLFAILMVFVMFGVETITTDILEDYQVQRIETLLGMSDDADAEYN
                     VTQSKMTIGSGGVFGKGYLQGTLTQSEQVPEQSTDFIFCTIGEEWGFFGTFIFLVIYL
                     LFILRVIFIAERQRSPFSRVYAYCVASFFFLQIMINVGMTIGLIPVIGIPLPFISYGG
                     SSVLAFSIMIFIMLRLDADRLFILR"
     gene            19991..21472
                     /gene="hutH"
                     /locus_tag="PLM90_05020"
     CDS             19991..21472
                     /gene="hutH"
                     /locus_tag="PLM90_05020"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HMZ88762.1"
                     /translation="METFHIGIQGLTLQQISDIIKNKIRIALSDEAREKIQKCRTYLD
                     NKIASQDKAIYGVNTGFGSLCKVKISDSDLEQLQYNLIRSHACGMGAVIPDEICRILL
                     LLKIQNLAFGRSGVAVETVWRLIDFYNMDLLPVIYEQGSLGASGDLCPLAHMSLPLIG
                     EGELKVNGKIRPTAEVLKEAKLSPLKLQSKEGLALLNGTQFMAAHAVYTLIKADNIIR
                     QSTYIAGVSLEAYDCKSEPFTDQIHQIRGQHGQIAIAKAFMHIMQGSELFFAEKQQVQ
                     DPYSFRCIPQVHGACYDAIQYCKTIVEREINGVTDNPNVFVEEDMILSGGNFHGEPLA
                     LTLDFLAMALSEMGNISERRVYRLLSGARGLPEFLTPDPGLHSGLMIPQYAAASIVSQ
                     NKQLCTPASVDSISSCNEQEDHVSMGANAATKCLRVAINVERILAIELLTAAQAFEFR
                     RPAKTSPALENYIASYRQHVTFNTEDRFLHKDMLTTEKYLQMV"
     gene            21625..22005
                     /locus_tag="PLM90_05025"
     CDS             21625..22005
                     /locus_tag="PLM90_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ88763.1"
                     /translation="MHINQLSGVIVDILIHMHKELGPGLFESVYEEVLASELDKREIG
                     YRRQQVIPIVYDDVVLVENAFKADIIVDNRIIIELKSIDQLSPVHYKQLLTYLRLTGI
                     KLGLLVNFNEVLIKDGIHRVVNNL"
     gene            complement(22037..>23288)
                     /gene="aspS"
                     /locus_tag="PLM90_05030"
     CDS             complement(22037..>23288)
                     /gene="aspS"
                     /locus_tag="PLM90_05030"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMZ88764.1"
                     /translation="KSTPEGARDFLVPSRMQPGLFYALPQSPQILKQLLMVSGMDRYY
                     QIAKCFRDEDLRGDRQPEFTQIDCEMSFVRQEDVWNMFEGMTKHVFKKVKNIDLPDFI
                     RLPYRDAMKYYGSDKPDLRFDCKIQDLNAVVGGSEFGVFNNVLASNGLVAGIVAKGCS
                     EYSRKQIDELTEFVKAPQRGVGGLIYIKFSQDGSVKSSMDKFFTPEQLMAIGESVGAG
                     KGDLLLIVADKEKKTRKVLGDLRLEMAKRENWIDKNAWSVFWVVDMPLFEPDEETGEP
                     IFAHHPFCSPHPDDFQYMDSDPLRVRAQSYDMVMNGNEILSGSIRIHNKELQEKIFKI
                     LGFSEEEKERRFGFMVNAFKYGAPPHGGCAFGFDRWVMLMAGGDTIRDVIPFPKNNNG
                     RDMMMDAPGEVDNDQLRIVGVKLL"
BASE COUNT         6596 a         4671 c         5119 g         6902 t
ORIGIN      
        1 aatctgaaat agtattgtaa actataattt cgggcttact gttgagtttg taatccagca
       61 ggtcttctat ttgaatcaga tcggcttctg ctatttgtgc aacgaatttt cctaggttga
      121 gcccccctgc actgaaatag acctgaaagt gttcgccctc ataaaattgc cagtcgaaat
      181 ccttgtattg aacgcgattt tgcccgaatt ggacctgggt gccttggcca aaagcgatat
      241 tggtactgaa caacactgcc agcagccaaa accatctgag tcgatttgtg gaaatgatct
      301 tcatacccgg aacccttctc accactgcaa aatacatact tttgccgtaa agttcgcccg
      361 cttcattaaa attttacaat ttaacgatag aaacatggcc tctattatct ctttcacctt
      421 caatgccttc tacgaaaata catacgtaat ctatgataga acgggtgcct gtgtgatcgt
      481 ggaccccggt tgttacgacc gtgccgagga ggatgatctg gtagacgcca tttcaaaaaa
      541 aggcctcaaa cccgcgctgc tgctgaatac gcactgccat gtagatcatg ttttgggcaa
      601 taaattcgtt tctgatacct accaaatccc cttggtaatg cacagaggcg agcttccgat
      661 attgcaaaat gtggtgaatt atgccccttc catgggcttg cgctatcgac cttctcccga
      721 tcctgaaaga tttgtggtag acggcgatac catcaccttt ggcgaaacca cccttgatgt
      781 gttgtatacc ccggggcatt ctcctgccag tgtatgtttt tactgtaaag ccgataattt
      841 tatcgtaagt ggtgatgttt tattccatga ctctataggt cgcacagatc ttccaggagg
      901 aagtatgcca acattaatgc atagcatcag aaccaagtta atgaacctgc ccgatgagac
      961 aaaagtatat gccggtcata tgcaatcgac aaccatcggc agagaaagat tacacaatcc
     1021 ttttcttact ggaacctatg cgtgaagtaa aagaaaaaga ccaggtttta tctggttatt
     1081 tcatttagtg agactgaagg tggcaagcct tgcggaaatc ttgccatgat gcttaatttg
     1141 tcgataggca attcacgatt agtgtatatc atgtcttgtt taacatcatt cgtaattcct
     1201 ccggcgaaaa tcaggttgat tggtatagaa agtatcatcc aaaatccatg tgaaatggaa
     1261 attgcaggaa gcaaagcata tgtccaacct aaggtttttt caggggtgcc ataatatacc
     1321 acgtaagttc ggatgtcctt aatatggtaa cgcataattt gattgtttgc accaagcagg
     1381 tataaactat ctccattaac tgcaataagt tcaccaatta tggttttagt ggacttaggg
     1441 ctcaccttca tggttgcacc gtagggatca gttttatttt ttccgacagg attgatgcct
     1501 ttcggatagg aggcgcagcc gcttgcaatg agcattactg caataatgat tattgtgcat
     1561 ttgttattca ttgctgctaa atataggttg tgaaattctt gaattccaaa aaatctgctc
     1621 cattgcggct tcgtgttcct taaatacaga ggcttcggtt ttgagatata gatttgggaa
     1681 attgagcatg taccgaagtt ttaatatgtt tttatctgca agccaatcgc cgcgcatcca
     1741 ttccagatag tatccttctg aatataaaaa ggcagtataa tgatctgatt gttccggcat
     1801 tggaaaatct aaataaaaat gttcaccagg ataagtaatc aaataattgg tgctgtcgga
     1861 aagataatcg gagaaggcgt cttcatgtcc gccggcggca tttattacat ggggtgatat
     1921 tgcctctgct tgtgtacgat gtaaatcggt caccaatgcg gcctgatcta tacgccacaa
     1981 tcctttattt agtaccagtt ttatttttat ggaatccttg ctatcaaaag ctgataatgg
     2041 caatatttgc gtgttaatgg caatgggtcc tgtttcattg aatgcgcctt catatttcca
     2101 atcattttcg gattcattcc acacatgtac atcaatgcct cctaaagcac caaggaaacc
     2161 gtcgtgaccg aatttatttc tgattttttc atcagtttcc aataatgtat tgatgccacc
     2221 aacaaaatca cccatgtagc tcacggcgtt ataataacaa tatgttggca gcaaggtttg
     2281 tctgaaacga agcaataaac ctattgagtc gtttgcttgt gcagaaggga agttgaggta
     2341 aattgcttct ttgcttttta agttatgcgc atctgtagtt gagaaacgtt cctgagtatc
     2401 tgctttttca agcagggcag taatctctcc attttcatca tatgcttttg agcagataaa
     2461 atttttatca cctaaataaa atttattgtc gacactttga aatgcagttt tatttgttgg
     2521 tgtagaaatt gcgtataatg tcactttatc aatgcagtgt gtttccaaag cttcattgcg
     2581 cagggttatc ctgaaagtgc ttcccggtaa aacactaccg ggtagggcat cgatatcgcc
     2641 atattgcatt gatggtagaa tggcattcga aaaaccttct gcatcggcat aatgtgtagt
     2701 tgcggtggag tcgatataaa atgtaggaca agatccccag catgctttgg gaacagtaat
     2761 gcataccact ccaaacaaaa tatccaaacc ggtcatgatg gtgatagccg agattctacc
     2821 cttattatcc tgaacgggat tgttggtttc aaataatgca atgtcatcaa taggaatact
     2881 cagatgatcc tgtgtaatca gttgcctgtt aaaatcatat tgtgctccga aaccggaaat
     2941 ataggtttga gcactgtcaa cctcccaggt attataaaaa atgacaatgt tgccattttt
     3001 catatgtgct tttagccagg gcgttcctgt ggcaaactgc gtagaatcgt gtaatagggt
     3061 atttacattg tagtaatcct tcctgaattg tgtggtgccg caagagctaa tgcctgctat
     3121 aactaccagt aacagagtta tatgaaataa tcttcgtata tgaagtttat ggagcatatt
     3181 ataaagttga taataattac catagcatgc aaatgatttt tatcactgat gggctgaaat
     3241 ctatgacccg tgaaacacct aacgcaaggg aaggtgagcc tgaagggcgg ttctgaaagt
     3301 atcacctatc ggaataggct ttccggcgat gatcaacctg tttgcttcga tggctgtaat
     3361 tttttcggtc gctatgataa atgattgatg tatccttaat aaagcaattg cgaaagcaca
     3421 gatgcatcat ctatggattt ttaagcaaga aatttgcgta tttatagaaa tattcagcaa
     3481 attaccacaa gccccgacga tagaatactg gtgattattg gagcggggca tgcacaaata
     3541 ttaacgcacc tgtttgcttg ctctccggct tacaaactgg tgcgtttgga atcgctgcaa
     3601 caagtgcagg gaaaaaagtt ataagtgcat tactgcatct gctccacact gcgctttaca
     3661 aattcggtga gcgcagtgcc tttgagcata ccttgcgata ataatgcaag gtcgtgaagc
     3721 tgacgggccg tatttaaacg atgagtttca tcaagttgca gcattttatt tactacggga
     3781 tgattggtat taattaccaa attgaattga tcaggcattg atgctgcgaa atccatgccg
     3841 ttgaggtgac tcatctcttt catacgacgc atgaattccg gtttggttat agttacgggt
     3901 tgatcattcg gcgacattgg tgtgagcaca acagtgccgg cattggcatc gacaacttgt
     3961 tcgaacaatt cttttacact ggtttgttcg gtatcgctta aaacagattc aattttttca
     4021 tctttatcga taagttgatc aatagtatcg gcgtcaacgc gtttgaattg tacgccggtc
     4081 attttttgtt cgatgaaaga aatgaagtga ttgtctatca cattatcgat ttccaacaca
     4141 tcatatcctt tgttgattgc tgcgtcaacg tacacatgat gatcggttac attattggta
     4201 taaagtgcaa tcacctgctt gtttttatcc tcttgagcag gagcaatatg tgctttgtat
     4261 tcatcgaggg taaagaattt cttttcagta ttttgcagca gacagaattt ttcaccacgt
     4321 tcaaaaaact tctcatcgct gagcatacca tatttgatga tcacgttgat gttctcccat
     4381 ttttcttcga atgctgctct gtctttttta aagagctctt cgagtttttc ggctactttt
     4441 ttggtgatgt agctatttat tttttttacg ttttgatcac tctgcagata gctccgcgat
     4501 acgttaagcg gtatatccgg cgagtcgatc accccatgca acaaggtgag ccattcgggc
     4561 acaacagcat ttacttcatc ggtaacaaat acctgattgc tgtacaggtg gattttattt
     4621 ttctgtattt catagctttt tttaatgcgt gggaaataca gaatacctgt gaggttgaat
     4681 ggataatcta cattcaaatg tatccaaaac atcggcacct cggcatacgg atataattcg
     4741 cgatagaaat tggtataatc ctcgtcggta agctcagcag gttttttggt ccacgccgga
     4801 tttgggttat tgataatgcg tggagcagtt acttctgttt catttccggc ctcatctttt
     4861 tccttggtaa cagattcacc aaattgaatg ggtacaggca aaaacttaca atatttatcg
     4921 agcagagtct gtatgcgatt atcttcgagg aattctttgt tttcttcgtt gatgtataaa
     4981 atcacatcag ttccacgcgt ttctttttca acaacttcga gggtgaattg cggactgcca
     5041 tcgcaagtcc agcgcacagc atcatgtcct tctcttgccg agcgagttac caatgctacc
     5101 tggtcggcaa ccatgaaagc gctatagaaa ccaaggccaa aatgtccgat gatggattta
     5161 ccatcttcca gtcctttgta tttatccagg aattcagagg cactggacaa agccacctgg
     5221 ttgatatagc gtttcacttc atcgccgctc ataccaattc ctctgtcaga gatggtgagg
     5281 gttccggctt ctttatctat tgaaatacgg atattagtgt ctcccagttc tccgccgatc
     5341 tcacccatag aactcaattt gcggagcttt tgagtagcat ctaccgcgtt ggcaaccaat
     5401 tcccgaagaa agatctcatg gtcgctgtaa aggaattttt tgataatggg gaagatattc
     5461 tcagtctgga cggcgatatt tcctgtttct attgacatag cgttattgtt tgtgctttcg
     5521 gcatctcaaa gcctgtgcca tggggatatt gctgacattt tgtcgctgcc gatgcatgtc
     5581 atctcctgaa attgctcaaa cccttatatt tgggcatgcc tacctcaggt cgactcaacc
     5641 gcaaacagat tgaagcagag atcagcggac tgttacacac ctcttgggac ctggtgagca
     5701 agcgcaattc tgagatagcg gaattgtcgt cgaaggcact cgctcttgcc gagcaaatca
     5761 acgataataa tctgaagggt ttgtgtattc tggaaggagc cctttatcag tgtttggtga
     5821 aaagtgatta tcacttgagc atcaaaatgt ctgatgaggg ttatgcgatg atgaaaggag
     5881 agtttaaaaa aagatatgct ccttattatc atctgaatgt tggacgcaat taccatttca
     5941 tgggcgatca cgtttcggca caacataatt atctggaatg tgtgaaatgt atagagtcaa
     6001 tgagcgaaca aaatcaattt gaattaaaat ggcttgctca tgcctactac aatttatata
     6061 tcctgttcaa tttctccggt gccgaatttg cacaagcaga gtatttgcaa aaggcattaa
     6121 ccatttataa gcagatcggc gatgttggtg gtgaagggaa ttgttacaat tcatatgcag
     6181 tttattattt cagattaaat aattatacgc aagcatatga aaatcttcag cgcgcatatg
     6241 cattggcaga acaggaacaa gctacttcgc tcctttctat ttattctgca aatattggct
     6301 tggtaaaagt gaaaatgggt gaagtagaaa caggatttac ttttttcgat aaggcgcagc
     6361 attacgattt ggaattagat agcccctatc atcacgccca tactcaaaat caaatgggtg
     6421 aggcatttat tattttggaa cgttatgaag aggctatcga acgtttcgcc aaggcattgc
     6481 aatatttcga aagcattggc gtaaaacgct ccatgagtaa tacttacgag cagatggcca
     6541 aagcgtatgc cggactggga aatcatgagc gcgcatacca ctatcaacaa gcctatgctc
     6601 agtcattaaa agatgtattc agcgatgaga aaacatttgc cgttgccaaa gctcgcaatg
     6661 attttgaatt ggaaatgcgc gatcaggagg caagaaaatt aagactgaaa aataaacaga
     6721 tagagcaata cgcaaggcag ttggaaatgt cgaatgatga attgcgacaa tttgcacacg
     6781 ttgccagtca cgacctacgt gaaccattgc gcatggtatc gtcatacgtg caattgctcc
     6841 agcgctcaat ggatataccc ttgaccggtg accagcagga attcatgaga tatattttgc
     6901 agggcactca tacaatgcaa aatctaatca gtgatctgtt ggccttagcc gccatcagta
     6961 acgtacgcaa ttcggagtta gttgatctga atattattgt gcaacaggtg aaatcaaacc
     7021 ttcaaccggt aatagaggaa agaaatgcag taataaaaag caccttgctt ccggcagtaa
     7081 tagcagatga aacacatatg ttgcaattgt tcctgaatct gatttccaat ggtattaaat
     7141 ataatacaag cactcaaccg ctgattgaaa tttcatttgc tacttcgggc agatattatc
     7201 aattctccgt tgccgataat ggcattggca tacctgcaga atacaaggag aaaatctttc
     7261 tcatcttcca acgacttcac tccaaagagg aatatagcgg caccggtatc ggactggcaa
     7321 tttgtaaaaa aattgttgat caggccggcg gcaccattaa ggtatcgtcg cgcaagggtg
     7381 gtggcagcat atttacgttt acattgccgg tgattgaaca tcagccggaa agtgtataat
     7441 cgctttaatt cccggcctta tttatctttg caagtaagcc attttatcac gcaaaactaa
     7501 aatcgggcat attttttgta ataaagcctg aaagattagg tacatgagac ttctagccgg
     7561 tatagccttt tgtttaatgt gtgcatcctg cgcatcgaca caggaattgc aatacaaggg
     7621 atttgatgat tttacggtta cgaatattct caaggaacca aaagtgcaaa tggacctgaa
     7681 tctgtataat cccaatccga taggtggcac cattcgcaat atggaaataa atattgccgt
     7741 agatcaaaaa acgcttggaa gtgtctcttt agatgataag gtgcgcataa aaaaacaagc
     7801 agagttcacc atgcctatat ctttcacgac aagcgtcagt gaaatgggcg gaatactgtc
     7861 tgcaggattg aaatcgtttg ttggtcagga tgaaatgcct ttggggatat caggagagat
     7921 taccattcag aaatttctgg tattcagaaa gacattttca tttgaataca aggatggtat
     7981 tgatattcac gacatcattc agaaataaaa attccattat attttttcca ggtatgctgc
     8041 gggtactttg atgagtaatg cttgctgtat cacatcaatt ctcagcagga ataatttttc
     8101 gttttctata gcaactaatt cgccaatggt gcctgccatg gggccgaagt tgatttttac
     8161 ctgtgtgcct attgcaaatg aatcaggatg cacctcggta tgaaactcgg cattcaacag
     8221 cgattttatt gttgaaatat ccgattccgg catgcgagca ggtttacctt cgcgataaat
     8281 aaagtttgcc acaccgctaa ccatgcgtac attggtgagt tgttcttcta agcaatgcac
     8341 aaaaatatat ccgctgaaca tgggaatgga aacgatcttt tttctgtcac tccattggcg
     8401 aacagatttt atcaaaggca agtaggaagt aatgccattt ttttcaaggc gttctgcaac
     8461 ttttttttca ctgcgtgagc gcacatatgc cacataccaa tgcgccgcca tatttaattt
     8521 ttttgagtga gatgttgcgc gcgcagtgca taaatgcgtg aaatcacatc aacaaccata
     8581 cgtccttctt cagctgttgt ggctattggt gcttcgcgat gtaaaacatc taatacgttc
     8641 tgaataataa aatggtgatt ggcagcagaa ccaatgtatg gaccataatt atttggcgca
     8701 gcggtaggcg ctaattcagg cattacataa tcttgcacat gacaataggt tacttcattc
     8761 atgtattgcc cgcctaagca tactgtccct ttctcaccta taatacgaat ggtgctttca
     8821 aaatttttat catacacggc agtactgtat tgcaagcttc ctaaggcgcc gtttaaaaag
     8881 cgaaaatgca ccatcccgct gtcttcgaaa tcggtaaggt gtttatgctt gtagtcgtcg
     8941 aaaatgccgt gaatatctga gatatcgcca aacaaccaaa gcatggtatc aatgaagtgt
     9001 gagaattgag taaacaatgt tccgccgtcg aaatcacgag tgccatgcca tgaatcgccg
     9061 gtataatatc tttcatcgcg attccagtag caattcacct gcaccatgca aatttttcca
     9121 agtgcatgag tgtctaacat tgattttatc cagaccgatg gcggagaaaa tctgttctgc
     9181 attacacaga atacttcctt gtttttatct tttgccagtt taataacagc atcacaatct
     9241 gcaacctgca aggccatggg tttttctatc accacatgtt tattgtgttg aagacatgac
     9301 agtgcatgtt cggcatgtaa tccatttggc gaacaaatat ttaccacatc aataggtaaa
     9361 ttgctattca gcatctccgt gatggaagta aatactgcaa caccttcatc aattcctgct
     9421 aacttttgag catcaacatc gcaaattgca gttacaatcg cacccggtgt ttcacgaata
     9481 actgcggcat gtctttttcc gatatgaccg tgaccaatga ttgcgaaatg tattacaggt
     9541 gacgacacga tgatcttaat tatttaattg ttgtactctg ccgccggaaa gtttataatt
     9601 aatccctgaa atatcgcaaa cggcatgacc tttagaatcg aagattaatt taccgccatg
     9661 ggcactcatc catccttttt gacgagcagg atttcctgtt accagtgcat aatcgggaac
     9721 agattttgtg acaactgcac ctgcgccaat gaaagcatat ttcccaatag tgataccgca
     9781 aataattgtt gcatttgctc caatgctggc accttttaat acacgtgtaa gttgaaattc
     9841 atttttacgt tcaacagcac ttcgcggatt gatgacattg gtgaatacca tgcttggtcc
     9901 aagaaagaca tcatcttcac acatcacacc ttcataaagc gatacattgt tttgaatttt
     9961 tactcggttt ccgatctcca ccctgctggc gatgaataca ttttgaccaa tcacgcagtc
    10021 ctctcccaca atagcctcag gcataatatg cgtaaaatgc caaatttttg tgcctttacc
    10081 tataatggca ccatcatcaa caacggcgga cgaatgtatg aaatgcgatt gcttcgacat
    10141 gattatgggc aaagataagt gtttgaggag aagcattatc ttcgcgctat gatgcgcata
    10201 aacggacgct ttgctatgat gatcatgggt ttgggagcct tgctgacagg gtcctgctct
    10261 cgcgatttcc ttggcgagga ggctctgaat ttggcacctg agacttacct cgttacagat
    10321 accattatcc gtttcggcga cgacaggctg gaaagtgagg ttacccttca gtggtggggc
    10381 gatgacgaag atggctttgt aacaggctat gagtttacat tcgatgaggt gattacagac
    10441 gctaccatct ggcagtatac tgagtcgcag gacagtactt taattctggc acctccgccg
    10501 ggagaggatt ctgccgattt cgtattccat atccgggcga tagacaatca tgatctagct
    10561 gacccgacac ctgcagcttt aacagtacct gttaaaaaca gccctccggc gataattatc
    10621 attcccggcc tcaatacccc taaaaaatca ttccctgctc tgcgctatta ctgggaatca
    10681 agtgaccccg atggggctca gaacctgtcg catgtagaat tatttttcaa tgacaccacc
    10741 ggcacgccat atatactgcc gtcgaccgta acgagtgcca tttttcaggc acaggatcct
    10801 acagcattga cacttaattg tgaggtgttt gcaaacggcg gcacgattgc ggaggacatt
    10861 ttgatgaatg gattgatggc aaatgcctgg aataaattat atgtgcgtgc agtagatcaa
    10921 agcaatgcaa aaagtgcttt cgccattgca gattctgtat tcgtgaaaca ggtgcacagt
    10981 acggtattgc tggtgaatgg atacagcact gctacaaatg aagcgtttta tatgtcgcat
    11041 ctcagtgcca acggaattgc agttgtcgac acgatgcaga tttttgaaac tgtgggaggt
    11101 gtgtatacac agcaatcggc agacaacctc actcaaggaa gaatatttgc catgtttgat
    11161 gctattatct ggtttagcaa taatgccgat aattcatttt cactcgcgca aaaaacaact
    11221 gaatcttttt ttaataatgg cggtaaaatg ctgatgagtg tatacatctc ttcatcattt
    11281 gatccattga gtaatttcct cgatttcaca ccaattgctt cattggttaa tccggtagat
    11341 accacattaa ttctcgatct gggcgcatcg ctttcggctg aagttgccga ctggcctgat
    11401 cttcagggga cttctattgt gggggtggtg aagccaataa acttgcaaat aggtgctaca
    11461 cctttatata ctgctacact tactgcaaaa gataatgttg cgttgacctt tacgccatgg
    11521 acaggtgtgt cgacagtaat ggctagtaaa actgatggtg cagggcaaac gaattttgtg
    11581 atttctacac tcgaattaaa taaactggat gggctgttga acatggacag cttttttgaa
    11641 aaagtgatga tagatgaatt cggattttga gatgcatgtt gcaggtggtg atcatcgcat
    11701 agccattttt gcatcaggtg ccggaactaa tgcggaaaat tgtattcgtt atttcagaac
    11761 acatgcttct atatatgtag cattaattgt taccaataaa cctacagccg gagttatcga
    11821 agtggcagag cgatatggta taccatatat gattcaggaa acctgggatg atgaaggtac
    11881 cgaattatta ttatcgcgat tggcacaaca tcgcatttca tttattttat tggccggttt
    11941 tttgagttta gtgccgaatg aattaatcag gaaatttacc ggtcgtatta tcaatatcca
    12001 tcctgcattg ttgcccgcat ttggtggtaa gggcatgtat ggaaaacgcg tgcatgcagc
    12061 agtgtatgcg gcaggagttg gtgaaagtgg catcaccata cacgaagttg atgaagaata
    12121 tgaccatggt aaaattttat ttcaggcgaa ggtggcaatt gatggccagg atactccggc
    12181 ctcaatagaa gaaaaagttc gagcacttga atacgctcat ctccccgctg tggtggaaaa
    12241 attgttgcta acaggcagag tttgacctca taccgtaatt gacacgtatg aatacactgc
    12301 ttttctgctg tagaggcggt aattttgcac tttctttcac attatgcaaa tcgaacacca
    12361 tcctgttaag atcaagtcgg ccttgatctc tgtttattac aaagacaagc tagagccaat
    12421 tattcatgca ctgcaccgac ttggggtagt attatattcg accggaggaa ctaggagttt
    12481 tattgaagat ctcggagttc ctgtgcaggc tgtggaagac ctgacgctgt atccaagcat
    12541 tttgggtggt cgagtgaaaa ctttgcatcc aaaggttttt ggcggaatcc tagccagacg
    12601 cgcgtcgaat gaagatcagc agcaacttac ccaatatcat attccggaga tcgaccttgt
    12661 gattgttgat ttgtatccgt ttgaagaaac aattcgacat accaccgacc atagtgagat
    12721 cattgaaaag attgatatcg gtggcgtatc actgatacga gcagcggcaa agaattatca
    12781 tgacgtgctc attattgctt cgaaagatga ctatagtgcg ttgcaggata tacttgatgc
    12841 cggaggtgaa acgaaattgg gacagcgtat gtatttcgcc aagaaggcat ttaatatcac
    12901 gtcgtcatat gacagtgcca ttttcgggtg gatggaccat ggggagcatc ttgacctgcg
    12961 ttatgcgcct aaaatcggca tgcgttatgg tgaaaatccg catcagcaag cggcctttta
    13021 cggtcatttg agtgaggtgg ttgagcagat acaagggaag gaaatatctt ataacaacct
    13081 agtagatatt gaaagctgta tttacctgat ggaggagttt gaagcatcta aggaagcagg
    13141 gaaagcaacg tttgccatta tcaagcatac caatgcatgc ggactggcaa ctgcagacag
    13201 cgtagaaagt gcctgggata aggctttggc ggcagatccg gtgtcggcat tcggaggggt
    13261 tttgatctgt aaccaagcta tcgataaagc cgctgcaggc aagatcaatg agttattttt
    13321 cgaggtcatc atagcgccag attttgatgc tgaagcattg gcacttttac aatcgaaaaa
    13381 gaacaggatc atattgcgtc agcgcatgtt caaattcccg gtgaaacagt tcaaaactgt
    13441 attgaacggc gtattgatgc aagatcgaaa tgtgaaggca acaagagctg atgaattgat
    13501 gaaggttacc gatatacatc caaccgaagc gcaaatacaa gatctgttgt ttgctgaagt
    13561 agttgtgaag catttaaaat ccaatggcat agccttagtt cgcaatggtc aattaatagg
    13621 atgcggcaca ggacaaacaa gtagggtaga tgcattaaaa caagctttgg ctaaagcagc
    13681 attatttggt tttgatgtgc gtggttcagt gatggcaagt gaggcctttt ttccattccc
    13741 tgattgcgtc cagattgccc atgaggcggg catttcagct gttattcaac cgggtggaag
    13801 tgtgcgtgat aaagacagta tcgactactg caatacacat ggtttaccta tggtgttgag
    13861 tggcacacga cacttcaagc attgatattt tttatgcttc attttaatgc ttacttttga
    13921 ttgataagca gagataattt ttgcgaataa ttttcgttcg taaaaaaagt tagttttatt
    13981 ttgataattt atgtgaaatt ccttcgcgga tacctaacat aagttaattt tgcacactta
    14041 tatctttacg agcagagcat catgggcata ttcaactttc tcactcagga aatagctatc
    14101 gacctgggca ccgccaatac cctcatcatt gcacacgata aggtgatggt ggatgaacca
    14161 tctatcgtgg ctatcgaccg caaaactgaa aaagtgattg ctgttggaaa gaaagccatg
    14221 cagatgcatg aaaagaccca tgagaacatt aaaaccattc gaccccttcg cgatggtgtg
    14281 attgccgact ttcaggccgc tgaatctatg atcagaggca tgataagcat gatttttccc
    14341 aaaaacaggc tgtttaagcc ctctttggtg atggttatct gtattccatc aggtatcacc
    14401 gaagttgaaa aacgtgccgt aaaagatagt gccgaacaag ccggagcccg cgaggcatac
    14461 ctgattcacg aacctatggc agcagccctg ggtatcggtt tggatgtgca ggagcccgtt
    14521 ggaaatatga tcatcgatat cggtggtggt actaccgaaa ttgctgtagt cgccttatcg
    14581 ggtatcgttt gcgaccagtc aatccgcgtt gccggcgatg agtttacggc agatattgtg
    14641 gattacatgc gccgtcagca caacctcctc atcggagagc gcaccgctga atctatcaaa
    14701 atcaatgtcg gcgcagcact taccgaactc gacgatgctc ctgaagatta tcaggtaagt
    14761 ggtcgcgact taatgaccgg tatccctaaa caattggtag taacctattc tgaaatagcc
    14821 cattcgcttg ataaatctat cagcaagatc gaagaggcca tcctcaaggc cctggagacc
    14881 acaccccctg aattagccag tgatattcaa cgcaacggta tctatcttac cggcggtgga
    14941 gcattattgc gcggcctcga caaacgtatc agccagaaga caaagctcgt tgtgcatgta
    15001 gccgatgatc cgctgaaggc tgtagtgcgc ggtacaggga tagccctgaa gaaccgggag
    15061 cagtatgcgg ctttactgat gagctaaacc gccaccctgc ataatgcgta gcttcttctt
    15121 attcatcatc cgtcattatg ccatcttcat gttcctgttt ctggaattgt tgtcgtttta
    15181 ttttatagtg acacataatt cctatcagca aacatatttt ataaataccg ccaatacctt
    15241 gtcgggaaat gtgctgaaca gctacggcaa ttttcaagaa tatttttcac tgcgcgaagt
    15301 aaatgatagt ctgatgcatg aaaatgccat gctcagagaa caattattat tgagtcagat
    15361 tgctgatacc gttaaccata ccgtaagcga cagcaccgga aaaccgctct acagctacat
    15421 tccggcagaa gtgataggga atacatttac tgaagcaaat aattatatca ccctcaacaa
    15481 aggttcctcc gacgggattc aagtgaacat gggtgtgatc acctccggcg gaatagttgg
    15541 cagagtggtt aaagtatctc ccaatttcag cgtggtgatg agtgtgctgc atacgcagtt
    15601 tgtaagtcgc gtagccgtaa aaagaagcaa tgctcaaggc cgactaacat gggatggcaa
    15661 acgcccgaca catattgatg taattcaagt atcagagccg ggaacaatta aaaagggcga
    15721 cagcattatc acaaccaaag tatcgccaat gtttcctccg gatattatgg ttggaacatt
    15781 ggagagcata gggaaggatc ccggaagcaa ttattataca cttgatgtaa aactgagcac
    15841 caatttcagc tcattaaaat atgtatatgt ggtgaacgag atcatgtcga aggaacggaa
    15901 tgatcttgaa aaccaaattc aggatgcagg taattaactg gatattgcgt gttgttggac
    15961 tggtctttct gatcatgttt caggtattgg tgcttaacag gctcaatgtg agtgaatatg
    16021 tgcatccata cgtatatcca atgtttatca ttctgcttcc attcgacaca ccaaggtgga
    16081 tgttgctggt ggtagcctac ttgattggtt ttaccatcga catgttcaac aatacccccg
    16141 gcatgcatgc agcagcctgt gttttcattg cctatttcag acctcaattg atcaagtttt
    16201 ataccccgat taccggttat gaaagtgtaa catcgccatc catatcagag ctcggtgtga
    16261 tatggtttac catgtttacg gctacaacca ttttattgca tcatgctgtt tattttctga
    16321 ttcaattatt ctccatcaac gaccttgggt ttttaatgat aaggataggt ttaagtacgg
    16381 tagtttcaac cttgttgatc gttattctgg cattcctctt tgctaagcgt aaagtacgtg
    16441 caaaataagg atgtaaattt ttgctttgct ctcgaaaaca tttctgaatt ttgtaaaagg
    16501 aattgttgtt acaggcgtta ttgaaggaac aacatcatta tttccttcca acacgatttc
    16561 caccaccaat caatggccgt aatagaaaat ctcaaacctc gcggatatgt catacaagcg
    16621 gtgatagcgg caatttttgt cgtgttcatt gttcgtcttt tctatttgca agtgatcgat
    16681 gatacctata agcagatagc cgacgaccgc ggtattcgca aagagcaggt agatcccgac
    16741 agaggtcaga ttttcgatcg caatggtaag ttgatcgttt acaatgaacc ttcgtacaag
    16801 ttgatggtgg tgccatcgca agtgaaggat attgatacca ttcgattttg tgagatcatt
    16861 ggtgtcgata aggagtattt cgagaaaaag atgaaagagg cgaaaaactt ttctcgtgta
    16921 cgtgaaagtg tattcttatc tcagatttca gcagaggatt ttggaagaat tgaagagtat
    16981 ctgtatgaat tcaaaggctt ttataccgcc atcacacctg ttcgccggta tacatatcct
    17041 gcagccggtc acttgttggg atatattgcc gaagtagact ccaacgacat caaaaagtct
    17101 gatggttact atcgcaatgc cgatatgata ggaaaaaccg gcgttgaaaa gagttatgag
    17161 aagctgttaa gggggaagaa gggatatcat aacattgtta tcgatcgctt cggtcgcgct
    17221 gtgggccctt tgaataatgg gagtgaagat gtggaaccgg tggcaggaaa gaatctcacg
    17281 ctcactatag acatagaatt acaatattat gccgagcagc tgatggtagg aaagagagga
    17341 gcattggtgg ccatagaacc ttcaacaggt gaaatactgg ccctggtaag ctcacctact
    17401 tatgatccga atatgctgtc gggaagtgcc cgcaataaaa atttcgccaa actggcactc
    17461 aacaaggaaa atccgttgaa caacagggcg ctgagtggat attaccctcc ggggtcaact
    17521 tttaaaccgg tgatggcagc tcttggcatg caacaaggtt cactcacgtc aactacggga
    17581 tatagctgtc cgcatggtta tgtaatgagt gggcatactg tcgactgcca caatcaccct
    17641 tatcctgcca atgtggaact gggaatcggc cattcatgta atgcctattt ctgttatgcc
    17701 ttccgctatt ttattgaaga tcattcaaac ccggcagagg gtatgcgcga ttttaaagca
    17761 aaactcgcct tatggggtat tggtacccga acaggtattg acctgccaaa tgaaagaaat
    17821 ggaaacgtgc caagtcctga agactacgat aaaatatacg gtaaaggaag atggaaggct
    17881 tcgaattgtg taacactcgg tataggacaa gacagactta ttttaacgcc gcttcaatca
    17941 gctaattcaa tggcgacgat tgccaatggt ggttattttt attcaccaca tgtattaaaa
    18001 tttgctgatg gggaagattc gttaatgaat gcttttacca tacgtaaaga gacaggtatt
    18061 tcggattcca tattcaaata tgtgcgtcat ggtatggcag gagctgtaaa ttttggtaca
    18121 gcaaaagttg cacatatcga ttccattcag gtttgcggta aaacaggtac agcagaaaat
    18181 ccgcatggaa aaagtcacag ctggttcagt tgttttgcac cggaagtaaa tccgaagatt
    18241 gccgttgcca ttattgttga aaatggcgga tggggagcaa cctatgctgc gccaattgca
    18301 agtttggttg tagagaaata tatgaacgac agcatcagtg caaagcgaaa accactggaa
    18361 gagcgtatga tgaaatcagt tttgatactg gatagagaag ttgttccacc aattcaaaat
    18421 aatagcaatc aggacactac aaaacaggtt caggctgcac ttttacctaa agagcaatga
    18481 gagaaaaaaa atccatcatg gaaaatgtag atcaaccttt gttatggttg tatctgtcct
    18541 tgatggtgat tggtctactc aatgtatttg ccgttagctt tagtgatgat gtgaaatttt
    18601 ttgacctctc cttaccacat ggaaaacaat ttttgtggat gggaatttca ttggttgttg
    18661 gatttagcat tctcaccttt gaaagcagtt tttttacaaa gttcgccatg gtgttatatg
    18721 gtgttactat agccgtatta attgtcacat ttgtagtagc cgtagacatt aacggcgcac
    18781 ggtcatggtt gcaggtgggt tcgtttcagt ttcagcccgc ggaattcggg aagacaacta
    18841 cggcactgat gctcgcgaaa tttttgagta tcataaatgg tcagccgcga tcctttaaaa
    18901 ataaactact gagctatctc atcattggag tgccgatggg gattatcgtt ctgcaaagcg
    18961 atgtggggtc tgcattggtt tatgccagtt tagtattggt tgtttatcgc gaaggatttc
    19021 cggtgtatga ggtgttgttt gtacttgcca ttggattgtg tttcgtgttc gcactaattt
    19081 ttggcgtaga tccggtggcg gttgtgctta cattcatcat ttggctatac attgtattta
    19141 attcattcaa actggtgcga aaagaaaaaa ttaaggggat tgttttttta gtgatttttt
    19201 ctgcaggcgt attcttcttt ttgataggtg gcgaagtatt gggcatggat acacatattg
    19261 cagcagcggt tatgctcatt gccatgatag cggcgctgat taatgtgaga aaggttcgtt
    19321 tttttcaaat gaatattccg gtagtattat ttgccatttt gatggtattt gtgatgtttg
    19381 gtgtagaaac tatcacaaca gatattcttg aagattatca ggtgcaacgt atcgaaacat
    19441 tattgggcat gtctgatgat gccgatgcgg aatataatgt tactcaaagt aaaatgacaa
    19501 tcggttcggg aggcgttttt ggtaaaggat accttcaagg cactctcacc caatctgaac
    19561 aagtgccgga gcaaagcacc gattttattt tctgcactat aggtgaagag tggggtttct
    19621 ttgggacatt catttttctt gtgatctatt tgctatttat tctccgcgta atttttatcg
    19681 ccgagcgaca acgatcgcct ttttcgaggg tgtatgctta ttgcgtggca agttttttct
    19741 tcctgcaaat catgattaat gtgggaatga ccattgggtt aattccggta atcggtatcc
    19801 cgctgccctt tatctcctac ggcggatctt cggtgctggc tttcagtatc atgatattca
    19861 tcatgctccg gttggatgcc gataggctat tcatattgcg ttaagtaccg aaataaggct
    19921 tctgattgtc ataccgggct gcaagcacag ttgccgtgaa ttgcaatatc tttgcacaaa
    19981 attcacatca atggaaacct tccatatcgg catacaagga ctcacgcttc aacaaataag
    20041 cgatatcatc aaaaataaaa tacgcattgc attaagcgat gaggcgcgtg agaaaattca
    20101 gaaatgtcgt acttatctcg ataataaaat tgcaagtcag gataaggcaa tttatggtgt
    20161 taataccggc ttcggaagtt tgtgtaaagt gaaaatttct gatagcgatc tggagcaact
    20221 acaatataat ttaattcgca gccacgcttg tggaatgggt gccgttattc cggatgaaat
    20281 ttgtaggatt ttattgctcc ttaaaatcca aaacctggct ttcggccgtt cgggtgttgc
    20341 cgttgaaacg gtatggcgac tcattgattt ttataacatg gatctcctgc ctgttatcta
    20401 tgaacaaggt tcattaggtg catcgggcga tctttgtccg cttgcacata tgagtttgcc
    20461 attgattggt gaaggagaat tgaaagtaaa cggtaaaatc agacctactg cagaagtatt
    20521 gaaagaagcg aaattgtcgc cattgaaatt gcaatcaaag gaaggtcttg cactattaaa
    20581 cggaacacag tttatggctg cgcatgccgt gtatacattg attaaagctg ataatattat
    20641 tcgtcagtcg acatacattg ccggagtttc tctcgaagca tacgattgca aatcggagcc
    20701 gtttaccgat cagattcatc aaattcgcgg gcagcatgga cagattgcta ttgcgaaggc
    20761 ttttatgcat ataatgcagg gttctgaatt gtttttcgct gagaaacagc aagtgcagga
    20821 tccgtattct ttcaggtgta ttccgcaggt gcatggtgct tgttatgatg ctatacaata
    20881 ttgtaaaact attgttgagc gcgagatcaa tggtgtaaca gataatccga atgtgtttgt
    20941 tgaggaggat atgattttga gtggcggaaa ttttcacgga gagccgctcg ctttaacttt
    21001 agattttctg gctatggcat tgagtgagat gggcaatatt tctgaacgaa gggtttatag
    21061 attattaagt ggagcgcgtg gactgcctga atttttgacg cctgatccgg gattgcattc
    21121 gggattgatg attccgcaat atgctgcggc gagtattgtt tctcaaaata aacagttatg
    21181 tactccggcg agtgttgatt ctattagcag ttgtaatgaa caggaagatc atgtgagtat
    21241 gggtgccaat gcggcaacaa aatgtttgcg tgtggcaatt aatgttgaac gtattctggc
    21301 tattgaattg ttgactgcag cgcaagcctt cgaattccgt cgccctgcta aaacatctcc
    21361 ggcactggaa aattacattg cttcataccg ccaacacgta accttcaaca ccgaagaccg
    21421 cttcctccac aaagacatgc tcactacaga gaagtactta caaatggtgt gataatttca
    21481 cgcagaggcg cagagacgca gagttttttt gaacatgttg tattgtggag cgacaatagc
    21541 gtaagagctt agggtggata aagttttatt aaattgctta ttaattggca aaatttggtg
    21601 attgtttcac ccttaaattt tgttatgcat attaatcagc tttccggtgt tatcgtggat
    21661 attttaatcc acatgcacaa agaacttggc cccggtttat tcgaatcagt ttatgaagag
    21721 gtgctggcat ctgaacttga taaacgtgag attgggtata ggcggcagca ggtaattcct
    21781 attgtttatg atgatgtagt gttagtagaa aatgccttca aggcagatat tattgttgat
    21841 aataggatta taattgaact taaatctata gatcaattat caccggttca ttataagcag
    21901 ttgttgacat atttacggct cacaggaata aagctcggcc tgcttgttaa tttcaatgaa
    21961 gtcctaataa aagatggcat tcacagagta gtgaataatc tctaaactct gcgtctctgc
    22021 gcctctgcgt gaaatcttac agtagcttaa ccccaacaat acggagctga tcgttgtcga
    22081 cttcgccggg ggcgtccatc atcatatcgc ggccgttgtt gttttttggg aatggaatta
    22141 catcgcggat agtatctcca cccgccatga gcattaccca tctatcgaag ccaaacgcac
    22201 aaccaccatg cggcggtgca ccatatttaa aagcattcac cataaatccg aagcgacgtt
    22261 ctttttcttc ctcagagaag ccaagaatct tgaatatttt ttcctgaagt tctttgttgt
    22321 gaatacgaat actgccactc aaaatctcat tgccattcat caccatatca taactctgag
    22381 cacgcacgcg caaaggatcg ctatccatat attggaaatc atcgggatgc ggcgagcaga
    22441 atggatgatg cgcaaaaatc ggttcacccg tttcttcatc aggttcgaat aaaggcatat
    22501 ctaccaccca aaatacactc cacgcatttt tatcaatcca attttctcgc ttcgccattt
    22561 ccaatcgcag atcgccgagt actttacgtg ttttcttttc tttatcggca actatcagta
    22621 ataaatctcc tttccctgca ccaactgatt cgccgatagc cattaattgt tcaggcgtaa
    22681 agaatttatc catagatgat tttacggagc catcctgact gaatttaata tatatcaaac
    22741 caccgactcc acgctgcggc gctttaacaa attccgtgag ttcgtcgatt tgtttgcgtg
    22801 aatattcact gcatcctttt gcaacaatac ccgcaaccaa tccattggaa gccagcacat
    22861 tattaaatac accaaattca cttccgccta caacggcatt taagtcttga attttacaat
    22921 cgaagcgcaa atccggttta tcgctgccgt aatatttcat cgcatcacgg taaggcaagc
    22981 gaataaaatc cggaaggtca atatttttta ctttcttaaa tacatgcttc gtcattcctt
    23041 caaacatatt ccacacatcc tcctgacgca caaagctcat ttcacagtcg atctgcgtaa
    23101 attccggctg acggtcgcca cgcaaatctt catcgcgaaa acattttgca atttggtagt
    23161 agcgatccat tccgctcacc atgagcaatt gttttaatat ttgtggcgat tgcggcaatg
    23221 cataaaacaa tcccggctgc atgcgcgaag gcactaaaaa gtcgcgcgca ccttcgggag
    23281 tggatttt
//
LOCUS       DAOQOU010000036        23058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1060_length_23058_cov_5.990045, whole genome shotgun sequence.
ACCESSION   DAOQOU010000036 DAOQOU010000000
VERSION     DAOQOU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23058
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1060_length_23058_cov_5.990045"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1171
                     /locus_tag="PLM90_05035"
     CDS             230..1171
                     /locus_tag="PLM90_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HMZ88765.1"
                     /translation="MHNRITALFGIEYPIIQAGMIWCSGWKLASAVSNAGGLGLIGSG
                     SMYPDVLREHIQKCKAATNKPFGVNVPLLYPDIDKHIQIIIEEGVKIVFTSAGNPKTW
                     TSMLKQHGIKVVHVVSSRKFALKCQEAGCDAVVAEGFEAGGHNGREETTTLCLIPSVA
                     EAVTIPVIAAGGIGDGRAMLACMVLGAEGVQIGTRFIASIESSAHDAFKQTVIHADEG
                     STILTLKEITPVRMVRNAFYDMLSAAYARCASADELRTILGRGRAKKGMFEGDMEAGE
                     LEIGQVSATVREILPAAEILKDIWEGYLVAKSGLTKQ"
     gene            1384..1740
                     /locus_tag="PLM90_05040"
     CDS             1384..1740
                     /locus_tag="PLM90_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88766.1"
                     /translation="MKQRNTILTMLLIGSLALSSCYSTEVPETNKVTYIVTGTSFDVI
                     YQDPAGEYQYVYDREGSFKVYQESLVGESVFLLAKGSDDVVVHIRIKDGIDTVRKAKT
                     AGTSLVYIDYLPEIKE"
     gene            1883..3847
                     /locus_tag="PLM90_05045"
     CDS             1883..3847
                     /locus_tag="PLM90_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HMZ88767.1"
                     /translation="MQKFITAAFISVLAVTGWMATSNLREASPIEYNGTATISGIVTD
                     DVIGQPIIGAQIALNKTDWFTTDSLGQFTIDKLPQGAYSLLVKAEGYTDQEMSITLTE
                     GENKIVSISMSLATLISADTAITEFEQVYENENLKRKSVMANQESLTFGYATMDAPAI
                     SFRGDVYDEDVDGNYNTESYNAIQETGFKEAISNPLSTFSIDVDNASYSNIRRMINYG
                     QQIPADAVRIEEMVNYFDYTYAQPTNNDPFAVQYELGTCPWNSETSLVMIGIQGKDID
                     YSKTAASNLVFLIDVSGSMQSENKLPLVKSSLTKLIDNLKPTDRVALVVYAGAAGEVL
                     PSTPCSNSSKIKSALNQLEAGGSTAGGAGIQLAYEIATKNFIEGGNNRVILCTDGDFN
                     VGSSSDGEMTKLIEEKRKTGVFITVCGFGMGNYKDSKMESIADNGNGAYYYIDDKKEA
                     EKVFGTDLRGTLFTIAKDVKIQIEFNPVMVESYRLIGYENRILNKEDFDNDAKDAGEL
                     GAGHRVTALYEIKRRTTPSQPTGMQQNTIQEDLKYQSTTVKSTAYSTNELMTLKLRYK
                     APDGNTSKLIEKSLFNQPMAFAATSDNFRFASAVTEFGMILRNSEFKGDADINEVLQL
                     ASGAKGADKYGYRDEFIQLVKQYAGTTAKN"
     gene            complement(3932..4852)
                     /locus_tag="PLM90_05050"
     CDS             complement(3932..4852)
                     /locus_tag="PLM90_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HMZ88768.1"
                     /translation="MQHILITGGSGLLGRAITAQLHALGYRVAWMSRRIRPAGNSKDL
                     ALPDKVFLWNPESGHLDPEALSWSDVIIHLAGEGIADKRWTPSRKKIIIESRTKTTEL
                     LVSAMRALPQKKHLVIAASAIGFYGPSEKLLLESDPTGNGFLAYSVNEWEKATANFED
                     VALRAVTFRIGIVLSKNGGAYPQLLQTAMIRVLPFLGNGKQMYPWIHVDDVVDMVLFA
                     ISHPVSGTFNAVSPNPVTQAAIMNTYGDVTSGWYLKIPVPKFIIRLLLGEMADMVLLT
                     QMVSAEKIITAGYKFKFPDINAAIRDLEKK"
     gene            4983..6866
                     /locus_tag="PLM90_05055"
     CDS             4983..6866
                     /locus_tag="PLM90_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ88769.1"
                     /translation="MRRHLLPIACLISGIAFLSSCGGNKDSGAEIDVTEISDKIKCIK
                     LDDADSILPEWSKENILINHVVGNPDNFHPTNGAHAARSWILQYTSNFVLRSDIMNMD
                     IAPDLAVAMPEISANKLEYTYTLRKEARWDDGAPILAEDVVFMLKANKCPLTNNPITK
                     VYFDNIKNVITYPNDPYKFTIVMKREYILNISFLTDFPVMERKYHDPENVLAGYTFDQ
                     FNDPNFKADKDAKLTAWANNFNDPKWGNDLSLLNGSGPYKITEWSRDQALILERKKDH
                     WTQKLASPTVYDVSLPEKIIFKLVLDPNAQKLEFRSQTVDASVWMATPLVKELMTEAD
                     FNKNYNIEFTDNFSFNYIGLNMRPDGVLRKKFFDDVRVRRAMAYVVPVDEIIQAITFG
                     YAKRQASIVSPLKDEYNKDLTLIPLDIEAAKKLLDEAGWKDTDGDNVRDKVIDGVKTD
                     LRIEFKHQAGQKFVEDVARMIKESAYKAGIDLIVVPVDGITLKEQLGKHDFDMYMSAL
                     AGGSQAEDYTQLWHTSAYTSGGSNYCGFGSAETDALLDSIKYTVDPVARRPLMMRLQQ
                     EIYDQQPYIFLYSAARKNIIHKRFGNQIMTFDRPGVILNNLRLLSLYGPQGGSTLKDD
                     TMH"
     gene            6957..8459
                     /locus_tag="PLM90_05060"
     CDS             6957..8459
                     /locus_tag="PLM90_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88770.1"
                     /translation="MGRYLLKRILFFIPTLLIISLLAFIISLQAPGDPLDRMVSAQEN
                     GGEFQAQSANTQKEKDRWRKKLGLDLPVFYFSFSNAATPDTLYRIYDDNLRNASKRLI
                     NTYGNWEQISDWQKSLNILKNSLFEINALHAAAVDSSFSNADRDTIIEMDRLVSALLL
                     AYDTRLIEIKLHQLQATCSAHNYFSPASTSLAGTLNAWNNVKSQSTPGKKYIPAIHFY
                     GYNQYHRWIFGDGNWLTGKGAVDCKGWLRGDFGYSYETKLPVGNVLAERVRWSLFFTL
                     ISVTLAYLISLPIGIKAAAKKDSLFDRTSSVVLFVLYSMPAFWVATLLLMSFANPLAM
                     DIFPSSGIQPVTGIPEGTSWIEAVRMRLPYLVLPTIAYTYSQLAFLSRITRVSTLEII
                     SQDYIRTARAKGLSEGTVIYKHAFRNALLPIITVFSNIFPLAIGGSVILETIFTIPGM
                     GQQVFHAIVSKDYPVIITVFTLTGVLTLIGYLIADILYAIADPRISYANK"
     gene            8456..9784
                     /locus_tag="PLM90_05065"
     CDS             8456..9784
                     /locus_tag="PLM90_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88771.1"
                     /translation="MTKEKNDKGFNFGAYAFRQFRRNKPAYISLYILGFLALMALLAP
                     VIANERPLFISYKGHHLYPALSFKKNYILTNDDGTKETIQLDIADWKQMKADKVVWAP
                     IPYAPEKTDMLNAGYKGPHDAQLFKNSDGQIINMPSRFRHVMGTNSLGEDVASGLIHG
                     ARVSLTIGFIAMAIASVLGLILGSMAGYFGDSRLRTSRGQFWMVVLGIIAGWFYGFQT
                     RQFNLQDALSTGTGKLIMQLLLSISIAIAVTVIFALIGRFLGKLPFLNKQVNIPVDSI
                     VSRTIEIFHSMPTFILILTIAAIARPSMTNVMIIIGFTSWTGIARLTRAELLRIRQLE
                     YIQAARALGFGELKIILRHALPNGIAPALVSIAFGIASAVLIESSLSFLGIGVPSDVV
                     TWGSLVNDGRQQYSAWWLVVFPGVAIFLTVTVYNLIGEGLRDALDPKHKK"
     gene            9830..11332
                     /locus_tag="PLM90_05070"
     CDS             9830..11332
                     /locus_tag="PLM90_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88772.1"
                     /translation="MIIEGAMWRSAHVIWLIIIGLMLTGLPSKALAQDISISEPQKIS
                     TELIGYSILGRNKNGDVLIYKKYRFRDEIDILDKNMEFKRRKEFSVKNSDYETVEFYK
                     SGDYLLHFYTYRENKMSYLAVQQYNGDLDKKNEPVLIDSSSQRLGENFSEWKIAKAKT
                     TPYYLVYKYELSNGRIDKLFCRVMDESTRIIQQNDINLPDDQFSPILVKEAVSDDGIP
                     LFLFSNDDFNCKKEKTGVQYLYVFPKGNNSVVYTEISDENYCLDEMNFGLDNAHHSIV
                     SIGFLKEDNKDYMVGYLYSTIDILTGTKGTNFHYIFPQQTLDEIAGLAKEKAISNLPV
                     YQIGTIIPRVDGGALLVAEFYDKTVESYEFTNYDPYVGYRTSTRQVEFYEYDDILLFS
                     IKPDGTPSWNNVIRKKQISREDNGVNSSYGVLTLKDRCIFIFNEDVEQNSNVLQYEMS
                     FTGELDRKSLFNANQQEVQIRPGSAEQIALNEIIIPSIYKKSLRFVRIKF"
     gene            11372..12361
                     /locus_tag="PLM90_05075"
     CDS             11372..12361
                     /locus_tag="PLM90_05075"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HMZ88773.1"
                     /translation="MLRLFQSSNPFTFILLLVYTGLVFSHNLFFPAPITAASHGVLSD
                     GIWQLLRIDSWPLLAIELLQSILMLTTGILISFMMQKFKLINKTTLMPAAVFVLICSY
                     FPEMRIHIAETLCGLCLIILLYKIFGAYNKQRADMVYFDAAIISAIASLIYFPAVIFF
                     FFSLFGLLRMRSTSFREFIIYLSGWLTVAFLLGTYIFWNGSWEAWFASWEVQMHFISG
                     IGELMNATIIAKLSLIGLVFIVTLIFYFDRLSANLIQIRKYMGVFMLLFLFCLVTACL
                     GIGMDEGSLYLLWVPVTVVISYYFFHFKQLWYSEGMHAALLLGTFLFQYLTFA"
     gene            12374..13087
                     /gene="purQ"
                     /locus_tag="PLM90_05080"
     CDS             12374..13087
                     /gene="purQ"
                     /locus_tag="PLM90_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMZ88774.1"
                     /translation="MKFGVVTFPGSNCDQDIIHVLRNVMGQEVIELFHKEHDLRGLTT
                     ADCVMLPGGFSYGDALRTGAIARFSPIMQAVIDHCNKGGYAWGICNGFQILCEAGLLP
                     GVLLENNNQQFICKNVFLSADNNASRINATLPLQHALKIPIAHGEGRYYAEARELEAL
                     EANNQVIFRYCDDHGHITERANPNGSLNNIAGVCNAGRNVFGMMPHPERAAEERLGNT
                     DGRLIFESFLALTEATAAR"
     gene            13188..15605
                     /locus_tag="PLM90_05085"
     CDS             13188..15605
                     /locus_tag="PLM90_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMZ88775.1"
                     /translation="MKRLLIALCSLFLFTNVKAQILEPVKWEVSSTQTGVNEYVLIFK
                     ATVQEGWKIYSHSVPENGPIPTSLIFENLPAGVQLVGEMEELGRMEEQQEPLFDNNII
                     KFYHKRLTLRQAVKISGNAKITGYIDYMSCNDQTCTALSYDFSFDLEGQSGFVQVPGS
                     DYANVKYPVTWKIGSEKLNDNEYYITYSATIVPTWKLYAQTSPEGAARPMKFIFENDG
                     QGYTRIGGVEEIGHMEEKKEQLFENKTVRFYHDEVMMRQRIRIDSANANIIGYIDYQT
                     CDATQCKFMQKDFNLALSATGLIETGSTTPIDTGGMKFGAFAVDLKYAENAMCEGEVN
                     AQITNATGKGLFSTFILGFIGGLIGLLMPCTFPMIPFTISFFTKHAGNRRKGVMNAAF
                     YGLCIVLVYFIASLPFLLFGAGGDQLNEFSTNNIVNLVFFFVFILFALSLFGLFEIKL
                     PSKWANKADSASDVGGLLGIFFMALTLCIVSFSCTGPILGSLLANSIKDGGVALTMGM
                     LGFGTAFALAFTLLALFPQLLGNLPKSGGWLNEVKIVFAFIELAMAVKFLSNVDLAYH
                     WGYVKREIFLGIWTLIALLIVLYISGVIRFPHDDKKVKWSLGRVSFLITFLFFTIYFG
                     QGLRKYGEVGLLSGFLPPQWYSIYEYGTECPLGLNCFHDLDEGIAFAKEHNKPILVDF
                     TGYACVNCRKMEENVWPEEEVFNIINNDYVLVSLYVDDKEALRLEDQYISAATGQKVR
                     TIGNKWSDFQSSQFNTNSQPYYVLLAPDGKMLNQPRAYTPNVSEYRGFLECGIETFKN
                     MDKIAAK"
     gene            15744..16121
                     /locus_tag="PLM90_05090"
     CDS             15744..16121
                     /locus_tag="PLM90_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ88776.1"
                     /translation="MHENDISYKVRGAIFTVFDSLGQGLPESAYEAALSHKLIIAGLN
                     VRRQVSLPLVYDGVKMDIGYRIDLLIEDLVIIEIKSAENLTKVHHKQLLTYLKLSGHK
                     LGILVNFNCSDITKNIFRKVNYL"
     gene            complement(16412..18238)
                     /locus_tag="PLM90_05095"
     CDS             complement(16412..18238)
                     /locus_tag="PLM90_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HMZ88777.1"
                     /translation="MRKLLTLSLLIPIALISCKPKEKAEDNSAIQKEVQTYLDGYNAE
                     YQKLVTASSEGQWILQTHIVEGDTMASHNAAIADEAYAKFTGSKTNIETAQKYLAMKD
                     KLTAIQVRQLEYIRYLAGNNPETAGDVVKNRITATNAQVEKLFGFKYTLNGKPVTTNQ
                     LDEILRSSNNMNERLAAWTASKEVGVGLKDGLVELQKLRNESVTPLGYSDFFAYLASE
                     YNMTSEEMLELTRSFITDVWPLYRELHTWARYELAEKYNQPVPDYIPAQWLPNRWGQD
                     WTAMVNVEGLDIDPELKKHDAEWVMKQGEAFWMSLGFDALPQSFWEKSSLYPVSADAG
                     YAKNNHASAWHIDLDKDVRSLMSVEANTEWWSTVLHELGHIYYYMSYSNPDVPPVLRT
                     GANRGYHEAFGTMIGLASLQKPLLENQGLIKPGVVTNDTLKLLSEALDYIVHIPWGSG
                     VMTEFEYALYAKNLSPDQYNAKWWELVKQYQGIVPPSPRDEHYCDAATKTHINDDPAQ
                     YYDYSISNVLLFQFHDYIAKNILHQDPHATNYWGSKETGDFLRKVMTPGATVDWREHL
                     KASIGSDMSAKPMLDYFMPLMDYLKKQNAGRTYTLPEKPTFN"
     gene            18441..19454
                     /gene="recA"
                     /locus_tag="PLM90_05100"
     CDS             18441..19454
                     /gene="recA"
                     /locus_tag="PLM90_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMZ88778.1"
                     /translation="MAEATNDKVKALGLAVDSLNKLYGKGTVMKLGESAVTAEEVIPT
                     GSLGVDMALGIGGIPKGRVVEIYGPESSGKTTLAIHVIAEAQKKGGICAIIDAEHAFD
                     KVYAEKLGVDVENLLISQPDYGEQALEIADHLIRSGALDVVVIDSVAALVPKGELEGE
                     MGDSKMGLQARLMSQALRKLTGTINKTGCCCIFINQLREKIGVMFGNPETTTGGNALK
                     FYASVRIDIRRIGQLKDKDDVIGNRVKVKIVKNKVAPPFRQAEFDIIYGEGISKIGEI
                     IDLGAESGVIQKSGSWYSYEGTKLGQGREGVKDILRDNPELALEIEKKIKAKLSGVPT
                     KED"
     assembly_gap    19683..19782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20045..20641
                     /locus_tag="PLM90_05105"
     CDS             20045..20641
                     /locus_tag="PLM90_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88779.1"
                     /translation="MTTFKTRDEQDVIWFITFTCYRWLPLIETTDLYDDIYKWFDILK
                     SEGHSILGYVIMPNHMHMLMYFSKNKRTINTCMAEGKKFRAWEIIKRLKKQRASHYLI
                     QMEWVPEDHKNPNKQKFMAFEPSFDCKVCKSEERVLTKLNYIHKNPVNKGYHLAPSMT
                     AYKHSSAKYYHTGIHSAYPVEHYKRFIRPFNCNIDAPQ"
     gene            20740..22233
                     /locus_tag="PLM90_05110"
     CDS             20740..22233
                     /locus_tag="PLM90_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88780.1"
                     /translation="MNIKANCYTLLIALLTRIPAFGQLEAVDTISYPVDSEHSTYVFQ
                     NLGNDGYILMYKSNSDRVELSKYDTLNNETNHNSFEVVAHLRYAVYPDKSYSDYFDIS
                     ENNEVVNHRIDFKTLDVVTTKCSVDIKLKYKYQVSNNDCILLVFENYFKQEIYKVDIK
                     TGQVNRIISDLPEKKKKSFAYQQITVVPSTGEFFLFTNKQKFFKTYNLSAHLNNEGEQ
                     IGAYVLNPKPSKFYQNYTVVATPDNEYQIWCEMKYIKKLSDYGNPKISGLCQIVIDDE
                     SVVSRKSIPFRSFKNFYAFQKDNKSKNIDKNSLKYRMGHPTKVNMHTIQIKLVDDAVY
                     YAGEVYWPVYKTVVEQTVGSTAKPTKKMVLDYVIYSHAVIAKFDLEGNMIWNNSVELD
                     VDDTEAVLQINKYQINKNGVGVLLINEGAITLTKFDADGRIVSKYNTPCSAPICVDEE
                     KRKFNFFSLQYWYNDYMVYPEYTIDDDPSTERTEGYLNLYKYELIQD"
     gene            complement(22408..>23058)
                     /locus_tag="PLM90_05115"
     CDS             complement(22408..>23058)
                     /locus_tag="PLM90_05115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ88781.1"
                     /translation="DEIIAEGLVKVNGVIVTEMGYRLKPDDKVTYKGKAVKPVNYVYV
                     LLNKPKDYITTVDDDRGRHTVMELVAKATTERIYPVGRLDRNTTGLLLLTNDGELAQK
                     LMHPKYGAQKLYEVELDKQLSQTDMQKIADGVELEDGVAYVDGIDYAHVAKKNVIGIS
                     IHIGKNRIVRRIFEHLGYEVVRLDRTLYAGLTKRDLPRGRWRHLTEREVVKLKYLK"
BASE COUNT         6895 a         4495 c         4852 g         6716 t
ORIGIN      
        1 agtcaagggt aaatccatgg aatattacac acgcacctcc ctgcattcaa tcggctaatt
       61 ggctaatcgg ctaattggct aattatttgc acatagagcg cagcgacatc ccgccctcag
      121 cgggacttcc aatatttcac actctcactt gcacacttgc acacttgcac actcgcacac
      181 tagtacactc gcacactcta tacttctatc tttgcaaaaa tcttttctca tgcacaatcg
      241 cattacagca ttattcggaa tagagtatcc tatcattcag gcgggaatga tttggtgttc
      301 ggggtggaaa ttagcgagtg ctgtttctaa tgccggtggg ttaggattga taggtagcgg
      361 gagcatgtac cctgatgtat tgcgtgaaca tattcaaaaa tgtaaggccg ctaccaacaa
      421 gccttttgga gtaaatgtac cgctcttata tcctgatatt gacaagcaca ttcaaataat
      481 tattgaggag ggtgtgaaaa tcgtttttac cagcgccggg aatccaaaga catggacttc
      541 catgctcaag caacatggca ttaaagtagt gcatgtagtg agcagtcgaa agtttgccct
      601 aaaatgtcag gaggcaggat gcgatgcggt tgttgccgaa gggtttgagg cgggtggtca
      661 taacggtcgc gaagaaacca ctactttatg cctgattcct agtgttgccg aagctgtcac
      721 aattccggta attgctgcag gtggaattgg cgatggcagg gctatgctgg cgtgcatggt
      781 tttaggagca gaaggggtgc aaattggcac gcgatttatc gccagtatcg agagcagtgc
      841 gcatgacgca ttcaagcaaa ccgttattca tgccgatgaa ggcagcacca tactcacact
      901 gaaggaaata accccggtta gaatggttcg aaatgctttc tatgacatgc tttcagcagc
      961 ttatgcccgt tgtgcaagtg ccgacgaact caggacaatt ctagggcgcg gcagggctaa
     1021 aaaaggtatg tttgagggcg atatggaagc aggtgaacta gaaataggac aggttagcgc
     1081 tacggtacga gaaatattgc ccgcagctga aatcctgaag gacatttggg agggttattt
     1141 ggtggcaaaa tcaggcctga ctaagcaatg aaccatgccc aaaagcaagg taacctaact
     1201 tcaatagtga cgatatcgat gtcacattta aaccgttttt tatacatttt tttgtcagca
     1261 ataaggtgct ctagacgata ttttcatact tttgcctcgt tttaccatgt cacacaataa
     1321 catttactgt atatacatac gccctgtgga aatgtctatc ttagtatttc aagtatcacg
     1381 agtatgaagc aacgcaacac aattttaaca atgttactga ttggttctct ggcactttcg
     1441 tcgtgctaca gtaccgaggt gcccgaaacc aataaggtca cctatattgt aacaggaaca
     1501 tcttttgatg ttatctatca agaccctgcg ggagagtacc aatatgttta cgaccgagaa
     1561 ggatccttta aagtttatca ggaatcttta gttggcgagt cggtatttct gcttgcaaag
     1621 ggcagtgatg atgtggtagt gcatatccgt attaaagatg gcattgacac agttagaaag
     1681 gcaaaaacgg cagggacttc attggtctat attgactatt tgcctgaaat aaaagaataa
     1741 acgctttcac accttatttg aacgtcccgg ttttgcgacc gggatttttt tttgtcccaa
     1801 cgattattga cggcttttat ggaaaagaga attttcatgc atcaggatag cagttattac
     1861 ttcactttaa aaatctgcta tcatgcaaaa attcatcacc gcggccttta tatctgttct
     1921 tgcagttaca ggatggatgg caacctcaaa ccttcgcgaa gcatcgccga ttgaatataa
     1981 tggcacagct acaatttcag gcatagtgac cgatgatgtt atcgggcaac caattattgg
     2041 cgcacagatc gcattgaata aaacggattg gtttactact gattcacttg gacaatttac
     2101 tattgacaaa cttcctcaag gggcatatag tttgttggta aaagctgaag gttataccga
     2161 ccaggaaatg tcgataacac ttactgaagg cgaaaataaa attgtcagta tcagcatgtc
     2221 tcttgcaaca ttgataagtg ctgatacagc cattaccgag tttgaacagg tttatgaaaa
     2281 tgaaaattta aaaagaaaaa gtgtgatggc caatcaggag tctctgacat ttggttatgc
     2341 cacaatggat gctccggcaa tttcattcag aggtgatgta tatgatgaag atgttgacgg
     2401 aaattacaac acagaatctt acaatgccat acaagaaaca ggttttaagg aggctatcag
     2461 caatccactt tcaacatttt ctattgatgt agataatgcg tcgtattcaa acattcgcag
     2521 gatgattaat tatgggcaac aaattccggc tgatgctgtg cgcattgaag aaatggtaaa
     2581 ttattttgac tacacctatg cacaacctac aaacaacgac ccgtttgcag tgcaatatga
     2641 attgggaaca tgcccctgga attcggaaac atcgctggtg atgataggca tacaaggcaa
     2701 agacatcgat tattctaaaa ctgccgcttc aaaccttgta tttctgatcg atgtaagtgg
     2761 tagcatgcag agcgaaaata aattgccctt agtaaaaagc agtttaacca agctgattga
     2821 caatctaaaa ccaacagatc gcgtagcctt ggtagtatat gccggtgcag cgggtgaagt
     2881 attgccatcg acaccctgca gcaatagttc taaaatcaaa agcgccctca atcaattaga
     2941 agcaggaggc agcactgcag gcggagcagg tattcagctg gcatatgaaa ttgccacaaa
     3001 aaattttatt gaaggtggca acaaccgagt gatactttgc accgacggcg atttcaatgt
     3061 aggctccagc agcgatggag agatgactaa gcttatcgag gaaaaaagaa aaacaggtgt
     3121 atttattacc gtttgcggct ttggaatggg aaattataaa gacagtaaaa tggaatcgat
     3181 agcagataat ggcaatggtg catattatta catcgacgac aaaaaagaag ctgaaaaagt
     3241 ttttggaaca gatcttcgag gtacgttatt taccatagct aaagatgtaa aaattcaaat
     3301 agaattcaat ccggttatgg tagaaagtta tcgccttata ggttatgaaa acaggatttt
     3361 gaataaagaa gatttcgaca acgatgcaaa agatgccggt gaattgggtg caggacatcg
     3421 cgttacagca ctgtatgaaa tcaaacgtcg cacaacgcca tcacaaccaa caggaatgca
     3481 acaaaatacc atacaggaag atttgaaata tcaatcgaca actgttaaat ccactgccta
     3541 ttctaccaat gaattaatga cattgaagct gaggtataaa gcccctgatg gaaataccag
     3601 caaactcatt gaaaaatcac ttttcaatca acccatggca tttgcagcta ccagcgataa
     3661 tttcaggttt gcaagcgcag taacagaatt tggtatgata ctgagaaatt cagaattcaa
     3721 aggcgatgct gatataaatg aagttttgca attggcaagt ggcgctaagg gtgcagataa
     3781 atacggctat cgcgacgaat ttattcaact ggtaaaacaa tatgcaggaa caactgccaa
     3841 aaattaaaat tcgaaaaaaa gttagggcgt cttttaataa atcttgatgt ttgatgcaat
     3901 tccggggtat ggcgaaagct gtattccgga attatttttt ttcaaggtca cggatagctg
     3961 cattgatatc cggaaattta aatttgtaac ctgctgttat tattttttct gccgacacca
     4021 tttgtgtgag cagtaccata tcagccattt ctcccaacaa taatcgaata ataaatttcg
     4081 gaacaggaat tttaagatac catccggatg ttacatcacc atacgtattc atgattgccg
     4141 cctgggtgac aggatttgga gatactgcgt tgaaggttcc tgaaacagga tgacttatgg
     4201 caaaaagcac catgtccacg acatcatcca catgtatcca agggtacatt tgttttccat
     4261 tacccaaaaa aggcaacacc cttatcattg ctgtttgcaa caactgcggg taggcaccac
     4321 cgttcttcga caatacgata ccaattctaa acgtcaccgc tcttaaggca acatcctcaa
     4381 aatttgccgt agccttttcc cattcattca cgctgtatgc caaaaatcca ttgccggtag
     4441 gatcagattc gagaagcagc ttttccgatg gtccgtaaaa accgatggct gaagcggcaa
     4501 taaccagatg ttttttctga ggcaacgcgc gcatggccga caccaacagt tctgttgttt
     4561 ttgtgcggct ttcaatgatg attttttttc tggagggtgt ccatcgctta tctgcaatac
     4621 cctctcctgc caagtggatg atgacatcac tccatgatag cgcctccggg tcgagatgcc
     4681 cggattccgg gttccacagg aaaactttat ccggcagcgc aaggtctttc gagttgcctg
     4741 caggtcttat cctcctactc atccacgcaa cgcgatagcc caatgcatgc aattgtgctg
     4801 taattgccct tcctagtagc ccgctaccgc cggtgatgag aatgtgttgc atttagtgga
     4861 gttaaacagt agagtgccga attggtttac cttttgcagc aattttcgtt ccaaaatcag
     4921 atatcaggaa atcgccgaat cgcataaaat tcttactttc gggtcgtaat taacaatcta
     4981 tcatgcgtag acatttactg ccaattgcct gtcttatatc aggcattgca tttttgagtt
     5041 cttgtggagg aaataaggac tccggtgccg aaattgatgt tacagaaata tcggataaaa
     5101 tcaagtgcat caaattagat gatgctgatt ccattttacc ggaatggagc aaagaaaata
     5161 ttcttatcaa tcatgttgta ggcaatcccg acaatttcca tccgacaaat ggcgctcatg
     5221 cagcaagaag ctggatactt caatacacca gcaattttgt attgcgcagc gacattatga
     5281 atatggatat tgctccggat ctggcagttg ccatgcctga aatatctgcc aacaaactcg
     5341 aatacactta caccttacgt aaagaagcgc gatgggatga tggtgctccg atcttagcag
     5401 aggatgttgt ttttatgttg aaggcaaata aatgtccgct tacaaataat ccgatcacaa
     5461 aagtttattt cgacaatatt aaaaatgtga tcacttatcc aaacgatccc tataagttca
     5521 ccatcgtgat gaagagagaa tatattttaa atatatcttt tcttaccgat ttcccggtga
     5581 tggagcgcaa gtatcacgat cctgagaatg tgcttgctgg ttatacattc gaccaattca
     5641 atgatccgaa tttcaaagcc gacaaggatg cgaaacttac agcgtgggca aataatttca
     5701 atgatccgaa atggggtaat gatctttctc tgttaaatgg ttcaggacct tacaaaatta
     5761 ctgaatggtc gcgcgatcag gctttgatcc tcgagcggaa aaaagaccat tggacgcaaa
     5821 aattagcatc accaacagta tatgatgtat cacttcccga aaaaatcatt ttcaaactcg
     5881 tacttgaccc gaatgcacaa aaacttgaat tccggagtca aacagttgat gcttctgtat
     5941 ggatggctac accattggta aaagaattaa tgacggaggc agatttcaat aaaaattata
     6001 atatagaatt taccgacaat ttcagtttta attatatcgg attgaacatg cgtcccgatg
     6061 gtgttttgcg aaaaaaattc tttgatgatg tgcgcgtgcg tcgtgcaatg gcatatgttg
     6121 tacctgtaga cgagatcata caggcgataa cctttggata tgcgaaacgt caggcaagca
     6181 ttgtatcacc actgaaagat gaatacaata aagacctcac attgattccg ctcgacatag
     6241 aagcagcaaa aaaattactt gatgaagccg gatggaaaga tactgatggc gataatgtgc
     6301 gcgacaaggt aatagatggc gtgaagacag atttgcgtat tgaatttaaa catcaggcag
     6361 gacaaaaatt tgttgaagat gttgcgagga tgatcaaaga atcagcatat aaagccggta
     6421 ttgatcttat tgttgttcct gttgatggca ttacactaaa ggaacagcta ggcaagcatg
     6481 attttgatat gtatatgagt gctcttgccg gtggatcgca ggcagaggat tatacacaat
     6541 tatggcatac cagcgcttat acttccggcg gatcgaatta ctgcggattt ggttcagcag
     6601 aaaccgatgc actgcttgat tccatcaagt atacggtaga ccctgttgcc cgcaggccgc
     6661 tgatgatgcg acttcaacaa gagatctatg accagcagcc ctatattttc ttgtattctg
     6721 ctgccagaaa aaacatcata cataaacgtt tcggaaatca aatcatgacc tttgatcgcc
     6781 cgggtgtgat actaaataac ttgcgtttat tgagtttata tggccctcag ggaggatcta
     6841 ccctgaaaga cgatacgatg cattaacaac atttcactac cttacaaggg gtgtgcggcg
     6901 ataattaagg ctgcacaccc ttttcttttt ttatcttagc gtgcaattaa tctacaatgg
     6961 gcaggtatct tttgaagcgc atattatttt ttattcctac cctgctgatc atttcacttt
     7021 tagcattcat cattagtttg caggcaccgg gagatcctct ggacagaatg gttagtgccc
     7081 aggaaaatgg aggagagttt caggcgcaat cggcaaacac tcaaaaagaa aaagaccgtt
     7141 ggcggaaaaa attaggatta gaccttcctg tattttattt cagcttcagt aatgccgcaa
     7201 ctcctgatac actttatcgc atttatgatg acaatttgcg caacgcttca aagcgtctga
     7261 tcaacacata cggaaattgg gaacaaatat ctgattggca aaaaagcctg aatattttaa
     7321 aaaattcact ttttgaaata aacgcacttc atgccgctgc agtggacagc agtttttcca
     7381 atgccgacag agatactatc attgagatgg acaggctggt tagtgcattg ttattagctt
     7441 acgacacgcg gttgatcgaa attaaattac atcaattaca ggcaacctgt tcggcacaca
     7501 actatttttc tcctgcatca acctcactag caggtacact aaatgcctgg aataatgtaa
     7561 agtcgcaatc cacccccgga aaaaaatata tacctgccat tcatttctat ggttataatc
     7621 aatatcacag atggattttt ggcgatggca attggcttac gggaaaaggt gctgttgatt
     7681 gtaagggatg gcttcgcgga gattttggat atagttacga aactaaattg cccgtaggaa
     7741 atgtgttggc agaacgcgta aggtggtcgt tgttttttac tttaatttca gtaacacttg
     7801 cgtatttgat cagcttacct atcggaatta aagctgcagc gaaaaaagac tccctgtttg
     7861 acagaacttc atcggtagtt ttatttgttt tatatagtat gccggcgttt tgggtagcta
     7921 ctttattatt gatgtcgttt gccaatccat tggcgatgga tattttcccc tcgagtggca
     7981 ttcaaccggt aaccggaatc cccgaaggca cctcatggat agaggctgtg cgtatgcgat
     8041 tgccatatct cgtattaccg accattgcat acacttattc tcaacttgcg ttcttaagtc
     8101 gcattacaag agtgtcgact ttggaaataa tcagtcagga ttatatccgc accgccagag
     8161 ccaaaggatt atcggaaggc actgtgatat acaagcatgc attcagaaat gcattattgc
     8221 cgattattac ggtgttcagt aacatttttc cacttgccat cggtgggtct gtaatactgg
     8281 aaaccatatt taccattccc ggtatgggcc agcaggtgtt tcatgctatt gtgtcgaaag
     8341 attatccggt aatcataact gtatttacat taacaggtgt attaacacta ataggttatc
     8401 tgattgccga tattttatac gccattgccg atccgcgaat ttcatatgct aataaatgac
     8461 gaaagagaaa aacgataagg gatttaattt cggcgcatat gcgtttcgtc aattcaggcg
     8521 caataagcct gcatatatat cattatatat acttgggttt cttgctttaa tggcattgct
     8581 ggcgccggtt atcgccaacg aacgtccatt atttatcagc tataaaggac atcatttata
     8641 tccggcgtta agtttcaaaa aaaattatat cctgactaat gatgatggca cgaaggaaac
     8701 cattcaactg gatatagccg actggaaaca aatgaaggcg gataaggttg tatgggcacc
     8761 cattccttat gcacctgaga aaacagacat gcttaatgcc gggtataaag gtccgcatga
     8821 tgcgcaatta tttaaaaatt cggacggtca gatcattaat atgccatcaa gattcaggca
     8881 tgtaatggga acgaatagtt taggggaaga tgtggcgtca ggtttgatac acggtgcaag
     8941 ggtatcgctg actattggct ttattgcaat ggccattgcg tcagttctcg ggttgatatt
     9001 aggctcaatg gcaggctatt tcggcgatag cagattgcgc acttcaagag gccaattctg
     9061 gatggtcgta ttaggaataa tagcaggttg gttttacgga tttcaaacgc gacaatttaa
     9121 tttacaggat gcccttagta caggaacagg aaaattaatc atgcaactac tgcttagcat
     9181 tagtattgca attgctgtga cagtaatatt tgcattgatt ggacgatttt tggggaaatt
     9241 acctttcctc aacaagcagg taaacattcc tgttgactcc attgtatcac gaacgataga
     9301 gatatttcac tctatgccaa cgtttatttt aattttaact attgcggcaa ttgcacgtcc
     9361 ctcgatgaca aatgtgatga tcattatcgg attcacttca tggacaggta tagccaggtt
     9421 aacacgcgct gaattgttgc ggatacggca attagaatat attcaggcag cacgggcact
     9481 gggattcggt gaattaaaga tcatattgcg ccatgcgctt ccaaatggca ttgccccggc
     9541 actggtttca attgccttcg gcatagcatc ggctgtatta attgaatcaa gtttgtcatt
     9601 tttaggtatt ggcgttccct cggatgtggt aacctgggga agtctggtaa atgatgggag
     9661 gcaacagtat agcgcctggt ggttggttgt gttcccggga gttgctatat tccttaccgt
     9721 aacggtgtat aatctcattg gtgaaggact tcgcgatgct ctggatccga aacataaaaa
     9781 ataatcccgg ctacactgcc gttgttttaa tcagcttact ttatttttga tgattattga
     9841 aggtgctatg tggcggagtg cacatgtaat atggctgatt atcattggtt taatgcttac
     9901 gggattgccg tctaaagcat tggcacaaga catctctata tcggaaccgc aaaaaatcag
     9961 caccgagtta attggttaca gtattttagg tcgcaacaag aatggcgatg tgcttattta
    10021 taagaaatat cgtttcaggg atgaaataga tattttggac aagaatatgg agttcaagcg
    10081 tcggaaggaa ttcagtgtta aaaattctga ttacgagacc gttgagttct ataaatcggg
    10141 cgactatctc ttacattttt acacttatcg cgagaataaa atgagttatc tcgctgttca
    10201 acaatataac ggcgacttag ataaaaaaaa tgaacctgta ttgatcgact cctcttcaca
    10261 gcgtttaggt gaaaatttct cggaatggaa aattgccaag gccaaaacaa ccccctatta
    10321 tctggtgtat aaatatgaat tgagcaatgg ccgcatagac aaactgttct gccgtgtaat
    10381 ggacgaaagc acacgcatca tacagcaaaa tgacatcaac ctgcctgatg atcaattcag
    10441 ccctatttta gtaaaagaag cagttagcga tgacgggata cctttatttc tattttcgaa
    10501 tgatgacttc aattgcaaga aagagaaaac aggagttcaa tatttatacg tattcccaaa
    10561 aggaaataac agcgttgttt atacagagat ctctgacgaa aattactgtt tggatgaaat
    10621 gaatttcggg cttgataatg ctcatcacag cattgtgagc atagggtttc ttaaagaaga
    10681 taataaagat tatatggttg gatatttata ttccaccatt gatatactaa ccggcacaaa
    10741 aggaacaaat tttcattaca tatttcctca acaaacactt gatgagattg cgggcttagc
    10801 aaaggaaaaa gcaatcagca acttaccggt gtatcaaatc ggcaccatta ttcctcgtgt
    10861 tgacggaggc gcattgctgg ttgctgaatt ttacgataaa accgttgaaa gttatgagtt
    10921 caccaactac gacccttatg ttggatacag gacatccaca cgtcaggtcg aattttatga
    10981 atatgacgat attttattgt tctccataaa gcctgatggc actccttcct ggaataatgt
    11041 gattcgtaaa aaacaaatca gcagagaaga caatggcgtg aattcatcct atggggtttt
    11101 aacactgaaa gacaggtgta ttttcatatt caatgaagat gttgaacaaa attcgaatgt
    11161 attgcaatac gagatgtcat tcaccggtga gctagaccga aagagcttgt tcaacgccaa
    11221 tcaacaggag gttcagataa ggccgggaag tgccgagcag attgctttga atgagattat
    11281 tatccccagt atttataaga aaagtctgag atttgtgcgc atcaaattct aggatgcagt
    11341 aatgtttaat ttggaagtgt aattttacat cgtgctgcgt ttatttcaat cgtcaaatcc
    11401 atttaccttt atactcctcc tcgtatatac gggtttggtg ttttcacaca atttattttt
    11461 ccctgcgcct attactgcag cttcgcatgg ggtgttgagt gatgggatat ggcagttgtt
    11521 acggattgat tcatggccat tgcttgccat tgaattatta caatcgatat tgatgcttac
    11581 cacagggata ttaatcagtt ttatgatgca gaaattcaag ctcatcaata aaaccacctt
    11641 gatgcccgct gctgtttttg tgttgatatg cagctatttc cctgagatgc gcatacatat
    11701 tgcagaaaca ctgtgtggat tgtgtttgat cattttacta tataagatct ttggcgccta
    11761 caacaaacag agagctgata tggtgtattt cgatgcagcc atcatcagtg caattgcctc
    11821 gttgatctat ttccctgctg taattttctt ttttttcagt ctttttggat tgctgcgaat
    11881 gcgctcgacc tcattcagag aatttatcat ttacttatca ggatggctaa cggttgcatt
    11941 tttattggga acatatattt tctggaatgg cagttgggaa gcctggtttg cctcctggga
    12001 ggtacaaatg cactttattt caggcattgg agaacttatg aatgcaacca taatagctaa
    12061 gttgagtttg atagggttgg ttttcatcgt caccctaatt ttttatttcg accgcctatc
    12121 ggcaaacctg atccagatcc gcaagtatat gggggtattc atgttgttgt ttttattttg
    12181 cctggtaact gcctgccttg gaattgggat ggatgaagga agtttatatc tgttgtgggt
    12241 tccggttacg gtggttatca gctattattt cttccatttt aagcagttat ggtattcaga
    12301 agggatgcat gctgctcttt tattaggtac attcctcttt cagtacctta cctttgcata
    12361 acaatcagga atcatgaaat ttggcgttgt aacctttccc ggatctaatt gcgatcagga
    12421 tatcattcac gtattacgca atgtaatggg tcaggaggtg atagagcttt tccataaaga
    12481 gcatgatctg cgtggactta ccaccgccga ctgcgtgatg cttcccggag ggttttccta
    12541 tggcgatgcg ctcagaaccg gcgccatagc acgattttcg cctatcatgc aagccgttat
    12601 tgaccattgt aacaagggcg gatatgcctg gggaatatgc aatgggtttc aaattctttg
    12661 cgaagccgga ttgttgccgg gcgtgctttt ggagaataat aatcagcagt ttatctgtaa
    12721 gaatgtcttt ttaagtgccg ataataatgc cagcaggatc aatgcaacgc tgccactgca
    12781 acatgcactc aagataccaa ttgcccatgg cgaaggccgt tactatgctg aagctcgtga
    12841 acttgaggca ctggaagcca acaatcaggt aattttcagg tattgcgacg accatggtca
    12901 tataaccgaa agggctaatc cgaatggctc gctgaataat atagccggtg tttgcaatgc
    12961 ggggcgcaat gtatttggca tgatgcctca cccggaacgc gctgctgaag agcgtttggg
    13021 caataccgac ggccgcctaa tcttcgaatc attccttgca ttaaccgagg ccacagcagc
    13081 aagataacag cacctaagcc ggtttctcgt aatatttttg tcaatgccta cccaacagaa
    13141 cctgtagggg cagcttttcg ttaaatttgc atcactaaag ccgaaagatg aagaggttac
    13201 ttattgcatt atgctccctg tttttgttta cgaacgttaa agcccaaatt ctggaacccg
    13261 ttaaatggga ggtctccagc acgcaaacag gagtaaacga gtatgtgctg atcttcaaag
    13321 cgacagttca ggaaggatgg aaaatttata gtcattctgt tccggaaaac ggaccaatac
    13381 ctacaagtct tattttcgaa aatcttcctg caggtgttca acttgttggc gaaatggagg
    13441 aactggggcg tatggaagaa cagcaggagc cacttttcga taataatatc attaaattct
    13501 atcacaaacg tttgaccttg cgtcaggcag tgaaaatctc aggtaatgca aaaattaccg
    13561 gatacatcga ttatatgtcg tgcaacgatc agacatgcac agcgctttct tatgatttta
    13621 gcttcgacct ggaaggacaa agtggttttg ttcaagtgcc gggatctgac tacgccaatg
    13681 taaaatatcc ggtaacatgg aaaattggca gcgaaaaatt aaacgataac gaatattata
    13741 ttacctattc tgcaaccata gttcctactt ggaaattgta tgcacaaact tctccggaag
    13801 gagcagcacg accaatgaaa ttcatttttg aaaatgatgg acagggatac accagaattg
    13861 gtggtgttga agagatcggt catatggagg aaaagaagga gcaattattt gagaacaaaa
    13921 ccgttcgctt ttatcacgat gaagtaatga tgcgtcaacg tatcagaata gattcggcaa
    13981 atgcaaatat tattgggtat atcgattatc aaacatgcga tgccacacaa tgtaaattca
    14041 tgcaaaagga tttcaatctg gcattatcgg caaccgggtt aatcgaaaca ggctccacta
    14101 ctccgataga tacgggagga atgaaatttg gcgcctttgc agtagatcta aagtatgctg
    14161 aaaatgcaat gtgcgaaggc gaagtaaatg cacagatcac caatgcaacc ggcaaaggat
    14221 tgttcagcac atttatactc ggtttcattg gtggattaat tggcctttta atgccttgta
    14281 ctttcccgat gatcccattt acaatttcat ttttcaccaa acacgcaggc aatcgcagga
    14341 agggtgtgat gaatgcagca ttttatggat tgtgtatcgt gctggtatat ttcattgcct
    14401 cactgccatt tcttttattc ggcgccggtg gcgaccagtt gaatgaattt tcgacaaaca
    14461 atattgtcaa ccttgttttc ttctttgttt ttattctatt tgccttatca ctttttggtt
    14521 tatttgaaat aaagcttcca agcaaatggg ctaacaaggc tgatagtgcc tcagatgtcg
    14581 gaggcttact cggtatattc tttatggcac ttacactgtg cattgtgtca ttcagctgca
    14641 caggtccgat cttaggatca ttgcttgcta acagcattaa agatggtggt gttgcattaa
    14701 caatgggtat gcttggtttt ggaacagcat ttgcattggc atttacatta ttggcattat
    14761 tcccacaatt attaggtaat cttccgaaaa gtggcggctg gttaaatgaa gtgaaaatcg
    14821 tgttcgcatt tattgaatta gcaatggctg tgaaatttct aagtaatgtg gaccttgctt
    14881 atcactgggg atatgtaaaa cgtgaaattt tcctcggcat ttggacttta attgccctgt
    14941 tgattgtatt atatatcagc ggagtgatca gatttccaca cgatgataaa aaagttaaat
    15001 ggtcgctggg aagagtttca tttttgataa catttttatt ttttaccata tactttggac
    15061 aaggacttcg caaatacggt gaggttggtt tgttgagtgg atttcttcca ccgcaatggt
    15121 atagtattta tgaatatggc actgaatgcc ctcttggatt aaactgcttt catgatcttg
    15181 atgaagggat agcttttgca aaggagcata ataaacccat tttagtagac ttcaccggtt
    15241 atgcatgcgt aaattgcaga aagatggaag agaatgtttg gcctgaagaa gaagtgttta
    15301 acattatcaa taatgactat gtattagtga gcttatatgt tgatgacaaa gaagcattgc
    15361 gattagaaga tcaatatata tctgccgcta caggtcaaaa ggtacgcacc attggtaaca
    15421 agtggagtga ttttcaaagc agtcagttca atactaactc acagccatac tatgtattgt
    15481 tggcacctga cggaaaaatg ctgaatcagc ctcgtgcata tactccgaat gtctcagaat
    15541 atcgtggatt tttagagtgt ggtattgaga cattcaagaa catggataaa attgctgcga
    15601 agtaattaag cccttagcat cacgggcctc tatactcaaa atcaaattaa tattttttat
    15661 tgcgaattgt cgttcagata attcgcattt ttttttattt cgtgcacgct aaatagaaaa
    15721 atcatataac attggggcta tctatgcatg aaaatgatat ctcctataaa gtgaggggtg
    15781 cgatattcac ggtatttgac tccttggggc aggggttgcc ggaatcagca tatgaggcag
    15841 cattatctca caaactgatc atagccggat tgaatgttag acgtcaggtt tcattgccat
    15901 tggtatatga tggcgtcaaa atggatatcg gatatcgtat cgatctgctt attgaagatc
    15961 ttgttattat agagataaag tctgcagaga atctcactaa ggtccatcac aaacaattac
    16021 ttacatatct caagttatcc ggccacaagc ttggcatact agtgaatttc aattgcagcg
    16081 atatcaccaa aaacattttt cgaaaggtca actatttata aaccatattg agcgaaccat
    16141 tgcttgtcaa ttaatcaaaa ctacatccaa ctaacattcg aacacttttc tgtgccaatc
    16201 tgcgagatct gcaggacatt ttcaatatct ccatccaaac taatcgcttt aaacatagta
    16261 tgaaatgaat aaataagtat tgtgaagttt atggtgcgtg tttgtttacc gctgattacg
    16321 cagattttcg cagagtggtg tggtgaagat tttggttgaa ataaaaaaag gagactgtga
    16381 tgcagtctcc ttttttttac tgatgtaatg atcaattaaa tgttggtttt tcaggcagcg
    16441 tatatgttct gccggcgttt tgttttttca ggtaatccat caacggcatg aaataatcca
    16501 acattggttt tgcactcatg tcggagccta tagaagcctt gaggtgttcg cgccaatcaa
    16561 cggttgcacc aggcgtcatt accttgcgaa ggaaatcgcc tgtttctttc gaaccccaat
    16621 aattggtagc atgcggatct tgatgcaaaa tattttttgc gatataatca tggaactgga
    16681 acaacaatac attgctgata ctgtaatcat aatactgtgc aggatcatca ttgatatgtg
    16741 tttttgttgc agcatcacag tagtgctcat cgcgcggtga cggcggaaca ataccttgat
    16801 attgcttcac taattcccac cattttgcgt tgtattgatc aggagaaagg tttttcgcat
    16861 ataatgcata ttcgaattct gtcataacac cacttcccca agggatatga acaatatagt
    16921 caagtgcttc acttaataat ttcaatgtgt catttgtaac aacacccggt ttgatcaaac
    16981 cttgattttc aagcaagggt ttttgcaaac tggcgagacc aatcatagtt ccaaacgctt
    17041 catgatatcc gcgattggca cctgtgcgca aaaccggagg aacatcagga ttagaatagc
    17101 tcatataata gtagatatgt ccaagctcat gcaatacggt actccaccat tcggtattag
    17161 cttccacgct cataagtgaa cgcacatcct tgtcgagatc gatatgccag gcgctggcgt
    17221 gattattttt tgcgtatccg gcatcagcac tcacaggata taatgatgat ttttcccaaa
    17281 atgattgcgg caatgcgtca aatccaaggc tcatccaaaa tgcttcacct tgtttcatca
    17341 cccattcggc atcgtgcttt tttaattccg gatctatatc taatccttct acattaacca
    17401 ttgcagtcca gtcttgtccc cagcggtttg gcaaccattg tgccgggata taatcaggaa
    17461 caggttgatt atatttttct gccagctcat agcgtgccca agtatgcaat tcgcgatata
    17521 gcggccaaac atctgtgata aagctgcgtg taagttcaag catctcttcg ctggtcatat
    17581 tatattcact ggcgagataa gcgaagaaat cggaataacc gagaggtgtt acactttcgt
    17641 tgcgcaattt ttgtaattca acaagaccat cttttaggcc tacgccaact tctttactgg
    17701 ctgtccatgc ggcaagacgt tcattcatat tatttgaact gcgcaaaatc tcatccagtt
    17761 gattggtagt tacaggctta ccattaaggg tatatttaaa gccaaatagt ttttctacct
    17821 gagcatttgt tgctgtgatt cgatttttca caacatcgcc cgctgtttcc ggattattac
    17881 ctgccaggta gcggatatat tccaattgac gaacctgaat agctgtaagc ttatctttca
    17941 tcgctaaata tttctgcgct gtttcaatat ttgttttgct tccagtgaat ttggcgtatg
    18001 cttcatcggc gattgcagca ttgtgacttg ccattgtatc gccttcaacg atatgtgtct
    18061 gtaatatcca ctgtccttca gaagaagcgg ttacaagttt ttgatactcg gcgttatatc
    18121 cgtcgagata agtttgcacc tctttctgaa ttgcgctgtt atcttctgcc ttttcttttg
    18181 gtttgcagga aatcaatgct attggaatga ggagactcag ggtgagtagt tttctcatat
    18241 tggttggtat tttactgcta aggtaatgaa gctattgtgg cttttacagg ctcttaacac
    18301 accttaatta tctcgttttt tttgcacaat atgttcacaa cctaaagatt ttttctacgg
    18361 aagggcagga aatctttagc ttcgcatacg cttgcgcaat gtgcgtagca attcacttaa
    18421 tccttaaaaa caagaacaaa atggcagaag caaccaacga caaagtaaaa gccctagggc
    18481 ttgcagtaga cagtttgaat aagctgtatg gaaaaggcac ggtaatgaaa ttaggcgagt
    18541 cagccgtaac agcagaagag gttattccta cgggatctct tggagtggac atggcattgg
    18601 gcataggcgg tattcctaaa ggccgtgtag tagaaatcta tggccctgaa tcttcgggta
    18661 aaacaacatt ggcgattcat gtgatcgctg aggcacagaa aaaaggtgga atttgcgcga
    18721 tcatcgatgc cgagcatgcc tttgataaag tgtatgccga aaaattgggt gtggatgtag
    18781 agaacctgct tatctcacaa cctgattacg gtgaacaagc actcgagatt gctgatcatt
    18841 tgatccgctc cggcgcatta gatgtagttg taatcgactc agtagctgca ttggttccaa
    18901 aaggcgaatt ggaaggcgag atgggcgaca gcaaaatggg acttcaggca cgactgatga
    18961 gtcaggcttt acgtaaactc accggtacga tcaacaaaac cggctgttgc tgtattttca
    19021 tcaaccagct gcgcgaaaag atcggtgtga tgttcggtaa tcctgaaact acaacaggtg
    19081 gtaatgcatt gaaattttat gcatcagtac gtatcgatat tcgtcgcatt ggtcagttga
    19141 aagacaaaga cgatgtgatc ggtaaccgtg tgaaagtgaa gatcgtaaaa aacaaagtag
    19201 ctccaccttt ccgtcaggct gaattcgata ttatttatgg tgaaggtatc agcaagatcg
    19261 gtgagatcat tgaccttggt gcagaaagcg gtgtgattca aaaatcaggc agctggtata
    19321 gctatgaagg caccaagctc ggacaaggac gcgaaggtgt taaagatatc cttcgcgata
    19381 atcccgaact cgccctcgag atcgagaaaa agatcaaagc aaaattgtcg ggtgtgccga
    19441 ctaaggaaga ttaactacac aaaagaggcg gttgcaaaca accgcctctt ttttttgggg
    19501 cctcgcccca aaagtcgcag ggttcgcccg cggaaaagga ttttcattgg atgaaaaatt
    19561 tccctcgcat tataaaacac cttaaaaaaa gaattaccca ccgcgggcaa ccctgcgcct
    19621 aagcatcaag ctcaacacac accaacaggg ttgccttttt cttacgaaga gagattttct
    19681 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagggttgc ctttttctta
    19801 cgaagagaga ttttcttgca tgtaagattt ttttccgaag cggaattttt atgcgcgaac
    19861 cctgttgggt gtgtgttgcg ggggaggggg gcatggtggt tgcttcgcaa ccacgaaaaa
    19921 ttgtgagatt tttctaagcg cttaaagcgc tcgtgataat atgcatagcc gcgcgaagcg
    19981 cgtataagca tgtatgtttt atgaaatggt gtgaattcat tataggtgta tgtattttta
    20041 tttcatgact acatttaaaa ctcgtgatga acaggatgtc atttggttta tcacatttac
    20101 ttgctacagg tggttaccat tgattgaaac gaccgattta tatgatgata tctacaaatg
    20161 gttcgatata ttgaaaagcg aagggcattc cattttggga tatgtcatca tgcccaatca
    20221 catgcacatg ctcatgtatt tttctaaaaa taaaagaaca ataaatacat gcatggctga
    20281 aggtaaaaaa ttcagggcct gggaaattat taaaagattg aaaaaacaaa gggcatcaca
    20341 ttatttaatt cagatggaat gggtgcctga agaccataaa aaccctaaca aacagaagtt
    20401 catggccttt gaaccttcct ttgattgcaa ggtttgtaaa agcgaagaac gcgtgctgac
    20461 aaaacttaac tatatccata aaaatccggt aaacaaaggt tatcacctgg ccccttcaat
    20521 gactgcatac aaacattcaa gtgcaaaata ctatcacaca ggcatccact ctgcctatcc
    20581 tgtcgaacat tataagcgat ttatcaggcc atttaactgc aatatagatg cccctcaata
    20641 aaaattataa aattttaatt caatcactat tggttcgttc atctgaaact tcaaaaaact
    20701 cagatatttt gaatttttgt catattaaat tcccatgtta tgaatatcaa agctaattgc
    20761 tacactctcc tgatagcctt acttacgaga attcctgcat ttggtcaact cgaagcagta
    20821 gacacaataa gctacccggt tgattctgag cattcgacct atgtctttca aaatctaggg
    20881 aatgacggat acattctgat gtacaaaagc aacagcgata gggtagaatt atcgaagtat
    20941 gatactctga ataacgaaac aaatcacaat tcctttgagg ttgtcgcaca tctaagatat
    21001 gctgtatatc cagataaatc ctatagtgat tactttgata tctctgaaaa caacgaggtt
    21061 gtcaatcata gaattgactt caagacacta gatgttgtga caacaaagtg ttctgttgac
    21121 ataaaactca aatacaaata ccaggtttcc aataacgact gtatactcct tgtatttgaa
    21181 aattatttca agcaggaaat atataaagta gatatcaaaa caggtcaggt caatagaata
    21241 atatctgatt tgccggaaaa aaagaaaaag tcatttgcct atcaacaaat tacagttgta
    21301 ccatcgaccg gtgaattctt tttatttacc aacaaacaaa aattcttcaa gacatacaac
    21361 ctctccgcac accttaataa tgaaggtgag caaataggag cttacgtgtt gaaccccaaa
    21421 ccgtcgaaat tttatcaaaa ttacacagtg gtcgcaacgc ctgataatga ataccaaatt
    21481 tggtgtgaaa tgaagtatat taaaaaatta tccgattatg gtaatcccaa aatttcgggc
    21541 ttatgtcaaa ttgtaattga tgacgaatca gttgtaagcc ggaaatcaat tccatttcgc
    21601 tccttcaaga acttttatgc atttcaaaag gataataaat cgaaaaacat agataaaaac
    21661 tcccttaaat atcgcatggg ccaccctacc aaggtaaata tgcatacaat tcaaataaaa
    21721 cttgttgatg atgctgtata ctacgccgga gaagtatact ggccggtata taaaacagtt
    21781 gttgagcaaa ccgtgggttc aacagccaaa cctaccaaaa aaatggtatt agattatgtt
    21841 atttactctc atgcagttat cgcaaaattt gacctagaag gtaatatgat ctggaataat
    21901 tcagtagaat tggacgtaga tgatactgaa gccgttttgc aaatcaataa gtatcaaata
    21961 aataaaaatg gagtgggagt ccttctgatc aatgaaggag cgatcacatt gacaaaattt
    22021 gacgctgacg gtagaattgt ctccaaatac aacaccccat gtagcgcacc tatttgcgtt
    22081 gacgaagaaa aaaggaaatt taatttcttt agtctccaat attggtacaa tgattacatg
    22141 gtgtaccctg agtatacgat cgatgatgac ccctctacag agcgaaccga aggctactta
    22201 aatttgtaca aatacgaatt gatacaggat taagtccgca gacaggcatc aatcaagttc
    22261 ccataaaaca agaaaggtcg cgccctatcg gggttcgaaa ccatctactt aaaattcatg
    22321 atgtatcatc gaatgatcaa ttaaatctat aagcacgaag aatcaattga tcttaagata
    22381 agccgcaatt ggtaaattta caatctatca cttcaaatac ttcaacttaa caacctcccg
    22441 ctcagtaaga tgcctccacc ttcctcgcgg cagatcgcgc ttagtgagac cggcataaag
    22501 cgtgcgatcg agtcgaacaa cttcataacc caagtgctcg aatattcgtc gcacgattct
    22561 atttttcccg atgtggattg aaataccgat tacatttttc ttagccacat gagcgtagtc
    22621 aataccatca acataagcaa caccgtcttc caactcgaca ccatctgcta ttttttgcat
    22681 atccgtttgt gacaactgct tatctaattc aacttcataa agtttttgcg caccatattt
    22741 cggatgcatt aacttttgtg caagttcacc gtcgttggtg agtaacaata accctgttgt
    22801 atttctatcg agtcgaccaa ccggatatat gcgttcagta gtggctttcg caacaagctc
    22861 catcactgtg tgtctgccgc gatcatcatc caccgttgta atgtaatctt ttggcttatt
    22921 caggagcaca taaacataat ttacaggctt taccgctttt cctttgtaag taaccttatc
    22981 atcaggtttc aatcgataac ccatttcagt aactataaca ccatttactt tcactaatcc
    23041 ttctgcaatt atttcatc
//
LOCUS       DAOQOU010000037        22088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1133_length_22088_cov_6.219171, whole genome shotgun sequence.
ACCESSION   DAOQOU010000037 DAOQOU010000000
VERSION     DAOQOU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22088
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1133_length_22088_cov_6.219171"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2647)
                     /locus_tag="PLM90_05120"
     CDS             complement(<1..2647)
                     /locus_tag="PLM90_05120"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HMZ88782.1"
                     /translation="MKYRFITVAMLFSVGYAQVADQDLTKYVNPFIGTGGHGHTFPGA
                     TLPFGMVQLSPDTRPDGYNDWDGCGGYHYSDSIIYGFSHTHLSGTGVADYCDILLMPT
                     VGNIQLNNISGTDPLSGYASAFSHNTEHAEPGYYEVVLADDNIPVALTGTLRTGLHQY
                     SFPKTNQANVILDLRHRDKLLDGSYIEIVSPTKIQGLRRSSSWASDQWVYFAIEFSQP
                     FTVAGVVDSATTLKTAGTGFRPLILSGNQLHGYFRFNTDGAHPLMVKCALSPVSCAGA
                     WNNMATENPGWDFNAVRNNAKTAWNKELQKISIESPVDEARSLSDPDNKLVIFYTALY
                     HCMIAPNIYEDVDGKYRTRKNQIGITLNHTQYTVFSLWDTYRALHPLFTIIEPQRTND
                     FINTFLQQYQDGGDLPVWELSANETNCMIGYHAVSVIADAAVKGYSGFDKSLAFEAMV
                     HSANADRFGLKPYRLKGFIESTEEPESVSKTLEYAYDDWCIAQMSLLVSNKNINNDEY
                     NPTYDNFMNRGFGFIHLINENGFAQPRYNGGWYEPFDPFEVNFNYTEANSWQYSFAFP
                     QYLPFINTLEKKKQLEQQLDNLFSAKAKTSGREQADITGLIGQYAHGNEPSHHIAYLY
                     DYAGTPWKTQEKIHEICSKFYKNSPDGLIGNEDCGQMSAWYVMSALGIYQVCPGRPEY
                     ALTTPLFKKAVINNESPLIIQSNGDAEKNIYIHQVTLNGNNILVPIISHQQLMHGGTI
                     AYTISSATNIAWGTENMYTAALPKLPNKAPVLSPSKSTFENSTTISILCGANETIEYS
                     LNDGPLLTYTTPIKITENTVIKARAFNNTTNQSSPTVTGKYFLIPHNYNVTYQTAYNS
                     QYTGGGNTALIDGLRGNIDYRVGQWQ"
     gene            2830..4089
                     /locus_tag="PLM90_05125"
     CDS             2830..4089
                     /locus_tag="PLM90_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88783.1"
                     /translation="MLFKQATAILVCCASVLQISAQKDCSNDHLGRTPLIDLTDGYFN
                     GIRGGLYPEGSNIRPAEHLTECIEHVQAITPMDIYGNPNPNGKVVMLGIGASNPMTEF
                     QQFIDFSNDYEPVNDNLKIINACVGGQGIQKMFDITDNYWSGVVNGLEDQGLSAEQVQ
                     VVWIEQDNTRAYDTIFPSAPNALVNDYQLLLHVIHELFPNVQICYITARAYSGYADPV
                     DPEVSGGLLYPRDYFNGWAIKFLIEKVINHAFGFESEGPSAQIPLVTWGTYHWTDGST
                     PREDGLYLDCDLDVGPDGLHLSGVGEYKMGSLLFDYFKTDTTAKYWYLDEDFTGITTP
                     ETDVELRVWPNPVKNGTIHVDIPSTLIGADTQWMIYDMAGRAVLAGKEISGTELNIDV
                     TTLADGMYTFNCRDAQRQYHARMVIAH"
     gene            complement(4150..5100)
                     /locus_tag="PLM90_05130"
     CDS             complement(4150..5100)
                     /locus_tag="PLM90_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ88784.1"
                     /translation="MQDVVLGIDIGGTGIKFGLVTRTGEILVTDKVKTKGYPNPEKLP
                     EDLLKWATKACSDHQVTLVGVGIGTPNGNYYKGTIDFAPNLPWKGVVTLKSFFESTFK
                     LPAALTNDAKAAAIGEMYFGGAKGMKDFLFITLGTGLGSGIVVNGQIAYGFDSLAGEV
                     GHTIVYRNGRECPCGRRGCLEQYVSAPGIVKTYYELLRSKNLDEFRTSIGNMIDSAEL
                     CKLAEDGDTLALEAFQKTGEILGFALSNSACYTRPEKIFLFGGLAQAGDLIFKPTIKS
                     FESYLLPIYQNKIPIVPSGLKESEAAILGSASLIWNELNK"
     gene            complement(5118..5564)
                     /locus_tag="PLM90_05135"
     CDS             complement(5118..5564)
                     /locus_tag="PLM90_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ88785.1"
                     /translation="MTPQELLAIYRKNLESTIQEVTNFPDDTMLWETAPGVLNSAGNL
                     ALHIAGNLRYFLGNEIAGTGYVRDRDAEFNTKGLSRSEVCDALRLAIADVELTLANMP
                     KEMLGNNYPLDRFGGVQTTGFVLTYLLAHLNYHLGQVNYLRRILAK"
     gene            complement(5568..6557)
                     /locus_tag="PLM90_05140"
     CDS             complement(5568..6557)
                     /locus_tag="PLM90_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HMZ88786.1"
                     /translation="MRQTPFFEIYLALFLLLGTLCFGVIGYMVLEGYNLVEALYMTVI
                     TLGTVGFTEVRPLDDAGRMFTIVLILAGLVIFAYFITQISRYFLDGEFILHYKSYKMK
                     KQIEGLKNHVIICGFGRNGREAARVLLKSNMHVVVIDQDESKLAEFAEEAHFYYIYGD
                     ATRDETLIDANITQASAILTTLPDDAQNVFVCLTAKELHQNIKIIARATHLSSVRKLK
                     NAGATNVIMPDKIGGAHMAMLVSNPDIEEFIDIMATHSQDDFSIQEVMAGKRVNLGDI
                     NCWQKTGATVIGIKSSGNQYILNPDANVSVNPGDGVIIMGSKIQINASKQLFI"
     gene            6779..7546
                     /locus_tag="PLM90_05145"
     CDS             6779..7546
                     /locus_tag="PLM90_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ88787.1"
                     /translation="MDLSIFMHADAWIALLTLTILEIVLGIDNIIFISILSTKLPEAQ
                     QKPARRWGLMLAMGTRVLLLLSISWILGLEADLFNISGHGISGRDIVLLIGGFFLIYK
                     ATTEIHGKIEGEEHETSSKAKTPSFVSVVGQILLLDIVFSLDSVITAVGMVDADKVII
                     MIAAVIIAVIIMLFAADPISNFVQKHPTVKMLALSFLVMIGVSLIIEGWGGHVEKGYI
                     YTAMGFSVVVEMLNLRMKANAKKRHELRNMKENEFGN"
     gene            complement(7550..9367)
                     /locus_tag="PLM90_05150"
     CDS             complement(7550..9367)
                     /locus_tag="PLM90_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ88788.1"
                     /translation="MKSYLRILKYVGRFKNYAILNFGFNLLYAFFNLFSILMVIPFLQ
                     IIFKMVEPPSQAPDFSWNIKQLMNYLNYYFAAYVSDHGAFAGLLIVCIAVVIMFLLKN
                     ICRYLALYYLAPLRTGIVRELRQGVYDKILQLPLSYFSEKRKGDIIARMSTDVKEVEN
                     SIMNTIESTFRDPVTMICFLIAMFVISFELTIFVLLLLPIAGLLIGRIGKSLKRKSQK
                     EQGQLGYLMSLFEESLSGLRIIKAFNAERYQKGKFLRENKQLEALATSAHRRWNVASP
                     LIEFLSVCVVAALLYYGGSIILRADSGLTADLFLGYILVFANMLGPARNVANAYFYIQ
                     RGIASLERIESIMNAEVQIKESLHATNIRTFDHAVEYQDVVFAYNETPVLRGINIKIE
                     KGKMIALVGPSGSGKSTFADLLARYHDVTSGSIRIDGNDIRDLRISSLRNQLGIVTQE
                     SILFNDTVFNNIAFGIEHASEEDVIKAAKVANAHDFIMRLENGYQTMIGDRGSKLSGG
                     ERQRITIARAIYKNPPILILDEATSSLDTENEKLVQDALFKLMKNRTSIVIAHRLSTI
                     QFADEIVVMQEGRIVERGNHNALIAKNGLYARLVEMQAF"
     gene            9473..9835
                     /gene="rbfA"
                     /locus_tag="PLM90_05155"
     CDS             9473..9835
                     /gene="rbfA"
                     /locus_tag="PLM90_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HMZ88789.1"
                     /translation="MDSRRQKKIGQLIQDEMGTFLQKEGANYYGRKFVTVTDAQVTPD
                     LQVCRIYVSVLDKNEGEKVVDSLNLHVRDIRKRFGQIMRQTLRIIPELEFYLDNTLEN
                     VFRLEEIFREQKKNEADQ"
     gene            9845..11086
                     /locus_tag="PLM90_05160"
     CDS             9845..11086
                     /locus_tag="PLM90_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ88790.1"
                     /translation="MKFTLRIAWRYIFSRKSTNAINIISGISMSGIMIGSAALIIVLS
                     AFNGFENLVQRLYNTFYPDITVTPSYGKVFSPDSLRLQKIAHIDGVQDMSMTLEENAL
                     LAYNDQEHIATIKGVDTIYKKVTAVDDSVFYGQYVLEYEYLHTQIDCAVLGSGVASTL
                     NVALGLDNPSVQIFMPRRGTKGISNIQNTFIQRSIQPMGVFRIQQEFDSKYVITSLDF
                     IRNMLEYQNNEISAIEISVKGRAVDDVMDDIESIMGNEFTVKNRFMQNEFLYRVMRTE
                     KWAVYFILTFIFIIAAFNMIGSIAMLVIDKKKDIGILRSLGASEKSVRNIFFIQGILQ
                     TLVSIAVGFTLATLLCFLQMHFGLITIPGEGTFVVTAYPIALEWRDYVSVFLTVFIVG
                     CIASWFPAYMAARQRWLFKSE"
     gene            complement(11136..13811)
                     /locus_tag="PLM90_05165"
     CDS             complement(11136..13811)
                     /locus_tag="PLM90_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMZ88791.1"
                     /translation="MKLQPFIAVALAAALFYQCDPNKHIYKSEPYLGTIDTSGAAAYD
                     AYDYGYDSDAVGDYDYDYSYDYQNDTAYSPYSNAIYRGSSKREFDLLHTKLDVSFDWS
                     KAWMYGKATLTLKPYFHPMSTLTLDAKGFTVKEVSLVTATGRTPLKYVYNTDTIPDTL
                     QMTIDLGKTFKKDESFMIYIDYIAKPNDLPLGGSVAITGDKGLYFINNDGADPNKPMQ
                     IWTQGETEATSCWCPTIDKPNERCTTDFSITVADKYQTLSNGSLISSKKNTDGTRTDN
                     WKMDLPFAPYLFMMAVGEYAIVKDTWNKMQVNYYVEKDYEQDARAIFGNTPEMIDFYS
                     KKLGVNYPWPKYSQIVVRDYVSGAMENASATLHGEFLQQHKRELLDENYEDVISHELF
                     HHWFGDLVTCESWSNIPLNESFATYGEYLWREYKYGRDDADYIGYNDLQTYLNEASYK
                     MVDLIRYNYDTREDMFDSHSYAKGGRVLHMLRKYLGDDAFFAGLNLYLEKNKFQSVEI
                     HDLRKAMEEVSGEDLSWFFNEWFLDKGHAELSIYYEWFPEEDKLDITIYQNQDTSVAP
                     VYVIPMDVDFYYNGKVERKRITLDRSMQTFSFNFPVQPDLVNVDAEKMLLGNKYDNKD
                     IDSYIFQYQHAPLYRDRAESLEALAPYQAEDPRYRDLITSALDDKHWSLRQFAIDTLL
                     IDASTPQSVKEKIMQMAKSDPRSYVRSSALNKLKEITGIDAWSVYNAAMNDSSYNVVA
                     TAMTLIYNNDKKKGLELARTYKTEANDNLSYSIMGILSDGGEAIDNDYFLAKFKEASG
                     FEVYYVLMYYEPFILRMTDSYIIKKGVDELKKVASDQSSFWMSFTAVNTLSSMATTYQ
                     DAINNTTDLQMKQSLQSALDYVNAALTEVNGGGDY"
     gene            13834..14379
                     /locus_tag="PLM90_05170"
     CDS             13834..14379
                     /locus_tag="PLM90_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HMZ88792.1"
                     /translation="MESKVTDSMLSLTPKSANIMYNVTVNGTQVYKVDGNTVNGNEIL
                     ADVLKVDNRFYHILTGGKSFNVEVVRLDKTAKTMTLQVNGTKYEVSLRDKFDALMEQM
                     GFGSGAGQKAADLKAPMPGLVIEVRVQPGDQVKKGDPVIVLEAMKMENVLKAPGDGKV
                     KSIEVQKGVSVEKGLVLIKFE"
     gene            complement(14386..15342)
                     /locus_tag="PLM90_05175"
     CDS             complement(14386..15342)
                     /locus_tag="PLM90_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HMZ88793.1"
                     /translation="MSHLHIAKNVLLFESEQLRRTLDHLNDNFDAAVNAILQSKGKVV
                     VCGIGKSGAVAQKITATLCSTGTPAVFLHAAEAAHGDLGVYADGDPILMISKSGTTEE
                     MLRLIPFFLQSSSSVIAIVGNLNSPIARQSTIVLDGSVEKEADALNLAPTASTTVALA
                     LGDALAVALMHARGFTEKDFARFHPGGQLGKNLMLKVEDVMHEMPFIARVSEKNSFRE
                     LILAMTKFNLGAACVVNDAQELVGLVTDGDVRRSLLRDADLSSMSVSSIMTRNPVSVS
                     VHATLKEAVDLMENRSSQLSVLPVVEGKTCMGLIRIHDIYQG"
     gene            complement(15499..16485)
                     /locus_tag="PLM90_05180"
     CDS             complement(15499..16485)
                     /locus_tag="PLM90_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ88794.1"
                     /translation="MNSNHKPRVLITGAAGFLGSHLCDRFIAEGMHVMAMDNLITGDK
                     RNIEHLFELSNFEFYHHDVSQFVHVKGDLDYILHFASPASPIDYLKIPIQTLKVGSLG
                     THNLLGLAKAKQARILVASTSEVYGDPLVHPQSEDYWGNVNPVGPRGVYDEAKRFQEA
                     ITMAYHTYHGLETRIARIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTVFGDGSQTRS
                     FCYVGDLIEGIYRLLMCDYAQPVNLGNPDEITINEFAEEIIKLTGTTQKIMHHPLPVD
                     DPKQRQPDISLAKKILGWEPKVHRSEGLQITYQYFQQLSKEDLYRDIEGRRF"
     gene            complement(16592..16981)
                     /locus_tag="PLM90_05185"
     CDS             complement(16592..16981)
                     /locus_tag="PLM90_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ88795.1"
                     /translation="MGSFKSFEEMAVWLEARKVSQDIYLLTKKAEFSEDPKLRWQMRG
                     SSGSIMDNIAEGFSRGGTKEFIQFLRIAKGSSGELISQLHRCNDRNWISNNDFVTLCA
                     KTQKITGMLQSLIQYLQSTTRKGPTHQ"
     gene            complement(17122..18423)
                     /locus_tag="PLM90_05190"
     CDS             complement(17122..18423)
                     /locus_tag="PLM90_05190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HMZ88796.1"
                     /translation="MKISVIGTGYVGLVTGTCFAETGNHVICVDIDAAKVQRMQQGEI
                     PIYEPGLETIFHRNIRQGNLQFTTDLKSAVEATDIIFLALPTPPNEDGSADLKYVLQV
                     AEDLGKIITSYKIIIDKSTVPVGTADMVRALIAKHCTTEFDVVSNPEFLREGMAVEDF
                     MKPERVVIGTESERAKEMLGRLYAPFVRQGNPVIFMDERSAELTKYAANAFLATKITF
                     MNEIANLCEILGADVDMVRKGIGSDSRIGKRFLFPGVGYGGSCFPKDVKALNQMAGVN
                     GYDFHILRSVMAVNEKQRVILADKVTSHFGNDLSGKHFAMWGLAFKPNTDDIREAPAL
                     YLIDALLERGATITAFDPEAMPNVEKQIGKRINYAVNQYEALSGADALIIVTEWSVFR
                     TPDIEAIKSALKSPVIFDGRNVFEVAEMKQAGIVYYSIGRV"
     gene            complement(18502..19587)
                     /gene="prfA"
                     /locus_tag="PLM90_05195"
     CDS             complement(18502..19587)
                     /gene="prfA"
                     /locus_tag="PLM90_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HMZ88797.1"
                     /translation="MHDQIDLTGKLQAIKERWLNIQEQLSDPDIIADMKRFKSLNKSY
                     RDMEPVVAAYERYQLVLSNLDDSRTILNTERDAELRDMAKEEIAAYETEKATLEEEIR
                     QLLIPKDPEDEKNAIIEIRAGTGGDEAALFARDLSEMYTRFCGKQGWRIEIMNENASD
                     QGGYKEIVLEVSGENVYGRLKYESGVHRVQRVPETESQGRVHTSAATVAVLPEAEEFD
                     VEINATDVKVDVFRASGAGGQHVNRTESAVRMTHIPTGIVAECQDERSQIKNREKAMK
                     VLRTRVYEKFHNEHLEKISKQRKTLVSTGDRSAKIRTYNFPQSRVTDHRINLTLYNLP
                     EVINGEIDEIINALQVAENAEKMKAEA"
     gene            complement(19662..20447)
                     /locus_tag="PLM90_05200"
     CDS             complement(19662..20447)
                     /locus_tag="PLM90_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88798.1"
                     /translation="MKFSILGIICMLQLQLGATDSLVCTIAVKPSYFTVDANGMLYYV
                     TASNEISRTDTCGNHTITYSNIANGTPTLIDVGNPLKILAFYKDQQKVVVLDKTMSEI
                     AVLSFNNINGKSYMPSLLCRATGGDHIWLFDEFTQSFVRLDESGNKIAASEPIYQIFE
                     KSEVPLWMVDIQDHLYVYTSGGLIYLFDAYGTPGTTFMLNDAPFSAYRNMFLIKGPTG
                     FSLVNIFTNEVIALPLPATGDVRLQNDLMYYYDGDHINVLQFR"
     gene            complement(20437..21180)
                     /locus_tag="PLM90_05205"
     CDS             complement(20437..21180)
                     /locus_tag="PLM90_05205"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HMZ88799.1"
                     /translation="MERTKIYFASDFHLGIPNHSVSLEREKKIVRWLDSIKHDAKQIF
                     LLGDTFDFWFEYDHVVPRGYVRLLGKLAELRDSGIDIQAFTGNHDLWMFGYFEEELNI
                     PVHHHPIFIESHGKKFYIGHGDGVGPGDRGFKLMKSVFTNRFAQFLYSLLHPYLAFKL
                     ARYFSHNSRMLNEEETFLGEEKEWQIIYAKEMLQKEHIDYFLFGHRHIVLELKLSDNS
                     TFINLGDWVRHYSFAEFDGQTCKIKYFEV"
     gene            complement(21167..21838)
                     /locus_tag="PLM90_05210"
     CDS             complement(21167..21838)
                     /locus_tag="PLM90_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HMZ88800.1"
                     /translation="MAKILSSKEKMLSSVRGALLHQTRQPYPNLESNDNIYNVPDEPL
                     EIIFATEFTAVSGQFVFCETEKECVDTLKELVAHKKWEHIVCPEPLLHELFDRQQFIR
                     YDADAPVTGADAGIMLCEALIARTGTILISSRQASGRTLPIFPEVNIVIASTKQLVLD
                     IQDGFAAIRKHYDGNFPSMVNLNTGPSRTADIEKTLVLGAHGPREVYVFLIDNWPMAG
                     WDGKN"
BASE COUNT         6315 a         4570 c         4462 g         6741 t
ORIGIN      
        1 cttgccactg tcctacacgg taatcgatat ttccccgtaa gccatctatg agggctgtat
       61 ttccaccccc tgtatattga ctgttatatg cagtttgata tgtcacgttg taattatgtg
      121 gaatcaaaaa atattttcct gttaccgtag ggcttgattg atttgtagta ttattaaatg
      181 cacgcgcttt tatcactgta ttttctgtaa tctttattgg cgttgtatat gtcagtaaag
      241 gcccatcatt taaactgtat tctattgttt cattagcacc gcacaagatt gaaatggtag
      301 tgctgttttc aaaagtactt tttgatggtg atagtacagg agctttattc ggaagctttg
      361 gcaatgcagc ggtgtacatg ttttctgttc cccatgcaat attggtagct gatgatatgg
      421 tgtatgcgat tgtgccacca tgcatgagtt gttgatgtga tattattggc actaagatat
      481 tattgccatt caatgtcacc tgatggatgt aaatattttt ttctgcatca ccgttcgatt
      541 gtataataag tggactttcg ttatttatca cggccttttt aaataaagga gtagtgagtg
      601 catattccgg ccttccgggg caaacctggt aaatgcctaa ggcactcatt acataccagg
      661 cactcatctg accacagtct tcattcccga ttaatccatc aggtgaattt ttgtaaaatt
      721 tgctgcatat ctcgtgaatc ttctcttgag tcttccacgg cgtgccggca taatcatata
      781 aatatgcaat gtggtgactg ggttcattgc catgtgcata ctgaccaata agtcctgtaa
      841 tatcagcctg ctcgcgtccg gaggttttgg ctttcgcact aaataaatta tctagctgtt
      901 gttctaattg tttctttttt tcaagtgtat taataaatgg taaatattga ggaaacgcaa
      961 atgaatattg ccagctattg gcttccgtat aattgaaatt cacttcaaaa ggatcaaatg
     1021 gttcatacca tccgccattg tatcgaggtt gcgcgaagcc attttcattg ataagatgaa
     1081 taaaaccaaa tcctctgttc atgaaattgt cgtaggttgg gttatattca tcattattga
     1141 tatttttatt tgaaacaagc aatgacattt gagcaataca ccaatcgtcg taagcatatt
     1201 caagcgtttt agatactgat tccggctcct cggtgctttc aataaaacct ttcaagcgat
     1261 agggcttaag gccaaatcta tcggcatttg cagaatggac catcgcctcg aaagcaagag
     1321 atttatcaaa tcccgaatac cctttaactg cagcatcggc aatcacactt acggcatgat
     1381 atcctatcat acaatttgtt tcatttgcac tcaattccca aaccggaaga tcaccaccat
     1441 cctgatactg ttgtaaaaaa gtattgatga aatcattggt gcgttgcggt tcaattatcg
     1501 taaataatgg atgcaatgcc ctgtaagtat cccataatga aaatacagta tactgtgtgt
     1561 gatttagtgt aatgccaatt tgatttttcc ttgttcgata tttgccatca acatcttcgt
     1621 aaatattagg tgctatcatg cagtgataca gtgccgtata aaaaataacc agcttattat
     1681 caggatctga aagtgatctt gcctcatcaa caggactttc tatgctgatt ttttgtaatt
     1741 ctttattcca tgcggtttta gcattgttgc gtacagcatt gaaatcccaa cccggatttt
     1801 ctgttgccat attattccac gctccggcac aactaacagg agagagtgcg catttcacca
     1861 tcaatggatg tgcgccatcg gtattaaagc gaaaatatcc atgaagttga ttgccactca
     1921 ggattagtgg tctgaaacct gtgcctgcgg tttttagcgt agtggcggaa tcaaccactc
     1981 cggcaacagt aaatggttga gaaaactcaa tggcaaaata aacccattga tcacttgccc
     2041 atgatgatga tcgacggaga ccttgaattt ttgttgggga tacaatttct atataggagc
     2101 catcgagcaa tttatcgcga tgtcgaagat cgagaataac attcgcctga ttggttttgg
     2161 ggaagctata ttgatgcaaa cctgttcgca aggttccggt aagggcaacg ggaatattat
     2221 catcggcaag caccacctca taatatcccg gttcagcatg ttctgtgttg tgcgaaaatg
     2281 cggaggcata tcccgaaagc ggatctgtgc cagaaatatt attcaactga atatttccta
     2341 ctgtaggcat cagcaggata tcacaataat cggcaacacc ggtaccgctg agatgtgtat
     2401 gtgaaaatcc ataaataata gaatcgctgt aatgatatcc gccacagcca tcccaatcgt
     2461 tataaccgtc ggggcgggtg tcaggactca actgcaccat gccaaagggc aaagttgctc
     2521 ccggaaaagt atgcccatgg cccccggtac ctataaatgg attcacatat ttcgtaaggt
     2581 cttgatcagc aacttgggca tacccaacag aaaagagcat tgccacggta atgaaccggt
     2641 atttcatgcg atgaagataa agaataatga tgtagatgcc ttgcacagct tggggaaaga
     2701 ttccccacat gggttataca aaaatttaac atccctttgt ggcactagtg ttttgagttt
     2761 cagcacgtta tttgtaatat tgatgatgac aacacaccgc atgtctatat tatcatcaac
     2821 caaaagccta tgttatttaa acaagcgaca gcaatattgg tgtgttgcgc aagtgtctta
     2881 caaatctctg cccaaaaaga ttgttcgaat gatcatctcg gcagaactcc tttaattgat
     2941 ctcaccgacg ggtactttaa tggaatcaga ggcggattat atccggaggg aagtaatatc
     3001 cgtcctgctg aacatttaac agaatgtatt gagcatgtgc aagcgattac accaatggat
     3061 atttatggaa atccaaatcc aaatggcaaa gttgtaatgc tcggcattgg cgcttcaaac
     3121 ccaatgacag aattccaaca gtttattgac ttttcaaatg attatgaacc ggtaaacgac
     3181 aatttaaaaa ttatcaatgc ttgcgtcggt ggacaaggca ttcaaaaaat gttcgacatc
     3241 acagataatt attggagtgg cgtggtgaat ggacttgagg atcagggttt aagtgcagag
     3301 caggtgcagg tagtttggat agaacaagat aatacccgtg cctatgatac catttttcct
     3361 tcggcaccaa atgcattggt aaatgattat caattgttat tacatgtaat acatgaatta
     3421 tttcccaatg tacaaatatg ctatatcaca gcacgtgctt atagtggtta tgctgatcca
     3481 gtagaccctg aagtttcggg cggattgtta tatccaagag attattttaa tggttgggct
     3541 ataaaatttt taatcgaaaa ggttatcaat catgcattcg gttttgagtc ggagggcccc
     3601 tctgcgcaaa tccctttagt cacttgggga acatatcatt ggacggatgg atctactcct
     3661 cgtgaagacg ggttgtatct tgattgcgat ctggatgtag ggcctgacgg attgcatctt
     3721 tcaggtgttg gagagtataa aatgggatcg ttgctttttg attatttcaa gacagatacc
     3781 actgctaaat actggtatct ggatgaagat tttaccggta ttacgacacc ggaaaccgat
     3841 gttgaattgc gcgtttggcc taatcctgtt aaaaatggca caattcatgt agatatacca
     3901 tcaacactga ttggtgctga tacgcaatgg atgatttatg acatggctgg aagagcagta
     3961 ttggccggaa aagaaattag tggaactgaa ttgaatattg atgtaacaac tttggccgat
     4021 ggaatgtata cattcaattg cagggatgca caaaggcaat atcatgcacg catggtaatc
     4081 gctcattaat tattcgtgac ccgtttatat atatgttaag agaatgccgc ctgcaaatgg
     4141 tggcattttt tatttattta attcattcca aatcaatgat gcgcttccta aaatggcggc
     4201 ctcactttcc tttaatccgg aaggtacaat tggaatttta ttttggtaaa tcggcaataa
     4261 atatgactca aaacttttga tggtaggttt gaaaattaaa tcaccagctt gggctaagcc
     4321 accgaaaaga aatatttttt ccggtcgcgt atagcatgcc gaatttgaca aagcaaagcc
     4381 aagaatttct ccggtttttt gaaatgcttc caatgctaag gtgtcgccat cttctgcaag
     4441 cttgcataat tcggcgctat ctatcatgtt gccgatgctg gttctgaatt catcaagatt
     4501 tttgcttcgc aggagttcgt aatatgtttt tacaattccc ggagcgctta catattgttc
     4561 tagacaaccc cttcttccac aagggcattc gcgaccattg cgatatacga tggtatgacc
     4621 aacttctccg gcaaggctgt cgaatccata ggcaatttgt ccatttacca caatgccgct
     4681 acccaaacct gttccgaggg tgataaataa aaaatctttc atccccttcg caccgccaaa
     4741 atacatttca cctatagctg ctgctttggc atcgttggtg agggctgcgg gtaatttgaa
     4801 ggtggattcg aaaaaagatt ttaaggttac cacgcccttc catggcaggt taggggcgaa
     4861 gtcaatggtg cctttataat aattgccatt tggcgtgcct atgccaacac cgacaagagt
     4921 tacttgatga tcggagcagg ctttggtagc ccatttcaaa aggtcttcgg gtagcttttc
     4981 ggggttcgga tatcctttgg tttttacttt atctgtaacg agtatttcac cggttcttgt
     5041 taccaaacca aatttaatac cggtgcctcc gatatctatc cctaaaacta cgtcttgcat
     5101 aacgcgataa aattaggtca tttcgccaaa atgcgacgca agtaattcac ctgacccagg
     5161 tgataattta aatgtgccag taaatatgtc agcacaaatc ctgttgtttg cactccacca
     5221 aagcgatcga gaggatagtt gttgccaagc atttctttgg gcatattcgc cagggtaagt
     5281 tctacatcag caatggcgag acgcagggca tcacaaacct ccgaacgaga aaggcctttc
     5341 gtattgaatt cggcatcgcg atcgcgcaca tatcctgttc cggcaatctc gttgcccaga
     5401 aaatatcgga ggtttccggc aatatgcaat gcaaggttgc ctgccgagtt gagcactccc
     5461 ggggctgttt cccatagcat ggtgtcgtct gggaaattgg ttacttcctg gatggtgctt
     5521 tcaagatttt tgcggtagat ggccagtagt tcttgaggtg tcatagctta aatgaatagt
     5581 tgttttgaag cgttaatttg aattttagag cccatgatga taaccccgtc gcccggattt
     5641 acacttacat tggcatccgg atttaatata tactgatttc ccgacgattt aattccaata
     5701 acggtagccc ctgttttttg ccagcagtta atatctccca gattcacgcg ttttcctgcc
     5761 attacttcct gaatagaaaa atcatcctgc gagtgagtag ccatgatatc aataaattct
     5821 tcaatgtccg gattgcttac aagcatggcc atgtgcgccc ctcctatctt atcgggcata
     5881 atgacattcg tggcgccggc attttttaat tttcttacgg aactcaggtg cgttgcgcgg
     5941 gcaatgatct tgatgttttg atgtaactcc ttcgccgtaa ggcatacaaa tacgttttgc
     6001 gcatcatcgg gtaaagtagt caggatggca ctagcttgcg tgatattggc atcaatcagc
     6061 gtttcatccc tggttgcatc gccatagatg taataaaaat gagcttcctc ggcaaattcg
     6121 gctaatttgc tctcgtcctg gtctatcaca accacatgca tattactttt caacaatacg
     6181 cgggcagctt ctcttccatt tcgaccgaat ccacaaatga ttacatggtt tttcaagcct
     6241 tctatttgtt ttttcatttt ataggattta tagtggagaa taaattctcc atccagaaaa
     6301 tatcgggata tctgtgtaat gaaataggca aaaatgacta atccggccag aattaaaacg
     6361 atggtaaaca ttctgcctgc atcatcaagt ggccgcactt cagtaaagcc aacagttcca
     6421 agtgtgatta cggtcatgta taaggcttct acaaggttgt atccctctaa aaccatatat
     6481 ccgattacac cgaaacacaa ggttccaagt aataggaata atgccaggta aatttcgaaa
     6541 aatggtgtct gacgcacggt attgctggat ttccggtaaa caggtgaaat agcacgtaag
     6601 gaaaaccgaa aaaaccgttc aagcgcgagt tttgagggct tttacggtaa aatcgattga
     6661 ttcagtagca tatttcgccc ttttcagcgt ctaaattacc tattaacagg aacatattaa
     6721 ctatttcctt ttttgcattt tgaacttttc atcacagttt tgccgctaaa cccaacctat
     6781 ggacttatcg atattcatgc atgccgacgc ctggattgct ttattgacgc ttacaatact
     6841 tgaaattgta ctcggcattg acaatatcat tttcatatca attctttcta cgaagctacc
     6901 tgaagcgcaa caaaaacccg cccgtcgttg gggtttaatg ctggccatgg gaaccagggt
     6961 gttattactg ttgtcgatca gctggatttt aggattggaa gctgatttat ttaatatcag
     7021 cggtcatggt atttcaggta gagatattgt tttattgatt ggtggatttt ttctcattta
     7081 taaagccacg accgaaattc atggcaaaat tgagggtgag gaacacgaaa cttcttcaaa
     7141 ggcaaaaacc ccttcatttg tatcggtagt tggtcaaatt cttttgctgg atattgtatt
     7201 ttctctcgac tctgttatta cggcagttgg tatggttgat gcagataaag taatcataat
     7261 gattgctgct gtaattattg cggtgattat tatgttgttt gcagcagatc ctatcagcaa
     7321 ttttgtgcaa aaacatccaa cagtaaaaat gcttgccttg tcatttttgg tgatgattgg
     7381 tgtgagcctt attattgaag gatggggcgg tcatgttgaa aaaggataca tctatacggc
     7441 aatgggcttc tctgtggtag ttgaaatgct gaatctccgc atgaaagcaa atgcgaagaa
     7501 aagacatgaa ctgcgcaata tgaaagagaa tgaatttggg aattgaaaat taaaaagcct
     7561 gcatttcaac gagccttgca tacaagccgt ttttggcaat taatgcattg tgatttccgc
     7621 gttctacaat acgtccctcc tgcattacaa caatttcatc ggcaaattga atggtgctga
     7681 gtcgatgtgc aattacgatg gaggtgcggt tcttcattaa tttgaataat gcatcctgaa
     7741 caagtttttc gttttcagta tcaagagatg atgttgcttc atccaaaata aggatgggcg
     7801 gattcttata tatcgctctt gcaatggtaa ttcgctgacg ttctccaccg ctgagcttag
     7861 atcctctgtc gccgatcatg gtttgatagc cattctccaa acgcataatg aaatcgtgtg
     7921 cattggcaac ttttgctgcc ttgattacgt cttcttcaga agcatgttca ataccgaagg
     7981 cgatattatt gaacacggta tcgttgaaca atatcgactc ctgtgttact attcctaatt
     8041 gattgcgcag acttgaaatg cgtaaatctc gaatatcatt gccatcaatg cgtatagatc
     8101 cggaagtaac gtcgtgatag cgggcaagca gatctgcaaa tgtagattta cctgatccgg
     8161 aaggcccgac caaggcaatc atttttccct tctcaatttt aatattgata ccgcgtaata
     8221 ccggtgtttc attatatgca aacactacat cctgatattc taccgcatga tcaaatgtgc
     8281 gaatattggt agcgtgtaaa ctttctttaa tttgtacttc tgcattcata atgctttcga
     8341 tacgttccag tgatgcaatt ccgcgttgaa tgtagaaata tgcattggcc acgtttcttg
     8401 caggacctaa catgttggcg aatactaaaa tgtatcctaa aaataaatcg gcggtaagtc
     8461 ctgaatctgc acgcaaaatg atacttccgc cataatagag taaggcagca acaacgcaaa
     8521 cacttaaaaa ctcgatcagc ggacttgcaa cgttccatcg gcgatgtgcg ctggttgcaa
     8581 gtgcttcgag ttgtttattc tcgcgaagga attttccttt ctgatagcgt tcggcattaa
     8641 aggctttaat gattcgcaat cctgaaagtg attcctcgaa cagcgacatt aagtaaccta
     8701 attgtccttg ttctttttgc gatttacgct tcagcgattt tccaattctt ccaatcaaca
     8761 atcctgctat cggcagtaac aataatacaa aaattgtcag ttcgaaactg atgacaaaca
     8821 tggcaattag aaaacatatc atggtaaccg gatcgcgaaa agtggattcg atggtattca
     8881 tgatgctgtt ttccacttcc tttacatcgg tgctcatgcg cgcaattatg tcgcctttgc
     8941 gtttttcgct gaagtaggag aggggtaatt gtaaaatttt gtcatatacc ccttggcgta
     9001 attctctcac aattcctgtt cgcaatggag ccagataata taatgcgaga tagcggcaga
     9061 tgtttttcag aagaaacata atcaccaccg caatacaaac aattaataga ccggcaaacg
     9121 ccccatgatc ggatacataa gctgcgaaat aataattgag gtaattcatc aattgcttga
     9181 tgttccatga gaagtcgggc gcttgggaag gaggttccac cattttgaag atgatctgca
     9241 gaaatggtat caccatcaaa atagaaaaca ggttgaagaa ggcatagagc aggttaaatc
     9301 caaaattaag gatggcgtag tttttaaacc gccccacgta cttcaatatc cgcaggtaac
     9361 ttttcatgat aggtcacaaa attacaaaaa cgaggggttc catacaccct tgcatctaaa
     9421 ctaaaagacg cgaaacgacg ttgtattgcc gaaaaccgta acttagcaga ttatggacag
     9481 cagaagacaa aagaagatcg gacagttgat ccaggatgaa atgggcacct ttttgcagaa
     9541 agaaggagcc aattactatg gacgaaaatt tgttaccgta accgatgctc aagtgacgcc
     9601 tgacttgcag gtatgtcgca tctatgtgag cgttctcgat aaaaatgaag gcgaaaaggt
     9661 tgttgattca ctaaatctgc atgtgcgtga tatccgcaag cgatttgggc agatcatgcg
     9721 acaaacactg cgtatcatac cggagttgga attttacctc gataatacgc ttgaaaatgt
     9781 atttcgtctg gaagagatat tccgtgagca gaagaaaaat gaagctgatc aataacgctc
     9841 ttgcatgaag tttacccttc gcatagcctg gcggtatatt ttttcaagaa aatcgacaaa
     9901 cgccattaat atcatctccg gcatttccat gtcggggatt atgataggca gtgccgcact
     9961 cattattgtt ttgtcggcat tcaatggatt tgaaaacctg gttcagcgat tatacaatac
    10021 tttttatcct gacatcactg ttacaccaag ttatgggaag gtgttttcgc ctgactcatt
    10081 gaggctgcaa aaaatcgcac atattgatgg tgtgcaagat atgtccatga cgcttgaaga
    10141 aaatgcatta ttggcatata atgaccagga acatattgct actattaaag gtgttgatac
    10201 catttacaag aaagtaactg ccgtagatga ttctgtgttt tatggccagt atgtgttaga
    10261 atatgaatat ttacatacgc aaatagattg tgctgtgctg ggctccggtg ttgcatcaac
    10321 attaaatgta gccttgggat tagataaccc ttctgtgcag atctttatgc ctcggagagg
    10381 aactaagggt atttctaata ttcagaatac ttttatacag cgcagtattc agcctatggg
    10441 tgtgtttcgg attcagcagg agttcgacag taaatatgtg attacctcgc ttgactttat
    10501 ccgcaacatg ctcgaatatc aaaacaatga aatcagtgca atcgaaataa gtgtaaaagg
    10561 cagggctgtc gacgatgtaa tggatgatat tgaatccatt atgggaaacg aatttaccgt
    10621 gaagaataga tttatgcaga atgaatttct ctatcgtgta atgcgcaccg agaaatgggc
    10681 tgtttatttt atcctcactt ttattttcat catcgcagcg tttaatatga ttgggtccat
    10741 agccatgttg gtgatcgata aaaagaaaga tattggtatt ttgagatcgt tgggcgcttc
    10801 tgaaaaatcg gtaagaaata ttttcttcat acagggaatt ttgcaaacac ttgtgagcat
    10861 tgctgtggga tttactttgg ccaccctgct ctgtttttta caaatgcatt tcggactaat
    10921 aaccattccc ggcgaaggaa cgtttgttgt tacggcttat ccgattgcat tagagtggcg
    10981 agattatgtg agcgtatttc ttaccgtgtt tattgttgga tgcatagctt cctggtttcc
    11041 ggcttatatg gctgcacgtc agaggtggct gttcaaaagt gaataaaaaa aagagcctcc
    11101 ggttggaagc tcttttatat agtgataagg tattatcaat aatcgccacc accattcact
    11161 tccgttaaag cggcatttac ataatccagt gccgattgta acgattgttt catctgtaaa
    11221 tcagtcgtgt tattgatcgc atcctgataa gtagttgcca tgctgcttaa ggtattcaca
    11281 gctgtaaacg acatccaaaa actgctttga tcggaagcaa cttttttcaa ttcgtcgaca
    11341 ccctttttaa tgatgtatga atctgtcatt cttaaaatga aaggctcata atacattaaa
    11401 acgtaataca cttcaaagcc actggcctct ttaaattttg ccaggaaata atcattgtcg
    11461 attgcttctc ccccatcgct gagaatgccc atgatgctat agctcaagtt atcattagct
    11521 tcagttttat atgtgcgagc caattcaaga ccttttttct tatcgttatt ataaattagg
    11581 gtcattgcgg tagcaactac attgtatgag ctgtcgttca tagctgcatt atacacgctc
    11641 catgcatcta taccggtgat ctcttttaat ttattcaaag ctgatgaacg cacgtaactc
    11701 cttggatcag atttagccat ttgcatgatc ttctctttta cagattgcgg agtgctggca
    11761 tcaataagca atgtatctat ggcaaattga cgaagcgacc aatgtttatc atcaagcgcc
    11821 gatgttataa ggtcgcggta acgcggatct tctgcctggt agggtgctaa agcttcgagt
    11881 gattcggctc tatcccgata taatggtgca tgttgatact ggaatatgta actgtcgata
    11941 tctttattgt catacttatt tccaagtagc attttttcag catcgacatt taccaaatcg
    12001 ggttgcacag gaaagttgaa tgaaaatgtt tgcatgctgc gatcaagtgt gatgcgtttg
    12061 cgctcaactt ttccattgta atagaagtct acatccattg gtattacata gacaggtgca
    12121 acggatgtgt cctggttttg ataaatggtg atatctaatt tatcctcttc agggaaccat
    12181 tcataatata tgcttaattc ggcatgcccc ttgtcgagaa accattcatt gaagaaccag
    12241 ctcaggtctt ctccactcac ttcttccatt gctttgcgca aatcgtggat ttcaacagac
    12301 tggaatttat ttttttcaag atataaattt aatcctgcaa agaaagcatc atcgccaaga
    12361 tatttacgca gcatgtgtaa tactcttcca ccttttgcat aagagtgaga gtcgaacatg
    12421 tcttcgcgtg tatcataatt atagcgaatc aggtcaacca ttttgtatga tgcctcattc
    12481 aaatacgttt gaaggtcatt atagccgata taatcagcat catcgcgacc atatttgtat
    12541 tcgcgccaga gatattctcc atatgtggcg aaagattcat tcaatggaat gttactccag
    12601 ctttcacagg taactaaatc gccaaaccaa tgatgaaata attcgtgaga aattacatct
    12661 tcatagtttt cgtcaagtaa ttcgcgcttg tgttgttgaa ggaattcgcc atgtaaggtt
    12721 gcgcttgcat tttccatggc accactcaca taatcacgca ctacaatctg gctatatttt
    12781 ggccaaggat aatttacacc taattttttt gaatagaagt cgatcatttc aggtgtattg
    12841 ccgaatatgg cgcgagcatc ttgttcataa tctttttcta catagtagtt tacctgcatt
    12901 ttgttccagg tatctttaac aatagcatat tcaccaacgg ccatcatgaa caaatatggt
    12961 gcgaatggta aatccatttt ccagttatcg gtgcgtgtgc catctgtatt tttcttcgaa
    13021 gaaatcagcg atccgttcga tagtgtttga tatttatcgg caactgtaat ggaaaaatct
    13081 gtggtgcaac gttcgtttgg tttatcgatg gtcgggcacc agcaagaagt ggcttctgtt
    13141 tctccttgag tccaaatttg catgggttta ttaggatcag ctccatcatt attgatgaag
    13201 tacaatccct tgtcacccgt aatggcaaca ctacctccca atggtaaatc atttggtttg
    13261 gcgatataat ctatatagat cataaagctt tcatcctttt tgaatgtctt accaagatca
    13321 atggtcattt gtaaggtgtc aggaattgta tctgtattat acacatattt cagcggtgtt
    13381 cttccggttg cagttaccag tgaaacttct ttaacggtaa atcctttggc atccagggtt
    13441 aatgttgaca ttggatggaa atatggcttt aatgtgagtg ttgctttgcc atacatccag
    13501 gctttcgacc agtcgaagga tacatctaat tttgtatgta acaaatcgaa ttcacgtttg
    13561 ctcgaacctc gatagatggc attgctatat ggactatatg ccgtatcatt ttgatagtcg
    13621 taactatagt cataatcata atcaccaacc gcatcggaat cgtaaccata atcataggca
    13681 tcatacgctg cagctcctga tgtatcgata gtacccaggt agggttcgga tttgtaaata
    13741 tgtttgtttg gatcgcactg atagaataaa gcagccgcta gtgccacggc tataaatggt
    13801 tgtaatttca tatctgtata ttacttggat cgaatggagt ccaaagttac ggattccatg
    13861 ctatctttaa cgccgaaaag cgctaatatc atgtacaacg taaccgtgaa cggaacacaa
    13921 gtttataaag ttgatggcaa tactgtcaac ggaaatgaaa ttctggctga tgttttaaaa
    13981 gtagataacc gattttacca tattttaaca ggaggaaagt catttaatgt ggaggtagtg
    14041 cgtcttgata agacagctaa aaccatgact ttgcaggtga atggcaccaa atacgaagta
    14101 tcgttacgcg ataaattcga tgctttgatg gaacaaatgg gttttggttc cggagcgggg
    14161 caaaaagctg cggatctaaa agcaccaatg ccgggactgg ttattgaggt gcgtgttcaa
    14221 cccggcgatc aggtaaaaaa aggcgacccg gtgattgtgc tcgaagccat gaaaatggaa
    14281 aacgtgctaa aagcacccgg tgatggtaag gttaaatcca tcgaagtgca gaaaggtgta
    14341 agcgttgaaa aaggacttgt gttgattaaa tttgaatgat cattatcatc cctgatatat
    14401 gtcatgtatg cggataagtc ccatacaggt tttcccttct actacaggca ataccgacaa
    14461 ttgagaactg cgattctcca tcaaatcaac tgcttctttt aatgtagcat gcacagaaac
    14521 acttaccgga tttcttgtca tgatactgga taccgacatg ctgcttaaat cggcatcgcg
    14581 cagcaaactt cttcttacat cgccatcagt aacaaggcca actaattctt gtgcatcatt
    14641 tacaacgcat gcagcgccca gattaaattt tgtcatagca agaattaatt cacgaaatga
    14701 atttttttct gatacacgtg caataaaagg catttcatgc atcacatctt caaccttgag
    14761 catgagattt tttcccaatt gcccaccggg atgaaatctc gcgaaatctt tttccgtaaa
    14821 tccccttgca tgcatcaagg caacagctaa agcatcaccc aatgcaagtg caacagttgt
    14881 tgatgcggtt ggcgccagat tgagggcgtc agcttctttt tcaactgaac catctaatac
    14941 tatggtcgac tgacgcgcaa taggtgaatt taaattacct acaatagcaa taaccgatga
    15001 ggatgattgc aggaaaaatg gaattaatcg aagcatctct tcagtggtgc cacttttaga
    15061 gatcatgaga ataggatcgc catcggcata tacacccaga tcgccatgtg ccgcttcagc
    15121 agcatgcaga aatacagcag gtgtgccggt agaacacaag gttgccgtga tcttttgagc
    15181 cactgctccg gattttccta ttccacaaac aacaacttta ccctttgatt gtaaaatggc
    15241 atttacggca gcatcaaaat tgtcatttaa atgatccagc gtgcggcgca actgctccga
    15301 ttcaaacaac aaaacatttt tggctatgtg taaatgtgac ataaacttgg ctgatttagc
    15361 acttatacaa aattacgaat taacgcgtga atttgcttca tagttgacat gctgtttatc
    15421 aaagatttga gtgactattg tcatggttta caaagcaatt ttgggccact atttaagaat
    15481 attcaattgt gcaagaaatt aaaaccttct tccttcaata tcgcgatata gatcttcttt
    15541 agataattgc tgaaagtatt ggtaggtaat ttgcaatcct tcgctgcgat gtaccttagg
    15601 ctcccaaccc agtatttttt tagcaagtga aatatcaggt tgacgttgct taggatcgtc
    15661 gacgggaagg gggtgatgca tgattttttg tgttgtacct gtgagtttaa tgatctcttc
    15721 ggcaaactca ttgatggtaa tttcatcagg attgccaagg tttaccggtt gcgcataatc
    15781 acacattaaa agacgataaa tcccttcaat aagatcacct acataacaaa atgatcgcgt
    15841 ttggctgcca tctccgaaaa cagtaaggtc ttctcctcgc aatgcttgtc caatgaatgc
    15901 cggcagcacg cgaccatcat ttaatcgcat acgaggtcca taggtattaa aaattctggc
    15961 gatacgtgtt tctaacccat gataggtatg ataggccatg gtaatagctt cctgaaagcg
    16021 cttcgcttca tcgtaaacac cgcgcggacc aacaggattc acatttcccc aataatcttc
    16081 actctgcgga tgcaccagtg gatcaccata aacttcactg gtagaggcga ccaatattct
    16141 ggcttgtttt gctttcgcca gtcctaataa attatgcgtt ccaagcgaac caacttttaa
    16201 tgtttgaatc gggattttta aataatcaat cggcgatgcc ggtgaggcga aatgtaatat
    16261 gtaatcaaga tcccccttta catgcacaaa ctgcgataca tcgtgatggt agaactcgaa
    16321 attggatagt tcaaataaat gttcaatatt tcttttgtcg ccggtgatga gattatccat
    16381 cgccatcacg tgcatgcctt ctgcaataaa tctatcacat aaatgcgatc ctaaaaatcc
    16441 ggctgcaccg gtgatgagga cacgaggttt gtggttgcta ttcattgatg ttaaagatag
    16501 aggttatgat ggagttgaaa gttgaatgtt ggaagttgag ggttgaaagt tgaatgttgg
    16561 aggttggaag ttgagggttg ttggttagtt gttattggtg ggttgggcct ttgcgggttg
    16621 ttgattggag atattgaata agactttgaa gcattccagt tattttttga gttttagcgc
    16681 agagcgtcac aaagtcatta tttgatatcc aatttctatc gttgcaacga tgaagttgag
    16741 aaattaattc tcctgaactt cctttcgcaa ttcgcagaaa ttggataaat tcttttgtgc
    16801 caccgcgtga aaatccttcg gcaatattat ccatgataga tccagatgaa cctctcattt
    16861 gccatcgcaa ttttgggtct tctgaaaatt cagctttttt tgtcaacagg taaatatctt
    16921 gagatacttt ccgagcctcc agccacacgg ccatctcctc aaaactctta aacgaaccca
    16981 aacagcaaca atttaattat gaaattaatc acccagcccc tcaattaata atcaaccaac
    17041 tacaaataac ctcacaaatc aaatcccaac tcccaaccct caaacccaca accttaaact
    17101 tacaaccctc aacttccaac ctcaaaccct cccaatacta taataaacaa tccccgcttg
    17161 cttcatttca gcaacttcaa aaacatttct accatcaaat atcacaggag atttaagtgc
    17221 tgattttatc gcttcgatat caggagtcct aaacacactc cactcggtaa caattataag
    17281 tgcatcagct ccggagagtg cttcgtattg attaacagca taattgatac gtttaccaat
    17341 ttgcttctct acattcggca tagcttcggg atcaaaagct gtaatggttg caccccgctc
    17401 aagcagcgca tcaattaaat ataatgccgg ggcttcgcga atgtcgtcag tatttggttt
    17461 aaaggctaga ccccacatgg caaaatgttt tccgctgagg tcgttcccaa aatgtgatgt
    17521 aaccttgtcg gctaaaatca cacgttgttt ttcatttaca gccataacgg aacgaagaat
    17581 atgaaaatca tatccattca caccggccat ttgatttaat gctttcacgt ctttcgggaa
    17641 acaacttcca ccatatccta caccgggaaa taaaaatcgt ttgccaatgc gactatcact
    17701 tccaatgcct ttgcgcacca tatctacatc ggcacctaat atctcacaca aattcgcaat
    17761 ctcattcatg aaagtaatct tggtagcaag gaatgcattg gctgcatatt ttgtcaactc
    17821 ggcgctgcgc tcatccataa aaattaccgg attgccctgg cgaacaaatg gtgcgtataa
    17881 tctgccgagc atttctttgg cgcgttcgct ttctgtgcct atcactacgc gttcaggttt
    17941 catgaaatct tctaccgcca ttccttcgcg caaaaattcc ggattcgaaa caacatcaaa
    18001 ttctgtagtg caatgtttcg caatcaatgc ccttaccata tcggctgtgc caacaggaac
    18061 cgtgctttta tcaataatta ttttatacga agtgataatt ttccccaaat cttccgctac
    18121 ctgcaacaca tatttcagat ctgcagatcc atcttcattt ggcggggttg gcaatgcaag
    18181 aaaaataata tctgttgcct caaccgcaga tttaagatcg gtggtaaatt gtaaattgcc
    18241 ttgtctgata tttctgtgaa aaattgtttc cagtccgggc tcgtaaatcg gaatctctcc
    18301 ctgttgcata cgttgcactt ttgccgcatc aatatcaacg caaatcacat gattccctgt
    18361 ctcagcaaaa caggtgcccg taaccagtcc aacataaccg gtgccgatca ctgaaatttt
    18421 cataccacaa aattaaggaa aacccctgta ttgagcgatt ttactatatt gctgcaagat
    18481 gctgaggtaa attgcttcgt atcaggcttc tgccttcatt ttttcagcat tttcagccac
    18541 ctgaagcgca ttgatgatct catctatctc accattgatc acttccggta ggttgtataa
    18601 ggttaaattg atgcgatggt cggtaacgcg actttgagga aaattatagg tgcgaatctt
    18661 ggcagatcgg tcgcctgtag aaaccaaggt cttacgctgt ttcgatattt tctcaaggtg
    18721 ctcgttatga aatttttcat acacacgtgt gcgcaacacc ttcatggctt tttctctatt
    18781 cttaatttga gagcgttcat cctgacactc ggcaacaatc cccgttggga tatgtgtcat
    18841 acgcacagca ctctcagttc tgtttacgtg ctgaccaccg gcgccactcg ctctgaaaac
    18901 atctactttc acgtcggtag cattgatctc tacatcaaac tcttctgcct caggtagcac
    18961 agcaactgta gctgcactgg tatgcacgcg tccctgtgat tctgtttccg gaacacgctg
    19021 taccctgtga acacctgatt catactttaa tctgccataa acattttcgc cacttacctc
    19081 taacacaatt tctttatatc caccctgatc actcgcattt tcattcatga tctctattcg
    19141 ccacccttgt ttgccgcaaa atcttgtgta catctcactc agatcgcggg caaataatgc
    19201 cgcttcatca ccacctgttc cggcgcgaat ttcaatgatg gcattttttt catcctcggg
    19261 atctttcggg attaataatt gtctgatttc ttcttcaagt gttgctttct cggtttcata
    19321 cgctgcaatt tcttccttcg ccatatcgcg caattccgca tcgcgctctg tatttaaaat
    19381 agtgcgactg tcatccaaat tcgacaacac cagttgatat cgctcatacg ctgcaacaac
    19441 aggttccata tcgcgatatg atttattcag cgatttaaaa cgcttcatat ctgcaataat
    19501 atccggatcg cttagctgtt cctgaatatt cagccagcgc tccttgattg cttgtaattt
    19561 ccctgtcaga tcgatttgat catgcatgat gatgcaaaat taattattta attgtcaata
    19621 atttatgaat ggtaatggtg ctatccactc aattaatccg gctacctgaa ttgcaataca
    19681 ttaatatgat cgccatcata ataatacatc aaatcatttt gtaatctgac atcgccggtt
    19741 gcaggtagtg gtaaggcaat aacttcattg gtgaagatat tcacaagaga gaatcctgtt
    19801 ggtcctttta tgagaaacat atttctatat gccgaaaaag gtgcatcgtt gagcataaat
    19861 gttgtaccgg gagtgccata tgcatcgaac aaatatatta atccgccact agtatacaca
    19921 tacaaatggt cttgtatgtc aaccatccaa agggggactt ccgatttttc aaatatctga
    19981 tatattggtt cgctggcggc aattttgttt ccactctcat ccaatctgac gaatgattgt
    20041 gtaaattcat caaacaacca aatatgatca cctcctgttg cacggcaaag taatgagggc
    20101 atatatgatt taccattgat gttgttgaat gataacacag caatttccga catggtttta
    20161 tctaacacta ccaccttttg ctgatctttg taaaatgcca gtatcttcag cgggttgcca
    20221 acatctatca aagttggagt cccattggca atattgctgt aggtgatggt atggttgcca
    20281 caggtatcgg tgcggctgat ttcatttgaa gcagtgacat aatatagcat gccatttgca
    20341 tctaccgtaa aataacttgg ttttacggca atagtgcata ccagcgaatc agtagcaccc
    20401 aactgcaatt gcagcataca gatgatacct aaaatactaa acttcaaaat attttatttt
    20461 gcaggtttga ccgtcaaatt ctgcaaaaga ataatgacgc acccaatcgc ctaagttaat
    20521 aaatgtactg ttgtcactca attttaattc taaaacgata tgccgatggc caaaaaggaa
    20581 atagtcgata tgctcttttt gaagcatctc ctttgcataa attatctgcc attctttttc
    20641 ctcgccaaga aatgtttcct cttcatttaa cattctgctg ttgtgcgaga aataacgcgc
    20701 aagtttgaat gctaagtatg gatgtaacag actgtataaa aattgtgcaa agcgattcgt
    20761 gaaaactgat ttcattaatt taaagccgcg atcaccgggg ccaacaccat cgccgtgtcc
    20821 gatataaaat ttttttccat gtgattctat gaaaatcgga tgatggtgca cagggatatt
    20881 cagttcttct tcaaaatatc caaacatcca caaatcgtga ttgccggtaa acgcttgtat
    20941 gtcgatacca ctatcacgta attcggcaag cttgcctaat aagcgtacat aacctcgtgg
    21001 aacaacatga tcatattcaa accaaaaatc gaaagtatcg cccaataaaa agatctgctt
    21061 ggcatcatgt ttaatagaat ctaaccagcg cacgattttt ttctcccttt ccagacttac
    21121 tgaatgattg gggatgccta aatgaaaatc ggaagcaaaa tatatcttag ttctttccat
    21181 cccatccggc cattggccag ttatcaatca gaaatacata tacttctctg ggaccatgtg
    21241 caccaagcac aagtgttttt tcaatatcgg cggtgcggct tggaccggta ttcagattca
    21301 ccatacttgg gaagtttccg tcataatgtt tgcgtatggc agcaaagcca tcctgaatat
    21361 ccaataccaa ttgtttggtg ctggcgatca cgatgttcac ctcgggaaag ataggcaagg
    21421 tgcgcccgct ggcctggcga ctactgatga ggatagtgcc tgttcgggct attagcgctt
    21481 cgcaaagcat aatacctgca tctgcgccgg ttaccggagc atcggcatcg tatcttatga
    21541 attgctgacg gtcgaaaagc tcatgcagca agggttccgg gcaaacaata tgttcccatt
    21601 tcttgtgggc aacgagctcc ttgagggtgt ctacacattc cttttcggtc tcgcaaaaaa
    21661 caaattgacc tgaaacagct gtaaactcgg tagcaaaaat aatctccaac ggttcgtcag
    21721 gaacattgta gatattgtcg ttgctttcca gattcggata tggctggcga gtttgatgca
    21781 agagtgctcc acgcacactg cttagcattt tttctttgga actaaggatc ttggccatgg
    21841 attcgctgtt gctgtaaagt tagggaaaag gatcgaaatt tattacaaac gcaacagcat
    21901 agccaagggt cacagcgagc catagacccg ctttgtagta cacttggcaa taccccaggc
    21961 agacatttgc aaattcaatc atgtcatgta tctgatatga catgaatgcc aatagcatta
    22021 atgtatgcca aactttgact aaaacaagga tcgattaaaa tcgcagatct accaattata
    22081 aagtttgg
//
LOCUS       DAOQOU010000038        21939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1139_length_21939_cov_6.255849, whole genome shotgun sequence.
ACCESSION   DAOQOU010000038 DAOQOU010000000
VERSION     DAOQOU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21939
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1139_length_21939_cov_6.255849"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLM90_05215"
     CDS             complement(<1..875)
                     /locus_tag="PLM90_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88801.1"
                     /translation="MKLNYLIMLAATGLMAASCATQTASRGASSDDIYYSAGDPTYTE
                     ESIKTETTTSSSNYSTYPGTSAQTEYAYSEPQDDAGYRMGGDDLSSSNEYYDDEGNTY
                     IVNNYYDDYDYDDYYYTSSLYRFYSPYYGFGYHSHCYSPYYYDPFWGPSYGWNVGYSW
                     GWGGIYYGSYYPYYPYNYGYCYNPYGYYGYYDPWYNPYYGYGGYYNGYNNGYYNGYWN
                     GYYDGYYDNGYYSGNYGGYYGNSDDYYYGVNTGTGSNTNTGDPSANILSAGYDKAGNG
                     TTDMNHILTDKGSLVV"
     gene            967..2442
                     /gene="proS"
                     /locus_tag="PLM90_05220"
     CDS             967..2442
                     /gene="proS"
                     /locus_tag="PLM90_05220"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMZ88802.1"
                     /translation="MAEKITKRGVNYSEWYNDLVIQSGLAEYAAVRGCMVIKPYGWAI
                     WENMKTVLDTKFKETGHVNASFPLLIPKGFLEQEEGHAEGFAKECAVVTHYRLKTNPD
                     KKGKLMVDPESRLEEELIIRPTSETIIWNCYKGWIQSYRDLPILINQWANVMRWEMRT
                     RLFLRTAEFHWQEGHTAHATSEEAVEETVKMLNVYADFMENYMAVPVIKGVKTANERF
                     AGAVDTYCVEGLMQDGKALQCGTSHFLGQNFAKAFDVKFLTREGKQEYAWATSWGVST
                     RMMGALIMAHSDDEGLVIPPRLAPLHVVIVPIYKTEEQLAAITAIAEGIMKELKDLKI
                     TVKYDDNDQAKPGWKFAEYELKGVPVRIAMGGRDVENGTVEIARRDTKEKVTVKNENL
                     GLYIQQLLEDIQQNLYNRALQFRTASTHEVDSWDDFKKQIEIGGFVSAHWDGTSETEE
                     KIKEETKATIRCIPLDAVQETGNCVYSGKPSTQRVLFARAY"
     gene            2700..5636
                     /locus_tag="PLM90_05225"
     CDS             2700..5636
                     /locus_tag="PLM90_05225"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ88803.1"
                     /translation="MKAGFRQLKRTKQLVVKELLYALLFVVLTQWHLSLVGQHPYIVP
                     VTPADDYLPTNSFYVFRDNSDVMWIATNSGVYRYDGYAFTHFTSADGLGDNEILRIYQ
                     DKKNRIWFQPINGNPSFYLNGSIHNAQNDSLVKQLEFNKMVLAECEDDRGNLYLAGRG
                     PVVYKINDRDEVSQISVSGLENFIWVAKDGSLKMMDKASFPTKIIRADTYGSMHLISN
                     EQDVYSLKDDNILEKEFTLPQGSAEIIFIKFINYEEVFIGTRNGLFIWNRITKDYKYL
                     MPGYSVSSAERDFEGNTWISTLQSGVYLMPNVNVSFWNSDYGLSEDKITALEADADGR
                     LWIGCANNHYAIMENGKIIADRRLPEPTPMDIMNIRHFGDRTYVVGKAGILGMGKGFN
                     KSYGVYANDLIFYNDMVMIGQDNAMLFTRDEFETRIKQLVFNYPERKRDYEVINARTN
                     VFCQINNAIWIGTSRGLYIYENGKTSFVGDHIAACKNPIRDILFDSRTGLQFVGTMNG
                     LLVLRDEQLVTIIDKNSGLPNSDCNAIFLDKEGIIWAAFGNQLVAITKNGENFTTINY
                     TKALRLDPSRITDVHVAQGKVFISTESGLIAFDQNIELPNPVPPRIIISSFQVNGKES
                     ELGKDVRLHWKQNDITVQYSGMSFTSKNRITYTYYLSGYDTKWHNTTDRSIQLKSLPP
                     GDYKFLVKATNIAGISSPVSTISFSINKPVWGEVWFIILSVCALAGIILIGWRMRLRA
                     VKQSFTVKQRTIQLEMEHAEAERKIEQLNQQVFRQQMNPHFIFNALNTIKGYYAENDI
                     KKASDYISKFSKLLRNILENNEQWIPIEREINAITLYLDLAAMRYAYKFTYNISAHQP
                     LQVMRTGIPPMLLQPFIENALVHGIAPKQGSGHIAIDFSVSDNKLICIVEDDGIGRKA
                     SATRSRISEHNSKATQIINDYLQALNQRERAEAFILDIDDLTDETGKGIGTRIRLSMP
                     LIILNP"
     gene            5648..6400
                     /locus_tag="PLM90_05230"
     CDS             5648..6400
                     /locus_tag="PLM90_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ88804.1"
                     /translation="MLKAIIIDDESRARSALKQEITLNCPDVHIIGEADSISASVSLL
                     EKADPDIIFLDIQLSDGLGFTVLEQIENDDYAIIFTTAYSEYALKAFKTSAVDYLLKP
                     IDSEELVAAVNKVKNAKEEKHLQQVANSARRDLNALQQKKIILHTTEGLHVIPIGDII
                     RCNSYGNYSFVFLKDGSKILLAKILKEIEESLQHYGFERVHHSHLINLNHVKSFRNKD
                     GGMVMMSDMSEVPVSMRKKSAFLDELEKLNSL"
     gene            6828..8726
                     /locus_tag="PLM90_05235"
     CDS             6828..8726
                     /locus_tag="PLM90_05235"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ88805.1"
                     /translation="MFNQFQLKGLLLLLLVCSSLLTKAQHDCSCASYNKSFRDQYEAL
                     FSMRKLDSARLLSNTLIKQKGCCEAAGNILVANTFLIDKQFDSIGKFVDRAEKLIGNN
                     YDTLVSPEILRLRGAVAAGKADLDKAASYYAEGIEQSKKHQNLKYAARFCNELSHTYG
                     SMNKPEPSLKFLKEGAAIAEQIKDDKLCAMLYANILATYGMFHENTHNKAYLDSMEMY
                     APISIKYAQLSKDPYYIVRGYTSLGGIAIEKGDYKTALAYNDTVMAMLPPAGAEQLQI
                     SAQYRRGQCLLQLGRVEEALASLHAALGNAKKFKSQGIVSLINQQLYFTYRDLGKSDS
                     ALYYYEQHVAVRDSLVNIENTSALTELEQKYDKVQNEKKISELSKEKQISTLQIRILA
                     AGILVTLLVSLSIFLYYRQKTLNNKKLILETEQRLNRARMNPHFFFNALASLQGFALR
                     ENDGKALASNLSKFSHIMRETLESTYKDYVTIQQEIDFLYEYLTLQQIRFPQAFKFVM
                     SADALSEPDSLLIPSMIIQPFVENSIEHGFRGIDYVGEITILFDEDGDNIVVSILDNG
                     KGLAGEKEITNEHISRASQIIRDRIYLLNLKLKTNASFQIANQRDGKGVQVVIRLPKI
                     TEDESLNN"
     gene            8707..9447
                     /locus_tag="PLM90_05240"
     CDS             8707..9447
                     /locus_tag="PLM90_05240"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ88806.1"
                     /translation="MKVLIIDNETPIRVALHEMLLAYCPEITHIKEASDIKTGIDTIG
                     WFNPDILFLDVELDEGTGFDLLSQLPAYTFQLIFITAHDKYAINAFRFSAMDFLLKPI
                     DPDDLMHSVGRAIKNIQSDPSKQMAFLLEQVKSKHEPPKKIILKDMHKTYYVRIEEIL
                     FLEAEGIYTKFNLMGNQQIMVSKNLKEYESMLEPLGFVRTHHSFLVNPEKIKMFDKSE
                     GGMLVLEDGNQIPVSQRKRDLVLKALEH"
     gene            9529..9963
                     /locus_tag="PLM90_05245"
     CDS             9529..9963
                     /locus_tag="PLM90_05245"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ88807.1"
                     /translation="MYYTYQITINKPVDEVVRLFDNPDNLYKWMSGLQSFELISGTAG
                     EPGAKSKLVFLNGKRRMEMIETITVRDLPAEFSGTYEAQGVVNISVNKFKDLGNGTTL
                     YTTENTFHFSGFMKLIGFMMPGAFKKQSYKYMEQFKAFVESH"
     gene            complement(10027..11433)
                     /locus_tag="PLM90_05250"
     CDS             complement(10027..11433)
                     /locus_tag="PLM90_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88808.1"
                     /translation="MRTLKHLFLTGILLPLSFAYAKACDYSHFDLVSTTDLGGGQYEF
                     VVTFCAGGGEGGADQSTYTWGVELVGPATFSSWPATLASPQTGAIYAADGSFYYGSNY
                     LIYFLDSYTGTGFAAQSWTTASAGYGPAGSYCKTFTIVTNGMPTQLRLMGAEAAGIAV
                     EPYGCNGAPDMEINFDVAADAGLTTSLCAGNTVTLNATATGGTPPYSFVWNTGATTAS
                     INVSPTVNTTYTVTVTDANGHTDSDPVVVVVHPKPTVNAGTDKTITIGYGATCATLTG
                     TATGGSSPYTYSWTGGGTSLTKTVCPTTTTTYTLTATDYYGCSNSDAALVTVKDVRCG
                     PSLTKVYVCKMGTTKCITTGQVPSYLTSGWVLGACWMKLDDEVALSENPIDIFPNPAT
                     TSAEIVFVMTEDGEATIELYSLNGERFPIADAVVAGRAGEEISHVIALDNIPAGIYQI
                     FVHTTSGQIMTEKLAVIR"
     gene            complement(11561..12808)
                     /locus_tag="PLM90_05255"
     CDS             complement(11561..12808)
                     /locus_tag="PLM90_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ88809.1"
                     /translation="MNNNQRRTTIFNLTVLVAALGYFVDIYDLQLFNLVSKTSLKGIG
                     ITDPSLVDYWDVRLFNVQMIGMLVGGIIWGIMGDLKGRKSILFGSILLYSAANILNGF
                     VDNTTQYQVVRFFAGLGLAGELGAAITLVAEIMSKEHRGYGTMLIVSVGALGAVAAFF
                     ITSHVDWRVSYYIGGGLGLALLLLRFGTFESTMFDEVKQQNVARGQFLSLFSDRKRFL
                     KYVYCILIGIPVWYCIGVLMKFSERFAKEWGIEMTPEEAIQVRRTAIMLSYIGLCVGD
                     LLSGYLSQVFKSRKKIVMGYLIATVVLALVYLNMKSSSLTLFHIMCFLLGCATGYWAL
                     FVTIASEQFGTNIRATVTTTVPNFVRGLVVPLTLSFAGLSVSVGNIQSAMVVGAVSIG
                     IAMIATLSIKETFGKDLNYMELS"
     gene            complement(12826..13323)
                     /locus_tag="PLM90_05260"
     CDS             complement(12826..13323)
                     /locus_tag="PLM90_05260"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMZ88810.1"
                     /translation="MPLFSIIVAASENEVIGMQGDMPWLKLTSDLKYFKEKTSGHWCI
                     LGRKTYNALGNKVLPNRKFIIVTRDRNFKSDDSIVVHDLNEARHHPLIQQEEEVFILG
                     GGDIYRQAMSFVDRIYLTRIHASFEGDTYFATPGEGFHLASKDVRTIDEKNPYDHDFL
                     VYEKN"
     gene            complement(13333..14127)
                     /locus_tag="PLM90_05265"
     CDS             complement(13333..14127)
                     /locus_tag="PLM90_05265"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HMZ88811.1"
                     /translation="MRQYHALLQRILDEGTRKSDRTGTGTVSIFGHQMRFDLQEGFPL
                     VTTKKLHLRSIIHELLWFLKGDGNIAYLKENGVSIWDEWADEHGNLGPVYGVQWRSWP
                     TADGRHIDQISNLINQIKKSPDSRRLIVSAWNVADIENMALPPCHCMFQFYVADGKLS
                     CQLYQRSADTFLGVPFNIASYALLTMMVAQVCDLQYGEFIHTFGDAHLYNDHIEQAKL
                     QLSREPRVLPQMKINPDVKDIFGFKFEDFELVNYDPHPHIKAKVSV"
     gene            complement(14244..15977)
                     /locus_tag="PLM90_05270"
     CDS             complement(14244..15977)
                     /locus_tag="PLM90_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ88812.1"
                     /translation="MSTIDWLVMFGTLLLIAVYGIYRTRQIHTAETYLRGDNEKRWWE
                     IGLSVMATQASAITFLSVPGQAYDNGLGFIQFYLGLPLAMIVISVFFIPIFYRLKVYT
                     AYEFLENRFDVKSRTLTAVLFLVQRAFSTGITIYAPSIILSQLLGWPLTSTVIFIGGI
                     VTLYILSGGNKAVTVTHKQQMLVIYAGMFTAFGFLVYYITRDINLGEGISLAGYMGKM
                     HVITTGSESGHFDWSDRYNIVSGLIGGLFLQLSYFGTDQSQVGRYIGGASVRESRLGL
                     LFNGLVKIPVQFFILFIGLLVFTFYQLNTPPVYFNDVAYQKALHSDYGPQLEKLASDY
                     QLASELQQSNARQTVNRKEQTIYEYQESGRKADSIRLEVQKIVEKVEPSTQDDHDYIF
                     MRFVMDHLPVGVIGLLFAVIFCASMSSTASALNGLSTTFSVDIYKRFMFKNATGKQHL
                     QASKMLTAAFGILAIMFALIVSMFDNLIEAVNILGSLFYGTILGIFLTAFLLKRVGGN
                     AVFVAAVISELVILYIDFSTRYHWNTPTIQIGYLWYNVIGCLLVMGISLVLSFILPKY
                     EIISEGIDLPE"
     gene            complement(16081..18624)
                     /locus_tag="PLM90_05275"
     CDS             complement(16081..18624)
                     /locus_tag="PLM90_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HMZ88813.1"
                     /translation="MFLMQAMNLHAQNPSWSASEIQLEIEKLTSSHSVLYIAAHPDDE
                     NTRLITWLANEKTVRTGYLSLTRGDGGQNLVGEEQGAYLGVIRTQELLAARRMDGGEQ
                     FFTRAVDFGYSKSASETFTKWPHDSLLADVVWVIRNFQPDVIITRFPTDERAGHGHHT
                     ASAMLAEEAFDAAADPTKFPDQLKYVSVWQAKRLFWNNSTWWDKQLPEKIAKADPTLA
                     VIDVGGYNAFLGKSYGEIAAESRTNHKSQGFGSAPTRGEQKEYLELKKGEAFSNNDIF
                     SGLPIDWSVYENKVSALALASSAFQNFDAKHPDRSIPILIDYIKTLETFPQDPIVIRR
                     IQQAQQIIGACIGLFLEPAATTEKVAAGESLTIVSTAVKRLDYPITLESIEILGKTYK
                     EGEILPTNINQIDTIIFEVPEGITSSPYWLENDYNTLFDVRDRSLIGKAENDPAISVI
                     YHVKIGDRSFAFKRGVIYKETDAVKGEIIQPLTVLPKALIRANKSLLMFRYNEPLFVD
                     VIVESNIKDLDGFVFVSNDEGFIVSGGREIRTLQAGQTIAVQFSIQAPTNSKVETGNL
                     KLYFIPGKNAKAIYDSLIHSGVYHAVENYIVGSKKGVYRDLQTISYDHIPIQQILEHA
                     EVRMVRIQSPVPTFPVAYIEGAGDKVDESLRAFGVQVTTINPEDITQALLQQFPVVII
                     GVRAYNTSKAMAEKQALLMDYVQAGGTLITQYSTNWDSYVDQLGPYPFKLTRNRVTDE
                     QSPVSFLLPDNPALNTPNKITENDFKGWVQERGIYFVGEADSSYAFPLGLTDPGEKEQ
                     NGALMVAHYGNGVFIYTGLVFFRELPAGVPGAYRLFVNLMSMRGKSQPK"
     gene            complement(18936..19310)
                     /locus_tag="PLM90_05280"
     CDS             complement(18936..19310)
                     /locus_tag="PLM90_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88814.1"
                     /translation="MRKLLTILFALVLSGHFASAATADGEDLIRKATAVIDETVQHYA
                     AKEGEKKVSVSTILPDPDKQNVIITFTIDELNRIHVVEIQGGYAYLTHYIKSSLEGQT
                     IKSEHAIPGINYMMAIKLPASA"
     gene            19500..20024
                     /locus_tag="PLM90_05285"
     CDS             19500..20024
                     /locus_tag="PLM90_05285"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88815.1"
                     /translation="MPHQEPISIRLALSADAPLLAKLGAKTFFDTYQAFNTPDNMTDY
                     ISKNFNINALEAELGDEAVSFFIAFSGDSGVGYIKLSQGVSSYVSENPAMEVSRYYVD
                     AGFQGRKVGAALMQSAFEHAKANNCKVIWLAVWQKNPKAVNIYMHQGFEIIGTTTFQL
                     GDDLQDDYVMAKRL"
     gene            20025..20729
                     /locus_tag="PLM90_05290"
     CDS             20025..20729
                     /locus_tag="PLM90_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HMZ88816.1"
                     /translation="MFLSIIIPTFNEAGFIGNTLEFLLKQTYDMPFEIIVADGGSTDD
                     TTSIASNFPVSVLQSPERGRAQQMNFAARFAKGTILFFVHADCLPPANFSSFIRNALN
                     KGVDAGCFRYRFDSNKRMLQFNSFFNRFGGLLFRGGDQTLFIKKDIFSALGGFDESYI
                     VMEDYDMVRRISKGHRFEVLRDEAMVSARKYDENSWLKVNIANAVAMSMYLTGLFCPL
                     TIRNTYHRMIKHPKGR"
     gene            20744..21541
                     /locus_tag="PLM90_05295"
     CDS             20744..21541
                     /locus_tag="PLM90_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ88817.1"
                     /translation="MFAGKVIWITGASSGIGEELAYQLATKKAYLILSARRTLELERV
                     REQCLVHTNCVDILSLDLSDNNALPEMAKRAEAIHGRIDVIIHSGGISQRDSMLNTVP
                     DAEARIMQINFTSVVTITRALLPGMIARGEGRIVLLSSVAGKIGVPNRTTYCASKHAI
                     IGYFDALRAELKIAKQPINIHIMVPGFIHTNISVNAVLGDGNPQGVMDPGQEKGMPVE
                     KCVQQMIRGMEKNKKEILIGGKEILMTYFRRFIPGLYYYLITRVKTK"
     gene            21562..>21939
                     /locus_tag="PLM90_05300"
     CDS             21562..>21939
                     /locus_tag="PLM90_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88818.1"
                     /translation="MSDKPIISGIQQVGIGNPYVHEAWRWYRENFGMDVKIFEEAAEA
                     NLMLPYTGGMPHKRHAVLAINMNGGGGFEIWQYTSRKPVAPAFQVQYGDLGIYARKMK
                     SRDVQATYNFLKNRGINLLGDVTN"
BASE COUNT         6888 a         4400 c         4502 g         6149 t
ORIGIN      
        1 accaccagtg atcctttatc ggtaaggata tggttcatat cagtagtacc attgccggct
       61 ttatcataac ctgcggaaag gatgtttgca gaaggatcgc cggtattggt atttgaaccg
      121 gttcctgtgt ttacaccgta gtaataatcg tcgctgtttc cgtagtagcc accatagttt
      181 ccactgtaat agccattatc ataatagcca tcatagtaac cattccagta accgttgtaa
      241 tatccgttgt tatatccgtt atagtatccg ccataaccgt agtaaggatt ataccatgga
      301 tcgtaatagc cataatatcc ataagggttg tagcaataac cgtaattata aggataataa
      361 ggatagtagc tgccataata aatgccgccc cagccccagc tataacctac attccaacca
      421 taagatggtc cccagaaagg atcataatag tatggagaat agcaatgtga gtggtaacca
      481 aatccgtaat acggagaata aaaacgataa agactagatg tataatagta atcatcatag
      541 tcataatcgt cgtaatagtt atttacgatg taagtgtttc cttcatcatc ataatactca
      601 tttgaagaag acaggtcatc gccacccatg cgatagccgg catcgtcctg tggttctgaa
      661 taagcatatt cagtttgggc ggaagtgccg ggataggtgg agtaattaga acttgaagtg
      721 gtggtttcgg ttttaatgct ctcctcagtg taggtaggat cacctgctga gtagtagata
      781 tcgtcagatg aagcacctct agatgctgtc tgtgtggcgc aggatgcagc catcaatccg
      841 gtggcggcca gcatgatcag gtagttcagt ttcataatcg atattttaaa aggtttatag
      901 aatgctgcaa agggaatctt tacctttgcg aatcatataa aacataaatc ataccaaaaa
      961 tacaatatgg ccgaaaagat tacaaaaaga ggtgttaatt attccgagtg gtataatgat
     1021 ttggttatcc aaagtggcct ggcagagtat gctgccgtga ggggttgtat ggttataaag
     1081 ccctatggat gggcgatttg ggagaatatg aagaccgttt tggacacaaa atttaaggaa
     1141 acaggtcacg tgaatgccag ttttccactt ttgataccga aaggcttcct tgaacaagaa
     1201 gagggacatg ctgaaggttt tgccaaagaa tgtgccgtag taacgcatta tcgcctgaaa
     1261 accaatcccg ataagaaagg caagctgatg gtggaccctg aaagccgcct ggaagaggaa
     1321 cttatcatca ggcctacctc tgaaacgatc atctggaact gctataaagg ctggattcag
     1381 agctatcgcg acctgcctat cctcatcaac caatgggcaa atgttatgcg ttgggaaatg
     1441 aggacccgtc tgtttttgag gactgccgag tttcattggc aggaaggcca tacagctcat
     1501 gctacaagcg aggaagcggt tgaggaaacc gtgaaaatgc tcaatgtgta tgctgatttc
     1561 atggaaaact acatggcagt tcctgttatc aagggtgtga aaacggcaaa tgagcgcttt
     1621 gcaggagccg tagatacata ttgtgtagaa ggcttgatgc aggatggtaa agcgttgcaa
     1681 tgtggtactt cgcacttttt gggacaaaat tttgcgaaag catttgatgt gaaattttta
     1741 acgcgcgaag gaaaacagga atacgcatgg gccaccagtt ggggtgtatc aacgcgtatg
     1801 atgggtgcac tcatcatggc acatagcgat gatgaaggtt tagtgattcc acctcgcctg
     1861 gctcctttgc atgtggtgat agtgccaatt tataaaactg aagaacaact cgccgctatt
     1921 actgccatcg ccgaaggcat catgaaggaa ttaaaagatc taaagatcac cgtgaaatac
     1981 gatgacaatg atcaggcaaa gccgggatgg aaattcgctg aatacgaatt gaaaggtgtt
     2041 cctgtgcgca tagccatggg tggtcgcgat gtagaaaatg gtacggtgga aattgcacgt
     2101 cgcgatacca aggaaaaggt gacagtgaag aatgaaaatc ttggtttata tattcaacaa
     2161 ttgttggaag atattcaaca gaacttatat aaccgcgcat tgcaattccg caccgcaagc
     2221 acgcatgaag ttgacagttg ggatgatttc aagaaacaaa tcgaaatcgg tggttttgta
     2281 agtgcgcatt gggacggcac ttcagaaacg gaagaaaaga tcaaggaaga aactaaggca
     2341 actattcgtt gtatccccct ggatgccgtg caggaaaccg gaaattgtgt gtattccgga
     2401 aaaccaagca cacagcgcgt attatttgca agagcatatt aatcaatcaa ccatatactt
     2461 ctaaagcccg atagcatgta tcgggctttt ttgtgtccga taatctgcat tgattgccat
     2521 tgtcgaaaat tagctagaaa tatcaaagtg ataacatttt agcgacaact gcaataaccc
     2581 ggcatataag tgttgacttt taccgaataa gtgatttgcc aaagggtaat aactgaaata
     2641 tttgcagggg gtaatatgac agtattctct tatttttata gcctgcaata gttataacat
     2701 tgaaagcagg gttccgacaa ttaaaacgca caaagcagtt agtggtaaaa gagctgttgt
     2761 atgcactatt gtttgtagtg ttgacgcaat ggcatttatc gctagttggg caacatccgt
     2821 atatcgttcc tgtaacacct gctgatgatt atttaccgac caactcattt tatgttttca
     2881 gagataactc tgatgtaatg tggatagcca caaatagtgg tgtttatcgt tatgacggat
     2941 atgcgtttac ccatttcacc agtgccgatg ggctaggtga taatgaaatt ttgcgcattt
     3001 atcaggataa aaagaataga atttggttcc agcctatcaa tggaaatcca tcattttatt
     3061 tgaatggcag cattcacaat gcacaaaacg attcgctggt aaagcaattg gaattcaaca
     3121 aaatggtttt ggccgaatgt gaagatgaca gaggtaattt atatttggca ggaaggggtc
     3181 cggtagtgta taaaataaat gaccgtgatg aagtttcaca gatatcggtg agcggacttg
     3241 agaatttcat ctgggtagca aaggacggct cgctcaagat gatggataaa gcttcctttc
     3301 caacaaaaat aattcgggca gatacatacg gaagtatgca tttgatcagt aatgagcaag
     3361 acgtatacag tctgaaagat gacaacattc ttgaaaagga gtttacgttg ccgcaaggaa
     3421 gtgccgaaat aatatttatt aaattcatca actatgaaga agtatttata ggcacgcgca
     3481 acggattatt catttggaac aggattacaa aagattacaa atatctaatg ccgggctatt
     3541 ctgtatcatc ggcagaaaga gatttcgaag gcaacacctg gataagcaca ttacaaagcg
     3601 gtgtatacct gatgccaaat gtaaatgtct ccttttggaa tagcgattac ggtctttctg
     3661 aagataaaat aacggcattg gaagcggatg cagacggtcg attatggata ggttgtgcca
     3721 ataaccatta tgccattatg gaaaatggaa aaataattgc agataggagg ttaccggaac
     3781 cgacgcctat ggatatcatg aatattcgac attttggtga tagaacatat gtagttggca
     3841 aagcaggaat tttgggtatg ggcaaaggat ttaataaatc atatggtgta tatgccaatg
     3901 acctcatttt ttataatgac atggtcatga ttgggcagga taatgctatg ctttttacaa
     3961 gagatgagtt tgaaacaaga ataaaacaat tggtttttaa ctaccctgag cgaaaaagag
     4021 attatgaagt aatcaatgca agaacaaatg tattttgtca aatcaacaat gccatttgga
     4081 taggcacttc cagagggctt tacatttatg aaaatggtaa aaccagcttt gtaggcgatc
     4141 acattgctgc gtgtaaaaac ccgatccgag acattctttt tgattctaga accggattgc
     4201 agtttgtggg taccatgaat ggtttattgg ttttacgcga cgagcaatta gtaaccataa
     4261 tagataaaaa ctccggctta cctaatagcg actgtaatgc catatttctt gacaaggagg
     4321 gaataatttg ggctgccttt ggaaatcaat tagttgccat cacaaaaaac ggtgaaaatt
     4381 tcacaactat taactatact aaggctttgc gccttgaccc ttccaggatc acagatgttc
     4441 atgtagcaca agggaaagta ttcatttcta cagaaagcgg attaattgcc tttgatcaaa
     4501 acatagagtt gccaaatcct gtgccgccga gaataataat cagctctttt caggttaacg
     4561 gcaaagagtc tgaacttggg aaagatgtta gattgcattg gaaacaaaat gacattactg
     4621 tacaatattc cgggatgtca tttacgagta aaaaccgcat tacttacact tactatttaa
     4681 gtggttatga tacaaaatgg cacaacacta cagatagaag cattcaatta aaatcccttc
     4741 ctccgggaga ttacaaattc cttgtaaagg ctaccaatat tgcaggaatt tctagtcctg
     4801 taagcacaat atctttttcc ataaacaaac cggtttgggg ggaggtgtgg tttatcattt
     4861 taagtgtttg tgcacttgca ggaattattt taatcggatg gcgaatgcgt cttcgtgcag
     4921 taaaacaatc ctttacagtg aagcagcgta ctattcaact ggaaatggaa catgcagaag
     4981 cggagcggaa aattgaacag ttgaatcaac aggtattcag gcaacaaatg aacccgcatt
     5041 ttattttcaa tgccctgaat acgatcaaag ggtattatgc agaaaatgat atcaagaaag
     5101 ccagcgatta tatctctaaa ttctctaaat tattacggaa tattctagaa aataatgagc
     5161 agtggatacc gattgagcga gaaattaatg caataacact ctatctcgat ctggctgcaa
     5221 tgcgttatgc atataagttt acttacaaca tcagcgcgca tcaaccacta caagttatgc
     5281 ggaccggcat tccaccaatg ctgcttcaac catttattga gaatgcactt gttcacggta
     5341 tagcacccaa gcaaggcagt ggacatatcg ccattgactt cagtgtttcc gataataaat
     5401 tgatctgcat tgtggaagac gatggcattg gaagaaaggc atctgcaaca aggtcgagga
     5461 tcagtgaaca caatagcaag gcaacacaaa ttatcaatga ttatttacag gcgctaaacc
     5521 aacgcgaaag agcggaagcc tttattctgg atatcgacga tctgacagat gaaacaggta
     5581 aaggcatcgg cacacgcata aggttgagta tgccgctgat catattaaac ccataaacca
     5641 tacagtaatg ttaaaagcaa tcattatcga cgacgaatcg agagcgcgct cagccctaaa
     5701 acaggagatt acacttaatt gccctgatgt acatattatt ggtgaagcgg acagtatctc
     5761 cgcctcagtg tcgctgctgg aaaaggcaga tcctgatatt attttcctgg atatacaatt
     5821 gtcagatggc cttggattta cagtgctcga gcaaatagaa aatgatgact atgccatcat
     5881 atttactaca gcatactcag aatatgcact gaaagcgttt aagaccagtg ccgtagatta
     5941 tttattaaag cccatcgata gtgaggagtt ggttgcagct gtcaacaagg tgaaaaatgc
     6001 gaaggaagag aaacatttac aacaagttgc caattctgcc aggcgcgatt tgaatgcttt
     6061 gcaacaaaag aaaattattc ttcatactac cgaagggctg catgtgatac ctattggaga
     6121 tatcatcaga tgcaatagtt atggcaatta cagcttcgtg ttccttaaag atggcagtaa
     6181 aatattatta gcaaagattc taaaagaaat tgaagaatct ctgcaacatt atggttttga
     6241 gcgtgtgcat cattcacatc ttatcaattt aaaccatgtg aaatcattca gaaacaaaga
     6301 tggaggaatg gtcatgatga gcgacatgag tgaagtccct gtttccatgc gaaagaaaag
     6361 cgcatttctg gatgaactgg agaaactaaa tagtttataa tacaaaaagc cattcagacg
     6421 aatggctttt tttctgcgaa acaaaatggg gctcaggggt attaaatgta actgtgtgct
     6481 tcctagtcag gataacagag tatatatata atgaacaggg aaagccaaaa ttagtgcttt
     6541 cattgagtca gaagagctgt tttggataag tgaaggcaaa tccatgataa ccgttgggaa
     6601 gtaataaaac ggcgcaaaaa ctaccgggaa ttccgtattt acaatgcatc cgtgctctcg
     6661 aatgtttaca aatctttaac aattcttgct tttggcaaca agtgaggata ttcatctgat
     6721 aagtgcaaac aatcgaataa taattgattg ttgatatcgc cattcttaca tggtgcgaaa
     6781 ctgtttattc tatctgaata gataatttta taccacacat tttttctatg tttaatcaat
     6841 ttcaattaaa gggcttgctg ctactattat tggtttgtag cagtctgctt acaaaagctc
     6901 aacacgattg ttcatgtgca tcgtataata aatcgttccg cgatcaatat gaggcattat
     6961 tttctatgcg gaaactggat agtgcacgct tactgagcaa tacgctgata aaacaaaaag
     7021 ggtgctgcga agctgcagga aacatacttg tagccaatac ttttttgata gataaacaat
     7081 ttgacagcat tggtaagttt gttgacagag cagaaaaact aatcggcaac aattatgaca
     7141 cccttgtttc tcctgaaata cttcgtttac gaggggctgt agcagcaggt aaggccgatt
     7201 tggataaagc tgcaagctat tatgcagaag gtattgaaca gtcgaaaaaa catcagaatc
     7261 ttaaatatgc tgctcgattt tgcaatgaat tgagccatac ttatggcagt atgaacaaac
     7321 cggaaccttc attaaagttt ttaaaggaag gtgctgccat cgcagagcaa ataaaagacg
     7381 acaaattatg tgcgatgctt tatgcaaaca tcctcgcaac ttatggtatg tttcatgaga
     7441 acacacataa caaggcatat ctggattcca tggaaatgta tgcaccaatt tccatcaaat
     7501 atgcacaact atccaaagat ccctattaca tcgttcgcgg gtatacatca ttaggtggca
     7561 ttgccatcga aaaaggagat tataaaaccg cgttagcata taatgatacc gttatggcca
     7621 tgttgcctcc tgcgggtgcc gaacaattgc agatatcggc acaatatcga agaggtcagt
     7681 gtttattgca gctgggacgt gttgaagaag ccttggcaag cttacatgca gcgctgggca
     7741 acgctaagaa atttaagagt caaggcatag tgagtcttat caatcaacaa ttgtacttta
     7801 cctatcgaga cctggggaag tcagattcgg cattgtatta ctatgagcag catgtagcag
     7861 ttcgagatag tttggtaaat attgaaaaca cctctgcatt gaccgagtta gaacaaaagt
     7921 atgataaagt tcaaaacgaa aaaaagatca gtgaactcag caaggaaaaa caaatcagca
     7981 ctttgcagat aaggatacta gctgccggca tccttgtgac attgcttgta agtctttcaa
     8041 tattcctgta ttaccgtcaa aaaacattaa acaataaaaa actgattctt gaaacagagc
     8101 aacgactcaa tcgcgcgcgc atgaatccgc atttcttttt taatgcactt gcgtcattac
     8161 agggatttgc attgcgtgaa aatgatggaa aggcattggc gagtaactta tcgaagttca
     8221 gtcatatcat gcgtgaaaca ttggaatcaa cctacaaaga ttatgttacc attcagcagg
     8281 aaatcgattt tctttatgaa tatctcacat tgcagcaaat caggttccct caggcattca
     8341 aattcgtcat gtcggcagat gcattatcgg aacccgacag tttactgatt ccaagtatga
     8401 ttattcaacc gtttgtagaa aattcaatag agcatggatt caggggaatt gattatgttg
     8461 gtgaaataac catattgttt gatgaagacg gggataacat tgttgtttcc attcttgaca
     8521 atgggaaagg ccttgccgga gaaaaggaaa ttacgaatga acatatttcc agagccagtc
     8581 agattatcag agacaggatt tacttgctca atctcaagtt aaaaactaat gcaagtttcc
     8641 aaattgccaa tcaacgcgat ggcaaaggtg tgcaggttgt aatacgatta ccaaaaatta
     8701 ctgaagatga aagtcttaat aattgataat gaaacaccga ttagagtagc attacatgaa
     8761 atgttactag cttattgtcc tgaaatcacg catatcaagg aagcatcaga tatcaaaacc
     8821 ggcattgata ccataggttg gtttaatcct gatatattat ttctggatgt agaattggat
     8881 gaaggcaccg gttttgattt attaagtcaa ctcccggcat ataccttcca attaattttc
     8941 ataacagctc atgataagta tgccatcaat gcattccggt tcagtgcaat ggatttttta
     9001 ctaaaaccga tagatccgga tgacttgatg catagtgtag gaagagcaat caagaatatt
     9061 cagagcgacc cgagtaagca aatggctttt ttattagaac aagtgaaatc aaaacacgag
     9121 cctcccaaaa aaatcatctt aaaggatatg cacaaaactt attatgtgcg tattgaagaa
     9181 attttgtttt tagaggcgga aggcatttat accaagttca accttatggg taatcagcag
     9241 atcatggttt caaaaaatct gaaggaatat gaaagcatgc tcgagccgct tggtttcgtc
     9301 agaacgcatc attcctttct ggtaaatccc gaaaagataa aaatgtttga taaatcggaa
     9361 ggaggtatgc tggtattgga ggatggcaat caaattcctg tttcccaacg caaacgcgat
     9421 ctggtgctta aggccctgga acactaaatt aaagggtaat ttgtatagtt cgacgaaata
     9481 cgctttcgta aaaaggaaat gctgtatctt cacttacaaa gaaatcgtat gtactatact
     9541 tatcaaatca ctattaacaa acctgttgac gaggttgttc gtttattcga taatcctgat
     9601 aatctgtata agtggatgag tgggttacaa tcttttgaac tgataagtgg aactgccggt
     9661 gagcccggtg ccaaatcgaa attggttttt ctaaatggca agcgccgcat ggaaatgata
     9721 gaaactatca cggtgcgcga tcttccggct gaattttcag gcacttatga agctcagggc
     9781 gttgtaaata tcagtgtaaa taagttcaag gatttaggaa atggaactac cttatatact
     9841 accgaaaaca catttcactt ttccggattt atgaaattaa taggcttcat gatgcccggt
     9901 gcttttaaaa agcaatctta caaatacatg gaacaattca aagcatttgt tgaaagtcat
     9961 taatgcaggt atataaaaaa acaaaaaccc ccaatttgga ggtttttgtt ataaatgaac
    10021 agaggatcaa cggatgacag ccaatttttc cgtcatgatc tgtccacttg ttgtatgtac
    10081 aaatatctga tagataccgg caggtatatt gtcgagagcg attacatgtg aaatctcttc
    10141 acctgcacgt ccggcaacta ctgcatctgc gattgggaag cgttcaccat taaggctata
    10201 taattcgata gttgcttcgc catcttcagt catcacaaat acgatttcag cagatgtagt
    10261 agcagggtta gggaaaatat cgataggatt ttcgctgagt gctacttcgt catcaagctt
    10321 catccagcat gctcccaaaa cccaaccgct ggtgagataa cttggcacct ggcctgtagt
    10381 gatacacttg gttgttccca ttttacatac atacactttt gtaagtgaag gtccgcaacg
    10441 aacatctttc acggtcacaa gagctgcatc tgagttgctg catccataat aatctgttgc
    10501 agtgagtgtg taagttgtag ttgtagttgg gcaaacagtt tttgtcaatg aagttccacc
    10561 gccagtccag ctataagtat atggcgatga gccacctgtt gctgtgccgg ttaaagtagc
    10621 acaagttgct ccataaccaa tcgtaattgt tttgtcagta cctgcgttaa cggtaggttt
    10681 tgggtgcaca acaacaacaa ccggatctga atcagtatgt ccatttgcat cagtaacggt
    10741 aacagtataa gtagtgttca ctgttggact tacatttatt gatgctgttg tagcaccggt
    10801 attccacaca aatgaatatg gaggcgttcc acctgttgca gtagcgttta atgttacggt
    10861 atttcctgca cataacgagg ttgtgagacc cgcatcagca gccacatcaa aatttatttc
    10921 catgtcagga gcaccattac aaccataagg ttcaacagca atacctgcag cttcagcacc
    10981 cattaatcgc agttgtgttg gcataccatt ggttactatt gtaaacgttt tgcaatacga
    11041 acctgcggga ccataaccag cgctcgctgt tgtccaactt tgcgctgcaa aaccggttcc
    11101 agtatatgaa tcaagaaaat aaataaggta attagaacca taatagaaag atccgtctgc
    11161 agcatagata gcccctgttt gtggactggc aagtgttgca ggccatgaag aaaaggttgc
    11221 aggtccaacc aattcgactc cccatgtata cgtactttga tcggcaccac cttcacctcc
    11281 acctgcgcag aaggtaacta caaattcata ttggcctcca ccgagatcag tagttgacac
    11341 taaatcgaaa tgagaataat cgcaagcctt tgcataggca aaactgagtg gcagaaggat
    11401 gcctgtcaga aataagtgtt taagtgttcg catatttagc attttaagtt tgggtaaaaa
    11461 gtgcggatga ttccgctttc gaaaccaaaa gtaaatgcct caaaaacccg attttcggga
    11521 aacaaacaaa cgcagggact aaactaacaa gtgatggaat tcagctcagc tccatatagt
    11581 taaggtcttt gccaaatgtc tcttttattg ataaggtggc aatcatagca ataccaatag
    11641 atactgctcc tacaaccatt gcagactgta tattaccgac cgaaacgctc aaacctgcaa
    11701 agctcaaagt gagtggaact accaatccgc gaacgaaatt tggaacggtg gtagtaacgg
    11761 tagctctgat attggtgcca aattgttctg atgcgatggt tacaaataat gcccaatatc
    11821 cggtagcaca tcccaataag aagcacatga tatggaataa tgtgagagaa ctgcttttca
    11881 tattcagata aaccaatgct aatacaacag ttgctatcaa atatcccata acgatctttt
    11941 tgcggctctt aaatacctgg cttaaataac cactcaataa atcacctaca caaaggccga
    12001 tataacttaa catgatagct gttcgcctta cctgaatagc ttcttccggt gtcatttcaa
    12061 tcccccactc ttttgcaaat cgctcgctga atttcattaa tacaccaatg caataccata
    12121 ccggaattcc tatcaaaatg cagtaaacat atttcaaaaa acgctttcta tcgctgaaca
    12181 aagagaggaa ttgacctcgt gctacattct gttgtttcac ctcatcaaac atcgtacttt
    12241 cgaatgtacc gaatcgcagc aacagcaatg ccaaaccaag tccgccgcca atataataag
    12301 atacacgcca atcgacatga gaggtaataa aaaatgcggc tactgctcct aatgcaccaa
    12361 cactcacaat caacatggtt ccataacctc tgtgctcttt actcatgatt tccgcaacaa
    12421 gtgtaatggc cgctcccaat tcacccgcaa ggcctaaccc tgcaaaaaaa cgaacgactt
    12481 gatattgcgt agtattgtcg acaaatccgt ttaaaatatt tgctgccgaa tacaataaaa
    12541 tacttccgaa aagaattgat tttcttccct taagatcgcc cataatgccc cagatgatac
    12601 cgccaaccaa catgcctatc atttgcacat tgaataatcg cacatcccaa taatcaacca
    12661 gggaaggatc agtaatgcct atgcctttga gactggtttt acttaccaga ttaaagagct
    12721 gtaggtcata aatatcaaca aagtaaccca atgctgctac gagcacggta aggttgaaga
    12781 tggtagtacg ccgctggtta ttgttcattg cggcgatgaa attatctaat ttttctcata
    12841 gacgagaaaa tcgtgatcat aaggattttt ttcatctatc gttcttacat cttttgaggc
    12901 cagatgaaac ccttcgccgg gggtggcaaa ataggtatca ccttcaaaac ttgcatgaat
    12961 gcgggttaaa tatatcctat ctacaaacga catcgcctga cgatagatat caccgcctcc
    13021 aaggataaac acttcttcct cctgttgaat caaagggtga tgccttgctt cattcagatc
    13081 atgcactacg atgctgtcat ctgacttgaa attcctgtcg cgggtaacga tgatgaattt
    13141 tcgattagga agcactttat tgccgagggc attatacgtt ttgcgaccaa gaatacacca
    13201 atggccggaa gttttctctt tgaagtattt cagatcggag gtaagcttta accatggcat
    13261 atcgccttgc atgccaatga cctcattttc ggaggcagct acaataatgg aaaataaagg
    13321 catgatactt ttttaaacgc tcacttttgc tttgatatgc ggatgtggat cataattcac
    13381 caattcaaaa tcttcaaatt tgaatccgaa aatatctttc acatcaggat taattttcat
    13441 ttgcggcagt acacgtggct ctctcgataa ttgcaatttt gcctgttcaa tatgatcatt
    13501 atataaatgc gcgtcgccga aagtatgaat gaactcgccg tattgcaaat cacatacctg
    13561 agctaccatc atcgtcaaca aagcataact ggcaatatta aaaggtacac ctaaaaacgt
    13621 atctgccgaa cgctgatata actgacaact caatttacca tcggcaacat aaaattggaa
    13681 catacaatgg caagggggca atgccatatt ttctatatcg gcgacattcc aggcactaac
    13741 aatcaatctt cgtgaatcgg gactcttttt tatttggttg atgaggttac tgatctgatc
    13801 gatatggcgg ccatcagcag taggccaact gcgccactga acaccataaa caggaccgag
    13861 gttaccatgt tcatcagccc attcatccca tatgcttacg ccattttcct tcagataagc
    13921 aatattgcca tcgcctttta aaaaccacag caattcatgg ataatactgc gcaggtgcag
    13981 ttttttggta gttaccagtg gaaacccttc ctgaagatcg aaacgcatct ggtggccgaa
    14041 aatgctcacg gtgccggtac cggtacggtc tgatttcctt gtcccctcat ccagaatacg
    14101 ctgaagtaat gcgtggtatt gcctcatgct gcaaaattac cgatttttag gccgccattt
    14161 aacgattttt gatttgcatc aacatacaat gcacaaacca aaaggcatcg gcattaaggc
    14221 ttggcttaca aattactcta ttattactcc ggcaaatcaa ttccttcaga aataatttca
    14281 tacttcggta agataaaact gagtaccaat gaaattccca tcaccagcaa acaacctatt
    14341 acattatacc aaaggtaacc gatttgaatg gtaggcgtat tccaatgata tctggtgctg
    14401 aagtcaatat acaatatcac caattcgctg ataacggctg caacgaacac cgcatttccg
    14461 cctactcgtt ttaataaaaa cgctgtgaga aaaatgccaa ggatagtgcc ataaaacaga
    14521 ctaccaagga tattaaccgc ctcaataaga ttgtcgaaca tagatacgat gagtgcgaac
    14581 attattgcta aaattccaaa tgctgcagtg agcattttac tggcttgcaa atgttgttta
    14641 cctgtagcat ttttaaacat aaatctttta taaatatcca ccgaaaaagt agtagacagc
    14701 ccatttaaag ccgaagcagt tgaactcata ctggcacaaa aaataactgc aaacaataat
    14761 cctattacac caaccggaag gtgatccatc acaaatcgca tgaaaatata atcatggtca
    14821 tcttgcgtag aaggctccac cttttcaaca attttctgca cttcgagtcg aatactatca
    14881 gctttgcgac cggattcctg atattcatat atcgtttgct ccttcctatt tacagtctga
    14941 cgcgcatttg attgttgcag ttcagaagca agttgatagt cacttgccag tttttcaagc
    15001 tgcggaccat aatccgaatg caatgccttt tgatatgcca catcattaaa ataaacaggt
    15061 ggcgtattga gttgataaaa cgtaaaaacc aataagccga taaataaaat aaaaaactgc
    15121 accggaattt tcacgagccc gttaaacagt aatccaagtc tgctctcgcg taccgatgca
    15181 cctccaatgt atcgccccac ctgactttga tcggtgccga aataacttag ttgaagaaat
    15241 aaaccaccta ttaatcccga aacaatatta tacctgtcgc tccaatcaaa atgacctgat
    15301 tcgctgcctg ttgtaatgac atgcatcttg cccatataac cggcaaggct aattccttca
    15361 cctaaattta tatcacgcgt aatgtaatat accaaaaatc caaaggcagt aaacataccc
    15421 gcataaatca caagcatttg ttgtttatgg gtaacggtaa ctgccttatt tccgccactt
    15481 aaaatataaa gggtaacaat tccaccaata aaaataacgg ttgaggtcaa aggccatccc
    15541 aacaattgcg aaagaatgat agatggcgca taaatggtga tacctgtaga aaatgctcgc
    15601 tgcacaagaa acaacacagc cgttaacgtt ctggatttta catcaaacct gttttcaaga
    15661 aattcataag cagtatatac ttttaatcga taaaatatcg gtataaaaaa tacggatatc
    15721 acaatcatgg ccagtggaag cccgagataa aattgaataa accctaaacc attatcataa
    15781 gcttgtcccg gaacagaaag aaatgtgatg gcactcgctt gtgtagccat tacactaagt
    15841 cctatctccc accatctttt ttcattatcg ccacgcaaat aggtttctgc agtatgaatt
    15901 tgtcgcgtgc gatagatacc atacactgct atcaacaaca gtgtcccgaa catcaccaac
    15961 cagtcgatag tactcatggg aataattgac tgaggctata aaaaataaat atttccacca
    16021 gtagcacaat gactacagtg atatataatg ccttccagaa aaatggtttt tcttcaggtg
    16081 tcatttaggt tgtgattttc cacgcatact catcaggttc acaaataagc gataggctcc
    16141 cggaacacct gcaggaagtt cgcggaaaaa taccagtcct gtatatatga acacaccatt
    16201 gccatagtga gcaaccataa gcgctccgtt ttgctccttc tctccgggat cagttaagcc
    16261 cagtggaaat gcataagatg aatcagcctc tccaacaaaa tatattcctc gctcctgtac
    16321 ccatccttta aaatcatttt cagtaatctt attaggtgta ttcaatgccg gattatcagg
    16381 caataaaaaa ctcaccggag attgttcatc tgtaaccctg ttgcgtgtaa gcttaaatgg
    16441 atatggtccg agttgatcaa cgtatgaatc ccaattagta gaatactgtg ttatcaaagt
    16501 tcctccggct tgtacataat ccattaacaa tgcctgcttt tcagccatcg ctttggaggt
    16561 attatatgct ctcacgccaa taattacaac aggaaattgt tgcaataatg cctgtgtaat
    16621 gtcttcagga tttattgtag tgacctgcac gccaaatgca cgcaaacttt catccacctt
    16681 gtcgccggct ccttcaatat atgcaacggg gaatgttggc acaggcgatt gaatgcgtac
    16741 catgcgcact tcagcatgct ccaaaatctg ttggattgga atatgatcgt agctgattgt
    16801 ttgcaaatcg cgataaacgc cttttttact gcctacaata taattctcaa ctgcatggta
    16861 aacgccggaa tgtatcaagg aatcatatat tgcctttgca tttttgcccg ggataaaata
    16921 caattttaaa ttgcctgttt ctaccttgct gttagttggt gcttgaatag aaaattgtac
    16981 cgcaattgtc tgaccggcct gcaatgtacg aatctcacgt cccccgctta caataaatcc
    17041 ctcgtcatta cttacaaaca caaaaccatc aaggtcttta atgttcgact ccacaattac
    17101 atctacaaac agaggttcat tatatctgaa catcaacagc gatttattgg cacgaatcag
    17161 cgcctttggc agaacagtca atggttgaat aatctcacct ttaacagcat ctgtttcctt
    17221 atagatcact ccgcgcttga aggcaaaaga acggtcacca atcttcacat gatagatcac
    17281 agaaatagca ggatcatttt ctgctttacc aatgaggctt cgatctctga catcaaataa
    17341 ggtattataa tcattctcca gccaataagg cgaggaggtg atgccttcgg gaacttcaaa
    17401 tatgatcgtg tcgatttgat taatattggt aggtaatatt tcaccctctt tgtaagtttt
    17461 tcccaatatt tcaatcgact ctaatgtaat aggataatca agacgcttta cggcagtgct
    17521 tacaatggta agtgattcac cggctgccac tttttctgtg gttgctgcag gctctaaaaa
    17581 taatccaata catgctccga taatttgctg tgcttgctga atccgtctta ttacgatggg
    17641 atcttgcgga aacgtctcaa gagttttaat gtaatctatc aatattggta tcgaacgatc
    17701 aggatgcttg gcatcaaaat tttgaaaggc tgatgaagct aatgctaatg ccgaaacctt
    17761 attttcatat acagaccaat caataggtaa tccggaaaaa atatcattat tcgaaaaagc
    17821 ctcgcctttt ttcaattcga gatattcttt ttgttctccg cgtgtcggtg cactaccgaa
    17881 accttggctc ttgtgattgg tgcgactttc agcagcaatt tcaccatagc tttttcctaa
    17941 gaaggcatta taccccccca catctatcac agcaagtgta ggatctgctt tcgcaatttt
    18001 ttcaggcaat tgcttatccc accaagtaga attattccaa aataatcgct ttgcctgcca
    18061 tactgaaaca tatttcaatt ggtcaggaaa ttttgttgga tcagcagccg catcaaaagc
    18121 ttcttccgca agcatggcag aggctgtgtg gtgaccgtga cctgcgcgtt cgtcggttgg
    18181 gaatctggtg atgatcacat cgggttgaaa attgcgaatc acccaaacta catcggctaa
    18241 taaactatca tgtggccatt tggtaaaagt ttctgatgca cttttgctat atccaaaatc
    18301 tactgcacgc gtaaaaaatt gttcgccacc atccattctt cgagcagcaa gtaattcctg
    18361 ggtgcgaatg accccgagat atgctccctg ttcctcgcct accagatttt gacctccatc
    18421 acctcttgtc aatgataaat aacctgtgcg aactgttttc tcattcgcca accaggtaat
    18481 cagtctggta ttttcatcat ccggatgcgc agcaatataa agcaccgaat gtgatgaagt
    18541 taatttttca atttccagtt gaatttccga tgccgaccag gaaggatttt gagcgtgcag
    18601 attcattgcc tgcatcaaaa acatcagagc aaaaaaatta cacgagatac taatgagctt
    18661 cattaaatat ttattgttat gcctttgaat tttaaacaac acaagttttc agcaaacatt
    18721 aatcaataat atactatatg caaatgtctt gctgaaagaa atgcttgttc aaaaatagca
    18781 atattgccgc agattacagg ctgaatcagg gtaacaagcc ttttgaaata tcaacaggcg
    18841 ctgataatgg caaatgcgat gagcaaccgg tcatgcagtt ttgacttcaa taaaaaaaca
    18901 aggtaaaaaa caaccggaaa ataccaatat cgcaatcagg cagaagcagg aagcttaata
    18961 gccatcatgt aattaatacc gggaatggca tgctcagatt taatggtctg cccttcgagc
    19021 gatgatttaa tataatgggt aagatatgcg taaccaccct ggatttccac aacatgaatg
    19081 cggttcaact catcaatggt aaaggtaatg atcacgtttt gtttatcagg atcgggtaga
    19141 attgtgctta ctgaaacttt cttttcaccc tctttggcgg cgtaatgttg caccgtttca
    19201 tcaataacgg cagttgcttt acgaatgagg tcctctccgt cggcagtagc tgcggaagca
    19261 aagtgtcctg aaagaaccaa cgcgaacagt atggtaagga gttttcgcat attcgtgttt
    19321 ttcgtggtgc ctcttgcacc gtgaaattaa tgagttttaa tgtttctcat gcgtctttaa
    19381 cgcaatttaa cgctatgacg tatgaaccgg ttacaaagtt acaggcatac aatacttttt
    19441 aactaaattt ttcgactagt cggcactttt tgagggcgaa tcgttaaaat tgccctgtca
    19501 tgccccatca ggagcccatt tctattcgtc ttgccttatc cgcagatgcc cctttactcg
    19561 caaaactggg tgctaaaacc ttttttgata cctatcaggc tttcaacact cccgataaca
    19621 tgactgatta tatttctaaa aacttcaata tcaatgcttt agaagctgaa ttgggtgacg
    19681 aagctgtttc attcttcatt gctttttcag gtgattcagg tgttggatac ataaaactaa
    19741 gtcaaggtgt ttcttcgtat gtaagcgaaa atcctgccat ggaggtgtcg cgttactatg
    19801 tagatgccgg tttccagggt cggaaagtgg gtgcagcttt gatgcagtca gcttttgagc
    19861 atgccaaagc caataattgc aaggtgatct ggctggcagt ttggcagaaa aatccaaaag
    19921 ctgtgaacat ttacatgcat cagggttttg agatcattgg caccaccact tttcaacttg
    19981 gcgacgacct gcaggatgat tatgtgatgg ccaaaagatt ataaatgttt ttaagcatca
    20041 tcattccaac tttcaacgaa gcaggattca tcggcaatac cttagaattt ctgctcaaac
    20101 agacctatga catgccattt gaaataattg ttgcagatgg agggtcgacc gatgatacaa
    20161 ctagtattgc atcaaacttt cctgtcagtg tgctgcagag tccggaaaga gggcgagctc
    20221 agcaaatgaa ctttgcagca cgatttgcaa aaggaacgat acttttcttt gtgcatgcag
    20281 attgccttcc ccccgccaat ttcagctcct tcatccgcaa tgccttaaac aaaggggtag
    20341 acgccggttg ttttcgatac cgcttcgaca gcaataagcg catgttacaa ttcaacagct
    20401 ttttcaatcg cttcgggggt ttgctttttc gaggcggtga ccagacgctt ttcatcaaaa
    20461 aggacatatt ttctgcattg ggtggatttg atgaatctta tatcgtgatg gaggattatg
    20521 acatggtcag acgtatcagc aaaggacatc gctttgaagt gctgcgcgat gaggcgatgg
    20581 tttccgcgag gaagtacgat gaaaatagtt ggttaaaagt gaatatcgca aatgccgttg
    20641 ccatgagcat gtatttgacc ggactgtttt gtccgcttac catccgcaat acttatcacc
    20701 gcatgattaa acatcctaag gggcgttaag tatctttgcc gccatgtttg caggcaaggt
    20761 aatatggatc accggcgctt cttcaggaat tggcgaggaa ctggcgtatc aacttgcaac
    20821 gaaaaaagcc tatctcattc tttctgctcg ccgcacactt gaattagagc gcgtgagaga
    20881 acaatgtttg gtgcatacca attgcgttga cattttatca cttgatttat ccgataacaa
    20941 tgctcttcct gaaatggcta aacgcgcaga agccatacac ggtcgcatcg atgtgatcat
    21001 tcatagcgga ggcatcagtc agcgtgatag catgctgaac actgttcccg atgctgaagc
    21061 gcgtatcatg caaattaatt tcacatcagt agttactatt acgagagcgc tattgccggg
    21121 aatgattgca cgaggtgaag gacgtatcgt gttgctgagt agtgttgccg gaaaaattgg
    21181 tgtgccgaac agaactacct attgcgcaag taaacatgcc attattggtt atttcgatgc
    21241 cttgcgggct gaattaaaaa tcgctaagca acccataaat atacatatta tggtaccggg
    21301 atttattcat accaatatca gcgtaaatgc ggtgttgggt gatggcaatc ctcaaggcgt
    21361 gatggatccc ggacaagaaa aaggcatgcc ggtagaaaaa tgtgtgcagc agatgatacg
    21421 tggcatggag aaaaataaaa aggaaatatt aatcggaggg aaagaaattc taatgactta
    21481 tttccgccga tttattcccg gtttatatta ttatctcatt accagagtta aaacaaaata
    21541 atcgatcaga attcagttgt tatgtcagat aaacccatta tttcaggaat tcaacaagta
    21601 ggtattggca atccatatgt gcatgaagca tggcgatggt atcgcgagaa cttcggtatg
    21661 gatgtgaaaa ttttcgaaga agctgcagaa gcgaatttaa tgttgccata caccggtgga
    21721 atgcctcata aacgtcacgc tgtattggcc attaatatga acggtggcgg aggttttgag
    21781 atatggcaat acacatcacg aaaacccgta gcaccggcat ttcaagtgca gtatggcgat
    21841 ctgggaattt atgcacgtaa aatgaaaagt cgcgatgtgc aggctactta caatttcctg
    21901 aaaaatcgcg gaattaattt gttgggcgat gtcaccaat
//
LOCUS       DAOQOU010000039        21380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1189_length_21380_cov_6.408206, whole genome shotgun sequence.
ACCESSION   DAOQOU010000039 DAOQOU010000000
VERSION     DAOQOU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21380
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_21380_cov_6.408206"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLM90_05305"
     CDS             <1..1044
                     /locus_tag="PLM90_05305"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HMZ88819.1"
                     /translation="LGGSLMDEKKAVHPNDDVNKSQSSNDTFPTAMHIAAYKILLECT
                     IPGIEKLRNTLAEKSLAFMHVVKIGRTHFMDATPLTLGQEMSGYVSQLDHGLRAIKNS
                     LAHLSELALGGTAVGTGINTPEGYDVLVAKNIAALTGLPFVTAENKFEALAAHDAIVE
                     SHGALKTVAVSLMKIANDIRMLSSGPRSGIGEIHIPDNEPGSSIMPGKVNPTQCEALT
                     MVAAQVMGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGDACVSFNDKCAMGIE
                     PIEANIKKHVDNSLMLVTALNTKIGYYKAAEIAQKAHKEHKTLKETAVELGYLTPEQF
                     DEWVVPVQMVGKL"
     gene            complement(1211..1987)
                     /locus_tag="PLM90_05310"
     CDS             complement(1211..1987)
                     /locus_tag="PLM90_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HMZ88820.1"
                     /translation="MRKNIISMIVMLPILVVLVATSSCKDDTADRQAHVNIYLTDAPA
                     EYDAVYIDIQAVEITSDMGVETFDVVTPGVYNLLEFNSGIDTLIISEDVSPRTISQIR
                     LILGSNNTVVVDGESHPLETPSAQTSGLKLNVHYTFEAGLVYDLWLDFDAEQSIVEKG
                     NGDYSLKPVIRVYTEALSGAITGVVFPTDAAYYVQAMSATDTAGTYISADGSFLIGGL
                     PEGSYSVNFSAVAGFTDITLPGVSVTTGIITDLGEVTIPL"
     gene            2358..3050
                     /locus_tag="PLM90_05315"
     CDS             2358..3050
                     /locus_tag="PLM90_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88821.1"
                     /translation="MHRVIFVGIGLCKKIIWKSILIYYLSIKTHAKQENKLLVAAVAL
                     TMVFSANRASAQAFYQGVNHLSLGVGFGGYLDYAYTGSGDFSHIPTLFASFDHGTIDD
                     VFGGNIGIGGFVGYSSAKYNYDYLGYHDDSKWTYFAIGARGTYHYILDNENLDLYGGI
                     SIGVLSQSYDYNSNYPWVGFDPNSYYDYNNMVLYSSACVGAKYMFSESLGAFAELGWD
                     IAWLKAGVTLGL"
     gene            complement(3117..3311)
                     /locus_tag="PLM90_05320"
     CDS             complement(3117..3311)
                     /locus_tag="PLM90_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88822.1"
                     /translation="MSDVAWIAVIIIIYIAFIKPMLQGARQSSQQQGGNNIREKKNPA
                     GTSEKTNSKDADYVDYEEIK"
     gene            complement(3383..3757)
                     /locus_tag="PLM90_05325"
     CDS             complement(3383..3757)
                     /locus_tag="PLM90_05325"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ88823.1"
                     /translation="MFLDLSHKKLPIYLASLTFVRECYKVLDTVNSSERFGIEKQIKR
                     AAVSVHLNIAEGASKRSKIDRRRYYEMARGSLVEVDSGFDILVTLNLIQNTELHTLNN
                     AVNTCFQQLSNLINYLSSEIKS"
     gene            complement(3947..6778)
                     /gene="carB"
                     /locus_tag="PLM90_05330"
     CDS             complement(3947..6778)
                     /gene="carB"
                     /locus_tag="PLM90_05330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMZ88824.1"
                     /translation="MPKDTSIKHVLIIGSGPIIIGQACEFDYSGSQASRSLREEGIKV
                     SLINSNPATIMTDPVTADQIYLWPLNVGSIEKILIENIEKGTPIDAVLPTMGGQTALN
                     LAKEAFELGIWVKYSVRMIGVDIEAIDRAENRELFRQLMVELGIGVAKSHVANSFLEG
                     KEFAQEIGFPLVIRPSFTLGGTGGGFVKVKEELDAALMRGLTASPTHEVLVEQAVLGW
                     KEYELELLRDANDNVVIICVVENLDPMGVHTGDSITVAPAMTLSDTCYQHMRNLAIKM
                     MRAMGNFAGGCNVQFAQDPYTENLIAIEINPRVSRSSALASKATGYPIAKIAAKLAIG
                     YNLDELNNQITKSTSAYFEPALDYVVVKMPRWNFDKFPGADDTLGLQMKSVGEVMSIG
                     RSFQEALQKACQSLENNKIGLSGEKGFYKSSQEIIDSLAKPHWDRIFNIKKAMDLGVP
                     VRTIHNQSLIDPWFLMQIQDLVKLEKELRRYQLTNIPKKLFLELKQKGYSDIQIANTV
                     GNCTEEEVYNHRRSLGIKRTYKLVDTCAAEFEAKTPYYYSTFEDENESIVSDRKKVIV
                     LGSGPNRIGQGIEFDYCCVHGVLAIRESGYEAIMVNCNPETVSTDFDIADKLYFEPVF
                     WEHLWELIEYEKPEGVIVQLGGQTALKLAKQLHEKGIKIIGTSYNDMDIAEDRGRFSD
                     LLKDLGIPYPEYGAATTVEEALEVAHRIGYPVLVRPSYVLGGQRMRIVINDEELERSV
                     IGILKHFPDNRILIDHFLDRAEEAEIDAICDGENVHIMGIMEHIEPAGIHSGDSSAVL
                     PSFNLSERVLEQMKAYTEKIAFALNIRGLINIQFAIKNEQVYVIEANPRASRTTPFIA
                     KAYQIPYLNVATKVMLGVKKLSEFTFDKKLQGYAIKEPVFSFEKFPNVNKELGPEMKS
                     TGEAIRFIADLQDPFFREMYSKKSMFLSR"
     gene            7009..7821
                     /locus_tag="PLM90_05335"
     CDS             7009..7821
                     /locus_tag="PLM90_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ88825.1"
                     /translation="MTAAGDFQFGDAVFHYTRFGNGTEVMLAFHGFSEPGTSFLPLEP
                     ALGLRYTIYAFDLPWHGETQWPEGRIFTPADLLGLVRGFLDYLGASKFAVLGFSMGGK
                     CALYITKHMPDRLNALWLLASDGIQTNRIYNVAVYPAWGRKLFKTTIRHPSWFFGMVN
                     LATRLRLITPWLKKFTNNHMATREKRQRLYDTWISMAGFNPDIELVKKQLNTFHIPAL
                     LFFGRRDEVIPYTVGEQFAEGVALAKLTVLERGHYFIDEKINPMLEEAMHFI"
     gene            7863..8987
                     /locus_tag="PLM90_05340"
     CDS             7863..8987
                     /locus_tag="PLM90_05340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88826.1"
                     /translation="MLFITIGTILLIAGYVSLVAFFLYGWWKLKEFNGTATKKHVFIT
                     VIVPVRNEEKNIVHLLRDLKEQTYPADKYEVIIVDDHSTDQTTQKVMQAKMDNVRIIS
                     LGDAGTVRMNKKAGIQHAVSSAKGELIITTDADCHVGNKWISTIAAYYTEHEPVMIAG
                     LVNYFPDATFFGKFQTLDFLSLVGIGAACIQNGFYNLCNGANLAYTKAAFEAVNGYEG
                     VDHIPSGDDMMLMHKMARKFPGKIASLKNKDSIVYTHTETDVVSFWHQRVRWTSKSTH
                     YEDKRITLILSFAYLFNLLIVFNMVIGFFVPGMLKLSMWMFLVKICIDTLFTYAVSRF
                     FRRENILWLFLPIQALHVLYIIIIAPAGVFGKYQWKGRRH"
     gene            8992..10383
                     /locus_tag="PLM90_05345"
     CDS             8992..10383
                     /locus_tag="PLM90_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88827.1"
                     /translation="MKFKRKLLSILIFVAASGAIAVLWKLLDTDIDWFVAIYNSITAV
                     MVIYVGTLMLFIYPIAFLRKYWEKKKSQIDLNAKPKYKVYDRLMRNTNALAGGLFILW
                     VALAAIFAHIIIPDKSPYAGDQMNEIAYKEPGFSVKVLLVPADRHVSSTGVIDWFLNG
                     KTSSFKQIPINDAWLVKGDSLYVSRYLGDNMVGIPMVFATEFITGKKSVVAEQSQIIE
                     NEFIETRHFWLGTDDLGRDYLSRIILGARVSLSVGIIAVLIALFIGIPLGALAGFYKQ
                     YPPKLRFGKKRAVKVPVDNSVMWMINIVWSIPTLLLVFPIVFAFGQQYYTIFIAVGIT
                     MWVDIARMVRGQVMQVREMEFVQAARTFGFSDTRTIWRHVLPNITGPVIVVTAANFAY
                     AILTEAGLSFLGIGVQPPAPSWGLMIAKYKDNLITDPYLPLIPGFAITLLVLAFFMVG
                     NGLRDALDIKSRL"
     gene            10522..11772
                     /locus_tag="PLM90_05350"
     CDS             10522..11772
                     /locus_tag="PLM90_05350"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ88828.1"
                     /translation="MAIAGLNVMEMLRNQLKLKQLEIDSLLEITKSINANKSASALFE
                     LYHRTLRDQIGVENLLLFSNMGGDWEAMIGDKLISEDHPEVMQYLLPYEKLTDLTTVD
                     IPDYLSEYDLLIPVLHKDKPLAYLLMSNPRVESYEPLEDKIKFAQTVTNIVIVAIENK
                     RLFKREVEQQAFKRELELAAQVQSMLIPDTLPHTEQVQMAAIYLPHKNVGGDYYDCLE
                     LNNKEIFFCIADISGKGIAAAILMANFQAQIRELTKRNPPTIEEYMQELNKGVLMSTK
                     GEKFITLFLAKYDRTTGYLKYVNSGHNPPILVNSQGTKLLDKGTTILGMFDTLPSVKM
                     GQLPITEDTAIVCYTDGLTDVVNEFNQVFPLDELIELAKQHYKEPVEVLNKSIIDEIM
                     HFKGHDGEVSDDITVLSVKIHPAN"
     gene            complement(11769..13232)
                     /locus_tag="PLM90_05355"
     CDS             complement(11769..13232)
                     /locus_tag="PLM90_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ88829.1"
                     /translation="MLKTLEKPVEFSIYWIATLLAIVAIFAGVILEEPLYFAVPVAFL
                     FAYQMIVDFRVIFFLLLIATPFSIEYYSSSGFSTTLPTEPMMIIFMFTFIFFIFLKRE
                     LFDTGFFLHPLTIILYVHFAWMLVTTMFSEDIVISLKYMLAKTWYITTFYFMTSLVIK
                     DIATFKKAFWCLFVPTLALTIYTLINHNKYHFAFSEVNKTMVPFFRNHVNYAVFLALM
                     LPMLFSAYRWYPKNTWQRWIIQFGIVVLIAGIYFSYTRSSWLSVAGATIAFFIIRMNK
                     LVTSAVFAVFGVIIFAIVMLHNNRYLDYAPDYKKTIYHTNFNEHMESTMSLEDVSSAE
                     RIYRWVAAIHMIEDKPYMGFGPGQFYFNYKEYTVNKFETYISKNPERSTVHNYYLQVT
                     TEQGFPGTFVWVVLLIYTMYLGQRLYHRFTNIEYKHISMSITLSIITIIINLALSDLV
                     EADKIGTSFFMFLSLLVSFDLLLRRNPALSAEAPAKD"
     gene            complement(13225..14124)
                     /locus_tag="PLM90_05360"
     CDS             complement(13225..14124)
                     /locus_tag="PLM90_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HMZ88830.1"
                     /translation="MEQRNDLIRLLQIIFKWRKPIIITTVVVAIAACIITWFFMPDYF
                     KSTVNFYPSNPIMTDRQVLFSQSTGEIEIDYFGTASDVDRILTIANTSSLIDYVINHF
                     HLMEHYDIDSTKEMARYNCKKEFLKNYSAVETEYGAIEVSVWDKNKDTAMHIANHIVE
                     TIDQHNKDIIMRDKKLIVNTFKNQVAKKELIVTNLTDSIAAQRKAGASAEMMIVLDSK
                     LQSAVDDLNLDRKLLEQNETTLNTDFSTVHITEAAYPALRKDKPVRSLIVIGVTLGTI
                     VFMILLAVITENYRKIKSRIQNA"
     gene            complement(14161..15630)
                     /locus_tag="PLM90_05365"
     CDS             complement(14161..15630)
                     /locus_tag="PLM90_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMZ88831.1"
                     /translation="MRKNFITNIGFLLFLNLLVKPFWILGIDRTVQNTLGPEVYGVYF
                     ALFNFSYLFQVVLDFGINNYNNRQVAQDPQTLGRNIMSTLVLKLLLGVVYTIIVFIAA
                     FGVQFSGEQFQILGLLVLMQIALSFYTFLRSNVNALHLFRTDAVLSILDKLTTSILCA
                     WIFWGASGITMSIPLFIWIQIGGFVVAILVAVAVLSRQKLTISNRIDKALIKNIFKGS
                     LPYALLGFLMTAYYRTDGVMLERMLGDKGPYQAGIYASAFRLLDAMSILGFLMAGMLM
                     PMFARMISQNTSVKPLLDLGFQIMLICSISAGIACLIHREDIMQLLYHVHDPSAGSIF
                     GWLMASFICISLTYVYGSLITAHGSIKMLNYISFGGFILNIILNLMLIPEQQALGSAK
                     ATVFAQVLILLAHIAVAHRLFSLGSNLSKWLRSIFYLAAVIGVNLLIAVFNISWTWQW
                     VIGLASGAFLAIPAGIINMTEVRSLKTVMQSAIARRQGS"
     gene            15766..16929
                     /locus_tag="PLM90_05370"
     CDS             15766..16929
                     /locus_tag="PLM90_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ88832.1"
                     /translation="MLIAVNTRFLIKDKLEGIGWFTYETMKRLTRNHPEHHFLFLFDR
                     DWDPEFIFSDNITPVKVWPPARHPYLWKYWFDTALPRIFKKYKPDLFISTDGMMSLAV
                     NVPTVLVVHDLAYIEFPHHIPRLTYEYLKHFTPKYLSKADAIITVSDYSKKDIIDKYQ
                     IADSKIDVVANGASDNFKPLDEVNRSIVKQKISSGSEYFIYVGSIHPRKNVGMLMKAY
                     DQLRAEGICDHKLVIVGRYAWKSGETYNIYKSMQFIDDVIFTGSMNQEQLTEAIGAAD
                     ALVYPSLFEGFGIPVLEARYAGVPVICSNSSSLPEVGGEHAIYFDPNSAEAITTAMVQ
                     FLKNRAELKRNALESAYEARNIYNWDNSANKIYSIMQATYDNYRANRKYTANT"
     gene            16886..17575
                     /locus_tag="PLM90_05375"
     CDS             16886..17575
                     /locus_tag="PLM90_05375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ88833.1"
                     /translation="MTTIEQIENILQIPDTDKKTPVRVEEAKFIYQFILGHKIHKTLE
                     IGLGYGRSASYIMLATGKPHIAIDPFQSHYGYQAIKNITDAGLKDNFTHIEDFSHFAL
                     PELCHKNEKFDFVFIDGDHKFDGILIDYYFASMLVKTGGYILFHDTWMRSTRLVNKFV
                     EQNRHDFKTHPVSLANLHLIQKIQDNDTRDGMYFREFYTTKSWLKYRLITHLNDHPKG
                     LLSKLKSIFRP"
     gene            17586..19259
                     /locus_tag="PLM90_05380"
     CDS             17586..19259
                     /locus_tag="PLM90_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88834.1"
                     /translation="MKKLFWLTALCMLLTQVSTAQIYDIYSVIPTFNTVETDNYCSDS
                     PIAVEVVMTAGAITDDDGFSIRMKSYPGGLSWAGYDGLCPDDYNCAYGFSIDPGDVPD
                     GLYYFEASCKFFAHAGDPDYEQSEVFSLKTITCGNVEDPLNWTSAKVRDNTGMSMADG
                     KIYYVDDNRKVRNFYWTGTNWISDVLNTTAPKAKDGVDIVATVENVYYVGDDNKIKNL
                     YWTGTDWAYTSLVASQPEVRSNSNIAFGDGKIYFVSADNNKVYNLYKVGATWYCSALN
                     NDAPDVRDDSKLATDVGKVYFVSSTPLNASTNKMIYNYYWDGDSWEYAMLNSSAPKAM
                     DGAGPAVCNAKVYYTAEDQKVKRLKWNTATGVWDVNSLLATQKLVDAGEQIAVVDDII
                     FFHNSFDLVYYLKVSGHAWNEYSMSPCNAYSSTSYSDIRDNTTIIPEGTGKVYYVGTD
                     RRLRNFYTVACRSSIEISPVPTEILTAFPNPSNGIIKLMNASTEPLSINTLRVVDLQG
                     KMVYVDTESKLVNANASLDLDLSQIPNGVYFILSDAGDEIISNRIIISH"
     gene            19400..21298
                     /locus_tag="PLM90_05385"
     CDS             19400..21298
                     /locus_tag="PLM90_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88835.1"
                     /translation="MVIRMLFSKIGLYVSALGACHCSIDMAYSQKVDNDHRDQYAPSA
                     VVIMHEIKQDYNPIWYSTEMPAPGSNSYRRHLIDLKDELYRNYMPQQQSNLLREETDI
                     TSPEILSGWEGNLITSYVPNDNDLAISNGGMIVSVINSNIYIYDDAGTGLFVTSLQSF
                     ADTLGILDSKFDPKVMYDPRADKFVLLFLAGFDPEHTHVILAFSQTNDPLGAWNLYSL
                     PGNPKDNNRWTDFPMFALTENELFLTINLIIPDEPWQTGFSETLIWQMSLDKGYAGDT
                     LDAVYYDSIFFEGKPLRNLNPVKGGANTYGPDMFFLSNRNFDPANDTIFIVHVTGTLD
                     DPETELTVDFGRSNIAYGVPPQARQFSTHIFDTNDGRVLGSFYENNQIQFVSNTLDPA
                     TGFCGVYHGVITDPTGAKTITANIIGDDTLDLGYPNISYSGKFTGDDQSIITCEHTAP
                     TVYAGVSGIFFNWGGYSDLINIHTGDTYVNILSGAYERWGDYTGSQRKYDEPGVVWMS
                     GTFGKYIDVFPFVQRNNATWIASLRSTVEPAVNVPVNSNMSPARLFPNPTADVFTTEI
                     NLSIAGIIECYLYDMQGRLVKSLLRAEGKAGNNHFQFSMAPLSTGTYQLYITLNSTAL
                     TTLPIIKN"
BASE COUNT         6300 a         4271 c         4471 g         6338 t
ORIGIN      
        1 cttggaggaa gcctgatgga tgaaaaaaag gctgttcatc caaatgatga tgtaaataaa
       61 tcgcagtcgt cgaacgacac tttccccaca gccatgcaca tagcggcata taagattttg
      121 ctggaatgca cgattccggg aattgaaaaa ttgcgcaata cacttgcaga gaaatcatta
      181 gcattcatgc acgtagtaaa aattggaaga acgcatttta tggatgccac accacttacc
      241 ctcggtcagg aaatgagtgg ctacgtatca cagctggatc atggattgcg tgcaattaaa
      301 aattcacttg cgcacttaag tgagttggca ttaggtggta ctgctgtagg aacaggaatc
      361 aatacaccgg aaggatatga tgtattggta gcaaaaaata ttgcagcgct tacgggattg
      421 ccttttgtta cggctgaaaa taaatttgaa gcactcgcgg cacacgatgc tattgttgaa
      481 tcgcatggcg ccttgaaaac agtagcagta tcattgatga aaatcgcgaa tgatatcaga
      541 atgttatcca gtggtccgcg ctcaggaatt ggtgaaattc atattcctga caatgagccg
      601 ggttcatcta tcatgccggg taaggtaaat ccaacgcaat gtgaagcact gactatggtt
      661 gcggcgcaag taatgggcaa cgatgtggct attaatattg gcggcgcgac cggacatttt
      721 gaattgaatg tgtttaaacc ggtaatgatc tataatttcc tccacagcgc gcgtttgatt
      781 ggtgatgcct gtgtatcgtt caatgataaa tgtgcgatgg gtattgagcc aatagaagcg
      841 aacattaaaa agcacgtaga taattcactg atgttggtaa cggcattgaa tactaaaatc
      901 ggttattata aggctgctga aattgcgcaa aaagcacata aagaacataa aacactgaag
      961 gaaaccgctg ttgagcttgg atatttaaca cctgagcaat ttgatgaatg ggtggtgccg
     1021 gtgcaaatgg tgggcaaatt ataaatagct gattcacgaa aaaataaaac ctgcattcgt
     1081 aaaacatgca ggttttattt tttgtgccga tttcaatttt tgtatatgtt gaaatcgact
     1141 tacaactgcg gtattttcct ttacaccgtc gattgctatt tgggaaatac ctttcagttg
     1201 ttgtatatgt ttacaaagga atagttactt cacccagatc tgtgataata cccgttgtaa
     1261 cactgacacc gggtaaagta atatctgtaa atccggctac tgctgaaaaa ttaacagaat
     1321 aacttccttc cggcaaaccc ccaattaaaa atgaaccatc tgccgagata taagtgccgg
     1381 cggtatctgt tgccgacatt gcctgaacat aatatgcggc atccgtagga aaaacaactc
     1441 cggtaatagc acctgatagc gcctcagtat aaactcttat tacaggtttt aatgaatagt
     1501 cgccatttcc tttttctaca attgactgct cagcatcaaa gtccaaccaa agatcataga
     1561 caagacccgc ttcgaatgta tagtgaacat tcagtttcaa tccggatgtt tgtgcagaag
     1621 gtgtttccaa aggatgagat tctccatcaa caactaccgt attgttagaa cctaatatca
     1681 atcgaatttg tgagattgtt ctaggggata catcttcaga aatgatcaat gtgtcgatgc
     1741 ctgaattaaa ttccagtaaa ttatataccc ccggagttac cacatcaaat gtttctactc
     1801 ccatatctga ggtgatctct acagcttgaa tgtcgatgta tactgcatca tattcggcag
     1861 gtgcatcggt taaataaatg tttacatgtg cctgtctgtc agctgtgtca tctttacaac
     1921 tgctcgttgc taccaaaact accaggattg gcaacatcac aatcatactt atgatattct
     1981 ttctcatagt gattcagttt gatgtaatcc atacaaatca gatgccatcg ctataattta
     2041 ttgaaaatca atgagtttat tgcgtatgga aattgattta ttgaggaatt atttccccat
     2101 tgcatgttgt tttgacatca attttgcatg tatcaatttt tggagatcta caatgatgta
     2161 attaactttt attctttcag aaattgtgaa agtcatttct ttacacaaga aaattactga
     2221 ccaattgaaa acattacata tttcactcat tggatgatgt aaagcctggc aaaatattat
     2281 tgcaagcatg agatcacatc cgtctaccgg cactttcggc gatattgcct gtttaccgtg
     2341 aatgtgtact gtttagtatg cacagggtga tttttgtcgg aattggattg tgtaaaaaga
     2401 tcatttggaa atcaatccta atctattacc ttagcatcaa aactcacgca aaacaagaaa
     2461 acaaactact cgttgcggca gtcgccctta caatggtgtt ttccgcaaat cgtgcttctg
     2521 cacaagcttt ttatcaaggc gttaatcatc tgagccttgg tgttggattt ggtggttatc
     2581 tcgactatgc ctacaccgga agcggtgatt tcagccatat cccaacgctg tttgcatctt
     2641 tcgaccatgg aacaatcgat gatgtcttcg gaggcaatat cggaattggc ggttttgtgg
     2701 gatactcttc tgcaaaatac aattatgatt accttggtta tcatgatgat tctaagtgga
     2761 cgtatttcgc aatcggtgct cgcggtactt accattacat cttagataac gaaaaccttg
     2821 atctttatgg gggtatcagc attggtgtgt tgtcgcagag ctatgactac aattcaaatt
     2881 atccatgggt aggatttgat ccaaactcat actacgatta taataatatg gtattgtatt
     2941 catccgcatg tgtaggtgca aaatatatgt tctctgaaag tctcggcgcc tttgctgaac
     3001 ttggttggga cattgcctgg ttgaaagcag gtgttacact tggactttaa tacattccaa
     3061 caataaaaaa accggttgtg caaacagccg gtttttttat tttatattag ttgcaattat
     3121 tttatttctt cataatcaac ataatccgca tccttcgaat ttgtcttttc cgaagtcccg
     3181 gcaggattct ttttctcccg tatgttattc cccccttgct gttgtgacga ttgacgggct
     3241 ccctgcaaca ttggcttgat gaaggcgatg taaatgatga tgataactgc tatccaggcg
     3301 acgtccgaca tgatgaaata ggagagagga aaaaggagaa aggagaaagg ttgattaaag
     3361 tgagattaaa atttgagcaa aattatgact ttatttctga gctaaggtaa tttataaggt
     3421 tagaaagttg ttgaaagcaa gtgttgacag cattatttaa ggtgtggagt tctgtgtttt
     3481 gtatgaggtt caaggttacc aagatatcga aaccagagtc cacctcaacc aaagatcctc
     3541 gagccatttc ataatatcgt cttctgtcaa ttttcgaacg tttagatgca ccttcagcaa
     3601 tatttaaatg gacggaaaca gcagcacgct tgatctgctt ttcgatacca aaccgttctg
     3661 aagaatttac agtatctaac accttatagc attcccttac aaaggttaga ctggccaaat
     3721 atatgggcag ttttttgtgc gagagatcca aaaacataca tggtatttta aaggtaaaca
     3781 atcccctaaa attaaccagt aataacaaat caagtttaat cgcttatcat ccatttaagg
     3841 ctaagtaaca gccaaataga accctatttg cgctttccac ctcaccctcc tctctcctct
     3901 ctcctctctc ctctttcctc tttcctcttt cctctttcct ctctcctcac cttgaaagaa
     3961 acatcgactt cttcgaatac atttccctaa agaacggatc ctgcaaatcg gcgatgaacc
     4021 ggatggcttc gccggtggat ttcatttcag ggccgagctc tttgttgacg ttagggaatt
     4081 tttcgaaact gaaaacaggt tccttgatgg catagccctg taattttttg tcgaaggtga
     4141 attcgctcag ttttttaact cctagcatca ccttggtagc cacattcaaa tatggaatct
     4201 gataggcttt ggctatgaat ggtgttgttc ttgatgcacg tggattcgct tcgataacat
     4261 aaacttgctc atttttaatt gcgaactgaa tatttatcaa cccgcgtata ttgagtgcga
     4321 atgcaatctt ttccgtgtat gctttcattt gttccagcac acgttcactt aaattgaaac
     4381 ttggtaatac cgcactcgag tcgccactgt gaatacctgc cggttcgata tgctccatga
     4441 tgcccatgat gtgcacgttt tcgccatcgc aaatcgcatc gatttctgct tcttcagcgc
     4501 gatcaaggaa gtggtcgatc aatattctgt tatccgggaa atgtttcaaa ataccgataa
     4561 ccgaacgctc aagttcctca tcgttgatta caatccgcat gcgctgtccg cctaacacat
     4621 aactcggacg caccaacacc ggatatccga tgcgatgtgc cacttccaat gcttcttcaa
     4681 cagttgttgc tgcaccatat tcagggtatg gaatgcctaa atctttcagc agatcagaga
     4741 acctgccgcg atcttccgca atatccatat cgttatagga tgttccgata attttgatgc
     4801 ctttttcatg caattgcttg gcaagtttta atgctgtttg tccgcctaac tgcacaataa
     4861 caccttcagg tttttcatat tcaatcaatt cccataagtg ctcccagaat accggttcga
     4921 aatataattt atcggcaata tcaaaatcag tagaaacagt ttcaggatta caattcacca
     4981 tgatagcctc atagccgctc tcgcgaatgg ccaatacacc gtgcacacag caataatcga
     5041 attcaattcc ctggcctatc ctgtttggac ctgaaccaag tacgattact ttttttcgat
     5101 cacttacaat actttcattt tcatcttcga aagtagaata ataatatggc gtttttgcct
     5161 cgaattcagc tgcgcaggta tcaaccagtt tataggtgcg tttgataccc aatgatctgc
     5221 ggtgattgta aacttcctct tcggtgcaat tgccaacagt atttgcaatt tgaatatcgc
     5281 tgtatccttt ttgttttaat tcaaggaaaa gttttttagg aatattagtg agttggtatc
     5341 tgcgcaattc tttttcaagt tttaccagat cttgtatctg catcaggaac caaggatcta
     5401 tcaaactttg gttgtgaatg gtgcgcacag gcacgccaag gtccatcgct tttttaatgt
     5461 tgaatattct gtcccagtgc ggttttgcca gactgtcgat gatctcttgt gaagatttgt
     5521 aaaatccctt ttcaccactc aatccgattt tattgttttc gagactttga caggctttct
     5581 gcagtgcttc ctgaaaactg cggcctatac tcatcacttc accaacactt ttcatctgca
     5641 atcctagggt gtcatctgca ccgggaaatt tatcaaaatt ccatctcggc atttttacta
     5701 ccacataatc caatgcaggt tcgaaatatg ctgatgtaga ttttgtgatt tgattattga
     5761 gttcatcgag attataacct atcgccaact tcgcagcaat ttttgcaatc ggataaccgg
     5821 tagcctttga tgctaaagcc gatgaacgcg atacacgtgg atttatttct atcgctatca
     5881 ggttttccgt atatggatct tgagcgaact gcacattaca tcctcccgca aaatttccca
     5941 tcgcacgcat catctttatg gcgaggttgc gcatatgttg atagcaagta tcactcaatg
     6001 tcattgccgg tgctaccgtg atcgaatcgc cggtatgtac tcccatggga tccaggtttt
     6061 ctacaacgca tataattact acattatcat tagcatcgcg cagcaattca agttcatatt
     6121 ctttccagcc caataccgct tgttctacta atacctcatg cgttggcgag gctgttaatc
     6181 cgcgcatcaa agcagcatca agttcttctt ttaccttcac aaagccgcca cctgttccgc
     6241 caagcgtaaa tgatggtctg attacgagcg ggaaaccaat ttcctgggca aattcttttc
     6301 cctcaagaaa tgaattcgca acatgacttt ttgcaacacc tatgcccaat tccaccatca
     6361 actggcgaaa taattccctg ttctcagcgc ggtcgatggc ttcgatatct acacctatca
     6421 tccgcacaga atatttcacc catattccca actcaaaagc ttcctttgca aggttgagcg
     6481 ccgtttgtcc gcccatggtt ggtaacaccg catctatcgg tgtgcctttt tcgatatttt
     6541 ctatcaatat tttctcgata gatcccacat tcaacggcca caaatagatt tgatcggcag
     6601 taaccgggtc ggtcatgatg gtggcgggat tggagtttat taatgaaact tttatccctt
     6661 cttcgcgcaa actgcgactc gcttgtgatc ctgaataatc aaattcacaa gcctgaccaa
     6721 tgatgatggg tcctgatcca atgatcaata catgcttaat agaggtatcc ttcggcataa
     6781 tgtccgttgt atgataataa ggttaggaaa ataaaatggc cttggggagt aaaagagagc
     6841 tgatgaccgg cctgaaaatg catttcagga tgcaaattta cggtacttcg accgcttttc
     6901 ctgctacttt ttcagttatc aacaatccat gcatgaaaat gccggttatt caccgctggt
     6961 agagcaaaaa tggtggatta aagcttcggc tcgtcgtatt ttcgccttat gacagcagct
     7021 ggggactttc aatttggcga tgcagtgttt cattatacga ggtttggcaa tggaactgag
     7081 gtcatgttgg cttttcatgg cttcagcgag cccggaacga gctttttgcc cctggagcct
     7141 gccctaggcc tccgctatac gatttatgcc ttcgatcttc cctggcacgg agaaacgcaa
     7201 tggcctgaag ggcggatttt cacgcctgca gacctcctgg gactcgtaag gggctttctg
     7261 gactacttag gcgcctcaaa atttgccgtt ttagggttca gtatgggcgg gaaatgcgct
     7321 ttgtatatca ccaaacatat gcctgacagg ctcaatgccc tgtggttgct ggccagcgat
     7381 gggattcaaa cgaacagaat ctacaatgtg gcggtttatc cggcatgggg acgaaaactg
     7441 tttaaaacaa cgattaggca tccatcatgg ttctttggaa tggtgaattt ggcaacgagg
     7501 ctgagattga tcaccccctg gctgaagaaa tttaccaata accatatggc tacgcgtgaa
     7561 aaacgtcaac ggctttatga tacctggatc agcatggcag gatttaaccc ggatatcgaa
     7621 ctggtgaaaa aacaactcaa tacctttcat atacctgcat tattattttt cggacgaaga
     7681 gatgaagtaa taccgtacac ggtaggcgaa caatttgccg aaggggtggc gcttgcaaaa
     7741 ttgaccgtgt tagagcgcgg acattatttt attgatgaga agatcaatcc tatgttggaa
     7801 gaagctatgc atttcatttg aagattacgt aggtctgatg atataaacta catttgtact
     7861 gcatgctatt tatcaccatt ggcaccattt tacttattgc aggctacgtt tcactcgtgg
     7921 cattttttct ttatggatgg tggaagttaa aggaatttaa cggcactgct acaaaaaagc
     7981 atgtatttat tacggtaatt gtgcctgtca ggaatgagga aaaaaatatc gtacacttac
     8041 ttcgcgatct gaaggagcaa acctatcctg cagacaaata tgaagtgatc atagtagacg
     8101 atcattctac agatcagaca acacagaaag tgatgcaggc gaaaatggat aatgtgcgca
     8161 ttatttcatt gggagatgcg ggaactgtgc gcatgaacaa aaaagcaggc attcaacatg
     8221 ctgtttcatc ggcaaaaggt gaattgatta ttacaacgga tgctgattgt cacgttggca
     8281 ataaatggat ctctactatt gcggcatatt ataccgaaca tgaacctgta atgatcgccg
     8341 gtttggtaaa ctattttcct gatgcaacat tttttggaaa atttcaaacc cttgattttt
     8401 taagtctcgt gggtattgga gctgcctgca ttcaaaacgg gttttacaat ttatgtaatg
     8461 gtgcgaactt agcatataca aaggcagcat tcgaggcagt gaatggctac gaaggtgttg
     8521 atcatattcc cagcggcgat gatatgatgc ttatgcataa aatggcgcgg aaatttccgg
     8581 gaaagattgc ctcgctgaaa aataaagaca gcattgttta cacacatacc gaaacagatg
     8641 tagtctcatt ttggcatcaa cgtgttagat ggacgagcaa atcaacacat tatgaagata
     8701 agcgtattac attgatattg agtttcgcct atctgtttaa tttactgatt gtattcaaca
     8761 tggtaatcgg gttttttgtg cccggcatgc taaagctttc aatgtggatg tttctggtga
     8821 aaatttgtat tgatacatta tttacgtatg ccgtaagcag attcttcagg cgcgaaaata
     8881 ttctttggct ttttctgcct atccaggcat tacatgtgtt gtatattata ataattgcac
     8941 cggcaggtgt gtttggtaaa tatcaatgga aaggtcgtcg ccattaattc aatgaaattt
     9001 aaacggaaat tacttagcat actgatattc gttgccgcat ccggggcaat tgcagttctg
     9061 tggaaattac tcgacaccga tatagattgg ttcgtggcga tttacaatag tataaccgca
     9121 gttatggtta tttatgttgg cacattaatg ttatttattt acccgattgc ctttcttagg
     9181 aaatactggg agaaaaagaa atcgcagata gacctgaatg cgaaaccgaa atataaagtg
     9241 tacgacaggt tgatgcgcaa tacaaatgca cttgcaggag gtttgtttat tctatgggta
     9301 gccttagctg caatatttgc acatatcatt atcccggata aatcgccata cgcaggagat
     9361 cagatgaatg agattgcgta taaagagcca ggattttctg tgaaggtgtt attagttcca
     9421 gccgacagac acgtttcatc gacaggcgtg atcgattggt ttttgaatgg caagacttca
     9481 tcgtttaaac aaataccaat caatgatgca tggttggtga agggcgactc actttatgtt
     9541 tcgcggtatc ttggagataa tatggttggc attcccatgg tgtttgccac tgaatttatc
     9601 acaggaaaaa aatctgtggt agccgagcaa agccaaataa ttgagaatga atttatcgaa
     9661 acaaggcatt tctggttggg gaccgatgat ttgggcagag attatttaag tagaattatt
     9721 cttggtgcca gggtgtcatt atcagtgggc attattgcgg tactgatcgc attatttatc
     9781 ggtataccac ttggtgcgct ggccggattt tataaacagt atcctccgaa attacgtttt
     9841 gggaaaaagc gtgcagtaaa agttcctgta gacaatagtg tgatgtggat gataaatatt
     9901 gtttggtcta ttcccacatt actgttagtg tttccaattg tttttgcatt cggtcaacag
     9961 tattatacta tttttattgc cgttggtatt accatgtggg tagatattgc gcgcatggtt
    10021 cgcgggcagg taatgcaagt gcgtgaaatg gagtttgtgc aggcagcgcg cacatttgga
    10081 tttagtgata cgagaacgat ctggcgacac gtattaccga atattaccgg tccggttata
    10141 gttgtaacgg ctgccaactt tgcttatgcc attcttaccg aggcgggatt gagttttctt
    10201 ggtattggtg tgcaaccacc cgcaccgagt tggggattga tgattgccaa atacaaggat
    10261 aacctgatta cagatccata tttacccttg atacccggat ttgccatcac cctgctagtg
    10321 cttgcatttt ttatggtggg caacggattg cgtgatgcac tggatataaa aagcaggctg
    10381 taagcagttg ttggaagcaa aatatttcaa ttgtgcagac actttctctt tcggtaacaa
    10441 ttatgtgacc attcatttac acttcaatac acctcatatc ttacttttat ttgcttattt
    10501 tgcatggtcg attgatcaat catggcgata gcaggactta atgtaatgga aatgttgcgc
    10561 aaccagttga agttaaaaca actggagata gattcgctat tggagatcac gaagtcgatc
    10621 aatgcaaata aatcggcatc tgctctgttt gaactttatc accgcacgtt aagagatcag
    10681 attggcgttg aaaatttatt gctcttcagt aatatgggtg gcgattggga agcgatgata
    10741 ggcgataagc tgatttctga agaccacccg gaagtgatgc agtatttgct gccctatgaa
    10801 aaacttactg atttaactac cgtagatatt cctgactacc tcagtgaata tgacttgttg
    10861 attccggtgt tgcataaaga caaaccgctg gcttatttac tcatgagcaa tccacgggtt
    10921 gaaagttatg agccgcttga agacaaaatt aaattcgcac aaacggtaac caatattgtt
    10981 atcgtagcca ttgagaacaa gcgattgttc aagcgcgaag tagaacagca ggcattcaag
    11041 cgcgaactcg aactagccgc tcaggtgcag agtatgctca ttcccgatac ccttccgcat
    11101 accgaacaag tgcagatggc agccatttat ctccctcaca aaaacgttgg aggcgattat
    11161 tatgattgcc ttgagttaaa caataaggaa atatttttct gtattgccga tatttcaggg
    11221 aaaggaattg cagctgctat attgatggca aattttcagg cacaaattcg ggagctcact
    11281 aaacgcaatc cgccaaccat cgaagagtat atgcaggaat tgaacaaagg tgtgttgatg
    11341 agcactaaag gtgagaaatt catcacgctg ttcctggcaa aatatgatcg cactacgggt
    11401 tatctcaaat atgtgaactc cgggcataat ccgccaatat tagttaatag tcaagggacg
    11461 aaattgttgg ataaaggcac aacaatactc ggtatgttcg acactcttcc ttcagtgaaa
    11521 atggggcaat tgccgattac ggaagacaca gcaattgttt gctataccga tggattgact
    11581 gatgtggtaa atgagttcaa tcaggtgttc ccacttgatg aattaattga gttggcaaaa
    11641 caacattata aagaacctgt agaggtgtta aataaaagca tcatcgacga gatcatgcat
    11701 ttcaaaggcc atgacggtga agtgagtgat gatatcactg tgctctcggt aaaaattcat
    11761 cccgcaaatt aatctttagc cggagcttca gcgcttaagg caggatttct tctaagtaat
    11821 aaatcgaaac ttaccagtag cgataggaac atgaaaaatg atgtacctat tttatctgct
    11881 tccaccagat cgctcaatgc caggtttata atgatggtaa tgatcgacaa ggttatcgac
    11941 atggaaatat gtttatactc tatgttggta aatcgatgat acaatcgctg gcctaaatac
    12001 atcgtataga ttaatagtac tacccaaaca aaagtgccgg gaaaaccctg ttcggtggtt
    12061 acctgcaaat aataattgtg cacagttgat cgctccgggt ttttactgat atacgtttcg
    12121 aatttattta cggtatattc tttatagttg aaataaaact ggccgggacc aaatcccata
    12181 taaggcttgt cttctatcat gtgaatggcg gctacccaac gatagatgcg ttcggcactg
    12241 gaaacatcct ccaacgacat ggtagattcc atgtgctcat tgaaattggt atgataaatc
    12301 gtttttttgt aatcaggagc ataatccaaa tagcgattat tatgcaacat gactattgca
    12361 aaaatgataa cgccgaacac cgcaaatact gcacttgtta ctaatttatt cattcttatg
    12421 atgaaaaatg caattgtagc acctgcaacg cttaaccagg atgatcgagt ataagagaaa
    12481 taaatcccgg cgataagcac aactatgcca aattgaatga tccatcgctg ccaggtattt
    12541 ttgggatacc atctgtatgc tgaaaagagc attggaagca tcaaagcaag aaatactgca
    12601 taattcacgt ggttccggaa gaaaggaacc attgttttgt ttacttcgct gaaagcaaaa
    12661 tgatacttat tgtgattgat gagggtgtaa atggtcagcg caagtgtcgg gacaaacaga
    12721 caccagaaag cttttttaaa ggtggcgata tcttttatca ccagcgaggt catgaaataa
    12781 aacgtagtaa tataccatgt cttcgccagc atatatttca aagaaataac aatatcctct
    12841 gaaaacatgg tggtaacaag catccaggca aagtgcacgt ataatattat cgtcaatgga
    12901 tgcaaaaaga atcccgtatc aaacaattca cgtttcaaaa aaatgaaaaa aataaacgtg
    12961 aacataaaaa tgatcatcat gggctcagta ggtaatgtgg tactgaatcc cgacgatgaa
    13021 taatattcaa tggaaaaggg tgtagcaatc aggagcagaa aaaaaatcac tctgaagtct
    13081 acaatcattt ggtaggcaaa cagaaacgca acaggaactg caaaatacaa tggttcctct
    13141 aaaatcacac cggcgaaaat cgcaacaatg gcgagcaatg ttgcaatcca gtatatagaa
    13201 aactcaactg gtttttccag cgttttaagc attttgaatg cgagatttga tctttctgta
    13261 gttttctgta attactgcga gcaaaatcat gaaaacgata gtgccaagtg ttacaccgat
    13321 cacgatcaac gaacgaacgg gtttgtcctt gcgtaaagca gggtaggcgg cctctgtaat
    13381 atgcacagtg ctgaaatcgg tattcaatgt agtttcgttc tgctccaata atttgcgatc
    13441 aagattaagg tcgtcaacgg cgctttgtaa tttgctgtcg agaacaatca tcatttccgc
    13501 tgatgcacct gctttgcgct gcgcagcgat gctatcggta agattagtaa caattaattc
    13561 ttttttggct acctgatttt taaatgtatt tacaataagc tttttatcgc gcataataat
    13621 gtctttatta tgctgatcta ttgtttcaac aatatggttc gcaatatgca ttgcggtatc
    13681 cttattctta tcccaaactg aaacttctat cgcaccatat tctgtttcta ctgcactata
    13741 gttctttaaa aattctttct tgcaattgta gcgcgccatt tctttggtag aatcgatatc
    13801 ataatgctcc atcaaatgga agtgattgat cacataatcg attaagctgc tggtattggc
    13861 gatggtaaga atgcgatcta cgtcactggc agtaccgaaa taatcaattt cgatttcgcc
    13921 ggtactctga ctaaacaata cctgcctgtc ggtcatgatg ggattcgaag gataaaagtt
    13981 taccgtcgac ttaaaatagt cgggcataaa gaaccacgta atgatacagg ctgcgattgc
    14041 cacaaccacc gttgtaatga tgatgggttt acgccattta aaaatgatct gcaataacct
    14101 gatcaggtcg ttcctttgtt ccatacatga agtttagggt gcaaaggtag gaaaatctgc
    14161 ctaagagcct tgcctgcgtg ctattgccga ctgcatcact gttttgaggc tccggacctc
    14221 agtcatattg atgatacctg cgggtatggc gaggaaggcg ccggatgcga gcccaatgac
    14281 ccattgccat gtccaggaaa tattaaaaac ggcaataagc aggttcacac caattaccgc
    14341 tgctagataa aatatcgacc gcagccattt ggagagattt gagcccagcg aaaacaagcg
    14401 atgagccacg gcgatatgtg ctaataatat caatacctga gcaaaaactg tagcctttgc
    14461 tgaacctagc gcttgttgtt cgggaatcag catcaggttt aatatgatat taagtatgaa
    14521 ccccccaaaa ctgatataat tcagcatttt gatactgcca tgtgcggtga tgagactgcc
    14581 atatacataa gtaaggctta tgcaaatgaa acttgccatc aaccagccaa aaatgctgcc
    14641 tgcagagggg tcatgcacat gataaagcaa ctgcatgatg tcttcacgat gtatcagaca
    14701 agcgatgcct gctgaaatac tgcaaatcaa catgatctga aacccgaggt ccagtaaagg
    14761 ttttacggat gtgttttggg atatcatgcg ggcaaacatt ggcatcaaca tccccgccat
    14821 taaaaagccg agtatgctca tggcatccaa caatcgaaat gcggaggcgt agatgcctgc
    14881 ctgataagga cccttatcgc ccaacatccg ctccagcatc actccgtcgg tacggtaata
    14941 ggctgtcatc agaaacccga gtaaggcata aggcaagctt cccttgaaaa tgtttttgat
    15001 cagtgctttg tcgatgcggt ttgaaatggt aagcttttgt ctcgaaagca ctgcaacggc
    15061 aaccagtatg gccacaacaa agcctcctat ctgtatccaa atgaataatg gaattgacat
    15121 ggttatgcct gatgcacccc aaaatatcca ggcacataaa atggatgtgg tgagtttgtc
    15181 cagtatagac aatacagcat ctgtgcggaa caaatgcaaa gcattgacat tgctgcgcaa
    15241 gaaggtgtaa aatgacaagg cgatctgcat caacaccagt agtccgagaa tttgaaattg
    15301 ttcgcctgaa aattgcacgc caaaagcagc gataaatact attatggtat ataccactcc
    15361 taacaatagc ttcagcacca atgtcgacat gatgttacga cccaatgttt gaggatcttg
    15421 agctacctgg cgattgttgt aattgttgat gccgaaatca agcaccacct gaaaaagata
    15481 actgaaattg aacagagcaa aataaacacc ataaacctcc ggacccagcg tattctgcac
    15541 ggtcctgtcg atgcccaata tccaaaatgg cttcaccagc aggttcagaa agagtaaaaa
    15601 gccgatgttg gtgataaaat ttttgcgcat ttttttgttg aagtgttaaa aatagctatt
    15661 attgcttcaa attctacgct agcggtcaac atctactcta aagtttcgtg tctcgactaa
    15721 cctctacgct ttttctgttt attggtaaaa acccaaatgt ataacatgct gatagctgtt
    15781 aatacccgtt tccttattaa ggataaactg gaaggcatag gatggtttac atatgagacc
    15841 atgaaacgcc tcacccggaa ccacccggag catcattttc tgtttctgtt cgacagagac
    15901 tgggatcctg aatttatttt cagcgataac attacccctg tcaaggtatg gcctccggca
    15961 agacatccgt atttatggaa atattggttt gatactgccc tgcctcgtat atttaaaaag
    16021 tacaaacccg atctgtttat ttctaccgat ggcatgatgt ctttagcggt gaatgtgcca
    16081 acagtattgg tggtgcatga tcttgcttat attgaatttc cacatcatat cccacgactc
    16141 acgtacgaat acttaaaaca ttttacgcct aagtatcttt caaaagcaga tgccattata
    16201 acggtatccg attattcgaa aaaggatatc atagataaat atcaaatcgc agatagtaaa
    16261 attgatgttg tggctaatgg agcgagcgat aattttaaac cactggatga agtcaatcgt
    16321 tcaattgtga aacaaaagat cagtagtggt agcgaatatt ttatttatgt tggtagcata
    16381 caccctcgca agaatgtagg catgcttatg aaagcgtatg atcaactgag ggcagaaggt
    16441 atctgtgatc ataaattagt gatcgttggt agatatgcat ggaaatcagg tgaaacttat
    16501 aacatctata agtctatgca atttattgat gatgttatct ttacaggctc aatgaatcag
    16561 gaacaattga cggaagcaat cggtgcggca gacgcattag tgtatccatc cttatttgaa
    16621 ggattcggga tacctgtgtt ggaagcaaga tatgcaggtg tgcctgttat ttgttcaaac
    16681 agtagcagtt taccggaagt gggaggggaa catgccattt attttgatcc caattccgca
    16741 gaggccatca caacggctat ggtccaattt cttaaaaaca gagccgaatt gaaaagaaat
    16801 gcactggaat cagcctatga ggcaagaaat atatataact gggataattc agcaaataag
    16861 atttacagca taatgcaagc cacgtatgac aactatagag caaatagaaa atatactgca
    16921 aatacctgat acggataaga agaccccggt tcgcgttgag gaggccaaat ttatttatca
    16981 gtttatttta ggtcataaga ttcacaaaac gcttgaaatc gggttgggtt atggtcgttc
    17041 tgcttcgtat atcatgctgg ctacaggaaa gccacatatc gctattgatc catttcaatc
    17101 ccattatggc taccaggcta ttaaaaatat cacagatgcc ggcttaaagg acaacttcac
    17161 acatattgaa gatttttctc attttgccct tccggaacta tgtcacaaaa acgaaaaatt
    17221 tgattttgtt tttattgatg gagatcataa attcgatggt atccttattg attactattt
    17281 tgcatcaatg ttagtgaaaa caggaggcta tatcctcttt catgatacat ggatgcgatc
    17341 aacaaggctt gtaaataagt ttgttgaaca aaatcgccat gattttaaaa cacatccggt
    17401 atccctagcc aacctgcatc tcattcagaa gatacaggat aatgatacaa gggacggtat
    17461 gtacttccgc gaattctata caacaaaatc gtggttgaaa tatcggctaa tcacccacct
    17521 taatgatcat cctaaaggtc ttctgagtaa actcaaaagt atttttagac cttaaattta
    17581 ttactatgaa aaagcttttt tggttgacag cattatgtat gctgttaaca caggtttcta
    17641 ctgcccaaat ttatgatatc tattctgtga tccccacatt caacaccgta gaaacagaca
    17701 actattgctc agacagtcct attgctgtgg aagtagttat gacggcagga gccataacag
    17761 atgatgatgg atttagtatt cgcatgaaat catatccggg aggtctaagt tgggcaggtt
    17821 atgatggtct ttgcccggat gattataatt gtgcttatgg tttttctatc gaccctggtg
    17881 atgtaccgga tggcttgtac tattttgaag cttcatgtaa attttttgcc catgccggcg
    17941 acccggatta tgagcagtct gaagtgttca gtcttaaaac aattacatgt ggcaatgttg
    18001 aagacccatt gaattggact tctgcaaaag ttcgtgataa tacaggcatg tccatggccg
    18061 atggtaagat atattatgtg gatgataacc gtaaagtacg taatttttat tggacaggta
    18121 caaattggat ctctgatgtg cttaatacta cagcccctaa ggccaaggat ggtgttgaca
    18181 tcgtggcaac agtggaaaat gtgtattatg taggagatga taataagatc aaaaacctat
    18241 attggacagg cactgattgg gcttatactt cgttagttgc atcgcaacct gaggtgagaa
    18301 gtaattcaaa tattgccttc ggcgatggaa agatctattt tgtatcagct gataataaca
    18361 aagtatacaa cttgtacaag gttggtgcaa catggtattg cagtgcattg aacaatgacg
    18421 ctcctgatgt tcgagatgat agtaagttag caactgatgt aggtaaagta tacttcgttt
    18481 cttcaacacc tttgaatgca tctaccaata aaatgatcta taattattat tgggatggtg
    18541 attcttggga gtatgccatg ttgaatagtt cggcgccaaa ggcaatggat ggcgcaggac
    18601 ctgcagtttg taacgcaaaa gtgtattata ctgcagagga ccaaaaagta aaaaggttga
    18661 aatggaatac cgcaacaggc gtgtgggatg tgaattctct attggctacc caaaagctag
    18721 ttgatgctgg tgagcaaatc gcagttgtgg acgatatcat cttcttccat aactcatttg
    18781 atcttgttta ttacctgaaa gtaagcggtc acgcatggaa tgaatactca atgagcccat
    18841 gcaacgccta ttcaagcacg tcgtattcgg atatcagaga taatacaact attattcctg
    18901 agggaaccgg taaagtttat tatgttggta cagacagaag gttgcgaaat ttctacacag
    18961 tcgcctgcag gtcttccatt gagatctcac cggtaccgac cgaaatatta acggcatttc
    19021 ctaacccatc aaacggtatt attaagttga tgaatgcatc cactgagcca ttatcgataa
    19081 acacactcag ggtcgttgac ttgcaaggca agatggtgta tgtagatacg gaatctaaac
    19141 ttgtaaatgc aaatgcctcc ttagatttgg atctgtctca gatacccaac ggtgtatatt
    19201 tcattttatc tgatgcaggt gatgaaatta tatccaacag gattattatt tcccattaat
    19261 gaggttttct tggtggtata ccaaaaagtg atgtgatctt tatttcatcg ttcgattttg
    19321 atacaacaaa cttaggtggg gagtccgatt atgggctccc cacttttttg tgccactgct
    19381 aaatttacta tctttgacta tggtaattcg catgcttttt tctaagatag ggctctatgt
    19441 atcagcactg ggagcttgtc attgctccat cgatatggcc tattcgcaaa aagtagataa
    19501 tgatcacaga gatcaatacg ctccttctgc agtggtgatc atgcatgaga taaagcaaga
    19561 ttataatcct atctggtatt ccactgaaat gccggctccg ggttcgaact cttatcgtcg
    19621 acacctgatt gatttgaagg atgaattata ccgtaactac atgcctcaac aacaaagcaa
    19681 tttgttgcgt gaagaaacag acatcacttc accggaaata ttaagtggtt gggaaggaaa
    19741 tctgatcacc agctatgttc ccaatgataa cgatctggca atctcgaacg gcggcatgat
    19801 cgtgtctgta atcaactcca atatttatat ttatgatgat gcaggaacag gattattcgt
    19861 tacctctctg cagtcatttg ctgataccct tggcatctta gattctaaat tcgaccctaa
    19921 ggtgatgtac gatccgcgtg cagataaatt tgtgttgtta tttcttgcag gtttcgatcc
    19981 tgaacatacg catgtcatcc ttgcttttag tcaaacaaat gatcctctcg gtgcatggaa
    20041 tttatattcc ttgccgggaa atccgaagga taataatcgg tggactgatt ttccgatgtt
    20101 cgcattgaca gaaaatgaat tatttctcac cattaacctc atcattcccg atgagccttg
    20161 gcaaacagga tttagtgaaa cgttgatttg gcaaatgtcg ctcgacaaag gctatgccgg
    20221 cgatacactt gatgccgtgt attatgacag catttttttt gaaggcaaac cattgcgcaa
    20281 tctcaatcct gtaaaaggcg gcgcaaatac atacggaccg gatatgtttt tcctgagcaa
    20341 tagaaatttc gatcctgcaa atgacactat ttttatcgtg catgttaccg gtactttaga
    20401 tgatccggaa actgaactga cagtagattt tggaagaagt aatattgctt acggtgttcc
    20461 gcctcaggcg cgacaatttt cgacgcatat ttttgatacg aatgacggtc gcgtgcttgg
    20521 aagtttttat gaaaacaacc aaattcaatt tgtgtccaac accctcgatc cggcaaccgg
    20581 attttgcggt gtttaccacg gcgtaatcac tgatcctaca ggggcaaaaa ctataaccgc
    20641 aaatatcatt ggtgatgaca ccctggatct cggatatccg aatatttctt actccggaaa
    20701 atttaccggc gatgatcaat cgattatcac ctgcgagcat acagcgccaa cagtttatgc
    20761 aggggtaagc ggaatatttt ttaattgggg cggttattca gatttaatta atatccatac
    20821 cggtgatacc tacgtgaata ttctcagcgg agcatacgaa cgctggggcg attacaccgg
    20881 atcgcaacgc aaatacgatg aacctggagt agtgtggatg agcggaactt ttggaaaata
    20941 tattgatgta tttccttttg tgcaacgtaa taatgctaca tggattgcat ctctgcgtag
    21001 tacggttgag cctgcagtta atgttcctgt aaattcaaat atgtcacccg caagattatt
    21061 tccaaatcca acagcagatg tgtttactac ggagatcaat ttgagtattg caggtattat
    21121 tgaatgttac ttgtatgata tgcagggccg ccttgttaag tcactgcttc gcgctgaagg
    21181 aaaagccggt aacaatcatt ttcagttcag catggcacca ctatccaccg gaacatatca
    21241 actatacatt accctcaata gtactgctct tactaccctc cccatcataa aaaactaatc
    21301 ccccaaaaaa actctgcgtc tctgcgtctc tgcgtgaacc aaacattgtc atcatggccc
    21361 gtaacaaaaa gaaaatccat
//
LOCUS       DAOQOU010000040        21250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1201_length_21250_cov_5.707950, whole genome shotgun sequence.
ACCESSION   DAOQOU010000040 DAOQOU010000000
VERSION     DAOQOU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1201_length_21250_cov_5.707950"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PLM90_05390"
     CDS             complement(<1..1341)
                     /locus_tag="PLM90_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HMZ88836.1"
                     /translation="MRKVLFLSLAIVAITTNAAAQSWLTAGNTLTGTEKFGSTNNKSL
                     DIVTNNTVRMKINNKGNIGMGITTLGVNDRLSVKITAPVPTATYTMTRGIFAEVTGNA
                     TATFNSPTVAIYGKSNNNNDWGYGGYFEGDLYGAYGKGKYGFFGYGTGASFTGDDLAV
                     GVKGLAEGDDIINYGVYGTAPSDASAYNYGVAAYAGGSFFGNYALLAVDELGGPGAAG
                     FFDGDIEYTGSIFDVSDRQFKTNIKDLDNALSRINQLQPKSYDMKADEFGGLTFGRND
                     EMGFIAQEVQEVFPELVKSCVSPVDPYTMGGQPSKTEPFEFLAVNYVGLIPVLTKGIQ
                     EQQAMINEQANVIKTQEAVIADLVARVSKLETKQNSNIEAVIDGNNARLEQNTPNPFE
                     GTTAISYFIPEYTSSAKLIISDMTGKEIKSIDINTIGDGTIQLTAGALSAGNYTY"
     gene            1654..3555
                     /gene="dnaK"
                     /locus_tag="PLM90_05395"
     CDS             1654..3555
                     /gene="dnaK"
                     /locus_tag="PLM90_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMZ88837.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNDEGRRTTPSIVAFLE
                     NGERKVGDPAKRQAITNPRKTIQSIKRFMGHRYSEVSNELSMISYEVTRGDNDTARVK
                     IGDRLYTPQEISAMVLQKMKKTAEDYLGTEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVKRIINEPTAAALAYGLDKRHKDMKIAVFDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDKKIMDWLADEFKKDEPTVDLRKDPMALQRLKEAAEKAKIELSSSQETEI
                     NLPYITAIDGVPKHLVKKISRSKFEQLSDDLIQRTLRPCEAALRDAGYNKSDIDEVIL
                     VGGSTRIPKIQEVVEQFFGKKPNRGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPL
                     SLGIETLGGVFTRLIESNTTIPSKKSEVFSTAADNQNQVEIHILQGERSMAKDNRTIG
                     RFILDGIPPARRGTPQIEVIFDIDANGILHVTAKDKGTGKEQSIRIEASTGLSDDEIK
                     RMRDEAKANEDADKQSRERIEKLNRADGMIFETEKQLNEYGSKLSDATKAPIEAALNK
                     LREAHKAQDPEGCEVATNELNAAWQNASQEIYAAQQNAGANPDPSAGAADNADNVNKT
                     DDVEFEEVK"
     gene            3972..5429
                     /locus_tag="PLM90_05400"
     CDS             3972..5429
                     /locus_tag="PLM90_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88838.1"
                     /translation="MRIPIFIVLSCSLRMLIGQNWQPFTTSETFSYQSDTAAAIDIGY
                     RVDSFSVAGTDTFYYLNTVIEPLGGDLYLKYGPQFWQKAMRNAGDGLFYFTDPASMAL
                     YTRGAVGDTWEFNFDDHTYATIIAADTQTVLGELDSIKIIETTDGYFITLSKAHGLIR
                     FPVAPGIAPAYCSLSGIRERNLGNFFLGFKDYFDVHVGDVFEYHYSYFFDVWTAKHRN
                     IQYTITAADVSDTLAVFSMEGKQLIAEGIVDPISTLELIDTTIEIRPADHSLTTKLPR
                     AYANMRLTFPFYWYLTESIYDIFQNWGIDSLGNQITGDAYCDIFSPTPYYLFDVDAET
                     AQLYTDYFVSNNFCINYTAGLGLTRVYHYGMESGGSLELTAYVKDGDTVGTLTPDSLY
                     AIAIHESSISKLKLYPNPASEFILISIPQQFETDMRCEVISVAGNRIPITTSRYADKI
                     MADLRSLPPGYYLIKLMDNEGKVWSAPFCKSNSTP"
     gene            5462..5893
                     /locus_tag="PLM90_05405"
     CDS             5462..5893
                     /locus_tag="PLM90_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88839.1"
                     /translation="MMQIGTVAYGTPLYDELVALRYEVLRKPLGLFFTDEQLASEKNY
                     IHFGAYDGEELVGTLQLVPEAHGRMKMRQVAVDPSRQGEGIGALMVEAAEAFARDKDF
                     IIMHCHARASAAHFYERLGYQKTGDMFEEVTIPHWAMEKHL"
     gene            complement(5888..6427)
                     /locus_tag="PLM90_05410"
     CDS             complement(5888..6427)
                     /locus_tag="PLM90_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HMZ88840.1"
                     /translation="MRGLLTLLFLLSGGFMLSSQAQVGRVETGIASYYHDKFVGRKTA
                     SGEIYSQDKMTAAHKSLPLGTWVKVTNLSNDSTVIVKINDRMPKSNGRSIDLTELAAS
                     KLNYLAKGLTKVRIEVIPAPTAVIARPNNLPETPVACIPVKTISVEQRQATIISQPSV
                     KNESFTEVDTSKEKKKRRL"
     gene            complement(6525..6914)
                     /locus_tag="PLM90_05415"
     CDS             complement(6525..6914)
                     /locus_tag="PLM90_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88841.1"
                     /translation="MSKLPAMLKRILFLLLCIGVYTTIHAQSVSNDGVKITPTAEAKI
                     GAGVTLLLSPNPAPKSNPVITVTAVGMDIYSYKVVSATGQIVELENLSGKPDGTIIDL
                     NGAVDIGTYYVIFETNAGKISRKLVII"
     gene            complement(6969..7463)
                     /locus_tag="PLM90_05420"
     CDS             complement(6969..7463)
                     /locus_tag="PLM90_05420"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMZ88842.1"
                     /translation="MIHKKVYITSMQSPLGLLVIKGTDEAIIEIMFADTPLPTDHKIP
                     DVMKQCIVQLEEYFEGTRQSFDIAIAPQGTDFQIQVWELLLRIGYAGTETYAELSKLL
                     GDEKKARAVGNATGKNPIAIVVPCHRLLGSDNSLTGYAGGLWRKQWLLDHEAEHGRGI
                     KKLF"
     gene            complement(7495..7965)
                     /locus_tag="PLM90_05425"
     CDS             complement(7495..7965)
                     /locus_tag="PLM90_05425"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HMZ88843.1"
                     /translation="MYKFISVLLLTIVMNNLRAQAESTISTQFQWMIGTWEISIGEEK
                     IVEVWTSPHPDTLKGYSCLKYGDNTDTLELISITMKNGIIAYGAKVIGENGGEIVYFP
                     FVGYQDGWFVFQNLTHDYPTHIAYMRTDENTLDAYISGNGNSEPARFRYTKSDS"
     gene            8047..9546
                     /locus_tag="PLM90_05430"
     CDS             8047..9546
                     /locus_tag="PLM90_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ88844.1"
                     /translation="MGLKGMLAKPFAAWVARKVRKDAANAIAIQQAVMLNLVRDAAHT
                     AFGKDHGFADIKTHDDFKRQVPVRDYEGLKAYIDRMVSGEKDVLWPGLPLYLAKTSGT
                     TSGVKYIPLTKESIPFHIEAARNALLMYIHETGKTDFVEGKMIFLQGSPALDKKNGIN
                     VGRLSGIVYHFVPQYLQRNRMPSYEINCIEDWEEKVDAIAGVTANERMSLISGIPPWV
                     LMYFERLQQISGKQYIKDVFPDFSLFVYGGVNYEPYRAKIESIIGKKIDAIETYPASE
                     GFIAYQDSQQAHGLLLNVNAGMFFEFIPASEVFNADARRLSLAEVELDVNYAIIINSN
                     AGLWGYSIGDMVKFVSLSPFRIIVSGRIKHFISAFGEHVIGEEVEYAVLTAAADNHVD
                     IVEFTVAPQVNPANGLPYHEWFIEFGKAPADLEAFALQVDNYLQQKNIYYKDLITGHI
                     LRTLVITTVATDGFRNYMKSQGKLGGQNKMPRLSNDRTIADELKQWTVC"
     gene            9606..10925
                     /gene="rseP"
                     /locus_tag="PLM90_05435"
     CDS             9606..10925
                     /gene="rseP"
                     /locus_tag="PLM90_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HMZ88845.1"
                     /translation="MQVLIQVAQLILALTILVFIHELGHFLAARAFGVKVEKFFIFFD
                     AWGKKIWSFKRGDTEYGIGWLPLGGYVKIVGMIDESLDKEQLKNEPQPWELRAKPNWQ
                     KFIVMIAGIVMNVILGVILYIIIHSRYSESYTTTAGVNNDGGIYAAEAGRKYGFETGD
                     KLVSINGKSYERIDDYLSARLYFGSTVIVDRGGKQVEVIVPDSAFRDKMRGKQIFDLR
                     ASIKIDGLSDSLTNARDAGMKKGDIMAMIDAVPVTCFSQMAEMLSAHKNDTAMISVDR
                     GGNMVALAIPVDSMGKIGIETNVKLYPYDSVAYSLGKSIHYGTRDAFEVFYFQAISLW
                     RIFTGDIPAKDSISSPIGISKMFSPTWDWGAFWMLTALLSMVLAFMNLLPIPALDGGH
                     ILFIGIESITGRKLSDKFMERAQVVGMVMLLMLMVFAVGNDILRMIN"
     gene            10929..11756
                     /locus_tag="PLM90_05440"
     CDS             10929..11756
                     /locus_tag="PLM90_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88846.1"
                     /translation="MEKALLYIALLMALPLCSQAQNSGQLLGTDSVVHRVMVIPFNQY
                     NYLSDADPELAKKNEVEAAEISRMFRYGLNYNVSARVISNHGTYNILTDTTVQSKRDL
                     EMIYGSVTYRFEKPMDITTPDTAADHSIHNPDLFGNGGAEPAVEEKKKLSFKKDDEPQ
                     KADKYLNAVVQKPEIFSTLQQRYGADIFIFINQFELVTNYQHCLDRTKNYFERKVVVH
                     YTIYDASGTQLKGDAMTVTFSSEQTNVDEIIATQFPLIADYLSTTVATVTNPPAGNK"
     gene            11753..12277
                     /gene="hscB"
                     /locus_tag="PLM90_05445"
     CDS             11753..12277
                     /gene="hscB"
                     /locus_tag="PLM90_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HMZ88847.1"
                     /translation="MTYFELFNIPVSFLPDETYIRQQYFALSKKYHPDFAATLPIDEQ
                     VQTLEHATLVNKAYQVLSNFERRMQYILQEKGIIEPEGKFNLPPDFLMEMMDLNEEAM
                     QLKMQPDSGRVGQLQQRLQQQDTTLLEAIKPLLEHYNVQSATSDEYASIKEFYYKRRY
                     ILRLKEQINNFARS"
     gene            12308..12392
                     /locus_tag="PLM90_05450"
     tRNA            12308..12392
                     /locus_tag="PLM90_05450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12342..12344,aa:Leu,seq:caa)
     gene            12582..12890
                     /gene="rplU"
                     /locus_tag="PLM90_05455"
     CDS             12582..12890
                     /gene="rplU"
                     /locus_tag="PLM90_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMZ88848.1"
                     /translation="MYAIVNIAGQQFKVEENQEIFVHRQTGNEGDKLEFSEVLLKARD
                     GKVEVGAPTVAKASVSATILSHMKGDKVIIFKKKRRKNYRVKNGHRQSFTKIKIDKIS
                     "
     gene            12912..13172
                     /gene="rpmA"
                     /locus_tag="PLM90_05460"
     CDS             12912..13172
                     /gene="rpmA"
                     /locus_tag="PLM90_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMZ88849.1"
                     /translation="MAHKKGVGSSKNGRESHSKRLGVKVWGGQPVIAGNIIIRQRGTR
                     FHPGAGVGLGKDHTIFALVDGIVTFKKGREDRNIVSVLPAEA"
     gene            13323..13646
                     /locus_tag="PLM90_05465"
     CDS             13323..13646
                     /locus_tag="PLM90_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ88850.1"
                     /translation="MRRDVFQAIADPTRRAIIGLIALQAMTPNALAEHFDMSRQAVSK
                     HLRILTECELVKPEQQGREIYYLLEMEKMKEVDQWLEQFRKIWVARFDELDGVLDRMK
                     KKNKG"
     gene            13679..14089
                     /locus_tag="PLM90_05470"
     CDS             13679..14089
                     /locus_tag="PLM90_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ88851.1"
                     /translation="MATKVNIHATVAAPREKVWACYTEPEHITKWNFAADDWQCPSAS
                     NDMRVGGKYTARMEAKDGSFGFDFEAIYNAIDPGNSFTYTIPDGRVVDVVFSDNGSNT
                     DVAITFDAESVNPVELQQAGWQSILNNFKEYTEG"
     gene            complement(14108..15226)
                     /locus_tag="PLM90_05475"
     CDS             complement(14108..15226)
                     /locus_tag="PLM90_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88852.1"
                     /translation="MRTLLILLRKEFQQIRRNPSILRIIFLMPAVQLLILPLAADYEV
                     KHVNLTIVNNDHSTYAQRLVQKFEVSDHFNITGYVDNYTEALQTIEQDKADIIITIPD
                     HFERDLIREDKSELHLAVNAVNGAKGNIGSAYAMTIINQFNQEVRSEWIVFPKTNPVP
                     IIDVTTINRFNDDMKYSLFMVPGILVILVTMVGSFLASLNIVHEKEVGTIEQLNVSPI
                     KKYQFILGKLIPFWLLGFVVLTIGLLIARLVYGIIPEGSLGLIYLFASVYLLALLGIG
                     LLISTLAETQQQAMLMSFFFMMVFILMGGLYTSIDGMPHWAQVVTRFNPVRYFIEVMR
                     MVIMKGSGLRDILPNLYAIGAMAIFFNTLAILSYRKRS"
     gene            complement(15223..16380)
                     /locus_tag="PLM90_05480"
     CDS             complement(15223..16380)
                     /locus_tag="PLM90_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ88853.1"
                     /translation="MPSLLNWRVVLKEPPTDMRQYGVFIKKEFKHIIRDKRSLLILFA
                     LPIAQILIFGFALTNEIKNTKIAILDLSQDEASHQLRDQLIASQYFELNGDVHDVADV
                     ESALLSDQIKLAVIIPENFQNDLSHGNEAQVQLIADATDPNTATTLSVYASAVIRDFQ
                     GDINGAYNLPYTITPEVRMLYNPQLKGAYLFVPGVMGMILLLISAMMTSIAIVREKEI
                     NTMEIILASPLKPIVLILSKAVPYITISLVNVTTILLISIFILGLPVAGNIFLLFAVC
                     FLFITTAIALGLMISNITDSQQTAMFISLMGLMLPTLILSGFMFPIENMPVPMQVLSN
                     IVPTKWFFIIVKDVMIKGLGFASIWREVLILTGMTLFFLFVSYKKFKTRLA"
     gene            complement(16331..17083)
                     /locus_tag="PLM90_05485"
     CDS             complement(16331..17083)
                     /locus_tag="PLM90_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ88854.1"
                     /translation="MHKEGQIVISASELTKTFGDFTAVDHISFEVKKGEIFGFLGANG
                     AGKTTAMKMLIGILSPTSGNASVAGYDIYTQTEHVKQNIGYMSQRFSLYEDIPVIENI
                     RFFGGIYGVGAKELKAKSAALLDKLELTSSAKTLVRDLPLGWKQKLAFSISILHDPQV
                     VFLDEPTGGVDPITRRKFWELIYEAADRGVTVFVTTHYMDEAEYCDRISIMVDGRIAA
                     MDKPAILKEQFQSPTIDAIFTQLARGAKRTAD"
     gene            complement(17076..17993)
                     /locus_tag="PLM90_05490"
     CDS             complement(17076..17993)
                     /locus_tag="PLM90_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ88855.1"
                     /translation="MESAIHIHNLHKTFNKGKLVALDNINFDVRKGELFGLIGPDGAG
                     KTSLFRILTTLWLADSGNATVAGLDVVKDFKEIRRRIGYMPGKFSLYQDLSVEENLNF
                     FATLFNTSIEENYDLVKDIYVQLEPFKSRKAGKLSGGMKQKLALCCALIHKPEVLFLD
                     EPTTGVDPVSRKEFWEMLKRLQSRITIIVSTPYMDEATLCDRIALMQGGKIMQLDTPQ
                     QIVDRFADELIAVRSEQMYHLIRDIRELPDIKGCYGFGDYLHVRFAHGAWTQERLTVA
                     LKDRHTLLEFCPITPGIEDCFMALMESHA"
     gene            complement(17999..18889)
                     /locus_tag="PLM90_05495"
     CDS             complement(17999..18889)
                     /locus_tag="PLM90_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ88856.1"
                     /translation="MQRIIIIAGLSMLLGACSNGDKIYDATGTFEATEVIVSAGTAGK
                     LLALNVEEGQSLEANAVAAIVDTTQLHLKKAQLNAQIQAVMSREPNIPTQIATIQEQI
                     RVAAGEKQRMEILVKAGAAPSKQLDDIVNQIAVLEKQLDAQRTSLMTTAGSIYAEVNP
                     LTLQIAQIDDQLVQSRVLNPIKGTVLTKYAETGEYTAPGKPVYKIADLTEMQLRAYVT
                     GDQLPGIKIGQQVKVLVDSADGQYSEHAGTVSWISDKAEFTPKSIMTKDERANLVYAI
                     KITVPNTGNIKIGMYGEVVF"
     gene            complement(18896..20152)
                     /locus_tag="PLM90_05500"
     CDS             complement(18896..20152)
                     /locus_tag="PLM90_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ88857.1"
                     /translation="MKRFFLIILLLPLTLRAQQSVTLADCQRAAEANYPLLGQSALLQ
                     EITDANIQKINAIWQPQVYVNAQATYQSEVTSLPISFPGIEVPELSKDQYKATIDISQ
                     TLYDGGISSQQKAIAGQNLAVEMQKVEVDKFKIRDKVDQVYMSILMADARIELMVIAV
                     SDLNTVLSISKVGGTNGVVSDQQILILQAEIMKTEQQLTELKALRDGAIRMMAVLTAL
                     PLTSTSVFARPEPAFEGTAMNNQRPELQLYAQQRTLAGLQQQYAEAATLPKVSLFAQG
                     GYGKPGLNFLVDSFSTFYMGGIKVSYPLWTGDTKRRDAKIFGLNSEIIGMQQLQFEQS
                     INVQSAQYQSDIDKYKTLISQDDSLVNLRTSILTMARVQLEQGVISTKDYILYAKDLD
                     DARINRSIHDLQLLATKVNYLNTLGK"
     gene            complement(20149..20784)
                     /locus_tag="PLM90_05505"
     CDS             complement(20149..20784)
                     /locus_tag="PLM90_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ88858.1"
                     /translation="MSKTQNTTPDRNTEEAILQAAKRVFTRKGFAAARMEDIATEAGI
                     NRALLHYYFRSKEKMFERVFEENMSHFYGSFISILNAPAPLDMRIRNLVNAEIDMLLA
                     NPDVPLFILTEISRNPDMIKSKMEKMPVREFMMIFVSSLRTEMQKGTIRQIEPVHVLM
                     SLMSLCIFPFIGKPMFLTVSGMPPEQFESLMQSRKKVVADIILQMLKPEQA"
     gene            complement(21047..>21250)
                     /locus_tag="PLM90_05510"
     CDS             complement(21047..>21250)
                     /locus_tag="PLM90_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88859.1"
                     /translation="DQFVRDLADRYGAHLSSMYGSGNLYEFRFSNLVIDESWTKKLEA
                     LYHEKGVELVSPSLYSPVVLDGE"
BASE COUNT         6120 a         4470 c         4645 g         6015 t
ORIGIN      
        1 ataggtgtag ttacctgcag aaagcgcacc ggcagtcaat tgtatggtac cgtcgccaat
       61 agtattgata tcaatcgatt tgatctcctt gcctgtcata tcagaaatga tcagtttggc
      121 agatgatgtg tactcaggaa taaagtaact gatggcagtt gtgccttcaa atgggttagg
      181 agtattttgc tctaaacggg cattattgcc atcaatcacc gcctcaatat tgctgttttg
      241 cttcgtttca agcttgctaa cacgtgcaac gaggtcggcg attacagctt cttgcgtttt
      301 gattacatta gcctgttcgt ttatcatcgc ctgttgctct tgaatacctt ttgtaagcac
      361 aggtatcaag ccaacgtaat tcaccgccag gaactcaaat ggctcagttt tactgggttg
      421 tcctcccata gtgtatggat caaccggaga tacgcatgat tttaccaatt caggaaatac
      481 ctcctgcact tcttgagcta taaatcccat ttcatcattt ctaccaaatg tcaaacctcc
      541 aaattcgtcg gccttcatat cataagattt cggctgcaat tggttgattc tgctcagggc
      601 attatctaaa tctttaatat tagttttgaa ttgcctgtca ctcacatcaa aaatagaacc
      661 ggtgtattca atatctccat caaaaaatcc cgccgcaccc ggaccaccaa gttcatcaac
      721 agctaagagg gcgtaattac caaagaatga tcctccggca taagctgcaa cgccataatt
      781 atatgcagag gcatcacttg gggctgtgcc ataaacgcca tagttaatga tatcatcacc
      841 ttccgcaagc ccttttacac ctacagctaa atcatcgcct gtaaaagaag ctcccgtacc
      901 atatccaaaa aaaccatatt tccccttgcc ataggctcca tacagatcac cttcaaaata
      961 gccgccatat ccccagtcgt tattattatt cgattttcca taaatagcaa cggttggact
     1021 attaaatgta gcagtagcat tacctgttac ctcagcaaaa atgcccctag tcatagtgta
     1081 ggttgcggta ggaaccggtg ctgtgatttt tactgataat ctgtcattca cacccagtgt
     1141 agtgatgccc attccgatgt tgcccttatt gttgatcttc atccttacgg tattattggt
     1201 aacgatgtcg agcgacttgt tgttggtaga gccgaatttc tcagtcccgg taagcgtgtt
     1261 tccggccgta agccaggatt gagctgcagc gtttgtggtg atggctacta tcgccagcga
     1321 taagaatagt acttttctca tggttttgtt tgttgttttt gggttaaagt ttgggtttgc
     1381 ttgaattgaa gttacaaagc tatttgcaac ctatgcgtcc ggcaagacaa catttagttg
     1441 aatattctat atcagagcat taaatataca cttaaaatat tgataatcaa taatatgtgt
     1501 aaattctagt aaatcaaatt cgtcagtaaa tttgccttgt tttggacaaa atgtattaga
     1561 attcaatgcc aaatctgtca tgctgtcagt tatttcaaga tggtataaaa attgacctga
     1621 acgcttagaa taaactgaaa ataaccgata aacatgggaa agatcatagg tatagactta
     1681 ggaacaacta acagctgcgt atctgtaatg gaaggaaacg agcctgttgt tatcccgaac
     1741 gatgaaggcc gcagaacaac gccatccatc gttgcatttc ttgagaatgg cgaacgcaaa
     1801 gtaggagatc ctgcaaagcg tcaggccatc acaaatcctc gtaaaacaat tcaatctatc
     1861 aagcgattca tgggtcaccg ctatagcgaa gtgagcaatg aactcagcat gatcagctac
     1921 gaagtaacgc gtggtgataa tgataccgca cgagtaaaaa ttggcgacag gctttatact
     1981 ccgcaggaaa tttctgccat ggtgttgcag aaaatgaaaa agactgctga agattatttg
     2041 ggtaccgaag ttacagaagc tgttatcaca gttccggcat acttcaacga ctctcagcgt
     2101 caggctacca aagaagcagg tgaaatcgcc ggtttgaaag taaaacgtat tatcaatgaa
     2161 cctactgccg cagcgttagc ttacggttta gacaagcgtc ataaagacat gaagatcgcc
     2221 gtattcgatc ttggtggtgg tacttttgat atctctattc ttgaattagg tgaaggcgtg
     2281 tttgaagtga aatctaccaa tggtgataca caccttggtg gcgacgactt cgataaaaag
     2341 atcatggact ggcttgctga tgaattcaaa aaagatgagc ctactgtaga tctccgcaaa
     2401 gaccctatgg ctttacaacg cttgaaagag gcagctgaaa aagcaaagat cgaattatct
     2461 tcttcacaag aaactgaaat caacttacca tatatcacag cgattgacgg cgtgcctaaa
     2521 caccttgtga aaaaaatcag ccgttcgaaa tttgaacagt tgagcgatga tttgattcaa
     2581 cgcacactgc gtccttgtga agctgcattg cgcgatgccg gatacaataa aagtgatatc
     2641 gatgaagtga tccttgttgg tggatctaca cgtatcccta aaattcagga agtagtagaa
     2701 caattcttcg gcaaaaaacc gaatcgcggt gtgaaccccg atgaagtggt tgccgttgga
     2761 gctgcaattc aaggtggtgt gttgaccggt gaagtgaagg atgtgctgtt gctcgacgtt
     2821 actccattgt cgctcggaat tgaaacgctt ggtggtgtat ttacacgatt gattgaatca
     2881 aatactacca ttccttctaa gaaaagcgaa gtgttctcta cagctgctga caaccaaaat
     2941 caagtagaaa tacacatcct tcagggtgaa cgttctatgg cgaaagataa tcgtactatc
     3001 ggaagattta ttctcgatgg tataccacct gcacgtcgcg gaacgccaca aatcgaagtg
     3061 attttcgata tcgatgccaa tggtattttg catgtaactg ctaaagataa aggcaccggt
     3121 aaagaacaaa gtattcgcat cgaagcatca accggtttga gcgatgatga aatcaaacgc
     3181 atgcgcgatg aagcaaaagc taatgaagat gccgataaac aatcacgcga gcgtatcgaa
     3241 aaactgaatc gtgccgatgg tatgatcttc gaaactgaaa agcagttgaa tgagtacggc
     3301 agcaaattaa gcgatgccac caaagcacct atcgaagccg ctctcaataa actgcgagaa
     3361 gcacataaag cacaagatcc ggaaggttgt gaagtagcaa ctaacgaatt gaatgctgca
     3421 tggcaaaatg ccagccagga aatttacgct gctcaacaaa acgccggcgc aaaccctgat
     3481 ccttctgcag gagcagcaga taatgccgac aatgtcaaca aaaccgacga tgttgaattt
     3541 gaagaagtga agtaattgtt cgcataaagt atacataagc ccatttgccg aaaggtagat
     3601 gggctttttt tatgaaatgc ttttcgtgaa cttgcctcgc cgataggcgg gtggcgcccc
     3661 gtcccactac cgatcgtgag tcgtgaatcg tgagtcgtga gtgaggtgcg tactagagaa
     3721 tcgggtacac aatgcgaaca tgcattgata tctgatactt gatacttgat aatcaatacc
     3781 cactaccaac cgtgagtcgt gaatcgtgag tcgtgagtga ggtgcgtact agagaatcgg
     3841 gtacatgatg cgaacatgca ttgatactga tacttgatac ctgatacctg atacctgata
     3901 cctgatactt gatactcaat acccactacc aattaacgac caaactcata aattttcgtt
     3961 atatttgaat tatgcgtatc cccatattca tcgtattgag ttgttcgttg cggatgttga
     4021 tagggcaaaa ctggcaaccc tttacaacct cagaaacatt ttcgtaccaa agcgatactg
     4081 ctgctgcgat tgacatcggt taccgcgtcg attcattcag cgttgcagga actgatactt
     4141 tttactattt aaatacggta atcgaacctc ttggcggaga tttgtatctg aaatacggac
     4201 ctcaattctg gcagaaagct atgcgcaatg ccggtgacgg gcttttttat tttactgatc
     4261 ctgcatctat ggcattatat acccggggag ctgttggcga tacatgggaa tttaattttg
     4321 atgatcatac ctacgcgaca attattgcag ccgatacgca aacggttttg ggtgaattag
     4381 attcaatcaa aataatcgaa accacagacg gatactttat aaccttatca aaggcacatg
     4441 gactaattcg atttcctgtt gctcccggta ttgcaccagc ctattgctca ttatcgggta
     4501 taagagaaag aaacttgggg aacttctttt taggatttaa ggattacttt gatgttcatg
     4561 tcggcgatgt atttgaatat cattattcct atttttttga tgtctggact gcaaaacatc
     4621 ggaatatcca atatacgatc acagctgcgg atgtgtctga tacgcttgct gtcttttcaa
     4681 tggaggggaa acaattgata gcagaaggga ttgttgatcc aatttctaca ttggaattga
     4741 tagacacaac aatcgaaatc agaccggcag atcattcact tactacaaaa cttcctcgtg
     4801 catatgcaaa catgcggctt acctttccgt tttactggta tttgacggaa tcgatttacg
     4861 acatttttca aaactggggc atagattctc taggcaatca aattacgggt gacgcatact
     4921 gcgatatctt ttcgccaacg ccatattact tgtttgatgt ggatgccgaa actgcacagt
     4981 tgtatacaga ttattttgta tcaaataatt tttgcattaa ttatactgca gggttaggac
     5041 tcacccgtgt ttatcactat ggcatggaaa gtggcggatc gctggaattg acagcctacg
     5101 tgaaggatgg tgatacggta ggaacattaa ctcccgatag tctgtatgca attgccatac
     5161 atgaaagcag catttcaaaa cttaaacttt acccgaatcc ggcaagtgaa ttcattttga
     5221 tttcaatacc gcagcaattt gaaaccgata tgcgttgcga agtgatttct gtcgcaggaa
     5281 accgaattcc catcaccacc tcacggtatg ccgataaaat tatggcagac ctgcgttcac
     5341 tccctccggg atattatctt ataaaactga tggataatga aggcaaagta tggagtgcac
     5401 cattctgtaa atcgaattct actccgtagt tttacttagc tactaaacga gatcaatagc
     5461 tatgatgcag atcggtactg ttgcctatgg cactccgctt tatgatgaac tggttgcgct
     5521 gcgatatgaa gtgttgcgca aacctctcgg attatttttt acggatgagc aactggcatc
     5581 cgaaaaaaac tacattcatt ttggagcata tgacggagaa gaattggttg gcaccttgca
     5641 actcgtgcct gaggcacatg gtcgtatgaa aatgcggcag gtagctgttg acccgtcacg
     5701 ccaaggagag gggattggcg cacttatggt agaagcagct gaggcatttg cacgcgacaa
     5761 agatttcatc attatgcact gtcatgcacg cgcatctgca gcccattttt acgagcgatt
     5821 aggttatcaa aaaacaggtg atatgttcga ggaggtaacc atcccgcatt gggcaatgga
     5881 gaaacatcta taagcggcgc ttcttctttt cttttgaagt atccacctca gtgaaggact
     5941 cgtttttaac tgagggttgg gatataatag tagcttgcct ttgttcaaca cttatagttt
     6001 tcacgggaat acaggctaca ggcgtttccg gaagattatt agggcgtgcg ataacagctg
     6061 ttggagcagg aataacttca atacgcacct ttgttaatcc cttagccaga taattcaatt
     6121 tcgaagcagc aagctcagta aggtcgatgg agcgcccgtt gctcttaggc atgcggtcgt
     6181 taattttcac gattactgtg gagtcattac tcaaattggt cacttttacc cacgtaccaa
     6241 gtggcagact tttatgggcg gcggtcattt tatcctgact atagatctct ccgcttgcag
     6301 tttttcgtcc tacaaatttg tcgtggtagt agctggcaat gcctgtttct acgcggccca
     6361 cctgagcctg cgacgaaagc ataaagcctc cggaaagtag aaaaagtaag gttaaaagcc
     6421 cacgcataca acggtttgag gtatccaaat tacctcatac ccctgttaaa ggcaaaaatc
     6481 aggccgcttt gttatccaca atgggccaaa tcgaaatata agtatcagat aatcaccaac
     6541 ttacgactga tctttcctgc gtttgtttca aagatcacgt agtaagttcc tatatcaacg
     6601 gccccgttga ggtcaataat ggtgccgtca ggtttgccgc ttagattctc cagttcaacg
     6661 atttgaccgg tagcggaaac aactttataa gagtagatat ccattccaac ggcagtcaca
     6721 gtaattaccg gattgctttt tggtgccgga ttcggactaa gcaacagcgt tacgcctgct
     6781 cctatctttg cttctgcagt tggggtaatc ttaacaccat cattggaaac agattgtgca
     6841 tggatagtgg tatatacacc tatacaaagg agcaaaaaga ggatacgttt gagcatggct
     6901 ggtagctttg acaataacaa aaataatcga aatttttaat taggcaagcc aacctgtcaa
     6961 atatgacatt agaataactt tttaatgcca cgcccgtgct cagcttcatg gtccaataac
     7021 cactgcttgc gccacaatcc gcctgcatat ccggtaagac tattatcgga gccgagcagt
     7081 cggtgacaag gcaccacaat ggcaattgga tttttacccg tagcattgcc tactgccctt
     7141 gctttctttt catcacccaa caatttcgac aattctgcat aagtctctgt tcctgcatag
     7201 ccaatccgca acagtaattc ccaaacttga atctgaaaat ccgttccttg aggagcaatt
     7261 gcaatatcaa agctttgccg agtgccttca aaatactcct ctaactgaac gatacactgt
     7321 ttcatcacat ctggtatctt gtgatcagtc ggcaaaggag tatctgcaaa catgatctct
     7381 atgatggcct catcggttcc ttttattacc agcaatccca gtggcgactg catggaggtg
     7441 atatagacct tcttatgtat caaggatata tttatttaaa ataaaggtaa gcaatcaact
     7501 atcagatttt gtatatctga agcgtgccgg ctcggaatta ccattaccgg aaatataggc
     7561 atcgagggta ttctcatctg tgcgcatgta agcaatatga gtaggatagt catgcgttaa
     7621 gttttgaaat acaaaccaac catcctgata tccaacaaaa gggaaataaa caatctcgcc
     7681 gccattttcc ccgatcactt ttgcgccata ggcaattatg ccatttttca tggtgatgga
     7741 aattaattca agtgtgtcag tattatcacc atatttaaga caactgtatc ctttcaaagt
     7801 atcaggatgc ggggatgtcc atacctccac aatcttttct tctccgatag agatttccca
     7861 ggtgcctatc atccattgaa attgagtaga gatggtgctt tccgcctgag cccttaggtt
     7921 gttcatcact atggtcagca ataacaccga aataaattta tacatggctt aaagatacat
     7981 gcttttacag ggatgtgaac gacaaagcct attgggtaat tgcacaaaca tgcgcatctt
     8041 tgtgccatgg ggctgaaagg gatgttagcg aaaccgtttg ccgcctgggt agcgcggaaa
     8101 gttcgcaagg atgcagcaaa tgccatcgcc atacaacagg cggtgatgct gaatcttgtg
     8161 cgtgatgcgg cacatacggc atttggaaag gatcatggat ttgccgatat caaaacacac
     8221 gatgacttca aacgccaggt tcctgttcgc gactatgaag gattgaaagc ctatattgat
     8281 aggatggttt caggtgaaaa agatgtactc tggcccggat taccactata tcttgccaag
     8341 acctcaggca ctacatcagg tgtaaaatat atccctctca ccaaagaatc tattccattt
     8401 cacatcgaag cggccagaaa tgcattgttg atgtacattc atgaaaccgg aaaaacggat
     8461 ttcgttgaag ggaaaatgat ctttttacaa ggctcaccgg cgcttgacaa aaaaaatggc
     8521 atcaatgtag ggcgtttatc aggaatcgtt tatcattttg ttcctcaata tctgcaacgg
     8581 aatcggatgc cttcgtatga gatcaattgt attgaagatt gggaagaaaa ggtagatgct
     8641 attgctggag taactgcaaa tgaacgcatg agtctgatca gcggcattcc accatgggtg
     8701 ctgatgtatt tcgaaagatt gcaacagatc tccggaaagc aatatatcaa agatgtgttt
     8761 cccgattttt cattgtttgt atatggtgga gtgaattatg agccatacag agcaaaaatc
     8821 gagtcaatta tcggaaagaa aatagatgcc atcgagactt atcctgccag tgaaggattt
     8881 attgcctatc aggattcaca gcaagcacat ggattgttgc tcaatgtaaa tgcaggtatg
     8941 tttttcgaat ttattcctgc ttcggaagta tttaatgcgg atgcgcgaag actttcactt
     9001 gcagaagtag aactcgacgt aaactacgca atcatcatta acagcaatgc cggactttgg
     9061 ggttatagca ttggcgatat ggtgaaattt gtgagtttat cgcctttccg aattatcgtt
     9121 tccggtagaa taaaacattt tatttcagcc ttcggcgaac atgtgattgg ggaggaagta
     9181 gaatatgccg tattgacagc agcggcagat aaccatgtgg atatagtgga gttcactgta
     9241 gccccgcagg taaacccggc gaacggatta ccttatcacg aatggtttat agaattcggt
     9301 aaggcacctg ccgatttgga ggcatttgca ttgcaggtcg acaactatct gcaacaaaag
     9361 aatatctatt acaaagacct gataacaggg catatattaa ggactctggt gatcacaacg
     9421 gtggcgactg atggtttcag gaactacatg aaaagccagg gtaaactggg aggacaaaac
     9481 aagatgcctc gactcagcaa cgaccgaacc atcgccgatg aactaaaaca atggacagtg
     9541 tgttaacaaa gtttaatctc attagtggtt aaatcccacg tttattatcg aatttcgcag
     9601 gcattatgca agtattgatc caggtagcac agcttatttt agcactcacc atactcgttt
     9661 tcatacatga attagggcac tttttagccg ccagagcctt cggtgtgaag gtggagaaat
     9721 tcttcatttt tttcgatgcc tggggaaaga agatatggtc attcaaacgc ggtgataccg
     9781 aatatggtat cggttggttg cctttaggag gatatgtgaa aattgtgggg atgatagatg
     9841 aaagtctcga caaagagcaa ttgaaaaatg agccgcaacc ctgggaatta cgtgcgaaac
     9901 caaattggca gaagtttatt gtgatgattg ccggtattgt catgaatgtg attcttggtg
     9961 tcatcctgta tattattatt catagtcgct attcagagtc atataccact actgccggtg
    10021 tgaataacga tggtggtatt tatgctgccg aagcaggccg caaatatggg tttgagacag
    10081 gcgataaatt ggtatccatc aatggaaaat cttacgaacg cattgatgac tatttatcag
    10141 ctcgtttgta tttcggatct actgtcattg tagatcgcgg tggcaaacag gttgaagtaa
    10201 tcgtacctga tagtgcgttc agagataaaa tgcgcggcaa gcaaattttt gatttgcggg
    10261 caagtattaa aatcgatgga ctctcggatt cacttaccaa tgcgcgtgat gcgggtatga
    10321 aaaaaggtga tatcatggcg atgatcgatg ctgttcccgt tacttgtttt tcgcagatgg
    10381 ctgaaatgtt atccgctcac aaaaatgata cagccatgat atccgtggat cgcggaggca
    10441 atatggtggc cttggcaatt cctgtcgaca gcatgggaaa aatcggtatc gaaacaaatg
    10501 tgaaattata tccttacgat tcagtggcat attctcttgg caaatcgata cattatggca
    10561 caagagacgc atttgaagta ttttattttc aggccataag cttgtggcgc attttcacag
    10621 gtgatattcc tgcaaaagat tctatcagca gtccgattgg gatttctaaa atgtttagtc
    10681 ctacatggga ctggggtgcc ttctggatgt tgacggcttt attgagtatg gtattggcat
    10741 ttatgaattt gttgccaatt cctgcgcttg atggcgggca tattttattt attggaattg
    10801 agagtattac aggaagaaag ttatcggata aattcatgga gcgcgcgcag gttgtgggaa
    10861 tggtaatgct tttaatgctg atggtgtttg cagttggtaa tgatattctt cgcatgataa
    10921 attaaccgat ggaaaaagcg ctgctatata ttgcgttgtt gatggcattg ccattgtgtt
    10981 cgcaagcgca aaattccggt caattgttag gcacagattc cgtagtgcat agggtgatgg
    11041 taattccatt taaccaatac aattatctct ccgatgcaga ccctgaactg gcgaaaaaga
    11101 atgaagtgga ggcggccgaa atttcgcgca tgttccgtta tggattgaat tataatgtca
    11161 gcgctcgggt gatttcaaac cacggcacct acaatattct taccgatact acagtgcaat
    11221 ccaaacgtga tcttgaaatg atatatgggt cagttaccta tcgttttgag aagccgatgg
    11281 atattaccac tccggatacc gccgcagatc atagtattca caatccggat ttgtttggca
    11341 acggtggtgc tgaacctgcg gtagaggaaa agaagaagtt atcctttaag aaagatgatg
    11401 agccgcaaaa ggccgataaa tatttaaatg cggtagtgca gaagcctgaa attttcagta
    11461 cgctgcagca acggtatggt gccgatattt ttattttcat caatcagttt gaattagtaa
    11521 ctaattatca gcactgcctt gatcgcacaa aaaattactt tgagcgaaaa gttgttgttc
    11581 actataccat ttacgatgct tcaggcactc aattaaaagg cgatgcgatg acagtgacat
    11641 tcagcagtga acaaacaaat gtcgatgaga tcattgcgac ccaatttccg ttgattgcag
    11701 attatttatc gaccacggta gcaaccgtaa ctaacccacc ggcaggcaac aaatgacgta
    11761 cttcgagtta tttaatattc cggtaagttt tcttcccgat gaaacatata tcaggcagca
    11821 atatttcgcg ctgagtaaaa aatatcaccc tgatttcgcg gccaccttgc ccattgatga
    11881 acaagtacaa acccttgagc atgccacgct tgtaaacaaa gcctatcagg tgttgagcaa
    11941 ttttgagcga aggatgcaat atatacttca agaaaagggc atcattgaac ctgagggtaa
    12001 attcaattta ccacctgatt ttctgatgga aatgatggat ttgaacgaag aggccatgca
    12061 actgaaaatg caacctgatt ccggacgagt agggcaattg caacaacgcc ttcaacagca
    12121 ggataccaca cttttggaag caatcaaacc ccttctcgaa cactacaacg tgcaatccgc
    12181 cacctccgat gaatatgcat caatcaagga attttactat aaaagacgat atatattgcg
    12241 attgaaggag caaatcaata actttgcccg ctcctaacac taaaccggac ctcaatctgg
    12301 tttttttgcc gaagtggtgg aactggtaga cacgccggac tcaaaatccg gtgccctcaa
    12361 aaagcgtgcg ggttcgattc ccgccttcgg tacttctcct gcaattttcc tgctgttttt
    12421 cactttaatg cctccgctag ccctaattgc ctgtgttcat accctaattt ttggcgtaac
    12481 taattgatta tcaatagcta ataaattcct taatattaat tttctctatc ctttgtctaa
    12541 attatttgga ttcattatct ttgcaccttc attgaaaagt catgtacgcg atagtaaaca
    12601 tagccggtca gcagttcaag gttgaagaaa accaggagat ttttgtgcat cgccaaacgg
    12661 gaaatgaggg cgataagctg gaattcagcg aagttctgct caaggcccgt gatggtaaag
    12721 tggaagttgg agctcccaca gttgccaaag cgagtgtatc tgctaccatc ctttctcata
    12781 tgaaaggcga taaggtgatc atcttcaaaa agaaacgccg caaaaattac cgagtgaaaa
    12841 acggacatcg tcagtctttc accaagatca aaatcgataa aatctcctaa ttttttaaat
    12901 atttaagcat catggcacat aaaaaaggtg ttggtagttc caagaacggt cgcgaaagtc
    12961 atagcaaacg cctcggcgtt aaagtttggg gaggtcaacc tgtaattgca ggaaacatta
    13021 tcatccgtca gcgtggaaca cgtttccatc cgggagccgg tgtagggctt ggtaaagacc
    13081 acaccatttt cgccctcgtt gatggtatcg taacctttaa aaaaggtcgc gaagatcgca
    13141 atatcgttag cgtattacct gccgaagcat aagctaagcg cacaaccata ttcaaaaccc
    13201 ttgtcggccg acaagggttt ttttgttgcc tgttacttcg aaaaatctcc cgcaaatatt
    13261 tgcgaaattg attggttgca tgtaatttag caaccaaatg gtttcctaaa ttgtccgtta
    13321 ctatgagaag ggatgtattt caagctattg ctgatcctac cagacgtgcc attattggac
    13381 tcattgcttt gcaagccatg acaccaaatg cactggcaga acatttcgat atgtcgaggc
    13441 aagctgtatc caaacacctt cgtatactta cggaatgtga actggtgaag ccggaacagc
    13501 agggtaggga gatctattat ttattggaga tggaaaaaat gaaagaggta gatcaatggt
    13561 tggagcaatt taggaaaata tgggtagcac gtttcgatga actggacgga gtgctcgatc
    13621 gaatgaagaa aaagaacaag gggtaattaa tattcaacaa actcaaataa acaataacat
    13681 ggcaactaaa gtaaacattc acgcaactgt tgctgcaccg cgtgaaaaag tgtgggcatg
    13741 ctacactgaa ccggaacaca tcacaaagtg gaatttcgct gccgacgatt ggcaatgtcc
    13801 ttctgccagt aacgatatgc gtgttggcgg aaaatatact gctcgcatgg aggcgaaaga
    13861 tggtagtttc ggatttgact tcgaagcaat atataatgca attgatccgg gcaattcatt
    13921 tacttatacc attcctgatg gaagggtggt ggatgtagta ttttccgaca atggaagcaa
    13981 tacggatgtt gctatcacct tcgatgcaga atccgtaaat cctgtggaat tgcagcaggc
    14041 aggatggcag tcaatcctga ataattttaa agaatataca gaagggtgat tgcaagatca
    14101 ccttttttta tgagcgttta cgataggata atattgccag tgtattgaaa aatattgcca
    14161 tcgcgccaat agcataaaga tttggtaaaa tatcacgcaa cccgcttccc ttcatgataa
    14221 ccatacgcat cacttcgata aagtaacgaa caggattaaa tcgcgttacc acctgtgccc
    14281 aatgcggcat tccatcaatg gaagtatata atcctcccat cagaataaat accatcataa
    14341 agaaaaacga catcaacata gcttgttgtt gtgtttccgc aagggttgaa atcaataatc
    14401 ctattcccaa caacgctaat aaatacacgg aagcgaataa ataaatcaac cccaaactgc
    14461 cttccggaat tatgccgtac actaatcgcg caatcagcaa accaatagtt aataccacaa
    14521 agcctaatag ccagaaagga attaatttac ctagtatgaa ttgatatttt ttgatcggcg
    14581 atacattcaa ttgctcgata gtgcctactt ctttttcatg cacaatgttc aatgaggcaa
    14641 ggaaacttcc taccatagtc accagaatta ccagaatacc gggaaccatg aacaatgagt
    14701 atttcatatc atcattaaat ctattgatgg tagtaacatc tataattgga acaggatttg
    14761 tttttggaaa tactatccat tcacttctga cctcctgatt gaattgattg ataattgtca
    14821 tcgcatatgc agagccgata tttcccttgg caccatttac agcattcacc gcaagatgca
    14881 attctgattt atcttctctt atcaggtcgc gctcaaaatg atccggaatg gtaataataa
    14941 tatccgcttt atcttgctca attgtttgta atgcctccgt ataattatct acatagccgg
    15001 taatattaaa atgatcactc acctcaaatt tctgcaccaa tcgctgagca taggtgctgt
    15061 gatcattatt tacaatggtg aggttgacat gctttacttc gtaatcggct gcaagtggca
    15121 gtataagcaa ttgaactgcc ggcatgagga aaataatgcg caggatggat ggatttctgc
    15181 gtatctgctg gaattctttc cgcaataaga taagtagtgt tctcatgcca gccttgtttt
    15241 aaatttcttg taactcacaa ataaaaagaa caaagtcatc ccagtgagaa tcaacacttc
    15301 acgccagata ctcgcaaatc ccaggccttt aatcatcaca tcttttacaa tgatgaagaa
    15361 ccattttgtt ggtacgatat tactcaaaac ctgcatgggc acaggcatgt tttcaatcgg
    15421 gaacatgaag ccactcaata tcaatgtagg taacatcaat cccataagtg agataaacat
    15481 tgccgtttgc tgactgtcgg ttatattact gatcatcaat cccaatgcaa tagcggttgt
    15541 tataaacagg aagcatactg caaaaagaag gaaaatattc cctgccacgg gcaatccgag
    15601 aataaatatc gatatcagca aaatggtggt aacattcaca agtgagatag tgatatatgg
    15661 aaccgccttg ctgagtatca atactatcgg ttttaatggt gatgcaagta taatctccat
    15721 tgtattgatt tctttttccc gaacaatggc tattgaagtc atcatcgcgc tgatcaataa
    15781 caatatcata cccatcacac ccggcacgaa taaataagca ccttttaatt gcggattata
    15841 tagcatgcgg acttccggcg taatcgtata tggcagatta taggcaccat tgatatcacc
    15901 ctgaaaatcg cggataacag cacttgcata cacactcaat gttgtagccg tatttggatc
    15961 tgtagcatcg gcgattaatt gaacttgtgc ctcgttgcca tgactaagat cgttttggaa
    16021 attttccggt atgattactg ccaatttaat ttggtctgag agcagggcac tttctacgtc
    16081 ggctacatca tgcacatcgc cgttcaattc aaaatattga ctcgctatga gttgatcacg
    16141 caattgatga gaggcctcat cctgagaaag atcaagtatc gcgatttttg tattttttat
    16201 ttcattggtc agcgcgaaac cgaaaattaa aatctgcgca atgggcaagg caaataatat
    16261 caacagcgat cgcttatcgc ggatgatatg tttaaattcc tttttgataa atactccgta
    16321 ttgtcgcata tcagtcggcg gttcttttag caccacgcgc caattgagta aagatggcat
    16381 caatggtagg tgactgaaat tgttctttca aaatagcagg tttatccatt gcagcaatac
    16441 gaccgtcgac cataatggat atacgatcac aatattctgc ttcgtccata taatgtgtgg
    16501 taacgaacac agtgactcct cgatctgctg cttcataaat gagctcccag aattttcttc
    16561 gagtaatagg atcgacacca cccgttggtt catcaagaaa taccacctga gggtcatgta
    16621 atattgaaat cgagaatgcc agtttttgct tccaacccaa aggcaagtcg cgaacaagcg
    16681 ttttggcact ggatgtcaat tccagtttgt cgagaagcgc tgcactcttc gcttttaatt
    16741 ccttcgcccc aacgccataa atgccaccaa aaaagcgaat gttctcaatc acaggaatat
    16801 cttcatacaa agaaaagcgc tgactcatgt aaccaatatt ttgttttaca tgctctgtct
    16861 gcgtataaat atcgtaacct gcaacagagg cattcccgga ggtaggagag aggataccga
    16921 tgagcatttt catggcagta gtttttcccg cgccattggc tccaagaaaa ccaaatatct
    16981 cgcccttctt tacttcaaaa ctgatatgat caaccgcggt aaaatcacca aaggttttag
    17041 tgagttcact tgcactgatt actatttggc cttccttatg catgcgattc cattaaagcc
    17101 atgaaacaat cttcgatgcc cggggtgatg gggcagaatt ccaataaggt atgtctatcc
    17161 ttcagtgcaa cagttaagcg ctcttgagtc caggcaccgt gtgcaaagcg cacatgcagg
    17221 taatcaccga aaccatagca acctttaatg tcgggcaatt ctcggatatc acgtatcagg
    17281 tgatacatct gttcgcttct tacggcaatc agttcatctg caaaacggtc aactatctgt
    17341 tgcggtgtat ccaattgcat gatctttcct ccctgcataa gcgcaatcct atcgcaaagc
    17401 gtagcctcat ccatataggg tgtactaacg attatggtga tgcgcgactg aaggcgtttc
    17461 aacatctccc agaactcttt ccgtgaaacc ggatctacgc ctgtggttgg ctcatccaga
    17521 aacaatacct cgggtttatg aatcagcgcg cagcataagg caagtttttg cttcatccca
    17581 ccactgagtt tacctgcttt acggctttta aatggctcga gttgcacgta aatatccttt
    17641 accaaatcgt agttttcctc gatgctggtg ttgaacaggg tagcgaagaa gttgaggttt
    17701 tcctctacac taaggtcctg atacaaactg aacttgcccg gcatataccc aattcttcgg
    17761 cgtatctcct tgaaatcttt tactacatct aatccggcca cagttgcatt accgctgtcg
    17821 gcaagccata gggtagtgag gatgcggaaa agggaggtct tgccggcacc atcgggacct
    17881 atcaacccaa aaagctcacc tttgcgcacg tcgaagttga tattgtcgag tgcaacaagc
    17941 ttccctttat taaaggtttt atgcaggtta tgtatgtgga tggcgctttc catgtgtatc
    18001 agaatactac ttcgccatac ataccgatct tgatatttcc ggtattcggt acggtgatct
    18061 tgatggcgta aacaagattc gcacgttcgt ctttcgtcat aatgctttta ggggtgaatt
    18121 cagccttgtc actgatccag ctaacggttc cggcatgttc ggaatattga ccgtcggcac
    18181 tgtctaccaa caccttcacc tgctgaccga tcttgatgcc gggcagttgg tcgccggtga
    18241 cataagcgcg caattgcatt tctgtgaggt cggcgatctt gtaaacaggt tttcccgggg
    18301 cagtgtattc accggtttca gcatattttg tcaatactgt tcctttaatc ggattcagca
    18361 cccttgattg caccaattgg tcatcaatct gggcgatctg cagggtgaga gggtttactt
    18421 cagcatagat actgccggcg gtggtcatca ggcttgtacg ctgcgcatcg agttgcttct
    18481 ccagcaccgc gatctgattc acgatgtcat cgagctgttt agaaggagcg gctcccgctt
    18541 ttacgagaat ttccatgcgt tgtttttcac cggcagcaac ccgaatttgc tcctgaatag
    18601 tagcgatttg tgtaggaatg ttcggttcgc gactcattac agcctgaatt tgtgcattca
    18661 gttgagcctt tttgaggtga agctgggtgg tatcgactat agcagccaca gcattggctt
    18721 cgagcgactg cccctcttca acattgaggg cgagtaactt accggcagtt ccggcgctta
    18781 caatcacttc cgtggcttca aaggtgcctg tggcgtcata tattttgtcg ccatttgaac
    18841 aggctccgag aagcatactt aggcctgcga ttatgatgat tctttgcatg ataggttatt
    18901 ttccgagtgt attcagatag ttcacttttg ttgcgagaag ctgtaggtcg tggatcgatc
    18961 ggttgatacg ggcatcgtcc agatccttgg cataaaggat ataatctttg gttgagataa
    19021 caccctgttc cagttgtaca cgtgccatcg ttagaataga tgtgcgcaga tttaccaggc
    19081 tgtcatcttg tgatatcagg gttttatatt tatctatatc tgactgatat tgagcagatt
    19141 gcacgtttat actctgttca aattgtagct gctgcatgcc gataatctca ctgtttaagc
    19201 caaagatttt cgcatcgcgg cgcttcgtat cgcctgtcca caaagggtaa gaaaccttga
    19261 taccgcccat gtagaaagta ctgaaactat ccaccaaaaa attgagtccc ggcttgccat
    19321 aaccgccctg tgcgaagagg cttactttgg gcaaggtggc tgcttcggca tattgttgtt
    19381 gtagtccggc aagggtgcgt tgctgagcat aaagttgcaa ttcggggcgt tgattgttca
    19441 tagcggtgcc ttcaaaagca ggctcaggtc ttgcgaatac tgaagtcgaa gtgaggggca
    19501 gggcagtgag tacggccatc atacggatgg caccatcacg aagtgctttc agctctgtaa
    19561 gttgttgctc cgttttcatg atctcggctt gcaggatgag aatctgctgg tcgctgacca
    19621 ccccattcgt accgccaacc ttgctgatgg aaaggaccgt attcaaatcg ctcaccgcta
    19681 ttaccatcaa ctcaattctt gcgtcagcca tgagaatcga catgtacacc tgatctacct
    19741 tatcgcgtat cttgaatttg tctacctcta ccttctgcat ttccactgcc aggttctgtc
    19801 cggcgatagc tttttgctgg gaggagatac ctccatcata cagtgtttgg ctgatatcga
    19861 tagtagcctt atactgatct ttgcttaatt ccggtacttc gattccgggg aaagaaatgg
    19921 gcaggctggt tacctcactt tggtaagttg cctgggcatt aacataaact tgtggctgcc
    19981 atattgcatt gattttctgt atattagcat cagttatctc ttgtaatagt gcagattgac
    20041 caagcagtgg ataattggct tcggcagcgc gttggcagtc ggcaagcgtg actgattgct
    20101 gtgcccttag cgttaaaggg agaaggagta tgatcaggaa aaagcgtttc atgcttgttc
    20161 aggtttgagc atttgtaata tgatatcagc cacgactttc ttacgtgact gcatgagtga
    20221 ctcgaactgt tcgggtggca taccactaac ggtgagaaac atgggtttgc cgataaatgg
    20281 gaagatgcaa agggacatca gagacatgag cacatgtaca ggttcgatct gccgaatggt
    20341 gcctttttgc atttcagtcc ggaggctgga tacgaagatc atcatgaatt cacggaccgg
    20401 cattttttcc atttttgatt ttatcatgtc ggggttgcgt gagatctcag tgaggatgaa
    20461 tagaggcaca tcgggattgg ccagcagcat atcgatctcg gcattcacca ggttgcggat
    20521 gcgcatgtcg agtggggcag gcgcattcag gatgctgata aagctgccat agaaatgcga
    20581 catgttctcc tcgaacaccc gttcaaacat tttctcttta ctccggaagt aatagtgcaa
    20641 caaggcgcga ttgatgcctg cctcagtggc tatatcttcc atacgtgctg cagcaaaacc
    20701 cttgcgggtg aatacacgct tcgcagcctg caggatggct tcctctgtat tgcggtcggg
    20761 ggttgtattt tgcgttttac tcattggttt aacaaaattg ttaaacaatg atgcaaatgt
    20821 ataagtaccg ctttcaaatc tccaaattcc gaggaaaata tattttttgc gttatctcaa
    20881 atataatatt atataattaa atataagtaa ttgataatca gatgttaaaa tataaaaaac
    20941 ataacaaaaa ataattgaaa ttcttgcaaa atacgaggcc tgtcaacttg tcaaagtggt
    21001 aaaatgccat taagctgccg gaagattgaa gcggttaaaa tgaggcttat tcaccatcaa
    21061 gaaccacagg agaatacagc gaaggagaaa cgagttctac ccctttttcg tggtagagtg
    21121 cctccagttt cttggtccaa gattcgtcta tcaccaggtt tgaaaatcgg aattcatata
    21181 ggttgccgct gccatacata ctgctgaggt gtgcaccata tcgatctgcc agatccctga
    21241 caaattgatc
//
LOCUS       DAOQOU010000041        20732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1248_length_20732_cov_5.382309, whole genome shotgun sequence.
ACCESSION   DAOQOU010000041 DAOQOU010000000
VERSION     DAOQOU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20732
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1248_length_20732_cov_5.382309"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLM90_05515"
     CDS             complement(<1..360)
                     /locus_tag="PLM90_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HMZ88860.1"
                     /translation="METTIRYDFSLLTEYDIHLFKQGRHFRLFEKLGAHPMSGEGQEG
                     VYFAVWAPNAKGVCVAGTFNHWNTTALPLQCRTDGSGIWEGVVSNVMGGDLYKFIING
                     ANGDVFEKADPFARYAEP"
     gene            497..4372
                     /locus_tag="PLM90_05520"
     CDS             497..4372
                     /locus_tag="PLM90_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ88861.1"
                     /translation="MYRNLRKLALVIALVPCMCMLQAQNSSLMWEITGNGLKEPSYIY
                     GTMHVSDKIAFHLTDSFFIAVKNADVIALESNPEHWIENDFGNPDMSSNYEFDESVGY
                     SGYDGSNMYFGGSDFYTDATEVFIPDQRTYYSAFADNSWLINGYLYRYADQRGDYEEE
                     TYLDLFIFQCGKKLSKQIAPLEREQEVLRLLEKANAEPDKDQKEAENAEFLEQERMRA
                     KLEEDGKDIYELIDEAYRAGNLDKLDSLERVVSPSRHYHYYFIEERNRNMMRRMDSIL
                     QTGKSMFTGIGAAHLPGQNGAIEILRSMGYTVRPVMGKVNNKSIKEKEKIDGIYFAQP
                     TKFQYAYDSLFTIESPGKLYEIGGYQTNSFFMYPDMANSAYYAILRVAHHAYLRNKTP
                     QDVYNEIDEMLYENIPGDIQSKTKITTNNGWPGFDIISKTKRGNYLRFKIVVSPVETI
                     IFKASGIGDFMIKAKEPETFFSSIKFRNTNSNAWSNYTPEWGGLSVSLPDIRVTDDVP
                     DGASVSNAEIVSIGEGVAGENKYWMSTYYLNDIKYIEEDSFELVRIADVFAKQFKKQD
                     YKNQGYKFSTYQGKPACTGVWKSAQGTYLHTQSVINGPYYIFMTAFTNNNTVPSDYFN
                     SLKLIDLTYNQEFTTYTDTSLYFTVKTIFPEPDTLLTLEEVDMGGYNDYGYNDDDDED
                     EIDEDYLELTRKTYFSSETTPENIYVVYHKYNDYTYFKNKKAFWNSFDTVYSSSFNLT
                     RRKFSEKDDIQSLEFLLTDTNSTRGIYKKFILKDNAIFQLSTCIDTTKAPSLFVSNFY
                     NTFTPSDSVDGRSVFASPADNFFAALDGTDTMKRKQAIAAVDIVNLDNSDAPRMISYM
                     ESSKFKKMKMDDRVEFINALSTLKHATVVPELKKIYNDAGDSSSLQTAVLNALSEMHT
                     LEATKAFSELLYIETPLAQKGYSLGWIFSDFSDSLKLAASLFPQLWDFTSFPEYHYPT
                     YRLLAKLYAEDSTRLKLYADHKKQVITQASAELKRNLTDDDEDDDDNSYSRYYNKHYF
                     TKLKDITDNDEKAVGNFYGTDQFAEKDEDYIGLSTNLISRLGLEDDDLNTRFDWASSL
                     QDYYSILLTPFYDDPMVKKYYDRILRCKDKDLILSTALTMLEKGLPVPDSVWTNLQKS
                     REYRYETIDALMEMGRKDKIDTTYGNQRMITECILYQYDFTKDEDSIQFITKTLVHTR
                     EGDGYIYFYKSKMGKDKGWSLDCTGLQPENGATYTIEPMFVHYGDVILDEEDQQKKMD
                     EIIRNIELLGRDRVSIKFDGDKSYDYYDY"
     gene            4372..5226
                     /locus_tag="PLM90_05525"
     CDS             4372..5226
                     /locus_tag="PLM90_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ88862.1"
                     /translation="MRYIALLFLLTCTINNQIIAQVDSSHANNQFTASPFTFNLNLKT
                     DLIISGTAGLSWGGTLFTTHQPLNITSLAALDTNNLIPLDRYPFSVDSAEAADWVIIS
                     DVANLASFGAPLVVGLLLLSEKNEALTYAVMYAEGFTLTYGVTQLLKEAVSRPRPYAY
                     QDNYPIEYRLSKDEWASFPSAHTSSAAFNCFFAAYMLDKMLLDKQADEKLRIAIWAAA
                     VALPAYTGFARVSAARHFPTDVAAGYVLGAGLGLLIPYLHEHKAQGIAMTPLFSNEYL
                     GASIVIQR"
     gene            5301..5726
                     /locus_tag="PLM90_05530"
     CDS             5301..5726
                     /locus_tag="PLM90_05530"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ88863.1"
                     /translation="MPAKKTKLVEIKTKATIASVDDFVNAISDEQTRNDCHAIIKMME
                     KASGDPAVMWGPAIIGFGHRIYTSEKTGRSVDWMKIGFSPRKANISLYLSVDINKHTD
                     TLKKLGKHKTGKGCLYINKLADIDVKVLEGLIKESLKNK"
     gene            5763..6416
                     /locus_tag="PLM90_05535"
     CDS             5763..6416
                     /locus_tag="PLM90_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88864.1"
                     /translation="MKKQYFIAIAVTFLLATLGMGLWLAEILFVKGWHGLQWLSGILY
                     TPYFITMIAAIAFILPFAFVNRRSIKQLLIPALLIYFTNLVFYVIGTYLCYAVYYRFL
                     IFNIRYLQNIYFDIILYFILFGFIYWLVIDKLINKVKFVKMLLISFAAALSVILSLAT
                     VAIIPGFGTGTDLIDAVKMGYNVFWIIFMLGISGWSIAVKKPMAQKLNQDANQIDLV"
     gene            6726..11966
                     /locus_tag="PLM90_05540"
     CDS             6726..11966
                     /locus_tag="PLM90_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ88865.1"
                     /translation="MKRSFIIVGNKVQDKPDLVTLDGYTTSHAIDLTGSRDGGITHEI
                     DADEDDIVEFEFQDDSFWMGTVGEIPLLIQNEASPTARDYSGPLEIPQVLSMGSDQRG
                     FLRNVILKRLHIRKPAEIITSIIVKKSAQLLDAHIQESPGLYFVDKNFRTKIINQKLT
                     EINQPYLLMIHGTAASLDGSFDKMIKNNQFGLWDSIQKQYGDRILALQHYTLSKSPVE
                     NAIEAIKWMPKNATLHIMSTSRGGLVGELIAKLSDKGNPSFKPENRQLIADAFGDANV
                     KRLEDEISSRNITVEKFIRTACPAAGTSLCSKRLDRFLTVLINGIQFATVGASAPALA
                     PFKKLVADILACKAKPDVLPGLYAMNPDSGFIKALNHPQESIGSPLYVIAGNNKISLK
                     PKAFVTILSKLIFYGKNDWIVDTKSMTRGIFRKQNIGYFLDEGDDVNHFHYFANRDTQ
                     EAIKEAVNAPTNAIASKFESFDRNLASNADRGWVFGNLEPKPVSGEKPVIVMLPGILG
                     SNLSKDGHEIYLSIDDIMAGKMTQLNMQATNVKATSLIKKFYGKFAKEFDNDYDVAVF
                     PYDWRLDLRKEADRLNDYITNILHRTGNHSIKIVAHSMGGLVVRSFIQRHANTWNAIK
                     QRSDYRVIFLGSPLGGSYLIPEIMVGSGGRIKQMANMDLIHSKNTLLEVFNKYDGLFN
                     LLPADTSIHDFTSLQTWQEMKQNFTSFNWPIPSSATVNNYKSFHTEVGNFDRSVYLDK
                     NIIYIAGKSDGTTNGFYYKDHAVDGDKLTFTETPYGDGSVTWESGIPAEIKSGDRVYY
                     VRVGHGDLANETSMFPGIKELLIAGKTSKLSKNPPQQRGEIPVSDKRDELIQDVTPEE
                     FENTMIGSTPTEPVDEEFTTPINVVLRCGDLKYASYPVLVGHLKGDGIMSAESVVDHY
                     MSNMLTKRLATGNYPGDIGESKYFKNNPGMPKGTLIVGMGKTEKLSGFQLEETVKQGV
                     IEYLCEFRVNEGNVHGTVGLSSLLLGCGYAGLSVETAVKAVISGVHKANQSVRQNGDN
                     IWPTVDEIEFIEIYEDRALQALICLDNLKHTTNAGFNFEPTLYNLLGSRKRLLFEQNA
                     DWWQRVTIREQIDCEQPSKGKIFTFTSSMGTARDEMRKLYCTKEVIDSLMKEISSRDV
                     WDKELAKTLFELMIPNDFKLALRNQQNLLLIVDETAAAYPWEMLQDISIHSQPLATTA
                     GMIRQLTTEDFRPSVNYTLDNTALVIGNPNTANFLPALQGASDEAELVNNVLMDAGYQ
                     STLSNNESSSVIIRKLFRSDYKIIHLAGHGVYNHAQPDLSGMVIGNNVFLTTREIQQL
                     SFIPELVFINCCHLGNIEAMDEALYQNRYKLAANLGTQLINMGVKAVIAAGWAINDNA
                     AKDFTEKFYAAMLSGSNFGDAVRIARHYCYEHYPYSNTWGAYQCYGDQFFTLKRLNSG
                     KKPSLRKYYVANQVRIDLENLINRIGANYSEIQKAAYLDEINLILDSAAAANIPQSSL
                     YECLGIIYNRLGNDSAAIENFKLYFEKSSETFNVSSYVEYARLLLSHYTHDKLAAKKI
                     RVVVAELENLSKTIPSYAIFNAIGTGYKILARMSKGADHSKNMLHAFRAYEHAYNSGK
                     QAGVDNTMYALSNAFMLGIALQDKKLQLPDISELEWSFNNIKAFDNTLTFWDLMDEPR
                     YWLCKFVMQHQGFVFPHGNVKVKAETVIDAWEKVVKTGGMETQQNEMIAKFASMVTFF
                     DPKKFSKLRPKLDQMMDALK"
     gene            12090..12659
                     /locus_tag="PLM90_05545"
     CDS             12090..12659
                     /locus_tag="PLM90_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMZ88866.1"
                     /translation="MRNLVFFMHASLDGFVGGPQGQLDWISVNDEIFDFVATMTDAAD
                     TALYGRVTYNMMESYWPTAGDKPNASKHDKEHAVWYNAVSKIVLSTTITAEGRKNTTV
                     ISDNITDAIQEIKAQEGKNILIFGSPSASHTLLNLGLIDEIWVFVYPVIIGSGIPLFK
                     DIQKKTALELVESKVFSNGGIALHYRIRK"
     gene            12770..13408
                     /locus_tag="PLM90_05550"
     CDS             12770..13408
                     /locus_tag="PLM90_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88867.1"
                     /translation="MSESELPEHKTGGFKILEIVPEIIAAFQIIISPLLIGSIIGAVI
                     YLTEPSPTRLLIGILIGILGLVIGIIWAVRQARHKGAAWFMSRTIASPELDEKPYDFK
                     ISYRFLSAEEGGRKTGPPFQGYRPDFVFAVDANENVKSPVAWMIHPIFADADGQVIEN
                     KTTAVAEEGIAYMRVIFREHKHLYIDKIKVGEKGYMIEGPYKVALCSIIEVY"
     gene            13439..15280
                     /locus_tag="PLM90_05555"
     CDS             13439..15280
                     /locus_tag="PLM90_05555"
                     /inference="COORDINATES: protein motif:HMM:NF003780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HMZ88868.1"
                     /translation="MKPNLIQASICTCLIMLGLSMHGQTTSKGPQPIITNGEAQIVPE
                     YRDRLVQELWVETTFDSDGDGRLDRMHTYVTRPTVTDSFDLKLPVIYTTSPYYGLRLW
                     ALLDLFRGNTYWNVKHELGEQPKPHHHSNLGTRTKRPFMAFYMDNLWTTRGFIMVYSS
                     SPGTGLSDGSPTVGGENESLAPKAVIDWLCGRGKAYSTRTGNDEVKAFWCNGKVGMTG
                     TSYDGTLCIAAATTGVQGLEAIIPVAPVTSFYNYYRSNGLVRSPDGYLGEDMDVLYDF
                     INSGDKSKRARNDREVRDSILVKNIDRVTGDYNAFWASRDYLNDIDNMHCAMLMAHGF
                     NDWNVMTEQSYRFYLAAKEKGLPVQLYYHQQDHGGDPPLTMMNRWFTHYLFGENNGVE
                     NDPPVQIVREFEELPTPYAAFPDPDAENVTLYLQGGVNNTGSLIIAANNTLVLDTLVD
                     DYTISGDMLIQEKYSEHRLLFVTPTLKKDIRISGIPSVNIRMASSAQAANLSVWLVAL
                     PWEDEKNTQLYENIINRSWADPQNYRSISKGELLKVGEFYQVNFNLMPDDQVIPQGQQ
                     IGLMIFSSDKDFTIAPKPGTELTIDLSRTNITIPFVGGVEAYEDAVK"
     gene            15292..16035
                     /gene="arr"
                     /locus_tag="PLM90_05560"
     CDS             15292..16035
                     /gene="arr"
                     /locus_tag="PLM90_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HMZ88869.1"
                     /translation="MEFSPQNAIVRLCLAGMEQETNGDPLAAKTLFMEAWSTSTNDFE
                     KFISAYYIARHQTSPADNLHWLQLAMEFALSANTLSASSALPNVYAKIASCYKEIGDD
                     AQSSKYRQLSEESAHQLPDKGPFYHGTRADLQTGDDLLPGNRSNYQSDIIMNHIYFTA
                     LLNGAGLAAALAKGEGRERVYIVEPTGDFENDPNVTDKKFPGNPTRSYRTTFPLRIIG
                     EVMEWERQTPEQIQQWKDRLAKNSGEIIN"
     gene            16057..16725
                     /locus_tag="PLM90_05565"
     CDS             16057..16725
                     /locus_tag="PLM90_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HMZ88870.1"
                     /translation="MYHFSYFKEKDRATIIDFMHKHPFAFVTGSFHDGRQVATQVPLL
                     IEERNGELVLQGHFMRNTDHHKAFIENPNALIVFTGPSAYVSASWYSNPQIGSTWNYM
                     SVHVTGQMTFMSHEQLIQLMKKLTLHFEKENAASPTIYDNLPDTFLDKMMPAIVGFEV
                     KAQKLENVFKLSQNRDPQSYDNIIMQLEQLGGESMLIAQEMKNRRGELFAEGSEWDPN
                     KFAS"
     gene            16729..17169
                     /locus_tag="PLM90_05570"
     CDS             16729..17169
                     /locus_tag="PLM90_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ88871.1"
                     /translation="MTNEHEIVSSRIISASIDKLYQAFADPEKLAQWWGPKGFTNTIH
                     AFDLRVGGKFELTMHGPDKGNYENASVFTKVIPLNLISWHRITQPWFDMEVIFQDIDG
                     NSSSITFRMIFDSAESCQKLRRFVEPNNEENFDRLEAMLATSTL"
     gene            17874..19340
                     /locus_tag="PLM90_05575"
     CDS             17874..19340
                     /locus_tag="PLM90_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88872.1"
                     /translation="MTFLLITAGFKCFCQDWALFPHGTDMYYFEEGTTTLFSCKQDSI
                     LVAGDTTIYYFNKIAPEGATQACYDSILDYDEYTMFSWPSIKMEFTGVDYNLFFYCDE
                     FGALPAIFKSLAAVGSNWEIPNASDYSEFSTVTITCDSIILGSFLGITDSLKYYSIQT
                     VAPLTGTYTIDKAQYILSKHNGLIRFLPFQKLLNPTTCEDCFKCYDLVGQHSDIGTIG
                     WSGPLWENWIQLSEGDFLKYKHYSNSIFGTTESYTTALIQSVQHFSDSIVIESLNADG
                     LLGTSTYYKAAIFNAINAPVWQPFYIPEYHNQPELDRLIGIADSYETIVDTTTFPGHW
                     RYKQSLVTDAVQDNDFCYVEPSFEYGHEIRWDSYIGWTFESSGGPKYYNYSLLIGYVI
                     DGQTYGNYWPLTAEELAMQKPLLSIFPNPATNTITVNLPLETFSQYEICDITGKICSS
                     GTMQNMSQTIDIGDIPSGTYILKVASDHAIQTGKFVKL"
     gene            19459..19857
                     /locus_tag="PLM90_05580"
     CDS             19459..19857
                     /locus_tag="PLM90_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88873.1"
                     /translation="MIIRMQTSATTPLLILVAGPYRSGTNDDPALIAANVKAMTDTAL
                     ELYRLGHMPVLGEWFALPLIEAAGSKQIGDAIFNELFHPVAVKLIDHCDAVLRIGGPS
                     AGADEMVNTGKAKGKLIFMEMKEIPQLNNK"
     gene            complement(19863..20234)
                     /locus_tag="PLM90_05585"
     CDS             complement(19863..20234)
                     /locus_tag="PLM90_05585"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HMZ88874.1"
                     /translation="MMQTFRCLPAMLLIVGITACSSNTRPIDETHASKSSPTATSSTQ
                     YTCIPFQVEGGWGYDILIDTVRYVHQEHIPAINGLHPFASESDAYKSGELAIKKLEQG
                     IMPPTITVEELRELGIVITAL"
     gene            complement(20231..>20732)
                     /locus_tag="PLM90_05590"
     CDS             complement(20231..>20732)
                     /locus_tag="PLM90_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88875.1"
                     /translation="VNRDDFWEYDPIADTWTQMPDFPGGARVNATAFVIDTLAFVGMG
                     YDTSFNYDIWLWGDTTNIVPVDTQDAVTDLHALQATVQLFPNPVTAYANIDIQQMQDG
                     DDLIIEIFDLNGSNVTRDCIVSWSGITSDHYTGKMDVHALPAGTYQCVVHNGKAHKAT
                     KFVVIK"
BASE COUNT         6519 a         4095 c         4245 g         5873 t
ORIGIN      
        1 tggttcggca tagcgagcga aggggtctgc cttctcaaaa acatcgccat tggctccgtt
       61 gatgataaat ttatacagat cgccacccat tacgttactg acaacacctt cccaaattcc
      121 tgagccgtcg gtgcgacatt gcaatggtaa tgctgtggtg ttccagtgat taaaagtgcc
      181 tgcaacgcaa acaccttttg catttggcgc ccatacagca aagtaaaccc cctcctgccc
      241 ttctcccgac ataggatgag cacccaactt ttcgaacaat ctgaaatgac gtccttgttt
      301 gaacagatgg atgtcatatt cggtaaggag ggagaaatcg taacgaatgg tggtttccat
      361 gctgcaaagg taggatgcat ccgttatttt ggaaaaacag gtggatgagt tgtgtaaaaa
      421 taggttgtga ttatacgata tcttatggat ttgatgtttt attagtatca ttgctttaca
      481 ctccttaaac caaactatgt acagaaacct cagaaaactt gcactcgtta ttgcacttgt
      541 gccatgtatg tgtatgcttc aggcacaaaa ttcatcgctc atgtgggaaa tcaccggaaa
      601 tggactgaaa gaaccatcct acatctacgg cacgatgcat gtgagcgaca aaattgcatt
      661 tcaccttacc gacagttttt ttattgccgt taaaaatgcg gatgttattg cattggaaag
      721 caatccggaa cattggattg aaaacgactt tggcaatccg gacatgtcgt cgaactatga
      781 atttgacgaa tcagtaggat atagcggata cgatgggagt aatatgtatt tcgggggaag
      841 tgatttttat accgatgcaa ctgaagtatt tattcctgat cagcgcacat attattctgc
      901 ctttgccgat aacagctggc tgataaatgg ttatctctat cgctatgccg atcagcgtgg
      961 tgattatgag gaggaaacct atctggacct gttcattttt cagtgcggta aaaagttatc
     1021 aaaacaaatt gcaccattgg agcgcgagca ggaagtatta cgattattag aaaaagcaaa
     1081 tgcagaacct gacaaagatc agaaggaggc agaaaatgct gaattcctgg agcaggaacg
     1141 catgcgtgct aagttggagg aagatggaaa agacatttat gagttgattg atgaggcgta
     1201 tcgcgcagga aatctcgata aattggattc actggaacgc gttgtatccc cttcgcgcca
     1261 ttatcattac tattttattg aggaacgcaa cagaaatatg atgcgcagaa tggattccat
     1321 tttgcagaca ggcaaatcga tgttcactgg cattggtgcg gctcacctgc ccggtcagaa
     1381 tggagcgatt gaaattttgc gcagcatggg atatactgtt cgtccggtaa tgggtaaagt
     1441 gaacaataaa agcattaagg aaaaagagaa aattgatggc atatattttg cccaacctac
     1501 aaaatttcaa tatgcctatg actcactatt caccattgaa tctccgggaa aattgtatga
     1561 aatagggggc tatcagacca attcattttt catgtatccg gatatggcga acagcgcata
     1621 ctacgccatt ttgcgcgttg cccatcacgc ctatcttcgc aataaaacac ctcaggatgt
     1681 atataatgaa attgatgaga tgttatatga aaatattccg ggtgatattc aaagtaaaac
     1741 aaaaatcact accaataatg gttggcccgg atttgatata attagtaaaa ccaagcgcgg
     1801 aaattatctg cggtttaaaa tagtggtaag ccctgttgaa accatcattt tcaaagcgag
     1861 tggtattgga gatttcatga tcaaggctaa agaaccggaa acatttttct cgtctataaa
     1921 atttcgaaat acaaacagca acgcctggtc aaattacaca cccgaatggg gaggattaag
     1981 tgtgtcgctg ccggatatac gtgttacaga cgatgttcct gatggcgcaa gcgtcagcaa
     2041 tgcagaaatt gtttccatag gtgaaggcgt ggccggtgaa aataaatact ggatgtcgac
     2101 ttactatctc aacgacatca aatatataga agaagacagc tttgagcttg tgcgtattgc
     2161 agatgttttc gcgaaacaat tcaaaaagca ggattacaaa aatcaggggt ataaattcag
     2221 cacttatcaa ggtaagccgg cttgtaccgg cgtatggaaa agcgcacaag gcacctattt
     2281 acacacgcag tcggtaataa atggccccta ctatattttc atgacggcat tcaccaataa
     2341 caatactgtg ccaagtgatt attttaattc attaaaactc attgacctca cctataatca
     2401 ggaatttaca acttatacag atactagttt gtatttcact gtgaaaacaa ttttccctga
     2461 gcccgacact ttgcttacac tggaggaagt tgatatggga ggctataatg actacggcta
     2521 taatgatgat gacgatgagg atgaaattga tgaagattat cttgaattaa caagaaaaac
     2581 ctatttctca tcagaaacaa cacctgaaaa catctatgtt gtttatcata aatataatga
     2641 ttacacgtat ttcaaaaata aaaaagcgtt ttggaattca ttcgatacag tttactcgtc
     2701 atcattcaat ttaaccagac gaaaattttc agagaaagac gatatccaat cacttgaatt
     2761 tttacttacc gacacgaaca gcacaagagg tatttataag aaattcattc ttaaagacaa
     2821 tgccatcttc caattgagca cttgcattga tactacaaag gcgccttctt tatttgtcag
     2881 caatttctat aacaccttca ccccttctga cagcgttgat ggtcgcagtg tgtttgccag
     2941 tccggctgat aatttctttg cggcactgga tggcactgat acgatgaaac gcaagcaggc
     3001 aatagctgct gttgatattg tgaatctcga caacagcgat gccccacgca tgataagtta
     3061 tatggaatca tcaaaattca aaaaaatgaa aatggatgat cgcgttgaat ttattaatgc
     3121 tttatccacc ttgaaacatg ctactgttgt tccggaatta aagaaaattt ataacgatgc
     3181 cggtgatagt tcttcattac agaccgccgt actgaatgcc ctttcggaga tgcataccct
     3241 ggaggccact aaggcattta gtgaattgtt atatatcgaa accccattgg cacaaaaagg
     3301 atattctctt ggatggatat tctcggattt ttctgattca ttgaaactgg cagctagcct
     3361 gttcccgcaa ttatgggatt tcaccagttt cccggaatat cattacccca cttatcgatt
     3421 gttggcaaaa ttatatgctg aagacagcac gcgtcttaaa ttgtatgccg atcacaaaaa
     3481 gcaagtgatc acacaggcgt ctgcagaatt aaagcgcaac ctgacggatg atgatgaaga
     3541 tgatgatgac aatagttact caaggtatta taacaagcat tacttcacca agctgaaaga
     3601 catcaccgat aacgatgaaa aagcagtagg caatttttac ggcaccgatc agtttgccga
     3661 aaaagatgaa gattatattg gcttgagcac caatcttatc agtcgccttg ggttagaaga
     3721 tgatgacctc aacacgcgat tcgactgggc aagttcttta caagattatt attccatatt
     3781 gcttactcca ttctatgatg atccgatggt gaaaaagtat tatgatcgca tattgcgttg
     3841 taaggataaa gatttaattc tcagcactgc acttaccatg ttggaaaaag gattaccggt
     3901 tcctgatagt gtttggacca atttgcaaaa gagcagagaa tatcgctatg aaactattga
     3961 tgctttaatg gagatgggcc gcaaggataa aattgatact acttacggca accagcgcat
     4021 gataacggaa tgtatactgt atcaatatga tttcacgaaa gacgaggaca gtattcaatt
     4081 catcactaaa acattggttc acacacgaga aggcgacggg tatatttatt tctataagag
     4141 caagatggga aaagacaaag gctggagcct ggattgcacc ggattacagc ctgaaaatgg
     4201 cgccacatac accattgagc ccatgtttgt gcattatggc gatgtgattc tggatgaaga
     4261 agatcagcaa aagaaaatgg atgagatcat tcgcaatatt gaattattag gtcgcgatcg
     4321 ggtgagtatc aaattcgatg gcgataagag ttatgattat tacgactatt aatgcgatac
     4381 attgcattat tatttctatt gacctgtaca attaacaatc aaataattgc acaggttgat
     4441 agttcgcatg caaataatca atttactgca tcaccattta catttaattt aaaccttaaa
     4501 accgacctga tcatttcagg cactgcggga ttaagttggg gaggaacgct tttcaccact
     4561 caccaacctt tgaatattac ttctttagcc gccttagata ccaacaattt aattccatta
     4621 gatcgatacc cattttctgt agattctgca gaagccgcag attgggttat tataagcgat
     4681 gttgcaaatc tcgcatcctt tggtgcacct cttgtagttg gattattgtt actatcagaa
     4741 aaaaacgaag ctttgaccta tgccgtcatg tatgcggaag gatttacatt gacatatggt
     4801 gtaacacaat tattgaaaga agctgtaagc agaccgcggc cttacgccta ccaggataat
     4861 taccctattg aatacagact gagtaaagac gaatgggcct cttttccaag tgcacataca
     4921 tcttctgctg cctttaactg ttttttcgca gcatatatgt tggataaaat gttactcgac
     4981 aaacaagcag atgaaaaatt gcgcattgct atttgggcag cagcagtcgc tctgcctgcc
     5041 tatacaggat ttgctcgtgt aagcgctgcc aggcattttc caacagatgt tgcggcaggg
     5101 tatgttttgg gagccggact aggcttgtta ataccgtatc tgcatgaaca caaggctcaa
     5161 ggaattgcaa tgactccact tttcagtaat gaatatttgg gcgcaagcat tgttatacag
     5221 cgctaaaaaa gcattcgagc atttaaactt attgggattt cggatttgat ttatgtacat
     5281 ttgaataaaa taatatccgg atgcctgcaa aaaaaaccaa gcttgtagaa ataaaaacaa
     5341 aagccactat agccagtgta gatgattttg tgaatgccat cagtgacgaa caaacacgca
     5401 acgactgcca cgctatcatc aagatgatgg aaaaggcaag tggcgacccg gctgtaatgt
     5461 ggggaccggc aatcatcgga tttggacaca gaatatacac cagcgaaaaa actggtcgct
     5521 cagtagattg gatgaagatc gggttttcgc ccaggaaggc aaatatttct ttgtatttga
     5581 gtgttgacat caacaaacat acagacaccc taaaaaaact gggtaagcat aaaaccggca
     5641 aaggatgttt atatatcaat aaattagctg atattgatgt aaaggtattg gaggggttga
     5701 ttaaagaatc attgaaaaat aagtaataag gattttgttc ttgatttttc atcaacccta
     5761 aattgaaaaa acagtatttc atagcaatcg cagtaacttt tcttcttgct actttgggga
     5821 tgggcctctg gctagcggaa atcctatttg tcaaaggctg gcatggcttg caatggcttt
     5881 caggtatttt gtacaccccg tattttatca ctatgattgc cgcaatcgcc tttattcttc
     5941 cttttgcatt tgttaatcgt cgttctatta aacaattact gattcccgca ctactaatct
     6001 attttaccaa cttagttttt tatgttattg gcacatactt gtgttatgcc gtgtattaca
     6061 ggtttctcat ttttaatatc agatatctcc agaatatata ttttgacatc atactttatt
     6121 tcatcctatt tggatttatc tattggctgg taatcgacaa actgatcaat aaagtcaaat
     6181 ttgttaaaat gttactgatc agctttgccg ctgcactctc agtaatacta agccttgcaa
     6241 ctgttgcaat aattcccgga tttggaactg gcactgacct gatagatgct gtaaaaatgg
     6301 gatataacgt gttttggatc atctttatgc tcggaatatc aggttggtca attgcagtca
     6361 agaaacctat ggcacaaaag ttgaatcaag acgctaatca aattgatttg gtttaatcac
     6421 ctgtttaatc aaatagctca gtctgatacc gcacttttca acctttctgt gacatattcc
     6481 aacacaagag tatactagct ttcagccaac acctatgtca caaaatcatg caaaatttgc
     6541 atcaaaatga gacatttttg acattaacgg ttttccggta atttgcgaaa aatccttgca
     6601 aataaaggcg ctatatacta ccttggatag gtttaccgcg attgaacttg aaatactccg
     6661 tcgcgatgaa tatgtaagta ttcattttta acaaatcgat acaatcttct ataaacatat
     6721 attttatgaa acgttcattt atcatagtag gtaataaggt gcaggataaa cccgacttgg
     6781 ttactttaga cggatatacc acttcgcatg ccattgatct gactggaagt cgggatggag
     6841 gcattacaca tgaaattgat gccgatgaag acgacattgt tgaatttgaa tttcaggatg
     6901 atagtttctg gatgggaact gttggtgaaa tacctttatt aattcaaaat gaggcatccc
     6961 ctactgccag agattattcc ggtcccttgg aaattcctca ggtgctctct atggggagtg
     7021 atcagcgtgg gtttctacgc aatgttattt taaaacgttt gcacattcgc aaaccggctg
     7081 agattattac gagtattatc gtgaaaaaat cggcacaatt gctggatgcc catattcagg
     7141 aatcaccggg attatatttt gtcgacaaaa acttcagaac gaaaatcatt aaccaaaagc
     7201 tgacagagat aaatcagcca tatttactta tgatccatgg aaccgctgct tcactagacg
     7261 gttcatttga taaaatgatc aaaaacaatc aatttggttt atgggatagt attcaaaaac
     7321 aatatggcga tcgcatcctt gcacttcaac attacacact ttctaaatca ccggtagaaa
     7381 atgcaatcga agctatcaaa tggatgccaa aaaatgctac gctgcatatt atgtcaacca
     7441 gtcgcggcgg acttgttggc gaactcatcg cgaaattgag tgataaagga aatccatcct
     7501 tcaaaccgga aaacagacaa ttaatagctg atgcattcgg tgatgcaaat gtaaaacgtt
     7561 tggaagatga gattagcagc agaaatatta cggtagaaaa gttcatccgc accgcatgcc
     7621 ctgctgccgg aacaagctta tgttctaagc gcctcgacag atttttaacc gtattgatca
     7681 atggcataca attcgctaca gtaggcgcct ctgctcctgc cctcgcgcca tttaaaaaat
     7741 tggttgccga tattttagct tgcaaggcaa aacctgatgt gcttccggga ttatatgcca
     7801 tgaatccgga ttcaggattt attaaagcat taaatcatcc tcaggaatca atcggatcgc
     7861 cactatatgt tatagcaggt aacaacaaaa tatcgcttaa accaaaagca ttcgttacca
     7921 tactgtccaa actgatattt tacggtaaaa atgattggat cgttgacaca aagagtatga
     7981 ctcgcggaat tttcaggaaa caaaatattg gttacttttt ggatgaaggt gatgacgtaa
     8041 atcatttcca ttactttgca aatcgcgata cacaagaggc tattaaggag gctgtaaatg
     8101 caccgacaaa tgccatcgcc tcaaaatttg aatcctttga tcgcaatctt gcttccaatg
     8161 ccgatcgcgg atgggtattc ggcaacctgg aaccgaagcc tgtcagtggt gagaaacctg
     8221 tcatcgtaat gcttccggga attttaggaa gcaatctgtc taaggatggt cacgagatat
     8281 acctcagcat tgatgatatc atggcgggta aaatgacaca acttaacatg caggctacaa
     8341 atgtcaaagc cacctcattg atcaaaaaat tctatggaaa attcgcaaaa gaatttgata
     8401 atgattacga tgtggcagtt tttccatacg attggcgttt agatttgcgc aaggaagccg
     8461 acagattaaa tgattacatc accaatatcc ttcatcgcac aggaaatcat agtataaaaa
     8521 ttgtggcaca cagcatgggc ggacttgtgg tgcgttcatt tattcaacga catgcaaata
     8581 cgtggaatgc cattaaacaa aggtctgact acagagtgat attcttaggc tcaccactcg
     8641 gaggctccta tctcattcct gaaattatgg ttggctcggg aggtcggatt aagcaaatgg
     8701 caaatatgga cctgatacat tctaaaaata ctttattaga agtatttaac aagtatgatg
     8761 gtctgttcaa tttgttaccg gcagatacaa gcatacatga ttttacgtcg ctgcaaacat
     8821 ggcaggagat gaaacaaaat ttcacttcct ttaattggcc aataccatca tcagctactg
     8881 taaataatta caaaagcttt catacagagg ttggcaactt tgacagatca gtttatctcg
     8941 ataaaaacat tatttatatc gccggtaaaa gcgatggtac tacgaatggt ttttactata
     9001 aagaccatgc agtagatggt gataaactta catttacaga aacaccgtat ggcgatggca
     9061 gtgtgacctg ggaaagcggg attcccgctg aaataaaatc cggtgaccgt gtttattatg
     9121 tacgcgtcgg tcacggagat cttgccaatg aaacgagtat gtttccgggg attaaggaat
     9181 tgttgattgc aggtaaaaca tccaaactca gcaaaaaccc accacaacag cgaggagaaa
     9241 ttcccgtttc cgataaacgc gatgaactta ttcaggatgt aacacctgaa gaatttgaaa
     9301 acaccatgat aggttccaca ccaaccgaac ctgtggatga agaatttact acaccaataa
     9361 atgtagtgct aagatgcggt gatttaaaat atgcatccta tcctgtcctg gttggtcacc
     9421 tcaaaggtga tggcattatg agtgctgaat cagtagtaga tcattatatg tcgaatatgc
     9481 ttacaaaacg attggcaaca gggaattatc ccggcgatat aggtgagagt aaatatttta
     9541 agaacaatcc cggtatgccg aaaggcacac tgatcgttgg catgggcaaa acagaaaaac
     9601 tatccggatt tcagcttgaa gaaacagtaa aacaaggggt gatagaatac ctctgtgaat
     9661 tcagggtaaa tgaaggtaat gtgcacggaa cagttgggtt gagttctttg ctgttaggtt
     9721 gcggttacgc agggctgagt gtagaaacag cagtaaaagc agtcatctcc ggcgtgcaca
     9781 aggcaaacca aagcgtgcgg caaaatggcg ataacatctg gccaacagtt gatgaaattg
     9841 aattcattga gatctacgaa gacagagcgc tgcaagcatt gatttgtttg gataatttaa
     9901 agcatacaac aaacgcaggt tttaatttcg aaccaacctt atataattta ttgggcagtc
     9961 gcaaacgatt gttgtttgaa caaaatgctg actggtggca gcgagttacc attcgcgaac
    10021 aaattgattg cgaacaacca tccaaaggga aaatatttac tttcacttca tccatgggca
    10081 ctgcccgaga tgaaatgcgc aaattgtatt gcacgaaaga agtaatagac tctttgatga
    10141 aggaaatatc aagtcgcgat gtgtgggaca aggagcttgc aaaaacactg ttcgaattga
    10201 tgattcccaa cgattttaaa ctcgcactgc gcaatcaaca aaacttgtta ttgatagttg
    10261 atgaaactgc tgcagcttat ccatgggaaa tgttgcagga catatcaatt cattcacagc
    10321 ctttggcaac aaccgcagga atgataaggc aattgacaac agaagatttc agaccttctg
    10381 tcaattacac cttagataac acggcgctgg ttattggaaa tccgaacact gctaatttcc
    10441 tgccggcact tcaaggcgca tctgatgaag ccgaattggt aaacaatgtg ttgatggatg
    10501 ccggttatca atctaccttg agtaataatg aatcatcaag cgttattatc aggaaattat
    10561 tccgttccga ttacaagatc attcaccttg ccggtcacgg agtatacaat catgcacaac
    10621 cggatctttc gggtatggta atagggaaca atgtattctt gaccacacgt gagattcaac
    10681 aactaagttt cattcctgaa ttggtcttta tcaattgctg tcatttagga aatatagaag
    10741 caatggatga agcattatat caaaacaggt acaaactggc agctaattta ggcacgcaac
    10801 tcattaatat gggcgtaaaa gccgttattg cggcaggatg ggcaattaat gataatgcag
    10861 cgaaagattt cacggaaaaa ttctatgccg caatgctatc cggcagtaat ttcggcgatg
    10921 ccgtaagaat tgcacgtcat tattgttatg aacattatcc ttacagcaat acctggggcg
    10981 cttatcaatg ttacggcgat caatttttta cattaaaacg cttaaactcc ggtaaaaaac
    11041 catcgctgcg caaatattat gttgccaatc aggtgagaat tgatcttgaa aatctgatca
    11101 atcgcattgg cgcaaattat tctgaaatac aaaaggctgc ttaccttgat gaaatcaatc
    11161 tgatactcga ctctgccgcc gcagccaata ttccacagtc ttcactgtat gaatgtctgg
    11221 gtatcattta caacagatta ggtaacgata gtgcagccat tgaaaatttc aaattatact
    11281 ttgaaaagtc cagtgaaaca tttaatgtta gcagctatgt agaatatgca cgattgttat
    11341 tgtcgcatta tacgcacgac aagctagcag ccaagaaaat acgtgttgta gtggcagaac
    11401 tggagaatct ttctaaaaca attccgtctt atgctatttt taatgccatc ggcacaggat
    11461 ataagattct tgcacgcatg agtaaaggtg ccgatcacag taaaaacatg ttgcatgcct
    11521 ttagagctta tgaacatgcc tataatagtg gtaaacaagc cggtgtagat aataccatgt
    11581 atgcattgag taatgccttt atgctcggaa tagcattaca ggataaaaag cttcaacttc
    11641 cggatatttc ggaattagaa tggtcgttca acaatattaa ggctttcgac aacacgttaa
    11701 cattttggga tttaatggat gaaccacgct attggctgtg caaattcgtt atgcaacatc
    11761 agggatttgt atttccacat ggcaatgtta aggtgaaagc ggaaactgta atagatgcct
    11821 gggaaaaggt cgtaaaaaca ggtggcatgg aaacgcaaca aaatgaaatg atcgctaagt
    11881 ttgcttcgat ggttacgttt tttgatccga agaaattttc aaagttacgc ccaaaactgg
    11941 atcagatgat ggacgctttg aagtgatagc atcagggcta actcggtaat taatccaaag
    12001 tatggcctgc acctaatccg ggcattaatt aacatctgct tttcattgag gctgatgttc
    12061 actagattta tgccctaaac aagcatttca tgcggaatct ggtatttttt atgcatgcct
    12121 ccttagatgg atttgttggt gggccacaag gtcaattaga ctggatcagc gtcaacgacg
    12181 agatttttga ttttgtggca actatgactg atgctgcaga caccgcatta tacggcagag
    12241 ttacttacaa catgatggaa agttactggc ccaccgcagg tgataaacca aatgcatcta
    12301 aacacgataa agaacacgcc gtttggtata atgcagtttc aaaaatcgtg ttgtcaacaa
    12361 ccattactgc cgaaggaaga aagaacacta cagtaatcag cgataacatc acggatgcta
    12421 ttcaggaaat aaaagctcaa gaaggtaaaa acatactcat ttttggcagt ccgtcggcat
    12481 cacatacact gcttaacctc ggacttatag atgaaatttg ggttttcgtt tacccggtta
    12541 ttattggaag cggcattcca ctgtttaaag acatacagaa aaaaacagca ctcgaactag
    12601 tcgaatcaaa ggttttttca aatggtggta tcgcattgca ttacagaatt agaaaataat
    12661 tcaacaaaaa aataaagttt acgaatagga ctcgctttat tgttttctaa tatgcctaat
    12721 tcatacgtaa atacatcgat gcaaattcgt aaacatataa aacatttata tgtctgaaag
    12781 tgaattaccc gaacacaaaa ccggaggttt taagatatta gaaattgttc cggaaatcat
    12841 tgctgccttt cagatcatta tttcaccgct attgattggc tcgataattg gcgcagtcat
    12901 ttatttaaca gagccctctc ctaccagact gctcatcggt atattgattg gcatattagg
    12961 tcttgtgatc ggcatcattt gggcggttcg tcaggcaaga cacaaaggag ctgcctggtt
    13021 tatgtcgcgc accattgcat cacccgaatt ggacgaaaaa ccatatgact tcaaaatcag
    13081 ctatcgtttt ttgagtgctg aggaaggtgg cagaaaaacc ggaccacctt ttcaaggtta
    13141 tcgccctgat tttgtttttg ccgtagacgc aaatgaaaat gttaaatctc ctgtagcatg
    13201 gatgattcat ccgatatttg ctgatgccga cggtcaagtt attgaaaaca aaacaacagc
    13261 tgtagccgaa gaaggcattg cttacatgcg ggtcattttt agggagcaca agcacctgta
    13321 tattgacaag atcaaagtag gcgaaaaagg atatatgata gaaggcccat ataaggttgc
    13381 gctctgcagt attatagaag tgtattaaca tcaacaaatg aaaatcagta acatacgaat
    13441 gaaaccaaat ctaattcaag catccatttg cacatgttta ataatgctag ggctttccat
    13501 gcatggtcag acaacatcaa aaggtccaca accaattatc acaaatggcg aagcccaaat
    13561 agttccggaa taccgggatc gacttgtgca agaactgtgg gttgaaacca cttttgattc
    13621 ggatggcgat ggtcgcctgg atagaatgca tacctatgta accagaccaa cagttactga
    13681 ttcatttgac ctcaaactac ctgttattta tactacaagt ccatattatg gattgaggct
    13741 ttgggcatta ctcgacctgt tcagaggaaa tacgtattgg aatgtcaaac atgagttggg
    13801 tgagcagcca aaaccacatc atcattccaa tctcggcacg agaacaaaac gtccgtttat
    13861 ggcattttat atggacaact tatggacaac tcgaggattt atcatggtgt attcttcttc
    13921 tccgggtacg ggactgtctg acggctctcc aactgttggc ggagagaatg aatcgctggc
    13981 acctaaagca gtgatcgact ggctgtgcgg aagaggaaaa gcatattcaa cacgcacagg
    14041 aaatgacgaa gtaaaagcat tttggtgtaa tggaaaagta ggcatgacag gcacctctta
    14101 tgatggcacc ttatgcatag ctgctgcaac taccggtgtt cagggattgg aagctatcat
    14161 tcctgttgca cctgttacat cattctacaa ttattaccgc tccaacggac ttgttcgttc
    14221 gcctgatggt tatttaggtg aggacatgga tgtgttgtac gatttcatta attccggcga
    14281 caagtcgaaa cgcgcaagaa atgatcgcga agtacgtgat agtattctcg tgaaaaatat
    14341 tgatagagtt acgggagatt ataatgcatt ctgggcttcg agagattatc tgaatgatat
    14401 cgacaacatg cattgtgcaa tgctgatggc acatgggttt aacgactgga atgtaatgac
    14461 agagcagagc taccgattct accttgcagc gaaagaaaaa gggttgcccg ttcaattata
    14521 ttatcaccaa caagatcatg gtggcgatcc accgttaacc atgatgaaca gatggtttac
    14581 acattatcta ttcggagaaa ataacggtgt cgaaaatgat cccccggttc agatagtcag
    14641 agaatttgaa gaattgccca caccttatgc ggcttttcct gatcccgatg ctgaaaatgt
    14701 aaccttgtat ttacaaggcg gcgttaacaa taccggcagt ttaattatag ctgcaaataa
    14761 caccctggtg ctcgacacac ttgttgatga ttatacaata agcggcgata tgcttataca
    14821 ggaaaaatac agcgagcatc gattgttgtt tgttacacca acactaaaga aagatatccg
    14881 catttcaggt atcccaagtg taaatatacg catggcaagt agcgcacagg cagcaaacct
    14941 atccgtatgg cttgtagcat taccttggga ggatgagaag aacacacaac tttatgaaaa
    15001 tatcatcaac agatcctggg ccgacccaca aaattataga tccatttcaa agggtgaatt
    15061 attgaaggtt ggcgaattct atcaggtgaa tttcaattta atgcctgatg atcaggtaat
    15121 tcctcaagga cagcaaatcg gattaatgat tttttcatca gacaaagatt tcaccatagc
    15181 accaaaaccg ggaacagaac tgacgattga tcttagcaga actaatatca ctattccttt
    15241 tgttggtgga gttgaagctt atgaagatgc tgtgaaataa aaaacgacaa catggaattt
    15301 agtccgcaaa atgctatcgt tcgactttgc ctagccggga tggaacaaga aaccaacgga
    15361 gatcccctag ctgcaaagac cttatttatg gaggcctgga gtacatcgac aaatgatttc
    15421 gaaaaattca tctccgcata ttacatagca cgtcatcaaa cttcgcctgc agataatctc
    15481 cattggctgc aattggcgat ggaatttgca ctatcagcga ataccctctc tgcatcaagt
    15541 gcattgccga atgtatatgc taaaatcgca tcctgttata aggaaatcgg cgatgatgca
    15601 cagtcttcaa aatacaggca gcttagtgaa gaaagcgcac atcaacttcc tgacaaaggt
    15661 ccattttatc atggcacacg tgcagatttg caaaccggtg atgatttgtt acccggtaac
    15721 aggtccaatt atcagtcgga cattatcatg aatcatattt atttcacagc tttactcaat
    15781 ggagcgggac tagcggcagc attggcaaaa ggcgaaggca gggaacgtgt ttatattgtt
    15841 gaacccaccg gtgatttcga gaacgacccg aatgttaccg ataaaaaatt tcccggcaac
    15901 cctacccgct cctatcgcac gacatttcca ttgaggataa ttggcgaagt aatggaatgg
    15961 gaaagacaaa caccggagca gatacaacaa tggaaagaca ggcttgcaaa aaattcaggt
    16021 gagatcatta attgaaaatt catagttcaa aggaagatgt atcatttttc atattttaaa
    16081 gaaaaggacc gggcgactat tatcgacttc atgcataaac atccattcgc attcgtgacc
    16141 ggaagttttc atgatggcag acaggttgct acgcaagttc cgttattgat agaagagcgc
    16201 aatggtgaat tggtattgca aggccatttt atgcgcaata ccgatcatca taaagcattt
    16261 attgaaaatc cgaatgcctt aattgtattt accggaccaa gtgcctatgt aagtgcaagt
    16321 tggtattcca atccacaaat tggttctacc tggaattaca tgagtgtgca tgttacaggg
    16381 cagatgacat ttatgtcgca cgagcagctt attcaactca tgaagaaatt aaccttacat
    16441 tttgaaaaag agaatgctgc ttcgccaacc atttatgata acctgccgga tacatttttg
    16501 gataaaatga tgcccgccat cgttggcttt gaagtgaaag ctcaaaaatt agagaatgta
    16561 tttaaactta gtcaaaacag agatccacag agctatgata acatcattat gcagcttgaa
    16621 caactcggtg gtgaaagtat gttaatcgct caggaaatga aaaatcgacg cggtgaatta
    16681 tttgctgaag gcagcgaatg ggatccaaat aaatttgctt cgtaagttat gacaaatgaa
    16741 catgaaatag taagcagcag gatcatttct gcttccattg ataaactata tcaagctttt
    16801 gccgatccgg aaaaattagc ccaatggtgg ggaccgaaag gatttacgaa taccattcat
    16861 gcctttgatt tgcgcgtagg aggcaaattt gaactaacga tgcatggccc tgataaaggg
    16921 aattatgaga acgcatctgt atttactaaa gtaattcctt taaacctaat ttcctggcac
    16981 cgcatcaccc aaccgtggtt tgacatggaa gtaatctttc aagatattga cggcaattca
    17041 tcaagcataa catttcgcat gattttcgac tcagcagaat cttgccaaaa actccgccgc
    17101 tttgttgaac ccaataatga agagaatttt gatagactgg aagcaatgtt agcgacttcc
    17161 acattataag ttgttcatct tttgaactcg gatctcgctc agtcacagac ttttcttcca
    17221 ccttttgaag ccaaaaggtg gctccaaaag ctcgaggctt ccgcaggcga atgacaccgc
    17281 cctagtgctt gcgttatggc ccgaaaaagg tcggatttta caaagtaatg agctaatttc
    17341 tttatctaat taagagtagc gatttaattt ttagtaatta acgactgcct tcgggcttac
    17401 ctgtcgttta gcaactcgac atacagttaa acacagtgcg tagagactgt tgtcatcgcc
    17461 ttgtaatccc tcatcgttgg aactaagatt gttcatgaca tcgtaatgct gtggttttgg
    17521 catatattat ctctgataag tagtcgatga tattcgcaag gttgcttaga ttgatctatc
    17581 atttatttta ctttcccttc aaaagcaact tacatcgctg tctctgcggc tccgcgcctc
    17641 tgcgtgaaag caacgtgcca taacaacaaa ccacagcttc gcgccttcaa acctttgcga
    17701 ctgtgcgcga tacaaacaca aaccacttaa acctatttac atatcatttt aatttaaata
    17761 ttatacctat ccaatctcaa tatattttta taacttcata ccgaaaatag tcccgcgata
    17821 accttctaag aagtgatcaa aactcaatta tccgaaaccc tatgaaaact ttaatgacat
    17881 tcctattaat cacagccgga ttcaagtgtt tttgtcaaga ctgggcgttg tttccgcacg
    17941 gcacagacat gtattatttc gaggaaggca ccactaccct tttcagttgc aaacaggact
    18001 ccattttagt agcaggagat actactatct attacttcaa taaaattgcc cccgaaggag
    18061 ctactcaggc ttgctatgat tcaattttgg attatgacga atatactatg tttagttggc
    18121 cttcaattaa aatggaattt acaggagttg attacaactt attcttttac tgtgatgaat
    18181 tcggggcatt gccggcaata ttcaaatctc ttgctgctgt tgggtcaaac tgggaaatac
    18241 ctaatgcctc agattatagt gagttttcta ctgtcacaat aacttgcgat tcaattatct
    18301 tgggtagttt tctagggatc actgacagtc ttaaatatta ttctatacaa accgttgcgc
    18361 ccttaacagg cacctatacc attgataagg cgcaatacat cctcagtaag cataatggac
    18421 ttataagatt tctgcccttt caaaagctac taaatccaac tacatgcgaa gattgcttta
    18481 aatgttatga tttggtaggt caacattcag atatcggaac aattggatgg agcggtcctt
    18541 tatgggagaa ttggatacag ttgtctgaag gcgattttct taaatacaaa cactattcaa
    18601 attctatctt tggcacaaca gagtcgtata caactgcttt gatacaaagc gtacaacact
    18661 ttagtgattc aattgtaatc gagtcattaa acgcagatgg attgttaggc acaagcacat
    18721 attataaagc tgcaatattt aatgccatta atgctcctgt ttggcaacca ttttatattc
    18781 ctgaatatca caatcaaccg gagttggatc gccttattgg aatagcagat tcatatgaaa
    18841 caattgtcga taccactacg tttccgggtc attggcgtta caagcagagt ttggtaactg
    18901 atgccgtaca ggataatgat ttctgttatg ttgaaccttc gtttgaatac ggccatgaaa
    18961 tcagatggga ttcctatatt ggatggactt tcgaatcgag cggtggtcca aaatactata
    19021 actactctct tttaatagga tatgtaattg acggtcaaac ttatggaaat tactggccac
    19081 ttacagctga agaactggct atgcagaaac ccttgctctc aatattccct aatccggcaa
    19141 ctaatacaat cacggtcaat ttaccgcttg aaacattttc gcaatacgaa atatgtgata
    19201 tcaccggcaa gatatgttct agcgggacta tgcaaaacat gtcacaaaca atcgatattg
    19261 gagatattcc ttcaggcaca tacattctaa aggtcgcctc cgatcatgcc atccaaacag
    19321 gaaagtttgt gaaattgtaa aatcgtaaaa tcattttgca tcgggtagcg caattattaa
    19381 ttaggtggct tatgcatgtt gacttgcaag ccatttacaa aatatgaacc ctcaaatttt
    19441 ttcgttaata tttcacaatt gatcattcgc atgcaaacct ctgctaccac tcccctgctc
    19501 attctcgttg cgggtcctta tcgctcaggc accaacgatg atccggcatt gatcgccgca
    19561 aatgtaaaag cgatgactga tacggccctt gaattatacc gactcggtca tatgcctgtg
    19621 cttggcgaat ggtttgcatt acccttgatt gaagctgcag gttctaagca aattggtgat
    19681 gccattttca atgaattatt tcatcctgtc gctgtaaaat taattgacca ttgcgacgct
    19741 gtattacgca tcggcggacc atcggcaggt gctgatgaaa tggttaatac aggtaaggcg
    19801 aaaggtaagt tgatatttat ggaaatgaag gaaattcccc aactcaacaa taaatgattt
    19861 gatcacaatg cagtgatcac gatacctaat tcacgcaact cttcaacggt aattgtaggt
    19921 ggcattatac cctgttccag ttttttgata gcaagttctc ccgacttgta ggcatcactc
    19981 tccgatgcaa atggatgcag accattaata gcaggaatat gctcctgatg cacgtagcga
    20041 acagtgtcga tcaggatatc ataaccccat ccaccctcta cctgaaatgg aatacaagtg
    20101 tattgtgttg aagaggttgc agtaggtgac gatttggaag catgcgtttc atcaatcggc
    20161 ctcgtattag aactacatgc tgttatcccg actatcagta acatcgccgg taggcaacga
    20221 aatgtttgca tcattttatc acaacaaatt ttgtggcctt gtgtgctttg ccattatgca
    20281 ctacacactg atacgtacct gcagggagtg catgcacatc catttttccc gtatagtggt
    20341 ctgaagtaat tcccgaccat gaaacaatgc aatcgcgtgt cacattactt ccattcaaat
    20401 cgaatatttc aattattaaa tcatcgccat cctgcatctg ttgtatatca atattggcat
    20461 atgcagtaac cggattcggg aataattgca ctgttgcctg caatgcatgc aaatcggtaa
    20521 cagcatcttg tgtatcaacc ggaacaatat tcgtggtatc gccccacaac cagatatcat
    20581 aattaaatga agtatcatat cccatgccta caaatgcaag tgtatcaatt acaaaagctg
    20641 tcgcattcac acgcgcgccg cccggaaaat caggcatttg tgtccaggtg tcggcaatag
    20701 gatcatattc ccaaaaatca tctctattta ct
//
LOCUS       DAOQOU010000042        19515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1394_length_19515_cov_6.160894, whole genome shotgun sequence.
ACCESSION   DAOQOU010000042 DAOQOU010000000
VERSION     DAOQOU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19515
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1394_length_19515_cov_6.160894"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLM90_05595"
     CDS             <1..307
                     /locus_tag="PLM90_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ88876.1"
                     /translation="HLVAVSEFLEEYFTSREALGEKMEEAAETIRKSGLKCTTEMIRH
                     DMVNNSIKIYASEIDADLLVIMSHGENKFNQLIFGSRINKVISHSDIPVLSFRPEDD"
     gene            complement(314..2005)
                     /locus_tag="PLM90_05600"
     CDS             complement(314..2005)
                     /locus_tag="PLM90_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88877.1"
                     /translation="MHAFSLSNSISINTDIDKACKNAFPTLIATDIRLANTEHSPKAD
                     YYYFNQYYYNIPIAGAGVKWIVPHNGDTSWLFGNIYDTINMSFLEAEISVRFDLIHEQ
                     LNRLGVTEHIDESKCWYISGNTLSLHRTYSYRFPDGVTYTFHFNRAGELIATEDNRSY
                     FTLPDTTVAGKVFLPDPLTTAGVEYGDDYVDEDDADLTVLNDQRFDVRVPAQLIDGIF
                     YLRTDSLLLKDINIPVIPIASATTDTMVFTRSESGFEDVNTMYHITNMYQYLASIGYE
                     ALHNFYLEVDPHGASGADQSFYTGLPFPSIQYGEGGVDDAEDADVIIHELTHAFSDHA
                     SPNTNTGLERRAVDEGYGDYFAVSYSRTYSDFLWKNVFCWDGHNEFWFGRNTDTDKHY
                     PEDNNDNYYASSEIWSGALMDIFDLIGKEATDRIVCEALYGSFPNMIMPDAAQILLNA
                     EMMLYGGIYHDIMFDALYQRGLVWPVDVVQQNFDNIRITNTLGYASGNEPIFITLETP
                     GNGSWQLFNLDGKLIAEKSFYGSSCAIDVHPTDGQLLLLKVNAQGKTVSTIIANF"
     gene            complement(2052..2666)
                     /locus_tag="PLM90_05605"
     CDS             complement(2052..2666)
                     /locus_tag="PLM90_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HMZ88878.1"
                     /translation="MEAPETYITILQSATGSYSESGSKFLGYSFAIVDESEIKILRDQ
                     LSKEHHKAVHVAFAARLGFEGENERSSDDGEPSGSAGKPILNELKSRVLTQTCVLVVR
                     YFGGKKLGIPGLIHAYRAAAIDCLNNSGTKEVVLYDDYEISCHMDHMPVVLHELNKSG
                     ARVIDSEFAENCNFKIEIPRTQHAAFTKTLQNLWQATATYIGMH"
     gene            2805..4052
                     /locus_tag="PLM90_05610"
     CDS             2805..4052
                     /locus_tag="PLM90_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ88879.1"
                     /translation="MNTTAYISIIADMSDRITTLRTANEAQAYFSEQFRKMFPITHCA
                     MYRVSERDDTFYRTYDFAAPKANVQPKAGDKITLSKAISDDKDTFVILHHQEPWFVVP
                     RQQVKQKGEVLLLIRNAEKVVAIIHGIAPEPIPENGFQNDTARVFSNFFIQVEGGLQL
                     RTQINEHQIELSGHIANNNDTLFQLNERLIQYNEELQQFAYSASHDLQEPLRNIASYI
                     NLFIKQYGSTINEEGLEYLQFASDGAQRMQQLIRDLLTYNKLDQQQEPMALFEGNQML
                     QEALYNLRMSVEESNALILYPELPVLYGNPSQIILLFQNLIDNAIKFRKNDEPLIFIE
                     MEEKPEHWEFKVVDNGMGIAAQYQQKIFGVFYRMHGPDQIRGSGLGLSICKKIVERHH
                     GTIKVDSKPGKGTTFIFSIGKNQ"
     gene            4077..4943
                     /locus_tag="PLM90_05615"
     CDS             4077..4943
                     /locus_tag="PLM90_05615"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HMZ88880.1"
                     /translation="MKLLFNLTVVLTLACIMSCSDSTNTVEKVNIPAAKGKLFIIGGG
                     DRGDSLMQAMLDAAGWEKGDLITAITLPSGWGDSAYIWLNNDFKKLTGQNCVKFDSAA
                     VHVPAKLDSLRMSKVIFISGGDQNKMMQLIKGTDIKKAIKEAYMNGATISGTSAGASI
                     QSEKMITGDGLIDTVYDVTFDVLWNGNIELVEGLGLLDSVIIDQHFVVRSRHNRLLSA
                     VIENPGYQGVGIDEGTAILVSGDTATVYGASQVLTFTNPASITSPKDSLLNGRDIRVS
                     IYLPGEKFAIKR"
     gene            complement(4944..6371)
                     /locus_tag="PLM90_05620"
     CDS             complement(4944..6371)
                     /locus_tag="PLM90_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88881.1"
                     /translation="MENTNLQQLLQTLTKSEWREFGDFLSSPFFNKNEKFIRFYHQIN
                     PYQPGFVISEPEKQRIYIETTQSKAWNDAGYRNICSDFLELLQSFITQLVIASGSSQK
                     GFYLNMELLQRGQLALVEKNLKKISSYLKTDSIDIGERLRTEVWLADLHTLLNIRMNR
                     SRIHHASQFMINDTSFRTNTELLLLKVFITFHNYITATQNQNEPFDKKRIKPFLQLYE
                     AFEPFEHVEVALNYFLLRMVSEGADDNYYKTKELFISVLDKLHNKDKSNMMTSLLNFV
                     NGKVGSDESWREEVFALHDLKLTKRLWSNHSDLGYVSLFTAVQNALQMNRITYAEDIL
                     EQYSPFLNHVIRDSIYHLSKGFIALFKGDYSFAHQCIQKVETENNLLKYEIRVLQLLV
                     YFEQKEWNLLASALESFRQFIQYNKPNISPEITDQYLTFVKQMQQLMKLPPAPSQKQR
                     DQLIKTLRTDGHAYFKSWLLRKAQN"
     gene            6419..8260
                     /locus_tag="PLM90_05625"
     CDS             6419..8260
                     /locus_tag="PLM90_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88882.1"
                     /translation="MALWNKVTLQQHQLYQHAKHVMTAENMEIRNLKNIIMKKQLFTS
                     IFTLSALFGVAHAQTNTFPTTGSAGIGTITPASSAILDIQSTTQGMLAPRMTKAQRDA
                     IVSPTTGLLIYQTNSSPGFYYYTGATWSAMAAKGANTSLSNLAASTSINTALTPNANN
                     TLDLGSTTNNWNELYVNTIKFMDGTTQITAGGGGGGGVGGSGTISYLPKFTSATTLGN
                     STIYDNGGNVGINTTTMVGSANFVSKSNSVTGYGGMYVEMGGTSGRKPFYGYSLSGIA
                     KAWTYFDEATNQYRVNNNGDRMVIDNSGNVGIGTTTPSNLLHVETSGTVTPVKFVKPW
                     TGTGTTDFFLLEVSNNYNFGYGSGINAYGGKMGIKGSAFNGDQIGYGVYGYGFGGTGT
                     TYGVYGTAGSSGSAIGVYGTTTSGSSQWAGYFVGRGYFSDNLGIGTSTPSTKLHVIGG
                     TDVELATGGFATFGATTATNIAIDNNEIMARNNGATSTLYLNNDGGNVSMCYASGSVM
                     IGASVPATGYLLSVDGKVMCEELKVQMSESWPDYVFADNYKMPTLYELEASIKANKHL
                     PGIPSAAEVAENGISVGEMQTNMMEKIEELTLYIIELQKQIDELKQQ"
     gene            8285..10579
                     /locus_tag="PLM90_05630"
     CDS             8285..10579
                     /locus_tag="PLM90_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HMZ88883.1"
                     /translation="MKKLLIISGIAWMCSIVNVKAQSMALPLQITNKYQLSIEPGSTR
                     EWISFRRDAPFQALTIFEQAPDLLGLREQDEFRKFSERTDAAGNKHYKFAQYVNGVRV
                     EGIEYYTHERNGRFHLMNGDVVNNLQLSSTPSISKEQAIASALAQFPATKYMWEDPRA
                     EAAFKQKNNNPNASLYPDPELLFVKIDPKGSQVADNYILAYRMYVFAVEPHIAEYVYI
                     DATTGAFLRNRSLEISCNPSSMATTFNGTQTVNTDYRTEDCGSTYTTETSYFSIDDCT
                     PGSEIRSYFSASYGAWNYGDDWLLCDNDNSWTNTGSYLMVMTSLWSTRKAMNYYLNVH
                     GHESFDGSGGLIDLFSNKTYFDDDDLPYCRNANYTNIIDNLNFGSGNDCMPGTMDDYN
                     TLDIVGHEFTHGVIEYAHFDALDYSDESGALNESFADIFGEMVEYYTEGGDTLSWLCG
                     EDRGAIRSFKNPKAFGDPDTYYGTNWATLGGTDNGGVHTNSSVQNHMFYLLSQGGSGV
                     NDHGIEYQVEGIGYDHARDIAWVAMMDYLDGSDGYIIARNAWIQAAIDLFGSCSQEVI
                     SVGQAFQAAGVTEYTAFDRASVCGTYVLSGYADATYGIENSTMVLGDFLIDCTTTINA
                     TAAVTFKSGYYIQLNPGFTALSGCNLTAYISACEISEYDPDNVRIAQSNLQSPTVAEM
                     DDKLLVFPNPVFDIVTINWEREIPMEQLQMVNIAGQNVTDHISIEENTLGKAIIDMRN
                     LAAGMYMIVIKTADGEAYGNCIKQ"
     gene            complement(10650..12752)
                     /locus_tag="PLM90_05635"
     CDS             complement(10650..12752)
                     /locus_tag="PLM90_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMZ88884.1"
                     /translation="MKKCLLPALLFLVASAGQLNAQQAIPPKAERIQHTIHGSFGDRE
                     DPYYWISDRDNPKVIDYLKAENAYTEAVTAPYKKIQEQLFKEMVGRIKEDDNSVPYKK
                     GNYWYYDRYEKGGEYPIFCRKKGTLDAKEEVLVDGNELAKGHNFLSYEVEVSPDETKA
                     CIIMDVHGRNFYTIRVRDLQTKQWLSDEVPGTRNGCVWSRDNNSFVYGVPDTVTLRVY
                     QVKRHFLGMKASDDKLLFEEKDPTLDIYVYGSRSEDFIFAYSGRTDANTVWFADARDP
                     RQFKQVAPLVENVVFNVDHVKGEEFTILTNYKAKNYRLCSVNYFDPRMDNWQDVIPGR
                     DDVFLTQVQYFSNFMVAEEVQNGLTMLRVIVNDKTDVPIKFDEPAYYATLGYNPDYHA
                     TGFRYHYSSLITPFTTYHYDVSSSLTEILKQQEIPSGYKKDLYKTERIMVTARDGKQV
                     PLTISYRTDMFHKNGTNPGFIYGYGSYGYSNEDYFDANILSLLDRGFVYANTHIRGGQ
                     EMGGEWYDDGKMMNKKNTFYDFIDCSIWLQKNNYVASDKLFANGGSAGGLLMGAILNM
                     APTTYKGVIADVPFVDVITTMEDESIPLTTFEWLEWGNPKIKEQYDYMMSYSPYDNVT
                     AQNYPNILVTTGLNDSQVQYFEPTKWVAKLRYTKTDKNMLLLKVNMDAGHGGRSGRFE
                     SVNEVAFMYAFMLQCLGI"
     gene            12882..15947
                     /locus_tag="PLM90_05640"
     CDS             12882..15947
                     /locus_tag="PLM90_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88885.1"
                     /translation="MQKFLFLALFSLAGMQLFGQGRTITGRITDADNGVYLAGVTVLL
                     EGTMNGTVSDAEGKYSITVPEEGGNLIFSFIGYENQKITLGTKNVLYVSMAMSQEILD
                     EVVVVGYGTEIKTKVTGSISEVGNDEIALTPVSTVEQTLQGRAAGVFIESNNGKVGSD
                     VRIRIRGNSSINASNQPLVVVDGVPINTVGVNDYFNVVLNPMNDIDFNDIESVEILKD
                     ASAAAIYGSRGANGVILITTKRGKVGKPTVTLDYQYGFSKPTRLREFLNAQEYIDYYT
                     QAAINAGEYDFTNGLSGYTSVEEAIDDYIADAEKKFNKLQGYTDWQNDYANTDWQSLA
                     FQEATSTMFDFSVSGGSEKMQYFTSVGYNYQDGIMIGNHGDRFNITNNMDAQLTEKLK
                     LGTSLTISRSINNDIPDDNSFSTPLQMVAQSPLTPPQDTTGEYYNTPTTLYYNPLIEV
                     DNVFFESTTFRTIGNLSAEYQLRPVLALRAELGGDITNHWQQRFYGSKSEVGLSVGGY
                     ATNFNSTVQYFDTKMLLLYSQKFGKSTLGATTGVEYNQYDDQYNSVEGQGFPSDKLQT
                     LASAAEIVYGTGVLNAYRFLSYFTRITYDYDTKYLFNVTVRADGSSRFGENKRYGVFP
                     SGSFGWVVSNEDFLKGNKTLSFLKVRMSYGMTGNAGIGNYDYKGLYAVGSYAGTSALY
                     PSTIANPDLTWEKTTQADLGIDFGFINDRINGEFDVYLKQTTDLLLDVPVPSTTGFTV
                     QTQNVGSLENKGVEFTINSDNFVRTFTWSTSVNFSANRNKITELANGQTIIDYGGSDF
                     MNVVMVGQPLGVFYGAEYAGVDSENGDGLWYVNDGTDATTNDFNDANFVVVGDPNPDF
                     IAGLTNNFGYKNLSLDVTLQGVYGNDVHMSGDYWMSSNASQWDNQTVDQLNAWQNPGD
                     VTDVPENRLLFENGTAARSSRYLDDASYLRVKTVTLSYDMPKRVTGKLNMSAMRLYAS
                     AYNLFTVTNYRGWDPEVSSDSFVDNIYYGIDFYSAPQPRTVVFGISLGL"
     gene            15977..17317
                     /locus_tag="PLM90_05645"
     CDS             15977..17317
                     /locus_tag="PLM90_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ88886.1"
                     /translation="MKKNILYTLCAIILIGGLNSCEDILVLEPAQSLSNETALDNDLG
                     VKQTLVGAYDRMAQSSLLGGDVIMNADLYSGAGEFEWVGTYVDYREIFTHDILVNNGS
                     VSSLWDDAYEVINTCNNVINAIDVVEAADQDRVRGEALTLRAWCYFELVRAFGYQYQP
                     GVENTQLGVPLILTPTLNFGDNTNVTRNTVEECYGQIIGDLEQAATLLPEKNDVYVNT
                     YTASALLARVYLQKEDYENARDAADRVIASNDYELMSNVADVFNQDDATDEDVFSVEI
                     STTDGNNSLNNYYATNDFGGRGDIEIQDAHMALYDPLDARATLFFIDGGIRFTSKFNN
                     EFGNISAIRIAEMYLIRAECNQRLGTSVGATPEDDYNAIHTRAGLPAAASVSLDDILY
                     ERHLELCFEGQAIHDIKRLHGMVGTLNYNDPKLIYPIPQTEIDRNALLVQNDGY"
     gene            17601..17987
                     /locus_tag="PLM90_05650"
     CDS             17601..17987
                     /locus_tag="PLM90_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88887.1"
                     /translation="MKKLLMPVLLAAGLVFATAGYSQDAVKFDQHFQVQLTDAMLQSG
                     SVVMDISDLQFTDEAAAQKYFNAIRNNLVEFTLDYSAHTVTMHLYPERLGKHTWTLSD
                     WNAYLANMAERNAETYQSFQISSPKQ"
     gene            17993..19273
                     /locus_tag="PLM90_05655"
     CDS             17993..19273
                     /locus_tag="PLM90_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88888.1"
                     /translation="MKKKILQILPAALLITGVMQTGHAQIVSGVSFLKGKWIELAVSD
                     CGTYASGGAGMIVAAPATYHENTIDGSLSFGNDVTADGWDIGVPDQCGDFIMPGSPEE
                     GFAIQIGTGPVYGNLQPYCYRFGAFVAAAPDFVGTNLTNVNGGSIRKTLWQGTNTGIG
                     LAVSQTTYWLNSKQSYITIVDLCNGGDDMFDVYYARNADPDNDQITSGTFNTNNNAKK
                     QYATAGYSQVTATSTVGSPCYMAYVSNDSRAKVSRGNFSMGSPSDIWNGLSGYIQTPG
                     AVNADAAIQISFKIDTLLHNDCGCFTYSTMLTSAGLTDQLALTGTACATLGTLARYGE
                     DMIDGYLNDPTNFLKNEMVVYPSPSNGNFTVNIFEMEHANITVVNAVGQVVYQVNDVS
                     KIAGITLDNPVPGMYFVNAEYGEGKKITRSLVIE"
BASE COUNT         5769 a         3827 c         4161 g         5758 t
ORIGIN      
        1 acacctcgtt gcagtttcgg aatttttaga agaatatttc acttcgcgcg aagcccttgg
       61 cgaaaaaatg gaagaagcgg cagaaaccat ccgcaaaagc ggcttaaaat gcactactga
      121 aatgatccgt catgatatgg tgaacaattc catcaagata tacgcatcag aaattgatgc
      181 cgatctgctg gtgatcatga gtcatggaga aaataaattc aatcaactca tttttggttc
      241 tcgcattaac aaagtgattt cgcactcgga tattccggtg ttgagtttta ggcctgaaga
      301 tgattaagcc gggttaaaaa tttgcaatta ttgtcgaaac agttttcccc tgagcgttta
      361 ctttcagtaa taataattgt ccatcggtag gatgaacatc tatggcacaa gagctgccat
      421 aaaatgattt ttctgcaatt aatttcccgt caaggttaaa tagttgccat gaaccatttc
      481 cgggagtttc aagtgtaatg aatatcggct cgtttccgga tgcataccct aaggtattgg
      541 taatgcgaat gttgtcaaaa ttttgttgaa caacgtctac aggccacact agtccgcgct
      601 gatataaggc atcaaacata atatcatgat aaattccgcc ataaagcatc atttctgcat
      661 tcaataatat ttgtgcggca tccggcataa tcatattcgg gaagctgcca taaagtgcct
      721 cgcaaacaat cctgtcggta gcttcttttc caattaagtc gaaaatatcc atcagtgcgc
      781 ccgaccaaat ttcgctgctg gcataataat tatcattgtt atcttcagga tagtgtttat
      841 cggtatctgt atttctgcca aaccaaaatt cattgtgacc atcccaacaa aaaacattct
      901 tccataaaaa atcgctgtat gtgcgagaat agcttactgc aaaataatcg ccatatccct
      961 catcaacagc gcgtctttca agtccggtat tggtatttgg tgaagcatga tcgctgaaag
     1021 catgggtaag ctcatgtatg attacatcgg catcttcggc atcatctaca ccaccttcgc
     1081 catattgaat acttggaaat ggcaatccgg tataaaatga ttgatctgca ccggaagcgc
     1141 cgtggggatc tacttcaaga taaaaattat gtaatgcttc atatccaata gaagccaaat
     1201 attggtacat attcgtgata tgatacatgg tattcacatc ttcaaatcca ctttcgctgc
     1261 gagtgaaaac catcgtatct gtggttgcgg aagcaattgg aattaccgga atatttatat
     1321 cctttaacaa caacgaatcg gtgcgcaaat aaaatatgcc gtcaatcaat tgagcaggga
     1381 cacgcacatc aaagcgttgg tcatttaata cggttaaatc cgcatcgtct tcatctacat
     1441 aatcatctcc atattccaca cccgcagttg taagtggatc agggagaaat acttttcctg
     1501 ctactgttgt gtcgggtaaa gtgaaatagc tgcgattgtc ctcagtagcg attaattctc
     1561 cggctctgtt aaaatgaaag gtataggtta cgccatcagg aaaacgatag gaataggtgc
     1621 gatgcaaact gagtgtgttt ccggatatat accagcattt tgattcatcg atatgttcag
     1681 taacaccgag tctgtttagc tgttcatgaa taagatcaaa ccgcacacta atttcggctt
     1741 caagaaatga catgttaata gtatcgtaaa tattaccaaa cagccatgat gtatcaccgt
     1801 tatgtggtac aatccatttc acccctgctc ctgcaatggg aatattataa taatattggt
     1861 tgaaataata gtaatcagct ttaggactat gttcagtatt tgccaatctg atgtctgttg
     1921 ctattaaagt tgggaaagca tttttacacg ccttgtcaat atcagtgtta atagaaattg
     1981 aattactcaa ggaaaaggca tgcaagcgat tgatattcaa ctgcgcgaag gcattcctgc
     2041 tattaaacag attaatgcat accaatatag gtagcagtag cctgccaaag gttttgcaat
     2101 gtttttgtaa atgcagcatg ctgagtgcgc ggtatttcaa tcttaaaatt acagttttct
     2161 gcaaactctg aatcaataac acgagctccc gatttattta attcgtgcaa aacaacaggc
     2221 atatgatcca tgtgacacga gatttcgtaa tcatcataca ataccacctc ctttgttccg
     2281 gaattattta aacaatctat tgcagcggct ctataggcat gaataagtcc gggaattccg
     2341 agttttttac cgccaaaata tcgaacaact aagacgcagg tttgagttaa caccctgctt
     2401 ttcaattcgt taagaatggg tttgccggca ctgccggatg gttcgccatc atcgctgctt
     2461 cgttcgtttt cgccttcaaa acctaatctg gcggcaaaag ctacgtgaac ggctttgtgg
     2521 tgctcctttg aaagctggtc gcgaaggatt tttatttctg attcatcgac tatagcaaaa
     2581 ctatacccca aaaacttgct tcccgactca gaatagcttc ctgtagcgct ttgcaggatg
     2641 gtgatataag tttctggcgc ttccacaggg caaagttaag ctttcgtgtt ttcttcgttt
     2701 ttttatttag aaaaacataa aaaaaggcct tcgtttggtt aaaacttgtc taacagtttg
     2761 gtgatttcag gctgtaaggg taaaattgta ctacacacga cattttgaac acgacagctt
     2821 acatttccat tattgccgac atgtcggacc gcattactac gctgcggact gccaatgagg
     2881 ctcaggcata tttttcggaa cagttccgta aaatgtttcc catcacacat tgtgctatgt
     2941 atagagtgag tgagcgggat gatacttttt atcgcacgta tgattttgca gctccaaagg
     3001 caaatgtgca acccaaggcg ggagacaaga ttactttatc aaaagcaata agcgatgata
     3061 aagacacttt tgtgatcttg catcatcagg agccatggtt tgttgtgcct cgacagcaag
     3121 taaaacaaaa aggtgaggtg ctgctattaa ttcggaatgc tgaaaaagtt gtggccatta
     3181 ttcatggcat agctcctgag ccgattccgg aaaatggatt tcaaaatgat acggcacgcg
     3241 tcttttctaa tttttttatc caggtagaag gtggtttgca attgcgcact caaataaatg
     3301 agcaccagat cgaactttca ggacatattg ccaataataa cgacacatta tttcaattaa
     3361 acgagcggtt aattcaatac aatgaagagt tgcaacaatt tgcatattca gcatctcacg
     3421 accttcagga gccgctgcgg aatattgcat cgtatattaa tttatttatc aagcaatatg
     3481 gcagcacaat aaatgaagaa ggtctggaat atctgcaatt tgcatctgat ggtgcacaac
     3541 ggatgcagca attgatacgc gatctcctta cttataataa actcgatcag caacaggagc
     3601 ctatggcctt gtttgaaggg aatcaaatgt tgcaagaggc attgtataat ttgcggatgt
     3661 cggtcgaaga aagtaatgca ttgatattat atcctgagtt gcctgtgtta tatggtaatc
     3721 cttcacaaat tattttattg tttcaaaatc taatagacaa cgctatcaag ttcaggaaaa
     3781 acgatgagcc cctcatcttt attgaaatgg aagaaaagcc tgaacattgg gaatttaaag
     3841 ttgtagataa tggcatggga attgcggctc agtatcagca aaagatattt ggcgtattct
     3901 atcgcatgca tggccccgat cagatcagag gatcaggatt aggcttgtct atttgcaaaa
     3961 agattgtcga acgccatcat ggcactatta aagttgattc caagcctgga aaaggaacca
     4021 cgtttatttt ttcaatcggc aagaaccaat aactttatcg cgataaatta ttattcatga
     4081 aactattgtt caatcttaca gttgttctga cacttgcgtg catcatgtct tgttccgatt
     4141 cgacgaacac tgttgagaaa gtaaatatac ctgcggcaaa aggaaaactc tttattatag
     4201 gtggtggtga cagaggcgac agtctgatgc aagccatgtt ggatgctgca ggatgggaaa
     4261 agggtgattt gatcacagca attaccttac cgagcggctg gggcgacagt gcctatatct
     4321 ggttgaacaa tgatttcaaa aaactgacag gacaaaactg tgtaaagttt gactctgctg
     4381 ccgttcatgt gccggcaaaa ctggattcgc tgcgtatgtc aaaagtgatt tttattagtg
     4441 gtggtgatca aaacaaaatg atgcaactga taaagggcac tgatatcaaa aaggctataa
     4501 aggaagctta tatgaatgga gccaccattt ccggtacaag tgcaggagca agtattcaaa
     4561 gtgaaaaaat gatcaccggt gatggattga tcgacactgt gtacgatgta acctttgatg
     4621 tgttgtggaa tggaaatatt gaacttgttg aaggtctcgg tttactggat tctgtaatca
     4681 ttgatcagca ttttgttgtc aggagcaggc ataatcgatt attgagtgca gtgatagaaa
     4741 atccgggtta tcagggcgtt ggtatagatg aaggcactgc tatactcgtt tccggcgata
     4801 ccgcaacagt atacggagca agccaagtgc tgacgtttac aaatcctgca tcaattacat
     4861 cgccaaagga tagtttattg aatgggcgtg atattcgggt ttctatttac ctgcccggag
     4921 agaaatttgc catcaaacgc tgatcaattt tgcgcttttc gcagcagcca tgatttgaaa
     4981 taggcgtgtc catccgtgcg cagtgtttta ataagttgat cacgttgctt ttgtgagggc
     5041 gcaggcggaa gtttcattaa ttgttgcatc tgcttgacaa aggtgagata ctggtcggtt
     5101 atttcggggc tgatatttgg cttattgtat tgaataaatt gcctgaaact ctccaatgca
     5161 gaggcaagta aattccattc cttttgttcg aaatatacta gcaattgtaa aacacgaatt
     5221 tcgtatttaa gcagattatt ttcagtttcc accttctgaa tgcattgatg tgcaaatgaa
     5281 taatcgcctt taaacaaagc aataaatcct ttcgataaat gataaatgct atcgcgaatt
     5341 acatgattca agaacggtga atattgctcc agaatatcct cggcataggt gattctattc
     5401 atttgtaagg cattctgcac agcagtaaac aaactgacat aacccaggtc actatgattc
     5461 gaccacaatc gttttgtaag tttcaggtcg tgtaatgcaa atacctcttc acgccagctt
     5521 tcatcggatc caacctttcc atttacgaaa ttcaacaagc tggtcatcat gttacttttg
     5581 tccttgttgt gtaacttgtc cagcactgaa ataaataatt ccttagtttt ataatagttg
     5641 tcatctgcac cttcagaaac catcctgagg agaaaataat ttaaggcaac ctcaacatgt
     5701 tcaaaaggtt caaatgcttc gtataattgc agaaagggtt tgatacgttt tttatcgaaa
     5761 ggttcatttt gattttgagt tgctgttata taattgtgaa aagtaataaa tactttaagc
     5821 aacaataatt cggtattagt tctgaaagaa gtatcattga tcataaattg agaggcatga
     5881 tgtatgcgac ttctgttcat gcggatattc agaagtgtat gtaaatctgc taaccaaacc
     5941 tcagttctca atcgctcacc gatatctatg ctatcggttt tcaaatagct gcttattttc
     6001 tttaaatttt tttctaccag cgccaattgt ccacgttgaa ggagctccat atttagataa
     6061 aatccttttt gtgagcttcc tgaggcaatt actaattgtg tgatgaatga ttgtaataac
     6121 tcaagaaaat cggagcatat atttctatag ccggcatcat tccaagcttt ggactgggtg
     6181 gtttcgatat aaatgcgctg cttttccggc tcagagatga caaaacccgg ttggtatggg
     6241 ttgatttgat gataaaacct tatgaacttc tcatttttat taaaaaaagg tgatgacaaa
     6301 aaatccccga actcacgcca ctcgctcttg gtgagggttt gtaacaactg ctgcaaattc
     6361 gtgttttcca caccttaaag atgccaaatc tgctacaaat gaaaaaattt ctactggtat
     6421 ggccctgtgg aataaagtca ctttgcagca acatcagcta tatcagcatg caaaacatgt
     6481 gatgacagct gaaaacatgg aaatcagaaa cttaaaaaac attatcatga aaaaacaatt
     6541 atttacttca atctttactt tgtcggcatt gtttggcgta gcccatgcac agacaaacac
     6601 attcccaacc acaggaagtg ccggtatcgg aaccattact cctgcctcga gtgctatcct
     6661 tgacatccaa agcaccacac agggtatgtt ggcacctcgc atgacaaagg ctcagcgtga
     6721 tgctatcgtt tctcctacta cgggattatt gatataccag acgaacagca gtcccggatt
     6781 ctattattac accggcgcta cctggtcggc aatggcagca aaaggtgcca ataccagcct
     6841 ttccaatttg gcggcatcta ccagtatcaa tacagcatta acacccaatg ctaacaatac
     6901 cctcgacctc ggttcaacca ctaacaactg gaatgaactg tatgtcaata ccattaaatt
     6961 catggatggc accacacaaa ttactgcagg tggcggaggc ggtgggggag taggcggaag
     7021 tggtactata agctatcttc caaaatttac ttccgctaca acgctcggaa attctactat
     7081 ttacgataat ggcggaaacg ttggtatcaa tactaccact atggtgggct cggcgaactt
     7141 cgtttcaaaa tctaactccg ttaccggata tggcggtatg tatgttgaaa tgggcggcac
     7201 cagcggaaga aaaccgtttt atggttactc actaagtggc attgcaaaag catggacata
     7261 ttttgatgag gcgaccaatc aataccgcgt caacaataat ggcgacagaa tggttatcga
     7321 taattcaggt aatgtaggta tcggaacaac aactccaagc aatttattac atgtcgaaac
     7381 atccggaact gttactcctg taaaatttgt aaagccatgg acaggaacag gtaccactga
     7441 tttctttttg ttggaggtga gtaataatta caacttcggt tacggtagcg ggatcaatgc
     7501 gtatggtggt aaaatgggta ttaaaggatc tgcatttaat ggcgatcaga ttggctatgg
     7561 tgtttatgga tatggattcg gtggcacagg tacaacctat ggggtatatg gcactgccgg
     7621 ctccagcggt tcagccatcg gtgtttatgg cactaccaca agcggatcgt cgcaatgggc
     7681 cggttacttt gttggccgtg gatatttttc cgataatctg ggcattggaa caagtacgcc
     7741 ttcaacaaaa ttacatgtga taggaggtac ggatgtagaa ctcgcaacag gaggatttgc
     7801 tacattcgga gccaccactg caactaatat agctatcgac aataatgaaa tcatggctcg
     7861 caacaatggt gcaacttcta ctttatatct caacaatgat ggtggcaacg taagtatgtg
     7921 ttatgcaagt ggttcagtta tgattggcgc aagtgtacct gcaacgggat acttgttgag
     7981 tgtggatggc aaagtgatgt gcgaagagtt gaaagtgcaa atgagcgaaa gctggcctga
     8041 ttatgttttt gccgataatt ataaaatgcc gacactctac gaattggaag catcaattaa
     8101 agcaaataaa catcttccgg gcattccttc agcagcagaa gttgcagaaa atggcatcag
     8161 tgttggcgaa atgcaaacaa atatgatgga gaagatcgaa gaacttacct tatatattat
     8221 cgaattgcag aagcagatcg atgagctgaa acaacaataa tttcatcgat atcaacttac
     8281 gatcatgaaa aaattattga tcattagcgg tattgcatgg atgtgtagta tcgtcaatgt
     8341 aaaggcgcag agcatggcat tgcctttaca gataacaaat aaatatcaac tgagtattga
     8401 acccggtagc actcgtgaat ggatttcttt tcgtcgcgat gcaccttttc aggcacttac
     8461 catttttgaa caagcacctg atctattggg attgcgcgaa caggatgaat ttcgcaaatt
     8521 ttctgaacgc accgatgctg ccggaaataa gcactataag tttgcccaat atgttaacgg
     8581 tgttcgcgtt gaaggaattg aatattatac gcatgaacga aatggaagat ttcatttgat
     8641 gaatggcgat gtggtgaaca atttacagtt gtcatccacc ccgtcaatca gcaaagagca
     8701 ggcaattgca agtgcattag ctcaatttcc tgcaactaaa tatatgtggg aagacccgag
     8761 agcagaagct gcatttaagc aaaaaaacaa taatccgaat gcatcattgt atcctgatcc
     8821 ggaattgttg tttgtgaaaa tagatccaaa aggatcgcag gttgccgaca attatatttt
     8881 ggcatatcgc atgtatgttt ttgctgttga accgcatatt gctgaatatg tatatattga
     8941 tgcaaccaca ggtgcttttc tgcgcaatcg gtcgttggaa attagttgta atccatcgtc
     9001 aatggctaca actttcaatg gcacgcaaac cgtaaataca gattacagga ctgaagattg
     9061 cggatctacc tatactacgg aaaccagcta tttttctatc gatgattgca cgccgggttc
     9121 ggagataagg agctatttca gtgcatcgta tggtgcatgg aattatggcg atgattggct
     9181 gttatgtgat aatgataatt cttggacaaa tacaggttct tacctgatgg tgatgacttc
     9241 attgtggagt actagaaagg caatgaatta ttatttaaat gtgcatggtc acgaaagctt
     9301 tgatggatcg ggaggcctga tagatctttt tagtaataaa acatatttcg atgatgatga
     9361 tctcccttat tgccgaaatg ctaattacac caatattatc gacaatctga attttggttc
     9421 gggaaatgat tgtatgccgg gaaccatgga tgactataat acattggata ttgttggcca
     9481 tgagttcaca catggtgtca tagaatatgc gcactttgat gcattggatt attccgatga
     9541 aagcggtgct ttgaatgaat catttgccga cattttcgga gagatggtgg agtattatac
     9601 cgaaggcggt gataccctta gttggctttg tggtgaagat aggggtgcca tcagatcttt
     9661 taaaaatcca aaagcttttg gcgaccctga tacatattat ggcactaatt gggcaacatt
     9721 aggaggcact gataatggcg gagtgcatac caatagcagt gtgcaaaatc atatgtttta
     9781 tttgctcagt cagggtggtt caggtgttaa tgaccatgga attgagtatc aggtagaggg
     9841 catcggttat gatcatgcaa gagatattgc ctgggttgcg atgatggatt atctcgatgg
     9901 ttctgatgga tacatcattg cacgtaatgc atggatacaa gctgcaatcg atttgtttgg
     9961 ttcatgttct caagaggtca tctcagttgg acaagcattt caggcagcgg gagttacaga
    10021 gtatacggcc tttgataggg catccgtttg cggaacctat gtgctgagtg gttatgcaga
    10081 tgcaacttat ggcattgaaa attcaaccat ggtgcttggc gatttcctaa ttgattgcac
    10141 tacaactatt aatgcaacag ccgcagttac attcaagtcg ggatattata ttcagttaaa
    10201 tcccggattt acggcattga gcggatgtaa tcttaccgca tatatcagtg catgcgaaat
    10261 cagtgagtac gatcctgata atgtgcgtat tgcgcaatca aacttgcagt ctcccacagt
    10321 tgccgaaatg gatgataaat tactggtgtt tccaaatccg gtttttgaca tcgtaacgat
    10381 caactgggag cgtgaaattc cgatggagca actgcaaatg gtgaatattg ccggtcaaaa
    10441 tgttacagat catatctcca tcgaagagaa tacattggga aaggcaatta ttgatatgcg
    10501 aaacctggcc gcaggtatgt atatgattgt catcaaaaca gccgatggcg aggcatatgg
    10561 aaattgtatc aaacaataaa tgttggctgt aagtacacaa aaaagccgac aaataatcgt
    10621 cggctttttt gttatttcat ttggcataat taaatcccca aacactgcaa cataaatgca
    10681 tacataaagg ctacttcatt aacgctttcg aaccttccgg atcgaccgcc gtgacctgca
    10741 tccatgttta cttttaatag caacatgttt ttgtctgttt tggtatagcg caattttgcc
    10801 acccactttg tcggctcaaa atattgtacc tgcgaatcgt tcaatcctgt ggtgactaat
    10861 atgttcggat aattctgagc agttacatta tcatatggag agtaactcat catataatca
    10921 tattgctcct tgatttttgg gtttccccat tccagccatt caaatgtagt gagtggaatg
    10981 ctttcgtctt ccatggtggt gatcacatca acaaatggaa catcagcaat tacgccttta
    11041 taggtggttg gtgccatgtt taatattgca cccattaata atccaccggc actaccgcca
    11101 ttggcaaaca atttatctga ggccacataa ttattttttt gcaaccagat actgcaatcg
    11161 atgaaatcgt agaaagtatt ctttttattc atcatcttgc catcatcata ccattcgcca
    11221 cccatttcct gaccacctcg aatatgtgta ttcgcataca caaatcctct atccaacaaa
    11281 ctcaatatgt tggcgtcaaa ataatcttca ttgctatatc catatgagcc gtaaccataa
    11341 atgaatccgg gattggtgcc atttttatga aacatatctg tacgatagct aatagtgagt
    11401 ggcacctgct tgccatcgcg ggcggtaacc ataatgcgct cagttttgta taaatctttt
    11461 ttatagccac tcggaatttc ctgctgtttc aatatttctg tcagcgatga agaaacatca
    11521 tagtgataag tggtaaatgg tgtgatgagc gaggaataat gatatctgaa tccggtagca
    11581 tgataatccg gattgtagcc aagcgttgca taataggcag gctcgtcgaa tttaatcggc
    11641 acatctgttt tatcattcac aataacacgt agcattgtca atccgttttg cacttcttct
    11701 gctaccatga aattgctgaa gtattgcact tgagttaaaa atacatcatc tcttccgggt
    11761 ataacatcct gccaattatc catgcgcgga tcgaaataat tcacgctaca taggcgatag
    11821 tttttagcct tgtagtttgt gagaatagta aattcttcac ctttaacatg atcgacattg
    11881 aatacaacgt tttctaccaa tggcgcaact tgtttaaatt gacgaggatc acgtgcgtcg
    11941 gcaaaccaaa cggtatttgc atctgtgcgt cctgaatagg cgaaaataaa atcttcactg
    12001 cggctgccgt aaacatagat gtcgagcgta ggatcttttt cttcgaaaag caatttgtca
    12061 tctgaagctt tcattcccag gaaatgccgc tttacctgat acacacgcag ggtaacggta
    12121 tccggaacac catatacgaa tgagttatta tctcggctcc atacacatcc atttcttgtg
    12181 ccgggcacct cgtcgcttaa ccattgcttt gtttgcagat ctcgaacgcg aatggtgtaa
    12241 aaattgcgac catgcacatc cataataatg catgctttgg tttcatcggg actgacttca
    12301 acttcgtagc tcaggaaatt atgccctttc gccagttcgt ttccatcaac cagcacttcc
    12361 tcctttgcat ccagagttcc cttcttacgg cagaagatcg gatattctcc tcctttttca
    12421 tatctgtcat agtaccagta attacctttt ttataaggaa cagaattgtc atcttcctta
    12481 atccgaccta ccatttcttt gaaaagctgc tcctgaatct ttttatatgg agcagtaaca
    12541 gcttctgtat aggcattttc agccttcagg taatcgatta cttttggatt gtcacggtcg
    12601 ctgatccagt aataaggatc ttcacgatcg ccaaagctgc catggatagt atgctgaatg
    12661 cgttcggctt ttggtggtat ggcctgttgg gcattcagtt gacccgctga cgctactaag
    12721 aacagcaagg ccgggagtaa acactttttc atggctgaaa attacaaatc cctctgatat
    12781 tgtgacagtt accctgtcac aaatcgcctg tttttttaca taaatgttat ctattgttaa
    12841 agtgaaatgc cttactttag cgacggacaa aaccgacaga tatgcagaaa tttctattcc
    12901 tggctttatt ttcacttgcc ggcatgcagc tattcggaca gggtcgtacc attaccggac
    12961 gtatcaccga cgcagacaac ggggtatacc tggcaggggt tacggtctta ctggaaggga
    13021 ccatgaacgg aaccgtctct gatgcagagg gtaaatattc cattacagtg cctgaagagg
    13081 gaggtaacct tatcttctcg tttatcgggt atgagaacca aaagatcaca ttaggtacta
    13141 aaaacgtatt atatgtgtct atggccatgt cacaagagat ccttgatgag gtcgttgttg
    13201 tgggttatgg tacggagatc aaaaccaagg tcacaggaag tatttccgag gttggcaatg
    13261 atgaaatcgc cctaacaccg gtttctacgg ttgagcagac acttcaaggt agagctgcag
    13321 gggtattcat cgaatccaat aacggaaaag taggaagtga tgttcgcatc cgtatccgtg
    13381 gtaactcgtc tattaatgcc agcaaccaac cattggtggt tgtagatggt gtgccaatca
    13441 atacggtagg tgtcaacgac tattttaacg tggtattgaa ccctatgaac gacattgatt
    13501 ttaacgacat cgaaagtgta gagatattaa aagatgcctc tgctgctgcc atctatggct
    13561 cgcgaggtgc aaatggtgta atacttatca ccacaaaaag aggaaaagtt gggaagccga
    13621 ccgttacact cgattatcaa tatggtttca gcaaaccaac gcgcttgcgt gaattcttaa
    13681 atgcacaaga atacatcgac tattatacac aagctgccat caatgccggt gaatatgatt
    13741 tcaccaatgg attaagtggt tataccagcg ttgaagaggc tattgacgat tatatagctg
    13801 acgcagaaaa aaaattcaat aagctgcaag gttataccga ctggcaaaat gattatgcca
    13861 ataccgactg gcagtcgctc gcattccagg aagcaacttc aaccatgttc gacttttcag
    13921 tttccggtgg ttctgagaaa atgcaatatt ttacttctgt aggctataac tatcaggatg
    13981 gtattatgat cggcaaccac ggcgatcgtt ttaatatcac caataatatg gatgcacagc
    14041 ttactgaaaa gctgaaactc ggcaccagcc ttacaatcag ccgttctatc aacaacgata
    14101 ttccggatga taattcattc tctacgccat tacaaatggt tgcgcaatca ccattgactc
    14161 caccgcagga tactacaggt gaatattata atacccctac tacgttatat tataatccgt
    14221 tgatagaggt agataatgta tttttcgaaa gtactacttt ccgcaccatc ggcaacttaa
    14281 gtgctgaata tcaattacgt ccggtattgg cattgcgcgc agaattaggt ggcgatatta
    14341 ccaatcactg gcaacaacgt ttttacggaa gtaaatcgga agtaggattg tctgtaggtg
    14401 gttatgctac caacttcaac tctaccgttc aatatttcga taccaagatg ttgttattat
    14461 acagccagaa atttggaaag agcactttag gtgcaacaac aggtgttgaa tacaatcagt
    14521 atgacgatca atataattct gtagaaggac aaggattccc ttcagataaa ttgcagacac
    14581 ttgcaagcgc tgcagaaatt gtttacggaa ctggtgttct gaatgcatat cgcttcttat
    14641 catatttcac tcgtatcact tacgattacg atactaaata tctctttaat gttaccgttc
    14701 gtgccgatgg ttcttcacga tttggagaaa ataaacgcta tggtgttttc ccatcaggat
    14761 ctttcggatg ggtagtttcc aatgaagatt tcctcaaagg aaataaaaca ctcagcttcc
    14821 tgaaggtacg catgagctat ggtatgacag gaaatgcagg tatcggaaac tatgattaca
    14881 aaggactcta tgccgttggt tcttacgcag gaacttcagc attgtatcca tcaacaattg
    14941 ccaatcctga tcttacctgg gaaaaaacaa ctcaggcaga cttaggtatc gatttcggat
    15001 ttatcaatga tcgcattaat ggcgaatttg atgtgtatct gaagcaaact accgacctct
    15061 tgcttgatgt gcctgttcct tcaacaacag gtttcactgt gcaaacacaa aacgttggta
    15121 gcctcgaaaa taagggcgtt gaatttacca tcaactccga caacttcgta cgcacattta
    15181 catggagcac cagcgtgaat ttctctgcca acagaaataa aatcactgaa ctggcaaatg
    15241 gtcaaaccat catcgattac ggtggatcag atttcatgaa tgtggtaatg gtaggccagc
    15301 cactcggcgt tttctatggt gctgaatatg caggtgttga ttctgaaaat ggagatggtc
    15361 tttggtatgt aaacgatggc actgatgcta caactaatga tttcaacgat gcgaattttg
    15421 ttgtagttgg tgatccaaac cctgatttca ttgcaggtct taccaataat ttcggttata
    15481 agaacttaag cctggatgtt accttgcaag gcgtttatgg taacgatgtg catatgtcgg
    15541 gcgactattg gatgtcgtca aatgccagcc agtgggataa ccaaactgtt gatcagttaa
    15601 atgcatggca gaatcccggc gatgttacgg atgtgccaga aaacagatta ttatttgaaa
    15661 atggtacagc tgcacgctcc agccgttatc tcgatgatgc cagttatttg cgtgtgaaaa
    15721 ctgtaacact gagttacgac atgcctaagc gtgttaccgg taaattaaat atgagtgcaa
    15781 tgcgtttgta tgcttcagca tataacttgt tcaccgtaac aaattaccgc ggttgggatc
    15841 cggaagtatc cagcgattca tttgtagata atatctatta tggtatcgac ttctatagcg
    15901 ctccgcaacc acgcactgtt gttttcggta tttcacttgg tttataatgc atgtcaaaca
    15961 aatgaaactg acaattatga aaaagaatat tctatataca ctttgtgcaa taatactcat
    16021 aggcggttta aactcctgtg aagatattct tgtattggag cctgctcaaa gtttaagcaa
    16081 tgaaactgca ctggacaatg atctcggtgt aaaacaaaca cttgttggtg cctatgatcg
    16141 catggcgcaa tcatcactgt tgggtggtga tgtaatcatg aatgcagatt tgtattccgg
    16201 tgccggcgaa tttgaatggg taggaaccta tgtcgattat cgcgaaatat ttacacatga
    16261 tatcctggta aataatggat ctgtaagctc actttgggac gatgcttatg aagtgattaa
    16321 tacctgcaac aacgtgatca acgctattga tgtagttgaa gcggcagatc aggatcgcgt
    16381 gcgtggtgaa gcactcaccc ttcgcgcctg gtgttatttt gaattagttc gggcgttcgg
    16441 atatcaatat cagccgggtg tggaaaatac gcaattaggt gtgccactga ttcttacgcc
    16501 tacactcaat tttggcgata acacaaatgt taccagaaat actgtggaag aatgttacgg
    16561 acaaatcatc ggtgaccttg agcaagctgc aacattacta ccggagaaaa atgatgtata
    16621 tgtaaatacc tatactgcat ctgctttatt ggcccgcgta tatcttcaga aagaggacta
    16681 tgagaatgcg cgtgatgctg cagatagagt gatagcttca aatgattatg aattgatgtc
    16741 aaatgttgca gatgtattca atcaggatga tgcaacagat gaagatgtat tcagcgtgga
    16801 gatttcgacc actgatggca ataattcatt gaataattat tatgccacca atgatttcgg
    16861 tggacgcgga gatatcgaaa ttcaggatgc acatatggcg ttatatgatc cgctagatgc
    16921 acgtgctact ttgtttttta ttgatggtgg cattcgcttt acttcaaaat tcaataatga
    16981 attcggaaat atttcagcta tcagaattgc tgaaatgtat ctgatccgcg ctgaatgtaa
    17041 tcagcgtttg ggtacaagcg taggtgctac acctgaagat gactataacg ctattcacac
    17101 acgcgcagga ttgccggcag cagcctctgt ttcgctcgac gatatcttat acgagcgcca
    17161 cttggaactc tgctttgaag ggcaggccat acatgatatt aaacgcctcc atggtatggt
    17221 gggcaccttg aactacaacg atcctaaatt gatatatcct atccctcaaa cagaaattga
    17281 ccgcaatgcc ttgttggttc aaaatgatgg atattgagca agacaaagaa acaacacaag
    17341 gccgggatag cgatattccg gccttttttt gtcgattaac ataaatttgt caaaaatgga
    17401 ccatttgtga catgacaaat gaatagaata tttccaaaac tatgcctccc ggacctcagc
    17461 agtgagtttt tgtgataaaa tgtgacatga tttaaaatgt cacattttat cgataattag
    17521 aaccactaag gcattatttg taaatttata aaccaactca caaagggtcg gtattttttt
    17581 cacaaataaa accttcaccg atgaaaaaac tgttgatgcc cgtgctcctc gcggcagggt
    17641 tagtattcgc aacagccggt tatagccagg atgctgtcaa gttcgatcag cacttccagg
    17701 ttcaattgac cgacgctatg ttgcaatcag gatctgtagt tatggatatt tccgacctgc
    17761 agttcacaga cgaagctgcc gctcagaagt acttcaatgc tattagaaac aaccttgttg
    17821 agtttacact tgactacagt gcgcacacgg ttactatgca tttgtatcct gaacgtctgg
    17881 gcaagcacac ttggacatta agcgattgga atgcttacct ggcgaatatg gctgaacgca
    17941 atgcagaaac ctatcagtca tttcaaattt catcacctaa acaataaaac gcatgaaaaa
    18001 gaaaattcta caaatactac ctgcggcatt attgatcaca ggtgttatgc aaaccggaca
    18061 tgctcaaatt gtgagcggtg tttctttcct taaagggaaa tggatagaac tggcagtttc
    18121 tgattgcggt acttatgcat ccggtggtgc aggtatgata gttgcagctc ctgccactta
    18181 tcatgaaaat acaattgatg gttcattgag ctttggcaac gacgttacag ccgatggttg
    18241 ggacattggt gttcctgatc agtgcggcga tttcatcatg ccgggttctc ctgaagaggg
    18301 ttttgccatt cagattggta caggccctgt atacggaaac ttgcagccat attgttatcg
    18361 cttcggtgca ttcgtggccg cagcacctga ttttgttgga acaaacctta ctaacgtaaa
    18421 tggtggttct atcagaaaaa cgttgtggca aggaacaaat acaggtatcg gacttgctgt
    18481 ttctcagaca acatattggt tgaatagcaa acaatcttat atcactatcg ttgacctttg
    18541 taatggtggc gatgatatgt ttgatgtgta ttatgctcgt aatgccgatc ctgataacga
    18601 tcagattact agcggtacat tcaacaccaa taacaacgcg aaaaaacaat atgctactgc
    18661 cggttattct caggtaactg caaccagtac agttggttct ccttgttata tggcctatgt
    18721 aagtaacgac tctcgcgcaa aagtttctcg cggtaacttc tctatgggtt caccttctga
    18781 tatctggaat ggtcttagcg gctatatcca aactccgggt gccgttaacg cagatgctgc
    18841 cattcaaatc tctttcaaaa ttgatacact gttgcataat gattgcggat gcttcactta
    18901 ttcaacaatg ttgacatcag caggtcttac agatcagctt gcattgacag gaactgcttg
    18961 tgcaactctt ggtacacttg ctcgttacgg tgaagacatg atagatggtt atttgaatga
    19021 tccaactaat ttccttaaaa atgaaatggt ggtttatcca agcccgtcaa atggtaattt
    19081 cactgtgaat attttcgaaa tggaacatgc aaatatcact gtagtaaatg ctgttggtca
    19141 ggttgtttat caagttaatg atgtaagcaa aattgcgggc atcacacttg ataatcctgt
    19201 tccgggaatg tattttgtaa atgcagaata cggtgaaggc aaaaaaatca ctcgctcgct
    19261 tgtgattgaa taatgtaagc gattttacat catcataaga caggatccga tttgggtcct
    19321 gttttttttt atttgaaaat tagcaaatgg gatggtgcat gcatggagat tatttttggt
    19381 gagcaagttt gagggttagc aagtgagggt aattagctaa tttgggaatt agcaaattag
    19441 caagtttgca agttagcaag tgtggagtgc ttttaacgct ggcgcgttaa tagggaatgc
    19501 aattatattg ctttg
//
LOCUS       DAOQOU010000043        19382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1409_length_19382_cov_6.192632, whole genome shotgun sequence.
ACCESSION   DAOQOU010000043 DAOQOU010000000
VERSION     DAOQOU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19382
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1409_length_19382_cov_6.192632"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PLM90_05660"
     CDS             complement(<1..1170)
                     /locus_tag="PLM90_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88889.1"
                     /translation="MKKLLLILCLLLLAGDLFTQTNTFPATGNVGIGTTTPTQLLQVN
                     GGIAQFGGTTDYSRFLADGDLTFTGNADYLVGGDRFAFRYQGNPNFGLVFSATNSRYE
                     FRDGTGTAVFWQGGNGNAYIKGFLAIGSTATPNAALDITSTNAAAIKINPYGTAAGNT
                     GGLRFLELAANGVNYVGFKSPDNIVNNKIWVLPATDGTAGQYLKTDGAGNLGWASDAG
                     TAYSAGTGISIAGTTISNNLPDQIVSLTGTGATTVSGTYPNFTINSTDNNTIYSSGTG
                     IDVTGTVITNTLPDLPVTLTGDGATTISGTYPDFIISSTDENTTYAAGLGIDVTGAVI
                     TNTSPDQPVFLIGEGATTITGTYPNFTVSSTDENTTYSAGTGILITDTVISNTGDV"
     gene            1744..2484
                     /locus_tag="PLM90_05665"
     CDS             1744..2484
                     /locus_tag="PLM90_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88890.1"
                     /translation="MQTTTHTTSRSNQGLVILGLVMSVSLFFSCAAKSKEEPVLDGRV
                     FTVLTIPDGNSAEAAMERISFTNGKFDNDNCHLWGFGDGAYAAKEAGDSITFTATTTS
                     AKEGTMTWTGVIKGATLMGTMVWSKEGQSDLNYHFTSANIEEVDLNGISFAVQFSDGD
                     TAQAETITFNNGMFESPGCYTYGFSASPYQAYMLDGKQHFQCLYTSAKEGKMLFYGVI
                     DGTSISGTQFWTKEGQDDVYYKMSGQKL"
     gene            complement(2541..4232)
                     /locus_tag="PLM90_05670"
     CDS             complement(2541..4232)
                     /locus_tag="PLM90_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ88891.1"
                     /translation="MKNQITKSIIAICMMLCTQWLPAQTVEISRPEKIIGCEEVPASL
                     LAPNGKLAHANNPMYYNSTTTGNKMDLTPKLDWPLKMSDEYKDLSGVYSYFYISNYLD
                     LDPDTTVKEDWYCFTNDYARNYEGHNGADICPYPYGWQMMDDESVDVIAAADGVIGAI
                     FDGNTFDRNCSKPHTFIINDTLDNTGYLGNYVRIDHAGGLTTLYGHLKHGTLTDLNVG
                     DSVKSGDYLGKIGSSGNSTGPHLHFQVMWYGSLVEPWKGNEDVGEPNCNFTYYQSLWN
                     NQIPYYNTQLIRVATHFGAPTETSCTNYEAGSNETETLVNHFSSFQIAKIGVSIRNIF
                     AGMPINVDIFTSTGVLKNSMSYTPLVFDEIATYYFDQSLLSYTTGTYRVRVTFDGKTY
                     DHYFTVNCSGDLIYSAAQSGHKGFISGGSITSTSTISGTSANEILYEAETFVKLNVGF
                     QAAMNCEFKARLDDCTLGGVKEEPEPIKSSDSRLTVYPNPTFGEFSLLYEGTSENDAL
                     VNIFDLTGRLITQYTFSSNQGNVNHTFDLSEQAKGLYFIEVRQGDNVSTSQLIIQ"
     gene            complement(4258..6321)
                     /locus_tag="PLM90_05675"
     CDS             complement(4258..6321)
                     /locus_tag="PLM90_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88892.1"
                     /translation="MKKQITLFAVCFIAGLLTVNAQTNTFPATGSVGIGTITPNASAL
                     LEMSSTSKGLLAPRMTKSQRDAIASPATGLLIFQTNSSPGFYYYTGASWTAIASKGAN
                     TTLSNLTTTSISQPLLPNADNTVDLGSSTLRWNELYVNNIKFLDGTTQTTAGGGGATY
                     TAGTGISIAGSVISNTGDVNAADDLTILTNHGGDVTGVYNNLQISTGAVGSAEIADGS
                     VTSTDILNGTIAAADLSSMGATSGQVMQWNGIAWIATTPAAGAETDPQVGTISTNAIP
                     RWDGTALTTGGIVDDGDNLSFGWNNGISSNTTASFYNNEGVIADEDIAIKGVDQTVSL
                     GGTATVHSTGYLGYNTVGVVFGDTPVSHVGVWGNASTTTNSAAVYANNSSTGSDNYGV
                     VAKSVGAGTTNIGLYARSAGATNNYGVIVPTDGGKSGFNWSSPSALVGIKGNDTDNAL
                     RIIGTDFNTDLIVDEVGRVGVHGDPDAFSANFNVYGSGYVQDRLNIGVSGAFLYSMLA
                     VNGTDETVTIMGTNPYIQMKNAGSNVGYLRANGSDLMLATNSGVTGKVILRANGINSM
                     YVDQNGNIVMGSTTVAPKSGYKLSVDGKVVCEELLVQLSPWPDYVFADDYKLSSLSEV
                     ENFINENNRLPGIPSAAEVESEGLNVGQMQKLMMEKIEELTLYMIELQKQNADLQAKV
                     DALSN"
     gene            complement(6408..8309)
                     /locus_tag="PLM90_05680"
     CDS             complement(6408..8309)
                     /locus_tag="PLM90_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ88893.1"
                     /translation="MTRWTAHIYIFLLSICWINNISAQQDIDSLRKVIKNTKDEETIA
                     DTYNDLAYQFAYSKPDSAIFYGNMALDLSEKHNLQEQIAPAYNAIGLAYQSKGMYQIS
                     IDHLTKQLPYLKSDSSALSRTYHNIGLSYKFLGNLNTALEYEIKSAKAIEKSSDPVAL
                     GAIYLSIGNIYRDLQDYDLAYSYLQKSLKTYESGTNFGISSKNSLIAMTYSGIGSLFL
                     VKYEIDSAIYYHSKAIDLLEKEPVSYNLAILIENLGDDYLVIDEREKAIKQFQRAKQI
                     MEALQSGADVGYEYYKIANVYRIAKQYNKALSYNDSALVLFTKYDAVNFKLASYDLNF
                     RIYDSMGRPAEALENYKLYIALKDSLNSESQQSEVLRLKEEFETTQKEQRIELLETSN
                     ALQLRENERQVMFRNIAIGLIIMLLGFGLLIWNRYRIKQQVKQLQMRNDIAIDLHDEV
                     GSSLSSIKMLSEIAALQKDDKHQLDDLLGRIQVNASETAEKMHDIIWMINPKHDQLSG
                     ILSRMENFMFDMCSPGEISFNLQKNIDPNIKLNMLQRKDLMLIFKEAVNNAVKYAAAT
                     NIQAHISYVDHHVQLIVTDNGKGFDLQTIRRGNGLDSMQMRAQELAGTLEINSTPNTG
                     TTIRVRFPI"
     gene            complement(8333..8971)
                     /locus_tag="PLM90_05685"
     CDS             complement(8333..8971)
                     /locus_tag="PLM90_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ88894.1"
                     /translation="MDIRVVLFDDNKHIRESLQMLISATPGITCIGTYPDCRDMLIYI
                     ESDVPDVVLMDIEMPGMNGIEAVKLIKQHFPGVQILMQTMFDDDDYIFEAICSGASGY
                     ILKTTTPLGYTEAIKNVHAGGSPMSPSIARKVFELFKTNLASQVDVNYNLTDKEKEIL
                     QNLVKGKSYKMIAEEMSISTETVKTHMKNIYTKLHVNSNTEAVVKAIKDKII"
     gene            complement(9164..9619)
                     /locus_tag="PLM90_05690"
     CDS             complement(9164..9619)
                     /locus_tag="PLM90_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88895.1"
                     /translation="MQTNQQLSWYKKATIILAITTIIPTTIVLTSQAAEPLPAQQVVT
                     SQPISTEDAARFVANYADQAEKVKAVVKGVVIEAGQLESLNTVAAAHPNANKFRLYFG
                     KDDNGGDVTIAVAMDGQGNDQTDTMYSSARTGTNLCPPVCDANGTIGGN"
     gene            9716..11548
                     /locus_tag="PLM90_05695"
     CDS             9716..11548
                     /locus_tag="PLM90_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88896.1"
                     /translation="MHTKLSGFFTWIVFTIVPFIGFAETEPGPNNNAVTYNLPVIDKS
                     HIESDLNHATETLQRISLLGELAAYYVTWEADAHKADSLLSVGIDMAELSYNNEFLLE
                     AYSNYLIYIDDYTFTDKVSRIVPLLESMSGQLTNKKEVWKCKYALAYGCKLIFQTDRA
                     KDYAHQALTEAIRLKDNNLTAESHLLLGSLQHDMNNNVEAIRNYLDALTIAENGSDLE
                     LRLKCYDELSTFYNLIKAYDKSINYKLKELNIAEHAKKPDSMRIMTIKFDMEVIAFNN
                     RTLNENQLYKIIDYSNRNGVSRLKRFALVVFRNHLIKQSNFAELYHLYNEEYPEELEY
                     IRTNDTTTYFRLEAMFNEYEGDVVTAKEYFDKAAQGINRSKDKLRQSSFYLRYGDFLQ
                     RNNFLEEARDCFRRSYTIAASINYYEFMVEATGKLEKIYSLQNNFEEAYAYSLLNKST
                     SESLDSLMQKEQLQILELENEEVLRDQQLQQERDETRRRNNIQYTAITILIATFFLML
                     FILGGIRVSPAIIRFLGFVSFIFFFEFLILLFDTWIHHLTHGEPWKILAIKILLMCGL
                     VPLHHMIEKRVISYIISHKMNLIPKRSPKPVLVEADDVREEEKA"
     gene            11724..12743
                     /locus_tag="PLM90_05700"
     CDS             11724..12743
                     /locus_tag="PLM90_05700"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMZ88897.1"
                     /translation="MSFAQTFAVDLENPISTSFSFLSSTPNAGVNAMGEAGTAVINNG
                     ALYTNAAAISALLDSFGLALDYKRGAGNLLCLSVYNQYTSSALAFGLRYNSLGGSTLI
                     TPTGNINQGASNYELAFDVAGSHSFSPHFMAAITGKVFYSTISKDMIINGVVIKPSSG
                     IAVDISCLYTTTVNGTDKINIGATISNIGSPQEYGYPGYDYFIPTTLSIGFAYDVLFD
                     DNNQLVFALDVNKLIVPTIYNRSESAAYGMLHSFNDAPGGVQEEMHEITLGAGIDYSY
                     QEKVHLRAGYFYEHPSKGGRTYFSLGGGIAIGNGNLDVAYRFYDNTQAIVVANALSLT
                     LSVNL"
     gene            complement(12740..13336)
                     /locus_tag="PLM90_05705"
     CDS             complement(12740..13336)
                     /locus_tag="PLM90_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88898.1"
                     /translation="MKIRNLLLLCSIVVTFDCSAQFISLRATNASQFISDGLYDPDIS
                     KAIGAELIYSKPVNFIKWPINYNIGIDYRNVDGAHAAYVATGLTWVTRTPSGFMFAPN
                     AESVTYVTSNWLHYADVNMYNGVLIGDSTYTYSMGWSLGYNIGYVFGKQFFVHYGFAG
                     RYNTTPQYHNDAIEGASSWDFMLKAGILWKFKRKFYKA"
     gene            complement(13358..13978)
                     /locus_tag="PLM90_05710"
     CDS             complement(13358..13978)
                     /locus_tag="PLM90_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMZ88899.1"
                     /translation="MKTCPHCHNENPVDFSYCRYCGNALNGVPENKPKSIWKRLPAWV
                     WIILIVGGIAALILTLIGSFIAIATMEGVASIVILICGIVGFGVIPLRRPDRAGSFAR
                     AIGLSFFALMGATVDQTGNFLYNKPIEMCFCHDGTSLTRDIDVTNPIPGQTNITQDYT
                     CYDSTGKAAKTINIFAVLGIRFVEYVILGYLLIALRQVRWRIKNNR"
     gene            14033..14356
                     /locus_tag="PLM90_05715"
     CDS             14033..14356
                     /locus_tag="PLM90_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88900.1"
                     /translation="MEPLSNQQDQQQQHNPNLFWEGFFYLSFFVFFDIVVIPYAPKSI
                     TFDQYYPLAMVIVGGAGLFVGMLAFRIMKNWPNFIKAGLFVVMYGIILWYLIENINKF
                     TVPWM"
     gene            14506..15792
                     /locus_tag="PLM90_05720"
     CDS             14506..15792
                     /locus_tag="PLM90_05720"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HMZ88901.1"
                     /translation="MSETAHIKLGEQEIDLPVFTGSEGEQSIDINKLRDLTGYVTLDS
                     GFKNTGATKSSITFLDGELGILRYRGYPIEQLAEKSNFMEVTYLLLYGELPTNEQYIE
                     FTDRITRHTLVHEDIKQFFDGFPSNAHPMGQLCSLMCTLSSFYPDALNPHMSANEVDM
                     TITRILAKMPTLVSWIHKKSVGHPIMYPQNKFDYVTNFLYMVFGMRTEEYMPDPVVVD
                     ALDKLLILHADHEQNCSTSTVRMVGSSQANAYASVAAGVAALWGPLHGGANQQVIEML
                     EVIKRDGGNVAKYVEKAKDKNDNFRLMGFGHRVYKNFDPRAKIIKEACDNVLNKLGIH
                     DPVLDIAKQLEEVALRDNYFVERKLYPNVDFYSGIIYRAIGFPTEMFTVLFALGRLPG
                     WLSQWKEMRESGEPIGRPRQIYTGPTQRDYVNIAIR"
     gene            complement(15873..16322)
                     /locus_tag="PLM90_05725"
     CDS             complement(15873..16322)
                     /locus_tag="PLM90_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88902.1"
                     /translation="MHQPKTLLILVPFLFASVFVSCNRTTGKEENNTDEATESTSTSR
                     TKDPNIITVDDPFKNQPNVFCTVIRNLDISYYAPEKTVDDVAAYPKKCCLLDVCLDQP
                     GSSGMVVNLGDDTEIVTADFELIKVFDSADEARAYAEKHQIQDAIFE"
     gene            complement(16381..16878)
                     /locus_tag="PLM90_05730"
     CDS             complement(16381..16878)
                     /locus_tag="PLM90_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HMZ88903.1"
                     /translation="MDPRESARSRFKRQVTGLWTFRLFLLSKLPIALITGIRIRALDD
                     NSCTVSVPFKWLSQNPFRSVYFASQSMAAEMSTGVLSMMAIQGCEPPVSMLVTKVHAV
                     FTKKAAARVFFTCNDGEAIRKAVDQTMASGEGVTIVCSSIGKLKDGTIVSSFEVEWSF
                     KARSK"
     gene            16962..17240
                     /locus_tag="PLM90_05735"
     CDS             16962..17240
                     /locus_tag="PLM90_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HMZ88904.1"
                     /translation="MSAVFTQEDVLTEQLIDDLIGARLIVHNDDVNTFEWVIQSLVEI
                     CRHSQEQAEQCAYIIHFKGKYAVQQGAKRTLSPMREQLVDRGINATIE"
     gene            17246..17929
                     /gene="aat"
                     /locus_tag="PLM90_05740"
     CDS             17246..17929
                     /gene="aat"
                     /locus_tag="PLM90_05740"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HMZ88905.1"
                     /translation="MPIWQLDHRNIFPDPFDADPDGLLAIGGDLSSDRLLLAYRSGIF
                     PWFTLGHEPYWFAPDPRCILDFQNLHISGSMKQLLKKKAFDVTLDKDFESVIKSCAQI
                     ERKNDESTWIDNYFIDAYCKLHHQGHAHSVEVWQNEKLVGGLYGIIQGGVFFGESMFS
                     RVSNASKYGFISLVAHLQKHQFDFIDCQIYNDHLASLGAHNISREEYMLRLHNGLYKN
                     ATPVVPETL"
     gene            complement(17931..18575)
                     /gene="pdxH"
                     /locus_tag="PLM90_05745"
     CDS             complement(17931..18575)
                     /gene="pdxH"
                     /locus_tag="PLM90_05745"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HMZ88906.1"
                     /translation="MEQNEGKLHDMRTDYTTGVFDEQNAYPQPMQQFGLWFEEAIKAG
                     ISEANALVLSTVSNEGKPSSRVVLLKEYVSAGFIFFTNYNSRKGKELAANPYACMNFW
                     WGAMERQVRINGRMEKIPATESDAYFYSRPVGSQAGAMISPQSEVIEGREWLEQQFIA
                     QQLKGELKRPEHWGGYILIPEEFEFWQGRSNRLHDRLQYLKHSDGSWKIHRLAP"
     gene            18650..19243
                     /locus_tag="PLM90_05750"
     CDS             18650..19243
                     /locus_tag="PLM90_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ88907.1"
                     /translation="MRSWLMPFTRISAILLVLVCSLKHGEAQQYAQLSGIITDNSTGY
                     AVPYASVTVPDDIRGVSASEIGFFTIVVKPLDTLQFSALGYKPKLYVVPDTMHDDLVS
                     IAVSLQRDTIELDSFVVYPWPSREDFREAFLAYQETQPYVMQPIPGIRGKELIDTVPK
                     PPSPIMNPISFFYDEVVKPIQWRKPKRHMVDELPEWK"
BASE COUNT         5601 a         4031 c         3783 g         5967 t
ORIGIN      
        1 tacatctccc gtatttgaaa taacagtatc tgtgatcaat ataccggttc ccgccgaata
       61 cgttgtgttt tcatccgtag atgatactgt gaaatttgga tacgttccgg ttatggttgt
      121 tgcaccttca ccaattaaaa atactggttg atcgggtgag gtatttgtaa taacagctcc
      181 cgtaacatca attcccaatc cagcagcata ggttgtattc tcgtcggtgg aggaaattat
      241 gaaatcagga taagttcctg agattgtagt tgcgccatca ccggtcaatg ttaccggtag
      301 atcaggcaaa gtatttgtaa taactgttcc ggtaacgtca attcctgttc ctgaagaata
      361 aatggtattg ttatcagttg aattaattgt aaaatttgga taagtgcctg aaactgttgt
      421 tgctcctgtg cctgttaacg acacaatttg atccggtaaa ttattactta tcgttgtccc
      481 ggcaatgctt attcccgttc cggcactata agcagttcct gcatctgatg cccatcctaa
      541 atttcctgcg ccatcagttt ttaaatattg tcctgctgtg ccatcagttg ctggtaacac
      601 ccatatttta ttattaacga tattatccgg cgatttaaat cctacatagt tcacaccatt
      661 tgctgctaat tcaagaaagc gcaaaccgcc tgtatttcct gcggctgttc cgtatggatt
      721 tatttttatc gctgctgcat tggttgaagt aatatccagt gccgcattgg gagttgctgt
      781 tgatccaatg gctaaaaacc ctttgatata agcattgcca tttcctccct gccaaaaaac
      841 agctgtgcct gttccatccc gaaattcgta tctgctgttg gtagcactga aaaccaatcc
      901 gaagtttgga tttccctggt acctgaaagc aaacctgtcg ccgccaacta agtagtctgc
      961 attccctgta aaagtaaggt caccgtctgc taagaatctt gaataatctg tggttccacc
     1021 aaattgtgca atgccgccat tgacttgcaa taactgtgta ggtgttgtgg tgccaatgcc
     1081 aacatttcct gtagcaggga aagtgtttgt ttgtgtgaaa agatctcctg ccaataatag
     1141 cagacaaaga atgaggagta attttttcat agtttattgc tttatctcaa atgaaggcta
     1201 aagataaact aataacatgg ctatcgtttt attagatgaa aatttggata gtaaattcct
     1261 cgttgaaaat gttaagaaac gcctggctgc taccctgtaa tcttctgatt tcggctattc
     1321 tgccactgta attgtaaaaa atcactgaaa agtggtattt ctaaattcga taatgatagg
     1381 atgctattca agcccgaaaa attaatctaa ttgttgttaa gagggtatag cttttttata
     1441 tatgaaaaaa attaaaaggt cgaaccaaat atatatgcat tattaaatgg aaaacatata
     1501 ttagaaataa ttaaatgcga aaggtattta tggatggttt gcaattttaa ctttaataaa
     1561 tgtgttcttt agccggaaac tctcatgagg tttcagtatc ttgtttacaa ttgctgactt
     1621 tggggaagat ggactgtttt ataaatttaa tgtacgaaga ggggcatcaa tttatgttcg
     1681 cctgattagg tttcagtata atttccaaat attttaatca gtttacctca taactcattt
     1741 attatgcaaa caacaacaca cacaaccagt agatcaaatc aaggattggt gatccttgga
     1801 ttagtaatgt ctgtctcact tttcttctcc tgtgctgcaa aatctaagga agagcccgta
     1861 ctcgatggca gagttttcac cgtgctgacc attcctgatg gcaatagcgc agaggcagcg
     1921 atggagcgaa tttcttttac aaacggaaaa tttgacaacg ataattgcca tttatggggc
     1981 tttggcgatg gcgcttatgc tgcaaaagaa gccggtgaca gcattacatt tactgctact
     2041 accacaagcg ctaaagaagg cacaatgaca tggaccggag tcataaaagg agcaacatta
     2101 atgggcacta tggtttggtc gaaagaaggg cagtctgatt taaactacca tttcacaagt
     2161 gcaaatattg aggaagttga tctcaatgga ataagttttg ctgtgcaatt tagcgacgga
     2221 gatacggccc aagctgagac tatcacattt aataatggca tgtttgaaag tccgggttgt
     2281 tatacttatg gatttagtgc ttcaccctac caggcatata tgttggatgg caagcaacat
     2341 tttcaatgtt tatatactag tgcaaaagaa gggaaaatgc tattttatgg tgtaatagat
     2401 ggcacttcga tttcaggaac acaattttgg acgaaggaag gtcaggatga cgtttactac
     2461 aaaatgtcgg ggcagaagct ttaattatgg tagaaaaaaa gtcaggcagg aatatggttt
     2521 aatacctgcc tgatattttt ttattgaata atcaattgtg atgtacttac attgtcaccc
     2581 tgtcggactt cgataaaata caagcccttt gcctgttcac tgagatcaaa ggtgtgattg
     2641 acattgcctt gatttgacga aaaggtatat tgagtaatga gccttccggt aagatcgaaa
     2701 atgtttacca aagcatcatt ttcagaggtt ccttcataca ataaagagaa ttctccgaaa
     2761 gttggatttg gataaaccgt caacctggaa tcacttgatt ttattggttc gggttcttcc
     2821 ttaacgcctc caagtgtgca atcgtctaat cgggccttga actcacaatt cattgcggct
     2881 tgaaatccta cgtttaattt tacaaaggtt tctgcttcat acaggatttc attcgcactg
     2941 gtgcctgaaa ttgtagacgt gcttgttatg cttccgccac tgataaatcc tttatgacca
     3001 ctttgagctg cggaataaat gagatcaccg ctgcaattta cagtaaagta atgatcatag
     3061 gttttgccat caaatgtaac acgaactcta taagtgcctg tggtatagct taagaggctt
     3121 tgatcgaagt aataagtggc aatttcatca aataccaaag gggtataaga catgctgttc
     3181 ttaagcactc cggtgcttgt gaaaatgtcg acattgattg gcattccggc aaagatattg
     3241 cgtatcgaaa cgccaatttt tgcaatttga aatgaagaga agtgatttac caatgtttcg
     3301 gtctcattac ttcccgcttc ataatttgtg caactcgttt ctgttggtgc tccaaaatgt
     3361 gtagctaccc taatgagttg tgtattatag taaggaattt gattattcca gagagattga
     3421 tagtaagtga agttgcaatt gggttctcca acgtcttcat ttcctttcca tggttcaaca
     3481 agcgagccat accacattac ttgaaagtgt aagtgaggtc ctgttgaatt tcccgagctg
     3541 ccaattttac ccagatagtc gcctgatttt acagagtccc ctacatttaa atctgttagt
     3601 gtaccatgct ttaagtgacc atagagtgtc gtaagtcctc ctgcgtgatc aatgcgtaca
     3661 taattgccaa gatatccggt attgtcaagc gtatcattta taataaaagt atgcggctta
     3721 ctgcaatttc gatcaaaggt atttccatcg aatatcgcgc cgataacgcc atcagcagct
     3781 gcaatgacat ccacactttc atcatccatc atttgccatc cataagggta agggcaaata
     3841 tcggcaccgt tatggccttc ataatttctg gcataatcat ttgtgaagca ataccagtct
     3901 tctttaacag tggtatcagg atcaagatcg agataatttg aaatgtagaa atatgaatat
     3961 acaccggaca ggtctttata ctcatctgac atttttaaag gccaatcgag ttttggtgtt
     4021 aaatccattt tatttccggt tgttgtactg ttataataca ttggattgtt ggcatgtgct
     4081 aatttgccat ttggagcaag caatgaagcc ggaacttcct cgcaacctat tattttttcc
     4141 ggcctgctta tttcaacagt ctgcgcgggc agccattgcg tgcaaagcat catgcagatt
     4201 gcaataatgc ttttggtgat ttgatttttc ataatgatca gttgttgatt gaaataatta
     4261 attactcaaa gcatcaactt tagcttgcaa gtcggcattc tgcttctgaa gttcgatcat
     4321 atacaatgtc aactcttcaa tcttttccat cattagtttc tgcatttgac caacatttaa
     4381 tccttcggat tctacttcag cagctgatgg aatgccgggg aggcgattgt tttcatttat
     4441 gaaattttca acttcgctca atgaagataa tttataatca tctgcgaaaa cgtaatcagg
     4501 ccatggtgaa agttgaacca gtaattcttc gcatactact ttcccgtcta cacttaattt
     4561 atatccggat ttaggagcaa ctgttgtact gcccatcacg atgttaccat tttgatcaac
     4621 atacatactg tttataccat ttgcacgtag gataactttc cctgttacgc ctgaattagt
     4681 tgccagcatt aaatcagaac catttgcacg aaggtatcct acattcgacc cggcattttt
     4741 catttgaata tatggattcg ttcccataat tgtcacagtt tcatctgtgc catttactgc
     4801 gagcatagaa taaaggaaag ccccgctgac accaatattt agacgatctt gcacatatcc
     4861 tgacccataa acattgaaat tagcgctaaa tgcatccgga tcgccatgga cgccaacacg
     4921 gccaacttca tcaacaatta agtcagtatt aaaatctgtt ccgatgatac gtaatgcatt
     4981 atcagtatca ttgcctttaa tgcccacgag cgctgaaggt gatgaccagt taaaacctga
     5041 tttaccgcca tcggtaggaa ctatcacacc atagttattt gttgcaccag cacttcttgc
     5101 atataaaccg atgtttgttg ttccggcgcc aacacttttt gcaacaacac cataattgtc
     5161 tgatcctgtt gaactgttat tcgcatatac ggcagctgaa tttgttgttg tagaagcatt
     5221 gccccataca ccgacatgtg aaacaggtgt atcgccaaat actacaccaa cagtgttata
     5281 tcccaaataa ccggtagaat gcaccgttgc tgtgccacct aaacttaccg tttgatctac
     5341 acctttaata gcaatatctt catcggcaat aactccttca ttattgtaaa atgatgctgt
     5401 tgtgtttgat gaaataccat tgttccagcc aaaagaaaga ttgtcgccat catctacaat
     5461 tccgccggtt gtcaacgcgg ttccgtccca acgaggaatg gcatttgttg aaattgttcc
     5521 tacctgtgga tcagtttctg cgcctgctgc aggggtagtg gcaatccatg ctattccatt
     5581 ccattgcatc acctggccac ttgttgcacc catagaactt aagtcagccg cagcaattgt
     5641 accatttaag atatctgtag aagtaaccga tccatcagca atttcggcac ttccaactgc
     5701 tcctgttgaa atttgaaggt tgttataaac accggttaca tctcctccat gattagtcag
     5761 aattgttaaa tcatcagcag catttacatc gcctgtattt gagattacag agcctgcgat
     5821 tgaaatgcct gttcctgctg tataggtagc accaccacca ccggcagtag tttgtgtagt
     5881 gccatcaaga aacttaatat tatttacata taattcattc cagcgcaaag tgctacttcc
     5941 taaatctact gtattgtctg cattaggcaa taatggttgc gaaatagatg tagtggtgag
     6001 attgctcaat gttgtatttg caccttttga agcaatggca gtccatgatg ctcccgtata
     6061 atagtaaaat ccaggactgg agttagtttg aaaaatgagt agtcctgttg caggtgatgc
     6121 aatagcatct ctttgtgatt ttgtcatacg tggcgccaat aaccctttgc tggttgaaga
     6181 catttccagc aatgctgatg catttggggt tatggtgcca atgccaacac ttcctgtcgc
     6241 gggaaatgta ttggtttgtg cgtttacagt gagtagtcct gctataaaac acacggcgaa
     6301 tagcgtaatt tgctttttca tagaatagtt tttgtgatag gttaaattgt atcagcaaag
     6361 ctatttccat gaatgcacct gtaaaatacc gagaatgggg gagtgggtca aataggaaaa
     6421 cgcacacgaa tagtagtgcc tgtatttggc gtggagttaa tctccaaagt gccagctaat
     6481 tcctgtgctc tcatttgcat gctatcaagc ccattaccgc gtctgatcgt ttgcaaatcg
     6541 aatcctttac cattatcggt aacgattaat tgcacatgat gatcgacata tgagatatgt
     6601 gcttggatat ttgttgcagc agcatatttc accgcattat ttacggcttc cttaaatatc
     6661 agcatcaggt ctttgcgctg aagcatattg agcttgatat tcggatctat gttcttttgc
     6721 aggttgaatg aaatttcgcc aggagaacac atgtcaaaca tgaaattttc catccgcgat
     6781 aaaatgccgg ataactggtc atgcttagga ttgatcatcc atatgatatc atgcattttt
     6841 tcagcggttt ctgatgcatt gacctggatg cggccaagaa gatcatcaag ttgatgttta
     6901 tcatcttttt gtaatgccgc aatctcactg agcattttta tggaactcaa cgaacttcca
     6961 acttcgtcgt gcaggtctat cgctatatca ttgcgcatct gtagttgctt tacctgctgc
     7021 ttgatccggt atctgttcca tattaataaa ccaaaaccga gcaacatgat aatcaggcca
     7081 atcgctatat tgcggaacat cacctgacgc tcattttcgc gcaattgcaa ggcgttgctg
     7141 gtttctaaaa gctcaatgcg ctgttctttt tgggttgttt caaattcttc ttttaatctc
     7201 agtacttctg attgttgtga ttctgaattc aagctgtctt tcaaagctat gtaaagctta
     7261 taattttcta aagcttccgc cggccgcccc atggaatcgt aaatacgaaa atttaaatca
     7321 taagacgcca atttgaaatt tacggcatca tacttggtaa ataatacaag tgccgaatcg
     7381 ttgtagctca atgccttatt atattgcttg gcaattcgat atacgttggc aattttatag
     7441 tattcgtaac ctacatcggc acctgattgc aatgcttcca tgatctgctt tgcacgctga
     7501 aattgtttga ttgccttttc cctttcatca ataactaaat aatcatcacc cagattctcg
     7561 atgagtattg ctaaattgta agaaacaggt tccttttcca ataaatcgat agcctttgag
     7621 tgatagtaaa ttgcgctgtc tatttcatat tttaccagaa ataaactacc aattcctgaa
     7681 taggtcattg caatcagcga atttttgctg ctaatgccaa agtttgtccc tgattcatat
     7741 gttttaagag atttttgaag ataagaatag gcaagatcat agtcttgaag gtcgcgataa
     7801 atatttccaa tcgataaata aatagctccc aatgctacag ggtctgaact tttttctatt
     7861 gcttttgctg atttaatttc atattccaga gcagtattta gattgcctaa aaacttataa
     7921 gacaatccga tattatgata tgttcttgat aatgctgaac tatcgctttt taaatatggt
     7981 aattgttttg taagatgatc aatagaaatc tgatacatcc ctttgctctg ataagccaac
     8041 ccaatggcgt tataagccgg agctatctgt tcttgaaggt tatgcttttc agataagtcc
     8101 agtgccatgt ttccgtaaaa tatggcacta tccggttttg aatatgcaaa ctgatatgcc
     8161 agatcattat aggtatctgc aatggtttct tcatccttgg tgtttttaat caccttccgt
     8221 agtgaatcaa tatcctgctg tgcagatata ttatttatcc aacaaatcga taacaaaaaa
     8281 atgtaaatgt gcgctgtcca tcttgtcata cgatactttt gattcaagcc tgttagatga
     8341 tcttatcctt tatcgccttc accaccgctt ctgtatttga atttacatgc agtttggtgt
     8401 aaatattctt catatgggtt ttaacagttt cagtagagat tgacatttct tcagcaatca
     8461 ttttgtagct cttgcctttt accaagttct gaagtatctc tttttctttg tctgtcagat
     8521 tgtagttcac atccacctgt gaagctagat tggtcttgaa taattcaaac acctttcgag
     8581 caatcgatgg actcataggt gaacctccgg catgtacatt tttgatggct tcagtgtaac
     8641 ctaatggcgt ggtggttttt aatatatacc ccgatgcacc gctgcatatc gcttcaaaga
     8701 tatagtcatc gtcatcgaac atggtttgca tcagtatctg aacaccggga aagtgctgtt
     8761 tgataagttt tacggcctct atgccattca tgccgggcat ttcaatatcc atcaaaacca
     8821 catccggaac atctgattct atataaataa gcatgtctct gcaatcagga taggttccta
     8881 tacaggtaat acccggagtt gcggaaataa gcatttgtaa gctctccctg atatgcttgt
     8941 tgtcgtcaaa taaaactaca cggatgtcca tcggctgcaa aagtatagtc tgattaatga
     9001 cctaaacata ccgaatttgg gtgatataag gatattcgtt tcctttgaac agttgtattt
     9061 tatccggaat tagccgattt acactatcgc aaagccggtt cccaaaaaca aaacggcgac
     9121 caaagtatta aacaatgatc gccgtttatg agaagaaatg tgattaattg ccaccaatag
     9181 taccattggc atcgcaaaca ggtggacata aattggtgcc tgttcttgca gaagaataca
     9241 tagtatctgt ttggtcatta ccctgaccat ccattgccac tgctatcgtc acgtcgcctc
     9301 cgttatcgtc ttttccaaaa tacaagcgga atttatttgc attggggtgg gctgccgcaa
     9361 ctgtatttaa ggattccaat tggccagcct caataacgac tcccttgaca acagctttta
     9421 ctttttcagc ctgatctgca taattagcta caaatcttgc agcatcctca gttgagatcg
     9481 gttgactggt aactacctgt tgtgccggta gtggttcagc agcttgagaa gtaagcacga
     9541 tagttgtagg gatgatggtt gtaattgcga ggataatggt agctttctta taccatgaga
     9601 gttgttggtt agtttgcata atgtgagttt ttggttgtat gtttggttaa agatacaaac
     9661 tataccggca tacacatttg acctaatcac aagactcctt acttcaaaca tgaccatgca
     9721 caccaagctg tccgggtttt tcacctggat cgttttcact attgtaccct ttattggatt
     9781 cgccgaaaca gagcccgggc ctaataacaa tgccgtaacc tacaatctgc ctgttattga
     9841 taaatctcac atagaaagcg acctaaatca cgcaacagaa acgttgcaac gtatatcgct
     9901 gcttggtgaa cttgcggcct attatgttac atgggaagct gatgcacata aggccgatag
     9961 cctactcagt gtgggaattg acatggctga actgtcgtat aacaatgaat tcttacttga
    10021 agcgtattcg aattatctga tttatattga tgattatacc ttcacagata aagtgagtcg
    10081 catagtccct ttattggaaa gcatgtcagg tcagcttacc aataaaaaag aggtatggaa
    10141 atgtaagtat gctcttgcct atgggtgtaa actaattttt caaacagatc gtgcaaagga
    10201 ttatgcacac caggcattga cagaagcaat acgcttaaaa gataacaacc ttaccgccga
    10261 aagccatctt ttgctgggct cattgcaaca tgatatgaat aacaacgttg aggcaattcg
    10321 taattatctc gatgccttga ccattgcgga aaatggtagc gatctggagt tgcgtctgaa
    10381 atgctatgat gaactatcta cattttataa tttgataaaa gcttatgaca agtcgattaa
    10441 ctataaactc aaagaattaa atattgctga acatgcaaaa aagccggatt caatgcgtat
    10501 aatgaccatt aaatttgata tggaagttat tgcatttaat aatagaacct taaacgaaaa
    10561 tcagttatat aaaatcattg attacagcaa tcgaaatggc gtcagtcgcc tgaaacgatt
    10621 tgccctggtt gtgttccgaa accatcttat taaacaaagt aattttgcgg aattatatca
    10681 tttgtacaat gaggaatatc cggaggaact ggaatatatc aggactaacg acacaacaac
    10741 ttatttcagg ctcgaggcaa tgttcaatga atatgaaggg gatgttgtaa ctgcaaagga
    10801 atattttgat aaagcagcac aagggattaa caggtcaaag gataaattgc gtcagtcaag
    10861 cttttatttg agatatggcg attttctcca acgcaataat tttctggaag aagccagaga
    10921 ttgtttccga aggtcatata ctatcgcagc atctataaat tattatgaat ttatggttga
    10981 agcaacaggt aagctcgaga aaatttattc acttcaaaat aatttcgagg aggcttacgc
    11041 atatagtttg ctgaataaaa gcacctcaga aagccttgat agcttaatgc aaaaagaaca
    11101 gttgcaaatt ctggaactgg agaatgaaga agtgctgcgg gatcaacaac tgcaacaaga
    11161 acgcgatgag accagaagac gtaacaatat tcaatacacc gctattacta tccttatagc
    11221 tacctttttt ctgatgcttt tcatacttgg agggatcagg gtttctcctg caatcatcag
    11281 gtttctcgga tttgtttcct ttatcttctt ctttgaattt ttaatcttgc tgttcgacac
    11341 atggatacat catttaacgc acggtgagcc atggaaaatt ttggcgataa aaattttact
    11401 gatgtgcgga ctggttcccc tgcaccatat gattgaaaaa agggtgattt cctatattat
    11461 cagccataaa atgaatctca taccaaaacg ctcgccaaaa cctgtattgg ttgaagccga
    11521 tgatgtgagg gaagaggaaa aagcttgagg ccgacgcact tattaaatac atagaatttg
    11581 gcttgtgagg tgttcaaatt atttttcaca atggaaatat cttgtaattt tgatatcatt
    11641 tattttaaca aggtatgata agcatttcta aactaatttc ttgtggtatt ttttcggtgg
    11701 tactcatgtg ttgtagtatt gaaatgtcat ttgcacaaac ttttgctgtt gatcttgaaa
    11761 atccgatctc tacttcattc tcttttttat caagtacacc aaatgcagga gttaatgcaa
    11821 tgggtgaagc cggcactgca gtgataaata acggagcttt gtacacaaat gctgcagcaa
    11881 tcagtgcatt gctcgattcc tttggtctcg ctctcgatta taaacgcggc gccggtaact
    11941 tgttgtgttt gtcggtatac aatcaataca cttcaagcgc tcttgcattt ggattgcgat
    12001 ataattccct gggaggttca acattgataa caccaacagg aaatatcaac cagggtgcat
    12061 cgaattatga attggcattt gatgtcgccg gatcgcattc attttcgcca cattttatgg
    12121 ctgctattac agggaaagta ttttattcaa ccatttctaa agacatgatt attaatggtg
    12181 ttgtgatcaa accttcatca ggaatagctg tagatatttc ctgcttatat acaacaacag
    12241 ttaacggaac tgataaaatc aatattggtg ccaccattag taatatcggt agcccacaag
    12301 aatatggata tccgggctat gattatttta tcccgactac acttagtatt ggctttgcat
    12361 atgatgtact ttttgatgac aataatcaac ttgtatttgc attagatgta aataaactga
    12421 ttgttcctac aatatacaat agatcagaat ctgccgcata tggcatgtta cactccttta
    12481 atgatgcccc gggtggagtg caagaggaaa tgcacgaaat tactttggga gcgggaatag
    12541 attatagtta tcaggaaaaa gtacaccttc gggcaggata cttttatgaa catccctcta
    12601 aaggcggacg cacctatttt tccctgggcg gtggcatagc aataggcaac ggaaatctgg
    12661 atgtagctta tcgtttttat gacaatacac aggcaattgt tgtagcaaat gcactttcgc
    12721 tcacactgtc ggtaaacctc taagctttat aaaatttgcg tttgaatttc caaaggattc
    12781 ccgccttcag catgaaatcc caggatgatg ctccctcaat ggcatcatta tgatattgcg
    12841 gagtcgtatt atatctacct gcaaatccgt agtgcacaaa aaattgcttc ccgaatacat
    12901 agccgatatt atatccaagt gaccagccca tagaataggt gtatgtagaa tcacctatca
    12961 atacaccatt atacatgttt acatcagcat aatgcagcca attacttgtt acatatgtta
    13021 cactctccgc atttggtgca aacataaacc ctgaaggtgt tctggtaacc catgtcaatc
    13081 cggttgccac atatgcagca tgtgcaccat caacattgcg ataatcaatt ccgatattat
    13141 aatttattgg ccatttaata aaattcaccg gcttgctata aattaattct gcgccaatgg
    13201 cctttgaaat atccggatca tataatccat cgctaataaa ttgtgatgca tttgtagcgc
    13261 gaagtgaaat gaattgggcg ctgcagtcaa atgttactac aatgctgcac aatagaagga
    13321 ggtttctaat cttcatgcaa tacaaaacgt taaaagatta ccggttattt ttaattcgcc
    13381 acctgacctg tcgtaatgca atgagcaaat aacccagtat cacatattcc acaaatctta
    13441 ttcccaatac ggcaaaaata ttgatcgtct tggctgcttt cccggtgcta tcataacaag
    13501 tatagtcctg tgtaatattc gtttgtcccg ggattggatt ggtaacatct atatctcttg
    13561 taaggctggt tccatcatga caaaaacaca tttctatcgg tttattgtat aagaaattgc
    13621 ctgtttggtc tactgtggct cccatgagtg caaaaaagct cagtccgata gctctggcaa
    13681 aacttccggc tctgtcaggt cgccgaaggg gtataacgcc aaatccaacg attccacaaa
    13741 tcaaaatcac aatactggca actccctcca ttgttgcaat tgctatgaaa gaaccgatga
    13801 gcgttaaaat caaggctgct atacccccaa ctatcagaat gatccaaacc caggcaggta
    13861 gccgtttcca tatgctttta ggcttgtttt ccggaactcc gttcagagcg ttaccacagt
    13921 atctgcaata gctgaagtcc accgggttct cgttatgaca atgtgggcag gttttcatgc
    13981 aagcaaaaat aggatatctc cattaatgca ttcttcctta cctttgcagg cgatggaacc
    14041 cctgtcaaat caacaagatc agcaacaaca acacaacccg aacctctttt gggaaggctt
    14101 tttttacttg agcttcttcg tgtttttcga tattgttgta atcccatatg cccctaaatc
    14161 tataaccttc gatcaatatt acccccttgc tatggtaatt gttggcggcg cagggctttt
    14221 tgttggaatg ctggcgttta gaatcatgaa aaactggccc aattttatta aagctggcct
    14281 ctttgtggtg atgtatggta tcatcctgtg gtatcttatc gaaaacatca acaaattcac
    14341 ggttccgtgg atgtaatgcc actttcttta cattttagat aaatttcgac ccttatcatc
    14401 cttgcacttt aaaaaaagtg tagcccaaca ggcatatctg tgcatacatt ttataccttt
    14461 gtatggcaaa tttttacact accaaaccaa acgtgcacga agattatgtc tgagacagcg
    14521 catatcaaac ttggagaaca agagatagac ttgccggtgt ttaccggttc cgaaggagaa
    14581 caatcgatcg atatcaacaa attgcgcgac cttaccggtt acgttacgct cgatagcgga
    14641 ttcaaaaaca caggtgctac taaaagcagc atcacttttt tagatggtga attgggtatt
    14701 ctgcgttatc gtggttatcc gatcgaacaa ctcgccgaga agtcgaactt catggaagta
    14761 acatatctgt tgctttatgg cgaattgcct accaatgagc aatatatcga attcactgat
    14821 cgtatcacgc gccatacact tgtacatgaa gacatcaaac aatttttcga tggcttcccg
    14881 tctaatgccc atccaatggg tcagttgtgt tcgttgatgt gtacgctcag ctctttttat
    14941 ccggatgccc tgaatcctca tatgagcgcc aacgaggtgg atatgactat tacccgtatc
    15001 ctcgcaaaaa tgccaacact cgttagctgg attcataaaa aatctgttgg tcatccaatt
    15061 atgtatccgc aaaataaatt cgactatgtt accaatttcc tgtatatggt gtttggtatg
    15121 cgtaccgaag aatatatgcc ggatcctgta gtagtagatg cacttgataa attgcttatc
    15181 ctccacgccg atcacgaaca aaactgctct acatcaactg tgcgcatggt tggttcaagc
    15241 caggctaatg cttatgccag cgtagcagca ggtgttgccg cactttgggg gccacttcac
    15301 ggtggtgcca accaacaagt aattgaaatg ttggaagtga tcaaacgcga tggcggaaac
    15361 gttgcgaaat atgtagaaaa agcaaaagac aaaaatgata atttccgttt gatgggattt
    15421 ggtcaccgtg tatataaaaa tttcgaccca cgtgctaaaa tcatcaaaga agcatgcgat
    15481 aacgtgttga ataaacttgg tatccatgat cctgttctcg atatcgctaa acaacttgaa
    15541 gaagtagctt tgcgcgataa ctattttgtg gaaagaaaat tatatccgaa tgtcgatttc
    15601 tattccggta tcatctatcg tgcaatagga ttcccaactg aaatgttcac cgtgttgttt
    15661 gcactcggtc gcttaccggg atggttgtca caatggaaag aaatgcgtga atccggtgag
    15721 cctatcggtc gcccgcgtca aatctataca ggaccaacac aacgcgatta tgtgaatatc
    15781 gctattcgtt gagataaaca gccttaacca aataataaag gagacttgct tcatcaggtc
    15841 tccttttttt atatgctgaa atacaatttt aattactcaa aaatcgcatc ctgaatttga
    15901 tgcttttcgg cgtaagcacg cgcttcatca gcagaatcga aaacttttat cagttcaaag
    15961 tcggcagtaa caatttcagt gtcatctccc aaattaacaa ccattcctga agagcccggc
    16021 tgatcgagac aaacatccag caggcaacat tttttaggat aggcagcaac atcatcaacc
    16081 gttttttcag gtgcataata actgatgtcg agattcctga tcactgtgca aaacacattt
    16141 ggctgatttt taaatggatc atcaactgtt ataatatttg gatccttggt gcgtgatgtt
    16201 gatgtgcttt ctgtagcctc atcagtgttg ttttcttctt tgcctgttgt gcgattacag
    16261 gatacgaaaa ccgaggcaaa cagaaatgga acaaggatta aaagagtctt gggttgatgc
    16321 atattgcgat gattttcttg aagtgtaatt attaataaac gtaaatagta accataaata
    16381 ttatttgctt cttgctttaa acgaccattc aacttcaaaa ctgctgacaa tagtcccgtc
    16441 tttcagtttt ccaatactac tgcaaacaat agttacgccc tctccgctcg ccattgtttg
    16501 atctacagcc ttacgaatgg cttccccatc attacacgta aaaaataccc gtgcagcagc
    16561 ctttttcgtg aagacagcat gcactttcgt caccaacatc gaaacaggag gctcacatcc
    16621 ctgaatggcc atcatactta acacaccggt cgacatctct gctgccattg actgcgaagc
    16681 gaagtatacc gatctgaaag ggttctgcga cagccatttg aacggcaccg aaacagtgca
    16741 gctattgtca tccagggcgc ggattctgat gccggtgatc agggctatag gcagtttcga
    16801 caacagaaaa agccgaaagg tccataagcc tgtaacctgc cgtttgaagc gggatctggc
    16861 ggattcacgt gggtccataa ggctcgaaat tagccaactt tttgaaccag gagccatgaa
    16921 taactgttcg tttgttaatt ttgtatccta aagttcaagg catgagcgca gtatttacgc
    16981 aagaagatgt attaaccgaa cagttgatcg acgacctgat cggcgcacgt cttattgtac
    17041 ataacgacga tgtgaatacc ttcgaatggg ttattcaaag cctggttgaa atctgccgcc
    17101 actcccagga acaagccgaa caatgcgcat atatcatcca tttcaaaggg aaatatgccg
    17161 tgcaacaagg cgctaaaaga acgttgtcac ctatgcgcga acaactcgtt gatcgcggaa
    17221 ttaacgccac catcgaataa gcgaaatgcc aatctggcaa ctcgatcatc gcaatatttt
    17281 tcccgacccg tttgatgccg acccggatgg tctgctcgct atcggcggcg atttatcttc
    17341 tgatagattg ttgttggcat accggtccgg aatttttcca tggttcacct tgggacatga
    17401 accgtattgg tttgcgccgg atccgagatg catattggat ttccaaaatc tgcatatctc
    17461 cggaagcatg aaacaacttt taaaaaagaa agcgtttgat gtcactttag ataaagattt
    17521 tgaatccgtt attaagtcct gcgcgcagat cgaacggaaa aatgatgagt ctacttggat
    17581 cgataattat tttatcgatg catattgtaa actccatcat caaggacatg cacactctgt
    17641 agaagtttgg cagaacgaaa aactcgttgg aggtttatat ggcattattc aaggcggcgt
    17701 ttttttcggg gaaagcatgt tcagtcgcgt aagtaatgcg agtaaatatg gctttatttc
    17761 actcgtagcg catttgcaaa aacatcaatt cgattttatc gattgtcaga tttataacga
    17821 tcacctggca agcctgggag cacataacat ttccagagaa gaatatatgt tgagattgca
    17881 taatggttta tacaagaacg caacccctgt tgtaccggaa acactgtaat ttacggagcc
    17941 agtcgatgaa ttttccagct gccatcgctg tgtttgaggt attgtaacct gtcgtgtaat
    18001 cgattacttc ttccttgcca gaattcgaat tcttcaggta ttaaaatata acctccccaa
    18061 tgttcgggac gctttaattc accctttaat tgctgcgcga tgaattgctg ttccaaccat
    18121 tcacgacctt caattacctc actctgtggc gatatcatgg cacccgcctg actgccaaca
    18181 ggtcgtgaat aaaaatatgc atcactttct gtggcgggaa ttttttccat cctcccattt
    18241 atgcgaactt gacgttccat cgccccccac caaaaattca tacatgcata tggattagct
    18301 gctaattctt tcccttttct gctattataa tttgtgaaaa aaataaatcc tgcagacaca
    18361 tattccttta acaaaacaac gcgcgaactt ggtttaccct cattacttac cgtcgataac
    18421 acaagggcgt ttgcttcgct tattccggct tttattgctt cctcaaacca caatccaaat
    18481 tgctgcatcg gttgtggata ggcattttgc tcatcgaaaa cacctgttgt gtaatctgtg
    18541 cgcatatcat gcaattttcc ttcgttctgt tccattgata tttgaattaa ccttgtttaa
    18601 catgcaaatc ttacaaatta tttcataatc catcataact tgcgtcacca tgcgttcttg
    18661 gcttatgccc tttacccgaa tttcggctat tctattagtc ctcgtgtgct cactgaagca
    18721 tggtgaggct caacaatatg cgcaattgag tggtatcatt accgataatt ccaccggata
    18781 tgcagtgcca tacgcatctg taactgttcc tgatgatatc agaggggtgt ctgcctccga
    18841 aattggtttt tttacgattg ttgtcaaacc tttggatacc ctgcaattca gtgcattggg
    18901 gtataaacca aaactatatg tcgttccgga taccatgcac gatgatttgg tgtccatagc
    18961 ggtttcccta cagcgcgata ccatagaact cgattctttc gtcgtttacc cctggccttc
    19021 aagggaagac tttcgcgaag cgtttttagc atatcaggag acacaaccct atgtgatgca
    19081 gccaattccg ggtatcaggg gtaaagaatt gatcgatacc gtccccaaac ccccttcacc
    19141 catcatgaac ccaatttctt tcttctacga tgaagtggtg aaacccatcc aatggcgcaa
    19201 acccaaacga cacatggtcg atgaactccc cgagtggaaa taagcactca ccactcacga
    19261 ctcacgactc acgactcacg actcacgact cacccactca cccactcaca actcaccact
    19321 cacccactca caactcacca ctcacccact ctcccactcg ccactcacca aaacggtgac
    19381 at
//
LOCUS       DAOQOU010000044        18842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1468_length_18842_cov_5.289775, whole genome shotgun sequence.
ACCESSION   DAOQOU010000044 DAOQOU010000000
VERSION     DAOQOU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_18842_cov_5.289775"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLM90_05755"
     CDS             <1..332
                     /locus_tag="PLM90_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88908.1"
                     /translation="LAGYPKSELPGVWMEITKDAKDGYNIHFNTTNFQFAPDKVNTEH
                     VMGEGSINLFINGDKVARVYGEWYHIDIAAPGTYEVVATLNSNELSDYCIRGTPISAR
                     EKITVSR"
     gene            391..1119
                     /locus_tag="PLM90_05760"
     CDS             391..1119
                     /locus_tag="PLM90_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ88909.1"
                     /translation="MTEVDLFIPCFIDQMFPETGFNMVKVLEKAGCRVHYNPNQTCCG
                     QPAFNAGYWKEMEDVGKKFLADFANSDRYVVGPSGSCVGFVRNFYGELYANTPVHNYH
                     KNLKKNLFEFSEFLVDVLKVEDFGGKFPAKVTYHDACGALRECKIKEAPRKLMSHIEG
                     LELVEMQFPETCCGFGGTFAVKFEPISTGMADSKVNDAIASGAEYIVSTDYSCLMHIQ
                     AYIEKQNRNIKVLHIADLLAMSIA"
     gene            1164..1829
                     /locus_tag="PLM90_05765"
     CDS             1164..1829
                     /locus_tag="PLM90_05765"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HMZ88910.1"
                     /translation="MDEINQIASDIRRYQENGMRLFTSSSFQTHSTVLLHIISRIDPT
                     IPVFFLNTGFHFPETIQYKNEISQQFGLNTIDLRAATPKLMQVDDHGRFLFTSDPDYC
                     CYLNKIQPLEPMLDTYDVWINGVRADQNANRATFKKEAPTAHRAVRYHPMLGWSGKMI
                     YAYIREHQIPHHPLDAKGYTSIGCEPCTRKLSPEIMADERQARWYGMSKTECGLHTEL
                     IKK"
     gene            1826..2752
                     /locus_tag="PLM90_05770"
     CDS             1826..2752
                     /locus_tag="PLM90_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="HMZ88911.1"
                     /translation="MKILITGGAGYIGYALAQRLSENDKVTEIILYDNLSRHNEIIFF
                     GNAIGNGKLKLIKADILDARKLRNAMKGVDVVYHLAAKVLTPFANQDPHFFEQINHWG
                     TSELVEVIEKSEVKHFIYLSSASVYDRVSEPATEDTIPTPNTYYGISKLRGEDHIKRI
                     PASTKTHIIRGGNVYGYSPSMRFDAVINNFLFQANFEHKLSIHGTGAQTRAFIHIDNA
                     SDALAQIPFSELPSGVYNMVTKNLAVNDIAEVICTLYPQCDRIFINQHLQLGSIRVST
                     DSPFWKYIQVEQRSLMEELADFKAKFAFSGWD"
     gene            2743..4665
                     /locus_tag="PLM90_05775"
     CDS             2743..4665
                     /locus_tag="PLM90_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HMZ88912.1"
                     /translation="MGLISSMHDIHAILKKHWGYDQFRPAQEQIIRAALDGRDTLALL
                     PTGGGKSVCFQVPALAMDGICIVVSPLIALMKDQVHHLKQREISAAAIFSGMSFREVD
                     MTLDKCIAGDIKFLYVSPERLLTPIFRERFKQMKLNLLAIDEAHCISQWGYDFRPPYL
                     QIAALREFFPKVPVLALTASATPEVCEDIMLRLQFKEKNLIKGSFLRNNLSLIVRQQE
                     NKEDKLLEILRSVPGTAVIYVRNRRKTKEVAQFLMRHQINASWYHAGLDQETRSNRQD
                     QWINGKLRVMVCTNAFGMGIDKSDVRVVVHLDIPESLEAYYQEAGRAGRDGKRSYAGL
                     LFDIADVKVLDENYQKQYPEIAFIRQVYHQLGNYCKLALGAGENATFDFDMVAFCRQF
                     QLPAVETRNALRILEQHNVIYVSDSLFKPSTIFIPVDKETLYRFQIENKQFEPVIKLI
                     LRTAPGVFDDHTAIQETSMAWHLTITQPKLQEILQYLHDQEVLIYSPVKTKPQITFIH
                     ERIQASTLQLDYALLEQLKNAALNRLHAIKAYVNNHTDCRSVKLLQYFGEETPSCNVC
                     DICVEKNKLDLTEKEFRRIFDWLKEKLAGNALPPDALFQDKLPARKEKILETIRFLAD
                     NKQILHTKDNILIWQE"
     gene            4662..5747
                     /locus_tag="PLM90_05780"
     CDS             4662..5747
                     /locus_tag="PLM90_05780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88913.1"
                     /translation="MKHIVCTVTNDLTYDQRMDRICTSLTKAGYFVTLIGFVKTDSID
                     TLQKPYAQVRLRLMFKKGKLFYLEYNFRLFWYLLFRRFDIYNGIDLDTLLPVFVVAKL
                     KRKPIVHDAHEYFTELPEIVDRPVIQRAWKWLAKRILPHVKNNYTVGEAIATALQDSY
                     KNVYHVIRNVPVLNGNAPIEKGDYILYQGALNKGRGLEALMQAMQMVDAPLYIAGEGD
                     LSDKLRNMAATLPHANKIVFLGYIRPDELKTFTAKAKIGINFVEPLGLSYYYSLSNKF
                     FDYIHAGIPQVTMDFPEYKRLNDLFNIALLIDEITPEKIAAALNTLLNDAVVYNKLAA
                     NTQAAKRELNWQREEEKLVKLYRQFEK"
     gene            5737..6861
                     /locus_tag="PLM90_05785"
     CDS             5737..6861
                     /locus_tag="PLM90_05785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ88914.1"
                     /translation="MKSKHLHIISFNVPYPPDYGGVIDVFNKIKALHAEGIKIHLHCF
                     EYGRNEAPELNAFCEHVYYYPRLPMWRGFFSRKPFLIASRKSNALRNRLLADEHPILF
                     EGLHACYLLDDPAFQNRIKLVRMHNNEPVYYKTLANRENRMFKKTYFLTEASRLSRFE
                     SVLSHAQHILCISPYEADHYEIRFPQTTYISAFHGHEAIHSKPGKGSFVLYHGNLSVN
                     ENDEAVKFILKEIWKELNVPLVIAGSAPSEILVRLIQEYPQVKLVQDPDQNTLEELIR
                     NAHIHLLPAMQQTGIKLKLLNAIFNGRFCIANRNMIEHTGLEQYCLCFNHADEAKAAI
                     NAYMQQEFTAEDVIFRQPVVDTFSDSASAKKIISYLPEHP"
     gene            complement(6836..7531)
                     /locus_tag="PLM90_05790"
     CDS             complement(6836..7531)
                     /locus_tag="PLM90_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ88915.1"
                     /translation="METTSGNDIHFSGVSIFQGKTLVLSDVTFSIPHGEFVYLIGKTG
                     TGKSSLLKTIYGDLSLRSGSAEVAGFDLAKITWKTVPFLRRKLGIIFQDFQLLMDRSV
                     DDNLEFVLRATGTTDRSLIKLKSDEALKRVGLQSKGFKMPHELSGGEQQRVVIARAII
                     NDPVAIVADEPTGNLDPETSDDIMQLLRFLNKEYNTTILMATHNYNILEKFPARILKT
                     ANGKLITDVPVNS"
     gene            7616..8650
                     /gene="hemE"
                     /locus_tag="PLM90_05795"
     CDS             7616..8650
                     /gene="hemE"
                     /locus_tag="PLM90_05795"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HMZ88916.1"
                     /translation="MALSNDLLLRVANRQSVERPPVWLMRQAGRILPEYRALREKVQG
                     FKNLVENPELVCEVTLQPVRVLGVDAAILFSDILVVPEAMGLPYQMVESKGPWFENTI
                     DSPSDLSNLQIVDPEASLGFVLDGVKLIKGELNDSIPLIGFAGAPWTIFVYMVEGRGS
                     KTFSKAKAFLYRHPEASHKLLSMITEATIAYLKAQIKAGVNLIQLFDSWAGILSPEQY
                     AAFSMPYISRICNAIDEVPKTVFPKGAFFARQALSEINCNVIGLDWNMDIAETKQIVN
                     GRKALQGNLDPCVLYAEASVVKSETLKMLRAFGKDHIANLGHGVYPDTPLDNVKCFVD
                     TVKEFAYTGE"
     gene            8757..10055
                     /locus_tag="PLM90_05800"
     CDS             8757..10055
                     /locus_tag="PLM90_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88917.1"
                     /translation="MRTFYPKFLFIVTFLGLTVFAEGQGYVGIGTTTPAEKLDVNGAI
                     IVRGDATAATPVAGTVRWNSTDGMHDGRTSAGEWRRLENNFSLLSGTYYNVSCGATLT
                     TTVGAGTSTSATTYDTPFGTQYADNKRDYLYFASELTAAGYCAGTITALGFEIVSLGT
                     STINNFQIGMQSTALTAISGTTFAGGYTIVYGGPGTTISLTAGVNYFTLTTPFTWDGT
                     SNILIQVCFNNGGVGYSGTNSSVKCNTTGVTVKTRYYYATLPSDGCTMATGTSTAVSR
                     PNLYVTGKTTGPIGTSGNYLYSALPWVVGTPAIPAPYVHHGDGTVTAQSLYDDNTLLS
                     DHVFDYYFDGKVADQDLALHSDYKMASLEEMIAFMEEQRHLPTIQGRSAWEQNGKFSV
                     GQLSTELWETSETQSLYILELNKRIEALKAKSDSLEPTKK"
     gene            10061..11467
                     /locus_tag="PLM90_05805"
     CDS             10061..11467
                     /locus_tag="PLM90_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88918.1"
                     /translation="MTTHLRAFIFALLLLPAITVFGQGEVGFGTDTPAEKADINGAMI
                     IRGAAVAATPILGTIRWNSTDGMHDGRETPGVWTRLENDEFEYNGDYTAMVCGTTSTT
                     SAGTATGTSTTASETPFATAYSDKRSQYLYYGTEIALGGLCAGYITQIGFQVSVLGAP
                     AALNGLTVSMKMTTTTSLSGLTWDTGLTTYYNGNYTLGPGPNYISLTSGAYPSGFYWD
                     GYSNIELELCYDNVSSSFNTTVEISSGLAYTATRTHYASPGVGCALTASVTEAKHPVL
                     YLTGTGVGPTTGVGDYLQFNLPIMIGSPVLPAPYVHHGPGSITAEAIYDENVIISDYV
                     FDNYFDGEMSETDKVKHADYTMRSIDEMTEYMRIHRHLPTIRGRNDWEENGKFSTGEL
                     ASELWVTYETHALYLKELHERTLVAEQLLMTSDEILKDAYLAEIFRLNQDNTLTESVK
                     SERIALLNQKIEELNHAK"
     gene            11478..12896
                     /locus_tag="PLM90_05810"
     CDS             11478..12896
                     /locus_tag="PLM90_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88919.1"
                     /translation="MKGIYLAAFLSVASLSAFSQGYTGIGTNTPQERLDVNGAIIVRS
                     DAVSPTPVPGTIQYNTTDGYHEGYTSAGTWIKLENENTIGYGDYITVVETCTSPATVG
                     SNEYTTPSTGYFNPNYEENPFSTWWMDDRTQLLYKASQLTAAGICAGNINAIGFNVVT
                     PGAYAMTSLTIKMKSTNITTLTAYETALSTVYSAGSYTPVVGANDFTLTSPFYWNGTD
                     NIVIEVCFNNSAWAAGCTVTGDMNVGYQSAVGYYADIAAAGLCSDNTTYFTDDLPQLR
                     VIGDVPTPTAGTGSYIQFSDAVVIGNPTLFYGGDYNGPGTLTVLGAYDDNTLISDYVF
                     DEYFDGKISVEDIKKHDNYKRYSISEMSEFVEKYRHLPTIEGRDAWEEKGGFSLGELL
                     TQLWVTAENQAIYISELNTDLKLVEQDIINNQDNIRASYNQALNRINSDALLSEEVKA
                     MAVQSINDRLRLLELLINAHKP"
     gene            12956..14587
                     /locus_tag="PLM90_05815"
     CDS             12956..14587
                     /locus_tag="PLM90_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88920.1"
                     /translation="MHGQLYVLGNGTNKEVYVDGTLVGEPALYINGYLNNNGGEVYND
                     AGEIELTGNWENYPGVAGSYESNGIERFTGAVNQIVKGTMNGTTGNINQFYNLRVDKT
                     AAGQFVSLQTNANVNPSGTVNFESNGIIRTDISSHGNNGSLYAYELYVRNTAVASMIG
                     YATSGTNKYIEGRLRRQVSGSGDYYFPIGIQSTFLDGEEPFHINFASAPAANILSYVQ
                     PGTIDLLGTVMFCDVGTDPDPALGTTILDNPGSPPDGILDRLETNCQYSVEWVATASL
                     AGPYDYTITVEPGSVLQAECPFYSATWLGELKFTAKDGIPNNAALASPAPFFTPGYMT
                     CPNLFTIAGLSSFSVFRVHGITDGALVVLPIELLDFTGQTESLGNRLFWTTENETNND
                     YFVVESSADAASFKQIGIVDGAGTSTNRLSYNFLDEHPVANTTYYRLRTVDFAGAIGY
                     SNTISLTRMSNEQLSVSPVPSMSSVTVVFAADASQEAIFTITGMDGKVMLNKKWNLKP
                     GQNEQSVDITSWMPGTYVVQVDGVNAPLRTKLIKQ"
     gene            14658..15944
                     /locus_tag="PLM90_05820"
     CDS             14658..15944
                     /locus_tag="PLM90_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMZ88921.1"
                     /translation="MVSEILLILLLVLINGLLAMSEIAMVSVKKVRLESAAKRGDIAA
                     KRALQLHNEPAKFLSTVQIGITLISILTGIFSGATIKLALAEDLAKIPVIEPYADTTA
                     LVIVVIAITALSLIVGELVPKRIGMRFPEPIAKAVAGPMLLLSAITKPFVWLLTKASD
                     GIIRILRLKASSEDQVTEEEIKAIIQEGTQTGAIDEIEQDIVERVFHLGDRRIASLMT
                     HRNDIIWLDVSEPIERVYEDIQSEVHSIYPVCDGEFDKVVGIVYIKDLFIARTKKEIT
                     SLRDLIKPVLFLPENNTAYDVLAKFKESKIHYALVVDEYGSSQGLVTINDILEALVGE
                     FDELNLPDYSIIEREDGSYLVDAALPFYDFLKYFDMEEEFEMLDELEYNTLAGFILHQ
                     LEHIPKEGERLDWKQYEFEIVDMDATRIDKVMFTLK"
     gene            15950..16798
                     /locus_tag="PLM90_05825"
     CDS             15950..16798
                     /locus_tag="PLM90_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88922.1"
                     /translation="MILIADSGSSKTDWALLHPVNGDVLPLTTKGLNPIVLPVDALIS
                     IMSANEHLLECATDVSAVYFYGAGCTGDVSIGNMHAALASIFKSAKIAVFSDMTGAVH
                     AVADNKPCIVSILGTGANSCLFNGKSISGNDFSLGYVLGDEGSGTWFGKRLIRDHWYC
                     WMPESIAAEFEKQYALTREEILKQTYRSPAPNAWLASFAPFIVQHRQHEYMQEVIFEG
                     LTDFFDAYINRFENSNALPLHFIGSLSCALEPEIRNVAEENALSCGKFIQSPISGLIQ
                     FYQSSH"
     gene            16807..18456
                     /gene="pgi"
                     /locus_tag="PLM90_05830"
     CDS             16807..18456
                     /gene="pgi"
                     /locus_tag="PLM90_05830"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HMZ88923.1"
                     /translation="MIPSESFRDSIIYKALTDDAERLKQMHLRQLFQDEERFASMSIF
                     WDELLFDFSKNRIDSKAWKNLHRLAEEMQLTEAFEGMLRGDKINVTEGRPVLHTALRN
                     FSDSEVLVNGKDVMPDVREVLQKMKIFSDQIISGEKRGYTGKQFTDVVNIGIGGSDLG
                     PVMVCEALKHYHVPHLKVHFVSNVDGSHITETIKNLNPETTLFLIASKTFTTQETMAN
                     AHTEKQWFLHTAQHEQHVAAHFVALSTAEKEVTAFGIDRENMFVFWDWVGGRYSLWSA
                     IGLSICLAIGYELFEDLLYGAHQVDNLTKSVEPEHNIPLTMALLGVWYNEWFDAHTHA
                     VIPYDQYLHRFTAFLQQADMESNGKSVQRNGKRVDYATGPVVWGEPGTNGQHAFFQLM
                     HQGTQLIPADFIVAATPLHNIMEQHHMLLSNCLAQTEALMNGKTEEEVRTELRQKGMD
                     NETINKHLPYRVFEGNRPTNTIVIDQLTPYNLGRLIAIYEHKIFYQGILWNVFSFDQW
                     GVELGKKLAAEILPEWTSKETLRHHDSSTGGLIQYIRNLLG"
     gene            complement(18605..>18842)
                     /locus_tag="PLM90_05835"
     CDS             complement(18605..>18842)
                     /locus_tag="PLM90_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88924.1"
                     /translation="YQVSGIKYQVSSIRYQVSGIKYQVSGIKYQVSGIKYQVSGIRYQ
                     VKKFTTYDSRLTNHESRFTILVSRARHASPLAGI"
BASE COUNT         5560 a         3819 c         4126 g         5337 t
ORIGIN      
        1 aacttgccgg ttatccaaaa tcagaactgc cgggagtttg gatggaaatt accaaggatg
       61 caaaagatgg atataatatt cattttaata caactaattt tcaatttgct ccggacaagg
      121 taaataccga gcatgttatg ggtgagggta gcatcaacct cttcatcaac ggcgataagg
      181 ttgccagagt ttatggagag tggtatcata tcgacattgc cgccccggga acttacgaag
      241 tcgtggctac gttgaactcc aacgaactga gcgattactg tatacgagga actccaattt
      301 cagcacgcga aaaaattaca gtcagcagat aacagccatt gtgcaaaagt cctgcttacg
      361 cctttctatc ggttcattac atttgctgcc atgacagaag tggatttatt cataccttgc
      421 tttatcgatc agatgttccc tgaaacaggg tttaacatgg tcaaagtgct tgaaaaagca
      481 ggctgcaggg tgcattataa tccgaatcag acatgttgcg ggcaacccgc cttcaacgcc
      541 ggctattgga aggaaatgga ggatgtaggg aagaaatttc ttgcggattt cgcaaattct
      601 gatcgttatg ttgtaggtcc atcaggctcc tgcgttggct ttgtgcgcaa tttttatggc
      661 gaactttatg ccaacacgcc ggtgcacaat tatcacaaaa accttaagaa aaacctcttc
      721 gagttctccg aatttttggt cgacgtgctc aaagtggaag attttggagg caagtttcct
      781 gcaaaagtga cttatcacga tgcttgtggc gccctccgtg aatgtaagat aaaggaagct
      841 cctcgtaaac tcatgagtca tatcgaagga ctggaattgg ttgaaatgca attccctgaa
      901 acgtgctgcg gttttggagg cactttcgcc gttaaatttg aacccatcag cacaggtatg
      961 gccgacagta aggtaaatga cgctatcgct tccggtgccg aatatattgt gtctaccgac
     1021 tactcctgcc tcatgcacat tcaggcgtat atagaaaagc aaaatcgcaa tatcaaagtg
     1081 ttgcatattg cagacctgct ggcaatgagc attgcctgac gacaaaatag cggctaaaaa
     1141 atggccggta tgatttgagc attatggacg aaataaatca aatagcatca gacatcaggc
     1201 gatatcagga aaatggcatg cgtttattta caagttcatc tttccagaca cattctactg
     1261 tattgttgca tatcatttcc cgtattgatc ctactatccc cgttttcttt ttaaataccg
     1321 gatttcattt tcctgagact attcagtaca aaaatgaaat cagtcaacag ttcggattaa
     1381 acaccatcga ccttcgcgct gcaacgccta aattaatgca ggttgatgac cacggtagat
     1441 ttctgtttac ttcagaccct gattattgct gctatctgaa taaaatacaa ccactcgaac
     1501 ccatgctcga tacctatgat gtatggataa atggtgttcg tgccgatcaa aatgcaaaca
     1561 gagcaacatt taaaaaagaa gctcctaccg cacacagagc tgtcagatat catcctatgt
     1621 tgggatggtc gggtaaaatg atatatgcct atatcaggga acatcaaatt ccacatcatc
     1681 cgcttgatgc caaaggatat accagcatag gttgtgagcc atgcacaaga aaattaagtc
     1741 ccgaaattat ggccgatgag cggcaagcca ggtggtatgg tatgtcgaaa acagaatgtg
     1801 ggttacacac tgaattaata aagaaatgaa aatcctgatc accggcggag ccggatatat
     1861 tggttacgca ttggcacaac gattatctga aaatgataag gtaacggaaa ttatattata
     1921 tgataatctg agtcgacaca atgaaatcat tttctttgga aatgcaatcg gcaatggcaa
     1981 attaaaattg atcaaagctg acatccttga tgcgcgtaaa ttgcgcaatg caatgaaggg
     2041 tgttgatgtt gtatatcacc ttgctgcaaa agtacttaca ccttttgcaa atcaggaccc
     2101 acattttttt gagcagatca atcactgggg tacgagtgaa ctcgtagaag ttattgaaaa
     2161 atctgaggtt aagcatttta tttatttgtc cagtgcttct gtttacgacc gtgtttctga
     2221 accggctaca gaagatacca tccccacacc taatacttac tacggaataa gtaaacttcg
     2281 gggagaagat catattaaac gcatccctgc ctcaacgaaa acacatatta tcagaggtgg
     2341 caacgtatac ggttattcgc cctccatgcg ttttgatgcc gtgatcaata attttttgtt
     2401 tcaggcaaat ttcgagcata aactttccat acacggaacc ggagcacaaa cacgcgcatt
     2461 tattcatatc gataatgcat cagatgcgct ggctcaaata ccgttcagtg aattgccttc
     2521 cggcgtttat aatatggtca cgaaaaatct tgcggtgaat gatattgctg aggtaatttg
     2581 cacgctttat ccgcaatgcg acaggatatt cattaatcaa catttacaac tcggaagtat
     2641 tcgcgtaagc accgattcgc cattttggaa gtacattcag gttgaacaac gaagcctgat
     2701 ggaggaactg gcagatttta aagctaaatt tgcctttagc ggttgggact gatttcatct
     2761 atgcacgata ttcatgctat ccttaaaaaa cactggggtt acgatcagtt tcgcccggct
     2821 caggagcaaa ttatccgtgc tgctctcgat ggtcgcgata cgcttgcatt attgccgaca
     2881 ggtggcggaa aatctgtctg ctttcaagtg cctgcattgg caatggacgg gatatgtatt
     2941 gtggtttctc cattaattgc actgatgaaa gaccaggtgc atcacctaaa acaaagagag
     3001 atatctgcag ctgcaatttt ttcaggcatg tcgttccgcg aagtggatat gaccttagat
     3061 aaatgtattg caggcgatat taaatttctt tacgtctctc cggagcgact gcttacacca
     3121 attttccgtg aacggtttaa gcagatgaaa ttaaatctgc tcgccattga tgaggcacat
     3181 tgtatctcgc agtggggata cgatttccgt ccgccgtatt tgcaaatagc agcattacgg
     3241 gaatttttcc ccaaagtgcc ggtactcgcg cttaccgctt cggcaactcc tgaagtatgt
     3301 gaggatatca tgttgcgttt gcaattcaaa gaaaagaacc tcatcaaagg aagtttcttg
     3361 cgtaacaatt taagcttgat tgtgcgccaa caggaaaaca aagaggataa actactcgag
     3421 attttgcgtt ctgttccggg cacggcagtg atttatgtcc gcaatcgtcg caaaacaaaa
     3481 gaagtagcgc aatttctcat gcgccatcag attaatgctt catggtatca tgcaggtctt
     3541 gaccaggaaa cacgcagcaa tcggcaagat cagtggataa atggaaaatt gcgcgtaatg
     3601 gtttgcacaa atgcctttgg tatgggcata gataagtctg atgtaagggt agtggtgcac
     3661 ctcgatattc ctgaatcatt agaggcctat tatcaggaag cagggcgtgc cggtcgcgat
     3721 ggaaaacgtt cttacgcagg attgttattc gatattgcag atgtgaaagt gctggatgaa
     3781 aattatcaaa agcaatatcc ggaaatagcc ttcatccgac aggtgtacca tcagttggga
     3841 aattattgta aactcgcact tggcgccgga gaaaatgcaa ctttcgattt tgatatggtc
     3901 gcattttgcc ggcaatttca acttcctgcc gttgaaacaa gaaatgcatt gcgcatactc
     3961 gaacagcaca atgtgattta tgtaagcgat tctctattca agccatcaac catatttatt
     4021 ccggtagata aggaaacatt atatcgcttt caaatagaaa ataaacaatt tgagccggta
     4081 attaaattaa tcctgcgcac agcacccggc gtgtttgatg atcataccgc tattcaggaa
     4141 acatctatgg cctggcatct aacgattact caaccgaagc tgcaggaaat attacaatat
     4201 ttacatgatc aggaggtttt gatttatagt ccggtaaaaa caaaaccaca gataacattt
     4261 atccatgaac gcattcaggc ttccacgctt caattggatt acgccttgct ggagcaatta
     4321 aaaaatgctg cgttaaatag gcttcatgct attaaggcat atgtaaataa tcataccgat
     4381 tgtcgcagcg tcaaactatt acaatatttt ggtgaagaaa cgccttcttg caatgtttgc
     4441 gatatttgtg tagaaaaaaa caagctcgat ctcacagaaa aagagttccg tcgtattttt
     4501 gattggttga aagagaaact ggcaggtaat gcgttgccgc cggatgcact ttttcaggat
     4561 aaattacctg caagaaaaga gaaaattttg gaaacgatac ggttcctcgc tgataataag
     4621 caaatactgc acacaaaaga caatatcctc atttggcaag aatgaaacat atcgtttgca
     4681 cagttaccaa cgatctgacc tacgatcagc ggatggacag gatatgtact tcgctcacca
     4741 aagccggata ttttgtgaca ttgattgggt ttgtgaaaac agattctata gacaccttgc
     4801 aaaaacctta tgcgcaggtg cgattgcgac taatgtttaa aaaaggtaaa ctattttacc
     4861 tggaatacaa tttccgattg ttctggtatc tgctttttcg tagattcgac atctataatg
     4921 gtattgatct cgataccctg ttacccgttt ttgttgttgc aaaattgaag cgtaaaccta
     4981 ttgtgcatga tgcgcatgaa tattttaccg aattacctga aattgtagat cgtcctgtta
     5041 ttcagcgcgc ttggaagtgg ctggcgaaaa gaattttacc gcatgtgaaa aataattaca
     5101 ctgtgggtga ggctattgca acagcactgc aagattcata caaaaacgta tatcatgtaa
     5161 tcagaaatgt gccggtattg aatggaaacg cgcctataga aaaaggtgat tatattttat
     5221 atcagggtgc tctcaacaag ggtaggggac tcgaggcttt aatgcaagct atgcaaatgg
     5281 ttgatgcgcc tttatacatt gccggcgaag gcgatttgag cgataaattg cgcaatatgg
     5341 cagcaacatt gcctcacgcc aataaaattg tatttctcgg ctatatccga cctgatgaat
     5401 tgaaaacctt taccgctaaa gcaaagatcg gtatcaactt tgttgagcca ctgggattga
     5461 gttattatta ttctttgtcg aataaattct tcgattacat ccacgcaggt atccctcagg
     5521 taaccatgga tttccctgaa tacaaacggt tgaatgattt atttaacatt gcactgttaa
     5581 ttgatgagat tacccctgag aaaatagcgg cggctttgaa tacattgctc aatgatgcag
     5641 ttgtttataa taaactggca gccaacaccc aagcagcaaa acgcgaattg aactggcaga
     5701 gggaagaaga aaaattggta aaattgtatc gtcaatttga aaagtaaaca tctgcatatc
     5761 atttctttca atgtgccata tccaccggat tatggtggtg tgatagatgt gtttaataaa
     5821 atcaaagcat tgcatgcaga aggaattaaa attcatctcc attgctttga atatggtcga
     5881 aatgaagcgc ctgaattaaa tgctttttgc gaacatgttt attattaccc gcgtttgccc
     5941 atgtggcgag gattcttttc gcgcaagcca tttttaatcg cgtcacgcaa atctaatgca
     6001 ctgagaaaca ggttactcgc cgatgagcat ccaattctgt ttgaaggatt gcatgcgtgc
     6061 tatctgctgg atgatcctgc ttttcaaaat agaataaaac ttgttcgtat gcacaacaac
     6121 gagccggttt attataagac actcgcgaac agagaaaacc gtatgttcaa aaaaacatat
     6181 tttctcacgg aggcttcaag gttgtccaga tttgaatccg tgcttagcca cgcgcaacat
     6241 atactttgta tttcgcctta cgaagctgat cattatgaaa ttaggtttcc gcaaactact
     6301 tatatttctg ctttccatgg acatgaagca atacattcaa aacccggaaa aggaagcttt
     6361 gtattgtatc atggtaacct gagcgtaaat gagaacgacg aagccgtgaa atttatattg
     6421 aaagaaatat ggaaggaatt gaatgtgcca cttgtaattg caggctcagc tccttctgaa
     6481 attcttgtcc ggttaataca ggaatatccg caggtgaaat tagtgcaaga tcccgatcaa
     6541 aataccttgg aagaattgat ccgaaacgca catatccatt tgttgccggc aatgcaacag
     6601 accggtatta aattgaagtt gttgaatgcg atatttaatg gaagattctg catcgccaac
     6661 agaaatatga tcgagcatac tggattggaa caatattgcc tttgttttaa tcatgccgat
     6721 gaagcaaaag cagcaataaa tgcctatatg cagcaagaat ttactgctga agatgttatt
     6781 ttcagacagc cggtagtcga caccttcagc gatagcgcct ctgctaaaaa aattatcagc
     6841 tatttaccgg aacatccgta atcaattttc cgtttgccgt ttttaaaatg cgagcaggga
     6901 atttttccag tatattgtaa ttgtgtgttg ccatcagaat cgtcgtgtta tattccttat
     6961 tcaggaaacg caataattgc ataatatcat cacttgtttc aggatccaga tttcctgtag
     7021 gttcatctgc tacaatagca accggatcgt taatgatggc tctggctatt accacgcgtt
     7081 gttgttcacc acccgataat tcatgcggca ttttaaatcc cttcgactgc aaccctacgc
     7141 gctttaatgc ttcatcggat tttaatttaa taagcgatct gtcagttgtg cctgttgcgc
     7201 gcaatacaaa ttccagatta tcatctactg acctatccat cagcaattga aaatcctgaa
     7261 aaatgattcc tagtttcctg cgcaaaaatg gtacagtctt ccatgtgatt ttagcaagat
     7321 caaaaccggc aacttcggca ctgccgctac gcaaagaaag atcgccgtaa attgttttga
     7381 gcagactgga tttcccggta ccggttttac ctatcaagta cacaaattct ccatgcggta
     7441 tgctgaaggt gacatcactc aacaccaaag ttttaccctg aaaaatgctt acaccggaaa
     7501 agtgaatatc gttaccgctt gttgtttcca tgtggcgaag ttacctgctg tcatgccaag
     7561 gtaaatatcc acttatgcac aatagatttc ggcctcgctc ttgtaacttt gcaccatggc
     7621 attatcgaat gatttgctgc tgcgtgtcgc caatcgtcaa tcagtagaac gcccgccggt
     7681 atggttaatg cggcaggcag gaagaatact ccccgaatac cgtgcactga gagaaaaggt
     7741 acaagggttt aaaaacctgg tagaaaaccc cgaattagtt tgcgaagtaa cccttcaacc
     7801 ggttcgcgtc cttggtgtag atgccgccat cctgttttcc gatattcttg tggtgcctga
     7861 ggccatggga cttccatacc aaatggtgga atccaaaggc ccatggtttg aaaatacaat
     7921 tgattcaccc tcagacctgt caaaccttca aatcgttgat cctgaggctt ctttaggctt
     7981 cgttttggat ggagttaaat tgatcaaagg ggaattgaac gacagcatac cccttattgg
     8041 cttcgcagga gccccgtgga cgatctttgt ctacatggta gaggggagag gctctaaaac
     8101 ctttagtaag gccaaggctt ttctttaccg gcatcctgag gcctcgcaca agcttctcag
     8161 catgatcacc gaggctacca ttgcatattt gaaagctcag atcaaggccg gcgtgaacct
     8221 gatacaactc ttcgactcct gggcaggaat tcttagtcct gagcaatatg ctgcgttcag
     8281 tatgccatat atcagcagaa tttgcaatgc catcgatgaa gtgccgaaaa cggtgttccc
     8341 caaaggagct ttcttcgcgc gtcaggcatt gtcggaaatc aattgcaatg taatcggact
     8401 tgattggaat atggacattg cagaaacaaa gcaaattgtc aatggcagaa aggccttgca
     8461 aggcaatctg gacccctgtg tgctctatgc cgaggcatcg gtagtaaaaa gtgagaccct
     8521 caaaatgctg cgtgcattcg ggaaagatca tatcgccaac ttaggacatg gagtttatcc
     8581 ggacacacca ttagacaatg taaaatgctt tgtcgatacc gttaaagaat ttgcatacac
     8641 cggcgaataa attctattat tttcatgtat acacatggtt gtgcaaaaaa aatgtaaata
     8701 cgggcttgca tagtcctatg cagaatggta tgtttgttta ggagaacata catcaaatga
     8761 ggacttttta cccgaaattc ctttttattg tgacatttct gggcctgacg gttttcgccg
     8821 aagggcaggg gtacgtaggc attggaacta ctactccggc tgaaaaacta gatgtgaacg
     8881 gtgccataat agttagaggc gatgcgaccg ccgctacccc cgttgccggc acagtcagat
     8941 ggaacagcac cgacggaatg cacgatggaa ggacatcagc cggcgaatgg cgccgactag
     9001 aaaataactt ttccttactt tcaggcacct attataacgt gagttgcgga gctacgctta
     9061 cgactaccgt tggtgccgga accagcacaa gtgccaccac gtacgacacg ccttttggaa
     9121 cgcaatacgc cgataacaag cgcgactacc tgtattttgc ttcggaattg actgctgcag
     9181 gttactgcgc aggaaccatc actgctttgg gctttgaaat tgtatctctg ggaacaagca
     9241 ccatcaacaa cttccagata ggtatgcaat ctacagcatt aactgctatt tcgggtacta
     9301 catttgcagg tggttataca attgtttacg gaggacccgg aaccacaatt tcccttacag
     9361 ccggtgtaaa ctattttacc cttactacgc cctttacttg ggatggaaca agcaatattt
     9421 tgattcaggt ttgctttaat aatggaggtg tcggatattc tggaactaac tcatcggtaa
     9481 aatgtaatac caccggagta actgtaaaaa cacggtacta ttatgctact ctaccatcag
     9541 atgggtgtac aatggctaca ggaacatcta ctgctgtatc acgtccaaat ttgtatgtaa
     9601 ccggtaagac caccggacct atcggcacat caggcaatta tttatattca gcattaccat
     9661 gggttgttgg aacgcctgcc atacctgctc cttacgtgca tcatggcgat ggcaccgtta
     9721 ccgcacaatc gctctacgat gacaatactt tgttaagtga tcatgtattc gattattatt
     9781 tcgatggtaa ggtcgcggat caggatttag cactccattc cgattataaa atggcatctc
     9841 ttgaagagat gatagctttt atggaagagc agcgacatct ccccaccatt caaggtcgca
     9901 gtgcatggga acagaatggc aaattctccg tcggccagct tagcaccgaa ctttgggaaa
     9961 catctgaaac acagtcgctc tatattttgg aattaaacaa gagaatcgaa gcattgaagg
    10021 ccaaatctga ttctctcgaa cccactaaaa agtaacaggc atgacaacac acttacgtgc
    10081 ttttattttt gctctgcttt tacttccggc aataactgtg ttcggacaag gagaggttgg
    10141 ctttggcaca gacactcctg ctgaaaaagc cgatatcaat ggcgcgatga ttattcgtgg
    10201 cgctgcagtt gctgcaacac ccattcttgg cacaatacga tggaacagta ccgacggtat
    10261 gcacgacggt cgcgaaactc cgggagtctg gacaagattg gaaaatgatg aattcgaata
    10321 taacggcgac tacactgcca tggtttgcgg aactacttca accacttcag caggaaccgc
    10381 taccggaaca agtactactg catcagaaac accatttgct acggcttata gcgataaacg
    10441 aagccaatac ttatattatg gaactgagat tgcgctcggc ggactttgtg ccggttatat
    10501 cacccaaata ggctttcagg taagtgtgct tggcgctccg gcagctttga acggtttaac
    10561 cgttagtatg aaaatgacaa ctactacatc gctttcaggc ttaacttggg ataccggact
    10621 taccacctat tataatggaa attacacctt aggccccgga cctaattata tttcactaac
    10681 atcaggagca tatccttccg gattttattg ggatggttat tctaatatcg aattggaatt
    10741 atgttacgat aacgtttcaa gttcatttaa taccactgta gaaatttctt ccggattagc
    10801 atatacggca actcgtacac attacgccag tccgggtgtc ggatgcgcac ttacagcatc
    10861 agtaactgag gcaaaacacc ccgtattata cctcacagga acaggtgttg ggccaactac
    10921 cggagtgggt gattatttgc aatttaattt gccaattatg attggctcac ccgttctacc
    10981 cgcaccttat gtgcaccatg gtcccgggtc aattacggct gaagcaattt atgatgaaaa
    11041 cgtaattatc agcgattatg tgtttgataa ttatttcgat ggtgaaatgt cggaaacaga
    11101 taaagtaaaa catgccgatt acacaatgcg cagtattgat gaaatgactg aatacatgcg
    11161 cattcaccga cacctgccaa ctatcagagg aagaaatgat tgggaagaaa atggaaaatt
    11221 cagcaccggt gagcttgctt cagaattatg ggtgacttat gaaacacatg ccctttatct
    11281 caaagaatta catgaacgca cacttgttgc agaacaatta ttaatgacct ccgacgagat
    11341 attgaaagat gcttatctcg cagaaatatt ccgattgaat caagataaca ccttaacaga
    11401 atcggtcaag tcagaaagaa tcgcattgtt gaaccaaaaa attgaggaac tgaaccatgc
    11461 gaagtaatag caagataatg aaagggattt atttagcagc gtttttatcg gttgcctctc
    11521 ttagtgcatt tagtcaagga tacaccggaa taggtactaa tactccgcaa gaacgtctag
    11581 atgttaatgg ggcaataatc gtcaggtctg atgctgtttc acctacacct gttcccggaa
    11641 caattcagta taatactacc gatggttatc atgagggtta tacaagtgcc ggaacctgga
    11701 taaaattgga aaatgaaaat accattggtt acggcgatta tatcactgtt gtagaaacgt
    11761 gtacttctcc tgccactgtt ggaagcaatg aatatacaac gccttcaaca ggatatttta
    11821 atcccaacta tgaagaaaat ccgttttcta cttggtggat ggatgacaga acacagttat
    11881 tatataaggc ttcgcaatta accgctgcag gcatctgtgc gggaaatatc aatgccatcg
    11941 gttttaacgt cgtgactccc ggagcatatg ccatgacaag tcttactatt aagatgaagt
    12001 cgaccaatat tactactttg acagcctacg aaactgcatt gtcaactgtt tattccgcgg
    12061 gctcttatac acctgttgtt ggcgcaaatg attttacact tacctctcca ttttattgga
    12121 atggaactga caatattgta attgaagtat gttttaataa ctcagcatgg gcagcaggtt
    12181 gtacagttac cggcgatatg aacgttggat atcagtcggc tgtcggttat tacgccgata
    12241 tcgcagctgc aggattgtgt tcagataata ctacctattt tacagatgat cttccgcaat
    12301 tgcgcgtcat tggcgatgtg cctacaccta ctgcaggaac aggttcttat attcaattca
    12361 gtgatgccgt tgtaattggt aatccaacat tattttatgg tggtgattat aatggcccgg
    12421 gaacattaac agtgctcggt gcctatgatg ataatacctt gatctctgat tatgtgtttg
    12481 atgaatattt cgatggaaaa atatctgtcg aggatattaa aaaacatgat aattataagc
    12541 gatactctat ttcggagatg agtgaatttg ttgaaaaata tcgccatctc cctaccattg
    12601 aaggacgaga tgcatgggag gaaaaaggcg gtttctcact cggagaattg cttacacaac
    12661 tatgggtaac ggcagaaaat caggctattt atatttctga attaaatacc gatttgaaat
    12721 tggttgaaca ggatatcatc aataatcagg ataatattcg cgcttcatac aatcaagctt
    12781 taaatcgcat taattccgat gcattattgt cggaagaagt aaaagcaatg gccgttcaat
    12841 ccatcaacga tagattgcgc ttgctcgaat tattaattaa cgcacacaaa ccataacgct
    12901 caccgtgaaa agacacatac tcatatcatc tatgtgcctg ttgacagcga tcaatatgca
    12961 cgggcaatta tacgtattgg gcaatggcac aaacaaagaa gtgtatgtcg acggcaccct
    13021 cgttggcgaa ccggctttgt atattaatgg atatctgaac aataatggtg gcgaagtata
    13081 taacgatgcc ggtgaaatag aacttaccgg caactgggaa aattatcccg gcgttgcagg
    13141 ctcctatgaa tctaatggta tcgaacgctt taccggcgct gtgaatcaga tcgtaaaagg
    13201 aaccatgaac ggtaccacag gtaatatcaa ccaattttat aacctacgtg tcgataaaac
    13261 tgctgcaggt caatttgttt ccctgcaaac aaatgcaaat gtcaatcctt caggcactgt
    13321 aaactttgaa tctaacggaa taattcgcac agatatcagt tcacatggaa ataacggttc
    13381 tctgtatgct tatgaattat atgtgcgcaa tacggcagta gcttccatga ttggttatgc
    13441 tacctcagga acaaataaat atatcgaagg tcgcctgcgt cgtcaggtga gtggctcagg
    13501 agattattat ttcccgattg gcatccagtc gacattttta gatggtgaag aaccttttca
    13561 tatcaacttt gccagcgctc cggcagcaaa tatcctgtcg tatgtgcagc ccggtacgat
    13621 tgatctgtta ggaacagtca tgttttgcga cgtgggaact gacccagatc ctgctttggg
    13681 aactaccatt cttgataacc cgggatcacc tccggatggt atcctcgacc gactggaaac
    13741 aaattgtcag tattctgttg aatgggtcgc tacggcaagc ttagcgggtc cttatgatta
    13801 tacgatcaca gttgaacccg gttctgtgtt gcaggctgaa tgtccattct attctgctac
    13861 ctggttgggt gaattgaaat ttacagcgaa agacggaatt ccaaataatg cagcattggc
    13921 atcccctgca ccatttttca caccgggcta tatgacatgc ccgaacttgt ttacgattgc
    13981 gggtttatcc agcttttccg tattccgtgt gcatggtatt actgatggtg ctttggttgt
    14041 tttacctatc gaattattgg attttaccgg acagacagaa tcgctcggca atagattgtt
    14101 ctggactact gaaaatgaaa ctaacaacga ttattttgtg gtagaaagtt ctgccgatgc
    14161 ggctagtttc aaacaaatcg gaatcgttga tggagcagga acttctacta atcggttgtc
    14221 ttacaatttc cttgatgagc atcctgtggc gaataccact tattatcgtc tgcgcaccgt
    14281 agattttgcc ggtgcaatag gttatagtaa tacgatctca ttaacacgca tgtcaaatga
    14341 gcagttaagt gtttcacctg tgccatcaat gagttcagtt accgttgttt ttgcagctga
    14401 tgcatcacaa gaggctatat ttactatcac cggtatggat ggcaaggtaa tgctcaataa
    14461 gaagtggaat ctcaaaccgg gccaaaatga gcagtctgtc gacattactt catggatgcc
    14521 gggcacctat gtggttcagg ttgatggcgt caatgctcct ttgcgcacta aattgatcaa
    14581 acaataagct gtcgaaggcg ctttacagta tccttacaag tcagtaatgg ttacaatcta
    14641 tatctttacc accggatatg gtaagtgaga tattattgat attgctgtta gtcttgataa
    14701 acggattact cgccatgagc gaaattgcca tggtgagtgt taagaaagtt cgcctggaat
    14761 cggcggctaa aagaggcgat attgccgcta aacgagcgct acaactacat aatgaaccgg
    14821 ctaaatttct cagcaccgtt cagattggga taaccctcat cagcatttta acgggtattt
    14881 tttcaggcgc aacaatcaaa ttggcgttgg cagaggacct tgccaaaatt cccgttattg
    14941 aaccatacgc agatactacg gcattggtta tagtagtaat tgccattacg gcattatcat
    15001 tgattgtggg ggagctggtg ccaaaacgaa taggcatgcg attccccgaa ccaattgcaa
    15061 aggcggttgc cggaccgatg ttactgttat cggcaattac aaaacctttt gtttggctgc
    15121 tgacaaaagc cagtgatgga attatcagaa ttctgcgcct aaaggcttcg agtgaagatc
    15181 aggtgactga ggaagaaatt aaagccatca ttcaggaagg aacgcaaaca ggggcaatcg
    15241 acgaaataga gcaggatata gtggagcgcg tttttcacct gggcgacagg cgtattgcca
    15301 gcctcatgac ccatcgcaat gatattatct ggcttgatgt aagtgaaccc atcgaaaggg
    15361 tttacgagga tattcaaagt gaggtgcata gtatttatcc ggtttgtgat ggagagttcg
    15421 ataaggttgt aggtatcgtg tatattaaag acttgtttat tgcaagaacc aagaaagaga
    15481 ttacaagcct gcgcgatctt attaaaccgg tattgtttct tcctgaaaat aatacggctt
    15541 atgatgtgct tgctaaattc aaggaatcga aaattcatta tgcattggtg gttgacgaat
    15601 atggatcgtc gcagggactt gttaccatca atgatatcct cgaagcactg gtaggtgaat
    15661 ttgatgaatt gaatcttcct gattattcaa taatcgaaag agaagatggt tcttatcttg
    15721 ttgatgcggc tcttcctttt tacgacttct tgaaatattt cgacatggaa gaggaattcg
    15781 aaatgctgga tgaattagag tataatacgt tggcaggatt tattcttcat caattggaac
    15841 atattcctaa agaaggcgaa cgcctcgatt ggaagcaata tgaatttgaa attgtagata
    15901 tggatgccac acgcatcgat aaggtaatgt ttaccctcaa ataattttca tgattctgat
    15961 agccgatagc ggatcttcca aaacagattg ggccttgctg catccggtga acggcgacgt
    16021 gcttccattg actacaaagg gattgaatcc gattgtgctt ccggtcgacg cgttaataag
    16081 tatcatgtcc gcaaatgagc acttattgga atgtgctacg gatgtcagcg ctgtttattt
    16141 ttatggcgct ggatgtacag gtgatgtatc aattggaaat atgcatgcag cattggcaag
    16201 tatattcaaa tccgccaaaa ttgccgtgtt ctccgatatg accggtgccg tgcatgctgt
    16261 tgcagataat aaaccttgta ttgtttcaat tttaggtact ggtgcaaata gttgtttgtt
    16321 taacggtaaa tctatttcag gtaatgattt cagcctgggt tatgtgctgg gcgatgaggg
    16381 ttcaggcaca tggtttggca agcgtttaat tcgtgatcac tggtactgtt ggatgccgga
    16441 aagcattgct gcggagtttg aaaaacaata tgcattaaca agagaagaaa tattaaaaca
    16501 aacatatcgc tctccggcac caaatgcatg gctggcttcg tttgcaccat ttattgtgca
    16561 acacaggcaa catgaatata tgcaggaagt gattttcgaa gggttaacgg atttctttga
    16621 tgcctacatc aacaggtttg aaaactcaaa tgccttgccg ctacatttta tcggcagcct
    16681 gtcatgtgca ctggaacctg aaataagaaa tgtggctgaa gaaaatgcac tttcttgcgg
    16741 caaattcatc cagtcgccga tcagtggatt aatacaattt taccaatcat ctcattaatc
    16801 tttgttatga ttccatcaga aagtttcaga gatagtataa tttataaagc gcttaccgat
    16861 gatgccgaac gcttgaaaca aatgcacctc aggcaattgt ttcaagatga agaacgcttc
    16921 gcttccatgt ccattttttg ggatgagctg ttatttgatt tttctaaaaa cagaatcgac
    16981 agtaaagcat ggaaaaattt acatcgctta gccgaggaaa tgcagcttac cgaagctttt
    17041 gaaggtatgc ttcgcggaga taaaataaat gtaactgagg gtcgaccggt attgcatact
    17101 gcattacgca atttttctga cagcgaagta ttggtgaacg gaaaagacgt tatgcctgat
    17161 gtgcgagagg tgttgcaaaa aatgaaaatc ttttcagatc agattatttc gggtgaaaag
    17221 aggggatata ccggtaaaca atttacagat gtagtaaata ttggtatcgg tggctcagat
    17281 cttggtccgg taatggtttg cgaagcacta aaacattatc atgttccgca tcttaaagtg
    17341 catttcgttt caaacgtcga tggatcacat attacagaga caataaaaaa tctgaatcct
    17401 gaaacaacct tgtttcttat tgcctccaaa acttttacta cacaagaaac catggccaat
    17461 gcgcataccg aaaagcaatg gttcctgcat actgcacaac atgagcaaca tgtggctgca
    17521 cattttgttg ccctgtcgac tgcggaaaaa gaagttactg cattcggaat cgacagagaa
    17581 aatatgtttg ttttttggga ttgggtagga gggaggtatt cactctggtc ggctatcggt
    17641 ttgtcgattt gtcttgccat cggttatgag ttgtttgaag atttattgta tggtgctcat
    17701 caggtcgaca atcttacgaa gtcagtagaa ccggagcata atattccgtt gaccatggcc
    17761 ttattgggtg tttggtataa cgaatggttt gatgcacata cccatgcggt gattccttac
    17821 gatcaatacc tgcatcgctt cacggcattt ttgcaacagg cggacatgga gagcaatggc
    17881 aaatcggttc aacgcaacgg taagcgtgtg gattatgcta cgggacctgt ggtgtggggt
    17941 gaaccaggga cgaatggcca acatgctttt tttcaactca tgcatcaagg cacacaatta
    18001 atcccggccg attttattgt tgctgcaacg cctttgcata acatcatgga acagcatcat
    18061 atgttgctga gtaattgcct ggcacaaaca gaagcattga tgaatggtaa aaccgaagaa
    18121 gaagtgcgta ctgagcttcg tcagaaaggg atggataatg aaaccatcaa taagcattta
    18181 ccatatcgtg tgtttgaagg caacaggcct actaatacaa ttgtgattga tcaacttacg
    18241 ccatataatt taggtaggtt gattgcgatt tatgaacaca aaatatttta tcagggtata
    18301 ttgtggaatg tgttcagttt cgatcaatgg ggagtggagc ttggaaaaaa attagctgct
    18361 gaaatattac ctgagtggac aagcaaagaa acattgcgcc accacgattc aagtaccggc
    18421 ggacttattc agtatatcag aaatttgttg ggttaataag tgtaaatgcc tgcagaatca
    18481 attcgccatc aaatcgtgac tcgtgagtcg tgaatcgtga gtcgtgaatc gtggatcgtg
    18541 agtggtgagt ttttttaaat acccgatacc aaaaacacat cacaagcatg cattagtcta
    18601 gctactagat acccgccaga ggcgaggcgt gcctggctct agatactaat atcgtgaatc
    18661 gtgattcgtg attcgtgagt cgtgaatcgt aagtcgtgaa tttttttacc tgatacctga
    18721 tacctgatac ttgatacttg atacctgata cctgatactt gatacctgat acctgatact
    18781 tgatacctga tacctgatac ctgatacttg atacctgata cttgatacct gatacctgat
    18841 ac
//
LOCUS       DAOQOU010000045        18534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1502_length_18534_cov_5.452135, whole genome shotgun sequence.
ACCESSION   DAOQOU010000045 DAOQOU010000000
VERSION     DAOQOU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1502_length_18534_cov_5.452135"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLM90_05840"
     CDS             complement(<1..299)
                     /locus_tag="PLM90_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HMZ88925.1"
                     /translation="MKTRNRHLIVIAGPTASGKTACAIALAKALQTEIISADARQFYR
                     EIPIGTAAPTKEELSQVPHHLVGQLSIHDAYDAGIYAEEVMTLLAQLFQKFETVI"
     gene            complement(304..1755)
                     /locus_tag="PLM90_05845"
     CDS             complement(304..1755)
                     /locus_tag="PLM90_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ88926.1"
                     /translation="MSGNLHIQSQQLQFSDAEHALFKVVAEVSATLQFPCYVVGGFVR
                     DKILGRDYKKDIDFVCVGSGIALARAVAATLPGKPSVAEFKNFGTAHFRYHGMEIEFV
                     GARKESYRHDSRKPIVEDGTLRDDQLRRDFTINALAISLNENDYGAVIDPFNGIHDME
                     DRCIRTPLDPAITFSDDPLRMLRAIRFASQLNFDIEYDTYAGIKASKDRIRIISQERI
                     TDELNKILLSPKPSIGLYYLSDSGLLEIIFPELYAMHGTDMIDGQGHKDNFFHTLEVV
                     DNISALTDDLWLRWAALLHDIAKPATKKFIPPTGWTFHGHEVVGARMAKQIFTHLKLP
                     LQQELKRVQNIVGLHMRPISLTKENVTDSAIRRLLFEAGDDLEGLLLLCHADITTKNR
                     LKIKKYHENFELVKEKLRDVEARDHIRNFQPPVTGELIMETFDIKPGKEVGQIKEFVR
                     EAILEGDIPNEYDAAYRLMLEKGLSLGLQPVKR"
     gene            complement(1771..2577)
                     /locus_tag="PLM90_05850"
     CDS             complement(1771..2577)
                     /locus_tag="PLM90_05850"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HMZ88927.1"
                     /translation="MQALIEKAWNDRSLLQDTAMQHTIKEVIAMLNNGSLRVAEKVNG
                     NWQVHEWVKKAVIMYFPIMQMETISVGPFEFHDKIPLKHNLKEAGIRVVPHGLIRYGA
                     FVARNVIVMPGYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPVQA
                     SPVIIEDNCFIGSRCIVVEGVVVEEEAVLGANVVLTQSTKIIDVTGHEPVEYRGRVPK
                     RSVVIPGSYTKKFPAGEYQVPCALIIGQRKESTDKKTSLNDALRDNSVAV"
     gene            2703..4184
                     /locus_tag="PLM90_05855"
     CDS             2703..4184
                     /locus_tag="PLM90_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HMZ88928.1"
                     /translation="MRAKLIMLLGPVLAVITGLLFYYTGNSPEVSKMAAVLVLMAYWW
                     MTEAVNIFITSLLPVVLLPLLGILSIDAVAPQYMKDVIFLYIGGFILTFAIERWNLHR
                     RFSLGILLRFGSSPEKLLIGFMVSTYFISMWLNNTSTTLMMLPVALAVIKQIHGNDDS
                     KRTGLSTAILLGISYASTIGGTATLVGTLPNMVMKSFYDEHFPAEQSLNFANWFSFAF
                     PMSLFFLAICYFVFKAMLLRNSDHKAPDLEYCKTEYRALGKMTFEEKAIAWIFLAAVV
                     LWFTLDNKTLWGLDLVGWKTYLVHWHIIPNDKFVNESFVAILMGMILFFFPSKSKKGE
                     SLVMWEDVKRLPLGILFLFGGGFALAKGVEVSGLDDWIAGNCTGLKDLPPILMILCLC
                     VITTIFSEFASNTATVSLFMAILIPIIPMLGIPPLLAMMPITIAASYSFMLPVGTPPN
                     TIVFGTEMIKVRTMLRVGLWLDLLGALVILLFTFTIGQWVFGF"
     gene            complement(4286..5740)
                     /locus_tag="PLM90_05860"
     CDS             complement(4286..5740)
                     /locus_tag="PLM90_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88929.1"
                     /translation="MKKITYPCILLIISLMMVAPAKAQLEDGSIAEDWTLTDINGTQW
                     HLYDILNQGKSVFLDFSAVWCGPCWTYHNSGNLEALYDTYGPDGTNEVMVFYIEGDGA
                     STLDELNGIGAGTQGDWVDGTPYPIILTHYGDPSYTVVSDYEIAYFPTIYRICPNRIV
                     DEVGQVSTAVLYASTDDCAVAFTNNDASIMDYTGPTVGCAEVELSITLQNMGFDPLTA
                     CTITANQDGVELLSYNWTGNLDIYEMEEVTIGSVVLDDESSNIDINITSADDNDLNNT
                     VYTTIFFHNNVNMVIHLEVKTDNYPTQTRWEIIDEESGDEVHSGGPYPTSDKNTVVVD
                     EDVTLPGNGCYTFHMFDSGGDGITGTGYYKIYDNGEVIADGDEDIAYKKSEALKVTGL
                     TEITAATVNQNLMISPNPANQSTSITVQHTGGDLFIELVDMLGQHIMTVNTSNCAAGE
                     QHYTINTSMLAEGNYIIRVQAIGNTQGSILTVTH"
     assembly_gap    5845..5944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6087..7550)
                     /locus_tag="PLM90_05865"
     CDS             complement(6087..7550)
                     /locus_tag="PLM90_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88930.1"
                     /translation="MKKLFLSLSLAALAIFGFNNAAKAQLEDGSVAPDWTLTDINGTT
                     WNLYTLLDEGYDVFLDFSAVWCGPCWAYHTGGSLEGLYDAYGPDGTNEVMVFYIEGDG
                     ASTLDELNGIGAGTQGDWVTGTPYPIVLTHAGDPSYSAVQDYDIAYFPTIYRVCQNRI
                     VDEVGQLSTAALYATISDCAAASVNVDPSILTYNGPVASCGDVPVSVTIQNMGFNELT
                     ACTITAYADGIEVASVDWTGSLNTYEIATVDVGTFTPADADVDLSVEITSADENTVNN
                     VIATTVTYEDNITQIIHLELKTDSYPTETKWEVIDESTGDVVAEGGPYTNAQKNEIVF
                     DEDIVLPSLGCFTFTCYDAYGDGITGSGYYKLYDNNGDLIQQGMENIGFEKSAALKVT
                     TAVAIEENSAINNVSLYPNPANDNINVDINLTATADVDVTIVNMYGAVVGTATSGTLT
                     AGAHSFTVNTADLANGMYFVQITGENVNNTVKFTVFH"
     gene            complement(8167..8844)
                     /locus_tag="PLM90_05870"
     CDS             complement(8167..8844)
                     /locus_tag="PLM90_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88931.1"
                     /translation="MKKILLVTLVALTSIGAQAQTAKFHDGMKKALTLMDSAKTGDDF
                     MNVANSFERIAMAEKNQWLPYYYSALARTTALFIYNDVTRNDAVLDVAEKHAMKADSL
                     SPNNSEIFVLRSMIFGGRIMVDPMSRGQMYGMQSMMLLQQAMTADPNNPRSYYIMGQS
                     LFYTPPQFGGGKEPGCKMFETALQKYSIFVPQSDLHPNWGLDQVNQMLSQCSATTDPN
                     TGKSGGN"
     gene            complement(8889..11069)
                     /locus_tag="PLM90_05875"
     CDS             complement(8889..11069)
                     /locus_tag="PLM90_05875"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ88932.1"
                     /translation="MKQAILLFIGISSITALTAQTTISGTVTDNKKNLLPGANIFLKD
                     TYDGTTSNVDGTYSFITTETGKQILSVSFVGYESFEDTLQLNGGTLSVNIVLKEAFNE
                     LNAVVITAGAFEASDEKKNTILKPLDIVTTAGAEGDIYGALETLPGASKVGNDDGLYV
                     RGGSDYETKTIIDGMVVNNPYYSTVPDVPSRGRFQPFMFKGTVFSTGGYSAEYGQALS
                     SAVILNTEDMPEYSASGLSVAPIFLGGFHTQKFKNEKTALGGGINYTNLGLFDNVFKP
                     TTFDWINSVESVDGSLFFRQKTSKTGILKLYAQKESSNMALRAPDIDSLPATDDVYLG
                     NNYNYINASWREILTKKWGVQAGVSYSKNNDDILVDGFNVGRSDQNAQGKVTFNNEIT
                     EKIKLRYGGEFQHITYDQFAGDLEFDATDNFTAAFTESDIFITNDLAGRIGVRAEYSE
                     ILDDVNVAPRISLAYKTGKNAQVSLAYGDFYQTPQPNYLYTQALYPLQYEKATHYIAN
                     YQYITNDRTFRIEGYYKTYDQLVTLTTDSLNPFVPITANNGSGYARGIDLFYRDKKTI
                     DYADFWISYSFLDTKRKFLDYPEEVTPTFAAQHILSLVYKHYFPGINTQVSGTYRYIS
                     GWPYYNPNNPEFLGDVSPDLHNVALSASVLQSLWGNFTVFFISWDNTFGFDQIYGYNY
                     SADGSQFLVNQAPVKSTVFFGIFMSILYGKDKNVDDIDIDKSNK"
     gene            11237..11992
                     /locus_tag="PLM90_05880"
     CDS             11237..11992
                     /locus_tag="PLM90_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ88933.1"
                     /translation="MAIVMITGASSGFGAATAEIFAQNGYRLILNARRIERLETLKRM
                     LSETYSADVLLLPFDVRDKDAVATAMDTIPASWKQIDVLINNAGLAAGRDLFQDGLVD
                     DWEAMIDTNVKGLLYVTKHAVSGMKERKSGHIINVSSLAGQEVYPMGNVYCATKHAVT
                     ALSKGLRMDLLPYHIKVTNISPGLAETEFSMVRFKGDEAKAKAVYEGFDPLQAKDIAD
                     VIYFAASRPQHMNLEEITILPTAQSDSRTVVRG"
     gene            complement(12277..12792)
                     /locus_tag="PLM90_05885"
     CDS             complement(12277..12792)
                     /locus_tag="PLM90_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ88934.1"
                     /translation="METLHTERLTLSPCTIDDAAFLLALLNSPGWLKYIGDRNVHTLE
                     EARSYMQNRIMKAYEKDDYGMWLVRDTTTGAAMGLCGIVVRDTLPHPDLGFAFLPEFQ
                     GKGFAFEAAQALMEFAIGYTDIDTMLAIATPDNTHSIKLLTKLGMSFESEFTDPHTKD
                     LLVRYSINLNI"
     gene            complement(12798..14510)
                     /locus_tag="PLM90_05890"
     CDS             complement(12798..14510)
                     /locus_tag="PLM90_05890"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88935.1"
                     /translation="MSSLSFHVFMDNPHTHTFTIKMHIPAMGKDVLSLALPVWTPGSY
                     LVREYSKNINRLYCEDKNVGINKTAKNTWEITTNNQAIDIIYELYAFEESVRTCWLDS
                     ERASIIPAGMFLFIEDTPLPVNISFHPHHAFKDIATSLPMVNNDPWVRFAADADTLYD
                     SPIEIGNHITQHFNAAGVEHTLVISGTGNYHIPSMITDMQAVIETEVNIFKHHPCKSY
                     LIILNTSNTLRGGLEHLHSTSLIFKRLMFADRSNYQEFISLFAHEYFHLWNVKRLRPK
                     ALGPFNYITENYTTSLWIAEGFTSYYDDLIVYRTGLYAEEEYLTIVEKNINLAENAPG
                     KKIMSVAEASFDAWIKYYRQNENSENSQVNYYVRGGVLATLLDILIIHHSDGKHNLDN
                     VMRTAYERFYVNRDSGYTEDEFKAVIEEFAGISMNDFYRQYVFGTSQIAFNDILCHVG
                     LQLENTLENTIESDLGITINNNNIITAVRRNSAGEQAGIYVTDEIIAINGYRYSNGLL
                     ASLTADAKTGDKLEIILSKSGRMHTVSATIQITDKVNYRLKAMDDANDSQRAHYAKWL
                     LKNK"
     gene            complement(14550..16754)
                     /locus_tag="PLM90_05895"
     CDS             complement(14550..16754)
                     /locus_tag="PLM90_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ88936.1"
                     /translation="MRQILTIFLLCLSQLLFAQDVQWASEVVKFSTEYSRTQFSAKQA
                     LGAPNKLPASGESTVAWAPSTPDNPAGEFIQVRFAQPQYVQQIGIGESNAPGAVKEVV
                     LISTEGKKYTVYTNDTIKPAYNTGSRVFRIFIPRTDYLVKEVKVSLNTKVIAGMNQID
                     CIGISDSREPIKAKINELAYAEKLPPPENLGTKVNSDADDMFPFISPDGKTLYFTRKN
                     YFENTGPDLRDDIYVSKLDNDKWTTAINIGPPLNNEQHNFVSWISPDGSSLLLANNYQ
                     KIKQDISISTKTGTGWAFPKTLKINDFYNNNEFSCYHMNTEGNVILMAIERPDTHGDM
                     DIYVSFLQANKVWTEPKNIGGTINTAATEGSIFIAADNRTIYFASNGHSGYGGYDMFM
                     SKRLDDTWTNWSEPLNLGSQINSTGNDYYFTIPASGDYVYFSSTNNSYGKADLYRIPL
                     PKPLQPDPVTLLKGTIIDAETKKPIPADINFGGLVSEEPDGISTSGDGGYQVVVPESN
                     YLISIIKPGYLPYSATIDPELHYDAIDYNPEDSIATIKHEITTTLKAEIKTNPITPEA
                     LEEEVIKLLPDEIADTSSTVRDSIIRDITDAFETKDSTYKEVTADIEMIPIREGTVIR
                     LNNIYFEANKSDLRPESSSALDELVTFLKTNPNIYVEIGGHTNGLPSDGFCQKLSNDR
                     ANKVVEYLIANGIPADHLTWKGYGKTQPIADNATVTGRRQNQRVELKVMKVE"
     gene            complement(16788..17450)
                     /locus_tag="PLM90_05900"
     CDS             complement(16788..17450)
                     /locus_tag="PLM90_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88937.1"
                     /translation="MEIFAYIFYIGVINIVFTSGWKLLATMVGSLLKDVGVNKDNTFL
                     VFKTVGYYILVSLAALVTAQHMETTYKLAAMAYAFTGTFVIYATIASNLERNRWRAVM
                     NFERKRIQVMRYDGYLLMACIVLFIATILQPQIADLAPHRWLLETIDSIYNTIFLKWV
                     IGFFAFFYMLNIIFRGIKFSDEVFAMLFNRKRPGSDNNDNSGTEYADYEEIIDDDSRL
                     PK"
     gene            complement(17571..17831)
                     /locus_tag="PLM90_05905"
     CDS             complement(17571..17831)
                     /locus_tag="PLM90_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ88938.1"
                     /translation="MHAPILFTKKRLLDFSKRERQILQLIAKGMTTREIALELSISEN
                     TVESHRKRMIRRVEARNIFGVFHYTISNGILQPSVFSDPYLL"
     gene            18157..>18534
                     /locus_tag="PLM90_05910"
     CDS             18157..>18534
                     /locus_tag="PLM90_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88939.1"
                     /translation="MHSPAEDLYHLIHALSANEKRYFRLFAQRHGNEKDKLYMKVFDL
                     IMAQEDGYDEEIVKKKLKTAAAIKQLPQTKNYLYDLIMQSMRLYRSDKNVATEVFNLI
                     QDELFYTEKGLTQMRSKALKKAKE"
BASE COUNT         5153 a         3671 c         3948 g         5662 t
ORIGIN      
        1 aaaataaccg tttcaaattt ttgaaataat tgagcaagca aggtcatcac ctcttccgca
       61 taaatgccgg catcataggc atcatgaata ctcaactgtc cgacaagatg atgtggcact
      121 tgagataatt cctcttttgt tggtgcggca gtgccgatgg gaatttcgcg ataaaattgg
      181 cgtgcatctg ctgaaatgat ctctgtttgc aatgcttttg ccagggcgat ggcacatgca
      241 gttttccccg aagctgtggg cccggcaata acgatcagat ggcgattgcg ggttttcatt
      301 gttttatctc ttaacaggtt gcaatcccag tgataatccc ttttccagca tcaaacgata
      361 ggcagcgtca tattcatttg gaatatctcc ttccaatatg gcctcacgta caaattcttt
      421 tatctgacct acttctttgc ccggtttaat atcgaaggtt tccatgatca attcaccggt
      481 tacaggcggt tgaaaattgc gaatatgatc gcgagcttct acatcgcgaa gcttttcttt
      541 caccaactcg aaattctcgt gatacttttt aattttcagc cggttttttg tggtgatgtc
      601 ggcgtgacaa agcaacaaca acccttccag atcgtcgcct gcttcgaata ataatcttct
      661 tattgctgag tcggttacat tttcttttgt cagcgatatc ggtcgcatgt gcaatcccac
      721 aatattctgc acgcgtttta attcctgctg cagcggtagt ttcagatgcg taaatatttg
      781 ttttgccatg cgtgcaccta caacttcatg cccgtggaaa gtccaacctg ttggcggaat
      841 gaattttttt gtcgccggtt tagcaatatc atgcagcaac gcagcccagc gtagccacag
      901 atcatctgta agcgcactga tattatctac tacttccagt gtatgaaaaa aattgtcttt
      961 gtgaccttga ccatcaatca tgtcagtgcc gtgcatggca tataattcag gaaaaataat
     1021 ttccagcagt ccgctgtccg ataaataata taatcctatc gatggcttcg gtgataacaa
     1081 tattttattg agttcgtcgg taatgcgttc ctgcgaaatg atgcgaattc tatccttgga
     1141 tgcttttata cccgcataag tatcatattc aatatcaaaa ttcaactgag atgcaaatcg
     1201 aatagccctg agcatgcgca gtggatcgtc gctgaatgta atggcagggt ctaaaggcgt
     1261 tctgatacaa cgatcctcca tatcatggat gccattaaaa gggtctatta ctgcgccata
     1321 gtcattttca ttcagcgaaa tggccaatgc atttatggta aaatcgcgtc gcaactgatc
     1381 gtcgcgcaat gtgccgtctt caacaatagg tttgcgtgaa tcatggcgat aagattcttt
     1441 gcgtgctcca acaaattcta tctccatgcc atgatatcga aagtgagctg tgccaaaatt
     1501 tttaaattcc gcaacacttg gttttccggg tagggtagcg gcaacagctc gggcaagtgc
     1561 aattccactt cccacacaaa caaaatcgat atcttttttg taatcgcgac ctaatatttt
     1621 atctcggaca aatccaccta ccacataaca aggaaattgc agtgttgccg atacctctgc
     1681 aaccactttg aaaagtgcat gctccgcatc agaaaattgc aattgttgac tttgtatatg
     1741 taggttacct gacatgaatg tatttataca ttaaacagct acgctattat cgcggagtgc
     1801 atcgttcaaa cttgtttttt tatcggtact ttccttgcgc tgtccgatga tgagtgcaca
     1861 aggtacttga tattcgccgg caggaaattt tttagtgtag gatcctggaa ttaccacaga
     1921 tcgttttggc acacgaccac gatattcaac aggttcatga cccgttacgt caatgatttt
     1981 tgttgattgc gttaatacca cattcgctcc caacacggcc tcttcttcca caacaacgcc
     2041 ttccaccact atgcatcgcg atccgatgaa gcaattatct tcaataatta ccggacttgc
     2101 ctgcacgggt tcaagcacgc caccaatgcc aacaccgccg ctgagatgca catttttgcc
     2161 aatttgggca caactgccta cagtagccca tgtatcaacc atggtgcctt catcaacata
     2221 cgcaccgata tttacgtatc cgggcatcac gattacattg cgtgctacaa aggcaccata
     2281 gcgaatgaga ccatgtggca caacgcggat tcctgcttct ttgaggttat gtttcagcgg
     2341 aattttatca tgaaattcaa aaggtcctac gcttatggtt tccatttgca tgataggaaa
     2401 atacatgatc accgcctttt ttacccattc atgcacctgc cagttgccat tcaccttttc
     2461 cgcaacgcgt aaactgccgt tgttcaacat ggcaattact tccttaatgg tatgctgcat
     2521 ggctgtatcc tgcaaaaggc tgcggtcgtt ccatgctttt tctattaatg cttgcatgct
     2581 gcaaatttag ggatattgca tttagtagtc ccgatccggg caatatctct gtaattgtcg
     2641 ccttaaaagg tatgtgcgga tgtgcttata tttgacgttg caacaattca gctttccacc
     2701 cgatgcgcgc aaaattaatc atgttattag gacctgtcct tgcagtaatc accggcctgc
     2761 tcttttatta tacgggcaat tcgcctgagg ttagtaaaat ggctgctgtt ctggttttga
     2821 tggcctattg gtggatgacg gaagccgtca atatatttat taccagttta ttgcccgtag
     2881 tgttattgcc attgttaggc atcctgagta tcgacgcggt tgcaccgcaa tatatgaagg
     2941 atgtgatatt tctctatatc ggaggattta ttctcacttt cgcgattgag cgctggaatt
     3001 tacataggcg attttcatta ggtattttgc ttcgctttgg cagttcgccg gaaaaattgc
     3061 tcattggttt tatggttagc acctatttta tttcaatgtg gctgaacaat acctctacca
     3121 cattgatgat gctgccggtt gcactagccg tgattaagca aattcatggc aacgatgatt
     3181 ccaaacgcac aggattatct accgcaatat tattggggat ttcttacgca tccaccattg
     3241 gcggtacagc aacattggta ggcaccttgc caaatatggt tatgaaatca ttttatgatg
     3301 aacatttccc tgccgaacaa tccttgaatt tcgcaaactg gttctctttc gcattcccaa
     3361 tgagtttgtt ttttctggcg atatgctatt ttgtttttaa ggcaatgctc cttcgtaatt
     3421 ccgatcacaa agctccggat ctggaatatt gcaaaacaga atatcgcgca cttggaaaaa
     3481 tgactttcga agaaaaagcg attgcatgga tttttttggc tgctgtagta ttgtggttta
     3541 cactcgacaa taaaacctta tggggtctcg atctggtagg atggaaaact tatctagtac
     3601 attggcatat cattccaaat gataagttcg tgaatgaaag ctttgtcgca atattaatgg
     3661 gcatgatatt atttttcttc ccgtcaaaat ctaaaaaagg cgagtcgttg gtaatgtggg
     3721 aagatgtgaa gcggttgcca cttggtatat tatttttatt cggcggagga ttcgcacttg
     3781 caaaaggtgt tgaagtgtcg ggactggacg attggattgc aggtaattgc accgggttaa
     3841 aagaccttcc acccatttta atgatcttat gcctttgtgt tatcactacc atattttctg
     3901 aatttgcttc gaatacggca accgtgagtt tgtttatggc cattttaatt cccattatcc
     3961 cgatgcttgg tataccgcca ttgctggcga tgatgccgat caccattgct gccagttatt
     4021 cattcatgct gcctgttgga accccaccta atacgatagt tttcggaacg gaaatgatta
     4081 aagtgcgcac catgctccgg gttggtttat ggctcgattt acttggtgca ttggtcatcc
     4141 tgttgttcac ctttaccatt gggcaatggg tgtttggatt ctgaaagtca ttgctatacg
     4201 aaatcaatta atgccaatgt tgacaggcaa aaaaaaagac catcaacaga tgatcttttt
     4261 ttgggtttac gctacaattg tttggtcaat gcgttacggt aagtatactt ccttgcgtat
     4321 ttccgattgc ctgaacacga ataatataat ttccttcagc caacattgag gtattgatag
     4381 tataatgctg ctcaccggct gcacaattag atgtattcac ggtcataata tgttgcccta
     4441 acatatcaac taactctata aataaatcgc ctccggtgtg ttgaacggta attgaagtag
     4501 attgattcgc aggattcggc gatatcatca aattttggtt aacggttgca gcagttattt
     4561 ccgtcagtcc ggtcacttta agcgcttcag attttttata tgcaatatct tcatcgccat
     4621 cggctattac ttcaccattg tcatatattt tgtaataacc ggttcctgtg ataccatcac
     4681 cacctgaatc gaacatatgg aaagtatagc aaccgttgcc aggcaaggtg acatcttcgt
     4741 ctacaaccac tgtgttttta tcgctggtag gataaggtcc gcctgaatgc acttcatcgc
     4801 cggattcttc atcaataatt tcccagcgtg tttgtgtcgg ataattatct gttttcactt
     4861 caagatgtat caccatattc acattattat ggaaaaagat agtggtgtat acggtattat
     4921 ttaagtcatt gtcatcggca gaagttatgt tgatgtctat gtttgatgat tcatcatcca
     4981 atacaacaga gccaatggtc acttcctcca tttcataaat atctaaattg cctgtccagt
     5041 tataactcag caattcaacg ccgtcttgat tggcagtaat tgtgcatgca gtaagtgggt
     5101 caaatcccat attttgtaaa gtaatactca attcaacttc cgcacaacct accgtaggac
     5161 cggtataatc cataatgctt gcatcgttgt tggtgaatgc aacggcacaa tcatcagtgc
     5221 ttgcatatag aacagccgtt gatacctggc ctacctcatc aactatgcga ttaggacaaa
     5281 tgcgatatat tgttggaaaa taggctattt cataatcgct taccacggta taacttggat
     5341 caccataatg cgttaaaata atagggtagg gcgtgccatc aacccaatcg ccttgtgttc
     5401 cggcgccgat gccattcaac tcatcgagag ttgaggctcc atcaccttcg atatagaaaa
     5461 ccattacctc attggtgcca tcaggtccgt atgtgtcgta taaggcttcc aggttacccg
     5521 agttgtggta tgtccagcaa ggtccgcacc aaactgcaga gaaatcgagg aaaacgcttt
     5581 tgccttggtt caggatatcg tataaatgcc attgcgtgcc attgatatcg gtgagcgtcc
     5641 agtcttcagc tatagatccg tcttccagct gtgctttggc cggagcaacc atcatcaggc
     5701 tgatgataag caatatacag gggtaggtaa tttttttcat gatcagggtt ttggatatca
     5761 aacttaggaa atttatcgga aacggatgat ggtgagtggt gagtggtgag tggtgagtgg
     5821 gtgagtcacg aatcacgatt cacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnntggtga gtggtgagtg gtgagtggtg agtgggtgag tcacgaatca cgattcacga
     6001 ttcacgattc acgaaagaca gcttcgttta aataaaaaag ctcccgtacg gaaggtacgg
     6061 gagctcaata tctagggaat tcctaattaa tggaacacag taaatttcac agtattgttt
     6121 acgttttcac cagtgatttg aacgaaatac attccgtttg caagatcagc agtgttaaca
     6181 gtaaagctat gagcacctgc agtaagtgtg cctgaagtag cagttcctac aacagcacca
     6241 tacatgttaa caatagtaac atctacatca gcagtagctg tgaggttgat gtctacattg
     6301 atattgtcgt tagcaggatt aggataaagc gaaacattgt ttatagcgct gttttcttca
     6361 atagcaactg cagtagttac tttcaaagca gcagattttt caaagccaat gttttccatg
     6421 ccttgctgga taaggtcacc gttattatca tacaatttgt agtatcctga acctgtgata
     6481 ccatcaccat aagcatcata gcatgtaaat gtaaagcagc caagagaagg taatacgatg
     6541 tcttcatcga acacgatctc atttttttgt gcattggtat aaggaccacc ttcagcaact
     6601 acatcacctg tagattcgtc gataacttcc cattttgttt cagtagggta gctatcggtt
     6661 ttcaattcga ggtgaatgat ctgagtgata ttgtcttcgt aagtaacggt tgtagcgata
     6721 acgttgttta cggtattttc atcagcagat gtaatttcaa ctgaaaggtc aacatcagca
     6781 tcagctggtg tgaaagtacc aacatcaaca gttgcaattt cataagtgtt caatgaacct
     6841 gtccaatcaa cagaagctac ttcaatgcca tcagcataag cagtaatggt gcaagcagta
     6901 agttcgttga agcccatgtt ctggatagta acagaaacag gaacatcgcc gcaagaagcc
     6961 acagggccat tgtaggtcag gatgcttggg tcaacgttaa ctgaagcagc agcacaatca
     7021 gaaatggtag catacaatgc tgcagttgaa agctgtccaa cttcatcaac aatacggttt
     7081 tggcaaacgc ggtagattgt tgggaagtaa gcgatatcat aatcctgaac agctgaatag
     7141 ctaggatctc cggcatgtgt cagaacgatt ggataaggag tacctgtaac ccagtcgccc
     7201 tgagttccgg caccgatacc attcaattca tccaatgtag aagcgccatc accttcgatg
     7261 taaaatacca ttacctcgtt agtgccatca ggaccgtagg catcgtataa gccttcaaga
     7321 cttccaccgg tgtggtatgc ccaacatggt ccgcaccaaa cagccgagaa atcaaggaat
     7381 acatcgtaac cctcatccag gagagtatag aggttccagg tggtaccgtt tatatcggta
     7441 agggtccagt caggggctac agagccgtct tccaactggg cttttgctgc attgttgaat
     7501 ccgaagattg ccagggcagc aagtgaaaga gagaggaaaa gttttttcat ttttcgctga
     7561 ttagaatatt acttaccaaa gataccagaa gttgcgtaaa atcccaaata actgtgtgga
     7621 catctatttt atgataatcc cgtgaccgta aaccttcagt aatccactct aagactataa
     7681 atgcctgtat aaatgacaat gaatcagagt tatattttgg agggaatcta tacctgatct
     7741 ttaaataaaa cttataaaaa tgtaaatatg ttatgatgaa ttatctaaat tattatatta
     7801 tataatcatt gtaattataa gaaaatcaat aaaataagat taaaaattga tcaaaacgta
     7861 tatgtcgatg ttatttcacc tagtccttcg atagaaacta caataggata ggtttaattc
     7921 tattgaaagc agtggttagg ggttgcataa ggttatcaga ttcaatgttc catgaccaaa
     7981 ccataaatct caagatcata ctttttccct aaaacatggt aacaagattt aagacttgtg
     8041 cacaatatgc taatgcataa tagtgtttga gccttatttc tgatggaatt tcggcttaaa
     8101 atcggctggg aatgagtaaa aataaaccgc tccggagtgt agcggtttac atataagttc
     8161 aaatcatcaa tttccgcccg acttaccagt atttggatcg gtggtagcag aacattgtga
     8221 tagcatttga ttaacctgat ctaatcccca atttggatga agatcgcttt ggggaacaaa
     8281 tatgctgtat ttctgtaacg ctgtttcaaa cattttacag ccgggttcct taccgccgcc
     8341 aaattgtggt ggggtgtaaa acaggctttg tcccatgatg taatatgatc ttggattatt
     8401 tggatcggca gtcatggctt gttgcaataa catcatgctc tgcatgccat acatttgtcc
     8461 gcgcgacatc ggatcaacca ttattctacc accaaaaatc atgctgcgaa ggacaaatat
     8521 ttctgagttg tttggtgata aagaatctgc tttcatggca tgtttttcag caacatccag
     8581 cacagcatcg ttacgcgtta catcgttata aatgaataat gcagttgttc gtgccaaggc
     8641 tgaatagtaa tagggcagcc attgattttt ttctgccatg gcaatgcgtt caaagctatt
     8701 ggcaacgttc ataaaatcat ctccggtttt ggcgctatcc ataagcgtca gcgctttttt
     8761 cataccgtcg tgaaacttag cagtttgtgc ttgagcgcct attgaagtta gggcgacaag
     8821 tgttacaagt aatatttttt tcatagtaag gtgcttattt acaaaaagtg ttccgaattt
     8881 gaatgttatc atttgttcga cttatcgatg tcgatatcgt ctacgttttt atcttttccg
     8941 tataaaatgc tcataaagat gccgaaaaag actgtactct tcacaggagc ctgatttaca
     9001 aggaattgtg agccatcggc ggagtagtta tatccataga tctgatcaaa tccaaaggtg
     9061 ttatcccacg aaataaagaa gacagtgaaa ttaccccaaa ggctctgcaa tacactggcg
     9121 ctaagtgcaa cattatgcag atccggagat acatcgccca aaaattcggg attgtttgga
     9181 ttgtaatatg gccatccgga aatatagcga taggtgccgc tcacttgtgt attgatgccc
     9241 ggaaaataat gtttgtacac caagctaaga atatgttgcg cagcgaaagt tggtgttact
     9301 tcttccggat aatccaagaa tttgcgctta gtgtcaagga agctgtagga aatccaaaag
     9361 tcggcataat caatagtttt tttatctctg tagaataaat caatgccacg agcatatccc
     9421 gatccgttgt ttgcggtaat cggtacgaaa ggatttagag aatccgttgt cagtgtaacc
     9481 aactgatcat aggttttata atatccttct attctgaacg tgcgatcatt ggtgatgtat
     9541 tgataattgg cgatataatg tgttgctttt tcatattgca atggatagag cgcctgcgta
     9601 tacaggtaat tgggttgcgg cgtttgatag aaatcgccgt aggcaagtga aacctgcgca
     9661 ttttttcctg ttttgtatgc taatgatata cgcggagcaa catttacatc atccagtatt
     9721 tccgaatatt cagctcgaac accaatgcgt cctgcaaggt cgttggtgat aaaaatgtcg
     9781 ctttcggtga atgcggccgt gaaattgtcg gtagcatcaa attccagatc accggcaaat
     9841 tgatcgtagg tgatatgttg aaattcgccg ccgtagcgca atttaatttt ttcggtaatc
     9901 tcattattga aggttacttt tccctgcgca ttttgatcgg agcgtccaac attgaaacca
     9961 tctacaagga tatcatcatt gtttttactg tagctcacac ctgcctgcac accccatttt
    10021 tttgtgagga tttctcgcca gctggcgttg atataattgt aattatttcc taaataaaca
    10081 tcatcggtag ccggcagact gtcgatatcg ggcgcacgca atgccatgtt tgaagattcc
    10141 ttttgggcat atagtttgag tatacctgtt ttagatgttt tctgacgaaa gaataagctg
    10201 ccatcaacag attcaacact atttatccaa tcgaaagtag ttggtttgaa cacattatca
    10261 aataatccga ggttggtgta attaataccg ccacctaatg ctgttttttc atttttaaat
    10321 ttctgtgtgt gaaatccgcc taaaaatatt ggtgcgacac ttaatcctga tgcactgtat
    10381 tccggcatgt cttctgtatt taaaattaca gcagatgaaa gtgcttgtcc gtattccgct
    10441 gaatatcccc ctgtgctgaa aacggtgccc ttgaacatga agggttgaaa tcgacctctg
    10501 ctgggaacat ccggcacagt gctgtaatat ggattattca ccaccatgcc atcaatgatc
    10561 gtttttgttt cgtaatcgct accaccgcgt acgtacagac catcgtcgtt gcccacctta
    10621 ctggctccgg gtaatgtttc cagggcaccg taaatatcgc cttcggcacc ggcggtggtt
    10681 acaatatcga gcggtttgag aatggtattc tttttttcat cgcttgcttc aaatgcacct
    10741 gcagtgatca caacggcatt caattcatta aatgcttcct tgagcacaat attcaccgat
    10801 aaggtgcctc cgtttaattg gagtgtgtcc tcaaaacttt catagcctac aaagcttacc
    10861 gagagtattt gtttgcctgt ttctgtggtg atgaagctat atgtgccatc cacattgctt
    10921 gttgttccgt cgtaggtgtc tttcagaaag atgttggctc ccggcaacag attctttttg
    10981 ttatcggtaa cagttcctga aatggtggtc tgtgccgtta atgccgttat tgaagaaata
    11041 ccaataaata ggagtatagc ttgtttcatt gatctgattt cggagaggta tactaagtta
    11101 ttttatgcaa agtaacggga taacatgccc tttggcaaaa ttgtttggac gatcagggtt
    11161 caggtatcga ctagtggcga caaaagagcg acgaatagtg acggcttttt tgttaatctt
    11221 gcaaaaaagt aaagctatgg caatagtgat gataacaggt gcaagctccg gttttggggc
    11281 tgctacagca gaaatttttg ctcaaaacgg atatcgcctg attttaaatg cgcgacggat
    11341 agaaagattg gaaacactca aacgcatgct ttcggagact tatagcgccg atgtattgtt
    11401 gttacctttc gatgtacgtg ataaggatgc cgtagcaact gccatggata ccattcctgc
    11461 ctcatggaag caaattgatg ttttgattaa taacgccggt cttgctgcgg gaagagattt
    11521 atttcaggat ggtttagttg acgattggga ggcaatgata gataccaatg tgaagggatt
    11581 attatatgtc accaaacacg cagtaagcgg catgaaagag cgaaaaagcg ggcatattat
    11641 caatgtgtcc agcctcgcag gacaggaggt gtatcctatg gggaatgtct attgtgctac
    11701 taaacatgca gttacggcat tgagcaaggg cttgcgcatg gacttattgc catatcatat
    11761 caaggtaacg aatatctctc cgggactggc agaaacagaa ttcagcatgg tgcgatttaa
    11821 aggtgatgag gcgaaggcta aagccgttta tgaaggattt gatcctttac aggcaaaaga
    11881 tattgctgat gtaatctatt ttgcggcatc aagacctcag cacatgaatc ttgaggagat
    11941 tactatactt ccaactgcac aaagcgacag tagaacggta gtgcgtgggt gatggtaggg
    12001 ggtgaaggtt cgaagattcg aagatttgaa ggtttgaagg ttgaagattc gaagatttga
    12061 agaatcgaag atttgaaggt ttgaaggttg aagattcgaa gatttgaagg tttgaaggag
    12121 gaaagaatcg aagattcgaa gatttgaagg atagggatta ctagatacta gatacttggt
    12181 tctagatact agatactaga tactagatac ttggttctat atactcgatt ctagatactc
    12241 gattccacca agaggttgag ggttgtagat tgaaagttaa atgttaaggt ttatagaata
    12301 acgaaccaat agatctttgg tatgcggatc agtgaattcg gattcaaagg acatgccgag
    12361 ttttgtcaat aattttatgg aatgcgtatt gtcgggggtg gcgatggcaa gcatggtatc
    12421 aatatcggtg taacctattg caaattccat taatgcctga gctgcttcaa aggcaaaacc
    12481 ttttccctga aattcaggta ggaatgcaaa tccgagatcc ggatgcggca gggtgtcgcg
    12541 gaccacaatt ccacacaatc ccatagcagc gcctgttgtc gtatcgcgca ctaaccacat
    12601 gccataatca tccttttcat acgctttcat gatgcggttt tgcatatagg aacgcgcttc
    12661 ttcgagggta tgcacatttc tatcgccaat atatttcaac catcccggtg aatttaataa
    12721 tgctaataaa aatgctgcat catcaatggt gcagggtgat aatgttaatc gttcggtatg
    12781 cagagtttcc atgtttttta tttattcttt aacaaccatt tcgcatagtg tgcacgctgt
    12841 gaatcatttg catcatccat tgctttcaag cgataattca ccttgtctgt tatttgtatg
    12901 gtcgccgaaa ctgtgtgcat gcgtccggac ttagacaata ttatctccag cttatcaccg
    12961 gtttttgcat ctgcagttaa actagccaat aatccattgc tatagcgata accatttatg
    13021 gcaatgattt catctgtcac ataaattccg gcttgttcac ctgctgaatt gcgacgcacg
    13081 gcggtgatga tgttattgtt atttatggta atgccaagat ctgattcaat agtattttcc
    13141 agtgtgtttt ctaattgaag tcctacatga cataatatat cgttgaatgc aatttgtgaa
    13201 gtgccgaata catattgacg ataaaagtca ttcattgata taccggcgaa ttcttctatc
    13261 acagctttga attcatcttc tgtatatccg gaatcgcgat ttacataaaa acgctcatag
    13321 gcagtgcgca tcacattgtc gagattgtgc ttgccgtctg agtggtggat gatgagtata
    13381 tccagtaatg ttgccaatac acctcctcgc acataataat ttacctgact gttttcacta
    13441 ttttcatttt ggcgataata ttttatccat gcgtcaaaac ttgcttctgc aaccgacatg
    13501 atttttttac cgggggcatt ttctgccaga ttgatatttt tttcaactat tgtaagatat
    13561 tcctcttctg catacaaccc ggttcgatat actattaaat catcataata gcttgtgaac
    13621 ccttcggcaa tccaaagcga agtggtgtag ttttcggtaa tataattaaa gggccctaat
    13681 gcttttggac gaaggcgttt tacattccag aggtggaaat attcatgtgc aaataatgaa
    13741 atgaattcct gataattact tcgatcggca aacatcaatc gcttaaaaat taaactggtg
    13801 ctgtgtaaat gttcaagtcc tccacgaagg gtgttgctgg tattgaggat gatgagatac
    13861 gatttgcaag gatgatgttt aaatatattt acttcggttt caataacagc ctgcatatcg
    13921 gttatcatgg aaggaatatg ataattcccg gtgccggaaa tcaccaatgt atgctcaact
    13981 ccggcagcat taaaatgttg tgttatatgg tttccgattt caatgggact atcatataat
    14041 gtatcagcat cggcagcaaa tctcacccat ggatcattgt ttaccatagg taatgaagta
    14101 gctatatctt taaatgcatg atgtggatga aaacttatgt tgaccggaag cggggtgtct
    14161 tcaatgaata aaaacatacc cgcgggaata atggaagcac gttcactatc taaccaacag
    14221 gtgcgcacac tttcttcgaa ggcgtataat tcataaataa tatctatcgc ctgattattg
    14281 gtagtgatct cccatgtgtt tttcgcagtc ttgttaatgc ctacgttttt atcttcacaa
    14341 tatagtctat tgatgttttt ggaatattcg cgaaccaaat aggagcccgg cgtccaaaca
    14401 ggtaatgcaa gcgaaagtac atcctttccc attgcaggta tatgcatctt aatggtaaac
    14461 gtgtgggtat gcggattgtc catgaataca tggaaactca atgacgacat gtgctaaatt
    14521 tcccacaaat ttggcaaaga aaaccgacac tattccactt tcatcacttt cagctcaaca
    14581 cgttgatttt gacggcgtcc ggtaactgtg gcattgtcgg caattggttg tgttttgcca
    14641 taacccttcc atgttaggtg atcggcagga atgccatttg caatgaggta ttcaaccact
    14701 ttattggcgc ggtcgtttga taatttttga cagaaaccat cacttggtaa tccattggta
    14761 tgtccgccaa tttcgacgta aatgtttgga ttcgttttca ggaaggtaac cagttcatct
    14821 aatgctgaag aagattccgg tctcaaatcg cttttgtttg cttcgaaata aatgttattc
    14881 agtcgaatta ctgttccttc ccggatagga atcatttcaa tgtcagctgt cacttcctta
    14941 taggtgctgt ctttcgtttc aaaagcatct gtaatatcgc gaataataga atcgcggact
    15001 gtagatgaag tatctgcaat ttcatcgggg agcaatttta ttacttcttc ctcaagggct
    15061 tcaggggtta tgggattggt tttaatttct gcttttagtg tggtagtgat ttcgtgttta
    15121 atggtagcga tgctatcttc cggattataa tctatggcat cgtaatgcaa ctcggggtca
    15181 attgttgcag agtaggggag atagcccggt ttgatgatac tgatgagata attgctttca
    15241 ggaactacca cttgatagcc gccatcgcca ctagttgaaa taccatccgg ttcttcactt
    15301 actaatcctc cgaaattaat atccgcagga ataggttttt tggtctcggc atcgatgata
    15361 gttcccttca acaatgtaac agggtcgggc tgcaatggtt ttggaagggg gatgcgatat
    15421 aaatctgctt tcccataact gttgttggtt gacgaaaaat atacataatc gccactggca
    15481 ggaatagtga aataataatc gttgcccgta gagttgattt gtgaccctag gtttaatggt
    15541 tcgctccagt ttgtccatgt atcgtcgagg cgcttgctca taaacatgtc atatcctccg
    15601 tagccggaat gaccattgga agcaaaatag atagttctat tatctgcggc aataaaaata
    15661 cttccctctg tagcggcagt attaatagtt ccaccaatat ttttaggttc ggtccacact
    15721 ttattcgctt gcaaaaagct tacataaata tccatatcgc cgtgtgtatc ggggcgttca
    15781 attgccatga gaatcacatt gccttcggta ttcatgtggt agcatgagaa ctcgttatta
    15841 ttgtaaaaat cgttgatctt taatgtcttt ggaaatgccc atccggtgcc ggttttggta
    15901 gatatggaaa tatcttgttt tatcttttgg tagttattgg caagcaacaa tgagcttcca
    15961 tcaggtgaaa tccacgatac gaaattgtgt tgttcattat ttaatggagg gccgatattt
    16021 atggcggttg tccatttgtc attatctaat ttggatacat agatatcgtc gcgcaggtca
    16081 gggcctgtat tttcaaaata atttttgcgt gtaaaataga gcgttttgcc atcgggagaa
    16141 ataaatggga acatatcatc cgcatcggaa tttaccttag tgccaaggtt ttccggtgga
    16201 ggcagtttct cggcataagc gagttcattt attttggctt tgataggttc gcggctatcg
    16261 gaaataccaa tacaatcaat ttgattcata cctgctatca cttttgtatt gagtgatact
    16321 tttacttctt tcaccaggta atcagttcgt gggataaaga ttctgaaaac gcgacttcct
    16381 gtattgtaag ccggcttgat ggtatcgttg gtgtagacgg tatatttttt cccttcggta
    16441 gaaataagca ctacctcttt aacagcgccg ggggcattgc tttcgcctat accaatttgt
    16501 tgcacatatt gaggctgagc aaagcgcact tgaataaatt ctcctgccgg attatcagga
    16561 gtagatggag cccaggcaac agtagattcg ccggaggcag gaagtttatt tggagcgcca
    16621 agggcttgtt ttgcagaaaa ttgtgttcgg ctgtattcag ttgaaaattt cactacttcc
    16681 gaagcccact gaacgtcctg cgcaaaaagc aattgtgaaa ggcacaatag aaaaatggtc
    16741 aggatttggc gcatataata ttcagataaa cgaattctga agtggcatta ttttggcaaa
    16801 cggctgtcgt cgtcaatgat ctcttcataa tcggcatatt ctgtgccgga attatcatta
    16861 ttatcggatc ccgggcgttt tctgttgaaa agcatggcaa acacttcgtc agagaatttt
    16921 atgcctctga aaatgatatt cagcatgtag aaaaaggcaa agaaacctat cacccatttt
    16981 aagaaaatag tattatatat actatcaata gtttccagta gccagcggtg tggagcaagg
    17041 tcggcgattt gcggttgtaa tatagtcgct ataaataaca cgatacatgc catcaacaaa
    17101 taaccatcat agcgcattac ttgaatacgt ttgcgttcaa aattcatgac ggctcgccat
    17161 ctgttgcgct ccagattact ggcaatggtt gcataaatga caaaagtgcc tgtaaatgca
    17221 tatgccatgg cagcaagttt gtaggttgtt tccatgtgct gagcagttac cagtgctgca
    17281 aggcttacca atatgtaata acctacagtt ttaaatacca ggaaagtatt atccttattg
    17341 acacctacat ctttaagcag ggatcctacc atggtggcca ggagtttcca accgctggta
    17401 aaaacgatat tgatcacacc gatatagaat atgtaggcaa atatttccat cgtcggacaa
    17461 agatacctgt aagtaaataa aaaaaccgcc cccgggggtg gggacggtca gcgattcaac
    17521 agcctatgtt gaagagggag aaacttggga tagcagagtt gtgcttgtgt ttacaataaa
    17581 taagggtcac tgaaaactga gggctgtaaa atgccattgc tgatggtgta atgaaataca
    17641 ccgaatatgt tgcgggcttc cacgcgacgg atcatgcgct ttcggtgact ttcaactgta
    17701 ttttcggaga tgctcaattc cagtgctatc tctctggttg tcattccttt agcaattaat
    17761 tgtaagatct gacgttcgcg tttgctgaag tcgagaagac gttttttggt aaataagatc
    17821 ggcgcgtgca tagtgtttgt tgtttgataa gccaaaggta tttccacccc tgagcctgct
    17881 caagccaaat tttgaccgat tcttcaacgt ctgacagaca aatatgaaaa taattaatat
    17941 gtataacgcc tgtaatgcaa ttggggatag cgttttagcg gttatcagtt ccgttaacgg
    18001 aaaaccgtga attatccgtt tttggatatg aaaaatggta aattaaccga ttattgctat
    18061 catttcaggc ttaataagcg gtttttggca ttgactttga gcagttagtt ttcaaactgg
    18121 agttcttcaa cctatattta cccctgaagc aagcccatgc atagtccggc agaagaccta
    18181 taccacctga ttcatgccct ttcagcaaat gaaaagcgtt atttccgact ttttgcgcaa
    18241 cgtcatggca atgaaaagga caaactgtac atgaaggtat tcgatcttat catggctcaa
    18301 gaggatggat atgatgaaga aatcgtgaaa aagaaattga agactgctgc cgcgataaag
    18361 caacttccac aaacaaaaaa ttacttgtat gacctgatca tgcagagtat gcggttgtat
    18421 cgcagcgata aaaatgtagc cacagaggtg tttaatctca ttcaggatga gcttttttat
    18481 acagagaagg gattgacaca aatgcgatca aaagctttaa aaaaagccaa agaa
//
LOCUS       DAOQOU010000046        18262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1537_length_18262_cov_7.204317, whole genome shotgun sequence.
ACCESSION   DAOQOU010000046 DAOQOU010000000
VERSION     DAOQOU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18262
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_18262_cov_7.204317"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLM90_05915"
     CDS             complement(<1..350)
                     /locus_tag="PLM90_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HMZ88940.1"
                     /translation="MPQTRTVVAFYAVIASMLTFASCSNSRSAMKNVIYTPNAPAPIG
                     PYSQATEAGGFVFISGQIAINPANGEMYTGGDIAVETRQVMENLKAIVEQSGSTMGNV
                     LKCTIFLRDMNDFAA"
     gene            441..3275
                     /locus_tag="PLM90_05920"
     CDS             441..3275
                     /locus_tag="PLM90_05920"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMZ88941.1"
                     /translation="MTRIVIRQLLLVMIFGVTALSVNAQITYTTGWTADSLAAYLAGA
                     GVEIDNATINCHNLAIGKFDCVDCNVGIDSGVVLTTGRVSNILGPNNSGSTGTNNGWP
                     GDPDLNAYPGVGTTHDACALEFDVYAPGDSLKFDYVFGSEEYPEYVGSINDAFVFFIS
                     GPGYPTLTNIALIPGTTLPVTIDNVNATSYPMYYIPNGTGGTGIYATSPYYIEYDGFT
                     TVLTAEAEVTPCEWYHLKLVIGDESDFVWDSGVFLKAKSLVTNVLADFTYPGVPFGGT
                     AEFCTTGTDPAPLYATGATAGTFTCTPSGLVFDAATGAIDVSASDPGSYTLTNTLLTG
                     ICYGDTATWDITVNIYEPDAAGFSYPGSPFCANESDPSPIPMIGASLGTFTATPAGLV
                     INAATGTIDLSASTPGTYTITNTVASGTVCPDGVATNTVTIYPTYAVSQSGNICLGDP
                     FILPDGATVYTGGTYVTTLPTIHGCDSVITTTLAAHPVYNYTLNPAICSGATYTLPDG
                     TVVSTTGTYINNFTSVWGCDSNYTINLTVSPVPVVNMNVHICSGETYILPDGTAVGVS
                     GVYPVTLTTVAGCDSTINTTLFVHPVYAITQNPVICDDVTFTLPDGSVVNTTGTYVTN
                     LLTTKGCDSIITTNLTVNPTYDIIMNPEICIGDTYTMPDGSVTGTGGTTVFPYASING
                     CDSIITVNLTVNPLPVITWPIDDIFCLEQNSIALEATPPGGIYSGPGTSGDHFLTNDA
                     GVGGPYTLTYEYTDANGCYSSMTVTTSVDDNFVTAWGDTTLYYGSSAILHSESGGDYT
                     WSPTNGVECPLCPESYVLPPYSIDYIMTSVDENGCVASDNVMITVLPLPPNDFFVPNT
                     FTPNGDNINDIFYIFGYNVLSITDFEIFDRWGKVVYRGENLDANDINLGWDGTINGDP
                     ANNGVYAYYITVQFDNGQQMKRAGNITLIR"
     gene            complement(3293..4771)
                     /locus_tag="PLM90_05925"
     CDS             complement(3293..4771)
                     /locus_tag="PLM90_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ88942.1"
                     /translation="MKPVVICNTYFLHLDPKQERRMEPYPPLGTLYAAAVLREKGYDI
                     HFFDTIFCSGPEAIFNTLSKFQAGIFIICDDGFNYLTKMCLVNMREACSAMIKKAKEE
                     GFTILVSSSDATDHWQDYLNVGADAIVIGEPEQTIAACVDALQHENPALNTIEGLGLR
                     TTTGFAKTPARALMQDLDNLPLPAWDLIDMDQYRSRWMAHSGYFSINMTTTRGCPFKC
                     NWCAKPIYGNRYHSRSAKAVADEINLLRNTYQVEHIWFCDDIFGLKPNWINSFAGLMQ
                     EQPIPFKIQCRADLLLDENNIQSLAGAGCDEVWIGAESGSQKILDAMDKGTTIAQITE
                     SSRLMYRSGIKPCFFLQFGYPGETLTDIKSTMQLLRNCKPYDIGISISYPLPGTVFYD
                     RVFAQLSNKQNWNDSDELLMMYHGTYSAAFYKYLHRYVHRRFRMLQGMKCIREIFSGK
                     RNTTSPPLKRLFALPIHSVLCLYYFLMVQTKKQPVSELQVAS"
     gene            complement(4768..5727)
                     /locus_tag="PLM90_05930"
     CDS             complement(4768..5727)
                     /locus_tag="PLM90_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88943.1"
                     /translation="MNSNLHIVKEEQFENASTLERAILRALIYFDIFQYPLSKQEIIL
                     FCNIVPENLKSVERVLDRLCNRLLIFKFDDLYSIHNSIGLADRRRCGNKGAIEAMPKA
                     IKRAKLIQAFPFVRSVNISGSMSKNYFDADTDVDFFVITSKNRIWLTRLMLTFFKKVF
                     LLNQRKYFCVNYFIDTEQFLIPDKNLFAATEIVTLKNVTGAKYYHQFLQENAWVSDYF
                     PNFEMPILITQNSRMPVFKRSMEKLLSGKFGDRMDDICFSLMQFFLRRKYRYLNETQF
                     EVNMRGNKHASKHHPQGFQFKVLEAFNKGCHQFSQKHDFPLDA"
     gene            complement(5724..6374)
                     /locus_tag="PLM90_05935"
     CDS             complement(5724..6374)
                     /locus_tag="PLM90_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ88944.1"
                     /translation="MKFFTAWFVQPYVRRKMQRSSFYKWEDVTLFLPPGVFHPGYFYS
                     TKYLLQFILKQHISGKQLLELGAGNGLISIASAKRGAKVSASDISKEALRSLRDNART
                     NHVGLTIIESDLFEQIPDAPYDYIVINPPFYPLDPRNDLERAWYCGQDYAYFRKMFEQ
                     LPVFMHPQTKVYMILSEDTGIGQIENLAGFGNLRLEMTEKKQIFYEWNYIYEIVRA"
     gene            complement(6386..7903)
                     /locus_tag="PLM90_05940"
     CDS             complement(6386..7903)
                     /locus_tag="PLM90_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ88945.1"
                     /translation="MTKEEAQFGILLFNPRAAKHKARIPNSILSIAAAIAGKYPYIIV
                     DGNREDDPLSIIMQHLQTGKFNVFACTVMPGPQLKQAIPITKQIKSLFPDIYIVWGGY
                     FASNQSGVVMQSGWVDSIVYGPGDRAFPMLIDALEQDGPLDMIPNLIWKNGGEVHKNH
                     KDELYDQDTLPALPYQALHDFYDMRGYLGKTILGNKTIAYHSSIGCPFTCAFCGIVPI
                     YNARWKGRSAELIYKDIQFLKNTYGGDAIEFHDNNFFVSEKRTADFSTLMLNENMQWW
                     GEGRIDTIHKYSDDTLQLMRDAGCKMIFFGAETGNDAVLQLMDKGGTQTADQIRAFAA
                     RLKKFDIIPEYSFVLGTPGKDEAETLQRIEEDIAFIREIKSINPQTEIIIYVYSPVPT
                     EGSELFSAVQAGGFHFPQQLEDWISPAWESFDLRKNPLTPWLKPYMIDKIRSFEVVLN
                     GAYPTVSDIKLKSFDKKLMRSLASWRYKTEFYKFPIEVKLMQKLLRYRQPEIEGF"
     gene            complement(7900..8937)
                     /locus_tag="PLM90_05945"
     CDS             complement(7900..8937)
                     /locus_tag="PLM90_05945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ88946.1"
                     /translation="MTKLILVSPGFPADENDSAAVPYLQDYCLHLLKKVPAENINIVS
                     TQYPYTPKPYLWNGIQVYPIGGKNRKGIYQLINHIKTKKRLKSIVGKEPAVIHAFWLS
                     DAAMSASSFAAKRKLPCLVTLMGQDAKAGNPYFKLVSKHVAFTSLCNFQANVFRNDYQ
                     TVVKAVIPLPLPSIDLRPVISRSIDLLFVGSYIQVKQPLQFIEIVAEIKKKHSGVRAV
                     MIGGGPLLAEINSRVVALHLQDHIVVLESINRDEVFEYMQRSKILVHTSSFEGQCNVY
                     GEALLCGMHVLSYNVGYIADSPKHIVCADKNEFVLKIMQLLLHPINFTPVRSIDDEQC
                     INAYLNLYGMS"
     gene            complement(8934..10304)
                     /locus_tag="PLM90_05950"
     CDS             complement(8934..10304)
                     /locus_tag="PLM90_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ88947.1"
                     /translation="MQVILTHGYSIIDDVREQQIMKPYAPLGILYISGWLDAHGINNH
                     VIDTTFITQDEQWNQIKEAKPEFLGVYVNLMTRKNVVQLIKKIKQEINLKHTKIILGG
                     PEVRNNAQQWLQTGADVLVVGEGEITFETLVTSWKTHKDLYQIAGIYFLDESGNMHFT
                     GEREKIKDIDILPFPARQHISIQKYLEVWKSRHGKNAISVSTMRGCPYTCKWCSRAVY
                     GMSYRRRSPQKVVEELQHIQEVYNPDTIWFVDDVFTISHKWLEEFARCVKEANLTLPY
                     ECISRADRMNAEVIHLLKESGCFRVWIGAESGSQRIIDAMDRRVKVESVQEMIRLSNA
                     NGIETGTFIMLGYPGETELDIVQTVKHLKAALPDLFTITTAYPIRGTELFAEIQNDII
                     NEPDWANSSDRDVTFKRNYPESYYKHAVRYVVNETNYAKSKAPLLKLKSMLARMMMWY
                     TRKFAV"
     gene            10437..11789
                     /locus_tag="PLM90_05955"
     CDS             10437..11789
                     /locus_tag="PLM90_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ88948.1"
                     /translation="MAIKIVNDSNLKMHTRYTTEQGTGMVPLLKPINFLGPKPTGTKI
                     DVPVEVADLAVHNATKIFNVTGTGLDGEGSVLAVLDTGIYAEHPDFGARVILSMNYKG
                     EKLPTSDIHDLHGHGTHVAGLAAAGRSKALAKFSGIAPKAKIVSVKVTEGNEDTTTWK
                     RIADGLDQVLNYNADAGTKPYNKVSCVLICFNSFDNFNNANEFDTTNNRLMKLFKICC
                     ESNIVVVVSSGNHFIDFADVSSRTITDGFGKKHKTPQVLEHTGLAFPAVSQHIICAGA
                     TANDDGIYYVSRSDLGDTVSFQKDDMAIFTQRIRKETNDTSNLSLFVAVPGLQMVSLD
                     NRPIQHAVLPGQYPQGYTVMSGTSQAAGVLAGMVLLLQQKIRNKLHIHESCVPIQKIF
                     TALKSTGEERNFDGFGHTVQPPTSTTHAAIGGLTGAHSPFRNFRIFDLIKAINYIQNH
                     "
     gene            11793..12485
                     /locus_tag="PLM90_05960"
     CDS             11793..12485
                     /locus_tag="PLM90_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88949.1"
                     /translation="MALTFPTNMLANKPYVYLDESGTPLKIEVYVKGPQTGAAYALQA
                     SGAPQNSNTYLLTLTLTTVTTGSQVVKTIIDCSTLNGFSSTNFSKIVVRVVYQNNMVG
                     RTTIKIGANDFDAVDYKLFSYLQFIRTGTLKYHGLLELGPDELSQVNISQPSNPVGNQ
                     SDFIMRLNVNVVQANIYTNAVTPHFTYEIGSADGYDPTTYETLELRVDGSNPKKTKGS
                     TTDSEADASGDD"
     gene            12534..14411
                     /locus_tag="PLM90_05965"
     CDS             12534..14411
                     /locus_tag="PLM90_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ88950.1"
                     /translation="MRSGAFYLFLLLVSYSFLMNAGTGNDTARINALNKMSFDLIKKD
                     TTQALELAKSALNLSEQINYLHGLGDAYTRLGLLDKNAGRLNEAVSNYKKSLHYRSAD
                     HDSIGIAKCYTNLGNVYNEKEMIDSAIFYQLSALRINEKVAEKGVVVDNYNNLGLSYK
                     NNGDYALAEEYLLSGYQVAKKTNNIEEQRLARICNNLANVYYEQGANMAKAKPYLLET
                     ISLADSVSDDLLADASNTLGLILMEQNPLPKDSILFYFQFAYNIYKSTGSTELAAVTS
                     NLGDFYVQTGNDELAIRYLLESNKICTDQELKTQLITNYKLLATVYINQGRTADAYEA
                     VSQALALNEEVYTLEKTEKIAQLQLAYETEKKDRIIAEEKATSAMQAGVIARQRFVGS
                     LLAGGTLIIIGAILFYMRQRQLKRKLEYEKKLADDRLRISADLHDEIGSALSSIGLYS
                     EVLKNTLQASDYPAAINYGSEIANTSRELMDNMSDLVWAVNPRNDSFEQLTTRLSNFG
                     YKIFGAGNTNFKVEITGDISSKEVPMAVRHQIYMILKEALNNSAKYAQAKNVLLSIKY
                     KPDSINLSVTDDGRGFDIANAGNGNGLQNMRRRAENIDAQFDICSGNTGTQLTFNIPI
                     P"
     gene            14476..15123
                     /locus_tag="PLM90_05970"
     CDS             14476..15123
                     /locus_tag="PLM90_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ88951.1"
                     /translation="MSIKVCVFDDHRRVRDAMSALLKGTPGFEWGGGFPNAENVVKDV
                     EKADPDVVLMDIEMPGLSGIDAVKMLHAKFPDVRIIMQTSFDEDRKIFYSICYGANGY
                     LLKNTPPHQVLEAIKEVYEGGAPMTPSIARRVVELFRSAAMEKPMAEKDYQLSDREFE
                     VLQLLAKGKSYKLIGEALFISAETVHSHVKKIYDKLQVASKTEAVAKALQENLIS"
     gene            15291..15812
                     /locus_tag="PLM90_05975"
     CDS             15291..15812
                     /locus_tag="PLM90_05975"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88952.1"
                     /translation="MKGIFYVIVCYCYLITNVDGQTISSFVIATDGQTFAAHGNISYT
                     IGETCIRTFDKYGYTLEEGFQRVTTIPEPALDTTIFEADNVTGLSPEIVISCYPNPAT
                     ALITIECDAMLISGAEGTIYNLMGAEVMQFKFEHEYSTQLDVTSLSAGQYLVAIQSPK
                     GFVTAKQIFTILK"
     gene            15819..16658
                     /locus_tag="PLM90_05980"
     CDS             15819..16658
                     /locus_tag="PLM90_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88953.1"
                     /translation="MKTSKLFLAIFLLIPLWGSSQDALNYQAVIRNPSNELVINTEIK
                     FEFGILVGGSQVYAERHAVYTDMNGQLSLKIGNGLVISGVFDSIKWGSDYMEILIRCD
                     IDGDDIYDIITTEALNSVPYAHYAQTAVSAQTSKDNKWAGSGEMIYTISPETNVGIGT
                     TAPMTKLSVNGAISLYPEPDVIVITYDNYFLEVGDRSYLQLQSDDTLAANRTIILGDG
                     ITPGQIIILQGFMMSDTFLELSDGDASNINIPQTFIIGNDDVVTLIWSGISWVGVSIS
                     DNE"
     gene            16934..17908
                     /locus_tag="PLM90_05985"
     CDS             16934..17908
                     /locus_tag="PLM90_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88954.1"
                     /translation="MSRLNISGSVSYLDTTPAKNATVRIIDQDDRSSDNVILTATTDS
                     RGKFVGRTIDWKDEPDGFWPWEHDFMNLTFEVTDSIGRRHTGPFIYVNAAISVPILLP
                     FTKPAIRSENRALLVLADLSQVLTGTLKNFRPLYQFSESNGVNLVNANLRGYYHEIKV
                     LGPGDVTLDRIVSNLSRMTGNSNIWEVDLIVFLHGGRDSLTLSNDTVHMTTIVNAIRD
                     ANLPASKLRMVYSTACYGASQADNWVSAGFNCASGSKACNANGAVELLPFLLKWREGK
                     TFGTCISESENLVLSAPFDAAARLMLPDQVVNSDKDVIGDGTITISSF"
     gene            complement(17933..>18262)
                     /locus_tag="PLM90_05990"
     CDS             complement(17933..>18262)
                     /locus_tag="PLM90_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88955.1"
                     /translation="FSLKGNFKQAKRRKSKNAVIAKTGGENSLPVWYYSIEEIVEFAG
                     DNFNLVFSRPIGLCIPPVYGKKAFWHNTMLIALLRNIETAIGGLKLFRNSGDHLFITL
                     QKNEADQ"
BASE COUNT         5313 a         3709 c         3685 g         5555 t
ORIGIN      
        1 gcggcaaaat cattcatatc gcggagaaaa atggtgcatt tcagcacatt acccatggtg
       61 gaacccgatt gttcaacaat ggctttgaga ttttccatta cttgacgggt ttcaaccgca
      121 atatctccac cggtatacat ctcgccgttt gcaggattga tggctatttg gccgctgata
      181 aatacaaatc caccggcctc ggtagcctga ctataaggac ctatcggagc aggggcgttg
      241 ggggtataaa tgacgttttt cattgccgat cttgaatttg aacaagaggc aaaagtaagc
      301 attgatgcga ttacggcata gaatgcaact actgtgcggg tttgaggcat ctaatgagtg
      361 tttggaagtt ccaaaagaga gctgtaaatt taacccttat ttttgcctat attgtgtcag
      421 aacccaagca cataccacta atgacccgaa tagttattcg ccaactcctc ttggtgatga
      481 tctttggcgt taccgcacta tcagtaaacg cacagattac ttataccacc ggttggacag
      541 cagattcact ggcagcatac ctagcaggtg ccggcgtgga gatcgataat gctaccatta
      601 actgccataa cctggctatc ggaaaattcg attgcgtgga ttgtaacgtc ggtattgata
      661 gtggagtagt gctgacaaca ggtcgcgttt ccaatatctt aggcccaaat aattcaggaa
      721 gcaccggtac aaataacggt tggcccggcg accctgatct gaatgcttac cccggagtag
      781 gaacaacaca tgatgcctgt gcgcttgaat ttgatgtata cgctccgggc gattcattga
      841 aattcgatta cgtttttggt tctgaggaat atcccgaata tgtgggttcc atcaatgatg
      901 catttgtgtt ttttatttcc ggtccgggtt atcctacact tactaatatt gccttgattc
      961 ccggaacaac attaccggtt actatcgata acgttaatgc tacaagttat ccgatgtact
     1021 atattccgaa tggcaccggc ggtacgggaa tttatgctac atcgccatac tatatcgaat
     1081 acgatggttt tacaaccgtg ttaactgcag aagcagaagt aactccttgt gagtggtatc
     1141 accttaaact agtgattggc gatgaatctg attttgtttg ggattcagga gtatttctaa
     1201 aggccaaaag tttagtcaca aatgtgcttg ccgattttac ctatcccgga gtgccattcg
     1261 gcggcacagc tgaattttgc actacaggta ctgaccctgc tcctctttat gcaacaggtg
     1321 ctaccgcagg aacatttaca tgtacacctt caggtctcgt atttgatgca gcaacaggtg
     1381 ctatcgatgt tagtgcttct gatcccggct cttatactct cacaaatact ttgcttaccg
     1441 gaatttgtta cggtgataca gcaacttggg atatcacagt aaatatttat gaaccggatg
     1501 ctgcaggatt tagttacccg ggatcaccat tctgtgctaa tgaatctgat ccgtcgccaa
     1561 ttcccatgat aggtgcttca ttaggtacct ttaccgcaac acctgccgga cttgtaatta
     1621 acgctgctac cggtaccatt gacctcagcg catcaactcc gggaacatat actatcacca
     1681 ataccgttgc cagcggtact gtttgtcctg atggcgtagc tactaatacg gttacaattt
     1741 atccaactta tgcagtatcg caatctggta atatctgctt aggtgatcca tttattttac
     1801 ctgatggcgc aactgtgtat acagggggaa cttatgttac aactttacct accattcatg
     1861 gttgcgattc tgtaatcact acaaccctgg ctgctcatcc tgtttataat tatacattga
     1921 atccggcaat atgttccgga gctacatata ccttgcctga tggtacagta gtgtcaacaa
     1981 caggtactta tatcaacaat tttacttctg tttggggttg cgattctaat tatactatca
     2041 acttaacagt atcaccggtt cctgttgtga atatgaacgt acacatttgt tccggcgaga
     2101 catatatttt acccgatggc acagcagttg gtgtgtcagg cgtttatcct gtaacgttaa
     2161 ctactgtagc aggttgcgat tcaacaatta atactacttt gtttgttcat cctgtttatg
     2221 caatcacaca aaaccctgtg atttgcgatg atgtaacatt tacacttcct gacggttccg
     2281 ttgtaaatac tacaggtact tatgttacca acttacttac aactaaaggt tgcgattcaa
     2341 taattactac taacctgaca gtgaatccga cttatgatat tatcatgaat ccggaaattt
     2401 gtatcggcga tacttataca atgcctgatg gttctgttac cggaacaggt ggaactacag
     2461 tatttccata cgcatcaatt aatggttgcg attcaattat tactgttaac cttaccgtta
     2521 atccattacc tgtaattacc tggccaattg atgatatttt ctgtttggaa cagaattcaa
     2581 ttgcactcga agctactcct cccggtggta tttacagcgg cccgggaact agtggcgatc
     2641 atttcctgac aaatgatgcc ggcgttggtg gtccatatac cctcacatat gaatataccg
     2701 atgcaaatgg ttgctatagt tcaatgacag ttactacatc tgtagatgat aacttcgtaa
     2761 ctgcctgggg cgataccacg ttgtattacg gatcaagtgc aatactgcat agcgaaagcg
     2821 gtggcgatta tacatggtcg ccaacaaatg gagtagagtg tccgctttgc cctgaatcgt
     2881 atgtgctgcc tccatattca atcgattaca tcatgacatc ggttgatgaa aatggatgcg
     2941 ttgccagcga taatgtgatg ataaccgtat tgccattgcc accaaatgat ttctttgtcc
     3001 caaatacctt tacgccaaat ggtgataata tcaatgatat cttctacatt tttggttata
     3061 acgttttatc cattactgat tttgaaattt tcgaccgctg gggaaaggta gtgtatcgcg
     3121 gagaaaacct tgatgcgaat gatattaatt taggatggga cggtaccatt aacggcgatc
     3181 ctgcaaataa tggcgtgtat gcttattata tcacggtgca attcgataac ggtcagcaaa
     3241 tgaaacgcgc cggtaatatc accctgattc gataattgat tgaataaaat atttaggaag
     3301 caacctgtaa ctcagacaca ggttgctttt ttgtttgtac catgaggaaa taatacaaac
     3361 atagtacact gtgaataggt aatgcaaata atctttttag cgggggcgat gtagtatttc
     3421 gtttgcctga aaaaatttct ctgatacatt tcattccctg taacatccgg aatcttcgat
     3481 gcacatagcg atgcagatat ttatagaaag ctgcagaata agtgccatga tacatcatca
     3541 ataattcatc gctgtcattc caattttgct tgttactcaa ttgcgcaaat acgcgatcgt
     3601 aaaatacagt gcccggtaat ggatatgaaa tggaaatacc aatatcatag ggtttacaat
     3661 tgcgcaaaag ttgcatggtt gactttatat cagtcaatgt ttctcccgga taaccgaatt
     3721 gaagaaaaaa acaaggcttt attccggagc gatacataag gcgactgctt tcggttatct
     3781 gtgctattgt tgtgccttta tccatggcat cgagaatttt ttgcgagccg ctttcggcac
     3841 ctatccacac ttcatcgcaa cctgctccgg caagtgattg gatattattt tcatccaaca
     3901 atagatctgc tctgcattga atcttaaatg gtatcggttg ttcttgcatc aaaccggcaa
     3961 agctgtttat ccaatttggt tttaaaccaa aaatgtcatc gcagaaccaa atatgttcaa
     4021 cctgataggt atttcgcagt aaattaattt catctgcaac agctttagcg cttcgcgaat
     4081 gatatcgatt gccgtaaatc ggtttggcac accaattaca tttaaaggga caaccacgtg
     4141 ttgtggtcat attaatggaa aaatatcctg aatgtgccat ccagcgtgaa cgatattgat
     4201 ccatgtcaat aagatcccac gcaggaagcg gcaaattatc caggtcttgc attaatgcgc
     4261 gtgcaggtgt ttttgcaaaa ccggttgttg tgcgcaatcc taatccctca atggtattta
     4321 aagccggatt ttcatgttgt aaggcgtcta cacatgcagc aattgtttgt tcaggttcac
     4381 cgattacaat ggcatcggct ccaacattta aataatcttg ccaatgatca gtggcgtcac
     4441 tactcgaaac taaaatggta aacccctctt ctttggcctt tttgatcatt gctgagcagg
     4501 cctcccgcat gttgaccaga cacatttttg tcaggtaatt aaatccgtcg tcacaaataa
     4561 tgaatatgcc ggcttgaaat ttggacaatg tattaaagat tgcctccgga ccggaacaaa
     4621 aaatggtatc aaagaaatga atatcatatc ctttttcgcg caacacggca gcagcatata
     4681 gggtgcctaa aggcggatat ggctccatcc tccgctcctg tttcggatcc aggtgtaaaa
     4741 agtaagtatt acaaattact acaggcttca tgcatccaat ggaaaatcat gtttttgtga
     4801 aaactgatgg catcccttat tgaaggcctc taatactttg aattgaaatc cttgggggtg
     4861 gtgctttgaa gcgtgtttgt ttccacgcat gttcacctca aattgtgttt cgttcagata
     4921 gcgatatttt cttcgcagaa aaaactgcat caaactgaaa catatatcat ccatgcggtc
     4981 accgaactta cctgataata gtttctccat gctacgttta aacacaggca tacgagaatt
     5041 ttgcgtaatt aaaatcggca tctcgaaatt aggaaagtag tctgaaaccc aagcgttttc
     5101 ttgtaaaaat tgatggtagt atttagcgcc tgtcacattt tttaatgtta cgatttctgt
     5161 agcggcaaat aaatttttat caggtattag aaattgttct gtgtcaataa agtagttaac
     5221 gcagaaatat ttgcgttgat tcaatagaaa taccttctta aagaaagtta gcatcagtct
     5281 tgttaaccaa atcctgtttt tagatgtgat tacaaagaag tcgacgtcag tatcggcatc
     5341 gaaataattt ttcgacatgc ttccactgat attcacgctg cgaacaaacg ggaatgcctg
     5401 tataagcttt gctcgtttga tggctttggg cattgcctct atcgcaccct tgtttccaca
     5461 tcgccttctg tcggccaaac caattgaatt atgaatggaa taaaggtcgt caaatttgaa
     5521 aattaacaat cgattgcata gtctgtcgag aacacgttca acacttttta aattctcggg
     5581 gacaatgttg cagaatagaa tgatttcttg cttggataat gggtattgaa atatatcgaa
     5641 gtagatcaag gcacgcagaa tagcacgctc aagagttgag gcgttttcaa attgttcttc
     5701 ctttacgata tgcaagttgc tattcatgcg cgaactattt cataaatata gttccattcg
     5761 tagaatattt gtttcttttc tgtcatttct agccgcaaat tgccaaatcc agctaaattt
     5821 tctatctgcc cgataccggt atcttcagat aatatcatat atacttttgt ttgtgggtgc
     5881 ataaatacag gaagttgctc aaacattttt ctgaaatatg catagtcttg cccgcaatac
     5941 caggcgcgtt ccaggtcgtt acgtggatct aatggataaa atggcggatt gattacgatg
     6001 taatcatagg gagcatccgg aatctgttcg aataaatcac tttcaatgat tgttaaccca
     6061 acatgatttg tccgtgcatt atcccgtaat gaccgcaagg cttctttact aatatcactt
     6121 gcacttactt ttgcacccct ttttgccgat gcaatgctga tgaggccatt gccggcacct
     6181 aactctagta attgtttacc tgaaatatgc tgtttaagta tgaattgaag taaatatttg
     6241 gtactataaa agtaaccggg atgaaataca cccggaggta aaaacaaggt aacatcctcc
     6301 catttataaa atgacgaacg ctgcattttc ctccgcacat acggttgaac aaaccaggcc
     6361 gtaaaaaatt tcatcaattt gcgcttcaaa atccttcaat ttcaggttgt cgatatcgca
     6421 gtagtttttg catgagcttt acttctatcg ggaatttgta aaactctgtt ttatatcgcc
     6481 atgatgcaag tgaacgcatg agttttttgt cgaaggattt taatttgata tcgctcaccg
     6541 ttggatatgc gccattcaac acaacttcaa atgacctgat tttgtctatc atatatggtt
     6601 taagccacgg ggtaagcgga ttttttcgta aatcgaagga ttcccatgcc ggtgatatcc
     6661 agtcttcgag ttgttgtgga aaatgaaatc ctcctgcttg cacggctgaa aataattcac
     6721 tgccttctgt cggtaccgga gaatacacat atatgataat ctcagtttgc ggattgatag
     6781 attttatctc ccgaataaat gcgatatctt cttcaatcct ctgcaaggtt tctgcttcgt
     6841 ctttgcccgg ggtgcctaac acaaatgaat attccggaat aatatcaaat ttcttcaaac
     6901 gcgctgcaaa ggctctgatt tgatccgctg tttgtgtgcc tcctttgtcc attaattgta
     6961 aaactgcatc gttgccggtc tctgcgccaa agaatatcat tttgcaaccg gcatcacgca
     7021 tcaattgtag ggtatcatcg ctgtatttgt gtatggtatc aatgcgaccc tctccccacc
     7081 attgcatgtt ttcattaagc atgagtgttg agaaatcagc ggttcttttc tctgatacaa
     7141 agaaattatt atcgtgaaat tcaatagcat cgccaccgta ggtgtttttc agaaattgaa
     7201 tatccttgta aattaattct gctgatctac ccttccaacg cgcattgtaa ataggaacta
     7261 taccacagaa agcacaggtg aaaggacaac caatgctgct atgataggcg attgttttgt
     7321 tgccaagaat tgtctttccc aaataaccgc gcatgtcgta aaaatcatgt aatgcttgat
     7381 acggcaatgc cggtaaggtg tcttgatcat ataattcatc cttgtgattt ttatgaacct
     7441 caccaccgtt tttccaaatc aaatttggaa tcatgtcaag aggcccatcc tgttctagcg
     7501 catctattag catcggaaat gcacgatcac ccggaccata cactatacta tccacccatc
     7561 ccgactgcat cactacgccg gattgattgc ttgcaaaata gcctccccat acaatatata
     7621 tgtcgggaaa aagtgacttg atttgttttg taatgggaat tgcttgtttc agttgcggcc
     7681 cgggcattac agtgcatgca aatacgttga attttccggt ttgcaaatgc tgcataatta
     7741 tggaaagagg atcatcctcg cgattgccat ctacaatgat atagggatat tttccggcaa
     7801 tggcggccgc aattgataga atagaattcg gtatcctggc cttgtgcttc gcagcccgcg
     7861 gattgaacaa taatataccg aattgtgctt cttccttggt catgacatcc cgtacaggtt
     7921 caaatatgca ttaatacatt gttcatcatc aatagaacga acgggagtaa agttaatagg
     7981 atgcaaaagt aattgcatga tttttaacac aaattcgttt ttatccgcac atacaatatg
     8041 cttcggtgaa tctgcaatat aaccaacgtt gtagctgagc acatgcattc cacaaagcag
     8101 cgcctccccg taaacattgc attggccttc aaaacttgat gtatgtacca aaattttaga
     8161 gcgctgcatg tattcgaata cctcatctcg gtttatgctt tccagcacaa caatatgatc
     8221 ttgcagatgc aatgcaacaa cgcgtgaatt tatttcagct aagagtggac ctcctccaat
     8281 cattacagcc ctgacaccgc tgtgtttttt ctttatttcg gcaacaattt caataaattg
     8341 taatggctgt tttacttgaa tatatgatcc tacaaacaat aaatctattg accttgaaat
     8401 aacagggcgt aaatcaatcg agggtaatgg aagcggtatt actgccttta ctacagtttg
     8461 ataatcattt cgaaaaacat tagcctgaaa attgcataaa gatgtaaatg caacatgttt
     8521 tgatactaat ttaaaataag gattacctgc ttttgcatct tgccccatca atgttacaag
     8581 gcatggtaat ttacgtttag ctgcgaacga agaagcactc attgctgcat cgctcaacca
     8641 aaatgcatgg atgacggcag gctccttgcc aacaatactt tttaaacgct ttttggtttt
     8701 aatatgatta ataagttggt aaatgccttt tctgtttttc ccgcctatgg ggtagacttg
     8761 aatcccattc cataaatatg gttttggagt atatggatat tgtgtgctaa cgatattaat
     8821 attttctgcg ggcacctttt ttaataagtg aaggcaataa tcttgcagat atggcaccgc
     8881 agcgctgtcg ttttcatcgg caggaaatcc cgggcttaca agtatcagct tcgtcataca
     8941 gcgaattttc tggtatacca catcatcatt cgcgctaaca tggatttcaa ctttaacaat
     9001 ggtgccttgc tttttgcata gtttgtttca ttcactacgt atcttacagc atgtttatag
     9061 taggattccg gataattgcg cttgaaagtg acatccctgt cgcttgaatt agcccagtcc
     9121 ggttcattga tgatgtcgtt ttgtatttct gcaaataact ctgttcctct gatgggatat
     9181 gcagttgtaa tggtaaataa atccggtaat gcagccttca gatgtttcac ggtttggaca
     9241 atgtccaatt ctgtttcgcc cggatagccc aacataataa aggtgcctgt ttctatacca
     9301 tttgcattgg atagtctgat catttcctgc acactttcca ctttcaccct tcggtccatc
     9361 gcatcaatga tacgctgcga gccactttca gcgccaatcc atactctgaa acatccgctt
     9421 tctttcagta aatgaattac ttcagcattc attctatctg cccgggaaat acattcgtat
     9481 ggcagtgtca aatttgcttc tttaacgcaa cgtgcaaatt cttctaacca tttatgactg
     9541 atcgtaaaca catcatcgac aaaccaaata gtatcgggat tataaacttc ttgaatgtgt
     9601 tgcaactcct ctacaacttt ttgtggtgaa cgccggcggt aactcatgcc atatactgcg
     9661 cgactacacc acttacaagt atatgggcag ccacgcattg tagaaactga aatggcgttt
     9721 ttgccgtgac gcgatttcca tacctccaga tatttttgaa tggagatatg ctgacgtgca
     9781 gggaaaggta aaatgtcaat atcttttatc ttttcacgtt caccggtgaa atgcatattt
     9841 ccgctctcat ccaaaaaata tatgccggca atttgataaa gatctttatg tgttttccag
     9901 cttgtcacta atgtttcaaa agtgatctcc ccttcgccaa caactaatac atctgctccc
     9961 gtttgcaacc attgttgtgc attatttcgc acttccggac cgccaagaat aattttagtg
    10021 tgcttgagat ttatctcttg ctttattttt ttgatgagtt gaacaacatt tttcctggtc
    10081 atcaaattca cataaacacc aaggaattca ggttttgctt cttttatttg attccattgc
    10141 tcgtcttgtg taatgaatgt ggtgtctatc acatgattgt taatgccatg tgcatccagc
    10201 caaccggaaa tgtaaagaat tcctaatggt gcataaggct tcatgatctg ctgctctcgc
    10261 acatcatcaa taattgaata cccatgagtg agtattacct gcatgcaaca aagctattca
    10321 attattcaca gtcaacccaa ggctgacaag gtttatcaat gcagtaaggt tcacccggtc
    10381 gggggattgt gtattttaag gtattattct acttttgact tgtaaatcga aactacatgg
    10441 ctatcaagat tgtcaatgac agtaacttaa aaatgcacac acggtatact accgaacaag
    10501 gaacaggtat ggtgcctctg cttaaaccca taaattttct tgggccaaaa cctaccggta
    10561 caaaaattga tgtacccgta gaagttgcgg acctggcagt gcataatgca accaaaatat
    10621 ttaatgtaac cggcaccgga ttagatggtg aaggcagtgt gttggcggtt ttggatacag
    10681 gaatatatgc cgaacatcct gattttggag ccagagttat tttatccatg aactacaagg
    10741 gcgagaaatt gccaacatca gatattcatg atttacacgg gcatggtaca catgtagccg
    10801 gtttggcagc tgccggtaga agtaaagctc tcgctaaatt ttccggaatt gcaccaaaag
    10861 caaaaattgt atctgtaaaa gttaccgagg gaaatgaaga caccaccacc tggaaacgaa
    10921 tagctgatgg ccttgatcaa gtgttgaact acaatgctga tgccggtact aaaccttata
    10981 ataaggttag ttgtgtgctc atttgcttta atagctttga taatttcaac aacgcaaatg
    11041 aatttgatac aacaaataac aggttgatga agctttttaa aatctgttgt gaaagtaata
    11101 ttgtcgtggt ggtgtcgagt ggcaaccatt ttattgattt tgctgatgtc tcttcaagaa
    11161 caattacaga tggcttcggt aaaaaacata aaacaccaca agttcttgag cataccggac
    11221 ttgcttttcc tgccgtcagt cagcatatta tttgtgcagg cgcaaccgcg aatgatgatg
    11281 gtatttacta cgtaagcaga tcggatttag gcgataccgt ttcttttcaa aaagacgata
    11341 tggctatttt tacacaacgt atccgcaaag aaacaaatga tacatctaac ctttccttat
    11401 ttgttgcagt gcccggattg caaatggtat cattagacaa tcggccgatt cagcacgctg
    11461 tcctaccagg tcaatatcct caaggataca ccgttatgtc gggtacaagc caggctgccg
    11521 gtgtcttagc gggaatggta ttgctattgc agcagaaaat cagaaataag ttgcatatac
    11581 atgaatcttg tgtccccatt caaaaaatat ttaccgcatt gaaaagcacc ggcgaagaaa
    11641 gaaatttcga tggcttcgga cataccgttc aaccaccaac atctacaacg catgcagcaa
    11701 ttggaggcct cacgggtgct cactcaccgt ttaggaattt ccgcattttc gatcttatta
    11761 aggcaattaa ttacattcaa aatcattgat caatggcact tacctttccc actaacatgc
    11821 tcgcaaacaa gccgtacgtt tatttagatg agagcggaac acctttgaaa atagaggtgt
    11881 atgttaaagg tcctcaaacc ggtgctgcat atgcattgca ggcaagcggt gctccacaaa
    11941 attccaatac ttatttgctt accctcacac ttacaactgt tactacagga tcgcaagttg
    12001 ttaaaacaat aattgactgt tcaactttga atggatttag tagtacgaac tttagtaaaa
    12061 ttgttgtgcg tgtggtttat cagaacaata tggttggaag aacaactatt aaaattggtg
    12121 caaatgattt tgatgctgtc gactataaat tgttttcata cttacagttt atcagaactg
    12181 gtacactcaa ataccatggt ttattggaac ttggtcctga tgaattgagt caagtgaata
    12241 tctctcaacc ttcaaatccc gttggtaatc aatctgattt tattatgcga ctaaatgtaa
    12301 atgttgttca ggctaatatt tacacaaatg ccgttacccc gcattttact tatgagattg
    12361 gaagtgccga tggctatgat ccaacaactt atgagacact tgagttgcgt gttgatggtt
    12421 caaaccctaa aaagacaaag ggctcaacca ccgatagcga ggcagatgct tctggtgatg
    12481 actaaaaccg ataagtctct gttgttgctt accttagcag taaacacacc tacatgagat
    12541 caggggcttt ttatttgttc ttgctgctag ttagttattc cttcctgatg aatgcaggca
    12601 ccggtaacga cacagccagg atcaatgcgc ttaacaaaat gtccttcgat cttattaaaa
    12661 aggataccac tcaggccctc gagctagcta aatcagcatt gaatctgtca gagcaaatta
    12721 attacctgca tggcctaggc gatgcctata cgcgattggg actgttggat aaaaatgcgg
    12781 gtaggttgaa tgaagctgta tctaactata aaaaaagtct tcactacagg agtgccgatc
    12841 atgattcaat tggtatagct aagtgctata ctaatcttgg caatgtatac aacgaaaagg
    12901 aaatgattga ttcagcaatt ttttatcaac tttccgcttt gcgtattaat gaaaaagttg
    12961 ctgaaaaggg tgtagtggta gataattata ataatcttgg attgtcttac aaaaataatg
    13021 gtgactatgc gcttgctgaa gaatatctgc tgtcaggata tcaagttgca aaaaaaacaa
    13081 ataacataga agaacagcga ctggcacgta tttgcaataa tcttgctaat gtatattatg
    13141 agcaaggtgc gaatatggca aaagccaaac catatttact ggaaaccatc tctttggctg
    13201 atagtgtttc tgacgatttg ctggctgatg catccaatac actcggcctg atattgatgg
    13261 agcaaaaccc ccttccaaaa gatagtattt tattttattt tcaatttgct tacaatatct
    13321 ataaatccac aggaagcaca gaactcgcag ccgtaaccag caatcttggt gatttttatg
    13381 tgcaaaccgg taatgatgaa ttagcgataa gatacctgct ggagagcaat aaaatttgta
    13441 ctgatcagga attgaaaaca caattgatta caaattataa attactggca acggtttata
    13501 tcaatcaggg aagaactgca gatgcctatg aagccgtttc tcaggcgttg gcattgaatg
    13561 aagaagtgta tacacttgaa aaaacagaaa aaattgcgca gttacaactt gcctacgaaa
    13621 ctgagaaaaa ggaccggatt attgcagaag aaaaagcaac atccgccatg caggcaggtg
    13681 ttattgcccg acaacgcttt gtaggtagcc tgttagcagg aggtacgctg ataattattg
    13741 gtgccatatt gttttatatg cgtcagcggc aactcaaaag aaagctggag tatgaaaaga
    13801 aattagcaga cgacagactg cgcatctctg ccgatttgca tgatgaaata ggttctgcat
    13861 tgagtagtat tggcttatat agtgaagtat tgaaaaacac cctgcaggca tctgactatc
    13921 cggcagcaat taattatggc agcgaaatag ctaatacttc acgagagttg atggataata
    13981 tgagcgacct ggtatgggcc gtaaacccgc gcaacgatag ttttgaacaa cttaccacgc
    14041 gcttgtcgaa tttcggatat aaaatatttg gcgcaggaaa caccaatttt aaagtcgaga
    14101 ttaccggcga tatttcttcc aaagaagtgc ctatggctgt aagacatcaa atttacatga
    14161 tcctcaagga ggctttaaat aacagtgcaa aatatgctca ggcgaagaat gtgctattgt
    14221 cgatcaagta caaacctgat agtatcaatt tgagtgtcac tgatgacggt cgcggatttg
    14281 atattgccaa tgcaggaaat ggcaacggac ttcaaaatat gcgccgacga gcagaaaata
    14341 tagacgcgca gttcgacatc tgctccggga ataccggaac tcaattgaca ttcaatattc
    14401 caataccttg aaatcccccg accgggttat tgaccattga ttcaaattca ttcatctttg
    14461 taaaaactac atcacatgtc tatcaaagta tgcgtttttg atgaccatcg cagagtgcgt
    14521 gacgctatga gtgccctgct gaaaggaaca cccggcttcg aatggggagg cggatttcca
    14581 aatgcggaga acgtggtcaa agacgtagag aaagctgacc cggatgtagt cttgatggat
    14641 attgaaatgc ctggcctgtc gggaatcgat gcagtgaaaa tgctgcatgc gaagtttccg
    14701 gatgtaagaa tcattatgca gacaagtttt gatgaggaca gaaaaatatt ctattccatc
    14761 tgctatggtg ccaatggcta tctgttgaaa aatacccctc cgcatcaggt gttggaagcc
    14821 ataaaggaag tgtatgaagg cggcgcacct atgactccaa gtatagcaag acgtgttgtc
    14881 gaattattca ggtctgctgc tatggaaaaa ccaatggctg aaaaggacta tcagctcagc
    14941 gatcgcgagt ttgaagtatt gcaactcctc gcaaaaggta agagttataa attaattgga
    15001 gaagcattat ttatttctgc cgaaactgtt catagccacg taaaaaagat ctatgataaa
    15061 cttcaggtgg catcaaaaac agaagcagtg gcaaaagcac ttcaggaaaa tttgatcagt
    15121 tagttttatt tatgtcataa atcattaaac cccatggcaa ttcggtcatg gggttttttt
    15181 atcggtcaaa tattttcacc catataggtg attgccgaaa cagctttggc tagtcatctt
    15241 tgcattgtta cagaccagtt acatattcac ccaattaatc cccgaatgct atgaaaggaa
    15301 ttttttatgt tattgtttgc tattgctatt tgattacaaa tgttgatggg caaaccatta
    15361 gttcatttgt aattgcaaca gatggtcaaa ccttcgcggc tcacggcaat atttcatata
    15421 ccattggcga gacttgtata cgcactttcg ataaatatgg atatacgctt gaagagggat
    15481 ttcagcgtgt caccaccatt cccgaaccag ctttggacac aacaattttt gaagcagaca
    15541 atgtgaccgg gctatctcct gaaatagtta tatcctgcta ccccaatccg gctactgcgc
    15601 tgattacaat tgaatgtgat gcgatgctca tttcgggtgc ggaaggaaca atttataatt
    15661 taatgggtgc cgaagtaatg caattcaaat ttgagcatga gtacagcacc caattggatg
    15721 ttacttcttt gtctgcggga caatacttgg tagctatcca aagtccgaag ggatttgtaa
    15781 cggcaaaaca gatattcact atcctaaaat aaataaatat gaaaacatca aagctatttc
    15841 ttgcaatatt tttattgatc ccattatggg gaagctcgca agatgcatta aattatcagg
    15901 cagttatcag aaacccttcc aacgaacttg taataaatac agaaattaaa ttcgagttcg
    15961 ggatcttggt cggtggcagt caggtatatg ctgaacgtca tgctgtttat acagatatga
    16021 atggacaact ttcgctgaag attggcaatg gcttggtgat ctccggtgta tttgattcta
    16081 tcaaatgggg atctgattat atggagatac tcatccgctg cgacattgat ggcgatgata
    16141 tttatgatat catcactacc gaagcattga attctgtgcc ttatgcccat tatgctcaaa
    16201 ctgctgtttc tgcgcagacc agcaaggata ataaatgggc cggaagcggc gaaatgatat
    16261 atactatttc ccctgaaacc aatgtgggta ttggaactac tgctcccatg acaaagcttt
    16321 cggtgaatgg ggctatctcc ctataccctg aacccgatgt aatagtaatc acctatgata
    16381 attatttttt ggaagtaggt gatagaagct atttgcagtt gcagtctgat gacacgttag
    16441 ctgctaacag aacaattatt ttgggtgatg gtatcacacc ggggcagata attattttac
    16501 aaggtttcat gatgtcggac accttccttg aattatcgga tggtgatgcg tcgaatatca
    16561 atataccaca aacgtttatt atcgggaatg atgatgtggt tacattaatc tggagtggaa
    16621 tttcgtgggt aggcgtgtct atctctgata atgaataata aattgatatc gcattcagct
    16681 tgattgtata cggcttatcg aattgttctc ttcgtataaa gtgtatcccg ggttttaatg
    16741 acccgggatt ttaatttcac ccgtttgggc tattgtagcg gctgtaattt cttgattgct
    16801 ttacattgtt gattttacaa ttcattattg actcggccga caaataataa ttgcattgtc
    16861 aatagaatag tttgaagtaa tccgaacact actgctattc cctgaaaatt ttatcaccaa
    16921 taaaactaaa aacatgtcac gtttaaacat tagcggctca gtatcttatc ttgatacaac
    16981 acccgcaaaa aatgctacag ttcgtattat cgatcaggat gatcgctcgt cagataatgt
    17041 aattctgacc gctaccaccg actcgcgtgg aaaatttgtt ggaagaacca ttgattggaa
    17101 agatgaaccg gatggatttt ggccatggga acacgatttc atgaatctca ctttcgaggt
    17161 aaccgattca attggccgac gccatacagg tcctttcatc tatgtaaatg ctgctatcag
    17221 cgttcctatc ctattacctt ttacaaaacc cgccatcaga tctgaaaaca gagcattact
    17281 tgtattggct gacctttcgc aggtgcttac cggaacactt aaaaatttca gaccattata
    17341 ccagttttct gagtcaaatg gtgtaaatct tgtaaatgcc aatttgcgtg gttattacca
    17401 tgaaattaaa gtgcttggac ccggagatgt aacacttgat cgcattgtat caaatctttc
    17461 gcgtatgacg ggtaatagca atatatggga agtggatttg atcgtattcc tccatggtgg
    17521 tcgcgactcg ctgactttga gcaatgatac tgttcacatg acgacgattg taaatgccat
    17581 tcgggatgca aatcttccgg catccaaact tcgcatggta tactccactg catgttatgg
    17641 tgcttctcaa gcagacaatt gggtatcggc aggatttaat tgcgctagcg gatccaaagc
    17701 ctgcaatgcc aacggtgctg tggaattact accattctta ttgaaatggc gtgaaggaaa
    17761 aacatttggc acctgcatta gtgaatcgga aaacctggta ttaagtgcac catttgatgc
    17821 tgcagctaga ttgatgttac ccgaccaagt tgttaattcc gataaagatg tcatcggcga
    17881 tggcactatt accatctctt cgttctaagt gtgttctctt tagaagccgg tcttattgat
    17941 cggcttcatt tttttgtagc gtaatgaaca aatgatcacc tgaattgcga aatagtttta
    18001 gccctccgat tgcagtttca atatttctca aaagcgcaat caacattgta ttatgccaga
    18061 aagctttttt gccatataca ggcgggatac ataatccaat tggtctggag aaaaccagat
    18121 taaaattatc gcccgcaaat tcaactatct cctctatgct gtaataccat acaggtaatg
    18181 agttttcacc tcctgttttg gctattacgg cattttttga ttttctgcgc tttgcttgtt
    18241 tgaaattccc tttcaatgaa aa
//
LOCUS       DAOQOU010000047        17830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1585_length_17830_cov_5.839662, whole genome shotgun sequence.
ACCESSION   DAOQOU010000047 DAOQOU010000000
VERSION     DAOQOU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17830
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1585_length_17830_cov_5.839662"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLM90_05995"
     CDS             complement(<1..264)
                     /locus_tag="PLM90_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88956.1"
                     /translation="MKGGCGIMGIKRVYLLMTCLLPYYIFCQATEEQSKLTGTDIDLV
                     GELIVFDGQLGSLSVHRHDNYIYRDIYFDTPDFDLYNSGYSLRI"
     gene            complement(233..1876)
                     /locus_tag="PLM90_06000"
     CDS             complement(233..1876)
                     /locus_tag="PLM90_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88957.1"
                     /translation="MKAFLLCVVFSSLLINSSKPSIPRGSLILNDHPKEVLRFIDDGY
                     LDLREIRNTCNFLTDPNITIYKGDSIPRRKNAFVRHIKQLNNTLETAWETQTPVNDSL
                     LEILYEIALFESSLKEKILASAHPNIGFDDQLSMWLNTQRSKSVFSVAIDNQINKTPN
                     AKTSPFWHPADSAGALNKRFDQLAKLKKIKAKKKMVVVFDDFSYDGSAPKINTLDLDM
                     DNGWVMKWGDEIHTDVAGSRIFAALGYDVDHPYYYGRDAAMLVFPKWQAVQTSEDLIN
                     MIKQIYHVDLSPFISTIGVIDDAMINDDNNLLPYANCTYMTFIECALEARPDRVKRIG
                     SFIPNNLHNDQRRALRGALLAHAWIGNWDTRESNTLLTQVHMGNYQYRISAVFSDLGT
                     SMGVDLHVVPPDFKTGAVNNFGWEVLRTYGNRIYFNEPMNALLSPYKDAQYADLLWMA
                     NLIAQIDGVALRKMVDEANWPEPLATLYYYKLASRRENILNTFHIKDPHPIQYDIYYS
                     VKENGEYLIENGLLLKEIQPADHPEHFLKHEGRMRNYGN"
     gene            complement(1878..2372)
                     /locus_tag="PLM90_06005"
     CDS             complement(1878..2372)
                     /locus_tag="PLM90_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88958.1"
                     /translation="MSAISEISPLAFALIILISGTSFTCFWWLDSITHGKLAHSDITP
                     QELTTHRIILATSLFMMTSLFLMYWFEPLILLPFFIAGFITRTAHETMDEIHFHINRC
                     TPYESLLHLGMWLSILTNTTALFMWGFFTQYAGIESLSPILAFWALLIGISIIYISKQ
                     EWSR"
     gene            complement(2356..3258)
                     /locus_tag="PLM90_06010"
     CDS             complement(2356..3258)
                     /locus_tag="PLM90_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMZ88959.1"
                     /translation="MFIQPDISPSLPHNTSVLGPKVLELNHVSYRKLHYFHAAALSLF
                     FCGVIISPEQSIIDLSISTWFVLCGVFMIYRFNDIIDHAHGFRFNSKKITGSPIHLLF
                     ILQLICVATPLAWIYLSPFRLTLLSIIGVIGILYSVPLHFRGRVLRIKHTFLLKNISI
                     GVLWGLLPSLGADHITTPLILGLTWFAIMQVTMGSVLRDIADRVHDKLFEVQTLPVRW
                     GVSKTLFYAQIWNILSLTVFICICPEIYIAIAFLTPVIWRAWNIYKIGKSPDAARWTH
                     TINLLTCAVIFIMFIVSQLYVGNL"
     gene            3806..4918
                     /locus_tag="PLM90_06015"
     CDS             3806..4918
                     /locus_tag="PLM90_06015"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMZ88960.1"
                     /translation="MNKKGSVKFVNPDQHLFYQTLKENVNRYFKENGISTHANAQMVT
                     KTIVLFIMYLAPYVLLMTTPMSYGLALGLWSLMGVAFAGIGMSVMHDANHGAYSDSPG
                     VNKMMGYSLNMVGSMVFNWKLQHNILHHTYTNITGLDEDIQAKAGMRFTPNVPLNKPH
                     RWQFIYVFGLYGIMTLYWIIFKDFFQLVRYRKQGVNAASSREYRMQFIKLLAIKAIYF
                     FAIIAAPIIWAGFSFWQVALGFVVMHVVGSFILSLVFQLAHTVEGTDHPTPDKDGIVH
                     NIWAVHQLQTTMNFARKNKVLSWYVGGLNFQVEHHLFPKICHVHYPKIAPIVKQTAEQ
                     FNIPYLENETFWIALRSHLRLLYDLGRIPKLDDIMD"
     gene            5061..5375
                     /locus_tag="PLM90_06020"
     CDS             5061..5375
                     /locus_tag="PLM90_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMZ88961.1"
                     /translation="MNLYVSGLNFKLTSDELRTLFEQYGEVSSAKVITDKFTGKSRGF
                     GFVEMPNDGQKAMDELNGAEIMGKTIAVSEARPQTERKPFNNNRGGGGGNYGGGGGGR
                     RW"
     gene            complement(5451..7187)
                     /locus_tag="PLM90_06025"
     CDS             complement(5451..7187)
                     /locus_tag="PLM90_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88962.1"
                     /translation="MKPICIVFFTAIMLIQQLKAQTPYAVLNPGQTDYNSKFGVYPDH
                     EGNFYLVNKSYYPQDLDAGAGVLTYGPPNQQVLIAKYTSSFELLWSAHIEQATILESK
                     AAYVADDNSLLIAGTTSTDFTIYAATGTSTVSTPSGQPMWLAKFDSDGGLLNAIAIGG
                     PSGSSAPYPVSTSVDNSGNATITGYYTGNVDFDPGVGATTLSGSLSDAYGFIAKYSAT
                     GNLLWAKKIGNGSNTCSVLDHAVDNAGNIILGGWFTQTVDFDLDPFVTHALTAVGSGD
                     AFFAKYDADGNYIWAGQLIENLFYGGVNEIETDAGNNIYVSGFMDKPCDFDIGPAVKT
                     RKPHGTGFYVAKYTSSGALKWIRQPSGNGDFLLNEMYVDPAGNVFTSGTFSGTFDFDF
                     STGTYNLSGPDVDLLITDQWLSCMDKSGNFNYAFSLHTDPTVISGISSIYSVCVTTDN
                     KLIIAGKYLKTLDIDPEVPVWEISIPGVTEAGFVIGYDQPVFRVGDIETSTTLQVYPN
                     PADREITLVLGDVASADISIIDMQGKLIYHTGYNSDEEASIDVSAIPEGIYILRAKTN
                     DQIKSSTICIAH"
     gene            complement(7256..8158)
                     /locus_tag="PLM90_06030"
     CDS             complement(7256..8158)
                     /locus_tag="PLM90_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HMZ88963.1"
                     /translation="MQLHSHISKHKLPILLVAGICLLSACYTNKLSKLAPTEQYPDDT
                     WLSQQTNKRALIIVAHDDDALGCAGTITQLCEQGWQVSMFCLYPDISRVADRNAQRQI
                     DIKNAIAIQGIQDIRWHEFIYRKDLETSQTAYYPVPESSFDTLFFTDTLLSLINAHIE
                     DWKPSVIFTLDDQIGGYGHPEHKYISMLALRCAKEHQLAKGGTVERVYQAVFSPSQAE
                     TSLKDSEVYQAAKEVYHCQGMPIPTTQINIQSAADKKYRAMQAYTTEQPNIMRVWLFY
                     NKVDADTYFNIFDREFFRVIETGE"
     gene            complement(8189..8725)
                     /locus_tag="PLM90_06035"
     CDS             complement(8189..8725)
                     /locus_tag="PLM90_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ88964.1"
                     /translation="MQILIIILAAIAGVVALIFIIASFSSSNFQIVAQLPVKRNRADI
                     YNYVKFLRNSDYFNKWVMMDPGMEKKYTGTDATVGFTYKWKSENKQLGEGEQEIIGME
                     ENSYISYVIRFIKPFENTSQSTILLTDLEPGTTMVSYTFTGQRNLMMKVVHLLFKLEK
                     ALQKDLMESLNNLKKVLE"
     gene            9304..10170
                     /locus_tag="PLM90_06040"
     CDS             9304..10170
                     /locus_tag="PLM90_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88965.1"
                     /translation="MKVRWDIDQIDEYTRKKQFSQLEEYKDNCAAFSNSFTEPVSERW
                     TMHDTMEYHTFKELREMYQAGETNVIVVALARIKITKGADDKKQFDLSYSYYGDEDAK
                     DSSGFDPNDFKALVVFPIEQIMELPDAFKKIYVGVQLEHQNPSKEELYYAVDVVTDLV
                     KRKAADNTFTDKVIKQSAPKLKDYTLAISKDDWDEDYKIKDAEPYYPLPIEVMEPAKF
                     KQLITEGKPGYAYAIKVGLNTTIMLSDKREVAYLQQPNDIPTRKASDKFANRDFHKIY
                     EAVTGVKVEFKE"
     gene            complement(10433..13081)
                     /gene="cphA"
                     /locus_tag="PLM90_06045"
     CDS             complement(10433..13081)
                     /gene="cphA"
                     /locus_tag="PLM90_06045"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMZ88966.1"
                     /translation="MKILSTAVMKGPNYWSIRRHKLIVMKLDLEELEEMPTNKIHGFR
                     ERLEQIIPSLYEHRCSEDHAGGFFKRVDEGTWMGHVVEHIALELQTLAGLDTGFGRTR
                     STSTKGVYNVVYSYLEEQTGIYAGKIAVELCESLVRGEFYDIAPHIMKMKEIRERERL
                     GPSTGSIVEEAIKRDIPYIRLNRRSLVQLGWGKNQRRIQATVASTTSNIAVEIACDKE
                     ETKNLLQQLNIPVAKGGSAYDEDDLEAIIKRIGYPIVIKPIDGNHGRGSTMNIKNWEE
                     AVAALKKSKEVSRWAIVEQYIVGYDYRLLVINYQFVSAALRKPAAIIGDGVHTVKQLV
                     DMVNADPRRGYGHENVLTSIKIDDHTLRLLEKLGMTEESIPSPGEEIFLKSTANLSTG
                     GTATDVTDIVHPYNVFTAERIARTIGLDICGIDIMSPDISVPMTENRGVVLEVNAAPG
                     FRMHLAPSEGIGRNVAEPVVDMLFPAGTQGRIPIVAITGTNGKTTTTRLCAHIAKTMG
                     HKVGFTTSDGIYIQNRMLEKGDCTGPVSTKFVLMDPTVDYAVLECARGGLLKAGLGFD
                     KCDYAIVTNVTADHLGLKDIDTVEEMAKVKSVLPETVHKNGYAILNADDDLVFNMRKN
                     LECKIALFSMDENNSRIMEHCERGGICAVLENGWITILKGNWKLRVEKVVNIPLTFDG
                     KATFMIQNILPATLCAYLQGFKVEEIKLALQTFIPGPATTPGRMNIFKFRNFEVLVDF
                     AHNPDGFQAISNYLSKVNAPIKVGLIAAAGDRRDEDIRELGRIAARTFDEIVIRQDKN
                     LRGRTDEEIMNLLMDGILEIKPGLDVIRINPEKESIKYVIENAKPGSHITICSDVIAE
                     ALDLVLQYKEKDDQFEFNKGEIPNVH"
     gene            13276..14091
                     /locus_tag="PLM90_06050"
     CDS             13276..14091
                     /locus_tag="PLM90_06050"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HMZ88967.1"
                     /translation="MIPKGRLIAVGGAEHRDHIEQDKLEVLRRILQEMRGPDTVLEII
                     PTASGIPKQIAREYTDAFGTLGCKATRIMNIRKQKDAQKKEFLDRLRSCDGIMFSGGN
                     QTLLSETFLGTEFLDILKSRYINEPGFVIAGTSAGAMAQSGSMINGGAPAEALMRGKA
                     LMIEGLGFTNNSIIDSHFVNRGRFGRLMVAVAEHPQLTGIGISEDTAVIIKEDRYLEI
                     IGTGLVVIMDGTELRLNNIANNKGEMVNLERMIFHLLSKGRHYDMQERKVITN"
     gene            complement(14088..15011)
                     /locus_tag="PLM90_06055"
     CDS             complement(14088..15011)
                     /locus_tag="PLM90_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HMZ88968.1"
                     /translation="MNAPIRIAIHGGAGTMPKRLMTPEIEAACHDSLQLALQAGYSAM
                     AKGGTAVDGVEAAVRALENAPMFNAGKGSVFTNKGQHEMDASIMNGETLDCGAVAGVS
                     NIKNPVTLARCVMDNSEHVLLLNDGAEEFARLHNIVFEDDDYFFTQLRYDQWQKALMK
                     KITVLDHVDDKKFGTVGAVAIDAKGNLAAATSTGGMTNKLYGRVGDSPLIGSGTYANN
                     ASVAVSCTGHGEFFIRQVVAYDLHCLMTYKGMGLEEAANHIVHDKLKPIEAEGGLIAI
                     DAKGNICLPFNTPGMYRGWMKSEADYFTGIY"
     gene            15157..15858
                     /gene="msrA"
                     /locus_tag="PLM90_06060"
     CDS             15157..15858
                     /gene="msrA"
                     /locus_tag="PLM90_06060"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMZ88969.1"
                     /translation="MKGTKTITAALLSLVLGITGCGAKQATSDNQQIQDNKQSNDLAM
                     RKTYTDEEIRNNGWEKATFGGGCFWCTEAIFEEVKGVKSVVSGYAGGKMEKPTYEAVC
                     TDTTGHAECVQVVFDPKECAFATILDVHMKTHDPTTINRQGNDVGIQYRSVVFYENDA
                     QKTATEDYINKLNASGYYSDKIVTEVVPLTTFWPAETYHQDYFAKNPDQPYCVYVVAK
                     KVDKFEHLFPELIEK"
     gene            complement(15867..16706)
                     /locus_tag="PLM90_06065"
     CDS             complement(15867..16706)
                     /locus_tag="PLM90_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88970.1"
                     /translation="MRIIILHLILCITSSFVNAQSITLGPVEANDTSSLTTADDKLQE
                     GNYYKYFSINLKAGDGLLVYMESSSFIPFVTLINEDTTGFVQGWIEQIKSDVFGSYIN
                     FTTLADTTVFILLTSKESYQTGTFRYVVKQFSGDQLFYDKQMPDCRKLVYLLNQWNAD
                     WKMFPITTVLNEETYEEVSTFENTFFNGTSGRLIRGKYTEVYSELASEALAISQFDSL
                     TTTFSECLLNSNFNHSSSTDGPVKTEWFTINGFSNSTHAASFSLQLDLSNESAPKMIL
                     TLY"
     gene            complement(16719..17108)
                     /locus_tag="PLM90_06070"
     CDS             complement(16719..17108)
                     /locus_tag="PLM90_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMZ88971.1"
                     /translation="MPEHVTKTRIDKWLWAVRLYKTRSLATEAVKNGKVKVNGDSVKP
                     SFEVFPGLILTIPKGVVKYQYKVLAPLERRVGAPLVKDYMEDVTPEEEKMKLTTLEFM
                     PTAFREKGTGRPTKKERRDLDDWMETE"
     gene            17176..17523
                     /locus_tag="PLM90_06075"
     CDS             17176..17523
                     /locus_tag="PLM90_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HMZ88972.1"
                     /translation="MQKPISAREIVLSLKDRFRPEKAEAGYETVFHLDISGDRGGQFT
                     VTIANGTINIEDGFHGNPKCIVTTKDEVYEDTEWGRSNPQMAVMFGKIKVSDLGEILD
                     FIPLFHRCEEYYQ"
     gene            17556..>17830
                     /locus_tag="PLM90_06080"
     CDS             17556..>17830
                     /locus_tag="PLM90_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88973.1"
                     /translation="MRKFLFTALCAFSALTLAAQNFNGLSVSTGNMYPDGSNYGVVLQ
                     NELIQVQQLDTTVKYDVVYEFKNNTSTFATVTAALPVNVYFNEFAPG"
BASE COUNT         5203 a         3574 c         3768 g         5285 t
ORIGIN      
        1 aatgcgcaaa gaataaccac tgttgtataa atcgaaatca ggggtgtcaa aatagatatc
       61 ccggtaaata taattgtcgt gtctatgtac agagagactg cccagttgcc catcgaaaac
      121 aatcagttcg ccaacaaggt caatgtctgt gccggtgagt ttactctgct cttcggtagc
      181 ctgacaaaag atataatagg ggagcaggca tgtcatcaat aggtaaacac gtttaattcc
      241 cataattccg catcctccct tcatgcttta gaaaatgttc aggatggtcg gcaggttgaa
      301 tttctttcag caataaacca ttttcaatga ggtactcacc attttccttt acactataat
      361 aaatatcgta ttgaatagga tgaggatctt tgatatgaaa tgtatttaaa atattttctc
      421 ttcgcgatgc aagtttatag taataaagtg tagcgagagg ctccggccaa tttgcttcgt
      481 cgaccatttt tcgaagtgct acaccatcta tctgtgcaat caaatttgcc atccacagaa
      541 ggtcggcata ttgcgcatct ttgtatggcg ataataatgc attcatcggt tcattaaaat
      601 aaatacggtt gccataggtg cgcaatactt cccacccaaa attatttacc gctcctgttt
      661 taaaatcagg aggaactaca tgtaagtcca ctcccatgct tgtacccaaa tcagaaaaga
      721 ctgcagaaat ccgatattga taattgccca tatgtacctg agtgagcaaa gtattcgatt
      781 cgcgcgtatc ccaattacct atccatgcat gtgccagcaa tgcacctcgc agggcacggc
      841 gttggtcgtt atgcaagtta ttgggaatga atgatcctat gcgtttaacc ctgtcgggac
      901 gagcctccaa tgcacattcg ataaaggtca tataggtgca atttgcatat ggtaataaat
      961 tattgtcatc attgatcatt gcatcatcaa tcacgccaat tgtactaatg aatggcgata
     1021 gatcaacatg ataaatttgc ttgatcatgt tgatcaaatc ttcactcgtt tgtaccgctt
     1081 gccattttgg gaatacgagc atggcagcat cgcgaccgta gtaataagga tgatctacat
     1141 cgtagcccaa cgctgcaaaa atgcgcgagc ctgcaacatc agtatgaatc tcatcacccc
     1201 atttcattac ccatccattg tccatgtcga ggtctaaggt gttgatttta ggagctgatc
     1261 cgtcgtagct gaagtcgtca aacacaacca ccatcttttt tttggctttt atttttttca
     1321 atttcgccag ttgatcaaag cgtttattca atgcaccggc agaatctgca gggtgccaga
     1381 atggtgaggt tttggcattt ggtgttttgt ttatttggtt atctatagct actgaaaaga
     1441 ctgatttact gcgttgtgta ttcagccaca tcgataattg atcgtcaaat ccgatatttg
     1501 gatgtgcaga agctaagatt ttctctttta aactcgattc gaagagtgca atctcatata
     1561 atatctcgag caatgagtca ttaacaggcg tttgtgtttc ccatgcagtt tcgagtgtat
     1621 tatttaattg tttgatatgt ctgacaaagg cattttttct tcgcggaata ctatctccct
     1681 tgtagatggt aatattcggg tcagtaagga aattacatgt gtttctgatt tcccggaggt
     1741 cgagatagcc gtcatcaata aatcgtaaaa cctcttttgg atgatcgtta aggataagac
     1801 ttccgcgtgg gatggaaggt tttgatgagt taatcaacaa agaagaaaaa acgacacaaa
     1861 gtaaaaatgc cttcatgcta cctgctccat tcctgtttgc tgatataaat gatggaaatt
     1921 ccaatcaata gcgcccagaa tgccaatatt ggcgaaagcg attcgattcc tgcatattgc
     1981 gtgaaaaatc cccacatgaa aagcgcggta gtatttgtga gaatactcag ccacatgcca
     2041 agatgcaata atgattcata aggtgtgcag cgattgatat gaaaatgtat ttcgtccatt
     2101 gtttcatggg cagtccgggt gataaatcct gcgatgaaaa aaggcagcag tatcagtggc
     2161 tcaaaccaat acatcagaaa aagtgatgtc atcatgaaca aggaagtagc caaaatgata
     2221 cgatgcgtag tgagttcttg cggagtgata tcactgtgtg ccagtttgcc atgtgtaatg
     2281 ctgtcgagcc accagaaaca ggtaaaactc gttccggata tcagaataat cagcgcgaat
     2341 gccaggggtg atatttcaga gattgccgac atacaattga ctgacaataa acatgatgaa
     2401 aattacagca caagtgagta aattaatagt gtgtgtccat cttgcggcat caggtgattt
     2461 tccaatttta taaatattcc atgcacgcca aattaccggt gttaaaaagg caatggcaat
     2521 atatatttca ggacatatgc aaatgaacac ggtcaatgaa agtatattcc agatttgtgc
     2581 ataaaataaa gttttcgata caccccatcg caccggtaat gtttgcactt caaatagttt
     2641 gtcatgcact ctgtcggcaa tatcgcgcag cacactaccc atggtaacct gcatgatagc
     2701 aaaccatgta aggccaagta tcaatggtgt tgtgatatga tcggcaccaa gagacgggag
     2761 cagtccccat agcacaccaa tgctgatatt ctttagtaaa aaagtatgtt taatacgcaa
     2821 cacccgacct ctaaaatgca gaggaacaga ataaagtata ccaatcacac ctataatcga
     2881 caaaagtgta agtcggaagg gtgataaata aatccaggcc agtggagtgg ctacacaaat
     2941 taattgtaat ataaacagca aatgtattgg ggatcctgta atctttttac tgttgaagcg
     3001 aaagccatgg gcatggtcga taatgtcatt gaagcgataa atcatgaaaa ctccgcaaag
     3061 cacaaaccag gttgatatgc tcaggtcaat tattgattgc tcgggtgaaa tgataacacc
     3121 acaaaaaaat agcgacaatg ccgcggcatg gaaataatga agctttcggt agctgacatg
     3181 attcaactcc agcaccttag gacctaatac agaggtgttg tgtggtaagg aaggggagat
     3241 atctggctgg atgaacataa agtgctaccg taaagatact ttggagggcg gataggatgt
     3301 atgaaaattt cataaaaagg aagcaggcat ttgcgaaaat caataaaata gaaaacgctg
     3361 caccggctcc ggactgtgaa acattttgat tgaccttttg ggtttcatct tcgcagcaga
     3421 catgaggagc ggacatggcg gggcggtgtg gagcatgagc ggcgattgaa tggtttggca
     3481 gtcggcagca tgcgtatatg atgtattctt acaaggcgtt tatgtggaaa actgctgcag
     3541 tttaaggtca attcaacctg ccaaagaccc tgatagattc gattgacaat ggaaacttgg
     3601 tggtattctg agattttgaa aagtcttcag aataaagggt ttttgcgaaa tagaacacgg
     3661 attcgcagga atagtcgagt gcagtaggcg ggtggattga taaaagtcat tggcttaaaa
     3721 gggctacatg tttgcagatt tcagcggttt aagggtcaaa agccatatct ttgcggtaat
     3781 attcgtttaa cagccaatta atctgatgaa taaaaagggc tctgtaaagt ttgtaaatcc
     3841 ggatcagcat ctgttttatc aaacattgaa agaaaacgta aatcgttatt ttaaggaaaa
     3901 cggaatttcc acacatgcta atgcgcagat ggttactaaa accatcgtgt tattcatcat
     3961 gtatttagcg ccatatgtat tgcttatgac cactccaatg tcatacggac tggcattagg
     4021 attatggagt ttaatgggtg tggcgtttgc cggtatcggt atgagtgtga tgcacgatgc
     4081 caatcatgga gcttatagcg atagtcccgg tgtaaataaa atgatgggtt actcgttgaa
     4141 tatggtgggt agcatggtat tcaattggaa gttacaacat aatattcttc accatactta
     4201 taccaatatc accgggctgg atgaagacat acaagcaaag gcgggaatgc gtttcacgcc
     4261 taatgtgcca ttaaataagc cacatcgctg gcagtttatt tatgtatttg gattgtatgg
     4321 catcatgact ttgtattgga tcattttcaa agactttttt cagttagtgc gatatcgcaa
     4381 acagggtgta aacgctgcat catcgagaga ataccgcatg cagtttatta aattgctggc
     4441 tataaaagcg atatattttt tcgccatcat tgcagcaccc atcatttggg caggtttcag
     4501 tttctggcag gttgctctgg gatttgtagt gatgcatgtg gttgggagtt tcattttgtc
     4561 gctcgtgttt caattggcgc ataccgttga gggtactgat cacccaacac ctgataaaga
     4621 tggcatcgtt cataatatct gggccgtgca tcagttgcag acaaccatga attttgcgcg
     4681 caaaaacaag gtccttagct ggtatgtagg gggattgaat tttcaggtag aacaccatct
     4741 attccccaag atttgccatg tgcattaccc caaaatagcc cctattgtga agcaaacggc
     4801 agagcagttc aatattcctt atctcgagaa tgaaaccttt tggattgcac tgagatccca
     4861 tcttcgcctg ctttacgacc tgggacgcat ccctaaattg gatgatatca tggattaaag
     4921 gggtaggggc ttgttttata agttgggatg ttataccttt gacctaatct tgggctatct
     4981 tcttcctttt accggattct tcggttaagt taagcgggca ctcccggagt attttgttaa
     5041 cattttttta aaagaaaaac atgaatttgt acgtttccgg tttgaatttc aaactgacca
     5101 gcgatgaact gcgcaccttg ttcgaacagt atggtgaagt atcatctgca aaagtgatca
     5161 ctgataagtt tacaggtaaa tcacgtggct tcggcttcgt tgaaatgcct aacgacggtc
     5221 agaaagccat ggatgaactc aatggcgcag agatcatggg taaaactatt gctgtttctg
     5281 aggctcgtcc tcaaactgag cgcaaaccgt ttaacaacaa ccgcggtggc ggtggtggta
     5341 actacggcgg aggcggcggt ggccgtcgct ggtaatcagc ataagctttt caaaaaaaag
     5401 gcagttgtta attcaactgc cttttttgtt ttgtgcgaat cgcagaagta ttaatgcgca
     5461 atgcaaatag tgcttgattt aatttgatca ttcgtctttg cacgtaggat ataaattccc
     5521 tccggaatag ctgatacatc tatgcttgct tcttcatcac tgttataacc tgtatgatat
     5581 atcaatttac cttgcatatc tataatcgaa atgtctgcag aagctacatc acccaacacc
     5641 aaggttattt ctctatctgc agggttagga taaacttgta atgttgttga agtttctata
     5701 tcaccaactc tgaataccgg ttggtcatat ccaatcacaa atccggcttc ggttacgccg
     5761 ggtatgctga tttcccacac cggtacttcg ggatctatat ctaaggtttt taaatatttt
     5821 cctgcaatga tcaatttgtt gtctgttgtt acacaaacgc tgtatataga ggatattcct
     5881 gagattaccg ttgggtctgt gtgcaacgaa aaagcataat taaaatttcc tgatttatcc
     5941 atgcacgaca accattggtc ggtgatcaga agatctacat ctggtccgga taaattatat
     6001 gtacctgtgc tgaaatcaaa atcaaaagtt ccggaaaaag tacctgaagt gaatacatta
     6061 cctgcaggat cgacatacat ttcattcaac aaaaaatcgc cattgcctga tggttgtctg
     6121 atccatttca aggcaccgga agaagtatat tttgcaacat aaaatcctgt tccatgcggc
     6181 tttcgggttt taactgcggg accgatatca aaatcgcagg gcttatccat aaatcctgat
     6241 acataaatat tgtttcctgc atcagtttca atttcattta cgccaccata aaacaaattt
     6301 tcaatcagtt gacctgccca tatgtaattt ccatcagcat catattttgc aaaaaatgca
     6361 tcgcctgagc cgacggcggt aagtgcatgg gtaacaaatg gatcaagatc aaaatctacg
     6421 gtttgagtaa accaacctcc caaaataatg ttaccggcat tgtcaacggc atgatctaat
     6481 actgaacatg tattcgatcc gtttccaatt tttttcgccc acaataaatt acctgttgcg
     6541 ctatattttg caataaatcc ataggcatca ctcagacttc cacttagtgt ggttgcccca
     6601 actcccggat cgaagtccac attgccggtg taataccctg taatggttgc attaccgcta
     6661 ttgtcgacac tggttgaaac aggatagggg gcgctggaac cgcttggacc accaatagca
     6721 atcgcattga gcaatccgcc atcgctatca aattttgcta accacatagg ctggccggaa
     6781 ggagttgaaa cagttgatgt gcctgtggct gcgtaaatgg taaaatcagt tgatgtagtt
     6841 cctgctatca aaaggctgtt atcatctgca acatacgctg cttttgattc gagaatagta
     6901 gcctgttcga tatgcgccga ccagagtaat tcgaaactgc ttgtatactt ggcaataagt
     6961 acttgctggt ttggaggtcc gtaagtgagt acaccggcac ctgcatccag gtcttgcgga
     7021 taatagcttt tattgactag atagaagttt ccttcatggt cgggatatac cccaaatttg
     7081 ctgttgtaat cagtctgccc cggattcaac actgcataag gcgtttgggc tttaagctgt
     7141 tgaattaaca tgatggcagt gaagaaaacg atgcatatcg gtttcataag gtgtgtattt
     7201 gtattgaaaa ttacaccttt ttatatgctg atggaaattt ttgtaagatt attatttatt
     7261 ccccggtttc aatcaccctg aaaaattcgc gatcgaaaat gttaaaatag gtgtcggcat
     7321 ccaccttatt gtaaaataac caaacgcgca taatattagg ctgttcggtg gtatatgcct
     7381 gcatagcacg atatttttta tcggctgcgg attggatatt aatttgagtg gtaggaatag
     7441 gcataccctg acaatgataa acttctttag cagcttgata aacctcactg tcttttaacg
     7501 atgtttctgc ctgcgatggt gaaaaaactg cctggtaaac gcgctctacc gtgccacctt
     7561 ttgcaagttg atgttccttt gcacaacgca atgccagcat gctgatgtat ttatgttccg
     7621 gatgaccgta gccaccgatc tgatcatcta aagtgaaaat tacggaaggt ttccaatctt
     7681 caatatgtgc attgatcaat gataataatg tatcagtaaa aaataaagta tcaaatgaag
     7741 attccggaac aggataatag gctgtctgac ttgtttcaag atccttgcga tatataaatt
     7801 catgccacct gatatcttga atgccctgga tggcgatagc attttttata tcaatttgcc
     7861 gctgtgcatt tcgatctgcg actctggaaa tgtcgggata caaacaaaac atgcttactt
     7921 gccagccttg ttcgcaaagt tgagtgatgg tgcctgcgca acctaaggca tcatcgtcgt
     7981 gtgctacaat aattaatgca cgtttatttg tttgttgcga taaccaggtg tcgtcaggat
     8041 attgctctgt aggcgcaagt ttgcttaatt tattggtata gcaggcactt aataaacaga
     8101 tgccggcaac aagcaaaatc ggtaatttgt gtttagaaat gtgtgaatgc aactgcaagc
     8161 tttatttttt tgagatttac aatgttgcct attccaacac tttcttcaaa ttattcaatg
     8221 attccatcaa gtctttttgc aatgcttttt ccagtttaaa gagcaagtgt actactttca
     8281 tcataagatt tctttggccg gtaaatgtat agcttaccat tgtagttccg ggttcgagat
     8341 cagttagcag gatggtagat tgcgaagtat tttcaaaagg tttaataaat cggatcacat
     8401 agctgatata gctattttcc tccataccga tgatctcttg ttcaccttcg ccaagttgtt
     8461 tgttttcact tttccatttg taggtgaatc caaccgttgc gtcagtgccg gtgtattttt
     8521 tttccattcc aggatccatc attacccatt tattaaaata atcgctgttg cgaagaaatt
     8581 tcacataatt gtaaatgtct gccctattgc gttttacagg taattgagca acaatctgga
     8641 agtttgaaga tgagaaggag gcaatgataa agataagcgc aacaacgcct gctattgctg
     8701 caagaatgat tattagaatt tgcatgactt tgagtatttg aataccaaag gtaagcaatc
     8761 ggatgaaaaa aaagcagcat gagtgatatg cggctttttg tatggaatat gctattttgg
     8821 attctcttag cttgtgtaaa gattatcagc tgagtttaag attattatcc ttttatcatt
     8881 aaatattctt acatctcgct ttagcatgta agctatgaag tgatagtgtt caccggtcaa
     8941 aaatgctttg agcctttcgg ttggaatccc attggatttg aagtaaagta ttatattccg
     9001 ggtattaaat ttggcatatt taggcgcgtg tttagcgttt tttaggttaa atgaagcaat
     9061 ttagcgtgga aattgtccaa catcggcaaa ttatttctct ttgtgatatt caaatgaaaa
     9121 cctaattttg aaccaaacag caagactatg cgcatcggaa gatttttacc cttactattg
     9181 atccttacct tttttcatta taacaccgca aatgcccagt tcggtagccc tctgaccgtt
     9241 tttaaaaacg atctgcccac ccaaaaggaa tgtgccgaga taacaggccg aaaactggtt
     9301 gttatgaagg tacgctggga tattgaccag atcgacgagt atacgcggaa aaaacaattc
     9361 agtcagcttg aggaatacaa agacaactgt gcagctttca gcaatagttt cacagaaccg
     9421 gtaagtgaaa ggtggactat gcatgatact atggaatatc acacattcaa agagttgcgc
     9481 gaaatgtatc aagccgggga gacaaacgta attgttgttg cattggcgcg tataaaaatc
     9541 acaaaaggtg ctgatgataa aaagcagttt gatttaagct attcatatta tggtgatgaa
     9601 gatgccaagg attcctcggg ttttgaccca aacgatttta aagcactagt tgtatttcca
     9661 atcgaacaaa ttatggaatt acctgatgct ttcaaaaaga tttatgtagg cgtgcaattg
     9721 gaacatcaaa acccttcgaa ggaggaattg tattatgcgg ttgatgtagt tactgatctt
     9781 gtaaaacgca aagccgctga caatacattt accgataagg tgatcaagca atcggctccg
     9841 aaattgaaag attataccct tgccatttca aaagatgatt gggatgagga ttataaaata
     9901 aaagatgcag aaccgtatta tccgctgcca atcgaagtaa tggagcctgc aaaattcaag
     9961 caattaatta ctgaaggaaa accaggctac gcttatgcga ttaaggtagg actcaataca
    10021 accatcatgc tttccgataa acgcgaagta gcatatctgc aacaacctaa cgatatccca
    10081 acacgcaaag cctccgacaa attcgccaac cgtgattttc ataaaatata tgaggcggtg
    10141 acaggggtga aggtggagtt taaggaatga taaaggaaag aggaaagagg aaagaggaga
    10201 gaggagagag gggtgagtgg gtgagtgggt gagtgggtca gtgggtgagt gggtgaggtt
    10261 accgcgcaga gaaggcggct gggtcagtag gaaaataaaa tgggctaaga caaagtctta
    10321 gcccatttta ttttaaaaaa aaatcccgac cgcaggtcgg gaaaaatact tgatacttga
    10381 tacttgatac ttgatacttg ataccactca ccactcacca ctcaccacta aatcaatgca
    10441 cattcggtat ttccccttta ttaaattcaa actgatcatc tttttctttg tattgtaata
    10501 caagatccag agcttcggcg atgacgtcgg agcagatggt gatatgtgat cccggtttgg
    10561 cgttttcaat cacatattta atactttcct tttcaggatt tattctaatc acatccagtc
    10621 cgggttttat ttcaagtatg ccgtccatta atagattcat aatttcttca tcggttcttc
    10681 cccttagatt tttatcctga cgaataacta tctcatcaaa ggtgcgcgca gcaatgcgtc
    10741 cgagttcacg aatatcttca tctcgtctgt cgccggcggc tgcaattaag cccactttaa
    10801 ttggtgcatt tactttgctg agataattgc tgatagcctg gaacccatca ggattgtgtg
    10861 caaaatctac caacacttca aaatttctga atttaaaaat attcatacgt ccgggagtgg
    10921 ttgcaggacc cggaataaat gtttgaagtg caagcttaat ttcttccact ttaaatcctt
    10981 gcaggtaagc acaaagggta gcaggaagaa tattctgaat catgaaggta gccttgccat
    11041 cgaacgtgag tggaatattc acaacttttt ctacacgcag tttccaattt cctttcaaaa
    11101 ttgtaatcca tccattttcc agtaccgcac aaatgcctcc gcgttcgcaa tgttccatga
    11161 tgcgcgaatt attttcatcc atactgaaca atgcgatttt acattccaga tttttacgca
    11221 tattgaaaac gagatcgtca tcagcattta aaattgcgta tccgttttta tgaactgttt
    11281 ccggtaatac agattttacc tttgccattt cttcaacggt atcaatatct ttcaatccaa
    11341 ggtgatctgc agttacgttg gtgacaatag cataatcaca tttatcaaat cccaatcccg
    11401 cttttaataa tccgccacgt gcacattcca acaccgcata gtcaactgtt ggatccatta
    11461 atacaaattt agtacttaca ggaccggtgc aatcgccttt ttctaacatg cgattttgga
    11521 tatagatacc atcgctcgtt gtgaagccaa ccttatgtcc catcgttttt gcaatatgcg
    11581 cacaaagccg ggtggttgta gttttaccat ttgttcccgt gatggctaca attggtatgc
    11641 gaccttgcgt gccggcaggg aaaagcatat caacaacggg ttctgcaacg tttctgccaa
    11701 taccttcgct tggagcaagg tgcatgcgga atccgggtgc tgcatttact tctaaaacaa
    11761 caccgcgatt ttcagtcatt ggtacagaaa tatccggact cataatatct ataccacaaa
    11821 tatcgagacc gatagttctt gcaatacgtt ctgccgtaaa tacattgtaa ggatgtacaa
    11881 tatcagtcac atctgtggca gttccacctg tgctgagatt tgctgttgat tttaaaaata
    11941 tttcttcgcc gggtgaaggg atactttcct ccgtcatccc caatttttca agcaaacgaa
    12001 gtgtatgatc atcaatttta atgctggtta atacgttttc atgaccgtag cctcggcgcg
    12061 gatcggcatt taccatatct accaattgtt tgacggtatg cacgccatca ccaataattg
    12121 cagccggttt gcgaagtgcg gcagaaacaa actggtagtt gattacgagt aaacgataat
    12181 catatcctac aatatactgt tcaacgatag cccatcttga aacctctttc gattttttta
    12241 aggcagcaac tgcttcctcc cagtttttaa tattcatggt agacccgcgt ccatgattac
    12301 catcgatagg tttaatcaca atcgggtagc ctattctttt aatgattgct tccagatcat
    12361 cctcatcata ggcacttcca ccttttgcta ccggaatatt taattgttgt aatagatttt
    12421 ttgtttcttc tttatcacaa gcaatttcaa cagctatatt agaggtagta ctggcaacgg
    12481 tagcctgtat acgtcgttga tttttgcccc atcccaattg gacaagtgat cgtctattaa
    12541 gtcttatata gggtatatcg cgcttgatag cctcttcgac gatacttccg gtggatggtc
    12601 caaggcgttc ccgctcccgg atctctttca ttttcatgat atgcggagcg atgtcgtaaa
    12661 attctccgcg aacgagagat tcgcagagtt cgacagcaat ttttcctgca tatatgcctg
    12721 tttgttcttc aaggtaggag tacactacat tataaacacc ctttgttgag gtgcttcgtg
    12781 ttcgaccgaa gcctgtgtcc aaaccggcta gcgtttgcaa ttcaagagca atatgttcca
    12841 ccacatggcc catccacgtg ccttcgtcga cgcgtttgaa aaagcctcct gcatggtctt
    12901 cagagcatcg gtgctcatac aaactaggta tgatctgttc cagacgctca cggaacccat
    12961 ggattttatt ggtaggcatt tcctccaact cctccaggtc tagcttcatc acgatcagtt
    13021 tatggcggcg gatgctccaa tagttcgggc ccttcattac ggcggtggaa aggatcttca
    13081 tgtgtttgct ttgcggctaa gttaaaaaaa aggcagatat gatgcttcga attttttaat
    13141 gtagccacgg agacttgttg ccgttagata tcctaaataa ttgtaacgcg atatcgatac
    13201 ttaaattatt gtggatatgt cgatgcattg caatagcctg taacaaatat atttgcccga
    13261 tcattatacc attgcatgat acctaaaggc aggcttatag ccgttggagg cgctgagcac
    13321 cgagatcata ttgaacagga taagctggaa gttttaaggc gaattttaca ggaaatgagg
    13381 ggcccggata ctgtgctgga aatcattcct actgcctccg gcatcccaaa acagattgcc
    13441 agagaatata ctgatgcttt tggcacactg ggttgcaagg ctacgcgtat catgaatatc
    13501 cgcaagcaaa aagatgcgca gaaaaaagaa tttctagacc gtttgcgctc ctgcgatggt
    13561 attatgttca gcggaggaaa tcaaacattg cttagtgaaa catttctggg cacagaattt
    13621 ctggatattt taaagtcacg ctacatcaat gaacccggat ttgtcatcgc aggaaccagc
    13681 gccggagcaa tggctcagag tggcagcatg ataaatgggg gagcccctgc agaagcatta
    13741 atgcgcggaa aagcgcttat gattgaaggt cttggattta ccaataattc cattatagat
    13801 tcccattttg taaaccgagg tcgattcggt cgcttgatgg tagctgttgc tgaacatcct
    13861 cagcttaccg gaattggcat tagtgaagat actgccgtaa tcatcaaaga agaccgctat
    13921 cttgaaatca tcggaacagg actggttgtc attatggacg gaactgaact gcgattaaat
    13981 aatatagcaa ataataaagg tgaaatggta aatctagaac gcatgatctt tcacctcctt
    14041 tcaaaaggca ggcattatga tatgcaggaa agaaaggtga ttacaaatta ataaataccc
    14101 gtgaagtaat ctgcttcgga tttcatccat cctctgtaca tgcccggtgt attaaagggc
    14161 aaacaaatat ttcctttggc atcgatggca attaatccgc cttcagcttc aatgggcttt
    14221 aatttgtcgt gaacgatatg atttgcagct tcctctaatc ccattccttt ataagtcatc
    14281 aggcaatgga gatcatatgc tacaacttgt cgaataaaaa attcgccatg accggtgcaa
    14341 ctcaccgcta cagaagcatt gttggcatag gttcctgagc ctatcaatgg tgaatcgcca
    14401 acgcggccat ataatttgtt ggtcatgcct cctgtagatg ttgctgcagc caaatttcct
    14461 tttgcatcaa tcgcaacagc gcctacagtt ccaaattttt tatcgtcgac atgatctaga
    14521 acagttatct ttttcatcaa tgccttttgc cattgatcat acctcaattg agtgaagaaa
    14581 taatcgtcgt cttcaaacac tatattatgc aatctggcaa attcttcggc tccatcgttg
    14641 agtaataata catgttccga attatccatt acacatctgg ctaaggtgac cggatttttg
    14701 atattgctaa cgcctgcaac agcgccacaa tcgagtgttt cgccattcat aatggatgca
    14761 tccatttcat gctgtccctt gttggtgaat accgatcctt ttccggcatt gaacatgggg
    14821 gcattttcaa gcgctcttac cgcagcttcc acgccatcaa cagcagtgcc acctttggcc
    14881 atggcactgt atcctgcctg taacgccaat tgaagcgaat catggcacgc agcctctatt
    14941 tcaggggtca tgagtctctt tggcatggtg cctgctccac catgtattgc gatgcgaatt
    15001 ggtgcattca tcacataaag gtagaatttt tgatttagca gaacagagtg gtatgcacac
    15061 ctgttgaacc atgacctccg ttaattgttg ttaattaggg aacgcagcga ccatctgaat
    15121 tggttatgtt tgcgtaagtg tgataaagct atcaatatga aaggaactaa aacaatcaca
    15181 gcagcactgc ttagccttgt attaggtatc accggatgtg gtgctaagca ggcaaccagc
    15241 gacaatcaac aaattcaaga taacaagcaa tcaaacgatc tggcaatgcg taaaacttat
    15301 acagatgaag agatccgtaa caacggatgg gaaaaagcaa catttggtgg aggatgcttc
    15361 tggtgcacag aggcgatttt cgaagaagtg aaaggggtta aatcggtagt ttcaggatat
    15421 gcgggcggaa aaatggaaaa acctacatac gaggcagttt gcaccgatac taccggtcat
    15481 gcagagtgtg tgcaagtagt tttcgaccct aaagagtgtg cgtttgcaac aatattggat
    15541 gtacatatga aaacacatga cccgacaaca ataaatcgtc agggaaatga tgtgggaatt
    15601 caatatcgtt cagtagtatt ttatgaaaat gatgcacaaa aaactgctac tgaagattac
    15661 atcaacaaat taaatgcttc cggatactac agcgataaaa ttgttaccga agtggtgcca
    15721 ttaactacat tctggcctgc ggaaacctat catcaggatt attttgccaa aaatcccgac
    15781 caaccgtatt gtgtttatgt ggtagcaaaa aaggttgata aatttgaaca ccttttcccc
    15841 gaattaatag agaaataaat cgtagtttaa tatagtgtaa gtatcatttt tggcgcagac
    15901 tcgttactta aatccagctg caaagaaaat gatgctgcat gcgtactatt gctgaatcca
    15961 ttaatggtaa accactcagt tttaaccggg ccatctgtgc ttgaactatg gttgaaatta
    16021 ctgttgagta agcattctga aaaggttgtg gtaaggctgt caaattgaga tattgcaagg
    16081 gcttcagatg ccaattcgct atatacttca gtatatttac ctcgaatcag tcgaccactt
    16141 gttccattga aaaatgtgtt ttcaaaagtg gaaacctctt catatgtctc ctcatttaat
    16201 accgttgtta ttggaaacat tttccagtcg gcattccatt gattgagcag ataaactaat
    16261 ttcctgcaat caggcatttg tttatcatag aataactgat cgcccgagaa ttgcttgact
    16321 acgtatctga atgtaccggt ttgatatgac tccttacttg ttaacagtat aaaaacagta
    16381 gtatctgcca aggttgtaaa gttgatataa cttccgaaaa catcagattt gatttgctca
    16441 atccatcctt gcacaaaacc ggtcgtatct tcattgatta gcgtaacaaa tggtataaaa
    16501 ctgctgcttt ccatatagac cagcaagcca tctcccgctt ttaaattgat tgaaaaatat
    16561 ttgtaatagt tgccttcctg aagtttgtca tcagcagtgg tgagcgagga ggtatcattt
    16621 gcctccacag gaccaagtgt gatgctttgc gcgttaacaa aactagacgt aatgcaaagg
    16681 atcaaatgca gtatgataat tctcattatg aaggttcttt attcagtttc catccaatcg
    16741 tcgaggtcgc gacgttcttt tttagttggc cttccggtgc ctttttcgcg gaatgcggtg
    16801 ggcataaact ccaaggttgt aagtttcatt ttctcctctt caggggtcac atcttccata
    16861 taatccttca ccagcggagc gccaacacgt ctttccaggg gtgccaaaac cttgtattga
    16921 tactttacga ctccttttgg aatagttagt atcagacccg gaaatacttc aaatgaaggc
    16981 ttgactgagt cgccattgac cttcactttg ccatttttga cagcttcggt tgccaggctg
    17041 cgggttttgt atagtcgaac agcccataac cacttgtcaa ttcgcgtttt tgttacatgc
    17101 tcaggcacga tacttgtaat gttaagccgt aaaaataagc tacattcctg tatcttagcg
    17161 gctataagat tttacatgca aaaacctatt tccgcacgcg agattgtgct gtcacttaaa
    17221 gaccgattcc gccctgagaa ggcagaagcg ggatatgaga ctgtttttca tttggacata
    17281 tcgggcgatc gtggtggaca attcacggtg accattgcca acggaaccat taatattgag
    17341 gacggatttc atggcaatcc caagtgtatt gtgacaacca aagatgaggt atatgaggat
    17401 acagagtggg ggagatccaa tcctcaaatg gccgttatgt ttggtaaaat taaggtgagc
    17461 gacctagggg agatactcga tttcataccc ttgttccacc gttgcgagga gtattatcag
    17521 taaccagtag aataaaaaag cgaaaattga cagctatgcg aaaatttctt tttacagcgc
    17581 tctgtgcctt ttctgcattg actcttgcag cacagaactt caatggactg agcgtttcaa
    17641 ccggaaatat gtatcccgac gggtcaaatt acggtgtagt gcttcaaaat gaacttatcc
    17701 aggttcagca attagatacc actgtgaaat atgatgtggt gtatgaattc aaaaacaaca
    17761 catcgacatt tgctacggtt actgcagcgc ttccggtaaa tgtctatttc aatgaatttg
    17821 ctccgggcaa
//
LOCUS       DAOQOU010000048        17414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1642_length_17414_cov_5.776427, whole genome shotgun sequence.
ACCESSION   DAOQOU010000048 DAOQOU010000000
VERSION     DAOQOU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17414
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_17414_cov_5.776427"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1666
                     /locus_tag="PLM90_06085"
     CDS             224..1666
                     /locus_tag="PLM90_06085"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HMZ88974.1"
                     /translation="MALTLTKSGNPHAATIVYLIRHIDDLNTAALAPFKDLLLSKIND
                     KDNGITLFHSGMGHVVAVLLKSPADNAKVGELLEESRIKGGHICKRLNHEKVTSATVL
                     RASTSVSKEELLAFCEGVALANYQFLKYKSDKKVNSLTSLLIDDAAVDGIELDELNTV
                     IAANHIARDLGNEPVVGLNSVQLGEFAVEAGKRAGFQTEVLNKAQIQSLRMGGLLGVN
                     AGSSVPPTFSILTYKPDNATNIDPFIFVGKGVTYDTGGYSIKTGASMPGMKMDMCGAA
                     AVIGAMYAIATNKLPVYCIGLIPSTDNRINSDALVPDDVITMHDGTTIEVLNTDAEGR
                     LILGDALSYAKKYNPALVIDLATLTGAAAAITGSLGSALMGTDTTYREQLIAGGDLTY
                     ERLWEIPYWKEYADMLKSDVADMKNIGGAIGGSATAGKFLEHFTDYPWLHIDIAGPAI
                     LKDDEAYKQKGASGVGVRLLYSFIKQMAGK"
     gene            complement(1671..2267)
                     /locus_tag="PLM90_06090"
     CDS             complement(1671..2267)
                     /locus_tag="PLM90_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ88975.1"
                     /translation="MKTIICILILALPASLFAQRYPTGKGTYRAGGGGSMSISDKTDY
                     YDYKVTITPRFGYFVTDNLLTGFSMNYTMELDTAFTSAIKFTPQAKYFYKLNPSTFIL
                     ATAEFGLDRSTTYVDPKSIVDHSSVSFGPGVAYYFTRRVGFEINIMTQLYFQPDGSHN
                     NKVYAEGGFVLNVLNKNQKKKNPFKQKGDYEIKEDDDE"
     gene            complement(2306..2911)
                     /gene="udk"
                     /locus_tag="PLM90_06095"
     CDS             complement(2306..2911)
                     /gene="udk"
                     /locus_tag="PLM90_06095"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HMZ88976.1"
                     /translation="MFIIGIAGGSGSGKSTFVRNLVKQLDVDTVSLLMQDAYYRHMPE
                     MPEAERKQMNFDHPDAIEWELLAKHMQTLQSGNIIAQPQYSMLTCLRENETTQVKPNK
                     VLIVEGILVLTQPIVRDLMSLKIFIDADADHRFMRVVKRDMEERGRNIDQVMQRYLKT
                     VRPMHETFIEPSKKHADIIVPIGGDNHVAIDLLTGYIRSRL"
     gene            complement(2921..3982)
                     /locus_tag="PLM90_06100"
     CDS             complement(2921..3982)
                     /locus_tag="PLM90_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HMZ88977.1"
                     /translation="MSSLRAVKYLAGYSIPITAVISMSMHGIAVFITPFYAFVFLAFL
                     EMILPPDHKNLKAMEAELAKKDKVYDILLYFHLPVQFAVLVYFLIQVNEQGLAGYEIA
                     GRIISMGICCGVIGINVGHELGHRVNKTEQRMAQLLLMSSLYMHFFIEHNKGHHKRVS
                     TPEDPSSARMHEPIYFFFVRTIIFSIQSAWQIQCQELREAGRKIISAHNTLLVFFIVE
                     TIFVLAIGFFIGWVVMSYFLIAAGIGILLLESVNYIEHYGLRRQLKSSGLYERVQPWH
                     SWNSDFVLGRVVLYELTRHSDHHYLASKKYQTLNYSEEAPQLPAGYPAMILLALIPPL
                     FFRVMHPRIKALEFQHSTV"
     gene            4107..4724
                     /locus_tag="PLM90_06105"
     CDS             4107..4724
                     /locus_tag="PLM90_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ88978.1"
                     /translation="MIATDNDLQTKIPFALTNVYDFAGFTTLTAQMVAEGTTTGPVKS
                     ESRIDATKLNIQRINRLIKTTKLLPEWDTLFGIENLEFLAITETWCGDGAQILPVVSR
                     IADHIGVPLRIILRDEYPDIIDRYQFRNTRSIPVLIAFNRLTGEELGWWGPKPADAQK
                     IVDDAKINNIPHDDYVISLQNWYNQTKTIPLQQELLGFVQRCLSK"
     gene            4726..5454
                     /locus_tag="PLM90_06110"
     CDS             4726..5454
                     /locus_tag="PLM90_06110"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ88979.1"
                     /translation="MAFFSFTNISTGDNSPQLEKHEEQKLLFLSIFMPVVFIAIMWIV
                     HIFEYVTGMDLGKYGILPKHIEGLPGILFSPFIHGDWQHLISNTAPFLILSFLMIFNY
                     RKVAFKAFVFIYLAGGLLLWLTGRENYHIGASGLIYGMAFFIFFSGVFRKNLQAIALS
                     LFVVFLYGSIVWGMFPIDFKISWEGHAMGAVCGFVAAFYFRNVDRPAPIILEDDPEDD
                     DEMIPHEHEPIEITYTIVDDKPRT"
     gene            5451..6119
                     /gene="mnmD"
                     /locus_tag="PLM90_06115"
     CDS             5451..6119
                     /gene="mnmD"
                     /locus_tag="PLM90_06115"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HMZ88980.1"
                     /translation="MRSTLVITADGSPTIFVPELNEHYHSVHGAVQESMHVFIRAGFD
                     EAAINNASLSILEVGLGTGFNLLLTLNAVIHNQVDVTYHAIEPFPPANEILSAFTSAH
                     LEQLGEGAAYFHALHQVPWEKAAVLHERFTLIKYRDTLSDFTSNSKYHLIYFDAFAPG
                     VQPDMWKQEHFVNLFNLMHSGGILVTYCAKGDVRRAMQSAGFKVERLPGPPGKREMLR
                     ARKI"
     gene            6134..7171
                     /locus_tag="PLM90_06120"
     CDS             6134..7171
                     /locus_tag="PLM90_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ88981.1"
                     /translation="MIDFLIIGGGHAGMHTAFAAMQRKCSFVLVDPFEENATNISAGI
                     INPITGRKYKPQWHIEVLLPFAERIYAAMERQLETSILHHSGIWKMHKSEEALEAWLM
                     AGKTPETMTWVSEDAEQHVWQKHLDHRFGAIQIPKAKRVDTTAMMNAFRKIYAENCMK
                     ESINYAELEVHDDHITWKGEKYRHIVFCEGSRVMQNPWFGHISLWPAKGECLIAHIPH
                     LKSNVILQKKVFIVPFGSDIYWIGASNDFTDATYDPTQAKLDELTEGLRDMLNLPYKI
                     IAHRAGLRPTMRDRTPVVGTHPLHKSLHILNGLGTKGSLLAPYYAHILLDDILHNTGI
                     LAPADVQRTFR"
     gene            7217..9655
                     /locus_tag="PLM90_06125"
     CDS             7217..9655
                     /locus_tag="PLM90_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ88982.1"
                     /translation="MKKPVFSLFSFVAMAICSTLSINAQNTTFNCYLPDQGGSIREHN
                     VDFISLDLDVKFAPEDGLVSGIARYAFTALEPTVDSVFLDGPGIRVQEISLDNNATKF
                     KMDSAGITVYFNPPLIREAVHNMAIIYDANPKKGIYFNGWNVASTDNPNDPTIIRKQI
                     WTQGQGVDNRFWIPSYDGVNDKLLTMMHITFDSTYTVVSNGNLKNVSANADGTKTWRY
                     AMPHPHALYLVMLAIGKYDVMEFTSKNGITTRQYYYPGTKEYAENTYRYTAELMDWMQ
                     QATGLKYPWSTYANVPVQEFLYGAMENTTATIFSDFFYQRPASNPDKQYIEINAHELT
                     HQWFGDYVTAWSGSSHWLQESFATYFSKHFAQHINGDDYYDWKRREEMLTALDADSRD
                     NIPIAHSEAGSQRVYQKGSFVIDMLRYVVGDQAFNVAIQEFLERYPYANVSTQDFQMQ
                     FMRSLGMNMDWFFDEWLYRAGFPVYTVKYDTTENATVVHVSQTQQTNETVHLFNMPIH
                     IQIHYMDGSFDDQLVVIKDAAQDITIQNPEHKQVLFVLFDPNHMVLSKVVFDKSFNEL
                     TYQAFNAPNMIDRYEAISAMTETPIEVKRVTLLALLKKEKFNNIRIEIIRQLSSDNDK
                     ATVAALKNAIYDTDPLVRRSVVVNWNKDNEKLRKDIETLLKDDNYVNIELALRKLCDY
                     YPKQTSEYLQKTAGQFGATNNIRVAWLELQNKDNNNGTMSELIKFTSPQYEFRTRVAA
                     FNAISNRNYCDPELVANLFNALVSTNHRLSSPARNLLMAYKSNPEYAAMIKAYYNSRL
                     WTEWEDTRIGKVKE"
     gene            complement(9659..11809)
                     /locus_tag="PLM90_06130"
     CDS             complement(9659..11809)
                     /locus_tag="PLM90_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ88983.1"
                     /translation="MKAVSFFVLAICLTVKFLYSQAPGILWQNTLQGSKSDLPYSVVN
                     AADGAYFISGISNSESSPDKSENNKGEFDYYDYWVVKMSNSGYVLWENTIGGKRDETN
                     SVVCSTPDGGCIVGGTSQSTATGDKTETSIGGEGDVDFWVLKLDASGNISWQNTIGGT
                     GSDQMRNILATEDGGFLLIGESNSNSGFDKSENGFGGYDFWVVKIDAMGTVVWNKTYG
                     GNLDDLPLSIFQTTDDNYIIAGSSTSGVSGNKTTPNKGNSDFWVIKIDNVGNVLWQRS
                     YGTSKLDHFSDAEQTIDGGIILVGYTNAGITGDKTVATFGMNDYWVIKTNAIGDIQWQ
                     LGAGGNKDDWADAVSISNSGNIYIGGSSMSGVSGNKTVASLGGTFDSWILKLDASGNI
                     VWQKDIQGNDSDYFGDMVVNDENELVAAVYSFSSNSNDKFEPWYGLYDYWIFKLSDTC
                     NAEFCNGMDDDCDGLIDDATMDTIYIAANEGTSCCAGGNVKLQAYHSSGTIQWLRNGN
                     PIPGATGDIYFATKKGNYSCKLNIACELVTSNIIEVKVYKCPVGNIVADGPTTFCDGD
                     SVVLSCTNLPEEDYTYQWYNGMAFIPGALGSTLTVFEPGTYKCLVIRNVGGCGDFSNI
                     IDVHVTCKTSSELSELQIAPNPSNGKFTCSVDPEFGQIQQCKIVDVAGQVVYNVLLNG
                     GADFTIDTSLPTGIYQVLIFNDYGEMQSKPLVIE"
     gene            complement(12109..13095)
                     /locus_tag="PLM90_06135"
     CDS             complement(12109..13095)
                     /locus_tag="PLM90_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88984.1"
                     /translation="MNEEKKSGTRGRVLYLLLILLLLGMNGWLFFNAAQNKDDREKSA
                     RLLEEAKIVNAQLEQQYQTAKKQLDDQKGDFTQKDSLIAVLEAELTSRRNEINALLKT
                     CDFFKGNVKTNEKKIDEVKEEVKTMEVDCSSYKAQLDELNARYLALQEDYEELQFLYE
                     QEVERSEQLQFDKDSILDLAGFILSKDINITGVRKKGNGNDSEGQNAKHTEMLKVCFD
                     VLPNRLSAGKNQTFLLRIISPTGKVLYDQTSGSGEFVNKEKPEDNTYTTSLTVKYDGG
                     EVESHCVYWEQSDEFKPGTYTVKIYHNGYLSSRSSFTLKKPVMEDLMDKVKG"
     assembly_gap    13656..13755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13782..14930)
                     /locus_tag="PLM90_06140"
     CDS             complement(13782..14930)
                     /locus_tag="PLM90_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HMZ88985.1"
                     /translation="MQQRIIVKTFQGFESILASELFALGATDVEVLNRAVQFKGDKAL
                     LYAANIHLRTGLKVMIPLLEGRARNEQQLYDLVKQFDWGDVMRVDDTFSISQAIYSRF
                     FPHGNYAALKIKDAIADHFRDAFGMRPSVDRDNAVIQLHVHIAEDQIQISLDSSGDSL
                     HLRGYRTGTHAAPMSEVQAAGLILLSGWDQKTTFYDPMCGSGTMPIEAALIAANVAPG
                     LIRKHFAFQRWHDYDTALYRQLILDAAAKVKRNKLHVLGSDINGRNVDLAQAHAIAAL
                     PDMKIPFFKKDFQLADPPPEKGVVIMNPPYGERLQHADMIAFYQMIGNVLKQKYAGWT
                     AWVFTGNLEAAKFIGLKPSEKYKLKNGPLDALFLRFDIREGKFWKGQG"
     gene            complement(15001..15537)
                     /locus_tag="PLM90_06145"
     CDS             complement(15001..15537)
                     /locus_tag="PLM90_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88986.1"
                     /translation="MKRILNICLMVAFIGLAAVKSNGQVVMKDFLTEDHVGKVDKAAN
                     MNGGALYYKFEYVSTEGARINYKLHLYKDKGMSTPWMSFDVLMRNLNWTYYVDITMSK
                     DGADKVAALIFKKDLRWGRVKFSPHEGCGRTNPPVWERYTIGEQKGTPEELYAGLLNN
                     YLMQLDKNVDFNCYAGSK"
     gene            complement(15561..16955)
                     /locus_tag="PLM90_06150"
     CDS             complement(15561..16955)
                     /locus_tag="PLM90_06150"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMZ88987.1"
                     /translation="MKLKHLPVVVLTGAVLFATGCGKKSGGGENPLYNDASKPSSEES
                     AAFLLDWWNAMFQFVASERLSPPDAARMYAYISVGLYEAQIAGTPDYLTLEGQLTDLK
                     GLPRPEKGQVYDWQTCVTETMYLVQDGMLARYFPAGVSTLNKLYDKQMESRKAAGVTD
                     EVIERSKAYGEELADAILEWSENDGYVQTRYKQYKAPSREGHPELWEPTDFNQTALEP
                     FWGEHRPFFIKSASQCDIDKAPAYHGVGDTTGELYKQALKVWQVDKTLTEEQRTIAQF
                     WADDPSETSTPPGHWMEILGNFVKSEDMKLDRAAELYALTATAMNDACIAVWYTKYRV
                     NLVRPKTVIHENIEPTWEPYVETPPFAGYTSGHSGFSGAAATIMSGLIGDNLAFTDSS
                     HVNIGLLPRNFASFQAAADEAAYSRMYGGIHFDCDILDGVEQGRCVGNYYLENIKTHP
                     ARKTAAKGEAKKEE"
     gene            17115..>17414
                     /locus_tag="PLM90_06155"
     CDS             17115..>17414
                     /locus_tag="PLM90_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88988.1"
                     /translation="MKASQSVILGLSVLTILSSCGSNKGSKEYLNDWKFTENIDKAKI
                     VQTDGESGKMFDLLSSKTTGINFNNELIETFDLNYYRYGYSYNGAGVGVGDFNNDG"
BASE COUNT         5081 a         3858 c         3397 g         4978 t
ORIGIN      
        1 caggaccctg aattcctcaa agctgccatc aaatggagac ccgacacccc cgatgtgcaa
       61 cgtatattaa tgggctggat ggagcgaaat cgtgacaaca aacaataccg tattgtcgca
      121 ttttgaggca atctgtcatg tcacaaaaca tatattttaa atcctgaatt taggcatttt
      181 agccttacct ttgtggttct gtgtttgaca agaatcccga gcgatggcac ttactcttac
      241 aaaatccggc aacccgcacg cggcaacgat cgtttacctg attcgtcaca tcgacgatct
      301 gaataccgca gcactggctc ctttcaagga tctactactt tcaaagatca atgacaagga
      361 caacggcatt accctgtttc attccggcat ggggcatgta gtggcagttt tattgaaatc
      421 acctgcagat aacgcgaaag taggagaact acttgaggaa tcgaggataa aaggcggtca
      481 catttgcaag cgtttaaatc acgaaaaagt gacatcggcg actgtcttaa gggcttcaac
      541 ctccgtctca aaggaggaac tgttggcctt ttgtgaaggg gttgcgttgg ctaattacca
      601 gtttctgaag tacaaatcag acaaaaaagt aaattccctc acctctttgc ttattgatga
      661 tgccgcagtg gatggcattg agcttgatga actgaatacc gttattgctg caaaccatat
      721 tgcccgtgac cttggtaacg agcctgttgt tggtctgaat tccgttcagc ttggcgaatt
      781 tgctgttgaa gccgggaaac gcgccggttt tcagactgag gtgctgaata aagctcagat
      841 tcaatcgctt cgcatgggcg gattattggg tgtaaatgcc ggtagctccg tgcctcctac
      901 attctctatc cttacatata aacctgataa cgctactaat atcgacccat tcatttttgt
      961 gggcaaaggt gttacctacg ataccggtgg ctatagtatt aaaacaggcg cttctatgcc
     1021 gggcatgaaa atggatatgt gtggcgctgc cgctgtgatc ggagccatgt atgccatcgc
     1081 cacaaataaa ttgcctgttt attgcattgg attgatcccg tcaacagaca atcgcattaa
     1141 ttcagacgca ttagtgcctg acgacgtgat taccatgcac gatggcacta ccattgaagt
     1201 gttaaacaca gatgctgaag gtcgcttgat ccttggtgat gcactcagct atgctaaaaa
     1261 atataatcct gcactcgtaa tcgatcttgc aacacttaca ggtgcagcag ctgctattac
     1321 cggttcgctc ggtagcgcct tgatgggaac ggatactacc tatcgcgaac aattaattgc
     1381 aggtggcgat cttacctatg aaagactctg ggaaatacct tattggaaag aatatgccga
     1441 catgctgaaa agtgatgttg ccgacatgaa aaatattggc ggtgcaatag gtggaagcgc
     1501 tactgccggg aaatttcttg aacattttac cgattatcct tggctgcata tcgacattgc
     1561 aggtccggct attctaaagg atgatgaagc ctataagcaa aaaggtgcaa gtggagttgg
     1621 cgtgaggctg ttatattcct ttattaaaca aatggcaggg aagtgatgca tcattcatca
     1681 tcatcttctt ttatttcata atctcccttc tgtttgaaag gattcttttt cttttgattt
     1741 ttattgagca cattcaatac aaatcctcct tctgcgtaca ccttattatt atgcgaacca
     1801 tcgggttgga aatataattg agtcatgatg ttaatctcaa accctaccct ccttgtgaaa
     1861 taataggcaa cacccggacc aaaactcaca ctgctatggt caacaattga tttcggatca
     1921 acatatgttg ttgatctgtc gagcccgaat tccgcagttg ccaaaataaa ggtgctcgga
     1981 ttgagcttgt aaaaatactt ggcttgtggt gtgaatttaa tggcagaggt aaatgccgta
     2041 tccagttcca tcgtgtaatt catactgaaa ccggtgagca aattatctgt aacaaaatat
     2101 ccaaaacgcg gggtaatggt taccttgtaa tcataataat ctgtcttgtc agaaattgac
     2161 atggaacctc ccccacctgc ccggtaagta cctttccccg tagggtatcg ctgtgcaaac
     2221 agcgaagcag gcaaggctag aattagtatg caaattattg ttttcatgat agcgggaatt
     2281 aaagatacca aattgaataa gacttctaca atcgcgacct gatataacct gtcaatagat
     2341 caatggcaac atggttatcg ccaccaatag gtacaataat atcggcgtgt tttttgcttg
     2401 gttcaataaa ggtctcatgc ataggacgta ccgttttgag ataacgctgc atcacctggt
     2461 ctatattccg tccgcgttct tccatatcgc gctttactac gcgcatgaac ctgtgatcag
     2521 catctgcatc aataaatatt ttgagggaca tcaaatcgcg aacaatgggt tgagtaagca
     2581 ccagaatgcc ttcaacaata agcaccttat ttggcttcac ctgcgtagtt tcattctcgc
     2641 gtaaacaagt aagcatactg tattgcggct gcgcgatgat atttccggat tgcaaagtct
     2701 gcatatgctt tgccaatagt tcccattcta tcgcatcagg atgatcgaag ttcatttgct
     2761 tgcgttctgc ttccggcatt tcaggcatgt gtctgtagta ggcatcctgc atcaatagcg
     2821 aaactgtatc aacatccagt tgtttgacca gattgcgcac aaatgtagat tttccggaac
     2881 cggacccgcc ggcaatacca atgatgaaca tagtaactat ttatactgtt gagtgttgaa
     2941 attccaatgc tttgattcgt ggatgcataa cacgaaaaaa taatggcggt atcaatgcaa
     3001 gcaaaatcat tgccggataa ccggcaggta attgaggggc ctcctcacta tagtttaaag
     3061 tctgatattt ttttgatgca agataatggt gatccgaatg gcgagtcaat tcatacaaca
     3121 ctacccttcc taacacaaaa tctgagttcc atgaatgcca tggttgtaca cgctcgtata
     3181 aacccgaaga ttttaattga cgacgcagcc cataatgctc aatataattc actgattcta
     3241 ataataatat accaatgcct gcagctatca gaaaatagct catcaccacc caacctatga
     3301 aaaatccgat ggcaagtaca aatatcgttt caacaataaa aaaaactaat aaggtgttgt
     3361 gtgccgaaat aattttcctt cccgcctccc gaagttcctg acactgaatt tgccaggcac
     3421 tttgaattga aaaaataata gtgcgaacga aaaagaagta aatcggctca tgcatgcgcg
     3481 cactagaagg atcttccgga gttgatacgc gcttgtggtg acctttattg tgctcaataa
     3541 aaaagtgcat atatagcgac gacatcaata acaattgtgc catgcgctgc tcggttttat
     3601 ttactcgatg gcctaattcg tgcccaacat tgatccctat caccccacaa caaatgccca
     3661 tactaatgat gcgccctgca atttcatatc ctgccaaacc ttgctcattc acttggatta
     3721 aaaagtaaac aagcactgca aattgaaccg gtaaatgaaa atacaacagg atgtcataca
     3781 ccttatcctt ctttgccaat tcagcctcca tagcctttag atttttgtga tctggaggca
     3841 atatcatttc caaaaaagcc aggaaaacaa aagcataaaa tggggtaata aatacagcaa
     3901 tgccatgcat gctcatcgaa atcactgctg tgatcgggat gctgtaaccg gcaagatatt
     3961 ttactgcacg caaagatgac atcgctcaaa attatcaaaa tctatgtgaa taggcttatg
     4021 tgaccaaagg cactctgtga cctacatcac catttcgccc gtgaacgctt tgcacctttg
     4081 tgatgttcta ctaaccggaa cgcattatga ttgccacaga taacgaccta caaacaaaga
     4141 tccctttcgc gctgacaaat gtgtatgact ttgccggctt tacgaccctg actgcacaaa
     4201 tggttgctga aggcactaca acgggtcctg tgaagagcga atcacgcatt gatgccacta
     4261 aactcaatat ccaacgcatt aaccgcctga taaaaaccac gaagctgctt cctgaatggg
     4321 acaccctttt tggcattgaa aatctcgagt tcctggctat tacggaaacc tggtgtggcg
     4381 atggcgctca aatcctgcct gttgtaagtc gcattgccga ccatatcggt gtgccccttc
     4441 gcatcatcct tcgcgatgaa tatccggaca tcatcgaccg gtaccaattc cggaatacgc
     4501 gttccattcc cgttctgata gcctttaaca ggcttaccgg cgaagaatta ggctggtggg
     4561 gaccaaagcc tgccgatgct cagaaaattg ttgatgacgc caaaatcaac aatataccac
     4621 atgacgatta cgtcatatct ttacagaact ggtataatca aactaaaaca attcctttgc
     4681 aacaggaact gctgggcttt gttcagcgtt gtctgtcaaa gtaatatggc ttttttctca
     4741 tttacgaata tatctaccgg cgacaatagt ccgcaactgg aaaagcatga agaacagaaa
     4801 ttgctattcc tcagcatttt tatgcctgtt gtttttatag caatcatgtg gatagttcac
     4861 atctttgagt atgtgacagg aatggacctc ggcaagtatg gaattttgcc caaacatatt
     4921 gaaggcttgc ccggtatatt attttcacca ttcatccatg gcgattggca acatctcatc
     4981 agcaatactg caccttttct gatcctgtca tttctgatga tcttcaatta tcgaaaggta
     5041 gcatttaaag catttgtatt catttatctt gccggtggat tattgctgtg gcttacaggt
     5101 cgagagaatt atcatattgg tgccagcggt ttaatttatg gtatggcatt tttcattttc
     5161 ttcagtggcg tatttcgtaa aaatttgcaa gcaatagcgc tttccttatt tgtggtgttt
     5221 ttatatggca gtattgtttg gggaatgttt cctatcgatt ttaaaatatc gtgggaaggc
     5281 catgcgatgg gtgctgtatg cggatttgta gcagccttct attttagaaa tgtcgacaga
     5341 ccggcaccga ttattttaga agacgatcct gaagatgatg atgaaatgat acctcatgaa
     5401 catgagccca ttgaaattac atataccata gtagacgata aaccccgaac atgaggtcta
     5461 ctcttgtcat tacggccgat ggatcaccaa caatttttgt tcctgaatta aatgaacatt
     5521 atcattcagt gcacggcgct gttcaggaat ctatgcatgt atttattcgc gccggatttg
     5581 atgaagctgc aataaataat gcatcgcttt ccatactcga agtaggccta ggtacaggat
     5641 ttaatttgct gctgacatta aatgctgtca tacataatca ggttgatgta acttatcacg
     5701 ccatagagcc atttcctccc gccaatgaaa tactttcagc atttacctct gcacatttag
     5761 aacaactcgg tgaaggtgca gcgtattttc atgcattaca ccaagtccct tgggagaaag
     5821 cggcagtatt acacgaacgg tttaccttga taaaatatcg tgacaccctg tcggatttta
     5881 cctccaatag caaatatcat ctcatatatt tcgacgcctt tgctcccgga gtgcagcccg
     5941 atatgtggaa gcaagaacat ttcgtcaacc tgttcaacct catgcattcc ggcggtattt
     6001 tagtaaccta ttgtgctaaa ggagatgttc gcagagctat gcaatcggca ggattcaaag
     6061 tagaacggct acccggacct ccgggaaaac gcgaaatgct gcgtgcgaga aaaatctgat
     6121 ttatttttga cctgtgattg attttctcat cataggtggc ggtcatgccg gtatgcatac
     6181 agcgtttgca gcaatgcaac gtaaatgttc atttgtgctt gttgaccctt ttgaagaaaa
     6241 cgctacaaat atttcagccg gtataataaa tcctattacc ggaagaaaat ataaaccgca
     6301 atggcatata gaagtattat tgccatttgc agaacgcata tatgctgcca tggagaggca
     6361 attggaaaca agtattttgc atcattccgg catatggaaa atgcataaaa gtgaagaagc
     6421 ccttgaagcc tggctgatgg cagggaaaac acctgaaaca atgacttggg tgagtgaaga
     6481 tgctgagcaa catgtatggc agaaacacct ggatcaccga tttggggcca tacaaattcc
     6541 gaaagcaaaa cgcgtagata ctactgcaat gatgaatgct tttcggaaga tatatgctga
     6601 aaattgtatg aaagaatcaa ttaactatgc tgaattggaa gtccatgatg accatatcac
     6661 atggaaaggt gaaaaatata ggcatattgt attttgtgaa ggctcccgtg taatgcaaaa
     6721 tccatggttt ggacatatca gtttatggcc tgctaagggt gaatgcctga tagcacatat
     6781 tccacatttg aaatcgaatg tgatactcca aaaaaaggtg tttattgtac cattcggctc
     6841 agatatctat tggataggtg ccagcaatga ttttacggat gccacatatg atcctactca
     6901 agccaaactg gatgaattga ctgaagggtt acgcgatatg ctcaatcttc cttacaaaat
     6961 cattgcacac agagccgggt tgcggccaac catgcgcgac agaacccctg ttgttggcac
     7021 acatccactt cacaaaagcc tgcatattct caatggcctg ggaacaaaag gaagccttct
     7081 ggcaccttat tatgcacata ttctgttgga tgatattctg cataacacag gaattctcgc
     7141 accagccgat gtgcagcgca ctttccgtta acaaacctta tttgtcgggg cgggggtgaa
     7201 gaattatttt tacctgatga agaagccggt attttctttg ttttcattcg ttgccatggc
     7261 aatatgcagc accttaagca taaacgctca gaatactaca tttaattgct acctacctga
     7321 ccaaggtgga agcattcgcg aacacaatgt agattttatt tcccttgacc tagatgtgaa
     7381 gtttgcaccg gaagacggat tggtaagcgg cattgctcga tacgctttca ctgcgctcga
     7441 gccaactgtt gattcagtat ttcttgatgg acctggcatc agagtgcagg aaatttcgct
     7501 tgacaacaat gctactaaat tcaaaatgga tagcgccggt atcactgttt acttcaaccc
     7561 accactaata cgagaagcag tacataatat ggccatcatc tacgatgcga accctaaaaa
     7621 aggtatctat ttcaatggtt ggaatgttgc ttcaactgat aatccgaatg atcctactat
     7681 cattagaaaa caaatttgga cacaaggtca aggcgtagat aacagatttt ggattccttc
     7741 ttacgacgga gtgaatgaca agctcctcac catgatgcat ataacgtttg actccacata
     7801 tacagttgta tcaaatggca atctaaaaaa tgtaagcgcg aatgctgatg gcactaaaac
     7861 atggcgatat gctatgccac atcctcatgc actatatctt gtcatgttgg ctataggcaa
     7921 atatgatgtg atggaattca cttctaaaaa tggtatcact acacgccaat attattatcc
     7981 cggaacgaaa gaatatgctg aaaacactta tcggtatact gccgaattaa tggattggat
     8041 gcaacaggcc accggattaa aatatccatg gtctacttat gcaaatgttc ccgtacagga
     8101 atttttatat ggcgccatgg aaaataccac cgctactatt ttcagcgatt tcttctatca
     8161 acgccctgct agcaatcctg ataaacaata tatcgaaatc aatgcacacg aattaacaca
     8221 tcaatggttt ggcgattacg taactgcatg gagcggatcc agtcactggt tgcaggagag
     8281 tttcgcaaca tatttctcga agcattttgc ccaacatatt aacggcgatg attattatga
     8341 ctggaaaaga cgcgaagaga tgctaactgc tttagatgca gacagcagag ataatattcc
     8401 aattgcgcat tctgaagcag gctcacagcg cgtatatcaa aaaggcagtt ttgtaattga
     8461 tatgctgaga tatgttgttg gtgaccaggc atttaatgtg gccattcagg aattcctaga
     8521 acgctatcct tatgcgaatg taagtacaca agattttcaa atgcaattta tgcgctcatt
     8581 gggaatgaat atggattggt tttttgatga atggttatat agagccggat tcccggttta
     8641 taccgtgaaa tatgatacaa ccgaaaatgc taccgtagtg catgtttcac aaacccagca
     8701 aacaaatgaa acggtgcact tgttcaatat gccgattcat attcagattc actatatgga
     8761 cggaagtttt gatgatcagt tggtagtaat caaagatgct gcacaggata tcaccattca
     8821 gaatccggaa cacaagcaag tgctttttgt actttttgat ccgaatcata tggtattgtc
     8881 gaaagtagtt tttgataaat ccttcaacga gctcacctat caggctttta atgcgccaaa
     8941 tatgatcgac agatatgagg cgatatccgc aatgaccgaa acgccgattg aagtaaagag
     9001 agtaacattg ctggcactat tgaaaaaaga gaaatttaat aatatcagga tagaaatcat
     9061 tcgacaatta agcagcgaca atgataaggc aactgtagca gccttaaaaa atgcaattta
     9121 tgatacagac cctcttgtaa gaagatcggt ggtagtaaat tggaacaagg ataatgaaaa
     9181 gctgagaaag gatatcgaaa cacttttaaa ggacgataat tatgtgaata ttgaattagc
     9241 acttcgtaag ctatgtgatt attacccaaa acaaacttca gaatatttac aaaaaacagc
     9301 cggacaattt ggagcaacaa ataacattcg cgtggcctgg ctggaattgc aaaataagga
     9361 taacaataat ggcacgatga gcgaattgat taaattcacg agccctcaat atgaattcag
     9421 aacccgtgtg gcagcattca atgccatcag caatcgcaat tattgcgatc ctgagttagt
     9481 tgcgaattta ttcaatgcac ttgtgagcac caatcaccga ttatcatctc cggcaagaaa
     9541 cctgctgatg gcatacaaga gcaatcctga atatgccgct atgataaaag catattataa
     9601 ttccagactc tggactgaat gggaagacac caggatcgga aaggtgaagg aataaactct
     9661 actcaatcac gagtggttta gactgcattt cgccatagtc attgaatatc aatacctggt
     9721 atattccggt tggaagactc gtgtcaattg taaaatcggc tcccccattt aataggacgt
     9781 tgtatacaac ttgacctgca acatcaacta tcttgcactg ttgaatttgt ccaaattcag
     9841 gatctaccga gcaggtaaat tttccatttg acggattggg agcaatttgc aattctgaaa
     9901 gttcggaaga agttttacaa gtaacatgca catctataat attagaaaaa tcgccacacc
     9961 ctcctacatt acgaattacg agacatttat acgttcccgg ctcgaaaact gtcaatgtgc
    10021 tgcctaaagc acccggaata aatgccattc cattatacca ttgataagta taatcttctt
    10081 caggcaagtt cgtacatgaa agaactacac tatcaccatc acaaaaagtt gtcggaccat
    10141 cggcaacaat gtttcctaca gggcatttat aaactttgac ctcaataatg ttagatgtta
    10201 ccaattcgca tgcaatattt aatttacagg aataatttcc cttttttgtg gcaaaataga
    10261 tatcgccggt tgctcccggg ataggattac cgtttcttag ccactgaata gtaccggaac
    10321 tatgatatgc ttgcaattta acattaccgc ctgcgcaaca ggaagtgcct tcattagctg
    10381 caatataaat agtgtccatt gttgcatcat ctatcaaccc atcacaatca tcgtccatac
    10441 cattgcaaaa ttcggcgtta caggtatctg ataatttgaa tatccagtag tcgtaaagtc
    10501 cataccaagg ctcaaatttg tcattagaat ttgacgagaa tgaatatacg gcggcaacca
    10561 gctcattttc atcatttacc accatatctc caaaatagtc gctgtcattt ccttggatgt
    10621 ccttctgcca aactatattt cctgatgcat ctagttttaa tatccaacta tcaaatgttc
    10681 ctccaagcga tgcaacagtt ttatttccgg atacaccgga cattgagctg ccaccaatat
    10741 aaatgttacc tgaattacta atagaaacgg catctgccca gtcatccttg ttaccacctg
    10801 cgccgagctg ccattgaata tcgcctattg cattcgtctt aattacccaa taatcgttca
    10861 ttccaaatgt cgccaccgtt ttatctccgg ttatacctgc gtttgtatat ccgaccaata
    10921 tgatgcctcc atcaatcgtt tgttccgcat ctgaaaaatg gtccaatttt gaagtgccat
    10981 aagaacgttg ccacaacaca ttgccaacat tatcaatctt aatcacccaa aaatcactgt
    11041 ttcctttatt gggcgtggtt ttatttccgg atacaccgga tgtggaggat ccggcaatga
    11101 tataattgtc atcggtcgtt tgaaaaatac ttaatggtag atcatccaaa ttgccgccat
    11161 aggttttatt ccatactact gttcccatag catcaatttt gacaacccaa aaatcatatc
    11221 caccgaaacc attctcagac ttgtcaaatc ctgaattaga atttgattca ccaatcaaaa
    11281 gaaatccacc atcttcagta gctaatatat tacgcatctg atcagatcct gttcctccta
    11341 ttgtattttg ccaggaaata ttaccggatg cgtcaagttt taacacccaa aaatctacat
    11401 caccttctcc ccctatactc gtctctgttt tatcaccggt tgctgtagat tgcgaagtac
    11461 ctccaacaat acaaccgcca tcaggtgtgc tgcaaacaac tgaatttgtt tcatcgcgtt
    11521 tacctccaat tgtgttctcc cataacacat aaccggaatt gctcattttc acaacccaat
    11581 aatcgtaata atcaaattca cccttattat tttcactttt atcaggtgaa gattccgaat
    11641 tcgaaatacc ggatatgaaa tacgccccat cggcagcatt gacaacagaa tatggcaaat
    11701 ctgatttact cccctgaagc gtgttttgcc ataatatacc cggggcctga ctatataaaa
    11761 atttaactgt caggcaaatg gctaaaacaa aaaaggaaac tgctttcata aacattacaa
    11821 tttattactt ttaataaaat tgattcgtca aagctacatg gacgattgca atagcaccaa
    11881 agaagaattc acgatatttg ggtataattt caaattattt gctaaatccg ggatattaac
    11941 aattcaaaaa ttaaccagaa aaaagcaata tattttctaa tgcagctgcc gacaattaat
    12001 ttctgataaa attaaatatt cacgcataac aaaaggctca tcaaaatcat cctataaaaa
    12061 aaccccggtg ttacccgagg tttctccttt catacttata gcaaaaagct accctttaac
    12121 tttatccatc aagtcttcca ttaccggttt tttcaaggta aatgaactgc gacttgaaag
    12181 gtatccattg tgataaattt ttacggtata agttcccggt ttaaattcat ctgattgttc
    12241 ccaatataca cagtgactct caacttcacc tccgtcatat tttactgtaa ggctggtggt
    12301 ataagtattg tcttcaggtt tttccttatt cacaaactct cctgagccac ttgtctgatc
    12361 gtataacact ttacctgtcg ggcttatgat acgcagtaaa aatgtttgat tcttacccgc
    12421 cgataatcta ttcggcaaca catcaaaaca tactttcaac atttcagtat gcttcgcatt
    12481 ttgtccttct gaatcattgc catttccttt tttgcgaaca ccggtaatat tgatgtcttt
    12541 cgacaaaatg aaaccggcaa gatcaagaat cgaatctttg tcaaattgca actgttcact
    12601 gcgctcaact tcctgctcat acaagaattg cagttcttca taatcttcct gcaatgccag
    12661 ataacgcgcg tttagttcgt cgagctgagc cttgtaagaa ctacaatcta cttccatcgt
    12721 cttcacttcc tccttcactt catcaatttt cttctcattg gttttcacat tgcctttgaa
    12781 gaaatcacaa gtcttcaaaa gggcattgat ctcattcctt cgagaagtca attcagcctc
    12841 caataccgca ataagactgt ctttctgggt gaaatcacct ttctgatcgt ctaactgctt
    12901 ctttgcagtc tgatactgtt gctccagttg tgcgtttaca atcttggctt cttccaacaa
    12961 acgggcactc ttctcgcggt cgtctttgtt ttgagccgca ttgaagaaca accatccgtt
    13021 catccccaga agaagtagga tgagtaacaa gtacagcacc cttccacggg taccgctttt
    13081 cttctcttca ttcatacaag acaggcagga tttttcgcca ttgcggttga accggaccgc
    13141 agatagcccc cttgccttat tcaaaattga atgaaaccac tatctcaacg aaataatcta
    13201 atgcacacag tactttggta cagaggcatt tatgtggtac aactacaata tccacacaca
    13261 tgtacctacc cctttcgaaa tttaaacccc ttgtcagtac agggtttcag gtacaacaga
    13321 cgccttgcgg tacatgggat tcaggagttg tgtacacaat tttcggcata tgtggataac
    13381 ctgttgaaaa aggaggagag aggagaaagg agagtgggtg agtgggtgag ttttattaaa
    13441 ggcaaaaaaa atcccgaccg caggtcgggg aaataactca ccacccgcct tctcggcgag
    13501 gtaaactcac ccactgaccc actcacccac tcacgattca cgattcacga ttcacgagtc
    13561 acgattcggt agtgggtatt gagtagtgag acaaatgcac ccactcacca ctcaccactc
    13621 acccactcac ccctgcccct tccagaactt accttnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnccact caccactcac cactcaccca ctcacccctg ccccttccag
    13801 aacttacctt ctctgatatc gaatctcaaa aacagggcat ccaatggtcc gtttttgagt
    13861 ttgtattttt cggatggttt gaggccgata aactttgctg cttccagatt accggtgaaa
    13921 acccaggctg tccaaccggc atatttttgt ttgagcacgt tgccaatcat ctgataaaat
    13981 gctatcatat cggcgtgctg taatcgctca ccatagggcg gattcataat cacaacaccc
    14041 ttttctggag gcggatctgc aagctgaaag tcttttttga agaatggtat cttcatgtca
    14101 ggaagcgcgg caatagcatg cgcctgtgca agatccacgt tcctgccatt gatatctgat
    14161 cctaacacat gtaatttatt tcgcttcacc ttagctgcag catccaaaat aagctgtcgg
    14221 tagagagcgg tgtcatagtc gtgccaacgc tgaaatgcaa agtgttttct gatgagtccg
    14281 ggagcaacat tcgcagcaat aagtgctgct tcaataggca tagtgccgct gccacacata
    14341 gggtcataaa atgtagtttt ctgatcccaa ccactcagca agatcaagcc ggctgcctgc
    14401 acttcactca tcggtgcagc atgagttccg gtgcggtatc ctctcaagtg gagactgtcg
    14461 cccgagctat caagtgaaat ttgaatctgg tcttcagcaa tatgcacgtg taattgaatt
    14521 actgcattat ccctgtccac acttggtcgc attccgaaag catcacggaa atggtctgca
    14581 atggcatctt tgattttcaa tgcagcataa tttccatgcg gaaaaaacct cgaataaatg
    14641 gcttgagaaa tactaaaagt atcatcaaca cgcattacat caccccaatc gaattgtttc
    14701 accaggtcgt acaattgttg ttcattgcgg gctctgcctt ctaaaagcgg tatcatcact
    14761 tttaatcccg tacgcaggtg aatatttgcg gcatataaca gggctttatc acccttaaat
    14821 tgcacagccc tgtttaacac ctcaacatcc gtagctccaa gcgcaaacaa ttctgacgcc
    14881 agtatgcttt caaatccctg aaatgttttt actataatgc gttgttgcat atgtgggtgt
    14941 aaaaataaga aaggcgttgt tgtgcacaac gcctttcatg aatgtattag atacggcaga
    15001 ttatttactt cctgcgtagc aattaaaatc aacgtttttg tcgagctgca tcaggtaatt
    15061 attcaaaaga ccggcatata gctcttcagg tgttcctttt tgttcgccga tggtgtaacg
    15121 ctcccataca ggtgggtttg ttcggccaca accttcatgt ggcgagaatt tcacacgacc
    15181 ccaacgaagg tcttttttga aaatcaatgc ggctaccttg tctgcaccgt ccttagacat
    15241 cgtaatatcg acataataag tccagttaag attgcgcatc aacacatcaa agctcatcca
    15301 tggcgttgac atgcctttgt ctttatacaa atgcaatttg taattgatac gtgctccttc
    15361 agtacttaca tactcgaatt tgtaatatag agcaccaccg ttcatattcg ctgccttatc
    15421 aactttacct acatggtcct ctgtaagaaa gtctttcatc accacctgtc cgttagactt
    15481 aacagcggcg aggcctataa aggcaaccat caggcagata tttaatatcc ttttcattgt
    15541 ttctgtggtt taatggttta ttattcttct tttttggctt caccttttgc agcagttttg
    15601 cgtgcagggt gtgttttaat attttcgaga tagtagttac ctacacaacg gccttgctca
    15661 acaccatcaa gaatgtcgca gtcgaagtga ataccaccat acatacgact gtaagcagct
    15721 tcatcagcag cagcctggaa tgatgcaaaa ttgcgtggta ataaaccgat gtttacatgt
    15781 gaactatctg taaatgcaag gttgtctcca atcaatccgg acatgatggt agccgcagca
    15841 ccactgaatc ctgagtgacc tgaagtatat cctgcaaatg gaggtgtctc aacatatggt
    15901 tcccaagttg gttcaatatt ttcatgaata accgtttttg gtcgaaccag atttacacgg
    15961 tatttagtat accataccgc aatacaggca tcgttcatcg ctgtagctgt taacgcatac
    16021 aactcagctg cacggtcgag tttcatgtcc tcagacttaa cgaaattacc aaggatttcc
    16081 atccagtgtc cgggaggcgt agacgtttca cttggatcat cagcccagaa ttgagcgatg
    16141 gtgcgttgtt cttcagtcaa tgttttatcc acctgccata ctttcagtgc ttgtttgtaa
    16201 agctctcctg tagtgtcacc aactccgtgg taagcaggtg ccttatcgat atcacactgt
    16261 gaagcagatt ttataaagaa aggacgatgt tcaccccaga atggttccaa cgctgtttgg
    16321 ttgaaatctg ttggttccca caattccgga tgtccttcgc gtgaaggcgc tttatattgc
    16381 ttgtagcgag tttgcacgta tccgtcgttt tcgctccatt caagaatcgc gtctgcaagt
    16441 tcttcaccat aagctttaga acgctcaatt acttcatcgg taacaccggc agctttgcgg
    16501 ctttccattt gcttatcgta taatttattc aacgtactta cccctgcagg gaagtaacgt
    16561 gccaacatac catcctgaac caaatacatg gtttcggtta cgcatgtttg ccagtcatat
    16621 acttgacctt tttcaggtct tggaagacct ttgagatctg taagctgacc ttcaagagtt
    16681 aaataatcgg gcgtaccggc tatctgtgct tcatataggc ctacagaaat gtatgcatac
    16741 atacgcgcag catctggcgg agacaaacgt tcgctggcta caaattggaa catcgcattc
    16801 caccaatcaa gaaggaaagc tgcgctttct tcactggaag gtttggaagc atcgttgtaa
    16861 agtgggttct ctcctccacc ggattttttt ccgcatcctg tggcaaaaag cacggcacct
    16921 gtaagtacaa cgacaggtaa gtgttttaat ttcatgactt ctaagatttt agtttagcag
    16981 ttgagtgatt gaaataatcg ctgcaaaatt aagcaaattt agccatcggc accccattaa
    17041 caaaacattt acagaggtcg tacttacccc ccttttaagc aggttacctt tgcagttcaa
    17101 accgtaaaca aggcatgaaa gcttcacaat ccgtaatcct tggcctaagt gtgcttacca
    17161 ttttaagcag ctgtggttct aacaagggca gtaaagagta tctcaacgac tggaaattca
    17221 ctgagaacat cgacaaagcc aaaatcgtac aaaccgatgg cgagtcagga aagatgtttg
    17281 atctgctttc cagcaaaacg acagggatca acttcaataa tgaacttatt gaaacctttg
    17341 acctcaacta ttaccgctat ggctattcct ataatggcgc cggtgtaggt gtgggcgatt
    17401 tcaataatga tggt
//
LOCUS       DAOQOU010000049        17064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1684_length_17064_cov_6.050797, whole genome shotgun sequence.
ACCESSION   DAOQOU010000049 DAOQOU010000000
VERSION     DAOQOU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17064
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1684_length_17064_cov_6.050797"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PLM90_06160"
     CDS             complement(<1..1214)
                     /locus_tag="PLM90_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ88989.1"
                     /translation="MKHKLLLIASICMLRALPSGAQTPAWAKEAIWYQIFPDRFRNGD
                     PSNDPTLADIKGCWPHNDTAAWEIMPWTSDWYAMQPWELANGQDFNYNVQRRRYGGDL
                     QGIIDRLGYIDSLGINAIYLNPVFWAPSLHKYDAVMYHHVDPNFGPDPEGDKKLIASE
                     DPINPATWHWTAADKLALQLINSCHKRGIKIIFDGVFNHMGINSFAFQDVVKNQQDSK
                     YKDWFTIYSWDDSLAGTTFKYQGWFGVPDLPEIKEDSMGIVAGPKQYIFDCTKRWMAP
                     DGNAANGIDGWRLDVAFCVDHDFWKSWRLWVKSINPEAYLTAEVIDKKEVVAPYLQGD
                     EFDAVMNYGFGVIASEFFINKQSRISVTTFDSLLADLRQAYRPDFTYVMQNLYDSHDT
                     QRFTSFLHNPDIA"
     gene            1244..3526
                     /locus_tag="PLM90_06165"
     CDS             1244..3526
                     /locus_tag="PLM90_06165"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ88990.1"
                     /translation="MKSSLLIFYLCLLSTFVAKAQMTPPYSILYDDTQVNKIYITMDI
                     DSLSEMYDELENEHEYAVQFVFDRGTQTDTLQDVGFRLRGNTSLSSAKKSFKVSFNTY
                     NPGRTFETAKKLNLIGNHNDPTMVREKTYFDIYNRLGLPMRRVGFAEVYINDQYFGFY
                     TVVEEYDDIWCRDRYGDDAGALFKCLYGSTFTYNGENTGAYNTYEQQSNEQVNMKGEL
                     AHMFDVLNNTPIADLPCAFEPLFDVDQFLKIYALDISTGHWDNYGANQNNYYLYHDLK
                     SGQYKFLSYDCDNVLGIDWFGIDWGTRNVYDWNFDGRPLVERLLQVDVYRERFSYYLN
                     LIATYGMDTDYWYPRLDDIRDLIAPSALADIYRTMDYGYSYGDFLDGFDTDDIDGHCP
                     YGIKNFFDARINSTLDQVELSDIAPIIHFPQQDHMLLEHGDYLNITVFIEEDVSVSAV
                     QLLYSNNETSYSTADLYDDGAHGDGIAGDNIYGNTVIVPMDGTTFYYHIIASDNTGNE
                     SVYPVCENIARTIGYTTPTLHINEVMPDNVSTVPDGAGEFPDFIELYNYGLYAIDISN
                     LFITDELDRPTKWRLPDMMLPFDSYVLIFADNETEQGLNHATFSIDADKDDLFIYGDV
                     TTDYYLIDSTSFKNIPPDVSWGRLPNGTGPFTNLPVASPGAYNTVDTASVPDLFEVIL
                     TNNPGTHTSEVIITNDIAQTITLHIFNMAGQEVFKEAPATLPAGTYSYSIPANRLGSG
                     AYLIRIGHGDKVTMITYSVL"
     gene            3583..4302
                     /locus_tag="PLM90_06170"
     CDS             3583..4302
                     /locus_tag="PLM90_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMZ88991.1"
                     /translation="MGRAFEYRKERKFKRWGAMATAFTRVGREIAIAVKNGGPNPEAN
                     PKLRIAIQNAKAVQMPKDRVEAAIKRASSKDEKDFEEVVYEGIGPGGVAIMVECATDN
                     TTRTVANVRVLFSKGGGALGTSGSVGYLFERKGIFKVESGKIKLDVVELDLIDGGAED
                     IYEEDGTVYIETSFNDFGNMQKKLDELNIEISGAELLRIPMNKNAVPPDAEKLVHNLI
                     EKLEEDEDVVNVFHNMVETME"
     gene            complement(4632..4826)
                     /locus_tag="PLM90_06175"
     CDS             complement(4632..4826)
                     /locus_tag="PLM90_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HMZ88992.1"
                     /translation="METGKIKFFNATKGYGFITKDGTNEEIFVHQTGLVDKVKQNDLV
                     TFDIIDGRKGKNAVNVKLAQ"
     gene            complement(4881..7289)
                     /locus_tag="PLM90_06180"
     CDS             complement(4881..7289)
                     /locus_tag="PLM90_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMZ88993.1"
                     /translation="MSESLSTTQLTFESFRANVINDFVTACLSREISLLGRKEVLTGK
                     AKFGIFGDGKEVAQVAMARVFKEGDWRSGYYRDQTFMFASGMATPMQFFAQLYAIPDI
                     SMDPFSGGRQMNAHFATRTIDANGNWNDLVNVKNSSSDTSPTASQMARAVGLGLASKL
                     YRSNPGVHTQGFSNNGNEVVFTTIGDASTSEGLFWESINAAGVLKIPMAVFVWDDGYG
                     ISVPQKYQTTKQNISEILSGFQLDEDGHGVRIYEVKGWDYPSMVQTFEEGIDLCRREH
                     IPVLFHVTEITQPQGHSTSGSHERYKSKERLEWEAEWDCVRQMRKWMIAASVATEEEL
                     NDIVAKAKDEARQHQKAAWNAHLMSIKTDMDVVFSLLENLRNSSNASGQIAAIITELK
                     DTMDAVRKDIMRAARLALHATAGEQTIERAALQAWAEDYAALNDQRYNGNLFSNSAHA
                     IQHIPVIPAQYPEEPTMVNGFELLNKCFDMHFSTNPRIYAFGEDVGKIGDVNQGFAGL
                     QEKHGEHRIFDVGIREATIIGQGIGMALRGMRPIAEIQYLDYLIYGLQPISDDLATLQ
                     YRTAGGQKAPLIIRTRGHRLEGIWHTGSPMGMILNTCKGVHLCVPRNMTQAAGMYNTL
                     LQSDEPAIVVECLNGYRLKEALPLNINTFTVPLGVPEILRNGTDITVVSYGSTLRVVE
                     EACDRLAALGMSIELIDAQTLMPFDVQGIIAASVRRTNRVLFVDEDVPGGASAYMMQQ
                     VFADETLFRSLDAFPKCLSAKANRSAYASDGDYFCKPSEEDVFEAIYAMVSEVDPGQY
                     PAIS"
     gene            7489..7977
                     /locus_tag="PLM90_06185"
     CDS             7489..7977
                     /locus_tag="PLM90_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ88994.1"
                     /translation="MKKFTYTLALTMLIATASFAQTSTTPPAGSSEATTPVVNPNAGE
                     FQWSEETYDFGTIAQGVPVKHKFEFTNTGKEPIIVSNVVKTCGCTVTDWTKEPVLPGQ
                     KGQVIAEFNAAKEGPFTKAITVQSDAKTPSVKLYFKGTVQKSEQAGSVPENNNIFNQG
                     GN"
     gene            8005..8460
                     /locus_tag="PLM90_06190"
     CDS             8005..8460
                     /locus_tag="PLM90_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ88995.1"
                     /translation="MKKVLSIVAFLAVTGAMTAQTATGTGTTAAVSENAAEFQFVTEV
                     FDFGQIPQGVPAEARFEFTNTGKEPLIITDVQKTCGCTKTDWSKEPIAPGAKGWIIAE
                     YNAANEGSFTKAITVFSNTKTPNMKLTFKGTVVKDESGSVPQQKSIFDN"
     gene            8605..9831
                     /locus_tag="PLM90_06195"
     CDS             8605..9831
                     /locus_tag="PLM90_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ88996.1"
                     /translation="MPHISQRGADMPASPIRKLVPFAEKAKLRGVKIYHLNIGQPDIV
                     TPPQMLDAVKRTDLKVIEYSHSAGFESYRRKLVTYYESRGIHINHHQIMITTGGSEAI
                     LFGMMSCLDAGDEVIIPEPLYANYNGFSTAGNIVIKPITSHIENGFALPPIAEFEKLI
                     TPKTKAILICNPNNPTGYLYSKEELMQLRDLILKYDLFLFADEVYREFVYDGKEHFSV
                     MNLDGLDEHVVLMDSISKRYSACGARIGALISRNNKVMETAMKFAQARLSPPTLEQIL
                     AEAFLDVPETYFQEVGKEYVERRDLCVARLNAMDGVMCPTPGGAFYAIARLPIDDADV
                     FCQWLLESFNHNNETVMLAPATGFYSTPGLGKNEVRIAYVLNKQDINKAMDCLDAALK
                     VYPGRTAIIQNAEVNA"
     gene            9927..11336
                     /locus_tag="PLM90_06200"
     CDS             9927..11336
                     /locus_tag="PLM90_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ88997.1"
                     /translation="MRTLCLSICLLLSATAFSQQIPADLEGKWLGKINAGIELRLVLN
                     FTIHNDSLFTTLDSPDQGVNDIPTSSTTYTAGSNEIQVEIPLIKAQFKGVYTAATQSI
                     SGIFIQGAPMPMTLTKVTEVKGIVRPQEPVKPYPYKEEEVTFKNKQTNDVTLAGTLTI
                     PEGKGPFTAVILVTGSGPQDRNEALLGHKPFLVLSDYFTRKGIIVLRYDDRGVAQSTG
                     DHETATTADFASDANAAVDYLLSRKDLNIGKIGIAGHSEGGVIAPLAASKNKQIDFIV
                     LLAGTGIPGDSILNLQADLIARAMGAPEDALQATEKYRRDIINIAKANDDPAMIKSAI
                     IDYNKNLIKNSSAEYLNLLSMTETDTLAAVNFYANAWMHYFLNYDPQPTLRKLKIPVL
                     AVNGTHDLQVPYKENLSAIEAALKEGKNKQYSTMAFDGLNHLFQQSATGSPSEYSHIE
                     ETFNEQAMEAIATWILTLK"
     gene            11427..12155
                     /locus_tag="PLM90_06205"
     CDS             11427..12155
                     /locus_tag="PLM90_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88998.1"
                     /translation="MELATLKLGNLIIGEPMTSLTDILLAVTSFVLVARIRNCLNESF
                     FNNAWRMFFLCMGISTSIGTIAHAINGSSAQQLFNILWMAMTFASSTAVFYAVHATIR
                     FMRSSGQWRKIFMAINVIALIVFLLYTAAFNNFEIFKIHAAVGVVIIFLTHTIAWLRN
                     HHGSGWVAVGMGLSILTVVIHSMQLSISKWFNYKDISHVIMLVSLICIYNGIHAMSDN
                     LQLSVFRSRTKESENNFNGAERAL"
     gene            complement(12158..12727)
                     /locus_tag="PLM90_06210"
     CDS             complement(12158..12727)
                     /locus_tag="PLM90_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ88999.1"
                     /translation="MQEIEPYYNWREYYVSEEDENSPFFGREYSEFEFSNTIYNFFIH
                     PQWDEFGSATLYMKIIFADYDSGVAIMEFIGEWNDCLYNDIMFLKRDVIDELKYAGIH
                     KFILIGENVLNFHGGDEDYYEEWFDEIEDGWVACLNFREHVEAEMISAHIDRYFLIGA
                     PFNDINWRKYKPQALAFLVEQYMQKKIGA"
     gene            complement(12813..13619)
                     /gene="murQ"
                     /locus_tag="PLM90_06215"
     CDS             complement(12813..13619)
                     /gene="murQ"
                     /locus_tag="PLM90_06215"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HMZ89000.1"
                     /translation="MDKITEQPSRYRHLEKMSTTELLSNINTEDKSVPVAIEKVLPQI
                     AQLVDVVADKMLAGGRLFYIGAGTSGRLGIVDASECPPTFGVPHGLVIGLIAGGDAAI
                     RKAVERAEDDMEQGWLDLMAYQIGPNDVVVGIAASGTTPYVIGALKTCREKGITTACI
                     TCNTGSPVAMQSDFPIEIVVGPEFVTGSTRMKSGTAQKLCLNMLSTAVMIRLGRVEDN
                     KMVNMQLTNDKLVDRGTKMLAELLDIEYEEAKKQLLLHGSVKKAVQAHSK"
     gene            13704..13928
                     /locus_tag="PLM90_06220"
     CDS             13704..13928
                     /locus_tag="PLM90_06220"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HMZ89001.1"
                     /translation="MSIEKHTYTNGEVTIVWQPKLCIHSGNCVRGLPAVFNNRERPWI
                     KAEGADTEAIIEQVKKCPSGALTFILNTNK"
     gene            13941..15047
                     /locus_tag="PLM90_06225"
     CDS             13941..15047
                     /locus_tag="PLM90_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89002.1"
                     /translation="MREIVQMQKAKEIISTYDGKQPLQLHLKNYFRLHKEMGSRDRKL
                     YTQLAFGYYRQKGMAPHNDRFLVDAVANMEQFHPFYAYWSAEVPEVAGIAWNQYFPFS
                     DHISDSINIEELEKSLHTNPATWIRCKKAHITDILDELSAKGFVFDRDDTCIRFDQPV
                     PLQELETFEKGYFEIQDIASQQTGTLMHPEKNAAWWDCCAGSGGKSLLLLEKEKQLQL
                     FVSDNRESILHNLQDRFSRAGIRQFAAKLIDLETADDKALQGLPDMQGIIADVPCSGS
                     GTWARTPERMAHFDGTQLEKFVALQHKILDRTVNKLLPGGTYLYLTCSVFTDENEQQV
                     AYLQQQHQLTLQEMHYFQYSSQGGDTLFGARFTK"
     gene            complement(15060..16721)
                     /gene="ftcD"
                     /locus_tag="PLM90_06230"
     CDS             complement(15060..16721)
                     /gene="ftcD"
                     /locus_tag="PLM90_06230"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HMZ89003.1"
                     /translation="MRQIIECVPNFSEGRDMQIIRQITDAISSVEGVTLLDVDPGKAT
                     NRTVVTFVGDADAVVEAAYRGIAKAAEVIDMRKHHGEHPRMGATDVCPFVPVANATME
                     DCIACAKKLGERVGSTLNIPVYLYESAATSPERKNLATIRAGEYEGIEKKILDPMWKP
                     DFGPAQFNPQTGNCIIGARDFLVAFNVNLNTTSVRRANSIAFDVRENGRVDPSGAKDA
                     QGEALRIPGACKSVKAIGWFIEEYGIAQVSMNLTNIRDTPVHIAFDACVNSAASRGMR
                     VTGSELVGLIPLQCLIDAGKYFLEKQERSIGVSEEELIKITVKSMGLDELAPFNPADK
                     IIEYKLGSTTGKLVSMNLRAFANETASESPAPGGGSIAAYCGALGISLATMVANLSSH
                     KKGWDARWKEFSDVADKGQQLKDRLLFMVDEDTAAFNKIMDAFGLPKATPDEKSARKK
                     AIQDATVYAIEVPFKVMQLSFESMALIKAMAENGNPNSVSDAGVGALCARTAVLGAFL
                     NVKINASGLDDKALAANKIAMAQKIADDAMAMEKEILSIVDSKIG"
     gene            16881..16953
                     /locus_tag="PLM90_06235"
     tRNA            16881..16953
                     /locus_tag="PLM90_06235"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16914..16916,aa:Gly,seq:gcc)
BASE COUNT         4981 a         3832 c         3470 g         4781 t
ORIGIN      
        1 gcaatatccg gattatgcaa aaaagatgtg aagcgctgcg tatcgtgact atcatacaaa
       61 ttctgcatta cgtaagtgaa atccggtcta taggcctgac gtaaatcagc aagtaaggaa
      121 tcgaaggtgg ttacagaaat gcgcgattgt ttgtttataa aaaactcact tgcaatcaca
      181 ccaaatccat aattcattac tgcatcaaat tcatctcctt gtaaataagg tgcaactact
      241 tcctttttat caattacttc cgcagttaaa tatgcttccg gattaataga tttcacccac
      301 aagcgccatg atttccaaaa atcgtgatcc acacaaaaag ccacatccaa ccgccatccg
      361 tctataccgt ttgctgcatt cccatcaggt gccatccatc ttttggtgca gtcaaaaata
      421 tattgtttcg gaccggccac aattcccatc gaatcttctt ttatttcagg gagatccgga
      481 acaccaaacc aaccctgata tttaaatgtg gttcctgcaa ggctatcatc ccaactataa
      541 atggtaaacc aatctttgta tttagaatcc tgctgatttt ttaccacatc ctgaaaagca
      601 aaactattta tgcccatatg attaaacacg ccgtcgaaaa tgattttaat accgcgtttg
      661 tgacaggagt ttattaattg cagtgccagt ttatctgctg ccgtccaatg ccaggttgca
      721 ggatttatcg ggtcttcact tgctatcaac tttttatcac cctcaggatc tggaccaaaa
      781 ttcggatcta catgatgata cattaccgca tcatacttat gcaaactcgg cgcccaaaaa
      841 acaggattca gataaatggc attaatgcca agactgtcga tataccctaa gcgatcgata
      901 atcccttgca aatcgccccc gtatcgcctg cgttgaacat tgtagttaaa atcttgtcca
      961 ttggcgagtt cccacggttg catggcatac cagtctgatg tccatggcat gatttcccag
     1021 gctgcagtat cattatgcgg ccagcaacct ttgatatcgg caagcgtggg atcgtttgaa
     1081 gggtccccat tgcgaaagcg gtcggggaaa atctgatacc atattgcctc ttttgcccat
     1141 gcaggggtct gagcacctga tggaagcgcc cttagcatac aaattgatgc aattaacaga
     1201 agtttatgtt tcacagtgta aatttgcata aataagtgct gcattgaaaa gttcgttact
     1261 catattttat ctgtgtctgc tcagcacctt cgttgcaaag gctcaaatga caccacccta
     1321 tagtatttta tatgacgaca cacaagtgaa caagatctat atcaccatgg atatagattc
     1381 gctttcagaa atgtacgatg aacttgaaaa cgaacatgag tatgcagtgc aatttgtttt
     1441 cgacagaggt acacaaaccg acaccttaca ggatgtagga tttagattga gaggtaatac
     1501 ctctttatcc agtgcaaaaa aatcatttaa agtttcgttc aatacttaca atccgggtcg
     1561 cacgtttgaa actgccaaga aattaaatct tatcggcaat cacaacgacc ctactatggt
     1621 tcgtgaaaaa acgtatttcg acatttataa tcgcctaggg cttcccatgc gcagagtggg
     1681 atttgctgaa gtgtatatta acgatcaata ttttggattt tataccgtag ttgaagaata
     1741 tgacgatatc tggtgccgcg accgctatgg tgatgatgca ggtgctctgt tcaaatgttt
     1801 atacggcagt acatttacct ataatggtga aaatacaggt gcatacaaca catacgaaca
     1861 acaatcaaat gaacaagtga atatgaaagg cgaattggct cacatgttcg atgtattgaa
     1921 taacacaccc attgccgatc tcccttgtgc tttcgaaccc ttatttgatg tagatcaatt
     1981 tttaaagatc tatgccttag atatctctac cggtcactgg gataattatg gcgccaatca
     2041 aaataattat tatctctacc atgatttaaa atcagggcaa tataaattcc tgagctatga
     2101 ttgcgataac gtccttggca tcgattggtt cggaattgac tggggaacac gcaatgttta
     2161 cgactggaat tttgatggcc gaccattagt tgaacgattg ctgcaagttg atgtatatcg
     2221 cgaacgtttc agctattatc tcaacctcat tgccacttat ggcatggata ccgattattg
     2281 gtatccgcgt ttagacgaca tccgcgacct tatcgctccg tcagcattag ctgatattta
     2341 tcgcaccatg gattatggat attcgtatgg cgattttctt gatggttttg atacagatga
     2401 tatagacgga cattgccctt acggtatcaa gaattttttc gacgcacgca tcaacagcac
     2461 acttgatcag gtggaattaa gtgatatcgc acctatcatt catttccccc aacaagatca
     2521 tatgctttta gaacatggtg attatttaaa tatcactgtt ttcattgaag aagatgtttc
     2581 cgtatccgct gttcagttat tgtatagtaa taatgaaact agttacagca ctgcagattt
     2641 atatgacgat ggtgcacatg gtgatggcat tgcgggtgat aacatttatg gtaacaccgt
     2701 aattgtacct atggatggca ccacttttta ttatcatatt atcgcatctg ataatacagg
     2761 aaatgaaagt gtttatcctg tttgtgaaaa catcgcacgc acaatcggat atacaacacc
     2821 aacattacat atcaacgaag tgatgcctga taatgtaagc acggtgcccg atggagccgg
     2881 tgaatttcct gattttatag aattatataa ttacggttta tatgccattg acatcagcaa
     2941 tttatttatc accgatgaat tggacaggcc aacaaaatgg cgactgcctg atatgatgct
     3001 tccttttgac agttatgtgt taatctttgc cgataatgaa acggaacagg gcctcaatca
     3061 tgctaccttc tctatcgatg cagataagga tgacttattt atttatggcg atgtcactac
     3121 cgattattat ttaatcgatt ccacctcatt caagaatatt ccacctgatg tttcatgggg
     3181 cagattacca aatggaactg gtccatttac gaatttacca gttgcttccc cgggagctta
     3241 taatacggtg gacaccgcct ccgtgccgga tttgttcgaa gtaatactga ccaataatcc
     3301 gggaacgcat acttctgagg taatcattac caacgatatt gcccaaacca ttacactcca
     3361 tattttcaac atggcagggc aggaagtatt taaagaagca cctgccacat tgcccgccgg
     3421 cacctatagt tacagcattc ctgcaaatcg cctgggttcc ggtgcatacc tgattcgtat
     3481 tggccatggc gataaggtta caatgattac atactctgta ttataatgat aattgccaat
     3541 tgtcggctaa agccgtaaat ttgctgcctc aaaacaatta ttatgggacg cgcattcgaa
     3601 tatcgcaagg aaagaaaatt caaacgctgg ggtgcaatgg ctactgcctt tacccgtgta
     3661 ggacgtgaaa ttgccatcgc tgtaaaaaac ggcggaccta atcctgaagc gaatcccaag
     3721 ctgcgtattg ccattcagaa tgctaaggcg gtacagatgc cgaaagaccg tgtagaagct
     3781 gctatcaaac gcgcatcaag caaggatgaa aaagattttg aggaagtggt atacgaaggt
     3841 atcggccccg gtggtgttgc catcatggtt gaatgtgcta ccgataatac tactcgcacc
     3901 gttgcaaatg ttcgcgtact cttctctaaa ggaggaggtg ctttgggtac cagcggatca
     3961 gttggttatt tatttgaacg aaaaggtatt ttcaaagttg aatccggaaa gattaaactc
     4021 gatgttgttg aattagacct catcgatggt ggtgccgaag atatttatga agaagatgga
     4081 actgtttaca tcgaaacatc tttcaacgat ttcggaaata tgcagaagaa acttgatgaa
     4141 ctgaatatcg agatcagtgg tgccgaatta ttgcgtattc ccatgaataa aaatgcggtt
     4201 cctcccgacg ctgaaaaact cgtgcacaat ctcatcgaaa aactcgaaga agacgaagac
     4261 gtagtgaatg tattccacaa tatggtggaa accatggagt aagttttttt tcgcgctaag
     4321 acgcaaagtt gcaaaggcgc aaaacaaatt ccccaaacac acgagtcacg tattacccca
     4381 aactcacgag tcacgattca cgattcacgt attaccccaa actcacgagt cacgattcac
     4441 gtattacccc aaactcacga gtcacgattc acgagtcacg attcacgtct taccccaaac
     4501 tcaccacccg cctgctcggc gaggtaaact cacccactca ccactcacca ctcaccactc
     4561 acccactcac cactcaccaa aaaaaaagcc cattcttacg aatgagcttc ccgactgaat
     4621 agtcaataag attattgcgc aagtttaacg ttaactgcgt ttttgccttt gcgaccgtcg
     4681 atgatgtcaa aagtaacaag atcgttttgc ttaaccttgt caaccagacc ggtctggtga
     4741 acgaaaattt cttcgttggt accatcctta gtgatgaaac cataaccctt agtggcattg
     4801 aaaaatttga ttttgccggt ttccattgta ataataatta gtagtaaaaa aattggttaa
     4861 cgttcaaaga tatataaact ttaagaaatt gcgggatact gccccggatc cacttcgctg
     4921 accatagcat agatagcttc gaacacatcc tcttcgcttg gcttacagaa ataatcacca
     4981 tcgctggcat aagccgacct gttggctttc gcgctcaaac atttcgggaa ggcatcaagg
     5041 gagcggaata gggtttcatc tgcaaatacc tgctgcatca tataggccga agctccaccc
     5101 ggaacgtctt catctacaaa cagtacccta tttgttcgac gaacagatgc cgcaataatg
     5161 ccctgaacat caaatggcat cagtgtttga gcatctatga gttcaattga catacccagc
     5221 gcagccagtc tgtcgcaggc ttcttctacc acacgaagag tagaaccata gctgacaaca
     5281 gtgatatctg ttccattcct tagaatctca ggcacaccca gcggcacagt gaacgtattg
     5341 atattcaatg gcagcgcctc tttcaggcga tagccgttca agcactccac cacgatagca
     5401 ggctcatcac tttgaagcaa agtattatac atgcccgcag cctgtgtcat atttcttgga
     5461 acacaaaggt gaacgccctt gcaggtattc aaaatcattc ccataggtga accggtatgc
     5521 caaatgcctt caagcctatg tccgcgtgtg cgaataatca aaggcgcctt ctgcccaccc
     5581 gccgtgcgat actgcaaagt cgccaggtcg tcgctgatag gttgtaagcc gtaaatcaga
     5641 taatcgaggt attgtatctc agcgatggga cgcatgccac gcaatgccat gcctattccc
     5701 tgcccaataa tggttgcctc gcgaataccc acatcaaaaa tgcggtgttc tccatgtttc
     5761 tcctgcagtc ctgcaaaacc ctgattcaca tcaccaatct tgcccacatc ttcaccaaag
     5821 gcataaatgc gggggtttgt agagaaatgc atatcaaagc acttattcaa cagttcaaac
     5881 ccatttacca tcgtcggctc ctccggatat tgtgcgggaa tcaccggaat atgttgaatg
     5941 gcatgtgctg aattgctgaa gagatttcca ttatagcgtt ggtcattcaa tgccgcatag
     6001 tcttcagccc atgcttgtaa agctgcccgt tcaatggtct gttctccggc tgtagcatgc
     6061 aatgccaatc gagcggcacg cataatatct ttacgaacag catccatcgt atctttcaac
     6121 tcagtaatga ttgctgctat ttgtccggag gcattacttg aattccggag gttttccagt
     6181 agcgaaaaaa ctacatccat gtctgtcttt atagacatca gatgtgcatt ccatgcagct
     6241 ttttgatgct gacgggcctc gtcttttgct ttggcaacaa tatcgttgag ttcttcttct
     6301 gttgcaacac tggcggctat catccattta cgcatctgac gcacacagtc ccattcggcc
     6361 tcccattcca ggcgctcctt acttttgtag cgctcatgtg aacctgaagt agaatgtcct
     6421 tgtggctggg tgatttcagt aacatgaaat aataccggaa tatgttctct gcggcaaagg
     6481 tcaataccct cctcaaaggt ctgaaccatc gaagggtagt cccagccttt tacctcatag
     6541 atgcgcacgc catggccatc ttcgtcgagc tgaaaccctg agagtatctc agaaatgttc
     6601 tgcttggtag tttggtattt ttgcggcacg gagataccat aaccatcatc ccacacaaaa
     6661 accgccattg gtatcttaag cacacctgca gcgtttatcg actcccagaa aaggccttct
     6721 gaagtactcg catcgccgat agtggtgaaa actacttcgt tgccattatt gctaaaacct
     6781 tgtgtatgca cccccggatt actgcggtag agtttagatg ccaatccgag accaacagct
     6841 ctggccatct ggcttgcagt tggtgaggtg tcggaggaac tgttttttac atttacgagg
     6901 tcattccagt tgccattggc atcgatcgta cgcgtggcaa aatgtgcatt catctgcctt
     6961 ccgcctgaaa acgggtccat ggaaatatca ggaatggcat acaattgggc aaaaaactgc
     7021 ataggcgttg ccattccgga tgcaaacatg aaagtctgat cgcggtaata acccgaacgc
     7081 cagtcgccct ccttaaaaac ccttgccatg gccacctgag caacttcctt accatcgccg
     7141 aatatgccga atttggcctt acctgtaagc acctccttgc gacctaaaag gcttatctca
     7201 cggctcagac aggcagtaac aaagtcgttg atgacattgg ccctgaatga ttcaaaggtg
     7261 agctgggtag tggacaatga ttcggacata agtgtaaaat cgtttggtcg caaaaatacg
     7321 aaattcgacc tcccctcccc atttctgaac gatttttgcc cgaaaaacgc aagatggtat
     7381 aattcttgta tgttttttca ggctgcaaat atttgcggca gttgcttaga tttggcctcc
     7441 cggtcggcaa gcagctattg accaaccgta ataaaaacca atttgaatat gaaaaaattt
     7501 acttacacac tcgctttgac gatgctgatc gcaacagctt catttgcgca aacttcaaca
     7561 acaccacctg caggatcatc agaagctacc actccggtag taaatcctaa tgcgggcgaa
     7621 tttcaatggt ctgaagaaac ttacgacttc ggcactattg ctcaaggcgt tccggtgaaa
     7681 cataaattcg aattcaccaa taccggtaaa gaacctatta ttgtaagtaa cgtggtaaaa
     7741 acatgtggtt gtacagttac tgactggaca aaagaacctg tattacccgg acaaaaaggt
     7801 caggtaattg ctgaattcaa tgctgcaaaa gaaggtccat ttacaaaagc catcacagtg
     7861 cagtcagatg caaaaacacc atcagtgaaa ctctacttca aaggcactgt tcaaaaatca
     7921 gaacaagccg gaagcgttcc tgaaaacaac aatattttta accagggtgg taactgatca
     7981 tccacagcct aaatattaaa gaacatgaaa aaagtattat cgattgttgc atttttagct
     8041 gttaccggcg caatgacggc acaaacagct acaggcacag gaactaccgc tgcagtaagc
     8101 gaaaatgctg ctgaattcca gtttgttacg gaggtttttg atttcggtca aatcccacaa
     8161 ggagttcctg ctgaagcgcg tttcgaattc acaaacacag gtaaagaacc gttgatcatc
     8221 accgatgttc aaaaaacatg cggatgtacc aaaaccgatt ggagtaaaga acctatcgct
     8281 ccgggtgcta aaggatggat catcgccgaa tataatgctg caaatgaagg ctcatttact
     8341 aaagccatca ctgttttctc aaataccaaa acgccaaaca tgaagcttac cttcaaaggc
     8401 acagtggtaa aagatgaatc aggcagcgtt cctcagcaaa aatcgatttt cgataattga
     8461 tatttacctg aacgttatct gcgaaaagga ggctgtctga aaaggcggcc tctttttttt
     8521 gcagttgatt gagctgcaag aacttacaag cccctccttg aatcacaggg cgttcatttc
     8581 ccaaaaaccg cctacctttg ccttatgcca catatttcgc aacgaggggc tgatatgcct
     8641 gcctcaccga ttcgcaaact ggttccattt gcagaaaaag caaaactcag aggcgtaaaa
     8701 atctaccatc tgaatatcgg tcagccggat atcgttacac ctccgcaaat gctggatgcc
     8761 gttaagcgca cagacctgaa agtgattgaa tacagtcact ctgcaggatt tgaatcttat
     8821 cgtcgcaaat tggtgactta ttacgaaagc agaggtattc atattaatca tcatcagatc
     8881 atgatcacta ccggaggcag cgaagccatt ttattcggta tgatgagttg tttggatgcc
     8941 ggggatgaag tgattattcc cgaaccgtta tatgcaaatt acaatggttt cagcacggca
     9001 ggaaatattg tgatcaaacc aattacctca catatagaaa acggtttcgc actgccaccg
     9061 attgcggaat ttgaaaaact gatcacgccg aaaacaaagg caattcttat ttgcaatccg
     9121 aataatccga caggatattt atatagcaag gaagaattaa tgcaattgcg cgatcttatt
     9181 ttaaaatatg atttgttcct ttttgccgat gaagtctatc gcgaatttgt gtacgatgga
     9241 aaggaacatt tttcggtgat gaatctcgac ggactggatg agcatgtggt actgatggat
     9301 tctatttcta aacgatacag tgcttgcgga gcgcgaattg gtgcgttaat atcacgcaat
     9361 aacaaagtga tggaaacagc catgaaattt gcacaagcgc gtttatcacc tcctacattg
     9421 gaacaaattc ttgctgaagc attccttgat gtaccggaaa catattttca ggaagtgggg
     9481 aaagaatatg tagagcgccg cgatctctgt gttgcgcgat taaatgcaat ggatggcgta
     9541 atgtgcccaa ctccgggtgg tgcattttat gctattgcac gtttacctat cgacgatgct
     9601 gatgtttttt gtcagtggct gctggaatca ttcaatcaca ataatgaaac agtgatgctg
     9661 gcacctgcaa ccggatttta ttctactccc ggattgggaa aaaatgaagt gcgtattgct
     9721 tatgtgttga ataaacagga tatcaataaa gcaatggatt gcttggatgc tgcgttaaaa
     9781 gtttaccccg gcagaaccgc aatcattcaa aatgccgaag taaacgcctg atagcaggct
     9841 tacgtccgtc gaagaattaa tgggtttcat ctaaagtgtt ggggcgtgat ggcttttgcc
     9901 gatacttttg tacaaaatat tacatcatgc gtaccttatg cctttccatt tgcctgttat
     9961 tatcggcaac tgcattttca caacaaattc cggccgatct ggaaggaaaa tggttaggga
    10021 aaattaatgc gggaatagaa ctgagactgg tattgaattt taccatacat aacgacagtc
    10081 ttttcaccac gcttgatagt cctgatcagg gagtaaacga catccccaca tcctcaacaa
    10141 cttatacagc aggatcaaat gaaattcagg ttgaaattcc attgatcaaa gcacaattta
    10201 aaggtgttta tactgctgca acacaatcta tttccggcat atttattcaa ggtgcaccaa
    10261 tgccgatgac gctgactaag gtgacagagg tgaaaggaat tgtacgccct caggagcctg
    10321 ttaaaccata cccttataaa gaagaagaag taacattcaa aaacaaacaa accaatgatg
    10381 ttacactggc cggcacactc acaataccgg aagggaaggg gccttttact gctgttattc
    10441 tggttaccgg ttcaggaccg caggatagaa atgaggcctt gctgggacat aaaccatttc
    10501 tcgtattaag cgattatttc acacgcaagg gcattattgt attgcgttac gacgatagag
    10561 gtgtagcaca atctaccgga gatcacgaaa cagcaacaac agcagacttt gcatcagatg
    10621 ccaatgctgc ggttgattat ttattatcac gtaaagatct caatatcggc aagattggta
    10681 ttgccggaca tagtgaaggt ggggtgatag cacctttggc agcatcgaaa aataaacaga
    10741 tagatttcat agtgttgctg gcaggaacag gtatacccgg cgattccatt ttaaatctcc
    10801 aggcagatct gatagcaagg gcaatgggtg caccggaaga tgcgcttcag gcaactgaaa
    10861 aatatagaag agatatcata aacattgcta aagcaaatga tgatcctgca atgattaaat
    10921 cagctatcat tgactataat aaaaacctca ttaaaaattc ttcagcggaa tatctgaatc
    10981 tgttgtccat gacggagaca gatacactgg cagcagtaaa tttttatgct aatgcctgga
    11041 tgcattattt tctgaattat gaccctcagc ccaccctgcg caaattaaaa attccggttc
    11101 ttgccgtcaa tggcacgcat gatcttcaag ttccctataa agagaatctt tccgccattg
    11161 aagcagcgct gaaagaagga aaaaataagc aatacagcac tatggcgttc gacgggctga
    11221 accacctgtt ccaacaatct gccacaggct ctccttctga atacagccat atagaggaaa
    11281 cctttaacga gcaggctatg gaggctattg ccacatggat cctaacttta aaataggact
    11341 atttctctgc cacattgcga tttcgtatct ttgttgcctg gatgaggctt tgatttcttc
    11401 cgggaccaaa caatacattg cagcttttgg aactggcaac acttaaacta ggcaatttaa
    11461 ttattggcga acctatgacc tcgctgacgg atattttatt agccgtaacg agttttgttt
    11521 tagttgccag aatacgcaac tgcttaaatg aaagcttttt caataatgcc tggcgcatgt
    11581 tctttttatg tatgggaatt tctacctcca ttgggacaat agcgcatgcc ataaacggat
    11641 ctagtgcaca acaattattt aatattttgt ggatggcaat gacctttgcc agcagcactg
    11701 ccgtgtttta tgctgttcat gccaccatca gatttatgcg ttcttcagga cagtggcgaa
    11761 aaatatttat ggcaataaat gtgattgcgc ttatcgtttt tttattgtat accgccgctt
    11821 tcaacaattt cgaaatattt aaaattcatg ccgccgttgg tgtggtaata attttcctaa
    11881 cacataccat tgcctggttg cgcaatcatc acggaagtgg ctgggttgct gtaggcatgg
    11941 gtctttccat tttaacagtg gtgatccaca gcatgcaact ttcaatatct aaatggttta
    12001 attataaaga catcagccat gtaattatgc tggtgagcct gatctgtatc tataacggca
    12061 ttcacgccat gagcgacaat ctgcaattga gtgtattccg ctcacggact aaggaatctg
    12121 agaataattt taatggcgct gaaagggctc tttgaaatta ggcacctatt ttcttttgca
    12181 tgtattgttc taccaaaaag gctaatgcct gtggtttgta tttacgccag ttgatgtcgt
    12241 taaacggagc acctatcaaa aaataacgat cgatgtgggc acttatcatt tctgcttcaa
    12301 catgctctcg gaaattcaaa caagctaccc aaccatcttc aatttcatcg aaccattctt
    12361 catagtaatc ttcatcgcca ccatgaaagt ttaaaacatt ttcaccaatc aaaatgaact
    12421 tatggatgcc tgcatatttt agctcatcaa ttacatcgcg ttttaaaaac atgatatcat
    12481 tgtacaggca atcattccat tcgccgataa attccatgat ggctacacct gaatcataat
    12541 cggcgaaaat aattttcata tataatgttg cagagccaaa ctcatcccat tgagggtgga
    12601 tgaaaaaatt atatatcgta tttgaaaatt cgaactcact atattcccta ccaaagaaag
    12661 gactattctc gtcttcttcg ctcacataat attcacgcca attgtaatat ggttcaatct
    12721 cctgcatacc tgataacgcc ggaaaaataa tccgtatttc taaaacaaaa aaataaaatt
    12781 aatgcaacta aaaaaaggaa gttaaaatcg acttatttgc tatgtgcctg tactgctttc
    12841 ttaacacttc catgcaatag caattgcttt ttcgcctcct catattcaat atccaataac
    12901 tctgccaaca ttttggttcc gcgatcaacc agcttgtcgt tggttaattg catgttaacc
    12961 attttattgt cttctaccct gcctaaacgt atcatgactg ccgtagagag catattgagg
    13021 cagagtttct gagcggttcc gcttttcatg cgtgtagagc cggtaacaaa ctcgggacct
    13081 acaactattt cgatgggaaa gtctgattgc atggcaaccg gtgaaccggt attgcacgta
    13141 atacaagccg ttgtaattcc cttttcgcga caggttttta atgcaccgat cacatatggt
    13201 gttgttccgc tggcggcaat gccaacaacc acatcatttg gcccgatttg ataggccatt
    13261 aaatcgagcc aaccctgctc catatcgtcc tcggcccgtt caacagcctt tctgatggcg
    13321 gcatctcctc ctgcaattaa tcctatcacc aatccatgcg gaacaccgaa agttggcgga
    13381 cattcactcg catcaacaat gccaagacgg cctgatgttc ctgcaccgat gtaaaataac
    13441 cttccaccgg ccagcatttt atcagctact acatcaacca attgagcaat ttgaggcagc
    13501 accttctcta tggcaaccgg aaccgattta tcttccgtat tgatattgct caatagctcc
    13561 gtagttgaca tcttttcgag atgccgatat cgcgatggtt gctcggttat tttgtccatt
    13621 tgtgatacaa aataaatcaa atttgcatta tcctcaggcg atttgattgg gttttatgta
    13681 tacttgctta ataatcaggt actatgtcaa tcgaaaaaca tacttatacc aatggtgaag
    13741 tgaccattgt atggcaacca aaactctgca tccattcggg caattgcgta agaggattgc
    13801 ctgctgtatt caacaatcga gaacgtccct ggataaaagc agaaggcgcc gacacggaag
    13861 ctattatcga gcaggtaaag aaatgtccct caggtgcatt gacttttatt ttgaatacaa
    13921 acaaataata tcgtttcaat atgcgcgaaa tagttcaaat gcaaaaagca aaggaaatca
    13981 tttctactta tgatggaaaa caacctttgc aattgcacct taaaaattat ttccgcctgc
    14041 ataaggaaat gggcagcaga gatcgcaagc tttatacgca acttgccttc ggatattacc
    14101 gacagaaagg catggcgccg cataacgata ggtttcttgt agatgccgtt gccaatatgg
    14161 aacaatttca tccgttctat gcttattggt cggcagaggt tcctgaagtt gccggcattg
    14221 cgtggaatca atactttcca tttagcgatc acatttccga ctcaataaat attgaagaac
    14281 tggaaaaaag tctacataca aatcctgcaa cctggatacg atgtaaaaaa gcacatatta
    14341 cagatattct cgatgaatta agtgcaaaag gatttgtatt tgaccgcgat gacacctgca
    14401 ttcgttttga tcaacccgtt ccattgcagg aattagaaac ttttgaaaaa ggatattttg
    14461 aaatacaaga catcgcttcg cagcaaacgg gaacactcat gcatcccgaa aaaaatgctg
    14521 cgtggtggga ttgctgcgcc ggttcaggag gaaagagttt attgttgctt gaaaaagaaa
    14581 aacaattaca gttattcgtg agcgataata gggagagtat tttacataac cttcaagaca
    14641 gattttcacg cgccggtatt cgtcaatttg cagccaagct gatcgatttg gaaactgccg
    14701 acgacaaagc tttgcaaggt cttcctgata tgcagggaat cattgctgat gtgccatgca
    14761 gcggatccgg aacctgggca cgcacacccg agcggatggc acattttgat ggcacacaat
    14821 tagagaaatt tgttgcgctg caacacaaga ttcttgatcg aacagtaaat aaattattgc
    14881 cgggtggcac ctatttatat cttacctgct ccgtatttac cgatgagaat gaacagcaag
    14941 ttgcctatct tcaacagcaa catcaattga cattgcaaga gatgcactat tttcaatatt
    15001 catcacaagg aggagatacc ttgtttggtg caaggtttac aaaataacat attatttaat
    15061 caccctattt tactgtcaac aatggataat atttcttttt ccattgccat tgcgtcgtct
    15121 gcaattttct gagccatggc gattttattt gcggcaagtg ctttatcatc gagacctgat
    15181 gcattaattt ttacattcag gaaggcacct agtacagcag ttcttgcgca caatgcacca
    15241 acaccggcat cgctcactga attcggattt ccattttcag ccatcgcctt tatcaatgcc
    15301 atactttcga aactaagttg cattacttta aatggcactt ctatagcata aacggtagca
    15361 tcctgaatgg cttttttcct tgccgatttc tcatccgggg tagcttttgg caagccgaag
    15421 gcatccatga ttttattgaa ggcagcggta tcttcatcca ccataaataa aaggcgatct
    15481 ttcagttgct gtcctttgtc tgcaacatct gaaaactctt tccaacgagc atcccaacct
    15541 tttttgtgtg acgataaatt ggcaaccatg gtagccagtg aaatacccaa tgctccacaa
    15601 tatgcagcga tagagccacc accgggcgcc ggactttcag atgccgtttc attggcaaaa
    15661 gcacgcaggt tcatcgacac cagtttgccg gtagtagagc cgagtttata ttcgataatt
    15721 ttatctgccg gattaaacgg agccagctca tcgagaccca tacttttcac tgtgattttt
    15781 attaattctt cttcgctgac acctattgag cgttcttgtt tttctaagaa atattttccc
    15841 gcatcaatta aacattgcaa aggaattaat cctactaatt cacttcctgt tacgcgcatt
    15901 cctcgtgatg ctgcgctgtt cacacatgca tcgaaggcta tgtgaacagg cgtatcgcgg
    15961 atattggtga ggttcataga aacctgtgca attccatact cttcaataaa ccaaccgatc
    16021 gccttcacac ttttgcatgc accgggaatg cgcagtgctt caccttgtgc atctttagca
    16081 cctgatggat ctaccctgcc attttcacgc acatcgaacg caatggagtt agcgcgacgt
    16141 acagaagtag tatttaagtt tacattaaag gcaacaagaa aatctcttgc gccgataata
    16201 caatttccgg tttggggatt gaattgtgcc ggaccgaagt cgggtttcca catgggatct
    16261 agaattttct tttcaatacc ctcatattca cccgcgcgaa tggttgccag attttttctt
    16321 tcaggagagg ttgcggcact ttcatacaag taaacaggga tattcaatgt agatcctacg
    16381 cgttcaccta atttttttgc gcaggctata caatcttcca ttgtggcatt ggcaacgggc
    16441 acgaaaggac atacatccgt ggcgcccatg cggggatgct cgccatggtg tttgcgcata
    16501 tcaattacct cagcagcctt ggcaattcct cgatatgcag cttcaacaac ggcatcggca
    16561 tcgccaacaa aggtcactac agttctattg gtagctttcc ccgggtcaac atccaggagg
    16621 gtaacaccct caacagaaga aatcgcatcg gtgatctgac ggatgatttg catatctctg
    16681 ccttcgctaa aattaggcac acattctatg atctggcgca tggtacaatt ttgctcaaat
    16741 atagggcata agcgccatag gcattgatga tattaattat cgtaaaccat tgaccggcgg
    16801 cgacttacag atttttttcg cttacatttt tgcggatacc ccgaatcctt atatatttgc
    16861 actcccgatt aaagggaaat gcgagagtag ctcagctggt agagcacaac cttgccaagg
    16921 ttggggtcgc gggttcgaat ctcgtctctc gctcatacag cacggcaaca gccgtgctta
    16981 ccttttttaa ggcgcccggg tggtggaatc ggtagacacg caggacttaa aatcctgtgg
    17041 gcagtaatgt ccgtgcgggt tcaa
//
LOCUS       DAOQOU010000050        16891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1707_length_16891_cov_7.284212, whole genome shotgun sequence.
ACCESSION   DAOQOU010000050 DAOQOU010000000
VERSION     DAOQOU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16891
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_16891_cov_7.284212"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1338)
                     /locus_tag="PLM90_06240"
     CDS             complement(205..1338)
                     /locus_tag="PLM90_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ89004.1"
                     /translation="MLSNIIIKPLTHRGGEHYALFFSYDKKLVDQAKKAGCRFSFQNK
                     CWYLPATKENLHKLFTVFKGVAWLDTKAIKHNSGIAKPVEKKNRVSVAEPLPDARLAE
                     LEKLKEWMQHKHYSERTIKVYLDCVRLYFSVNRDKDPHAINREDFISFIREHIIARKL
                     SFTYQNQFVNALKLFLQMVYGLHVNTDFLERPRREHKLPNVLSKQEVLSILKHTDNIK
                     HNLMLRLAYGCGLRVGEVLALRVDDIDRMRRCIQIRLAKGFKDRVVPLPISVEPILNM
                     YIERYQPKYYLFEGEKPNSKYTERSLQLVLKKSVTLSGITKPVTMHWLRHSYATHMLE
                     SGVDTRFIQVLLGHKNIKTTEIYTHVSTHQINAISSPIDSLNL"
     gene            1552..2412
                     /gene="rfbA"
                     /locus_tag="PLM90_06245"
     CDS             1552..2412
                     /gene="rfbA"
                     /locus_tag="PLM90_06245"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HMZ89005.1"
                     /translation="MKGIILAGGSGTRLHPITLAISKQLMPIYDKPMIYYPLSTLMMA
                     GIKDILIISTPYDLPGFQRLLGDGSRIGCSFSYVEQPSPDGLAQAFTLGADFIGNDKA
                     ALVLGDNIFYGSGLGELLTANNNPDGGIIYAYHVSDPERYGVVDFDAAGNVLSIEEKP
                     LQPKSNYAVPGLYFYDNTVVDIARNLKPSPRGEYEITDVNKEYLRQGKLKVSVLPRGT
                     AWFDTGTFDSLMAASTFIQLIEQRQGQNIGCIEEIAYRKKFINAEQLEKIAQPLMKSG
                     YGQYLMRILK"
     gene            2816..3841
                     /gene="galE"
                     /locus_tag="PLM90_06250"
     CDS             2816..3841
                     /gene="galE"
                     /locus_tag="PLM90_06250"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMZ89006.1"
                     /translation="MNTILITGGCGYIGSHTAIDLLEKGFNVISIDNLSRSKTFIADR
                     IREISGKQFHNYRVDCRDLVKMRNIIDQHGKIDGIIHFAAFKSVGESVVKPLVYYENN
                     INSTLNMLRIADEFHIPNFVFSSSCSVYGNTAQQPVKEDAPMATPESPYGWTKLISEQ
                     MIAAHAPNVQTKFIALRYFNPVGAHHGGRIGEIPYSEPENLVPNITQTAIGKKKGFTI
                     FGTDYPTRDGSCIRDYVHVMDIAAAHTAALQYQLSGQQQVPFDVFNLGSGRGVTVLEL
                     IRAFERSTGVSLPFEIGPRRQGDVVAIYANTEKAMQSLHWHPTRSIEEMMHSAWTWEQ
                     TMQRESI"
     gene            3852..4703
                     /locus_tag="PLM90_06255"
     CDS             3852..4703
                     /locus_tag="PLM90_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HMZ89007.1"
                     /translation="MLQTILFDFQAFFGHSPTEAAIIVFNLILIESMLSVDNALVMGT
                     MVMDLPKEQRGKALRYGIFGAYFFRGLALLLATQLIKVVWLKALGGLYLLYLALDYFR
                     SSATNDISDDTLDKKSNWFYKNTVGRIGNFWSTVILVNIMDLAFSIDNVFAAVAYTDN
                     IYLICTGVFIGILAIRFVAQGVIKLLERFSFLNTSAFLIIGLLGLKLLASFICNEYGR
                     ETPFCLFLESEKTDLYFSLITVCIFILPVITSWLFNFPKRSHDHHTDEFVHFQEEADE
                     AEHPKHS"
     gene            4706..5281
                     /locus_tag="PLM90_06260"
     CDS             4706..5281
                     /locus_tag="PLM90_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HMZ89008.1"
                     /translation="MTKGRLEAFSDGVIAIIITIMVLELKVPHSAEWSALHELIAVFM
                     SYVLSFVYVAVYWGNHHHLLHTVKNVSAGIIWSNAALLFFLSLIPFATAWMGENHFAQ
                     NTVIAYSILMNLCGMSYYVLMYMIMQCHSENAELTAALNTQRRKGILSLFLYSFSIGA
                     AFINPMISQVIFVLVAIMWFIPDKNIKRLMR"
     gene            5542..6828
                     /locus_tag="PLM90_06265"
     CDS             5542..6828
                     /locus_tag="PLM90_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89009.1"
                     /translation="MFIQKIVYLDNVFQINNAGVLKPVSGTSTNIQPLIIQHFQLREE
                     GNQHKKQLPLFNPSDFDYYGLYVPESFAYFISPSIGNNPSPDQRMIIIAGADSMVIDF
                     HNIPKTTRYELTILQQIYFMPGYYSLDCSLPARDLKINENEREILYAAVKHGITPETK
                     SILSKAGLLEMALPTDIVKYQKYIPAAITVEQSDAYNINVYFTGNLLTTSNGILITDF
                     QESYRGHWHSTAFSTTFHYIGEESGTSAVFYENQKRVISQTNPMGERNLTDGWYRIGV
                     VNSYGDSIYSKPFCVGLVAANSDSVTTLYNKQRGFPFHNFKDANTINIETAVAPVIID
                     HQPKYVFKYHIVVCNLCLFSDELYGRYDQLAAEEFMHRLAFNDLYINGQPFTGSIEIQ
                     FPIKGATVENGGAWFYTNLIVTNTYINGKLQKHSER"
     gene            7191..7925
                     /locus_tag="PLM90_06270"
     CDS             7191..7925
                     /locus_tag="PLM90_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89010.1"
                     /translation="MRNLLIFGTTMVLMHCALHAQDSSRIVINTININDYTIELVKVQ
                     QIDEVAVKRLNYQAPYSWLYQIYEKDSFIIAVQYFKDSCELYFDSRIKKQGFYYTTHG
                     VKINICDKTVTQIMPPDVSWIKDTFDSITIYPLDSVLDPYDYQGQRTVISNYSEREPE
                     HLNLQHQKDVEFMYRDPRVIGMYQKGSELRLHSALTYYKSGNAQTIYLYYDKFMYEGW
                     FYNVGYSEHGTYNVAKMVWDKHEAAK"
     gene            complement(7982..9118)
                     /locus_tag="PLM90_06275"
     CDS             complement(7982..9118)
                     /locus_tag="PLM90_06275"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ89011.1"
                     /translation="MSRKKVIVLGAGMVGKAMAIDLAKDYEVYSADINTEALQRLEKH
                     GVRTIHADLTSKELIQSLIEDKDLVIGAVPGFMGFETLKTVIESGKNASDISFFDEDP
                     FLLDALAKQNGVTAVMDVGVAPGMDNIILGYHNKRMQVTDFVCLVGGLPAARLWPYEY
                     KAPFSPIDVIEEYTRPARFVQDGKVVVKEALTDPEYMDFTGIGTLEAFNSDGLRSLIK
                     TMPHIPNMIERTLRYPGHIALMKVLRETGFFNKEFIDVKGTHVRPIDVTSKLLFPKWK
                     LGDEEHEFTVMRVIVGGIENDNQVTYTYDLLDRYDPATKTSSMARTTGYTCTAAARLI
                     LEEMYTRKGISTPEFLGEEEAHFHFIMRHLGERGISYQKSQKLR"
     assembly_gap    9454..9553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9724..10107)
                     /locus_tag="PLM90_06280"
     CDS             complement(9724..10107)
                     /locus_tag="PLM90_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ89012.1"
                     /translation="MMIGELRYKDISEKIIGASFEVHKFLGNGFQEVIYQRALAYEMR
                     KAGLEFAREIEQDIYYKELEVPIGTRRADFVVEGKILVELKAIIQLEDVHLAQALNYL
                     KAYKLEVGLLINFGSKSLTFKRLVL"
     gene            11288..12118
                     /locus_tag="PLM90_06285"
     CDS             11288..12118
                     /locus_tag="PLM90_06285"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HMZ89013.1"
                     /translation="MNTFDRMGNGWKISMAGFSVIRKNKQLLVFPILSGAVMLLIIAT
                     FIFAMLGSDFGQVQHLLDNGNPMRYFILFVYYLISYFVVVFFNMALIHCARLYFQGKT
                     PTISDGLNFSMSRIGVIFSWALVAATVGLILRSIQEKVGIVGKIITGLVGLVWSVATF
                     FVVPVLAYENLRPIDAVKRSAQIMREKWGESIGAGFSLLVFNLLGLLLVAVPLFFIGS
                     LISTLASMILAFLGVLIVTTISSAAQTIFISAAYLKINNEEIEGFDDDTIDGLFYRKD
                     "
     gene            12118..12675
                     /locus_tag="PLM90_06290"
     CDS             12118..12675
                     /locus_tag="PLM90_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89014.1"
                     /translation="METPIQSIPPIDRFEPGSFSTTKSRPFPPHVFVVVFIIVVGMFL
                     NMPSPIPALLLAMVDFGLLSMRSGVEVLINAKSYRDFTSIYGIKFGKWKSLGEYDFIL
                     ITRINKRRNAMMNIAYGVSYGDVFFDMDLITKGDRDVLLNRFGEREIVIDKAIQIGQY
                     FDLRVVERVDGDYQVVFEPEKKVVK"
     gene            complement(12765..14207)
                     /locus_tag="PLM90_06295"
     CDS             complement(12765..14207)
                     /locus_tag="PLM90_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMZ89015.1"
                     /translation="MSSVAPFKVCVLQPDYSTSDVDYQYYDPPRNLQPLLPNAIVDHV
                     FLNKLTTYKQLRELKKKGYDIYVNLCEGYLDWSIPSIDVIYCMELLGLPFTGPSSLLY
                     DPTKELMKYVAFTCGVKTPAFVLLQNLDEIDSIASQLQFPLFVKPSKAGDSLGVDEKS
                     LVHDVTALKKKAESIIEEYGPLLVEEYIAGREFTALVIASPDENGAARVLQPIEYIFP
                     EGFAFKTYALKTSELHPNANIPVTDADIIKKLQDATRLIFTGFNGVGYARLDFRMDDK
                     GDLYFLEINFTCSVFYSDGYEGSADYILREDGIGQEGFLRAIIDEGMARYRRTHKPFC
                     LKGNARTGYGIYATRDIEKGEVIFHGEEASQRITTRRHVETHWPEDMQLVFRRYAYPI
                     SNEVFLLWDNDPNAWAPQNHSCEPSTAYDGLDVVALRNISCGEELTLDYATFLDEHME
                     PFECHCGSKNCRGLIKGTLGNSISEMERKK"
     gene            complement(14250..15347)
                     /locus_tag="PLM90_06300"
     CDS             complement(14250..15347)
                     /locus_tag="PLM90_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89016.1"
                     /translation="MAPQLETSDPNLDYYYDFTQSIEEYTKTFRQLQIQWQWQPVNMG
                     NYQNVIADIAAERNAGLSFPVVFNICDGDEVNGAPGVSVVHALEKHKLIYTGADAYFY
                     DITTSKITMKEAFDLAGVPTAKWEAVTDRNQDISGIFQRLGSPLIVKPAVSGGSMGLN
                     VKNVVENETELKEQINKIFDGYRGWQLSAGGIVVEQYINGPEYTTMIIGASDQPRNCV
                     IYTPVERVFHPSLPDKEKFLSFDRLWEIYEDETPMPENDNFYNYTLPARSLISKLKRI
                     SWDAYVSVGGKGYTRVDLRMDKHTGEIGVLEVNAQCGLSEDEDYTSIGAILRMSNKSF
                     AQMVVEIINDATYRWMKKNRVRKLQKSYREA"
     gene            15758..16189
                     /locus_tag="PLM90_06305"
     CDS             15758..16189
                     /locus_tag="PLM90_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ89017.1"
                     /translation="MLSVSCKAAIKAVVFLGVQHQSSERYSIGEIAAEINENSHTVGK
                     LLQKLTHAGIINSIKGPHGGFYISEQQRHKHVIDIVEAIDGKEVFLECGLGFNKCSDS
                     KPCPFHNDFKKVRDLFTEMCRKKKICELDESVIRGNAYLRG"
     gene            16287..>16891
                     /locus_tag="PLM90_06310"
     CDS             16287..>16891
                     /locus_tag="PLM90_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HMZ89018.1"
                     /translation="MKHFSTRKLPILAQYAICIGVVILIAAICFSFTGIVGYRVIALV
                     LLMVVSFLAMLFEVYPVLVAAILSAVIWNFMFIPPIYTFHIDNTEDVLMFLLYFIIAS
                     VNAVLTIKIREAETKARDKEEKENTIKLYNTLLNSLSHELRTPISAILGAVDTLQEKD
                     SNLSEQNKSVLLSEIDTAGMRLNRQVDNLLNMSRLESGMLMP"
BASE COUNT         4929 a         3276 c         3240 g         5346 t
ORIGIN      
        1 ttttatactc aataccaagg tcgttccaat atccatagtc aagtggattt tctttagtca
       61 atcgttcaag tgttgatata tctgtcatta ctgtgtcgtc ttttaaaatg ggctataact
      121 tgtttattgc cgaagcatta tttccttcct aatatctata ccggagtata gccgaaggaa
      181 atgttaaaat ggtttcggct tatattagag gttgaggctg tcgatcggac tgctgatggc
      241 gttgatctga tgtgtgctga catgggtgta tatttctgtg gttttgatgt tcttatgtcc
      301 cagtaaaact tgtataaatc gcgtatcaac ccccgattcc agcatatgcg tggcgtatga
      361 atgtcgcagc cagtgcatgg taacaggttt ggtgatccca cttaaagtta ctgacttttt
      421 taataccaat tgaagactcc tttcggtata cttactgttg ggcttttccc cttcaaataa
      481 atagtatttt ggttggtacc tctctatata catatttaat ataggttcga cagatatggg
      541 taaaggcaca acccgatcct taaacccttt tgccagtcgg atctgtatac accttctcat
      601 gcggtcaata tcatcaaccc gcagggccaa aacttcccca acacgcaatc cgcacccata
      661 agccaaacgt aacatcagat tgtgtttgat gttatcagtg tgttttaata ttgataatac
      721 ctcttgtttc gacaatacgt ttggaagctt gtgttcccgt cgcggacgct ccagaaaatc
      781 agtgtttaca tgcaaaccat ataccatttg caaaaatagt ttcagcgcat ttacaaactg
      841 attctgatat gtgaacgata atttgcgagc aatgatatgc tccctaatga atgaaataaa
      901 atcctcccgg ttaatggcat gcggatcctt gtctctgtta accgagaaat ataatcgcac
      961 acaatcaaga tacactttta ttgtgcgttc gctataatgc ttgtgttgca tccactcctt
     1021 taatttttcc agttccgcta agcgcgcatc aggtaatggt tctgcaaccg aaacccggtt
     1081 ctttttttca accggttttg ctataccact attgtgcttt attgcttttg tatctaacca
     1141 cgcaacgcct ttaaatactg tgaataactt atgcagattt tcttttgtgg caggcaaata
     1201 ccaacattta ttttggaagc tgaaccggca acccgctttt tttgcctggt caacaagttt
     1261 tttatcataa gaaaaaaata atgcgtaatg ttctccacca cgatgcgtaa gcggtttgat
     1321 gataatattt gatagcatga aaactttagt tacccataga agttacccca aaatctccgt
     1381 tctgccttga acgttgctgt gtattgacta actgcccgcc ggtttcattg acttgttttg
     1441 tgttggaaaa ggtattttct gctaaaatcc cgtgcaatca atcaaccttt gatgccctaa
     1501 aaactcaata aactcctctt ctgaccctat ctttgccgca aaccacttaa agtgaaggga
     1561 atcattctag ccggcggatc cggcacaagg cttcatccaa ttactttggc tatcagcaaa
     1621 cagcttatgc ccatatacga taagcccatg atctactacc ccctgtcgac cctcatgatg
     1681 gcgggcatca aggatattct gatcatcagc accccttacg acctgccggg cttccaaaga
     1741 ttgctgggcg acggatctcg aataggatgc tcattttctt atgtagaaca accttccccc
     1801 gatgggcttg cacaggcatt taccctcgga gccgatttta tagggaacga taaggctgcc
     1861 ctggtgcttg gtgataatat cttttatggc agcggactgg gcgagctcct tactgctaac
     1921 aacaaccccg atggcggaat tatttatgcc taccatgtta gcgaccccga acgttatggc
     1981 gttgttgatt tcgatgcagc aggcaatgtg ctgtccattg aagaaaaacc acttcagcct
     2041 aaatcaaatt atgctgttcc cggattgtat ttctacgata ataccgtggt tgatatcgca
     2101 agaaacctta aacctagtcc gcgtggcgaa tatgaaatta ctgatgtaaa taaagaatat
     2161 ctccgtcaag gaaaattgaa ggtaagtgtg ctgccaagag gcactgcatg gttcgataca
     2221 ggcaccttcg actcattgat ggctgccagt actttcatcc aattaattga acaacgacaa
     2281 ggacaaaata tcggatgtat tgaggaaatt gcctatcgca agaaatttat taacgccgaa
     2341 caacttgaaa aaatagcaca accgttaatg aaaagtggct atgggcaata tttaatgaga
     2401 atcttgaagt aattagcaaa ttagctgatt agcaaattag caaatttaaa agttggcaag
     2461 ttagcaggtt agcaagttag caggtttgaa agttggcaag ttagcaagtt agcaagttag
     2521 caggtttgaa agttggcaag ttagcaagtt tgcaagtttg caggttagca agttagtagg
     2581 tttgaaagtt ggcaaacaaa caaataaatt agtaaatcag tacaacagaa aataattgaa
     2641 ttccatcggc taattggcta attaaaaaaa ttccatcggc taattggcta atttgctaat
     2701 tggctaatta aaaaaaaatt ccatcagcta attagctaat ttgctaattg gctaattaaa
     2761 aaaattccat cagctaatta gctaatttgc taatcagcta attaaacaaa ttctattgaa
     2821 tactattctc atcaccggcg gttgcggata cattggctct catacggcta ttgacctttt
     2881 ggaaaagggg tttaacgtta tttctataga taatctttcg cgctcaaaga cttttattgc
     2941 cgatagaatt cgagaaatca gcgggaagca atttcacaat tatcgcgtcg attgccgtga
     3001 tctggtgaaa atgcgaaata ttattgatca gcatggaaaa attgacggca tcattcattt
     3061 cgctgcattt aaatcggtag gcgaatctgt tgttaagcca ttggtatatt atgaaaataa
     3121 tatcaacagc acactcaata tgttgcgtat tgctgatgaa tttcatattc ccaatttcgt
     3181 attttcttca tcttgcagtg tttatggaaa taccgcgcaa caaccggtta aagaagacgc
     3241 accaatggcc acacctgaat cgccttacgg gtggactaaa ctcatcagcg aacaaatgat
     3301 tgctgcacat gctccaaatg tgcaaacaaa atttattgcg cttcgatatt ttaatccggt
     3361 gggcgcgcat catggtggga ggattggcga aattccttac agtgaacctg aaaatctggt
     3421 gccgaatatc acccaaacag caattggtaa gaaaaaagga tttaccattt tcggaaccga
     3481 ttatcctacc cgcgacggaa gctgcattcg cgattatgta cacgtgatgg atattgccgc
     3541 agcacatacc gctgcattgc aatatcaact ttccggacag caacaagttc ctttcgacgt
     3601 atttaacttg ggctcgggac gaggcgttac cgtgcttgaa cttattcgcg catttgaacg
     3661 tagtacaggg gtttcattgc catttgaaat tggtccgcgc cgtcagggtg atgtagttgc
     3721 aatttatgca aataccgaaa aagcaatgca atcacttcat tggcatccta cccgctctat
     3781 tgaggaaatg atgcattctg cctggacgtg ggaacaaaca atgcagaggg aatctattta
     3841 aattacaaac catgctgcaa accattttat tcgatttcca ggcattcttc ggacactcgc
     3901 caactgaggc ggctatcatc gtgttcaatc ttatcttgat tgaaagcatg ctcagtgtag
     3961 acaatgcgct ggtgatggga acaatggtaa tggacctccc aaaggaacag cgtggcaaag
     4021 ctttgcgata tggcattttt ggcgcatact tttttagagg cctggcatta ttgcttgcta
     4081 ctcaattaat taaggttgtt tggctgaagg cactgggcgg attatatctg ctataccttg
     4141 ctctggacta ttttcgaagc agcgcaacga atgatatttc ggacgatacg cttgacaaga
     4201 aatcaaattg gttttataaa aatactgtcg gaagaatcgg taacttctgg agtacggtta
     4261 ttctggtaaa tatcatggac ctggcattct ccatcgataa tgtgtttgct gccgttgcct
     4321 atacagacaa tatttatttg atctgtacgg gtgtttttat aggcatactt gccatacgtt
     4381 ttgttgctca aggcgtaata aaattgctgg agcgatttag ctttctgaat acctccgcat
     4441 ttctcatcat tggattgcta gggctgaaat tattagcttc atttatttgt aatgaatatg
     4501 gcagagaaac cccattctgt ttatttcttg aaagtgaaaa aacagacctt tatttttcat
     4561 tgattacagt ttgtattttt attttgccgg ttattacaag ttggcttttt aactttccga
     4621 agcgaagtca tgatcatcat acagatgagt ttgtgcactt tcaggaagaa gccgatgagg
     4681 ccgaacaccc caagcattca taactatgac caaaggtaga ctcgaagcat ttagcgatgg
     4741 tgtaatcgcc atcattataa ccattatggt gttggaattg aaagtgccgc acagcgcaga
     4801 gtggagcgca ctgcatgaac tgattgcggt atttatgagc tatgtgttga gttttgttta
     4861 tgtggcagtg tattggggca atcaccatca tctgttgcat actgtaaaaa atgtttctgc
     4921 cggtatcatc tggtctaatg cggcactttt atttttcttg tcgctgatac cattcgccac
     4981 agcatggatg ggtgaaaatc attttgcaca aaatacagtg atcgcatact ccatattgat
     5041 gaatttatgt ggaatgagct attatgtatt gatgtatatg attatgcaat gccacagcga
     5101 aaatgctgaa ttaacagcgg ccttgaatac gcagcgcaga aaaggaatac tgagtttatt
     5161 tctttactca ttctcaatcg gagcggcatt tatcaaccca atgattagcc aggtgatttt
     5221 tgttttggtt gcaattatgt ggtttatccc cgacaagaat attaaacgct tgatgcgata
     5281 atctgcacta tattttctgc taaattaggc catccataat gtatttattg aaataagatg
     5341 gatgcttcat ttaattaatg catattaaaa attccacttt ttagtataaa aatatatcat
     5401 attttagtcc attggtttag ctggcataat aattggtagt agatgctgtc ctttggtcat
     5461 atgaagtctt tgttatatac atttctgctc acttgtgcct tctttaccct ttcagcacaa
     5521 cctcaagaaa aggatggggg cttgtttatt caaaaaattg tttatttgga taatgtgttt
     5581 caaataaata atgcaggtgt tcttaagccg gtttcaggga catcaactaa tattcagcca
     5641 ctaatcattc agcatttcca attaagggaa gaagggaatc aacataaaaa acaacttcca
     5701 ttgtttaatc catccgattt tgattattat ggattgtatg ttcccgagtc ttttgcttat
     5761 tttatttctc ctagcatagg aaataatccg tctcctgacc aacgtatgat cattatagca
     5821 ggagcagatt ctatggtcat cgactttcat aatattccaa aaacgacacg ttatgaatta
     5881 actattttgc aacaaattta ttttatgccg ggatattata gtttggattg ttctttaccg
     5941 gcaagagatc ttaaaataaa tgaaaatgaa cgggaaatac tttatgctgc tgtaaaacat
     6001 ggaattacac cggaaacaaa atcaatactt agtaaagcag ggttgcttga aatggcacta
     6061 ccaacagaca ttgtaaaata tcagaaatat attccggccg ctataaccgt tgagcaatct
     6121 gatgcctata atataaatgt ttattttaca ggaaatttgc ttacaacaag taatggtatc
     6181 cttattactg actttcaaga atcctaccgg gggcattggc attctacagc atttagcaca
     6241 acgtttcatt acattggcga agaatccggc acctctgcgg tattttatga aaatcaaaag
     6301 agagtaatat ctcaaacaaa cccaatgggt gaacgcaatt taacagacgg atggtataga
     6361 ataggggttg tcaatagcta tggagattcg atttattcta aacccttctg tgtaggatta
     6421 gttgcggcaa atagcgatag tgtaacaaca ttatataata agcaacgtgg ttttccattt
     6481 cataatttca aagatgcaaa taccatcaac atcgagactg ctgttgcgcc tgtaataatt
     6541 gaccatcagc ccaaatatgt gttcaagtat cacattgtag tatgcaacct atgcctcttt
     6601 tctgatgagt tgtatggccg atatgatcaa ctcgccgcag aagagtttat gcacagatta
     6661 gcattcaacg atttgtatat taatggacaa ccatttaccg gcagcattga aatacaattc
     6721 ccgattaaag gggctactgt agaaaacgga ggtgcatggt tttatactaa tttaattgtt
     6781 acaaacacct atataaatgg aaaattacaa aaacattcgg agcgatgagg tgggatctga
     6841 gtaggaagtt ctgagcatat atgcgttaga cgaaaatgag tgtacgttat gcagtgttgg
     6901 gttttgcatg gctaagtatt tatttaagct agcgtgttta tccttttagt ttaacaatga
     6961 gatacctgca aaatgttttt ttccggcaaa caggtaaatt acgaaaataa tgctttgcaa
     7021 tgcattttta ttcttattat taaccctaaa ctagagccgt atattgatcc ttatccttgg
     7081 ccaaattgtt aattggccct tattttcggc tcttctcaaa atattttatg atttattatg
     7141 gaatttatat tgaacttgca tcttaacttt gaaaactaga tttatatttt atgcgtaacc
     7201 tattgatatt cggcactact atggttttaa tgcactgcgc tctccatgca caggattcca
     7261 gtcggatagt aattaacaca attaatatca atgattatac catagaattg gtcaaggttc
     7321 aacaaataga cgaagttgca gttaaaaggc ttaattatca agctccctat tcttggttat
     7381 atcaaattta tgaaaaggat tcattcataa ttgcggttca atactttaaa gattcatgtg
     7441 aactttattt tgattcccga ataaaaaagc aaggatttta ttatacgaca catggcgtaa
     7501 agattaatat ttgtgataag acagttacgc aaatcatgcc tcctgacgtc agttggatta
     7561 aagacacttt tgactcaatt accatatatc cgctcgattc agtgctagat ccttacgatt
     7621 atcaaggtca acgaacagta atttcaaatt attccgaaag agagcctgag catttaaacc
     7681 ttcaacatca aaaagatgtg gaatttatgt atcgggatcc aagagttata gggatgtatc
     7741 agaaaggctc agagttaaga ttacattccg cattaacgta ttataaatcc ggaaacgcac
     7801 agacaatata tttatattat gataagttta tgtacgaagg ctggttttac aacgtcggtt
     7861 attccgaaca tggaacatat aatgtagcga aaatggtgtg ggataagcac gaggcggcga
     7921 aataataata catacggaaa atatgtattt ccgtatcata tttacatttt gttgtatcct
     7981 attagcgaag cttttgcgac ttctgatagc tgattccacg ttcacccaga tggcgcatga
     8041 taaagtggaa atgtgcttct tcctctccta aaaactccgg cgtagaaatt ccttttcttg
     8101 tatacatctc ttccaatatt aatcgcgcag ccgcagtgca tgtatatccc gttgtgcgcg
     8161 ccatggaaga agttttagta gcaggatcat atctgtcgag cagatcatag gtataagtaa
     8221 cctggttatc attttcaatt ccgccaacga tcacacgcat taccgtaaat tcatgttctt
     8281 catcgcctag cttccatttg gggaataata attttgatgt tacatcaata gggcgaacat
     8341 gagtgccttt cacatcaata aactctttgt tgaaaaatcc tgtttcgcgc aatactttca
     8401 tcaatgcaat atgaccggga taacgcaacg tgcgttctat catatttgga atatgaggca
     8461 tggttttgat aagtgagcgc aagccatctg aattaaatgc ttccagtgtg ccgatgcctg
     8521 tgaaatccat atattcagga tcagtaagag cttctttgac aacaactttt ccatcctgaa
     8581 caaatctggc aggacgtgta tattcttcga tcacatcaat tggtgaaaac ggtgctttgt
     8641 attcatacgg ccataatcta gcagcaggta agccaccaac caaacaaaca aaatctgtca
     8701 cctgcatgcg tttgttgtga taacccagaa tgatattatc cattcccggc gccacaccaa
     8761 catccattac cgcagtaacc ccattttgtt tcgctaatgc atcaagtaaa aaaggatctt
     8821 catcaaaaaa agatatatcg ctggcatttt tacccgattc aattacagtt ttcaatgtct
     8881 caaatcccat aaaacccggc acagccccaa tcacaaggtc cttatcttca ataagtgatt
     8941 gtatcaattc cttgctggtc agatccgcat gaatggtgcg cacaccatgc ttttcaaggc
     9001 gttgaagcgc ttcggtattg atatcggcag aatacacttc ataatccttg gccagatcaa
     9061 tagccatagc tttccccacc attccggcac caagtacgat cacttttttt cttgacatag
     9121 caaattattt gccgtaaaga taggttaaaa cgtaaattgc tttgtggggc gatggggttt
     9181 atatcgtgta ttatgcagca cacttacata ctttcacact tacacactgc atttctgttc
     9241 ttgtgtgaaa gttgcacaat acggctgaag ttgtctgaat cacggattag acggattaaa
     9301 ggatttcgcg gatttaggat ttcatggatt tggaaatcta tgagagcgtt tgttgagtat
     9361 ggttgatgaa aaatgagata gactagtgtc aatcatagca ctaagcgctt aaaggttaaa
     9421 cttttactgc cgaagatgtc tgaatcacgg attnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnncaattgt tctattcata acactaagcg cttaaaggtt aaacttttac
     9601 tgccgaagat gtctgaatca cggattagac ggattaatgg atttcacgga ttaatgcatt
     9661 acatggattt gtttcgattg ttgtagagtg aggttttcgg tatttgatat tcaattgttc
     9721 tattcataac actaagcgct taaaggttaa acttttactg ccgaagttga tgagtagtcc
     9781 tacttctagt ttgtaggcct taaggtagtt tagggcttga gctagatgca catcttctag
     9841 ctgaataatt gctttgagtt caaccaagat tttgccctca accacaaaat cagccctgcg
     9901 tgtgcctatg ggaacttcca attccttgta ataaatatcc tgttcaatct ctctggcaaa
     9961 ttccaatccc gctttgcgca tttcatacgc taaagccctt tggtaaatga cctcttgaaa
    10021 gccgttgccc aagaatttat gcacttcaaa cgaagcacca atgattttct ccgaaatgtc
    10081 cttatacctc aattctccaa tcatcatgca aataaaattt aaatataatt tatttactaa
    10141 ataatttcat attcaatata aatctgcaag tatacataca attagtctta aaaaatccgt
    10201 ctaatcttta aatctgtgta atctctaaat ccttgtaatc cgtgattcag acaataatag
    10261 cccattattt ttgtgttcgt gtgttcatgt gcccatgtgt tcttgagcgc aagcaggagt
    10321 gttttttcgc tccggttttc aagtgagata cttagaagcc ggccattgct tttaaagatg
    10381 tctgaatcac ggattggacg gattaatgga tttcacggat ttaagaattt ataggggagc
    10441 ggtctaagca ttcagacctt cgcgcactaa tgaattattc aaatgatgtt aatgtgcaat
    10501 tttttgcatg tcttctcaat aaaaataatt ttcattagaa atccacctct aattagttaa
    10561 aaaaatccgt ctaatctcta aatctgtata atctctaaat ctgtataatc tttaaatctg
    10621 cataatcttt aaatctgtgt aatccgtgat tcagacaata attgcacatt atttattttg
    10681 ttcgtgtgtt catatgcgca tgtgatcttg agcgcaagca ggagtgtttt ttcgctccgg
    10741 ttttcaagtg agatacttag aagccggcca ttgcttttaa agatgtctga atcacggatt
    10801 ggacggatta atggatttca cggatttaag aatttatagg ggagcggtct aagcattcag
    10861 accttcgcgc actaatgaat tattcaaatg atgttaatgt gcaatttttt gcatgtcttc
    10921 tcaataaaaa taattttcat tagaaatcca cctctaatta gttaaaaaaa tccgtctaat
    10981 ctctaaatct gtataatctt taaatctgtg taatctctaa atctgtgtaa tccgtgattc
    11041 agacaacaat tgcacacgga ttttttaatc agactctaat ttaatttagt gatgccatac
    11101 attacttcct ataacttgac gtgcttaaac cttcccgacc tagcgaattt cactatcgaa
    11161 ttgacaaatt tgttagttgc catcgcccta tatttccaca ccttccggtc cttctcgcga
    11221 attcccgtaa tctctccata ttgtatttgt aaaccatttt tactaatttt atataaaatt
    11281 caatcacatg aacacattcg accgcatggg caatggctgg aaaatctcga tggcagggtt
    11341 ttcggtaatt cgcaaaaaca aacaattatt agtttttcca atcctttcag gagcggtaat
    11401 gttattgatc atcgccactt ttatattcgc aatgttgggt tccgatttcg ggcaagtaca
    11461 acacctgctc gacaatggta atccaatgcg atattttatt ttgtttgttt attacctcat
    11521 tagctatttt gtagttgtgt tcttcaacat ggcattaatt cactgtgccc gcctttattt
    11581 tcaaggtaag acaccaacaa tttccgatgg acttaatttc agcatgagcc gcataggggt
    11641 gattttttct tgggccttgg tagcggcgac ggttggtttg attttgcgat caattcagga
    11701 aaaggtaggc atagtaggta agataattac cggtcttgtt ggccttgtat ggagtgttgc
    11761 tacatttttt gtggtcccgg tactggcata tgaaaatttg cgccctattg atgcagtaaa
    11821 gcgttctgct caaattatgc gcgaaaaatg gggtgaaagc attggtgccg gttttagttt
    11881 acttgtattt aatcttctcg gcttattatt ggtagctgta cctttattct ttatcggctc
    11941 gctaataagc acacttgctt ccatgatact cgcttttctt ggtgttctaa ttgttaccac
    12001 aataagtagc gctgcacaaa caatttttat cagcgccgct tatttaaaaa tcaacaatga
    12061 agagatagaa ggctttgatg atgatacgat tgacggattg ttttaccgca aggactaatg
    12121 gaaacaccaa ttcaatctat tcctccaata gatcgttttg aacccggctc gttttccaca
    12181 acgaagtcaa ggccctttcc accgcatgtt tttgtggtgg tctttattat tgtagttgga
    12241 atgttcctca atatgcctag cccgataccg gcgcttttac ttgcaatggt agatttcgga
    12301 ttattatcta tgcgatcggg tgtagaggtg ttgattaatg caaaatcata cagagatttt
    12361 accagcatct atgggattaa attcggtaaa tggaaatctt taggtgaata tgatttcata
    12421 ctgattaccc gcattaataa acgtagaaac gcaatgatga atatagcgta tggcgtttct
    12481 tatggtgatg ttttcttcga tatggatttg attacaaaag gtgatagaga cgtgttgtta
    12541 aatcgtttcg gcgaaagaga aattgtaatt gataaggcaa tccaaattgg tcaatatttc
    12601 gatttgcgcg ttgtggaaag agtggatgga gattatcagg tagtttttga accggaaaag
    12661 aaggtggtca aataatgcaa aattttatga cagctgaaac aaaacgcagt ttaatttttt
    12721 ctgttctgcc aaaattcgca tacactatat attgccatca ctttttattt ctttctctcc
    12781 atctcagaaa tactgtttcc taaagttcct ttgattaacc ctcggcaatt cttgctaccg
    12841 caatggcatt caaacggttc catgtgctca tctaaaaatg tagcgtaatc aagtgtaagt
    12901 tcttcaccac atgaaatatt gcgcagtgct acaacatcca aaccatcgta tgccgtagat
    12961 ggctcgcagc tgtgattttg tggcgcccag gcattggggt cattatccca caataaaaac
    13021 acttcattgc ttatcggata ggcatatctc ctaaaaacta attgcatgtc ttcaggccaa
    13081 tgtgtttcta cgtgtcgccg tgtggtgatg cgctgtgatg cttcttcacc atgaaaaatc
    13141 acttcgccct tctcaatatc acgtgtcgca taaataccat atcctgtgcg cgcatttcct
    13201 tttaaacaaa atggtttatg tgttcgacga taacgcgcca tcccttcatc aataatagca
    13261 cgtaaaaaac cttcctgtcc aataccgtct tcgcgcaaaa tataatctgc tgatccctca
    13321 tatccgtcgc tataaaaaac ggaacaggtg aaattgatct ccaaaaaata aaggtcgcct
    13381 ttatcatcca ttctgaaatc taatcgggcg taaccaactc cgttgaagcc tgtaaatatt
    13441 aagcgagtgg cgtcttgcag tttttttatg atatccgcat ccgttacagg aatgttggca
    13501 ttgggatgta attctgatgt tttaagggca taggttttaa aagcaaagcc ttcaggaaaa
    13561 atatactcta tgggctgcaa cacgcgagct gcaccatttt catcgggtga ggctattacc
    13621 agtgctgtaa actctctgcc ggcaatatat tcttccacca acaaaggacc gtattcttca
    13681 ataattgatt cggccttttt ctttaatgct gttacatcat gcaccagtga cttttcatca
    13741 acccccaaac tatctcctgc ttttgatggt tttacaaata aaggaaactg aagttgtgat
    13801 gcaatggaat ctatctcatc aaggttttgt aataatacaa atgctggcgt ttttacaccg
    13861 catgtaaatg ctacatattt cattaactcc ttggtaggat cgtagagtaa tgatgaaggt
    13921 ccggtaaaag gcaagcccag caattccatg caataaatca catcaattga cggaatactc
    13981 caatctaaat aaccttcaca caggtttaca taaatatcgt agcccttttt cttaagttca
    14041 cgtaactgct tgtaggtagt aagcttattt aaaaaaacgt gatctacaat tgcatttggc
    14101 aatagcggtt gaagattgcg agggggatcg tagtattgat aatctacatc gctggttgaa
    14161 taatccggtt gcaaaacaca tactttgaat ggcgctacac tgctcatgaa attcaaccga
    14221 tttataatga tgcttattta ttgatttttt tatgcttcgc gatatgactt ctgcaatttt
    14281 ctaacgcgat tctttttcat ccaacgatat gtggcatcat tgatgatctc aactaccatt
    14341 tgagcaaatg atttattact catgcgcaaa atcgcaccaa tggaagtata atcttcatcc
    14401 tcacttaaac cgcattgtgc gttcacttcc agcacgccaa tttcaccggt atgcttgtcc
    14461 atgcgcaggt ctacccgcgt ataacccttg ccgccaaccg atacataggc atcccaactg
    14521 atgcgtttca atttactgat gagcgaacgt gccggtaagg tgtaattata gaaattatcg
    14581 ttttccggca tgggtgtttc gtcttcatat atttcccata gcctgtcgaa agagagaaat
    14641 ttttccttat ctgggagcga tggatgaaat acgcgttcaa ccggtgtata aatcacacaa
    14701 tttcgtggtt ggtcagaagc gccaatgatc atggtggtgt attccggccc attgatatat
    14761 tgctcaacaa caatgcctcc ggccgataat tgccatccgc gatagccatc aaatatttta
    14821 ttgatttgct ctttcaactc tgtttcattc tctaccacat tttttacatt caatcccatg
    14881 ctcccgccag aaactgctgg ctttacgatc agcggagatc ctaatcgctg aaatatgccg
    14941 ctgatgtcct gattgcgatc tgttaccgct tcccatttgg cagtaggcac tcctgcaaga
    15001 tcaaacgcct ccttcatggt gattttggaa gtcgtaatat cgtagaaata tgcatcggcg
    15061 ccggtataaa tgagtttgtg cttttctaat gcatgcacca ccgatacgcc cggtgcacca
    15121 tttacttcat cgccatcaca aatattaaac actaccggaa atgataaacc cgcatttctc
    15181 tccgctgcaa tatcggcaat cacattttgg taattaccca tattcaccgg ctgccattgc
    15241 cattgaattt gcaactgtct aaaagtcttc gtgtattctt ctatactttg ggtgaaatca
    15301 taatagtaat ccaggttagg atctgatgtt tcaagttgcg gtgccaaaac ccaaactttt
    15361 agttggtcta agggtagggc atcatgtagt aaaaatcggg ggtcggcggt cggggccaac
    15421 tgttgctcct gctccatgca gtggggtaat gttttggttg atataaagat acattaagta
    15481 tgtatccatg caagattatt gaggtggata atgaaaaata tccgactact aaatttggat
    15541 ggcttgctta cctttgccgt tggcttttgg actataacct gattttttgc caaacttggc
    15601 tatgtgttag tataattgtt taattatcaa tgcattataa agcgatttgc aaaaataaag
    15661 taagcgaata ctttaattat actacaatcc atatctttgg tttaaataat gtatcgggtg
    15721 cttctaaggt cacaaacatc ttataaaagt ttatgcaatg ctgagtgtat catgtaaggc
    15781 agcaattaaa gcagtagtat ttctgggtgt tcaacatcaa tctagcgaac gatacagtat
    15841 tggagaaatt gctgcggaaa taaatgagaa tagtcatacc gtaggcaagc tgttgcaaaa
    15901 actcacgcat gccggcatca tcaacagcat caaaggtccg cacggaggat tttatatcag
    15961 cgaacaacaa aggcataaac acgttatcga tatagtagaa gctattgatg gaaaggaagt
    16021 ttttttagaa tgcggattag gatttaataa atgttcagat tcgaaacctt gtccttttca
    16081 taacgacttc aagaaagtgc gtgatctgtt cacagaaatg tgtcgcaaga aaaaaatatg
    16141 tgaactcgac gaatcggtaa ttcgaggaaa tgcttatttg aggggataac atgtttaatg
    16201 tgtagatcac agttgcttct attctgcaaa agattttata ttatctttat agtgcatacc
    16261 atacttttga tcgattctgc acatcgatga agcatttcag cacacgaaaa cttcctatcc
    16321 ttgcacaata tgccatttgc ataggtgtgg tgattctcat tgccgcaatt tgtttcagct
    16381 ttacaggtat tgtcggctat cgggtaattg cactggtttt gctcatggtg gtgagcttcc
    16441 tggccatgtt gttcgaagta tatcctgtgc tggtagccgc aattctgagt gcagttatat
    16501 ggaacttcat gttcattcca cctatttaca catttcacat cgacaataca gaagatgtgt
    16561 tgatgtttct gttatatttt atcatcgcct ccgtaaatgc agtgcttacc atcaaaatca
    16621 gggaagctga aacaaaggca cgtgataagg aagaaaaaga aaataccatc aagctttata
    16681 acacattatt aaattcttta tctcatgaat tgcgcacgcc aatttcggca atattagggg
    16741 cagtagatac cttacaggaa aaagattcaa atctcagtga acaaaataaa tctgttttat
    16801 tgagtgaaat agatactgca ggcatgcgct taaataggca ggtagataat ttattaaaca
    16861 tgagccgact ggaaagtggt atgctcatgc c
//
LOCUS       DAOQOU010000051        16784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1716_length_16784_cov_6.267559, whole genome shotgun sequence.
ACCESSION   DAOQOU010000051 DAOQOU010000000
VERSION     DAOQOU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16784
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_16784_cov_6.267559"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLM90_06315"
     CDS             complement(<1..201)
                     /locus_tag="PLM90_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMZ89019.1"
                     /translation="MKRVVAFWICCLCASVTYAQDIHFSQFFAAPILVNPANTGNFIG
                     SARIGLNYRDQWGSVTIPYRTFS"
     gene            complement(198..3734)
                     /locus_tag="PLM90_06320"
     CDS             complement(198..3734)
                     /locus_tag="PLM90_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ89020.1"
                     /translation="MKSARYYIWLLIMTLLSATGNRNVCAQQVKVPHAQPIKFIENKS
                     QWPEQVHFKGEFQGNSVYLEPTAFTFDLVDSRDLMEARHAHLSPNEPTPPVPEIHRHA
                     YRMQFIDANEQVQFATGNKSKEYYSYLLGNDPSKWGHQAYAYNDVTYQSLYQGIDIKV
                     YTSGENMKYDFIVKPGAKVEDIQIQYDGLIEVIAADGNLYLVTSIDTIVEMQPYAYQK
                     IGGRTIAIKCNFVVNGNQVHFEVEGNYNKNYELIIDPELVFASYTGSSADNWGYTATY
                     DEDGNLFGGGIAFGTGYPITVGAYMTSYQGGTVDISISKFTSDGTDLIYSTYLGGNSS
                     ELPHSLIATVTGELIIYGTTGSADFPITAGAYDNTFNGGAGVTVDGIVNFSSGIDIYV
                     AKLSADGTNLLASTYIGGTSNDGMNLATGLSTQYNYGDFARGEVIIDDANNVYVASST
                     ISSNFPTTPGAFQTTLGGDQEGIVFKFNPSLTTLAWSSFIGGNQEDGAYSMKINSLGQ
                     PVVCGGTASSDFPVTAGSWDNTYSGGVTDGWVAKIAADGSAILDATFAGTNNYDQVFF
                     VETDEADNIYITGQTKGTWTVTPGVYTEAGGKQFITKLESDLSAVIYSMVFGSGGSAI
                     NISPSAFLVDDCENVYVSGWGGSVNQGYNFATGTTSGMTITPDAIQSTTDGDDFYFFI
                     VSKNAIDLLFASYFGGPSSPEHVDGGTSRFDKSGAIYQAVCAGCWGLDDFPTTAGAWS
                     ETNGSFLCNLGVARIDLNVGGIYASAAADPSFIGCAPFDVSFINTSSDAIDYIWDFGD
                     GSPVSNAFEPSHTYLDPGTYDVMLIVIDSNSCNLADTAYLEVQVLADSLSAEFEYTSV
                     ENCDSLIATFTTIGFLLPTTTFLWDFGDGTTSTLINPSHTYFDPGSYTVTLIVTDPFS
                     CNGVDTFSLTFNYLFEFNTGFVSEALGCLPVDATFTASFTGSDQYLWILGDGTIDSGE
                     VVTHTYTVAGTYEVSLIMLNCGIPDTVTQIVVIDGWPVAYFDDDPYYIIKNTTVTFTN
                     LSTNAVTYEWDFGDGGTSTEVNGKHIFTELGTYQVCLTATNSNGCSDVYCREVVAEAQ
                     GAIGVPNAFSPNNDGVNDVLFVMGFGIADMQFLVFNRWGELVFSTDDYQVGWDGTYRN
                     AQQEMEVYVYVLSGTFADGNPFNLQGNITLIR"
     gene            complement(3961..5799)
                     /gene="glmS"
                     /locus_tag="PLM90_06325"
     CDS             complement(3961..5799)
                     /gene="glmS"
                     /locus_tag="PLM90_06325"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMZ89021.1"
                     /translation="MCGIVGYIGHREAYPVLIKGLHRLEYRGYDSAGVALINGDLKVY
                     KIAGKVSELEKVCEDQNTQGTLGIGHTRWATHGEPNTRNAHPHISESGNLAIIHNGII
                     ENYAPLKTELSKRGHKFLSDTDTEVLIHLIEDIQINEKLDLVEAVRVALNQVVGAYAI
                     VIVSKDEPDMLIAARKSSPMVVGVGDNETFVASDASPLIEYTRNVVYLNDEEIAIVRR
                     NKELEIITIHNENITPYMQELQMQLDMIEKGGYDHYMLKEIYEQPNSIQDSMRGRLIG
                     SRNAIVMGGIKEYEKKLVNAKRIIIIGCGTSWHAGLVGEYLFEDLARIPTEVEYASEF
                     RYRNPVITEDDIVVAISQSGETADTLAAIELAKSKGATIFGICNVVGSSIPRATHAGS
                     YTHAGPEIGVASTKAFTAQVTVLTLIALAIARKRGTITESKYQSLTVELSKIPEKVKQ
                     VLEANDHIKQIAEEYQNVHNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDENMPVVVMATNMSAHDKIVSNIQEIRARKGRIIAIISENDNVIETMAEHVI
                     RIPETEEALTPLLSVVPLQLLAYHIAVMLGRNVDQPRNLAKSVTVE"
     gene            complement(5803..7155)
                     /locus_tag="PLM90_06330"
     CDS             complement(5803..7155)
                     /locus_tag="PLM90_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HMZ89022.1"
                     /translation="MNRALTLLMCGCIVILTSCDKDNFIGKDLLPDEDFLNSEQIDTF
                     KIITYTDFDDSVVTSQNVFYALGSLDNQRYGKATASIYSQVLLPANNLFFGDAPEVDS
                     VVLTLDYSGYYGDTTLYHSLNVYRMSEPMQSGRNYHSDDKFRHIPVPIGRKSNFKVNM
                     LDSVMLADSSLWEPHLRIPMYNTFGTNIINLDSTILASDTSFLEYLQGIYITPDTSAG
                     YSNAIMYFDMASDISGLRIYYHNSEADSLSVMLPLTGTKCNSFTNQLHPGTDIGDAIM
                     NADTTDGDAFTYVKGFAGARTIVKLPTLTNLQDVSINKAELVLTMTNDGSRYYPAPPK
                     VQLVQLDSTGRNYYYLALYTYELYSSIVDDNFGSTNIGGVAIKTTRDNTGRTVYEYQY
                     NITKHVQEIIEGSLDNNGFALICYPTNRVPNAVTLGGPMCPREDFKPRFTVTYTTINK
                     "
     gene            complement(7183..7992)
                     /locus_tag="PLM90_06335"
     CDS             complement(7183..7992)
                     /locus_tag="PLM90_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HMZ89023.1"
                     /translation="MEKKRVLIITQEMQPYHNGTDIGDIARELPQYVQDQGNEIRVLM
                     PRYGTINERRHRLHEVVRLSGMNIIINDDDYPLIIKVASVPGARMQVYFLDNDDLFKR
                     KHVFHDDNNKFFEDNAERMVFFCKAALETVKKFQWAPDIIHCHGWMTSLVPLYIKTTY
                     KNEPVFQNAKVVYSMYKGAFDEKLAKDKFLEKAPISTHIEADHLNEYKEYNHIGLSKG
                     GAKYADGVIIGSEDLEKELGKYAKSLGIPVLEYAKESYLEKYAAFYNELYQ"
     gene            8165..9061
                     /gene="panC"
                     /locus_tag="PLM90_06340"
     CDS             8165..9061
                     /gene="panC"
                     /locus_tag="PLM90_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HMZ89024.1"
                     /translation="MQVITTRSALTPLLLREKALGRKIGFVPTMGALHDGHLSLIAES
                     RKRADITVSSVFVNPTQFNDPKDLAKYPRQPEKDAAMLEKAGCDYLYLPAVEDVYPEG
                     PGKGPFFDLGGIDTRLEGASRPGHFAGVAEVVKVLVETLVPDFLFLGQKDFQQCMVVK
                     RMLDQWDKHHWDSALQEASLNWRPGDVELVICPIIREAHGLAMSSRNERLSGEMRTRA
                     SAIYASIQYAEELFPTFSADEISTICANALNSIPDFQVDYFQILDGETLSPANADTQK
                     FVIITAVIVAGVRLLDNKIIIN"
     gene            complement(9084..9848)
                     /locus_tag="PLM90_06345"
     CDS             complement(9084..9848)
                     /locus_tag="PLM90_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89025.1"
                     /translation="MEYPFEQRYKNFSIDELLEVIQFPDDYVPEAVMAAKAELSLRQL
                     TDEQLAEAQSVLDERKSQIRKKQELKQRQMEVVSDALDSIAREFIPGQTEPQRGDRLV
                     KIIGIICSIMVLAAFYQNRYLIQMVSRDDLMLVDAFASLLPLVIWIIAIIFFFMRKKA
                     GWVMIGIIIAATAGLMIGQLILSPLAPEPFIQYTDSGQSNATLLLGTILVLALNYGLI
                     RMEVREIFNVSTSLKNKIIITFSALGFLLAGVFILG"
     gene            complement(9973..10335)
                     /locus_tag="PLM90_06350"
     CDS             complement(9973..10335)
                     /locus_tag="PLM90_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ89026.1"
                     /translation="MRKLLAALSVCLILASCGGSGSKPSADAVKNLETAKRLEGKALL
                     TKNDCLTCHIEQEKLVGPAYKDVAAKYAGQEGAANMLVDKVINGGSGVWGDVAMAAHP
                     NLSRSDAEKMVNYILTVK"
     gene            complement(10443..11378)
                     /locus_tag="PLM90_06355"
     CDS             complement(10443..11378)
                     /locus_tag="PLM90_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89027.1"
                     /translation="MKTLCFLVAMGMLISLSAQTPLNKASECFDLYDAKQYQAALKCA
                     DGKIKNDSMLVEAYIAKGMALIELKKYQEAYDAYSLGIQRTGVKNELINHRGVFLLSL
                     QEFDAAIKDLSIAYEYAPDDTLKRFALVNRAAAYMSIRQFEMAYDDLLAAVKLDSSDV
                     SAYINLGVIADEIGKGELTLKYYEKALSLDSTQMMVYVNIGFKYQLDGQHDKAIKCFN
                     KVLEADPNDGVAYNNRGYSKMMTGDLKGAMEDVNKAIKLYPANSYAYRNRALIYLRMD
                     KQDKACDDITEALNKGFTTMYGDEMEKLYNKNCLK"
     gene            complement(11445..13652)
                     /locus_tag="PLM90_06360"
     CDS             complement(11445..13652)
                     /locus_tag="PLM90_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HMZ89028.1"
                     /translation="MRAFVFFLLIISNCSKAFTGVVHYDLHITDTTLYFNGKSVRAIA
                     VNGQVPMPTLVFTEGDTAEISVFNHLTESTSLHWHGVFLPNAEDGVPFLTQLPIEPGA
                     TYTYRFPVIQNGTHWYHSHSGLQEQIGMYGMLIFNKRSDDAQIRTGIDDIQNQEIILS
                     EWTTMHPNYIHHLLRGGSDWFTIKKHAAQSYAEAIGNHALGVKLSNEWKRMNAMDVSD
                     VFYDDILVNGQTSFQLLPADSLLRLRIANGSASTYYWLSYAGGKVMVIANDGNDVEPV
                     EVDRMLIAVSETYDIIVKIPTGHTYEFLVMSEDRSQSASIWLGSGDTIFAYKWPKLSY
                     FEGMKMMNGMMRMNGTMDAMGMRMGMQVMDMNEVMYPELNQQENVAIDHIHEVDEMQN
                     THTIGFPQTLNYSMLKATTNTAITGYDSIKTLHFTLTGNMQRYVWSINGKVVSEADKI
                     LIHKNELVRIVLTNNSMMRHPMHLHGHDFRILNGQGNYAPLKNVLDIMPMETDTIEFL
                     ANADGDWYFHCHLLYHMMSGMGRVFTIEDDTDTLRFIQNAYSRRKFYNEDKALHFMAD
                     GFLANNGSHGMAMIQNTRWQFSADWMVPFNPHPTYESEIHLGRFIGSNQWFMPYIGLM
                     YKSGRFGHDETMENIFGQNIIDRDPLLPAIGFTYLIPFNFTLQSDIMLDGSLRVQLMR
                     DDIPITPRLKLFVMGNTDMEYEVGLKYVIKPYLGLSVNYGNMMGAGAGLSFTY"
     gene            complement(13649..14131)
                     /locus_tag="PLM90_06365"
     CDS             complement(13649..14131)
                     /locus_tag="PLM90_06365"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HMZ89029.1"
                     /translation="MKSFILFSLIAIIHSISIAQGVSAGQILNRYMVVKEALSEGNYV
                     QANQAGALLKAALFVYDDSSETEKTAFLENVSEMYKYASKISSSSDLNEIRNAFAKLT
                     PAVWNFVSSSPAFNLNYNYLYCTMQKAYWIDYDSSVINPYVDKSMQECGVIRNLNVLQ
                     "
     gene            complement(14139..14375)
                     /locus_tag="PLM90_06370"
     CDS             complement(14139..14375)
                     /locus_tag="PLM90_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89030.1"
                     /translation="MENQIDKMVITNTFKTNINCSGCVARVKIELEKLTELSHWSVNT
                     MVPDKFLSVTAPEARMEEVISAVKRAGFNIEKIK"
     gene            complement(14450..16624)
                     /locus_tag="PLM90_06375"
     CDS             complement(14450..16624)
                     /locus_tag="PLM90_06375"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ89031.1"
                     /translation="MTCASCAISVESIVKAQPGVIKSVVNFADASLLVEYIPTIINPQ
                     QIRAAVQSIGYDLLIVDANVEDTLEQIQQKNYLRLFNRTAGAVVLSIPLILISMVFMD
                     MPRANYLMWLLSTPVVLWFGRSFFVNAWKQAIHRKANMDTLVALSTGISYIFSVFNTV
                     NPAFWTSRGLDSHVYFETASVIIAFILLGKLLEERAKGKTASAIKKLIGLQESSVQVL
                     QKDGSVRNEQINNVQHDEIIVIKPGAKIPLDGIITAGESFVDERALTGEPIPVFKSKD
                     DKVFAGSLNGSAVLKVKVTHKGSETMLAQIISMVRNAQGSKAPVQKLVDKIAAIFVPI
                     VMLLALLSGLLWWVLGGENGLTQGLLAMTTVLVIACPCALGLATPTAIMVGVGRGAAN
                     GILIKDAASLEQARDITAVVLDKTGTITRGEPKVSEIIWFDDNPIYKQLLFTIEKYSS
                     HPLANAIVQSMPESSILALETSTAVGGKGMIAKYQEKNWFVGNSKLIQENKVSISDTL
                     MVKANELVSQGSSIVWFTDENQVYALIAISDEIKSSSKMAISQLKEMGIEVHMLSGDN
                     QISAESIAHEVGITYVKAGVLPAEKATYIKKLQEAGKKVAMVGDGINDTAALAQSDVS
                     IAMGAGSDIALDVAKITLMSSDLLKVPAAIKLSVKTVRAIRQNLFWAFIYNIIGIPIA
                     AGILIPITGFQLNPMLAAAAMALSSISVVGNSLRLKWSHIGM"
BASE COUNT         4855 a         3671 c         3216 g         5042 t
ORIGIN      
        1 ggaaaaagtg cggtatggaa tggtaacact accccactga tcgcgataat ttaatccgat
       61 acgtgcagat ccgataaaat ttcctgtgtt tgcaggattt acaagaattg gtgcggcaaa
      121 aaattgagaa aaatgtatat cctgcgcata ggtgacactt gcacacaaac aacagatcca
      181 aaatgccact acacgtttca tctgatcaat gttatatttc cttgtagatt aaatggattt
      241 ccatctgcaa atgtgccgct aagcacatac acgtatactt ccatttcttg ttgcgcattt
      301 cgatatgtgc cgtcccatcc gacctgataa tcgtctgttg aaaatactaa ttcaccccat
      361 ctgttaaata ctaaaaattg catatcggca attccgaacc ccataacaaa caatacatca
      421 ttgacgccat cgttattcgg tgaaaatgcg tttggaacac ctatggcgcc ttgtgcttca
      481 gcgacaactt ctctgcaata aacatcagaa catccatttg aattagttgc tgtgaggcat
      541 acctgataag tacccaactc ggtaaaaata tgcttgccgt tcacttcggt tgaagttcct
      601 ccatcgccaa aatcccattc gtaagttacg gcgttggtgc tgagattagt gaatgtgaca
      661 gttgtatttt taatgatgta atacgggtcg tcatcgaaat atgcaacggg ccaaccatca
      721 attacaacta tttgtgttac ggtatccgga atgccgcaat taagcatgat cagagaaact
      781 tcataagtac cggcaacggt ataagtatgt gtaacaacct cccctgaatc gatggtgcca
      841 tcacccaata tccataaata ttgatcgctg ccggtaaagc ttgccgtaaa tgttgcatca
      901 acggggaggc aacccaatgc ttcgcttaca aagccggtat taaattcaaa taaataattg
      961 aacgtaagac taaatgtatc tacaccatta caactaaaag gatctgtaac aatcaaggtg
     1021 acagtataag aacccgggtc gaaataggta tgcgatggat tgatgagtgt tgatgtggtt
     1081 ccatcaccaa aatcccacag gaaagtagtt gtaggcaaca aaaatcctat tgttgtaaaa
     1141 gttgcaatca ggctatcgca attttcaacg gaagtatatt caaattccgc cgacaatgaa
     1201 tcggccagca cttgtacttc gagatatgcg gtatctgcga gattacacga attcgaatcg
     1261 ataacgatca acattacatc gtatgtaccg gggtcgaggt aagtgtgact tggctcgaaa
     1321 gcatttgaca ccggtgaacc gtcgccgaaa tcccagatat aatctatagc atcagaactt
     1381 gtattgatga atgaaacatc aaaaggagca caaccaataa aagatgggtc agcagctgcg
     1441 ctcgcatata tacctccaac atttaaatca attctggcaa cacccaggtt acataaaaag
     1501 cttccgttcg tttcgctcca tgcacctgca gttgttggaa agtcatctaa accccagcaa
     1561 ccggcgcaaa ctgcctgata aattgcaccg gatttatcga atctgcttgt gccaccatct
     1621 acgtgctcag ggctagatgg tccgccaaaa taggatgcaa ataatagatc gatggcattt
     1681 tttgaaacga taaagaaata aaaatcatcg ccatctgttg ttgactgaat agcatcaggg
     1741 gttattgtca ttcctgacgt ggtacctgtt gcaaaattat acccttgatt cacagaacca
     1801 ccccaacctg aaacatatac attctcacaa tcatcaacca aaaatgcgga aggagaaata
     1861 ttaattgctg agcctcctga accaaaaacc atagaataaa ttaccgcact taaatctgac
     1921 tccagtttgg tgatgaattg ttttccacct gcttcggtat acacgcccgg cgttacagtc
     1981 caggtacctt ttgtttgacc ggtgatataa atattatccg cttcatctgt ttcaacaaaa
     2041 aacacctgat cataattatt ggtgccggca aatgtagcat cgagaatggc gctgccatct
     2101 gcagcaattt tcgcaaccca accatctgta acaccacctg agtatgtgtt atcccacgaa
     2161 cctgcagtaa ccgggaaatc actacttgcg gttccgccgc ataccaccgg ttgaccaaga
     2221 ctattaattt tcattgaata agccccatct tcctggtttc cgccaataaa cgaactccat
     2281 gcaagggtgg ttagcgatgg attaaattta aatactatac cttcctgatc gccgcctaat
     2341 gttgtttgaa atgcgccggg cgttgtaggg aaatttgaac ttattgtact acttgctaca
     2401 tatacattgt tggcgtcgtc tataattact tcaccgcgcg caaaatctcc ataattatat
     2461 tgtgtgctca aacctgttgc cagattcatg ccatcatttg atgttccgcc aatgtatgtt
     2521 gatgccaata aatttgttcc atcggcactt agcttggcaa cataaatatc gatacctgaa
     2581 gaaaaattca cgatgccatc aactgttaca cctgcgccgc cattaaatgt attatcatat
     2641 gctccggcag tgattgggaa atcggcacta cctgtagtac cataaataat caattcaccg
     2701 gttacggttg caattaaact gtgtggcaac tcggaactat ttcctcctaa atatgtgcta
     2761 taaataaggt cagttccatc agatgtgaat ttcgatatac taatatcaac agttccaccc
     2821 tgataactgg tcatatatgc gcctacggtg attggatacc ctgttccgaa agctatacct
     2881 ccaccgaata aatttccatc ctcatcataa gtagcagtat atccccagtt gtcggcactt
     2941 gatcctgtgt aagaagcaaa tactaattcc gggtcaataa tcagttcgta attcttattg
     3001 taattccctt caacttcgaa atgcacctga ttgccattaa caacgaaatt gcatttaatt
     3061 gcaatagtgc ggccaccgat tttttgatat gcataaggtt gcatttcaac aatggtgtca
     3121 attgaagtta ccagatacag attcccatct gccgcaatta cctctattaa tccatcatac
     3181 tgaatttgaa tatcttcaac tttcgcaccg ggcttcacga tgaaatcata tttcatattt
     3241 tcacctgagg tatagacctt tatgtctatt ccctgataca gcgattgata ggttacatcg
     3301 ttatatgcat aagcctgatg tccccatttc gaaggatcgt ttcctaataa atacgagtaa
     3361 tattcttttg atttatttcc cgttgcaaac tgtacttgct cattcgcatc tataaattgc
     3421 atgcgataag cgtgtcgatg aatttcaggc acaggaggtg ttggttcatt aggactcaaa
     3481 tgggcatgac gtgcttccat caggtcgcgc gagtcaacaa ggtcgaaagt gaatgctgtt
     3541 ggctcgaggt aaacggaatt tccctgaaac tcgcctttaa agtgtacctg ttcaggccat
     3601 tgagatttgt tttcaataaa ttttatgggt tgtgcatgcg gcaccttgac ttgttgggca
     3661 cacacattcc tattgcctgt tgccgacagc aatgtcatga tgaggagcca tatgtagtaa
     3721 cgtgccgatt tcacgattat tcggtttgtt ccttgagcaa aaaaacgcca ttgcaacaga
     3781 ggttacaacg acagattttt gcgactttcg ccacaatacc actttaaaaa tactaaaaag
     3841 cgtcggatat tcttccgaac tcgtggctca atcctgccta aaaagcacct tttcagccat
     3901 aatagcacga aaaagcacta aaaatgagat gtttctgacc gaatttgggt tgtatgagac
     3961 ttattctacc gttacagatt tggcaaggtt acgcggttgg tcgacattgc ggccgagcat
     4021 tactgcgata tgataagcca acaattgcaa aggcacaacg ctgagtaaag gggtaagtgc
     4081 ctcttccgtt tcaggaatgc ggatcacgtg ttctgccatt gtttcgatta cattatcgtt
     4141 ttcagaaatg atggcaatga tgcgtccttt tcttgcgcgt atctcttgaa tattacttac
     4201 gattttgtca tgtgcactca tattggtagc cattaccaca accggcatgt tctcatcgat
     4261 gagtgcgatg ggaccatgtt tcatttccgc agcgggataa ccttcagcat gaatatatga
     4321 tatctcttta agtttcaacg caccttccag cgccaccggg aaattatatc cgcgaccgag
     4381 ataaaggaaa ttatgtacgt tttggtattc ttcagctatc tgtttgatat ggtcattcgc
     4441 ttcaagcact tgttttacct tttcaggtat tttactcaat tcaacagtaa gcgattgata
     4501 tttactttcg gtaatagtgc ctctttttct ggcaatggca agtgctatta gtgtaagtac
     4561 agtaacctgc gcggtgaatg ctttggtact ggcaacacca atttccggac cagcatgcgt
     4621 atatgatcct gcatgtgttg cgcgcggaat gctggaaccc actacattac agatgccgaa
     4681 tattgttgca ccttttgatt ttgccaattc aattgcagca agtgtgtcgg cagtttcacc
     4741 actttgggaa atagccacaa caatatcgtc ttcggtgata acaggattgc gatatcggaa
     4801 ttcagaagca tattccactt ctgttggtat acgcgcaaga tcttcaaata aatattcgcc
     4861 aacaagtcct gcatgccagc ttgttccaca accaatgatg atgatgcgtt tggcattaac
     4921 gagtttcttt tcatattctt taatacctcc catcacaata gcattgcgtg aacctatcaa
     4981 acgaccacgc atggaatctt gaatagaatt tggttgctcg taaatttctt tcaacatata
     5041 atgatcatat ccaccctttt caatcatatc cagttgcatt tgcaattcct gcatataagg
     5101 ggtgatattt tcattatgta tggtaataat ttctaattct ttatttctgc gaacgatggc
     5161 aatttcttca tcattcaaat acaccacgtt gcgtgtgtat tcaattaagg gactggcatc
     5221 agaggcaacg aatgtttcat tatcgcccac gcccaccacc ataggagatg attttcgtgc
     5281 tgcgataagc atatcaggtt catctttcga aacgatgaca atggcatatg caccaacaac
     5341 ctgatttagt gctacacgta cagcttcaac aagatctaat ttctcgttga tttgaatatc
     5401 ttctatcagg tggatgagta cttctgtatc ggtatcggaa agaaatttgt gaccccgctt
     5461 acttaattct gttttcagcg gtgcgtaatt ctctataatg ccattgtgga tgatggccag
     5521 gtttccgctt tcgctgatat gcgggtgggc gttgcgcgta ttgggttcgc cgtgagttgc
     5581 ccaacgtgtg tgaccgatgc caagagtgcc ttgcgtattt tgatcttcgc aaactttctc
     5641 aagctccgat actttccctg caatcttata tactttcaga tcgccattga tcaatgctac
     5701 tcctgcactg tcatagccgc gatattcgag gcgatgcagg cctttgatca gaaccggata
     5761 tgcttcacgg tgtccgatat agccaacgat accacacatg ttttatttgt ttatggttgt
     5821 atatgtaacg gtaaaacgag gtttaaaatc ctcacgcgga cacattggcc ctcctaaagt
     5881 tacagcattc ggcacacgat tggtgggata acaaattaat gcaaacccat tgttatcgag
     5941 tgaaccttca ataatctcct gcacgtgctt ggtgatattg tattgatatt cataaactgt
     6001 tcgcccggta ttgtcgcgcg tagtttttat tgcaacgccg ccaatattcg ttgaaccaaa
     6061 attatcatca acgatagagc tatataattc gtaggtatag agagcgaggt aataataatt
     6121 acgaccggta gaatccagtt gcaccaattg cacttttggc ggtgcaggat aataacgcga
     6181 tccatcgttg gtcatggtga gtaccaattc cgctttatta atagacacat cctgcagatt
     6241 ggtgagagtc ggtaatttca caattgttct ggctcctgca aaacctttta cataggtaaa
     6301 tgcatcgccg tcagtggtat cggcattcat gatagcatcg ccgatatctg tgccgggatg
     6361 tagctgattc gtgaaactat tgcattttgt tccggtaagt ggcaacatca cacttaatga
     6421 atctgcttca gaattatgat aataaatgcg caatcctgaa atatcggaag ccatatcaaa
     6481 atacatgata gcatttgaat aaccggcaga agtatcaggt gtgatataaa ttccctgaag
     6541 gtattcgagg aatgaagtat ctgatgcaag aattgtggaa tccagattaa tgatattcgt
     6601 tccgaaggtg ttatacatag gaatgcgcaa atgcggttcc cataatgagc tgtcggccag
     6661 cattacactg tcgagcatat tcaccttgaa attagattta cgcccaatcg gcacagggat
     6721 atggcggaat ttatcgtcgc tgtgataatt tcttcccgac tgcatgggct ccgacatacg
     6781 ataaacattc agactatggt agagtgtagt atctccataa tatccggaat aatcgagtgt
     6841 caatactacc gaatccactt caggtgcatc gccgaagaag aggttattgg caggaagaag
     6901 gacttgtgaa tagatgcttg ccgtagcttt tccataacgc tgattgtcga gcgagcctaa
     6961 agcgtagaat acattctggc tggtcaccac cgaatcatcg aaatcggtat aggtgataat
     7021 tttgaaggta tctatctgtt cggaattgag gaaatcctca tccggcaaca ggtcctttcc
     7081 aataaaattg tctttgtcgc aagatgtcag gatgacaata catccacaca tcaacagagt
     7141 cagcgcacgg ttcatcctgc ctataacgga gtaaatcaca aattattgat agagttcatt
     7201 ataaaatgcc gcatatttct cgagatatga ttctttagca tattcgagta ccggaatgcc
     7261 caatgatttc gcgtatttgc cgagttcttt ttccaggtct tcgctaccta tgatcacgcc
     7321 atcggcatat ttggcaccac ctttactgag tccgatatgg ttgtattctt tgtactcatt
     7381 gaggtgatct gcttcgatat gggtgctgat aggcgctttt tcaaggaatt tatcctttgc
     7441 cagtttttca tcaaaagcac ctttatacat cgagtaaacc acttttgcgt tctggaatac
     7501 aggttcgttt ttgtaggtag ttttgatata cagcggcacg aggctggtca tccaaccgtg
     7561 gcaatggatg atatccggag cccattggaa ttttttcaca gtttcgagtg ctgccttaca
     7621 aaagaacacc atgcgttcgg cgttgtcttc gaaaaacttg ttgttatcgt cgtggaacac
     7681 atgtttgcgt ttaaacaggt cgtcgttatc gagaaaatac acctgcatac gcgctccggg
     7741 aacagatgct accttaatga tcagcgggta atcgtcgtcg ttgatgatga tattcattcc
     7801 gcttaatcgc accacttcat gtaggcgatg acgacgttca ttaatagttc cgtagcgggg
     7861 cattaatact ctgatttcat ttccctggtc ctgcacgtat tgtgggagtt cgcgggcaat
     7921 gtcgccaata tcggtaccat tatgataagg ctgcatttcc tgggtaatga tcagcacacg
     7981 cttcttttcc ataggcaaag atcttgataa gtcctgcggg gatcgtaact ttccccgttc
     8041 tcctcagaag gttatttttg aggtgcaaag ttacaaaata gtattcagta ttatattatg
     8101 caacctaaag tgagggtatt ttaacaagtc ttcactttcg gctgaaaccc ttatcattgc
     8161 aagcatgcag gtcattacca cacgttcagc ccttacccct ctcctattgc gtgaaaaggc
     8221 actggggcgc aaaataggct tcgttcctac catgggagcc ttgcacgacg ggcatttgag
     8281 cctgatagcc gaaagtcgca aacgagccga tattaccgtt tccagcgtgt ttgtcaaccc
     8341 cacacagttc aacgacccta aggacctggc caagtaccct cgacaacccg agaaagacgc
     8401 tgcaatgctc gaaaaagcgg gttgcgacta tctatacctt cccgccgtag aggatgttta
     8461 ccccgaagga cccggaaaag ggcctttttt cgaccttggt ggcattgata cccgcttaga
     8521 gggtgcctca cgaccgggac atttcgccgg ggtggccgaa gtagtgaagg tgctggtgga
     8581 aaccctggtc ccggatttcc tgtttctggg tcagaaagat tttcaacaat gcatggtagt
     8641 gaagaggatg ctcgatcaat gggataaaca ccattgggac agcgcgctac aagaggctag
     8701 tctcaactgg cgacccggag atgttgaatt ggtgatatgt ccgattataa gagaagcaca
     8761 tggactggca atgtcgtcgc gtaatgaacg actatccggt gaaatgcgca ccagggcttc
     8821 agccatatat gcgagtattc aatacgcgga ggaattattc cctacatttt ctgctgatga
     8881 aatcagcact atttgtgcta acgccttaaa ttccatacct gatttccaag tcgattattt
     8941 tcaaattctg gacggtgaaa cattatcacc ggcaaatgca gacacacaaa aatttgtaat
     9001 tattacagca gtaattgttg caggagtcag gttgttggat aataaaatta ttataaattg
     9061 attttaatag cagtgagcta ttttcaacct aaaataaaga cacccgccaa cagaaaaccc
     9121 agtgccgaaa aagtgattat aatcttattt ttcaaggaag tagaaacatt gaatatctcg
     9181 cgaacttcca ttcgtattaa tccataatta agtgcaagaa ccaatatagt tccaagcagc
     9241 aatgtggcat ttgattgccc tgaatcagta tattgaataa atggttccgg tgcaaggggg
     9301 gataaaatca attgtccgat cataaggcct gctgttgcag caataataat gcctatcatt
     9361 acccaacctg ccttttttcg catgaaaaag aatatgatag caataatcca aatcaccaaa
     9421 ggaagcaaag aggcaaacgc atctaccaac atcagatcat cccggcttac catttgaatc
     9481 aagtatcgat tttgatagaa agccgcaagc accattattg aacaaatgat tccgattatt
     9541 ttcaccagac gatcacctcg ctgtggctct gtttgacccg gaataaattc gcgcgctata
     9601 ctatcaagtg catcggaaac aacttccatt tggcgttgtt tcaattcttg ttttttccgg
     9661 atttgacttt ttctttcatc caatacagat tgcgcctcag ctaactgttc atcggttaat
     9721 tgccttaatg acaattctgc ttttgcagcc ataaccgctt ctggcacata gtcatcagga
     9781 aattgaatta cttccagtaa ctcgtcgata gaaaaattct tgtagcgttg ctcgaaaggg
     9841 tattccataa ttgcttgttg ctaaattaag aaaattagcg actagcagca attaccatcc
     9901 tggaatggaa atttacttcg ttcgatataa caaagccgtc aataaattga cgtgtaaaaa
     9961 aagccttgga aattatttca ctgtaaggat ataattgacc attttctcgg catcagacct
    10021 actcagattt ggatgtgctg ccattgccac atcaccccaa acgcctgaac ctccgttgat
    10081 taccttgtcg accaacatat tcgccgcacc ttcctgacct gcatattttg ctgccacatc
    10141 tttgtatgca ggaccaacca atttttcctg ttcaatatga caggtgaggc aatcattttt
    10201 tgtcaggagt gcctttcctt ccaatcgttt agcggtttcg agatttttta cggcatcggc
    10261 acttggttta gaaccactgc caccgcatga agcgaggata aggcatactg agagggctgc
    10321 aagaagttta cgcatatcgg taatttttac aaacacaaat gaccgacata ctgtggtttt
    10381 cgcaaatgac ctatactact tccgacagcc tgacatatgt caattgaaaa atgacataca
    10441 cactatttca aacaattttt gttataaagc ttttccattt catcaccata catagtagta
    10501 aaacctttat tgagtgcttc agtaatgtcg tcacatgctt tgtcttgttt atccatgcgc
    10561 agataaatta atgctctatt tctgtaggca taagaatttg cgggatataa tttgatagct
    10621 ttattgacat cctccatagc accttttaaa tcgccggtca tcattttgct gtatccgcga
    10681 ttattatatg caacaccatc gtttggatcg gcttcaagca ctttattaaa acatttgatt
    10741 gctttatcgt gctgtccatc gagctgatat ttgaatccga tatttacata taccatcatt
    10801 tgggtgctat cgagcgagag tgctttttca taatatttca aagtaagttc acccttacct
    10861 atttcatctg caatcacgcc aaggttgatg tatgccgaca catcgcttga atctaattta
    10921 acggcagcca gcaaatcgtc gtatgccatt tcaaactggc gtatcgacat atatgcagca
    10981 gctctattca ccagcgcgaa acgtttaagt gtgtcgtcag gagcatattc atacgcaata
    11041 gacaaatctt taattgccgc atcgaattcc tgaagagaca aaaggaatac acccctgtga
    11101 ttaataagtt cgtttttaac acctgttcgc tgtatgccaa gtgaataggc gtcatatgct
    11161 tcctgatatt tttttaattc aatcaacgcc atgccttttg cgatataggc ttcaaccaac
    11221 attgagtcgt tcttgatttt accgtctgca cattttaatg ctgcctgata ttgtttggca
    11281 tcatagaggt cgaagcattc ggaggcttta tttaacggag tttgcgcgct gagagaaatg
    11341 agcattccca ttgcaaccaa gaaacatagt gttttcatgt ttgagatttt atacgaaaat
    11401 atgagattcc ggttagaatc gcaagttcat caaattcagc caaattaata agtgaaggaa
    11461 agtccggcac cggcacccat catattgcca taattcacag atagtcctaa atatggtttt
    11521 attacatatt ttaagcctac ctcatactcc atatcggtat ttcccatcac aaacagtttt
    11581 aagcgaggtg taataggaat atcatctcgc atcagttgaa cgcgcaaaga accgtctaac
    11641 atgatatctg actgaagagt gaaattaaat ggaataagat atgtaaatcc aattgcaggc
    11701 aacaaagggt cccgatcaat tatattctgg ccgaaaatat tctccattgt ttcgtcatgt
    11761 ccgaatctgc cagatttgta cattaaccct atgtaaggca taaaccattg attactccca
    11821 ataaatctac ccagatgtat ttctgattca tatgtcggat gagggttgaa aggcaccatc
    11881 caatcagcac taaactgcca ccgagtgttt tgaatcattg ccataccgtg actgccgtta
    11941 tttgccaaaa atccatcagc catgaaatgc agtgctttat cctcattata aaattttcgc
    12001 ctgctatagg cattctgaat aaatcttaat gtatcggtat cgtcttcgat agtaaacacc
    12061 cttcccatac cactcatcat atgatacagc aaatggcagt gaaaatacca atcaccatca
    12121 gcatttgcca aaaattcaat ggtatcggtt tccataggca taatatccag cacattctta
    12181 agcggtgcat agtttccttg gccattcaat attcgaaaat cgtgaccatg caaatgcatt
    12241 ggatgtcgca tcatagaatt atttgtaagc actattcgga ccaattcatt tttgtgtata
    12301 agaattttat ctgcttctga aacaactttg ccattaatag accatacata tcgttgcata
    12361 ttaccggtga gtgtaaaatg caatgtttta attgaatcat atcctgtgat cgcagtattg
    12421 gttgtggcct taagcatact atagtttaag gtttggggaa aaccgattgt atgggtattt
    12481 tgcatttcat caacttcatg gatatgatca atagcaacat tttcttgttg gttcaattcc
    12541 gggtacataa cttcattcat atccataacc tgcataccca ttcgcatgcc catagcatcc
    12601 atggtgccat tcatgcgcat cataccattc atcattttca tgccttcgaa atagctgagc
    12661 ttaggccatt tataggcaaa tattgtatcg cctgaaccca gccatatact cgctgattgg
    12721 gatcgatctt ccgacatcac aagaaattcg tatgtatgcc cggtcggaat tttaacaatg
    12781 atgtcatacg tttcagaaac tgcaatcagc atccgatcta cttccaccgg ttcaacatca
    12841 tttccgtcat tagcaatcac cataaccttt ccacccgcgt acgataacca ataatatgtc
    12901 gaagcagagc catttgcaat cctcaatcga agtaaagagt ctgcaggtaa taactgaaag
    12961 gatgtttgtc cattcacgag tatgtcatcg taaaaaacat cgctcacatc cattgcgttc
    13021 atgcgcttcc attcattaga caactttact ccgagtgcat gattacctat tgcctccgca
    13081 tagctttgcg cagcatgttt tttaattgta aaccagtcag aaccacctct caataagtgg
    13141 tgaatgtagt ttggatgcat agttgtccat tcacttaaaa ttatttcctg attttgaatg
    13201 tcatcaatac ctgttcttat ttgcgcatcg tctgaccgtt tgttgaaaat gagcatgcca
    13261 tacattccta tttgttcctg gagacctgag tgactgtgat accaatgtgt tccattttga
    13321 atgaccggaa aacgataagt ataggttgct cctggttcaa taggcaattg cgtcaggaaa
    13381 ggcacgccat cttcagcatt tggcaaaaat accccatgcc aatgcaagga agtactttca
    13441 gttaaatgat tgaaaactga aatttctgcc gtatcacctt cagtaaaaac gagagtaggc
    13501 attggaacct gaccattaac cgcaatagcg cgaacagact ttccattaaa gtaaagcgtg
    13561 gtatcggtaa tatgcagatc ataatgaacc actcctgtaa aggccttgga gcaatttgaa
    13621 ataatcaaaa ggaagaaaac aaaagctctc attgtaaaac gtttaaattt cttattacac
    13681 cacattcttg catactttta tctacatagg ggtttatgac tgaactatca taatctatcc
    13741 aatatgcctt ttgcatggta caataaaggt aattgtaatt taagttgaat gcaggagatg
    13801 aagaaacaaa attccaaaca gcaggagtta atttagcgaa agcatttctt atctcattta
    13861 aatcggaaga tgatgaaatt ttacttgcat acttatacat ttcagacaca ttttcaagaa
    13921 aagccgtttt ttcagtctcg cttgaatcat cataaacaaa taaggctgct ttcaacaagg
    13981 ctcctgcttg attagcctgc acgtaattcc cttcagacaa tgcttcttta acaaccatat
    14041 atctattgag tatttgtccg gcactgacgc cttgagcaat tgagatgcta tgtattatcg
    14101 caatcagaga aaacaggata aagcttttca tacgacaatt atttaatttt ttcaatattg
    14161 aatcctgctc ttttaaccgc agaaatcacc tcttccattc gtgcttcggg agcagtaaca
    14221 gatagaaatt tatcggggac catcgtatta acactccagt gcgacaactc ggtgagcttt
    14281 tctaattcaa tcttaacacg agccacgcaa cccgaacagt taatattagt tttaaatgtg
    14341 tttgtgataa ccattttatc aatttgattt tccataatat ttgtttttgc aaagttgatt
    14401 cagcaattgc tgacacctat tatacaattt attattttaa ttacatgatt tacatgccga
    14461 tgtgcgacca cttaagtctg aggctattgc cgacaacact aatgctactc aaagccatag
    14521 cagcagctgc taacatggga tttagctgaa atcccgtgat tggtattaaa atccctgcag
    14581 ctattggtat tccaataata ttatatataa atgcccaaaa aagattttgc cttatggctc
    14641 tgactgtttt gaccgacaat ttaattgcgg cgggaacctt caataaatca gaagacatca
    14701 atgtaatttt agcgacatca agtgcgatat cacttccggc tcccatggca atgctaacat
    14761 ctgactgagc aagtgctgcg gtatcattta ttccatcgcc aaccatagca acttttttac
    14821 cggcctcctg caattttttg atataggttg ctttttcagc aggaagcact cctgccttta
    14881 cataggttat acctacttca tgtgcaattg attccgcaga tatttgattg tctcctgaaa
    14941 gcatatgcac ttcaatgccc atttccttca actgacttat agccattttg gaactgcttt
    15001 tgatttcatc tgatatagca attaatgcat acacttgatt ttcatcagta aaccaaacta
    15061 ttgaactacc ttgactaact aattcattcg cctttaccat cagcgtgtca gatatactga
    15121 ccttattctc ttgaattaat ttactattcc caacaaacca atttttctct tgatactttg
    15181 ctatcattcc cttcccgccg actgcagtgc ttgtttccaa tgcaagaata gaactttcgg
    15241 gcattgattg aacaattgca ttggcaagcg gatgtgaaga atatttttca attgtaaata
    15301 gtaattgttt gtaaatagga ttgtcatcaa accaaatgat ttcagagacc tttggttcac
    15361 cccgagtaat tgttccggtt ttatcaagaa ctacagctgt tatgtctcta gcttgttcga
    15421 ggctcgcggc atctttaatt aaaataccat ttgcggcacc tcttccaact ccaaccatta
    15481 ttgctgtagg tgttgcaagg ccaagtgcac atggacaagc tattactaaa actgttgtca
    15541 ttgcaagcaa gccctgagta agcccgtttt cgcctccaag aacccaccat agcaagcctg
    15601 aaagcagggc taagagcata acaattggaa caaaaatggc tgcaatttta tctacaagct
    15661 tttgcacggg ggctttgctt ccctgagcat tacgcaccat tgaaattatt tgtgccagca
    15721 tggtttcaga acccttatgc gtaactttca ccttcaatac cgcggaccca ttcaatgatc
    15781 cggcaaacac cttatcatct ttagatttaa ataccggtat tggctcaccg gttaatgcgc
    15841 gctcatcaac aaatgattct cctgcagtaa taatgccatc caagggtatt tttgctcctg
    15901 gctttattac gataatttca tcatgctgta cattgttaat ttgttcatta cggacagatc
    15961 catctttctg taaaacctga acagatgatt cctgcaaacc aataagtttt tttatagcag
    16021 atgctgtctt tcctttggct cgttcttcaa gcaatttgcc taataaaata aaagcaataa
    16081 tcactgaagc tgtttcaaaa taaacatggg aatcgagacc gcgggaggtc caaaaagcgg
    16141 gattaactgt attgaataca ctgaaaatat acgatatacc tgtactgagc gccaccaacg
    16201 tatccatatt tgcctttcgg tggatagcct gcttccatgc attcacaaaa aaactacgac
    16261 caaaccataa tactacaggc gtgcttagca accacatcaa atagttagcc cgaggcatat
    16321 ccataaacac catgctgatt agtatcagag gaatggatag cactactgcg ccggcggtgc
    16381 ggttaaaaag acggaggtag ttcttttgct gtatttgttc cagagtatct tcaacattag
    16441 catcaacaat caacaaatca tatcctatag attgaaccgc cgcccggatt tgctgtggat
    16501 ttataatagt aggtatatac tcaaccaata gtgaagcatc agcaaaattt acaactgact
    16561 ttattacccc cggctgagct ttaacaatag attccacgct tattgcacaa gatgcgcatg
    16621 tcatttgtaa taccggaata ctttgcgtta tggttactac atcataacca attccgcgga
    16681 tgcgctctac gccggctttt atcacatccg gtgcatttgt tgttttgatc agcgcccttt
    16741 tattgttaag ttcaatctga acctgatcaa tgccgggtaa tttg
//
LOCUS       DAOQOU010000052        16738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1724_length_16738_cov_5.789127, whole genome shotgun sequence.
ACCESSION   DAOQOU010000052 DAOQOU010000000
VERSION     DAOQOU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16738
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1724_length_16738_cov_5.789127"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLM90_06380"
     CDS             <1..223
                     /locus_tag="PLM90_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ89032.1"
                     /translation="PKHYHAAHTEQVFVLEGHATMLLGDVWLDVAPGQQIFIPKGTPH
                     SVKVKEGEVLKVISIQSPMFDGTDRIKLE"
     gene            complement(230..691)
                     /locus_tag="PLM90_06385"
     CDS             complement(230..691)
                     /locus_tag="PLM90_06385"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HMZ89033.1"
                     /translation="MWRFLTFLAINFGALGIGSLLMGSIPNNTWYNDLNKAPWTPPGW
                     VFGAAWFTIMLCFSIFLYIVSGKYSAAVLRPLYLLFAIQFVLNVMWNPIFFRWHMVMP
                     GLVVILSLTIIVALIIRWGFTNHSQATWLVLPYLIWLCIATSLNAYVLLKN"
     gene            complement(738..1664)
                     /locus_tag="PLM90_06390"
     CDS             complement(738..1664)
                     /locus_tag="PLM90_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ89034.1"
                     /translation="MENRKQTTILLIEDNPADARLVEIYLNESAMIQPQISRVSELGK
                     GMLMLEERDFDIVLLDLTLPDSSGFETVKTILREFPGQTVIVMTGLEDDTVAINSVKA
                     GAQDFIVKGQFDSNLLGRTITYAIERHRLQIKLEDYAKAIKLNEQRLTEAQKLAQMGN
                     WELDVVNNQMYWSDEVYRILGYKQGQLEPTLADYLKPIADDDVEEVKAILNRTLEKGQ
                     PFHAEYKVKGPGNSTKKVSIQGLVQKSKINGGLCLAGTIQDITNLSLAKSEGIDKQAL
                     MSVLKNAIAEADNSGASASLKKLLESGLNMIK"
     gene            complement(1674..2126)
                     /locus_tag="PLM90_06395"
     CDS             complement(1674..2126)
                     /locus_tag="PLM90_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ89035.1"
                     /translation="MRSHNEDVMQILLVEDNPGDIRLTQEAMKEGPFKSELHVVKDGV
                     EAIEFLRHKGKYSLSPVPDIILLDLNLPRKDGREVLAEIKNDEVLKLIPVIILTTSDA
                     EQDIYKSYKLNANCFITKPVDLDQFIYIIRQIETFWFTVAKLPGRRSA"
     gene            complement(2218..2904)
                     /locus_tag="PLM90_06400"
     CDS             complement(2218..2904)
                     /locus_tag="PLM90_06400"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HMZ89036.1"
                     /translation="MPKRILFILLFTVTLGYAFSPAPATAPTYSIALLKYNGGGDWYA
                     NPTSLPNLAEFCNKNLNLNIATDIPTVEVGSPELFNYPFVHMTGHGNVVFSAAEAENL
                     RTYLIGGGFLHIDDNYGMDPYVRPAMQKVFPELSFVELPFSHAIFHQHYEFRTGLPKI
                     HEHDNKPPQAFALFWEGRMVCLYTYECDLGDGWEDRDVHKDPEETRQKALQMGANILQ
                     FVFMENTPNG"
     gene            complement(2897..3607)
                     /locus_tag="PLM90_06405"
     CDS             complement(2897..3607)
                     /locus_tag="PLM90_06405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HMZ89037.1"
                     /translation="MHCAYLPDYFTGDTAELPDVEIHHLLHVLRADIGTKIRFSNGKG
                     LTGNADITLLNKKQGHIHITDATHHQRLSPSLHIGVALLHNSDRMAFLMEKLTELGVD
                     RITPLLTSNTERRHFKRDKELSHMVAALKQSAQPFLPQLDDVVQFLQWLPQVSKTADR
                     RIAHCRKTGMIPLAKTCSENAICIAIGPEGDFSEAEVAAAIDHGFIEVSLGNEVLRAE
                     TAAMSAVMTIKTWRTLHA"
     gene            complement(3614..4477)
                     /locus_tag="PLM90_06410"
     CDS             complement(3614..4477)
                     /locus_tag="PLM90_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ89038.1"
                     /translation="MKRIITLSICTIISAFLHGQLPVFSEHAITPSSINPALTAYHSD
                     ALIHAHYYNTFSGAIDNATTANIIGEYSFDKNKAGIQLMHDNFGVDIQNIVSLNYARH
                     FKVANGTISGGVSFTGKNIARELSKTHPASSGDPTLQADISKTLFNIGAGVAYITNDA
                     FIAIGTPGFMQENIAEHDNNTDASSEIPLFINGAYRIPLNDTWNITPAVFTALENGQP
                     LILDLQAAITWHEHLKATIGYGNDHSWLAGIHARFNDRYTLGYIYDMHLPLGVASHEV
                     MLGFDLNSSIF"
     gene            complement(4474..4827)
                     /locus_tag="PLM90_06415"
     CDS             complement(4474..4827)
                     /locus_tag="PLM90_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ89039.1"
                     /translation="MKLNGLFLVSILCVVTNTVIAQTACADPVIPDVITPNGDGINDK
                     WEISCLAYFPDNHLTVYNRYGKIVYEADGYDNNWDASWNIDKGPLPDGTYIYLFVVNQ
                     TDNKKTYTGSLTVAR"
     gene            complement(4824..5627)
                     /locus_tag="PLM90_06420"
     CDS             complement(4824..5627)
                     /locus_tag="PLM90_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ89040.1"
                     /translation="MPKIAWISALCVVAVLATGFTPPHHGKTGRFLKRHGFMYIPGQA
                     DGMNHDFYIQAYEISNENYRYFLNDLQIKGRYDELRIAGVDTAQWDAIFGDSSAYSKF
                     YFQHEAYDHYPVVNISYEGAVMYCKWLTEAYSLRGFNIEVRLPDSTEWKIAARGGEAD
                     NTYGWGKDSYISKQGYYRCNAKKGDTCSLTLTPVMAYTTNRYGLYNMSGNTTEMLLEK
                     GKHKGGGWNSPEPNIRIDGPDDYAGISGPSPFIGFRPIAIIHDEKTTAK"
     gene            complement(5712..6524)
                     /locus_tag="PLM90_06425"
     CDS             complement(5712..6524)
                     /locus_tag="PLM90_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ89041.1"
                     /translation="MKYLTSTLLILCLITLTTSFSPQKQAKVMKKQGFVFVPSGATTV
                     DGINLTINGFYMQAGEVTNKEYRTFLEDLKAKGKTAEYEVANVQSEKWILPYSSLEEM
                     KEHYFTHPAFDNYPVVNISQQAARLYCTWLEEQLKANGVGAKVRLPEKAEWVYAAHGG
                     IQGSIYPWEGNELRNKKGIYLANYKTSSLSEDGAYMTAISKSYFPNGFDLYNMSGNVS
                     EWISTDGETKGGNWWSEPEYLRIDAAQEFPYSTEASPFIGFRTVVTVTSLVK"
     gene            complement(6623..8233)
                     /gene="pckA"
                     /locus_tag="PLM90_06430"
     CDS             complement(6623..8233)
                     /gene="pckA"
                     /locus_tag="PLM90_06430"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HMZ89042.1"
                     /translation="MESGIKNPAASLAAIGLNHTGHAFWNLSPAELVEHTLQRGMGVM
                     ADSGALVVDTGKFRGRAPKDKFTVKDEKTTNTVDWNNFNIPTDNATFDKLYAKVLAYL
                     SDKDIWVRDNYACADPKYRINIRVINEYPWGNMFCDNMFMRPTAEEIKTMNPDWLIIN
                     APGFQADPAVDNTRDKNFAMIDFTKRIILIGGTGYTGEMKKGIFTVLNYWLPQEKGVL
                     SMHCSANVGKEGDVAVFFGLSGTGKTTLSADPDRRLIGDDEHGWTEEGVFNFEGGCYA
                     KCVNLTRENEPQIFNAIKFGAILENVEFIPGTRTVDFTNITKTENTRVSYPIHFIDNI
                     MVPSKGGLPKNIFFLTCDAFGVLPPISKLTPGQAMYQFLSGYTAKVAGTEAGVTEPQL
                     TFSTCFGAPFLPLHPTKYAELLGEKMRKHNVNIWLINTGWTGGAYGTGKRMKLSYTRA
                     MITAALNGELDSVSYATHDVFGLHFPTTCPNVPTEVLDPRNTWSDKQAYDAKANNLAA
                     QFVKNFEKYADKANAEIMAAAPKAGVSA"
     gene            8472..9116
                     /locus_tag="PLM90_06435"
     CDS             8472..9116
                     /locus_tag="PLM90_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89043.1"
                     /translation="MHKSPRILLSFAFFLMVSGVYAQAFSTLKLTSLHNYEIVDDAFN
                     RSLVGLSPEIGYEAYNYSYQLEIAAYIPRQIDSMVYVYDSLFGYGFDAPVSETMTIFT
                     IGLMSRYYLFSDWMPVNRLYTVSGIMGVFEHVNQDFIAGGLSEGKKFTNKSLDLTFGL
                     GFETLIDDYLMPFIETSVYFPVAQGENPYNTVYDITEGYGVRIAGGVKLYLSDM"
     gene            complement(9186..10292)
                     /gene="dprA"
                     /locus_tag="PLM90_06440"
     CDS             complement(9186..10292)
                     /gene="dprA"
                     /locus_tag="PLM90_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HMZ89044.1"
                     /translation="MYHLIAISLIPGIGPVLARNLVSYCGSAEAVFKVKRSHLLKVPG
                     IGEAVADNIRRFSDFDRVEQELRFIEKNEVQCLTYLDELYPRRLKQCPDAPLLLFSRG
                     NADLNAPRILGVVGTRNATSYGKGICEDLAASLKDENILIVSGLAYGIDIAMHKACVK
                     NNVPTIGVMATGLDRIYPPAHRETANKMMTNGGLLTEFMSDTNPDRQNFPRRNRIVAG
                     MIDGLIVVETGINGGAVITALLADDYHRDVMAFPGQIHHPYSTGCNKLIKSNKAAMVE
                     STEDVLDIMGWSSNTTKTQHVQQQLFHDLNDDEQQIHTILQEYGELYIDDIQSKSQLT
                     PGNLAGVLLSMEFKGLISALPGKRYRLERVAIQM"
     gene            10350..13679
                     /locus_tag="PLM90_06445"
     CDS             10350..13679
                     /locus_tag="PLM90_06445"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ89045.1"
                     /translation="MRHYQTIFVTFFCITFSLTAYGQQILGDNFNRPAYDYRSEKNPY
                     YWKNKKPFDGYWQQDVHYKIDAKVDEKTDIISGKVELTYYNNSPDALPFVYFHLYQEA
                     FQPDSYVETLNEVNNVKPTYGKYEAEKKGCDVTEITVTARNGKKVSESTKLTQDITVL
                     KVELNEPVLSGQSITFSIPFNTYFDSGSLRRRMKKFDSGGFEHYDGVHWYPRICVYDR
                     KKGWDTDQHLGKEFYGDYGSYEVTLDFASNYVVEGTGWLENEAEVMPKSLREKLDIRN
                     FAGKPWESPASVVIPYIDGDRKVWKYYALNVHDFAFTADPNYRIGETMAVINGQTVRC
                     IALAQEGHAGFWQNAADYTKQIIETFSADFGPYIWPKIIVADARDGMEYPMLTLDGGY
                     NPAYYDLLIHEVGHMWYFGMVGNNETYRASMDEGFTQFLTGWGYRKISGDYRIDFPEQ
                     NKYVAKYRNPDKIMMSEVYAAYLSDAAREADATLNTHSDMFNSALAHGGGYRHVYFKT
                     AVMLYNLQYVLGDELFLAAMRNYTDDWKLCHPYPEDFRNSISQYTHTDLDWFFDQWLE
                     TTKNIDYGIKKVDKVSSTNVDGQTLLNYEITLDRKGSMQMPIDFSVVLDNGDTLRYYI
                     PNTWFNKYEYQKPSQGRLDRTYFQNVISLPKWYGWDKLNDTYVASITTPHKIKDVIID
                     PSDRLADINKLDNSWKCPTEWVFDSQIRNAPDWDHYTMRWRPEIWGNAYDGLKVGLHL
                     NGDYFAYKHKLSLTAWYNTGLAQGALYDNEFLGNTLDNANEDISDKFDKFNFDFNYRT
                     STDRFLPLSDFILNTKYLDGVFVFETGFEKKLGENNRDLLKVYVKDMQFNNIEYLYGP
                     YVIEGNKNNTVNIDYTHRYNYFTGNGTINVRFRSNDLLSDYDFSRLQFEAINITKLGK
                     LDLHTRYFAQWGYGSNVPFESKLMLGGANIEDMLDSKFTRSRGFFPDEWGEFGTTTNH
                     LQMGGGLNLRGYSGYFAADDIDGETQLAYAGLSGTSLSAELQFDRIVKKQLIRFIKME
                     PYLFADAGIMVGDNAFEEISKPRIDAGIGSAFTWSWWGPLETVKPLTVRIDLPLFLNR
                     PPAEDPEYVKFRYVVGINKTF"
     gene            complement(13747..14391)
                     /gene="rsmG"
                     /locus_tag="PLM90_06450"
     CDS             complement(13747..14391)
                     /gene="rsmG"
                     /locus_tag="PLM90_06450"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HMZ89046.1"
                     /translation="MAILVSVEHILRYFPELSPLQISQFKQIGAQYKFWNEKVNVISR
                     QDIDNIYQHHVLHSLAIAKFISFEPGTKVLDLGTGGGFPGIPLAIMFPEVEFHLIDSI
                     DKKVKVVESIYKALGLRNVTVDQVRAEDIREKYDFVVTRAVADMMILYDWTINLIRRG
                     HSVNDVPNGLIMLKGGFLDLEIAPFKDILYKFPITEWIPEAYFIEKWLLYLPMD"
     gene            14478..16064
                     /locus_tag="PLM90_06455"
     CDS             14478..16064
                     /locus_tag="PLM90_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMZ89047.1"
                     /translation="MAIRMVPDDNQPQQQRKLPGTGGRGGSGGGGGNLITMLLPLLLG
                     IFRKNPKTGCVTLIVIAAVIYFMGGNLGGLLNPGGGGTNITDLFNTGASFDPVKYDAT
                     EIFEPPITDNAKNPLPDAVSLLRYAPTRRNQGSQGSCVGWGSAYAARTILESERTGRN
                     PDDIAFSPAYLYNQIGLEGCQGAYINNAMEVMKDQGLVTLREFPYDDNNCTKQPTSSQ
                     KQQASQYRMNGFNRLTVGDASGTGNEKIDLVAIKQNLAQGAPVVIGMMVGGSFMQSMM
                     GQEVWHPTEQDLSMYGFGGHCMCVIGYDDYKEGGAFQIMNSWGKEWGDNGVAWVPYNV
                     FEHFTVEAYGVYPMGSADKPLGNDLNASFGLVMNDNGRHIPLVKDGENTFSTQSPVKK
                     GTKFKIEITNSKECYTYVFALDENKKSTVLFPYTPKHSPYCGITGTRLFPKDYSMELD
                     AVGSRDEMAIVLSKTPLDYQALNNAANAAAGNYSAKMTQALKGKLQSEVSFSAGDVIG
                     LKTVLAENQAVVMVIGIDKR"
     gene            16112..>16738
                     /locus_tag="PLM90_06460"
     CDS             16112..>16738
                     /locus_tag="PLM90_06460"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HMZ89048.1"
                     /translation="MTTQWRLTAMCLLCTVVMQINAQDTYIPFRTSLPFSLDGALDEA
                     DWQRTPVETAFMQQGPVAGATPTENTEVRIMYDNDYLYVGITCFDSEPDKLIRTELER
                     DFPIGNEDGTGVTIDTYHDKLTGMDFLSNTLDARWDAQVTQDGGGLSESFNTFWDAKT
                     KLYDFGYITEYRIPFSSLRFEETEQVTMGIRVARLIKRKNELITWPPCD"
BASE COUNT         4722 a         3393 c         3656 g         4967 t
ORIGIN      
        1 gccgaaacat tatcatgcag cacataccga gcaggtgttt gtgttggaag gccatgcaac
       61 catgttattg ggtgatgtgt ggttagatgt tgctcccggc cagcagatat ttattcccaa
      121 aggaacacca cattccgtga aagtgaagga aggtgaagtg ttgaaggtga tcagtattca
      181 atcgccgatg tttgatggca ccgatagaat taaattggaa taatacacat caatttttca
      241 gtaatacata agcattcaac gatgtggcaa tacaaagcca gataagatat ggcaatacca
      301 accaggttgc ctgcgaatga ttagtgaatc cccaacgaat aatgagcgca acaattatgg
      361 tgagcgataa aatcaccacc aatcccggca tcaccatatg ccagcggaag aatatcgggt
      421 tccacatcac attcaataca aattgaattg caaacaacag atatagtggt cgcaataccg
      481 cagcgctata ttttccggat acgatataaa ggaaaatact gaagcaaagc ataatggtaa
      541 accatgcagc gccaaacacc catcccggtg gcgtccaagg ggctttgttg aggtcattat
      601 accatgtatt gttggggata cttcccatca acaaacttcc gatacccaga gcaccgaaat
      661 taatagcaag aaatgtgaga aatcgccaca ttgggtttgt gcgaattgtt ttatatccgt
      721 cacctacaaa acaacaatta cttgatcatg ttcaaacctg attcaagtaa ttttttcaat
      781 gaagcagatg cgcctgaatt atcagcttcg gcaatagcat tttttaatac agacatcagt
      841 gcctgcttat caataccctc tgatttagca agcgataggt tcgtgatatc ctggattgtt
      901 cctgccaggc aaagtccgcc attgatttta cttttttgta ccaaaccttg aatggatact
      961 ttttttgtac tgttaccggg tcctttcact ttatattctg catgaaaggg ttgacccttt
     1021 tcaagtgtgc gattgagtat cgcctttact tcttcaacat catcatctgc aataggtttc
     1081 agataatcgg caagggtagg ttctagctga ccttgtttat atccaagtat gcgatacact
     1141 tcgtcgctcc aatacatttg attattcacc acgtcaagtt cccagttacc catttgcgcg
     1201 agtttctgtg cttctgtcag gcgttgttcg tttaatttaa tcgccttggc ataatcttcc
     1261 agttttattt gcaggcgatg gcgttcaatg gcataagtga tggttcttcc gagcaggttg
     1321 ctatcaaatt gtcccttcac aataaaatct tgtgcaccgg ccttcactga attaatggca
     1381 actgtgtcat cttccaaacc ggtcataacg ataacagttt gtccggggaa ttctctcaaa
     1441 atagttttta ctgtttcaaa gccgctgctg tcgggtaatg taaggtcgag cagaacgata
     1501 tcaaaatcgc gttcttcgag catgagcatg ccttttccca attcggaaac gcgcgaaatt
     1561 tgtggttgaa tcatggctga ttcgttcaga tagatctcta ccaaacgggc atcagccgga
     1621 ttgtcttcga tgaggaggat agtagtttgc tttcggtttt ccatgtgttt gaactatgca
     1681 gatcgacgtc cgggtaattt tgcaactgta aaccagaagg tttcaatttg tctgatgata
     1741 tatatgaact gatccagatc tacgggtttt gtgataaaac agttggcatt cagtttataa
     1801 ctcttataga tgtcttgttc agcatctgag gttgtcagaa tgattacagg gatgagtttt
     1861 aatacttcat catttttaat ttctgccagt acttcgcgtc cgtcttttcg tgggagattg
     1921 aggtcgagga gaataatatc gggaacaggg cttaggctgt atttcccttt atgtcttaaa
     1981 aattcaattg cttcaacgcc atcctttacc acatggagtt cgctcttgaa aggtccttct
     2041 ttcatagcct cctgtgtcag gcggatatca ccggggttat cctccaccag gaggatctgc
     2101 atcacgtctt cattgtggga cctcattgtc acggaattac ctctgaatta taaaattccc
     2161 ccacccccga ttacgaaagt aggaaataaa atttgtttag acaccctaaa aatcaaacta
     2221 tccattcggc gtattttcca taaaaacgaa ctgcaggatg ttggcaccca tttgcagtgc
     2281 tttctggcgt gtttcttcgg ggtctttgtg cacatcgcga tcttcccagc cgtcgccaag
     2341 atcgcattcg taggtgtaga ggcataccat ccgtccttcc cagaataaag caaaggcctg
     2401 aggtggtttg ttgtcgtgtt cgtgtatttt tggcagtccc gtacggaact cataatgttg
     2461 atggaagatg gcgtggctga aaggtaattc cacaaaggaa agctccggaa ataccttctg
     2521 catggcaggt cgcacataag gatccatacc ataattgtcg tcaatatgca gaaatccgcc
     2581 tccaatcagg taggtacgca aattttcagc ttccgcagca gaaaatacca cgttaccatg
     2641 gcctgtcata tgcacaaacg gataattgaa cagttcagga ctgccaactt ccacagtcgg
     2701 tatatcggtg gcgatattca gatttaaatt tttgttgcaa aattcagcaa ggttgggtag
     2761 cgaagtaggg tttgcatacc aatcgccccc tccgttgtac tttaataatg caatagaata
     2821 ggttggtgca gtcgccgggg caggtgaaaa tgcataacca agggttacgg tgaatagcag
     2881 tataaataat atgcgtttag gcatgcaggg ttctccaggt ttttatggtc atcacagcac
     2941 tcatagcagc agtttctgcg cgaagcacct cgtttccgag acttacttcg ataaagccat
     3001 ggtcaatggc tgcagctact tctgcctccg aaaagtcgcc ttccgggccg atggcaatgc
     3061 atatcgcatt ttctgaacag gtttttgcca atggaatcat ccctgttttg cggcaatgcg
     3121 ctatccttcg atcggcggtt ttgctgacct gaggtaacca ctgcaaaaat tgaaccacat
     3181 cgtcaagttg agggagaaaa ggctgtgccg actgtttaag cgctgccacc atatgcgaaa
     3241 gctctttgtc gcgtttaaag tgcctgcgct ccgtgtttga ggtcagtaat ggtgttatcc
     3301 tgtcgacccc taactcggta agcttttcca tcaagaatgc catgcggtcg ctattatgca
     3361 ataatgctac cccaatatgc agggaaggcg ataaacgctg atgatgggtt gcatcagtga
     3421 tgtggatatg tccttgtttt ttattcaaca gggtgatatc agcatttccc gttaagccct
     3481 tgccattgct gaaacgaatt tttgttccga tatcggcacg caatacatgc aataaatgat
     3541 gtatttctac atcaggtagt tcggcagtat cgcctgtgaa ataatcaggt aaataagcac
     3601 aatgcataag caatcagaaa atagagctgt tcaaatcaaa accaagcatc acttcgtgcg
     3661 atgccacacc caatgggaga tgcatgtcgt aaatataacc aagggtatac cgatcattga
     3721 atcttgcatg tatacctgca agccaactgt gatcattgcc atatccgata gtggccttca
     3781 aatgctcatg ccaggtgatg gccgcttgca gatcaaggat tagcggttgg ccattttcca
     3841 atgcggtgaa tactgctggt gtgatattcc acgtatcgtt caggggaatg cgataagcgc
     3901 catttatgaa caatggaatt tccgaagaag catcggtatt gttgtcatgt tcggcaatat
     3961 tttcctgcat gaaaccggga gtgccgatgg caataaaagc atcattggtg atgtaggcaa
     4021 cacctgctcc gatgttaaat agcgttttac tgatatcagc ctgaagggta gggtcgccac
     4081 tgcttgcagg atgtgttttg ctgagttcac gcgcgatgtt ttttccggtg aaggatactc
     4141 caccgcttat tgtaccattg gcaactttaa aatgccgggc ataatttagt gaaacaatat
     4201 tttgaatatc gacgccgaaa ttatcgtgca tgagttgaat gcctgctttg tttttatcaa
     4261 aggaatattc tccaataata tttgctgtag ttgcattgtc gattgcaccg ctgaaggtat
     4321 tgtaataatg agcgtgtatt aatgcatctg aatgataagc ggtgagtgca ggatttattg
     4381 aagatggggt gatggcatgt tcggaaaaca caggcaattg cccatgtaaa aaagcgctta
     4441 ttattgtgca tatgctcaat gtaattattc gcttcatctt gccacagtta atgatccggt
     4501 atatgttttt ttattatccg tttggttcac cacgaacaaa taaatgtaag tgccatcggg
     4561 taaaggccct ttatcaatgt tccacgaggc atcccagtta ttatcataac catcggcttc
     4621 gtaaacgatt tttccatatc gattataaac agtgagatgg ttatcaggga aatatgctaa
     4681 acaactgatt tcccacttat cattgatgcc atcgccattt ggagtgatga catcaggaat
     4741 aacaggatct gcgcatgcgg tttgggcaat aacggtattt gttacaacgc ataatatcga
     4801 caccaaaaac aaaccgttca atttcatttg gctgtggttt tttcgtcgtg aatgattgct
     4861 atcggtctaa agccgatgaa tggtgatggc ccggaaattc ctgcataatc atcagggcca
     4921 tctatgcgaa tatttggttc agggctattc caacctccac ctttgtgctt tcctttttcg
     4981 agcagcattt cagttgtatt cccactcatg ttgtaaagtc cgtatcgatt agtggtatac
     5041 gccatcacag gagttaatgt gaggctgcat gtatcacctt ttttagcatt gcaacgataa
     5101 tatccttgtt tgctgatgta actatccttc ccccagccat aagtattatc agcctcgcca
     5161 ccacgcgctg ctattttcca ttctgtacta tcaggtaatc gcacttcaat attaaatccg
     5221 cgcagcgaat atgcttctgt gagccatttg caatacatca ccgcaccttc gtaagaaata
     5281 tttacaacag gataatgatc ataggcttcg tgctgaaaat agaattttga gtaggcactg
     5341 ctatcgccaa aaatagcatc ccactgtgct gtgtcgacac cggcaatacg caattcatcg
     5401 taacgacctt ttatttgcaa gtcattgagg aaatagcgat aattttcatt gctgatctca
     5461 taggcttgta tataaaaatc atggttcatg ccatctgcct gaccgggtat gtacataaat
     5521 ccgtgacgtt tcaaaaagcg gcctgtctta ccatgatgag gaggtgtgaa tcctgtagcc
     5581 aaaacagcca caacacataa tgctgatatc catgcaattt tcggcatagg gtataaatat
     5641 actataaaat atgaacgcac atacaacggg gtatgtgcgt tcgagtcaat tagattcctt
     5701 gattgcagct attacttcac caagcttgta acagtaacta ctgtgcggaa gcctatgaat
     5761 ggtgaagctt cagttgaata cgggaactcc tgggcggcgt ctatgcgcaa atattcgggt
     5821 tcgctccacc agttgccgcc tttggtttct ccgtctgtgc ttatccattc actaacgttg
     5881 ccactcatat tataaagatc gaatccgttt gggaaatagg attttgaaat ggctgtcatg
     5941 taagcgccgt cttccgacaa tgaagaggtt ttataatttg ccagataaat acctttttta
     6001 ttacgcaatt catttccttc ccatgggtaa atactacctt gaataccacc atgagcagcg
     6061 tatacccatt ctgctttttc gggcaagcgc actttagcac ctacgccatt ggcttttaat
     6121 tgttcttcca gccaggtgca atacaatctt gctgcttgtt gggaaatatt tactaccgga
     6181 taattgtcaa aagcaggatg tgtaaaatag tgctctttca tttcttctaa tgaagaatag
     6241 ggcaatatcc atttttctga ttgaacgttg gcgacttcat attcagcagt ctttccttta
     6301 gccttcaggt cttcaagaaa ggtacggtat tctttattgg taacttctcc tgcctgcatg
     6361 taaaatccgt ttatggtcag atttatacca tcaacagttg tggcacctga aggtacgaaa
     6421 acgaagccct gttttttcat cacctttgcc tgcttttgtg gggaaaagga agtggttaat
     6481 gtaatgaggc agagaatgag tagcgtagag gtcaagtatt tcatcacaag tttttttata
     6541 taacgttttc gcatagctaa gtcgtataaa aaagaaaacc cccccagatt tacatcagag
     6601 gtttagtatt gaatatatca tattaggcag acacaccggc tttcggagcg gctgccataa
     6661 tttcagcatt tgctttatcg gcatactttt caaaattctt cacgaattga gcggcgagat
     6721 tatttgcttt tgcatcataa gcttgcttat cgctccaggt attgcgcgga tcgagaactt
     6781 ccgtaggtac gtttggacag gtggttggga aatgcaatcc gaaaacatcg tgtgttgcat
     6841 aagaaacgct gtccagttcg ccattcagtg cggcagtgat catggcacgt gtataggata
     6901 atttcatacg tttaccggtg ccatatgcgc cgcctgtcca tccggtattg atcaaccaga
     6961 tattgacatt gtgtttgcgc attttttcac cgagcagttc agcatattta gttgggtgca
     7021 atggtaaaaa aggcgctccg aaacatgtgc tgaaagtaag ctgtggttct gttactcccg
     7081 cttctgttcc ggctactttt gcagtgtatc ccgacaggaa ttgatacatg gcctgtcccg
     7141 gtgtaagttt gctgatcgga ggcaacactc caaaggcatc gcaggtgagg aagaagatat
     7201 tttttggtaa gccgccttta cttggcacca tgatattatc gatgaagtgg atagggtagc
     7261 ttacgcgagt attttccgtt ttggtaatat tggtaaaatc tacagtacgt gttccgggaa
     7321 tgaattcgac attctcgagt atagcaccga atttgattgc attgaatatc tgaggttcat
     7381 tctcgcgcgt caggttaaca catttagcat agcaaccgcc ttcgaaatta aatacgcctt
     7441 cttcagtcca gccatgttca tcatcaccaa tcaggcggcg atcagggtca gcgctgagtg
     7501 ttgtttttcc ggttcctgat aaaccgaaga acacagctac atcgccttct tttccgacgt
     7561 tagcagagca atgcatgctc aatacgcctt tttcctgtgg cagccagtag ttaaggacgg
     7621 tgaaaatacc ttttttcatc tcgccggtat agcctgttcc accaatcagg atgatgcgtt
     7681 tggtgaaatc gatcatggcg aagttcttat cgcgggtatt atccacagcc ggatctgcct
     7741 ggaagcccgg ggcattgatg atgagccagt cggggttcat ggttttgatc tcttctgctg
     7801 ttgggcgcat aaacatgtta tcgcagaaca tatttcccca agggtactca ttgatcacac
     7861 ggatattgat gcggtattta gggtcggcgc aagcgtaatt gtcgcgcacc caaatgtctt
     7921 tatcgctcaa ataagccagt accttggcat acagcttatc gaaagtggca ttatcggttg
     7981 ggatgttgaa gttgttccag tcaaccgtat tggtagtttt ctcatccttt acggtgaact
     8041 tgtcttttgg cgctcttccg cgaaatttac ctgtatcaac tacgagtgcg ccactatcgg
     8101 ccattacacc catgccacgt tgaagggtat gctccaccag ttcggcaggg ctgagattcc
     8161 agaaggcgtg accggtgtga ttcaagccta tagccgcaag ggacgctgcg gggttcttta
     8221 ttcctgattc catctttata ctttaagggt tgtttcgggg gcaaagatac ggaggcaggc
     8281 aggaaaaaag ggtaacattt gtcaacgctc acgaaaaaga ccgcatgcct cttaagtgct
     8341 gtcataaagg gtgttgtttg tttatgatta ttttttgtcc attatgtatc attctgccaa
     8401 ttcaaatgca taatttttct tttccgatac tgaacattgg aaattatgta ctttagcgct
     8461 agataaaaca catgcacaag agtcctcgaa ttctgctttc atttgctttt tttctgatgg
     8521 tttccggtgt ttatgcccag gcattttcca cactgaaatt gacctcgctc cacaattatg
     8581 aaatagtgga tgatgcgttc aaccgcagcc tggttggctt atcccctgaa atagggtatg
     8641 aagcttacaa ctactcatat caattggaaa tagcggcata tataccgcga cagatagatt
     8701 ctatggttta tgtatatgac agtttattcg ggtatggatt tgatgcccca gttagtgaga
     8761 ccatgacgat tttcactatt gggcttatgt caaggtacta tttgttttcc gattggatgc
     8821 ctgtcaatag gttatataca gtctcaggta taatgggtgt atttgaacat gtgaaccaag
     8881 attttattgc aggcggactg agtgaaggaa aaaaatttac aaataaaagc ctggacctca
     8941 cttttggctt ggggtttgaa acgcttatag atgattacct tatgcccttt atagagacat
     9001 cagtctattt ccctgtagca caaggcgaaa atccctataa tactgtttat gatattactg
     9061 agggatacgg tgtaagaata gccggcggcg tgaagttgta cctcagtgat atgtaattga
     9121 tacaagataa ggtatcaaag atatttttgg gatgcaaata agttagaaac gattttcaat
     9181 ctcttttaca tctgaattgc cactctttcg aggcgatatc tttttcccgg tagggcggat
     9241 attaatcctt taaattccat actcaacaaa acacctgcca gattacccgg tgtgagttgc
     9301 gatttcgatt gtatgtcgtc tatatataat tcgccgtact cctgtaaaat ggtgtgtatc
     9361 tgttgttcat cgtcatttaa gtcatgaaat aattgttgtt gtacatgttg cgtttttgtc
     9421 gtatttgaac tccaccccat tatatcaagt acatcttcag ttgattctac catcgccgct
     9481 ttatttgatt tgattaattt attgcagccg gttgagtatg gatgatgaat ctgtccaggg
     9541 aaagccatta catcgcgatg ataatcatct gcaagcagcg ctgtgattac ggcaccacca
     9601 ttgattcccg tttctacaac aataagcccg tctatcattc cggcaactat cctgtttctt
     9661 cgcgggaagt tttgacgatc cggattagta tcactcatga attcagttag caatccaccg
     9721 tttgtcatca ttttatttgc tgtttcccga tgcgcaggcg gataaatgcg atctaatcct
     9781 gttgccatta ctcctatggt aggcacatta ttttttacac atgccttatg cattgcaatg
     9841 tcaatgccat atgccaaacc actgacaatc aaaatatttt catctttcaa tgatgctgca
     9901 aggtcttcgc aaattccttt tccataactt gttgcatttc gcgtacccac aaccccaagt
     9961 atgcgcgggg cattcaaatc ggcattgcct cgactaaata ataaaagtgg tgcatcagga
    10021 cattgtttta atcgccgcgg ataaagttca tcgaggtatg tcaaacattg aacctcattt
    10081 ttttctatga aacgcagttc ttgttcaacg cggtcgaaat cggaaaaccg gcgaatatta
    10141 tctgcaacgg cttcaccaat gcccggtacc ttcaatagat gactgcgctt gactttaaat
    10201 acagcttctg cactgccaca ataactcacc agattccttg ccagcaccgg cccaattccc
    10261 gggatgaggg atattgctat taaatgatac atgatttgta aatacaagac tatatttgtg
    10321 aagactataa ccgcaaatca aaagaaatca tgcgccatta ccaaaccatt ttcgttactt
    10381 ttttttgtat aacattctcg ctaaccgctt acggtcagca gattctaggc gataacttca
    10441 atcggcctgc ttatgattat cgcagcgaaa aaaatccata ttattggaaa aataaaaaac
    10501 ctttcgatgg gtactggcaa caggatgtgc actacaagat tgatgcgaaa gtggatgaaa
    10561 agactgatat tatttcggga aaggtcgaat taacgtatta taacaattcg cctgatgcac
    10621 tgccttttgt ttatttccat ttgtatcagg aagcctttca gccggattct tatgtcgaaa
    10681 cacttaatga agtgaataac gtaaaaccta cttatggaaa atatgaggct gaaaagaaag
    10741 gatgtgatgt aactgaaatc accgtcaccg caagaaatgg aaaaaaggtg agcgagtcaa
    10801 ctaaactcac tcaggacatt actgtgttga aggttgaatt aaatgaaccg gttttatcag
    10861 gtcagtccat aacctttagc attccattta atacctattt cgatagcgga agcctgcgca
    10921 gaagaatgaa aaaattcgac agtggtggct ttgaacatta tgacggtgtg cattggtatc
    10981 ctcgaatttg tgtgtatgac agaaagaaag gatgggatac cgatcaacat ttgggaaaag
    11041 aattttatgg cgactatggc agctacgaag taacacttga ttttgccagc aattatgtgg
    11101 ttgaaggaac aggatggctg gagaatgaag ctgaagtgat gcctaaatcg ctgcgagaga
    11161 aattggatat tcgcaatttc gcaggtaaac catgggagtc gcctgcatcg gttgttattc
    11221 cttatatcga tggcgataga aaggtttgga aatattatgc attgaatgtg cacgattttg
    11281 cttttacggc agatcctaat tatcgcattg gtgaaacaat ggctgtgatt aatggtcaaa
    11341 ctgttcgatg tatagcactt gctcaggaag gtcatgcagg cttctggcaa aatgctgcag
    11401 attacactaa acaaattatt gaaactttca gcgctgattt cggtccttat atctggccga
    11461 agattattgt tgcggatgca cgcgatggta tggaatatcc aatgctaaca ttggatggtg
    11521 gctataatcc tgcatattac gatttgctga tacacgaggt tggacacatg tggtattttg
    11581 gcatggtagg caataatgaa acctaccgcg catctatgga tgaaggtttc acacaatttc
    11641 tgacaggatg gggatatcgg aaaatatcag gggactatcg cattgatttt cctgagcaaa
    11701 ataaatacgt ggctaaatat cgaaatcctg ataaaataat gatgagcgaa gtctacgcag
    11761 cctatttgtc ggatgctgca cgtgaagctg atgctacact taatacacat tccgatatgt
    11821 ttaactcggc gctagcacac ggtggagggt atagacatgt gtatttcaag actgcggtta
    11881 tgttatacaa tcttcagtat gttttggggg atgaattgtt tttggcagcg atgagaaatt
    11941 ataccgatga ctggaagctt tgtcatccgt atccggaaga ttttagaaat tctatttcgc
    12001 agtatacgca cactgattta gattggttct tcgatcaatg gctggagaca acgaaaaata
    12061 ttgactatgg gattaagaag gtggataaag tgtcttcaac caatgtagat ggacaaactt
    12121 tgttgaatta tgaaataacc ttagatcgca agggaagtat gcagatgccg atagatttct
    12181 ccgtggtttt ggataatggt gatacactcc gctactatat tcctaatacc tggtttaata
    12241 aatacgagta tcaaaaaccc tcgcaaggtc gtcttgatag aacttatttc caaaatgtga
    12301 tctcattgcc taaatggtat ggctgggata aattgaatga tacctacgta gcatccatca
    12361 ctactcccca taagataaaa gatgttatca tagacccgag tgatcgatta gccgatatta
    12421 ataaattaga taattcatgg aaatgcccga cagaatgggt ttttgattct caaatcagaa
    12481 atgctcctga ttgggatcat tacactatgc gctggcgccc tgaaatctgg ggtaatgcct
    12541 acgacggact aaaagtcggt ttgcacttga atggcgatta ctttgcatat aaacacaaat
    12601 tgtcgctaac tgcctggtat aatacaggtt tggcacaagg tgcattgtat gacaatgaat
    12661 ttctaggaaa tacacttgac aatgccaatg aagatatcag cgataaattt gacaaattta
    12721 atttcgattt caactatcgc acgtctacag atagattcct gcctttatca gatttcattc
    12781 tgaatacgaa gtatctcgat ggtgtatttg tttttgaaac cggatttgag aaaaaactgg
    12841 gagagaataa tcgagacctt ttaaaagtgt atgtaaaaga catgcaattt aacaatattg
    12901 aatatttgta tggtccttat gtgattgagg gtaacaaaaa caatactgta aatatcgatt
    12961 acacacatcg atataattac tttaccggca atggaacaat caatgttcgc ttccgcagta
    13021 atgatttgtt gagtgattat gatttttctc gtttgcagtt tgaagccatt aatattacaa
    13081 aacttggtaa gttagattta cacaccagat attttgctca atggggctat ggcagcaatg
    13141 tgcctttcga atctaaatta atgttaggtg gggcaaatat tgaagatatg ctcgatagta
    13201 agtttacgcg atctcgtggt ttcttccctg atgaatgggg cgaattcggc actacaacca
    13261 atcatttgca aatgggtggc ggacttaacc tgagaggtta ttcaggctat tttgctgccg
    13321 atgacattga tggtgaaact caattggcgt atgcaggact aagcggaact tctttaagtg
    13381 ctgaattgca atttgaccgc atcgtgaaaa aacaattgat cagatttatt aaaatggaac
    13441 cttacctgtt tgctgatgcc ggaatcatgg ttggcgataa tgcattcgag gaaatatcca
    13501 agccaagaat agatgcgggt atcggatcag catttacgtg gagttggtgg ggaccattgg
    13561 aaacggtaaa accattaaca gtgcgcatag atttaccgct gttcctgaat cgtccgcctg
    13621 ctgaagatcc ggaatatgtg aaattcagat atgttgttgg aattaataag acattttaat
    13681 tgattcccat caaaataaat aagcccgaac aattgattcg ggcttatgta ttttatagca
    13741 aagttgttaa tccattggca gatacaacaa ccatttctca ataaaataag cttccggtat
    13801 ccattccgtg attgggaatt tgtaaaggat atctttgaaa ggtgcaattt caagatctag
    13861 aaaaccaccc ttgagcatga taagtccatt cggtacatca tttacagaat gtccacgtcg
    13921 aataagattg atagtccaat cgtacaatat catcatgtca gccaccgcgc gtgtaactac
    13981 aaaatcatat ttttctcgga tatcttctgc acgaacctgg tctactgtga cattgcgcaa
    14041 tccgagtgct ttgtaaattg attccaccac tttcactttt ttgtcgatag aatcaatcag
    14101 atggaattct acttccggaa acataatggc taaaggaatt ccgggaaaac ctcctccggt
    14161 gccgaggtct aacacttttg tgccgggttc aaaagaaatg aattttgcaa tcgcaaggga
    14221 gtgtaaaaca tgatgctgat aaatattatc aatatcctgc cgagagatca cattcacctt
    14281 ttcgttccag aatttatact gggcaccgat ttgtttgaat tggctgatct gtaggggact
    14341 gagttcaggg aaatatctga ggatatgctc cacggaaaca agtatagcca tattttccga
    14401 aattttcaaa ataacgatgt tagtgacagg aatattacca ctaaaaaatc tattatcttt
    14461 tcagcaaaat tctagctatg gcaattcgaa tggttccaga cgataatcaa ccccaacaac
    14521 agcgaaaact gcctggaaca ggtggcagag ggggttccgg aggtggtggc ggcaatctga
    14581 taaccatgtt gttgccgcta ctgctgggta tattccgcaa aaaccctaaa accggatgtg
    14641 ttaccttgat agtgatagcc gcagtaattt atttcatggg cggcaatctt ggaggtctgc
    14701 ttaacccagg aggtggcggc actaatatca ctgatctctt caatacgggt gcttccttcg
    14761 accccgttaa gtatgatgca acagaaatat ttgaaccgcc aattaccgat aatgccaaga
    14821 atccattgcc ggatgcagta tctctgctta gatatgcacc aacgcgtcgt aatcaaggct
    14881 cacaaggcag ttgcgtaggg tggggaagtg cctatgctgc gcgtaccatc ctcgagtcgg
    14941 aacgaacagg ccgcaaccct gatgatattg cgttcagccc tgcttacctc tacaaccaaa
    15001 ttggtctgga aggttgtcag ggagcttaca tcaataacgc gatggaggta atgaaggatc
    15061 agggattggt aaccctgcgt gaatttccgt acgacgacaa taattgcact aaacaaccta
    15121 catcatcaca aaaacagcag gcttctcagt atcggatgaa tggcttcaac aggcttactg
    15181 ttggcgacgc atcaggtacc ggtaatgaaa aaattgacct tgttgcaata aaacaaaatc
    15241 tggcacaagg tgcacctgtc gtgatcggta tgatggtcgg aggctctttt atgcaaagta
    15301 tgatggggca agaagtttgg catccaacag aacaggatct ttccatgtat ggatttggag
    15361 gccattgtat gtgtgtgatc ggatatgacg actataagga aggcggagca tttcaaatca
    15421 tgaatagctg gggtaaggaa tggggcgata atggtgttgc ttgggtgcct tataatgtgt
    15481 tcgaacactt tacagtggaa gcgtatggcg tctatcctat gggttcagca gataaaccac
    15541 ttggcaacga tctcaatgcc tcattcggtt tggtgatgaa tgataatggt cgacatattc
    15601 ctctggtaaa ggatggcgaa aatacattct ctacgcaatc gcccgtgaag aaaggaacga
    15661 aatttaaaat cgaaataacg aattccaaag aatgctatac ttacgtattt gctttggatg
    15721 agaataaaaa aagcaccgta cttttcccat acacgccaaa gcattcccct tattgcggta
    15781 tcaccggaac cagacttttt ccgaaggatt acagtatgga attagatgca gttggcagtc
    15841 gcgatgaaat ggcaatagta ttgtcaaaaa caccattgga ttatcaagcc ctaaataatg
    15901 ctgcaaatgc ggctgccgga aattattccg caaaaatgac acaggcatta aaaggaaaat
    15961 tgcaatcaga ggtgagcttt tcagccggtg atgtcattgg cttaaaaact gtgcttgctg
    16021 agaatcaagc ggtggtgatg gtgattggta tcgacaagcg ataaacatat cctgaaatca
    16081 catcatggat tatatttttt aattttattc catgacaacc caatggcgcc taacagcgat
    16141 gtgcttgctg tgtactgtgg tgatgcaaat aaatgcccag gacacctata ttcccttcag
    16201 aacttcattg cctttttcct tagatggagc attagatgag gccgactggc agcgcactcc
    16261 ggtagaaacg gctttcatgc aacaaggtcc ggttgccggg gctacaccaa cagaaaatac
    16321 tgaggtacgg atcatgtatg ataatgatta tttgtacgtt ggcattacct gtttcgactc
    16381 agagcccgat aaattaattc gcacagaact tgaacgtgat ttcccaatcg gaaatgaaga
    16441 cggcacaggc gtgactatag atacttatca tgataaatta acaggcatgg attttctttc
    16501 caataccctc gatgccagat gggatgcaca ggttacacaa gatggtggcg gtttgtcaga
    16561 atcgtttaat actttctggg atgctaaaac taaattatat gattttggat atatcactga
    16621 atatcgcatt cctttttctt ctcttcgatt tgaggaaaca gagcaggtga caatgggtat
    16681 tcgggttgcc agattaatca aaagaaaaaa tgaactcatc acctggcctc catgtgac
//
LOCUS       DAOQOU010000053        16547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1752_length_16547_cov_5.734659, whole genome shotgun sequence.
ACCESSION   DAOQOU010000053 DAOQOU010000000
VERSION     DAOQOU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16547
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_16547_cov_5.734659"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLM90_06465"
     CDS             complement(<1..1216)
                     /locus_tag="PLM90_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ89049.1"
                     /translation="MSTEQNNQNKTKPNGNRKFNFYWIYAIIAVILISFQFFPFQGSP
                     VVLKDISTFKDDMLLKGDVNRIVVVNKEYAEIFIKEDSLKKKIYTDDKVPEKGAQYKW
                     IIGDTETFERQLTEWQEGKTSQPVTIMYETRHNILVSLLSWILPFVIILVIWILVMRR
                     VTGGSGMGGGGQIFNIGKSKPQMYDKDTKVNVTFNDVAGLDEAKEEVMEVVDFLKNPK
                     KYTSLGGKIPKGVLLVGPPGTGKTLLAKAMAGEAQVPFFSISGSDFVEMFVGVGASRV
                     RDLFRQAREKAPCVIFIDEIDAIGRARGKNMIQSNDERENTLNQLLVEMDGFGTDKGV
                     IILAATNRPDVLDPALLRPGRFDRQISIDKPDLAGREQIFNVHLKPIKVSPEVDAHKL
                     AVQTPGFAGADIA"
     gene            complement(1235..1609)
                     /gene="rsfS"
                     /locus_tag="PLM90_06470"
     CDS             complement(1235..1609)
                     /gene="rsfS"
                     /locus_tag="PLM90_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMZ89050.1"
                     /translation="MRKSKKQALPLQYSLTELVIDSILDKKGEEVVHLDLTALPDAVS
                     DHFIICHADSTTQVRAIGNHILENVFEKSGDRPFSKEGLDNAEWIIIDYMDVVVHVFY
                     KEKRYFYQLEDLWHDAVMKKVS"
     gene            1645..2397
                     /locus_tag="PLM90_06475"
     CDS             1645..2397
                     /locus_tag="PLM90_06475"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMZ89051.1"
                     /translation="MSHTLFIGKRHIRLASADSTNNWLKGYLEVHPTAPEGLAVTASD
                     QTNGRGQSGKRWESAPGLNLAVSYLLRPTFLAPAGMFTLNKAIALAVRDTVAEFLPET
                     RIKWPNDIFSGNHKVAGILIESSVNSRVQWVVAGIGINVNQMKFGPEAPRAGSIAAIS
                     GHKIHIDDLLQKLHIHLEHHYLRLRAGHDADMHQAYLDHLMAYQQSFRFIHDNQSFQG
                     VLTGVDPEGRMIVDMNGKITSFAMGEITWEFD"
     gene            2480..3385
                     /locus_tag="PLM90_06480"
     CDS             2480..3385
                     /locus_tag="PLM90_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HMZ89052.1"
                     /translation="MRHLILILVSCITITMQAQLSQQNYIVYNTRTKQIENLDSIVAE
                     MQHVDVLFFGEEHNDSVGHYLEATLFEMMHKSYGAKTALSMEMFDRDVQPVMDEYLAG
                     LIKEKQFVKDARVWSNYRDYKPMVEYAKTNNLHVVCANAAGRYSSLAVRKGMQGLMAL
                     PEASKDHIAPLPFDTASGPYFDKLVDVMSGHDTTAGAAAVTAPGMDMVMGQSTWDATM
                     AYSIAQYLKAHKGDKVFQVNGRFHSDEHYAVVTQLKNYRPKATSLVISSGSDENFPNI
                     NWDDHKADGDYIIITDPKVPKTFEE"
     gene            3649..4761
                     /locus_tag="PLM90_06485"
     CDS             3649..4761
                     /locus_tag="PLM90_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89053.1"
                     /translation="MRFMLTLRNGVAGACPVPTNMHVPKLPAKWLMMVCVIALSTSHT
                     KAQTTLLYTINYVADRTTMYVYPDSLQSYLIHEQIALLPVYQRDSVIRMVYTNKDVKN
                     ITYYKADSRFEDWMTRPAKSIVDKKSVKLYNSSGMLMLTQTHSADYKALYSDLKTQMS
                     TLGTDLIPDYVQLTNTLKSNLLANGFVMTNLGGGTYRFVKDSVEVRYNNSRKMNEMRL
                     YHADGSLKYALLRDFAVNSFGQTTPVLTQERRPDTRFPGGCVEEVVTTKYPTYYMVYG
                     GTGKIEEDDMAEDELYISPNPATTETTISYPPEQTDNATAMVEVFDLTGKMVIQKYVN
                     ASEGSVVINTSSLEAGVYAVIYTCALLKLESQFVKQ"
     gene            4773..6305
                     /locus_tag="PLM90_06490"
     CDS             4773..6305
                     /locus_tag="PLM90_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89054.1"
                     /translation="MKKSYLLFMLMVFVVAANAQVTPSCPTDVKGAEITDKTLVEFTP
                     TLLTDETWGTPTLLTDRRVFFVHGLGGEGDEAGTTAISWMQASLWSEQQYMINSARPD
                     YNNVSLEAAAMELKGDLESFADANSDAILIAHSQGGIVSRRVDKMYTTGEFGFEPRTF
                     GGMVTFGTPHQGAKILNNIEDLQDWVGESCEALSAGPVAEAVESSFFLDLFLDPVDYE
                     SFTEEFCNSVQNQILPLVMDDYLAGTTSAYSVGAPYLATLNTFVPEIPYVCFYGVETQ
                     PIFWHTLVHLFPGREPNNTITYGEAPFGATNDETAVAFADAMIGKYYGKYTSYNALYE
                     EYNDLLTGFDMATIGCYLSVFCAVDAIIQRDEAAIIRDAYEKGYDWLINANAAWEGFI
                     GMSELVEDGTTCTCIDWGPLGDLEEDIYPAGPDGSCATEDEDTNCYTTTNYIWQTHDS
                     DGIVVKESAAECLGQVSEGMVFTQMPGSNHLSMRNDANTKLKLIHLYDGVHGSFYKTN
                     PR"
     gene            6309..7565
                     /locus_tag="PLM90_06495"
     CDS             6309..7565
                     /locus_tag="PLM90_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HMZ89055.1"
                     /translation="MKICYCFISAVLLLCSCKEEELLTWDPYEYPEDPEPPNELIWQV
                     PLHQDTLGCLATWPQIYQNAILCSGEEGWENPVRVFAVDTSGNLMWESNAVFNENCSS
                     ISASVGTGAYQYQNMVAMLCNADPRVVNIQTGEVMWHYEVPGSGNHSPYMSGFGDQIF
                     HAYADGPNPYTKSYIARANIYTGIWDTVFSIEMVDGYSADLYPPGAWVDNNGDTIICF
                     QNRQWRNPPYDGKVDLYAYNLTADSLLWMYSDIDPDGNSSVFPPVYADGKVFFRGYFH
                     VYGLDAKTGNLIWTWASPNVANDLLYTNTLVVDDKVLVKTTGPELFALDPDNGSEIWH
                     TSGFGEIPSELVAFDGMLYFSTEIDGKLNAVNIQNGSLEWTMTSPNHGQPTSSWAYFS
                     RPVTIDPLLHRLYLTDFFYLLCIQLK"
     gene            7573..8307
                     /locus_tag="PLM90_06500"
     CDS             7573..8307
                     /locus_tag="PLM90_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89056.1"
                     /translation="MRPATCLIIAISLMQHSFAQIDDVKPVNGHQRIDVNLDLGYRIE
                     RTGSNLADFVHTTGFGAGGDYSIGSASLRLVMSPYIHAQSGNDFNEMKAYYKAKGYTL
                     NSFNGGMYYSTGMLLGARYVIPTRRPSGLPSFYAALQYGFQAAHTPACDINYSTINGD
                     IISIKDDQAIALGPTWRLQLGADVLRSGDALYSIQAGVRQERAALPVDRTTLGVPGLD
                     TPGSTEIFQWNGIYAVIGLGVAFNME"
     gene            complement(8342..9295)
                     /gene="metF"
                     /locus_tag="PLM90_06505"
     CDS             complement(8342..9295)
                     /gene="metF"
                     /locus_tag="PLM90_06505"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HMZ89057.1"
                     /translation="MKVTEHLNRADKTLLSFEILPPLKGKSIQNIYSVLDPLVEFKPA
                     FINVTYHRSEYIYKKRGDERFDKVFIRKRPGTVGICAAVQFKYHIDSVAHIICGGFTK
                     EETEDALIDLSYLGIDNVLLLRGDASKNERDFIPEPGGHSHAVDLVRQVVDMNRGKYL
                     EEDSIQGYKTDFNIGVAGYPEKHFESPNLKSDLKHLKEKIDAGADYIVTQMFFDNQQF
                     FAFVDRCRAEGITVPIIPGIKSITAASQIHSIPRTFHVDIPEALIDTIEKCTTAEAVK
                     KAGVAWCIEQSRELVKAGVPCIHYYTMGNVDAMRSILTEVF"
     gene            9510..10781
                     /gene="purD"
                     /locus_tag="PLM90_06510"
     CDS             9510..10781
                     /gene="purD"
                     /locus_tag="PLM90_06510"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMZ89058.1"
                     /translation="MNILLLGSGGREHAMAWKIAQSKQCDQLFIAPGNPGTASCGKNI
                     AVKATDFPALATVCLENKIDMVVVGPEDPLVAGVVDYFASNPAIAHIPVIGPNKQAAQ
                     LEGSKAFAKAFMIRNKVQTPTYKTFTASDSAAAHEYLKGHPMPVVLKADGLAAGKGVV
                     ICQTAEEAIVEFDQMVSGKFGVAGNTVVVEQFLTGIELTVIILTDGKTYTILPPSKDY
                     KRVGDGDTGLNTGGMGAVSPPPFATEAFLDKVKEKIIDPTMMGLKNEGIHYTGFLYFG
                     LFNSNGEPYVIEYNCRMGDPETQVIMPRIANDIIDMYKAVAQGTLASQPLITDPRPCT
                     TVILASHGYPGVFEKGYAITGIENTEQCMVFQAGTALNNNGELVTAGGRVMAVSAYGD
                     DIASALRTSYINAGRIYYESRYFRRDIGWDM"
     gene            10992..11417
                     /locus_tag="PLM90_06515"
     CDS             10992..11417
                     /locus_tag="PLM90_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89059.1"
                     /translation="MKKLMTMGLVAGIVATLASCSDDSLNGITTAEAESKSPGSWKVS
                     YYFDNSDGVSHELDAYTFEIATDGSLTVSGGGATYTGTWVVKNSDDDPNYDTEIDFSI
                     MGNSDMDKIDGSWLMGTINDTKMELLDDSGSEELHLSRQ"
     gene            11641..12063
                     /locus_tag="PLM90_06520"
     CDS             11641..12063
                     /locus_tag="PLM90_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89060.1"
                     /translation="MRTSSFLIAGVATAMILLSACTKTDTFILSVSDAENIIPGSYKV
                     ATFIDIAGEGTVYENYIFQFTSNGDLTATDGTDTYTGTWSIENENDPVYDKKVTISIG
                     GNTEMDVLNHGWLVEEVTDATLYLTDDTGVESVHFVKN"
     gene            12257..12841
                     /locus_tag="PLM90_06525"
     CDS             12257..12841
                     /locus_tag="PLM90_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ89061.1"
                     /translation="MPVKKIDRGELIKQALNLFRTQGYHKTTMSEIGTACGLLKGSIY
                     HYFSSKEDLMREVLEVLRNYYRDKVFIHAFNDALTAEQRLNMLGEKSIEVFVNGDGAC
                     LMANIAMETNDVVPEFRAPIRAFFDDWTNALTHIYSDKYSKEAATIFAGETICAIEGA
                     AMLMRIYNDRTYLDKAHQMITEKFKVGSKAALLK"
     gene            12908..15295
                     /locus_tag="PLM90_06530"
     CDS             12908..15295
                     /locus_tag="PLM90_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMZ89062.1"
                     /translation="MNRIIKKIAVLGSGVMGSRIACHFANIGIEVLLLDIVPLQATEE
                     QLKQPAFRNKIVNDSLQAAIKQNPSPVYSQKVLKRITTGNFDDDMERIADCDWVMEVV
                     VENLTIKQQLFEKVDKHRKQGTLVTSNTSGIPIALMSEGRSEDFRRHFCGTHFFNPPR
                     YLRLLEIIPGPDTEKSVIDFLMNYGDRYLGKTTVLCKDTPAFIANRVGVFGMMAVLKV
                     MEEMQFSIAQVEALTGPIYGRPKSATFRTADVVGIDTFVKVAQNTYAACPDDESRDIF
                     QIPAYINTLVENKWLGDKSGQGFFKKVKNAQGQSEILTLDLNTLEYQPSTKAKFAALD
                     AAKPIDNLKDRIRTLHATGDKASEFYNKLNALVFKYVSHRIPEIADELYKIDDAMRAG
                     FGWELGPFEIWDVLGVEKFVAWMEQHGTTPASWVKEMLQKGYKSFYLVENGKRKYYDA
                     RSGGYVLIPGMDAFIILDNYRAQKPVWQNSGATLHDIGDGILNLEFHTKMNVVGSEIL
                     EGIQKSIDIAEKNYRGLVIGNDAANFSAGANIAMMLMLAIEQEFDELDMAIRVFQNTV
                     ARIRFSGIPVAVAPHGLTLGGGCEMTMHADVAVAAAETYIGLVEVGAGLIPAGGGTKE
                     LALRASEKYFKGDIEIPTLQEMSLNIAMAKVATSAREAFEFGLLRNGIDKEVINGARL
                     IAEAKKQVLSLADAGYVQPKPKSDIRVLGRSALGSFYVGISSMRMSGYISEYDEKIAK
                     KVAYVISGGDLSMPTEVNEQYLLDLEREAFLSLLGERKTLERMQHILKTGKPLRN"
     gene            15318..16493
                     /locus_tag="PLM90_06535"
     CDS             15318..16493
                     /locus_tag="PLM90_06535"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMZ89063.1"
                     /translation="MTEAYIVAGLRSPVGKAPRGMFRFMRPDDIAVQVITQLLAQIPQ
                     LEKSRIEDCIVGNAVPEAEQGLQIGRQIAMRALDKSTAGITINRYCASGVEAIAIATA
                     KIRSGMADCIIAGGTESMSLVPTAGWKTSLNYEVAAEHPEYLIGMGLTAENVAKEFNI
                     SREDADAYAFHSHRKATEAIDKGFFKTGIAPIAVKEIYVNEKNKRAERNITVDTDEGP
                     RRDTTVEALAKLKPVFAAKGVVTAGNSSQTSDGAAFVIVMSEKMVKELNLQPIARMVS
                     CASVGVEPHIMGIGPVAAIPKALKQAGMSLSAIDLIELNEAFACQALAVIREAGLNPD
                     IVNINGGAVALGHPLGCTGTKLTVQIIHDLKRLNKKYGMITACVGGGQGIAGVVEVL"
BASE COUNT         4908 a         3463 c         3633 g         4543 t
ORIGIN      
        1 tcgcaatatc agcaccggca aatcccggtg tctgcacagc caatttatgc gcatctactt
       61 caggcgaaac tttgatcggt ttcaaatgca cattgaatat ctgttcacga cctgcaagat
      121 ccggtttatc aatagatatc tgacggtcaa atcgacccgg acgcaataat gcagggtcta
      181 atacatccgg acggttggta gctgccagaa tgattacacc cttatcagtg ccgaatccat
      241 ccatttccac caataactgg ttcaatgtat tttcacgttc gtcgttgctt tgtatcatgt
      301 ttttaccacg cgcacgacca atggcatcga tttcatcaat aaagatcaca caaggtgctt
      361 tctcacgtgc ctgacggaaa aggtcgcgca cacggctcgc acctacaccc acaaacatct
      421 ctacgaaatc ggaacctgaa atactgaaga atggtacctg cgcttctcca gccatcgcct
      481 ttgcaagcaa tgttttacct gttcccggag gacctaccag taacacacct ttaggaattt
      541 tacccccaag tgaagtatat tttttcgggt ttttcaggaa gtctacaacc tccatcacct
      601 cttccttcgc ctcatccaat cctgccacat cattaaaggt gacattcact ttcgtgtcct
      661 tgtcatacat ctgcggtttc gacttgccga tattaaatat ctgacctcca ccgcccattc
      721 ccgagccgcc ggttaccctg cgcatcacaa gaatccaaat cacaagtatg atcacgaacg
      781 gtaatatcca actcaataaa ctcaccaaaa tattatggcg tgtttcatac atgatagtaa
      841 caggctgact ggttttgcct tcctgccatt cagttagttg acgctcgaat gtttcagtat
      901 caccgatgat ccatttatac tgtgcacctt tttcaggaac cttatcgtcg gtgtatattt
      961 ttttcttaag actgtcttct ttgataaaga tttcggcata ttccttattc accactacaa
     1021 tgcggtttac gtcacccttc aataacatgt cgtctttgaa agtgctgata tctttcaaca
     1081 cgacagggga gccttgaaaa gggaaaaact ggaaactgat aagaatcaca gcaatgatcg
     1141 cgtatatcca ataaaaattg aatttgcgat tgccattcgg ttttgtcttg ttttgattat
     1201 tctgttcggt actcattgaa tagtatatac ggtattaact cacttttttc atcacggcat
     1261 catgccacag gtcctcgagt tgatagaagt aacgcttctc tttataaaaa acatgcacta
     1321 caacatccat atagtcgata atgatccatt cggcgttgtc gagtccctct ttgctgaacg
     1381 ggcggtcacc tgacttctca aacacatttt cgagaatatg gtttccaatt gcccgcacct
     1441 gcgtggtgga atcggcatga caaatgataa aatggtcact gacagcgtcc ggtagagcag
     1501 tcagatccaa atgaaccact tcttcccctt ttttatcaag gatactgtct atgacgagct
     1561 ctgttaagga atattgcaga ggcaatgcct gctttttcga ttttctcact tactttgtta
     1621 aaggttgcaa aattacgaat tcttttgtcc cataccctct ttataggaaa gcgccatatc
     1681 cgactggcat ctgccgacag caccaacaat tggctgaaag gctatcttga ggtgcatcct
     1741 acagctcccg agggtttggc cgtaaccgct tccgaccaaa ctaatggccg cggacagtca
     1801 ggaaaacgat gggaatcagc accgggattg aatctggcgg tcagctacct gctgcgacct
     1861 accttcctcg cccctgccgg tatgttcact cttaacaaag ccattgccct tgcggttcgt
     1921 gataccgtgg cagaattttt acccgaaaca cgcattaaat ggcctaacga catcttttca
     1981 ggtaaccaca aagtggcagg catactcata gaatcttctg tcaatagcag ggtgcaatgg
     2041 gtggtggccg gtataggcat taatgtaaat cagatgaaat ttggccccga agctcccagg
     2101 gcagggtcaa ttgccgctat cagcgggcat aaaatccata tcgacgatct tttgcaaaaa
     2161 ttgcatatcc acctcgaaca tcattatctg cgtttgcgcg ccggacacga tgctgatatg
     2221 catcaggctt atcttgatca tctgatggcc tatcagcaat cttttcgatt tatacatgat
     2281 aaccaaagtt ttcagggtgt attgacaggt gtggatccgg aaggacgaat gatagttgat
     2341 atgaatggaa aaatcacgtc atttgccatg ggcgaaatca cctgggaatt tgattgaaag
     2401 atgctaaggt gataataatt gcctttcttc aatgatttgt tgctcgctta ttctaatctt
     2461 cgtttcgtaa ttttacatca tgcgacattt gatactcatt ttggtatcct gcataactat
     2521 tacaatgcag gctcaactct ctcaacaaaa ttatattgtc tataatacca ggactaaaca
     2581 aattgaaaac ctggattcca tagttgccga aatgcagcat gttgatgtct tgttttttgg
     2641 cgaagaacac aacgactctg taggccatta tctcgaagca acattatttg agatgatgca
     2701 caaatcttac ggagcaaaaa ccgccttgtc gatggaaatg ttcgaccgcg atgtgcagcc
     2761 ggtgatggat gaatatcttg ccggattgat aaaagaaaaa caattcgtga aagatgcgcg
     2821 tgtgtggagc aattatcgcg actataaacc catggtggaa tatgccaaaa caaataattt
     2881 gcatgtagtg tgcgctaacg ccgccggcag atattcaagc ctcgccgtgc gaaaagggat
     2941 gcagggactc atggccttac ccgaagcaag caaagatcat attgcacccc ttcctttcga
     3001 cacagcaagc ggtccgtact ttgataagtt ggtggatgta atgagcggtc acgataccac
     3061 tgccggtgca gctgcagtta ccgcacccgg aatggatatg gtcatgggac aatcaacctg
     3121 ggatgcaact atggcttaca gtattgcaca atacctgaaa gcgcataagg gtgataaagt
     3181 atttcaggta aacggaagat tccacagcga tgagcattat gccgttgtta cacaattgaa
     3241 aaattaccgc cctaaagcca catcactggt catcagcagc ggcagcgatg aaaacttccc
     3301 gaatattaat tgggatgacc acaaggctga tggcgattat atcatcatca ccgaccctaa
     3361 ggtgccgaag acgtttgagg agtgatgatt tatttagtga tatcataaat gatatggcaa
     3421 ttcaaaggct gcatcatcat aattgtagga attaaaatat ccacaattca aatttcatta
     3481 aaccgcttac gtctcattta tatcaacttt ttgtttgatt acaattttcg ttgataaaat
     3541 caatctccgg ctaagcgctt acaaagtcat gaaaatattt tgagggaaat gaaaaacaat
     3601 ttattgttgt agcgataatt aattgtttaa cccttaaata attttcgtat gagatttatg
     3661 ctcacgctcc gaaatggagt tgccggtgca tgccctgttc caaccaacat gcatgtgccg
     3721 aagttacccg caaaatggtt gatgatggta tgtgtaattg cgctctccac atcgcatacc
     3781 aaagcacaaa ctaccttgct ttacactatt aattatgtgg ctgaccgcac tacaatgtac
     3841 gtgtaccctg attcgctgca gtcgtattta attcatgagc aaattgcact gctgcctgtt
     3901 taccaacgcg atagcgtaat tcgcatggta tacaccaaca aggatgtcaa aaacattact
     3961 tactacaaag cggatagtcg ctttgaagat tggatgacgc gaccggcaaa aagtattgtc
     4021 gacaagaaaa gcgtgaaact ttacaacagc agcggaatgc taatgctcac tcaaacgcat
     4081 agtgccgatt acaaagcatt gtattcagat ctgaaaacac aaatgtcgac actcggcacg
     4141 gaccttattc ccgattatgt gcaattgacg aacactttga aatcgaattt gctcgccaat
     4201 ggtttcgtga tgacgaatct tggtggtggt acgtatcgct ttgtaaaaga ttcggtggaa
     4261 gtgcggtata ataattctcg aaaaatgaat gaaatgcgat tgtatcatgc cgatggttcc
     4321 ttgaaatatg cgttgctgcg cgattttgcc gtgaattcat tcggccagac aacaccggtg
     4381 cttactcagg aacgtcgccc ggatacaaga tttccgggag gatgtgtaga agaagttgta
     4441 acaacaaaat accctactta ttatatggtt tacggaggaa caggaaaaat tgaagaggat
     4501 gatatggcgg aagatgaatt atatatttca ccaaatccgg ctacaacaga aactactatt
     4561 tcttacccgc cggagcaaac agataatgct accgcaatgg tagaagtatt cgacctcacc
     4621 ggaaaaatgg ttattcaaaa atatgtgaat gcctcggagg gttcagtagt aataaataca
     4681 tcttcactcg aagcaggtgt gtacgccgtg atatatacct gcgcattgct gaaactggaa
     4741 tctcaattcg taaaacaata attacaatta ttatgaaaaa gagctattta ctttttatgc
     4801 tgatggtgtt tgtagttgca gctaacgcac aagtaactcc atcgtgccct actgatgtta
     4861 agggggcaga aatcaccgat aaaactttag ttgaattcac cccaacatta cttacagatg
     4921 aaacatgggg aacaccaacc ttgcttacag acagaagggt gttttttgtg catggactgg
     4981 gtggtgaagg tgatgaagca ggaaccaccg caattagctg gatgcaggct tcattgtgga
     5041 gcgaacaaca atatatgatt aattctgccc gtccggatta taataatgtt tcactcgaag
     5101 cagctgctat ggaattaaag ggcgacctcg agtcttttgc tgatgcaaac tcagatgcta
     5161 ttttgatagc acatagtcag ggtggaatcg tatctcgcag ggtggataaa atgtacacca
     5221 ccggtgaatt cggatttgaa ccgcgcacct ttggtggcat ggtcactttc ggaactccgc
     5281 atcaaggagc gaaaattctg aataatatcg aagacctgca agattgggta ggggaatctt
     5341 gcgaggcact ttctgccgga cctgttgccg aagccgttga atcaagtttt ttcctagact
     5401 tgttcctgga tcctgtcgat tacgaaagct tcaccgaaga attctgtaac agtgtccaga
     5461 atcaaatcct gccgctggtt atggatgatt acctcgccgg aaccaccagc gcatactctg
     5521 ttggagcacc atatttagct acacttaata catttgttcc ggaaattcct tatgtttgtt
     5581 tttacggtgt cgaaacgcaa cccatcttct ggcacacact cgtacacctt tttccgggcc
     5641 gtgaacccaa taataccatc acgtatggtg aagcaccttt tggtgcaacc aacgatgaaa
     5701 cagctgtagc atttgccgat gcaatgattg gtaaatacta tggaaagtat acttcgtaca
     5761 atgcgttgta tgaagaatat aatgatctgt taacagggtt cgatatggcg acaataggat
     5821 gctatctttc tgttttttgt gccgttgatg caatcatcca gcgcgatgag gctgcgatta
     5881 ttcgcgatgc ctatgaaaaa ggatatgatt ggcttataaa tgctaatgca gcctgggaag
     5941 gatttattgg tatgagcgaa ctcgttgaag atggcacaac atgtacttgc atcgactggg
     6001 gcccgctcgg cgatctggag gaggatattt atcccgccgg tcctgatgga tcatgtgcaa
     6061 ccgaagatga agacactaat tgttacacca caacaaatta tatctggcaa acacacgaca
     6121 gcgatggtat tgtggtgaaa gaatcagcag cggaatgtct tggtcaggta agcgaaggca
     6181 tggtgtttac acaaatgccc ggaagtaatc acctcagtat gcgcaacgat gcaaatacga
     6241 agttgaaact tattcattta tatgatggcg tgcatggtag tttttataaa acaaacccgc
     6301 gttgatctat gaaaatatgc tattgtttca tatcagcagt tcttcttctt tgcagttgca
     6361 aggaagagga actgcttacc tgggatccat acgaatatcc tgaagatccg gaaccaccaa
     6421 atgaacttat ctggcaagtg cctttacatc aagatacatt gggatgtctt gccacgtggc
     6481 ctcaaattta tcagaatgca attctttgca gtggtgaaga aggctgggaa aatccggtgc
     6541 gcgtttttgc cgtagatact tccggaaatc tgatgtggga aagcaatgcc gttttcaatg
     6601 aaaattgctc aagcatttct gcaagcgtcg gcaccggagc ttatcaatat caaaatatgg
     6661 tagccatgct ctgcaatgca gatccaagag tggtaaatat tcaaacaggt gaagtgatgt
     6721 ggcattatga agttcccgga tcagggaatc atagtcctta tatgtcggga ttcggcgacc
     6781 agatatttca tgcttatgcc gatggaccta atccttacac gaaaagttat atcgcaagag
     6841 caaatattta tacaggtatt tgggataccg tttttagcat agaaatggta gatggctaca
     6901 gcgcagatct ttatcctccc ggcgcatggg tagacaataa cggtgataca attatctgtt
     6961 ttcaaaatcg ccaatggaga aatcctccct atgatgggaa ggtcgatctt tatgcatata
     7021 accttactgc agattcattg ctttggatgt acagtgatat agatcccgat ggtaatagtt
     7081 ctgtattccc tcctgtgtac gccgatggta aagtgttttt cagaggatat tttcatgtgt
     7141 acggattaga tgcaaaaacc ggaaatttaa tttggacttg ggcttcaccc aatgtggcaa
     7201 atgatctgct atataccaat acattggttg ttgatgataa agtacttgtg aaaacaacgg
     7261 gacctgaatt gtttgcgctc gatccggata atggaagtga gatttggcat acctccgggt
     7321 tcggggaaat tccatctgaa ctcgtagcat tcgacgggat gttatacttt agcaccgaaa
     7381 ttgacgggaa gctgaatgcg gtgaacatac aaaatggttc tcttgaatgg accatgacat
     7441 cccccaacca cggacaacca acttcgtcat gggcatattt ttccaggccc gttaccattg
     7501 atcccctgtt gcacagactg tatttgacag acttttttta tctattgtgc attcaactaa
     7561 aatagtcagt atatgagacc agcaacatgt ttgatcatcg cgatttccct catgcaacac
     7621 agcttcgcac agattgatga cgtcaagccc gtaaatgggc atcagcgcat tgatgtcaac
     7681 cttgatctcg gatatcgaat tgaacgaaca ggaagcaacc tcgccgattt tgtgcataca
     7741 acaggattcg gtgcaggcgg cgattatagt atcgggtccg catcattacg cttggttatg
     7801 tcgccatata ttcatgcaca atcaggtaac gatttcaatg aaatgaaagc atattataaa
     7861 gccaagggtt atacccttaa ttcatttaat ggcggaatgt actattctac cggaatgtta
     7921 cttggtgcaa gatatgtaat tcctacccgc cggccttcag gattaccttc gttttatgcc
     7981 gcattgcaat acggttttca agctgctcat acacctgcat gcgatatcaa ttattcaaca
     8041 atcaatggcg atattatttc tatcaaagat gatcaggcta tcgctctcgg accaacatgg
     8101 cgcctgcagc taggcgcgga tgtgctgcgc tccggagacg cattgtattc aattcaagca
     8161 ggcgttcgtc aggaaagagc agcattgccg gtggatagaa ctaccctcgg cgtgcccgga
     8221 ctcgatacgc ccggttcaac agaaatattt caatggaatg gtatctatgc tgtcatagga
     8281 cttggtgttg cattcaatat ggagtagttg aatgctgaaa taattattgt tataacataa
     8341 atcaaaatac ctcagtcaat atagaacgca tagcgtcaac attgcccatc gtgtaataat
     8401 gtatgcaagg cactcccgct tttactagct cgcgcgattg ttcaatacac caggcaacac
     8461 cggccttttt tacagcttct gctgtagtgc atttttcaat ggtatcaatt aacgcctcag
     8521 gaatatctac gtgaaaggtg cgcggaatac tatgtatttg tgatgctgca gttatggatt
     8581 taattccggg aataattgga acagtaatgc cttcagcgcg acaacggtct acaaatgcaa
     8641 aaaactgctg attgtcgaaa aacatttgtg ttacaatata atctgcaccg gcgtcaattt
     8701 tttctttcag atgcttgagg tctgatttta aattcggact ttcaaaatgt ttctccggat
     8761 atccggcaac accaatattg aaatctgttt tgtaaccttg aatagaatcc tcctccaaat
     8821 atttgccgcg attcatatcc actacctgac gaacaagatc tactgcgtga ctgtgaccgc
     8881 cgggttcagg tataaaatct cgttcattct tcgatgcatc tccacgcaac agtaatacat
     8941 tgtcaatacc taaataactc aggtcgatta atgcatcctc tgtttcttct ttggtaaatc
     9001 ctccgcaaat aatatgtgct acagaatcaa tatgatattt aaactggact gccgcacaaa
     9061 taccaacagt tcctggacgc ttgcgaataa atactttatc aaaacgctca tcaccgcgct
     9121 tcttataaat atactcgctg cgatgatagg tgacattgat aaatgcaggc ttaaactcca
     9181 ccaatggatc cagcaccgaa taaatatttt gtatgctctt acccttcaat ggcggtaaga
     9241 tctcaaacga caacaaggtt ttatcggcac gattgaggtg ttcagtaact ttcatcaaaa
     9301 aataaaatga gaagcaaaga taattgatga ggaatagagg aggaaggaaa aaggaggaag
     9361 gaaaaaggag agaggaaaaa ggagagagga aaaaggagag aggtggaacg taagagttag
     9421 caagtttgca agttagcagg tgggtttcga gtcgagctcc cctccgaacc acactcacca
     9481 ctcacccact cacccactat ctttgcatca tgaatattct tcttcttggt tccggaggtc
     9541 gtgaacatgc tatggcttgg aaaatcgctc agtcgaaaca atgcgatcaa ttatttatcg
     9601 cccccggaaa ccccggcact gcatcttgcg gaaaaaatat tgctgtgaag gccacagact
     9661 tccctgctct ggctactgtt tgcctcgaaa acaaaattga tatggtggtt gtgggacctg
     9721 aagatccact ggttgccgga gttgttgatt attttgcatc aaatcccgca attgcgcata
     9781 tacctgtaat cggtccgaat aaacaagctg cccaattgga aggaagcaaa gccttcgcca
     9841 aggcattcat gatacgtaat aaggtgcaga cgccgaccta taaaactttt accgcttctg
     9901 atagtgctgc ggcgcatgaa tatttaaagg gacatcctat gccggttgtt ctgaaagctg
     9961 acggattggc tgccggaaaa ggtgttgtta tttgtcaaac cgcagaagag gcaatcgttg
    10021 aatttgatca aatggtgagc ggaaaattcg gtgtggcagg aaacactgta gtggttgaac
    10081 aatttttaac ggggatagaa cttacggtta tcatattaac tgatggtaaa acatatacca
    10141 tcttaccacc atcaaaagac tataaacgtg taggtgatgg cgataccggg ctgaatacag
    10201 gtggtatggg cgctgtttca ccacctccat tcgctacaga agctttcctc gataaagtga
    10261 aagagaaaat cattgaccct actatgatgg gtttgaaaaa tgaaggtatt cattacaccg
    10321 gatttctcta cttcggcctt ttcaatagca atggcgaacc ttacgtaatc gaatataatt
    10381 gccgcatggg tgatcccgaa acacaagtaa tcatgccgcg catagcgaat gatatcatag
    10441 acatgtataa ggcagtggct caaggcactc ttgcatcaca accactgatc acagatcctc
    10501 gtccatgcac tacggtaatc cttgcctctc acggatatcc cggtgtgttt gaaaaaggtt
    10561 atgccatcac aggtatcgaa aatactgaac aatgtatggt ctttcaagcg gggactgcgc
    10621 tgaataataa tggtgaatta gtaaccgccg gcggaagggt gatggcagtg tcggcttatg
    10681 gcgacgatat cgcatcagca ctgcgaacca gctatatcaa tgcaggcagg atctactacg
    10741 aatctcgtta cttcagacgc gatattggct gggatatgta actatttgca tagtagtttt
    10801 tccgaacaat ttctatacgt gtattgagct ggaacctttt gtttataagg gtttgtgtct
    10861 tttcatgcca ttgactttcc gattttttta tagtggaatc gacgattttc tgtcgccgga
    10921 tttacgcaac tattcgggct tcaaccccgt ttcatcccac ataacagaac aataaactca
    10981 aataacaacg tatgaaaaaa ttgatgacaa tgggcttggt tgcagggata gttgccacgc
    11041 tcgcctcatg ttccgatgat agtttaaatg gcatcaccac tgccgaagct gaaagcaagt
    11101 cgcccggatc atggaaggtt tcctattatt tcgataatag cgatggcgtt agccatgaac
    11161 ttgacgccta taccttcgag atcgctacag atggctcact taccgtttca ggcggcggcg
    11221 caacctatac cggcacttgg gtggtaaaga acagcgatga tgatccgaac tatgacactg
    11281 aaatcgattt cagcattatg ggaaacagtg acatggataa aatcgatggc agttggctca
    11341 tgggaaccat caacgacacc aaaatggagc tcctcgatga ttcagggtct gaagaattgc
    11401 atctttcaag gcaataattc attaatcgtc cacaaaaatg agcaatctta aaaaatttca
    11461 ccaatggcaa atatgtgaca aggtaaattg catgttaata tccgtaaata gggctgaaaa
    11521 gtttattgat tggatattat agataagtaa attactgaat tatgtgacat tattgaaaat
    11581 taatagatgc aactgatgtg ggatttattt cattttattg taaaactaat ctgactgaat
    11641 atgagaacct caagctttct aattgccggc gttgcaactg caatgatatt gctaagtgca
    11701 tgcacaaaga cagacacatt tattctttcc gtttctgatg cggaaaatat aattccgggt
    11761 tcttacaaag tggccacctt catagatatt gcaggtgaag gaacggtata cgaaaattat
    11821 atctttcaat ttacgagcaa tggcgatctg accgctacag atggtactga tacttatacc
    11881 ggcacttggt ctattgagaa tgaaaatgat ccggtgtatg ataaaaaagt tactatttca
    11941 attggtggaa atacggaaat ggatgtgctt aatcatggct ggttggtaga agaagttacc
    12001 gatgcaacct tgtatctcac tgatgataca ggcgtggaat cggttcattt tgtgaaaaac
    12061 tgatccggat ttacaacgta tcaatataag gtgccggctt tcttgacagg aaagccggtt
    12121 ttttttgccc caaacccaag cgtaaaagcg cttacaccca caggcagagc tttttttcgg
    12181 cacaaaccga gacattttat aataatttaa ccaaacaaac gtttggttaa atgaaatctc
    12241 catatatctt tgtcctatgc cggttaaaaa aattgatagg ggcgagctca taaaacaagc
    12301 gctaaacttg ttccgtactc agggttatca caaaacaacc atgtcggaaa tcggaactgc
    12361 ctgtggcctg ttgaagggga gtatctatca ctatttctca tccaaggagg atctcatgcg
    12421 cgaagtgctg gaggtgctcc gcaattatta tcgcgacaaa gtatttattc atgcatttaa
    12481 tgatgccctt accgccgaac aacgattgaa tatgctcgga gaaaaaagca ttgaagtatt
    12541 tgtcaatggc gatggcgctt gcctcatggc aaatattgca atggaaacaa atgatgtagt
    12601 tcctgaattc cgtgctccga ttcgcgcttt cttcgacgac tggacaaatg cacttacgca
    12661 tatctattca gataaataca gcaaggaagc tgcaaccata tttgccggtg aaacaatttg
    12721 tgctattgaa ggcgcagcca tgttgatgcg catatataac gaccgcactt acctggacaa
    12781 agcacatcaa atgattacgg agaaattcaa agtaggcagt aaagcagcat tgctgaagta
    12841 atataaaata aacaacatta aagcatttga taagattaat aaaaatgctc ctcgaagaat
    12901 aaatgttatg aacagaatta tcaaaaaaat tgcagtcctc ggttccggcg ttatgggaag
    12961 tcggattgcc tgtcattttg caaatattgg cattgaagtc ctcctgctcg atatcgtgcc
    13021 attacaagca acggaagagc aattgaaaca acctgccttc cgaaataaaa ttgtaaatga
    13081 cagtctgcaa gccgcaatta aacaaaatcc atctccggtt tattcacaaa aagtgctgaa
    13141 gagaattacc acgggaaact ttgatgatga tatggagcgc attgccgact gcgactgggt
    13201 gatggaagtg gtagtggaaa atctgacaat caagcagcag ctgtttgaaa aggtggataa
    13261 acatcgcaaa caaggaacac tggtaacgtc taacacttca ggtattccaa tcgcattaat
    13321 gagtgaaggc agatccgaag attttcgtcg ccatttttgt ggaacacatt tttttaatcc
    13381 gccgcgctat ctccgtttgc tggaaataat tcccggcccg gatacagaaa aaagtgtgat
    13441 tgatttcctc atgaattatg gtgatcgtta tcttgggaaa accacagtgt tgtgtaaaga
    13501 cactcccgca tttatcgcaa atcgcgttgg tgtatttggc atgatggctg ttcttaaagt
    13561 aatggaagaa atgcaattct ctattgctca ggtagaagca cttacaggac caatttacgg
    13621 ccgacctaaa tctgccacct tccgcactgc cgatgtggtg ggcatagata ccttcgtgaa
    13681 agtggcacaa aatacttacg ccgcatgtcc cgatgatgaa agccgcgata tcttccaaat
    13741 tccggcgtac atcaataccc ttgttgaaaa caaatggctg ggcgataaat ccggacaagg
    13801 attttttaag aaagtgaaaa atgcgcaagg acaaagtgaa attctaactc tcgatctaaa
    13861 tacgcttgaa tatcagccct ccacaaaagc aaaatttgca gcgctggatg cggcgaaacc
    13921 tatcgataat ctaaaagatc gtattcgcac attacatgca acaggcgata aggcatcaga
    13981 attttataat aaactgaatg cactcgtttt taaatatgtt tcacatcgca tccctgaaat
    14041 agctgacgaa ctatataaga ttgatgatgc catgcgtgca ggttttggat gggaactggg
    14101 accatttgaa atatgggatg tcctcggcgt ggaaaaattc gtcgcatgga tggagcaaca
    14161 cggaactact cctgcttctt gggtgaagga aatgttgcaa aagggatata aaagtttcta
    14221 tctggttgaa aacggtaaac gcaaatatta tgacgctcgt tccggaggat atgtgcttat
    14281 tccgggaatg gatgcattca tcatccttga taattatcgt gcacaaaaac cggtatggca
    14341 aaacagtgga gctactttac atgatatcgg cgatggcatt ttgaatctcg aattccatac
    14401 taaaatgaat gtagttggaa gtgagatttt ggaaggcatt caaaaatcaa tcgacattgc
    14461 agaaaagaat tatcgcggtc tggtaattgg aaatgatgca gcaaattttt cggcaggagc
    14521 aaatattgct atgatgttaa tgcttgccat tgaacaggaa tttgatgagc tcgatatggc
    14581 cattcgcgtt tttcaaaata cagttgcacg cattcgcttt tccggaattc cggtagccgt
    14641 tgctcctcac ggattaaccc tgggtggtgg ctgcgaaatg actatgcatg ccgatgtagc
    14701 cgtggccgct gcagaaacat atatcggact tgttgaagtt ggcgccggat taattcctgc
    14761 aggtggaggt accaaggaat tggcattgcg cgcaagtgaa aaatatttca aaggtgatat
    14821 tgaaattcct acactacagg aaatgagtct gaatatcgca atggcaaagg ttgcaactag
    14881 tgcacgtgaa gcctttgagt ttggactctt gcgcaatggt attgataaag aagtaataaa
    14941 tggtgcgcga ttaattgcag aagcaaaaaa acaagtgctg tctctggctg atgctggtta
    15001 tgtgcaacct aaacccaaat cagatattcg agtattgggt cgctctgcac tcggaagttt
    15061 ttatgtcgga atatcatcca tgcgtatgtc gggatacatt tctgaatacg atgaaaaaat
    15121 tgctaagaaa gttgcctacg taattagcgg tggagacctc agcatgccta ccgaagtaaa
    15181 tgaacaatat ttactcgacc ttgaaagaga agcattcctt tctttgcttg gcgaaagaaa
    15241 aacattagag cgcatgcaac atatattaaa aaccggaaag ccattgcgca attaattcaa
    15301 tcaataaaaa gaaaattatg acagaagcat atatagttgc aggactccgt tcaccggttg
    15361 gaaaagcgcc ccgtggcatg ttccgattta tgcgcccgga tgatattgcc gttcaggtaa
    15421 ttacgcagtt gctggcacaa attccacaat tggaaaaatc gcgcatcgaa gattgtatag
    15481 tcggcaacgc tgtgcccgaa gcagaacagg gattgcaaat tggccgacaa attgccatgc
    15541 gcgcattgga taaatcaact gccggtatta ccatcaacag atattgtgca agtggggtag
    15601 aagcaattgc tatcgccaca gcaaaaattc gaagcggcat ggccgattgt attatcgccg
    15661 gcggcactga aagtatgagt ttggtgccta ctgccggttg gaagacttct ttaaattatg
    15721 aagttgctgc tgaacatccc gaatatctta tcggcatggg acttactgcc gaaaatgttg
    15781 caaaagaatt taatatttct cgtgaagatg cagatgccta cgcatttcac tcgcatagaa
    15841 aagcaacaga agctattgac aaaggtttct tcaaaaccgg tatagcacca atagccgtca
    15901 aagaaatata tgtaaatgaa aaaaataagc gcgcagaacg aaatattact gttgatactg
    15961 atgaaggtcc gcgtcgagat accactgtgg aagcgctggc gaaactaaaa cccgtatttg
    16021 cagctaaagg cgtagtaact gcaggtaatt cttcacaaac ttccgatggt gctgcttttg
    16081 taatagtgat gagtgaaaaa atggttaaag aattaaatct tcaacctatt gcccgcatgg
    16141 ttagttgtgc ctctgttggt gttgaaccgc atatcatggg gataggtccg gttgcggcaa
    16201 ttccaaaagc attgaaacaa gcgggcatgt cgctgagtgc gattgattta atagaattaa
    16261 atgaagcatt cgcctgtcag gcactggctg ttattcgcga agcaggatta aatcccgaca
    16321 tcgtaaatat taacggcggc gcggtagccc ttggtcaccc cctcggctgc accggtacca
    16381 aattaacagt tcaaatcatc cacgacctca aacgcctcaa caaaaaatac ggcatgatca
    16441 cagcctgcgt cggcggcgga caagggattg caggggtggt ggaggtgctg taattggtag
    16501 tgagtagtgg gtatcaggta tcaggtatca agtatcaggt atcaggt
//
LOCUS       DAOQOU010000054        16432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1762_length_16432_cov_5.466752, whole genome shotgun sequence.
ACCESSION   DAOQOU010000054 DAOQOU010000000
VERSION     DAOQOU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16432
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1762_length_16432_cov_5.466752"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1932)
                     /locus_tag="PLM90_06540"
     CDS             complement(22..1932)
                     /locus_tag="PLM90_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HMZ89064.1"
                     /translation="MRETSFIDQNKKKWQTFEDNYEREQDPEKVSNLFIQITDDLSYA
                     RTYYPNRSVKIYLNNLAQRVFQNIYKNKVRRRQKFASFWKDELPQKMFEARWQLLFTF
                     ALFMLAFFIGMFSSMHDSNFARFILGDTYVNMTEENIRSGDPMKVYKEMNQVDMFLAI
                     TVNNLQVAFITMIFGIFFGLGTVYFILYNGVMIGVFQYFFIERGLFQESFLTIWVHGA
                     LEVSAIVIAGTAGITLGKGLLFPGTLTRLQSFRVHGMRAVQIFMGIAPIIVLAGINES
                     FLTRYTETPDLIRALLIAVEFGFMIYYFVIYPIRKSRAGFDVKRKSDEIPPNREFSIS
                     LSQVKNNGQVFSDSFLVFRQFMGPIIRTVSLVGILYVAVLFLFVVKFEDVAHNIQIIK
                     AMAIFEAIKDIKSLLMYGNIGSTAENNNGMVLSGGLVLFIANTLSLTIITGMALFIVR
                     SVRDKIFTYSRTAFFKFMLRNGWIILICSIALHCIMLLNLTISRFIFILAVPGIMLII
                     GQIAQRDGAGFAVFSKSGYFFRNIVQVMLLYVPLLLLSIMIVCLSHSLLCFFNVELLA
                     WNVPFSPIIYEAIQDISITLFLIITLFMMIGMIAINMILMYFSINEIESADALQQRVL
                     TLKTNKKEFSKA"
     gene            2059..2784
                     /locus_tag="PLM90_06545"
     CDS             2059..2784
                     /locus_tag="PLM90_06545"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ89065.1"
                     /translation="MRTIDILTTQNVTINYELADVKDRIFASILDVIFQSIGLVVLSV
                     VLASPGIMGEQIFLYFITLPVIFFYHLGFEIFMDGRTLGKKLLGIRVVKLTGTVPSIN
                     DYLMRWALRPIDIFFSLGSIAIMLVSSSEKGQRLGDIVANTTVIKTNSTQRIGLADIL
                     NIRTLENYTPTYPGARQFSEEEMLLLKEVMDKSIKYKNTAHDDALVEACYLVKERLGL
                     EKLPSDKHLFVRTLIKDYVAMSR"
     gene            2872..4680
                     /gene="typA"
                     /locus_tag="PLM90_06550"
     CDS             2872..4680
                     /gene="typA"
                     /locus_tag="PLM90_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMZ89066.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKIMHHCQLFSKEHNELILDNNE
                     LERERGITIVSKNVSVRYKDVKINIIDTPGHADFGGEVERVLNMADGALVLVDAFEGP
                     MPQTRYVLSKAIALGLKPCVVINKVDKENCKPDEVHEAMFDLMFNLGATEQQLDFPTV
                     YGSAKFGWMSNDWKVQTDNVAALLDMVVEHVPAPVYNEGSLQMMVTSIDYSSYIGRIA
                     IGRVVRGSVKPGQPVTLVKRDGTQVKSRIKDLFVFEGLGKAKAEEVQSGDIAAITGVE
                     GFEIGDTIGDFDNPEALPGIPIDEPTMSMLFRSNDSPFFGREGKFVTSRHLRDRLYRE
                     IEKNLALRIEETNSADSFMVFGRGILHLGILVETMRREGYELQLGQPKVILKHIDGQR
                     CEPIETLMVETTEESAGKVIEAVSQRKGEMVMMSTRGDRTHLEFNIPSRGIIGLRTNI
                     LNLTQGEAIMSHRFKAYEPWKGDIPSRINGVLIVHETGTSIAYALNKLQERGYFFIGP
                     GEEVYQGQIIGENSRDNDMVVNATRTKKLTNMRASGSDDKVNLPPPHRMPLEEAMEYI
                     DEDELVEVTPLNIRLRKIHLLEHERKKAGTMVEVDD"
     gene            4763..5755
                     /locus_tag="PLM90_06555"
     CDS             4763..5755
                     /locus_tag="PLM90_06555"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HMZ89067.1"
                     /translation="MKLKDKFVRRLSSKEDKGDEFGFTNRYAEGVQRIIRQDGTFNVK
                     RIGERKLIFHELLNMNWMRFTLFILFYYTVVNLLFATLYLLIDYNGIGMTSDYQVHNN
                     FLVALFFSAQTLTTVGYGSLYPLSGTVSLLAASEALIGLMGFAIFTGLMYGRFANTPH
                     SLRFSKQALIGPYKNGMGIMFRAANARNHNLTELEVKVTLAMVITADGKTSRKFYPLT
                     IDNNRITYFPLNWTMVHHINEDSPLYGMTLQDMSDAEVELLIMIKGFNETSSQEMHAR
                     FSYTHREMVWNAKFKLPYYFDEKGATVFELNKIDDFELLEPVIVTEAVAAAVSE"
     gene            complement(5757..6305)
                     /locus_tag="PLM90_06560"
     CDS             complement(5757..6305)
                     /locus_tag="PLM90_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89068.1"
                     /translation="MQPKYFPSSQIRLILIVCGFAALLAGRATGSMNTLNAVLIIIQL
                     VTLELILRESTIFNTIYYRLLYICSSVIIIGAMMIILHWPYANYVVIMGMSSVAIVYT
                     LRSINKQHKALLDYAKWIWLVLNTIAGILNLLHGQYALMSSYLATGAFFLMMLVFFIS
                     PRTSKGMEQRPPEEDDLPIDQI"
     gene            complement(6315..6785)
                     /locus_tag="PLM90_06565"
     CDS             complement(6315..6785)
                     /locus_tag="PLM90_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMZ89069.1"
                     /translation="MKNKLKEVASIFEQETVSEFKQRIEKLTPVTQHLWGTMTVSQML
                     AHIQQPIMVALGDTKPKQNMFAKLLGGVLRRKYVNREPFTKNLPTASSFVVSDERNFE
                     HEKQDAIQYIERLSALDRSTISGRKHPIFGNLSAEEWDMLLVKHLEHHLRQFGV"
     gene            complement(6810..7343)
                     /locus_tag="PLM90_06570"
     CDS             complement(6810..7343)
                     /locus_tag="PLM90_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89070.1"
                     /translation="MRINTILISAAIALAMISCNKDETPTPDGLMSNGSFENGGSFST
                     DGWSVSNGASDSDVPDGGGSFSLRLNPASLPGEGYADYIIEDLEGSHTFNASCFMRSF
                     DDWPGSITLKKRTADNVVTILATAASSDAGWIEKTISASATFNSGDVLIIHLSAGSTE
                     IPIDSKYVLFDLVTIEE"
     gene            complement(7427..8620)
                     /locus_tag="PLM90_06575"
     CDS             complement(7427..8620)
                     /locus_tag="PLM90_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89071.1"
                     /translation="MIPEIRSAFNAAFTEEKYAAFTLACQERFNKKIEFKICETPVFV
                     SAEFSRSVKAAGEAIIDQLTDQAFLRHTDAAIPDMWRVPGEEGKPVFLALDFGICVDD
                     AGNLIPQLIEMQGFASLYAWQHELGRQYRQFYPIPEEVHHLFNGLDDIAYRKLLRDTI
                     IGDHDPREVVILEIQPDHQKTWIDFYLTREMLGIQPICISDVIVEGTQLFYILNGEKT
                     CIKRIYNRLIFDELAQRNDLSIQWNMTTPADVEWVCHPNWFFRISKYTMPFLKHASVP
                     QTLFLDQVKEIPADLQNWVLKPLFSFAGHGVIFNVTEADIEHAMPQAGNFILQRKVQY
                     AQVVQTPDEPAKAELRLMYLWLPGTDRPTLVTNLARLSKGVMIGVDYNRNKTWVGGTV
                     AYYAD"
     gene            complement(8660..9484)
                     /locus_tag="PLM90_06580"
     CDS             complement(8660..9484)
                     /locus_tag="PLM90_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HMZ89072.1"
                     /translation="MSRERKIKLAILDMYDNAPNEGMRCLKAMVQAVGDKIDWEVFEV
                     RNKDEVPDKNFDIYISSGGPGSPFDGEGKTWEPKFFNLVDQITAHNANGHSRKKYMFA
                     ICHSFQLLSRHFELGEVCKRKGTAFGVFPIHKTEHSKRDRYFRNLPDPYYVVDSRDWQ
                     LIEPDFAKMTALGASIMAMEKKREHVPLPRAVMAIRLSDEMYMTQFHPEADAEGMLRL
                     FAKPEKRETIRNSHGDWKLEEMVRFLSDQDKIPLTHKEVIPNFLRSSIGALTMSDT"
     gene            9642..13145
                     /locus_tag="PLM90_06585"
     CDS             9642..13145
                     /locus_tag="PLM90_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ89073.1"
                     /translation="MKQTTLGAKLVVLLNLCVLSVTRSTAQAPAKPNIVFIMTDDARY
                     DEFRPSGGADWFIAPNIERIANEGINFNRTYAPTPICGPSRASIYTGVYSHQSGTQNN
                     GDDLSETLPLIQNILIDDGYYTGFIGKYGNGFPSPNQFDYWVDIGDQELYKNLWIKVN
                     GTNVFVSGHLTDAFNTYLNAFMDSAAVHNDKPFALFFFPLAPHTPNVPRATDAAMYNA
                     EVKPFPENFYPYDTLYPTYYTDPGSLWVKDTTATVKFIQDRYSCLYGVDENVSKIMLH
                     LDDTHETDSSIIFFTSDNGYLIGEHMMRAKVMPLEESIRVPLYVRYPAWFPAGQFIDN
                     DLAELIDIPKTILDAIGIPDTFGFMGKSLRDLAEPDTLRPYAFYEYEGTEPDDVHHVP
                     DIRGVRSFDYLYTTTNCDCWTEEFYDLTTDPLQDTNQVANPKYHEKILEYRAILAQLR
                     LEKFDTLPFESGDCGLYGSYEIPDDIDNDCDGIIDNDLDAYIRYQDIDNDGYGDPATS
                     MIVFGELPGYVNNPNDCNDTSASIHPAAIEICDGIDNDCDGFIDDADPDVVGMTTWYF
                     DNDFDGLGNDTVQIVSCFAPIGYISVGGDCNDNNPAYTIGGPEICNGIDDDCDGLFDD
                     ADPDITGQTIWYADNDGDGLGNDTITIIACGAPEGYVITFGDCNDSNADFTIGSPEIC
                     NAYDDDCDGLVDDADPDITGQTIWYADIDNDGYGDWANTSLNCFAPEHYVAIFGDCND
                     ADSLQTHGTPEVCNLLDDDCDGLTDDEDPDVTGKILFYADMDSDGYGNITMTTLNCFL
                     PIGYVTNGLDCNDLHSGIHPLASEICDGIDNNCNLLVDDADPLITGRPTWYLDADFDT
                     YGSALISVEACNQPVGYVDNNSDCNDLNGLVHPGVADYCDLINNDCDAFLDEDPVNPV
                     ITASGPVEFCKGSSVTFTASPIISGFNRQWYKGTSPIAGATGITYTATQSGSYKVKYT
                     APAGCITYSAITTVTVNNNPKPVVSLTSASNDLCVNNPVKLSCKNKIGSTSQWYKGAV
                     PLFGEVTNKYNATTSGNYKVKQIDPTGCFGTSSAYSVVQTCHEASPYQDEADASFVIY
                     PNPSQGDFTLQIDFGADINGEASVKIYSMLGQMMYEAFIIVDNGEIDDQLNVSGQIPS
                     GVYLIEATIDQQKLVREIVIE"
     gene            complement(13224..14330)
                     /locus_tag="PLM90_06590"
     CDS             complement(13224..14330)
                     /locus_tag="PLM90_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HMZ89074.1"
                     /translation="MSIYKDFTLGIEEEFQIIDPETRDLSHRKHHIIDAAAKYNVDIK
                     AEMHQSVVEAATTICKDIHDARNQVVKLRRIIADLADKDNLKIAAAGTHPFAAWEEQL
                     ITNHPRYQEIVNEFQEAARSNLIFGLHVHIGMPSRDVAVFMMNSLRYFIPHIFALSTN
                     SPFWEGRDTGFKSFRTKVFDKFPRTGIPDVFNDASHFDNFIALLVKTNCIDNGKKIWW
                     DLRVHPYFNTIEFRMCDIPMRADETIALAALMQALTVKLYKLMKSNIGWRNYDRALIQ
                     ENKWRAARYGISGKLIDFGKQEESETRGLVLELLDFVDDVVDELGSREALENVRWILD
                     NGTGADRQVKMYYEKNRDFKALADYMIDETYIGI"
     gene            complement(14348..15343)
                     /locus_tag="PLM90_06595"
     CDS             complement(14348..15343)
                     /locus_tag="PLM90_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89075.1"
                     /translation="MKRIGIVHGMEDSFPNAFIERINSKRIDGIVAERVLIDKVVQGA
                     ASGYDVILDRISHDAPFYRAYMKNAAISGAAVMNNPFWWSADEKFFNNALAVKIGIPV
                     PNTVLLPSHDLPENTKDTSFRNLKYPLDWESIFNYIGFPAYMKPHDGGGWRGVSRLEN
                     MEEFFSKYAETGDDVMILQSEVTFDYYFRCYCMGGKYVHIMYYEPRNPHHLRYTWNGP
                     QAPQSLLDTVKTYTLALNQALGYDFNTVEWAVKDGIPYAIDFCNPAPDADLQSVGEEN
                     FEWVVEHAALMAIERALAHKPGQDNLNWGTYVRNSVGGVPHLPQTQPIFNSALSY"
     gene            complement(15365..16156)
                     /locus_tag="PLM90_06600"
     CDS             complement(15365..16156)
                     /locus_tag="PLM90_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HMZ89076.1"
                     /translation="MFRIRVRLFSTTFACKTAITLELNEQKHTWQSSVLGIQMEMLSF
                     GTGGFPLIIFPTSMGMHNENRDFKLIESIGWFIETGMVKVYCPDSIDKHSWYNKEASP
                     ADRVLNHMLYDRMIMEEIIPGITKDSGYSRIGVAGCSFGGYHACNFAFRHPDLTAVMI
                     SLHAKFDITGQLDGYYDDNVYYNNPPDFVPGLSDEQLWKMQIFLGSAEYDMCLDANYH
                     MAHLLGTKQVPHVLEVEQGDKHDWPCWRKQLPRFLSLIQYDNLHS"
     gene            16199..16417
                     /locus_tag="PLM90_06605"
     CDS             16199..16417
                     /locus_tag="PLM90_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89077.1"
                     /translation="MKLTLTPQVTVHRTAIRSSFLNREVQLTVILPPDYRQKPYPVLW
                     LNDGQDIDPMGVADILQQAMVSNKDIIS"
BASE COUNT         4762 a         3492 c         3356 g         4822 t
ORIGIN      
        1 gcaataatat caacaaccac ctcatgcctt actgaattct tttttgtttg ttttcagggt
       61 taatacgcgt tgttgtaagg cgtcagccga ttctatttcg tttatgctga aatacattaa
      121 tatcatgttg atggcaatca tacctatcat cataaacaat gtgataatta ggaatagcgt
      181 aatgctgata tcttgtatcg cttcatatat gatcggacta aatggcacat tccatgcaag
      241 tagttctaca ttgaaaaagc ataggagaga gtgcgacaaa catactatca tgatgctcaa
      301 taataacagt ggaacgtaca gcaacatcac ctgaacgata ttgcggaaga aatatcctga
      361 tttactgaaa acggcaaacc ccgctccatc acgctgcgcg atttgaccga tgataagcat
      421 tatgcccggt actgcaagta tgaaaatgaa tcttgaaatg gtaagattca aaagcattat
      481 gcaatgcaat gcgatgctgc aaattaaaat aatccatcca ttgcgaagca tgaatttaaa
      541 aaaagctgtc cgactgtaag taaatatctt atcgcgcaca cttcgaacga taaacaaggc
      601 catgccggta atgatggtaa gtgataaagt attggcaata aataacacca ggccgccact
      661 caataccata ccattattat tttcggccgt gctgccgatg ttgccgtaca ttaacaatga
      721 cttaatatcc tttatcgctt caaatattgc catagcctta atgatctgaa tattgtgggc
      781 gacatcttca aatttgacaa caaataaaaa caaaacggca acatacagta tgcctacaag
      841 tgaaaccgtt cgtataatag gccccataaa ttgacggaat accaaaaatg aatcggaaaa
      901 tacctgacca ttatttttca cctgtgaaag tgaaattgaa aattcgcgat tgggcggtat
      961 ctcgtcagat ttgcgtttta catcaaatcc tgctcttgat tttctaatcg ggtaaatcac
     1021 gaaataataa atcatgaacc caaattcgac agctattaat aaagctctga ttaaatccgg
     1081 cgtttctgta tagcgcgtca gaaaactttc attgatgcct gccaacacaa tgattggtgc
     1141 aatacccata aatatctgca cagcgcgcat tccgtgtact ctaaaagatt gcaatcgcgt
     1201 taaagtgcca gggaaaagca accctttgcc tagggtaatg cctgctgttc ctgcaattac
     1261 tatggcagag acctccaatg ctccgtgcac ccaaatggtt aaaaatgatt cctgaaaaag
     1321 tccgcgttca ataaagaaat attgaaatac gcctatcatt acaccattgt acagaataaa
     1381 ataaacggtg cccagcccga agaatatgcc aaatatcata gtgataaatg ctacttgcag
     1441 attattaaca gtaattgcaa gaaacatatc tacctgattc atttctttgt aaaccttcat
     1501 cgggtctcca gaacgaatgt tttcttcggt catattgaca taggtgtcgc ctaaaataaa
     1561 tctggcgaaa ttgctgtcgt gcatactgct gaacatcccg ataaaaaatg ccagcataaa
     1621 taatgcaaaa gtgaacagca gttgccacct ggcttcaaac attttttgcg gtaattcatc
     1681 tttccagaag gaagcgaatt tctgcctgcg acgaacttta ttcttgtaaa tattttgaaa
     1741 aacgcgctgg gcgagattgt tcaggtagat ttttaccgaa cgattcggat agtaggttcg
     1801 ggcataggaa aggtcatctg ttatctgaat aaacagattg cttaccttct ccggatcctg
     1861 ctcacgttcg taattgtctt caaacgtttg ccacttcttc ttgttctgat ctataaaact
     1921 ggtctcgcgc atgttactga ggatgcagtg gtaaatataa gagatttgct cgtagattgt
     1981 gaaattcata cctattgctc accatttacc taacaatgtg agcggatttt aaaaaaaatg
     2041 tatttttgtt ttgattacat gcgcaccatc gatatcctta ctacccaaaa tgtaaccatc
     2101 aactacgaac tggctgatgt gaaagaccgc atcttcgcga gcattttgga tgttattttt
     2161 cagtcgatag gtttggtggt actctctgta gttttggcct cgccgggcat catgggcgaa
     2221 caaattttcc tttattttat aacacttcct gttatattct tttaccacct tggctttgaa
     2281 atcttcatgg acggtcgtac ccttggtaaa aaactccttg gtattcgagt ggtaaaactt
     2341 accggaactg ttccctccat caacgattat ctcatgcgct gggctttgcg tcctattgat
     2401 attttctttt ctctgggctc cattgctatt atgctggtat catcctccga gaaaggtcag
     2461 cgattgggtg atattgtggc taataccact gttattaaaa cgaattctac ccagcgcatt
     2521 ggcctggctg atatcctgaa catcagaact cttgaaaatt atacccccac gtatcccggc
     2581 gccagacagt tttctgagga agaaatgctg cttttgaaag aggtgatgga taaaagcatc
     2641 aaatacaaga acaccgccca cgacgatgcc ctcgtagaag cctgttatct ggtgaaagag
     2701 cgcctgggac tggaaaaatt gccttcagat aagcacctct tcgttcgcac cctcatcaag
     2761 gattatgtgg ctatgagtcg ctaatcatgt taataatatc tgaagtgcgg cacttattca
     2821 ccgatttcct gcgttattgc ctgtaaaaac gctacctttg cagccgtttt tatgcagaag
     2881 atacgcaata tagccatcat cgcccacgtc gaccatggta aaactacgct ggtagacaag
     2941 atcatgcacc attgtcagct tttttcaaaa gagcacaatg aactcattct tgataataat
     3001 gaactcgaac gcgaacgtgg tatcaccatc gtgtctaaaa acgtttctgt tcgttataaa
     3061 gatgtgaaaa tcaacattat agatacaccg ggacacgccg atttcggagg tgaagtggaa
     3121 cgcgttctca atatggccga cggtgctctc gtgctcgttg atgcctttga aggtccgatg
     3181 ccgcagaccc gctatgtgct cagcaaagcc atcgcgctgg gtttgaagcc ttgtgtggtg
     3241 atcaataaag tggataagga aaactgtaag cccgatgaag tgcatgaggc tatgttcgat
     3301 ttgatgttca acctcggtgc aactgaacaa cagcttgatt tcccgactgt ttacgggtcg
     3361 gccaagttcg gatggatgag caacgactgg aaagtgcaaa ccgacaacgt tgctgcgctc
     3421 ctggatatgg ttgttgagca tgttcctgct cctgtttaca acgagggttc tctgcaaatg
     3481 atggttacca gtatcgacta ctctagttat atcggtcgta ttgccatcgg tagagtggtg
     3541 aggggttcgg taaaacccgg acaaccggtt acgctcgtaa aacgcgacgg cacgcaggtg
     3601 aaaagtcgta tcaaagatct ttttgtattc gaaggacttg gaaaagcgaa ggctgaagag
     3661 gtgcaaagtg gcgatatcgc ggctattacc ggtgttgaag gcttcgaaat tggcgatact
     3721 attggcgatt tcgataatcc tgaagcctta cccggcattc ctatcgatga gcctaccatg
     3781 agcatgctgt ttagaagtaa tgactcacca ttcttcggtc gcgaaggtaa atttgtcacc
     3841 agccgccacc tccgcgatcg cctgtatcgc gaaatcgaga aaaatcttgc ccttcgcata
     3901 gaagaaacca atagtgccga ttctttcatg gtattcggtc gcggtatcct acacctcgga
     3961 atcctggtgg aaaccatgcg ccgtgaaggt tatgaactgc agctcggaca accgaaagtt
     4021 atcctcaaac atatcgacgg acaacgttgt gaaccaatcg agaccttaat ggtggaaacc
     4081 actgaggaat cggcaggtaa agttatcgaa gccgttagcc agcgaaaagg tgaaatggtc
     4141 atgatgtcaa ctcgtggcga ccgcacccat cttgagttca atatcccatc aagaggtata
     4201 atcggtttgc gtaccaatat cctgaattta acacaaggtg aggctatcat gagccatcgc
     4261 tttaaagcct atgagccgtg gaaaggtgat atcccttcgc gtatcaatgg tgtgttgatc
     4321 gtgcatgaaa caggcacctc cattgcctat gcattgaata aactgcaaga acgtggatat
     4381 ttcttcatcg gccccggtga ggaagtatac caaggtcaga tcatcggtga gaacagcagg
     4441 gataacgata tggttgtaaa tgccactcgt accaaaaaac tgaccaacat gcgcgcaagc
     4501 ggaagcgatg ataaggtgaa tctgccacca cctcaccgca tgcctctgga agaagccatg
     4561 gaatatatcg acgaagatga actggtggaa gtaacgccgc tcaatatccg tctccgtaag
     4621 attcatttgc tggagcatga gcgtaagaaa gccggcacca tggttgaggt tgatgattaa
     4681 gattgtcagt cgcacatttt aggaactatt tcttcttgta tttttgttgt ccttcctcta
     4741 cagggatagg gtcaatatca ccatgaagtt aaaagacaaa tttgttcgtc gccttagcag
     4801 caaggaagat aagggtgatg aattcggatt caccaatcgc tatgccgaag gcgtgcaacg
     4861 tatcattcgt caggatggca catttaatgt aaaacgaata ggggagagaa agctcatatt
     4921 tcacgaattg ctgaatatga attggatgcg gttcacgctc tttatattgt tttattatac
     4981 tgtagttaac ctgttgtttg caaccttata tctgcttatc gattacaatg gaatcggcat
     5041 gaccagcgat tatcaggtgc ataataattt tcttgttgcc ttgtttttca gcgcacaaac
     5101 attaacaacc gttggttatg gatcattgta tccattatcc ggtacggtca gcttactcgc
     5161 tgcatctgaa gcattaatag gattaatggg attcgccatt tttacgggat tgatgtacgg
     5221 taggtttgcc aatacaccac atagtttaag attttcgaaa caggcattaa ttgggccata
     5281 taaaaatgga atgggaataa tgtttcgcgc agcaaatgcc agaaatcaca atctcactga
     5341 attggaagta aaggttacat tggcaatggt aattactgcg gatggcaaaa caagtagaaa
     5401 attttatccg cttaccatcg acaataatcg cattacttat ttcccgctca actggaccat
     5461 ggtgcatcat attaatgaag acagtccatt atatggcatg accttgcagg atatgtcaga
     5521 tgcagaagtg gagttgttga ttatgatcaa aggattcaac gaaacttcat cacaggaaat
     5581 gcacgcaaga ttctcctata cacatcgtga gatggtgtgg aatgcgaaat ttaaattgcc
     5641 ttattatttc gatgagaaag gagctaccgt atttgaactc aataaaattg atgattttga
     5701 attgttggaa cctgtaattg ttactgaagc agttgcagcg gctgtttcag aataatttaa
     5761 atttgatcaa tcggcagatc atcttcttcc ggaggccgct gttccatgcc ttttgaagtt
     5821 cttggggaaa taaaaaatac cagcatcatt aagaaaaatg caccggttgc caaataggat
     5881 gacatgagtg catactgccc gtgcaacagg ttcaaaatac ctgctatggt attcaataca
     5941 agccatatcc attttgcata atctagtaat gccttgtgtt gtttgttgat actgcgcaaa
     6001 gtatatacaa tggcaacaga tgacatgccc atgataacta cataattggc ataaggccaa
     6061 tgtaaaataa tcatcattgc tccgatgata attactgagc tgcagatata tagcaatcga
     6121 taataaatgg tattgaaaat tgtgctctca cgtaaaatga gttccagtgt taccaattga
     6181 ataatgatta aaacagcatt caaggtattc atgctgccgg ttgccctgcc tgccagcaaa
     6241 gctgcaaatc cgcaaacaat gaggattaat ctgatttgtg atgaagggaa atatttcggc
     6301 tgcatgatat tacattacac accaaattgg cgcaagtgat gttcgagatg tttcacaagt
     6361 agcatgtccc attcttcggc acttaaattt ccaaatatcg gatgcttgcg accggaaatg
     6421 gttgatctgt ctaatgctga taaacgttca atatattgaa tagcatcttg tttctcgtgt
     6481 tcaaaatttc tttcatcaga tacaacgaaa cttgaagcag tgggtaagtt cttggtaaat
     6541 ggttctctat ttacatattt tctcctgaga acaccaccta ataatttcgc aaacatgttt
     6601 tgcttaggct tggtgtcacc caaagctacc attatgggtt gttgaatatg tgccaacatc
     6661 tgcgatactg tcatcgtgcc ccacaaatgt tgtgttacgg gtgttaattt ttcaatgcgt
     6721 tgtttaaatt ccgatacggt ctcttgctca aaaatacttg ccacttcctt taatttattc
     6781 ttcaaaaaaa tatttccttt tttatctaac tattcctcaa tcgttaccaa atcaaataac
     6841 acatatttac tgtcaatcgg tatttcagta gatcctgcag ataaatgaat gatcaacaca
     6901 tcgccggaat taaaagttgc acttgccgaa attgtttttt ctatccatcc ggcatcactg
     6961 cttgcagctg ttgctagaat ggtgacaaca ttatctgcgg tacgtttttt taatgtaatg
     7021 gatcccggcc aatcatcgaa tgaacgcata aaacatgagg cattgaaagt atgcgaccct
     7081 tcaaggtctt caatgatata atctgcatat ccttctccgg gtaaggaagc aggatttagt
     7141 cttaaagaaa agctaccgcc gccatcagga acatcgctgt ctgaagcacc gtttgatact
     7201 gaccatccat cggtagaaaa tgagccacca ttctcaaagg agccattcga catcaatcca
     7261 tcaggtgtag gcgtttcatc cttattacag gaaatcattg ctaaggctat agctgcgctt
     7321 atcaggatag tgttaattcg catatccatt tttttctaaa gatatacttc caaacggtaa
     7381 atggattgga acaagtgtaa gaatgctgta attaatttac cgcaactcaa tcggcatagt
     7441 aagcaaccgt gccgccaacc catgtcttat ttctattata atctacacca atcataaccc
     7501 ctttactcaa ccttgcaaga ttcgtaacga gtgtaggacg atcggttccc ggtaaccata
     7561 aatacatcaa tcgcaattca gctttagcgg gttcatcggg ggtctgaacc acctgtgcat
     7621 attgcacttt gcgttggagg ataaaattac ctgcctgagg catcgcatgt tcgatatcgg
     7681 cttctgtaac gttgaaaatt accccatggc cggcaaaaga aaacagtggt tttaataccc
     7741 agttttgcag atcggcagga atttctttca cctgatcaag aaataaagtt tgcggaacag
     7801 aagcatgctt taaaaatggc atcgtgtact tgctgatgcg gaaaaaccag tttgggtgac
     7861 aaacccattc cacatctgca ggagtggtca tattccattg gatagaaaga tcatttcgct
     7921 gcgcaagttc atcaaaaatg agcctgttgt aaattcgctt aatgcaggtt ttctcgccgt
     7981 ttagtatgta gaaaagttgt gtgccttcaa caatgacatc agaaatgcaa attggctgta
     8041 ttcccaacat ttcgcgtgtc agataaaaat cgatccaggt tttctgatga tcgggctgaa
     8101 tctcaagtat caccacctcc cgcgggtcgt gatctccaat tatggtgtct cttagcaatt
     8161 ttcgataggc gatatcatct aatccattaa aaagatgatg cacttcctcc ggaatcgggt
     8221 aaaattgcct gtattgtctt cctaattcat gttgccaggc ataaagcgag gcaaatccct
     8281 gcatttcaat gagttgtgga atcagattgc cggcatcatc tacacaaatt ccgaaatcaa
     8341 gggctaaaaa tacaggtttc ccttcctcac ccggaacccg ccacatatca ggtattgcag
     8401 catctgtatg tcgcaaaaag gcttgatctg tcaactgatc aatgatcgct tctcctgcag
     8461 ccttgaccga tcttgaaaac tcagcactca caaataccgg tgtttcgcag attttaaact
     8521 caattttttt attaaatcgc tcctgacagg ccaatgtaaa tgcggcatac ttttcttcgg
     8581 tgaatgcggc attaaaagca cttcgaattt ccggaatcat ggatgggaaa aaattaataa
     8641 gtaattgata atcaaataat tatgtgtccg acatggttag cgcaccgatg gaagatcgca
     8701 agaaattagg gataacctct ttatgcgtca gcggaatctt atcctggtct gaaagaaagc
     8761 gtaccatctc ctccaatttc caatctccgt ggctattacg gatggtttct cttttttcag
     8821 gtttggcgaa caaccggagc ataccttctg catcagcttc agggtgaaat tgggtcatat
     8881 acatctcgtc gctcagcctg attgccatca ccgcacgggg taggggaaca tgctctcgct
     8941 tcttttccat tgccataata ctggctccaa gcgcggtcat tttggcaaaa tcgggttcta
     9001 tgagctgcca atcgcgacta tctaccacat aatatgggtc gggcaggttt ctgaaatacc
     9061 tgtcccgctt cgaatgttcg gttttatgaa tagggaatac cccaaaagcg gtgccttttc
     9121 gcttacacac ttcgccaagt tcaaaatgcc ggctcagcaa ttgaaaagag tgacatatgg
     9181 cgaacatata cttctttctg ctatgtccat tggcattatg tgctgttatt tggtcaacaa
     9241 gattgaagaa ttttggctcc catgtcttgc cttctccatc aaatggactg cccggtccgc
     9301 ctgaggagat ataaatatca aagttcttat ccggaacttc gtctttattc ctcacctcaa
     9361 atacttccca gtctatttta tcgccaaccg cttgtaccat ggctttcaga caacgcatgc
     9421 cctcatttgg ggcattgtcg tacatatcta atatcgccag tttaatcttc cgctcgcggg
     9481 acataaggtc caaaattaga gtttttaaaa agtgcagata tgtttgttat ccaccactta
     9541 tcatcaaaat gtcaatatgc gtcaatttgt ttcttatagg gcagatttaa tggctatatt
     9601 tgctgcatcc cattattgaa ccaaacagtc aacacatacc aatgaaacaa acgactcttg
     9661 gggctaagct ggtcgtgctt cttaaccttt gtgtcttatc ggtaactcgt tcaactgctc
     9721 aagctccggc taaacccaat attgtcttca tcatgacaga tgatgcacga tatgatgaat
     9781 ttcgtccaag tggcggagcc gactggttta ttgcaccaaa tattgaacgc attgcgaatg
     9841 aaggcatcaa cttcaatcgg acgtatgctc caactcccat ttgcggaccg agcagggcat
     9901 ccatttacac cggtgtgtat tctcatcaga gtggcaccca aaacaatggc gatgatcttt
     9961 cagaaacgct cccattaatt cagaatattc tgatcgatga tggttattac accggattca
    10021 tcggaaagta tggaaatggt tttccgtcgc caaaccaatt tgattattgg gtggatatcg
    10081 gcgatcagga gttatataaa aatctatgga ttaaggtaaa tggtaccaat gtatttgtat
    10141 caggacacct tactgacgca tttaatacct acctcaatgc gtttatggac tcggccgctg
    10201 tacataatga taaaccattt gcattatttt tctttccgct tgctccacat acaccgaatg
    10261 ttccgcgagc aactgacgcg gcgatgtaca atgctgaggt gaagcctttt cctgaaaatt
    10321 tttatcctta cgatacttta tatcccacgt attatactga cccgggttca ctttgggtaa
    10381 aggatacaac agcaacagtc aaatttattc aggatcgata cagttgtctt tatggtgttg
    10441 atgaaaacgt gtctaaaatc atgttacatc tagacgacac gcatgaaaca gacagctcta
    10501 ttattttctt tacgagcgac aatggctatt taattggcga acatatgatg cgcgctaaag
    10561 tgatgcctct tgaagaatct attcgtgtgc cattgtatgt gcgctatcct gcatggtttc
    10621 cggcaggtca atttattgat aatgaccttg ctgaattaat cgatatccca aaaacaattc
    10681 tcgatgcaat tggtattccc gacacctttg gatttatggg gaaatcattg cgcgaccttg
    10741 ctgaaccgga tacactcaga ccttatgctt tttatgaata tgaaggaaca gaacccgacg
    10801 atgtacatca cgttcccgat atcagaggtg tgagaagttt tgattatttg tatacaacta
    10861 ctaattgcga ttgctggaca gaagaatttt atgatctgac cactgatcct ttgcaggata
    10921 ccaatcaggt ggctaatccg aaatatcacg aaaaaatttt agaataccgt gccatacttg
    10981 ctcagttgcg tttagagaaa ttcgatacac tgccttttga atccggagat tgtggtttgt
    11041 atggaagtta tgaaataccg gatgacattg ataatgactg cgatggtata attgataacg
    11101 acctggatgc ctatatcaga tatcaagata ttgataacga tggctatggc gatccggcaa
    11161 ctagtatgat cgtttttggt gaattacccg gctacgtcaa taacccgaat gattgtaatg
    11221 atacatcagc ttctattcac ccggctgcaa tagagatttg cgatggcata gataatgatt
    11281 gtgacggatt tatcgatgat gctgatcctg atgttgttgg aatgacaaca tggtatttcg
    11341 ataatgattt tgacggtctt ggaaatgata cagtccaaat agtaagttgc tttgccccga
    11401 ttggatatat cagtgttggt ggcgattgca atgataataa tcctgcttac actatcggag
    11461 gtccggaaat ttgtaatgga attgatgatg actgcgacgg gttgtttgat gacgctgacc
    11521 ctgatattac cggtcagaca atttggtatg ctgataacga cggcgacggc ctcggaaatg
    11581 atacgatcac cattatcgct tgcggtgcac cggaaggtta tgtaattact tttggtgatt
    11641 gcaatgatag caacgctgat tttactattg gttctcctga aatttgcaat gcgtatgatg
    11701 atgattgcga tggattagtc gatgacgcag atcccgacat cactggtcaa actatctggt
    11761 atgcagatat agataatgac ggttatggcg attgggcaaa taccagtctc aattgctttg
    11821 ctcccgaaca ctatgttgcc atttttggcg attgtaatga tgccgattcc ctgcaaacac
    11881 acggcactcc ggaagtatgc aatttattag atgatgattg cgatggactt accgatgatg
    11941 aagaccctga tgtaacaggt aaaattttgt tctatgccga tatggacagc gatggatatg
    12001 gaaacatcac tatgaccact ttaaattgtt ttttgcctat cggatatgtt acaaatggtc
    12061 ttgattgcaa tgaccttcat agtggtattc atcctcttgc ttcagaaatt tgtgacggaa
    12121 ttgacaacaa ctgtaatttg cttgttgatg atgctgatcc actcataaca ggtcgcccta
    12181 cttggtatct cgatgcagat tttgatacct atggttcggc tttgatcagt gtagaagctt
    12241 gtaatcaacc ggtaggttat gtagacaata atagtgattg taatgacctt aacggtctcg
    12301 tgcatcccgg tgttgcagat tattgcgatt taatcaataa cgactgcgat gcctttcttg
    12361 atgaagatcc ggtaaatcct gtaattactg ccagtggtcc tgttgaattc tgtaagggaa
    12421 gttctgttac ttttacggct agtccgatta ttagtggatt taatcgccaa tggtataaag
    12481 gtacatctcc aattgccggt gcaacaggta ttacttacac tgctacccaa tccggcagtt
    12541 ataaagtgaa atatacagct cctgcaggtt gtatcaccta ttcagcaatt accactgtca
    12601 ccgtaaataa taatcctaag cctgttgtgt cgctcacatc ggcatcaaat gatctttgtg
    12661 tgaataatcc ggtgaaattg agttgcaaga ataaaattgg gtctacttcc cagtggtata
    12721 aaggggcagt gccgttgttt ggagaagtga ccaataaata taatgccact acctccggaa
    12781 attacaaagt gaaacaaatt gatcctacag gatgcttcgg tacatcttca gcatattcgg
    12841 ttgtgcaaac ctgccacgaa gcaagtcctt atcaggatga agctgatgca tcattcgtga
    12901 tttatccaaa tccaagtcag ggagatttta cgcttcaaat tgattttggt gcagatatca
    12961 atggcgaagc ctccgtgaaa atttattcaa tgctcggtca aatgatgtac gaggcattta
    13021 taattgtaga taatggtgaa atagacgacc aattaaatgt ttccggacaa attccttcgg
    13081 gagtatattt gattgaagca actattgacc aacagaaatt agtgcgcgaa attgtaatcg
    13141 agtaaaaaaa atattgcaac aaaaaaagcc atcggtttcc cggtggcttt tttattatgc
    13201 ttgattaacc tgtttacatt acatcatata ccaatatatg tttcatctat catataatcg
    13261 gcaagcgctt taaagtcgcg gttcttttca taatacatct tcacctgacg atcagcaccg
    13321 gtgccattat ctaatatcca acgcacattt tccaatgctt cgcgactgcc caactcatct
    13381 actacatcat ccacaaaatc gagcagttct aatactaagc cgcgcgtttc tgattcttcc
    13441 tgtttaccga aatcaatcag tttaccactg atgccgtaac gagcggcacg ccatttgttt
    13501 tcctgaatca atgcacgatc gtaatttctc caaccgatat tgctcttcat caacttatag
    13561 agcttaacgg taagtgcctg cattaaagct gctaaggcaa ttgtttcatc ggcgcgcatc
    13621 ggaatatcgc acatcctgaa ttcgatggta ttaaagtatg gatgcacacg aagatcccac
    13681 catatttttt tgccgttgtc gatacaattt gtttttacca acaatgcaat aaaattatcg
    13741 aagtggctgg catcattaaa cacatcagga ataccggtgc gcggaaattt atcaaatact
    13801 ttcgtgcgga atgatttaaa tccggtatct cttccttccc aaaatggcga gtttgtagac
    13861 aatgcaaaga tgtgtggaat gaagtatcgc aacgaattca tcataaacac cgccacatca
    13921 cgcgatggca tgccaatatg cacatgcaat ccaaaaatca gattactgcg cgctgcttcc
    13981 tgaaattcat tcacgatctc ctgataacgc ggatggttcg ttatcaactg ttcttcccat
    14041 gctgcaaatg gatgtgttcc cgctgccgct attttcagat tgtccttgtc ggcaagatct
    14101 gcaatgatcc tgcgcaactt taccacctga ttgcgggcat cgtgaatgtc tttacagatg
    14161 gtagttgcag cttcaactac actctggtgc atctctgctt ttatatcgac attatatttc
    14221 gcggccgcat caataatatg atgtttgcgg tgcgatagat cgcgtgtttc gggatcgata
    14281 atctgaaact cttcctcaat gcctaatgtg aaatctttat aaatactcat gtgcagtcgt
    14341 caacaaatta ataggataat gccgaattaa atatcggttg tgtttgtggt aaatgcggca
    14401 ctccgccaac agaattgcga acataggttc cccaattcag attgtcctgg cccggtttat
    14461 gtgccaatgc gcgttctatt gccatcagcg cagcatgttc aacaacccat tcgaaatttt
    14521 cttcacctac actctgtaaa tcagcatcag gtgcaggatt gcaaaaatct atggcatatg
    14581 gaataccatc tttaacagcc cattctaccg tattgaaatc ataacccagt gcttgattca
    14641 atgcaagcgt ataggttttc acggtatcca acaaactttg tggtgcctga gggccattcc
    14701 aggtataacg gaggtgatgc ggatttcgtg gttcgtaata cataatgtgt acgtatttac
    14761 cgcccatgca ataacaacgg aagtaataat caaaagtaac ctcactttgc aaaatcatta
    14821 catcatcgcc ggtttcagca tatttactga aaaactcttc catattttct aaacgcgaaa
    14881 cacctctcca tccaccaccg tcatgtggtt tcatatatgc aggaaatccg atgtaattaa
    14941 atatcgattc ccagtccaac ggatatttca aattccggaa ggatgtatct tttgtgtttt
    15001 caggtaaatc atgcgaaggc aataacaccg tgttaggaac aggaattccg attttaactg
    15061 ctaatgcatt attaaaaaat ttctcatcag cactccacca aaaaggatta ttcataacag
    15121 cagcaccact gatggcagca tttttcatgt aggcacgata aaatggcgca tcgtgtgaaa
    15181 tgcgatcaag aatcacatca taaccgctgg ctgcaccttg cactaccttg tcgatcaaca
    15241 cacgttcagc aacaatgcca tctatgcgtt tactgtttat tctttctata aaggcattag
    15301 ggaatgaatc ttccatgcca tgtacaattc ctattcgttt cattgcaaat tatttagggt
    15361 taattcaaga gtgtaaattg tcgtactgta tcaaagataa aaatctaggc aattgttttc
    15421 gccagcaagg ccaatcatgt ttatcgcctt gctccacttc aagcacatgt ggtacctgct
    15481 ttgtaccaag taaatgtgcc atatggtaat tagcatctaa acacatgtca tattcggcag
    15541 agcctaaaaa tatctgcatc ttccacaact gctcatcgct caatcccgga acgaaatccg
    15601 gcgggttgtt ataatacaca ttgtcatcgt aatacccgtc aagctgtccg gtgatatcga
    15661 atttagcatg caatgaaatc atcacagccg ttaagtcagg gtgacggaaa gcaaaattgc
    15721 aggcatgata accaccgaaa ctacatccgg caacaccaat gcggctataa cctgagtctt
    15781 ttgttatccc gggaatgatt tcttccatga tcattcggtc gtaaagcatg tgattcaata
    15841 ccctgtcggc cggagaagct tccttgttat accagctgtg tttatctatg ctgtcgggac
    15901 aatacacttt taccattccg gtttctataa accatcctat gctttctatc agtttaaaat
    15961 cgcgattttc attgtgcatg cccatcgatg tagggaatat gatcaaagga aatccgccgg
    16021 ttccaaaact caacatttcc atctgtatac caagcaccga agattgccag gtatgctttt
    16081 gttcattcag ttccaaagtg attgcagttt tgcacgcaaa ggtagttgaa aacaagcgaa
    16141 cacgaatgcg aaacaagtgc acagagttgt gttgtttaca ttaaatttgc gtctatacat
    16201 gaagttgaca cttactcctc aggttacggt tcatagaact gctatcaggt cgtcgttcct
    16261 gaacagggaa gtgcagctca cagtgattct acctcccgat taccgccaaa agccctatcc
    16321 tgtgttgtgg ctcaacgacg gacaggatat agaccctatg ggtgttgcag atatcctgca
    16381 gcaggcaatg gtttctaata aggacataat aagttaaaag ttctcatggc ag
//
LOCUS       DAOQOU010000055        16190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1814_length_16190_cov_5.556306, whole genome shotgun sequence.
ACCESSION   DAOQOU010000055 DAOQOU010000000
VERSION     DAOQOU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16190
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_16190_cov_5.556306"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLM90_06610"
     CDS             <1..448
                     /locus_tag="PLM90_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89078.1"
                     /translation="RKVIDNTGFAGRWQWLQDHPTLIADCAHNSGGLAALFSQVNTLT
                     YENLHIVTGSVNDKDLDASLSLYPTSAIYYFAKPDIPRGLEAETLQNKAFGFGLKGSV
                     FDSVRDSLYAALNKAGKNDLVLICGSIFVVAEALPAYRDREKALSK"
     gene            460..1890
                     /locus_tag="PLM90_06615"
     CDS             460..1890
                     /locus_tag="PLM90_06615"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HMZ89079.1"
                     /translation="MIRRFYILLTFTFLATALTAQSDTGHEIHVRIDGIRDTTIMLGH
                     HYGSKQYVIDTVRIDHNGDVVFAGNDTLPGGIYLVVVPQLKNRYFEMVISGQDQHFSM
                     HTDTLDFVKHMDVKGSTENSLYYQDVNFIAEKKDEMNAMKTKLDALPKDDPEAEKIKE
                     AMKELNKTVEDTRSAIIKNNPGLFYAKFLQAVTDVHIPEAPMNPDGTKDSTFALHYIQ
                     KHFFDNIDFNDERLLRTNLFDTRIHKYFEDYIYKVPDSIEKGVDFVIGKAAVNKKIFQ
                     YVTVMLLNDYATSKMMGFDAVYVYIVDKYYSTGKAYWLDDVGLYRIQQQAERVRPTLI
                     GKTGQPLILQDINGHDIPLYAVDRRFTVILFWSPTCGHCKKELPKVEKIYPELKELGA
                     EIYAVYNEEEFDKWEDWLHQHNYPWINVCDRNNKEMVGVKYNVDMTPLIYVLDHNKKI
                     IGKKISIEQVPDLLKNQIAIENAHKP"
     gene            2084..2491
                     /gene="msrB"
                     /locus_tag="PLM90_06620"
     CDS             2084..2491
                     /gene="msrB"
                     /locus_tag="PLM90_06620"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMZ89080.1"
                     /translation="MSKKQYAIIKSDQEWQDMLTPEQYQILRKKGTERPFTGEYDHFF
                     GSGVYRCAGCGQLLFDSTSKFDSGCGWPAFDKEIADGNIDYESDNSFGMRRVEIMCSN
                     CGGHLGHVFDDGPTETGQRFCVNSASLKFDEKK"
     gene            complement(2494..3540)
                     /locus_tag="PLM90_06625"
     CDS             complement(2494..3540)
                     /locus_tag="PLM90_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ89081.1"
                     /translation="MKNASPGRIVLLAALTVFSGILLLEALPLFIPALDKVAYQFIAV
                     PILVFILTYRVLYLAVEKFIYRKIKLIYKNILANKSTKALTEERLQMDADVIGQVQDE
                     VNSWAEEKRSEIAILHEQANFRKEFLGNVSHELKTPIMSIQGYLENLQDGGIEDPGVN
                     YLYVEKALKNVERMAAMIDDLVDISKLESGELNIKKSKFDICSLTKDVFEALEIQSEQ
                     SRISLSIKEGCDHIYLVNADQKLIYEVLINLISNAIKYGKKEGFVHVGFYRMGDRILT
                     EISDNGVGIAQEHLTRVFERFYRIEKSRSRDNGGTGLGLSIVKHIIEAHDQIVNVRST
                     EGEGSTFSFTLDVA"
     gene            complement(3555..4238)
                     /locus_tag="PLM90_06630"
     CDS             complement(3555..4238)
                     /locus_tag="PLM90_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ89082.1"
                     /translation="MANEKIRILIVDDEPDILEVIEYNLLKEDYQVIKAFNGREALEK
                     ARVEKPNLILLDIMMPEMDGIEVCRELRSNHAFDNTLIAFLTARNEDFTQVQGFDVGA
                     DDYITKPIKPRLLVSRIKALLRRTGSADQNHEQVSYGDISIDEEKYLVYVKGKPISLA
                     KKEFELIQLLVSRPGKVFTRQEIFNKIWGMDVIVGDRTIDVHIRKIREKVGEDYIKTI
                     KGIGYKFDQ"
     gene            4381..6975
                     /locus_tag="PLM90_06635"
     CDS             4381..6975
                     /locus_tag="PLM90_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ89083.1"
                     /translation="MAEQTPNTGLAWKLVLAFTFSMALIGFAAWYAYDSFNKLLGSVE
                     TLSAPDNSDSMLQGLVNSMHAADNDMRNYIMSNDNTSLQSYISTMEHISVELDNLKQD
                     IAFEGQETSLDTLEEYFQDKADLMISLVIFKQSGEYERLNSKAYNKLSTKANESPDIS
                     TIQQNVAIKDPTIKQPAIKQPEVTDATSKKKDKKNKDEGFLRNLFSGKNDKKSDVDAD
                     PTIVALKDTSSNTSNTSNVTIDLAQTSTVDVEDVRRILQEINKEQRRYNTQLSVKELE
                     ILASDKLIIEKIDEVIQRMKIRQQTGIAGQLAIAKDNANASSRTMLIIAAVALLAGLV
                     FMTVILMDIIRSNRYKIQLIEARNRAEYLAKAKEEFLANMSHEIRTPLNAIIGFSEQI
                     EHTPLQPEQKKFIKAVSNAGTHLLNTVNDILDLSKIEAGKLNIDDHTFSIREVIEEVV
                     SILEVKAHEKGLALTTAIGEHADEMVVGDPFRIKQILYNIAGNAIKFTEQGFVEISCS
                     AKKGRKGIVYEIAVKDTGIGIAPDKLLRVFEIFEQAESSITRKYGGTGLGLSISKKLA
                     NIMDGDILVNSKPGEGSEFTLHIPLRLASADEIASVEKDKTEYADLSGLEILLVDDEP
                     YNLMLAEVILNKYGAHITTAANGREALDLIDIKSFDIVLADLHMPEVDGYMLANKIRE
                     RYLQVPLIALTANVMQNDVERIRTSGFNDILLKPYKEVALLQIISKYAPVVFGKQLLE
                     VPEIRESIVEKATMDLFDLSEVMQFADNDPDVLSSIVEAFITNNKQNVELLRDAVHDY
                     NLDNVRNIAHKMLPSYNHFHVYSIVPELKEMEVISPAANGSLVEKFDKVQHVSNLLFA
                     NLRSAITR"
     gene            complement(7013..8452)
                     /locus_tag="PLM90_06640"
     CDS             complement(7013..8452)
                     /locus_tag="PLM90_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ89084.1"
                     /translation="MRPTVLIIDDDPTFCLMLKSFLTKKDFATETAFSGAEALNQVRK
                     QDFDVVLSDFRLPDTDGLELLENIREIKPGTPVIIMTSYADIRIAVKAMKIGAYEYVT
                     KPVNPDEILLIVRNALNREKEKEAMPAAIPASKPQQQSGNKKNPPKNGFEYVEGNSVV
                     AKRIMEYIDVVAPTNLSVIIHGESGTGKEYVAKMIHQKSNRSHKPFVSIDCGALSKEL
                     AASELFGHRKGSFTGAIVDKTGQFVFAEGGTLFLDEIGNLTYEIQVQLLRAIQERRIR
                     KIGSNQDVEVDVRIIVATNEDLQDAVKKGNFREDLYHRLNEFKIIVPSLRDREDDINI
                     FAEHFLQLANAELNKDVVNFDDEVKAIFRRYSWPGNLRELRNVVRRAVLLTSGEYVRK
                     ETLPHEIVFEKREDVSNNQSVRTENKMQADQPLDLKSLAEKTERELIINTLIKVNYNK
                     SKAAALLKIDRKTLYNKMKLYKILTDDES"
     gene            8613..13922
                     /locus_tag="PLM90_06645"
     CDS             8613..13922
                     /locus_tag="PLM90_06645"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ89085.1"
                     /translation="MNLTLRNLLKVFVLSLGMLSVGNLKAQYCIPDYTIGTGDGDYID
                     GVVLGDISNFSGMGDEYNDYTDLSTLLNPGSTYDLEVYNTPSWPENYAAWIDWNQDEV
                     FSDDERLNPIDLELTAGGSDVITFTVPFTALPGTTRMRVMCVYFPALAIDPCGVVETY
                     TFGEVEDYGINIPAAGPYDLGVTSISGLGTGCGLGVTPITVTVTNLGTEPSGAFSVAY
                     QVDDPTLGALAPVVQAYTGAAIPSLGSVAFTFTTPADFSNLGEYTLQAYTIYGVDMVA
                     ADDTSDETFTNIPIVTTFPYYQDYEDGAGGWISGGTESTWELGDPAGFAIIGPPPATP
                     TSLNSWTTELTDYYNLNEKSYVISPCLDFSSLIIPYMEFDANWDIQLYDDGAKLQYTT
                     DEGLTWNDLGGIGTGDNWYNTNTCYAMWPTFYIDSYNGWSGNSGGWVHSKHDLSFLAG
                     EPSVKFRFVFASSSYWNFNDGFAFDNMWIGDPLPNDIGVTNVISPETSPFLSATESVT
                     VEVTNFGTNPQTGFPVSYQVDGGTIHTETFTGTLGVAEVATFTFAATSDLSADGDYDF
                     VAWTGLASDEDVTNDTWSETISNIEPITGTNAYYIYSNSTGFEPWYTTSNSQHMDDVF
                     GVGEWTTEYYETIDPAVLFSLDNCFIFMEGSDSHADAMETFINANMDAMQLWVAAGGH
                     LLINAAPNVGDGMDFGFDGVHLDYPWYASTVSASDPTHPVWDGPFVPTSTDMTGFNYA
                     HASISGAYLSPILFDAATPSRIICAERNYGAGTVIFGSMTVTDFHTPLTEAGNFRKNL
                     IAYLATCTISNDDVGVYSIESPETGCGMTDEEIVTIKVKNYGFLPQTDVPVYYQADGG
                     TIVSEVVPGTIGVGDVVLYTFATPVDISLIGDHTLNAWTGLPADTINDNDTTYSTLAN
                     IPVITTYPYYENWELGDEDGWMAYGASSTWELGYPDGPVINEPPTATPGSQYSWLTSI
                     TGNYNDNENSYLEGPCFDLSSLVIPYVEVDLWWETEDTWDGMQLEYSTNGGVTWSVLG
                     DIGTGDNWYTNTAFALDYETGWVGSGGGWVTSHHDISFLAGETDVKFRFKFSADGIIN
                     FDGIGLDNFKVQDPWPNDVGVTDLITPVSGVDLTTTETVSVTVKNFGTLPQSGFPVNF
                     QADAGTIHTETFTGTLTAGASATFTFAATADLSAEGLHEICAWTSLATDDDVTNDEIP
                     GCADVMHLAPVSGTGAYYIYSSTVGFEPGWMNSNSTAMDEVFGDDAWTLDYYEDIDPY
                     TVFSESNCFIFLEASEGHWSEFESFFEDNSDLIQGWVASGGNLFINASPWEGDGGDVG
                     FGGVSIVYPYYTYQTAATDPTHPIYFGPFTPTTSDFTGFYVAYARTTGDVTPIMNDFY
                     CDDCYILSQKDWGDGRVFFGNMSAPEYYSPSPDNQNLRQNIIDYLKLCAPVDLGVTTL
                     LSPDGGCGTTATETVSVIVSNFGPSTVTNIPMKFQVDGGAIVTEVADGPVPIGGTYTY
                     TFTATANLATPGTHTLATWTDFSGDEDETNDTLISSIVSLATPVLDLGPNTTVCDDVV
                     LNALNVGSTYLWSTGETTQTITVTTSGTYSVTVTNPTSGCSITDNITVTVNYTPVANF
                     SYTVTGGTVTFTNESTDGASYNWSFGDGGSSTTANPSHAYATAGMYTVTLTVTNGCGS
                     DFYSVVIEVGTAITDMELDQSVTVYPNPTDGRTNVNIALSEAQNITLELLNQIGQTVW
                     TNNPGNILTESINIDMTNMAAGVYNLKVTGDNGTATKQIILTK"
     gene            14106..>16190
                     /locus_tag="PLM90_06650"
     CDS             14106..>16190
                     /locus_tag="PLM90_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89086.1"
                     /translation="MKKHLTLLLSCMLTLAFMAPTRDAIAGGDNNYYIYSNAYGGYEP
                     WYTTSNSDAMNAVFGVGGWLQDYFETCDPALVFSANTNFVFLEGSDGFADELESFLSV
                     NIDVIETWVEGGGRLLLNAAPNEGDGMSFGFGGVNLTYWGGSSNVHATDPTHPIFNGP
                     YTPTGTSWTGSSFSHASVSGPDLTPLIIDDFTPTTIALSEGRYGTDGIVFFGGMTTDN
                     FHYPSLESINLFQNILNYLAFYEIPDHDIASLSLASPSSGCGMGVETVSVNYKNYGTN
                     DEVNIPVSYQLDGGAVVSATIAGPVAPGETVTYNFAVPADITALGAHTFVISCDLATD
                     EIPANNTITVTINNIPVVDVFPYYQDFEAGDGGWIAGGGASSWELGSPAGFAIVGPPP
                     ATPGSVNSWTTSLTGYYNDGEKSYIESPCFDFSSLVFPYLELDLNWDTQMYSDGAKIQ
                     YSTDAGATWSDLGDVGTGENWYTSYNCYGMWPDFYVTDYRGWDGNSGGWQHGYQDLAF
                     LGGQPSVKLRMVMGADLYWSYYDGFAFDNVKISDLFPNDVGVSNITAPVSGPLLSATE
                     SVSVAVTNYGTLAQSGFPVSYRFEGGPVHTETFTGTVNPGETVNYDFATTEDLSVLGD
                     YTFEAWTSLATDEDMSNDSITEVISNLEPLTGDNAYYIISNTTGGEPWYTVSNSQSMD
                     AVFGVGEWTMGYF"
BASE COUNT         4658 a         3312 c         3390 g         4830 t
ORIGIN      
        1 gaggaaagtg attgataaca caggttttgc aggaagatgg caatggttgc aggaccaccc
       61 cacccttatt gcagattgtg cgcataacag cggtggattg gctgcattgt tcagtcaggt
      121 gaacacgctt acgtatgaaa atttgcacat tgtaaccgga tctgtgaacg acaaagatct
      181 cgatgcttct ttgagcttat atccaacttc ggccatatat tattttgcga aacccgatat
      241 tcccagagga ttagaggctg aaaccttaca aaataaggct tttggcttcg gactgaaggg
      301 ttcggtgttt gattctgtga gggattcatt atatgcagcg ctgaataaag caggcaaaaa
      361 tgaccttgta ttaatatgcg gcagtatttt tgtggtagca gaggcactcc cggcatatag
      421 agatcgggaa aaggcgttat caaagtagcg aaaccaatta tgattcgaag attttacata
      481 ctcctcactt tcaccttttt agcaacggcc ttaacagcac aatctgatac aggtcacgaa
      541 attcatgtca gaatagacgg catcagagac actaccatta tgctgggtca tcactatggc
      601 agcaaacagt atgtgataga caccgtccgc attgatcata atggcgatgt ggtttttgca
      661 ggtaatgata cactaccggg cggaatatat cttgtagtag tgccacagtt gaaaaataga
      721 tattttgaaa tggtgatcag cggacaggat caacatttca gcatgcatac cgatacgctt
      781 gattttgtca agcatatgga tgttaaggga tctaccgaaa actcgttata ctaccaggat
      841 gtcaatttca ttgctgaaaa gaaggatgaa atgaatgcga tgaaaactaa attggatgcc
      901 ttgccaaaag atgatcctga agcagagaaa attaaggagg caatgaagga actaaacaag
      961 actgtagaag atacacgttc agccatcatc aaaaataatc ccggactttt ttatgccaaa
     1021 tttcttcagg ctgttactga tgtgcatatc ccggaggcgc ctatgaaccc cgatggaaca
     1081 aaggattcga cgtttgcatt gcactatatt caaaagcact ttttcgataa tattgatttc
     1141 aacgacgagc gattgttacg taccaattta tttgatacgc gtattcataa atactttgaa
     1201 gactatattt ataaggttcc ggattcaatt gaaaaaggtg ttgattttgt aataggaaaa
     1261 gcagcagtca acaaaaaaat atttcaatat gtaacagtga tgttactcaa cgattatgct
     1321 acatctaaaa tgatgggttt tgatgcagtg tatgtttaca tcgttgataa atattattct
     1381 accggtaaag catattggct cgatgatgtt ggactttatc gtatacaaca gcaagctgaa
     1441 agagtacgcc ccaccttgat aggtaaaacc gggcagccgc tgatcttaca agacataaat
     1501 gggcacgata tacctctgta tgcggttgac aggcgattta ccgtgatttt attttggagc
     1561 ccaacgtgtg gtcactgtaa aaaggaattg ccaaaagtgg aaaaaattta ccctgaatta
     1621 aaagagctgg gtgccgaaat atatgctgtt tataatgagg aagaatttga caaatgggaa
     1681 gattggcttc atcagcataa ttatccgtgg atcaatgtat gcgacagaaa caataaagaa
     1741 atggtgggcg taaaatataa tgtagatatg acgccattga tctatgtatt agatcacaac
     1801 aaaaaaatca ttggtaagaa aatatcgatt gagcaggtgc ctgatttact gaaaaatcag
     1861 attgctattg aaaatgccca taaaccttaa tcaaaaatcg aaatttcgtg gtgcgtcaat
     1921 aatgccatac ctatcatgat tgacatgatt ccgaaaaata gtatgatggc aaacacatat
     1981 ttcgagtatc ccggtttcat agcggttgtt tgactgttaa acaagtaatt gttgagaatt
     2041 gttcatgaac ttttagcagg ttgaatatgt atgtaataat tgtatgtcga aaaagcaata
     2101 cgccatcata aaatcagatc aggaatggca ggatatgctt actcctgagc aatatcagat
     2161 ccttcgcaaa aaggggacgg aacgcccgtt tacaggtgaa tatgaccatt ttttcgggtc
     2221 aggagtttac cgctgtgccg gttgtggtca gttgttattt gattctacat caaagtttga
     2281 cagcggatgc ggttggccgg cattcgataa agaaatagcc gacggcaata tcgattacga
     2341 atctgataat tcttttggca tgcgacgcgt agaaataatg tgtagtaatt gcggcggtca
     2401 ccttggacat gtgtttgacg atgggcctac agaaactggt cagcgttttt gcgtgaattc
     2461 ggcttcgctg aagtttgatg agaagaaata atcctaggca acatctaggg tgaagctgaa
     2521 tgtactgccc tccccttctg tactgcgcac atttactatc tgatcatgtg cttcaataat
     2581 gtgtttaacg attgagagac cgagaccggt tccgccatta tctctgcttc gacttttctc
     2641 aatgcgatag aaacgctcaa atacgcgtgt caaatgttct tgcgctatac ccacgccatt
     2701 gtctgaaatt tcagtaagga ttctatcgcc cattcggtaa aaaccaacat gcacaaaccc
     2761 ctctttttta ccgtatttaa ttgcgttgct tattaaattg attaacactt cgtaaatgag
     2821 cttttgatct gcattcacca ggtatatatg atcacaacct tctttaatgg ataatgaaat
     2881 gcgcgactgt tcagactgta tttccaacgc ttcgaaaaca tctttggtca aggaacagat
     2941 atcgaacttt gattttttaa tgtttaattc acctgattcc aatttgctga tatctacgag
     3001 gtcgtctatc atggcagcca tgcgttcaac attcttcaag gccttttcaa catataaata
     3061 attaacgccc ggatcttcaa tgccgccatc ctgaaggttt tcaaggtatc cttgaatact
     3121 cattatcggt gtctttaact catggcttac gttccctaaa aattctttgc ggaaatttgc
     3181 ttgttcatgc agaatggcaa tttctgaacg cttttcctct gcccatgaat ttacctcatc
     3241 ctgcacctga ccaattacat cagcatccat ttgaagccgt tcttccgtaa gtgctttggt
     3301 agatttattt gcaagtatgt ttttataaat caattttatc tttctgtaaa taaatttttc
     3361 taccgcaagg tacagtacgc gatacgtaag tataaaaacc agaatcggca ctgcaataaa
     3421 ttgataagca actttatcta atgccggaat aaataatggc aaggcctcca acaatagaat
     3481 gccggaaaat acagtcaatg ctgccagtaa aacaatgcgc cccggtgagg catttttcat
     3541 agaaatgaaa tgaattattg gtcaaattta taaccaattc ctttgatggt cttaatgtag
     3601 tcctctccta ccttctcacg gatctttcta atatgtacat caatggtcct gtcccctact
     3661 attacatcca ttccccatat tttgttaaat atctcctgac gggtaaaaac tttccccggg
     3721 cgactaacca gcaattgaat gagttcaaac tccttttttg ccaatgaaat gggctttccc
     3781 ttcacataaa ccagatactt ttcttcatct atactgatat cgccataact cacctgttca
     3841 tgattttgat cagcactccc tgttcgtcgc aataaggctt taatacggct tacaagcagt
     3901 ctgggcttga ttggtttggt gatataatcg tcggctccga catcgaaacc ctgcacctga
     3961 gtgaaatctt cgtttcgtgc cgttaagaag gctattaagg tgttgtcaaa tgcatgattg
     4021 ctccttaatt cacggcaaac ttcaatacca tccatttcgg gcatcatgat gtccaggaga
     4081 atgagattgg gcttctcaac ccgggctttt tccagggctt ctctcccgtt gaaagccttt
     4141 attacctgat aatcctcttt cagcagattg tattcgataa cttccagaat atccggctca
     4201 tcatctacaa tcaaaatcct aatcttctcg ttagccatat gctattttga tgtaaagcta
     4261 tgatgatgat attaatagat gttgaatcca atattaatat aatgttaaag ataccgtttc
     4321 tttacgttga acgggctaaa ggcttcaata tgagcaaaat cgcttaattt taagtgcgca
     4381 atggcagaac aaactcctaa cacaggatta gcatggaaac tggtgctggc cttcactttc
     4441 tcgatggccc tgatcggttt tgctgcctgg tatgcctatg attccttcaa taagctgcta
     4501 ggatcagttg aaacactctc cgcgccggat aattcagatt ccatgctaca gggattggta
     4561 aacagcatgc atgccgctga taacgatatg cgtaactata tcatgagcaa tgataatacc
     4621 agcttgcagt cgtatatcag caccatggag catatcagtg ttgaactgga taacttaaaa
     4681 caagacattg catttgaagg ccaggaaaca tcgctcgata ccttggaaga gtactttcag
     4741 gacaaagcgg acttgatgat ttcattggtt attttcaagc aatcaggtga gtatgaaaga
     4801 cttaatagta aggcctacaa taagctttca acgaaggcaa atgaatctcc tgatatttca
     4861 actatccaac aaaatgtagc aataaaagac cctacaatca agcagccggc aatcaagcaa
     4921 cctgaggtta ctgatgcaac atcaaaaaag aaggacaaga agaataaaga cgaaggattc
     4981 ctccgcaact tattttccgg taaaaacgac aagaaatctg acgttgacgc tgatccaaca
     5041 attgtagcat taaaagatac atcgtccaac accagtaaca cctccaatgt caccattgac
     5101 ctggcacaga cttcgacagt agatgtcgag gatgtgcgca gaattcttca ggaaataaat
     5161 aaggaacaac gcagatataa tactcagcta tcagttaagg agcttgaaat tttggccagc
     5221 gataaactca tcatcgaaaa aattgatgag gtaattcaac gaatgaaaat tcgtcagcaa
     5281 accggtattg ccggtcagtt agcaatagct aaagataatg caaatgcctc ctcacgtacg
     5341 atgttgatca tcgctgctgt tgctctgctt gcagggttag tttttatgac ggtgatattg
     5401 atggatatta tacgcagcaa taggtataaa attcaattaa tagaagccag aaaccgtgcc
     5461 gaatatctgg caaaggctaa ggaggagttt cttgccaaca tgagtcacga aattcgcact
     5521 ccgctaaatg ccattatcgg tttttctgag cagatagaac acactcctct tcaaccggag
     5581 caaaagaaat tcatcaaggc agtatcaaat gccggcacac atttattgaa tacggtaaat
     5641 gatattcttg atttatcaaa aattgaagcc ggtaagttaa atatcgatga tcatactttt
     5701 tctattagag aagtaatcga agaagtagtg tcaattttgg aagtaaaagc gcatgaaaaa
     5761 ggtttagcac tcacaacagc aatcggcgaa catgccgatg aaatggtggt gggagaccct
     5821 ttccgtatta agcaaatctt atataatata gcaggcaacg cgataaagtt tacagaacaa
     5881 ggctttgttg aaattagttg ctctgcaaaa aaaggaagaa agggaattgt ttatgaaatt
     5941 gccgtaaaag ataccggaat tggtattgcg ccggataaat tattgcgcgt attcgagatt
     6001 ttcgaacagg ccgaatcttc catcaccaga aaatatggcg gcacaggatt gggattgtct
     6061 atttcgaaaa aattggcaaa tatcatggat ggcgatattc ttgtcaacag caaacccggt
     6121 gaaggctcag aatttacttt gcatatacca cttcgattag catccgcaga tgaaattgcc
     6181 tctgtagaaa aagacaaaac tgaatatgcc gacctgtcag ggcttgaaat tctgttagtt
     6241 gatgatgagc cttataatct aatgcttgcc gaggttatcc tgaataaata cggcgctcat
     6301 attaccactg cagctaatgg acgcgaggct ttagatctta tcgatattaa aagtttcgat
     6361 atagtgcttg ccgacttgca tatgcctgaa gttgatggtt atatgctggc gaacaaaatt
     6421 cgtgagagat atttgcaagt gccattgatt gcactcactg caaatgtgat gcagaatgat
     6481 gttgaacgca ttcgaaccag tggatttaat gatattttac tcaagccgta taaagaagtg
     6541 gctctgttgc aaattatatc gaaatatgct cccgtggtat tcggaaaaca attgttggaa
     6601 gttcctgaaa tcagggaatc catagttgag aaagccacaa tggatttatt tgatttgagt
     6661 gaagtgatgc aatttgccga caatgatccg gatgtgcttt caagcattgt agaggctttt
     6721 attaccaata acaaacaaaa tgttgaactt ttgcgcgatg ccgtgcatga ttacaatctg
     6781 gataatgtaa gaaatattgc acacaaaatg ctgccttcct acaatcactt ccatgtttac
     6841 agtattgtgc ctgaattgaa agaaatggag gtaatctctc ctgcagcgaa tggaagcctg
     6901 gtagagaaat ttgacaaagt gcagcacgtg agcaatctac tctttgccaa tttgcgttca
     6961 gctatcacaa gatgatctgt aagactgaat gctaaagata ttattgaaat aattacgact
     7021 cgtcgtctgt tagaatttta tacaacttca ttttgttgta taatgttttg cggtcaattt
     7081 tcaataatgc cgccgccttg ctcttattgt aatttacttt tatcaaggtg ttgataatta
     7141 gctcacgttc ggttttttct gccagcgatt ttagatccag aggctgatca gcttgcattt
     7201 tattttcagt gcggacagat tgattatttg aaacatcttc gcgtttttcg aaaacaattt
     7261 catgaggaag tgtctctttt ctgacatact cacctgaagt aaggagtact gctcttcgca
     7321 caacattgcg cagctcacgc aaatttcccg gccagctata gcgcctgaaa atagctttca
     7381 cttcatcatc gaaattaact acatccttat tcaattctgc attcgcaagt tgaaggaagt
     7441 gctcagcgaa aatattaatg tcatcctccc tgtcgcgcaa cgaaggaaca atgattttaa
     7501 attcatttaa tctatgataa agatcttccc tgaaattacc ttttttaacg gcatcctgca
     7561 gatcttcatt ggttgcaact atgatgcgca catctacttc cacatcttga ttactaccta
     7621 tttttcttat ccttcgttcc tgaattgcac gcagcaattg aacctggatt tcataggtga
     7681 gattaccaat ttcatcaaga aacaaagtgc caccttctgc aaacacaaat tgaccggttt
     7741 tatctacaat agcacctgta aatgatcctt ttcgatgtcc aaacaattca ctcgccgcca
     7801 attctttact caaagcacca cagtcaatcg acacaaatgg tttatgcgaa cgattgctct
     7861 tttgatgaat catttttgct acatactctt taccggttcc actttctccg tgaatgatca
     7921 ccgataaatt tgttggagct acaacatcaa tatattccat gatgcgtttg gcaaccacgc
     7981 tatttccttc cacatattca aacccgtttt taggcggatt ctttttgttg ccggattgtt
     8041 gctgtggttt acttgcagga atagctgccg gcatggcttc cttttccttt tcacggttaa
     8101 gtgcgtttct gacgatcaat aaaatttcgt cgggatttac cggttttgtg acatattcat
     8161 atgcgccaat tttcattgcc tttaccgcaa ttcgaatatc tgcataggat gtcatgataa
     8221 taaccggtgt tcccggtttg atttcacgaa tattttcaag caattcgagc ccatcagtat
     8281 ccggaagtcg aaaatcgctt aataccacat caaaatcctg tttgcgtacc tgattcaatg
     8341 cctcagcacc ggaaaatgct gtttcagtag caaaatcctt ctttgtcaaa aagcttttaa
     8401 gcataagaca aaaagtgggg tcgtcgtcga taatcaggac tgttgggcgc attgtggtat
     8461 accgggtggt gtttaaagga cgcctaaaga tagagactct ataccaaacg gatgtaaatt
     8521 ttcggtggac agactgtttg ttaatttttt ttgatttggc gggcaggtac gctagatttg
     8581 agactccttt cttttcatat taaaaagcga ctatgaattt aactttacgc aatttgctta
     8641 aagtattcgt tctttcattg ggtatgttgt ctgttggcaa cttgaaagcg caatactgta
     8701 ttcccgacta cacaattggt acaggcgatg gcgattatat tgatggtgtt gtccttggcg
     8761 atatcagcaa tttctccgga atgggtgatg agtataatga ttatactgat ctctctacac
     8821 tccttaatcc cggatctact tacgatcttg aagtgtataa cactccatca tggcctgaaa
     8881 attatgccgc ctggatcgac tggaaccagg acgaggtgtt cagtgatgat gagcgtttaa
     8941 atcctatcga tttggaatta accgcaggcg gatcggatgt aatcacattc acagtgcctt
     9001 tcaccgctct tccgggaaca acacgtatgc gcgttatgtg tgtgtatttt cctgcccttg
     9061 caattgatcc gtgtggagtt gtagaaacat ataccttcgg cgaagtagag gattatggta
     9121 tcaatatccc ggccgccggc ccgtatgatc ttggcgtcac ctctatctca gggctgggaa
     9181 ccggctgcgg attgggagta accccaatta ctgtaactgt cactaacctt ggcacagaac
     9241 cttccggagc attcagcgtg gcttatcagg tagatgatcc aacactcgga gctttggcac
     9301 cggttgttca ggcctataca ggtgctgcta tcccttcttt ggggtctgtc gcctttacat
     9361 tcaccacacc tgccgatttc agtaatctcg gagagtatac gcttcaagcc tatactattt
     9421 acggtgttga tatggtagca gctgatgata cttcagatga aacgtttacc aatattccaa
     9481 tagtaaccac cttcccttat taccaagact atgaggatgg tgccggcgga tggatttcag
     9541 gtggaacaga aagcacctgg gaattaggcg acccggcagg atttgccatc atcggccccc
     9601 ctcctgctac tcctacaagt ctcaacagtt ggaccacaga actgaccgat tattataacc
     9661 tcaatgaaaa atcgtatgtg atctcaccgt gtttggactt tagttcactc atcatacctt
     9721 atatggaatt tgacgccaac tgggatattc agttatatga tgatggcgcc aagctgcaat
     9781 atactactga cgaagggttg acatggaatg accttggcgg aatcggtaca ggtgacaatt
     9841 ggtataatac caatacctgc tatgccatgt ggccaacgtt ctacatcgat agctataacg
     9901 gctggtcggg aaacagtggt ggctgggtgc attcaaaaca tgacctcagc ttcctggcag
     9961 gtgaaccatc tgttaaattc agatttgttt tcgcctcaag ctcttattgg aatttcaacg
    10021 acggtttcgc attcgataat atgtggatcg gcgacccgct gcctaacgat attggtgtaa
    10081 ctaatgttat ttctcctgaa acaagtcctt tcctttcagc tactgaatca gtaaccgttg
    10141 aagtgacaaa ttttggaaca aatcctcaaa caggtttccc tgttagttat caggttgatg
    10201 gtggtactat tcataccgaa acattcaccg gaacacttgg tgttgctgaa gttgctacat
    10261 tcacatttgc agctacaagc gatttaagtg ccgatggtga ttatgatttt gttgcatgga
    10321 caggtctcgc ctcagatgaa gatgttacta atgatacatg gtcagaaacg atcagcaata
    10381 tcgaaccaat cacaggcacc aacgcatatt atatctatag taattcaaca ggtttcgaac
    10441 catggtacac cacatcaaat tcacaacata tggatgatgt gtttggtgtg ggtgaatgga
    10501 ctactgaata ttatgaaaca attgatccgg cagttttatt tagcctcgac aattgcttca
    10561 tttttatgga gggttctgat tcgcacgctg atgcaatgga aactttcatc aatgcaaata
    10621 tggatgccat gcaattatgg gttgcagccg gtggacattt attaatcaat gcagcaccga
    10681 atgttggcga tggaatggat ttcggttttg atggcgtaca tcttgattat ccttggtatg
    10741 catcaaccgt ttcagcaagt gatcctactc atccggtttg ggacggacca tttgttccta
    10801 cctcaaccga tatgaccggt ttcaattacg cacatgcaag tatttcgggt gcctatttat
    10861 cacccatatt atttgatgct gcaacaccat cacgcattat ttgtgcagaa agaaattatg
    10921 gcgcaggtac tgtgattttt ggtagcatga cagttacaga ttttcataca ccgctgactg
    10981 aagcaggaaa ctttagaaag aatttgatcg cttacctcgc cacatgtacc atttcaaatg
    11041 atgatgttgg tgtgtatagc attgaatcac ctgaaacagg ttgtggcatg acagatgaag
    11101 agattgttac aatcaaagta aaaaattatg gcttcctgcc gcaaactgat gttcctgtgt
    11161 attatcaagc agatggtgga actatcgtga gcgaggtcgt gccgggaaca attggagttg
    11221 gtgatgtagt tttatatact ttcgctactc cggtcgatat ttcactcatc ggtgatcata
    11281 cccttaatgc atggacaggt ttacctgctg atacaattaa tgataacgac actacttatt
    11341 ctacacttgc aaatattccg gtaataacta catatccata ttatgaaaat tgggaattag
    11401 gagatgaaga tggttggatg gcatatggcg cttcatctac atgggaattg ggatatcccg
    11461 acggtcctgt aattaatgaa cctcctactg ctactcccgg aagtcaatat tcatggttga
    11521 caagcattac gggcaattat aatgacaatg aaaatagtta tctcgaagga ccatgttttg
    11581 atctttcatc tttagtaatt ccatatgttg aagtagatct ttggtgggaa actgaagaca
    11641 cttgggatgg aatgcaattg gaatattcta ctaatggtgg tgttacatgg tctgtattag
    11701 gtgatatcgg cacaggcgat aactggtata ccaatactgc ctttgccctc gattacgaaa
    11761 ccggctgggt aggcagcggc ggtggatggg ttacttcaca tcatgatatt tctttccttg
    11821 ccggtgaaac tgatgttaaa ttcagattta aattctctgc tgacggaata attaatttcg
    11881 atggtatagg attagataat ttcaaagtgc aagatccttg gccaaatgat gttggtgtta
    11941 ctgatttgat tactcctgtt agcggagttg atttaacaac aactgaaacg gtaagtgtca
    12001 ctgtgaaaaa ctttggaaca ttacctcaat caggtttccc tgttaatttc caagctgatg
    12061 ccggtactat tcatacagaa acatttacgg gaacattaac tgccggtgca agtgcaacat
    12121 ttacattcgc agcaactgct gatttaagtg ctgaaggatt acacgaaatt tgcgcatgga
    12181 catcgctcgc tactgatgat gatgtaacaa atgatgaaat tccgggatgt gccgatgtaa
    12241 tgcacctggc tcctgtttca ggtaccggtg cttattatat ctatagcagc acagttggtt
    12301 ttgaacccgg atggatgaat tcaaattcaa cagcaatgga tgaagtgttt ggcgatgatg
    12361 cctggacact ggattattat gaagatatag atccatatac cgttttcagt gaaagcaatt
    12421 gctttatttt cttggaagcc agtgaaggtc attggagtga atttgaatca ttcttcgaag
    12481 acaatagtga tcttattcaa ggatgggtag catctggagg taatttattt atcaatgcat
    12541 ctccttggga aggtgatggt ggtgatgttg gatttggtgg tgtaagtatt gtttacccat
    12601 actatactta tcaaacagct gcaacggatc caacacatcc gatttatttc ggaccattta
    12661 ctcctaccac ttctgacttt acaggattct atgttgctta tgcacgcact accggcgatg
    12721 taacaccaat aatgaacgat ttttattgtg atgattgcta tatcctaagt caaaaagatt
    12781 ggggcgatgg tcgcgtgttc tttggaaata tgagtgctcc ggaatattat tctccttctc
    12841 ctgataatca gaatttacgt cagaatatta ttgactatct gaagctttgt gcacctgtag
    12901 atttaggtgt tactacattg ctttctcctg acggtggttg cggaactact gcaacagaaa
    12961 cagttagcgt tatagtatca aacttcggtc cttcaaccgt tacaaatatt ccaatgaaat
    13021 tccaggttga tggcggagca attgttactg aagttgctga tggccctgtg cctatcggtg
    13081 gtacttatac gtatacattt actgcaacag ctaatctagc aactccgggt acgcatacac
    13141 ttgctacatg gacagatttc agtggtgatg aagatgaaac aaacgataca ttaatatcat
    13201 caatcgtttc actcgcaaca ccggttctgg atctcggacc aaatacaact gtgtgcgacg
    13261 atgtggtatt aaatgcatta aacgttggaa gcacatacct gtggagcacc ggtgaaacaa
    13321 cacaaacaat cacagttact acttcaggta catattccgt aactgttaca aacccgacaa
    13381 gtggatgttc aattacagat aacataacag ttactgtaaa ctatacacct gttgcaaact
    13441 tctcgtatac tgttaccggt ggaactgtaa catttaccaa cgaatctacc gatggtgcca
    13501 gttataactg gagttttggt gatggcggaa gcagcactac tgcaaatcca tctcatgctt
    13561 atgctactgc aggtatgtat accgttaccc ttactgtaac taacggttgt ggttcagatt
    13621 tctatagcgt tgttattgaa gttggaactg ccataactga tatggaactt gatcaaagtg
    13681 ttacggtata tccaaacccg acagatggtc gcaccaatgt aaatattgct ctcagcgaag
    13741 cgcagaatat taccttggaa ctgttgaacc aaatcggtca gactgtttgg acaaataatc
    13801 ctggtaacat tttgactgaa agtatcaata ttgatatgac gaatatggct gccggagtct
    13861 ataacctgaa agttacaggc gataatggaa ctgccactaa acaaatcata ttgaccaaat
    13921 aaaaagggaa accttcatca aaggccggag ttttacttcg gccttttttg tgtacttttt
    13981 gttaaattgt tgataaataa gggctcgtct aacttattat acattttttt acgctttttt
    14041 ctttgaagta cacttttcta gggcgtatct tagtccgcaa agattgtcag ccaaaacaga
    14101 cttttatgaa aaaacacctt acgcttctcc tcagttgtat gctaacgctc gcctttatgg
    14161 ctccgacgcg tgatgctatc gccggtggtg ataacaacta ctacatctac tcgaatgcct
    14221 acggtggtta cgaaccctgg tacactactt ccaattccga tgctatgaac gcagtattcg
    14281 gtgttggcgg ttggttacaa gattacttcg aaacctgcga tccggcattg gtattttccg
    14341 caaacacaaa cttcgtattc ctggaaggaa gcgatggctt cgccgatgag ctggagtcat
    14401 tcctctctgt gaatattgat gtcattgaaa cttgggttga aggtggtgga cgactacttc
    14461 tcaacgctgc accaaatgag ggcgacggta tgtcgttcgg atttggaggg gtaaacctca
    14521 cctactgggg aggttcgagc aacgtgcatg ctaccgaccc tacgcacccg attttcaacg
    14581 gaccgtacac ccctaccgga acgtcatgga caggttcttc cttcagccat gccagcgttt
    14641 ccggtccgga tcttacaccg ctgatcatcg acgactttac accaactaca attgcgcttt
    14701 ctgaaggtcg ctatggtact gatggtattg tgttctttgg cggaatgact accgataact
    14761 tccattatcc atcattagaa tctatcaacc tctttcaaaa tatcctcaac tatctcgcat
    14821 tctatgagat acctgatcat gatattgcct ctctttctct tgccagtcca tcttcaggat
    14881 gcggcatggg cgtggaaacc gtgagtgtca actataaaaa ttatggcacg aatgatgaag
    14941 taaatattcc ggttagttat cagcttgatg gcggcgcagt ggtttcagct acaattgccg
    15001 gtccggttgc tcccggtgaa actgtaacct ataattttgc cgtgcccgca gatattactg
    15061 ccttaggagc gcataccttc gtgatctcct gcgacctggc aacagatgaa atacctgcaa
    15121 ataatacaat tacggttacc attaataata ttcctgttgt tgacgtattt ccatactatc
    15181 aggattttga agccggcgac ggtggatgga tcgcaggtgg aggcgctagc tcatgggaac
    15241 tcggaagccc tgcaggtttc gcaatcgttg gcccccctcc tgctactccg ggtagtgtga
    15301 atagctggac aacaagcctt acaggatact ataatgatgg cgaaaaatca tatatcgaat
    15361 caccatgttt cgatttttca tcacttgttt ttccttacct tgaacttgac ctgaactggg
    15421 atactcaaat gtattccgat ggtgccaaaa tccagtattc aactgatgca ggcgccactt
    15481 ggagcgatct tggcgatgta ggtaccggtg aaaactggta taccagctat aactgctatg
    15541 gtatgtggcc tgatttctat gttaccgatt accgcggctg ggatggcaat agcggtggat
    15601 ggcaacatgg ctaccaagat cttgctttcc tcggcggtca gccctcagtg aaattgcgca
    15661 tggttatggg cgccgatctt tattggagct attatgatgg ttttgcattt gataacgtaa
    15721 aaatatcaga cctcttccct aacgatgttg gagtttctaa catcacagca cctgtttctg
    15781 gacctctgct gtcagctacc gaatctgttt cagtagctgt tactaactat ggaacacttg
    15841 ctcagtcagg attccctgtt tcttatcgct ttgaaggcgg ccctgttcat acagaaacat
    15901 tcacaggaac tgtaaacccg ggtgaaactg ttaactatga tttcgcaact actgaagacc
    15961 tcagcgttct cggagattat accttcgaag cctggacttc attagccacc gatgaagaca
    16021 tgtctaacga ttcaatcacc gaagttatct ccaacctcga gccacttacc ggtgataatg
    16081 cctattatat catttctaac accacaggcg gtgaaccttg gtatactgtg agcaactcac
    16141 aatccatgga tgccgttttc ggtgttggtg aatggaccat gggctacttc
//
LOCUS       DAOQOU010000056        16071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1833_length_16071_cov_5.854021, whole genome shotgun sequence.
ACCESSION   DAOQOU010000056 DAOQOU010000000
VERSION     DAOQOU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1833_length_16071_cov_5.854021"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /gene="gldM"
                     /locus_tag="PLM90_06655"
     CDS             <1..1597
                     /gene="gldM"
                     /locus_tag="PLM90_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HMZ89087.1"
                     /translation="PKEPRQLMINLMYLVLTALLALNVSAEILNAFNTVNNGISSSNE
                     LLTSKNELMMTMISKAAAKDARPETQEILRQATEAKKMSDEFVKYVEGLKEEVKARAG
                     GPNPDHPEKLKTEGEMEKTSRLFLNEGKGKELEAKINDAREKFLNVLGKESGIDVPLR
                     IVEVPEGEHATNWEEYNFDRVPAIAVMTILTKFQQDAKSSESTILDYLRRKINAEDIQ
                     VDKMAAMVVAPSSYVRKGSEYTADIFIGATSKNIKPEVYVGTFTSAVKKDPADPTGTA
                     FLEVEGKDIPLNGARPIDMVNGIGKIKEGANGQRNFQGVIKIPHPSKEGEFRFYPFEA
                     GYETFEVGKAVISPTAMNVLYIGVDNPIKISVPGYASEKVSASGCGVTKVKGEEYVAR
                     PSKVGEEEIVVSVQGNKGVEKSSEKFRIRRVPDPYVYILNTKGGPMKIGEFKAADGII
                     VKNPDFVFQVPYTVTGFEMLYAPKGSGGVSDMSNSSKFSSTMKDIQKKCKPGDTIVLN
                     DVAVKMPDGRTVKISASFKLIGG"
     gene            1618..2448
                     /gene="gldN"
                     /locus_tag="PLM90_06660"
     CDS             1618..2448
                     /gene="gldN"
                     /locus_tag="PLM90_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HMZ89088.1"
                     /translation="MNRTLLILGLVLVLGTAVHAQPTSPAKLDGIYKRELLGTREIIP
                     YDDIREADIYWEKRIWRNIDFREKINLPFTWPVDPFVQVVYDAVMSGELTAYSPLYDD
                     FREGTAIPTDELKARFNKVDTTWILDETTYEEVPVIVVNEFDYKTVQKIQIKEDWVFD
                     EETSTMVVRIIGLSMIRDRIDPTTGEVITTETMFWLYYPDLRNVLIRHEVFNDKNSAR
                     YLTYEDIFEMRLFNSVVVKEDNVYDRNIESYATGIDQVLESERIKQQIFEFEHTLWEF
                     "
     gene            complement(2512..4326)
                     /gene="uvrC"
                     /locus_tag="PLM90_06665"
     CDS             complement(2512..4326)
                     /gene="uvrC"
                     /locus_tag="PLM90_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMZ89089.1"
                     /translation="MDLDAFKQIEINIPGNPGVYRYYDKEGTLLYVGKAKNLKKRVSS
                     YFQKTDHTARIKLMVKKIAKIEFTIVNNEADAFLLENTLIKKHQPRYNVNLKDGKTYP
                     YIVVKNERFPRIFLTRQKRQDGATYLGPYTSVGKVKTVFDFIKSVFPIRTCNFHLSEQ
                     NIQAKKFKVCLEYHLGNCKGPCEGLMQEADYNENVQQIMHILKGNVGSVKVAFKKQMQ
                     QYVEQLQFEQAELMRKKLESVEAFQGRSTVVNTSISNVDVFGYTDAESFAVIGYMKIM
                     QGMVTQATTLVLTRKLDETPEELLQIAVMELRLQFESDTPEIIVPFQIEWIDEKLVQT
                     VPQAGDKKKLLELAFKNAMYVREEKEAGKQKREDRNQNTRVLEAIKADFKLTQLPYHI
                     ECFDNSNIQGTNPVASMVCFKNAKPSKKDYRHFNIKTVEGPNDFASMHEIVHRRYKRL
                     IEEAIPLPQLIVIDGGKGQLGAAVDALKELGIYGQVAICSIAKRLEEIYFPEDPLPLY
                     INKKSESLRVIQQLRDEAHRFAITFHRQKRSKSAIKSELDDIKGIGRATASNLLKAFK
                     SVKNIRNASIEALSEVVGQAKAQIIIDHFHTTGESSKA"
     gene            complement(4429..6726)
                     /locus_tag="PLM90_06670"
     CDS             complement(4429..6726)
                     /locus_tag="PLM90_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ89090.1"
                     /translation="MSSTTIKEQFSTYKRFIRWFWIIFGSGSLLVIIFILLLSAGVFG
                     ALPSFEELENPQSNLATQILTSDGELLGRFYRENRTNATYDEIPPFLIDALVSTEDRR
                     FYEHSGIDAKSVMRVIFKTILLHQEESGGGSTLTQQLAKNLYGRPVASTKFELGLIKF
                     KEWITAAKLERRYTKEEIIVMYLNTIPYIYDASGVKTAAKTFFNKSIQDISVEEAAVL
                     VGMVQNPSFYNPVRFPDRCRDRRNIVLHEMHEANKLSDEDYQKAKESPLVLNFKRQDH
                     KAGSGTYFREYLRQWLADWVEENPKPDGGKWDIYKDGLKIYTTIDSRMQAYAEQASQE
                     WLTKLQTQFDNHWKGNKYEPWAYPYSGRTNPELINKAMKQTDRYRGLKKAGKTDAEID
                     KIFHTPDSMTVFSWKSKNYDLDTIMSPYDSIKYVKKMLQPGFMAMDPHTGGIKAWVGG
                     IDMRYFQLDHVKPSHGRQVGSTFKPFVYTAAIADKGFSPCYQIPNLPVSFEKGEKWGL
                     LSDWTPKNSDGHYGGMLTLSEGLGKSVNTVTAFLLREVGIQPVIDLSKNMGIESEIPY
                     QPSICLGTPEVTVYEMTAAYSAFANKGVAVRPVFVTRIEDKNGNIIAEFPAKRVEVMR
                     EEVASVMLNMMEYVVNSGTGQRIRRPEYGGITSEVAGKTGTTQENTDGWFMGMVPQLV
                     CGTWVGADDPVVHFRSTALGQGANMALPIYGLFMKRVYADKTLGIDPNAHFPAYEGTR
                     TIELDCGKYRDNQRNDYQDLMEGYN"
     gene            complement(6835..9351)
                     /locus_tag="PLM90_06675"
     CDS             complement(6835..9351)
                     /locus_tag="PLM90_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89091.1"
                     /translation="MTRSVDDLIARDNGYFNARLIMRESEKNLWEEQQDNYEEILPIF
                     KYGTQEQATAIQPGMDEVNTKCSWVIQKHKKSKWVDDCYFLLGKAYFYERNYDEALTS
                     FQYIVAEYSNLIEKQSKNKRISEDEDGELSFFEKLKHQPVSSEAGIWVARSLVEKKKF
                     SEAHTVISVLKSKENFPKWLLGELYAVEADGYMKQGQVENAVEPLSEAVANTEDKVLK
                     ARYLFILAQLYAQQHDTKNAMKAYQEVLQLKPSYDMDFYTRLNMAKLSLENSSLSGRE
                     SKEDLLALLKDEKYKDYYGLIYYTLADIAFADKDQDMGMQYLNLCIRSTNDGKQRGLA
                     YMRLGDIYFSEPEYKTAYAYYDSTVNTLPSEHERFKEIKSLRDNLKLLVDELTIIETE
                     RKLQYWAGLSDKDLMKELEKLVPEVEEKDSLEANVTNPNNVTPLTPGITDGDFYFYNT
                     TLRSRGFSEFKKTWGTRKLEDNWRRSEKGSFGEEGETAVEGTGEEQSIDLTGKNITID
                     KIKQSLPTTPEMIAQSNARIAGALYRAGMIYKDNFRDKAKAIGSFEDNVNNFEGNEYE
                     IHCLYQLYRLTSAPAQNNYKQKLLDKYPESDYARIITDPGFYERKEAEKHALEDYYAV
                     TYDLLQKRDFAGVKSRVVAADSLFKVNPLKPKFDMLNALADADSLDLFKNSLQKVALK
                     YPEHEVGVRAQEILDYLRRGSALEDAGKGIVTANYTHDATEEHYFIYVMSGTGKNATT
                     MKNNVATFNSINFSSDNLKISSLLLGKENSIILVKSFNNQDKAMIYYYAIKDNPGVYK
                     DLAKDGFLPVVISKTNYVQFFKVKDVEGYEAYFQENYLDK"
     gene            9599..10411
                     /locus_tag="PLM90_06680"
     CDS             9599..10411
                     /locus_tag="PLM90_06680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ89092.1"
                     /translation="MHDKTFEVQASLRLSPLNVIILSSTLFVVIGLIVYMAIVFTPLK
                     NLVVGYSEVTTSRQIAENKRVTDSMLTTLQAYDMYFNNLQKRLRGETDSIIPIDSLKK
                     TDYSKIVVDEAGETDLAMREQIEREDLFNLSISDPRETTGAQNLESLHFFPPIKGTVT
                     SNFQPSNKHFGIDVVAPENTPVKACLDGVVIDSYWNFESGNVVVIQHDHGLISFYKHN
                     SQILKRSGNKVRAGDVIAIIGNTGELSSGPHLHFELWHDKTPLDPKEFINFN"
     gene            10426..10842
                     /locus_tag="PLM90_06685"
     CDS             10426..10842
                     /locus_tag="PLM90_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMZ89093.1"
                     /translation="MFANKTNTPQSTPGSYISNRINHGTVMEGKLDSDGDIRVEGIIR
                     GSIRTSAKVAVGSTGLIEGDIHCKSADIEGRIIGDVEVTEVLTLKSTAIVEGNIYTAK
                     IVIENGAHFDGICNMGTKEKERRITHAQPELEEAAI"
     gene            10850..11035
                     /locus_tag="PLM90_06690"
     CDS             10850..11035
                     /locus_tag="PLM90_06690"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HMZ89094.1"
                     /translation="MKYSGMAFQLFAGIFLGVWGGIKLDAWLNTKPWFTVILSLFGIA
                     AGMYSVLKDFIDSGKKK"
     gene            11065..11445
                     /locus_tag="PLM90_06695"
     CDS             11065..11445
                     /locus_tag="PLM90_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89095.1"
                     /translation="MIIFAVVLAIVLLILGMVSEFKPAMTFAWMCFGMFFALTLITYF
                     FSAKTMERKFSSFMNVFFVGIFSKLILSAIMVLIFKVKNETTGLNYIVPFAIIYFTFL
                     FFETVELVKLSRSIGNPPPQKPTK"
     gene            11447..13387
                     /locus_tag="PLM90_06700"
     CDS             11447..13387
                     /locus_tag="PLM90_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMZ89096.1"
                     /translation="MRIPGFLLLLCSPFFIKAQTPDLHAFEKSMVFAEQQAAAGKISV
                     QSTAPTNNYDLIYQKCAWTIDPDTLFIDGTITSQLVLTEESNAIYFDISDSLKVDAVT
                     VNGVEGDFLTANNAVQINLDDTYDIGAEFEIAVTYHGTPSTNGFGSFKKTSQDAGNVI
                     WTLSEPYGASDWWPCKQSLTDKLDSVDVWITMPDNCKSGGPGLLDTITEAGIGWHTYR
                     WKHRYPIATYLIGISVTDFDEFSFYAPHHEDSVLFYNLVYPSSFDEAVTQINGIVPAF
                     EIFSDIYGDYPFADEKYGHMQFGWGGGMEHQTMSSVSGYSHELLVHEMAHQWFGDKIT
                     CASWHDIWLNEGFATYSTWLSYDLGDDPNNYYEAWLKTTRDKVVSLPDGSVYVDDTTL
                     VSRIFDGRLTYYKGGWVLHMLRWELGDDAFFAGIKNYITDPAFSFKFVTTQDLISHLE
                     EAADTSLTEYFDDWFYGQGFPEFQIIWQQDTLGHFIMSVSQTPTHESVDFFNMHVPLR
                     LYGNGDSLDLRLNHTVNGELFEMDLPFMVDSIVLDPELRILHANDAIVHLPITEGILQ
                     AVIYPNPVSDILHMQILEPSASTYNLTIWNAAAEKVVSAEFNTSGSSASYTIDSTSWP
                     SGLYMVLIKSGEKEVVQSVVVQ"
     gene            complement(13388..14626)
                     /locus_tag="PLM90_06705"
     CDS             complement(13388..14626)
                     /locus_tag="PLM90_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ89097.1"
                     /translation="MLACLHGLIQAQQNFQPAIIFSKTGDTIPGYIDYRQWDKSPETI
                     HFRQNEESPTINMNPLTIAGFSVSGECYISSVIGLDGTPYTYRELPRTITQKIIIDTV
                     FLKTLVLSDGGMDLYSLKDSRGKQHYFIHDSGSMTELIDIKYYSESEQSGIVMHVYKD
                     QLYEWMEECPPAAKASLDADLKTDDLIEAITNFDSCLAVPIDYISPILPLKKVFGLAI
                     GYGINRVTYPISMVSTGYGTADFNWGPTYYGALFYDIDMASNRESFWLCFDGGIAQNK
                     SSGTYFGEIGNSHESGSFTFSALETLVSTQFKWQSNAPHFKPYLKAGYLATFEIQQSN
                     SMHTEKYNIYVPDVVTVTDEENSINYRKFANHIVGQTGLSINNKYFIELTYSTGLTKG
                     ADDFVSINRDRLTLGLKYGI"
     gene            14719..15882
                     /gene="purT"
                     /locus_tag="PLM90_06710"
     CDS             14719..15882
                     /gene="purT"
                     /locus_tag="PLM90_06710"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HMZ89098.1"
                     /translation="MKKIMLLGSGELGKEFVIACKRLGHYVIAVDSYNDAPAQQVADT
                     REVISMLDGDALDAIVKKHEPDIIVPEIEAIRTERFYDYEKWGIQVVPSAKAANFTMN
                     RKAIRDLASKELGLRTAKYAYALSFEELKTAVAEIGIPCVVKPLMSSSGKGQSVIKTE
                     ADIQTSWDYAQAGKRGDIAEVIVEEFIRFDSEITLLTVTQKNGPTLFCPPIGHRQERG
                     DYQESWQPALIKPEHLLEAQHVAKQVTEALTGAGIWGVEFFLAETGVYFSELSPRPHD
                     TGMVTLAGTQNLSEFELHARAVLGYPVPEITLERAGASAVILAEKESNSFSIVGVEKA
                     MQEVNTDVRIFGKPTTRKYRRMGVALACGKPDTNTDALRKKAMINASKVEIKY"
BASE COUNT         4543 a         3266 c         3326 g         4936 t
ORIGIN      
        1 acctaaggaa ccacgccagc tcatgatcaa cctcatgtac ctggtgctta cagccctttt
       61 ggcattgaac gtttctgcgg aaattctgaa tgcttttaat actgtgaaca acggtattag
      121 ctccagtaac gaactcctca ccagcaaaaa tgagttgatg atgactatga tatctaaggc
      181 tgctgccaaa gatgctcgtc ctgaaactca agaaatcctt cgccaggcaa ctgaagcgaa
      241 gaaaatgagc gatgagtttg taaagtatgt agaaggtttg aaagaagagg ttaaagctcg
      301 cgccggcggt ccaaatcctg atcatcctga aaaattaaaa actgaaggtg aaatggagaa
      361 aacaagccgc ctgttcctca atgaaggaaa aggtaaagag cttgaggcta aaatcaatga
      421 cgcacgtgag aaattcttga atgtgcttgg taaagaaagt ggtatcgatg tgccactgcg
      481 tatcgtagaa gtacctgaag gggaacatgc tacgaactgg gaagaatata atttcgatcg
      541 cgttcctgct atcgctgtta tgaccatcct taccaagttt caacaagatg cgaaatcatc
      601 tgaatcaacc atcctcgact acctccgccg taaaatcaat gcagaagaca ttcaagttga
      661 taaaatggct gccatggttg tagctcctag ctcttatgtt cgcaagggct ccgaatatac
      721 tgctgacatt tttatcggag caacttctaa gaacattaaa ccggaagtgt acgtgggaac
      781 tttcacaagt gcagtgaaaa aagacccagc cgaccctaca ggaacagcgt ttcttgaggt
      841 ggaaggaaaa gacattccac tgaatggcgc tcgtccaatc gatatggtta atggtatcgg
      901 taaaatcaaa gaaggcgcta acggacaacg caatttccaa ggcgttatca agattcctca
      961 cccgtctaaa gaaggtgaat tccgcttcta tccttttgaa gccggttacg aaactttcga
     1021 agtgggtaag gccgttatct ctcctacagc aatgaacgta ttatatattg gtgtagataa
     1081 tcctatcaaa atttccgtac cgggttatgc ttctgaaaaa gtaagcgcat caggttgcgg
     1141 tgttaccaaa gtgaaaggcg aagaatatgt tgctcgccct tcaaaagttg gtgaagaaga
     1201 aatcgtagta agcgttcaag gaaacaaagg cgttgaaaaa tcttctgaga aattcagaat
     1261 tcgccgtgtg cctgatccat atgtatacat cctgaatact aaaggtggtc caatgaagat
     1321 cggtgaattt aaagccgctg atggtatcat tgtgaaaaac cctgacttcg tattccaggt
     1381 gccttataca gttaccggtt tcgaaatgct ctacgctccg aaaggtagtg gcggtgtttc
     1441 tgatatgtct aattcgtcta aattctctag cacaatgaaa gacattcaga aaaaatgcaa
     1501 acccggcgat actatcgtgt tgaacgatgt tgccgtgaaa atgcctgacg gtcgtacagt
     1561 aaaaattagc gcctcattta aattgattgg cggttaatct taagttgaat aaaaggtatg
     1621 aatagaacat tgttaattct cggtttggta ctggtacttg gtactgccgt acatgctcag
     1681 ccaaccagcc ctgcaaagct ggatggcatc tataagcgtg aactgctcgg tacacgtgag
     1741 atcattcctt acgatgatat ccgcgaagct gatatctatt gggagaaaag gatctggcgt
     1801 aatatcgact tccgtgaaaa gatcaatttg ccatttacct ggcccgtaga tcctttcgta
     1861 caagttgtgt atgatgcggt tatgagtggt gaactcactg cgtatagccc attgtatgac
     1921 gactttcgcg aaggaactgc catccctacg gatgaactca aagcgcgctt caataaagtg
     1981 gataccacat ggatcctcga tgaaacaact tatgaggagg tgccggtaat cgtagtgaac
     2041 gagttcgatt ataagaccgt tcagaaaata cagattaaag aagactgggt attcgatgaa
     2101 gaaacttcca caatggtggt acgcatcatc ggactctcca tgattcgcga tcgtattgac
     2161 cctacaacag gtgaagtgat cactaccgaa acaatgttct ggttatacta tccggatctg
     2221 cgtaacgtgc tgatacgtca tgaagtattt aatgacaaaa acagcgctcg ttacctgacc
     2281 tatgaagata tcttcgaaat gagattgttc aacagcgtag tcgttaaaga agataacgtt
     2341 tatgatcgca atatcgaaag ctatgctacc ggtatcgatc aggtgttgga aagcgaaaga
     2401 atcaaacaac agatcttcga attcgaacac acgctctggg aattttaatt accttgcttt
     2461 tctattcata cttgaaccga aggccgatct aataagtcgg ccttcacttt tttatgcctt
     2521 cgatgactca ccggtggtat gaaaatgatc aatgatgatc tgtgccttcg cctgacctac
     2581 aacttcgctt agcgcttcaa tgcttgcatt gcgaatattc tttaccgact taaaggcctt
     2641 caataaatta ctcgccgtag cccggccaat tcctttaatg tcgtcaagct ctgatttaat
     2701 agccgactta cttcgttttt gtctgtgaaa agtgatggca aaacgatggg cttcatcgcg
     2761 aagctgttgt attacccgca aactctcact ctttttattg atatacaagg gtaggggatc
     2821 ctccgggaaa tatatttctt caagtcgttt tgcaatactg caaattgcta cctgtccata
     2881 aatgccaagt tcttttaagg cgtctactgc ggcgcctaat tgtcctttac caccatcaat
     2941 aacaataagt tgtggcaatg ggatggcctc ctctatcaga cgcttgtatc gacgatgaac
     3001 gatctcatgc atgctggcaa aatcgttggg tccttcaact gttttgatat tgaaatgacg
     3061 gtaatctttt ttactgggct tggcattttt aaaacatacc atactcgcaa ccggattggt
     3121 tccctggata tttgagttgt caaaacattc gatatgatat ggtaattgag tgagtttgaa
     3181 atcggctttg atagcttcaa gtactcgtgt attctggttg cgatcttcgc gcttttgctt
     3241 tcccgcttcc ttttcctcgc gcacatacat cgcatttttg aatgcaagct ctaataattt
     3301 ctttttatcg ccggcttgag gaacggtttg cactaatttt tcatctatcc attcaatttg
     3361 aaacggaaca atgatttcag gtgtgtcact ttcaaattgt aaacgcaatt ccataactgc
     3421 aatttgaagt aattcctccg gcgtttcatc tagttttctg gtgagcacta aggttgttgc
     3481 ctgtgttacc atgccctgca tgatcttcat atatccgatt acggcgaaag attcggcatc
     3541 tgtatagccg aacacatcta cattgctaat gcttgtattc acaacggtgc tgcgcccctg
     3601 gaaggcttct acactctcca gtttttttcg cattaattca gcttgctcaa attgcaattg
     3661 ttctacatat tgctgcatct gttttttaaa tgcaacttta acagatccga catttccttt
     3721 gaggatgtgc atgatctgtt gcacattttc attgtaatca gcttcctgca tcaacccttc
     3781 acatggaccc ttacaatttc ccaggtgata ctcgagacat actttaaatt ttttcgcctg
     3841 aatattttgt tcactcaaat gaaagttgca agtgcgaatc ggaaatacag attttatgaa
     3901 gtcaaaaact gttttcacct tgccaacaga ggtataaggt cctaaataag tagcgccatc
     3961 ttgtcgcttt tggcgtgtaa ggaaaatgcg cgggaatcgc tcgtttttta caacgatata
     4021 tggatacgtt tttccgtcct ttaaattgac attgtatctt ggttgatgtt ttttaatcag
     4081 ggtgttttcc aatagaaagg catcggcctc attattcaca atagtgaatt cgatctttgc
     4141 gatctttttc accatgagtt ttatccttgc cgtatgatcg gtcttttgaa aatacgacga
     4201 aacgcgcttc ttgagatttt ttgccttccc cacatataac aaggtgcctt ccttgtcgta
     4261 ataacgatat acgccgggat ttcccgggat atttatttct atttgtttga aggcatcaag
     4321 atccataggt ggtcggtcgg ctgcataaag ttaatcgtaa acggcaaaaa aaaatcgccg
     4381 acagcaaaag taaactgtcg gcgatgagtt tattgcttcg ctattatgct aattatatcc
     4441 ttccattagg tcctggtaat cattgcgctg attgtcgcga tatttaccac aatccaattc
     4501 aatggttcgt gtgccttcgt atgcagggaa atgtgcattg gggtctatac ctaatgtttt
     4561 atcggcatag acgcgtttca taaataaacc atatatcggc aatgccatat tcgcgccttg
     4621 ccccaatgcc gtgcttctga aatggacaac cgggtcatca gcacctaccc aggttccgca
     4681 aacgagttgt ggtaccattc ccataaacca tccatcggta ttttcttgtg tagttcctgt
     4741 tttccctgca acctcgctgg taatgcctcc atattccgga cggcgaatgc gctgtcctgt
     4801 accgctattt actacatact ccatcatatt gagcataacg gatgccactt cttcgcgcat
     4861 cacttctacg cgtttggcag gaaattctgc aatgatattg ccgtttttat cttcaatacg
     4921 tgttacaaaa acaggtcgca cagcaacgcc tttatttgca aaagcactat aagcggcagt
     4981 catttcataa acagttactt ccggtgtgcc taaacaaatg gaaggctgat atggaatttc
     5041 cgattctatg cccatgtttt tagaaagatc aataaccggt tgaattccca cttctcgcaa
     5101 taaaaatgca gtaacggtat tcaccgattt tcctaatcct tcgctaagcg taagcatgcc
     5161 tccataatga ccgtcagagt tttttggcgt ccagtcgctg aggagtcccc atttttcgcc
     5221 cttctcaaat gatacaggga gattaggaat ttgatagcag ggagaaaatc ctttatctgc
     5281 aatagccgcc gtatatacga aaggtttaaa tgttgaacct acctggcgac cgtgagaagg
     5341 ttttacgtga tcgagttgga aatagcgcat atcaatacca cctacccatg ctttgatgcc
     5401 acctgtatga ggatccattg ccataaatcc gggttgaagc attttcttta catacttgat
     5461 ggaatcgtac ggcgacatta tcgtatcgag gtcataattc ttgctcttcc aggaaaatac
     5521 tgtcatcgaa tccggagtgt gaaaaatctt atcgatttca gcatccgttt tccctgcttt
     5581 ttttaatccg cgatagcgat cggtttgttt catcgccttg ttaatcaatt ccggattagt
     5641 tctgccgctg taaggataag cccaaggctc atatttattt cccttccagt gattgtcgaa
     5701 ttgcgtttgc aatttggtga gccactcctg cgatgcctgc tccgcatatg cttgcattcg
     5761 gctatcaatg gtggtgtaaa tttttaatcc gtccttataa atatcccatt tcccaccatc
     5821 gggttttgga ttttcttcca cccaatctgc cagccattga cgtaaatatt cgcggaaata
     5881 agttccactt cctgctttat gatcctgacg tttgaaattt aataccagtg gcgactcttt
     5941 tgccttttga taatcttcat ccgataattt attggcttca tgcatttcat gcaatacaat
     6001 atttcttcgg tcgcgacaac gatcaggaaa gcgcacagga ttgtagaatg aaggattctg
     6061 caccatccct acaagtacgg cggcctcttc tacagaaata tcttgaatag atttgttgaa
     6121 gaacgttttg gcagcggttt ttacaccact cgcatcgtag atatacggaa tggtatttag
     6181 atacattaca ataatctctt ctttcgtata ccgacgttct aattttgctg ccgtgatcca
     6241 ttctttgaat ttgataagcc ctaattcaaa ttttgtagat gcaacaggtc tcccatataa
     6301 gttttttgcc agctgctgag taagtgtact acctccaccg gattcttcct ggtgtaacag
     6361 tattgtttta aatatcacac gcatcacact ctttgcatct ataccgctat gttcatagaa
     6421 cctgcgatct tctgtagata caagtgcatc aatcaggaat ggcggtatct catcataggt
     6481 ggcattggtt cggttctcgc gatagaatcg tcctagcagt tcaccgtcgc tcgtgagaat
     6541 ttgcgtggca agattactct gcggattttc caattcctca aaggaaggca atgcaccaaa
     6601 aactcctgcc gagagtaata aaataaaaat aatgaccaat aatgaccctg agccaaaaat
     6661 gatccagaac caacgaataa atcgtttgta ggtcgaaaat tgctccttga tggtagttga
     6721 ggacatgcct gtaagttaaa ttgtgattgg agggacaaac tctaaattac aaaagtatcg
     6781 ctgttgccat actgaaacgc ggtatgcttc cacaaattaa atggggaatt ccaattattt
     6841 gtccagatag ttttcctgga agtaagcctc atagccttct acatctttaa ccttaaagaa
     6901 ttgaacataa ttagtcttag agatcacgac aggaaggaat ccgtctttag ccagatcttt
     6961 atatacaccc ggattgtctt tgatggcata ataatagatc attgctttat cctgattgtt
     7021 gaaggatttt acaagtatga tactattttc cttgcccaat aaaaggctac tgatttttaa
     7081 attatcagag ctgaaattaa tactgttgaa ggtggcaacg ttattcttca tggtggtagc
     7141 atttttacct gttccactca tgacataaat gaaataatgt tcctctgtag cgtcgtgggt
     7201 gtaattggct gtaacaattc cctttccggc atcctccaaa gcgctgccgc gacgaaggta
     7261 gtccagtatt tcctgtgcac gaacgcctac ttcgtgttca ggatatttta aagcaacttt
     7321 ttgaaggcta tttttaaata aatccaagct atctgcatca gctaatgcat tgagcatatc
     7381 aaatttaggc ttgagaggat ttactttgaa taaactatct gcagcaacta cgcgtgattt
     7441 tacacctgcg aaatcacgtt tttgtaaaag atcatatgtt actgcgtagt agtcttctaa
     7501 ggcatgtttt tctgcttcct ttcgctcata gaaacccgga tcggtgataa tgcgtgcata
     7561 gtctgattcg ggatatttat ctaaaagttt ttgcttgtaa ttgttttgtg caggagccga
     7621 cgttaatcga taaagttgat atagacaatg tatttcatat tcgttgccct cgaaattatt
     7681 gacattatcc tcaaatgatc ctatcgcttt agccttatca cggaaattat ccttgtaaat
     7741 catgccggca cgataaagtg cacctgcaat ccgagcatta ctttgagcga tcatttcggg
     7801 agtggttggt aaactctgct tgatcttatc aattgtaata ttttttcctg tcaggtcgat
     7861 tgattgttct tctcctgttc cttcaactgc tgtttcacct tcttcaccaa agctgccttt
     7921 ttcgcttctt cgccagttgt cttccaattt tcttgtgccc caggtttttt tgaattctga
     7981 aaatcctcgg gagcgcaggg tagtgttata aaaataaaaa tcaccatccg tgatgcccgg
     8041 tgtaagaggt gttacattat taggatttgt aacatttgct tccaggctgt ctttttcttc
     8101 aacctccgga actaacttct ccaattcttt cattaaatct ttatcggaaa gtccggccca
     8161 gtattgaagt ttgcgctcag tttcaataat agttaactcg tctaccaata actttaaatt
     8221 gtcgcgaagt gattttattt ctttaaagcg ttcatgttca ctcggcaaag tatttacggt
     8281 gctgtcgtaa taggcgtatg ccgttttgta ttccggttcg ctgaaataaa tatcgcctaa
     8341 acgcatatag gctaatccgc gctgctttcc atcattggta ctgcgaatac atagattcaa
     8401 atattgcata cccatatcct gatccttatc ggcaaaagca atatcagcta gagtataata
     8461 tattagtcca taataatctt tatatttttc atcttttagc aatgcgagta aatcttcttt
     8521 tgattcacga ccgcttaaag agctattttc aagactcaac tttgccatat tgaggcgagt
     8581 ataaaaatcc atatcatagg aaggctttaa ttgcaaaact tcctgatagg ctttcatggc
     8641 atttttggta tcgtgttgtt gagcgtacag ttgtgcaagg ataaacaagt agcgcgcctt
     8701 caatactttg tcttcggtgt tggcaacagc ttctgataaa ggttcaacgg cattttccac
     8761 ttgtccttgt ttcatgtagc catcagcttc aacggcgtat aattctccca ataaccattt
     8821 ggggaaattc tccttgcttt tcaatacgct gataacggta tgtgcttcac tgaatttctt
     8881 tttctcaact agtgagcggg caacccatat tccggcttcg ctggatacgg gttggtgttt
     8941 gagtttttca aaaaaactta attcgccatc ctcatcctca gaaatacgct tgttttttga
     9001 ttgcttttca atgaggttgg aatattcggc aacgatatat tgaaatgaag taagcgcctc
     9061 atcataattg cgttcgtaga agtaggcctt gcccagcaga aagtaacaat catctaccca
     9121 cttgcttttt ttatgttttt gaatgaccca cgaacactta gtattcacct catccatgcc
     9181 cggttgtatg gcagtagcct gctcctgggt accatacttg aaaatgggga gtatctcttc
     9241 gtagttatcc tgctgttcct cccataggtt cttctcagac tcgcgcatga tcaggcgggc
     9301 gttgaaatac ccattgtcgc gagctatgag gtcatcaaca ctccgcgtca cgatatttcg
     9361 gtcggcattt ttactcgaac tgcaagccgt aacgaaaaaa agaagtaaaa ttgtgactgg
     9421 aattaggact gaggatcgtt tcaaggttac tagttttata ccggaataat taactctatt
     9481 acggcaaaat tattttaatt aaagcgaaaa ttacagcatt tcagcgtcaa tggccaaagg
     9541 gggggtgaaa actaacaaat tatgggaccg gatacggacg aaataccgac tggttttgat
     9601 gcacgacaag acctttgaag tgcaggcctc gctgcgtttg agccccttga acgtgatcat
     9661 tctttccagc acactttttg tagtgattgg ccttattgtt tacatggcaa tagtcttcac
     9721 cccgctgaag aacctcgttg tgggctatag tgaggtcaca acttcgagac aaatagctga
     9781 gaataagcga gttacggata gtatgctcac tactttgcag gcttatgaca tgtattttaa
     9841 caacctccag aagcgtctga gaggtgaaac ggatagcata atacctatcg atagcctcaa
     9901 aaaaacagat tatagcaaga ttgtggtaga tgaggccggt gaaacggatt tggctatgcg
     9961 cgagcaaatt gaaagggaag accttttcaa tctcagcatt tcggaccctc gtgagactac
    10021 aggtgctcaa aatttggaat cgctgcactt tttccctccc atcaaaggaa ccgttacctc
    10081 aaacttccag ccctcaaaca agcattttgg tattgatgtg gtggctcctg aaaacacccc
    10141 tgtaaaggcc tgtttagatg gggtggtaat agacagctat tggaattttg agagcggtaa
    10201 tgtagtggtg atacagcatg atcatgggct catttcattt tataaacaca acagtcagat
    10261 actcaagcgc tcaggtaata aggtacgtgc cggagatgta atcgccatca ttggaaacac
    10321 gggtgaattg agcagcggcc ctcatttgca ctttgaactt tggcacgaca aaactcctct
    10381 tgatccgaaa gaattcatta acttcaacta aaatttcata gccttatgtt tgctaacaaa
    10441 acgaacaccc cccagtccac accagggagc tatatttcaa acaggatcaa ccatggtacc
    10501 gtgatggaag ggaagctaga cagtgacggt gatattcgtg tggaaggaat catacgcgga
    10561 tcaataagaa catcggcaaa ggttgcagtg ggttctaccg gacttatcga aggcgatatt
    10621 cactgtaaaa gcgctgatat cgaagggcgc atcatcggtg atgtggaggt tacggaagtg
    10681 cttacactga aatcaactgc gattgtagaa gggaatattt atactgcaaa aattgtgatt
    10741 gaaaacggcg ctcattttga cggcatatgt aatatgggca cgaaagaaaa ggaacgcaga
    10801 ataacacatg cccaacccga gctcgaagaa gccgcaattt gatagctaca tgaaatattc
    10861 cggaatggca tttcagctgt tcgcaggaat ttttttgggc gtttggggag gaataaaact
    10921 cgatgcgtgg ctgaacacaa aaccttggtt tactgttata ctttcattgt tcggaatcgc
    10981 agccgggatg tattctgtat tgaaagattt tattgattcc ggaaaaaaga agtagtagtt
    11041 catatgttga aatttgtagt ccgcttgata atctttgccg ttgttttggc aatcgtgttg
    11101 ttgattttag gaatggtatc cgaatttaaa cctgcaatga cctttgcatg gatgtgtttc
    11161 ggaatgtttt ttgcactcac tctgatcact tatttttttt ctgcaaaaac gatggagcga
    11221 aaattcagca gttttatgaa tgtcttcttt gtaggcattt tttcaaaact tattctttct
    11281 gccatcatgg tactcatttt taaagtgaag aatgaaacta caggattgaa ttatatcgtt
    11341 cctttcgcaa ttatctactt tacgttctta ttttttgaaa ccgtagagtt agtgaaattg
    11401 tcgagaagta ttggtaatcc gccacctcaa aaaccaacga aataatatgc gaattccggg
    11461 gttcttgctg ttgctctgtt cccccttttt tatcaaagct cagactcctg atctgcatgc
    11521 tttcgaaaag tcaatggttt ttgcagaaca acaagctgct gccggcaaaa tatcagttca
    11581 aagcacagcg cctacaaata attatgattt aatttatcaa aaatgtgcct ggaccataga
    11641 tcctgatact ttgtttattg atggcactat tacttcacaa cttgttttaa ccgaagaaag
    11701 taatgcgatt tattttgaca tttctgactc tttgaaggta gatgcagtaa ctgtaaatgg
    11761 ggtggaagga gattttctga ctgccaataa tgctgttcaa attaacttag atgatacgta
    11821 cgatataggt gctgaatttg aaattgcagt tacttatcat ggcacaccat caacaaatgg
    11881 gtttggctcc tttaagaaaa catcacaaga tgccggcaat gtaatatgga cactttcgga
    11941 gccttatggc gcaagtgatt ggtggccctg taaacaatca cttacagata aattggattc
    12001 agttgatgta tggattacca tgcccgataa ttgtaaatca ggtggtcccg gattgctcga
    12061 taccattaca gaagctggaa ttggatggca tacctaccgg tggaaacacc gatatccgat
    12121 tgctacttat ctgattggaa tatctgttac tgattttgat gaatttagtt tctatgctcc
    12181 gcatcatgaa gactcagtgc tgttttataa tcttgtgtat ccgtcaagtt ttgatgaagc
    12241 agttacacaa attaatggca tagtgcctgc ttttgaaatt ttttcagaca tctatggtga
    12301 ttacccattt gccgatgaaa aatatggtca catgcaattc ggatggggtg ggggaatgga
    12361 acatcaaacg atgagttctg tttcggggta tagtcatgaa ttattggtgc atgaaatggc
    12421 acatcaatgg tttggtgata aaataacctg cgcaagctgg catgatatat ggctgaatga
    12481 aggatttgct acttatagca cttggttgag ttatgatctt ggcgacgacc cgaataacta
    12541 ttacgaagca tggttgaaga ctacacgcga taaagtagta tcattacctg atggcagcgt
    12601 ttatgttgat gataccacgt tggtcagccg tatttttgat ggcagattaa cctattacaa
    12661 aggtggttgg gtgctccata tgttgcgttg ggaattgggt gatgatgctt tttttgcagg
    12721 cataaaaaat tatataaccg atcctgcatt ttcatttaag tttgttacta cgcaagatct
    12781 tatcagtcat ctcgaagaag ctgctgacac cagcctgacc gaatactttg atgattggtt
    12841 ttatggacaa ggatttccgg agtttcaaat aatctggcag caagatactt taggacattt
    12901 cattatgtct gtatctcaaa cgccaacaca tgaatcagtt gattttttca atatgcatgt
    12961 gcctttgcga ttgtatggaa atggtgattc acttgatttg cgactcaacc ataccgtaaa
    13021 tggtgaatta tttgaaatgg atcttccgtt catggttgat agtattgttt tagaccctga
    13081 attgcggata ttacatgcca atgacgccat tgttcacttg ccaataacag agggtatatt
    13141 gcaggcagtt atttatccca atcctgtatc tgatatcttg cacatgcaaa ttttagagcc
    13201 gtccgcttca acttataacc ttacaatttg gaatgctgct gctgaaaagg ttgtgtcagc
    13261 agaatttaat actagcggat cttcggcttc ctacacgatt gattcaacat cttggccatc
    13321 cggattatac atggtactga tcaaatccgg tgaaaaggag gtagtgcagt ctgttgtcgt
    13381 acaataatta aataccatac tttaatccga gcgtcagcct atctctattt atagatacaa
    13441 agtcatccgc tcctttggtc aatccggttg aataggtgag ctcaataaaa tatttattat
    13501 taatcgaaag gccggtctgt ccgacgatat gattggcaaa tttgcgataa ttaatactgt
    13561 tttcttcgtc tgttaccgtt acaacatcag gtacgtatat attgtatttt tcggtatgca
    13621 tactgtttga ctgttgaatt tcaaatgttg ctagatatcc ggctttaaga tatggtttga
    13681 aatgaggtgc attactttgc catttaaatt gtgttgacac caatgtctcc agtgctgaaa
    13741 aagtaaatga tcctgattca tgactattcc ctatttcacc gaaatatgtg ccagatgatt
    13801 tattttgtgc aattccacca tcaaaacata gccaaaagga ttcgcgatta ctggccatat
    13861 caatgtcata gaataatgca ccataatatg tcgggcccca attaaaatcg gcagtgccat
    13921 atccggttga aaccatggat attggatacg ttaccctgtt aatgccatac cctattgcaa
    13981 gtccgaatac tttctttaat ggcagtattg gtgaaatata gtcaataggc actgccagac
    14041 aagaatcaaa atttgtaatg gcttcgataa ggtcgtcggt tttaagatct gcatcgagag
    14101 aggctttggc tgctggggga cattcctcca tccattcata aagctgatcc ttgtaaacat
    14161 gcatgacaat gcctgattgt tcactttcac tataatattt tatgtcaatc aattcagtca
    14221 ttgaaccact atcatgtata aaataatgtt gtttccctct tgaatctttc aatgagtaca
    14281 agtccattcc tccatctgaa agcacaagtg tttttaaaaa gaccgtatcg ataatgattt
    14341 tctgagtaat cgtgcgaggc aattcgcgat aggtatatgg agtcccatcg agcccaatta
    14401 cagagctaat atagcattct cctgaaactg aaaatccagc aatagttagc gggttcatat
    14461 taatcgtcgg actttcctca ttctggcgaa aatgaatagt ttcaggcgat ttatcccatt
    14521 gtcgataatc aatataacct ggaattgtat cccccgtttt tgaaaatatt attgccggtt
    14581 gaaaattttg ttgcgcctga atgagaccat gtagacatgc tagcaaaagg attaaaacga
    14641 gtttcaagct gttcatgagg cattaataat tgtgtatatt cagtaaaaat ccgaaattgc
    14701 ctccaaataa ttcaatatat gaaaaaaatc atgctccttg gtagcggtga actgggaaag
    14761 gaattcgtta ttgcctgcaa gcgattaggc cattatgtaa ttgctgtaga tagttataac
    14821 gatgctcctg cacaacaggt tgctgatacg cgtgaagtca tttcaatgct tgatggtgat
    14881 gcgctggatg cgattgtgaa aaagcatgag cctgatatca tagttcccga gatcgaagcc
    14941 attcgcaccg agcgatttta tgattatgaa aagtggggta ttcaggtggt gcccagtgct
    15001 aaagcagcaa attttactat gaaccgtaag gccatccgtg atcttgcttc aaaagagttg
    15061 ggattgcgta cagcgaaata tgcctatgca ttgagtttcg aggaattaaa aactgctgtt
    15121 gcagaaattg gaattccatg tgttgtaaag ccactcatga gttccagtgg gaaaggtcag
    15181 agtgtaatta aaacagaagc agatatccaa acctcttggg attatgccca agccggaaaa
    15241 cgtggtgata ttgcagaagt gatcgtcgaa gaattcattc gttttgattc tgagatcaca
    15301 ttacttacag taactcaaaa aaacggccct accttatttt gcccgccgat aggtcaccga
    15361 caagaacgcg gcgattatca ggagagctgg cagcctgcac taataaaacc tgagcatctc
    15421 ctggaagccc aacatgtcgc taaacaggta accgaagccc tcaccggcgc cggaatatgg
    15481 ggtgtggaat tcttcctcgc agaaacaggc gtctattttt cagaattaag tcccaggccg
    15541 catgataccg gaatggtgac attagcagga acgcaaaatc tttcggaatt tgaattgcat
    15601 gcacgtgctg ttctcggtta tccggttccg gaaatcactt tggaaagggc aggagcaagt
    15661 gcagtaattc tggctgaaaa agaaagtaat agtttcagca ttgttggtgt agaaaaagcc
    15721 atgcaggaag tgaatacaga tgtcagaata ttcggtaaac caaccacacg caaataccgg
    15781 cgaatgggtg tggcgctggc ttgcggcaaa ccggatacaa atacagatgc attgcgaaaa
    15841 aaggctatga tcaatgcatc aaaggttgag attaaatact gacattacag ataaaaagga
    15901 agcgcgattc cggcagctaa ttcaaatcaa ttaagcatat ccaattcctt taattttgct
    15961 tttaattctt ctagtgagta ttctttaaat tcaacttcta ttttgtcatt gcttgttgtg
    16021 atatcctgaa acgccatgta tactttatcg ttcttatatt gaataattat a
//
LOCUS       DAOQOU010000057        16049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1837_length_16049_cov_4.820620, whole genome shotgun sequence.
ACCESSION   DAOQOU010000057 DAOQOU010000000
VERSION     DAOQOU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16049
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_16049_cov_4.820620"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLM90_06715"
     CDS             complement(<1..244)
                     /locus_tag="PLM90_06715"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ89099.1"
                     /translation="MTEHLSKEYWEQRYADGTTGWDMGVVAPALKQYFDSIERKDAAI
                     LIPGCGNAWEAKYLVEQGFTNITVIDIAEPPVNHLRE"
     gene            complement(241..885)
                     /locus_tag="PLM90_06720"
     CDS             complement(241..885)
                     /locus_tag="PLM90_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ89100.1"
                     /translation="MDDIRALLDNHFPQFEDALVDRIASVATLRTFARDEVMMYSGRY
                     FKSTILIVGGRVKLYRESNDGKEYFMYYIEPGNACALSMICAARNQASEITAVAVEDT
                     QVIQVPLEIMDDLMREYKSWYYFVLETYRSRFEELLVVIDQIAFKHMDERLEHYLFNQ
                     FNEYNSRRLEITHQQIANDLSSTREVISRLLKKMEQNGKIKLHRNEIELINLFV"
     gene            complement(914..1150)
                     /locus_tag="PLM90_06725"
     CDS             complement(914..1150)
                     /locus_tag="PLM90_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89101.1"
                     /translation="MWAKRFAAGWQYYLMASLTLGLAPFTPEPHIWGKIRWIAGGAVG
                     MQAIDWFDVFLHGTPWLLLIAALLIKAFRTGKQE"
     gene            complement(1140..1454)
                     /locus_tag="PLM90_06730"
     CDS             complement(1140..1454)
                     /locus_tag="PLM90_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ89102.1"
                     /translation="MIDLLKKMLGGGPKVDYAQLVKDGAVIIDVRTVGEFNGGHAKGA
                     INIPLDKLEGSIKKIKNKGPIIVCCASGMRSGSAKSILKSHGFTEVYNAGSWYNLRSH
                     VG"
     gene            1735..3402
                     /locus_tag="PLM90_06735"
     CDS             1735..3402
                     /locus_tag="PLM90_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89103.1"
                     /translation="MKTKFSFETYTLLASAFLGINRSGAQVVIKDYDPDLRMVKWDDT
                     FNLHDTVKIDIDENGINDLSFIYDYSGLDVIIEIEIADMVTGFGAEHDFSYDLWALDV
                     YAFHEGDIVDESVHFKDDGQNVASAWVTFTESGYYWNVYYRGNFNSYGYYLPLELNID
                     DSIHYGWARLSIGPLGNDLYNDMWYTTDYSLVVYEVGYEASPNTPIVCQTESPIEDIN
                     PILKNETDGDDFSEFTYFYHSAYFTDYAELRIFLIDNYNDAKNFSVAQAKLIDPANYE
                     VISGDTVPSYTDIYKHFDADTRTINGDPFDPNKYYVAYFMKIPLDGDTTHLTLSTPSK
                     LIKASKNACTLSLESSITKVNTTGTAADFDVTFSKDADESDIQEYRVGLISDPTEVFD
                     LANIVLDNYTLSVPITGASEYTLNLDGINYELNGSLIFFDQYYHAVIAAVSDGYLCDI
                     SCYVVTPNNAKTLIPNTAQSPGVTLTNYANLLTIQIDQPLQSEQAHLVLSSIAGEKVI
                     DTELVAGITDFNLNELPTGIYFATIYCKGQPIASKKIIVSLTTKGSH"
     gene            3801..5024
                     /locus_tag="PLM90_06740"
     CDS             3801..5024
                     /locus_tag="PLM90_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89104.1"
                     /translation="MKRGSLLIAIPFLGISAYGQTIDSAKVQSVLDKLELDVYGEIFY
                     NAYQWETFPKKNNDIDLSRLVLYPEYHFNDQIGVEMEIEFEHGGTGATMEYDYIEEYG
                     EFEMEIEKGGEVSIEELELNYTPTPWMEIIAGRIGLPIGLIAGDDKPLNYFTTTYNNV
                     EATLIPTAWYEFGAGAEFNFRNWEAKLVVVNALNSSLFSSANWIKPGNAMRFETTAAD
                     AFAVCLGINYNLNENSHIGVSTYRGNSTPNRPKEDISVDGNVSIADAHAKLQFGRWKI
                     NGLLIYGHLQNADLISEANRNLSNNLNAKRTPVASETMGYLGEIAFNILPKTNEHYRA
                     LDIFAGYYYYDSMYGTTGDIFNNPRWEKTEWRGGFCFTWNDNIAIKSDVTYRTLNIPD
                     VNTEITYTTALAFQF"
     gene            5035..6099
                     /locus_tag="PLM90_06745"
     CDS             5035..6099
                     /locus_tag="PLM90_06745"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HMZ89105.1"
                     /translation="MFNIQLKHLLTLPATAIIISSCNNNDDPSDIGTYDFSPILNHVS
                     ENVITATYGDLHSKSQSLITAVENFNADRSSTNLEAARQAWRDCRIPWEQSEGFLFGP
                     VSTEGIDPAIDSWPVNVIDLDAVLASGESLTKAYVDALEGTLKGFHTIEYLLWGTDGD
                     KSAAAFTDREFEYLSAVTQSLEGATQQLYDAWHSGGDNFQLNIAQAGTATSIYPSQKS
                     AMQELVNGMIGIADEVANGKINEPLTNMDVTLEESHFSSNSKADFMDNIRSIKNVYLG
                     SYLMDGEGISDYIKSLNSSVDDDVIAHIDAAILAIENIPGDFSDAIFMNTASVEAAQQ
                     SVRDLQSILESAVKPLIDNY"
     gene            6124..7491
                     /locus_tag="PLM90_06750"
     CDS             6124..7491
                     /locus_tag="PLM90_06750"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HMZ89106.1"
                     /translation="MRRSLVVMSIITSGIIFLTIHACSIDPQVYGEWYNEEEYYAGGE
                     TTIFEATSGAFSTPAPNLSPLQLEKHLSGDMEFEATFVTAPSPVNAGLGPVFNQTSCV
                     GCHTLDGRGDKPTVFRISTPGTDAHGGPLGVAGFGGAIQDKAIFDVAPEAMVDISYVE
                     STYSFPDGETYSLRKPIYTIINNYAPLPSDVALSVRVAPPVFGLGLLEIIPEEAIIAL
                     ADEQDADKNGISGKANYVYDVRTGLMALGRFGWKANQPNLDQQNAAAFNEDMGITNPI
                     FQTESCFGQLQFDGITDEPEITMPVLEDVTFYVRTLAVPAPRNLEDANIQEGKMYFYK
                     AGCNTCHNPSFTTGIAINAPELSNQKIYPYTDMLLHDMGDAMGDGRQDYLATGNEWKT
                     RPLWGIGLTQLANGHTVFLHDGRARNLTEAIMWHGGEAEASTEYFASLTKEQRDKLLE
                     FLMAL"
     assembly_gap    7933..8032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8309..8677)
                     /gene="ssrA"
                     /locus_tag="PLM90_06755"
     tmRNA           complement(8309..8677)
                     /gene="ssrA"
                     /locus_tag="PLM90_06755"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            8748..10442
                     /locus_tag="PLM90_06760"
     CDS             8748..10442
                     /locus_tag="PLM90_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ89107.1"
                     /translation="MASDNTNNATQRPYPRIIPDIDNWPIYTLSKDREQLIESVIDET
                     LENILKKNPTEKDIHDELAKAVYLEKIRVERPWKTDAPDEKTFWNGVKNKLVQLESHP
                     DNKIDEEKAILRTIIHRYVHEIVGNFNKGTYNFAERFVPFAFNRLLNASHSRAFTRLF
                     GNKRDLLKSIHLIGEIEQVRELAKDGIIVAVPTHSSNIDSITVGWGISAIGLPAMFYG
                     AGLNLFSVRFLAYFMNRLGAYRVDRRKKNAVYLEALKTYSTQVITKGGHSLFFPGGTR
                     SRSNVVESQLKLGLLGTALEAQRRLILETNGQSFKKVYVVPVTMNYGFVLEAKELIDE
                     HLKNVGKERYFVPSDKSSSSFKILKFMYRFFTADADFTIAFGKCMDIFGNPVDKQGNS
                     LDHNGNMIDIRDYFRSHGQLTNDTQRDSVYTKMLSEAIVVAFKKNNVALPEHVIAFAA
                     FKLFQKKHHTSDLYSVLRMAEEFRELTREELKQAVASIVKELSKLEHKEEIRVDPIVA
                     AEDLDLLIDYAIKKLGVYHQKRPLLKTRKGTIKCQDMKLLLFYHNRLEGYGLEKHV"
     gene            10435..11478
                     /locus_tag="PLM90_06765"
     CDS             10435..11478
                     /locus_tag="PLM90_06765"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HMZ89108.1"
                     /translation="MSDKTSVGVVGSGSFGTAVANLLAENSHVFLYSRRADNVVSMAN
                     ARISSGQQLHPNITPTDDIGLIAKECYVIFPIVSSDGFKEVIRTMAPFLRPDHILIHG
                     TKGLALKKKGLEKLKEKSRISNRNVLTMSQLIRRESVVVRVGSMAGPNLAREIAQEMP
                     AATVIASRFDEVIHEGHRLLTSPRFQVYGSYDIIGVELAGVLKNILAIGSGIVSGLNL
                     GENARALLITKGWAEIIKLSEVLGADVKSFLGLAGIGDIIATCSSPSSRNFSVGFRLA
                     KGEKIKAIQTSMEEVAEGINTIRIANGLANFHGIHCPIIKTLYQGIFEDLNVQTGIEY
                     LMRSRRSMDVEFL"
     gene            complement(11482..11985)
                     /locus_tag="PLM90_06770"
     CDS             complement(11482..11985)
                     /locus_tag="PLM90_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HMZ89109.1"
                     /translation="MRKLSVVLGFVALSLSVVGCKNDESEPLVKPHHLGAGQPQFQKQ
                     GELTFLNPEGAALSKINIEIADTPDRQQKGLMNRSFMSNDNGMLFIFDVERPQSFWMR
                     NTIIPLDIIYVNSKMQIVSIAKNTTPYSEESIPSRGPAIYVVEVNGGYCDQYSIQPGC
                     LIQFTKL"
     gene            12145..12933
                     /locus_tag="PLM90_06775"
     CDS             12145..12933
                     /locus_tag="PLM90_06775"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HMZ89110.1"
                     /translation="MQIVDNPDQKTPPASEPKEPVRQKRFYLTGTALLVLGIIYFLQK
                     ATNLETPLDFISWELILIITGVYIGEKHNFTGVSWLICIAIGAWFLLDSYIPTVNLRY
                     YFGPIILIAIGAYLLFGDRRIRYRRNKYRNYGRPYTKEQGFAADNSASDDYIDVVSVF
                     GGVSKQMFTKQFKGGEATSIFGGTEINLMQADFEGKAVLELTQIFGGCTLIIPAHWDL
                     KTEVVSIFGGVEDNRRGNTTLIDHKRVLLLKGTSIFGGIEIKSY"
     gene            12945..13997
                     /locus_tag="PLM90_06780"
     CDS             12945..13997
                     /locus_tag="PLM90_06780"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ89111.1"
                     /translation="MSTKRIPVNKYATPFLLSWFIWSCVYAGLLKYLGANLSIAVIDS
                     LVFNGALLLCAILQVVVLQYYFPKQNKTSYVLVVSLIVSTIVTAFSTFIMSQFPEQQF
                     SITTQQDVYISYTNIAIPLRFSLAFLLAACIAMINILFFTLQEEKSTVQRKIEAEQLA
                     REAELFQLRSQLQPHFLFNSLNSISALAGSKPEEARRMIQQLSDFLRGTIKKDNSQLI
                     TLEEEIKHLSLYLDIEKVRFGHRLQTNIDCPDDCYKLKIPSLLLQPVLENAIKFGLYD
                     TLEEVSIDIRARYDEPYLKVEITNPFDKDTQPAKGTGFGLQAVSRRLYLLYARTDLLQ
                     TSSEQHIFKTVITIPQ"
     gene            13994..14734
                     /locus_tag="PLM90_06785"
     CDS             13994..14734
                     /locus_tag="PLM90_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HMZ89112.1"
                     /translation="MNPIKVIIIDDEPLARSIVAEYIASHSQLTIVAECGDGFEGVKA
                     IAQHQPDLIFLDVQMPKINGFEMLELLEDKPDVIFTTAFDEYAIKAFDIHAIDYLLKP
                     FNKERFDAAISRWQSMRGTKTATTLSTEEIIPAEQQPQRVVIKLNNKIKIIPFSDIHF
                     IEAAEDYVMIHTGEGRYMKHKTMTFFEKALPANQFVRCHRSYIVDVQQINRLEAYEKD
                     SQIAILKSGAKVPVSKSGYIRLKQVLGV"
     gene            complement(15173..>16049)
                     /gene="groEL"
                     /locus_tag="PLM90_06790"
     CDS             complement(15173..>16049)
                     /gene="groEL"
                     /locus_tag="PLM90_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMZ89113.1"
                     /translation="DVDGEALSTLVVNKIRGTIKVCAVKAPGFGDRRKEMLQDIAILT
                     GGTVISEEQGYKLENADLTYLGRAERVTIDKDNTTIVGGKGKKDDIKARINEIKAQIE
                     KTTSDYDREKLQERLAKLSGGVAVLYVGAATEVAMKEKKDRVDDALHATRAAVEEGIV
                     AGGGVAYIRAIEALDKIRGGNEDEKIGIEIIKRSLEAPIRTIVGNAGIEGSIVVQKVR
                     EGKADFGFNARTEKYENLLKAGVIDPVKVTRVALENAASIAGLLLTTECTIVDKPEKK
                     EPMGGGMPGGMDGMM"
BASE COUNT         4766 a         3347 c         3333 g         4503 t
ORIGIN      
        1 cttcgcgtaa atgatttaca ggaggttcgg caatatcgat aacggtgata ttggtaaatc
       61 cttgttctac aagatatttt gcttcccagg cattaccgca tccgggaatt aatatggctg
      121 catcttttct ttcaatgcta tcgaaatact gttttaatgc cggagcaacc acgcccatat
      181 cccaacctgt tgtgccatca gcataacgct gctcccaata ctcttttgat aagtgctctg
      241 tcatacaaat aaattaatga gctctatttc gttgcgatgc aattttatct tgccgttttg
      301 ttccattttt ttcagcaatc ttgaaatcac ctcacgcgtg gaactcaggt cgttcgctat
      361 ttgctgatgt gttatttcaa gacggcgaga attatattca ttgaactgat tgaataaata
      421 atgttccaat cgttcatcca tatgtttgaa cgcaatctga tcaataacaa ccagtaattc
      481 ttcaaaacgt gaacggtaag tctccaatac aaagtagtac cacgatttat attcacgcat
      541 caagtcatcc atgatctcta gcggaacctg aataacctgt gtatcttcaa cggctacagc
      601 agtaatctca cttgcctgat tgcgggcagc acatatcatc gacaaggcgc aagcgtttcc
      661 gggttcgata taatacatga aatactcctt accatcattg ctttcgcgat agagttttac
      721 gcgtccccca acaatgagaa tggtcgattt aaaatatcgc ccggaataca tcattacctc
      781 gtcgcgggca aatgtgcgca acgtagctac cgaggcgatg cggtcaacaa gggcatcttc
      841 aaattgcgga aaatgattgt ccagtagagc tctgatatca tccatacaac agcaaatcta
      901 caacaaggaa caattattcc tgtttaccgg ttcggaatgc ttttatgagt agagcagcaa
      961 tcaacaaaag ccagggcgta ccgtgcagaa acacatcaaa ccagtcaatc gcctgcatac
     1021 caacagcacc tcctgcaatc caacgtattt taccccagat atgcggctcg ggagtgaatg
     1081 gagcgagtcc taaggttaaa gatgccatca ggtaatattg ccaaccggca gcaaaacgct
     1141 tagcccacat gcgaacggag attataccaa cttccggcat tgtaaacctc agtaaagcca
     1201 tgcgatttca gtatcgattt tgccgaacca ctgcgcatgc ctgaagcaca acatacaata
     1261 atgggacctt tgtttttaat ttttttgatg cttccctcca gtttatccag cgggatattg
     1321 atagcgcctt tcgcgtgacc accattaaat tcaccaacag tgcgcacatc aataatcacc
     1381 gctccatcct tcaccagttg tgcataatcc accttcgggc caccacctaa catttttttg
     1441 agtaagtcta tcatcctgta atttttgccc aaaggtagag ccgcaaattg ccctcgtcag
     1501 tgacaaatat tactcgtgaa tcgtgaatcg tgaatcgtga gtggtcagtg gtgggtcgtg
     1561 agcttgccga taggcggagt tgcccattga ccaatgtgaa ctgtgattcc gggtaaggta
     1621 tcaatggaat aatgttatta atcaaacaat ccttaacttt atttaaaata tacgtgaatt
     1681 tgaggcatcc caccctcgaa tcaaccgtga agctatatca gacacgttaa acctatgaaa
     1741 acaaaattct ccttcgaaac ttatacactg ttggcatctg catttcttgg gatcaataga
     1801 tcgggtgctc aagtggtcat caaagactac gaccctgatc ttcgcatggt taaatgggat
     1861 gacactttca atttacatga tacagttaaa attgatatcg acgaaaatgg cattaacgac
     1921 ttgagtttta tatatgatta cagtggctta gatgtaataa tagagattga aattgccgac
     1981 atggttaccg gttttggagc ggaacacgat ttttcttacg acctgtgggc acttgatgta
     2041 tatgcatttc acgaaggcga tattgttgat gaatctgtcc attttaaaga tgacggacaa
     2101 aacgtagcat cagcatgggt aacatttaca gaatccggat attattggaa tgtgtattat
     2161 cgtggaaatt tcaattcata tggatattat ttgccattag aattgaatat tgacgactcc
     2221 atacattacg gatgggcgag attgtcaata gggccattag gaaatgatct gtataatgat
     2281 atgtggtata ctacagatta ttcacttgta gtgtatgagg ttggatatga agcatcgcca
     2341 aatacaccta tcgtttgtca aactgaatct ccaattgaag acattaaccc gattttaaaa
     2401 aatgagactg atggagatga cttttcagag ttcacatatt tttatcactc agcctatttt
     2461 acagattatg ccgaactcag aatattttta attgacaatt acaacgacgc aaaaaacttt
     2521 tcagtcgccc aagccaaatt gatcgatcca gcaaattatg aagtgatttc tggcgacacc
     2581 gtgccttcat ataccgacat atacaagcac tttgatgccg atacaagaac cataaatggt
     2641 gacccattcg acccgaacaa atactacgtt gcctatttca tgaaaattcc tctggatgga
     2701 gatactaccc atttaacact gtcaacacct agtaagctaa tcaaagcttc taagaatgcg
     2761 tgcactttgt cgcttgaatc cagtatcacc aaagtgaata caacaggcac agccgcagat
     2821 tttgatgtta ctttctcgaa agatgctgat gagagcgaca tccaagaata ccgagtagga
     2881 ttgatcagcg accctaccga agtttttgat ctcgcaaata tcgtattaga taactatacc
     2941 ttaagtgtgc cgatcaccgg tgcatcagaa tacacgctta atctggatgg aataaactat
     3001 gaactgaatg gaagtttgat cttctttgat cagtattacc atgctgtaat agctgctgtt
     3061 agtgatggat atttatgtga tatttcatgc tatgtggtaa cgccgaataa tgccaaaacg
     3121 ctcataccca acacagctca atcgcctggc gtcactttga ccaattacgc gaacttgcta
     3181 accattcaaa tcgatcaacc gttgcaatca gaacaagcac atttggtatt atcgtcaatt
     3241 gcaggagaaa aggtaattga taccgaattg gttgcaggta taactgactt caacttaaac
     3301 gaacttccaa cgggaatcta ttttgcaact atatattgca agggtcaacc cattgcgtca
     3361 aagaaaataa tcgtaagcct tacgactaaa ggtagccatt gaggccgtca tcatctcact
     3421 tcgcccaaat caaaccctgc gatcttcgcg tcctccgcgc ttaacccgca aattcatata
     3481 gaatcatata tcccgatttt tgaggtagcc cggaaaaaca gttactcata attaaacccg
     3541 gcgccgcaaa ccaacatttt tcctgaattc agtgatttgt agaattcatt atcaattgaa
     3601 catactgatt gccaaatact tatgattaga gattttgtga ttaagcgctg aggacaccgg
     3661 gggcgcatcg catgttgggt caactttctg gagttccagt taggtgatac cacaatatag
     3721 gtattattta gattttgtct aaataatatt ggaaactgcc ccgccggagg atatttttgc
     3781 cgccaacagg cgaaaattca atgaaaagag gctcattatt gattgcgata ccctttctgg
     3841 gcatttctgc ttacggacaa actattgatt ctgcaaaagt ccaatcggtg ctggataagc
     3901 tggaattgga tgtatatggt gaaatattct acaatgctta tcaatgggaa accttcccga
     3961 aaaagaacaa cgatatagac ctgagcaggt tggtgcttta tccggaatat catttcaatg
     4021 atcaaattgg tgtggagatg gaaatcgagt tcgagcacgg aggaacaggt gccaccatgg
     4081 aatatgatta catagaggag tatggcgaat ttgagatgga aatagaaaaa ggcggcgaag
     4141 tatcgataga agagttggaa ttgaattaca cccctacccc atggatggaa attattgccg
     4201 gaagaatagg tttgccgatt ggattaattg ccggcgacga taaacctctg aattatttca
     4261 caaccaccta caacaatgtt gaggcgactt taataccaac ggcctggtat gaatttggtg
     4321 caggggcaga atttaatttc cggaattggg aagcaaagct tgttgtggtg aatgccctga
     4381 acagctcact gtttagttct gccaactgga taaaaccggg taacgctatg cgcttcgaga
     4441 ctactgctgc agatgcattt gccgtatgtc tgggaattaa ttacaatctc aatgaaaaca
     4501 gtcacatcgg tgtaagtact tatcgtggca atagtacacc caatcgtccg aaggaagata
     4561 tatcagttga tggcaatgtt tcgattgcag atgcacatgc gaagttgcaa ttcggaagat
     4621 ggaaaataaa tggtttgctg atatacggtc atcttcaaaa tgcagatctc atcagcgaag
     4681 caaacaggaa tctgagcaac aacctcaacg ccaaacgcac ccctgttgca agtgaaacca
     4741 tgggttacct tggcgaaatt gccttcaaca tactgccaaa aacaaatgaa cattatagag
     4801 ccctcgacat ttttgccgga tattattatt atgactcgat gtatggcact accggcgaca
     4861 tattcaataa tccaagatgg gagaaaacag aatggcgcgg gggattctgt tttacatgga
     4921 atgataatat cgccatcaaa tcagatgtca cctatcgcac attgaatatt cccgatgtaa
     4981 atacagaaat cacttacaca acagctcttg catttcaatt ttaaaataaa cactatgttc
     5041 aatatacaac taaaacacct cctcacactt ccggcaactg caataataat atcgtcatgc
     5101 aacaataatg acgatccttc agatattggc acctatgatt tttcaccgat attaaatcac
     5161 gtgtcggaaa atgtaattac cgctacttat ggcgacttac acagcaaatc gcaatcattg
     5221 atcacagcag ttgaaaattt taatgccgac agatcgtcaa ccaatttaga agcggcacgt
     5281 caggcatggc gcgattgcag aattccatgg gaacagagtg aaggattttt atttggccct
     5341 gtttctacag aaggaataga tcctgctatt gacagttggc ctgtaaatgt tattgatctt
     5401 gatgcagtac tggcgagtgg cgaatcactt acaaaggcat atgtagatgc acttgaaggt
     5461 acattaaaag gttttcacac cattgaatat ttattgtggg gtacagacgg cgataaatcg
     5521 gcagcagcat ttaccgatag agaatttgaa tatttaagtg ccgtaactca aagtctggaa
     5581 ggcgctaccc aacaattata cgatgcctgg cacagtggtg gcgataattt tcaattaaac
     5641 attgcgcaag caggcactgc aaccagtatt tatcccagtc aaaaatctgc tatgcaagaa
     5701 ttagtaaatg gcatgatcgg catagcagat gaggttgcca acggaaaaat caacgagccg
     5761 ctcacgaata tggatgtcac cttggaagaa agtcatttca gttctaacag caaggctgat
     5821 tttatggata atatccgcag tataaaaaat gtgtatctgg gtagctacct catggatggt
     5881 gaaggcatca gcgattatat caaatcatta aattcatcgg tagatgacga tgtgattgcg
     5941 catatcgacg cagccattct tgccatagaa aatattcccg gcgatttttc agatgccatc
     6001 ttcatgaata cagcatcagt agaagcagcg caacaaagtg ttcgtgattt gcaaagcata
     6061 ttggaatctg ccgttaagcc attgatcgat aattattaat cacagatgaa aaccattaac
     6121 ccgatgcgtc gatcacttgt ggtgatgagt attattacct caggcattat tttcctcact
     6181 attcatgcat gctccatcga cccacaagta tatggcgaat ggtataatga agaagaatat
     6241 tatgccggtg gtgagacaac tatattcgaa gctaccagtg gcgctttctc tactcctgct
     6301 ccgaatttgt caccattgca actcgaaaaa catctttcgg gcgatatgga atttgaggca
     6361 acatttgtga cggcaccttc accggtaaat gcgggacttg gacctgtatt taatcagaca
     6421 tcttgcgtag gatgtcatac tttagatggc agaggagata aacctactgt ttttagaata
     6481 agcactccgg gaacagatgc acatggcgga ccacttggtg ttgcaggatt tggcggagcg
     6541 atacaggaca aagccatatt tgacgttgca ccggaggcaa tggttgatat cagttatgtt
     6601 gaatcaacat atagttttcc cgatggcgaa acttacagtc tgcgaaaacc gatttataca
     6661 ataatcaata attatgctcc gcttccgtct gatgttgctt tatcggtgcg tgtggcgcca
     6721 cctgtattcg gacttggatt gcttgaaata attcctgaag aggcaattat cgctcttgcc
     6781 gatgaacaag atgccgataa aaatggcatt tccggaaagg ctaattatgt atatgatgtg
     6841 cgcaccggat tgatggcttt ggggcgattt ggatggaaag caaatcagcc aaatttagat
     6901 cagcaaaatg cagcagcttt caatgaagat atgggaataa ccaatccaat ttttcaaact
     6961 gaatcctgtt ttggacaatt acaattcgat ggcataaccg atgaacctga aatcacgatg
     7021 ccggttttgg aggatgttac tttttatgtg cgcacacttg ccgttcctgc accgagaaat
     7081 ctcgaagatg caaatattca ggaagggaaa atgtatttct ataaagccgg ttgcaatacc
     7141 tgtcacaatc catcttttac aacaggcatt gctattaatg cgcccgagct ttccaatcaa
     7201 aaaatctatc cgtataccga tatgttgttg catgatatgg gcgatgcgat gggtgatggc
     7261 cgacaggatt atttagccac aggaaatgaa tggaaaacgc ggccgctttg gggaatagga
     7321 ttaacacaac ttgcaaatgg tcacaccgtt tttcttcacg acggacgtgc acgcaatctc
     7381 accgaagcta ttatgtggca tggtggcgaa gcggaggcat ctacggaata tttcgcatca
     7441 ctgactaagg agcagcgtga taagttgctg gaatttttga tggcattgtg atgcgtgaat
     7501 cgtgagtcgt gagtcgtgaa tcgtgagatt gaattttctt tgcgactccg catctttgcg
     7561 actttgcgcg aaataacact gcaaatttta tggtattaaa aatctacttt ccataactat
     7621 aattcacatt caaataaaaa agcctgcttc atatttatat gtctattatc atgtgtgatg
     7681 tatgcagaat gttttggttt gatgatttga atattatttg aagatggcga gaaggaaaca
     7741 ccgttgaatt acaatcctct gtttaagcgc ggaggacgcg gaggtgcgca aagaaaaatg
     7801 cagaatgcgt tagtttgatg atttgaatat tatttgaaga tggcgagaag gaaacaccgt
     7861 tgaattacaa ccctctgttt aagcgcggag gacgcggagg tgcgcaaagg aaaatgcaga
     7921 atgcgttggt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgcacact
     8041 tccacacttg cacacttgca tacctgcaca cttccacact tgcacacttc cacacttgca
     8101 cacttccaca cttgcacact tccacacttt ttcagtggag ttggtgggat tatttgcatg
     8161 atcccttcgt ggggaggcta caataaaaaa accaaaaccg cttcgctata aatgcaaatg
     8221 ccaattttca tcgcttgtaa aatttacaga ctctgaaaat cggcatttgc atttactttt
     8281 gctgaggcac aaggttttgg tttttttatt gtggagttgg tgggattcga acccacgtcc
     8341 agacaaggca ttagcaagct ttctacatgc ttagtgcgct cttgattgtc gggttatgca
     8401 aggtgagaac acttacctga gtataacctt agcttcttga tctcatcctg catcgaagca
     8461 ctgcaggacc agtccggtga gatgacgttc gtcggaaccg taccagacaa acggttaccg
     8521 ggaacgaagg ctactctaat tctctgaatt aagcggccaa ggcgtagtta gactcgccat
     8581 gtaaaaaaag ggttgtagct tgagattaaa gagccaaatt acaatgctct gcatgcttac
     8641 ctgtcaatga acgatgctgt caattccggt caaccccgaa tgatcattaa accgcaaaac
     8701 tacatggaaa agttcatttt gcctcccaat cggctatatt tgcagaaatg gcttcagaca
     8761 acacgaataa tgccacccag cgtccatatc cgagaatcat tccggatatt gacaattggc
     8821 ctatatatac cctcagcaag gaccgtgaac agttgataga atcggtgatc gacgaaacac
     8881 tcgaaaatat cctcaagaag aatcctaccg agaaagacat acacgacgag ctagccaaag
     8941 ccgtttacct cgaaaaaata cgtgtagaac gcccctggaa aaccgatgcc ccggatgaaa
     9001 aaactttctg gaatggggtt aagaacaaat tggttcaact agaaagtcac cccgacaata
     9061 aaatcgatga ggaaaaggca attttgcgca ccatcattca tcgttatgtg cacgagatag
     9121 tcgggaactt caacaaaggc acttacaatt ttgccgagcg atttgtgcct ttcgccttca
     9181 accgattact caatgcctcc catagcagag cttttaccag attgtttggc aacaaacgcg
     9241 atttactgaa aagcattcat cttatcggtg aaattgaaca agtgcgcgag cttgctaaag
     9301 atggaattat tgttgccgtt cctacacata gcagcaatat cgattcaata acagttggat
     9361 ggggaatttc tgccatcgga ctgccggcca tgttttatgg tgcgggatta aatctgttct
     9421 cagtgcgatt tcttgcctat tttatgaata gactcggtgc ttatcgcgtc gaccgcagga
     9481 agaaaaatgc tgtatatctt gaagccctaa aaacttattc cacacaagta attaccaagg
     9541 gcggacatag tttatttttc ccgggtggaa cgcgatcgcg cagcaatgtt gttgaatcgc
     9601 aattaaaact cggattgctg ggaacagcgc tggaagccca acgccgcctc attcttgaaa
     9661 caaatggcca gtcctttaaa aaggtgtacg ttgttcctgt aaccatgaat tatggctttg
     9721 ttttggaagc taaggaattg atagatgaac atctgaaaaa tgttggcaaa gaacgatatt
     9781 tcgtgccttc ggataaatcg agttcctctt tcaaaattct aaaattcatg taccgctttt
     9841 ttactgccga tgctgatttt accatcgcct tcggcaagtg tatggatata tttggaaatc
     9901 ctgtcgataa gcaaggtaat agtctcgatc acaatggcaa catgatagac attcgcgatt
     9961 atttccgctc gcatggacag cttaccaatg atacacagcg cgattctgtg tatacgaaaa
    10021 tgttatccga agcaattgtt gttgcattca aaaagaataa tgttgccttg cctgaacatg
    10081 tcatcgcatt tgcggcattt aaattatttc agaaaaaaca tcataccagc gatctctatt
    10141 ccgtattgcg catggctgaa gaattcaggg aacttactcg cgaagaattg aagcaagctg
    10201 ttgcatccat tgtcaaggaa ttaagtaaac tggaacacaa agaagaaata cgtgtagatc
    10261 cgattgttgc tgcagaagat ctcgatttat tgattgatta tgccatcaaa aaactgggtg
    10321 tatatcatca gaaacgacca cttctgaaaa cacgcaaagg cactatcaaa tgccaggata
    10381 tgaaattgtt gctattttat cataatcgcc tcgaaggata cggattagaa aaacatgtct
    10441 gacaaaacca gcgttggagt tgtaggttcc ggaagcttcg gaacggcggt agccaatctt
    10501 ctggcggaaa acagtcatgt gtttctgtat tcacgtcgtg ccgacaatgt ggtatccatg
    10561 gcaaatgcac gcatatcgtc aggtcaacaa ttacatccca atatcacccc caccgacgat
    10621 attggtctga ttgcaaaaga atgctatgta atatttccca ttgtttccag cgatggtttt
    10681 aaggaagtga tcagaaccat ggctcctttt ttgcgtcctg atcatatttt aattcatggc
    10741 accaagggtt tagcattaaa gaaaaaagga cttgaaaaac tgaaagaaaa atcgcgcata
    10801 tccaatcgca atgtgcttac catgagtcag ctcattcgca gagaaagtgt tgtggtgcgt
    10861 gtcggcagta tggctggtcc aaatctggca agagagattg cacaagaaat gcccgcggct
    10921 actgttattg caagcagatt tgatgaggtt attcacgaag gacatcgttt gcttaccagt
    10981 ccgaggtttc aggtttatgg ttcttatgat attattggcg ttgaattagc cggcgtttta
    11041 aaaaatattc tggctatcgg cagtggtatt gtgagtggac tcaatctcgg cgaaaatgca
    11101 cgtgctctgc tcattacaaa aggctgggcc gaaatcatta agctgagtga agtattgggt
    11161 gccgatgtaa aatcattcct cggacttgcg ggaatcggtg atattattgc tacttgctca
    11221 tcaccaagca gtcgaaattt cagcgttgga tttcgtttgg caaaaggcga aaaaataaaa
    11281 gctattcaaa ccagcatgga agaggttgca gaaggtatta ataccatacg cattgccaat
    11341 ggcctcgcaa atttccatgg catacactgc cctatcataa aaacattata ccagggaatt
    11401 tttgaagacc tcaatgtaca aacaggcata gaatacctga tgcgcagcag aagaagtatg
    11461 gatgtggaat ttctttaatt ctcagagctt ggtaaactga atcaggcatc cgggttgaat
    11521 actatattga tcgcaataac caccattcac ctccaccaca taaattgcag gtccgcgact
    11581 tggaattgat tcctcagaat acggtgtcgt attttttgcg atggacacaa tttgcatttt
    11641 gctgtttaca taaataatat ccaaagggat gatcgtattt ctcatccaaa atgactgtgg
    11701 acgttcaaca tcaaaaatga acagcatgcc attatcgttc gacataaaag atctgttcat
    11761 tagtcctttt tgttgacggt caggggtatc tgcaatttcg atattgattt tagataatgc
    11821 agcaccttca ggattcaaaa atgtgagctc accctgcttt tgaaattgag gctgtccggc
    11881 tccaagatga tgcggtttta ccagaggttc agattcatcg ttcttacacc caacaacact
    11941 taatgacaag gcaacaaagc ccaaaacaac ggatagttta cgcataaacg gtaattacgg
    12001 agcaaagata gacgaatttc catgttttgc tccccttcat tcgccgccaa aattgtgcca
    12061 ttggtcgaac ttttggctca ccagatgcct ttgtgcttgc tagaaagctt cagacgggga
    12121 tacatttgct taaaattgat tgatatgcag atcgttgaca atcccgacca gaagacccct
    12181 cctgcttccg aaccaaaaga acccgttcgc caaaaaaggt tttacctcac aggaacggca
    12241 ttgctggttc taggtattat ttacttcctc caaaaagcga caaaccttga aacaccactt
    12301 gatttcatca gctgggaatt gatattgatc atcaccgggg tttacattgg cgagaaacac
    12361 aatttcacag gtgtgtcatg gttgatttgc atagccattg gtgcttggtt tttgctcgat
    12421 tcctatatac caacagtaaa tctacgatat tactttgggc ccatcattct aattgccatc
    12481 ggtgcgtatt tattattcgg cgatagaaga attcgctacc gtcgcaataa atatcgcaat
    12541 tacggtcgcc cctacacgaa ggaacaagga ttcgcagctg acaacagtgc ctctgatgat
    12601 tatattgatg ttgtttcagt attcggcggt gtaagcaaac agatgtttac caaacaattc
    12661 aaaggtggcg aagcgacaag tattttcggc ggcacagaaa ttaatttgat gcaggccgat
    12721 ttcgaaggga aggcagtatt ggaactcact caaattttcg gcggatgcac actgataatc
    12781 cctgctcact gggatttaaa aactgaggtg gtttctatat tcggtggtgt tgaagacaat
    12841 cgtcgcggca acactacgct tatagatcat aagcgcgtat tattattgaa aggaacatct
    12901 atttttggcg ggatagagat taaaagttac tgatactaac tttcatgtcg acaaaacgca
    12961 tacctgtaaa caagtacgca actccttttt tattgagttg gtttatttgg tcatgcgttt
    13021 atgcgggttt gttaaaatat ctgggcgcaa atctttcaat agccgtcatc gacagcttag
    13081 tatttaatgg tgccttattg ttatgtgcaa ttctacaagt ggtagtgctg cagtattact
    13141 tcccgaagca aaataaaacg agttatgtat tagttgtttc acttattgtc agcacaattg
    13201 taactgcatt cagcacattt attatgtcgc agtttcctga acagcagttt tcgataacaa
    13261 cgcaacaaga tgtatatatc agctatacca atatcgccat tccgctgcga ttcagtctgg
    13321 catttttatt ggctgcctgt attgccatga ttaatatttt gttctttacg ctacaagaag
    13381 aaaaatcgac tgtgcaaaga aaaattgaag ctgaacagct tgcccgtgag gcggaattat
    13441 tccaattgcg cagtcagttg caaccgcatt tcttatttaa cagcttgaat tccatcagtg
    13501 cattagcagg cagcaaacct gaagaagctc gacgtatgat acaacagcta tcagattttt
    13561 tgcgaggcac aataaaaaaa gataacagtc agctcatcac acttgaagaa gaaataaaac
    13621 acctttcact atatctcgat atcgagaaag tgcggttcgg acatcgtttg cagacgaata
    13681 ttgattgccc tgatgattgt tataaattaa aaataccatc cttattattg caacccgttc
    13741 ttgaaaatgc aataaaattc ggattgtatg atacgcttga agaagtaagt atcgacatac
    13801 gagcaagata tgatgagcca tatcttaagg tggagattac caatcctttt gataaggaca
    13861 cacagcctgc aaaagggacg gggtttggtt tacaggctgt gagcagaaga ttatatttgc
    13921 tgtatgccag gacagactta ctccaaacaa gctctgagca acatattttt aaaactgtaa
    13981 ttaccattcc gcaatgaatc cgataaaagt cattattatc gacgacgagc ccttagcacg
    14041 cagtatcgta gcagaatata ttgcttcgca tagtcagtta accatagttg cagaatgtgg
    14101 cgatggattt gagggtgtga aggcaattgc tcagcatcaa ccggatttga ttttccttga
    14161 tgtgcagatg ccaaaaataa atggatttga aatgctcgag cttctggagg acaaaccgga
    14221 tgttattttc acaacggcat ttgatgaata tgcgataaaa gcatttgata ttcatgccat
    14281 cgattatttg ctcaagccat ttaataaaga gcgatttgat gctgcaatat cgagatggca
    14341 gtcgatgcgt ggaacgaaaa cagcaactac tctttcaacg gaagaaataa ttcccgcaga
    14401 gcaacagcct cagcgagttg ttatcaagct caacaataaa atcaagatca taccattttc
    14461 cgacattcat tttattgaag ctgctgagga ctatgtgatg atacataccg gtgaaggccg
    14521 gtatatgaag cataaaacaa tgaccttttt cgaaaaggca ttgcctgcaa atcagtttgt
    14581 gcgctgtcat aggagttata ttgttgatgt gcaacaaatc aatcgactgg aggcctatga
    14641 aaaggattca caaatcgcga ttctaaaatc cggcgctaaa gtacctgtga gtaaaagtgg
    14701 gtatataagg cttaagcagg ttttgggagt gtgagggaat cccgcttggg cgggatgtcg
    14761 ctgcgctcca tgtgggaatg tatccgaaat ttgtatcagg tatcaagtat caggtatcaa
    14821 gtatcaagta aaaattcacg agtcacgatt cacgattcac gattggtagt gagtagtgag
    14881 acccaaatag atctgcaact tcgttgcata gattgaactt tgttttagaa taaaattact
    14941 ccctctccct ttgggagagg gccggggtga gggcactcac ccgcccgcct aaacggcgag
    15001 gtaaactcac ccactcaccc actgacccac tgacccactg acccactcac ccactcaccc
    15061 actcaccccc tctctcctct ttcctctctc ctttctccta aaaaaaaaga gccccaatcg
    15121 gagctcttct tttaataatt agcctaagta taatgcggac taaacgataa cattacatca
    15181 ttccatccat tcctccgggc attccgccgc ccattggttc tttcttttca ggtttatcta
    15241 cgatggtgca ttcggtggtg aggagcagac ctgcaattga ggctgcattt tcgagtgcaa
    15301 cacgtgttac tttcacagga tcaataacac ctgctttgag aaggttttca tatttctcgg
    15361 tgcgggcatt aaatccgaaa tcagcttttc cttcgcgtac tttttgcaca acgatagatc
    15421 cttcgatacc ggcgttacca acgatagtac gaataggcgc ttcgagtgaa cgtttgatga
    15481 tttcgatacc gattttttca tcttcgtttc caccacggat tttatccaac gcttcaattg
    15541 cacggatata cgcaacacca ccacctgcaa cgatgccttc ttcaacggca gcgcgtgtag
    15601 catgcaatgc atcatctacg cgatctttct tttctttcat cgctacttca gttgcagcac
    15661 caacatacaa tacggcaaca ccaccgctca attttgcgag acgctcttgc aatttttcac
    15721 gatcgtagtc agaagtagtt ttttctatct gcgctttaat ttcattgata cgcgctttga
    15781 tatcatcttt ctttccttta ccgccaacga tggtagtatt gtctttatcg atggttacac
    15841 gttctgcgcg accgaggtaa gtgaggtcag cattttctaa tttataacct tgttcttcgc
    15901 tgataacagt tccacctgta aggatggcga tatcttgcaa catttcttta cggcgatcgc
    15961 caaatccggg agctttaact gcacaaactt taatggttcc gcgaatttta tttactacga
    16021 gtgtagagag tgcttcacca tctacatct
//
LOCUS       DAOQOU010000058        15937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1851_length_15937_cov_6.698590, whole genome shotgun sequence.
ACCESSION   DAOQOU010000058 DAOQOU010000000
VERSION     DAOQOU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_15937_cov_6.698590"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLM90_06795"
     CDS             complement(<1..572)
                     /locus_tag="PLM90_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HMZ89114.1"
                     /translation="MKIKLICVCSLVMIKLSALTGQNALQIPPALYGPVYDLNVQNGV
                     VEFIPGITTPTYGINGNILGPTLFADKGDVVTLNVTNSLTGTGNSTTMHWHGFHLPAS
                     ADGGPHQVIDQGDTWSPSFTVMNDASTLWYHPHGDGKTDLQVARGLAGFFIIKDEVEN
                     ALTLPRTYGVDDIPLAIQTKAFDVLHQIAIA"
     gene            complement(583..1365)
                     /locus_tag="PLM90_06800"
     CDS             complement(583..1365)
                     /locus_tag="PLM90_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89115.1"
                     /translation="MKKSLAFCIMLMFAMHFNGIAQTAFQFTGEDCNGNAVDLYSDLD
                     AGKAAILLFYMPDCGACPPPASEIQEMANNVMNDFPDMIKGYVFPFLDIYDCPSVVSW
                     VDDSHVPFFTPMDSGEYQVAYYGGFGMPTVVLLGGTDHRVMFSTLSYLTSDTTIMKDS
                     ILRLFGVETAIEPVLDELKDLIVRPNPAYALISVSAAEMPIGEYVLTLVDINGAVMLN
                     QMGVSDGKLNATLDIAEISSGLYMLNITGANMHVSKSIMISH"
     gene            complement(1380..2249)
                     /locus_tag="PLM90_06805"
     CDS             complement(1380..2249)
                     /locus_tag="PLM90_06805"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89116.1"
                     /translation="MQFKNFITVIFSSLAIVSSAQIPNYSFEDWTTIDSYSMPVQWDC
                     LNALTETAAMFTCEQGTPGSPGSSFLKLTSKNIPGMGVMPGVAVSGVIDTITYAPISG
                     FAFTDRPESLTGKWQHMIYGGSQGYIDVVLTRWDLTSGERVTVASAHKQLTGMAMSWA
                     SFSIPLDYADDNFPDSCIITLRASGNSPSQNDFLYIDNLQFEGTVVSTGVINHNSTLQ
                     IFPNPAHDVLYISDASDLDFDDIVVVDAIGNICHVSTSLQGNNKIMLDCSGLTPGIYV
                     AHILSENIKFLVN"
     gene            complement(2316..2708)
                     /locus_tag="PLM90_06810"
     CDS             complement(2316..2708)
                     /locus_tag="PLM90_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89117.1"
                     /translation="MVTGKHLRLPIFLVLAFFAYSYVCSGYCQITAAISPSHDDEMAD
                     CCAKASPDQPTDEGNCQTKHIVFYNITGQFFVDHQVIIHPDINIQLEVSEIVPLPQAD
                     ISRLVEITNTGPPFSGAFTRIKVSSFQI"
     gene            complement(2856..4085)
                     /locus_tag="PLM90_06815"
     CDS             complement(2856..4085)
                     /locus_tag="PLM90_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89118.1"
                     /translation="MITSQAVIVVCILLLLAYIIDISAKHTKIPSVILLLALGFGVGR
                     LTQWLYIPVADLEPILPVLGTIGLILIVLEGALDLELNNSKFPAIRLALVNALLPILL
                     LAAIITTMFMFFWQLPLRVALVNAIPFCVISSAIAIPSVRGLSVFQREFVIFETSLSD
                     IIGVLFFNFILYNEVIDGHAFGHFGLQMLLIIVISFLAVLCLSFLMAKLSHHVTYTPI
                     LILIILIYYVSKIYHLPGLIFILVFGLFLGNLDEIKKFRWVEKFNPAKLDAEVHKFKS
                     ITLEATFIVRVLFFMVFGFLLKPEEIINLQTLPFAAGIVGLILLIRYVSLRISRLPIQ
                     PLLFIAPRGLITILLFISITGKLTSSHVTSAMIVQTVLLSVIIMMLGLMFYEPVEQNV
                     GTVTNKEARPNAHEAHE"
     gene            4378..4728
                     /locus_tag="PLM90_06820"
     CDS             4378..4728
                     /locus_tag="PLM90_06820"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HMZ89119.1"
                     /translation="MRVEVLKSKIHRVRVTESNLNYIGSITIDEDLLDAANMIEFEKV
                     QVLNINNGARLETYIIKGERGSGVICLNGPAAHHVTPGDIVIVCSYASMEWEEAKSWE
                     PTLIFPDSQTNMLR"
     gene            4730..5761
                     /locus_tag="PLM90_06825"
     CDS             4730..5761
                     /locus_tag="PLM90_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ89120.1"
                     /translation="MSRKLIFNILKFAIFLGIGVTLIWYITKDLTADQKHELWASLSA
                     ANYWWVGFSVLIGIASHIARALRWKMMLEPLGTNPRLSTTFHAVMIGYLANMAVPRLG
                     EITRCGIVQRYEKVPFDKAVGTLVVERSIDLLCLIIATGILFITQFTLIFDFFDEKVI
                     FPISQKLQFSTAFIIILAAIIAAISFGIWFFIKKFSHTEAYLRIRILILNVRDGIYSI
                     RHMKNFKLFLFYTFFIWFCYFSSAWVALYALSQTSGFGPNEALTILVFGTVGIISTPG
                     GIGAYHLIVTETLVALYGLDRTYAISFSWLAWSCQTIMIIFVALISLLAISRLSKPQQ
                     DEQASGHTA"
     gene            5733..6227
                     /gene="rfaE2"
                     /locus_tag="PLM90_06830"
     CDS             5733..6227
                     /gene="rfaE2"
                     /locus_tag="PLM90_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HMZ89121.1"
                     /translation="MSKPLAIQHKIMNLHTLVQQISVWRFSNRKIVFTNGCFDVLHKG
                     HAHVLNTASNLAYNAAVIVGVNSDASVKRLKGNDRPVNNEADRMLMLASMYAVDAVVL
                     FHEDTPYELINAIQPDFLVKGGDYAEDQIVGADIVKSRGGKVVVVPFLDNYSTTLLIE
                     KMKH"
     gene            6374..7513
                     /locus_tag="PLM90_06835"
     CDS             6374..7513
                     /locus_tag="PLM90_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMZ89122.1"
                     /translation="MYFQLTEEQQMIRQAARDFAQNVLKKGVIDRDRDMIYPYEEVKQ
                     MAELGFLGMMVSPEYGGGGMDTMSYVLAMEEISKVDSSCSVIMSVNNSLVCWGLEQFG
                     TEEQKRKYLPDLASGRKIGAFCLSEPEAGSDATSQRTTAEDKGDHYLLNGTKNWITNG
                     GTASIYLVMAQTHVEKGHKGINCLIVEKGMDGFIVGAKEDKLGIRASDTHTLLFNDVK
                     VPKENRIGDDGFGFTFAMKTLSGGRIGIASQALGIASGAYELALEYAKSRKAFGKAIA
                     DHQAIQFKLADMATEIEAARLLIYKAAQLKDAHENYDLASSMAKVFASEVAMKTTVEA
                     VQIHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQRIVIGRSILK"
     gene            complement(7584..8297)
                     /locus_tag="PLM90_06840"
     CDS             complement(7584..8297)
                     /locus_tag="PLM90_06840"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HMZ89123.1"
                     /translation="MFKRFFFVTVFMIAAGMLLQSFAGQTIRIEGSDVSISDFTTLNV
                     DVPFDVYFTQDAVVSAKVEGLDNIAQTIEFQQKGKELTLKLKEKGGHYGKVKVFLSAP
                     SLENVNVTKSGSFYTLNQIVNSKSFHAAIAGSGNVDIDANAPKVEIGIAGSGNAKLKG
                     SSKRVDVDIAGSGDCRGKDMLADEVSVDIAGSGNAYVFANDTVDVDIAGSGSVYYSGD
                     PEHVTTDIAGSGNVGPDKL"
     gene            complement(8397..9776)
                     /locus_tag="PLM90_06845"
     CDS             complement(8397..9776)
                     /locus_tag="PLM90_06845"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HMZ89124.1"
                     /translation="MTSVIILASAGIISMFVGIFNAKRLALPLVLLACLAALGIVMFR
                     YNGGYDGLLNNMLVFDDYSHGFNIVMILLTMGVLTISRYYYKDNIEHLSDIYALFIFS
                     LIGAILSVSFQNLIMLFIGIEILSIPLYILATSNRRNLFSNEAGLKYFLMGSFASCFL
                     LLGITFIYGTAYSFDMNAIGRFAADNATALPPMFVVGCVLILGAFIFKISAVPFHFWA
                     PDVYQGSPTVITSFMATVVKTAGFGALLRFLNLTIINNPGVWQEIVQVIAILTLVVGN
                     ILALYQQNLKRMLAYSGIANAGYVLVAVASAGEPTTGFVLYYLASYGVASLLAFAVYT
                     IVKDQTGTDTIEGLKGLFAQNKLLTITLALAMLSLSGIPPLAGFFGKYAIFANALSTG
                     MVWPVIIAVLGSLVGVYYYLRVMAVSFSGGEMNKVHVSPGYAFVLLLTAAILVALGVM
                     PDLFTELIA"
     gene            complement(9776..11245)
                     /locus_tag="PLM90_06850"
     CDS             complement(9776..11245)
                     /locus_tag="PLM90_06850"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMZ89125.1"
                     /translation="MLTLLLILLPIISGLIVFVAPRSASKQIAMISALLSLALGIGVY
                     AQFDASIGTQFAIDVPWIDMLGMRFHIGIDGISMLMIMLSNILMPLIILSTSKDNYEN
                     SALLYGLMLIMQGAMNGVFVALDMFLYYIFWELALVPAYFLVLWWGHGNARKITFKFF
                     IYTLFGSLFMLVAIIWLSLQSNDPSTDISSIYNVVIPENVQGYIFFAFMLAYAIKIPV
                     FPFHTWQPDTYTSAPSPATMLLSGVMLKMGIYSIIRWVIPVMPAAVEEYQTTIIVLAS
                     IGVLYASAIAWVQKDLKTLFAYSSIAHVGLITAGILTASASGLQGGMIQMVAHGINAV
                     GLFFVCQILFRAYGNHTISNMGGIRNNAPLFAGLFLVVMLASVALPLTNAFPGEFLLL
                     NSLYQFNPWLCLLAGSGVILGAVYMFNAYKNVMLGEAEGRSKSFVEISGADKLTLVII
                     AGLIFALGLFPQTITGLTEHSVHDIIMQYQSKISGTILR"
     gene            complement(11249..13159)
                     /gene="nuoL"
                     /locus_tag="PLM90_06855"
     CDS             complement(11249..13159)
                     /gene="nuoL"
                     /locus_tag="PLM90_06855"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HMZ89126.1"
                     /translation="MSQNALNLITLLIPALPLLGFVIHILFRDKISEKIAGYHATAWI
                     VISFVCSLILFNHVRSEGAIHVQLFEWFSAGDLSIPFAFQVDQLSVIMLLVITGIGSL
                     IHMYSIGYMHGDDGFNRFFSYLNLFIFFMLTLVMANNYVLMFVGWEGVGLCSYLLIGF
                     WFKNPEYNKAANKAFIMNRIGDLAMLLGLFLLYQYAGTLTFDGVSARFDPSSPDYFAP
                     DTHMVTAITMLLFIGACGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIVR
                     SNLMFSEAELTMQVITVVGIVTSIFAGAIALKQNDIKKVLAYSTVSQLGLMFFAIGVG
                     AYEAAMFHLVTHAFFKALLFLSAGSVIHGMGGEQDIRKMGGLRSYMKVTHWVFGFGVL
                     AIMAVPPFSGFFSKDTILAAAYVKSPVLWVIGLVSGLFTAYYMMRLYAVVFQGQPRMS
                     EHTKAHIHESPSVMTIPLMVLCALSIVGGLMNIPELFGGNKSMMHFLEGVVPIHEHHV
                     AVSTEWMLMAIASVAIFLVIFIAHRKYRINWVVPESDAEMSGAGKVLNHKFYVDELYE
                     MAIVKPMHMLSNFFYKIIDLKVIDGVVNGTAQFTTWCAGQLRLIQTGTVSFYLFMMAL
                     GMGAIVLFIYLM"
     gene            complement(13196..13528)
                     /gene="nuoK"
                     /locus_tag="PLM90_06860"
     CDS             complement(13196..13528)
                     /gene="nuoK"
                     /locus_tag="PLM90_06860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HMZ89127.1"
                     /translation="MDNTLSQQLQVIPLENYVMLSIILFSIGVIGILMRRNLIVVFLS
                     IELMLNSINLMAASFSAYHNDPAGQAFVFFIMVVAAAEVAVGLAMLVMIYRNVRSIEI
                     DALSRLKW"
     gene            complement(13521..14024)
                     /locus_tag="PLM90_06865"
     CDS             complement(13521..14024)
                     /locus_tag="PLM90_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMZ89128.1"
                     /translation="MTQVFFYGLSALAIASSLMMLFQRNAMYSVLYLLVCFFSVAGLY
                     IMLNAQFLAVVHIIVYAGAIMVLFLYVLMFLNLNTPAAFSKSNMLRMAAMLTGGSIFL
                     LLFSAFYRTQLIVMPDPMNENVGSVQTLGKVLFNDYLIPFELTSVLFLSAMVGVVIIN
                     KKDKVNG"
     gene            complement(14058..14606)
                     /locus_tag="PLM90_06870"
     CDS             complement(14058..14606)
                     /locus_tag="PLM90_06870"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HMZ89129.1"
                     /translation="MQSLTNRSKVVSNKHLSLAERLYLPAIFGGLRITFKHMFKKKAT
                     ISYPEEQRDHGRLYRGQHVLKRDEQGRENCTACGLCAVACPAEAITMVAGERKKGEEH
                     LYREEKYAIEYNINMLRCIFCGLCEEACPKDAIYLTDRIAESNYQRGQFIFTKDKLVE
                     GIDKSDRIDVSDRRKAVRKGAM"
     gene            complement(14641..15675)
                     /gene="nuoH"
                     /locus_tag="PLM90_06875"
     CDS             complement(14641..15675)
                     /gene="nuoH"
                     /locus_tag="PLM90_06875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMZ89130.1"
                     /translation="MDPILIFKLVLVFVLFAVSLGIAAYSTYAERKIAGFMQDRLGPN
                     RAGPFGLLQPLADGVKMFMKEDIIPETSNRFLFIAGPAITMITALMTSAVIPWGPEIT
                     INDVKVPLQVADINIGVLYVLGVVSLGVYGIMIGGWASNNKYSLLGAVRASSQMISYE
                     LAMGMSIIAIVMSSSSLSLGDIVAGQSGWGGMHWNIFIQPLGCLIFIICAFAECNRTP
                     FDLPESETELVGGYHTEYSSMKLGFYLFAEYVNMFISSAMISCLYFGGYNFPGMEAVQ
                     GNMGEIPFALICFVVLFAKILFFVFFFMWIRWTIPRFRFDQLMNLGWKVLFPLAILNV
                     LITGAVIIFF"
     gene            complement(15723..>15937)
                     /locus_tag="PLM90_06880"
     CDS             complement(15723..>15937)
                     /locus_tag="PLM90_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89131.1"
                     /translation="QGEEVLRVTSRKDKWGESEGFICNTCRFDKKNTSDWTIEGPAPV
                     DRHSVISQNHYELPYELPVHQKKLSN"
BASE COUNT         5140 a         3416 c         3031 g         4350 t
ORIGIN      
        1 gcaatggcta tttggtgtaa tacatcaaat gctttcgttt gtattgcaag cggaatgtcg
       61 tcaacaccat aggttcgtgg tagcgtcaat gcattctcga cttcatcctt aataataaaa
      121 aatcccgcca atccccgtgc cacctgcaaa tcagttttac catcaccatg agggtgatac
      181 cacaatgttg aagcatcatt cattaccgta aatgacgggc tccaggtatc tccctgatca
      241 atgacctgat gtggtccacc atcggctgat gccggcaaat gaaaaccatg ccagtgcatt
      301 gtagttgaat ttccggttcc ggtgagcgaa tttgtcacat ttaatgttac cacatcgcct
      361 ttatctgcaa ataacgttgg acctaatata ttaccattaa tgccataagt gggtgtggta
      421 atgccaggaa taaactcaac tacaccgttt tgcacattga gatcgtacac cggaccatat
      481 aatgcaggtg gaatttgcaa tgcattttgc cctgttagcg ctgataattt aatcatcact
      541 aaactgcaga cacaaatcaa tttaattttc atgctattcc gtttaatgac tgatcataat
      601 cgatttactg acatgcatat ttgctcctgt aatatttagc atatacaaac cggaggaaat
      661 ttcagcaata tccaatgtag catttagttt tccgtcactc acgcccattt gatttaacat
      721 tacagctcca ttgatatcaa ccagcgtcaa aacatactct ccaattggca tttcagcagc
      781 gctgacagaa atcaaggcgt aggcaggatt tggacgcact atcaaatctt ttaattcatc
      841 aagtacaggt tcaattgccg tttcaactcc aaataatcgt aaaatagaat ctttcattat
      901 ggtggtgtcg cttgtcaggt agcttaaggt actaaacatc accctgtgat cggtgccgcc
      961 aagcaaaaca actgttggca tgccgaagcc cccataataa gccacctgat attcgccact
     1021 atccataggt gtgaaaaatg gcacatggct gtcatctacc catgaaacga ctgaaggaca
     1081 atcataaatg tcaaggaagg gaaacacata accttttatc atgtcaggaa aatcattcat
     1141 cacattatta gccatttcct gtatttcaga agcgggtggc gggcacgctc cgcaatcagg
     1201 catataaaaa agcagaattg ctgctttacc tgcatccaga tcactgtaaa ggtcaactgc
     1261 attgccattg cagtcttctc cggtaaattg aaaagctgtt tgagcaatac cattgaaatg
     1321 catggcgaac atcaacatta tacaaaaagc gagactcttt ttcataaaaa atcaattaat
     1381 taatttacta agaatttaat attttcagat agaatatgtg caacataaat tccgggagtt
     1441 aagcctgaac aatcaagcat tatcttatta ttaccttgta atgaagtgct cacatggcaa
     1501 atatttccaa tggcatcaac aactacaatg tcatcaaagt ccagatccga cgcatcggag
     1561 atatacaaca catcatgcgc cggatttggg aaaatttgca gtgttgaatt gtgattaatt
     1621 acccctgttg atacaacagt tccctcgaat tgaaggttgt cgatatataa aaagtcattt
     1681 tgcgacggtg aatttccact ggcacgcaag gtgatgatac aagaatcagg aaaattatca
     1741 tcagcataat caagtggtat tgaaaatgaa gcccaactca tagccatgcc ggtcaattgt
     1801 ttgtgtgcag aagccactgt aactcgttcg cctgaggtta agtcccaacg agtaagaacg
     1861 acgtcaatat atccctggct tccaccataa atcatatgct gccatttccc ggtaaggctt
     1921 tcaggtctgt cagtaaatgc aaatcctgaa atgggcgcat aagtaattgt atcaataaca
     1981 ccgctaacgg caacgcccgg cataacgccc attccgggaa tatttttact ggtgagtttt
     2041 aaaaaactgc tacccggtga gccgggtgtt ccttgttcac aagtgaacat tgcagcagtt
     2101 tcagttaatg cattcagaca atcccattgt acaggcatgg aatagctatc aatagttgtc
     2161 caatcttcaa atgagtagtt gggtatttgt gccgatgaca caatagctaa agaggagaaa
     2221 attacggtga taaaattttt gaattgcata ataagtagtt tacagatgaa aaaataaata
     2281 ccccttctta tgaaaaggaa ttactcatct gaaaatcaaa tttgaaaact acttactttt
     2341 attcttgtaa aagctccgct gaacggcgga cctgtatttg ttatttctac taagcgtgaa
     2401 atatccgcct gaggtaaggg tactatttca gatacctcca attgaatgtt gatatcagga
     2461 tgaatgatta cctgatggtc aacaaaaaac tggccggtaa tattataaaa tacaatatgt
     2521 ttagtttggc aattgccctc atccgtaggt tggtcaggtg aagctttagc acagcaatct
     2581 gccatttcat cgtcatgtga tggactgatt gcggctgtta tctggcaata tccggaacac
     2641 acgtagctgt aggcaaaaaa tgccaagacc aagaaaatcg gcagtctaag atgtttcccg
     2701 gttaccataa gctatgtcaa aattagcgaa ttgaaagttt gctaccaaat acaatttccc
     2761 ccggccaaca aatattgact tttggagcca tcctatggta aataacgaga aatacaactg
     2821 tatgcaagcc aaaaacgatc agtatctcta ggttgctatt catgagcttc atgagcgttg
     2881 gggcgggcct ctttattcgt taccgtgcct acattttgtt caacaggctc gtaaaacatc
     2941 agtcctaaca tcatgataat cactgacaac agcactgttt gtacaatcat agccgacgta
     3001 acatgcgacg atgttagttt tccggtgatc gaaataaaca atagtatggt aatcagccct
     3061 cttggtgcaa taaataaaag aggctgaatc ggtaaacggg aaatcctcaa gctgacatat
     3121 ctaatcagga gtattaatcc cactattccc gcggcgaatg gcaaggtttg caggttaatt
     3181 atttcttcag gtttcaacag aaatccgaat accatgaaaa ataacacgcg cacaataaag
     3241 gtagcttcta atgtaatgga cttaaattta tgcacttcgg catccagttt tgcaggatta
     3301 aatttttcga cccaacggaa ctttttaatt tcatcgagat tgccaagaaa aaggccaaac
     3361 acaagaataa aaatcaatcc cggaaggtga taaatttttg aaacataata gattaaaatt
     3421 atcagaatca aaataggcgt ataggtaaca tgatgtgata gtttggccat taaaaacgac
     3481 aaacacaaaa ctgccaaaaa tgagatcaca atgatcaaca gcatctgcag tccaaagtgc
     3541 ccgaatgcat gcccatcgat cacttcatta tacaaaataa aattaaaaaa caatacaccg
     3601 ataatatctg ataagctcgt ttcaaaaatc acaaattcac gttgaaaaac cgaaagtccg
     3661 cgcacgctgg gaatagctat agcactgctt atcacacaaa atggaatagc gtttacaagt
     3721 gcaaccctca atgggagttg ccaaaaaaac atgaacattg tagtaataat agcggctagc
     3781 aataaaatgg gtaataatgc attaacaagc gctaacctga tggcggggaa tttcgaatta
     3841 ttcagttcta aatcaagcgc accttccaaa acaataagta tcaatccgat ggttcccaaa
     3901 accggcagta tgggttcaag atcagcaacg gggatataca gccactgagt taacctgcca
     3961 acaccaaaac ctaatgccag caataaaata acagaaggaa tttttgtatg ctttgcactg
     4021 atatcaatta tatacgccaa taacaatagt atacatacta caataactgc ctgagatgtt
     4081 atcatttcga aaagatacaa agaaataatt ttaaaataga aataatattt ttcctttttt
     4141 agggaataaa cacaatatcc aaggagaatc atctaatgat gaatcatctt attgcacaag
     4201 ttatttaatt aatctcacct cactttttcg acttttaatt tatgttattt tttagcaaca
     4261 tcctttaaaa ttttggctat ccacacatca aaaccttaat gcagggttga cttgcacttt
     4321 attatcggca ataacacaag ggcacgaatg gaagcaaaac gctaactttg ccccgttatg
     4381 cgcgttgaag tgttaaagag caagatccac agggttaggg taaccgagtc gaatttgaac
     4441 tacattggta gtatcactat tgatgaggac cttctggatg ctgctaatat gatagaattt
     4501 gagaaggtac aagtgctcaa tatcaataat ggcgcccgtt tggagaccta tattatcaaa
     4561 ggtgagcgcg gttcaggtgt tatatgcctg aatggtcctg ccgcacacca tgtaacaccc
     4621 ggcgatattg ttattgtttg ctcttatgcc tcaatggaat gggaggaagc caaaagctgg
     4681 gagcctaccc tgattttccc tgacagtcaa accaatatgc ttcgctgatt tgagccgtaa
     4741 gctgatattc aatatcctta aattcgcgat tttcctggga atcggggtaa ccttgatttg
     4801 gtatatcacg aaagacctca ctgccgacca gaaacatgag ctttgggcat cattgagtgc
     4861 tgcaaactat tggtgggtgg gcttttcggt gctgataggc attgccagcc atattgccag
     4921 agccttgcgt tggaagatga tgctcgaacc actgggcacc aatcctcgcc tgagcactac
     4981 cttccatgcc gttatgatcg ggtatcttgc caatatggca gtgccgcgac taggtgaaat
     5041 aacccgctgc ggcatcgttc aacggtatga aaaagtgcct ttcgacaagg ctgttggaac
     5101 cctggtggtc gagaggtcta tcgacttgct atgtcttatc attgccacgg ggatactatt
     5161 cattacgcaa ttcaccctga tattcgactt ctttgatgag aaggtgatat ttccgatcag
     5221 ccaaaaactg caattcagca ccgcctttat aataatattg gcagcaataa tagccgcgat
     5281 tagctttggc atctggtttt ttattaagaa attttcgcat accgaagcat acctgcggat
     5341 tcgcattctg atactcaatg tgcgcgatgg tatttactct atcaggcaca tgaaaaactt
     5401 taaactcttc ttgttttata cctttttcat ctggttttgc tacttctcct ctgcctgggt
     5461 agccttatat gccttaagtc agacgagcgg ttttggtccg aatgaggcac taaccatcct
     5521 cgttttcggc accgtgggta ttattagcac gccgggaggc atcggcgctt atcacctgat
     5581 tgtgactgaa actcttgtag ccttatacgg gttagaccgc acttatgcca tcagtttcag
     5641 ctggttggca tggagctgtc agaccataat gattatcttt gttgcactta tctcattact
     5701 cgcaatttcg cgtttgagta agcctcaaca agatgagcaa gcctctggcc atacagcata
     5761 aaataatgaa tctgcacacc cttgtgcaac agatcagcgt atggcgattt tcaaacagaa
     5821 aaattgtttt taccaacgga tgtttcgatg tattacacaa aggacatgca catgtgctca
     5881 atactgccag caacctggct tacaatgcag cagttattgt tggtgtaaac agtgatgctt
     5941 ctgtaaagcg attgaaagga aatgatcgac cggtaaataa tgaagccgat cgcatgttga
     6001 tgcttgcatc tatgtatgcg gtggatgcgg tagtattatt tcatgaggat acgccttacg
     6061 aacttatcaa tgcaattcaa ccggattttc tagtaaaagg tggcgattat gcggaagacc
     6121 aaattgttgg tgccgacatt gttaaatcga gaggaggtaa agttgtggtt gttccttttt
     6181 tggataatta ttccaccacc ctattgattg aaaaaatgaa acactaacca ccacaattcc
     6241 aaaacctata aaacccaaca ccaaaaccaa aacccatacc tgttacctga tacttgatac
     6301 ttgatacctg atacttgata cctgatacct gatacctgat accaaataac cataacttaa
     6361 aaaaataaaa catatgtatt tccaattaac tgaagaacaa caaatgatcc gtcaggctgc
     6421 ccgcgatttc gcacagaatg tgttgaagaa aggcgtaatc gaccgcgaca gagatatgat
     6481 ttatccatat gaagaagtga agcaaatggc agaacttgga tttctgggca tgatggtttc
     6541 accggagtac ggtggtggtg gcatggacac catgagttat gtgttggcta tggaagaaat
     6601 ttctaaggtc gacagttcgt gctctgtaat tatgagtgta aacaattcat tggtttgttg
     6661 gggattggaa caatttggca ccgaagaaca aaagcgcaag tatttaccgg atctggcatc
     6721 gggaagaaaa atcggtgcat tttgtttgag cgaacctgaa gcaggcagtg atgcaacatc
     6781 gcaacgcaca acagcagaag ataaaggtga tcattattta ttgaacggaa caaaaaactg
     6841 gatcaccaat ggtggcactg caagtattta tttggtgatg gcgcaaacgc atgtcgagaa
     6901 aggacataag ggtattaact gtcttatcgt agaaaaaggc atggatggat ttattgttgg
     6961 cgctaaagaa gataaattgg gtattcgcgc aagtgataca catactttat tgttcaacga
     7021 tgtaaaagtg cctaaagaaa atcgcattgg tgatgatgga ttcggattta catttgccat
     7081 gaaaacattg agtggtggtc gtattggtat tgccagtcag gcattgggaa ttgcatcagg
     7141 tgcatatgag cttgcattgg aatacgcaaa atcacgcaaa gcatttggaa aagcaattgc
     7201 cgatcatcaa gccattcaat ttaaacttgc tgacatggca acagaaattg aagctgcgcg
     7261 attacttatt tacaaagcag cacaattaaa agatgcgcat gaaaattacg atttagcatc
     7321 cagcatggcg aaagtatttg catcagaagt tgccatgaaa actacggttg aagcagttca
     7381 aattcacggt ggttacggat acgtaaaaga atatcacgta gaacgattga tgcgcgatgc
     7441 taaaatcact caaatctatg aaggcacaag tgaagtgcaa cgtattgtta tcggaaggag
     7501 tattttgaaa taatatttca ataaatgaaa actaaaaagg gaatcgtgtt gcgattccct
     7561 ttttttattg gatagatttt cggttataat ttatcaggtc cgacattgcc gcttccggca
     7621 atatcagttg taacatgctc aggatctccg gaataataaa cgcttccact tcccgcaata
     7681 tcaacatcaa cagtatcatt tgcaaaaaca tatgcatttc cggagcctgc aatgtcgaca
     7741 gaaacttcat cagccagcat atcttttcca cgacaatcgc cactgcctgc aatatcaaca
     7801 tcaacacgct tgctgcttcc tttcaatttt gcatttccgg aaccggcaat acctatttca
     7861 acttttggtg catttgcatc tatatcaaca tttcctgaac cggcaatagc tgcgtgaaat
     7921 gatttcgaat taacgatttg atttaaggtg tagaaactac cggacttggt aacattcaca
     7981 ttttcgagtg atggtgccga cagaaaaacc tttacttttc catagtgacc acccttctct
     8041 tttaatttca gtgtcaactc tttccctttt tgctgaaact ctatcgtttg tgcaatattg
     8101 tcgagtcctt caactttggc acttacaact gcatcctgcg tgaagtacac atcaaaaggc
     8161 acatcaacat tcagggtagt gaaatcactg attgaaacat cagagccttc aatgcggatt
     8221 gtttgtccgg caaatgattg caacagcata ccggctgcga tcatgaaaac agttacaaag
     8281 aaaaatcgtt tgaacatagc agttattttg ttgattaaaa gtaactacta taactgttga
     8341 agcaaagcct ttgttactta gaggcccaca ctttaaggaa atttaacgcg ttttaatcag
     8401 gctatcagct cagtaaagag gtcaggcatt actccgagcg ctaccaggat agcagctgtc
     8461 aataatagaa caaacgcata accggggctt acatgcacct tattcatttc tccgccactg
     8521 aaagatactg ccatcacacg caggtaataa tacacgccga ccaaagagcc caacacagct
     8581 atgatcaccg gccaaaccat gcctgtagaa agtgcatttg caaaaattgc atatttcccg
     8641 aagaatccgg ccagtggagg gatacctgag agagacaaca ttgccaatgc caaggtgatt
     8701 gtcaataatt tattttgtgc gaagaggcct tttaatcctt ctatcgtatc agttccggtt
     8761 tgatctttta caatagtata aaccgcaaat gcaagcagac tggcaacgcc ataagaagcg
     8821 aggtaataaa gcacgaagcc tgttgtcggc tcaccggctg aggcaacggc aacaagcaca
     8881 tatccggcat ttgcaattcc ggaataagcc aacatacgtt tcagattctg ttgatacaat
     8941 gcaagaatat ttcctaccac caaggttaaa atggcaatta cctgcacaat ctcctgccac
     9001 acgcccggat tattaatgat agtcaggttc aaaaatctta gcaacgcacc aaatcccgca
     9061 gttttcacaa cagttgccat aaatgaagtg attacagttg gagaaccctg atatacatcc
     9121 ggtgcccaaa aatgaaatgg tactgctgat attttgaaaa tgaaagcgcc taaaatcaac
     9181 acacatccta ccacaaacat tggaggcaat gcagtagcat tatcggcagc aaatctgcct
     9241 atcgcattca tatcgaaact gtaggcagtt ccataaataa aagtaattcc cagtaataag
     9301 aaacaagaag caaaggaacc cataaggaaa tatttcaacc ctgcttcatt gctaaataaa
     9361 ttgcggcgat tgctggtagc gagaatatac aatggtatac ttaaaatctc aataccgata
     9421 aacagcatga tgaggttttg aaatgaaacc gaaagtattg caccgatcag tgaaaaaata
     9481 aataaggcat agatatctga aaggtgttcg atattgtctt tataataata tctgctgata
     9541 gttagcacac ccatggtaag taatatcata acaatattaa atccatgcga atagtcgtcg
     9601 aacaccagca tgttgttgag cagtccgtca taaccgccat tatatctgaa catcacgatg
     9661 cccaatgctg ccaaacatgc aagcaataca agcggaagcg ccaaacgttt tgcgttgaat
     9721 atccccacaa acatggagat gatacctgca gatgctaaaa tgattactga tgtcattatc
     9781 ttaaaattgt tccactgatc ttagattgat attgcatgat aatatcatgc actgaatgtt
     9841 cggtaagccc tgtaattgtc tgagggaata atccgagtgc gaatattaat ccggcgatga
     9901 tcactaaagt aagtttatcg gcaccggaaa tttcaacgaa tgatttactt ctaccttcag
     9961 cttctccaag catcacattt ttatatgcat tgaacatgta aactgcgcct aaaatcacac
    10021 cgcttcccgc taacaggcag agccagggat tgaattgata taaagaattg agcaataaga
    10081 attcgccagg gaacgcattt gtaagcggca atgcaacgga tgccagcatt accaccaaaa
    10141 acaatcctgc aaatagcggt gcattatttc taattccgcc catattgcta atggtatggt
    10201 tgccataagc acggaataat atctgacaaa caaagaataa accaacagca ttgataccat
    10261 gcgccaccat ttgtatcata cctccttgaa gacctgatgc gcttgcagta agaattccgg
    10321 cggtaatgag accaacgtga gcaattgatg aataggcaaa taatgttttc aaatcttttt
    10381 gcacccatgc aatagcactt gcatacaata cacctattga tgcaagaaca ataattgtag
    10441 tttgatattc ttccactgct gcaggcataa caggtatcac ccagcgtatg atactataaa
    10501 tacccatttt taacatcaca ccggaaagca acattgttgc gggcgatggt gcacttgtat
    10561 aagtatccgg ttgccaggta tgaaacggga ataccggaat tttaatggca tacgccagca
    10621 tgaaagcaaa aaagatatat ccctgaacat tctcaggtat aacaacatta taaattgatg
    10681 aaatgtcggt tgaaggatca ttactttgga ggctaagcca gataatcgcc accagcataa
    10741 acaaacttcc aaacaaggta taaatgaaaa atttaaaagt gatttttctt gcatttccat
    10801 gcccccacca taacaccaaa aaataagcgg gcaccaatgc aagttcccag aatatgtaat
    10861 acaagaacat atccaaagca acaaaaacgc cattcattgc tccttgcatg atcagcatta
    10921 gaccgtataa taatgcgcta ttttcataat tatctttcga agttgataag atgatcagag
    10981 gcattaaaat atttgacaac atgatcataa gcatgctgat gccatcgatt cctatatgga
    11041 aacgcatccc cagcatatca atccaaggta catcaatagc gaactgtgtg ccgatggagg
    11101 cgtcaaattg cgcataaaca ccgattccca aagccagcga caaaagagcc gaaatcatgg
    11161 cgatttgttt tgatgcagat ctaggtgcaa caaaaacaat cagtcccgag atgattggca
    11221 gtaatatgag taataaggtc aacatccgtt acatcaaata aatgaataaa acgatagccc
    11281 ccataccgag agccatcatg aataaataaa agctcaccgt tcctgtttgt atcaatcgca
    11341 attgaccggc gcaccaggtt gtaaattgtg cagtgccgtt cacaacgcca tcaattacct
    11401 tcaggtctat gatcttataa aagaaattgc tgagcatatg cataggtttt actatggcca
    11461 tctcatacaa ttcatcaaca taaaatttat gattgagtac ttttccggca ccgctcattt
    11521 ctgcatcact ttccggtaca acccaattaa tacgatactt gcgatgtgca ataaatatca
    11581 ctaggaagat ggcaacgctg gctatagcca tcagcatcca ttccgtagaa acagcaacat
    11641 gatgttcatg tattggcacc actccttcaa ggaaatgcat cattgattta ttgcctccaa
    11701 ataactcggg aatattcatt aaaccaccca caattgacaa tgcgcaaagc accatcaacg
    11761 ggattgtcat cacacttgga gattcatgga tatgtgcttt agtatgctcc gacatgcgcg
    11821 gttggccttg aaataccact gcatacaagc gcatcatata atacgcagta aataagccgg
    11881 aaactaaacc tatcacccat aatacaggtg acttcacata ggctgcagca agtatggtat
    11941 ccttagaaaa gaatccacta aagggtggca ctgccataat tgctaaaaca ccgaaaccga
    12001 acacccaatg ggttaccttc atgtaactgc gcagtccgcc catttttcgt atatcctgtt
    12061 caccacccat tccgtgaata actgaaccgg cagacaagaa taataatgct ttaaagaaag
    12121 cgtgtgttac aaggtgaaac atagctgctt catatgcgcc gacaccaatc gcaaaaaaca
    12181 tcaaacccaa ttggctgact gtggaataag cgagtacttt tttaatgtcg ttttgtttca
    12241 atgcaattgc tcctgcaaaa atcgacgtaa caataccaac tacagtaatc acctgcatgg
    12301 taagttctgc ttcactgaac attaaattac tgcgcacaat catataaata cctgcagtaa
    12361 ccattgttgc agcatggatg agtgccgaaa caggcgtagg acctgccatg gcatcaggta
    12421 accaggtata taatggtatc tgcgcacttt taccgcatgc accgataaac agcaacatgg
    12481 taattgccgt aaccatatgg gtatccggag caaaataatc gggtgaggaa ggatcgaagc
    12541 gagcacttac accatcaaaa gtgagtgtgc cggcatactg atacaataaa aataaaccta
    12601 atagcattgc cagatcgccg atgcgattca taatgaatgc tttgttggca gctttattat
    12661 attcaggatt tttaaaccag aaaccaatca gtaaatatga acacaagcct acaccttccc
    12721 atcccacaaa catcaacaca tagttgtttg ccattaccag cgtgagcata aaaaagataa
    12781 agagattcag atagctgaaa aagcgattga atccatcatc accatgcata tatccaatgg
    12841 aatacatatg gatgaggctg ccaatacctg tgatcaccag taacatgata acactcaact
    12901 gatctacctg aaatgcaaat ggtatgctga ggtcacctgc cgaaaaccat tcaaacagct
    12961 gcacatgaat agcgccttca gaacgaacat ggttgaacag gataagtgag cacacaaatg
    13021 aaatcactat ccatgctgta gcgtggtagc ctgcaatttt ttcagaaatt ttatctctga
    13081 ataaaatatg tatcacaaat cccagcaaag gaagcgcggg gataaggagg gtaattaagt
    13141 ttaatgcgtt ctgactcacg tgaaaatatt gtcgtttact atattattta tttcgttacc
    13201 atttcaggcg gctcaatgcg tctatttcga tactgcgtac atttcgataa atcatcacca
    13261 acatggccag tcctacagca acttctgccg cagccacaac catgatgaaa aatacaaatg
    13321 cctgtccggc gggatcgtta tgatatgccg aaaatgaagc agccatcagg ttaatagagt
    13381 tcaacatcag ttcaatgctc aaaaaaacta caattaggtt tctgcgcatc aggataccga
    13441 tcacaccaat gctgaatagt atgatcgaca gcatcacata gttctccagt gggatcacct
    13501 gcaattgttg actgagtgta ttatccattt actttatctt ttttgttaat aatcactaca
    13561 cccaccatag ccgagaggaa taatacactc gtaagttcga atgggatgag gtaatcgtta
    13621 aataatacct taccgagcgt ttgtacactg cccacatttt cattcattgg atcgggcatt
    13681 acaatcagtt gtgtgcgata aaaagcagag aacaacaata aaaatattga tccacctgta
    13741 agcatcgctg ccatccgcaa catattactc ttcgaaaatg ccgcaggcgt gttcagatta
    13801 aggaacatta acacataaag gaacaatacc atgatggctc cggcataaac aatgatatgt
    13861 accaccgcca gaaactgcgc attgagcatg atatataatc ctgcaactga gaaaaagcag
    13921 accaaaagat acaacacgct atacatggcg ttgcgctgaa acagcatcat cagactactt
    13981 gcaatggcaa gcgccgataa gccgtaaaaa aatacctggg tcatcaaaat gtgttccgtt
    14041 ttactgattc taataattta catcgcacct ttgcgcaccg ctttcctacg gtcgcttaca
    14101 tcgatgcgat cacttttatc tattccttcc actaatttgt ctttagtgaa aataaattgt
    14161 ccacgctgat aattactttc tgcaatacga tcagtaagat agattgcatc tttagggcaa
    14221 gcttcttcac ataatccgca aaaaatgcaa cgcagcatat taatattata ctctatggca
    14281 tacttttctt cgcgatacag atgttcttca ccttttttcc tttctccggc aaccattgtt
    14341 atcgcttcgg ccggacatgc aacagcacac aagccgcatg cagtacagtt ttcacgccct
    14401 tgctcgtcgc gtttcaaaac gtgctgaccg cgatataatc taccatgatc acgctgctct
    14461 tcagggtaac tgatagtcgc cttcttctta aacatatgtt taaaggtaat ccgcagtcca
    14521 ccgaatattg ccggcagata taagcgttct gcaagcgaaa gatgcttgtt tgaaacgact
    14581 ttacttctgt tggttaaact ctgcatgtta tttttctgct tttcggtcta cactattcaa
    14641 ttaaaaaaat ataattacag ctccggtgat caatacattt aatattgcca acgggaataa
    14701 tactttccat ccgaggttca ttaactgatc aaaacggaat ctcgggatgg tccagcggat
    14761 ccacataaaa aagaatacaa aaaagaggat cttggcaaat agcacaacaa aacaaattaa
    14821 tgcaaatggt atttctccca tattcccttg caccgcttcc attccgggaa aattatatcc
    14881 gccgaaataa agacaagaaa tcatggcact tgagataaac atgttcacat attctgcgaa
    14941 cagataaaat cccaatttca ttgaactata ttccgtgtga tatccgccaa ccaattctgt
    15001 ttcagattcc ggaaggtcga atggtgttct gttacattcg gcaaatgcac aaataataaa
    15061 tattaaacaa cctaatggct gaataaaaat attccagtgc ataccacccc aaccactttg
    15121 tccggccacg atatcaccaa ggctgagaga actgctgctc atgacaattg ctataatact
    15181 catgcccatt gccagttcat aacttatcat ttggctagat gcgcgaacag cgccgagtaa
    15241 agaatattta ttattcgaag cccatccgcc aatcatgatg ccataaacac caagactcac
    15301 tacccctaat acatacaaca caccaatatt aatatcggca acctgcaatg gcactttcac
    15361 atcgttaatg gtaatttccg ggccccatgg aataacggcg ctcgtcatta atgcagtaat
    15421 catggtgatg gcaggaccgg caataaataa aaatctgttt gatgtttcag gaataatatc
    15481 ttctttcatg aacattttca caccatccgc caatggttgc aataaaccaa atggacctgc
    15541 tctgtttgga cccaggcgat cctgcataaa acccgcgatc ttgcgttcgg catatgtact
    15601 atatgctgca ataccgagcg atactgcaaa gagcacaaac acaagaacta atttaaatat
    15661 gagtattgga tccatttttt attactattt tttcgattcc tctaattata aactattagg
    15721 tatcagttac tcaatttttt ctgatgaaca ggtaattcat aaggtaattc gtaatgattc
    15781 tgcgaaatca ccgaatgacg atctaccggc gcaggacctt cgatggtcca atcagaagta
    15841 ttctttttat cgaatctaca tgtgttacaa ataaatcctt cgctttcacc ccatttatct
    15901 tttctgctgg ttacgcgaag cacttcttca ccctgat
//
LOCUS       DAOQOU010000059        15636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1902_length_15636_cov_6.077723, whole genome shotgun sequence.
ACCESSION   DAOQOU010000059 DAOQOU010000000
VERSION     DAOQOU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15636
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_15636_cov_6.077723"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..972)
                     /locus_tag="PLM90_06885"
     CDS             complement(34..972)
                     /locus_tag="PLM90_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ89132.1"
                     /translation="MKISNESKVGILAAVGIALLFIGFNYLKGINVFSKGIDYNVQYE
                     TSGGLLVGDPVIIDGLAVGRVKSVTLQQDQSGVDVILNFEQRLKIPADSYALIRGNLM
                     GEKYVQMFLGDSSELIPDGGTLTGKIESDLANTISQEFKPITDKVKTMLSSMDTAINV
                     LRGIFTEEVQNDFAKSMASIKTTLESFNNSADRVNALINKEESNIDSIITDVSGFTQT
                     VNQSKDEMTQILDNLKLISDSLKTVEWQSLAGEFQKAADNINAISAKINSGDGSLGML
                     VNDQQLYTDLTKTLTTLDSVLNEFGKDPTLRIKFFK"
     gene            complement(1024..2196)
                     /locus_tag="PLM90_06890"
     CDS             complement(1024..2196)
                     /locus_tag="PLM90_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ89133.1"
                     /translation="MKKQISRTSVITGAFMVLWLGVCAFNTPSTPELPAYKLKTVVID
                     AGHGGHDIGCSGSANNEKTVALKLALKLGEMIEKNYPDVKVIYTRKTDVFVELYERAA
                     IANRNNADLFICIHCNANPNSSPYGTETYVMGLHKTEANLNVAKRENDVILMEDNYNQ
                     HYDGFNPNDPASHIIFSLNQHAFMDQSILFASSVEKYLKSHAKRSSRGVKQAGFLVLW
                     KTAMPSVLIETGFLTNSSEEKFLASESGQTTIAESIFYAFRDYKNDMENNSGKVSEAT
                     VQAIEANMDKEVETPKTEPVTTSTDNKGLTFSVQFYTAESEASATKFADLKPENVTYV
                     KENGLYKYRLGPYKTLDEATKKQSFARNAGYKDAFVIAVKDGKRISIEEAKALAGS"
     gene            2267..4933
                     /locus_tag="PLM90_06895"
     CDS             2267..4933
                     /locus_tag="PLM90_06895"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HMZ89134.1"
                     /translation="MSRKFFITIWLLFAIGMAIGAHSGNFVYNNVQTNEAFVYSSAQS
                     VPDSSIAIDSTKNDSLLLNGNDLWYAVSPDALDQIINYKGVDSIIYDLDSGKTYIYAK
                     GEITYQSFNLKADFIVFDWAEKTICAKQITDSTGKKGDLVYFKDGDEEFKAEYMCYNF
                     GTKKGKIHYFRAQEGEGYIAVEQAKKNDDDSYYGDHLTYTTCELDHPHFYITAEKAKV
                     VPQKVAVTGPANLVIADVPTPLFLPFGIFPIKRGQTSGIIIPQYGNLTTQGFFLKGGG
                     YYFALSDYYDLMLTGDIYSRGSWGLHTSSRYNLRYKFNGALYLDYAVNKYDFPFSPNY
                     SENTGYYIRWSHSQDAKSTPNSKFSASVNLGSTNYLKDNSYSSSYLSNSLNSSISYGH
                     NFVGTPFNFTAALRHSQNISEKTVNATLPEAALTMNRIYPLKKLTDNKNSFMYQLGVT
                     YSMNMQNSVSRPDSLFFDQATLDAMRSGFQHKVSASAPVKVLKYFTFSPNVNYTENWY
                     FESIRKRYDPYTVTDTIQTGTGEDSIITTEYLVQVDTVEGFSAARYYSMGASLNTKIY
                     SLAQFNGKLKAIRHVMTPTLSFNYTPDFSLPKYGYYGEYYPTASQEEPTLYSIYEGGV
                     YSGPGRGEMGTISLGIGNTLEMKVYSKKDSVTHEKKVKLLENFSVSGGYNLAADSLNF
                     SDIGFSAYTTLFKNIRINVNGSFDPYILDTLGRNIDQFEWDVNHRIGRFNSGNISLST
                     DFQSKRNNNPSLATNAGTEEEREMVWNDPSAYIDFEVPWSFAVGYNLRVTNAPTIDGR
                     DSIYTTQSATFNGDLSLTPNWKVQVTTGYDVQQKEFTYTSIDIYRDLHCWQMSFKWIP
                     FGLRQSYVFNINVKASVLQDLKLTRKKDWSEY"
     gene            complement(4952..7042)
                     /locus_tag="PLM90_06900"
     CDS             complement(4952..7042)
                     /locus_tag="PLM90_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89135.1"
                     /translation="MRKVILPTLLLIATVSSFGQASFDHTVIEVGNLGMYITNAGTLG
                     RPDVRNTPTGDPSMEYPINSGIEHLFEGGFWIGAIVDGQYLVSTSAKDAASGYTTGLA
                     GYEFTSEIGNTIAQRSSLTSSDYFNFDAISHQDLLVDISDKNVIVPGTAITIADHTQP
                     LYADIHLETYAWNYSFADYFVLLNYTITNNSTSVWDSVWCGIWNDMVVRNVNVSTDYG
                     SAFFSHGGYGYLGDDFTNYAFDVDGDPGFTNAYGAFQFLGIEWRDQFIHPNNNDLLTA
                     NGYPAAEVNSNFWIFNSTATPPYNAPANDVERYDKLKQGLNYADPDIVEFLKDPSTTG
                     GLTNLLSAGPMISVEPGETFHVVFAAVCAKQIENGGTTGVDMDTPEAQAELLEHLSWS
                     KRTYLGEDQNENGLLDIGEDLDADTILDRYILPEPPATPKMKVIADDQRIDIYWDNRA
                     EFSVDPISKEMDFEGYRLYRTQPGDDFNLNLIGDANMIAQWDLPANGIGFNNGFSAIA
                     LTTPEIIDEDTFYYKYTLDPVLNGWQYLIILTAFDRGDEDLHIESLESSFIENAVHVF
                     PGTSPNNDANTEIGVYPNPYRLQAAWDGSTGTTGKIMFYNLPEHCTISIMTLSGDIVA
                     ELNHDAATYTGSDSEWFATYAGNEDERIFSGGEHAWNLLSESNQTITQGIYLFSVKDL
                     DNGNIKQGRFAIIK"
     gene            7227..9674
                     /locus_tag="PLM90_06905"
     CDS             7227..9674
                     /locus_tag="PLM90_06905"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HMZ89136.1"
                     /translation="MQNRMVIFAVMKKYICIHGHFYQPPRENAWLEIIEQQESAAPYH
                     DWNERINAECYGPNAASRILNDEQRIIDIVNNYSRISFNIGPTLISWIAQHDSDTYNA
                     ILQADKESLTHFDGHGNAIAQVYNHIIMPLASERDKETQVLWGIADFRHRFGRHPEGM
                     WLAETAVDTATLEVLAHHNIAYTILAPRQAKAIRNMQDDSWMPVDENTIDTTRPYLVK
                     LPSGKSITVFYYNGRISRDVAFAGLLNSGKKFAEALSSNEIALDHPSLINIATDGESY
                     GHHHYRGDMALASCLDYLKKDEDVVITNYGQFLAESPPVYETKIHENSSWSCVHGVER
                     WRSNCGCTDGGNPGFHQLWREPLRNALNWLKDQADVIFEQELKTYTDDPWHLRNGYIT
                     VVLNRNEEEIDAFLLKGCGRKLQYEEKIRVIRLLEMQRHAMLMFTSCGWFFDEVSRIE
                     TKQIMQYADRVIQIAEHETSSTLKDQFILLLDKAPSNIEKYKTAAGVYVNEIHKQRLT
                     LTRVGIHHAVMSIFDNTMQQGFRLNYHVKPVFFEKLLSGDLVLVSGHLQLASTFTYAS
                     EQLFFSALYLGQHHVIGGYADKMDEDHFEEIFLNLRDAFRNSDISTITTTIHRVFPGT
                     FSINDVFHDEREFLIARILSADVHAAEDSYRAVYERTYTLMDMLRQQKKSIPPLLHAN
                     LIAVINADLKNWFTHPSGDLHKLDQLTEAAIKWEVTLDVEHIGHVAARRLYDMVLHLA
                     HAPHDMSLLENMSKIIIRLHELKVKYRLWKIQNKYFRIGRDFINNKAFMRTMGDEEYK
                     RWLLKFKAVGEQLKIRF"
     gene            9710..12160
                     /locus_tag="PLM90_06910"
     CDS             9710..12160
                     /locus_tag="PLM90_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HMZ89137.1"
                     /translation="MYDYDQIIASWYATRNWKQYDFQQELAAAYQQGYNGILNAPTGS
                     GKTFAMWLPIIADHMAKGNHQATSGLKVIWITPLRALAKDIRNALQEACDLFDMGWRV
                     EIRTGDISAKDKQAQKKQMPDVLIITPESLHVLLCQIGYETIFENLSCVVVDEWHELL
                     GSKRGVQVELAISRLRGIREDQKKQLRIWGISATIGNLVEAMEVLLGVDAPEHTSIII
                     RSEQKKQIDIHTVLPEHIDVLPWAGHIGLKLLPNIIDIIHRSTTTLVFTNTRAMAEIW
                     YHALLENDERLAGVMAMHHGSLSGEVRDWVEENLHNGALKAVICTSSLDLGVDFRPVD
                     TVIQIGSPKGVARFTQRAGRSGHQPGAVSTIYFIPTHALELVEISAIRAAMEENVIEK
                     RDPMVMSFDVLIQYMVTLSISGGFKSNELYREVKSTYAYHLMSPEEWQWLLQHITQGG
                     QTLQAYDEFNKVGRVGDVYRIMHKKAATRHRLSIGTIVSDTVVTIKLMNGGKLGSIEE
                     YFIASLKPGDVFWFAGHCLEFLMLREMTAFVKITKEQKAITASYMGGRMPLSSYMADM
                     MRKQLGQAAESNLHNFEYTHLQPLLDLQRDRSAIPAYDTILVETAESKEGLHIFIYPF
                     EGRVVHEVLAGLIAFRISKKQELSISVAMNDYGFELLCNHPVEINESVIRELFEIKGL
                     DDDVFQSVNATEMARRKFRDISVISGMVFQGYPGEQKRARHLQSSAQLIFDVLRKYEP
                     SNLLIKQAYDEILNDQMENARLRTALLRISKSKILIKHTDRFSPFAFPIVVDDLSRNK
                     LTSESLASRIQQMLRENK"
     gene            12219..12863
                     /gene="pdeM"
                     /locus_tag="PLM90_06915"
     CDS             12219..12863
                     /gene="pdeM"
                     /locus_tag="PLM90_06915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HMZ89138.1"
                     /translation="MPIAVSIKGNSFRLSPDFALYWQDLQWLIIADAHFSKETHFRKH
                     GIAIPAAILQEDLRRVDVLIRTYSPQRVVFLGDMFHSENNPGIDAFAKWRNGNNMPID
                     LVIGNHDILDRSRYADLNIDIHEDKIIANNIVLAHDDLSDIPEGHYLLHGHIHPCIRL
                     AGKAKQSMRLPCFWFGKEKGVLPSFGTFTGGHAITPNKTDQVFAIADAQIVRIN"
     gene            complement(12868..13434)
                     /locus_tag="PLM90_06920"
     CDS             complement(12868..13434)
                     /locus_tag="PLM90_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMZ89139.1"
                     /translation="MINYPHIPSAGHLLISEPFMADPNFKRTVVLLCEHMQDTGTVGF
                     ILNKSLNVKVCDALMDFDIVQSELFYGGPVAQDTLHYLHRFGDKIEESFHVIDDIYWG
                     GNFEQIGDMLREGTLNPDHIKFFLGYSGWSPGQLEEEMDEKSWLLSKAKGHYVFDIGR
                     SELWKTVLNDLGGDYKMITNYPEDPRLN"
     gene            13606..15579
                     /locus_tag="PLM90_06925"
     CDS             13606..15579
                     /locus_tag="PLM90_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HMZ89140.1"
                     /translation="MKKLLLSFALLSPLSIFAQLSIDALGTDVPVDLSDYAGAGFQPG
                     GGAGTLNSDIWSAEGFTDGAVDFGETNLTGDFARGIGGAGITTGGIYGLPMPDGNRIW
                     IQPTNDDFTPGSMTLRLQNNTGITLGGLNIAYSAWASNDQERANSLLCSYSYDGVTFT
                     PIPELDFISPEVSDLVLHQSDFSFLLTGITVDPAAMFYIRFTGDDVSGAGARDEFGIS
                     NISFTAFEPDAVPEYNFEPSDITIDESTPDGSFIFSISESADCSFSFGYDPAGTATPG
                     FDFGLMTFAVDFTAGGPTSQEIIFAIVDDFETEIDETAIVYVTSVTGACIAGGAPSST
                     ITIVDNDAVVPPIATFTTVGVDDDEAVGTITGTLEISETADCTIQLYLDGASTMENGL
                     DYTFLLPTFLTFTEGGPTSIDFEIPIIDDLEVEPTEMLLINMLPWTGTCVMGAIGDFE
                     INITDNDVEAPVYASFTFTGSEHNEGDGSTDIYVYLSEAADCTIEVTANASSTAVNGL
                     DYSTTLPAEFTFTAGGATSQTLTIDIINDLEIEGDEALNLDMAVTSGACAIGLYPNYA
                     LDIIDDDFESIQQTSGIITQMYPNPAFELLNIQTDISMDNWFIYDINGKLIRNGSIQS
                     REIQIDIHDLVAGTYQLRVSNANESAITNFVKQ"
BASE COUNT         4546 a         3173 c         3236 g         4681 t
ORIGIN      
        1 cctgctacat atgaagactt cattcagtca agttcattta aagaatttga tcctgagtgt
       61 aggatcttta ccaaactcat tgagtacaga gtcaagagtt gttagtgtct tggtgaggtc
      121 ggtgtacaac tgttgatcat taaccagcat tcctaaactg ccatcacctg aattaatttt
      181 agcacttatt gcattgatat tgtcagcagc cttttgaaat tcgcctgcca aagattgcca
      241 ttcgaccgtt ttgagtgaat cgctgatcaa tttcagatta tccaatatct gtgtcatttc
      301 atccttgctc tggtttacag tttgtgtaaa tcccgacaca tcggtaatga tactgtcaat
      361 atttgattct tccttattta ttaatgcatt gactctgtct gcagaattat tgaatgattc
      421 cagagtagtt ttaatactag ccatactttt ggcgaagtca ttttgtactt cctcagtgaa
      481 tataccgcgc aacacattaa tggcagtatc catagagctg agcatggttt tcactttgtc
      541 ggtaataggt ttgaattcct ggctaattgt attcgctaaa tctgattcaa ttttaccggt
      601 aagcgtgccg ccgtcaggga tgagttctga actatcaccc aaaaacatct gcacatattt
      661 ttcacccatc aggtttcctc tgatcaaagc atatgaatct gccgggattt taagccgttg
      721 ttcgaaattg agaataacat caacaccgga ttgatcttgt tgcagggtaa cagatttcac
      781 tcttccgacg gctagtccat caatgattac cggatcgcca accaataaac ctcccgaagt
      841 ctcatactga acattgtagt caatgccttt gctgaataca ttaataccct ttagataatt
      901 gaatccgata aacaacagtg cgataccaac tgctgccaaa atgcccacct tcgattcgtt
      961 cgagatcttc accctgctaa tttacgtgct aagttataaa atagttgcgt tgtagccttt
     1021 ggttcagctt ccggcgaggg ctttggcctc ctcgatagaa atgcgtttac catctttcac
     1081 agctatcaca aatgcatctt tataacccgc atttcgggca aaactttgct ttttagtggc
     1141 ttcatccagg gttttgtatg gaccaagtcg atatttgtag agaccgtttt ctttaacata
     1201 tgtgacattt tcaggtttta ggtcagcaaa cttagtggcc gaagcctcag attccgctgt
     1261 atagaactga acgctgaaag tgagtccttt gttatctgtg gatgtggtta caggctcagt
     1321 tttcggtgtt tcaacctcct tgtccatatt cgcttcaata gcttgaactg ttgcctcgct
     1381 tacctttccg gagttgtttt ccatgtcgtt tttataatcg cggaacgcat aaaaaatgct
     1441 ttcggctatt gttgtttgtc cgctttcaga ggctaaaaac ttctcctcac tgctgttagt
     1501 taggaatccg gtctctatga gcacacttgg catggcagtt ttccagagca cgaggaaacc
     1561 ggcttgttta acaccgcgac tgctgcgttt ggcgtgactt ttaagatatt tttctactga
     1621 tgatgcaaat aggatgcttt ggtccatgaa tgcatgctga ttgaggctga aaatgatatg
     1681 tgatgccgga tcattgggat tgaacccatc gtagtgctga ttgtagttgt cctccatcag
     1741 gataacatca ttctcgcgtt ttgcaacgtt taagtttgcc tctgttttgt gtaaacccat
     1801 cacataggtt tcagttccat agggcgagct gttgggattg gcgttgcagt ggatgcaaat
     1861 gaacaaatcg gcattgtttc gattggcgat tgctgctcgt tcatataatt cgacaaaaac
     1921 gtctgtttta cgggtgtaga tcaccttcac atcgggatag ttcttttcta tcatttcacc
     1981 cagctttagg gcaagcttta aggcaactgt cttttcattg tttgcacttc ctgagcatcc
     2041 gatgtcatga ccaccatggc cggcgtctat aacaacagtc tttaatttgt atgcaggcaa
     2101 ttcaggggta gatggggtat tgaatgcgca tacgcctaac cacaacacca taaaggcgcc
     2161 tgtgataaca ctggttctac tgatctgctt cttcataatt tccatagctt tgcacaatat
     2221 ttgctgtcat ttacgcattt actctaaaac tattccaaag ttacctgtgt cgcgaaaatt
     2281 ttttatcaca atatggctgc tattcgcgat cggcatggca attggagctc attccggcaa
     2341 ttttgtatat aataatgtgc aaactaacga ggcgtttgtg tattcctctg cacaatcagt
     2401 gccagattcc tcgatagcta tagattcaac gaaaaatgat tcgcttttat tgaatggcaa
     2461 cgacctttgg tatgccgttt cgcccgatgc actggatcag atcattaatt ataagggtgt
     2521 agactctatt atttatgacc ttgactcagg caaaacatac atctatgcta aaggagaaat
     2581 cacttatcaa tcgtttaatc tgaaggccga tttcattgtt ttcgattggg cggaaaagac
     2641 catttgcgca aaacaaatta ccgatagcac cggaaaaaag ggcgatctgg tttattttaa
     2701 agatggagat gaggagttca aagcggaata catgtgttac aatttcggca cgaaaaaagg
     2761 aaaaatacat tatttccgag cgcaggaagg tgaaggatat attgcagttg aacaggcaaa
     2821 gaaaaacgat gacgattcat actatggtga tcacttaaca tatactactt gtgaattaga
     2881 tcatccgcat ttttatatta cagcggaaaa agcaaaagta gtacctcaaa aagttgccgt
     2941 aaccggtccg gctaaccttg ttattgctga tgttccaacg cccttgtttc tgccattcgg
     3001 aatttttcct attaagcgcg gacaaacttc aggcatcatc ataccgcaat atggtaattt
     3061 gactacacag ggttttttcc tgaaaggtgg cgggtattat tttgccttga gcgattatta
     3121 tgatctcatg ttaacgggag atatttattc acgtggctca tggggattgc ataccagctc
     3181 gagatataat ttgaggtata aatttaatgg tgcactttac ctcgattatg ccgttaataa
     3241 atatgacttt cccttcagcc ctaattattc agaaaataca ggatattata tcagatggtc
     3301 gcatagtcag gatgctaaat caactccaaa ttcaaaattc agtgcaagtg tgaatctggg
     3361 gtctaccaat tatctgaaag ataactctta ttcaagcagc tatctttcga attcactcaa
     3421 ttccagcatt tcctatgggc ataattttgt tggaacacct ttcaacttta ccgctgcatt
     3481 gcgccacagt cagaatatca gcgagaagac agttaacgcc accttacctg aagcagcttt
     3541 gactatgaat cgtatttacc ctctgaaaaa gctgacagat aataaaaaca gctttatgta
     3601 tcagctggga gttacctatt ctatgaatat gcaaaattct gtttccaggc cggattcatt
     3661 atttttcgat caggcgacac ttgatgccat gcgaagcgga tttcaacata aggtatctgc
     3721 atctgcaccg gtgaaagttt taaaatattt cactttcagc cctaatgtaa attataccga
     3781 aaactggtat ttcgaatcca ttcgcaaacg ttatgatcct tatacggtaa ctgatacaat
     3841 tcaaaccggg acaggagaag atagtatcat taccaccgag tatttagtgc aggtagatac
     3901 cgttgaaggt tttagtgctg cacgatatta ttccatgggt gcctcgctga acaccaaaat
     3961 ttattcctta gcacagttca atggtaagtt gaaggcaatc aggcatgtga tgacacctac
     4021 attgtcattt aattacaccc ccgattttag tttgccgaaa tatggctatt atggtgaata
     4081 ttatccaacc gcttcacaag aagaacctac attgtattcc atttatgaag gtggtgttta
     4141 cagtggtccc ggaagaggtg aaatgggaac tatttcactt ggaattggaa acacgctgga
     4201 aatgaaagtg tattcaaaga aagatagtgt tacccatgaa aagaaagtaa aacttctgga
     4261 aaacttcagc gttagcggcg gatacaatct ggctgctgat tcactcaatt tttctgacat
     4321 cggatttagt gcctacacta ccttattcaa aaatattcgc atcaatgtga atggaagttt
     4381 tgatccatat attctggata ccttaggtcg gaatatcgat cagtttgaat gggatgttaa
     4441 tcatcgtatc ggtagattca atagcggtaa tattagtttg agtacagatt ttcagtccaa
     4501 gcggaataat aatccttcac tcgcaaccaa cgccggcact gaagaggaac gtgaaatggt
     4561 ttggaacgat ccgtcagctt atatcgattt cgaagtgcct tggagctttg ctgtcggcta
     4621 taatttacgt gtcaccaatg ctccaacaat agatgggcgt gatagtattt atactactca
     4681 gtcggcaaca tttaatggtg atttaagcct tactccaaat tggaaagtgc aggtaactac
     4741 tggttatgac gttcaacaaa aggaatttac ttacaccagt attgatattt accgtgactt
     4801 acattgctgg cagatgagtt tcaaatggat accattcgga ttaagacagt cttatgtctt
     4861 taatatcaat gtaaaagcca gtgtgctgca ggatttaaaa cttacacgta aaaaggactg
     4921 gtcggaatat tgagattcaa ttaattgatc atcatttaat aatagcaaat cgaccttgtt
     4981 tgatattccc attgtcaagg tctttcacgc tgaataaata aattccttgt gtaattgtct
     5041 ggttactttc gctcaacaag ttccaggcat gttctccgcc actaaatatg cgttcatctt
     5101 cgttgcctgc ataggttgca aaccattcac tgtcgctacc ggtataggtt gcagcatcgt
     5161 gattgagttc tgcaacaata tcgccactca atgtcatgat agaaatggta caatgttcag
     5221 gaagattgta aaacatgatc ttacctgttg ttcctgtgct tccgtcccag gcggcttgga
     5281 ggcgatatgg atttgggtaa acaccaattt cggtatttgc atcattattt ggtgaagtac
     5341 ccggaaacac atggacagca ttttcaataa aagaagattc caggctttca atatgaaggt
     5401 cttcatcacc gcggtcgaat gcagtaagaa ttatcaaata ttgccatccg tttaatacgg
     5461 gatcaagtgt gtatttgtaa taaaatgtat cctcatcaat aatttccgga gttgtaaggg
     5521 caatagctga aaacccgtta ttaaatccga taccgttagc cggaagatcc cattgagcaa
     5581 tcatattcgc atcaccaata aggttgagat taaaatcgtc accgggttgt gtgcgataca
     5641 atcgatatcc ttcaaaatcc atttcttttg aaatagggtc taccgaaaat tctgcgcgat
     5701 tgtcccaata aatatctatg cgctgatcat cggcaatgac tttcattttt ggcgttgccg
     5761 gtggttcagg taatatatat ctgtcgagta tggtatcggc atcaaggtct tcaccaatgt
     5821 ccaataatcc attttcattt tgatcttcgc caaggtaggt gcgcttcgac caactgagat
     5881 gttccaataa ttcggcttga gcttcaggtg tatccatatc cacacccgta gtgcctccgt
     5941 tttctatctg cttggcgcaa actgctgcaa atactacgtg aaaagtttct cccggttcaa
     6001 cacttatcat cggacctgcg gaaagtaaat ttgtaagtcc tcctgttgta ctggggtcct
     6061 ttaaaaattc aactatatca ggatcagcat aattaagacc ttgctttaat ttatcatagc
     6121 gttccacatc attggccggt gcattatatg gtggagtcgc cgttgaattg aatatccaga
     6181 aattactatt tacttcagca gcgggataac catttgctgt aagcaggtca ttgttgttcg
     6241 ggtgaataaa ttggtctctc cattctatac ctagaaattg aaaagcacca tatgcatttg
     6301 tgaaaccggg atcgccatca acatcgaatg cataatttgt aaaatcgtcg ccaagatacc
     6361 cgtatccgcc atgactgaaa aatgcggagc cataatcggt ggaaacattc acattgcgca
     6421 ccaccatatc attccagatg ccgcaccata cgctatccca aactgaagtt gaattatttg
     6481 tgatggtata attcagtaac acaaaataat ctgcgaagga ataattccat gcataagttt
     6541 ccagatgaat atcggcatac agtggttgag tatgatccgc aatagtgatg gcagtgccgg
     6601 gaacgatcac atttttatcg gaaatatcta ccaataaatc ctgatgtgaa attgcgtcga
     6661 aattaaaata atcacttgaa gtaagggatg accgttgagc aatagtattt ccgatttcag
     6721 atgtgaattc atatcctgca agtccggtag tgtaaccact tgcagcatcc ttggctgagg
     6781 tgcttaccaa atattgcccg tcgacaatag cacctatcca gaatccgcct tcaaataagt
     6841 gttcaattcc ggaattaatc ggatattcca ttgaagggtc accggtaggg gtattgcgca
     6901 catcaggacg accaagcgtg ccggcattgg tgatatacat tccgagatta cccacttcaa
     6961 ttactgtatg atcgaaggag gcctgtccga acgaactgac agttgcaatc aatagcagtg
     7021 taggtaaaat caccttgcgc atgggtcaaa gttacggaac cttaattaat tgcctcacac
     7081 ctcaacaatt gatgaaatct gttccctatg cattgtatat ggaagttgct tcaattttga
     7141 agtaaggatt cccgtgaaca ttacaataaa ttttgaaaaa catgcgcaaa tgtttccgat
     7201 tcgaatgatg attaatatat gctcaaatgc agaatcgaat ggttattttt gcagtaatga
     7261 agaagtatat ctgcattcac gggcattttt atcagccgcc ccgtgagaat gcatggctgg
     7321 aaattatcga gcagcaggaa tcggcagccc cttatcatga ttggaatgag cgcataaatg
     7381 cggagtgtta cggacctaat gcggcgtcac gtattctaaa tgatgagcaa cgaattatcg
     7441 atatcgtcaa taattacagc cgtatcagtt ttaatatcgg acctaccctg atatcgtgga
     7501 ttgcgcaaca cgacagtgat acctacaatg ccattttgca ggcagataag gaaagcctca
     7561 cgcattttga cggccacgga aacgccatcg cccaggtcta taatcacatc atcatgcccc
     7621 ttgcctctga gcgagataag gaaacacagg tgctgtgggg aattgcagat ttcaggcatc
     7681 gttttggacg acaccctgaa ggtatgtggc tcgctgaaac ggctgtagat accgctacgc
     7741 tcgaggtctt ggcacatcac aatatcgcat acaccatact tgcccctcgt caggcaaaag
     7801 ccattcgcaa tatgcaggat gatagctgga tgccggttga tgaaaatacc attgatacca
     7861 ctcgcccata tttagtgaaa ttgcctagcg gaaaatcaat taccgttttc tattacaatg
     7921 gcagaatatc ccgcgacgtc gcctttgccg gattgttgaa ttccggaaag aaatttgctg
     7981 aagcactgag tagtaatgaa attgcccttg atcatccttc attgatcaat attgccacag
     8041 atggtgaatc atatggtcat catcattatc gcggagatat ggccctcgct tcatgtctcg
     8101 attatctgaa aaaggatgag gacgttgtca ttacaaatta tggtcagttc ctcgctgaat
     8161 ccccacctgt ttatgaaaca aaaattcatg aaaacagctc ctggagttgt gtgcatggtg
     8221 ttgaacgatg gaggagtaat tgcggatgta ccgatggcgg aaatcccgga ttccatcaac
     8281 tttggcgcga acctttgcgc aatgcgttga actggttgaa agatcaggca gatgtcattt
     8341 ttgaacagga attgaaaaca tatacggacg atccatggca tttacgaaat ggttatatta
     8401 ccgtagtatt aaatcgcaac gaagaagaaa ttgatgcatt tttattgaag ggctgcggaa
     8461 gaaaattgca gtatgaagaa aaaataagag taattcgact gctcgaaatg caacgtcatg
     8521 cgatgttgat gtttaccagt tgtggatggt tttttgatga agtaagcaga attgaaacaa
     8581 agcaaatcat gcagtatgcc gacagggtaa tacaaattgc agagcatgaa acttcatcaa
     8641 ccctcaaaga tcaatttatt ctcttattag ataaggcacc ctcaaatatc gagaaatata
     8701 aaacggcagc tggtgtatat gtcaatgaaa tccacaaaca acgtttgacg cttacacgtg
     8761 ttggtattca tcacgccgtg atgagtattt tcgacaatac tatgcagcag ggatttcgat
     8821 tgaattatca tgtaaaacct gtattttttg aaaaattatt gtccggtgat ctcgtattgg
     8881 taagcggcca cttgcagctg gcgtccacat tcacttatgc cagcgaacaa ttgttttttt
     8941 cagcattata tctggggcaa catcatgtta ttggtggata tgctgataaa atggatgagg
     9001 atcattttga agaaattttc ctcaacctgc gcgatgcctt cagaaatagt gatatcagca
     9061 caattactac caccatacat cgcgtttttc cgggcacatt ttctatcaac gatgtatttc
     9121 acgatgagcg cgaatttttg atcgcacgaa ttttaagtgc tgatgtgcat gccgcagaag
     9181 atagctatcg cgcagtttat gaacgaacat atactttgat ggatatgctt cgtcaacaaa
     9241 aaaaatctat tccgccattg ttgcacgcca atttaatcgc cgtgataaac gccgatctca
     9301 aaaattggtt tacacatcct tcaggagatc tgcacaaact ggaccaattg acagaagcgg
     9361 ctatcaaatg ggaggtgaca ctggatgttg aacatatcgg acatgtggct gcacgaagat
     9421 tatatgacat ggtattgcat cttgctcatg caccacatga tatgtcactg ctcgaaaata
     9481 tgagtaaaat catcatccga ctgcacgaat taaaagtgaa atatcgcctt tggaaaattc
     9541 agaataaata tttccgcatt ggacgcgatt tcatcaataa taaagccttc atgcgtacta
     9601 tgggagatga ggagtataag cgctggttgc tgaaatttaa agcagtgggc gaacaattga
     9661 agatcaggtt ttaattgtat tttggactgc atttttgtgt gcctatatcg tgtacgatta
     9721 cgatcagatt atagcaagct ggtatgccac cagaaactgg aaacaatacg atttccagca
     9781 ggagctcgcc gcagcctatc agcagggata taacggaatt ctaaatgcgc ctaccggtag
     9841 cggtaaaact tttgccatgt ggctgccgat tattgccgat catatggcaa aaggaaatca
     9901 ccaagccaca agtggattaa aagtaatttg gataactccc ttacgtgcat tggcaaaaga
     9961 tattcgcaat gcacttcagg aggcctgcga tctttttgac atgggttggc gtgtggaaat
    10021 cagaaccggt gacatctctg caaaagacaa acaggcgcaa aaaaaacaaa tgcctgatgt
    10081 gctcatcatc actcccgaaa gtttgcatgt tttgttatgc cagatcggtt acgaaacaat
    10141 ttttgaaaat ctttcttgtg tcgtggtgga tgaatggcat gaattattgg gaagcaaacg
    10201 tggagtacag gttgaattag ctatcagcag gcttcgcgga attcgtgaag atcaaaaaaa
    10261 acaattgcgc atctggggga tttctgcaac cattggtaat ctggttgaag ctatggaagt
    10321 gctgttaggt gtggatgctc ctgagcatac aagtatcatc atccgcagtg agcaaaaaaa
    10381 acaaatcgat attcatacgg tattacccga gcatatagat gttttaccct gggcgggaca
    10441 tatcggatta aaattattac ctaatatcat agatattatt catcgttcaa ctactacgtt
    10501 ggtattcacc aatacccgtg ccatggctga aatatggtat catgcattat tagagaatga
    10561 tgagcgattg gctggcgtta tggccatgca tcacggcagt ttatcaggtg aagtgcggga
    10621 ttgggttgaa gaaaatttgc acaatggcgc attaaaggca gtgatatgca cttccagttt
    10681 agatcttggt gttgacttcc gacctgttga taccgtaatt caaattggtt caccaaaagg
    10741 tgttgcacga tttacacaac gcgccggaag aagcggacat caaccgggag ctgtttcaac
    10801 catttatttt attccgacac atgcacttga attggtagaa atttctgcaa ttcgagctgc
    10861 tatggaggaa aatgtaattg agaaacgtga tcctatggtg atgagttttg atgtattgat
    10921 tcaatatatg gtcacgctca gtatcagcgg cggatttaaa tccaacgaat tatatcgcga
    10981 agtaaaaagc acttatgctt atcatttaat gtcgcctgaa gaatggcaat ggttgctgca
    11041 acatataacc cagggcggac aaacattaca agcatatgat gaatttaata aagtcggcag
    11101 ggtaggagat gtatatcgca ttatgcacaa aaaagctgct acgcggcatc ggctttcaat
    11161 cggcactatt gtgagcgata cggtagttac aattaaactg atgaatggtg gcaagttggg
    11221 tagtattgaa gaatatttta ttgcatccct gaaacccggc gatgtatttt ggtttgccgg
    11281 acattgtctt gagtttctga tgctcaggga gatgacggca tttgtgaaaa ttaccaaaga
    11341 acaaaaagcg ataacagcat cttacatggg tggaagaatg cccttgtcga gttatatggc
    11401 agatatgatg cgcaaacaac ttggacaggc agccgaatca aacttgcata attttgaata
    11461 tacgcatctt cagcctttat tggatttgca acgcgacagg tcggccatcc cggcttacga
    11521 tactattctt gtagaaactg ccgaaagcaa agaaggactt cacatattta tttatccctt
    11581 tgaagggcgt gttgtgcatg aagtgcttgc gggtttaata gcattcagga ttagtaaaaa
    11641 acaggaactc agtatttcag tggccatgaa tgattatggt tttgaattat tgtgcaatca
    11701 cccggttgaa atcaatgaat ctgtgattcg ggaattattt gaaattaaag gcctcgacga
    11761 tgatgtattt caatctgtaa atgcaactga aatggcacga agaaaatttc gtgatatttc
    11821 tgtcatttcc ggaatggttt ttcaaggtta tcccggtgaa cagaaaagag ccaggcatct
    11881 gcaatcaagt gcacaattga tttttgatgt gctgcggaaa tatgaacctt caaacttact
    11941 cattaaacaa gcctatgatg aaatattaaa tgatcagatg gaaaatgcgc gacttcgtac
    12001 cgccttattg cgcatttcga aatcgaaaat tctgattaaa cataccgacc gcttttcacc
    12061 ttttgcattc cctattgttg ttgatgattt gtcgcgcaat aaactcacat cagaaagcct
    12121 ggcatcgcgg attcaacaaa tgttgagaga gaataaataa gtgttggcgt tgcatttctc
    12181 ctgtaaacta aatttatact aagtattacc tttgtctcat gccgattgct gtttccataa
    12241 agggcaattc ttttcgattg tcgccggatt ttgccctcta ttggcaggac ctgcaatggc
    12301 ttatcattgc cgatgcacat ttttctaaag aaacacattt caggaaacat ggcatcgcca
    12361 ttccggctgc aattttgcag gaggatctca ggcgagtaga tgtgctcatt agaacttatt
    12421 cccctcaacg agtagtattc cttggtgata tgtttcacag tgaaaataat cccggaatag
    12481 atgctttcgc gaagtggcga aacggaaata atatgcctat cgatctcgta attggcaatc
    12541 acgacatttt agaccgctca cgctatgctg atcttaatat tgatattcat gaagataaaa
    12601 taatcgccaa taatattgta cttgcacacg acgatttatc tgatatacct gaaggtcatt
    12661 atttgctgca tggacacata catccctgta ttcgcctcgc aggaaaggct aaacaatcca
    12721 tgcgattgcc atgcttttgg tttggcaaag aaaaaggtgt gttgccatct ttcggcacat
    12781 ttacaggtgg gcatgccatc actccaaaca aaactgatca ggtattcgca attgccgatg
    12841 cgcaaatcgt cagaataaac taaaatctta attcagcctc ggatcttcag ggtagttggt
    12901 gatcatttta taatcgccgc caagatcatt taatactgtt ttccataatt cactgcgccc
    12961 gatatcgaaa acataatgcc cttttgcttt tgataagagc catgattttt catccatttc
    13021 ttcctccaat tgtccgggac tccatccgct atatcccaaa aagaatttga tatggtcagg
    13081 gtttaaagtg ccttcgcgca gcatatcacc tatctgttca aaatttcctc cccaataaat
    13141 gtcatcaata acatgaaagc tttcttcaat tttatcgcca aatcgatgta aatagtgcaa
    13201 cgtatcctga gctacaggtc caccataaaa taattcgctc tgcacaatat caaaatccat
    13261 cagggcatcg caaactttaa cattcagcga cttattcaga ataaagccta ccgttccggt
    13321 atcctgcata tgttcacata acaaaacaac agttcgcttg aaattcggat ctgccataaa
    13381 tggttcactg ataagcaaat gtcctgccga aggtatatgt ggatagttga tcatgcttgc
    13441 tgcactgtcg gtttaagccc caaatatgca aagtgatcgg caatatgcca accgcttaac
    13501 atcaaattta tgctgcatat atacatattt catgcctgtt cagacccaag gatcccgcaa
    13561 atagtgctta tttttacacc tataccatta aaaaataccc ttctgatgaa aaaattgcta
    13621 ctctcatttg cactcttgtc tccactttca atttttgctc agctgagcat cgatgcgctt
    13681 ggaacagatg tgcctgttga cctgtctgac tacgccggag ccggattcca gcccggtgga
    13741 ggtgccggaa ccctcaattc tgatatctgg agtgcggaag gctttaccga cggtgcagta
    13801 gattttggcg aaaccaacct cacaggcgac tttgcccgtg gcatcggtgg ggccggaatt
    13861 acaacaggag gtatctatgg gctgccaatg cccgatggca acagaatttg gattcagcct
    13921 actaacgacg actttacccc tggttctatg accttgcgtt tgcagaataa taccggcatt
    13981 accttgggtg gacttaatat tgcatacagc gcctgggcat caaatgatca ggaaagagca
    14041 aatagtttac tctgttcata ttcgtatgat ggtgttactt tcacgcctat ccctgaattg
    14101 gattttattt ctcctgaggt gtctgacctg gttttacatc aatcagattt tagctttttg
    14161 ctgacaggta ttaccgtaga ccctgctgca atgttttata tccgttttac aggtgatgat
    14221 gtttccggag ccggtgcgcg cgatgaattt ggaatcagca atatatcatt taccgccttc
    14281 gaacccgatg cagtaccgga atataatttc gaaccttcag atattactat cgatgaatct
    14341 acacctgatg gatcattcat cttttctatc agcgaaagtg ccgattgctc attttctttc
    14401 ggttatgatc ccgcaggaac agcaacaccg ggtttcgatt ttggactgat gacatttgca
    14461 gttgatttta ccgcaggagg accaacatcg caggaaatta tttttgctat tgtagatgat
    14521 ttcgaaacag aaattgatga gactgcaata gtctatgtga cttccgtaac cggagcatgt
    14581 attgccggag gtgctccaag ttcaacaatt actattgttg ataatgatgc cgttgttcct
    14641 ccaattgcca ccttcaccac tgttggtgtt gatgatgacg aagctgttgg aacaattaca
    14701 ggaacattag aaatatctga aactgctgat tgtactattc agctttatct tgatggcgca
    14761 tctaccatgg aaaatggttt agactataca ttcttattgc caaccttcct cacatttact
    14821 gaaggcggac caacttctat tgattttgaa attcctatca tcgatgattt ggaagtagaa
    14881 cctactgaaa tgctgcttat caatatgctg ccatggacag gcacatgcgt aatgggtgct
    14941 atcggcgact tcgaaataaa tatcactgat aatgatgtgg aagctcctgt ttatgcatca
    15001 tttacgttta ccggatctga acacaacgaa ggcgacggtt caacagatat ttatgtctat
    15061 ttatctgaag ccgcagattg cacaatagag gtaactgcca atgctagctc tactgcagtt
    15121 aatggtttgg actacagtac aacattacct gctgaattta cattcaccgc aggaggtgct
    15181 acatcacaaa cattaaccat cgatatcatc aatgatcttg aaattgaagg cgatgaagca
    15241 ttgaatctgg atatggctgt cacctccgga gcatgtgcta tcggcttata tccaaattat
    15301 gcactggata ttattgacga tgacttcgaa tctattcaac aaacatccgg tattattact
    15361 caaatgtatc caaatccggc gtttgaatta ctcaacattc aaactgacat ttcaatggat
    15421 aattggttta tttatgatat caatggaaaa ttaattcgca acggaagtat tcaatcccgt
    15481 gaaatccaaa tcgatattca tgaccttgtt gcaggaactt atcaattgag agtttcgaat
    15541 gcaaatgaaa gtgctattac taattttgtg aaacaataat ttatttctga tgaagaaaat
    15601 tttactgaca attatggcat cagccggttt attact
//
LOCUS       DAOQOU010000060        15311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1960_length_15311_cov_5.441203, whole genome shotgun sequence.
ACCESSION   DAOQOU010000060 DAOQOU010000000
VERSION     DAOQOU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1960_length_15311_cov_5.441203"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLM90_06930"
     CDS             <1..244
                     /locus_tag="PLM90_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89141.1"
                     /translation="LLSSAFLCGKNLFYTLAFCVQILGLISPFIDKVLKRMNIHTFAL
                     RLVAYFYQMNMALLQGWWKYMTGIRSATWNPTQRNV"
     gene            258..1472
                     /locus_tag="PLM90_06935"
     CDS             258..1472
                     /locus_tag="PLM90_06935"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HMZ89142.1"
                     /translation="MEEIKLNTIPEAIEDIRQGKLIIVVDDENRENEGDFITAARNVT
                     PEIINFMATHGRGLICAPLIEDRCDELELNLMVDDNTALHSTQFTVSVDLIGHGCTTG
                     ISTADRALTIKALINPEMGAKDLARPGHIFPLRAKKGGVLRRAGHTEATIDLARMAGF
                     EPAGVLVEIMNEDGTMARLTDLVKVAEKFDLKIISIKDLIEYRLQTETIIHKEVEVQL
                     PTKYGHFTLHAYTQTTTGEVHMALVKGKWQNEEAVLVRVHSSCATGDILGSLRCDCGD
                     QLHAAMHMIDKNGSGVLLYMQQEGRGIGLLNKLKAYKLQEEGRDTVEANLELGFPMDQ
                     RDYGVGAQILRDLGIRKINLITNNPKKRVALKGYGLEIMGNTPIEILPNAHNENYLLT
                     KRDKMGHEILKD"
     gene            complement(1483..1881)
                     /locus_tag="PLM90_06940"
     CDS             complement(1483..1881)
                     /locus_tag="PLM90_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89143.1"
                     /translation="MVHTLQRHDSLEAALLAPIRDYLAGIYCPADGWKLFNRFQWRTK
                     VPDMIIQKQGKTGFERVIVSVHLENGASRQHFSEMEKLSSRLQKDTVGRMKKILVVDN
                     AAPLDLPADVEVVNLRDLLKIRATEPKLVA"
     gene            complement(2113..2265)
                     /locus_tag="PLM90_06945"
     CDS             complement(2113..2265)
                     /locus_tag="PLM90_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89144.1"
                     /translation="MSIIFAILMSLGIISNPVNSDVKHDGINQQKFDYTYSSNVKGGL
                     DWEDSH"
     gene            2455..3933
                     /locus_tag="PLM90_06950"
     CDS             2455..3933
                     /locus_tag="PLM90_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89145.1"
                     /translation="MEKITKLVHSLSNREVKLFYKYNALSGKKTNNLKIRLFEIAKQN
                     PEISDIEAAKILGKKVDAAFSMLKHRLQEDIFKVLVFTVDEAKFFSKTFKARYEVFKL
                     LAQSTLLDSRNLKQLSTEAIQKADRIAQEYELLSSRVIINEILINSHQAAQGFRTYNK
                     FKETVFSDISKQQALATGQDLFKQLIVPNMFAANRKKSQAEIAKNSAEQLEQLNKIHP
                     SVRLNFLLLRSQMEYAEITQDFQTYYNTASEFLKLVTNNPPVSTSDNIGGANMLLAES
                     SLRLHNWDNSKIFINESEKYFFKNSNNYITLMSIKYSMLVQTKSYEDAYQLVQEIRQL
                     KSIKRGSFQYSKWIYYEANIKFLQGDFQGSMNLLKRHSFLKSDKSGWRIGFKILEMLC
                     IIEIGHYDWLPYRLETFRKLLKDISKENVSRPTLIHKILLKLTRENFDFSTTDRKTFA
                     ELNLLRTDKGEYYRDPISYEIIPFDTWWDDKMATIKNRKKAE"
     gene            3966..4208
                     /locus_tag="PLM90_06955"
     CDS             3966..4208
                     /locus_tag="PLM90_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HMZ89146.1"
                     /translation="MKSYQPVACQWYDQLEALSVKGLELQVIYIDEQNVHHHLAGSIT
                     TLYTKDKAEYMVINDQVHIRLDKLISVNGILPGSYC"
     gene            complement(4211..5308)
                     /gene="ychF"
                     /locus_tag="PLM90_06960"
     CDS             complement(4211..5308)
                     /gene="ychF"
                     /locus_tag="PLM90_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMZ89147.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNALSSAKAQAANFPFCTIEPNVGVV
                     TVPDPRLNALSDLVIPERVVPTTIEFVDIAGLVKGASKGEGLGNKFLANIRETDAIIH
                     VVRCFDDPNIVHVHGNVDPIRDKEIIDYELQLKDLETIEVRITKTQRAAGAGDKDAKR
                     LMDVLTALKAHLEQGKSARSLNLDHNDLEVISDLFLLTIKPVIYVCNVAENELKTENA
                     FVQTLRNAVKEEEAEVIVVSANIESEIAQLESYDDKQLFLADLGLSESGLNVLIRAGY
                     KLLNLITYFTAGVKEVRAWTIHRGMLAPQAAGVIHSDFEKGFIRAEVIAYDDYLTYKS
                     EAACREAGKLRLEGKEYVVKDGDIMHFRFNV"
     gene            complement(5770..6234)
                     /locus_tag="PLM90_06965"
     CDS             complement(5770..6234)
                     /locus_tag="PLM90_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ89148.1"
                     /translation="MANTFTQTLVHVIFAVDGRDNLLTKEFNNQVYRYLTSFLNKEQA
                     IPHAVHCSLDHVHVLITLPPTKNLSRLIGDLKAYSSGYINANGWVEKVFRWQAGYVGI
                     SCSPMEREGMIAYVIGQDALHRKWSFKEEYFKLLDESGIVYDEQYGFEFQEG"
     gene            complement(6401..8656)
                     /locus_tag="PLM90_06970"
     CDS             complement(6401..8656)
                     /locus_tag="PLM90_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ89149.1"
                     /translation="MKKIIYSLLCILLVQWHSRAQVVVFSDDFEGGLANWTYTGLWNT
                     TTAYAYSPTTCFTDSPDGNYPDMFSSEAAMNVSADLSTALDADVQFMALVDLETAFDY
                     VYVDVSDDAGASWINVYVLNGEGMFTWTNYTIPIGAFVGSPDVRVRFRFESDAAYNVD
                     GLYIDDFKITKYNIDVSPPLILHTPTNLYEGNLEETSLTAEIIDASGISSATLYYNVD
                     GGAFTAVPGDLIGGDTYSFIIPTEAPGSWIDYYIEAIDDFGVPNTATTETYQIIAGNY
                     IGYDDATIDFVADIGTLSLTGYVSAAVKVTLAGSTDVVTAIIQNYTDYMRPNNDIRFH
                     VWADDGGFPGADLVTPFYVTPEPTLDEPNKGTRIDLRGVPELEGIFGDVFIGYDVPGG
                     VAWISYTGTGVINRSYVQTAFGWSEFFGDFHFRVVTSALTGAPMADFSVDYLATPTLN
                     FTDESTGSPTEWYWDFGDGGTSTLENPTHTYLVNGVYNVCLTATNMITSDTYCEAISI
                     NDYEVPVAAFSFTGDPTVTFTDLSSNLPTSWYWDFNDGTNSTEQNPVHTFLEDGSYFV
                     CLTATNAEGFNTNCQAVIIINTPKVPVVDFEWSLPGGTTVNFNDLSSNTPAYWEWSFG
                     DGGTSSEQNPSHFYPAPTSYTVCLTAGNVAGEATLCKVIDNFNAVGENGNSGMAISVF
                     PNPVTDHIMVVYSDVLPAVCTLRDALGNALLTQEIVGNTVIDMQSLPAGIYYIDVLRG
                     EAAFGISIMKQ"
     gene            8758..9573
                     /locus_tag="PLM90_06975"
     CDS             8758..9573
                     /locus_tag="PLM90_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89150.1"
                     /translation="MLKHTLFFAFLLTLYMPLHAQTADQWFATGYEKYEQQDYAGAIS
                     DYTECIMLAPDFPEAYYNRGLCNYFMGYYDAAIFDFNQVILQKPGEARAYYWKGYTQF
                     ALGHYEEAENAYSKALYYDPTYVAAFFERAFTRYTLGDFADAIEDFNKVLSIDHSNSD
                     AWFNRGLCFIAIADYTSALQDFSRVVESNAADPDAWYYIGFIYNKQLKFEEAVSALTK
                     VITLESDAVDALYQRGFAYYNLLQDEAACTDWSEGALLGNELCKELYEKECMK"
     gene            complement(9598..10197)
                     /locus_tag="PLM90_06980"
     CDS             complement(9598..10197)
                     /locus_tag="PLM90_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89151.1"
                     /translation="MRFVVLTLFVLQTAIVIGQNTEPITVSSDNDSVYFTMNGERSSI
                     ANTDMAAFYLEDGLVAANAGDFISAENKFRVGLLYDPENEQLWYNLGLAQYYQGAYED
                     AIYSFDAAVEVDPSNPENYNQRGLSKAMLGRYTDAETDFLILFKYAPDHPMGNFNYGI
                     LKLQMGDQATACTYLHKADSLGYENAPTVISTYCAMEGE"
     gene            complement(10331..11818)
                     /gene="accC"
                     /locus_tag="PLM90_06985"
     CDS             complement(10331..11818)
                     /gene="accC"
                     /locus_tag="PLM90_06985"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ89152.1"
                     /translation="MNKILVANRGEIALRVMRTAREMGIKTVAVYSEADRNAIHTRFA
                     DEAYFIGPPQSNQSYLLMDKILEVAKMSGADAIHPGYGFLSENAVFAEKVAEAGIKFI
                     GPSAYSINMMGDKISAKQAAKNYHIPMVPGTDHAITDIAEAKAVAKKAGYPILIKASA
                     GGGGKGMRVVNNEEELEEQMHRAMSEALSAFGNDAVFIEKFVTSPRHIEIQVLADDHG
                     NVVYLFERECSIQRRHQKLVEEAPSAVVSPEMRKAMGESAVMVAKACNYSGAGTVEFL
                     VDDQLNYYFLEMNTRLQVEHPVTEFITGLDLVREQILIAEGKALSFTQDDLRIHGHAI
                     ELRVTAEDPTNNFLPDIGKLITYRRPQGHGIRVDDGYEEGMDIPIYYDPLLAKLIVHA
                     QTRELACKKMIRAIQDYQISGVATTLPFGTFVMQHEAFLSGNFDTKFIERYFTPEALQ
                     LHDEEASAIAAFLAARLMESEKSAETQSAIATQTTKSKWLERKRM"
     gene            12229..12891
                     /locus_tag="PLM90_06990"
     CDS             12229..12891
                     /locus_tag="PLM90_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HMZ89153.1"
                     /translation="MQFWPSVWGMLKKDLILEMRQKYAISGILLYMLCIVFIIFMTFG
                     QGVRGPVWVVLFWLIMLFTSVNAVAKSFLQESTGRLLYYYTIASPQAIILAKMLYNIG
                     LMILMSALALLMHSITSGYPIVDSPSFLLANVLGAIAFATTFSMISAIASKASNSGTM
                     MAILSFPILIPILIILIRISLNAIQQNNLAVNLQDLMYLAALDVMILAMALILFPYLW
                     RD"
     gene            13042..13734
                     /gene="ccsA"
                     /locus_tag="PLM90_06995"
     CDS             13042..13734
                     /gene="ccsA"
                     /locus_tag="PLM90_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMZ89154.1"
                     /translation="MLKQLHNRWWKILCVAFLVYTFTAGLLIHTPDVPLLHETVRNLF
                     FHVPCWFSMLLLMLISLIYSIKFLRSGRMIHDVIAVSLLKVAMVFGVLGYLTGMLWGN
                     FIWGDLLSWITNDTKILGAAIGLLIYFAYFILRGSIDDEEKRARVAAVYSIFAFILLN
                     VSINVVPRLTSSLHPGSGGNATFTVYDLDNNLRMVFYPAVFGYTLLGLWISSIIIRMT
                     ILHYKKNDIPLV"
     gene            13731..13985
                     /locus_tag="PLM90_07000"
     CDS             13731..13985
                     /locus_tag="PLM90_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HMZ89155.1"
                     /translation="MKKLTSLSRFIATFLFLLANITFAGAQNQGYATTADSNENLLRS
                     NGLIYVVVGVIVIILSGLILYLVSIDRKLGKMEKEYGDKH"
     gene            14041..>15311
                     /locus_tag="PLM90_07005"
     CDS             14041..>15311
                     /locus_tag="PLM90_07005"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMZ89156.1"
                     /translation="MSNTSFFESVEKNFEKAAQITGHPRGLLDQIRVCNSVYHMKFPV
                     KIDGEFQVIEAWRVQHSHHRVPTKGGIRYDAMVNEDEVMALASLMTYKCAIVDVPFGG
                     AKGGIRINPRQFSSDQLERITRRYAAELCKKNMLGPGLDVPAPDYGTGEREMSWIVDT
                     YMALNPGSIDGYGCVTGKPVTQHGINGRREATGLGVFFGVREACSFADDMAELGLKPG
                     LEGKKVIVQGLGNVGYYSAKFFQDGGATIVGIGEYEGAIYNVNGLDVDAVFNHRKSTG
                     SILNFPGATNMANTAEILEQPCDILIPAALENVIHEGNAKKIKAKIIGEAANGPVTTE
                     ADDILNGMGVMIIPDMYLNAGGVTVSYFEWLKNLSHVRFGRLTKRFEQGSNMRIVDTI
                     EELTGKKLSQKEVDNIIKGPDEIDLVRSGLE"
BASE COUNT         4363 a         3480 c         3201 g         4267 t
ORIGIN      
        1 attgctgtcg tcggcctttt tatgcggtaa gaacttattt tatacgctgg cgttttgtgt
       61 gcagatactt ggtttaattt caccttttat cgacaaggtg cttaagcgga tgaatatcca
      121 tacctttgca ctccgattgg tggcctattt ctatcaaatg aatatggcct tgttgcaggg
      181 atggtggaaa tatatgacag gcatacgctc agccacctgg aatccgacgc aacgcaacgt
      241 ttaaacagaa tgaaacaatg gaagagatta agctgaatac tattcccgaa gctattgaag
      301 acatacgtca aggtaaactg attattgtgg tggatgacga aaatcgtgaa aatgaaggcg
      361 atttcattac cgccgcgcgc aacgttaccc ccgagatcat caattttatg gctacccacg
      421 gcagaggcct tatctgcgct ccgctgattg aagacagatg tgatgaactg gaattaaatc
      481 tgatggtgga tgacaatacc gctttacata gcacgcaatt caccgtatcg gtagatctca
      541 tagggcatgg atgcaccacg ggtatctcca ccgccgacag agcccttacc atcaaagcat
      601 tgataaaccc ggaaatgggc gccaaagacc ttgcccgtcc gggacatatt tttccgctgc
      661 gtgcaaaaaa gggtggcgta ttacgtcgcg ccggacatac agaggcaacc atagaccttg
      721 ctcgtatggc gggatttgag cctgcagggg tgttggtgga aatcatgaac gaagacggca
      781 ccatggcacg tcttaccgac ctggtgaagg tggcagagaa attcgacctg aagatcatca
      841 gcatcaaaga tcttattgaa tatcgcctgc aaacagaaac catcatccat aaagaagtgg
      901 aggtacaact gcccacaaaa tatggtcatt tcaccttaca tgcctacacc cagaccacta
      961 ccggcgaggt gcacatggcg cttgtgaaag gaaaatggca aaacgaagaa gctgtattgg
     1021 tgcgcgtaca cagcagttgc gccacgggcg atatcctcgg ttctttgcgt tgcgattgcg
     1081 gcgatcagct acacgcggcc atgcacatga tagacaaaaa cggctccggt gtattattat
     1141 atatgcagca ggaaggccgc ggcattgggc tattgaataa acttaaggct tacaaactgc
     1201 aagaggaagg gcgcgacacc gtggaagcca atttggagct cggcttcccg atggatcagc
     1261 gcgattacgg cgtgggtgct cagatacttc gcgacctggg catacgcaag attaatctca
     1321 tcaccaacaa tcctaaaaag cgtgtggcgc taaaaggata tggtttagaa ataatgggca
     1381 ataccccgat agagatattg cccaatgcac acaatgaaaa ctatctcctg actaagagag
     1441 ataagatggg ccacgaaatc ctaaaggatt aagggggtta aatcaagcaa caagcttggg
     1501 ttccgtggcc cgtatcttta acagatcgcg gaggtttacc acctctacat ctgccggcag
     1561 atcaagcggt gcggcattgt ctaccaccaa tatttttttc atgcgaccta cagtatcctt
     1621 ctgcaggcgg gaagaaagct tttccatctc actgaagtgc tgtcgtgaag caccattttc
     1681 aagatgaaca ctcacaatca ctcgctcgaa accggtctta ccctgtttct ggatgatcat
     1741 gtcgggcacc ttcgtgcgcc attgaaagcg gttgaataat ttccagccat ctgccggaca
     1801 atagatacct gctaaataat cccttatcgg ggcgagcaag gcagcttcca gggaatcgtg
     1861 tcgttgtagt gtgtgaacca tacccgtttc gtttaggcaa tgcttaattg caattgctga
     1921 tgctaagatc atgcgatacg gtggccgcca caaggaaaaa taatagcttc cctaacatcg
     1981 ttttttgaac aatgcaggtt tgtatcgtcc tgattttcaa gacaataggt ctctaatacc
     2041 gttaaataat taacagttta gtatatttga tgcatgaaat gatgcatcaa ctaagcacag
     2101 tgtaggtttc gattagtgag aatcttccca gtcgagtccg ccttttacat tagaagaata
     2161 ggtgtaatca aatttttgct ggttgatacc atcgtgttta acgtcagagt ttacagggtt
     2221 ggaaatgatt ccaagtgaca tcaggatagc gaagatgatt gacatagtgt tgcggtttag
     2281 aagtgttgag ttaaaatgaa tatgtttatt tttaatataa atagcaatta cagacccttc
     2341 aacagaatat gctgttgaca attaaacaaa tactcgtgtg tgtggttggg gtgtgcgtgt
     2401 gtaattaacg aacccaaaag tagaaattgt gactaaacaa ttcgtttcaa aagtatggaa
     2461 aaaataacaa aactagtgca tagtctttcc aaccgggaag tcaaactttt ctacaaatac
     2521 aacgccttaa gcggtaagaa aacgaataat ttaaaaattc gtctattcga aattgcgaag
     2581 caaaatcctg aaattagtga catagaagca gcgaaaattt tgggaaagaa ggttgatgcg
     2641 gcttttagca tgttaaaaca ccgtttgcag gaggatattt ttaaagtttt agtgtttact
     2701 gtagacgaag cgaaattttt ttcaaaaaca ttcaaagcga gatatgaagt ttttaaactt
     2761 cttgcgcaaa gtacattatt agacagcagg aatttaaaac agctttcaac cgaagccatt
     2821 caaaaagcag accgaattgc gcaggaatat gaattactta gttcaagggt aataataaat
     2881 gaaattctta ttaactctca tcaagcagct caaggtttca gaacttataa caaatttaaa
     2941 gaaactgtat ttagtgatat aagcaaacaa caagcacttg caaccggaca ggatcttttc
     3001 aagcagttga tagtccccaa catgtttgcg gcgaatagga aaaaaagcca agctgaaatc
     3061 gccaaaaatt cagcagagca actcgaacaa ttgaataaaa ttcatccctc agtaaggtta
     3121 aattttttgt tgctgcgaag tcaaatggaa tatgctgaaa taacacaaga ctttcaaact
     3181 tattataata cagcaagtga atttttaaag cttgtaacaa acaatccacc agtgtctaca
     3241 agcgataaca ttggaggtgc caatatgctg cttgcggaat cttcactgcg cctacacaac
     3301 tgggacaatt caaaaatatt tattaatgaa tctgaaaaat atttcttcaa gaacagcaac
     3361 aactacatta cattaatgtc aattaaatac tcgatgcttg tacagacaaa gtcatatgaa
     3421 gatgcatatc aattagtaca agaaatccgt caattaaaat ctatcaaacg aggaagcttc
     3481 caatattcca aatggattta ttatgaagca aacatcaaat ttctgcaggg agattttcaa
     3541 ggatcaatga acttgctaaa acgccacagc ttcctaaaat cagataaatc cgggtggcgc
     3601 atcggattta aaatactaga aatgctgtgc attattgaaa taggccatta cgactggctg
     3661 ccgtatcgcc ttgaaacttt ccgcaagcta ctgaaagaca tttccaagga aaacgtttcg
     3721 cgaccaacat tgattcataa aatactccta aaactcacac gagagaattt tgacttttct
     3781 acaactgaca ggaagacatt tgccgaatta aatctattgc gaacagataa aggtgaatat
     3841 tatcgggatc ctatttcata tgaaattatt ccattcgata catggtggga tgataaaatg
     3901 gcaactatta aaaatcgcaa aaaagcagag tgaaacgaat tatatattcc tgttgttagc
     3961 agtgcatgaa atcctaccaa cccgttgcct gtcaatggta tgatcagctg gaagcccttt
     4021 cagttaaggg tttagaattg caagttatat acatcgatga gcaaaacgtc catcaccatc
     4081 ttgccgggag catcaccacg ctttacacta aagacaaggc agaatatatg gtcattaatg
     4141 accaagtaca tatacgcctg gataagctga tatctgtgaa cggaatattg cccggcagct
     4201 attgctaggc ctacacatta aacctaaaat gcataatatc tccatccttc accacatatt
     4261 cctttccttc cagtcggagt ttaccggctt cgcggcaggc ggcttctgat ttgtaggtga
     4321 ggtagtcgtc gtaggcaatt acttctgcgc ggatgaagcc tttttcaaag tcgctgtgga
     4381 tcacccctgc cgcctgtggt gccagcatac cgcggtgtat ggtccaggcg cgcacttctt
     4441 ttacgccggc ggtgaagtag gtaatgaggt tcaatagctt gtatcccgca cggataagca
     4501 cattcagccc gctttcgctc agtccgaggt cggcgaggaa taattgcttg tcgtcatacg
     4561 attcgagctg tgcaatttca ctttctatgt ttgcggaaac cactattact tcggcttctt
     4621 cttcttttac ggcattgcgc aaggtttgca caaaggcatt ttccgttttc agttcatttt
     4681 ctgccacatt gcacacataa atcactggct taatggtaag caaaaacagg tcgcttatca
     4741 cctccaggtc gttgtgatcg aggttgaggc tgcgggcgct ttttccttgc tctaaatgcg
     4801 ctttaagggc tgtgagcaca tccatcagtc gcttggcatc cttatcccct gcaccggcag
     4861 cgcgctgtgt tttggtgatg cggacttcta tggtttcgag atctttgagt tggagttcat
     4921 aatcaatgat ttccttgtcg cgtatagggt ctacattgcc atgcacatgc acaatatttg
     4981 gatcgtcgaa gcatcgtacc acgtgaataa tggcatctgt ttcgcggata ttggcgagaa
     5041 atttatttcc caatccctcc cctttcgaag cccctttcac cagtccggct atatcgacaa
     5101 actcaatggt agtaggcact actctttcag gaatcacgag gtcgctgagg gcgttgaggc
     5161 gaggatcggg aacggtaacc acccctacat tcggctcaat ggtgcaaaac ggaaaatttg
     5221 ctgcctgcgc tttagcgctc gacagtgcgt tgaataatgt cgacttaccc acgtttggaa
     5281 gtccaacgat accacatttc aatcccatag ataaattttg gaggtgcaaa ggtacgtgtt
     5341 ttgggggcaa aaagtggagg ggccccgtag gggctgggtt gggatggaga ataggcttga
     5401 tggagagtgg ggctttgagg aaacaaataa ggaagttgga ataatttgga tgggtttgcc
     5461 ccgtaggggc tgggctaaaa tggagcttac ttttcaaccg gagctatccc tggcttgatc
     5521 taaaatgaaa ttctcatcta ttcgatgtgg gtgtgccccg caggggctgg gcttaaattg
     5581 agaatactat tatccggcgc tatccaaaaa acaccctaac cttgaaatcc tactcccaac
     5641 aataaatcta gcccctccgg ggctgcacac tcccaaacta acaccaccct tattttacaa
     5701 tccctacaac acccccaact cctcatagca aaatctccta ctaaatacac tttcttgtgc
     5761 cacccgaact tacccctcct gaaattcaaa tccatattgc tcgtcataaa caattcccga
     5821 ctcatccaac aacttaaaat actcctcctt aaaactccat ttcctatgca gggcatcctg
     5881 ccctataaca taagcaatca tcccctctcg ctccataggt gaacaagata tcccaacata
     5941 ccccgcctgc cacctgaata ccttctcaac ccacccgtta gcattgatat atcccgagga
     6001 atatgccttc aaatctccta tcaacctcga taaattcttt gtcggtggta aggtgatcaa
     6061 tacatgcaca tggtccaagc tgcaatgcac cgcatgcgga atggcttgct ccttatttaa
     6121 aaaactggtc aggtaacggt aaacctgatt attgaactcc tttgtaagta aattatctct
     6181 gccatcaact gcaaatatta catgcaccaa tgtttgtgta aacgtattcg ccatatgctg
     6241 caatgctccg tcatttattt caacagtttg tgatgctaag cgtttatatt cggatataat
     6301 cgcttagggt ggatttggga atgttgcgtt actttaatta ccaacaaaaa aaagccatta
     6361 ctccctgcgg aataatggct cctttaaaaa aaatatatgc ttactgcttc atgatcgaaa
     6421 tcccgaacgc agcttctcct cgtaatacat caatataata tatccccgcc ggtagcgatt
     6481 gcatatcaat aactgtattc ccgacaatct cttgcgtaag caatgcatta cctaatgcat
     6541 cgcgaagtgt gcaaactgcg ggcaatacgt cactgtaaac taccatgata tgatctgtta
     6601 caggatttgg aaataccgat atcgccatac cggaattgcc attttcccca actgcattaa
     6661 aattatctat caccttacat aaagtagctt cacccgctac attacctgct gttaaacata
     6721 cggtgtacga cgtgggcgcc ggatagaaat gcgatggatt ctgctcgctg cttgtaccgc
     6781 catcaccaaa tgaccactcc caatatgctg gtgtattgct gctcaaatca ttgaaattga
     6841 cagtggtgcc tcccggtagc gaccattcga aatctaccac cggaacttta ggtgtgttta
     6901 tgatgatcac agcctgacaa ttggtattaa acccttccgc attggttgcg gtcagacaaa
     6961 caaaatatga gccgtcttct aaaaatgtat gtaccggatt ttgttccgta ctgttagtgc
     7021 catcgttaaa atcccaatac caggaggtag gtaaattcga actcaaatct gtaaatgtta
     7081 ctgtaggatc tcctgtgaaa ctaaatgcag caaccggcac ttcgtaatca ttaatactaa
     7141 ttgcctcaca ataggtatca ctggtgatca tatttgttgc ggtaagacaa acattatata
     7201 ctccattcac taaataagta tgtgtaggat tttcgagcgt cgatgtgcca ccatcgccaa
     7261 aatcccaata ccattctgtc ggcgaacctg tactttcatc tgtaaaattc aatgtagggg
     7321 ttgcgagata atccaccgaa aaatctgcca ttggtgctcc tgttaacgcg gaagtaacta
     7381 cacggaaatg gaagtcgcca aagaactccg accatccgaa agctgtctgc acataacttc
     7441 tgttgatgac accggttcca gtgtacgaaa tccatgccac acctccgggt acatcatatc
     7501 cgataaatac atcgccaaaa atcccttcaa gctcaggcac accgcgcaga tcgatgcgtg
     7561 ttcctttatt cggttcatcc aatgtaggtt caggagttac ataaaatggt gtcacaagat
     7621 ctgcacccgg aaaaccaccg tcatcagccc atacgtggaa tcggatatca ttattcggac
     7681 gcatataatc ggtataattt tgaatgatgg cagttaccac atcagtagaa cctgctaatg
     7741 tcacttttac tgccgccgaa acataacccg taagactgag tgtcccgatg tcggcaacaa
     7801 aatcgattgt ggcatcatcg taaccaatat aatttcctgc aatgatctga taggtttcgg
     7861 ttgttgccgt attaggcaca ccgaaatcgt caattgcctc aatataataa tctatccatg
     7921 aaccgggcgc ctcggtaggt atgatgaaag aataggtatc tccgccgatg aggtcaccgg
     7981 gaactgcagt gaaggcacct ccatcaacat tataatataa agttgcagat gaaattcctg
     8041 aagcatcaat gatctcggca gtgagtgatg tttcctctaa attcccttca tacaaattag
     8101 taggtgtatg caaaatgagc ggtggcgaaa cgtcgatatt atatttcgtg attttaaaat
     8161 catcaatata aagaccgtcg acattatagg cggcatcact ctcgaatcga aaccttacgc
     8221 gcacatcggg actacccaca aaggcaccta tcggtatggt gtaatttgtc catgtaaaca
     8281 tgccttctcc attcagcaca taaacattga tccaggaagc gcctgcatca tctgatacat
     8341 cgacatatac ataatcaaat gccgtttcca gatcaacgag cgccataaat tgcacgtctg
     8401 catccaaggc cgtgctgagg tcggcactca cattcatggc tgcttctgag ctgaacatgt
     8461 cgggataatt tccatccgga ctgtctgtaa aacaggtcgt tggactgtag gcataggccg
     8521 tggtagtgtt ccataatcct gtgtaggtcc agttggcgag accaccctca aaatcatcgg
     8581 aaaaaacgac aacctgagcg cgtgaatgcc actgtacgag gaggatacaa aggagcgagt
     8641 agataatttt tttcatttgt ttagttgttg gatccatcac taaattactc aaaaattata
     8701 agttggcaag ctattcgctg tatagctgtt acctttgcac cgttaacaaa cagccaaatg
     8761 ttaaaacata ctcttttttt cgcctttctg ctcactctgt atatgccatt gcatgcgcaa
     8821 actgccgatc agtggtttgc taccggatac gaaaagtatg aacaacagga ttatgcaggt
     8881 gccatcagcg attataccga atgcattatg ttggcaccgg attttccgga agcatattac
     8941 aatcgcgggc tctgcaatta ttttatgggc tattacgacg cggcgatctt cgattttaat
     9001 caggtgatat tacaaaaacc gggtgaagca cgcgcctatt attggaaagg ttatacgcaa
     9061 tttgctctcg gtcattatga agaagcagag aatgcatatt caaaggcatt gtattacgat
     9121 cctacttatg tagcagcatt ttttgaacgc gcatttacca gatacacgct tggcgatttt
     9181 gctgacgcga tagaagattt taataaagta ttatccatcg atcacagcaa cagcgatgca
     9241 tggtttaatc gcggattgtg ctttattgct atcgctgatt acacttctgc attgcaagat
     9301 ttttcacgcg tagtggaaag caatgctgct gatccggatg cttggtatta cattggattt
     9361 atctacaata aacaattaaa attcgaggaa gctgtaagcg cacttacaaa ggtgataaca
     9421 ctcgaatctg atgccgttga tgctttatat cagcgcggat ttgcatacta caatttattg
     9481 caggatgaag ctgcttgcac cgactggagt gagggcgctt tgttgggaaa tgaactctgt
     9541 aaagaattgt atgaaaaaga atgtatgaaa taattgtggt gtaaattttc tacctctcta
     9601 ttctccttcc atagcacaat acgttgaaat aacggtcggc gcattttcat aacccaggct
     9661 atcggcttta tgcaaataag tacacgcagt tgcctgatcg cccatttgca attttaaaat
     9721 gccatagttg aaatttccca tcggatgatc aggagcatat ttaaataaaa tcaggaagtc
     9781 tgtttcagca tcggtgtatc gccctaacat agcctttgac aatccacgtt gattgtaatt
     9841 ttcaggattg gaaggatcta cctccacagc tgcatcgaaa ctataaatgg catcctcata
     9901 agcaccctgg taatattgcg ccaatccgag attataccat aattgttcat tctcaggatc
     9961 gtacaataat cccacgcgaa atttattttc agcactgata aaatctccgg cattcgcagc
    10021 aaccagtccg tcttcgagat aaaaggctgc catgtcggta ttggcaattg acgatcgctc
    10081 tccgttcatg gtgaaataca cggagtcgtt atccgacgat accgtaattg gctcagtatt
    10141 ctgccctatg acaattgctg tttgcagcac aaaaagtgtt aatacgacga atctcataac
    10201 cataaaacgc attttttact cagtaatggg tacaacagga gcacaaaatc tcctcaaaat
    10261 tgtgtgcgta atccggtagg tcgtctatca tgcaatttaa catatcgcat gaaaaataaa
    10321 tcatcagcaa ttacatgcgt ttgcgttcca accacttgct tttagtggtt tgggtggcga
    10381 tggcactttg ggtttctgct gatttttcgg attccatcaa tcgcgcagct aaaaatgccg
    10441 caatcgccga tgcttcctcg tcgtgaagct gcaatgcttc gggtgtaaaa tatcgttcaa
    10501 taaatttagt atcgaaattc ccactcaaaa atgcctcatg ttgcatcacg aaagtaccaa
    10561 aaggtaaggt agttgcaacg cctgatatct gataatcctg aatggcgcga atcatttttt
    10621 tacatgcgag ttcacgcgtt tgtgcatgca cgattagttt cgccagcaac gggtcataat
    10681 aaataggaat gtccataccc tcttcatatc cgtcgtcaac tcgaatgcca tgtccctgag
    10741 gtcggcgata agtgatgagt ttaccgatat caggaaggaa attatttgta ggatcttctg
    10801 ccgttacgcg caattcaatg gcatgtccat gaatgcgcag atcatcttgt gtgaatgaaa
    10861 gtgctttgcc ttcggcgatg agaatttgtt cgcgcacaag atcaagaccg gtgataaatt
    10921 ctgttaccgg atgctctacc tgcaagcgtg tgttcatttc cagaaaataa taattcaact
    10981 gatcgtcgac aagaaattct actgttcctg cacctgaata attacatgct tttgcaacca
    11041 tcacagcact ttctcccatt gctttccgca tctcgggaga aacaactgcg cttggcgctt
    11101 cttccactaa tttctggtgg cgacgttgaa tagaacattc gcgttcaaat aaatacacca
    11161 cattaccatg atcatcagcc aacacttgaa tttcgatatg tcgcggtgag gtgacaaatt
    11221 tttcgatgaa tactgcatca ttcccaaaag cagagagtgc ttcactcata gcgcgatgca
    11281 tttgttcttc aagctcctct tcattattta caacacgcat tccttttcca ccaccgccgg
    11341 cactcgcctt aattaatatc ggatagcctg cttttttagc aactgctttt gcttccgcaa
    11401 tatcggtaat cgcatgatcg gttcccggaa ccattggaat atgatagttt ttcgctgcct
    11461 gcttagcaga gattttatca cccatcatat ttatgctgta tgcactcgga ccaataaatt
    11521 taattcccgc ttctgcaact ttttctgcga acactgcatt ctcactcaaa aatccatacc
    11581 ccggatgtat cgcatctgct ccgctcattt ttgcaacttc aagaatctta tccatcaata
    11641 gataactctg attcgattgc ggcggaccga taaaatacgc ttcatcggca aatcgggtat
    11701 gtatcgcatt cctatctgcc tctgaataaa cagctactgt tttgatgccc atctctctcg
    11761 ccgtacgcat aactcgcaaa gcaatctctc ccctgttagc cactaatatc ttgttgatct
    11821 tcatagcaac aaaggtggtg aaggagtttt gtttttgcaa gaaggatgga atgtgggaat
    11881 ttgggaatcc cgcttgggcg ggatgtcgct gcgctccatg tgggaattca ttgctacttc
    11941 aatttggctg cacaattgca tatggagtac agtaacagcc cgttcagcga tacttgcaca
    12001 cttgcacacc cgcacacctg cacacttcca cactattaat gggaatgtga ttggttcgcg
    12061 caaagtggca aagctgcaaa gtcgcaaatg ataaatctca ccaacacact caccactcac
    12121 ccactgaccc actgacccac tcacccactc accactcacc cactcaccca ctcacccact
    12181 cctctctcct ctctcctttc tcctctctcc tttatctatc tttgccccat gcagttctgg
    12241 ccctccgttt ggggaatgtt gaaaaaagat ctcatccttg agatgcgtca gaagtatgcc
    12301 atcagcggaa ttctgctgta tatgctctgc atcgttttca tcattttcat gaccttcgga
    12361 cagggcgttc gcggtcccgt ttgggtggta ctgttctggc tgatcatgct gtttacatca
    12421 gtaaatgccg tcgccaaaag ctttttacag gaaagcaccg ggcgtttgct ctattactac
    12481 actattgcct caccccaagc catcatcctg gccaaaatgc tgtacaacat cggtttgatg
    12541 atattgatgt cggcattggc tctcttgatg cacagcatta cgtccggtta tccgattgtt
    12601 gatagtccct cgtttttgct tgccaatgtg ctaggtgcta ttgcttttgc caccaccttc
    12661 agcatgattt ccgccatcgc ctctaaagca tcgaacagtg gcaccatgat ggcgatttta
    12721 agcttcccga tactgattcc aatactgatc atcctcattc gcatttcgct gaacgccata
    12781 caacaaaaca atctggcagt taaccttcag gacctgatgt acttggctgc cttagacgtg
    12841 atgatcctgg ctatggcctt gatcctgttt ccttatctgt ggcgcgattg acatccgcaa
    12901 agcgcttata gattttcaga atactacatt ttttgacctt gtctgacttg gttaaaatgg
    12961 caataccctt gttaattatt acttatcttt gcacccaatt cgcaggctac cccacctgag
    13021 ggtggaaatg aggtaatcaa gatgctcaaa caattgcaca accgctggtg gaagatactt
    13081 tgtgtcgcct ttctggttta cacttttacc gcaggacttc tcattcatac acccgacgtg
    13141 cctttattgc atgaaactgt ccgcaacctg tttttccatg tgccatgctg gttttcaatg
    13201 ctgttattaa tgctgatatc actgatttac agcattaaat ttttgcgttc cggacgcatg
    13261 atacatgatg tgatagcggt ttcactgctt aaagtagcaa tggtgtttgg cgttttagga
    13321 tatctgaccg gtatgctttg gggcaatttt atttggggtg atttgctaag ctggatcact
    13381 aacgacacta agattttagg cgctgcgata gggcttttga tatattttgc ctatttcatc
    13441 ctcagaggat ctattgatga tgaagaaaaa cgtgccagag tagccgccgt atacagcatt
    13501 ttcgcattta tcctgctcaa cgtttccatc aatgtggtac ctcgcctgac cagcagcctg
    13561 caccccggat cgggaggaaa tgccaccttt acggtttacg acctcgacaa taacttgcgc
    13621 atggtattct accctgctgt gtttggatat accttgctgg gattgtggat cagcagtatc
    13681 atcatccgca tgaccattct gcattacaag aaaaacgaca ttccgctcgt atgaaaaaac
    13741 tgacctctct tagccgtttt atagccacat ttcttttttt gctggcaaac attacctttg
    13801 cgggtgctca aaaccaaggt tatgcgacca cagccgatag caacgaaaat ctgctgcgca
    13861 gcaatggtct catttacgtg gttgtaggcg ttatcgttat cattctttca ggcttgattc
    13921 tttacctggt gagcattgac cgcaaactgg gcaagatgga aaaagaatac ggcgataaac
    13981 attgaacatt tttataacca agtattcatt attgtaatta aacttacaaa ctcaataagt
    14041 atgtcaaata ccagtttctt tgaaagcgta gaaaaaaact ttgaaaaagc tgcgcagatc
    14101 acaggtcacc ctcgcggcct cctcgaccaa atcagggtgt gtaacagcgt ttaccacatg
    14161 aaattccccg tgaaaatcga cggcgaattc caggtaattg aagcatggcg cgtgcagcat
    14221 agtcaccacc gcgttcctac caaaggtggt atccgctacg atgccatggt gaatgaggat
    14281 gaagtaatgg cattggcgag tctgatgact tacaaatgtg ccatcgtaga tgtaccattc
    14341 ggtggtgcaa aaggtggtat ccgcatcaat ccacgacagt tctcatctga tcaattggag
    14401 cgcatcacac gtcgctatgc agctgaactt tgcaaaaaga atatgttagg tcccggcctc
    14461 gatgttcctg ctcccgatta cggaacaggt gaacgcgaaa tgagctggat cgtagatacc
    14521 tatatggctt tgaatccggg tagtattgac ggttacggtt gcgttaccgg aaaacctgtt
    14581 acacaacacg gtattaacgg tcgccgcgaa gctacaggcc tcggcgtttt cttcggcgtg
    14641 cgcgaagctt gttcatttgc cgatgatatg gcagaactcg gtttgaaacc gggcctcgaa
    14701 ggaaaaaaag taattgtgca aggtcttggt aacgtaggtt actattctgc gaaattcttc
    14761 caggatggtg gcgctactat cgtaggtatt ggcgaatatg aaggtgctat ttataatgta
    14821 aatggcctcg atgtagatgc agtgttcaat caccgcaaat ctacaggcag catcctcaat
    14881 ttcccgggtg ctacaaacat ggctaatacc gccgaaattc ttgagcaacc ttgcgatatc
    14941 ttaattcctg ctgcattgga aaatgtaatc cacgaaggca atgcgaaaaa gataaaagca
    15001 aaaattatcg gtgaagctgc taacggccct gttaccactg aagctgacga tattctcaat
    15061 ggcatgggtg tgatgattat acctgatatg tatctcaacg ccggtggtgt taccgtgagc
    15121 tacttcgaat ggttgaaaaa cttatcgcac gtgcgtttcg gacgattgac aaaacgtttc
    15181 gaacaaggca gcaacatgcg tattgtagat actattgaag aattgaccgg aaagaaatta
    15241 tcacaaaaag aagtagataa tatcatcaaa ggaccggatg agatcgacct cgttcgctca
    15301 ggtctggaag a
//
LOCUS       DAOQOU010000061        15260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1969_length_15260_cov_5.339888, whole genome shotgun sequence.
ACCESSION   DAOQOU010000061 DAOQOU010000000
VERSION     DAOQOU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15260
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1969_length_15260_cov_5.339888"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLM90_07010"
     CDS             <1..202
                     /locus_tag="PLM90_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89157.1"
                     /translation="PLTTHHSPLTTHHSPLTTHHSPLTKTKNRVTLETKLERYPVMLT
                     NFIYVTIECNLCKSLYYNKLSY"
     gene            complement(196..1002)
                     /locus_tag="PLM90_07015"
     CDS             complement(196..1002)
                     /locus_tag="PLM90_07015"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89158.1"
                     /translation="MKKNAFVWVFGLITISVVGTSMSYQNPNNPPLGYTGAPGEQTCQ
                     KASCHGGGNFVGTPTLSGLPDSIEPGASYSLTLTLESNCTKAGFELTCLDADDVKAGT
                     FSAGTGNSVANFGGKQYVRQSSPKTLTGGSTSWTFTWHAPETAGGDAVTCYFASLNAN
                     GNGNDSGDKAIKGDTTIAYKVPEVILDATEIAKPEITVTPNPATDHISINHAAVGSTM
                     QLFDIHGKLIMSKHINAVSVTYSTAELPAGNYLLAVSNNGERTSKQISIQ"
     gene            complement(1133..2413)
                     /locus_tag="PLM90_07020"
     CDS             complement(1133..2413)
                     /locus_tag="PLM90_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ89159.1"
                     /translation="MVSSLFNNLISLIGVAFMGRVGETELAACGVAALIYITITMIPY
                     GLSVGFQIIIARRAGENNPESIGRIFNNNLIFLMGVAVLLYAFLQFMAPILLKPMFSS
                     PEVYAAGMTYLKYRNIEIFFAVFAFTLLGFYTSVGQNQIISYSAILMLVVNVFFSYNL
                     VFGGMGLPEMGIGGAGLANLISASAACLLNFGYLFVSPLRARFGLFKFDKFDWPIVKK
                     GLELSGPVVLQHMLSTGTWVIFFLMIEKMGPPALAASNVAKEVYMVLGVTTWGVANAT
                     NSLVSNLLGQKRHDEMFDLIKKVVLIALAFSFAFFLFIILFPQVFIGIFTNDPTITAL
                     ATGPVRIVGTCLVMMAVSSVLFRAVTGTGATRYSLLLELITLIVYMVYVIIIIKVLDV
                     NLTIAWTSEFVYWLVLAWLCWTYLKSGAWKRHRV"
     gene            complement(2549..3517)
                     /gene="hemB"
                     /locus_tag="PLM90_07025"
     CDS             complement(2549..3517)
                     /gene="hemB"
                     /locus_tag="PLM90_07025"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HMZ89160.1"
                     /translation="MYSRPRRLRSNAIMREMVAETRFSVQSLMYPMFVTHGKGINHPI
                     GAMPGISHFSPDTLVKEVEHLMSLGINKILLFGVGEDKDPVGSAAYDFTNAVANGVRS
                     LKAAFGNDLLIATDVCMCAYTDHGHCGILHGHEVANDETLTSLAKMALMHAQAGADIV
                     APSDMMDGRVAAIRDTLDAENMQQVAIMSYSVKFASGYYGPFREAADSAPSFGDRKTY
                     QMDVRNSREAIKEALLDVEEGADILMVKPALAYMDLIRTVKDQTLLPLACYNVSGEYS
                     MVKAAAEKGWIDEQRIVMENMASFTRAGADIIITYHAKDMLSKGWV"
     gene            3596..4438
                     /locus_tag="PLM90_07030"
     CDS             3596..4438
                     /locus_tag="PLM90_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HMZ89161.1"
                     /translation="MVHFVDTDFNKDFTSHYSLLFQLEEHQYTYAVYDKRSGKLQLLK
                     TVAFNSAFAPEAFSKFKVSVTAEDALQAMYKEVKIGVVDSAFTLLPRTIFEEEKAKAY
                     LSLTSPIDDQSTVLVNQVRGIFVRNVFSLPLAISSYLDEVFTHPKWFHASTALMETAI
                     RNRDQFADQQIILDIKPGVMYLMYYAKKEFVFMNQFKYVNKDDFLYYVLLMADQFEID
                     REHCDLKLSGEILPDSMLYNELWKFFSNISFLQPNEHIILPDAVREKPVHMFNSLFSL
                     DLCE"
     gene            4429..4986
                     /locus_tag="PLM90_07035"
     CDS             4429..4986
                     /locus_tag="PLM90_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HMZ89162.1"
                     /translation="MRIIAGKFRGRRFHPPANIPARPTTDFAKEGLFNILTNNFNFED
                     VAYLDLFCGTGSLCYEMASRGCTDIVGVEMDNRSVDFIRKTSTELNITINVLRMDVFE
                     YMRTTKKKFDLIFAGPPYPLPNIPELPEEVFANQLLNPEGWFVLETNPKYSFTDHPHF
                     LRSRNYGTTIFHIFQAEVVKRGDAV"
     gene            5002..5466
                     /gene="coaD"
                     /locus_tag="PLM90_07040"
     CDS             5002..5466
                     /gene="coaD"
                     /locus_tag="PLM90_07040"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMZ89163.1"
                     /translation="MTKIAVFPGSFDPVTLGHINIIERALPLFDQIIIGIGHNSTKSG
                     FFSLEQRVNWLQEIFLHEKKVQIHAYEGLTIDFCRHNHAQFLLRGLRSTADYNYELAI
                     AQTNKSLAPEIETYFLMSDPAHAHINSSIVREVYKNGGDISSMVPQIVISGN"
     gene            complement(5463..6110)
                     /locus_tag="PLM90_07045"
     CDS             complement(5463..6110)
                     /locus_tag="PLM90_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ89164.1"
                     /translation="MPQSYKIFINENPVFLIAGSIPVNANLNDSAHPILNFNQEKEVE
                     KAFHLLENHAGIKSLTIYGDNAKEMRNTLFAGYKKIAAAGGLVFNNKGQVLLIYRRNS
                     WDLPKGKIDAGEKKKAAALREVREETGLEKLSIIRKLKKTYHTYLLENNTPVLKVTHW
                     YLMMCNDVTPPVPQTEEDIELATWIDIDEVELKFNKTYANIQDVIHSGVTMMHYG"
     gene            6109..6750
                     /gene="pyrE"
                     /locus_tag="PLM90_07050"
     CDS             6109..6750
                     /gene="pyrE"
                     /locus_tag="PLM90_07050"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMZ89165.1"
                     /translation="MLFQSDDAIVFAEKLLAIEAIKLRPNEPFTWASGWRSPIYCDNR
                     LTLSYPEVRTWIKNTLAKGIRQQFPNVEVIAGVATAGIPHAALVADAMGLPMIYVRSE
                     AKKHGLSNQIEGRCAPGSRVVVIEDLVSTGGSSLTAVAALRNVEAQVLGMAALFTYAF
                     PVADEAFNAASCKLFTLSDYPSLITHAVKNQIIPADTADMLNTWRESPSTWMQ"
     gene            6738..7253
                     /locus_tag="PLM90_07055"
     CDS             6738..7253
                     /locus_tag="PLM90_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89166.1"
                     /translation="MDAVTAHIVSGAAAGFVGGCVNIYLWQHTLRHKKLLGVKLHKGF
                     AAVVADILQQIPEKFDTKNLKHIEKDILDLFDHFMSDQLTKKMPVLSMFMDEKLIAEI
                     RTIFKLEMESHLPKLLMNRITAEKNITMVAGLVAKAVRKGLRQYAKHAIIYILLGTLG
                     GALVGYAMSFI"
     gene            complement(7302..9803)
                     /locus_tag="PLM90_07060"
     CDS             complement(7302..9803)
                     /locus_tag="PLM90_07060"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ89167.1"
                     /translation="MIRQLAITLLAGAVIYTASAQPCIPTYTAGTTDNDYIESFECNT
                     ISNPGSGPGDGSGYTDFTDLSTDLVINETYEIFVENTPYFGENYRIWIDYNHDNIFSS
                     TEEIVTLFSLAAGGSTTLSFTVAMTASPGETTMRIRCVYGTTDFDACSGETYGETEDY
                     AVNIIGLANDVGVSIVNDITDDCDLSSDETVSIIVTNYGTEPASGFSVSYFVDGGTVN
                     TEMFPGGILPGASSPFTFSAGADLSSDGTHVITAWTNYADDMFNGNDSSDVSINNLFT
                     YLTTGFPENICYDGGTIFPSPLAGGGTWSGEAIIDPLTGEMDPTLVGGIGGSTDVTYS
                     FTTTGSYTVTQIPYAPAVPAFETTLGLGDDATANDINIGFPFTYFGNTYTKLFISSNG
                     LIGFSAPSTSYTQQHFPNAADPDNIIAFCWTDLNPSAGGSISYETIGVAPNRKFIVYY
                     DDVVHYLGTATVTGQIVLHETSSAIDIIAVNIESDGGNMTQGIENTNGTEAYNAGEAY
                     NNAVWTMSEMSWRYAVTPCDGEVTETINFVTPPVVTLPDSAVCQGSSVTLDAGTGAEY
                     YVWSTGENTQSITVDETDDYWVTYYANEFCFVTDSASIIVNSNPPVDLGPDGTKCEGT
                     MLDAENPGAEYLWNTGATTQTLFVTEPGTYSVYVENPITGCYNSDTITMSIIPLPIAD
                     YSAVATDLTVVFTSLAGDVDTWFWDFGDGSTSIEENPWHIYPFAGDYTVTFVVTNGCG
                     ADLISEVFSITTGINALSQNALSIYPNPASTTIHVSGLPNGQEQHFRIMNMTGSVVLE
                     GKSMHDIDISALPASTYVLLVDDGSGQFKGTFVKD"
     gene            complement(9830..10207)
                     /locus_tag="PLM90_07065"
     CDS             complement(9830..10207)
                     /locus_tag="PLM90_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89168.1"
                     /translation="MKKLVTLLFVAVSTISITFAGNQGPVTATLDEHMKIVLPTDQPM
                     AQEYVIDIAGRFVSAEVLNSFCDSFRDQGVSYRGDFAAGKMYISVTPLTDSTNHTWDA
                     AKWNEYFSSRSFKMEYFMQSMNK"
     gene            complement(10286..10723)
                     /locus_tag="PLM90_07070"
     CDS             complement(10286..10723)
                     /locus_tag="PLM90_07070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ89169.1"
                     /translation="MISDHFLRLEQLFLKAPIQQNIIGQTISVAEGTALIKYHVNNAF
                     FHGGNAQHGAIYFKLLDDASYFAAASLVSDRFLLTASFEIKLFKPVTEGMLTATGSCK
                     MAGDRIIYAEANLMNEHQELVATGAGRFQRGKQLWASLPGYSG"
     gene            complement(10720..12891)
                     /locus_tag="PLM90_07075"
     CDS             complement(10720..12891)
                     /locus_tag="PLM90_07075"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89170.1"
                     /translation="MIRKLSFTIVLFCSVVVLSAQFKPNTTITASQYGSNLDLAFMGG
                     VTAPQFSDIDVNQDGLMDLFIFDRGAWRPFILLRDISGDYTYAPQYEKIFPHMEDWAL
                     IRDYNCDDVPDIFTYSIGSTAVYKGSFDGGSLHFTLVDDDLTYVDGTGTTSLYTSRTD
                     IPGIADIDQDGDIDVLSFSVSNATIRFYKNISAEQGYGCDSLIYDLVEYCWGEIFEEA
                     SCAGATMNVACKGGGDMSAASEAKIELHIGSTIVTYDTDGDGDHDAMIGDNLCNNIVF
                     FRNGGTTDFAQMSSKDTAFSSTNVSFDLALFPAAYLSDVDDDGDEDMLITTNDHLIGA
                     NMQNVWLYENLNTNDTFDLVYATDTFLVSEFIDAGIYSKPTFFDYNNDDLMDILVGVG
                     STLGKDDVLKHGLWLYENVGTPTSPSFELVSTDYAGLNAYPISQLSPVAGDIDNDGDD
                     DLLIGINDGTICYLQNIGGPTGEAIFTTAVFVYQGIDVGQLASPCLIDIDEDGKLDLV
                     IGEQNGNLNYYHNTGTATSPVFTSESEIWGGVDVRKPGFITGYSSPFMYRNENDSLYL
                     LVGSQSGFVYAFNEIEDALLGEFYEKDTNYLKWDPGVYASIYGQDITGDGEMEFLVGT
                     IRGGIQIFERDKGTAIAEISNEANLQLFPNPSDGNFTITWDQESAALVEVYTMMGNII
                     WSESVYGSALQVNLPESIAKGVYMIRVSYPGARGTLQYIKQ"
     gene            complement(12888..15062)
                     /locus_tag="PLM90_07080"
     CDS             complement(12888..15062)
                     /locus_tag="PLM90_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89171.1"
                     /translation="MLKKLLFPLAVSILVYSSAVAQHYMYNYNVPVLQGTTNYQNPFA
                     GGLNNPQFSSIDINQDGKKDLFLFDRSGNIPMIFINRGGTGEINYEYRAEYASRFPAM
                     RDWAILLDYNCDGLEDIICGYDNGIKTYLAASDGGSINFTEDASKLQFTEAGFFFDLP
                     VGYIDIPGFADIDQDGDIDVITFNMIGGIVDHYDNQAVEDGLPCGTWALEHINSCWGN
                     FYESGITYGVDLNYDCKGIVRTADGLHAGSTFMIFDEDADNDMDVVLGDLAFDVLNKL
                     TNGGDSELAYITDQDTTFPSYDKPYDLPAFPAPFLIDINNDGAKDMVVAPNNINSSEN
                     FKNVWYYQNISTDDTYVFDYRQDTLMVSTMLDFGDGAFPVFFDYNYDGLLDLIVGNYE
                     YFDDGDQIGKIALLQNTGTADAPAYTLITRNLGNISDFGFHGIAPTFGDLDGDGDQDM
                     LIGEEEGFLHLFKNIGPPEGPAEFIFFTGNYQGIDKGQNATPQLFDVNEDGLLDLILG
                     ERNGNINYFENTGTATTPVFTLVSEFWGNVDVRSTGVLTGYSAPCMYRSPEGDMTLYV
                     GCEEGTIFQYQPAADITAPFIEVTDHFSDIDCGAFSSVNINEINNDDYPDIICGNKRG
                     GVTLFRDDRSTGIANHEAVSPLIFPNPSTDNIYIDGLQKITGPILISVCSIDGKEVKS
                     TMVSSGNMPVQLSLSDIASGVYVLHITTRQEANISSQLLIKQ"
     gene            15076..>15260
                     /locus_tag="PLM90_07085"
     CDS             15076..>15260
                     /locus_tag="PLM90_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89172.1"
                     /translation="MIKIQQACCFVASISVFDTAVIGVCKVWHGILFMDSNMLLHHNK
                     KASYESEKCATYRRRRGL"
BASE COUNT         4501 a         3371 c         3062 g         4326 t
ORIGIN      
        1 accactcacc actcaccact caccactcac cactcaccac tcaccactca ccactcacca
       61 ctcaccactc accaaaacaa aaaaccgggt aacactcgaa actaaacttg agcgttaccc
      121 ggtaatgtta accaacttta tctacgtgac tatagaatgt aatctatgta aatcattata
      181 ttacaataag ttaagttatt gaattgaaat ttgtttgctg gttcgttcgc cgttgttgct
      241 aactgcaagc aggtaattcc cggcaggcag ttcagcagtt gaataggtta cagaaactgc
      301 gttgatatgc ttagacatga tcagctttcc gtgtatatca aacaattgca tagtagaccc
      361 aacagccgca tggttgatgc tgatatggtc ggtagcagga tttggcgtga ctgtgatttc
      421 aggtttagca atctcggtgg catccagaat cacttcagga actttatatg caatggtggt
      481 atcacccttg attgctttat caccgctatc gttaccattg ccgtttgcat ttaatgaagc
      541 aaaatagcag gtaactgcat caccacccgc tgtttcagga gcatgccaag tgaaggtcca
      601 ggatgtgctt ccgcccgtaa gcgtttttgg tgaactctgg cgaacatatt gtttaccacc
      661 gaaattcgct acggaattcc cggttcctgc cgaaaacgtt cctgctttca catcatcggc
      721 atccaaacag gtgagctcaa agcccgcttt ggtgcaatta ctctccaatg tgagcgttaa
      781 tgaataactt gctcccggtt caatagaatc aggtaaacca cttaaggttg gcgtccctac
      841 aaagttgccc ccaccatgac aacttgcctt ctgacaagtc tgttctccgg gcgctcccgt
      901 gtaacctagt ggtgggttat tgggattttg ataagacatg gaggtgccta ccacgctaat
      961 agtaatgagt ccgaaaaccc atacaaatgc attctttttc attaatttat aaattattaa
     1021 ttatgaactg cgctgtgcag cgtggagagt gagtcaaagg taggaaatat gtatatacat
     1081 acaatataca gtaaggtatt gtcttgaaac ggtgatgagt ttaacatttg gattaaaccc
     1141 tgtggcgctt ccatgcacca cttttgagat acgtccagca taaccaggcc aatacaagcc
     1201 aatacacaaa ctcgcttgtc caggcaatgg tgagattgac atccaatact ttgataataa
     1261 tgatcacata aaccatatat acgatcagcg tgatcagctc cagcaacaag gaatatcttg
     1321 tagctccggt gcctgttacg gctctaaaca atacagatga aacagccatc attaccagac
     1381 aggtgcctac aatgcgcaca gggcccgttg ccagagcagt aatggttggg tcgttggtga
     1441 aaataccgat gaaaacctgc gggaacagaa taataaacag aaagaatgca aaggagaaag
     1501 caagtgcaat caatacgacc tttttaatga gatcgaacat ctcatcatgt cgtttttgtc
     1561 ccagaaggtt actcacaagg ctatttgtgg cattggcaac accccaggtg gttacaccca
     1621 acaccatata tacctccttc gcaacattgg atgccgccag ggcaggaggt cccatttttt
     1681 ctatcatgag gaaaaatatt acccaggtgc ccgtagagag catgtgctga agtacaacag
     1741 gaccgctgag ttcaagcccc ttcttaacaa tgggccagtc gaatttatcg aatttgaaca
     1801 aaccaaaccg cgctcttaaa ggactcacaa acaagtaacc gaaattgagc aaacatgccg
     1861 cagaggccga aatgaggttg gcaagtcctg ctccacctat acccatttcc ggcaagccca
     1921 ttccgccaaa aaccaggttg tagctgaaga aaacattcac caccaacatt aaaatcgcac
     1981 tgtaagagat gatttgattt tgtcctacgc tggtataaaa ccccagcaag gtgaaagcaa
     2041 atacagcaaa aaatatctca atattcctgt atttcagata agtcatgcct gccgcataaa
     2101 cctccggaga actgaacatc ggcttaagca agatgggcgc catgaactgc aggaatgcgt
     2161 ataaaagcac cgccacaccc atcaggaata tcagattatt gttgaaaatg cgaccgatag
     2221 actcgggatt gttttcgccc gcccgcctgg caataatgat ttgaaaccca accgacaacc
     2281 catagggtat catggttatg gtgatgtaga tcaaagcagc tacaccacaa gctgcaagct
     2341 cagtttcgcc tactcgcccc ataaatgcaa cgccaatcag gctgatcagg ttgttgaaca
     2401 ggctgctcac catgaggggg atactcaggc gtataatttc tcggtatgtg atattaattt
     2461 gcaatccgta aaatgcggag gtaaagatgc actaatcttc aggcttttcc atcggcggag
     2521 aagacgaact atacatagga attgtggatt acacccagcc tttggacagc atgtcctttg
     2581 cgtgataagt aataatgata tcagctccgg cacgagtaaa ggaagccata ttttccatta
     2641 ctatccgttg ttcatcaatc cagccctttt ctgctgcggc ttttaccatg ctgtattctc
     2701 ctgaaacatt ataacatgcc agcgggagca aggtttggtc tttcacagtg cgaatcagat
     2761 ccatataggc cagagccggt tttaccataa gaatatcggc accttcctct acatccaata
     2821 aagcttcctt gatagcctct ctgctatttc ttacatccat ttgataggtc ttgcgatcgc
     2881 cgaaagatgg ggctgaatcg gctgcttcgc gaaaaggtcc ataataaccg gaagcaaact
     2941 tgactgaata gctcataata gccacctgtt gcatgttttc ggcatcaagt gtatctcgaa
     3001 tggcagcaac tctgccatcc atcatatcgc ttggcgcaac aatatcagcg cctgcttgtg
     3061 catgcatcaa agccattttt gcaagcgatg tcaatgtttc atcgtttgca acctcatgtc
     3121 cgtgaagtat cccacagtgt ccgtgatcgg tatatgcgca catgcatacg tctgtggcaa
     3181 tgagcagatc atttccgaat gccgccttca atgaccgcac gccattcgct acggcgttgg
     3241 taaagtcgta agctgcgctg ccaactggat ctttgtcttc ccccacaccg aataacagta
     3301 tcttattgat acccaaactc attaagtgct ccacttcttt taccagagta tcgggcgaaa
     3361 agtgactgat gcccggcatt gcacctatcg ggtggttgat gcctttgcca tgtgtgacaa
     3421 acatcggata catcaacgac tgcaccgaaa agcgtgtttc ggccaccatt tcacgcatga
     3481 tggcatttga acggagtctg cgcggacggc tatacatctt gagatttttt gcaaaggtat
     3541 catcatacag gcgataaaac gacacccggt aaaaaaacag taattttacc cgcttatggt
     3601 gcattttgtt gacacggatt ttaacaagga ttttacgagt cattactcac tgttatttca
     3661 attggaagag catcaataca cctatgctgt ttacgataaa cgcagcggta agctccagtt
     3721 attgaaaaca gtagcattta attcagcatt cgcaccggag gcgttttcaa aatttaaagt
     3781 gagtgtcact gcagaagatg cccttcaggc aatgtataag gaggtgaaaa ttggtgttgt
     3841 agacagtgct ttcaccttat taccaagaac aatctttgaa gaggaaaaag caaaggcata
     3901 cttgtcgctc acttctccca tcgatgacca atctactgtt ttagtaaatc aggtaagagg
     3961 aatattcgta cgcaatgtgt tcagcttgcc gcttgcaatt tcctcctatt tagacgaagt
     4021 ttttacgcat ccaaagtggt ttcatgccag tactgcattg atggaaactg ctattcgcaa
     4081 tcgcgaccag tttgccgatc agcagatcat ccttgacata aaaccgggtg taatgtatct
     4141 gatgtattat gccaaaaagg aattcgtgtt catgaaccaa tttaagtatg tgaacaagga
     4201 tgatttcttg tattacgtat tattaatggc cgatcagttt gaaatagatc gcgaacactg
     4261 tgatttgaaa ctgtcgggcg aaatattacc tgattccatg ttgtataatg aattatggaa
     4321 attcttcagc aatatttctt tcttgcagcc gaatgaacac attattttac cggatgccgt
     4381 gagagaaaaa ccggtgcata tgtttaattc actcttcagc ttagacctat gcgaataatt
     4441 gccggaaaat ttcgtggaag aagatttcat ccgccagcca atattccggc caggcctaca
     4501 acagattttg cgaaagaggg attattcaat atcctcacca ataattttaa tttcgaagat
     4561 gttgcctatc tggatctgtt ctgtggaacg ggtagcctgt gttatgaaat ggcttcgcgc
     4621 ggttgcacgg atatcgtagg cgtggaaatg gataatcgca gtgtcgattt cattcgcaaa
     4681 acatcaacgg aactgaacat taccatcaat gtgcttcgaa tggatgtgtt tgaatacatg
     4741 cgcacaacca aaaagaaatt cgatttgata tttgcaggcc cgccatatcc gctacctaat
     4801 atcccggaac tgcctgaaga ggtttttgcc aatcaattat taaatcctga gggatggttc
     4861 gttttagaaa caaatcctaa atattcattt acagatcatc cacatttttt gcgtagtcgc
     4921 aactatggaa ctaccatatt tcatatcttt caggcggaag ttgtgaagcg tggagatgcc
     4981 gtttaaaaca ttgtatttgt tatgaccaag attgccgttt ttcccggttc atttgaccct
     5041 gttaccttag ggcatatcaa tattatagaa cgcgccttgc cgctatttga tcagattatc
     5101 attggcatcg ggcacaacag cacaaaaagc ggatttttta gtctcgagca acgtgtaaac
     5161 tggctgcagg aaatatttct gcacgagaaa aaagtgcaga ttcatgctta tgaaggactc
     5221 acaatcgatt tttgtcggca caatcacgct caattcctgc ttcgcggctt aaggtccacc
     5281 gcggattaca actatgagtt ggctattgct caaacaaaca aatcattggc gccggaaatt
     5341 gaaacctact ttttaatgag cgatccggcc catgcgcaca ttaattcgag tatcgtaagg
     5401 gaagtgtata aaaacggtgg cgatatcagt tctatggtgc cacaaattgt aatttcgggt
     5461 aattaaccgt agtgcatcat cgttacacca ctatgtatca cgtcttgaat attagcgtag
     5521 gttttgttga actttaattc cacctcatcg atatcgatcc atgttgctaa ttcgatatct
     5581 tcttcagttt gcggaaccgg cggcgttaca tcattgcaca tcattaaata ccaatgtgta
     5641 acctttaaaa caggtgtatt attttccagt aaataggtgt gataagtctt ttttagtttg
     5701 cggatgatgg atagtttctc tagtccggtt tcctcgcgca cttcgcgtaa agcagcggcc
     5761 tttttctttt cgccggcatc tattttgccc ttaggcaaat cccaactgtt gcgacgataa
     5821 atcaataata cctgtccttt attattaaag accaatccac ctgcggctgc aattttttta
     5881 taacctgcaa acaaggtgtt tcgcatttct tttgcgttgt caccataaat agtcaggctt
     5941 ttaattccgg catgattctc gagcaggtga aaagcttttt caacctcttt ttcctgatta
     6001 aaattcaata tcggatgcgc gctgtcgttg aggttggcat ttacaggaat tgatcctgca
     6061 atgaggaaaa caggattttc gttgatgaaa attttatagc tttgaggcat gctctttcaa
     6121 tctgatgatg cgattgtttt cgcggaaaaa ttacttgcta tcgaggccat caaactgcgt
     6181 ccaaatgaac ccttcacctg ggccagcgga tggcgctcac cgatctattg cgacaacagg
     6241 cttaccttat cgtatcccga agtcagaacc tggatcaaaa atacgttagc aaaaggtatc
     6301 aggcaacaat ttcctaacgt tgaagtgata gcaggggtgg ctaccgccgg aattccacat
     6361 gcagcgttgg ttgcagatgc catgggttta cctatgattt atgtgcgctc cgaagctaaa
     6421 aagcacggac tatcaaatca aatagaaggc cgatgtgcac cgggaagcag ggtagtggtg
     6481 attgaagacc tggtgagcac cgggggaagt agtttaacag cagttgcagc ccttcgcaat
     6541 gtcgaagcgc aggtgcttgg aatggctgct ctcttcacct atgcatttcc ggtagcggat
     6601 gaggctttta atgctgcttc ctgcaaattg tttacactgt cagattatcc ttctttaatt
     6661 acgcatgccg tgaaaaatca aataattccc gccgatacag cagacatgct taatacctgg
     6721 cgcgaaagcc cttctacatg gatgcagtaa ctgcacatat cgtttccggt gccgctgccg
     6781 gctttgttgg tggatgtgtg aatatctatt tatggcaaca cacccttcga cataaaaaat
     6841 tgctgggtgt aaaattgcat aagggctttg ccgcagtcgt tgctgatatc ctacaacaga
     6901 ttcccgagaa atttgacacc aaaaatctca aacatatcga aaaagatatt ctcgatttgt
     6961 ttgatcattt tatgtcggat caactcacaa aaaaaatgcc ggtgctgagt atgttcatgg
     7021 atgaaaaatt aattgcggaa atacgaacca tttttaaatt ggaaatggaa tcgcatctcc
     7081 ctaaattatt gatgaaccgt atcacagcag aaaaaaacat caccatggtt gccggtttgg
     7141 tggcaaaagc agtgagaaaa ggattgcgac aatatgctaa acatgctatc atctacatcc
     7201 tgcttggaac attaggcggt gcattagtag gatatgcgat gagctttatc taaacaatgc
     7261 aatatcaaca ttaactacaa tctgctgctt ccggtaagca attagtcttt cacaaaggta
     7321 cccttaaatt gcccggatcc atcatccact agcaacacat aagttgatgc aggtaaggca
     7381 ctaatgtcga tatcatgcat tgactttcct tcaagcacta cgcttcctgt catattcata
     7441 attctgaaat gttgctcttg accattgggt aatccgctta cgtgaatggt tgtagaagcc
     7501 ggattaggat aaattgataa tgcattttga cttagggcat taataccggt tgtaatactg
     7561 aacacctcag atatgagatc tgcaccgcaa ccattcgtaa caacaaaagt aacggtataa
     7621 tcacccgcaa acggatatat atgccaaggg ttttcttcaa ttgaagtact tccatcacca
     7681 aaatcccaga accatgtgtc aacatcgccg gcaagacttg tgaaaactac ggtaaggtca
     7741 gttgctactg cagagtaatc tgcaataggc aatggaatta tactcatggt gattgtatcg
     7801 ctgttataac atccggtgat aggattttct acatacactg aataggtgcc gggctcagtg
     7861 acaaaaagcg tttgtgtggt agcaccggta ttccacaaat attctgcacc tggattttct
     7921 gcatcaagca tggtgccttc gcattttgtt ccatcggggc caagatctac aggaggatta
     7981 ctgtttacaa taatacttgc tgaatcagtt acaaagcaaa attcattggc ataatatgtc
     8041 acccaataat catccgtttc atcaacagta atggattgtg tattttcccc tgtactccat
     8101 acataatact ccgctccggt gccggcgtca agcgttacgg aagagccctg gcaaacagca
     8161 ctatcgggta atgtaacaac aggtggtgta acaaaattga ttgtttcagt aacctcccca
     8221 tcgcaaggcg taacagcata tcgccaagac atttcggaca ttgtccaaac cgcattatta
     8281 taagcttcac cggcattata tgcttcagtt ccatttgtgt tttctatgcc ttgcgtcata
     8341 ttaccgccat cactttcaat atttaccgcg atgatatcga ttgcacttga tgtttcgtgt
     8401 aaaacaatct gacctgtaac agtggctgtg ccaaggtaat gcacaacatc gtcataataa
     8461 acgatgaatt ttctgttcgg agcaacacca attgtttcat atgaaattga tccgccggca
     8521 gatgggttta aatctgtcca gcaaaatgca ataatattat caggatcggc tgcattagga
     8581 aaatgttgtt gggtataaga agttgatggt gcactaaaac caattagtcc gtttgacgag
     8641 ataaataatt tcgtgtaagt attgccgaaa taggtaaatg gaaatccgat attaatatca
     8701 ttggcagtag catcatcacc aagtccgagt gttgtttcaa atgcaggaac tgcaggagca
     8761 taaggtattt gtgtaacggt atagctacct gttgtggtaa aggagtatgt gacatctgtg
     8821 cttccgccaa ttccaccaac taaagttgga tccatttcgc cggttaatgg atcgataatg
     8881 gcttcaccac tccatgtgcc gccgcctgca agaggggagg ggaatatggt tccgccgtca
     8941 taacagatgt tttcaggaaa tcctgtggtt aaataggtaa acaaattatt tattgataca
     9001 tcactggaat cattgccgtt aaacatatca tcggcataat tggtccatgc tgtgatcaca
     9061 tgtgtgccat cgctgcttaa atcagcgccc gcactaaaag tgaatggtga agaggccccg
     9121 ggtaaaatac cgccgggaaa catttctgtg ttcacagtgc caccatcaac aaaatagctc
     9181 accgaaaaac ctgatgcagg ttccgtacca taattagtta cgataatact tactgtttca
     9241 tcggatgata aatcgcaatc atcggtaata tcatttacaa ttgatacgcc tacatcgttt
     9301 gctaatccaa taatgtttac agcataatct tcagtttccc catatgtttc accactgcag
     9361 gcatcaaagt cggtagtccc gtaaacacag cgaatacgca tggtggtttc accgggtgat
     9421 gcggtcatcg caacggtaaa actcagtgta gttgaacctc cggcggctaa agaaaaaaga
     9481 gtaacaatct cttcggttga ggaaaatata ttgtcgtgat tatagtctat ccaaatgcgg
     9541 taattctcgc cgaaataagg ggtattctct acaaagattt cataagtctc attaattaca
     9601 agatcagtac ttaaatctgt gaaatccgta tatcctgaac catcaccggg gccgctgcct
     9661 ggattcgaaa tggtattaca ttcaaaactt tcaatataat cattgtcggt agtaccggca
     9721 gtgtaagtgg gaatacaagg ctgtgctgaa gcagtataaa taactgcacc ggcaagcaac
     9781 gtaattgcaa gttgcctgat cacgtgtgtt gaggtttaat aatgaactat tatttattca
     9841 tgctctgcat gaagtattcc atcttaaagg aacgagagga gaaatattca ttccatttag
     9901 cggcatccca tgtatgatta gtgctgtcgg taagcggtgt aacgctgata tacattttac
     9961 ctgcagcaaa atcaccgcga taactcactc cctgatcgcg gaagctgtcg cagaaagagt
    10021 tcaatacttc tgcgctaaca aatcgccctg caatgtcgat tacatattcc tgcgccatag
    10081 gctgatctgt aggcaacaca attttcatgt gttcgtcgag cgtagcagta acaggacctt
    10141 gattgccggc aaatgttatg gaaatagtgc tcacagcaac aaaaagaaga gtaacgagtt
    10201 ttttcatatc agtgacttta cagcttaaag ataccaatct gccagtaaca tgcaaagtcc
    10261 ggaggtaagg atacctgaga gatagtcatc cgctatagcc cggcagtgaa gcccagagct
    10321 gcttccccct ttgaaagcgg cctgcacctg tggctacaag ctcctgatgt tcattcatca
    10381 aattggcttc cgcataaatg attcggtcac ctgccatttt acagctcccg gtagcagtca
    10441 gcatgccctc tgtaaccggc ttgaaaagct ttatttcaaa cgacgcagtt aacaaaaagc
    10501 ggtccgaaac caatgaggct gcagcaaaat aggaggcgtc gtccagcagt ttgaagtata
    10561 tggctccatg ttgagcgttc cctccatgga aaaaggcatt gttaacgtgg tatttgatca
    10621 aagcagttcc ttctgctacc gaaatcgtct gcccgataat gttttgttgt atcggggctt
    10681 tgagaaatag ttgctccagt cttagaaaat gatcgctgat cattgcttaa tgtattgcag
    10741 tgttcctctt gcgcctggat aagaaactct tatcatatat acacccttag caatggattc
    10801 cggcaagttt acttggagag cacttccata aacgctttct gaccaaatga tattacccat
    10861 catcgtatac acttcaacaa gcgcggctga ttcctgatcc catgttatgg tgaaattacc
    10921 atctgaagga ttcgggaata attgcaaatt cgcttcattg ctgatctctg cgattgcagt
    10981 tcccttatct cgttcaaaaa tctgtatgcc acctcgaata gtgccaacaa gaaattccat
    11041 ctcgccgtca ccggtgatat cctgtccgta aattgatgca taaacacccg gatcccattt
    11101 caaatagtta gtgtctttct cataaaattc acctagtaat gcatcttcaa tttcattgaa
    11161 cgcataaacg aatccgcttt gtgaaccaac taacaaatac aaactatcat tttcattccg
    11221 atacatgaag ggagagctgt accctgtaat aaatccaggt ttccgaacat caacaccacc
    11281 ccaaatctca ctttcacttg taaaaaccgg tgatgttgcc gtgccggtat tgtgatagta
    11341 atttaaattg ccattttgtt ctccaatcac taaatcaagt ttgccatcct catcaatatc
    11401 aatcaaacag ggacttgcaa gctgaccgac atcgataccc tgatatacaa aaactgcagt
    11461 tgtaaatata gcctcaccgg tcgggcctcc aatattttga agatagcaga tggtaccgtc
    11521 attaatacct attaataaat cgtcgtcgcc atcattgtca atatcgcctg ccaccggact
    11581 gagctgactt atcggatagg catttaatcc tgcataatcg gttgaaacaa gctcaaacga
    11641 tggtgatgtt ggtgtgccta cattctcata aagccaaagt ccgtgtttta aaacatcatc
    11701 tttacctaag gtacttccaa cgccaaccaa aatatccatc aggtcatcgt tattataatc
    11761 gaaaaaagta ggcttgctgt agattcctgc atctatgaat tcacttacta aaaaagtgtc
    11821 agtcgcgtat acaaggtcga aggtgtcgtt tgtattcaga ttttcatata accagacatt
    11881 ttgcatgtta gcgcctatca aatgatcatt agtggttatc agcatatcct cgtcgccgtc
    11941 atcatcaaca tctgataaat aggcagcagg aaataatgct aaatcaaagg agacattggt
    12001 gctggagaat gcagtatcct tgctcgacat ttgggcaaaa tctgtggtgc ctccatttct
    12061 gaagaacacg atattattgc ataaattatc gccaatcatg gcatcgtgat cgccgtcgcc
    12121 gtcggtatca taggttacaa tggtagagcc aatatgcaat tcaattttcg cctctgaggc
    12181 tgcgctcata tcacctccgc ctttgcatgc cacattcatg gttgcgcctg cgcatgatgc
    12241 ctcttcaaaa atttctcccc aacaatattc tacaagatcg taaataagtg aatcgcaacc
    12301 atatccttgc tctgctgaaa tatttttata aaaacgtatg gttgcattgg aaacgctgaa
    12361 cgacaacaca tctatatcac catcttgatc aatgtcggca atacccggaa tgtctgttct
    12421 gctggtgtat aatgatgttg tgcctgttcc atcaacatag gtaagatcat catcaaccaa
    12481 tgtaaaatgt aacgagccac catcgaaact tcctttgtaa acagcagtgc tgccgataga
    12541 ataggtgaaa atatccggaa catcatcgca attgtaatcg cgaatcaatg cccaatcttc
    12601 catatgcgga aatatctttt catattgagg tgcgtaagta taatcacctg aaatatcgcg
    12661 taataaaatg aatggtcgcc atgcacctct gtcaaaaata aacaggtcca tcaacccatc
    12721 ctgattcaca tctatatctg aaaattgcgg agcggtaaca cctcccataa acgccagatc
    12781 aaggttgctg ccatattgag atgccgtaat agtggtattc ggtttgaact gcgctgataa
    12841 tactacaaca gagcaaaata aaacaatggt gaaagatagt tttcttatca ttgtttgatc
    12901 aataattgtg aagaaatatt tgcttcctgt cttgtagtta tatgcaatac ataaacccca
    12961 cttgcaatat cactgagcga taattgaacc ggcatattac cggatgaaac catcgttgat
    13021 ttcacttcct taccgtcgat actgcaaaca ctaattaata tcggaccggt tattttttgc
    13081 aatccatcaa tgtaaatatt atcagtagac gggttgggga atattaaagg agaaacagct
    13141 tcatgattag caataccggt tgatctgtca tctctgaaaa gtgtaacgcc tccgcgtttg
    13201 ttaccacaaa tgatatccgg gtaatcatca ttatttattt cattgatatt gacactgctg
    13261 aaagcgccac aatctatatc gctgaaatga tcggtaactt caataaatgg agctgtgata
    13321 tctgctgcag gttgatattg gaatatggta ccttcttcgc atccaacata caaggtcata
    13381 tcgccttcag gagaacgata catacatggc gctgaatagc cggttaacac gcctgtactg
    13441 cgcacatcca catttcccca aaattcactt accagagtaa aaacgggtgt ggttgcagtg
    13501 cctgtatttt caaaatagtt aatattcccg ttgcgctcac ctaaaataag atcgagcaat
    13561 ccatcttcat ttacgtcaaa caattgtggt gtggcatttt gccctttatc tataccttga
    13621 taattacctg tgaagaaaat aaattcggca ggtccttcag gtggtccgat gtttttaaat
    13681 aaatgcaaaa aaccttcttc ttcacctatc agcatatcct gatcgccgtc gccatccaga
    13741 tcgccaaaag ttggggctat tccatgaaat ccgaaatcgg aaatatttcc gaggttgcgc
    13801 gtaatgagtg tatatgctgg cgcatcggca gtgccggtat tttgtaacaa tgctattttc
    13861 ccaatttgat cgccatcgtc gaaatattca taattgccta ctatgaggtc taatagtcca
    13921 tcataattat aatcgaaaaa cacaggaaaa gcgccatcac caaaatcaag catagtgctc
    13981 accatcaacg tatcttgtct ataatcgaaa acataagtat catcggtaga aatattttga
    14041 taataccata catttttaaa attctctgaa ctattgatat tatttggtgc tacaaccata
    14101 tctttcgctc catcattatt gatatcaatt agaaatggtg ccggaaatgc gggtaaatca
    14161 tatggcttgt cgtagcttgg aaaggtggta tcctgatcag taatatatgc caattcacta
    14221 tcaccgccgt ttgttaattt atttaataca tcaaatgcaa gatcgccaag tacaacatcc
    14281 atgtcgttat cagcatcttc atcgaatatc ataaatgtag atcctgcgtg cagaccatcg
    14341 gcagtgcgca caattccttt gcaatcgtaa ttaagatcaa caccgtaggt gattccgctt
    14401 tcataaaaat ttccccagca ggaattgata tgttccaatg cccaagtgcc gcatggtaaa
    14461 ccatcttcca cggcttgatt gtcataatga tcaacaatgc cgccgatcat attaaaagtg
    14521 atcacatcga tatcaccatc ctgatcgata tctgcaaaac cgggaatgtc tatataacca
    14581 acaggaagat caaagaaaaa tcctgcttcc gtgaattgca atttactcgc atcttccgta
    14641 aaatttatac tacctccatc tgatgcagca agatatgttt ttattccatt atcatagccg
    14701 catataatat cttctaatcc atcgcaatta taatccagca aaattgccca atctcgcata
    14761 gcaggaaatc gtgaagcgta ttcagcgcga tattcataat tgatttcgcc tgtgccacct
    14821 cggttgatga atatcatggg tatatttcca ctcctgtcga aaagaaacaa atcctttttt
    14881 ccatcctgat ttatgtctat ggatgaaaat tgcgggttat tcaatccacc tgcaaaggga
    14941 ttctgataat ttgttgtccc ttgcaatacc ggaacattgt agttatacat ataatgctgg
    15001 gctacagcac tgctataaac caaaatgctt actgctaaag ggaaaaggag cttttttaac
    15061 atgcgcacag aatgaatgat aaagatacaa caagcatgtt gttttgttgc tagcatcagc
    15121 gtttttgaca cagcggtgat aggggtttgt aaagtttggc acggaattct atttatggat
    15181 agtaacatgc tattacatca caataaaaaa gcgagctatg aaagcgaaaa atgtgcaact
    15241 tatcgcaggc gcagagggct
//
LOCUS       DAOQOU010000062        15252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_1971_length_15252_cov_6.144897, whole genome shotgun sequence.
ACCESSION   DAOQOU010000062 DAOQOU010000000
VERSION     DAOQOU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15252
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1971_length_15252_cov_6.144897"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLM90_07090"
     CDS             <1..1396
                     /locus_tag="PLM90_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HMZ89173.1"
                     /translation="IIQRKNVSINRLVHNVVLTQRNLTLYCDSAFLYKSENIARIYGH
                     VHLSQSDSIDAYGDSAIYDGNTRMAHLFGNVRLTDNSMTLTSKDLSYDMSSRVATYVN
                     GGKVTDGEAVLTSRTGYYYAFTNDAFFRNDVVLKHPKYQLEADTLQYNTGTETAFFHG
                     PTRITNENSTVLCRKGYYQTNTGIAVFHDQVTLNNPPQKLLADSIYYNRETGIGKAFR
                     HIVFTDTAQQVIQFSNVAEYDEKNNSIFSTDGSVAGYVMEDDTLFIGGDTIRTAQDSL
                     GRKTMYVFRNVKIYKSDLQGLCDSLSYSDADSTLRMYGLPIMWSDSTQFTADTMHLVM
                     LHQQLNQVQLFSNGFIINEDDSLIYNQIKGRNIYGYFSDNDLKKIKAIGNGESVYFGK
                     DDANRYIGVNTLYCSEINIYLSDKKFHRIAFKNAPDSEFQPMQNINLKDYQLKNFNWR
                     YEERPSSLDDLLKD"
     gene            complement(1404..2624)
                     /locus_tag="PLM90_07095"
     CDS             complement(1404..2624)
                     /locus_tag="PLM90_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMZ89174.1"
                     /translation="MKMKIKAPLQPIRSAITLEGSKSISNRLLLMQALCTSGFEIRNL
                     SPSDDTTTLQRLLKQQPSISDVGAAGTTMRFLTAYYSTLEGERMLTGSARMQQRPIGV
                     LVEALEKLGADITYIEKKGYPPVRIKGKELPGGTIRIRADISSQYISALMMIGPTLKK
                     GLTLILDGKIASAPYIQMTLSTMQELGIDCKWEGATITIKNGSYRSVAMQAESDWSAA
                     SYHYSICALSPGSHMRISGLFEKSLQGDSVLPDIYAKLGVTTFFEGKEMIISNTGNCT
                     SLLDQDYSDCPDIAQTVAVTCAALGIPARFTGVESLRIKETDRTAALQSELAKFNVTF
                     EEREPGEWILSGKINPVEATISTYEDHRMAMSFAPLALVAPSITIEEPEVVKKSYPSF
                     WYDMARLGFVLENI"
     gene            complement(2624..3685)
                     /gene="aroB"
                     /locus_tag="PLM90_07100"
     CDS             complement(2624..3685)
                     /gene="aroB"
                     /locus_tag="PLM90_07100"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HMZ89175.1"
                     /translation="MVLQTASYPIYIDSSPEHHGALSAWIIAQQYEQLFVLCDGETEK
                     HCYPLLKPLLPEHQIFTIPSGEAYKNINTCITIWQSLAKCIAGRKSLLINLGGGVIGD
                     MGGFAASTYKRGIDFIQIPTTLLSQVDASVGGKLGIDLDGVKNLVGVFRDPKAVFISP
                     AYLTTLSERQVKSGFAEMLKHGFIADADYLRTLHNADLHDVSLVQQLIERSVRIKKDV
                     VEKDPYEKGLRKILNFGHTIGHAVETWSLLNDEDPLLHGEAIAIGMICEAQLSTVICG
                     FSESELMEVTNMLMHRYGKYPLEKVSVNALMDIMQLDKKNSQQKIRFSLLQHIGLCQY
                     DLVATDDQIAAALQFYCGL"
     gene            complement(3685..3972)
                     /locus_tag="PLM90_07105"
     CDS             complement(3685..3972)
                     /locus_tag="PLM90_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HMZ89176.1"
                     /translation="MIIRIVKMTFDHRKVEDFLRIFSDAQHKIEAFPGCKGVDLTRDV
                     LQSNIFMTISIWENLASLDEYRKSELFKTTWSATKLLFSERPDAWSVEKIR"
     gene            complement(3969..4742)
                     /locus_tag="PLM90_07110"
     CDS             complement(3969..4742)
                     /locus_tag="PLM90_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HMZ89177.1"
                     /translation="MPIVTLTSDLGVKDYYVAVLKGKLLSGAPQALIVDISHEIAPFN
                     IQEAAFVMRNACFHFPPSTIHLVSVHAESRSPVKAVAIRMRDQYFIGMDNGLFSLMVE
                     DAPDQIVEIPISHGQQSSFAAKDILVHAAISLLQGKTLQQIGTPLGSLETKTYLRPPD
                     NAYLIRANIAYIDRFGNLIINITKERFEKLRGGREFVINYKRTEEIREISSTYTDVPE
                     GEKLALFNSSGYLEIAIHKGNASQLLGLHLDNTIQIEFE"
     gene            4786..5787
                     /locus_tag="PLM90_07115"
     CDS             4786..5787
                     /locus_tag="PLM90_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMZ89178.1"
                     /translation="MHERSKLPIKLIEVTIPLDGVRPIDFYGIHNARLGIIKKTFPFL
                     NISARGSELKVRGNEKDIEDFKHRIDLVINHLEKFNTISDSNLQSILFGVPETELVDL
                     PELPDDVIVHGPSGYLVRARTPNQKKMVSLATANDIVFAIGPAGTGKTYTAVALAVKA
                     LKEKVVKRIILTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALDDMLPIEKLNLYMQ
                     NRVIEIAPLAFMRGRTLDHSFIILDEAQNATDAQLKMFLTRIGPSAKCIITGDLTQID
                     LPRNQQSGLFTSLRILKDIDGIGTIFLNENDVVRHRLVKQIIKAYDVEKSRQEHHGE"
     gene            complement(5802..6446)
                     /locus_tag="PLM90_07120"
     CDS             complement(5802..6446)
                     /locus_tag="PLM90_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMZ89179.1"
                     /translation="MENKTTYEQLLINNKDWVTEKLAEDPSYFGRLSVGQNPPFLYIG
                     CSDSRLPIDLFTGADPGKIFIHRNVANQVFLNDMNLLSVLEYAVKVLKVEHVIICGHY
                     KCGGVEAAYSGTTMNIVENWTMNIRDIAMEYKKELDAIEDYQERLNRLSELNVIRQLR
                     QLCKTSVMHAAFNEGAYPKLHGWVLDIAHGLINELPLPIDEWKEYGLLPAGYQS"
     gene            6609..7565
                     /locus_tag="PLM90_07125"
     CDS             6609..7565
                     /locus_tag="PLM90_07125"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HMZ89180.1"
                     /translation="MSGFLDPVVLFFILGALAGVLKSDLKVPEAFYHTLSIYLLLAIG
                     IKGGIELYHANPSGILLPAIATITAGITVTLIAALLLKRIAKFNNPDVWSIAGHYGSV
                     SAVTFAVILTFLNDKQVPYEEFMTVLLVTLEIPAIAFAIFAYKSSVSSGKIEIRKLAH
                     EVFFGKSILLLLGGLGIGYYTAAANNQQLNFFFFDLFKGFLAIFMLEMGIVASARFAD
                     LKKVGISLILFGVMMPLIGASIGILAGNIVGLSVGGTAVLATMCASASYIAAPAAMRI
                     AIPDANPTYSLTAALGITFPFNIIIGVNLYYRMSVIVDGILN"
     gene            complement(7582..8103)
                     /locus_tag="PLM90_07130"
     CDS             complement(7582..8103)
                     /locus_tag="PLM90_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ89181.1"
                     /translation="MANWKKPQPGEVFPYQEKYIALVEGDDVLEMLSQHVQQTIDMLA
                     GIDEQKALFRYAPGKWSVKEVLQHLIDTERIMCYRALRFARKDKTPLPGFEENDYAPA
                     SMADNRSMVSLLQEFAAVRHATIMLFESFTDDMIARTGTGNGKEISVNALGFIIVGHE
                     LHHMKLFKEKYLI"
     gene            8237..8722
                     /locus_tag="PLM90_07135"
     CDS             8237..8722
                     /locus_tag="PLM90_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89182.1"
                     /translation="MKKVFDSVKTALGETTGVLGEGYLAATDLLKETLSNFYDVKDLT
                     KEKMASITNDVIALAPIIEQTGYRAKEINIGVGIPPRMVFHFEKFADIKDEEIKRILA
                     ENKDKKLLSVLVNTLVSADAFQQKITLGSFSFNEISIELSVPPEVNVKFVNAATQQTV
                     I"
     gene            8725..9990
                     /locus_tag="PLM90_07140"
     CDS             8725..9990
                     /locus_tag="PLM90_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ89183.1"
                     /translation="MDIISLLTRLAFTAIFYFIPDIFIHGAVKASFTKNPKRALKRYW
                     SLNIALFFITSSGFLLMQFMSPDFARINLYFFGIFVSLLFAKLIVAIILLIAEDIWRI
                     PYSLFTRKKRNVESTPEKAPLLISRRKFIAKSALIAGALPFTGFMYGIFKGRYRFTIH
                     RETLYYSNLPDAFDGFTITQLSDLHMGSWDTNAKYHIQTLVDEANALKSDLILFTGDI
                     VNSRADEMSGWYEIVKQLQAPFGKISILGNHDYGDYVNWKTPEAKAANLALVKNIHPA
                     IGFNLLLNENTSIEKEGAKIYIAGVENWGKGDFPKFGDLDLAYKGIPNDAFMLLMSHD
                     PSHWDAKILEHPRLPNLTLSGHTHGFQMGIEVPAIRFSPSQFIYPQWAGLYTKKDTHI
                     YVNRGLGTVGYPGRLGIWPEITQITLRKK"
     gene            10007..10639
                     /locus_tag="PLM90_07145"
     CDS             10007..10639
                     /locus_tag="PLM90_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89184.1"
                     /translation="MKYIIPVILLAVTLSCEKKEKQFDADGVLLAQFLESDSGSGLDF
                     SPMQNDDSSRVINFTPTIYNFKKKPYTGKVTAYDDKQRKIFDGNLNDGLPEGNWIFYY
                     ASGVVQIEGTYTKGLETGIWKNYYTVDKPKVIKQYDQSGYLLMRKEFYDTGKLKNYQN
                     VKCPQYGDIARRVQFKYNGAIEYLDAERELGKLDPAELQTVLLNDKLMVK"
     gene            10702..11355
                     /locus_tag="PLM90_07150"
     CDS             10702..11355
                     /locus_tag="PLM90_07150"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HMZ89185.1"
                     /translation="MRTLLLFVIACSVAAACESSDGMTTDETDEIVVKEVNIDSIDHC
                     YLVGKQAEAKAFCKSEGYNKHYCFLLDMSRHSGRYRFFIWDFDSNKVVDSALVSHGCG
                     ISNWGDDGTADAPVFSNEYESHCSSLGKYRVGERAWSQWGINIKYQLYGLDSTNSNAN
                     GRRIVLHSWEFVNDIEVYPDGSPEGWGCPAVSNAFMARLDTRLKKSTEPVLLWMYTH"
     gene            complement(11356..13398)
                     /locus_tag="PLM90_07155"
     CDS             complement(11356..13398)
                     /locus_tag="PLM90_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ89186.1"
                     /translation="MYGQTPISGVINTYYEVTGIDKCNNSITLTATPIGLAVGDNVAM
                     IQMRGVDADADNDNTYGSILNYAKSGVYELFTVQAISFNVVTLNEVIEKLYSIPGRVQ
                     LVRIPEYTDVEVTGTVTGLPWSGTVGGVVALIANGTLTLSADIDASGIGFRGAGVVLN
                     TPCLTGSPVGFDGYVTTAGEDKAGKKGEGISENGDDRYARGAPANGGGGGNDRQTGGG
                     GGSSYAPGGGGGQLLFPPAGSCGGSYPGIGGNPLIYDNAENRIFMGGGGGQGSSNLGT
                     GTSGGNGGGIIIIIANNIEGNNFFIRSNGMSVTTTAFDDGAGGGGGAGAVLIDAASIS
                     SVVNVQVHGGDGGDVDNTFDGVNCVGPGGGGSGGVLWMPSGAVPPNVNLDAGGGSSGI
                     TVGEGAASPCFNSVNGATAGSNGNYVGGLALPIADQLYVELTLDMIPDDAVVCQGNEL
                     FMSAVATGTGSVSYHWNDEDASTTPDVTVIPETDMTYTVIIEDDLGCQLIGEVVVDVI
                     DTVEITAFPDTSLEMGNYMTLYSNLDDTYTYLWSPDYNIDDVTAANPVINPYETTTYC
                     VTATHPTGCSSTDCITILVAAGVAFPNAFTPNDDGINDVFRIPPVANLCEDIQYFKVF
                     DRWGQVVYDYYATTDSEGWDGTSGGIPQEIGTYVYLIKMLCDGENRTYSGALHLMR"
     gene            13552..14082
                     /locus_tag="PLM90_07160"
     CDS             13552..14082
                     /locus_tag="PLM90_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89187.1"
                     /translation="MTPISVTKQVENTINLIPSELSHFDEQAFFNRNGDTWSPAEQVK
                     HLELAVKPLILAFRLPSFVLRLLFGKPNRPGRSYDELLTKYHLKLSEGGKASAPYVPK
                     ALDTAAEKSTVINDCLTRHHKMLKAANSYNDTNLDKYLLPHPLLGKITLREMLFFTDF
                     HIRHHLQSIQQLYGNK"
     gene            14069..14551
                     /locus_tag="PLM90_07165"
     CDS             14069..14551
                     /locus_tag="PLM90_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ89188.1"
                     /translation="MATNEAARIAKLFSDWYDGTPWTDIKMTDILMDITPEIAAAHPI
                     PGANSIWQLVYHCLGWRINVLQKIQGAEFKSPEDNYLSEPQDFSASAWQQLLKDYAQN
                     EKDWQLYLSNINDDILDSGYLPSKTKFTCYEVIHGLLHHDNYHFGQILMLKKILISKQ
                     "
     gene            complement(14555..>15252)
                     /locus_tag="PLM90_07170"
     CDS             complement(14555..>15252)
                     /locus_tag="PLM90_07170"
                     /inference="COORDINATES: protein motif:HMM:NF027968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ89189.1"
                     /translation="DSHDTQRFTSFLHNPDIARFHTWGEYFNATKSTNLAYNVGKPTE
                     EEYKRQMLMALFQYAYVGAPMLYYGDEVGMWGANDPDCRKPMVWQDMNYQPEQKLSSQ
                     EVRTPVDTVEVNTELLDYYRKLGKLRNMFDVLQTGDYQTIIADNKSGVYVFARSNNNL
                     TVYVVLNNSAKEQKVKLNMPGMRYYYQPLESEITKIKKANVALTIPAVSGTVVFPFTL
                     DECVNVTPEMMGK"
BASE COUNT         4579 a         3342 c         2979 g         4352 t
ORIGIN      
        1 aattattcaa cgaaaaaatg taagcatcaa tcggctggta cataatgtag tgttaacaca
       61 gcgaaatctg acattatatt gcgatagtgc ctttctttat aagtctgaaa atatcgccag
      121 gatttatggc catgtgcatc tcagtcagag cgatagtata gatgcatacg gagattcagc
      181 aatatatgat ggtaatacac gcatggcaca cctatttggc aatgtgcgcc ttaccgataa
      241 ttcgatgacg cttacttcca aggatttgag ttacgatatg tcatcgcgtg ttgccactta
      301 cgtaaacgga ggaaaagtta ccgatggcga agccgtgctg actagcagaa caggatatta
      361 ttatgcattc accaatgatg cattttttag aaatgatgtt gtgctcaagc acccgaaata
      421 tcaactcgaa gcagatacac tccaatacaa taccggaacc gaaacggcat tcttccatgg
      481 cccaacacgt attaccaatg aaaacagtac cgtattgtgc cgaaaaggat attaccaaac
      541 aaataccgga atcgcagttt ttcatgatca ggtaaccctc aataatccac ctcagaaatt
      601 actcgccgac agcatttact acaaccgcga aacaggtatc gggaaggcat tcaggcatat
      661 cgtgtttacg gataccgctc aacaggtaat tcaattcagt aacgttgccg agtacgacga
      721 aaaaaataat tcaatctttt ctaccgacgg atctgttgca ggctatgtga tggaagatga
      781 tacgctattc attggcggcg ataccatcag aactgcgcag gatagtttag gtcgcaaaac
      841 catgtatgtt tttcgcaatg tgaaaatcta caaaagcgat ctgcaagggc tgtgcgattc
      901 attgagttat tctgatgcgg actctacctt acgtatgtac ggactgccta ttatgtggag
      961 cgatagcaca caatttactg ccgacaccat gcacctggtg atgctgcatc aacaattgaa
     1021 tcaagtgcag ttattttcga atggatttat tattaatgaa gatgacagcc taatctataa
     1081 ccaaattaaa ggcagaaata tatacggata tttttcagac aatgacctca aaaaaatcaa
     1141 agcgataggg aatggcgaaa gcgtgtattt cggcaaagat gatgctaaca gatatattgg
     1201 agtaaatacg ttgtattgca gtgaaattaa tatttatcta agcgacaaaa aattccatcg
     1261 catcgcattt aaaaatgcac cggattcaga attccagccg atgcaaaata tcaatttaaa
     1321 ggattaccag ctcaaaaatt tcaactggcg ctacgaggaa cggccttcgt cgcttgacga
     1381 tctgcttaag gattaatttg gaatcagata ttctccaaca caaatcccaa tcttgccata
     1441 tcataccaga atgaagggta tgatttcttt acaacttccg gttcttcaat ggtgatagat
     1501 ggcgcaacca aagcaagcgg cgcaaaactc attgccatgc gatgatcttc atacgtactg
     1561 atggttgctt caacaggatt aattttccct gataatatcc attcgccagg ctcacgttct
     1621 tcaaatgtta cattgaattt tgccaattca gattgcaagg ctgctgttct atccgtttct
     1681 tttatacgca aactttccac gccggtaaat cttgcaggta tacccaaagc agcacaagta
     1741 acagcaactg tttgtgctat atcgggacaa tcactataat cctgatcaag caaggaggtg
     1801 caatttccgg tatttgaaat tatcatttcc tttccttcga aaaatgttgt cacgccaagc
     1861 ttagcataga tatcaggaag cacgctatcg ccttgcaaac tcttttcaaa aagaccagat
     1921 atgcgcatgt gtgatcccgg cgataatgcg caaatactgt agtgatatga tgcagcactc
     1981 caatcgcttt ctgcctgcat agcaacagac cgatagctgc cattttttat cgtaatagtt
     2041 gctccttccc atttgcaatc aataccaagc tcctgcatgg tgctcaaggt catttgaata
     2101 tacggcgcgg atgcaatttt cccatcaaga atcagtgtga gacctttttt caatgtaggt
     2161 ccaatcatca tcaatgccga aatatactgc gaactgatat ctgcccgaat gcgaatggtt
     2221 ccacccggta attccttgcc ttttattctc acaggcggat accctttttt ctcaatgtag
     2281 gtaatatcgg ctcccaattt ttctaaagcc tccaccaata caccgattgg tctttgctgc
     2341 atgcgtgcac tgcctgtaag catgcgttca ccttcaagtg tgctgtaata tgctgtcaga
     2401 aaacgcatgg tggttcctgc tgcaccaaca tcactaatgg aaggttgttg cttcaataaa
     2461 cgctgcaatg tcgttgtatc gtcagaaggg gataaattgc gtatttcaaa tcccgaagtg
     2521 cataatgcct gcatcaacaa caaacgattg ctgatgcttt tacttccttc aagtgttatt
     2581 gcactgcgaa taggctgcag tggcgccttt attttcatct tcatcacaaa ccacaataaa
     2641 attgtaaggc tgcagcaatc tgatcatcgg tagcaacgag atcgtactga cacaaaccaa
     2701 tatgctgcaa taaactaaat cgtatttttt gttgcgaatt ctttttgtcg agttgcatga
     2761 tatccatcaa tgcatttaca gacacttttt caagcggata ttttccatac cgatgcatca
     2821 acatgttcgt cacctccatt aattcgcttt cagagaaacc acaaatcact gtcgagagct
     2881 gtgcctcgca tatcatgcca atagctatcg cttcgccatg caacagcgga tcttcatcgt
     2941 ttaataaact ccaggtttca acggcatggc caatggtatg tccgaagttt aatattttgc
     3001 gcaacccctt ttcataggga tctttttcta caacatcttt ttttattcga acagaccgtt
     3061 caatcaattg ctgcaccagc gatacatcat gcaaatctgc attatgcaat gtccggagat
     3121 aatctgcatc cgcaataaac ccatgcttca acatttccgc gaatccactt tttacttgcc
     3181 tttctgacag agtggttaaa tatgcaggcg aaataaaaac agctttagga tcgcgaaaca
     3241 cgcctacaag gttttttacg ccatcgaggt cgattccaag tttaccacca acagaggcat
     3301 ctacttgcga taataaggtt gttggtattt gaataaaatc aattccacgt ttgtatgttg
     3361 atgcagcaaa tcctcccata tcgccaatca ctccaccacc taaatttatc aataagcttt
     3421 ttcttcctgc tatacatttt gcgagcgact gccatatggt aatacaggta ttaatatttt
     3481 tatatgcctc accggaaggt atggtgaaaa tttgatgttc cggcaataaa ggtttcaata
     3541 aaggatagca atgcttctct gtttcaccat cgcaaagcac aaataattgc tcatattgtt
     3601 gcgcaattat ccaggctgaa agggcaccat gatgttcagg tgaactgtcg atataaattg
     3661 gataggaagc tgtttgcaat accattatct aattttttca actgaccagg catcggggcg
     3721 ttcagaaaac aacaattttg ttgccgacca ggttgtttta aataattctg atttgcgata
     3781 ttcatccaga gatgccagat tttcccaaat actaatggtc ataaaaatgt ttgattgtag
     3841 tacatctctg gtaaggtcaa cacctttgca gcccggaaat gcttcaatct tgtgctgtgc
     3901 gtccgaaaat attcggagaa aatcttcaac ctttcggtgg tcgaaggtca tttttacaat
     3961 gcggatgatc attcaaattc tatttgtatg gtgttatcta aatggaggcc taacaactga
     4021 cttgcatttc ctttgtggat ggcgatttcc agatagccgg aagaattaaa taaggccagt
     4081 ttctcccctt ccggcacatc tgtatatgtg gatgaaattt cgcgaatttc ttcggtacgt
     4141 ttataattga tcacaaattc tcttcctcca cgcaattttt caaaacgctc ctttgtgatg
     4201 ttgatgatca ggtttccaaa tctatcgata taagctatgt ttgccctaat gagatatgca
     4261 ttatccggcg ggcgcaaata agtctttgtt tccagcgatc ccaaaggagt gccgatttgt
     4321 tgtaaagttt taccttgcaa taaactgata gcagcatgca ctaatatatc tttcgcagca
     4381 aacgaagatt gttgtccatg tgaaataggt atttccacaa tctggtcggg agcatcttcc
     4441 accataagtg aaaacaatcc attatccata ccgataaagt actgatcacg catgcgaatg
     4501 gcaacagcct taacgggact gcgagattcg gcatgaacgc ttacaagatg aattgtagac
     4561 ggcgggaaat gaaaacatgc attgcgcatt acaaaagcag cctcctgaat attaaatggg
     4621 gcgatctcgt gggaaatgtc cacaatcaag gcttgtggtg cgcctgacag taatttcccc
     4681 tttaacactg ccacatagta atcctttaca ccgaggtctg aggtaagggt tacgataggc
     4741 ataaatggga actaagggct gctggttagt tcaaaaaagg taaatttgca tgaacgttca
     4801 aaattaccaa taaaattgat tgaagtaacc attcctttag acggtgtgcg gccgattgac
     4861 ttctatggta tacacaatgc gcgccttggc atcatcaaaa aaacattccc atttctcaat
     4921 atctccgccc gaggtagtga gctgaaagtg cgtgggaatg aaaaagatat cgaagatttt
     4981 aaacatcgca tcgacctggt catcaaccac ctcgaaaaat ttaatactat cagcgacagc
     5041 aatctgcaaa gcatcttatt cggcgtgccc gaaactgagc ttgtcgatct gcctgaatta
     5101 cctgatgatg tgattgttca cggtccatcc ggttatctgg ttagagcacg cacacctaac
     5161 cagaaaaaaa tggtaagcct ggcaactgcc aatgatattg ttttcgccat tggcccggcc
     5221 ggaacaggaa aaacgtatac cgccgttgca ctcgccgtga aggcattgaa ggagaaagtt
     5281 gtaaaacgca tcatcctcac acggcccgcc gttgaagccg gcgaaaattt aggatttctt
     5341 cccggtgatc tgaaagaaaa aatcgatcca tatctgcgac cattatatga cgccctggat
     5401 gatatgttgc cgatagaaaa actgaattta tacatgcaaa atcgcgtgat agaaattgca
     5461 cctttggcat tcatgcgcgg aagaacactc gaccattcat ttattattct tgatgaagct
     5521 cagaatgcta cagatgcaca gttgaaaatg tttcttacac gcatcggacc cagcgcaaaa
     5581 tgtattatta ccggcgatct tacccaaatc gacttgccga gaaatcagca atcgggttta
     5641 tttacatcgt tgagaattct gaaagatatc gatggcattg gcaccatttt cctcaatgaa
     5701 aatgatgttg ttcgtcaccg cctcgtgaag caaatcatca aagcatacga tgtagaaaaa
     5761 tcgcggcagg agcaccacgg cgagtgataa ttaaagcagc attaggattg ataaccggca
     5821 ggtaataatc catattcttt ccactcatca atcggaagcg gaagttcgtt tatcaagcca
     5881 tgtgcgatat ccaataccca tccatgcaat tttggatagg cgccttcatt aaatgctgcg
     5941 tgcatcacgc ttgttttgca taattggcgc aattgcctga ttacatttaa ttccgagagt
     6001 ctgtttaacc tttcctgata atcttcaatc gcgtcgagtt cctttttata ctccatggca
     6061 atatctctga tattcatggt ccagttctct acaatattca tggtagttcc tgaataggca
     6121 gcttccaccc caccacattt ataatgtcca cagatgatca catgttcaac cttcaacact
     6181 ttcactgcgt actccagcac cgacaagagg ttcatatcat ttaggaatac ctgatttgcc
     6241 acattcctgt ggatgaagat tttacccgga tcggcaccgg taaaaaggtc aattggaagg
     6301 cggctatcgc tgcaaccgat atacagaaat ggtggatttt gcccaactga caaccgacca
     6361 aaataagacg gatcttctgc caatttctcg gttacccaat ccttgttatt gatcaataac
     6421 tgctcgtagg tggtcttgtt ttccatatga taatggtcaa aagtagtata aacagactgg
     6481 tttttccagt gtcaggtgaa aatagcccct atcaagggct gttaacattc ataaaatctt
     6541 gaaaatgtta aaatttaacg ccgattgaac attttataag aggcactgtt tcggacaaaa
     6601 tcacacatat gagcggtttc cttgaccctg ttgtattgtt cttcattctc ggtgcactgg
     6661 caggcgtttt aaaaagcgat ttaaaagtcc cggaagcatt ttatcacact ttaagtattt
     6721 acttgttatt agccatcggc atcaagggtg gcatagaact ttatcatgca aacccttcgg
     6781 gaattttatt gccggcaatt gcaacgataa cggcaggaat aacagtaaca ctgatagcgg
     6841 ctttattgct aaaacgtatt gcaaaattca ataatccgga tgtatggtca attgcaggtc
     6901 attacggttc tgtgagtgcg gtaacatttg cagtaattct cacattcctt aatgataaac
     6961 aagttccata cgaggaattc atgacagtcc tgttggttac cctggaaata ccggcaattg
     7021 cgtttgcaat ttttgcatat aagtcatctg tcagttcagg caaaattgaa attcgaaagt
     7081 tagcacatga agtattcttt ggtaaaagta ttttattgct gcttggcgga ttaggcatag
     7141 gatattatac ggccgcagca aataatcaac aactcaactt ttttttcttt gacctattca
     7201 aagggttcct tgcaatattc atgcttgaaa tgggaattgt tgcgagtgca cgattcgctg
     7261 acttgaaaaa agttggcatt tcgctaatac tgtttggtgt aatgatgcca ttgattgggg
     7321 catcaatagg aattctcgca ggaaatattg taggcttgtc tgtcggagga actgcagtcc
     7381 ttgcaacgat gtgcgcaagt gcatcatata tagcagcccc tgcagcaatg cggattgcta
     7441 taccggatgc aaatcccact tacagcctta ctgccgcgct tggtattacc ttccctttta
     7501 acattattat aggtgtaaac ctatattatc gaatgagtgt tatcgttgat gggatattga
     7561 attaatacca atactttctt ttcaaattaa atacttttct ttaaacaact tcatgtgatg
     7621 cagctcatga cccacaatga taaagccaag tgcgttcacc gatatttcct tgccattgcc
     7681 tgttccggtg cgtgcaatca tatcgtctgt aaaagattca aataacatta tggtagcatg
     7741 acgcaccgct gcaaattcct gtaacaaaga aaccatgctc ctgttatcgg ccattgatgc
     7801 cggagcataa tcattttcct caaaaccggg taaaggagtt ttgtctttgc gggcaaatcg
     7861 caaagcacga tagcacataa tgcgctctgt gtctatcaga tgttgcaaca cctctttcac
     7921 gctccacttt ccgggggcat aacgaaacaa ggccttctgc tcatctatcc ctgcaagcat
     7981 gtcgatggtc tgttgaacat gttgcgacaa catctcaagc acatcatctc cttctaccaa
     8041 ggctatatat ttttcctgat atggaaatac ttctccgggt tgtggttttt tccaattcgc
     8101 cattttttca acttttatca atgatcacaa aaatgaattt ctttcggcaa agtaacaagc
     8161 atgatctaaa ctattattgt taaaatcctt ccatctctat aggacaccaa tctcccgcaa
     8221 cgcttatctt tgcggcatga aaaaggtttt cgactctgtg aaaacagcac ttggtgaaac
     8281 aacaggtgta ctgggcgaag ggtatctggc agcaacagac ttactaaagg aaacattatc
     8341 caacttctac gatgtgaaag acctcaccaa agagaagatg gcttccatca ccaacgatgt
     8401 tatagcactg gctccaataa tcgaacaaac cggttacaga gcaaaagaaa tcaatatcgg
     8461 cgttggcata ccacctcgaa tggtatttca cttcgaaaaa tttgccgaca tcaaagatga
     8521 agaaatcaag cgtatactcg ccgaaaataa agacaaaaaa ttgcttagcg tgctggtaaa
     8581 taccttagta tcagcagatg catttcagca aaagataacc ttgggcagtt tttcatttaa
     8641 tgagatcagc atagagttga gtgtgccacc tgaagtaaat gttaaatttg tgaacgcagc
     8701 cacccaacaa actgttatct gatcatggac attatttcgc tgcttactcg actggcattt
     8761 acagccatat tttatttcat cccggatatt ttcatccatg gcgccgtaaa ggcctcgttt
     8821 acaaaaaatc cgaaacgggc attaaaaaga tattggtcac taaatattgc gcttttcttt
     8881 attacgagca gcggatttct gctcatgcaa tttatgagtc ccgactttgc caggatcaat
     8941 ctgtactttt tcggtatatt cgtaagcctc ttatttgcca aactcatagt agcgataatt
     9001 ttattaatcg ctgaggatat ctggcgcata ccttacagtc ttttcacacg aaaaaaacga
     9061 aatgttgaat ctacgcctga aaaagcacca ttgctgatta gtcgcaggaa attcattgca
     9121 aaatccgcac tcattgccgg agccttaccg tttaccggat ttatgtatgg catttttaaa
     9181 ggcagatatc gatttaccat tcatcgcgaa acattgtatt attctaatct ccctgatgca
     9241 ttcgatggat ttaccatcac acaactcagc gatctgcata tgggttcatg ggataccaac
     9301 gcgaaatacc atattcagac tttggttgat gaggcaaatg cactaaaatc tgacttaata
     9361 ttatttaccg gcgatatcgt gaacagcaga gctgatgaaa tgtcgggatg gtatgaaata
     9421 gtaaaacaat tacaggcacc tttcggtaaa atttctatcc ttggcaatca cgattatggc
     9481 gactatgtga attggaaaac gcctgaggca aaagcggcaa atctggcatt ggtgaaaaat
     9541 attcatcctg ccataggttt caatttattg ttaaatgaaa atacatcaat agaaaaagag
     9601 ggagccaaaa tctatattgc aggtgtagaa aactggggga aaggtgattt tccgaagttc
     9661 ggcgatctcg acctcgcata taaaggaatt cctaatgatg cattcatgct tctcatgtcg
     9721 cacgacccat cgcattggga tgcaaaaata ttggagcatc cgcgtttacc aaatctcaca
     9781 ttgagcgggc atacacacgg atttcaaatg ggtatagaag tgcctgcaat tcgattctct
     9841 ccctctcaat ttatttatcc gcaatgggca ggactctata ccaaaaaaga tacgcatatc
     9901 tacgttaatc gcggactggg aacagtgggt tatccgggac gtttgggcat atggcctgaa
     9961 ataacacaaa ttacactccg taaaaaataa attgtaattt taaaatatga aatatattat
    10021 tcctgttatt ctgctcgcag ttaccttgtc ttgcgaaaaa aaagaaaaac aatttgatgc
    10081 agatggcgtc ttgttggcac agtttcttga atcagattcc ggaagcggac ttgacttcag
    10141 tccgatgcaa aatgacgata gttcaagggt gatcaatttt acgcccacca tttacaactt
    10201 taagaaaaaa ccatatacgg gaaaagttac tgcgtatgac gacaagcaac gaaaaatatt
    10261 cgacgggaat ttaaatgatg gtttacccga aggcaactgg atattttatt acgcttcagg
    10321 tgttgttcag attgaaggca cttatacaaa ggggttggaa acaggtatat ggaaaaatta
    10381 ttataccgta gacaaaccaa aagtgatcaa acaatatgat caatccggat atttattgat
    10441 gcgcaaagaa ttttacgata caggcaaatt gaagaactac caaaatgtaa aatgtccgca
    10501 atatggcgat atcgctcgtc gtgtgcagtt taaatacaat ggtgccatag agtatctgga
    10561 cgctgagaga gaacttggca aattagaccc ggctgaattg caaactgtat tgttgaacga
    10621 caaattgatg gtcaaataag ccgaccttac ttcaacataa tttaattacc atttctgcgt
    10681 tatgggtgaa aacccaaatt aatgcgcacg ctccttctct ttgtaatagc ttgtagtgtt
    10741 gctgctgcat gtgaatcatc tgacgggatg acaaccgacg aaacagatga aatagtcgtt
    10801 aaggaggtca atatcgattc tatagaccac tgctatttag tgggtaagca agccgaagcc
    10861 aaagcattct gtaaatcaga aggatataat aagcattatt gttttctact tgacatgagc
    10921 cgccattcgg gacgctaccg attttttata tgggattttg atagcaacaa agtagtggac
    10981 agtgcactgg taagtcatgg ttgcggtata agcaattggg gtgatgatgg cactgctgat
    11041 gcaccggttt tcagtaatga atatgagagc cactgttcat cattaggaaa atatcgcgtt
    11101 ggcgaaagag cgtggagtca atggggaatt aacataaaat atcaattata cggtctcgat
    11161 agcaccaaca gcaatgcaaa cggacgcagg atagtattgc atagttggga atttgttaac
    11221 gacattgaag tatatccgga tggctcaccg gaaggatggg gttgccctgc agttagtaat
    11281 gcatttatgg cacgactgga tacccggtta aaaaaatcaa ccgaacctgt tttactctgg
    11341 atgtatacgc attaattaac gcatcaaatg caatgctcct gaataggtgc gattttctcc
    11401 atcgcaaagc attttgatca gatacacata tgtaccgatc tcttgaggaa ttccgccgga
    11461 ggttccatcc catccttcag aatcagttgt agcataataa tcatatacta cctgtcccca
    11521 tctgtcgaat actttaaagt attggatatc ctcacacaaa ttcgctacag gaggtattct
    11581 aaagacatca ttgataccat catcatttgg cgtaaatgca tttggaaaag ctactcctgc
    11641 ggctactaaa atggtaatgc aatctgttga agaacaacct gtaggatgcg tggccgttac
    11701 acagtaagta gtagtttcat acggattaat aacaggattt gcagcagtta catcgtcgat
    11761 attataatcg ggtgaccata ggtaagtata ggtatcatca agattagaat acaaggtcat
    11821 atagttgccc atttcgagcg atgtatcggg gaaagcggta atttctactg tatcaataac
    11881 atctaccacc acttcaccga ttagctgaca tccaagatca tcttcaatga taacagtata
    11941 ggtcatatct gtttcaggaa tgacagtaac atcaggagta gttgaagcat cttcatcatt
    12001 ccagtgataa ctaacagatc cggttcctgt tgcaacagca ctcataaata attcatttcc
    12061 ctgacaaaca actgcatcat caggaatcat atcgagtgta agttcaacat ataattgatc
    12121 tgcaattgga agtgcaaggc caccgacata gtttccgttt gaaccggcag ttgcgccatt
    12181 aactgagtta aaacatggtg aagctgcgcc ttctccaaca gtaattcctg aagaaccacc
    12241 gccggcatca aggtttacgt ttggaggtac tgcaccggaa ggcatccaaa gaacaccacc
    12301 agagcccccg cctccgggac ccacgcagtt aacgccatca aaggtattat ctacatcacc
    12361 accatcgccg ccgtgaactt gaacatttac tactgaagag attgaggctg catcaataag
    12421 cacagcacct gctccgcctc ctccacctgc tccgtcatca aaagcagttg tggttacgga
    12481 cataccattt gatcgtataa agaaattatt tccctcaata ttattagcta taattataat
    12541 gataccacca ccattaccac ccgaagtacc tgtgccaagg tttgaactac cctgacctcc
    12601 gccgccaccc ataaagatgc ggttttcagc attgtcataa atgagcggat taccccctat
    12661 tcccgggtat gaaccaccac atgaacctgc aggagggaac aacaattgtc caccacctcc
    12721 accgggagca tagcttgatc cgccaccacc gccggtctgg cgatcgtttc ctccaccacc
    12781 accgttggcc ggcgcaccac gagcatacct atcgtcgccg ttttcgctga tgccttctcc
    12841 ttttttaccg gctttgtctt caccggctgt tgtcacatat ccatcaaatc cgactggcga
    12901 ccctgttaaa cacggagtat taagcaccac acccgcgcca cggaatccaa tgcctgaagc
    12961 atctatatct gcacttaaag taagtgtccc atttgcgata agcgctacaa ctccccctac
    13021 tgtaccactc cagggaagtc ctgtcacagt tcctgtaact tcgacatccg tatattccgg
    13081 tatgcgcacc aactgcactc taccgggaat gctgtataac ttttcaataa cctcattgag
    13141 tgtaaccacg ttgaaggaaa tagcttgaac cgtgaaaagc tcatacacac cgcttttcgc
    13201 ataattcaat atcgagccgt atgtattgtc attgtcggca tcagcgtcta caccacgcat
    13261 ctgaatcatg gccacgttat cacctaccgc taacccaata ggggttgcag tcaatgtaat
    13321 ggaattatta catttgtcga tacctgtgac ctcataatag gtgtttataa cccctgaaat
    13381 aggagtttgt ccgtacacgg acagcccaat tgtgaggagc acactacagg ttaaaatcct
    13441 aaacttcggc atattgcagt gtgtgtggtt ttttacgaag ataagtaatc gccacaaaat
    13501 tgcagatatt caacggattt tgggacttaa ttattcacat ttaccgggaa tatgacacca
    13561 atttcagtca caaaacaagt cgaaaacacc ataaatctga taccatcaga attatcgcac
    13621 tttgatgaac aggcattttt taatcgcaac ggcgatacct ggagcccggc agaacaagta
    13681 aaacaccttg aacttgccgt aaaacccctg attttggcct ttcgacttcc gagttttgtt
    13741 ttgcgtctcc tcttcggtaa gcccaatcgc cccggaagga gttatgacga gcttttgacg
    13801 aaatatcacc ttaagttgag cgaaggcgga aaagcatctg ccccatatgt gccgaaagca
    13861 ctcgataccg ctgctgaaaa atcgaccgta attaacgatt gtcttaccag gcatcataaa
    13921 atgttgaaag cagctaacag ctataatgat actaacctgg ataaatacct cctccctcat
    13981 cctcttttag gaaagatcac actcagagaa atgctgttct ttaccgactt tcatattcgt
    14041 caccacctgc aatcaataca acaactatat ggcaacaaat gaagctgctc gcatcgcgaa
    14101 attattttca gattggtatg atggcacacc gtggacagat atcaaaatga cagatatcct
    14161 catggacatt acacccgaaa tagctgccgc tcatccaata ccgggtgcaa acagtatctg
    14221 gcaattagta tatcattgtt tggggtggcg catcaatgta ttgcagaaaa ttcagggtgc
    14281 cgaattcaaa tcgccggagg ataattactt atccgaaccg caggattttt ctgcttcggc
    14341 atggcaacaa ttactgaagg attatgcgca aaatgaaaag gactggcagc tctatttatc
    14401 aaacatcaat gatgacattt tagacagcgg atatcttccg agcaaaacta aatttacttg
    14461 ttatgaagta attcacgggc tcttgcatca cgataattat catttcggac aaatacttat
    14521 gttgaaaaaa atattaattt cgaaacagta ataattattt tcccatcatt tcaggtgtaa
    14581 catttacaca ttcatctagt gtaaaaggaa atactacagt ccctgaaact gccgggattg
    14641 ttagtgcaac attagctttc tttatttttg taatttcaga ttccaatggt tgataataat
    14701 atctcatccc cggcatattc aactttacct tttgctcctt tgcactatta ttcaatacaa
    14761 cataaacggt tagattatta ttacttctcg caaaaacata aacgcccgat ttattatctg
    14821 caataatagt ttgataatcg cctgtctgta gcacatcaaa catgtttctc aacttgccaa
    14881 gttttctgta atagtctaac aattctgtat ttacctcaac cgtatcaacc ggtgttctta
    14941 cttcttgcga tgacaatttt tgctcgggct gatagttcat atcctgccat accattggct
    15001 tccggcaatc tggatcattt gctccccaca tgcctacctc atcgccgtaa tacaacatcg
    15061 gagcgcctac ataagcatat tgaaaaagcg ccatcaacat ttgccttttg tattcctctt
    15121 cagtgggctt gcctacatta tatgcgagat ttgttgattt agttgcatta aaatattcac
    15181 cccaagtatg aaaacgcgca atatccggat tatgcaaaaa agatgtgaag cgctgcgtat
    15241 cgtgactatc at
//
LOCUS       DAOQOU010000063        15088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2000_length_15088_cov_6.167033, whole genome shotgun sequence.
ACCESSION   DAOQOU010000063 DAOQOU010000000
VERSION     DAOQOU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15088
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2000_length_15088_cov_6.167033"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /locus_tag="PLM90_07175"
     CDS             complement(<1..2183)
                     /locus_tag="PLM90_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HMZ89190.1"
                     /translation="MRKLLSTLTLIALTISVSLAQMTIADARLQPVGTSVTVKGIALN
                     GPELGSIRYIQDATGNIAVYSATLVTGVQRGDSITATGPLFNYNSLLEISPASSVTIN
                     STGHPLPAPIDVTSLSGWVEGNEGKLLRISNATFVDAGTFSTAGSGTNYDVTDAAGTS
                     QIRVVTTTNIDGTPIPAEPVFITGIMSQYAPGGTGGYQLLPRDLADISTGGNPPVISS
                     VLTQTDITTSSFTVNFNTLYEGNTIIYYGTTMSLGSTASSAAMVTTHALNLTGLTAGT
                     VYYVKAASVSASNDTSYSAITAMATASNSSGQIKVWFNNPVDNSVSTGTNATFVNSAM
                     DDTVIWYLDHAKYSLDIAIYNIDNVNYIINSINDAYARGVDVRVICDNGVDDFNYGLI
                     NVGVGKKKKSPPDGSTNADGALYGIMHNKFIVVDGISVDPDDAWVLTGSMNFTDQQIK
                     MDKQNYLAIQDMSLAKAYRLEFDEMFGGKFGPDKSNNTPHEFMIGGKRVEVHFSPSDE
                     VETRMIERIGTADYDMHFAIYSFTRFGISYAIEDAVLDRGVFASGIWDETNADDSTAI
                     NVCEDAMGDRFEKYSGTNLLHHKYLIIDPNCPQSDPMVWTGSHNWSTSANSRNDENTI
                     VIHDATVANLFYQEYMQRYKDEGATDMVNAACEVSINNATQDEGEIRLYPNPATNQVT
                     IALGEVTNGNIKIFDISGKVMLSSEVHGLQYFTININELPAGMYFV"
     gene            complement(2265..2999)
                     /locus_tag="PLM90_07180"
     CDS             complement(2265..2999)
                     /locus_tag="PLM90_07180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMZ89191.1"
                     /translation="MILQHGARQFDLSNSRSIAIPYSTGEQVNCYYAPPFLVTPVVMG
                     TFIGDINQGSPVNYMNVQVNPHGNGTHTECIAHISNLPVNIQGVLKNHHFIAQIISVT
                     PDIIENGDTVLLSKNIIPILEKDIEAVIIRTLPNVSAKTTKAYSGTNPPYLEATAALS
                     FRESGIKHILIDLPSLDREEDGGALAAHKAFWHYPEAPRLDCTITELIFVPDAIADGI
                     YLLNLQIAPFCMDATPSNPVVYPMLK"
     gene            complement(3002..4660)
                     /locus_tag="PLM90_07185"
     CDS             complement(3002..4660)
                     /locus_tag="PLM90_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89192.1"
                     /translation="MKRIIPLIAFLGILMDSHAQLPFTTSSLYALGAAYLDRPYIRDM
                     ADIDSDGDMDLVGYIARDGNPTVLAWLENEGTPPFVQHDIAATEYGYYIHTTDFDMDG
                     DMDILAPIQLDGTVLYFYENHGDGDFVPHELTYLSGYYRNFAVSDLDGDLDPDILRIA
                     PSIGGVMFDVTWLENDGDGNVTSHIIDNINTPESFGAGDLDDDGDMDFVLGTANRVYW
                     YEKIVAGYTRHLVINSGDIFFNKIFIEDMDLDGDKDIISVSDYIIKQYENDGSENFTT
                     SVAYDPPGGAILIGLGDINGDSFPDMFGKWVITGNYHVINLLGNADFTFTLMDSIVPI
                     CGNEALTYGDLDRDGDTDYILSENCEVGPIKDVMFWELNGSTPLPCAPVEVLNVYTDS
                     LGLQATWSDTYADYYKLFAKCIDCPAPSPTISKTVYAPIGYLDEMLPCHTYKVKVRAF
                     CGGSVYGSPVLTVTTDGAACRQSEVDIESVLSVYPCPASHTLMISGIQIDVRAEIYAM
                     DLSGKTLRLENSANEIDIRMLPSGFYILTIELNGDLYRTQFIKQ"
     gene            4743..6101
                     /gene="mtaB"
                     /locus_tag="PLM90_07190"
     CDS             4743..6101
                     /gene="mtaB"
                     /locus_tag="PLM90_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HMZ89193.1"
                     /translation="MQSVALYTLGCKLNFSESSTLGRKMQDAGYTVKGFHEAADIYVI
                     NTCSVTDHADKKCKKVVKQALRQSPQARIVIVGCYAQLKPQEIAEIPGVDLVLGAAEK
                     FNLVEHIASLPEKQSSGSGAVMAGKVEDVAGFHAAWSFGDRTRTFLKVQDGCDYSCSF
                     CTIPLARGASRSNIISRVVEQANTIANQGVSEVVLTGVNLGDFGLRNATENNESFYEL
                     ICSLEDTNIQRFRISSIEPNLLHEQIIKQVSNSKKFVPHFHIPLQSGSNKILRLMRRR
                     YVRELYAERVGMIKSLMPDCCIGVDVITGFPGETHEDFIDTFQFLQTLDISYLHVFTY
                     SERPNTVAIDLPDSVPQQIREERTAQLRNLSEKKRRAFYEQHQHTTRPVLFEEETDGQ
                     WMFGFTDNYIRVKTQYDPLLVNEIVHVALGEIDAEGHMQAIAIDQTENSHQFHHLQAP
                     EL"
     gene            6098..7399
                     /locus_tag="PLM90_07195"
     CDS             6098..7399
                     /locus_tag="PLM90_07195"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ89194.1"
                     /translation="MILLRSYANLGEFFNAVFGTNMRLPVFSFGFFVGLAFMAAGYIL
                     YRELRRKEKAGIFHAEKVKVMVGEPASAFELISNAVFGFILGFKIGGMIFNWQTFNDD
                     PQSFMFSGEGNILVGLLGAALLAYSKYREKKKEQLPQPQEKTLLLYPSARLGDFVMLA
                     AIFGILGAKMFSNFEEENGWSEFLQNPFKNFFSGLTIYGGLILGAAAIIYYAIRKKIN
                     VLHLGDAVAPALILAYGIGRIGCQVSGDGDWGIINSAYISQENGTLREAEKGEIDSVI
                     QIHAAYYIREFDSLSNVKSVYVKAPGFLPRSLVAQNYAYNVNKEGMYIAKCQGDYCGQ
                     LPASVFPTPIYETIMSLMIFGMLWWLRRRVTVPGMILAIYVIFVGIERFFIEKIRVNN
                     VLEFAGIRATQATFISIIFVIFGLVMALVLVQVEKRRRMSH"
     gene            7552..9396
                     /locus_tag="PLM90_07200"
     CDS             7552..9396
                     /locus_tag="PLM90_07200"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMZ89195.1"
                     /translation="MKTVLRNLLCALSVLPFLTLPLNTYAQLNVDDTYTAEELVSTLL
                     GEGVTVSDITIDCPGGAYGYFDCVDCNVGIPNGILLTSGSVGNAVGPNNSPSQSSSLG
                     MPGDPDLDMIPGVLGTNDACVLEFDFTATSDTIRFNYSFGSEEYLEYVGSFNDVFAFY
                     ISGPGIAGTDNMALIPGTATTVSINNVNDAVNSEYYNINGTGFNAPFNSDDYYIQYDG
                     FTTVLEAKHAVFPCETYHLKMAIADDLDDALDSGVFIEAGSLTSPGVTVEYNTDIEGY
                     PYIIEGCNEGMITFSLSFAPVDTFTVTLNVDGTALMGSDYTTFNNVLEFLPGDTLIEI
                     PIVAEEDGIDEGVETIIITLDLGCVAGVGDSLVVLVYDALPLLVSADTLICPGTEATL
                     TASGADSYSWSPAETVTDPTEATTTVFPDENTTYTVTGTLASCVNTAETTVEIQEPTA
                     DAGADTTIYLGEFASLDADGGVEYSWSPSTGLSETDIENPTASPDFTTTYTVTVTTAI
                     GCEFTDQVTVTVSSDAMVGVPTAFSPNSDGVNDGFTIIVRGQVATWLLQVYNRWGQLV
                     FETDNYAAAWNGTFEGSEQPLGSYVYVLNYKDMNGESFVQQGNFTLVR"
     gene            9573..10589
                     /locus_tag="PLM90_07205"
     CDS             9573..10589
                     /locus_tag="PLM90_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ89196.1"
                     /translation="MMKLYKIACLAACMAVGVLQAQDIHFSQYNASPLALNPALTGVN
                     SCDWRIGATYRNQWNSVTTPYVTYEVFGDMPLIKDIGGASKLAGGLLLYNDVSGDGDL
                     TNLSAMGNVSFVLGLGGQTNHNLSIGVQAGLMHKSLDFNALKWPNQWDGDEFNTAIDP
                     LENIGENISRFNMGAGIAYHNSVSKTFNITGGVGLFNLISPKETFLQDEENKLGMRIS
                     GHLKAGITIDGKYGIIPSVLYQSQSGAKEIIVGSDFGYFLNSANFPATFFLGGAYRLD
                     DAVIASVGMDYKNFRFGASYDINTSTLQDASGGKGGFEISLVYTGCILPVIPKNYVMP
                     CPRY"
     gene            10639..13293
                     /locus_tag="PLM90_07210"
     CDS             10639..13293
                     /locus_tag="PLM90_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ89197.1"
                     /translation="MKKIFAILTIVLFASKAFAQPVDPDVDDMNVTRKLMYGDALMSA
                     GSYYNAQDVYKKAYDDEASEESAYKLARAYYLARNYKEAEKWYKTAMDAGKGKGGAYP
                     EAGFYYAMSLKYNGKYAEAKEAFKAVSKNSNLKGTTGTALKRQAKNEDKGCDLALELG
                     KKPADVNVEHLGSNVNNNYTDLSPRYNPDGDLTYASLVSEDVIDMGSQKKTELKARIF
                     ESKMEGESWAKSKEMSAPFNKGAAHTGNGAYSLDNKRFYYTECNPNDSLVMVCKIFVS
                     EFEAGEWSNGKEVSQVNESGFTSTHPTVGTFKGKEYLYFSSNRTGGRGGMDIWYSEIQ
                     NKGKSYSNPMNCGTKVNTFGDEITPFYNATTETFYFSSNGQINIGGFDIYKTKGGAKT
                     FEAPMNMGLPFNSSVDDFYFATDATGKKGFFVSNRKGGFSVKSETCCDDIYAYKYPPE
                     FVIMARAIDMNTNEPIYGASVDLNQSGVKRDSSVTKTANTYEDFYVGTAQDKFEIVAH
                     KEKYKNGIATTSTVGLKESDTLFVDIMMEPIIEMKPIEVKNIYYTTGTWNLRPESFKA
                     LDSLYNILQLSPQLQVEIGSHTDSRDTEEKNRILSEHRADTVRSYLISRGINPDRLIA
                     KGYGETTPKTITSEFKVPGGTTIPNGTVLTEAFINKFKGDDYEYLHQLNRRTEFTIVG
                     VIPNAIITYDEATIEANEARKREEEEKRNKRLQDLQQVDETDTPDTGGTETPKTGTTG
                     TKGNKGTTTTTTAASTLDPTMTKKGNMFEGNAAVNGKNQTKYILNMSPSITIAMVGKD
                     YFIQLWESGAITAADIKNDKPTDLGNGTTVKGDVFTIKTLQLGDITLTNVDTKINLTS
                     TQNLIVGVKVAEKGGCEFDTTKMKFKCK"
     gene            complement(13372..13779)
                     /locus_tag="PLM90_07215"
     CDS             complement(13372..13779)
                     /locus_tag="PLM90_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HMZ89198.1"
                     /translation="MNDKSIIRIAAWSGALSVALGAFGAHGLESMAESGKIDNADLHT
                     FETAVRYQFYHTFALMAVGLLHNYLSEKSMRRIAWLFIAGMLIFSGSLYLLTFSPVLF
                     GERMGWFGAITPLGGLSFIAGWIMLALQFRKSR"
     gene            13856..>15088
                     /locus_tag="PLM90_07220"
     CDS             13856..>15088
                     /locus_tag="PLM90_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89199.1"
                     /translation="MRKRIIIISSIATVIILLTLGWYFFIHKRYSGVMNAMPDDVICF
                     FDINKSESFNEFIQHDPAMQSLADLQLFGKLYNDYALYRAILASDPDLLADITNGHGL
                     AGAFSTGEGRVDYLFLLDLNNAKRFKSKALLPELNGQKPTVRTHTFEKETVYELHYAD
                     LNITLSFVVTNGMLIYSTASVLVENSLHQLKSGDPVSEQSSFSKVKQELPDNQAYTLY
                     IHLPRFGEFLASFTGNQQYASVMQVAKFADWIGCTLTNTEKGISLNGFCAADNATISR
                     YSGAFSSDMHPVVPANTAVLYRIQTEQLAERLSAGMTGEEVNRSFFDNWSGWMGSQLI
                     IGISESLDKNYMRRAFAIIPAQDKQLAVSKINKISVQDTALYRNHPIFAMAQGDVLQA
                     ILHMPVPDTCFATWEEGDL"
BASE COUNT         4476 a         3098 c         3222 g         4292 t
ORIGIN      
        1 acaaaataca tccctgcagg caattcgtta atattgatgg taaagtattg taatccgtga
       61 acttcgcttg atagcattac tttgcctgaa atatcaaata tcttgatatt gccattagtt
      121 acttctccca gagcaatagt tacttgattt gttgcaggat tcggatacaa ccttatctca
      181 ccttcatctt gtgtggcgtt attgattgaa acttcgcaag cagcatttac catatctgta
      241 gcgccttcat ctttataacg ttgcatgtat tcctgataga ataagtttgc tacagtggca
      301 tcatgtataa caatggtgtt ctcgtcgttg cgtgagttgg cactggtgct ccagttgtgt
      361 gagcctgtcc ataccattgg gtctgactga ggacaatttg gatctataat cagatattta
      421 tggtgcaaca aattggtgcc ggaatatttt tcaaaacgat cgcccatagc atcctcacaa
      481 acattaattg ccgtgctgtc atcagcattc gtttcatccc aaataccact ggcaaaaaca
      541 ccgcgatcta atacagcatc ttcgattgcg taactgatgc caaagcgagt gaatgaataa
      601 attgcaaaat gcatatcata atcagccgta ccaatacgct cgatcatgcg tgtttcaact
      661 tcatcacttg gcgagaaatg cacttcaaca cgctttccgc ctatcataaa ttcatgtggc
      721 gtattatttg atttgtcagg accaaatttg cctccaaaca tttcatcaaa ttccagtcga
      781 taagcttttg ctaagctcat atcctgaatc gcaagataat tttgcttatc cattttaatt
      841 tgctgatctg taaagttcat tgaccctgtc agcacccagg cgtcatcagg atcaactgaa
      901 atcccatcaa caacaatgaa cttattatgc ataataccat ataatgcacc atcggcattt
      961 gttgatccat ctggcggtga ttttttcttt ttcccaacgc caacatttat caaaccataa
     1021 ttgaaatcat caacgccatt gtcgcagatt actcgcacat caacaccgcg agcataagcg
     1081 tcgtttatac tgttgatgat atagttgaca ttgtcaatat tgtaaatggc tatgtcgagt
     1141 gaatatttag catgatcgag ataccaaatt acagtgtcat ccatcgcact atttacaaaa
     1201 gtggcattag tccctgttga aacactatta tctacaggat tattgaacca taccttaatt
     1261 tgtcccgacg aattacttgc cgtcgccatt gccgtaattg cagaatacga agtatcatta
     1321 cttgcactta cacttgcagc ttttacatag taaacagttc ctgctgttaa gccggtgagg
     1381 tttaatgcat gcgttgttac catggcagct gaggatgctg tagatccgag cgacatcgtt
     1441 gtgccatagt aaataatggt attgccttca tacaatgtgt tgaaattcac cgtaaaagat
     1501 gaggtagtaa tatctgtttg agtgagtact gaagaaatta ccggcggatt gccacctgtt
     1561 gaaatatcag cgagatcgcg aggcaataat tgataacctc ctgttccacc gggtgcatat
     1621 tgactcatga tgcctgtaat aaatacaggc tcagccggaa ttggcgtgcc atcgatattg
     1681 gtggtagtta ctacgcgaat ctgtgacgtg cctgcagcat cggtaacatc ataatttgtt
     1741 ccacttccag cggtactaaa tgttcctgca tcaacaaaag tggcatttga aatacgcaat
     1801 agtttgcctt cattgccttc tacccatccc gacaatgatg taacatcaat aggtgcaggc
     1861 agcgggtggc cggtgctatt gatggttaca ctgcttgcag gtgatatctc cagtaagctg
     1921 ttatagttga acaatgggcc ggtagcggta atactgtcgc ctctttgaac accggtaaca
     1981 agggtggcgc tgtagacggc gatatttccg gttgcgtcct gtatataacg gatgcttccc
     2041 agctcaggac cgttgagggc aatgccttta acggtaactg atgtgcctac aggttgcaac
     2101 cttgcatctg caatggtcat ttgtgcgagg gatacgctga tggtcaaggc gatcagcgtg
     2161 agtgtggata gaagttttct catataaaaa aataaagccg acaaagatac cgaaattgta
     2221 atctcccaaa gggcgggaaa tggtaacaat atgtgaagga tttcttactt taacattggg
     2281 tagaccaccg ggttggatgg ggtggcatcc atgcagaatg gagctatctg caaattcaac
     2341 aggtaaatgc cgtctgcaat ggcatcgggc acgaatatta gttcggttat agtgcagtca
     2401 aggcgcggcg cctccggata gtgccaaaaa gccttatgcg cagccaacgc acccccatct
     2461 tcctcacggt cgagggacgg gagatctatc aaaatgtgtt ttatccctga ttccctgaaa
     2521 gacaatgcgg ccgttgcctc caggtaaggc ggatttgttc cgctgtatgc cttggtagtt
     2581 tttgccgaca catttggcaa tgttcgaatt atcacggcct caatatcttt ttccagaatt
     2641 gggatgatgt tcttcgacaa caaaacagta tctccgtttt caatgatgtc gggagtaaca
     2701 ctgataatct gggcaataaa atgatgattc tttaatacac cttggatatt aaccggcaaa
     2761 ttggagatat gcgcaataca ttcagtatgt gtgccattgc catgagggtt cacttggaca
     2821 ttcatataat tcaccggact gccctgattg atgtcgccga tgaaagtgcc cataacaacg
     2881 ggtgttacca gaaacggggg tgcatagtag caatttacct gttcgcctgt tgaataggga
     2941 atggcgatgg atcttgaatt tgaaagatcg aattgccttg ctccatgctg aagtatcatt
     3001 tttattgttt tataaattga gtcctgtaca gatcgccatt caattcaatt gttaaaatat
     3061 agaaacctga aggtagcatg cgaatatcga tttcatttgc tgaattttct aatcgaagcg
     3121 tttttccgct taaatccatc gcgtatatct ctgcacgtac atcaatctga attcctgaaa
     3181 tcatgagcgt atgtgatgcc ggacatgggt atacagataa tacactttca atatccactt
     3241 ccgattgcct gcatgctgcg ccatcagtgg taacggtaag cacgggactg ccatacactg
     3301 agcctccgca gaatgcacga acttttactt tataggtatg acaaggcagc atctcatcga
     3361 gatatccaat aggggcgtag acagtttttg aaatggttgg tgatggagcc gggcaatcaa
     3421 tgcatttagc gaataattta taataatctg cataggtgtc actccacgtt gcctggagtc
     3481 cgagtgaatc ggtataaaca tttaatactt caacaggggc acatggtaac ggtgttgaac
     3541 catttaattc ccaaaacatc acatccttta ttggtccaac ctcacaattt tcagacaaga
     3601 tataatccgt gtcaccatct ctgtccagat cgccataagt aagtgcttca tttccgcata
     3661 tcggtacaat tgaatccatt aatgtaaacg taaaatctgc atttccaagt aaattgatta
     3721 catggtaatt tccggttatt acccatttac caaacatgtc aggaaaggaa tcaccattga
     3781 tatccccaag tccaataaga attgcaccgc cgggagggtc atatgctaca gatgtggtga
     3841 aattttcgga accatcattt tcatattgct ttatgatgta gtctgaaaca ctgataatat
     3901 ccttgtcgcc atcaagatcc atatcctcaa taaaaatttt attaaagaaa atatcacctg
     3961 aatttattac gagatgtcgc gtgtaaccgg ctacaatttt ttcataccaa taaacacgat
     4021 tagcagtgcc taatacaaaa tccatatcac cgtcatcatc aagatcaccg gcaccgaaag
     4081 actccggcgt gttgatattg tcaatgatat gactggtcac atttccatca ccgtcgtttt
     4141 caagccatgt tacatcaaac attacacctc caatacttgg tgctatcctt aaaatgtcag
     4201 gatctagatc gccatcaagg tctgaaaccg caaaatttcg atagtaccct gataaatatg
     4261 tcaattcgtg aggcacaaaa tctccatcgc catgattttc ataaaaatat aagaccgtgc
     4321 catcaagttg gataggtgcc agaatatcca tgtctccatc catatcaaaa tcagtagtgt
     4381 gaatgtaata tccatattca gttgcggcga tatcatgttg cacgaatggc ggtgttccct
     4441 cattctccaa ccatgctaaa acggttgggt tgccatctct ggctatataa cctaccaggt
     4501 ccatatcgcc atcactgtcg atatcggcca tatctcggat ataaggccga tcgaggtagg
     4561 cggcgcctag ggcgtaaagt gaactggttg tgaagggcag ttgagcatgg gaatccatta
     4621 aaatacccaa aaacgctata aggggaatta tacgtttcat agagagattt tactcaaata
     4681 tattaaattt tataaaatgg atgctaaggg tagaaatcag taactttgtc ccccttgtca
     4741 caatgcagtc cgtagcctta tataccttgg gttgtaaact caacttttct gaatcgtcta
     4801 ctttgggtag gaagatgcag gatgccggtt atacagtaaa aggatttcat gaggctgccg
     4861 atatttatgt catcaacact tgttcggtta ccgatcatgc tgataaaaaa tgtaaaaagg
     4921 tagtaaaaca agcgctacgt cagtcacctc aggcgcgtat tgtcatagtc ggatgttatg
     4981 cccaactcaa accgcaggaa attgccgaaa ttccgggtgt tgacttggta ttaggtgcgg
     5041 cagaaaaatt taacctggta gagcatattg cctcattacc cgaaaagcaa tcaagcggca
     5101 gcggcgcagt aatggcagga aaagtggaag acgtggccgg atttcacgct gcatggtcgt
     5161 ttggcgatcg cacgcgtact tttttgaaag tacaagatgg atgtgactat tcgtgctcat
     5221 tttgtactat cccattagca cggggtgcca gcagaagcaa tatcatcagc agggtagtgg
     5281 agcaggcaaa tacgatagcg aatcaaggtg tttcggaagt ggtgctgaca ggtgtgaatc
     5341 ttggcgattt tgggctgcgt aacgcaacag aaaacaacga gtcgttttat gagttgattt
     5401 gtagtttgga agataccaat atacaacgat tcagaatttc atctatcgaa ccgaatttat
     5461 tgcacgaaca gattattaaa caagtatcga attcgaaaaa atttgttccg cattttcata
     5521 ttccactgca aagcgggagc aataaaattt tgcgattgat gcgcagaaga tatgtgcgtg
     5581 aactatatgc cgaacgcgtt ggcatgatca aatcactcat gcctgattgt tgcattggtg
     5641 ttgatgtaat aacagggttt cccggcgaaa cacacgaaga ttttattgat acctttcaat
     5701 tccttcaaac actcgatatt agttatttac atgtctttac ctacagtgaa cgtcctaata
     5761 cggtagctat tgatttacct gatagtgtgc ctcaacaaat tcgcgaagaa cgcactgcac
     5821 agttgcgcaa tttgagtgaa aaaaaacgac gtgcattcta tgagcaacat caacatacaa
     5881 cccgacctgt tttgtttgaa gaagagaccg acgggcagtg gatgtttggg tttacagaca
     5941 actatattcg tgttaaaaca caatatgatc cattgctggt aaatgaaata gtacatgttg
     6001 cacttggcga aattgacgct gaaggacaca tgcaggcaat agcaatagat caaactgaaa
     6061 atagtcatca atttcatcac cttcaagctc ctgaattatg atattactgc gttcctatgc
     6121 caaccttggt gaattcttta atgcagtgtt cggaaccaat atgcggttgc cggtttttag
     6181 cttcggattc tttgtgggac tggcgttcat ggcagcaggc tatatcctat acagggaact
     6241 cagacgcaag gaaaaggcag ggatttttca cgctgaaaag gtgaaagtaa tggttggaga
     6301 accggcatct gcttttgagt tgatcagcaa tgcggtgttc ggatttatcc tcggatttaa
     6361 aatcggaggc atgattttta attggcaaac atttaatgat gatccgcaaa gttttatgtt
     6421 tagtggtgaa gggaatatcc tggtaggatt gctcggcgcc gctttattag catacagtaa
     6481 atacagggaa aaaaagaagg aacaattacc tcaaccacag gaaaaaacat tactgctata
     6541 tccatctgca cgactcggtg attttgtgat gttggctgca atttttggaa tccttggagc
     6601 gaaaatgttt tctaattttg aagaagaaaa cggctggtct gagtttttgc aaaatccttt
     6661 taaaaatttc ttcagcgggc tgactattta cggcggactt attcttggtg cggctgcgat
     6721 tatttattat gcaattcgaa aaaaaataaa tgttttgcac cttggcgacg ctgttgctcc
     6781 tgcattaatt ctggcttacg gaattggtcg cattggatgt caggtttcag gtgatggcga
     6841 ttggggaata atcaattcgg catatatctc acaggaaaat ggcacccttc gtgaagccga
     6901 aaaaggtgaa attgattctg taatccaaat acacgctgca tactacattc gagaatttga
     6961 ttcattatct aatgtgaaat cggtttatgt taaagctccc ggttttttac cacgcagcct
     7021 cgtagcacag aattatgcct ataatgtcaa taaagagggc atgtacattg ctaaatgtca
     7081 gggcgactat tgcggacaac ttccggcatc tgtttttccg actccgatat atgaaacgat
     7141 tatgtcacta atgatcttcg gcatgctgtg gtggctccgt cgccgcgtaa ccgttccggg
     7201 tatgattttg gccatttatg tcatttttgt tggcatagaa aggtttttta tcgaaaaaat
     7261 cagggtcaat aatgtgctgg agtttgccgg tatcagggct acccaggcaa ccttcatttc
     7321 aataattttc gtaattttcg gacttgttat ggctttggtg ctcgttcaag tagagaaaag
     7381 acgtcgtatg agccattgac atcaagctcc ttcactcaca cacagcattt tgccaggtca
     7441 tgatccggta aaaatcattg gcatgagctt atttgttcaa gcttttaggt tgaatattat
     7501 tttgtttgta agtttgattg tattttagtg tcacatttaa attagttttt gatgaaaact
     7561 gttctacgca acctcctctg tgcgttgtcg gtgttgcctt tccttaccct cccgctcaac
     7621 acctatgcgc aattgaatgt tgatgatact tatactgccg aagagcttgt ttctacgctt
     7681 ttgggtgaag gtgttacggt gtctgacatc actatcgact gccccggcgg cgcttacggt
     7741 tactttgatt gtgttgattg taatgtaggc atacccaacg gtatcctgct taccagcgga
     7801 agtgtcggaa atgcagttgg accaaacaac tcaccaagcc aatcgagcag cctcggaatg
     7861 cccggtgatc ctgatttgga tatgattccg ggggtgttgg gaactaatga cgcctgcgtt
     7921 ctggaattcg atttcaccgc aacgtcagat accattcgtt tcaactattc ctttggatct
     7981 gaagaatacc tggaatacgt tggttctttc aacgatgtat ttgccttcta tatcagcggt
     8041 cccggtattg caggaactga taatatggcc ttgattccgg gcacggctac aaccgtgagt
     8101 attaataatg tgaacgatgc cgtgaacagc gaatattata atattaatgg caccggattt
     8161 aatgctcctt ttaactcaga tgactattac atacaatacg atggatttac aaccgttttg
     8221 gaagcaaaac acgctgtatt cccttgtgaa acatatcacc tgaaaatggc cattgccgat
     8281 gacttagatg atgccctaga ctcaggtgta ttcattgaag caggctcact taccagcccg
     8341 ggtgttaccg tagaatataa tactgatatc gaaggttacc cttatatcat tgaaggctgt
     8401 aatgaaggta tgatcacttt cagcctgtca tttgcaccgg ttgatacctt taccgtaaca
     8461 ttgaacgttg atggaactgc cctgatgggg agtgattata ccactttcaa caacgtgttg
     8521 gaattcttac ccggcgatac cctcatcgaa atacctattg tagctgaaga agatggcata
     8581 gacgaagggg ttgaaactat tatcatcaca ctcgacctgg gttgcgttgc aggtgttggc
     8641 gacagtcttg tagttctggt ctacgatgcc ctgccattgc tggtttcagc cgatacattg
     8701 atctgtccgg gaacagaagc cacgctgaca gcttccggtg ccgacagcta ttcatggtcg
     8761 cctgctgaaa cagttaccga cccgactgaa gctactacta cggttttccc ggatgagaat
     8821 accacttata ccgttacagg aacactggcc tcatgtgtaa ataccgctga aactactgta
     8881 gagatacaag aaccgacagc tgatgcaggc gcggatacta ctatatactt aggggaattt
     8941 gccagccttg acgctgatgg cggggtggag tacagctggt cgccttctac aggtttgagt
     9001 gaaacagata ttgaaaaccc aactgcttca cctgacttta ccacaacgta tacagttacg
     9061 gttactacag ctataggatg tgagtttacc gatcaagtga cggttacagt aagctctgat
     9121 gctatggtag gtgttcccac agcgttttca ccaaattctg atggtgtgaa cgatggattt
     9181 accattattg tgagaggtca ggtggctacc tggttattgc aggtttacaa ccgatggggg
     9241 caactggttt ttgaaaccga taattatgca gcagcatgga atggcacttt tgagggctca
     9301 gaacagcctt tgggaagcta tgtatatgtg ctgaattata aggatatgaa tggtgaaagc
     9361 tttgtacaac aaggtaattt caccctggta agataatttg agttccaatt gatcgctaac
     9421 aagccaaaat gcttgcagat taaggacaat ttagtatttt taacccgttg gggtaggggg
     9481 atgtaggcat tagtatacag ccgataactc caaaaacaag caaaaactga taaccaacat
     9541 aacattactc tgaaaatttt aaacaataac caatgatgaa gctttacaaa atcgcgtgtt
     9601 tggcagcttg catggctgtg ggagtgcttc aggctcagga cattcacttt tcacaataca
     9661 acgcctcacc tctggcgttg aaccctgccc tcacaggtgt gaatagttgt gactggcgta
     9721 taggtgctac ttaccgcaac cagtggaaca gcgttactac tccttatgtg acctatgaag
     9781 tgtttggcga catgccactt atcaaagata tcggcggcgc cagcaaactc gccggcggtc
     9841 tgcttttata caacgacgta tccggcgatg gagatttaac caacctctct gcaatgggta
     9901 acgtttcttt tgtattggga cttggtggtc aaacaaatca taacctttct attggtgttc
     9961 aagcaggttt gatgcataaa tcacttgatt ttaatgcact caaatggcca aatcagtggg
    10021 atggtgatga atttaatacg gcaattgatc cactcgaaaa tatcggagaa aatatcagca
    10081 gatttaatat gggggcaggt atagcctacc ataattctgt ttcaaaaaca ttcaacatca
    10141 ccggtggtgt tggtctattc aacttgattt ctcctaagga aacattcctg caagatgaag
    10201 aaaacaaatt gggtatgcgt atctccggtc acctgaaagc tggtattact atcgatggaa
    10261 aatatggtat catcccatca gtattgtatc agtcgcagtc aggtgcaaaa gaaatcattg
    10321 ttggtagtga ttttggatac ttccttaaca gtgcaaactt ccctgcaaca ttcttcctcg
    10381 gtggagcata tcgcctcgac gatgcagtga tcgcatcagt aggtatggat tataaaaact
    10441 tccgcttcgg cgcaagctac gatatcaata cttcaacatt gcaagatgca agcggtggta
    10501 aaggcggttt cgaaatttca ttggtgtata ccggttgtat tttacctgta ataccaaaaa
    10561 attatgtaat gccttgtccg cgttattgat tatcagcaca attaatttga ttactacctc
    10621 cttaaagaga aatagcatat gaaaaaaata ttcgcaattc ttacgatcgt attgtttgca
    10681 tcaaaagcat ttgcgcaacc tgttgatccg gatgtggacg acatgaatgt tacccgcaaa
    10741 ttgatgtatg gcgacgcgtt gatgtcggca ggaagctact ataatgcgca agatgtatac
    10801 aaaaaagcat atgacgatga agcttctgaa gaatctgcct acaagttagc tcgtgcatat
    10861 tacttggctc gcaactataa agaagcagaa aaatggtaca aaactgcaat ggatgccggc
    10921 aaaggaaaag gcggtgccta tcctgaagcg ggattttatt atgccatgag cttgaaatac
    10981 aatggaaaat atgccgaagc taaagaagct ttcaaagccg tttccaaaaa ttcaaacctc
    11041 aaaggaacaa caggaactgc gctgaaacgt caggcaaaaa atgaagataa aggttgcgac
    11101 cttgcacttg agctcggcaa aaaacctgcg gatgtaaatg ttgaacacct tggaagcaat
    11161 gtaaataata attatacaga cctttcacca cgttataacc ctgatggcga tcttacttac
    11221 gcatcattgg tttccgaaga tgtgatagat atgggttcac aaaagaaaac agaactcaaa
    11281 gcgcgcatct tcgaatctaa aatggaaggc gaaagctggg cgaaatcgaa agaaatgtca
    11341 gcacctttta ataagggagc agcacatacc ggaaacggcg cttattcgct cgacaacaaa
    11401 cgtttttact atactgaatg taatcctaac gattcattgg tgatggtatg taaaatcttt
    11461 gtgagtgaat ttgaagcagg cgaatggtca aatggtaaag aagtatcgca ggtaaatgaa
    11521 tcaggattta cttctacgca tcctacagtt ggtactttta aaggaaagga atatctctac
    11581 ttctcatcta atcgcacagg tggtcgcggt ggaatggata tctggtattc agaaattcaa
    11641 aataaaggaa agagctattc aaaccctatg aactgcggaa ccaaagtgaa tacttttgga
    11701 gatgagatta ccccattcta taatgctact accgaaactt tctatttcag ttctaacgga
    11761 caaatcaaca tcggtggttt cgatatctat aaaacaaaag gtggtgctaa aacttttgaa
    11821 gcgcctatga acatgggctt gccattcaat agctctgttg atgatttcta cttcgcaaca
    11881 gacgctacag gcaaaaaagg atttttcgta tcaaaccgca aaggtggttt ctctgtaaaa
    11941 agtgaaactt gctgcgatga tatctatgca tataaatatc cgccggaatt tgtaatcatg
    12001 gctcgagcta tcgacatgaa tacgaatgaa cctatttatg gtgcgtccgt agacttaaac
    12061 caatcaggtg ttaaacgcga tagctctgtt accaaaacag caaatacata tgaagatttt
    12121 tatgttggaa ctgctcaaga taaattcgag attgtagcgc acaaagaaaa atataaaaac
    12181 ggtattgcta caacaagcac tgttggtttg aaagaaagtg ataccctctt tgttgatatt
    12241 atgatggaac caattataga aatgaaacca attgaggtta aaaatattta ctatacaacg
    12301 ggtacttgga accttcgtcc tgaatcattt aaagcactcg attcattata taatatcctt
    12361 cagttgagtc cgcaattaca agttgaaatt ggttcgcaca ctgactctcg cgatacggaa
    12421 gagaaaaacc gcatcctttc agaacatcgc gcagatactg ttaggtctta cctgatatct
    12481 cgcggtatca accctgaccg actgattgct aaaggatatg gtgaaacaac accaaaaacc
    12541 atcactagtg aatttaaagt tccaggcgga acaacgattc caaacggaac tgtattaact
    12601 gaagccttca tcaataaatt caaaggtgat gactatgaat atctgcatca gcttaatcgc
    12661 cgcacagagt tcactatcgt aggtgtaatt ccaaacgcta tcatcactta tgatgaagca
    12721 acgatagaag cgaacgaagc aagaaagcgt gaagaggaag aaaaacgtaa caagcgtctt
    12781 caagaccttc agcaagttga tgaaacagat actcctgata caggtggcac tgaaactcct
    12841 aaaactggaa ctacaggcac aaaaggtaat aaaggaacta ctaccacaac aactgcggct
    12901 tcaactttag atccaacaat gactaaaaag ggtaatatgt ttgaaggaaa tgctgctgtt
    12961 aacggcaaaa atcaaactaa gtatatcctc aacatgagtc caagcatcac cattgccatg
    13021 gttggaaaag attactttat tcaactttgg gaatcaggtg ccatcactgc tgcagatatt
    13081 aaaaatgata aaccaacaga cctcggaaac ggcacaaccg taaaaggtga tgtgtttacc
    13141 attaaaacct tgcagttagg tgatattaca ttgacaaacg ttgatacaaa aatcaatctt
    13201 accagtactc aaaacctcat agtaggtgta aaagttgctg aaaaaggtgg atgcgaattt
    13261 gatacaacta agatgaaatt taagtgcaaa taacagcata accagctata ttgaaaaagg
    13321 ccgtccgctc aggatggcct tttttctttt aggatgtacc tcgaaatatg tctaccggga
    13381 tttgcgaaat tgcaaggcaa gcatgatcca cccggcaata aaactcaatc caccaagtgg
    13441 tgtgatagcg ccaaaccaac ccattctctc gccaaagaga accggtgaaa atgtgagaag
    13501 atataaactc cctgaaaaaa taagcattcc tgcaatgaac agccatgcaa tcctacgcat
    13561 cgatttctcc gagaggtaat tgtgtagcaa ccctaccgcc atcaaggcaa aagtgtgata
    13621 aaactgatag cgcactgcag tttcgaatgt gtgcaagtcg gcattgtcga ttttgcccga
    13681 ttcagccata gattcgagtc catgtgctcc gaatgcgccc aatgctacag ataacgcgcc
    13741 gctccaggct gctatccgaa tgatgctttt atcgttcata ttttatcatg atgtgtaaag
    13801 gtgaataggc tgcgggtatt tgttacccta cctttgcaaa ggtaatcaag acctcatgcg
    13861 caaacgaatc attatcatct catccatcgc cacggtgatc atcctcctca ccttaggctg
    13921 gtattttttt attcataaac gctatagcgg cgtaatgaat gctatgcctg atgacgtgat
    13981 atgttttttc gatatcaata aatcagaatc atttaatgaa tttattcagc atgacccggc
    14041 tatgcaatca cttgctgatt tgcaattgtt tggaaagctc tataacgatt atgcccttta
    14101 tcgcgccatt cttgcaagtg atcccgattt gctagctgat attacaaacg gacatggatt
    14161 ggcaggtgca ttttctaccg gtgaaggtcg tgtcgattat ttatttctgc tcgatcttaa
    14221 caacgccaaa cgatttaaat ccaaagcgtt attgccggaa ctcaatgggc aaaagccaac
    14281 agtcagaaca catacttttg aaaaagaaac agtgtatgaa ttgcactatg cagatttgaa
    14341 tatcacacta tcttttgtgg taacaaacgg catgctcatt tatagtacgg catctgtttt
    14401 ggtagaaaat tctttgcatc aattaaaaag tggtgacccg gtaagcgaac aatcatcatt
    14461 tagtaaggtg aagcaggaac ttcccgataa tcaggcctac accttgtaca tacacctgcc
    14521 acgctttgga gaatttttag cttcattcac aggtaatcag caatatgcct cggttatgca
    14581 ggttgcgaaa tttgccgatt ggataggatg tacactcacc aatactgaaa aaggaatttc
    14641 attaaatgga ttctgtgctg cagataatgc tacaatctcc cggtattcag gtgcatttag
    14701 tagcgatatg catcccgttg ttcctgcaaa cacggctgtg ttgtatagaa ttcaaactga
    14761 gcaactggca gagcgcttat ccgctggcat gaccggcgaa gaagttaatc gttcgttttt
    14821 tgataattgg tcgggatgga tgggttcaca attgatcatc ggcatatcag aatcattgga
    14881 caaaaattac atgcgaaggg catttgctat catccccgcg caagacaagc aattggcagt
    14941 ttcgaaaata aataaaatca gtgtacagga cacagcacta tatcgcaatc atcccatttt
    15001 tgcaatggca caaggcgatg tgttgcaagc catcctgcat atgcccgttc ccgatacttg
    15061 ctttgcaact tgggaggaag gtgacctt
//
LOCUS       DAOQOU010000064        14999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2024_length_14999_cov_6.862286, whole genome shotgun sequence.
ACCESSION   DAOQOU010000064 DAOQOU010000000
VERSION     DAOQOU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2024_length_14999_cov_6.862286"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1492
                     /locus_tag="PLM90_07225"
     CDS             233..1492
                     /locus_tag="PLM90_07225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HMZ89200.1"
                     /translation="MTSRNIIILGAGSAGTMMANHLRGKLDRNEWNITIVDEVEIHYY
                     QPGFLFLPFGIYDEHEIQKPIKKFIPHDVQLLHTTIDRVEPETNLVHMTDGSALHYDI
                     LIIASGCNIAPEEVEGMKGELWYQDIFDFYSFKGAKALHEKLANWEGGKLVIHITEMP
                     IKCPVAPLEFAFLADDFFRHKGMRNEVEITYVTPLSGAFTKPFASKKLGHLIEDKGIK
                     LVTDFCVERIDNKTKTLHDYGGQEVNFDLLVTVPLNKGAEYVGRSGFGDDLNYVPTDK
                     FTLQTTIAPNIFAIGDATSLPASKAGSVAHFESEVLTQNILDFINDDPLSAKFDGHAN
                     CFVETGNHKALLIDFNYDHEPVDGTFPFNGVGPMQLMEESLMNHMGKLGFKWIYWNVL
                     MKGRTIPFIHPTMDTSGKHLEHETLNK"
     gene            1512..1823
                     /locus_tag="PLM90_07230"
     CDS             1512..1823
                     /locus_tag="PLM90_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HMZ89201.1"
                     /translation="MTTLVNEKNVQFNEEGFMTDFSQWDKEVAEMIAAKEGIPVLTPK
                     HWEVINWLQEQHKSKNPLTIRKVGGSGITDIKEFYALFPNGPLKKAAKISGIPKPVSC
                     I"
     gene            1846..2367
                     /locus_tag="PLM90_07235"
     CDS             1846..2367
                     /locus_tag="PLM90_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HMZ89202.1"
                     /translation="MEDLLLNDAPVINEVDQKEPVRKMNFILSKAGLDSVYACFIMAN
                     GARMEGIEAEIFFTFFGLDAVNKKRMEHLHVATVGNPGMHMPTVLGAIPGMETFATNM
                     MKKQMEDLDLPTVPEFLEILEASGVEMYGCKLAADMFKLKKEDLFEGIKDIITVGDFY
                     AKASGKGSHIVFI"
     gene            complement(2480..3340)
                     /locus_tag="PLM90_07240"
     CDS             complement(2480..3340)
                     /locus_tag="PLM90_07240"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMZ89203.1"
                     /translation="MRIDPSELTIPKLHQYILGSVSPRPISFASTVDLEGRPNLAPFS
                     FFTAVGSNPPMVVFSPARSGRDNTTKHTLDNVEATKEVVINIVNYPIVHQMSLASSPY
                     PKGVNEFIKSGLTPIPSEKVKPFRVKESIVSMECIVRDVIHTGDKGGAGNIVMCEIVL
                     MHIDDNILDADGRMDPFKMDLVARMGGEYYARIIPESIFVLPQPKTEVGIGVDSLPEF
                     ARNSHVLSGNDLGKLGTLQELPNEEALASAKTYLQAGMTGEDACVVAKQLISEGKTTE
                     ALALLVSFSE"
     gene            complement(3379..4659)
                     /gene="fahA"
                     /locus_tag="PLM90_07245"
     CDS             complement(3379..4659)
                     /gene="fahA"
                     /locus_tag="PLM90_07245"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HMZ89204.1"
                     /translation="MKIAANDPKRKSWVPIPDGSDFPIQNLPFGIFVNDGKPAHVGVA
                     IGEYVMDLHALFRAGFLEEFDLTESMLENRFLNDLLHIGKKKMRALRDRISDLLLDSN
                     ESLQQHQEVYGSILIPMDQVEMLLPIQPGDYTDFYSSIVHATNVGSMFRPDNPLLPNW
                     KHIPIGYHGRSSSMIVSGTSFHRPLGQTIVKDGDPPIFGPTKQLDFELEMGFITFDGK
                     PLGQRISIEEADDYIFGMVIFNDWSARDIQRWEYVPLGPFLSKNFASSLSPWVVTLDA
                     LEPFRIAGEKQEPAVLPYLQYHGEKNLDINLQVFIKPDGSEAQLVSASNYKHMYWTME
                     QQLAHQTVNGCNVRAGDLYASGTISAPEATGFGSMLELAWRGTKPINMHDGTTRSFIL
                     DGDTVTIKAWSEKNGLRIGFGEVTAKVLPAIPFE"
     gene            4720..5202
                     /locus_tag="PLM90_07250"
     CDS             4720..5202
                     /locus_tag="PLM90_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HMZ89205.1"
                     /translation="MDIALLIIACVLMLGGIAGSFLPILPGPPLCFAGFLVVQFSIYA
                     SFSTEFLIIWAVVVVILGILEYYIPTWGTKKFGGTKSGQKGATVGTILGIFIPPQPLW
                     LILGPFVGAYLGELIHDARDNKKALRSAWGSFIGFLAGTFIKVVTVIVMAVFFIREVT
                     "
     gene            complement(5206..5559)
                     /locus_tag="PLM90_07255"
     CDS             complement(5206..5559)
                     /locus_tag="PLM90_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89206.1"
                     /translation="MAESNNWINTTLQEVVSLGELEVSESRLTVKNEDDARTLLIEMI
                     AFLIDHNFEKLLFILYRIDIDEQKVKLLLSKHLPDEAPVVIADLIIERQKQKEAFKAS
                     FEHTPPEAMDEDLLL"
     gene            complement(5546..6403)
                     /locus_tag="PLM90_07260"
     CDS             complement(5546..6403)
                     /locus_tag="PLM90_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HMZ89207.1"
                     /translation="MQKHLTRRTSRTKSQQSLADHLDGLVVKYNNPDFIKGDPISLPH
                     KFTKLQDREIIGFWTAMLSWGQRKTIINKAEELLLLMHNSPYDFILHHKEDDLKRFAD
                     FKHRTFNYTDTLWFIYFLRSFYAEHNSLEEAFLCEGFREEVHVEKMLIHFHNLFFSDR
                     DAPQRTKKHIPTPASKSACKRLNMFLRWMVRKDNAGVDFGCWQQIQPSQLICPLDVHV
                     ERVARKLGLLTRKQTDWDAAVELTENLRMFDHSDPVKYDYALFGSGVMEKHVLDSEKK
                     AIINIHGRK"
     gene            complement(6366..7136)
                     /locus_tag="PLM90_07265"
     CDS             complement(6366..7136)
                     /locus_tag="PLM90_07265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HMZ89208.1"
                     /translation="MDFQTLALLALAVGPAVAIMFFFYSKDKNDREPFGVLLVSFLTG
                     CFSVLPAIILEKALPHVVPGAQGESLISIAIFTFIIVAGSEEFSKYLFLRYYAYRKPS
                     FNEPFDGIVYAIMVGMGFATIENLLYVFGADTLGASWGTAGIRAVTAIPAHATFAAIM
                     GYFIGLAKFNRSHERRLMFYGFIGAVILHGFYDFFLFQNYKEGLVIGALISLIAGIIF
                     SIKAMRLHRANPFAIKPAAAQTKQHSDAETFDSPNKPN"
     gene            complement(7239..8378)
                     /locus_tag="PLM90_07270"
     CDS             complement(7239..8378)
                     /locus_tag="PLM90_07270"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HMZ89209.1"
                     /translation="MKFNTKVIHAGVEPDPSTGAIMTPIFQTSTYVQNAPGDHKGYEY
                     ARTQNPTRHVLQQNLAALENGNFGICFSSGLAATDGIIKMFSEGDHIIANNDLYGGTY
                     RAFTKVYARFGMEFTFADLSDASQLEPLIKSSTKLIWVETPTNPMLRIVDIAAICAIA
                     KKHNILVCVDNTFASPYLQNPLDHGADIVIHSATKYLGGHSDVIHGAVIVKEQSLADQ
                     LYFIQNATGAVPGPQDCFLVLRGIKTLHLRVQRSCENAAVIAHWLKAHPKVDKVYWPG
                     FEDHANHAVAKKQMRMFGAMVSFTLKGDDFEEAKRILSSTHLFSLAESLGGVESLIGH
                     PASMTHASIPKEERLKNGLVDSLIRLSVGVEDVEDLVADLQKALG"
     gene            complement(8603..8965)
                     /locus_tag="PLM90_07275"
     CDS             complement(8603..8965)
                     /locus_tag="PLM90_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ89210.1"
                     /translation="MNLPHSANKSIAVKLTFEFSLDIMHLCSQLDQGYKYSYSKQLFR
                     SGTGIGANVIEAQNAESKKDFLHKIKIAAKEAEETEYWLLLGNQSEEYPDMKDYLDKL
                     YAIKRVLNSIISTTKANL"
     gene            complement(9310..10551)
                     /locus_tag="PLM90_07280"
     CDS             complement(9310..10551)
                     /locus_tag="PLM90_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HMZ89211.1"
                     /translation="MAIIRPFQGWRPQAAQAEQVASRPYDVLNSEEARVEAAGNDLSF
                     LHVCKPEIDLPEEVDHYDDAVYAKAVENWKRFQNNGTFVQDATRNMYAYRQIMNGHAQ
                     VGLVANSAISDYFDDIIKKHEFTRPEKENDRIRHMYDLQCHPEPVFLTYPDVADIDAI
                     MHKVVAGTPIYDFTADDGIQHTFWVISDAQDVAGLTQLFSSKVPFTYIADGHHRSASS
                     AKVGQRMKDANPNHNGEEEYNYFLSVIFPSSQLMIMDYNRVVKDSNGLSEDQLLDRIG
                     EKFSIEKHEGIYKPEGLHHFGLYINNHWYKLTAKQGTYNDNDPIDVLDVTILSNHLLD
                     PILGIKDQRTDKRIDFVGGIRGLGELEKRVNSGEMALAISLYPVSIQQLINIADSGNV
                     MPPKSTWFEPKLRSGLVVHTF"
     gene            10667..11533
                     /locus_tag="PLM90_07285"
     CDS             10667..11533
                     /locus_tag="PLM90_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89212.1"
                     /translation="MTKNYKLVTAQKLAAYSAMAAAITASMSDANAEVIYTDIDDVTI
                     GVGDIYQLDIDADGTADFMFGGGSTMSSGGSTWSFVSAFGYVTSYTVGNADNQIIGYT
                     GSFYNYGSALEDGALIGPDGPWLNYPSLSNSAVLASNFYGSTYGQFPGLGERFMGIKF
                     SSKGNLHYGWIRVVADIDPVYATIMDYAYETVNDVPIEAGSTISLVGVEELSAGTVNV
                     YAFNSSVRVVVNQDLTSPQIRITDLHGATVFTAPLNNRQATIDLKNFSTGNYVVSVLS
                     AEGTSNKQVFIN"
     gene            complement(11614..12534)
                     /locus_tag="PLM90_07290"
     CDS             complement(11614..12534)
                     /locus_tag="PLM90_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ89213.1"
                     /translation="MGTILLATEKPFAAEAVEKIRTVVTGGGHQLALLENYTEKQQLL
                     NAVADADALIVRSDIVDADVINAGNHLKIVVRAGAGYDNLDLKSASANNVVCMNTPGQ
                     NANAVAECAFGMMLFMARKQFSGSSGTELRGKNLGIVGYGNIGRYMAQIGRGFGMDVY
                     AYKRNMDRLAMEIDGVTPASSFEDVFEHCQYISLHLPSTAETKGVINYHLLRLMPKGA
                     MLVNTARVDVVNEDDLLKLMEERSDIQYVSDFIPKQESVFKEKFAKRIFYPAKKMGAQ
                     TEEANVNAGIAAAKQILDFLDKGIDTFRVN"
     gene            complement(12627..13694)
                     /gene="serC"
                     /locus_tag="PLM90_07295"
     CDS             complement(12627..13694)
                     /gene="serC"
                     /locus_tag="PLM90_07295"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HMZ89214.1"
                     /translation="MKVHNFNAGPAILPAPVISQAAQAVTEFNGLGMSLLEISHRSKD
                     FQAVVDEATALVKEIAGLDDSYKVLFLQGGASTQFGMVPMNLLDASETAGYCNTGTWA
                     KKAQKEAKQFGNVLELATSEPDNFTFIPKGYQIPDNLKYFHITSNNTIYGTQYHAYPK
                     SPVPYIIDMSSDIFSRNCDFSQFDLIYAGAQKNIGSSGVTVVILKESLLGKVNRTIPT
                     MMDYRTHIKENSLFNTPPVFSIYVTMLTLRWIKEQGLNIIESVNRSKAELLYNAIDSN
                     PLFKGTVAKEDRSWMNVNFIMENRDLEPEFLAFAKANNCVGLAGHRSVGGMRASLYNA
                     LGLDSVQHLVNLMNEFAAKKG"
     gene            14078..>14999
                     /locus_tag="PLM90_07300"
     rRNA            14078..>14999
                     /locus_tag="PLM90_07300"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4453 a         3268 c         2888 g         4390 t
ORIGIN      
        1 atcaagtatc aagtattttt atcccgacct gcggtcggga tttttttttg cctttgataa
       61 tactcaccca ctgacccact gacccactca cccactcacc cactcaccca ctcacccact
      121 cacccactga cccccctcac caaaacggtg acataaatca atttccgctg tatgaaacat
      181 cacctgttat cgacctccgt cgggaataat tttgtgtcat aaaataaaca caatgacaag
      241 caggaacata atcatcttag gtgccggatc agccggaact atgatggcga accatttgcg
      301 gggaaaactg gatagaaatg agtggaatat caccatcgtt gatgaagttg aaatccacta
      361 ttatcaaccc ggattcttat tcctcccatt tggtatttac gatgaacatg agattcaaaa
      421 accaattaaa aaattcatcc ctcacgatgt gcaattgcta cacactacca tcgatcgcgt
      481 agaacctgaa actaatttag tacatatgac agatggcagc gcgttgcact acgatattct
      541 cattatcgcc tcaggttgca acatcgctcc cgaagaagtt gaaggaatga aaggtgaact
      601 gtggtatcaa gatatttttg atttctacag ctttaaaggt gccaaagcat tgcatgaaaa
      661 attggcaaat tgggaaggtg gcaaattagt gatacacatc actgaaatgc cgattaaatg
      721 ccccgtagca ccactcgaat ttgcgtttct tgccgatgat ttctttcgcc ataaaggaat
      781 gcgaaatgaa gttgaaatca cttatgtgac ccctctcagc ggcgctttca caaaaccatt
      841 tgcttcaaaa aaacttggtc acctgattga agataaaggc attaaactgg ttaccgactt
      901 ctgtgttgag cgcatcgaca ataaaacaaa aactttgcac gattacggcg gacaagaagt
      961 gaatttcgat ttattggtaa ccgtgccatt gaataaaggt gctgaatatg taggtcgaag
     1021 tggttttggc gatgatttga attatgtgcc aacagataaa ttcacactcc agactactat
     1081 cgcaccaaac atttttgcca tcggcgatgc taccagtcta ccggccagta aagccggctc
     1141 agttgcacac tttgagtctg aagtgcttac tcaaaacatc ctcgatttca ttaatgatga
     1201 tccattgagt gcaaaatttg atggtcacgc aaattgcttc gttgaaaccg gtaatcataa
     1261 agcactattg atagatttca attacgacca cgaaccggtt gacggaacat tcccattcaa
     1321 tggtgttggc ccgatgcaat tgatggaaga gtcacttatg aaccacatgg gtaaacttgg
     1381 tttcaaatgg atttattgga atgtgctcat gaaaggacgc actattccat tcattcatcc
     1441 aaccatggat accagcggaa aacacctcga acacgaaact ttaaataaat aacaacatta
     1501 aacataccat tatgacaacg cttgtaaatg aaaaaaatgt tcagttcaat gaagaaggtt
     1561 tcatgactga ttttagccaa tgggataaag aagttgccga aatgattgcc gcaaaagaag
     1621 gcatcccggt tctcactcct aaacattggg aagtaattaa ctggttgcag gaacaacata
     1681 aaagcaaaaa tccattgacc attcgtaaag ttggtggaag tggcataact gatatcaaag
     1741 aattttatgc tttattccca aatggaccac tgaaaaaagc agctaaaatt tccggtatac
     1801 caaaacctgt aagctgtatt taataactgc atttaaaaca ttactatgga agacttactg
     1861 ttaaatgatg ctcctgtgat caacgaggtt gatcaaaagg aacccgttcg taaaatgaac
     1921 ttcatcctgt caaaagcagg tttagatagc gtttacgctt gtttcatcat ggctaacggc
     1981 gctcgtatgg aaggaattga agccgaaatt ttcttcactt tctttggatt ggatgccgtg
     2041 aataaaaaaa gaatggaaca cttacatgtt gctactgtcg gaaatccggg aatgcacatg
     2101 cctacagtac taggcgctat tcccggaatg gaaaccttcg caaccaatat gatgaaaaaa
     2161 caaatggaag accttgatct gccaactgta cctgaattcc tcgaaatatt agaagcttcc
     2221 ggagttgaaa tgtacggctg taaacttgct gccgatatgt ttaaacttaa aaaagaagac
     2281 ctgttcgaag gcatcaaaga tatcattacc gttggcgatt tctacgcaaa agccagcggc
     2341 aagggctcgc acattgtgtt tatttaaaat agtatgtagc tccgaccgaa aggtcggggc
     2401 tctttttttt aattatgtga aacttgtacg ataggtagac acttggtaaa tcggtagcgt
     2461 aagtaatttg ctacaaatct cattccgaaa acgatactaa taatgcaagt gcctctgttg
     2521 tctttccttc cgaaattaat tgtttagcaa caacacaggc atcttctccc gtcatgcctg
     2581 cttgcaaata tgtttttgct gaggctaatg cttcttcgtt tggtaattcc tgtaatgttc
     2641 ccagcttacc taaatcgttg cctgataata catgactgtt tcgtgcaaat tcaggaaggc
     2701 tatcaacgcc aatgcctacc tctgttttag gttgtggtaa cacaaatatt gattccggaa
     2761 ttattcttgc ataatattca cctcccatgc gggctacaag gtccatttta aaaggatcca
     2821 tcctgccgtc agcatccaga atattgtcgt caatgtgcat caacacaatt tcacacataa
     2881 caatatttcc cgcgccaccc ttatcgcccg tatgaatcac atctctaaca atacattcca
     2941 tcgaaacaat gctttcctta accctgaaag gttttacttt ttctgatggt attggcgtta
     3001 atcccgattt aataaattca ttcactccct ttggataagg agaacttgca agactcattt
     3061 gatgaactat cggataattt acgatattga taacaacttc cttagtggct tcaacattat
     3121 ctagtgtgtg tttggtggtg ttgtctctgc cgcttcttgc aggggaaaaa acaaccatag
     3181 gcggattgct ccccacagcg gtaaaaaagc tgaatggtgc aagatttggc cttccttcca
     3241 gatcgacagt gcttgcaaaa gaaattggcc ttggcgaaac agagcctaaa atatattgat
     3301 gcagtttcgg aatagtcaat tccgatgggt caattctcat gaataatgat tattattaaa
     3361 tgatgctatt tattgttgtt attcaaatgg tattgccgga agcactttag cagtaacttc
     3421 accaaatcca atgcgtaaac catttttttc tgaccatgct ttaatggtaa cagtgtcgcc
     3481 atctaaaata aaactacgag ttgtcccatc atgcatgttt attggctttg tgcctctcca
     3541 tgccaattcc agcatactgc caaatcccgt agcctcggga gcactgatgg tgccactggc
     3601 gtaaaggtca cctgcacgca cattgcatcc atttaccgtt tgatgcgcta attgctgctc
     3661 catggtccaa tacatgtgct tataattgga tgcactcact aattgagctt cactgccatc
     3721 aggcttaatg aaaacttgta agttgatatc aaggtttttt tcaccatgat attgcagata
     3781 tggaagtaca gcaggttctt gtttttctcc cgcaatgcga aatggctcta atgcatcaag
     3841 tgtaacaacc caaggcgaca atgagctggc aaaattctta ctcagaaacg gtcccagtgg
     3901 aacatattcc cagcgttgaa tatctcttgc actccaatca ttaaatatca ccataccaaa
     3961 aatgtaatca tccgcctctt caatagaaat ccgctgacct aatggtttac catcaaaggt
     4021 gataaacccc atttccaatt cgaaatctaa ctgctttgtc ggaccgaaaa taggtgggtc
     4081 tccgtctttg acaattgtct gaccaagcgg tcggtgaaat gaagtgccgg aaacaatcat
     4141 ggatgaactt ctgccatgat atccaattgg aatatgtttc cagtttggca ataatggatt
     4201 atcaggcctg aacatgctgc caacatttgt tgcatgcaca atgctcgagt aaaaatcagt
     4261 gtaatctccc ggttgaatcg gtagtaacat ctccacctga tccatgggaa ttaaaataga
     4321 accgtatact tcctgatgtt gctgcagcga ttcattgcta tctaataaca aatctgaaat
     4381 gcgatcgcgc aaggcacgca tttttttctt gcctatatgc agaagatcat ttaagaatcg
     4441 attttccaac atggattcgg taaggtcaaa ttcttcaaga aatcctgcac gaaataatgc
     4501 atgaagatcc attacatact cgccaatggc aacaccaaca tgggcaggtt tgccatcatt
     4561 cacaaaaatg ccaaaaggca aattctgtat gggaaagtcg ctgccatcag gtatcggaac
     4621 ccatgatttt ctttttggat cgtttgctgc aatcttcata ttcattaatt aattttacaa
     4681 agttacaaca atggggtcgc atacttgttg atatagatca tggacatcgc acttcttatt
     4741 attgcctgtg tattgatgct cggtggtatt gccggaagct ttttacccat attgcccggt
     4801 cctccattat gctttgccgg attcttagta gtccaattca gcatctacgc ctcgttttca
     4861 actgaatttc tcatcatttg ggctgtagta gtggttatac ttggtatact cgaatattat
     4921 attccaacct ggggaacgaa aaaatttggc ggaacaaaat caggtcaaaa aggtgctacc
     4981 gttggcacta tccttggcat tttcattcca cctcaaccat tatggttaat tctaggtcct
     5041 tttgtagggg catacctggg tgaattgata catgatgcac gagataacaa aaaagcactc
     5101 cgaagcgctt ggggttcatt tatcggattt ctggcaggta ccttcattaa agtggtaacc
     5161 gttattgtaa tggcggtatt ttttattcgt gaagttacct gatccttata ataacaaatc
     5221 ctcatccatt gcctcgggag gagtatgctc aaacgaagcc ttaaatgcct ctttttgctt
     5281 ttgtcgctca atgatcaaat ctgcaattac aacaggcgct tcatcaggca ggtgtttaga
     5341 taatagaagt ttaaccttct gctcatctat gtcaatccta taaaggataa ataataattt
     5401 ttcaaaatta tgatcaatca aaaatgcgat catttcgata agtaaagtgc gcgcatcatc
     5461 ctcgtttttc acggtgagcc tgctctcaga aacttccaat tcccctagac ttacaacttc
     5521 ttgcaatgtg gtatttatcc agttattact ttctgccatg tatgttgatg attgcttttt
     5581 tctctgaatc caaaacatgc ttttccatca cacctgaccc aaataaggcg taatcgtatt
     5641 tcaccggatc tgaatgatca aacatgcgca aattttctgt taactcaacc gccgcatccc
     5701 aatcggtttg cttgcgggta agtaatccca atttgcgtgc cacacgttcc acatgcacat
     5761 cgagcggaca aatcaattgg cttggctgaa tttgttgcca acagccgaaa tccacacccg
     5821 cattgtcttt gcgaaccatc catcgcaaaa acatattgag ccttttgcat gcacttttgg
     5881 atgcaggggt aggaatatgt tttttagtgc gctgaggtgc atcccggtca gaaaagaaca
     5941 aattgtggaa atgaatgagc attttttcta catgaacttc ttcacggaaa ccttcacaca
     6001 aaaacgcctc ctccagtgaa ttatgctcag cataaaagga ccgtaggaaa tatataaacc
     6061 acagagtatc cgtatagttg aaagtgcgat gcttaaaatc ggcaaatctc tttaaatcgt
     6121 cttctttatg atgtaaaata aaatcatagg gactattatg cattaatagt agcagctctt
     6181 ccgccttatt gataattgtt ttacgctgac cccagctgag catggcagtc cagaaaccta
     6241 tgatctcccg gtcttgtaat ttggtgaatt tgtgtggaag cgaaatagga tcacccttga
     6301 taaaatcagg attgttatat tttacgacca aaccatctaa atgatcagca agcgactgct
     6361 gcgatttagt tcggcttgtt cggcgagtca aatgtttctg catctgaatg ctgtttagtt
     6421 tgcgcagcag ccggctttat ggcgaaagga tttgcacggt gtaaacgcat agctttaatg
     6481 ctgaaaatta ttccggcaat gagcgatatc aaggccccga ttaccagacc ttctttataa
     6541 ttctggaata agaaaaaatc ataaaatccg tgcaatatga ccgcccctat aaaaccataa
     6601 aacatcaatc ggcgttcatg tgagcgattg aattttgcca atcctataaa ataacccata
     6661 atcgcagcaa aagtagcatg cgcaggaatc gcagttacag ctcttatgcc tgcagttccc
     6721 catgaagcac ctagtgtatc ggcaccaaaa acataaagta aattttcaat tgtcgcaaag
     6781 cccattccta ccataatagc ataaactatt ccatcaaaag gctcattaaa tgaaggcttg
     6841 cgataagcat aatatcgaag aaataaatat ttactgaact cttcacttcc ggcaacaata
     6901 atgaaggtaa atatagcaat tgaaatcaat gattcgcctt gcgctcccgg tactacatgt
     6961 ggtaatgcct tttctaaaat aatggccggc aatacactga aacatccggt taaaaaactc
     7021 accaacaaca caccaaaagg ctcccggtca tttttgtctt ttgaataaaa gaaaaacatg
     7081 atcgcaacag cgggaccaac cgctagtgcc agtaatgcaa gtgtttgaaa atccatttat
     7141 tgtgtttgat gtttgctgaa ataaatatac tgctcccgga ctgaatgtaa agagcaataa
     7201 ttcctattaa ttcaatactt attgtaaatc aattgtattc aacctaatgc tttttgcaaa
     7261 tcggcaacaa gatcctcaac atcttcaaca ccaacactca acctgatcaa tgaatctacc
     7321 aatccatttt tcaatcgctc ctcctttgga atggaagcat gcgtcatact tgctggatgt
     7381 ccgattaagc tttcaacacc tcccaagctt tctgccaaac tgaaaagatg cgttgacgaa
     7441 agtatgcgtt tcgcctcctc aaaatcatca cctttgagcg tgaatgaaac catagcacca
     7501 aacatgcgca tttgtttttt ggctactgcg tgattagcat gatcttcaaa tccgggccaa
     7561 tatactttat ctactttagg atgtgctttc aaccaatggg caattactgc agcattttca
     7621 caactccgct gaacgcgcaa atgcaatgtt ttgattccgc gtaatactaa gaaacaatcc
     7681 tgcggaccag gaaccgctcc tgttgcattc tgaataaaat ataattgatc ggcaagcgat
     7741 tgttccttta cgattactgc accatgtatt acatcactat gtccgcccaa atatttggtt
     7801 gcggaatgaa ttactatatc agcaccatga tctagaggat tttgcaaata tggcgaagca
     7861 aatgtattat caacgcaaac aagtatatta tgtttttttg caattgcaca aatggcagca
     7921 atatctacaa tgcgcaacat aggatttgtt ggcgtttcta cccatatcaa tttcgtgctt
     7981 gatttaatca gaggttcgag ttgcgaagca tcactgagat cggcaaaagt aaattccatg
     8041 ccaaagcgtg catatacttt tgtaaaagct cggtatgtgc cgccatacaa atcattgttg
     8101 gcaataatat gatcaccttc gctaaacatt ttaattattc catcagtggc agctaatcca
     8161 ctggaaaaac aaatgccgaa attgccattt tccaacgcgg caagattttg ttgcaacaca
     8221 tgacgtgttg gattctgtgt ccgcgcatat tcatacccct tatgatctcc cggcgcattt
     8281 tgaacatacg tagaagtttg gaaaataggc gtcatgatcg cccccgtact cggatccggc
     8341 tccacaccgg catgaataac ctttgtatta aatttcatat taattagcaa atttgagaat
     8401 tagcaagtta gcaagtttga gagttagcaa gtttgagaat tagctagttt gagggttagt
     8461 tggtttgaaa gtttattttg aaactttcga tttttcagtc tgaatttcat ttatttgctt
     8521 ttgaaatcag ctagtttgct aattctcaaa atgtaattaa ttgatacttg gtgtgcacaa
     8581 agatagccat tagagaattt gattacaaat tagctttagt agtggaaatg atgctattta
     8641 gtacccgttt gatggcataa agtttatcaa gataatcctt catatcaggg tattcctctg
     8701 attggttgcc gagtaacagc caatattcgg tttcttcagc ctctttcgct gcaattttta
     8761 ttttgtgcag aaaatctttt ttgctttcag cattttgggc ttcaatgaca tttgcaccaa
     8821 tacctgtgcc tgatcgaaat aattgtttgc tataagagta tttatacccc tgatccagtt
     8881 gagagcatag gtgcattatg tcaagtgaaa attcgaacgt taatttgaca gcgatacttt
     8941 tattagcaga atgtggaagg ttcatttgtt tttaaaagta aacctatttc aatattctct
     9001 gctcaaattt ttcaaaatcc accgtaagcg cttacgtctc taaaatttgt aaagattgga
     9061 tgttcataaa aaatttcagt gacatccccc ccctcgcact actcttaaat tcccatactc
     9121 ctacactcct acatcacatt cccacacacc cacacaccca cactctcaca cacccacacc
     9181 cacactctca atcaccgaca ctctcacact ccctcactcc cacgcacctt caaacttgct
     9241 aacttgctaa ctctcaaact tgcaaactag aactagagtt ccaaggtaag tatatcccaa
     9301 ataggctaat taaaacgtgt gcacaaccag cccgcttctc aacttcggtt caaaccaggt
     9361 tgatttcggt ggcataacgt ttccactatc cgcaatattg attaattgct gaatacttac
     9421 agggtataac gaaatagcaa gcgccatttc gccactgttc acacgttttt ctaattcacc
     9481 caaaccgcga atgcccccaa caaaatcaat ccgcttgtct gtgcgctgat cttttattcc
     9541 aagaattgga tcgagaagat gattagataa aatggttaca tctaaaacat caattgggtc
     9601 attgtcatta tatgtgccct gtttcgctgt gagtttatac cagtgattat tgatgtataa
     9661 tccgaaatga tgtaatcctt caggtttata tatgccttca tgtttttcaa tagaaaactt
     9721 ttcacctatg cgatcaagta attgatcttc acttaatcca ttgctgtctt ttacaacgcg
     9781 attataatcc atgatcataa gctgactgct tggaaaaatc acactcaaaa aataattgta
     9841 ttcttcttct ccgttgtgat ttggatttgc atccttcatg cgttgaccaa cttttgcact
     9901 ggatgcactt ctatggtgac cgtcggcaat gtatgtaaat ggaactttcg aagaaaataa
     9961 ttgtgtcaac cccgctacat cttgtgcatc actaattacc caaaaagtat gttgaatgcc
    10021 gtcatcagca gtaaaatcat agatcggtgt acctgccaca actttatgca tgatcgcatc
    10081 gatatctgca acatcaggat aagtcagaaa tacgggctca gggtgacatt gtaaatcata
    10141 catgtgacga atacggtcat tttctttttc cggacgggtg aattcatgct tcttaataat
    10201 gtcgtcaaag taatcagaga tagcagaatt ggcaaccaat cctacctgcg catgaccatt
    10261 cataatctgg cgataggcat acatgttgcg ggtggcatcc tgaacaaaag tgccattatt
    10321 ttgaaatcgc ttccagtttt ctacagcctt tgcataaaca gcatcgtcat aatgatctac
    10381 ctcttccggc aaatcaatct ccggcttgca aacatgcaag aaactcaaat catttcctgc
    10441 agcctccacg cgggcttctt cactgttcaa tacatcgtag gggcgactag ccacctgttc
    10501 ggcttgagct gcttgtgggc gccagccttg gaagggtctt atgattgcca tgtgcaaatt
    10561 ttgccgcaaa gatagggtat atggcaatta ctattcccct tcccaattcg gctcccaatt
    10621 tgcacactta aaaaaaaact atatttttaa ggtataataa taacgtatga caaagaacta
    10681 caaattagtt acagctcaaa aattggcagc gtattctgca atggctgccg caataacagc
    10741 atcaatgagc gatgcaaatg ctgaggtaat ctatacagat atcgatgacg taactatcgg
    10801 tgtcggcgat atttaccaac tggatatcga tgcagatggc accgctgatt tcatgtttgg
    10861 tggcggctct acaatgagct ccggaggctc cacatggtcg tttgtgtctg catttggcta
    10921 tgttaccagc tacactgttg gcaatgccga caatcaaatt atcggctaca ccggaagctt
    10981 ctataactat ggaagtgccc tcgaagatgg tgctctcatc ggccctgatg gaccatggct
    11041 gaattaccca agcctttcta atagtgctgt gctggcatcc aatttctatg gctcaacata
    11101 cggacagttc ccgggtctcg gagagcgatt catgggtatc aaattttcat cgaaaggaaa
    11161 cctgcactac ggttggattc gtgtagtagc tgatatcgat cccgtttatg caaccattat
    11221 ggattatgca tatgaaacag taaacgatgt gccaattgaa gccggttcta ccattagcct
    11281 tgttggggtg gaagaattaa gtgcaggcac ggtaaatgtg tatgctttta attcatcagt
    11341 aagggtagtt gtcaaccagg atcttactag tcctcagatt cgcataaccg acctccacgg
    11401 tgctaccgta ttcactgcac cacttaacaa tcgtcaggct accatcgacc tgaaaaattt
    11461 ctctaccgga aattatgtgg tttctgtgct gtcggcagaa ggaacttcaa acaaacaagt
    11521 atttatcaat tgataaaata aaacaataaa aaagcggagc ctgataatca gtgctccgct
    11581 ttttattttg ttggcaatag gaagtaaccc tcattaattt actctgaagg tgtcaattcc
    11641 tttatccaga aaatcaagaa tttgtttagc agctgcaata cctgcattta catttgcttc
    11701 ctcagtttgc gcacccatct tttttgcagg atagaaaatc cgtttcgcaa atttttcttt
    11761 gaaaacagat tcctgtttag gaataaaatc acttacgtat tgaatatcgc tgcgctcttc
    11821 catcaacttt aacagatcat cttcatttac aacatcaaca cgagcagtgt tcactaacat
    11881 ggcaccttta ggcatcaatc gcaacaggtg ataattaatt acaccctttg tctccgcagt
    11941 tgacgggaga tgtaaactga tgtactggca atgttcaaac acatcttcaa agctgctcgc
    12001 aggtgttaca ccgtcaattt ccatcgccaa tcgatccatg ttgcgtttgt aggcataaac
    12061 atccatgcca aatccgcgtc caatctgcgc catgtagcga ccaatatttc cataacccac
    12121 aatacccagg ttttttcctc gtaattcagt acccgagcta ccgctaaatt gtttgcgcgc
    12181 cataaataac atcatcccaa atgcgcattc agcaacagca tttgcatttt gtcccggcgt
    12241 attcatgcaa acaacattat ttgccgatgc agatttcaga tcaagattat catatccggc
    12301 ccccgcgcga acaacaattt tcagatgatt gcctgcattt atgacatcgg catcaacaat
    12361 atcgctgcgc acaatcaacg catcagcatc tgcaaccgca tttagtaatt gttgcttttc
    12421 agtatagttc tccaataaag caagttgatg accgccacct gttaccaccg ttcttatttt
    12481 ctcaacagct tctgcggcaa atggcttttc agtagccaat aaaatagtac ccatgtatgt
    12541 ttatttaatt caatgattta ctttcagaaa ttgagttatg tcacctggca actttgatga
    12601 agaagtttag ttttagcaat aaagcatcaa ccttttttcg ctgcaaattc attcatcagg
    12661 tttaccagat gctgaacaga atcaagacca agagcattat ataatgaagc acgcatacca
    12721 cctaccgagc ggtgacctgc caacccaacg caattatttg ctttagcaaa agctaggaat
    12781 tcaggctcaa ggtcgcgatt ttccataata aagttcacgt tcatccatga gcggtcttct
    12841 tttgctaccg tacccttaaa taatgggttg ctgtcgattg cattatataa taactcagct
    12901 ttcgagcggt taacagattc aataatattt agtccctgct cttttatcca tcgcaaagtc
    12961 agcatggtaa cataaataga aaaaaccggt ggtgtattaa ataacgagtt ctccttaata
    13021 tgcgtgcgat aatccatcat agttggaatc gtgcgattca ccttaccaag aagactttct
    13081 ttcaaaataa ccacggttac acctgaagaa ccgatatttt tttgagcgcc ggcgtaaatg
    13141 aggtcaaatt ggctgaaatc acaatttcta ctgaagatat cagagctcat atcaattata
    13201 tatggcaccg ggctctttgg atatgcgtgg tattgcgttc catatatggt attatttgag
    13261 gtaatatgga aatatttaag attgtccgga atctgataac cctttggaat aaaagtgaaa
    13321 ttatctggtt cagaagtagc cagttccaac acatttccaa actgtttagc ttctttctga
    13381 gcctttttcg cccatgtacc tgtattacaa tagcctgcag tttcagatgc atccagcaag
    13441 ttcataggca ccataccgaa ttgagtagaa gcaccacctt gaaggaatag tactttataa
    13501 gagtcgtcca aaccggcaat ttccttcacc aaagcagtag cttcatccac tacggcttga
    13561 aaatctttag atcgatgaga aatttcgagg agcgacattc cgaggccgtt aaactcagtc
    13621 acagcttgag ctgcctggct tattaccggt gccgggagta tggccgggcc ggcattaaaa
    13681 ttgtggactt tcatattttt tttatggttt acttgttttt gttgtttttt ccgctacctt
    13741 tgcactccca tttcgttctt tatacaggga aacagagttc aaaacgcggc tcaaaaatac
    13801 gatttccgcc cttttaaaaa agtgatgaaa gtcagtgaag ggatttaggc tgaaacgtag
    13861 gaactgcggg ggtttttaaa aatctttaat tttttttgcc caaaaatttg acaatgcaat
    13921 aaaggttgca ttacctttgt gtcccatttc gaaaaaaatg tggccccgaa agttggggta
    13981 tacatataag ttctttgaag tattggagaa acaacaagta gcaagttcag gtaaaaatta
    14041 ataattactg aagtcaattt aatacttaca ttttttaaca atggagagtt tgatactggc
    14101 tcaggatgaa cgctagcggc aggcttaata catgcaagtc gaggggtaac aggtccttcg
    14161 ggatgctgac gaccggcaaa cgggtgagta acacgtatgc aatctacctc taactggggc
    14221 acaactttgg gaaactgaag ctaattcccc gtaacaatat gaaatcgcat ggttttgtat
    14281 ttataattcc gatggttaga gatgagcgtg cgtccgatta gatagttggc ggggtaacgg
    14341 cccaccaagt caacgatcgg tagctgatgt gagagcatga tcagccacac gggcactgag
    14401 acacgggccc gactcctacg ggaggcagca gtagggaata ttgggcaatg gaggaaactc
    14461 tgacccagcc atgccgcgtg aaggatgaat gtcctatgga ttgtaaactt cttttgttag
    14521 ggacgaaacc cctggattca tccggggctg acggtaccta aagaataagc accggctaac
    14581 tccgtgccag cagccgcggt aatacggagg gtgcaagcgt tatccggatt cactgggttt
    14641 aaagggtgcg taggtggatt agtaagtcag tggtgaaagc cccgggctca acttgggaat
    14701 tgccattgat actgctagtc ttgagttcgg ttgaggtagg cggaataagt catgtagcgg
    14761 tgaaatgctt agatatgact tagaacaccg attgcgaagg cagcttacca agccgacact
    14821 gacactgagg cacgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc
    14881 ctaaacgatg atcattcgac attagcgata tactgttagt gtccaagcga aagcgttaaa
    14941 tgatccacct ggggagtacg cccgcaaggg tgaaactcaa aggaattgac gggggtccg
//
LOCUS       DAOQOU010000065        14973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2027_length_14973_cov_6.719064, whole genome shotgun sequence.
ACCESSION   DAOQOU010000065 DAOQOU010000000
VERSION     DAOQOU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2027_length_14973_cov_6.719064"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /gene="uppS"
                     /locus_tag="PLM90_07305"
     CDS             <1..561
                     /gene="uppS"
                     /locus_tag="PLM90_07305"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HMZ89215.1"
                     /translation="YAFSTENWKRPEEEVNALMELLVRTIRRETPELDKNNVRIGMIG
                     DGHSLPPACIAELEEAKKMTADNTGLNLILALSYSGRWEITHAVQQIAKDLVNGKIKP
                     EEITEQTIADKLNTAAMPEPELLIRTSGEYRLSNFLLWQTAYTEYYFTETLWPDFDKE
                     ALFNAIIDYQNRERRFGKTTEQIQVK"
     gene            558..3242
                     /locus_tag="PLM90_07310"
     CDS             558..3242
                     /locus_tag="PLM90_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMZ89216.1"
                     /translation="MKRTLLSLLLCGVMIATLSVTGWSQTPGLDSNSVDYGNPKKYTI
                     AATEIRGVTSPNLDLNSLIVRTGLTPGLPLTIPGEDFSVAIKSLWKMRLFSDVQIFVE
                     KTLGDQVWLTIQVTELPKVSKYAFTGTTKSEDEDLRPQLDIIKNVTPFADYTKTHIYN
                     TVREYYAEKGFLNAEIDVYGKPDTSFRNSVIVYIDVVKNAKVKIATIDIHGNTEMSDR
                     EVLKNMKETRVRTQIKPFYNDPENPISDDVLSVKGVVNVLSSLSVSNIMDFVYDRAQI
                     RIFNQSKYDEAKARTDAQAVITYYKSKGYRDAQMKLDSVYNVSDRSIGLSYTIDEGTK
                     YYFRNITWKGNTKFTDKQLDSILDISKGQVYNQSYLDQRISFDLTKPDITSKYMDDGY
                     LFFRINPVEIWAQDDSIDLEIRISEGPQAIIDKIIISGNTKTNEHVIRRELRTLPGQK
                     FNRSLVIRSQREIIALGLFDAEQIGINPIPHPESGTVDIEYTVVEKPSDQLELSAGYG
                     GQGYGVLASVGIVLNNWSTTQMFKKEGWKPVPMGDGQKLSFRVNTTGRLYQAYNIGFV
                     EPWFRGKKPNSLSFSLTRTQLGYNIPGSTYDSLEGKFIANGASLGYGIRLKWPDDYFT
                     LVSTVSFYNYSLTDYPYFIATNGDYNNFSIKETLGRNSLDDQQFPKSGSSISLSLQFT
                     PPYSALNSKDYGELGTEEKYKFIEYYKWRFNADWYTPVVGKFVFRASMKFGFLGYYNS
                     DVGIPPFERFQLGGDGLSGGYTNTFVGTDIISMRGYEVFPQTNSSSARTEAIFNKYTL
                     ELRYPIVKSQSANIYALAFAEGGNLYADIDNYNPFEIKRAAGLGVRAWLPMFGLLGVD
                     YGIRFDDLVPGDLEQASGFFDYITRNGKFTVVLGFEPE"
     gene            3258..3785
                     /locus_tag="PLM90_07315"
     CDS             3258..3785
                     /locus_tag="PLM90_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ89217.1"
                     /translation="MKKQLILATIVMAFAVTAQAQRFAYVNTDYILDNMPEYKKAQEQ
                     IDKLTQEWRGEADKKQKEIDELYRSFQNEQYLLTEDQKKAKISEIETKEKALKDYQKS
                     VFGYEGLLYQKRQELVKPIQDKVYEAIEKYARERGYDFIFDKSSSTTILWANPDNDKS
                     DEIIKKLGYTPTKTN"
     gene            3821..4321
                     /locus_tag="PLM90_07320"
     CDS             3821..4321
                     /locus_tag="PLM90_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ89218.1"
                     /translation="MKNKLMLLFAIAVMAISQADAQKIGYLNTNELLVQMPEVKKADS
                     VLNQYAEEMQKIYAAYVMEYQQKLADYQNNAASWSEVKRESVEKDLGNLQIRISDFEK
                     ESNDKLEAKKEELYGPILDNVKAKIKLVGDENKFTTILDGSAMLYVGIDAVDILPLVK
                     KKLGLQ"
     gene            4422..5504
                     /locus_tag="PLM90_07325"
     CDS             4422..5504
                     /locus_tag="PLM90_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89219.1"
                     /translation="MKRILTLVLVLTFSVHAYSQSKKDQALEKALTAIAMMDSGYITE
                     SIDLLNEAIKLDPDRYDYPYEVAYAYYLMKDYNKSIEVLEKLSKHKDVTDQLYQLLGN
                     SYDIIGDPDKAFDAYDRGLKKFPKSGILFLEKGNVYWGQKKYAEALPFYEDGIAADPM
                     FSSNYYRAARIYCATDNPFFGIMYGEIFLNLEPSTERSQEISALLFDTYKSNIHVTSD
                     TSASIEFCSNNLVLDKESMKRMSEGILPFCMTAETTMLIYVGFSKEITLESVCKLRSS
                     FIDAWYQMGNDKKYNIELFKFQKDLADKGYYDAYNHWLFMNGDGAAFDEWYKTHQDEF
                     TNFANYANQHRLQLNDKTKFLVADFR"
     gene            5550..6365
                     /gene="murI"
                     /locus_tag="PLM90_07330"
     CDS             5550..6365
                     /gene="murI"
                     /locus_tag="PLM90_07330"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMZ89220.1"
                     /translation="MFPIGVFDSGYGGLTVLRSFKKALPEYDFIYLGDNARTPYGNRS
                     FETVYEYTLECVNHLFNMGCRLVILACNTASAKALRTIQQNDLPKIDSSRRVLGVIRP
                     TAEIIGKMTHTGTVGVFATAGTVQSESYIIEIAKAWPKITVVQEACPMWVPLIENNEY
                     NKPGADYFVQQHMLQLLQKAPELDTILLGCTHYPLLIEKIKQFAPQGTTIIAQGDIVA
                     ESLVDYLKRHPEMESQCSKNGTMQFYTTDDTANFNAHASAFFGSEVQSTFLHL"
     gene            complement(6354..7454)
                     /locus_tag="PLM90_07335"
     CDS             complement(6354..7454)
                     /locus_tag="PLM90_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HMZ89221.1"
                     /translation="MSAPKPLNILIAPLDWGLGHATRSIPIIKACVDRGHTVVIASTG
                     RSLALLRREFPELTCLDVPAYDIAYQEKGSFILKILGQLGKIFSGIRREHRATKEIVR
                     LHQIDLVISDNRYGFYHKKIHSVIITHQMMVKIPSFVIAEPFVYGWLQLQHKRFNTIW
                     IPDVAGDANISGDLSHKYPVLKRTHYIGILTRFSRPLSLPAAEKTILAIISGPEPQRT
                     LFERLIVTQGRLLPHQFILIQGISDEHRDEQLTDNIRVISHLPGKELYDLILRCDVII
                     SRGGYSTLMDLALLNKKCIFVPTPGQTEQEYLVKELGEKGLVVAGDQSTFRLEDALEK
                     VQHTKGFYIPSNEQTFIPFLEAAIAEAVKSKV"
     gene            7527..7700
                     /locus_tag="PLM90_07340"
     CDS             7527..7700
                     /locus_tag="PLM90_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89222.1"
                     /translation="MKNGKKLLWLGAITIMLITTSATCEGGGKKCDCPKFSQIGPNNN
                     DAVASQAAANTLD"
     gene            7771..8280
                     /locus_tag="PLM90_07345"
     CDS             7771..8280
                     /locus_tag="PLM90_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HMZ89223.1"
                     /translation="MLTKKVEAALNKQVEMEGYASNYYLSAASWCDSKGLQGAAGFLY
                     GHADEERMHMMKLFHYINDAGGHAEAPNVKQPPLKFKNLKELFTLVFKHEVEVTKAIN
                     NLVDLCMGEKDHSSYNFLQWYVAEQHEEEKLFRTVLEKIELLGPDDTRGLYWIDRELA
                     ALTAAPSAE"
     gene            8360..9418
                     /locus_tag="PLM90_07350"
     CDS             8360..9418
                     /locus_tag="PLM90_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89224.1"
                     /translation="MNTTTVNYLNIALMLLSAIVAFIIPFELFLFSYAVLGPLHYLTE
                     IGWLHKRKYFSPKKNDYIPLVIITLLIAIPAIISYMFVHNGERTSTGQLRLTEGQHNF
                     IAFFQDQNTATALMFLAFSISLIFILIRNNTHRTIAVGLLAIIGFLIRSNHFVDVLFA
                     MFLPTLIHVFLFTGAFILVGALKSKSTSGYLSILVFLGCSLSFFLIMPDGGGYHISDY
                     AKGSYDVSFFGLNHQLFSSFLHESNPTNDMIYNSSTGILITRFIAYAYTYHYLNWFSK
                     TSVIKWHDVPKGWLVTIGVLWVSAVTLYYINYETGLMCLYFLSFLHVVLEFPLNFQSF
                     KQIGEQVRLRLSGKSAAT"
     gene            complement(9520..10056)
                     /locus_tag="PLM90_07355"
     CDS             complement(9520..10056)
                     /locus_tag="PLM90_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89225.1"
                     /translation="MIGTILLVIAGIFALLIIAGLFVPRSYKFSKSIVISGDKKHIFN
                     TIDDLQTWSKWSQWSPDRDPAITITCSNPSAGVNSFMEWKGKKMGAGKMEIVATEPYK
                     NITVATAFNKGLFKMDFDFSLEEIKDQQYSVKWTVRGKTKRGGFAKILGRMLPKMMGK
                     DMEIALKLLKHYCEGTLS"
     gene            complement(10097..10879)
                     /locus_tag="PLM90_07360"
     CDS             complement(10097..10879)
                     /locus_tag="PLM90_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89226.1"
                     /translation="MHIRKFFATMLFVASGMMLHAQTTNTIPNNLDKPDIIKFNLSGA
                     VFGNYTLQYEHVLNVKQSVGMSISFAPNMDLPFKNALVNIFEGNDQAITAINSTQLNT
                     FSITPEYRFYSEVKGAPFGVYGASFIRYTHMTHRQIYTFNLSDGEHHPDVETTFNGVG
                     VGEMVGVQWKIGNSMTLDWWILGPFIGYQTGKSHGDDDRPITPEDKIKLENDIESVPI
                     PGWNIDATVTDNGTTGLGVVDADLSGLFLGARVFGICLGYRF"
     gene            complement(10987..13815)
                     /gene="uvrA"
                     /locus_tag="PLM90_07365"
     CDS             complement(10987..13815)
                     /gene="uvrA"
                     /locus_tag="PLM90_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMZ89227.1"
                     /translation="MKTKKSYPNGHIDAIFIKGAREHNLKNIDVAIPRNRLVVVTGVS
                     GSGKSSLTFDTLYAEGQRRYVESLSAYARQFLNRLDKPDVDYIKGIAPAIALDQKVTT
                     RTTRSTVGTLTEIYDYMRLLYARVGRTYSPISGREVKKDEVNDVVDYICSLPENSRIY
                     LLVKLHVDKDRPLNDQARVLQQKGFTRFYANGSIVKIEDILEDPKLIAPKTPVHLLID
                     RVVLAKERAEGSNVPEVNEETRQRIADSVDIAFSEGHGACHVVAEGIEERSFSNNFEA
                     DGMQFEVPSPHFFNQNNPYGACKRCEGFGNVIGIDHDLVIPDKNLSVYQGAVACWKGE
                     KMRTWLDDLLKAAKKFDFPIHKPISQLTKAQYQLLWTGNEHFWGLDRFFKMLEENAYK
                     IQYRVMLARYRGKTTCPECNGSRIRPDADYVLINNMSITKLVDMQVEDLAVFINDITL
                     NAYETKVASRILLEIRNRIVYMLDLGLGYLTLNRRSNTLSGGETQRINLTRTLGSNLT
                     SSLYILDEPSVGLHPRDTERLVTVLKRLRNLGNTVVVVEHEEDVIRNADHIIDIGPAA
                     GIHGGKVVSEGNLEEILADKESLTGNYLSSTMQIPLPRTRRKSFKKLRIRQASMHNLK
                     QIDVTIPLQNLVAVSGVSGSGKTTLIKHILYPELMKMLDDASDTAFISRVIEGDVSAI
                     TQVELVDQDPIGKSSRSNPVTYVKAYDEIRDLYARQPLAKMRGYKSGFFSFNVDGGRC
                     EMCKGEGEIIVEMQFLADVHLVCEDCNGKRFKEEILEVQYKNKNISELLGLSVEEAIH
                     FLQDEKDIVAKLQPLADVGLGYVKLGQSSSTLSGGEAQRVKLASFLGKGKSKEHILFI
                     FDEPTTGLHFHDINKLMDAFNALIECGHSVLVIEHNLEVIKCADYMIDLGPEGGDKGG
                     YLLYQGAPEGIVTVKESYTGQHLKNKLK"
     gene            13988..14566
                     /locus_tag="PLM90_07370"
     CDS             13988..14566
                     /locus_tag="PLM90_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ89228.1"
                     /translation="MQSRTLSDDKLVELYLTGEESALGELLNRHKDRIYTYIYLFVRD
                     THTAEDLLQETFIKVIDKLRSGKYQEQNKFLPWISRVAYNLCIDYYRREKRMPKVVTR
                     DGFDIFSVLKFSEPGAEGKVMESEFSSRVRKLLEYLPEEQREVVVLRHYMDMNFREIA
                     EMTGVNINTALGRMRYALINLRKLMETKNISI"
     gene            14634..14873
                     /locus_tag="PLM90_07375"
     CDS             14634..14873
                     /locus_tag="PLM90_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89229.1"
                     /translation="MIKTITQDNLIRFIYRETTAEEASLIEEALRSDFDLKELYEQML
                     ESKAELDKVRLQPSQKVIDNIMNYSKNSAHMEHLL"
BASE COUNT         4444 a         3142 c         3051 g         4336 t
ORIGIN      
        1 tatgctttca gcaccgaaaa ctggaaacgc cctgaggaag aggtgaatgc actgatggaa
       61 ctgctcgtaa gaaccatacg ccgcgaaacc cccgaactcg ataaaaataa tgtgcgcatc
      121 ggcatgattg gcgatggaca ttctttacct cctgcttgca ttgccgagct cgaagaagca
      181 aaaaaaatga ctgccgataa cacaggcctg aatcttatac tggctctgag ttattccgga
      241 agatgggaaa ttacccatgc cgttcaacag atcgctaaag atcttgtaaa cggtaaaata
      301 aaacccgaag aaattacaga acaaaccatt gcagataaac tcaatactgc tgccatgcct
      361 gagcccgaat tgctcatacg cacaagtggt gaataccgat tgagtaactt tttgctatgg
      421 caaaccgcct atacagagta ctactttacc gaaacactgt ggcccgattt tgataaagag
      481 gcactcttta atgctattat cgattatcaa aaccgcgaac gcaggttcgg aaaaactacg
      541 gaacaaattc aagtgaaatg aaaagaacgc tgcttagcct gttattgtgt ggagtgatga
      601 tcgctacatt gtccgttacc ggatggtcgc aaacaccggg actcgactct aatagtgtgg
      661 attatggtaa tcctaaaaag tataccatcg ccgcaaccga aattcgcggg gttacttcac
      721 ccaatctcga tcttaatagt ttaatcgtac gcacaggtct tactcccgga ttgccactta
      781 ctattcccgg tgaagatttt agtgttgcca ttaaaagcct ttggaaaatg cgcctgtttt
      841 ccgatgtgca gatatttgta gaaaaaactc ttggcgatca ggtatggctg acgattcagg
      901 taactgaatt gccaaaagtg agtaaatatg cattcaccgg caccactaaa tcggaagatg
      961 aagaccttag accgcaattg gatatcatta aaaatgtgac accatttgcc gattacacca
     1021 aaacgcatat ttacaatacc gttcgggaat attatgcgga aaaaggattt ttaaatgcag
     1081 agatcgatgt ttacggaaaa ccggatacat cttttagaaa tagcgtgatc gtgtatatcg
     1141 atgtagtgaa aaatgcgaaa gtgaaaatcg caactattga tattcatggt aataccgaaa
     1201 tgagcgatcg cgaagtgttg aagaacatga aggaaacacg cgtgcgcacg cagatcaagc
     1261 cattctataa cgatcctgaa aatccaattt ccgatgatgt attatcagtt aagggtgtag
     1321 tgaatgtgct gtcgtcgctg agcgttagca atatcatgga ttttgtttac gaccgcgcac
     1381 aaattcgcat tttcaatcag tcgaaatatg atgaagcaaa agcgcgtacc gatgcacaag
     1441 ctgtaatcac ttactataaa tcaaaaggct atcgcgatgc tcaaatgaaa ctggattcag
     1501 tgtataatgt aagcgataga tcaattggat tgtcttatac catagatgaa ggaactaaat
     1561 attatttccg caatatcaca tggaagggaa atacgaaatt taccgataaa cagctcgatt
     1621 ctattctaga tatcagcaaa ggacaagtat ataatcagag ttatcttgat caacgcattt
     1681 ccttcgacct taccaagccg gatatcacgt ctaaatatat ggatgatggc tatttatttt
     1741 tccgtatcaa tcctgtggaa atttgggcac aggacgatag tattgacctt gaaattcgta
     1801 tatcagaagg acctcaggct atcatcgaca aaattattat cagcggaaat acaaaaacca
     1861 atgaacatgt gattcgtcgt gagttgcgta ctttacccgg acagaaattt aatcgttcat
     1921 tagttattcg ttcacaacgt gagatcattg cacttggatt atttgatgcg gaacaaattg
     1981 gtatcaaccc gattcctcac cctgaatcag gaacagtaga tattgaatat accgttgtag
     2041 aaaaaccatc tgatcaatta gaattgtcgg caggttatgg cggacaggga tatggtgttc
     2101 tcgcatcagt tggtattgta ttaaacaact ggagtaccac acaaatgttt aaaaaggaag
     2161 gatggaagcc ggtgccaatg ggcgatggtc agaaattgtc tttccgtgta aatacaacag
     2221 gtcgtttata ccaagcatat aatatcggat ttgttgaacc ttggttcaga ggtaaaaaac
     2281 caaactcttt gtcattcagt ttaacacgta cgcagcttgg atataatatt cccggttcta
     2341 cttacgattc actcgaaggg aaattcatcg caaatggtgc atctctcgga tatggtattc
     2401 gcttgaaatg gcctgatgat tattttacgt tagtaagtac tgtgagcttc tacaattaca
     2461 gccttactga ctatccttat ttcattgcca cgaatggcga ttacaataac ttcagcatta
     2521 aagaaacgct tggtcgcaac tcattagacg atcagcagtt tccaaaatca ggaagcagca
     2581 tttcactctc gttgcaattt acaccaccgt attccgcttt aaattcaaaa gattatggtg
     2641 aattgggaac ggaggagaaa tataaattca ttgaatatta taaatggcgc tttaatgccg
     2701 attggtatac acctgttgta ggcaaatttg tcttcagagc atctatgaaa tttggattcc
     2761 taggatacta taatagcgat gtgggtattc cgccattcga aagattccaa ttgggtggtg
     2821 atggattatc aggaggttat accaacacat ttgtcggcac agacattatt tccatgcgtg
     2881 gttacgaagt gttcccgcaa accaacagca gttctgcgcg cactgaggct atcttcaaca
     2941 agtataccct tgaattgcgc tatcctatcg taaaatcgca atctgccaat atctatgctc
     3001 tggcttttgc cgaaggtgga aacctttatg ccgatatcga taattataat cctttcgaga
     3061 tcaaacgcgc ggccggactg ggtgtcagag cttggttgcc gatgtttgga ctcctcggtg
     3121 tagattatgg cataaggttt gacgatctgg tacccggaga ccttgaacaa gccagtggat
     3181 tcttcgatta catcacccgt aatggtaaat tcacagttgt tctcggattt gaaccggaat
     3241 aaaaaaaacg aaagaacatg aaaaagcaac ttatcctcgc taccatcgta atggcgtttg
     3301 ccgttaccgc tcaggcacag cgctttgcgt atgtgaatac tgactacatt cttgataata
     3361 tgcctgaata taaaaaggct caggagcaaa tcgataaact gacacaggaa tggcgcggcg
     3421 aggctgacaa aaagcaaaaa gagatcgatg aattgtatcg ctccttccaa aatgagcaat
     3481 acctgttaac ggaagatcag aagaaagcaa aaatttctga gattgaaacc aaagaaaaag
     3541 cactgaaaga ttaccagaaa tcggttttcg gctacgaagg cttgctttat caaaaacgtc
     3601 aggaactggt taagccaatt caggataaag tgtacgaagc cattgaaaaa tatgcccgtg
     3661 aacgcggcta tgatttcatt ttcgataaat ccagcagcac taccatactt tgggcaaatc
     3721 ccgataatga taagagcgat gagattatca agaaattggg atacacacca acaaaaacga
     3781 attaacacat ttataatcca acaatcagta caaccatatt atgaagaaca aattaatgct
     3841 cttattcgcc attgcagtta tggcgatttc acaagccgac gcacaaaaaa tcggatacct
     3901 caataccaat gaacttctgg tgcaaatgcc ggaagtgaaa aaggctgact cagtgctcaa
     3961 tcagtatgcc gaagaaatgc agaaaatcta tgctgcttat gtaatggaat atcagcagaa
     4021 attggccgac tatcagaata acgcagcttc atggagcgaa gttaaacgcg aatcagtgga
     4081 aaaagattta ggtaatcttc aaatacgcat cagcgatttt gagaaggaat caaatgataa
     4141 actggaagcg aagaaagaag aactctatgg tcctatcctt gacaatgtga aagcgaaaat
     4201 caaattagta ggtgatgaaa ataaattcac caccattctc gatggctcgg caatgttata
     4261 tgtgggcatc gacgcagtgg atattctgcc attagtgaaa aagaaactag gattgcaata
     4321 atcgcatcca taaattattg aaaacagggc tgtgtctgat gatgcagccc tgtttgtttt
     4381 ataggcattg atgccgtaaa tttgaaatca aacaaccagc catgaaaagg atacttacgc
     4441 tcgtattggt attaaccttc agtgttcatg cctattccca atcaaaaaaa gatcaggcgc
     4501 tggaaaaagc attgaccgct attgccatga tggactccgg gtatattacc gagagtatcg
     4561 atttgctgaa cgaggctatc aaactcgacc ctgatcgata tgattatcct tatgaagtag
     4621 cctatgctta ttacctgatg aaggattata ataagtctat agaagtactt gaaaaattat
     4681 ctaaacacaa agatgttacc gatcaattat atcagctgtt gggcaatagc tatgatatca
     4741 tcggcgatcc tgataaagca tttgacgcat atgacagagg attgaaaaaa tttcccaaat
     4801 ccggcatact ttttcttgaa aaagggaatg tctattgggg acaaaagaaa tatgccgaag
     4861 cactgccatt ctatgaagac ggtattgctg cagacccgat gttttcatct aactattatc
     4921 gcgcagccag aatttattgt gctaccgaca atccgttttt tggcattatg tatggagaaa
     4981 tattcttgaa ccttgagccg agtacagaaa gatcgcagga aatcagcgcg ttgttattcg
     5041 atacctataa gtctaatatt catgttacaa gtgatacttc cgcatcgatt gaattttgta
     5101 gtaataatct ggtattggat aaagaatcaa tgaaacgcat gagtgaagga atactgccat
     5161 tctgtatgac tgcagaaact accatgctca tatatgtcgg attttccaag gagataacac
     5221 ttgagtctgt ttgcaaattg cgcagttcat ttattgatgc atggtatcaa atggggaatg
     5281 ataaaaaata taatatcgag ctgttcaaat tccaaaagga tctggctgat aagggatatt
     5341 atgatgctta caatcactgg ttgtttatga atggcgatgg agcggcattc gacgagtggt
     5401 ataaaacaca tcaagatgaa ttcaccaact tcgcgaatta cgctaatcaa cataggctgc
     5461 aattaaatga taagactaaa ttcctggttg cagattttcg atgaacaggt gattgctatt
     5521 gcaatatcaa tttccactac ctttgctgca tgtttccaat aggcgttttc gattccggtt
     5581 atggtggact gacggtattg cgctcattta aaaaggcatt gccggaatac gatttcatct
     5641 acctcggcga taatgcccgc acaccctatg gaaacagaag tttcgaaacg gtatatgaat
     5701 atacactcga atgtgtcaac cacctgttta acatgggctg ccgcctcgtg attttggcat
     5761 gtaatacagc ttcagcaaaa gcattgcgca cgattcagca aaacgattta ccaaaaattg
     5821 attcctccag aagagtgttg ggcgtcatca ggcctactgc cgaaataata ggcaaaatga
     5881 cacataccgg tactgttggt gttttcgcta ccgccggaac tgtgcaatca gaaagttata
     5941 tcattgagat agctaaagcc tggcctaaaa ttactgttgt gcaagaagcc tgccccatgt
     6001 gggtaccttt gattgaaaat aatgagtata ataaaccggg tgccgattat tttgtgcaac
     6061 aacatatgct gcaattattg caaaaggctc ctgagctgga taccatctta ttgggatgca
     6121 cccattatcc tttattaatt gaaaaaataa agcaattcgc accacaagga actactatca
     6181 tcgcacaagg tgatattgtt gctgaaagtc tggttgatta tttaaaacgt catccggaaa
     6241 tggaaagtca gtgcagtaaa aatggcacta tgcaatttta taccaccgac gatacggcaa
     6301 attttaatgc acatgcttct gcatttttcg gcagtgaagt gcagtcgact tttttacacc
     6361 tttgatttta ctgcttctgc aatcgcggct tccagaaatg gaataaatgt ctgttcattg
     6421 gaaggaatat aaaatccctt ggtatgttgt actttttcaa gtgcatcctc caaacggaat
     6481 gtcgactgat ctccggcaac aaccaatcct ttttcgccta attcttttac taaatattct
     6541 tgttccgtct gccccggagt aggaacgaaa atgcactttt tgttgagcag ggcaaggtcc
     6601 atcaatgtac tatatccgcc gcgggaaata attacatcgc aacgcaaaat gagatcatat
     6661 aattctttgc ccggtaaatg cgaaatcacc ctgatgttat ctgttaattg ctcatcgcga
     6721 tgttcgtcgg agatgccttg aatcaaaata aattgatgtg gcaataatct accttgtgta
     6781 acaattaatc gctcaaataa agtgcgctga ggttcaggcc cgctgataat ggcaagaatt
     6841 gttttttctg cggcaggtaa tgacaatggt cttgaaaacc gggttaaaat accaatgtaa
     6901 tgtgtgcgtt ttagaaccgg gtatttatgc gaaagatcgc cgctgatatt agcatcacct
     6961 gcaacatcag gaatccagat ggtgttaaat cttttgtgct gcaattgtaa ccatccatat
     7021 acaaatggct ctgcaatcac aaacgaagga atcttcacca tcatctgatg tgtgatgata
     7081 actgaatgaa tttttttgtg ataaaatcca tagcgattgt cggatatcac caggtctatt
     7141 tgatgcagcc taacaatctc tttggttgcc cgatgttctc ttctgatgcc actgaatatt
     7201 ttacctagtt gtcccaggat ttttaatatg aaactgccct tttcctggta ggcgatgtcg
     7261 taagcaggca catcaaggca tgtcaattca ggaaattctc ttcgcaacaa tgccagcgac
     7321 cttccggttg aagcgataac taccgtatgg cccctgtcaa cacacgcttt gataatggga
     7381 atactgcgtg tagcatgccc taaaccccag tcgagtggtg ctatgaggat gttaagtggt
     7441 tttggtgctg acaaggcaat attggttaag aaggctacgt ttttactgac gtattgctga
     7501 aatccttaaa ttagagccgc aaagctatga aaaacggtaa aaaactcctc tggttgggcg
     7561 cgataaccat catgctcatt accacttcgg ctacctgtga aggtggcggc aagaagtgcg
     7621 attgccctaa attcagccaa atcggcccga ataacaatga tgccgtggcg agtcaggcgg
     7681 cagcaaatac tttagactga gtataaattc ggcccttaac gaactgtctt cgagcagtaa
     7741 tgtttatcat tgcatataaa gaagacaact atgttaacca aaaaagtaga agcggcactt
     7801 aataaacaag tggaaatgga aggctatgcc tccaattact atttatcggc agcatcttgg
     7861 tgcgattcga aaggattgca gggagctgca ggctttcttt atggtcatgc tgatgaagaa
     7921 cgcatgcata tgatgaaact ctttcattat atcaacgacg cgggcggaca tgccgaagcg
     7981 ccaaatgtga agcagccacc tctgaaattt aagaacctca aggaactgtt taccttggtg
     8041 tttaaacatg aggttgaagt aactaaggcc ataaacaacc ttgtagacct ttgtatgggc
     8101 gaaaaagatc attcttccta caattttctg caatggtatg tagctgaaca acatgaagaa
     8161 gaaaaactct tccgtaccgt gctcgaaaaa attgaattgc tgggtcctga tgataccaga
     8221 ggtttatatt ggatcgaccg cgaactggct gcattaactg ccgctcctag cgcagaataa
     8281 ttgcaatcac acatcattaa agggcatttc tgcaccattc gattagcaga gatgccctta
     8341 atcatgtact tttgtcaaca tgaacacaac tacggtgaat tacctcaata tcgcacttat
     8401 gctgctttca gctatcgtgg cgtttataat tcccttcgaa ttgtttttgt ttagttatgc
     8461 cgttctcgga cccctccact atctgactga aatcggctgg ctgcataagc gcaaatattt
     8521 cagcccgaag aaaaatgatt atataccact ggtgataata accttgctca tagccatccc
     8581 tgccatcatc tcatacatgt ttgtgcacaa cggcgaaaga acttccaccg ggcaattaag
     8641 actcactgaa ggccaacata attttattgc tttctttcag gatcagaata ccgcaacagc
     8701 tttgatgttt ttggcatttt ctatctcgct gatatttatt ttaattcgca acaatacgca
     8761 tagaacaatt gctgttggat tgctggcaat aatcggcttc cttattagaa gtaatcattt
     8821 tgttgatgtg ttattcgcta tgtttctgcc tacgcttatt catgtttttt tgttcaccgg
     8881 agcatttatt ttggttggcg cactgaaaag caaaagcacg tccggatatt tatccatact
     8941 tgtattcctt ggatgttcac tcagcttttt tctaattatg cctgatggtg gaggatatca
     9001 tatcagcgat tacgctaaag gttcttatga tgtatcattc tttggattga atcaccagtt
     9061 gttcagcagc tttctgcatg aaagcaatcc caccaatgat atgatctata attcgtcaac
     9121 aggcattctt attaccagat ttatagccta tgcctatact tatcattatt taaactggtt
     9181 cagcaaaaca tcggtgatca aatggcacga tgtgcctaaa ggatggctgg tgactattgg
     9241 tgtgttgtgg gtaagtgccg ttacattgta ttatatcaat tatgaaaccg gcttgatgtg
     9301 tttgtatttt ttaagctttc tgcatgtggt gcttgaattt ccgctcaact tccagtcatt
     9361 taaacagatt ggtgagcaag taagattgcg gctttccggt aaatccgctg caacgtaata
     9421 tttaattcag atttaaataa ttgactaaat cgcaataaaa caaaaccgcc cgacatgttt
     9481 cagatgcccg ggcggttttg ttttaaaatt atgttcaatt tatgataggg tgccttcgca
     9541 atagtgctta agtagtttca gcgcaatttc catatccttt cccatcattt ttggcagcat
     9601 tcgaccgagt attttcgcaa atccacctcg ctttgttttt ccgcgaacag tccatttgac
     9661 tgaatattgt tggtctttta tttcttcaag actgaaatca aaatccattt tgaaaagacc
     9721 cttgttgaag gcggtggcaa cagttatgtt tttgtatggt tccgttgcaa ctatctccat
     9781 ttttcctgca cccattttct tgcccttcca ctccatgaaa ctgttcacac ctgctgaagg
     9841 attgctgcaa gtgattgtaa tagcaggatc tctgtccgga ctccactgac tccatttcga
     9901 ccaggtttgc aaatcatcga tggtattgaa gatatgtttt ttgtcgccac tgatcacaat
     9961 agatttcgaa aatttatagc tgcgcggaac aaataatccg gcaatgatca acaacgcgaa
    10021 aattcctgca atgactaata aaattgttcc tatcataact cgccgctatc cgaaatattg
    10081 aataattata tattaattaa aagcgatatc ccaaacaaat accaaacacg cgcgcaccta
    10141 aaaacaatcc gcttaaatcc gcatctacaa cgcccaatcc tgtagttcca ttatcagtaa
    10201 cagtggcatc gatattccaa cccggaattg gcaccgattc aatatcgttt tcgagtttga
    10261 ttttatcttc aggtgtgata ggcctgtcgt catcgccgtg tgatttacct gtttgatatc
    10321 caatgaaagg acctagtatc caccaatcca atgtcatgct gtttcctatt ttccattgca
    10381 cacccaccat ttcgccaaca ccaacaccat taaatgtagt ttcaacatcc ggatggtgtt
    10441 cgccatcgct taaattaaag gtgtaaatct gacgatgtgt catatgtgta tagcgtataa
    10501 atgaagcacc atatacacca aatggagctc cttttacttc actgtaaaag cgatactccg
    10561 gtgtgatgct gaaagtattt aactgcgtgc tgttaatagc ggtgatcgcc tgatcattcc
    10621 cttcaaaaat attaacgagt gcgtttttga aaggcaggtc catattaggc gcaaaactga
    10681 tagacatgcc aaccgattgt tttacattta atacatgctc gtactggagt gtataattcc
    10741 cgaatacagc accggaaaga ttaaatttga tgatatccgg tttgtcgaga ttgttgggaa
    10801 tggtatttgt tgtttgtgca tgtaacatca tgccgctcgc tacgaagagc attgttgcga
    10861 agaatttacg tatgtgcata ggagtgagtt ttatagttaa ataattatca atttgaaaaa
    10921 agcaagttag ttcattcctt aaagatagtc atttgcccat gggaaagtaa taactaaaat
    10981 gcgtgattat tttaatttat ttttcagatg ttgcccggta tagctttcct taactgttac
    11041 tattccttcc ggagccccct gataaagtag gtatccacct ttgtcgccgc cttcaggacc
    11101 gaggtctatc atataatcgg cgcattttat cacctcgaga ttatgctcaa taaccaagac
    11161 actatgtccg cactcgatca gtgcattaaa ggcatccatt aatttattga tgtcgtggaa
    11221 atgcagaccg gttgtcggct catcgaaaat aaataggata tgctccttgc ttttcccttt
    11281 accgaggaaa ctggcaagtt ttaccctctg cgcttcgcca ccggataaag tgctgctgct
    11341 ctgtccgagt tttacataac ctaaacccac atcggcaagc ggctgtagtt tggcgacaat
    11401 atctttttcg tcttgcaaga aatgtatagc ttcctctaca cttaatccta gtaattcaga
    11461 gatatttttg tttttatatt gtacctccag aatttcttcc ttaaagcgct tgccgttaca
    11521 atcttcacat accaaatgca catcagcgag gaattgcatt tcaacaataa tctctccttc
    11581 ccccttacac atttcgcatc ttccaccgtc gacattgaaa ctgaaaaatc cggatttgta
    11641 cccgcgcatt tttgcaagag gctggcgcgc atataaatcg cgaatctcat cataggcttt
    11701 tacataagta accggattgg agcgtgatga ttttcctata ggatcctgat caaccaattc
    11761 aacttgtgtg attgcgctca catcgccttc aatcactctc gaaataaatg ctgtgtcact
    11821 cgcatcatcg agcatcttca tcaattcggg atataaaata tgtttgatca atgtagtttt
    11881 acccgaaccg ctaacacctg aaactgcaac taaattctgc aatggaatag taacatcaat
    11941 ttgtttcagg ttgtgcatgg acgcctgacg aatgcgcaat ttcttaaaac tctttctgcg
    12001 tgttcgaggt agcggaattt gcatggttga cgacaaataa tttcctgtca ggctttcttt
    12061 atcggccaat atctcttcca gatttccttc gctcacaact ttgccgccat gaatgcctgc
    12121 agcaggtcca atgtcaataa tatgatccgc attgcgaatt acatcttctt cgtgttcaac
    12181 aacaaccacg gtgttgccga gattgcgcaa gcgttttaaa acggttacca atcgctcagt
    12241 atcgcgtgga tgtagcccga cacttggttc atcgagaatg tatagtgatg acgttaaatt
    12301 actgccgagc gttctggtga gattgatgcg ttgtgtttcg ccaccgctca atgtgttgct
    12361 tcttctgtta agtgtgaggt atccgagtcc gaggtctaac atataaacta tccggtttcg
    12421 aatttccaac agaatgcgtg aggcaacttt tgtttcgtat gcatttaatg tgatgtcatt
    12481 aataaataca gccagatctt ctacttgcat gtctacaagt tttgtgatcg acatattatt
    12541 aattaataca taatctgcat cgggacgaat tcttgaacca ttgcattcag gacatgttgt
    12601 ttttccgcga tagcgtgcaa gcattacgcg atattgaatc ttgtatgcat tttcttccaa
    12661 cattttgaag aatcgatcga gtccccagaa atgttcattg ccagtccaca gtaattgata
    12721 ttgcgctttt gttaattgcg aaatgggttt gtgtatgggg aagtcgaatt tctttgcagc
    12781 tttcaacaag tcgtcgagcc aggtgcgcat tttctcgcct ttccaacacg caacagctcc
    12841 ctgatatacc gatagatttt tatcaggaat aaccaagtca tgatcaatgc cgataacatt
    12901 gccaaatcct tcacaacgct tgcaggcgcc atagggatta ttctgattga aaaaatgtgg
    12961 cgatggcact tcaaactgca taccgtcagc ctcgaaatta ttactgaaac tgcgttcttc
    13021 aattccttca gctaccacat gacaggcgcc atgtccttcc gaaaaagcaa tatcaacgga
    13081 atcggcaatg cgttgacgag tctcctcatt cacttccgga acatttgaac cttcagcgcg
    13141 ctctttagcc aacactacgc gatctatcag cagatgcacg ggtgttttcg gtgcgatcag
    13201 ctttggatct tcgagtatat cctcaatttt tacaatgctt ccgttggcat aaaagcgcgt
    13261 gaaacctttt tgctgtaata ctctggcctg atcgttgagc ggtcggtctt tgtctacatg
    13321 caatttgact aaaaggtata tcctactgtt ttctggaagg gagcagatat aatctacaac
    13381 atcatttact tcgtcctttt tcacctctcg ccctgaaatt gggctatagg tgcgtcctac
    13441 gcgcgcatac agtaggcgca tgtagtcgta aatctccgtc agggtgccaa cagtactcct
    13501 ggtggtgcgt gtagtaacct tttggtccag ggctattgcc ggggcaattc ccttgatata
    13561 atctacatcc ggcttgtcca ggcgattgag gaattgtctg gcataggcgc tcaaactctc
    13621 tacgtaccgg cgttggcctt ctgcatagag ggtatcgaag gtaaggcttg acttgcccga
    13681 tccgctcaca ccggttacca ccaccaggcg gttcctgggt atggcaacat cgatgttttt
    13741 gaggttgtgc tcgcgggcgc cttttataaa tattgcgtca atatgaccgt ttggatagct
    13801 ttttttcgtt ttcaaacccc ttttcttgaa acttttttaa taaaatatcg taaatcttaa
    13861 ccaattccct ttgaaaatat caaatgaagt gtatatttgt tgtgcaaatc tcaccaaaat
    13921 tagctgccta taaggttaaa tctacgcgaa aagaatagtt tattcattta acgcacaacc
    13981 ttttagtatg cagtccagaa ccctttctga tgacaagctg gtcgaattgt acctgaccgg
    14041 cgaagaatct gccctcggtg aactcctcaa ccgtcacaaa gataggatat acacgtacat
    14101 ctatcttttt gttcgcgaca cacacactgc tgaagacctc ctccaggaaa ccttcatcaa
    14161 ggtgattgac aagctacggt ccggaaagta tcaggaacag aacaaatttt tgccatggat
    14221 atctcgcgtg gcgtataacc tttgcataga ctactatcga cgtgaaaagc gcatgcccaa
    14281 agtggttacc cgcgatggct tcgatatttt cagtgtgctg aagttctcag aacccggcgc
    14341 agaaggcaaa gtgatggaat cggaattcag cagccgcgtc cgcaaattgc ttgaatacct
    14401 gcctgaggaa cagcgcgaag tggtggtttt gcgccattat atggacatga acttccgcga
    14461 aattgccgaa atgacggggg taaacatcaa tacagccttg ggacgtatgc gctatgcact
    14521 catcaacctg agaaaactga tggaaacgaa aaatattagc atttgatata ctaacgcaat
    14581 ctgcatgaac gttttaggtc tgttctttta tggttaaacc taaaacaaat tgcatgatta
    14641 aaaccattac acaagacaat ctcattcgat ttatctatcg cgaaacaaca gcagaggaag
    14701 ccagcctcat tgaagaggcg ctgcgcagcg acttcgatct gaaggaactg tatgagcaaa
    14761 tgctcgaaag caaggctgaa ctcgataagg tccgactgca accttcgcaa aaagtgatcg
    14821 ataacatcat gaactacagc aagaactccg ctcacatgga gcacctgctg taaaacgaga
    14881 tcaaaaaaaa cccgatctga ccagatcggg gttttttatt tattaatatt ttcactcacc
    14941 acccgcctat cggcgaggca agctcaccca ccc
//
LOCUS       DAOQOU010000066        14891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2041_length_14891_cov_5.663252, whole genome shotgun sequence.
ACCESSION   DAOQOU010000066 DAOQOU010000000
VERSION     DAOQOU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2041_length_14891_cov_5.663252"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5586)
                     /gene="sprA"
                     /locus_tag="PLM90_07380"
     CDS             complement(<1..5586)
                     /gene="sprA"
                     /locus_tag="PLM90_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMZ89230.1"
                     /translation="MAVAKFFPQIILGTAVSVTVMSGILRNDPQLVADHAASDTYFFS
                     PAAPIDTPPSLHYPFPDDDGSNPFQNDENNFDLEPTQQPTIIYDSETNEYLIQNEIGG
                     DFIENPQYITFEDYVNAQEDQAIDDYWKERSDASNILGGTGGKIELVPFDRECAIFDG
                     CNVEIRPQGNVELTFGGSFQNIQNPILTESQRRQGGFDFDMDINANVLGSIGNNLKLN
                     FNYNTQATFDFENQIKLEYKGKEDNIVQGIEAGNVSMPLTTQLIPGPTSLFGIKTELQ
                     FGRLTMTNILSQQKSQTQSIQIEGGAQKREFEIFADQYDENRHFFMSHYFRKGYEGWL
                     KTLPYVSSPINVTKIEVWVTNTTGETQNTRDIVAFSDLGEPDSIYWGEIDPAGTVSSL
                     TPGGLPSNNANNLYQKLYGTAGTRDLNTVISTLTGPAFNMQAVRDYEKARMRKLDPTD
                     FIFNPQLGFISLNTTLQPDDVLAVAYEYTYNGQTYQVGEFSSDLPPGVDTSNVLFMKM
                     LKSTSAITQIPLWDLMMKNVYSLGAFQVSSDGFRLDVVYQDPGGGMKRFIPANNSNVN
                     STPLIKLLGLDRLNNNNDPQPDGIFDFVNGVTINTQNGRVYFPVLEPFGGYLRSDAVF
                     GNDLEAEKYAYDELYNETKTIALQFPQYDRYVLSGEYKSSVSSEIYLGAFNLPKGSVK
                     VTAGGQLLTENVDYTIDYSLGRLKIVNEAILNSGQQINVTFENNAFYGFQTKSLVGSR
                     LDYWINDNFTLGATWMHLSERPYTQKVNIGDDPISNSIYGFDINYTNDAPWLTRALDK
                     LPFYGTKETSTISLTGEVARFQPGHSKAIGKDEAGRIYIDDFEGSRSAYDLKFPYSSW
                     VLASTPQGATDENNAILFPEAGLFDSLTYGMNRAKLAWYNIDPLFNEQNNPSTPDHLS
                     DADLSNHYTIQIDEKEIFPQADYETTVLTQLSTFDLAYYPTERGPYNFDLGGTPFSAG
                     VENDGSGKLREPETRWGGLMRNLETNDFEAANIEFIEFWVMDPFDNRGPFGTQVTSDG
                     YLYINLGNVSEDVLKDSRMFFENGLPKDGSTDALDQTNWGNVPRTPPITTAFDNDVES
                     RANQDKGYDGLNSDEEADFYNQFMLDAPTYVSGAALVKLESDPSTDDYHYYRGGDYDD
                     AELSILERYKKYNNPQGNSPTTESSPEAYPTAATTIPESEDLNDDYTLNETESYFQYR
                     IKLEPGMESINPYITDVVVAEHTFQDGSSDSIKWYQFKVPIDQFDGKVGSIQDFKSIR
                     FMRMFMTGFTDDVVLRFARLELVRNQWRRYQFSMLNPGEYIGNDDGNETDFNVSSVSI
                     EENSARQPIPYALPPGISREQTLGSGSSVSTYQQNEQSLSMQICPLQDGDARAIFKSL
                     NIDLRRYGRLTMNVHAEPLPDAPLTTLNDGDLHIFIRLGSDFTNNYYEYDLPLTVTKL
                     PLPAGTTEDEAVREAIWATLIDFPLDSLVQVKTLRNKNEVSPLVPFSITSGTDQGTYT
                     IIGNPDLGYVEEIMIGVRNPKDIGGSGAEFCSEVWVNELRLSNIDESGGWAALARADV
                     KLADLGNFTLSGNLHTAGFGTLEQQIDERYKDNFYQYAASLSLELGKFLPQGVNLRLP
                     LYASISESFSNPEFDPYELDVDLQQKLDEISTTYGADSSDLYKRSVIDYIAVKSINLS
                     NIRFERGDKAGDPHLWDKENFDLTLAYSQEYNSNPILEYDIVDRYKAALGYSYSQKAK
                     YILPFEKVIDKKYKWLTPVRDFNFNLLPTGYSFRTELNRQYGETLMRDVYGDGLIDPT
                     YDKYFTWDRFYGLKLEPAKSIKIDFSATNNARIDEPYGALDTKEKRDSLWNSFYDFGR
                     NINYRHTVSATYTLPISKLP"
     gene            complement(5597..6184)
                     /gene="ruvA"
                     /locus_tag="PLM90_07385"
     CDS             complement(5597..6184)
                     /gene="ruvA"
                     /locus_tag="PLM90_07385"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMZ89231.1"
                     /translation="MIAYLSGQFTVKNPAYMVVECNGVGYQVHISLATYSKIQGMEKG
                     RIHTYMVVREDAQLLFGFADETEKNMFVNLLSVQGVGPNTARMILSSMNVDELEQAIL
                     SENDPLIQKIKGVGPKTAKRIVIDLKDKVVKGSTGTENSFGASHNTIKEEALIALAML
                     GFSRQQGEKAIAAALKNSPNPVDVENLIKLALKNL"
     gene            complement(6450..8735)
                     /locus_tag="PLM90_07390"
     CDS             complement(6450..8735)
                     /locus_tag="PLM90_07390"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMZ89232.1"
                     /translation="MPEKLRKKDALDYHSSGRPGKIEVIPTKSTKTQRDLSLAYSPGV
                     ADPCLEIAANQEDVYKYTAKGNLVGVISNGTAVLGLGDIGPEASKPVMEGKGLLFKIY
                     ADIDAFDIELNCRDVDEFVRIVKAMEPTFGGVNLEDIKAPECFEIEKRLKAEMRIPVM
                     HDDQHGTAIIAGAALLNAIEILGKDLSTIKVVYSGAGASAISCARLFVQLGVTRENIT
                     MLDSQGVINAQRDGLDEYKSQFISNTTATTLAEAINGADVFVGLSKGNILTPEMLLTM
                     AKDPVVFALANPDPEIAYDLAVATREDVIVATGRSDHPNQVNNVLGFPYIFRGALDVQ
                     ATEINEAMKVAAIRALAALAKEPVPEFVNMAYGEKNLMFGRGYIIPKPVDPRLITRVA
                     PAVAKAAIKSGVARRTITDWEEYEHELSNRLGLEQQLIKAITSKAKQNPKRVVFAEAD
                     NYKILKAAQIVREEGIAKPILLGPIEKIKRIIEENQLDLRNVPIIDTRSTDQEETRAR
                     YGEIFFNKRMRRGVNLYEARKMMRDRTYFGAAMVETGDADALISGLTRQYPETIKPAL
                     QIIGREDGSKKVAGMHIVMTKHGPVFFADTTATFNPTAEELVEITALTAKAVRQFNIQ
                     PKIALVSYSNFGSVENDETLVLRNAVALLKERFPGMIVEGEMQVNIAFENELMKENYP
                     FSALADKSVNTLIFPNLHAANIGYQLMQSLGQSEVIGPILLGMKRSVHILQLGSSIRE
                     IVNMVAIAVTDAQVKSKRKAL"
     gene            9347..10282
                     /locus_tag="PLM90_07395"
     CDS             9347..10282
                     /locus_tag="PLM90_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89233.1"
                     /translation="MATVTFKKIVYLHSGTGVRHHHYSSSEGIISGPVNSNYPGTTDT
                     TSGTYSADGAAQEIYNGQLVNFAFMAVKGSADGNQLFTSAGDQSIRVGSNNVTVLVVY
                     APPGGIGVNGGPGVWVDAFNVDTCAFSDDLHFIQVFTPPTPPDNLDAAKTDFANMEGE
                     ISTVTAEHMRASTTVDGAIPFLEWKQVTSNDSPVGSRDVDLAQGQSGQIWFAFYKTPP
                     APPAPSIPHFNDRLGWIYLSPGVLVGGGGFGIGPDGKPHPIDPDGPLMQQLLSTFAMI
                     SVSANMHEKLQMEARRIASDNLRMIADSVQNKRLK"
     gene            complement(10345..12417)
                     /gene="scpA"
                     /locus_tag="PLM90_07400"
     CDS             complement(10345..12417)
                     /gene="scpA"
                     /locus_tag="PLM90_07400"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HMZ89234.1"
                     /translation="MSKRSLRNIPYHHYQLPSVQVQPDAGFDVVKKSLPGSAPFLRGP
                     YSTMYVMQPWTIRQYAGFSTAEASNAFYRKNLAAGQKGLSVAFDLATHRGYDSDNPRV
                     FGDVGMAGVAIDTVEDMKILFDQIPLDQMSVSMTMNGAVIPIMAFYIIAAEEQGVTPQ
                     QLAGTIQNDILKEFMVRNTYIYPPVSSMRIVADIFDYCAKNMPKFNSISISGYHMQEA
                     GATPEIELAYTLADGVEYVRTGIAAGMQVDDFAPRLSFFFGIGMDHFMEVAKLRAARV
                     LWCRLMEQFAPKNNKSLMLRTHCQTSGWSLTEQQPFNNVARTCIEALAAALGGTQSLH
                     TNSLDEAIALPTDFSARIARNTQIYLQKETGICDTVDPWGGSNIVEALTDKLIHDAWK
                     LIQEVENLGGMQKAIEAGIPKMRIEEAAAKKQGRIDSGTDVIVGLNAFKTEEENNIDI
                     LEVDNSAVRNAQIERLHEVRNSRDNNAVKQALAKIEDAAKTGNGNLLALAVDAARIRA
                     TLGEISDAMEAAFGRYTAETRTIAGVYQKEVMQNETFKQARNLSDAFAGKAGRRPRIL
                     IAKLGQDGHDRGAKVIATAFADIGFDVDISPLFLTPEEAVKQAIENDVHIIGISSLAA
                     GHKTLVPRVIDLLKEQGREDMLVVVGGVIPQQDYAFLEEAGVAGIFGPGTVIADAAIT
                     LLQRLMTE"
     gene            complement(12420..13622)
                     /locus_tag="PLM90_07405"
     CDS             complement(12420..13622)
                     /locus_tag="PLM90_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ89235.1"
                     /translation="MDELINNDIEKAPWLQQVTKDLKGADFREKLVHIKEGIEIMPLY
                     TASDLPEFEPDANADFLADMDDDFAAEVGNQHQQHLTWNITEDWDVSASTNMHELVQQ
                     ARQRGVIDLRLHAEVDSDYDAIFDGIRKMGSPANLHFDATGPINEHTAGSWRERIGFL
                     GDRDRLIQSIQFDPISYWEKNGYPASTNAQFDRLADAILRINGHLQDCRLIKVDTTDL
                     ADAGASSIDQLVKALHRTAVYFEEMEKRNLSLFEITQLFTFRFGVGTNYFFEMAKLRA
                     FKILWNNLVKAYDPELDFIPTPYIHAVVSTHSFTQEDKHSNLLRSTTAAMSALLGGVD
                     ALSIPTFDLQQDAQSVRLAINIQNLLRYESYMDRYRDAANGSYYIESLTRQFGEKAWS
                     IFIETHNA"
     gene            13718..14641
                     /locus_tag="PLM90_07410"
     CDS             13718..14641
                     /locus_tag="PLM90_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89236.1"
                     /translation="MKTVYRKLEAYSAAAIALMAVKTAEAQVHYADVDPDIVLDSPDA
                     FALIDLNGDGLADIKFANQSFYLLLWTFYSEEKPYLFQYINASAYAELFAYNENSVAY
                     TGSEGGLSGGVWHVAKAIEEGEVVNAELDFEEWGVPMAEKIYSCTLTAGFGATSCVFS
                     DSVGKWQPELANRFIGLHFTNITDIGYFGWIRCSIANDGRKLTIHDYAYNATPEQAII
                     AGDTLAVGISEATLQGTQMYTDGNNVLHAKTTSSGNTRLQLFDISGREIVFLQNNIGE
                     FNYDLTALPAGPYIVRLTQGASSSTIKIIRK"
     gene            14638..>14891
                     /locus_tag="PLM90_07415"
     CDS             14638..>14891
                     /locus_tag="PLM90_07415"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89237.1"
                     /translation="MNAWIKPAILTTCFICSNKLQAQWSTVDSTFSDDGIAIISAGAG
                     NDRCYTSAVQSDGKLIIVGNSIYESGGYFFFISRLFSDGTP"
BASE COUNT         4047 a         3191 c         3110 g         4543 t
ORIGIN      
        1 tggcaatttg ctgataggta aagtataggt agccgaaacg gtatggcgat aattaatatt
       61 tcttccaaaa tcataaaaac tgttccacaa tgaatcgcgt ttttctttgg tatctaatgc
      121 accataaggt tcatcaatac gcgcattgtt agttgctgag aaatcaattt ttatcgattt
      181 ggcaggttct aatttcaaac catagaaacg atcccatgtg aaatacttgt cgtatgtagg
      241 atcgataagt ccatcgccat aaacatcgcg catcaaggtt tctccgtatt ggcggttaag
      301 ctctgtgcgg aaactgtaac ctgttggcag cagattgaaa ttaaaatcgc gaacgggagt
      361 cagccatttg tattttttat cgataacttt ttcgaagggc aggatatatt ttgccttttg
      421 actatagctg tatcccaatg cagctttata tctgtcgaca atatcgtatt ctaaaattgg
      481 attgctgtta tattcctgag aatatgccaa tgtgagatcg aagttctctt tatcccacaa
      541 atgcgggtca cctgctttat caccacgttc aaagcgaata tttgaaaggt tgatactttt
      601 cactgcgatg taatcaatca ccgaccgttt gtatagatct gaagaatcgg caccataagt
      661 ggtgctgatc tcatctagtt tttgttgcaa gtcaacatcc aactcgtaag ggtcaaattc
      721 aggattactg aatgattcag aaatgcttgc atacaatggt aaacgaaggt tcacaccctg
      781 aggcaggaat tttcccaatt ccaaactcaa tgaagcggca tactgataga aattatcttt
      841 atatcgttcg tcaatttgct gctcgagggt tccgaatcct gcagtatgta aatttccgga
      901 gagagtaaaa tttcccagat cagccaactt cacatcggcg cgagccaatg cagcccagcc
      961 gccactctca tctatattcg ataaacgcaa ttcattcacc caaacttctg aacagaattc
     1021 agcaccgctt ccacctatgt ctttcggatt gcgcacacca atcatgatct cttcaacata
     1081 tccaagatca ggattaccga tgatggtata tgtgccttga tctgttccgc tggtaatact
     1141 gaaaggaacc agtggcgaaa cttcattttt gtttcgcaat gtttttacct gcaccaaaga
     1201 atccagcggg aaatcgatga gtgttgccca aatggcttct cgaactgctt catcttcagt
     1261 ggttcctgca ggcaatggta atttagtaac cgttaacggt aaatcgtatt cgtaatagtt
     1321 attggtgaag tcggaaccaa gtcggatgaa gatatgcaga tcaccatcat ttaatgttgt
     1381 gagcggtgca tccggtaatg gctcggcatg cacgttcatg gttaggcgac catagcgacg
     1441 aagatcgatg ttcagcgatt tgaaaatggc gcgcgcatca ccatcctgta atggacagat
     1501 ttgcatgctc aatgattgtt cattttgctg ataggtagat acagaacttc cgcttcctaa
     1561 tgtttgctca cgggaaattc cgggaggcaa tgcataaggt attggctgac gtgcagagtt
     1621 ttcttcgata cttacacttg atacgttgaa atcagtttca ttcccatcat cgttaccaat
     1681 atattctccg ggattcaaca tagaaaactg ataacgacgc cattgattgc gcactaattc
     1741 taatcgtgca aatcgcaata ccacatcatc ggtaaatcct gtcatgaaca tacgcatgaa
     1801 acgaatagac ttgaagtctt ggatacttcc aaccttaccg tcgaattgat cgataggcac
     1861 tttaaattga taccatttga tagaatcgct gctgccatcc tggaaagtat gttcagctac
     1921 cactacatct gtgatatatg gatttattga ttccattccc ggttccagct taatgcgata
     1981 ctggaaataa ctttccgttt catttaatgt ataatcatca ttcaaatctt ctgattccgg
     2041 aatggtagtt gctgcagttg gatatgcttc aggagatgat tcagtagttg gtgaatttcc
     2101 ctgagggtta ttatattttt tataacgctc aagaatactt aattccgcat catcataatc
     2161 accaccgcga tagtaatgat agtcatccgt tgatggatcg ctttccaatt taaccaatgc
     2221 ggcgcctgaa acataagttg gtgcatccag catgaattga ttataaaaat cagcctcttc
     2281 atcactgttc aaaccatcgt aacctttatc ctgatttgca cgtgattcta catcattatc
     2341 aaatgcagta gtaattggtg gtgttcttgg aacattaccc caattagttt gatccaacgc
     2401 atcggtgctg ccatcttttg gcaaaccatt ttcgaagaac atgcgcgaat ctttcagtac
     2461 atcttcggat acgtttccta aattgatgta gaggtagcca tcactggtta cctgtgtgcc
     2521 gaaaggtcct ctgttgtcga aaggatccat cacccagaat tctatgaatt caatattcgc
     2581 agcttcgaag tcgttggttt cgagattgcg cattaagcca ccccagcgtg tttcgggttc
     2641 acgaagtttt cctgagccat cgttttcaac acctgcagaa aatggtgttc cgcctaaatc
     2701 aaaattataa gggccgcgtt cagtaggata atatgcaaga tcaaaagtgc tgagttgtgt
     2761 caacacagtt gtttcgtaat cagcttgagg aaaaatttct ttttcatcga tctgaatggt
     2821 atagtgattt gaaagatcag catcacttaa gtgatcaggt gttgatggat tattctgttc
     2881 gttgaataaa gggtcaatat tataccatgc taattttgct ctattcattc catatgtaag
     2941 actatcgaac aaccctgcct cagggaataa gatagcatta ttttcatctg tagcaccctg
     3001 cggtgtactg gccagtaccc agcttgaata agggaatttc aaatcatacg cacttctgct
     3061 gccttcgaag tcgtcgatat aaattctacc tgcttcatcc ttgccgattg ctttactatg
     3121 tcctggttgg aaacgtgcaa cttcacctgt caatgaaatt gtagatgttt cttttgttcc
     3181 atagaacggc aatttatcca acgcacgagt cagccagggt gcatcattag tgtaattgat
     3241 atcgaaacca taaattgagt tggagatagg gtcatcgcca atatttactt tttgggtgta
     3301 tggacgctct gataaatgca tccaggttgc acctaatgta aagttgtcgt ttatccaata
     3361 atctaatcgc gaaccaacga gtgatttagt ttgaaaacca taaaaggcat tattctcgaa
     3421 agtaacattg atctgttgac ctgaatttag tatcgcttca tttacaatct tcaaccttcc
     3481 taatgaatag tcgatggtat agtcaacatt ttctgtcagt aattgaccgc ctgcggtaac
     3541 ttttaccgac cctttaggta agttgaatgc accgagataa atttctgacg acacgcttga
     3601 tttatattct cccgacaaca catatctgtc gtattgcgga aattgcaatg cgatagtttt
     3661 tgtttcgtta tacaactcat cataagcgta tttttctgct tccagatcgt ttccaaaaac
     3721 tgcatcactt cgcaaatagc cgccgaatgg ttccagtaca ggaaagtaca cacgtccgtt
     3781 ctgtgtgttg atggtgacgc cattcacgaa gtcgaaaata ccatccggtt gagggtcgtt
     3841 gttattattc aaacggtcga ggcccaataa tttaatcagt ggtgtgctgt taacgtttga
     3901 attatttgcg ggaatgaatc gcttcatacc tccacccgga tcttgataca caacatcgag
     3961 gcggaaacca tcgctgctca cttggaatgc accaagagag taaacgtttt tcatcatcag
     4021 atcccacaat ggaatttgag taatcgccga tgtgctcttc aacattttca tgaatagcac
     4081 attgctggta tctactcccg gaggtaaatc gctcgaaaat tcacctacct gatacgtttg
     4141 tccattgtaa gtatattcat aagcaacggc caaaacatca tccggttgta atgttgtgtt
     4201 cagtgaaatg aaacctaatt gcggattaaa tataaaatct gtagggtcga gtttgcgcat
     4261 tctcgctttt tcgtaatcgc gcactgcctg catgttaaat gccgggcctg taagtgtgct
     4321 gatcactgta ttcaaatcgc gagtgcctgc ggtgccatat aatttctggt agaggttgtt
     4381 ggcattattt gacggcaaac caccgggagt cagagaactt actgttccgg caggatctat
     4441 ttcaccccaa taaatactat ccggttcacc aaggtcagaa aaggcaacaa tgtcgcgcgt
     4501 attttgtgtt tcacctgtag tattggttac ccaaacctca attttagtta cattgatcgg
     4561 tgagctgaca tatggcaatg tcttcaacca tccttcatat cctttgcgga aataatgcga
     4621 catgaagaag tgacggtttt cgtcgtattg atcggcaaaa atttcaaatt cacgtttttg
     4681 tgcgccgcct tctatctgga tgctttgtgt ttgagatttt tgttgcgata aaatattggt
     4741 catggtaagt cgaccaaatt gcaattctgt tttaatacca aacaaagaag tagggcccgg
     4801 aattaattga gttgtcaaag gcatgctcac gttaccggct tcaatacctt gaacgatatt
     4861 atcttctttg cctttgtatt cgagtttgat ttgattttca aaatcgaatg tagcctgtgt
     4921 gttatagttg aaatttaatt tcaggttatt gccgatagat cccaaaacat tcgcattgat
     4981 atccatatcg aaatcaaaac caccctgacg tcgctgactt tcagtgagga taggattctg
     5041 gatattttga aaacttccac caaaggttaa ctcaacgtta ccctgagggc gtatttctac
     5101 attacaaccg tcgaatatgg cacattctct gtcgaatggg acaagttcta tcttacctcc
     5161 ggttccgccg aggatgtttg atgcatctga tctttctttc cagtaatcat caattgcctg
     5221 atcttcctga gcatttacgt aatcttcgaa ggtgatatac tgtgggtttt cgataaagtc
     5281 gccgccaatt tcattctgaa tcaggtattc gttggtttca gaatcgtaaa ttatggtagg
     5341 ttgctgagtc ggctcgaggt cgaaattgtt ctcgtcattt tggaatggat tagacccgtc
     5401 gtcgtcaggg aaagggtagt gcagcgatgg aggagtatcg attggtgctg ccggactgaa
     5461 aaaatacgta tcagaggctg cgtgatccgc aaccaattga ggatcatttc gcagtatacc
     5521 cgacatcacg gttacactaa ctgctgtacc taaaattatc tggggaaaaa atttcgcaac
     5581 tgccaaaacc tcttgtttac aagttcttca aagctaattt aatcaggttc tccacatcaa
     5641 ccgggttcgg actgtttttt agggctgcgg cgatggcttt ttcaccctgc tgcctactaa
     5701 atccgagcat cgccaaggcg attaacgctt cttctttgat ggtattgtgt gatgcaccaa
     5761 aagaattttc agttccggtc gatcccttca ccaccttatc tttcaggtca ataacaatgc
     5821 gttttgcggt tttaggtcct acgcctttga ttttctggat cagcgggtcg ttttccgaca
     5881 atattgcctg ctccagttca tctacattca tgcttgaaag tatcatcctg gctgtgtttg
     5941 ggcctacgcc ttgcacagaa aggaggttta caaacatgtt tttttcggtt tcatctgcaa
     6001 agccaaagag cagttgggca tcttcacgca caaccatata ggtgtggatc cgtccctttt
     6061 ccatgccctg tatcttcgaa taagtagcta aactgatatg cacctgataa cccactccgt
     6121 tgcattccac aaccatgtag gcagggtttt taacggtgaa ttgtccggat aaatatgcaa
     6181 tcatgtcgca gtggatgagt tttcagtatg gatatttctg caccacaact acggcaaatg
     6241 tttgaaacat agccatttag tacatgcaga gggggcaaat ttcgtggaaa ttataggaat
     6301 ttgaaagcct tatggaaaat aaggtgcaac ctaatttatg gtttgacttt tatatgtttc
     6361 aaacgccttc aagacaaacg cataattccg taaaaccaat tgccggaatt gatccgaaat
     6421 gctcagtgta aattgcctga aacccttgat tacagtgcct ttcgcttcga tttcacttgc
     6481 gcatcggtaa cggcaatggc caccatattc acaatttccc tgatagagct tcccaattgc
     6541 aggatatgta ctgatcgctt catacctaac aaaataggac cgattacctc cgattgtcca
     6601 agagattgca tcaattggta gccgatatta gcggcatgga ggttcgggaa aataagggtg
     6661 ttcacgcttt tgtccgcaag ggcactgaaa ggataatttt ccttcatcag ctcgttctcg
     6721 aaagcgatgt tcacctgcat ttcaccttct actatcatac ccggaaaacg ctccttcagt
     6781 aaggctacgg cattgcgcag cactaaggtt tcatcgtttt caacagatcc gaagtttgaa
     6841 taggaaacca gcgctatctt aggctgaatg ttgaattggc gaacagcttt tgcagtcaga
     6901 gcggttatct cgacaagttc ctcggccgtt gggttgaagg tggcagttgt atcggcaaaa
     6961 aataccggac catgtttcgt catcacgata tgcatcccgg caactttttt cgagccatct
     7021 tctcttccaa taatctgaag tgcaggttta attgtttcag gatactgacg cgtcaatccg
     7081 gagatcaagg catctgcatc tcctgtttct accattgcgg caccgaaata cgtgcgatcg
     7141 cgcatcattt tacgcgcttc gtaaaggttt acaccgcggc gcatgcgctt gttgaagaag
     7201 atttcaccat agcgggcgcg cgtttcttcc tgatcggtgc ttcgggtatc aatgataggc
     7261 acattgcgca aatccagctg attttcttcg ataatgcgct tgattttttc gatcggaccg
     7321 agcaaaattg gcttcgcgat gccctcttcg cgcacgattt gggctgcttt cagaatttta
     7381 taattgtcgg cttccgcaaa tactacgcgt tttgggtttt gttttgcctt cgaggtaatg
     7441 gctttaatga gctgttgttc caatcccaat ctattcgaca attcatgctc atattcttcc
     7501 caatcggtga tggttctgcg ggcaacacct gattttatgg cggccttagc aacagcaggt
     7561 gcaacacgtg taatcaatcg cggatctaca ggttttggaa tgatataacc tctgccgaac
     7621 atcagatttt tttcgccata agccatgttc acaaattccg gaaccggctc cttggcgagt
     7681 gctgccaatg cccttatagc ggccactttc atcgcttcat tgatctcagt cgcttgtacg
     7741 tcaagtgctc cgcgaaagat atatgggaat cccaaaacat tgttcacctg attaggatga
     7801 tcggaacgtc ctgttgcaac gatcacatcc tcgcgggtgg ctacagcaag atcatatgct
     7861 atttccggat cggggtttgc aagtgcaaat actaccggat ctttcgccat ggttaacagc
     7921 atttcagggg taagtatatt ccctttcgac aaaccaacaa aaacatcggc accgttgatg
     7981 gcttctgcca atgtggtggc agttgtattg gaaataaact gactcttata ctcatcgagt
     8041 ccatcgcgtt gtgcattgat gacaccctgg ctgtcgagca tggtgatatt ttcacgcgta
     8101 acaccgagtt gaacaaataa acgcgcgcaa ctaattgctg aagcgcctgc gccggaatac
     8161 acaactttga tggtgctgag gtcttttcca agtatttcta tggcattcag caatgcagct
     8221 ccggcgataa tagcagttcc gtgctgatcg tcatgcatca ccggaatgcg catttctgct
     8281 tttaatcgtt tctctatttc gaagcattcg ggtgccttga tatcttccag gttaacacct
     8341 ccgaatgttg gctccatagc cttgactatg cgcacaaatt catctacatc gcggcaattc
     8401 aactcaatat caaaggcatc gatatcggca taaatcttga atagcaggcc ttttccttcc
     8461 attacaggct tagatgcttc aggtccgata tcgcccagac caagaacggc agtgccattg
     8521 ctgatgacac caaccaggtt tcccttggcg gtatatttat atacatcctc ctgatttgcg
     8581 gcgatctcta aacaaggatc agctaccccc ggagagtatg ccaaggacag gtcgcgctgt
     8641 gttttagttg atttggtggg gatgacctca atcttaccgg gacggcctga ggagtgataa
     8701 tcgagggcgt ccttcttgcg taatttttct ggcattgaaa aggctgttga accaacaaag
     8761 gtagtaataa cagaacaagt gtatatcata gacctgaaat tgctaactcc tatgccaatt
     8821 tttagttgtg gcgcgaaagt gtaaagtcga tgagttaata aaaatttaaa attgaaaaag
     8881 ttgctgctgt caaagtgata tttgtgatta aatggaagta tgtatgcatg ctatcatact
     8941 ataggtctgc aacaaagaat ttcaccggat aaaaatttca ttcaccgaaa accgtgaata
     9001 aaaaagtgct gaaaaaaaat attttgtgac agcatttatt ctcagtctta acatttgaat
     9061 ttttgcaatc aagtataagt aggtatttga acggttttgc gtgtcaaaac ctgtgacatc
     9121 agtgcattca cggtaattgg tgaatttgta tataaggtat tgcatgtaat tgtaatgcga
     9181 aataattttg ttttgtcagc agttgttttt ttcaaaagaa gatcattctc ctgcacaaat
     9241 aatttctttt gtaatcccat aaattaaaca cactgattgc tttaagaaaa gaaggaaaac
     9301 gccgaaaatc ctaaatagag taggcatcac ttaaaaacct ttaaaaatgg caacagtaac
     9361 attcaaaaaa attgtttatc tccacagtgg aacaggtgta cgtcaccatc attattcatc
     9421 ttccgagggc atcatttccg gtcctgttaa cagcaattat cccggaacta ctgatacaac
     9481 ctccggtaca tacagtgccg atggtgctgc gcaggaaatt tacaatggtc aactggtgaa
     9541 tttcgctttt atggctgtga aagggtctgc cgatggcaat cagttattta ccagtgcagg
     9601 ggatcaaagc attcgcgtag gcagcaacaa tgtcactgtt ctggtagtgt atgcgcctcc
     9661 gggtggaatt ggtgtaaatg gtggtcccgg tgtatgggta gatgcattta atgttgatac
     9721 ctgtgcattt agcgacgatc tgcatttcat tcaggttttc acacctccaa caccaccgga
     9781 taatctcgac gcggcaaaaa cagattttgc caacatggaa ggcgaaattt ctaccgtaac
     9841 agcagaacat atgcgcgcat ccactactgt tgatggtgca attccattcc ttgaatggaa
     9901 acaagtgaca tcaaatgatt cacctgttgg ttcgagagat gttgatttgg cacagggaca
     9961 atcgggacag atttggtttg ctttctataa aacaccacct gctcctccgg caccttcaat
    10021 tccacacttt aatgatcgtt taggttggat ctatttgtct ccgggagtac ttgttggcgg
    10081 tggtggattt ggaatcggac ctgatggtaa accgcatccg attgatcctg atggaccatt
    10141 gatgcaacaa ttactcagca catttgctat gatctcggtg tcggcaaata tgcatgaaaa
    10201 attgcaaatg gaggccagaa gaatcgcttc cgataattta agaatgattg cagattcagt
    10261 acagaataaa agactgaaat agtaattatc aacccaaaac cccggagtga aaacttcggg
    10321 gttttatttt tttgcgcatt gaaattattc tgtcattagt ctttgtaaca aggtaatagc
    10381 agcatcagct atcaccgttc ccggaccgaa aattccggca acaccggctt cttccagaaa
    10441 ggcgtagtct tgttgtggaa taacacctcc aacaactaca agcatatctt cgcgtccttg
    10501 ttcttttaaa agatcaataa cacgtggtac taatgtttta tgtcctgcag ctaatgatga
    10561 aatacctatg atatgcacat cattttctat ggcctgttta acggcctctt cgggtgttaa
    10621 aaataatggt gatatatcta catcaaatcc aatatcagca aatgcagtag caattacttt
    10681 agcacctctg tcgtgaccat cttgtccgag ttttgcaatc agtatacgtg gacgtctacc
    10741 ggcttttccg gcaaatgcat cgcttagatt gcgcgcttgt ttaaaggttt cgttctgcat
    10801 aacttctttt tgatacacgc cggcaatggt gcgggtttcg gcagtatagc gtccgaaggc
    10861 tgcttccatc gcatcactta tttcgcctaa agtagcgcga attcttgcag catctactgc
    10921 cagtgcaagt aaattgccgt tgcctgtttt tgcggcatct tcaatttttg caagtgcttg
    10981 tttcaccgca ttgttatcgc gcgagttgcg cacttcatgt aatctttcaa tttgcgcatt
    11041 tcttaccgcc gaattatcta cttcaagaat atcgatattg ttttcttctt ctgttttaaa
    11101 tgcatttaat ccaacaatca catctgttcc gctatcaatg cgtccttgtt ttttggcagc
    11161 agcttcttca atgcgcattt ttggaattcc tgcttcaatg gctttctgca tgccgccgag
    11221 gttttcaact tcctgaatca gcttccatgc atcgtgaatc aatttatctg tgagtgcttc
    11281 aacaatatta ctcccacccc agggatcaac agtatcacaa atacctgttt ccttctgcaa
    11341 ataaatttgt gtatttcttg caatgcgtgc agaaaaatcg gtgggtaatg caatggcctc
    11401 atccaaacta ttggtatgca aactttgtgt acctcccaag gctgcagcta aggcttcaat
    11461 gcatgttcgt gctacattat taaatggttg ttgttcggta aggctccatc ctgatgtttg
    11521 acaatgtgta cgcagcatca gtgatttatt gtttttcggt gcaaattgct ccatcagcct
    11581 acaccatagt acacgcgctg ctctcagctt cgcaacttcc ataaaatggt ccatgccgat
    11641 gccaaagaaa aaagaaagtc gcggtgcgaa atcatcaact tgcatacctg ctgctattcc
    11701 tgtgcgcaca tattcaacac catctgcaag ggtatatgcc aattcaattt ccggcgtggc
    11761 acctgcttcc tgcatgtgat aaccggaaat ggaaatggaa ttaaatttcg gcatattttt
    11821 tgcgcaataa tcaaaaatat ctgccactat gcgcatgctt gaaacaggag ggtagatgta
    11881 agtattgcgc accatgaatt ctttcagaat atcattttgt atagtgcctg ccaattgttg
    11941 tggtgttaca ccttgctctt cagccgcaat aatataaaat gccatgatag gaattacagc
    12001 tccgttcatg gtcatgctta cactcatttg atcaagtggt atttgatcaa ataaaatttt
    12061 catatcctca accgtatcaa tagcaacacc tgccatgccg acatctccaa aaacacgtgg
    12121 attatcagaa tcatatccac gatgtgtggc aagatcaaat gctacactca atcccttttg
    12181 tcctgcagca agatttttac gataaaaagc attggatgct tcggcggttg aaaatcctgc
    12241 atattgcctg atggtccacg gctgcattac atacatggtg ctgtatgggc ctcgcaaaaa
    12301 tggtgcagat ccgggaagag atttttttac gacatcaaaa cctgcatccg gttgaacttg
    12361 taccgatggc aattgataat gatgataagg aatattgcgc aatgatcgtt tactcatgtt
    12421 caggcattgt gtgtttcaat aaaaattgac catgcctttt ctccaaattg gcgagtcaat
    12481 gattcgatat agtagctgcc gtttgctgca tcgcgatagc gatccatata actttcgtac
    12541 cgtaataaat tctgaatgtt tatagccagg cgaacacttt gtgcatcctg ttgtaaatcg
    12601 aaagtaggaa tcgatagtgc gtcaacacct ccgagcaatg cactcatggc tgctgtggtg
    12661 ctgcgcaata aattgctgtg tttgtcttct tgggtaaatg aatgggttga aacaacggca
    12721 tgtatgtatg gtgtgggaat aaaatccaat tcaggatcat aagctttcac cagattattc
    12781 cataagattt tgaatgcacg caatttcgcc atttcgaaga aataatttgt gccaacacca
    12841 aatcggaagg tgaacagttg tgttatttca aataatgaaa gattgcgttt ctccatttct
    12901 tcaaaataaa cagcagtgcg atgtaatgct ttcactaatt gatcaatcga tgaagcaccg
    12961 gcatctgcaa ggtctgtggt atctacttta attaatcggc agtcttgcag gtggccgtta
    13021 attctcaaaa ttgcatcagc aagcctgtcg aattgcgcat tggtagaagc gggatatcca
    13081 ttcttttccc aatatgaaat cggatcgaat tgtatcgatt gaatcagtct gtcgcgatca
    13141 ccaagaaaac cgatgcgttc gcgccagctt ccggcagtgt gttcgttgat cggacctgtt
    13201 gcatcaaaat gtaaattcgc aggtgatccc atttttctga tgccgtcaaa aatagcatcg
    13261 tagtcgctat caacttcagc atgtaatcgc agatcaataa caccacgctg acgggcttgc
    13321 tgcacaagct catgcatatt tgtcgaagcg gagacgtccc aatcctccgt aatattccag
    13381 gtgagatgtt gttgatgttg attgccgact tctgcggcaa agtcgtcatc catatccgca
    13441 aggaaatcag cattggcatc aggctcaaat tcgggaaggt ctgatgctgt ataaagaggc
    13501 ataatctcga taccttcctt tatatgaacc agcttttccc gaaagtcggc acctttcagg
    13561 tcttttgtca cctgttgcag ccatggagcc ttttcaatat cattgtttat aagctcgtcc
    13621 atcttgatgt attgggttgc aaagatacgg actattgcac ttgtgtatca tgtcattatc
    13681 tgatcgaaag tcctatcttt atagatacaa acccccgatg aaaactgttt atcgtaaact
    13741 cgaagcctat tctgctgctg caattgcact aatggctgtt aaaacggcag aagcacaagt
    13801 gcattatgct gatgtagatc ctgatatcgt gcttgattca cccgatgcat tcgctttgat
    13861 tgacctcaat ggcgatggtc ttgctgatat taaattcgct aatcagtctt tttatttgtt
    13921 gctgtggact ttctatagtg aagaaaagcc atacctgttt caatatatca atgcctcggc
    13981 ttatgctgaa ctatttgcct ataatgaaaa ctcggtagct tataccggta gtgaaggtgg
    14041 actttcaggt ggtgtatggc atgtagcaaa ggctatagag gaaggcgagg tggttaatgc
    14101 tgaattagat tttgaggaat ggggcgtgcc catggctgaa aaaatttata gttgtacgct
    14161 taccgcagga tttggagcta cttcctgcgt gttctccgat tctgttggaa agtggcaacc
    14221 tgaattggca aatcgtttta ttggattgca tttcactaat attaccgata taggatattt
    14281 cggttggata cgatgttcta tcgccaacga cggacgaaaa cttaccattc acgattacgc
    14341 ctacaatgcc actcccgaac aggcaataat tgcaggcgat acacttgctg ttggtatttc
    14401 tgaggcaact ttacagggaa ctcaaatgta tactgacggc aataatgttt tgcatgcaaa
    14461 aacgacgtca tcgggaaaca cccgtttgca gctttttgat atcagcggtc gtgagattgt
    14521 attcttgcaa aataatattg gcgaatttaa ttatgatctc actgctttac ctgcgggacc
    14581 atatattgtt cgtttaacac aaggtgcatc ctcctcaacg attaaaatta ttcgtaaatg
    14641 aacgcatgga ttaaaccggc aatattaaca acgtgtttca tttgcagcaa taaattgcag
    14701 gcgcagtggt caacggttga ctcaacattt agtgatgacg ggattgcaat tatttcagca
    14761 ggtgccggga atgacagatg ctatacttcc gcggtgcaaa gtgatggcaa actcattatt
    14821 gttggaaata gcatctatga aagcggcggt tatttttttt tcatatcgag attattttcc
    14881 gacggcaccc c
//
LOCUS       DAOQOU010000067        14804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2065_length_14804_cov_5.329649, whole genome shotgun sequence.
ACCESSION   DAOQOU010000067 DAOQOU010000000
VERSION     DAOQOU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14804
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_14804_cov_5.329649"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLM90_07420"
     CDS             <1..764
                     /locus_tag="PLM90_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMZ89238.1"
                     /translation="LKHEPYSNQCFILKNGSSSVLASYNSDDKVFQRINKASAYGIKP
                     RNAEQVFALSMLLNPNVKLIALQGQSGTGKTLLALASALEQRRQYKQIFLARPIIPLN
                     NKDIGYLPGDIKSKLNPYMEPLWDNLKMIQNQFEQADSEYKAIAQMIEQEKLVIQPLA
                     YIRGRSISNIYFIIDEAQNLTPLEVKTIITRAGENTKIVFTGDINQIDTPYLDTHSNG
                     LSYLIDKIKNHPMCSVITLQRGERSELANLANDLL"
     gene            849..2735
                     /locus_tag="PLM90_07425"
     CDS             849..2735
                     /locus_tag="PLM90_07425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HMZ89239.1"
                     /translation="MANTNYNYSEEHIRTLDWIEHMRMRPGMYIGKLGDGSSPDDGIY
                     ILVKEVIDNAIDEYMMGYGKRIDVTIDKHQVTVRDFGRGIPLGKVIECVSVMNTGAKY
                     DTAAFQKSVGLNGVGTKAVNALSQYFRVEAYREGKTKVAEFEKGLIKKDSAISKTEQK
                     DGTYVTFIPDNTIFRNFHFIPEYLDNQLWNYVFLNSGLTINFNGQKYYSQNGLHDLLV
                     RNSNAETNRYPIIHLKGDDIEIALTHGNEYGEDYYSFVNGQNTTQGGTHLAAFREGLV
                     KTVREFYKKDFDASDIRQAIVGAIAIRVQEPVFESQTKTKLGSIYTSPDAQVTVKSFV
                     VDFVKKELDNYLHKNPATADALLKRILQSERERKDIAGIKKLANERAKKANLHNRKLR
                     DCRYHLGEDKHEKTLRSTLFITEGDSASGSITKARDVESQAVFSLKGKPLNVFGLTKK
                     IVYENEEFNLLQHALNIEDGIDGLRYNQIVVATDADVDGMHIRLLLLTFFLQFFPEVV
                     KNGHLYILQTPLFRVRNKKETIYCYNEEEKQVAVNKLGNKPEITRFKGLGEISPDEFS
                     GFIGENIRLEPVIIGKEQSIQSTLEYYMGKNTPDRQTFIIRNLRVEKDVIDEPAIVEA
                     ESEV"
     gene            complement(2732..4339)
                     /locus_tag="PLM90_07430"
     CDS             complement(2732..4339)
                     /locus_tag="PLM90_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89240.1"
                     /translation="MAHKKPQKPEDHPIDKLFRDSLENRDFPYDEKYWTEAEKTLADF
                     NAKAGKRRGGKWLFGGIAITIVATLITIFIVQSNKQQEETSQITKNTIQQQDLSKNID
                     DQTIQKEQNDKSNEVSKSVQQIKHKDAPVLRDEMRKKLREKRDHKLKEAKQEAKNNSD
                     NSRNKSVNQSNNPVNTQAGNIGNSSINPQNNANKQNVQPDNNDSHASTGLNQQDKSNT
                     EASGKAGGEEKTNVTDNSNQTTTNDQQEQHKTGEKGGNTEVNPDLNQPEQTTNGANEL
                     NNKVETPVNIQDSVNQNNISNGQNNPNKPSQFKTRKSTLGLQWHIGLQGGVYHTSPQF
                     KDVWNYSNVHIYPDPNTLFPAGEVSFGMMLPKVPFTLSSGIGLITMQDKSTYQINILN
                     IDTTYDYITDTSGLVTDSIPLLDSSYATYYNETPNKLTYVQIPLLIGYEWTRQSWHIG
                     IATGPVIGLKLGDSGIYETLVPTTYYDRVTWYWKAEPQVSYSFNEHWRLALQGNLQWQ
                     LSNTYLDSQIDLKFGLQGLQIGVQYDF"
     gene            complement(4346..4897)
                     /locus_tag="PLM90_07435"
     CDS             complement(4346..4897)
                     /locus_tag="PLM90_07435"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ89241.1"
                     /translation="MVIEPSLLVLSAKGDKKAQHALYKQAFSLLMGVCSRYATDRDEA
                     RSLLNQGFLKMLMHADQRKQNVPVEAWMRRVMINTIIDVYRQQKSYREHTKLVDYSDH
                     STVHAYEGSDDHRHMEKINADALFHMLTKLPEMTAKVFNLFAIDGYSHKEIAEMLRMS
                     EGTSKWHVNHARTLLKNMLNELD"
     gene            5397..8039
                     /locus_tag="PLM90_07440"
     CDS             5397..8039
                     /locus_tag="PLM90_07440"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HMZ89242.1"
                     /translation="MAKKKQETPKEQAQPIQPIPVKQGTTHVSHMYKDWFLDYASYVI
                     LERAVPDIRDGYKPVQRRILHAMREMDDGRFHKVANVIGQTMQYHPHGDAAIGEALVN
                     MGQKDLLIETQGNWGDVRTGDDAAAPRYIEARLSKFALEVVFNKETTDWQLSYDGRKS
                     EPIYLPVKFPMVLAMGVEGIAVGLSTKILPHNFCELILASIEVLKGNDTNILPDFATG
                     GMIDVSEYNHGERGGRIRCRAKIEVADKKTIVVKEIPFSTTTTSLIDSIIKANDKGKI
                     KIKKVTDNTAKDVEVAIELAPGISPDVTVDALYAFTDCEISISPSTCVIEDDKPLFLS
                     VDEILRRSTKNTRDLLKLELELKLQELNEKWHFSSLEKIFIENRIYRDIEECETWPDV
                     IKAIDKGLDPFKSKLMREVTEEDIVRLTEIKIKRISKFDAFKADEYIRDLEKEMKEVK
                     HNLRHLTDYAIKYFEELLRKYGKGRERKTEIRKFDTIEAKAVVANNAKLYVNKEEGFV
                     GYGLKKDEFLCDCSDIDDIIAFTEDGKMKVVRIADKVFIGKKILYANVWKKEDERMTY
                     HMIYTDGKSGKTFAKRFNVTAITRDKEYDLTQGNAKSKVLYFSANPNSESEVVSIQLH
                     PSARAINKLFDFDFGTLAVKGRSSMGNTLSKYPMRKIIQKEKGTSTLGGRKLWVDETV
                     GRLNVDARGRYLGEFDTGDHILVIYRDGNYELTNFELSNRFEMREILLLEKYNEQKVV
                     SAAYWDNEQKNMFVKRFYIESVVPDKKYLFISEAPGSKLYVASTAKKPRIEIIVVKGR
                     KQEEVPEIIEVESFIDVKGWKSLGKRLSSYEIRKVTGLPSDEPVEETPPPAKEGSVKV
                     GSQLEWDLDNDDKSGKDGKQTKLF"
     gene            8177..8758
                     /locus_tag="PLM90_07445"
     CDS             8177..8758
                     /locus_tag="PLM90_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89243.1"
                     /translation="MPAVKNDIFSKFMVPFMQWYLTMIAVTVVIDYILHRVGWVHMGI
                     YLGYLGTALVISSFIYSLRKRKIIQSGTPKKLLQWHEYGAWIGTIFILVHAGIHFNAI
                     LPWLAVLMMLLTVASGLVGKYILKKATESFRIKKQELLDAGKTPAEIDQVLLFDAVTV
                     EAMKKWRVVHMPITYFLAIFSIIHIITAIMYGA"
     gene            8755..9642
                     /locus_tag="PLM90_07450"
     CDS             8755..9642
                     /locus_tag="PLM90_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMZ89244.1"
                     /translation="MNKIVILMTALFVVVLVIKFPHRMINPGPLQEAHAALDNDCKNC
                     HQPFWGIPNANCISCHNPADIGRDTTGVDTGKILFHEALTNRSCIGCHDDHKGRIPLV
                     AINSFDHKMPGTAKGLNCVQCHTAPEDKLHPIVSKNCASCHDTKNWSSISAFDHAMIN
                     KVELQQCGTCHQAPQDQMHIAVTSTSCSNCHQTGAWSPATFNHDMYFVLDGNHNVRCN
                     TCHTKQDFKTYTCYGCHEHTERKMVAEHSEEGINNINDCARCHGSANEHDVKRSVGDG
                     NSGEHRKRKEGEHEEDDDD"
     gene            9689..9991
                     /locus_tag="PLM90_07455"
     CDS             9689..9991
                     /locus_tag="PLM90_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89245.1"
                     /translation="MKLFIATMLVIGTITGEKKSASFVLRNESASSIPLVIPGVMNPN
                     LSPMSNSKVTLEEGQEIFFLEKKKRYLLLEVSPELNGDTLIVNDLIKKRKQELDLN"
     gene            9995..10687
                     /locus_tag="PLM90_07460"
     CDS             9995..10687
                     /locus_tag="PLM90_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ89246.1"
                     /translation="MAHYLIIGASSGIGREVVNQLISSGHQVTATYNNHNADLDSAVT
                     WHHFNAIDAEPIINWMPDHLDGVVYCPGSIQLKPFHRIHAQQFADDYQLQVSGMIRIL
                     QAAYPALKKSSHAAVVMYSTVAVQRGFTFHTLVSASKGAIEGFARALAAEWAPSIRVN
                     VIAPSITDTPLAEGLLNTAEKIDANAQRHPLKRIGTTQDIAKMTTFLLSPDSAWITGE
                     ILHVDGGMHSIK"
     gene            10693..11154
                     /locus_tag="PLM90_07465"
     CDS             10693..11154
                     /locus_tag="PLM90_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ89247.1"
                     /translation="MGVYTIYEEQFLPTSLEDAWKFVSDPHNLKVITPDYMRFNVLTK
                     DLPDSVYSGLIIQYYVSPLLRIPMKWVTEITFVEPLKYFVDEQRVGPYRIWHHEHHLK
                     AVDGGVLMIDRVTYQPPLGFLGSIANALFIAKQLKGIFVYRRKKLEQLFPQ"
     gene            complement(11157..14108)
                     /locus_tag="PLM90_07470"
     CDS             complement(11157..14108)
                     /locus_tag="PLM90_07470"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HMZ89248.1"
                     /translation="MQKVKYPISTLTLHTLALLILLVPQCTDMMAQVVEPGSKYASPV
                     HFIPNNGQLTDFNGDFVPNVYAYLQSGDVQVFVTSKGLTYMQYGEVKPDLSEGKKKVR
                     DWERIDMQLDGAKINAGNILFDEIGSEKMQYYVSGYDDPIVVQGCNRILIQEIYPGID
                     WELLVNEAGFKYNFIVHAHADLSQIRLHYITPLPISSPDGDIRIHAKQGDIIERAPYS
                     FLHATNEAVSSIILSEGKSEMGEIFLFQTGDYDHNEELIIDPSLWWCTYYGGVFNEIF
                     NCVDADLSGNIIAVGWTESANFPTYNAGTYYQGTKSVNEDVVIVKFSDIGERLWATYY
                     GGNAEDFGYGVSTDASGNIFVTGETVSSNFPVYNAGTYFQAGLNGDEDVFIIKFNAAG
                     TRQWATYYGGDNKEGGYSIATDNTGNIFVTGYTKSSTFPKLNAGTYYQASMAGVQDAF
                     MLKFSNTGTRLWATFYGGNAFDEGNALATDSDGDVFMTGTTKSTTLPIYDAGTYFQGT
                     MAGDTDAYVIKFSNTGSRLWASYFGGTDAEYGMSVVCDQNDNVIFGGYTQSSVLLTHV
                     PDLSWYWQIMNAGGTDMYIFETDNAGNSIWTTFFGGSGNEKMDTKDALDISGCNDLYI
                     SGTSFSYDLPLYDAGCSSYYDGTNAGEGDIWLTRFTDAGQITWSTLFGFENEDIASCL
                     ATSPLDGSSLFMTGVYHEYDPGEAVPLVNPGGSTYYDGSHNGDDECFVSKFIAVPLNL
                     DLSYSNLCYCLDSAIVTPSCGVAPYTYLWADGQTDSIATGLCPGLWDVEVTDADCNVE
                     TVTFLIDCILPLELINFTGYQDKGRNQLQWEITNDPVSEFFNLYRHFNGGDNLITSIP
                     VTNITDYHFSDDIFSEGIMYYTLEGISSTGEVIFSETIAVEETSEKADLIITYPEYER
                     AKLILDAADNESTYVDIVDMRGVVMMRFPWNLSPGRNYTYFEHPDLPKGYYLVTVSGG
                     TTDQHVGFYHQ"
     gene            complement(14258..>14804)
                     /locus_tag="PLM90_07475"
     CDS             complement(14258..>14804)
                     /locus_tag="PLM90_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ89249.1"
                     /translation="AGKWENEIRALRKTILSCQLQEELKWGTPCYTLDGRNIVLIHIF
                     KEYCAVLFFKGVALQDAQKLLIQQTENVQTARQMRFTSLSEIKKTTTALKAYIFEAIE
                     VEKSGVKMPLKKTKEFSMAEEFKSALNKSTKLKKAFEALTPGRQRAYLLHFSSAKQSA
                     TRIARIEKYVPKILAGKGIDD"
BASE COUNT         4396 a         2935 c         3107 g         4366 t
ORIGIN      
        1 ttttaaaaca tgaaccttat tccaatcagt gctttattct gaagaatggc agcagcagtg
       61 tgcttgcgag ttataattcc gacgataagg tttttcaacg tatcaataaa gcttcagcat
      121 atggcattaa accgcgcaat gctgagcagg tatttgcgct gagtatgttg ctcaatccga
      181 atgttaaact tattgcctta caaggacaaa gtggaacggg aaaaaccttg ttggcattgg
      241 ccagtgcttt ggagcagcgc agacaataca agcaaatatt tcttgccagg ccgattattc
      301 cattgaataa taaagacatc ggatatttac cgggtgatat taaatcgaag ttgaatccat
      361 atatggagcc actttgggat aatctgaaaa tgatccaaaa tcagtttgag caggccgata
      421 gtgagtataa agcaattgcg caaatgatag agcaggaaaa attggtgata caaccgcttg
      481 cctatatacg aggtcgcagt atttcaaaca tttattttat cattgatgaa gcgcagaacc
      541 tcacacccct ggaagtgaaa actattatca caagggcagg ggagaataca aagattgtct
      601 ttaccggcga tatcaatcag atagatacac catatctcga tacgcatagt aatggattga
      661 gttatctaat cgataaaata aaaaatcatc cgatgtgttc tgtgatcaca ctccaacgcg
      721 gagagcggtc tgaactggcg aatctggcaa acgacctgtt gtaagtcacg acagttatgc
      781 acaggaaaat ggggagcaca tttcaacgat tgacctaata ttgtgtaaga aaggaaaact
      841 gaagcggcat ggctaatacg aactataatt attcggaaga acatatcaga acgctcgact
      901 ggatcgagca tatgcgcatg cgtcccggta tgtatatcgg gaaactgggc gatggttcat
      961 ctcctgatga tggtatttac attcttgtaa aagaggtgat cgataatgct attgatgagt
     1021 atatgatggg ttatggaaag cgcattgatg taactatcga caagcatcag gttaccgttc
     1081 gcgattttgg tcgtggtatc ccactgggaa aagtgataga atgcgtgagc gtaatgaata
     1141 ccggtgccaa atatgacaca gcagcattcc agaaatctgt tggtttgaac ggtgtaggta
     1201 ccaaagcggt gaatgcactt agtcagtatt ttcgtgttga agcatatcgc gaaggcaaaa
     1261 ccaaagttgc tgaattcgag aaaggcttga tcaaaaaaga cagcgccatt tctaaaacag
     1321 aacaaaagga tggcacctat gtgaccttta taccggataa caccatcttc cgcaattttc
     1381 attttattcc cgaatacctg gataatcaat tgtggaatta tgtattcctg aattccggac
     1441 tcaccatcaa tttcaatggt cagaaatact attcacagaa tggtttgcat gatctgttag
     1501 tgcgcaatag caatgccgaa acaaatcgct accccattat acatcttaag ggcgatgata
     1561 ttgaaattgc acttacacac ggcaatgaat atggagaaga ttattattca ttcgtaaacg
     1621 gacaaaatac cacacagggt ggaacgcatt tggcggcgtt tcgcgaaggc ttggtgaaga
     1681 ccgtgcgcga attctataag aaagattttg atgcatccga catccgtcag gctattgtgg
     1741 gcgctattgc catcagggtg caagagccgg tgtttgaatc acagacaaag accaaactcg
     1801 gttcgatata tacctctcct gatgcacagg taactgtgaa atcatttgtt gtggattttg
     1861 tcaagaaaga gcttgataat tatctccata aaaatcctgc tactgccgat gccttgttaa
     1921 aacgcattct tcaaagcgaa cgtgaacgca aagatattgc aggtatcaag aaattggcaa
     1981 atgagcgcgc gaagaaggca aacttgcata acaggaaatt gagagattgc cgatatcatc
     2041 ttggagaaga taaacatgag aaaacgctac gcagtacttt atttatcact gagggtgata
     2101 gtgccagtgg aagtatcacc aaagcgcgcg atgttgaatc gcaagcagta ttcagcttga
     2161 aaggtaagcc actgaatgtt ttcggactga cgaaaaaaat tgtttatgaa aatgaggagt
     2221 ttaatctcct tcagcatgca ttaaatattg aggatgggat tgacggtttg cgctataatc
     2281 aaatcgtagt tgcaaccgat gccgatgtgg atggcatgca tattcgattg ctattactga
     2341 ctttcttttt gcagtttttc cctgaagtag tgaaaaatgg tcatctctat attttgcaaa
     2401 cgccattatt ccgtgtgcga aataaaaaag agacaattta ttgctataat gaagaagaga
     2461 aacaggttgc agtcaataaa ttgggaaata aacctgagat cacacgattc aaaggacttg
     2521 gagaaatatc tccggatgaa ttttcaggct tcattggtga aaatattcgc cttgaaccgg
     2581 taattatcgg taaagaacaa tcgatccagt ctacacttga atattacatg ggaaaaaaca
     2641 cccctgatcg ccaaacattt atcattcgca atttacgcgt tgagaaggat gtgatcgatg
     2701 agcctgcgat cgttgaggct gaatcagaag tttaaaaatc gtactgcaca ccaatttgta
     2761 atccttgcag accgaatttg agatcaattt gggaatccaa ataagtattg gaaagttgcc
     2821 attgtaaatt tccttgcaat gccaaacgcc aatgttcatt aaaactgtat gacacttgcg
     2881 gctcggcctt ccaataccag gtaacacgat cataatatgt tgttggtacc aatgtttcat
     2941 atattccact atctcccagt tttaatccta ttacaggacc cgtggcaatg ccaatatgcc
     3001 aactttgcct cgtccattca tatccaataa gcagcggaat ctgcacatag gttaacttat
     3061 ttggcgtttc attataataa gttgcataac tgctatctaa caacggaatg ctatcggtca
     3121 ccaacccgga agtatctgta atataatcgt atgtggtgtc tatgtttaga atattaattt
     3181 gatatgttga tttatcttgc attgtaatta aaccaatacc tgagctcaag gtaaatggca
     3241 ccttaggcaa catcatacca aatgatactt caccggcagg gaatagggtg tttggatcag
     3301 ggtagatatg cacgttgctg taattccaaa catctttaaa ttgagggctt gtatggtaaa
     3361 ctccgccctg caatccaata tgccattgta atccaagggt tgattttctt gttttgaatt
     3421 gagaaggctt attaggatta ttctgaccat tagaaatatt gttttgattt acggaatcct
     3481 gaatgttaac aggcgtttcg actttattat tcaattcatt tgcgccattt gtagtttgtt
     3541 ccggttgatt caaatcaggg ttaacttcag tattaccacc tttttcacct gttttgtgct
     3601 gctcttgttg gtcgttggtt gttgtttgat tcgaattgtc tgtgacatta gttttttctt
     3661 cgccacctgc cttgcctgat gcttcggtgt tgcttttatc ttgctgattc aatccggttg
     3721 atgcatgtga gtcattgttg tcaggttgca cattttgttt atttgcattg ttctgtggat
     3781 tgatactgct attaccgata ttcccggctt gggtattaac gggattgttt gattgattga
     3841 cggatttatt tcttgaatta tcgctattat ttttcgcttc ttgcttcgct tcttttaatt
     3901 tatggtcgcg tttttcacga agttttttcc gcatctcatc tcgtaatacc ggtgcatcct
     3961 tgtgcttgat ttgttgaaca cttttgctta cttcgttgct tttgtcgttt tgttcttttt
     4021 ggatagtctg atcgtcaata tttttagata ggtcttgctg ttggattgta tttttcgtga
     4081 tttgtgaagt ttcttcctgt tgcttgtttg attgcacaat gaaaatcgta atcaaggttg
     4141 ccacaattgt tatggcaatg ccaccaaata accatttccc gccgcgacgc tttcctgctt
     4201 tggcattgaa atcagcaagt gttttctctg cctctgtcca atatttttca tcatacggga
     4261 agtcgcgatt ctccaggctg tcgcgaaaca gcttgtcgat cgggtgatct tccggttttt
     4321 gtggtttttt gtgtgccata atgctttaat cgagttcgtt aagcatattt tttaataatg
     4381 tacgcgcatg gttgacatgc catttcgatg tgccttcgct catcctcagc atctctgcaa
     4441 tttctttgtg actatatccg tcaatcgcaa acaggttgaa tactttcgca gtcatttcag
     4501 gcagttttgt aagcatatgg aataatgcat cagcattgat tttttccata tgccgatgat
     4561 catcacttcc ttcataagca tgaaccgttg aatggtcgga gtaatcaacc agtttcgtgt
     4621 gttcacgata tgacttttgc tgcctgtaga catcgataat tgtattaatc atgactctcc
     4681 gcatccaggc ttcaaccgga acattttgct ttcgctgatc tgcatgcata agcatcttta
     4741 gaaaaccttg gttaagtaag ctcctcgcct catcacgatc ggttgcatac ctgctgcaaa
     4801 cacccataag caagctgaat gcctgcttat ataaagcgtg ctgggctttt ttatcgccct
     4861 ttgcagataa taccaatagc gatggttcta tgaccattgt tttccttgtt ccttttgtgc
     4921 taataaaatt aggtacagta tataaactgt gttgaagcat aagcgattac atcatactat
     4981 caacctttca tgctcccatg caaaattaga aaaaccacca tcccgaacgc atccgggcgg
     5041 tggtattttg actagcacta aggaacacag tgaacgaaaa ctgctcttgc ccgggcataa
     5101 caaagccaca tctgacagaa ctttcagggt attggaagat gtagtttgcc tcatgttcgg
     5161 gtacggattt taccccgtta tggcttatca cgcattcggc tttgaaaagg ttgggttggg
     5221 cgtaagcttt tttttacgac ttttcgcaac acgaaaagca acatttgcga tttttgcaac
     5281 tccccgaagt catttgcaca ataattttgt atttgtccta atgcccatca aatcgtggaa
     5341 aactcgcttc ataagccata ggcattgcag tatttttgac cctcaccgcg gcagttatgg
     5401 ctaagaagaa gcaagagaca cctaaggaac aggctcaacc tattcaaccg attccggtaa
     5461 aacaggggac tacccatgtt tcacatatgt ataaggattg gtttctcgac tatgccagct
     5521 acgtgatcct ggagcgcgca gtacctgata tccgagatgg atataaaccg gtgcaacgtc
     5581 gcattttaca tgccatgcgt gaaatggacg acggtcgttt ccataaagtt gccaatgtaa
     5641 ttggtcaaac catgcaatat caccctcatg gtgatgctgc tatcggcgaa gccttggtga
     5701 acatgggaca aaaagacctc ctcattgaaa cgcaaggaaa ctggggtgat gtaagaaccg
     5761 gcgatgatgc tgctgctccc cgatatattg aagcacgact ttcaaagttc gctctggagg
     5821 ttgtatttaa taaagaaact accgattggc agctgagtta cgatggtcgt aaatctgaac
     5881 ctatttatct gccggttaag tttcctatgg tacttgccat gggcgttgaa ggtatcgccg
     5941 ttggtttgtc gaccaagata ttaccgcata atttctgtga attaatactg gctagcatag
     6001 aagtgttaaa aggaaacgat actaatattc ttcctgattt tgcaacagga ggaatgattg
     6061 atgtttcgga atacaaccat ggcgagcgtg gtggaaggat tcgatgcagg gcgaaaattg
     6121 aagttgctga taaaaagaca attgttgtga aagaaattcc tttcagcaca acaactactt
     6181 cgctgattga ctccatcatc aaagccaatg ataaaggcaa gataaaaatc aaaaaggtta
     6241 ccgataatac tgcaaaagat gtagaagtag ccatcgaact tgcaccggga atttcacctg
     6301 atgtgacggt ggatgcttta tatgctttta ccgattgcga gatttcaatt tcaccatcaa
     6361 cctgtgtgat cgaagatgat aagcctttgt ttttgtcggt tgatgaaata ctgcgcagaa
     6421 gtaccaaaaa tacccgcgac ctgctgaaac tggagctgga attaaagctg caggaactca
     6481 atgagaagtg gcatttcagc tcgcttgaga aaatattcat agaaaatcgc atctatcgcg
     6541 acattgaaga atgtgaaacc tggcctgatg taattaaagc tatagataaa ggcctcgatc
     6601 cattcaaatc taaactgatg cgcgaggtta cggaagagga tattgttcgc cttacagaga
     6661 tcaaaatcaa acgcatcagt aagttcgatg catttaaggc tgatgaatat attcgcgatc
     6721 ttgaaaagga aatgaaggag gtgaagcata atttacgtca cctgacagat tatgccatca
     6781 aatattttga agaactgctt cgtaaatatg gcaaaggccg tgaacggaaa acagaaattc
     6841 gcaaattcga taccattgag gcaaaagcag tagttgcaaa taatgcaaaa ttgtatgtaa
     6901 acaaggagga aggtttcgtt ggatatggat tgaagaaaga tgaatttctc tgcgactgct
     6961 ccgatattga tgatatcatt gcatttaccg aagatggaaa gatgaaggtt gtgcgtattg
     7021 ccgacaaagt attcattggt aaaaaaatcc tctatgccaa tgtttggaaa aaggaagacg
     7081 aacgcatgac ctatcatatg atttatacgg atgggaagtc gggcaaaact tttgccaagc
     7141 ggtttaatgt aacggctatt acgagggata aagaatatga tctcacacaa ggtaatgcaa
     7201 aaagcaaagt gctttatttt tcggcaaatc caaatagcga aagtgaggtg gtgagcatac
     7261 aactgcatcc gagtgcacgt gctatcaata aattattcga cttcgatttc ggaactcttg
     7321 ctgttaaagg aaggagcagt atgggaaata cgctttcgaa gtatccgatg cgtaagatca
     7381 tacagaaaga aaaagggaca tcaacacttg gtggaagaaa gctgtgggtg gacgaaactg
     7441 ttggcagatt aaatgtagat gcgcgcggaa ggtatctcgg cgaattcgat accggcgatc
     7501 atattttagt gatttatcgc gatggtaatt acgaactcac caattttgag ctcagcaatc
     7561 gcttcgaaat gcgtgagata ttattgctag agaaatataa tgaacaaaaa gtagtatccg
     7621 cagcatattg ggataatgag caaaagaata tgttcgtgaa gcgattctat atagaatctg
     7681 tcgttcccga taaaaaatac ctcttcatct ccgaagctcc cggttcaaaa ctgtatgttg
     7741 cctctactgc taagaaacca cgtatagaaa ttattgttgt aaaaggccgt aagcaagaag
     7801 aagttcctga gatcattgaa gtggaaagct tcatcgatgt taagggatgg aaaagtttag
     7861 gcaagcgatt gagttcctac gagatacgaa aagttacagg tcttccatca gatgaacctg
     7921 ttgaggaaac tccaccacct gctaaggaag gctctgtaaa agtgggtagc caactcgagt
     7981 gggatctcga caacgatgac aaatccggta aagacggcaa acaaacgaaa ttgttttgag
     8041 ccggcaaacg tagaatttgt ctaagtaatt tatttttcaa aataagtgta aactgacgaa
     8101 tatcaccgct cggtaggata tcatgatgca attttggaat tgccattatc gatgaattgc
     8161 gataatgtgc aatttcatgc cggcagttaa aaatgacatt ttttcgaagt ttatggtgcc
     8221 atttatgcag tggtacctca cgatgattgc cgttacagtg gtgattgatt atattctgca
     8281 tcgtgttggt tgggtgcaca tgggtattta tctcggatac ctcggaacgg cactagtaat
     8341 tagttcgttt atttatagtc ttcgcaagcg gaaaattatt caatccggaa cacccaaaaa
     8401 gttgctgcaa tggcatgaat atggtgcatg gattggaacc atattcattc tggttcatgc
     8461 aggtatacat tttaatgcga tacttccatg gctggctgtt ttgatgatgc tactcactgt
     8521 ggcaagcggt ttggtaggta aatatattct caaaaaagca accgagtcat tcagaattaa
     8581 aaaacaggaa ttgctcgatg ccggaaaaac cccggctgag atcgatcagg tattattgtt
     8641 tgatgcagta actgttgaag caatgaaaaa gtggcgtgtg gtacatatgc ctatcaccta
     8701 ttttttggcc atattttcta tcatccatat catcactgca ataatgtacg gcgcatgaac
     8761 aagatcgtga ttttgatgac tgcccttttc gtggttgtac tggtaataaa attccctcat
     8821 cgcatgatca accccggtcc gcttcaagag gcacatgcag cattagataa cgattgcaaa
     8881 aactgtcacc aaccattttg ggggattcca aatgcaaatt gtataagctg tcataatcct
     8941 gcagatatcg gtcgtgatac tacaggtgtt gataccggga aaatattatt tcacgaagcg
     9001 ttgacaaata gatcatgtat agggtgtcat gatgatcaca agggaaggat tccgctggtc
     9061 gctatcaatt cattcgatca taagatgccg ggaactgcga aaggtttgaa ttgtgtccag
     9121 tgtcatacag ctcctgaaga taagcttcat cctattgttt ctaaaaattg cgctagttgt
     9181 catgatacaa aaaattggtc atcaataagc gcatttgacc atgcaatgat taataaggta
     9241 gagctgcagc agtgtggtac atgccatcag gcgccccaag accaaatgca tatcgcagtc
     9301 acttcaacat catgttcaaa ttgccatcaa accggagcat ggtcaccggc aacatttaac
     9361 catgatatgt attttgtgct tgatggtaat cataatgtac gatgcaacac ctgtcacaca
     9421 aagcaagatt tcaagacgta tacctgttat ggatgtcatg agcatacgga aagaaaaatg
     9481 gtggctgagc attccgagga aggcataaat aatatcaatg attgcgcacg atgccatggc
     9541 agtgccaacg agcatgatgt aaaacgaagt gtcggagatg gcaattcggg cgaacatcgc
     9601 aaacggaagg agggagaaca tgaggaagat gatgatgatt gaactcgctg agctttgaac
     9661 aatttcattg tacatccgtt tgccatgcat gaaactattc atcgctacaa tgctagtgat
     9721 cggtacgatc acaggagaaa agaaaagtgc aagctttgtg ctgcgcaatg aatctgcctc
     9781 ctccattcca ctcgttatac ccggagtgat gaatccaaat ctatccccaa tgtcgaacag
     9841 caaagttacc ttggaggaag ggcaagaaat attttttctg gagaagaaaa aacgttacct
     9901 actcttggaa gtaagcccgg agctaaatgg tgataccctt attgtcaatg atttgattaa
     9961 aaagcgcaag caggaattgg atttaaatta aattatggca cactatctca tcattggcgc
    10021 atcatcaggc attggcaggg aagtggtaaa tcaattaatc agttccggac atcaggtaac
    10081 agcaacctat aacaaccata atgctgattt agatagtgcg gtaacctggc atcattttaa
    10141 tgccatcgat gctgaaccta tcatcaattg gatgccagat catcttgatg gcgtggttta
    10201 ttgccccggg agcatacaac tcaaaccatt tcatcgcatt catgcgcagc aatttgccga
    10261 cgattatcaa ttgcaggtaa gtggtatgat tcgcatatta caggctgcat atcctgcatt
    10321 gaaaaagtca tcacatgcgg cagtagtaat gtattcaacc gttgcagttc agcgtggatt
    10381 cacttttcat accttggtat ctgcatctaa aggagccatt gaagggtttg ccagagcgct
    10441 tgctgccgaa tgggctccct caattagagt aaatgttatt gcgccttcta tcacagatac
    10501 accgctggca gaaggattac tcaatacggc tgaaaaaatt gatgcgaatg cgcaacgtca
    10561 tcccctcaaa cgcattggca ctactcagga tatcgcaaaa atgactactt ttctgctctc
    10621 gccggatagc gcatggataa ccggagaaat attgcatgtt gacggaggca tgcatagcat
    10681 taaataatta ttatgggagt ttatacaata tacgaagaac aatttttgcc tactagtctc
    10741 gaagatgcct ggaagtttgt ttcagatccg cataatctta aagtgattac tcctgattac
    10801 atgcgtttta atgtgcttac caaggacctt ccggatagtg tttattccgg attgattatt
    10861 caatattatg tttcaccatt attgcggata ccaatgaaat gggtaaccga aataacattt
    10921 gttgagccat tgaaatattt tgtagatgag cagcgcgttg gcccataccg gatctggcat
    10981 cacgaacatc atttaaaagc tgttgacggt ggtgtgttaa tgatagatcg cgtaacctat
    11041 caaccaccac ttggttttct cggttctatt gcaaatgcgc tgtttattgc taaacaattg
    11101 aaaggtattt tcgtctaccg caggaaaaaa ttagagcagt tgttcccgca ataactttat
    11161 tgatgataga accctacatg ctgatcggta gttccaccac ttacagtcac taagtaatat
    11221 cctttcggca aatcaggatg ttcaaagtag gtgtaatttc tgcccggact taaattccac
    11281 ggaaaacgca tcataacaac gccccgcatg tcaacaatat ctacataagt agattcattg
    11341 tctgctgcat cgagaattag ttttgcacgt tcgtattcgg ggtaagtgat aatcaaatct
    11401 gctttctctg aggtttcttc tacagcaata gtttcggaga atattacttc gccggttgag
    11461 gatatgcctt ccaatgtgta atacataata ccttcgctga aaatatcgtc gctgaaatgg
    11521 taatctgtaa tattagtgac aggaatgctt gttattaagt tatcaccgcc attaaaatga
    11581 cgatacagat tgaaaaattc actgacagga tcatttgtaa tttcccattg aagctgattt
    11641 ctgcctttat cctgataacc tgtgaaattt attaattcaa gaggtagtat acagtcaatt
    11701 aaaaacgtta ctgtttccac attacaatcg gcatcagtaa cttctacatc ccataatccc
    11761 ggacataaac cggtagctat ggaatctgtt tgcccgtcag cccaaaggta ggtgtatgga
    11821 gccacaccgc aagaaggtgt aacaattgcg gaatcgaggc aatagcataa gtttgaatag
    11881 gagagatcaa gattaagtgg aacagcaata aattttgata caaaacattc atcatcgcca
    11941 ttatgtgatc catcgtaata ggttgagccg cccggattga caagaggaac tgcttcaccc
    12001 ggatcatatt catgatatac tccggtcata aacaatgagc tgccatcaag tggtgatgta
    12061 gcaagacatg aagcaatatc ttcgttttca aatccaaaaa gtgtcgacca ggtgatttgc
    12121 ccggcatcag taaatctggt aagccagata tctccctctc ctgcattggt gccatcgtaa
    12181 taactgctgc acccggcatc atataatggc aggtcgtatg aaaagctggt tcctgaaata
    12241 tacaggtcat tacatccact gatatctaat gcatctttag tatccatttt ctcattacct
    12301 gagccaccaa aaaatgttgt ccagatacta ttgccggcat tatctgtttc aaaaatatac
    12361 atatctgttc cgcccgcatt catgatttgc caataccatg agagatcagg aacatgtgta
    12421 agcaaaacag aagattgggt atatccgcca aaaatcacat tatcgttttg atcgcaaact
    12481 acactcattc catattctgc atcagtacct ccgaaataac tcgcccataa tcgacttccg
    12541 gtattactga attttattac ataagcatcg gtatctccgg ccatcgttcc ttgaaaatat
    12601 gttcctgcat cataaatcgg cagggtagtg gatttcgttg ttcctgtcat aaatacatcg
    12661 ccatcactgt cggtagccaa tgcattccct tcgtcaaatg cgtttccgcc gtaaaatgtt
    12721 gcccataatc tggtgcctgt gttagagaat ttcaacatga aggcatcctg cacacctgcc
    12781 atactcgcct ggtaataggt gccggcattt aattttggaa aggttgacga tttggtatat
    12841 cctgtaacga aaatatttcc ggtattgtct gttgcaatgc tatagccacc ttctttatta
    12901 tcgccaccat aataggttgc ccactgtctg gttcctgcag cattaaattt gatgataaag
    12961 acatcctcat caccatttaa tccggcttga aaataggtgc ctgcgttgta tacagggaaa
    13021 ttacttgaaa ccgtttcgcc cgttacaaaa atatttccgg atgcatcagt ggacacgcca
    13081 taaccgaaat cttctgcgtt gccaccataa tacgttgccc ataagcgctc tccgatatca
    13141 gaaaatttta caatcactac gtcttcatta actgattttg taccctggta ataagtacct
    13201 gcattatatg tcggaaagtt tgcagattct gtccatccta cggcaatgat atttcctgaa
    13261 agatcagcat caacacaatt aaaaatttca ttaaaaacac cgccataata agtgcaccac
    13321 cataaagaag ggtcaataat taattcctca ttgtgatcat aatctcctgt ttgaaaaagg
    13381 aatatttcac ccatctcaga ttttccttca cttaaaatta tgcttgatac tgcttcgttt
    13441 gtcgcatgca gaaaagagta gggggctcgc tctatgatat caccctgttt ggcgtgaatg
    13501 cgaatatcac catcgggtga agaaatgggt aggggcgtaa tgtaatgcaa gcgtatctgt
    13561 gataagtctg catgagcatg cacaataaag ttatatttaa acccggcctc gtttaccagc
    13621 aattcccaat ctatgccggg ataaatttcc tgaattaata ttctattaca tccttgaact
    13681 acaatcggat catcgtatcc tgaaacgtaa tactgcatct tttcacttcc gatttcatcg
    13741 aagagaatat tgccggcatt tattttggca ccatccaatt gcatatcaat ccgctcccaa
    13801 tcgcgaactt ttttctttcc ttctgaaaga tcaggcttta cctcaccata ctgcatgtag
    13861 gtaagccctt tcgatgtgac aaatacctgc acatcgccgg attgtaaata agcgtaaaca
    13921 ttaggcacaa aatcgccatt aaaatcagta agctgaccat tgtttggaat gaaatgaaca
    13981 ggtgaagcat atttgctccc gggttcaaca acttgtgcca tcatgtcggt gcactgcggt
    14041 acaagcagaa ttaacaatgc cagtgtgtgc agagttaggg tcgaaattgg gtatttcact
    14101 ttctgcatga tacaaaggtg atgcataccc atgacccgta ataggacaaa tatcaccaac
    14161 ttccacgaaa accggatttt ctcaaaagtt gaattttcag ctgaaacacc gactggtatt
    14221 tattttcagc cttaattaga attaatccaa agaataatta atcatcaatg cccttgcccg
    14281 ctaatatttt cggaacatat ttttcaattc ttgcaatgcg tgttgccgat tgttttgctg
    14341 atgaaaagtg cagtaaatat gcgcgctgcc gtccgggtgt taatgcctca aatgcttttt
    14401 tgagttttgt acttttattg agtgccgatt taaactcttc tgccattgaa aattcttttg
    14461 tcttttttaa aggcatttta acgccggatt tctcaacttc aattgcttcg aaaatatagg
    14521 ctttgagtgc tgtggttgtt ttctttattt cagaaagcga agtaaagcgc atttgtcttg
    14581 ccgtctgcac attttcagtt tgctgaatca ggagtttttg agcgtcttgt aatgcaactc
    14641 ctttgaagaa taagactgcg cagtattctt tgaaaatatg tatcaaaaca atattgcgac
    14701 catcaagcgt ataacatggc gttccccatt tcaactcttc ctgcaattgg cagcttaaaa
    14761 ttgtcttccg taatgcacgg atctcatttt cccatttgcc ggct
//
LOCUS       DAOQOU010000068        14781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2067_length_14781_cov_6.492123, whole genome shotgun sequence.
ACCESSION   DAOQOU010000068 DAOQOU010000000
VERSION     DAOQOU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2067_length_14781_cov_6.492123"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..856
                     /locus_tag="PLM90_07480"
     CDS             41..856
                     /locus_tag="PLM90_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89250.1"
                     /translation="MDSRKVPEVYFHVGLGKTASTWLQNKVFGKFQGVLYIHPTAYTQ
                     WRKAVVNTIYDKVLISREMDRQLEREVSAFAAVRPDTHAIIVMRTNAQWIASQYRRFL
                     KNGYCFTFEEFFDVEHDNGFWKRDDVFLFPKIEILQKYFAHPPLILTYDQLKQDPKEF
                     VLAMANYIGVQVDINAISFDKHHASYSEKQLKWMLQVGKYLFSKKIQDPSLPRWRKWI
                     RRRPRMWMCYIVLYSAYLIPNALISKQPLITKEALDKIDRFYANDWECVLRKM"
     gene            1019..1240
                     /locus_tag="PLM90_07485"
     CDS             1019..1240
                     /locus_tag="PLM90_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89251.1"
                     /translation="MREKLHNSKLHQPLKHFENDVAYIAGWLKKLKEDRRFILFTSSK
                     AQRAVDYILNQQIADVKKEIGPVADVSEK"
     gene            1926..2840
                     /locus_tag="PLM90_07490"
     CDS             1926..2840
                     /locus_tag="PLM90_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89252.1"
                     /translation="MKGNAILITFLILSITLFSQTNNFAPVGAKWWYDYNNSPIEGYT
                     TLEVVGDTLIADKNCRILSSIKYTKDISEIDPEINITSNGRYFIYSDSDKVYQYVFGK
                     FYKLYDFNALIGDNWEVAGVSSDGICDSTGIISIDSISNITINTFNLFEFYSNSVLDY
                     YWGFGIQPVIERLGSLNFLFPNPENCLFDYYKGGPLRCYYDVEFGYFQADSALACDYI
                     HTNISNPGELIFNLWPNPFNSELHIAAPYNTTNSLLRVFDITGNLIINTAIDNQYSSM
                     NFSDLRSGLYIFDFYTNSVHKYLKIVKL"
     gene            2837..3784
                     /locus_tag="PLM90_07495"
     CDS             2837..3784
                     /locus_tag="PLM90_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89253.1"
                     /translation="MKAPLLFNRRIPMTVLFCTVFYLTRIFAQSLDPICDGIFENESS
                     FLTSSCSYSSDAWVEKYGRQEYWIPDPSNYDYTPIKTIRVNIHLMQHESPTPIGNFDA
                     DNATELAMIYSLFENVNDLYSSVCEPSDPITCVCGSDCHISDTRIRFEIKGVYEHQDD
                     SHYANTTNLFSLYAVNPETELNYFFLGYEDGTANGACVGNPDYTNLFHNTYLYSFDIY
                     DNYLYNPYVMSSVPIHLAHELGHALGLLHTYLPYCCPESCDEYTTDYLDDVFGSGVGS
                     CLQVCNHDNGWSCDVYSTSNSCSNNMMGGTGNACYFSPE"
     gene            3794..6178
                     /locus_tag="PLM90_07500"
     CDS             3794..6178
                     /locus_tag="PLM90_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89254.1"
                     /translation="MQRALHLSHIRKYVDDCPVTTQELEINEDETWDFDIRIYNKTIR
                     VTSGNVLTITCKVYMPEDGNIIVEQGAELILDGGFISKSCNGLWQGIEVWGVGSGTAH
                     PSISAIYSGTYPSSSSDHGVVYIKDGSTIEFARNAITTSKYDDFGNAAYRGGIVVAHG
                     ANFINCKRSAEFMQYDYTPSNTDDDNISEFYRCNFEVNDNYPCSETFNTHVTMWDVDG
                     VIFIGNNFKDLRETCTNNGRGIYSIDATYTVGSDLCGGATPCDDHISTFEGFYKGVEA
                     ENANARPRDIVVGDVVFSDNYRGILLSNVNHSVLISNTFEVPDIPTTSLNAYGIYLED
                     CSHYQVENNNFTTFGTYDDASPYNAGIYVVNNSDAVTEVYRNTFENLEAGVRIQTENS
                     KLQLKCNTFSSVIEKHDIYVTNTGTLGTQGLCLSNIYPCPTRVNSPAGNTFSFDCNNS
                     EGDFKIYSGHPELKYRHHNETAFTPSCYTTGATFITLNSCSYSHDMGGGCIACQSTLP
                     EGGTGFRMDGSNVPSTARDLLSELEKTQESIEGLEEALAAVEYELEYDFEGVSDNDDL
                     FYLLNQKEQLLDDCSNSYFLEGKVDSALLVLESFSIEGSDQKVVQFELLSGDYGSAAA
                     DLDKINSASVENSDFKVLYGILINKAKEGSDILSLSDSDIATLESIAGKQSRSGVAAE
                     NLLSFATGAEYEEVFDTDIEVLEKQLMFHDITKVSIYPNPVSNVLFVDVQSMKEVAIY
                     NLLVYDMAGKKLFEAEIDGNAITSLNTASLPKGLLLIQVREDGQTIMIEKVVNQ"
     gene            6184..7683
                     /locus_tag="PLM90_07505"
     CDS             6184..7683
                     /locus_tag="PLM90_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89255.1"
                     /translation="MYRGELAVLLIIISIKGCLLGQTTFNKTYDFSHGIDQVYAVTTI
                     NDGYILVGSGYGNEVGAFFDIKIKYAKIDYEGNLVWQKFIGAPDTNFFVYPKSSIATL
                     DGNVIFCGSRQSLTTSEIHLIKINSTTGDTIFMKRFDNDDEIYGLQVRELNDGSLIIL
                     GYDSNDSFAFFFIKTTPDGQFVWDKRYGTTTEGYPTEFNIVDDTIYTVNGNYLCAPEG
                     YWIRRMDSNGNVISTNYFNGDCVGNGLKSETEGYYGAGAYYSVPPYQTFSYRTDADGN
                     VLWHYETSIDMDTLEYEQLFPDIVKELPNGDLIVAGYYSSNYLGSYIGLISKINIEGV
                     AYWERFYTATPDPSDDNIIYDIDFCIDGGYILAGSAFGAEESEGQNFWALKLDSVGCL
                     IPGCDTLDINITEYQYNVGNIEVYPNPVLESAIVRINLNSASSVNEVNYTVSDITGRI
                     IYKSINCASTRKSNEISFTIFKNDLQSGVLALKLYTRGYLIGYTKFVVE"
     gene            complement(8174..8674)
                     /locus_tag="PLM90_07510"
     CDS             complement(8174..8674)
                     /locus_tag="PLM90_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ89256.1"
                     /translation="MASVTFTTLIKKFGSMGEKTGWTYVEVPFEYADKLNPGVKTSYR
                     VKGKLDDLSVKQLALLPMGGGDFIFPLNTDMRKKLGKRAGNSLTLKINIDNDAFSFNE
                     DFMACLSDEPKALNFFETLTGSHQKYFSKWIDSAKTDETKAKRITKAVNALARKMGYA
                     EMIREK"
     gene            8771..9514
                     /locus_tag="PLM90_07515"
     CDS             8771..9514
                     /locus_tag="PLM90_07515"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMZ89257.1"
                     /translation="MDSNLLNPTPIPAMPRIGDNAPVFEAETTQGHINFPADYAGKWV
                     ILFSHPADFTPVCTTEFMTFATMEQEFQNLNCQLVGLSIDSIYSHIAWLRTIKEKIAF
                     RDMKNVEVTFPLIDDIRMEVAMKYGMIQPNESNTKAVRAVFFIDPVGKIRAIIYYPLS
                     TGRNFEELKRVLIALQTADAFGVATPADWKPGDDVIVPPAGSCGVAKERMQGKDEQVY
                     CVDWFFCMKPLEKEKIDTLFVRKNEIVLN"
     gene            complement(9585..12905)
                     /locus_tag="PLM90_07520"
     CDS             complement(9585..12905)
                     /locus_tag="PLM90_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89258.1"
                     /translation="MQRLLPICILLSSSLNLQAQSLVTALIQHGNDSQQTTSIVADPD
                     NYMVAAVNFWDDLDADPGVPVVNFNTAGTMDVAIIKLDPAGNYVWGKQLAGAGWNASS
                     DIACDALGNIFIFGYYNGTMDMDPGPGTSNITSAGGDDIFVGKYDTNGNLLWAYSIGG
                     TGTEQNYGFDVGPDNNTVLYGYFQNTVDFNPGGGTFNLTAAVGGSNFILKLNNNGTFN
                     YAVSMGSAYGNELKIDAANNIYCTGLFWSTVDFDPGVATYNLTAAGFTNDSYIQKLSP
                     TGGFLWAGKFSSSLADQGTCIAIDNINNTVSVGGFFEGTIDADPGIGTANIASGGYVD
                     AYVINLNASTGNYNWGKSFSGLGWQALHSMDVSSTGSVIVAGSFDQTADFNPGAGTFN
                     MTSAGSTDIFKVALNSDGSFSSAEQIGSNGQDQPICLAIDQEGAVLLTGFFENIVDFD
                     PGANVFNLNAAFTGWDGFIAKYCTVYTINNYVNICSGDSYFAAGANQTTSGDYYDYYT
                     PVEGCDSIVITHLTVNNPTVNLGPDANICAGTTFTLDAENTGADYLWNTGATTQTINI
                     TATGTYSVTVTDDAGCIAADAITVTVQAAPVLELGPTIHSCADEFVLLDAGNAGADYL
                     WNTGATTQTISVNTSGNYSVTVTNDFGCTTSDLVHVTVHALPVVDLLADEGICEGETL
                     LLDAGNPGSTFAWNTGATTQTITVSDADTYSVIVTNAWGCDASDAITITINPTPVIDL
                     GADISLCDGESIILDATADDATYLWNTGATTSTITVTETGDYNVEVSNIFGCSAVDNI
                     HVEVNPLPIVDLGEDAAFCEDEFMVLDAENPGSTYLWNTGETTSTIIVTDDGTYSVDV
                     ISAFGCVSSDTISITIHPLPVADLGADVTSCSNVPVLLDATTPFCLYAWSTGETTPNI
                     VVDESGEYWVTITNSFGCQITDSITVSMLALPEVDLELEPTICVQDPAFTLSGGTPSG
                     GTYFGDGIIDGIFNPAAAGTGTHNIYYTYTDANGCSDTAMVSVTVNICNGIADNNATA
                     ISVFPNPAMNDITINLPSNLTSAEYVFMQADGRAALSGTAQSNLQVIDISTLAAGYYT
                     LIIADEGNIISAPVFVTK"
     gene            13018..13410
                     /locus_tag="PLM90_07525"
     CDS             13018..13410
                     /locus_tag="PLM90_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89259.1"
                     /translation="MQKLRTTILIAGIILVSSSAFAAIQPDGTSLMHAWNIQSVELFG
                     IATTPDETMAGDMLDLNADMTFTAIVDGNALSGTYGIDQSNTWITLYISADNAMRIKV
                     IESDATHIKTTRVDGDGNQTIVTYTIAD"
     gene            13657..13980
                     /locus_tag="PLM90_07530"
     CDS             13657..13980
                     /locus_tag="PLM90_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ89260.1"
                     /translation="MERAAVNIYRTENSYEMLVFAPGRIREHFDVHMEGSELTVSYRP
                     PEGFPRPEWVRREYSRGGFVRQFTLGAGIDTAGIAATYVNGVLEIHLPFLKGEENAKH
                     KIEVK"
     gene            complement(14035..>14781)
                     /locus_tag="PLM90_07535"
     CDS             complement(14035..>14781)
                     /locus_tag="PLM90_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89261.1"
                     /translation="GTYSSRSSATSEGMYICSATNGKVNFMKFYNFVDFTEFFSYLPE
                     RTQEKIEKKKAKADDKGKEFSYNYYIAEHPIRKIGDNYLLVGEAYYPTYRQEAYTSYV
                     NGKPVTNYRTVFDGYQYTHATIAMFDSEGGKLWDQTFKMYPGTKPFTVKRFISETYND
                     DELKMVFASLNSIKSMSVGYDGKISNERSAEIIKVGNDDEKVKSAYSNIEYWYGNTFV
                     AYGNQLLKNSKESIGNKKRRVYYINKIVFE"
BASE COUNT         4535 a         2732 c         2937 g         4577 t
ORIGIN      
        1 gatttgcgtg caccccttat tttgctgaat ttcgccgtcg atggatagcc ggaaggtccc
       61 tgaagtgtat ttccacgtag gtttgggaaa aactgccagt acctggttgc aaaacaaggt
      121 attcggtaag tttcaaggcg tgctttacat ccacccaacc gcatataccc aatggcgaaa
      181 ggcggtagtg aataccatat acgacaaggt gttgatctca cgggaaatgg accggcagct
      241 tgaacgagaa gtgagtgcat ttgcagcagt gcgacccgat acgcatgcca tcattgtgat
      301 gcgtacaaac gctcaatgga tagcttcgca atatcgacga tttctaaaaa atggctattg
      361 tttcactttt gaggaatttt ttgatgtgga acacgataat ggattttgga aaagggatga
      421 tgtttttctt ttccccaaaa tcgagatttt gcaaaaatat tttgctcatc cacccttaat
      481 cctaacctac gatcaactca aacaagatcc taaagaattt gtacttgcca tggcaaatta
      541 cattggagtg caggtagaca tcaatgctat ctcatttgat aaacatcatg catcctacag
      601 tgagaagcaa ttgaaatgga tgttgcaggt agggaagtat ttattcagca aaaaaattca
      661 ggacccttca ctcccacgct ggcgaaaatg gatacgccgc cgaccgcgca tgtggatgtg
      721 ctatatcgtt ttgtattctg cctacctcat tcccaatgcc cttatttcca aacaaccact
      781 catcacaaaa gaagccctgg ataaaattga caggttttac gccaatgatt gggagtgtgt
      841 attgagaaaa atgtaaatga atttcttcag acaattttaa tagcatctag agccaagctc
      901 gcctctggcg ggtatctagt atctagagtc aagctcgcct cgcctctggc gggtatctag
      961 tatctagaat caagtatcaa gattcatacc ggcttagcga ctttgcccct ttgcgccttt
     1021 gcgcgaaaaa ttacataact caaaattaca tcagccatta aagcattttg agaatgatgt
     1081 ggcctacatt gccggatggc tcaagaaact gaaagaagac agacggttta tcctatttac
     1141 cagttctaaa gcacaacggg cagttgatta cattttgaat cagcaaattg ctgatgtgaa
     1201 gaaagaaatt ggccctgtgg ctgatgtgag tgagaagtga cactacccat ttattaatga
     1261 acacacttcc cttagatgtg ttcaacaagg gcctgctatc tgaaaatggt gcaggctttc
     1321 ttttatgact catgaacaca gaaatgaagg gtgaccgcat caaatgcact tcactatatt
     1381 gaaatgttgt ttcgctaaat agaagtagca ttttgcattg aaaaacagtt ggttcacact
     1441 ataccatttg gaaacatgaa tttgtcacct tatattcata tatgtaattt attaaattga
     1501 ttaacggttt tccgttaaat cactattaaa aattaaccat tacataacga ttattaaaac
     1561 ttaatagtaa taatttcata gattagataa aatcactgaa tttagtagca atatacagga
     1621 agaaataatt caaataaggt cttgttaagc ggaatgtcca atatgagtca gaagtaacaa
     1681 tgtcctttta tttatgaacg cccttcgcat aagtgtgttc aacaagggcc ttttatcaaa
     1741 cttttgataa aaagcccttg ttgaaaaaaa acctcaaaaa atttggaatt ctcgattgag
     1801 tagaatattt ttatattaaa tcctcaaatt ctcaaattct catcgtatga aagcatatca
     1861 tccctctaca tcatagcagt tttttaattt tcagtttaac aatataatct tttagactca
     1921 ttgaaatgaa aggaaatgcg atacttataa ctttcttaat tttaagcata actctttttt
     1981 ctcaaactaa taattttgct ccagttggag caaaatggtg gtacgattac aataattcac
     2041 ctatagaagg atatacaacc ttagaagtag ttggagatac tttaatagct gataaaaatt
     2101 gcagaatcct gtcttctata aaatatacaa aagacatttc tgagattgac ccagaaataa
     2161 atatcacgtc caacggaagg tattttattt attcagacag cgacaaagtt tatcaatatg
     2221 tatttggcaa attttacaaa ttatatgatt ttaacgcatt aattggtgat aattgggaag
     2281 ttgcgggtgt atcatcagac gggatttgtg attcgactgg aataatttct attgatagta
     2341 tttcaaatat tacaattaat actttcaatt tatttgaatt ttattccaat tctgtcctag
     2401 attattattg ggggttcggg attcaaccag ttattgaaag acttgggtca ttaaattttt
     2461 tatttccaaa ccctgaaaat tgtttatttg actattataa aggagggcct ctcagatgtt
     2521 attacgatgt tgaatttgga tacttccaag ctgattcagc attagcttgc gattatattc
     2581 atactaatat ttcaaatcca ggggagctaa ttttcaattt atggccaaat ccatttaata
     2641 gcgaacttca tatcgcagca ccatataaca caaccaattc tttactaagg gtatttgata
     2701 ttacagggaa cttaattata aacacagcca tagataacca atattcatca atgaatttct
     2761 cagacctcag gtctggatta tatatattcg acttttacac taattctgtt cacaaatatt
     2821 taaaaattgt aaaattatga aagccccact tttatttaat cgccgcattc ctatgacagt
     2881 gctgttttgt actgtatttt atttaactag aattttcgca cagtctctag atcctatttg
     2941 cgatggtata tttgaaaatg aatcgagttt tttaacttca agttgctcct attcatctga
     3001 tgcttgggtt gaaaaatatg gcagacagga atattggata cctgaccctt caaattatga
     3061 ctatacccct ataaaaacca ttagggtaaa cattcactta atgcaacatg aatctccaac
     3121 acctattggt aactttgatg cagacaatgc aacggaactt gctatgatat atagcttatt
     3181 tgaaaatgtt aatgacttat attcaagtgt ttgtgaaccc tcagacccaa ttacatgtgt
     3241 atgcggttcg gattgtcata tttcggacac aagaattaga tttgaaataa aaggtgttta
     3301 tgagcatcaa gatgacagcc attatgcgaa tacaactaat ttattcagtt tgtatgcagt
     3361 aaatcccgaa accgaactaa actatttttt cctcggatat gaagatggta ccgcaaatgg
     3421 cgcatgcgtt ggtaaccctg attatacaaa tttgtttcat aatacctatt tatattcatt
     3481 cgatatctat gataattatc tatataatcc gtacgtaatg agttctgtgc cgattcattt
     3541 agcccatgaa ttaggccatg ctttaggtct tctgcatact tatttgcctt attgttgtcc
     3601 tgaatcatgt gacgaatata caacagatta tttggatgat gtattcggaa gcggtgtagg
     3661 tagctgccta caggtttgca atcacgataa tggatggagc tgcgacgttt attctacatc
     3721 aaattcatgc tcaaataata tgatgggagg aaccgggaat gcatgttatt tttccccaga
     3781 gtaaattggg cggatgcaac gtgcgctcca tcttagtcat attagaaaat atgttgatga
     3841 ttgtccagtt acaactcaag aattagaaat caatgaagat gagacgtggg attttgatat
     3901 tcgaatatac aataaaacaa tccgggtaac ctctggtaat gtattaacga ttacgtgtaa
     3961 ggtttatatg ccagaggatg gaaacattat tgtggaacaa ggtgctgaat tgattcttga
     4021 tggtggattt atttcaaaat cttgtaatgg gctttggcaa ggtattgagg tttggggtgt
     4081 tggaagtggg acagcccatc cttcaatatc agctatatat agcggaactt atccttctag
     4141 ctcttcagat catggtgtcg tttatataaa agatggctca accattgaat ttgctcgaaa
     4201 tgctattaca actagtaaat atgatgactt tggcaatgca gcttatcgtg gtggtattgt
     4261 agtagcacat ggagccaact ttattaattg caaacggtcg gctgaattta tgcagtatga
     4321 ttacactcca tcaaatacgg atgatgacaa tattagtgag ttttatagat gcaattttga
     4381 agtgaatgac aactatcctt gttctgagac atttaataca catgtaacaa tgtgggatgt
     4441 tgatggagtt atttttattg gaaataattt taaggatttg cgagagacct gtacaaataa
     4501 tgggagagga atttacagta ttgatgcaac atatacggta ggaagtgact tatgtggtgg
     4561 cgctacgcca tgcgacgatc atataagtac atttgaaggt ttctacaaag gagtcgaagc
     4621 tgaaaatgca aatgctcgcc ctagggatat tgtagtagga gatgttgtat tctcagataa
     4681 ttacaggggc atcttgttat caaatgtcaa ccattcagta cttattagta atacttttga
     4741 agtgccagat attccgacca cgtcgttaaa tgcatatgga atttatcttg aggactgtag
     4801 tcattatcag gttgaaaata ataatttcac cacttttgga acctatgatg atgcatcccc
     4861 ctataatgca ggcatttatg ttgttaataa ttctgatgca gtaacagaag tttatagaaa
     4921 cacgtttgaa aatcttgaag cgggagttcg catccaaact gagaattcta aattgcaact
     4981 taaatgtaat acatttagta gcgtcattga gaagcatgat atttatgtta caaatacagg
     5041 gacattaggc acgcagggat tgtgcttaag taatatttat ccttgtccaa cgcgggtaaa
     5101 ttcaccagca ggtaatacat ttagttttga ttgtaataat tctgagggtg atttcaaaat
     5161 ttacagcggg catcccgaat taaaatatcg ccatcataac gaaacggcat ttacaccttc
     5221 ttgctatacc acaggagcaa cattcattac cttaaattcc tgctcatatt ctcatgacat
     5281 gggaggaggt tgtatagcct gtcaatcgac cctgcctgaa ggaggaactg gctttagaat
     5341 ggatggttcg aatgttcctt caactgcaag ggacttacta agtgaattgg aaaaaactca
     5401 agagtctatt gaggggctgg aggaagcatt agcagctgta gaatatgaac ttgaatatga
     5461 ttttgagggg gtgtctgaca atgatgattt gttctattta ctaaatcaaa aagaacaatt
     5521 acttgacgat tgctctaatt cgtatttctt ggaaggaaag gtagattcgg cattgttggt
     5581 gttagaaagt ttttctattg agggttcaga tcagaaggta gttcaatttg agcttctaag
     5641 cggtgattat gggtctgcgg cggccgattt ggacaaaatt aacagtgctt cagttgagaa
     5701 ctcagatttt aaagtgttat atgggatttt aattaacaaa gccaaggaag gctcagatat
     5761 tctctcgtta agtgattctg atattgcgac cttagaaagc atagcaggta agcaaagtag
     5821 aagtggtgtg gctgcagaaa atttactatc tttcgcaact ggcgcggagt atgaggaagt
     5881 ttttgataca gatatagagg ttttggagaa acagttgatg tttcatgata tcactaaagt
     5941 gagtatttac cccaatcctg tatccaatgt gttgtttgta gatgttcaat caatgaagga
     6001 ggtagccata tataatttgc ttgtttatga catggcaggc aagaaattgt ttgaagcaga
     6061 aatagatggg aatgcaatta cttctttgaa tacagcaagc cttccaaaag gacttttact
     6121 aattcaagtt agagaggatg gccaaacaat tatgattgaa aaagttgtaa accaataaaa
     6181 gcaatgtaca ggggagaatt agcagtatta ctcataatta tttctattaa aggttgtctg
     6241 cttgggcaga caacctttaa taagacttac gatttttcgc acggtataga ccaggtgtac
     6301 gcggtaacca ctattaatga tggctatatt ttagttggaa gcggatacgg aaatgaagtt
     6361 ggagcgtttt ttgatattaa aattaagtat gccaagattg attatgaagg aaatcttgtg
     6421 tggcaaaagt ttattggagc accagacact aatttctttg tatatcctaa aagtagtata
     6481 gcgactttag atggaaatgt gattttttgt ggaagcaggc aatccctcac tacttcagaa
     6541 atacatttaa ttaaaattaa tagtaccaca ggagatacaa tattcatgaa aaggtttgac
     6601 aatgacgatg agatctatgg acttcaagta cgtgagttga acgatggatc gctcatcatt
     6661 cttggatacg attcaaatga ctcatttgcc ttttttttta ttaagaccac acctgatgga
     6721 caatttgtat gggataagcg atatggtaca acgactgaag gttaccctac tgaatttaat
     6781 atagttgatg atacaattta tacagtaaac gggaattatt tatgcgctcc tgaaggatat
     6841 tggattcgca gaatggattc taatggaaat gtaatttcaa caaattattt caatggcgat
     6901 tgtgttggaa atggtctaaa atctgaaact gagggatatt atggagctgg cgcatattat
     6961 tcagtccccc cttaccagac tttttcatac agaactgatg cagatggaaa tgtactatgg
     7021 cactatgaaa caagtatcga tatggataca ttggaatacg agcaactatt ccccgatatt
     7081 gttaaggaac taccaaatgg cgaccttatt gtcgctggat attattcaag taattatctt
     7141 ggtagctaca ttggactaat aagtaaaatt aatatagaag gggttgctta ttgggaaaga
     7201 ttttataccg caacacctga tccttcggat gataacatta tatatgatat tgacttctgt
     7261 atcgatgggg gttacatatt agcaggttct gcttttggtg cggaggagtc tgaaggacaa
     7321 aacttttggg ctttgaagct tgatagtgtc ggctgcctaa tccctgggtg cgacaccttg
     7381 gatataaata ttactgagta tcaatataat gttggtaata ttgaagttta cccaaatccg
     7441 gtgttagaat cggcaattgt tagaattaac ctaaattcgg catcttcagt taacgaagtg
     7501 aattatactg tatctgatat tactggtcgt attatttaca aatcgatcaa ttgtgcttcc
     7561 actagaaaaa gtaatgaaat ctcattcacc atttttaaaa atgatttgca aagtggagtt
     7621 ctggcactga aattgtatac tcgcgggtat ttaattggat acacaaaatt tgttgttgaa
     7681 tagaaattag attggtgcaa ttgtgaatta taatattgtc taggtttata gtaatgtata
     7741 ctatatcgaa ttattaattg cgtcgcaagc aatagcaata gataactcat tgacctttct
     7801 tcctaaagtc actattagca aggttaaata ccaagcataa agaccctgta aattgattta
     7861 cgagtatgtg agtaatactt tgcacaaaag ttcaagaaat catttgagat atcgccaact
     7921 cacgtaagca tcaaatagca catccaattc catatatatg gacattccgc taaacaagcc
     7981 catttcaacg ctttattcag gcccaccggg aattatttta gttggtttca caactacaac
     8041 aaatttaatg aattgactat taattttaag tcaattgcgt tgggtctgca atttgtggaa
     8101 aagaggggtc tgaacttgcg aaatttccac ctagcgtctt taaagtatga aaaacgctca
     8161 agcaatcacc acatcacttc tcccgtatca tctccgcata tcccattttt ctggcaagcg
     8221 catttacggc tttggtaatt cttttagcct ttgtttcatc agttttcgca ctgtctatcc
     8281 attttgagaa atatttttga tgtgaaccgg tcaacgtttc gaaaaaattc aaggccttcg
     8341 gttcatcact caaacatgcc ataaaatctt cattaaatga aaatgcgtca ttgtcgatat
     8401 tgatcttcaa ggtgagggaa tttcccgcac gcttaccaag ttttttacgc atgtcggtat
     8461 tgagcggaaa tatgaagtcg ccaccaccca tgggcaataa tgctaattgt tttacagaca
     8521 gatcatctaa ttttcctttc acccggtacg aggttttgac accgggattc aatttatcgg
     8581 catactcaaa tggcacttca acataagtcc agccggtttt ttctcccata gagccgaatt
     8641 tcttgatcaa ggtggtgaaa gtcactgatg ccatactgat attaaacact ttaatgttgt
     8701 gttacaaata tcactcatta gcggcattga tcatacgaac tttgcatcat aaataaaaaa
     8761 ataacctaat atggatagta acttattaaa tccaacacct atccctgcca tgcccaggat
     8821 aggggataat gcccccgttt ttgaagctga aaccacgcag ggtcatatca atttcccggc
     8881 cgattatgca ggcaaatggg taattctatt cagccatcct gctgatttta cgcctgtttg
     8941 cactactgag tttatgactt tcgctaccat ggaacaggaa tttcaaaacc tgaattgtca
     9001 attggttggt ttgtctatcg acagcatcta tagtcatata gcctggttgc gaacaataaa
     9061 ggagaaaatt gcattccgcg atatgaaaaa tgtggaagtt accttcccgc ttatcgatga
     9121 tatacgcatg gaagttgcca tgaaatatgg catgatacaa ccgaacgaaa gcaatacaaa
     9181 agcagttcgg gcagtatttt ttattgatcc ggttgggaaa attcgcgcaa tcatttatta
     9241 tccattgtcg acaggaagga attttgaaga attaaaacgc gtgctcatcg cattacaaac
     9301 tgccgatgcc ttcggagttg ccactcctgc cgattggaag cccggtgatg atgtaattgt
     9361 tcctcctgca ggttcatgcg gagttgcaaa agagcgcatg cagggaaaag atgaacaagt
     9421 atattgcgtt gattggtttt tctgcatgaa accattggaa aaagagaaaa tagatacact
     9481 attcgtacgg aaaaatgaga tcgtgcttaa ttgatctcat ctttttgaat taaggtgtat
     9541 gttaagcagc atggctatgg ttcagtatgc catgctgctg ttttttattt tgtgacgaaa
     9601 acgggagcgg atataatatt cccttcatcc gcaataatga gcgtatagta gcccgcggca
     9661 agcgtagaaa tatcaataac ctgaagattg ctttgcgctg ttccgcttaa agcggctctt
     9721 ccatcggcct gcatgaaaac atattccgcc gatgtcagat tacttggcaa attgatggtg
     9781 atatcattca tcgccggatt tggaaatact gaaattgcag ttgcattatt atctgctatg
     9841 ccgttgcaaa tattcacggt aacagaaacc attgctgtat cactgcatcc attcgcatct
     9901 gtgtaggtgt aataaatatt gtgtgttcct gtgcctgcag cggcgggatt gaaaatgccg
     9961 tcaataatgc cgtcgccaaa ataagtacca cctgaaggtg ttcctccgct taatgtgaat
    10021 gccggatcct gaacacaaat tgttggttct aattccagat caacttccgg taaggccaac
    10081 attgaaacag taatgctatc tgtgatctgg caaccgaagg aatttgtaat ggttacccaa
    10141 tattcaccgc tttcatcaac tactatgttt ggtgtagttt ctcctgtact ccatgcatac
    10201 aaacagaatg gtgtggtggc atctaacagt accggtacat ttgaacaaga agttacatcg
    10261 gcaccaagat cagcaaccgg aagtggatga atggtgatgg aaattgtatc agaagataca
    10321 catccaaatg cagaaattac atccacacta taagtaccat cgtcagtaac aataattgtt
    10381 gatgttgttt caccggtatt ccaaagatat gtggaacccg gattttctgc atccagcacc
    10441 ataaattcat cttcacagaa tgccgcatcc tctccaagat caactattgg aagaggatta
    10501 acttctacat ggatattgtc gactgcagag catccaaaaa tattactaac ctcaacattg
    10561 tagtcgccgg tttcagttac ggtaattgtt gatgttgttg ctccggtatt ccataaataa
    10621 gtggcatcat ccgcagtggc atcgagtata atggattcac catcgcataa tgaaatatcg
    10681 gcaccaagat caataaccgg tgttggattt attgtaatgg ttatggcatc agaagcatca
    10741 catccccagg cgttggtaac tattacgcta tatgtatcgg catctgatac agtaatagtt
    10801 tgtgtcgttg ctcctgtatt ccaggcgaat gtagaacccg gatttccggc atccaacagc
    10861 agtgtttcgc cttcacagat tccttcatca gcaagcagat ctactacagg taaagcatgc
    10921 acagttacgt gaacaagatc tgaagtggta cacccaaaat cattggtaac ggtaacggaa
    10981 taattgccgc tggtattaac agaaatggtt tgagtggttg cccctgtatt ccacaaataa
    11041 tcggcacctg catttccggc atccagtaat acaaattcat cggcgcagga atgaatagta
    11101 gggccgagtt ctaaaacagg agcagcttgt actgttaccg ttatggcgtc agcagcaatg
    11161 cagcctgcat catcagtaac agttacagaa taagtgcctg ttgcagtgat atttattgtt
    11221 tgagtagtgg caccggtgtt ccataaatag tcggcacctg tattttctgc atccagtgta
    11281 aatgttgtgc ctgcgcaaat gtttgcatcc ggaccgagat ttactgttgg attatttacg
    11341 gtgagatgtg tgattacgat actatcacac ccttcaacag gtgtataata atcgtaataa
    11401 tcgccacttg ttgtttgatt tgcacctgcg gcaaaatagc tgtcgccgga acaaatgttt
    11461 acataattat tgatggtata caccgtgcaa tattttgcaa taaatccatc ccaaccagta
    11521 aatgccgcat tcaagttaaa tacatttgca ccgggatcga aatcaacaat attttcaaaa
    11581 aatccggtta gcaatacagc gccctcctga tcaatagcta aacatattgg ttgatcttgc
    11641 ccgttggaac caatttgttc ggctgaagaa aaggatccat cactgtttaa tgcaaccttg
    11701 aaaatgtctg ttgacccggc agaagtcata ttaaaggtac ctgctcccgg attaaaatcg
    11761 gcagtttgat caaacgaacc ggcaacaata acactgcctg ttgatgaaac gtccatcgaa
    11821 tgcagcgctt gccatccaag tccgctaaat gacttccccc aattataatt tcctgtcgat
    11881 gcattgagat ttattacata ggcatctaca tatcctccgg aagctatgtt tgctgtacct
    11941 atgcccggat cagcatcgat ggtgccttca aaaaaaccac ctacggatac tgtattattg
    12001 atattatcaa tagcaatgca agttccctga tcagcgaggc tactgctgaa tttccctgcc
    12061 cataaaaatc ctccggtggg tgataacttt tgaatataag aatcatttgt gaatccggct
    12121 gcagttaaat tgtatgttgc aacgcctggg tcaaaatcca cagtgctcca gaataatccg
    12181 gtgcaataaa tattattggc ggcatctatt tttaattcat ttccataagc agagcccata
    12241 gaaactgcat aattaaacgt gccgttatta ttcaatttta aaataaaatt tgaaccacct
    12301 acagcagcag taagattaaa cgttccgcca ccgggattaa aatctacggt attttgaaaa
    12361 taaccatata aaacagtgtt gttatctgga ccaacatcga atccatagtt ctgttccgtg
    12421 ccggttccac cgatggaata agcccaaagt aaatttccat ttgtatcata tttccccaca
    12481 aaaatatcgt caccgccggc agatgtaatg ttcgatgttc ccggtccggg atccatatcc
    12541 atggtgccat tgtaataacc gaaaatgaaa atatttccca aggcatcgca ggcaatatct
    12601 gacgaggcat tccaacctgc acctgccagt tgttttcccc agacataatt gcccgcaggg
    12661 tctaatttaa taatggccac atccatggtg ccggcagtat tgaaatttac caccggcacg
    12721 ccgggatctg catccaggtc gtcccagaaa ttaactgccg caaccatata attgtcggga
    12781 tcggctacaa tggatgtcgt ttgttgcgaa tcgttaccat gctgaatcag cgctgtaaca
    12841 aggctttgag cttgtaaatt aagggatgag gatagcagaa tacaaatggg taataatcgt
    12901 tgcataggca ggtttttgtg tgttgacgag tttggtagcc aagaattgtg ccaaggtgat
    12961 ttatataaat atattacctt tatactagat ctgattaaca tattataaaa attatatatg
    13021 caaaagcttc gtaccactat tctaattgca ggtattattt tagtttcatc ttctgccttt
    13081 gcagccatcc aacctgatgg tacttcattg atgcatgcat ggaatattca gtctgtagaa
    13141 ttatttggca ttgctacaac acctgatgaa acaatggccg gagatatgtt ggatttaaat
    13201 gccgatatga cctttaccgc cattgtggat ggtaatgcat tatcaggtac ttatggcatt
    13261 gatcaaagta atacctggat cacattatat atcagcgcag ataatgccat gcgaattaag
    13321 gtgattgaat ctgacgctac gcacattaag actacaagag tagatggaga tggcaatcaa
    13381 accatcgtta cctacaccat tgcagattga gtgtatgtgg tcttagctca tttgggaact
    13441 taattttctt ttttttgtta ttgctccctt attgcgttta tgcaatatga tgttgtgcaa
    13501 ttttaacttt tgtgcataca atgtaattta gctgtgctcg tctgtttagt ggataattat
    13561 tttttcacta aaaaatgtaa tcagatgtat tacacacaaa cctgtgccgc aaaaggcaca
    13621 tccggcaata acacgcggca gttattgtac cgatcaatgg aacgcgccgc agtaaacatt
    13681 taccgcaccg aaaacagtta cgaaatgctg gtgtttgcac ccggacgaat tcgcgaacat
    13741 ttcgatgtgc acatggaagg cagcgaactt accgtttctt accgcccacc tgaaggattt
    13801 ccgcgccctg aatgggtacg aagggaatac agtagaggtg gatttgtaag acagttcacc
    13861 cttggtgccg gcatcgacac tgcaggaatt gctgccactt atgtaaatgg tgttttggaa
    13921 attcatttgc cttttttgaa aggtgaagaa aatgccaaac acaaaattga agtgaagtaa
    13981 aaaacaaaag gctgacgtgt gatgcgtcag ccttttatat ttcttaacat tcaatcactc
    14041 aaacacaatc ttattgatat aatacacacg acgcttttta ttgccgatag attctttgct
    14101 gttttttaac aactgattgc cataggccac gaaggtgttg ccataccaat attcaatatt
    14161 agaatatgct gatttcactt tttcatcgtc gttgccaact ttaataatct cagcagagcg
    14221 ttcattggag atttttccgt catatccaac gctcatcgac ttgattgaat ttaagctggc
    14281 aaacaccatt ttaagttcgt cgtcattgta tgtttcagag ataaatcgtt tcacggtaaa
    14341 aggtttggtt ccgggataca ttttaaaagt ttgatcccac aatttaccac cttctgaatc
    14401 aaacatggca atcgttgcat gagtatattg gtaaccatca aacacagtac gataattagt
    14461 taccggcttt ccattcacgt aagaggtata cgcttcctgt ctgtatgtcg gataatacgc
    14521 ttctcctact aacagatagt tgtctccgat ttttctgatc ggatgctcgg cgatgtaata
    14581 gttgtagctg aactctttac ctttgtcatc ggccttggct tttttctttt cgattttttc
    14641 ctgggtgcgt tcaggcagat aagagaagaa ttcggtaaaa tcgacaaaat tgtaaaactt
    14701 cataaagttt accttgccat tagttgcact gcagatatac atgccttcag aggttgcgga
    14761 actacgtgaa gaataggtgc c
//
LOCUS       DAOQOU010000069        14776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2068_length_14776_cov_6.375926, whole genome shotgun sequence.
ACCESSION   DAOQOU010000069 DAOQOU010000000
VERSION     DAOQOU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14776
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2068_length_14776_cov_6.375926"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="PLM90_07540"
     CDS             <1..1688
                     /locus_tag="PLM90_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ89262.1"
                     /translation="QNVVIDSYVPPVANFTFSGEPTVSFVDASLNSPTSWFWNFGDGG
                     TSTLENPTHTYTYNGTFNVCMTATNATGSNTSCQFVVIDYYLAPVAAFTYSGDPAVSF
                     TDASLNSPTSWFWNFGDGGTSTLENPSHLYASNGVYNVCLTATNATGSNTSCQLITID
                     SYLSPVAAFTFTGDPTVTYTDLSTNSPTSWAWSFGDGGVSTLENPTHTFTTNGTYNTC
                     LTATNAVGSNTSCQDVIIDSYVYIPVADFTYTGDPDVEFTDISSNAPTTWAWDFGDGG
                     TSSLENPIHLYASDGTYDVCLTAGNIAGSDTECKTIIVTGYVAPSALFSFSGDPVTTF
                     TDLSTGSPNSWLWNFGDGGVSTLENPSHVYATNGIFNVCLSIVSPGGSDAYCTDVTIA
                     GNGSAPTIDFSYVINSPTVAFTDLSSDSPSDWLWDFGDGAISGLQNPTHAYAVEAVYE
                     VCLTATNDFGTNTDCKSINVTDNAIQVLDQITLNCYPNPAGTYTMIEGLPAGTLSGDI
                     HITSVTGQEVGDNSIISWDNGICIINVSKLAAGTYTVKMLVDNQLFTGQFVKL"
     gene            2132..5203
                     /locus_tag="PLM90_07545"
     CDS             2132..5203
                     /locus_tag="PLM90_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ89263.1"
                     /translation="MMKAFTISILLMIVTAGLSAQTTLVGNTTFDFQSYWANHNRLLV
                     YPDGKVSTAWVGSDGFGAAFNDRGMFYNHFNGTSWGAFPSGRLEDQKTYFGELLKVMG
                     HEVVISDETTRLLVNKNSTVGATDWTETAGSNVIDGYNPMAFCPEGTDDIYVVNTKKI
                     TYESLLFSRSDDGGETWAVNEYFLPFTEEAYGIELVNPDCYQIVVHGNDVYVLYGANF
                     CDLILMHSPSKGDPGTWTSQTLVNFPIDNYTGAAGQTSDYDGDGDKDTIYTTDGMHEM
                     IITDDGTVHVFSGRMRIYDPTTAAGYYYIPKTSGIYYWHTGMASATVLDVMVDWNNDD
                     GLDDPYAGLGSDFTAYYAESVTTMPTACYDPSSGRVYLMFMMPVEYEISAGNQTYYDI
                     FGCYSDDGGTTWTSPVNITYTAYLGHENAYASAFPKVMDGKIHLQWQQDEEPGTTQDS
                     PADDIVINNILYNAFDPSRFDPYNPTVDFTYTLIPVGMTYSATFTNLSVDAEEYYWEF
                     GDGGTSTLENPTHIYAPGIYNVCLTGYNVYGETTVCEEIIAMNEPVADFTYIGDPEVA
                     FIDLSTGSPAEWYWDFGDGFTSTESDPVHTFSDNGVYNVCLTVTNGAGSDTHCENVTI
                     DSYVAPTAYWIYSGDPTVTFTDLSIGSPTSWSWDFDDGFTSAIASPVHTYAVNGTYHV
                     CLTVSNGAGTSTSCQDVVIDSYLSPVVDFSFTGDPTVSFTDLSTNSPTSWDWDFDDGT
                     FSTVENPVHTFPTNGSYNVCLTATNGIGSGTDCKVVVINGYAAPVALFSYSGDPVVSF
                     TDLSTGTPTSWFWDFDDGAFSTVENPVHTYATDGIYSVCLTVTGPGGVNTGCQDVTIS
                     STAGAPTAAFSYINVGPLTIKFTDLSTNAPTDWSWDFGDGSISGLQNPSHTYANIGEY
                     NVCLTASNGIGEDTYCEVIFAGDDIQSLDINHVIAYPNPASSILNVNIPNVSGDHDVS
                     IINMLGQKLPVKELQFIENILTLNIAELPAGNYMVLVHQDGGNYITYFTKE"
     gene            complement(5474..6772)
                     /gene="cysN"
                     /locus_tag="PLM90_07550"
     CDS             complement(5474..6772)
                     /gene="cysN"
                     /locus_tag="PLM90_07550"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HMZ89264.1"
                     /translation="MEHLVQDGKFDHHGYLNMELLRFTTAGSVDDGKSTLIGRLLYDS
                     KSIFQDQLEQIEQTSKNRGEEYVNLALLTDGLRSEREQGITIDVAYRYFSTPKRKFII
                     ADTPGHIQYTRNMVTGASTANLAIILVDARNGVVEQTYRHSFIASLLQIPHVVICINK
                     MDLVDFSQEKYEEVVEQFRHFASKLDVQDVRFIPISALLGDNVVDRSKNMPWYEGPTL
                     MYTLETVHIGSDHNLIDCRFPVQYVVRPMSNEYHDYRGYAGRVAGGVLKPGDEVMHLP
                     SGITTKIAEIKSLDGDLEEAYPPQSVTLILEDDVDISRGDMLVRENNAPKTGQDIDIM
                     LCWMDDKKSLQLNGKYAIKHTTRDARCVVKEILYKVDVNTLHRIQDVDSLALNEIGRV
                     KIRTTVPLFYDEYRRNRNTGSIIIIDEATNTTVGAGMIIE"
     gene            complement(6864..7769)
                     /gene="cysD"
                     /locus_tag="PLM90_07555"
     CDS             complement(6864..7769)
                     /gene="cysD"
                     /locus_tag="PLM90_07555"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HMZ89265.1"
                     /translation="MSYSLTHLRELEAESIYVMREVAAQFQKPALLFSGGKDSIVMVY
                     LARKAFWPAKIPFPLVHIDTGHNFPETMEYRDWLVKEMGATLIVGSVEDSINRGTAVE
                     ERGLNASRNALQTVTLLETIESNKFDACMGGARRDEEKARAKERFFSHRDEFGQWDPK
                     NQRPELWNLFNGRKHIGEHFRVFPISNWTEMDVWQYIYQENIPLPRLYFAHDREVIER
                     DGAFISTHPEWVKLRPNETAKTMHVRFRTCGDLPITGAIESDADTVEKIIYEVATTRV
                     TERGNRADDKRSETSMEDRKKAGYF"
     gene            complement(7776..8384)
                     /gene="cysC"
                     /locus_tag="PLM90_07560"
     CDS             complement(7776..8384)
                     /gene="cysC"
                     /locus_tag="PLM90_07560"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HMZ89266.1"
                     /translation="MAPVQDNIHPIFHRLLQRSDKEQLLKQTAKVIWLTGLSGSGKST
                     IAEALEKELHKMGFLTMLLDGDNIRTGINNNLSFSAEDRIENIRRISEVSKLFLNCGI
                     ITINCFVSPTNEIRALAKSIIGEADFLEVYVNAPLEVCEARDVKGLYAKARKGLIPDF
                     TGITAPFEAPEHPALEIPTDKLEIQEGVQRLMDFILPIISNQ"
     gene            8632..9135
                     /locus_tag="PLM90_07565"
     CDS             8632..9135
                     /locus_tag="PLM90_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ89267.1"
                     /translation="MDFKLRPWNMKDLPDLVKHANNPNIAGNMTNGFPHPYTKADGIK
                     FIHFATQDDPVHIFAIEVEGHAVGGIGMHPQTDIHCKNAELGYWLSEDYWRNGILSKA
                     IPLMIDFAFKTYDIERLFARPFGSNKASQKVLEKNGFKLEARLEKTIFKNGIFEDELI
                     YAVRRKK"
     gene            9199..9654
                     /locus_tag="PLM90_07570"
     CDS             9199..9654
                     /locus_tag="PLM90_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89268.1"
                     /translation="MQKFLQTILVVIGSLGVVISCNSGYDYWELSEFNMQPDALKDMA
                     EIKLVYYSNGPAQVDNDGAYIHAIVTSVETGDTINILSPYLSEFSPEDGDKTFIYIGI
                     HSNIGKAVSSDWDLDKYTKVIRDPKFDAIAHNDHPTVIGSIASTEMPFQ"
     gene            9667..10191
                     /locus_tag="PLM90_07575"
     CDS             9667..10191
                     /locus_tag="PLM90_07575"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HMZ89269.1"
                     /translation="MTTTDLQYPVGKFQRPAEFNAARMPAFIDTIASFPEKLKATVLV
                     MSEAQLNAPYRPGGWSVRQVVHHCADSHMNSFIRFKLGLTEDNPTIKPYEEAAWAEMS
                     DYLKTDIAYSLMIIEGLHARWTSLLRNMSEADFQRTVFHPQQNREMSLFTLLALYAWH
                     CDHHLAHVQIVSRQ"
     gene            10242..10691
                     /locus_tag="PLM90_07580"
     CDS             10242..10691
                     /locus_tag="PLM90_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89270.1"
                     /translation="MKTLLWVSICICLITSCRYAYVKDYTGEYNFELYYLEYDSLGVA
                     YATDTLLCTGTISRYQEGDEANATTPIESYEIAADEKAWLTISYNNWTVICHVDESGA
                     IEPYSYGTSYTHSGEFTDDTHILLNTFWGGLGLYENIEVHGEKMLND"
     gene            complement(10779..12638)
                     /locus_tag="PLM90_07585"
     CDS             complement(10779..12638)
                     /locus_tag="PLM90_07585"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89271.1"
                     /translation="MKNPMSLIKKSTCVALLAFAFAPAFAQSDIADPAFSSDGEYLSM
                     LRSDYLWDNDFVDEAGSITYWLNEPTGTGTTNYILRVLADGTPDLTLGLFGIKALESA
                     LIENPCDLPGEENCHTTTDITKAISLPGGKFLIGGMSYESDPIFSSWFVAQYLADGTL
                     DASFSDNGVIFGPIGKIHSVTGLGIDADGKILVLIEDYSSERSEIMRLNADGSFDDTF
                     GIDGLSILPDFTINCNDDIKTDASGNIYLAGNNSMPGDIVRLTSDGALDMSFGDEGRV
                     AFDTIDFHIEAFRIDDAGIVAAGEIELFVPSQFCLLRFDLDGNPDLSFSGDGHQFYIP
                     DSITPCPNYISSAAVISEANEFYISLNENAPDHYTSPIIFKFDADGNIDLSFSELGWT
                     WLSDAINRDIKLDADQRLIVSSEQYDALFGDHYIQIHRLLTDGTITGTEALLPCMIDE
                     VCNGIDDNENGIVDEDLPMNVFYADLDGDSYGNPEAPAVTCALELPGYVMENTDCDDT
                     NALINSGAEELIDGLDNNCNGLVDEGTDVHNMQVQFAIYPNPTDGILYIETNNSTEGL
                     LTIVNATGQEVLRYKTPLTGPTIDLTSLPDGMYIVNVCYQNVCDAQLLVLSRN"
     gene            complement(13002..14387)
                     /locus_tag="PLM90_07590"
     CDS             complement(13002..14387)
                     /locus_tag="PLM90_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ89272.1"
                     /translation="MRKFFQLLGQALKGENIDTTKGKLDTVIFLLAVPMIFEMGMESL
                     FAVVDIFFVSKVGVEAVAVVGLTESTLTIVYSIGFGLSMGATAMVARRVGENNPKGAA
                     EAAMQAIYIGLAIAAAVSLVGIFFPENLLRAMGASDQMIQNGVGYTRWMLTGNFAIVL
                     LFLNNGIFRGAGDAGIAMRSLIISNGINIVLDPLFIFGIWIFPELGVQGAAVATTIGR
                     SIGVIYQFYHLTNGKSLVHLKGKIWQLKTDIVRRIVILSSGSAGQFIIASASWIFLMR
                     IMSQFGEETLAGYTTAIRILVFSILPAWGISNAAATLMGQNLGANQPERAEKGVWRTA
                     YFNMLFMAVVTVIFVGFPEFFMRIFNSDDAVVNQGVTCLRIVCIGYIAYGYEMVLANA
                     INGAGDTRTPTIINVFGFWILQIPLAWFMAIQLDLGSPGVYAAVPISEAVMAVIYVYI
                     FRQGKWKLVKI"
BASE COUNT         4408 a         3178 c         2910 g         4280 t
ORIGIN      
        1 gtcaaaatgt ggtaattgat tcttatgtac ctcctgttgc caattttaca ttcagtggtg
       61 aaccaacggt gtcatttgtt gatgcatcat tgaacagccc aactagctgg ttctggaatt
      121 ttggtgatgg tggcacttct actttagaaa acccaacaca tacttacacc tataatggaa
      181 catttaatgt atgtatgaca gcaacaaatg caaccggttc aaatacttca tgccaatttg
      241 tggttattga ttattatctc gcgcctgttg ctgcatttac ctacagcggc gatcctgcag
      301 ttagctttac agatgcatca ttgaatagcc ctacaagctg gttctggaat tttggtgatg
      361 gcggcacttc tactttagaa aatccatctc atttatatgc atcaaatggc gtatataatg
      421 tttgcttaac tgcaacaaat gcaaccggat ctaacacatc atgtcaattg attaccatcg
      481 attcatattt atcaccggtg gctgcattta catttacagg tgacccaacc gtaacttaca
      541 cggatctttc aacaaacagt ccgacaagct gggcatggtc gtttggagat ggtggtgtaa
      601 gtacactgga aaatccaaca cacaccttca caaccaatgg aacttataat acatgtttga
      661 ccgcaacaaa tgccgtagga agtaacacct cttgtcagga tgtaattata gattcatatg
      721 tctatattcc ggttgccgac tttacttaca ccggtgatcc tgatgttgaa tttactgata
      781 tcagttcaaa tgcacctaca acatgggcat gggattttgg tgatggcgga acttcttcct
      841 tagaaaatcc tattcactta tatgcttctg atggcaccta tgacgtatgt cttacagccg
      901 gcaatattgc aggttcagat acagaatgta aaacaattat tgttacaggc tatgttgcac
      961 caagcgcatt gttctcattc agcggtgacc cggtaactac atttaccgat ctctcaaccg
     1021 gcagtccaaa ttcatggtta tggaattttg gcgacggcgg cgtaagtaca cttgaaaatc
     1081 catcacacgt ttacgcaaca aatggaatat tcaatgtttg cctgtcgatt gtgagtccgg
     1141 gaggttctga tgcatattgc actgatgtaa ccatcgcagg taatggatct gctcctacaa
     1201 ttgatttctc atatgtaatc aattcaccaa ctgtagcttt cactgatctt tcaagcgatt
     1261 cgccatcaga ttggttgtgg gattttggtg acggagcgat ctcaggactt caaaacccaa
     1321 cacatgctta tgcagttgaa gctgtatatg aagtttgctt gaccgcaaca aatgattttg
     1381 gaaccaacac tgattgtaaa tcaataaatg taacggataa tgctatacag gttctggatc
     1441 aaatcacatt gaattgttat ccaaatcctg ccggaacata tactatgata gaaggactgc
     1501 ctgcaggcac actttcaggc gatattcata ttacgtctgt aactggacag gaagttggcg
     1561 acaacagtat aatcagctgg gataatggca tttgcattat caatgtaagc aaactagccg
     1621 ccggcaccta cacggtgaag atgctggttg ataatcagct gtttacagga cagtttgtta
     1681 aattgtaatt ccaacaatat cattgaaagg cgagacaatg tctcgccttt tttattttgc
     1741 aaaattcatt gggtaaatcc ttattattaa ttaggaactt ccggagcaac ataccaatgc
     1801 agcagtatat gtaagctcag taaattgaca agagatcaac tttagtgaca aggtagaatg
     1861 gcatattaat aattgtaagg ttaacgtttc ttcgctacct tcaggtgtac atactgttaa
     1921 aattatcatc gaaaactatt tatatcgtgg taatattata aaattttaaa taatttaaaa
     1981 tcttgaagaa ggcggacatt gttccgcctt ttttttgcaa atatttatca aaatgtgacc
     2041 gccgtaacct gaaagcataa tacttaaata aacacgctct gtatcctatg gtgtaagttt
     2101 atatacagtt tcttacactc aatagtatac tatgatgaaa gcttttacta tcagcattct
     2161 cctcatgatt gtaacagccg gcctttcggc gcaaaccacc ttggttggca acacaacatt
     2221 cgattttcag tcttattggg caaatcacaa ccggctgttg gtatatccgg atggaaaggt
     2281 cagcaccgcc tgggttggat ccgacggatt tggcgcagcg ttcaatgatc gcggaatgtt
     2341 ttataatcac ttcaacggaa cctcttgggg tgcatttcca agcggaagac tggaggatca
     2401 aaaaacatat tttggcgagc tattaaaggt gatgggccac gaggtcgtta tttctgatga
     2461 aactacgcgt ttgctcgtaa ataaaaacag cacagtagga gccaccgact ggacagagac
     2521 tgccggttca aatgtaatag acggatataa tccgatggca ttttgccctg aaggtaccga
     2581 tgacatttac gttgttaata caaaaaagat cacctacgaa tcgttactat tttcccgttc
     2641 cgatgatgga ggcgaaacgt gggcagtaaa tgaatatttc cttccattca ctgaagaagc
     2701 atacggcatc gaattagtaa atcccgattg ctatcaaata gtagttcatg gcaatgatgt
     2761 ctacgtactt tacggagcta atttttgtga tttgatttta atgcattctc catcaaaagg
     2821 tgatccggga acatggacat cacaaacctt agtaaatttt cctatcgata attataccgg
     2881 tgctgcaggt caaacttctg attacgatgg cgatggagat aaagacacca tttacacaac
     2941 tgatggcatg catgaaatga taataacaga cgatggcact gtgcatgtat ttagtggaag
     3001 aatgcgcatt tatgatccta ccactgctgc cggatattat tacattccca aaacaagtgg
     3061 catatattat tggcatacag gtatggcctc cgctacagtg ttggatgtga tggttgattg
     3121 gaataatgac gacggcctgg atgatccata tgcaggtttg ggaagtgatt tcactgcgta
     3181 ttatgcagaa agtgtcacta caatgccaac tgcatgttac gacccatctt ccggcagagt
     3241 gtatttaatg tttatgatgc cggttgaata tgaaatatcg gccggcaatc aaacgtacta
     3301 tgatatattc ggatgctatt ctgatgatgg tggcaccacg tggacatcac ctgtaaacat
     3361 tacatacact gcttatctgg gacatgaaaa tgcatatgca agtgcatttc caaaagtaat
     3421 ggatggaaaa attcacctgc agtggcaaca ggatgaagaa cccggaacca ctcaggattc
     3481 tcctgctgat gatattgtaa tcaataatat tttatataat gcattcgatc cttcgcgatt
     3541 cgatccatat aatccaacag tcgattttac atataccttg attcctgttg gaatgacata
     3601 ttctgcaaca tttacaaatc tctctgttga tgcagaagaa tattattggg aatttggcga
     3661 tggtggcacc tctactcttg aaaatccaac gcatatttat gctccgggta tatacaatgt
     3721 atgtctgaca ggatataatg tttatggtga aactacagtg tgtgaagaaa taatcgccat
     3781 gaatgagcct gtagctgatt ttacttacat cggcgatcct gaagttgcat ttatagatct
     3841 ttctaccggc tcaccggcag aatggtattg ggattttggc gatggattta cgagcactga
     3901 atccgatcct gttcatacat ttagtgataa tggcgtttat aatgtttgtc tgactgtaac
     3961 aaacggtgca gggtcagata cacattgcga aaatgttact atcgattcat acgttgcacc
     4021 aacagcttac tggatttata gcggcgatcc tactgttaca tttacagatc tttccatcgg
     4081 ctcaccaaca tcctggagtt gggatttcga cgatggattt acctctgcca ttgccagtcc
     4141 ggtacataca tatgcagtaa acggcaccta ccatgtttgc ttaacagtat ctaatggagc
     4201 aggcacgagc acttcatgtc aagatgtggt aattgacagc tatttatctc cggttgtcga
     4261 cttcagtttc accggtgatc caactgtgtc atttacagat ctttctacca atagtccaac
     4321 atcgtgggat tgggattttg atgatggcac ctttagtacc gttgaaaatc ctgttcacac
     4381 atttcctacc aatggaagct ataatgtttg tttaacagct acaaacggaa ttggctccgg
     4441 aaccgattgt aaggttgtgg taattaatgg atatgctgca cctgtggcat tattctcata
     4501 cagtggcgat ccggttgttt catttactga tctctcaacc ggaactccca cctcctggtt
     4561 ctgggatttt gatgatggtg cattttctac agtcgaaaac cctgtgcata cctacgccac
     4621 ggatggaata tatagcgttt gtctcactgt aacaggtccc ggaggagtaa ataccgggtg
     4681 tcaggatgta actatttcaa gtacggcagg cgcgccaaca gcagcatttt catacatcaa
     4741 tgttggacca ttaaccatta agtttaccga cctgagcaca aatgcaccaa ccgattggtc
     4801 gtgggatttt ggcgatggaa gtataagtgg attacaaaat ccatcacaca cgtatgccaa
     4861 tatcggagaa tataatgttt gcttaactgc gtcaaatgga attggtgagg atacgtattg
     4921 tgaagtaatt tttgcaggcg acgatattca atcgttggac ataaaccatg tgattgctta
     4981 cccaaatccg gcgtcatcaa tattgaatgt caatattccc aatgtatcgg gtgatcacga
     5041 tgtttcgatc attaatatgt tggggcaaaa actgcctgtg aaggaattgc aattcattga
     5101 aaatattcta accctaaata tcgcagagtt gcctgcggga aattatatgg ttttagtgca
     5161 tcaagatggt ggtaattaca ttacttattt cactaaagaa tagattgatt ttatgatata
     5221 gaaaaagctc gtcatggttg gcgggctttt ttttgtgggt tgaaattccg gtcaaaatcc
     5281 tccctgatag acctttcgtg tttaagacct aacaggtcta agacctgtta ggtcttaaac
     5341 acgaaaggtc ttagacgaga aaggtttaaa acgagtaacc gcttctactc gaatcaaacc
     5401 gtttatgcac aaccacaaaa aaagccccga tgaatactct ccatcagggc tttggtttta
     5461 ggtttggtca atatcactca atgatcatac cggcgcctac tgttgtattg gtcgcttcat
     5521 caataatgat gatactaccg gtattcctgt tgcgacgata ctcgtcataa aataatggaa
     5581 ctgttgtgcg aatctttaca cgcccaatct cattcaatgc caatgaatct acatcctgaa
     5641 tgcgatgtaa cgtattcaca tctactttat ataaaatttc tttcactaca catcgtgcat
     5701 cgcgtgtagt atgcttgatt gcatatttac cattcaattg cagcgatttt ttatcgtcca
     5761 tccaacataa catgatatcg atatcttgtc cggtcttagg tgcattattt tcgcgcacta
     5821 acatatcacc gcggctgata tctacatcat cttcaagaat caatgtaacc gactgcggag
     5881 gatatgcttc ctctaaatcg ccatcaaggg atttaatttc tgcaattttt gttgtaatgc
     5941 cactgggtaa atgcatcact tcatcgccgg gcttcaatac accccctgca acacgaccgg
     6001 cataaccgcg ataatcgtga tattcattgc tcatcggacg aacaacatat tgaacaggga
     6061 aacgacaatc aattaaatta tggtcgctgc caatatgcac agtctccaat gtatacatca
     6121 acgtcggacc ttcataccaa ggcatgtttt tactgcgatc cacaacatta tcacccagga
     6181 gcgcactgat tggaataaat cgaacatcct gcacatccaa cttcgatgca aaatgacgga
     6241 attgctcaac aacctcttca tacttctcct gactaaaatc aacaagatcc attttgttga
     6301 tacaaatcac aacgtgaggt atttgcaaca acgatgcaat aaatgaatgt cggtaagtct
     6361 gctcaacaac accattgcgc gcatcaacaa gaataattgc aaggtttgcc gttgaagctc
     6421 ctgtaaccat attccttgta tattgaatat gtcccggagt atcagcaatg atgaatttcc
     6481 ttttcggggt gctgaaatag cgataagcta catcaatcgt aataccttgc tcacgttcgc
     6541 ttcgaagacc gtcggtaagt aaggcaagat ttacatattc ttcaccgcga tttttacttg
     6601 tctgctcgat ctgctccaac tgatcctgaa agattgactt tgaatcatac agcaagcggc
     6661 ctatcaaagt agattttcca tcgtcaacac ttcctgctgt agtaaagcgg agtaactcca
     6721 tgtttaaata accatggtga tcaaattttc cgtcttgaac taaatgttcc attgtaatga
     6781 ttatacaaat taaattatgt gtttactttt tttgtgattg cgaataaatc tattttattc
     6841 atacgtaatc acagggattt tatttagaag tatcctgcct tcttcctgtc ttccattgaa
     6901 gtctcactgc gcttgtcatc agcacgattt ccgcgctctg ttacacgagt tgtagcaact
     6961 tcgtagatga ttttttctac tgtatctgca tcactttcaa tagcacctgt aataggaagg
     7021 tcgccacaag tacggaaacg cacatgcatg gttttcgcag tctcgttcgg acgcaattta
     7081 acccattcag gatgagtcga aataaatgct ccgtcgcgtt caattacttc gcgatcatgc
     7141 gcaaaataca aacgcggaag tggaatattt tcctgataaa tatattgcca tacatccatc
     7201 tctgtccagt tagaaattgg gaacacgcgg aagtgctcac caatatgttt gcgaccattg
     7261 aacaggttcc ataactccgg acgctgattc ttcggatccc attgaccgaa ctcatctctg
     7321 tgagagaaga aacgctcttt tgcacgagcc ttttcttcat cgcgacgcgc gccacccata
     7381 catgcatcaa acttatttga ctcgatggtc tccagcagtg taaccgtttg aagggcattg
     7441 cgacttgcat tcaaacctct ttcttcaacc gcagttcctc tgttgatgga atcttcaaca
     7501 gatccaacaa ttaatgttgc acccatttct ttcaccaacc agtcgcgata ttccattgtt
     7561 tcagggaagt tatgtccggt atcgatatgc accagtggga acgggatctt agcaggccaa
     7621 aaggctttgc gagccaggta aaccatcact atagagtcct tgccgccgga gaataataaa
     7681 gccggttttt gaaattgtgc cgctacttcg cgcatcacat atatgctctc cgcctccagc
     7741 tcccttaaat gtgttaagct ataactcatg gtaaattatt gattcgatat aataggtaag
     7801 ataaaatcca ttaaacgttg cacgccctcc tgaatttcta atttatcagt gggtatttcc
     7861 aaagcaggat gttccggtgc ctcaaaaggt gccgttattc cggtgaagtc gggaattaac
     7921 cccttgcgag ccttggcgta taagcctttc acgtctctag cttcacaaac ttcaagcggt
     7981 gcatttacat atacttccag aaaatccgcc tcgccaatta tgcttttcgc caatgccctg
     8041 atctcatttg tcgggctcac aaaacaattg atggtgatga tcccgcaatt cagaaacagc
     8101 ttgctcacct ccgaaatccg acgaatgttc tctattctgt cttcagcgct gaaactcagg
     8161 ttattgttaa tccctgtcct gatattatcg ccatccagca acatggtcag aaaccccatt
     8221 ttatgcagtt ccttctccaa tgcctctgcg atagtgctct ttcctgaacc gcttaaaccg
     8281 gtcagccata tcactttcgc ggtctgtttc agcaactgct ccttgtcgga acgctgaagc
     8341 aaacggtgaa aaatcggatg tatgttatcc tgtacaggcg ccattgtgtg caaaaatcgc
     8401 tgcaaaggta aggaatctga cgtaaaaaat cagttttttc gaggccttga ggggtgataa
     8461 tggtcagtcc cgggaagcct attccactcc cgataggtct actttctgcc tcggtttagg
     8521 gggatggcgg gagttttttg ataaaagcta ccttttcacc aagccgaagc tcattgaaac
     8581 ctcaaatctt catattttcc tgcaacatct tgaaactgag tatctttgat aatggacttt
     8641 aaactccgcc catggaacat gaaagacctg ccggatctgg ttaaacatgc caataaccca
     8701 aatatagcag gcaacatgac caatgggttt ccgcacccct acactaaagc tgacgggatc
     8761 aaattcatcc actttgccac ccaggacgac cctgtccaca tctttgctat cgaggtggaa
     8821 ggtcatgctg tcggtgggat cggcatgcac ccgcagaccg atattcattg caagaatgca
     8881 gagttgggat attggctgtc ggaagattac tggaggaacg gtattttgag caaagccatt
     8941 cctcttatga tcgatttcgc tttcaaaaca tatgatattg aacgcctttt cgcccgacct
     9001 ttcggtagta ataaagcttc acaaaaagtg cttgagaaaa acggattcaa gctcgaagcg
     9061 cgcctggaga aaacaatttt taagaatggt atttttgaag atgaactaat ttatgcggtc
     9121 aggcgtaaaa aatgacatac tgcaaacaac cctacctgca tcattcacgt taatcaacca
     9181 aacaaccaat cgcctcccat gcaaaaattc ctccaaacta tcttagtagt tatcggcagt
     9241 ctgggtgttg tcatttcatg caatagtggt tatgattatt gggaattaag cgagtttaat
     9301 atgcaacccg acgcactaaa ggatatggcc gaaattaaat tggtgtatta tagcaatggt
     9361 cccgcacagg tagataatga tggcgcgtat atacatgcca tagtcacttc agtggaaacg
     9421 ggggacacca tcaatatttt aagtccgtat ttgagtgaat ttagtccgga ggacggtgat
     9481 aaaactttta tttatatcgg tatacattca aacatcggca aagcagtatc aagtgattgg
     9541 gatctcgata agtataccaa agttatccgt gatccgaaat ttgatgctat agcacataat
     9601 gatcacccga ctgtaattgg ttccattgct agcactgaaa tgccatttca ataaaccgcg
     9661 aaagatatga ccactactga tctgcaatac cctgttggca aatttcaacg tcccgctgag
     9721 ttcaatgctg cccgtatgcc tgcatttatt gataccattg caagctttcc tgaaaaactg
     9781 aaggcaactg ttttagttat gtctgaagcg caactcaatg caccttacag acccggtggt
     9841 tggtcagttc gacaagtggt acatcattgt gcggatagcc acatgaatag ttttattcga
     9901 ttcaaactgg gactcacaga ggacaatcca acaataaaac cttacgagga agcagcttgg
     9961 gctgaaatgt cggattatct caaaacagat atcgcatatt ccctgatgat cattgaagga
    10021 ttgcatgcca gatggacctc gctattgcgc aatatgtcag aagctgattt tcaacgcacg
    10081 gtatttcatc cacaacaaaa cagagagatg tcattattca ccttactcgc actttatgcc
    10141 tggcattgtg atcatcattt ggcgcatgtg cagattgtaa gtcggcaata atgaatatct
    10201 aattacaaat caccttactt cctcacataa aatgctcgaa tatgaaaaca ttgctttggg
    10261 tatccatttg tatctgtttg attacatcct gccgctacgc gtatgtcaaa gattataccg
    10321 gtgagtacaa ttttgaacta tactatttgg aatatgacag tttaggcgta gcctatgcca
    10381 cagacacact gctatgcacc ggcaccatat cgcgatatca agagggcgat gaggcaaatg
    10441 ccacaacccc aatagaatca tacgagatcg ctgccgacga aaaggcttgg ctcactattt
    10501 catataacaa ttggacagtg atatgccatg tagatgaatc aggtgcaatt gaaccgtatt
    10561 cctatggaac aagctatacg cattcaggag aattcactga cgatacacat attctgctaa
    10621 atactttctg gggtggcctt gggttatatg aaaatattga agtgcatggt gaaaaaatgc
    10681 tcaatgatta attggcagat cgagatacac taaaataata gacaaaaaaa atacaccgga
    10741 cttacattaa acctggtgta ttttttttaa tacatttatt aatttctgct aagcactaac
    10801 aattgcgcat cgcaaacatt ctgatagcaa acatttacaa tatacatacc gtcaggaagg
    10861 gaggtcagat caatagtggg accggtaagt ggggttttat atctaagcac ttcctggcct
    10921 gttgcattta caatagtcag caatccctct gttgaattgt ttgtttcaat atataatatt
    10981 ccatctgtgg gattaggata aatcgcgaat tgcacttgca tattatgcac atcagtgccc
    11041 tcatctacga ggccattgca attattatcg agaccatcaa ttaattcctc agcaccactg
    11101 ttaatcaggg catttgtatc atcgcaatca gtattttcca tcacgtagcc gggcaactcc
    11161 aatgcacagg taaccgccgg cgcctcaggg tttccgtaag agtctccatc taagtcggca
    11221 tagaagacat tcataggaag atcttcatcc acaatcccat tttcattatc atctatcccg
    11281 ttgcaaactt catcaatcat acatggcaac agtgcttcag tgccggtgat agttccatct
    11341 gtcagcaaac gatgtatctg aatatagtga tcgccaaata atgcatcata ttgctctgaa
    11401 ctaacaatca atcgttgatc agcatcaagc ttaatatctc ggttaattgc atccgacaac
    11461 catgtccatc ccaattctga aaacgacaga tctatatttc catccgcatc aaatttgaaa
    11521 ataattggtg aagtataatg gtcgggagca ttttcattca gactgatata aaattcattt
    11581 gcttctgaaa tcacggcagc tgacgaaatg taatttggac aaggtgttat tgaatccgga
    11641 atataaaatt gatgcccgtc gcccgaaaat gacaaatcag gattgccgtc aagatcaaat
    11701 cgaagcagac agaactgcga tggaacgaat aattctattt ccccggcagc tacaattcct
    11761 gcatcatcaa ttcggaaagc ttctatatga aaatcaatgg tatcgaacgc gaccctgccc
    11821 tcatcaccaa aactcatatc cagtgcacca tctgaggtta gtcttacaat atcgccgggc
    11881 atactattat ttcccgcaag atagatatta cccgatgcat cagtttttat atcatcattg
    11941 cagttgatcg taaaatccgg caatatagaa agcccatcta taccaaaagt atcatcgaag
    12001 gaaccatctg cattcaaacg catgatttca gatcgctctg atgaataatc ttcaatcaaa
    12061 acaagaattt ttccatccgc atctattcca agtccggtta cactgtgaat ttttccgatt
    12121 ggaccaaata ttacaccgtt atcgctaaaa cttgcatcaa gtgttccgtc agccaggtat
    12181 tgtgctacaa accatgaaga gaaaattgga tcagattcat acgacatacc tccaatcagg
    12241 aacttacctc cgggaagaga aatagccttt gtgatatctg tagtagtatg acaattttcc
    12301 tcgccgggaa ggtcgcaggg attttcaatt aatgcacttt ccaatgcctt aatcccaaac
    12361 aaacccaagg taagatcagg cgtgccatct gcaagtacgc gcaaaatgta attcgtagtt
    12421 cctgttcctg taggttcatt cagccaatag gtaatgctgc ctgcttcatc gacaaaatca
    12481 ttatcccaca agtagtcgga tcgcaacatg gaaaggtatt caccatccga ggaaaatgcc
    12541 ggatcagcaa tatccgattg agcaaatgca ggtgcaaagg caaaagcaag caatgctacg
    12601 caggttgatt ttttaatgag tgacatggga tttttcatgc gcagatattt tggggtttat
    12661 attgaattaa tgctaatatg aaataaaatt caaattatca ttatttacta aactacgggt
    12721 ttaaacccgt ttttcatagg agaatcggaa aaaaattggc attttttcgt tttcatctcc
    12781 cacaaagcca aaattcaacg gtaattacta gaactcatct caaaaatagg tttgatttag
    12841 cattttcaag tattcgcgct ttagccgagg cgcttgaagt cgcccctact ggaggttgta
    12901 agggcaattc aagtaacgat ggataaagag cgaagacgca gaaaagacgc caaaggtatt
    12961 tttgagatga gttctacata caatcacaaa aagataatga attaaatttt taccaatttc
    13021 cactttcctt gtctgaagat atagacataa ataacagcca tcactgcttc agaaataggc
    13081 acagcggcat atacccctgg cgagccaagg tcaagctgaa tagccataaa ccaggctaat
    13141 ggtatttgca atatccagaa tccaaataca ttgatgatgg taggtgttcg agtatcacct
    13201 gcaccgttta tggcattggc cagaaccatt tcatatccat aagcgatata tccgatacaa
    13261 acaatccgca agcatgtaac gccctgattt acaacggcat catctgaatt aaatatgcgc
    13321 atgaaaaact caggaaaccc gacaaaaatc actgtcacca ctgccataaa caacatatta
    13381 aaatatgctg ttcgccatac acccttttcg gctcgttctg gttgatttgc accaagattt
    13441 tgccccatta acgttgcagc ggcattcgaa attccccaag ccggcaaaat tgaaaaaact
    13501 aaaatgcgta ttgccgtggt ataacctgct aatgtttcct cgccaaattg cgacatgatg
    13561 cgcattaaaa aaatccaact agccgaagca ataataaatt gaccggcact acctgaagat
    13621 agtatgacta tccttctcac aatatctgtt tttaattgcc agattttccc tttcagatgc
    13681 accagtgatt ttccatttgt gaggtgatag aactgatata ttacaccaat acttctacct
    13741 attgttgttg ccactgccgc gccttgcact cccaattcag gaaatatcca tattccaaaa
    13801 atgaacaaag gatccagtac aatattgatt ccatttgaaa tgatcaaact gcgcattgcg
    13861 atgccggcat cacctgctcc acgaaaaatt ccattattta aaaacaataa aacaatggca
    13921 aaatttccgg taagcatcca tcgagtataa ccgactccat tttgtatcat ctgatcactt
    13981 gcacccatgg cgcgcaacag attttcaggg aaaaatatac caaccaatga tactgcagct
    14041 gcaatggcca atcctatgta aatagcctgc atggcggctt ctgcggctcc ttttggatta
    14101 ttttcaccta ctctcctcgc caccatcgcc gttgcgccca tactcaatcc gaacccaatt
    14161 gaatacacaa tggtaagtgt actttctgtc agtccaacaa cggcaactgc ttctacacca
    14221 actttgctga caaagaaaat atcgactacc gcaaacaaac tctccatacc catttcaaat
    14281 atcatgggta ctgcaagaag aaaaattaca gtatctaatt tccctttagt agtatctata
    14341 ttttcgccct ttagggcctg acctaataat tgaaaaaatt tacgcatgcc tatgcctttc
    14401 aaaataataa attataagaa attacctttt ccatgcagct catccaccac agtgcagaaa
    14461 gaaaaataaa ttgtggatgc taaacaatgt gttaaagtgg aagcatccta attaaaggat
    14521 catatgcaat aattgattga gcaaagataa ttgaattgga ggagaaagga gagaggagag
    14581 aggagagagg agagaggaga gagtgagtgg gtgagtgggt gagtgggtga gtgggtgagt
    14641 gggtgagtgg gtgagtggtg aatcgtgagt cgtgagtgtg gtgcgtactt gagaatcggg
    14701 tatacgatgc gaacatgcat ttgatacctg atacttgata cttgatacct gatacctgat
    14761 acttgatacc tgatac
//
LOCUS       DAOQOU010000070        14604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2106_length_14604_cov_6.261736, whole genome shotgun sequence.
ACCESSION   DAOQOU010000070 DAOQOU010000000
VERSION     DAOQOU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14604
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_14604_cov_6.261736"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /gene="prfC"
                     /locus_tag="PLM90_07595"
     CDS             <1..285
                     /gene="prfC"
                     /locus_tag="PLM90_07595"
                     /inference="COORDINATES: protein motif:HMM:NF027969.2"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HMZ89273.1"
                     /translation="VGELQFEVIQYRLEHEYGAKCRFQPIQMNKACWLTTSDKKQLDE
                     FMRLKTDYIYFDKDHNPVYMAETGWALNVAIQNHPHITFHTTSEFKTAVE"
     gene            421..1146
                     /locus_tag="PLM90_07600"
     CDS             421..1146
                     /locus_tag="PLM90_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ89274.1"
                     /translation="MRWWHFMFIFLCWQTADAQQPMRLYLLPGQGSDHRIYQNMRFPD
                     NIDTVHLHYLPVEAEETLEHYAARMVSQIDTTLPFSLMGVSLGGMICVEMSDIIHPQQ
                     VFLLSSASSGDEIPDFYHSFRKYPIYHNFNPNLIKYSTFLLQPIYEPDRRTERKTCNS
                     MIREKDADFMKSAVHMIVTWQRTEADNINTPIIQIHGTADHTLPVENIRADYYIDNGS
                     HMMTLTMADKVSAIISAAITVKQ"
     gene            complement(1155..1559)
                     /gene="arfB"
                     /locus_tag="PLM90_07605"
     CDS             complement(1155..1559)
                     /gene="arfB"
                     /locus_tag="PLM90_07605"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HMZ89275.1"
                     /translation="MPSADIISECTFKAVRSSGKGGQHVNKTSTKVELYFSIADSALL
                     QDEEKAILLTKLGHLIASDGYLRLTCEEARSQRENKILVIEKFIKLIDKALIPPKKRV
                     KTKKPKAAVEKRLTEKKHRSQIKTGRRNNSEE"
     gene            complement(1559..2008)
                     /locus_tag="PLM90_07610"
     CDS             complement(1559..2008)
                     /locus_tag="PLM90_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ89276.1"
                     /translation="MRIEEEIKQSKFNNEHHKLLLNLLYTGSWLSIKQIQFFKQFDLS
                     PQQYNILRILRGQHPAPASINLLIERMLDKMSNASRLVDKLHAKGFVDRKPCASDKRQ
                     VDVVISKKGMKLLDQIDKEIMAVDGLLSNLSKTEAKSLNELLDKIRA"
     gene            2382..2810
                     /locus_tag="PLM90_07615"
     CDS             2382..2810
                     /locus_tag="PLM90_07615"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HMZ89277.1"
                     /translation="MEFKINMVAVLVTVVVNFILGFLWYTPLFGKAWGKEMGYPADMK
                     PDPKVLMKGMIFMVIGNFLFAYVLAHNIEAWSFVPDMDQTSQFSMALSSAFFLWLGFY
                     LPGELGATVWEKHSWKLFFINTVYHFLSLLVASVILTYWT"
     gene            complement(2872..5289)
                     /locus_tag="PLM90_07620"
     CDS             complement(2872..5289)
                     /locus_tag="PLM90_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ89278.1"
                     /translation="MFCKNVAFLIAFCLLSLFTQQSLSAQSYAISGIVIDETSGLGLQ
                     DVVVTINNQQIISDKRGNFITQSNSKQLIIDCSMIGYSLINIPVIQIDTQAHKIDITI
                     KLTRNLIELPNFEITAQRVATTMPVTHTNIYASQLEQINLGRDMPFLLESVPSTVATS
                     DAGNGVGYSGLRIRGSDATRVNITINGVPYNDAESQQTYWVDLPDFASSVEDIQIQRG
                     IGITNNGLSAFGASININTNNLNKETGAFVQSSVGSFGLRKFSGGYQSGLINEHWYIQ
                     GRASIMHSDGYIDRAFADLGAWFMTGAYKGNKYTSIVNVFSGKERTYQAWGGVPAEVL
                     DTNRTYNPYTYENQTDNYRQTHVQWHQYFNVTNRDNLRLTLNYTKGSGYYEQLEEDQD
                     FGDYGIEPVLIGADTISNADLITQKWLDNDFYGAFLQYKHRHSNNWNTSTGATFYRYT
                     GTHFGKVIWNEFASTMGYDYTWYTNDAEKSDGSVYLQSDIKVGNILYLVDLQARYVGY
                     QFLGYNVEGLPVDQNVAIYSFNPKVGLSFFHGFKSKAYLFVGMSTKEPNRDDYTESTP
                     LSRPVPEKMIDVEIGDKLELDKFRISANYFMMYYIDQLVLTGEINDVGAYTRTNIPRS
                     YRTGLEVEYRYRIFPMLFLNGNVTYSINKALDYVAYIDNWDDGTQVPVAYASADLAFS
                     PEWITYNQLYGKIWDKNAASSTRYLLTATWTSKTVGQQYVDNTSDGLRSLDAYWLNDV
                     GLNMMITAKRFKEMNISLQALNVFNKAYASNAWVYTYQYGGEIQQMMGYYPQAGINWQ
                     LGFSLKL"
     regulatory      complement(5338..5439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5663..6556)
                     /locus_tag="PLM90_07625"
     CDS             complement(5663..6556)
                     /locus_tag="PLM90_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89279.1"
                     /translation="MKRIKQFTLQQYSGLAGALLLLHGNTSAQAVYTDIEPDVTLQYD
                     DDYVYIDLDGDGSNDYFLLKDSHYFSNTFYEIYRFNHVLYCGPNFNPENSIAGTFATN
                     GEGGGTTYFPYALNSGELINSDLNFQYGHYQLMAIGFYKISVTPWEWHYGIGYWTPNK
                     DSLFLGVRFLGGDDCKHYGWIRCSTADSAKTLIVHDFAYETKCETPIVAGDTIGDTTT
                     VVVNNLLELNAEIFAYNMQLFVRSNVAHQIAIFDSSGKKILDNSRQQGAYNIDMQPFP
                     KGIYIVELTTGSQMLTKIVSL"
     gene            complement(6553..8883)
                     /locus_tag="PLM90_07630"
     CDS             complement(6553..8883)
                     /locus_tag="PLM90_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89280.1"
                     /translation="MAELKPQVLRFPGGASSRWMHLLPYQDIEATFDTLDPIKGYGYN
                     IREIIRYYDGADGDIDADDSTFVNSILTDMADSVCDNCGDWMLALKYAADFENLYAKW
                     KVQQNDIPAHQQQQYIDQFIALIDSIQQWEGYTVDVIVDLNVISESASNCRHIIDYLR
                     NPAPKAEGGNGVTAVNVVGVEMGNECNLNWGTDIMGWKGFNDYWYLINGKGQADIPDG
                     YFSDDYNTWLDSLSAYVFSPEYYEDHDFISAFKSDPDLEIKVGIPAANLKNNDSTKFA
                     FRMMEDLSTNWNDTLVTHFQDSIVVGDSTRYLFDAVVLHTYYDAQTNWDSLATSNLCD
                     SLYPNSGIAGGCVTEDCDDWTNDQWQFDTIDERLSNAYKAVIGLGSNGFGNFKQFIRT
                     RYAESYDQQRLDLMLYGNEPWKKDLWTTEWNLKDKNTDYLQGSMEQNSLASFVNSFEH
                     GWLIHEWFLKNIKQNYAKNYRKGFHTYSMFHAYGGGSFYALLLQSDRADREKHLDELG
                     NPDTLTSPPANQNLFLKRTMYHTFDMLSVIKSNNLKYLPSNFTAYAHNPNIQPTVFID
                     KSNKKLYIYYSNMKTETQSYVLEKGNILMLYPGSTAMAYGEAFMYNIAPTQLYSNSGN
                     SFLYKMNICYNTTDQQHPFEIQYINGPVINEPECSGLPGGAICLTVPANSYGYIEVPF
                     YITPREGLMLSEDQIEVYPNPTANTFRIACDIPEELLNTFYVSVLDINGKLLLNTVAS
                     QNKDIDLGNLPSNIYFIVISNKEKSFSVTKKIIKAE"
     gene            complement(8884..9234)
                     /locus_tag="PLM90_07635"
     CDS             complement(8884..9234)
                     /locus_tag="PLM90_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89281.1"
                     /translation="MTFQKPGSTIICYPDTVPVELDTGTAFEIGDTIDINLAGLDTLF
                     ILYEDTNSIGDDTVSEGSEFRLVDATKDITITPGFLPKEVEHGLFGYHIEGIFTPERL
                     PQDTDNVNFPQSWR"
     gene            complement(9640..10161)
                     /locus_tag="PLM90_07640"
     CDS             complement(9640..10161)
                     /locus_tag="PLM90_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ89282.1"
                     /translation="MRKIVFTGPECTGKTTLVNKLATELQLPYVPEVARGYIDQLQRA
                     YAYDDLLNIAMLQMSEEERVKSLQPHLLICDTDLLTVKIWLDDKFQSTESWLTHAFVD
                     RTPDMYFLCAPDFPWEPDAQREDPHRREYLHKIYRDHMLHYKLRFIELNGSIEKRLYT
                     VYNYINQPLHQLF"
     gene            complement(10154..10750)
                     /gene="pnuC"
                     /locus_tag="PLM90_07645"
     CDS             complement(10154..10750)
                     /gene="pnuC"
                     /locus_tag="PLM90_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HMZ89283.1"
                     /translation="MTIPEAIAVVTGVLSVWYARKESILVYPIGIISVLLYVYICLQV
                     KLYADAAVNFYYFLASVYGWYFWLKGGRNQAESRGEEKAHIATFNMPKQIGFALLTLL
                     GGFVIGYLLDTYTDSNVPYWDGITTAIFCSAMWLMARKQIENWVYWIIGDIICVPLFF
                     SKGLCLSSLQYLIFTMLAVAGWITWNHRLKKRQEEQHA"
     gene            complement(10841..11533)
                     /locus_tag="PLM90_07650"
     CDS             complement(10841..11533)
                     /locus_tag="PLM90_07650"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMZ89284.1"
                     /translation="MKKLHLLFLLPFAATTVFISCENLNLDDLLSNDDVVAGLKEALN
                     VGTDTAVAQGSVLNGYFLNPDITILFPEEAGIVQTVVEAIPGGSLLVDEFVESMNHAA
                     EDAATKATPIFKDAILNISFDDAMSILNGADTSATNYLRVNTFSDLYTAFKPDIQNSL
                     EGVGAQQAWEDVINLYNTIPFTEDVTTDLADYTTNKGLGGLFHLVGNEEIKIRTDIEH
                     QVTDLLQQVFGE"
     gene            11714..12466
                     /locus_tag="PLM90_07655"
     CDS             11714..12466
                     /locus_tag="PLM90_07655"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89285.1"
                     /translation="MKKAFFLVSLVFTAILITESCKHTPVDLIDDLPGDPIIDTTGGG
                     PDTLVFSTCDSDTVYFENDIYPIFISNCAISGCHDAETHEEGLNLSTYTKIKTSGILN
                     LTHPWNSELIEVVTENDLDDRMPPWPMAALTDEEIQMLVTWMDQGALNNSCADCDTTA
                     ITFSGNILPIMDAYCTGCHDHTSPSGSIDLTAYNGTGSFDGISDVAADGRLVGAVTGA
                     AGYSFMPSGGMPLPDCLIEQIMTWVDEGYPDN"
     gene            12543..13235
                     /locus_tag="PLM90_07660"
     CDS             12543..13235
                     /locus_tag="PLM90_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89286.1"
                     /translation="MKSVIGLFTGGLIIAAAFGSCKHDPPFDPTQHSVTDNCDPDTVY
                     FVNDILPLIVSNCAKSGCHDGSGEEEANELTSYETIINSDYIDAYNANHSDMIEAVTS
                     GGGEDKMPPSPNQPLTSAQIDLLKTWINQGAHNNECSGGCDTTAVSYSTTIVNTMSNY
                     CNGCHSGGSPSGGIDLTTYAGVADIAGDGRLMGSIQHNAGYVAMPYNAALMPECKIDE
                     IRIWVENGFPND"
     gene            13228..13638
                     /locus_tag="PLM90_07665"
     CDS             13228..13638
                     /locus_tag="PLM90_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89287.1"
                     /translation="MTKTKKLLAFAAAVCLFTGSFLSSCYYDSMEELGYLGTVCDTTA
                     VSFSTDIQSLLDNSCLTCHSATSADALGGGNNMEGYDNLINCVTPGDPSTSSFYNSIA
                     WVSGTSFMPKAEPQLSSCEINTVMAWINQGALNN"
     gene            14084..14569
                     /locus_tag="PLM90_07670"
     CDS             14084..14569
                     /locus_tag="PLM90_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89288.1"
                     /translation="MNMIITLLKLIVTAMAKRIAIIGLLIECIDCTIRVLHVQTIREL
                     METTALTITGQAIDMHVSARQALLLELMRMQIVAIIRMAMTYTLIVFIPITNGIMSFT
                     NITITAIACIIATSMMMHVVLILSAITKSVSSFIRSGQVWLLASEPEGDNCDIHSYSL
                     F"
BASE COUNT         4329 a         3019 c         2739 g         4517 t
ORIGIN      
        1 gtcggcgaac tgcaatttga agtaattcaa tatcgcctcg aacacgaata tggtgcaaaa
       61 tgtcgttttc aacccattca aatgaataag gcttgttggc ttaccacatc tgacaagaaa
      121 caactggatg aatttatgcg cttgaaaacg gattatattt attttgacaa agaccataat
      181 ccggtataca tggccgaaac aggctgggca ttaaacgtgg ccattcaaaa tcatccgcat
      241 atcacttttc atacaacctc ggaatttaaa accgcagttg aatagcactt tgatatctga
      301 ttgtgttacc gggttgatca tccgatatta agccattgtt tcaagtttga tttcctgatc
      361 gaatacaaca agcattgttt tcggattgca attttcataa aactcctgta atttcgctta
      421 atgcgatggt ggcatttcat gttcattttt ctttgttggc aaacagctga cgcacagcaa
      481 cccatgcgct tatatctcct gcccggacaa ggttccgatc atcgcattta tcaaaatatg
      541 cgattccctg ataatataga taccgttcat ttgcactatc tccctgtaga agctgaagaa
      601 acacttgaac attatgctgc gcgaatggtc tcgcagatcg atactaccct gcccttttca
      661 ttaatgggtg tttctcttgg cggcatgatt tgcgttgaaa tgagcgacat tatacatcca
      721 caacaagtgt ttttgttgtc aagtgcatct tcaggagatg agatcccgga tttttaccac
      781 agctttagaa aatacccgat ttaccataat ttcaatccga atctgatcaa atattcaact
      841 tttctgctgc aacctatcta tgaacccgac cgccgcacag agcggaaaac atgcaatagt
      901 atgatcagag aaaaggatgc cgattttatg aaaagtgctg tgcatatgat tgttacctgg
      961 caacgcacag aggccgacaa tattaatacc cccatcattc agatccacgg cactgccgac
     1021 cacacattac ctgttgagaa catacgcgca gattattaca tcgacaatgg atcgcacatg
     1081 atgacactca caatggcaga taaagtgagc gctattattt ctgctgcaat tactgtaaag
     1141 caataatcac ttcactattc ttctgaatta ttgcggcgcc ctgtttttat ttgggagcga
     1201 tgtttctttt cggtgagacg cttttctact gcggctttgg gcttttttgt ctttacgcgt
     1261 tttttaggcg gtatcaaggc tttatctatc agcttgataa acttttcaat taccaatatt
     1321 ttattttcac gttgcgatct cgcctcctca caagtcaagc gcaaataacc atcagatgca
     1381 atcaggtgac ctaacttagt cagtaatatc gctttttctt catcttgcaa taacgccgaa
     1441 tccgctatgg aaaaatacaa ctctactttc gtagatgttt tattgacgtg ctgtccgcct
     1501 ttaccactgc tgcgaaccgc cttaaaagta cattccgata tgatatccgc agatggcatc
     1561 aggccctgat cttatcgagc aattcgttta aggattttgc ttcagttttc gacaggttag
     1621 acaacaaccc atcaacggcc atgatctcct tatcaatctg atcgagcaat ttcatccctt
     1681 tcttagagat aacaacatct acctgacgct tatcactggc acagggttta cggtctacaa
     1741 aaccttttgc atgcagtttg tcaactaacc tactagcatt actcatctta tctaacatcc
     1801 tctctatcaa taagttaata cttgccggag cggggtgttg acctctgagt atcctaagga
     1861 tattgtattg ttgcggcgaa aggtcaaatt gcttgaagaa ctgtatctgc ttgatactta
     1921 gccaacttcc ggtatacaat aagttcaaca ggagtttatg atgttcattg ttgaacttcg
     1981 actgcttgat ctcctcttca atgcgcatgt gtgatggttt gtggtttagg ttgtaagcaa
     2041 agataacaat ccatggcatt ttaacaagag acttttctct tgaaacacaa gccggataag
     2101 ggtctgatat taggaacata tgcatttatt ttgaatgcgt ttttcaataa tccgaatttc
     2161 aataccacat ttccttagaa ctactagatt tagcatacgg attaaatttt gttaatgttt
     2221 aatctgatgc tcaacaattt gtatctttag agagcagata tttaaagtta ttttgaagtc
     2281 tctgtcaggc aacatttgaa acttgtggag tccagaaacc accataataa acggggcgta
     2341 accgaaaagc caaatgagta ggcatcaaaa cttttttttc tatggaattt aaaattaaca
     2401 tggtagccgt gttggttacc gtagtcgtga acttcatttt aggcttcctt tggtacacac
     2461 cattattcgg aaaagcctgg ggaaaagaaa tgggctaccc tgccgacatg aagccggacc
     2521 caaaagtgct catgaaaggt atgattttca tggtaatcgg aaacttcctt tttgcctatg
     2581 tgctcgcgca taatatcgag gcatggagtt ttgttccgga tatggaccag acaagccaat
     2641 tctctatggc tttaagttca gcttttttcc tttggcttgg attttattta ccaggggaat
     2701 tgggcgcaac tgtatgggaa aaacattcat ggaaactgtt tttcatcaat accgtttatc
     2761 acttcctctc tttattggtt gccagtgtaa ttcttactta ctggacttaa aaataaagaa
     2821 aactaaagca cctttcattt ctaaaaaagt gaaaggtgct tttttatttt tttataattt
     2881 caatgaaaat cccaattgcc aatttatccc ggcttgtggg taataaccca tcatttgttg
     2941 tatttctcct ccatattgat aagtatacac ccatgcatta cttgcatatg ctttgttgaa
     3001 tacatttaat gcctgtaaag aaatattcat ttctttaaat cgctttgccg taatcatcat
     3061 attcaaaccc acatcattca accaatacgc atccagactt cgcagtccat ctgaagtatt
     3121 gtctacgtat tgctgaccaa cagttttcga tgtccatgta gctgttaata aatatcgtgt
     3181 tgaggacgct gcatttttat cccaaatttt tccatacaat tgattgtaag ttatccattc
     3241 aggcgaaaat gccagatcag cacttgcata tgcaacaggg acctgggtgc cgtcatccca
     3301 attatcaata taggcaacat aatctagagc cttgttgata ctgtatgtaa cattcccatt
     3361 taaaaacaac atgggaaaaa tgcgatatcg atattcaact tccagtcccg ttctgtagct
     3421 tctcggaata tttgttctgg tgtatgctcc tacatcatta atctcacctg tcaacacaag
     3481 ctgatcaata taatacatca taaaataatt agccgaaatt ctgaatttat ctagttccag
     3541 tttatcacct atctcaacat caatcatttt ttccggaaca ggtctgctta aaggcgttga
     3601 ttcagtgtaa tcatccctgt ttggctcttt tgtactcatg ccaacaaaca gataagcctt
     3661 agacttaaac ccatggaaaa atgaaagccc aaccttagga ttaaaagaat agatcgctac
     3721 attctgatca acgggcaggc cttcaacatt atatccaaga aactgatagc ccacgtatcg
     3781 cgcttgcagg tcaactaaat ataagatatt ccccaccttg atatcacttt gtaaatacac
     3841 actgccatca gacttttcgg catcattggt ataccacgta taatcatatc ccatagttga
     3901 agcaaactca ttccaaatca cttttccgaa atgcgttccg gtatatcggt aaaaagttgc
     3961 ccctgtactg gtattccaat tgttgctatg tcgatgtttg tattgtaaaa acgccccata
     4021 aaaatcgttg tccagccatt tttgtgtaat aagatcggca ttggaaatgg tgtctgcacc
     4081 aatcaataca ggttcaattc cgtaatcgcc aaaatcctga tcttcttcca actgctcata
     4141 atacccgcta ccttttgtat aattcaatgt caggcgaaga ttatctctgt tcgttacatt
     4201 aaaatactga tgccattgaa catgcgtttg tctataatta tctgtctgat tttcgtaggt
     4261 atagggatta taagttctat tggtatctaa tacttctgcc ggtacgccgc cccaggcttg
     4321 atatgtgcgt tcctttcctg aaaatacatt gacaatagaa gtgtatttat ttcctttata
     4381 agcgcctgtc atgaaccatg caccaagatc tgcaaatgct ctgtcgatat acccatcgct
     4441 atgcataatt gatgcacgac cttgaatata ccagtgctca ttgattaaac cactttgata
     4501 tcccccggaa aattttcgca atccaaaaga accaaccgac gactgcacaa atgcacctgt
     4561 ttctttattc aaattatttg tattgatatt gattgacgca ccaaatgcac tcaatccatt
     4621 attagtgatt ccgataccgc gctgaatttg aatatcctct acactcgaag caaaatccgg
     4681 cagatcaacc cagtatgttt gttgcgattc ggcatcgttg taggggacgc cattaatcgt
     4741 gatattaacc cttgtagcat cacttccgcg aatgcgcaaa ccactgtatc ctacaccgtt
     4801 tcccgcatct gaagtggcaa ctgttgatgg caccgattcc agtaaaaacg gcatgtcgcg
     4861 accaaggttt atctgttcta gttgcgaagc gtagatatta gtatgggtaa ccggcatagt
     4921 tgtagccaca cgttgtgccg tgatctcgaa attaggcagc tctattaagt tcctggtaag
     4981 ctttatggtg atatcaattt tatgcgcctg ggtgtcgatt tgaataacag gaatattaat
     5041 taacgaatat ccaatcattg agcagtcaat tatcaattgc ttactattcg attgagtaat
     5101 aaaattcccg cgcttatctg atataatctg ttggttgttt attgtaacta caacatcctg
     5161 caagcctagt ccgcttgttt catctattac aattccggat atcgcatacg attgagccga
     5221 gagcgattgt tgtgtaaaca aactcagcaa acaaaaagca attaaaaaag ctacgttttt
     5281 acaaaacatg gcaaaggttt acattatgaa tcctgtaaat ggagccttta ccggtaagaa
     5341 tactaatttc ctctatccct tcccggcatt acccgggcag gttcgatggg tttaatctca
     5401 gtcgttcaga aaatctgtcg gacaccccat gagatatgca aagatactag aattcattta
     5461 ataataccat tggcatcttt cctgcgttga tgcctactac cgtcctataa ataagtggcc
     5521 cttataactt aggttagact agcttactgc aagcatagtg ggacgggttg cttaattgcc
     5581 ttgaataaaa tgggtatact tgaaacctat aactctaaca tatttctgag atggaatcgg
     5641 attctatcaa taacatacct cgtcataaag atacgatctt agtcagcatt tgcgatccgg
     5701 ttgtcaattc tacgatgtaa attccttttg ggaaaggttg catgtcgata ttgtatgcgc
     5761 cttgttgccg actgttatca agtattttct tgccgctgct gtcaaatatt gctatttgat
     5821 gggcgacatt acttctaaca aacaattgca tgttgtaagc aaatatttct gcatttaatt
     5881 ccaataaatt gttcacaaca actgtagtag tgtcgccaat ggtatcgccg gcaacaattg
     5941 gcgtttcgca ttttgtttca taagcaaaat cgtgcacaat caatgtcttg gcagaatcag
     6001 ctgttgagca cctaatccat ccataatgtt tgcaatcgtc accgccgaga aaacggacgc
     6061 ctagaaacag tgagtcttta tttggtgtcc aatagcctat cccataatgc cattcccaag
     6121 gtgttacact aattttataa aatccaattg ccatcaactg ataatgtcca tattgaaaat
     6181 tcagatcact attgattaat tctccggagt taagggcata ggggaaatag gtagttcctc
     6241 cgccttcacc gttggtggca aatgtacccg ctatgctatt ttcaggatta aaatttggac
     6301 cgcaatatag gacatgatta aatctataaa tttcataaaa tgtattagaa aagtaatgac
     6361 tatcttttaa taaaaaataa tcattgcttc catcgccatc caggtcaata tatacataat
     6421 catcatcata ttgcaatgta acatccggtt ctatatctgt ataaactgct tgtgccgaag
     6481 tgttaccatg caataataac aatgctcctg ctagaccgga atattgttgc aaagtgaatt
     6541 gttttatacg tttcattcag ctttgatgat tttttttgtt acagaaaagc ttttttcttt
     6601 attgctaata acaataaaat agatatttga aggtaggttg cctaagtcaa tatctttatt
     6661 ttgagaggct acagtattta ataatagttt gccgtttata tctaaaactg atacatagaa
     6721 tgtgttgagc aattcttcag gtatatcaca tgcaattcga aaggtatttg cagttggatt
     6781 gggatacacc tcaatttgat cctctgacag catgagccct tctcgcggag tgatataaaa
     6841 tggcacttca atataaccat aagaatttgc agggactgta aggcaaatgg cacctcccgg
     6901 taaacctgag cattcaggtt cattgataac tgggccattg atgtattgga tctcgaacgg
     6961 atgctgttga tccgtagtat tgtagcagat attcatttta tataaaaaac tattaccgct
     7021 attggaatag agctgtgtag gagcaatatt atacataaat gcctcaccat aggccatagc
     7081 ggtagaaccc ggatacagca tcaagatatt acctttttcc aaaacatagc tttgtgtttc
     7141 ggttttcata ttggaatagt aaatatacag ctttttattt gatttgtcaa taaaaacagt
     7201 tggttgtata ttgggattat gcgcataagc ggtaaaattc gaaggcaggt acttaagatt
     7261 gttacttttt attacactga gcatatcaaa tgtatggtac attgtacgtt tcaaaaataa
     7321 attttgattg gcaggcggtg aagttaaagt gtccggattg ccgagttcat caagatgttt
     7381 ttctcgatct gccctgtctg attgtaaaag caaggcgtaa aatgaccctc caccataagc
     7441 gtgaaacatt gaataagtat gaaagccttt tcgatagttc ttagcatagt tttgtttaat
     7501 gtttttcagg aaccactcat gaataagcca gccatgctca aatgagttaa caaatgaagc
     7561 tagtgagttt tgctccattg agccttgtag gtaatcagta tttttatctt taaggttcca
     7621 ttctgttgtc cataaatctt ttttccacgg ttcattccca taaagcatca agtcgagcct
     7681 ctgttgatcg taagattcag cgtaccttgt tcgaataaac tgtttaaagt tcccgaatcc
     7741 atttgaaccc aatcctatta cagctttata agcattgctt aggcgttcgt caattgtatc
     7801 gaattgccac tgatcattag tccaatcgtc acaatcttct gtaacacatc caccggctat
     7861 gcctgaattt ggatatagag aatcacataa gttactagtt gccaatgaat cccaattggt
     7921 ttgagcatcg tagtaggtgt gtaggacaac cgcgtcaaat aaataacggg ttgaatcacc
     7981 aacaactata ctgtcttgaa aatgcgtcac caaggtatcg ttccaatttg ttgacaaatc
     8041 ttccatcatg cggaaagcaa atttggttga gtcattattt ttgagattgg ccgccgggat
     8101 tccaaccttt atttctaaat ccgggtcaga tttgaatgct gaaatgaaat cgtgatcttc
     8161 ataatattca gggctaaata catatgcgga aagggaatcg agccaagtat tgtaatcgtc
     8221 tgagaagtaa ccatcaggga tgtcagcttg accttttccg ttaattaagt accaataatc
     8281 attaaaaccc ttccagccca taatgtccgt accccagttc aaattgcatt catttcccat
     8341 ttcgacgccg accacattta cagctgttac accgtttcct ccttctgctt ttggtgcagg
     8401 gtttcgaagg taatcaataa tatgcctgca attagatgca gattcactaa taacatttag
     8461 atcaacaatt acatctactg tgtatccttc ccattgctga attgaatcaa ttaaagcaat
     8521 aaattgatca atatattgct gttgttgatg tgcagggatg tcattctgtt gaaccttcca
     8581 tttcgcgtac aaattttcaa aatctgcagc atatttcaat gcgagcatcc aatcaccaca
     8641 attgtcacaa acagaatctg ccatatctgt gaggattgaa ttcacaaatg tagaatcatc
     8701 tgcatcaata tcgccatctg ctccatcgta atatctaata atttctctaa tattatatcc
     8761 ataaccttta ataggatcaa gcgtatcaaa tgtggcttct atatcttggt aaggtaataa
     8821 atgcatccaa cggctacttg ctccgcccgg aaaccgcaag acttgtggtt ttaactctgc
     8881 cattcatctc cacgattgtg gaaaatttac attatcggta tcctgcggta aacgttcagg
     8941 tgtgaaaatt ccctcaatat ggtacccgaa tagcccatgt tctacttcct ttggtaaaaa
     9001 accaggggtt attgtgatat cctttgtcgc atctactaat ctgaattcgc ttccttcaga
     9061 aacggtatcg tcacctatag aatttgtgtc ttcataaagt atgaataatg tatctaatcc
     9121 tgctaaatta atgtcaatgg tatctcctat ttcaaaagct gtaccggtat cgagttcgac
     9181 aggaacagta tccggataac aaattattgt acttccgggt ttttgaaaag tcaattcatt
     9241 tttaaggtgt aattctgtgt tcttgacaat tgcactaaat aaatttgaac caatgaaagt
     9301 caaaagtact atgtaaattg caatgggatt ttgttgaatt tgatttatta gttttcataa
     9361 ggtatatttt aataagatga tgagtgaaat taaagaaata attaaagaaa atacatttga
     9421 tttacatcat ggattttttt gataaattca gctaaacccg taagtaatca attaaatctt
     9481 tacaaggtac aaaatagtcc ccattttctc acacttttat aatatgttac catcgcacca
     9541 cttcgaaagc ttgaattctg ctataataac caattaatca aagcgtcaat ttacccacct
     9601 aaattcaaca atagtaattc tgccaataaa cgacacattt taaaacaact gatgcaaagg
     9661 ttggttgata taattataga cggtatacaa acgcttctct atagagccgt tgagttcaat
     9721 gaaacgcaat ttgtagtgca acatatggtc gcgatatatt ttatgcaaat attctctgcg
     9781 atgtggatcc tcacgctgcg catccggttc ccagggaaaa tccggcgcac ataaaaaata
     9841 catatcagga gttctgtcta caaaagcatg tgtcaaccat gattctgtgc tttgaaattt
     9901 atcgtcgagc caaattttta cggtgagtaa gtcggtatcg caaatcaaca gatgtggttg
     9961 caaagatttt actctttctt cctcactcat ctgcaacatg gctatattga gcagatcatc
    10021 ataggcatat gcacgttgca gctgatcgat atatccacgc gccacttcag gcacataggg
    10081 caattgtagt tctgttgcta atttattgac cagagtagtt ttacccgtgc attcaggtcc
    10141 ggtaaatacg atcttacgca tgctgctctt cctgccgctt tttcaggcga tggttccaag
    10201 ttatccatcc ggccactgcg agcattgtaa atataagata ttgcagtgaa ctcagacaca
    10261 atcccttaga aaaaaataat ggcacacaaa taatatcgcc gatgatccag tatacccagt
    10321 tttcaatttg cttcctcgcc atcagccaca tcgcagagca gaaaattgct gtggtaattc
    10381 catcccaata aggcacatta gaatcggtat atgtatccag taggtagccg attacaaatc
    10441 caccgagaag tgtaaggagt gcaaaaccaa tttgtttcgg catgttgaat gttgcgatat
    10501 gcgctttttc ttctcccctg ctctcagctt gattacgccc tcccttcagc cagaagtacc
    10561 atccgtaaac acttgccagg aaatagtaaa aattaactgc ggcatcggca tacagtttta
    10621 cctgcaaaca aatgtatacg tacaacagca cactgataat accgatagga tacaccaaaa
    10681 tcgactcttt tctggcatac cagacactca acacccctgt gaccaccgcg atcgcttcgg
    10741 gtattgtcat caacaatacg gaatcatgta aacaatggct taactgcacc ttgaaatttg
    10801 tgcaaatcta cctattcaaa gaatgatagt ttaccacaaa ttattcccca aacacctgct
    10861 gcaacaaatc tgttacctga tgttcaatat cggtacggat tttaatctct tcgttgccta
    10921 ccagatggaa taagccaccc aaacctttat tggtagtata atctgcaaga tcagtagtaa
    10981 catcctcggt aaacggaata gtattgtaaa ggttgattac atcctcccaa gcctgctgcg
    11041 caccaacgcc ttccaaggaa ttttgaatat caggtttgaa agcggtatag agatcagaga
    11101 atgtattgac tcttaaatag tttgtagcag aagtgtcagc gccattgagg atagacatgg
    11161 cgtcatcgaa ggaaatattc agaatggcat ctttaaaaat cggggtggcc ttggtagcgg
    11221 cgtcttcagc ggcatgattc attgattcta caaattcatc aaccagcaaa gagcctccgg
    11281 ggattgcttc aaccactgtt tgcactatgc ccgcttcctc aggaaacaga atggtaatat
    11341 ccggattaag aaaataacca ttcagcaccg aaccctgcgc caccgctgta tctgtgccca
    11401 cattcaaagc ctctttcaat cccgctacca cgtcgtcatt ggatagcaga tcatcaagat
    11461 tcaggttctc gcaagagatg aaaacagttg ttgcggcgaa aggcaacagg aagagaagat
    11521 gcagtttttt cataaaatgg ctgatttacc gatacgaaat taaaaaatcg tgccagtttt
    11581 ccccaaatgt gggcgaaaaa ggcaactatt caagagaaaa aaggtcaaaa aagtcggaat
    11641 tgtgtgataa atgtccatga ggcacccttt ttgcatagtt aattttgtaa tatattatta
    11701 ctactctgat attatgaaga aagcattttt ccttgtatcc ctagtgttca cggcaattct
    11761 gatcactgaa tcatgtaagc acacccctgt agatttgatt gatgatctgc ccggtgaccc
    11821 tattatagat accacgggag gggggcctga tacccttgtt ttcagcacgt gcgactcgga
    11881 taccgtgtat tttgagaacg atatctaccc tattttcata tcaaattgtg cgataagtgg
    11941 ctgtcacgat gcggaaacac atgaagaagg tcttaacctg agcacttaca ccaaaatcaa
    12001 aaccagcggt attcttaacc tcacgcatcc ttggaatagt gaacttattg aagttgtcac
    12061 cgaaaatgat cttgacgacc gcatgccgcc ttggcctatg gctgccttga cagatgaaga
    12121 aattcagatg ttggttactt ggatggatca gggtgctttg aataatagtt gtgcagattg
    12181 cgatacaact gccataacat tctccggcaa tattttaccc atcatggatg cctattgcac
    12241 cggatgtcac gaccatacca gtccttcggg aagcatagat cttaccgcct ataacggaac
    12301 aggaagtttt gatggcattt cagatgtagc tgctgacggc agacttgtag gtgccgtaac
    12361 aggagctgca ggttattcat tcatgccttc cggcggtatg ccattgcctg attgtttgat
    12421 tgagcagatc atgacctggg tggatgaagg ctatcctgat aattgatgag taaaatcatc
    12481 attttcaagg aatgaatggt acgcagttga tgttgtaata agcagaaaag agattgaatt
    12541 gtatgaaaag cgtaattggg ttatttaccg gcgggttgat tattgctgct gctttcggtt
    12601 cctgtaaaca cgatccacca tttgatccga cacaacattc cgtgactgat aattgtgatc
    12661 ccgacaccgt ttatttcgtg aatgatattt taccactcat cgtcagcaat tgtgcgaaaa
    12721 gtggttgtca cgatggcagt ggcgaagaag aagccaacga gttaacctcg tatgaaacta
    12781 ttatcaatag cgattatatt gatgcctaca atgccaatca tagtgatatg atagaggcag
    12841 taacttcagg aggcggtgaa gataaaatgc caccaagccc gaatcaacca ttaacttcgg
    12901 cccaaattga tttactgaaa acctggatca accagggagc acataacaat gaatgttccg
    12961 gtggttgtga cacaacagca gtaagctaca gcaccacaat tgtaaatacc atgtcgaact
    13021 attgcaacgg ttgtcacagc ggaggaagcc cttccggtgg tatagacctc actacttatg
    13081 caggtgttgc cgatatagca ggtgatggaa gattaatggg aagtatacag cataatgcgg
    13141 gatatgtggc catgccatat aatgccgccc taatgcctga atgtaaaatt gatgaaataa
    13201 gaatctgggt tgaaaatgga tttccaaatg actaagacta aaaaattact ggcctttgcg
    13261 gctgcagttt gcctttttac aggcagtttc ctcagttcat gttattacga tagcatggaa
    13321 gaattaggtt acctcggaac agtatgcgat actacagcag tatcgttcag caccgatatt
    13381 caatcgctgc tcgacaactc atgtcttacc tgccacagcg ccaccagtgc cgatgcattg
    13441 ggtggaggta acaatatgga aggatatgac aatctgatca attgcgtaac gcccggtgac
    13501 ccatctacaa gttcatttta taattctatt gcctgggtat caggaacaag ttttatgccc
    13561 aaggctgaac cacaattatc ttcttgcgag ataaataccg ttatggcatg gataaaccag
    13621 ggtgcattaa ataattaagg tatttaaata ttggtgagta tcgatctcac cggcgactca
    13681 ggagcagtga ttttattatt actgctcctt ttgtttttgc atttggcaaa ttatggttat
    13741 taaatttatt ttttgtttta tatctttgcg acagcaatga gtaagaacat tatcaattgt
    13801 agataaaaac cgaatccgta aaaatatttt tacccggacc ggttccgggt atttttttgc
    13861 ccatacatga aggtgtttta ctatttattc ataaaaacaa attatatgca acatgttgaa
    13921 tttccgtccg atgatagcat tacgctaaaa cgtagtggct atgcgtgttg cacctgatca
    13981 taacattcat caagatcggc ccggcaacac tatgtccggg cttttttgtg tccggaaatg
    14041 atgtattttt tttcacgcgg cattccgcac aaatccgatc attatgaaca tgatcattac
    14101 cttattaaaa ttaattgtta ccgctatggc aaaaagaatt gccatcatcg gtttgttgat
    14161 tgaatgcata gactgcacca tacgtgtatt gcatgtacaa accattcgcg aattaatgga
    14221 aacaactgcg ttgaccatca ccggtcaggc aattgacatg catgtgagtg cacgtcaggc
    14281 tttattattg gaattgatgc gtatgcaaat tgtcgccatc attcgaatgg caatgacata
    14341 tactttaatt gtattcatac caattacaaa tggtataatg tctttcacca atattaccat
    14401 aacagcgatt gcctgcatca ttgctacaag catgatgatg catgttgttt tgattttgtc
    14461 tgcaatcacg aaaagcgtca gttcattcat tcgatccggc caagtgtggt tgttggcgtc
    14521 ggaaccggag ggagataact gtgatataca cagttattcc cttttttgag atatgtacaa
    14581 taccgcaaag acagtacatt tgcg
//
LOCUS       DAOQOU010000071        14514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2130_length_14514_cov_5.419739, whole genome shotgun sequence.
ACCESSION   DAOQOU010000071 DAOQOU010000000
VERSION     DAOQOU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14514
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2130_length_14514_cov_5.419739"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLM90_07675"
     CDS             <1..706
                     /locus_tag="PLM90_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89289.1"
                     /translation="ALGGLFATTIRAAIILIGAGETITNMSAYLADMGFQNFSVFNRT
                     LANAEKLVSSIGGKAYQLDALKTFTGGFDVIISCTGSVEPVINEEIYQQLIGRDTQKK
                     VIIDLAIPQDIDTTILSRNQIDYIHIDDLRSIAQKNLSLRKAEVVKAKKLVHEFVKEF
                     QAAYIERLVEKAHSAIPQKLHEIRLRAVEEVYAKDVASLDPHAKEVMDKLLDYMEKKY
                     LALTMASSKSNLRHPH"
     gene            706..2259
                     /gene="hemC"
                     /locus_tag="PLM90_07680"
     CDS             706..2259
                     /gene="hemC"
                     /locus_tag="PLM90_07680"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HMZ89290.1"
                     /translation="MKHKVIIGSRGSDLALWQANYLKKSLQEIGVDADILIIKTQGDQ
                     IQHVSLEKLEGKGFFTKEIEDALLENKIDIAVHSHKDLPTATTPGLKIAAVSYREDPS
                     ELLLVRKEIADKTQFWNLPLHAKVGTSSSRRAVQLHFYRPDIQLADIRGNVPTRVQKL
                     RNGQYDAILLAAAGVQRLQLLLDDLLAIKISPHHFIPAPAQGVLAYQCREQDLEILEI
                     LEKLHHQSVYDTISVERKVMQLFNGGCHMPLGVYCQYMHGEFFVWATQSEDKLNTPRR
                     LFVRSKNNHNLAEVIYDKLRNRSDKKVLFTTEPEAAEQAIKIVEAAGHSVIAQPYIKI
                     VPKYFDFNIDCDWLFFTSKNGVNEFFKVVKQMPSSIKIAAFGAETAEKITTFGYDVAF
                     TGNGLVEDVAKQFYEHGKTMHVVFPCAVNARKELHEAIAPYAHVFALPVYENILISNE
                     IFEADIIACSSPMQAEGYLKQHTPDGKTFIAIGPTTARYLENNGISNVHVAPFPSLIS
                     MAELICGLV"
     gene            2295..3401
                     /locus_tag="PLM90_07685"
     CDS             2295..3401
                     /locus_tag="PLM90_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ89291.1"
                     /translation="MAFGINSRRIKKAIRRLKSLPRGANFFRYTSNKVYGAYLRMIHS
                     TKVAHPSSIMLEVTNHCNLKCITCPREYRYGEEMDKGFMDIAQLKKVIDQAYPYIDSV
                     GLTGLGETLLYKQLPEALAYIRSKNKGIITTISINAHLPNSVELVEKIADDLDTLQIS
                     MDGVGEVYEQVRLRGEFSLFYDNVKRIVELCKNRRADVMFNFVAIKENYFNMAEVVEV
                     ASELGVDYINVTPFNVAAVTAHDISYYDFFHSDAFKNELKRAKDKADEVKNVTLTIWD
                     IKTPNTYQKCHLPWSHFYISWDGYATPCCAKPFPKELNFGNIFDDGLMYCLNTPGYRE
                     FRRLWYENKTPQFCEKCHMVDLTPIDLPFELPVN"
     gene            complement(3398..3613)
                     /locus_tag="PLM90_07690"
     CDS             complement(3398..3613)
                     /locus_tag="PLM90_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89292.1"
                     /translation="MTASTFTLLIDILTLLFMIVGALWMWGVFKFKAEVQQRFEEIFA
                     RSRKRFKWMFLIGAILLAALFVRDYFL"
     gene            3692..4543
                     /locus_tag="PLM90_07695"
     CDS             3692..4543
                     /locus_tag="PLM90_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89293.1"
                     /translation="MFLTVLSCQAQIWTLEKNGVQASASYTWFHYDKAYQDNSTTIDL
                     PLTVFDKTLALDVQYGITDRITALARLPYKMLETTGYPGVYNSYPGQYIETGKLNNIG
                     NTEFGLVWKCYDDKPIVSASFIVEANTSDRNYITGLQTGFNSWAFRPGIGVGWGFDKS
                     WLQFYTGMSIRTNDYALAVVSNLEAGYHFADYWWGAISLDTRKPNVDGEDCDCSTSAT
                     MLYQNAQEYYALALKTGFTMYKDWGLNFGYHLGLTGNDVAATAVPVIGVMYKSRPMRH
                     TIITPRF"
     gene            complement(4547..5164)
                     /gene="coaE"
                     /locus_tag="PLM90_07700"
     CDS             complement(4547..5164)
                     /gene="coaE"
                     /locus_tag="PLM90_07700"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793821.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMZ89294.1"
                     /translation="MIKVGITGGIGSGKSTVCKIFEVLGVPVYYADERAKEILQTDAE
                     IIKRVKDLFGEDVYDSNHVLDRKRVAFVVFHEPEFLAQYNKIIHPAVLLDSEKWMQRH
                     LQYPYVIKEAALLFESGGNKQLDKVICVTAPEDLRIQRVMQRDNTTEEQVRSRMANQM
                     SEEEKLEKSDFILYNDGSAPLIRQVLNIHEKLLEIIRKQDQLEKR"
     gene            complement(5166..6071)
                     /locus_tag="PLM90_07705"
     CDS             complement(5166..6071)
                     /locus_tag="PLM90_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89295.1"
                     /translation="METMGDSTPAAQRSFAGEFALFFVCLFIAVIIWLFTALSENYEQ
                     EINVGLSYVLPESQTTTIPLPQEAHLLVSSSGWQLVKEGFRHRAMSLDVSDLDDKPVL
                     LTNNHLGLFTAGLPGNMHIVQVIPDTIDLHLEPLAERKVPIVLNADFMDRTDIVVDSI
                     YIHPDSITIRGPKSLITDITYWSTEAIAGNGDSIIDGITPLPFPAVGSIRLSAILVDW
                     HAEIEAAIEANAYLTINTPTNHAVDVRIQYSSAAEVSSLISAKDFNITFEKTADGTQY
                     HIVARAKRKGLHTIGVYPAFIPITR"
     gene            complement(6078..6392)
                     /gene="yajC"
                     /locus_tag="PLM90_07710"
     CDS             complement(6078..6392)
                     /gene="yajC"
                     /locus_tag="PLM90_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMZ89296.1"
                     /translation="MAPAADGQSSGSSWVQIAFFFLFFLILYFFMIRPQSKKAKEQKN
                     FLNEIKVGDKIVTIGGVHAKILKIDDDTYLIEVDTNTKLRIEKSVISLDFTRAMLARK
                     QG"
     gene            complement(6438..6899)
                     /locus_tag="PLM90_07715"
     CDS             complement(6438..6899)
                     /locus_tag="PLM90_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ89297.1"
                     /translation="MKKQVLFASFALIFGAGILLSCKEQSPVDVKAKSGSDEKLNADL
                     VKNQTSITFERDMHDFGEIIKGEKVSTEFKFMNTGKNDLLISDAHGSCGCTVPEWPKE
                     PIPPGGSGVIKVVFNSGNRSGEFNKTVTVIANTVTNPNKLIIKGTIITPTN"
     gene            complement(6915..7853)
                     /gene="nusB"
                     /locus_tag="PLM90_07720"
     CDS             complement(6915..7853)
                     /gene="nusB"
                     /locus_tag="PLM90_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HMZ89298.1"
                     /translation="MLSRRGLRIKAMQTLYVAACSGQPEPKAALSHLSKSIRNSHLAY
                     LYILHFLTELAQQVDLEAHIKKNKYLPSAEDLAFPTTFFDNKVIQGLLKNNYFQSRLK
                     REKLTDLVDEEYSKIIYQQLKEYAPYNNYVGSSPDLVADKKMLKDIFEHFLLKNEYFD
                     EHMENSIATWADDEFIVKGLMDEFFNNNISEQTEKHLFDFQVGDEEMAFAESLVVFTM
                     ESDLQYDEEIKPKLQNWELDRVSLIDRILMKMALTEFLHLPTVPTKVTINEYLDISKL
                     YSTPRSREFINGILDKLMNEMKSSGRIVKTGRGLIE"
     gene            complement(7901..8998)
                     /locus_tag="PLM90_07725"
     CDS             complement(7901..8998)
                     /locus_tag="PLM90_07725"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMZ89299.1"
                     /translation="MSNNTLQLTNGEVFTTMREMEHEQLVFCTDNNTGLKAIVAIHNT
                     TLGPALGGTRFWSYQQEDDAIIDVLRLSRGMTYKAAISGLNLGGGKAVIIGDSKALTN
                     REPLFRRYGKFVNGLAGRYITAEDVGTSTSDMEFINMETDHVAGKPEYLGGGGDPSPV
                     TAYGVYLGMKSATKYATGSDNLSGKRILVQGVGHVGYNLVKHLTKDGAKVMISDISEE
                     RLKAVAAEFGAEIIGTDKVFDTQMDIYAPCALGATINDDTINKLSCQIIAGAANNQLK
                     DEAVHGKRLIERGITYAPDYLINAGGLINCYVELEGYNRERALSKTEIIYDRAMEIFQ
                     VAAKNNITTQAAANQVAEERIRAIANINARL"
     gene            9108..10898
                     /locus_tag="PLM90_07730"
     CDS             9108..10898
                     /locus_tag="PLM90_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ89300.1"
                     /translation="MNALRPVHQYLLKYKWLFLTGTLFVGLANLFQVYAPHFIGDMID
                     AVAENADTVINGTDSEKASAVYRFILINALLFIGITLLRGVVMFFMRQTIIVMSRKIE
                     YDQKNELFRKYEFLTPAFFKRNNTGDLMSRVAEDVGRVRQYVGPAIMYFLNLLFNFIF
                     GIWMMLSINPTLTLYVLLPLPILSVSIYFVNSIINKKSEQIQAQLSHLTSLAQESFSG
                     IRVIQAYAREKQTVNFFADACDDYKTDSLSLARIDAMFQPLMVLLIGMSILLTIFVGG
                     WQVIQGNLSPGNLAEFLFYVNMMTWPVTSLGWVVSIIQRAAASQKRISEFLDLQPDIV
                     SEGLAPKQVKGRITFKNVHFTYPDSGVKALDGVSFDIGIGETVAIVGRTGSGKSTIAD
                     LIFRLYDANDGSILIDGEPITSFNLTDLRNGVSMVPQDVFLFSDSIAHNIAFAAPDAD
                     VERVKQFAQFASVDKEIESFKSGYDTIVGERGVTLSGGQKQRVSIARAFMKESPVIIL
                     DDCLSAVDAGTEHIILGNMRRYLEKRTAIVITHRITSLLEFDKIIVMDQHQVAEYGTH
                     DELMALNGLYAKMVNAQRDVTTSELKNNVR"
     gene            10976..11416
                     /locus_tag="PLM90_07735"
     CDS             10976..11416
                     /locus_tag="PLM90_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HMZ89301.1"
                     /translation="MEHENSNRNDSVFSRKIRAGRRRTYFFDVRTTKGNDYYITITES
                     KKRFDGNGYDRHKLFIYKEDFNKFADELQQVIDHIKGLLPDFDFDAFAHRYDENGEHY
                     DGGNHQHHDDDREQYNDNSNSSSSDNTSTDNNSGHHYGDEDLKL"
     gene            11535..12785
                     /locus_tag="PLM90_07740"
     CDS             11535..12785
                     /locus_tag="PLM90_07740"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89302.1"
                     /translation="MSKWCSRSMQFLVVILTFLSLDSKGQNLVPNNGFETNDGTPTGY
                     GQWYHATGWSDVNNYPAFLWPYASPDYLHNTGAGGADLPVCTFGTVSPFAGNAVMGFV
                     TWLQSTPDFREYLSIPLSSPMIVGTAYNVSFWITNGSASWYNGYSSNRIGARFSIGTL
                     TQATHEPIGGTPQCELVTDLWTTTWQYVSFVYTPDQAYNQITIGNFYNDASTSHSYHV
                     ASLSAGAYYFIDEVVVEPAVPLPVELLAFEAVQTDHGADISWNTASEFDVSSYVVSRS
                     SDGVNFNPLGEISATGNATTSADYLLLDDEPLFGNNYYQLEVVNLNGEVDETYLTALY
                     VDGTASRPVVYPNPSRGSVTISIPIGLPLAGIQIFDISGRLIREINTLSATVPIGIND
                     LSTGTYVIKFISTIDIDNQLLIFE"
     gene            complement(12804..14399)
                     /locus_tag="PLM90_07745"
     CDS             complement(12804..14399)
                     /locus_tag="PLM90_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89303.1"
                     /translation="MKQLGNISAYLLIITILIPICEVRGQNTNISDGYLFDGEPFITI
                     NPSNGSHMVVAWMGVNGLDLVQIMTRVSTDGGNSWSAEYAQPHIAGGYTSADPSMDWD
                     SDGNLYLCYIDYSPFGTSGKTIVRKSADGGFTWGDPVEVIDAFDDPGEVPVDRPWLVI
                     DRSGGLDDGFQYVTTKPAPWIPFPNRNYLTVSKNGGLSFEDWRYIDTTGWQIGDFIQA
                     PMAAPAVGPDGTLHCVYPAWEPTENLLPRFIHASSVNGGDGFSYYEMLESFGGDLNAD
                     TSAKTGYQLLVDPTDPHHLVFLFVFRPLGDNDIYCLESANSGVNWSTPLRINADPAGN
                     KVMQDLVWGDFDGEGNLVVTWRDRRNAVDTGYAVASEIWGAVRYKDSIAFSNDFRISS
                     ALAAFDSVLYGNGNDFMGVQLFNDTLYAVWGDTRNDYLNIWFSKQAVGSSTPTGVINL
                     GSEQLFEITAYPNPAGNYLMVEGDNISRLQIRTASGQVYLDQSITTTGDIRLSLENIP
                     AGNYFLAAFIGNDVAVRQIAITK"
BASE COUNT         4191 a         3193 c         2929 g         4201 t
ORIGIN      
        1 ggccttgggt ggtttgtttg cgacgacgat tcgtgccgca attattttga ttggggcagg
       61 ggagacaatt actaatatgt cggcctacct tgctgatatg ggttttcaaa atttctcagt
      121 gtttaatcgc acattggcaa atgcagaaaa actggtctct tcaatcggtg gtaaagctta
      181 tcaattagat gccctgaaaa catttaccgg tggatttgat gtaatcatca gttgcaccgg
      241 atctgtagag cctgtaataa atgaggagat ctatcaacaa ttgattggtc gcgatacaca
      301 aaagaaagtg attatcgatc ttgctattcc gcaggatatc gacacaacta ttttatcgcg
      361 taatcaaata gattacatcc atatcgatga tctccgcagc attgcgcaaa aaaacctcag
      421 cctgcgcaaa gccgaagtag taaaggctaa aaaattagtg catgagtttg taaaggaatt
      481 tcaagccgca tatatcgagc gcctggttga aaaagcacac agcgcaatac cacaaaaatt
      541 acatgaaatt cggttaagag cggtggaaga agtgtatgca aaagatgttg catccctcga
      601 tccgcatgca aaggaagtaa tggataaatt actcgattat atggagaaaa agtatctggc
      661 tcttactatg gcttcatcta aaagcaattt gcgccaccct cactgatgaa acataaagtg
      721 atcatcggtt cacgaggaag tgatctcgca ttatggcagg caaattatct caaaaaatct
      781 ttgcaggaaa taggtgttga tgccgatatc cttatcatca aaacgcaagg tgatcaaatt
      841 cagcatgtca gtctcgagaa attggaggga aaaggttttt ttaccaagga aattgaagac
      901 gcgcttcttg aaaataaaat tgatattgcc gttcattctc ataaggacct gccaacagca
      961 actactccgg gactcaaaat agctgctgtt tcttaccggg aagacccttc ggaattatta
     1021 ttggtgcgca aggaaattgc cgacaaaaca caattttgga atttgccgct gcatgcaaaa
     1081 gtcggcactt cttcatcaag aagagctgtt caattgcact tctatcgtcc ggatattcaa
     1141 ctggctgaca tcagaggtaa tgtgccaact cgtgtgcaga aattgcgaaa cggacagtat
     1201 gatgctatat tgcttgcggc agcaggtgta cagcgattac aattgctact cgatgattta
     1261 ttggcgataa aaatttcacc acatcatttc attccggctc ccgcacaagg tgtgttggca
     1321 tatcagtgca gagaacagga tctcgaaatt ttggaaatac tcgaaaaatt acatcaccaa
     1381 agcgtatacg ataccatcag tgttgaacgc aaagtaatgc aattgttcaa tggaggctgt
     1441 catatgccct tgggcgttta ttgccaatat atgcatggcg aattttttgt ttgggcaacg
     1501 caatccgagg ataaattaaa tacgcctcga agattatttg tgcgcagcaa gaacaaccac
     1561 aatcttgctg aggttattta tgataaactg cgcaaccggt cagacaaaaa ggttttattt
     1621 acaacagaac cggaggcagc agaacaggca ataaagattg ttgaagcggc aggacattca
     1681 gtgattgcgc aaccatatat taaaatagtg ccaaaatatt tcgattttaa tatcgattgc
     1741 gattggctgt ttttcaccag caaaaatggg gtgaatgaat ttttcaaggt ggtaaagcaa
     1801 atgccatcat ccataaaaat tgcagccttt ggtgcagaaa cagcagaaaa aataacaaca
     1861 tttggatatg atgttgcatt taccggaaac ggattagtag aagatgttgc caaacaattc
     1921 tatgagcatg gaaaaacaat gcatgtggta tttccatgtg cggtgaatgc ccgaaaggaa
     1981 ctacatgagg caatagcccc atatgctcat gtttttgcct tgccggttta tgaaaatatt
     2041 ttgatcagca atgaaatctt tgaagctgat attatcgcct gctcaagccc aatgcaagcc
     2101 gaagggtatt taaaacagca tactcctgat ggcaagacat ttatagccat cgggccaaca
     2161 acagcacgat atcttgaaaa taatggtatt tcaaatgttc atgtagcacc cttcccgtca
     2221 ttgatcagca tggctgaact gatctgtggt ttagtataga tatcggtctc aacagttatt
     2281 tgtaaatttg tgccatggcg tttggcataa atagcaggag gataaaaaag gcaattcgca
     2341 gacttaaaag tttgccgcga ggagccaatt ttttccgcta tacttccaat aaagtatatg
     2401 gcgcctacct tcgcatgatc cacagcacca aagtggcgca tccgtcgtct atcatgctgg
     2461 aggttaccaa tcactgtaat ttgaaatgta ttacctgtcc aagagagtac cgctatggtg
     2521 aagaaatgga taagggattt atggatattg cccaattaaa gaaggtaatt gatcaggctt
     2581 atccatatat cgattctgtc gggcttacag gcttaggtga aacactgctt tataagcagt
     2641 tgcctgaagc attggcatat atccgttcaa aaaataaagg catcatcacc accatatcaa
     2701 taaatgcgca cctgcccaat agcgttgagt tggttgaaaa aattgccgat gatctggata
     2761 cacttcaaat ctctatggat ggagtaggag aggtatatga acaagtgcgg ctgcgtggcg
     2821 agttcagttt gttttatgat aacgttaaac gtattgttga actatgcaaa aatcgcagag
     2881 ctgatgtcat gtttaatttc gttgccatca aagaaaacta tttcaacatg gccgaagtag
     2941 ttgaagtcgc aagcgaactt ggcgttgatt atatcaatgt tacgccattc aatgttgcag
     3001 cagttacggc tcacgatatt tcttattacg acttctttca ttccgatgcc ttcaaaaatg
     3061 aacttaaacg agcaaaggat aaggccgacg aagtgaaaaa tgtaacgctc accatttggg
     3121 atataaaaac acctaatacc taccaaaaat gtcatctccc gtggagccac ttctatattt
     3181 catgggatgg atatgctaca ccttgttgcg caaaaccatt tccaaaggag ttgaattttg
     3241 gcaacatttt cgatgatggg ttgatgtatt gtctcaatac tcccggctat cgcgaattcc
     3301 gaaggctttg gtacgaaaat aaaacaccac aattctgcga aaaatgccat atggtagatc
     3361 ttacccccat cgaccttcca tttgaattgc cagtgaatta aaggaaataa tcccttacga
     3421 ataatgccgc taatagtatt gcacctatta gaaacatcca tttaaatcgc ttcctgctgc
     3481 gggcaaagat ttcctcaaat cgttgttgta cctcagcttt gaatttaaaa acaccccaca
     3541 tccacaatgc accgactatc atgaacaata aagtgagtat atcaatcaac aatgtaaagg
     3601 ttgaagcggt catgctaggt tatttgaagt aaaatggtat ttttgaaatt ctaaacccaa
     3661 agttacgcgt ttatctgcca ctatattatt cttgtttctg acagtgcttt cctgtcaggc
     3721 ccagatttgg acacttgaaa aaaatggtgt tcaggcctca gcaagttata cctggtttca
     3781 ctatgataag gcctatcagg ataattctac aaccatagat ctgcccttga ccgtatttga
     3841 taaaactttg gcattggacg tacaatatgg tatcaccgac agaattaccg ccttagccag
     3901 gttgccatat aaaatgttgg aaacaacagg ttatcccgga gtatacaaca gttatccggg
     3961 gcaatatatc gagacaggta agctcaataa catcggcaat acagaatttg gactagtttg
     4021 gaaatgttat gacgataaac ccatcgtttc cgcatcattt attgttgaag caaatacctc
     4081 cgacagaaat tatatcaccg gactgcaaac cggattcaat agctgggctt ttcgtcccgg
     4141 cataggcgtc ggttggggat ttgataaatc atggctccag ttttataccg gtatgtctat
     4201 aagaacaaat gattatgcac ttgcggtagt ttcaaacctg gaagccggat atcattttgc
     4261 tgattactgg tggggtgcca ttagcctgga tacacgcaaa ccaaacgtag atggtgagga
     4321 ttgcgattgt tctacctctg ctactatgct gtatcaaaat gcgcaagaat attacgcact
     4381 ggcattaaaa accggattta caatgtataa agactggggt ttgaatttcg gctatcatct
     4441 cggacttaca ggaaatgatg tcgccgctac tgctgttcct gtgataggcg taatgtataa
     4501 gtcaagacca atgcgacata ctatcattac tcctcgcttt taaccttcat cgcttttcca
     4561 gctgatcctg cttgcggatg atctccagca atttttcatg aatattcaat acttgtctaa
     4621 ttaatggtgc tgaaccatcg ttgtacagga tgaaatcact tttttctaat ttctcttctt
     4681 ccgacatctg attggccatg cgtgagcgca cttgttcttc tgtggtgtta tcgcgttgca
     4741 ttacacgttg aatgcgcaaa tcttccggtg ctgtaacaca aatcacttta tctagttgtt
     4801 tgtttccgcc actttcaaat aataacgcag cttcttttat aacataagga tattgcaaat
     4861 gcctttgcat ccatttctcg ctatctagta atactgctgg atgaattatt ttattgtact
     4921 gtgctaaaaa ttcaggttcg tgaaatacga caaatgctac tcgctttctg tcgagtacat
     4981 gattgctgtc ataaacgtct tcaccaaata aatctttaac acgcttaata atttcagcat
     5041 ccgtttgaag tatctccttt gcacgctcgt cggcataata aacaggcaca cctaatacct
     5101 caaatatttt gcaaactgtc gatttgccgc tgcctatacc tcctgtaatg ccaaccttta
     5161 tcatgtcacc ttgttatcgg aataaacgcc ggataaacgc caatagtatg cagtcccttc
     5221 cttttagcgc gtgccacaat atgatattgg gtgccatcag cagttttttc gaaggtaatg
     5281 ttaaaatctt tagcacttat taacgacgaa acttctgcag cactgctata ttgaatacgt
     5341 acatctactg catgattagt aggtgtattg atagtcaaat acgcatttgc ctcaatcgcc
     5401 gcttctatct ctgcatgcca atcaaccaat attgccgata aacgtatgct gccaaccgca
     5461 ggaaagggga gaggtgtaat gccatctatt attgaatcac cattcccggc tattgcctct
     5521 gtagaccagt aggtaatatc cgtaatcaga cttttcggcc cacgaatggt aatgctgtca
     5581 ggatgtatat aaatactatc aacaacgata tccgttctat ccatgaaatc agcattcaat
     5641 actatcggaa cctttcgctc tgccaagggc tccaaatgta aatctatagt atcaggaatc
     5701 acctgcacta tatgcatatt ccccggaagc cccgccgtaa acaatccaag atgattattt
     5761 gtcaataata ccggtttgtc atcaagatcc gaaacatcta atgacatcgc acgatgacga
     5821 aatccctctt ttactaattg ccatcctgaa gaggatacca gcaaatgggc ttcttgaggt
     5881 aatggtattg ttgtagtctg actttcgggc aatacatagg ataaaccaac attgatttct
     5941 tgttcataat tttcactgag cgcagtaaat aaccaaataa ttactgctat gaataaacaa
     6001 acaaaaaaca atgcaaattc accggcaaat gagcgttgtg cagccggggt gctgtcgccc
     6061 atagtttcca tgcagtatta accttgctta cgcgctaaca ttgctcttgt gaaatccaaa
     6121 gagatcacag atttttcaat gcgcaatttg gtgtttgtgt caacctctat taaataggtg
     6181 tcatcgtcaa ttttaagaat ctttgcatga acaccaccta tggtaacaat cttatcacct
     6241 actttgattt catttaaaaa gttcttttgc tcctttgctt ttttagattg cggacggatc
     6301 atgaaaaaat acagaatcag gaagaacaga aagaaaaatg cgatctgcac ccagctagag
     6361 cccgatgatt gcccgtctgc tgccggcgcc ataagaagta cattgagtat tgacatatct
     6421 ttatttgctt ttaattatta atttgttggt gttataattg ttcctttgat aataagctta
     6481 tttggatttg taaccgtatt cgcaattaca gtaaccgttt tattgaattc tccgcttctg
     6541 ttgccgctat tgaaaacaac tttgataaca ccactgccac cgggaggtat aggctctttc
     6601 ggccattcag gcaccgtgca tccgcaactg ccatgcgcat cggatatcaa taagtcattt
     6661 ttacccgtgt tcatgaattt aaactctgta gaaacctttt cgcccttgat aatttcacca
     6721 aagtcatgca tgtcgcgctc aaaggtgatg gatgtctgat tctttactaa atcagcattt
     6781 aatttttcat cactgccgga tttggcctta acgtcaaccg gcgattgctc cttgcaggac
     6841 aataatatgc ctgcaccgaa aattaaagcg aagcttgcaa ataatacctg ttttttcata
     6901 tggttgatga ttgcttattc tattaatccg cgtccggttt ttacaatgcg gccactactt
     6961 ttcatttcat tcatcagctt atccaaaatg ccattgatga actcgcgact ccttggtgtg
     7021 ctatataatt tgctgatatc gagatactca ttaatagtaa cttttgtcgg caccgttggt
     7081 aaatgcaaaa attctgtcaa tgccattttc atcagaatcc ggtcaatcaa tgaaacacga
     7141 tcgagttccc aattctgaag cttaggtttg atttcctcat catattgcaa atcgctctcc
     7201 atagtaaaca ctaccagcga ctctgcaaat gccatttcct catcgccaac ctgaaaatca
     7261 aacaggtgtt tttcagtttg ctcagatatg ttattattga agaattcatc catcaatccc
     7321 ttcacaataa attcatcatc tgcccaggtc gcaatgctgt tttccatgtg ttcatcgaaa
     7381 tattcgttct ttaaaagaaa atgttcgaat atgtctttga gcatcttttt gtcggcaacc
     7441 aaatcaggcg atgatccaac atagttgtta tagggtgcgt attcttttaa ttgctgataa
     7501 atgatcttac tatactcttc atctaccaaa tcagttaatt tctcacgctt taatcgcgat
     7561 tgaaaataat tgtttttcaa caagccctga ataaccttat tatcaaaaaa tgtcgtcgga
     7621 aacgcaagat cctcggcact cggcaaatac ttgttcttct taatatgtgc ctccaaatca
     7681 acttgctgcg ccaactcagt caggaaatgc aaaatatata aataagcaag atgcgaattg
     7741 cgtatactct tactcaaatg agacaatgca gccttaggct ccggctgacc ggaacatgca
     7801 gctacatata atgtctgcat tgcttttatc cttaatcctc ttctactcaa catgtcaaat
     7861 tacgcagtgt tttttaaggg gtaatactgc aagtccctgc ttatagacgt gcattgatgt
     7921 tcgctattgc acgaatgcgt tcctcagcaa cctggttggc ggcagcttga gtggtaatat
     7981 tatttttcgc agcaacctgg aaaatctcca tcgcgcgatc gtaaatgatc tctgttttag
     8041 acaatgctcg ctcgcggttg tatccttcca attcaacata acagttgatg agtccgcctg
     8101 cattgatcaa ataatccggc gcataggtaa tacctctttc aatcaatcgc ttgccatgaa
     8161 cagcttcgtc cttaagttgg ttattcgctg caccggcaat aatctgacaa ctcaatttat
     8221 tgatggtatc atcgttaata gtggctccca aagcacaagg tgcataaata tccatttggg
     8281 tatcaaatac cttgtccgtt ccaataattt ctgcaccaaa ttcagccgca acagctttta
     8341 atcgctcttc gctgatatca ctgatcatta ccttggcacc gtcttttgtc aggtgtttca
     8401 caaggttgta tcccacatga cctacgccct gaaccaaaat gcgctttccg gataaattgt
     8461 cacttcccgt agcatatttg gttgctgatt tcattcccag ataaacaccg taagcagtca
     8521 caggcgacgg atcgccacct ccacccaaat attcaggttt tcctgctaca tggtctgttt
     8581 ccatattaat gaactccatg tctgatgtcg atgtacctac atcttcagca gtaatatatc
     8641 ttcccgcaag tccattcacg aacttaccat agcgacgaaa caaaggttcg cgattggtaa
     8701 gcgcttttga atcgccaatt atcacggctt tgccacctcc gaggttcaac ccgctgattg
     8761 ctgctttata ggtcatccca cgcgaaaggc gtaaaacatc aataatcgca tcatcttctt
     8821 gctgataact ccagaaacgt gtaccaccca aagcagggcc taatgtagta ttgtgaatag
     8881 cgactatggc tttcagccct gtgttgttgt ctgtgcagaa aaccaattgt tcgtgttcca
     8941 tctcacgcat ggtggtaaac acctctccgt tggttagctg cagggtattg tttgacatat
     9001 aatgaacggt ttttggttgg cgctgcaaag ataggataag ttgtaaaaag aaatagatgt
     9061 tctattagtg gaaaaccttt ttgggtaata tatagttttg cacctgcatg aatgcccttc
     9121 gcccggttca ccaatacctg ctcaaataca aatggctgtt cctcaccgga actttgtttg
     9181 taggtctcgc aaacctgttt caggtatatg ctccgcactt tataggcgat atgattgatg
     9241 ccgtagccga aaatgctgat accgtaatca atggcaccga tagtgaaaaa gcttccgccg
     9301 tttaccgctt cattctcatc aatgcactgc ttttcatcgg aattaccctt cttcgtggag
     9361 tggtgatgtt cttcatgagg caaactatta tcgttatgag ccggaaaatt gaatacgacc
     9421 agaaaaatga actgttccgc aaatatgaat tcttaacccc ggccttcttc aaacgcaata
     9481 ataccggcga tctgatgagc cgtgtggctg aagatgtcgg aagggtaagg caatatgtag
     9541 gaccggccat catgtatttc cttaacctgt tgttcaactt cattttcgga atctggatga
     9601 tgctttccat taacccgaca ctcaccctgt acgtgctgct gccattgcca atattgtcgg
     9661 tttccatcta ttttgtaaat agcattatca acaaaaaaag cgaacaaatt caagcccaat
     9721 tatcgcacct taccagcctt gctcaggagt cattcagcgg tattcgtgta atacaagcct
     9781 atgcgcgtga aaagcaaact gttaactttt ttgcggatgc atgcgatgat tataaaaccg
     9841 actcgcttag ccttgcccgc atcgacgcta tgttccagcc attaatggtg ctgttgattg
     9901 gaatgagcat actgcttacc atttttgtag ggggatggca agtgatacaa ggcaacctga
     9961 gtcccggcaa cctggcggaa tttctgtttt atgtgaatat gatgacctgg cctgttacct
    10021 cgttgggatg ggtggtttcc atcatccaac gcgcagcagc atctcagaaa aggataagcg
    10081 aattcctcga cctgcaaccg gatatcgtat ccgaagggct tgctccaaaa caagtaaagg
    10141 gtcggataac cttcaaaaac gtgcatttta cctatcctga ttcaggtgtt aaagccttgg
    10201 atggagtgag tttcgatata ggaattggcg aaactgtagc tattgttggc agaacaggct
    10261 cagggaaatc aactattgca gacctgattt tcaggctgta tgatgccaac gacggcagta
    10321 tattaattga tggagaaccc atcacatctt tcaacctgac cgatctgcgt aatggagtca
    10381 gtatggtgcc tcaggacgtt ttcttgtttt ctgactcaat agctcacaat atcgcattcg
    10441 ccgcacctga cgcagatgtg gaacgtgtta aacaattcgc tcagtttgcc tcagtcgata
    10501 aagaaattga atcgtttaag tcgggatacg ataccatagt aggggagcga ggtgttaccc
    10561 tcagcggagg tcaaaaacaa cgtgtgtcca ttgcgcgcgc cttcatgaaa gagtctccgg
    10621 ttattattct ggatgattgc ctgagcgccg ttgatgcagg aacagaacat atcatcctgg
    10681 gaaatatgcg tcgctatctg gaaaaacgaa ccgctatcgt aattactcat cgtatcactt
    10741 cattgcttga gtttgataaa atcattgtca tggaccaaca tcaggtggcg gaatatggca
    10801 cacatgatga gcttatggca ctaaatggac tctacgcaaa aatggtaaac gcacagcgcg
    10861 atgtcaccac ttcagaatta aaaaataatg tccgttagtt tgtttttcaa aatcttttta
    10921 tatttcggtc aaaatacaac ggtaaatacc tacctactac cctaaactga ctattgtgga
    10981 gcacgaaaac agcaatcgca acgacagcgt attctcaagg aaaattcgcg ctggaaggcg
    11041 caggacttac ttctttgacg taagaacaac aaaaggaaac gattactaca tcaccattac
    11101 ggaaagcaag aagcgcttcg acggaaatgg atacgataga cacaagctgt ttatttataa
    11161 ggaagacttc aacaaatttg cggatgaact acaacaggta attgatcata tcaaaggtct
    11221 acttcctgat ttcgatttcg acgcattcgc tcatcgatat gatgaaaatg gcgaacatta
    11281 tgatggcggg aatcaccaac atcacgacga tgatcgcgaa caatataacg ataacagtaa
    11341 ttcatcatcc tcggacaata cgtcaacgga taataattcc ggccatcatt atggcgacga
    11401 ggatctgaaa ctctaacaac gacacacaac cggaaagccg ctcctcatgg gcggtttttt
    11461 tgtgtctgtt ggtacgaaaa aaggagattt ttgtcctgtt tttgcacctg acagcatatg
    11521 taacttcggt gcatatgagc aagtggtgta gccggtccat gcaatttcta gttgtaattc
    11581 tcacttttct atcgcttgat agcaaaggcc aaaacctcgt tcccaacaac ggttttgaga
    11641 caaacgacgg tacaccgacc ggttacgggc aatggtatca cgctaccggt tggtctgatg
    11701 taaataacta tcccgccttc ctttggccat acgcaagtcc cgattatttg cacaataccg
    11761 gagcaggcgg agcagatttg ccggtttgca cttttggaac cgtttctccc ttcgcaggca
    11821 atgctgtaat gggtttcgtc acctggcttc aatctacacc tgatttcaga gaatatcttt
    11881 ctataccatt gtccagcccc atgattgtcg gaactgccta taatgtgtcg ttctggataa
    11941 ctaacggctc agcaagttgg tataatggtt attcatccaa tagaattggt gccagattta
    12001 gtattggtac gctaacccag gcaacacacg aaccaatcgg aggaacacct caatgcgagc
    12061 tggttaccga cctgtggacg accacctggc aatacgtaag tttcgtttac acccctgatc
    12121 aagcctataa tcaaattact attggcaact tctataacga tgcatcaaca tcacattcat
    12181 accatgtagc cagtcttagc gcaggagcct actattttat tgatgaagtg gttgtagaac
    12241 ccgctgttcc gcttcctgtc gagttattag cctttgaggc agttcaaacc gaccacggcg
    12301 ctgatatcag ctggaataca gcctcggaat ttgatgtttc atcgtatgtg gtttcaagga
    12361 gtagcgatgg cgtcaatttt aaccctctag gtgagatatc ggccaccggt aacgcaacaa
    12421 cctccgccga ttacctgctg ttggacgatg aacctctttt tggaaacaat tattaccaac
    12481 tcgaagtggt caacctgaat ggagaagtag atgagactta tctaacagcc ttgtatgttg
    12541 atggaaccgc cagccggcct gtcgtctatc caaacccatc aagaggttct gttactatct
    12601 caataccgat aggattacct cttgccggca ttcaaatatt cgacatttca ggacgattaa
    12661 ttcgcgagat taatactcta agcgccacag ttcctatagg tatcaatgac ctgtctacag
    12721 gaacatatgt tatcaaattt atatcgacta ttgatataga taatcaatta ttaatatttg
    12781 aataacaatt acttataaaa attttattta gtaattgcga tttgtcttac tgcaacgtca
    12841 ttgccaataa aggccgccag gaagtaattt ccggcaggaa tattctcgag tgataaacga
    12901 atatctcccg ttgtggtgat ggattgatca aggtagactt gaccggaggc tgtacgtatt
    12961 tgtaatcgtg aaatgttgtc accttcaacc atcagataat tgccggcagg attggggtag
    13021 gctgtaatct caaacaactg ctcactgcca aggttgatga caccggtagg tgttgatgag
    13081 ccaactgctt gcttactgaa ccaaatgttg agatagtcat ttctggtatc tccccaaacg
    13141 gcatacagtg tgtcgttgaa caattgcaca cccataaaat cgttgccatt accgtataat
    13201 acactatcaa aggcagccaa tgcactgcta atacggaaat cgttgctaaa cgctattgaa
    13261 tccttatagc gcacagcccc ccatatctct gatgcgacag catatccggt gtcaacggcg
    13321 ttccgtctgt cgcgccaggt aaccacaaga tttccttctc catcaaaatc gccccaaacc
    13381 aggtcctgca tcaccttatt acctgccgga tctgcattga tacgtaaggg tgtactccag
    13441 ttaactccgc tatttgccga ttcaagacaa taaatgtcat tatctcccaa gggtctgaaa
    13501 acgaacagaa acaccaggtg atgtggatca gtaggatcaa ccaatagctg atatccggtt
    13561 ttggcactgg tatcggcatt caagtcgcca ccaaaggatt ccagcatctc gtaataactg
    13621 aaaccatcgc cgccattaac tgaagatgcg tgaataaacc ttgggagcaa gttttctgtc
    13681 ggttcccagg cagggtagac gcagtgcaag gttccgtcgg gacctacagc cggagcagcc
    13741 atcggggcct gaatgaaatc gccgatctgc cagcctgttg tgtcaatata tcgccaatct
    13801 tcgaatgaca gcccaccatt cttactaacg gtgaggtagt ttctgtttgg gaagggtatc
    13861 catggagcgg gttttgttgt gacatactga aatccgtcat ctaagccgcc actcctgtct
    13921 attaccaacc aaggacgatc tactggcacc tcacccggat cgtcaaaggc atcaattacc
    13981 tccaccggat ctccccatgt aaaaccgcca tctgccgatt tgcggactat cgttttgccg
    14041 gaagtcccga atgggctgta atcgatataa cacagataca ggtttccgtc actatcccaa
    14101 tccatagatg gatccgccga ggtgtatccc cctgcaatat gtggttgcgc atattcagcc
    14161 gaccagctat tgcctccatc cgtgcttacc cgagtcatga tctgaaccag gtccaatccg
    14221 tttaccccca tccaggcaac aaccatatgt gaaccattac ttgggttaat cgttataaat
    14281 ggttcaccat caaatagata tccatctgaa atattcgtat tttgacctcg gacctcacaa
    14341 attggtataa gaattgtgat gatcaataga taggccgata tatttcctag ctgcttcatt
    14401 tgtggttggg tgaatttgtt ttgtctgcag ttaaaggtaa tacctatata gccaatttgt
    14461 gattctttcc tgattcagat ggtagtgagt agtggtatta ggtatcaggt atca
//
LOCUS       DAOQOU010000072        14313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2171_length_14313_cov_5.023215, whole genome shotgun sequence.
ACCESSION   DAOQOU010000072 DAOQOU010000000
VERSION     DAOQOU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14313
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_14313_cov_5.023215"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1626)
                     /locus_tag="PLM90_07750"
     CDS             complement(232..1626)
                     /locus_tag="PLM90_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89304.1"
                     /translation="MRNSSIIRSGLLMAFVLLTGSMFAQYGEINYYRPYDKGGLNVFE
                     TSKESTVPYDGFKLHISGGFTQQYQNLSHSNTATAVLKDNTLTPTTGDSVNINALYPL
                     VGGFNLATANLIIGAQLADGIYVNLENYMSARHHNEFWVKGGYIQIDKLPMFGNPQWF
                     TDNFTVRVGDMEVNYGDAHFYRTDNANGIYNPFVGENIMDAFATEVAGEFYYKPSNGI
                     IAMLGLSGGLLNSDVKVFVDPNTGDTISRKPSIYGKLGYDKKLNDDLRVRLTASLYMN
                     GGTSRNTLYGGDRAGSRYYYAGETSYYMSGSTLTAATATNKAFSGRIDPGFTSKVTSI
                     MINPFVKFHGLEVFATYEMSSGNASLSNTLDSTERSFSQLAASVVYRFLKDEQLYVGA
                     RYNTVSGRLYGYDDTAGDITVNRMQIAAGWFPTENLLLKLEYVNEDYLDFKTDDSRNG
                     LNFKGVMVEASVGF"
     gene            complement(1662..2243)
                     /locus_tag="PLM90_07755"
     CDS             complement(1662..2243)
                     /locus_tag="PLM90_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ89305.1"
                     /translation="MKLAKFIMIIGILVAGIRLQAQNQYTLGSDHKITINGSSNVHDW
                     EETAQTATGDASVIWNTDGSFVIKSFNLKVSVKSIKSDKGSVMDNKTHDALKADEHPY
                     ITFKMLSVKSMTKSGNGWAVKVNGDLTIAGFTKNIDISGMVYVKENGKLYIDVSRALK
                     MTDFKIDPPTALMGTMQVADDITIRFKLNYNMK"
     gene            2437..2844
                     /locus_tag="PLM90_07760"
     CDS             2437..2844
                     /locus_tag="PLM90_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89306.1"
                     /translation="MKKVLIAYFVAVAALLAFSYVALIFSVRFLPGIAEQYFDPIYSN
                     SEKRTFLYFIHPFIVALALKYFWQRFRHMFKGRSVMRGVEVGLIYGFIAVLPAMWITF
                     SGLSVSITIVASWIVYGTIQGMIAGLIYAKINP"
     gene            complement(2911..3837)
                     /locus_tag="PLM90_07765"
     CDS             complement(2911..3837)
                     /locus_tag="PLM90_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ89307.1"
                     /translation="MKKFFKWLGILILGILVIGVIAVVIMINKFSKQSKVEYAVNFTE
                     VALPTDSASLARGAVIAQSTCTSCHGGDFAGTPFFEDEKLGSIPAPNITKGGRTKNYT
                     VKDWQRTLRYGVKPDGHGAFIMPSTEFNHLSDSDLGSLIAYMQTVPASDKQWPDPSMT
                     TLAKILAGAGMFGVMYPASVIDMSDTKPRTNPPPGPTVEYGAYTVAFHGCKTCHGEQL
                     NGYISPDPLSPPGANITPAGNIGKWSLDQFKNTLRTGETPEGKKLDTKFMPWAAMGLM
                     TDMEAEAVFNYLKSMPALDDSEDLAKYKEKNQ"
     gene            4133..5497
                     /locus_tag="PLM90_07770"
     CDS             4133..5497
                     /locus_tag="PLM90_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ89308.1"
                     /translation="MWHKNILDTIGNTPLVQLNNIVKGIDATVLAKVETFNPGNSIKD
                     RMALKMIEDAEKDGRLQPGGTIIEGTSGNTGMGLAIAAIIKGYKCVFTTTDKQSIEKV
                     NALKAFGAEVIVCPTDVDPEDPRSYYSVSSRLVREIPNGWKPNQYDNPSNAKAHYLST
                     GPEIWQQTEGKITHFVAGVGTGGTICGTGRYLKEQNPDIQVFGIDTYGSVFKKYKETG
                     IFDKNEIYPYVTEGIGEDFLPENVDFSVIDRFEKVTDKEAAIMTARITREEGIWVGNS
                     SGSALQGLLQLKDQFKKGDVVVVIFVDHGTRYLGKMFNPSWMMKMGYVDKSGLTARDL
                     ISSNQAGNLISVDAHTTVSQSLQIMMENDFSQLPVTDGGRIVGAINESTLYQQVVKDP
                     ELRMKTVGEVMSAAFPFVDISAPVDALSAMLTPENPALLVRDFKVDKTFIITRYDVMH
                     ALLK"
     gene            5494..6285
                     /locus_tag="PLM90_07775"
     CDS             5494..6285
                     /locus_tag="PLM90_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HMZ89309.1"
                     /translation="MIISDIDAFIASGNFPPKRIISLVPSLTELLVDIGLEDALVGVT
                     KFCVHPVGINKRVQVIGGTKNLRIDLIRSLNPDLIIANKEENEQNQVEALASDVPVYL
                     SEITCVADAYEMMRIVGRLTQTTTTVQQRIFELERAEAGLRQQSSISPPKKVLYLIWR
                     DPWMSAGGDTFIDDMLNCCGWENVVAERRRYPSISDTEIRALEPELVLLSSEPYPFKD
                     KHRAEIEALLPNAKVQLADGEAFSWYGTRMHHSFDYLRNLVAKMA"
     gene            6345..7286
                     /locus_tag="PLM90_07780"
     CDS             6345..7286
                     /locus_tag="PLM90_07780"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMZ89310.1"
                     /translation="MVTNDVKLFSGTTSRYMAEKVADYYGQPLGKMTIDKFSDGEFQP
                     NILESVRGSYVFLIQSTMFPSDNLMELLLMIDAAKRASASYITAVIPYFGLARQDRKD
                     KPRVSIGSKLVAELLTAAGANRIVTMDLHADQIQGFFDIPVDHLSSTAIYVPYIEENL
                     DLTNVIFASPDVGSTKRNRRYAMHFNTELVICDKYRKKANEIAEMTVIGDVSGKDVIM
                     IDDIVDTGNTLANAANLMKQKGAKTVRAFCTHPVLSGKAYETIENSSLEEIVVTDTIP
                     LKRESSKIRVLSSAKLFARAIRNTHEHRSISSLFINN"
     gene            7304..7915
                     /locus_tag="PLM90_07785"
     CDS             7304..7915
                     /locus_tag="PLM90_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HMZ89311.1"
                     /translation="MQTFQLEGKVRDHVGRRSAMDARAESMVPCVLYGDGENIHFEVA
                     NSAIKNLVYTPNVYKVHVTVNGATHECLMREIQFHKVTDEILHIDFLKLNPAKKVTCV
                     VPVKLEGQSTGVKNGGKLVQRLKKLSIKALPKDLVDKVTLDITDLEINKTARIGDINI
                     ANVEVLGSKSIPIATIASPRALKTAATDDKGAAAAAPAAEKKA"
     gene            7963..8586
                     /gene="pth"
                     /locus_tag="PLM90_07790"
     CDS             7963..8586
                     /gene="pth"
                     /locus_tag="PLM90_07790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMZ89312.1"
                     /translation="MKLLRFLFSKKQPTPSTTVEPDMKFLIACLGNIGADYDFTRHNI
                     GFDVADHLAAKHNVSFTSGRYADVAEIRIKGRQLTVIKPTTYMNLSGKAVKYWMDKEK
                     IAIEHLIVVTDDINLPLGTLRIRKKGSDGGHNGLKNITEILGSDQYPRLRFGAGNNFP
                     KGAQVHFVLGKWESEEEADVRTNIERAGNAVISMVLEGIDRAMNTYN"
     gene            complement(8603..9526)
                     /locus_tag="PLM90_07795"
     CDS             complement(8603..9526)
                     /locus_tag="PLM90_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ89313.1"
                     /translation="MRFSIKHLILSSFCLLMMLPSLQAQRQLGSKPMGNGQPGSAGKS
                     FDHIEGTYAVVNGINMYYEQYGEGDPILLIHGNGGSTESFDGQIAFLSRKYKVIVADS
                     RGQGRSTNNADSLTYELMAEDFYLFLEQLELDSVNIVGWSDGGIIGLILARDYPEKVK
                     KLVAVGANLRPDTTAVSEALLDWTRMKMEQAQDSVAAGKKSFATEEMLMRLMLHHPNI
                     DVADLHDIKAPVMVVAGDHDLIKVTHTVEIYENLPHAQLAIYPGATHNLMLESPQLFN
                     NAVLRFFSRPYVAKDPMSVMLDTPSKNNDEE"
     gene            9597..10205
                     /locus_tag="PLM90_07800"
     CDS             9597..10205
                     /locus_tag="PLM90_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMZ89314.1"
                     /translation="MKIFCVGRNYGEHAKELNNAIPEAPVIFMKPPTAILKGNDFYMP
                     DFSKDMHFECELVFRICKNGKHIAPQFASKYVDGITVGIDFTARDVQAEQKKKGLPWE
                     IAKAFDQSAVVGTMQPLEDMSLLSRASFELKKNGVAVQTGNAGEMLFPVPEVISYLSN
                     YFTLQQGDLLFTGTPAGVGPIAIGDVLEGSLNGNVLFTTHIK"
     gene            10225..12303
                     /locus_tag="PLM90_07805"
     CDS             10225..12303
                     /locus_tag="PLM90_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMZ89315.1"
                     /translation="MNQKETLEMIDRKVLLEAYRKMYTSKVMSDIFNENRQVCVFVHA
                     NSRGHEAIQLAMGMQLKKSDWASLYYRDDAFLMGMGWQPAQIMLQLMAKRDDPFSGGR
                     NYYCHPSYKGPDYPQMIHQSSATGMQAIPTTGIAQGIQYLEQQNLLATSNDHPLVVCS
                     IGDGAMTEGEVSEALQFSVLKGLPIIYLVQDNDWGISVSREEARTMDAYEYAGGFKGL
                     RRVRVDGADFAESWQCVKDAIGYVRVHRKPVLIHAKVPLLGHHTSGVRMEMYRSEEDL
                     KEHASRDPLPRLRQVLVDAGISSQEITTIETEADQQTRADFQTVLASADPDPATVSDF
                     EFAPSPYTEEQGTREPQGADVVPMVDAALFAMDEILTKHPEALFYGQDVGRRLGGVFR
                     EAATLAAKHGDHRVFNTAIQEAYLVGSTAGMSAVGAKPVVEIQFADYIWTGVNQLVSE
                     LSKSYYLTNGKWNVQSIIRVPIGAYGSGGPYHSGSIESSITAIKGIKVVYPSNAADMK
                     GLLKGAFYDPNPVVMFEHKGLYWSKVPGTAAAKNIQPADDYIIPLGKARIFQHADDKH
                     IHNGESMVVITYGMGVHWALNASKQFPGQVEILDLRTLYPYDWEAITASVKRHNKCFV
                     LTEEPLINSYAQAMSGRIGSELFRYLDAPVRTLGAKDLPAVPLNSGLERAMLPNVEQV
                     KTVMQELLSF"
     gene            complement(12340..>14313)
                     /locus_tag="PLM90_07810"
     CDS             complement(12340..>14313)
                     /locus_tag="PLM90_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ89316.1"
                     /translation="IRGIWPAAYCPEGTDDIYVVNADTQFVTGLNFSRSDDGGATWAV
                     LNSALPFLTVADGMPAITNGAENYQVVAHGSDVYVLFGMINSDLILMHSSEYGNEGTW
                     EKITIIDFPFDNYTGTVQTDVDGDLITDTIGTTDGSHFMTITDDGTVHVFSPYYRIYS
                     DLGAFFWTVNWNTMGLWHWSSGMPAAEVIDLEMDWINADCSNDPYVGIGASTFNYRNA
                     AYVTDPGAAYDPISGRMFLLYTMKIEYTDVFGDPTNPSAQSFHDIFGTYSDDGGATWA
                     QPVNLTNTAETGEENFFMYVYDRVVDGKVYAVWQQDGEPGHFNEGDPITVNNIKFRAW
                     DIESFNPTLPTAAYLFVTDIGEVTFTNISEDATGCNFWDFGDGTTSNESDPVHIYTVA
                     GEYTVCLTAENPYGTDTYCGTIYISLPPDAAFSYTGDPIVTFTDLTLNDPTSWSWNFG
                     DATTSTLQNPVHTFTSEGTFTVCLTATNALGFEVYCLPVTIDSTELLAPAADYSFTVA
                     GGTTVNFTDMSTNAPTSWAWDFGDGGSSTEQNPSHAFPSSSSYEVCLIAANAFGSDTV
                     CKTVLGSEVIDLSDMITLQPNPAHDIVTIHTGTFHPDMIHVYDITGREVMVEVQLSAN
                     DTYVMQVSSLPQGAYIVHMQHENESGYARLIVQ"
BASE COUNT         4154 a         2905 c         3177 g         4077 t
ORIGIN      
        1 tccctattaa cgcgccagcg ttaaaagcac tccacattgg ctaatcggct aatcagctaa
       61 ttggctaatt aaccacactt gctaacttgc taaccctcaa acttgctcac taagacgaga
      121 gtctccatgc aagcacactc ccgatttgct aatctgctaa ttcccaaatt tgctaattaa
      181 aaaaacccgc actgtcgaaa caatgcgggt taacccaatt aaaacccttg atcagaatcc
      241 tacagaagct tcaaccatca cacctttgaa attcaaacca ttacgagaat cgtcggtttt
      301 gaaatcaagg taatcttcat ttacatattc aagcttcaat aacagatttt ctgttgggaa
      361 ccagcctgca gcaatttgca tgcggttaac agtaatatca cctgcagtgt cgtcgtaacc
      421 atacaaacga ccggaaacag tattgtaccg agcaccaaca taaagttgtt catctttcag
      481 gaagcggtaa acaactgagg cagcaagttg actgaagctg cgttcggttg aatctaaagt
      541 attgctcaat gatgcattac cacttgacat ttcgtaggta gcgaatactt ccaatccatg
      601 gaatttcaca aatggattga tcataatgct tgttactttg ctagtaaatc ccggatcaat
      661 acggccggag aatgctttat tggttgcagt tgctgcagtc aatgtagatc ctgacatata
      721 gtatgaagtt tcacctgcat aatagtagcg agaacctgcg cggtcgccgc catataatgt
      781 attgcgtgaa gttccaccgt tcatatataa tgaagcagta agacgtacgc gaagatcatc
      841 attcaatttt ttgtcataac ccaatttacc ataaatagat ggtttacggc tgatagtatc
      901 accggtattt ggatcaacaa atactttcac atctgaattc agtaatccac ctgacaaacc
      961 taacatggca ataataccat tcgatggttt gtaataaaat tcacctgcca cttcagttgc
     1021 aaatgcatcc attatatttt cgccaacaaa tggattataa ataccgttag cattgtctgt
     1081 gcgatagaag tgtgcatcgc catagtttac ttccatatca ccaactctaa ctgtgaagtt
     1141 gtcggtaaac cattgtggat ttccgaacat tggcaattta tcaatttgga tatatccgcc
     1201 ttttacccag aattcgttgt ggtgacgagc agacatatag ttttcaaggt ttacataaat
     1261 accatctgcc aattgcgcac cgatgataag gtttgcagtt gccaggttga aaccaccaac
     1321 cagtggatac agtgcattga tgttcactga atcgcctgtt gtaggagtaa gggtattatc
     1381 tttcaacacg gcagttgctg tattgctgtg agaaagattt tgatattgct gtgtgaatcc
     1441 accgctgata tgcagtttga atccatcgta aggaactgtt gattccttgc ttgtttcaaa
     1501 tacgttaaga ccacctttat catatggtct ataataattg atctcgccat attgggcgaa
     1561 catactgccg gttaacagga caaaggccat taacaaaccg gatctaatga tgcttgagtt
     1621 tctcataata tataggtttt atagataggt tataaaaatg attatttcat gttgtaattt
     1681 aatttgaatc gtatggtgat gtcgtctgcc acttgcatgg tacccatcag tgctgttgga
     1741 ggatcgattt taaaatcggt cattttcaaa gctctgctca catcgatata cagcttaccg
     1801 ttttctttaa cgtaaaccat tccgctgata tcaatatttt tggtgaaccc tgcaatggtt
     1861 aaatcaccat tcacttttac tgcccagcca tttccacttt ttgtcatgga tttaactgat
     1921 agcattttaa aggtgatata gggatgttca tcggctttca gtgcatcatg tgttttattg
     1981 tccattactg aacctttatc acttttaatg ctttttaccg atacctttaa attgaaagat
     2041 ttgatcacaa atgaaccatc ggtattccaa ataactgaag catcgccggt agcagtttgt
     2101 gctgtctctt cccagtcatg tacattgctt gagccattaa tagttatctt atgatcagaa
     2161 ccgagggtat attggttttg cgcctgtaat cttataccgg caaccagtat cccgattatc
     2221 atgatgaatt ttgccagttt catatcttaa ggttttgatg catttaattg acaatgcaaa
     2281 ataagttacc ggcggaacct caactgatta taatagtgtt cgccgaaagg gacttatgtc
     2341 atcttttgac gatggtatct gcgcaacctt tgtgcaaggt tttatctaac aaattggtca
     2401 tattgttaaa aatataacgt gtcaaaacct actaatatga aaaaagtact tatcgcctat
     2461 tttgttgccg ttgcagcact attggcattc agctatgtag cattgatttt ctcagtgcgt
     2521 tttttaccgg gcattgcgga acaatatttt gatccgattt attccaacag cgaaaaacgc
     2581 accttcctgt attttataca tccattcatc gtggcgctgg cattaaaata tttctggcag
     2641 cgattccgcc acatgttcaa aggaagatct gtaatgcgcg gagtggaagt tggactgatc
     2701 tatggcttta ttgcggtatt acctgccatg tggatcactt tcagcggttt atccgtttca
     2761 attacaattg ttgcttcctg gatagtttac ggcactattc aaggtatgat agccggtctt
     2821 atttacgcta agatcaatcc ttaaaatctg aaattctatt caataaaaaa gcccgaagta
     2881 tacctccggg ctttttcgtt atcagtattg tcactgattt ttttccttgt attttgccaa
     2941 gtcttcacta tcatcaagtg caggcatgct ttttaaataa ttaaatactg cttctgcttc
     3001 catatcggtc atcagaccca tcgctgccca aggcataaat ttggtatcga gtttcttccc
     3061 ttcaggcgtt tcacctgtgc gcagtgtatt tttaaattga tcaagtgacc atttaccaat
     3121 atttcctgcc ggagtaatgt ttgcccccgg gggagaaagc gggtcaggtg aaatgtatcc
     3181 attcaattgc tcgccgtgac atgttttgca gccatgaaac gccaccgtat atgctccgta
     3241 ttcaactgtt ggtccgggag gtgggtttgt gcgtggttta gtgtctgaca tatcgataac
     3301 ggatgcagga tacattacgc caaacattcc ggctccggca aggatctttg ctaaggtagt
     3361 catcgaagga tcgggccatt gcttgtcaga tgccggaaca gtctgcatat atgcaatcaa
     3421 tgaaccaaga tcactatcac tcaggtgatt gaattccgta cttggcatga taaaagcacc
     3481 atgtccatca ggtttaacgc cgtaacgcag tgtgcgttgc caatctttta cagtgtaatt
     3541 tttagtacgt ccgcctttgg tgatattcgg tgccgggata cttcctaact tttcatcctc
     3601 gaaaaatggt gtccctgcaa aatcaccgcc gtggcaactc gtgcaggttg attgagcgat
     3661 aaccgcgcca cgagctaatg acgcactatc agttggtaag gcaacttcgg taaagttgac
     3721 tgcatactca accttcgact gtttagagaa tttgttaatc atgatcacaa cagcaatcac
     3781 gccgatgacc agaatgccaa gtataagaat acccagccat ttgaagaatt ttttcatgtg
     3841 taatggtgtg cttgtatcca ccacgggcct gtattccgcg gcagagggtt gtcaaatgat
     3901 atggaaaatt actttttttc gggtgatttt gcaaataagg gtgagttgcg cgcaaagacg
     3961 ctaagtagca aaggcgcaaa gaaattcacc actcacccta tttcaatcgc ggagtttgcg
     4021 gaggaatctc actcacgagt cacgagtcac gaatcacgta tcacgattta aacactcacc
     4081 attcacaacc cgccgtggcg aggcaagctc accttttcct acctttgcgc ttatgtggca
     4141 taaaaatatc ttagatacca tcggaaacac cccattggtg cagttaaaca atatcgtaaa
     4201 gggcattgat gcaacggtat tggcaaaagt tgaaactttt aaccctggaa attccatcaa
     4261 agaccgcatg gctttgaaga tgattgagga tgctgaaaag gacggcagat tgcaaccggg
     4321 aggtaccatt attgaaggca cttcaggaaa tactggaatg ggacttgcca ttgcggcaat
     4381 tatcaaagga tataaatgtg tatttacgac caccgacaag cagagtattg aaaaggtaaa
     4441 tgcccttaaa gcattcggtg ctgaagtaat tgtttgtcca acggatgttg atcctgaaga
     4501 tccaagaagt tattattctg tttcatctcg attggtgcgt gaaattccta atggatggaa
     4561 accaaatcaa tatgataatc cgagtaatgc aaaagcgcat tatttaagta cgggtccgga
     4621 aatatggcaa caaaccgaag gcaagatcac gcattttgtt gcgggagttg ggaccggtgg
     4681 taccatttgc ggaaccggca ggtatttgaa agagcaaaat ccagatattc aagtttttgg
     4741 tattgatact tatggctcgg tatttaaaaa atataaggag acaggtattt ttgataagaa
     4801 cgaaatttat ccttatgtaa cagaaggcat tggtgaagat tttttacctg agaatgtaga
     4861 tttcagtgtg atagacaggt ttgaaaaagt gaccgataaa gaggccgcca tcatgactgc
     4921 gcgcattaca cgcgaagaag gcatatgggt tggaaactcg agtggctctg cattacaggg
     4981 attgctgcaa ttaaaagatc aattcaaaaa aggcgatgtt gtagtagtga tatttgttga
     5041 ccatggcacg cgctatcttg gaaaaatgtt taacccgtcg tggatgatga aaatgggtta
     5101 tgtagataaa tcaggtctta ccgcgcgcga tttgatcagc agcaatcagg caggaaattt
     5161 aatttctgtt gatgcgcata ctaccgtttc gcaatcgctg cagattatga tggaaaatga
     5221 tttttcacaa ttacctgtaa cggatggcgg aagaattgtt ggtgccatta atgaatcaac
     5281 tttatatcag caggtcgtaa aagatccgga gttgcgtatg aaaacagttg gcgaggtaat
     5341 gagtgcagca tttccatttg tagatatatc tgcacctgtt gatgcattaa gtgctatgtt
     5401 gacgcccgaa aatccggcat tattagtgcg cgattttaag gtcgacaaaa catttatcat
     5461 cacgcgatat gatgtgatgc atgccttgtt gaaatgatca tcagcgatat agatgcattt
     5521 attgcttccg gcaattttcc tccgaagcga attatttcct tggtgccttc gctgacggaa
     5581 ttgctggtgg atatcggttt ggaggatgca ctggttggtg ttaccaaatt ttgtgtgcat
     5641 cctgtaggca tcaataaacg tgtgcaggtt atcggtggca caaaaaatct gcgtatagac
     5701 ctcatcagat ccctaaaccc ggatttgatc atagccaaca aggaagagaa cgagcaaaac
     5761 caggttgaag cgctggcttc agatgtgcct gtatacctct cagaaattac atgcgttgcc
     5821 gatgcctacg agatgatgcg gattgttggt cggcttacac aaaccaccac aactgtacaa
     5881 caaaggatct ttgagcttga gagagcagag gccgggttgc gacaacaaag ctcaatttct
     5941 ccccccaaaa aagtgcttta cctcatctgg cgcgaccctt ggatgtcggc aggcggcgac
     6001 acgtttatag acgatatgtt gaactgctgc gggtgggaaa atgtggttgc cgaaaggcgt
     6061 cgataccctt cgattagcga cacggaaata agggcactgg agccggagct ggtgctattg
     6121 tcgagcgaac cttacccttt caaggacaaa catcgtgccg aaattgaagc gttattgcca
     6181 aatgcgaaag tacaacttgc tgacggtgaa gcatttagct ggtatggaac gcgcatgcat
     6241 cacagttttg actatctgcg aaatttagtc gcaaagatgg cttaacactt tcttcatacg
     6301 cggctttttc ctatctttgc gggctcaaac gaacgaatag tccgatggtt accaatgatg
     6361 taaagctgtt ctccggtact accagccgct acatggcgga aaaagttgcc gattactatg
     6421 gccagccgct gggaaagatg accattgaca agttcagtga tggtgagttt cagccgaata
     6481 ttttggagag tgtgcgtgga agttatgtgt ttcttattca gagcaccatg tttccttcgg
     6541 acaatctgat ggaattattg ttgatgatag atgctgcaaa acgcgcttct gcttcgtaca
     6601 tcactgccgt aattccgtat ttcggattgg cgcgtcagga tagaaaagac aagccacgtg
     6661 tttccatcgg ttctaagttg gtggcggaat tattgactgc tgcgggtgcg aatagaattg
     6721 taacgatgga tttacatgcc gatcagattc aaggtttttt cgatataccg gtagatcacc
     6781 tgagttcaac ggcgatatat gtgccatata ttgaagagaa cctcgatttg accaatgtaa
     6841 tttttgcatc accggatgta ggcagcacga aacgcaatcg tcgctatgcc atgcatttca
     6901 atactgaatt ggttatttgc gacaagtatc gcaagaaggc caatgagatt gctgagatga
     6961 cggttatcgg tgatgtttcg ggtaaagatg taatcatgat cgacgatatt gttgatacgg
     7021 gtaacacgct tgccaatgct gccaatctga tgaagcagaa aggcgcaaaa acagtacgtg
     7081 ctttttgtac gcatccggta ttatcgggca aggcgtatga aacaattgaa aattcgtcgc
     7141 ttgaagagat tgtggtaacg gataccattc cgttgaaacg agagtcgtcg aaaatcaggg
     7201 tgttgtcatc ggcaaaatta tttgcacgcg cgatacgcaa cacgcatgag cacagaagta
     7261 tcagttcgct ttttattaat aactaaacaa taactcaatc acaatgcaaa catttcaatt
     7321 agaaggcaag gtgagagatc atgtagggcg ccgttcagca atggatgcac gcgcggaaag
     7381 catggtgcct tgtgttctct atggtgatgg cgaaaacatc catttcgaag tagccaattc
     7441 agcgattaaa aatttggtgt atacgcctaa cgtttacaaa gttcacgtaa ccgttaacgg
     7501 tgcaacacac gaatgcttga tgcgcgaaat tcagtttcac aaagtgactg atgagatctt
     7561 gcacatcgat tttctgaaat taaatcctgc caaaaaagtt acctgtgtgg tacctgttaa
     7621 attggaaggt cagtctactg gtgttaaaaa tggtggtaag cttgttcaac gtttgaaaaa
     7681 actgagcatt aaagcattgc caaaagatct cgtagacaag gttacactcg acattacaga
     7741 tctcgaaatc aacaaaacgg cacgtatcgg cgatatcaat attgccaacg tagaagttct
     7801 cggttctaag agcattccta ttgccaccat agcatcacca cgcgcgctga aaacagctgc
     7861 aactgatgac aaaggtgccg cagcagcagc tcctgcagca gagaagaaag cctgatccgt
     7921 tcagcatact taacgaagca atttctgtta acttagcagt acatgaaatt gcttcgtttt
     7981 ttattttcca aaaagcaacc cactccttca actactgttg agcctgacat gaaattcctt
     8041 atcgcatgcc tcggaaatat tggtgccgat tatgatttca cacgtcataa tatcggtttc
     8101 gatgttgcag atcatctcgc tgcgaaacac aatgtatcct ttacatcagg aagatatgcc
     8161 gatgttgcgg agatacgcat taaaggaaga cagctaaccg taataaagcc aaccacttat
     8221 atgaatctca gtggcaaggc ggtgaaatac tggatggata aagagaagat tgctattgaa
     8281 catctcattg ttgttaccga cgatattaat ctgccgttag gcactctgcg catcagaaaa
     8341 aaaggcagtg atggaggtca taacggatta aaaaatatca cagaaatttt gggttccgat
     8401 caatatccgc gactgcgatt tggtgcagga aataatttcc ccaaaggcgc acaagtacat
     8461 tttgttttag gaaaatggga aagcgaggaa gaagcagatg tgcgcaccaa tattgaacgc
     8521 gcaggaaatg ccgtaatcag catggtgctt gagggaatag accgcgcaat gaatacttac
     8581 aattaaccgt gaaggttaat tattattctt catcattatt tttcgaaggg gtatctaaca
     8641 tcaccgacat cgggtcttta gcaacataag gtctggagaa aaatcgcaat acggcgttgt
     8701 taaataattg cggtgattcc agcatgagat tatgtgttgc acccggatat atcgctaatt
     8761 gagcatgcgg aagattttcg tagatctcaa ctgtatgcgt aacttttatc agatcgtgat
     8821 cgccggcgac taccattacg ggtgctttaa tatcatgcag atcggcaaca tcaatattag
     8881 gatgatgcaa catcaaacgc atgagcattt cttctgtggc gaatgatttt tttcctgccg
     8941 caacagaatc ctgtgcctgt tccattttca tgcgtgtcca gtcgagtaat gcttcactca
     9001 ccgctgtggt atcgggacgc aaatttgcac ctacggcaac aagttttttt actttttcag
     9061 gataatcgcg ggcaaggatc agtcctatga taccaccatc gctccaaccc acaatattta
     9121 cgctatcgag ttccaattgc tccaggaaca gataaaaatc ttccgccatc agttcatacg
     9181 tcaggctatc ggcattatta gttgatctcc cctgccctct tgagtcagca acaataactt
     9241 tatattttct cgagaggaat gcaatttgac cgtcgaagga ttcggttgag ccgccgttgc
     9301 cgtgaatcag caggatggga tccccttcgc cgtattgttc gtaatacata ttaatgccgt
     9361 taaccacagc ataagtacct tcaatatgat cgaatgattt gcctgcagag cccggttgtc
     9421 cgttgcccat tggttttgag cccaattggc gctgtgcctg caatgagggg agcatcatca
     9481 ggaggcaaaa actgcttaaa atcaggtgtt ttattgaaaa tcgcatatac aaagatacag
     9541 gaaatggggt actgctgctg ttggtgcggt atcgcttttg taattttgtg ccgcttatga
     9601 agatcttttg cgttggcagg aattatggcg agcatgccaa ggaattgaat aatgcgattc
     9661 ctgaggctcc ggtcatcttt atgaaaccac ccacagccat tctgaagggc aacgattttt
     9721 acatgcctga tttttcaaaa gatatgcact ttgagtgcga attagtgttc cgtatctgta
     9781 aaaacggtaa gcatattgca ccacaatttg cgagcaagta tgttgatggc attactgttg
     9841 gcattgattt tacagcacga gatgtacagg cagagcaaaa gaagaaagga ttgccatggg
     9901 agattgcgaa agcatttgat caatcagccg tagtgggaac catgcagcca ctggaggata
     9961 tgtcgctgct ttcacgtgca agttttgaat tgaagaaaaa tggcgttgca gtgcaaacag
    10021 ggaatgccgg tgaaatgtta tttcccgtgc cggaggtgat cagttattta agcaactatt
    10081 tcactttaca gcagggcgat ttattattca ccgggacacc ggcaggtgta ggacctattg
    10141 ccattggtga tgtgcttgaa ggatcattga acggcaacgt attatttaca acgcatatta
    10201 aataagtatt tcgtcaatag gataatgaat caaaaagaga cattggaaat gatcgaccgc
    10261 aaggtattgc tggaggcata tcgcaagatg tacaccagca aggtgatgag tgatatattt
    10321 aatgaaaacc gtcaggtatg tgtttttgtg catgccaatt cacgtggtca cgaggcgata
    10381 caactcgcta tgggcatgca attaaaaaag agcgactggg caagtttata ttatcgcgat
    10441 gatgcatttt taatgggcat gggatggcag ccggcgcaaa tcatgttgca attgatggcc
    10501 aagcgcgatg atccatttag cggaggaaga aattattatt gtcaccccag ttataaaggc
    10561 cctgattatc cgcagatgat acatcagtcg tcggcaacag gtatgcaggc tattcctaca
    10621 acaggcattg cgcagggcat tcaatattta gagcaacaaa atttgttggc aacttcgaat
    10681 gatcatcctt tagttgtttg ttcaattggc gatggtgcga tgacagaggg tgaagtttct
    10741 gaagcattac aattttcggt attaaaagga ttaccgatta tttatttggt acaggataat
    10801 gattggggta tcagtgtatc gcgagaagaa gcgcggacaa tggatgctta tgaatatgca
    10861 ggtggattta aaggattgcg cagagtacgc gttgatggtg ctgattttgc tgaaagctgg
    10921 caatgtgtga aagacgccat cggttatgtg cgtgtgcatc gcaaacctgt actcattcat
    10981 gcaaaagtgc cattactcgg acatcataca agtggtgtgc ggatggagat gtatcgcagt
    11041 gaagaagatt tgaaagaaca tgcttcacgt gatccgctgc cgcgactgcg acaggtgttg
    11101 gttgatgcgg gcatttcatc acaagaaata acaacaatag aaacagaagc cgatcaacaa
    11161 acgagagctg attttcaaac agttttagca tcagcagatc ctgatcctgc aacagtatca
    11221 gatttcgaat ttgcaccttc gccatacact gaagaacaag gcacgcgcga accacaaggt
    11281 gctgatgtag tgccgatggt tgatgctgca ttatttgcca tggatgaaat tcttactaaa
    11341 catccggaag cattatttta tggtcaggat gtaggtcgcc gacttggagg tgtatttcgc
    11401 gaagcggcaa cactggctgc aaaacatggc gatcatcgcg tatttaatac tgccattcag
    11461 gaagcttatt tagtaggttc aactgccggt atgagtgctg ttggtgcaaa gccagttgtt
    11521 gaaattcaat tcgctgatta tatatggacg ggcgtgaatc aattggtaag cgaattatcc
    11581 aaatcatatt atctcaccaa tggcaaatgg aatgtgcaat ctatcattcg cgtgcctatt
    11641 ggtgcgtatg gttcgggcgg accttatcac agcggttcta ttgaatcatc tatcactgcc
    11701 attaaaggaa ttaaggttgt atatccgagc aatgccgcag acatgaaggg attattgaaa
    11761 ggtgcatttt atgatcctaa tcctgttgtc atgtttgaac ataagggatt gtattggagt
    11821 aaagtaccgg gaactgcagc tgcaaaaaat attcaaccgg ccgatgatta tatcatacca
    11881 cttggcaaag caagaatttt ccaacatgcc gacgacaagc atattcataa tggcgaaagc
    11941 atggtggtta tcacttacgg catgggcgtg cactgggcgt tgaatgcatc gaaacaattt
    12001 ccaggtcagg tagaaatact cgatttgcgc acattatatc catacgactg ggaagcaatt
    12061 accgcatcgg ttaagcgaca taataaatgt tttgtgttaa cagaagagcc attgatcaat
    12121 tcatatgcac aggcaatgtc gggacgaata ggttctgaat tattccgcta tcttgatgca
    12181 ccggtaagaa cattgggcgc taaagacctt cctgcagttc cattgaattc gggattggag
    12241 cgtgcgatgt tgccgaatgt agaacaggta aaaacagtaa tgcaggaatt actctccttc
    12301 tgaaggtaat tcctgcatta caaaaaatat tctttccggt tactgcacta tcaatctggc
    12361 gtaaccactt tcattttcat gttgcatgtg cacaatgtaa gcgccttgag gcaatgatga
    12421 tacttgcatt acataagtat cattagctga caattgcact tcaaccatta cttcgcgacc
    12481 tgtgatatca tacacatgta tcatatccgg atggaaagtt ccggtatgaa tggtaactat
    12541 atcgtgtgca ggatttggtt gcaaagtaat catatcgctg aggtctatca cttcgctgcc
    12601 taaaactgtt ttacaaacgg tatcacttcc aaatgcgttt gctgcaatca aacaaacttc
    12661 ataagatgat gatgaaggga atgcatgaga aggattttgt tcggtagaag aaccgccatc
    12721 accaaaatcc catgcccatg acgttggagc atttgtagac atgtcggtga aattcactgt
    12781 agtgccacct gcaactgtaa aactataatc agctgccggc gcaagtaatt cggtagaatc
    12841 tatcgtaaca ggcaagcaat atacttcgaa tccgagtgca tttgttgctg taaggcaaac
    12901 agtaaatgta ccttcagaag tgaaagtatg caccggattt tgcaatgtgc tggtagtagc
    12961 atcaccaaaa ttccatgacc atgaagtagg gtcatttaag gtaagatcgg tgaaggtgac
    13021 gataggatcg ccggtatatg aaaatgctgc atcaggagga aggctgatat aaatagttcc
    13081 acaataagta tccgtgccgt atggattttc tgcggtgagg caaaccgtat attcaccggc
    13141 tactgtatag atatgcacgg gatcagattc attactggta gtgccatcac caaaatccca
    13201 gaaattacaa cctgttgcat cttctgaaat atttgtaaaa gttacttcgc caatatcagt
    13261 tacaaataaa tatgctgcag ttggcaatgt cggattgaaa ctttcaatat cccatgcacg
    13321 gaatttaata ttatttactg ttatcggatc gccttcatta aaatgccccg gttcaccatc
    13381 ctgctgccaa acggcgtaaa cttttccatc aacaactcta tcatacacat acataaagaa
    13441 gttttcttca cctgtttctg cagtattggt cagattaaca ggttgtgccc atgttgcacc
    13501 gccgtcatcg ctataggttc cgaaaatatc gtgaaaagat tgtgcggaag ggtttgttgg
    13561 atcaccgaat acatctgtgt attcaatttt catggtgtat aataaaaaca tgcgaccgct
    13621 gataggatca tatgcagcac ccggatcggt gacgtatgct gcattgcgat aattaaaagt
    13681 gctggcacca attccaacat atggatcgtt gctgcaatcg gcattaatcc aatccatttc
    13741 gagatcaatc acttctgcag caggcatacc tgaagaccag tgccataatc ccatggtatt
    13801 ccaatttaca gtccagaaaa aagctccgag atcagaataa atacgatagt atggtgagaa
    13861 tacatgcacg gtaccatcat ccgttatggt cataaaatgg gagccatcgg ttgttccaat
    13921 ggtatcagta attaaatcac cgtcaacatc tgtttgaacg gtacctgtat aattatcaaa
    13981 aggaaaatct atgattgtaa ttttttccca ggtgccttca tttccatatt cagaggagtg
    14041 cattaatatc aggtcgctgt tgatcattcc gaacaacaca tacacatcgc ttccatgagc
    14101 aactacctgg taattttcag caccattggt aattgcgggc atgccatcgg caactgtgag
    14161 aaatggcaat gcggaattca atacggccca ggtagcgccg ccatcatcac tacgtgaaaa
    14221 gttgagaccg gtaacaaatt gtgtatcggc atttaccacg taaatatcat cagttccttc
    14281 cggacaatat gctgccggcc aaatgcctct tat
//
LOCUS       DAOQOU010000073        14304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2174_length_14304_cov_6.227876, whole genome shotgun sequence.
ACCESSION   DAOQOU010000073 DAOQOU010000000
VERSION     DAOQOU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14304
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2174_length_14304_cov_6.227876"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLM90_07815"
     CDS             complement(<1..338)
                     /locus_tag="PLM90_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ89317.1"
                     /translation="MQVKNIAIMGAGLVGSLLSMYLKKHGYQVTVYERRPDYRKAENY
                     GGRSINLAISERGWKGLAGVGLEEMVREIAIPMPGRMIHDVQGNLNFQPYGKPGEAIN
                     SVSRGGLNVM"
     gene            455..1021
                     /locus_tag="PLM90_07820"
     CDS             455..1021
                     /locus_tag="PLM90_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HMZ89318.1"
                     /translation="MHTRITLTIVCAGLFALSACTKSADCPEPEVKAEDPVQRGQFMV
                     TVGGCNDCHTPKNMTPQGPVPDMSRMLSGHPSGEPVAPYDKNTVGPWVLLSPGLTCAV
                     GPWGTSFAANLTPSESGIGSWSEEQFVKALQEGKHMGMDNTRPIMPPMPWQEIGKFDV
                     NDLKAIYAYLKSIPPVDNVVPAYMPPVQ"
     gene            complement(1090..2361)
                     /gene="kynU"
                     /locus_tag="PLM90_07825"
     CDS             complement(1090..2361)
                     /gene="kynU"
                     /locus_tag="PLM90_07825"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HMZ89319.1"
                     /translation="MSVTFTADKQLAAQLDAADPLKDFRQRFYIPSQDGKEQIYFCGN
                     SLGLQPKSTRSFIEQELIDWQHLGVEGHLHGKNPWFYYHHFLAESTARIVGANPLEVV
                     VMNSLTVNLNLLMISFYRPTKQRYKIIMEYMAFPSDQYAVENQVKFHGFDPFDAIIEL
                     MPREGEAALRTEDILATIEQHKDSIALIMLGGVNYYTGQLFDLSTITAFAKKCSSEIV
                     VGYDLAHAAGNVMLSLHDWGVDFATWCSYKYLNSGPGGTSGVFIHEKHAQDNSLPRLS
                     GWWGNEENTRFKMQKGFFPQPGAAGWQMSNAQILPMAAHRASLEIFDAAGMDNLVQKS
                     KLLTGYLEYLLLSGARKDFKIITPADHAQRGCQLSIVMEHNGKAVFDKLTENGIIADW
                     REPDVIRVAPVPLYNTFEDVWRFADIFHSAL"
     gene            complement(2415..3428)
                     /locus_tag="PLM90_07830"
     CDS             complement(2415..3428)
                     /locus_tag="PLM90_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ89320.1"
                     /translation="MLKIDIHTHIIPEHLPKWSEKFLDNRYIHLDHYCPGCAKMMQGD
                     KFFREVKSNCWDGNVRIEECDATNVNVQVLSAIPVMFHYWEKNAAYALETSRFQNDFI
                     AEVVSRHPTRFIGLGTVPLQDPQLSIEEMERCVKELGLAGIEIGSHVNDWNLNAEELF
                     PFFEAAEKLGAAIFVHPWDMMGKDKMNKYWLPWLVGMPAETSLAICSMIFGGVFEKLP
                     NLKVAFAHGGGSFPSTIGRIEHGFNVRPDLVAVDNKVNPRTYLNKFYLDTLVHDDQML
                     NYLLDLFGPAQLALGSDYPFPLGEHHPGKLIESMPYDDSIKARLLHGTALEWLGLEKG
                     QFE"
     gene            complement(3480..3839)
                     /locus_tag="PLM90_07835"
     CDS             complement(3480..3839)
                     /locus_tag="PLM90_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ89321.1"
                     /translation="MTKQILKAFYTVYNALGYGFLEKVYENALKIELEQSGLQVLQQE
                     SISVFYDNAKVGYYVADMIVGNYDIVENKACSALNSDHEAQLLNYLKATKIEIGLLLN
                     FGLKPEFKIKIFENKYK"
     gene            complement(3928..4446)
                     /locus_tag="PLM90_07840"
     CDS             complement(3928..4446)
                     /locus_tag="PLM90_07840"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HMZ89322.1"
                     /translation="MAIAGPLNLKQWIDDNRHLLKPPVGNQQIYKMNEDFIVMVVGGP
                     NSRKDFHFEDGEELFYQLEGDITVAIQEDGKRRDIVIKEGEMFLLPPNVPHSPMRPAN
                     TVGLVIERYRTEKEFDSCMWFCEKCNHKLHEETFPLQDIVNQLPQIMGKFYADLDLRT
                     CKSCGHVMEPPK"
     gene            4502..5293
                     /locus_tag="PLM90_07845"
     CDS             4502..5293
                     /locus_tag="PLM90_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ89323.1"
                     /translation="MNIQLYPYTAMVCGSTQGIGKACAFELASMGARIVLVARDADKL
                     NQVKQLLPNPHGVEHESIIADFSNPEQVRAAMHAYFSSGEKTVNILVNNTGGPASGKA
                     SDAETEQFLAAFQQHLICNHILATACIPGMKNSGYGRIINIISTSVKAPLPNLGVSNT
                     IRAAVANWSKTLANELGAFGITVNNVLPGATKTERLTGIISANAARTGNAIDAVEHKM
                     IEEIPMKRFGAPEEIANAVAFLSSPAAAYINGINLPVDGGRTASL"
     gene            complement(5317..6324)
                     /locus_tag="PLM90_07850"
     CDS             complement(5317..6324)
                     /locus_tag="PLM90_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89324.1"
                     /translation="MNRKNFLRGLSLAGVGLTLPSSSVFAKPQKNEITGGCILIPTET
                     AGPYPLDLSENAFYFRSDVREIQTGTQLNLKIKVIGNSDCMPMQNVRVNIWHCNKDGL
                     YSGYDNASNPGQAVFTYLRGYQIADANGEVNFITILPGWYNGRVCHIHFRVYVSTAYA
                     AISQLTFDHNTVNAIYAESPDLYTKGADPLTPETDGIFADGYAYQLATLTVNPDTGGY
                     DSYLEVTVEGQGTTGIGWQENVNAQHFSLQQNFPNPATEQTTIPFVLKTASSVQIELY
                     NINAQKIKTIDLGDLPPGNHESQINFEALSLPKANYIYQLVVQNDAGRFTDHKLMTVA
                     K"
     gene            complement(6442..9735)
                     /locus_tag="PLM90_07855"
     CDS             complement(6442..9735)
                     /locus_tag="PLM90_07855"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HMZ89325.1"
                     /translation="MASKKKPTTGGLSFERYFTQDGVSPYDQFTYELRSSVIRNTTGD
                     AVFEMHNVEVPQDWSQVATDILAQKYFRKAGVPQADGSTGSETSIKQVAHRLADCWRQ
                     WGEQYGYFASSKDGQIFYEELAYMILAQMGAPNSPQWFNTGLFSAYGIKGKPQGHYYV
                     DPDSGELKKSTSAYERPQPHACFILSVDDDLVNQGGIMDLWTREARIFKYGSGVGTNY
                     SKIRGEGEKLSGGGTSSGLLSFLKIGDAAAGAIKSGGTTRRAAKMVCLDLDHPEIESF
                     VEWKVKEEDKVAALIAAGYSSDYEGEAYRTVAGQNSNNSVRIPNSFFKVLEEEGQWEL
                     IGRTNGKVFKTVSARKLWDKIAYAAWRCADPGVQYDTTINEWHTCPKSGRINASNPCS
                     EYMFLDNTACNLASLNLRKFYDDDKLVFNVDAFEHACRVWTVVLEISVLMAQFPSPEV
                     AQLSYEFRTLGLGFANLGSLLMVSGIPYDSDKARSIAGAISAIMTGISYKTSAEMAGA
                     LGAFKGYELNKEDMMRVMRNHRYAAYNAADNYEGLEIKPMGIDQALCPEYLLKAACDA
                     WDEALQLGDKFGYRNAQATVIAPTGTIGLLMDCDTTGVEPDFALVKFKKLSGGGYFKI
                     INQSIPLALKNLGYKDESIESIVNYAKGTGTLKGAPSINYDTLKQKGFTETEIDHVDK
                     VMASAFEISFAFNVFTLGAETLERLGFSAEHYSEPGFNLLRALGFTREEIEAANDHIC
                     GTMTIEGAPYLQEKHYSVFDCANKCGKIGERYIHQLGHVRMMGAVQPFISGAISKTIN
                     LPHEATVEDIEECYMLSWQMGLKANALYRDGSKLSQPLSTKSDSKEGSDEKVKHEWTS
                     EEVLEAARSIIMQSTDTIFKRQLSRVVERKKLPAKRDGFTQKSKIGGQTIFVRTGQYE
                     DGTLGEIFIDMHKEGASYRSLLNCFAIAVSIGLQYGVPLEEFTDKFTFTRFEPSGPVD
                     HANIKFSTSIVDYVFRLLAFEYLDRTDLVHVAPEEMSQFEKNPQDISTETTGNYPVTE
                     PVAEKQPKSDSANRKASAAKYQMANAQEAANQSLMGDAPPCPKCGHTTIRNGTCYKCL
                     NCGESLGCS"
     gene            complement(10306..11679)
                     /locus_tag="PLM90_07860"
     CDS             complement(10306..11679)
                     /locus_tag="PLM90_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89326.1"
                     /translation="MKGLIPALLVGLFSTALQGQILTGVTLEGEIYPAHEDLITDPGF
                     FILDCDNDSLGALPLVDLGTGYYMGYQGGLFPGGSNNDPNPHFNNGKNISKAIKPLND
                     LGLVDYLTGEVSLIGIGPSVASDPYNEWKETQNDIDWPGVNPCLNVKGNFIGGKSVAD
                     MLDIDGTYWASFLTGLASKNIDPLQVQIAWMLLVSSTDSNDVNYYVDTVTNQYIQVLH
                     NLQTLCPNLQQVFISGIHYTGYTSPEHKRYDYLIEPYGYWSNLVVKNVITLQINGDSR
                     LTYSGLGKVAPFITWGPYFWADGITPRAYDGLSWPCDNFRDDTIGGGFHLKDTFKFKE
                     ADLIDSFFINNPISKIWYKNAAAWSACGTGRMIDKDDEMYESVSLHCYPNPAHDVIQI
                     PVDENLQGESTLMMYDQYGSLVCVQHFDARTTLTCEVQITNLPSGIYYVLMRDHKQTI
                     AGRFVKT"
     gene            11854..12567
                     /locus_tag="PLM90_07865"
     CDS             11854..12567
                     /locus_tag="PLM90_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ89327.1"
                     /translation="MKKIIHTAISRGHANHGWLDTYHTFSFGHYYDPDRVHFGALRVL
                     NDDTVSGGMGFGKHPHDNMEIISIPLEGDLRHQDSMGNVAVIKQGDIQVMSAGTGIFH
                     SEQNAHADKQVKFLQIWVIPNKQQVAPRYDQITLDPAAGINTLTQIVSPNADDEGVWI
                     HQNAWFHIGTFDKGTTTNYTQHDATNGTYFFVLEGSFEINGEKLDRRDGMGVTELSAH
                     QIKALSEGSRILIMEVEMI"
     gene            12848..13393
                     /locus_tag="PLM90_07870"
     CDS             12848..13393
                     /locus_tag="PLM90_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ89328.1"
                     /translation="MEATATKTKWVVDPTHSSIGFSVKHMMMTTVRGEFEKYAIEAET
                     EGDDFMTAAITFTAETASVNTRNEQRDGHLKSDDFFNAEKFPVISFVPTKFERVDNDD
                     MYTLYGDLTMRDVTKNIKLDVEFGGVMKNPWGSTVAGFIINGKINRKDWNLNWNAALE
                     AGGVLVSEDVRIHIELQLVAQ"
     gene            13497..>14304
                     /locus_tag="PLM90_07875"
     CDS             13497..>14304
                     /locus_tag="PLM90_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89329.1"
                     /translation="MKSLTGILFLFCIHQRACAQLQLQWNTVNTEISESNPIVYAWQR
                     EDLSTHILCTDLGNNFLYNNVISISGDITIGQQLFYPDSLRMLSFKGLAYDNLNNFYV
                     AGMADLGLGGTHEVVAKYAPDLTLKWEYFSPDSVSLIKNAIHRSGDTTIVVSVSSVGM
                     LFTSLHNSGQQVETNFTYNNKYFEFPDLVAYPQNRIVMGFVSDSSILVYVYNGHGSAF
                     NDETIEIEGFTGLLPMSIAYDDSANYYFQLLGYDDEDSLSKIILQVNASAS"
BASE COUNT         4102 a         3057 c         2913 g         4232 t
ORIGIN      
        1 atcatcacat tcaaaccgcc tcgcgatacc gaattaattg cttccccggg ttttccataa
       61 ggctggaagt tcaagttccc ttgcacatca tgaatcatgc gccccggcat cggtattgca
      121 atctctctta ccatttcttc aagtccaacc cctgcaagcc ccttccaccc gcgttcactt
      181 atagccaggt tgatggatcg tccgccataa ttttcagcct tcctgtaatc ggggcgacgt
      241 tcgtacaccg ttacctgata accatgtttt ttgagataca tactcagaag cgagcctacc
      301 agccccgcac ccataatggc aatatttttt acttgcatag cttacttgtg gaagtaacaa
      361 aggtaaaagg atttgatttt tacgacaggt gatttttatc agagttcacc aacataaata
      421 ttttacctaa ctttcaggtt aacaaccaat tatcatgcat actcgaatta ccctaaccat
      481 cgtgtgcgca ggactatttg ccctgtctgc atgcacaaaa tctgccgact gtccggaacc
      541 ggaagtaaag gcagaagatc ctgttcaacg tggacaattt atggtaactg ttggcggatg
      601 taacgattgc catacaccca aaaacatgac acctcaagga ccggttcctg atatgtcgcg
      661 catgcttagc gggcatcctt cgggagaacc tgtagcgcct tacgacaaaa acacagttgg
      721 cccttgggta ttattgtcgc caggattaac atgcgccgtt ggtccatggg gaaccagctt
      781 tgctgccaac ctcacaccat cagaaagtgg aatcggctcg tggagtgaag agcagtttgt
      841 aaaagcctta caggaaggaa agcacatggg aatggataat acccgcccca ttatgcctcc
      901 catgccttgg caggaaattg gcaaatttga tgttaatgac ttaaaggcaa tttatgctta
      961 tctgaaatcc attcctccgg tagacaatgt agtaccggcg tatatgcctc cggttcaata
     1021 agctgaagtc ataaaaaaaa gctcatcaat caagttggtg agcttttttg ttttaaagag
     1081 gtcaataatt tataacgccg aatgaaagat atctgcaaaa cgccatacgt cttcaaatgt
     1141 attatacaat ggcaccggag caacgcgtat tacatccggt tctcgccaat cggcaatgat
     1201 gccattttca gtcagtttgt cgaataccgc ttttccatta tgctccatga ctattgataa
     1261 ttgacaaccg cgttgtgcat gatctgcagg cgttattatt ttaaaatctt tgcgtgcacc
     1321 ggacaacagt aaatattcta aatatccggt cagcaattta cttttttgta ccaggttgtc
     1381 cattccggct gcatcaaata tttcgagtga agctcgatgg gctgccatag gcagaatttg
     1441 tgcattactc atttgccatc ctgctgcacc cggctgtggg aaaaaacctt tttgcatttt
     1501 aaagcgcgta ttttcttcat taccccacca tccgcttaat ctaggcaaag aattgtcttg
     1561 cgcatgtttc tcatgaataa aaacaccact tgttcctccc ggaccgctat ttaaatattt
     1621 ataactacac catgttgcaa aatctacacc ccaatcatgt aaactaagca ttacatttcc
     1681 ggcggcatgt gccaaatcgt atccaacaac tatttcgcta ctgcattttt ttgcaaatgc
     1741 agtaattgtt gacaaatcga ataactgacc cgtataataa ttcacaccac ccaacataat
     1801 aagtgcaata ctgtctttat gttgttcaat ggttgccaaa atatcttcag tgcgcaacgc
     1861 tgcttcaccc tcgcgcggca ttagttcaat aattgcatcg aacggatcaa agccatgaaa
     1921 tttgacctga ttttctaccg catactgatc gcttggaaag gccatgtatt ccatgatgat
     1981 tttatatcgc tgttttgtcg ggcgataaaa acttatcatc aataaattga gattcacggt
     2041 gagtgaattc atcacaacaa cttcaagcgg atttgctcca acaattcttg ccgtgctttc
     2101 tgcgaggaaa tgatgataat aaaaccatgg attttttcca tgtaaatgac cttcaacacc
     2161 aagatgttgc caatcgatca attcctgttc aataaaactg cgtgttgatt ttggttggag
     2221 tccgagcgag ttaccacaaa aataaatctg ctcttttcca tcttgtgatg gtatataaaa
     2281 gcgttgcctg aagtccttta acgggtctgc agcatctaac tgcgcagcca attgcttgtc
     2341 ggcggtaaat gtaactgaca tgccgcaaag gtaggcagaa ttgtcggagc tatagaaaac
     2401 ggcgatgttt caatttactc gaattgccct ttttcaagtc cgagccattc caatgccgtt
     2461 ccatgcagta atcgtgcttt gatagaatca tcgtaaggca tgctttctat caatttaccg
     2521 ggatgatgtt cgccgagtgg gaagggataa tcgctaccga gtgctaattg tgcggggccg
     2581 aacagatcga ggagataatt cagcatttgg tcatcatgca ccagggtatc gagataaaac
     2641 ttattgagat atgttcttgg attcacctta ttatcaactg ccaccaaatc aggtcttaca
     2701 ttaaatccat gttcaatgcg accgatagta gaaggaaagc tgccgccgcc gtgagcgaat
     2761 gctactttta aatttggcaa tttttcgaat acaccaccga atatcattga gcaaatagcc
     2821 agtgatgttt cggcaggcat acctaccaac catgggagcc agtatttatt cattttatcc
     2881 tttcccatca tatcccatgg atgaacaaaa attgcagcac ctaatttttc cgcagcttcg
     2941 aaaaatggaa ataattcttc tgcatttaaa ttccagtcgt tcacatgcga gccaatttca
     3001 attcctgcaa gtccgagctc ttttacacag cgttccattt cctcaatact caattgcgga
     3061 tcttgtaaag gcaccgtacc aagtccgatg aatcgcgtag gatgacgaga aacaacctct
     3121 gcaataaagt cgttttgaaa acgcgatgtt tcaagtgcat atgccgcatt cttttcccaa
     3181 taatgaaaca ttaccggaat tgcacttagc acttgaacat ttacatttgt tgcatcacat
     3241 tcttctatgc ggacattgcc atcccaacaa ttacttttta cttcacgaaa aaatttatcc
     3301 ccctgcatca ttttcgcaca acccggacaa taatgatcta aatggatata tctgttatcg
     3361 agaaattttt cactccactt cggaagatgc tcgggaatga tatgggtgtg gatgtctatt
     3421 ttaagcataa tttttgaaaa ggaacactgg ttgaacaggt taaacagatc gggacggatt
     3481 tatttatact tgttttcgaa aatttttatt ttgaattcgg gttttaatcc gaaatttaat
     3541 aataagccaa tctcaatttt tgtagctttt aaatagttta gtaattgcgc ttcgtggtct
     3601 gagttaagcg cgctgcaggc tttattttca acaatatcgt aatttccaac aatcatatca
     3661 gctacataat aaccaacttt agcattatca tagaatacac taattgattc ctgttgcaaa
     3721 acctgtaatc ctgattgctc aagctcaatt tttagcgcgt tttcgtatac tttttctaga
     3781 aacccataac ctaaagcatt gtatacagta taaaatgcct tgagtatctg ttttgtaatt
     3841 tcggagtgta agtaatccgt ttttatctgt tcaatctgtg ttccttttca acttcacaat
     3901 aatcatcaac caccggaaat ttcagattta cttcggcggt tccattacat gtccgcaaga
     3961 tttacatgtg cgaagatcca aatcagcata aaatttaccc atgatttgcg gtaattgatt
     4021 tacaatatcc tgaagtggaa atgtctcctc atgtaattta tgattgcatt tttcgcagaa
     4081 ccacatacac gaatcaaatt ccttttccgt gcgataacgt tcaatcacca agccaacagt
     4141 atttgcaggt cgcatgggac tgtgcggaac atttggtggg agcaaaaaca tttctccttc
     4201 tttgataact atatctctgc gtttgccatc ttcctgaatt gcaacagtaa tatcgccttc
     4261 caattgatag aacaattctt caccatcttc aaagtggaaa tctttcctcg agtttggtcc
     4321 gccaaccacc ataacgatga agtcttcgtt cattttatag atctgttgat tgcccaccgg
     4381 aggtttgagc agatggcgat tatcgtcgat ccactgtttt aggtttaaag gtccagcaat
     4441 agccatgatt tatctgttta tttctttaat gaattggtag gtggtaaatt tacgcagaat
     4501 catgaatatc caactgtatc catacaccgc catggtatgc ggcagcacac agggtattgg
     4561 caaagcctgt gcgtttgaac tggcatcgat gggtgcccgc attgttttgg tagcccgcga
     4621 tgccgataaa ttgaatcagg taaaacaact gcttcccaat ccacatggcg tagagcacga
     4681 atccattatt gccgatttca gcaatcccga gcaggtgcgc gccgcaatgc atgcatattt
     4741 ttcctcagga gaaaaaacag tcaatatact ggtgaacaat accggcggac ctgcatcagg
     4801 aaaggcttct gatgcggaaa cggagcaatt tttagccgcc tttcagcaac atcttatatg
     4861 caatcatatt ttggcaaccg cctgtattcc gggcatgaaa aatagcggtt atgggcggat
     4921 catcaatatc atcagtactt cagtgaaggc gcctttacca aatctcgggg tatcaaatac
     4981 cattcgcgcc gctgttgcta actggtcgaa aacacttgcc aatgagctgg gagctttcgg
     5041 tattacagta aacaatgtgc tgcccggagc caccaaaacc gaacgtttga ccggtattat
     5101 ttctgccaat gccgcgcgta caggaaatgc catcgatgcc gtggaacata aaatgataga
     5161 agaaataccc atgaaaagat ttggcgcgcc ggaagagata gccaacgccg tagcattttt
     5221 atcttcgcct gctgctgctt atatcaatgg tataaattta cctgttgatg gcgggcggac
     5281 agcaagctta taatatgcaa taatattttc gagcaattat tttgctaccg tcattaattt
     5341 atgatcggta aatcggcctg catcattttg aactaccaat tgatagatgt aattcgcttt
     5401 tggtaatgac aacgcttcga aattaatttg actttcatga tttcccggag gcaaatcgcc
     5461 aaggtctatt gtttttattt tttgtgcgtt gatgttgtat aattctattt gtacggaaga
     5521 agctgttttt aaaacaaaag gaatggttgt ttgctcggtt gccggatttg gaaaattttg
     5581 ttgcaaagaa aaatgttgtg catttacgtt ttcctgccag cctattcccg tagtgccttg
     5641 tccttcaacc gtaacttcca gatatgaatc atatccaccc gtatcaggat ttacagtcaa
     5701 ggttgcaagc tgataagcat acccgtcggc aaaaatgcca tcagtttccg gagttaaagg
     5761 atcggcccct ttggtataca gatcagggct ttcggcgtaa attgcattta ctgtgttgtg
     5821 atcaaatgta agttgtgaaa ttgccgcata tgctgtgctt acatataccc gaaaatgaat
     5881 atggcaaacg cgaccattat accaaccggg taaaatggta ataaaattta cttcaccatt
     5941 ggcatcggca atttgatatc cgcgcaaata agtaaaaact gcctgtccgg gatttgatgc
     6001 gttatcatat cctgaataaa gaccgtcttt attgcaatgc caaatattta cgcgcacatt
     6061 ttgcatcggc atgcaatcgg aatttccaat tactttaatt ttcaaattca gttgtgttcc
     6121 tgtttgtatt tcacgcacat cgcttctgaa ataaaatgca ttttcagata gatcgagtgg
     6181 atatggaccg gctgtttctg ttgggattaa aatacagccc cctgttattt catttttttg
     6241 cggttttgcg aatactgaag atgacggtag agtaagcccc acaccggcga ggctgaggcc
     6301 cctgaggaag ttttttctgt tcatgatgtc aattaagttg cctgaatatg tatttgccac
     6361 tgtcaccggc gaataccatc atgaaggtac ataaaaaaac cgccatcaca ggaaggcggt
     6421 ttttaatatt tgtttgtccg atcaactgca acccaagctt tcgccacagt tcaaacactt
     6481 ataacaagtg ccgttgcgga tcgtggtatg gccacatttc ggacacggag gtgcatcgcc
     6541 cataagcgat tggttggcag cttcttgtgc attcgccatc tgatattttg ctgctgaggc
     6601 cttgcggttt gcagaatcag attttggttg cttttcagct accggctccg taaccggata
     6661 attgccggtg gtttcggttg aaatgtcttg aggatttttc tcaaactggc tcatttcttc
     6721 cggcgccacg tgtaccaggt ctgtgcgatc gagatattcg aatgcgagca ggcggaacac
     6781 atagtccact atggacgtgc tgaacttgat attagcatga tcaaccgggc cgcttggttc
     6841 aaaacgagtg aacgtgaatt tgtcggtaaa ttcttccagc ggaactccgt attgcaggcc
     6901 tatcgaaaca gcaatggcaa agcagttcag cagtgaacgg tacgatgccc cttccttatg
     6961 catatcgatg aagatctctc ccagggtacc atcttcgtat tggcctgtgc gcacgaaaat
     7021 agtttgccct ccgatcttgg atttttgtgt aaagccgtcg cgttttgccg gtaatttttt
     7081 acgttctacc acacggctca actgacgttt gaaaatggtg tcggtagatt gcatgatgat
     7141 actgcgtgca gcttccaata cttcttcgga agtccactcg tgttttactt tttcatcact
     7201 gccttctttg ctatcgcttt tggttgaaag cggttgcgac aatttagaac catcgcgata
     7261 caatgcattt gccttgaggc ccatttgcca gctcagcata tagcattctt cgatatcttc
     7321 cactgtagct tcatgcggaa ggttaatggt tttagaaatt gcacctgaaa taaatggttg
     7381 cacggcaccc atcatacgaa cgtgacctaa ttgatggata taacgctcgc ctatttttcc
     7441 gcatttattt gcgcaatcga atactgaata atgtttttcc tgcaagtaag gtgcaccttc
     7501 tatggtcata gttccacaga tgtgatcgtt tgcagcttca atttcttcgc gagtaaatcc
     7561 taatgcgcga agcagattaa atcccggctc actataatgt tcagcactga atccaaggcg
     7621 ttcgagtgtt tcggcaccaa gtgtgaatac gttgaaagca aagctgattt cgaaagctga
     7681 agccatcact ttatctacat ggtcgatttc agtttcggta aatccttttt gttttaatgt
     7741 atcgtaatta atgcttggcg cacctttcaa tgttccggta cctttggcat aattcacgat
     7801 agactctatg ctttcatctt tataaccaag atttttcaat gcgagcggaa tcgactgatt
     7861 gataattttg aaatatccgc cacctgataa ttttttgaat ttcaccaatg caaaatccgg
     7921 ttcaactcct gttgtatcgc aatccattaa caagccgata gttccggttg gagcaataac
     7981 ggttgcctga gcgttgcgat atccgaattt atcaccgagt tgcaatgctt catcccatgc
     8041 atcgcatgcc gccttcaaca gatactcagg acaaagtgcc tgatcgatac ccattggttt
     8101 aatttccagt ccttcataat tatcagcagc attataagca gcataacggt gattgcgcat
     8161 tacacgcatc atgtcttctt tattcaactc ataacctttg aaggcaccaa gtgctccggc
     8221 catttcggct gaagtcttgt atgagatacc ggtcatgata gcagaaatag cacctgcgat
     8281 tgagcgggct ttatcgctat cgtaaggaat accggaaacc atcaacagtg aacccaagtt
     8341 ggcaaatccg agacccaatg tgcggaactc ataagagagt tgtgcaactt cagggctagg
     8401 gaactgagcc atcaatactg aaatttcgag tactactgtc cacacacgac aagcgtgttc
     8461 aaatgcatct acattgaaaa cgagtttatc gtcgtcgtag aatttgcgga ggttgagtga
     8521 tgcgaggtta catgctgtat tatcgaggaa catgtattcg ctgcatgggt tagatgcgtt
     8581 gatacgtcct gatttcggac aagtatgcca ttcgttgatg gtagtatcgt attgcacacc
     8641 cggatcggca caacgccatg cagcgtatgc aattttatcc caaagtttac gtgcagatac
     8701 tgttttaaat acttttccat ttgtgcgacc gattaattcc cattgacctt cttcttccaa
     8761 tactttaaag aagctgttag gaatgcgcac agagttattt gagttttgac ctgctactgt
     8821 gcggtatgct tcaccttcat aatcagatga ataacctgca gctatcaaag cagcaacttt
     8881 atcttcttct ttaactttcc actcaacaaa agattcaatt tcagggtgat cgaggtcgag
     8941 acacaccatt ttcgcagcgc gacgtgtagt tccgcctgat ttaatggcac ctgcagcagc
     9001 gtcgccaatt ttcaggaagc tgagcagacc acttgaagta ccgccacctg aaagtttttc
     9061 tccttcgcca cgaatttttg aatagttggt accaacgcct gagccatatt tgaatatacg
     9121 tgcttcacgt gtccagaggt ccatgatacc accctggtta accagatcat catcaacgct
     9181 aaggataaag catgcatgag gctgtggacg ttcgtaggcc gaagtagatt ttttcaattc
     9241 acctgaatcc ggatctacat aatagtgccc ttgaggtttt cctttgatac cataagcgga
     9301 aaacaaaccg gtattaaacc attgtggaga gttaggagca cccatctgcg ccaggatcat
     9361 gtatgccaac tcctcataaa atatctgtcc gtctttagac gacgcgaaat aaccatattg
     9421 ctcaccccat tggcgccaac agtcggccag gcgatgggct acttgtttaa tagaagtctc
     9481 acttcccgtg ctgccgtcag cttgcggcac tcctgctttg cggaaatact tctgtgccaa
     9541 tatatcggta gccacctggc tccagtcttg tggcacttcc acattatgca tttcaaatac
     9601 cgcgtcgccg gtggtatttc tgataactga tgatcgcagt tcataggtga actggtcgta
     9661 aggacttaca ccatcctgag taaagtagcg ctcaaatgat aatccgcccg ttgtaggttt
     9721 ttttttgctg gccatgctga ttaatgcgtt tcggtttgtt ggtaaaaggg gtttcgacag
     9781 tttggttcag gtactgacga aaaaacctcc cggttctctc gagaggtttc acgaaagtat
     9841 gacatgaaac tgtttacaaa caagtaacac ttttccaccc ccttgtgggt aagatgaaaa
     9901 tagtcttgta ccgtgggcgt cgcgttgttc acatatattt ttttttaaag taatttttac
     9961 accgtctgtc cacattgatg ttgaaataaa catcgatttt ggggaaaaaa ttcggtcgaa
    10021 aaatttgaaa ttttctttgc atatgaataa gaaggaatgg ctcaaatcag tggcagagcg
    10081 gcatcgaggg ggaaaattga aaaaaacagc ctttttactc tcgtttacca ccacaaatag
    10141 aaaacacaaa cagattaaaa tttttgatgt gttttaatgc tgtggattga tgtgcattcg
    10201 ttaaaaacaa acgatgaatc tgcacgaaaa ttgcacccaa attaaatttt tggcattaaa
    10261 gaaatttgaa ggaaatgtat aacgattgaa aagaaattgt ttcgtttatg ttttcacgaa
    10321 tcttcctgca attgtttgtt tgtgatctcg cattaacaca taataaattc cggaaggcag
    10381 attagtaatt tgaacttcgc atgttaatgt agtgcgtgca tcaaaatgtt gtacacatac
    10441 aagacttcca tattgatcgt acatcatcag ggttgattca ccttgcaaat tttcatctac
    10501 cggaatttga attacatcat gcgccggatt gggataacaa tgcaaggaaa cagattcata
    10561 catttcatca tctttatcaa tcatgcgtcc ggtgccgcat gccgaccaag ctgctgcatt
    10621 tttataccat attttggaaa taggattatt aataaaaaag gaatctataa gatcggcctc
    10681 tttaaattta aatgtatcct ttaaatgaaa acctccgcca atggtgtcat ccctgaaatt
    10741 atcgcagggc cacgacaagc catcataagc ccgaggggta ataccatcag cccaaaaata
    10801 tggtccccag gtaataaatg gcgctacttt tcccaagcct gaataagtta atctggaatc
    10861 gccatttatt tgaagtgtga ttacattttt tactacaaga ttgctccagt aaccataagg
    10921 ttctatcaga taatcatagc gcttgtgttc gggagatgta tatccggtat aatgtattcc
    10981 tgaaataaaa acctgctgca gattcgggca gagggtttgc aaattatgta atacctgaat
    11041 gtattgattg gttactgtgt cgacataata attgacatca ttcgaatctg tcgacgacac
    11101 cagcaacatc catgcaattt gaacttgaag tgggtcaatg tttttacttg cgagccccgt
    11161 tagaaaactt gcccaatagg tgccgtcaat atccaacata tcggcaacac ttttgccgcc
    11221 gataaaatta cccttcacat ttaaacaagg attaacaccg ggccagtcga tatcattctg
    11281 cgtttctttc cattcattat atggatcact cgcaaccgag gggccgattc caattaaaga
    11341 cacttcgccg gtgagataat ctaccagacc taaatcatta agtggcttga ttgctttcga
    11401 aatgtttttg ccattgttga aatgcggatt cggatcgtta tttgaacctc ccggaaataa
    11461 gccaccctgg taacccatat aatatccggt tcccaaatcc accaaaggca gtgcgccaag
    11521 tgaatcatta tcacaatcga gaataaaaaa acccggatcc gtaatcagat cttcgtgtgc
    11581 cggataaatc tcgccttcga gcgtaacccc tgtaagaatc tgtccttgta atgctgtgga
    11641 aaataaaccg accaacaatg ccggaattaa gcctttcatg ttcatgaggt gtgttgtgtg
    11701 cctgagtgcg aagatagaat tttattcagg taatcagcta aaaacacggc tcctgacaag
    11761 catttctgat gtttttttac cctgaatgaa ctatttgcat tttacctctg tttgctatat
    11821 ttgtatcaac atatatgtat atacatatat tttatgaaaa agatcattca cacagcaatt
    11881 tcaaggggac atgctaatca cggttggctg gatacctacc atacattcag ctttggacat
    11941 tattatgatc cggacagggt gcatttcgga gctttgcgcg tattgaatga cgacacggtt
    12001 tccggcggaa tgggatttgg gaagcatccg cacgacaata tggaaatcat ctctattcct
    12061 ttggaaggcg atttgcgtca tcaggacagc atgggcaacg ttgctgtgat aaaacagggc
    12121 gatatccagg tgatgagtgc cggaacaggc atatttcata gtgagcaaaa tgcgcatgcc
    12181 gataaacagg tcaagttcct gcaaatctgg gtaataccca ataagcagca agtagcacca
    12241 agatatgatc agatcacatt ggatccggca gccggcatca acacgttgac gcaaattgta
    12301 agtccaaatg ccgacgatga aggcgtgtgg attcatcaaa atgcgtggtt tcatattggc
    12361 acattcgata aagggactac aacaaactat acgcaacatg acgccacaaa cggaacatac
    12421 tttttcgtgt tggaaggcag cttcgaaata aatggagaaa aacttgatcg tcgcgatgga
    12481 atgggggtaa ctgaattaag cgcacatcaa atcaaagcat tgagtgaggg aagcagaata
    12541 ttgattatgg aagtggaaat gatatgagag gagagaggag aaaggagaga ggagaaagga
    12601 gaaaggagag aggagagagg agagagcttg cctcgccttt ggcgcgtggg tgagtggtga
    12661 gtggttgaat ggaaactgta atacttgata cctgatacct gatacttgat acctgatacc
    12721 tgatacttga tacctgatac ttgatacctg atacttgata cctgatactt gataccaatt
    12781 tcagatactc atcatcaaag cacaaacaaa attgatatta aaaacaacaa aataaaatca
    12841 aattaaaatg gaagcaacag ctacaaaaac aaaatgggtg gtagacccaa cgcattcgtc
    12901 gatcggattc agcgtaaaac acatgatgat gaccactgta cgtggcgaat ttgaaaaata
    12961 tgctatcgaa gctgaaactg aaggcgacga tttcatgaca gcagctatta catttacagc
    13021 tgaaactgca agtgtgaata cacgcaatga acaacgcgat ggtcacttga agagcgatga
    13081 tttcttcaat gcagaaaaat ttcctgtgat ctcattcgtt ccaacaaaat tcgaacgcgt
    13141 agacaatgat gatatgtata cattatatgg cgatttaacc atgcgtgatg taacaaaaaa
    13201 catcaaactg gatgtagaat ttggcggtgt aatgaaaaat ccttggggca gcactgttgc
    13261 cggatttatc atcaacggta agatcaaccg taaagattgg aatttgaact ggaatgcagc
    13321 tcttgaagca ggtggcgttt tggtgagcga agatgtgcgc attcacattg aacttcaatt
    13381 agttgcgcaa taaataattc ggacttataa aaaagcctct tcgaaagaag gggctttttt
    13441 tatgtattta tcgagcgttt ggttatattg caaagcaaac cacaaccgca gaaaccatga
    13501 aatcattaac aggcatctta tttttgtttt gcattcatca acgtgcatgc gctcaattac
    13561 aattacaatg gaacactgta aacactgaaa tatccgaatc taatccgata gtatatgcct
    13621 ggcaacgcga agatttatct acacatatcc tttgcaccga tctgggaaat aatttcttgt
    13681 ataacaatgt tatctccatt tccggggata taacaattgg acaacaatta ttttatcctg
    13741 actcactgcg tatgctatcc tttaaagggt tggcttatga taacttaaat aatttttatg
    13801 ttgccggcat ggccgattta gggcttggtg ggactcatga agttgtagct aaatatgcac
    13861 ccgacctcac tctaaaatgg gaatatttct cgcccgattc agtctcttta ataaaaaacg
    13921 ccatccatcg ctccggcgat accacaattg ttgtatcggt aagtagtgtc ggtatgctat
    13981 ttacttcatt acataattca gggcagcagg tagaaaccaa ttttacttat aacaataaat
    14041 attttgaatt ccccgatctt gtcgcgtatc cgcaaaatcg aattgttatg ggatttgttt
    14101 ccgacagttc aatattggta tatgtttaca atggacatgg gagtgctttt aatgatgaaa
    14161 caattgaaat tgaaggcttt acgggtttgt tacctatgtc catcgcttat gatgacagtg
    14221 caaattacta ttttcaatta ttaggatatg atgatgaaga ttccctttca aaaataatcc
    14281 tgcaggtgaa tgcatccgct tctt
//
LOCUS       DAOQOU010000074        14212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2199_length_14212_cov_6.971675, whole genome shotgun sequence.
ACCESSION   DAOQOU010000074 DAOQOU010000000
VERSION     DAOQOU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_14212_cov_6.971675"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..506)
                     /locus_tag="PLM90_07880"
     CDS             complement(120..506)
                     /locus_tag="PLM90_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMZ89330.1"
                     /translation="MQRTNYSSGAKWEDIVGYSRAVKIGNQIFVTGTVAVDADNQLVG
                     GDDAYLQTKFILEKIATVLQNAGASLKDVVRTRLFVTDISRWEEYGRAHGEVFATIKP
                     CTTMVEVSKLIAPEYLIEIEVDAVVG"
     gene            complement(663..1598)
                     /locus_tag="PLM90_07885"
     CDS             complement(663..1598)
                     /locus_tag="PLM90_07885"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMZ89331.1"
                     /translation="MQFNQAHSLTDIAALIGAKVLADPGTPVTGINEIHKVTPGDISF
                     VDHPKYYDKALNSAATFVIINKEVECPAGKGLLLSDDPFRDYVALVKHFSPFNPSLQP
                     VAKNAVVGEGTICFPGVYIGNNVIIGKNCVLHPNVVIYDNSIIGDNVIIHANTTIGSD
                     AFYFKKRATYWDKLVSCGRVIIHDDVEIGSNCTIDKGVSGDTIIGRSTKFDNHIHIGH
                     GVVIGEMCLFAAGVMIGGKTIIGNQVTLWGQVGVSKTLTIGNNVTVLAKSGVDKDLAP
                     NKIYWGAPAVESRRAWKQLAAANNMLEIWEKMKRG"
     gene            1694..2785
                     /gene="gcvT"
                     /locus_tag="PLM90_07890"
     CDS             1694..2785
                     /gene="gcvT"
                     /locus_tag="PLM90_07890"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMZ89332.1"
                     /translation="MKQTALTNLHKQLGAKMAEFAGYEMPINYSSQVEEHHTVRNGVG
                     VFDVSHMGEFVLKGPAAAALIQKVTSNDVYALSPGKAQYSCLPNENGGIVDDLLVYHL
                     EDGNYMLVVNASNIEKDWNHISKYNTEGVEMVNISDKTSLLAVQGPKAAEALQSLTDV
                     DLKAIPYYNFTKHRFAGVDNVIISATGYTGAGGFEIYFPAEHSLEIWNKVFEAGAAFG
                     IKPIGLAARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKLKAANNEFVGKDKIVAG
                     KEAGLTRKLVGFEMIDRGIPRHDYPIVNAEGAVIGKVTSGTQSPSLNKAIGLGYVENA
                     YSAVDSEIFIDIRQKPVKAKVVKIPFLQQ"
     gene            2816..3544
                     /locus_tag="PLM90_07895"
     CDS             2816..3544
                     /locus_tag="PLM90_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HMZ89333.1"
                     /translation="MPNTQEDNRPIIEVCLTPALWYQHDPKDKNVVVIDIIRATSTIT
                     SALHHGVAKMVPVQTVEEANAYLEKGFLVAGERDGYKIPHFHFGNSPFEFMGDDVKGK
                     TIIHTTTNGTRCVLMSKGAHKIVAGSFLNQEAIFNWIRKENRNTILFCAGWKDHYVMD
                     DALFAGAVVRELADDFRIIDDSTLSAKLLYRYVRENMLRTIKQATHFKRLQRLGIEQD
                     IKFCMSNPHFDVVPVFDGEGFVKG"
     gene            4249..4713
                     /locus_tag="PLM90_07900"
     CDS             4249..4713
                     /locus_tag="PLM90_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ89334.1"
                     /translation="MKELDHNKLKQINNSALLALCILLILGGGLVWEVFTSSSKIFSY
                     EEQTVLGGCGVTNNFVYETGNSPEGELLFQVKCQRCHLVDRKMTGPALMGVRNRWADS
                     TDLYSWIKNSQKFLETGDKYAIDLYKAYGTIMPPFPELSDEQIAELLIYVSP"
     gene            complement(4971..5690)
                     /locus_tag="PLM90_07905"
     CDS             complement(4971..5690)
                     /locus_tag="PLM90_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ89335.1"
                     /translation="MSYRCLIVDDESLALRLMEDHVSKVPGLEIVGKCRHALEALQVL
                     QREEIDILFLDIQMPDLTGVELIKILKHKPAIILTTAYAEYALEGYQLEVVDYLLKPI
                     ALDRFLQAVRKAQEWVDLRKQAPSVPQTTKQDHFFVKSDYKQVRINYADVTHVEGLRE
                     YVSIYAGGKRIVTLETMKNMEQLLPSDLFMRVHKSYIVNTSRIKSINGNQIELGEIRI
                     PLGKVFRAQVLGRLGIISSEG"
     gene            complement(5687..6709)
                     /locus_tag="PLM90_07910"
     CDS             complement(5687..6709)
                     /locus_tag="PLM90_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ89336.1"
                     /translation="MMNQKWYQRIWLLHAAFWSGYLMLLTLIFARFLPIDQAFLRNLV
                     NGCAMAPMVYINLFILVPKLLLKKKFRFYTLCLLGLLIIITPLRLWLDDQFTFNTHNP
                     FPQDSIAHIATIIFSSILMLGVTTTLILFQDWYEKSQMAAAFEKLQLEAELKFLKAQI
                     NPHFLFNALNNIYTLSYLDGKAAAPHIMQLSGLMRYMLYESDEHLVSLSKELTYISHY
                     LELQQLKNGKSQNVTWRVEGETGGVLIAPLILIPFFENAFKHGNAMDDQSGYIHGSLI
                     IGREKMIFHLENSIEKTHKRKDEVGGVGLENVRKRLQMLYHEKHSLSVTMAEYTFTIQ
                     LTLMLI"
     gene            6910..7743
                     /locus_tag="PLM90_07915"
     CDS             6910..7743
                     /locus_tag="PLM90_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89337.1"
                     /translation="MKKHLILLIASLTAVMLAFTACDRSEEEILTEPDPTSAKTSAEA
                     TKTFDDVAMMTEEATVEYMDNPDYDTYTFGPCVTVLHDSINTILTFDLGTTGCVGLDG
                     QMRKGKIIVDYNGVPREPGCTLSITLEDFSVDGYSVSGTLTYQAVDYNTSGDLEIAYA
                     VDNGIYTEPDGDVVTFECERTVTWTEGAGSEDLYDDVFTTNGAFTATLNGDAYSGATV
                     NDIVFKSACWADGIYYPVSGTWQLNFPDGINRVIDYGNGDCDKTVVITVGLRDYTYQL
                     P"
     gene            7757..8521
                     /locus_tag="PLM90_07920"
     CDS             7757..8521
                     /locus_tag="PLM90_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ89338.1"
                     /translation="MREWTDFGFITLWVLSFSVLALRYSLFAGLAFYIMQVVVIRKMV
                     SKRLRQEKIDSKYVFIDIGRSCLSFFIFGAMMAATMIAHQHGYTKLYGRWSDQSYWYA
                     FGSFLLLLFIHDTYFYWMHRLLHTKLLYRYIHKHHHRPTPPTPFTSFAFHPLEAIVEF
                     VFIIPAVMILPLHPIVLFAFANVMTAMNVWGHLGFELFPEKYLQGMVGRIMNSTTHHD
                     MHHQYNKCNYGLYFNFWDSIMHTNHKQYRRLQKREK"
     gene            complement(8541..9842)
                     /locus_tag="PLM90_07925"
     CDS             complement(8541..9842)
                     /locus_tag="PLM90_07925"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ89339.1"
                     /translation="MNPLHNRAFQYFLSMRVFITFAFLIQYVVAGWEIYSITKDPFSL
                     GLIGLAEAIPAISIALYAGHLADISNKRDLLIGALVGMLLSSLGLTVFTSAFMREINS
                     DHTTVMLMYLFIFTTGLARGFYGPTATAIIAQIVIPRKKSFPLKHTLDAIDEEEMRDG
                     ESAKIHSTTLVKASTTNSTVWQMSAIIGPALGGFLYGWIGMTGSMIAVLSCIILGIAM
                     LSQVPSTPPQPSQGEPILERLKAGIRFVFDNKIILNALSLDMFSVLFGGAVALLPVFA
                     NDILKCGPQGLGILRAMPSLGAVITISYISWKKIPGKVGTTLLWAVAGFGVTIILFGL
                     SKNFYLSLFLLFLNGAFDSVSVVIRANIIQLLTPDNMRGRVSAVNTMFIGSSNELGAF
                     ESGLAARLLGTVPSVVFGGTITLCIVGFTSLRAKALKAFEY"
     gene            complement(10134..10469)
                     /locus_tag="PLM90_07930"
     CDS             complement(10134..10469)
                     /locus_tag="PLM90_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HMZ89340.1"
                     /translation="MTIREAQETIDKWITTTGVRYFNELTNMAMLTEEVGEVARIIAR
                     KYGEQSFKESDKDKLLSDELADVLFVLICIANQTGVDLTEALEKNLDKKNVRDVERHK
                     NNDKLRGES"
     gene            complement(10480..11109)
                     /locus_tag="PLM90_07935"
     CDS             complement(10480..11109)
                     /locus_tag="PLM90_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89341.1"
                     /translation="MKKIASKYFEVILIALTVLFLFLKPNLTNFGERISMLFFGTLSF
                     YYLASGILVFLDKNRVGRIMRLMYLFGLWSVSIMVLAIMVRIMLIQMDKALLITAISA
                     SLGLIAYITLYYRRLEGEDREALLYLIQPLFIRVVTSMLIGVAFLIASNYAVYSMFGT
                     HKRDPRYVEKIVSAYENPKDTTIVNDYKRYDEEINKVEEPSPSTNTEQP"
     gene            complement(11114..11566)
                     /gene="dtd"
                     /locus_tag="PLM90_07940"
     CDS             complement(11114..11566)
                     /gene="dtd"
                     /locus_tag="PLM90_07940"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMZ89342.1"
                     /translation="MRAVIQRVNSASVNIEGNCHAEIGKGFLVLLGVEEADGSEDVEW
                     LAQKIAGLRVFADEQGLMNINVQDAGGDILVVSQFTLFASTKKGNRPSFIRSAKPDIA
                     IPLYESFNKTLSTLIGKQIYTGIFGADMQVELVNDGPVTILMDTKNKE"
     gene            complement(11571..12494)
                     /gene="rsgA"
                     /locus_tag="PLM90_07945"
     CDS             complement(11571..12494)
                     /gene="rsgA"
                     /locus_tag="PLM90_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMZ89343.1"
                     /translation="MQGLVIQSTGSFYDVQINGQQMVTCRLKGRLRLDDKKQTNPVAV
                     GDLVEVEQDEKLNYQIAEVHPRRNYIIRSDTHRKHIKQVIAANIDQTVVVASLRKPRT
                     AYGFIDRVLLTSEVYDIPAVVIFNKHDLYDDKDNALLEELMYGYKDAGYDCFSTSVTN
                     KEHVEEIYAVFHNKTSLITGPSGAGKSSLVNMIEPDLNLRIGEISKYTEKGQHTTTFA
                     RMHHLPHGGAVIDTPGIKEFGLVDIDAEEVSHFFPEMRNRIGTCRFNNCLHINEDGCA
                     IKDMLENGELSVQRYTSYFNFYHEIKEAQKW"
     gene            complement(12523..13260)
                     /locus_tag="PLM90_07950"
     CDS             complement(12523..13260)
                     /locus_tag="PLM90_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HMZ89344.1"
                     /translation="MTTDMRSGLKKFAVLIDPDEVGPSELKRLVELGLENHVDYFFVG
                     GSLVIGDAMDMTIRFLKEHCPIPVVIFPGSIMQVNPHADALLFLSLISGRNPELLIGN
                     QVLAAPYVKKSGMEAIPTGYILLDGGAPTTVSYISNTTPIPSDKPEIAACTAMAGELL
                     GMQLMYMDAGSGAKKPIPADVIHAVRKSTDVPIIVGGGIKTPEKAYEDCNAGADIIVV
                     GNAIEKDTSLIHDIAFAIHSASVTSAG"
     gene            complement(13412..>14212)
                     /locus_tag="PLM90_07955"
     CDS             complement(13412..>14212)
                     /locus_tag="PLM90_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89345.1"
                     /translation="DDEDSLSKIILQVNASASLNWMKSTALHKNDLYATLDAKLYVDE
                     DNTLIASTVTQTNDDSVYINMQRYDVAGTIINQRKLNASEWLDPDLSATSAYRASTDM
                     LYVYFPRMQSPDYAGIISMSCNSDWMHVDTLDVAFSEALAQCYAFTDEEDILTLVNLK
                     TDVGPLRNIEVSQFKDTYVGIHQIENPSPIEIFPNPCADYIICALKGQDVNVTSLVIT
                     DLAGRKLIELEDIYSDRVSINTSQLVPGIYQLTIIGSAFRYSETLVKQ"
BASE COUNT         4128 a         2997 c         2896 g         4191 t
ORIGIN      
        1 tatcaagtat caagtatcag gtatcaagta tcaggtatca agtatcaggt aaaaattcag
       61 gactcacgac tcacgactca cgactcacga ttcacgattc acgattcacg attcaccact
      121 caccccacca ccgcatctac ctcaatttca atcaaatatt ccggagcaat taatttactt
      181 acttctacca tagtggtgca tggtttaata gttgcaaata cttcaccatg tgcgcgacca
      241 tattcctccc atcgagaaat atctgtcaca aataatcgtg tgcgcactac atccttcaat
      301 gatgctcccg cattttgcag aacagttgca attttttcga gaataaattt tgtctgcaga
      361 taagcgtcgt cgccaccaac taattgatta tctgcatcaa ctgcaacagt ccccgtaaca
      421 aaaatttgat tgccaatttt taccgcacgg gaataaccaa caatgtcttc ccatttagcg
      481 ccggaggagt agtttgttct ttgcataaat atgtatcaag tatcaggtat caggtatcaa
      541 gtatcaggta tcaggtatca agtatcaggt atcaggtatc aagtattaag ttgaccattc
      601 accactcacc actcaccact caccattcac cattcaccac tcaccactca ccactcaccc
      661 cctcaccccc tcttcatctt ctcccaaatc tccagcatat tattcgctgc agcaagttgt
      721 ttccatgcgc ggcgagattc aactgcagga gcaccccaat atattttatt cggagcgagg
      781 tctttgtcga caccgctttt tgcaagaact gttacattat ttccaatggt gagtgttttg
      841 gatacaccaa cttgtcccca tagtgtaacc tgattgccga taatagtttt tccacctatc
      901 attacccctg cggcaaataa acacatttca ccgattacaa ctccgtgacc gatatgaata
      961 tgattatcga atttcgtgga gcgaccgata atggtatcgc cactcactcc tttatcaatt
     1021 gtacaattag aacctatttc aacatcatcg tgaataatta cacgtccgca gctcactaat
     1081 ttatcccaat aggtagcgcg ttttttaaaa taaaatgcgt cagaacctat tgttgtgttt
     1141 gcatgaataa ttacattatc gccgatgatt gaattatcat agatcactac attcggatgc
     1201 aacacacaat tttttccgat gatcacattg ttgccgatat atacaccggg aaagcaaata
     1261 gtaccttcac ccacaacggc atttttcgca acaggttgta aagagggatt aaatggagaa
     1321 aaatgtttta ccaaagccac ataatctcga aacgggtcgt cgcttaacag caatcccttt
     1381 ccggcaggac attcaacttc tttgttgatg ataacaaaag tagcggccga gttaagggct
     1441 ttatcatagt attttggatg atcaacaaag ctgatatcgc cgggagtaac cttatgaatt
     1501 tcgttgatac ctgtaaccgg tgttccggga tcagccaata ctttcgcgcc tataagtgca
     1561 gcaatatctg tcagtgaatg tgcttggttg aattgcatgg cgcaaaggtc tgaaacaaaa
     1621 tgcataacgc ctcacatcac acagtgaaat cctttagcaa accgtatctt tgcgctcgct
     1681 ttaatcgaaa cacatgaagc agacagcctt aaccaacctt cacaaacaac ttggcgccaa
     1741 aatggccgag tttgcagggt atgagatgcc cattaattat agttcacaag tagaagaaca
     1801 tcataccgtg cgcaacggcg ttggtgtttt cgatgtgagc cacatgggtg agttcgtgct
     1861 gaaaggtcct gcggcagcag ctttgataca aaaggtaacc tcaaatgatg tgtatgcctt
     1921 aagtcccgga aaagcgcaat acagctgcct tccgaacgaa aatggcggta tcgtagacga
     1981 cctgttggta taccacctcg aagacggcaa ttatatgctg gtggtgaatg cttccaacat
     2041 tgaaaaagac tggaatcata tcagcaaata caatacggaa ggtgtggaga tggtgaacat
     2101 ttccgataaa accagtctcc tcgcggtgca gggtccgaaa gcggcggaag cgctgcaatc
     2161 cttgacagac gtggatttga aggcgattcc ttattataat ttcacaaaac atcgctttgc
     2221 gggtgtcgat aatgtaatta tttcagcaac cggctatacc ggcgctggtg gtttcgagat
     2281 ttatttccct gccgaacatt cgctggaaat ctggaataaa gtatttgaag caggagccgc
     2341 ctttggcatc aaacctatag gtctagcagc tcgcgatacc ttgcgtttag aaatgggttt
     2401 ctgcctctat ggcaacgata ttgatgacac aacaagtccg ctggaagcag gtttgggctg
     2461 gattaccaag ctgaaagccg caaacaatga attcgtgggc aaggacaaga ttgtagccgg
     2521 aaaagaggcc ggtttgacgc gtaaacttgt gggttttgag atgatagatc gcggtattcc
     2581 ccgtcacgac tacccaatcg tgaatgcgga aggagcggta attggtaagg taaccagcgg
     2641 aacccagagc cctagcttga ataaggcaat cggattgggt tatgtagaaa atgcatatag
     2701 tgctgttgat tcagaaattt ttatagatat tcgccagaag cccgtgaaag ccaaagtagt
     2761 gaaaatccct ttcctacagc aataattgcg acattaaccg gctattaccc gaattatgcc
     2821 caacacacaa gaagataatc gtcccatcat tgaagtatgc cttaccccgg cgttgtggta
     2881 tcaacacgac ccgaaggata agaatgtggt ggtgatcgat atcattcgag ctacatccac
     2941 catcacttcg gcattgcatc atggcgttgc caaaatggtt cctgtgcaaa ctgtggaaga
     3001 agcaaatgct tatctcgaaa aaggatttct ggttgccggt gaacgcgatg gatataaaat
     3061 accacatttc catttcggaa attctccctt cgaatttatg ggagatgatg tgaaagggaa
     3121 aacaattatt catactacta ctaacggtac tcgttgtgtg ttgatgagta agggtgcgca
     3181 caaaattgtg gccggttcat tcctgaatca ggaggccatc ttcaactgga tacgtaaaga
     3241 aaatcgaaat accattttgt tttgtgcagg atggaaagat cactatgtaa tggatgatgc
     3301 actttttgcc ggcgccgttg tccgcgaact ggctgatgat tttcgcatca tagatgatag
     3361 tacactgagt gctaaattat tataccgcta tgtgcgcgaa aacatgctgc gtaccattaa
     3421 gcaggcaaca cacttcaaac gcctgcaacg cctcggcatt gaacaagaca tcaaattctg
     3481 catgagcaat ccgcactttg atgtagtacc ggtttttgat ggagagggat ttgtgaaagg
     3541 gtgatttaat tagcaaattt gagaattagc agattagcaa atgggggtga ttgcttgcat
     3601 cgggtttatc ctcttagtta gcaagtgtga gagttagcaa gttagcaagt ggtggtgcta
     3661 ttaacgctgg cgcgttaata gggaatgtaa ttatattgtt ttgcaaaata ttaaatccga
     3721 agcgccatta tctcccctct ctatcgacga taggagatgg agaggggccg ggggtgaggc
     3781 gggcgggggg tgaggcgaga attagcaagt tagcaagtgg tggtgctatt aacgctggcg
     3841 cgttaatagg gaatgtaatt atattgtttt gcaaaatatt aaatccgaag cgccattatc
     3901 tcccctctct atcgacgata ggagatggag aggggccggg ggtgaggttg gtttcgcgca
     3961 aagacagcga agaggctaag gcgcaaaagt ctagatacta gatacttgat tctagatact
     4021 gaaagagggt tagcaagcga ggttaattag caagtgtgag aattagcaaa ttagcaagtg
     4081 tgggtgcttt taacgctggc gcgttaatag ggagtgcaat tatttttgca taatccatat
     4141 tacatctgct ataaccattc acaattatgt aatactctgt aaattgcttt tgttggatta
     4201 ttttatcttg ctttatatat ctatatttga ataagtcctc gtcattttat gaaagaattg
     4261 gatcataata aattgaaaca aatcaacaat tctgcattgc tggcattatg tattttattg
     4321 atcctggggg gcggacttgt atgggaggta ttcacatcat ccagtaaaat attttcatat
     4381 gaggagcaaa cggttttagg gggatgtggc gttacaaata attttgttta cgagacggga
     4441 aattcgccgg aaggagaact attgtttcaa gtcaaatgcc agcgttgcca tttagttgac
     4501 aggaaaatga ccggaccggc attaatgggt gtgcgcaaca gatgggctga ttctactgat
     4561 ttgtattcat ggataaaaaa cagtcaaaag tttctggaaa caggcgataa atatgccatt
     4621 gatttatata aagcgtatgg tactatcatg cccccatttc ccgaactctc tgatgagcaa
     4681 atagctgagt tgctcatcta tgtgagccca tgagcacttt gaccaattct tcctgtgtta
     4741 gatgtatttg agttatagca atgagggata atcaatatag ataattgaac tttttctctt
     4801 gggttatttt gcattaccca gccaactctg cgattgaata tgtgttaatg gtgaatagcg
     4861 actcgtgaat taaaccttcg aatcttcaac tcttcaaacc ttcgactctt caaaccttcg
     4921 aatcttcgac tcttcgaatc ttcaactctt caaaccccct tccatttacc tcaaccttca
     4981 gacgatataa tacccaaccg ccccaaaact tgtgcacgaa atactttgcc gagtggaatt
     5041 cttatttcac ccaattctat ctgattgcca tttattgatt ttatgcgcga tgtatttaca
     5101 atataagatt tgtggacacg cataaacaaa tccgagggta ataattgctc catgtttttc
     5161 atggtttcta atgtaactat gcgtttccct ccggcataaa tacttacata ttctcgtagt
     5221 ccctcaacat gcgttacatc tgcataattg atgcgcactt gcttgtaatc tgattttaca
     5281 aaaaaatgat cctgcttggt tgtttgtggt actgatggag cttgttttcg cagatctacc
     5341 cattcttgtg cttttcgaac agcctgaagg aaacgatcca atgcaatggg ttttaataaa
     5401 taatctacaa cctctaattg atatccctcc agcgcatatt cggcatatgc tgtggttaaa
     5461 atgattgccg gtttgtgctt gaggatttta attaattcca caccggtgag gtcaggcatc
     5521 tggatatcga gaaataaaat atcaatttct tcgcgttgta atacctgcaa agcttcaagc
     5581 gcatgtctgc atttgccaac aatttctaat ccgggcacct tcgacacgtg atcttccatc
     5641 aaccgcaatg caagcgattc gtcatcaact atcagacatc tgtaactcat atcaacatta
     5701 acgttaattg aatagtaaaa gtgtattcgg ccatggttac actcaggctg tgtttttcat
     5761 gatatagcat ttgcaatcgc ttgcgcacat tttccaatcc cactccgcca acttcatctt
     5821 tccgcttatg tgttttttct atgctgtttt cgagatggaa tatcattttt tctctgccaa
     5881 tgatcaagct tccatgaata tatcccgatt gatcatccat tgcatttcca tgtttgaatg
     5941 cgttttcgaa aaatggaatt aatatcagtg gcgcaattaa tacgcctcct gtttctcctt
     6001 caacacgcca tgttacattt tgcgatttgc cattctttaa ttgctgtaat tcaagataat
     6061 gactgatata tgtaagctcc ttcgataagg aaacaaggtg ttcatcactt tcataaagca
     6121 tatagcgcat caacccactc aattgcataa tatgtggagc cgcggctttg ccgtcaagat
     6181 aactaagagt atatatatta tttaaggcat taaataagaa gtgcggattt atctgcgcct
     6241 ttaaaaattt caattcagcc tccaattgca gtttttcaaa tgcagcagcc atttgcgatt
     6301 tttcatacca gtcttgaaac aatattaagg tggtggtaac tcccaacata agaatggatg
     6361 aaaaaatgat tgttgctata tgcgcaatgc tatcttgcgg aaatggatta tgggtgttga
     6421 acgtaaattg atcatcaagc cacaagcgca gaggtgtgat gatgataagt aagcccaaca
     6481 agcataacgt gtaaaaccta aacttttttt tgagcagcag ctttggcacc aaaataaaca
     6541 ggttaatgta taccatggga gccattgcgc agccattcac cagattgcgc aggaatgcct
     6601 gatctatggg aaggaatctg gcaaatatca acgtaagcag catcagataa ccactccaaa
     6661 aggcagcatg cagcagccat atacgttgat accacttttg attcatcata caaacttacg
     6721 tgccattggg catttatttg gcagtttatc taccaacgtc acatttattt ctacgaacga
     6781 aaaatcaagc atcgaaataa cgcttgtaga tgaaatattt actgtcattg tgatttattg
     6841 aatacctgtt gattaagttt tttgcacata aattatcgat gcacttttgc ttcatcaaaa
     6901 caacaatata tgaagaaaca cttgatctta cttattgcct cactcactgc tgtgatgctg
     6961 gcatttacgg cctgtgatcg ttcggaggaa gaaatcctta ctgaaccgga tcctacatct
     7021 gcaaaaactt ctgcagaggc aacaaagact tttgatgatg ttgctatgat gaccgaagaa
     7081 gccaccgtcg agtatatgga caaccctgat tacgatactt atactttcgg tccctgcgta
     7141 acagtattgc acgactctat caatacaata ttgactttcg atttgggaac aacaggttgt
     7201 gtgggactcg atggtcaaat gcgaaaagga aaaattattg tcgactacaa cggcgtgccg
     7261 cgtgaaccgg gatgtacctt gtcaatcacg cttgaagatt tttctgttga tggatacagc
     7321 gtaagcggca cgttgacata tcaagctgtt gattataata cctccggtga tcttgaaatc
     7381 gcttatgctg tggataatgg aatttataca gagcctgatg gtgatgtggt gacctttgaa
     7441 tgcgaaagaa ctgttacgtg gactgaaggt gccggaagtg aagatttgta tgatgatgta
     7501 ttcaccacta atggagcatt tactgctacc ttaaatggtg atgcatacag tggcgctacg
     7561 gttaatgata tagtatttaa atctgcatgt tgggctgatg gcatttatta tcccgtttca
     7621 ggcacatggc aattaaactt tcccgatggc ataaatcgcg ttattgatta tggcaacggc
     7681 gattgcgata aaactgtcgt gattactgtc ggattgcgtg attataccta tcagctaccc
     7741 taattgaaat aattccatgc gtgagtggac agatttcgga ttcattaccc tctgggtgct
     7801 gtcgtttagt gtgctggctc tgcgatattc cctattcgca gggctggctt tttatattat
     7861 gcaggttgtt gtgattcgca aaatggtatc taaacgacta aggcaagaga aaattgatag
     7921 caaatacgtg ttcattgata ttggaagatc gtgcctcagt tttttcattt tcggtgctat
     7981 gatggctgcg actatgatag cccatcaaca tggttataca aaattgtatg gcagatggag
     8041 cgatcaatct tattggtatg cattcggctc attccttcta ttgttattta ttcacgacac
     8101 gtatttctat tggatgcata gattactgca tacgaaattg ttatatcgat atatccataa
     8161 acatcatcat cgccctacac cgccaacgcc ttttacgagc tttgcatttc atccgcttga
     8221 agctattgtg gaatttgttt ttattattcc tgctgtcatg attttgccct tacatcctat
     8281 tgtcttgttt gcatttgcaa atgtgatgac agctatgaat gtttgggggc atttgggatt
     8341 tgaattattt cccgaaaaat atttgcaagg tatggtaggt agaataatga attcaaccac
     8401 gcatcacgat atgcatcatc agtataataa atgtaattat ggcttatatt ttaatttctg
     8461 ggacagcatt atgcatacta accacaaaca atatcgccgt ttacagaaaa gggaaaaata
     8521 atttaagaga attatttatt tcagtattcg aaagccttca atgctttcgc gcgcaatgat
     8581 gtaaatccta caatgcacaa ggtaattgtt cctccaaaaa ctactgaggg cactgttcca
     8641 agcaacctgg cggcaagtcc gctttcgaag gcaccaagct cgttactgct gccgatgaac
     8701 atggtgttta ccgcactaac gcgtccgcgc atattatcag gtgtgaggag ctgaatgata
     8761 tttgcccgta tcactacact tacactgtcg aaagcgccat ttagaaataa taaaaacaat
     8821 gacaagtaaa agttcttact aagtccaaac aaaataatag ttacaccaaa gccggcaact
     8881 gcccacaaca atgttgttcc taccttacca ggaatttttt tccatgagat ataacttata
     8941 gttataaccg cacctaatga tggcatagca cgcaaaattc caagaccttg cggaccacat
     9001 tttaaaatat cgttggcaaa caccggtaat agggctactg ccccaccgaa aagcacggaa
     9061 aacatatcca aactcagggc atttagaatt attttgttgt cgaatacaaa acgaatacct
     9121 gcttttaatc tctccaaaat aggttcgcct tgagatggct gtggtggtgt tgatggtact
     9181 tgcgacaaca ttgcaatgcc taatatgatg cacgataata ctgcgatcat agaacccgtc
     9241 atgccaatcc aaccatataa aaacccacca agtgccggtc ctatgatggc gctcatttgc
     9301 cagaccgtag aatttgttgt agatgctttt acaagtgtgg tactgtgaat tttcgccgat
     9361 tcaccatctc gcatctcttc ttcgtcaatt gcgtcaagcg tatgttttag ggggaaggat
     9421 tttttgcgag gaataacaat ctgtgcaatt attgcagtag ccgtcggccc ataaaatcca
     9481 cgtgcaagtc cggtggtgaa aatgaataaa tacatcaaca tgaccgtagt atggtcacta
     9541 ttgatttcac gcataaatgc cgaggtaaaa acggttagtc ccaatgatga tagcaacata
     9601 cctacaagcg cgcctatcaa caaatcgcgc ttgttgctga tatcagccag atggccggcg
     9661 tacagcgcta tacttatcgc aggaatggcc tcagccaaac caatcaaacc taaactgaaa
     9721 ggatctttgg tgatggaata tatttcccat cctgcaacca catattgaat aagaaatgcg
     9781 aatgtgataa acacacgcat gctgaggaaa tattgaaatg cccggttgtg gagtggattc
     9841 acagggtaaa attacgcatc gaaaagaata ttgtgacttt ggatttgttt tggccgtttg
     9901 gcaatgagta gtgtttactc gtgaatcaca taccacactc acgagtcatc actcatcaat
     9961 ttttcacgca gagaccggag gcgcagagag ggtaaagatt cgaaggtttg aagagtcgaa
    10021 gattcgaagg gtggaagaat agaagattcg aaggatggaa gaatagaaga ttcgaaggat
    10081 tatttcacta ttcacgagtc acgaatcacg agtcacgatt cacgtctcac gcctcacgat
    10141 tcaccgcgaa gcttatcgtt atttttatgc cgctcaacgt ccctgacatt tttcttatca
    10201 agattttttt cgagggcttc ggtgaggtct acaccggttt gattggctat gcaaataagc
    10261 acgaatagaa catcggctaa ttcatcagat aatagcttgt ctttatcgct ttccttaaac
    10321 gattgctcgc catattttct ggcaataata cgggccacct cacccacttc ttcagtcagc
    10381 atggccatat tggtaagctc attaaaatag cggacgccgg tagtggtaat ccacttgtcg
    10441 atagtttcct gagcttcacg aatggtcatg gctttatatt tatggttgtt cggtatttgt
    10501 ggatggagaa ggctcttcaa ccttatttat ttcctcatcg taacgtttat agtcgtttac
    10561 tatggtggta tcctttgggt tttcgtatgc gcttacaatt ttttcgacat aacgcggatc
    10621 gcgtttgtgc gttccaaaca tgctatacac agcataatta cttgcaatta aaaatgcaac
    10681 accaattaac atggaagtta ctacacgaat aaataatggt tgtattaaat ataacaatgc
    10741 ttcgcggtct tcaccttcca gtcggcggta atacaaagtg atataagcta ttagtcccag
    10801 tgaagctgaa atagcggtga tcagcaatgc cttatccatt tgtatcagca taatgcgtac
    10861 cataattgcc aataccatga tggaaacaga ccaaagccca aaaagataca tcaatcgcat
    10921 gattcttccc acgcgatttt tatccagaaa aactaatatg cctgaagcga gataataaaa
    10981 tgataatgta ccgaagaaca gcatgctgat gcgctcaccg aaatttgtca aattaggttt
    11041 tagaaaaaga aataataccg taagcgctat cagtattact tcaaaatatt tcgacgcgat
    11101 ttttttcact tttttactct ttattttttg tatccatcag aatggttacc ggtccgtcat
    11161 tcactaactc cacctgcata tcggcaccaa agataccggt gtatatctgt tttccgatga
    11221 gtgtactcaa tgttttattg aatgactcat ataagggaat cgcaatatcg ggcttggcgc
    11281 ttcgtataaa ggatgggcga ttgccttttt ttgtcgatgc gaaaagtgtg aattggctta
    11341 caaccagtat atcaccgccg gcatcctgta cattgatatt catcaaaccc tgctcatctg
    11401 cgaaaacccg cagccccgca atcttctgtg caagccactc aacgtcttct gaaccgtctg
    11461 cttcttcaac gccaagcaat actagaaaac cttttccgat ttctgcatga caattgcctt
    11521 ctatattgac agatgcactg ttaactcttt gtatcactgc tctcataact tcaccatttt
    11581 tgagcttcct tgatctcgtg ataaaaatta aaataactgg tataacgctg aacagataat
    11641 tcgccgtttt ctaacatatc tttaattgca caaccatctt cattaatgtg aagacaatta
    11701 ttgaatctac atgtgcctat gcgatttcgc atttcaggaa aaaagtgact tacttcttct
    11761 gcatcgatat caacaagtcc aaattctttt atacccggtg tatctatcac agcacctcca
    11821 tgtggcaaat ggtgcatacg cgcaaatgtg gtagtatgct gacctttttc tgtgtatttt
    11881 gaaatttccc cgatgcgtaa attcaaatca ggctctatca tatttaccaa tgatgatttt
    11941 ccggcaccgg aaggccctgt aatgagagat gttttattat gaaatacagc atagatttct
    12001 tcaacatgct ctttgtttgt tactgaagtg ctgaagcaat catatcccgc atctttatat
    12061 ccatacatca attcttcaag taatgcattg tctttatcat catagaggtc gtgtttatta
    12121 aaaataacaa cagcaggaat atcgtacact tcacttgtaa gcaatacccg gtcaataaat
    12181 ccgtaagcag ttcgtggttt tcttaaactg gcaacaacaa cagtttgatc gatattcgcc
    12241 gcaattactt gtttgatatg tttgcgatgg gtatcactgc ggataatata atttcttcgc
    12301 ggatgtactt cagctatctg ataatttaat ttttcatcct gctccacttc caccaaatcg
    12361 ccaactgcaa cgggattggt ttgcttctta tcatcaagtc gcaaccttcc tttaaggcga
    12421 caggtcacca tttgttgtcc attgatctga acgtcgtaaa aacttcctgt actttgaata
    12481 acgagaccct gcatcagtat atgtgggtgt ttactcgccg cttcatccgg cagatgtcac
    12541 cgaagctgag tgaatggcaa aagcgatgtc gtgaatgagc gaagtgtctt tttcaatggc
    12601 attgcctaca acaatgatat cggcaccggc attgcaatct tcataagctt tttccggtgt
    12661 tttaatacca ccgccaacaa tgataggtac atcagtgctt tttctaacag cgtgaattac
    12721 atcggcagga attggttttt tcgccccgct gcccgcgtcc atatacatca attgcatacc
    12781 aaggagttca cctgccattg ctgtgcaagc tgcaatttcc ggcttgtctg aaggtatagg
    12841 tgtggtgttg gagatataac ttaccgtagt aggtgctccg ccatctaaga gaatatatcc
    12901 ggtaggaatg gcttccatcc ctgatttttt aacatatggt gccgccaaaa cctggttgcc
    12961 aatgagcaat tcagggtttc tgccgcttat cagactcaaa aaaagcaatg cgtcggcatg
    13021 aggattaact tgcataatgc ttccggggaa tatcactacc ggaatggggc aatgctcctt
    13081 cagaaagcga atggtcatat ccatcgcatc gccaatcacc aagcttccgc ctacaaagaa
    13141 atagtcaaca tgattctcca aacccaactc caccaagcgt ttcagctccg aagggccaac
    13201 ctcgtccggg tcgatgagca cagcgaactt cttcagtcca ctccgcatat cggtggtcat
    13261 ttgatgtaga atagggcgat gcatgatggt gatcggagca aaaatagtga aaaagacgtg
    13321 gaataagcga ctttaaaacc cgctcaatcg gcagtggaaa ggcttgaaaa ggaacctccc
    13381 gcatatggtc agcctaaccg gtgaagggga atcactgttt gaccagcgtc tcactgtatc
    13441 tgaacgcaga gccaataatg gttaactgat atatccccgg aacgagttgt gaggtgttga
    13501 tggaaacacg atctgaataa atatcttcca attcaataag cttccttccg gccaaatctg
    13561 tgatgaccaa cgaagtaacg ttaacatcct gccctttcaa cgcacaaata atataatcag
    13621 cacacggatt tggaaaaatt tcgattggtg atgggttttc aatctgatgg atgcctacat
    13681 atgtatcttt aaattgcgac acttcgatgt ttcgcagcgg acctacgtcg gttttcaggt
    13741 tgactaatgt aagtatatct tcctcatcag taaaagcata gcattgcgca agcgcttcgg
    13801 aaaatgcaac atctaaagta tctacgtgca tccaatcgct attgcaagac atgctaatga
    13861 tgcctgcgta atcaggagat tgcatgcgag gaaaatatac atacaacata tctgtggatg
    13921 cgcggtatgc ggatgtggct gaaagatccg gatctaacca ttcagatgca tttaatttgc
    13981 gctgattaat tattgttcct gcaacatcat atcgctgcat attgatataa acactgtcat
    14041 catttgtttg agttaccgta cttgcaatca gcgtgttgtc ttcatcaaca tacaattttg
    14101 catcaagcgt tgcatacaaa tcatttttat gtaatgcagt tgacttcatc caattcaaag
    14161 aagcggatgc attcacctgc aggattattt ttgaaaggga atcttcatca tc
//
LOCUS       DAOQOU010000075        14021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2241_length_14021_cov_5.149220, whole genome shotgun sequence.
ACCESSION   DAOQOU010000075 DAOQOU010000000
VERSION     DAOQOU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14021
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_14021_cov_5.149220"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2086
                     /locus_tag="PLM90_07960"
     CDS             <1..2086
                     /locus_tag="PLM90_07960"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89346.1"
                     /translation="QVTYQWQQAHGGSVNDGAYSINATSDGGYIVGGYSYSGISGDKT
                     MASFGGEDYWVLKLDVDGNIQWQKAYGGSGLDRLNKVVQITDGGYLVGGYSQSMISGN
                     KTENSYAGTQDVWVLRLDSLGNILWQTCLGGSGIDMLEDVNETADGGCIIGAYSTSGN
                     SGDKTEGSKGVYDYWIIKLNSTGGIMWQNTIGGTGSDYCRGAQQLPDGGYFISGYSGS
                     PISFDKTEACVGPFNNNDAWIMKLDASGNIIWQNVIGGSSNDNLTAAKLTPDNGAILA
                     LYSSSNISGDKSENTIAGVTTDADYWIVKVDAAGNVMWENTIGGIDVDLCYDVTLMSD
                     GGYALAGYSLSPMSGDKTTGVYASSFDYWIVGVDALGNVLWDRNYGTYLDDYCYGITT
                     GADGMLVAAGDAESVTTGDKIVTGNGWKDFWLIKLRNVCIPTDEVCNDIDDDCDGIID
                     EGVVENVYIVDPGITTVCQGTPVNMASVFTAGVPQWYRNGVAIPGAIGIYYTATVSGN
                     YTCVTSSDCGTDTSNVVHITVNKNPKASISAGGPTTFCMGGSVTLTEMPSAGCTYQWM
                     KGASLVAGATSNVYVATTTGNYKCLVTKTATGCFKTSNTIPVNVICKLDGSDNISMSV
                     FPNPTHDLIMLEIPENEIVDVWIYDQSGKLLTSLNAFESGDISVENLPAGTYLVKATN
                     DLHQWNAYFIKQ"
     gene            complement(2166..3419)
                     /locus_tag="PLM90_07965"
     CDS             complement(2166..3419)
                     /locus_tag="PLM90_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89347.1"
                     /translation="MKLKEYGLAAAAILASGDVSNQTAMYQDIDPDINIPAGDPFGWF
                     YSIANIDLNHDGVDDFRFRLEGFTTYTGWDAVGSFIQAAAIPLGSNQVQIASSQYIGW
                     CSIGWNAANFANVANYSNGQMIHANPTAGSFIEPINEEQSHQNLFIQRVKGYFASSWS
                     YFGDCDNPEDFYMFTGGFWFHADSRVIPFKVKDGGNLYTGWIRLSVDEGSLNIEDYAI
                     SMSPEANIVAGSLTGTVTAQAPNPGLVTVATTKANLTWSSVPGAIKYRFRYRPVGATV
                     WITKMTTTTSKLIKSLTCGTAYEWQVQAVYDNTPALNSEWSDVVNFSTSSCRIGDENT
                     YVEDVIVYPIPASNSITIETVSPAETIDAFVELYNLQGSLIKSYTTNSKEFTMDVSDV
                     APGTYMVVINNSNEEPIVQKVIIAR"
     gene            3501..8000
                     /locus_tag="PLM90_07970"
     CDS             3501..8000
                     /locus_tag="PLM90_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HMZ89348.1"
                     /translation="MKLSCANLVVGLFILLVSFLTPTLHLNATSPSLPDGNDLRQLTS
                     ASFTDPANNFMVNIEGNAFNITHKENGWSLDARLTGMSRANATYPIEVMAGNVNNSKI
                     LTFHDAKIDIIYQLTSMGMRQDFVLKEKPAGEGDISLQLAFSSAQIEFSCNGSTLTGS
                     LNEIPVYAYTALKVWDANNAPLAASMQWHEGVLSLILNDDNAVYPITIDPVSITPNAI
                     LTGNMLSSQYGNDIDNAGDVNGDGYDDLIVGSIFYSNGQSSEGAAYIYLGSASGISTT
                     PQIILESNQNSANFGKAVAGAGDINNDGYDDIVIGAYNYDNGQTNEGRAYIYMGSASG
                     ISATPALTLEVNQAQCNFGSSVAAAGDVNNDGFADVIIGAYNYDNGQTNEGRAYVYHG
                     SATGLNATPVSIMESNSAQAYFGWSVGGDMDVNGDGFDDVIVGAYNQSNGQANEGLAY
                     VFHGSASGIITTPAATREMNQVNAFFGESVSNAGDVNNDGYDDVIIGAYLFDNGMSTE
                     GAAFIYHGSPTGISAAPITQLEANIMFAYFGYSVDAAGDINGDGFDDVIVGAYIFEYD
                     QQDEGRAFVFPGSVTGVSDVPDAVMERNEINANFGYAVCGGGDFNNDGFSDVAVSAPK
                     KDFAVTDAGGVFVYHGSNCTPHLLFEDFDGDGYGAFNYPIVSCDATAAGYAVNNDDCV
                     DDNAAQNPTTVWHLDADGDGYYIDDPAGITQCESPGTGYAFAGILGGDDCDDANPAAN
                     PGATEMVNGVDDNCDGIIEPWAWEGAVTLEGNQTSAYFGSAVSSAGDVNNDGYDDIIV
                     AANGWTDVQSDEGAIFIYHGSATGVSPTPNTSIQSNYAFAYFGYSVAAAGDVNGDGYD
                     DIIVGATNYELGQSNEGAAFIYHGSATGINTTPATILQADQADAFFGYDVAGNADLNN
                     DGYDDVIVSAYMFDNGQTNEGRVFIYYGSPSGVNSTPVLTLESDQPNAYFGRSIAING
                     DVNNDGYEDLVIGASHYDNILSGEGRVFVYLGTAAGIDPLQVSYHESNQTLAYLGRSV
                     AMSDVNGDGYDDVIAGAHYYDDTEINEGAIFVFNGSASGVSTTADDIIETNQGDAFLG
                     ISLASLDDINNDGYDDIITGATGFDGAEEDQGKAFQFNGSASGLSPSPEAGVFERAQY
                     KADYGVAVANAGDVNGDGINDILIGADKYDAPSSDEGIAMLFLSCSTQFFADADGDGY
                     GDDATLVNSCNAPLGYVSIGGDCDDDNASVHPGATEICNGIDDNCNGNIDEGFELSIT
                     IAALGATTFCEPGTVTLSASYTGTSIQWKRNGTNIAGATSATYTANQSGTYRAETSNA
                     CGSDLSEGIVVTVNKKPTATITASGATTFCAGGSVTLNVTPVAGCSYQWYNGATAIGG
                     ATGTAYVATTAGSYKCRVTKTATGCYKNSNIIDVTVPCKESLNSELGFSVFPNPANEQ
                     LNVTVSEEIDGVVHITDLAGNIVIATEMHTGNMAIDISILPAGMYMLFILQESKQLET
                     KFIKQ"
     gene            8011..9489
                     /locus_tag="PLM90_07975"
     CDS             8011..9489
                     /locus_tag="PLM90_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89349.1"
                     /translation="MRKLLFTSAFVCLHIAFLHAQDWSLFPADNHSYYRNNDMIYTVT
                     PDSNYVSGDTVFTLFQFSDTPLSASGCTNEIEDYLYAGGSLFLFPYVDYLAKVGDLTV
                     ITFGSGEYQMRFNTMCAKDDTFHLGGNLDPDLYIICNDVRIDNIFGTLDSVREFQVLH
                     DSGYDISLENTFILSKTFGLLSFYDFNQLPYYAIASMQYFEMIGIEGPIGDKGFKPPF
                     WKDYFPLQVSDVLIWDRHIYSHIPMYYIDYHLYYKDSVIARMDYPDSIVFYFDRWQKD
                     TSGIITQLPTYRLRHKRSELINMLEGPTGRFKHIEVTPGCYYFQHNILKSDGLKMLFS
                     GDDTIYSYQYFGGVYEIYDGCFVGETYDVGKSFTLQTDHGLVAGSYSVGDGNDYIVLA
                     GGIINGESFGIVDFETVDIANDLIPTFSLFPNPASEAVNIVLPDNVSGDIRIFNTAGN
                     CIVHAVSNIGKLTLDTSNWPSGIYMVQVVTNADSSSQRLMKF"
     gene            9592..10113
                     /locus_tag="PLM90_07980"
     CDS             9592..10113
                     /locus_tag="PLM90_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89350.1"
                     /translation="METLRVAFVLIACTISSAVNAQWSAEAQLAGSNFLGASLNLNYS
                     LSISANGDHKLTPTIGLGLSSNEWMPTSRMIIHAGLNYSYKRYGIGVEASQFGPLFPK
                     DGVAYDNELIVFPNINYLIVDRDMWYLKASLGAYFAFSELDGIDDKPTPTLSFEGDVI
                     PGAGLSVGIKLFK"
     gene            complement(10174..11460)
                     /locus_tag="PLM90_07985"
     CDS             complement(10174..11460)
                     /locus_tag="PLM90_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ89351.1"
                     /translation="MSFDKLGLIAPILKALQQEGYENPTPIQEQSIPAAIQGKDILGC
                     AQTGTGKTAAFAIPVLQRLHAANNKHPRAIRALILTPTRELAIQIGESFEAYGKYVGL
                     YHTVIFGGVSQHAQTQQLKRGIDILVATPGRLLDLMQQGFISLQHIETFVLDEADRML
                     DMGFIHDVKKVIAKLPAKKQTLFFSATMPKEITALADSLLHHPVKVAVTPVSSTVDLI
                     EQSVYFVSKGNKKQLLHHILKTKDIKSCLVFTRTKHGANKVCKDLVRAGFTAEAIHGN
                     KSQNSRQSALTNFKSGKTQVLVATDIAARGIDVEELTHVINYELPNIPETYVHRIGRT
                     GRAGSSGVAIAFCDEEERAYLRDIQKLIGKTILVHIDHPEYKKEIATPDAPLHAQRNR
                     PRTEKPKQQQTASAGGQKPRSKKPFWKNKKKQSKTA"
     gene            12093..12449
                     /locus_tag="PLM90_07990"
     CDS             12093..12449
                     /locus_tag="PLM90_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ89352.1"
                     /translation="MAYINRKNEVLAMSFDFSKALVYYCKQIEETGHKHIAWQLLRSG
                     TSVCANIFEAQTPESKSDFIHKLKIAAKECQETIYWLLLINETIESDKSEVYLKTAHS
                     IARLLSTIIASTRKSL"
     gene            complement(12558..13094)
                     /gene="dcd"
                     /locus_tag="PLM90_07995"
     CDS             complement(12558..13094)
                     /gene="dcd"
                     /locus_tag="PLM90_07995"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMZ89353.1"
                     /translation="MILTDKEIQSAIAKGDILIEPYRADCLGTNSYDVHLGRYLAVYK
                     NHILDAKKHNEIDEIIIPDEGFVIHPGILYLGVTEEYTETHAHVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNTWTLEISCAQPVRIYAGMPIGQLIYFEVKGDITNYYNKKENAK
                     YVNRTIRPVESMMWKNKF"
     gene            complement(13275..>14021)
                     /locus_tag="PLM90_08000"
     CDS             complement(13275..>14021)
                     /locus_tag="PLM90_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89354.1"
                     /translation="VPGNIFALLLMSDKGLVMAGDLYGGVYVIDTATRQEVNRFSWDG
                     AAVYAFLRLDDDRVAIGSGTGNLIIRNINSHTTEQVINIGQEAIRSLVKHPTLPQIAL
                     GCSDNCVHILDADTLKTITVLHGHVNSVFSVQYHPNGKHLLSGARDAQIRIWDVADGY
                     SCLQTLPAHMFTVNDIKFHPDGHLFASAGRDKHVKIWDAETYALLKVIDREKYDGHIN
                     SVNKLFWSKWQNTLISCGDDRTVMGWGLIS"
BASE COUNT         3959 a         2698 c         3105 g         4259 t
ORIGIN      
        1 tcaagtaacc tatcaatggc aacaagcgca cggtggctca gttaatgacg gggcctattc
       61 catcaacgca acatcagatg gcggatatat cgttggtggt tatagctatt ccggaatttc
      121 cggcgacaaa acaatggcct catttggcgg tgaagattat tgggtactta aactggatgt
      181 cgatggaaat attcaatggc aaaaagcgta tggtggaagt gggcttgaca ggttgaacaa
      241 ggtagtgcaa attactgatg gaggatatct cgttggcgga tattcccaat caatgatttc
      301 cggtaataaa acagaaaatt cttatgccgg cacacaagat gtatgggtgc tgagattgga
      361 ttccttagga aacattctgt ggcaaacatg cctcggtggc agtgggattg atatgcttga
      421 ggacgtaaat gaaactgctg acggcggttg tattattggc gcctattcta caagcggaaa
      481 tagcggagac aaaaccgaag gttcgaaagg cgtatatgat tattggatta ttaaactgaa
      541 tagcaccggg ggcatcatgt ggcagaatac tatcggtggt acaggatctg attattgtcg
      601 aggcgcacaa caattgcctg atggcggata ttttatcagc ggatattcag gttcgccaat
      661 aagcttcgat aaaactgaag catgtgtggg accatttaat aacaatgatg cctggatcat
      721 gaaactcgat gcttcgggaa atatcatatg gcagaatgta ataggcggtt cctcaaatga
      781 taatctgacg gcagctaaat taacacctga taatggtgca atacttgctc tttattcttc
      841 atcgaatatt tcaggcgata aatctgaaaa taccatagca ggcgtaacca cagatgccga
      901 ttattggata gtaaaagttg atgcagccgg gaatgtaatg tgggaaaata caatcggtgg
      961 tatagatgtt gatctatgtt acgatgttac acttatgtca gatggcggat atgctttggc
     1021 aggatactcc ttatcgccaa tgtcaggaga taaaactaca ggtgtttatg catcatcctt
     1081 tgattattgg atagtaggtg tagacgcact gggtaatgtg ctctgggata gaaattacgg
     1141 cacctatctg gatgattatt gttacggtat taccacaggc gccgatggta tgttggtagc
     1201 agcaggggat gcagaatcag tgactacagg tgataaaatt gtgacaggaa acggatggaa
     1261 agacttttgg ttgattaaac tgcgcaatgt atgtattccg acagatgaag tatgcaatga
     1321 tatcgacgac gattgtgatg gtattattga tgaaggggtt gttgagaatg tatatatcgt
     1381 cgacccgggg atcacaactg tttgtcaagg tacacctgtg aatatggcat ctgtatttac
     1441 cgccggggtg ccgcaatggt atagaaatgg tgttgctata ccgggggcaa ttggtatcta
     1501 ttacacagct acagtttccg gtaattacac ttgtgttact tcgagcgatt gcggcacaga
     1561 tacctcaaat gtggtgcata taacggtcaa taaaaatcct aaagcaagta tttcggctgg
     1621 aggaccaacc acattttgta tgggtggaag tgttactctc actgaaatgc catccgccgg
     1681 ctgtacatat caatggatga agggagcatc gttggttgca ggtgctacat ccaatgtgta
     1741 tgtcgccacc actaccggaa attataaatg cctcgttaca aagactgcaa caggatgctt
     1801 caaaacatca aatactattc cggtaaatgt tatttgcaaa ttggatggca gtgataatat
     1861 ttcaatgtcg gtatttccaa atccaacgca tgatttaatc atgctcgaaa ttcctgaaaa
     1921 tgaaattgtt gatgtatgga tatatgatca atcaggtaaa ctgttaacct cactgaatgc
     1981 attcgaaagt ggcgatattt ctgtagaaaa tcttcctgca ggaacttatc ttgtaaaggc
     2041 cacgaatgat ttgcatcaat ggaatgccta ttttataaaa caataacatt ttaatcccga
     2101 gcaaaggtca aaaaaaaggc catcatgaat agtagttatg gcctttttat tttattcgat
     2161 tttttttacc tggctatgat cactttctgc acaataggtt cctcgttact attgttaatt
     2221 actaccatat aagtacccgg agctacatca ctcacatcca tagtgaattc cttcgaattg
     2281 gttgtgtagc tttttatcaa agatccttgt aggttatata actcaacaaa tgcatctata
     2341 gtttctgcag gagaaacagt ttcaattgtg attgaatttg atgcgggtat cggatacact
     2401 ataacatctt caacataagt gttttcatca cctatcctac aactggatgt gctgaaattc
     2461 acaacatcac tccattcaga atttaaagca ggtgtgttgt cataaactgc ctgaacctgc
     2521 cattcatatg ccgttccaca agttagtgac ttaatcagtt tgctcgttgt ggtggtcatt
     2581 ttggtaatcc aaacagtagc accaaccgga cgatatctga accgatattt tatagcaccc
     2641 ggcacactgc tccatgtcag attggctttt gttgtggcaa cagtaactaa acccggattc
     2701 ggagcttgag cagtaacagt tccggtcagg ctaccggcaa caatatttgc ttcgggcgac
     2761 atgcttattg cataatcctc gatattaagt gagccctcgt caacactcaa acgtatccat
     2821 ccggtatata aattaccgcc atcttttact ttgaagggaa taaccctgga atcggcatga
     2881 aaccaaaatc ctccggtaaa catgtaaaaa tcttcaggat tatcgcaatc cccaaaatag
     2941 ctccagcttg aagcgaaata acctttaaca cgctgaataa agaggttttg gtgactttgt
     3001 tcctcgttaa taggttcaat gaatgaaccg gcagtaggat tggcatgaat catttggcca
     3061 ttgctgtaat tggcaacatt ggcaaaattt gcagcattcc aaccaatact acaccatcca
     3121 atatactgtg atgatgctat ctgtacctga ttggaaccta gtggtatggc cgcggcttgt
     3181 atgaaactgc ctacagcatc ccaccctgta taagtggtaa aaccctccag tctgaaacga
     3241 aaatcatcca ctccatcatg atttaaatca atattagcaa tcgagtaaaa ccaaccaaaa
     3301 gggtcgccag caggaatatt gatatccgga tcaatgtcct gatacatggc tgtttgattg
     3361 gagacatcac cggaggccaa aatggctgcg gcagcaagac catactcttt tagtttcata
     3421 aggaaaattt tggggttaat aattttacta tgtgaaaata agaatatttc gttattttta
     3481 gaaaataaaa tattatcctt atgaaactta gctgcgctaa tcttgtggtt ggtctattca
     3541 ttttacttgt ttcatttctt acgccaactt tacatcttaa tgctacatct ccgtcattgc
     3601 cggacggtaa cgacttgcgt caattgacaa gtgcctcttt tacggatcct gccaataatt
     3661 tcatggtgaa tattgaaggg aatgcgttta atattaccca taaagagaat ggatggtcgc
     3721 ttgatgctcg gttaaccgga atgtccagag cgaatgctac ttaccctatt gaggttatgg
     3781 cgggaaatgt aaataatagt aaaatattga catttcatga tgctaaaatt gatattattt
     3841 atcagctcac ttccatggga atgcgtcagg attttgtgct gaaggagaaa ccggcaggcg
     3901 agggagatat ttctttacaa ctggcgtttt cttccgctca aattgaattt tcatgcaacg
     3961 ggtctacatt aaccggttca ttaaatgaaa ttcctgttta tgcatataca gccctcaaag
     4021 tttgggacgc aaataatgct ccgctggctg cttcaatgca atggcacgaa ggtgttctgt
     4081 ccctgatatt gaatgatgat aatgccgtat atcccattac tatcgacccg gtgagcatta
     4141 caccgaatgc tattttaacc ggaaacatgc tctcatcaca atatggaaat gatatcgata
     4201 atgccggcga tgtgaatggt gatggttatg atgatttaat tgtaggctca attttttatt
     4261 ccaatggtca gtcttcggaa ggagccgcat acatttatct tggttctgct tcgggtatat
     4321 caacaacacc acaaattatt cttgagtcga atcaaaacag tgccaatttt ggcaaggctg
     4381 ttgccggtgc gggcgatatc aataatgatg gttatgacga tattgtgata ggagcataca
     4441 attacgataa cggacaaaca aatgaaggac gtgcttatat ctatatgggc tcagcaagtg
     4501 gtatcagtgc cacgcctgcg cttacattag aagtaaatca ggcgcaatgt aattttggtt
     4561 cctctgttgc tgctgccggc gatgtaaata atgatggatt tgctgacgtc attatcggcg
     4621 catataatta tgacaacggg caaacaaatg aaggccgcgc gtatgtatac cacggctctg
     4681 ctacaggact aaatgcaaca cccgtttcta ttatggaaag caatagcgca caagcctatt
     4741 tcggttggtc tgttggcggt gatatggatg taaatggtga tggatttgac gatgtaatag
     4801 taggtgcata taaccaatct aatggtcagg caaatgaagg ccttgcttat gtatttcacg
     4861 gatctgcctc aggaattatt acaacacctg ccgctacacg tgaaatgaat caggtaaacg
     4921 catttttcgg cgaatctgtg agtaatgcag gtgatgtaaa taatgatgga tatgacgatg
     4981 tgattattgg cgcctattta tttgataatg gtatgtctac ggaaggtgcc gcatttattt
     5041 atcatggatc tccgacaggc atcagtgcag ctcccataac acaattagaa gcgaatatta
     5101 tgtttgcata ctttggatat tccgttgatg ctgcaggtga tattaatggt gatgggtttg
     5161 atgatgttat tgttggtgcc tatatttttg aatatgatca acaggatgaa ggtcgtgcat
     5221 ttgtttttcc gggttctgta acaggagtca gcgatgttcc cgatgcggtt atggagcgaa
     5281 atgaaataaa tgcaaacttt ggttatgctg tatgcggtgg cggcgacttt aataatgacg
     5341 gatttagtga tgttgctgtt agtgcgccta aaaaagattt tgctgttact gatgcaggag
     5401 gcgtttttgt ttatcacggc agtaattgta caccacattt attatttgaa gattttgacg
     5461 gcgatggtta tggagccttt aattatccta ttgtttcttg tgatgccacc gctgccggat
     5521 atgctgtgaa taatgacgat tgtgtcgatg acaatgcagc acaaaatcct acaacagtat
     5581 ggcatcttga tgctgatggt gatggatatt atattgatga tcctgcaggt atcacacaat
     5641 gcgaatctcc cggaacggga tatgcttttg ccggtattct tggtggcgat gactgtgatg
     5701 atgctaaccc tgccgcaaat ccgggagcaa cggaaatggt aaatggtgtg gatgataatt
     5761 gcgacggcat tatagagcct tgggcatggg agggtgctgt taccttagag ggaaatcaaa
     5821 catctgctta ttttggttcg gctgtttcct cagccggtga tgtgaataat gatggatatg
     5881 atgatatcat agtggctgca aatggttgga ctgatgtcca atcggatgaa ggcgccatat
     5941 ttatttatca tggctctgca acaggggtgt cgccaacacc aaatacttca attcaaagta
     6001 attatgcctt tgcctatttt gggtactctg tggcagctgc gggtgatgta aatggcgatg
     6061 gatatgatga tattattgtt ggggccacta attatgaatt aggtcaatca aatgaagggg
     6121 ctgcctttat ttaccatggc tctgcaacag gaattaatac tactcccgcc accattttgc
     6181 aagctgatca ggccgatgca ttttttggtt atgatgttgc cggtaatgcc gacttgaata
     6241 atgatggtta tgatgatgta atagtaagtg catatatgtt tgataatgga caaacaaatg
     6301 aaggtcgggt attcatttat tacggttctc cttcaggtgt gaattcaaca ccggtactga
     6361 cattggaatc tgatcaacca aatgcttatt tcggtagatc tatcgccata aatggtgatg
     6421 ttaataatga tggatacgaa gatctggtta ttggggcttc acattatgat aatattttgt
     6481 ccggagaggg tagggtgttt gtgtatctcg gaactgcagc cggtattgat ccactgcaag
     6541 tttcttatca tgaaagcaat caaactttgg catacctcgg taggtctgtt gctatgagtg
     6601 atgtaaatgg cgacggctat gatgatgtga tcgccggagc acattattat gacgatactg
     6661 aaattaatga aggcgctatt tttgttttca atggatctgc atcaggtgtt tcaaccacgg
     6721 ctgatgacat tatcgaaaca aaccagggtg acgcattttt aggtatcagt ctggcatcgc
     6781 tcgatgatat caataatgat ggttatgacg atattatcac cggtgctaca gggtttgatg
     6841 gtgcagaaga agatcaggga aaagcatttc aatttaatgg cagcgcatcc ggattatcac
     6901 cttcaccgga agcaggtgta tttgagcgcg cacaatataa agccgactat ggtgtcgctg
     6961 ttgcaaatgc aggcgatgtg aatggagatg gtataaatga tattttaatt ggtgccgata
     7021 aatatgacgc tccgtcttca gatgaaggaa ttgcaatgtt gttcctgtca tgctcaacac
     7081 aattttttgc agacgccgat ggagatggct atggcgatga tgctacgctt gtgaattcat
     7141 gtaatgctcc tcttggatat gtatctatcg gaggcgattg cgatgatgac aatgcatctg
     7201 ttcatccggg agcaactgag atatgcaatg gtattgacga taattgtaat ggaaatattg
     7261 atgaagggtt tgaattgtct attactattg ctgctttggg tgctactaca ttttgtgaac
     7321 cgggaacggt cactttatcg gcttcatata ccggtacaag tattcaatgg aaacgcaatg
     7381 gaacaaatat agcaggagca acgtccgcaa catatactgc aaatcaaagt ggcacttatc
     7441 gcgcagaaac atccaatgca tgtggctctg atttgtcgga aggaattgtt gtaactgtaa
     7501 acaaaaagcc tacagccaca attacagcgt caggcgcaac aacattctgc gcgggaggtt
     7561 ctgttacatt gaacgttacg ccggttgccg gttgtagcta tcagtggtat aatggcgcta
     7621 ccgcaattgg cggtgcaaca ggaacggcat atgtggcaac aactgcggga agttacaaat
     7681 gtcgtgtaac caaaacagca actggctgct ataaaaattc aaatatcatt gatgtcacag
     7741 ttccttgtaa ggaaagcctt aatagtgagt taggttttag tgtttttcca aatccggcaa
     7801 atgaacaatt aaatgttact gtttctgagg agatagatgg cgttgtgcac atcacagacc
     7861 tcgcaggtaa tattgtcatc gcaacagaaa tgcataccgg caatatggct atcgatattt
     7921 cgattttgcc ggcaggtatg tatatgttgt ttatactgca agaaagtaag cagcttgaaa
     7981 ctaaattcat caagcagtaa atattctact atgagaaaac tactctttac ctcagcattt
     8041 gtgtgcttgc atattgcttt tttgcatgca caagattgga gtttatttcc tgctgataat
     8101 cattcgtatt atcggaataa tgatatgatc tacacggtta cacctgattc caactatgtg
     8161 tcaggggata ctgtcttcac gcttttccaa tttagtgata cacctttatc agcaagtgga
     8221 tgcacaaatg aaatcgagga ttatctatat gccggaggct cgcttttcct gtttccgtat
     8281 gttgattatc ttgctaaagt tggagacttg actgtaatta cttttggtag cggtgaatat
     8341 cagatgcggt ttaatacaat gtgtgctaaa gatgatactt ttcatctagg cggtaacctc
     8401 gatccggatc tctatataat ttgtaatgat gtgcgaattg acaatatttt cggcacttta
     8461 gatagcgttc gtgaatttca ggtgttacat gatagtggtt acgatatatc tcttgaaaac
     8521 acctttattc taagcaagac ctttggattg ctttcatttt atgatttcaa ccaattacca
     8581 tattatgcta ttgcaagtat gcagtacttt gaaatgattg ggattgaagg acccatcgga
     8641 gataaaggat ttaagcctcc attctggaag gattattttc cactgcaggt ttcagatgtg
     8701 ctgatatggg ataggcatat ttatagccat attcctatgt actatattga ttatcacctg
     8761 tattataaag attctgttat tgcgagaatg gattacccgg atagtattgt gttttacttc
     8821 gatcgttggc agaaggatac ttccggtatc ataacacaat tgccaactta tcgtttaagg
     8881 cataagcggt cagaattaat caacatgttg gaaggtccaa ccggccgatt taaacatatc
     8941 gaagtgacac ccggatgcta ttattttcaa cataatattt tgaaatccga tggattaaaa
     9001 atgttgttct caggtgatga tacaatttac tcctatcagt attttggagg tgtctacgaa
     9061 atttatgacg gttgttttgt tggagaaaca tatgatgttg gcaaatcatt tactttacaa
     9121 acagatcatg gacttgttgc cggttcatat agtgtaggcg atggcaatga ttatattgtt
     9181 cttgcaggtg gcattattaa tggtgagtcg tttggaattg ttgattttga aacagtcgat
     9241 attgcaaatg acttaatccc tactttttct ttatttccta atccggcttc cgaagctgtt
     9301 aatattgtac tgcctgacaa tgtgagtggt gatatccgaa tatttaatac ggcaggcaat
     9361 tgcattgttc atgcggtgag taatattggg aagctaacat tggatacaag taattggcct
     9421 tcaggaattt acatggttca agttgtaaca aatgccgact catcatccca gcgattgatg
     9481 aaattttaga tataccgttt tgtattgata tatgttaaag atcatgagcg gttgtaacat
     9541 tcagattttt cgttcgttat atgtttttag ttcactccaa aactaactga catggaaaca
     9601 ctgcgtgttg ctttcgtttt aattgcctgc acaatttctt cagccgtcaa tgcacaatgg
     9661 agtgccgaag ctcaattggc aggttcaaac tttcttggtg cctcactgaa tctcaattac
     9721 agtttgtcga tctctgccaa tggtgatcat aagcttacac caaccattgg cctcgggctt
     9781 tcaagtaatg aatggatgcc gacatcgcgc atgatcatcc atgcaggatt gaattattcc
     9841 tacaagcgat atggcattgg tgttgaggct tcccaattcg ggccattatt tccaaaagat
     9901 ggcgttgcat atgataatga gctcatcgtt tttccaaaca tcaattattt aatcgttgat
     9961 cgcgatatgt ggtatctcaa ggcttcgctt ggggcatatt ttgccttcag cgaattggat
    10021 ggcatcgatg ataaacctac acctacactt tctttcgaag gcgatgtcat ccccggcgcc
    10081 ggtttaagtg tggggatcaa attatttaaa tgaccttgtg aaaaatgatc tgcaaaaaaa
    10141 aggtattgtc atcacaacaa tacctttttt gtattaagcc gttttcgact gtttcttttt
    10201 attcttccag aaaggttttt tcgatctggg tttttgacca cctgcagacg cggtttgctg
    10261 ctgcttcggt ttttcagtgc gaggtcgatt gcgctgtgca tgcaatggcg catccggagt
    10321 tgcaatctcc tttttatatt caggatgatc gatatgcacg agaattgtct tgccgatcaa
    10381 tttttgaata tcgcgcagat atgctctttc ttcctcatca cagaatgcaa tggcaacacc
    10441 actgcttccc gcgcgacctg ttctaccgat gcggtgaaca taagtctctg gaatattagg
    10501 caactcataa ttgatcacat gtgtcaactc ttccacatca attccgcgcg ctgcaatatc
    10561 ggtagctacc aatacctgcg ttttacccga tttgaaattg gttaatgcgc tctggcgact
    10621 attctgtgat ttatttccat gaatcgcttc tgcagtaaat cctgcacgca caagatcttt
    10681 gcaaactttg ttggcaccgt gctttgtgcg tgtgaatacc aggcaacttt ttatatcctt
    10741 cgttttcagg atatgatgca ataattgttt tttatttccc ttgctcacaa aatacaccga
    10801 ttgctcgatc aggtcaactg tcgatgaaac aggtgttacc gcaactttta cagggtgatg
    10861 cagcaatgag tcggcaagcg cagtaatctc tttcggcatc gttgctgaaa agaataatgt
    10921 ctgctttttc gcaggcaact tcgcgattac ttttttcaca tcatgaataa atcccatgtc
    10981 caacatgcga tctgcttcat caagcacaaa tgtttcaata tgctgcaaag aaataaatcc
    11041 ctgctgcatg agatccaaca agcgaccggg agtggcaacc agaatatcga tgccgcgttt
    11101 aagttgctga gtttgtgcat gctgtgaaac accaccgaag ataacggtgt gatacagtcc
    11161 cacatattta ccatacgcct caaagctctc accgatctgt atggcgagtt cacgggtagg
    11221 agtgaggatc agtgcacgaa ttgcgcgagg atgcttgttg ttggccgcat gcaatcgctg
    11281 taacaccgga atagcaaagg ctgcagtttt acctgtgcct gtttgcgcgc aaccaaggat
    11341 gtcttttccc tgaatagccg caggaatgga ttgttcctga attggggtag gattttcata
    11401 cccctcttgt tgtaacgcct tcaatatagg cgcaataagc cctaatttgt caaatgacat
    11461 aaatagttag aatgtataag gatggaaatc taacaacgag tgaaggacta agtcctacct
    11521 gtgttcggcg cagttataac gtctgcctta tggataaaaa tgaatgctgt tcacccgttt
    11581 aaaccttgtt ttcatctgaa cactcttccg ataaccaaaa cgggaacgcg aggcaaagat
    11641 agggaaaaaa ttgaagaaat ggtgatgtgc tgatgggctg atgaggtgat gggctgatga
    11701 ggtgatgaga tgatgtgctg atgaagtgat gagatgattt tgtggatttg gaggatgggt
    11761 tgatgaggtg atgcgagtga tgtggaagtt gttatgatga gggggaaatg taaaacttac
    11821 tgatcagatg attggttttt aatttatatt gtggtttgtt gatggttggt tcaagatagt
    11881 aggggatgca tacttatttt gattcaagaa gtaagaggga tttattcgaa atgttaaaat
    11941 cagtatttat tcaaatcaat gggagttaca atgggggcaa aacataagcg gttatctttt
    12001 attttaatta aaattgaatt agtataagcg gttacaaact ttgatattta ttgattgcca
    12061 aaatcaaaca ttatttattt tggtgcataa taatggcata tatcaatagg aaaaacgaag
    12121 tattagctat gtccttcgat ttttcaaagg cattggttta ttactgtaag caaattgaag
    12181 agacggggca caagcatatc gcttggcaat tactacgctc tggtacttca gtttgtgcga
    12241 atattttcga ggcgcaaaca cccgaaagca aatcggattt tattcacaaa ttaaaaattg
    12301 cagcaaaaga atgtcaggaa actatttatt ggttgctact tatcaacgaa acaatagagt
    12361 cggataagtc tgaagtctat cttaaaacag cccactcaat tgcacgcctc ctatccacaa
    12421 tcattgcctc cacaagaaaa tcactctaat cctccacaac ctcctcaccc ccccatcatt
    12481 acatcagccc atcccccctc atctgccaat tagccaatca gcccatcagc ccatcagcac
    12541 atcacctcat cagcccatca aaacttattc ttccacatca tactctccac cggacgaata
    12601 gtgcgattta cgtatttcgc attttctttt ttgttatagt agttggtgat atcacctttc
    12661 acctcgaagt aaatcaattg tccgattggc atacctgcgt aaatgcgaac aggttgtgcg
    12721 catgagatct cgagagtcca ggtgttgcag aaaccaacat ctcctttacc cgcagtagcg
    12781 tggatgtcga tacctaatcg gccaacactc gatttccctt ccaaaaatgg aacatgtgca
    12841 tgtgtttcag tatactcttc tgttactccg agatatagga tgcccggatg aataacaaag
    12901 ccttcatccg gaatgatgat ctcatcaatc tcattgtgtt ttttcgcgtc gaggatgtga
    12961 tttttgtaaa cggcaaggta cctgcccaaa tgcacatcat acgaattcgt gcccaagcaa
    13021 tcagccctat atggctcaat caaaatatcg cccttagcaa ttgcactctg gatctctttg
    13081 tcagtaagta tcattgttgt ttcattattt aattcttctt tcgtgactcg tgactcgtga
    13141 atcgtgaatc gtgattcgtg agtggtgact cgtgagcctg tgggttgact atactccaaa
    13201 ttaacttccg cacccctgca tttcaattgg ctaatcagct aattggctaa ttggctaatt
    13261 ggctaattgt ctaatcagct aattaacccc catcccatca ccgttctatc atcgccgcac
    13321 gaaattaggg tgttctgcca cttgctccaa aacaacttat tcacggagtt gatatgtcca
    13381 tcatattttt ctctgtcgat cactttcagc aaagcatacg tttccgcatc ccagatcttc
    13441 acatgtttat cgcgacctgc ggaagcaaac aaatgaccat caggatgaaa tttgatatca
    13501 ttcacggtaa acatatgtgc cggaagggtt tgcaggcaac tatagccatc cgccacatcc
    13561 catattctga tctgtgcatc gcgagcgccc gatagcaggt gttttccgtt tgggtgatat
    13621 tgcactgaga agacagagtt tacatgtcca tgcagtacgg taattgtctt caatgtatct
    13681 gcatccaaaa tgtgtacaca attatcgctg catcccaagg cgatttgtgg aagggtaggg
    13741 tgtttaacca gcgaacgaat ggcctcctga ccaatattaa tcacttgttc cgttgtatgc
    13801 gaattgatat ttcgaatgat gagattaccc gtacctgagc caatcgccac gcggtcatca
    13861 tcaagtcgca gaaatgcata aaccgcagcg ccatcccaag aaaatcggtt aacttcttgc
    13921 cgggttgcag tgtctataac atacacacca ccatagaggt cgccggccat caccagccct
    13981 ttatcagaca tgagcaatag ggcaaagata ttccccggaa c
//
LOCUS       DAOQOU010000076        14009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2244_length_14009_cov_6.363910, whole genome shotgun sequence.
ACCESSION   DAOQOU010000076 DAOQOU010000000
VERSION     DAOQOU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14009
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2244_length_14009_cov_6.363910"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLM90_08005"
     CDS             complement(<1..1096)
                     /locus_tag="PLM90_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ89355.1"
                     /translation="MSAVERVIHSPLSTFYKWEATQPDAMYLRQPVNGTWHTYSWREC
                     GEQVRRMAAYLRSLNYAPGSKIAILSKNCVHWMLSDYAIWMAGYVSVPMYPNMAPTTV
                     RQILDHSESKLLFVGKLDDWEYLKPGVPETLPCIAYPFYSVPGFPTWDDIVSKTDPIT
                     DNPDFPMDNLCTIMYTSGTTGVPKGVMHKFYNFAYAAEMAFPALNIDNTAIFFSYLPM
                     CHIAERLLIEMGSLYTGGQVSFAESLDTFPKNLQDTAPTIFLAVPRIWAKFQEKILEK
                     MPQKKLDRMLKIPIIKGVVKTKIKKALGLHNADQVFSGAAPISVDLINWFWKLDIRIQ
                     QAYAMTEDCCYSHTNYKDRNRIGTVGLRFPG"
     gene            1172..2218
                     /gene="thiL"
                     /locus_tag="PLM90_08010"
     CDS             1172..2218
                     /gene="thiL"
                     /locus_tag="PLM90_08010"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMZ89356.1"
                     /translation="MNEQSGRTEISSLGEFKLIDLLTQNTEIKHAATLKGIGDDAAVV
                     DHSHLVSVISTDMLVEGVHFDMMYTPLQHLGFKAIAVNVSDICAMNATATQVLVSIAI
                     SNRFSVEALEALYEGIHHACQYFDVDLIGGDTTTSLKGLVISVTVIGEANESDIAYRN
                     GAKPGDLICVTGDLGAAYCGLQLLEREKQIYLENPEIQPDLENKKHIVGRFLRPEARK
                     DIITYFKEHGIKPTSMIDISDGLSSEILHICKQSACGAIIYEDAIPIHEETYQQATKF
                     NIDPLVCALNGGEDYELLFTVDESMGEKIAQHAGISVIGKITDKASGTFLKTRNENLF
                     KIEAQGWNSFPDQQ"
     gene            complement(2227..3570)
                     /locus_tag="PLM90_08015"
     CDS             complement(2227..3570)
                     /locus_tag="PLM90_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89357.1"
                     /translation="MHIRALQPVNQYITLQRISAIALILCCFSATAQINCENDTSFHI
                     PIVDLQTDYFGVHQGGLYPDGLNTMPAAHADSGIAIAESLLPINYDGQEDTSYGKFVF
                     LGLGNEAAGKIFNKFITQHDDLGLGDSCMRIVNACMEDYTLHDMYGADASDAYWNDVN
                     DYLQSQNLKKKQVRAVWLMTTNYDDTLITEDAYIDSVKNTYIEVIRKMKKQLPNLSLL
                     YISGLPYGGYADTMFDGHEFFEEPIPYLNDFAIKAVIDAQINGDTSLIYSGEDADAPW
                     IAWGPNVWADGRNLRAYDNLRWLCPGDYASGDNGIMLSGTGQTKVADRLYTFLTSEPT
                     TLPWIYGLPYDCFTEPDFVEPEDSVIVPEDEILWITQNPVKGVIKFVVNVETNDKAQI
                     YVFDMIGNQIVEGVFNKIEPGRVLSVNVTQNARGLYILSVFVENKVYNKVFYLDN"
     gene            complement(3575..4906)
                     /locus_tag="PLM90_08020"
     CDS             complement(3575..4906)
                     /locus_tag="PLM90_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89358.1"
                     /translation="MRKLILACTLLAATTIAQPVFGQLNCANDSTGLIPLVDLGTDYY
                     AGIYQGGLYPGGSNIMPSGHMINGLKIMKKLKPLDTAGVVNYATGKIVLGGFGASTVG
                     GPFNHMIQLMKDYNDLNPCMKAVNTANGSDGIEAMYIGNEEYFDYIKIYKLAEKDLTP
                     IQVQVGWLMHSSRIDSNGTDPIGYVDSLSKRFLIALNTMLYYYPNLKVVYLSGFPYGG
                     YADPMKALYHVIKEPSSYHHNFAMKSLIERQITGDPALKYKGAGIQVPYLAWGPNIWA
                     DGKNPRELDSLTWNCETEFAIDGGGYHLTNEGKDKLANILLQFFRTDTLSEGWFMDGP
                     KWASCGAGRLADGSIILPEEPTILTKDEITVFPNPSSGQYYVDFDEVLTEAIQVKVVN
                     QVGEVVLEESYGGIQPFSFYQFNLSDQPAGIYYFEVVANGEHFSQPIILNH"
     gene            complement(4998..6308)
                     /locus_tag="PLM90_08025"
     CDS             complement(4998..6308)
                     /locus_tag="PLM90_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89359.1"
                     /translation="MQALRLLRNGSLLLLMTGVQLVYGQLQCDNDTSYLRSLIDLKTG
                     LYLGYQGGLYPDGSNTMPYSHYADGMNISRQVFPLNADGAVDLVDGKVGFICLGASTA
                     GNAFNHFKTVCEEDPAVNPCLKFANCAVGAKGLEVMIDTIEDAWYWDDEVMPDLDYAG
                     ITRHQVEIIWVMVTSRIDTILIWPYQPQEVANKYEILMQVLLKKFPNLKQVYLSGFNY
                     GGYADPTKEFYDMIMEPSSYWNNWSVKFLIERQIEDDPGLDYKAPDRVAPWLGWGPHI
                     WADGLRANTTDHLRWVCEVDYKPDGGGYHMSNTGKDKMGKLIYDYFINSPIAADWFKY
                     GARWTSCDPDFKVEEPSTEATLTFSPNPAGNDALLELDRWADLPVEVTIYNAMGQPLS
                     NLNFCPSNSYIAYPVSLAGLPTGTYYIVARQTGLRVMSLSFLKQ"
     gene            6381..7013
                     /locus_tag="PLM90_08030"
     CDS             6381..7013
                     /locus_tag="PLM90_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89360.1"
                     /translation="MTDDNQQHALEDLKEIRAMMERSSRFISLSGLSGISAGIIAIIG
                     AFIGGWYINKNIDYVEGNIARENVRTEDVVFLLLLAGVILFLAIGAAIYFAIRKARLN
                     NLPIWNKAAELTLVNLLLPLGTGAIFCLILFTYGLYILIAPATLVFYGLALLNASKFT
                     LSEIRYLGVSEIVLGLIAMVIPGYSILFWALGFGLLHIVYGAVMYFRYER"
     gene            7010..7300
                     /locus_tag="PLM90_08035"
     CDS             7010..7300
                     /locus_tag="PLM90_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMZ89361.1"
                     /translation="MIEGLNKEFDNRVRLGIMSILMVNSDVDFNSVKNLLQLTDGNLA
                     SHMSALEKAGYVEVNKAFVGKKPRTTYAVSESGKKAFQEHLNALEKLIKMGK"
     gene            7445..8482
                     /locus_tag="PLM90_08040"
     CDS             7445..8482
                     /locus_tag="PLM90_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ89362.1"
                     /translation="MTSGKKPMIAHVWQLFIRWVELYTWRFILSLLLITFSIIVVWEP
                     FWFVSALDALRIPYAVFLVAFVMFFFLRNNYALASAGGIALLIILPGIWPYFKTANEI
                     PAEAKKEKLEIVESDLTVLHFNVKENNKKIETVAEAAVQSGAIIVSMQELKPISFPKV
                     DTIMRSIYPYVYSELDYPGFGMALYAKVPFTSTETLEFNGFPVLHCTVEIDGTEIHLL
                     AATTSTPTNDKDAQLQAKQFVFMSEQVLAMQGPVFLMGDMNSVPWSEGIEGLLKKTQL
                     KDSRKDLSATYPAQSPLQIPIDYIFHSKEFTCNKFSTVGGTTSNHLGLMGYYTLRSTK
                     KSKIQDDKKAQ"
     gene            8463..8816
                     /locus_tag="PLM90_08045"
     CDS             8463..8816
                     /locus_tag="PLM90_08045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HMZ89363.1"
                     /translation="MTKKRNEIKSFADAFRGIGILFRRERHAQIHLVIAIAVVFAGFY
                     FSITDIQWLVIALTIAMVFTAEGLNTAIEKLSDAVHPERSEKIRDVKDIAAGAVLISC
                     IIAVIIGCCIFIPYL"
     gene            8819..9457
                     /locus_tag="PLM90_08050"
     CDS             8819..9457
                     /locus_tag="PLM90_08050"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HMZ89364.1"
                     /translation="MLKTLRLSRYHYLLCCTAFSMLLLLARMYHTNTLEYRFMAINLF
                     LAWIPYGVAKLMQNIPIGKWRPLIIVLAFVWLIFFPNAPYMITDIFHLSEFDAMPKWY
                     DLIMLLSFSWTGLLLGFFSLRKVLHRINAGNNFPQMIVSFIIFFLCGAGIYLGRYERW
                     NSWEIITQPRILISDFLGLLAQDAVMFQMATLSFVFATFFTMIFYFVPEETT"
     gene            complement(9458..10603)
                     /locus_tag="PLM90_08055"
     CDS             complement(9458..10603)
                     /locus_tag="PLM90_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HMZ89365.1"
                     /translation="MDLSYILNILGEERDQYFDAVTPPIVQTSNFAFKTVADFRDAIN
                     TEQQHHVYTRGNNPTVEIVRKKIAALEGTDDCLVFSSGVAAIAAILLHTLRSGDHVIC
                     VKSPYSWTQYLLTEVFPEYNITTTFIDGREMSQFEQAIQSNTKLIYLESPNTFWFELQ
                     DLEAVSKLARKHHIFTAIDNSYAAGIYQHPHKLGVDFVIHTATKYLAGHSDVVAGAVC
                     GNSEHIAGMFRRVFMGFGAVPSPHDAWLLLRGMRTLPIRLEKSASNAEMIVEHFMGHP
                     KIERIIHPFHPSHPQFALAQKQMTRGCGLFAMILKTENKTQVEAFCNSLERFLMAVSW
                     GGYESLIVPACIKIGDDYPAGFVRFYAGLEEANVLIADIEKALQYID"
     gene            complement(11027..11221)
                     /locus_tag="PLM90_08060"
     CDS             complement(11027..11221)
                     /locus_tag="PLM90_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89366.1"
                     /translation="MNFNPNLKIDEIIVRYALLLVVGIVAGVLHAYWMIPVVMGIFLT
                     AVLGWCPVKAMMKGKKVTAS"
     gene            complement(11328..12317)
                     /gene="meaB"
                     /locus_tag="PLM90_08065"
     CDS             complement(11328..12317)
                     /gene="meaB"
                     /locus_tag="PLM90_08065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMZ89367.1"
                     /translation="MTQLDLTTEQWLTGIRNGDRVLLSRAITLIESAVPAHREQALAL
                     LSACYPLSGSAMRIGITGSPGAGKSTTIESLGLQMLADNKKLAVLAVDPSSRISKGSI
                     LGDKTRMQQLSREASVFIRPTASGEHVGGVAAATRETIILLEAAGFDTILIETVGVGQ
                     GETAIYDMTDLFILLLIPGAGDELQGIKRGIMELADIILINKCDGDNVERAKLAAAEV
                     RRAIHLFQSKTSEWPVCVELISGLEGRGIPELRHQIDKYFELTKHNGYLESNRIRQRE
                     SWFQDQLHYAILRSVSEHPQYHEWLKSAEADIQTGQLLPSLAVHTLLKKLFQA"
     gene            complement(12386..13096)
                     /locus_tag="PLM90_08070"
     CDS             complement(12386..13096)
                     /locus_tag="PLM90_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ89368.1"
                     /translation="MQKLILAAVMVFTIATAFAQVDSAKTTTTVLEKEDPSRDRFLIN
                     VHWDGWMGAPDSMKIKGLSSGIGLHYMYDVPLKDDNISFAIGAGVSWSNYYNNSYFTY
                     DTSSYTVPVKFADTVQYSKNKFVLNYVEVPLEFRFRTDDKNGNRFKAAIGFKAGYAFS
                     NHTKYVGDDYISGSGDEIKFKQYRVKNVSTLQYGPTLRLGYSKINIEAYYGLAPIFTD
                     GEGPTGNPITVGVSFNPF"
     gene            13310..>14009
                     /locus_tag="PLM90_08075"
     CDS             13310..>14009
                     /locus_tag="PLM90_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89369.1"
                     /translation="MKQTVLLLTFACTLGTATAQWSEIFETGNIFGLIDISAPNDNIA
                     WACLSSDSAYRTADGGLTWDRIRLPEFEPNYQISNIYALDENTAFLCGQVIFTGEGPG
                     IVYKTSNGGVSWENVFQVDSNCTFTMHFKNELEGIMTCFTSVWFGGSTSFMKRTTDGG
                     NTWTSDITEPDAEFYGFTNQGMYAKGDSIWLCDIDGNMHYSDDMASSWTQIFTATFSN
                     VLRMPAFKNSDYGMV"
BASE COUNT         4019 a         2937 c         2911 g         4142 t
ORIGIN      
        1 caccggggaa acgcaatcca actgtgccaa ttctattcct gtctttataa tttgtgtgac
       61 tgtagcaaca atcttccgtc atggcataag cctgctgaat gcgaatgtcg agtttccaaa
      121 accaatttat caagtcaaca gaaattggtg ctgctcctga aaatacctga tcggcattgt
      181 gcaaccccaa agcttttttt attttcgttt ttacgacacc cttgatgata ggaattttca
      241 acattctatc aagttttttc tgtggcatct tttccaaaat cttctcttga aatttcgccc
      301 aaatacgtgg cactgcgaga aaaattgttg gtgcagtatc ctgcagattt ttcggaaacg
      361 tatctaaact ctctgcaaat gaaacttgtc cgcccgtata taaacttccc atttcaatca
      421 acaatcgttc ggcgatatga cacatgggga ggtaagaaaa aaatatggct gtattatcga
      481 tattcaatgc aggaaatgcc atttctgcgg catatgcgaa attgtaaaat ttatgcatca
      541 cacctttagg cacacctgta gtgccgctgg tgtacatgat ggtacataaa ttatccatcg
      601 ggaaatcggg attatcagta ataggatccg tttttgaaac gatatcatcc catgtaggaa
      661 aaccgggaac tgaataaaat ggataagcta tacagggtaa cgtttccggc acacccggct
      721 taagatattc ccaatcatcc agcttgccta caaacaatag cttgctttcg ctatgatcga
      781 gaatctgcct tacggtagtt ggagccatat tggggtacat gggtacggat acataacctg
      841 ccatccaaat tgcataatcg cttaacatcc agtgaacgca gtttttcgat aggatggcaa
      901 ttttacttcc cggggcatag ttcaatgaac ggaggtaggc agccattctg cgcacctgtt
      961 cgccacattc acgccaggaa taggtatgcc atgtgccgtt gacaggttgt cgcagataca
     1021 tggcatcagg ttgggtagct tcccatttat agaatgtaga cagaggagaa tggatcacac
     1081 gttcaacagc agacatatta tttagtattg tattggtcac taagataata atgacgacca
     1141 taaatgttaa atctttcagc aactttgcac tatgaacgag caatcaggac gtacggagat
     1201 atcttctttg ggggaattca agctaataga cctcctaaca caaaacaccg agatcaagca
     1261 tgcagccact ttgaagggaa ttggcgatga tgccgcggtt gtagatcaca gtcatttggt
     1321 gtcagttatc agcaccgaca tgctggtgga aggtgtgcat tttgatatga tgtacacgcc
     1381 cctccaacat cttggcttta aggctatagc agtaaatgtg agtgatatct gtgctatgaa
     1441 tgcaacagct acacaagtac tggtatcgat agccatctcc aatcgctttt ctgtggaagc
     1501 actggaggca ttatatgaag gcattcacca tgcttgccag tattttgatg ttgatttgat
     1561 tggcggtgat acaactacca gtttaaaagg acttgtcata tcggtaacag taattggcga
     1621 agcgaatgaa agcgatatcg cctatcgcaa tggcgccaaa ccgggagatc tcatttgtgt
     1681 caccggcgat cttggtgctg cctattgtgg tttgcaatta cttgaacgag aaaaacagat
     1741 ttatttagaa aatccggaaa ttcaacccga tctggaaaat aaaaaacata ttgttggaag
     1801 gtttcttcgt ccggaagcga gaaaagacat tataacttac tttaaagaac atggaataaa
     1861 acccacatcc atgattgata tttcggatgg tttatcatca gagatattac atatctgcaa
     1921 gcagagtgct tgtggcgcga taatttatga ggatgccatt cccattcacg aagaaaccta
     1981 tcagcaagct acaaaattca acatcgaccc attggtatgc gcattaaatg gcggtgaaga
     2041 ttatgagcta ttatttaccg ttgatgaatc gatgggtgaa aagatcgcac aacatgcagg
     2101 aatatctgtg attggaaaaa ttacagataa agcaagcggt acatttctta aaacaaggaa
     2161 tgagaatttg ttcaaaatag aagcacaggg atggaattca tttcctgatc aacagtaaat
     2221 gttgaatcaa ttatcaagat aaaacacctt gttgtagact ttgttttcga caaataccga
     2281 gaggatgtac aatcctctag cattttgagt cacatttacg ctcaacactc tacccggttc
     2341 aattttattg aagacgccct ctactatttg gttgcctatc atatcgaaaa catagatctg
     2401 tgctttatca tttgtttcaa cattgaccac aaatttgatc acacctttca caggattttg
     2461 tgtaatccaa agtatttcgt cctcaggaac aataacagag tcttcgggtt caacaaaatc
     2521 cggttcggtg aaacaatcat aaggcaaacc ataaatccaa ggcagtgttg taggctcgct
     2581 tgttaaaaat gtatacaatc gatcggcaac ttttgtttga cctgtaccgc ttagcatgat
     2641 gccattatca ccgctggcgt aatcaccggg acaaagccat cgcagattat catatgcacg
     2701 aagatttctt ccatcagccc aaacatttgg tccccatgct atccacggtg catcggcatc
     2761 ctcgccggaa taaattaatg aggtatcacc gtttatttgt gcatcaataa ctgctttaat
     2821 tgcaaaatca ttgaggtatg gaatgggctc ttcaaaaaat tcgtgaccgt caaacatggt
     2881 atcagcatat cctccatatg gcaatccgga tatatacagc aaagacaaat taggtaattg
     2941 ctttttcatt ttacgtatca cttcgatata cgtatttttc actgaatcaa tataggcatc
     3001 ttcagtaatc agtgtgtcgt cgtaattcgt ggtcatcagc catactgcgc gaacttgttt
     3061 tttcttgagg ttctggcttt gtaaataatc attcacatca ttccaataag catcagatgc
     3121 atcggcgccg tacatgtcgt gaagggtgta atcttccatg caagcgttta cgatgcgcat
     3181 acatgaatca cctaaaccca aatcatcgtg ttgtgtaata aatttgttga aaatctttcc
     3241 tgcggcctca ttaccgagcc cgagaaaaac aaatttaccg tatgaagtat cttcctgccc
     3301 atcataatta ataggcaata aactttcggc tatggcaatt cctgaatcgg cgtgtgcggc
     3361 gggcattgta tttaacccat ccggatataa tccaccttga tgtacaccaa aataatctgt
     3421 ttgaagatct acaatgggta tgtgaaatga agtatcattt tcacaattta tctgagctgt
     3481 ggcagaaaaa caacaaagga taagcgcgat tgcacttatc ctttgtaatg taatgtattg
     3541 attaacaggc tgtaatgccc gaatatgcat atgcttaatg attcaaaata attggctgtg
     3601 aaaaatgttc cccattggcc acaacttcaa aataataaat acctgcaggc tgatcgctta
     3661 aattaaattg atagaaagag aatggttgaa ttccaccata agattcttcc agcactactt
     3721 caccaacctg atttacaacc tttacttgta ttgcctctgt cagcacttca tcaaaatcaa
     3781 cataatattg acctgatgaa gggttaggga ataccgtgat ttcatctttg gttaaaattg
     3841 taggttcttc aggcaaaata atgctaccat ctgctaaacg acctgcgccg catgacgccc
     3901 atttcggacc atccataaac caaccttccg acaaggtgtc tgtgcggaag aattgcaata
     3961 aaatattcgc gagtttatct tttccttcgt tggtgaggtg atatccacca ccatcgatgg
     4021 caaattcagt ttcacaattc caggtcaagc tatccagttc gcgaggattt tttccatcag
     4081 cccaaatatt tggtccccat gcaagataag gcacttgaat tccggcgcct ttatatttta
     4141 atgcaggatc gccggtgatc tgacgttcga tcagcgactt cattgcaaag ttgtgatgat
     4201 agcttgaagg ttctttaatt acatgataaa gtgctttcat tggatctgca tatcctccgt
     4261 atgggaaacc actgaggtaa accaccttca ggtttggata gtaatacaac attgtattaa
     4321 gtgcgatcag gaagcgtttg ctcaatgaat ctacataacc gatgggatca gtaccattag
     4381 agtcgatacg agagctgtgc atcaaccaac caacctgaac ttgaatagga gtaagatctt
     4441 tttcagcaag cttataaatt ttgatgtaat cgaaatattc ttcgttgccg atatacattg
     4501 cctctatacc atcagaaccg tttgcagtat ttactgcttt catacatggg ttgaggtcat
     4561 tgtaatcttt catcaactga atcatgtggt tgaatggtcc acctacggtt gaagcaccaa
     4621 atccacccaa tacaatttta cctgtagcgt agtttacaac accggcagta tcgagtggtt
     4681 ttaatttttt catgattttc aaaccgttga tcatgtgacc gctaggcatg atattcgaac
     4741 caccgggata gagacctccc tggtaaattc cggcatagta atctgtgcca aggtcaacaa
     4801 ggggaataag acctgttgaa tcattagcac aattcaattg tccaaaaacc ggctgtgcaa
     4861 ttgtagttgc tgccagtagt gtgcatgcga gtattaattt tctcatggat taataaagtt
     4921 ggtatcgttc tgtaaaaaaa gttgtcaaag atacagaaag ccaaatgggc ttcctagttg
     4981 cgtggcgtca aaaataatta ttgcttgaga aagctgagcg acatcacacg taatccggtt
     5041 tggcgtgcta ctatgtagta tgttccggta ggcaggcctg ccaaggaaac aggataagcg
     5101 atataactat ttgatggaca aaagtttagg tttgaaagcg gctgccccat tgcgttgtaa
     5161 atagtaactt caaccggaag atcggcccag cgatcgagtt ctaataaagc gtcgtttccg
     5221 gccgggtttg ggctaaaagt taaagttgcc tcagtagaag gttcttcaac cttaaagtcg
     5281 ggatcacaac tagtccagcg agcaccatat ttaaaccaat cggctgctat gggtgaattg
     5341 atgaaataat catatatgag ttttcccatc ttatccttac cggtattcga catatggtag
     5401 ccgccgccat caggcttgta atctacctcg caaacccaac gcaaatggtc tgtggtattg
     5461 gctcttagcc catcagccca gatatgaggg ccccagccca gccagggtgc taccctgtca
     5521 ggagccttat aatcaagacc gggatcgtct tcgatttgtc gctcgatgag aaactttacc
     5581 gaccaattat tccaataact ggacggttcc atgatcatat cataaaactc tttggttgga
     5641 tcggcataac cgccataatt gaagccactg agataaacct gtttgagatt cgggaatttt
     5701 ttcagcaaca cctgcatcag tatctcatat ttattggcta cttcctgtgg ttgataaggc
     5761 catatcagaa tagtatcgat tctggatgtc accattaccc aaattatttc aacctgatgg
     5821 cgcgtgattc cggcgtaatc taggtccggc atcacttcat catcccaata ccatgcgtct
     5881 tcaatagtat ctatcattac ctcaagccct ttggcgccta ctgcacaatt agcgaatttg
     5941 agacacggat ttacagcggg gtcttcttcg caaactgttt taaaatgatt aaatgcattt
     6001 ccggcagttg atgctcccag gcaaataaaa cctactttac catctacaag atcaacagca
     6061 ccatcggcat tgagcggaaa aacctggcga ctgatattca ttccatcagc atagtgcgaa
     6121 tagggcattg tattcgagcc atcgggatac agaccgcctt gatagccaag ataaagaccc
     6181 gttttgaggt caatcagcga gcggagatac gaggtgtcat tatcgcattg caattgccca
     6241 taaaccagtt gaacaccggt cataagcaac aacaggctgc cattccggag gagtcgcaat
     6301 gcttgcataa ggtttcaaaa atagtgaatt atgatttcct gccgggcatt gggtaacttt
     6361 gctggtcaaa gcgctttact atgaccgatg ataatcagca acatgcactt gaagacctga
     6421 aggagatcag ggcaatgatg gaacgttcgt cgcgtttcat ttcactttcc ggtttatcag
     6481 gaattagtgc cggaatcatt gccataattg gagcctttat cggaggttgg tatatcaaca
     6541 aaaatatcga ttatgtagag ggaaatatag ccagagaaaa tgtgcgcaca gaagatgtag
     6601 tttttctgtt gctactggcc ggtgtaattt tatttcttgc aattggagca gcaatttatt
     6661 ttgccatcag aaaagcaaga ctcaacaatt taccaatttg gaataaagca gctgaactta
     6721 cattggtgaa tttattatta ccgcttggta ccggtgctat attttgtctg atattattta
     6781 cttacggatt atatatttta atagctcctg caaccttagt attttatgga ttagcattat
     6841 taaatgccag caaattcaca ctctcagaga ttaggtactt aggcgtttca gagattgttc
     6901 tcggtctcat tgctatggtt attcccggat actcgatatt attttgggct ttaggatttg
     6961 gacttttgca tattgtttat ggtgcagtga tgtacttccg atacgaaaga tgattgaagg
     7021 attaaataag gaatttgata atcgtgtacg attgggcatt atgagtattc tcatggtgaa
     7081 cagcgacgta gattttaatt ccgttaaaaa ccttcttcag ctcaccgacg ggaatcttgc
     7141 cagtcatatg tcggcactcg aaaaagcggg ctatgtagaa gttaacaaag catttgtagg
     7201 caagaaacca cgtaccactt atgctgtttc cgaaagcggc aaaaaggcct ttcaggagca
     7261 cctcaatgcc ctggaaaagt tgatcaaaat gggtaaataa agacccgcct cgaatgttga
     7321 acccttaagg attgcaaggc gtataggtca aattcggcgc actttttgca gcattatttc
     7381 atgtgataac gctttcccgt gcaatttcta ctttgaattt caaagtaagt tgtatatttg
     7441 caccatgacc tccggaaaga agccgatgat agcccatgtg tggcagctgt tcatacgttg
     7501 ggtggagcta tatacgtggc gatttatttt atcgctcctg ctaatcactt tcagcattat
     7561 tgtggtatgg gaaccctttt ggtttgtatc ggcattggat gccttgcgca taccgtatgc
     7621 tgtttttctg gtagcctttg taatgttctt ttttctgcgg aataattatg ctttggcatc
     7681 agcgggaggc atcgcattat tgatcatact tccgggaatc tggccatatt ttaaaactgc
     7741 gaatgagata ccggcagagg caaaaaaaga aaagctggaa attgtagaat cagatcttac
     7801 ggtgttgcat tttaatgtca aggaaaacaa taaaaaaata gaaaccgttg cagaggcagc
     7861 tgttcaatcc ggagctataa tcgtgtcaat gcaagaatta aaacctattt cttttcctaa
     7921 ggtggatacc attatgcgca gcatttatcc ctatgtttat agcgaactgg attatcccgg
     7981 ttttggtatg gcattgtacg caaaggttcc ttttacatca acagaaacgc tagaatttaa
     8041 tggatttcct gtgctacatt gcacggtaga aattgacggc actgaaatac atttgctggc
     8101 ggcaaccacc agtacaccaa caaacgacaa agatgcacaa ttgcaagcca aacaatttgt
     8161 atttatgtcg gaacaagtgc tcgccatgca aggaccggta tttttaatgg gcgatatgaa
     8221 ttccgttcca tggagtgaag gtattgaagg cttgttgaaa aaaacgcaat taaaagacag
     8281 tcgcaaagac ctctctgcca cctaccctgc ccagtcgcca ctacaaattc ccatcgatta
     8341 tatttttcat tcgaaggaat ttacatgcaa taaattcagt accgttggcg gcactacaag
     8401 caatcacctc ggactaatgg gttattatac cttgcgctca acaaaaaaat ctaaaataca
     8461 ggatgacaaa aaagcgcaat gaaattaaaa gttttgctga tgcttttcgg ggaattggaa
     8521 ttttattccg gcgagagcgg catgcacaaa tacatttggt aattgcaatt gccgtggtat
     8581 tcgcaggttt ttatttttcc attaccgata tacaatggct tgtaattgcc ttgactattg
     8641 caatggtttt tactgcagaa ggtctcaaca cagcaattga gaaattgtct gatgctgtcc
     8701 atcccgaaag aagtgaaaaa atacgtgatg taaaagacat tgccgccggg gcggtactga
     8761 tttcttgcat tatagctgta atcattggtt gctgcatatt catcccatat ttataattat
     8821 gctcaaaaca ttaagattaa gtcgctacca ttatttgttg tgctgcacgg cattttcgat
     8881 gttgctgttg ttggccagga tgtatcacac caacaccttg gagtatagat ttatggcgat
     8941 taatcttttt ctggcatgga tcccgtatgg cgtagcaaaa cttatgcaaa acattcctat
     9001 tggaaaatgg cgtccgctaa taattgtgct tgcattcgta tggttgatat ttttcccgaa
     9061 tgcaccatat atgatcacgg atatatttca cctttccgaa ttcgatgcca tgccgaaatg
     9121 gtatgacctg atcatgctgt tatctttttc atggacagga ttgttactcg gatttttctc
     9181 actgcgcaaa gtgttgcatc gcatcaatgc cgggaataat tttccgcaaa tgatagtatc
     9241 ctttatcata tttttcctgt gtggcgcagg aatttattta ggtcgctacg aacgttggaa
     9301 cagctgggaa atcattactc aaccccgaat actaatttcc gatttccttg gattacttgc
     9361 acaagacgcc gtgatgtttc aaatggcaac attgagtttt gtctttgcca ctttttttac
     9421 gatgatattt tattttgtcc ccgaggaaac tacttgatca atcgatgtat tgcaatgctt
     9481 tctcgatatc agcaatcaac acatttgctt cttcaagtcc cgcatagaac cgcacaaatc
     9541 cggcaggata atcatcgccg attttaatac acgcgggcac aatcaatgat tcatatccac
     9601 cccaactgac agccatgagg aagcgttcga ggctattaca aaatgcttca acttgtgttt
     9661 tattttctgt tttaagaatc atggcaaaca atccgcaacc acgtgtcatt tgtttttgtg
     9721 caagtgcaaa ttgcggatgg ctaggatgga aaggatgaat gatgcgttca attttcggat
     9781 gtcccataaa atgctcgact atcatttcag cattagatgc acttttttcc aatcgaattg
     9841 gcaaggtgcg cataccacgt aaaagtaacc atgcatcatg cggcgaaggc acagcaccga
     9901 atcccataaa tactctgcga aacatgcctg caatatgttc ggaattgcca caaactgcac
     9961 ctgctaccac atcgctatga ccggcaagat attttgttgc cgtatgaata acaaaatcaa
    10021 cgcctaattt atgcggatgc tgataaatgc ctgcggcata actattgtct attgctgtga
    10081 aaatatgatg ctttcttgca agcttggaaa cagcttcaag gtcttgtaat tcgaaccaga
    10141 aggtatttgg actttcgaga taaatgagtt ttgtatttga ttgaattgct tgttcaaatt
    10201 gagacatttc gcgtccatca ataaatgttg tcgtgatatt atactcagga aatacctcag
    10261 taagcaaata ttgcgtccag ctataaggac tcttcacaca tataacatga tcaccgctac
    10321 gaagtgtatg caataatata gcagcaatgg cagccacgcc actactgaac accagacaat
    10381 catcggtacc ttccaaggct gctatttttt ttcttacaat ttcaactgta ggattatttc
    10441 ctctggtata cacatgatgc tgttgctccg tattaatagc atcccgaaaa tcggcgacgg
    10501 ttttaaatgc aaaattgctg gtctgcacta tcggaggtgt taccgcatca aaatactgat
    10561 cgcgttcttc gccgaggata ttgaggatgt atgacaggtc catgatagtg ggttattgga
    10621 ctgtaaaagt aataaatggg tgagcttgcc tcgcctctgg cgggtgggtg agtgggtgag
    10681 tcgtgagtcg tgaatcgtga gtcgtgaatc gtgagtgtgg tatgtgaatc gtgaattgtg
    10741 aggttgcctc gcctctgcgg gtgggtgagt gggtgagtgg tgagtttgct tatttaccat
    10801 tcccgacccg actgctcggc gagaccagcc cgcgagtcac gattcacgag tcacgacttt
    10861 acattcccag gtgagactaa tgacgttgga tttgattggt attactcatt tcccataaat
    10921 aaaataaccc gccgagaggc gggctatttt ttcaggcata ctcaaagaaa agacaaccgt
    10981 atggcacgat tgatcgtgtc aagcgtcgtt ttaacagaac gatgaattac gacgcagtaa
    11041 ctttttttcc tttcatcatt gctttcaccg gacaccagcc gagaacagca gtaaggaaaa
    11101 ttcccataac aacaggtatc atccagtagg catgtaaaac gcctgctacg atgccaacta
    11161 ctagaagtaa ggcatacctt actatgattt cgtcgatttt aagatttgga ttaaagttca
    11221 tatacattgt gattacgttg gtaaagatca ttctaccaca aaccacaagt aatgacttcg
    11281 atcaggcaaa tcggtgattt tagtcagccg acagcgatta acatttttca ggcttgaaaa
    11341 agctttttca gcaaagtgtg cactgctaaa gaagggagca attgtccggt ttggatatca
    11401 gcttcagcag atttgagcca ttcgtggtat tgcggatgct cactcacaga gcgcagaata
    11461 gcataatgta attgatcttg aaaccagctt tcacgctgcc ggatgcgatt ggattcgaga
    11521 tatccattat gcttcgtcaa ctcaaaatac ttgtcgattt gatgccttaa ttcagggatt
    11581 cccctaccct caagacctga aatcaattca acacaaacag gccattcaga tgtcttcgac
    11641 tgaaacagat gtatagctct tctcacttct gctgctgcca gttttgcgcg ttctacatta
    11701 tcgccatcgc atttgtttat gaggatgatg tctgccagtt ccatgatacc tcttttaata
    11761 ccctgtaatt catcgcctgc acccggaatc aacaagagga taaaaagatc cgtcatgtcg
    11821 taaatggcag tttcgccctg acctactcca accgtttcta tcaatatcgt atcaaatcct
    11881 gcagcttcca gcaagatgat ggtttcacgc gtagccgctg ccacaccacc cacatgttcg
    11941 ccgctggctg ttggcctgat aaatactgag gcctcccgag atagctgctg catacgcgtt
    12001 ttatcgccca gaatacttcc cttgctgatt cggctgctgg ggtcaactgc cagtactgcc
    12061 aattttttat tgtcggcaag catttgcaat cccaaacttt caattgtggt gcttttacct
    12121 gctccggggc tacctgtaat accgatgcgc atggcagagc cactgagagg atagcatgcc
    12181 gacaataagg ccaatgcttg ctctcgatga gcaggtacag cgctttcaat caacgtaatt
    12241 gcgcgggata ataataccct gtcgccattg cggatacctg ttaaccattg ctctgttgtg
    12301 agatcgagtt gtgtcattac cggtataaaa ataagatccc cccgcgtaaa acacggaggg
    12361 accacaaaat attcttaatt gcttatcaga atggattaaa tgaaacaccc actgttatag
    12421 ggtttcctgt tggcccttcg ccatcggtga atatcggtgc caatccgtaa taggcttcga
    12481 tattgatctt gctgtagccg agtcggagcg ttggtccgta ttgcaaggta cttacatttt
    12541 taacgcgata ttgtttgaat ttgatctcat cgccggatcc actgatataa tcatcgccaa
    12601 catatttggt atgattactg aatgcgtatc ccgccttgaa accgatagca gccttgaatc
    12661 tgttgccatt tttatcatcg gtgcggaaac ggaattccaa tggcacttca acgtagttga
    12721 gtacaaattt atttttagaa tactgtaccg tatcagcaaa ttttacaggc acagtgtatg
    12781 atgatgtgtc gtaagtaaaa tatgaattgt tgtaataatt gctccatgaa acacctgctc
    12841 caatagcgaa gctgatatta tcatctttca atggcacatc gtacatataa tgcaacccga
    12901 taccacttga aaggcctttt attttcattg agtcaggagc acccatccaa ccatcccaat
    12961 gaacattgat tagaaatctg tcacgtgaag ggtcttcttt ttccaaaacc gttgttgttg
    13021 ttttcgctga atctacttgc gcaaatgccg tggcaatcgt aaaaaccatc acagccgcta
    13081 atatcaattt ctgcatgttg ctttgctttg taagtttatt gtatggggca aaattaacca
    13141 ttttcagtcg ttaacatggc ctttatgcac gtccgaagcc taataacgtc taaaatttgc
    13201 gcttggtatg gcaccttact ttggtttaac caggtaatcc cacgatatta tttatgccgc
    13261 cactttcccc tgaaaaagga tacatttggt tagttaaaat tgacacccga tgaaacagac
    13321 agttctactt ttaacatttg cttgcactct tggaacggca acagcgcaat ggagcgagat
    13381 ttttgaaacc gggaatattt tcggactcat cgatatttcg gcgcccaatg acaatattgc
    13441 ctgggcatgc ctttcttctg attcggcata tcgcacagca gatggtggac ttacctggga
    13501 tagaatccga cttcccgaat tcgagccgaa ttatcaaatc agtaatattt atgctttaga
    13561 tgagaatacc gcttttttat gcggtcaggt aatctttacc ggtgaaggcc ccggcattgt
    13621 atataaaact tccaatggag gtgtaagctg ggaaaatgtg tttcaggttg acagtaattg
    13681 caccttcacc atgcacttta agaatgaact ggaaggcatc atgacttgct tcacttctgt
    13741 atggtttggc ggcagcacat catttatgaa acgcactact gatggtggca atacctggac
    13801 aagtgatatc acagaacctg atgctgaatt ttatggattt actaatcaag gcatgtatgc
    13861 aaaaggggat tcaatttggt tatgtgatat cgatggcaat atgcattatt cagatgacat
    13921 ggcttcgtcg tggacccaaa tatttacggc tacattttcg aatgtattgc gcatgccggc
    13981 atttaaaaat tcagattacg gtatggtct
//
LOCUS       DAOQOU010000077        14006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2245_length_14006_cov_5.182711, whole genome shotgun sequence.
ACCESSION   DAOQOU010000077 DAOQOU010000000
VERSION     DAOQOU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_14006_cov_5.182711"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLM90_08080"
     CDS             complement(<1..809)
                     /locus_tag="PLM90_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMZ89370.1"
                     /translation="MTMRVYLDNAATTALDPQVLETMLPFLQQHHGNPSSIHSFGRET
                     RAAIEKARKQIAKILNVSPGEIFFTSGGTEANNMALQCSVRDLDVKHIITSPIEHHAV
                     THTVEALQSEGKIILSYVNILPDGSIDYAHLEQLLQLPNGKTLVSLMHANNEIGVLLD
                     MEKVSAMCIQYHALFHCDTVQTLAHYPLDLQATKVTFISCAAHKFHGPKGVGFIYING
                     DAAIKPLIHGGAQERNMRAGTENLYGIIGLAKALEIAYQELDTTRAHITALR"
     gene            complement(850..2232)
                     /gene="glmM"
                     /locus_tag="PLM90_08085"
     CDS             complement(850..2232)
                     /gene="glmM"
                     /locus_tag="PLM90_08085"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMZ89371.1"
                     /translation="MTLIKSISGIRGTIGGKAGHNLTPEDIIRFTAAFGTWIKRTTGN
                     PVIVIGRDARISGDMINHLVIGTLQSIGIKVVDLGLSTTPTVEIAVPRENAGGGIIIT
                     ASHNPKNYNALKLVNHLGEFISGTDGQEVLRIAEEEEISYSDVDKLGEYQHKFYYIQK
                     HIDKILELDLVNRKAIKERNFRVVIDCVNSTGGISVPELLVALGVTDVVELFTNPNGR
                     FPHNPEPLPEHLSTIAHEVKHQKADVGLVVDPDVDRLAIICENGDMFGEEYSLVAIAD
                     YVLQKRKGDVVSNLSSTQALRDVANKYKVAHHQSAVGEVHVVQKMKEVNAVIGGEGNG
                     GIILPELHYGRDALVGIALFLSHLATSGKSCTRLRAEYPNYFMAKNKIELPEGTNVQD
                     ILVQMEEKYKKEHLNKTDGLKIEMGSDWVHLRASNTEPIIRIYSESTTKTTAENINRK
                     IMDDIKMLMK"
     gene            2314..3054
                     /locus_tag="PLM90_08090"
     CDS             2314..3054
                     /locus_tag="PLM90_08090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ89372.1"
                     /translation="MTGNNTQKWYNSWFGTPYYDLLYGHRDEQEASAFVATLITYLHP
                     NTTATILDAACGKGRHAILLADKGFTVTGIDLSAKNIREAQAFENEHLSFFKHDMRQI
                     FRIGYFDLICNFYTSFGYFDDPKDDVACLKAFAAGLKPGGKLVIDFLNIYHILQHLIE
                     DESRTIDGIRFTISKSYSNGFLHKKITISDNGNFSEYHEKVRALELKQFERYLMEAGL
                     HLENIFGDYRLSPFEKSSSDRLILIARK"
     gene            3054..3641
                     /locus_tag="PLM90_08095"
     CDS             3054..3641
                     /locus_tag="PLM90_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ89373.1"
                     /translation="MFPEALQHIDEQLFLAINRQWGNQLFDTILPPVRDKMFWVPMYA
                     IIAVLIVYAWRWKGVLVVVCILANFALSDQVSSAVIKPAVGRLRPCNDPDFRDEVTLR
                     IDACGAGKSFTSSHATNTFAFAMICILLFRRRYKWVTPLALFWASMVSYAQVYVGVHY
                     PFDIIGGAILGVGMTIILYNIAIRYILKKYAPELI"
     gene            3665..4417
                     /locus_tag="PLM90_08100"
     CDS             3665..4417
                     /locus_tag="PLM90_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HMZ89374.1"
                     /translation="MTILQTILLIFAAIAGIGAAWILRKKTHSEFLSILLSFSGAYLL
                     GTVVLHLLPEIFHQEVSLLDHSNDAHITAVFVLAGFFIQLFIVQFTRGLEHGHMHLHD
                     HHTKSYVASVVFGLSVHAFMEGFPLSDAADSGVIEPVYKAILLHKIPEAFALATVLFF
                     SIRNKVITLSLLGLFTIMTPLGSYIGEVITSSHMLAYNRLIAIVCGSLIHISTTIIFE
                     ASGKAHKISLYKLGAILVGAGIALAGVWTESH"
     gene            complement(4414..9420)
                     /locus_tag="PLM90_08105"
     CDS             complement(4414..9420)
                     /locus_tag="PLM90_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HMZ89375.1"
                     /translation="MRRYLLTLTTIFFFIQAKAQTYGNEWIAYDQQYLKCYVAADGIY
                     RIDYNTLNTSLAGIGVSLSDIDPRHFQLFNQGEEQYIFIAGEADGSFDPADFIEFLGK
                     RNDGTFDTGLFDSAQFQLQTFESIISDTACYFLTWNDLFDNNRMAPAINDLAGAPSPA
                     PYFSAHSRIVFGSGYGSANFNAGPSYQNVYSSKYMNGEGFTSPKYNLSTYNVTVNTPN
                     RYAGVDGFAPTLSTIAIGSNEAEHHVVIKINASTMLDTTFSNYGVVRKTFLMPTITDA
                     NTVTFVSGPTTTDYQRYSFIDINYPRIFDFDNASRFRVDLPAIPGPTTYLEFNDMDEK
                     GTPVLLYDLAEKRRMIGITEADITKFHIPYPASLHKLYVTSQDPSDIKSITTLSPVTF
                     INYGDVANQGDFLIISHPSLFDDGTGTNYVAAYRDYRNSIDGGSYDARIANIDMLYDE
                     FSYGIRKHPLAIRNYILYAVDHYTDKPKYVFLIGKSFSYDVTRSNGTPEFYEDLVPTF
                     GHPGADALLAARPGSVVQTIPIGRIAAKTADEVRIYLDKVIAFEEAQHDNTQTVANKL
                     WMKNVLHFAGGLNEYEQALFNNYLNQYSYYIEDTLFGGNVRQFNKLSTDPIFYSESEY
                     IDSLINAGISLATFFGHSSTGSFDFNIGEPEDLNNAGKYFCVYGNGCNTAAIHGESLT
                     LGERYIFAEEKGAVAFIAASNFSLASSLNTYANMFYQEFSNYAYDLPLGDVLMRTTDT
                     LWPTGNIYQQLTLEHTTLQGDPSLRLNTHPQPDYDIEAPLVSFQPDIITPGIDTFYLE
                     LIVSNLGMAIDSSYFVEVKRTLPDGSEQTFFERFPSTYYQDTLHIPFATGGPDGVGIN
                     NFNIHVDKLNEIPEIDELNNVLGTSVTIISDDAIPIYPVEFSIMNHVPEYFAASTSYV
                     FAEEKLYLIEADTTMLFNSPLHQSTTALESGGVVYWNAPPMMWLDSTVYYWRITPDTS
                     DGSEPLWRSSSFEFRIGDITGWNQSHYHQFLEDDYTNIQLQPSRNFEFVPDVVTYEVS
                     TGIYPTTNWTEVTCYQDGELFAVSSCASAGFVVVVADANSGQPWTTSEVGTSNTGPYG
                     DIYCSADPYERVIQFNTNTPERREFLYQFMMNTVPDSSYFICYSNNYAEFNSWLDDTL
                     IYGHSLFDAFVAYGAVDINSLAVFDYDRSYIFYAQKGNPASKFELIGDPEGHKIEATF
                     VIEGAWNTGDIVTPLIGPAASWDKAQWWLSSYDDPQSDTSMLSILGIDTLGAMTVLAD
                     GLQSGDTSLTFIDAHTYPYLKLQLTASDDSLRTPVQLDRWRVIYDPVPEAALNPNIAF
                     SYDGDSLQQGPDVHLSMAVTNVSDYDMDSLLISVKVRDVANVLHPVPYERQDSLLTDE
                     TMIATLGFSSSDFPTGNNTLIVEVNPKNDQPEQYHFNNIAYLPFFNAADTRDPLLDVT
                     FDGIHILDGDIVSSKPTILINLKDENTFLALNDTSLIDIVIEYPDGSEHSFPYDDIIT
                     RFYPADSSDLAENNSAKVELQPVLEADGIYILKIHGEDVTGNNAGDLDYRISFEVINK
                     AMISNVFNYPNPFSTSTRFVFTLTGSEVPEYFKIQIITVTGKVVREIMRSELGELHIG
                     NNITEFAWDGTDQYGDNLANGLYLYRVVTRLDGKALENFETGTDDYFKSGYGKMYIAR
                     "
     gene            complement(9446..10501)
                     /locus_tag="PLM90_08110"
     CDS             complement(9446..10501)
                     /locus_tag="PLM90_08110"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMZ89376.1"
                     /translation="MSAAHTQTTAPKYSNEFLSIGVSAQALGMSNSVVAGVNDVTSAY
                     WNPAGLMLVEPDIQLGAMHAEYFAGIAKYDYGSVAIPLREKQAAIGFSLIRFGVDDIP
                     NTLYLVEPDGSINYDNITAFSVADYAFLGAYATKLKWQNLRIGGSAKVVHRQAGEFAT
                     AWGFGFDAGAQMDYKKWKFGVQLRDITSTFNAWSFSFTEEEKEVLTATGNSIPTNSLE
                     ITTPKMILGAAYDFSLGTKFGLRTELDADITTDGRRNVLLSANPISVDPHLGVEADYQ
                     DFIYLRAGIGNVQRYIPDDLTEEAWTLQPNMGLGIIIGPMKIDYAYSNIGKQAEVLYS
                     HVFSLRLDINKNIKSAK"
     gene            complement(10548..11222)
                     /locus_tag="PLM90_08115"
     CDS             complement(10548..11222)
                     /locus_tag="PLM90_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89377.1"
                     /translation="MNFLSHYYHELPCNDAYFVGGLILPDILSNYSYRAGRVVKLHPW
                     RLTEPKTAIQQSLTSGVKRHYAVDAAFHDSDYFTIQTRWIEDNLLSKPFDCFPRRKYA
                     IAHVMLEILLDRSLMVRFPDLTPGFYAKLEEIDDHAIAELMSNNGQEKDAVGVRAHFN
                     EFRRAAFLYDYLNDERLNALLARINRRLGNPLLSASDKQNLTTAIYDIEKAFLGEKFP
                     TFRTDS"
     gene            11378..12658
                     /gene="tyrS"
                     /locus_tag="PLM90_08120"
     CDS             11378..12658
                     /gene="tyrS"
                     /locus_tag="PLM90_08120"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMZ89378.1"
                     /translation="MKNFVEELRWRGMLFDMMPGTEEFLNKGMATGYIGFDPTADSLG
                     VGNMVQIMTLLHFQECGHKPIALVGGATGMVGDPSGKSAERNLLDEETLQKNIAAQKK
                     QIEKFLKFGNEGNAAEMVNNYDWMKDFSFLDFLREAGKYITVSYMMAKDSVQTRLERG
                     ISFTEFSYQLLQGYDFYYLWKNRGVMLQMGGSDQWGNITTGNEMIRRKGGGEAYAICT
                     PLIKKADGTKFGKTEGGNVWLDAKRTSPYKFYQFWLNASDTDAETYIKIFTLLDKTTI
                     DNLIEEHRAQPHLRLLQKTLAKDITIRVHSEEDYNNALAASDILFGKSTKADFEHLTE
                     ELLLDVLEGVPQFSIAANDLSAGLTANQILTEKTQVVSSKGEFKRALQENSLSINKEK
                     LSDGEMIITQADLIKGKFLLVQRGKKNYYLIIAQ"
     gene            complement(12645..13235)
                     /locus_tag="PLM90_08125"
     CDS             complement(12645..13235)
                     /locus_tag="PLM90_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89379.1"
                     /translation="MRNLILLLSFFTWQQVKAQGPFRDVTAAQFGDSILLNWTLTAGN
                     TCFDMHLQRSDGTTPFTTVFSVPGICGGTQDQYYSFVDYEDLKSGITYQYRLTASNDA
                     FISDSVLITYIDAGDRTLFVYPNPTKDHLELTIDNSLQPSFLIEVFDISGKRYSLSTY
                     PSNLVTLPTSDLSPGIYNLIVTTRDGQKFASDFIVQ"
     gene            complement(13274..>14006)
                     /locus_tag="PLM90_08130"
     CDS             complement(13274..>14006)
                     /locus_tag="PLM90_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HMZ89380.1"
                     /translation="QPFVANDVNINPTNLFNRLCSIAKAPFSVLYRFGEQYLICGSPE
                     RFFSYNGETMCSMPIKGTRRRGGDAVSDKRLRDELITSEKDRAEHVMIVDLVRNDLTP
                     YAIPGSITVDNLFGIYGFEQVWQMVSTIVARLRPDAHAVDALLQAFPMGSMTGAPKIR
                     AMQLAAQYEPFQRGMYSGTFGYFTPDGHCDFNVVIRSMLYNSEQQILAAFAGGAIVYD
                     SVAEKELEECLLKLSAIRRVLSDLP"
BASE COUNT         4087 a         2965 c         2947 g         4007 t
ORIGIN      
        1 cgcagagctg tgatatgtgc acgcgttgta tcgagctcct gatacgctat ttccaaagct
       61 tttgccaatc cgatgatacc gtataaattt tcggtaccgg cgcgcatatt gcgttcctgt
      121 gcaccgccgt gaattaatgg ttttatagct gcatcgccat tgatataaat aaaacctacg
      181 ccttttgggc cgtgaaattt atgcgcagcg caggaaatga aagtaacctt tgttgcctgc
      241 agatcgagtg gatagtgagc aagcgtttgc actgtatcgc agtgaaataa tgcatgatat
      301 tgtatgcaca tggcactcac cttttccata tcgagcagca cgcctatttc attattggca
      361 tgcatcaggc ttaccagagt tttaccattc ggcaattgca ataattgttc gagatgcgca
      421 taatctattg aaccgtcagg aagaatattt acataactaa gaatgatttt cccttccgat
      481 tgcagcgctt caacggtatg tgttacggcg tgatgttcta ttggcgaggt gatgatatgt
      541 ttcacatcca gatcgcgcac actacactgt aacgccatat tattggcttc ggtgccgccg
      601 ctggtaaaga aaatctctcc tggcgacaca ttcaatattt tggctatttg ttttctcgcc
      661 ttttcaatgg cagcgcgggt ttcacgcccg aaagaatgga tagacgaggg gttgccatga
      721 tgttgctgca agaatggcaa catggtctcc agcacctgag ggtcaagggc tgtggtggct
      781 gcattatcga ggtaaacgcg catggtcata ggtagtcaat tacaaaacta gcagaatttc
      841 agttcccgcc tatttcatta acattttgat atcatccatg atcttgcggt tgatattctc
      901 cgctgtggtc ttggtggtgc tctctgaata aatcctaatg atgggctctg tattgctggc
      961 gcgaaggtgc acccaatcgc tacccatctc tattttgagg ccatcggttt tattcaaatg
     1021 ctctttttta tacttttctt ccatttgcac aagaatatcc tgcacgttgg tgccttcggg
     1081 aagctcaatt ttatttttgg ccatgaagta attcgggtat tcggcacgca agcgggtgca
     1141 agatttaccg gatgttgcca ggtgcgacaa gaaaagtgca attcctacca atgcatcccg
     1201 accatagtgt aattcaggta atatgatacc gccattgcct tcaccaccga taacagcatt
     1261 tacctctttc attttttgca ctacgtgtac ttccccgaca gcactttgat gatgtgctac
     1321 tttatatttg ttggcaacgt cgcgcaaagc ctgagtactg gataaattgg atactacatc
     1381 gcctttgcgt ttttgtaata cataatcggc aatggctacc agtgaatatt cctcaccaaa
     1441 catgtcgcca ttttcgcaga tgatagcaag acggtcaaca tctggatcta caacaaggcc
     1501 tacatctgct ttttggtgtt tcacttcgtg cgcaatggta ctcaggtgtt cgggcagtgg
     1561 ttcgggatta tgcgggaagc ggccgtttgg attggtaaac agttctacca catcagtaac
     1621 accaagagca accaataatt caggaacaga aataccgccg gtagaattca cacaatcaat
     1681 taccacgcgg aagttgcgtt ctttaattgc ttttctattt accagatcga gttcaagaat
     1741 cttatcgata tgtttctgga tgtaataaaa tttgtgctgg tattcaccta gcttgtcaac
     1801 atcagagtaa cttatctctt cttcttccgc aatgcgaagc acttcctgcc catcagtacc
     1861 ggagataaat tcacccaaat gattcaccag tttgagtgca ttataatttt taggattgtg
     1921 ggaagcggta ataatgatac caccacctgc attttcgcga gggacagcaa tttcaactgt
     1981 tggcgtagtg ctcaagccga ggtctactac tttgataccg atagattgga gcgttcctat
     2041 caccaaatga ttgatcatat caccgctgat gcgcgcatct ctgccgatca cgatcaccgg
     2101 attgccggta gtgcgtttga tccaggtgcc gaatgcggcg gtaaatctga taatgtcttc
     2161 gggggtgaga ttatgccctg ctttacctcc gatggttccc cttataccgg agatggattt
     2221 tattaacgtc ataagtaacc ctgcaaaggt aatcaaaatc agtggcgacc gatacgtcgg
     2281 atagcgggat ttcggctatt tttgtccaca ccaatgacag gcaacaacac acagaaatgg
     2341 tataattcct ggttcggcac gccgtattac gacctgttat atggacatcg cgacgagcaa
     2401 gaagcatcag cattcgtggc aacactcatt acttacttgc atccaaacac gacagcaacc
     2461 atcttagatg cggcctgtgg taaaggtcgg catgcaatac tattggcaga caaaggattt
     2521 acggtaacag gtattgatct ctcagcaaaa aatattcgcg aagcgcaggc ttttgaaaat
     2581 gagcatctca gtttttttaa acatgacatg cggcaaatct ttcgcattgg atatttcgac
     2641 ctgatatgta atttctatac ttctttcggc tacttcgacg accccaaaga tgatgtggct
     2701 tgtttgaaag cctttgccgc aggattaaaa cccggtggaa aactggtaat agattttctc
     2761 aatatctatc atatcttgca acacctgata gaagatgaaa gtcgcactat tgacggcatt
     2821 cggtttacga tatccaaaag ttattcgaat ggatttttgc ataagaaaat cacgatttcc
     2881 gacaatggta atttttcaga gtatcacgaa aaagtaaggg cactggaatt gaaacagttt
     2941 gaacgatatc tgatggaggc cggattacat cttgaaaata tttttggaga ttatagatta
     3001 tcgcctttcg aaaaatccag ctccgaccga ttaatattaa tagcacgtaa ataatgtttc
     3061 ccgaagcact gcaacatatc gacgaacaat tatttcttgc catcaacagg caatggggca
     3121 atcaattatt tgataccata ttgcctcctg tacgcgataa aatgttttgg gtaccgatgt
     3181 atgccattat cgctgtattg atagtttatg cctggcgatg gaaaggtgtg ctggttgttg
     3241 tatgcatact ggcaaacttt gcgctcagcg atcaggtgag ttcagcagtt ataaaacctg
     3301 ctgtaggacg tttgcggcca tgcaatgatc ccgattttcg cgacgaagtc accttgcgca
     3361 tcgatgcatg cggtgcagga aaaagcttta ccagcagtca cgctacaaac acttttgcct
     3421 ttgccatgat atgcattctt ttgtttagaa gaaggtataa atgggtgacg ccgcttgcct
     3481 tattttgggc ttccatggtc agctatgcac aggtgtatgt aggtgtgcac taccctttcg
     3541 atattatcgg cggagcgata cttggtgtgg ggatgacgat aattctttat aacattgcaa
     3601 ttcgatatat ccttaaaaaa tacgcgccgg aactaattta gtgccgaaaa gattgtaata
     3661 aagcatgacc attctgcaga ccatattatt gatttttgct gccattgccg gaattggtgc
     3721 tgcctggata cttcgaaaaa aaacgcattc tgaattttta agcatactgc tttcttttag
     3781 tggcgcctat ctgcttggca ccgttgtttt acatttactg cctgaaatat ttcatcagga
     3841 agtaagcctc ttggatcatt ccaatgatgc gcatatcaca gcagtgtttg tgttggcagg
     3901 attctttatt caattattta tcgttcaatt tacacgtgga cttgaacatg gtcatatgca
     3961 cttgcatgat caccacacga aatcttatgt agcgagtgtt gtttttggat tgagtgtgca
     4021 tgcatttatg gaaggatttc cactttcaga tgctgcagat tccggtgtta ttgaaccggt
     4081 ttataaggcg attttattgc ataagatacc tgaggctttt gctttggcga cagtgctctt
     4141 cttttccatt cgcaacaaag tcatcacact tagtctgtta ggtttattca ccatcatgac
     4201 tccgctgggc agttatattg gcgaagtgat tacctcttcg catatgcttg cctacaacag
     4261 actaattgcc atcgtttgtg gttcactcat acatatctct accactatta tttttgaagc
     4321 aagcggtaaa gcccataaga tcagtttata taaactcggt gcaatcttgg taggagcagg
     4381 cattgcactt gccggtgtgt ggacagaatc acactaacgt gcgatataca tctttccgta
     4441 accggattta aaatagtcat ccgttccggt ttcaaaattc tctaatgctt ttccatcgag
     4501 acgagtaaca acacgataaa ggtataatcc attcgccaag ttatcaccgt attgatcggt
     4561 accatcccag gcaaattcgg tgatattatt tccaatgtgc aattcaccga gctcactgcg
     4621 catgatctct ctgacaacct ttccggtaac ggtgatgatt tgtattttaa aatattcagg
     4681 cacttcgctt ccggtgagtg taaacacaaa tcgtgtagag gtgctgaaag ggttcgggta
     4741 attaaataca ttgctgatca ttgctttgtt gatcacctca aatgaaatgc gataatccag
     4801 atcgcctgca ttattgcctg tcacatcttc gccatgtatt ttcaaaatat aaatgccatc
     4861 ggcctccaat accggttgca attccacctt tgcactattg ttttcggcaa gatcggaact
     4921 gtcggcagga taaaatctgg taatgatatc atcatacggg aaactgtgtt cactgccgtc
     4981 gggatattca attacgatat caattaaact cgtgtcattt aatgccagga aagtattttc
     5041 atcttttaag ttgatgagaa tagttggttt actgctcaca atatcgccgt caagaatatg
     5101 aatgccatcg aacgttacat ccagcaatgg atctctggta tctgcagcat tgaaaaatgg
     5161 caggtaagca atattattaa aatggtattg ctccggttga tcatttttag gattcacctc
     5221 aacaatgagc gtattatttc ctgtaggaaa atcagatgaa gaaaatccta aggtggcgat
     5281 catggtttcg tctgtcaaca aactatcttg tcgctcatac ggcaccggat gcaacacatt
     5341 tgctacatct cgcaccttaa cggaaatgag cagactgtcc atatcataat cactaacgtt
     5401 ggtcactgcc atactgaggt gcacatcagg gccttgttgc agactatcgc catcataact
     5461 aaaggctatg tttggattca gcgcagcctc aggaacggga tcatatatta ctctccatct
     5521 gtcgagttga acaggtgtac gcagtgaatc atctgaggcg gtaagttgca atttaaggta
     5581 aggataggtg tgcgcatcta taaatgtcaa agacgtatcg ccactttgca atccgtctgc
     5641 aagaacagtc attgcaccta atgtatcgat acccaaaata gataacattg atgtatcact
     5701 ttgagggtca tcgtatgatg aaagccacca ttgtgcttta tcccatgatg cagccggacc
     5761 gatcaatgga gtgacgatgt caccggtatt ccatgcacct tcaatcacaa atgtggcttc
     5821 tattttatga ccttcgggat ctccgatcaa ttcaaatttt gaagcaggat ttcccttctg
     5881 tgcataaaaa atataagatc tgtcataatc aaaaacagca agcgaattaa tatctaccgc
     5941 tccgtaagca acaaacgcat cgaacaaact atgtccataa atcaaagtgt catccaacca
     6001 tgaattgaac tcggcataat tatttgaata gcagataaaa taactactgt cagggactgt
     6061 attcatcatg aattgatata agaattctct tcgttcgggg gtattagtat taaattgaat
     6121 tacgcgttca taaggatcgg cactgcaata aatatcgcca taaggaccgg tattacttgt
     6181 tccgacttcg gaagtagtcc agggctgacc ggaatttgca tccgcaacca ccaccacaaa
     6241 cccggcgctg gcgcatgaac ttacagcaaa taattctcca tcctgataac aagttacctc
     6301 cgtccagttt gtagttggat aaatacctgt tgatacttcg tatgttacaa catcaggaac
     6361 aaattcaaag ttgcgagacg gttgcaattg aatattggtg taatcatctt ccaggaattg
     6421 atgatagtgt gattgattcc atcctgtaat atcacctatt cggaattcga atgaactgga
     6481 gcgccacaat ggctcagaac catcggaggt atccggggtg atgcgccagt aataaacggt
     6541 gctgtctaac cacatcatag ggggggcatt ccaatatact accccacccg attcgagtgc
     6601 ggttgttgat tgatgcaatg gtgaattaaa taacatggtt gtgtccgctt caataagata
     6661 caatttctcc tcagcgaaca cataggaagt cgatgccgca aaatactcgg gcacatgatt
     6721 catgatacta aattccacag gataaattgg aatagcatca tcagatataa ttgtcaccga
     6781 agtgcccagt acattattta attcgtcaat ttccggaatt tcattcagct tatcgacatg
     6841 tatattaaaa ttatttatac ccacaccatc aggtccgccg gtggcaaagg gaatatgcaa
     6901 agtatcctgg taatatgttg atgggaatcg ctcaaaaaat gtttgctcac taccatcagg
     6961 taaggtgcgt tttacttcaa caaaatagga agagtcgata gccatcccaa ggttgctcac
     7021 aataagttcg agatagaaag tatcaatacc cggagtgata atatcgggct ggaagcttac
     7081 taatggtgct tcaatatcat aatcaggctg cggatgcgta tttaaacgca atgacggatc
     7141 tccctgaagg gtggtatgtt ctaaggtgag ttgctggtaa atgtttcctg taggccataa
     7201 tgtatccgta gtcctcatca aaacatctcc caaaggcaag tcataggcat agtttgaaaa
     7261 ctcctggtaa aacatatttg cataggtatt caaactactt gccagcgaaa aattagaagc
     7321 ggcgataaat gcaacggcac ctttctcttc tgcgaaaata tatcgttcac caagtgtcag
     7381 gctctcacca tgaattgctg ctgtattaca gccatttcca tatacacaaa aatacttccc
     7441 tgcattattc agatcttcag gttcaccaat attaaaatcg aaacttccgg ttgaggaatg
     7501 tccgaaaaat gttgccaatg aaattccggc attgatcaac gaatcaatat attcactttc
     7561 gctgtagaaa ataggatcgg tacttagctt gttgaactga cgcacatttc cgccaaataa
     7621 agtgtcttca atataatagg aatactgatt caaataatta ttaaacaagg cttgctcata
     7681 ctcattcaaa ccgccggcga agtgcaacac atttttcatc cacagtttat tggcaaccgt
     7741 ttgcgtattg tcgtgttgcg cttcttcaaa tgctattacc ttatcaagat aaatacgcac
     7801 ttcatctgca gtttttgcgg ctatccttcc aataggtatt gtttgaacaa ctgagccagg
     7861 cctggcagca agtagcgcat cagctcccgg gtgaccgaaa gtaggaacta aatcttcata
     7921 aaactccgga gtaccgttag agcgcgttac atcataagaa aacgatttgc cgatgagaaa
     7981 tacatatttt ggtttgtctg tataatgatc tacggcataa aggatgtaat tgcgaatagc
     8041 caagggatgt tttctgatgc catagctgaa ttcatcatac aacatatcaa tattggcaat
     8101 acgcgcatca taacttccac cgtcaattga atttctgtaa tcgcggtaag ctgcgacata
     8161 atttgttccg gtgccgtcat cgaaaaggga agggtgacta ataattaaaa aatctccctg
     8221 attggcgaca tcaccataat tgatgaaagt aacaggtgat aatgtggtaa tagatttaat
     8281 atcgcttgga tcctgactgg taacatataa tttatggagc gatgcggggt aagggatatg
     8341 aaattttgta atatctgctt cagtaatacc aatcattctg cgcttctcgg ccaggtcata
     8401 aagcaaaaca ggtgttcctt tttcatccat atcattaaac tcaagatatg tagtaggtcc
     8461 gggaattgca gggaggtcca ctctaaaacg cgaagcattg tcaaaatcaa aaattctcgg
     8521 ataattgata tcaataaagg aatatcgttg ataatctgta gtagtaggtc cgcttacaaa
     8581 tgtcactgta ttggcatcgg tgatagtggg cattaaaaat gttttgcgga caacaccata
     8641 attactgaaa gtggtatcca gcatggtaga tgcattgatc ttaataacca catggtgctc
     8701 agcttcatta gaaccgatag caatggtgct gagtgttggt gcaaatccat cgacaccagc
     8761 atatctgttt ggcgtattga cagtaacgtt gtaggtagat aaattatact ttggtgaagt
     8821 aaatccttca ccattcatat atttagagct gtaaacattt tgatatgaag gtcctgcatt
     8881 aaaatttgca gacccgtatc cacttccgaa aacaattctg gaatgcgccg aaaaatacgg
     8941 tgccggcgat ggcgctcctg caagatcatt gatagccggt gccattcggt tattgtcaaa
     9001 caggtcgttc catgtcagga aataacaagc tgtatcactg ataatacttt caaatgtttg
     9061 caactgaaat tgtgccgaat caaataatcc ggtatcaaat gttccgtcat tgcgcttgcc
     9121 aagaaattca ataaagtcgg ccggatcaaa tgaaccatca gcttcacctg caataaaaat
     9181 atattgctcc tcgccctgat taaacaattg aaaatgcctg ggatcaatat cggaaagcga
     9241 aacaccaatt cctgctaacg aagtgttcag tgtattatag tcgatgcgat aaataccatc
     9301 ggcggctaca tagcatttga gatactgctg atcataagct atccattcat tgccataagt
     9361 ttgtgctttg gcctgaatga aaaagaatat ggtagttagc gttagtaaat atctcctcat
     9421 gcaggttgca ggttacatga aaaaattatt tggcagactt aatattttta ttgatatcca
     9481 gtctaaggct gaacacatga ctatataaca cctctgcttg tttgcctata ttcgaatagg
     9541 cataatcaat cttcatcgga ccgatgataa tgcctaatcc catattcggc tgaagagtcc
     9601 atgcctcttc agttaaatca tccggaatat agcgttgcac attgccaatt ccagcgcgca
     9661 aataaataaa atcctgataa tctgcttcaa cgcccagatg tggatcaaca cttatcggat
     9721 ttgcgcttaa caaaacattt cttctgccat ctgttgtaat atcggcatca agttctgtgc
     9781 gcagaccaaa ttttgtgccg agtgaaaaat catatgctgc acccaaaatc attttcggcg
     9841 ttgtaatctc caacgaattt gtaggaatgg aatttccggt tgctgtaagc acctcttttt
     9901 cttcttccgt aaatgaaaac gaccaggcat taaatgttga tgtgatatca cgcaattgca
     9961 cgccgaattt ccatttttta taatccattt gtgcaccggc atcaaagccg aaaccccagg
    10021 cggtagcaaa ttctcctgcc tggcgatgca ccacttttgc agatccgccg atgcgcagat
    10081 tttgccattt cagtttggtg gcataggcgc cgaggaaagc ataatcagca acagaaaagg
    10141 cagtgatatt atcatagtta atactaccat cgggttctac aagatacagg gtattaggga
    10201 tgtcatccac accaaagcga attagtgaaa atccgatggc cgcctgtttt tctcgcagtg
    10261 gaatagctac agaaccataa tcgtattttg caataccggc gaaatattcg gcatgcatgg
    10321 cacctaattg aatatcaggt tcaacaagca taagacctgc cggattccag tacgctgagg
    10381 taacatcatt gacaccggca accacactat tgctcatacc caatgcctga gcagacacac
    10441 caatggataa aaattcattg ctgtatttcg gcgccgttgt ttgtgtgtgt gctgccgaca
    10501 cgatgccaat actgagcagc aaaaggaagg tacatctcat ggtcaggtca cgaatccgtc
    10561 cgaaatgtag ggaatttttc gccgaggaac gccttttcaa tgtcataaat tgctgttgta
    10621 aggttttgtt tgtcggaggc tgaaagcagg ggatttccaa gccttctgtt gatacgtgcc
    10681 aacaaggcgt ttaaacgctc gtcattcagg taatcgtata agaaagcagc gcggcgaaat
    10741 tcgttaaaat gagccctcac ccctacggca tctttttcct gaccgttatt ggacattaat
    10801 tcggcaatgg catggtcatc aatctcttcc aacttagcat aaaatccggg tgtcagatcc
    10861 ggaaaacgga ccatcagact gcggtccaac agtatctcca gcatcacgtg ggcaatggca
    10921 tattttctgc gtggaaagca gtcgaaaggc tttgaaagca agttatcctc aatccatcgc
    10981 gtttgtatgg tgaagtaatc gctatcgtga aaggctgcat caactgcata atgacgtttg
    11041 actccggatg tgagcgactg ctgtatggct gttttgggct cggtcaatcg ccatggatgc
    11101 agttttacca cccgtcccgc ccgatagctg taattcgaca ggatatctgg caatatcaat
    11161 cctcccacaa aataggcatc attgcagggc aactcatggt aataatgcga aaggaagttc
    11221 atttagcaat ttaacggaaa aacagccgga aaggtttgct cctcgcggtg gatatcagcc
    11281 tttgggtgca agtttgattc aaatatgaca agtaaacagt acaagcggaa aatgggtcat
    11341 tttcagtaaa ataacaaatc ccgcctatct ttgctgcatg aaaaattttg ttgaagaatt
    11401 gcgctggcgt gggatgctat tcgatatgat gcccggaact gaggagtttt taaataaagg
    11461 aatggcaaca gggtatattg gttttgaccc gacggctgat tcattgggcg tgggcaatat
    11521 ggtgcagatc atgacattgt tgcattttca ggaatgtgga cataaaccca tcgcacttgt
    11581 tggtggagct accggcatgg ttggcgatcc gtcgggaaaa agtgctgaac gcaacctgct
    11641 ggacgaagaa acgcttcaaa aaaatattgc tgctcaaaaa aagcaaatag aaaaatttct
    11701 caaattcggc aatgaaggca atgctgcgga gatggtgaat aattacgatt ggatgaaaga
    11761 tttttcattc ctcgattttc tccgtgaagc aggaaaatat attactgtta gctatatgat
    11821 ggcaaaagat tctgtgcaaa cacgtctgga acgcggaatc tcatttacgg aattcagtta
    11881 tcagttgttg cagggttatg atttttatta cctctggaaa aatcgcggtg taatgctgca
    11941 aatgggtggc agcgaccagt ggggaaatat aacaacaggc aatgaaatga ttcgtcgaaa
    12001 aggtggtggt gaagcatatg ctatttgtac tccactgatc aaaaaagccg acggcaccaa
    12061 attcggaaaa acggaaggtg gcaatgtgtg gcttgatgca aaacgtacat caccgtataa
    12121 attttatcaa ttttggttga atgccagtga tactgatgcg gaaacttata tcaagatatt
    12181 tactttgctt gataaaacca ccatcgataa tctgatagaa gaacatcgcg cacaaccaca
    12241 tttgcgttta ttgcaaaaaa cactcgcaaa agatatcacc attcgcgtgc atagtgaaga
    12301 ggattataat aatgcccttg ctgctagtga tatattgttt ggtaagagta cgaaggccga
    12361 tttcgagcat cttacggaag aattattgtt agacgttctc gaaggtgttc cgcaattctc
    12421 aatcgccgca aatgacttgt cggcgggact tacagccaat caaatactta ctgaaaaaac
    12481 acaggtagta tcctcgaaag gtgaatttaa acgcgccttg caggaaaatt cattgagtat
    12541 caacaaggaa aaattatcgg acggtgaaat gataattaca caagctgatc ttatcaaagg
    12601 aaaattcctt ttagtgcaac gcgggaagaa aaattactac ctcattattg cacaataaaa
    12661 tcagaagcaa atttttgtcc atcgcgcgtg gtaacgatca aattgtagat acccggactc
    12721 aaatctgatg taggcaaagt aaccagattt gacggataag tagacaggct gtaacgcttt
    12781 cccgatatat cgaatacttc aattaaaaat gaaggctgca agctattgtc aatggtaagc
    12841 tccagatgat ctttcgttgg attcggatat acaaataatg tgcgatcacc ggcatcaata
    12901 taggtgatca aaacactatc actgataaat gcatcattgg aagcagtcag tcgatattga
    12961 taggtaatgc ccgatttcaa atcttcgtaa tccacaaaac tataatattg atcttgggta
    13021 cctccacaaa ttccgggaac agaaaatacc gttgtaaatg gcgttgtgcc gtcagaccgt
    13081 tgcagatgca tatcgaagca agtatttcct gcggtaagcg tccaattcaa taaaatacta
    13141 tcaccgaatt gagcggcggt gacatcgcgg aaagggcctt gagcctttac ctgttgccat
    13201 gtgaaaaacg ataatagcag tataagattg cgcatcgaag ggtataaaac gtctatagcc
    13261 tgattttagt atgttaaggc aaatctgaca acacacgccg gatggctgaa agtttcaaca
    13321 aacactcttc cagttctttt tctgccactg aatcatacac gatggcacca ccggcgaagg
    13381 ccgctaagat ttgttgctcg ctgttataaa gcatgctcct gatcacaaca ttaaaatcgc
    13441 aatgaccatc gggagtaaaa tatccgaaag tacctgaata catcccccgc tgaaaaggtt
    13501 catattgcgc cgccagctgc atggccctga ttttcggagc ccctgtcatg ctacccatag
    13561 gaaatgcttg caataaggca tccaccgcat gcgcatcagg tcgcaaacgc gcaacaatcg
    13621 tgctcaccat ctgccaaacc tgctcaaaac catagatgcc gaacaaattg tctaccgtta
    13681 tacttcccgg tatggcatat ggcgtaaggt cgtttcgcac cagatcaaca atcataacat
    13741 gctctgccct atccttctca ctagttatca attcatctcg taaccgctta tcactcactg
    13801 catctccacc ccttcttcgc gtgcctttga tgggcatact gcacatggtt tcaccattat
    13861 aactgaaaaa tctttcggga cttccacaaa ttaaatattg ctcaccaaat cgatataaaa
    13921 cagagaatgg agctttggca attgaacata accggttgaa taaattggtt ggattaatat
    13981 ttacatcgtt tgcaacaaat ggctga
//
LOCUS       DAOQOU010000078        14003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2246_length_14003_cov_4.799541, whole genome shotgun sequence.
ACCESSION   DAOQOU010000078 DAOQOU010000000
VERSION     DAOQOU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14003
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2246_length_14003_cov_4.799541"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLM90_08135"
     CDS             <1..877
                     /locus_tag="PLM90_08135"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HMZ89381.1"
                     /translation="NIYLHGGSYFYVLGTLDVQGTKDSVVTFQGDRLEDFYKDVPGQW
                     EGIYFLRGSTNNVIKYAEIKNALEGISLGFSTVPDLSSFLDNQVDLTLENTMIYDIQN
                     NGITSLNSVVKATNCLVYNIGASNVALFLGGDYQFRHCTLANYGSTYLSHQSPILGFT
                     DYFEFNIDNILQNDLTKADFYNCIIYGSIAEGDEIVIDTLLTPTVFNFNFDHCIMRTT
                     IDESLLNDTGCFYNQDPIFANIEERNYCPTTGSPALNAGIDLPWDPVLIDIRGEVRPF
                     AGTLPDIGAYETTAE"
     gene            951..1574
                     /locus_tag="PLM90_08140"
     CDS             951..1574
                     /locus_tag="PLM90_08140"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HMZ89382.1"
                     /translation="MLTFNVKHQESYSRGELLLRSFFGWLYILIPHGIAIMFYAIWIY
                     IATILAWWIILFTGKTPEFYYNAMLGFNRWGLRLSARIWNLSDGYPAIGPNGSDDKTE
                     LHYPMIHIGRGQLLLRSIFGFVYIIIPHVICLWFRMIATYFLVFLAWWTVLFTGKYPE
                     NWHKFNVGTLRWQMRLGLYYGWLLDKYPPFSGAPDQMEEAEKPIDQI"
     gene            1692..2120
                     /locus_tag="PLM90_08145"
     CDS             1692..2120
                     /locus_tag="PLM90_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HMZ89383.1"
                     /translation="MRRYCAYQERCHEEVRTKILSMKVYGDTLERVMDALIQEDFLNE
                     ERFAIAYAGGKFRMKKWGRLKILRELQLRKISTYCINKAMKEITDEAYEKTLKQLIRQ
                     ARKSYSTYNTFEQNHKVSKYLIAKGYEPALVWQYLGKDDH"
     gene            complement(2117..3982)
                     /locus_tag="PLM90_08150"
     CDS             complement(2117..3982)
                     /locus_tag="PLM90_08150"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HMZ89384.1"
                     /translation="MKATTLLGLFLLFSKGLFAQILLTGTVTDNQGIPVGFTTITTLQ
                     PVTQYTFANENGLYTLTFPDSMLNKRIQLVFNMIGYFPDTINVELNNKKIVQNKILTE
                     QILLESVVITSDNISGYAYGTTKIGKKGRKSKSPANGYTNTQSWSQVPITTAEEYHKS
                     PENNFKFVNENPVTTMSIDVDNASYSNIRRYLNLGELPPTDAVRVEEMINYFSYQYPK
                     PEEGQPYVVYTEEAPCPWNPEHRLLHVGFKGKTIEAENLPQTNFVFLIDVSGSMGDAN
                     KLQLVKSSMKLLVRNLRDEDRVAIVTYSSNVQVALQSTKCTHKDQILEVIDGLVASGS
                     TAGAAGIQKAYDIAEKNFINSGNNRVILCTDGDFNVGVSSPEELEKLIMINRDKGIFL
                     TCLGFGMGNLKDNKIELLADKGNGNYGYIDNIQEANKLLVETFGATMYTVAKDMKVQV
                     EFNPAYAAAYRLIGYENRVLKNEDFEDDAVDAGELGAGQTVTTLYEIIPAGSKDTLLK
                     NAPKLKYQKTDLTTEALNGEMATVKVRYKEPNGDTSIEAAYPVKISGVAFENTSTDYQ
                     FSAAVALYGMLLEQSQYCGKGDVSQVIKIAEQAKGEDIDGYRAEFVRLVKATPFN"
     gene            complement(4058..4519)
                     /locus_tag="PLM90_08155"
     CDS             complement(4058..4519)
                     /locus_tag="PLM90_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89385.1"
                     /translation="MKNITRNAEQVTVAVVILGLILRLLEIELADTILMIGLLTLSFV
                     YLMFGMLAINNNLQYLPIAQTGDTLTTVAKMISGLCGLAMSIAAIGIMFRILHWPSWQ
                     FMLMTGVVACAVCLLLCYLVVRPQHEKLASFLNYRMVMMLGMSLLLMYSSL"
     gene            complement(4519..4887)
                     /locus_tag="PLM90_08160"
     CDS             complement(4519..4887)
                     /locus_tag="PLM90_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HMZ89386.1"
                     /translation="MKSLYTIIFLIISNIFMTLAWYGHLQFKKLGWFENSGLFMIILL
                     SWGLAFFEYCFQVPANRIGSSTYGGPYNLFQLKILQEVITLVVFTVMALYVFKTETFR
                     WNYLAGFGCMILAVYFIFKK"
     gene            complement(5052..5603)
                     /locus_tag="PLM90_08165"
     CDS             complement(5052..5603)
                     /locus_tag="PLM90_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89387.1"
                     /translation="MSIVKILRTAILFLILEINAARGVAQSSDNFYVSPAFTIGYTFA
                     GGFAIGYDVDFGIWRDYMSAEREFNTGLSYHSLWTQAYYDRWHHIRSINFMMETDYYD
                     AKVGLGYVVDKHGYNHIVHCWAPGFYTDISFTDNEVYFPWIGMQGFWYNWSYFEGFEY
                     PYVTPYVKYKYDFLQQSGIKNFN"
     gene            complement(5845..6807)
                     /locus_tag="PLM90_08170"
     CDS             complement(5845..6807)
                     /locus_tag="PLM90_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMZ89388.1"
                     /translation="MKTLVIALLCISSFALHAQGVTTTTYYNLRYEQVPTKPDEGFIA
                     IITTPDSEKEHWADIKVTTADGILLQQYSYLNLDKGLRRDSIVYNRPDGTKVRRSYFD
                     ASGTLIREYAYDSLEQMTSSYEYEVNYLYSYEDGALESKTHVVNDKLTDSIYYYYPTG
                     KLRLIETFSSFGDYKSKISYHENGEVIYQSTFDENGNETVLIGEKPAEEAPAAKEGIQ
                     EFTEKMPEFPGGVSGLYAYISGHIQYPRAARDNGVEGTVYMKFVVTEDGAVIKAESLR
                     PIFPPMEFEALRVIMNSPKWTPGMMDGKNVAVWYRLPVKFTLED"
     gene            complement(6896..10435)
                     /gene="smc"
                     /locus_tag="PLM90_08175"
     CDS             complement(6896..10435)
                     /gene="smc"
                     /locus_tag="PLM90_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMZ89389.1"
                     /translation="MKLSTLEIKGFKSFADKTVLHFNENITGVVGPNGCGKSNVVDAI
                     RWVLGEQKPTALRTERMENLIFNGTKNRKSSGMAEVSLTFENTRNLLATEFSTVTVSR
                     HYFKTGESEYRINGVTCRLKDIQSLFMDTGISTDSYSIIELGMLDDILHDKDNSRRKL
                     FEQAAGISKYKSRKKETLNKLGATQLDLDRVEDLLFEIENNLKELEKQAKRAQRYQKL
                     KGEYKLNSIDLAVLTLQSHKDIYKQLSNQQTFESDAKLSIEAEIDALEASIADKKTAL
                     IDKEQSLSKLQKDLNAVVNEIHQKENDTNLSKERTKFLEEKQVSLQKSIEDAEQYIAS
                     TQGDIASLEQRVAEENEKLVGIKQSLQHFLESLEKVKQDHDQVREKLESTQSVFKQAE
                     RELYDIDKKIAINKVRLENYNRELGNIESDRGTQGDKLDPLRQELVAHDENRAAAQQT
                     VDALMKEDQDLKQTIAALQREIEKSRNEVGNARRKLDAKKNEFTLTKNMFESFEGFPE
                     SIKYLKKNVASMKESPLLSDVVSSQDAYKTAIESFLDPWLNYYIVQDMAHAMEAVNEL
                     ATKEKGRANFFLLSEFKSKQQPAAISGVVPALDVVTINEKYAALASHLLGNVYIIAAD
                     EDFAAVSARIASAHPDVHLLHQSGRVARSGKAISGGSVGLFKGKRIGREKTLETLEAE
                     IKTLQEEETLQSNALKEQQTKLSELQQSAVDRQIQQAREELNKVVQQQVSTKARIESI
                     EQLLQQMEDKKVAFMRQIQETDEESIKLNESHDKHMELKEKRQAELLDMEVLYREVAE
                     KLTFHSNQYNQENIKYHQQFNRLQSVQQDLDYKRTRRDEAMITAESSKKQLQDVTEEI
                     AENIEKVAVMQEALLKLYDEKTTMEQMVSREEENYYGSRGEVAETEDKIKALQKKKDS
                     SEHALGSIKDRLNELKVQLAGIKERLWVEFQVELESILDNEPPTDVTLEELQVKVDAA
                     KKRIENYGEVNPMAIQAYEEMNTRYDFIKTQREDLLQAKTSLLDTIKEIDETARNKFD
                     EAFMQIRENFKMVFRSLFNEEDDCDLLLKDDYDPLEAQIEIIAKPKGKKPQVIDQLSG
                     GEKTLTGMSLLFALYLIKPAPFCILDEVDAPLDDNNIMKFNNTIRKFADKSQFIIVTH
                     NKRTMSEVDIIYGVTMPEMGISKVVPVDFRGLN"
     gene            complement(10615..11862)
                     /locus_tag="PLM90_08180"
     CDS             complement(10615..11862)
                     /locus_tag="PLM90_08180"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMZ89390.1"
                     /translation="MKVFKFGGASVKDAAGLRNVGTIISSFQDKDIIIVVSAIGKTTN
                     ALEKIAVSAYEGGDYQEALQSLIAQHTGIAAELGLQNEFDFFGDMLVTLAGQNSHRSY
                     PQFYDQIVSIGELMSSSLLSQYLLSAGIANTWVDAREIIKTDNSWREGQVNWEETLKA
                     VQNTLPAALKSGHVVTQGFIGGAPDGFTTTLGREGSDYTAAILAHCTNAEGQWIWKDV
                     PGVLSADPKKYPDAVLLPELTYYEAIEMTYYGASVIHPKTIYPLQQKQIPFYVRSFLH
                     PEESGTVIATDNKFIPYPAVIMHKSGQTLISITPSLTTFVGDMQMANIYAIFSRHKLR
                     INTIQIAAQSVSIVVDYDAYLLESLMADLKASYSKVGVRENTDLQLLTVRHYNEDLLK
                     KLMGDKKVYLEQRSRSTVQFLYE"
     gene            12306..12617
                     /locus_tag="PLM90_08185"
     CDS             12306..12617
                     /locus_tag="PLM90_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89391.1"
                     /translation="MESNKKNGLANLTKKATAIFGAALAALTGIQANAIVPSQLDNLE
                     SKTIQVKEIGKVKPMPVLKLNPNNPENSVFVAQHSSHSSHSSHSSHSSHYSHRSGAMF
                     A"
     gene            12629..13015
                     /gene="hxsD"
                     /locus_tag="PLM90_08190"
     CDS             12629..13015
                     /gene="hxsD"
                     /locus_tag="PLM90_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HMZ89392.1"
                     /translation="MQFQNEIKDNEIIVFADTALYSKDSIFKCLYWYGDKFHTNLSFA
                     DSNTYRVSVKPISTMQLSSDELENLLLKVERDLVDFNLRDIVTKETQNVRDLLIAKAF
                     SNGEFDELPPGEVSDPVGFNVNQIRE"
     gene            13012..>14003
                     /locus_tag="PLM90_08195"
     CDS             13012..>14003
                     /locus_tag="PLM90_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ89393.1"
                     /translation="MIDARPDRKSRKFRDIENFDLNESNQYFLLPFRFHRVTAEKEII
                     VNEVGDYLILPNGTFERIVKRKISKEIDAELYGDLIANFFISEQAIPPLIDVLATRYR
                     TKKLFLNYFTGLHIFVISLRCEHTCHYCQVSRVTQNRDMYDMSIANIDKGIDIMFKSP
                     NSHVTMEFQGGEALLAFDNIKYAVEKAERIANELGKGLTKVICTNLAIVTEEILEYCK
                     EHKILISTSLDGPQYVHDKNRHKPHASSYELAVKGIELSRKVLGHDQVSALMTTTKLS
                     LDYPIEIVDEYFNLGFQGIFLRNISPYGFALRTDKSKYETDKFLTFYKKALDRI"
BASE COUNT         3932 a         2895 c         2832 g         4344 t
ORIGIN      
        1 aaatatttat ctccatggag gcagttattt ttacgtgtta ggcacattgg atgttcaagg
       61 aacgaaagat agcgtggtaa catttcaggg cgacaggctt gaagattttt ataaagatgt
      121 ccccggtcaa tgggagggaa tttatttcct gagaggcagc acaaataatg tcattaagta
      181 tgctgaaata aaaaacgcac tggaagggat atcacttgga ttttctacag ttccggattt
      241 atcatcattc ctcgataatc aggtagacct tactttggaa aacaccatga tttatgatat
      301 tcagaataat ggtatcactt ctttaaatag tgtggtgaag gcaactaatt gtcttgttta
      361 taatattggt gcttcaaatg tagcattgtt ccttggtggc gattatcaat ttcgtcattg
      421 tacgcttgcg aattatggtt ctacctattt gtcacatcag tcgccgatac ttggtttcac
      481 cgattatttc gaattcaaca tcgacaatat tttacaaaac gatctaacga aagcagattt
      541 ttacaattgt atcatatatg gttcgatagc agaaggcgac gaaattgtga tagatacatt
      601 attgacacca acggttttca atttcaactt tgaccattgt atcatgcgca ccaccatcga
      661 tgagtcgttg ttgaatgata caggttgttt ttacaatcaa gatcccatct ttgccaatat
      721 tgaagagcgc aactattgtc ccacaaccgg ttcgcctgcg ttgaatgccg gtattgatct
      781 tccctgggat ccggtgctaa tcgacatcag aggcgaagtc aggccatttg ccggaacgct
      841 tcccgacata ggcgcctacg aaaccacggc agagtagggg aataactcca ttaggaattg
      901 tcgttccgtt tgggtagatt tgattattgt tcatactcaa ataccctgtt atgctaacct
      961 ttaatgtaaa acaccaggag tcatattcac gaggtgaatt attgctccga tcattttttg
     1021 gatggttgta catccttatt ccgcatggca ttgccatcat gttctatgcc atatggattt
     1081 atattgccac tattctagct tggtggatta ttcttttcac aggtaaaaca ccggaatttt
     1141 attacaatgc catgctcggc tttaatcgtt ggggattaag attatcggca cgcatctgga
     1201 atctttcaga tggttatccg gcaataggtc cgaatggtag cgatgacaag accgaattac
     1261 attacccgat gatacatatc ggtcgcggac agttattgct gcgttctata ttcggttttg
     1321 tttatattat tattccacat gtgatctgtt tgtggtttcg catgatcgcc acttatttcc
     1381 ttgtgttttt ggcatggtgg accgtgcttt tcaccggaaa atatcctgag aactggcata
     1441 agtttaatgt aggtaccctg cgttggcaga tgcgtcttgg actatattat ggttggttgc
     1501 tcgataaata tcctccattt tcaggagctc ccgatcaaat ggaagaggcc gaaaaaccta
     1561 ttgatcaaat ttgattttag catctaaaaa agataaaccg gagtagttat gctccggttt
     1621 tttttgtgtc tttatatttg taggataatt gttttcgaat tagtgtcatg tacgaagaag
     1681 tgctaaataa aatgcgaagg tattgcgcct atcaggagcg atgccatgag gaggtgcgga
     1741 caaaaatctt gtcgatgaaa gtgtatggcg atacattgga acgcgtgatg gacgctttga
     1801 tacaggaaga ttttttgaat gaagagcgct ttgccattgc ttatgcggga ggaaaattcc
     1861 gcatgaaaaa atggggccga ctcaaaatac ttcgagagtt gcagttgcgt aaaatctcca
     1921 cttattgtat caataaagct atgaaggaaa ttacggatga agcgtatgag aaaacattaa
     1981 agcagcttat acgccaggct agaaaatctt attcaacata caataccttc gaacagaacc
     2041 ataaagtttc aaaatacctt attgcaaagg gatatgaacc tgcgctggta tggcaatatc
     2101 ttggcaagga tgatcattaa ttaaatggcg tagccttaac caaacgaacg aactccgccc
     2161 ggtaaccatc aatatcttca cctttcgctt gttcagctat ttttattact tgactcacat
     2221 ctccttttcc gcaatattgt gattgttcca atagcatgcc atacaatgcc actgctgccg
     2281 aaaattggta atcagtactt gtattctcaa aggcaacacc tgatattttt acaggatatg
     2341 cagcttcaat acttgtatcg ccattcggtt ctttatatcg cacttttaca gtggccattt
     2401 caccgttgag ggcttcggtt gttaggtctg ttttttgata ttttagttta ggtgcgtttt
     2461 tcaataaagt gtctttagaa cctgccggaa ttatttcata taaagtggtc accgtttgcc
     2521 ctgctcctag ttcccctgca tctactgcat catcttcaaa atcctcattt ttaagcaccc
     2581 tgttttcata accaattaac cgataggcgg cggcatacgc aggattgaat tccacttgca
     2641 ctttcatgtc tttcgccacg gtatacatgg tagcgccaaa agtttcaact aagagcttgt
     2701 ttgcttcttg tatgttgtcg atataaccat aattaccatt tcctttatcg gctagcaact
     2761 caattttgtt gtcctttaag ttgcccatgc caaaaccaag acaggtaaga aaaattcctt
     2821 tatcgcgatt aatcattatc aatttctcta attcctcagg gcttgaaacg cctacattaa
     2881 aatcgccatc agtacacaat ataacgcgat tgtttccact attaataaaa ttcttttctg
     2941 cgatatcata ggctttttga atgcctgcgg cacctgctgt tgatccagaa gctacaagac
     3001 cgtcaatcac ctcgagtatt tgatctttat gtgtgcattt tgtactctgc aatgcaactt
     3061 gaacattaga tgagtaggta acgatggcca ctcgatcttc atcgcgcaaa tttcttacga
     3121 gtaatttcat tgacgatttt accaattgta atttattggc atcacccata cttccggaaa
     3181 catcaatcag aaatacaaaa tttgtttgag gtaaattttc cgcttctatg gttttgcctt
     3241 taaatccaac atgcagcaag cgatgttcgg gattccacgg acaaggggct tcttcagtat
     3301 ataccacata aggctgacct tcttcaggct ttggatattg gtaagagaaa taatttatca
     3361 tttcctccac gcgcactgcg tctgtaggtg gtaattctcc taaattcaaa taccgacgaa
     3421 tattactata agatgcattg tcgacatcaa tagacatcgt agttacagga ttttcattta
     3481 caaatttgaa attgttttct ggactcttat gatattcttc ggctgttgta atggggactt
     3541 gagaccagga ttgggtattg gtatatccat ttgccggaga tttacttttt ctcccctttt
     3601 tgccaatttt ggtagttcca tatgcataac cagatatatt atcagaagtg attacaacac
     3661 tttccaaaag aatttgctca gtcagaatct tgttttgaac aatttttttg ttgttcaatt
     3721 caacattgat ggtgtccgga aaataaccga tcatattgaa taccagttgt atacgcttgt
     3781 taagcattga atccggaaag gtcagggtgt ataagccatt ttcattggca aaagtgtatt
     3841 gagtgaccgg ttgtaaagtc gtgatagtag tgaaaccaac ggggattccc tggttgtcgg
     3901 ttacggtacc tgtaagtaga atctgtgcaa acaggccttt gctgaaaagc aggaaaagac
     3961 caagtagggt agtggctttc atgatgtagg tttatgtatt aggaacgaaa cttttgataa
     4021 catgtttcat tccttttgtt atgaaattgt catttcgcta taaactactg tacatcagca
     4081 gcaaactcat tcccagcatc atcaccattc tgtagttaag aaaagaagct agtttttcat
     4141 gttgcggtcg aactactaaa taacataata gtaaacaaac ggcacatgca actacccccg
     4201 tcatcaacat aaattgccag cttggccaat gtagtatgcg aaacataata ccaattgctg
     4261 caatcgacat tgcaagtccg caaagaccgg atatcatttt cgcaactgtt gtaagtgtgt
     4321 caccggtttg ggcaattggc agatactgca agttattatt gatggcaagc atgccgaaca
     4381 ttaaataaac gaaactcaaa gttaataggc cgatcatcaa aatcgtatca gcaagttcaa
     4441 tttccagtag tcttaaaatc agtcccaaaa tgaccactgc aacagtgacc tgctccgcat
     4501 ttcgtgtaat gttcttcatt atttcttaaa gataaaataa actgccaata tcatgcatcc
     4561 gaatcccgct aaataattcc agcgaaaggt ttcagtcttg aatacatata atgccatcac
     4621 cgtaaacaca accagtgtga tcacctcctg taatattttg agttgaaata aattgtatgg
     4681 tcctccataa gtgctgctgc caatcctgtt cgcaggaacc tgaaaacaat attcgaagaa
     4741 tgccagtccc catgacagta aaataatcat aaacaacccg ctgttttcaa accatcccag
     4801 tttcttgaat tgtaaatgac cataccatgc cagcgtcata aaaatattgg agatgatgag
     4861 gaagataata gtgtatagtg atttcaagtt gtttgatttt ctgaatagct aagatagggt
     4921 gatagggtga gtgggtgagt gggtcagtgg gtgagtgttg tgtgggtgag cggatttttg
     4981 gggctggtat tgatatttca tttcaggaac tcaaaacatc gttagtttca ggcgtttgaa
     5041 gcgaagatag atcaattgaa attcttgatg ccggattgtt ggaggaagtc gtatttatac
     5101 ttgacataag gcgttacata gggatattcg aaaccttcga aataactcca attataccag
     5161 aatccctgca tgcctatcca gggaaaataa acttcattat ctgtaaagct gatatcggta
     5221 taaaatccgg gtgcccagca atgaacgatg tgattgtacc catgtttatc aaccacatac
     5281 cccaatccaa ctttagcatc gtagtaatca gtctccatca tgaagttgat cgatctgata
     5341 tgatgccagc gatcataata cgcttgcgtc catagtgaat gataagacaa cccggtattg
     5401 aattcccgtt ccgcgctcat ataatcacgc catatcccaa aatcaacatc atagccaatc
     5461 gcaaatccgc cggcaaaggt atagccgatg gtgaatgccg gactgacata gaaattatca
     5521 cttgattgcg caacaccacg ggcagcattg atttcgagga tcagaaacaa aattgcagtt
     5581 cgcaaaattt tgacaatgga cataggggag atttggattg agtgctttaa aatatgcaga
     5641 tgctttgatc accaaaattc cataatagga gaggagagag gaaaaaggag aaaggagaaa
     5701 ggtgaaagta gaaaggtaaa aggagaaagg tgaaagaaga aagtagaaag ctgaaaggaa
     5761 tgggatttat tgtctgtggg gggtgatgaa taggtgctaa ctttgctttt ccccgcgtga
     5821 cttccacatc tcatcgcgca ggcatcaatc ctccaatgtg aatttaacgg gcaggcgata
     5881 ccaaaccgca acattttttc catccatcat acccggtgtc catttaggac tgttcatgat
     5941 cactcttaat gcttcaaatt ccattggcgg gaatatcggt cgcaatgatt ctgctttgat
     6001 aacagcgcca tcttctgtaa ctacaaattt catgtatact gtcccttcaa caccattatc
     6061 cctcgcggcg cgtgggtatt gaatatgacc actgatatat gcatacaatc ctgaaacgcc
     6121 tcccggaaac tccggcattt tttctgtaaa ttcctgaatc ccttcctttg ccgcaggtgc
     6181 ctcctctgcg ggcttctcac cgatcagaac cgtttcatta ccattttcat caaaagttga
     6241 ttgatatata acttctccat tttcatgata cgaaatcttg gatttatagt cgccaaaaga
     6301 tgaaaatgtt tcgatcaatc tcagtttacc tgttggataa taataataga ttgaatcagt
     6361 cagcttatca ttcacaacat gtgttttgga ctccagtgca ccgtcttcgt aactatatag
     6421 atagttcact tcatattcat acgatgaggt catttgctcc aaagagtcgt aagcgtattc
     6481 acggatcaat gtaccactgg catcaaaata tgatcttctg accttagtgc catccggacg
     6541 attgtaaacg atagagtctc ttcgtaatcc tttatccagg ttgaggtaac tatattgctg
     6601 aagcaggatg ccgtcggcag tagtaacttt gatatcagcc caatgctcct tttctgaatc
     6661 aggggtcgtg attatagcga taaatccttc atccggtttc gttggcactt gttcgtaacg
     6721 caagttgtag taagttgttg ttgtaacgcc ttgtgcgtgt aaggcgaacg acgaaatgca
     6781 gagcaatgcg attactaagg tcttcattgg agatgatatt tgatttgcta aaataataaa
     6841 atgtaatgaa tgcaggttgc acgcaaaggc gctaagttgc aaaggtttac actaattaat
     6901 tcaacccacg aaaatcaaca ggcactactt tcgaaatacc catttccggc atggttacac
     6961 catagataat atccacttca gacatggtac gcttgttgtg cgtaacgatg atgaactgcg
     7021 atttatcggc aaatttgcgg atggtattgt tgaatttcat gatgttgtta tcatccaacg
     7081 gtgcatcaac ctcatcgaga atacagaatg gtgcaggttt aatcagatac agtgcaaaaa
     7141 gcaaactcat acctgtaagc gtcttctctc cacccgacaa ctgatctatc acctgtggtt
     7201 ttttaccctt aggtttcgcg ataatctcaa tttgcgcttc cagcggatcg taatcatctt
     7261 tcaataacaa gtcgcaatca tcttcttcat taaataaact gcggaatacc atcttgaagt
     7321 tttcacgaat ttgcatgaag gcttcgtcaa atttattgcg cgcagtctca tcaatttctt
     7381 taatagtatc taacagcgat gttttcgcct gtagtaaatc ttcgcgctgt gttttaatga
     7441 aatcataacg cgtattcatt tcctcatagg cctgaatcgc catcggattc acctcgccgt
     7501 aattttcgat gcgctttttc gcggcatcta cttttacttg taattcttca agcgtaacat
     7561 ccgtaggtgg ttcattatcc aaaatacttt ccaactcaac ctggaattca acccataatc
     7621 tttctttgat accggcaagt tgcactttca attcattaag cctgtctttg attgaaccga
     7681 gtgcatgttc actgctatct tttttcttct gcagtgcttt tatcttgtct tcagtctcag
     7741 ctacttcacc gcgactgcca taataatttt cctcctcgcg actcaccatt tgttccatgg
     7801 tggttttctc gtcgtaaagt ttgaggagtg cttcctgcat caccgcaact ttctctatgt
     7861 tctcggcaat ttcttctgta acatcttgta attgcttttt gctgctttca gccgtgatca
     7921 ttgcttcatc tcgacgcgtg cgcttgtaat caaggtcctg ctgaacactc tgcaatctgt
     7981 tgaattgctg gtgatattta atattttcct ggttgtattg attgctgtgg aaagtgagtt
     8041 tttctgcaac ctcgcgatac aatacctcca tgtcgagcaa ctctgcctgg cgcttttctt
     8101 tcagctccat gtgcttgtcg tgcgactcat ttaatttgat gctctcttca tcagtttctt
     8161 gtatctgacg catgaaagca actttcttat cctccatctg ttgcaataac tgctcgatgg
     8221 attcaatgcg tgcttttgtg gaaacttgtt gttgaacaac tttatttaat tcttcacgag
     8281 cctgttgtat ctgacgatca actgccgatt gttgtaactc tgataatttg gtttgttgtt
     8341 ccttcagcgc attactttgt aatgtttctt cttcctgtaa ggttttaatt tccgcttcca
     8401 gcgtttcgag tgttttctcg cgaccaatac gcttgccttt aaataaacca acactacctc
     8461 cggaaatagc tttacctgaa cgcgctacgc gaccactctg atgcaataag tgcacatcag
     8521 gatgagcaga ggcgatgcgt gcacttacgg cagcaaaatc ttcatccgca gcaataatgt
     8581 atacattccc caataaatgc gatgccagtg cagcgtattt ttcatttatg gtaacaacat
     8641 ccaaagcagg taccacgccg ctgatggcag caggttgttg cttagatttg aattcactta
     8701 acaggaagaa attcgcacgt cctttttctt ttgtcgcgag ttcatttact gcttccatgg
     8761 catgagccat gtcctgaacg atgtaataat tcaaccatgg atcgagaaaa ctttcaatgg
     8821 ctgttttgta tgcatcctga ctgctcacca catcgctcag caatggcgat tccttcatgc
     8881 tggcaacatt cttcttgagg tatttgatcg attcagggaa tccttcaaaa ctctcgaaca
     8941 tatttttggt gagcgtaaat tcatttttct tcgcatccag tttgcgacgt gcattaccca
     9001 cttcattgcg cgatttttct atctcgcgtt gcaatgctgc aatggtttgt tttaaatcct
     9061 gatcttcctt catcaaggca tcaacggtct gttgagcagc agcacgattt tcatcatgcg
     9121 caaccaattc ctgacgcaat ggatccaatt tatccccttg tgtgccgcga tcactttcaa
     9181 tattgcctaa ctcgcgattg taattttcaa gacgcacttt gttgatggca atctttttat
     9241 cgatatcgta caattcgcgt tcagcttgct taaatacgga ttgagttgac tccagttttt
     9301 cacgcacctg atcgtgatct tgtttcactt tttcaagcga ctcaaggaaa tgttgaagcg
     9361 attgtttgat gccgacgagt ttctcgtttt cttcggcaac acgttgttcc aatgaggcaa
     9421 tatcgccttg tgttgatgcg atatattgtt cagcatcttc aatactcttt tgcaggctta
     9481 cttgtttctc ttcaagaaat ttcgtgcgct ccttgctgag gttggtatcg ttttcttttt
     9541 gatggatttc attcacaaca gcattgagat ccttctgcaa tttactcagc gattgctctt
     9601 tatcgatgag ggcagttttt ttatccgcaa tgcttgcttc caacgcatct atctctgctt
     9661 caatagaaag ttttgcatca ctttcgaatg tctgctgatt actcagttgc ttataaatat
     9721 ccttatgcga ttgcagggtg agcacagcaa gatcgatgct gtttaattta tattcaccct
     9781 ttaatttctg atatcgttgg gcgcgttttg cttgtttttc gagttctttg agattgtttt
     9841 cgatttcgaa taacaggtct tccacgcgat caagatccag ttgcgtggca ccgagtttat
     9901 tcagcgtttc ttttttgcgc gatttatact ttgaaatacc ggcagcctgt tcaaataatt
     9961 tgcgacgtga attatcctta tcgtgcagga tgtcatccaa catacccaat tcaataatag
    10021 aataagagtc ggtgctgatt ccggtgtcca tgaagaggct ttgaatatcc ttcaggcgac
    10081 aagtaacacc gttgatgcga tactcgctct caccggtttt aaagtagtgg cgagatacgg
    10141 ttaccgtgct gaattcggtt gccagcaggt tgcgtgtatt ctcgaaggtg aggctcacct
    10201 cagccatacc tgacgatttc ctgttcttcg tgccgttgaa gatcaggttc tccatgcgct
    10261 ccgttctcag tgcagtcggc ttctgctcgc caagcaccca gcggatagca tccaccacat
    10321 tcgatttacc acagccgttt ggccctacca ccccggtgat gttctcgttg aagtgcagaa
    10381 cagtcttgtc tgcaaaactc ttgaaccctt tgatctctaa cgttgaaagt ttcatagtct
    10441 ttaagcgggt caaaaataaa tgaacgcagc acccacgtca aaaatgtgcc gatatttgta
    10501 ttaacaattg tggataaaat ggatttgata aaggtgtatg cctttcaaaa cctgtgcaaa
    10561 tgcctcccca aaacagtatt gaaaattgga aataatatgg tctgctaaac gcaattattc
    10621 atacaagaat tgcactgtgg aacgagaccg ttgttcgaga tagacttttt tgtcgcccat
    10681 caatttcttc aacaggtctt cattatagtg ccttaccgta agtaactgaa gatcagtgtt
    10741 ttcgcgcacg ccaaccttgc tatacgaagc cttcaggtcg gccatgagtg attccaacag
    10801 gtacgcatcg taatccacca caattgatac gctctgtgcg gcaatctgta tggtattgat
    10861 gcggagttta tgcctggaga aaatggcata gatatttgcc atctgcatat cgccgacaaa
    10921 tgtggtgagc gaaggggtga tggagatgag cgtttgtccg cttttatgca tgatcactgc
    10981 cgggtagggg atgaacttgt tatcagtggc gatgaccgtg cccgattcct ccggatgcag
    11041 gaaagaccgc acataaaacg gtatctgctt ctgttggagt gggtaaatgg tctttgggtg
    11101 gatgacactg gcaccgtagt aggtcatctc tattgcctca taataggtca gttccggcaa
    11161 caggaccgcg tcgggatact ttttcgggtc tgcactcagc actccgggaa cgtctttcca
    11221 tatccattgg ccttcagcat ttgtacaatg ggcgaggata gctgctgtgt agtcagagcc
    11281 ttctctgccc aaagtggtag taaaaccgtc cggagcgcct ccaatgaagc cctgggtcac
    11341 tacatgaccg ctttttagcg ccgccgggag ggtattttgc actgctttaa gcgtttcctc
    11401 ccagttcacc tgcccttcgc gccaggaatt gtcggttttg atgatttcgc gtgcatcaac
    11461 ccaggtattg gcgatgccgg ccgacaagag gtattgactc aaaaggctgc tcgacatcag
    11521 ctcgccgata ctcacaatct ggtcgtagaa ctgagggtag gagcggtgac tgttttgccc
    11581 cgctagcgta accagcatat caccaaagaa gtcgaattcg ttctgcagtc ccagttctgc
    11641 tgcaatgccg gtatgctgcg cgatgaggct ttgaagtgct tcttggtagt cgccaccctc
    11701 gtaagcgctt acagctatct tttccagcgc attggtcgtt tttccgatcg ccgacaccac
    11761 gataatgata tccttatcct gaaaactgct gataatggtt cccacattcc gcaaccctgc
    11821 agcatccttc accgaggccc caccgaattt gaacactttc atggtgtaaa ggtaaaaagc
    11881 aaaagtgata gggggttgaa gagtagaaca gccgtgtata ggttgggggg aaattagtat
    11941 ttcagaggaa taagcttaat tgtgacataa caaaccacct cgaattgcat cataaacggc
    12001 cggaggagag gtggaatggg ggggtgtggt tttgattggg gatgtttttg tgacatggag
    12061 ggattaacgg ttttccgtga atataaaaac atgagagaag tgtaaatttg taagacgtaa
    12121 tccgcataaa aattaacatt tgtttcgctt tagcatctgt tttggggtgg ttggcgaaat
    12181 ggggttgcgg ctataagtaa gttatgcctc atgctattgc gaccgaaaca cgaccgaaaa
    12241 aatcacgaca ataaaagaac aactaaacag aaatttatta tctttgaacc gttaaattaa
    12301 caaatatgga aagcaacaaa aaaaatggac ttgccaactt gacgaagaag gcaaccgcaa
    12361 ttttcggagc agcattagcc gctttgacag gtattcaagc aaacgctata gtaccatcac
    12421 aacttgacaa tcttgaaagc aagacaattc aagttaaaga aattggaaag gttaagccta
    12481 tgccagtttt gaaactaaac cctaacaatc cagagaatag tgttttcgta gctcaacaca
    12541 gctcacactc ttctcatagt tctcacagct cacatagttc ccactactct cacaggtcag
    12601 gagcaatgtt cgcttaaatc attctgttat gcaatttcag aatgaaatca aagacaatga
    12661 aataattgtt tttgcggata cggctttgta ttcaaaggac agcattttta aatgccttta
    12721 ttggtatggc gacaagttcc atactaattt gtcatttgca gacagcaata cataccgagt
    12781 ttctgtaaaa ccaatctcta caatgcaact ttcctcggac gagttagaaa acttgttatt
    12841 gaaagttgaa agagatttgg ttgatttcaa tttgcgtgac attgtgacga aagaaactca
    12901 aaacgtaaga gacttgctaa ttgctaaagc attttcaaac ggtgaatttg atgaactacc
    12961 tcctggagaa gtttcagacc ccgtaggttt taatgtaaat caaattaggg aatgatagat
    13021 gcaagaccag acagaaaatc acgaaaattt agagatattg agaacttcga tttaaacgaa
    13081 agcaatcaat attttctact tccatttcgt ttccatagag ttacagccga gaaagaaata
    13141 atcgttaacg aagttggcga ctatctgatt ttaccaaatg gaacatttga gagaatagta
    13201 aaacgaaaaa ttagcaaaga aattgatgct gaactttacg gagacctaat tgcaaatttt
    13261 ttcatttccg aacaagctat tcctccatta attgatgttc ttgcgactcg atacagaaca
    13321 aaaaaactat tccttaatta ctttactgga cttcacattt ttgttatctc tttaaggtgt
    13381 gaacatactt gtcattattg tcaagtgtca cgtgttaccc aaaacagaga tatgtatgat
    13441 atgtcaatag ccaatattga taaaggcatt gatataatgt ttaaatctcc gaactctcat
    13501 gtaacaatgg agtttcaagg cggtgaagca ctattagctt ttgacaatat aaaatatgca
    13561 gttgaaaaag ccgaaagaat tgcaaatgaa ttaggaaaag gacttacaaa agttatttgc
    13621 accaatcttg caattgtaac agaagagatt ttagaatact gcaaagaaca taaaatactt
    13681 atttcaactt ctttagatgg gcctcaatac gttcacgaca agaatagaca taaaccacac
    13741 gccagtagtt acgaattagc agttaaggga attgaacttt cgagaaaagt tcttggacat
    13801 gaccaagttt cagcccttat gacaacaaca aaactttcgt tggactatcc aattgaaatt
    13861 gtagatgaat attttaattt agggtttcag ggtatttttt taagaaatat cagtccttat
    13921 ggcttcgccc taagaactga taagtcaaaa tatgaaaccg ataaatttct tacattttat
    13981 aagaaagccc tcgatagaat ttt
//
LOCUS       DAOQOU010000079        13981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2250_length_13981_cov_6.493896, whole genome shotgun sequence.
ACCESSION   DAOQOU010000079 DAOQOU010000000
VERSION     DAOQOU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13981
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_13981_cov_6.493896"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLM90_08200"
     CDS             <1..810
                     /locus_tag="PLM90_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89394.1"
                     /translation="CNSIDDDCDASIDEGVIVATITPSGPTSFCNGSTVTLSANTGAG
                     YTYQWIRNGANVAGATSANYTVNKTGNYQVKITVPGGCFNTSATTNVTVFAAPKATIT
                     APGGLDLCGTGSVMLKANLGTGYTYVWKKNGVVIAGETTNVYIATTTGNYRVTVTNAS
                     GCSKTSANTTVVQTCRNAVPIVGDETMEVFPNPASDIVHIHVSGIEGIGEIAKIIVSD
                     LTGQQMIVQDALIIDGDIDIDLKTNELPSGVYLVTVNGAEAQLSQQLVISR"
     gene            980..3256
                     /locus_tag="PLM90_08205"
     CDS             980..3256
                     /locus_tag="PLM90_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ89395.1"
                     /translation="MFGIFLATSVIGQDTLFQESFDYPALFPPMGWTNVKVAGTGAPG
                     TWKRVTTGGSPIQEPHSDPAEAEFNSYFYPSGTASDLATPVIDFTVSGTNLVSFWMYR
                     DAGVGTDKMEVYVNTTQASAGGTLIGTVNRNYLATPVEPAAGWYQYTFTVPSTYNTAT
                     NYVIFKGVSGYGYNIFIDDIAILRDPVVTIPGCATSIFPANGSIDICPNLTVYWDVVP
                     YATGYKITMGNNPPNYNNVASNLDLGNALSFTAMLATGSNYKWKVKPYNVTGIAGGCI
                     NNSFTTATTPCYCEADFIEPSCSSLDFIDDVYTTGAVTNFSNMNTGCSPNPGNYTYYS
                     GTTVSANQGSTFTMNLQSGPDYPEGFAVWIDWNRDGDFNDADEYALSSAPTTSLVSNV
                     INVPATAPLGTTRMRIRAFYNLTPPANMYCDLWNEGETEDYNINVTTCTFVTYYQDAD
                     ADGYGNAAVTTTSCTGAPAGYTADNTDCNDAAFSVHPGATELCNGIDENCNVTIDDNA
                     AVAIITPSGPTTVCKGASLILNANTGVGYTYQWNRNGANLAGATGSSYSVTKSGNYKV
                     TVTVPGGCTATSTVTTCTVNANPTSTISTPEGTNLCGLPDLDLVANAGAGFTYIWYKN
                     GVIMPGINTQINTVNTIGDYRVKVANAAGCSKTSAIKTVFTSCKEGASIAQGELYPNP
                     NNGQMTLSFFSPSILEGRLNVQILDLTGQVVFNEMKDLSNGSYAGTINASNLAAGLYL
                     VKMSAGDTQIQQQVIIAK"
     gene            3775..5634
                     /locus_tag="PLM90_08210"
     CDS             3775..5634
                     /locus_tag="PLM90_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ89396.1"
                     /translation="MKRLHTLLLAVVVACSSTAVHAQIYSYTNDLSGTPSGIAANATG
                     TNLSRINGTLETTACPDGFNSSQWSKSTLLTPGKPAVEFSATPDAGFQLDVTSISADI
                     RINPKGPTIWRIAYSADGGATWITNPSDIIVESTTCDFNTNISFDVDDFSTTNTVMVR
                     LYGLGAHSSLNGKAVIRNAMIDGSVVDQDLDGDGYGFLTDCDDTNPDINPGATEICNG
                     MDDNCNGDIDEGLTFTTYYADMDGDGYGDAGMSATTCDGTPDGYVMDATDCNDADAAV
                     NPGATEICNDVDDNCDGNIDEGLAVVWYADMDGDGYGDATMMLEDCAMPEGYVMDATD
                     CNDADAAVNPGMTEVCGNGIDDNCDGNIDESDVAASFEVIGDNPTCEGTGTYFMSTAT
                     GMDITYQWYRNMMPIAGATGATYTPANAGYYQLEVSNGVCSDMTDTVHVIIKQAPEPE
                     LYMELGTDVCVNGFVKIKVIPNPGTGSTYVWYYNGDVLAGATTELVETTDAGTYYCVV
                     TGPNGCSGTSEVVTVTADCRLAGTENGTLAVYPNPAKGNFTMQLNNAPVNGEAVVHIT
                     DMTGRVVYAVNTTIVDGQSQMNVSLENNIASGMYMVTVQTQHQIWNAQISIVK"
     gene            complement(5706..6233)
                     /locus_tag="PLM90_08215"
     CDS             complement(5706..6233)
                     /locus_tag="PLM90_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89397.1"
                     /translation="MNVRNVIISFLLVTGISAKAQPFADAVSYNDYVVNIQTTIAVAL
                     NEFNQSILVEGSTRETVEPFYNNLITVTNNSLSLIKAMPAYNGDSTLRDAASELFEFY
                     HSCFTNEYATVMTIVFDPNMTMDDYNRMNNILTDVSTRESVYDLSFQTAQYLFAIKNG
                     FTLEENDMQKTIDDN"
     gene            complement(6334..6675)
                     /locus_tag="PLM90_08220"
     CDS             complement(6334..6675)
                     /locus_tag="PLM90_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMZ89398.1"
                     /translation="MEVLTSPITLTESAMREIRNIISEKQIPAEYALRVGVKGGGCSG
                     LSYILGFDLQKEQDELYQIDDIRLVMEKSHGMYLAGMEIDYVEGLNNRGFSFNNPNAK
                     KTCGCGTSFEA"
     gene            7058..>7740
                     /locus_tag="PLM90_08225"
     CDS             7058..>7740
                     /locus_tag="PLM90_08225"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HMZ89399.1"
                     /translation="MRKFNYLLACLAMVLSFTAARAQLYNYTTDLGGAPASVAANTSG
                     TSLIRVNTAQAITGCPDGFNSNRFSESTTFLASRPAVEFSISPNSGYQLNITSLSVDA
                     RRNTKGPVTWRLAYSVDGGASWINNGSDYSVTVTSCGSANTLTWDIPDFSVPYTVYVR
                     VYAYGALSNLNGIATMKNIVLSGSTSFADVDADGYTSNVDCDDFNAAVNTGATEVCNE
                     IDDNCDGNI"
     assembly_gap    7741..7840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7841..9424
                     /locus_tag="PLM90_08230"
     CDS             <7841..9424
                     /locus_tag="PLM90_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ89400.1"
                     /translation="DDNCDGNIDEGVQSTFYADADGDGYGDAGATTMACSAPAGYVAD
                     ATDCNDANGSINPGASEVCNGIDDNCDGNADEGLTFDTWYADADGDSYGDAGSSVSTC
                     DGAPAGYVGDATDCNDADGAVNPGATEICNEIDDNCDGNIDEGVQSTFYADADSDGYG
                     DAGSTTMACSAPAGYVADATDCNDADGAINPGATEVCNGIDDNCDGNTDEGVLITFYA
                     DADGDTYGDAAVSTDACSAPGGYVANSGDCNDASNTVYPGAAEICANGIDEDCDGVVD
                     LPATIAAGGPTSICLGSSVTLTCTTGGTGLSYQWQKNGNPIAGATGVSYVATLQGNYR
                     CVVSKPSCSATSSPITVTVLLNPSANITTPDGLDLCGKAYVRLKANTGAGLTYQWYKD
                     GNAIGGATAVTYFATATGAYYVRVTNANGCSTNSATVNVFSSCRMAEGALAGDVNIMP
                     NPSNGKFTINFTTAQAMDASAQVSILNLAGQQVAGFEGQLIGGQMTAGVDIAPVAGAY
                     VVVINVNGVIFTEKLMIAE"
     gene            9614..10144
                     /locus_tag="PLM90_08235"
     CDS             9614..10144
                     /locus_tag="PLM90_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89401.1"
                     /translation="MELFWQILGYIIPALVVFGTTYMVIRAFLDREHKMRLLEFRQAH
                     LREALPVRLQAYERLTLFIERISLNNLLPRVRKQEMNVSEFRTALINNIRLEYEHNLS
                     QQVYVSVDAWNMIRAAKEETISVINRNAMSMLPELPGVELHKKIIAELAESEEEATSQ
                     KVLVQLKKEVMQLYGK"
     gene            10240..10998
                     /locus_tag="PLM90_08240"
     CDS             10240..10998
                     /locus_tag="PLM90_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ89402.1"
                     /translation="MHKRNSILRLLLLLAIASTLGACQHYYTDARYDMQQMRVDNLVP
                     DSATASIIAPYKNVLDAQMGAVVCTVGIDLNRAKPESLLGNAICDMMVSEVARITGEA
                     INFGVYNYGGIRQEFLNKGPLTTGKVYELLPFENFGAVVTMSGTEVAQLVQKIIDEGG
                     WPVSGGIKIIITDNKASQVFINGNLLDPNGTYRVAMNDYMANGGDNLSFIKKENVVVL
                     GTTIRDMFLHYAATLQSEGKVLESSIEGRIQYDN"
     gene            10988..11920
                     /locus_tag="PLM90_08245"
     CDS             10988..11920
                     /locus_tag="PLM90_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HMZ89403.1"
                     /translation="MTTRRAFIQKSVTGTALFGLGALSLESFASDHIQKLTILHSNDV
                     HSRIDPFPDDDKKYPGMGGMANKAALISKIRAQEDNVLLLDAGDIFQGTPYFNFYHGE
                     LEMKLMTAMGYDAGTMGNHDFDAGIDGFAAQLPHAGFPILVANYDFTDTVLQDKVLRY
                     TTFQKKEIKVGVFGLGIELAGLVPGSLYAQTKYLDPVIIAQEMANVLRYDEKCDYVIC
                     LSHLGYSYKDDKISDIKLAQTTEGIDLILGGHTHTFLEEPLKYKNLADQEVIVNQVGW
                     AGVWLGRIDIFFERKKGKKWHSGKPVMVSKKTSE"
     gene            12000..13436
                     /gene="dnaA"
                     /locus_tag="PLM90_08250"
     CDS             12000..13436
                     /gene="dnaA"
                     /locus_tag="PLM90_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMZ89404.1"
                     /translation="MSSKDHQSVWASCLKIIKDNVNLQSFKTWFEPIEPVKLEDDVLT
                     IQVPSQFFYEWLEEHYVTLLRKTIKRELGVNARLEYRIVVENSSGNNSPFTIDYPNYN
                     TGANKNPEIAAPLVMGTSIKNPFVIPGLKKVNIESGLNANYNFDNFIEGDCNRLCRSA
                     GYAVAQKPGGTAFNPLVIYGATGLGKSHLAQSIGNEVKQNFPNKTVLYTNAERFTNQF
                     IESLKNNSINDFVHFYQLIDVLIIDDIHFFANKAKTQDIFFHIFNHLHQESKQIVLTS
                     DRPPRDLEGVEERLLSRFKWGLSADLQAPDFETRVAILEKKMYADGIELPKDVVEFVA
                     YNITNNVRELEGALVSLLAQASLNKKQIDLDLAKKIVKNFVKNMSREVSIDFIQKAVC
                     DYFEVPVEKLKEKTRKRQVVQARQLSMYLSKNFTKHSLKAIGKHFGGRDHSTVIHSCQ
                     AIQNLMDTDTKFRESVDDLQKKIQMSIS"
     gene            13604..13692
                     /locus_tag="PLM90_08255"
     tRNA            13604..13692
                     /locus_tag="PLM90_08255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13638..13640,aa:Ser,seq:gct)
     gene            13716..13790
                     /locus_tag="PLM90_08260"
     tRNA            13716..13790
                     /locus_tag="PLM90_08260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13751..13753,aa:Pro,seq:tgg)
BASE COUNT         4087 a         2740 c         3043 g         4011 t
ORIGIN      
        1 tgtaatagca tagatgatga ttgcgatgca tcaattgacg aaggtgtgat tgttgcaaca
       61 ataactcctt ccgggcctac atcattctgt aatggaagca cagtaacatt gagtgcaaat
      121 acaggtgcgg gatataccta ccaatggata agaaatggtg ctaacgttgc tggagcaaca
      181 tctgctaact acacggtaaa caaaaccgga aattatcagg tgaaaatcac cgttccgggt
      241 ggatgtttta atacatctgc aacaacaaat gttactgttt ttgctgcgcc gaaagcaacc
      301 atcactgcac ccggtggcct tgatctttgc ggaacaggaa gcgtaatgct taaagccaac
      361 ttaggcacgg gctataccta tgtatggaaa aagaatggcg tggtgattgc cggtgagaca
      421 actaacgtat atatcgcaac cactaccgga aattatcggg taacagttac aaatgcatca
      481 ggctgttcta aaacttcagc taatacaacc gttgtgcaaa cctgtcgcaa tgcagttccg
      541 attgttggcg atgaaaccat ggaagtattc cctaaccccg ccagcgatat cgtgcacata
      601 catgtatcag gaatcgaagg aattggcgaa atcgcaaaaa tcatagtaag cgatcttacc
      661 ggtcagcaaa tgatcgttca ggatgcattg atcatagatg gtgatattga tattgacttg
      721 aaaaccaatg agttaccttc aggagtttac ctggtaacgg tgaatggagc tgaggcgcaa
      781 ctttcacaac aattggtaat aagcaggtaa gacttcgaac acaggcaacg aacatactgt
      841 cccatgcgtc atatagatgc gtgggacagg tcttttatgc gacttgtacc ttaactttgc
      901 agctatatcc aggtattcat tcatatttaa taattgtatg cacacatgta atttccccct
      961 ccgaaaactc ggcacccttg tgttcggaat tttcttggcc actagtgtaa ttggtcagga
     1021 cacactgttt caggaatcat ttgattaccc cgctttgttt ccacctatgg gttggacaaa
     1081 tgtgaaggtt gccggtacag gcgctcccgg aacttggaag cgagttacca ccggtggttc
     1141 gccaatccag gagccacatt ctgatcccgc tgaagcagaa tttaattcgt atttctatcc
     1201 atcaggtaca gccagcgacc ttgctacacc ggtgatagat tttacagtgt caggtactaa
     1261 cctcgtttct ttttggatgt atcgcgatgc aggtgttggc accgacaaaa tggaggtgta
     1321 tgtaaacact actcaggcat cagcaggcgg caccctgata ggaacagtaa atcgaaatta
     1381 tcttgctaca ccggttgaac cggcagcggg ttggtatcaa tatactttta ccgttccatc
     1441 aacatataat acggctacaa actatgtgat ttttaaaggt gtaagtggtt atggttacaa
     1501 tatttttatt gatgacatcg ccattcttcg cgatcctgtt gttaccattc ctggctgcgc
     1561 tacctccatt ttcccggcga atggttcgat agatatttgt cctaacttga cagtttactg
     1621 ggatgtagtg ccatacgcca ccggctacaa aataacaatg ggtaataacc cacctaatta
     1681 taataacgtt gccagcaatc tggatttggg aaatgccttg agttttaccg ctatgttggc
     1741 aacaggctcc aattataaat ggaaagttaa gccttataat gtaaccggaa ttgcaggcgg
     1801 ctgtatcaat aatagtttta caacagcaac tacaccatgt tattgcgaag cggatttcat
     1861 tgaacctagt tgttcttcac tcgactttat cgatgatgtt tatacaacag gtgcagtaac
     1921 caatttcagc aatatgaata ccggttgttc accgaatccg ggtaattata cttattattc
     1981 aggaacaact gtttcggcaa accagggttc aacatttacc atgaacctcc aatcgggtcc
     2041 tgattatccg gaaggatttg ctgtttggat cgactggaac agagatggtg attttaatga
     2101 tgcagatgaa tatgcactta gttctgcacc aacaacttca ttagttagca atgtcataaa
     2161 cgttcctgcc accgcacctt tgggaacaac ccgcatgcgc atcagagcct tttataacct
     2221 taccccaccg gcaaatatgt attgcgattt gtggaatgaa ggtgaaacgg aagactataa
     2281 tattaatgtc acaacctgca catttgtgac atactatcag gatgcagatg ccgatggtta
     2341 tggcaatgcc gctgttacta caacaagctg cacaggcgca cctgctggct atactgcaga
     2401 caatactgat tgtaatgatg ctgctttttc agtacatccc ggcgctaccg aactttgtaa
     2461 cggcattgat gaaaactgca acgtaaccat cgatgataat gccgccgtag caataattac
     2521 gccttcaggc ccaacaacag tttgcaaagg cgcaagtctg atattaaatg ccaataccgg
     2581 tgttggttat acctatcaat ggaatagaaa cggcgcaaat cttgccggcg caacaggttc
     2641 ttcatatagt gttacaaagt caggaaatta taaagtaacc gtaaccgttc ccggtggatg
     2701 tactgctact tctaccgtta ctacctgcac cgtaaatgca aatcctactt caaccatcag
     2761 cactccggaa ggcaccaacc tttgcggctt acctgacttg gatcttgtag caaacgccgg
     2821 agcaggattt acttatatct ggtataaaaa tggtgttatc atgccgggta taaataccca
     2881 aatcaacaca gtaaatacaa ttggtgatta tagagtgaag gttgcaaatg cggcaggttg
     2941 ctctaaaaca tcggcaatta aaacggtatt tacatcttgt aaagaaggtg cctcaattgc
     3001 acagggagaa ctctatccga acccgaacaa cggccaaatg acattgtcat tcttcagccc
     3061 ttcaatcttg gaaggtcgcc tgaatgttca aatattagat cttaccggtc aggtcgtttt
     3121 caatgaaatg aaagacctgt ctaatgggtc ttatgccggc actatcaatg cgtcaaacct
     3181 tgctgccgga ctgtatctgg tgaaaatgtc ggcgggtgat acgcaaattc aacagcaggt
     3241 gattattgcc aagtaaggcg gtttgaaata attaaaggct gcctatttca aggcagcctt
     3301 ttttgtgaca tcacaaattg gacttcgtaa gatttaacaa tattttttct aaataaaatt
     3361 tggtaattga atacccgcat tctaccttgg ctattgatat tcatggaaac atgcatgtcg
     3421 gaggcgcccc atgaaagcgt gattagataa aaaaggtgat gtgctgaaaa cctgacttat
     3481 ttgtgattga gcttattgat cacttataaa tcgacaacac cttttgcata caaatcagat
     3541 ttgttgtttg tagcctgatc tttacaagga ctcctctcgt taaaaaaacc aaattttgtt
     3601 ttattacgta tgtatcgatt attatgattt tgtatacaaa tagtaaacaa ttatctgcac
     3661 ttatccgttt gcaattaaac tttgctgttt tatgcaatat attcatgcaa cgaaaacctg
     3721 tcactcattc atctattaat tctataacta ttctaacaat ttaaatttta aagtatgaaa
     3781 cgtttacaca cacttttact ggctgttgtg gttgcgtgtt cgagtactgc agtccatgcg
     3841 cagatttact cctacacaaa cgacctttcc ggaacccctt ccggcatagc agcaaatgca
     3901 acaggtacta acctttcccg aataaatggt accctcgaaa ccaccgcttg tcccgatgga
     3961 ttcaattcaa gccaatggtc aaaatcaacc ttgttgactc ccggaaaacc ggcagttgaa
     4021 ttctctgcaa ctcctgatgc gggatttcaa ttagacgtta cctcaatttc cgcggatatt
     4081 cgcatcaacc caaaaggtcc aactatttgg agaattgcct attctgccga cggcggggca
     4141 acttggatta caaatccttc agacattatt gttgaatcta ccacttgtga ttttaatacc
     4201 aatatatcat ttgatgtaga tgatttcagt acaaccaata ccgtaatggt gcgcttatat
     4261 ggacttggtg cacacagttc gttgaatggt aaagcagtaa ttcgcaatgc catgattgat
     4321 ggtagcgtgg ttgaccaaga tctcgatggc gatggttatg gtttccttac tgattgtgat
     4381 gatacaaacc ctgatattaa tcccggtgcg acagaaattt gtaacggaat ggatgacaac
     4441 tgtaacggcg atattgatga aggtctcaca ttcacaactt attatgccga tatggatggc
     4501 gatggttatg gtgatgccgg aatgtctgca acaacttgcg acggtactcc tgacggttat
     4561 gttatggatg caactgattg taatgatgct gatgcagcag taaatccagg tgctactgaa
     4621 atttgtaacg acgtagacga taactgcgat ggcaatattg atgaaggtct tgccgtagta
     4681 tggtatgccg atatggatgg cgatggttac ggcgatgcca ctatgatgtt agaagattgc
     4741 gctatgccag aaggttatgt aatggatgct acagattgta atgatgctga cgctgccgtt
     4801 aatccgggta tgactgaagt gtgtggcaat ggaattgatg ataactgcga tggcaatatc
     4861 gacgaatctg acgttgctgc atcatttgaa gtaatcggcg ataatccgac ttgcgaagga
     4921 acaggaactt atttcatgtc tactgctaca ggtatggata ttacttacca atggtataga
     4981 aatatgatgc caattgccgg cgcaaccggt gcaacttaca caccagctaa tgcaggttat
     5041 taccaattag aagtatcaaa tggcgtttgc tctgacatga cagataccgt tcacgtaatt
     5101 attaaacaag caccggaacc tgaactgtat atggagttag gtactgatgt ttgtgtaaat
     5161 ggttttgtga aaattaaagt aattcctaat ccgggaacag gttctactta tgtatggtat
     5221 tataatggcg atgtgttggc aggtgctact actgagcttg tagaaactac tgatgccggc
     5281 acatattatt gcgtagtaac aggccctaat ggttgctcag gcaccagtga agttgttacc
     5341 gttactgccg actgtcgtct tgccggcact gaaaacggaa cactcgctgt ttatccaaac
     5401 cctgcaaaag gaaactttac tatgcaatta aataatgctc ctgttaatgg tgaagcagta
     5461 gtgcatatta ctgacatgac aggccgtgtt gtttatgcag ttaatacaac gattgttgat
     5521 ggtcaaagcc aaatgaatgt atcactcgaa aataatattg cgagcggtat gtacatggtt
     5581 acggtgcaaa cacagcatca aatctggaat gcacagatca gtattgtaaa ataatctgat
     5641 tcacataaat aaaaaaggtc gctcagttgg gcgacctttt ttattagaat tattttctca
     5701 acaaattaat tatcatcaat cgttttctgc atatcgtttt cttccagtgt aaatccattt
     5761 tttatcgcaa ataaatattg cgctgtctga aatgaaagat cataaacaga ttctcttgta
     5821 gatacatcgg ttaaaatatt gttcatgcga ttgtaatcat ccatggtcat attggggtcg
     5881 aatacgattg tcatcacagt tgcgtattca ttcgtaaaac aactatgata aaattcaaat
     5941 aactcagaag cggcatcgcg aagagtgctg tcgccattat atgctggcat tgcttttata
     6001 agtgaaagcg aattattggt aactgttata agattgttgt aaaatggttc aacggtttct
     6061 cttgtagaac cttccacaag tatcgattga ttaaattcat tcaaagcaac agctatagta
     6121 gtttgaatgt ttactacata atcgttgtat gaaacagcat cggcaaatgg ttgcgctttt
     6181 gcagatatgc cggttacaag taaaaacgaa ataattacat ttctaacgtt catgtgtttt
     6241 agttatggtt aaaaaaaata cccggcattt aaccgggcaa taaatctttt ttgatttttt
     6301 atttacaaaa taataatggt ataagtctat agcttatgcc tcaaagctgg ttccacaacc
     6361 acatgttttc ttagcattcg ggttgttgaa cgaaaatccg cggttattca aaccttcaac
     6421 ataatcaatc tccataccgg ctaaatacat gccatgcgac ttttccataa ccaggcgaat
     6481 atcgtcgatt tgatatagct catcttgttc tttctgcagg tcaaaaccaa gaatgtaact
     6541 caatccactg cagccgcctc cctttacacc tactctcagt gcatattcgg cagggatttg
     6601 cttttctgat ataatattcc tgatctcacg catcgcgctt tcagtcaggg ttattggtga
     6661 ggttaatact tccatgtgat ttggtttttg atatcaatta caaagttact aaacgatgat
     6721 gagtctcttt tggtttcaaa tcagagattt cagttcactt gtgacatcat ttgaatcggc
     6781 cacttttgac cttaatgcct aaaaatgctt acaaaatgga tgctcctgta acaggcaaaa
     6841 tggcctcttt tcaaaaataa ataaacgaaa aagtgttaaa atgatctgta aataaaatca
     6901 ttttaaataa atttaaatat gtaattctgt gacatacctc taattacata ttcctttcga
     6961 ttattgggaa aacactacct aataacgcat cttcggactc ccccaaattg tggaaacatg
     7021 attgacagca attttttcac acctaaaccc acaccagatg aggaagttta attacttatt
     7081 agcgtgtttg gcaatggtgc tcagcttcac ggcagcaaga gcgcaattgt acaactacac
     7141 aactgacctt ggaggcgcac ctgcctcggt ggcggcaaat acgtcgggta cttcattgat
     7201 ccgcgtcaac accgcccaag ccattacagg atgtcctgat ggttttaact caaatcgctt
     7261 ttcagagtca acaactttcc tggccagtcg cccggcagtt gaattttcaa tttcaccaaa
     7321 ctccggttat cagctcaaca tcacttcatt gagtgttgat gcacgcagaa acacaaaagg
     7381 gcctgttacc tggcgtcttg cttattcagt ggatggcggt gcttcatgga tcaacaacgg
     7441 ttctgattac tctgttaccg taacttcttg cggaagtgca aacacattga catgggatat
     7501 tcctgatttc tctgttccat acacagtgta tgtgcgtgta tatgcttatg gagcactcag
     7561 taacctgaat ggtatcgcta caatgaaaaa cattgtatta agtggttcta cttcttttgc
     7621 cgatgtggat gctgacggtt atacatcaaa tgttgattgc gatgacttta atgcagctgt
     7681 taacacaggc gctactgaag tttgtaacga gatagacgat aactgcgatg gcaatatcga
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacgataact gcgatggcaa
     7861 tatcgacgaa ggcgttcaat caacttttta tgcagatgct gatggcgatg gctacggcga
     7921 tgctggtgca acaacaatgg catgttctgc tcctgcagga tatgtagctg atgcaacaga
     7981 ttgtaatgat gccaatggtt caatcaatcc gggtgcttca gaagtatgta atggtataga
     8041 tgataattgc gatggtaatg ctgatgaagg ccttactttc gatacttggt atgccgatgc
     8101 cgatggcgat agctatggtg atgcaggttc ttcagtttca acatgcgacg gcgctcctgc
     8161 aggttatgta ggcgatgcta ctgattgtaa tgatgccgac ggcgctgtaa atcctggtgc
     8221 taccgaaatc tgtaacgaga tcgacgacaa ctgcgatggt aacatcgacg aaggcgttca
     8281 atcaactttc tatgcagatg ctgacagcga tggctatggc gatgcaggtt ctacaacaat
     8341 ggcatgttct gctccggcag gatatgtagc tgatgcaaca gattgtaatg atgcagatgg
     8401 cgccattaat cccggtgcta cagaagtatg taacggtata gatgataatt gcgatggcaa
     8461 taccgacgaa ggtgttttaa tcactttcta tgctgatgcc gacggcgata cttatggaga
     8521 tgctgcagtt tcaactgatg catgttcagc acctggcgga tatgttgcca acagcggtga
     8581 ttgcaacgat gcttctaata cagtgtatcc cggtgcagct gaaatctgcg ctaacggtat
     8641 agatgaagat tgtgatggcg tggttgacct tcctgctaca attgcagccg gtggtcctac
     8701 gtcaatttgt cttggttcaa gcgtaacgct cacttgtact acaggtggaa caggactcag
     8761 ctaccaatgg cagaaaaatg gtaacccaat tgcgggtgct accggtgtat cttatgtagc
     8821 tacattgcaa ggtaactacc gttgtgtggt ttcaaaacca tcatgttctg ctacttcatc
     8881 acctattacg gttactgtat tgctcaatcc ttctgctaat atcactactc ctgatggtct
     8941 tgacctttgc ggaaaagctt atgttcgcct gaaagcgaat acaggtgcag gtttgactta
     9001 tcagtggtat aaagatggca atgctatcgg cggcgctact gcagttacct attttgcaac
     9061 tgcaaccggt gcttactatg taagagtaac caatgcaaat ggctgcagca ctaactctgc
     9121 tactgtaaac gtattctcca gctgtcgtat ggctgagggc gctttagcag gtgatgtaaa
     9181 cattatgcct aacccttcaa acggtaagtt taccatcaac tttacaactg ctcaggctat
     9241 ggatgcaagt gctcaggtga gcatattgaa ccttgccggt cagcaagttg ccggcttcga
     9301 aggtcaattg attggtggtc aaatgactgc aggcgttgat atcgctccgg tggctggtgc
     9361 ctatgtagta gttatcaatg ttaacggtgt tattttcact gaaaaactta tgatcgcgga
     9421 ataagcggtt cacaggttaa ccatattaag ggtcgctctt ccgggcgacc cttttttatt
     9481 gctcctgctg gttcaggata cgagcaagga gccgatatgc gtttaggaga gggtaaaaga
     9541 aaaagaaaaa tatttctcac attattgcac agtgattact taattttaaa tttgcaaccc
     9601 ccgaacaaag atcatggagt tattctggca gatattaggt tacattattc cggccctggt
     9661 ggtattcgga actacgtata tggtgataag agcctttctc gacagagagc ataaaatgcg
     9721 tctgttggag ttcagacaag cccatttgcg tgaggcattg cctgttcgtc ttcaggctta
     9781 tgagcgtctt acattattca ttgagcgtat atcgctgaac aatttattgc cacgtgtgcg
     9841 caagcaggaa atgaatgtat cggaattccg cacggcactt attaataata tcaggttaga
     9901 atatgaacat aatctcagtc aacaagtata tgtttctgtt gatgcctgga atatgattcg
     9961 cgcagccaaa gaagaaacta tcagcgttat caatagaaac gcgatgtcca tgttacctga
    10021 attacccgga gttgaactac ataaaaagat tatcgccgaa ctcgctgaat cagaagagga
    10081 agctacatcg caaaaagtgt tggttcaact caaaaaagaa gtgatgcagt tatatggaaa
    10141 atgatgatga tgtcattttc tgaatctgca ttttactcct cgcccaattt gttgccctaa
    10201 agacgaaata ctgcttcggc ggtaaaacaa accactatcg tgcacaagcg taattcaata
    10261 ttgcgattat tgctgctgtt ggcaattgcc tccactttgg gagcatgtca gcattattat
    10321 actgatgcac gatacgatat gcagcaaatg cgtgtcgaca acctggtgcc tgattctgca
    10381 acggcaagta tcatcgcccc gtacaaaaat gtgctcgacg ctcaaatggg tgctgttgtt
    10441 tgcactgtag ggatagattt aaatcgagcc aaacccgaat ctttactagg caatgcaata
    10501 tgcgatatga tggtaagtga agtggcaaga attaccggtg aggcaatcaa ttttggagta
    10561 tacaattatg gtggaatcag gcaggagttt ttaaataaag ggccattaac tacaggaaaa
    10621 gtatatgaat tattgccatt cgaaaatttt ggtgctgtag ttacaatgag tggaactgaa
    10681 gttgctcaat tagttcagaa gatcattgat gaaggaggat ggcctgtgtc gggaggaata
    10741 aaaattatta ttactgataa taaagcatcg caggtattta ttaatggaaa tttactggac
    10801 ccgaatggca cttatcgcgt ggcgatgaat gattatatgg ccaatggagg tgacaacctg
    10861 agttttatta aaaaggaaaa tgttgttgta ttaggtacaa ctattcgcga tatgttttta
    10921 cattacgcag caactttgca atcagaagga aaagtgctcg aatcatcaat tgaagggagg
    10981 atacagtatg acaactagaa gagccttcat acaaaagtcg gtaaccggta cagcattgtt
    11041 tggactgggg gcactatcac ttgagtcgtt tgcctccgat catattcaaa agttaaccat
    11101 cctgcattcc aatgatgtgc atagtcgcat agatccattt cccgatgacg acaagaaata
    11161 tccgggaatg ggtggtatgg caaacaaggc ggcattgata agcaagatca gagctcagga
    11221 agacaatgta ttattactgg atgccggcga catatttcaa ggaacaccat attttaattt
    11281 ttaccatgga gaattggaga tgaaactgat gactgctatg gggtatgatg caggcactat
    11341 gggcaatcat gatttcgatg ccggtattga tggttttgcc gcgcaattgc cacatgcagg
    11401 atttccaatt ttagtggcga attatgattt tacggatact gtgttgcaag acaaggtttt
    11461 acgttatact acctttcaaa agaaggagat aaaagttggt gtattcggat tgggaattga
    11521 gctggcagga ttggtgcccg gatcacttta tgcacaaact aaatatttgg atcctgtgat
    11581 aattgcacag gagatggcaa atgtgttgag atatgatgaa aaatgcgatt atgtaatatg
    11641 tttatcgcat ttgggatata gctataagga tgataaaatt tcggacatca aattggcgca
    11701 aactaccgag ggcattgatt tgatactggg tgggcatacc cacacatttt tggaagagcc
    11761 cttaaaatac aaaaatcttg ccgaccagga agtgattgtt aatcaggtag ggtgggcagg
    11821 tgtatggtta ggtaggatcg acattttttt tgagcgtaag aagggtaaaa aatggcattc
    11881 tggaaaacct gttatggttt cgaaaaaaac aagcgaataa aaattttttt tagaaaatat
    11941 tttttcacac cttcgttacc ccgctgttaa taaaacaata aaaatttttg aaatctgaga
    12001 tgagtagtaa agatcaccag tcagtgtggg caagttgtct aaagatcatc aaagacaatg
    12061 tgaatctcca gagtttcaag acctggttcg agccgatcga gccggtgaaa ttggaagatg
    12121 atgttttgac gattcaagtg cccagccagt ttttttatga atggttagag gaacattatg
    12181 taactttatt aaggaaaaca attaaacgtg agttaggggt taatgcccgt ttggagtacc
    12241 gtattgtggt agagaacagt tcaggcaata attcaccttt caccattgat tacccgaatt
    12301 acaacacagg ggctaataaa aaccccgaaa tcgcggcacc gttagttatg ggaacaagca
    12361 ttaaaaatcc attcgttatt ccgggactca agaaagtgaa tattgagtcc ggattgaatg
    12421 caaactacaa cttcgacaat ttcatcgaag gagattgtaa tcgcttgtgc cgtagtgccg
    12481 gttacgccgt agctcagaag ccgggaggca ctgccttcaa tccgcttgtg atctatggag
    12541 caacaggatt aggaaaaagc catttagcgc aatccatagg taatgaggta aaacagaatt
    12601 tcccaaataa gacagttctt tataccaacg ccgaacgatt tacgaatcag ttcattgagt
    12661 ctttgaaaaa taactcgatc aacgacttcg tgcattttta tcaattgatc gatgtgttga
    12721 tcatagatga tattcatttc tttgccaata aagcaaaaac acaggatatc ttcttccata
    12781 tcttcaacca tttgcatcag gagagtaaac agatcgtatt gacgagcgac aggcctccac
    12841 gtgatctgga aggtgttgag gagcgattat tatcgcgttt caaatgggga ctcagcgccg
    12901 atcttcaggc tccggacttc gaaaccagag tggctattct tgagaaaaag atgtatgccg
    12961 atggtatcga attacctaaa gatgtggttg aatttgttgc ctataatatc actaacaacg
    13021 ttagagagtt ggaaggagca ttggtaagtt tacttgcgca ggcttcactc aataaaaagc
    13081 aaatcgatct tgatttggct aagaagatcg taaagaactt cgtgaagaac atgtcgcgcg
    13141 aggtatctat agacttcata caaaaagctg tttgcgatta tttcgaggtg cctgtagaga
    13201 aactgaagga aaaaacacgc aaacgccagg ttgtgcaggc gcgtcagcta agtatgtatc
    13261 tgtcgaaaaa cttcaccaag cattctttga aagctatcgg aaagcatttt ggcggccgag
    13321 atcactctac ggttatccat agctgtcagg ccatccagaa tttgatggat actgacacca
    13381 aattcagaga aagtgtagat gatttgcaga aaaagatcca aatgagcatc tcttaatcgg
    13441 agaatgccac acgataaaag cctccttcca aaagattgag gcttttttat tgcaggaaaa
    13501 tgtttggaac ttgttaaaaa ggtattcctt tgttaccttt gccctcccaa tgtggtgctt
    13561 agacctaggt ggcgcatggg attgttctta aaaaatagtt tttggtgagg tgggtgagtg
    13621 gcttaaacca gtggtttgct aaaccgccgt acgggttaaa ccgtaccgag agttcgaatc
    13681 tctccttcac cgcatttaat atactctaac acgttcgggg cgtagttcag cccggttaga
    13741 atgcctggtt tgggaccagg aggccgctgg ttcgaatcca gccgccccga cataaaaaag
    13801 ccgtcaagta attgatggct ttttttattc atagcgatcg gactcatcgt ttaactcaaa
    13861 gtctttcaca tcactcatga ttcaccaatt attagtatct agagccaagt atctagtatc
    13921 tagacttggt atcaggtatc aagtatcagg tatcaggtat caagtatcag gtatcaggta
    13981 t
//
LOCUS       DAOQOU010000080        13917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2262_length_13917_cov_5.496970, whole genome shotgun sequence.
ACCESSION   DAOQOU010000080 DAOQOU010000000
VERSION     DAOQOU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2262_length_13917_cov_5.496970"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLM90_08265"
     CDS             complement(<1..514)
                     /locus_tag="PLM90_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ89405.1"
                     /translation="MIPLLFAMLLTAPDEEKLECDWIITNARIYTVDSAFSMVQSMAI
                     DDGYIVATGSNDSVMAHYWSLNVTDMAGKVIYPGFIDGHCHFLHYGVDLSYTNLVGTK
                     SFLDVVELLKKHDITKTSGWIIGRGWDQNDWDYKEFPTREALDKEFPATPVYIERVDG
                     HVALLNGRALE"
     gene            complement(511..1230)
                     /locus_tag="PLM90_08270"
     CDS             complement(511..1230)
                     /locus_tag="PLM90_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ89406.1"
                     /translation="MSHVWFITGTSSGLGKAMAEQVLMHDDTRVYGIGRRHTIVHENY
                     LPINIDLSDLAVLKQFVFPSLEAQTIVLFNNAGRLGEIAYCGNLDNEDIIQSYTVNTI
                     APHLFCNQFIAQYQAADAKKIIINITSGAAKTVYDGWGVYGAGKAALDMFTQSIAAEQ
                     QKINATNKILAYAIAPGVMQTAMQDQIRNTEAVNFSQQQKFKDLYQNNKLYHPEDVAR
                     WFWHFVQSCDSQQETVQRINL"
     gene            complement(1233..2315)
                     /gene="aroC"
                     /locus_tag="PLM90_08275"
     CDS             complement(1233..2315)
                     /gene="aroC"
                     /locus_tag="PLM90_08275"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMZ89407.1"
                     /translation="MAGNSFGTLFRITTFGESHGVALGVVIDGCPPGITLDLDFMRNE
                     MQRRKPGQSKLVTQRKEEDEFEILSGVFEGVTTGTPIGMIIRNSDQRSKDYSHLENAY
                     RPSHADYTYDAKYGFRDHRGGGRSSARETAARVLAGSVAKQILLQQHVIIQAYVDAVG
                     DLKLEKPYTELDLSLTETNMVRCPDPDMAEKMINLIAEVKKDGDTIGGTISCVITGTQ
                     PGLGDPVFDKLHADLGKAMLSINAAKAFEYGSGFSGTSMRGSAHNDLFAIDPETKKVI
                     TTTNHSGGIQGGISNGMDIYFRVGFKPVATILKPQQSINVEGETVQVEGKGRHDACVL
                     PRAVPIVEAMAALVMVDHMLIQKSMA"
     gene            2379..3599
                     /gene="hutI"
                     /locus_tag="PLM90_08280"
     CDS             2379..3599
                     /gene="hutI"
                     /locus_tag="PLM90_08280"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HMZ89408.1"
                     /translation="MASLLFLHIDRLLMADNTLTAVRKGSEMREVPSLDNAWLLLENG
                     VVKGFGGMESAPGHTDEVVDAKGRFVLPAFVDSHTHLVFATGREDEFAMRIAGKTYEE
                     IAAASGGILNSARKLREMPEEALFDAALARLEKLRNQGTGAIEIKSGYGLTTASELKM
                     LRVIRKLKEQSAMPIKSTFLGAHALPDQYKNDKDGYISLIIDEMLPKIATEGLADYID
                     VFCEQGYFTNADTDRILEAGWKFGLKPKIHVNQFTNSGGVQTGIRHKALTVDHLEVMG
                     QAEIDALKASETLPVALPGCSFFINIPYTPARSIIDAGLPLVLASDFNPGSSPSGNMA
                     FVMALACTQMKMTPEEALTAATINGAAAMELEHSTGSISIGKKPGIILTKPMRSLAEI
                     PYYFGDDLVERVFY"
     gene            3814..3939
                     /locus_tag="PLM90_08285"
     CDS             3814..3939
                     /locus_tag="PLM90_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89409.1"
                     /translation="MTKLSNPFNYDPFGLITVGRGWEVGSGYRYGFNGQEQDDEV"
     gene            4400..8266
                     /locus_tag="PLM90_08290"
     CDS             4400..8266
                     /locus_tag="PLM90_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HMZ89410.1"
                     /translation="MKSQLLFLRSILLLGIVFFSTNVKSQLHLDEEWVHTSGLPDTVD
                     YSTGKVDGSGNIYVTTNTISATEKANILTTKYNSSGVVQWSVEKDNADENDYGSAIDI
                     DGSGNVYVGAATWVNGTNYYDYMVIKYNSSGTQQWAVTYNGPGSFYDIPTDIFVDGSG
                     NVYVTGSSYGSGTLADYCTIKYNSSGSALWTSRYDYSSDQEVAAMIKNGPSSSVVVIG
                     ASENSPGSWDFAAVKYSQSTGSVVATNRNSASGSGFDQVFGADVDASGNFYITGRAAV
                     VDEGFNMRTVKIDPSMDLVWAKNYDHAGLDDESHGVVVDLDDNVYVTGWITNEDGTRS
                     FETIKYNSSGTPQWHQEVNAENVGLDAYSLKISSAVDGNIIVAGNVDNGASLDYLTVI
                     YNEDGDQLWMEQYDSPDGDDDKVNFVKADIGNAVYIGGKSYSISTATNRLVKYNWEDY
                     IVPPDDDLDHPSQFTFFENRGQIIDTDENPVNDIKYYTFHQTPGLFVFDDKISYVWGR
                     IDTTTNTDDTLSRIDLSFPDCRVGAEVHHASSQGGEYLNYFLPQCPDGITNVRNSDRL
                     LVSELYDDVDLEYYFDNQGLKYYLIIKPGYSEQNDPISLFYDGVDEVNILLDGTLEIK
                     NEVGVLIQNIADAYQIDVDGDRIDLLWDADFISIDDFEVGFDLGVYDDGLPLVIEMSL
                     TPPGGIADEIDFDNVYWSTVYGSGSGTIQITDVTYGTYHLPIIIGVNYPLWFPIGTDV
                     TTFGDLFAGGEAYISRFGPLRASNFGVIIGGSGADKPTAIDINNITLDMYISGFTTSN
                     DFYTKDFGGSSYFQDTYTNYIEEGFICQLNATGNLVRWSTYVEDVVELNSIDFKDDFT
                     EFTVVGQMEGAATASLEELDGAFYEDEEETALSYIATFNNTNHLIWGTKYWGISAQVG
                     QYDINGRLNIGGQIWGNPYWEDVGDGLGGNGEVFSSPTGGWGENGIYFTTFETDKTVS
                     WATQYGGNGTDFVYSLTSDENGNLYFVGSTSKDSYADDMEFLTYSEYEADPDDYFQET
                     YTYLEPVFSFDGYLVKFDDERNRLLSTLFGGPTHEEIYDIEVVNGDIWFVGFTQNEST
                     DFPYTFYDADCFDQSDLMAEKNGFLCIMTDEAVIKYLTYVGWIINSSTYDIAYDNTID
                     KILTTGAASYNEGDELGFAPVWKPNDFAYYSSLNPAGIAKNSSSFITEWLRLPGDVVI
                     HSDELYKYEIDVYPNPCSSLVNIEAVNLTGEKFVQVFDIRNKKVFEKIYYEDQIDINV
                     GNLAPGFYLGKIQQENSTIDFKFIKL"
     gene            8263..9510
                     /locus_tag="PLM90_08295"
     CDS             8263..9510
                     /locus_tag="PLM90_08295"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ89411.1"
                     /translation="MRYIITFFLFFFFLNHGFCQWTLIYEFPEFSDIKDIEINESNEL
                     FIAVDESIYKSSSEGISWDLKYSGSYEIRSIKFFGTDTGYAAKAYGDGTGICLRTVNN
                     GEFWEIPLVFTSYWPDNNDLVLLNGHSIMLAGGQAVGYAQISDDSFETCDVKYPDPTT
                     KSFSAIDCIGGDTCIIIAGDPYGFDDEVGGIFKTEDHGQNWNEKEIVNLGRDIVFTSK
                     EVVYVLGVFDLLKSIDGGNTWNVVKSYNIDKFGEFCAIKFINDSVGYLGEKFNGLDSI
                     AIYFTSNGGLSWSATEIPDLPISLSAIDCIDEKNCYFTTNAGEIYRTINGGMTSISVI
                     DDYIDEVLILPNPINNIVTIYLNNAGLFNPILTNVFGQNCKFEIISRSPDLIKCDISN
                     LTRGIYFISLDDNLNTTFKFIKL"
     gene            9510..9779
                     /locus_tag="PLM90_08300"
     CDS             9510..9779
                     /locus_tag="PLM90_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89412.1"
                     /translation="MGDSFALIVLNLLLLSTISKGQWVKIAEVPHSSLDIQFLDINTG
                     FIIGDQFVFKTADGGYTGDSTELGNSLDLLLEFDFIDADYRIYCR"
     gene            9733..10305
                     /locus_tag="PLM90_08305"
     CDS             9733..10305
                     /locus_tag="PLM90_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89413.1"
                     /translation="MNSILSMRITGYIVGDVNGYQGFYTMDQGGFWLPRTPYILYGQV
                     AMINGNEVVVSIYPPEPAIYHLYFEEALVETLLPGVGGHDIAVFPHDTIYVGGGGFAK
                     SFDKGENWTVTEMPGILHMDFPSSQVGYSTFDDLLFKTTDFGDSWTELELPDYFDACT
                     ALEYANDSVGYYPCTKSASYDGIMKTNDSV"
     gene            10520..11698
                     /locus_tag="PLM90_08310"
     CDS             10520..11698
                     /locus_tag="PLM90_08310"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMZ89414.1"
                     /translation="MKEVYIVSIARTPIGSFGGALASVTAPQLGATVIKAAVERAGIK
                     GSDVQEIFMGNVIAANIGQAPVTQAMLAAGLPDTTASTTINKVCASGMKAIMIGAQSI
                     MLGTNDIVVAGGMESMSNVPYYLTKERWGAKLGHSEVVDGVIKDGLWDPYGNKHMGSC
                     GETCAREYKFSREDQDNYAIESYKRAAEAWKTGAFKHEIVPVVIPQRKGDPIVVSEDE
                     DYTKVKFEKVPTLAPAFERDGTITAANASNLNDGASAVVLMSKERAEAMGIKPLAKIL
                     AFADAQQAPEWFTTTPAKAMPKAIAAAGLQISDIDYFEINEAFSVVALANMKELNISH
                     QKTNIYGGGVSIGHPIGSSGARITITLLSALMNNNARYGCAGICNGGGGASAIVIERI
                     "
     gene            11809..12723
                     /locus_tag="PLM90_08315"
     CDS             11809..12723
                     /locus_tag="PLM90_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89415.1"
                     /translation="MRKSIVAFLLLLISTASYAQKSDKEITPEDMTKLLAYQDTLKWI
                     GDSVVQSPHWEMREQSCIIFLKMLKTALKTPNSYQFAFDSVPTMSVVNAPDNTFRILT
                     WQMQMKDHSHRYYGAIQRNSSALDITPLIDMSMFIGNPEDTLLSANSWYGCLYYNITQ
                     KKYKGKTYYFLFGWDGNDMWSNKKMVDVLTFNEMGVPMFGYPMFLAKDDINPQTRIII
                     EYKEDASPVLNYDEQLKMIVISYLRPENPMSEGIYFTYIPDGTYVGFYFKRGLWRYKD
                     QVFDRTMDAPPDYTPKREGENPNIYKNE"
     gene            complement(12739..13440)
                     /locus_tag="PLM90_08320"
     CDS             complement(12739..13440)
                     /locus_tag="PLM90_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ89416.1"
                     /translation="MKLKKYRQNYEDFVIEGEKLVTEALLENVYPDLLIATADWLQAN
                     RRSIPEAVQVVETSMADIEKISSLSTPQPVLALMKKKEVSINTIDITQKWSLALDGIS
                     DPGNFGTILRTADWFGIDTVLCSNDCVDLYNPKVIQSTMGSIFRMSVCYFDLHDYLAS
                     CNLPKYAAVLDGEMLDNHIWPEKGVLVIGSESHGISSKVLATCNHAITIPSYGKSESL
                     NAAIATGILLHDIKR"
     gene            13450..>13917
                     /locus_tag="PLM90_08325"
     CDS             13450..>13917
                     /locus_tag="PLM90_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89417.1"
                     /translation="MFYLRLRQHSVNRVTLVCLIAVIAVFVAGCNSTKYLQNDEFLYQ
                     GAELVFTDTSFTGVDTNALRSDLLDLSRQDPNEKVLGLFPIKTWLYTLGDTAIDHYIA
                     YQEQYDTRFLFVFDYDTLLKFITPLSDPKSNFREWLTTKAGEAPVLIDSGIGDE"
BASE COUNT         4084 a         2595 c         3026 g         4212 t
ORIGIN      
        1 gttccaatgc acgaccattt aataacgcaa catgaccatc aacacgctcg atataaacag
       61 gagttgcggg aaattcttta tccagcgctt ctcgcgttgg aaattctttg taatcccaat
      121 cattttgatc ccatcccctt cctattatcc atccgcttgt tttggtaata tcgtgcttct
      181 tcaataattc aacgacatcg agaaatgatt ttgtgcctac gagattggta taggataaat
      241 cgactccata atgcagaaaa tgacaatggc cgtcgataaa tccgggataa ataaccttac
      301 ctgccatatc cgttacatta agcgaccaat aatgcgccat tacagaatcg ttagatcctg
      361 tggctactat atatccgtcg tcgatagcca tagattgcac catggaaaat gcactatcta
      421 ccgtataaat cctggcattg gtaatgatcc agtcgcactc gagtttttct tcatcgggtg
      481 cggtaagtaa catggcgaat aataatggta tcataggttg atgcgttgaa ctgtttcctg
      541 ctgactatcg catgattgca caaaatgcca aaaccatcgc gcaacgtctt cagggtgata
      601 caatttattg ttttgataaa ggtctttgaa tttttgttgt tgcgaaaaat tcacagcctc
      661 cgtatttcta atttgatcct gcattgccgt ttgcatcact ccgggtgcaa tggcataagc
      721 aagaatttta tttgttgcat ttattttttg ttgctcggcg gcaatacttt gcgtaaacat
      781 atccaatgct gcctttcccg ctccataaac gccccatcca tcgtataccg tttttgcagc
      841 gccactggta atattgataa ttattttttt ggcatcagct gcctgatatt gcgcaataaa
      901 ttgattacaa aataaatgtg gggcgatggt atttactgta taggattgaa taatatcctc
      961 attatccaaa tttccgcaat aagctatttc acccaatcga cctgcattat taaacaatac
     1021 aatagtttga gcttccaggc ttggaaatac aaactgcttt aaaactgcaa ggtcacttaa
     1081 atcgatattg attggcaaat aattttcatg aacgatagta tgccttctgc caattccata
     1141 aacgcgcgta tcgtcatgca tcaatacttg ttcggccatt gcttttccaa gtccggatga
     1201 agtacctgtt atgaaccaaa catgcgacat ggttatgcca ttgatttttg aataagcata
     1261 tgatcaacca tcaccaatgc agccatcgcc tctacaattg gcactgcgcg aggtaacaca
     1321 catgcatcat ggcgaccttt accttcaacc tgcactgttt ctccttcaac attaatgctt
     1381 tgctgaggtt ttaaaattgt agctacaggt ttaaatccta ctctgaaata aatgtccatt
     1441 ccattgctga tgccaccttg aataccacct gaatgattgg tagttgtaat tacctttttt
     1501 gtttcggggt caatagcaaa taaatcgtta tgagcactgc cgcgcatgct tgttccggaa
     1561 aatccgcttc cgtattcaaa tgctttcgcg gcattaatgc tcagcattgc ttttcctaaa
     1621 tctgcatgca gtttatcaaa aacagggtca ccgagtcctg gctgagttcc ggtaatgaca
     1681 caagaaatag ttccgccaat ggtatcgcca tccttcttca cctccgctat gagattgatc
     1741 attttttctg ccatgtccgg atcaggacaa cgcaccatat ttgtttctgt tagagacaga
     1801 tccagttcag tgtatggttt ctccagtttg aggtcgccca cagcatcaac atacgcttga
     1861 atgatcacat gctgttgtaa cagaatttgt tttgcaacgc ttcctgccaa tacacgcgcg
     1921 gcagtttctc ttgcactgct gcgtccgcct ccgcgatgat cgcgaaaacc atatttggca
     1981 tcataggtat aatcggcatg actggggcga taggcgtttt ccaggtgact ataatcttta
     2041 gaccgctggt cgctgtttct gatgatcata ccaattggcg tacctgtagt gacaccctca
     2101 aatacaccgg ataatatttc aaattcatct tcctccttgc gttgcgtaac cagtttcgat
     2161 tgtccgggtt tgcgacgctg catttcgttg cgcataaaat cgaggtcgag ggtgataccg
     2221 ggtggacagc cgtctatcac aacaccaagc gctacgccat gcgattcgcc aaacgtggtg
     2281 atgcggaata gcgttccaaa agagttccct gccatagggg cgaaattacg ctatcgtagc
     2341 aattttttgc attcaacaag ttcctgcatc tttgtcccat ggcgtccctg ttgtttttgc
     2401 atatcgaccg gctccttatg gccgacaata ccctcactgc agtgcgcaaa ggaagcgaaa
     2461 tgcgcgaggt tccgtccttg gataatgcct ggttgttgtt ggaaaacggc gtggtgaaag
     2521 ggtttggcgg catggaatca gcacccggac ataccgacga agtggtagat gccaaggggc
     2581 gatttgtgtt acccgctttt gtcgactccc atacccatct ggtatttgcc accggtcgcg
     2641 aggatgaatt tgccatgcgc attgccggca aaacctacga agagattgct gcagccagcg
     2701 gaggcatcct caacagcgcc agaaaactcc gcgaaatgcc cgaagaagcc cttttcgatg
     2761 cagcccttgc ccgcctcgaa aaacttcgca accaaggcac cggcgctatc gaaataaaga
     2821 gcggctacgg ccttacaaca gcatctgagc tcaagatgct ccgtgtcatc agaaaattga
     2881 aggagcagtc tgctatgccc ataaaaagca cctttcttgg ggcacatgca ctccctgacc
     2941 agtataaaaa cgataaagat ggctacatca gcctcataat cgacgaaatg ctgcccaaaa
     3001 tagccacaga gggcttagcc gattatatcg atgttttctg tgagcagggc tacttcacca
     3061 atgcagatac agaccgcatt ttggaggcag gttggaaatt tggattgaag ccaaagatcc
     3121 atgttaacca gttcaccaac agcggtggcg tgcaaacagg catcagacac aaagcattga
     3181 ccgttgatca ccttgaagta atgggacaag ccgaaataga cgccctcaaa gcttcagaaa
     3241 ccttgcctgt agcacttccg ggctgttcat ttttcataaa cataccctat acccctgcca
     3301 gaagcatcat tgacgcagga ttgccacttg ttctcgcatc agatttcaat cccggttctt
     3361 ccccatcggg caatatggca tttgtaatgg cactagcctg cacgcaaatg aaaatgaccc
     3421 cggaagaagc gcttacagcc gccaccatca acggagcagc agccatggaa ctcgaacaca
     3481 gcactggaag catctccatc ggcaaaaaac ccggcatcat ccttacgaag ccgatgcgct
     3541 cgctggcgga gattccgtat tattttgggg atgatttggt ggagagggtg ttttattgat
     3601 atgcagattc cactgaggtg aaatgtcatt gcactccatg tgctgataaa ttcatacttg
     3661 caacgagtta cctattacca aaaacggatg tgtacatgaa aacaacgata caaacgaagc
     3721 acaaataaat ccgtaggact acatcttcaa atcttcacgt tttttgcatt ttaagctttt
     3781 aatcgttact tttaacaaac accatcccca tctatgacaa agctttcaaa tccatttaat
     3841 tatgatccgt ttgggttgat aactgtgggt aggggttggg aggttggaag tggctacaga
     3901 tacggtttta atgggcaaga gcaggatgat gaagtttaag gaaatagcaa tctaaacacc
     3961 gcagagtatc gggggcatga tactcgtctt ggtaaaaggt ggaatgtgga tcctgtaaat
     4021 aaatcagatt tatcatgtta caatgttttt agcaatagcc ctatagtaag tactgatgtg
     4081 aagggcgata atgatgattg gtttcaagat gattcaaatg gtagcatcag atggattgat
     4141 agtaacatgg atacatatgt tgatttggat ggtaaatcat tttctaacat tggcacatca
     4201 ataacaattg aaaccaaaag tgatattaag actccgtctg acatccagag gactcttcat
     4261 aacaagtgtc cagtttttag gattcaccat ttatggtgaa ttttgaggaa attgctaaaa
     4321 ttttacacaa aaaatttggg caaccaaata ttgaaggtta aatttcaata atatttcttt
     4381 taccctaaaa ccaattttta tgaaatctca attacttttc ttgagaagta ttctcctctt
     4441 agggattgtt ttcttttcaa ctaatgtaaa aagtcaatta caccttgatg aagaatgggt
     4501 gcatacgtct ggtttaccgg ataccgtaga ttatagtaca ggaaaagttg atggttctgg
     4561 aaatatttat gttaccacaa atactatttc tgcaactgaa aaagcgaaca ttttaacaac
     4621 aaaatataat tcctctggtg ttgtgcaatg gtcagtggaa aaagataatg cagatgaaaa
     4681 tgattacggc tcggcaattg acattgatgg ttcaggaaat gtctacgttg gtgccgcaac
     4741 ctgggtgaac ggtactaatt attatgacta catggttatt aaatataact cttcgggtac
     4801 gcaacaatgg gcagttacct acaatgggcc cggaagcttt tatgatattc caaccgacat
     4861 ttttgttgat ggatcaggaa acgtttatgt aactggtagc agctatggaa gtggaactct
     4921 agcggattat tgcactatta aatataattc ctccggctcg gcactatgga cttcacgata
     4981 tgattattca tctgatcaag aagttgcagc aatgattaaa aatggccctt caagtagcgt
     5041 cgttgttatt ggagcaagcg aaaattcacc aggaagttgg gattttgcag ctgtaaaata
     5101 tagtcagagc actggatcag ttgtagctac gaatcgtaat tcagcatctg gtagtggctt
     5161 tgatcaggta ttcggagccg atgtcgacgc ttcaggaaat ttttatataa caggacgtgc
     5221 tgctgtcgta gatgaaggtt ttaatatgcg aacagttaaa attgatccat ctatggattt
     5281 agtctgggca aaaaattatg atcacgcagg actagacgat gaatctcatg gagttgtggt
     5341 tgatctagat gataatgttt acgtaacagg atggattaca aatgaggatg gaacaaggtc
     5401 atttgaaaca attaaatata atagcagtgg caccccacag tggcatcagg aggtgaatgc
     5461 agaaaatgtt ggattagatg catatagtct aaagataagt agtgctgttg atggaaatat
     5521 tattgttgcg ggtaatgttg acaatggggc gagcttagat tatttaacgg ttatttataa
     5581 cgaagatggt gatcagctgt ggatggaaca gtatgacagt ccagatgggg atgatgataa
     5641 agtgaatttt gtaaaagctg atattggaaa cgctgtttac atagggggta aatcatattc
     5701 tatttctact gcaacaaata gattggttaa atataattgg gaagattata tagtgcctcc
     5761 agacgacgac ttagatcatc cttcgcaatt tacctttttt gaaaatcgcg gtcagattat
     5821 tgacactgat gaaaatcctg ttaacgatat aaaatattat actttccatc aaacccctgg
     5881 cttgtttgtg tttgatgata aaataagtta tgtatgggga agaattgata caacaacaaa
     5941 tactgatgat actttatcga gaattgatct tagttttccg gattgtagag taggcgctga
     6001 ggttcaccat gcatcaagtc agggtggtga atatttaaat tattttttac ctcaatgccc
     6061 tgatggaatt acaaatgtga gaaatagtga taggctttta gtatctgaat tgtatgatga
     6121 tgtggatctg gaatactatt ttgataacca aggtcttaaa tattatctta ttattaagcc
     6181 aggttatagc gagcagaatg atcccatttc gttattttat gatggagtcg atgaagtaaa
     6241 tatactttta gatggtacac ttgaaattaa aaatgaagta ggtgtattaa ttcagaatat
     6301 cgctgatgca taccagatag atgttgatgg agatagaatt gatttattat gggatgcgga
     6361 ttttatttca attgacgatt ttgaagttgg ctttgactta ggggtatatg atgacggtct
     6421 tccgttagtc attgaaatgt cgttaactcc tccgggaggt atagcagatg aaattgattt
     6481 tgacaacgtt tattggagta cggtttatgg atcagggtca ggaactattc agattacaga
     6541 cgtcacttat ggtacatatc atttaccaat tattataggt gtcaattatc cattatggtt
     6601 tcctatcgga actgatgtta caacttttgg tgacttattt gcaggaggag aagcatatat
     6661 aagtcgattt ggcccattga gggcaagtaa ttttggggta attattggtg gttcaggagc
     6721 ggataaaccg actgctattg atataaataa cataacatta gatatgtata tatctggatt
     6781 tactacaagc aatgattttt atacaaaaga ttttggaggt tcttcttatt ttcaagatac
     6841 atatacaaat tatatagagg aaggatttat ttgccaacta aatgctacag gaaatttggt
     6901 aaggtggtct acctatgttg aggatgttgt agagttaaat agtatagatt tcaaagatga
     6961 ttttacggaa ttcacagttg taggacaaat ggaaggcgct gcaacagcat cgttagaaga
     7021 attggatggg gctttttatg aagatgaaga ggaaacagcc ttgagttata tagctacatt
     7081 taacaacaca aatcatctta tttggggaac aaaatattgg ggaatttcag ctcaggtagg
     7141 tcagtatgat attaatggta ggttaaatat cggaggtcaa atatggggca atccttattg
     7201 ggaagacgtt ggtgatggat taggcggaaa cggagaagtt ttttcatctc ctaccggcgg
     7261 ctggggtgaa aatggaattt attttacgac ttttgagaca gataagactg tatcatgggc
     7321 tactcagtat gggggcaacg gcactgactt tgtttatagc ttaacctctg atgaaaatgg
     7381 gaatttatat tttgtagggt caacatctaa agactcttat gcggatgata tggaattttt
     7441 aacatattca gaatatgaag cagatcctga cgactatttt caagaaacct atacctattt
     7501 agaaccggtt ttttcctttg acggatatct tgttaagttc gatgatgaaa gaaatcggct
     7561 gttatctact ttgtttggag gaccaacaca tgaggaaatt tatgatattg aggtggtcaa
     7621 tggggatatt tggtttgttg gattcacaca aaatgaaagt acagattttc catatacctt
     7681 ttatgatgcc gattgttttg atcaaagtga tttaatggca gaaaaaaatg ggtttctctg
     7741 tataatgact gatgaagctg tcattaaata tttaacatat gttggatgga taattaattc
     7801 ttcaacctat gatatagcat atgataacac cattgataaa atcttaacaa ctggtgctgc
     7861 ttcctataat gaaggggatg aacttggctt tgccccggtc tggaagccaa atgatttcgc
     7921 atattattcg tcattaaatc cagcaggaat tgcaaaaaat tcaagctctt tcataaccga
     7981 atggctgaga ttaccaggtg atgtggtgat tcattctgat gaactctata agtatgaaat
     8041 agatgtatac ccaaatcctt gttcctcttt agtcaatata gaagctgtta atttaactgg
     8101 ggagaaattc gtacaagtgt ttgatatcag aaataagaaa gtttttgaga aaatctatta
     8161 tgaagatcag atagatataa atgtgggtaa tttggcaccc ggtttttacc ttggaaaaat
     8221 acaacaagaa aactccacga ttgactttaa gtttattaaa ttatgagata tataatcact
     8281 ttttttctat ttttcttttt tctaaatcat ggtttttgtc aatggacatt gatttatgaa
     8341 tttccagaat tttccgatat aaaggatatt gaaattaatg aatcaaatga attatttatc
     8401 gcggtcgatg aaagtattta taagagttct tctgaaggaa tttcttggga tttgaaatat
     8461 tcaggttcgt atgaaatacg aagtatcaaa ttttttggta ctgataccgg gtatgccgct
     8521 aaagcatatg gtgatggaac tggtatatgt ttaaggactg taaataatgg agaattttgg
     8581 gaaattcctt tggtatttac atcatattgg ccagataata atgatttggt tctgttgaat
     8641 gggcattcaa taatgcttgc cggggggcaa gccgtgggat atgctcaaat ttcagatgat
     8701 tcgtttgaaa cttgtgatgt aaaatatccg gatccaacca ctaaaagttt ttcagctatc
     8761 gattgcatcg gaggtgacac gtgtataata attgcaggag acccatatgg ttttgatgat
     8821 gaggtcggag ggatttttaa aacagaagat catggccaaa attggaatga aaaagaaata
     8881 gttaatcttg ggagagacat agtttttact tcgaaagagg tggtatatgt tttgggtgtt
     8941 tttgatttat taaaatcaat tgacggtgga aatacgtgga atgttgtaaa atcgtataac
     9001 attgacaaat ttggtgaatt ctgtgcaata aaatttatta atgacagtgt gggctatctg
     9061 ggtgaaaaat ttaatgggct agatagtata gcaatttatt ttacttcaaa tggaggtctt
     9121 tcatggagtg caaccgaaat cccggattta cctatttctc tatcagcaat tgattgcatt
     9181 gatgagaaaa attgttattt tacaactaac gcgggtgaaa tttatagaac tataaatggt
     9241 ggaatgacct ctatttccgt aattgatgac tatattgatg aagtattaat tttaccaaat
     9301 cccattaata atattgttac tatttattta aacaatgctg gattatttaa tcccatactt
     9361 actaatgtat ttggtcaaaa ttgcaaattt gaaattattt cccgtagtcc tgatttaata
     9421 aaatgtgata tttcgaattt aaccagagga atatatttta tatcacttga cgataattta
     9481 aatacaacgt tcaaatttat taaattataa tgggtgattc atttgcgttg atagttttaa
     9541 atctattgct cttatcgact atttcaaaag ggcaatgggt aaaaattgct gaagtacctc
     9601 acagctcatt agatattcag tttttagata ttaatactgg ttttattatt ggggatcaat
     9661 ttgtatttaa aactgctgat ggtggttata ccggggattc aactgaacta ggaaactcac
     9721 tagatctttt gcttgaattc gattttatcg atgcggatta caggatatat tgtaggtgac
     9781 gtaaacggtt atcaagggtt ttatacgatg gatcaaggag gattttggtt gccaagaacc
     9841 ccgtatattt tgtatgggca agttgctatg ataaatggca acgaggtagt tgtgtcaata
     9901 taccctcccg aacctgcgat ctatcatctt tattttgaag aggctttggt agaaacgtta
     9961 ttacctggtg tgggtggtca cgatatagct gtttttccgc acgatactat ttatgtcggc
    10021 ggtggtggat ttgccaagag ttttgacaaa ggtgaaaatt ggactgtcac tgaaatgccg
    10081 ggaattttac acatggattt tccttcttcg caagttggct attccacctt tgatgacttg
    10141 ctatttaaaa caactgactt tggtgattca tggactgaat tagagttgcc agattatttc
    10201 gatgcttgca ctgcgcttga atatgccaac gattctgtag gctattaccc ttgcactaaa
    10261 agtgcttctt atgacggaat tatgaaaact aatgattcag tctgacaacc aacatcgcgg
    10321 acaacagctc atcaccattg tgaaagccac gtggggtgtt ttgctggtca ttgcattcct
    10381 gcttcttgcc cagtgaactt ttccggccac tttttggtag ctgaaaaggt acgcaggata
    10441 tgtctcagca tgatgctagt cttttcgacc tcctcatggt aaaatccgta tctttgcggc
    10501 gaaattcaca gttaacatta tgaaagaagt atatatcgtt tcaatagccc gtacgccaat
    10561 tggtagtttc gggggagctt tggcctctgt aacggctcca caactgggtg caactgttat
    10621 caaagccgcc gtagaacgcg ccggcattaa gggtagtgat gtgcaggaga tctttatggg
    10681 taatgtaatt gcggctaata tcggtcaggc tcctgtaaca caagccatgc ttgctgcagg
    10741 tcttccggac accacagcct ctactaccat caacaaggta tgtgcttcag gaatgaaggc
    10801 cattatgatc ggtgcgcaat ctatcatgct gggaaccaat gacatcgtag ttgcgggtgg
    10861 tatggagagc atgagcaatg tgccctatta cctcaccaaa gagcgctggg gtgcgaaact
    10921 gggacatagc gaagttgttg atggtgtgat taaagacggt ctctgggatc cttatgggaa
    10981 caaacacatg ggttcatgcg gcgaaacatg tgcccgcgag tataaattct ctcgtgaaga
    11041 ccaagacaat tacgctattg agagctacaa acgtgctgca gaagcatgga aaacaggtgc
    11101 tttcaaacat gaaatcgttc cggttgtgat cccacagcga aaaggagacc cgatagtggt
    11161 ttcagaggat gaagattata ctaaagtaaa gtttgaaaag gttccaactt tggctcctgc
    11221 attcgaaaga gatggtacta tcacggcagc caatgccagc aatctcaacg atggtgcttc
    11281 agcggtggtg ttgatgagca aggaacgcgc agaagccatg ggtatcaagc ctttggcgaa
    11341 aatattggcc tttgctgatg cccaacaggc accggagtgg ttcactacta ctcccgcaaa
    11401 agcaatgcca aaagctattg cagcagcggg tttgcagatc agtgatatcg attatttcga
    11461 gatcaacgag gccttttcag ttgtagcact cgccaatatg aaggaattga acatttcaca
    11521 ccagaaaacc aatatctatg gtggtggcgt ttcaattggt caccctatcg gttcttccgg
    11581 agcacgcatc accattacct tgttgagtgc cttgatgaat aacaatgcac gctatggttg
    11641 tgccggtatc tgcaatggcg gcggtggtgc aagcgcaatc gtgattgagc gtatataatt
    11701 gcagtatagc atggattatt cacatcctgc gacacaataa atgagatgaa atttgcatta
    11761 taatgcctga gttttgcact cgggcatttt ttttgccaaa catgatctat gcgtaaatct
    11821 attgtagcct ttttactgct gcttatttca acggcatcct atgcacaaaa aagtgataag
    11881 gaaataacgc cggaggatat gacgaagcta ttggcctatc aggacacgct caaatggata
    11941 ggtgattcgg ttgtacagag tccgcattgg gaaatgcgcg agcaatcatg cattatcttc
    12001 cttaaaatgc tgaaaaccgc cctcaaaacc cccaattcat accaatttgc ctttgattct
    12061 gtacctacaa tgtcggtcgt aaatgcacct gacaatacat tcaggatatt gacctggcaa
    12121 atgcagatga aggaccatag tcatcgttat tatggtgcca tacagcgcaa ttccagtgcg
    12181 ttggatatta ctccgctcat cgacatgagt atgtttatag ggaatccgga ggatacactt
    12241 ttatcggcaa acagctggta tggctgtctg tattataaca tcacccaaaa gaaatacaag
    12301 ggcaagacct actacttttt atttggttgg gacggcaatg acatgtggag caataaaaag
    12361 atggtagatg tgcttacatt caatgaaatg ggtgttccga tgtttggata tccgatgttt
    12421 ttggcgaagg atgacatcaa cccacagacg cgcataatca tagaatacaa agaagatgcc
    12481 tcgcctgtgt tgaattacga tgagcagcta aaaatgattg tgatcagcta cttgcgaccg
    12541 gaaaacccga tgtcggaagg aatttacttc acctatattc ccgacggcac ctatgtgggc
    12601 ttttatttca aacgaggtct ttggcgttat aaggatcagg tgtttgacag aactatggat
    12661 gcacctccgg attacacgcc taagcgcgaa ggtgagaacc cgaatattta taaaaacgag
    12721 taatcagcaa agcgagattt atcgcttaat atcatgcagt aatatacctg ttgcgatagc
    12781 ggcatttagg ctttcagatt taccgtatga agggattgta atggcatgat tgcaagttgc
    12841 caatactttt gatgatatgc cgtgagattc gctcccgatg accagcactc ccttctcagg
    12901 ccaaatgtgg ttatcgagca tttcgccatc aagaacagcg gcgtattttg gaagattgca
    12961 agatgcaaga taatcgtgta gatcaaaata acataccgac atacgaaata ttgaacccat
    13021 ggtactctga atcactttgg gattatacag gtcgacgcaa tcattgctgc aaagaaccgt
    13081 atcaattcca aaccaatctg ccgttctcag tattgttccg aagtttcccg gatcactgat
    13141 gccatctaaa gcgaggctcc atttttgggt aatatcgatt gtgttaatgg atacttcttt
    13201 tttcttcatc agtgccagca caggttgagg cgtgctgagt gatgatattt tttctatgtc
    13261 cgccattgaa gtttcaacaa cctgaacggc ttcgggaata gatcttcgat tcgcctgtaa
    13321 ccaatcggca gtggctatta ataggtcagg ataaacgttt tccaacaagg cttccgttac
    13381 taacttctcg ccttcaatca cgaaatcctc ataattttgg cgatactttt ttagtttcaa
    13441 gccctggaga tgttttatct gcgccttcgt caacatagcg ttaaccgcgt cacactggtc
    13501 tgcctcatcg cggtaattgc agtgtttgtt gcgggctgca attctactaa atatctgcaa
    13561 aacgacgaat tcttgtatca gggagccgag ttggtgttta cggataccag ctttaccggt
    13621 gttgatacaa atgccctgcg atcagatctg ttggatttaa gtcgtcaaga ccccaatgaa
    13681 aaggttcttg gtctttttcc aatcaaaaca tggttgtata cgctgggtga cacggcaata
    13741 gatcattaca tcgcatacca ggaacagtat gatacgcgat tcctatttgt atttgattac
    13801 gatacacttt taaaattcat cactcccctc tccgacccta agagtaattt tcgcgaatgg
    13861 cttacaacca aggctggaga agcaccagtg ttgatagata gcggtatagg cgatgaa
//
LOCUS       DAOQOU010000081        13644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2322_length_13644_cov_6.825962, whole genome shotgun sequence.
ACCESSION   DAOQOU010000081 DAOQOU010000000
VERSION     DAOQOU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13644
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_13644_cov_6.825962"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2030
                     /locus_tag="PLM90_08330"
     CDS             <1..2030
                     /locus_tag="PLM90_08330"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ89418.1"
                     /translation="SDKANVQAVYCLANTPIPYGNPSGLSAKIKNESTSAETFTVNFT
                     VKEKVSGTVRYSSSSGVSFTAGEVKTVTATGWNPTIQETDTVIVDVPALPGETVVANN
                     SLGYRHVVNSDTYGYADLSAATGGVGFGAGAGQILNRHTLTGCSSVEAIRVYIFGAAS
                     IGNTIRGVVNDITGIQIGVSADHVVTAGEVDTWITLPILSPPFITNQDVLFGIEQTAG
                     IGAAAYFPVGTQTESSPTRDFAFYSNNPGGGALVGPYTTLNRWMIEGVVTPLSTDPTV
                     SGPTEACDGDPISFTASFTELNPAAVVNWYTDGCGTTLVGTGMTFSTTAMAGMTTYYV
                     RAEDACNGVNTACASITITVGVSEVYFADADGDTYGDPGATVLSCDGAPTGYVLDPSD
                     CDDTNSSVYPGATEVCDGLDNNCDGNIDEGVVEAVITAPGGLVACKPLSVLLQTTAIP
                     GYTYQWFKNGNIITGAINPTYSASKPAYYQVQVNTPGGCFALSDAVLVNILNAPNANI
                     SAPNGTSLCTTVKLKASYDATYTWQWRLGGVDIGGATNYLYYPSTAGSYTCVVTSAEG
                     CSRETASIAVTACREGGAISEEINTINVYPNPSAGTFTLEIMSGDEFSGKADVYVMNM
                     IGEVIYNNNIDITNGYINNTITLADGIAGGLYLVKVMANGHEYTQQVVISK"
     gene            complement(2171..3274)
                     /locus_tag="PLM90_08335"
     CDS             complement(2171..3274)
                     /locus_tag="PLM90_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ89419.1"
                     /translation="MRWWQKLILFITIIFALLLIGAMLAMYISPEKFWPLAFLGLGYF
                     PILLCYMFFMLLWLFMRRKIFYGMLIILTLGFTAHLRHFSLGGLKRSSDSEKPVYTLM
                     QYNVQGFDAYNKEGKYKNRDQIIANIANAKPDILCMEEFNTYTNHRKEKSNLDMVMTA
                     TGLKNYYYYKAFENPKTTRSFGLMIMTKFPIIDTGHLEYLSLSPLNTTIYADLLIEND
                     TVRVFCSHMQSTQLNHYDLEFIEASNEAETDFDADRITTKLKMSFSLRAQEADTVATH
                     IKASPFPMISCGDFNDTPVSYTYHKMSHGMEDAFLKRGFGVGATYAPFPFIRIDFMLF
                     KDDAFDIHDYYTIKDKSSDHYPCITKFSIPAKD"
     gene            complement(3276..4169)
                     /locus_tag="PLM90_08340"
     CDS             complement(3276..4169)
                     /locus_tag="PLM90_08340"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ89420.1"
                     /translation="MAFFDDIKYQWQHGTAAMKLIIVNVAIYLAQSLGLLVAYGFHQK
                     EAYLDFLVNWFWANSNISTLATRPWTLVSYMFMHDPQNIFHIVSNMIYLFFFGRIFSD
                     ITNQKLAYPLYFAGGFAGFFLALISFYLFPGYQENIGGYFVGASAAIMAIVIATTTLM
                     PNYTVFLYFLGPVKLKYIAAFVVIVDVISIPGESNIGGHIAHLGGALFGYLYIRSYRK
                     SGNWFSWWPHFESRVWQIFHRNKLRVAYNYQRQDTDDRRAKEIEDQKKLDAILDKIKA
                     SGYESLSRQEKDFLFRMSKEK"
     gene            complement(4187..4870)
                     /locus_tag="PLM90_08345"
     CDS             complement(4187..4870)
                     /locus_tag="PLM90_08345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ89421.1"
                     /translation="MSQIQFTRFNYLPPVIKNLLIINGLVYLATFTFPELKETLALHY
                     PSTPEFRPYQIVTHMFTHENFAHVFFNMFSLWMFGAVLENVWGPKRFLQYFLITGLGA
                     ALCFTAYHAYEVYQSVGTIFPSSSITDTPLRWNVNGLNYSAVGASGAIYGILVGFGLL
                     FPNTLIYIYFLLPIKAKYLVIILIAYELYTGFINDPNDNVAHFAHLGGALIGFIMIKY
                     WQKNSRRFY"
     gene            complement(4879..6681)
                     /gene="mutL"
                     /locus_tag="PLM90_08350"
     CDS             complement(4879..6681)
                     /gene="mutL"
                     /locus_tag="PLM90_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMZ89422.1"
                     /translation="MGDIIHLLPDSIANQIAAGEVIQRPASAIKELMENAVDAGATEI
                     KVVVKEAGKMLIQVIDNGKGMSFTDARMCFERHATSKIRSADDIFSIRTKGFRGEALA
                     SIAAIAQVELKTRQQEHELGARLVIEGFEVKAHEQAQCPVGTNIQVKNLFYNVPARRN
                     FLKSNTVELKHIIDEFERVVLAHPDIFFSLQHNGVELFHLKAGNLRQRIIHLFGSNYN
                     EKLVPISEDTSVTNIRGFIGKPDAAKKTKGEQFFFVNNRFIRSPYLHHAVMSAYQELL
                     PEGSYPLYTIYIDIDPARIDVNVHPTKQEIKFEDERIIYTFIQAAVRHALAQYSVTPT
                     LDFEQDQTFANLPSFYKKPEEQPVSFKTVESRMNDIPQHQSSFKHDVASTNRMHTAFK
                     REVSGALHDDEVQSDMPTDGATPFQIHYQYVVTQIKSGFIVIDQQAAHERILYEKYVT
                     QLREKKHHSQQQLFPETLKLSGADFALMEMLLPDLQNLGFDIMPFGKDAFVVHGIPVE
                     FENGDINGVVEALLEQFKEDKSTLKLDRHEAIARSLARTNSIKRGQKLDERGMRHLAD
                     QLFACETPYTSPFGNLTFVTFTLEALARQFQKSN"
     gene            6908..9877
                     /locus_tag="PLM90_08355"
     CDS             6908..9877
                     /locus_tag="PLM90_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HMZ89423.1"
                     /translation="MLTARLVIKSLLTAGMVSAAKRPAIPMPVDDTKVPYYEKDYPWV
                     DSLMQVMTLDQKIGQLFMAPAYSNKDEAHAKSIDNLVTKYQIGGLIFMQGGPVRQAKL
                     NNRYQKESKIPLLIGMDAEWGPAMRLDSVINFQRQLTWGAMSNDSIVYEVGTVIAQQL
                     KRLGVHVNFAPDIDINNNPNNPVIGDRSFGENKYNVALKGLMYMNALQDNHIVACGKH
                     FPGHGDVDADSHLTLPVVNHPYNHLDTVEFFPFKVLMNEGLGSIMLAHLYVPALDSTK
                     NQPSSLSPKVGRDLVRERLGFKGLIFSDALNMKGASAYYEDGELEVKAFLAGNDMLLF
                     SDNIPVAFEAIKKAVASGRITQAQIDESVKRILIAKAFAGLNVSKNKWVAEKNITDDI
                     NKPAATLIKRKITENSITLANAADTLIPFKKLDTLSIACISVSGGSKTTFQEYLAKYA
                     KVDLYQINKDADATAFVQSFEKLKNYDVVIVGVHDMKRARTSNYGITSNTENLVWKLS
                     RATKTVVCMFGTPYAIDKFSDAQYQIVSYDDDNYTQQATAMALFGAIPFHGRLPVGMG
                     KFKVNAGIETGSLQRIKYTIPEEVGIIPARLNRIDSLAKACIKVEAAPGCQVLVLKDG
                     NVIYDKAFGSYKYDNKDKVDVSSIYDLASVTKTSATTIMLMDLHDKGLLNLQKTVSDY
                     LPETEGTNIAGLKMQDILTHQAGLPAWVPFYKRTLNPDGTINPLYYSQTKIPGFTTQV
                     ADGIYMRDDYKDSVWNIIEHVDIKEKKYLYSDLSMYIGRRVAERIANVPLDVYLYQHF
                     YGPLGMQTTCFNPLQRFPKARIVPTENDNYFRYQTVQGYVHDMGAAMMGGVEGHAGLF
                     SDAEDLAILYQMLLNKGYYGGTQYFSPETVALWTKKQSSTSRRGLGFDKPEMNGPSPC
                     SGYASASAFGHQGFTGICVWVDPEYNLVYLFLSNRVQPKAEPNRLSTEGIRNKIMDVI
                     YEAILTGNPPKGSPASD"
     gene            10009..11148
                     /gene="bshA"
                     /locus_tag="PLM90_08360"
     CDS             10009..11148
                     /gene="bshA"
                     /locus_tag="PLM90_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HMZ89424.1"
                     /translation="MKIGIVCYPTYGGSGVIATELGKALADKGYKVHFITYKQPFRLD
                     SFHENIFFHEVNFTNYPLFEYPPYEIALSSKIVDVAINEKLDLLHVHYAIPHASAAYL
                     AKQILKDKNYHLPFITTLHGTDITLVGKDETFVPVVTFSINQSDGITSVSDSLKLDTY
                     QNFDIEKEIAVIPNFIDFTRFKKLNKEHFRKVIAKNGEKIVVHTSNFRKVKRVEDVVK
                     VFCKIKEAVPSKLLLIGDGPERQNVEKLARQVCLEEDVYFLGKQEAIEELLAIADLFI
                     LPSETESFGLAALEAMACEVPVISSNTGGIPEINIQGVTGFLSNVGDVEDMAKNAITI
                     LSNEDTLKQFRANAIKQAQAFSLENILPQYEAYYRKVVEESKSAR"
     gene            complement(11145..11558)
                     /locus_tag="PLM90_08365"
     CDS             complement(11145..11558)
                     /locus_tag="PLM90_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89425.1"
                     /translation="MKYFLPFLILLSGCSILGSHKSVYTPLNGDWYPIDQKFAGLQFP
                     ESTYATQRLMLRDSSYILSSEVVDSGKMICSDSTMDIYGIEGPNAGNHFMAIYKLRND
                     TLTISYNLTGGSYPDTFVSDSTNYYFVSIFVRAKD"
     gene            11682..12134
                     /locus_tag="PLM90_08370"
     CDS             11682..12134
                     /locus_tag="PLM90_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HMZ89426.1"
                     /translation="MSLFDQVNEGIKDAMKSKQEGRLRALRGIKAALLLAKTEKGAED
                     TLTEAKEMQVLTKMMKQREESYATYTQQNRPDLAAAEKEEMDVIAAFLPKQMSAEELR
                     AEVAAIITQLGASGMKDMGRVMGTATKQLAGKTDGDKIAAVVKEILGA"
     gene            12176..12718
                     /locus_tag="PLM90_08375"
     CDS             12176..12718
                     /locus_tag="PLM90_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HMZ89427.1"
                     /translation="MILDIIYAIILLLAMIHGYRKGIIHSIVSLLALIVGLLAAVRFS
                     ELAAVYIDKWFNISGKYLPLIAFIAVFIGLYLLFRLLEQALEGFFKLIKLNFVNQIIG
                     ALIWGLVWTMLFSTILFYLNNMGLFSDELKSDSVTYEKIAPIAPDTFELIGKIIPPVK
                     NTYNSLDEWFHKLHDNATSD"
     gene            12724..13293
                     /locus_tag="PLM90_08380"
     CDS             12724..13293
                     /locus_tag="PLM90_08380"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMZ89428.1"
                     /translation="MKILLLDNYDSFTWNLYDYLTRCGANVHVYRNDAITPQVISDMS
                     FNGIVLSPGPGRPQDAGIMMELIEKFHQTHPILGICLGYQALGAYFGAEVIHAPLPMH
                     GKTSTISHDGKGLFEGVPNPTSVMRYHSLNIDRMPPGIRLTAQTADTGEPMALAHEYL
                     PLSGVQFHPESVLTPAGLTMIGNWLKSMH"
     gene            13339..>13644
                     /locus_tag="PLM90_08385"
     CDS             13339..>13644
                     /locus_tag="PLM90_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ89429.1"
                     /translation="MTHTVTEENGFKYVEEGEGEVLMLLHGLFGALSNFFDIIEHFKA
                     THKVVIPILPLYELPIEETTVGGYVDYVERFVAYKGYGKVIVLGNSLGGHIALIYTLQ
                     "
BASE COUNT         4088 a         2821 c         2826 g         3909 t
ORIGIN      
        1 tgtctgataa ggcaaatgtg caagcagtat attgccttgc aaacacacct ataccttatg
       61 gcaacccttc aggtttgtct gcaaaaatta aaaatgaatc aacttccgcc gaaactttca
      121 ccgtaaactt taccgtaaaa gaaaaagtat ccggaacagt tcgttattcc agctcttcag
      181 gtgtttcatt tactgcagga gaagtgaaaa ctgttaccgc aacaggttgg aatccaacca
      241 ttcaggaaac agatactgtg attgttgatg ttcctgcttt gccgggtgaa acagttgttg
      301 ctaacaactc acttggttat cgtcacgtag ttaattcaga tacctatggt tatgccgatt
      361 taagtgctgc aaccggtgga gttggttttg gtgcaggtgc aggacaaata ttaaaccgac
      421 atacattaac aggatgcagc agtgttgaag caatcagagt atatattttt ggtgcagcga
      481 gtataggcaa taccattcgc ggtgtcgtaa atgatatcac cggtattcaa attggcgttt
      541 ctgccgatca cgtagtaacc gccggtgaag tcgacacatg gattacgctt cctattctgt
      601 cgccaccgtt tattactaac caggatgtgc tgtttggtat tgaacaaact gcaggcattg
      661 gcgctgctgc ctatttccct gttggtacac aaacagaatc cagcccaaca cgcgattttg
      721 cattttattc aaataacccc ggtggtggtg cgttagttgg tccttatact acgcttaacc
      781 gctggatgat tgaaggtgtt gtgacgccgt taagtacaga tcctactgtt tcaggtccta
      841 cagaagcctg cgatggtgat cctataagtt tcactgcttc atttactgaa ctcaaccctg
      901 ccgctgttgt taactggtac acagatggtt gtggaacaac gctcgttgga accggcatga
      961 cattctctac cactgcaatg gcaggaatga caacttacta cgttcgtgct gaagatgcat
     1021 gtaacggtgt aaatactgct tgtgcttcta tcactataac tgttggtgtt tctgaagttt
     1081 attttgctga tgccgatggt gatacttatg gtgacccggg tgctacagta ttaagttgcg
     1141 atggtgcgcc taccggatat gtacttgatc cttctgattg tgatgatact aactcatctg
     1201 tttatccggg tgctacagaa gtttgcgatg gtcttgataa taactgcgat ggcaatatcg
     1261 atgaaggtgt tgttgaagca gtgatcaccg ctccgggtgg actggtagca tgtaaaccat
     1321 tgtcggtttt attgcaaaca actgctattc cgggatatac ttatcagtgg tttaaaaatg
     1381 gtaatatcat aacaggagct attaacccaa catattctgc aagtaaacct gcatattatc
     1441 aagtacaagt aaacacaccg ggtggttgtt ttgcattgtc tgatgctgtg cttgtgaata
     1501 ttcttaatgc acctaatgca aacatcagcg caccaaacgg cacaagtctt tgtactactg
     1561 ttaagttgaa agccagctac gatgctacct atacttggca atggcgctta ggtggtgttg
     1621 acataggtgg agcaacaaac tacctgtatt atccatcaac tgcaggaagc tacacatgcg
     1681 ttgttacaag cgctgaaggt tgctcacgtg aaactgcttc aatcgctgtt actgcttgcc
     1741 gcgaaggtgg tgcaataagc gaagaaatta atactattaa tgtttatcca aatccttcag
     1801 caggaacatt cacacttgaa attatgtcag gtgacgaatt ctccggaaaa gctgatgtgt
     1861 atgtgatgaa tatgatcggt gaagttatat ataacaacaa tatcgatata actaacggct
     1921 atattaataa taccattact ctggctgacg gtatagctgg tggcctttat ttggtgaagg
     1981 taatggccaa tggccacgag tacacgcagc aagtggttat atccaaataa tcaaattgtt
     2041 ctgcgaaaca gaaaagaccc ggaagcgact ccgggtcttt tttatttgtt gcatttcttc
     2101 tatgcctata tgcaaggaat taaagattaa ttgaagccgg tattatcctt gaaagcgatc
     2161 ttgaaataag ttaatcttta gcggggatgc tgaacttagt aatgcaaggg taatgatcac
     2221 tcgatttatc ttttatagtg tagtaatcgt gaatatcaaa agcatcatct ttaaataaca
     2281 taaaatcaat gcgtataaac gggaacggcg catatgttgc tccaacacca aaaccgcgtt
     2341 ttaaaaaagc gtcttccatt ccgtgcgaca ttttatgata tgtgtaactt acaggggtgt
     2401 cattaaaatc gccacacgaa atcatcggaa atggtgatgc ttttatatgt gtggctacag
     2461 tgtcagcttc ctgtgcacgc aaagaaaaac tcatttttaa ttttgttgtg atcctgtcgg
     2521 catcaaaatc agtctccgct tcattgctgg cttcaatgaa ttccagatca taatgattga
     2581 gttgcgtgct ttgcatgtga ctgcaaaaaa cgcgtacggt atcattttca atcaacagat
     2641 cggcgtaaat ggttgtattc agcggactta atgaaagata ttctaaatga cctgtatcaa
     2701 ttattggaaa tttagtcata atcataaggc cgaaacttct ggtcgttttc ggattttcaa
     2761 atgctttata gtaatagtaa ttttttaacc cggttgccgt catcaccatg tcaagattac
     2821 tcttttcctt tctgtgattg gtgtaagtgt tgaattcttc catacacaat atatccggct
     2881 tggcatttgc aatattggca atgatctgat cgcggttctt atacttacct tccttgttgt
     2941 aagcatcgaa tccttgcaca ttatattgca tgagggtgta aaccggtttt tcactgtcgg
     3001 aagatcgctt taaaccacca agactaaagt ggcgaagatg ggctgtaaaa cctaaagtta
     3061 aaatgatcaa catgccataa aaaatcttcc tccgcataaa aagccacaac aacatgaaaa
     3121 acatatagca gagtaaaatg ggaaaatatc ccagcccaag aaaagctaaa ggccaaaatt
     3181 tctccggact gatatacata gccagcattg cgccaattag taacaaggcg aaaattatgg
     3241 ttatgaataa tatcaatttc tgccaccagc gcatattact tttctttgct cattctgaat
     3301 aggaaatctt tttcttgcct gcttaaactt tcatacccac tggcttttat tttatcgagt
     3361 atggcgtcaa gttttttttg atcttcaatt tctttagctc tgcgatcatc ggtatcctgg
     3421 cgctgataat tataggcaac gcgcaactta ttgcgatgaa atatttgcca tacccgagat
     3481 tcaaaatgcg gccaccaact gaaccaattg cccgactttc tataactgcg aatatacaaa
     3541 tagccaaaca atgctccgcc taaatgggca atatgtccac caatgttgct ttctcccgga
     3601 atacttatca catcaactat cacaacgaat gccgcgatat atttcaattt cacagggcct
     3661 aaaaagtaca aaaacacggt gtaatttggc attaaggtgg tggtagcaat tacaattgcc
     3721 atgatagccg ctgaggcacc tacaaaatac cccccaatat tttcctgata gcccggaaaa
     3781 agataaaagg aaattaatgc aagaaaaaat ccggcgaaac ctccggcaaa atataacgga
     3841 taagcaagct tttgattggt gatgtcactg aaaatgcgtc caaagaaaaa taaatagatc
     3901 atattggaaa caatatgaaa tatattctga ggatcatgca tgaacatgta gctgaccaaa
     3961 gtccatgggc gagttgcgag tgtactgata ttcgaattcg cccagaacca gtttacgaga
     4021 aaatccagat aggcttcttt ttgatgaaat ccataagcaa caagcaggcc taggctttga
     4081 gccaggtaaa tagccacgtt gacaataatc agcttcatgg ctgcagtgcc atgttgccat
     4141 tgatatttta tatcatcgaa aaatgccatc tatgcaccaa gggtgattaa tagaaacgcc
     4201 ttgaattttt ttgccaatat ttgatcatta taaatccaat caatgcgcca cctaaatgtg
     4261 caaagtgcgc aacattgtcg ttaggatcat tgatgaaacc ggtatataat tcgtaggcta
     4321 tcaaaatgat gacaagatat ttcgccttga tcggaagaag gaaataaata taaatgagcg
     4381 tattggggaa taacaagcca aaacctacca ggataccata gatggctccg gatgccccta
     4441 ccgcactgta gttcaagcca tttacattcc agcgcaaagg cgtatcagta atggatgaag
     4501 aagggaaaat ggtgccgact gattgatata cttcataggc atgatacgca gtaaaacata
     4561 atgctgcacc taaaccggta attaaaaaat attgcaaaaa tcgttttgga ccccacacat
     4621 tttctaatac tgccccaaac atccacaaac tgaacatgtt gaaaaacaca tgcgcaaagt
     4681 tttcatgggt aaacatatgc gtcacgattt gatatggcct gaattccggt gtcgacggat
     4741 agtgcaacgc aagcgtttcc ttcaattccg gaaatgtaaa agttgcaaga tataccaacc
     4801 cattaatgat caatagattt ttgatcaccg gcggcagata attgaacctt gtaaattgta
     4861 tttgcgacat gttattgttt aattgctttt ttgaaattgt cgtgccagtg cttcaagggt
     4921 aaaggtgaca aaagtgagat tgccaaaagg agaagtgtat ggcgtttcac aggcaaataa
     4981 ttgatctgct aaatgacgca tgccccgctc gtccagtttt tgtccgcgct tgatgctgtt
     5041 tgtacgtgca agtgatcttg caatagcttc atggcgatcg agcttgagtg ttgatttatc
     5101 ctctttaaac tgctctaaca atgcttccac tacgccattg atatcgccgt tttcaaattc
     5161 aaccggtatg ccatgcacta caaaagcgtc tttgccaaag ggcatgatgt cgaagcccag
     5221 gttttgcaga tcaggaagca acatttccat caacgcaaaa tcggcacccg ataattttaa
     5281 tgtttcagga aataattgct gttgcgagtg atgctttttc tctctcaatt gcgtaacata
     5341 cttttcatat aaaatgcgct catgtgccgc ttgctgatca attacaataa atcccgactt
     5401 tatttgagtc actacatatt gataatgtat ttgaaatggt gtagcgccat cggttggcat
     5461 atccgattgc acctcatcat catgcaatgc acccgaaact tctcgtttaa atgctgtatg
     5521 catgcggttg gttgatgcca catcatgttt aaatgacgat tgatgctgcg gaatatcgtt
     5581 catcctgctc tcaacagttt tgaaactcac cggctgctcc tcaggctttt tgtagaaact
     5641 tggcagattc gcaaacgttt gatcctgctc gaaatcaagt gtcggcgtta cgctatattg
     5701 tgccaaggca tgtcgcaccg ccgcttgaat gaaagtatat ataatgcgct cgtcctcaaa
     5761 ttttatttcc tgtttggtag gatgcacatt cacgtcgatg cgcgccggat cgatatcaat
     5821 gtaaatggta tacaatggat aacttccctc gggtaacaac tcctggtatg ccgacattac
     5881 ggcatgatgt aaatacgggc ttcgaataaa tcggttgttg acaaaaaaga attgttctcc
     5941 cttggttttt tttgctgcat cgggtttgcc aatgaaacca cggatattag taactgaggt
     6001 gtcttctgaa attggcacca gcttctcatt gtaatttgaa ccaaacaaat gaatgatgcg
     6061 ctgacgtaga tttcctgcct tcagatgaaa taattctact ccattatgct gcagactgaa
     6121 gaaaatatca ggatgtgcta aaacaacacg ctcaaactca tcaataatat gtttaagttc
     6181 aacggtgttc gatttcaaaa agttgcgcct ggcgggaaca ttataaaaaa ggtttttcac
     6241 ctgaatattt gttccaaccg gacattgagc ttgctcatgt gccttaacct caaaaccttc
     6301 aatcactaac cttgcaccta attcatgctc ttgctgacga gtttttagtt ctacctgggc
     6361 tatcgccgcg atactggcaa gcgcttcgcc tcgaaatccc ttcgtgcgaa tagaaaaaat
     6421 gtcatcggca ctgcggatct tactggtggc atgcctttca aagcacatcc tggcatcggt
     6481 aaaactcata cccttgccat tgtctatcac ctgaataagc atttttcctg cttccttcac
     6541 aacaaccttg atctccgttg ctcctgcgtc tacggcgttt tccatcagct ccttgatagc
     6601 cgaagcaggg cgttgaatta cctcacctgc agcaatctga ttggcaattg aatccggtaa
     6661 aagatgaata atgtctccca tggctaattc actccagacg cgcagctcac aagtccactc
     6721 tcccgcctga attgcaaaag taatgtttta cgggtggcta atgtctgaat tagtatgcta
     6781 tttctgcgct tgaccacaac attagggtag gaattatcgt tccagcggtt accttgacca
     6841 ttatttttgc aaggaaagtg aaaacaaggc atacttttgg tttcgtattg cccgaaagca
     6901 ttcaagcatg ttaacagcac gactcgtcat aaaatcgctt ctcaccgccg gaatggtgtc
     6961 tgccgcaaaa cggcccgcca tacccatgcc tgttgatgat accaaagtgc cttattacga
     7021 aaaagactac ccttgggtcg attccctgat gcaggtaatg accttggacc agaaaattgg
     7081 tcagcttttt atggcacctg cctattcaaa taaggatgaa gcgcatgcaa aatctatcga
     7141 caacctggta acaaaatatc agatcggcgg actcatcttt atgcagggcg ggccggtccg
     7201 tcaggcaaaa ttgaataaca ggtatcagaa agagtcgaaa ataccacttc tgataggtat
     7261 ggacgccgaa tggggacctg ccatgcgcct cgattctgtc atcaacttcc agcgccagct
     7321 cacctggggc gccatgagca acgatagtat cgtgtatgaa gtgggaaccg ttattgccca
     7381 acaactaaaa agacttggtg tgcacgtcaa tttcgcaccg gatatcgata tcaataataa
     7441 tcctaataac cctgtaattg gcgataggtc gtttggagaa aataaataca atgtggcgct
     7501 taagggcctg atgtacatga atgcccttca agacaatcat attgttgcct gcggaaaaca
     7561 ttttccgggg catggtgatg ttgatgcgga tagccatctt acattgcctg ttgtaaatca
     7621 tccatataac catctggata ccgttgaatt tttccctttc aaagtcttga tgaatgaagg
     7681 gctcggatct atcatgttgg cacatttata tgttcccgca ctggatagta ccaaaaatca
     7741 accttcatcc ctctcgccaa aagttggtcg cgatctggtg cgcgaacgtc tcgggttcaa
     7801 gggtctcata ttcagtgatg cgcttaatat gaaaggtgcc agtgcttatt atgaagatgg
     7861 cgaattagaa gtaaaagcat tccttgccgg caacgacatg ttgttgttca gcgataatat
     7921 tcccgttgct tttgaggcaa taaaaaaagc agtggcatcg ggaagaatta ctcaggcaca
     7981 aattgatgaa agcgtaaaac gcatcctcat cgcaaaagcc ttcgcaggat taaatgtcag
     8041 caaaaataaa tgggtggcgg aaaaaaatat caccgatgat attaataaac cggctgctac
     8101 attgattaaa agaaaaatta ctgaaaatag tattacactt gcaaatgctg cggatacatt
     8161 aataccattt aaaaaacttg atacattatc tattgcatgt atcagtgttt ccggtggaag
     8221 taaaacaacc tttcaggaat atcttgctaa gtatgcgaag gtagatttat atcaaataaa
     8281 taaagatgca gatgcaacag catttgtgca gtcgttcgag aagttgaaaa attatgatgt
     8341 agttattgtg ggtgttcatg acatgaaaag agcgcgaact tctaactacg gcattacctc
     8401 aaatacagaa aacctggtat ggaaattatc gcgtgctaca aaaacagtag tatgtatgtt
     8461 tggcacacca tatgccattg ataaattcag tgatgcacaa taccaaattg tatcctacga
     8521 cgacgacaac tatacccagc aagctactgc tatggcgctt tttggcgcaa ttcctttcca
     8581 tgggagatta cctgtgggca tgggaaaatt taaggtaaac gcaggaatag aaacgggctc
     8641 acttcaacgt attaaatata ccatacccga agaagttggt atcattcctg ccagattaaa
     8701 cagaatcgat agccttgcaa aagcatgtat taaagttgaa gcggctcccg gatgtcaggt
     8761 gcttgttttg aaagatggca atgttatcta cgataaagca ttcggtagtt ataaatatga
     8821 taataaagat aaagttgatg taagcagtat ttatgacctc gccagtgtta ccaaaacatc
     8881 agcaacaacc atcatgctca tggatctgca tgataaaggt ttgttgaatt tgcagaaaac
     8941 agtttctgat tatttacccg aaacagaagg aacaaatatt gccggtttaa aaatgcagga
     9001 tatccttacg catcaggccg gattacctgc ttgggtgcca ttttataaac gcacacttaa
     9061 tcccgatggc actataaatc cattgtatta tagtcaaacc aaaatccccg gattcaccac
     9121 acaagttgca gatggtattt atatgcgcga cgattataaa gacagcgttt ggaatattat
     9181 tgagcacgta gatatcaaag aaaaaaaata tttatacagc gacctgagta tgtatattgg
     9241 tcgacgggta gctgaacgta ttgcgaatgt accattagat gtttatttgt atcagcattt
     9301 ttatggtccc ttgggaatgc aaactacttg ctttaatccc ttgcagcgtt tccccaaagc
     9361 gcgcattgtt cctactgaaa atgataatta tttccgctac cagaccgtgc aaggatatgt
     9421 gcatgatatg ggcgctgcca tgatgggagg ggttgaaggt catgccggtt tattcagcga
     9481 tgccgaagac ctggccatat tgtatcagat gctgctcaac aaaggttatt atggcggcac
     9541 ccaatatttt tctcccgaaa cagttgcctt atggactaag aagcaaagta gcacgtctcg
     9601 tcgcggactt ggcttcgata aaccggaaat gaacggacca tcaccatgca gtgggtatgc
     9661 ctctgcttct gcttttggcc accagggatt taccggaatt tgtgtgtggg tagatcctga
     9721 atacaatctc gtttatctat tcctttccaa tcgcgttcaa ccaaaggcag agcccaaccg
     9781 tttgagcact gagggtattc gaaataagat tatggacgta atctacgagg caattctcac
     9841 tggaaatcca cccaaaggaa gcccggcttc tgattgaatt tctgtagcca cttgtgtatg
     9901 attgaacact gatttctcca ttgggttgct tggcaatcgt ggccttattt gtcccgcgtt
     9961 gaacttttcc ggctaaattt tcattctact tctcattacc ttcacaacat gaaaattggg
    10021 atagtttgtt accccacata tgggggaagt ggtgttatag caacggaatt agggaaagca
    10081 ctggcagaca aaggctataa agtgcacttc attacctata agcaaccctt tcgtttggat
    10141 tcctttcatg aaaatatttt tttccatgaa gtgaatttta ctaattatcc cttgtttgaa
    10201 tacccaccct atgaaattgc actaagtagc aaaatcgtgg acgtggctat caatgaaaaa
    10261 ctcgacctct tgcacgtgca ttatgctatt cctcacgcat cagctgctta tttggcaaag
    10321 caaattttaa aggataaaaa ttatcatctg ccatttatta ctactttgca tggtactgat
    10381 atcacacttg taggaaaaga tgaaaccttt gttcctgtag ttactttttc catcaaccaa
    10441 agcgatggca ttacgtccgt ttctgatagt ttgaaattag atacttatca aaactttgat
    10501 atcgaaaaag aaattgctgt cattccaaac tttattgatt ttacacgctt caagaaactc
    10561 aataaagagc attttagaaa agtgattgca aaaaatggtg agaaaatagt ggtacatact
    10621 tctaacttcc gaaaagtgaa aagggtagag gatgtagtga aagtattttg taaaataaaa
    10681 gaagcagttc cttcaaaatt actcctcatc ggcgatggac ctgagcgtca aaatgtagag
    10741 aaacttgcgc gtcaggtttg tctcgaagaa gacgtgtatt ttttaggaaa acaagaagct
    10801 atagaagaat tattggctat tgccgattta tttattcttc catcagaaac cgaaagtttc
    10861 ggtctggctg ccctcgaagc aatggcatgt gaagtgccgg tgatatccag caatacaggt
    10921 ggtattccgg aaattaatat tcaaggtgtt acaggttttc tcagcaatgt tggcgatgta
    10981 gaggatatgg cgaaaaatgc cattaccata ttgtcaaacg aagacacttt aaagcaattt
    11041 cgtgcgaatg caataaaaca ggctcaggct ttttcacttg aaaatattct gccgcaatac
    11101 gaagcctatt atcgcaaggt ggttgaggaa agtaaatctg ctcgctaatc cttggcgcga
    11161 acaaatatcg aaacgaaata atagttcgtc gaatctgaaa caaatgtatc aggatacgac
    11221 cctcctgtca aattatagct gatggtcaat gtatcattgc ggagtttata aatcgccata
    11281 aaatgattac ctgcatttgg tccttcaata ccataaatgt ccatggtgct gtcgctacag
    11341 atcattttcc ccgaatcaac aacttccgac gataaaatat aactcgaatc acgcaacatc
    11401 aatcgctgcg tggcataagt tgattccgga aattgtaatc cggcaaattt ttgatctatc
    11461 ggataccagt cgccattcaa cggcgtataa actgatttat gacttcctaa aatcgaacat
    11521 ccggataaca aaatcagaaa tggtaaaaaa tatttcatat cactaaattt tcggggaata
    11581 ttctatttaa aacaattttg ctgatgtggg cacgaaggta tactattttc taaaattgag
    11641 cttgtcactt tttcatgtaa ctttacacta aaataaaaaa tatgtcactt ttcgatcagg
    11701 taaacgaagg cataaaagat gctatgaaaa gtaaacagga aggacgcctg agggcacttc
    11761 gcggaattaa agcggcgctc ttattggcaa aaactgaaaa aggtgcggaa gacaccctca
    11821 cagaggccaa agaaatgcag gtcctcacta aaatgatgaa gcaacgcgaa gaaagttatg
    11881 ccacctatac acaacaaaac agaccggatc ttgcagctgc agaaaaggaa gagatggatg
    11941 tgattgctgc tttcctgcca aaacaaatgt cggctgaaga attgcgtgcg gaagttgcag
    12001 ccatcatcac acaattgggc gcaagtggca tgaaagatat gggacgagtt atgggcactg
    12061 caaccaaaca acttgcaggc aaaaccgatg gcgataaaat tgccgccgtg gtgaaagaaa
    12121 tccttggtgc ctaagtgcag gcatttgttt ttatttcaaa aaataagcgc tcaatttgat
    12181 actggatatt atttacgcaa taatcctttt gttggccatg attcatggat acagaaaagg
    12241 tattattcat tctatagtat cgctgttagc acttattgtc ggattacttg ccgccgtccg
    12301 atttagcgaa ctggcagcgg tatatattga taagtggttc aatatttccg ggaaatatct
    12361 gccactgatc gcttttatcg ccgtttttat agggttatat ctattattca gattgctcga
    12421 acaagcactt gaaggctttt ttaagcttat caaactgaat tttgtaaacc agataattgg
    12481 ggcattgatt tggggattag tatggactat gcttttctct accatattat tttacctgaa
    12541 taacatggga ctgttttccg atgaattgaa atcagattcc gtcacctacg aaaaaattgc
    12601 tccgattgct ccggatactt ttgagttgat agggaaaatt attccgccgg taaaaaatac
    12661 ctacaacagt ctcgacgagt ggtttcataa acttcatgat aacgcaacaa gcgattaaca
    12721 tgcatgaaaa tactgctgct cgataactac gattcgttta cctggaatct ttacgattac
    12781 ttgacccgct gtggtgccaa tgtccatgta tatcgaaatg atgccattac cccacaggtc
    12841 ataagtgata tgtcattcaa cggcatagtg ttgtctccgg gtcccggaag gcctcaggat
    12901 gccggaatta tgatggaatt gatcgaaaaa tttcaccaaa cccacccaat tttaggtatt
    12961 tgcctcggtt atcaggccct gggagcctat tttggtgccg aggtaatcca cgcccctctt
    13021 cccatgcatg gaaaaacctc aaccatatcg cacgatggaa aagggctctt tgaaggcgtg
    13081 ccaaacccca cgtccgtaat gcgctatcac tccctgaata tcgaccgaat gcctccgggg
    13141 ataaggctta cagctcaaac tgccgatacc ggtgaaccca tggctttggc acatgagtac
    13201 ctgcctctta gcggtgttca gttccaccct gaatcggtgt tgacacccgc cggactaacc
    13261 atgatcggca actggctgaa aagcatgcat tagcaaacct gacaataaat gctatttttg
    13321 taacgcactc aacacggtat gacgcacact gtcacagaag aaaacgggtt taaatatgtg
    13381 gaagaaggcg aaggtgaagt actgatgctt ctgcacggcc tattcggcgc cctcagtaat
    13441 tttttcgaca tcatcgaaca ctttaaagcc acgcataagg tggtgatacc catattgcca
    13501 ctatacgaat tgcctatcga agaaactaca gttggcggtt atgtcgacta tgtagagcgc
    13561 ttcgttgcct acaaaggtta tggcaaagtg attgtacttg gaaattcact cggaggccat
    13621 atcgccttga tctatacact ccag
//
LOCUS       DAOQOU010000082        13637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2324_length_13637_cov_5.502062, whole genome shotgun sequence.
ACCESSION   DAOQOU010000082 DAOQOU010000000
VERSION     DAOQOU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13637
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2324_length_13637_cov_5.502062"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLM90_08390"
     CDS             complement(<1..760)
                     /locus_tag="PLM90_08390"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HMZ89430.1"
                     /translation="MIELPVTESQERQRKPKWLKVKLPTGENYRMVRELVDQHKLHTI
                     CESGNCPNMGECWGEGTATFMILGNVCTRSCSFCAVATGRPTELDLEEPFRVAEAIKL
                     MGVKHAVITSVNRDELADRGAAIWNRTVTEVKRLSPQTTIETLIPDVKSNWDALEMMI
                     SPGQEVVSHNMETVKRLYRLVRPQAKYERSLEQIKRTKDYGKRTKSGVMLGLGESAEE
                     VYEIMDDLVANGCEILTLGQYLQPTRMHLDVQEYV"
     gene            complement(779..1585)
                     /locus_tag="PLM90_08395"
     CDS             complement(779..1585)
                     /locus_tag="PLM90_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89431.1"
                     /translation="MKFVFFCMAWMAVVYSAVAQDTSTAKQNTSKPVILNKSSASKPK
                     GLSYEHEFSMGAKVTTTGWGIFGDYTKDIDLDKKRVAYFELEFLKDPKETKRVNEFTV
                     GYTPPKPFVYGKQNTFFNLKAAIGEKRMIGEKAEKSGFQVAANYAGGFSAGLVKPYYL
                     AVYSTSEDGTPTENVKYTPETESLFLDYTSIYGASGFSIGFNEISFVPGLFGKAGLNF
                     DWATYDDFVKSAEAGIGAELYLKDIPIMILEDNKPYFVYLYLSLQFGKKW"
     gene            1644..2048
                     /locus_tag="PLM90_08400"
     CDS             1644..2048
                     /locus_tag="PLM90_08400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ89432.1"
                     /translation="MITSQVTYEGELRTTAVHTLSGTSIITDAPPDNQGKGEAFSPTD
                     LVATALASCMLTIMGIAARTHEMDIQGTRVEVTKIMASNPRRISEVHCHLFISGNFND
                     KQKAILEHAARTCPVIQSLSSDMIKVVTFSYA"
     gene            complement(2103..2243)
                     /locus_tag="PLM90_08405"
     CDS             complement(2103..2243)
                     /locus_tag="PLM90_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89433.1"
                     /translation="MRTFSKWAVAAGLCLLLTAGLSSCGHKEGCPNKITEVGGIKIET
                     NA"
     gene            2408..3748
                     /locus_tag="PLM90_08410"
     CDS             2408..3748
                     /locus_tag="PLM90_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HMZ89434.1"
                     /translation="MRIHLIAIGGAVMHNMALALHAVGHQVSGSDDEIFEPSKSRLSR
                     KGLLPEAFGWFPERITSELDAVILGMHARADNPELLRAQELGIRVYNFPEYVYEQSKN
                     SLRVAICGSHGKTSITSMIMHVLKKRRMPFDFLVGAQLEGFDTMVQFSQAPVTIIEGD
                     EYFASPLDKRPKFLFYHAQVKVISGIAWDHFNVFPTFDNYVDQFRQLVNQSTENDILI
                     ANADDEEVITLLDISRPKGKIIKYGAPAYKVIEGSFTLDVDGAHYQFAIFGRHNMQNM
                     EAAKNVCISLGISAHEFYEAMQSFTGAAKRLEVLKRSSDHIIFRDFAHAPSKVKATVD
                     AVREQFPDQPLVACLELHTFSSLNKDFIAHYHGTMQAADKRIVYFNPHTLTLKKLPML
                     SIEEVRSFFGDKELIVLNDSSSLKSELQSLNSVNNNILLMSSGNYDNLDLTFLT"
     gene            3757..4539
                     /locus_tag="PLM90_08415"
     CDS             3757..4539
                     /locus_tag="PLM90_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ89435.1"
                     /translation="MAQLQLDKPLAFIDLETTGINLGSDRIVEISILKLFPNGNRETK
                     TMRINPGIPIPAESSAVHGIYDADVADKPTFAQVADELNAYLDNCDLAGYNSNKFDVP
                     MLVEEFLRCGKGFDENRRYIDVMRIFTLMEKRTLEAAYRFFCDKTLENAHSAEADVTA
                     TYEVLLGQLDKYGDALAHDVPSLHEFTKDGDFVDFGRRMIMKNGVPCFNFGKYNGQSC
                     EDVYRREPQYFDWIYKSDFPEHTKLKLRLIALKIKNDKNKAS"
     gene            4544..5272
                     /locus_tag="PLM90_08420"
     CDS             4544..5272
                     /locus_tag="PLM90_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HMZ89436.1"
                     /translation="MARALVIIPTYNEQANIADMITAVLSLPQDFDLLIIDDNSPDGT
                     ASAVTAMQSTYPTRLHLHQRAGKLGLGTAYIAGFKWGLAQGYDYMIEMDADFSHKPED
                     LPRLLDACKNGADVAIGSRYIKGGNVEDWPTDRILLSKWASRYVRMITWIPVHDTTAG
                     FVCYKRNVLDTIDLDKIRFIGYAFQIELKYAAWRSGFSLKEIPIVFKDRQKGISKMSK
                     GIFKEAVYGVITMRWKSFFHSYTK"
     gene            5279..6361
                     /gene="corA"
                     /locus_tag="PLM90_08425"
     CDS             5279..6361
                     /gene="corA"
                     /locus_tag="PLM90_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMZ89437.1"
                     /translation="MSSEQRHKRKGIHRKKSGLPPGTPIYTGAKKHEIITLQRFAYDT
                     DTIEEINCKAGDKIDLIPREGKVLWVNINGLHQEALIEEIAATFKLHNLVLEDILNVF
                     QIPKAEEYPDTDQIYIALNEFYFAAEGKLERDQISIILGNDYVLSFQEEEGDYFELIR
                     DRLRNAKGKLRKKSGDYLVYVLIDSVVDSYYVVLDHYATELDSIENDIFEKKNRNHLE
                     RIHQLNKDLIYLRRSIAPLKEVLFKLLKEDVILINPESKLFLRDVLDHVHQVVNQIDV
                     DREYLADLVQTNMANMNAHLNEIIKVLTLISTIFIPLTFIVGVYGMNFGNIPLMQTTY
                     GYFVVWGVMIFITLLQFFIFKRKKWL"
     gene            6392..7189
                     /locus_tag="PLM90_08430"
     CDS             6392..7189
                     /locus_tag="PLM90_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89438.1"
                     /translation="MRLTLPIFISACILTSNLQAQSTIAPPANTSVLFNINFDLGIPM
                     NEFKTYNDEVAAGGGFSLFFQPSNKIPVLVGFDMAFMGNGHKTQHETLTADIVAGTTV
                     IETLYFPMRVETYNMITTGDVHLRVQSPTNFFKPYLDGMVGFNHFGTNTSVYDESEEY
                     YLSEEDNPLITSSQQNSSWSLNYGGALGMLVQLNENIFLDVRCAYTFGGEAEYYVEDD
                     IENWEIEFNTVPTSQDDVSDEDIDISAIKKQSHTDMIMGTIGLTFKF"
     gene            7316..7945
                     /locus_tag="PLM90_08435"
     CDS             7316..7945
                     /locus_tag="PLM90_08435"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HMZ89439.1"
                     /translation="MRSGRRKLSHKVHLVYGLVVIIAVGIAFFILHNLKASNASQLND
                     SSATPVIDSLHHIPVYYNGDVDHVAGRSVARNGYNYGLKWQCVEFVKRYYADHLGHYM
                     PDADGNAKDLFDKRLPDASFNEARALVQYANPSLKKPQPDDIIVFDGHLGNRYGHVAI
                     ISQVTETSIEIIQQNAGPDTPTREWLSLEKVDNRWEVDHKHVLGWLRVE"
     gene            complement(8014..8523)
                     /locus_tag="PLM90_08440"
     CDS             complement(8014..8523)
                     /locus_tag="PLM90_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HMZ89440.1"
                     /translation="MKKLITLVFCAAVGFNTAHAASLPEPEDESTKLNLWIPGVVMRI
                     AADIVEDHMDAEGAATGALLRKFGSINICIREGEAYADRTDKKITRKLDRMERRDYEQ
                     LMSVRTDEETVNISIRSNKHDKIKRMVLLVDEKEETFVYLKMHCRLKVEDIPQLVQSM
                     NFDDDDSDK"
     gene            complement(8548..9069)
                     /locus_tag="PLM90_08445"
     CDS             complement(8548..9069)
                     /locus_tag="PLM90_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HMZ89441.1"
                     /translation="MKKMLFTACALMISAMAFAQSAQIDQFFKKYEGKEGFTSVIVTE
                     KLFSLVASATTDDADLKNMIDGIKGIRILVYENAEGASNSGAYYNEFMTTVGTNGYEE
                     LMNVNAEGEKVRLYGKNITGSTLNDMLMVCDADGEFVLISILGAIDLSKISQISDIDI
                     EGLDELKKVEETK"
     gene            complement(9184..9657)
                     /locus_tag="PLM90_08450"
     CDS             complement(9184..9657)
                     /locus_tag="PLM90_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89442.1"
                     /translation="MADYLQTGITTVKELFQFLADRSTSRDRIKNALLRELRDNMKLL
                     EHRDKSGVNVMAMIDGLSASAIAEAYSANYKFDTLCEGPKKLPAAVILSKQQEKYIGW
                     NVERFMYAIEGKIRDLKNLPKLYSNLETAPVNLSVRLNNLYNQMLLLVIFISRKA"
     gene            complement(9682..10158)
                     /locus_tag="PLM90_08455"
     CDS             complement(9682..10158)
                     /locus_tag="PLM90_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMZ89443.1"
                     /translation="MHAQLTPWWQEQQVDSTEIKELAKADSIIAYAKTFIGTPYVWGG
                     TDPQHGFDCSGFICYVYKAFNITLPRTSDMQFDAGIPIPYVDARPGDIIVFSSATVVP
                     GDPGHVGIVLDYDAENGFSFIHTSSPETGGVRISNEATEKYYNKHFLEIRRVITAN"
     gene            complement(10215..10685)
                     /locus_tag="PLM90_08460"
     CDS             complement(10215..10685)
                     /locus_tag="PLM90_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HMZ89444.1"
                     /translation="MSKKEKKEEVVVRKLTPVLPDHLFVARTPDKGRAVFTSKAVKAG
                     DTLEVAPMIVFSEKDRLSIDDTFLYEYYFEWGKKGKKGALALGFGSLYNHSYSPNARY
                     IPDFDLNVLEFQAVRDIEPGEEITVNYNLDPEDQTPVWWERDAKKKKKKKSQHP"
     gene            complement(10693..10944)
                     /locus_tag="PLM90_08465"
     CDS             complement(10693..10944)
                     /locus_tag="PLM90_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89445.1"
                     /translation="MSKRNAKKRIGAAVASMIDRVLFMDITDEQLESKINALFNTYEA
                     AVKKINGSSKVKGKSAVKKHFTALFADVKKQLEDVVSAK"
     gene            11004..11315
                     /locus_tag="PLM90_08470"
     CDS             11004..11315
                     /locus_tag="PLM90_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HMZ89446.1"
                     /translation="MFDIKKAMEMKKQMEEIQKRLDNITVEGVSGDGKFQVVVTITAN
                     KKVKSVHISDELMASGDKEHLEDLLVIAMERGLDKATQVSEAEARNVALSGGLNGLFG
                     S"
     gene            complement(11316..12374)
                     /locus_tag="PLM90_08475"
     CDS             complement(11316..12374)
                     /locus_tag="PLM90_08475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HMZ89447.1"
                     /translation="MFKGLRIIELAGVMAGPAAGSFFSELGADVVKIENPRTGGDITR
                     QWKLPSENPAASTSAYFAAVNWRKLHLFLDLEDPSCLEQVYDMIRQADVMICNWKKGD
                     AQKFKLDPNTVAALNPGIIYANINGFGDEEDRVAFDVVLQAETGWMFMNGTPSSPPVK
                     LPVAIIDLFASHQLKEGILTALIQKYKTGKGSRVSVSLFDASIAALANQAANYITAGH
                     IPQRNGSLHPNIAPYGEILTCKDGVQVVLAIGSDRQFKNLCDILNCPEVYADQRYAHN
                     TDRVHNRNTLIGVLQSKVQSYSSDSFLELCRQNHVPVGAIRDLQQVFELPSAKELILS
                     DDAGKRVKSAIFKVEELV"
     gene            12609..>13637
                     /locus_tag="PLM90_08480"
     CDS             12609..>13637
                     /locus_tag="PLM90_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ89448.1"
                     /translation="MLRRLLPIIIFVIGADIVSIQAATNIFGSSTLLYGIWTGATVLV
                     LASLFLYFSREMRKRSGIARPVLMSTFFMLLFPKVIVSVLLLIEDVVRLTKLMANGVI
                     TALGSDFNFGLGRSFWWDAFALSFALLLTLAFVYGAIFNVYRYQIRKVKVKLVKLPKA
                     FDGLRVVQISDIHSGSLSNREAVAKAVDRINSLSPDLIFFTGDLVNNIAEEARGFVDI
                     FAKLQAKHGVYSILGNHDYGDYMAWPDEATKQHNLETLKTMHAQMGWRLLLNEHVHIE
                     RSGNKIAVAGVENWSARGRFKSYGRLEQALHGIQDAMTVLLLSHDPSHWEGEVLDHPA
                     KVDITFSGH"
BASE COUNT         3866 a         2765 c         2974 g         4032 t
ORIGIN      
        1 taacatattc ctgcacatcc aggtgcatgc gtgtgggttg gagatattgg cccaatgtga
       61 gtatctcgca gccatttgca accagatcat ccattatttc atatacttct tccgcacttt
      121 ctccaagccc cagcattacg cctgattttg tgcgcttgcc ataatctttt gtgcgcttga
      181 tttgctccag gcttctttca tattttgcct gaggtcgcac cagacggtat aagcgcttaa
      241 ccgtttccat gttgtgactg actacttcct gtccggggga tatcatcatt tccagggcat
      301 cccaattact tttaacatca ggaatcagtg tttcaatggt ggtttgcgga cttaagcgct
      361 tcacttcagt taccgttcta ttccagatag cggctcctct atcagccagc tcatcgcgat
      421 ttaccgaggt gataacggca tgtttcacgc ccataagctt tattgcttcg gcaacgcgaa
      481 acggttcttc gaggtcgagc tctgttggcc ggccggtagc cacagcacaa aaagagcaag
      541 atcttgtgca tacgttaccc aaaatcatga acgttgcggt tccttcaccc caacattcgc
      601 ccatgtttgg acaatttcca ctttcgcaga tagtatgtaa tttatgctga tcgacaagct
      661 cgcgcaccat gcgataattt tcgccggtgg ggagtttaac tttaagccat ttcggctttc
      721 gctgtcgttc ctgagattct gtaaccggaa gttcgatcat gttgctgaca ccactatttt
      781 accacttctt accgaactgc aaacttagat ataaatacac gaaataaggc ttgttatctt
      841 cgagtatcat aattggaata tctttcaaat acagctctgc accgattcct gcctctgccg
      901 actttacaaa atcgtcgtac gtagcccaat cgaaattgag acctgccttt ccaaagagac
      961 cggggacaaa ggatatttca ttaaacccta tgctaaagcc tgaggcaccg tagatggagg
     1021 tataatctaa aaacagcgat tccgtttccg gcgtgtattt gacattttcc gttggcgtgc
     1081 cgtcttcgga ggtactgtac accgccaggt aataaggttt tacgagcccg gcactaaatc
     1141 cgccggcata atttgcagct acctgaaagc cacttttttc agccttctcc cctatcatgc
     1201 gtttttcgcc gatggcagct tttaggttga aaaacgtatt ctgtttgccg tatacaaaag
     1261 gttttggagg ggtgtatccg actgtaaatt cgtttacacg cttagtttct ttaggatctt
     1321 tgaggaattc caattcaaaa taggctacac gcttcttatc gaggtcgatg tctttggtat
     1381 aatcgccgaa gatgccccaa ccggtagttg tgacttttgc gcccatgctg aactcatgtt
     1441 catagctgag tcctttgggt ttagaagccg aacttttgtt gagaatgaca ggtttagaag
     1501 tgttttgttt cgctgtagac gtatcctgag cgacagcgga gtacaccact gccatccatg
     1561 ccatacagaa gaaaacaaat ttcatcgcat aatattacga tttttgcgga taaatagttg
     1621 cccaaagcaa aaaattaact gtcatgatta catcacaagt tacctacgaa ggcgaattgc
     1681 gcacaacagc agtgcacacc ttatccggaa cgagcatcat caccgatgca ccacccgaca
     1741 atcagggaaa aggggaagct ttttcgccaa cagatcttgt agctactgcc ttggcgagct
     1801 gtatgttaac gattatgggc atagcagcac ggacacacga aatggatatt caaggcacca
     1861 gggttgaagt gactaagatc atggcctcaa acccgcgccg tatcagcgaa gtacactgtc
     1921 atttgtttat ttccggcaat ttcaacgaca agcaaaaagc cattttggaa catgcggcac
     1981 gcacttgtcc ggtgatacag agtttatctt cagatatgat taaagtggtg accttctcat
     2041 acgcataaaa aaaacctgaa actccgggga ggttcaggct ttgtagaatg atttattaac
     2101 gatcaggcgt tggtttcaat tttgattccg ccaacttcag tgatcttatt agggcaacct
     2161 tctttatgac cgcaggagct gagtccggct gtgaggagaa ggcataatcc ggctgcaaca
     2221 gcccattttg agaaggtacg catatgcttt aatgttttgt ttaacgataa cgattggttt
     2281 gcttaatcag ttcccacatt tgtatgtggt tacatactta ttataacgta aagatgacga
     2341 atatatttta cacaaacaag actgctttcc acatctccaa attcagcaat tagtatatct
     2401 tcaccccatg cgaatacatt tgatcgccat tggaggggca gtgatgcaca atatggcgtt
     2461 ggcattgcat gcggtaggtc atcaggttag tggttccgac gacgagattt tcgagcccag
     2521 caagtcgcga ctttcacgga aaggcttgtt accggaggct tttggctggt tcccggagcg
     2581 cataacaagt gaattggatg cagtgatttt gggtatgcat gcgcgagccg ataatcctga
     2641 gttattgcgt gcgcaggaat tgggcatacg ggtgtataat ttcccggaat atgtttatga
     2701 acaatcaaaa aacagtttgc gtgtagctat ttgtggaagt catggtaaga cctctataac
     2761 gagtatgatc atgcatgtgc tgaaaaaaag gcggatgcca ttcgattttc tggtaggcgc
     2821 tcagcttgaa ggatttgata ccatggtgca attttcacag gcaccggtga cgattataga
     2881 aggcgatgaa tattttgctt caccacttga caagcgtccg aaatttttat tttatcatgc
     2941 acaggtaaaa gtaatcagcg gtattgcatg ggatcatttc aatgtatttc ccactttcga
     3001 taactatgtt gatcaattcc gacaattagt aaatcaatca accgaaaatg atattttaat
     3061 tgccaatgca gacgacgaag aggtaataac attactggat atcagtcgcc caaaaggcaa
     3121 gatcatcaaa tatggcgccc ctgcatataa agtaattgaa ggttcattta cgcttgatgt
     3181 ggatggcgca cattatcaat ttgccatatt cggaaggcac aatatgcaga atatggaagc
     3241 ggcgaaaaat gtatgtatat cgctaggcat ttctgcacat gaattttatg aagccatgca
     3301 atcgtttaca ggtgcagcga agcgattaga agtattaaag cgcagcagcg atcatattat
     3361 tttcagagat tttgcgcatg caccaagcaa ggtaaaagct actgttgatg cggtacgcga
     3421 gcaattcccc gatcagcctt tagtggcatg tctggaatta catacctttt cgagtttgaa
     3481 taaagatttc atcgcacatt atcatggaac catgcaggct gccgacaaac gaattgtgta
     3541 tttcaatccg catactctta ccttaaaaaa actgccgatg ttgagtattg aagaggtcag
     3601 aagttttttt ggggataaag aattgattgt tttgaatgat tcctcgtcat taaaaagtga
     3661 attacaatcg ttaaatagtg tgaataataa tattctgtta atgtcgtcgg gcaattacga
     3721 caacctcgat cttacatttt taacctaatt ttgagcgtgg cacaactgca actggacaaa
     3781 ccccttgcct ttattgatct ggagaccacc ggcatcaatc ttggcagcga tcgcattgta
     3841 gagatcagca tactaaaatt gtttccgaat gggaacaggg aaaccaagac gatgcgcata
     3901 aatccgggga tacccattcc tgcggagtca agtgccgttc atggcatcta tgatgccgat
     3961 gttgccgaca agcccacgtt tgctcaggtt gctgacgagt tgaatgccta tctcgacaac
     4021 tgtgatttag caggatataa cagtaacaag tttgatgtgc ccatgttggt agaagagttt
     4081 cttcgctgcg gaaagggttt tgatgaaaat cgcaggtata ttgatgtgat gcgcattttc
     4141 acgctgatgg aaaagcgcac cctggaggct gcctatcgct ttttctgtga caagacctta
     4201 gagaatgccc acagtgccga agcagatgtt accgctacct acgaggtatt gctgggtcag
     4261 ctcgataaat atggcgatgc gcttgctcat gatgtccctt ccttgcatga gttcacgaag
     4321 gatggcgatt ttgtggattt tggtcgcagg atgatcatga aaaacggcgt gccatgtttt
     4381 aattttggta agtacaatgg tcagtcgtgt gaagacgttt atcgacgtga accgcagtat
     4441 ttcgattgga tatataaaag tgattttcct gaacatacca aactcaagct gcggctgata
     4501 gcattaaaaa ttaaaaacga caaaaacaaa gcttcctgat cccttggcac gcgcacttgt
     4561 aattatacca acttacaacg agcaggcaaa tatcgccgat atgataacgg ctgtattgtc
     4621 tttgccgcag gattttgatt tgttaatcat cgatgataat tcacctgatg gaacggcatc
     4681 agcagtgaca gcgatgcaat caacctaccc tacccgactg cacttacatc aacgcgccgg
     4741 caagttagga ttaggcactg cttatatcgc cggattcaaa tggggcttag cgcaaggcta
     4801 tgattatatg atagaaatgg atgccgattt ttcacataag cccgaagatc ttcccaggtt
     4861 attggacgcc tgtaaaaatg gtgccgatgt ggcaataggt tcacgttata ttaaaggtgg
     4921 caatgtagaa gactggccaa ccgatcgcat tttattatcg aaatgggctt cgcgttatgt
     4981 gcgcatgata acatggatac ctgtgcatga tacaacagcc ggttttgttt gttacaaacg
     5041 caatgtactg gataccatag atctggataa aatcagattt attggctatg catttcagat
     5101 agaattaaaa tacgctgcat ggcgcagtgg attctcattg aaagaaatac ccattgtgtt
     5161 taaagaccgc cagaagggga tttcaaaaat gagcaaaggc attttcaagg aagccgttta
     5221 tggtgtaatc accatgcgat ggaagagctt ctttcattct tataccaaat aagatttcat
     5281 gtcatcggaa caacgccata agcgaaaagg cattcataga aaaaaatcgg gattaccacc
     5341 gggcacgcct atttataccg gtgcgaaaaa gcatgaaata attacattac agcgttttgc
     5401 ctatgatact gatacaatag aagaaattaa ttgtaaggca ggtgataaga ttgatctaat
     5461 acccagagaa ggaaaagtat tatgggttaa tattaatggt cttcatcagg aagcgctgat
     5521 agaagaaatt gccgctacat tcaagttgca caatctggta ttagaagata ttttaaatgt
     5581 attccagata cctaaagcgg aagaatatcc cgatacagat cagatttaca ttgcgttaaa
     5641 cgaattttat tttgctgctg aaggcaaatt ggaacgcgat cagataagca tcattttagg
     5701 caatgattat gtactcagtt ttcaagaaga agagggtgat tattttgaat tgattcgcga
     5761 caggttgcgc aatgctaaag gcaaactgag aaagaaatcc ggcgattatc ttgtttacgt
     5821 tttaattgat tccgtagtag atagttatta tgttgtatta gatcattatg caacggaact
     5881 agacagtata gagaatgaca ttttcgaaaa gaaaaatcgc aatcacttag aacgtattca
     5941 tcaactcaac aaagacttga tttatttgcg cagatcaatt gcacctttga aggaagtatt
     6001 atttaaactc ttgaaggagg atgtaattct catcaatcct gagtcaaagc tgtttttgag
     6061 agatgtgctt gaccatgtgc atcaggtagt gaatcaaatc gatgtagacc gagagtatct
     6121 ggcagatctg gtgcagacaa atatggcgaa tatgaatgcc catttgaacg agatcatcaa
     6181 ggttttgacg ctcatttcaa cgattttcat acccttaacc ttcatagttg gtgtttatgg
     6241 catgaacttt ggaaatatcc cattaatgca gaccacttac ggctattttg tcgtatgggg
     6301 tgttatgatt ttcattaccc tgctgcagtt tttcattttc aaaagaaaaa agtggctata
     6361 agcatcatcc aatcacacaa aaaaacagct tatgcgttta acattaccca ttttcatttc
     6421 agcctgtatt ttgacatcaa acttgcaagc gcagagtacc attgcaccac cagccaacac
     6481 ctcggttctt tttaatatta atttcgatct tggcattccg atgaatgaat tcaaaaccta
     6541 caacgatgaa gttgctgcag gtggcggatt cagtttgttc tttcaaccgt caaataaaat
     6601 acctgttttg gtaggctttg atatggcatt tatgggcaac ggacataaaa cacagcatga
     6661 gacacttacc gcagatattg tggcaggtac aactgtgata gaaacgcttt atttccccat
     6721 gcgtgttgaa acctataata tgatcacaac cggtgatgtg catttgcgtg tgcaatcgcc
     6781 aaccaatttc tttaagccat atttggatgg tatggttgga tttaatcact tcggaacgaa
     6841 cacctctgtt tatgatgagt ctgaggaata ttatttgagt gaagaggaca atccattgat
     6901 caccagttct cagcaaaatt caagttggtc gctgaactac ggtggcgcct taggtatgtt
     6961 ggtgcaatta aatgaaaaca ttttccttga tgtgcgttgt gcctatacat ttggcggcga
     7021 agctgaatat tatgtggaag atgacattga aaactgggaa attgagttta atactgttcc
     7081 gacaagtcag gatgatgtat ctgatgaaga tatagatatt tcagccatca aaaagcaatc
     7141 gcatacagat atgatcatgg gtaccattgg actaaccttt aagttttaat cacattatat
     7201 accaatatac aacgctatcc taaatcgata gcgttttttt tgtttatata tgattatgta
     7261 aattcactac ctttcgcatc tatacaaaga acacaaataa ctacgttgat agcatatgcg
     7321 gtccggacgc aggaagttat cgcacaaggt gcatctggtt tacggcttgg tggtgatcat
     7381 agctgtgggc attgcatttt ttattctgca caacctaaaa gcatcaaacg ccagtcaatt
     7441 aaacgattcc tctgcaaccc cggtcatcga ttcgttgcat catattccgg tgtattataa
     7501 tggtgatgtt gatcatgttg caggtcgcag tgttgcgcgc aatggatata actacgggtt
     7561 aaaatggcaa tgcgtagaat ttgttaaacg atattatgca gatcatctgg gacactatat
     7621 gcccgatgct gatggtaatg cgaaggacct ttttgacaag cgtctgcctg atgcctcatt
     7681 taatgaagca cgtgcattag tgcaatatgc caatccaagt cttaaaaaac cgcaaccgga
     7741 tgacataatt gtttttgatg gccatttagg caatcgctac ggacatgtgg caataattag
     7801 tcaggttact gaaacaagta ttgaaattat acaacagaat gccggccctg acacaccaac
     7861 acgcgagtgg ctttcattag aaaaagttga caatcgatgg gaagtagatc acaagcatgt
     7921 attgggatgg ctgagggttg aataaaaaaa acaccgcagg tttctcaggc ctgcggtgcg
     7981 aaagcaatac tttgcttcgg tctatgaatg cgcttatttg tctgaatcat catcgtcgaa
     8041 attcatcgac tgcaccaact gcggaatatc ttctactttc agacggcaat gcattttcag
     8101 gtaaacaaat gtttcttctt tttcatcaac caatagcacc attcgtttta ttttatcatg
     8161 cttatttgaa cgaatagaaa tattcacggt ttcctcatca gtccgcacgc tcataagttg
     8221 ttcataatcg cggcgttcca tgcgatcaag tttgcgtgtg atttttttat cggtgcgatc
     8281 ggcataagct tctccttcgc ggatgcaaat atttatactt ccgaatttgc gtagcagcgc
     8341 gccggtggca gcaccttcag catccatatg atcttcaaca atatcggcgg ctatgcgcat
     8401 taccacaccg ggaatccaaa ggtttaattt tgttgattca tcttcaggtt caggtaaaga
     8461 agcggcatga gcggtattaa atccgactgc agcgcaaaat acgagggtga tcagcttttt
     8521 cattgtaata ggttgatgcg gtgataatta tttagtttct tctactttct tgagttcatc
     8581 aagaccttcg atatcgatat cggagatttg agaaattttg ctcagatcga tagcaccgag
     8641 tatgctgatc agtacaaatt caccatcggc atcgcaaacc atcaacatat cgttgagcgt
     8701 gctaccggtg atatttttac catacaatct caccttttct ccttctgcat ttacgttcat
     8761 caattcttca taaccatttg ttcctacggt ggtcataaat tcgttgtaat aagcgccgga
     8821 atttgaagca ccttctgcat tttcatatac aagaatgcga attcctttaa taccatcgat
     8881 catatttttc agatcagcat catcggtagt tgcagaagca acaagagaga ataatttttc
     8941 agtaacgatt acagaagtaa atccctcttt tccttcatat tttttaaaga attgatctat
     9001 ctgtgcagat tgagcgaaag ccattgcaga aatcatcaat gcgcaggcgg taaatagcat
     9061 ctttttcatt tgtagccgtt taggtggtga ttaaataatt ttgaacatta ggcgaatgag
     9121 gttcgcaaaa ggttacagct cgggcttaaa aaaaatgaaa taaatcgtca gaatgcttgc
     9181 ctatcaagcc ttacggctga taaaaattac caggagcaac atctggttgt agaggttatt
     9241 gagccttacg gaaaggttta ccggggctgt ttcgaggttg ctataaagtt ttggaaggtt
     9301 ttttaaatcc ctgattttcc cttctatcgc atacataaaa cgctcaacgt tccaacctat
     9361 atatttctct tgttgcttgg aaagaataac tgcagcgggc aatttttttg gtccttcaca
     9421 aagtgtgtca aatttataat tagcactata agcttcagca atagcagaag cagacaatcc
     9481 atcgatcatc gccattacat ttacacctga tttatcgcga tgttcgagca atttcatatt
     9541 gtcgcgcaac tcacgaagca gtgcattttt aattctgtcg cgcgatgttg atctgtcggc
     9601 aagaaactga aataattcct ttactgtagt tattccggtt tgcaggtagt cggccatggc
     9661 gatgaaggta caacagaaaa ttcaattagc cgtgattaca cgtcttattt cgaggaagtg
     9721 tttattatag tatttctcgg ttgcttcatt actaatgcgc actccaccgg tttcaggaga
     9781 tgaagtatgt ataaatgaaa agccgttttc tgcatcatag tccagcacga tacctacatg
     9841 tccgggatct ccgggaacaa ctgttgcact gctgaaaacg atgatatctc ccgggcgtgc
     9901 atcaacgtaa ggaattggaa tgccggcatc gaattgcata tcgctggtgc ggggcaaggt
     9961 gatattaaaa gccttgtata catagcaaat aaatcccgaa caatcgaagc catgttgagg
    10021 atctgtgcca ccccatacat aaggagtgcc gataaatgtt ttggcataag caataatgct
    10081 atcggcttta gccagctctt taatttcagt agaatctacc tgctgttctt gccaccaggg
    10141 agtgagttgg gcatgcattt ttgcactaaa aaataccatg catacacaac acactatgcg
    10201 ggcaagatta ttcatcaggg atgctggctt ttctttttct tcttcttttt agcatcgcgc
    10261 tcccaccata caggagtttg atcttccggg tcgagattat aatttacggt aatctcttca
    10321 ccgggttcga tatcgcgcac tgcctggaat tcgagtacgt tgagatcgaa atcagggata
    10381 tatcgcgcat ttggagaata gctatgattg tataatgaac caaatcctaa agcgagagca
    10441 cctttttttc ccttcttccc ccactcgaaa taatattcgt aaaggaaagt atcgtcaata
    10501 ctcaagcgat ctttttcgct gaatactatc attggcgcca cttccagggt atcgccagct
    10561 tttacggctt ttgaggtaaa cacggcacgt cctttatcgg gagtgcgtgc cacaaataaa
    10621 tgatcaggca acacgggtgt gagtttcctc actaccactt cttctttttt ttcttttttc
    10681 gacatcacct gattatttag cggaaactac atcctccaac tgtttcttca catcggcaaa
    10741 aagggcagta aaatgttttt ttacagcact ttttcctttc accttgctgc tgccattgat
    10801 ttttttcacg gcagcttcat atgtattaaa aagtgcattt attttagatt ccaattgttc
    10861 atcggtgata tccatgaaca acacacggtc gatcattgat gcaacggcag cgccgattct
    10921 tttcttagca ttacgtttac tcatgtcaat attttcagca aagttaagca tatggctatc
    10981 tttgcgcaat aaataccttg tctatgtttg atatcaagaa ggctatggag atgaaaaagc
    11041 agatggaaga gatccagaag cgattggata acatcactgt ggagggcgtt tcgggtgatg
    11101 gaaaatttca ggttgttgtt acgatcaccg cgaataaaaa ggtcaaatct gtgcatatca
    11161 gcgatgaatt gatggcatcg ggcgacaaag aacacctcga ggacctgctt gtcatagcta
    11221 tggagcgcgg tttagacaag gcgacacagg tatcggaggc tgaagctcgg aatgtggctt
    11281 tatcgggagg gttgaacggt ttattcgggt cctgatcaga cgagttcttc tactttgaag
    11341 atggcgcttt ttacacgttt accggcgtca tcagacaata tcagctcttt cgcagacggt
    11401 agttcaaaca cctgttgtaa gtccctgata gcacctaccg gaacatgatt ttgacggcat
    11461 aattcgagga aactatcgct tgaatatgac tgcactttgg attgcagcac tcctatcaac
    11521 gtatttctgt tgtgcacgcg atcggtatta tgcgcatagc gttggtcggc atatacctca
    11581 ggacagttaa gaatatcgca taaatttttg aattggcggt cggaaccgat tgccagcacc
    11641 acctgcacgc catctttaca agtgagaatc tcgccatagg gagcgatatt aggatgcaga
    11701 gaaccattgc gttgcgggat atggccggca gtgatataat tcgcagcctg attggcaagc
    11761 gccgcgatgg aagcatcaaa tagcgataca ctcacgcgac ttccttttcc tgttttatac
    11821 ttttgaatga gggctgtcag gatgccttcc tttaattgat gggaagcaaa taaatcgata
    11881 atagctacgg gaagtttaac gggaggtgag ctaggggtgc cattcatgaa catccaaccg
    11941 gtttctgcct gcaggacaac atcaaaagca acgcgatcct cttcatcgcc aaaaccgttg
    12001 atattggcat aaataattcc ggggttcagc gccgccacgg tgttaggatc tagcttgaat
    12061 ttctgtgcat ctcccttttt ccagttgcat atcatgacat cagcctgtct gatcatatca
    12121 taaacctgct ccagacatga agggtcttca aggtcgagga agaggtggag tttgcgccaa
    12181 ttgactgctg cgaaataagc tgaggttgag gcggcaggat tttcgcttgg aagcttccac
    12241 tgtctggtga tatcgccacc ggtgcgagga ttttcaattt tcactacatc agcacccaat
    12301 tccgaaaaaa atgaacccgc agcaggcccg gccattacac cggccaattc aataatcctc
    12361 aatcctttaa acatgcatca aaattacaag gaaataacaa tgttaacttt ttataaataa
    12421 cgataaaagt aaggcaagcg ttggcagcaa agtcgaagaa aaggccgatt ttcttacata
    12481 ttcgactgct aatcatggtt ttagaggtaa aaatgtccgg tttttagtgg tttatttcga
    12541 tatccttaca atattaagtc tttgccggcg tcagaaccaa acagtaaaaa tgacgttaac
    12601 attaccatat gctaaggcga ttattaccta ttatcatttt tgtcatcggc gctgacatag
    12661 tatctattca ggccgctacg aatatttttg gatcctctac tctactttat ggcatttgga
    12721 ccggtgcaac agttttggtt ttggccagct tgtttttgta tttcagtcgc gaaatgcgca
    12781 aacgaagcgg tatagcgaga cctgttttga tgagtacttt tttcatgcta ttgtttccaa
    12841 aagttattgt ttccgtcctg ttattgatag aggatgttgt aaggctgaca aagctgatgg
    12901 caaatggtgt tattactgct cttggatcgg attttaattt cggattaggg aggtcatttt
    12961 ggtgggatgc atttgccctt tcatttgcgt tattattgac cttagcattt gtttatggag
    13021 cgatattcaa tgtgtatcgc tatcaaattc gaaaagtaaa agtgaaactg gtgaaattgc
    13081 caaaagcgtt tgatggcttg cgtgttgtgc aaatttctga tatacattcg ggtagtttaa
    13141 gtaatcgaga ggcagttgca aaagctgttg acaggataaa ctctttatcg ccggatttga
    13201 tttttttcac gggtgatctg gtaaataata ttgcggagga ggcgcgcgga tttgtagaca
    13261 tattcgccaa gttgcaggca aagcatggcg tttacagtat tcttggcaat catgattacg
    13321 gcgattatat ggcatggccg gatgaagcta ccaagcagca taatttagaa acgctcaaaa
    13381 ccatgcatgc gcaaatgggt tggcgattgc tcttaaatga acacgtgcat attgaacgat
    13441 ctggaaataa aattgcggta gcaggagtag aaaactggag tgccagaggt aggtttaaaa
    13501 gttatggcag attagaacag gcattgcatg gcattcagga tgccatgacg gtattattgt
    13561 tatcgcatga tccttcgcat tgggagggtg aagtattaga ccatcctgcc aaagtggata
    13621 taaccttttc gggtcat
//
LOCUS       DAOQOU010000083        13632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2327_length_13632_cov_5.182809, whole genome shotgun sequence.
ACCESSION   DAOQOU010000083 DAOQOU010000000
VERSION     DAOQOU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_13632_cov_5.182809"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1332)
                     /locus_tag="PLM90_08485"
     CDS             complement(289..1332)
                     /locus_tag="PLM90_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ89449.1"
                     /translation="MKSNVYIFNLNLDWELIKLISQIDRFDAAWTAIERREQQGLKEL
                     KSIATVRSVGASTRIEGSKLSDEEVDVLLRNIDISKLEDRDTQEVVGYFEALDIISEQ
                     FEEIKITESDIKNLHNILMKYSAKDEWHKGNYKQHSNAVEARYTDGTRQILFQTTEPG
                     VETETAMRLLVNWYNNEHAIHPLVKCAVFTYEFLSIHPFQDGNGRLSRLLSSLLLLKS
                     GYKWIQYISFEHEIESRKHAYYQVLRSCQTLRPNIDISTWVNFFFDALKNIQELLERK
                     LLIYETETDQLLPREKLVVSFIQNHPGCKSGQISKGLGFSRMIVIRLLSSLLEKKLIE
                     KHGTGSGTQYTIM"
     gene            1588..2118
                     /locus_tag="PLM90_08490"
     CDS             1588..2118
                     /locus_tag="PLM90_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89450.1"
                     /translation="MKPKLTLLLLIPLLAMSFLIKNDPQTNEINLNTLDQQLIGEWEY
                     KISFSDSSYFTVPFNRILPVQKLRFEHCHDSSELERFPKIVIAMRKHNSLENISCKAY
                     NENNEVIDTYYPVVLFGNDSLVNQIDVSQYGHILKSEYTIYTLTSDSLIIGDGKLFES
                     PDRKMVLVRHIYLRKN"
     gene            2233..2616
                     /locus_tag="PLM90_08495"
     CDS             2233..2616
                     /locus_tag="PLM90_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89451.1"
                     /translation="MSRLILSLIATVTFILLTSMSACMKYDIERRANPSNIEVYDSIY
                     LKNGNYCFWYAYNLGGIYGGIGDYISIAPQRSQISDSTQVIVSEYIKGIRAISENEIV
                     VILTEDEYDEIHTTFKGIKISAKID"
     gene            complement(2741..3553)
                     /locus_tag="PLM90_08500"
     CDS             complement(2741..3553)
                     /locus_tag="PLM90_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HMZ89452.1"
                     /translation="MVKPQVNAASVTTKRKEIAIFKVPIFRAFLQLIDGVDYICDMCS
                     SYTTKSKMVKEVYEQSFGRPWAEEDLGIEEKYHDESGNNAIAFPGTPMPVVTIERPQI
                     IQAYQFGFIPHWTAPEKVNEQKATFNARIETITELATWRDAWKHGQRCLVCTNGFFEH
                     NKAEKRKMFIHLQDAENFFYAGLYNNYINKKTGEIIRTMAVITTTPNSLIASIHNRMP
                     VIIPIGTESPWLDPNADLKSLLANYTTPFSPAHMLMEYADKKPQKPVQGELF"
     gene            3546..4388
                     /locus_tag="PLM90_08505"
     CDS             3546..4388
                     /locus_tag="PLM90_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ89453.1"
                     /translation="MTIGGAGCTMDSDTTTQNIPQPEHVVIVMFENKDFAQVYDTSIA
                     PFLQTLIYDPHTAVFTNSLAMGHPSQPNYIWWFSGDDQGVTTNDRPATKFNTPNLAAE
                     LIQNGFSFVTYSENLPFVGFDGDSSENGLYVRRHNPVSNWIGLDVNQVSPDVSQPFSA
                     FPANFDHLPTVSFVVPNMINGMHNGTITEGDTWLQNSLSEYIEYCRNNPSLLVVTFDE
                     TTYNVTDNRILTLCFGSLVNGGEYDAAVSHVNFLRTFEDMFSLPHAGLSGYLEPISNC
                     WVQE"
     gene            4391..5296
                     /locus_tag="PLM90_08510"
     CDS             4391..5296
                     /locus_tag="PLM90_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ89454.1"
                     /translation="MRCKKLFIGLVTLTLSWFPSTVFSQDTIRAHIYFKSGSYETKPH
                     NTYLKRFADACAGFDSAHIFIDGFCDDEGSDAFNIQLSEQRAMAVEAFLKQYITIKAN
                     FTITGHGESNPVASNEFDDTKFQNRRVDMLVIGYQYGYTSSISDISQIVDAVPEFVEL
                     SDVSLRELLLALEKDDRLVLRDVQFPMSSDSILPSSFIVLDTLASILMDYPNLIVQLE
                     GHICCTSETGMAGHKDAYNATNGKYSLSSDRALNVFMYLVYKGVPSRQLKYVGLSGSQ
                     KLVWPEATAKDQQKNRRVEVRILEK"
     gene            5445..7943
                     /locus_tag="PLM90_08515"
     CDS             5445..7943
                     /locus_tag="PLM90_08515"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HMZ89455.1"
                     /translation="MFKATLFSAISATIFSFGNELDAQDSIPKPSMPSTKVTIAPKID
                     GNLQDEVWQSVQPVTLDVTFLPEFGKTPSRKTEVRVVYDNNAIYVGAIMYDPDPATIN
                     RQLCERDGYSNADNFVFGLDTYNDDLNGYRFTVSAAGVQSDQRIGLNSNGDMSWDAVW
                     ESDVSINELGWVCEIKIPYSAVRFPSKPLQDWGMQFNRNVNSTGELILWSPVDPKVYG
                     IINQWGTYTGMENIIPPLRLSFSPYLTAGNQWTPDPENEGGTVSEGILNGGLDVKYGI
                     NESFTIDATLIPNFGEVQSDNVVLNLSPFEQQFNERRPFFTEGTEIFNQTFNFLSDRM
                     FYSRRIGGTPIFYYDVYNETGAHEIIKENPFETSLINATKFSGRTDKNVGIGVFNAIS
                     AQEFAIIEDTVTGETRTVLTNPMTNYNMLVFEKALKNNSKVSFTNTNTLREGIYRDAN
                     VSQALYDITNKKHTWNFWGFGNYSQVFEPYYSGESEVVEETKDGYNYIVGISQFTGKW
                     NFNFSHALISPEYDHTDMGIQYGYNFINNYIGFNYNNQEPKKGVFYNYSGWGGFTYNL
                     QAEPLEYQEVNYNLGFNGQFKNFWNIGGNLYSKPLWWYDFYEPRVEGRKYYHAPFTFI
                     NVWGGTDYRKDISFNWNISFGESPIPNDPYIGGGQWITWNISDKFSLTHGISLSKDHS
                     NFGFVDFDRGGNSIFGRRDITNIDHEIGMKYLFGPKMNIIFRAREYWGKVVYHQFYTL
                     LDDGNLGDTDFTGNYDLNFNIFNLDFVYSWEFAPGSYLNVIWKNNIFQADDIRYDDYF
                     DNLNKTFNAPQSNGLSLKVIYYLDYQYLHKNKQS"
     gene            8034..8429
                     /locus_tag="PLM90_08520"
     CDS             8034..8429
                     /locus_tag="PLM90_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89456.1"
                     /translation="MKKTLFLLSVAASVILVTSCESNTHWDKTFVNSSTHTLILYTTD
                     GGGGFTFADSIILAPGAEEVIYSFSDVVNDAAPACEQYINRLSLSAEEGFTITKDVSD
                     SDNWIDDSVEESHGFMHNCTFTISDSDIL"
     gene            8467..9792
                     /gene="chrA"
                     /locus_tag="PLM90_08525"
     CDS             8467..9792
                     /gene="chrA"
                     /locus_tag="PLM90_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HMZ89457.1"
                     /translation="METPEKPSFKEALTFWFKLGWISFGGPAGQIAIMHTYLVEQKRW
                     ISEKKFLHALNYCMLLPGPEAQQLATYTGWLLHGKRGGLAAGILFVLPSAFILLILSI
                     LYVQFGHIAEVNALFEGLKPAVVAIVALAMVKIGKRSLKYALHVAIAILSFVAIYFFN
                     FPFPAIIGLILLFGLLALWLKPSLLHPSGQQSGTDDDESGYFLTTATVLPHTGFEPLR
                     LVKQIGVFGMAWLLPFILLTNIGDRELWSNVVLFFSQAAFVTFGGAYAVLPYVSQQAV
                     EHYQWLGHLQMIDGLALGETTPGPLIMVLAFVGFMAGYGYSGSLAMGTLGLFATTYYT
                     FLPCFLFIFAGAPLIEKTQQNPNIKAVLGLVTAAVTGVILNLTLYFAEAVVYGKHITT
                     WSDIRPENIHPFALIWIVISLIAMGRFKIGMITWIGISAIFGLITYLLA"
     gene            9827..12298
                     /locus_tag="PLM90_08530"
     CDS             9827..12298
                     /locus_tag="PLM90_08530"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMZ89458.1"
                     /translation="MRYFKIFIFILIPISLIFVLDKGVAGLPPLGKLMDPVRGFMANA
                     ETSSSYTDVEIPAEEHHVSGTVYLDERLVPHIFAADDRSLYFIQGYVTAKYRLWQMDI
                     QSRAAGGRLSEIFGERFIPYDLEQRRMGMVYSAEKAVELWKKDTVMYANILAYTEGIN
                     AFIDPLLEEGYEAYPIEFKLLDYKPEEWTPLNSALLLQYMSSTLTGFDDYLEYSNLRK
                     LLTPEEFAALFPDRPEGIDPIIPSEKVYNFTVNDADSIPQDARTGYFKKVLEIFESQH
                     DHTQIGSNNWAVDSSRSKSGHPILANDPHLQLNLPSLWFEIQLQTPATNCYGVSLPGA
                     PAIVIGFNENISWGVTNAGIDVRDWYTIEYKDDTKSAYKYGKTWKKTEQRIETIDVRN
                     ADPIIDTVIYTHYGPIVYEDEEITAGTKQDPHKVQRIDLAMKWAALEPGEQIKTFFYL
                     NRAKNYNDYRQAIAYFNCPAQNFVFASHNGDIAITQQGKFAVKKKEQGRFVLNGSDPG
                     DDYTDFIPFDRNPGILNPMRGFCSSANQHPTDATYPYYYTGFDFEFYRNRIINRSLDA
                     MHDATIESMEKLQQNNYNLHASEALPLMLDKLTYDVGTPDSTAYTVKTALSEWNFMND
                     ADMLAPTYFQMWWDTLYAMMWDEMEDSVIAYVRPNKYNTVKAMHAFPNDQRLFDMIST
                     PDQKETLDELINLSYGIMVHMVDSLAAEDKNLLQWYRHKRTNIMHISQIPAFSKMSVH
                     NGGYQSIVNATSETHGPSWRMIVEMSNPVKAVGVYPGGQSGNPGSKYYDNFVDDWAAG
                     RYYELHLYQSEEEAKEQAQFTMRFN"
     gene            12300..12644
                     /locus_tag="PLM90_08535"
     CDS             12300..12644
                     /locus_tag="PLM90_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89459.1"
                     /translation="MKYFVSITIGFTLCMAGQFFLPWWWIFAPLTFLISFIVNYHSAW
                     RAFFAGLLIVFISWMAAYIYKDLANDSIMSGKMANLFNFKNNYALFFIVAIAMGIIGG
                     LTSTAAYFTRNS"
     gene            12648..13550
                     /locus_tag="PLM90_08540"
     CDS             12648..13550
                     /locus_tag="PLM90_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89460.1"
                     /translation="MSDFDVLLITEDRYESPQHIDGYIANILQDDAILTAAFEEVGLR
                     TKRVSWSNPEIDWSASSCGLFRTTWDYFLRFNQFKNWMEQTAQKVIFINPIKVIRWNM
                     DKHYLADLKATGIRVVDTTFIEQNTSCDLVALLQASDADSGIVKPAVSGTARHTYVVD
                     NSSLESVQPIVNDLLKSEALLFQPFQKDILLNGELSLIIIDGKCTHAVRKTAKQGDFR
                     VQDDFGGTVHDHIPTPEEIQFAESAVAACPEKPLYARVDIVRNNAGDLAIMELELIEP
                     ELWFRKNPAAAKPLAAAIKNIYFS"
BASE COUNT         4054 a         2756 c         2757 g         4065 t
ORIGIN      
        1 gggagtgtgg aagtatgcta gtgtgcaagt atgcaagtaa atggtaacgt ttaattagcc
       61 aattagccga tttgggttcc ctctgcgcct ctgcgcctct gcgtgaaaaa ccctccacac
      121 cacaatcacc tgtcacgact cacccttgtt taatcgcgga gtttgcagag ttagcggagt
      181 ctcaccactc accactcacc actcaccatt caccattcac cgaaagtccc ctagtcggat
      241 atcactacgc ttcaatttca ggagtaatta atagtgaatc ttcccttctt acataatcgt
      301 atactgcgtg ccggaacccg taccgtgttt ttcaataagt tttttctcaa gcagggagga
      361 aagcaatcta attacaatca ttctggagaa acccaaccct tttgatattt gtccggattt
      421 gcaaccggga tgattttgaa tgaaacttac gactaatttt tcgcgtggga gcaattgatc
      481 tgtttcagtt tcataaatga gtagtttccg ctctaacaat tcctgaatat tttttaatgc
      541 gtcaaaaaag aaatttaccc aagtgcttat atctatattc ggacgcaagg tctgacagct
      601 tcgcagcact tgatagtaag catgttttct gctttcaatt tcgtgttcaa aactgatata
      661 ttgaatccat ttatatcctg actttaataa gagtaaagat gaaagtaatc gactcaatct
      721 tccgttccca tcctgaaatg gatgtatact caaaaattca taagtaaaaa cagcacattt
      781 aaccagaggg tgaatcgcgt gttcattgtt gtaccaattc actaagagcc tcattgcagt
      841 ttcagtttca acacccggtt cagttgtttg aaaaagaatt tgcctggttc catcagtgta
      901 cctggcctca acagcattgc tatgttgctt gtagttgcct ttatgccatt catccttggc
      961 actgtacttc atcaaaatat tatgtaaatt tttgatgtcg ctttctgtaa tttttatttc
     1021 ttcaaattgt tcggaaatga tatctaaagc ttcaaaataa ccaactacct cctgggtgtc
     1081 tctgtcttca agcttggaga tatcgatatt tctcaataaa acatctacct cttcatcgct
     1141 caatttagac ccttcaattc tggttgaagc accaacactt ctaactgtag caattgattt
     1201 aagctctttt aaaccctgtt gctctctcct ttcaatggcc gtccaggcag catcaaatct
     1261 gtcgatctga ctaatcaatt taatcagctc ccagtctaaa ttgagattga atatgtatac
     1321 gttactcttc attattcaaa ttaaaatgat atgaaatgat acaatatgat acaaagttat
     1381 acaatatgat acaaataatt gcaaaaagtt acgatttcat tcaaattcaa taaaagatca
     1441 ggataatctt agatatatgt cattgattaa caattaatta gttgcgataa acgggattta
     1501 tatagaaaac aaaatagtcc taatgaatat agatggaaca ggtgaaatga ttatcttaga
     1561 ataattaact tcctatttag gcccccaatg aaaccgaaac tcacactatt gcttttgatt
     1621 ccacttttgg ctatgtcgtt tttaattaaa aatgatccac aaactaacga gatcaatttg
     1681 aacacattag accaacaact gattggtgaa tgggaataca agatatcctt ttcagattca
     1741 agttatttta ctgtgccatt taatagaatc ctaccggtac aaaagttgag atttgagcat
     1801 tgccatgatt cttctgaatt agaacggttt cccaaaattg tgattgctat gaggaaacac
     1861 aattccttgg aaaacatttc ttgcaaagca tacaacgaaa acaatgaggt gattgacacc
     1921 tactaccccg ttgtattgtt tggtaacgat tctctggtaa atcagattga tgtcagccaa
     1981 tacggacata tcctaaagtc tgaatataca atttatacat taacttcaga tagcctaatt
     2041 attggggatg ggaaattgtt tgaatctcca gataggaaaa tggtacttgt cagacatatt
     2101 tatttaagaa aaaattaaat attatgtcgt ggtttcattt taaactgtaa aagaatatta
     2161 tatttgaaat atcaataata taaatgcagg atccatggct ttaatagaag aagaaaatca
     2221 cacccttaaa tcatgagccg actaatcctc tcgctaattg ccactgtcac ttttatctta
     2281 ttgacttcaa tgagtgcttg catgaaatac gacatagaac gtagggcaaa tccttcaaat
     2341 attgaagttt atgattcaat ctatttaaag aacgggaatt attgcttttg gtatgcctat
     2401 aacctaggag gaatttatgg aggaatcggc gattacattt ctatagcacc ccaaagatct
     2461 caaatatccg actctactca agtcatcgta tcagaatata tcaaaggtat tagggccatc
     2521 agtgaaaacg aaatagttgt aatcttgaca gaggacgaat atgatgaaat acataccaca
     2581 tttaaaggaa ttaaaataag tgcaaaaatt gattaaaata taaacagttt gcgtgtaaac
     2641 aaatgcatca acttatgaaa ttgataaaca atcccataac aaaccttttg caatttctcg
     2701 aataaactaa aatcattagc ggattgtttc aacttcacac tcaaaacaac tccccctgca
     2761 caggcttttg tggtttttta tcggcgtatt ccatgagcat atgggcggga ctgaagggtg
     2821 ttgtgtaatt agccagtaga ctttttaaat cggcattcgg atctaaccag ggcgattcgg
     2881 ttcctatggg aataataaca ggcatgcggt tatggatact cgcaatcaaa ctattcggcg
     2941 tggtggtgat tactgccatc gtgcgaatga tctcgccggt ttttttattg atgtagttat
     3001 tatataaccc tgcataaaag aaattttcag catcctgtaa atggatgaac atcttgcgtt
     3061 tctctgcctt attatgttcg aaaaacccat tggtgcatac gaggcaacgc tgaccatgct
     3121 tccatgcatc gcgccaggtt gctaactcgg taatggtttc aatacgcgca ttgaaagttg
     3181 ccttttgttc atttactttt tcaggtgctg tccaatgggg aataaatcca aattgatagg
     3241 cctgaataat ttgtggtcgc tctatcgtaa ccacaggcat cggcgtgccg ggaaatgcaa
     3301 tggcattatt accactttca tcgtgatatt tttcctctat gccaagatcc tcttctgccc
     3361 aaggtctgcc gaagctttgt tcataaacct ctttaaccat tttcgatttt gtggtgtagc
     3421 tcgaacacat gtcgcaaatg taatcaacac catcgatcag ttggagaaat gcccggaata
     3481 tcggaacttt gaagatggct atttctttgc gttttgttgt aacactagcg gcgttaacct
     3541 gcggtttgac cattggtgga gcaggatgca ccatggattc tgataccact acccaaaaca
     3601 ttccgcaacc ggaacatgtt gtcattgtga tgtttgaaaa caaagacttt gctcaggtgt
     3661 acgataccag cattgcaccc tttcttcaaa ctttgattta cgacccccac actgccgtat
     3721 ttacaaattc tcttgccatg ggtcacccca gccagcccaa ttacatctgg tggttttcag
     3781 gcgacgatca aggtgttacc acaaatgatc gtcccgcaac aaaattcaat accccaaacc
     3841 tggcagcaga attgattcaa aatggtttct ccttcgtcac ctacagcgaa aatttgccat
     3901 ttgttggttt tgatggcgac tcttctgaaa acggactgta tgttcgacga cacaaccctg
     3961 tttccaattg gataggcctt gatgtcaatc aagtttcacc tgatgtaagt cagccctttt
     4021 cagccttccc tgccaatttc gatcacctgc ctaccgttag ttttgttgtg ccgaacatga
     4081 tcaatggaat gcacaacggc actatcaccg aaggcgatac ctggctgcaa aatagcttat
     4141 cggaatacat tgaatattgt cgcaataatc cttctctttt ggttgtcact tttgatgaaa
     4201 caacttataa tgttaccgac aatcgcatac ttaccttgtg ctttggttcg ttagttaatg
     4261 gcggagaata tgatgcggct gtttcgcacg tgaattttct gcgcacattt gaagacatgt
     4321 tttcattacc acacgccgga ttatccggat acctggaacc aatcagcaat tgctgggtgc
     4381 aggaataatt atgcgctgta aaaaattatt tattggttta gtaacgctga ctttaagctg
     4441 gtttccgtcg acagttttta gtcaggatac catacgcgcc catatttatt tcaaatcggg
     4501 atcatacgaa acgaaaccgc acaacactta tctgaagcga tttgcagacg cttgcgcagg
     4561 atttgattct gcacatattt ttatcgacgg cttttgcgat gacgaaggaa gtgatgcctt
     4621 caacatacaa ctctcagaac aacgtgctat ggctgtggag gcttttttga aacaatatat
     4681 taccatcaaa gcaaatttta ccatcaccgg tcatggcgaa tcaaatccgg ttgcaagtaa
     4741 tgaatttgat gacaccaaat ttcaaaatcg cagggtggat atgcttgtca ttggttatca
     4801 gtacggttat acttcttcca taagtgatat ttcacaaatt gtcgatgctg tgcccgagtt
     4861 tgttgaatta tctgatgtat ccctgcgtga gttattgctt gccttagaaa aagatgatcg
     4921 gttggtgctg cgcgatgtgc aattccccat gtcgagtgat tcgattttac catcgtcatt
     4981 tattgttctg gatacacttg caagcatttt gatggattat cccaatttga tagtacaatt
     5041 ggaaggacat atctgttgca cctccgaaac cggtatggcc gggcataagg acgcctataa
     5101 tgctacgaat ggcaaataca gtttatcctc agacagagca ctgaacgtat tcatgtatct
     5161 ggtttacaag ggagtacctt cccgacaatt gaaatatgta ggactttcgg gttctcaaaa
     5221 attggtatgg cctgaggcta ctgcaaaaga ccagcagaaa aatcgacgcg tagaagtgcg
     5281 catactcgaa aaataatgcc cattttttac cacaacccct gtgtataaag cccttatgcc
     5341 tgttgattta acagcattta acccaacatt aaccgataga aatatcacat taaccttttt
     5401 tatcaaatgt tgcaagtttc tttgcaatta ttaaaaccca atacatgttt aaagccacac
     5461 ttttcagtgc catctccgcg acaatttttt catttggcaa cgaactggat gcacaagaca
     5521 gcatacctaa accgagtatg ccatccacaa aagttaccat cgcaccaaag atcgatggca
     5581 acctgcagga tgaagtgtgg cagtctgttc agccggtaac tctggatgtt accttcctac
     5641 cggaattcgg aaaaacaccg agcagaaaaa ctgaggtgag ggtagtttat gacaataatg
     5701 ccatttatgt tggtgcaatc atgtatgatc ctgatcctgc cactatcaat aggcaacttt
     5761 gtgagcgtga tggatattca aatgccgata attttgtttt tggtttagat acttacaatg
     5821 atgatctcaa cggctatcgc ttcactgtca gtgctgccgg tgttcaaagc gatcagcgca
     5881 ttggactgaa cagcaatggc gatatgagtt gggatgcagt atgggaaagt gatgttagca
     5941 tcaatgagct gggatgggta tgtgaaataa aaatccccta ctccgcagtc aggtttccgt
     6001 caaaaccgct gcaggactgg ggaatgcaat tcaaccgaaa tgtcaactca acaggagaat
     6061 taattttatg gagtcctgtt gaccctaaag tatatggtat tattaatcaa tggggtacct
     6121 ataccggaat ggaaaatatt attccaccat tgcgattgtc gttttcacca tatttaactg
     6181 caggaaatca atggacaccc gacccggaaa atgaaggtgg aacagtaagt gaaggcattt
     6241 taaacggcgg cttagatgta aaatacggta tcaacgaaag ttttaccatc gatgctacac
     6301 ttattccgaa tttcggcgaa gtgcagagcg ataatgtagt attaaactta tcgccgtttg
     6361 aacaacaatt caatgagcgc agaccatttt ttactgaagg aacagaaatt tttaatcaga
     6421 cctttaattt cctcagtgat cgcatgtttt attcgcgacg cattggcggc acacccatat
     6481 tttattacga tgtatataat gaaacaggag cacatgaaat cattaaagaa aatcctttcg
     6541 aaacaagtct tatcaatgcc acaaaattct ccggacgcac cgataaaaat gtaggtatcg
     6601 gcgtatttaa tgctatttct gctcaggagt ttgcaattat agaagatact gttaccggag
     6661 aaacaagaac agtgctgacc aatccaatga ccaactataa tatgctggtg tttgaaaagg
     6721 cattaaaaaa caatagcaag gtcagcttta ccaataccaa cacacttcgc gaaggcattt
     6781 atcgcgatgc caatgtttcg caagcgctgt atgacattac caacaaaaaa catacctgga
     6841 atttttgggg atttggaaat tacagtcagg tatttgagcc atattacagc ggtgaaagtg
     6901 aagtagtaga agaaaccaag gatggttata attacattgt cggcatttct caattcaccg
     6961 gaaaatggaa tttcaatttt tcacatgcct taattagtcc tgaatatgat catacggata
     7021 tggggattca atatggttat aatttcatta ataattatat cggatttaat tacaataatc
     7081 aggaaccgaa aaaaggtgtt ttttataatt atagcggctg gggcggattt acctataact
     7141 tacaagcaga acccctggaa tatcaggaag tgaattataa tcttggattc aacggtcagt
     7201 ttaaaaattt ctggaatatt ggcggaaact tatattctaa accattgtgg tggtatgatt
     7261 tttacgaacc acgtgtagaa ggaagaaaat attatcacgc acccttcacc tttatcaatg
     7321 tttggggtgg taccgattat cgcaaggata tttcttttaa ttggaatatc agtttcggag
     7381 agtctcctat acctaacgat ccatacattg gcggtggtca atggatcaca tggaacataa
     7441 gcgataaatt ctcgctgacg catggaataa gcttgagtaa agatcatagc aatttcggct
     7501 ttgttgattt cgatcgcgga ggtaacagta ttttcggaag aagagatatc acgaacattg
     7561 atcatgaaat tggtatgaaa tatttattcg gtccgaagat gaatatcatt ttcagagcaa
     7621 gagaatattg gggaaaagtg gtgtatcatc aattttatac tttgctcgat gatggtaatc
     7681 ttggcgatac cgatttcaca ggaaactatg acttgaattt caacatcttc aacctcgact
     7741 tcgtatattc ctgggagttt gctccgggaa gttatctgaa tgtaatttgg aagaataata
     7801 ttttccaggc cgacgatatc agatatgacg attattttga caatctgaat aaaacattta
     7861 acgcaccgca atcaaatggt ttatcactga aggtgatcta ctatctcgat tatcaatacc
     7921 tgcataaaaa caaacaatct tgatttcggt catggcagcc gcactttagt gacacaagtg
     7981 ttcacatatg tggttttgtg gtttcagggg cttggactat ctttccgatt gtcatgaaaa
     8041 agaccttatt cctcctgtcg gtagccgcct cagtgatcct ggttacctcc tgtgaatcga
     8101 atacacattg ggacaaaacc tttgtaaaca gcagcaccca tacgctgata ctgtatacca
     8161 cggatggagg cggaggcttt acctttgctg attctatcat tctggctccc ggagctgaag
     8221 aggtgattta cagctttagc gatgtagtta atgatgccgc tcccgcttgc gaacagtata
     8281 ttaaccgctt aagtctaagc gccgaagaag ggtttaccat caccaaagac gtttctgatt
     8341 ccgacaactg gattgacgat agtgttgagg aaagccacgg attcatgcat aactgcacat
     8401 ttacaatttc agactcggat atcctctgaa acctgcggaa acgaagcttc caaaggcatt
     8461 agaaacatgg aaacaccgga aaaaccaagt tttaaggaag cgcttacctt ttggtttaaa
     8521 ttaggttgga taagcttcgg tggtcctgcc ggacagattg ccatcatgca tacctatctg
     8581 gtagaacaaa agcgttggat tagcgagaaa aagttcctcc acgccctcaa ttactgtatg
     8641 cttcttcccg gaccggaagc acaacaattg gcaacgtata cgggctggtt gctgcatggc
     8701 aaaagaggag gtttagcagc cggcatactt tttgtattgc catcagcatt tatcttattg
     8761 attctcagca ttttatatgt tcaatttggc catattgctg aggtaaacgc cctttttgaa
     8821 ggactaaaac cggcagtggt ggcaatcgtt gcacttgcaa tggtgaagat cgggaaacgg
     8881 tcgctcaaat atgccttaca cgttgcaata gcaattttaa gttttgtagc tatttacttt
     8941 ttcaactttc catttccggc cataatcggc cttattttgc tgtttggctt actggctttg
     9001 tggttgaaac catcgctctt gcacccttcc ggacagcagt cgggaacaga tgatgatgaa
     9061 tccggatatt tcctcaccac agccacagta cttccccata ccggcttcga acctttgagg
     9121 ctggtaaagc aaattggtgt ttttggcatg gcatggctat tgccgtttat tttgttgacg
     9181 aacataggcg accgtgagct atggtcaaat gtcgtgctgt ttttcagtca ggccgctttt
     9241 gtgacattcg gaggtgctta tgcggtttta ccttatgttt cacagcaggc agtagagcat
     9301 tatcaatggt taggtcacct tcagatgatc gatggactgg ccctgggaga aactactccg
     9361 ggaccactga tcatggttct tgcctttgtg ggcttcatgg caggatatgg atactccggc
     9421 agcctggcca tgggaacact tggcttattc gcaaccacct attacacgtt tctaccctgc
     9481 tttctcttca tatttgccgg tgcaccctta attgaaaaaa cgcaacagaa tccaaatatc
     9541 aaagctgttt taggattagt gacggcagcc gttaccggtg ttattcttaa cctgacacta
     9601 tattttgccg aagcagtcgt atacggtaaa catatcacca cctggtcgga catacggccg
     9661 gaaaatatcc acccatttgc cttaatatgg atagtgattt cgttaatagc aatgggcaga
     9721 ttcaagatag gaatgatcac ctggataggc atcagtgcca ttttcgggtt gataacgtat
     9781 ctgttggcct gagcggaatt acgtaacttt gcagcttaga ttttttatgc ggtacttcaa
     9841 gatatttata ttcatcctga ttcccatatc actcattttt gtcttagaca aaggagtggc
     9901 cggattacct ccgttgggaa aactcatgga tccggtgcgg ggattcatgg ctaatgctga
     9961 aacctcatca tcgtataccg atgtggagat acctgctgag gagcatcatg tttcaggaac
    10021 tgtatatctg gatgagcgat tagtgccgca cattttcgct gcagacgaca ggtctttata
    10081 ttttatacaa ggatatgtaa ccgcaaaata tcgcttgtgg caaatggata ttcaatcgcg
    10141 cgcagcgggt ggacgattga gtgaaatttt tggtgaacgc tttattccct atgatctgga
    10201 acagcgtaga atgggcatgg tttacagcgc cgaaaaagct gttgaattat ggaaaaaaga
    10261 tactgttatg tatgcgaata ttcttgcata tacagaaggt atcaatgcct ttatagatcc
    10321 tttgcttgaa gagggatatg aagcctaccc tattgaattt aaattgctgg actacaagcc
    10381 tgaagaatgg acgccgctca attctgcact tctgttgcaa tatatgtcga gcacgctcac
    10441 aggatttgat gactacctcg aatattctaa cttgcgcaaa ctactcacac ctgaagaatt
    10501 tgctgcattg ttccctgatc gccctgaagg aattgatccg ataattccaa gcgaaaaagt
    10561 ttacaatttc actgttaatg atgctgattc tataccacaa gatgccagaa caggctattt
    10621 taaaaaggtg ctggaaattt ttgagagtca gcatgatcat acacaaatag gcagcaacaa
    10681 ttgggctgtt gacagcagca gatcgaaaag cggtcacccc atccttgcca atgatccgca
    10741 tctgcaatta aatctgcctt ccttgtggtt tgaaattcaa ttgcagacac ctgcaacaaa
    10801 ctgctatggg gtatcgctac cgggagctcc tgcaattgtc atcggattta atgaaaatat
    10861 cagctggggc gttaccaatg ctggaataga tgtgcgcgac tggtatacca ttgaatataa
    10921 agacgatacc aaatcagcat acaaatacgg aaaaacctgg aaaaaaactg agcaacgtat
    10981 cgaaacaatt gatgtgcgca atgcagatcc aatcattgac actgtgattt atacacatta
    11041 tggtcctatt gtgtatgaag atgaagaaat aactgccggc acaaaacaag atccacataa
    11101 agtgcaacgc atagacctgg caatgaaatg ggctgctctt gaaccgggag aacaaatcaa
    11161 aacattcttt tatctcaata gagcaaaaaa ttataacgat tatcgtcagg ctatcgccta
    11221 tttcaattgc cctgcacaga attttgtttt cgcctcacat aatggtgata ttgccatcac
    11281 acaacaaggt aaattcgccg ttaagaaaaa agaacaaggt cgctttgttt taaacggcag
    11341 cgatcctgga gatgattata ccgatttcat tcctttcgat cgcaatcccg gtattttaaa
    11401 tccgatgcgc ggattttgtt cttcagcaaa tcaacatcct acagatgcaa cgtatcccta
    11461 ttattataca ggatttgatt ttgagtttta tcgcaatcgc atcatcaatc gcagccttga
    11521 tgccatgcat gatgccacta tcgaaagcat ggagaaattg caacagaaca attacaatct
    11581 gcatgcaagc gaagctttac ctctcatgct agataaattg acctacgatg ttggcacacc
    11641 ggattcaaca gcatacactg taaagacagc attgtcggaa tggaatttca tgaacgatgc
    11701 tgacatgctc gccccaactt atttccagat gtggtgggat acattgtatg cgatgatgtg
    11761 ggatgaaatg gaagactctg tgatagcata cgtgcggccg aataaataca ataccgtaaa
    11821 agcgatgcat gcctttccga atgatcagcg cttgtttgat atgatttcta cacctgatca
    11881 aaaagaaaca ctcgatgaac ttatcaattt gagttatggt atcatggtgc atatggtaga
    11941 ttccctcgcc gctgaagata aaaatctgtt gcaatggtat cgacataaac gaactaatat
    12001 tatgcatatc agtcagattc cggccttcag taaaatgtcg gtgcacaatg gcggttatca
    12061 aagcatagtc aatgccacca gcgaaacgca tggtccaagt tggagaatga tagtagaaat
    12121 gtcgaatcct gttaaagcgg tgggtgtata tccgggaggt caatcaggca atcccggaag
    12181 taaatactac gacaattttg tcgatgactg ggcagcagga agatattatg aattgcatct
    12241 ttaccagagt gaagaagaag caaaagaaca agcacaattt acgatgcgat ttaattgata
    12301 tgaagtattt tgtaagtatc accataggct ttaccttgtg tatggcagga cagttcttcc
    12361 tgccatggtg gtggatattt gcaccgctta ccttcctcat ttcctttatt gtaaattatc
    12421 attcagcctg gcgtgcattt tttgcaggct tgttaattgt gtttatttcc tggatggctg
    12481 cttatattta taaagatctt gccaacgata gtattatgtc aggcaaaatg gccaatctct
    12541 tcaatttcaa aaataattac gcgctctttt ttatagtagc tattgccatg ggaattattg
    12601 gtggcctcac ttctaccgca gcctatttca ctcgaaattc gtaaaagatg tctgattttg
    12661 atgtgttgct gatcacggaa gatcgttatg aatcaccgca acatatcgat ggatacattg
    12721 ccaatatttt gcaagatgat gccattctga ctgcagcatt tgaagaggta ggattgcgca
    12781 cgaaacgcgt ttcatggagt aatccggaga tcgattggtc tgcttccagc tgcggcttgt
    12841 ttagaactac ctgggattat tttcttcgct tcaaccagtt taagaactgg atggagcaaa
    12901 ctgcacaaaa ggtaattttt atcaatccaa taaaagtcat tcgttggaat atggataaac
    12961 attatcttgc tgatctaaaa gctacaggca ttcgcgtggt tgacacgaca tttattgagc
    13021 aaaacacttc atgcgatctt gttgcattgc tgcaggcatc cgatgccgat tcaggtattg
    13081 tcaagcctgc tgtatcaggc actgcgcgac atacctatgt tgtagataat tcaagtcttg
    13141 aaagcgtgca gcccattgta aatgatttgt tgaagagcga ggcgctgttg tttcaaccat
    13201 ttcagaaaga cattttatta aacggagaat tatcgctgat catcatcgac ggcaaatgca
    13261 cacatgcagt aagaaagact gctaagcagg gcgattttcg tgtgcaggat gattttggtg
    13321 gcactgtgca tgatcatata ccaacaccgg aagaaattca atttgctgaa tcagcagttg
    13381 ccgcctgtcc ggagaaacca ttgtatgcac gcgtagatat cgtgcgcaac aatgccggtg
    13441 acctggccat tatggaactc gaattaatag aaccggaatt atggtttaga aaaaatcctg
    13501 cggctgcaaa gccattagct gcagcaataa aaaacattta tttctcatga ataaattatt
    13561 catcacatct tgtttaatcc tcattgcaaa tatttcaatg catgcacaag tttcaagtga
    13621 ctatgccgtt tt
//
LOCUS       DAOQOU010000084        13539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2349_length_13539_cov_6.647360, whole genome shotgun sequence.
ACCESSION   DAOQOU010000084 DAOQOU010000000
VERSION     DAOQOU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13539
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2349_length_13539_cov_6.647360"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLM90_08545"
     CDS             complement(<1..698)
                     /locus_tag="PLM90_08545"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HMZ89461.1"
                     /translation="MDRNNVIGFVLLGALIIAFFFVQSRYADNARVAEAAKQQTEDSI
                     KRVQDSLAKLELPKETAKQETTISALPDSLKNKMKPEEAKRLEDSLAAVQRANVFGSF
                     TPVMQGKEEITTLENDKLILKLSNKGGRIVQAEIKDHKNYHGGLVDVIGGDNNRFNYN
                     FYYEDNKMMNTEEMYFTPSLSSDGKTLTYTAKLSNGASLEQIYSFTNTEYLVDYNVRF
                     NGFRNVIPGRDPVI"
     gene            complement(910..2541)
                     /locus_tag="PLM90_08550"
     CDS             complement(910..2541)
                     /locus_tag="PLM90_08550"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMZ89462.1"
                     /translation="MKKEQARYIFVTGGVTSSLGKGIFSASLAKLLQARGFRVTIQKF
                     DPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLGVETSQANNVTTGAVYLSV
                     ITREREGEFLGKTVQVIPHITDEIQRRMLVLGENGENDIVITEIGGTVGDIESLPYIE
                     AVRQLKWKLGDSRCIVIHLTLVPYLQSAHELKTKPTQHSVKMLLESGLQADILVCRTE
                     HPLPKELRRKIALFCNVNINSVLEAADVKTIYDVPLNLLEEGADDIVLAKLKLPKKHE
                     PDLKNWKAFLSRLKNPTASVRIGLVGKYVELQDAYKSILESFIHAGAENECKVQVISI
                     QSEHITPQNVAEKLSDLDGVLVAPGFGDRGIEGKITAIQYVREHKIPFFGICLGMQCA
                     VIEFARNVLKMKDAHSTEMNPKTKHPVIDLMEEQKHVTEKGGTMRLGAYDCRLYKKTK
                     AFDIYKSQNISERHRHRYEFNNEYLKDYEAKGMMATGVNPQQNLVEIVELRDHPYFIG
                     VQFHPELKSTVENPHPLFVSFVKAAMDFKEKGPKN"
     gene            2841..3335
                     /locus_tag="PLM90_08555"
     CDS             2841..3335
                     /locus_tag="PLM90_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89463.1"
                     /translation="MEINERYIKASCGLTNGAYFEELLVDSMLEEGIPAKYRILSQFE
                     CSYQSDDEIRKEWPNKLYFKKTNDHYVWRADTLDQIRLAAETGYGRDLFKVDTLTGDT
                     IAVNMGYFVFNEASYTSCPVPFKPKTWYFLSIMQPDLLGVYLYVDEHKHFHVYEQESG
                     VSPI"
     gene            complement(3793..5277)
                     /locus_tag="PLM90_08560"
     CDS             complement(3793..5277)
                     /locus_tag="PLM90_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89464.1"
                     /translation="MTSTYKFDAVILHPYYTPTNVPTDAIELCNTNWLSIPLCLDGVT
                     YGTPWTYASYDTRLKCAFDGIMGFPVIDTDADGIWDTPAVGNFRSFVTSGLKEAMDFH
                     KAHLKFSTTDVGPEMKDIWYTEYNINSDERVTDAQVEYVGSILNTFAHAEGLWNWVLW
                     NVKSNAGISYRPNYLSSTTLQSFLSGTQIALMNTASQDEQAIMGLDEISAIAPCDGGT
                     VDCSSDGDCLDNSYYLRRLTYYAMQLLNKISMDDLVYLRSTALAYVGTPVNYNIPPTV
                     FYNGNPSDPKLIVFYTNVKDVDQTFGFNPGLVYQLFPGAVSVNLYGGTINFMNANVPY
                     TTSGKCSLYNFNNWYDCQAGGSGNMYSIETDENDILNGLASTDCPVGVSGAACVVVPK
                     YSLGYFTINIDVNYRLGENKDVFAIYPNPTSSNFYIQQINSEEMVATLLDVEVYNSYG
                     ANVLSTQVVEGQRIDISNLPVGSYNVLIKSNNVLRDSEVLVKMK"
     gene            complement(5356..6780)
                     /locus_tag="PLM90_08565"
     CDS             complement(5356..6780)
                     /locus_tag="PLM90_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89465.1"
                     /translation="MKNPICKSTFLSMRTFKHHVYYYLVGSLFSFLFFCSFHFNLYGR
                     GNLFIETKEIISNVLSPDSTFVDTIEFTAAFSEGDTIDMYVIGADTIYTIYATPLLDT
                     SALSFRYEASPMKIILSNQTFPVEQGLLGINVTDMFEPGHANEYVDLPKYTSGENPWD
                     YLSELCPTTLRVFSGQGSRFMELLGSARTAPNDPFNTSGTMMNGGYGFCIERIIPFFD
                     NTDNDIDAPPLFDDTPGAIPFSISDDMNDDGQLNTLTSWYLSDRYKNSFEDFYHKWEE
                     QPIYDPTDPAFDEYSEQPLYINQLIHLVNKIESENPGQSVSVMLCLNILSESATNCKN
                     IATYLKDDNGIDLAGIEIGNEVYFGWAEDLLGFRDNGFVPKFIHYWDYINGFNYDDFS
                     IGVDPDDGDPGDPTGDGMYFSDFDLSLVLPSDVLLDHDYLGVLKGDPSTANIPLGLPA
                     HNLPNCGEYVFITDESDTSNTLNM"
     gene            6951..9104
                     /locus_tag="PLM90_08570"
     CDS             6951..9104
                     /locus_tag="PLM90_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ89466.1"
                     /translation="MKEKLFLLLLFTVFWGCLYAQAPDIQWTKCLGGSSWDEGSACVQ
                     TPDSGVVVAGYRASFDGDCIDCPDYGYAFVVKLDSSGEIIWEKCYGGTTGEDIKSIIN
                     TTDGGLIFAGYTLSNDEDVTGNHGLSDYWVVKTDALGNLEWQKCYGGSGSDIARSIVQ
                     TIDGGYIILGRVTSEDGDVSGHHGVDSNNDYWVVKISELGEIEWEKTLGGSDEDWGES
                     IIQSQDGGFICAGYSNSDDGDIVGFHLYSDYWILKLNNEGELLWQHDFGGSSSDNAYA
                     LLEDENGDIIVSGVSYSNDGDVVGSHGASDAWVLKLNSEGNLIWNICYGGSLGDDFPS
                     VDYVSESRKILGGYSTSSNGDLSENYGSADSWIASIDSMGVLVWQKSVGGDTLDESFS
                     ISSVFDGGLVLVGYTTSNDLDASGNHGGRDIWVVKLAKPCDHQPYYADLDADSYGNPA
                     HYIYSCFDTMGYVENNLDCNDLDSLVHPFVEDICNNLDDDCDALKDEDAIALTWYLDN
                     DLDGFGNVEVDSLACFELSGYVFDANDCNDANALVNPGSIESCNEIDDNCNSEVDEGL
                     TYLHYYEDSDEDTFGNLAFDSISCAQPIGYVPDSADCNDLNPNIYPGAPEILNGLDDD
                     CDGLSDEGLSIADNTFNDVTIYPNPTNGIVTIELASEQIINVEVRTIDGKLVNTDIKV
                     VDKYHIIDLSSVAPGVYLIYIESPFWIKIVELVKN"
     gene            complement(9403..10761)
                     /locus_tag="PLM90_08575"
     CDS             complement(9403..10761)
                     /locus_tag="PLM90_08575"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89467.1"
                     /translation="MKLYQRFKHLIYLPCLLMMLPLSGFSQQSAWDIIKADAAPVLTT
                     PFADQFSAPEMVPLADYGWEDGLQVSYDGLHLYALYAPADLLSWVAYFAANPGLPICS
                     TLGSGAFIRDYAGDYGMDMYTNYFACDTFMNLDILYATRPSIDVPFSNWILSDIARPG
                     EIEGGPFPLFSDTDPDAVDHFLFTGPGSIWMINNTTANPSNIASAVRLPSPINPVSDE
                     FTADNPMLTRLENGNLLLIYEKYTNPDHRDFMFTISDDEGASWTTPTVITTINNDAGH
                     IEHPMLYFDGANYWFYYSQNFDIYRARQGAAGDWDSWTDHEAVILKGNTVGIGEPSIT
                     STGDIYFVAVIQNPDNPDDLMDADPWYVKNLFPIGIITNSFTAIEIHPNPADAFCTIN
                     TQVNDDYVCRITNLFGQEILSTKFIGKQMQMDVSTIPPGTYMLTLTGKKGDTWCQQMV
                     VM"
     gene            10962..12395
                     /locus_tag="PLM90_08580"
     CDS             10962..12395
                     /locus_tag="PLM90_08580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ89468.1"
                     /translation="MMRYFIATQDDFLHSWDERYHALVGKNLAKHPFEPTLKDNPILP
                     CNNLDWQSGNVWVHKQPIFLFQIAASIKVFGNSEMAVRFPSIVMGALLSLLIFRIGSI
                     TSNSLSGYFASGMFIFSFFQLEQTAGVIGMDHNDIAFAFYVTLSIWGYLEYLESGNKR
                     FVYMIALATAFGTLTKWLPALAIYGVWVIDAVLLDKQKRIHEIFLLFKSFCVSILIWG
                     SWQIYILLRWPIETKFEYEFNRRHFSEELEGHGGNWDYHIKMFDSQYGAYAFILLFVG
                     LLIFLLDKSKKRYKTLFLLIPISTYLVFSIAKTKSFAYVFYISPIVFTIIGYGLYQII
                     TLITAQVLNKVLKYVFYCAILIFYCNYQLSAKNLYKCHVLGQSEYINNYRSVLQHNAN
                     IYKQLDIVASDCDIIFNCRNMQIVDAEFYSDKTVYSWFPDSTTIDSLIDADYKIGAFQ
                     GFSNQQLPDYIKNNNKIQIIDLIQATE"
     gene            12516..13418
                     /locus_tag="PLM90_08585"
     CDS             12516..13418
                     /locus_tag="PLM90_08585"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HMZ89469.1"
                     /translation="MRLTAESHTSPLLCKAIIIACIGLVGVFACGKAQHGSEQQASIQ
                     QAPISIIDTSGENINTRIIPPAGYSRIPCDSLSFGYYLRHLPLKPHEASVHYYDGSIK
                     MNPNIYIAVVDQPIGNKDLHQCADAVMHLRADYLWQTGQYDRIHFDLTNGFRMEYAQW
                     MAGNRLVVSGNQTSWKKTAEASNTASDLWAYLEKVFTFAGTLSLSRELVSVERDDMQP
                     GDVFIKGGSPGHAEIVVDMAMDTITGKKIFLLAQSFMPAQETQILCNNEEPEISPWYN
                     LDFGTSLHTPEYWFNDTQLMRFQD"
BASE COUNT         3951 a         2516 c         2796 g         4276 t
ORIGIN      
        1 atgataacag ggtcgcgacc tggaattaca ttgcggaatc cgttgaaacg cacattgtaa
       61 tcaaccagat attcggtatt tgtgaagctg taaatctgct ctaaagatgc gccattgctt
      121 agtttggcgg tataggtaag tgttttgcca tctgatgaaa gggaaggagt gaaatacatc
      181 tcttccgtat tcatcatctt attgtcttca taataaaagt tatagttaaa gcgattattg
      241 tcgccaccaa ttacatctac caatccgccg tgataatttt tatgatcctt tatttcagcc
      301 tgaacaatgc gaccaccttt gttagataat ttaagtatga gcttatcatt ttcaagtgtg
      361 gtaatttctt cttttccctg cattacaggt gtaaaagagc caaatacatt tgcgcgttgc
      421 actgctgcca atgaatcctc gagacgtttt gcctcttcgg gtttcatttt gtttttcagg
      481 ctatcgggaa gtgcagaaat ggttgtttcc tgtttggcgg tttccttggg taattccaat
      541 ttcgccagcg agtcctgcac ccgtttgatg gaatcttcgg tttgttgctt ggctgcttct
      601 gctacacgag cattatctgc atagcggctt tgtacgaaga agaacgctat aatgagcgct
      661 cccagcaata caaacccgat gacattatta cgatccatta tactgttaaa ttgagcggca
      721 aagttacatc aaatcgctga tgtagcctat ataaagcccg gaagccctga aagacctatt
      781 ctgccttggt tttaagtgat gacgatcagc gcttaaaaac attgttaaaa gctcaatttt
      841 caagctggaa gttccgtaat gaccttgaga cctgatgtta tgccggaagc ctggttatca
      901 gcgaatgact tagtttttag ggcctttttc cttgaaatcc attgccgcct tcacaaatga
      961 aacaaacaac ggatgcggat tttctacggt tgattttaat tccggatgga actgaacacc
     1021 gatgaaataa ggatggtcgc gcaactcaac aatctctaca agattttgtt gcggattcac
     1081 acctgttgcc atcatacctt ttgcttcata atcttttaaa tattcattgt tgaattcata
     1141 gcgatggcga tgtcgctcgc tgatgttttg tgatttatag atatcaaaag ctttagtctt
     1201 tttatacaac ctgcaatcat atgctcccaa acgcatggtg ccgccttttt cggtaacatg
     1261 tttctgttct tccatcaggt cgataacagg atgttttgtt ttgggattca tttctgtgct
     1321 gtgcgcatct ttcatcttca acacattgcg tgcaaattca attactgcac attgcatgcc
     1381 gagacaaatg ccgaagaatg gaattttatg ctcacgcaca tattgtattg ccgtgatctt
     1441 accttcaata ccgcgatcgc cgaagcccgg agccaccagc acaccatcaa ggtcgctgag
     1501 tttttcggcg acattctgcg gggtgatatg ctcgctttga atactgatca cctgtacctt
     1561 acattcattt tcagcacctg catgaataaa ggattccaga atggatttat aggcatcctg
     1621 taattcaacg tatttaccaa caagaccgat gcgcaccgat gctgtaggat tcttcaatcg
     1681 cgataggaat gccttccaat tcttcaggtc cggttcgtgt tttttcggca attttaattt
     1741 tgcaagtaca atatcatcgg caccttcttc cagcaaattc aatggcacat cataaatagt
     1801 ctttacatcg gctgcttcga gaactgaatt gatatttaca ttacaaaaaa gtgcgatttt
     1861 cctgcgcaac tcttttggca atggatgctc ggttctgcat acaagaatat cggcctgcaa
     1921 accactttcg agcaacatct ttacagagtg ctgcgtgggt ttagtcttta gttcatgtgc
     1981 cgattgcaaa tagggaacaa gcgtgagatg tattacgata caacggctgt cgcccagttt
     2041 ccatttcaac tgacgaactg cttcgatata tggtaaagac tcaatatcac ccacagtacc
     2101 gccgatctca gtgatcacga tatcattttc gccattttca cccaacacaa gcatacggcg
     2161 ttgtatttcg tcggtgatat gtggaataac ctgcacagtc ttacctaaaa attctccctc
     2221 gcgctctcgg gtgatcacac tcagataaac ggcacctgta gtcacgttgt tggcctgcga
     2281 ggtttccact cccaggaatc gttcgtaatg gccaaggtca aggtcagttt cggcaccatc
     2341 ttcggtaacg taacattcgc cgtgctcata cggattcaag gtacccggat ccacgttaat
     2401 gtatggatcg aatttttgaa tggtcacacg aaaaccgcgt gcttgtaaaa gtttagctaa
     2461 tgatgctgaa aagatcccct taccgagcga agatgtaacg cctcccgtaa cgaagatata
     2521 gcgagcttgt tctttcttca taacgggctt taaaggtagg gtgaaaatca caatgttcaa
     2581 actcttcgag ggtgcagttt aacacattgc aacggcgttt tacaggccgc tttcctctga
     2641 aatcctacgc ctgtgcggaa aaatgatgag ggtaagatgt tgagaacatt ctgccatccc
     2701 tcccaacgaa aggaatggat ccttaccttc attggatgag cctcctccga ataacccgat
     2761 cgatcgccat acctgtgtgc gcaggttttt tgttctcagg ttgtggattg ctgctatcat
     2821 atgtgttcag accttgcaat gtggagatca acgaacggta tatcaaagcc tcctgcggac
     2881 tcactaatgg agcctatttc gaggaattgc ttgttgacag tatgctggaa gagggaattc
     2941 cggcaaagta taggatactt tctcaatttg aatgctccta tcaatctgat gatgaaatac
     3001 ggaaagagtg gccgaataaa ttgtatttca aaaaaacaaa tgatcactat gtttggcgtg
     3061 ctgatacatt ggatcaaatc aggctggctg cagaaacagg ttatggtcga gatctgttta
     3121 aagttgatac cctgacgggt gataccatag ccgtaaatat gggatatttt gttttcaatg
     3181 aagcatccta tacatcatgc ccggttccat tcaaaccaaa gacatggtat tttctaagca
     3241 tcatgcagcc cgatctatta ggcgtctatt tgtatgttga tgagcacaag catttccatg
     3301 tttatgaaca ggaaagtggc gtttctccaa tatgatcaga aaatgactaa tttttctatt
     3361 gcagagttgc ctgatggatc ttttgcttcg atgtaatata tgccaagtag tttaaaaatt
     3421 caaaaaaaat ggcagttgta ttcttgtttg taattttaca acgctaggta atttaattgc
     3481 aaattgaatt agcttgatat tgaaagaaag tgaatgccaa ctcaaaggct aatatgaggt
     3541 tgcaacgcta acctattcta tttaaaatat tacacctcac tttaatcgca tgtaggtcaa
     3601 gtccatgaac tatttgttgt cggttttctc tctgatttta tgttagtaaa ttttgattag
     3661 gttttgtttg taatgacata attagattgg aaaaagccaa ggccccgaat gaaattcgag
     3721 cccttggcaa tacctgtgaa aactaagcta tatttaaatt aattcttatg gcgtggttgg
     3781 aaatgatttt agttacttca ttttcactag aacttcgcta tctcgtaaaa cattatttga
     3841 tttaatcaaa acattgtagc tgccaacagg aaggttggaa atatctatac gctgaccttc
     3901 aaccacttgc gtagataata cattagcacc ataactgtta tatacctcaa cgtctaatag
     3961 agttgctacc atttcctctg aattaatttg ttggatgtaa aaattgcttg aggtggggtt
     4021 gggataaatg gcgaaaacat ccttgttttc ccctagtcga tagtttacat caatgttaat
     4081 tgtgaagtag ccgagtgagt attttggaac tacgacacaa gccgcaccgg aaactccgac
     4141 aggacaatcg gtactagcga gcccgttcaa tatatcattt tcatctgttt caattgaata
     4201 catattgcca ctacccccag cttggcagtc gtaccaatta ttgaaattgt atagggaaca
     4261 tttccctgaa gttgtatatg ggacatttgc attcataaaa ttaattgtac ctccatacaa
     4321 atttacagat acagcccctg ggaatagctg ataaactaat cctggattga agccaaatgt
     4381 ttgatcaaca tccttaacat tagtataaaa aacaataagc ttagggtctg atggatttcc
     4441 attataaaag acagttggcg ggatattgta attgacaggc gtgcctacat aagctaatgc
     4501 tgtgcttctc agatatacta aatcgtccat acttatctta ttcaacaatt gcattgcata
     4561 gtatgtcaac cgcctcaaat aatatgaatt atcaaggcaa tctccatcac tggaacaatc
     4621 aacagtaccg ccatcgcaag gagctatggc acttatttca tctagaccca ttattgcttg
     4681 ttcatcttga cttgctgtgt tcattaatgc aatttgcgtg ccgctcaaaa atgattgcaa
     4741 ggtagtgctt gaaaggtaat ttggtctata gctaatgcca gcatttgatt taacattcca
     4801 tagaacccaa ttccacaagc cttcagcatg ggcaaaggta tttagaattg atccaacata
     4861 ttcaacttgc gcatcagtaa ccctttcatc actattaata ttgtactctg tataccaaat
     4921 gtccttcatt tcaggcccaa catcagtagt gctgaattta aggtgtgcct tgtgaaaatc
     4981 catagcctct tttaagccgg aggtaacaaa gcttctaaaa tttccaactg caggtgtatc
     5041 ccatatgcca tcagcatctg tgtctattac aggaaatccc attatgccat caaaggcgca
     5101 cttaagcctg gtgtcatatg aagcgtatgt ccacggtgtg ccataagtta caccgtcaag
     5161 gcataaagga atgctgagcc aattggtatt acacagttca atagcgtctg taggcacatt
     5221 tgttggtgtg taatatggat gcagaatcac agcgtcaaat ttataggtag aggtcattgg
     5281 aatctgtgct gaatattttg cagctaattg gtcattccac aagggatatg tgcagtcagc
     5341 acaaggatct gcacctcaca tatttagggt gttcgaagta tcgctttcat cggtaataaa
     5401 tacatattcc ccacagtttg gcaaattatg agcaggcaat ccaagtggga tattagctgt
     5461 ggaagggtcg cctttcaata cgcctaaata atcatggtcc aataatacat cacttggcaa
     5521 tacaagtgat aaatcgaaat cactaaagta cataccatca ccggttgggt cgcccggatc
     5581 gccatcatcg ggatccactc cgatagaaaa atcatcatag ttaaaaccat ttatataatc
     5641 ccaatagtga atgaatttgg gaacaaatcc gttatcccta aagcctaaaa ggtcttcagc
     5701 ccatccaaaa tacacttcat ttccaatctc aattccggcc aagtctatgc cattatcgtc
     5761 tttcagatag gtggcaatat ttttgcagtt agtagctgat tcacttaaaa tattaagaca
     5821 tagcattacg ctcacactct ggccagggtt ctctgattct attttgttga ctaagtgaat
     5881 caactgatta atatacaatg gttgttccga atattcatca aaggcgggat cggttggatc
     5941 gtatatcggt tgttcttccc acttatggta gaaatcttca aaggaatttt tatatcggtc
     6001 tgaaagatac catgatgtaa gcgtattaag ttgaccatca tcgttcatgt cgtctgagat
     6061 actaaatgga attgccccag gtgtatcatc aaaaaggggc ggagcatcta tatcattatc
     6121 cgtgttatca aaaaaaggaa ttatacgttc tatacaaaat ccatatcctc cattcatcat
     6181 agtgccggat gtgttgaatg gatcatttgg cgcggttcta gctgaaccta ataactccat
     6241 gaatctactt ccttgcccgc taaaaacacg caaggttgtc gggcacagct cgcttaagta
     6301 gtcccaagga ttttcaccgg aagtgtattt tggcaggtcg acatactcat ttgcgtgacc
     6361 gggctcaaac atatcagtaa catttattcc aagtagtcct tgttctacag ggaacgtttg
     6421 attactgagt attattttca taggagaggc ttcatagcgg aatgataagg cagatgtatc
     6481 caacagcggc gtagcataaa ttgtgtatat ggtatccgca ccaattacat acatgtcgat
     6541 ggtatcgccc tcagaaaacg cagccgtgaa ctcaatagta tcaacaaaag tagaatccgg
     6601 tgataaaaca ttagaaatta tctctttggt ttctataaat aaattacctc ggccatataa
     6661 attaaaatga aatgagcaaa aaaacaggaa cgaaaaaagc gaaccgacaa ggtaataata
     6721 aacatgatgc ttgaatgtcc gcatactgag aaaggtagat ttacaaatgg gatttttcat
     6781 gatttgtgtt ttatggaatt aactgaatta aacaatagaa agaacggaat tttatactat
     6841 atccagggaa tagatattat gcccattgaa tatttgtgtt atccaaattt aataaattat
     6901 attactgttt gttaaaatct tttagtattt tggtgaaata atttcaataa atgaaagaaa
     6961 aactatttct tttattatta tttacggtgt tttgggggtg tttatatgca caagcccctg
     7021 atattcaatg gactaagtgc cttggtggct cttcttggga tgagggttcg gcatgtgtgc
     7081 aaactcctga tagtggagta gttgtagccg gctatagggc ctcattcgat ggagactgta
     7141 ttgattgccc agattatggg tatgcctttg ttgtaaaact cgattcaagt ggtgaaataa
     7201 tttgggaaaa atgctatggt ggaaccacag gagaagatat aaagtcaatt attaatacta
     7261 ctgatggggg actgattttt gctggttata ctctttctaa tgatgaagat gtgactggga
     7321 atcacggttt gagtgattat tgggtggtaa aaacggatgc cttaggaaat ctagaatggc
     7381 aaaaatgtta tggaggaagt ggtagcgaca tagcgagatc tattgttcag acaattgatg
     7441 gagggtatat aattttaggg agggtaacta gtgaggatgg agatgtgtca gggcatcatg
     7501 gtgttgattc caataatgat tactgggtag taaaaattag tgaattaggc gagattgaat
     7561 gggaaaaaac tttaggtgga tcagatgagg attggggtga atcaattatt cagtctcaag
     7621 atggtggatt tatttgcgct ggatattcaa attctgacga tggagatata gttggttttc
     7681 atctatatag cgactattgg atcctaaaat tgaacaatga aggcgaatta ttatggcagc
     7741 acgattttgg agggtctagt tctgataatg cttatgcatt gttagaggat gaaaatggag
     7801 atataatagt atcaggtgtt tcctattcaa atgatggaga tgttgttggg tcacacggag
     7861 catcagacgc ctgggtgttg aaactgaata gtgaaggaaa tttaatttgg aatatatgtt
     7921 atggtggttc attaggtgac gattttccaa gtgtagatta tgtttcggaa agtcggaaaa
     7981 ttctcggagg atattcaact tcatctaatg gtgatttgtc agaaaattat ggctcagcag
     8041 attcttggat tgcttcaata gattctatgg gggtcttggt atggcagaaa tcagttgggg
     8101 gagatacttt ggatgagtca ttttcaataa gttcagtttt tgatggtgga ttagtgttag
     8161 taggctatac aacatctaat gatttagatg cctctggtaa tcatggagga cgcgatattt
     8221 gggttgtcaa gcttgcaaaa ccttgtgatc accaaccata ttacgctgat ttagatgcgg
     8281 attcatacgg taatccggca cattacattt atagctgttt tgacaccatg ggttatgttg
     8341 aaaacaattt agattgcaat gatctcgata gcttggtaca cccatttgtt gaagatatat
     8401 gtaacaactt ggatgatgat tgcgatgccc tgaaagatga agatgccatt gcactgactt
     8461 ggtatcttga taatgattta gatggatttg gtaatgtaga ggtagactcc ttggcttgtt
     8521 ttgaattgtc cgggtatgta tttgatgcaa atgattgtaa cgatgctaat gcgttagtca
     8581 atcccggctc aattgaatct tgcaacgaaa ttgatgataa ttgcaattct gaagttgatg
     8641 aagggttaac ttacttgcat tactatgaag atagcgatga ggatacgttt gggaatttag
     8701 cctttgattc tatctcatgc gctcaaccga taggttacgt tcctgactcc gccgattgca
     8761 atgacctcaa ccccaacatc taccctggtg cacccgaaat cctgaatggt ttggatgatg
     8821 attgtgatgg gttaagtgat gaagggttgt caatagcaga taacactttc aatgatgtaa
     8881 caatctatcc aaacccaacc aatggtatcg ttactatcga attggcatca gaacaaataa
     8941 taaatgtcga agtgcgaacc attgatggaa aactggtgaa tacagatata aaggttgtgg
     9001 ataaatatca cattattgat ttgtctagcg tagctcccgg agtttattta atatatattg
     9061 aatcaccttt ttggattaaa atagttgagc tggtaaaaaa ctaaggcaaa caaatgtttt
     9121 gaatttcgag gataggctat attcattaag tttgttgatt caaatacatt tttttgcaaa
     9181 gcatgatatt acctgatgtt acatatctat cctagtttaa tttattgaat ttgagctcca
     9241 tccctaagac tatcgaaaat ttgtttttcc tttgacattg cacaactgtt tatcgtgtgt
     9301 tttgagggag ctactatagt gatttaagtg cttacaatgg ttgttatggt aactttcgcc
     9361 aaagatgcat ggcattagta tggttggaga cacctaaccc agttacatca ccaccatctg
     9421 ctgacaccat gtatcccctt ttttaccggt caaggtcaac atataggtgc ccggaggtat
     9481 ggttgaaaca tccatttgca tctgtttgcc gataaatttt gttgatagta tttcttggcc
     9541 gaacaggttt gtgatgcggc aaacataatc atcatttact tgcgtgttta ttgtacaaaa
     9601 tgcatcagcc ggattaggat ggatttcaat agctgtaaac gaatttgtaa taatcccaat
     9661 gggaaataaa ttttttacat accacggatc tgcatccatc agatcatcag gattatccgg
     9721 gttttggatg acagcgacaa aataaatatc acctgttgac gtaattgatg gttcgccaat
     9781 gcctaccgtg ttgcctttga ggatcacagc ttcgtgatct gtccaactgt cccaatcgcc
     9841 tgcagctcct tgtcgtgctc tatagatatc aaaattttgt gagtaataga accaatagtt
     9901 tgcaccatca aagtataaca tcggatgctc aatatgtccg gcgtcattat tgatcgtagt
     9961 gatcacggtt ggtgttgtcc aggaggcacc ctcatcatcg ctgatggtaa acataaaatc
    10021 gcgatggtcg ggattggtgt atttttcgta gatcaataat aaattgccgt tttccaatct
    10081 tgtgagcatg ggattatctg ccgtaaattc atctgatacc ggattgatgg gtgatggcaa
    10141 tcgaacggcg gaggcaatat tggaaggatt tgccgtagta ttattgatca tccaaatcga
    10201 gcccggtccg gtaaataaaa aatggtctac ggcatcagga tctgtgtctg aaaataaagg
    10261 gaatggtccg ccttcaattt ctcccggtct ggctatatct gataatatcc aattggaaaa
    10321 aggcacatca atgcttggcc ttgtggcata cagaatatcg agattcataa atgtatcgca
    10381 ggcgaaataa ttggtataca tatccatgcc ataatcaccg gcataatcac gtatgaatgc
    10441 gccgcttccc agtgtgctgc agatcggcaa accgggattg gcagcaaagt aagctaccca
    10501 tgataagagg tctgccggtg catataaggc gtacaaatgc aaaccgtcgt atgaaacctg
    10561 caagccgtct tcccatccat aatcagcaag gggcaccatt tccggcgcgc tgaattgatc
    10621 ggcaaaaggg gtagtaagga caggtgctgc atcggctttt ataatatccc aggcagactg
    10681 ttgactaaaa cccgaaagtg gtagcatcat cagtaaacat ggcaaataaa tcaaatgctt
    10741 aaagcgttga tataatttca tgtgtctaag ttagggaaat ttggtggatg gtatgaagcc
    10801 ttcaatataa aattattgct tgagagggtt tatatttgcg ttgaatgaaa cttgaatttt
    10861 taatctacat aagttttatt atttatggtc tcggatttac tgctcttttt tttaggagta
    10921 aagtaattgt tacctactcc tgcttattta tttcgtcttt tatgatgcgc tattttatag
    10981 caactcagga tgattttctt cacagttggg atgagcgata tcatgctcta gtgggaaaaa
    11041 atttggcaaa gcatcctttt gaacccacat taaaagataa tccaatttta ccatgcaata
    11101 accttgattg gcagtctgga aatgtttggg tacataaaca gcccattttt ctatttcaaa
    11161 ttgcggcaag tattaaggtg tttggcaatt ctgaaatggc agttcgattt ccaagcattg
    11221 ttatgggagc tttgttgtca ttgctaatat ttagaattgg tagtattaca agcaattctt
    11281 tgtcgggcta ttttgcatcg ggtatgttta tattttcttt ttttcagctt gagcaaacag
    11341 ctggggtaat cggaatggat cacaatgata ttgcatttgc cttttatgtt acgctttcta
    11401 tctggggcta ccttgaatat cttgaaagtg gaaacaaaag gtttgtatat atgattgctt
    11461 tggctacagc ttttgggact ttgacgaaat ggctgcctgc tttagcaatt tatggtgttt
    11521 gggtcattga tgcagtcctt ttagataaac aaaaaaggat tcatgaaata tttttattat
    11581 ttaagtcatt ttgcgtttca attctgattt ggggtagttg gcagatatat attttattgc
    11641 gatggccaat tgagacaaaa tttgaatatg aattcaacag acgacatttt tctgaagaat
    11701 tagaaggaca cggaggtaat tgggactacc atattaaaat gtttgacagc cagtatgggg
    11761 cttatgcttt tatactttta tttgtaggat tgttaatttt ccttctggat aagtcaaaaa
    11821 aaagatataa aactctgttt ttacttatac cgatttctac ttatttagta ttctcaattg
    11881 caaaaaccaa atcttttgca tatgtttttt atatttcacc tatagtgttc actataatcg
    11941 gctatggttt atatcagata attacattga ttacagcaca agttttaaat aaagtcttaa
    12001 aatatgtgtt ttattgtgct atattaatat tttattgtaa ctatcaactt tcagcaaaaa
    12061 atttgtacaa atgccatgtc ttaggtcagt ctgaatacat caataactat cgatccgttt
    12121 tgcagcacaa tgcgaatatt tacaagcaat tggatattgt tgcctctgat tgtgatatta
    12181 tttttaattg tcgcaatatg cagattgtag acgcggaatt ttattcggac aaaacagttt
    12241 attcgtggtt tcctgattca actactattg atagtttaat tgatgctgat tataaaatag
    12301 gcgcattcca aggtttttca aatcaacaat tgcctgacta catcaaaaat aataataaaa
    12361 tacaaattat tgatttaatt caagcaactg agtagttgca tttttatgtg attcagcctt
    12421 taaactattt ctaagaataa tgccctatta aattattaat ctcttgttaa aatacaaata
    12481 atcagacgaa acatttcctc cctacacatt ccatcatgag gctcaccgcg gaatcccaca
    12541 catccccact tctttgcaaa gccataatca tcgcttgtat tggtttagta ggtgtttttg
    12601 catgtggtaa ggcgcagcat ggatcggagc aacaagcttc gattcagcaa gcaccgataa
    12661 gtattattga tacttcgggg gaaaacatca acacgcgtat tattccgccg gcagggtatt
    12721 ctcgtattcc ttgcgatagc ctgtcattcg gatattattt acgccatctc ccactaaaac
    12781 cacatgaggc ttctgtgcat tattatgatg gaagcataaa gatgaatccc aatatttata
    12841 ttgctgttgt ggaccaaccc atcggcaaca aagacctcca tcaatgtgca gacgccgtca
    12901 tgcatttgcg cgccgattat ttgtggcaga cagggcaata tgatcgcatc catttcgacc
    12961 tgacaaatgg ttttcgcatg gaatatgcgc agtggatggc gggaaacaga ctagtggtat
    13021 cgggcaatca aacttcatgg aagaaaactg ccgaggcaag taatacagca tctgatttat
    13081 gggcttatct agagaaggta ttcacctttg ccggcacgct ttcgctttcg cgcgaactgg
    13141 tttctgttga acgagatgat atgcaaccgg gagatgtatt tattaaaggc ggaagtcccg
    13201 gtcacgcgga aattgtggtg gatatggcca tggataccat caccggaaaa aagatttttc
    13261 tccttgctca aagttttatg cctgcacagg aaacacaaat actatgtaat aatgaggaac
    13321 cggaaatcag tccctggtat aacctcgact tcggcacatc cttgcataca cctgaatatt
    13381 ggttcaatga tacgcaattg atgcgcttcc aggattaatg tgcaattttg cattattgct
    13441 atgtcactat ccattcgaag ttataatacg gttgatacct ttttgcagga tgcagcagtt
    13501 ttcttgcgac cattttcatt ggaaaataac cgcatttta
//
LOCUS       DAOQOU010000085        13462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2365_length_13462_cov_5.706198, whole genome shotgun sequence.
ACCESSION   DAOQOU010000085 DAOQOU010000000
VERSION     DAOQOU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13462
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2365_length_13462_cov_5.706198"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLM90_08590"
     CDS             <1..904
                     /locus_tag="PLM90_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ89470.1"
                     /translation="CNALDDNCDGIADEGLVPYFLYADMDDDGYGDADLDTLTCSDII
                     GFVYNNLDCDDLNTEINPAQSETCNGIDDNCNTLVDDDLTVYTLFVDNDGDGYGDPAF
                     VIDTCVMTVFGFVSNALDCNDTNMSIFSGAEDICNGLDDDCDGFVDENLTFLNQFQDL
                     DGDLYGNWSIDTLACMDIPGYVYDSTDCNDIDPNIYPGATELLNGLDDDCDGFSDEDL
                     GMNSNNSEQLIIYPIPASDIIFVDVASNEVSTLNIVNLRGEIVYQTSTWTGEVISISQ
                     LPPAMYMLQITTTNNHFAGYFLKK"
     gene            complement(1113..2294)
                     /locus_tag="PLM90_08595"
     CDS             complement(1113..2294)
                     /locus_tag="PLM90_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89471.1"
                     /translation="MKMLLCSLLLGISINLPAQDASILQEKWSYHLINQNFSRPTCAV
                     EFPNGRIMVGIYHSMMNNYNEFSTILVLDSAGNKIRSVVLSDTMNNYVYDMSLASDTT
                     AIIVGKAGPLGQNGTIPGDIYIAKLNVSGDIIWSRKYGSSDADEAKAVIPDGQGGAYV
                     CGRVGFGDGDVSTYYGNRNSADIWTLHIDASGNILWQQTMGGSSYDRPYAMLKLADGG
                     LLILGESASYDGNIESCEGGFDALMFKLDTIGNLVWKYSRGTKYNDTFIAAMVTDSLI
                     TAQLVQSEYVVRTGKTKREEFTITMDQHGEVLSQRKSDPDMIFENGEAYYYFKKDPDD
                     ENSTRCLTVAETEPRRTYCFPWEAQTQLEEVLQLHNGDLLLLVGVYVRTLNAFIVRIE
                     N"
     gene            2480..3205
                     /gene="bla"
                     /locus_tag="PLM90_08600"
     CDS             2480..3205
                     /gene="bla"
                     /locus_tag="PLM90_08600"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_132050774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HMZ89472.1"
                     /translation="MCLLAGFQCSTTGKVAFKPVEVYKTDALRITQISKHAYIHTSYK
                     QTDDFGLVPCNGVIITDKSECIILDTPVNDSCATLLINWVKQNLDVTINAIIPTHFHD
                     DCIGGLNAFHEQHIPTYAYTRTIEFAKQNNITIPEHSFSDSLELPIGDKKIIVKFFGE
                     GHTRDNVVAYFPAENILFGGCLIKSIDAGKGYLADANTAAWPETVHHVKTAFPEVQIV
                     VPGHGDYGDQRLLDYTIKLFSTK"
     gene            3329..5254
                     /locus_tag="PLM90_08605"
     CDS             3329..5254
                     /locus_tag="PLM90_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ89473.1"
                     /translation="MSPETLKLTMIIVGIILALVFYKFILRVFFGMVIVPEDRIGLVT
                     KKFVLFGANKELPDGHIIATKGEAGFQAHSLAPGLYFWKWIWQYEITMQPFIIIPTGQ
                     IGLVMAKDGVELETGRVLARKVECDSFQDAIAFMDGGGRKGRQTAIIAPGSYRINTFL
                     FDVEIVSMTNIPDNCVGIVTTLEGQPLDDGQIAGKIVEGHNRFQDADGFLINAGLKGL
                     QEQVILAGSYFLNPWFVKMEIVKMTEIPIGYVGVVISYVGPEGVDLSGTEFKHGNIVS
                     KGQKGVWADALGPGKYPINPYIMKVELVPTTNLVLNWASARTESHQLDKNLSTITVRS
                     KDGFTFNLDVSQIIHIPTYEAPKVIARFGNMANLVTQVLEPTIGNYFRNSAQDADVID
                     FLKMRKERQESAKQHIGKVLEQYNVNGVDTLIGDIIPPESLMKTLTDRKLAEEQKVTY
                     DTQMKAQETRRTLEKETAVADMQKELVKADQGVVIAEKIADASVKKATGDANGIRIQS
                     TAESDRLKLLANGEAEKIRVLANAEGEKVKLLADAEAERISKTGNAEAEKILAIGKSN
                     AESYKLSVEAMGGDNFTQLKITENIGVNKIKVIPDVLITGSGDSANGPISGLLGIELM
                     KNLAKRVNEGETDAKKE"
     gene            5229..5828
                     /locus_tag="PLM90_08610"
     CDS             5229..5828
                     /locus_tag="PLM90_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89474.1"
                     /translation="MKQMLKKNNINRYLLKNPVLLNGVFLFTILSWQACNNSADKKSA
                     EIYKQYPVYKTSDLDPEELSPLQFLQALSDEKSNILYVGLYPNGVTDNNIDFTAIKKV
                     PKNWIKPEHIDSLIIKFNDNTLTIPVFSVYADFAPKPGMHTTIGIEAMRMVEGYRNNM
                     YPSLWPEYYAEDINSIALQDKKQELINWWRQNSNQIVEH"
     gene            complement(5877..7331)
                     /locus_tag="PLM90_08615"
     CDS             complement(5877..7331)
                     /locus_tag="PLM90_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89475.1"
                     /translation="MFQNKSIVILRTFTNQEVRQFDEYLRSPFFNKNQTIVQAFDIIK
                     SWHPLYDHPNLERTQLFKLIFPNDTFEEQKLRYLMTDLTKHLEEYLCYKAISDENIFK
                     YHLLLYTYRIRRLDKAFLNTLKLAEKAIKDQPRRDVSHAFYEYLIEEDRYRFMSDRRE
                     HLHETNLQQVVDNLDKYYILNKMRYSAEIINNKNVVAINYRLFLYDEIMNHIRHNPLD
                     YVPAAKIYYNIILTLTEPENKIHYTNLLDLLKEHHQLFSKEEIFEMYVYAKNYCIRKI
                     NHGDIEYIRELFDLYKVMLKDRIIFKDNYLSQFDYKNIVYLGLRISEFDWVQQFIVKY
                     NEHLDPAIRANAYTYNMAYFHFFQNNYDEVLTLLRSVEFTDFYYHLDSKSLLLKTYFE
                     LEESEAFFSLIEAFKVYIKRNNLIPAYQKSGYTNMIKLTSKMFKWKLNPRNDLQDLVD
                     EFNNTKPVVDVLWIKKKLEDLQNRSEKLSGSWRK"
     gene            7600..8103
                     /locus_tag="PLM90_08620"
     CDS             7600..8103
                     /locus_tag="PLM90_08620"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89476.1"
                     /translation="MNRILITLAFLLTSAVMFAQTNARERSEDTNLPEIDNLSGLQPD
                     INDLNPSVATEVSTGSLENGNTEVGDEGASHTGSYTGDNIKLEGTNSLEEAQVYPNPA
                     TDYIYVNSGLSDGTIHVLNLLGQDMMQVAITSPVMSLNISELKEGIYFVSIENGANKV
                     VKKIKVL"
     gene            8674..8898
                     /locus_tag="PLM90_08625"
     CDS             8674..8898
                     /locus_tag="PLM90_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89477.1"
                     /translation="MKKLSGPFVLAAILAAHIGFVVATEESYTFRDDDAPSIKTEAPA
                     VDVSFSTLKGGSTANPNAMVSLTDNNTRIN"
     gene            8980..9606
                     /locus_tag="PLM90_08630"
     CDS             8980..9606
                     /locus_tag="PLM90_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ89478.1"
                     /translation="MRKWISILSLFTCISLRAQVATTDSLIQFSGLVMTSDSLMAVPY
                     AGIWSLTRNNGTTCDYNGFFSFVAAKGDSIRISALGYKDQVFTIPGDLTDTRYSVIQL
                     LTRDTIFVAETIIYPWPSKEEFRDAFLALDIPDDDYEVARKNLDREKLKDLGEAMAMD
                     ADMNADYQTKAIQQKIYYAGQYPPMRIFDVFAWKEFFEAWKRGDFKKE"
     gene            complement(9630..10205)
                     /gene="def"
                     /locus_tag="PLM90_08635"
     CDS             complement(9630..10205)
                     /gene="def"
                     /locus_tag="PLM90_08635"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ89479.1"
                     /translation="MILPIVAYGHPVLKQVAQPITADYPDLAKLIANMWETMYNGRGV
                     GLAAPQIGLDIRLFVVDSEQLKDDDDEEDDDVRLTKGIKKVFINAQIIEETGESWSYE
                     EGCLSIPDIRVKIMRNETLRIKYVDEHFVEHIETYDEMNARIIQHEYDHIQGVLLTDH
                     MTPLKRNLIKKKLDNIATGKISTKYKMKFAK"
     gene            complement(10207..10617)
                     /gene="ruvX"
                     /locus_tag="PLM90_08640"
     CDS             complement(10207..10617)
                     /gene="ruvX"
                     /locus_tag="PLM90_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HMZ89480.1"
                     /translation="MARILGIDYGTKRVGLAVTDPMQIIASPLTTVDTPQALDYIAKY
                     IAENEVERILIGYPTDLQGRPTDATFHVEGFIKAIQKRLPEMPLQKWDESYTSRMAMQ
                     SLVASGIKKKQRRDKKLLDQVSAAIILQEYLQNL"
     gene            complement(10620..11468)
                     /locus_tag="PLM90_08645"
     CDS             complement(10620..11468)
                     /locus_tag="PLM90_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HMZ89481.1"
                     /translation="MKKYFSLITFSHTVFALPFALIGFTLGITQPGVQFEWLDLVFVL
                     LCMVFARSAAMAFNRYIDADIDKDNIRTASREIPSGKISKSKALQFTIINCIGFVISS
                     GMLNVLCLYLSPIALLVILGYSLTKRFTWLCHFVLGIGLSLAPIGAYIAVTEHFQTLP
                     IIFSLAVFTWVSGFDIIYALQDEEFDKAHKLYSLPAKLGKFNSIIVSIIVHLNTVLLL
                     WYAGWYIEFGTIYWIGMGLFTTALVYQHLIVKPDDLSRVNRAFFSANGIASVVFGIFV
                     ITDLLV"
     gene            complement(11534..13207)
                     /locus_tag="PLM90_08650"
     CDS             complement(11534..13207)
                     /locus_tag="PLM90_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMZ89482.1"
                     /translation="MALSNKEVSKYFKILTGLLEVHGESDFRTKSYANAAFQLGRLSE
                     PVMTVDPAVLEKLPGVGKGIAAKIMELRETGTIAQLDELIQATPPGIIDILNIKGLGG
                     KKVRDIWTQLGIETVGELLYACYENRLSKLKGFGEKTQAAVIQSIEYYQNNLGKFHYA
                     AVAEYCDNILRQIHDLISDERIMFVGDLRRKCNIINAMEIIAPASADISSHISNINEI
                     VVEESSQSLIKGKTAHDMQFSILIVSDEDYVMQLFRLTGCEEHVNMILSLAGQGPFAS
                     EADIYSAAGLPFVFPEMREQFAEVDLMQFLHKATSPKHQLIELSDIKGVVHNHSTWSD
                     GKNTLEEMANACVEAGYDYLVISDHSKSAFYAGGLTEAQIVQQHSEIDKLNKQFTDFH
                     IFKSIECDILNDGGMDYDNDILATFDLIIASVHQNLKMDIEKATTRLLRAIENPYTRI
                     LGHMTGRLLLSRPGYPVHHKLIIDACAANGVAIEINANPYRLDIDYSWIPYAADKGVL
                     ISINPDAHSVEGIWDIRWGVQSARKGGLAGNQTLNTKTLQEFRNWLERK"
BASE COUNT         4068 a         2817 c         2592 g         3985 t
ORIGIN      
        1 ctgcaatgct ttagacgaca attgcgatgg cattgcagat gaaggattgg tcccctattt
       61 cttgtatgcc gatatggatg atgatggcta tggcgatgct gacctcgaca cacttacctg
      121 cagcgatatt attggttttg tttacaataa tcttgattgt gacgatctaa atacagaaat
      181 caatcctgcg caaagtgaga cttgcaacgg catagacgac aactgcaata ccctagtgga
      241 tgatgatctg accgtgtata ctttatttgt cgacaacgac ggtgatggat acggtgatcc
      301 tgcatttgtt attgacactt gtgtgatgac cgtcttcgga tttgtctcca acgcactcga
      361 ctgtaacgat actaacatga gtattttctc gggagctgag gatatttgta atggattgga
      421 tgacgattgc gatggttttg tggatgaaaa tctgactttt ctaaatcagt ttcaggattt
      481 ggatggagat ctttatggca actggtctat tgacacatta gcttgtatgg atataccggg
      541 atatgtatat gattcgactg attgcaatga tatcgatccg aatatttatc ccggtgcgac
      601 ggaattgttg aatggtttgg atgatgattg cgatgggttc agtgatgagg atcttggtat
      661 gaatagcaat aattcagaac aattgattat ttaccctatt cctgcatccg acatcatatt
      721 tgttgatgtc gcatccaatg aagtttcaac gctgaacatc gtgaatcttc gtggagaaat
      781 agtatatcag acctccacat ggacaggtga ggtaattagc atcagtcaat tacctcctgc
      841 aatgtatatg ttgcaaataa caacaacaaa caatcatttt gcggggtatt ttttaaagaa
      901 ataactgata cctgacatag cagggaattc aattaacaat ttgtatatct gtttattact
      961 cacgcggaga tacaaaagtg cacctgacaa aaaaatcaag cgcgacgatt caaatgcgca
     1021 tcaaacatag aatctttgaa taagtatgtg aacaatcgca tcttaacaga tgaataataa
     1081 caattaattg gtaaaaaggg aaataaatcc aatcagtttt caattcgaac tatgaacgca
     1141 ttgagtgtcc tgacataaac gcctaccaat aacaggaggt cgccgttatg aagttgcaaa
     1201 acctcctcga gttgtgtttg tgcctcccaa gggaagcaat acgtgcgacg aggctcagtc
     1261 tcagctacgg tgaggcaacg tgttgagttt tcatcatcag gatccttttt gaaataataa
     1321 tacgcctcgc cattttcaaa gatcatatcc ggatcggatt tacgttgtga taaaacctca
     1381 ccatgttgat ccattgtaat tgtaaattcc tccctttttg ttttacctgt tctcaccaca
     1441 tattcacttt gaacaagttg agctgtaatt aaagaatcag ttaccatggc ggcaataaac
     1501 gtatcattgt atttagtgcc tctgctatat ttccaaacaa gatttcctat tgtatccaat
     1561 ttaaacatta aggcatcaaa tccaccttcg cacgattcga tgttgccatc atacgatgcc
     1621 gattctccta atatcagtaa tccaccatca gcgagtttca gcatagcgta tggtctgtca
     1681 tatgaactgc cacccatcgt ttgctgccaa agtatatttc ctgatgcatc aatatgcaat
     1741 gtccatatat cagcagaatt acgattacca taataggtgg acacatcacc atcaccaaag
     1801 cctactcgac cgcatacata tgcacctcct tgtccatcgg gtatcacggc tttagcctca
     1861 tcggcatcgc tgctaccata tttcctactc cagataatat ctccggaaac attcagcttg
     1921 gcgatatata tgtctccggg tattgttcca ttctgtccaa gcggccccgc ttttcctaca
     1981 ataatagctg tagtatcgga ggcaagagac atatcataca catagttatt catggtatca
     2041 cttaggacta cacttctgat tttattgcct gcactatcta agacgaggat ggtagagaat
     2101 tcattatagt tattcatcat agaatggtaa atacccacca taatgcgccc attcggaaat
     2161 tcaactgcac aggttggtct cgaaaaattt tgattgatta aatgatatga ccatttctcc
     2221 tgcaatatcg atgcatcctg agccggcaaa ttaattgaaa tcccaagcaa taaactgcat
     2281 aatagcattt tcatgaatcc ctatttctgt aaatgtaagg tttaattatt gggaatggca
     2341 atcattaccg aaaattattt cacctcaaaa actgaaatta tatatcattt actaactaac
     2401 ttcacacctt taataccact tcatcataat aattaaagga ttgaaacgca tcaacttatt
     2461 tgtaacgaca ttatttgtta tctgtttgtt agcaggcttt cagtgcagca ccaccggaaa
     2521 agttgcattt aaacctgttg aagtatataa gactgatgcc ttgcgcatta cccaaatttc
     2581 aaaacatgcc tacatccata cttcgtataa acaaaccgat gattttgggc ttgttccgtg
     2641 caatggtgta atcattaccg acaaatcgga atgtattatt ttagatactc ctgtgaatga
     2701 ctcttgcgca accttgttga taaattgggt aaaacaaaac ttggatgtta caattaatgc
     2761 catcattcca acacattttc atgatgattg tattggcgga ttaaacgctt ttcatgagca
     2821 acatattcct acttatgctt atacaaggac cattgaattt gctaagcaaa ataatattac
     2881 aattcctgaa catagctttt ccgattcact tgaattaccg ataggtgaca aaaagatcat
     2941 tgtgaaattt ttcggtgaag ggcacacccg agataatgta gtagcatatt tccctgcgga
     3001 aaacatcctt tttggtggct gtctcatcaa gtctatagat gcaggaaagg gataccttgc
     3061 cgatgccaat accgccgctt ggcctgaaac cgttcatcat gtaaaaactg cattccccga
     3121 ggtgcaaatt gtcgttcccg gacatggtga ttatggcgat caaaggctac tagattatac
     3181 aatcaagctt tttagcacta aatgagcgtg attattgagt attttcatct aattacatgc
     3241 ttcaaaaaaa taatgcaata cctgaaaatt tcttccctac ctttacgtct tttatagctg
     3301 ttaatcacac taaaacaaat tggacactat gagcccggaa acacttaaac ttacgatgat
     3361 aatcgttggc attatcctcg cactcgtttt ctacaaattt attttgcgcg ttttctttgg
     3421 tatggtcatc gttcctgagg acaggatcgg acttgtaacc aaaaaattcg tcctcttcgg
     3481 cgctaacaaa gagcttcctg atggtcacat tatcgccacg aaaggtgaag ccggctttca
     3541 ggcacacagt cttgcgccgg gtctttattt ctggaaatgg atctggcagt atgaaatcac
     3601 tatgcagcca ttcatcatca ttcccaccgg tcaaatcggc ttggtgatgg caaaagatgg
     3661 cgttgaactg gaaactggtc gcgtattggc cagaaaagta gaatgcgatt cattccagga
     3721 tgctatcgcg tttatggatg gcggcggtcg taaaggtcgc caaacagcta ttatcgcacc
     3781 cggatcttat cgtatcaata ccttcctttt cgatgtggag atcgtgagta tgacaaatat
     3841 tcccgataac tgtgtaggaa ttgtaacaac acttgaaggt caaccacttg atgatggtca
     3901 gattgcaggt aagattgtag aaggtcataa ccgctttcaa gacgctgatg gcttcttaat
     3961 aaatgccggt ttaaaaggtt tgcaagagca ggtaatactt gccggtagct acttcttaaa
     4021 cccatggttt gtaaagatgg agatagttaa aatgacggaa attccaattg gttatgttgg
     4081 tgttgtaatt agttatgttg gtccggaagg tgttgatctc agtggaacag aatttaaaca
     4141 tggtaatatc gtttcaaaag gtcagaaagg tgtttgggca gatgctttag gtcccggtaa
     4201 atatcctatc aacccatata ttatgaaggt tgaattagtg cctaccacaa atctcgtgtt
     4261 aaactgggca agtgcacgca cggaatcgca tcagttagac aaaaatttga gcaccatcac
     4321 ggttcgttca aaagacggtt ttaccttcaa cctcgatgta tcgcaaatca tccatattcc
     4381 aacatatgag gcgcctaaag taattgcacg atttggaaat atggccaacc ttgttacaca
     4441 ggttcttgaa cctacaattg gtaactactt ccgtaactca gcgcaggatg ccgacgtaat
     4501 tgacttcctg aaaatgcgta aagagcgcca ggaaagtgcg aaacaacata tcggcaaagt
     4561 gcttgagcaa tacaacgtaa acggtgttga tactttgatc ggtgatatca ttccgcctga
     4621 atcattgatg aaaacactta ctgatcgtaa gcttgccgaa gaacaaaaag tgacttacga
     4681 cactcaaatg aaggcgcagg aaactcgacg cacgctggaa aaagaaactg ctgttgccga
     4741 tatgcagaag gaactggtaa aagctgacca gggtgttgtg attgccgaaa agatcgccga
     4801 tgccagtgtg aaaaaagcta caggtgatgc aaatggtatc cgcattcagt ctactgctga
     4861 atctgatcgt ttgaaattac tcgcaaatgg tgaggctgaa aaaatacgcg tattggcaaa
     4921 tgctgaaggt gaaaaagtaa aattgctcgc cgacgctgaa gctgaacgca tcagcaaaac
     4981 cggtaatgca gaagctgaaa aaattcttgc gattggtaaa tcgaatgccg aatcatataa
     5041 gctttcagtt gaagcaatgg gtggcgataa ctttactcaa ttgaagatca ccgaaaacat
     5101 cggtgtaaat aaaatcaaag ttattcctga tgtactcatc accggtagcg gcgacagcgc
     5161 caatggcccg attagcggat tgcttggaat cgagttgatg aaaaatcttg ctaaacgcgt
     5221 gaatgaaggt gaaacagatg ctaaaaaaga ataacatcaa tagatattta ttgaaaaacc
     5281 ctgttttact aaacggggtt tttcttttta ctatactttc ttggcaagca tgcaataatt
     5341 cagcagataa aaagtccgct gaaatctata aacaatatcc ggtctataaa acgtctgatt
     5401 tagatcctga agaattgtcg ccactacaat ttttgcaagc attaagtgac gagaaatcaa
     5461 atatattgta cgttggatta tatccaaatg gcgttacaga caacaatatt gatttcactg
     5521 ctattaaaaa ggtgccaaag aattggatca aaccggaaca catcgatagc ctaataatta
     5581 aatttaacga caatacatta accattcctg tattttcagt ttacgcagat tttgcaccta
     5641 agcccggaat gcatacaacc attggcattg aagccatgcg tatggttgaa ggatatagga
     5701 acaatatgta tccatccttg tggcctgagt attatgcaga ggatattaac tcaattgcat
     5761 tgcaggataa aaagcaagag ctgattaatt ggtggcgaca aaattccaat caaatcgttg
     5821 aacattaaga acggattgca aataaaagta aaaattagca agcaggtttg tgaagattac
     5881 tttctccagc ttcccgacaa tttttcagat ctattttgca aatcttcaag cttcttttta
     5941 atccataata catctacgac aggctttgta ttattgaact catcgacaag atcttgcaaa
     6001 tcgttgcggg gattgagttt ccacttaaac attttagacg ttagcttgat catgtttgta
     6061 tagccggatt tctgataagc agggatcaga ttattccgtt taatatatac tttaaaggcc
     6121 tcaatcaaac taaaaaatgc ttctgactcc tccaactcaa aataagtttt gagcagcaat
     6181 gatttcgaat cgaggtgata ataaaagtcg gtgaattcga ctgaccgaag gagcgtaagc
     6241 acttcatcat aattgttttg gaagaagtgg aaataagcca tattataagt ataggcattc
     6301 gctctgattg caggatcaag atgctcattg tatttcacaa tgaattgctg aacccagtca
     6361 aactcgctaa tgcgcaatcc caggtaaacg atattcttat aatcgaactg cgacaaataa
     6421 ttatccttaa aaatgatacg atctttcaac atcactttgt atagatcaaa cagttcacga
     6481 atgtattcta tatcgccatg gttgattttt ctgatacaat agtttttggc ataaacatac
     6541 atttcaaaaa tctcctcttt ggaaaataat tgatgatgtt cttttaacag gtcgaggaga
     6601 ttcgtgtagt gaattttatt ttcaggctcg gtgagtgtga gaatgatgtt gtaataaatt
     6661 tttgcagcag gaacataatc aagcggatta tgcctgatat gattcatgat ctcatcatac
     6721 aggaacaaac gataattaat ggccaccaca tttttattgt tgatgatctc tgcactatat
     6781 cgcattttat taaggatata gtacttatcc agattatcta ctacctgctg taggttcgtt
     6841 tcatgtaaat gctcgcgcct gtcgctcatg aaacggtatc tgtcttcttc aatcagatat
     6901 tcgtaaaacg catgcgacac atcgcgacga ggttgatcct taattgcctt ttcagcaagt
     6961 ttaagtgtat tcaaaaatgc cttgtcaagg cgacgaatgc gataggtata cagtagtaga
     7021 tgatatttga aaatattctc atcagaaatc gccttatagc acaaatattc ttccagatgc
     7081 ttcgtgagat ctgtcatcag gtatctcaac ttttgctctt cgaaggtatc gttcgggaag
     7141 atgagtttga acaattgcgt ccgctccaga ttaggatgat catatagcgg atgccaagac
     7201 ttgatgatat caaaagcctg cactatggtc tgattcttgt tgaagaacgg ggaacggagg
     7261 tactcatcga actggcggac ctcctggttc gtaaatgttc ttaaaatgac gatagattta
     7321 ttttgaaaca tcgcgaaaag cggttaaatt taacaatagt aactcagttc acagtccttc
     7381 cacaggattt ttctccgata caaaggggca aaattagggg gtggatgaat aacaaagtat
     7441 tgattatcaa taagttaagt atataaaata aatatatttg agaaaaaggg ttccgataaa
     7501 actgtgtttt tgtctttaac gtaagtgaag atgtgccgat aattttaaaa caaaatagga
     7561 tcaagcccgt attacttaag tatatttgac cacccaacca tgaacagaat tcttataact
     7621 cttgccttcc tgctcacctc agcggtgatg tttgcgcaaa ccaatgcacg tgaacgttcg
     7681 gaggacacca atctgcctga aatcgacaat cttagcggtt tgcagccgga tataaacgat
     7741 cttaatccat cagttgcaac tgaggtatct accggttcat tagaaaacgg caataccgaa
     7801 gtgggagatg aaggagctag tcataccgga agttataccg gcgacaatat caagcttgaa
     7861 ggaaccaatt cattggaaga agcgcaggtt tatcctaatc cggcaacaga ttatatttat
     7921 gttaattccg gattgtcaga cggaacaatt cacgtgctca acctgttagg tcaggatatg
     7981 atgcaagtgg caattacttc accggtgatg tcgcttaata tcagtgagct caaagaaggt
     8041 atatacttcg tgagcattga gaatggggcg aataaagttg ttaaaaagat caaggtatta
     8101 taaactttct gattaaacat taggggttaa atcaagcaaa agcctctccg acagccggag
     8161 aggttttttg tttttggtgg atttgagaga agccccggcc caaatggggt tatgcgagga
     8221 cttaaacctg ttaggtctaa gacctataaa agaccaacaa tcctatcatt tattattaga
     8281 ggtctgatag ccatttaagt gtgaaaaaca tatatctgaa tacaaagaaa aaatcggcct
     8341 aaaacataca atctcgactt gaaacctcag tgctacaagg gttgtgacag gtcttagacc
     8401 tgttaggtct taaacctgcc aggtttgaca ttttttggcg cgatttcatt agaggtctga
     8461 gcttcatttc accccctcca ctaccccatc cgataagcgc cattttttgt catcttttca
     8521 tcacttaaca cttcttaaaa ctcaataaaa tcgacatttc tcctcatttt aatgccctga
     8581 gatcccaaaa agtgacatga taccccctgc gtttttgtac ttggctgcgc cttcaatacg
     8641 gcggtatttt cgtcctatca aagcactcca attatgaaaa agctcagcgg tcctttcgtt
     8701 ctagcagcca tactggcagc acatatcggc ttcgtagttg ccacagaaga atcttatacc
     8761 ttccgtgatg atgacgcacc ttcaattaaa acagaagctc ctgcagtgga tgtgtccttc
     8821 tctacactta aaggtggctc aacagcaaat cctaatgcta tggtttcact gaccgacaat
     8881 aacacacgca tcaattaaac ttttagtgcc tgaacgcgtc tgttgtctga aaccgctctg
     8941 cagacaatac tgtatcttta ctcaaaacgt ttaggctata tgcgcaaatg gatctctata
     9001 ctctcactgt tcacttgtat ctccctgcgc gcacaggttg ctaccactga tagccttatt
     9061 caattctcag gtcttgtaat gaccagcgat tctcttatgg ccgtgccata cgccggtatt
     9121 tggtcgctga caagaaataa cgggacaact tgcgattaca acggattttt ctcctttgtg
     9181 gctgcgaaag gcgactcaat acgcatctca gcactcggat ataaggatca ggtgttcaca
     9241 ataccaggag atcttactga tacaagatat tctgtaatcc aactccttac gcgtgatacc
     9301 attttcgtag ctgaaacgat tatctatcct tggccatcta aagaggagtt tcgcgatgct
     9361 tttcttgccc ttgacatacc ggatgatgac tacgaagtag cacgcaaaaa cctcgaccgt
     9421 gaaaaattaa aggacctcgg cgaagcaatg gctatggatg cggatatgaa tgccgattat
     9481 caaactaagg ctattcaaca aaagatctat tacgcaggac aatatccacc aatgcgcata
     9541 ttcgatgtct ttgcctggaa ggaattcttt gaagcctgga aacgtggcga tttcaaaaag
     9601 gaatgatcaa agtaccataa atcgtctgct tatttggcaa acttcatttt gtacttggta
     9661 gagattttac cggtagcgat attgtccaat tttttcttga tgagattgcg ctttaatggt
     9721 gtcatgtgat ccgtcaataa tacgccttga atgtggtcgt attcatgctg aatgatgcgc
     9781 gcattcattt catcatatgt ttcaatatgt tcaacaaaat gctcatcaac gtattttatg
     9841 cgcagagttt catttcgcat gatcttcaca cgtatatcag ggatacttaa gcatccctcc
     9901 tcataactcc aagactcacc cgtttcttca ataatctggg cattaatgaa tactttcttt
     9961 ataccttttg tcaatcgcac atcatcatct tcttcgtcat catcatcttt cagttgctcg
    10021 ctgtctacaa caaataacct gatatccaag ccaatttgtg gagctgcaag tcctacacca
    10081 cggccattgt acatggtctc ccacatattg gcgatcaatt ttgccagatc aggataatcg
    10141 gcagtgatcg gttgtgcaac ctgctttaat accggatgcc cgtatgcaac aataggtaaa
    10201 atcatatcaa aggttttgta aatattcttg caaaattata gcagcgctca cttgatccaa
    10261 taatttttta tcacgtcgct gtttcttttt tattccactt gccaccaaac tctgcatggc
    10321 catgcgactg gtatacgatt catcccattt ctgtaaaggc atttcaggaa gtcgtttttg
    10381 tattgccttg ataaagccct caacatgaaa agtggcatca gttggtctgc cttgtaaatc
    10441 tgtgggatat ccaatcaata tcctttctac ttcattctca gcaatgtatt tggctatata
    10501 atctaatgcc tgcggcgtat ctaccgttgt cagcggagag gcaatgattt gcatagggtc
    10561 tgttactgca aggcccacac gctttgtgcc ataatcaata ccaagaatgc gtgccatatt
    10621 tataccaaca gatctgtgat gacaaaaatt ccaaatacca ccgatgcaat cccgttggca
    10681 gaaaaaaagg cgcgattcac cctactcagg tcatcaggtt ttacaattaa atgctgatat
    10741 accaatgcag ttgtaaataa gcccatgcct atccaataaa tagttccaaa ttcgatatac
    10801 cacccggcat accataataa taatacggta ttcagatgca caattatact aactatgatg
    10861 ctattgaatt ttccaagctt tgcaggtaat gaatataatt tatgtgcctt gtcaaattcc
    10921 tcatcctgca aggcataaat aatatcaaaa cccgataccc aggtaaatac tgcaagcgaa
    10981 aatatgatag gcaatgtttg aaaatgttct gtcaccgcga tgtacgcacc tattggtgcc
    11041 agtgaaagtc cgattccgag aacaaaatga caaagccatg taaaccgctt ggtcaaacta
    11101 tatcccaaaa ttacgagcaa ggcaattggc gaaagataaa gacacaatac attcaacatg
    11161 ccggaagata ttacaaaacc gatacaattg attattgtaa actgtaatgc cttcgatttg
    11221 cttatttttc cactaggaat ctctctggag gcagtgcgaa tattatcctt gtcaatatct
    11281 gcatcaatat acctattaaa tgccatcgcc gcactccgtg caaacaccat acataacaat
    11341 acgaaaacta aatccaacca ttcaaattga acgcccggtt gcgttatgcc taatgtaaat
    11401 ccaatgaggg caaagggcaa agcaaatacc gtgtgactga atgtgattaa tgagaaatac
    11461 ttcttcatcg aacacaaagt tacgacaatc cgagaggtaa acccttatca atcagctatt
    11521 tggtcgctga aacttacttg cgctctaacc aattgcgaaa ttcttgcaac gttttggtat
    11581 tgagggtttg attaccggca agtccgccct tccgtgccga ttgcacaccc catcggatgt
    11641 cccaaatacc ttcaacgcta tgcgcatcgg ggttaatgga gatgagcaca cccttgtctg
    11701 ctgcatacgg tatccaacta tagtctatat ccaatctata tggatttgca ttgatctcga
    11761 tagctacacc attggcagca caagcatcga tgatcaattt atggtgaaca gggtaacctg
    11821 gtctgctaag taaaagtcta cctgtcatat ggccaagtat gcgggtgtat ggattttcta
    11881 tggcacgcaa taaacgagtg gtagcttttt caatgtccat cttcaaattc tgatgcacac
    11941 tggcaatgat caaatcaaat gttgctaaaa tatcgttgtc gtaatccatg ccgccatcgt
    12001 tcaaaatatc acactcgata cttttaaata tatggaagtc agtaaattgc ttatttaatt
    12061 tatcaatctc actatgctgc tgaacgatct gcgcttctgt taatccgccg gcgtaaaatg
    12121 cagatttgct gtggtcagaa attacaagat aatcgtaacc ggcttccacg caggcattcg
    12181 ccatttcctc taacgtgttt ttcccatcac tccacgtact gtgattgtgc actactcctt
    12241 taatatcaga taattcaatt aattgatgtt tgggcgatgt agccttatgc aaaaattgca
    12301 ttaaatctac ctctgcaaat tgttcacgca tttccgggaa tacgaatggc aatcccgctg
    12361 ctgaataaat atccgcttct gatgcaaatg gcccttgccc cgcgagcgat aatatcatgt
    12421 tcacatgctc ctcgcaccct gtcaaacgga acaattgcat cacataatct tcatcagaaa
    12481 caataagtat gctgaattgc atatcatgtg ctgtcttacc ttttattagc gattgcgatg
    12541 attcttcaac cactatctca tttatgtttg atatatgaga tgaaatgtca gctgatgcag
    12601 gtgcaatgat ttccatcgca ttaataatat tacatttcct gcgcagatcg ccgacaaaca
    12661 ttatgcgctc atctgaaatc agatcatgta tttgccttaa tatattatcg caatattccg
    12721 caacagcagc gtagtggaat ttgcctaaat tattctgata atattcaata ctctgtataa
    12781 ccgctgcctg tgttttttcg ccaaaacctt tcaacttcga caacctgttt tcatagcacg
    12841 cgtatagcaa ttcaccaacg gtttcaatgc caagttgtgt ccagatatct cgtacttttt
    12901 taccgccaag gcctttgata ttaaggatat caattatccc cggaggcgtt gcttgtatca
    12961 attcatccag ctgcgcgata gtgccggttt cccgcaattc catgatcttc gctgctattc
    13021 ccttccctac tccgggtagt ttctccagca cagcagggtc tacagtcatc acaggctcac
    13081 ttaatctacc caattgaaat gcagcattgg catagctctt agtcctgaaa tccgactcgc
    13141 catgcacttc aagcaatcct gtaagaattt taaaatattt tgatacttct ttgtttgata
    13201 atgccatgag gtaaaaataa gaggtatgta cgaatatcct gaataaacga taacgagccg
    13261 gttgttctga ggggagatct aaagcgctga gggtggtaaa tacatgtttc tacgtaggca
    13321 catcataatt tcacgttgcc atcatgcaca cctcccacac agcgcggtgc gactcccgcc
    13381 tcagcgggac ttccacgccc cagtactctg attacccttt atctttacca catgctttcc
    13441 acccacgaac accagctcgt tg
//
LOCUS       DAOQOU010000086        13408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2375_length_13408_cov_6.995582, whole genome shotgun sequence.
ACCESSION   DAOQOU010000086 DAOQOU010000000
VERSION     DAOQOU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_13408_cov_6.995582"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..2320)
                     /locus_tag="PLM90_08655"
     CDS             complement(131..2320)
                     /locus_tag="PLM90_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ89483.1"
                     /translation="MSSQVLLITPPFTQLNTPYPATVYLKGFLNTLGISSRQADLGIE
                     TTLKLFTRRHLQKVFDLAEEHINNDDRHYQLWEQRDTYIDVAESVVAFLQGKLPALAH
                     QIVSGNMLPVGERIAQVDKSDLAYQQLDLQDKAKHLATLYLEEIADFIRDTIDPYFGF
                     TRYAERLARSANSFDALYEALNQDYTYVDTLMCELLDEHIHHSQPTLIAITAPFPGNV
                     YAAFRCAQHIKNNYPNISIAFGGGFVNTELRSLKDVRVFEFFDFISLDDGEAPIACLW
                     KYINGEITIDYLKRTFILQNNTVQYINNASCADYAFADTGTPDYSDLPLDKYIHAIEI
                     INPMHNLWSNGRWNKLTMAHGCYWGKCTFCDVSLPYIADYSPLSAGMIVDRMESMIAQ
                     TGETGFHFVDEAAPPALMREVAVEILRRQLNVTWWTNIRFEKSFTRDLCVLLMASGCI
                     GVSGGLEVASDRLLALIKKGVTVEQVAIVLRNFTDAGIMVHAYLMYGFPSQTDQETID
                     SLEIVRQLFDADVLQSAFWHRFAMTAHSPVGLQPEVFNVNAHRAHNIAFADNDVEFTD
                     ITGGDHEKFAEGLRISLYNYMQGAGLDLPLQKWFNHGIPKTTITPDHINRILDRNEIN
                     IPKATQRVIFLGLLLSASTRTKSKRGNIYTETTLQFQFEENICELKLPEPHASWFQSI
                     LPMLHPAQPQQTISALQNLYAQQGLEDFELFWVNELSGLYEVGLLVI"
     gene            2426..2734
                     /locus_tag="PLM90_08660"
     CDS             2426..2734
                     /locus_tag="PLM90_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HMZ89484.1"
                     /translation="MEKKWYASALGRFRVVAILEGISFLLLLGIAMPLKYAANIPEAV
                     EYAGWMHGLLFISYMFTLLQVKIEHEWTFGKAALAVLASILPFGPFILDKRILQRESA
                     "
     gene            2970..5759
                     /locus_tag="PLM90_08665"
     CDS             2970..5759
                     /locus_tag="PLM90_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ89485.1"
                     /translation="MNLRLTLCSIVTLMAFTLSAQLISTDPIFPTPDGPVTVIFDASQ
                     GNAALAGYTGDVYAHTGLITQDSDTPSDWQYVQGVWGTADPEVLMTSLGDDKYSITFS
                     SIMDFYGAAADDTIYQMAFVFRNAAGTIVGRDVDGSDIFVNVYAPGLNVAITVPDYNP
                     YLVNPGDNVHIEASSTYSDSLYLYINGVEIIAGEDSNITYDLTAVDPGLSDIVVKAVS
                     GADVMYDTTYFYVLGTVTTEAVPAGMHQGINYIDDNTVTLVLYAPYKNYVFAIGEHSD
                     WLLTDETFMKRDPDGATYWITLSGLEPGKEYPYQYFIDGNLTVADPLAEKVLDPWSDG
                     YINEATYPGLIDYPTGKASGIVSVFQTAQPDYVWAVPDFTSPPTEKLVIYELLIRDFI
                     AAHNYATLTDTISYLKTLGVNAIELMPIMEFEGNESWGYNPSFFIAPDKYYGTKEALK
                     IFIDSCHANGIAVILDIAMNHAFGQCPLVQMWWDATANAPALNSPYFNQVPTHDYNVG
                     YDFNHESDATEAFRNIVFTHWITEYHVDGYRFDLSKGYTQTNTLGNVAAWGAYDASRI
                     AIWENIGSVIWAVNPDAKLILEHFADNSEEETLSNAGFMLWGNMNYNYNQGTMGYGGS
                     DLNWASYQSRGWDDPHLVTYMESHDEERLMYKNLTYGNTTNPDYNVKNLNIALSREEL
                     AGAFLFTIPGPKMLWQFGELGYDYSIEYGCRVCNKPIRWDYYSNANRLRIYQVWSELI
                     KLKTTYPAFSTTNYNMSVAGFGKRINLNDPDMNVTVLGNFDVNLANISPNFQHAGWWY
                     EYFSGDSLNVSDVLASFTMAPGEYRLYTDVRLSTPDIIEGINHVDEMANNSIIAMPNP
                     TEGETEILTTLQTADDVVLEFYNNTGSMIERYDLGKQAEGIFSFTWNSNAPAGIYFCR
                     LVYGDNAGVVKISKL"
     gene            complement(5808..8318)
                     /locus_tag="PLM90_08670"
     CDS             complement(5808..8318)
                     /locus_tag="PLM90_08670"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMZ89486.1"
                     /translation="MNFGFQYARALVVMGFCLITSSLFAQNRTINGNVRDKKTNESVL
                     FAGIYLLHTETAAQTDEKGNFSLIAPSGYDTIIVTSIGYITDTIVLGRELTQQLNVAL
                     DQSLYELNTMVITAQEEPAKTIMRKVIENKKINDQQHLAAYKTRGYNKIEIDLGNLEM
                     LTDYDSIPVDTTKKEKKPNPYAVLLDHIDSTSDVTHFLPFFFTETVSDFYYRKNPKTR
                     KEIIIASRTSGINNASATQILGNYYQQFNLYDNYWFLLSKNFVPPITDAWNFFYRVDL
                     ADSAWIDNDWCYLIRFQPRQQQENTFHGFMWIADSSFAVKEISMQLDSAVNINYCKRA
                     QFYMLFDHYNDSAWVLKEDRLLVEFLPIKNSTSFIGRRTTYYDDYNFDPNVFEEVVKY
                     KDDIVYTDSVVNNDEAYWRSVRPDSLTYNETGVYELVDSLKNIKSFMTVVDVYNTLMY
                     GYWNLGYVRIGPFANMASSNDVEGFRVRIGGKTGDLISKRLTIGGYGAYGFQDRTFKY
                     GAEFHAVIAKKPWQEFDLKYYDDLDVSNNDNVTFGEDNLLSGFYRRRDTPQKIVDIEK
                     FQVSYKKDWIWGLSNTITLTSTHMHPLFDIYYYDNDSLRSNITNSEIKFGLRFAYREK
                     FMFDNYRRYSLGTKYPKLELYYRIGIPDLMESDFRYTAFEVNIWDFFTVGSIGGTSYT
                     LKAGKIFGTLPTLLLETPPGNETYFMNHANFNLMNEYEFVNDTYAELFLTHSFYGFFL
                     NKIPYVNKLKLREVATFKLAYGSLSDANVELNDYLKNSAPYYIGNTSMTNLPYMEAGI
                     GVENIFKLIRVDAIWRLTYRNNPLAPNFGVRLGVNLDI"
     gene            8442..9599
                     /locus_tag="PLM90_08675"
     CDS             8442..9599
                     /locus_tag="PLM90_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89487.1"
                     /translation="MINYRTLCLVIGINFSAFFAQSQNATFEVTLGGFDAEAEAAFNY
                     ATDQWSQYLISDVPIKVHANLVTLLPGQLGITFPNGEKNFVGAPFNDTWYASCLANAI
                     TGTELNPGYADMDIFINSTANWYFGTDGNTPAGQYDFVSTVMHEICHGLGFLSLSNKD
                     VTTGSFGLIYATDFFPLVTSFPWPELDTLPSAMDHYLSNGSGTMLLTFENPSDALGDE
                     FTSNNIFCTSEKVLNAHGGEAGRIYAPSTFTLGSSMSHWNEGSYPVGNENEFMTPNAA
                     SGHSSQVPGPMTLALLEEIGWEIDYTIDINTTIQPDESPIIFPNPANENAVISNLPVN
                     ASWDVVSLDGRNVASGANNVIDCKDWARGIYIVRFISDRGAEIKSSKLVLN"
     gene            complement(9639..11480)
                     /locus_tag="PLM90_08680"
     CDS             complement(9639..11480)
                     /locus_tag="PLM90_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HMZ89488.1"
                     /translation="MKIKNLLLICFLACTSVISAQQIDQICPPNWFIGMRDSSVQLLI
                     HGTGIGNYDLKINYPGVRLVAVTRVENKNYLFADLVIDKTARPGAVDLYFSAGKKSFT
                     KQWALQARAPWTNTYGLDAGDLIYLLMPDRFANGDTTNDVVASMNETISDRKDPKGRH
                     GGDIAGIMQHLDYISKLGATAVWCTPLVENNEPKWSYHGYAITDHYKIDPRFGNNQQY
                     AEFVQSAHKHNLKVVVDLVHNHVGDQHWFIKDLPMPDWIHQWDTSFVRSNYRTSVKND
                     PYASAYDVKKMNEGWFDKHMPDLNQENPYLAKYIIQNNIWWIETYHVDGFRLDTYPYS
                     DLSFLQDWGVAIQKEYPGFGIFGEVWVNGVGVQGYFHGDNHINDGFNSHLPGVTDFNL
                     YDATTTGLNENFGWYEGMTRIYHNLTQDYLYGDVMKNVVFLGNHDLSRFYSVMGESPE
                     KFKLGVAFAMTTRGMVQWYYGDEILMKNFSFPEDGLVREDFPGGWKSDGTNKFDPQNL
                     AGKEKEIFNYVSGLANWRKNSDAIKYGKLMQFIPENGVYVYFRYTEKETIMIVLNSAS
                     TEYTLDTKRFAERIGNFTIAQDVVTKQNNTIAGLKVAAKSPGVYVLK"
     gene            complement(11610..>13408)
                     /locus_tag="PLM90_08685"
     CDS             complement(11610..>13408)
                     /locus_tag="PLM90_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ89489.1"
                     /translation="LIAVTALAISGFTLSSCFNDLDLAPPSGINAETVYNNPDNYVHV
                     LAKLYAGLSISGNQGPAGNPDIAGIDEGFSQYIRVLWNLQEATTDAAKCAWNDPGIPE
                     LNKMQWSSTNSFVNAMYYRIYFQIPLCNEFIRESADAKMSERGFSDAEQTTIRGYRTE
                     ARFLRALSYYHALDLFGNVPFITEDDVVGAFYPERITRAELFNYVESELLAVATELPA
                     PRANEYGRADRAAAWTLLARLYLNAEVYTGTARYTDCATYCQKVITEGGYSLEPVYDH
                     LFMSDNQGSNEVIFPVTFDGQFTQTYGGTTFLTHSTVGGDSLSQIDKAYYGIEFGWGG
                     NRSTPEYASFFPDANDARNMFKTFGQTLEFTEDAQLGQFVVGYPIAKWRNVLSTSVAE
                     FTATISGDVMTVTSVADGSVQVNQNLAGSGIAGENKIIEFITGTGGTGTYRLANADNV
                     TSPTTIHAAIPGTNLDAGGNYTDIDFPMMRLADVYLMYAECAARGAGDAGLGVEYVNM
                     LRERAYGDVAHNVGSITPESVLEERARELQWEGYRRTDLIRYGLFTGGSYLWAFKGGP
                     FNGTSVPETLNLFPIPAADVVANPNLVQNPGY"
BASE COUNT         3881 a         2710 c         2830 g         3987 t
ORIGIN      
        1 gtgttcgtgt gcgggctgat ttgtggaatg tgcttaagcc ggcaatccgt gtaatccttc
       61 aatcccaata atccgtgatt cagacaataa ttgtgctccg aattgaacta ataaatttat
      121 tcccggttgc ctaaatcacc aataacccaa cctcatacaa accgctcagc tcattcaccc
      181 aaaacaattc aaaatcttcc aacccttgtt gagcatacaa attttggaga gctgatattg
      241 tttgctgagg ttgtgccgga tgtagcattg gtagtataga ttgaaaccat gaagcgtgtg
      301 gctccggtaa tttcagctcg catatatttt cttcgaattg aaattgaagt gtggtttcgg
      361 tataaatgtt tcctcttttt gatttagtgc gcgtggatgc gctgagtaat aatcccagga
      421 aaataacgcg ctgcgttgct tttggaatat ttatttcatt tctgtctagg atgcgattta
      481 tgtggtcggg tgtaattgtg gtttttggga taccgtgatt aaaccatttt tgtaaaggaa
      541 gatcaagtcc ggcaccctgc atgtaattgt atagcgatat gcgcaatcct tcagcaaatt
      601 tttcgtgatc gccaccggtg atatcggtaa attctacatc attatcggca aatgcaatat
      661 tgtgtgcccg atgtgcattt acgttaaaaa cttccggctg caatccaacg gggctatgtg
      721 ctgtcatggc aaagcgatgc caaaaggctg attgcaaaac gtcggcatca aaaagctgac
      781 gcacaatttc cagactgtcg atcgtttcct gatctgtctg tgatggaaag ccatacatta
      841 aatatgcatg caccataata ccggcatcag taaaattgcg cagcacaatt gcaacttgtt
      901 caactgtaac gccttttttt attaaagcaa gtaatctgtc agatgcaact tctaaaccac
      961 cacttactcc aatacatcca ctggccatca gcaatacaca gaggtcgcgg gtgaagcttt
     1021 tttcaaatcg gatgttagtc caccaggtaa catttaattg cctgcgcaaa atttcaacag
     1081 ccacttcgcg catcaacgcc ggcggggcgg cctcatcaac aaaatgaaat ccggtttcac
     1141 ccgtttgtgc tatcatggat tccatgcgat ctactatcat gcctgcggag agcggactgt
     1201 aatctgcaat atagggcaac gacacatcac agaaagtgca tttaccccaa taacaaccat
     1261 gtgccatggt gagtttattc catcgtccgt tgctccacag gttgtgcatg ggatttatta
     1321 tttctatcgc atggatatat ttatccaaag gcagatcgga ataatcggga gttccggtat
     1381 cggcaaaagc ataatcggca caagatgcat tgttgatata ctgcacggta ttattctgta
     1441 aaataaatgt gcgttttaaa taatcaatgg ttatttcacc attaatatat ttccataaac
     1501 aggctattgg tgcttcgcca tcgtctaaag aaataaaatc aaaaaattca aacacacgca
     1561 catctttaag gctgcgtaat tcggtgttca caaagccacc gccaaatgca attgaaatgt
     1621 ttggataatt atttttgatg tgttgagcac atctaaaagc cgcatataca tttcccggaa
     1681 acggcgctgt tatggcaatg agtgttggtt gggagtgatg aatgtgttca tcgagcaact
     1741 cacacatcaa cgtatcaaca taagtatagt cttgatttag cgcctcgtat aatgcatcaa
     1801 atgaatttgc cgatctcgcc aggcgctcgg cataacgtgt aaatccgaaa tacggatcta
     1861 tggtatcgcg aatgaaatct gcaatttctt ctaaatataa agttgcgaga tgctttgcct
     1921 tatcttgtaa atctaattgt tgatatgcta aatcagactt atccacttgt gcaattcttt
     1981 cgccaactgg aagcatattg ccacttacaa tttggtgtgc caatgccggc aattttcctt
     2041 gaagaaatgc aacaaccgat tcggctacat caatatatgt atcgcgctgc tcccatagtt
     2101 ggtagtggcg atcatcatta ttgatatgct cttctgctaa atcaaaaact ttttgcaggt
     2161 gtctgcgggt aaataatttc aatgtcgttt caatgccaag atctgcttgt cgcgatgaaa
     2221 taccaagcgt atttaaaaaa cccttcaggt acaccgtggc cggataaggc gtattgagtt
     2281 gggtgaaagg aggggtgatg agcagaacct gggaggacat gggtaaaaat aagaagaaag
     2341 aagaaaggag agaggagaga ggagagagga gagaggagag aggaaagagg agggggtttg
     2401 gtagttttgt tcaaaattta ggtgtatgga gaagaaatgg tatgcttcgg cgttggggcg
     2461 gtttagggtg gtggcgatat tggaggggat ttcattcctg cttttactgg gtattgcgat
     2521 gccattaaaa tatgctgcca acatacccga agcggtggaa tatgccggtt ggatgcatgg
     2581 cttgttgttc attagctata tgttcacatt gcttcaggtg aaaatagaac acgaatggac
     2641 ttttggtaaa gccgcgctcg ccgttctggc ttcgatactt ccctttggac catttatttt
     2701 ggacaaaagg atattgcagc gcgaatctgc atagctgtcg ttagttgcat ttttcgcaaa
     2761 tatgcgcttc gaatgtggcg aacatttgca tttttcgcaa attatattga tttcgttatt
     2821 gattgtaaat tgacgagtcg caaaacggcg aatatttttg aaagtgatta tatataagtc
     2881 ggtttgttta acatttgtct ctacattttg caggcaataa ttttcatgct gcggttgagg
     2941 ttataacctt tgcacagtaa ctttactgta tgaatttaag gcttactctt tgcagcattg
     3001 tgacgttaat ggcgttcacg ctttcagcgc agctcatttc cacggatcca atttttccaa
     3061 ctcctgatgg accggtaact gtaattttcg acgcatcgca aggtaatgcc gcacttgccg
     3121 gttataccgg tgatgtttat gcgcatacgg gtcttatcac ccaggacagc gatacgccaa
     3181 gcgactggca atacgttcaa ggtgtttggg gaactgcaga tccggaagtg ttgatgacga
     3241 gtttgggtga tgataaatat tccatcacat tttcaagcat catggatttt tatggcgccg
     3301 ccgcagacga tacaatttat caaatggcat ttgtttttcg aaatgctgca ggaacaattg
     3361 tcggcagaga tgttgatggc tccgatattt ttgtaaatgt atatgcaccc ggtttgaatg
     3421 ttgctattac ggtgcctgat tataatccat atctggtaaa tccgggcgac aatgtacata
     3481 ttgaagcaag cagcacttac agcgatagtt tatatctcta tattaatggc gttgaaataa
     3541 ttgcgggtga agacagcaat attacatacg atttaactgc ggttgacccg ggattatccg
     3601 atattgtggt gaaggcggtt tccggtgccg atgttatgta cgacactact tatttttatg
     3661 ttttaggaac agtaactaca gaagcggttc cggcaggtat gcatcaggga ataaattata
     3721 ttgatgacaa taccgttact ttagtgttat atgcgccata taaaaattat gtttttgcca
     3781 tcggagaaca tagcgattgg ttgctgaccg acgaaacatt tatgaagcgc gatcccgatg
     3841 gtgccactta ttggatcaca ttaagcggac ttgaaccggg aaaagaatat ccttatcaat
     3901 attttattga tggcaattta accgtcgctg atccgctagc agaaaaagta ttggatcctt
     3961 ggagcgatgg ttatatcaat gaagcaacat atcccggact tattgattac ccgacaggaa
     4021 aagccagtgg catcgtttcg gtatttcaga cagcacaacc cgactatgtg tgggcggttc
     4081 cggattttac ctcaccgcca acagaaaaat tggttattta tgaattgtta atccgcgatt
     4141 ttattgccgc acataattat gcaactctca cagatacgat ctcatattta aaaacacttg
     4201 gtgtaaatgc tattgaattg atgccgatta tggaatttga aggcaatgag agctggggat
     4261 ataatccaag tttcttcatt gcacctgata aatattacgg cacaaaagag gcacttaaaa
     4321 tatttattga cagttgtcat gccaatggta ttgccgtaat tttggatatt gccatgaacc
     4381 atgctttcgg acaatgtccg ttagtgcaaa tgtggtggga cgcaacagca aacgcaccgg
     4441 cattaaattc tccatatttt aatcaggtgc caacgcatga ttataacgtt ggatatgatt
     4501 ttaatcacga aagtgatgcc acagaggcgt ttagaaatat tgtattcaca cattggataa
     4561 cagaatatca tgtagatggc tatcgcttcg atctttcaaa aggatatacg caaacaaata
     4621 cattgggtaa cgttgccgca tggggagcat atgatgcatc acgcattgct atttgggaaa
     4681 acattggcag cgtaatttgg gctgttaatc cggatgctaa attgattctg gagcattttg
     4741 ccgataacag cgaagaagaa acactcagca acgcaggatt tatgttgtgg ggaaatatga
     4801 attacaacta caatcaaggc accatgggtt atggcggatc tgatttgaac tgggcaagct
     4861 atcaatcgcg cggatgggat gatccgcatc tggtaacata tatggaaagt cacgacgaag
     4921 agcggttgat gtataaaaat cttacttatg gtaataccac taatccggac tacaatgtaa
     4981 aaaatttaaa tattgcgctt tcaagagaag aattagccgg tgcattttta tttacaattc
     5041 ccggaccgaa aatgttgtgg caattcggtg agttaggata tgactatagt attgaatatg
     5101 gttgtcgcgt atgtaataaa cctattcgtt gggattacta ttccaacgca aatcgcctgc
     5161 gcatttacca ggtgtggagt gaattaatta agttgaaaac aacatatccg gcattcagca
     5221 ctacaaacta caatatgagt gttgcgggtt tcggaaagcg cattaatttg aatgatcccg
     5281 atatgaatgt taccgtgtta gggaattttg atgtgaacct ggcaaatatt tcacccaact
     5341 ttcaacatgc aggatggtgg tatgaatatt tcagcggaga ttccttaaac gttagcgatg
     5401 tacttgcttc atttaccatg gcgccgggag aataccgatt gtataccgat gtgcgcttgt
     5461 cgacgcctga cattattgaa ggcataaatc atgttgatga aatggccaac aattcaatta
     5521 ttgctatgcc aaatccaact gaaggagaaa ctgaaatatt gactacactg caaacggccg
     5581 atgatgttgt gcttgaattt tataacaaca ccggatcaat gatcgaaagg tatgaccttg
     5641 gtaaacaggc agaaggaata tttagcttta catggaatag caatgcacca gccggtattt
     5701 atttctgcag gttggtttac ggcgataatg caggggttgt gaaaatcagt aaactttaaa
     5761 catagaaacc gctagacaaa aagtatcatg ttttttatag tggcctctta gatatccaga
     5821 ttaacaccaa gtcgtacgcc gaaattcggt gcaagtggat tattgcggta tgtgagtcgc
     5881 cagatggcat ctacgcgaat gagtttaaaa atattctcta cgccaatgcc cgcttccata
     5941 tatgggagat tggtcattga agtgttgcct atataatatg gggcactatt ttttaaataa
     6001 tcatttagct caacatttgc atcgctcaga gaaccgtatg cgagtttaaa agttgccact
     6061 tcacgcaatt ttagtttatt tacataaggg attttattga ggaagaaacc gtagaaacta
     6121 tgcgtaagga ataattcggc atacgtatcg tttacaaatt catactcatt catcaggttg
     6181 aaattggcat gattcataaa atatgtttca ttgcccggag gcgtttccaa caataatgtt
     6241 ggcaaagtgc cgaatatttt gcccgctttg agcgtatagc tggttccgcc gatacttcca
     6301 accgtaaaga aatcccagat atttacttca aaagcagtat atctgaaatc actttccatt
     6361 aaatcgggta taccaatgcg gtaatagagc tcaagtttcg gatattttgt tcccaggcta
     6421 tacctgcgat aattatcgaa cataaatttt tcacgatagg caaatcgcaa accaaattta
     6481 atttctgagt ttgtgatatt gcttcgaagt gaatcgttat cataataata aatgtcgaac
     6541 aacggatgca tgtgtgtgct ggtgagtgta attgtatttg agagtcccca aatccagtct
     6601 tttttatagc tcacctgaaa tttttcaata tcaacaattt tttgtggtgt atcgcgacga
     6661 cgataaaaac cggatagcag gttatcctcg ccgaaggtta cattatcatt gttgcttaca
     6721 tccagatcat cataatattt taaatcaaat tcctgccaag gctttttggc aataacggcg
     6781 tgaaattcag caccatactt aaatgttcta tcctgaaatc cataagcacc gtaaccgcca
     6841 atagtcaacc tcttgctgat aaggtcgccg gtctttcctc caatacgcac tctaaatccc
     6901 tctacatcat tactgctcgc catgttggca aatggcccaa ttctaacata gccgagattc
     6961 caatatccgt acattaaagt attatacaca tcaacaacgg tcataaacga tttaatgttc
     7021 ttcaggctat ctaccaattc atatacgccg gtttcattgt atgtcaggga atcaggacga
     7081 acactacgcc aataagcttc atcattattt acaacgctat cggtatatac aatatcgtct
     7141 ttatatttaa caacctcctc aaatacattt ggatcgaaat tgtaatcatc ataataggtt
     7201 gttcgtcggc cgataaagct tgttgaattt tttattggaa gaaactctac caacagtctg
     7261 tcttccttca acacccatgc cgaatcatta taatgatcga atagcatata aaactgtgca
     7321 cgcttgcaat aattgatatt tacggcactg tcaagctgca tggaaatttc tttcacggca
     7381 aaagaactgt cggcaatcca cataaagcca tgaaaagtat tttcctgctg ttgacgaggt
     7441 tgaaagcgaa tcagataaca ccagtcgtta tctatccacg cagaatcggc aagatcaacc
     7501 cgatagaaga aattccatgc gtcggtgata ggcggaacaa aattcttact tagcaagaac
     7561 caataattat catatagatt aaactgctga taataatttc ccaggatctg tgtggcgctg
     7621 gcattattga tacccgaagt gcgactggca ataattattt cttttctggt tttcggattt
     7681 ttcctgtaat agaaatctga aacagtttcc gtaaagaaga atggcaggaa atgcgtcacg
     7741 tcgcttgtgc tatcgatatg atcgagcagg accgcgtatg gatttggttt tttttctttt
     7801 ttagttgtgt caaccggaat ggaatcataa tcggtaagca tctcgagatt gccgaggtca
     7861 atttctatct tgttataccc gcgtgtttta tatgctgcca gatgttgctg atcgtttatt
     7921 tttttgtttt caatcacctt gcgcataatt gtttttgcag gttcttcctg cgcggtaatc
     7981 accatggtat ttaattcata aagcgattgg tcgagcgcca cattcaattg ctgcgtcaat
     8041 tcgcgtccaa gtacaatggt atctgtgata tatcctatgc ttgttacaat aatagtgtca
     8101 tatccggaag gcgcgatcag cgagaaattg cctttttcat cagtttgtgc ggctgtttct
     8161 gtgtgaagta gataaattcc ggcaaagagc acagactcat ttgttttctt atcgcgcaca
     8221 ttcccgttga tggttctgtt ttgagcaaaa agagaacttg tgataaggca aaaacccatc
     8281 accaccaaag cgcgcgcata ttggaatcca aaattcatgc ctacggccat aaaattaccg
     8341 aaaacccccg tgaaatcaga tgccggatgt caccatttcg atgcgatgta aggaaaatac
     8401 gcgtttatct gattagtttt gaaaggtcac aacaccttgc tatgattaac taccgcaccc
     8461 tttgccttgt tatcggaata aatttttcag cattttttgc tcagtcacaa aatgccactt
     8521 ttgaagtcac tttaggtgga tttgatgcag aagccgaagc cgcatttaac tacgcaacag
     8581 accaatggag tcaatacctg attagtgatg tgccgataaa agtgcacgcc aatttagtga
     8641 cactgctccc cggacaactg ggcattactt ttcctaatgg tgagaaaaat tttgttggcg
     8701 caccatttaa tgatacctgg tatgcaagtt gcctggcgaa tgccataaca ggaacggaat
     8761 taaatccggg atatgccgat atggatattt tcatcaacag tactgcaaat tggtattttg
     8821 gaaccgacgg caatactccc gcggggcaat atgattttgt gtcgacagtg atgcatgaaa
     8881 tttgtcatgg tttgggattt ttaagtttga gcaataaaga tgtaactaca gggtcattcg
     8941 gacttatata tgcaacggac ttctttccct tggtgacatc atttccatgg ccggaattag
     9001 atacgcttcc ttcggcaatg gatcattatt tatcaaatgg ttcaggtacg atgttgctca
     9061 catttgaaaa tccatcagat gccctgggtg atgaatttac aagtaacaat attttttgta
     9121 caagtgaaaa agtgttgaat gcgcatggtg gcgaagcggg aagaatttat gcaccttcaa
     9181 catttacgct tggcagcagc atgtcgcatt ggaacgaagg ttcatatcct gttggcaatg
     9241 aaaatgaatt catgacaccc aatgcggcat caggacattc cagccaggtt ccgggtccta
     9301 tgacactggc attattggaa gaaattggat gggaaattga ttataccatt gatataaata
     9361 cgactattca accggatgaa tcaccaataa tatttcctaa tcctgcaaat gaaaatgcag
     9421 tcatttcaaa cctgcctgtt aacgcaagtt gggacgttgt gtcgcttgac ggtcgcaatg
     9481 tagcttccgg agcgaataat gtaattgatt gcaaagactg ggcgcgcgga atatatatcg
     9541 tcagatttat ttctgatcgc ggtgcagaaa taaaatcctc aaaactcgtg cttaattgag
     9601 acttaaatta cagtgatttt agaatcgacc ttaatatttt atttcagcac ataaaccccc
     9661 gggcttttag ccgccacttt tagtcctgcg atagtattat tttgtttggt tacaacatcc
     9721 tgtgcaatcg tgaaattgcc gattctttct gcaaaccgtt ttgtgtcgag tgtgtattct
     9781 gtagatgcac tattgagtac aatcattatt gtttcttttt cagtatatcg gaaatacaca
     9841 tacacgccat tttcgggaat aaactgcatg agtttgccat attttatggc gtcagaattt
     9901 ttgcgccagt tagctaatcc gcttacataa ttaaatatct ccttttcttt tcccgcgaga
     9961 ttttgcggat caaatttatt tgtgccatcg ctcttccatc ctccgggaaa atcctctcgc
    10021 accaatccat cttcaggaaa agagaaattc ttcatcaata tttcatcgcc gtaataccat
    10081 tgtaccatgc cacgggtggt cattgcaaag gcgacaccca atttaaattt ttcaggactt
    10141 tcgcccatca ccgaataaaa gcggctcaag tcgtggttgc cgaggaacac tacattcttc
    10201 attacatcgc catagaggta gtcctgagtg agattgtgat atatgcgcgt catgccttca
    10261 taccatccga aattttcatt taacccggtt gttgtagcgt catacaaatt gaaatctgtt
    10321 acacccggaa gatgcgaatt aaatccatcg ttgatatggt tatcaccatg aaaatatccc
    10381 tgcacaccaa caccgtttac ccatacttct ccgaaaatgc cgaatcccgg atattctttt
    10441 tgtatggcaa cgccccaatc ctgtaagaaa gaaagatcgg aatacggata ggtgtctaac
    10501 ctaaatccat caacatgata tgtttctatc caccagatat tattttgaat aatatatttt
    10561 gccagataag gattttcctg atttaaatca ggcatatgtt tatcaaacca tccctcattc
    10621 atttttttta catcatacgc gctggcataa ggatcgtttt ttacacttgt gcgataatta
    10681 cttctaacaa aacttgtatc ccattgatga atccaatccg gcatagggag atctttaata
    10741 aaccaatgtt gatctcctac atgattgtgc accagatcaa caactacttt cagattgtgt
    10801 ttgtgtgccg attgaacgaa ttcagcgtat tgttgattat tgccaaatcg cggatctatt
    10861 ttataatgat cggtaatggc gtatccatga taactccatt ttggttcatt gttttcaacc
    10921 aacggcgtgc accaaactgc agttgctccg agcttgctga tataatccag gtgttgcatg
    10981 ataccggcaa tatcgcctcc atgtcttcct tttggatcct tgcgatcgct tattgtttcg
    11041 ttcatgcttg caacaacatc atttgtggtg tcaccatttg caaagcgatc gggcattaat
    11101 aaataaataa gatcaccggc atcgagtcca tatgtatttg tccaaggggc ccttgcttgc
    11161 agtgcccatt gtttggtgaa tgattttttt cctgctgaaa aataaaggtc tactgccccg
    11221 gggcgagcgg ttttatctat aacaagatct gcaaacaagt aatttttatt ttctacacgt
    11281 gttacagcaa cgaggcgaac acccggataa ttgattttga gatcataatt tccgattcct
    11341 gttccatgaa ttagtagttg cacactggaa tcgcgcatac cgatgaacca gtttggcgga
    11401 cagatctgat caatttgctg tgctgatatg acacttgtgc aagccagaaa gcaaattagc
    11461 aggaggtttt tgatcttcat gcagtaaaaa tagttgaata tgaggataac acttcccctc
    11521 aggaaaggtt tttgtaatta agtgcaattg cgaaaggaca aaaaaaagcc cccgacaatg
    11581 cggaggcttt tgaaagaata tttaatatat caatatcccg gattttgaac gaggttagga
    11641 tttgctacaa catctgcagc aggaatcggg aacaagttca gtgtttcagg aactgaagtg
    11701 ccgttgaatg gaccgccttt aaacgcccag aggtaagatc cgccggtgaa cagtccgtaa
    11761 cgaataaggt ctgttctgcg atatccttcc cattgcaact cacgagcgcg ttcttccaac
    11821 acagactcgg gagtaattga gcctacatta tgtgcaacat cgccataagc gcgttcgcga
    11881 agcatattta cgtattctac acctaatccg gcatcacctg caccgcgagc ggcacattcg
    11941 gcgtacatca gataaacatc ggcaaggcgc atcattggaa aatcaatatc ggtgtagttg
    12001 ccaccggcat cgaggtttgt tcccggaatt gccgcgtgaa tggtggtggg agatgtaacg
    12061 ttatcagcgt tggccagtcg ataggttcct gttccgcctg ttcctgtgat aaattcgatg
    12121 atcttatttt caccggcaat accacttcct gccaggtttt gattcacctg cacggagcca
    12181 tcggcaacag atgtaacggt cattacatcg cccgaaatag tagcagtgaa ttcggcaact
    12241 gaagttgaca atacattgcg ccattttgct atcggatagc caacaacaaa ttgtccaagc
    12301 tgtgcatctt ctgtaaactc gagtgtttgt ccaaatgttt tgaacatatt acgcgcatca
    12361 tttgcatcag ggaagaaaga tgcgtattct ggcgttgagc gatttcctcc ccaaccaaat
    12421 tcaataccat aatatgcttt atcaatttgg ctcaaagaat cgccaccaac agttgaatgt
    12481 gtcaggaatg ttgttccacc ataagtttgt gtaaactgac catcgaaagt tacagggaaa
    12541 ataacctcgt ttgaaccttg attatctgac ataaacaaat gatcataaac aggttcaaga
    12601 gaataaccac cttcggttat cactttctga caatatgttg cacaatcggt atagcgagca
    12661 gtgccggtat atacctcggc attgagatac aaacgagcca gcaaagtcca agcagcagcg
    12721 cgatcggcgc gaccatattc atttgcacgt ggtgcaggaa gttcggtagc gactgctaat
    12781 aattcagact caacataatt aaatagctcg gcacgtgtta tcctttcggg atagaaagca
    12841 ccaacaacat cgtcttcagt aatgaatgga acatttccaa aaaggtccaa tgcgtgataa
    12901 taactcaatg cacggaggaa acgcgcttcg gtacgataac cgcgaatagt agtttgctct
    12961 gcatcagaaa atccgcgctc agacattttt gcatctgccg attcgcggat aaattcatta
    13021 cacaacggaa tttggaaata aatacgataa tacatcgcat ttacaaatga gttggtactt
    13081 gaccactgca ttttatttaa ttcaggaata cccgggtcgt tccacgcgca ttttgcggca
    13141 tcagttgttg cctcttgcag gttccacaat acgcgaatgt attgcgagaa accttcatcg
    13201 ataccggcga tatcaggatt acccgcaggc ccctggttgc cggaaatgga aagccctgcg
    13261 tataatttag ccaatacatg gacataatta tccggattgt tgtacaccgt ttcggcattg
    13321 atgccacttg gcggcgccag gtcaaggtcg ttgaaacatg agctcaacgt aaaaccggaa
    13381 atggcgagtg cggtgactgc aattaata
//
LOCUS       DAOQOU010000087        13367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2387_length_13367_cov_7.242037, whole genome shotgun sequence.
ACCESSION   DAOQOU010000087 DAOQOU010000000
VERSION     DAOQOU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2387_length_13367_cov_7.242037"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLM90_08690"
     CDS             complement(<1..268)
                     /locus_tag="PLM90_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HMZ89490.1"
                     /translation="MIQIEIWSDVVCPFCYIGKRRLERALAGFAHAGQVEITWRSFLL
                     DAQLVTEPGKSINQHLSERKGWSMEQTREINAYVTEMAAGEGLDY"
     gene            397..2133
                     /locus_tag="PLM90_08695"
     CDS             397..2133
                     /locus_tag="PLM90_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89491.1"
                     /translation="MLTGHLKAQATYTAADIALDSAVHHYNAYRNTLDAFTPANITDE
                     KINLVESNCNIIVREFESIMALNANPQSEVAKYFHAMTKYEYGFVLGMAGKNDKAYRI
                     MSEIEDYMQKQGSFSFPKKYLYDGKNYVIQWDNFAPTLHEYFTGMAEITINLGMNEKT
                     MPYARSAMSLQYPNTGDNAVWYRYIAASKYMQSSKNIGYYAYDGFEVALNFLDWLYSL
                     DADYQKLVNDYDYPRQVTAYNYILLVEKGYPATNTGENWLKAATAFMKFNESAYAGYC
                     FNRAADLKYDNINFMDQAYAFAKNNNDNSLGLKICEIQESKISADNCSAWTEMAGKWE
                     TFGNQTRASSARAKADACNKAAADAQREYEANQRKQARKDNFDFSMYAGFYPLPLLTI
                     QTNYWDYGGVLGLGLYNMQIEASYKIINRNLVFFEDLSWQEYDTPDQKVFWDGYRAHI
                     AFKFGDRSRYGEGNYVGPLFEIVERNMTNIRSVVSDESTGAYIGELNFEPKETAYNVM
                     VNFGVHIEENHLMADAFMGLGASYIQFSTGAQGYTTDEYLFSDPLLEYRKETRFGPMV
                     RMGMTIGLSTRN"
     gene            2183..2908
                     /locus_tag="PLM90_08700"
     CDS             2183..2908
                     /locus_tag="PLM90_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89492.1"
                     /translation="MKKFILIVCFFPLAIFAQPTIGYLGTLNVGAAYYQEDDMSVFLP
                     DSTATLSNYGVSWGVGVDYIVNRWLIGASFMYFSQPKTSVDTFDIKTKSNDYRLDIGY
                     MTIVRPKFLFGPVLALGGGFDQVNLMSTENATTAQIAANPGREIHLKQNKFVADLGLQ
                     THYRVASGGEGGGLSLGLQVGYSVSYAIADWRFEDAKISDGPEGLRPMMYARLSIGFP
                     SLESDAGDSEPDNGGGDIYHIPN"
     gene            complement(2964..5717)
                     /locus_tag="PLM90_08705"
     CDS             complement(2964..5717)
                     /locus_tag="PLM90_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ89493.1"
                     /translation="MKLSRSLLIVLLCSLIGFSTLRAQTFKVLNDSCEQLITIDDYPQ
                     ALQACGLAIDKGYSEQVPDSMLMLPRFYLAFALRSLGQREAAVEIMEQLASEILATKG
                     CCDDLVAIYDQLVATYSELNDPKRKLEKMEAIIACETSLHGDSSDFMITYYYLCADDY
                     IITGDYAKSIQYFERAIELYPHFYPDYRNDATYARFLVEYASPLNSVGKTAKAFVLLD
                     TAWAIIQRAPQDETTLNIKNDILQLRVWFYDGIGDAEHAVDAVLQQIEQGEGMNMQTR
                     ANSLVSAALYLINFDIKYGTNVARENNFLDILHNILTVYDSLPPDPIYMSNAYVAIGN
                     YYQVEQIPDSVLHYFRKSSNLIAAAYGKQNVIYLNSLVTQSNYTLLNGDIISAGKILD
                     TVYNLAESFLKSNFLFLSESEQEAVLNDYIPLMNKRNAFYATHLHTFNDVEVALFNSS
                     LFIKGLLLQNITGLRTAVINTGKTDLITLFNEWTNIKTKIAANTVNNKPIPDELTQLA
                     ESLEKKILQSKTLSRTINTSTTVRDISQALEKNSAVIDFMHIISDNDDAYYALVLTSG
                     SHKPQCIRVCVADTLSTILERMPGEREAEYIGRLYTIPDKNFPEDNNYFLGTQLYQLL
                     WEPIRDVLGNVSHLYICADGQLHTIAFHALNDSEGVPLTEHTLITYVNSSRDILHISS
                     DVHPDPVLLVGGLNYSRPNFDPYWRYLPGTLNEIESVESKLNVHNIRFTDYRSDHATE
                     SALIPLNTYYESMHFATHGFAFPAFTDTILPEYGIHFQTSSDPLTRCGLVLSGGDIGW
                     MQQDIQSGIDNILTGNEIANLDLTHTKLVVLSACETSGGDLRGSEGVYGLQRAFRIAG
                     AGALVISLWQVPDQYTTELMQYFYDAYLNGNAPDVALRMAQRNMQKKSQTYYWAAFKC
                     IQG"
     gene            5878..7830
                     /locus_tag="PLM90_08710"
     CDS             5878..7830
                     /locus_tag="PLM90_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMZ89494.1"
                     /translation="MKKHMLTFVAAVCLTVAANAQMGMGMVENPALHNTFRAKKPDAK
                     GYGSEMPKAWSLMAYTPYVKSQGQYGTCASWSSTYSAYTTAWAIKMGMTDRNLITALS
                     FCPYFVYNQVNGDANCSSGNSLEDVLVFMMDNGSKRFYMPVIGCGTGVTPEMTADASQ
                     YKINDAYILYDVQDFPQERTAAAVTKFLTTKAKPDIDAIKSAIASNNPVVFGGFVANS
                     FMQAMGTDHWTPTYEERTNPGQAVLDNHGMHQLHAMTIIGYDDNKYGGAFQIMNSWSS
                     LWGENGYIWVSYDDWALFNYKAFWLDIGFVRLEILTKEGCASGDCNNGYGVMKWNNGQ
                     RYEGHFVNGERSGYGIYTWPDGNAYAGQWKAGKREGEGVLYNPDGSYGTCMYANDVQV
                     SGFGNWTYNNGDRYQGVLNTGYVRNGYGVYTFTDGRVYEGAFKDESFSGLGKMTWPNG
                     DSYMGEWLDNNMHGYGIYTYANGKVKAGKWNFGLLGNGQSYGYAASDGHQANRIQGLF
                     AAVDYATADCVTGDCLNGKGSKKYSSGLVYEGEFKDALEDGYGTWTFTDGSKQTGYFV
                     QGTPAGVFSVTFSGGDKMIGENVKGKVNGYGLYISSSGTMTLEYYSDGTFIREVTPSS
                     TTMQTQLTPEKFGEKPHTDLDVNQSK"
     gene            7833..9503
                     /locus_tag="PLM90_08715"
     CDS             7833..9503
                     /locus_tag="PLM90_08715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMZ89495.1"
                     /translation="MMRNILWRLCLLVMMLSIGNALLYAQTPFEYKYAQDVFNKLIRT
                     INLSSPPKPILEVVPDPNMLATTSGNGKVTIGYQLVNKCRSFGKDSSNALAHVLSHEL
                     THYYQNHFWSEQFGSAYADVGWGEQMQQSGKSLNVMRLYETQADEYGMYYAFSAGYRT
                     LYIGDKVLDSIYTWFHLNEQLPGYPSLTERKQISIEARANIAKLIPVFETGNNCLLLA
                     QMMGGDQQMLLAKTAALQFDDIIARKMQTKEMYNNAAIARLLSVIPMFPDSLQNLQYP
                     FMVETGSILYKSERSRSANGVTDPVMLDAMNTTIDEANTLLNNAVKLDKQFYPAWMNL
                     AILYYLRGAYGSATDALQNASRSMGANNALLWSVDEMRAIIAAAQDDDAEATQLFSSA
                     TTKGSQTATYNYACVYRDASNGLIKIDNTLSAYATDTTEMINGESIFHFMDNMPPNRA
                     GRFDVQNEELVLFTDTTSEYILTYVRANFKPAYSKLRFISSSENPAVKTSKGLRKGDS
                     LQQLIALYGKPSKIFLTGRGEVYAYPAQNIFVHISDEHVSEWTYWWLK"
     gene            complement(9507..10043)
                     /locus_tag="PLM90_08720"
     CDS             complement(9507..10043)
                     /locus_tag="PLM90_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HMZ89496.1"
                     /translation="MIPPVQEAPLGMEENCGLGAEAWGLRGSEPTPCTGSEQVVMKDT
                     DFSPPIKITHATKKPDSATKYAHAGDKQQYVTLTKPQVQEQDSDVGETEERTLHPAAI
                     AGLILTILGGGILSPIGWVGIILSVVALKKIKTNPDIYYGKGLAITSMIYFIISIIAL
                     IALIIFAIYFSGIFLSIP"
     gene            10264..10608
                     /locus_tag="PLM90_08725"
     CDS             10264..10608
                     /locus_tag="PLM90_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HMZ89497.1"
                     /translation="MNIENAQAQMRKGVLEFCILAIISEGEIYPTDVISRLKESNLLV
                     AEGTVYPLLNRLKTMELLSYKWVESNSGPPRKYYQLTDTGRKFLSELDKTWQELVHAV
                     SINTKNIKLTQQ"
     gene            10605..12200
                     /locus_tag="PLM90_08730"
     CDS             10605..12200
                     /locus_tag="PLM90_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMZ89498.1"
                     /translation="MNKLLFVNIGGVVFQIDDTAYRTLDQYLESIRKKYANTAEGEEI
                     IRDIENRIAELFLEKVGERSAITMQYVEEVIALMGAPEDFESASSEQSSTGQQQQYRQ
                     GPSTTRFFRDKEHNVLGGVCSGLAAKFDIDALWLRLAFLIAFFFAGTGLLLYIILWII
                     IPEAKTTAEKLEMRGEKIDIDNIERTVRDGAKQFSQRMNAFGEEVKSTFSSENMEKTR
                     RNTGDFIEKTVNTIAPVGKTIARVVAFGIMLVCMLLIVVVSIELISNWGRHFMDVNFL
                     GSHIMEGSNQAWLMVTCALALFFIPLLGIIVSMVKYLLGIKQRTRFISITLGVLWSIA
                     LFGAIYIAITIGRNFQYEASVNSKLPVVQPANSTMYLQLEMVDGNWPYRGHFDKHGPI
                     KKGDGGPFFEWYEQDSLAFKDISVAFERSVDSNYVVLVNKSARGYDNASAKETAQSFT
                     YNLQQFGDSVIHIPSLIKLQDNEQWREQSVEIIIRVPVNKYVQIDHRLENYLDYNEYI
                     SDLRTPQLFDNKLRMTTAGLQAY"
     gene            12271..12795
                     /locus_tag="PLM90_08735"
     CDS             12271..12795
                     /locus_tag="PLM90_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89499.1"
                     /translation="MQISPAHWHLLVNHFPIILVMTGALFFILGFIMKKPWLHNAALL
                     LTILAALAGFIANQTGEGAEESVEELPGISHQAIHIHEETAETGLIIILITGIVAVIS
                     TLVNMKNKKTGRIFLIITMLATFASSGYMGYVGLTGGEIRHSEIRGDFGRGNTANSNT
                     APAGGEEEAGEDDD"
     gene            12805..13338
                     /locus_tag="PLM90_08740"
     CDS             12805..13338
                     /locus_tag="PLM90_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMZ89500.1"
                     /translation="MQNPLILPVHGVMPEFGENCYIAPNATVVGQVKMGNDCSVWFNA
                     VVRGDVNTIVMGNKVNVQDNAVIHCTYKTAPTTIGNEVSIGHNAIVHGCTIHDHVLVG
                     MGAIVMDHAVVHENVLIAAGSVVLENSILESGFIYGGVPAKKIKPLSPEEFANTISRI
                     SNNYVMYAGWFSGENGD"
BASE COUNT         4047 a         2591 c         2930 g         3799 t
ORIGIN      
        1 tgtagtcaag tccttctcct gccgccattt cggtgacata ggcgttgatt tctctggttt
       61 gttccatact ccagcctttg cgttcggaga ggtgttgatt gatgcttttt cccggttcgg
      121 ttaccaattg tgcgtcgagt aagaagctgc gccaggtgat ttcaacctga ccggcatgcg
      181 caaaaccggc caaagcccgt tcaagacgac gctttcctat atagcagaaa ggacaaacaa
      241 cgtcgctcca tatttcaatt tgtatcatag tgctggtaac agataatcta tgttacaaag
      301 ttcatataaa atgcattgta atatatcttt gatgccatga aacttaccgg ctcctcaaaa
      361 attttaggca agtgctgttt gctgattgca attttcatgc taacggggca tttgaaggcc
      421 caggcaacgt atactgctgc tgatatcgct ttagactctg ctgtgcatca ttataatgcc
      481 tacagaaata cactagatgc atttacaccc gctaatatta ccgatgagaa aataaatctg
      541 gttgaaagta attgcaatat aattgtacgt gaattcgaaa gtataatggc attaaatgca
      601 aaccctcaga gtgaagtagc aaaatatttt catgccatga cgaagtatga atatggtttt
      661 gtgttaggca tggcaggaaa aaatgacaaa gcctatcgca taatgtcgga aattgaagat
      721 tacatgcaga aacaaggcag cttcagtttt ccaaaaaagt atttgtatga cggaaaaaat
      781 tatgttattc aatgggataa ttttgcgccg accttacatg agtatttcac gggtatggcc
      841 gaaatcacca tcaacctcgg aatgaacgag aagaccatgc cctatgcccg cagcgccatg
      901 tcactgcaat atccaaatac cggtgacaat gccgtttggt atcgatatat cgctgcttca
      961 aaatatatgc aatcgtcgaa gaatattgga tattatgcct atgacggatt tgaggtagca
     1021 ttgaattttt tggattggtt atatagcctg gatgccgatt atcaaaagtt ggtaaatgat
     1081 tatgattatc ctcggcaggt tactgcttat aattatattt tgttggttga aaaaggatac
     1141 ccggcaacca atacaggaga gaactggttg aaggcggcaa ctgcttttat gaaatttaat
     1201 gaaagtgctt atgcgggata ttgttttaac agagcggcag atttaaagta tgacaatatc
     1261 aattttatgg atcaggcata tgcatttgcg aaaaataata atgacaacag tttgggattg
     1321 aaaatttgcg aaattcaaga gagcaaaatt agtgctgata actgcagtgc ctggacagaa
     1381 atggcaggga aatgggaaac atttggcaat caaacacggg cgtcatcggc acgcgctaaa
     1441 gcagatgcat gtaataaagc agcagcagat gcccaaaggg aatatgaagc caaccaacgc
     1501 aaacaggcgc gtaaagacaa ctttgatttt agtatgtatg ccggttttta tcctttacca
     1561 ctattgacta ttcaaacaaa ttattgggat tatggtggtg tgcttggatt gggattgtat
     1621 aatatgcaaa ttgaagcaag ttataagatt atcaatcgca acctggtgtt ttttgaggat
     1681 ctttcttggc aggaatatga cacacccgat cagaaagttt tttgggatgg ctatcgggca
     1741 catatagcat tcaaatttgg cgatcgttcg aggtatggtg aaggaaatta tgtgggccct
     1801 ctgtttgaaa tcgtagaacg aaatatgacg aatataagat cggtggtttc tgacgaatct
     1861 accggagcat atattggcga attaaacttc gaacccaaag aaaccgccta taatgtcatg
     1921 gttaattttg gtgtgcatat agaagaaaat catttaatgg cagatgcctt tatgggattg
     1981 ggcgcatcat acattcaatt cagtacagga gcacagggtt atactaccga tgaatatcta
     2041 ttttccgacc cactccttga atacagaaaa gaaaccaggt tcggacccat ggtgcgcatg
     2101 ggaatgacca taggcttatc taccagaaat tgatcgcgct aattgccata caggtcgtat
     2161 ttttattatt gaaatattta tcatgaagaa attcatcctc attgtttgct tttttccgct
     2221 tgcaattttt gcacaaccta ccattggtta tctcggaaca ttgaatgttg gtgccgcgta
     2281 ttatcaagaa gatgatatgt ctgttttcct gcctgacagc actgcaacat taagtaatta
     2341 tggtgtttca tggggagtag gtgtagatta tattgtaaac agatggctaa tcggcgcttc
     2401 atttatgtat ttttcacaac ccaaaaccag cgtcgacact tttgacataa aaacaaagag
     2461 caacgactat cgattggata tcggatacat gacaattgtt cgtccaaaat ttcttttcgg
     2521 tccggttttg gctttgggtg gtggttttga tcaagtgaat ctgatgagta cagaaaatgc
     2581 cacaacagct caaatagcag ctaaccccgg tagagaaatt cacctcaagc aaaacaaatt
     2641 tgtagctgat ctgggtttgc aaacacatta tcgcgtagca tccggaggtg agggtggtgg
     2701 actttctctc ggcttacaag ttggctatag cgtttcttat gccatagcag attggagatt
     2761 tgaggatgcg aaaatatctg acggtcccga aggcttgcga ccaatgatgt atgcgcgctt
     2821 atctatcgga tttccctcac ttgaatctga tgccggtgac agtgagccgg ataatggcgg
     2881 tggcgatatc tatcatatac cgaattaata gttgtgatga ttgcagtcag tttacaacct
     2941 tgtaatcagg cttgattaga aattcaccct tgaatacatt tgaaggccgc ccaataataa
     3001 gtttgtgatt ttttttgcat gttgcgctgt gccattcgca gtgctacatc cggtgcgtta
     3061 ccatttaaat atgcatcata aaaatattgc atcaattcgg tggtatattg atcaggcacc
     3121 tgccataaac tgataacgag ggcaccggct ccggcaattc gaaacgcgcg ttgcaatccg
     3181 tatactcctt cactccccct taaatcgcca ccactggttt cacatgcgct tagcacaaca
     3241 agtttggtat gcgtgaggtc gaggttggct atttcatttc ctgttaaaat attatctata
     3301 cccgattgaa tatcttgctg catccagccg atatcaccac cacttaatac caaaccacaa
     3361 cgcgtcaacg gatctgatga agtttggaaa tgaataccgt attccggtaa aatagtatcc
     3421 gtaaatgcag ggaaagcaaa tccatgtgtt gcgaagtgca tggattcata ataagtatta
     3481 agtggtatca atgcagattc tgttgcatga tcgctgcgat aatcagtaaa gcgaatgttg
     3541 tgtacattga gcttagattc aactgattca atttcgttca atgtgccggg aagatatcgc
     3601 cagtaaggat caaaattggg tcgactatag tttaatcccc ctaccaataa aacaggatcc
     3661 ggatgtacat cacttgatat atgtaatata tcgcgcgaac tatttacata ggtaatcaag
     3721 gtgtgttcag tcaaaggaac gccttcgctg tcattcaaag catgaaacgc gatggtatgt
     3781 aattgaccat cggcacaaat atatagatgg ctgacatttc ctaatacatc acgaatgggt
     3841 tcccaaagca attggtataa ttgtgtgcca aggaaataat tattgtcttc cgggaaattc
     3901 ttgtcgggaa tggtatataa tcgcccaata tactccgcct cgcgttcacc gggcatgcgc
     3961 tccaggatag ttgatagtgt gtctgccaca catacccgaa tgcattgtgg tttatgagat
     4021 ccggatgtta ataccagtgc ataataggca tcatcattgt cggaaataat gtgcatgaaa
     4081 tctataacag cactgttttt ttccaacgcc tgtgatatat cacgtacagt ggttgaggta
     4141 ttaatggtgc gacttaatgt ttttgattgc agaattttct tttccagcga ttcggcaagt
     4201 tgtgtcaact catcgggaat gggtttattg tttaccgtgt ttgccgctat tttagttttg
     4261 atattggtcc actcattgaa taaggtaatt aaatctgttt tcccggtatt gatcactgct
     4321 gttcgcaaac cggtaatatt ttgtagcaat aatcccttta tgaagagcga cgaattgaat
     4381 aatgcaactt ccacatcatt aaaagtatgc agatgtgttg catagaacgc atttcgttta
     4441 ttcattaacg gaatataatc attcaatacc gcttcctgct ccgattcgga tagaaataga
     4501 aaattcgatt ttaaaaatga ttcagcaaga ttatatactg tatcaagaat ttttccggca
     4561 ctaataatat ctccattaag taacgtatag tttgactgtg ttactagact attcagatat
     4621 atcacattct gttttccata agccgccgca ataaggttgg aagatttacg aaaataatgt
     4681 aatacagaat ccggaatttg ttctacctga taataattgc caattgccac ataggcgttg
     4741 ctcatataaa ttggatcagg cggcagagaa tcataaacgg ttaatatatt atgcaatatg
     4801 tcgagaaaat tattttccct ggcaacattt gtaccatatt taatatcaaa attaattaaa
     4861 tacagtgcag cacttaccaa tgagttggcc cgggtttgca tgttcatgcc ttctccttgt
     4921 tctatctgct gtaataccgc atccaccgca tgttccgcat cgccaatacc gtcgtaaaac
     4981 catacgcgta attgtaaaat atcatttttg atgttaagcg tagtttcatc ctgcggcgcg
     5041 cgttgaataa ttgcccatgc cgtatccaac aacacaaacg ctttagctgt ttttcctacg
     5101 ctgtttaatg gtgatgcata ttctacaaga aagcgtgcgt aggtggcatc gttgcgataa
     5161 tccggataga agtgcggata tagttcaatt gcgcgctcaa aatattgaat agatttggcg
     5221 taatcgcccg taataatata atcatctgca cagagataat aataggtaat catgaaatca
     5281 gatgaatcgc cgtgcaaaga tgtttcgcat gcaatgattg cctccatctt ttccagcttt
     5341 cgtttcggat cgttcaattc agaatacgtg gcaaccaatt gatcgtaaat ggcaacaaga
     5401 tcatcacaac atccttttgt agcaagaatt tctgatgcaa gttgttccat tatctcaacg
     5461 gcggcctcgc gttgacccaa tgagcgcaat gcaaaagcca agtaaaatcg cggaagcatg
     5521 agcatagaat ccggtacctg ttcgctgtat cctttgtcaa ttgctaatcc acatgcctgc
     5581 agcgcctgag gataatcatc aatggtaatc aattgctcgc aagagtcatt caatactttg
     5641 aatgtttgtg cgcgcaaagt tgaaaagcca attaagctac acaataaaac aatcagtaaa
     5701 gacctgctca acttcatgat tttcaaagat agtggtgtaa aattatctta ttgatatgga
     5761 aactcgcatc atattgcatc tttatttaaa ttccaaaaga tgcttcaata actacccaag
     5821 aatcatcggg cgtttgcctg tagtacctta tttttatcac tgctaatgca cttacatatg
     5881 aagaaacaca tgttaacctt tgtggctgca gtttgtctaa ctgttgctgc aaacgcccag
     5941 atggggatgg gtatggtcga aaatcccgca ttacacaaca cttttcgtgc taaaaaacct
     6001 gatgctaagg gttacggatc tgaaatgccc aaagcctggt cgctgatggc atacacacct
     6061 tatgtgaaaa gtcagggaca atacggaaca tgcgcatcct ggtcgtcaac ttattctgca
     6121 tacactacag cgtgggcaat taaaatgggt atgactgatc gcaacctaat cacggcactt
     6181 tcattctgcc cttactttgt ttacaatcag gtaaatggcg atgccaattg ctcttccgga
     6241 aattcactgg aagatgtttt ggtattcatg atggacaacg gatcgaagcg attttatatg
     6301 ccggtaattg gctgtggcac gggtgtaacg cctgaaatga cagccgatgc atcgcaatac
     6361 aaaattaatg atgcatatat tttatatgat gtgcaggatt ttccacaaga acgcacagct
     6421 gctgcggtca ccaaattttt aacaacaaaa gcgaaaccgg atatcgatgc aatcaaatct
     6481 gccatcgcat caaataatcc tgttgttttt ggcggattcg tggcaaattc atttatgcag
     6541 gcaatgggca ccgatcattg gacaccaaca tacgaagagc gtacaaatcc aggtcaggcc
     6601 gtactcgaca atcacggtat gcatcaatta catgccatga caattatcgg atatgacgat
     6661 aataaatatg gcggcgcatt tcaaattatg aattcatgga gttcactgtg gggagaaaat
     6721 ggttatatct gggtgtcata cgacgactgg gcattattta attataaagc tttttggctc
     6781 gacatcggat ttgtgcgact cgaaattctc accaaagaag gttgtgcttc cggcgattgt
     6841 aataatggtt atggtgtaat gaaatggaac aacggtcagc gctatgaagg ccactttgta
     6901 aatggtgaac gcagcggtta tggcatttat acatggcctg atggaaatgc ttacgcaggt
     6961 caatggaaag caggtaagcg cgaaggtgaa ggcgtgttgt ataatccgga tggcagctac
     7021 ggtacttgca tgtacgccaa cgatgtgcag gtttccggtt ttggcaactg gacatataat
     7081 aacggcgatc gctatcaagg agtattaaat acagggtatg tgcgcaatgg ctatggtgtt
     7141 tacactttta ccgatggcag agtttatgaa ggcgctttca aggatgaatc tttcagcggc
     7201 ctcggaaaaa tgacttggcc aaatggcgat agttatatgg gcgaatggct cgataataac
     7261 atgcacggct atggtattta tacttatgcc aatggaaaag taaaagccgg aaaatggaat
     7321 ttcggtttgc tcgggaatgg tcagtcatat ggctatgccg caagcgatgg tcatcaggca
     7381 aatagaattc aaggattgtt tgccgctgtc gattacgcca ctgccgattg tgttaccggc
     7441 gattgcctca atggaaaagg cagcaaaaaa tatagcagcg gattagttta tgaaggtgaa
     7501 tttaaagatg cactggaaga tggttacggc acatggacct tcaccgacgg ctctaaacaa
     7561 accggttatt ttgtgcaagg aacacctgca ggtgtgttct ctgtaacatt tagcggcggc
     7621 gataaaatga taggcgaaaa tgtaaaaggc aaagtgaatg gatatggttt atatatttca
     7681 agttcaggaa caatgacttt ggaatattat tccgacggca catttatccg cgaggtaaca
     7741 ccttcatcga caaccatgca aacgcagttg acgcctgaaa aatttggtga aaaacctcat
     7801 accgatcttg atgtaaatca atcgaaataa taatgatgcg aaatattctg tggcgtttgt
     7861 gtttattggt aatgatgttg tcaatcggca atgcattatt gtatgcacaa acgccatttg
     7921 aatataaata tgcacaggat gtgttcaaca aactcattcg caccattaat ttatcatcac
     7981 caccaaaacc aatcctcgaa gttgttccag atcctaatat gttggcaaca acttccggaa
     8041 atggaaaggt taccattggt tatcagctgg taaataaatg cagaagcttc ggcaaggatt
     8101 cgagcaatgc attagctcac gtattgtcgc acgaactcac acattattat caaaatcatt
     8161 tttggtctga acaatttggt tctgcttatg ccgatgttgg ttggggtgaa caaatgcaac
     8221 agtcgggaaa atcgctcaat gtaatgcgac tctatgaaac gcaggcagat gaatatggta
     8281 tgtattatgc tttttccgca ggctatcgca ctttgtatat cggtgataaa gtgttggata
     8341 gtatttatac ctggtttcat ctcaacgagc aattgcccgg atatccaagc ctaactgagc
     8401 gcaaacaaat cagcattgaa gcgcgagcca atattgcaaa actcattccc gttttcgaaa
     8461 caggaaataa ttgtctgctg ctggcacaaa tgatgggcgg cgatcagcaa atgctattgg
     8521 cgaaaactgc tgcattgcaa ttcgatgata tcattgcacg caaaatgcag acaaaggaaa
     8581 tgtataataa tgccgcaatt gccaggttac tgagcgttat tccgatgttt cccgacagtc
     8641 tgcagaattt gcaatatccg ttcatggtgg aaacgggcag tatattatat aaaagtgaac
     8701 gcagcagaag tgcaaacggt gtaacagatc ctgtaatgct ggatgcaatg aatacgacca
     8761 tcgatgaagc caacacatta ttaaataatg cggtcaaact cgataaacag ttttatccgg
     8821 cctggatgaa tcttgcaatc ttgtattatt tgcgcggcgc ttacggctca gccacagatg
     8881 cactgcaaaa tgcatcgcga agcatgggtg caaacaatgc gttattgtgg agtgtggatg
     8941 aaatgcgggc cataatcgct gctgctcagg atgatgatgc cgaagcaacg caattgttta
     9001 gcagcgcaac aacaaaaggc agtcagaccg ccacatataa ttatgcatgt gtttatcgag
     9061 acgcttcaaa cggattgatt aaaattgaca atacattatc ggcatatgca accgatacaa
     9121 cagaaatgat aaatggagaa agcatttttc atttcatgga taatatgccg ccaaacagag
     9181 caggccgctt cgatgtacaa aatgaagagt tggtgttatt taccgatacc acttccgagt
     9241 atatactcac ttacgtgcgc gcaaatttta aaccggcata tagcaaattg cgttttattt
     9301 cgagcagcga aaatccggcg gtaaaaacat ctaaaggttt gcgcaaagga gattctttac
     9361 aacaattgat tgcattatat ggcaagccat ctaaaatatt tctgaccggt cgcggagaag
     9421 tatatgcgta tcctgcacaa aatattttcg tgcatatcag cgatgaacat gtaagcgaat
     9481 ggacctattg gtggttgaaa taatgattag ggaatggata aaaatattcc gctgaaataa
     9541 atcgcgaata ttatcagggc tattaatgca atgatagata taatgaaata tatcatactg
     9601 gttattgcaa gcccttttcc ataatagata tcgggattcg ttttaatttt tttaagggcg
     9661 accacgctca aaatgatgcc cacccagcca atcggagata gaattccccc tcctaaaatg
     9721 gttaaaatga gccctgcaat ggccgcagga tgcaaagtgc gttcctctgt ttcccccaca
     9781 tcagaatcct gctcttgaac ctggggtttc gttaacgtga catactgctg tttatctccg
     9841 gcatgtgcat atttcgtagc tgaatccggt tttttagtgg catgagtgat tttaataggg
     9901 ggagaaaaat cggtatcctt catcacaacc tgctcacttc ccgtgcatgg cgtaggttcg
     9961 ctgcctcgca gtccccaagc ttctgcccct agtccgcaat tttcttccat gccaagcggg
    10021 gcttcctgca caggtggaat catagagcag ctagccgtaa aaataagaag ggctgacagg
    10081 gcacaaaaag ggtacttggg gaccatatat taatatacaa aacccttgcc aaactgattt
    10141 accaaaaaaa tagaaatttt aactcattga ttatcagcac cttaacaaat tataacacca
    10201 agaactaaga aaaacatgct actatgtatt gcataatacc atacgatgca atacctttgc
    10261 cttatgaata tagagaatgc ccaggctcag atgcgaaaag gtgtgctgga gttctgcata
    10321 ctggctatca ttagcgaagg ggagatctac cctacggatg tgatcagtcg cctgaaagaa
    10381 agcaacttgt tagtagcgga gggaacggta tatccactgc tgaacaggtt gaaaacgatg
    10441 gaactattga gctacaaatg ggtggagagt aattcgggac caccgcgcaa atattatcag
    10501 ttaactgata cgggccgcaa atttctatcc gaattagata aaacatggca ggaattggtt
    10561 catgccgttt ctattaacac taaaaacatc aaacttaccc agcaatgaac aagttattat
    10621 ttgtcaatat aggcggtgtt gtatttcaga ttgatgatac ggcatatcgc acccttgatc
    10681 agtatttaga aagcattcga aaaaaatatg cgaatactgc agaaggagaa gaaattatcc
    10741 gcgatatcga aaatcgcata gcagaattat ttctggaaaa agttggcgag cgcagtgcca
    10801 tcaccatgca atatgtggag gaggtgattg cattgatggg tgcaccggaa gatttcgaat
    10861 cggcatcatc tgagcaatct tccaccggac agcaacaaca atatcgccaa gggccatcaa
    10921 ctaccaggtt tttccgcgat aaggaacata atgtattagg aggagtatgt tcgggactcg
    10981 ccgctaaatt tgacatcgat gcgctttggt tgcgattggc gtttttgatt gccttctttt
    11041 ttgcaggaac aggattgttg ttatatatca ttctgtggat catcattccc gaagcaaaaa
    11101 ccacagcaga aaaattggag atgcgcggcg agaaaattga tatcgacaat atcgagcgca
    11161 ccgttcgcga tggagcgaaa caattttcac aacgcatgaa tgcattcggc gaagaagtaa
    11221 aatcaacatt ttcttccgag aacatggaaa aaacgcgtcg taacaccgga gatttcattg
    11281 agaaaacggt gaacaccatc gcacctgttg gaaaaacgat tgcacgtgtg gtagcgtttg
    11341 gaattatgtt ggtatgtatg ctactcattg tagtagtttc tatcgagctg ataagtaact
    11401 ggggacgtca ttttatggat gtgaatttcc tcggcagcca tattatggaa ggatcaaatc
    11461 aggcttggct gatggtaaca tgtgcattgg cattattctt catcccactg ttgggaatca
    11521 tcgtatcgat ggtgaaatat ttgcttggta ttaaacaaag aacaagattt atcagcatta
    11581 cattgggtgt gctgtggagc attgcattat ttggtgcaat ttatattgct atcacaattg
    11641 gtcgcaattt ccaatatgaa gcaagtgtaa acagcaaatt gcctgttgtg caaccggcta
    11701 attcaaccat gtatcttcaa ctcgaaatgg ttgatggcaa ctggccatac cgcggacatt
    11761 tcgacaagca tggtcccatc aaaaaaggcg atggcggtcc gtttttcgaa tggtatgagc
    11821 aagatagcct agcatttaaa gatatcagtg tggcattcga gcgcagtgtc gacagcaatt
    11881 atgtggtgct ggttaataaa tcggcacgcg gttacgacaa tgcctcggca aaagaaacag
    11941 cacagtcatt tacatataac ttgcaacaat ttggtgattc tgtaatacat attccatcgc
    12001 tgatcaaatt gcaagacaat gaacagtggc gtgagcagtc ggttgaaatt attattcgtg
    12061 tacccgttaa taaatatgta caaatagatc atcgtttgga aaattatttg gattacaacg
    12121 aatacatttc agatttgcga acccctcaat tattcgacaa caagctgcgc atgacaaccg
    12181 caggcttaca ggcatattaa ccatcaagca tcaactattg gcaggcattt tttagaaaag
    12241 gaaattagtt ttaactttaa acatcaaatt atgcaaatta gtcctgccca ttggcacctt
    12301 ctggtgaatc attttcccat catcttagtg atgaccggtg ccttgttttt tatccttggt
    12361 tttatcatga aaaaaccatg gctgcacaac gctgcgttat tattaaccat attagcggcg
    12421 cttgccggct tcattgccaa tcaaaccgga gaaggtgcgg aagaatctgt agaagagctg
    12481 cccggaattt cacaccaggc aattcatatt catgaagaaa cagctgaaac cggattaata
    12541 attattctaa ttacaggtat tgttgcagtg atcagcacgc ttgtaaatat gaagaataaa
    12601 aaaaccggtc gcatatttct catcatcact atgctggcaa ccttcgcgag ttcgggttat
    12661 atgggttatg tgggattgac cggcggtgaa attcgtcaca gcgaaattcg cggcgatttc
    12721 ggacgaggaa atacagccaa ctcaaatact gccccggcag gtggtgaaga agaagccggt
    12781 gaagatgacg attaaacata taccatgcag aatcctttga tacttcctgt gcacggcgtc
    12841 atgcctgaat ttggtgagaa ttgttatatc gctcctaatg ccaccgtagt tggtcaggta
    12901 aaaatgggca acgattgcag tgtatggttt aatgcggttg ttcgtggtga tgtaaatacc
    12961 atagtaatgg gaaataaagt gaatgtgcag gataatgctg ttatacattg cacttacaaa
    13021 acagcaccaa caaccatcgg taatgaagta tctatcggtc acaacgcaat cgttcacggc
    13081 tgcaccatcc acgatcatgt gctggtgggc atgggagcga tagtaatgga tcacgctgtt
    13141 gtgcacgaaa atgtattaat agcagctggc agtgtggttt tagaaaatag tattcttgaa
    13201 agtggcttta tctacggtgg tgttcctgca aaaaaaatca aaccactcag tccggaagaa
    13261 tttgcgaata cgatttcgcg tatctcaaat aattatgtga tgtatgcggg gtggttttcg
    13321 ggggaaaatg gtgattagca aatttgagaa ttagcagatt agctaat
//
LOCUS       DAOQOU010000088        13281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2404_length_13281_cov_4.971647, whole genome shotgun sequence.
ACCESSION   DAOQOU010000088 DAOQOU010000000
VERSION     DAOQOU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_13281_cov_4.971647"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="PLM90_08745"
     CDS             <1..1941
                     /locus_tag="PLM90_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89501.1"
                     /translation="FAKQMAPWGAYAKASSPLDQSYQLSGGWVKKIGKVGEFGAIGAL
                     TYNNSNVSSFIKRNDFDNGTDSLYAYQDDQFKNNILWGGLLNLSWKLNDNTRFSFKNS
                     YNINSSDITTMRSGVNFSRAQYVQNEYYEFNSNELFSTQISGDHYLTGSQVKLKWSLG
                     MNTMKRDQPNYRSVNYAKNITPSFDGDTLFTISPSPFASPENLGIFYSYMDEQTYTAG
                     LDITKPFKILGTKQSLGFGGSYLTKARSFDARIMGVATANDIFFNPDYFNIVSLPIGE
                     LLQPANFSDSLFYLDEITNPSDAYTATQENKAIYLMIDNQIANKLRVVWGARAEFFNQ
                     TLQSFAYGFSSTPDTVEVDTKETDSVGLKFDFLPSINITYGVTAKTNIRVSAYKTVAR
                     PELRELAPFGYYDIETNSSIVGNPSLKATNIYNADVKAEHFFGAGQVISGGVFYKKFY
                     DPIGQRFFFGTVRELKPINDSLATVVGAEFELRKNLGFISPKTKWLEQFTFNTNLAVL
                     NSTTVLHIADTAIAGTAERDMQGQSDYVINAGFTYIQPEKGYSCTLLFNQIGRRISEY
                     GNAQYDDIYENPRPLVDAQVSVPFNKGKGNVKLNMSDILNKDAVFYQDVDGDGNYVAE
                     KDNAIRVIDNGAKISLSVNYTF"
     gene            1969..3831
                     /locus_tag="PLM90_08750"
     CDS             1969..3831
                     /locus_tag="PLM90_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ89502.1"
                     /translation="MKKLLFTLLVSLGCIGAANAQLPTATLTGEISGITRNLVNDTVY
                     ILNGFVYVEDGATLNIEAGTIIRGDKDTKGSLIVTKGSKIYANGTADQPIVFTSNQPE
                     GTRNYGDWGGLIILGNAPINVPGGTAVIEGGVDTPEGDGVYGGLDPLDNSGIIKYVRI
                     EYPGIAFVPGNEINGLTCGGVGAGTVIDHVMVSRSGDDAFEFFGGTVTAKYLIAHNAL
                     DDDLDTDFGFQGKIQFAVVKRDPNFADVSGSNGFESDNDATGSTNAPQTNPTFSNITV
                     VGPIQNAGDVINVNYKRGAHLRRNTSTDIFNSVIIGYPSGIMIDGALCEASATDNNLK
                     VQNTIIAGHNNDFEVAAGSTFDANAWFTTAAYNNTIYDLSSDVMLTDAYNATSPNFLP
                     MAGSPLLSGSSFAAPELAGFTNTTYRGAFGDLDWTSCWANWDPQNTNYGEVAGTIAPA
                     IASFSYANSDLTVSFSNTSSNAVSYIWDFGDETTTDDVSTEANPSYTYPALGVYTVTL
                     TATGTCADQNTTTNNLDLSVAITDASALQSIMLYPNPASDVATLVINATEDMQGVVTI
                     LDMAGNMVMPAVEQHILSGKTTINLATADLASGMYMVNILAQGAQQTLPLLIAK"
     gene            complement(3884..4303)
                     /locus_tag="PLM90_08755"
     CDS             complement(3884..4303)
                     /locus_tag="PLM90_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89503.1"
                     /translation="MQFLHDRPHFVIVCEPGKGIAEMLTGYLHYYAGSSAPVSWYPAQ
                     STIPTLVEQVLHEDGIHNWHPFNDENSGEPVVICINRVELPVTVKTILQRYQSEQITL
                     DLPTETSINIHQLRALREEIRQCAIMIMGKFSTTVMQ"
     gene            4388..5359
                     /locus_tag="PLM90_08760"
     CDS             4388..5359
                     /locus_tag="PLM90_08760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HMZ89504.1"
                     /translation="MSDMKWRTEVMPDPLQTKIAVNAKCIFAGSCFAENLGAFLQQYK
                     FNVLVNPTGIIFNPIAIANALETGASNTIPSQDRLFNHVGLWRHPDVHSNLAHPNRDS
                     AWNLIENAYNALHQHFTQSSHIAVTFGTAWVYNERSSGKIVANNHKLPAAHFTKQLLS
                     VNEIVNTWQPFIAQFPEKQWIFTVSPVRHTRDGMTQNSRSKAILIESVNQLAENNNNV
                     YYFPAYEIMMDDLRDYRFYEQDLIHPNQQALQYIRSLFVQTCLDDHAAKWMAAFEPII
                     AARNHRPLFPDSQEHAAFKKSILLKLESLKKEYPIMHIAEELDYFAS"
     gene            complement(5360..6835)
                     /gene="rmuC"
                     /locus_tag="PLM90_08765"
     CDS             complement(5360..6835)
                     /gene="rmuC"
                     /locus_tag="PLM90_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HMZ89505.1"
                     /translation="MELIFLIIGIVIGAVAAWLIRKYMSERDMVSVKELDAKQSALLQ
                     AENTIKYLQEKQNDLQASVAQAMQELRSAQTEKEQVQIQLATSNTSLQQLSQQLTETK
                     NQAEQFANEINELKNFMIKVRGENEVLKEKNATIKKEIEELGEKSTMHFRNLANEILE
                     DKSKRFTEENQKNIKLILDPLNQDIRDFKKKVEDTYEKENKQRFSLEEKIRDLVSLNQ
                     RISDEANNLTKALKGQRKTQGDWGEMILENILQQSGLTEGREYAKQTTLRDESGNTLI
                     NEDGRRMQPDFIIYYPDKREVIIDSKVSLSAYERFSSADDDEAQKKALDEHVAAVYHH
                     IDELSKRSYQDFAQSLDFVMMFVPIEPAYLVAMQHDRDLWNYAYKKRILLISPTNLIA
                     AVKLVSEIWKKDEQNKNAIKIAERGAALYDKFVGFVENLQTIGDHIARSQRSYDDAMK
                     QLKDGSGNLIGQAEKLRKLGVSAKKTLPGTIIDESDVDDDQ"
     gene            complement(6912..6988)
                     /locus_tag="PLM90_08770"
     tRNA            complement(6912..6988)
                     /locus_tag="PLM90_08770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6952..6954),aa:Met,seq:cat)
     gene            complement(7075..7932)
                     /locus_tag="PLM90_08775"
     CDS             complement(7075..7932)
                     /locus_tag="PLM90_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ89506.1"
                     /translation="MQIYSVHAGLFKLDGGAMFGVVPKKIWNKLVPADDNNLCTWAMR
                     CMVVVGGGHTVLIDCGMGDKQDEKFFGHYEPHGPYSLEASLKTHGITPGDITDVFLTH
                     LHFDHCGGAVKKNAKGKLIPAFENARYWSNEKHWAWAMQPNAREKASFLKENFQPLQQ
                     HGVLHFIEENEELFPGFNTVWTYGHTEAMMLPLIDYKGSKLLYCADLLPSHAHIPLPY
                     VMGYDVRPLVTIKEKEAIIKEAHDKKWWLFFEHDAEHACCNLITTEKGIRPDTFSTPE
                     DIPVLVKKR"
     gene            complement(7973..8737)
                     /locus_tag="PLM90_08780"
     CDS             complement(7973..8737)
                     /locus_tag="PLM90_08780"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMZ89507.1"
                     /translation="MKQLFWKWFIAVDRRLKFPSLKAGETGVQIGFDMSAPVTSDLFL
                     MVENVGPAGNVIGIDPDPRNIAAAHLIVARQRYPVTLIHRAVFNHEGKMGLLLGEKAS
                     WNQLTNVPIDDTVSFNGEEVVVEMDTLDNIFHQFNIDTNTIGHINITINGAEYFALLG
                     MHQILQKKDLALTVVAGRYDDSGMIDGEPDHEKIMALLKSYGFKVTFRRIHQMFWWGF
                     VINTLMKRRWIYGKDNYGIVMAAKGSKRIPWFQSFS"
     gene            complement(8748..9239)
                     /gene="ribH"
                     /locus_tag="PLM90_08785"
     CDS             complement(8748..9239)
                     /gene="ribH"
                     /locus_tag="PLM90_08785"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMZ89508.1"
                     /translation="MATGKQHLTPQQEAAIPSGDQFRIGIVVSEWNTAITEKLYQGCY
                     HTLTHYKTPSEQIITVYVPGSFELPQAAQLLLESQELDAVICLGCIIKGETSHDQHIA
                     QAVAHGVIQVGLDYSTPVIFGVLTTNDEQQAIDRAGGKHGNKGEEAAITALRMAALRE
                     KLI"
     gene            complement(9239..9931)
                     /locus_tag="PLM90_08790"
     CDS             complement(9239..9931)
                     /locus_tag="PLM90_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89509.1"
                     /translation="MSEVNKSATDTTLDKGTYEHVENFFLKNRNNILIALGVIVLAIG
                     GYFAYKQLYMSPRVATAQNNIAGAQNYFAKDSFDLALNGDGAVMGFLAIAENYGNTPS
                     GNMAHYYAGVCYMRKAELDNAIKHLEDFNPTTNEIAGEKYVLLGHAYADKKDYKKAID
                     MYKKAGDEAENNLQSPAFYKFAGDLMSEQNDLKGALEMYRKIKQLYPLSQEGQNIDLD
                     ITYAENKLGIKE"
     gene            complement(9941..10936)
                     /gene="pdhA"
                     /locus_tag="PLM90_08795"
     CDS             complement(9941..10936)
                     /gene="pdhA"
                     /locus_tag="PLM90_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HMZ89510.1"
                     /translation="MDRSKITKETYLNWYELMYKMRRFEEKAGQLYGQQKIRGFCHLY
                     IGQEAVAAGTISAIRHEDPIITAYRDHGLAIAKGISCNAAMAELFGKVTGVTQGKGGS
                     MHFFSKENYFFGGHGIVGAQIPLGAGIGFAEQFKGTGNVCLCYFGDGAVRQGSVHETF
                     NMAMLWKIPVIFICENNGYAMGTSLERTTNQLDMYKIGLAYDMPSYPVDGMQPETVHA
                     AIYEAAEKARNGEGPTFLEIRTYRYRGHSMSDPGKYRTKEEVEEYKLRDPLETTAQKI
                     LDKKYATQKELEDIQARIEEEIDACVKFADESPWPDDAAVYEDIYVQQDYPFLKD"
     gene            10997..12100
                     /gene="recF"
                     /locus_tag="PLM90_08800"
     CDS             10997..12100
                     /gene="recF"
                     /locus_tag="PLM90_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HMZ89511.1"
                     /translation="MLLRSIRLLHFKNYASAEAVFSHAFTLINGLNGSGKTNLIDAVY
                     YLCLCRSYFTRSDSSVLQHDAPYFRIDGTFSNPEGSLLITCKYLTAKKEVLRNEVPYD
                     RISDHIGNFPVAMIAPDDINIINEGSEERRRFLDAAISQLDREYLNNLILYNKYLAQR
                     NALFKQALGGGIRPDATLVGVLNHSLALHGNAIYRARRAYLEAFTPIFRDMYSRISGG
                     TEDADISYKSQLEEHDHLHLLLHSERDDRAAGRTTSGIHKDDLYLSVKQYPVRDTGSQ
                     GQIKSFLIALKLAQCYMMFRSEGKQSILLLDDIFEKLDKQRLEVLFNMLQDEVFCQVF
                     ITDADTKRSMEFCKSHLPDFGHIHVNNGKIEYL"
     gene            12107..12397
                     /locus_tag="PLM90_08805"
     CDS             12107..12397
                     /locus_tag="PLM90_08805"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HMZ89512.1"
                     /translation="MKKDNQLSIGDAIKQLFHSIRIEDKVAEVRIKELWPSIMGASIN
                     RYTGEIYFNKGILTIYINSAPLKQDLQFSKQEIIERVNEELQENLVREVIIR"
     gene            12453..>13281
                     /locus_tag="PLM90_08810"
     CDS             12453..>13281
                     /locus_tag="PLM90_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89513.1"
                     /translation="MELFVRGRWGIWLFNLFGSAGTKWREDVTYDANGNILTYFRNGN
                     TSTMDNLSYVYNTGNNQLKRVDDAVSSGTYSVDIDDQSGTNYEYDALGNLVKDVAEEI
                     DTILWTLTGKVDSISRTGASTKDELSFLYDPMGNRVGKIVYEDDGDILKTWYIRDAQG
                     NIMSTYTEKEDSLWWTEVDLYGSSRLGVIYPDSLVYPYTYTMPGDTFSFFNIAGNRRY
                     EITNHLGNVLATITDRRIPQEVGTPNGIAEYYLADIASVQDYYPFGMVTPGRSYSAGS
                     "
BASE COUNT         3770 a         2909 c         2697 g         3905 t
ORIGIN      
        1 tttgctaaac aaatggcacc ttggggtgca tatgccaaag cttcatctcc attagatcag
       61 tcttatcaac tgtcaggcgg atgggtgaag aagattggta aggtaggtga atttggggct
      121 attggcgcac tgacctataa caatagtaat gtctcaagtt ttatcaaacg caatgacttt
      181 gataacggaa ccgatagcct ttatgcttat caggatgatc agtttaaaaa caatatttta
      241 tggggtggat tactgaacct tagttggaaa ttaaatgaca acacgcgatt ctcattcaaa
      301 aactcctata atatcaattc tagcgatatc actaccatgc gcagtggtgt gaatttcagt
      361 cgcgcgcaat acgttcaaaa cgaatattac gaattcaact ctaacgaatt attcagtacg
      421 caaataagtg gtgatcacta tttgaccggc tcacaagtta aattgaaatg gtctttgggt
      481 atgaatacca tgaaacgcga tcagcctaat taccgttcgg tgaattatgc aaaaaatatt
      541 acaccaagct tcgatggtga taccttattc accatttcac cttccccatt tgcatcgcct
      601 gaaaatcttg gaatatttta ttcctacatg gatgagcaaa cttatactgc aggactggat
      661 atcactaaac cttttaaaat tcttggcacg aaacagtctt taggttttgg tggcagctat
      721 ttaactaaag ctcgttcttt cgatgcccgt ataatgggag tggctacggc taacgatatc
      781 ttttttaatc ctgattattt caatatcgtg tcattgccta ttggcgaatt gttgcaacct
      841 gcaaattttt ctgatagcct gttttatctc gatgaaatta ccaatccttc agatgcctat
      901 accgcaacgc aggaaaataa ggctatttac ctgatgatcg ataatcaaat agcaaataaa
      961 ttgcgtgtgg tatggggagc aagagctgag ttctttaatc agaccttgca atcctttgca
     1021 tatggttttt catccactcc tgatacggta gaagttgata ctaaagaaac tgacagcgta
     1081 ggtttgaaat ttgatttcct gccatccata aatattacat acggcgtaac tgcaaaaacc
     1141 aatatcaggg taagtgccta taaaacagtt gctcgtcctg aactgcgtga actcgcgcca
     1201 ttcggttatt acgatattga aacaaactca agcatcgttg gaaacccatc gttgaaggca
     1261 accaacattt ataatgccga tgttaaggcg gaacactttt tcggggcagg acaagtgatt
     1321 tccggaggtg tattctataa gaaattttat gatccaatcg gacagcgatt cttcttcgga
     1381 actgttcgcg aattgaaacc aataaatgat tcactcgcta cagttgtcgg cgctgagttc
     1441 gaacttcgta aaaaccttgg atttatctca cctaaaacta aatggttaga gcaatttaca
     1501 tttaatacca atctggcagt attgaattca actacagtgt tgcacattgc ggatacagct
     1561 attgccggta cagcagaacg cgatatgcaa ggacaatctg actatgtaat caacgccgga
     1621 tttacttata tccaacctga aaaaggttac tcatgcacct tattattcaa tcaaatagga
     1681 agacgaattt ccgaatatgg aaatgcacag tatgacgaca tttatgaaaa cccaagaccc
     1741 ttagtagatg cacaggtttc agttcctttc aataaaggaa aaggaaatgt aaaactcaat
     1801 atgagcgata tcctcaacaa ggatgctgtt ttctatcagg atgttgatgg cgatggcaat
     1861 tatgttgcgg agaaggataa cgctatccgt gtgatcgaca acggagcaaa aatttcatta
     1921 tctgtcaatt atacttttta atacaagaac tacaaacaaa tcaatgttat gaaaaaactg
     1981 ttatttacat tattggttag ccttggatgt attggtgctg caaatgccca attgccaact
     2041 gcaactttaa ccggcgaaat ttccggtatt acacgtaatc ttgtcaatga tacggtgtat
     2101 atattaaatg gctttgtata cgtggaagat ggcgccactc tgaatataga agccggaacc
     2161 attatccgtg gcgataaaga cactaagggg tcgctgatcg ttactaaagg ttctaaaatt
     2221 tatgctaatg gaactgctga tcaaccaatc gttttcacca gtaatcaacc cgaaggcact
     2281 cgtaattacg gcgactgggg tggtttgatt attctcggaa atgcacccat caatgttccc
     2341 ggtggtactg ccgttatcga aggtggtgta gatacgcctg aaggggatgg cgtttatggc
     2401 ggactcgatc cattagataa ttcaggtatt attaaatatg tgcgcatcga atatccgggt
     2461 attgcctttg ttcccggtaa tgagatcaat ggtcttacct gtggtggtgt gggtgccgga
     2521 actgttattg atcacgtaat ggtgagcaga tcaggtgatg atgcatttga attttttgga
     2581 ggtaccgtaa cagctaagta tttgattgcg cataatgcct tggatgatga tttagatact
     2641 gactttggat tccagggtaa aattcaattt gcggtagtaa aacgcgatcc aaactttgcc
     2701 gatgtttcag gttctaacgg attcgaaagc gataatgatg ctaccggatc aaccaatgca
     2761 ccgcaaacaa atcctacatt cagcaatatt accgttgttg gaccaatcca aaacgccggt
     2821 gatgttatta atgtgaacta taagcgaggt gctcatttac gcagaaatac ttctactgac
     2881 atttttaact cagtaatcat aggttatcct tcaggtataa tgattgatgg tgccttgtgc
     2941 gaagctagtg ctacagataa taacctgaaa gtgcaaaata ccattatcgc aggtcataat
     3001 aatgattttg aagtagctgc cggaagtaca ttcgatgcaa atgcatggtt tactactgca
     3061 gcatacaaca atactattta cgacttgagc agcgatgtaa tgcttacaga tgcttataat
     3121 gctacatctc cgaatttcct tcctatggcc ggttcgcctt tgttgagtgg ctcatcattt
     3181 gctgcgcctg aattagcagg tttcacaaat acaacctatc gtggtgcttt tggcgatctc
     3241 gactggactt catgctgggc aaactgggat ccgcaaaata cgaattatgg cgaagttgcc
     3301 ggcaccattg ctcctgctat cgcatctttc tcgtatgcga acagcgatct gactgtatca
     3361 ttcagcaata cttccagcaa cgctgtaagc tatatctggg attttggcga tgaaacaact
     3421 actgatgatg ttagcacaga agcaaaccca tcttatacct atcctgcact tggtgtatac
     3481 accgttacgt taacagctac cggtacttgt gcagatcaaa ataccacaac aaataattta
     3541 gatctttcag ttgccattac agatgcttct gctttgcagt cgatcatgtt atatccaaat
     3601 ccggcatctg atgttgcaac gttagtgatt aatgcaactg aggatatgca aggtgttgta
     3661 accattttgg atatggccgg aaatatggtg atgcctgcag ttgagcaaca tattctatca
     3721 ggtaaaacaa ccataaatct agctactgct gatctcgcat caggcatgta tatggtgaac
     3781 atacttgctc agggagcaca acaaacattg ccattactca tagctaaata aataagaaag
     3841 ttgcaattag gtaagaggct gcagggaaac cgggcctctt tttttattgc attactgttg
     3901 tcgaaaattt tcccatgatc ataattgcgc attgccggat ttcttcacgt aatgctctca
     3961 attgatgtat gttgatggaa gtttcagtag gtaaatcaag cgttatctgt tccgattggt
     4021 atcgctgtaa tattgtttta acagtaacag gtaattcaac tctgttgatg caaataacta
     4081 caggttcgcc tgaattttca tcattaaacg gatgccagtt atgaatgcca tcctcatgta
     4141 aaacctgttc taccaatgtt ggaatggtcg attgcgcagg ataccaggat acaggtgcag
     4201 atgaacccgc ataataatgc aaatatcccg taagcatctc agcaatgcct ttaccgggtt
     4261 cacaaacgat cacaaaatgt ggtctgtcat gtaaaaattg cattttctta attatgaatt
     4321 aactttgaat gccgatatct gtagtaaata taaattagtg taatcaaatt gcaaattatt
     4381 tatcgcaatg tctgatatga aatggagaac agaagtgatg cctgatcctc tgcaaacgaa
     4441 aattgcggtt aatgccaaat gtatttttgc aggttcttgt tttgctgaaa acttaggtgc
     4501 atttcttcaa cagtacaaat ttaatgtgct ggtaaatcca acaggcatta tttttaatcc
     4561 aattgcaata gcaaatgcac ttgaaacagg cgcctcaaat actattcctt cgcaggatag
     4621 attgtttaat catgtaggtt tgtggcgaca tccggatgtg cactccaatc tcgcgcaccc
     4681 gaaccgtgat tcggcatgga atttgataga gaatgcatat aacgctttac atcagcactt
     4741 tacgcaatcc tcccatattg ccgtcacctt tggaacagct tgggtttata atgaacgcag
     4801 ctcagggaaa atcgtggcaa ataatcataa actacccgct gcacatttta ctaagcaatt
     4861 attatcggtg aatgagattg taaatacttg gcagccattc atcgcacagt tccctgagaa
     4921 gcaatggata tttacagtca gcccggttcg acatacacgt gatggtatga cgcaaaattc
     4981 tcgtagtaaa gcaatattaa tagaaagcgt taaccaactt gcagagaata acaataatgt
     5041 ctattatttc ccggcatacg aaatcatgat ggatgatttg cgcgattatc ggttttatga
     5101 acaagacctt attcacccta accaacaggc attgcaatat attcgatcct tatttgttca
     5161 gacttgttta gatgatcatg ccgctaaatg gatggctgct tttgaaccaa ttatcgcagc
     5221 gcgtaatcat cgccctttgt ttccggattc tcaagagcat gcagcattta aaaaaagtat
     5281 attgctgaaa ttggaatcgc ttaagaaaga atatccaata atgcatattg ccgaagagct
     5341 tgattatttt gcttcctgat tattgatcgt cgtccacgtc actttcatca ataatagttc
     5401 ccggtagtgt tttcttcgcg ctaacaccca gtttgcgcag tttctccgcc tgcccgataa
     5461 ggttacctga tccgtctttc agctgtttca tcgcatcatc ataactgcgt tgcgatctgg
     5521 cgatatggtc gccaatggtt tgcagatttt cgacaaagcc aacaaattta tcatacaagg
     5581 cagcgcctcg ctcggcaatt tttatagcat ttttgttttg ctcgtccttt ttccagattt
     5641 cactcaccaa cttaacagcc gcaatcaaat tagtagggga gatgagtaag atccgttttt
     5701 tataggcata attccacaaa tcgcgatcat gctgcatggc aaccaggtag gcaggttcta
     5761 ttggcacaaa catcatcaca aaatccaggc tttgcgcaaa atcctgataa ctgcgtttac
     5821 ttaattcatc gatatgatga taaactgcag caacatgttc atcaagcgct tttttctgtg
     5881 cctcatcatc atctgcagaa ctgaatcgct cataagcact caaagaaact ttgctgtcga
     5941 tgatcacctc gcgtttgtcg gggtaataaa tgatgaaatc cggttgcatg cggcgaccat
     6001 cttcgttgat cagcgtattg ccgctttcat cgcgcaaggt tgtctgtttg gcatattctc
     6061 gtccttcagt taatccgctt tgttgcagaa tattttcaag gatcatctca ccccagtcgc
     6121 cttgtgtttt tcgttgacct tttagcgcct tagtaagatt attcgcctca tcgctgatgc
     6181 gctgatttaa gctgacgagg tcgcggattt tttcttccaa agaaaatcgt tgtttgttct
     6241 ctttctcata agtgtcctcc accttcttct taaaatcacg gatatcctga tttaaaggat
     6301 caagaatgag tttgatgttt ttctggtttt cctcggtaaa tcgcttgctt ttatcttcca
     6361 aaatctcatt tgcaagattc ctgaaatgca tggttgattt ttctcccagc tcttcaatct
     6421 cctttttaat ggtggcattc ttttccttca acacttcatt ttccccacgc accttgatca
     6481 tgaaattttt caattcatta atctcatttg caaattgttc agcctgattt tttgtttccg
     6541 ttaattgctg actaagttgc tgtaaagaag tattcgatgt tgctaattga atttgcacct
     6601 gttccttttc cgtttgagcg ctgcgcaatt cctgcatagc ctgtgcaacg gaagcttgca
     6661 gatcattttg tttctcctgc aaatatttta tggtattttc tgcctgcaac agcgcacttt
     6721 gtttcgcatc caactccttc acagatacca tatcgcgctc cgacatgtat tttctaatca
     6781 gccaggcagc aacggcgcca ataactattc cgataataag aaatatcaat tccatacact
     6841 aaacaagggc ttgaagttac taaacaatta cttcgcctga ggcaataaaa aaagcaaccc
     6901 gaaggttgct atggtagcgt gggggaggct cgaactcccg acctcagcat tatgagtgct
     6961 gcgctctagc caactgagct accacgccgc gtgcccgctt tacgcctgaa acagcataaa
     7021 ttgatgcgtt gcgggtgcaa aggtagcata tttatcgatg ttttaaaaac cgtatcagcg
     7081 ctttttaacc agcaccggaa tgtcctccgg cgtgctgaaa gtatcaggtc tgatgccctt
     7141 ttccgtagtt atcagattgc aacaggcatg ttcggcatca tgctcgaaga acaaccacca
     7201 tttcttgtca tgcgcttctt taataatggc ttctttttct tttatcgtta ccaacggcct
     7261 tacatcataa cccattacgt agggtagggg aatatgtgca tgacttggaa gtaaatcggc
     7321 acaatacaac aacttgctgc ctttatagtc aatcaatggc aacatcatcg cctcagtatg
     7381 accataagtc cacaccgtat tgaagccggg aaataactcc tcattttctt caataaaatg
     7441 taaaacgcca tgttgctgca atggctgaaa attctctttc agaaaactgg ctttctctct
     7501 tgcattaggc tgcatcgccc atgcccagtg cttttcgttt gaccaatatc gcgcattttc
     7561 aaatgcaggt atcaattttc ctttcgcatt ctttttcacc gctccaccac aatgatcgaa
     7621 atgaagatgc gtgagaaata catccgtaat atcgccgggc gtgataccat gggtttttaa
     7681 tgatgcctcc aatgaataag gaccatgcgg ctcgtaatga ccaaaaaact tctcatcctg
     7741 cttatcgccc atgccacagt ctatcagcac ggtatgtccg ccgccaacca ccaccataca
     7801 gcgcatggcc caggtgcaga gattattgtc atctgcagga accaatttat tccatatttt
     7861 ctttggaaca accccaaaca tggcgccacc atcgagcttg aacaagccgg catgaacaga
     7921 atagatttgc ataaggctaa agtagtgatt atcagattaa tatgctacgc attcagctaa
     7981 aagactggaa ccagggtatg cgtttgcttc cttttgcagc cataacaatg ccgtagttgt
     8041 ctttgccgta gatccacctg cgcttcatca gggtattaat cacaaatccc caccagaaca
     8101 tttggtggat gcgacggaat gttactttaa atccatacga cttcaacaat gccatgattt
     8161 tctcatgatc aggttccccg tcaatcattc ctgaatcatc atatcttccc gccaccacgg
     8221 ttaatgctaa atccttcttc tgtaaaatct gatgcatgcc aagtaaggca aaatattcgg
     8281 cgccattgat cgtgatattg atatgcccga tagtgttggt gtcaatatta aattgatgaa
     8341 atatattatc aagtgtatcc atttccacta ccacttcttc gccattaaaa gaaacggtgt
     8401 catcgatagg cacatttgtt aattgattcc aacttgcctt ttcacccaat aataatccca
     8461 tcttgccctc gtgattaaaa actgctcgat gaataagtgt aacaggatat cgctgacgcg
     8521 caacaattaa atgtgcagct gcaatattgc gaggatcggg atctatgcca ataacatttc
     8581 ctgcagggcc aacattttct accatcaaga ataaatctga tgttacgggt gcactcatgt
     8641 caaagccaat ctgaacgcca gtttcgcctg ctttaaggga tggaaatttc aatcgcctgt
     8701 caactgcgat aaaccatttc caaaacaatt gtttcatgcg aatcttttca gatgagtttc
     8761 tcgcgaagcg ctgccatgcg caatgcagtt attgctgctt cttcgccctt attgccgtgc
     8821 tttccgccgg cgcgatctat tgcctgctgt tcatcattgg tagtcaatac accaaatatt
     8881 acaggcgttg aataatcaag tccaacctga ataactccat gtgctactgc ctgtgcaata
     8941 tgttgatcgt gtgaagtttc tcctttaatg atacatccca agcaaataac tgcatccaat
     9001 tcctgcgatt caagcaataa ttgtgcagcc tgaggtaatt caaaactacc cggaacataa
     9061 acagtgatta tttgctccga aggcgtttta taatgcgtca gcgtatgata gcaaccctga
     9121 tataatttct ctgtgatagc ggtattccat tctgaaacga caataccgat tcgaaattga
     9181 tcacccgaag ggatagctgc ttcctgctgt ggtgtaagat gctgcttgcc tgtggccatt
     9241 attctttaat accgagtttg ttttcggcat aagtaatgtc gagatcaata ttctgacctt
     9301 cctgagaaag cggatataat tgtttgatct tgcgatacat ttccaatgca cctttcaaat
     9361 cgttctgctc cgacatcagg tcgcctgcga atttgtagaa tgcaggactc tgaagattat
     9421 tttctgcttc atcacctgct tttttataca tgtcgatagc ctttttgtag tcttttttat
     9481 cggcatacgc atgacctaac aatacatatt tttcaccggc aatctcgttg gttgtcgggt
     9541 tgaaatcttc cagatgttta atggcattgt ccaattcagc cttgcgcata taacaaacgc
     9601 cggcataata atgtgccata ttgccgcttg gtgtattacc gtaattttct gcaatggcaa
     9661 gaaatcccat cacagcacca tcgccattca atgcaagatc aaatgaatct tttgcgaaat
     9721 aattctgagc acctgcaata ttgttttgtg ccgttgcaac gcgtggcgac atatataatt
     9781 gcttgtaagc aaaataaccg ccaatagcca acacaattac acccaaagca atcaggatgt
     9841 tgttgcgatt tttgaggaag aaattttcca cgtgctcata agtaccttta tcgagtgtgg
     9901 tgtcggttgc ggatttgtta acttctgaca tatcgaaata ttaatctttt aaaaaggggt
     9961 aatcttgttg aacgtaaata tcttcataca ctgcagcatc atccggccat ggcgattcat
    10021 cggcgaattt cacgcatgcg tcaatctctt cttcaatgcg tgcctgaata tcttcaagct
    10081 ctttctgcgt agcgtatttt ttgtctaata ttttttgtgc tgtcgtttcc agcggatcgc
    10141 gcagcttgta ttcttctact tcttcttttg tgcggtattt tcccgggtca ctcatagaat
    10201 gtccgcgata acgataagtg cggatttcca aaaatgtagg accctcacca ttgcgcgctt
    10261 tttctgccgc ttcgtatatc gctgcatgaa cggtttcagg ctgcatgcca tcaacaggat
    10321 aacttggcat atcataggca agacctattt tatacatatc caattggttg gtagtgcgct
    10381 cgagcgacgt tcccatggca taaccgttgt tttcgcaaat gaaaatcaca ggtatcttcc
    10441 acaacatggc catattgaat gtctcgtgaa cagatccctg acgcacggcg ccatcgccaa
    10501 agtaacagag acaaacattg cccgttcctt tgaattgttc tgcaaaacct atccctgctc
    10561 caagcggtat ctgcgcacca acaataccat gtccgccaaa gaaatagttc tccttgctga
    10621 aaaagtgcat agaaccaccc ttaccttggg taacccctgt cacctttccg aaaagttccg
    10681 ccatcgccgc attacaagaa atgccttttg caattgctag tccgtggtcg cggtaagcag
    10741 tgataatagg gtcttcatgt cgtatcgcac taatcgtacc ggcagctaca gcttcctgtc
    10801 cgatgtagag atgacaaaac ccgcgaatct tttgttgacc gtataattgc cccgctttct
    10861 cttcaaagcg acgcatttta tacatcagtt cgtaccagtt gaggtaggtt tctttggtta
    10921 tctttgagcg atccattatt cgtgccgaaa aatcgaaggg caaagttacg aaaaggagac
    10981 cacaatcaaa acaaccgtgt tactgcgcag tatacgcctc ctgcacttta aaaactatgc
    11041 ttctgccgag gccgtttttt cgcatgcttt cacccttatc aacggtttga acggcagcgg
    11101 taaaacaaac ctgatcgacg ccgtctacta cctctgtctt tgtcgtagct acttcacccg
    11161 ttccgatagc agtgtgttgc aacatgacgc cccgtatttc aggattgacg gcactttcag
    11221 caatccggaa ggcagcctcc tcattacctg caaatacctg acggcgaaaa aagaagtgct
    11281 gcgaaatgag gtgccatacg accgcatttc cgaccatatc ggcaatttcc cggtggccat
    11341 gatagcgccg gacgacatca acatcatcaa cgaagggagc gaggagcgac gcaggtttct
    11401 cgacgccgct atatcgcaac tcgatcgcga atacctcaac aatctcatcc tttacaacaa
    11461 gtatctcgct cagcgaaacg ctttgtttaa gcaagctttg gggggaggaa tcaggcctga
    11521 tgctacattg gtgggagtac tcaaccacag tctcgcctta catggcaatg caatctaccg
    11581 cgcaagacgg gcttatctcg aagcatttac acccattttc cgcgatatgt attcccgcat
    11641 ttccggcggt acggaggatg ctgatatcag ctataagtca caactcgaag aacatgatca
    11701 cctgcacctg ctgttgcaca gtgaacgtga cgacagggct gcaggtcgca ctacatccgg
    11761 tattcataag gacgatctct atctgagtgt caagcaatat ccggtgcgcg ataccggaag
    11821 ccagggtcaa ataaaatcct ttcttatcgc cctgaaactg gctcaatgtt atatgatgtt
    11881 tcgcagtgaa ggcaaacaaa gtatactctt gctcgacgat atcttcgaaa aactcgacaa
    11941 gcagcgattg gaagtattgt tcaacatgtt gcaggatgaa gtgttttgtc aggtattcat
    12001 caccgatgca gataccaaac gaagcatgga attttgtaaa tcgcacctgc cggatttcgg
    12061 acatatacat gtaaacaacg gtaaaatcga gtacctttaa tccaagatga aaaaggacaa
    12121 tcaactcagt atcggtgatg ccattaaaca gctgtttcac agcatccgca ttgaggataa
    12181 agtggctgaa gtacgcatta aggagttgtg gccttccata atgggtgcat ccattaatcg
    12241 atacaccggc gagatttatt tcaacaaagg catactcacc atttacatca actccgcacc
    12301 tttgaaacaa gacctccaat tttctaaaca ggagatcatc gaaagggtga atgaagaact
    12361 ccaagaaaat ctagttcgag aagtaataat tagatgaagg aaaaaggaaa aaggagagag
    12421 gagaaaggag agaggagaaa ggaaattctg gtgtggaatt atttgttcga ggtcggtggg
    12481 ggatttggtt gttcaacctc ttcggctctg cgggaactaa gtggcgtgaa gatgttactt
    12541 atgatgccaa cggtaacatt ctgacttatt tccgtaatgg caacacctcg acaatggaca
    12601 acctgagtta cgtatataac acgggtaata accagttaaa gcgtgtagat gatgccgtct
    12661 catccggtac gtatagcgta gacattgatg accaaagcgg cactaattac gaatatgatg
    12721 ctttgggtaa ccttgttaaa gatgtggcag aggaaatcga taccattttg tggacattaa
    12781 caggcaaagt ggatagtatt tcgcgaacag gtgcaagtac taaggatgaa ttaagcttcc
    12841 tgtatgaccc tatgggaaac cgggtaggta agattgtgta tgaagacgat ggcgacatac
    12901 tgaaaacatg gtatattcgc gatgcacagg gtaatatcat gtcaacttat acagaaaagg
    12961 aagactcatt gtggtggacg gaagtagatc tctatggttc aagtcgactc ggtgtcattt
    13021 atccggacag cctggtatac ccttatacat acacaatgcc gggcgatact ttctcgttct
    13081 tcaatattgc aggcaacaga agatatgaaa tcaccaacca cttaggaaac gtgttagcta
    13141 caattacaga taggcgaatc ccacaagaag ttggcacacc taacggcatt gcagaatact
    13201 atttggcaga tattgctagc gttcaggatt actatccgtt cgggatggta acgccgggac
    13261 gctcctattc cgctggaagt g
//
LOCUS       DAOQOU010000089        13221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2414_length_13221_cov_7.409540, whole genome shotgun sequence.
ACCESSION   DAOQOU010000089 DAOQOU010000000
VERSION     DAOQOU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_13221_cov_7.409540"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1415
                     /gene="nadA"
                     /locus_tag="PLM90_08815"
     CDS             402..1415
                     /gene="nadA"
                     /locus_tag="PLM90_08815"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMZ89514.1"
                     /translation="MIIGGEKVVDIEKLERTGFVDVDVDPSLDLFAEINKLKKEKNAV
                     ILAHYYQEPDIQDIADYIGDSLGLAQHAEKTNADMIVFAGVHFMAETAKILNPDKKVI
                     MPDAKAGCSLADSCPPALFRKFKEQHPGHTVISYINCTAEIKALSDIICTSSNAVKIV
                     ESLPADEPIIFAPDKNLGRYIEKKTGRKMVLWEGSCMVHEIFSLEKITKLKHRHPKAK
                     VIAHPECEEPVLAIADFIGSTTGLLKYATTDSAQEYIVVTETGIIHQMQKSAPQKTFI
                     PAPPTNLCACNDCPHMKRNTLEKLYISMKYELPEINIAEPTISLARNSIRRMLDISAK
                     AGL"
     gene            complement(1425..1748)
                     /locus_tag="PLM90_08820"
     CDS             complement(1425..1748)
                     /locus_tag="PLM90_08820"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ89515.1"
                     /translation="MAKQNIIVFNDVGSGELHSAQVTLRALTHPLRLKIISFIDKHRK
                     INVHKIYTGLNLDQSIASQQLNILRGANLVITHREGKFIYYSLNYDTIDQMNKLVEDF
                     RKNGK"
     gene            complement(1750..2085)
                     /locus_tag="PLM90_08825"
     CDS             complement(1750..2085)
                     /locus_tag="PLM90_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ89516.1"
                     /translation="MPAIHTIKLNTDNGAEIRVDYNHLRKTVLVIRSVNHKLRQQMIK
                     LLEEYPQLTVTEIYIKMRLEQSIASQHLAILRRSGVVITKRSGKFIYYSLNQDRLNDI
                     GRLIRELAK"
     gene            complement(2177..2854)
                     /gene="tsaB"
                     /locus_tag="PLM90_08830"
     CDS             complement(2177..2854)
                     /gene="tsaB"
                     /locus_tag="PLM90_08830"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMZ89517.1"
                     /translation="MARILHIETATPVCSVSIAASGITQAVLTTSSGNDHMSVITGNI
                     LAVCEQAGIGLKDIDAVAVSSGPGSYTGLRIGASTAKGICHALNKPLIAVPTLLAMVS
                     GIQEKYQEEALLFCPLMDARRMEVYTLVADRDCNISAPARAYVIEEPYFDFIPAGRKT
                     VFFGSGVEKTLNFLPEGSISDLHYFPSAEHLHLLALQQFNRQIFADTAYFEPEYGKEF
                     YTPAKKQ"
     gene            complement(2855..4522)
                     /locus_tag="PLM90_08835"
     CDS             complement(2855..4522)
                     /locus_tag="PLM90_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMZ89518.1"
                     /translation="MKICLPAASRRLLIPLLFISAQAVAQQEVITRFEKTISYLADDK
                     LEGRATGSAGEQLASAYIIQQFNEIGLTPAGDAGTYLQSFEARSGKKPGEENYIIAAS
                     RYTMDKLHYPHPMTGNGIASGNLIAVGFGITAPALSYDDYAGKDVKGKIVLIKTSSPD
                     GTHPHSKYIEFNDERKKIQLAAANGAAGVIFYNEDANYELPGDDYRRNVTAENIPVFI
                     VDSTTAKSLLQFTGTVELHTQLNEEVRTGHNVLGFIDNHASNTVVIGAHYDHLGHGEI
                     EGSLYRGAPAIHNGADDNASGVAMMIELARFLKQMPSSNNYLFIAFSGEEMGLFGSKA
                     FTQSPMMLQYQFNYMLNYDMVGRMDTTNAIIINGVGTSPAWATLKSISNFDMTLTTTE
                     SGIGPSDQTSFYLKDIPVVHFFTGSHADYHKPSDDAGLINYMGMLKVFKYSIALISAL
                     DDDGKLAFTKTQDSNNENTPRFKVTMGVVPDYAYSGKGMRVDGVTDGKPAQAAGVIAG
                     DIVIKLGSFDVTDMMGYMTALSKFTKGETVTLVVLRNNAPVELQVTF"
     assembly_gap    4653..4752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4753..5801)
                     /locus_tag="PLM90_08840"
     CDS             complement(<4753..5801)
                     /locus_tag="PLM90_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ89519.1"
                     /translation="MQSRNKMKLTNRFIAGLIGVATIFIGTSAATSADLYEVGKNIDI
                     FTSIYKELNTYYVDDVDPNKLMRTGIDAMLESLDPYTNYISEAEIEGYKFQITGNYGG
                     IGASIRTIEGKSTVMECYPGFAADKAGIIPGDIITEIDGKKLNGDNNESVSDLLRGSP
                     GSTVNIKIYRPYADKNIDLTVTREDIDVHNVPYSGFVKDHIGYIILEQFTEDAGLHVG
                     NALKDLKAKDPALSAVILDLRSNPGGLLREAVNVSNVFINKGEEVCSTLGKVKEWDKT
                     FNTLNNAVDANIKLVVLTNSSSASASEIVAGTIQDYDRGVILGQKSFGKGLVQTTRDI
                     SFNTKLKLTTAKYYIP"
     gene            complement(5813..6031)
                     /gene="yidD"
                     /locus_tag="PLM90_08845"
     CDS             complement(5813..6031)
                     /gene="yidD"
                     /locus_tag="PLM90_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HMZ89520.1"
                     /translation="MNIFSYIIIIPVKLYQWVISPILPNSCRYTPTCSHYMLDAVKKY
                     GPIKGGWMGLKRLSRCHPWGGSGYDPVK"
     gene            complement(6031..6426)
                     /gene="rnpA"
                     /locus_tag="PLM90_08850"
     CDS             complement(6031..6426)
                     /gene="rnpA"
                     /locus_tag="PLM90_08850"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMZ89521.1"
                     /translation="MLRKSERLCQQQLIASLFTAGHRFLSRPFQLVVLTTPLNSPEPA
                     QLLISVSRKRFPRAVDRNRIKRYLREAYRHHKGELYTYLQQHNMQVAFAVVYTGKSVT
                     TFNEMNDRIPVALSLIVKQLETLRSKSEG"
     gene            complement(6461..6619)
                     /gene="rpmH"
                     /locus_tag="PLM90_08855"
     CDS             complement(6461..6619)
                     /gene="rpmH"
                     /locus_tag="PLM90_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HMZ89522.1"
                     /translation="MKRTFQPSKRKRKNKHGFRERMKTKDGRKVLARRRRKGRKKLTV
                     SDERNISK"
     assembly_gap    7125..7224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7225..8648)
                     /locus_tag="PLM90_08860"
     CDS             complement(<7225..8648)
                     /locus_tag="PLM90_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HMZ89523.1"
                     /translation="MKHFAASILVILLGTFVAKAQITAFPDTSICPGDLVTLGSVTVD
                     ICADCYSYEEIPFAPETIGGESITMVDDTYIGPYDIGFDFCFFGVYYDQFYLCSNGWI
                     SFVPPGGGWATNWTPDGPIPDGASNVPKAAIFAPWTDWHTGLCTDCIHYEVFGVTPNR
                     RIAITWEEVPLFSCTDYLGTFQIVLYETSNWIDNHFMDVDICPTWDLGIATQGLQNED
                     ATAAYTVTDRNATAWSATDESWRWYTSILEWYDEDGVLVGTGPEVDVSPEFTTTYTLV
                     QTLCDGTSYTDDVTVTVGADLTIDSDITAVSCGGAGDGSISISVDGGIEPYTYEWSTG
                     ATGVTELSGLDGGTYTVTVTETGGCSRTYSFDVIEPEELVAEATDIVNNPCNGYDEGS
                     VHIDVTGGSIPYSYSLDGGASTILSDFTGLEAGDYTVTVTDASGCTTTAEFTITEPIL
                     LTADLTADDTEIFVGQGSTITLTT"
     gene            8821..9015
                     /locus_tag="PLM90_08865"
     CDS             8821..9015
                     /locus_tag="PLM90_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89524.1"
                     /translation="MTMLEYAKMILQKVSFDQGLFRTELYKAIRKLMQEEVSELKKWC
                     LETFGVSYCMEAAPQLLIEG"
     gene            complement(9209..10882)
                     /locus_tag="PLM90_08870"
     CDS             complement(9209..10882)
                     /locus_tag="PLM90_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HMZ89525.1"
                     /translation="MIRAANFKASDTISFRYIYIYVLLMYGCANVVTPTGGEKDVLPP
                     VALSFSPDTNSLFFNTNEIKITFDEFIQLSDAFNQVLISPPLNNTPDYKVKGKTLTIK
                     LNDTLKENTTYTINFGQSIKDITESNILNNFTYVFSTGEQLDSLQVKGRSVDMLSGKP
                     SEKAYAVLYYEPTDTSFTTSRPYYFARIDKEGNFTIKNIRSGKYRLYVLEDMNFNYYY
                     DLPNERIAFQDSILEVNGEIPLQKMSVFSEDKLPQSLTDIKSPRYGMTRMVFAKNASN
                     VSIKYTGTDTAETYIARNVTGDTLTFWHSNYALDSHKLAIQFDTTLLDRTIAIKTFPK
                     DSDFTKQHNTFTTNAIPINKGAGARADWDPERDIILKCYNPVTQLSTSPKVYSDSILL
                     GDATWFTDTLDATKVYIRYPFKPETNYDIRIEAGNMHDIFGLTNLADTIFIKTRKLDA
                     YGTLNIALTNSSNSQLIVELIKFDLSPVQTWVLPVEQFQNETHTFNIKKSYLLPGVYR
                     IRVTKDTNGDRKWTTGELVQYRQPETIIYYPTDQNVRANWDNEIEWEIK"
     gene            complement(10879..11244)
                     /locus_tag="PLM90_08875"
     CDS             complement(10879..11244)
                     /locus_tag="PLM90_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89526.1"
                     /translation="MQIEKLIPVLWTEHVEESIHFYTEILPFQLSAHDREANWANVVW
                     GEIEIMFSTPNAHVEQRAIGFTGSFYFRCTDVETLYHAISTKVKLIYPLEVFSYGMKE
                     FGCYDNNGYILNFGEQIDQ"
     gene            complement(11252..13114)
                     /gene="mnmG"
                     /locus_tag="PLM90_08880"
     CDS             complement(11252..13114)
                     /gene="mnmG"
                     /locus_tag="PLM90_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMZ89527.1"
                     /translation="MFKEYDVIVVGAGHAGCEAAAAAANMGSSVLLVTMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVTDKTAIQFRMLNRSKGPAMWSPRAQSDRMLF
                     ANEWRLMLERTPNVDFWQDMVSGLLVKDGRVCGVRTGMGIEIPSKAVVLTNGTFLNGL
                     IHIGEKQFGGGRAAEKAATGITEQLVELGFESGRMKTGTPPRVDGRSLDYSLMEEQPG
                     DEDPQKFSYTDTKPLAKQRPCHITYTSDAVHDILRTGFEKSPMFSGRIKGPGPRYCPS
                     IEDKIERFSSRERHQIFVEPEGWDTVEVYINGFSTSLPEDVQYKALKAIKGFEHVKMF
                     RPGYAIEYDYFPPVQLNHTLETRLVKNLFFAGQINGTTGYEEAACQGLIAGINAHLAV
                     KEDAPFVVDRSEGYIGVLVDDLINKGTEEPYRMFTSRAEYRVLLRQDNADIRLTPRGH
                     AIGLASDARLERVRQKEQASADLIKFLQQTSIDPDQINGRLEMLGSAAIRQKTKLALL
                     LSRPHIEMQHLLGLNEALDATILSFEPEFREQAEIIIKYEGYISKEKELVEKMQRLEH
                     IVIHDTIDYSLIHSLSSEAREKMQRIRPKTLGQASRISGVSPSDIAVLMVHIGR"
BASE COUNT         3591 a         2936 c         2573 g         3921 t
ORIGIN      
        1 cgcgttgcga gcgaatttgc atagtgccca ctcaccactc accactcacc cactccctgt
       61 ggattagcag ttaactgact gttagtacct atggataacc ccatgagtta tcccagcgtc
      121 aaatttccac agccaaataa tgtgcagtac cctaggtagt accaacatgg tacgtacgtt
      181 gacggattgt gaacagacaa aaagttttcc tgttaatttt gtaccaacat tgtgctctat
      241 ttggtggata tccccataaa tcgggaacct caactgtgta tatactgttt gtacatccaa
      301 taaaactatg gaaaacgctt tttccaaaaa tgtgtatcaa ttcttatcca ccaatacaca
      361 gcactgatga tgattcttta tatttatata aaatataatt catgataata ggaggagaga
      421 aagttgtgga tattgaaaaa ctggagagaa ccggttttgt cgatgtggat gtagatccat
      481 ctcttgatct gtttgccgag atcaataaat taaaaaagga aaaaaatgcc gtcatcctgg
      541 cgcattacta ccaagagcct gatattcagg atattgcaga ttatataggc gatagcctcg
      601 gtcttgcaca acatgccgaa aagacaaatg cggacatgat tgtgtttgcc ggtgtgcatt
      661 tcatggcaga aacagctaaa attctcaatc ccgacaaaaa agtcatcatg cctgatgcta
      721 aagcaggatg ttcacttgcc gatagttgtc cgcccgccct tttcagaaaa ttcaaagagc
      781 agcatcccgg acataccgtt atcagttata tcaattgcac agccgaaata aaagcgctca
      841 gcgatattat ctgcacaagc agtaatgccg ttaagattgt tgaaagcctg ccggcggatg
      901 aacccattat tttcgcaccg gacaagaacc ttggcagata tatcgagaaa aaaacaggac
      961 gcaaaatggt gctgtgggaa ggatcttgta tggtacatga aatatttagt ctcgaaaaga
     1021 ttaccaaact caaacaccgt catcctaagg caaaggtgat cgcccatccc gaatgtgaag
     1081 aaccagtatt ggccatcgcc gattttattg gcagtacaac aggtttactt aaatacgcca
     1141 ctaccgattc tgcccaagaa tatatcgtag tcactgaaac cggtattatc caccaaatgc
     1201 aaaagagcgc ccctcagaag acttttattc ctgctccgcc aaccaaccta tgcgcatgca
     1261 acgattgtcc tcacatgaag cgcaatacgc tcgagaagct atatatcagt atgaagtatg
     1321 agctgccgga aatcaatatt gctgagccaa ctatttcttt ggcaaggaat tcaataagac
     1381 gtatgctgga tatttctgca aaagcaggat tgtaaaagaa aatcttattt accgtttttt
     1441 ctgaaatctt cgacaagctt attcatctgg tcgatggtat cgtaattgag cgaatagtaa
     1501 atgaatttac cctctctatg tgttattacc agatttgctc cgcgaaggat atttaactgt
     1561 tgactggcaa tactctgatc caaatttaat cccgtataaa tcttatgtac gttgattttg
     1621 cggtgtttat cgataaacga aatgatcttc agtctcagcg gatgtgtcaa agcacgcaaa
     1681 gttacctggg ccgaatgaag ttcaccgctg cccacatcat taaatacgat gatgttttgc
     1741 tttgccatac tattttgcca gttcccgtat cagtcgccca atgtcattca aacgatcctg
     1801 attcaaagaa taatagatga acttaccact gcgcttggta attacaacgc cactgcggcg
     1861 caatatcgcc aaatgctgcg aagctatcga ttgttcaagt cgcatcttga tatagatctc
     1921 cgttaccgtt aattgcggat actcctccaa cagtttaatc atctgttgac gcaatttgtg
     1981 gtttaccgac cttatcacta acaccgtctt tcgcaaatgg ttgtaatcca cgcgaatttc
     2041 ggcaccgttg tcggtgttga gctttattgt gtgtatagcg ggcatagggt gtatgtttgg
     2101 gtcacgatac ccactctgac cttcagttag taaagctatc aataatcatt ctaaaaaaca
     2161 aatcggcaac agcttatcac tgtttttttg caggagtgta aaattccttc ccatattccg
     2221 gttcgaaata tgcagtatca gcaaaaatct gcctgttaaa ttgttgaagt gctaacaaat
     2281 gcaaatgttc ggcagaggga aaataatgta aatctgatat ggatccttcc ggaagaaagt
     2341 ttaaagtttt ttctacacct gaaccgaaga aaacagtttt ccttcccgca ggaataaaat
     2401 caaaatatgg ctcctctata acataggctc gtgcaggagc cgatatgtta caatctctgt
     2461 cggcaaccaa ggtatacact tccatacgcc tggcatccat caagggacaa aatagcagcg
     2521 cttcttcctg atatttttcc tgtataccgg aaaccatggc caacaaggta ggcacggcaa
     2581 taagtggttt gtttaaagcg tgacaaatac cctttgccgt tgatgcacca atgcgcaaac
     2641 cggtataaga acccggcccg gagctcacag ccactgcgtc tatatccttt aaccctattc
     2701 cagcctgttc acaaaccgct aaaatattgc ccgtgattac agacatatga tcattgccgg
     2761 aactagtggt caatacagct tgtgtaatac ccgacgctgc aatactcaca ctgcaaaccg
     2821 gtgtggctgt ttcgatatgt agaatgcgtg ccaatcagaa tgttacctgc aattctacag
     2881 gtgcattatt gcgtaataca actaaagtaa ctgtttcacc tttcgtaaat tttgacagcg
     2941 ctgtcatata acccatcata tctgttacat caaagcttcc cagtttgatc acaatatcgc
     3001 cggcaatcac gcccgctgct tgtgcaggtt ttccatcggt aacaccatcc acgcgcatgc
     3061 cctttccact ataggcataa tcaggaacca cacccatggt tactttaaat cgcggtgtgt
     3121 tttcattatt gctgtcttgt gttttggtga aggcaagttt gccatcatca tcaagtgcag
     3181 aaattaatgc aatgctgtat ttaaatactt tcagcatgcc catgtaatta attaaaccgg
     3241 catcatcact tggcttgtga taatcggcat gcgatcctgt gaagaaatgc actacaggaa
     3301 tatcttttaa ataaaatgac gtctgatcac tcggcccaat tccactctct gttgtggtga
     3361 gtgtcatgtc gaaattggat atgcttttta atgttgccca cgccggacta gtaccaacac
     3421 catttatgat gatggcattt gttgtatcca tacgaccaac catatcataa ttcaacatgt
     3481 aattaaattg gtattgcaac atcatcggtg attgcgtgaa cgccttgctc ccaaacaatc
     3541 ccatctcctc tccgctgaat gcaataaata aataattgtt tgacgatggc atttgtttca
     3601 ggaacctcgc taattcaatc atcatcgcaa caccgctggc attatcgtca gcaccattgt
     3661 gaatggcagg tgctccgcga taaagcgatc cctctatttc accatgtccc aaatgatcat
     3721 agtgagcacc tattaccacc gtatttgatg cgtgattatc tataaaccct aacacgttgt
     3781 gaccggtgcg cacttcttca ttcaattgtg tgtgtaattc aaccgtaccg gtaaattgta
     3841 ataaactttt tgcagtggtg ctgtccacaa taaataccgg aatattttct gctgttacat
     3901 ttctgcgata atcatctccg ggtaattcat aatttgcatc ttcattgtaa aatattacgc
     3961 ctgctgcacc atttgctgct gctaactgaa ttttttttcg ctcatcatta aattcaatat
     4021 acttggaatg cggatgtgtg ccatcgggcg aagaagtttt tattaatacg atttttcctt
     4081 ttacatcctt accggcataa tcgtcgtatg ataaagccgg agcggtgata ccaaatccaa
     4141 ctgctattaa atttccggaa gctattccat ttcccgtcat cggatgcgga taatgtagct
     4201 tgtccatggt atatcgcgat gcggcgatga tgtaattctc ctcacccggt tttttgcctg
     4261 atcgcgcttc aaaactttgt agataggttc ctgcatctcc tgcaggtgtc agacctattt
     4321 cattgaactg ttgaatgatg taggccgatg ccaattgctc tccggcactt cctgttgcac
     4381 ggccttcgag tttatcgtca gcaaggtagc tgatggtttt ttcaaatcgt gttattacct
     4441 cttgctgagc aacagcttgt gcgctgatga agagaagagg tatgagcaga cgacgacttg
     4501 ccgccggtag acaaattttc ataactgcaa aattaatgcg ccccgagctt ttccggcgat
     4561 ttaattgtca ccgggatcgg ttatttcggt tgaagaagcg aattgtatgt tgcctgatct
     4621 tccaaaacct ctattgcccg ctgaacatca tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnggaatata atatttagcg gtggtaagtt tcaatttggt gttgaaggaa
     4801 atatctcttg tggtttgcac aagtccttta ccaaaacttt tttgtccgag aataacacct
     4861 ctgtcataat cttgaatggt ccctgcaaca atttctgatg cagaagccga tgaagagttg
     4921 gtaagcacca ctaatttaat attcgcatca acagcattat tcagtgtatt aaatgtttta
     4981 tcccattctt taactttacc taatgtgctg caaacctctt ctcccttatt gataaataca
     5041 tttgaaacat ttactgcttc gcgcaaaagt ccgcccgggt tgctgcgcag atcaagaatc
     5101 acggctgata atgcaggatc ctttgctttt aaatctttca atgcattgcc tacgtgtaat
     5161 cccgcatctt cagtgaactg ttccagaatg atataaccga tatgatcttt cacaaatcct
     5221 gaataaggta cattgtgtac atcaatatct tcgcgtgtta cagtaaggtc tatatttttg
     5281 tctgcatatg gccgatatat tttaatatta accgttgacc ccggtgatcc gcgcaacaga
     5341 tcacttaccg attcgttgtt atcgccattt aatttcttac catcaatttc agtaataata
     5401 tcacccggaa taattcccgc tttatcagct gcgaatccgg gataacattc cattactgtc
     5461 gacttgcctt ctatagttct gatactcgca ccaatgccgc cataatttcc tgttatttga
     5521 aatttatatc cttcaatctc agcttcagaa atataattcg tatatggatc caaactctcc
     5581 aacatcgcat caattcccgt gcgcatcaat ttattcggat caacatcatc aacataatag
     5641 gtatttaatt ctttgtagat agaagtaaat atgtcgatgt tcttgcctac ctcataaaga
     5701 tctgcgctcg ttgctgcact ggtgccgatg aaaattgtcg ccacacctat cagtcctgca
     5761 ataaatctat ttgttaattt cattttgttt ctgctctgca ttaattcata aattatttca
     5821 ccggatcata gccacttcca ccccagggat ggcaacgcga taatcttttt aatcccatcc
     5881 agccaccttt aattggtccg tattttttta cggcatcaag catgtagtga ctgcatgttg
     5941 gggtataccg acaactattg ggaagtattg gtgatatcac ccattgatac aattttacgg
     6001 gaataattat gatgtaagaa aaaatattca tcacccttct gatttcgatc gaagtgtttc
     6061 taattgtttt actatcaacg ataaagctac gggtatcctg tcattcatct cgttaaaagt
     6121 tgttacagac tttcctgtat aaacgacggc aaatgctact tgcatattgt gctgttgcaa
     6181 ataggtatat aattcgcctt tatgatgacg atatgcttct cgtaaataac gtttaattcg
     6241 gttgcgatca acggcgcggg gaaagcgttt gcgcgatacg gaaatgagta actgtgcggg
     6301 ttccggggaa tttaatggtg tggttaacac caccagttga aagggtcggc ttaaaaaacg
     6361 atgccccgcc gtaaatagcg aggcaataag ttgttgctga cacagccgtt cagacttgcg
     6421 cagcatggca tccggcaact tagcgctacc ggaaccaata ttatttgctg atattccttt
     6481 cgtcagaaac ggtcagtttt ttgcgtcctt tacgacggcg acgtgccaat actttacggc
     6541 cgtctttggt tttcatgcgt tcgcgaaaac cgtgtttatt ctttcttttc ctttttgagg
     6601 gctgaaacgt ccttttcatc actaattaat ttgggaacgc aaagataaca ttacctcacc
     6661 gaaattcaca atctgtccac aaaattaatt gccaacaatt tcgaacactg aaaaccgtgg
     6721 tttctgttta gggtttagct tcttttaagc aggtaaatga attctgatat ttcatttcgc
     6781 ctaaaactat tactatactt ggtctatgcg ataatgcata taataaaaac ataatatgta
     6841 atatattaaa atttgtgttg atatgttacc cgcaaagcat taaacagtaa tggttttgga
     6901 gtaaaacagt tgcaccccga aaaagagtgg ttttgggtta aatcgaatag tggttgtcgc
     6961 aataattatc ttactagtac gaagtttcct gtttcagaga acttttcacc attgataaag
     7021 ccggcatcaa cctgccaaac ataagtgccc atgccctgtg gctcaccatc ggtaacgcca
     7081 ttccagcccg gacccgctat atcattggtt tcgaacatga cttcnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnctggtg gtcagtgtaa tggtagagcc ttgtccgaca
     7261 aatatttcgg tatcatctgc agttagatct gcagttaata atattggttc tgtgatagtg
     7321 aattcggcag tagttgtgca accgcttgca tcggttaccg ttactgtata atctcctgct
     7381 tccaacccgg tgaaatcact caatatggtg gaggcgccgc cgtccagact ataggaataa
     7441 ggtatactac ctccggtaac atctatatgt actgaccctt catcataacc attgcaaggg
     7501 ttgttgacta tatcggtagc ttcggcaacc aattcttcgg gttcaattac atcaaaagaa
     7561 taagtgcgcg agcatccacc tgtttctgta acagtaacag tatacgtgcc gccatccagg
     7621 cccgacaatt cggtaacgcc tgttgcgccg gtgctccatt cataggtata aggttcaatt
     7681 ccgccgtcaa cagaaatact gatggagcca tctcctgcgc caccgcagct tacggcagtg
     7741 atatcactat ctatggtcaa atcggcacca acggtaacgg ttacatcatc ggtataagaa
     7801 gtaccatcgc aaagcgtttg caccaaggtg tatgttgttg tgaattcggg tgaaacatca
     7861 acttccggac ctgttcctac taatacgcca tcttcatcat accattccaa aatactggta
     7921 taccagcgcc aggattcatc agttgccgac caggctgtag cattgcggtc agttacggta
     7981 taagctgctg ttgcatcttc attttgtaaa ccctgtgttg caatacccaa atcccaagtc
     8041 ggacaaatgt ctacatccat gaaatggtta tctatccaat ttgaagtttc atataataca
     8101 atctggaatg tgccgaggta atctgtacag ctgaacagcg gcacttcttc ccaggtaata
     8161 gcaatacgac ggtttggggt aacgccaaaa acttcatagt gaatacaatc ggtacacaag
     8221 cccgtatgcc agtcggtcca cggcgcaaag attgccgcct tcggaacgtt ggaagctcca
     8281 tctggaatcg ggccatcagg cgtccagttg gtagcccagc caccgccggg tggaacaaag
     8341 cttatccatc cgttagagca gaggtagaat tgatcatagt acacaccgaa gaagcaaaaa
     8401 tcgaaaccga tatcatatgg tccgatatag gtgtcatcta ccatcgtgat cgactcaccg
     8461 ccgattgttt ccggggcgaa aggaatttct tcataactat agcaatcggc acaaatatcc
     8521 accgttaccg atcctagcgt aaccaggtcg cccggacaaa tactggtgtc gggaaatgct
     8581 gtgatttgtg ctttagcaac gaaagtgccc aggaggataa ccagaatgga ggctgcaaaa
     8641 tgcttcataa cacgatgaat atgaacgata aagataatga tttgccggaa agccaaaaac
     8701 gcagtgtctt atttagtagg atttcgttcg aaatctgtga aaaattgcca attgttaaag
     8761 ctgtaaaaaa tgcaacattt gcatccaata cctcgtttta ttaactcaaa ggccaggaca
     8821 atgacgatgc ttgaatacgc caaaatgatc cttcagaagg tgagttttga tcaaggactt
     8881 tttcgtaccg aattatataa agccatcaga aaattgatgc aggaagaagt cagcgaactg
     8941 aaaaaatggt gtctcgaaac atttggcgtt tcctactgca tggaagccgc ccctcaactc
     9001 ttgattgagg gttgatttga ttatttgccg tttattttcg atttaagcaa cgcagaatgt
     9061 ttttgatatt ctgccatcta aatttctttt tcttttaact gcgcgcagct gtgagatcga
     9121 ctttttaagg ctgtttgaaa tttttgaagt gagcatttga tatgtgcgaa ggagaaaaat
     9181 caatttcaaa tcgtacgaat aattgccatc attttatctc ccactcaatt tcattatccc
     9241 aattcgcgcg aacattttga tcggtgggat aatatattat tgtttccggt tgtcgatatt
     9301 gtactaattc tcccgtcgtc cattttctat cgccattggt atccttcgtc acgcgtatgc
     9361 gatagacacc cggaagtaaa tacgatttct ttatattgaa agtgtgtgtt tcgttttgaa
     9421 attgttccac gggtaacacc caggtttgta ccggcgataa atcaaatttg atcaactcga
     9481 ctatcaattg cgaattgctc gaattagtca gtgcaatatt taatgtacca tatgcatcga
     9541 gctttcttgt tttaatgaaa atggtatctg caagatttgt cagcccgaaa atgtcatgca
     9601 tgtttcccgc ctctatccgg atatcgtaat ttgtttcagg tttgaacgga tagcggatat
     9661 atacttttgt agcatctaat gtatcagtaa accatgtggc atcgcctaac aaaatactat
     9721 cgctatatac ttttggtgaa gtagatagtt gcgttacggg gttatagcac ttcaaaatga
     9781 tatcgcgttc cggatcccaa tccgcccttg cacccgctcc tttatttata ggaattgcat
     9841 ttgtggtgaa tgtattgtgc tgtttggtga aatcagaatc ttttggaaat gttttgattg
     9901 caatggttct atctaaaagt gttgtgtcaa attgtatcgc aagtttatgt gaatcaagcg
     9961 cgtaattgct atgccagaag gttagcgtgt cacccgtaac attgcgtgcg atataggttt
    10021 cagcggtatc tgttcctgta tatttaatcg atacattcga ggcatttttt gcaaatacca
    10081 tacgcgtcat gccgtagcgg ggcgatttaa tgtcggttaa actctggggc aatttgtctt
    10141 cagaaaacac cgacattttc tgtagaggaa tttcgccatt cacctctaaa atggaatcct
    10201 gaaatgctat tctttcattc ggcagatcat aataataatt gaagttcata tcctccaaca
    10261 catataatct atactttcct gagcggatat ttttgatcgt gaaatttcct tctttgtcta
    10321 tgcgggcaaa ataataagga cgcgatgtgg taaatgatgt atctgttggt tcgtagtata
    10381 atacggcata agctttttcc gacggcttac cactaagcat atcaaccgac cttcctttta
    10441 cttgtaacga atccaattgt tcgccggttg aaaatacata tgtgaaatta tttaatatat
    10501 tagattcggt aatatctttt attgattgcc cgaagttgat ggtataagtg gtgttttctt
    10561 taagcgtatc atttaattta atggtgagtg tttttccttt taccttataa tcgggtgtat
    10621 tatttaatgg aggtgaaatc aatacctgat taaatgcatc acttaattga atgaattcat
    10681 caaaggttat ttttatttca ttggtgttga agaataatga attcgtatcg ggagaaaatg
    10741 aaagtgcaac aggaggtagt acatcttttt ctccgccggt gggtgtcacc acgtttgcac
    10801 atccgtacat cagcaataca tatatatata tgtatcgaaa tgaaattgta tcagatgctt
    10861 tgaaatttgc tgcgcggatc attgatctat ctgttcgcca aagttgagta tatatccgtt
    10921 attatcatag cacccgaatt ctttcatgcc gtatgaaaat acttccagag gataaatcaa
    10981 cttcacctta gttgaaatag catgatataa tgtttcaaca tctgtacatc ggaaataaaa
    11041 gctaccggta aaaccgatgg ctctttgttc gacatgcgca ttaggtgtgc tgaacataat
    11101 ttcgatttca ccccaaacca cattcgccca atttgcttcg cgatcgtgag ctgataattg
    11161 aaatggtaag atctctgtgt agaagtgaat gctctcttca acatgctcag tccacaacac
    11221 cggaattaat ttttctattt gcatgtgtga attatctgcc aatatgcacc atcagcacgg
    11281 cgatatcgga tggtgaaaca ccgcttatcc tgctggcctg accaagggtt ttgggtctga
    11341 tgcgttgcat tttttctctt gcctcagaag acagtgaatg aattaggctg taatcaatgg
    11401 tatcatgaat gacaatatgt tccagtcgct gcatcttttc taccagttct ttttccttgc
    11461 tgatatagcc ttcatactta ataatgatct ccgcctgctc gcgaaactcg ggttcaaagc
    11521 tgagaatggt agcatccagg gcctcattca accctaacaa atgctgcatt tctatatgcg
    11581 gtctgctgag caataacgcg agttttgttt tttgacgtat ggcggcactg cccaacattt
    11641 caagtctgcc gtttatttga tccggatcta tgcttgtctg ttgaagaaat tttatcaaat
    11701 cggcacttgc ttgttctttc tgtctcaccc tctctaaccg cgcatcagat gccaaaccta
    11761 tagcgtgtcc gcgaggcgta agcctgatat cggcattgtc ctgacgcagc aacacgcgat
    11821 attctgccct ggaggtgaac atgcgatacg gctcttctgt ccccttatta ataagatcat
    11881 ctaccagtac accgatatat ccttcactgc gatcaactac aaacggggca tcttctttta
    11941 ccgccagatg tgcattgatt cctgctataa gtccttgaca tgcagcctct tcgtaaccgg
    12001 ttgtgccatt gatctgccct gcaaaaaaga gatttttcac caacctggtt tccaatgtat
    12061 gattcaactg gacaggcggg aaatagtcat attctatagc ataccccggt ctgaacatct
    12121 tcacatgctc aaatcctttg atagctttta gcgccttata ctgcacatcc tcggggagac
    12181 tggtactaaa accattgata tatacctcca cggtatccca tccctccggt tcaacaaata
    12241 tctgatgacg ctctctggaa gagaaccttt caatcttatc ttcaatacta ggacagtacc
    12301 ttggtcccgg acccttgatc cttcccgaga acatcggcga tttctcaaac cctgttctca
    12361 gaatatcatg caccgcgtct gatgtatagg taatatgaca aggcctctgc ttggccaggg
    12421 gttttgtatc tgtatacgag aacttctgcg gatcctcatc gccgggctgt tcctccatga
    12481 ggctataatc caggctcctg ccatccaccc ttggcggtgt tccggtcttc attctgccgc
    12541 tttcaaatcc cagttccacc agctgctctg taattccggt agccgctttc tctgcagccc
    12601 tgccaccacc aaattgtttc tcgcctatgt gaatcagtcc gttcaggaag gttccattgg
    12661 tcaacaccac cgcttttgat ggtatttcaa tccccattcc cgttctcaca ccacataccc
    12721 ttccatcctt caccaacaat cccgacacca tatcctgcca gaaatcgaca ttcggagtcc
    12781 tttccagcat gagtcgccat tcgttcgcaa acaacatcct gtcgctctgc gctcgtggac
    12841 tccacatcgc gggtcccttc gacctgttga gcattctaaa ctgaatagcg gttttatcgg
    12901 tcacaattcc cgaataccca cccaaggcat caatctcccg gacaatttgc cccttcgcca
    12961 ccccacccat tgcagggtta cagctcatct gggcaatcgt ctgcatattc atggtcacca
    13021 acagcacact agaccccata ttagccgccg ccgctgccgc ctcacagccc gcatggccgg
    13081 cacccaccac tatgacatcg tattctttga acatgagagg gcaaagataa ggagaaagga
    13141 gagaggaaaa aggagagagg aaagaggagt gggtgagttt acctcgccgg gcaggcgggt
    13201 gggtgagtgt gatattgacc g
//
LOCUS       DAOQOU010000090        12720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2541_length_12720_cov_5.588788, whole genome shotgun sequence.
ACCESSION   DAOQOU010000090 DAOQOU010000000
VERSION     DAOQOU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12720
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_12720_cov_5.588788"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1527
                     /locus_tag="PLM90_08885"
     CDS             91..1527
                     /locus_tag="PLM90_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HMZ89528.1"
                     /translation="MLTNETFRNQAHQVVDWIADYLENIQQYPVKPSIAPGALRAALP
                     DEAPQSGEAMDDIWKDFLNMIVPGITHWQHPGFMALFPSNASYPGILGEIAVSGLGIN
                     AMMWDTSPSATELEQTVMEWLRKATGLPDGMLGVIHDTASMSTLTAIISARERYAQFQ
                     VNQHGFHGYDKLVMYCTDQTHYSIEKGAKAAGIGAAQVKKIATDDAHAMIATALTKQI
                     AEDKAAGLQPFMVVATLGTTSTLAFDPLEEIGNICVEHKLWLHVDAAYAGSAFFLNEY
                     RHFLQGIDHADSYVFNAHKWLFTNFDCSCYFVKDANHLINTFSANPDYLKRTEESVAM
                     FKDWGIPLGRRFRALKLWFVLRNYGISGIQDLLRKHIDIATNFRNQLLSDTQWELMAP
                     PVMNMFCVRYHPQGINDNEALNALNTALLKSINADGNYYLSGTIVKGMYMIRVVMGQT
                     HLEQVHAEGLLALMRSSANSINDFKALR"
     gene            complement(1528..2916)
                     /locus_tag="PLM90_08890"
     CDS             complement(1528..2916)
                     /locus_tag="PLM90_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ89529.1"
                     /translation="MDKRLLRRLFEKQNPIPFRDTTLHTYTKGYWTVQPFLIGIPYKY
                     YYSKYEVAGWEQVPTDKPVIFAISHRNAFMDSLAFVNCKGTQVWQLARGDAFNNPMMK
                     KLFIFFHMLPLWRERDGVDTKNMNQPTFEACADILYNNGMVGIYPEGNCINEEHIRPL
                     KKGICRIAFLAAEKYQFQPDIHIIPVGVMYSGAEKFKHTQLLNFGKPILLKEYYEQYR
                     SAPAQTINILKDDIEKAMKETATHVPHGTFHHDIVELSKMHARETLLNNNIPVTPLNS
                     FNEEKVISAKLDAYQHSDHEGMKRLVQNLHNYKRIRNQHNLKENTFDKSRSHTFSMVF
                     MALYFLLMFPVYIYGAIINYLPYKFPQQFVEKKIKQKIFWSSAKYVISLFLFPIYYLI
                     LFIITWTVLGSIVSGLIFLFSFPISGNIAYYYYFDFKKWLSALRFKRLPEHEKAALIQ
                     ARDSVYQNLASL"
     gene            complement(2944..3861)
                     /locus_tag="PLM90_08895"
     CDS             complement(2944..3861)
                     /locus_tag="PLM90_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HMZ89530.1"
                     /translation="MCDIIDAKQLNFDQNNLILLNCLLAFLMFGIALDLKVEHFKRLI
                     QYPRKAIAGILSQYVLLPFLTYLIVIILQPCPSIALGIFLLGACPGGNMSNFLSSLAK
                     GNVALSVTLTATSTLLAPIMTPLNFAFYGGLYPPTAELMQEISVEPMELFTTIVVILG
                     VPLIIGMLVNHFMPKFTALIRRPVQIVSVIIFLCFIVFAFLKNYDLFMEIIGMIFLLV
                     LLHNGLAMLGGYGLGALFKLDFADKKCLSIETGIHNAALGLIIIFSFFKGLGGMAVVA
                     GWWGIWDLVTGFIIATYWSRMAAKKTLQA"
     gene            3946..6342
                     /locus_tag="PLM90_08900"
     CDS             3946..6342
                     /locus_tag="PLM90_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMZ89531.1"
                     /translation="MRIFPADIPQVLEFEKVLHEVARYCDSSPGADRVARVRPLDKFD
                     EVKKMLLQTREMTHLLQYAQGFPHDKMFDIRPTLPLLAIENYVLSEQQCHEIHRVALL
                     MGSIIRFFNSNQEQYPVLQQFVSGTTYVSAISQSIKAVIDDAGHMRNDASPELVRILK
                     EKDDAMRSLGKVFQSALQKYRSAGFLSEVEESMRNGRRVLGIQSEYKRKIAGIIHDES
                     ETGRTVFMEPQEVVNIQNEIFELEREEKREIYRILKTLTADLAPHQQILDKYQQILGI
                     IDFTRAKGRYALETQSSMPQLEKQPIVCLYDARHPVLLSLHKKQHKQVVPLQAELDSN
                     NRILVISGPNAGGKSVAMKTIGLLQYMTQCGLLIPASSHSRIGIFKQIFCDIGDTQSL
                     EDELSTYSARLMKIRHFLQHADRETLLLIDEFGSGTDPVMGGAIAEAALRMLNEKKIF
                     GVITTHYANLKSFAANAIGLFNGSMLYDEAELKPMYLLETGKPGSSYAFELAKKSALP
                     GTLIEEAKKLISKEHIRFEELLKNVRIEKEHIRLRDKEVSKKEEQLKKKEVELAAAME
                     KTREQREKYNLKKLEKEDQTIREMEATFKNLLQEFKQVQSDPEMDNATKEQSKDTVRK
                     FFQEQRKKTFDHQKKIRPTPIPQWQRGEIKIGGYVKLNSGSEIGVLESVKNNKATVIF
                     NNIRTTVPYADLIGIAKTEAPAITPAKIKVTIDESDFNTELDLRGQPREEAMMNLEQY
                     LDAAMMRKIYQLRILHGKGTGAIREVVQQTLKSHPAVLRFEFANREGGGDGVTLVTLG
                     "
     gene            6390..7721
                     /locus_tag="PLM90_08905"
     CDS             6390..7721
                     /locus_tag="PLM90_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89532.1"
                     /translation="MRNLLLLGIIFIHASLSAQCFIGIDSIYCTNGIPDTILPVDTGG
                     YFDGDGISANGIFFPEILDPGSYVISYKNTQYYYVDTTGVFAPLSGDEQYVSLIDDGF
                     SDGIEIGFDFEFFGSLKNYVRIHSNGFITFNFDLAIFPPYANDIPTPGMPTDLIAASW
                     GDYDPTMGGTISWFQSGSAPNRKLVINYRDLPPYGETEGLLTTQVILFETTNIIEIHT
                     TASPDYGKRKTQGIENIDGTAGYVRPGRNNSYWETYNDYVAFIPQFCIDTLYVEDPPV
                     TEVLDDEINICMPYAGLLHVSGADTYLWLSDDICTMTPGPDPDSYYLHGIAEGTFTCN
                     IVGFNEAGCSDTSQVVVNIETCQTAITNNVETPFQITPNPANNILTIIIAANEFNGKW
                     SLVNLQGAEMQFGELTGLKTEIDIRELPAGIYFLIFDAGDCSAREQFIISH"
     gene            complement(7804..7971)
                     /locus_tag="PLM90_08910"
     CDS             complement(7804..7971)
                     /locus_tag="PLM90_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89533.1"
                     /translation="MRFPELPFGCPRPLLLTAPDSHQVFQIYLVTAFKPIEIGIFLRT
                     AFAQGLCTNGG"
     gene            complement(8193..9266)
                     /locus_tag="PLM90_08915"
     CDS             complement(8193..9266)
                     /locus_tag="PLM90_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ89534.1"
                     /translation="MIDKNIRIAATPPGDFYLGEDQYALVREKVFTRSWHYIGDKHQF
                     QETGYLYPVNLFPGCLNEPLILSVQDDVLQLMSNVCTHRGYVLLTEKTKSQVIRCKYH
                     GRCFHPDGAFMSMPEFEEAENFPTRMDDLAKAKVEVWNGLYFGALFPQVDLHALLKPI
                     SDRLSWFPFEKLKFRADVSADYDVKANWALYCDNYLEGFHIPFVHKSLNRVIDYHAYE
                     TILFPGGNLQIGIAKEGELTFNIPPTAEDHGKQIVAYYYWIFPNMMFNFYPWGLSLNI
                     VQPQGKDNTKVSFLIFVADEEKYNTGAGSDLNRVELEDEEVVESVHKGLQSRLYFRGR
                     YSPKMEKGVHQFHQLLETAMHNS"
     gene            9468..10250
                     /locus_tag="PLM90_08920"
     CDS             9468..10250
                     /locus_tag="PLM90_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HMZ89535.1"
                     /translation="MRISLLGEPYSFHHVAATQYWGIDTDILFLDDFDEVIKAVQNGL
                     ADHCIIAVENTVAGEVPGNYKRVVNSGLHIEGEINLHLAMHLAAKQVVPVETLHAVIS
                     HPMALKETAAFFLNYPSIKRISASSTSAAVKTVAESDDEGMAAIGNTSAIRFYNLQII
                     VKNIDNLPNNITRFLILSKQYSNPDATSSPVKASVKLLSASPALPVSIGLRRDFDDRI
                     TYVELPEGPLALILEQIDRLRAAAEDVTLLGVYPTGETASGS"
     gene            10274..11389
                     /locus_tag="PLM90_08925"
     CDS             10274..11389
                     /locus_tag="PLM90_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HMZ89536.1"
                     /translation="MSKELKFKPIEEWGFNFESPLIMAGPCSVESEEQVMETALQLKD
                     QRFDILRGGIWKPRTRPNSFEGIGSQGLKWLKDAGNAIGKPVATEVANVKHVYESLRM
                     GIDVLWIGARTTVNPFAVQEICDALQGVDIPVLVKNPINPDLELWIGAFERLNRAGIT
                     KLGAIHRGFSTHEKTKYRNIPRWNIPIELKRRFPGLPLICDPSHICGTREYLREVAQT
                     AMDIGFDGIHLESHINPDKALSDAKQQLTPAAFGELINSLQMRQSLEQNPYAENQLEM
                     LRGRIDHLDHYLIEMLSERMDVVREIAEYKREHNLAIVQPSRWTEILEDRVNSGQRKN
                     LTEKFIKELFHAMHKESIHHQTEVMLQPDGNKVEKNA"
     gene            complement(11491..>12720)
                     /locus_tag="PLM90_08930"
     CDS             complement(11491..>12720)
                     /locus_tag="PLM90_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89537.1"
                     /translation="VDLFRNNTRINIKKIFCEIKFTDATPLYLPKNTEVLSMEIKFIV
                     EMNFTPDEMTSVMAFLTMPAINAGIGTKETYSMYSLGYEKNWSGRLEALYIKNDIFNA
                     TPIMPTKFSEYTTYIDGDRNQVLKYEDIMLMDKDKMAFYNINNAASCTTGTSYTEQLF
                     IPSAIKNITASSWVKTDQEYAKRAFKQTPMIAFSDSLHPYQTGNPTVVDALHSSSMKA
                     KLYDQSNLYNFIAAGCKNNTPSITFKESGNPLYAFDISDHSYEDSTYDGREHLSRQTC
                     WCEGVNGPGVGEYIEFELTQPSHEMHIYNGNQSSKKAFDESSKADIIVVTSLDGHKIN
                     PNGDNPSVYRSSIIDLTILNVYELAMPAGKYRITIDAVDKGTVPTTCLSSIGFDFQVM
                     DEWYMRSQSMLRSFFKK"
BASE COUNT         3932 a         2565 c         2657 g         3566 t
ORIGIN      
        1 aaagaggctt aattttatgc caacgtttag gggcggcctt ccctccggaa acgggattaa
       61 taattacaaa ccaatcgtac tgcatagtgt atgttaacca atgaaacttt tcgcaatcag
      121 gcacatcagg tggttgactg gattgccgat tacctcgaaa atatacagca atatcctgta
      181 aaaccatcca ttgcccccgg tgcattacgt gcggcgttgc ctgatgaggc gccgcagtca
      241 ggtgaagcga tggatgatat ctggaaagat tttctaaaca tgattgttcc cggaataacc
      301 cattggcaac atcccggatt tatggccctt tttccaagca atgccagcta tccgggcata
      361 ttgggagaga tcgccgtttc aggattagga attaatgcca tgatgtggga cacctcacct
      421 tcggcaactg agttagaaca aaccgtaatg gagtggctga gaaaagctac cggactccct
      481 gacggcatgc ttggcgttat tcatgatacg gcatcaatga gcacacttac tgcaattatt
      541 tcggcacgag aacgctatgc acaatttcag gtgaaccaac acggatttca tggatacgac
      601 aagctggtga tgtattgcac agatcaaacg cattattcta ttgagaaagg cgcaaaagct
      661 gccggaattg gtgccgcgca ggttaaaaaa attgccacag atgatgcaca tgcaatgatc
      721 gccacagcat taacaaaaca aattgcagaa gataaagcag caggactaca accattcatg
      781 gtagttgcaa cactgggcac aaccagcacc ttagcctttg acccgctaga ggaaattggc
      841 aatatctgtg ttgaacataa attatggtta catgtagatg ctgcttatgc aggatcagca
      901 ttttttttga atgaatatcg acattttcta caaggaatag accatgccga cagttacgta
      961 ttcaacgcac ataaatggtt atttacaaat tttgattgct cttgttattt tgtaaaagat
     1021 gccaatcact tgatcaatac gttttctgca aatcctgatt atcttaaacg cacggaagaa
     1081 agtgtggcta tgtttaaaga ttggggcatt ccacttggca gaagattcag ggccttaaaa
     1141 ctctggtttg tgttgcgcaa ttatggtatt tcagggatac aggatttatt gcgaaagcat
     1201 attgatatcg caacaaattt ccgcaatcaa ttattatccg acacacaatg ggagttgatg
     1261 gctccacctg ttatgaatat gttttgcgtt cggtatcacc cacaaggaat caatgataat
     1321 gaagccctaa atgcattaaa taccgcctta ttaaaatcca tcaatgccga tggcaattac
     1381 tatttgtcag gcaccatcgt taaaggtatg tatatgatca gagtagtaat ggggcaaaca
     1441 catctggaac aagttcatgc tgaaggttta ctggctttaa tgagatctag tgcaaatagc
     1501 atcaatgatt ttaaagcatt acgctaacta caaagaagcc agattctgat atacgctatc
     1561 tcttgcctga ataagtgctg ctttttcatg ctcaggaagg cgtttgaagc gcagcgccga
     1621 caaccacttt ttgaagtcga aataataata gtatgcaata tttccggaaa tagggaatga
     1681 gaacaagaat attaatccgg aaacaatact acctaaaaca gtccaggtga tgataaataa
     1741 tatgaggtaa taaatcggaa acaaaaataa actgataaca tacttcgctg aagaccaaaa
     1801 tattttctgc tttatttttt tctctacaaa ttgctgcgga aatttataag gcagataatt
     1861 gatgattgca ccataaatat aaaccggaaa catcaataaa aaatacaaag ccataaacac
     1921 catgctgaaa gtgtggcttc tggatttatc gaatgtattt tctttaaggt tatgctgatt
     1981 gcgtatacgc ttgtagttat gtaagttttg aaccaagcgt ttcatccctt catgatcact
     2041 atgctgataa gcatccaact ttgcagagat cactttctct tcattaaaag aatttagtgg
     2101 tgtaaccgga atattgttgt taagcagcgt ttctctggcg tgcattttac tcaattcaac
     2161 aatatcgtga tgaaaagttc cgtgaggcac atgcgtagct gtttccttca tagccttttc
     2221 gatatcatct ttcaggatat tgattgtttg agcaggtgca ctgcggtatt gctcataata
     2281 ttcctttaac aaaataggtt tgccaaaatt caatagctgc gtatgtttga atttttcggc
     2341 acccgaatac atcactccaa cagggatgat atgtatatca ggttgaaatt gatatttttc
     2401 agcggcaaga aaagcaatac gacaaatacc ttttttcagc ggacgaatat gttcttcatt
     2461 tatacaattc ccttcaggat agatccccac catgccattg ttgtaaagaa tatcggcaca
     2521 ggcttcaaag gtaggttggt tcatgttttt ggtatccacg ccatcccgtt cacgccacaa
     2581 tggaagcatg tgaaagaaga taaacaactt cttcatcata ggattattga aggcatcacc
     2641 tcttgctaat tgccatacct gagttccttt gcaattcaca aatgccaaag aatccataaa
     2701 tgcattgcgg tggctgattg caaatattac aggcttatcg gtaggaacct gttcccatcc
     2761 cgccacttca tattttgaat agtaatattt ataaggaatg ccaatcaaga atggctgcac
     2821 agtccagtaa cctttggtat aggtgtgcaa agtggtatcc ctgaacggga taggattctg
     2881 tttctcaaat aaacgcctga gcagccgctt gtccatagta aaacaggtgc aaaaataacc
     2941 gttttaagct tgcaatgtct ttttcgctgc cattcgcgac caataagtag ctatgatgaa
     3001 gcctgtaact aggtcccaga tgccccacca accggctact actgccattc caccaagtcc
     3061 tttaaaaaag ctgaaaatga tgatcagacc aagtgcagca ttgtgaattc ctgtttcaat
     3121 tgataaacac tttttatcgg caaaatcgag tttaaatagt gctccgaggc catatccacc
     3181 taacattgct aatccgttat gcaagagcac cagcaggaaa atcataccaa taatttccat
     3241 gaataaatca tagttcttca gaaaggcaaa tacaataaag caaaggaaga tgatgacaga
     3301 aacaatttga acaggcctac ggatcaatgc tgtgaatttt ggcataaaat gatttaccaa
     3361 catcccaata ataagtggaa caccaaggat cacaacaatg gtggtaaata attccatagg
     3421 ttcgacactt atttcctgca tcaattcagc agttggaggg tataacccac cataaaatgc
     3481 aaaattcagc ggcgtcataa ttggtgccaa caaagttgag gttgctgtaa gcgttactga
     3541 aagagcaaca ttcccttttg ccagtgagga aaggaaattg ctcatattcc caccgggaca
     3601 tgctccaaga agaaaaatac ctaaagcaat actcggacaa ggctgtagga tgattacaat
     3661 caaataggta agaaatggca gcaacacata ctgggataga attcccgcaa tggcctttcg
     3721 cgggtattga atcagacgtt taaagtgttc aacctttagg tcaagtgcta ttccaaacat
     3781 cagaaatgca agcagacaat taagcagaat cagattgttc tgatcaaaat ttaattgctt
     3841 tgcgtcaatg atatcacaca tatgcctggt tttcgacgca agatagtatt tcttctacgt
     3901 gtatgaaaat ggatcaatag ccgtaatttt gtgcaaaaat caagggtgag gatatttccg
     3961 gcggacatac cacaagtatt ggaatttgaa aaggtgttac acgaggtagc caggtattgc
     4021 gatagcagtc cgggggcgga tcgggttgca agagtgcgtc cattggacaa atttgatgag
     4081 gtgaaaaaga tgttgttgca aacacgcgaa atgacgcatt tgctgcaata tgcccaagga
     4141 ttccctcacg ataaaatgtt tgatatccgc cctaccctac cactgcttgc catagaaaac
     4201 tatgtattaa gtgaacagca atgccatgaa atacatcgcg tggcgctatt gatgggtagt
     4261 atcatccgat ttttcaatag caatcaggag caatatccgg tattgcagca atttgtcagc
     4321 ggcacaactt atgtaagcgc tatttctcaa tcgataaaag cagttattga tgatgcaggg
     4381 catatgcgca atgacgcgtc gccagaactt gtgcgcattc tgaaagaaaa agacgatgcc
     4441 atgcgttcgc ttggaaaagt gtttcagtcg gcactacaaa aatatcgcag tgccggattt
     4501 ttgagcgagg tggaagagag tatgcgcaat ggaaggcgag tgttaggtat tcaaagtgaa
     4561 tacaagcgca aaattgccgg tatcatacat gatgaaagcg aaaccggtcg tacggtattt
     4621 atggaaccac aggaagtggt caatattcaa aatgagattt tcgaacttga acgggaagaa
     4681 aaacgcgaga tctatcgcat tttgaaaacc ctcacggcag acctggcgcc acatcaacaa
     4741 atactggata aatatcaaca gatacttggc attatagatt tcaccagggc gaagggccgt
     4801 tatgctttgg aaacacaatc aagcatgccg caattagaaa aacaacccat tgtttgttta
     4861 tacgatgcac gtcatcccgt attattatcg ctgcataaaa aacaacataa acaggttgtc
     4921 cccttgcagg cagagcttga tagcaataat cgcatattag tgatttcagg tccaaatgca
     4981 ggtggtaaat ctgttgccat gaaaacgatt ggtttattgc aatacatgac ccaatgcggg
     5041 ctgctgatac ctgcttccag ccattcaaga attggcattt tcaaacaaat attttgcgat
     5101 atcggcgaca cccaatcgct ggaagatgaa ctttctacct attctgcccg cttgatgaag
     5161 atcaggcatt tccttcaaca cgcagaccgt gaaaccttat tattgataga tgaatttggc
     5221 agcggcactg atcctgtgat gggtggagcc attgcagaag cagctctgcg tatgttgaat
     5281 gaaaagaaaa ttttcggcgt gatcactacg cattatgcca atctgaaatc gtttgctgca
     5341 aatgccatcg gcttgtttaa tggcagtatg ttgtatgatg aggcagaatt aaaacccatg
     5401 tacttgcttg aaacaggtaa acccggcagt agttacgctt ttgaattggc aaagaaatct
     5461 gccctacccg ggactttgat agaggaggca aagaaactga tcagtaaaga acatattcga
     5521 ttcgaagagt tactgaaaaa tgtacgtata gaaaaagaac atatcagatt acgtgacaag
     5581 gaggtttcaa aaaaagagga gcaattaaaa aagaaggaag ttgaactggc tgccgcgatg
     5641 gaaaaaacgc gcgagcaacg tgaaaaatat aatcttaaaa aactggagaa agaggatcaa
     5701 accattaggg aaatggaagc cacgtttaaa aacttgttac aagagtttaa gcaagtgcaa
     5761 tcagatcctg aaatggataa tgcaaccaaa gaacaatcca aagatacggt gcgtaaattc
     5821 tttcaggagc aacgtaaaaa gacatttgat catcagaaaa aaatacgccc tactccaatt
     5881 ccgcaatggc agcgcggtga aataaagatc ggcgggtatg tgaaattgaa ttcgggaagt
     5941 gaaatcggag tattggaatc tgtaaaaaat aataaggcta ccgttatatt caacaatatc
     6001 cgcacaacag ttccttatgc cgatttaatt ggcattgcaa aaacagaagc tcctgctatt
     6061 acgcctgcaa aaattaaggt taccattgat gaaagcgatt tcaatacgga attggactta
     6121 cgcggacaac cacgcgaaga ggcaatgatg aatctcgaac agtatctgga tgctgccatg
     6181 atgcgcaaga tttatcagtt aagaatattg catggcaaag gcacaggcgc catcagagag
     6241 gttgtgcagc aaacattgaa atcgcatcct gccgtgttgc gtttcgaatt tgcaaacaga
     6301 gaaggtggcg gcgacggagt gacacttgtt accttagggt aaacgacact cattaaaatg
     6361 tcgtaatttt cagatgtaaa gcctaaacta tgcgcaatct tctcctctta ggtatcattt
     6421 ttatacatgc aagtttatcc gcacaatgct tcataggtat agacagcata tattgcacca
     6481 acggcattcc ggatactatt ttacctgttg ataccggagg atattttgat ggggatggta
     6541 ttagtgcaaa tggaattttc tttcctgaaa tactggatcc cggttcttat gtaatcagtt
     6601 ataagaatac tcagtattac tatgttgata ctacaggcgt ttttgcaccg cttagtggtg
     6661 atgaacaata tgtttcatta atagatgatg gatttagtga tggaatagaa ataggatttg
     6721 attttgaatt tttcggtagt ttgaaaaact atgtccgcat ccacagcaat ggtttcatca
     6781 cgttcaattt cgaccttgcc atcttcccac cctatgcaaa tgatatcccg actcccggta
     6841 tgccaacaga tctgatagct gcttcgtggg gcgattacga ccctacaatg ggcggcacaa
     6901 tttcctggtt tcagtcaggc tccgctccta atcgtaaact tgtgattaat tatcgcgacc
     6961 tacctcccta tggcgaaacg gagggactgc ttacaacgca agtaattctt tttgaaacaa
     7021 ccaatattat cgaaatacat accactgcga gtcccgacta tggcaaacgc aaaacacaag
     7081 gcatagaaaa tatcgacgga acagcagggt atgtcagacc cggcagaaac aatagttact
     7141 gggaaacgta caacgattat gttgcattta ttccacagtt ctgtattgat acgctatatg
     7201 tggaagatcc tccggtaacc gaagttttag atgacgaaat caatatttgc atgccatacg
     7261 cgggtttatt acatgttagc ggtgctgata cttacttatg gctgtctgat gatatatgca
     7321 cgatgacccc cggtcctgat cccgattcat attatttaca cggtattgct gaaggcacct
     7381 ttacttgtaa tattgtcggg ttcaatgaag caggatgttc agatacatcg caagtagttg
     7441 tcaatattga aacatgtcag acagctatta caaataatgt cgaaacacca tttcagatta
     7501 cgccaaatcc tgcaaataat atcttaacaa ttattatagc agcaaatgaa tttaacggca
     7561 aatggtcgct ggttaattta caaggtgctg aaatgcaatt tggggaatta accggattga
     7621 aaactgaaat cgatattcgc gaattgcctg caggaatcta ctttctgata tttgacgcag
     7681 gcgattgttc ggcgagggaa cagttcatta tttcgcatta acattttttg tgcatttatt
     7741 gtgcattcac gctgaccact ccttccaaaa tccctattct gactgcattt caaggctttt
     7801 agcttatcca ccatttgtgc ataaaccctg tgcaaatgct gtgcgtaaaa atatgccaat
     7861 ctctattggc ttgaatgctg ttacaagata tatttggaat acctgatgac tatccggtgc
     7921 cgttagcagt agcggacgag gacaaccgaa aggaagttcc ggaaagcgca tcctgaaaag
     7981 gaaagctata aggtgccacg caagtgagac acccggaagc gaaatcagat gtatcagcag
     8041 taactacggt tattgatgat gcgaaagcgg agtcggcgag aaaaagttga ttccgctttc
     8101 ttcgtaagat aattgacgga attcgtgtag cgctaatttc gtcgatgaaa aaaaacctcg
     8161 ccggtcatgc gaggttttta tattaaaaga ctttacgaat tgtgcattgc tgtttccagt
     8221 aattgatgaa attgatgcac acctttttcc attttcggcg aataacgacc tctgaaatac
     8281 aatcgcgatt gcaaaccttt gtgcacactt tcaactactt cttcatcttc cagttctacc
     8341 ctattgagat cgcttcctgc accggtgttg tatttttctt catcggcaac aaaaattaaa
     8401 aacgacactt ttgtattgtc cttgccctga ggttgcacga tgttgagcga taatccccat
     8461 ggataaaaat tgaacatcat attcgggaat atccaataat agtaggccac aatttgtttg
     8521 ccatgatctt cggcagttgg tggaatatta aaggtaagtt ctccttcttt tgcaattcct
     8581 atctgtaaat tacctcccgg gaataatatc gtttcatatg catgataatc aattaccctg
     8641 ttcaacgatt tatgcacgaa aggaatatga aatccctcca gataattatc acaatacaat
     8701 gcccagttag ctttaacatc atagtcggcc gatacatctg cacggaattt caacttttca
     8761 aatggaaacc atgagaggcg gtcggaaatt ggtttaagca atgcatgcaa atcgacttgc
     8821 ggaaataagg ctccgaaata cagaccattc cacacttcta ccttagcttt tgccaggtca
     8881 tccatgcgcg ttggaaaatt ctccgcttcc tcaaattcag gcatactcat aaatgctccg
     8941 tcaggatgga aacacctgcc atgatatttg caccggatga cctgcgattt tgttttctct
     9001 gtcaataaaa catatccgcg atgagtacac acattcgaca tcaactgtaa aacatcatcc
     9061 tgcacactca atatcaatgg ctcattcaaa caaccgggaa acaggtttac gggatataga
     9121 tatcctgttt cctgaaattg atgtttatcg ccgatataat gccaactgcg cgtgaatact
     9181 ttttccctga caagcgcata ttgatcctcg ccgaggtaaa aatcaccggg aggggttgca
     9241 gcgatgcgga tatttttatc tatcatctca caaaattaat tatggcaaat tcgccttcct
     9301 tgctgatatt gttgaaaaac aaggtgatgc cgcgcaatta aaatttacaa aatcaattga
     9361 aaatagtgct ctgctttatc ggtatccgct taactatcat cgttaaagta tgtgcaaatt
     9421 tcaagcccgg agaagcgatt aagacctgtt ttaagtaatt ttgcgtcatg cgcatcagtc
     9481 tactcggaga gccgtattct tttcaccacg ttgccgccac gcaatattgg ggaatcgata
     9541 cggatatatt attccttgat gattttgatg aggttatcaa ggctgtccaa aacgggctag
     9601 ccgaccattg cataatagcc gttgagaata cggtagccgg tgaagtaccc ggaaattata
     9661 agcgggtggt gaacagcgga ctacatattg aaggtgaaat caacctgcat ctggcaatgc
     9721 atttagctgc caaacaggta gttcctgtgg aaacattaca cgcagtcatt tcccatccca
     9781 tggcattgaa ggagaccgca gctttttttc ttaactatcc ttcgataaaa cgcatatcgg
     9841 cttcttccac ctcagcggca gttaaaacag tggccgaatc tgacgatgaa ggcatggctg
     9901 ccattgggaa tacttctgcc atcagatttt ataatcttca aataattgtt aagaatattg
     9961 acaatttgcc aaataatatc acaagatttt taattttgtc taagcaatat agcaatccgg
    10021 atgcaacctc ctcacctgta aaagcgtctg taaagctact gtcggcaagt ccagcattac
    10081 cggtttcaat tggtttgagg cgggattttg acgaccgcat tacgtatgtt gagctcccgg
    10141 aaggtccgtt ggcattgatt ttggagcaga ttgaccgatt gagagctgcc gcagaggatg
    10201 taaccttact tggggtatat cctaccggcg aaacagcaag tggatcctag agttaacaca
    10261 aaatattaca agcatgagca aagaacttaa gtttaaaccc atcgaagaat ggggtttcaa
    10321 tttcgaatca ccgctgatca tggcaggtcc ttgcagcgtg gaaagtgagg aacaggtaat
    10381 ggagaccgct ctccaattga aagaccagcg ttttgatata ctccgtggag gtatctggaa
    10441 gcctcgtaca agaccaaatt catttgaagg cattggcagt caagggctga aatggctaaa
    10501 agatgccggc aatgccattg gaaagccggt agcaacagag gttgcaaatg tgaagcacgt
    10561 ttatgaatcg ttgcgcatgg gtattgatgt gctttggatc ggtgcacgca ccacagtgaa
    10621 cccatttgct gtacaagaaa tctgtgatgc tctgcagggt gtcgatattc ctgtattggt
    10681 aaaaaatcct attaaccctg atcttgaatt atggataggc gcatttgagc gactaaacag
    10741 agccggaatt accaaattag gagccataca ccgcggcttt tctactcatg aaaaaacgaa
    10801 gtatcgcaat attcccaggt ggaacattcc gattgaactg aaacgtcgtt ttccgggatt
    10861 acctctaatt tgtgacccaa gccatatttg tggcaccaga gaatatttgc gtgaggtagc
    10921 ccaaacagca atggatattg gtttcgatgg cattcacctt gaatcgcata tcaatcccga
    10981 taaagcactc agcgatgcca aacaacaatt aacacctgct gcatttggtg agctcattaa
    11041 ttcactgcaa atgcgtcaat cgctcgagca aaatccatat gctgaaaatc agttggaaat
    11101 gctccgtggc cgtattgatc atcttgatca ctatttgatt gagatgttaa gtgaacgtat
    11161 ggatgtagtc cgtgaaattg ctgaatataa acgtgaacac aatcttgcca tcgtgcaacc
    11221 tagtcgctgg acagaaatat tggaagatcg cgttaattcc ggtcaacgca aaaatttgac
    11281 agaaaaattc atcaaagaat tattccacgc aatgcataaa gaatccatac atcatcaaac
    11341 agaagtaatg cttcaacctg atggcaacaa agtggaaaaa aacgcataaa cccaagtaaa
    11401 cagattatgg aatcccgaca gcaatgccgg gattttttat gcataaaaaa acccgatgtt
    11461 gtggcatcgg gtttttatgt ttaagcgtta ttatttcttg aagaagctac gcagcatact
    11521 ttgcgaacgc atgtaccatt catccataac ttgaaagtcg aagccgatag atgataagca
    11581 ggttgtaggc accgtacctt tatcgacagc gtctatagta atgcgatatt ttcctgccgg
    11641 cattgccaat tcatacacat tgaggatggt gaggtcaatg attgaactgc gataaaccga
    11701 aggattatca ccgttcggat ttattttatg tccatcgaga ctggttacta ctattatatc
    11761 agcttttgaa ctttcatcga atgctttttt cgacgattga ttgccgttat aaatgtgcat
    11821 ttcgtgtgat ggctgagtaa gctcaaactc aatgtattcg ccaactccgg ggccatttac
    11881 gccttcacac caacaggtct gacgtgatag gtgttcgcga ccatcatagg tactatcttc
    11941 ataagaatga tcagatatat cgaaagcata taatggattt cccgactctt tgaatgtgat
    12001 tgaaggtgta ttgttcttgc agccggcggc tatgaaatta tagaggttag actgatcgta
    12061 taatttcgcc ttcattgagc tgctgtgtaa tgcatcaact actgtaggat ttccggtttg
    12121 gtatggatgc aatgaatctg aaaaagcgat catcggcgtt tgcttaaatg cgcgcttagc
    12181 atattcctga tccgttttca cccaactgct ggctgtaata ttttttatgg cagatggaat
    12241 gaatagctgt tcagtatatg aagtacccgt ggtgcaactt gctgcattgt ttatattata
    12301 aaatgccatc ttgtccttat ccatcagcat gatatcttca tacttcaaca cctgattgcg
    12361 atctccatct atataggtgg tatactccga aaattttgtc ggcataattg gtgttgcatt
    12421 gaaaatatca tttttaatat acaatgcttc gaggcgacca gaccaatttt tttcgtaacc
    12481 taatgaatac attgaatagg tttcttttgt gccgataccc gcattaattg ccggcatggt
    12541 aagaaatgcc ataacactag tcatctcatc cggtgtaaaa ttcatttcaa caataaactt
    12601 tatctccata gacaaaacct ctgtattctt tggaaggtaa agtggtgttg catccgtgaa
    12661 ttttatttca caaaatatct ttttgatgtt gatgcgggta ttatttctga ataaatcaac
//
LOCUS       DAOQOU010000091        12591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2568_length_12591_cov_6.026085, whole genome shotgun sequence.
ACCESSION   DAOQOU010000091 DAOQOU010000000
VERSION     DAOQOU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12591
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_12591_cov_6.026085"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(420..1184)
                     /locus_tag="PLM90_08935"
     CDS             complement(420..1184)
                     /locus_tag="PLM90_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89538.1"
                     /translation="MKKIYAFFTALLITGALTAQVNVTYMVDITNYLAAGNVLGANGI
                     RVGGDFGATAGMNGAAAMAVWTPSDPTCALVDMGGNVWGITVTYPTASVGLTQYYKFV
                     NNDWGTNEGTDAGNTIGADGCGVDDGAGNVNRTLVIPDADIMYLFCWDACYRCDGSDP
                     VVLSVEDFNAISGLSVSPNPTVTNTNINFNLSEASDVTIGVYNLMGQEVLTVNNGTQS
                     AGNHSYSLDMSNLPAGNYVYHVVAGSSVTSGNIVKL"
     gene            complement(1304..2491)
                     /locus_tag="PLM90_08940"
     CDS             complement(1304..2491)
                     /locus_tag="PLM90_08940"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMZ89539.1"
                     /translation="MQAQMSVIDQAIVDITSVIRHDVFSAPAASRIYAYTTIAGNIGM
                     SGNSDLPTPLRSKLVLSSGVSPSDTLAAMAMYVCAQRFIYSDSMLLEKCKKRLGSEII
                     TSQYFMEAQTINASMADWINADNYTNAIASTRYTPLKGSLNWVPTPPDYAQGTDPHWG
                     EIMPFCYKYDKSILPVPAFVLDSTVIAKEAQKLYVLTMSLDSEQMEIALFWDDNPFIL
                     RQNGHLQYAEKKVSPPGHWLDITRMALLATGADETAAMNAYMLVSIAMADAMIAVWDI
                     KYETQLIRPETYINTYIDSEWRPYLQTPPFPEFPSGHSAVSAAAATILTSLFGDNFAF
                     TDATETTFGLPARGFKSFEDAASEASQSRVYGGIHFVPAVTYGAELGKNSAFELINIT
                     NRR"
     gene            2771..3664
                     /locus_tag="PLM90_08945"
     CDS             2771..3664
                     /locus_tag="PLM90_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89540.1"
                     /translation="MNSHITHAQITTDITPVPGLINFQNLRDYCLNASGDEDFFTIQS
                     PNGEISQIATRKKINGNWSSPELMPFCDAFMYLEPFLTTNGLTLYFVSDRPLNDGDTI
                     KKDFDIWMVKRAAITAAWSKPVNLGTPVNSTNDEFYPSLTTSGDLYFTMAAPEGMGKD
                     DIYMCKMTSTGYEKPTILPSPINSAGYEFNAFISPDGNILIYTKYNAPGGFGSGDLYI
                     AKKDNGGNWQEPQNMGTDLNSMYMEYCPFYHYPTATLYYTSRRCNLTPKTFNDVNELQ
                     QYISGNENGNSRLYYRKMKIE"
     gene            complement(3865..5985)
                     /locus_tag="PLM90_08950"
     CDS             complement(3865..5985)
                     /locus_tag="PLM90_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ89541.1"
                     /translation="MKKPQIWIALLFLALSSCSDHTEYTKVNTRFSTTDPFAKTSAPS
                     KFVEFSAEIDTVLAGENGLIIVIPKGALLNAKNEVVQGKINMELFQANGLNELVFSNL
                     TGNSSSGQLSGGSAFYINFTKDNEQLHIDQNNPITISTPKTAKEMLMYSGNRDSVGNM
                     DWVDPIAPEKYLDPVPLSTLNFTPEIFDNGLLDILPFEGKNKFVRSFSDSLYYSVWLV
                     HKKLSSFTPDQGEKFLLEPNGKKFDLKDIQKSHQSGLTDTSSDDEYSDCGIDPIKVKT
                     IKENAFENTFIATKEFETRMQAIHRTFDQRVLDLYVNNTDKQLWVVDSMASQLLGNAA
                     DREVFLKFSREKLTNVRDVEKNPSAKALARFYQKRSEGLTREMDTVENALLKKYKLLQ
                     KDYDKELAKYVEVLRKREAHRMLYFNYTLTSSGWKYFAIPPEFGRTFREKKLIGRLTD
                     SQKFDMVNVYVWMPENTSLMRLTETEKNVFISKSMRAVETTPVLDNTKLQFFAVAVIG
                     EQYFFDEVKLSSEEEREVTATFELKPTEKAKIDAVLGNQEGKADLNNSISRDLKFQTK
                     RLAFNKAADKYLEKRTKYDLLFSTTVNNCCRMSSKSIQVGNALFNVNCTRCHLAERGV
                     KLTGPAFDNLLDHSSGQYAYFSAFVRDSRALINSGDDRATTVFNEYRSVMPAFEDLKE
                     EEIMSIYNYLIATLSCSNQPTILH"
     gene            complement(6048..9335)
                     /locus_tag="PLM90_08955"
     CDS             complement(6048..9335)
                     /locus_tag="PLM90_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMZ89542.1"
                     /translation="MNRKFVAYWIVSALVAYSPTFAQTTLFSRLSASQTGVAMHLDLQ
                     ETATMNVLLYQYLYNGGGVAAGDVDGNGYCDLFFTGNLGQDKLYLNQGNMQFVDATRQ
                     ANILPPGGWSSGVTMADVNGDGFLDIYVCKSGQFSTRDRHNQLYINNGKGAFVESAKK
                     FGLDDSSFSTQAAFFDMDNDGDLDMFLLNHAVTQLHGFNIGDVRNRRDKYAGNKLFRN
                     DNNYFTDISAEAGIFGTPSNFGLGLMIGDVNNDAYLDIFVTNDYNEQDFLYINNHNGT
                     FSQVLQDAFGHTSNFSMGCDMADINNDGWLDLLVMDMLPEDPERQKKLKGPTKFDAYQ
                     MSADYGYFYQHMFNTLQINNGNNSFSEIAWSAGVAATDWSWAPLFADYDLDGIQDLYI
                     TNGFRHDFTNLDFLKYTYSEAEQKAFDAGEKLNTLDLVYQMPDVPLSNYCFKGSDSLQ
                     FKNVTVDWGVQIPSYSNGAAYADLDNDGDLDIVVSNIDDTVFIFKNNARELKQNHYLK
                     IKIIGEGKNTFGVGTMISVAIGNQSYKRQLMPTRGFQSAVEPVLYFGLGESKNVDRIQ
                     IIYPSGKEQILSNISADTTLIVYEKNGQYELWAADYLPEFIDVSNKVIPYSVSEEPAD
                     DFKKEPLIPYVYSDQGPALAVGDANNDGISDVFITGGKNKKSRLFLGSDEGFIPSFGG
                     VWERDSAYEDVDAAWIDVDNDGDSDLFVVSGGNEYPAGSRYYQSRLYLNMSNGRYVNA
                     NQLLPESLQAHSVVRTCDFDNDGDMDIFLGGGVVAGSYPLSSPSAIYENIGGKFMDIT
                     ARICPELNTIGLVQDAQWADIDADGKQDLVVVSHWSPLRVFINGKKKFIESTVPSGLS
                     DYSGWWNAINIVDVDLDGDMDIIAGNRGTNHPLKASKEFPAHMYVCDFDRNGKIDPII
                     TTFDKSGKEIPIASKDDMLEQIPALKNRYVYYADYAKASIFDLFPDVNWDTITHFTAT
                     DFNSSIFINNGKGSFTRQLLPEQIQWFPVYAIAVKDINRDNYPDLILAGNQFGTRPEL
                     TRLDAGYGACLFGDGKGGFTFKNAAETGLFIDGEVRSMCLTKLNREKYLLVARRNASV
                     MAYLLNK"
     gene            9561..12512
                     /locus_tag="PLM90_08960"
     CDS             9561..12512
                     /locus_tag="PLM90_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ89543.1"
                     /translation="MMHVYALIRRAILPVVLLMPVLIWGQAPTLTGVISDQSGATLPG
                     VTVLISGTGKGTISDFDGSYVLAFDAAGTYTVNYTYIGYKAVTETITLTDGQNLKKDL
                     IMQEDALMLETAVVVGYGTIKSKDLTGSITNVGVKDFQGGNVTTPEQLITGKVAGVQI
                     TSNSGMPGSGSRIRIRGGTSLNASNDPLIVIDGVPVDNNGISGSGNALSLINPDDIEN
                     ITVLKDASAAAIYGSRAANGVIIITTKKGFSDNKLHIDVSSTSSLSQVTGYVDVLTAD
                     QLAAVLDTAGTATQQALLGSENTDWQKELYRMGFAEDLDLTFTGGIKNLPYRLNLEYM
                     NESGVLDRSQLGRMGASLGINPTLLNGKLKTEANGKYYHTDNFFADQGAIGSAITFDP
                     SQAIYDDASVYGGYWEWTNTSGGLIPLAPKNPVGLLNQRDDESNVNRFIGNIKLDYEI
                     FKDFHGVINGGIDMARSNGTVVVPAEAASQYPNGSNNQYEQSKDNKLLEAYGNYKKDI
                     KSIKSRIDFTAGYSYQNWLTQSPSFPSLSFAGDTITPVGIDFETENTLISFYGRLNYN
                     LKERYMVTATLREDGSSRFSPDNRWGLFPSVAAAWLVSDEAFMSNTKMYLKLRAGYGV
                     TGQQDIFYDYPYIANYDSSTSTAQYQFGDAFYYLLRPDGYDPNIKWEETTSFNIGFDF
                     GLKGDKISGSVDLYKKLTNDLLATIPIPAGTNFTNQILTNVGSMENTGVEINLSYIAL
                     DNKDMSLEFGINGTANRNEITNLTLVEDTSSAGILVGGIAGGIGNTIQIQSVGYASNT
                     FFAYQQQYDENGNPIEGSYVDQNGDSIINADDLIWGENPTPDFYLGFSAAFKYKKWSV
                     STVLRGEFGKYMYNNINSTRGIFQNVPSASYMQNLSTNYLETGFQSYQLLSDYYIENA
                     SFVRMDNFAVGYDVGRVFNDNASLTVGAVIQNVFVISPYSGLDPEIAGGIDNSIYPRP
                     RIYSLNINLKL"
BASE COUNT         3657 a         2713 c         2528 g         3693 t
ORIGIN      
        1 caaattagca agtggtggtg cttttaacgc tggcgcgtta atagggaatg caattcaatt
       61 gctaaataaa atttaattct gaaacgcaat tagctccctc tctatcgacg gtaggagatg
      121 gagagggcgg ggggtgaggc ggggccgggg gtgaggcggt ttcgcgcaaa gtcgcaaaga
      181 gactaaggtg caaaagtcta gatactagat acttgattct agatactaaa agagagttag
      241 catgtgttca aaggattcat tttcgcaaag tcgagtcaag attaccaccc gcctactcgg
      301 cgcggtaaac tcacccactg acccactcac ccactctctc ctttttcctc tctccttttt
      361 cctataaaaa aaacccgcct tcaaacgaag acgggttttt tatttcgttt tgctaattat
      421 tagagcttca cgatgttacc tgaagtaacg cttgaaccgg caacaacatg gtaaacatag
      481 ttacctgcag gaaggttgct catatctaaa gaatatgagt ggttacctgc tgattgagta
      541 ccattgttaa cagtcaatac ttcttgaccc atcaggttgt aaacaccgat agttacatca
      601 gatgcttcgc tgaggttgaa gttgatgtta gtgttggtta cagttgggtt aggtgaaact
      661 gaaagaccgc tgatagcatt gaaatcttca acgcttaata caacaggatc agaaccgtcg
      721 caacggtagc aagcatccca gcagaacaaa tacataatgt cggcatcagg aataaccaat
      781 gtacggttaa cgttacctgc gccatcatca acaccgcaac cgtcagcacc aatagtgttg
      841 cctgcgtcag ttccttcgtt agtaccccag tcgttgttca cgaatttgta gtactgagta
      901 aggccaactg aagctgttgg ataagttaca gtgatacccc atacgttacc acccatgtca
      961 accaatgcac atgttggatc tgaaggagtc catactgcca tagctgcagc accgttcata
     1021 cctgcagtag cgccgaagtc accgccaaca cgaataccgt tggcacccaa tacgttacct
     1081 gctgccaggt agtttgtaat gtcaaccatg taggtcacgt ttacttgtgc tgtaagagca
     1141 ccggtgatca acagagcagt gaaaaaagcg taaatttttt tcataagatc gttgtttgtt
     1201 ttactttaag aaatgaattt gtcaggataa aggtactgct ttttattgca tttgaagcca
     1261 aatttttaac atgaaaatga ccgttgatta atatgttaaa cacttaacgc cggttggtga
     1321 tatttatcaa ttcaaacgct gaattttttc ccaattctgc cccatatgtt actgccggaa
     1381 caaaatgaat tccgccgtaa acgcggctct gagatgcctc agaagcagca tcctcaaagg
     1441 atttgaaccc tcttgccggc aggccgaatg tggtttcagt tgcgtctgta aatgcaaaat
     1501 tgtcgccaaa taatgaagta aggattgttg cagcggcggc ggaaactgcg ctatgcccac
     1561 tcggaaattc ggggaagggt ggggtttgta aatatggcct ccattccgaa tcgatatatg
     1621 tattgatata tgtttcagga cgaatcagtt gcgtttcata tttaatatcc caaacagcaa
     1681 tcatggcatc tgccattgct atactaacca acatatacgc attcatggcg gcagtctcat
     1741 cggcacctgt tgccagaagc gccattctgg ttatgtctaa ccagtgtcca ggcggactca
     1801 cctttttttc agcatattgt aaatgtccgt tctgacgaag aataaatgga ttgtcatccc
     1861 agaatagtgc aatttccatt tgttctgaat cgagagacat tgtcagtaca taaagttttt
     1921 gagcctcttt tgcgataaca gtgctatcta aaacaaacgc cggaacaggt aatatgcttt
     1981 tgtcgtactt ataacaaaac ggcatgattt ctccccaatg tggatctgta ccttgagcat
     2041 aatccggcgg cgtgggcacc cagttgagcg aaccttttaa tggtgtatat ctggttgacg
     2101 caatggcatt agtgtaatta tcggcattaa tccaatcggc catggaagca ttaattgttt
     2161 gcgcttccat aaaatattgc gaggtgataa tttcactccc cagacgcttt ttgcatttct
     2221 ccaatagcat gctgtcggaa taaataaatc gctgtgcaca tacatacatg gccatggcgg
     2281 caagtgtgtc tgaaggagaa actcccgacg ataaaaccaa tttacttcgt aagggtgtgg
     2341 gcagatcaga atttccggac atgccgatat ttccggctat ggttgtataa gcataaatgc
     2401 gagaagctgc aggagctgaa aacacatcgt gccgtattac cgaagtaatg tcgacaatcg
     2461 cctgatcgat cacagacatt tgggcttgca tgcattgggt gaacaacaag catattatta
     2521 tcaggtagcg catttcatgg agaaaggtaa gcaatagttt gatgtgataa aaagctgctg
     2581 caacaattat acctgaattg ctgaaaacgt atctcaataa taataatcaa tcttaatact
     2641 ctttatgact tcgaaagcat actttaacaa cccgcaaaat tgaaagaaat tatttttgaa
     2701 ctgtacctaa ttttcaaatc cacataccct aatgatgaaa aatatttttt ttgcttgctg
     2761 tttttttctg atgaacagcc atattacaca tgcacaaatc actacggata taactccggt
     2821 tccgggatta attaattttc aaaatctgag agattactgc ctgaatgcaa gtggtgatga
     2881 agattttttc accattcaaa gtccaaatgg tgaaatatca cagatagcaa cgaggaaaaa
     2941 aatcaacggc aactggagtt cacctgaatt gatgcctttc tgcgatgctt ttatgtattt
     3001 ggagccattt cttactacta atgggctgac tttgtatttt gtatccgacc gccctttaaa
     3061 cgatggcgac accattaaaa aagattttga catctggatg gtgaagcgcg cggcaattac
     3121 ggctgcctgg tcgaaacccg tgaatctggg cactccggtt aattcgacaa acgatgagtt
     3181 ttatccaagc ctgaccacct ccggcgattt atattttacg atggcagcac cagaaggaat
     3241 gggcaaggat gatatttata tgtgtaaaat gacatcaaca ggctatgaaa aaccaacaat
     3301 attaccatcg ccaataaaca gtgcgggtta cgaattcaat gcatttattt cacccgacgg
     3361 caacatcctg atttatacga agtataatgc tccgggcgga tttggcagtg gcgatttata
     3421 cattgcaaaa aaagacaatg gcggaaattg gcaggaacca caaaatatgg gcacggatct
     3481 caactccatg tacatggaat attgtccttt ttatcactac ccaaccgcta cgctatacta
     3541 tacaagtcgc cgatgtaatc tgacaccaaa aacctttaat gatgtcaacg aattgcagca
     3601 atatatcagc ggcaatgaaa atgggaatag tcggttatac tatcggaaaa tgaaaataga
     3661 atagggatta aaccatctcg tcaaaattaa tttaaactcg gcggatcgga gcacttcaat
     3721 gatctggtta tataattata aatcgacatt aattcttctt cagtcagttc ctgatatgcc
     3781 ggcattaccg attggtattc gttgtataca ttattggcgc gctggttgcc gctagctatc
     3841 tgtgcttgtg aattgcgaac aaactcaatg aagtatagtc ggctgattgc tacaggataa
     3901 tgtggctata agataattat aaattgacat gatttcttct tccttcagat cctcaaatgc
     3961 gggcatcacg gatctatatt cattaaatac agtagttgcc cgatcatcac cgctattgat
     4021 aagcgctcgt gaatcacgaa caaaggcgct gaaataagcg tattgtcccg atgaatgatc
     4081 caaaaggtta tcaaatgccg gtccggttaa cttaacacct cgttcagcca agtggcacct
     4141 ggtacaattc acattaaaaa gggcgttacc aacttgtata gattttgaag acatcctgca
     4201 acaattgttc accgttgtag aaaacaagag atcatacttg gttcttttct ccagatactt
     4261 atcagcggct ttgttgaatg caagacgctt ggtttgaaat ttaagatcac gagaaattga
     4321 gttattgagg tctgccttac cttcttgatt gcctagcact gcgtcgatct tcgctttttc
     4381 tgttggtttg agttcgaagg tggcggtgac ctccctttct tcttcggacg acaactttac
     4441 ttcatcaaaa aaatactgct cgccaattac ggctaccgcg aaaaactgca atttggtatt
     4501 atccaatacc ggggttgttt caacagctct catggatttg gaaataaaca cattcttttc
     4561 tgtttctgta agacgcatta aggatgtgtt ttccggcatc catacataca cattcaccat
     4621 gtcaaatttt tgggaatcag ttagccggcc aattaatttt ttttctcgaa aggttctgcc
     4681 gaattcaggg ggaatagcaa agtatttcca tccggaactt gttaatgtgt aattgaaata
     4741 caacatgcga tgcgcctctc gttttcgtaa tacttcaacg tactttgcga gttctttatc
     4801 atagtccttc tgaagcaatt tatacttttt gagcaaagcg ttttctaccg tatccatttc
     4861 tctggtcaac ccttccgatc gcttttggta aaatcttgcc aaagcctttg ccgacgggtt
     4921 tttctcaaca tcgcgcacat ttgttagttt ctctctgcta aatttcaaaa acacttccct
     4981 gtcggctgca ttccctaaca attgagatgc catagagtca accacccata gttgcttatc
     5041 agtattattc acataaaggt ctaatacccg ctgatcaaag gttcggtgaa tggcctgcat
     5101 gcgggtctcg aattcttttg tagcgatgaa tgtattttcg aatgcattct ccttgatcgt
     5161 ttttactttt attgggtcta ttccgcaatc cgaatattca tcgtcagacg atgtgtcggt
     5221 taagcccgat tggtggcttt tttgaatatc cttcagatca aattttttgc cattaggctc
     5281 caataaaaat ttctccccct gatcaggtgt gaatgaagaa agttttttat gtaccagcca
     5341 caccgaataa tacaagctgt cggaaaacga tctgacaaat ttgttttttc cttcaaaggg
     5401 taaaatatca agcaggccgt tatcgaatat ttcaggggtg aaattcaacg tgctcaaagg
     5461 aaccggatca agatattttt ccggcgcaat cggatcgacc caatccatat ttccaactga
     5521 atctctgtta ccgctgtaca tcaacatctc tttagcagtc tttggagtgc taatggtaat
     5581 ggggttgttt tgatcgatat gcagttgttc gttatccttt gtaaaattga tataaaaagc
     5641 actgccaccg ctaagctgcc ccgatgagga gttgcctgtc aaattgctga aaaccaactc
     5701 attaagccca ttggcttgaa ataattccat gttgatcttt ccctgcacca cctcattctt
     5761 ggcgttcaag agtgcaccct tagggattac aattatgagt ccattttctc ccgctaacac
     5821 cgtgtcgatc tctgcagaaa attcaacgaa cttgctaggt gccgatgttt ttgcaaatgg
     5881 atctgttgta gaaaaacgcg tattgacctt agtgtattca gtgtggtcag aacacgagga
     5941 taaagcaaga aacaaaagtg cgatccatat ctgaggtttt ttcatgcgga gtggtatcaa
     6001 caatctaaga tagcattcat ttatgcgatc tgtgcgcaac ttgccaatta tttatttaac
     6061 aagtaggcca tcacactcgc attcctcctt gctaccaaca aatatttctc acgattgagt
     6121 tttgtgagac acatgcttcg cacttctcca tcaatgaaca ggcccgtttc ggcggcgttt
     6181 ttaaatgtga agccgccttt gccatcacca aacaaacagg cgccataacc cgcatccagt
     6241 cttgtcaatt caggtctggt gccaaattga tttcccgcca ggatgaggtc ggggtaatta
     6301 tcgcgattaa tatcttttac tgcgatggca taaaccggaa accattgaat ttgctccggc
     6361 aacaattgtc tggtgaacga gcccttgcca ttgtttataa aaatacttga attgaaatct
     6421 gtagccgtga agtgcgtaat ggtatcccaa tttacatcgg gaaacaaatc gaaaatggaa
     6481 gctttcgcat aatctgcata ataaacatat ctgtttttta gagccggaat ctgttcaagc
     6541 atatcatctt tcgaagctat aggaatttct ttaccacttt tgtcgaacgt tgtgataatg
     6601 ggatcaattt taccatttcg atcaaaatca caaacgtaca tatgtgccgg aaattctttt
     6661 gaggctttta atggatgatt tgttcctctg tttcctgcaa taatgtccat atctccatcc
     6721 aggtcgacat ctacaatatt aatggcattc caccaaccgc tgtaatctga caatccggat
     6781 ggcactgttg attcaataaa tttctttttc ccattgataa aaacgcgtaa tggcgaccag
     6841 tgcgatacca ccactaggtc ttgttttcca tcggcatcaa tatcggccca ctgcgcatcc
     6901 tgtactaatc caatggtatt taattccggg caaatacgcg ctgttatgtc cataaatttt
     6961 ccgccaatat tttcatatat ggctgacgga gaagacaatg gatagcttcc cgctacaaca
     7021 ccgcctccaa gaaaaatatc catgtcgcca tcgttgtcaa agtcacaagt acgcactaca
     7081 gaatgcgcct gtaaactttc gggcaacaat tgattcgcgt tcacatatct gccattcgac
     7141 atatttaaat ataatcggct ctgataatat cgagaacccg ccggatactc atttccgccg
     7201 cttactacaa agaggtctga gtcgccatca ttgtcaacat caatccaggc ggcatcaaca
     7261 tcttcgtatg cagaatcacg ctcccatacg ccaccaaatg acggtatgaa gccttcatca
     7321 cttcccaaaa acaatcgcga ctttttgttt ttgccaccgg tgataaatac atcagaaatg
     7381 ccgtcattat ttgcgtctcc aacggctaaa gccggtccct gatctgaata cacataaggg
     7441 atcaatggtt cttttttaaa gtcatcagca ggttcttcgc ttacactgta aggaatcacc
     7501 ttattgctga catcaataaa ctcaggcaaa taatcagccg cccataattc atattgtcca
     7561 tttttctcat agacaattaa tgttgtgtcg gcagaaatgt ttgacaaaat ttgttccttg
     7621 cctgatgggt atattatttg aattctgtcg acatttttag attcacccaa tccaaagtac
     7681 agcaccggtt ctacggcaga ttgaaagcct ctggtgggca taagttggcg tttgtatgat
     7741 tgattaccta ttgcaacaga aatcatagtg ccaactccaa atgtattttt tccctcgccg
     7801 ataatcttga tttttaaata atgattttgc ttcagctcac gtgcattatt tttaaaaata
     7861 aatacagtat catcaatatt gcttacaaca atatcaagat cgccgtcatt atctaaatct
     7921 gcatatgcag caccgttaga atatgatgga atctgaacac cccaatcaac ggttacattt
     7981 ttaaattgca gcgaatcgct tcctttgaaa caataatttg ataaagggac atcaggcatt
     8041 tgatatacca ggtccaacgt gtttaacttt tcacctgcgt caaaagcttt ttgctcagct
     8101 tcactatagg tatattttaa aaaatccaga ttagtaaaat catgtcgaaa cccatttgtg
     8161 atataaaggt cctgaatacc atccagatcg tagtcggcaa acagcggcgc ccaactccag
     8221 tctgtggctg caacgcctgc gctccaggca atttcagaaa atgaattatt gccattattt
     8281 atttgcaacg tgttaaacat gtgctgataa aaatatccgt aatcggcaga catttgatac
     8341 gcatcaaatt ttgtcggacc ttttaatttt ttttgtcgtt ccggatcttc ggggagcatg
     8401 tccattacca ggagatccaa ccagccatca ttattaatat cggccatatc gcatcccatc
     8461 gaaaaattag aggtgtggcc gaatgcatct tgcagcacct gcgaaaatgt gccgttgtga
     8521 ttgttgatat ataaaaaatc ctgttcgttg taatcgtttg ttacaaatat gtcgaggtat
     8581 gcatcgttat tgacatcgcc gatcattaat cccaatccga aattagatgg cgtgccgaat
     8641 attcccgcct cagcactgat atcggtgaaa taattattgt cgtttctgaa taatttattt
     8701 cctgcatatt tatctcttct gtttcttaca tcgccgatat taaatccatg taattgtgtt
     8761 acggcatgat ttaataaaaa catatccaga tcgccatcat tgtccatatc aaaaaaagca
     8821 gcctgcgtgc tgaacgatga atcgtcgagt ccgaattttt ttgccgattc aacaaatgca
     8881 ccttttccat tattgatata aagttggttg tgtctgtcgc gggtagaaaa ctgtcccgac
     8941 ttacacacat aaatatcgag aaagccatcg ccattcacat ctgccatggt cacccccgaa
     9001 ctccaacccc cgggaggcag aatattagct tgtcgggtag catcgacaaa ttgcatatta
     9061 ccttggttga gatacagttt atcctgccct aaattgccgg taaaaaacaa atcgcaatag
     9121 ccattcccgt ccacatcgcc tgctgcaacg ccaccgccgt tgtagaggta ttgatacagc
     9181 agcacattca tggtagctgt ttcctgtaaa tcgaggtgca tggcaacacc ggtttgggag
     9241 gcggaaagtc ttgagaatag ggtggtttgg gcaaatgtag gtgagtaagc aacaagtgcg
     9301 gaaacaatcc aatatgccac aaattttcta ttcatgcaga aataccctgt tttaggtagt
     9361 caaaattatg tattaattaa ttgtaagtgc ggtttgttga atatcttatt aaactaaagt
     9421 aatagcgctc gaaaacctta caaaaaaatt ctaattgcgg taatgattga gtaaaattgc
     9481 attgattttc gcggtactat caccgccttg atcaaaatga cctatcagta ttatttctta
     9541 aaccaaaaat tttattgttc atgatgcatg tttatgctct catcagacgc gccattttgc
     9601 ccgtggtgtt gttgatgccg gttcttattt ggggacaggc gccaacattg acaggggtga
     9661 tctcggatca gtcaggtgct acgctaccgg gggttacagt gcttattagc ggcaccggta
     9721 aaggcacaat ttccgacttc gacggatctt atgtgcttgc cttcgacgca gcgggaacct
     9781 atactgtaaa ctatacctat atcgggtata aagcagtgac cgaaaccatt acactcaccg
     9841 acgggcaaaa cctgaaaaag gacctcatta tgcaggaaga cgcccttatg ctcgaaacag
     9901 cggttgttgt gggttatggt actatcaaat caaaagacct tacgggttct atcacgaatg
     9961 taggggtgaa agactttcag ggaggtaacg taaccactcc ggagcagctg atcacaggaa
    10021 aggttgccgg tgtgcaaatt acctctaact cgggtatgcc gggttcggga agtcgcatcc
    10081 gcatccgcgg cggtacctcg ctcaatgcaa gtaacgatcc gctcatcgta atcgatggtg
    10141 tgccggttga caataacggt atcagcggct caggaaatgc gctcagcctg atcaacccgg
    10201 atgatattga aaatatcacc gtgctgaaag acgcatctgc agccgccatc tacggttcac
    10261 gcgccgccaa cggtgtaatt atcatcacta ccaaaaaagg attctccgat aataaattgc
    10321 acatcgatgt aagcagcaca tcatcactat cgcaagtgac cggttatgtt gatgtgctta
    10381 ctgcagatca gcttgccgcc gtgctcgata cggcaggtac tgctacacaa caagcgcttt
    10441 taggttctga gaataccgat tggcaaaaag aactttatcg catgggtttt gccgaagacc
    10501 ttgatctcac tttcacaggt ggtatcaaaa atcttcctta tcgcctcaac ctcgaataca
    10561 tgaatgaaag cggtgtgctc gatcgttcgc aactcggacg catgggcgcg tctctcggaa
    10621 ttaatccaac attgctgaac ggaaaattaa aaaccgaagc aaatggtaaa tattatcata
    10681 ccgataactt cttcgccgat cagggtgcaa taggttctgc aattacattc gatccgtcac
    10741 aagctattta tgacgatgct tcggtttacg gcggttattg ggaatggaca aatacatcag
    10801 gtggattaat tccgctcgcg ccaaaaaatc ctgttggatt attaaatcag cgcgatgatg
    10861 aaagtaatgt aaatcgcttt atcggaaaca ttaaactcga ctatgaaata tttaaagatt
    10921 ttcatggagt aataaatggc ggtatcgata tggcgcgttc taacggaaca gttgttgttc
    10981 ctgccgaagc agcttcacaa tatcctaacg gttcaaataa tcaatacgaa caaagcaaag
    11041 acaacaaatt gctcgaagcg tatggtaact ataaaaagga tattaaatcg attaaaagcc
    11101 gcatcgattt tacagcaggt tattcttatc aaaactggct tacgcaatca ccgtcatttc
    11161 caagtttaag tttcgccggc gatactatca ctcctgttgg tatcgacttc gaaaccgaaa
    11221 atacactgat ctctttctat ggtcgtttaa attacaatct gaaagagcgc tacatggtta
    11281 cggctacatt gcgcgaagat ggttcttcac gctttagtcc cgacaaccgc tggggtttat
    11341 tcccatcagt tgcagctgca tggctcgtaa gtgatgaagc gtttatgtcg aatacaaaaa
    11401 tgtatttgaa attacgcgca ggttacggag ttaccggtca gcaggatatt ttctatgatt
    11461 atccgtatat cgcaaactac gatagttcaa cctcaacggc tcaataccaa tttggtgatg
    11521 ctttctatta tttacttcgt cccgatggtt acgatccgaa tatcaagtgg gaagaaacta
    11581 ccagctttaa tattggattt gatttcggct tgaagggaga taaaatttca ggtagtgttg
    11641 atttgtataa aaaactcacc aacgatctcc ttgcaaccat tccgattccg gcaggcacca
    11701 acttcaccaa ccaaattctt actaacgtag gaagcatgga aaataccggt gtcgaaatca
    11761 acctgagtta tatcgctctc gacaacaaag acatgtcgtt agaattcgga attaacggaa
    11821 ccgctaaccg caatgaaatt acaaacctta ctttggtaga agatacttca agcgccggca
    11881 ttttagttgg tggtatagct ggtggtattg gtaatactat tcagattcaa tctgttggtt
    11941 atgcatcaaa tactttcttt gcctatcaac aacaatatga tgaaaatggc aaccctatcg
    12001 aagggtcata tgttgatcag aatggcgata gcatcatcaa tgcagatgat ctgatttggg
    12061 gtgaaaatcc aacacctgac ttttatctcg gttttagcgc tgcattcaaa tacaaaaaat
    12121 ggagtgtatc aactgtgttg cgcggtgaat tcggaaaata catgtataat aacatcaatt
    12181 ctacacgcgg tattttccaa aacgttccaa gtgcaagtta tatgcaaaac ctgtcgacca
    12241 attatctgga aacgggtttc caatcatatc aattgctgag tgattactat attgagaatg
    12301 catcatttgt tcgtatggac aactttgccg ttggatatga cgttggacga gtattcaatg
    12361 acaatgcgtc tctcacagtg ggtgcagtta ttcaaaatgt atttgtaatt tctccatatt
    12421 caggacttga tccggagatt gcaggtggta ttgataattc aatttatcct cgcccacgca
    12481 tttattctct caacattaat cttaaactct aaaccgaaaa gcgatgaaaa ataaaatatt
    12541 aattgcagtc accgcactcg ccatttccgg ttttacgttg agctcatgtt t
//
LOCUS       DAOQOU010000092        12535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2577_length_12535_cov_5.897997, whole genome shotgun sequence.
ACCESSION   DAOQOU010000092 DAOQOU010000000
VERSION     DAOQOU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2577_length_12535_cov_5.897997"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLM90_08965"
     CDS             <1..298
                     /locus_tag="PLM90_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89544.1"
                     /translation="INIAVEKEGITSHEENISLQIYPNPNSGKCYAIINCNEAVNTDL
                     QLLNTVGQVVYQSAANLTPGSNTIYIDARDLPAGVYTMSVKTSTLNISKSIIIE"
     gene            352..1482
                     /locus_tag="PLM90_08970"
     CDS             352..1482
                     /locus_tag="PLM90_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HMZ89545.1"
                     /translation="MKQNRIILGLLFGMSILAVSCSKLNSADSGPQQVSETNTTDTEV
                     VTVDTLHQPADGPPGMVWIPGGTYMMGTDTDPQRRSDETPAHPVKVDGFWMDITEVTN
                     AQFAEFVKATGYLTTAERPIDWEELKKQVPPGTPKPADDLLQPASMVFTSTDHPVDLN
                     NFLNWWKFVPGASWRKPQGDTSTIKGKNNHPVVQVSWDDAVAYCKWAGKRLPTEAEWE
                     FAARGGTTDQIYAWGNDKQLAKHTNSWTGDFPYNNTHEDGYVTTAPVKSYEPNGYGLY
                     DVAGNVWEWVSDYYHSEYYAACKLEGTLVNPQGPKHWYDPNQPFNEVHTKRGGSFLCN
                     EVYCSSYRLTARMATSYDTGQDHSGFRCVMTQEQWEERRNEK"
     gene            1779..4160
                     /locus_tag="PLM90_08975"
     CDS             1779..4160
                     /locus_tag="PLM90_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ89546.1"
                     /translation="MRSITFLLLISISIHAFPQNGKIAGTITNSINNEPVPFATVVLA
                     GTSYGAVSDDLGNYSIEGIEPGLYNIECTFIGFKKVAIPEVQVTNAKPVFINIQMQED
                     LNVLDAIEVKAPVSDKTEESPLSLRTIGANEIQRNPGGGRDISKVIQTLPGVTYTPSF
                     RNDIIIRGGAPNENRFYLDDVEVPNINHFATQGSSGGPVGLINVDFIREVEFYAGAFP
                     VNYGNALSSVMSFQQRDGRSDKWGGTFTVGSSDLGITMEGPIGEKSTVLLSARRSYLQ
                     FLFGAIGLPFLPTYNDFQYKQKLKLDKQNEITIIGLGAIDQFDLNLEDDTSAFQQYVL
                     GNLPVQTQWNYTLGVVYKHYREKGYSTVVASRNMLNNEAYKYENNDDSNPENLIYNYG
                     SQESENKFRYEETANIGRFRIKGGLNYELARYTNSTYQLLPLGTDITTIDYTSKLFVN
                     KYGAFGQASITLLDYRLTLSLGLRLDGNDYSSSMANPLQQLSPRLSASYGLTDKWSLN
                     FNSGIYYQMPAYTVLGYAEGENNFVNKENDLRYIQSSHLVAGLEYRPESYLRFTLEGF
                     YKNYARYPFSLRDSISIANQGADFGVVGDEPVTSTSNGRSYGVEFLAQQRLNKNYYYI
                     ISLTYVVSEFENGDNVYTASSWDNRTIISMTAGKKFKKNWELGLKWRYSGGLPYTPYD
                     QEYSSLTYVWDIYRQGVPDFSKLNAERLDPYHQLDMRVDKKWFFDKWNLDVYVDVQNV
                     YNFQTTFQPYIDVVKDADGNPVINPDDPTRYLTYDLENETGTVLPSIGIIVEW"
     gene            complement(4164..5162)
                     /gene="rfaD"
                     /locus_tag="PLM90_08980"
     CDS             complement(4164..5162)
                     /gene="rfaD"
                     /locus_tag="PLM90_08980"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HMZ89547.1"
                     /translation="MQAGSDNHLKTVVVTGAMGFIGSCLATALHRTGAWRVVISDDFN
                     RPDKMQNLIALQDVLKVERDMIAAWVDSQPAGTISWILHIGARTDTTEFDHAIFDRLN
                     RNATKALWEVCCKQQIPLIYASSAATYGSGVHGYVDNHALIPELQPLNPYGWSKQDID
                     MWVLKQQTHPPFWYGLKFFNVYGPNEYHKGRMASVIFHAFNQMQATGTIKLFRSHKDA
                     YRDGYQLRDFVYVLDVVDVIQWLMHNKPASGIYNLGTGKAEPFYQLAASTAEALNIPL
                     QIDWIDIPEDIRDKYQYFTEADMSKLRKAGYTRAFSSLEAGVADYVKNYLVRGNYY"
     gene            5197..5673
                     /locus_tag="PLM90_08985"
     CDS             5197..5673
                     /locus_tag="PLM90_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89548.1"
                     /translation="MRSLVLFGLVLALCTSCNKRKYAAATEYNDKVIRFVEQSDRAMK
                     VWNSNNFMQEYMIKKHNTVMRLLNMQDSLANTEPLTGDDTLRQAAIMMVENYIESFVV
                     FDTIYAILSDSTYYPEDSIRIHELLSSNRNMLQQQGNNFVETQKRFAIRHDLPFVE"
     gene            complement(5683..6636)
                     /locus_tag="PLM90_08990"
     CDS             complement(5683..6636)
                     /locus_tag="PLM90_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89549.1"
                     /translation="MKNLWVYLGLISLSALGFSSCQQSANGSTNEPSGMQKSVEAFSP
                     GTEWNNYWYSGKAELTSYTLQQSRYGEIHAGTAVNIFVTEDFSKSKHVKLDDPSKAGA
                     DKLPVLKMNQSIKFNTGIYPYSIMLSSFQPVDVNQFPHAVKISGTVQEWCGMAYYQLD
                     QRDEKYQVEVRSYFESEGDKELSLPATVQEDALWNMIRIAPANLPTGEQMILPGATYL
                     RLSHKPVEAVKSQLTLQTIDGVQVYAIKMPSLNRELKIRFESAFPYRILGWEDTYPGF
                     DGVSLTTTAVRNKEMIIDYWRTHNNADRVLREQLGLPKDTQ"
     gene            complement(6736..8964)
                     /gene="purL"
                     /locus_tag="PLM90_08995"
     CDS             complement(6736..8964)
                     /gene="purL"
                     /locus_tag="PLM90_08995"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HMZ89550.1"
                     /translation="MAEALATVEQAKQLGLLPEEFAHITSILGRTPNFTELSIYSVMW
                     SEHCSYKNSIRLLKTLPRSGKMMLVEAGEENAGLIDIGDGLACAFKIESHNHPSAIEP
                     YQGAATGVGGIHRDIFTMGARPIASLNSLRFGNIANARTQHLMKGVVKGIGDYGNCFG
                     VPTVGGEVYFEDCYNTNILVNAMSVGIVKVGETVSAIAEGAGNPVYIVGSATGKDGIH
                     GASFASGDLHEESHEDLPSVQVGDPFQEKLLLEATLEAIKTGAIVGMQDMGAAGITCS
                     TSEMSAKGKHGMKINLDKVPTRQQNMKGWEILLSESQERMLVIVHKGREKEIEAVFDK
                     WDLHCEIIGEVIEGNTLYYYMHGELVAEVPADSLVLGGGAPIYTRETKEPEYLKDIAK
                     YDPNAIAQPADLVAVAKKLAANPNIANKRWITTQYDSMVGVRNTSTNLKSDASLIRII
                     GKNKGIAMTTDCNARYVYSDPYVGTMIAVSEAARNIVCSGAKPAAVTNCLNFGNPYNP
                     EVYWQFSNAIRGMGDACRQFDTPVTGGNVSFYNQSEEGPVYPTPTIGMVGVLEDFKYQ
                     MTMDFINAGNVILLVGNATDDMACSEYLKDIHGITQSPAPRFDLKEEARLQDTIYNLI
                     QKRLIDSAHDVSEGGLFTTLLESAMVNQLGFEIETDHGIRSDAYLFGEGQSRVVVSTT
                     SDKVFEIEAICKSNNIPVNVLGTVTQGSIIIDGVRYGGIQEYREIYDNAIGRYMQQEV
                     TV"
     gene            9175..11085
                     /locus_tag="PLM90_09000"
     CDS             9175..11085
                     /locus_tag="PLM90_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ89551.1"
                     /translation="MKTLFTLPVRVVCCAIFCFMHILAVRAQNMVFNGSFELYDSLPN
                     ETGEWMACTGWNNLNGLGTPDYAHIDGGDVTHMPYTFWCGVYPQDGNAAMSFKTYKDF
                     SENFREYIGTPLIAPLDTTQWYAVSFWITNGDYYHRFGSGTDNIGLLLGTSFRTQPTV
                     HVVDAEPQFVINSMVFNTAWEQHTYYYKPDSAYTCLMMGNFAFDADIATDVFDTAALI
                     FDGAQYFVDNIVIAPIPFVTIIDTGICNGETYILPDGDVAITTLTDTTMLEAIDGSDS
                     LVITHLTVYPTPETNLVVEICNGDSYLLPDGTTTAIAGFYSFNLPSVHGCDSTVNITL
                     TIKDPVEITQNIQRCEGDVYMLPDGNAATTSGIYTTVFTLPSGCDSTIITHLNFATPI
                     IPEMVLPEQICLEAEPIHIETFPEGGIYSAGIGYGNFDPQVFGLGTHTVTYVYTSAAG
                     CTAETTSTITVTSNYANAGPDAVIQQNTTTTLHGNAGGDYTWTPALFLACNTCQETVA
                     MPPVNTMYTLTSVDAVGCIATDSVMISVTDAESDAVWFPNTFTPNGDGMNDYFYPMGY
                     AISDIMSFTIFDRWGERIFESDNQIISGYTGWDGTFNGQAANEGVYVYTANIRLQNNI
                     VTTAKGNVTLVR"
     gene            11488..12426
                     /locus_tag="PLM90_09005"
     CDS             11488..12426
                     /locus_tag="PLM90_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89552.1"
                     /translation="MTEHKMNFSELIERLEKIFRVITAAAIGLSVIEALIVLFIGIAS
                     NNITTVNPDINKVWTGVLISLVVVYAFIIVIKILYNANYPSSITNELKAQHELTELKQ
                     KAERQKTISSFVVATIERLNGATCALNPGDDTHLCDKGVQDGISELIQPVIANTYFLL
                     DTLNTQFTVGLYLADYKTLAQTENDHWDKGIIIINDNLQPSANGIDKSLLQEENLQNE
                     ELEIQTAIRKSLNNHQFVKADFTSMDRELSIICSPMPYACNEDDLLGVLFIICNRLET
                     TPDDLPTTLTIFNRVISNWVYRYNECINSRLELNAQ"
BASE COUNT         3722 a         2729 c         2466 g         3618 t
ORIGIN      
        1 tattaatatc gctgtagaaa aagaaggtat cacttcgcat gaagagaata tttccttaca
       61 aatatatcca aatccaaata gtggaaaatg ttatgccatt attaattgca acgaagctgt
      121 caatacagat ttgcaactgt tgaatactgt tggtcaggtt gtttatcaat cagctgcaaa
      181 tttgacaccg ggttctaaca cgatttatat tgatgcacgt gatttaccgg ccggcgtata
      241 taccatgtcg gtgaaaactt caacattgaa catcagtaaa tcaattatta tcgaataatt
      301 agcttcccct atgaaaccaa aacaaagtaa ttcacgtctt acatttgtag tatgaaacag
      361 aatcggatca tccttggctt gcttttcgga atgagcatcc ttgctgtttc ctgcagtaaa
      421 ctaaattctg cggattcagg gccgcagcaa gtatcggaga caaataccac cgacacagaa
      481 gttgttactg tcgataccct acatcaacct gctgacggac ctccggggat ggtatggata
      541 ccgggcggaa catacatgat gggcactgac actgatccgc agcgccgctc tgatgagaca
      601 cctgcacatc ctgtaaaagt tgatggcttt tggatggata ttaccgaagt gacaaatgca
      661 cagtttgccg aattcgttaa agctaccggt tatcttacaa ctgctgaacg gccaatcgac
      721 tgggaagagt tgaaaaaaca agttcctccc ggaacaccca aacctgctga tgatttattg
      781 caaccagcca gcatggtttt tacctccacc gatcaccctg ttgatctgaa taattttctc
      841 aattggtgga aatttgttcc cggtgcaagc tggcgaaaac cacagggtga tacaagtact
      901 attaaaggaa aaaacaatca tcctgttgtt caggttagtt gggatgatgc agttgcgtac
      961 tgtaaatggg ccggaaaaag attacctacc gaagcggaat gggaatttgc tgcccgcggc
     1021 ggtactaccg atcaaatata tgcctgggga aatgataaac aattggcgaa acataccaat
     1081 tcatggacag gcgactttcc ctacaacaat acgcatgaag atggttatgt aacaacagct
     1141 cctgtaaaat cttacgagcc aaatggttat ggattatatg atgtggctgg aaatgtttgg
     1201 gaatgggtaa gcgattatta tcattcagaa tattatgctg cctgtaaatt agaaggcact
     1261 ttggtaaatc ctcaaggacc aaaacattgg tacgatccca atcaaccttt taatgaagtg
     1321 cacaccaaac gaggtggctc atttttatgt aatgaagttt actgctcaag ctaccgcctt
     1381 accgcacgaa tggctacaag ctacgatacc ggtcaagatc actcaggttt cagatgcgtg
     1441 atgacacaag aacagtggga ggagaggaga aatgagaaat gagaaatgag aaaggagaaa
     1501 ggagaaaggt gagtgggtga gtgggtgagt ttacctcgcc gagtaggcgg gtgggtgagt
     1561 tttggggttg caatgcatgt tttgcggctt tgcaactttg cgtcttagcg cgaagcaaac
     1621 acacaccaca caccacccct ctggtctctg cgcctccgcg tgaaaaaaca caaaccacaa
     1681 aaacacaaac cacaaacacc cacaaacaca atttatattt tcccctatat atccataatt
     1741 ttttaaacaa aaccctctta cctttgttta acgaaattat gcgctccatt accttcttgt
     1801 tgctgatttc tatcagcatc cacgccttcc cccaaaatgg caaaatcgcc ggcacaataa
     1861 ccaatagcat caacaacgaa cctgtacctt tcgcaacagt tgttttagca ggaacttcat
     1921 acggcgctgt ttctgacgac ctagggaatt attccataga aggtatagaa ccaggattat
     1981 ataacatcga atgcactttc atcggtttta aaaaagttgc catccctgaa gttcaagtta
     2041 ccaatgcaaa gcctgtattc atcaacatcc aaatgcagga ggacctcaac gtattggatg
     2101 ccattgaagt gaaagcaccc gttagcgaca aaaccgaaga gtctcccctg tcactccgga
     2161 caattggcgc caatgaaatt caacgaaacc ccggtggcgg tcgcgatatt tcgaaagtga
     2221 tacagacact tccgggtgtt acttatacgc catcttttag aaatgatatc atcatacgcg
     2281 gaggggctcc aaatgaaaac agattttact tagatgatgt tgaagttcca aatatcaatc
     2341 acttcgcaac gcaaggttcc agtggcggac cggtgggatt gataaatgta gattttattc
     2401 gtgaagtaga attttatgcc ggtgcatttc cggtgaatta tggcaatgca ctcagtagtg
     2461 taatgtcatt ccaacaacgc gatggcagat cagataaatg gggaggtaca ttcacagtcg
     2521 gctcgagcga ccttggtatc accatggaag gtccaatcgg ggaaaaatca actgtattgt
     2581 tatcggcacg cagaagttat ttgcaattcc tttttggcgc tatcggattg ccattccttc
     2641 ccacctacaa cgactttcaa tacaagcaaa aattaaaact cgataagcaa aatgaaatca
     2701 ccattatcgg tctcggtgcc attgatcagt tcgatttaaa tctcgaagac gatacaagtg
     2761 cgtttcaaca atatgtattg ggaaatctgc ctgttcagac acaatggaat tatacattag
     2821 gagttgttta caaacattat cgcgaaaaag gatattctac tgttgtcgcc agcagaaata
     2881 tgctgaataa cgaagcttat aaatatgaga ataacgacga ttccaatccg gaaaacctga
     2941 tatataatta tggcagtcag gaatcagaaa ataaatttcg ctacgaggaa acagcgaata
     3001 ttggtcgatt cagaatcaaa ggtggattaa actatgaatt agcaagatat accaattcca
     3061 cctatcaatt attacctctg ggaaccgaca tcacaacaat agattacaca tcgaaattat
     3121 ttgtcaataa gtatggtgct ttcggacaag caagcattac ccttctcgat taccgactta
     3181 ccttatcatt gggacttcgc ctggatggca atgactacag cagttccatg gcaaaccctt
     3241 tacagcagct gtctccgcga ttatcggcaa gttatggact tactgacaaa tggagtttaa
     3301 acttcaacag tggtatttat tatcaaatgc cggcctacac ggtattggga tatgcggaag
     3361 gtgaaaataa ttttgtaaat aaagaaaacg atctccgtta catccagtca tcacatctcg
     3421 ttgcgggatt ggaatacaga ccggaatctt atctgcgatt tactcttgaa ggattttata
     3481 aaaactatgc tcgatatcca ttcagtctcc gcgattctat ttcaattgcc aatcaaggtg
     3541 ccgattttgg tgtagttggc gatgaaccgg taacatccac aagcaatggt cgcagctatg
     3601 gcgtggaatt tcttgcacag cagcgattaa ataaaaatta ctactatatc atatcgctga
     3661 cctatgtggt gagtgaattt gaaaatggcg ataatgtata tactgcctca tcatgggata
     3721 atcgcacaat aatttcaatg acagccggca aaaaatttaa gaaaaactgg gaactcggtc
     3781 ttaaatggcg atactcaggc ggcttgccat atacgcctta tgatcaggaa tactcatcac
     3841 tcacctatgt ttgggatatt tatcgtcaag gcgttcctga tttcagtaaa ttgaatgcag
     3901 agagacttga tccatatcat caattggata tgcgcgttga taaaaaatgg ttcttcgata
     3961 aatggaatct tgatgtgtat gtagatgtgc agaatgtata taatttccaa actacctttc
     4021 aaccgtatat tgatgtagta aaagatgcag acggcaatcc ggttatcaat cctgatgatc
     4081 cgaccagata tctgacttat gatttggaaa atgaaaccgg aactgtatta ccaagtatcg
     4141 gcattattgt agaatggtaa tactcaatag taatttcctc taacgaggta attttttaca
     4201 taatcggcaa cacctgcttc aagactgctg aaagcgcgtg tatacccagc tttgcgcaat
     4261 ttcgacatat cggcttcggt gaaatattgg tatttatcgc gaatatcttc cggaatatct
     4321 atccaatcga tttgcaatgg aatatttaat gcctctgcag tagatgcagc caattgatag
     4381 aatggttctg ctttaccggt gccgaggtta taaattccac ttgcaggttt attatgcatt
     4441 aaccattgaa tgacatcaac aacatccaac acataaacaa aatcgcgtaa ttgatacccg
     4501 tcgcgatagg catccttatg acttctgaat aatttgatgg tacctgttgc ttgcatttga
     4561 ttgaatgcat gaaatattac agatgccatg cggcctttgt gatattcatt cggaccgtac
     4621 acattaaaaa atttcagtcc ataccaaaaa ggagggtgcg tttgctgttt caacacccac
     4681 atatctatat cctgctttga ccacccatat ggatttaaag gttgtaattc cggaattaat
     4741 gcatgattgt caacataacc atgtacgccg gaaccataag ttgccgcaga acttgcataa
     4801 atgagcggta tttgttgttt gcaacaaact tcccacaatg cctttgttgc atttctgttt
     4861 aagcgatcaa aaattgcatg atcaaattcg gtagtatctg ttctggcacc aatatgtaat
     4921 atccaggata tggtgccggc aggctgacta tcaacccacg ctgctatcat atccctttct
     4981 accttcaaaa catcctgcaa tgcaatcaga ttttgcattt tatcaggtct gttaaaatca
     5041 tcgctgatca ccaccctcca tgcgcccgtt cggtgtaatg ccgtcgccag gcaactgccg
     5101 ataaacccca tggcgcctgt taccacaaca gttttgagat gattatcgga accggcttgc
     5161 attttgatta gatttgtaac aaaattacgt caatttatga gaagcctggt gctcttcgga
     5221 ttggttttgg ctctttgcac atcttgcaat aaacgcaaat atgccgctgc tactgaatac
     5281 aacgataagg tgattcgatt tgtcgaacaa agcgatagag caatgaaagt gtggaattca
     5341 aataatttca tgcaagagta catgatcaag aaacataata ccgtaatgcg tttattgaat
     5401 atgcaagatt cattagccaa tacggaacca ttgaccggcg acgatacctt gcgtcaggct
     5461 gctatcatga tggttgaaaa ttatatcgaa tcatttgtgg ttttcgatac tatttatgcc
     5521 attttaagcg atagcactta ttatcctgaa gactccatca gaattcatga acttcttagc
     5581 tccaatagaa acatgttgca gcaacaaggg aataattttg ttgaaacaca aaagcgcttt
     5641 gccatccggc atgaccttcc gtttgtagaa taaactcaac aattattgcg tatccttagg
     5701 caaacctaat tgctctcgca aaacacggtc tgcattgttg tgcgtacgcc aataatcgat
     5761 gatcatttct ttattgcgaa ctgcagtggt agtcaatgaa actccatcaa atccgggata
     5821 agtatcttcc caccctaaaa tgcgatatgg gaatgcagat tcaaatctga tttttaattc
     5881 tctgttcagt gacggcattt taatggcata cacctgcaca ccatcaattg tttgaagtgt
     5941 caattgcgat ttaacggcct ctacaggctt atgtgataaa cgcaaatagg tagcaccggg
     6001 caatatcatt tgttcacctg ttggcaaatt tgctggagca attcttatca tgttccaaag
     6061 tgcatcttcc tgaacagttg ccggcaacga caattcttta tcaccttccg attcaaaata
     6121 acttcttacc tctacctgat atttttcatc gcgctgatct aattgataat atgccatacc
     6181 acaccattcc tgaaccgtgc cggaaatctt aactgcatgt ggaaactgat ttacatcaac
     6241 aggctgaaaa ctgcttaaca ttatagaata tggatatatt cctgtattga attttatcga
     6301 ctgattcatc ttcaacacag gcaatttatc tgcacctgcc ttcgatggat cgtcgagttt
     6361 cacatgcttg ctttttgaaa aatcttcggt aacaaatatg ttcaccgctg taccggcatg
     6421 tatctcgcca tagcgacttt gttgcagggt ataactggtt aactctgctt ttccgctata
     6481 ccagtagtta ttccattccg tgcccggtga aaaggcctca acgctttttt gcatacccga
     6541 aggttcgttg gtggaaccgt tagcagattg ctgacaactg ctgaatccta atgcagataa
     6601 ggatatcagc cctaagtata cccatagatt tttcatgaca ttgaatatta atactgtaaa
     6661 caatcgggca cgccgaaaag tttcatcaaa caaatatatg tatatacatt caatttcgat
     6721 aataccgtaa tggcattaaa cagttacttc ttgctgcata tatcttccta tggcattatc
     6781 gtagatttcg cgatattctt gtatgccacc gtaacgaaca ccatcaataa taatacttcc
     6841 ctgcgttacc gttccaagca cattcacagg aatattattt gatttacata tcgcttcaat
     6901 ttcaaacacc ttatcgctgg ttgttgacac cactaccctg ctttgacctt caccaaaaag
     6961 ataagcgtcg ctgcgaatac cgtgatctgt ttctatttca aatcccagct gattcaccat
     7021 cgcactttcc agcaacgtgg taaataatcc accttcactc acgtcatgtg ccgagtcgat
     7081 tagtcgcttt tgaataagat tgtaaatggt atcctgcaat ctcgcttctt ccttcaaatc
     7141 aaatcgcgga gcaggcgatt gtgtgatgcc atgaatatct tttaaatatt cgctgcatgc
     7201 catatcatca gttgcattac caaccaataa aatcacattt cccgcattga tgaaatccat
     7261 cgtcatttga tatttgaaat cttccaatac accaaccatt ccaatggtag gtgttggata
     7321 cacaggacct tcttcactct gattatagaa cgacacattt ccgcctgtaa ccggggtatc
     7381 aaactgtcga catgcatcac ccataccgcg aatggcattg ctgaactgcc agtatacttc
     7441 aggattatat ggatttccaa aattcaaaca attcgtcacg gctgcaggtt tcgcaccact
     7501 gcaaacaata tttcttgcag cttcactcac tgctatcatc gtaccaacat acggatcgga
     7561 atacacataa cgcgcattac aatctgtagt catggcaatg cctttgtttt ttccgatgat
     7621 gcgaattaaa ctggcatcgc tttttaaatt tgttgaggta ttgcgcacac caaccatgga
     7681 gtcgtattgc gtagtaatcc aacgtttatt tgcgatattc ggatttgcag ctagtttttt
     7741 cgcaacggca actaaatcgg caggttgtgc tatggcattc ggatcatatt tcgcaatatc
     7801 ttttaaatat tccggttctt ttgtttcgcg cgtataaatt ggtgcaccgc ctcccaacac
     7861 caaactgtct gcaggaactt ccgcaaccaa ttcgccatgc atgtagtaat acaatgtatt
     7921 accttctatt acttcaccaa taatctcgca gtgtaaatcc cacttatcga ataccgcttc
     7981 aatttctttc tcacgtccct tgtgcacaat aacgagcatg cgttcctgac tttcggaaag
     8041 taatatctcc caacccttca tattttgctg gcgtgtaggc actttgtcga ggttgatttt
     8101 catgccatgt ttgcctttcg cactcatttc ggatgttgaa caggtgatgc ctgcagcacc
     8161 catatcctgc atgccaacga tagcaccggt ttttatcgct tccaaggttg cttccaacag
     8221 caatttctcc tggaaaggat cgccaacctg cacagaaggc aaatcttcat gactctcctc
     8281 atgtaaatcg ccactggcaa aacttgcgcc atggataccg tcttttcctg tagctgaacc
     8341 aacaatgtaa accggatttc ctgcaccttc ggcaatggca gagactgttt ctccaacttt
     8401 cacaatgccc acactcatgg catttaccag aatattcgta ttgtagcagt cctcaaaata
     8461 cacctcacca ccaaccgtag gcacgccaaa acaattgcca taatcgccaa tgcctttcac
     8521 cacgcccttc atcaaatgtt gggtgcgcgc attggcaata tttccaaaac gcagcgagtt
     8581 cagcgatgct atcggccttg ctcccatggt aaaaatgtct ctgtgaatac caccaacacc
     8641 cgtagcagca ccttgataag gctcgattgc tgatgggtga ttatgacttt cgatcttaaa
     8701 agcgcaggcc agaccgtcgc caatgtctat gagtccggca ttctcttcac cggcttccac
     8761 caacatcatt ttaccgcttc tgggcagtgt tttcagcaat cggatactgt ttttatatga
     8821 acaatgctcg ctccacatga cactgtaaat gctaagttca gtgaaattgg gtgttctgcc
     8881 cagaatggag gtgatatggg caaactcctc gggtaataaa cccagttgtt tagcctgttc
     8941 aacggttgct aatgcttcgg ccatgatggt gaattgaagg tcaaagttac tcgttgaaag
     9001 tcaaatcaga tggcaggctg catgaaatgt ggataaaacg aaaaatatgt ttgcagaact
     9061 cactccctaa ttaatcaggg cacttcacag gaaatatatg gcatgtcgct caatgtgact
     9121 tgacctgcga taagggcatc tctactttta tgccgtattc attaattctc ccgtatgaaa
     9181 acattattta cacttcctgt cagggtagtt tgctgcgcga tcttttgctt tatgcatatt
     9241 ttggcagtcc gggcgcaaaa tatggtattt aacggcagtt ttgaattata tgattcactg
     9301 ccaaatgaaa ccggcgaatg gatggcatgc accggatgga ataacctgaa cggtctcggg
     9361 acacctgatt acgcgcatat cgacggcggg gatgttacac atatgcctta cacattttgg
     9421 tgcggtgtat atccgcaaga tggcaatgcc gccatgagtt ttaaaacata taaagatttc
     9481 tcggagaatt tcagagaata catcggcaca ccacttattg caccactgga tactactcaa
     9541 tggtatgccg tgagtttttg gattaccaac ggcgattatt atcatcgctt cggctccgga
     9601 acagataata tcggattgct gttaggcacc tctttccgca cgcaacctac cgtgcatgtt
     9661 gttgacgcag aaccgcaatt tgtgattaat agtatggtat ttaataccgc atgggagcaa
     9721 catacttatt attacaaacc cgattcagca tatacttgtt tgatgatggg aaattttgct
     9781 tttgatgcag atattgccac cgatgttttc gatactgctg cattgatatt cgacggtgcg
     9841 caatattttg tcgataatat tgtgattgcg cctattccat ttgtcaccat cattgacacc
     9901 ggtatttgta atggggaaac atatattttg cctgacggcg atgtggcaat cactaccctc
     9961 acagatacaa ccatgcttga ggcaatagac ggcagcgatt ctttagtgat tacacatctg
    10021 acagtatacc ctaccccgga aacaaatctt gtcgttgaaa tatgtaatgg cgacagttac
    10081 ttgcttcccg atggcactac aacagctatt gcaggatttt attcatttaa tttaccatcg
    10141 gtgcacggtt gtgatagcac agtaaatatt acattgacaa taaaagatcc ggtagaaata
    10201 acgcaaaata ttcaacgttg cgagggcgat gtttatatgc ttcccgatgg caatgctgct
    10261 accacatcag gcatttatac aacagtgttt accttaccat caggatgtga ttctaccatt
    10321 attacacatt tgaattttgc cactcccatc attcctgaaa tggtgcttcc cgaacaaata
    10381 tgtttggagg cagaacccat tcacatagaa acttttcctg agggaggcat atacagtgca
    10441 ggtattgggt atggcaactt cgatccacag gtatttggat tgggaacaca taccgtaact
    10501 tatgtatata catccgctgc gggatgcaca gctgaaacaa catcaacaat caccgttaca
    10561 tcaaattatg ccaatgcggg acccgacgcc gtgatacagc aaaacaccac aacaacatta
    10621 cacggaaatg ccggtggcga ttatacctgg acgcctgcat tatttttagc atgcaacacc
    10681 tgccaggaaa cagtagccat gccaccggtg aataccatgt ataccctcac cagtgttgat
    10741 gcggttggat gtattgcaac tgatagtgta atgatcagtg taacagatgc ggaatctgat
    10801 gcggtatggt ttccgaatac ctttacacca aacggcgatg gcatgaatga ttatttctac
    10861 cctatgggtt atgccataag tgacattatg tccttcacca tatttgatcg ctggggcgaa
    10921 cgcattttcg aatccgataa ccaaatcatc tccggttaca ccggttggga cggcaccttc
    10981 aacggtcaag ccgcaaacga aggcgtttat gtttacacgg caaacattcg cttacaaaat
    11041 aatattgtca ccactgcgaa ggggaatgtg acattggtga ggtgatggag attttaattg
    11101 cgaatctatt ttttgtttga aatataaatc gtggagagac aaggaattta tccaacgcaa
    11161 tttcattatt gggaactgcc tttaatcttc aaatttcatc ttcgactcct ttaaataaat
    11221 gtgacaggac aaagtggcta tatttcaacc ccgggtgggc tgaaatggct attatatgcc
    11281 atttttaacc tcgtgattgt tgtacttttg tgacatcctg agattaacgg tattccggca
    11341 acgcactatc ctgccgaaag tgtagatttg taagaaataa aataccaatt tgtagcgctt
    11401 tacggcctgt tttggggtgt taggcggtat ggagtagcgg ctataagtaa gttggcggca
    11461 atgctttgac gacagtgcga accttaaatg acagaacata aaatgaattt ttcagaatta
    11521 atagaacgac ttgaaaaaat attccgggta attacggcag cagcaattgg actttctgta
    11581 attgaagctc tcattgttct tttcattggt attgcatcaa acaatattac gacagttaat
    11641 cctgacatca ataaagtttg gactggagtt ttgatttcac ttgtggttgt ttacgctttt
    11701 attattgtca taaaaattct ttacaacgcc aactatcctt catctattac caatgaactt
    11761 aaggcacaac acgaactgac tgaactcaaa caaaaagcag agcgacagaa aacaataagc
    11821 agctttgtgg ttgcaactat tgaacgactt aacggtgcaa cttgtgcttt aaatccaggt
    11881 gacgacacac atctttgcga caaaggagtg caagatggaa taagcgaact tattcaacct
    11941 gttattgcaa acacctactt tcttcttgac actttaaata cacaatttac ggttggactt
    12001 taccttgcag attacaaaac acttgctcaa acagaaaatg accattggga taaaggcata
    12061 atcattatca atgacaatct tcaaccgagt gcaaatggta tagacaaatc tcttcttcaa
    12121 gaggaaaatc ttcagaatga agaattagaa attcagacag cgattagaaa atctctcaac
    12181 aatcatcagt ttgttaaagc ggactttaca agcatggata gagaactttc aatcatttgt
    12241 tcacctatgc cgtatgcttg taacgaggat gatttacttg gtgtgctgtt tatcatctgc
    12301 aatcgtttag aaacaactcc cgatgattta ccgacaacgc ttactatttt taaccgggta
    12361 atttcaaatt gggtttatcg ctacaatgaa tgtatcaata gccgccttga attaaatgca
    12421 caataacctt gaacatataa tctttggaca gaacagtagc acagccgcca acacagggcg
    12481 agcgccataa gcgattttgt gcttcgaaat caatattatc agtaacctaa gtttt
//
LOCUS       DAOQOU010000093        12182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2668_length_12182_cov_6.948792, whole genome shotgun sequence.
ACCESSION   DAOQOU010000093 DAOQOU010000000
VERSION     DAOQOU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2668_length_12182_cov_6.948792"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3575)
                     /locus_tag="PLM90_09010"
     CDS             complement(<1..3575)
                     /locus_tag="PLM90_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HMZ89553.1"
                     /translation="MEGKKWIVAGAIVAVAAMTGLLAAVMTPTKTWAGYDDAWKAVDS
                     LDRISMPESALKLVNEIYDHAKAENISPQIIKSLIYRGKYTNMLEEDGEEKAIALFEK
                     EIAISDGVTKAVLQSLTANLYWQYYSNNRWQISQRTQTEELLSNDIATWDIKALLNKT
                     NELYNASLSQRELLKKTPSTLYVEILLKGNAYGQFLRPTMFDLLGNEALSFYMNSEST
                     LDKPAYAVNIANPILLESAKQFSGYSFNAADSVITQLPAVRILQELTTAHLYDPYPDA
                     LIDLNLSRYLYAKNILSIANVDSLYLQALERDEKQYARDSSSARISLAIATWHMGKRY
                     QYQPLEGDAYKWELRTAETYLNKAIAAFPKSYGGILCKLELNELVRKRLEELNIEQEQ
                     IPGAPILASIKYTNVDKIHLQLLKASANEIVSISSMDYSDRKAALKKLVVERSWTQNL
                     IVDGDLQSHRVEFKIDALGLGSYFLLASANDNWMSDSADVYIRDIWVTNMAYIAESGN
                     GDESDYKRFYVVDRTSGLPMKGVNVTTFRNDYDYSTHKYKESELQKFVTDGNGMFELR
                     DPIIRNTYSFGFTLQNGKDILKTNKLFSIAKSQTQPDREIQHTILFTDRAIYRPGQTV
                     YFKGIMYSETAFGRTKEVLKNHKTTVRFFDVNYQEVGNLELVSNDFGSFSGSFTAPAS
                     GLTGQMQISNETGAVYFSVEEYKRPTFYVELEDLKGDYILNDTISVNGTATAYSGAVI
                     DNAQIQYRVVRSVHMPYWYGDYYFRKSYPVSNADQEIVSGVSATDAEGKFVIPFVAKS
                     QVENVSGLQYTYTVYVDVTDVSGETRSSQTNVTVGKINLQLSADVKDAYHVNSDIAIK
                     ITTHNLNGQPLPATGKVTIFAIDAPKRLMRYRLWDTPDLHLMSEADFIKQFPRDQYAA
                     ELDAGSWKTGAALASDTWNTKENDSVLLPTQGLKQGVYRVVFEALDKSGETVRSEHTI
                     NLLNDGSYAAYPEYFHSVKSENSVEPGNSAIVQVGSSTSGSKALVLIKRYHQPTKAQW
                     VSMMEVAGKKSGVSMMDLEIPVSENDRGGIGVQVMMIKDGREFSKVYNITVPWTNKQL
                     QVKLETFRDKLQPGAKETWKIQVSGEKSDMVSAELLASMYDASLDAFRTNYWGDVYYP
                     ANYLYTSLSAEAMGQSYGWQHNGYFNYYATYPTIEYNQLNWFGFS"
     gene            complement(3656..4219)
                     /locus_tag="PLM90_09015"
     CDS             complement(3656..4219)
                     /locus_tag="PLM90_09015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HMZ89554.1"
                     /translation="MTMHRHLVLAAVVTLPGLGGLGLGLMYFFHPDEIARLTSVSGQE
                     LGFQLLLGTGFAAVSLAALLGVLKLALLKPVVSFFAQLLNQYAISVPVILILSLCAGV
                     GEELLFRGAIQPWLGIWFTAFIFILLHGYLNPLNRPLMFYGTILLVVSAGFGYLCLHT
                     GLYAAMTAHTVIDIALMMHLRKVAKQS"
     gene            complement(4216..5223)
                     /locus_tag="PLM90_09020"
     CDS             complement(4216..5223)
                     /locus_tag="PLM90_09020"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HMZ89555.1"
                     /translation="MIKLRIAVVAGLMIAALFSTSCNKSSSGDKDNQSADTIVAASND
                     TAIAINLESPVADERVLLRFMPEEGKTIYIENNTTFASDESVDTMRIKANSTKYVKAK
                     LVVKGKEKEGYAIDLTLVDVHKTVKDDSGTINYQYNKPMTNPEDEMDRKVEDCMVNST
                     LTLIMTEQGENIDVRGYEAIIKKVKAIVGAQVPDQYIAANIGSPTDNLEYFFISYPDS
                     AVKIGDTWDVEVPSVMQGVPITLKTVFALADRRDGIAYINFNTVASVDKSKLPPEMAA
                     EVDKIHFNAGVKGTGEIFENTGWPRLMKFTQYLDVSDSYQGHATSSKQTGVSTIRIVQ
                     Q"
     gene            5279..8383
                     /locus_tag="PLM90_09025"
     CDS             5279..8383
                     /locus_tag="PLM90_09025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMZ89556.1"
                     /translation="MESTARQDKSFAEAYAQLNDAQRRAVDHIEGPVLVVAGPGTGKT
                     QILAVRIGKILAETDAMPENILCLTYTDAGTVAMRKRLLDFIGADAHRVHIHTFHAFC
                     NDVIQAHLDYFGKRELEPITDLENVMILESILEELPTGHLLKKLKGDLSYEVARLNNL
                     FRMMKEEDWDEKRISDNIDVYLADLPNRTEYTYQRVNNKTGVKVGDPKVSKIAEEQER
                     MEKLRAAAQLFPKYQARMKEAGRYDYSDMILWVVRAFRDPSAVGENMLRNYQERYLYI
                     LVDEFQDTNGAQNEILQLLISFWEVPNVFAVGDDDQSIYEFQGARVKNILDFYTAYQE
                     HVEVIVLTDNYRSHQAVLNAARVVIEQNQERLINKIEGLTKQLTASHPSRQKIDEPKV
                     IEYENPTQEHAGILREVETLLKQGVNPEEIAILYHRHKHADDLITACEKRGIPYQVRK
                     KINILEVPIVQQMLTMLRYLNEEAQSAHSAEHLLFEIMHYPFIGIHPHDVAALSAWIS
                     SRRDPEITWRTVMADPSKLGQIRLRAHESITNLEKNITHWIQETFNLTLQMLFEKVLN
                     ESGLMQYILKNNDRVFLLEAISTLFKHIKAEGMNRPRITIRNYMEVLDQMETHGIQLS
                     IEKTIAQKGGVQFITTHSSKGLEFEYVFLLGCNASNWEKSRGDNRMFAMPDTLTMSGE
                     ENKVESLRRLFYVAMTRAKQHLYISYAAASNEGKVLEASQFVSELLAGENISVEKCNI
                     DAETMMAYQLTSLSPTKEVHIDLFDKDFIASKVEHFVMSASALNTYLDCPLRYYFERI
                     IGVPGAKNDSIAFGNAVHYALRKLFERMRADERQQFPPESVFISDFMHEMERNEDAFT
                     EKQFNNRMELGNTLLSDYYQTHIHQFNKVVVTEYPIRNAEMEGIPITGVFDKIEFIGK
                     DVNVVDYKTGAVKYAREKLLPPSEKNPDGGDYWRQIMFYKILLDSQRTKPWKMISGEV
                     DFIEKDESGEFVRHKFAIASDQVDIVKAQIRDTYARIKNLEFTTGCGDDDCDWCNFVR
                     NLEQ"
     gene            8474..9436
                     /locus_tag="PLM90_09030"
     CDS             8474..9436
                     /locus_tag="PLM90_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMZ89557.1"
                     /translation="MQAAVYTSYGAPDVVTVRDIPTPRPGEKDVLIRVHASTVNRTDC
                     GFRSAEYFISRFWSGLFKPKRQVLGCEFAGVVDSVGSSVTMFKPGDKVFGFNDRTFGG
                     HAEYICQPENSAMDIIPEHLNFEQAAALTEGSHYALNDIYAAHVKAGDHVLVYGATGA
                     IGSAAVQILKYLNAHVTAVCGTAHVDLIKSLGADVVIDYMQQDYTQQGLTFDLVFDAV
                     GKTSFGTCKPVLNEPGIYISTELGKNGQNVWYSIFNAFTGNKKVLFPLPVYDKEMISF
                     IRKIADDKHFTPVLDKTYALKDIREAYAYVETGTKVGNVIIRCI"
     gene            9491..10108
                     /locus_tag="PLM90_09035"
     CDS             9491..10108
                     /locus_tag="PLM90_09035"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HMZ89558.1"
                     /translation="MANNYSAFTAHPWHGIHIGDKAPEVVNAYIEIVPTDTVKYEIDK
                     PSGFLKIDRPQKYSNVLPCLYGFVPKTYCGPGIGNFCSQQTGRAGIVGDGDPLDICVF
                     TERNIPHGNIIVPAKLIGGFRMIDGGQADDKIIAVLPFDEIYSMWEYIQQMPSSLVER
                     LRHYFLTYKALPGAPNKAEITHLYGREEAIRILELARKDYAELIK"
     gene            10271..12076
                     /locus_tag="PLM90_09040"
     CDS             10271..12076
                     /locus_tag="PLM90_09040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ89559.1"
                     /translation="MGEKASLYAPLLVETAWEVCNQVGGIYTVVRSKAPAIVDKWSGN
                     YCMIGPLLNAKLPAEFEEVFSYDDVFVEAVTAMRNKGYEVHYGRWLTDGKPKVILMSP
                     MQVYDKMKDIRTDLLRDHKINIFNPDDLLQQVIAFGYVVAQFFSTLEATAKGRPVIAH
                     FHEWMTGMPIGHIRKEGLKVKTVFTTHATLLGRYIAGNDEQFYRHLSNYDWLQMAQKF
                     YIEPQTRLERMAAADCDVFTTVSDITGKECEFLLGRKPDMILPNGISLTRFLATHELQ
                     NLHGQFRDKIDEFVMSHFFHSYTFDLNQTIYFFTSGRYEYHNKGYDLTLEALRRLNER
                     MKKENAQKTVVMFFITKRPFETINPLVLHARAAMDDIRSTCEEMLKQVKDKLFHAAAT
                     NVDPNVPDLNEFIDDYWKFRYRKQIQGWKSDFWPIVVTHNLKDDGGDEILNYLRSNDL
                     INKPTDKVKMVYHPDFVAPTSPLFGMDYPQFVRGCHLGLFPSYYEPWGYTPLECVARG
                     VPAVTSDLSGFGNYCQSKFRNLDERGIYIVRRKTKTFDESADELCDIMFNYTQLARRD
                     RIILRNTVESYAEHFDWSNLAANYEKAYQMVLQQE"
BASE COUNT         3529 a         2509 c         2616 g         3528 t
ORIGIN      
        1 aaagaaaatc caaaccaatt caattgattg tattctattg taggataagt ggcgtaataa
       61 ttgaaatatc cattatgctg ccatccgtat gattgtccca tcgcctcagc ggaaagcgag
      121 gtatacaaat aattagcagg ataataaaca tcgccccaat aatttgttct gaaggcatcc
      181 aatgatgcgt cgtacataga tgccaataat tcggcactca ccatgtcgct cttttcacca
      241 cttacttgta ttttccatgt ttccttagca cccggttgca atttatctct gaaggtttca
      301 agtttcactt gtaactgttt gttcgtccaa ggaaccgtaa tattatatac tttagaaaat
      361 tcacgtccat cttttatcat cattacctga acgccaatgc ctcctctgtc attttccgaa
      421 accggaattt caagatccat catggatacg ccggattttt ttccggcaac ttccatcatg
      481 gaaacccatt gtgcttttgt aggttgatga tatcttttga tcagcaccaa agctttagat
      541 cctgatgttg agcttcccac ttgcacaata gcgctatttc cgggctctac ggagttttcg
      601 cttttcactg aatgaaaata ctcggggtaa gcggcataag agccatcatt caacaaattg
      661 atagtgtgtt cgctgcgcac tgtttcaccg gacttgtcga gcgcttcaaa aactacgcga
      721 taaacgcctt gtttaaggcc ttgtgttgga agtaataccg aatcattctc ctttgtattc
      781 catgtgtcag atgccaaagc tgctcctgtt ttccaacttc ctgcatccaa ctcggcggca
      841 tactgatcgc gaggaaattg tttaataaag tcggcttcac tcatcaaatg caaatccggt
      901 gtatcccaaa ggcgatatcg catcaaacgc tttggtgcgt caatggcaaa tattgttact
      961 tttccggtag caggaagcgg ctgtccattc aagttatggg tagttatttt tattgctatg
     1021 tcactgttaa catgatatgc atcttttaca tctgcactca actgcagatt tatttttccc
     1081 acagtcacat ttgtttgact actgcgcgtt tctccggaaa catcggtcac atcaacataa
     1141 acagtatagg tatattgcaa tcccgaaacg ttttctacct gcgatttagc tacaaacggt
     1201 attacaaatt ttccttcagc atctgttgca cttacacctg aaacaatttc ctgatcagca
     1261 ttacttacag gatatgattt cctgaaataa taatcgccat accaatatgg catatgaaca
     1321 cttcttacca cgcgatactg tatttgcgca ttatcaatta ctgcgccgct atacgccgtt
     1381 gctgttccgt ttacggaaat ggtgtcatta agtatataat ctcctttcag gtcttctaat
     1441 tcaacataaa atgtcgggcg cttatattcc tccacagaaa aatacaccgc tccggtttca
     1501 tttgaaattt gcatttgtcc ggtcaatccg ctcgcaggcg ctgtaaagct tcccgaaaat
     1561 gaaccaaaat catttgacac caattcaagg ttccctactt cctgataatt tacatcaaaa
     1621 aaacgaacag tggttttatg attcttaagt acttcttttg tacggccaaa tgctgtttcg
     1681 ctgtacatga tacctttaaa ataaaccgtc tgtcccggac gataaattgc cctgtcggta
     1741 aacaaaatgg tatgttgaat ttctcgatca ggctgtgttt gcgatttcgc aatgctaaac
     1801 agcttatttg ttttcaatat gtcttttcca ttctgcaaag tgaatccaaa tgaataagtg
     1861 ttcctgatta tcggatcacg caattcaaac atcccattgc catcggtcac aaatttctgt
     1921 aactcactct ctttatattt atgtgtgcta taatcatagt cgttgcggaa cgtggtaaca
     1981 ttaacacctt tcatcggcaa tccgcttgtt ctgtctacaa cataaaatcg cttgtaatcg
     2041 ctttcatcac cattgccgga ttcggcgatg tatgccatat ttgttaccca tatatcacgg
     2101 atatacacat ctgcagaatc gctcatccag ttatcattgg cagaggcaag cagaaaatat
     2161 gaaccaagac caagtgcgtc aattttaaat tctactctat ggctttgaag gtcgccatca
     2221 acaattaaat tttgcgtcca tgaacgttct acaacaagtt tcttcagcgc cgcttttctg
     2281 tcgctataat ccattgagga tatcgataca atctcgttag cagacgcttt gagtaattgc
     2341 agatgaatct tatctacatt ggtatactta attgatgcca atataggtgc tccgggaatt
     2401 tgttcctgct cgatatttaa ttcttccaat cgcttgcgaa caagctcatt taattccagt
     2461 ttacataata tgccgccgta acttttagga aatgcagcaa ttgccttatt caagtacgtt
     2521 tcagctgtgc gcaattccca tttataagca tcgccttcca aaggttggta ctgatatctt
     2581 ttccccatat gccaggtagc aatggcaagc gaaattcttg ctgaggagct atcgcgggca
     2641 tactgttttt cgtcgcgctc aagtgcttgc aaatataaac tgtcgacatt agcaatcgac
     2701 agtatgtttt tagcatacaa atatctgctc agattaagat cgatcaatgc atccggatat
     2761 gggtcgtata aatgtgctgt tgtaagctct tgcaaaatgc gcaccgcggg caattgtgtg
     2821 ataacactgt cagctgcgtt gaaactatat ccagaaaatt gtttcgcaga ttccagcaaa
     2881 attgggttgg cgatattcac cgcataggct ggcttgtcta atgtgctttc gctattcata
     2941 taaaaactca acgcctcatt acccagcagg tcgaacatgg tggggcgtaa aaactgacca
     3001 tatgcatttc ctttcaaaag aatttcaaca tataaggttg aaggtgtttt ttttaataat
     3061 tcgcgctgcg acaatgatgc attgtacagc tcatttgttt tgttgagcag cgctttaata
     3121 tcccaagtag caatatcgtt gctcaataac tcttctgttt gtgtgcgttg tgaaatttgc
     3181 cagcggttat tagaataata ttgccagtac aaatttgctg tcaggctttg caataccgct
     3241 tttgttacgc catcagaaat ggctatttct ttttcaaaca atgcaattgc tttttcctcg
     3301 ccatcttctt ccagcatgtt ggtgtatttc ccacgataaa tcaaagactt aattatctga
     3361 ggactaatat tttcggcttt ggcgtgatca taaatttcat tcaccaattt caatgcactc
     3421 tcaggcatgc taatccggtc caacgagtca accgctttcc aggcatcgtc atatcccgcc
     3481 catgtttttg ttggtgtcat taccgctgca agtaatcctg tcatagccgc aaccgctaca
     3541 attgctccgg ccactatcca ttttttccct tccataatta tttctcttat tctataaatg
     3601 taataaagtc aagtcttatg taaccataag atgcatcgcc tttccaattt tcacatcagc
     3661 tttgtttggc aacctttcgc aaatgcatca tcaatgcaat atcaatcacg gtgtgtgctg
     3721 tcatagcggc atagagtccc gtatgcaaac acaggtatcc gaatccggca cttaccacca
     3781 acaatatcgt gccataaaac attagcgggc ggtttagcgg gttgaggtag ccatgaagca
     3841 ggatgaaaat aaaggcagtg aaccaaattc ccagccaggg ctgaatggcg cctctgaata
     3901 acaactcttc cccgacgccg gcacataacg acaaaatcag gatcaccggt actgagatag
     3961 cgtattgatt gagcaattgg gcaaaaaacg atacaaccgg ttttagcaaa gccagtttaa
     4021 gcacccccag gagggctgcc agtgaaacag ccgcaaatcc cgtgcccaaa agcaactgaa
     4081 accccaattc ttgccctgaa acgctagtga gacgggcaat ttcgtcggga tgaaaaaagt
     4141 acatcaaccc caaacccaag ccacctaaac cgggcaacgt gacaaccgcc gccaggacca
     4201 gatgtcggtg catggtcatt gctgcacgat gcggattgta gaaacgcctg tttgtttgga
     4261 actggtagca tgaccctggt aggagtcgct cacgtcgagg tactgggtaa atttcatcaa
     4321 ccttggccag ccggtatttt cgaagatctc gcccgttcct ttgactccgg cgttgaagtg
     4381 tatcttatcc acctcagcag ccatttcagg cggcagtttc gatttgtcga cgctggcaac
     4441 cgtgttgaaa ttgatatatg ctatcccgtc gcgacggtcg gctagggcga aaacggtttt
     4501 tagtgttatg ggcacaccct gcattactga tgggacttcc acatcccagg tatccccgat
     4561 tttcacggcg ctgtcgggat aggaaatgaa aaaatattca aggttgtcgg ttggtgagcc
     4621 gatgtttgct gcgatatatt gatcgggcac ctgtgcacca actattgctt ttaccttttt
     4681 aatgatagcc tcataaccac gcacatcgat gttttcaccc tgctctgtca ttatgagggt
     4741 gagggtgcta ttcaccatac agtcttcaac ctttctatcc atttcgtctt ccggattcgt
     4801 catcggtttg ttgtactgat aattaatggt gcccgaatcg tcttttaccg ttttgtgcac
     4861 atcaaccagc gtgagatcaa tagcatagcc ctctttttct ttgcccttta caacgagctt
     4921 tgctttcaca tacttagttg aatttgcctt aattcgcatg gtatcaaccg attcgtcaga
     4981 agcaaatgtt gtgttgtttt cgatataaat cgtctttcct tcttcaggca taaagcgaag
     5041 taaaacacgc tcatctgcta cagggctttc gaggttgatt gcaatggcgg tatcattgct
     5101 tgcagcgaca atggtgtcgg cgctttgatt atctttatcg cctgatgacg atttgttgca
     5161 ggaagttgaa aatagtgcag cgatcatcag accggcaacc acagcgattc ttaattttat
     5221 catttgcaat gatgcgttga gttgatgtga aattcgcctg caaagctaca tgaataccat
     5281 ggaaagtact gcccgacaag acaaatcctt cgccgaagcc tatgcacagc ttaacgacgc
     5341 acagcgcaga gctgttgatc atatcgaagg tccggtgttg gtggtagccg gcccgggaac
     5401 aggtaaaaca cagatccttg ctgttcgaat aggaaaaatt ctggcagaaa cagatgcgat
     5461 gccggagaat attctctgtc ttacatacac cgatgcgggc actgttgcca tgcgcaaacg
     5521 tttgctcgat ttcattggtg ccgatgcaca tcgcgtacac attcatacat ttcacgcctt
     5581 ttgcaatgat gtaatacaag cgcatctcga ttatttcgga aagcgcgaat tagagcccat
     5641 taccgatctg gaaaatgtga tgatattgga atcaatttta gaagaacttc ctacgggcca
     5701 tctgcttaaa aaactgaagg gcgatcttag ctatgaagta gcgcgattga acaacctttt
     5761 tcgcatgatg aaggaggagg attgggatga aaagcgcatt agtgataata tcgatgtata
     5821 tcttgccgat cttccgaata gaacggaata cacttaccaa cgtgtaaaca acaaaaccgg
     5881 cgttaaagtt ggtgatccga aagtaagtaa gattgcagaa gaacaggaac gcatggaaaa
     5941 actgagagcc gctgcacagt tgtttccaaa atatcaggca cgcatgaaag aagccggtcg
     6001 ctacgattac agcgacatga tcttatgggt agtgcgtgca tttcgcgacc catccgccgt
     6061 gggcgaaaac atgttgcgca attatcagga gcgttaccta tatattttag ttgatgaatt
     6121 tcaagatacc aacggcgcac aaaatgaaat ccttcaatta ttaattagtt tctgggaggt
     6181 gccgaatgta tttgccgttg gcgacgacga tcaaagtatt tatgaatttc aaggtgcgcg
     6241 tgtaaaaaat attctcgatt tctataccgc atatcaggag catgttgaag tgatcgtgct
     6301 cacagataat taccgctcgc atcaggcggt attaaatgct gcgcgagtgg tgattgaaca
     6361 aaatcaagag cggcttatca ataagatcga gggcttgacc aaacagttaa cggcatcaca
     6421 cccgtcgcga caaaaaattg atgagcctaa agttattgaa tacgaaaacc ccactcaaga
     6481 gcatgccgga attttacgtg aagtagaaac gctgctaaag cagggagtaa atccggaaga
     6541 aatagccata ttatatcaca gacacaaaca tgctgatgat ctgataactg cttgcgaaaa
     6601 acggggaatt ccatatcagg taagaaagaa aataaatatt cttgaagtgc ccattgtgca
     6661 gcagatgctc acgatgttgc gatatttaaa tgaagaggcg caatcggcac acagcgcaga
     6721 gcatctgttg ttcgagatca tgcactatcc atttattggc atacatccac atgatgtagc
     6781 ggcattatcg gcatggatat cttcacgtcg cgacccggaa attacctggc gcacggtaat
     6841 ggctgaccca agtaagttgg gacaaatacg attgcgcgca catgagtcaa taacaaatct
     6901 ggaaaaaaat atcacccact ggatacagga aacatttaat ctgacactgc aaatgttgtt
     6961 tgaaaaggtg ttgaatgaaa gcgggttgat gcagtatatt ctcaagaaca acgacagagt
     7021 gtttctatta gaggcaatca gcactttatt caagcatatt aaagctgaag gaatgaatcg
     7081 cccgcgcatt accattcgca attatatgga ggtgcttgat caaatggaga cacatggtat
     7141 tcaattgtcg attgagaaaa ctattgcaca aaaaggcgga gtgcaattca ttacaacaca
     7201 tagcagcaag ggcttggaat ttgaatatgt gtttttattg ggatgcaatg catctaattg
     7261 ggagaagtcg cgcggagata atcgcatgtt tgccatgccg gacaccttga ccatgagcgg
     7321 agaggaaaac aaagtagaat cgcttcgacg cttgttctat gttgctatga caagagcaaa
     7381 acaacatctg tatatttcat atgcagctgc aagtaatgaa ggaaaagtat tggaggcctc
     7441 gcaatttgtt tctgaattat tggcaggtga aaacatttcc gttgaaaaat gtaatatcga
     7501 tgcagaaaca atgatggcat atcaactaac atcattatca ccaacgaaag aagtgcatat
     7561 tgatttattt gataaagatt ttattgcttc aaaggttgag cattttgtga tgagtgcgag
     7621 tgcattgaat acttacctag attgtccttt gcgctattat tttgaacgta ttataggcgt
     7681 tcccggagca aagaacgata gcatagcctt tggtaatgcc gtgcattatg ctttgcgcaa
     7741 gctgtttgaa cggatgaggg ccgatgagcg tcagcaattt cctccggaat cggtgtttat
     7801 cagcgatttc atgcacgaga tggagcggaa tgaagatgcg tttaccgaaa aacaatttaa
     7861 taatcgcatg gaattgggta atacgctttt gagcgattat tatcagacgc atattcatca
     7921 gttcaataaa gttgtggtta cggaataccc cattcgcaat gcggaaatgg agggcattcc
     7981 gatcaccggc gtatttgata aaattgagtt tataggaaaa gatgtcaatg tagttgatta
     8041 taaaaccggc gctgtaaaat atgcgcgtga aaaattactt ccaccaagcg aaaaaaatcc
     8101 ggatggcggc gattattggc gacaaatcat gttttataaa atattgttgg acagtcagcg
     8161 cacaaaaccc tggaagatga tcagcggcga ggttgacttt attgaaaaag acgagagcgg
     8221 tgaatttgtg cgacataaat ttgcaattgc ctcagaccaa gttgacatag ttaaagcaca
     8281 gatccgcgac acgtatgcgc gcataaaaaa cctagaattc acaacaggct gtggcgacga
     8341 cgattgcgat tggtgtaatt ttgtaagaaa tcttgagcaa taactttctt cttaggcata
     8401 atttattatt gattattcgg tgtaaaataa tcagtatatt tatttcataa atatttcttt
     8461 aaaacatttt gcaatgcagg ctgcggttta tacttcttat ggggctccgg atgttgtaac
     8521 ggttcgtgac atccccacac cacgacccgg tgaaaaagat gtattaatcc gtgtgcatgc
     8581 atctaccgta aacaggacag attgtggatt cagaagtgcc gaatatttta tttccagatt
     8641 ttggagcggc ttatttaaac caaaacgcca ggtacttggg tgtgaatttg cgggcgtggt
     8701 agattctgtt ggcagcagtg tgactatgtt taaacccggc gacaaagtat tcggattcaa
     8761 cgatcgcaca tttggcgggc atgctgaata tatttgtcag cccgaaaaca gtgcaatgga
     8821 tattattccc gagcatttaa attttgagca ggcggccgca cttacagaag gctcacatta
     8881 tgcattaaat gatatttatg cggcacatgt taaagccggc gatcatgttt tagtttatgg
     8941 tgcaacaggc gcaatcggat ccgcagcggt gcagatacta aaatatttga atgcacatgt
     9001 taccgcagtt tgtggaacgg cacatgtaga tctgattaaa tctttaggcg ctgatgttgt
     9061 tatcgattat atgcaacagg attacacgca acaaggattg acttttgatt tggtttttga
     9121 tgccgtggga aaaacatcat ttggcacatg taaacctgta ttaaacgagc cgggaattta
     9181 tatcagcact gaattgggaa aaaacggtca gaatgtttgg tattccattt tcaatgcatt
     9241 tacgggtaat aaaaaagtgc tttttccgct tcccgtttac gacaaagaaa tgataagttt
     9301 tattcgcaaa atagccgatg acaaacattt tacacctgtt ttggataaga cttatgcgtt
     9361 gaaagatatt cgtgaagcat acgcgtatgt tgaaaccgga accaaggttg gcaacgtgat
     9421 tatccgctgc atataatatc gctttgcaag catgcattgt gtacatttgt gtgattaata
     9481 tttcattttc atggcaaaca attattcagc atttacagca cacccctggc atggtattca
     9541 cattggcgac aaagcgcctg aggtggtgaa tgcatacatt gaaattgttc caaccgatac
     9601 ggtgaagtat gaaattgaca agccaagtgg ttttttgaaa atcgacaggc cccaaaaata
     9661 ttcgaatgtg ttgccttgtt tgtatggctt tgttccaaaa acatattgcg gccccggtat
     9721 cggaaatttt tgttcacaac aaaccggaag agcgggaatt gtgggcgacg gcgacccctt
     9781 ggatatctgt gtttttactg aacgcaatat tccgcatggc aacataatcg tgccggctaa
     9841 gctcatcggt ggtttcagga tgatcgatgg cggtcaggct gatgacaaga tcattgccgt
     9901 tttgcctttt gacgagattt actccatgtg ggaatacatt caacaaatgc caagctcttt
     9961 ggttgaaagg ctgagacact atttccttac ctacaaggcc cttcctggag ctccgaataa
    10021 ggcggagatt acccatttat acggtcggga agaagctata aggattcttg agcttgcccg
    10081 aaaggattat gccgaattga taaaatagtg cttacgccat aggcatattt ttagcaccac
    10141 ttctatgcac ttaagcagct ctcgactgcg cgttttttct tttttatgcc gatggtgacc
    10201 ggtatttcct tttgtaatcg aagggttgtg taatttcgtg acgttgagtt aaccataatc
    10261 agataatatc atgggtgaga aagcgagttt atatgcgccg ctattggtcg aaacggcttg
    10321 ggaagtatgt aatcaggtag gtggaattta tactgttgtg cggagtaagg ctcctgcaat
    10381 agtagacaag tggtcgggaa actattgtat gataggacca ctgcttaatg ccaagcttcc
    10441 tgccgaattt gaagaggtat tctcatacga cgatgttttt gtcgaagcgg taacggcaat
    10501 gcgtaacaaa ggatatgaag tacattatgg tcgctggcta acagatggaa aaccgaaagt
    10561 gatattgatg tcgcccatgc aagtgtacga caaaatgaaa gacattcgca ccgatttatt
    10621 gcgcgaccac aaaatcaaca tttttaatcc ggatgatttg ttacaacagg tcatcgcctt
    10681 cgggtatgtg gttgctcaat tcttcagcac attagaagca acggcaaaag gtcgccccgt
    10741 aattgcgcat tttcatgagt ggatgaccgg tatgccgata ggacatattc gcaaagaagg
    10801 gttaaaagta aaaacggtat ttacaacaca tgcaacttta ttgggaagat atattgcggg
    10861 taatgatgaa caattttatc ggcatttgag caattacgac tggttgcaga tggcacaaaa
    10921 attctatatc gaaccacaaa cgcgattaga aagaatggct gccgcagatt gtgatgtgtt
    10981 tactacagta agcgatatca ccggaaaaga atgtgaattt ttattgggac gaaaaccgga
    11041 tatgatttta ccgaatggta tcagcttaac acgatttctt gcaacacatg aattacaaaa
    11101 tttgcatggt caattccgag ataagattga tgaatttgtg atgtcgcact ttttccattc
    11161 ttacaccttc gatctcaatc aaacgatata tttcttcacc tcgggaagat atgaatatca
    11221 caacaaagga tatgatttaa cattagaagc gctgcgcaga ttaaatgagc gcatgaaaaa
    11281 agaaaatgcg cagaaaacgg ttgtgatgtt ttttattacc aaacgtccct tcgaaacaat
    11341 taacccgctt gtgctgcacg cccgcgccgc aatggacgat atccgcagca cttgtgaaga
    11401 gatgttgaaa caagtgaaag ataaattatt tcacgccgcc gctaccaatg tagatccaaa
    11461 cgttccggat ctgaatgaat ttatcgatga ttattggaaa ttcagatatc gaaaacaaat
    11521 tcaaggatgg aagagcgatt tctggcctat tgttgtaaca cataatttaa aggacgatgg
    11581 tggtgatgag atattaaatt acttgcgtag caatgacctc atcaacaaac caaccgacaa
    11641 agtgaaaatg gtttatcatc cggattttgt tgctcctaca tcgccgttat ttggaatgga
    11701 ttatccgcaa tttgttcgcg gttgtcattt gggattattt ccaagctatt atgaaccatg
    11761 ggggtataca ccattggaat gtgttgcgcg tggggttccg gctgttacca gcgatttaag
    11821 tggatttgga aattactgtc aatcaaaatt ccgcaacctc gatgaacgcg ggatctatat
    11881 cgtacgcaga aaaacaaaaa cctttgatga aagtgctgat gagttgtgcg atatcatgtt
    11941 taactataca caactcgccc gtcgcgacag aattatttta agaaacacgg tagaaagtta
    12001 tgccgaacat ttcgactgga gtaatctcgc agctaattac gagaaggcat accagatggt
    12061 gttgcaacag gagtgaggga atgtgagaat tagcaagttt gagaattagc aaattagcaa
    12121 gtgtggagtg cttttaacgc tggcgcgtta atagggaatg caatgatatt gctttgcaat
    12181 at
//
LOCUS       DAOQOU010000094        11959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2740_length_11959_cov_7.363911, whole genome shotgun sequence.
ACCESSION   DAOQOU010000094 DAOQOU010000000
VERSION     DAOQOU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2740_length_11959_cov_7.363911"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..773)
                     /locus_tag="PLM90_09045"
     CDS             complement(141..773)
                     /locus_tag="PLM90_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HMZ89560.1"
                     /translation="MANLLNTLTNSIKHWYVPLIVGILFVVFGIYIFTVPLETYVTLS
                     VIFSISFITSGLFEIFFSIQNAKTLSGWGWYLVGGLFSLAMGIYLVMYPGISITILPF
                     VVGFTVMFRSFQLLGIAFDMKGMGILRWGNLAIASTLGIIFSFILLANPIFSGISLVT
                     ATALAFIFVGVSLAILSFDLKKVKDFPSKISNNLKEKIASLQNELKGTSQ"
     gene            complement(791..1210)
                     /locus_tag="PLM90_09050"
     CDS             complement(791..1210)
                     /locus_tag="PLM90_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89561.1"
                     /translation="MAQKLSELLAELTAKAKQLEDKFEASKGQTKEQLQQWQNEAKEN
                     VAETKAAFEAKKASLEGEAKSHWDNVKSNWDNGVAKLKSDFRKAKYNLEASDAELSAE
                     WAEDDAEMSVYFALDAISDAQEAVIDAVNARAKANSY"
     gene            complement(1314..1664)
                     /locus_tag="PLM90_09055"
     CDS             complement(1314..1664)
                     /locus_tag="PLM90_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89562.1"
                     /translation="MKLKNLLGLATGIILMCQSDLVNAQLYTYTNDETGVAGTVATGV
                     TASNLTPVNGVVAINACGTGYSSDTWSTSTGAFSTAFSAIQLTLTPTTGYNVTVTAIQ
                     FDLGRNPQGPQRMR"
     gene            1914..2816
                     /gene="gldA"
                     /locus_tag="PLM90_09060"
     CDS             1914..2816
                     /gene="gldA"
                     /locus_tag="PLM90_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HMZ89563.1"
                     /translation="MSVKVQNITKIYGEQRAVDDISFTVSPGEILGFLGPNGAGKSTT
                     MKILTCFIPQTGGEASVCGFDINKQAVEVRRQIGYLPEHNPLYKEMYVQEYLEFAANI
                     NGMKKASARINEMIEITGLVPERKKKIGQLSKGYRQRVGLAQAMLHDPKVLILDEPTS
                     GLDPNQLVEVRNLIKNIGKDKTVILSTHIMQEVTAICNRVIIINKGKLVADDSISSLQ
                     SRQKGITVVLAEFKETVDKGMLMRINGVSKVAAEGKALRIEYTGREDMRELLFKFAVE
                     HKLTLLNLQIETKSLEDIFHTLTK"
     gene            2858..3589
                     /gene="gldF"
                     /locus_tag="PLM90_09065"
     CDS             2858..3589
                     /gene="gldF"
                     /locus_tag="PLM90_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HMZ89564.1"
                     /translation="MFAIFKKELNVFFSSLIGYIAITIFLVANGLFTWVFPETNILDE
                     GFATLDAFFNFAPWILMFLIPAITMRSFAEEKSSGTIEFITTRPVTDIQIILGKYLGA
                     FVLVIFSIIPTITYYFAIRGLASPVGNVDTGGIIGSYIGLILLGACFVAIALFTSAVT
                     NNQIVAFISGMFACFFFYLAFDSLSLIPVFVGSADSYFNALSINQHYQNISKGYIDLR
                     DVVYFFSMITIFILMTKTALGSRKW"
     gene            3600..5336
                     /gene="gldG"
                     /locus_tag="PLM90_09070"
     CDS             3600..5336
                     /gene="gldG"
                     /locus_tag="PLM90_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HMZ89565.1"
                     /translation="MDAKLLYKKNRRRELLQLLLLTAIIVIVNVVVSGVFFRIDLTQE
                     KRYSLSTPTKELLRAQDDIILARVYLAGNLTPNLQRLQQSTVEMLDQFKNYAGENIQY
                     DIFDPFSIPDETNQTELIKELEDKGILSVQFFESATDEASVKYVFPFISLTYRDRELV
                     FPLIDRGTMPLPLNPDSDPSVSISLLEYKFTKAIRQLTTEEKPYIAFISGHGELNRLE
                     LNDMAASLNELYNVTGIDLEDDSTYEIPRQIKTVIIAKPLTAFSVKGKFILDQYVMAG
                     GNVLWLVDPVIADFDSLYLGNGQFMAIDRELNIIDQMVQYGARVNSNIIQDKQCTHIN
                     VPVAKGDNFIVRPWPYNPILNNYNTDHPVTKNVDAIEGKFVSTVDTLAVPGITKTILL
                     KTSGQSRYLNAPARVNFNIVDPKFAPTDQQYNKPELPVAVLLEGNFHSAFRSIRPTAK
                     TLHDRGYGYTDTTYMAEGKPAKQIVIGDGDLIKNFVDKEGKPDMLGVNYTERYIFGNK
                     DFAMNCIEYLCDQNGLIETRAKEVKLRPLDDQRVEKDRTQWQVVNMIAPIFLLYIFSG
                     IYFFIRYRKYAA"
     gene            5346..6389
                     /locus_tag="PLM90_09075"
     CDS             5346..6389
                     /locus_tag="PLM90_09075"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HMZ89566.1"
                     /translation="MNRNWLYFGILVILCVVAYFTLIRDNSSSYSKKDAAFAIADTND
                     IYRIVLTNMKGDTIDLKRNQDDWLVNDLYAPRPDAMHNLLRTMNQMAVKVPVSKSMHN
                     NVIRNISGRHTHVQIFDAKNKLIRGYYIGDNTDQLNGTFMLMEEADMPFVVNIPGFQG
                     YISSVFFTDITDWRSKEIFAYSTAEIQQIDVTYPAVKDSTFSLVKQQNGSFLIVANKQ
                     ENKPINQEIVQFYLKQFARLNAEYYINEPDKRDSLLGGQPACLMSVTDIQKRTTTLKI
                     YYRPVTVRSKAQFTYDDKPLQFDPDKFYGIFHGDKDLAIIQNFVFGKVLVGPGFFYRQ
                     RPGGSNMLNEQAK"
     gene            complement(6457..6837)
                     /locus_tag="PLM90_09080"
     CDS             complement(6457..6837)
                     /locus_tag="PLM90_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89567.1"
                     /translation="MRLTYTLVIGLILASCAHSETETRTPVQIKEDLRRAELHSPTMY
                     LRVSGELKEDSVFCLINNTAKEADYPNAIIAVNYFNDSRQLIKSELINVGNQILKGTT
                     INFNSKVDPPDEYASVSFSVREVK"
     gene            complement(6892..8178)
                     /locus_tag="PLM90_09085"
     CDS             complement(6892..8178)
                     /locus_tag="PLM90_09085"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89568.1"
                     /translation="MKKITLVSLWLVCFISLKAQFPVISPAVETALTLTFPISVSNSG
                     NVAWNPSANLYYSCRIGNAVYPLITWNALGVQMDYDTTGQDSRGMWWNASLNQLERNC
                     FSSVGWAKINLDANSYAENTFSILFSGMLQPNAQSVGTYDPDANQVIFYNAGNCYKYD
                     RTTGLLVSTLALTGATLTTITANGIVYTGVTGAEIGIYDYSAKKVLLFNKTTGVFSAS
                     STLPATAPGPTTYGIGYANNLFWVYKTSPESKWYSYRIFDIPLPVYAIALAGNQIANA
                     NYLHWTVDADEPVVSFDLQHSSDGKTFKSIASIPGSSDGSEVYEYMHQFPAPGANYYQ
                     VISTDQQGEKHTSDIVCITSESLSSVLKVFPNPADEVISIGGLAGGMSYTIYDESGRQ
                     IASGNSNPDGTISLTDLPSGNYFLYLASTPIRFIKQ"
     gene            complement(8278..9852)
                     /locus_tag="PLM90_09090"
     CDS             complement(8278..9852)
                     /locus_tag="PLM90_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89569.1"
                     /translation="MLRKTLGFLTLLIAVNRLTLSAQDATPPFTISLEEATYSDWPGL
                     QSYVAGTWDGKWILLAGRTGGLHAFLPPNPFMPMEANKEVHVLDPLTGDHWSSSVTIL
                     PEDLSNQMQSTNPQAFQRGNTLYVIGGYGHDETAMEMRTFPALTAIDLDILGNAVING
                     TDITPAFRTIQDTIFEVTGGEIGMLGDTVYLFGGHSFTGVYSKPAGPQFTQVYTNRLT
                     KFRIVDDGTDISIADLEFITDTTAFHRRDLNFEPLMLPGEEHALIALSGVFQYEADWP
                     WHEPVVVTSGGYYVDEALYQKMNNYTCPVMSIYDADSEKSYLTLFGGFSEYWFDADDS
                     ILKQDLNIPFVHDISTVIRNTDGTMTQYIQPISLDGLLGSNAEFLINPEIPQYDDRII
                     KLTEIDGDVLAGYIFGGIDAEIPNFTPSIASNRLFKVWLHYERETPVVDLTQNGIKVY
                     PNPVMDELTIQNLAQYGITGISIADMLGNTLINQSSAIPSGYRKTISLEKLPAGVYMV
                     KVSSANGFTMQQVIKQ"
     gene            9978..11096
                     /gene="dnaN"
                     /locus_tag="PLM90_09095"
     CDS             9978..11096
                     /gene="dnaN"
                     /locus_tag="PLM90_09095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMZ89570.1"
                     /translation="MKFIVSTTALLRNLQTVNGVVSPNTVLPILEDFLFVVEKNKLTI
                     SGTDLETSITTWMDIESKSEGKVAIPARILVDYLKTLPEQPLTFSIDEESCQVEITTD
                     RGKYKLNGENGDDFPRVSSDDKLSEIALPSGVLQKVINKTAFATSNDELRPPMTGVLF
                     QLNKDGVNFVATDAHKLVRYRRTDIKADKASSLIMPKKALNLLKSNLPGEQTQVTLSY
                     NNSFAFFSYNNVKLICRLIEGRYPDYNAVIPQENPNRLTINRADFLNTLRRVIIFSNK
                     TTHQVVLKIKGNEMGVNAQDLDFSNEANERLDCAYDGDNMEIGFNAKFLLEMLNAMDN
                     EEVRFELSTPNRAGLLLPTESDEHEDLLMLVMPVMMNV"
     gene            11336..>11959
                     /locus_tag="PLM90_09100"
     CDS             11336..>11959
                     /locus_tag="PLM90_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89571.1"
                     /translation="MIINQLVFRFYSIACHKLIKKATGAALNVFTTVKPYCELLQIEL
                     IQKYEIFIVFSNPNYPKMKILGLDLGVSSIGWALIERKENETKILGGGTRIIPLSVEE
                     NDEFTRGNAISKNRNRTLKRSARRNQHRYKLRKHKLHNVLVANNMLPSSELFIGLPPL
                     TLYTMRAKAVHEQVTLTELGRILFHINQKRGYKGKPTLDNNNNSKEEE"
BASE COUNT         3653 a         2327 c         2474 g         3505 t
ORIGIN      
        1 taacgtttcg ggtattgccg aagtgggggc atttgaaaac cgtcagctga aatatagcac
       61 aaaagctgat agaagtacaa atgttgatat acttccatca gcccccattt tggcaatacc
      121 ttgttagctg cagtattttt ttattgggaa gttcctttta attcgttttg taaactcgct
      181 atcttttctt tcaggttgtt gctaattttt gaaggaaaat ctttcacctt tttaaggtca
      241 aacgaaagaa ttgccaatga aactcctaca aaaataaacg ccaacgcagt tgcggttact
      301 aatgaaattc ctgaaaatat tgggtttgcc aacagtatga atgaaaagat aattcccaat
      361 gtactggcta tggcaaggtt tccccatcgc aatattccca tacctttcat atcaaatgca
      421 attcccaata attggaatga acggaacatt accgtaaagc ctacaacgaa tggtaaaatt
      481 gttattgaaa ttcccggata cattaccaga taaatcccca ttgccaacga aaatagtccg
      541 cctactaaat accaacccca accagaaagg gttttggcat tttgaatgga aaagaaaatt
      601 tcaaacaatc ccgatgttat aaacgatata ctgaaaatta cggataatgt aacataagtt
      661 tccagcggaa ccgtaaaaat gtatatgccg aaaactacaa acaaaattcc cacgataaga
      721 ggaacgtacc aatgtttgat tgaattggtc aatgtgttta gtaaattagc cattggttaa
      781 tttttaaatg ttagtaagaa tttgctttgg ctcttgcatt aacggcatct ataactgctt
      841 cttgagcatc ggatatagca tctaatgcaa aatacacact catttcagca tcatcttcag
      901 cccattctgc actcaattct gcatcagaag cttccaaatt atatttagcc tttctaaaat
      961 cggattttaa tttcgcaaca ccgttatccc aatttgattt tacgttgtcc caatgcgatt
     1021 ttgcctcacc ttcaagcgaa gccttttttg cttcaaatgc ggctttggtt tcggcaacat
     1081 tttctttagc ttcgttttgc cattgttgaa gttgctcttt ggtttgtcct tttgaagctt
     1141 caaacttgtc ttcaagttgt tttgcttttg ctgtgagttc tgctaataac tctgataatt
     1201 tttgtgccat tgtttggaaa atttagtttg cctacttctt aatacggtgt tcggcttgtc
     1261 cgttttttta tttaagtgtt ctgtctattt ttgggtgtct atgcatattg cagctaacgc
     1321 atgcgttgag gcccctgagg gttgcgacca aggtcaaact ggatagctgt aacagtaaca
     1381 ttatatccgg tagttggggt caatgtcaat tgaatagctg aaaaagcagt agaaaatgca
     1441 cctgttgatg tgctccaggt gtcagagctg taacctgttc cacaggcgtt gatggcaaca
     1501 acaccattaa ccggtgtaag gttagaagca gttacacctg ttgcaacagt tccggcaaca
     1561 ccggtctcgt cgttggtgta tgtgtaaagc tgcgcattta ctagatcaga ctgacacatg
     1621 aggatgatac ctgtagccag cccaaggaga ttctttaatt tcattttgta tgcttttttg
     1681 gttagtgcgg gcaaaggtag gacgccgagt aagattattc acctaaaagg tgataaaatg
     1741 taaggaattg atgactttgc gacaatattt aacatattcc ggccattggc aattctataa
     1801 acagatgagc ctttggagat ttatgcatcc tataaaatag gcaataaagt attttacatg
     1861 tttacaatgc caggacttaa accttcgttt gattacctaa ttttgcggct ttcatgtcag
     1921 taaaggttca aaacatcacc aagatctacg gcgagcagcg tgcggttgac gatatctcat
     1981 ttacggtgag tccgggcgaa atacttggct ttttagggcc gaatggtgcc gggaagtcca
     2041 ctaccatgaa aattctcacc tgttttatcc ctcaaaccgg cggtgaagcc agtgtttgcg
     2101 gatttgatat caataagcaa gctgtagaag tacgcaggca aataggctat ttgccggaac
     2161 ataatccatt atataaggag atgtatgtgc aggaatatct ggagttcgct gccaatatca
     2221 atggcatgaa gaaagcatct gccaggatta acgagatgat agagataaca ggattggttc
     2281 ctgagcgaaa gaaaaaaata ggtcagctat cgaaaggata tcgtcagcgt gtgggattgg
     2341 cgcaagccat gttacatgat ccgaaggtgt tgattctgga tgaacctact tcaggtttag
     2401 acccgaatca gttggttgaa gtacgcaatc tcattaaaaa tatcggtaag gataaaacgg
     2461 taattctttc cacgcatatc atgcaggagg taactgccat ttgcaatcgc gtaatcatca
     2521 tcaacaaagg aaagcttgtt gccgatgaca gtatcagcag tttacaatcc aggcaaaaag
     2581 gcattactgt tgtgttggct gaatttaaag aaacggttga caagggtatg cttatgcgca
     2641 taaatggtgt gagcaaagta gcagcagaag gaaaggcttt gcgcatagaa tataccggac
     2701 gcgaagatat gcgcgaactg cttttcaaat ttgcagttga gcataaactc acgctattga
     2761 atttacaaat tgaaacaaaa agtctggaag acatattcca tacgcttaca aaataattta
     2821 agtacttgta tactcagcat aaaatttaaa ataaacagtg tttgccattt tcaaaaagga
     2881 attaaatgta ttcttcagtt cgctgatagg atatattgct attaccattt ttttggtggc
     2941 aaacggatta tttacttggg tgtttcccga gactaatatt ttagacgaag gttttgccac
     3001 gcttgatgca tttttcaact tcgcgccatg gattctgatg tttctgatac cggccatcac
     3061 catgcgttca tttgccgaag aaaagagcag cggcaccata gaatttatta ctacaagacc
     3121 agtcactgat attcaaataa tattaggtaa atatttaggt gcatttgtat tggttatatt
     3181 cagtatcata ccaaccatca cctattactt cgccattcgg ggtttggcgt ctccggttgg
     3241 aaatgtggat accggaggta ttattggaag ttatatcgga ttaatactat taggcgcatg
     3301 ttttgttgcg attgcgctat ttacatctgc agttaccaat aatcagatag ttgcatttat
     3361 ctccggcatg ttcgcttgct tctttttcta tctggcgttt gacagtttgt cgctcatacc
     3421 ggtatttgtc ggttccgcag atagttattt caatgcattg agtataaatc aacattatca
     3481 aaatatcagc aagggttata tcgacctgcg cgatgtggta tatttcttca gtatgatcac
     3541 cattttcata ttgatgacaa aaacagcatt gggaagcaga aaatggtaat tgattgaacg
     3601 tggacgcgaa gcttttatat aaaaagaaca gaagacggga gttgctgcaa ctactgctgc
     3661 ttactgctat aattgtaatc gtcaatgtgg tggtttcagg ggtatttttt cgcatagatc
     3721 ttactcagga aaaacgctat tcacttagca cccctacaaa agaactgctg cgcgcccaag
     3781 atgatattat tttggcacga gtttatcttg cgggcaatct cacacccaat ctgcaacgat
     3841 tacagcagtc gacagtagaa atgctcgatc agtttaagaa ttatgcggga gagaatattc
     3901 aatacgacat ttttgatccg ttcagcatac cggatgaaac caatcaaact gaactgatca
     3961 aagaactgga agataaagga atattgagtg ttcagttttt tgaaagcgcg acagatgaag
     4021 catcagtgaa atatgtattc ccttttattt cgctcaccta tcgcgacagg gaattggtat
     4081 ttcctcttat tgacagaggc accatgccat tgcctctgaa ccccgatagt gatccatcgg
     4141 tttcgatatc attactggaa tacaaattca cgaaagcaat ccgacaactt accaccgaag
     4201 agaaaccata tatcgcattt atttcagggc atggagaatt gaatcgcctg gagttgaacg
     4261 acatggccgc ttcgcttaat gaattataca atgttacggg cattgatctt gaagatgaca
     4321 gcacttatga gatcccgcgt caaataaaaa ctgtcattat tgcgaaaccg ctgacagctt
     4381 tttcagtaaa agggaaattt attctagatc aatatgtaat ggccggtgga aatgttctct
     4441 ggcttgttga tccggtaatt gcagattttg atagtttata tctcggcaat ggtcaattta
     4501 tggctattga cagagaactc aatattattg accagatggt gcaatatggt gcccgggtaa
     4561 acagtaatat tattcaggac aaacaatgca cgcatatcaa tgtgcctgtt gcgaaaggtg
     4621 ataattttat cgtacgacca tggccctata atcctatatt aaacaattac aataccgacc
     4681 atcccgtaac aaaaaatgtc gacgccatag aaggtaaatt tgtgagcacc gttgacacgc
     4741 ttgcagttcc gggaattaca aaaacgatac tcttaaaaac aagcggacag agtcgctatt
     4801 taaatgcgcc tgccagggtg aatttcaata ttgtagatcc taaatttgcg cctaccgatc
     4861 agcaatacaa taaaccggaa ttaccggttg ctgtgctgtt ggaaggcaat tttcattccg
     4921 cattcaggag cattcgtcca actgcaaaaa cattacatga cagaggttac ggctataccg
     4981 acaccactta catggcagaa ggaaaacccg caaagcagat tgtaattggc gatggtgatc
     5041 ttattaaaaa ttttgtagac aaggaaggta aacccgacat gcttggtgtg aattatacgg
     5101 agcgctatat tttcggcaac aaggatttcg ctatgaattg cattgaatat ttatgcgatc
     5161 agaacggatt gattgaaacg cgcgccaaag aggtgaaatt gcgcccgctg gatgaccaac
     5221 gcgtggaaaa agatcgcacg caatggcaag tggtgaatat gatcgctcca atatttttat
     5281 tatacatttt cagtggcatt tatttcttta tacgatatcg caaatacgca gcataaataa
     5341 tatcaatgaa ccgcaattgg ctatattttg gaatcctggt tattttatgt gttgtcgcct
     5401 attttacatt aataagagac aactcttctt cgtacagtaa aaaggatgca gcatttgcta
     5461 ttgccgacac aaatgatatt taccgcatcg tgcttaccaa tatgaaaggc gacacgatag
     5521 atctgaagcg aaatcaagat gattggctgg taaacgatct ttatgctcca aggcctgatg
     5581 ccatgcacaa tttattgcgc accatgaatc aaatggccgt gaaggttccg gtatcgaaat
     5641 ccatgcacaa taatgttatc agaaatatta gcggcaggca tacgcatgtt cagattttcg
     5701 atgcaaaaaa taaattgatc agaggatatt atatcggcga taataccgac cagttaaatg
     5761 gcactttcat gctcatggaa gaagccgaca tgccatttgt ggtaaatatt cccggttttc
     5821 aaggatatat ttcaagcgta ttttttaccg acatcaccga ttggcgcagc aaagaaatat
     5881 ttgcttacag cacagctgaa attcaacaaa ttgatgtcac ctatccggca gtaaaggatt
     5941 caacattttc attggtgaaa caacaaaacg gcagttttct cattgttgcc aacaagcaag
     6001 aaaacaaacc aattaatcag gagattgtgc aattttattt aaagcagttt gccagattga
     6061 atgccgaata ttacatcaac gaacccgaca agcgcgactc attattaggt gggcaaccgg
     6121 catgtttaat gtctgtaaca gatattcaaa agcgcacaac cacgcttaaa atttactatc
     6181 gtccggttac ggttcgaagc aaggctcaat ttacttatga tgataagccg cttcaatttg
     6241 atccggataa attctacggc atttttcatg gcgataagga ccttgctatc atccaaaatt
     6301 tcgtattcgg taaggtgctg gtaggtccgg gctttttcta tcgacaacgc ccgggtggca
     6361 gcaatatgct taatgaacaa gcgaagtaat acaacttcct cggttcatgc tgaaagcata
     6421 aaggatataa aaaagtaatt gcttccttga aaattattat ttcacttctc ttacagaaaa
     6481 tgacaccgaa gcatattcat ccggagggtc aacctttgaa ttgaagttga ttgtggtgcc
     6541 tttcagtatc tgattgccaa catttatcaa ttcgcttttt atcaattgcc ttgagtcatt
     6601 gaagtaattt acagcaatga tggcattagg ataatccgct tcttttgcag tgttgttgat
     6661 caggcaaaat actgaatctt ctttcagttc accactcaca cgtaaataca tggttggcga
     6721 atgcagttca gctctgcgta ggtcttcttt gatctggact ggtgtgcgcg tttccgtttc
     6781 gctatgggcg caagaagcca agatcagacc tatgacaagg gtataggtta atctcattgt
     6841 gtgtgtttag gaatactaaa ttaacacatt tgtcacaaat accctaattt ttcactgttt
     6901 tatgaagcga ataggtgtgc tggcgaggta taggaagtaa ttaccggaag gaagatctgt
     6961 aagtgagata gtgccgtccg gattgctgtt gccgctggct atttgtcttc cggactcgtc
     7021 ataaatggta taagacatac cacctgccaa tccaccaata ctgataactt catctgccgg
     7081 attagggaaa accttcaaca cactgctcag cgattcgctg gtaatacaca cgatatcaga
     7141 ggtatgcttt tcgccttgct gatcggttga gattacctga taataatttg ctcccggagc
     7201 cggaaattga tgcatatatt cataaacttc agagccatca gagctaccgg gaatggaagc
     7261 aatactctta aatgtttttc catcagacga atgttgcaga tcaaatgaaa caacaggctc
     7321 atctgcatca actgtccaat gcaaatagtt agcattggca atttgatttc ccgctaatgc
     7381 aatagcatat acaggtaagg ggatatcaaa aattctataa gaataccatt tactttcagg
     7441 ggatgtttta tacacccaaa aaagattatt ggcatatccg ataccgtatg ttgtgggccc
     7501 aggcgcggtt gcaggtagcg ttgaactggc ggaaaacaca ccggttgttt tattaaacaa
     7561 caatactttt tttgctgaat agtcatatat tccaatttca gctcccgtaa caccagtata
     7621 aacaatacca ttggctgtaa tggtggttag cgtggcaccg gttaatgcca gggtagatac
     7681 tagcaatccg gttgtgcgat cgtatttata acagttaccg gcattgtaaa aaatcacttg
     7741 attggcatca ggatcatatg ttccgacact ttgtgcattc ggttgtaaca taccactgaa
     7801 taatatgctg aatgtatttt ctgcataact attcgcatcc aaatttatct tcgcccaacc
     7861 aacagacgaa aaacaatttc tttccagttg attcaaactt gcattccacc acatgcctct
     7921 gctgtcttgt ccggttgtgt cataatccat ttgcacacct aatgcattcc aggttattaa
     7981 aggataaacg gcattgccta ttctacacga ataataaaga ttagccgatg gattccaggc
     8041 tacatttccg gagttgctta ctgaaatggg aaaggttagc gttaaagcgg tttcaacagc
     8101 aggtgaaata acaggaaact gtgctttgag tgaaataaaa catactagcc aaagcgatac
     8161 gagtgtaatt tttttcattg gaatcaagtg attttgtgac ataatttttg atgtcacaaa
     8221 aggacctgtt tccgtgcctt tctgaaatac cccggtcggg tgattttagt aactccccta
     8281 ttgtttgatt acctgttgca tggtgaaacc gtttgcagac gacactttta ccatgtaaac
     8341 accggccgga agtttttcca gagaaatagt ttttctgtaa ccggacggta tagcggatga
     8401 ttggttaatg agtgtattgc ccagcatatc tgcaatagaa atgcctgtga tgccatactg
     8461 tgcaaggttt tgtatagtaa gctcatccat caccggattg gggtacactt tgataccatt
     8521 ttgcgttaaa tcaacaacag gtgtttctct ttcgtagtgc agccatactt tgaacagtct
     8581 attcgaggcg atagagggtg tgaaattcgg tatctctgca tctatccccc cgaaaatata
     8641 tcctgctaaa acatcaccat caatctcggt aagtttgatg atgcgatcat cgtattgcgg
     8701 aatttcggga ttgattaaaa attctgcatt agatcccaac aaaccatcca aagagatcgg
     8761 ttgaatatat tgtgtcatgg tgccatcggt atttcttata acggtagaaa tatcgtgcac
     8821 gaaaggaata ttcaaatcct gttttagaat agaatcatca gcatcaaacc aatattcact
     8881 gaatccgccg aacaaggtta aataagactt ttctgaatcc gcatcataaa tagacattac
     8941 cgggcaggta tagttattca ttttctgata caaggcctca tcaacataat aaccgccgga
     9001 agttactaca acaggttcat gccatggcca atcggcttca tattggaaaa caccgctcaa
     9061 cgcaattagc gcatgctctt ctccgggaag catcaatggc tcgaaattca ggtcgcgacg
     9121 gtgaaatgcc gttgtatctg ttatgaattc aaggtcggca atggatatgt cagtgccatc
     9181 atctactatg cggaatttgg tgaggcggtt ggtatagacc tgtgtaaact gaggccctgc
     9241 aggttttgaa tacactccgg taaatgagtg acctccaaat aaatacacgg tatcgcccaa
     9301 catgccgatt tcaccacctg tcacctcaaa tatggtatcc tgtatggttc taaatgcggg
     9361 ggtgatgtca gtaccattta taaccgcatt ccctaatata tcgaggtcga tagccgtaag
     9421 tgcagggaaa gtgcgcattt ccatggctgt ttcgtcgtga ccataccctc ctatgacata
     9481 taaagtgttt ccacgctgaa aagcttgtgg attggtgctt tgcatctgat tggagaggtc
     9541 ttcgggaaga atggttacac tgctgctcca atgatctccg gtaagcggat ccagcacatg
     9601 cacctcctta ttggcctcca taggcataaa cggatttggg ggtaaaaagg catgcaaacc
     9661 ccctgttcta cctgccaata atatccattt cccatcccag gttcctgcaa cataggattg
     9721 caatcccggc caatcgctgt aggttgcctc ttcaagcgaa atggtaaaag gaggagtagc
     9781 atcctgtgcc gaaagggtta agcggttaac tgctatcaac agcgtaagaa agccaagggt
     9841 tttgcgcaac atggaacaaa gataaggtgc gtgggcattg catttggtga cttttagaac
     9901 agtacagtgc tatccacccc ttgttgaaaa acattcttac tgtttgttta cctttgcctg
     9961 tattcaaaaa gtagtacatg aagtttattg taagcacaac agccctattg cgcaatctgc
    10021 aaaccgttaa cggtgtggta agccccaaca ctgttttacc tatactggaa gattttcttt
    10081 ttgttgttga aaagaacaaa ctgaccattt cgggaactga ccttgaaaca tctataacca
    10141 cttggatgga cattgagtca aaatcggaag gaaaagttgc cataccggcg cgtattcttg
    10201 ttgattatct gaagacactt cccgagcaac cattgacatt tagcatagat gaagaatctt
    10261 gtcaggtaga gattactacc gatcgcggta aatacaaatt aaatggcgaa aatggcgatg
    10321 attttccacg cgtttcaagc gatgataaat tatcggaaat tgcattgcca tcgggtgtat
    10381 tgcaaaaggt gatcaacaaa acagcttttg ctaccagcaa tgatgaattg cgtccgccga
    10441 tgacaggtgt gctctttcaa ttaaataaag atggtgtaaa ctttgttgct accgatgcac
    10501 acaaactggt gcgttaccgc agaacagata ttaaggccga caaagcatca tcgcttatca
    10561 tgcctaaaaa agctttgaac ttattgaagt caaatcttcc gggtgaacaa acacaggtga
    10621 cattatcata caataattca tttgcatttt tcagctacaa taatgtgaag ttgatttgtc
    10681 gactcattga aggtcgctac ccagattata acgccgttat tccgcaggaa aatccaaatc
    10741 gccttaccat caatcgcgca gattttctca atacattacg tcgtgtgatc atcttctcta
    10801 ataaaacaac gcatcaggtt gtattgaaaa tcaaaggcaa cgaaatgggt gttaatgcgc
    10861 aggatcttga tttcagcaat gaagcgaacg agcgtttaga ttgtgcttat gatggcgaca
    10921 atatggaaat tggtttcaat gcgaaattct tattagaaat gttgaatgcc atggataatg
    10981 aagaagtgcg ttttgaactt tccacgccta atcgtgcagg attattatta ccaactgaaa
    11041 gcgatgagca tgaagatttg ctgatgttgg tgatgccggt gatgatgaac gtgtagggga
    11101 ggagaaagga gaaaggagag aggagaaagc ttgcctcgcc gataggcggg tggtgagttt
    11161 ttttgaagta attgaaagta aaaccccgat ctggtcagat cggggtttta cttttaatac
    11221 aggatcaatt gcattgatag tttttcatag atgaactaat tatagcattc aaggacaaaa
    11281 tgggcagacg tactatttaa gtcacaaaat ttaggcaatt aaaaatatca actatttgat
    11341 tatcaatcaa ttagtttttc gtttctacag tattgcttgt cacaaactaa taaaaaaagc
    11401 gaccggagcc gctttaaatg ttttcacaac ggtaaaacct tattgtgaat tgcttcaaat
    11461 tgagttaata caaaaatatg aaatatttat agtattttca aatccaaatt atccaaaaat
    11521 gaaaatactt ggtttagact tgggtgtaag ctcgataggc tgggcgctca tcgaaaggaa
    11581 ggagaatgaa acaaaaattc taggaggtgg tactagaata attccgctat cagttgagga
    11641 aaatgatgaa tttacacgcg gaaatgcaat atcaaaaaat cgaaatcgca cgttaaaacg
    11701 ttctgcaaga cgaaatcaac ataggtataa gcttaggaaa cataaattgc acaatgtact
    11761 tgtagctaat aacatgcttc catcgtcaga attattcatt ggtctccccc cgctaacact
    11821 ttatacaatg cgggcaaaag cagttcatga acaagttaca ctcacagagc taggcagaat
    11881 tttgtttcac attaaccaaa aacgaggtta taaaggcaaa cccacattag acaacaacaa
    11941 taattcaaag gaagaagaa
//
LOCUS       DAOQOU010000095        11941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2750_length_11941_cov_6.609541, whole genome shotgun sequence.
ACCESSION   DAOQOU010000095 DAOQOU010000000
VERSION     DAOQOU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2750_length_11941_cov_6.609541"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="PLM90_09105"
     CDS             <1..1683
                     /locus_tag="PLM90_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ89572.1"
                     /translation="EVKQVANFNHTLYAQVADTIYQYDGTSWMPWLYEENWHTNSISV
                     DEQAMVVTQVFGINSPADSSRIITLLADGTITDLTNDVDLLFPSEATLGDDGELWIAD
                     LYRGLLAYDGAQFKSYLPNGPGTSDVTDMKSAGNVLYVAPGEINASWNYQYNHAGFFA
                     YDYGFWNTLNQYGFGILDSVYDFITVDVDKKNNVVYMGSFGGGLVAYDRSTGVMQLYK
                     QGYLENAVGDPGSYRVGGLAVDSSSNLWISNFGSIDPIAVRKADGTWAHFNPGLTADV
                     FNQLGQIAIDEFNTKWIQLPRGNGILVFNEQGTIDDQSDDLIKVLGAGAGNGNLHTNY
                     INCIAVDKQSEIWVGTSEGITIFYNPSEVYTGTTAGDATQPLVNLGGYYEQLLRNDIV
                     NTIAVDGANRKWVGTNSGAFLISADGTEQLLYFNSDNSPLISNTVLNIEIDGTTGDVY
                     FGTDKGIISYRYTATEGVGEITSVKAFPNPVREDYTGSIAISGLTADAEVRITDMAGR
                     TIYQTIALGGQAVWDGNGYDGKRAATGVYMVYASNEDGSETAVSKILVISSK"
     gene            1771..2487
                     /gene="recO"
                     /locus_tag="PLM90_09110"
     CDS             1771..2487
                     /gene="recO"
                     /locus_tag="PLM90_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMZ89573.1"
                     /translation="MLHKTRGIVLRTTRYGDTSVIAKVYTEAFGLQSYIVNGVHTSKN
                     TGKGSLYQHGNLLEMVVYHKEQGALMRISECRFSHIYEQLPFNVIKGGLLLFYIELLN
                     RIIREEEQNESLFDFLFESFIDLDQTDKKIGAHHIWFLLGLSKYLGFYPNICTGHIFD
                     LEEGAFTQLMPRHTNYISENGAMYLRNCIDPELKNYDQMQIPASDRRSLVHWLINYYK
                     LHLSDFGEVRSLDVLEGLFR"
     gene            complement(2495..3223)
                     /locus_tag="PLM90_09115"
     CDS             complement(2495..3223)
                     /locus_tag="PLM90_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ89574.1"
                     /translation="MSLLPWQNATVVDVVAHNAHTKSYFLAAEGVEVFAFKPGQFVTF
                     DLPIADRPSQRMRSYSIASAPGNNNVFELVVTHKPNGAGSGYFFDHCSIGHQLKFRGP
                     QGTFVLPDSFNLPIALICTGTGVAPFRSQVKYLLENNITIPEIHLVFGTRTLSDALYK
                     EEFESLAAAHPHFHYHLTLSRPDHNWKGKHGYVHELYTDICKNGTVDMDFFLCGWRMM
                     VNEARTRLQDLGYPKERVHLEVYD"
     gene            3358..6756
                     /gene="mfd"
                     /locus_tag="PLM90_09120"
     CDS             3358..6756
                     /gene="mfd"
                     /locus_tag="PLM90_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HMZ89575.1"
                     /translation="MEWLALPEKYREHDDRIKQLHEQIGEKPQNLHLKGIAGSLPSFV
                     ASAIYKKQQRHHLFILNDKEEAGYFLNDIETLFPGKDVLFIPDSFKKQNSFDEISTNN
                     ILQRTEAINRIAHSHTRGEIIITYPEALVEKFVIPEALEKQIIYIKKGEKLDDQFIID
                     VLIENGFDRNDFVYEPGQFSVRGGIIDIFSFGNDLPYRIELFDDEVESIRVFDPASQI
                     SQKMIAQVTIVPNIQTQFREEQKASLLNTLPKDTLVWCKDARYTFEVINQVYEKAHDW
                     YAVVSKENMLHVDHPFRQHPPQELLLSADDLLSEVPGFSWIEFGLNSSFPDARTIVFD
                     TVPQPLFNRNFETLIAQLKELRSKRYDIFIFMSNARQAERFHEIFTDLNAKVDYTPVI
                     PIIKEDGKPSTTIAQGFIDHDMHVVCFTDHQLFERHHSYRVKSGYSKNDAVTLKTLME
                     LRPGDYVTHIDHGVGVYSGLEKIEVNGQIQEAVRLVYRDNDLLYVNINSLHKISKYVG
                     KEGTEPKLNKIGSDAWEQLKRKTKTRVKDIAKDLIQLYAKRKAAKGFAYSQDGYLQNE
                     LEASFIYEDTPDQNRATVDVKRDMEKPYPMDRLVCGDVGFGKTEIAIRAAFKAVCDGK
                     QVAVLVPTTILATQHYKTFSERLKDFPVRVDYINRFKSDKEKKQTLELLEAGKIDILV
                     GTHAIIGKKIKFKDLGLLIVDEEQKFGVTVKEKLRAFRANIDTLTLTATPIPRTLQFS
                     LMGARDLSIINTPPPNRQPIQTEIKSFDADFIRDAIYHEIYRGGQVYFIHNRVRDIEE
                     TAALIKKICPDLHVGVAHGQMEGDVLEDHMMQFITRNYDVLVCTNIVESGLDIPNANT
                     IIINNAHHFGLSDLHQLRGRVGRSNKKAYCYLISPPVFALPDDSRKRLKTIEQFADLG
                     SGFQIAMRDLDIRGAGNLLGGEQSGFIAEIGYDVYHKILDEAIRELKQTQFKEIFADQ
                     VHEKQEYVRDVQIDTDLEMLIPDEYISSISERMTIYTRLDNISNEEQVTAFKNELADR
                     FGKLPKQVEELFNGIRLRWLAKDLGMERIIFKSRKLRCYFVENSASVFYDSPLFQNIM
                     QAIAQKRSPGTIKQTDKHLILVFEQVQSMQHAKSLLEALTKAVV"
     gene            6803..7339
                     /locus_tag="PLM90_09125"
     CDS             6803..7339
                     /locus_tag="PLM90_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ89576.1"
                     /translation="MILETPRLILREVTPDDVDGFYKLDSDAEVHRYLGNQPVTSLQQ
                     CKDVIAFVMKQYVDNGIGRWAVVEKFSGDFIGWAGLKLVKEETNGHVNYYDYGYRLIR
                     QYWGKGYASECSPFILQYGFTHLPADIIYGAAHVNNIASNRILRKNGFQFKNTFFYES
                     DLCNWYALEKSLFEGNLP"
     gene            7434..9497
                     /locus_tag="PLM90_09130"
     CDS             7434..9497
                     /locus_tag="PLM90_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ89577.1"
                     /translation="MSTQTVRLTAEFKKQTTRVVIVILLFVLVYLILIALAVGLTAAS
                     IAGGIFIILASPAFITLVLGVGMASLGSLILIFLLKFLFTSNKYDRSHLIEVTRAEEP
                     ALFDMIDDIVEQVKTAKPKKVYWSADVNAAVFYDSSFWSMFLPIRKNLIIGIGLLNTL
                     TAEELKAVLSHEFGHFSQRTMKVGSYVYNVNKVIFNMLYDNDSFGSMASRWGSVSGYI
                     AIFVMLAFHIINGIQYLLRKMYELVNKSYSSLSREMEFHADEIAASITGYEPLKTALM
                     RFSISSASYNEVLNYYNEKASQNIKSENIYADQTLVLKFLAEINKIRVENDLPNVTEE
                     EQNRFNKSRLVIKDQWASHPTTKDRVTRLENTGFHASGLQHQPAKTLLRNAGNTYKQV
                     TANLFSNVTYKGNTSFESPETLITAYREKFESNAFPAIFNSYYDDKNIEVFNPGEVTP
                     STETILMEELYSDKAVESVYAEIGLASDINVLKDIGNGFTQVKSFDFDGIRYSRKKAL
                     TLVDNLKPELDKLTAENKAHDIRIYATFISLEQKLGLAPKLQTMYQTLFDYAADLERK
                     LQIYSHLASKLEFVSVQTSHEEIDRRLALVRPLEDKLKTEINKILSDTFYQSEITEDM
                     MKNMQSFTSRHIEYFLLGNYVEENLSLLYKAMNNYRYLVLRAYFILKRNILLYQAELH
                     QKTVA"
     gene            9602..10294
                     /locus_tag="PLM90_09135"
     CDS             9602..10294
                     /locus_tag="PLM90_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89578.1"
                     /translation="MKSMITGAAALIFFLTTAFYPGDNCESYFANKVGTSMEQTSYDA
                     KGKMTSKSVSTVKAYDAIDGGFASTLNVRVMDAKDKEVSNSDVIMKCQQDKFYMDLSN
                     AFPKEMAGMEGAEVEMENTFVEFPAHPVAGQSLPDQTSSMTIKLNGTPFMTMTINTTN
                     RKVEGFESMTTTAGTFECMKYSADTEVKSMFTSKSHSVMWMSKNVGLVKMESYDDKGK
                     LQATQMLTKFSE"
     gene            complement(10330..11574)
                     /locus_tag="PLM90_09140"
     CDS             complement(10330..11574)
                     /locus_tag="PLM90_09140"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HMZ89579.1"
                     /translation="MEQHKFRPESLMMSFGYKPELSEGAVKCPIFQTSTFVFRNAEAG
                     KRFFEVAYGLSSPEPGEEQGLIYSRLNNPDLEILENRLTLWDEAEACAVFSSGMSAIS
                     TVLLEFLQPGQYVLFSAPVYGGTDHFIKHVLPKWGIGHAAFDANSSEEEIHAIIQEKG
                     ADALGLIYVETPANPTNALIDMEMCKRIADVYSNDKKVYLAVDNTYMGPLWSHPLKHG
                     ADIVMYSATKYIGGHSDVIAGAVLGGNEVMTRIKTMRTFMGNMMSPNTGWLLMRSLET
                     LKPRMELQAQNAMKVVEFLNEHPKVEKVYYLGLIPEGTPEYHIYRNQYSSPGAMLSFD
                     IIGGEKEAFTFLNSLKLIKLAVSLGSTESLAEHPATMTHADVSPEDKQKLGISEKLIR
                     LSVGVEYYEDLIFDIERALSHV"
BASE COUNT         3654 a         2253 c         2590 g         3444 t
ORIGIN      
        1 gaagtgaaac aagttgcaaa ttttaatcat acgctttatg ctcaggttgc cgataccatt
       61 tatcaatacg acggcacttc ttggatgccc tggctgtatg aagaaaattg gcataccaat
      121 tccatttctg ttgatgagca ggcgatggtt gttacgcagg ttttcggaat taattctccc
      181 gcagactcta gtcgtattat cacactttta gccgacggca ccataacgga tttaaccaat
      241 gatgttgatc tgcttttccc gagtgaagca acacttggag atgatggtga gctatggatt
      301 gccgatcttt atagggggct attagcatat gatggcgcgc agtttaaatc gtatttgcca
      361 aatggtcccg gcaccagcga tgttaccgat atgaaaagtg caggcaatgt actttatgtt
      421 gctcccgggg aaataaatgc ctcttggaat tatcaatata atcacgcagg ttttttcgcc
      481 tatgattacg gattctggaa tacgcttaat caatatggtt tcggtattct ggatagtgtt
      541 tatgatttta tcaccgttga tgtagataag aaaaataatg tcgtttatat gggatcattc
      601 ggtggcggtc ttgttgccta tgatcgctca acgggagtta tgcaattgta taaacagggc
      661 tatctcgaaa atgcagtggg cgatccgggg tcttatcgcg ttggagggtt ggcagtggat
      721 tcgagcagca atttatggat atcgaatttt ggatcgattg acccaatagc cgtgcgtaaa
      781 gctgatggta cctgggcaca ttttaatccg ggattaaccg cggatgtatt taatcagttg
      841 ggacaaatag ccattgatga atttaatacg aaatggatac aattacctcg tggtaatggt
      901 attctcgtat ttaatgaaca aggcaccatc gacgatcaat cggatgattt aataaaagtg
      961 ctgggtgccg gagccggaaa tggaaatctc cataccaatt atatcaactg tattgccgtt
     1021 gataaacaaa gtgagatctg ggtgggcacg agtgaaggca ttacaatttt ttataatcct
     1081 tctgaagtgt ataccggaac aacagcaggc gatgcaacac aaccattggt taatcttggg
     1141 ggatattacg agcaattatt gcgcaatgat attgtaaata cgattgcagt tgacggagca
     1201 aacaggaaat gggtgggcac caacagcggc gcatttttga tcagcgctga cggcactgaa
     1261 caattattat attttaattc cgataactca ccgttaatat cgaatacggt attaaatatt
     1321 gaaatagacg gaactacggg tgatgtttat ttcggtaccg ataagggtat tatttcatat
     1381 cgttatacgg caactgaagg agtgggagaa ataacaagtg taaaggcatt tccaaatccg
     1441 gtaagagagg attatacagg gagcatcgca attagcggac tcacagcaga tgctgaagta
     1501 cgcataacag atatggcggg ccgaacaatt tatcaaacca ttgcgcttgg tggtcaggca
     1561 gtgtgggatg gcaacggata tgatggtaaa cgcgcagcta caggtgttta catggtgtat
     1621 gcttctaatg aggatggcag cgaaacagcg gtttccaaaa tacttgtaat ttcttcaaaa
     1681 taatttcagc ttatttgttt acccgatttg ttgaaataaa tcgcaacatt aatttcggct
     1741 attagctagc tttacaaaaa gttttttccc ttgctgcata aaaccagagg catagttttg
     1801 cgcaccacaa gatatggtga taccagtgtg atagcaaagg tatataccga agcttttgga
     1861 ctgcagagct atattgtaaa cggtgttcat acgtcaaaaa acacaggaaa gggttcctta
     1921 tatcaacacg gaaatctgct cgaaatggta gtgtatcaca aagagcaagg ggcattgatg
     1981 cgcattagtg aatgtcgatt ctcgcatatt tacgagcaac taccctttaa tgtaattaaa
     2041 ggtggcttgt tattgtttta tatagaattg ttaaatcgca ttattcgcga agaagaacaa
     2101 aacgaatcat tgtttgattt tttattcgag agttttatcg atcttgatca aacggataaa
     2161 aaaattggtg cacatcatat ctggttttta ttgggactga gtaaatacct tggattttac
     2221 cctaacattt gtaccggcca tatatttgat ttggaggaag gcgcctttac ccaattaatg
     2281 ccgcgacaca ccaattatat ttctgaaaac ggagctatgt atcttcgaaa ttgtatagac
     2341 ccggaactaa aaaattacga ccagatgcaa attcctgctt cggatagaag aagcctggtg
     2401 cattggttaa ttaattacta caaattgcat ttaagcgatt tcggagaagt acgttccctt
     2461 gatgtgctgg agggattgtt tcggtagcat tatatcaatc atatacttcc agatgcacgc
     2521 gttcttttgg gtagcctaaa tcttgcaggc gggttcgtgc ttcattcacc atcatgcgcc
     2581 agccacacag gaaaaaatcc atatctaccg taccattttt acaaatatca gtatataact
     2641 catgcacata accgtgcttg cctttccagt tgtgatcagg cctgcttaag gtaaggtgat
     2701 agtgaaaatg cggatgtgct gctgcaagcg attcaaactc ttctttataa agtgcgtcgc
     2761 ttaaagtgcg ggttccgaaa actaaatgta tctcgggaat agtgatgtta ttctctaaaa
     2821 gatatttcac ctgtgagcga aatggcgcaa caccggtgcc tgtgcaaatc aaagctatag
     2881 gcagattaaa tgaatcgggc aaaacaaatg taccttgcgg tccgcggaat tttaattgat
     2941 gtccgatgct gcaatgatca aagaaataac cggatcctgc gccattgggc ttatgtgtaa
     3001 caactaattc aaatacatta ttatttccgg gagctgaggc tatggaatag ctgcgcatgc
     3061 gttgacttgg tctgtcggct ataggtaggt cgaaggttac aaattgtccc ggtttaaatg
     3121 caaacacctc aacaccctca gctgcgagga aatagctctt tgtgtgtgca ttgtgggcaa
     3181 caacatcaac cactgttgca ttttgccatg gtaataaaga cataatgcaa aggtaaggtc
     3241 ttgaagtagt aaaaagttca ttaaataatg aaaactgact ttaattcgga ccaaattaat
     3301 tgcacatgga aactaattcc agcaagtcaa acggcatttc ttatctttgc acctttcatg
     3361 gaatggttag cgcttcccga aaaatacagg gagcatgatg ataggattaa gcagttacat
     3421 gaacagatag gagaaaagcc tcaaaacctc catctgaagg gcattgcagg ctctttaccg
     3481 tcgtttgtag cctccgcaat ttataaaaaa cagcaacgtc accacctgtt tattctcaac
     3541 gataaggaag aagccgggta ttttttgaat gatattgaaa cattgtttcc tggaaaggac
     3601 gtattgttta ttcctgattc gtttaaaaag caaaatagtt ttgatgagat cagcacaaac
     3661 aatatccttc aacgcacaga ggcaattaac agaatagcac attcacatac tcggggtgaa
     3721 atcattatca cctatcctga ggctttggtc gaaaaatttg ttattcctga ggcgcttgaa
     3781 aaacagatca tctacataaa aaaaggcgaa aagcttgatg atcagtttat catcgatgtg
     3841 ctcattgaaa acggattcga tcgcaacgat tttgtatatg aacccggcca gttttcggta
     3901 cgaggaggaa tcatagatat tttttctttc ggcaacgacc tgccctatcg catagaatta
     3961 tttgacgatg aggttgaaag tatacgtgtt ttcgaccccg cttcgcagat atcgcaaaaa
     4021 atgattgcgc aggtaactat cgtgcctaat attcaaacac agttccgcga agaacaaaag
     4081 gcttcactat taaatacact accgaaggat acactcgttt ggtgtaaaga tgcaagatat
     4141 acctttgaag taataaatca agtttacgag aaagcgcacg attggtatgc ggttgtcagc
     4201 aaggaaaaca tgctgcatgt tgatcatcca ttccgacaac acccgccgca ggagttattg
     4261 ctgagtgcag atgatttgct gagcgaagta cctggattca gttggataga attcggactc
     4321 aacagtagtt ttccggatgc ccgtaccatt gtttttgaca ccgttccgca acctttgttt
     4381 aacagaaatt tcgaaacact tatcgcgcaa ctaaaagaac tgcgcagtaa gcgatacgat
     4441 atttttattt tcatgagtaa tgcacgtcag gcggagcgtt ttcatgaaat attcaccgat
     4501 ctgaatgcta aagttgatta tactccggta attccaatta tcaaggagga tggcaaacct
     4561 tcaaccacta ttgcacaggg atttattgat cacgatatgc atgtggtctg ttttaccgac
     4621 catcagctat ttgagcgaca tcatagctat cgcgtaaaat ccggttattc aaaaaatgat
     4681 gcagttaccc tgaaaacatt gatggagttg cgtccgggag attatgtaac acatatcgat
     4741 catggcgtgg gcgtgtattc gggtttggag aaaattgagg ttaacggtca gatacaggag
     4801 gcggtgcgtc tggtgtatcg cgacaatgat ctgttatacg taaacatcaa tagtttacat
     4861 aaaatctcta aatatgtagg caaggaagga acagagccga aattaaataa aattggaagc
     4921 gacgcatggg aacaactcaa gcgtaaaaca aaaacacgtg taaaagatat tgctaaagac
     4981 ctgattcaat tatatgcgaa acgaaaagcg gctaaaggat ttgcatatag tcaggatgga
     5041 taccttcaaa atgaacttga ggcaagcttt atatatgaag atacacctga tcaaaaccgc
     5101 gctactgttg atgtaaaacg cgatatggaa aaaccctatc ctatggatag actggtttgt
     5161 ggcgatgtgg gcttcgggaa aacagaaatt gctatccgcg cagcatttaa ggccgtatgt
     5221 gatggtaaac aagttgctgt tttggtgcct accactattt tggctacaca acattataaa
     5281 acattttccg agcgactgaa agattttccc gtaagagttg attacatcaa tcgatttaaa
     5341 tctgacaaag agaaaaagca aacactagaa ttattggaag ccggtaaaat tgacatttta
     5401 gtcggcacac atgccatcat aggaaagaaa attaaattca aagatctggg tttgctcatt
     5461 gtagatgaag aacagaaatt cggtgtaacg gtaaaggaaa aattgcgcgc ctttagagct
     5521 aatattgata cactgacatt gacggcaaca ccaattcctc gaaccttgca gttttcgttg
     5581 atgggggcac gcgatctgag tattattaac acgccacctc ccaatcgtca gcccattcaa
     5641 actgaaatta aatcgtttga tgctgatttt atcagagatg ccatttatca tgaaatttat
     5701 cgcggcggac aagtttactt tattcataat cgggtaagag atattgaaga aactgcagct
     5761 ctcatcaaaa aaatatgccc cgatttgcat gtcggtgttg cacatggtca aatggaagga
     5821 gatgtgttgg aagatcatat gatgcaattc atcaccagga attacgatgt attggtgtgt
     5881 accaatattg tggaaagcgg acttgacatt cccaatgcaa atactatcat tatcaataat
     5941 gcgcatcatt tcgggttgag cgacttgcat caattgcgcg gcagggtagg gaggagcaat
     6001 aaaaaagcat attgctacct catatcacca ccggtttttg ctcttccgga tgattccagg
     6061 aaacggctca aaacaattga gcagtttgct gatctgggaa gcggtttcca aatcgccatg
     6121 cgcgatctcg acattcgtgg agcggggaat ttgctgggcg gtgaacaaag tggatttatt
     6181 gctgaaattg gttatgatgt atatcataaa atacttgatg aagccatacg tgaattaaag
     6241 caaacacaat tcaaagaaat ctttgccgat caggtgcacg aaaaacaaga gtacgtgcgc
     6301 gatgtgcaaa ttgacacaga tcttgaaatg ttgatacccg atgaatacat ctcaagcatc
     6361 tcggaacgca tgaccattta tacaagactg gataatatct caaatgaaga acaagtaaca
     6421 gcttttaaaa acgagctggc agatcgcttc ggaaaattgc caaaacaggt ggaagagttg
     6481 tttaacggaa ttcgccttcg ttggttggca aaagacctgg gtatggaacg gattattttt
     6541 aaaagcagaa aactcagatg ctattttgtc gagaattcgg cttctgtatt ttatgacagt
     6601 ccgctgttcc aaaatattat gcaggctatt gctcagaaga gaagtccggg cacaataaaa
     6661 caaaccgaca agcatctgat tttagtattc gagcaggtgc aaagcatgca acatgctaaa
     6721 tcattgctcg aagcattaac aaaagcagtt gtctgacaat actgctgtta tagccatttc
     6781 tataaattcg gtaaattagt atatgattct agaaacaccg cgacttattc ttagagaagt
     6841 cactccggat gacgtcgacg gtttttataa acttgattcc gatgctgaag tgcaccgcta
     6901 cttgggtaat cagccggtaa caagcctgca acaatgcaaa gacgtcatcg cctttgtgat
     6961 gaagcagtat gtagacaatg gcattggtcg ctgggctgta gttgaaaagt ttagtggtga
     7021 ttttatcggc tgggccggat tgaaattggt gaaggaagaa accaatggtc acgtgaatta
     7081 ttatgactat ggatatcgcc tcatacgcca atattggggt aaaggctacg cttcagaatg
     7141 cagtccgttt attttgcaat atgggtttac tcacttgcca gccgacatta tttatggagc
     7201 agcccatgta aacaatattg catccaatcg cattttacgg aagaatggtt ttcaatttaa
     7261 aaataccttt ttctatgaaa gcgatttgtg taattggtat gcacttgaga agtcattgtt
     7321 cgaagggaat cttccttgat ggtatttgct tgattaagcc agaagattac atattgtaat
     7381 acttgttgtt agaggtagtt tgttttccta tatttgtcga aagcattttc ctcatgagca
     7441 ctcaaactgt gcggcttacc gccgaattca aaaagcaaac taccagagta gtaattgtaa
     7501 ttttattatt tgtattagtt tatctgatac ttatagcatt agctgtcgga ctaacggctg
     7561 cctcgatagc gggcggaata tttatcattc ttgcctcccc tgcatttatt acccttgttt
     7621 tgggagttgg tatggccagt ttaggttcct tgattttaat atttttattg aaatttttat
     7681 ttacttcaaa taagtatgac agatcacatt tgatagaagt cactcgtgct gaagagcctg
     7741 ctttattcga catgatcgat gatatcgttg agcaggtaaa aaccgctaaa cccaaaaagg
     7801 tatactggtc ggctgatgta aatgctgccg tgttttacga ttccagtttt tggagtatgt
     7861 tcctgcctat acgaaagaat cttattattg gcatcggact gttgaataca cttaccgctg
     7921 aagagttgaa agcggtgttg tcgcatgaat tcggacattt ctcgcaacga accatgaagg
     7981 tgggaagcta tgtgtacaat gtgaataaag taatttttaa tatgttgtac gacaacgatt
     8041 catttggaag catggcatcc agatggggaa gcgtgagtgg ttatatcgct atttttgtta
     8101 tgctggcttt tcatattata aatgggatac aatacctgct caggaaaatg tatgagctgg
     8161 taaataaaag ctatagcagc ctttccaggg aaatggaatt tcatgccgat gagattgcag
     8221 cgagtataac gggttacgag ccattaaaaa ctgcgctgat gcgattttca atatcctctg
     8281 catcttataa tgaagtgttg aattattata atgaaaaggc ctctcaaaat ataaaaagcg
     8341 aaaacatcta cgccgatcaa acacttgtct tgaaattttt agcggagata aataaaattc
     8401 gggttgaaaa tgatctgcca aatgttacag aagaggagca aaacagattt aacaaatcgc
     8461 gattggtaat taaagaccaa tgggctagtc acccgacaac gaaagacaga gtcacccgcc
     8521 tggaaaatac cggttttcat gcaagcggat tacagcatca acccgcaaaa accttgcttc
     8581 gtaatgcagg aaacacctat aaacaggtta cagccaattt attttccaat gtaacttaca
     8641 aaggaaacac ctcatttgaa tcaccggaaa cactcataac agcatataga gaaaagtttg
     8701 agtctaatgc tttcccggca atttttaatt catactacga cgacaaaaac attgaagtat
     8761 tcaatcccgg agaggtaaca ccttccacag aaacgatctt gatggaagaa ttgtattctg
     8821 acaaagctgt tgaaagtgta tatgcggaaa ttggtctggc gagtgatatc aatgtactaa
     8881 aagatattgg caatggattt actcaggtga agtcatttga ttttgacggt atccgttatt
     8941 cgcgaaaaaa ggcgctcacg ttagttgata atttgaagcc ggagctcgat aaacttacgg
     9001 ctgaaaacaa agcacatgat attcgtatct acgccacatt tatctctcta gaacaaaaat
     9061 taggattagc acctaaattg cagaccatgt atcaaacact gtttgattat gccgctgatt
     9121 tagaaaggaa attgcaaatt tattcgcatc tggcttctaa actcgaattc gtaagtgttc
     9181 aaacatcgca tgaagaaatt gatcgcagat tggcgcttgt aagaccgctt gaagataaat
     9241 tgaaaaccga aatcaataaa atattatctg atacttttta tcagtcggaa atcaccgaag
     9301 atatgatgaa aaatatgcag tcatttactt ccagacatat tgagtatttt ttgcttggta
     9361 attatgtcga agaaaatttg tctctactat ataaggcaat gaataattat cgataccttg
     9421 ttttgcgagc ttattttatt ttaaaaagaa atattctgct ttatcaggca gaattgcatc
     9481 agaaaaccgt tgcctaagtt gttgagtttg agcagtgtgt gtattaatca ctatcctaag
     9541 attgattttc ctcactccaa taacattgca ggatattgta actttgtata aaatccgttt
     9601 tatgaaaagc atgattacag gtgctgcggc gcttattttc ttcctcacta ctgcctttta
     9661 tcccggcgat aactgtgaat cttatttcgc taataaagtc ggcacatcta tggaacagac
     9721 cagttatgat gcgaagggga aaatgacttc taaatcagtt agcactgtga aggcctatga
     9781 cgctatagat ggtggctttg cctctacttt gaatgttcgt gttatggatg ccaaagacaa
     9841 agaagtatca aacagcgatg taatcatgaa atgccagcag gataaatttt atatggacct
     9901 cagcaatgca tttcctaaag aaatggccgg aatggaaggt gctgaagtgg aaatggaaaa
     9961 cacatttgtt gaatttcctg cgcatccggt agctggtcag tcacttcctg atcaaacaag
    10021 cagcatgacc attaaattaa atggcactcc atttatgaca atgaccatca acacgacgaa
    10081 tcgcaaggta gaaggatttg aatctatgac aacaactgca ggaacattcg agtgtatgaa
    10141 atattccgca gacactgaag taaaatctat gtttacctcc aaatcacatt cggtgatgtg
    10201 gatgtcgaaa aacgttggtc tcgtgaaaat ggagagctac gacgacaaag gaaaacttca
    10261 ggcaacacaa atgctgacga agttctccga gtgatattat gtcaaataat ttgatccttt
    10321 gactagtgct tatacatgac tcaaagcgcg ttcaatatcg aagataaggt cttcataata
    10381 ttctacgccg acacttaagc gaattaattt ttctgatata cctaatttct gtttgtcctc
    10441 aggcgataca tctgcgtgcg tcatcgtagc aggatgttca gcaagcgatt ctgttgaacc
    10501 tagtgaaacg gcaagtttga taagtttcag actattcaaa aaagtaaacg cttctttttc
    10561 acccccaata atatcaaaac ttaacatggc gccgggtgat gaatattgat tgcgataaat
    10621 gtgatattca ggtgttcctt ctggaattaa acctaaataa tacaccttct caaccttcgg
    10681 atgttcattt agaaattcaa caactttcat tgcattttgc gcttgcaatt ccatgcgcgg
    10741 ttttaatgtt tctaaactgc gcatcagcaa ccatccggta ttcggactca tcatatttcc
    10801 cataaatgtg cgcatggttt taatgcgcgt cattacttca tttccaccca aaacagctcc
    10861 agcaattaca tcgctgtgac ctccgatata ttttgtagcc gaatacatca cgatgtcagc
    10921 gccgtgcttc aaagggtggc tccacaatgg ccccatataa gtattatcaa ctgccaggta
    10981 aaccttttta tcgttgctat atacatcggc gatgcgcttg cacatttcca tatcgataag
    11041 cgcattggta gggttggcag gcgtttctac gtagatcagg ccaagggcat cagcgccttt
    11101 ttcctgaatg atggcatgta tctcctcctc agagctgttt gcatcaaagg cagcgtgtcc
    11161 gatgccccat tttggcaata catgtttaat gaagtgatca gttccaccat acaccggtgc
    11221 gctgaaaagc acatattgtc cgggttgcag gaattcgagg agcacggtag agatggcgct
    11281 catgccgctt gaaaacacag cacaagcttc ggcttcatcc caaagcgtaa gcctgttttc
    11341 gaggatctcg aggtcggggt tgttgagtcg cgagtagatg agaccttgtt cctcgccggg
    11401 ttccggactg ctgaggccat atgccacttc gaagaagcgt ttgccggctt cagcatttcg
    11461 gaatacgaag gtgctggttt gaaatatcgg gcatttcacg gccccctcac tcagctcggg
    11521 cttgtaacca aagctcatca tcagactctc ggggcgaaat ttatgttgtt ccattttaga
    11581 tgtggttgtg gcgtaaaact accattattc accacccaaa gaaatgaccc ccgtcaaccc
    11641 gaaacccccg aaaatcataa ctttcatccc tctgcaacaa acgctcccat atttgaactg
    11701 aaggcgacaa attcctgcaa tttttggaga aaaaatggac ggatgatgca aataaatatt
    11761 ttggggtgtg caggatgcgt atttgggtgt agtttgtgac agggcaaatg gggatgtttg
    11821 taaaatacgg cggcttgcga ctattgattt aattgcaatc tatgtgcaag gtttggttga
    11881 ttttgtgaca ttcggaattg ccggtctgac ttacattaaa ggaatttact ttagggtaaa
    11941 t
//
LOCUS       DAOQOU010000096        11940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2751_length_11940_cov_6.313504, whole genome shotgun sequence.
ACCESSION   DAOQOU010000096 DAOQOU010000000
VERSION     DAOQOU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2751_length_11940_cov_6.313504"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PLM90_09145"
     CDS             <1..1510
                     /locus_tag="PLM90_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89580.1"
                     /translation="AVSKINKISIQDTALYRNHPIFAMAQGDVLQAILHLPVPDTCFA
                     TWEEGDLVLACDRQQLLQMIDAVENKGTLTEQAEYQSFKGNVSASFNTSIFLHLASSE
                     QLIRQMVSDKQIDSIAKNFGLLQLFPRIELQFTEHKDMYMFNGFISYTATAERGAGML
                     WTTQIDAPVEAGPFAVMNDITGIRNILVQDTAHQLYMISGNGDVVWKRPLPSAILGEV
                     HEVDFYGNDKTQFLFNTGESIFLIDVRGDAVEGFPITLTTRISNPVALAMSGKSDYRM
                     FVACENKNVYGYYKDGKPIAGWNPLKSSGGINLPMFILPTSKGGVPGYIDAKSLAFFK
                     ANGSSLYTQKIPSPLMKEVMVMDSTVFMLDTMRTVFWVDSDFNGNNSKLASESTQCRL
                     LPGFADSSMGVVMLQNGFIKAAGLSGEPTVVSEPKGMVDQLVVLKGPDRWFYGYTTTT
                     GELALIDKQGQLLSGFPMPGSALSAISDLTLNGDAILITAVGSQLHAYRIHE"
     gene            1541..2485
                     /locus_tag="PLM90_09150"
     CDS             1541..2485
                     /locus_tag="PLM90_09150"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMZ89581.1"
                     /translation="MPITSTDFKLPGATAVYHGKVRDVYTISDNLLVMVASDRISAFD
                     VILPRPIPFKGQVLNQIAAYFLGATSDIAPNWLISTPDARVAIGHKCEPVKVEMVIRG
                     YLSGHAAREYKSGKRTICGVAMPEGMKENDKFPEPIITPTTKAAAGHDEDISREEILR
                     QGLVSEADYVQMEKYTRALFARGTEMAAKQGLILVDTKYEFGILNGEVVLMDEIHTPD
                     SSRYFYLEGYAGRQERGEHQKQLSKEFVREWLIENGFMGKEGQQVPEMSDAWIKTISD
                     RYIELYEKVTGRAFEPIDNSLDEINNNIVTELAKLNGR"
     gene            complement(2602..2674)
                     /locus_tag="PLM90_09155"
     tRNA            complement(2602..2674)
                     /locus_tag="PLM90_09155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2639..2641),aa:Arg,seq:tct)
     gene            complement(2736..3500)
                     /locus_tag="PLM90_09160"
     CDS             complement(2736..3500)
                     /locus_tag="PLM90_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89582.1"
                     /translation="MKKLCIAILLTAGCYQLGLSQAVGYQGKKFIVEAGFSPATNLLL
                     RYLDYSMVNDAIPEGERAPTEFKIIPRVSFEAVIFNSGSFFVRANPYNYSSTLSYLDK
                     NYDEVHLMQLDTKGFMFSIGYKSYNTPTPAPLGSYWGFNVTRYNFNSSYENSPDDSQG
                     LPNEISDYLIAESGCWGLFVSYGAKNIFWDKMTLDISLEGGYFFNNPEVGLTMDDNAF
                     GNDFEPIHLEYPQSINLTNTRAFFFIMPTIQLGYLF"
     gene            complement(3515..5830)
                     /locus_tag="PLM90_09165"
     CDS             complement(3515..5830)
                     /locus_tag="PLM90_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ89583.1"
                     /translation="MKILFPLLTLTVSTLSISAQSNDYQRVVNAGTLPSEVYTPSAEK
                     FKTTIEDLDSDLSKEEAKVQKQYYLESGFGIDEMMRSGTVLYNPEYNDYLNRIADELL
                     KNDPALRGDVRFYILRSPVVNAFAGAGGNIFISMGLIAMLDNEAELAFIMGHEIGHIA
                     KDHGLDYFVTASDIDKKSTDNALFKKSSSFDPTSIAKNMYSQALETEADDYGIDILRK
                     SKYWADTNALNAAFDVLKYAYLPYENEAFPISFFEGEHYKISNNLKLDSIKPITGTPE
                     LLTKKEALKSTHPSIGERRKTVNQKLGNLTNENRQAYIVSEADFRKLRNAARYELPMF
                     YLHNEFFQDAIYLSYLGLQKDPGNKYLEKIIGKSLTAYSKFRNSKEDEVYAEKARFED
                     YEGEQQQLYYLLWAINDAELNTMALLYNFKLHLKYPEDPEIIPQCQALVNDMVFYHFN
                     DEEDFLMGKNISTDSLYLLSDQANPKLKVAPPSSTSKVKTRKASVRSKRTTAAKVKID
                     KNKKQLLYAFNDYWENKDFRRLWNEAVVERDKREAEAKDLRKQGYKIKDTGDKRDYFF
                     GQKLGVEKVIVVNPFYKRIDLRKENAVEYVSSEEGELNYLEVLKTNAALVDIQLEILD
                     PLTLQATDVEKFNDMNELNDWFSEQLDFGSINLPGYNQAVIDSIATKYGTDYFLWTGI
                     VSLRDKQNLLGPILYIALSPAFIPLLPYGIYELVTPEYEFFYLSLLYDVKTHEGNVLK
                     FLFLKNNDSRAILNSHTYEMLNQIAAKPNNN"
     gene            complement(5923..8094)
                     /locus_tag="PLM90_09170"
     CDS             complement(5923..8094)
                     /locus_tag="PLM90_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMZ89584.1"
                     /translation="MRNMLVIAGMCTIVSACTNNKPQTEDDVKGPGKVTYPVTATADS
                     SSDFFGTTVADPYRWLEAEANDDPKVRQWVTDQNKVTFDYLAEIPYREQIKQRMLDLA
                     NYPKMTTPSRAGDYYYYQKNDGLQNQNVTYYRKGINGEEKVFLDPNKASADGTLTQSM
                     AGFNQKRTYVAINEAKAGSDWNTMYVKDVVNNTMMADKIEWTKFSGAAWQGDGFYYSA
                     YDKPSKTYTGSSENQKVYYHKIGDPQSKDKLIYTDPKHPLYYFSAQTTDDERYLFIYV
                     SPGTSGTEILWKDLRTNAKSFTKLFEGFDYEYTILDNDGDDLLVMTNYQAPNYRVVRV
                     DPTKPAPENWTEVIAEQKELLSGVSLAGGYLFASYLRDVKPVIIQFDRYGKPVRQIGL
                     PNNGVGGTVAGFNGEKDDKTVYFSFTTFLTPADIYSYDIESGKIELYKKAEVKFNSSD
                     YTIKQVFYTSQDGTKVPMFIVHKKGIKLDGSHPTLLYGYGGFNIPIQPSFSISRVALL
                     EQGFIYAVANIRGGSEYGEEWHKAAMFEKKQNVFDDFIAAAEYLIDQKYTSNTKLAIQ
                     GRSNGGLLVGACMTQRPDLYAVALPGVGVMDMLRYHRFTVGWGWAVEYGCADSSKEAF
                     DYLSKYSPLHNIKPGTKYPATMVTTADHDDRVVPAHSFKFAAALQAAQTGDAPTLIRI
                     DTQAGHGAGKPLAKQIEEDADWISFTMWNTGIKHMTITDKP"
     gene            complement(8162..10051)
                     /locus_tag="PLM90_09175"
     CDS             complement(8162..10051)
                     /locus_tag="PLM90_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HMZ89585.1"
                     /translation="MGQDTSNSKHTRNKHLHFVSALLEDITALEKMIADGLLESDIER
                     IGAEQEICIVNENFSPGDNSIDLLQKIQDPQFTTELAKYNIEINLTPRTCIEGGLIAM
                     ENELLEKFIVARNHASALNSHLVIAGILPTISRKEITLDYLTPKERYHMLSKKLRDLR
                     GRKFDLYLKGVDELHIRHDSIMFEACNTSFQVHLQISPAELVTSYNWSLAIAAPVLAI
                     SSNSPLLLGKELWSEIRIALFQQSIDTRHSIDEIREQRPRVTFGKDWIYNSITEVYKE
                     DISRFEVLINDDIEEHALQTLNENKVPQLRALRMHNGTIYRWNRICYGITENKPHMRM
                     ECRYIPAGPTVRDEMANAAFWIGLMKAKPENVKKIWEHFDFKDVKSNFFKAARAGMES
                     AFVWRGKTISAYDLIKNELLPLAHAGLQNCGMPEEEIYLYLGTIEKRLETRTGAQWQV
                     QNYRRLKKEMNDADALRALTESMYTLQLQNIPVCNWPDIAVAEAVASNIAKMHTVRQL
                     MSTDVLTVYPEDPVALVARIMEWNDINHIIVEDRKGHIHGVITSGHIMELKEKQTNLD
                     ILPVKQVMRTELHTTSPDAPIESALEIMQTHWISSLPVVSENKLMGIVTKNDMLRWMS
                     LRDKR"
     gene            10141..10839
                     /gene="ung"
                     /locus_tag="PLM90_09180"
     CDS             10141..10839
                     /gene="ung"
                     /locus_tag="PLM90_09180"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HMZ89586.1"
                     /translation="MSSPAGNKEIHIDPSWNNQLSNEFKQPYFAGIRDFLKKEKSAGK
                     TIFPPGPEIFNAYNLTPFEKVKIVIIGQDPYHGQGQAHGLCFSVQRGVAVPPSLGNIY
                     KEMRTDLGFDIPKHGFLESWAKQGVFMLNAILTVEKDQPASHKHIGWQFFTDATIRAL
                     NEHRDHLVFMLWGAFAQQKAALLDTKRHLVLTAAHPSPFSADKGFFGCKHFSKANEWL
                     KQHGEQQIDWKIPD"
     gene            10857..11249
                     /locus_tag="PLM90_09185"
     CDS             10857..11249
                     /locus_tag="PLM90_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89587.1"
                     /translation="MPLTDSDKQYISEMLTDALSACCPPMVLVSQSKTGMEIIGNVSV
                     PYGYSKKMIPGMYFSSFVVRKDMVSFYLFPLYGQVDKFEGLAPVAMKCLKGKTCFNFK
                     KKEQVISSEIKAMLKFGTEIWRKQGYVS"
     gene            11384..>11940
                     /locus_tag="PLM90_09190"
     CDS             11384..>11940
                     /locus_tag="PLM90_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89588.1"
                     /translation="MRTLLKWATFVLYLSASVPAFSQATEAWYIRRTPPEPWTWAPVL
                     NTNITEMDNVFGIGGWNSGYFTTVAVADAFGPDSKFVFLEGGDSHALDLKAFLDSNLP
                     TIENWVYNGGCLFLNAAPNEGADINFGFGGTTLDYTPGYFDGAVVATDATHPIFLGPY
                     TPVGTSWTGNYFCHAKVTGTCLTML"
BASE COUNT         3398 a         2449 c         2403 g         3690 t
ORIGIN      
        1 ggcagtttcg aaaataaata aaatcagtat acaggataca gcactatatc gcaatcatcc
       61 catttttgca atggcacaag gggatgtgtt gcaagccatc ctgcatttgc ccgttcccga
      121 tacttgcttt gcaacttggg aggaaggtga ccttgtgctt gcatgcgata ggcaacaatt
      181 actgcaaatg atcgatgcag ttgaaaataa aggcacattg actgagcaag cagaatatca
      241 atcatttaaa ggaaatgttt ccgcatcatt taatacctca atctttttac atctagcctc
      301 ttctgagcaa ctcatcagac aaatggtgag tgataaacaa attgattcaa tcgcaaaaaa
      361 tttcggattg ttgcaattgt ttccacgcat tgagttgcaa ttcactgagc ataaagacat
      421 gtatatgttc aatggattta tttcctatac ggcaactgct gaacgaggag caggaatgtt
      481 gtggactact caaattgacg caccagtgga ggcaggtcca tttgctgtga tgaatgatat
      541 taccggaata agaaatatcc tggttcaaga taccgcacat caattataca tgatctcagg
      601 aaatggtgat gtggtttgga aaagaccctt gccttctgca attcttggtg aagtacatga
      661 agttgatttt tatggaaatg ataaaacaca attcctgttt aataccggtg aatcgatatt
      721 tcttatcgat gtgcgcggtg atgcggtaga aggatttcca ataaccctga ctaccagaat
      781 atccaatcct gttgcacttg caatgtcagg aaaaagtgat taccgaatgt ttgtagcatg
      841 cgaaaataaa aatgtgtatg gctattataa agatggtaaa ccaatagcag gttggaaccc
      901 actgaaaagt agcggaggca ttaatctgcc aatgtttatt ttaccaacat caaagggcgg
      961 tgttccgggt tatattgatg caaaatcctt ggcatttttc aaagcaaatg gcagctcatt
     1021 gtatacgcag aagattcctt cacccttaat gaaagaagta atggtgatgg attcaactgt
     1081 tttcatgctg gataccatgc gcaccgtatt ttgggttgat agcgatttta acggtaataa
     1141 ttccaaactc gcttccgaaa gtacacaatg tcgcttgtta ccgggatttg ccgatagctc
     1201 catgggtgtg gtcatgctgc aaaatggatt tatcaaagct gccggtcttt caggtgaacc
     1261 taccgttgtt tctgaaccca agggcatggt agatcaatta gtagtattaa aagggccgga
     1321 cagatggttt tatggataca caaccactac cggagagctt gcactcattg acaagcaagg
     1381 gcaattgctt tcgggatttc ctatgccggg atctgcgctt tcagccatta gtgatcttac
     1441 cttgaatggt gatgcaatac ttattactgc agtcggttca caattacatg catatcgcat
     1501 tcatgaatga agtcgtattt ttgcaaagca ataatattgt atgccaataa cgagtactga
     1561 cttcaaatta cccggcgcta cagctgttta tcacggaaaa gtgcgggatg tttataccat
     1621 cagcgacaat ttactggtga tggtagccag cgacaggata tctgcgtttg atgtgatttt
     1681 accacgccct atacctttca aagggcaggt attgaatcag attgcagcat attttcttgg
     1741 cgctacgagc gatatagcac caaactggtt gatttctact cctgatgcac gtgtggcaat
     1801 agggcataaa tgtgaacctg tgaaagtgga aatggtaatt cgaggttatt taagcggtca
     1861 tgcagcacga gaatataaaa gtggaaaacg aactatttgt ggcgttgcta tgcctgaagg
     1921 aatgaaagaa aacgacaaat tccctgaacc aattattaca ccaacaacaa aagcggcagc
     1981 aggccacgat gaagatatat ccagagaaga aatccttcgc cagggattag taagtgaagc
     2041 tgattatgtt caaatggaaa aatataccag agcattgttt gctcgcggta cagaaatggc
     2101 agcaaaacaa gggttgatat tagtagatac caaatatgaa ttcgggatat tgaatggtga
     2161 ggttgtctta atggatgaaa ttcatacccc cgattcatct cgatattttt accttgaagg
     2221 ctatgccggc aggcaagaac gtggtgaaca tcaaaagcaa ctgtcaaaag agtttgttcg
     2281 cgaatggctg attgaaaatg gctttatggg aaaagaaggt caacaggttc ctgaaatgtc
     2341 ggatgcctgg atcaaaacta tttcagaccg gtatatagaa ttatacgaaa aggtaacagg
     2401 acgggcattt gaaccaatag ataacagcct ggatgaaatc aacaataata tcgttaccga
     2461 gctagccaaa ctcaatggac ggtgacattg gtttcgtagt ataaacctta ttatagcctt
     2521 gtaaagctga cagccgtaaa atgcatccgg cagatacgtg caagctgcaa tttcttaatt
     2581 ttcgacctga aagtggaagt gtgaccccga ctggattcga accagtgacc cactgcttag
     2641 aaggcagttg ctctatcccc tgagctacgg ggcccgaaaa agaagtccgc ccggagattc
     2701 caagcggact gcaaaattac gtaaaccttt gcgaatcaga ataaatatcc aagctgaata
     2761 gtgggcatta tgaaaaagaa ggccctggta tttgtcagat tgatagattg aggatattca
     2821 agatgaatgg gttcgaaatc gtttccaaag gcattatcat ccatagtcaa gccaacttca
     2881 gggttgttaa aaaagtaacc accttctaaa gaaatatcca atgtcatctt atcccaaaag
     2941 atatttttcg ccccataact gacaaaaagc ccccaacaac ctgattctgc tatcaggtaa
     3001 tcggaaattt catttggaag accttgggaa tcatcaggac tattttcgta tgacgaattg
     3061 aaattataac gggtaacatt aaatccccaa tatgaaccca gtggtgcagg tgttggtgtg
     3121 ttgtaggatt tatatcctat tgaaaacata aagcccttag tatcgagctg catgagatgc
     3181 acttcatcat agtttttgtc cagataggat agagtagaac tgtaattata tggatttgcc
     3241 cttacgaaga aactcccact attaaaaatt acagcctcaa agcttactcg gggaatgatt
     3301 ttaaattctg taggagcgcg ttcgccttcc ggaatggcgt catttaccat actgtaatca
     3361 aggtaacgta acagcaaatt ggttgccggt gaaaatcccg cttcaacaat gaattttttg
     3421 ccttgatacc ctacagcttg tgacaatcca agttgataac atccggctgt cagcaatatt
     3481 gcgatgcata attttttcat gtcagttcag gggtttagtt gttattgggt tttgcagcta
     3541 tctgattcag catctcgtaa gtatgcgaat ttaaaatggc acgggagtcg ttatttttca
     3601 gaaacaaaaa cttcaacaca ttgccttcat gtgtttttac atcatataat aaactcaaat
     3661 agaaaaattc atattcgggt gtcactagct cataaatacc ataaggcaat agtggaataa
     3721 atgccggtga taacgcaata tacagtatcg gacctagtaa attttgcttg tcacgcaagg
     3781 atacaattcc tgtccataag aaataatcgg ttccgtattt ggtggctatc gaatcaatta
     3841 ctgcctgatt atatcccggc aagttgatgc ttccgaaatc cagctgttca gaaaaccaat
     3901 cgttgagctc attcatatca ttgaattttt caacgtcggt ggcctgcaat gtaagcggat
     3961 caagaatttc taattgtata tccactaatg cagcatttgt tttcaaaact tccagataat
     4021 ttaattctcc ttcttcactg gatacatatt caacggcatt ttcttttcga agatctatgc
     4081 gtttgtaaaa cggatttaca acaattactt tttctacgcc aagtttttga ccaaaaaagt
     4141 aatcgcgctt atcgcctgta tctttgattt tatatccttg ttttcgcagg tctttagcct
     4201 ctgcttcgcg tttgtcgcgt tctactactg cctcgttcca gagtctcctg aaatctttat
     4261 tttcccagta atcattgaaa gcatataaca attgcttttt gtttttatcg attttaactt
     4321 tagcggcagt agtccgttta cttcttacag aagcttttcg tgttttcacc ttagaagttg
     4381 aacttggtgg tgcaactttt aactttggat tggcctggtc agataataaa taaaggctat
     4441 cggtgctgat gtttttaccc attaaaaaat cttcttcatc attgaaatga taaaagacca
     4501 tatcatttac caaggcctga cattgaggaa taatttcggg gtcttccgga tattttaaat
     4561 gcaatttgaa gttgtacaac aatgccatag tattcaattc ggcatcattt atggcccata
     4621 acaggtagta aagctgttgt tgttcgcctt cgtaatcttc aaaccgagct ttttcggcat
     4681 aaacttcatc ctccttactg tttctgaatt ttgaatatgc cgttaatgat tttccgatga
     4741 ttttttccaa atacttattg ccgggatcct tttgaagccc taggtaactc aaataaatgg
     4801 catcttgaaa aaactcattg tgcaaataaa acatcggtaa ttcgtaacga gcggcattgc
     4861 gcaattttct gaaatctgct tctgaaacaa tatatgcctg acggttctca tttgtgaggt
     4921 tacctaattt ttgattcaca gtttttctgc gctcgccaat acttgggtgt gtgcttttta
     4981 aggcttcttt ttttgttaat aattccggag tgcccgtaat tggttttatt gaatccaatt
     5041 tgagattatt gctgatcttg taatgttctc cttcaaaaaa actgattggg aaggcctcgt
     5101 tttcatatgg cagatatgca tatttcaaga catcgaaagc ggcattcaag gcatttgtgt
     5161 ctgcccagta tttagatttc cttaaaatat cgataccata atcatcagcc tctgtttcca
     5221 atgcttgtga atacatgttt ttagcaatag aagttgggtc gaaggaagat gactttttga
     5281 ataatgcatt gtcggtagat ttcttatcta tatcgcttgc cgtaacaaaa tagtctaatc
     5341 catgatcctt tgcaatgtga ccgatttcat gccccatgat aaaagccaat tcagcttcgt
     5401 tatccaacat ggcaataagt cccatgctga taaaaatatt tccaccggca ccggcgaatg
     5461 cattcactac cggtgaacga agtatgtaaa aacggacatc accacgtaat gcagggtcgt
     5521 ttttcagcaa ttcatctgca atgcggttga ggtagtcatt gtattcggga ttatacaaca
     5581 cagtcccgct gcgcatcatc tcatcaatgc cgaagccact ctcgagataa tattgctttt
     5641 gaaccttagc ttcctctttg ctgaggtcgc tgtcaaggtc ttcgatcgtt gtcttgaatt
     5701 tttcagctga aggtgtatac acttcagaag gaagggtgcc ggcatttact acgcgctgat
     5761 agtcattgct ttgtgccgaa attgacagag ttgacactgt caaagtcagc aaggggaaaa
     5821 gaattttcat gtatgagtta gttaggctgt atgcaagttt atgaaaaata tgggtaaaaa
     5881 aaaaggaggc tcaaggcctc cttatatgca aattatttca tgttatggtt tatcagttat
     5941 ggtcatatgc ttaattcccg tattccacat ggtgaagctt atccaatcgg catcttcttc
     6001 aatttgtttc gccagtggtt taccggctcc gtgaccggct tgtgtatcga ttcgaattaa
     6061 ggttggagca tccccggtct gggcagcctg tagtgccgca gcaaatttaa agctgtgcgc
     6121 aggaacaacc ctgtcatcat ggtctgctgt ggttaccata gtagccggat attttgtacc
     6181 cggtttgata ttatgtagag gtgagtattt tgataaataa tcaaatgcct ccttggaact
     6241 atcggcacat ccgtactcaa cagcccatcc ccagcccaca gtaaagcgat ggtaacggag
     6301 catatccatt acacctactc ctggtaaagc aacagcatag agatcaggac gctgggtcat
     6361 acatgcacca acaagcaaac caccgttcga tctaccttga atggcaagct ttgtgttgga
     6421 tgtatatttc tgatcaatca agtattcggc agcagcaatg aaatcgtcaa atacattttg
     6481 cttcttttca aacattgcag ctttatgcca ctcttcgcca tattcacttc ctcctctgat
     6541 attggctacg gcataaataa atccttgctc caacaatgcc actctgctga tactgaaaga
     6601 tggttggata ggaatattaa atccaccata tccataaagt aatgtagggt gactgccatc
     6661 gagtttaata ccttttttgt gtacaataaa catcggtact ttagtgccat cctgactagt
     6721 ataaaacact tgtttaatgg tatagtcgct gctgttgaat ttcacttcag ctttcttata
     6781 taattcaatt ttacctgatt caatatcgta ggaataaata tcagctggtg taaggaatgt
     6841 ggtgaaagaa aaataaactg ttttgtcatc tttctctccg ttaaatcctg ccacagttcc
     6901 acctacacca ttattaggta agccaatctg acgaacaggc ttgccatagc ggtcaaattg
     6961 aatgatcacc ggtttcacat ctcgtaaata agaagcaaac aaataacctc ctgccagaga
     7021 aacgcctgaa agtaattctt tttgttcagc aattacctca gtccaatttt caggtgccgg
     7081 ttttgttggg tctacacgaa caactctgta attgggtgcc tggtaatttg tcatcaccaa
     7141 taaatcatcg ccatcattat caagaatggt gtactcataa tcaaagcctt caaacaactt
     7201 ggtaaatgat tttgcatttg tgcgcaaatc cttccataat atttcagttc ccgatgtgcc
     7261 cggactcaca taaataaaca gataacgttc gtcgtctgta gtctgtgcac tgaaataata
     7321 taatggatgt ttaggatctg tataaatcaa tttatccttt gattgcggat cgccgatttt
     7381 atggtagtat actttttgat tttcactgct gccggtataa gtttttgatg gcttgtcgta
     7441 agcactgtaa taaaatccat caccttgcca agctgcaccg ctgaattttg tccattcaat
     7501 tttatcggcc atcatggtat tattaaccac atcttttaca tacatcgtat tccaatcact
     7561 tcccgccttg gcctcattta ttgctacgta agtacgcttc tggttgaatc cggccatcga
     7621 ttgggttaaa gtgccatcgg cagatgcctt gtttgggtca agaaaaactt tttcttcgcc
     7681 atttattccc ttgcgatagt aagttacgtt ttggttttgt aagccatcgt tcttttgata
     7741 gtaataataa tcaccggcac gacttggtgt tgtcattttg ggataattgg cgaggtctag
     7801 catgcgctgt ttgatttgtt cgcgataagg tatttcagca agatagtcga aggttacctt
     7861 attttgatct gtcacccatt gacgcacttt cggatcatca ttggcttcag cttctagcca
     7921 acggtaaggg tctgctaccg ttgttccaaa aaaatctgag gaggaatccg cggttgcagt
     7981 caccggataa gttactttac ccggtccttt tacgtcatct tccgtctgag gtttgttgtt
     8041 tgtgcaggca gacactatag tgcacattcc tgcgattaca agcatatttc gcatatggtt
     8101 ttcaataata taggagtaaa attactcaaa ttgccggcag tatgaaccct gctaacagta
     8161 tttaacgctt gtctcgaagc gacatccatc tgagcatatc atttttggtg actataccca
     8221 tcagtttgtt ttccgataca accggcaaac tgcttatcca atgggtttgc atgatttcca
     8281 acgctgattc tattggggcg tccgggctgg tagtatgcag ctctgttcgc atcacttgtt
     8341 taacaggtaa tatgtcgaga ttcgtttgtt tttctttcaa ctccataata tgccctgaag
     8401 taatcacacc atgaatatgt cctttgcgat cttctactat gatgtgattg atgtcattcc
     8461 actccataat tcttgcaacc agggcaacag gatcttcagg atatacagtc aacacatcag
     8521 tgctcatcaa ctgtcttact gtatgcattt ttgcaatatt agatgccact gcttccgcta
     8581 cagcgatatc aggccagttg caaacaggaa tgttttgtaa ttgtaaggta tacatgcttt
     8641 cagtcagcgc gcgcaaagca tctgcatcat tcatttcctt tttcagacgg cggtaatttt
     8701 gcacttgcca ttgtgcgccg gttcttgtct caagtctttt ttcgatggtg cctagatata
     8761 aataaatttc ttcctccggc ataccgcagt tttgcaatcc tgcatgtgcc aatggcaata
     8821 attcattttt aatcaggtca tatgcagaaa tagttttccc tcgccataca aatgctgatt
     8881 ccatgccagc acgtgctgct ttaaagaagt tggattttac atctttaaaa tcaaaatgct
     8941 cccatatttt ttttacattt tcaggtttgg ctttcatcaa cccaatccaa aatgctgcat
     9001 ttgccatttc atcgcgaacg gtcggacctg caggaatgta gcgacattcc atgcgcatat
     9061 gcggtttgtt ttcggtaata ccataacata ttctattcca ccggtagata gttccgttgt
     9121 gcatacgcaa ggcccgtaat tgaggcacct tattttcatt taacgtttga agcgcatgct
     9181 cctcaatatc atcgtttatc aatacttcaa aacgactgat gtcttcttta tatacttctg
     9241 ttatagaatt atatatccag tctttaccaa aggtaacgcg aggtcgctgc tcacggattt
     9301 catctatact gtgacgtgta tcaatgctct gttgaaataa tgctattcga atttcactcc
     9361 acaattcttt tcctaataac aaaggtgaat ttgaactgat tgctaacaca ggcgctgcaa
     9421 tggctaaaga ccagttgtaa gatgtcacta attcggcggg agaaatctgc aagtgcacct
     9481 gaaaactggt attacatgcc tcaaacataa tactgtcgtg gcgaatatgt aattcatcaa
     9541 caccttttaa atacaaatcg aatttgcgac ctctcagatc ccgcaatttt ttactgagca
     9601 tatgataacg ctcctttggt gtgagataat caagtgttat ttccttgcga cttatagttg
     9661 gcaatattcc ggcaattact aaatgactgt ttaatgcgga ggcgtgattt ctggcaacaa
     9721 tgaatttttc caacaactca ttttccatgg caattaatcc gccttcaatg catgtccggg
     9781 gagttaaatt gatttcgata ttatattttg ccagttcagt ggtgaattgt ggatcctgta
     9841 ttttttgaag taaatcgata gaattatcgc cgggagaaaa attttcatta acaatacaaa
     9901 tctcttgttc ggctccaatg cgctctatgt cgctttccaa aagtccgtct gctatcattt
     9961 tctctaacgc agtgatatcc tcaagcaaag cactgacaaa atgcaggtgc ttattccgcg
    10021 tatgcttact attacttgta tcctgtccca tggagtaatc caatcaaatt tatcacaaac
    10081 ctatggggtt gttggtgata ttcatcattt ctgcttaaaa ttgttgtcta cctgaccact
    10141 atgtcttcac ctgccggcaa taaagaaata catatcgatc catcgtggaa caatcaattg
    10201 agcaatgagt tcaagcagcc atattttgct gggattcgcg actttttgaa gaaagaaaaa
    10261 tcggcgggta agaccatttt tcctccgggt cctgagattt ttaatgccta taacctgaca
    10321 ccttttgaaa aggtcaaaat tgtaattatt ggacaagatc catatcacgg ccagggacag
    10381 gcacacgggc tgtgtttcag tgttcaaaga ggtgttgccg tgccgccatc attaggcaat
    10441 atctataaag aaatgcgaac tgaccttgga ttcgatattc ccaaacacgg cttcctcgaa
    10501 tcatgggcaa aacaaggcgt ttttatgttg aatgcaatat tgacggtaga aaaagatcag
    10561 cccgcatcgc ataaacatat tggttggcaa ttttttaccg atgcaacaat cagggctttg
    10621 aacgagcata gagaccattt ggtttttatg ctttggggcg cttttgctca acaaaaagct
    10681 gcattgcttg ataccaaaag acacctggtt ctgactgcgg cacatccttc gccgttcagt
    10741 gccgacaaag gtttcttcgg ttgtaagcat ttttcaaaag caaatgaatg gctgaaacaa
    10801 cacggcgaac agcaaattga ctggaaaatt cctgattaac acttaacaat acatgtatgc
    10861 cactaactga tagcgataag caatatatat ctgagatgct tacagatgcc ttaagcgcct
    10921 gctgccctcc catggttttg gtttcacaaa gtaaaaccgg aatggaaatt atcggcaatg
    10981 tttcagtccc ttatggctac tccaaaaaaa tgattccggg catgtatttt tcatcttttg
    11041 tggttcgaaa agacatggtt agtttttatc tctttcctct atacggtcag gtagataaat
    11101 ttgaaggtct ggcacccgtg gccatgaagt gcttaaaagg caaaacatgc ttcaacttca
    11161 aaaaaaagga gcaggtaatt tcttcagaga tcaaggccat gctcaaattc ggcacggaaa
    11221 tttggcggaa gcaaggctat gtttcataag gtttaagcgc ttattgcact gaaaatgatg
    11281 gttttgcacc atttttttgc ctatttgctt tgagagttca aagactattt ctagtttagg
    11341 attacggttt aagcgattat aaccgcttag acccacctaa cacatgcgca cactcctaaa
    11401 atgggcaaca tttgtcttat acctgtctgc ttcggtaccg gcattttcgc aagctacaga
    11461 agcctggtac attcgcagaa cccctcctga accgtggaca tgggctccgg tcctcaatac
    11521 caatatcaca gaaatggata acgtttttgg tatcggcggc tggaacagcg gttactttac
    11581 cacagttgcc gttgctgatg cctttggtcc ggattctaaa tttgtgttcc tcgaaggcgg
    11641 cgatagtcat gcactggact tgaaagcctt cctcgatagt aacctgccta ctatagaaaa
    11701 ctgggtttac aacggcggtt gcctgttcct gaatgctgca ccaaatgagg gtgctgatat
    11761 aaactttggt tttggcggaa caacactgga ttatacaccc ggatattttg acggcgcagt
    11821 tgttgcaact gatgctactc atccaatttt cctcggacct tatacaccgg tgggaacctc
    11881 atggacaggt aactatttct gtcacgctaa agtgaccgga acttgtctta ccatgttgat
//
LOCUS       DAOQOU010000097        11818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2786_length_11818_cov_4.994644, whole genome shotgun sequence.
ACCESSION   DAOQOU010000097 DAOQOU010000000
VERSION     DAOQOU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_11818_cov_4.994644"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLM90_09195"
     CDS             complement(<1..198)
                     /locus_tag="PLM90_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMZ89589.1"
                     /translation="MSRKKIVAGNWKMYKTLPEAMETIGTLTHALAVQSPKENVEVWI
                     APPSVILPMIIHNYHNRGISFG"
     gene            complement(206..1414)
                     /locus_tag="PLM90_09200"
     CDS             complement(206..1414)
                     /locus_tag="PLM90_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HMZ89590.1"
                     /translation="MNIILFDDKRWQHLLPLTFTKPVAGIRIGIDTIAEKWNSSVNGT
                     YSFLAEPYLQGKFPVRYGDDNLYIYGGLIPDAQIIAAITSLKTNDVLISGEDILAVRS
                     GERYFSGMSLNEHAAGLNKMHYNGSCICISRPYHIFQHNDVVLKADFARLTAGKKSAE
                     IPAGNTIIGDHIFIEEGAVIHASIINTTTGPVYIGKGAEIMEGSMIRGPFALGEHSVV
                     KMGAKIYGATTIGPWCKVGGEINNSVFMAYSNKAHDGFVGNSVIGEWCNLGADSNCSN
                     LKNTYAEVKLWDYSLQKFASTGLQFCGLIMGDHSKCGINTMFNTGTVVGVHANIFGDG
                     FPRNFIPSFSWGGAAGFVTYKLKDALTTTKTVMSRRELNLSSEDIKILTTIYELSEKY
                     RKGQLIFLHK"
     gene            complement(1490..1738)
                     /locus_tag="PLM90_09205"
     CDS             complement(1490..1738)
                     /locus_tag="PLM90_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HMZ89591.1"
                     /translation="MKKGIHPENYRPVVFKDFSVDFAWLGMSTANTKETIKWEDGNEY
                     PLIKLEISNASHPFFTGKMKFVDTAGRIEKFNKKYAKK"
     gene            1840..2310
                     /locus_tag="PLM90_09210"
     CDS             1840..2310
                     /locus_tag="PLM90_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HMZ89592.1"
                     /translation="MHWRTFNGRTIDRPIEEEVRETIERETAAGNKLKVCIGSDSQVY
                     GPIIEFGTVIVFLREGRGGFMYIANETSKQRMSLKERLLHEVSRSIQVAYGLCNLLDE
                     YHVDLEVHADINTNPHFQSNTALQEAMGYILSMGFVFRAKPDSFASSSCANKVV"
     gene            complement(2307..2906)
                     /gene="yihA"
                     /locus_tag="PLM90_09215"
     CDS             complement(2307..2906)
                     /gene="yihA"
                     /locus_tag="PLM90_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMZ89593.1"
                     /translation="MLIKTATYEGSFVETNKCPAGGLREFAFIGRSNVGKSSLINMLT
                     GRKELAKTSVKPGKTQTINFFLINEQWHLVDLPGYGYARVSRTLRGTWESMISDYLQK
                     REQLQCVFQLIDGGIPPQKIDLEFTNWLGENGIPFAIIFTKTDKRHKGEGDTPGAYSK
                     EMLKTWENLPPMFITSSTKRIGRDEVLDYLDFILRMPTL"
     gene            2987..3718
                     /gene="ubiE"
                     /locus_tag="PLM90_09220"
     CDS             2987..3718
                     /gene="ubiE"
                     /locus_tag="PLM90_09220"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HMZ89594.1"
                     /translation="MSEPVTPYHTKASKKEEVRNMFDNIAGRYDLLNRVLSLRIDVYW
                     RNKAVAFLKPFKPAHILDVACGTGDFSIAALKTGAQKVTGVDISEKMLAAGRVKMKER
                     GLDGKIALETGDSEQLQYATATFDAATVAFGVRNFEQLDKGLAEMQRVLKPGAPIVIL
                     EFSKPRVFPVKQLYHFYFSTILPLIGRLVSGDARAYTYLFESVSAFPDGNDFLDHMNK
                     AGFTSCAAKRLTFGICSMYTGLKKD"
     gene            3685..4392
                     /locus_tag="PLM90_09225"
     CDS             3685..4392
                     /locus_tag="PLM90_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ89595.1"
                     /translation="MFNVHRFKKGLITLLMLVPFLSKAQMNVTEYENRFSIRPYHFGI
                     SLGYNSTDFRIAFADQFIGHDSVMQVLSTPGPGFNLGILTNLRMGKYFDLRFIPTLSF
                     AEKNLNYTYRNESTSTQTVESLYLCMPFDVKFKSAAYKDMKVYVLGGMQYTYDFSSNA
                     EARNAEGLVKIKPNDIAVNAGFGFEFYFPYFIFCPEIKLSNGIVNIHAVDPLLQESYV
                     MDKLLSRTLLFCIHIEG"
     gene            4454..6610
                     /locus_tag="PLM90_09230"
     CDS             4454..6610
                     /locus_tag="PLM90_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HMZ89596.1"
                     /translation="MKRVFLICLFFIPAILFAQSTISVSDLWVNYRFYPAGIDDLRSM
                     QDGEHYTVNEKGEAIVRYAYADGKKSGDVFNVNQTGGAVTQFQDYHFNADETSILLTT
                     ASERRYRWSSIDENYIYNTKTQQTIKLSDKGGQMYADFSPKQNRIAYVIDNNLYYKDL
                     DNNREYAITTDGKWNEIINGGSDWVYEEEFTQVRSFAWSPDGNKIAYYKFDESEVPEF
                     DMMTYKGQLYPKNYEYKYPKVGEKNSKVVIYIYDIATQKSTVVNIAKPYEYIPRIKWT
                     NDGSALCVFTMNRLQNDLELFLADPATGNTRSMFHETAKYYLEINDDLTFLKDNKSFI
                     WRSEQDGWNHLYRYDMNGKLINQITSGKFEVTQVLGINEEKGVVYYMSTEESPYERHL
                     YATQLDGKKTKKITSRKGNNSVNFSTGFKYFINTNSTFMQPDYITLCDGSGKEIRVME
                     DNAALQKDVDQLDIAAPQFMQIPGADGNGLNAYMIKPKNFDASKKYPVLMTVYGGPGS
                     QEVIDGFDGFNMMWHQMLAQNGYIIVCVDNRGTGARGRDFRQVTYGQLGKFETDDQIA
                     AAQWLGKQTYIDASRIGIWGWSYGGYMAGLCITRGADVFKTAISVAPVTNWKYYDNIY
                     TERYMGTLETNPTGFDANAPMAYADMLKGNYLLVHGTGDDNVHFQNSVEWINALVRNN
                     KQFDLMVYPDRNHGIYGGKTRLHLYTLMTDYILENL"
     gene            complement(6615..7400)
                     /gene="folP"
                     /locus_tag="PLM90_09235"
     CDS             complement(6615..7400)
                     /gene="folP"
                     /locus_tag="PLM90_09235"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMZ89597.1"
                     /translation="MGILNVTPDSFYDGGRFVEGLSAVEQAERMVKAGVDIIDIGGMS
                     TRPGADIISPREEMDRVLPVIAEIRVEFPEMLISIDTVYGRTAREALKAGADIINDVS
                     AGTMDSDIFQAAIDHQAPYILMHMQGNPQTMQLEPYYEDVVEEVLTFLSEKTTSLKHH
                     GLQQVILDPGFGFGKTLTHNYQLLASFEKFLALGCPVLAGVSRKSMICKLLHISPDHA
                     LNGSTALHALLLQKGAHILRVHDVKEAAQTRAIFMAMQNPLGL"
     gene            7487..8059
                     /locus_tag="PLM90_09240"
     CDS             7487..8059
                     /locus_tag="PLM90_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HMZ89598.1"
                     /translation="MSTKLMQSDVSKTNLQYDDVVEGCRNIYLKKIKDYGTSWRILRP
                     TSLTDQIFIKAQRIRTLEQTGVSRVGEGIHDEFRGIINYAIIALIQLEMGESGPVNLS
                     EEKALELYNEKIGKVKKLMLDKNHDYGEAWRDMRISSFTDLILMKLLRVKQIEENDGK
                     TLISEGVDSHYSDMINYSVFALIKLSEDEK"
     gene            8049..9728
                     /locus_tag="PLM90_09245"
     CDS             8049..9728
                     /locus_tag="PLM90_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HMZ89599.1"
                     /translation="MKNKLLTICRILTGGLFIFSGLVKANDPVGFGIKLEEYYDVFAG
                     RWSWTAWLFHSDLMLNTVNLQAAFLTTLEVALGIALLMGLWKNLVSWLLLLLILFFTW
                     LTGYSAITGSVTDCGCFGDFIKLTPLQSFYKDLVLLALILVIFFLRKGLKPIKPLGIS
                     IGLFLTVTVFAIWVNVHVLQHDVFLDWRPYAVGDNITENMKLPENAQPDITEMIYAYT
                     NKKSGEQVTLKYLNTELKEKKNLDELLSYSNNKDTWKLDTSYTKIKVKGDRAKIIDFA
                     ISDEHDEFITDQILGNPDYALMVVSAGFEHTNKEGWDRISAVQKDAEKEGLFTFALVG
                     EGRTEIEKFRHNQQMAFPFYTADYKVCLTIARVNPNVVLMKEGTIIAKWAWRDLPSFE
                     EIKKTYFPERKPTELMVISNEMFAPGIQVSAKLRDAEEPYNEFFLQDTVGNDLTTALI
                     NDTTNAVMFIINELSSDKLTMENWKSILAVMQMSNTAQKPFFVASSGSPEVLEMMKKA
                     SGLEFGYYVSDKDVLAKMMETNTGLIVIKNGKVTAKYADGKLPSDSNFLTQ"
     gene            9738..10814
                     /locus_tag="PLM90_09250"
     CDS             9738..10814
                     /locus_tag="PLM90_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ89600.1"
                     /translation="MISYIIKRLGYGVLVLIGVVIVVFFLFQLTRIHPERQIAGEKAD
                     KETIENIKKELGLDLPVSQQLLLYLNDISPLSFHQVADSSSRYFLDGGKYDYFRLFTI
                     NHTATVLKKPYLSRSYVSKRSVNEILSQRIPNTLVLALAAMLLATVVGIALGVIAAVK
                     QGTAIDRLFTAGSVLGISFPSFFAGIILQLIFAYLLASITGFNMTGNLYETDPYTGER
                     YLALQNLILPAIALGIRPIAVITQLTRSAMIDVLSNDYIRTARAKGLSERKIVFRHAL
                     KNALNPVVTSISGWLAGLLTGAFFIEAIFGYNGIGYETVNALKTFDYPVAMGAILYTA
                     VIFVVINIIVDILYSVIDPRIRIA"
     gene            10811..11323
                     /locus_tag="PLM90_09255"
     CDS             10811..11323
                     /locus_tag="PLM90_09255"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMZ89601.1"
                     /translation="MRVYLIGFMGCGKTTFGKKLARKMNVDFIDLDKQIEAHEGKSVS
                     EVFAEKGEAYFRELESSLLQQTISLNHAVISCGGGTPCFHDNMHWMREHGITVYLKVT
                     PEFLFSRLHTRREKRPLIAKLNDEELQAYITTKLEEREVHYQQAQQVIDPVHAKPGFV
                     ADLLNARKGN"
     gene            complement(11330..>11818)
                     /locus_tag="PLM90_09260"
     CDS             complement(11330..>11818)
                     /locus_tag="PLM90_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMZ89602.1"
                     /translation="SPEKVLQKIKRIAFEIAEDNYDEPEIVLAGICKETEGYAFAELI
                     YQELKKISSAHIILTHVELDKHNPGDSVVELNLTEEQIDNKVIILADDVSNTGRTLLY
                     AIKPLLSYSPKKIRSVVLVERKHKLFPVTPDYVGLSLSTTIHEHIKVVLSDKQHAGVY
                     LM"
BASE COUNT         3430 a         2354 c         2600 g         3434 t
ORIGIN      
        1 accaaaactg attccacgat tgtgataatt gtgaataatc ataggtaata tcactgaagg
       61 aggagctatc caaacttcta cattttcttt tggcgattgc actgctaaag catgtgtcag
      121 tgtaccaatg gtttccatcg cttcaggaag tgttttgtac atcttccaat tccctgcaac
      181 aattttcttt ctgctcatag ttgtattatt tatgcaggaa tatcaattga cctttcctgt
      241 atttttcact taattcataa atggtggtga ggattttaat gtcctctgat gataaattta
      301 attcgcgtcg tgacatcaca gtttttgttg tggttagtgc atccttcagc ttataggtga
      361 cgaatcctgc tgcaccgccc cagctgaaag aaggaatgaa attgcgcgga aagccatcgc
      421 cgaaaatatt ggcatgcaca cctacaacgg ttccggtatt gaacatggtg ttgatgccgc
      481 acttgctgtg atcgcccatg attaaaccgc aaaattgtaa tcctgttgag gcaaattttt
      541 gaagcgaata atcccataat ttcacttcgg cataggtatt tttcaggttg gaacaattgc
      601 tgtcggcacc tagattacac cattcgccaa taacactgtt tcctacaaag ccgtcatgcg
      661 ctttattgga atatgccatg aatacactat tgttgatctc gccaccaacc ttacaccatg
      721 gacctatggt tgtggcgcca taaattttcg cacccatttt tactacgcta tgttcaccta
      781 atgcgaatgg tcctctgatc attgaacctt ccattatttc agcgcctttt ccaatgtaaa
      841 caggtcctgt tgtcgtgttt atgattgatg catgaatgac ggccccttcc tcaatgaaaa
      901 tatgatcgcc aataatggta ttgcctgccg gaatttctgc actctttttt cctgccgtta
      961 agcgagcaaa atcagccttt aatactacgt cgttatgttg aaatatgtga tatggtcttg
     1021 aaatacaaat gcaacttccg ttgtaatgca ttttatttaa tcctgcagcg tgttcattca
     1081 gcgacatacc cgaaaaatag cgctcaccgc ttcgcaccgc taatatgtct tcacctgaaa
     1141 ttaatacgtc gttggttttt aatgatgtga tggctgcaat aatttgtgca tcaggaatca
     1201 gaccaccata aatgtacaga ttgtcatcgc cataacgaac cggaaatttg ccttgcagat
     1261 aaggttccgc taaaaatgaa tatgtaccat ttacggagga attccatttt tcggcaatag
     1321 tatctatgcc gatgcgaata ccggctacag gttttgtaaa agtgagtggt aacaaatgct
     1381 gccaacgctt atcatcgaag agtataatgt tcatgatgcg acaaatttaa atgaaaaacc
     1441 ccttctgcac acaaggtacg gaaggggttt aataggtttt agccgattat tatttcttgg
     1501 cgtatttttt attgaatttc tcgatacgac ccgctgtatc aacaaacttc atcttaccgg
     1561 tgaagaatgg atggctggcg ttgctgatct ctaatttgat cagggggtat tcattaccgt
     1621 cttcccattt gatggtttct ttggtgttag cagttgacat acctaaccag gcaaagtcta
     1681 cgctgaagtc tttaaatact accggacgat aattttcggg atgaatgcct tttttcataa
     1741 ctactgttaa ttgaaggtgc aaaggtactg cttttttttc attcccggaa attatttccc
     1801 gcaatttatg cgtatacttg tagtatgacg ggcacaacca tgcactggag aacttttaac
     1861 ggcaggacca tcgaccggcc gatagaggag gaggtgcgtg aaactattga gcgggaaacg
     1921 gctgcaggca ataaactgaa ggtctgcatc ggttccgact cgcaggttta tgggcctatc
     1981 atagagttcg gcaccgttat cgtgtttctg cgcgaaggca gaggcgggtt tatgtatatc
     2041 gccaatgaaa cctccaaaca acgtatgagc ctgaaagaga gactgttaca tgaggtttca
     2101 aggtcaattc aggtggctta cggactatgc aatctactcg acgaatacca cgttgatctg
     2161 gaggtgcatg ccgacatcaa tacgaatccg cattttcagt ccaataccgc cctccaggaa
     2221 gccatgggtt atatcctcag catgggcttt gttttcaggg cgaaacctga ttcttttgcc
     2281 tccagcagtt gtgccaataa ggtggtctaa agggttggca tcctcagtat aaagtccaga
     2341 taatcaagca cttcgtcgcg gccaatgcgc tttgttgaag aggttatgaa catgggaggc
     2401 aggttttccc aggttttaag catttccttg ctatacgcac cgggggtatc gccttcgcct
     2461 ttgtggcgtt tgtctgtctt tgtgaaaatg attgcaaagg gtatgccatt ttccccaagc
     2521 cagtttgtga attctaagtc tatcttctga ggtgggatgc cgccatcaat gagctgaaac
     2581 acgcattgca gctgctcccg cttctgcaaa taatcgctga tcatgctctc ccaggtgccc
     2641 ctcaaagtcc tcgaaacgcg ggcatagccg tatccgggaa ggtctacgag atgccattgc
     2701 tcattgatga gaaaaaaatt gatggtctgg gtttttccgg gcttcacact cgttttggcg
     2761 agctctttgc gaccggtaag catattgatg agcgatgatt tcccaacgtt agagcggccg
     2821 ataaaagcga attcccgcag tccgccggca ggacatttat tcgtttcaac aaaacttcct
     2881 tcgtatgtag ctgttttaat aagcacgcgg caaaggtacg gtttcaaaca cgaattgaca
     2941 tcctgatacc tgcatgccga tattttacta ctacctttgc acccgcatgt cggaaccggt
     3001 aactccatat cataccaaag cctccaaaaa ggaggaagtg cgcaatatgt tcgacaatat
     3061 tgccggtcgc tatgacttgt tgaacagggt attgtcgctg cgtatcgacg tttattggag
     3121 gaataaggct gttgcatttc tcaaaccctt taaaccggct catattcttg atgtagcatg
     3181 tggaaccggt gatttttcta tcgcggcttt gaaaaccggt gcacagaagg ttacaggcgt
     3241 tgatatctca gaaaaaatgc tcgccgccgg aagggtgaaa atgaaagagc gtggacttga
     3301 tggcaagatt gctcttgaaa cgggagattc tgagcaattg caatatgcta cagccacctt
     3361 cgatgccgca accgttgcgt ttggtgttcg aaattttgag caactggata agggtcttgc
     3421 agagatgcag cgcgtgctta aacccggagc accaattgtg atcctggaat tctccaaacc
     3481 gcgtgtgttt cccgttaaac aactctatca cttctatttc agcacaatac ttccgctaat
     3541 aggtcgttta gttagcggag acgcaagggc atacacctat ttgttcgaat cagtgtcggc
     3601 attccccgat gggaacgatt ttctcgatca catgaataaa gcaggattta cctcatgcgc
     3661 tgcgaaaagg ctaacgttcg gaatatgttc aatgtacacc ggtttaaaaa aggattgatc
     3721 acattgctga tgctcgtgcc attcctgtcg aaagcacaga tgaatgtcac cgaatatgag
     3781 aatcgcttct ccatcaggcc ctatcatttt ggtatttcat tgggttataa ttctacagac
     3841 ttcagaatag cttttgcaga ccagtttatt ggtcatgatt cagtaatgca ggtgctttcc
     3901 actcccggtc ccggctttaa cctcggaatt ttaacaaatc tgcgcatggg taaatatttc
     3961 gaccttcgat ttattccgac cctgagcttc gctgaaaaaa atcttaacta cacttatcgc
     4021 aatgagtcta cctctacgca aacggtggaa tcgctatatc tgtgcatgcc ttttgatgtg
     4081 aaatttaaaa gtgctgcata taaggatatg aaagtgtatg tacttggagg catgcagtat
     4141 acctacgatt tcagctccaa tgccgaagcc cgaaacgcgg aaggacttgt taaaatcaaa
     4201 cccaatgata ttgcggtcaa tgccggattt ggatttgagt tttacttccc atatttcata
     4261 ttctgccccg aaataaaact cagtaatggc atcgtaaaca tccatgccgt agatccgctg
     4321 ctgcaggaaa gctatgtgat ggataaatta cttagcagaa cgctcttgtt ctgcatacat
     4381 atcgaaggct gatggttgct gcatagtatc tgaatgacaa ttctttacct ttacagataa
     4441 aataaacatc attatgaaga gagtattcct catctgtctg ttttttattc cggcaatctt
     4501 attcgcccaa tctactattt cggtatccga cctttgggtg aattatcgat tttatcccgc
     4561 cggtattgac gacctccgca gcatgcaaga cggcgaacat tataccgtga atgaaaaagg
     4621 tgaagctatt gtgcgttatg cttatgctga tgggaagaag agtggtgacg tctttaacgt
     4681 aaaccagaca ggaggcgccg taactcagtt tcaggattat catttcaacg cagatgaaac
     4741 ctccattttg ctcactacag catcggagcg cagatatcgc tggtcgtcga ttgatgagaa
     4801 ttatatttat aataccaaaa cgcagcaaac gataaaatta tctgacaagg gcggtcagat
     4861 gtatgccgat ttttcgccaa agcaaaatcg catagcgtat gtaattgata ataatttata
     4921 ttataaggat ctggataata atcgcgaata cgctattacc actgatggca aatggaatga
     4981 aatcatcaac ggtggcagcg attgggtata tgaagaggaa ttcactcagg tgcgctcatt
     5041 tgcatggagc cctgatggca ataaaatagc ttattacaaa ttcgatgaaa gcgaagtgcc
     5101 ggaatttgat atgatgacgt ataaggggca attgtatccc aaaaattatg aatataaata
     5161 tccgaaagta ggcgagaaga acagcaaggt ggtgatttat atttatgata tcgctacaca
     5221 gaaatctact gtcgtaaata ttgcaaaacc ttatgaatac ataccgcgca tcaaatggac
     5281 aaacgatggc tctgctttgt gtgtatttac gatgaacagg ttgcaaaatg accttgaatt
     5341 atttcttgct gatccggcta caggtaacac cagaagcatg tttcatgaaa cagcaaaata
     5401 ttatttagag ataaatgacg accttacctt cttgaaagac aataaatctt tcatctggcg
     5461 cagtgaacag gatggttgga atcatttata tcgctatgat atgaatggta agctgattaa
     5521 tcagatcact tcaggaaaat ttgaagtaac gcaagtgctg ggtataaatg aagaaaaagg
     5581 tgtggtatac tatatgagta cagaagaatc accttatgaa cgtcatttgt atgcaacgca
     5641 gttagatggt aaaaagacga aaaaaatcac ctctcgcaaa ggcaacaaca gcgtgaattt
     5701 cagtacaggg tttaaatact ttatcaatac caattcaacc tttatgcagc ctgattatat
     5761 cacgttgtgt gatggatctg gaaaggaaat ccgcgtgatg gaagacaatg cagcattaca
     5821 aaaagatgtt gatcaacttg atatagcggc accgcaattc atgcaaattc cgggcgctga
     5881 tggcaacgga ttgaatgcct atatgattaa acctaaaaat ttcgacgcgt ctaaaaaata
     5941 tccggtactg atgacagtgt atggcggtcc cggttcgcag gaagtgattg atggttttga
     6001 tggatttaat atgatgtggc atcaaatgct tgcacaaaat ggatatatta ttgtgtgtgt
     6061 agacaacaga ggaacaggtg caagaggtcg cgatttcagg caagttactt acggtcagtt
     6121 aggaaaattc gaaaccgacg atcagatagc cgcagcgcaa tggttaggta agcaaacata
     6181 cattgatgct tcacgcattg gtatttgggg atggagctat ggcggttata tggcaggatt
     6241 atgtattaca agaggtgctg atgtgtttaa aacggcaatc tcagttgcac cggttacgaa
     6301 ctggaaatac tatgacaata tctacaccga acgttatatg ggaaccttgg aaacaaatcc
     6361 gaccggattc gatgcaaacg cgccaatggc ttatgcagat atgttgaaag gaaattattt
     6421 attggtgcac ggaaccggtg atgacaacgt gcatttccaa aatagtgtgg agtggattaa
     6481 tgcactcgtg cgcaacaata agcaattcga cctgatggta tatcctgata gaaatcatgg
     6541 tatatacggc ggcaaaacaa ggttgcactt gtataccttg atgacagact atatattgga
     6601 aaacttgtaa ggaattacaa ccccaacggg ttttgcatgg ccatgaaaat ggctcttgtt
     6661 tgagcagctt cttttacgtc gtgtacccga agaatatgtg ctcctttttg caataataaa
     6721 gcatgcaatg ccgtgctgcc atttaatgcg tgatcaggag aaatgtgtaa taatttacag
     6781 atcatacttt ttcgggaaac gcctgcaaga acagggcaac caagtgctag gaatttttca
     6841 aaagatgcca gcaattggta attatgggtc agcgttttcc caaatccaaa tccggggtcc
     6901 aatatcacct gttgcaatcc gtgatgtttt aatgaggtgg ttttttctga taaaaaagtc
     6961 aaaacttctt ccacaacatc ctcataatat ggttctaatt gcatggtttg tggatttccc
     7021 tgcatgtgca tgagaatata cggcgcctga tgatcaatcg cggcctggaa aatatcactg
     7081 tccatggtac ctgccgagac atcattgata atatcggcac cggcttttag tgcttctcgg
     7141 gctgttctgc cgtaaacagt atcgatggat atgagcattt caggaaattc tacccttatt
     7201 tcggcaataa ccgggagtac tctgtccatt tcctccctgg gagaaatgat atcagccccc
     7261 ggccttgtgc tcataccgcc aatatcaatg atatcaacac cggcctttac catacgctca
     7321 gcctgctcta cggcacttaa accttccaca aacctgccgc cgtcataaaa ggagtcgggc
     7381 gtgacattca gaatacccat gattgcgggt ttcgagaagt caagcgtgtg cctcgaggtg
     7441 tgtaacgcta ggttagggta gaaatcggta tttttgtcca ttggtaatga gtacgaaatt
     7501 aatgcaatct gacgtgtcta aaacgaattt gcaatatgat gacgttgttg agggctgccg
     7561 caacatctat ctgaagaaga tcaaggatta tggaacctca tggcgtatcc tgcgtccgac
     7621 ctcgctgaca gatcagattt tcatcaaagc acagcgcatc cgaacattgg aacaaacggg
     7681 tgtgagcaga gtgggagagg gaatccacga tgagttcaga ggtattatca attatgccat
     7741 cattgcgctt attcaacttg aaatggggga gagcggccct gtgaacctta gcgaagaaaa
     7801 agcgttggaa ctatacaatg aaaaaatcgg taaagtaaaa aaactgatgc tcgataaaaa
     7861 tcacgattat ggtgaagcct ggcgtgatat gcgcatcagc tcatttaccg acctgatctt
     7921 gatgaaatta ttaagggtaa aacaaattga agaaaacgac ggcaaaacac tgatatccga
     7981 aggtgttgat tcgcactata gcgatatgat caattattcg gtgtttgcct tgatcaaatt
     8041 gagtgaagat gaaaaataaa ctgttgacca tctgccgcat tttaaccggc ggactattta
     8101 ttttttcagg gttagtaaag gccaacgacc ccgtaggatt cggcatcaag ctggaagaat
     8161 attacgatgt atttgcagga cgctggtcgt ggacagcatg gctgtttcat agcgacctga
     8221 tgttaaatac cgtaaacctt caggctgcat ttcttacaac attagaggtg gcgcttggaa
     8281 ttgccttact catgggattg tggaaaaatc tggtgtcatg gttattatta ctgctgattt
     8341 tatttttcac atggctcaca ggttacagtg ccataacagg cagtgtgacc gattgtggtt
     8401 gctttggcga ttttataaaa ctcacgccat tgcaatcatt ctataaagac cttgttttgt
     8461 tggcgttaat attggtgatt ttcttcttgc gcaaaggcct gaaaccaata aagccacttg
     8521 gaattagcat aggtttgttt ttaacggtga ccgtatttgc catatgggtg aatgtgcacg
     8581 tattgcaaca cgatgtattc ctcgactggc gtccatatgc cgttggcgat aatattaccg
     8641 agaatatgaa gttgccggaa aatgcacaac cggatattac ggaaatgata tatgcataca
     8701 ccaataaaaa atccggtgaa caggtgacat tgaaatattt aaataccgag ttgaaggaaa
     8761 agaaaaatct ggatgaattg ctttcttaca gtaataataa agatacctgg aagctcgata
     8821 catcatacac caagataaaa gtaaaaggtg atagggctaa gattatcgat ttcgccatca
     8881 gcgacgaaca tgatgaattt attaccgacc aaattttagg taatccggat tatgcactga
     8941 tggttgtttc tgcaggattt gaacatacga ataaagaggg atgggaccgc atcagcgctg
     9001 ttcaaaagga tgctgagaaa gaaggactat tcacctttgc acttgttggc gaaggaagaa
     9061 cagagattga gaaattccga cacaatcagc aaatggcatt tccgttttat actgccgatt
     9121 ataaggtatg tctcaccatc gctcgcgtaa atccgaatgt agtactgatg aaagaaggaa
     9181 ccatcattgc aaaatgggca tggcgcgatc ttccttcatt cgaggaaatt aaaaaaacgt
     9241 atttccccga aagaaagcca accgaattaa tggtgatttc aaatgagatg tttgctcccg
     9301 gtattcaggt gtcagcaaaa ttgcgggatg cagaagaacc ctacaatgaa ttcttcctgc
     9361 aagatacggt tggcaatgat ctgactactg cactgattaa cgatactaca aatgctgtca
     9421 tgtttatcat caacgaactt agttccgata aacttaccat ggaaaattgg aaatcaattt
     9481 tggctgttat gcaaatgtca aacacagcgc aaaaaccatt tttcgttgcc agttcaggat
     9541 ctcctgaggt attggagatg atgaaaaaag cctctggatt ggaatttggt tattatgttt
     9601 ccgataaaga tgtattggca aaaatgatgg agacgaatac cggattaatc gtaatcaaaa
     9661 atggtaaagt aacagccaag tatgctgatg gtaaactacc ttccgacagc aattttctga
     9721 cccaataaga agtaatcttg attagctata tcatcaaacg acttggttac ggcgtgctgg
     9781 tgctgatcgg tgtggtgata gtggttttct ttctttttca gctgacacgc attcacccgg
     9841 agcgacaaat tgcaggtgaa aaagcagata aagaaacaat agaaaatatc aaaaaggaac
     9901 taggacttga tctgccggta tcacaacaat tattgttgta tttgaatgat atttcgcctt
     9961 tgagttttca tcaggttgca gatagtagtt cccgatattt tttggatggc ggtaagtatg
    10021 attatttcag attgtttact atcaatcata cagctacggt tcttaagaaa ccttatttga
    10081 gcaggtctta cgtgagtaag cgaagtgtga atgaaatttt atcgcagcgc attccgaata
    10141 cattggttct ggcacttgca gccatgttgc tggctaccgt agtaggtatt gcactcggtg
    10201 tgattgctgc cgtaaaacaa ggaaccgcga ttgaccggtt atttactgcc ggaagtgtgt
    10261 tgggtatttc ttttccctcg tttttcgcag gcattatctt gcaattgata tttgcttatt
    10321 tgttggcttc gattacggga tttaatatga ccggcaatct gtacgaaaca gatccttata
    10381 ccggtgaacg ttatctggcc ttgcaaaatc ttattctccc tgccattgcg ctcggtattc
    10441 gtcctattgc ggtaatcacc cagttaacac gaagtgccat gatagatgtg ttgagtaatg
    10501 attatatccg caccgcaaga gcaaaaggcc tttcagaaag aaaaattgta tttcgccacg
    10561 cgctgaaaaa tgccttaaat cctgtagtta ccagtatttc aggttggctg gcaggattac
    10621 ttaccggcgc atttttcatc gaggcgattt ttggatacaa tggcatcggt tatgaaacag
    10681 tgaatgcgct gaaaactttt gattaccctg ttgccatggg tgcaatttta tatactgccg
    10741 tgatatttgt tgtcatcaat attatcgttg atattttata ttctgtaatt gaccctagaa
    10801 ttcgcatcgc atgagagtgt atctgattgg atttatgggt tgcggaaaaa caactttcgg
    10861 caagaaattg gctcggaaaa tgaatgttga ttttatagat ctggataagc aaattgaagc
    10921 gcatgaaggt aaatctgtaa gtgaagtgtt tgccgaaaaa ggggaggcct atttccgcga
    10981 attggaatca tcattattac agcaaactat atctcttaat catgccgtta tctcttgtgg
    11041 cggagggaca ccatgcttcc atgataatat gcattggatg cgtgaacatg gtattaccgt
    11101 ttatttgaaa gttaccccgg aattcctttt tagcagattg catacacgaa gagaaaagcg
    11161 cccgcttatt gcaaaattga atgatgagga attacaagca tacatcacca caaaacttga
    11221 agagcgcgaa gtgcattatc aacaagccca acaagtcatt gatcctgttc atgcaaaacc
    11281 cggctttgtt gccgacctgt tgaatgccag aaagggtaat tgaaaatcct tacattaaat
    11341 atacacctgc atgctgctta tcgctcagca caactttgat gtgctcgtga atggtggtgc
    11401 tcaatgatag tccgacataa tcaggcgtaa caggaaataa tttgtgcttg cgctctacca
    11461 atactacgct gcggattttt ttcggtgaat aagataacaa tggttttatg gcgtacaaca
    11521 atgtgcgacc ggtattggat acatcatcgg caaggatgat aaccttattg tctatctgct
    11581 cttcagtaag atttaattca acaacagaat cacctggatt gtgtttatcg agttcaacat
    11641 gcgttaatat gatatgtgca gatgatattt tctttaattc ctgataaatc aattctgcaa
    11701 aagcataacc ttcggtctct ttgcaaatgc ctgccaatac tatttccggt tcatcgtaat
    11761 tatcttcagc aatttcaaag gcaatacgct ttatcttttg caataccttt tccggaga
//
LOCUS       DAOQOU010000098        11792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2797_length_11792_cov_5.246145, whole genome shotgun sequence.
ACCESSION   DAOQOU010000098 DAOQOU010000000
VERSION     DAOQOU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2797_length_11792_cov_5.246145"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..928
                     /locus_tag="PLM90_09265"
     CDS             320..928
                     /locus_tag="PLM90_09265"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HMZ89603.1"
                     /translation="MFKQFNKESEKLRFETFVDAILAIIMTILVLEFKVPEEPFDSDS
                     SLLDHLSHLIPSVLSYVITFSTIVILWLDHHNLFRMLKKADIPLVMLNFLFILFLSAT
                     PFTTSLSGRNFHNPYAIAIMAANYLLMNISFSAIWVYVQQKKLMDENISAQFNNKFEN
                     IIILIGILLQIACIPLAFVNTAIPFMLFVVILILHLIRLLRH"
     gene            complement(1287..2690)
                     /locus_tag="PLM90_09270"
     CDS             complement(1287..2690)
                     /locus_tag="PLM90_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89604.1"
                     /translation="MKKLFFLLAMSTQVLFAQNIGFNGAGLFENYDRDVEQYLADLNQ
                     PFTIRFPGGSISKFHDPYNVRRGWGMSAENIKDWFQRTGFDEDGNGMEKWVRKAEEQP
                     DHSYMDDLIAMQKRFPQMTVLWVLNVLNSTPEANMQAIRYLMNGGVHIVGVEAGNEIY
                     GKYATFDEYIHDFEPIFKMMRAEYPDVKLALIAGANLNRKELVRWNDAMAQYKGDYDA
                     VVLHYYYTSRELGEAYDMIPLRGKYDPEKPNKDLDKAYAKAADLLAKNDLLGNGIKYA
                     KQQFKGKEIWITEWNTKPSDMLNNTILNGAWQFEQLITMRQQAEYLIIHNGVSPDKYG
                     MISKTNKYDSEKGEMLKRIGYFAYMLAGEAGDAQKISPTEKNVISAPVNGVVRMYFTN
                     TECNPFTPNIDLGKYKGADITINYVAGKHLYSGSGTTGYMGKGSVPSYEVKGITREKF
                     SGSIPAYSFGYLEIRVK"
     gene            2822..4456
                     /locus_tag="PLM90_09275"
     CDS             2822..4456
                     /locus_tag="PLM90_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89605.1"
                     /translation="MQAQQPSGNSYVPLGGDTYHLIDRMDIQYPLSYQYLNTSIKPFL
                     RSDVASLTQTWEHSDTLSKRERFQYDYLGNDNREFMNPHSNDYFEGDLFHFFYPEPAT
                     FYQFFSVRINPVLHNEFGVSSDSSNLRFINTRGLELRGSVDDKVGFYLYATDNQAVLP
                     NYIQDRVDAAPQVMPGDGVTKIFKDNGVDYFTTHGYISFNPTKHIEMQFGQDKMFIGD
                     GIRSMIWSDNAKDQLMLRINTHLWRLHYLNVFTELANYDGSNIYNSLIHKKYAAFHYL
                     TVPIIPQIEVGVFESIIFDRYNANGYETGFELNYLNPVIFYRAVESGLGSADNALVGA
                     TLKTKLWNTASLYGQLVLDEFVFGELFGGNKWWGNKYALQAGAKYINAFNIEQLDLQY
                     EFNMARPYTYSYEDDNGSSYTHYAQAIAHPLGANFKEQLFRVWYQPARKLTIRNTTMI
                     AQQGIDSAGINYGSNIFLDYNTYASAYNNITGQGISQTTLLNDLLVSYAFWHNTFLDL
                     HITYRDVNSELVENDNNELYFSIGVRMNEGVRGFMW"
     gene            complement(4879..5157)
                     /locus_tag="PLM90_09280"
     CDS             complement(4879..5157)
                     /locus_tag="PLM90_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89606.1"
                     /translation="MRQGKAMSIYFNPLFWIPIIFSIGLIVFGVVLITDNPDNTANLG
                     EVGGNSEAASTVAQSLMQTLQPAFTLFGILIIILGVCIGGRFLYKIGR"
     gene            complement(5264..5917)
                     /locus_tag="PLM90_09285"
     CDS             complement(5264..5917)
                     /locus_tag="PLM90_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89607.1"
                     /translation="MKKHLIFFCCLTLYFQQLSAQTPFETTIPDTAEIKKWIHIKGHI
                     VDELLWTDSLGENLLITTETGDFISPNNPEQVDGEDAELYAYHYLFAIDGAIETWKVY
                     DYIHLCPVDMIAAFVDSTTHITDLDSNGIAEIWLMYRTVCHGDLSPLTMKIIMYESDQ
                     KYALRGDTKIYMGTDDKGVKHYMGGDYKADAAFINGPPVFLQHAISMWEKHLVVYDY"
     gene            complement(6110..7003)
                     /locus_tag="PLM90_09290"
     CDS             complement(6110..7003)
                     /locus_tag="PLM90_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HMZ89608.1"
                     /translation="MMLQERSIGIKIAEARKKQNLSQAELGRLISISPQAVGKWERGE
                     SMPDITTLNRLAEILGVDLNYFSENFPSIIDQHPTEQLAPLTDSPLIRIKKKLNRNMS
                     AGNWVDADFSGIRDLQEKFSGSNMKNCLFRNADLSGLTLASNNIDKCDFTGTDLRDSS
                     IRSSNLMLNNFSSCSLIDTQFVRNNIGSCNFTETNFSGAEFTSCNFEKNDLSGAVWKF
                     TTFKNCAFSEIIFEGPMADCHFENCSFYHVTFQTALLTNTFFKYNRRFKKVKFIDCKV
                     DSLTYAFLKNNQADLSGVVVV"
     assembly_gap    7398..7497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7708..8043)
                     /locus_tag="PLM90_09295"
     CDS             complement(7708..8043)
                     /locus_tag="PLM90_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMZ89609.1"
                     /translation="MPYKEKEIEKVYWSIGEVAEMLDLSFAQIRLWEKEFDVFQLKKN
                     KKGDRYFTKKDIEQLKLIKYLLKEKKYTIKGAREQLKSKKPQTDSRFQTIEALKKIRQ
                     FLVELKEQI"
     gene            complement(8183..9157)
                     /locus_tag="PLM90_09300"
     CDS             complement(8183..9157)
                     /locus_tag="PLM90_09300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ89610.1"
                     /translation="MAKKVKYFYNPANLRFEKHVTPRWVWTLRIIGWISTAAVFAFII
                     VWIAYTYFDSPKEQYLKNQISEMELELDFMNSKLDTMAVVLGEMTERDDNIYRVIFEA
                     EPVSRSERMAGIGGSERFQRMNKLDNAELLINTATKLERIRRQMVIQSKSFDEISELV
                     SNKEQLLSHTPSIQPVSNKDLERIASGFGWRIHPIYKFAKFHEGLDFTAPKGSDVFAT
                     ADGTVVLADNSQSGFGNCIVIDHGYGYKTRYAHLSAFKVRAGQKIKRGELIGLVGNTG
                     LSTAPHLHYEVEKNGTKIDPINFFYNDLSAEEYARMIEIANRSNQSYD"
     gene            9272..>11792
                     /gene="alaS"
                     /locus_tag="PLM90_09305"
     CDS             9272..>11792
                     /gene="alaS"
                     /locus_tag="PLM90_09305"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMZ89611.1"
                     /translation="MMTSREIRQQFLQFFERQQHKIVASAPIVVKNDPTLMFTNAGMN
                     QFKDLFLGNKPVQYPRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYF
                     KKESITWGWQLLTDIYKLDPSRLYVTVFEGDPSENLAFDTDAYDVWKSMISEAHILRG
                     NKKDNFWEMGDTGPCGPCSEIHYDMRGDAERSAIDGAALVNTGDPQVIEIWNHVFIQF
                     NRKADGSLEELPNKHVDTGMGFERLVRALQGKSSNYDTDVFTPLIRKVETLSGFQYGQ
                     DEKTDIAMRVISDHIRAVSFCIADGQLPSNNGAGYVVRRILRRAIRYGYSFLHLKKPF
                     FNELVAVLAEQFRDVFPELEAQRDLVSRVIMEEERAFLRTLENGIKRLDDVTRSEQVK
                     SAKIIPGAVAFELFDTYGFPLDLTMLIVREHGLDVDSAGFEAEMQKQKERSRQATGME
                     TGDWMVLRDDKSVDFIGYDHLQSESRVVKMRSVKMKNEMQYQVVLNKTPFYAESGGQT
                     GDTGTLSINANIIRVLDTKKENDLIIHFTDQLPENTDAVVYAEVDARRRTDITYNHTA
                     THLIHAALRQVLGSHVQQKGSYVGPDRLRFDFSHFSKVSDEELRRIEQIVNAKIRENI
                     PVVTKVMPIAEAQQLGAMMLFGEKYGETVRVVIADENYSKEFCGGTHVGATGEIGYCR
                     LVNESAVAAGIRRIEAITGSAIEKYMDEQHELLKKIQDITKVSDVVKSVQSLADENAA
                     LKKEVEQFQLLQVQQVKASLLNAIETTGGMNIIRAQVDLGNADLIKKLAYELRNEVSD
                     LVCLLAADIQGKASLTLMISDNLVESKGYDAAKLIRELGKQI"
BASE COUNT         3336 a         2489 c         2424 g         3443 t
ORIGIN      
        1 tacctgatac ttgatacctg atacttgata cctgatacct gatacctgat acctgatact
       61 tgatacctga tacctgatac ctattacttg atacctgata cttgatacct gatacttgat
      121 acctgataca gactattaat ttcatcgcca caatgaatca accgcgcccc tccgcttccg
      181 cagcgtgaaa aaaacgaacc ataaacaaca caagctcccg tttcaaatgc aatagctgct
      241 catttcaata tgcattagaa tccttaattt tacggtacac attaaaccca ttcttcggtc
      301 aaccccaaca cacctaacta tgtttaaaca gtttaataag gaatcagaaa aactgcgctt
      361 cgaaacattt gttgatgcca ttcttgccat tatcatgacc atactggtcc tggaatttaa
      421 ggtacctgaa gaaccatttg actctgacag cagcctcctt gatcatctat cacatttaat
      481 cccttccgtg ctaagctatg ttatcacctt ttcaactatc gtgattttat ggctcgacca
      541 tcacaattta tttcgtatgc ttaaaaaagc cgacattccg ctggtgatgt tgaatttctt
      601 attcatattg ttcctttccg caactccctt tactacatca cttagcggtc gtaattttca
      661 taatccctat gcaattgcca tcatggctgc aaactatctg ctcatgaata tttcgttctc
      721 agccatatgg gtttatgtgc agcaaaagaa actaatggat gaaaacattt ccgcacagtt
      781 caataacaaa tttgaaaata tcattatcct aatcggcatt ctgcttcaaa tagcatgcat
      841 tccactcgca tttgtgaata ctgcaattcc attcatgtta tttgtagtaa tacttatttt
      901 acatttgatc aggctattga ggcattgaag gagagaggag agaggagaga ggagaaagga
      961 gagaggagag aggagattgg gtggagatgt tatggattgt gtttttttgt ggatttgcgc
     1021 agagaaaatt tatttggctg cgcacaaggt cactgcagta aaccaaccgc gttcctccgc
     1081 ctcgccttgc cctctgcgtg aaataaaaca cataccacaa gcgcgccacc ctacctcatt
     1141 ttatttagaa ctttgagcct ctcggcattg aagaaaattt cattcgatgg atatatttcc
     1201 ttgtaacagc acaaagtcta attacccaac aactacttct tgcagccgag taattagact
     1261 tgaaataatt tatttgaaaa acgtaattac ttcaccctaa tctctaaata tccaaagcta
     1321 taagcaggaa tcgagccgga gaatttttct cgtgtaatgc cttttacttc gtaactgggc
     1381 acactgcctt ttcccatata gcctgtagtt cccgagccgc tatataggtg ctttccggca
     1441 acatagttaa ttgtaatatc agccccttta tattttccga gatcaatatt tggcgtaaac
     1501 ggattacatt ccgtatttgt aaaatacatg cgcacgacac cattaaccgg agcactaatc
     1561 acattctttt ctgttggtga aattttttgc gcatcaccgg cttctcctgc aagcatataa
     1621 gcaaaatagc cgatgcgttt caacatttca cctttctcgg aatcatactt attcgtctta
     1681 ctgatcatgc catatttatc gggactcacc ccattatgaa tgatcagata ttcggcttgc
     1741 tgacgcatgg tgatcaattg ctcgaactgc catgccccgt tcagtatcgt attgttgagc
     1801 atatcggaag gcttggtatt ccattcggta atccatatct ccttaccttt gaattgctgc
     1861 ttcgcatatt taattccatt tcccagtaaa tcatttttcg ccaataagtc tgcagccttt
     1921 gcgtacgcct tatcgaggtc tttattgggt ttctccggat catactttcc acgcaaagga
     1981 atcatatcat aagcttcacc caattcgcga ctggtataat aataatgcaa taccaccgcg
     2041 tcatagtcgc ccttatattg cgccatggca tcgttccagc gcacaagttc ttttcgattg
     2101 agattggcac ctgcaatgag tgctaatttc acatcaggat attcggcacg catcattttg
     2161 aaaataggct caaaatcatg gatatactca tcgaaggtgg catatttacc atagatttca
     2221 ttccctgcct cgacaccaac aatatgcact ccaccattca tcaaatatcg tattgcctgc
     2281 atattcgctt ccggtgtact attaagcacg tttaacaccc atagcactgt catctgcgga
     2341 aatcgcttct gcatggctat cagatcatcc atataactgt gatcgggctg ctcttctgct
     2401 ttgcgcaccc atttttccat gccattgcca tcttcgtcaa aaccggtacg ttgaaaccaa
     2461 tctttgatat tttctgcact catcccccac cctctgcgga cattgtatgg gtcatggaat
     2521 ttgctgatgg aaccgccggg aaagcgaatg gtgaatggtt gattcaggtc ggcaaggtat
     2581 tgctcaacat cccggtcata gttttcgaaa aggccggcac cgttgaatcc aatgttctgt
     2641 gcgaacagga cctgtgtgct catggcaagt agaaagaata gttttttcat cttggggttt
     2701 tttagtaact ctgcaaaaat taacaatatc cgtgcaaatg tcgtgcctca ggtggcattc
     2761 cgcctgcaaa tgtagaattt tccttttggt aagcctgctg atatgcagtg gaaacaaggt
     2821 aatgcaggcc caacagcctt ccggtaacag ctatgtaccg cttggcggcg atacatatca
     2881 cctgatcgat aggatggata tccaataccc cctctcctac caatatctga acacctcgat
     2941 taaaccattt ttacgcagtg atgtggcatc gctgacccaa acatgggaac acagtgatac
     3001 actttcaaag cgcgagcgat ttcaatacga ctatctcggc aacgataatc gcgagttcat
     3061 gaatccgcat tccaacgatt attttgaagg cgatctgttc catttctttt atcccgaacc
     3121 tgctactttt tatcaatttt tttctgtgcg tataaatccg gtattgcaca atgaattcgg
     3181 tgtttccagc gatagttcaa atctcagatt tatcaatacc cgtggattgg aattgcgtgg
     3241 cagtgtagac gataaggttg gattttattt gtatgccacc gataatcagg cagtgcttcc
     3301 caattatatt caagatcgcg ttgatgccgc accacaagta atgccgggag acggtgtgac
     3361 caaaatattt aaggataatg gggttgatta ttttaccaca cacggctata tcagcttcaa
     3421 ccctactaag catatcgaaa tgcagtttgg acaggataag atgtttattg gcgatggcat
     3481 tcgcagtatg atatggagcg ataatgctaa agaccagctc atgttacgca tcaatacgca
     3541 tctgtggcga ttgcattacc tcaatgtatt taccgaactc gctaattacg atgggtcgaa
     3601 tatctataat agtctcatcc acaaaaaata tgcagctttt cattatctca cagtacctat
     3661 catcccgcaa atagaagttg gtgtatttga gtctatcatt tttgatcgat ataatgccaa
     3721 tggttatgaa accggattcg aattaaatta tcttaacccg gttatcttct atcgcgcagt
     3781 tgaaagcgga ttgggcagtg ccgacaatgc cctggtaggc gcaacgctca aaacgaaact
     3841 gtggaacacc gcctcactat atggacaact tgttctcgac gaattcgttt tcggcgaatt
     3901 atttggcggc aataaatggt ggggaaataa atacgcgtta caagcaggtg caaaatatat
     3961 caacgcattt aatatcgaac aactcgatct gcagtatgaa ttcaatatgg cgcgaccata
     4021 tacctacagc tacgaagacg acaatggctc cagctataca cactatgcgc aggcaatagc
     4081 gcatccgctt ggagcgaatt tcaaggaaca actatttcgc gtctggtatc aacctgcccg
     4141 caaactcacc atacgcaata ccaccatgat tgcgcaacaa ggcattgaca gcgccggcat
     4201 caactacggc agcaatatct tcctcgacta caatacttac gcttctgctt acaacaacat
     4261 caccggacaa ggtatatcac aaaccaccct tctcaatgat cttctggtat catacgcttt
     4321 ctggcataac acctttctcg acctacatat cacctaccgc gacgtaaaca gtgagcttgt
     4381 cgaaaatgac aataatgagc tttactttag tattggggta aggatgaatg agggggtgag
     4441 ggggtttatg tggtaggaga ttaattagct aatttgagaa ttagctgatt agcaaattta
     4501 ggtaattatt tgcatggaga caacagcttt agtttgcaag tttgaaagtt ggcaagttag
     4561 caaattggga attagaaaat ttgagaatta gcaagtggtg gtgcttttaa cgctggcgcg
     4621 ttaatagagt atgtaattat tttttttgca aaattttatt accgaagagt aattagctcc
     4681 ctctctatcg acggtaggag atggagaggg cggggggtga ggtgagagtt ggcaagttag
     4741 caaagtggga attagctaat atgagaacta gctgattagc aaattgaggt aattatttgc
     4801 atggagacaa cagctttagt ttgcaagttt gaaagttggc aagttagcaa attggggtga
     4861 ttgcttgcat gggttacatt atcttccaat tttgtaaaga aatcttcccc ctatacatac
     4921 acccaaaata ataatcaata ttccgaataa ggtaaatgca ggctggagag tctgcatcaa
     4981 tgattgcgct accgtcgatg ccgcttcgct gttgccgcca acctcaccga gattggctgt
     5041 attatccgga ttatccgtaa ttaatactac accgaaaaca ataagtccta tcgagaaaat
     5101 gatagggatc cagaataatg gattaaaata aatgctcatc gccttccctt gtctcataaa
     5161 taattacctt aaaactacta tagaactaaa ttaaaacatc aacgacacat tggcacagga
     5221 aactttgtat caatttataa aacatttcaa gaatttggcc gggctaataa tcataaacaa
     5281 ccagatgctt ctcccacatg gagatggcat gttgcagaaa tacaggtggg ccgttgatga
     5341 atgcagcatc agctttgtag tcaccaccca tatagtgttt tacgcccttg tcatctgtgc
     5401 ccatataaat ttttgtatcg ccgcgcaggg catacttttg atctgattca tacatgatga
     5461 ttttcatagt taacggactc aaatcgccgt gacaaacggt tcgatacatc aaccatattt
     5521 cagctatgcc gttgctatcg aggtcggtga tatgtgtggt gctgtcgaca aacgctgcaa
     5581 tcatatctac ggggcataga tggatataat catatacttt ccaggtttca atcgctccat
     5641 caattgcgaa taaataatga taggcatata attctgcatc ttcgccatca acttgctccg
     5701 gattatttgg gctgataaaa tccccggttt cagttgtaat taatagattc tcgcctaaac
     5761 tgtcagtcca tagcagctca tccacaatgt gaccttttat atgaatccat tttttaattt
     5821 cggcagtatc gggaattgtt gtttcgaaag gtgtttgggc ggataattgt tgaaaataaa
     5881 gagtgaggca acagaaaaat atcaagtgct ttttcatgct attaaaataa aaagcatcag
     5941 atgatccgga atgcattttg cagggaattc tattcatgat ttgggaacct ggttactaag
     6001 ttaattgatt ggcatgaaat aagggcactg caagctgaat tataagctat taccaggccg
     6061 cagtcaggaa ataaaacgac aattcaacca aactattatg caaaatcatt cataccacca
     6121 ccaccccact tagatcggcc tggttgtttt tgagaaaggc gtaggtgagt gagtcaactt
     6181 tgcaatctat gaattttacc ttcttgaagc ggcgattata cttgaaaaac gtatttgtaa
     6241 gcagcgcggt ctggaaagtt acgtgataaa acgaacaatt ctcaaagtga caatcagcca
     6301 taggcccttc gaaaataatt tcgctgaagg cacagttttt aaaggtggta aatttccaaa
     6361 cggctcctga taaatcattc ttctcgaaat tacaggaggt aaattccgct ccggagaaat
     6421 ttgtttctgt aaaattgcaa cttccgatat tatttctcac aaattgtgta tctatcaaag
     6481 agcaactcga aaaattattc agcatcaaat tcgacgagcg aatgctactg tcgcgaagat
     6541 cagtgcctgt gaaatcgcat ttgtcgatgt tattactggc gagtgttaac ccggataggt
     6601 cggcatttct gaacaggcag tttttcatat tagacccgct gaatttctct tgcaggtctc
     6661 tgatacctga gaaatcggca tcaacccaat ttccagccga catattccta ttcaatttct
     6721 tcttgatcct tatcaagggc gaatccgtta aaggcgcgag ctgttctgta ggatgttgat
     6781 caataataga agggaaattt tcagagaaat aattcagatc aacgccaagg atttcggcta
     6841 agcgatttag tgtggtgatg tcgggcattg actctcccct ctcccatttc ccaacggctt
     6901 gtgggctgat agatatcagt cgacccaatt cagcttgcga taaattctgt ttctttctgg
     6961 cttctgcgat cttaattccg atcgatcttt cttgtagcat catatgtttt gttttgaagt
     7021 cgcaaagcta ttttgaccac aggttgcatt acaaatatac atcgctactg ttagtggtaa
     7081 agtagctacc gttggttgtt tttcacgcag agaccgctaa gacaccaatt gtgttcatgt
     7141 gtttgtgtgt tcaggttcgg catttacatt cagcagcaca cctgctaact tgctaaccct
     7201 caaacttgct cactaaagcg aaaaactcca tatgcttaca agcctatttg ctaatcagct
     7261 aaatacttct gtatggagcg caccgacctc ccgacgggac ttacttactt gctcacatgc
     7321 acacatgtac acttgagcgc cttcggagcc cataccccat gtaactattc accaaatcag
     7381 ctaatcggct aatcagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg
     7501 gagcccatac cccatgtaac tattcaccaa atcagctaat cggctaatca gcaaatcggc
     7561 taattagcta atcggctaat cagctaatcg gctaatcagc taatcagcta atcggctaat
     7621 cagctaatcg gctaatcagc taatcggcta atcagcaaat cggctaatca gctaatcggc
     7681 taattggcta attagctaat tagctaatca aatctgctct ttcagttcaa ccaaaaactg
     7741 tctgattttc ttgagcgctt cgatggtttg gaatcgcgaa tctgtttgag gttttttgga
     7801 tttcagttgc tcacgtgcac ctttgatggt atactttttt tccttgagca gatatttaat
     7861 tagcttgagt tgctctatat ccttctttgt gaagtaacga tcgccctttt tatttttttt
     7921 gagttggaac acatcaaatt ccttctccca gagcctaatc tgcgcaaaac tgagatcaag
     7981 catctcagca acctcgccga tcgaccagta gactttttct atttcctttt ctttgtaggg
     8041 catgataagg actgccgggt gtgtataagg gtttgaagaa caaatatata gtatttgatt
     8101 ggtaaagtac ttagggaggt agatcgctag tttttcacgc agaggcgcag agccgttgag
     8161 aagtctgata ccggataccc ccttaatcgt aggactgatt actgcggttg gcgatttcga
     8221 tcatgcgggc gtattcttcg gcgctgaggt cgttatagaa gaagttgatg ggatcgattt
     8281 tagtgccatt cttttctact tcgtagtgga ggtgcggcgc agttgaaagg cctgtatttc
     8341 caactagtcc tatgagttca ccgcgcttga ttttttgtcc tgcacgcact ttaaatgcac
     8401 tgaggtgagc atatcgcgtt ttatatccat aaccgtgatc gattacgata cagtttccaa
     8461 agccactttg agaattatca gctaatacca cagtcccatc agccgttgca aacacatctg
     8521 aacctttagg agcggtaaag tcgagccctt catgaaattt tgcgaattta tagatagggt
     8581 gaatgcgcca tccaaagccc gaagcaatgc gttcaagatc tttattgctc accggctgga
     8641 ttgaaggcgt atgactgaga agttgctctt tattgctcac caattctgat atctcatcaa
     8701 acgatttcga ttgtatcacc atttgtcggc gtattctttc gagtttcgta gcggtattta
     8761 ttaacagttc ggcattgtca agtttattca ttcgctgaaa gcgttcactt ccgccgatac
     8821 ctgccatacg ctcgcttctg gaaacgggtt ccgcctcgaa tatgacgcga tagatgttat
     8881 cgtctcgttc agtcatttca cccaatacca ccgccatggt atccagctta ctgttcatga
     8941 aatcgagctc cagctccatt tcggagattt ggttcttgag gtattgttct ttaggagaat
     9001 cgaaataggt ataggctatc caaacgatga taaaggcaaa aacagcagcg gttgatatcc
     9061 agccaataat gcgcaaggtc cacacccacc gaggtgtaac atgcttctcg aagcgcagat
     9121 ttgccggatt atagaaatat tttacttttt tcgccatgcg ccttacctgt gtaattccgt
     9181 aattttgcgg ctcgccgggg aattgtgctt tagaggggca acgaaaatac agcttcccgg
     9241 ccaaaaaata aaacgaaaac cggatatatc gatgatgacc tcccgagaga taagacagca
     9301 gttcctgcaa ttttttgaac gccagcaaca caagatcgtt gcgtcagcac ctattgtggt
     9361 gaagaacgac cctactttga tgttcactaa tgcgggcatg aatcagttca aagacctatt
     9421 ccttgggaat aaaccggtgc aatatccgcg tattgcagat actcaaaaat gtttgcgcgt
     9481 ttcaggcaag cacaacgacc ttgaggaagt tggtgtagat acctatcacc ataccatgtt
     9541 cgaaatgctc ggaaactgga gttttggcga ttattttaag aaagaaagta ttacctgggg
     9601 atggcaatta ctcaccgata tttataaact cgatccttcc agattatatg tcaccgtttt
     9661 tgaaggtgac ccgtcagaga atcttgcttt tgatacggat gcatatgatg tttggaaaag
     9721 catgatcagt gaagcacata ttttgcgtgg taataaaaaa gataattttt gggaaatggg
     9781 tgacaccggc ccgtgtggac catgcagtga gattcattat gatatgcgtg gtgatgccga
     9841 acgcagtgcc attgatggtg ctgcactcgt aaataccggc gatccgcaag tgatagaaat
     9901 ttggaatcac gtttttattc aattcaaccg caaagctgat ggaagtctgg aagaattgcc
     9961 gaataaacat gtagataccg gaatgggctt cgaacgattg gtgcgcgcat tgcaaggaaa
    10021 atcatcaaat tatgatacgg atgtatttac accgctgatc agaaaagttg aaacacttag
    10081 tggttttcaa tatggtcagg atgagaaaac ggatattgcc atgcgtgtta tcagtgatca
    10141 tattcgtgcc gtaagttttt gtatcgctga tggtcagctg ccttcgaaca atggcgccgg
    10201 ttatgtggtg cgtcgaattt tacgcagggc tattcgttat ggatattcat tccttcattt
    10261 aaaaaaacca ttcttcaatg aattggttgc ggtacttgca gaacaattcc gcgatgtatt
    10321 tccggaattg gaagctcagc gcgatttagt atctcgcgta atcatggaag aagaacgcgc
    10381 atttttacgc acgcttgaaa atggtataaa acgactggat gacgtaactc gctctgaaca
    10441 agttaaatca gctaaaataa tcccgggcgc agttgccttt gaattatttg atacctacgg
    10501 ttttccatta gacctcacca tgctcattgt gcgcgaacat ggactggatg tcgacagtgc
    10561 cggatttgaa gctgaaatgc aaaaacaaaa agaacgcagt cggcaagcta caggcatgga
    10621 aaccggcgat tggatggtgc tgcgcgatga taaatctgtt gattttatcg ggtacgatca
    10681 tctgcaatct gaatcgcgcg tggtgaaaat gcgttcagtg aaaatgaaga atgaaatgca
    10741 atatcaggtg gttctgaata agacaccttt ttatgcagaa agtggagggc aaaccggcga
    10801 tacaggaact ttatctatta atgcgaacat tattcgtgtt ttagatacca agaaagagaa
    10861 tgatctcatt atccatttca ccgaccagtt gcctgaaaat actgatgctg ttgtgtatgc
    10921 tgaagtagat gcgcgtcgcc gaactgatat cacttataat cacaccgcaa cacatttaat
    10981 tcatgctgct ttacgacagg ttttaggttc acatgtacaa caaaaaggtt cctatgtggg
    11041 acctgatcgt ttgcgtttcg acttttcgca tttttctaaa gtgagcgatg aagaattgcg
    11101 tcgcatagag caaattgtaa atgccaagat tcgtgaaaat attcctgttg tcacaaaagt
    11161 gatgcctatt gcagaagcgc aacaattggg tgcgatgatg ttgtttggtg aaaaatatgg
    11221 cgagactgtt cgtgtggtta ttgctgatga aaattatagc aaagaatttt gtggcggcac
    11281 ccatgttggt gcaaccggcg aaatcggata ttgccgtttg gtaaatgaaa gtgctgttgc
    11341 tgccggaata cgacgtattg aagcgataac aggttccgct atcgagaagt acatggatga
    11401 gcagcatgaa ttgctcaaaa aaatacaaga tatcaccaaa gtttccgatg ttgtaaaaag
    11461 tgtgcaatca ctggctgatg aaaatgcagc gctaaaaaaa gaagtagagc agtttcagtt
    11521 attgcaagtt cagcaagtga aagcttcttt attgaatgcg atagaaacta ccggtggcat
    11581 gaatatcatc cgtgctcagg tcgatctcgg taatgcagac cttattaaaa aacttgctta
    11641 cgaactccgc aacgaggttt ccgatcttgt ttgcctgctg gcggccgata ttcagggcaa
    11701 ggctagcctg accttaatga tcagcgataa ccttgtggaa tcaaaggggt acgatgcggc
    11761 aaagctgata cgtgagttgg gtaagcaaat tg
//
LOCUS       DAOQOU010000099        11735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2820_length_11735_cov_6.231764, whole genome shotgun sequence.
ACCESSION   DAOQOU010000099 DAOQOU010000000
VERSION     DAOQOU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2820_length_11735_cov_6.231764"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLM90_09310"
     CDS             complement(<1..336)
                     /locus_tag="PLM90_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89612.1"
                     /translation="MLTFTTVNDPNSASRKQDHIELAFKSQAAAAENDRRFYYEPVMG
                     FHRAEVSLKTSFLGKTLGMPLWISSMTGGTEQAGTINKRLAEACAEFGLGMGLGSTRR
                     LLDDNTWFED"
     gene            complement(481..936)
                     /locus_tag="PLM90_09315"
     CDS             complement(481..936)
                     /locus_tag="PLM90_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89613.1"
                     /translation="MPIAMTLFVLVLLGFIIGSFIDGSITPSIGIFAFMLFFIFLLIW
                     IVFGELRTKVIRVELKDDAVMVSSYLGLGKKRMYNLTQFEGFETAILPSNYDKYEYLY
                     LMTNGKKVVKLSQFYHKNYNELKTVIEGTVTNLGEKSFSMIEEFKEIFQ"
     gene            1310..3007
                     /locus_tag="PLM90_09320"
     CDS             1310..3007
                     /locus_tag="PLM90_09320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HMZ89614.1"
                     /translation="MKFSSFPYHRPDMSALQAQFEVALGKFEHAASAENQLTAMQEIN
                     AVRSAFDTMYQICSIRHSIDTRDTFYTEEQAYFDEHGPEMEALISKYYQVLVKARFRE
                     AIEQKYGKQLFRIAEMQLRAFHPDIIPDLINENKLNTAYQELVASALIPFDGAERTLA
                     EMGPYMKSVDRNVRERAAAARWQFFEDNRAKFDTIFDDLVKTRHRIAKTLGFDNFIAV
                     GYLRMQRSDYNAEMVDVFREQVRKYIVPLATNLRNQQALRLGISTLKYYDEGLQFTSG
                     NATPKGSPEFIIGKGGEMYREMSPETAKFFHEMLDNELMDLVAKKGKAGGGYCTYISG
                     HRAPFIFSNFNGTSADIDVLTHEVGHAFQVFMSRGFDTPEYFWPTSDAAEIHSMSMEY
                     FAYPWMDQFFNGEADKYRYAHLVESLQFLPYGVAVDEFQHRMYAEPELTPLQRKNIWR
                     EIEKKYLPHRNYDGNIFLEEGGFWQTQAHIYQSPFYYIDYTLAQMCAFQFWMMAQEDT
                     TKAFNNYVELCKAGGSKSFLELVAYAKLKSPFDPQAFADTIGHIDNYLKGVDDLQLN"
     gene            complement(3093..4583)
                     /locus_tag="PLM90_09325"
     CDS             complement(3093..4583)
                     /locus_tag="PLM90_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89615.1"
                     /translation="MKKQDTSLFDLIKSMSMAEKRYFKLFATRHFPKDSTYHRLYEAI
                     DAQDKYREQTLTKQFATGKSASYFPVLKKQLYDALLEALHRFEAYDDEVHKLTLGVHY
                     CSLLLNRGLYAACAKQIKKYKAKGYAFEKFEEVIALIAIEKRLAARTQYTDYNHNGLA
                     ALHREELHCLEQIKITSEYWINSAAIYKMHIEKKLGPGRDNHALKSLAASPLFADASS
                     ATTFRARLDRMQVLALYAFAEQQTETAFDYNNAFLQLMDDQPYLRQQYADRYFATLNN
                     YLIDCLILQKHEALIAGIQTLRKLPEEDAFRHMQHLDAQVFRMSYLLELNYYVGLEDF
                     VHALSVATAVLAQITNVVPFIARPNLITLYYLCAYVLFCNKQFDQCLDALQTLMDMKD
                     AETVIDLFTDSRMMQILCHFELGNILLIDSLIVSFNRHLESRKLKASTYATVLRALKQ
                     HMRSPLKPLKQKLHQQLQALSQQPEEKRLYNNFNYFWWLERVSSLQ"
     gene            4730..6844
                     /locus_tag="PLM90_09330"
     CDS             4730..6844
                     /locus_tag="PLM90_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89616.1"
                     /translation="MKKMYTIIAAILLSTAVLPLGAQSWVNYSNLSYVTDAEPSGTDI
                     WITAKGGVQLFNTLTGTSMFFRKGDAGLPSSSVEQVAKSNATGVIWVGTYDAGVVEWD
                     GDSWLTYPFPSAMLLYRMKFDGFGNLWLQTDAGLFHFNTTDHVYTFVNSTGGAGWDFN
                     AWDFDITPDNKVIIFTGTNCQVIDAATLAAIDSFPNSDSPNVLACSPSNVRVYNVDEE
                     TYLINNGFAIEFEFKDNTFTPATDGLPEFAFVQNIIRGTDGALYAYVNNASVYRLDGL
                     TWTYVDNAAVYFPEFLVYTDGVEFYFSSNNYASTPGVIHMSETDYVKISSLEYAFTSN
                     AIKGLTEDADGNIFILSGSGQYQYNELTDDFSLFGNVPSIYGTIYDLRSVNGIFYCVN
                     YGDLLEYNNGTSWVNIPYADGYTSPYIFDYDVTSDGTIYFVNDDGAFKYKDGETTCLF
                     PTPSLYDWGLSVTYDATRNLLWIGRMDGILKYDFVTQELIDAMDVPAMSDGMAIQEIK
                     VAPNGDVWFGANNNKAYMYDGTEWTDYNVGTGMGFVTSFDFEGANIYFGITDGNGGVH
                     VWDTSNDNWSWLSSATDPTLVSDEVSYMTVTTGGDLWIAHVNAGVSRLDGTPSVGIDE
                     VTANNAIIYPNPVAQTLFVSGLEDGNYQYVISDLSGKQLWSSSQATHSINVSQFPSGI
                     YLLQVRAGDKSVFNGTFIVPTK"
     gene            complement(6947..7657)
                     /locus_tag="PLM90_09335"
     CDS             complement(6947..7657)
                     /locus_tag="PLM90_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ89617.1"
                     /translation="MEEIKTKILLVEDDPNFGAVLKDYLTMNGYEVRLATDGEKGEDL
                     FFKEEFNLCVLDVMMPKKDGFSLAAQIKRVNPDQPVIFLTAKTLQDDVLEGFKTGADD
                     YITKPFNSEELLYRIAAVLKRGGKARTNNQQKEFNVGKYHFNYKTRMLKNGDSEAKLS
                     PKEAELLRLFCMKVNDIVYREEALKQIWGRDDYFTARSMDVFVTKLRKYLKDDPTLEI
                     VNIHGNGFRLNAEETANI"
     gene            complement(7676..9250)
                     /locus_tag="PLM90_09340"
     CDS             complement(7676..9250)
                     /locus_tag="PLM90_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ89618.1"
                     /translation="MSTALLGIILVQVYWIQNTIEQKEQIFNYHVNEALNRVADKVET
                     NIAASVLSSQMNLFFSDSTYWMGTGDSLTTYENVLSDSTMAGMLESLRSNQAYYNDPE
                     YTTIEPFADALQGMPGSQKPTMILEPLELIGDNSSPNDALVSQILSDIDRQLQLNSGR
                     IKKAMEQMMIRMVQRGITPEQTIDTNFLKNSLYHELNNRGITTDFNFGVLMHDKFFIS
                     NAEDNFGLEKLAASSHKVSLFPDDIVFNNDVLLVSFPHQKSFILSSIWSLLLGSLLFT
                     TIIVAVFTYTIFILVKQKKMSEIKNDFINNMTHEFKTPLATISLAVDAVNNPMILQDQ
                     QKVKYYTGIIRDENKRMNAQVEKVLQMALLDKHQVSISNDDVDIHEIIYRAVENISLL
                     VEEKGGTVETELLADRFEITGDEVHLSNVISNLLDNANKYTPEHPHIVVRTRSDNKGI
                     YITVEDNGIGMSNDDMSMIFEKFYRVPTGNVHNIKGFGLGLTYVKTVVEAHKGSIEVK
                     SQLKKGSQFRIFLPHQ"
     gene            9453..10910
                     /locus_tag="PLM90_09345"
     CDS             9453..10910
                     /locus_tag="PLM90_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ89619.1"
                     /translation="MQRFLFSITTLIIPAMLFAQQPDNQIHLRIDANVDGQQVKIDTF
                     IESLGDLNLDEFLQGLGLENELNQLNIDINSGFGVMPSFDEEAMQEMLKSLSELEMPE
                     MPELPELPGIDMGEAMSFGYAGENRAFLGVYTNKVTEGAQISDIVEGSAAESAGLMEG
                     DIITHIDARSIESPANLMEVLALYYPGDEVTVTYIRAGKTDKVKLTLQENTNASSTWG
                     SQNYNFNFDFPDSIFMGQGNYLIETPAHGYLGVYLEDADGKVLVTGIEGGSAAEKAGL
                     KADDILLEIKGNKVQSYDEVLEIMQETKPDEKIHIIYERGGKRKETDATLKSASGGAY
                     YFNFNNDEGYNNDIRIAPCLPTPPGSSYSYYSRDSSKTINICITGAYAGDYGDSTGGF
                     LPDQAHPLMDPNNLAVYANPSDGTFRIHVDLPEEGDTKVVITDMAGKEVYNELLPNFS
                     GTFDRTVTLSNASKGTYFVKVTQNGYSSTRTVVIQ"
     gene            complement(10986..11351)
                     /locus_tag="PLM90_09350"
     CDS             complement(10986..11351)
                     /locus_tag="PLM90_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ89620.1"
                     /translation="MKMKKATSVEGYIADFPKDIQLKLMEIRALIKKTAPKAEEGISY
                     GMPGYKLNGPLVYFGGFKQHIGFFPTGEGVAEFAEELKDYKTSKGTIQFPYDKPLPKT
                     LITRIVKHRIKVNEAKTKK"
     gene            11482..>11735
                     /locus_tag="PLM90_09355"
     CDS             11482..>11735
                     /locus_tag="PLM90_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ89621.1"
                     /translation="MKKKNIIYWVVTGIVAAYMVFSALPDALLQDKAVEFMQGLGYPD
                     YFTRFIGIAKLLGVAGILLPLPLRLKDWAYAGLVFDLVGA"
BASE COUNT         3321 a         2269 c         2568 g         3577 t
ORIGIN      
        1 atcctcaaac caggtgttgt cgtcgagcag gcgtctagtg gagccgagtc ccataccgag
       61 tccgaattcc gcacaagcct cagccaatcg cttgttgatg gttcctgcct gttcagtgcc
      121 cccggtcata ctgctgatcc acagtggcat tcccaacgtt ttacccaaga agctggtctt
      181 cagcgatacc tctgcccgat gaaaccccat aaccggctca taataaaacc tgcggtcgtt
      241 ctcagcggct gcagcctgac ttttaaaagc tagttcgata tggtcttgct tgcgcgatgc
      301 agaatttggg tcgttcacgg ttgtaaaggt aagcaaagta ggaattagcc aattagccga
      361 ttagccgatt gagtaatgtg agaaaaattg tgcaataaac tctgcggctc tgcgcctctg
      421 cgtgaaattt tggggattct aatgaaatga ataaacgtga ttcacttatt tgaattagta
      481 ttattgaaaa atctctttga attcttcaat catactgaaa cttttttctc ccaggttggt
      541 aaccgtaccc tcgataacag ttttcagttc gttgtaattt ttatgataga attgagaaag
      601 cttaaccact ttcttcccgt tggtcattag atataagtat tcatatttat cataattcga
      661 aggtaaaata gcagtttcaa acccttcgaa ttgtgtgagg ttgtacattc tttttttgcc
      721 tagtcccaaa taacttgaaa ccattacggc gtcatccttc aattcaacgc gaattacttt
      781 cgtccgcagt tctccaaaaa caatccatat caaaagaaaa ataaaaaaca gcatgaaagc
      841 aaaaattccg atcgaagggg taattgaacc atctatgaaa cttcctataa taaatcccaa
      901 tagtaccaat acaaaaagcg tcattgcgat tggcattgct atccggaatt tgaatttgga
      961 gtggaggtgc attaagattg gttaatgagt ttacaagttt gcgtgcagca ggctttttcc
     1021 attgatgttt attcgaagat acaaagtttc aaaaaccatc agaaaactga tctccaacac
     1081 agatagtctt cctgccgcga gatatgtggg acggagggga agttttaagc gcagaggtcc
     1141 agagtgcgga gttgtaaact tcagcattcc gtgagcgctt accccaattg gctaatcggc
     1201 acattggcta atcggctaat tggctaatta gctaattgat cgcctcctgt tatagtaaag
     1261 atctcatgcc atagcgtcca tgcaatccca tgtaccttat ctttgcccca tgaaattctc
     1321 ctcttttcca taccatcgtc ccgatatgtc tgcgttgcaa gcgcagtttg aagtggcgtt
     1381 gggaaaattt gaacatgcgg caagtgcgga aaatcagttg actgctatgc aggaaatcaa
     1441 tgccgtgcgc agtgcatttg atacaatgta tcagatctgt tctattcgtc actctattga
     1501 taccagagat acattttata cagaagaaca ggcatatttc gatgagcatg gaccggagat
     1561 ggaagcgctc atcagtaaat attatcaggt attagtgaaa gctcgttttc gtgaagcaat
     1621 tgaacagaaa tatggcaagc aattatttcg tatcgcggaa atgcagttgc gcgcatttca
     1681 tccggatatt atcccggatc tgatcaacga aaataaattg aatacggcat atcaggaatt
     1741 ggttgcatct gctttgattc catttgatgg tgctgaacga accctggctg aaatggggcc
     1801 atacatgaag agtgttgatc gcaacgtacg tgaacgtgct gcggctgcac gctggcaatt
     1861 ttttgaggat aacagagcga aatttgatac tattttcgac gacctggtga aaacgcgtca
     1921 tcgcattgca aaaacactgg gctttgataa ttttattgct gtcggctatc tgcgtatgca
     1981 acgcagtgat tataatgcag agatggtaga tgtatttcgc gagcaagtgc gtaaatatat
     2041 tgtgccgctg gctaccaacc ttcgcaatca gcaagcttta cgtcttggaa tatcgacatt
     2101 gaaatattac gacgaaggat tgcagtttac atcaggcaat gcaacaccaa aaggatcacc
     2161 ggaatttatt attggaaagg gtggcgaaat gtatcgtgaa atgtcgccgg aaacggcaaa
     2221 gttctttcat gaaatgcttg acaatgaatt aatggacctg gtggcaaaaa aaggaaaagc
     2281 cggtggtggc tattgtactt atatcagcgg acatcgcgcg ccgtttattt tttcgaattt
     2341 caatggtacg agtgccgata tagatgtgct tacacatgag gtgggacatg catttcaggt
     2401 atttatgagc aggggttttg atacgccgga atatttttgg ccaacgagtg atgctgctga
     2461 gatacatagt atgagcatgg aatattttgc ttatccgtgg atggatcagt tctttaatgg
     2521 tgaagcagat aaatatcgct atgcacattt ggttgagtcg ctgcagttct taccgtatgg
     2581 cgtggcagtt gatgaatttc aacatcgcat gtatgcagag ccggagctga cgccattgca
     2641 acgtaaaaat atatggcgcg agattgaaaa gaaatatttg ccgcatcgca attatgatgg
     2701 aaatatattt ttagaggaag gtggcttctg gcaaacgcag gcgcatattt atcagtcgcc
     2761 gttttattat atcgactata ccttagcgca gatgtgtgct tttcaatttt ggatgatggc
     2821 acaggaagat acaacaaaag cattcaacaa ttatgtggaa ttatgcaagg caggtggaag
     2881 caaatcattc ctggaattgg ttgcctatgc aaaactaaaa tctccatttg acccacaagc
     2941 cttcgcagat accatcggtc atatcgacaa ctacctgaaa ggggttgatg atttacagtt
     3001 gaattaactt ttttttcacg cagaggcgca gagcctaaac tgcatttctc cgcgtctctg
     3061 cgcctctgcg tgaaccaaac cgtctaaaat ccctattgca gactactaac cctttccaac
     3121 caccagaagt aattgaaatt attgtaaagt cttttttctt caggttgttg cgacaatgct
     3181 tgtaattgtt gatgaagttt ttgcttgagt ggttttagcg gtgagcgcat atgttgtttt
     3241 agtgcgcgca aaacagtggc ataggtagat gcttttaatt ttctggattc aagatggcgg
     3301 ttgaaactga cgattaggga atcgatgagt aggatattcc ctaattcaaa gtgacaaagt
     3361 atttgcatca ttctgctatc ggtgaacaag tcgattactg tttctgcatc cttcatatcc
     3421 attagcgttt gcagcgcatc aagacattgg tcgaattgtt tattacagaa tagaacgtag
     3481 gcgcataaat aatacaatgt aatcaaattc ggacgagcga taaatggtac aacatttgta
     3541 atttgagcaa gcacggcagt tgctacggat aatgcgtgca caaagtcttc taatccgacg
     3601 taataattca attctaataa ataactcatt ctgaatacct gggcatcaag atgttgcatg
     3661 tgccggaagg catcttcttc cggtagttta cgcaaggtct gtatacctgc aatcagcgct
     3721 tcatgttttt gaagaatgag gcagtcgatc agataattat tcaatgtagc aaaataccga
     3781 tcggcatatt gttgtcgcaa gtatggctga tcatccatca attgcaggaa ggcattatta
     3841 tagtcgaatg ccgtttccgt ttgttgttcg gcgaaagcgt ataatgccag cacctgcatg
     3901 cgatcgagac gcgcacggaa agtagtagct gagcttgcat ctgcaaacaa tggtgatgct
     3961 gcgagcgact tcaatgcgtg gttatcgcga cccggaccga gttttttctc gatatgcatt
     4021 ttatagatgg cagcagagtt tatccaatat tccgaggtaa tctttatttg ttcaagacaa
     4081 tgcaattctt cgcgatgtaa agctgcaagg ccgttgtgat tatagtcggt gtattgtgtg
     4141 cgtgcggcta accgtttttc tatcgcaata agcgcaatca cctcttcaaa tttttcaaag
     4201 gcatatccct ttgccttata tttctttatt tgcttagcac aagcagcata tagtccacga
     4261 tttaataaca aggagcaata atgcacgccg agcgttagtt tgtgcacttc atcatcatag
     4321 gcttcaaaac gatgtaatgc ctccagaagg gcatcgtaaa gttgtttttt cagcaccgga
     4381 aagtatgatg ccgacttacc ggtagcgaac tgttttgtta aagtttgttc ccggtacttg
     4441 tcctgagcat cgatagcttc ataaaggcga tggtaagtgc tatctttcgg gaaatgtcgg
     4501 gtggcaaaaa gcttgaaata gcgcttttca gccatcgaca tggattttat caggtcaaaa
     4561 agggaagtat cttgtttttt cacagacctc taatgaaatt tggcttgaaa tgcttgataa
     4621 tattgataca aataccaaaa atgtcctatc agacctctaa tataagaaag atatttgatt
     4681 tccgtggaaa caacggttgg cggagatttg tatccgtaaa cagaacgtta tgaagaaaat
     4741 gtataccatc atcgcggcga tactcctctc gaccgcagtt ttgcctctgg gtgctcaatc
     4801 gtgggtaaac tatagcaatc tcagctatgt taccgatgcc gagccgtctg gcacagatat
     4861 ctggatcact gctaaaggag gtgtgcagtt attcaatacc ctaaccggca cttcaatgtt
     4921 tttcagaaag ggtgatgcgg gactaccatc ctcatcagtt gaacaggttg ctaaaagcaa
     4981 tgccaccggt gtgatctggg tgggaactta tgatgccgga gtagttgagt gggatggcga
     5041 ttcgtggttg acatatccat ttccttctgc aatgttgctc tatcgcatga aattcgatgg
     5101 attcggaaat ctgtggttgc agactgatgc aggcttattt catttcaata ccactgatca
     5161 cgtttacact tttgtgaact caacaggtgg tgcaggatgg gattttaatg catgggattt
     5221 tgatatcacc cccgataata aagtaatcat atttacaggc accaattgtc aggtaataga
     5281 tgctgctact ttggcagcaa tcgattcatt ccccaattca gattcaccaa atgtgctggc
     5341 atgctcccct tcaaatgtgc gcgtgtataa tgtagatgaa gaaacgtatt taattaataa
     5401 cggcttcgcg atagaatttg aatttaaaga caacactttc actcccgcaa cagacggttt
     5461 gcctgaattc gctttcgtgc aaaatattat tcgcggaacc gatggagcat tatatgctta
     5521 tgtaaataat gcatctgttt atcgcctcga tggtctgaca tggacatatg ttgataatgc
     5581 agctgtttat ttccctgaat ttttagtgta taccgatggt gtggaatttt atttcagcag
     5641 taataattat gcatctactc ccggagtaat ccatatgtcg gaaacagatt atgtgaaaat
     5701 atcttcactg gaatatgcat ttacaagtaa tgccataaaa ggtttaactg aagatgcaga
     5761 cggcaatatc tttatactta gtggttcggg acaatatcaa tacaacgagc taaccgacga
     5821 tttttcgctg ttcggaaatg tgccttctat ttatggtact atttatgatc tccgttctgt
     5881 aaatggtata ttttattgcg tgaattatgg cgacctgctt gaatacaata acggtacttc
     5941 atgggtaaat attccttatg ccgacggata tacatcgcca tacatcttcg actatgatgt
     6001 tacttcagat ggtactatat attttgtaaa tgacgatggt gcattcaaat ataaagacgg
     6061 agaaacaacc tgtttgttcc caacaccatc attgtacgat tggggattat ctgtaaccta
     6121 tgatgccaca cgcaatttat tgtggatagg tcgcatggat ggtattttga aatacgactt
     6181 cgtaacacaa gaacttatcg atgccatgga tgtgccggca atgagtgatg gtatggccat
     6241 tcaggaaatt aaagtggcac caaatggtga cgtatggttc ggagcaaata ataataaagc
     6301 atatatgtat gatggcactg agtggactga ttacaatgtt ggcacgggta tgggatttgt
     6361 gacttcattt gattttgaag gagccaacat ttatttcggt atcaccgatg ggaatggtgg
     6421 tgtgcatgta tgggatacca gcaatgataa ttggtcatgg ttaagttctg caaccgatcc
     6481 aactttagta tctgatgaag tcagctacat gacagtaact acaggtggcg acttatggat
     6541 agcgcatgta aacgcaggtg taagcagatt ggacggcact ccatctgttg gtatagatga
     6601 ggttacagca aataatgcaa tcatttatcc aaatcctgtt gcgcagacat tatttgtatc
     6661 aggtttggaa gatggaaatt atcagtatgt aattagtgat ctttcaggaa aacaattatg
     6721 gtcatctagc caagcgactc attccattaa tgtatcgcaa tttccttccg gtatttattt
     6781 gttgcaagtg cgtgccggag ataaatcggt atttaacgga acatttattg tgccgaccaa
     6841 atagccacat caactcctca ataaaaaagc ccgatgtatt tctgcatcgg gcttttgttt
     6901 taatattatt aacccctaaa taaaccgtgt gaggaggttt gaggaattaa atgttcgcgg
     6961 tttcttcggc attcagtcga aatccgttgc catgaatatt tacgatttcg agcgttggat
     7021 catctttcag atatttgcgc aatttcgtta cgaaaacatc catgcttcgt gcggtaaaat
     7081 agtcatcgcg gccccagatt tgtttgaggg cttcttcgcg gtaaacgata tcatttactt
     7141 tcatgcaaaa cagtcgcaat aactccgcct ctttaggtga aagtttggcc tcgctatcac
     7201 catttttgag catgcgtgtt ttgtagttga aatgatattt tccaacattg aattctttct
     7261 gctgattgtt ggttctggct ttgcctccgc gttttaatac ggcggcaatg cgatagagca
     7321 gttcttcgct gttgaacggt ttggtgatgt aatcatcggc gccggttttg aatccttcca
     7381 gtacatcatc ctgtaatgtt ttcgcagtga ggaaaattac aggttgatcc ggattaacgc
     7441 gtttgatttg tgcggcgagt gaaaaaccgt cttttttggg catcatcacg tcgaggacgc
     7501 aaagattaaa ttcttcctta aaaaacagat cttctccttt ttcgccatcg gtggccagac
     7561 gtacttcgta accattcatg gtcaggtagt cttttagtac ggccccgaaa ttagggtcgt
     7621 cttccaccaa cagaattttg gttttgatct cttccatagt agtagtatgc ttgttttatt
     7681 gatgcggtaa aaaaatcctg aattgtgaac ctttctttaa ttggcttttc acctcaatgg
     7741 aacctttgtg tgcctcaaca acagttttca cataggttaa cccgaggcca aagcctttga
     7801 tattatgtac gtttccggtg gggactctgt aaaacttctc aaaaatcatg ctcatatcat
     7861 cattgctcat accaatgccg ttatcctcta cggtgatata aatgccctta ttgtcgctgc
     7921 gtgtgcgaac aacaatatgt ggatgttcag gcgtgtattt attggcattg tcgagcaggt
     7981 ttgagattac attactcaaa tgtacttcat cgccggtaat ttcaaatcta tcggcaagta
     8041 attctgtttc cacagtgccg cccttttctt ccacaagcag gctgatattt tccacagcac
     8101 gataaattat ctcatgaatg tcaacatcat cattgctgat acttacctga tgtttatcaa
     8161 gcagtgccat ttgcaatact ttttcgacct gtgcattcat acgtttattt tcatcgcgaa
     8221 taatgccggt atagtatttc accttttgct gatcttgcaa tatcataggg ttatttacgg
     8281 catccacggc taaagagatt gttgccaacg gagttttaaa ctcgtgggtc atattattga
     8341 tgaaatcatt tttgatttcc gacatttttt tctgttttac cagaataaat atcgtgtagg
     8401 tgaatacggc aacaataatg gtagtgaata ataaggaacc taacagcaag gaccagatac
     8461 tgctcaggat aaagcttttt tgatggggga agcttaccag cagaacatcg ttgttaaata
     8521 caatatcgtc ggggaacaga cttactttgt gcgatgatgc tgcaagtttc tcgaggccga
     8581 aattatcttc ggcattgctg ataaagaatt tatcatgcat gagtacacca aaattaaaat
     8641 cggtggtgat accgcgatta ttcagctcgt gatataaact gtttttaaga aaattcgtgt
     8701 cgattgtttg ctcgggagtg ataccacgtt gtaccatgcg tatcatcatt tgttccattg
     8761 ccttttttat gcgacctgaa tttaattgta attgcctgtc gatgtcggac aatatttgag
     8821 ataccagtgc atcattgggt gatgaattat cgccgattaa ttccagcggc tccagaatca
     8881 tggtaggctt ttgagaaccc ggcattcctt gcaaggcatc ggcaaaaggc tctattgttg
     8941 tatattccgg atcattataa tatgcctgat tggagcgaag cgattccagc atacctgcca
     9001 tggtgctgtc gctcaacaca ttttcataag tagtcaaaga atcgcctgtg cccatccagt
     9061 aggtggagtc ggaaaaaaac aaattcatct gactgctgag caccgaggct gcaatatttg
     9121 tctctacctt gtcggcaacc cgattaagtg cctcatttac atggtaattg aagatttgtt
     9181 ccttctgttc aatagtgttt tgaatccagt atacctgcac caggatgatg cccagcagcg
     9241 ccgtgctcat cgaaattaca atccaccata tccggtttct taacattcct tacaaaacta
     9301 catttgttaa aattgctgcg gcgacgttta acgatacatt aacatctttt atcaggctct
     9361 taaccccaac ggcacttgtt tcagattaca tttgtcatcg tatcgtccgg aaatgacccc
     9421 ggagcagtaa tacaacacaa accaatacaa gtatgcaacg gttccttttt agtatcacta
     9481 cgctcataat accggccatg ttgtttgcgc aacagcctga taatcagata catctgcgca
     9541 ttgatgccaa tgttgacggc cagcaggtta aaattgacac cttcatagaa tcgcttggcg
     9601 acctcaatct ggacgagttc ttacagggtt tgggactgga gaatgagctc aatcagctga
     9661 acattgacat caacagtggt tttggcgtca tgccgagttt tgatgaggag gctatgcagg
     9721 agatgttgaa gagcttgagt gagttggaaa tgcctgagat gcctgaatta cctgagttac
     9781 cgggaattga tatgggggaa gccatgtcgt ttgggtatgc aggagaaaac agagcatttt
     9841 tgggcgtata cactaataag gtaactgagg gtgcgcagat aagtgatatt gttgaaggaa
     9901 gtgctgcgga atctgcagga ttgatggaag gtgacatcat aacgcatatt gatgcaagaa
     9961 gtatagaatc accggcaaac cttatggaag tattagcctt gtattatccc ggtgatgaag
    10021 taacggtaac ttatatccgt gcaggtaaaa cagataaagt aaaacttacc ctacaggaaa
    10081 atacaaatgc atcaagcaca tggggctcgc agaactacaa ttttaatttt gatttccctg
    10141 attcaatttt tatgggccag ggtaattatt taatagaaac accggcgcat ggttatttag
    10201 gcgtttatct ggaagatgct gatggcaaag ttttggttac aggaattgaa ggcggatctg
    10261 ctgcagaaaa agccggtttg aaagctgatg atattcttct tgaaataaaa ggcaacaaag
    10321 tgcaatcgta tgatgaagtg ttagagatca tgcaagaaac aaaacctgat gagaaaattc
    10381 acattattta tgaacgcggt ggtaaacgca aagaaacaga tgcaacatta aaatcggcaa
    10441 gcggcggagc atattatttc aattttaata atgatgaagg atacaataat gatattcgca
    10501 tagcgccatg tttgccaacg ccacccggta gttcatacag ctattattca cgcgatagct
    10561 caaaaacaat aaatatttgc atcaccggag cgtatgcggg tgattatggc gatagcaccg
    10621 gaggattttt gccggatcag gcacatccac ttatggatcc taacaatctc gctgtatatg
    10681 ccaatccgag cgatggcact ttccgcatcc atgtagacct tcctgaggaa ggagatacaa
    10741 aagttgtcat tactgatatg gcagggaaag aagtttataa tgaattgctc ccgaatttct
    10801 caggcacttt tgatcgcaca gtcacattat cgaatgcgtc aaaaggcact tattttgtga
    10861 aggtgacgca gaacggttat tcaagtacaa gaactgtagt tattcaataa aaggtttaaa
    10921 ggtttaaaac aaaaagcgca tcaaaataat ggtgcgcttt ctttttgtga tcatttttcc
    10981 ggtaatcatt tttttgtttt cgcctcattc actttaatgc gatgttttac tatgcgcgtg
    11041 atgagtgttt ttggaagggg tttatcataa gggaattgaa tggtaccttt tgatgtctta
    11101 tagtctttta attcctctgc aaattctgca acaccttctc ccgttgggaa aaagccaata
    11161 tgttgtttga aacctccaaa ataaacaagc gggccattca atttatatcc gggcatgcca
    11221 tagctgatgc cttcctcggc tttaggtgct gtttttttta ttagcgcacg gatttccatg
    11281 agttttaatt ggatgtcctt aggaaaatcg gcaatgtagc cttcaacaga tgttgctttt
    11341 ttcattttca tgatagcaga ttatatatac aaatatacac gttgtattaa ttttcctgta
    11401 agcatttatt aaagtccaat gagaattttt caggtttaaa gtcagaatta tttacttttg
    11461 aacaggcaat caaagaaaaa catgaagaaa aaaaatatta tttattgggt agtaacgggg
    11521 atcgttgcag cttatatggt attcagcgca cttcctgatg cacttttgca ggacaaggcg
    11581 gttgaattta tgcaaggact gggttacccc gattatttta cccgcttcat cggcatcgct
    11641 aaattgttgg gagttgccgg aatattattg ccgcttccat tacgactaaa agattgggct
    11701 tatgcaggtt tggttttcga tctggttggg gcctt
//
LOCUS       DAOQOU010000100        11588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2869_length_11588_cov_6.042747, whole genome shotgun sequence.
ACCESSION   DAOQOU010000100 DAOQOU010000000
VERSION     DAOQOU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2869_length_11588_cov_6.042747"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2883
                     /locus_tag="PLM90_09360"
     CDS             <1..2883
                     /locus_tag="PLM90_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ89622.1"
                     /translation="NSIGSGTSGEFSINVYQSTPPPANDDCAGAFTLAVGTSCSYTSG
                     TSLTATSSAGEVAACTGTADDDVWYKFVATATNPVITVQSGAAYNAVLSVYSGTCGAL
                     ANIYCINATSTAGIETVNTTGLVIGQTYYIRIYHSAAGAGSGSFQVCVYNPVPSCTTY
                     ISPADAAVGIVGAGTTLQWNAATYATSYDVYFGTVTPPPFATNTASTSWSTGALAPLT
                     TYYWSIVPKNDLGDPGSCSIYSFTSVPECTGTPVGGAAASTAASVCGTGTSFTLSLTG
                     ASTDAGIAYQWQADAGAGYADIIGATSSTYSTTEAGTTSYQCVVTCTPSGFSATSTPV
                     SVTFNPAPTGDVLADPIIIASLPYTATGNNLSSNCWTNQIGQSSPDVYYRVIPECSGT
                     LNLNLCSGGTISDTYIHIADATGTILASNDDNGPSCAGLLSSINYAVSGGVTYYIIVE
                     GFSANEGTYTLNVSNSATYSVWYADADADLYGDPSVTTTTCDGFAPSGYVGNNLDCND
                     ADAAVNPSAIEVCNGYDDECDGLTDDADPDVIGFSVWFADADGDTYGNAAIIAEACAA
                     PVGYTADPSDCDDTNSAINPGATEICNGGIDDNCDGLADDADPGVVGAPSWYVDADGD
                     TWGDAATAVSACIAPGGYIADGSDCDDTQILYTDADGDGHGTATPAPCGATNTDDCDD
                     SQSTVYPGAPELCDGLDNDCDATIDEGTVTATVTPAVGAVTCKTYPYTFSANTGVGYT
                     YQWFKNGNLVVGATGPTLATDKPGYYQVQVNVPGGCFAMSDASLLTVNPLPNATISAP
                     NGTSLCAVVKLKVSENATWTYQWYLEGTPIAGATNFLYYPSVAGNYYAIVTSATGCFR
                     QSATLTVTSCRDAEVSGNETMNVYPNPTSSMFNIDLMMADGYNGTADVYVTNVLGDIV
                     LNKQLTVNNGAVSAELNLGVDAAAGMYLVKVVAGGKQFNQQLTVVK"
     gene            2956..4101
                     /gene="nhaA"
                     /locus_tag="PLM90_09365"
     CDS             2956..4101
                     /gene="nhaA"
                     /locus_tag="PLM90_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HMZ89623.1"
                     /translation="MKLTKLFTEFFESERTGGIILICCTFVSLLIANSALGTGYNAFW
                     HHEIAGHSIVHWINDLLMTFFFLLVGLEIEREIYIGELKEFRKASLPIFGAIGGMAIP
                     ALVYIGINISSDNMNGFGIPMATDIAFAIGILSLLGNRVPASLKIFLTALAIIDDLGA
                     IIVIAIFYSQSVSLLYLGAALTLFMIMIIMNRMKVMRLYLYLIPGILLWYCMLQSGIH
                     ATIAGVLIAFAIPFREGDNHSPSYKLQHFLHKPVAFFVLPIFALANTAIVLNTQLFSG
                     LQSTHALGIAFGLIFGKPIGILLMCYLAVKLKIAGLPEGVNWKQLAGVGLLAGIGFTM
                     SIFISLLAFEDDQMINISKLAILISSIIAAVTGLWYLSRQMDKQISA"
     gene            complement(4254..4682)
                     /locus_tag="PLM90_09370"
     CDS             complement(4254..4682)
                     /locus_tag="PLM90_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ89624.1"
                     /translation="MIYKRNASDFMTGNVIVAGVNNTFDQVMEFFTRHKIQHLPVAEG
                     TRLIGIISIKDMLTFMQDYLAGSGDVSKAALNAAFSIEKVMTANPTTVSPDTPQKDIL
                     EILAAGKFQAVPVVKSGDLVGIITNKDITRLYHYDATHIL"
     gene            complement(4822..6885)
                     /locus_tag="PLM90_09375"
     CDS             complement(4822..6885)
                     /locus_tag="PLM90_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89625.1"
                     /translation="MNKSIMTLLFGGVICFTAQAQGLQQVTGTSQQYSVNSADEPRMG
                     DSFVWNTEQFADLGILRYTIPDFDKLTLNQKLLVYYLTEAGYAGRDIIWDQNYKYNLT
                     IRKALEHIVENYTGDKQSKEWQDFMIYTKRVWFSNGIHHHYSTDKIMPGCSKAYFISL
                     VKTTGAQLDTKIIDIMFDPVVDNKKVYLQPDKDLLLNSASNFYADDVSEKEVEAYYAA
                     QPGMNDEKAISHGLNSRMIKTDKGLVEVVYKADGLYGDAIKQIIANLKLAMPHCENDM
                     QANALRLLIKYYETGDLKVWDEYNIAWLQDTHGDIDYINSFIEVYNDPMGYRGSFESI
                     VEIKDFEASERMQKLAEHAQWFEDYSPIMKSHKKDNVVGVSYRVVNVAGEAGDASPST
                     PIGVNLPNSNWIRADYGSKSVSLGNIVYAYEQAGNSSLLSEFCYTEEERQRAVNYGSL
                     AAKMHTAMHEVIGHASGKINPGVGTPKETLKNYANTLEEARADLVALYYTMDPQLVEW
                     GLIPSLEVGKAEYDSYIRNGLMLQLRRIEPGKTIEESHMRNRQMIAAWVFEKGKSKNV
                     IERKYENDKTYFVINDYEALRQLFGELLREVQRIKSEGDYEAGMKLVETFGVQVNEEI
                     HAEVLARTANIKTPPYWGFINPQLSIVKNDQGEVTDVTVSYPEDFTQQMLYYGKHYSY
                     LKGGQ"
     gene            6999..7298
                     /locus_tag="PLM90_09380"
     CDS             6999..7298
                     /locus_tag="PLM90_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89626.1"
                     /translation="MKKKLLLSILVVMAGTIAAVYAVNHRSEAPTQIAAPTEQVSAEN
                     PAHCDPSNCSPEEAAKCPYANKAAQATTESADNVCPATKECPPSKCNSASKTATL"
     gene            complement(7629..8225)
                     /locus_tag="PLM90_09385"
     CDS             complement(7629..8225)
                     /locus_tag="PLM90_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HMZ89627.1"
                     /translation="MEYVEHLSKDPVMAAMIKKHGAFTLKKQKDICTYLCYSIMSQQL
                     STKVADVFHARFDKLYDGKPTAEKIMATSIETFRGMGLSQAKANYVINVATFAQTQGM
                     EVKQLQAMDDMEIIKYLTQIKGVGRWTVEMLLMFALGRKDVFATDDLGIQQAMIGAYG
                     LRPRDKKKFRLRMEKIAEQWRPYRTYACMHLWRWKDNG"
     gene            8238..8642
                     /locus_tag="PLM90_09390"
     CDS             8238..8642
                     /locus_tag="PLM90_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89628.1"
                     /translation="MKIELFSSPDYFHTMSATESNTISSKLWFWSHFALVVIAWIGPF
                     IFDWYLMVTGYALVVIQFAVFNRCLMNDKHDLAEEGDMTFYAQLLESIGFNFSRAKVK
                     RIVRPWIYVFLAVFVIFLQVIFSYVPVLHIPG"
     gene            8695..10740
                     /locus_tag="PLM90_09395"
     CDS             8695..10740
                     /locus_tag="PLM90_09395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMZ89629.1"
                     /translation="MKKIFILGACGLFLSIAGAQGVMTPELLWSLGRVGAETVTPDGQ
                     HVIFGVTYYDIATGVGERNLFSIGLTDHKPIQLTTTKGGENSVMTLPGGKMGYIYQGQ
                     IWQSEWDGSNAKQLTNYPDGLSNVRFSPDGKHILFSKDVKLIDVLASEKYKDLPNSSV
                     RIYESLNYRHWDTWEDGAYSHIMLATWDNGIIGPEKDIMDGLPFDSPQMPFGGVEDFM
                     FHNDNDHIIYVTKALHGTDYTQSTNTDIYLYTMSTGDTKNITAGMEGYDTNPNFSPDG
                     NRLAWLSMARNGYEADKNRLYINDIKNKVVIDASESFDESIEALRWSNNGNFIYFLSP
                     INGTLQLFELDVTNVFTKVDGRKRNPIRQVTSGDYDVASIVGETNGMLVVTRTDMNHA
                     AELFLVNPSTGVMTAITEINKPNYDKIGMCKTERKMVKTTDGLDMLVWFIYPPNFDPN
                     KKYPTLLYCQGGPQSALTQFYSFRWNFQLMASQGYIVVAPNRRGMPGHGTKWNEQISK
                     DWGGQAINDYYSAIDYAKTLPYVDADRCGAVGASYGGYSVYMMAGTHQGRFKTFIAHD
                     GLFNLQSFYGTTDEMWFATWDLGGPYWSRGVQDNSYLQFNPITFVSEWNTPILIFQGG
                     KDYRTTEDQAFQAFQAAQLRGIRSRFVYLPDENHWVLKCHNGLAWQREFFDWLHQTL"
     gene            11071..11460
                     /locus_tag="PLM90_09400"
     CDS             11071..11460
                     /locus_tag="PLM90_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89630.1"
                     /translation="MIELKVHLFMNGGMSEELKAIIDTGAYYNYVCKRTVIGNSPVNY
                     IGDRTYFNINNVELTDKAYSAAIGIVGHKDPFKFEFMIKEDSFIDVILGTKFLSDFKI
                     ISILDGGVGKFKLYSSHFGDMISSLNI"
BASE COUNT         3187 a         2480 c         2366 g         3555 t
ORIGIN      
        1 aactcaatag gttccggaac aagcggtgaa ttttcaataa acgtttacca atctacacca
       61 cctccggcta acgacgattg cgcaggcgca ttcacattgg cagttggaac atcttgtagc
      121 tatacttcag gcacttcttt aactgctaca tcttctgcag gtgaagttgc tgcttgtacc
      181 ggcacagctg atgatgatgt ttggtataaa tttgttgcaa ctgctactaa tcctgttatc
      241 actgttcaat caggtgcagc ttacaatgct gtattatctg tttacagcgg aacttgtggt
      301 gcacttgcta atatttattg tatcaacgcc acttcaactg caggtattga aactgtaaat
      361 acaacaggac ttgtaattgg acagacatac tacatccgta tttatcatag cgcagccggt
      421 gctggttcag gatcattcca ggtatgtgtt tacaatccgg ttccttcttg tactacttat
      481 atcagccctg ctgatgcagc agttggtatt gttggtgcgg gaactacatt acaatggaat
      541 gcggcaacat atgctacaag ctatgatgtg tatttcggca ctgtaacgcc acctcctttt
      601 gctacaaata cagcatcaac atcttggtct acaggtgcac ttgcaccatt gactacttat
      661 tactggtcta tcgttcctaa aaacgatctc ggtgatccgg gttcatgctc aatttatagc
      721 ttcacatctg ttcctgaatg cacaggcact cctgtaggag gcgctgcagc ttcaaccgct
      781 gcttcagtgt gcggaaccgg aacttctttt acgttatcac taaccggtgc atcaactgat
      841 gcaggtattg cctaccaatg gcaagctgat gcaggtgcag gatatgctga tataatcggt
      901 gctacaagtt caacatattc aactactgaa gcaggtacta ccagctatca gtgtgtggtt
      961 acttgtactc catcaggctt ctctgcaact tcaactccgg tttcagttac atttaatccg
     1021 gctccaaccg gcgatgtgct tgctgatcca attatcatag cttcattgcc gtatactgca
     1081 acaggaaata acctgtcatc taactgttgg actaaccaaa tcggtcagtc ttcacctgat
     1141 gtgtattatc gtgtaattcc tgaatgtagc ggaactttga acttgaacct ttgttcaggt
     1201 ggtacgatct ctgataccta tattcatatc gcagatgcaa caggtactat cctcgcatct
     1261 aacgatgata acggtccatc atgtgcaggt ttattatcat caattaacta tgctgtttca
     1321 ggtggtgtaa cgtattacat tattgtagaa ggttttagtg caaatgaagg tacttataca
     1381 ttaaacgtat ctaattctgc gacatattct gtttggtatg cagatgcaga tgccgacttg
     1441 tatggtgatc cttctgtaac aacaacaact tgcgatggtt ttgcaccatc aggttatgtt
     1501 ggaaataacc tcgattgtaa cgatgctgat gcagcagtaa atccttctgc aattgaagta
     1561 tgtaacggtt atgacgatga gtgcgacggc ttgacagatg acgctgatcc tgatgtgatc
     1621 ggattctctg tatggtttgc tgatgcagat ggcgatactt atggtaacgc tgcaatcatt
     1681 gcagaagcat gtgccgctcc tgttggttat accgctgatc cttcagattg cgatgatact
     1741 aacagtgcaa tcaatccggg tgctaccgaa atttgtaacg gtggtatcga tgataactgc
     1801 gatggtttgg cagatgatgc tgatccagga gtagttggcg ctccttcatg gtatgtagat
     1861 gccgacggtg atacttgggg tgatgcagct acagctgtat ctgcatgtat tgctccggga
     1921 ggttatatag ccgatggatc tgattgcgac gatactcaaa tattgtatac tgatgccgat
     1981 ggcgatggtc acggaactgc tacacctgct ccatgcggtg caacaaatac cgacgattgc
     2041 gatgatagcc aaagcacagt atatccgggt gcaccagaat tgtgcgacgg tcttgataac
     2101 gattgcgatg ctactatcga cgaaggaaca gttactgcaa ccgtgacacc tgctgttggt
     2161 gctgtaacat gtaaaacata tccttataca ttcagcgcaa ataccggtgt tggctatact
     2221 tatcagtggt ttaaaaatgg taaccttgta gttggtgcta caggtccaac cttagctact
     2281 gataaaccgg gttactatca agtacaagta aacgttcccg gcggatgttt tgcaatgtct
     2341 gacgccagct tgctgaccgt taacccattg ccgaacgcta ctatcagcgc gccaaatggc
     2401 actagccttt gtgcagttgt gaaattgaaa gtaagcgaaa atgcaacatg gacttaccaa
     2461 tggtatttag aaggtactcc gatagccggc gctaccaact tcttatacta tccatctgtt
     2521 gcaggtaact actatgcaat tgtaacaagt gctacaggtt gtttcagaca atcagctact
     2581 ttaacagtga cttcatgtcg cgatgctgaa gtttccggaa atgaaacaat gaatgtatat
     2641 ccaaatccaa cttcatctat gtttaacatt gatttgatga tggctgatgg atataatggt
     2701 actgctgatg tttacgttac aaacgtgctt ggcgatatcg ttctcaacaa acaactcact
     2761 gtaaacaatg gtgccgtttc tgccgaactc aaccttggtg ttgatgcagc tgcaggtatg
     2821 tatcttgtta aggtggttgc aggtggaaaa caattcaacc aacaactcac agtagtgaaa
     2881 taattcactc ttacccttca ataaaaaacg accattggga aaccgatggt cgtttttttt
     2941 gttattattg ccaatatgaa gctgacaaaa ttgtttaccg aattctttga aagtgagcgt
     3001 acaggcggta tcattctgat ttgctgcaca tttgtttcat tgctcatagc taattctgca
     3061 ctcggaaccg gctataatgc tttttggcat catgaaattg cgggccattc tattgtacat
     3121 tggataaatg acctgcttat gacatttttc tttctactgg tgggattgga aattgaacgt
     3181 gaaatatata tcggcgaact caaagagttt cgaaaggctt cacttccaat atttggcgca
     3241 atagggggga tggcaatacc ggcgctggtg tatatcggta ttaatatttc ttctgataat
     3301 atgaatggat tcgggatccc gatggctact gatattgcat ttgccattgg aattttatca
     3361 ttgctcggaa atcgggtgcc ggcatcctta aaaatattcc ttaccgccct ggcaattatc
     3421 gatgatctcg gagcaattat tgttattgct attttttact ctcaatccgt gagtctgctt
     3481 tatttaggag ctgcacttac cctatttatg ataatgatca ttatgaacag gatgaaagta
     3541 atgcgcctgt atttatatct aataccggga atattgttat ggtactgtat gctccagagt
     3601 ggcattcacg caactattgc aggtgtgttg atcgcttttg ctattccctt cagagaaggc
     3661 gataatcatt ctccaagcta taaactgcaa cattttttgc acaagccggt agcgtttttt
     3721 gtgcttccga tttttgcact tgcaaacaca gcgatagtat taaatacaca attgttttcc
     3781 ggccttcaaa gtacacatgc attgggtatt gctttcggtt taatttttgg aaaacctatt
     3841 ggcatattgt tgatgtgcta tttagcagtc aaactaaaaa ttgcaggatt acccgaaggt
     3901 gtgaattgga aacaattagc aggtgttggt ttactcgcag gtattggatt tacaatgagt
     3961 atatttatca gcctcctggc atttgaagat gatcaaatga tcaatatatc gaaactggcg
     4021 attttaatat cttctatcat tgccgcagtt acaggattat ggtatttgag caggcaaatg
     4081 gataaacaaa tttctgcata aaaaaaacag caaccatccc tttcgagacg cttgctgctt
     4141 gtttatccct taaaaacagg tcaaatatac atcgaccggt caaattttgc aacttctaaa
     4201 gcaatatttt taagcgctta gaagcgattt aacattgaag tttttcctct caattaaagg
     4261 atatgcgttg catcgtaatg gtataagcgg gtaatatcct tgttggttat aatgcctacc
     4321 aaatcgccac ttttaactac aggaactgct tgaaatttgc cggctgccaa tatttccaga
     4381 atgtcttttt gtggggtatc gggggataca gtggtagggt tggcagtcat gaccttttcg
     4441 atggagaatg cggcatttaa agcggcttta gaaacatcac cggaaccggc aagataatct
     4501 tgcatgaaag taagcatatc ctttatgctg atgatgccaa tcaatcgtgt cccttcagca
     4561 acaggtagat gctggatctt gtgccgggtg aaaaattcca ttacctgatc gaatgtgtta
     4621 ttcacaccgg ctacgatcac attgccggtc ataaagtctg aagcgttgcg tttatagatc
     4681 ataagataaa aatttactct gcaaacctac taatgtgtca tatggctaaa aatgattttc
     4741 gtcaccgttt aaggtgcctt acgtcaaaaa aaaagcggca tctcaaatga aatgccgctt
     4801 aatttgttgc ttgatgatat cttactgccc gcctttaaga tagctatagt gctttccgta
     4861 atacagcatt tgttgcgtga agtcttcagg atagcttacc gttacatcag ttacctctcc
     4921 ctgatcgttt ttcactatcg acaactgcgg attgataaag ccccaatagg gtggtgtttt
     4981 aatattagca gtgcgtgcca atacttcggc atgtatttct tcattcacct gaacaccgaa
     5041 agtttctacc aatttcattc ctgcttcgta atcgccttcg cttttaatgc gttgcacctc
     5101 tctcaataat tctccgaata attggcgtaa tgcttcgtaa tcattgatca caaaataggt
     5161 tttatcattt tcatatttgc gttcaatcac atttttcgac ttcccttttt caaaaaccca
     5221 ggctgcaatc atctgacgat tgcgcatatg cgattcttca attgttttgc cgggctcaat
     5281 acggcgaagt tgtaacatca aaccattgcg gatatagctg tcgtattctg ctttccctac
     5341 ttccagtgaa ggaatcaatc cccattcaac taattgagga tccatggtgt aatacaatgc
     5401 aacaaggtcc gctctcgctt cctctaaagt gttggcataa ttcttcagtg tttctttagg
     5461 tgttccaacc ccgggattga tttttccgct cgcatgaccg ataacctcat gcatcgctgt
     5521 atgcattttt gctgccaatg aaccgtaatt taccgcgcgt tgtctttcct cttcggtata
     5581 acaaaattca ctgagtaaac ttgaatttcc cgcttgctca tatgcataaa caatatttcc
     5641 caagctcact gatttgctgc cgtaatctgc gcgtatccaa tttgaattcg gcaagttcac
     5701 accaataggc gttgatggcg atgcatctcc tgcttcaccg gcaacattga caactcggta
     5761 ggaaactcct actacgttgt cttttttatg cgatttcatt atcggtgaat aatcctcaaa
     5821 ccattgcgca tgctcagcta atttttgcat gcgttctgaa gcttcgaaat ccttaatctc
     5881 tacaatgctc tcaaatgagc cgcgataccc catcgggtca ttgtatacct caatgaaact
     5941 gttgatgtaa tcaatgtctc cgtgcgtatc ctgcaaccag gcaatgttat attcatccca
     6001 aacttttaaa tcgccggttt cataatattt gatcagtaat cgcaatgcat ttgcctgcat
     6061 gtcattttca caatgtggca tggcgagttt taagtttgcg atgatctgct taatggcatc
     6121 accatacaat ccatctgctt tatacaccac ttcaaccaat cctttatctg tcttgatcat
     6181 gcgactgttc aatccatgag aaatcgcctt ttcatcgttc atgccgggct gcgcagcata
     6241 atacgcctct acttcttttt ctgatacatc atctgcataa aagttcgaag ctgaattcaa
     6301 tagtaaatcc ttatcaggtt gcagatatac ctttttgtta tctacaacag gatcaaacat
     6361 gatgtcgatt attttagtat ctaattgagc acctgttgtt tttacaagcg aaataaaata
     6421 tgcctttgaa catccgggca taatcttgtc ggtagaataa tgatgatgaa tgccattgct
     6481 gaaccaaaca cgtttcgtgt aaatcataaa atcctgccat tctttcgatt gcttatcacc
     6541 tgtataattc tctacaatat gctctaatgc tttgcgaatg gtaaggttgt atttatagtt
     6601 ttgatcccag ataatatccc ttcccgcata acctgcttcg gtcaaatagt agactaacag
     6661 cttttggtta agtgttaatt tatcaaaatc aggaattgta tatcttagta tgcccaaatc
     6721 agcaaattgt tctgtattcc aaacaaagct gtcacccatt ctaggttcat cagcgctatt
     6781 cacggaatat tgctgactcg ttcctgtaac ttgttgtaat ccttgtgcct gtgcagtgaa
     6841 gcatataaca cctccaaaca gcaaggtcat gattgattta ttcatttgat tagtgttgag
     6901 aatacaaaat tgcataaatc gggccgaatt tcatatatcc acagcccgat acttgcatgt
     6961 taatatgtgg catagtatat ttacataaaa ttacgcgtat gaagaagaaa cttttgcttt
     7021 cgattttagt agtaatggca ggaactatcg ctgctgttta tgctgtaaat catcgttctg
     7081 aagctccaac acagattgct gctccaacag aacaagtaag tgctgaaaat cctgcacatt
     7141 gcgatccttc taactgctct cctgaagaag cagcaaaatg tccatatgct aacaaagcag
     7201 cacaggcaac tacagaatct gctgataatg tatgccctgc tactaaagaa tgcccaccgt
     7261 cgaaatgtaa ttccgcctca aaaacggcta ccttataatc tcttaaaaac agatttccaa
     7321 tggcaaagaa accacggcga ccaccgtggt tttttgtttg tacatatctg cactaacctt
     7381 tatcacccgt tttgggaatg ttaatcattc tttaaacggt aattccgata tctatttgta
     7441 tcgtaaatca cgatgtcatg cacaacgaac atgataacgt gacatgaata aaatgtgata
     7501 atccggtggt cgatatgttt gtatgatcac ctcaaagatg gttacagatg ttaaatgcca
     7561 tctgtttgcc tgttatatgt ttcatgggtt ttgaatgaac gccgatagta atgtcggcgt
     7621 ttattttttc atccattatc cttccatcgc cacaaatgca tacacgcata agtgcgatat
     7681 ggacgccact gctcggcaat tttttccatg cgcaatcgaa atttcttttt gtctctgggc
     7741 cgcaatccat aagcaccaat cattgcttgt tgaatgccaa ggtcatccgt tgcaaataca
     7801 tcttttcttc ccaaggcaaa catcaataac atttctacag tccatctgcc aacacctttt
     7861 atttgagtga gatatttgat gatctccata tcatccatcg cctgtaattg cttaacctcc
     7921 atgccttgcg tttgtgcaaa ggtggctacg ttgatcacat agttagcctt tgcctgactt
     7981 aaacccattc cccgaaatgt ttcaatggac gttgccatga ttttttctgc cgtaggtttg
     8041 ccgtcatata acttatcgaa acgcgcatga aatacatcgg ctaccttggt ggatagttgc
     8101 tgactcatga tagagtaaca caaataggtg cagatatctt tttgcttctt caaagtaaag
     8161 gctccatgct ttttaatcat cgccgccatt accgggtcct tcgataaatg ttctacatat
     8221 tccatgcggt aaatatcatg aaaattgaat tattcagcag ccctgactat tttcacacca
     8281 tgtctgccac agagagtaat accatcagtt ctaaattgtg gttctggtcg cattttgccc
     8341 ttgtagtaat tgcctggatc ggacctttta ttttcgattg gtatctgatg gtaacaggat
     8401 atgcgctggt ggtaattcaa tttgccgtgt tcaaccgctg cctgatgaat gacaaacacg
     8461 acctggcgga ggaaggcgac atgacgtttt atgcccaact cctcgaatct ataggattta
     8521 acttctcaag agcaaaagtt aagcgtattg tccgtccgtg gatatatgtt tttctggctg
     8581 ttttcgtcat cttcctccaa gtgattttca gctatgtccc cgttttacat attccgggat
     8641 aaatccgcat cacaacacca taatctctct tatcttcgca gcaaataaac tgttatgaag
     8701 aagatcttca ttttgggagc atgcggattg ttcctttcaa tagccggtgc ccaaggcgtc
     8761 atgacacccg aattattgtg gagcctggga agggtaggag cggaaactgt tactcccgat
     8821 ggtcaacatg taatattcgg cgtgacatat tacgacatcg ccacaggggt aggggagcgc
     8881 aacctgttta gtataggcct taccgaccat aaacccattc aactgaccac tacaaaaggc
     8941 ggtgaaaata gtgtgatgac acttccggga ggtaaaatgg gctatatcta tcaggggcaa
     9001 atatggcagt cggaatggga tggctctaat gccaagcaac ttactaatta tcctgatggc
     9061 ttgtcaaatg tgcggttttc tcctgatgga aagcatatct tgttttcaaa ggatgtaaaa
     9121 ctgatcgatg tactggccag cgaaaaatat aaggatctgc caaacagttc agttagaata
     9181 tatgaatcgc tcaactatcg tcactgggat acatgggagg atggcgctta cagtcacatc
     9241 atgcttgcca cctgggataa tggcatcata ggtcctgaaa aagatatcat ggatggattg
     9301 ccgttcgata gcccgcaaat gccttttggc ggtgtggaag acttcatgtt tcacaacgat
     9361 aacgatcata tcatctatgt gacgaaagca ttacacggca ccgattacac ccagagcacc
     9421 aatacggata tctacctcta taccatgtct accggcgata ccaagaatat tacagccggc
     9481 atggaaggct atgatacaaa tccaaatttt tcacccgatg gcaatcgcct ggcttggctg
     9541 agtatggctc gaaatggtta cgaggctgac aaaaaccgac tttatatcaa cgatattaaa
     9601 aataaggtgg tgatcgatgc ttcagaatca tttgatgaat ctattgaagc tcttcgctgg
     9661 agcaataatg gtaattttat ctacttcctc agccctatca acggaacttt acagttgttt
     9721 gagctcgatg taacaaacgt tttcactaag gttgatggtc gcaaacgcaa tcccatccgc
     9781 caggtaactt ccggcgatta tgacgtggcc agcatcgttg gcgaaactaa tggcatgctc
     9841 gtggtaacga gaaccgacat gaatcatgcg gccgaattgt tccttgtgaa cccctccacc
     9901 ggcgttatga ccgcaatcac ggagatcaac aaaccgaatt acgataaaat cggtatgtgc
     9961 aaaaccgagc gcaaaatggt aaaaaccacc gacggactcg atatgcttgt ttggttcatt
    10021 tatcctccga atttcgatcc taataagaag tatccaacct tattatactg tcaaggcgga
    10081 ccacaaagtg cactcacgca attttacagt ttcagatgga actttcagtt gatggcttct
    10141 cagggatata ttgtggtggc accaaatcgc cgcggtatgc ccggacatgg cactaaatgg
    10201 aatgaacaaa tcagtaaaga ttggggcgga caggcaatta atgactatta cagcgcaata
    10261 gattacgcta aaacgcttcc ttatgtggat gctgatcgct gtggggctgt tggggcgagt
    10321 tatggtggct atagcgtgta tatgatggcc ggaacgcatc aaggcagatt taagacattt
    10381 atagcccatg acggactgtt caaccttcag tcgttctacg gtacaactga tgaaatgtgg
    10441 tttgctacct gggatctcgg tggcccgtat tggtcgcgcg gagtgcagga caactcatat
    10501 ttgcagttta acccgatcac ctttgtatct gaatggaata cgccaattct catcttccag
    10561 ggaggcaaag attatcgtac aaccgaggat caggctttcc aggcatttca ggcggcgcaa
    10621 ttacgcggta tccgatctcg gtttgtttac ctgcctgatg agaaccactg ggtgctgaaa
    10681 tgtcataacg gactggcatg gcaacgcgag tttttcgatt ggttgcatca aactttataa
    10741 gctgaatctt ccaataaatc ataacaaaac acccggtcat tttgatcggg tgtttttgtt
    10801 ttaggagctg cctcatcttg tttgcagtaa catcgtatag gttcgcaagc gctgataaac
    10861 atggctattg tgcaatatgc taaaacgagc tcaaaccgtt gatatggctg aattccgact
    10921 ctattcttgt attaccagtt tttgcccata attaatatta tgttaagtcg gcgcgacagg
    10981 cttcccgctc cgcgctaaca ccaccttgca gttctcacag acatttggta ggtttggggt
    11041 atgatacaag gattattaaa tatccaccat atgattgaat taaaagttca tctatttatg
    11101 aatggcggaa tgtctgaaga actaaaagca attatagata ctggagcata ctataattat
    11161 gtatgcaaaa gaaccgtaat cgggaattcg cccgtaaatt acattggaga tagaacatat
    11221 ttcaacatta acaatgtcga acttaccgac aaggcatact ctgccgcaat aggtattgtg
    11281 ggtcataaag acccgtttaa atttgaattt atgattaagg aagattcttt tatagatgtt
    11341 attttaggaa ctaaattcct atctgatttt aagataatat ctatattaga tggaggagtt
    11401 ggtaaattta aattatattc ttcgcatttt ggtgatatga tttcatcgct aaacatataa
    11461 gcactttcta cgcctccgtt ccgctcgcta ctggtggctc gctccactcc gtgacctctg
    11521 aacttgccac acgacagcac gctccgcagc acatagctaa ccgacattaa cataatatca
    11581 gttatacg
//
LOCUS       DAOQOU010000101        11217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2987_length_11217_cov_4.600161, whole genome shotgun sequence.
ACCESSION   DAOQOU010000101 DAOQOU010000000
VERSION     DAOQOU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2987_length_11217_cov_4.600161"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLM90_09405"
     CDS             <1..625
                     /locus_tag="PLM90_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89631.1"
                     /translation="IFTIDYSNQTIYNKMKKVSLLLVMATTLLFTNSFAQVTQKGGTS
                     TDKPATTAEKPSSDKKDKVQSEMSGDAASTAKEWTMKMDEICNLDQAQEKKVMEINLR
                     YANKLEELKAKYKGMENPNADAAKAEKDELTKARFKEYSNVLSKEQMQKFKEYRNSKK
                     DENKGDSAGKSEKKDDMKEKYKNATPEEKEKMKEEMKDKKDKKQNKE"
     gene            complement(794..1807)
                     /locus_tag="PLM90_09410"
     CDS             complement(794..1807)
                     /locus_tag="PLM90_09410"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ89632.1"
                     /translation="MYQVTQHIVKLLYEHNCVIVPGLGGFVTNYQPAKVHPTSFIFNS
                     PGKSVAFNVSLSTNDGLLADTIARQEGISHRDADKLIVDFVSQIKTALSESKPVKLSG
                     IGRLTMDVEGQIRFLPDNTQNFQLQSYGLYSFTAQPITRDRQQIEVQTAKPVQAIEVP
                     ARKKRKFSDALLPIAIVLLICMGAMQVFIQKDMNGFNYSEIFGLSKLFPHNDYALKRY
                     ETDVYQINSSITYFRRTMPAPADTTVADSSSLTTSPAAVNTQPVSTHATYFVSAGSFY
                     SSSQAQKVVDKIAAKGYTGYVKPYGKYQVAAVDIPDNISQSAFRKAFIEATGIKGAWI
                     GKN"
     gene            complement(1878..3488)
                     /locus_tag="PLM90_09415"
     CDS             complement(1878..3488)
                     /locus_tag="PLM90_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89633.1"
                     /translation="MKKFAIYLAFTGLISSQAIAQIVGDEDVDVVSGYNPVLADAVKE
                     TFEASLPKQDIKPDPQTYNIPIEFFQIPYQPVKVKPIRMPDVKPEELDNVFVKAGFGT
                     QLTPLAEAYLNSNRSKKYNYGLYGKYISSNSKIDFQNYSDTRVGGTAKFFFDDKYVLP
                     VNAYFSTNSLNYYGFDHEVDTTYTDADLKQRFTNYGFDLGFANTGDNPLNMDFGLKAG
                     LKGISDINKYKEINPFLNAWGEKKLENNSIAGASASYEYYQYTGFGDYYNSLVGIKPY
                     YKMITDQYSLTVGLDSRMDKDQNAYILPDAVFSYDLIGDKFVFVAGWQGRTQVNSFAN
                     IVTENPFVADSLIFKISEIHEKFAGLRGSTSGNFSFSVKGYQKSAQDLPFYVNDTTDM
                     KRFLVKYSDATIWGGALEMSYFDSDRFRVTGSVNAFKFSEVADLDQPWHRPTLEWTLS
                     GMYRFNKRMSMTADVFGVNKTLALLPGDITEEVKGCVDMNISATYVYSKYFNIFVNLN
                     NLANYKYQRYYNYPSYGIQAMAGLSFAF"
     gene            complement(3509..6610)
                     /locus_tag="PLM90_09420"
     CDS             complement(3509..6610)
                     /locus_tag="PLM90_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89634.1"
                     /translation="MIRNKLIIACMCIVLCGTTGALKAQQSLIYTDLYANYKKAMELY
                     ENKMYATAQEYFEQVGSVTPEGTDGDIFTYVSNSRYYAAICAIELNNTDAEKMMLDFV
                     HNNPGNVMLGSAYNQLGKLYYNKKEYTNAATWFEKVDVKDLSLEERDTYKFQYGYSLF
                     NKKRFTEAKVMFASLKDKPQNQYYNAANYYYGFIQQEDGNYENALTCFNIVKDVPPYN
                     AVVPYYITNIYFQQGKYEKVISYAEPLMADTKLVYFAEINSLIGQAYFYRNNYRKALP
                     YLNYYIEKAKNIRDEDYYQLAFAQYSTGDSKAALTTLKETSDDNDTLYQHIQYLYGNC
                     YIALDKKEEARNAFMEASRIGEDKTIHEQALFNYGKLSYESGYHTAAVTAMQDYLKAY
                     PKGSNKTEAQELLANALLGTNDYLAAMDVIENIPQKTPKLKEAYQKVSYYSGVQLFNQ
                     KNFDEADKMFMQSLENPIDVNLQAACYFWIGEIRFAENKYALTITNHVKFQDLAKVAK
                     DLPSETRVPYSDYTIGYAYFKQSNYSNAVNYFSKVTTALKTSKTDMVQNNVYIDASLR
                     LGDCYFMTKDYKKAETNYNKIIDNNAKGVDYAMYQKAMLQGLQNNSNGKIATLQSLTN
                     KYPNSLYVDDAMYEIANTYFITKKNTESIAAFKELVSEKPNSSYTIKAYLKLGLIYFN
                     EKDYKNSEKYYRLAYESSPNSEEGQQAKEGLADIVKETGNTGAADGVVEVTKIDEEKY
                     NYAKNKYDLNEFSGAAIAFSDYLREFPKGGYKSEAEFYRGESYYQLKQYAKALPDFES
                     IIKGNKTKFLESALVRAGWIEYYQNEDYQTAYNYYERLYEVADFKENTFVAMVGLLQT
                     AYFLNDFDAVIVNANRILASDLVTNKERIDAHYYSGQAHLANNNIDKAYDAFSKAAAL
                     TTNEIGVESRFHMADILFQKGKLEESKTKCNEIINNMPSYDEWIIRSYILLADIAAAK
                     GDYAQAKATLNSIISGYKGDPELVELAKQKLEQIEEMEKSGRRKSPASDTNDESEINF
                     DNN"
     gene            complement(6785..7255)
                     /locus_tag="PLM90_09425"
     CDS             complement(6785..7255)
                     /locus_tag="PLM90_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89635.1"
                     /translation="MRLRLIFPILLLAIVSCNEPERGTLDPETMSKKLFDAMKSNSPE
                     KIALLLPDKGTFRKIEEENGHILVNLEAGYNGFEKQSSACLDTVRNKVSNWSSATFTR
                     GNYTESKLGKLPKARVTTKFDADGSPRKVEFTAVKFNNRWYYYGDMTWIDKIVE"
     gene            7360..7833
                     /gene="rnhA"
                     /locus_tag="PLM90_09430"
     CDS             7360..7833
                     /gene="rnhA"
                     /locus_tag="PLM90_09430"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HMZ89636.1"
                     /translation="MVQIYTDGSARGNPGPGGFGTILRYGKHEKELSGGYRRTTNNRM
                     ELMAVIAGLEALTKEGLVITIYTDSKYVCEAIEKGWIYGWMKKGFAGKKNADLWTRLM
                     RAYARHKVKLIWIKGHAGHPENERCDVLATTAADGHKLAIDEVYEMENQTKLGFD"
     gene            complement(7877..8890)
                     /gene="fbp"
                     /locus_tag="PLM90_09435"
     CDS             complement(7877..8890)
                     /gene="fbp"
                     /locus_tag="PLM90_09435"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMZ89637.1"
                     /translation="MKVKTLIEFIVERQQDFPFATGELTRLLSDISIAGKIVNREVNK
                     AGLVDILGAEGSENMSGETQQKLDVYANNQFMNALMAGGEVCGFASEEEDNFISFDGA
                     ISKSGKYVVLIDPLDGSGNIDVNVSIGTIFSIYRRLSLSGGATLEDFLQKGTEQVAAG
                     YIIYGSSTMMVYTTGFGVNGFTLDPSIGEFCLSHPDIRIPNRGIIYSINEGNYVHFPE
                     GVKRYIKYTQEEDLKSQRPYTSRYIGSLVADFHRNMLKGGVYLYPETAKSPKGKLRLL
                     YECNPLAFIAEQAGGVATNGRKRILDIEPKELHQKTPFYIGSSAMVEKIVEFLDRFTE
                     AKV"
     gene            8971..9423
                     /locus_tag="PLM90_09440"
     CDS             8971..9423
                     /locus_tag="PLM90_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ89638.1"
                     /translation="MEYHIRPADGADIAMIHRLVSQLAEYERAADQVITTTADYQRDF
                     EAGRFQALVAVDLQSGEIVGMALYYFAYSTWKGRYLWLEDFIVTEQCRGKGVGRLLFD
                     AVGAIAAKENTFMKWQVLDWNTPAINFYNKYPMEVQDEWLTCRMPVRS"
     gene            complement(9451..9813)
                     /locus_tag="PLM90_09445"
     CDS             complement(9451..9813)
                     /locus_tag="PLM90_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89639.1"
                     /translation="MRYHSIVQLACWMMCHLWFIVFFTRPNIHPDWWEIMIFGSIVQI
                     MGIIIYAINKEFRFAWMILLAMPVIWIAGMYTDNADLDLSDLGEVFILWMFRIIIIHV
                     LYFLTIIPILRDVLYETE"
     gene            complement(10115..10504)
                     /locus_tag="PLM90_09450"
     CDS             complement(10115..10504)
                     /locus_tag="PLM90_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89640.1"
                     /translation="MRHFFSNNASRAAIVFLPVILFLIACDPGSCITFKIANESNGVV
                     DVTYFEYDSNKVTQIRIPEHTEQILFQQCFLGYVDRFENQDSVDLSYHVRIWKDSVLL
                     GTNLNDKQSWDYIRTNKSEAALTLRIK"
     gene            complement(10527..10892)
                     /locus_tag="PLM90_09455"
     CDS             complement(10527..10892)
                     /locus_tag="PLM90_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HMZ89641.1"
                     /translation="MATTKSHEARIAAMTFASVYPLYLAKIEKKGRTKDELHQVIEWL
                     TGYNLVTLQKLIEKRVNFADFFDKAKLNPNAKLITGMICGYRIEEIQDPLTRKVRYLD
                     KLVDELAKGRAIEKILRKE"
     gene            complement(11032..>11217)
                     /locus_tag="PLM90_09460"
     CDS             complement(11032..>11217)
                     /locus_tag="PLM90_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89642.1"
                     /translation="ACPEVTKCAAKRSIFSTSGYDYDNHIMKGDFSEPRRYKMFCTLQ
                     KTLNRYQDARKAMLVFI"
BASE COUNT         3238 a         2270 c         2315 g         3394 t
ORIGIN      
        1 gatatttaca atagattatt caaaccaaac aatttacaac aaaatgaaaa aggtaagttt
       61 actcctggtg atggcaacca ccttactgtt tactaacagt ttcgcccagg tgactcaaaa
      121 aggtggcact agcactgata aacctgctac aactgcagaa aaaccatctt ctgacaagaa
      181 agacaaagtg cagagtgaaa tgtccggcga tgcagcttct actgcaaaag aatggaccat
      241 gaaaatggat gaaatctgta atctggatca ggcccaggag aaaaaagtta tggaaatcaa
      301 ccttcgctat gccaacaaac ttgaagagct gaaagcaaaa tacaaaggca tggaaaatcc
      361 gaatgctgat gctgccaaag ctgaaaaaga tgaattgact aaagcgcgtt tcaaagaata
      421 cagcaatgtt ctttcaaaag agcaaatgca gaaattcaaa gaataccgca atagcaaaaa
      481 agatgaaaac aaaggcgaca gcgcaggtaa atctgagaag aaagatgaca tgaaagagaa
      541 gtataaaaat gctactcctg aagaaaaaga gaaaatgaaa gaagagatga aagacaagaa
      601 ggataaaaaa caaaacaaag aataatatcc ttctgaaaga taagaaggcc tcctaaacgg
      661 gggctttttt tatgtacatt actccagatg tccaggcaca attgcaggta tattcaatat
      721 gttattaaat gccaatttct ccccttttaa aagcgatgga tatccaattc aaggccaatt
      781 aagctatgca attttagttc ttacctatcc aggcgccctt aatgcctgtt gcctctataa
      841 aggccttgcg aaacgctgat tgagagatat tatcagggat atcaactgct gccacctgat
      901 atttgccata aggttttaca taacctgtat agcctttcgc tgcaatcttg tcgaccacct
      961 tttgtgcctg cgacgagcta tagaatgagc ctgctgatac aaaatacgtt gcatgagtgg
     1021 atacgggctg tgtgtttacc gctgcaggtg aagtagttaa acttgatgaa tctgccacag
     1081 ttgtatctgc cggagcgggc atggttctgc ggaagtaggt gattgatgag ttaatttgat
     1141 aaacatctgt ttcgtatcgc ttcaacgcat agtcattgtg cggaaacagt ttgcttagtc
     1201 caaaaatttc cgaatagttg aaaccattca tatccttctg aataaaaacc tgcattgccc
     1261 ccatgcaaat taaaagcact attgcaatag gtaagagggc atcagaaaac ttgcgttttt
     1321 tccgggccgg aacttcaata gcctgaacag gtttggcggt ttgcacttct atttgctgcc
     1381 tgtcgcgtgt aatcggctgg gcagtaaatg agtataaccc atagctctgc agttgaaaat
     1441 tctgcgtatt gtcgggtaag aagcggatct ggccttcaac atccattgtc agtctgccaa
     1501 ttccggataa tttaaccggt tttgactctg aaagagctgt tttgatctgt gatacgaaat
     1561 caacaatcag tttatcggca tcgcgatgtg aaattccttc ctgacgggca atagtatcgg
     1621 ctagcagtcc gtcgttggtt gagaggctca cattgaaagc aacagatttg ccgggactat
     1681 tgaaaataaa tgaggtagga tgaactttcg caggttggta gttggtaaca aagccgccaa
     1741 gaccgggcac aatcacgcaa ttgtgttcgt acagtagttt gacgatatgt tgtgttacct
     1801 gatacactat ccccgtgttt caggcaaatt tcggctgcta acacacaatt aacaccctaa
     1861 agtaatccac cgactgttca aaacgcaaag ctcaaccctg ccatggcctg aatgccatag
     1921 gaaggatagt tgtaatagcg ctgatacttg taattggcga gattgttcag gttgacgaat
     1981 atgttgaaat acttagaata gacgtaagta gctgaaatat tcatatcaac acagccctta
     2041 acttcttcgg ttatgtcgcc gggaagcaaa gccagcgttt tattcacccc aaatacatcg
     2101 gcagtcatag acatgcgttt gttaaagcgg tacattcccg aaagtgtcca ttccaacgtt
     2161 gggcgatgcc aaggctgatc gaggtctgca acttcagaaa acttaaaggc attcactgaa
     2221 cctgtcacac ggaaacgatc tgaatcaaag tagctcattt cgagggcacc accccaaatt
     2281 gtcgcgtcgc tgtatttcac gaggaaacgc ttcatatctg tagtatcatt tacatagaat
     2341 gggaggtctt gggcagattt ctgatatcct ttaaccgaga aactgaaatt tccgctggtt
     2401 gaacccctca gcccggcaaa tttttcatgg atttcagaga ttttgaaaat gagtgaatcc
     2461 gcaacaaatg gattttctgt aacaatatta gcaaagctgt tcacctgagt ccttccctgc
     2521 cagcctgcta cgaaaacgaa cttatcgcca attaagtcat aactaaatac cgcatccggt
     2581 aaaatgtagg cattttgatc tttatccatt cgggaatcaa gtccaacggt aagtgaatat
     2641 tgatcggtaa tcattttgta ataaggtttg atacctacca gtgaattata ataatcacca
     2701 aaaccggtgt attgataata ttcatacgat gcggaagcac cggcaatact attattctcc
     2761 aatttctttt caccccatgc attcaggaag gggttaattt ctttgtattt attgatatca
     2821 ctgatgcctt taagtccggc tttgagtccg aaatccatat tcaggggatt atcgcctgta
     2881 ttagcgaaac ctaaatcaaa accataattg gtaaatcttt gtttcaaatc agcatcggta
     2941 taggtagtgt ccacctcatg atcgaaacca taataattca aagaatttgt tgagaaatag
     3001 gcattgacag gcagcacata tttatcgtcg aagaagaatt ttgctgtacc tcctaccctg
     3061 gtatctgaat aattttgaaa atcgatctta ctattcgacg aaatatattt tccatacaac
     3121 ccgtaattat acttcttgct gcggtttgaa tttaaatagg cttcggccaa aggtgtaagt
     3181 tgcgtaccga atccggcttt aacaaataca ttgtccaatt cttccggttt cacatcgggc
     3241 atacggatag gtttaacctt caccggctga taaggtatct ggaaaaattc aatcgggata
     3301 ttgtacgttt gcggatccgg tttaatatcc tgcttaggca agcttgcttc aaatgtttct
     3361 ttgacagcat cagcaagtac aggattgtaa ccactcacta catctacatc ttcatctcca
     3421 acgatctgag ctattgcctg cgagctaatt agcccggtaa aggccagata tattgcgaat
     3481 ttcttcatag tctttgtcat gttaatcatc aattattgtc gaaattgatt tctgattcat
     3541 cattggtatc tgaggccgga cttttacgtc gacctgattt ttccatttct tcgatttgct
     3601 cgagtttttg tttagcaagc tcaaccaatt ccgggtcacc tttatagcca ctgataatac
     3661 tattcaaagt agccttagcc tgcgcataat cgccttttgc agctgcgatg tctgctaata
     3721 aaatatagct cctgattatc cactcatcgt acgaaggcat attgttgatg atctcattac
     3781 atttagtttt agattcctca agttttcctt tttggaataa gatatcagcc atatgaaagc
     3841 gtgattcaac accaatttca ttcgtagtca atgcagcagc cttgctgaaa gcatcatacg
     3901 ctttgtcgat attattatta gccagatgcg cttgtcccga atagtagtgc gcatctattc
     3961 gttctttatt tgtaaccaga tcgctggcaa ggatacgatt tgcatttaca atcaccgcat
     4021 caaaatcatt gaggaaataa gcagtctgca ataaccctac catggctaca aaggtgtttt
     4081 ctttgaaatc agccacttca tacaatcgct cataatagtt gtatgctgtt tgataatctt
     4141 cattctgata atattcaatc caccctgctc tcacgagtgc tgattccagg aatttagttt
     4201 tatttccttt aattatagat tcaaaatccg gcaatgcttt agcatattgc tttaattgat
     4261 aatatgattc tccgcgatag aattcagctt cagatttata tccaccttta gggaattcgc
     4321 gcaaataatc agagaaggct atagccgctc ccgaaaactc gttcaggtcg tatttatttt
     4381 tagcgtagtt atatttttct tcatcaatct tagtcacttc aaccacgcca tcggcagcgc
     4441 cagtattacc ggtttctttt acgatatcgg caagtccttc tttagcttgt tgtccttctt
     4501 cagagtttgg agaactttcg taggcgaggc gataatattt ctcagagttt ttgtaatctt
     4561 tctcattgaa ataaatcaaa ccaagtttga gataggcctt aatggtgtaa ctgctattag
     4621 gtttttcact tactagttcc ttgaacgcag caatagactc tgtgttcttt ttagtgatga
     4681 agtatgtatt ggcaatttca tacatagcat catccacata caatgaattc gggtatttgt
     4741 tggttaatga ttgcagtgtt gcaattttac cattgctgtt attttgtaac ccctgcaaca
     4801 tcgctttctg atacatcgca taatcaaccc ctttggcatt gttgtcgatt attttattgt
     4861 agtttgtttc tgccttctta taatctttcg tcataaaata gcagtcacca agacgaagag
     4921 atgcatcgat gtaaacatta ttttgaacca tatcggtttt actggtcttc aaagcagtag
     4981 ttactttgct gaaatagttc accgcatttg aataattgct ttgtttgaag taagcataac
     5041 cgatagtata atcgctataa ggaacacgtg tttctgacgg aaggtctttg gcaactttag
     5101 cgagatcttg aaatttaacg tgatttgtta tggtgagtgc atatttattc tcagcaaacc
     5161 tgatctcgcc aatccagaaa taacatgctg cttgcaaatt cacatctatt ggattttcca
     5221 gtgattgcat gaacatttta tcagcttcat caaagttttt ttgattaaac aattgcacac
     5281 cggaataata cgacaccttt tgataggctt ccttcaattt tggtgttttt tgagggatgt
     5341 tttcaattac atccatcgca gccaggtaat cgtttgtacc gagtaatgca ttcgccaata
     5401 attcttgtgc ttcggttttg ttgcttcctt taggatatgc cttcaaataa tcttgcattg
     5461 cagttacagc agcggtatga tatccggatt catatgaaag cttgccataa ttaaataatg
     5521 cttgctcgtg aatggttttg tcttcaccaa ttcgcgaagc ttccataaat gcattacggg
     5581 cttcttcttt tttatcgaga gcgatataac aattgccata taaatattga atatgctgat
     5641 acaaggtatc attatcatcg ctagtctcct ttaatgttgt cagagcagct ttactgtcac
     5701 ctgtagaata ctgtgcaaat gccagttgat aataatcttc atcgcgaata ttctttgcct
     5761 tttcaatgta atagttcaga tatggtaagg ctttgcggta attattccga tagaagtatg
     5821 cctgacctat taaactgtta atttcggcaa aatacacgag tttggtatca gccatcagcg
     5881 gttctgcata cgaaatcacc ttttcatatt ttccttgttg aaaataaata ttggtgatgt
     5941 aatatggcac aactgcatta tatggcggca catcttttac gatgttaaaa caggttaacg
     6001 cattttcata gttgccatct tcctgctgaa taaatccgta ataataatta gcagcgttat
     6061 aatattgatt ttgcggtttg tcttttaatg atgcgaacat cactttcgcc tcggtaaaac
     6121 gttttttgtt aaacaggctg tagccgtatt gaaatttgta ggtgtcgcgt tcttcaagcg
     6181 aaagatcttt aacatccacc ttttcaaacc aggtagcggc attggtatac tcttttttat
     6241 tgtagtataa tttaccgagt tggttgtagg ccgaaccaag cattacgttg ccgggattgt
     6301 tatgtacgaa atcgagcatc attttttcag catcggtatt gtttaactcg atagcgcata
     6361 tagcagcata atagcggcta ttcgacacgt aggtgaagat atcgccgtca gttccctcgg
     6421 gcgtaaccga gcctacttgt tcaaagtatt cttgtgcggt ggcatacatc ttattctcat
     6481 agagctccat cgccttttta taattggcat acagatcggt ataaatcagc gattgttgtg
     6541 ccttcagtgc tcctgtagtg ccgcataaca cgatgcacat acaagctatt attagtttgt
     6601 ttctgatcat aagttgttcc ccaaaagggt ataattatgg ctaatctaaa atcgcgccga
     6661 aattacggct attaagagta gaagcgttca atccattgat aaatgaataa acaggtgttt
     6721 gttgataatg aacgccttag ggagaaaaaa tagtatgtat aggctataac aaacagcaga
     6781 attgctattc tacaatttta tctatccagg tcatatcgcc ataatagtac caccggttgt
     6841 taaatttcac agcggtgaat tctaccttac ggggagaacc atcagcgtca aacttagtag
     6901 taacgcgtgc ctttggcaat ttgccgagct tagattcagt gtaattacct cgggtgaaag
     6961 ttgcggatga ccagttactc accttattcc tgactgtatc caaacacgct gaggattgct
     7021 tttcgaatcc gttgtatccg gcttcaaggt tgactaagat atgtccattt tcctcttcta
     7081 tctttctgaa agttccttta tcaggtaata gcaatgctat tttttccggc gaattagatt
     7141 tcatagcgtc aaatagcttt ttcgacattg tttcggggtc caatgtgccg cgttccggtt
     7201 cgttgcagga aacgattgcc agtaagagga tcgggaaaat aagtcttaat ctcatgtgcg
     7261 tggttgcggg ttaaaagcgg atgtaaaatt tatgtatttt ctttacgtgg acaaatactt
     7321 ggacttacgg taagttaaaa aagattaaac gagtaaggaa tggttcaaat ttatacagat
     7381 ggttctgcaa ggggaaatcc gggtccgggt ggattcggga ctattttgcg gtacgggaag
     7441 catgaaaagg agttgtcggg aggttatcgc agaactacta acaaccgtat ggagctgatg
     7501 gctgtcattg caggattaga ggcgctgaca aaggagggac ttgtcatcac catttacact
     7561 gattccaaat acgtgtgcga agccattgaa aagggatgga tttatggttg gatgaagaaa
     7621 ggttttgccg gaaaaaagaa tgccgatctg tggactcgac tgatgcgcgc ctatgcacgt
     7681 cataaagtga agttgatctg gatcaagggt catgccggtc acccggaaaa tgagcgctgc
     7741 gacgtgctgg caactacggc tgccgatggg cacaagctgg ctattgatga ggtgtacgag
     7801 atggaaaacc aaacgaaact aggatttgat tagtctaaac tgatggttta acagtctaaa
     7861 ctaacaccac tctagtttag actttagctt cagtaaacct atcgaggaac tctacgattt
     7921 tctccaccat ggccgaagaa ccaatgtaga atggtgtttt ctggtgcaac tccttcggtt
     7981 cgatgtcgag gatacgcttt cttccgtttg tagctactcc cccggcctgt tcggcgataa
     8041 atgcgagcgg attgcattcg tagagcaaac gcagtttacc ttttggcgat ttagccgttt
     8101 cgggatagag gtatacccca cccttcagca tattgcggtg gaaatcggca accagagacc
     8161 cgatatagcg actggtatac gggcgttgcg atttaaggtc ttcctcctga gtatatttga
     8221 tatagcgttt cacaccttcg gggaagtgaa catagtttcc ttcgttgatg gaatagatga
     8281 taccgcggtt ggggatacga atatcggggt gggaaaggca gaattcacct attgatgggt
     8341 cgagggtaaa gccattcacg ccaaaaccgg tggtgtacac catcatagtt gatgacccat
     8401 agatgatata gccggctgca acctgttctg tgcctttttg caggaaatcc tccaaagtag
     8461 cgccaccgct gagactcagc ctgcgataga tgctgaaaat ggtgccaatg gacacattca
     8521 cgtcaatatt tcctgagcca tccaatggat cgatgagcac gacgtactta cctgattttg
     8581 aaattgcacc atcgaaactg atgaagttgt cttcctcctc gcttgcaaat ccacatactt
     8641 cacctccggc catcagggcg ttcatgaatt ggttgttggc atatacatcc agcttttgct
     8701 gggtttcacc actcatattt tcgctgcctt ctgcgccgag tatatcaacc aaccccgcct
     8761 tattcacctc acggttcacg atcttacctg ctatgcttat atcggaaagg agtcgggtga
     8821 gttctccggt ggcaaaaggg aagtcctgtt ggcgttctac aatgaattct atgagcgttt
     8881 ttaccttcat aaagcgtggt tagggctgca aagttaacac aaaatgtccc tgccccgatg
     8941 attttactca cctaaacgta gatttgtgac atggaatatc acattcgacc tgccgatggt
     9001 gctgacatcg ccatgataca caggttagtt tcacaactcg ctgaatatga acgtgctgcg
     9061 gatcaggtga tcactactac tgcagattat cagcgcgatt tcgaggccgg tcgttttcag
     9121 gcattagtgg cggtggacct gcaatccggt gaaattgtgg ggatggcatt gtattacttt
     9181 gcatactcga catggaaagg ccgatatctg tggcttgaag attttatagt cactgagcaa
     9241 tgcagaggta agggagttgg tcgattgctt tttgatgctg tgggagctat tgcagcgaag
     9301 gaaaacacat ttatgaagtg gcaagtgcta gattggaaca cgccggcgat taatttttac
     9361 aataaatatc cgatggaagt gcaggatgaa tggttaacct gcagaatgcc ggtgcgttca
     9421 tgattaatac cggaaacggt ggcggattgt ttactccgtt tcatacaaca catcccgaag
     9481 tatggggatg atggtgagaa aatagagcac atgaatgatg attattctga acatccacaa
     9541 aatgaatact tcaccgagat cagataagtc caaatcggcg ttatcggtat acattccggc
     9601 aatccaaatt acgggcattg cgagtaaaat catccatgcg aagcggaatt ctttgtttat
     9661 ggcgtaaatg atgatgccca ttatttggac aattgatccg aaaatcatga tttcccacca
     9721 atcgggatgg atgtttggcc ttgtaaagaa aacaatgaac catagatgac acatcatcca
     9781 gcaagccaat tgcacgatac tgtggtatcg catgaatgct gttggcctgg gttgattttt
     9841 ggaaagttgc ttgcggaggt tcatggggaa attgggtggt ggtggggata aaattctgaa
     9901 aaaaagttgg caatcagtca atgctccaaa cctggcgcgt tttaaaccta aaaggtctca
     9961 aacctaacag gtcttagacc tgttaggtct gattttgaag aactccctcc taccatgctt
    10021 attacccgaa taatcgttta taagcgagta ttcgcgataa tttacaggca agaaatacat
    10081 gagttcagaa atttcgaaat ttgccgtggc aaggtcactt gattcgcaag gtcaaggcgg
    10141 cttcactttt attggtcctg atataatccc atgattgttt atcattcaga ttcgtaccta
    10201 ataaaactga atccttccaa attcttacat ggtatgagag gtcaactgaa tcctggtttt
    10261 caaatcgatc cacatagcct aaaaaacact gctgaaataa aatctgctcg gtatgctcag
    10321 gaatgcgtat ttgtgtaact ttattactgt catattcaaa atatgtaacg tcaactaccc
    10381 cattgctttc attggcaatt ttaaaagtga tacaagaacc ggggtcgcaa gcaatcagaa
    10441 ataaaattac cggaagaaac acgatcgctg cccgactagc attatttgaa aaaaagtgcc
    10501 gcatattctt tcaagtatca attaaatcat tccttcctca atattttctc tatggccctt
    10561 cctttggcta actcatcaac caatttatcg agataccgaa ccttcctggt taatgggtcc
    10621 tgtatttcct caatgcgata tccgcaaatc atacctgtaa tgagctttgc attaggattg
    10681 agtttcgcct tatcaaaaaa atcagcaaaa ttgacacgct tttcaattag cttttgtaaa
    10741 gttacaaggt tataccccgt aagccattca atcacctgat gcaattcatc cttagtcctc
    10801 cccttctttt cgatcttagc gagatataat ggatatactg aagcgaaggt catcgccgcg
    10861 atacgtgctt catgtgattt agttgttgcc atactcgggg gtttatttat taaagataat
    10921 gcttagcaat aaatctacaa tgatgtaaat tatgtccaac agtcataaag ctatgttcat
    10981 attaaaacta caaataggct taatactttg aaacagtcaa catactatta atcaaataaa
    11041 caccagcatt gccttacgtg catcttgata tctattgagg gttttctgca aagtgcagaa
    11101 cattttatac ctcctaggct cactaaaatc acctttcatg atgtgattgt cgtagtcata
    11161 ccctgaagta gaaaaaatgc tccgcttcgc tgcacatttt gtcacttcag ggcaggc
//
LOCUS       DAOQOU010000102        11216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_2988_length_11216_cov_6.441000, whole genome shotgun sequence.
ACCESSION   DAOQOU010000102 DAOQOU010000000
VERSION     DAOQOU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_11216_cov_6.441000"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..786)
                     /locus_tag="PLM90_09465"
     CDS             complement(199..786)
                     /locus_tag="PLM90_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89643.1"
                     /translation="MHIFDPAEAALFYNPDVFAVKRSATQKLYAAFEDLRLRLKDTGE
                     QLQFPFPNEVTSISAKISQGENYGGCPWVMLDYHRYFKERDMFAFRVMAWFGHYFSAH
                     LVLGGRFINVYNRNVKDNLEGSDVFFTLHDTPWEHAVGTPISLPLKGLTPAQISTQAD
                     EHQFVKYSICIPSADLSKVIDEVVAFYRCSFNTVT"
     gene            789..2216
                     /locus_tag="PLM90_09470"
     CDS             789..2216
                     /locus_tag="PLM90_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMZ89644.1"
                     /translation="MTKIDKYIIKSFLGPFIISFIIALIIFLMIFLWKYIEDLVGKGL
                     EWYVIMKLLFYASANMVPLALPIAVLLACIMTYGNLGERLELAALKSAGLSMFTFMRS
                     TLLIGVFIAIGAYFFNNYVLPSAYLKFVVSLVDITKQKPALNIKPNEFYNEIDGYSIL
                     IGGKSDDNVTIHEVTIYEELDHSNDNVLIAESGKMQTTSDNNNLVFSLKNGKQYEQLA
                     PTGKGKRHVQHVRMHFAEWNKSLDLSGFQMTHTSEELYQNHFKMLNTHQLSHAIDSLK
                     TNNNNQAQALAPTLRVSYLQGRAPGVTFNDTAYAHQASSFIELIPLAQRLPVIKKSLQ
                     SVRTQTGYTNQVLMRMKNTRTKINSHLIELNRKYTLPLSCLLFVFIGAPLGAIIRKGG
                     FGLPVLLSIIIFIFYYVLNITGEKMAEQSALSPFVGMWLSSFILVPICVFLTIKAKND
                     APIVDGEWYYNQLVKLKGVFSKIKS"
     gene            2213..2863
                     /locus_tag="PLM90_09475"
     CDS             2213..2863
                     /locus_tag="PLM90_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ89645.1"
                     /translation="MIGIIRHNKEFYLLWAITMIIVGAILPFTEKSDFSLWLNFRHTQ
                     AADFFFRYATWLGDGFVIGVICLILFILKLRWGIIASVGSFFSALFINLIKKEYNEPR
                     PSVVLKNMDLHYVKGLDLYSNFSFPSGHTAAAFTLCVLLCYFAQDKRYTRLFFALAVI
                     VAISRVYLLQHFLVDVYFGALLGTIFGTILLWPFIRIPLFSRPWSEIGILSGNRKV"
     gene            2958..3218
                     /locus_tag="PLM90_09480"
     CDS             2958..3218
                     /locus_tag="PLM90_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89646.1"
                     /translation="MRYKLTILNACSAAFMIGLIIYAMTNYSDLKNEDGFGMAAVYGL
                     AGLSLMGGMMDFLLQALVKHEGTMNILGSFVAITIGMVVALS"
     gene            3509..6310
                     /locus_tag="PLM90_09485"
     CDS             3509..6310
                     /locus_tag="PLM90_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89647.1"
                     /translation="MKTNKRTSIKPIAIYILLLIFIGSNLFSAIGKNTESYRKNELTF
                     QKSGSTLIVFPDSVPVELDTSTAFEIGDTIDINLAGLDTLFILYEDTNSIGGDTVSEG
                     NEFRLVDATKDITITPGFLPKEVEHGLFGYHIEGIFTSERLPQDTANVNFPQSWRWMA
                     DLKPQVLRFPGGASSRWMHLLPYQDAEAPFDTLDPIKGYGYDITEIIRYYDGTDGDID
                     AEDSVYVNSIIYDLADASCDSCDNWMYAFKYADDFEKLYDKWINQENNIPTNQQQRYI
                     DQFIALVDSIQQWESYTVDVIVDLNVISESATDCRHIIDYLRNPAPKAEGGNGVTAVN
                     VVGVEMGNECNLNWGTDIMGWKGFNDYWYLINGKGQADIPDGYFSDDYNTWLDSLSAY
                     VFSPEYYEDHDFISAFKSDPDLEIKVGIPAANLKNNDSTKFAFRMMEDLSTNWNDTLV
                     THFQDSIVVGDSTRYLFDAVVLHTYYDAQTNWDSLATSNLCDSLYPNSGIAGGCVTED
                     CDDWTNDQWQFDITDERLSNAYKAVIGLGSNGFGNFKQFIRTRYAESYDQQRLDLMFY
                     GNEAWKKDLWTTEWNLKDKNTDYSANDPEQYILSSFCNSFEHGWLIQEWFLKDIKQNY
                     AKNYRKGFHTYSTFHAFGGGSYYTLLLQSDRADREKHLDELGNPDTLITPPADQNLFL
                     KRTMYHTFDMLSEIKNHNLKYLPSNFTAYVHNPNIQPTVFIDKSSKKLYIYYSNMKTE
                     TQSYVLEKGNILMLYPGSTALAYGEALIYNIAPTQLYSNSGNSFLYKMNICYNTTDQQ
                     HPFEIQYINGPVINEPECSGLPGGAICLTVPANSYGYIEVPFYITPREGLTLSDDQIE
                     VYPNPTANAFRITCDIPEELLNTFYVSVFDIHGSLLLNTVISQNQDIDISNLPSSIYF
                     IVISNEEKSFSVSKKIIKI"
     gene            6310..7203
                     /locus_tag="PLM90_09490"
     CDS             6310..7203
                     /locus_tag="PLM90_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89648.1"
                     /translation="MRAKKQFTLQQYSCLAGALLLIQNNSFSQAIYTDIEPDVELQFQ
                     GDFVYIDMDSDSNNDFFLLKRSSYFTNTYYEWYRFNHILKCGPNFNPENSIAGIFATN
                     GAGGGTTYFPYAINAGEIINSDLSFQYGHYQLMGIGFFDVDETPWVWHYFIGSWTPSI
                     DSMFLGVRFLGGDDCKHYGWIRCSTADSAKTLIVHDFAYESKCETPIIAGDTIGDTTT
                     VAVSNLLELNAEIFVYNKQVFVSCKVSHQLEIFDSSGKKILDSNRQQGAYNIDMQPFP
                     KGIYIVKLTSGSQMISKTISM"
     gene            7608..7970
                     /locus_tag="PLM90_09495"
     CDS             7608..7970
                     /locus_tag="PLM90_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ89649.1"
                     /translation="MQGLRTCIYKVTDLEAGKAWYSKAFNQTPYFDHPFYVGFDIGGF
                     ELGLLPEEEEKHVYGNNMLVYWGVDDIQSSYDHLVSVGATSHEAPHAVGDDIMVATVF
                     DPWGNIVGIIYNPGFKMA"
     gene            8078..8524
                     /locus_tag="PLM90_09500"
     CDS             8078..8524
                     /locus_tag="PLM90_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89650.1"
                     /translation="MHTLSAQSEDHAKYISLELAGSGGLGSINYEQDFKDFGKWDLTW
                     RAGFSLAPIDKNNGVGLVFPLMINARFGEGTSKLDMGLGQGVTITTKGAFFMLTTAAV
                     GYRYQKETSPWYFKASYTPLISYLVDFQWQHWGGLSIGYTINTSVK"
     gene            8521..9285
                     /locus_tag="PLM90_09505"
     CDS             8521..9285
                     /locus_tag="PLM90_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89651.1"
                     /translation="MKRTVIAALLIVTIVGCFDHAYFEGPDQFDEDFEAYHSVDDMFV
                     DDDVLWSFTQLTMPENTLIIDSTFSHGGLKSIKCFAQAGAEGTVSKASFSKQNMAFWE
                     GETMRASGWYYIPGDNELDWLFIMDLEEQTAIGAGPGIRLAIVDNCLMVEHKYLEDDI
                     YQPTTSQIPFPRNEWVYLNWEVYLSTKDDGTIKLYQNGELILSNNHTVTLPQDILYFQ
                     QGTKGMYSSIEFGATANPTGADAIVWVDDIFVEVVK"
     gene            9370..9957
                     /locus_tag="PLM90_09510"
     CDS             9370..9957
                     /locus_tag="PLM90_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89652.1"
                     /translation="MKRPELIDLTLKITGIIMVIQLVNSVIQLIFTIPQITFIWQGPD
                     DGQFSYVIALFGIYVLGPLIYAVVIYQLTFKSDKWARRILRPHQMEEEINLKIEPQVM
                     LNVSILIASIFLLFWNIPTLLMAFGQFIASNTPRHMDYMPVDFDKNGLIEPILNIAVA
                     LILFTWSSPISHYLANRIVKATPSEEQEQPKIDDI"
     gene            9954..10427
                     /locus_tag="PLM90_09515"
     CDS             9954..10427
                     /locus_tag="PLM90_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ89653.1"
                     /translation="MTSQLSQQIAKQFRDAHDGDNWTAVNLKTLLSDVTRDEAVQQIE
                     GFNSIATLVNHTGYYLDIVIPVLRGAPLVGKDSDSFILPDLSDQEKWQALVDKVFADV
                     EVFAALIESFPDEKWWEPFNDGKYGNYYRNITGIIEHLHYHMGQIAFLKKMLRKS"
     gene            complement(10526..>11216)
                     /locus_tag="PLM90_09520"
     CDS             complement(10526..>11216)
                     /locus_tag="PLM90_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89654.1"
                     /translation="YDDNGTATGIYNSWDIKSGGSYVYILYKQDARFSSGSWYLYIDY
                     DWTNSGTLTAYETLPLEPARDKNWLVYDFKFTDAGNYRAYIMHDGVELASVNFNIGWQ
                     EGEAPTTSGSSTIDTYYYEDSELKFCGSVDAEGNPMGIATEFYMGSNGKASFKAYLDN
                     DFKAIQTSLIYVDVYKEGVTDPVDAFSITVQPDWDYIHFQLDLTSAGLYHVDLYNADD
                     IFINTADVVIR"
BASE COUNT         3465 a         2219 c         2182 g         3350 t
ORIGIN      
        1 agctgcagaa gccctgagct tttggctcca ccttttggct tcaaaaggtg gaagatagtt
       61 gtcatgcgga gcaaagttgt ggtttaaagt attaattcga tgaaacgaaa acttcgcttc
      121 gcaaaaaact ctccgctacc ctttttcttg acaaaaaggt agcaccaaaa gtcaagggct
      181 gcttgcggcg aaaaaccatt atgtaactgt gttaaaactg caccgataaa aggcaactac
      241 ctcatcaatg actttggata aatcagctga tggaatgcaa atcgaatatt ttacaaactg
      301 atgttcatct gcttgcgttg aaatttgtgc aggagttaaa cccttcaaag gcaaactaat
      361 cggcgtcccg acagcatgtt cccaaggggt atcgtgcagc gtaaaaaaaa catcactgcc
      421 ttccaaattg tccttaacat ttctgttgta aacgttgata aacctccctc ccaacacaag
      481 atgcgcactg aaataatgtc caaaccacgc cattaccctg aaggcaaaca tgtccctctc
      541 cttgaaatat cggtggtaat ccagcatcac ccacggacaa ccaccataat tttccccctg
      601 tgagatcttt gcagatatgg aggtaacctc atttgggaac ggaaattgta attgttcccc
      661 cgtatctttg agacgaaggc gcaagtcttc aaaggctgca tacagtttct gtgtcgcgct
      721 gcgtttaaca gcaaaaacat cgggattgta aaacaatgca gcctcggcag gatcaaagat
      781 gtgcatagat gacgaagatc gacaaatata tcattaaatc attcctgggt cccttcatta
      841 tcagttttat catcgcactg atcatattcc tcatgatatt cctttggaaa tatattgagg
      901 atctggtagg caaagggctt gaatggtatg taatcatgaa attgctgttc tatgcatccg
      961 ccaatatggt gccgcttgca ttgcctattg ccgtgctgct ggcatgtatc atgacttatg
     1021 gcaaccttgg agaacgactt gaacttgccg cgttgaaaag tgcaggattg tcgatgttca
     1081 ccttcatgcg cagcacatta ctgataggtg tgtttattgc gattggcgct tattttttca
     1141 ataactatgt acttcccagt gcttacctga aattcgttgt ctcccttgtc gacatcacca
     1201 aacaaaaacc ggcactcaac atcaaaccaa atgaattcta taatgaaatt gatggttata
     1261 gtattttaat cggcggaaaa agtgatgata atgttactat tcacgaagta actatttatg
     1321 aagaattgga tcactccaac gataatgtgc tcattgccga aagcggtaaa atgcaaacca
     1381 ccagcgataa taacaattta gttttctcac tgaaaaatgg caagcaatac gaacaattag
     1441 cgcctaccgg aaaaggtaaa cgccatgttc aacacgtgcg catgcatttt gctgagtgga
     1501 acaaatcgct cgatctcagt ggctttcaga tgacacatac cagcgaagag ctctatcaga
     1561 accatttcaa gatgctgaac acgcatcaat tgtcgcatgc cattgactca ctgaaaacca
     1621 ataacaataa tcaggcgcaa gcacttgctc ccacccttcg ggtttcgtat ttacagggac
     1681 gagcacctgg tgtaacattc aatgatactg cttacgctca tcaggcatca tcatttattg
     1741 aacttattcc attggcgcaa cgcttacctg tcattaaaaa aagtttgcaa agtgtccgta
     1801 cacaaacagg ttataccaat caggttttaa tgcgcatgaa aaacacacgc acgaagatta
     1861 actcgcatct catcgaactt aatagaaaat ataccttgcc attatcctgt ttattgtttg
     1921 tattcattgg tgcgccatta ggtgccatca tacgcaaagg cggatttgga ttgccggtat
     1981 tattaagtat catcatattt atcttttatt acgtcctgaa tattaccggc gaaaaaatgg
     2041 ctgagcaatc tgccttatcg ccattcgttg gcatgtggtt atcctcattt attttagtcc
     2101 cgatatgtgt attcctcacc atcaaagcca aaaatgatgc tcctattgtt gatggtgaat
     2161 ggtattacaa tcaattggtt aaattaaaag gagtgttttc gaagattaaa tcatgatagg
     2221 tatcatccgc cataacaaag aattttacct gctatgggca attactatga tcatagtggg
     2281 agcgatacta cctttcaccg aaaaatccga tttctcttta tggctgaatt ttcgtcacac
     2341 gcaagcagct gacttttttt tcagatatgc cacatggctt ggtgatggtt tcgtaattgg
     2401 tgtaatttgt ttaattctat tcatattaaa attgcgctgg ggaataatag cttccgttgg
     2461 cagttttttc agcgcattgt ttatcaacct catcaaaaaa gaatataacg aaccaaggcc
     2521 gagcgttgtg ctgaaaaata tggatctgca ttatgtaaaa ggactagatc tttacagcaa
     2581 tttcagcttc ccgtcagggc atactgccgc cgcctttaca ttatgtgttt tactttgcta
     2641 ctttgcgcag gataaacgct acacacgctt gttttttgcg ctggctgtaa tagttgcaat
     2701 aagcagagta tacttgttgc agcactttct tgttgatgta tatttcggcg cactgttagg
     2761 caccatcttc ggcacaatcc tgctctggcc ttttataaga attccgcttt tctctcggcc
     2821 atggtcagaa ataggcatcc tatccgggaa ccggaaagtg tgatcccggc cgaaaattat
     2881 caattcctta ctctatgtgt ctatgtttag gcaaatatgt taaattagct atgtgctaat
     2941 ccaaacaatc tattgctatg cgctataagc tcaccattct caatgcctgc tctgcagctt
     3001 ttatgatcgg attgattatt tacgccatga ctaattacag cgatttaaaa aatgaagacg
     3061 gctttggtat ggcggcagtt tatggactgg cagggctaag tttaatggga ggcatgatgg
     3121 acttcctgtt acaggctctt gttaaacatg aaggcaccat gaatatcctg ggctcatttg
     3181 tggcaattac tatcgggatg gtggtggcat tgagttgaca gatcaaattc ccccactttt
     3241 gtcataaacg acccgacact gaatgtatta aaatatttag ttgcacagct tgttattaat
     3301 ttaaaaccag taatttaata atattgtatt ctgaattact ttttttccaa ataccatttc
     3361 aatatgtaac agcaattact caacaataag aaaatagtat aatgtaattc ctgagtcggc
     3421 tcaatacctc atttatcagt gctaggtaaa aaataccatt tattaattta ttattaaatt
     3481 tattcataat tttattaaaa tacaccttat gaaaacaaat aaacgaactt caataaaacc
     3541 aattgcaatt tacatattgc tattgatttt tattggatca aacttgttta gtgcgattgg
     3601 caagaacaca gaatcctacc gtaaaaatga attaactttt caaaaatcgg gaagtacatt
     3661 aattgtattt ccggattctg ttcctgtcga actcgatacc agcactgctt ttgaaatagg
     3721 cgatactatt gatattaatt tagcaggatt agatacatta ttcatacttt atgaagacac
     3781 gaattctata ggtggcgata ccgtttctga aggaaacgaa ttcagattag ttgatgcgac
     3841 aaaggatatc acaataacac ccggtttttt accaaaggag gtagaacatg gacttttcgg
     3901 ctaccatatt gagggaattt tcacatctga acggctgccg caggataccg ctaatgtaaa
     3961 ttttccacaa tcgtggagat ggatggcaga cctaaaacca caagtcttgc ggtttccggg
     4021 aggagcaagt agccgctgga tgcatttatt accctatcaa gatgcagaag ccccatttga
     4081 tacgcttgat ccgattaaag gttacggata tgatatcaca gaaatcatta gatattacga
     4141 tggaacagat ggcgatattg atgctgaaga ctcagtgtat gtgaattcta ttatttatga
     4201 tttggcagat gcttcatgtg atagttgtga taattggatg tacgcattta aatacgctga
     4261 tgatttcgaa aagttgtatg ataaatggat aaatcaagaa aataatatac ctacaaatca
     4321 gcaacagcgg tatattgacc agtttattgc ccttgtcgac tcaattcaac aatgggagag
     4381 ttataccgtt gacgtaatag tcgatctaaa tgtgattagc gaatctgcaa cagattgcag
     4441 gcatattatt gattaccttc gaaaccctgc accaaaagca gaaggaggaa acggtgtaac
     4501 agctgtaaat gtggtcggcg tcgaaatggg aaatgaatgc aatttgaact ggggtacgga
     4561 cattatgggc tggaagggtt ttaatgatta ttggtactta attaacggaa aaggtcaagc
     4621 tgacatccct gatggttact tctcagacga ttacaatact tggctcgatt ccctttccgc
     4681 atatgtattt agccctgaat attatgaaga tcacgatttc atttcagcat tcaaatctga
     4741 cccggattta gaaataaagg ttggaatccc ggcggccaat ctcaaaaata atgactcaac
     4801 caaatttgct ttccgcatga tggaagattt gtcaacaaat tggaacgata ccttggtgac
     4861 gcattttcaa gacagtatag ttgttggtga ttcaacccgt tatttatttg acgcggttgt
     4921 cctacacacc tactacgatg ctcaaaccaa ttgggattca ttggcaacta gtaacttatg
     4981 tgattctcta tatccaaatt cgggcatagc cggtggatgt gtcacagaag attgtgacga
     5041 ttggaccaat gaccagtggc aattcgatat aactgacgaa cgtctaagca atgcatataa
     5101 agctgtgata ggattgggtt ccaatggatt cgggaacttt aaacaattta ttcgaacaag
     5161 gtacgctgaa tcttatgatc aacagaggct cgacttgatg ttttatggga atgaggcttg
     5221 gaaaaaagat ttgtggacaa cagagtggaa ccttaaagat aaaaatactg attactcagc
     5281 gaatgatccc gaacaataca ttctaagttc tttctgtaat tcatttgaac atggatggct
     5341 tatacaagaa tggtttttaa aagatataaa acagaattat gccaaaaatt atagaaaggg
     5401 ttttcacact tattccacct tccatgcctt tggtggtggc tcatattata ccttgctatt
     5461 acaatcagat agagcagatc gagaaaaaca tcttgacgaa cttggtaatc cggacacctt
     5521 aataacacca cctgcagatc agaatttgtt tttgaaacgt actatgtacc atacatttga
     5581 tatgcttagc gaaataaaga atcacaacct taagtacctg ccctctaatt ttaccgctta
     5641 tgtgcataat cccaatatac aaccaacagt ttttattgac aaatcaagta aaaaactgta
     5701 tatttactat tccaatatga aaaccgaaac acaaagctat gtattggaaa aaggaaatat
     5761 cttgatgctc tatcctggtt ctaccgcttt ggcctatggt gaggcattga tttataatat
     5821 cgctcctaca caactctatt ccaatagtgg gaatagtttt ttatataaaa tgaatatctg
     5881 ctataacact actgatcaac agcatccgtt cgagatacaa tacatcaatg gccctgttat
     5941 caatgaacct gaatgctccg gcttaccggg aggtgccatt tgccttacag tccctgcaaa
     6001 ttcctatggt tatattgaag tgccatttta tatcactcca agagaagggc tgacgctttc
     6061 ggacgatcaa attgaggtgt atcccaatcc aactgcgaat gcctttcgca ttacatgtga
     6121 tatacccgaa gaattactca acacattcta tgtatcggtt tttgatattc acggtagcct
     6181 actattaaat acagtaatct cccaaaatca agatattgat ataagcaacc ttccttcaag
     6241 tatctatttt attgttatca gcaatgaaga aaaaagcttt tcagtatcaa aaaaaatcat
     6301 caaaatttaa tgagagctaa aaaacaattt acattacaac aatactcctg tcttgcagga
     6361 gcattgcttc tgatacaaaa taattcattt tctcaagcta tatatactga tattgagcca
     6421 gacgtggaat tacaatttca gggcgatttc gtttacattg atatggatag tgacagcaat
     6481 aatgattttt tcttgttaaa aaggagtagt tatttcacaa atacttacta tgaatggtac
     6541 aggtttaacc atattttaaa atgcggtcca aattttaacc ctgaaaatag catagccgga
     6601 atttttgcca ccaatggtgc aggcggtgga actacttatt tcccctacgc tattaacgcc
     6661 ggagaaataa tcaatagcga tttgagtttt cagtatggac attatcaatt gatggggatt
     6721 gggttttttg acgtagacga aacaccttgg gtatggcatt atttcattgg ctcatggact
     6781 ccgagtatag attccatgtt tctaggcgtc cgttttctcg gaggtgacga ttgcaaacat
     6841 tatggttgga tcaggtgctc aaccgctgat tctgccaaga cattgattgt gcacgatttt
     6901 gcttatgaat caaaatgtga aacgcccatt attgccggcg ataccattgg cgacacaaca
     6961 acagtggctg tgagtaattt attagaatta aacgctgaaa tattcgttta caacaagcag
     7021 gtatttgtaa gctgcaaagt atcccatcaa ttagaaatat tcgacagcag cggcaagaaa
     7081 atacttgata gcaaccggca gcaaggcgca tacaatatcg acatgcaacc tttcccaaaa
     7141 gggatttaca tagtaaaatt gacaagcgga tcgcaaatga tatctaagac gatatctatg
     7201 tgacttggaa gttttttgaa aaggattatt gatatggcca ctttccatca actttgcaat
     7261 atcgcctgtg catttccgga tgtaacgcag gaggcgcatt ttgaaaaaat atcctttcgc
     7321 ataaaaggca aaattttttg cacctacgat ccaaatacga cctcttccac cgttaaactt
     7381 agtcaattgc aacaggaagc atacactaat tcctctcccc acatcgaacc cgtaccaaac
     7441 aaatggggtc accaaggttg gacgcacgcc gatacaacaa aaattgacat cggcatgcta
     7501 aaaaaattac tccacgacgc atatacgctg gttgctgcaa aaccgattag aaaaaaatga
     7561 taggtgtttt cttcaatttc agtatacttg ttgaataatc attcattatg cagggactgc
     7621 gcacatgcat ttacaaagta acagatcttg aagccggaaa agcctggtat tcaaaagcct
     7681 tcaatcaaac cccgtatttc gaccatccat tttatgtcgg atttgatatc ggaggttttg
     7741 aactgggact attgccagaa gaagaagaaa aacatgttta cggaaataac atgttggtat
     7801 attggggtgt agatgacatt caatcatctt atgaccatct tgtatctgtc ggagcaactt
     7861 cacatgaagc accgcatgct gttggcgacg atatcatggt agctactgtt ttcgatccat
     7921 ggggaaatat tgtcggcatt atttacaatc ccgggtttaa aatggcatga catcaattga
     7981 ttcattcgtt cattccgtat actacatttt tatataaatt cgttttttca tgatgcagtt
     8041 gtttcgctgt ttctcgttat gcctcttatt aatgtgtttg catacactca gtgcacagtc
     8101 tgaagaccac gcaaaatata tttctctaga actcgccgga agtggcggac tgggatccat
     8161 taattacgag caggatttta aagatttcgg aaaatgggac ctcacatggc gtgcaggttt
     8221 tagcctggca ccgatagata aaaataatgg cgtcggcctg gtttttcctc tgatgataaa
     8281 cgcacgtttt ggagagggaa cttctaaact ggatatgggt ttgggacaag gtgttaccat
     8341 caccacaaaa ggagcatttt ttatgctgac aacagcagct gtcggatatc gatatcaaaa
     8401 agaaacgagt ccctggtatt ttaaggcatc atacacacca ttaatctctt atttagttga
     8461 tttccagtgg caacattggg gaggattgag tattggctac accataaata cctcggtaaa
     8521 atgaaaagaa ccgtaattgc tgcactgctc attgtcacca ttgtcggatg tttcgatcat
     8581 gcctatttcg aagggcctga tcaattcgat gaagattttg aagcatatca cagcgtcgac
     8641 gatatgtttg ttgatgacga tgtactatgg tcattcacac aactcaccat gccggagaac
     8701 acgcttataa ttgactccac atttagccac ggaggactaa aaagtattaa atgcttcgct
     8761 caggcaggtg ccgaaggaac agtatcgaaa gcaagtttca gtaaacagaa tatggcattc
     8821 tgggaaggtg aaactatgag agcaagtggc tggtactata taccgggaga taatgaactc
     8881 gactggttgt ttatcatgga tcttgaagaa caaactgcca tcggtgccgg tcccggtatc
     8941 cgacttgcta ttgtagataa ttgtctgatg gttgaacata aatatcttga ggatgatatt
     9001 tatcaaccaa caacatcaca gattccattt ccaagaaatg aatgggtgta tctgaattgg
     9061 gaagtgtatt tatctacaaa agacgacggc accattaaac tgtatcaaaa tggtgaacta
     9121 atactcagca acaatcatac tgtaacctta ccgcaagaca tcctctattt tcagcaaggc
     9181 accaaaggaa tgtatagcag cattgaattt ggcgctacag caaatcctac cggcgctgat
     9241 gcaattgttt gggtggatga catttttgtg gaggtggtca aataattatc cctcagaaaa
     9301 tcacctaccg ataaaaatac cgggttttgt ttcgtcacat attttttaca tatttgataa
     9361 aacaaactca tgaaacgccc tgaactcatc gatcttactc tcaaaataac ggggattatt
     9421 atggtaattc aattggtgaa tagtgtcatt caattgatat ttaccatccc acagattaca
     9481 tttatttggc aaggtccgga tgatggtcaa ttttcatatg tcattgctct ttttggcatt
     9541 tatgttttag ggcctcttat ttatgcagta gttatttatc aattgacatt taaatccgac
     9601 aaatgggctc gtcgaatctt acgaccgcat caaatggaag aagaaataaa tctcaagatt
     9661 gaaccacagg ttatgcttaa tgtaagtata ttgattgcat ccatattttt gttgttctgg
     9721 aatatcccta cacttctcat ggcatttggt caatttatcg cctccaatac gcctcgacat
     9781 atggattaca tgcctgttga ttttgataaa aatgggctga ttgagccaat cctcaatatt
     9841 gctgttgcat taattctttt cacatggtct agccctatca gccattatct ggcgaatcgc
     9901 atagttaaag caactccatc ggaagaacaa gaacaaccta aaatcgacga catatgacat
     9961 cacaactttc gcaacaaatc gctaaacaat tccgagatgc acatgatggc gataactgga
    10021 ctgccgtaaa tctgaaaacg ctgttatccg atgttacccg tgacgaagct gttcaacaga
    10081 ttgaaggatt taattccatt gcaactttgg tgaatcatac aggttattat ttagacattg
    10141 tgattcccgt attgcgtggt gcacctttgg tgggaaagga cagtgatagt tttattttgc
    10201 cggatttgtc cgatcaagaa aaatggcagg cattggttga taaggtgttt gctgatgttg
    10261 aagtatttgc cgcactgata gaatcattcc ctgatgaaaa atggtgggag ccgtttaatg
    10321 atggtaaata cggtaattat tatcgcaata ttaccggtat tatagaacac ctgcactatc
    10381 acatggggca aattgctttc ctcaaaaaaa tgctgcgaaa aagttgattg tggtatgtgt
    10441 ttgtttcgcg caaagtcgca aagttactaa gtcgcaaagc ctgcatgcaa actcactcac
    10501 ccactcaccc actgacccac tcgcctcacc ttatcaccac atccgcagta ttgataaaga
    10561 tgtcgtcagc attatacaaa tcaacatggt ataaacctgc ggaggttaaa tcgagctgaa
    10621 agtgaatata atcccaatca ggttgaacgg taattgaaaa tgcatctaca gggtcagtta
    10681 caccttcttt atatacatcg acatatatta aagaggtttg aatagcctta aaatcattat
    10741 cgaggtaggc tttgaaactt gccttaccat tactgcccat ataaaattcc gtagcaattc
    10801 ccataggatt accttcagcg tcaactgagc cgcagaattt taattcagaa tcttcataat
    10861 aataggtgtc gatggttgaa gaaccggaag ttgttggtgc ttcaccttct tgccaaccaa
    10921 tattaaaatt cacagaagcc agctcaacac cgtcgtgcat gatataggcg cgatagttac
    10981 ctgcatccgt aaatttaaaa tcatacacca gccaattttt atcacgagcc ggttctaacg
    11041 gaagcgtttc atatgctgtc aacgtaccgg aattagtcca gtcgtaatcg atgtataaat
    11101 accaggatcc ggatgaaaat ctcgcgtctt gtttgtataa tatataaacg taggaaccac
    11161 ctgatttgat gtcccatgaa ttataaatac cggttgcggt accattatca tcataa
//
LOCUS       DAOQOU010000103        11107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3033_length_11107_cov_5.974303, whole genome shotgun sequence.
ACCESSION   DAOQOU010000103 DAOQOU010000000
VERSION     DAOQOU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3033_length_11107_cov_5.974303"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..671)
                     /locus_tag="PLM90_09525"
     CDS             complement(273..671)
                     /locus_tag="PLM90_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ89655.1"
                     /translation="MQENEISEIVIKEACYVHSAFGPGLLESVYEIALFTRLSQRGLK
                     VRKQVPIPVLLDGVTLEVGFRADLIVEEKLILEIKSVEALHPVVYMQLKTYMRFTGIH
                     VGLVLNFYVNSMKDGIKRYVYQYNGPTLSK"
     gene            complement(1011..2411)
                     /locus_tag="PLM90_09530"
     CDS             complement(1011..2411)
                     /locus_tag="PLM90_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89656.1"
                     /translation="MIRIKFGTDGWRAIIAQEFTTDNVARVSKATADWIKQSSSTPSV
                     VIGHDCRFGGELFTDVAARVFCEEGVKVFVAKDFVSTPMVSFGAHALNAVAGVVITAS
                     HNPPSYNGFKLKSGYGGPTIPEEIAKVENIIPDHHPIPQTSLAEYEAAGKLQWVDLEE
                     MYYQHVVKSFDLDTINNSGIRLGYDAMYGAGQRVALRIFKNIVPLHCDDNPGFHGQAP
                     EPIHRNLIALSELVKNDPTIKCGLANDGDADRIGMYDGDGNFVDSHHIILLLIHYLHK
                     YKGYTGKVVIAFSVSEKVKRLCEHYGLDYEVTKIGFKYICGKMVIEDVLLGGEESGGI
                     AVKGHVPERDGIWCGLLLMEFMAKTGKNLTELIKEVYDITGSFCFDRNDLHLTDALKN
                     SIIENCKTGKYTAFGKYTVDRLEDVDGFKFHLDANSWVMIRPSGTEPLLRVYAESPTR
                     AETDSILSTVKSILLG"
     gene            complement(2517..3371)
                     /locus_tag="PLM90_09535"
     CDS             complement(2517..3371)
                     /locus_tag="PLM90_09535"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ89657.1"
                     /translation="MKKLLLPVMLLVTTVAGAQATLGLNAQKTYDITPADFGLVFDTI
                     RITTDDGASLFGWHLYPGEKNDKSSKKSVIISHDGNGNMANALEQAGKFLGLGFHVFM
                     YDYRGFGKSSDFTVSPKFYIYQQFATDLNATIEYVKKYHALLSIDLYGFGIGGGLSLG
                     VGANNLKVHRIIADNPYTTFETVQTRYEQKTGTRIMMPIGYNVTLLEPKMALEDKGDH
                     LRGILLIIGEQGSIMTQADMMELQAMKKKVTSIYTVKGVDNDKNFSSNRDEYFKQIKA
                     FYDKFPDS"
     gene            3555..4211
                     /locus_tag="PLM90_09540"
     CDS             3555..4211
                     /locus_tag="PLM90_09540"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMZ89658.1"
                     /translation="MVPGLDTYKHKGMRQGLVDLLRDKGISDERVLAAINAVPRHTFM
                     DPAFLGHAYEDKAFQIGEGQTISQPYTVAYMSEWLEVEPGMRILEVGTGSGYQACVLA
                     EMGGNVYSIERFKKLSDTARKQLDILGYQGVKTFFGDGYEGLPELAPFDRIIITAAAK
                     EIPQALLKQLRPGGIMVFPLGNDDDQLMIKLTKTGEYDYRKEVGDNFRFVPMLPGKVI
                     "
     gene            4463..5278
                     /locus_tag="PLM90_09545"
     CDS             4463..5278
                     /locus_tag="PLM90_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89659.1"
                     /translation="MKKMMIVAIATVMSTGLFAQNMPKAGYNGFGFTITGLSNVGFGN
                     YGNTILGNAVISDPAGVVAAGYDTPLNALIPQQAFMYKRYYDNGMASRLQLGINSMSS
                     KTSVSDSTGVPDEYAVTDESLKGFTIGLGLGMEKHMSTEAAKVDPYVGADLLFGFLTG
                     FNYEGTTDVTGDGYSSNSSLTVSYPGGMGLALNAICGFNYFFSDNISIGAEAGLGFGM
                     VKTGGEWTSDFSYTSNVGGTSTTTTSADRGEYSNSMSGVQVNSRGAVNLIVYW"
     gene            complement(5337..5795)
                     /gene="smpB"
                     /locus_tag="PLM90_09550"
     CDS             complement(5337..5795)
                     /gene="smpB"
                     /locus_tag="PLM90_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HMZ89660.1"
                     /translation="MKPAEINIRNKRATFEFEIIDRYVAGLMLRGSEIKSVREGKVSM
                     NDAYCQFRDGELWVKNLNISEYKEATVWQHEPLRMRKLLLTKKELSKLLAKVKERGFT
                     IIPLRLFVSDRGFAKIEIALARGKKVHDKRASIKEKDEKRDLARTMRKYQ"
     gene            complement(5797..6192)
                     /locus_tag="PLM90_09555"
     CDS             complement(5797..6192)
                     /locus_tag="PLM90_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89661.1"
                     /translation="MINPFQIGDKKVFSIVVTHHDVAAFESGIVHEVYSTFCLARDAE
                     WSTRLFVLDMKESDEEGIGTFIEIYHRSPAAVESQVDFTATLLEVNGHEVICSFTATC
                     GERIIAEGRTGQKILKKEKVNRLMASIAK"
     gene            complement(6249..8135)
                     /locus_tag="PLM90_09560"
     CDS             complement(6249..8135)
                     /locus_tag="PLM90_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HMZ89662.1"
                     /translation="MNLGKNHSATGKSLEDVHGTVDTTARGTGWRRFLAFIGPAYLVS
                     VGYMDPGNWATDLAAGSGFGYTLIWVLLMSNLMAILLQSHSARLGIVRGQDLAQASRT
                     SYPPIINIPLYIFAEIAIAACDLAEVLGLAIGLNLLFGMPLLWGVSIAVLDCILLLLI
                     LNFGIRKMEAFIISMVFIMGASFFVEVFMAKPDVGGIVNGFAPSIPSETALYIAIGII
                     GATVMPHNLYLHSALVQTRKFENTNEGKKRALKFNLIDSTLALNLAFFVNASILILGA
                     AVFFSNGLTEVADIQDAHKLLEPLLGSSLAPILFAVALIASGQSSTITGTLAGQIIME
                     GYLNLRITPWLRRLITRLLAVVPAFLVIWLKGEEETGELLILSQVILSMQLGFAIIPL
                     IHFVSDKKKMGSFAIGTITRIASWVTAIIIVSLNIKLVYATISDLLDKPNNWWVYAFV
                     IPVALYGIVLLLYIAIKPFVSSKTYEPKLINTIEPNMEFAIKGAFKKIAVTLDFSSQD
                     TPTIAQAIAAGGKEAEYILIHIVESAGAITFGKDTGDMESRKDFQQLKAYVDELMRLG
                     YKAKAELGYGRPTRAIPEIVKQQEADMLVMGAHGHRGLKDLILGETINTVRHRISIPL
                     LAVK"
     gene            8278..8709
                     /locus_tag="PLM90_09565"
     CDS             8278..8709
                     /locus_tag="PLM90_09565"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ89663.1"
                     /translation="MENQTPLDQVDLSVEQNYELASQGKRFLNFIIDYIIILGIVVAV
                     ILIIGGFSSESGFYLVFYPTFWLYYVVMEGSTGRTIGKFLTGTVVVRNIDLQAPSISQ
                     CMGRSISRFVPFEQFSFLGSQTGWHDNWTNTMVVNVKSLVR"
     gene            complement(8712..9386)
                     /locus_tag="PLM90_09570"
     CDS             complement(8712..9386)
                     /locus_tag="PLM90_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HMZ89664.1"
                     /translation="MKTPLSHTEENYLKAIFKLSELNSSNVSTNAIAEKLSTTAASVT
                     DMLKKLASKKLLHYEKYYGVTLTEKGKKNAKDLIRKHRLWETFLVDKLRFSWDEVDEL
                     AEQLEHIRSEILTDRIDQFLGYPKFDPHGDPIPDKHGNIAYHEDVTLESMKKGESGII
                     VGVIDHSTEFLRYLDKVDLVIHTRIHISEDEEYDQSKTILVNHKHETVISQKVAANLL
                     VRKTGN"
     gene            complement(9419..9781)
                     /locus_tag="PLM90_09575"
     CDS             complement(9419..9781)
                     /locus_tag="PLM90_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMZ89665.1"
                     /translation="MHKRTLVLGASENPERYANKAVRRLNAAGHDVVAFGKTGDHIGD
                     TKIEREWLPWEDIDTVTLYLNPVHQEAYYDKIIALAPKRIIFNPGTENDAFEALAHKH
                     GIETLEACTLVLLSIGEY"
     gene            complement(9819..>11107)
                     /locus_tag="PLM90_09580"
     CDS             complement(9819..>11107)
                     /locus_tag="PLM90_09580"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HMZ89666.1"
                     /translation="FLISHHNTSKNMAEVIRMPRMSDTMTEGTFVEWHKKVGDTVKIG
                     DILAEVETDKATMELENYAKGTLLYIGVEKGQSAKVDQVIAIVGKEGDDVQALLASAG
                     TVAKAEEKAPSTETKQAEATIEVAATTQDQSASNTEDGRIKASPLAKKIAKEKGLDLH
                     AISGSGPEGRIIKKDVEQANPIAVEQTESAKPQINFAPVAGVESYEEVPVSQMRKVIA
                     QRLSQSKFTSPHFYLSISVDMDKAMAARTSMNAVSPVKISFNDLVVKASAMALRRHPQ
                     VNASWLGDKIRFNHHIHIGVAVAINDGLVVPVLRFADQKSLAQLSAETGDLAERAKSR
                     KLQPQDWEGNTFTISNLGMMGIEEFTAIINPPDACILAVGGINQVPVVKNGQVVPGNV
                     MKLTMSCDHRVVDGAVGAQFLKTLKEYLEDPVRMLI"
BASE COUNT         3117 a         2372 c         2300 g         3318 t
ORIGIN      
        1 gataaagcgc agaggtgggg agataaagcg cggagtttgc ggagttagcg gagtttgcgg
       61 tggtggggag atgaagcgcg gagtttgcgg tggtggagct atcatagatt caagatgcaa
      121 cgggtttggt tgatgatgag gcctattttt tttgactaga ttgctgctta tctcaatctc
      181 aactatcata tcattcggca tgggctttat cattggccag tgaagcattt gaagcaaatt
      241 tacaattcct gatagttatt caagcaatca acttacttag aaagtgttgg gccgttgtat
      301 tgatatacgt agcgtttgat accgtctttc atggagttca cataaaaatt caataccaaa
      361 cctacgtgta tgccggtaaa tcgcatatat gttttcaact gcatgtaaac tacaggatgt
      421 aaagcttcaa cagatttaat ttctaaaatt aacttttctt caactataag gtcagcccta
      481 aatccaacct caagggttac gccatcgagc aaaacaggaa tcggaacttg ttttcgcacc
      541 ttcaatccgc gttgcgataa acgagtaaat aaagcaattt catacaccga ctccaataaa
      601 cccggcccga atgcactatg cacataacaa gcttccttga taactatttc cgaaatttca
      661 ttttcctgca tctcacttat ttaaagcaaa aatgcatatt gaaattttat ttacccgata
      721 gtaagcgatt aactcgaata aagattaaat tttgtgctag cttcccttac agggaaaacg
      781 atgctaacgc taccaactcc agaaacaagt taaactccgc taacccagcg attgacaaca
      841 actctgccaa ctccgtcaac ttagcgattt acaacaactc cgcaaactcc gctaactccg
      901 caattgataa caactctgca aactccgcta actccgcgat ttacaacaac tctgtaaatt
      961 ccgcaactaa aactaccatt acggtaattg ccttttaaag ctttaataca tcaacccaat
     1021 aaaattgact ttactgttga aaggatactg tcggtttcag cgcgggttgg ggattcggcg
     1081 tagacgcgga gcagaggttc tgttcctgat gggcgaatca ttacccaact attcgcatcg
     1141 aggtggaatt taaatccatc aacatcttcg aggcgatcaa ctgtatattt tccgaacgct
     1201 gtgtatttgc cggttttgca attttcaatg atgctatttt tcaacgcatc cgtgaggtgc
     1261 aaatcattgc gatcgaaaca gaaagaacct gtaatatcat acacttcttt aatgagctcc
     1321 gtaagatttt ttcctgtttt cgccatgaat tccatcaaca acagtccaca ccagattcca
     1381 tcacgttccg gcacatgccc tttcactgcg ataccgccgg attcttcacc gccgagtaat
     1441 acatcttcaa taaccatttt accacagata tatttaaatc cgatcttggt tacttcgtaa
     1501 tccagtccat agtgttcaca caaacgtttt actttttcgc taacgctgaa tgcgataacc
     1561 actttaccgg tatatccttt gtatttatgc aggtaatgaa tgagcaataa gatgatatga
     1621 tgtgaatcaa caaaatttcc atctccatcg tacatgccaa ttctatcggc atcaccatca
     1681 ttggcgagtc cgcatttaat ggtaggatca tttttaacca actcgcttaa tgcgatgagg
     1741 ttacgatgaa taggttcggg tgcctgacca tgaaatccgg gattgtcatc gcaatgcaga
     1801 gggacaatat ttttaaatat gcgaagcgca acacgttgtc ctgctccgta catggcatca
     1861 taaccaaggc gaatacctga attattgatg gtatctaaat caaagctttt tacaacatgc
     1921 tgataataca tttcttccaa atcaacccac tgcaattttc ctgcggcctc atattctgca
     1981 agagatgttt gcggaatagg atggtggtcg ggaataatat tttcaacttt tgcaatttcc
     2041 tcaggaattg ttggtccgcc gtagccgctt ttcaatttaa atccattgta ggatggaggg
     2101 ttatgactag cagtgatcac cacaccggca acagcattta aggcatgagc gccaaagcta
     2161 accattggtg tagaaacaaa atctttagca acaaatactt tcacaccttc ttcgcaaaaa
     2221 acgcgagctg caacatcagt gaataattca cctccaaagc gacaatcatg gcctattacc
     2281 accgatggag ttgaagaaga ttgcttaatc cagtcggcag tggctttgct tacacgggca
     2341 acattatctg tagtaaattc ttgtgcaatg atagctctcc aaccgtcggt tccaaatttg
     2401 atgcggatca tgatgtgctg tatgtttatt tcagaccaaa gatagataac aaagggcgta
     2461 aaagtaaaag ccaccgacag taactatcgg tggctttaca atattttaat aactcattat
     2521 gagtcaggga atttgtcgta gaatgctttg atttgtttaa agtattcgtc gcggttgctg
     2581 ctgaagttct tgtcattatc tacgcctttc acggtataga tggatgtaac ttttttcttc
     2641 atagcttgta attccatcat atcggcttga gtcatgatac ttccttgttc gccaatgata
     2701 aggaggattc cacgaaggtg atcgccttta tcttcgagtg ccattttagg ttcgagcaag
     2761 gttacattgt atccgatagg catcataatg cgtgtaccgg ttttttgctc gtagcgtgtt
     2821 tgcactgttt cgaaagttgt ataggggtta tctgcaatga tgcgatgtac tttgaggttg
     2881 tttgcaccaa cacccaaaga caatccaccg ccgatgccga aaccatataa atcgatgctt
     2941 aaaagggcgt gatatttttt cacgtattca atggttgcat taaggtctgt agcaaattgc
     3001 tgatagatgt aaaatttcgg gctcacggta aaatcgctgc tttttccgaa tccgcgatag
     3061 tcatacatga acacatggaa tccgagacca aggaatttac ctgcttgttc gagggcgtta
     3121 gccatatttc catttccatc gtggctgata ataaccgact ttttagacga tttatcattt
     3181 ttctcacccg gatataaatg ccatccgaat aagcttgcgc catcatcagt agtgatgcga
     3241 atggtatcga aaaccagtcc gaaatctgcc ggagtgatat cgtaggtttt ttgagcgttt
     3301 aggccaaggg tggcttgtgc gccggcaact gtggtaacca agagcattac cggtaaaagg
     3361 agttttttca tcaattgcat agtgcgattt gctttttgtt taaaaatgaa atgtgcaatc
     3421 caaggattgt caaatttcgt gccttaaaaa taattctccc tacttgcatt ttctgaaccg
     3481 cgcagcgaaa tgtggataat tagtagtgat tagtggaaag taagccaccg aacagttgtt
     3541 tactttgcag cgccatggta ccgggattag acacatacaa gcataaaggc atgcggcagg
     3601 gattggtaga tcttttgcga gataagggaa tcagtgatga gcgggttttg gctgctatca
     3661 atgctgttcc gcgacacaca tttatggatc cggcgttttt agggcatgct tatgaggata
     3721 aagcctttca gattggggag ggacaaacca tcagccagcc gtatacggta gcttacatga
     3781 gtgaatggtt ggaggtagaa cccggaatgc gcattctgga agtagggacc ggtagtgggt
     3841 atcaggcttg tgttttggca gaaatggggg gaaatgtata ctctattgaa cggtttaaaa
     3901 agttatcaga tacggctcgt aaacagctcg atatattggg ttaccagggg gttaaaacct
     3961 tctttggcga tgggtatgaa ggactgccgg aactcgcgcc attcgacaga atcatcatca
     4021 cagcggcggc aaaagagata ccccaggcat tgttaaaaca gctgcgtccg ggaggcatca
     4081 tggtgttccc acttggcaat gacgatgacc aattaatgat aaaactgaca aaaacaggtg
     4141 aatatgatta tcggaaggaa gtgggtgata actttagatt tgtacccatg cttccgggca
     4201 aagtaattta aatatttacc aaggtgtcgt gcatcgttca gccgcgatga gtcatcttgt
     4261 aataagtaaa tgatgtcaca gccggcatca tagtttttaa atcgctgttg ttacacgcca
     4321 atagtgattt tttacacaca caaattttta acgaacggat tagcacaata ccggagttgc
     4381 attatcgtca tggataatgt ggcaatgatt tgcattcaat ttaacccatt tatataacca
     4441 agtaaacaaa acaacaaaca gtatgaaaaa gatgatgatc gtggctattg ccacagtaat
     4501 gagcacgggc ttgttcgctc agaacatgcc taaagccggc tacaatggtt tcggatttac
     4561 cattaccggc ttatcaaacg taggttttgg taactatggc aataccattt taggtaacgc
     4621 tgtaatcagt gaccctgcag gcgttgttgc agcaggatat gatactccac tgaatgcatt
     4681 gatcccgcaa caggcattta tgtacaagcg ttattatgat aacggaatgg ccagtcgctt
     4741 gcaattaggt atcaatagca tgagttcaaa aacttcagta agtgattcta caggtgttcc
     4801 tgatgaatat gcagttaccg atgagtcatt aaaaggtttc acaataggcc ttggtcttgg
     4861 tatggagaaa catatgagca ctgaagctgc caaagttgac ccatatgttg gagccgattt
     4921 acttttcgga ttcttaaccg gatttaatta tgaaggtact accgacgtaa ccggtgatgg
     4981 ttatagttct aacagcagcc tgacagtgtc atatcccggc ggtatgggac ttgcattaaa
     5041 tgcaatctgc ggattcaatt atttcttcag cgataatatt tctatcggtg ctgaagcagg
     5101 tcttggattt ggtatggtga aaaccggtgg tgagtggaca agcgatttca gctataccag
     5161 taatgtcggt ggcacgtcaa ctactactac atctgccgat cgtggcgaat acagcaattc
     5221 aatgtcaggc gtacaggtga acagtcgcgg cgctgtgaac ttaatcgttt attggtaatt
     5281 catttctctt atgaataaaa aatcccggtc aatgccggga ttttttattt tattatttat
     5341 tgatacttgc gcatggttct ggcaaggtct cgtttttcat ctttttcttt gatagatgcg
     5401 cgtttatcat gtactttttt tccgcgggcc aaggcgattt caatttttgc gaatcctctg
     5461 tcgctcacaa ataatctcaa tggaattatg gtaaatccac gttcctttac ttttgccaga
     5521 agttttgata attctttttt agtgagcaat aatttccgca tgcgaagagg ttcatgctgc
     5581 caaaccgttg cttctttgta ttcggagata tttaaattct tcacccacaa ttcaccatcg
     5641 cgaaattggc aataggcatc attcatgctt acttttcctt cgcgaacact tttaatttct
     5701 gagccacgca acattaatcc ggcaacatac ctgtcgatga tctcaaattc gaaggtggcg
     5761 cgtttatttc tgatatttat ttctgcgggt ttcatctcat ttggcgatgg atgccatcag
     5821 cctgtttact ttttctttct ttaaaatctt ttgtcccgtt ctgccttcag caataatgcg
     5881 ttcaccgcag gtagcagtaa atgagcaaat aacttcatga ccattaacct cgagcaaggt
     5941 agccgtaaaa tcaacttgcg attccaccgc tgcgggagag cgatgataga tttcgatgaa
     6001 ggtacctata ccctcttcat cgctttcttt catgtcaagc acaaacaatc ttgtactcca
     6061 ttcagcatcg cgggcaaggc aaaaggtact atacacttcg tgtacaatac cactttcaaa
     6121 tgcggccaca tcatgatgtg ttacaacaat tgaaaaaacc tttttatcac caatttgaaa
     6181 tgggttaatc acgcggtaaa gatacacaag cctttgttac tgcgcgatat ttaaccggtt
     6241 gaagcttatt attttacggc taacaatggg attgaaatac gatgcctcac ggtattgatc
     6301 gtttcaccaa ggatcaaatc ttttaatcct ctgtgaccgt gagcacccat tacgagcatg
     6361 tcggcctcct gctgtttcac aatttcggga attgcgcgtg ttgggcgacc gtaacccaat
     6421 tcagctttgg ctttatatcc taatcgcatc aactcatcga cataggcctt tagttgttgg
     6481 aaatcttttc tggattccat atcaccggta tccttaccaa atgtgatggc accggcgctt
     6541 tcaacaatat gaataagaat gtattccgct tcctttccac ctgctgcaat tgcttgagca
     6601 atagtaggtg tatcctgaga tgaaaaatcg agggtcactg caattttttt gaaagcacct
     6661 ttaatggcga attccatatt tggctctatg gtattgatca gtttaggttc atacgtttta
     6721 gaactgacga aaggttttat tgcaatatac aataacaaaa caatgccata taaagccacc
     6781 ggaatcacaa aggcatatac ccaccaatta ttcggtttat ccagcagatc gctaatggta
     6841 gcatacacga gtttaatatt caaactcaca ataattattg cagttaccca agaagcaatt
     6901 cgcgtgattg tgccgatagc gaaagagccc attttctttt tatcgcttac aaaatgaata
     6961 agcggtataa tggcgaaacc aagttgcata ctcaaaataa cctgtgaaag gatgagcagt
     7021 tcaccggttt cttcttcacc cttcagccaa atcacaagaa atgccggcac tacagccaga
     7081 agcctagtta tcaatcttcg caaccaaggg gtaatgcgca gatttaaata tccttccata
     7141 ataatttgtc ctgccagagt gccggtaatg gtagaacttt gtccggaggc gatcaatgca
     7201 acggcaaaca aaataggtgc aagcgatgat cccaataagg gctctaacaa tttatgcgca
     7261 tcctgaatat ctgcaacctc tgttagtccg ttagagaaaa atactgcggc acctaaaatc
     7321 aatattgaag cattaacaaa aaaagcaaga ttcaatgcta acgtactatc tataagatta
     7381 aattttagtg cacgtttttt cccttcgttt gtattttcaa atttccgcgt ttgcaccagg
     7441 gcactatgta aatacaaatt atgaggcatt acggtagcac ctatgatacc gatggcgata
     7501 tataaagcag tttcagaagg tattgatggc gcaaatccat ttactattcc accaacatca
     7561 ggctttgcca tgaacacttc cacaaaaaac gaagctccca taatgaacac catcgatatg
     7621 atgaaagctt ccattttgcg gatgccgaaa ttgaggataa gtaacagcag tatacaatct
     7681 aaaacagcaa tagacacccc ccataaaaga ggcattccaa ataagagatt tagcccgata
     7741 gccagtccaa gcacctcagc aaggtcacag gctgcaatgg ctatttcagc aaaaatgtac
     7801 agtggaatat tgatgatagg cggataacta gttctgctgg cctgagcaag gtcctgtccg
     7861 cgaacaattc cgagtcgcgc gctgtgactc tgcaaaagga tggccataag attgctcatc
     7921 aggaggaccc aaatcaaggt atagccaaag ccactcccgg cagcaaggtc ggtagcccaa
     7981 ttgcccggat ccatgtagcc aacgcttacc aggtacgcag gaccaataaa cgccagaaag
     8041 cgcctccagc ctgtgccacg ggcagtggta tctaccgtac cgtgcacatc ttccaacgat
     8101 ttacctgtgg cgctatggtt tttccctaaa ttcatttcaa atgcctgatt ttaaggcaaa
     8161 gataaaatat tttttagatt agtctaaaat ttattttaaa accccaaaat atgctttttt
     8221 gttccgttta gattttggca gttggaagaa aattgtattt ttattgaaaa tacactcatg
     8281 gaaaaccaaa caccccttga tcaggtagat ctgtcggtag agcagaatta cgaactcgca
     8341 tcacaaggca agcgatttct caatttcatc attgattaca tcatcatttt aggaatagta
     8401 gttgctgtta ttctaattat tggaggattt tcaagtgaat caggatttta cctcgttttc
     8461 tatcctacat tctggcttta ctatgtggta atggaaggat ctaccggcag gactatcggc
     8521 aaatttttga caggcacagt ggttgtcaga aatattgatc ttcaggcccc gagcatcagt
     8581 caatgtatgg gaagaagtat ttcacgcttt gtaccgtttg aacaattttc ttttttggga
     8641 agtcaaaccg gatggcatga taactggacc aatacgatgg tagttaatgt caaatcattg
     8701 gtgagataag attaattacc tgtctttcta acaagcaggt ttgccgcaac tttctgagaa
     8761 atgaccgttt cgtgtttatg attcacgaga atggttttgc tttggtcata ttcttcgtct
     8821 tcggagatat gaatacgtgt atgaatcaca aggtctactt tatcgaggta gcgtagaaat
     8881 tctgtgctgt gatcgataac accaacaatg ataccggact cacccttctt cattgactcg
     8941 agtgtaacat cttcgtggta ggcgatattg ccatgtttat cgggaatggg atccccatgc
     9001 ggatcgaatt tcggatatcc gagaaactgg tcaatacgat cggttaaaat ttcggagcga
     9061 atatgttcca attgttctgc aagttcatct acttcatccc aactaaagcg caatttatct
     9121 acaaggaaag tttcccaaag cctgtgtttg cgaatcaggt cttttgcgtt cttttttcct
     9181 ttttctgtaa gcgtaacccc ataatatttc tcgtaatgca ataatttttt tgaagccagt
     9241 tttttcagca tgtcggttac cgatgcggca gtagtgctta atttttcggc aatggcgtta
     9301 gtactcacat tgctgctatt cagctccgac aatttaaata ttgccttgag gtaattttct
     9361 tcggtatgag aaaggggtgt tttcaaatca gctgcatgaa tgccgttagt gaacaatttc
     9421 aatattctcc tatcgacagc aaaactaagg tacaggcttc aagagtttca atgccatgtt
     9481 tatgcgctaa ggcctcgaaa gcatcgtttt ccgttccggg gttgaaaatg atacgtttgg
     9541 gagccagggc aattatttta tcatagtagg cttcctgatg cacagggtta agatataatg
     9601 ttacggtatc aatgtcttcc cacggcaacc attctctttc gatttttgta tcgccgatat
     9661 gatcgccggt tttcccgaag gccactacat catgaccggc ggcatttagt ctccgcacag
     9721 ctttattagc atagcgctca ggattttcac tagccccaag aacaagcgtt cgtttatgca
     9781 taataataaa atgtaattgg caacgaaata gttcagcctc agatgagcat acgcaccgga
     9841 tcttccagat actccttcaa ggttttgagg aactgagcac caactgcccc atcaaccact
     9901 cggtgatcgc agctcatagt cagtttcatt acatttccgg gaactacttg tccgttctta
     9961 acaacaggaa cctgattgat accgccaacc gccaaaatac aagcatcagg aggattaatg
    10021 atggcagtaa attcttctat tcccatcatt ccaaggttag agatggtaaa tgtatttcct
    10081 tcccaatcct gtggctgcaa ttttctgctt tttgcgcgtt ctgcaagatc gccggtttct
    10141 gctgataatt gagccagtga tttctggtcg gcaaaacgaa gcaccggcac taccaggcca
    10201 tcattaatgg cgacagcaac accgatatgg atatgatgat tgaatctaat tttatcgccc
    10261 agccagctag catttacttg agggtgacgt cgcaacgcca ttgctgatgc tttcactacc
    10321 aagtcgttga atgatatttt aacagggctt acagcattca tgctggtgcg tgctgccatt
    10381 gccttatcca tatctacact gatcgacagg tagaaatgtg gtgaagtgaa tttgctttga
    10441 gaaagacgtt gtgcaataac ttttcgcatt tgcgacacgg gcacttcttc ataactttcg
    10501 acaccggcta ccggagcgaa attaatttga ggttttgcag attctgtttg ttcaacagca
    10561 atcggatttg cctgctcgac gtctttttta atgatacggc cttccggacc tgaaccggag
    10621 attgcatgca ggtcgagtcc tttttctttg gctattttct ttgccagtgg cgaagcttta
    10681 attcttccgt cttctgtatt cgaagcagat tgatcttgtg ttgtggctgc aacttcgatt
    10741 gttgcttcag cctgtttagt ttctgttgaa ggcgcctttt cttccgcctt ggctactgtt
    10801 cctgcagaag caagcaaagc ctgaacatca tcaccttcct taccaacgat ggcgatcacc
    10861 tgatccactt ttgcgctttg acccttttcg acaccgatat acaacagtgt tcctttggcg
    10921 tagttttcaa gctccatggt agccttatcg gtttccactt cagccaggat atcgccaatt
    10981 ttcacggtat cgcccacttt cttatgccat tccacgaagg tgccctcggt catggtatcg
    11041 ctcatacggg gcattctgat cacttctgcc atattctttg aggtattatg gtgcgaaatt
    11101 aagaaac
//
LOCUS       DAOQOU010000104        11106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3034_length_11106_cov_6.275088, whole genome shotgun sequence.
ACCESSION   DAOQOU010000104 DAOQOU010000000
VERSION     DAOQOU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_11106_cov_6.275088"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3150
                     /locus_tag="PLM90_09585"
     CDS             <1..3150
                     /locus_tag="PLM90_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89667.1"
                     /translation="TISDYNQSNFAIGAEEQYNIGGQTKIIGNKVFIYFDGNHQHLRK
                     QVREGVGKVLINSMIFGGNLQEIVQNAVLLNLPEWFTEGLVSYIGEEWNTELDNQLRD
                     GILSGRYQKFNKLTGEDARFAGHALWYYIAESYGESTIPNLLYLIRINRSIENGFLFV
                     LGTGVNGTIDAWYNYFLTKYQKESAGKTLPALENAIPRKERKEVQFHQLALSPDGEHI
                     AYAMDDHGKFKVFMQEMDTKDRQKIKSGGLKTHTLATDLSNPLIAWSPDGKKLAIVYE
                     KRYKNYITTYDTEKKEKETKELTRFQKILHISFAKSSSKLVMSVVNNGQSDLYMYTLT
                     NDRVEQLTNDYYDDLDPVYVQLNNGYEGVIFASNRDLDTIFTTRAIDSILPVGNMDLY
                     FYDYNRKSKDLVKITHTRRISESAPLQFDGKHFAYLSDQNGIINRFGGYLDSLYDHTD
                     TLVYYPDSTVTNPPYAYKDFTLFPKYDSVLLADIYRDTAYVFPVSNYASNILEHSINW
                     KTGAMVETFKSGKSTVFLKAQAPSDIGALQQNALDNTTFRQFIDQKKKGDDVVMTINE
                     KDTSSLITHQTIITKTDTIHKEIDEGMYFQTDFPEPDPETKLLSEPVTLGNSANPEMQ
                     NINTGKKFKSSRITPYRLKFSSDYVTSQLDNSLFINKYESFNTTGGVYSTPNLGALLT
                     VSISDLFEDYRFVGGMRFPTSFSGGEYFLTYEDLKHRLDKKFTWYYQTDNQQYTFQPT
                     WFPLVDAGLSTNIVEAAFKWPIDVVRSVRGTVSYRAENITFLANDTFSLNLPDYSEDW
                     LSVKFEYVHDNTWPVMTNIMNGMRYKVWLEMHKQFNVDPNADVAVDFSEGYLGVLGFD
                     VRHYQKIHRQIIWANRIAGGTSFGKQKLIYYLGGVDSWIAPNFNNDIEVNTDMGYAFQ
                     TLATNLRGYDQNIRNGNSYAVLNSELRFPVFSYFFNKPVRSEFLRNLQVVGFYDIGSA
                     WEGFSPFEEDNPYNDATITQQPITVVVNYFRDPLVMGTGLGMRTTLFGYFIRVDRAQP
                     IEAGGLGDPKWYFSLSLDF"
     gene            3338..4534
                     /locus_tag="PLM90_09590"
     CDS             3338..4534
                     /locus_tag="PLM90_09590"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HMZ89668.1"
                     /translation="MSTIHNKIWWRNTNERNVGITDRKISWLELFYDLVYVIAISRAT
                     HHLSAHLTFEGFLDYFYMVALIYWGWLNGSWHHDILGANDVRTIFLTIWQMVILSALI
                     ATLGTGEEMNLFNVTIAILLMQLYISYLWWSEGIYDKTNKDVRLIYVGFYMLTFALIV
                     STLFIDPAYLRVVFYTALMINYIPPYFVTRRASHELRYEQRLSPSMTERLGLFTIILF
                     GEVILGVINGATAFEKLTAYEWSVFVAAIFMVFSIWYIFFKLIGDKHVKKGYVNSNLY
                     AFLFLPIAIALGFTGVSFENVLAAGHAHHTNVNTELFLGLSIALFLISIVLLTLLVES
                     KQVYVNAMPKMRRLLILVIAGIVVTSIISNSFSLGEFLMILLILLLILPVMILVLWNN
                     LELKKQ"
     gene            4635..5360
                     /locus_tag="PLM90_09595"
     CDS             4635..5360
                     /locus_tag="PLM90_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HMZ89669.1"
                     /translation="MPTTQNKIEELKETGFTTVESLYSDTEIEQIIMAISHADSSKET
                     FRKSNDLFAIRQFLKEIPEVANYIFNDKLKKIISEYFGTNYFVVKSIYFDKPETSNWY
                     VAYQQDLTISVNQKIEIENFGPWTIKQNQFAVQPPIEFLENMYTIRIHLDDTDENNGA
                     LRVIPKSHVKKIYRPETIDWTTENESICKVAKGGIMIMKPLILHSSGRTTNNNKRRVI
                     HIEFSNTELPDGLHWAERMNVLN"
     gene            5700..6614
                     /locus_tag="PLM90_09600"
     CDS             5700..6614
                     /locus_tag="PLM90_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ89670.1"
                     /translation="MKPNVKAHIYVLAANIIYGINYSVGKVALKEMSPLAIVAFRVCC
                     AFLVFFAAHQIFIKENVDRADHKKFILASLFGVAVNQMLFFTGLSLTSEMHASLIMIT
                     TPLLVLIMAWVILRDPITITKSIGILSGAAGVALLIISGTVADNSTASAFGDLCIFIN
                     ATSYAIFLVIAKPLMKKYAPLTLAKWIFFYGMFMVIPFGFNDLQQLSFGEISTQAWLA
                     LSVVVFGATVLAYLFNILGLRYGSPALVSIYIYTQPIIATMVALMLGTDSLTLPKVIS
                     AILVFAGVALVSRSSIAIPAAEPEAKLK"
     gene            6640..7512
                     /locus_tag="PLM90_09605"
     CDS             6640..7512
                     /locus_tag="PLM90_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HMZ89671.1"
                     /translation="MLISMTGYGRTTFALNGKNIVLEVKTLNSKSFDLNLRIAPLFKE
                     YEMEIRNLLSKYIIRGKTDCLLTYEQMDEKAGEININAIKEYYRQIKSISDDMGLEQA
                     NIMDLVFRLPNITTSSNEPLTDEQWEMVTNHIDNTCGQLTKFRTVEGINLEQDLILHV
                     NLIHSALIEVEALDPKRMAQQREKMHNDLRSYLASENIDMNRFEQEVIFYLEKMDITE
                     EVIRLRSHCEYFMQTIEDKAQEKGKKLGFIAQEMNREINTIGSKAYHADMQRKVVEMK
                     DELEKIKEQLNNVL"
     gene            7512..8081
                     /gene="gmk"
                     /locus_tag="PLM90_09610"
     CDS             7512..8081
                     /gene="gmk"
                     /locus_tag="PLM90_09610"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMZ89672.1"
                     /translation="MSGKLIIVTAPSGAGKTTIVRHLLAVEPLLAFSVSATTRQKRDY
                     EKDGVDYHFLSADQFRKQIEENAFVEWEEVYPGKFYGTLRSEVERMWAAGKHIIFDVD
                     VKGALSLKSKFPEKTLSLFISPPSVEILAQRLIDRGTETPSTLQTRLERVRYELTFSP
                     KFDHVIVNDVLETAKTEAQRLVHNFLNMA"
     gene            8130..8510
                     /gene="apaG"
                     /locus_tag="PLM90_09615"
     CDS             8130..8510
                     /gene="apaG"
                     /locus_tag="PLM90_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HMZ89673.1"
                     /translation="MVTSITQGVKVSVETFYQQEFANKGEYVFAYRITIENKSEHTVQ
                     LLRRHWYIYDGIGTWREVEGEGVVGETPVIAPGHIHQYVSGCHLLAPAGKMHGSYLMM
                     RTLDGMYIDVTIPEFHMVAPFVLN"
     gene            complement(8557..9615)
                     /locus_tag="PLM90_09620"
     CDS             complement(8557..9615)
                     /locus_tag="PLM90_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89674.1"
                     /translation="MEYKHKRFLAVAVMGVLLAWGLSSCDKGYKTPHPDISHVPEQQV
                     SIVRIEQLLHDADTSNYASVYDEMQKKYPDIYNSLTENFWGLVESDSMSRRQIYDSLY
                     RNTSGNVWMQRLFDSVMLVYTDLDDVEKDLSQAYRYFKYYFPDSSLPQLYSYIGPFVY
                     WTIFDSTTLGIELDMYLGKHFGYYGSYESNMPQYISLRCDKPYIAVNVMQSLVDGMVP
                     SLGPNGTLLDEMLRQGKILYYLDCVMPDVEDSIKIGFASGKVEWCYDNEGEIWKFLAG
                     EDLLFSTRTDDHRRYLGEAPSSMNMPDDSPGRAAVWSGWQIVRSYMEEHPKTTLQQLF
                     FEMDGMRLLKESNYDPGE"
     gene            9653..10321
                     /locus_tag="PLM90_09625"
     CDS             9653..10321
                     /locus_tag="PLM90_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ89675.1"
                     /translation="MKKIMLAAVFISALALSARAQDFRFGLTASPVFDWTRVEGSLFE
                     GTGTKVGFQYGLLFDKTIGSVERYAFSSGILINYASSGVSYTDTITDIKSSWNASVQY
                     IEIPLTMRLRTNEVNYITYYGQFGLTPGICIKARGNTVVDNVTILDDVNLRDDANATL
                     ATYNLMNISLTLGAGAEYSLSENTSIMGGVFFQNGFANIIKDDVDDNGSTLKQIGIRL
                     GVLF"
     gene            10382..>11106
                     /locus_tag="PLM90_09630"
     CDS             10382..>11106
                     /locus_tag="PLM90_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMZ89676.1"
                     /translation="MRIALAQINVHIGNFDANFQKMAAHIEEAKKLHADIILFPELSV
                     CGYPPRDFLDFSDFVDKCYEVIEKIKPLTNDIAVVVGSPSRNPRIEGKDLHNSAFFIF
                     EGEIKHIVNKALLPTYDIFDEYRYFEPATEFANFNFKGRRLALTVCEDLWNVGNDNPM
                     YKGCPMDILALDPEKPEVMLNISASPFDYNHAAERIETLKANTRKYGIPVFYVNHCGA
                     QTDLIFDGGSLVINANAVVYDEMP"
BASE COUNT         3394 a         2412 c         2145 g         3155 t
ORIGIN      
        1 actatttcag attacaacca aagtaatttc gccattggag ccgaagagca gtataatatt
       61 ggagggcaga cgaaaataat tggcaataaa gtatttattt atttcgatgg caatcatcag
      121 catctgagaa aacaggtgcg cgaaggtgtg gggaaagtgc taatcaatag catgatattt
      181 ggcggcaact tgcaggaaat cgttcaaaac gccgtactgc tgaatttacc tgaatggttt
      241 accgaaggcc ttgtgtctta tattggcgaa gagtggaata cggaacttga taatcagctg
      301 cgcgatggca ttttatccgg cagatatcaa aaattcaata agctgaccgg tgaagatgcc
      361 cgattcgcag ggcatgcttt gtggtactat attgccgaaa gttatggcga atcaactatt
      421 ccgaatttgt tgtatctcat tcgcattaac agaagtattg agaacggatt cctatttgta
      481 ctcggcacag gagtaaacgg cactattgat gcatggtaca attatttcct caccaaatac
      541 caaaaagaat ctgccgggaa aacattacct gcacttgaaa atgcgatacc cagaaaagaa
      601 cgcaaggaag tgcaatttca tcaactggct ttaagccccg acggcgagca tattgcctac
      661 gccatggatg atcatggtaa gtttaaagta ttcatgcagg aaatggatac caaggaccga
      721 cagaaaatta aatccggtgg attaaagaca catacgcttg ctactgatct cagcaatcca
      781 ctgattgcct ggagccccga tggtaaaaaa ctcgctatcg tttatgaaaa gagatataag
      841 aattatatca ccacttacga taccgaaaaa aaggaaaaag aaacgaagga actaacacgt
      901 ttccaaaaaa tattgcatat cagctttgcg aaatcatcct caaaattggt gatgagtgta
      961 gtcaataacg ggcaaagcga tctctacatg tacaccttga ccaacgacag ggttgaacag
     1021 cttacaaatg attattatga tgatctggat cctgtgtatg tgcaattaaa taatggctat
     1081 gaaggtgtca ttttcgcgtc aaacagagac ctggatacta ttttcaccac aagagccatc
     1141 gattcgattt tacctgtggg caatatggat ttgtatttct atgattacaa tcgcaaatcg
     1201 aaagatcttg tcaaaatcac ccatacacgt cgcatcagcg aatcagcacc tttgcaattc
     1261 gatggcaaac attttgcata tctgagcgat caaaatggta tcataaaccg gtttggaggg
     1321 tatctggata gtttatatga ccataccgat acattagttt attatcccga cagcacagtt
     1381 accaatccgc catatgctta caaagatttc acgctattcc cgaaatacga ttctgtttta
     1441 ttggcagata tttatcgcga tacagcatat gtgtttcctg tgagcaacta cgcttccaat
     1501 attcttgagc atagcatcaa ttggaaaacc ggtgctatgg tggaaacatt taaatcagga
     1561 aaatcaactg tatttttaaa agcacaagca ccgtctgata ttggagcatt gcaacaaaac
     1621 gctctcgaca atacaacttt ccgtcaattt atcgatcaaa agaaaaaggg cgacgatgtg
     1681 gtgatgacca ttaatgaaaa ggatacaagt tcattgatca cacatcagac catcatcaca
     1741 aagaccgata ccatacacaa ggaaattgat gaaggcatgt atttccagac agacttcccc
     1801 gaacctgatc ctgaaacgaa attgctttct gaaccggtta cacttggcaa ttcggctaac
     1861 cctgaaatgc aaaatatcaa tacaggaaaa aaattcaaat catcccgcat cacaccttat
     1921 cgcttgaaat tttcaagtga ttatgtgaca agtcagttgg ataatagttt atttattaac
     1981 aaatacgaaa gcttcaatac gacaggcgga gtatattcga caccaaacct cggagcattg
     2041 ctcacggtca gcatcagcga tctgtttgaa gattaccgtt ttgtaggcgg catgcgcttt
     2101 ccaacatcat tttcgggtgg tgaatatttt cttacctatg aagatttaaa acatcgtctg
     2161 gataaaaaat tcacgtggta ttatcagaca gataatcagc aatatacttt ccaacctaca
     2221 tggttccctc tggttgatgc cggtttatca accaatatcg ttgaagctgc attcaagtgg
     2281 ccgatagatg tggtgagaag tgtacgcggt actgtttctt atcgcgcaga aaatattacc
     2341 tttttagcca atgacacctt cagtctcaat ttaccggatt attcagaaga ttggttgtcg
     2401 gtaaaatttg aatatgtgca tgataatacc tggccggtaa tgaccaatat catgaatggc
     2461 atgcgatata aagtatggct ggaaatgcac aaacaattta atgtagatcc gaatgccgat
     2521 gtggctgttg atttcagtga aggttatctt ggtgtgttgg gatttgatgt gcgtcattat
     2581 caaaaaattc atcgccagat catttgggcc aatcgcattg caggaggcac ttccttcgga
     2641 aaacaaaaat tgatctatta cctcggtggt gtagatagct ggatagctcc gaatttcaat
     2701 aacgatattg aagtaaatac cgacatggga tatgcttttc agaccttggc gacaaatttg
     2761 cgcggttacg atcagaatat tcgcaatgga aacagctatg cggtattaaa ttcggaattg
     2821 cgtttccctg ttttctctta cttctttaat aagcctgtgc gttctgaatt tttgcgcaat
     2881 ttacaggtgg ttggatttta tgatatcggt agtgcatggg aaggattttc tccttttgaa
     2941 gaggataatc cttataacga tgccaccatt acgcagcagc cgataacagt tgttgtaaat
     3001 tatttccgcg acccattggt tatgggaaca ggcttaggaa tgcgcactac ccttttcgga
     3061 tacttcattc gcgtagatcg cgctcaaccc atagaagccg gcggcctggg tgatccaaaa
     3121 tggtatttct ctctcagttt ggatttttga ttgtaagtgg gtttatggtt tgtgctattt
     3181 ttcgcgctaa gacgctaaga tgcaaagccg caaagcttta actcacattc accactcacc
     3241 attcaccatt cacccactca ccactcacca ttcacccctc acccctttga attccccacc
     3301 acatcccata cttttacaat tgctcaaata gcattcaatg tcaactatac acaacaaaat
     3361 atggtggagg aataccaatg aacgaaatgt tggtattacg gatagaaaaa tcagttggct
     3421 ggaattgttt tatgatttag tgtatgtgat tgcaatatcc agggcaactc accatttatc
     3481 ggcacatctg acatttgaag gattcttgga ttacttttat atggttgctc tgatttattg
     3541 gggttggctc aatggcagct ggcatcatga tattcttgga gcaaatgatg tacggacaat
     3601 tttcctgaca atatggcaaa tggtcatatt atcagcacta atagctaccc ttggaacagg
     3661 cgaggagatg aatttattta atgtcaccat tgctatatta ttgatgcaat tatatatttc
     3721 atatttatgg tggagtgaag gcatttatga taaaacaaat aaagatgtca gattaattta
     3781 cgtaggcttc tacatgctga cctttgcgct gattgtcagc acactgttta ttgatccggc
     3841 atacctacgc gttgttttct atacggcctt aatgatcaat tacattccac catacttcgt
     3901 aactcgaagg gcaagtcacg agcttcgata tgaacaacgc ttgtctccaa gtatgacaga
     3961 acgcctggga ttgtttacga taattttgtt tggtgaagtg attctcggtg ttataaatgg
     4021 agcgacagca tttgaaaaat tgacagcata cgaatggtcg gtattcgtgg ctgcaatatt
     4081 tatggtgttt tcgatttggt atatattctt caaattgatt ggcgacaagc acgtaaaaaa
     4141 gggttacgtt aacagcaatt tgtatgcatt tctatttctg ccgattgcaa tcgcattagg
     4201 attcacaggt gtatcgtttg aaaatgtgtt ggccgcaggt cacgcacatc atacaaatgt
     4261 aaatacggaa ttatttttag gattatcaat tgcgctgttt ctcatttcta ttgtgttgct
     4321 aacactgctg gtagaatcta agcaggtata tgtaaacgct atgcccaaaa tgaggcgttt
     4381 gcttattttg gtcatcgccg gaatagtagt gacatcaata atatcaaaca gcttttcatt
     4441 gggcgaattc ttaatgattt tactgattct actcctcatt ttaccggtta tgatattggt
     4501 gttgtggaat aatctggagt taaaaaaaca ataacaccaa tcaacttcct aatatgtatc
     4561 ccgtctgaaa cgttgggcac tttaatttca cttctctatc tgcacaacac cactactcaa
     4621 tccataacca caaaatgccc acaacccaaa acaaaattga agagctcaaa gaaacaggat
     4681 ttacaactgt tgaaagcctc tattccgata ctgaaatcga gcaaataatt atggctattt
     4741 cacatgccga cagcagtaaa gaaactttta gaaaatcaaa cgaccttttt gcaatcagac
     4801 aatttttaaa ggaaattccg gaggtagcga attatatatt caatgataaa ttgaaaaaaa
     4861 tcatctccga atattttggc acaaattatt tcgttgtcaa aagcatttat ttcgacaagc
     4921 ctgaaacttc gaattggtat gtggcctatc aacaggatct aactatttca gttaatcaga
     4981 aaattgaaat agaaaacttt ggtccatgga caataaaaca aaatcagttt gccgttcaac
     5041 caccaataga attccttgaa aacatgtata ccatccgcat tcaccttgat gatacagatg
     5101 aaaacaacgg tgcacttaga gtaatcccaa aatcgcatgt aaaaaagata tacagacctg
     5161 aaaccatcga ctggactaca gaaaacgaaa gcatttgcaa ggttgccaaa ggaggaataa
     5221 tgatcatgaa acctttaatt ctgcatagtt caggaagaac cacaaataat aataaaagac
     5281 gtgttatcca tatcgaattt tcaaacactg aacttcctga cgggttgcat tgggcagagc
     5341 gaatgaatgt attaaattaa acgctcaatc gaaacattca caacctaata ctaataaaca
     5401 accaaccccc aatattcaac tcactatacg tatcttgtaa atggggtgag tgggtgagta
     5461 tcgtgaagcg tgagtcgtga ctcgtgaata taaataataa gtaactacaa tatagatttc
     5521 ctccttcaaa ccttcaaagc tttaatcctt cgaatctttg aatcttcaaa ccttcgaatc
     5581 ttcaatcctt caatccttcg aatcttcaat ccttcaaccc ttcaaatctt caattcttca
     5641 aaccttcgaa tcttcaattc ttcaaccctt caaatcttca aatcccgtac ctttgccccg
     5701 tgaaacccaa cgtcaaggca cacatttatg tgcttgcagc aaacattatt tatggtataa
     5761 attatagtgt cggaaaagtg gcacttaaag aaatgtcgcc tctagcaatt gtagcatttc
     5821 gtgtctgttg cgcctttctg gtgttttttg ctgcacatca gatttttatc aaagaaaatg
     5881 ttgaccgcgc agatcacaag aaatttatcc tcgcaagtct ttttggtgtt gccgtaaatc
     5941 agatgttgtt tttcaccgga ctcagtctta ccagcgaaat gcatgcctct ctgatcatga
     6001 ttactacgcc attgttggtg ctcatcatgg catgggtaat tttgcgcgat cccattacca
     6061 tcaccaaatc aattggcatt ttgtcgggcg ccgctggtgt tgcattactt attatttcgg
     6121 gaactgttgc cgacaattca acagcttcgg cattcggcga tctgtgcatt tttatcaatg
     6181 ccacttccta tgccatattt ttagtgatcg caaaaccatt gatgaaaaaa tatgccccat
     6241 tgacactggc aaagtggatt ttcttttatg gcatgttcat ggttattcct ttcggcttca
     6301 acgatcttca acaattatct ttcggtgaaa tatctacaca ggcctggtta gcactttcag
     6361 tggttgtttt cggggcaacc gtgctggcat atttattcaa tattctcgga ttgaggtatg
     6421 gcagccctgc actggtgagc atttacattt atacccaacc cattatcgca accatggtag
     6481 cccttatgct tggaacagac agcctcaccc ttccaaaggt tatttctgcc atcctggtat
     6541 ttgccggtgt cgcattggta tcgcgcagtt ctatagccat tcctgcagca gaacctgaag
     6601 ccaaattgaa ataagctaac tttacacaaa attttgtgta tgctcatttc aatgacagga
     6661 tatggtagaa caacatttgc gttgaacgga aagaatattg tgctggaagt aaaaacactc
     6721 aatagcaaat ctttcgacct caacctgcgt atcgcccctc tgtttaagga atatgaaatg
     6781 gagatccgca acctgctgag taaatatatc atcagaggaa aaactgattg cctgcttact
     6841 tacgaacaaa tggatgaaaa ggcaggagag ataaacatta atgctattaa ggaatattat
     6901 cgtcagataa aatccatttc cgacgacatg ggtcttgaac aggctaatat aatggacctt
     6961 gtttttcgct tgccaaatat caccacctct tcaaatgaac cactcaccga cgagcagtgg
     7021 gaaatggtaa ccaatcatat cgataatact tgtggtcagc tcacaaaatt ccgcacagtc
     7081 gaaggtatta atctcgaaca agatctcatc ctgcacgtca acctcattca cagcgcttta
     7141 atagaagtag aagcactcga tccgaaacgc atggcacagc agcgcgaaaa aatgcacaac
     7201 gatctgcgct cttatctcgc atcggaaaat attgatatga accgtttcga acaggaagta
     7261 attttctatc tcgaaaaaat ggatatcacc gaagaagtga ttcgcctccg ctcacattgc
     7321 gaatatttta tgcagactat tgaagataaa gcgcaggaaa aaggtaaaaa actcggattc
     7381 attgctcagg aaatgaatcg cgaaatcaat actataggca gcaaagcata tcatgccgac
     7441 atgcaacgca aggtagtaga gatgaaagat gaattggaaa aaataaaaga gcagctcaac
     7501 aacgttttat aatgagtggc aaactgatta tcgtaacagc tccatcaggc gcaggaaaaa
     7561 cgaccatcgt cagacacctg ttagcggtag agcctttgct tgcattttca gtttctgcaa
     7621 ccactcgcca gaaacgcgat tatgaaaaag atggcgttga ttaccatttt ttgagtgccg
     7681 atcaattccg caaacaaatt gaggaaaatg cttttgtgga gtgggaagaa gtttatcccg
     7741 gcaaattcta cggcaccctg cgcagtgaag tagaacgcat gtgggcagca ggaaaacata
     7801 tcatcttcga tgtggatgtg aaaggtgccc tcagcttaaa atccaagttt ccggaaaaaa
     7861 cactgagtct gtttatcagt ccgccgagtg tagaaattct agcccaacgc ctcatcgaca
     7921 gaggcacaga aactccctca accctgcaaa cacgcctcga aagagtgcgt tatgagctca
     7981 ctttcagccc gaaattcgat catgttattg tgaacgacgt gctggaaacg gcgaaaacag
     8041 aggctcagcg cctcgtgcat aactttttga acatggcatg agcgcctaac cccatttttc
     8101 tgttacattt acatcaccaa ccttaaggca tggttaccag cattactcaa ggcgtaaagg
     8161 tcagcgtaga aactttctac caacaagaat ttgccaataa aggcgaatat gtgttcgcat
     8221 atcgtatcac tatcgaaaat aaaagcgagc ataccgtgca attacttcgt cgccattggt
     8281 atatctacga tggtatcgga acctggcgtg aagttgaggg cgaaggtgtt gttggtgaaa
     8341 ctcctgttat cgctcccggt catatccatc aatacgtttc cggatgtcac ctgctggcac
     8401 cggcaggcaa aatgcatggc tcctacctca tgatgcgcac cctcgacggc atgtacatcg
     8461 atgtcaccat cccggaattt catatggtgg caccttttgt gctgaattaa tttcttccaa
     8521 tatttttccg gtatccgtca agtaatgatt catccatcat tcccccggat cataattcga
     8581 ttccttcaac aatcgcatgc cgtccatttc aaaaaataat tgctgcaatg ttgtcttggg
     8641 atgctcctcc atatacgatc gcacaatctg ccagccactc caaacagccg cacgacccgg
     8701 actgtcgtca ggcatattca tagacgaagg cgcttctcca agatatctcc ggtgatcgtc
     8761 ggtgcgcgta gaaaataata aatcctctcc cgcaagaaat ttccatatct caccttcatt
     8821 atcatagcac cattccacct tgcctgatgc aaaaccgatt ttaatggaat cttcaacatc
     8881 cggcatcaca caatcgagat agtataaaat ttttccttgt cgcaacattt catcgagcaa
     8941 tgtcccattc ggaccgagcg atggcaccat cccatcaacc aaactctgca tcacattcac
     9001 agcaatatac ggcttatcac atcgtaaact gatatactgc ggcatattac tttcgtaact
     9061 gccatagtaa ccaaagtgct tccctaaata catatccaac tctattccca aagtagtact
     9121 gtcgaatatt gtccaataca caaacggacc tatataagaa tataattgcg gcaagctgct
     9181 atccggaaaa taatatttaa aatatcgata agcctgcgat aaatcttttt ctacatcatc
     9241 caaatcagtg tataccaaca tcacactatc aaacaaacgc tgcatccata catttccgga
     9301 agtgtttcga tataatgagt catagatctg cctacggctc atggagtcac tctccaccaa
     9361 tccccaaaaa ttttctgtga gcgaattata gatatcagga tactttttct gcatttcatc
     9421 ataaactgat gcatagtttg aagtatctgc atcatgcaac aattgttcta tgcgaacaat
     9481 cgaaacctgc tgctccggga catgcgaaat atccggatga ggggtcttat aacccttatc
     9541 gcaactgctc aatccccaag ccagcagcac acccattacc gcaaccgcta aaaaacgctt
     9601 atgtttatat tccatgtatc acctatcttt ggcactgtaa aactaaaaag ccatgaagaa
     9661 aattatgctt gcggccgtgt ttatttctgc actggccttg tctgcccggg ctcaggattt
     9721 ccgctttggg ctcaccgcct ctccggtttt cgactggacc agagtggaag gatcattgtt
     9781 tgaaggcacc ggaacaaaag taggcttcca gtatggttta cttttcgata aaactatcgg
     9841 cagtgttgag cgctacgcct tttccagcgg catcctcatc aactatgcaa gtagtggtgt
     9901 ttcttatacc gatactatta ccgatatcaa aagttcatgg aatgcatcgg tgcaatacat
     9961 cgaaattcct ctcaccatgc gtttgcgcac caacgaagtg aactatatca cctattacgg
    10021 tcaattcgga ctcacccccg gcatctgtat caaagcacgt ggaaacacgg ttgttgataa
    10081 tgttaccatt ttggatgatg tgaacctgcg cgacgatgca aacgctaccc tggcaaccta
    10141 taatctgatg aatatcagcc tcaccctggg cgccggtgcc gaatattcgc tgtctgaaaa
    10201 tacatctatc atgggtggtg tattttttca gaacggtttc gccaatatca ttaaagacga
    10261 tgtcgatgat aacggctcta ccctcaaaca aatcggtatc cgtctaggcg tattattcta
    10321 ataccaggct aacttattat atttaacttt gtcacgtcat ataacggcgt gacatttttt
    10381 tatgcgtata gccctcgctc aaattaacgt gcatatcgga aatttcgatg ccaattttca
    10441 aaaaatggca gcacatatcg aagaagcaaa aaaattacat gccgatatca tcctcttccc
    10501 cgaactcagc gtgtgcggat acccaccacg tgattttctc gacttctccg attttgtcga
    10561 taaatgttat gaagtcattg aaaaaatcaa accacttact aacgatatcg ctgttgttgt
    10621 cggctcacca agcagaaatc cgcgtatcga aggaaaggat ttacataaca gcgcattttt
    10681 catttttgag ggagaaatta agcatatcgt aaataaagca ctcctgccta cttacgacat
    10741 tttcgatgag tacagatatt tcgaaccggc gacggagttc gcaaatttca atttcaaagg
    10801 tcgccgcctg gcactcaccg tttgcgaaga tctatggaat gtaggcaacg acaatcccat
    10861 gtataaaggt tgtcccatgg atatacttgc actcgatccg gaaaaacccg aagtgatgct
    10921 taacatcagc gcatctcctt tcgattataa tcatgctgca gagcgcattg aaacactgaa
    10981 agcaaataca cgtaaatatg gcatccccgt attttatgta aatcattgcg gcgcacaaac
    11041 cgacctcatc ttcgatggcg gtagtctggt tatcaatgcc aacgctgttg tatacgacga
    11101 aatgcc
//
LOCUS       DAOQOU010000105        11082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3042_length_11082_cov_6.029836, whole genome shotgun sequence.
ACCESSION   DAOQOU010000105 DAOQOU010000000
VERSION     DAOQOU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3042_length_11082_cov_6.029836"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLM90_09635"
     CDS             <1..340
                     /locus_tag="PLM90_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HMZ89677.1"
                     /translation="LQKETVAQMRPGTVIVDLAASTGGNCELTENGKTININGVTIIG
                     DSNLASTMPQDASKMFGKNILNFLKIMIDKDGNTALNFDDDLITGTCLAHEGNIRSER
                     VKAQMSTVTA"
     gene            361..678
                     /locus_tag="PLM90_09640"
     CDS             361..678
                     /locus_tag="PLM90_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMZ89678.1"
                     /translation="MILLDIFQNFLASLDTWDIIYIVLLAAFLGVEVISHVPAILHTP
                     LMSGANAIHGVVIIGSIIVMGHAESTLALVLGFLAVILGTLNVVGGFVVTNRMLQMFK
                     KKK"
     gene            809..2200
                     /locus_tag="PLM90_09645"
     CDS             809..2200
                     /locus_tag="PLM90_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMZ89679.1"
                     /translation="MTTLDILKLCYLLGSVSFIVGLKMLAKPDSAKRGNMLAAIGMGI
                     AIIGTILFHRNEEDESIGNLAWIFGGIAIGTVVGYLAAMKVKMTQMPQMVSLFNGMGG
                     ACAALIGIVEMDTHNPEVFGERFTIFLGLMIGTVSFAGSIIAYGKLEGKVKDFGSKFQ
                     QWFNNTFIFAILALLVYASLTPDFMPSSVIWIIFGASMLYGILFVLPIGGADMPVVIS
                     LLNSFTGVAAAFGGFLYHNQVMLTGGILVGSAGTILTVLMCNAMNRSLWNVIIGAFSG
                     SSGGGKSTEGLSIREINVTDTAILCAYAQKVTIIPGYGLAVAQAQHACHEFEKVLEDK
                     GVEVNFAIHPVAGRMPGHMNVLLAEADVPYDKLKEMEDINPHLPNTDVVIVIGANDVV
                     NPAAETDPSSPIYGMPIIKANTAKNIIVMKRSMAKGYAGIENELFYDNKTRMLFGDAK
                     ATLEKLVAEVKNV"
     gene            complement(2211..2447)
                     /locus_tag="PLM90_09650"
     CDS             complement(2211..2447)
                     /locus_tag="PLM90_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89680.1"
                     /translation="MKSTGTCPKCNSTKLYTNAGLAKRGDRSMIALSSWKGLYVDVYV
                     CSQCGFIEEYASTRELENPTTLEKLKTNWKKVNE"
     gene            2561..3796
                     /locus_tag="PLM90_09655"
     CDS             2561..3796
                     /locus_tag="PLM90_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ89681.1"
                     /translation="MAQPTKKMVVIIGGGAAGFFCAINLARLRPDLEVHILEKSQQVL
                     SKVKVSGGGRCNVTHACFNPEELIENYPRGGKELLGPFHHFQPADTIQWFEDRGVELH
                     TEDDGRMFPVTNSSQTIIDCFLREANKYHVHIHFGVQVESITSVDNAWKIDCKNQTFH
                     AIAVMMATGSSQQAWSLLKQLGHTIIPPVPSLFTFHIRDKRIDGLMGVSIPHVQASLM
                     NTDLITEGPLLITHWGLSGPAILKLSAWGADVLAKKQYRASIQINFCPTYDSASCIDY
                     LRGMRNDHPKKKVVNDHFPTIPARLWKQLCAFGNITENHNWADLSNIMIESFAEILTS
                     STLSINGKSTFKEEFVTCGGIALNEVNMRNMESRLHPNLYFGGEMLNIDAVTGGFNFQ
                     AAWTTGWLAARSIAGNQTS"
     gene            3859..5379
                     /locus_tag="PLM90_09660"
     CDS             3859..5379
                     /locus_tag="PLM90_09660"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ89682.1"
                     /translation="MHIRTTLLALGFASVAMAQNPGDNIFNTNQVLTIDLQFSQPGFW
                     DSLVANYATETYMMADLTVTDNTGTYTFDSVGVRLKGNSSYGHPGNKKSFKIDFNRYV
                     AGQEYDGLRKLNFNNCFKDPSFMREKIYYDICKADSIHAPRANYADVYMNDELWGFYS
                     VVEQVDDEFLKTHFDENNENLFKAGDAFGGGPGGGVEGDLKYYGTVQDDYYDRYTLEN
                     NTDENDWTDLINLTDFINNSSDADFAGQLSHHFDVEQLMKCMVMYNMFSNLDSYLNSA
                     RNYYIYDYDSTGIWEWINWDCNESFGLYTGGPGTGDLQELDIYYYNLNRPLFERVLTI
                     EDTKTQYETYYCELFNAYFNNSYISDLIDELYTLIQPHVYADPNKMYTNDQFETNIES
                     DIAGGMGGTTYGLKSFVTNRSAYIASALDCSTQTDISTNTAFDVHIFPNPTSDMIYIN
                     GDFTSPCKLAVYGLDGLVLYQLDQVQLPTSISLANQPSGMYLVVVGEQQTIITKQQ"
     gene            complement(5512..5997)
                     /locus_tag="PLM90_09665"
     CDS             complement(5512..5997)
                     /locus_tag="PLM90_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89683.1"
                     /translation="MHTRIFTFMILCAMATAVKAQNNELHAVGIRFGGGDGVGAEISF
                     QQYIGGPNRLEFDLGAFEDGYNDGFKLTVLYQWVHSIDNSFYWYAGLGGGLGARDHDN
                     GHPFEGDDDDGVFVDFDFDLGMEYNFDFPLQLSLDIRPEIGLINDDFDMGWALGVRYR
                     F"
     gene            complement(6091..9846)
                     /locus_tag="PLM90_09670"
     CDS             complement(6091..9846)
                     /locus_tag="PLM90_09670"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ89684.1"
                     /translation="MKLFYSVALCLLGLFSFPITISAQMMDDCAAHPDDDVMHTEHCA
                     VFALVPEDAATHVSIANGSWFSSATWNTGTVPGNEARVLVDSGFTVTYDAVATAAIEW
                     IRVNGTLNFSTTVNTQLRVTDFIIDPLGHLNIGTVAAPIPNGVTAKILFSDLGPIDYM
                     DDPYRFGKGLISHGFLNVNGYYKKTFCAVTKNLNAGTNNIKLAETPTGWQIGDQLVVP
                     GTYGTAYGDFDANTKFHDEVVTITSIAGKTIYFTNNATGTSILQYEHKLPAGYGLKMY
                     VANLTRNVVFESENYNVIPDDQRGHVMLMHNIGASVMNAQFNGLGRTNKDILATDPVV
                     DEFGNLISGGENVRGRYALHVHRAGTNNIAAIPVQIKGCSVVAATSWGVVNHESNVNA
                     DDNVVFDYFGGAFVTEDGNELGTFNRNIAIKGRKATTVTDLTTRTLNIDFAFEGNGFW
                     IQSSNVGYENNIVTSSAGDAYKVFSDDASMPAEYRVKIPKENILNPIIAGADDSIYTA
                     VVPLRKFSGNIAYNCNSAMMFWTHMLNNDNVGDFSTIAYDPYTHTIMSVIDGCKFWNM
                     LGAGISVKYSGQVHFKNTILLGDLTNQYQTSDWIAGNPYGGYAFITSTVTGQIIYEGL
                     TVKGWKRGVVAGRTDDLQSGDTKEYDYRTSKIIGGTYAANLYNIVPEEGTDVYGASEY
                     YKFPDYFEISGSPSFTAITPNVLPTPDFAYLSAGGNAVQFNGALSLDTDPGVITPGQG
                     NGIASYVWNFGDGSSGYGKDPVHTYNLPGTYSVTLTVYDSQGKTGVLTRNVYVSTTQY
                     SNAVANSGFETGGLTTASYINSTRAFVDNGWIHKSNWQLLAGKATILLSDKWNRPLVQ
                     VIKNDKVLRGNVSFSFQAKNLGIGAIGNDLYAEIVGVNGEFKDMNFTSLGAVTKWNLN
                     DVDVVPTVLYSENLGLAEYNWQTFTRTVNFGSGYDYIIVKFYSQGVKTGPGESQGIDN
                     VCLPCVCAVPGGLFADALTSSHATLIWDNVGSTQYQVQYKTTAGATWTTVTVENTYLD
                     LSSLSANTSYTWKVKAWCSGAWTAFSGDNIFITPSAGTTCTSPSELSTELITPNKATL
                     VWNAIPGALQYQVSYKSLLGLTWTDVTTTNTTLSLTGLLPLTAYNWKVKTQCAAGWKD
                     YTTLLTFTTLPLREGEEIGENDLNIQAWPNPVSYELQLTFSEEISGTGSLRLIDISGQ
                     IVKAIDIQIDGTNIAIPVNVSDLPTGIYVLQVALINGQMFNTKIIKE"
     gene            complement(10026..10445)
                     /locus_tag="PLM90_09675"
     CDS             complement(10026..10445)
                     /locus_tag="PLM90_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ89685.1"
                     /translation="MARIQLTFPEKTLFTTVLDVRITDLNYGGHLGNDSVLSLLHEAR
                     VRFLQYLGFRELDVAGTGIIMSDAAIQFKGEAFYGEQLTIAMAVADFSRVSFDLYYLV
                     TNNDKTIALAKTGIVFFDYTQRKMVPVPEAFLLAVNR"
     gene            complement(10462..>11082)
                     /locus_tag="PLM90_09680"
     CDS             complement(10462..>11082)
                     /locus_tag="PLM90_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMZ89686.1"
                     /translation="CLTVSGGHTQIVRVNSAMDMAILGQTIDDAAGEAFDKCAKMLGL
                     PYPGGPLIDKYAAQGNAQAFDFGEPKIQGLDFSFSGFKTSVLYFLQEQTKKNPDFVKN
                     NLNDLCASVQRGIVEILLRKVKRAAKETGISRIAISGGVSANTALRKNLTDMGVALGW
                     EVFIPKFEYCTDNAAMIAITGYQKFLAGAFARYDVVPMARIPGIYI"
BASE COUNT         3288 a         2413 c         2179 g         3202 t
ORIGIN      
        1 tcttcaaaaa gaaacggttg ctcaaatgcg tccgggaact gtaattgttg acctggcagc
       61 aagcaccggt ggcaattgtg aacttacaga gaatggtaaa accatcaata tcaatggcgt
      121 gacaattatt ggcgatagca atctcgcttc taccatgccg caggatgcaa gtaaaatgtt
      181 tggtaaaaac attctcaact tcctcaaaat catgatcgac aaagacggta atactgcttt
      241 gaattttgac gacgatctaa ttaccggcac ttgcttagca catgaaggca atattcgcag
      301 cgaacgtgtg aaagcacaaa tgtcaaccgt aactgcctaa ccaaaaaaga attatcactt
      361 atgatactgc tcgatatttt tcagaatttc ctcgccagtc tcgacacctg ggatatcatt
      421 tacattgtgt tgcttgcagc atttcttggt gttgaagtga tcagtcacgt accggcaatc
      481 ctccacacac cactgatgtc gggcgctaac gccattcacg gtgtagtaat catcggatcc
      541 attatcgtga tgggtcatgc cgaaagtact ctggcactgg tgctcggatt cctcgccgtt
      601 atcctcggaa ccctcaacgt agttggcggc ttcgttgtta caaatagaat gttgcaaatg
      661 tttaaaaaga aaaagtaaat gtggttgtgt gttcatgtgt ccatgtgttc atgaatatgc
      721 attatcctaa ttacaactca ccaatcacca ttcacaaatc accacccgcc tatcggcgag
      781 gcaagctcac cattcaccac tcaccaatat gacaactcta gatatactta aattatgtta
      841 cctcctcggc agtgtttctt tcattgtcgg attgaagatg ttggcaaaac ctgattctgc
      901 taaacgcgga aatatgttgg ctgccattgg tatggggatc gccattatcg gaacgatttt
      961 atttcacaga aatgaggaag atgaaagcat aggtaacctc gcatggatat tcggtggtat
     1021 cgccattggt actgttgttg gttacctggc agccatgaaa gtgaagatga cacagatgcc
     1081 gcagatggta tcgctattca atggaatggg tggagcttgt gcagccttaa ttggcattgt
     1141 tgaaatggat acgcataatc cggaagtatt cggtgagcgc tttactattt tcctgggatt
     1201 gatgattgga acagtttctt tcgcaggttc tatcattgct tatggaaagc tcgaaggaaa
     1261 agtaaaagat ttcggttcta aatttcaaca gtggtttaat aatactttca ttttcgccat
     1321 tcttgcttta ttggtttatg catcattaac acctgatttt atgccatcaa gtgttatatg
     1381 gatcattttt ggtgcttcca tgttgtatgg tattttgttt gtattgccta tcggtggtgc
     1441 agatatgccg gtggtgatat cattactcaa ctcatttaca ggtgttgccg ccgctttcgg
     1501 tggtttctta tatcataacc aagtgatgtt aaccggtggt atccttgtag gttctgccgg
     1561 taccattctt accgtattga tgtgtaatgc catgaaccgt agtttgtgga atgtgatcat
     1621 cggtgcattt agtggaagtt ccggtggtgg taaatcaact gaaggtctga gcatccggga
     1681 aataaatgtt accgataccg ctattctttg tgcttatgcg cagaaggtaa ccatcattcc
     1741 gggttatggt cttgccgttg ctcaagcaca acatgcgtgt cacgaatttg aaaaggtgtt
     1801 ggaagataaa ggtgttgaag tgaattttgc tattcaccct gttgccggtc gtatgccagg
     1861 gcatatgaac gtgttgcttg cagaagcaga tgtgccttat gataaattga aagagatgga
     1921 ggatatcaat cctcacctgc caaatacgga tgttgtaatt gtgatcggtg ccaatgacgt
     1981 ggtaaaccct gctgcagaaa cagatccgtc atcacctatt tatggcatgc cgatcatcaa
     2041 agcgaacacc gcaaaaaata ttatcgtaat gaaacgttcc atggcaaaag gttacgccgg
     2101 tatcgagaac gaattattct acgataataa aacgcgcatg ctctttggtg atgcaaaagc
     2161 aacactcgag aaactggttg ctgaagtgaa gaacgtctga tcaatattga tcattcgttt
     2221 acttttttcc agttggtttt cagcttttct aatgtagtgg gattttccaa ttcgcgtgtg
     2281 gaagcatatt cttctataaa cccgcattgg gaacaaacat acacatccac atacaaccct
     2341 ttccagctac ttaatgctat catgctgcga tcgccacgct tggctaaccc tgcattggtg
     2401 tacaacttag tgctattaca ttttgggcat gtacctgttg atttcatgat tgcaatttga
     2461 ttacgaatat aagccaattt tctgcctcat tatccaacta accaacacgg gattcgttaa
     2521 tataggaagc attcaataat accatatacc tttgtcgtac atggcacaac ccactaaaaa
     2581 aatggtagtg ataataggtg gaggcgcagc aggattcttt tgtgcaataa atcttgctcg
     2641 cttgcgtccc gaccttgaag tgcatattct tgaaaaatcg cagcaggtgt tgagcaaagt
     2701 aaaagtttcg ggtggggggc gatgtaatgt tacgcacgca tgttttaatc ccgaagaact
     2761 tattgaaaat tatcctcgcg gagggaagga actgctcggg ccttttcatc attttcaacc
     2821 tgcagatacg attcaatggt ttgaagatcg aggtgtggaa ttgcatactg aagatgatgg
     2881 tcgcatgttt cctgtgacca attcttccca aacaattatc gattgttttc ttcgtgaagc
     2941 aaataaatat cacgtgcata ttcattttgg tgtgcaagtt gaatccatta cctcagttga
     3001 taatgcatgg aaaattgatt gcaagaatca aacatttcat gccattgcgg ttatgatggc
     3061 aaccggcagc agtcagcaag cgtggagttt attaaagcaa cttggtcata ccattattcc
     3121 acctgttcca agtttattca cttttcatat tcgcgataaa cgcatcgatg gattaatggg
     3181 agttagcatt cctcatgtgc aggcatcgct tatgaataca gaccttatca ccgaaggacc
     3241 attactgatt acacattggg gattgagtgg tccggcaata ttaaaacttt ctgcatgggg
     3301 agctgatgtt ttggcgaaaa aacaatatcg cgcaagcata caaatcaatt tttgcccaac
     3361 atatgatagc gcatcatgca tcgattattt gcgtggaatg cgaaacgatc atccgaaaaa
     3421 gaaagtagtg aatgatcatt tcccaactat tcctgccaga ttatggaagc agctttgtgc
     3481 atttggcaac ataaccgaaa atcacaactg ggcagatttg tcaaacataa tgatagaaag
     3541 ctttgctgaa atattaacct catccaccct tagcatcaac ggcaaaagta ctttcaagga
     3601 agaatttgtt acctgtggag gtattgcgct caacgaagtc aatatgcgca acatggaaag
     3661 ccgactacat cccaacctat atttcggtgg cgaaatgttg aatatagatg ctgttaccgg
     3721 aggattcaac ttccaggcag cttggacaac agggtggctg gcggcaaggt caatagccgg
     3781 aaatcagact tcctaaaaaa cccggtttta caattaaacc ttttttccca aagaccgtca
     3841 taccacaaat ttcacagcat gcacattaga accaccctgc tggcattagg ttttgcctct
     3901 gtagctatgg cacagaatcc cggcgacaat atctttaaca ccaatcaagt actgaccata
     3961 gatctgcaat tctctcagcc cggattctgg gatagtctgg tcgccaacta tgcaacagaa
     4021 acatatatga tggctgatct cactgttacc gataatacag gcacctatac cttcgacagt
     4081 gttggtgtgc gattgaaggg aaattcaagt tatggtcacc cgggaaataa aaaatcattc
     4141 aaaatagatt tcaatcgtta tgtagcaggg caagaatatg acggcttgag gaaattaaat
     4201 ttcaacaact gctttaaaga cccaagcttt atgcgtgaga agatctatta tgacatttgt
     4261 aaggccgaca gcattcatgc accgcgagct aattatgctg atgtatatat gaacgatgaa
     4321 ttatggggat tttattccgt cgttgaacaa gtagatgatg aatttttgaa aacacatttt
     4381 gacgagaaca atgaaaatct ttttaaagca ggtgatgcct ttggtggcgg acccggtggc
     4441 ggtgttgaag gagatttgaa atattatggt actgtgcagg atgattatta cgacagatat
     4501 acgttagaga acaataccga tgaaaacgac tggacagatc tgatcaacct cactgatttc
     4561 atcaataatt cttccgatgc tgattttgcc ggacagctat cacatcattt cgatgtagaa
     4621 caattgatga aatgcatggt gatgtataat atgttcagca atctggatag ctatctcaat
     4681 tctgcacgca attattatat ctatgattac gacagcacag gcatttggga atggatcaac
     4741 tgggactgca atgagtcatt cggtctttac accggcggcc ccggaaccgg tgatctgcag
     4801 gaattagata tatattacta taatctgaac cgacctttat tcgagcgcgt gcttacaata
     4861 gaagatacga aaacacagta cgaaacgtat tattgcgagt tgttcaatgc ctatttcaac
     4921 aatagttata tcagcgattt gatcgacgag ctatatacac tcattcaacc gcatgtttat
     4981 gccgatccga ataagatgta tactaatgat cagtttgaaa cgaatattga atcagatatt
     5041 gctggcggaa tgggtggcac cacgtatgga ttaaaatcct tcgtcaccaa tcgcagcgca
     5101 tatattgcaa gcgcactgga ttgcagtaca caaactgata tatcaacaaa tactgcattc
     5161 gatgtacaca tttttccgaa tccaaccagc gatatgatat atatcaatgg agatttcaca
     5221 tcaccatgca aactggctgt ttatggactt gacggacttg ttttatatca gcttgatcaa
     5281 gtacaattac ccaccagcat ttcactcgct aaccaaccgt caggaatgta tctggttgtt
     5341 gtgggagaac agcaaacaat cataacaaag cagcaatgat caaatactaa tttcattaag
     5401 taaaagaaaa cgcgatagta actcacgcac tgtgcatact atcgcgttta taattgtata
     5461 aatacctcgg agcaatgatg aacattaacc attccacctt gctgtccacc attagaaccg
     5521 gtatcgcaca ccgagtgccc agcccatatc aaaatcatcg ttgatcaatc cgatctcagg
     5581 gcgaatatcg aggcttaatt gcaatgggaa atcaaaattg tattccattc cgaggtcgaa
     5641 atcaaaatca acaaaaacac catcgtcgtc atcgccttca aacggatggc cattgtcatg
     5701 atcccgagcg ccgagcccgc ctccaagacc ggcataccaa taaaatgaat tgtcgatgga
     5761 atgcacccat tgatagagaa cagttaattt aaatccatcg ttatacccat cttcaaaagc
     5821 gccaaggtca aattccaacc tattgggacc tccgatgtat tgctgaaaag aaatttcggc
     5881 acctacacca tcgccaccac cgaaacggat acctactgca tggagttcat tgttttgtgc
     5941 cttgacagca gtggccatcg cacaaaggat cataaatgtg aaaattcgag tatgcatgtt
     6001 gttggtttta atcatgaatg ataaataaca gaaaataaaa agcccgatat cgcactgata
     6061 ccgggccgct attcataaaa gaaccacctt ttattcttta atgatcttcg tgttaaacat
     6121 ttgcccgttt atcaaggcaa cttgcaacac ataaattccg gtcggtaaat cagatacatt
     6181 taccggtatc gcaatgtttg tgccatctat ctgaatatca atggctttaa caatttgtcc
     6241 ggaaatgtca attagtcgta aactgcctgt accactgatt tcctcgctaa atgtcaattg
     6301 caattcataa gataccggat ttggccatgc ctgaatattg aggtcatttt ccccaatttc
     6361 ttcgccttca cgcaacggta gtgtagtgaa tgtcaacaat gtagtataat ctttccatcc
     6421 ggcagcgcat tgcgttttta ccttccaatt atatgcagtt aatggtaaaa gaccggttaa
     6481 tgataaagta gtatttgttg ttgtaacatc tgtccatgtc aatccaagca atgatttata
     6541 tgaaacctga tattgcaatg ctcccggaat ggcattccaa actaacgttg ctttattggg
     6601 tgtgatcaac tccgtagata attcgctagg cgatgtacaa gttgttccag ccgaaggtgt
     6661 aataaaaata ttatctcccg aaaatgctgt ccatgcacca ctgcaccaag ctttcacctt
     6721 ccatgtgtat gatgtattag ccgaaagtga agaaagatcg agataagtat tttctaccgt
     6781 tactgttgtc cacgtagctc ctgctgttgt tttatattgc acctgatatt gcgtgctacc
     6841 cacattatcc caaatcagtg ttgcatgaga agaggtcaat gcatcggcaa acaatcctcc
     6901 cggaacagca caaacacacg gtaaacaaac attatcaatg ccttgtgatt caccgggtcc
     6961 ggtttttaca ccttgcgaat aaaatttcac aatgatataa tcatatccgc ttccaaaatt
     7021 cacggtcctg gtaaatgttt gccagttgta ttcggcaaga ccgaggtttt cactgtaaag
     7081 cacagtagga actacatcca catcattcaa attccattta gtcacagcac ccaacgaggt
     7141 gaaattcata tccttgaatt cgccattgac gccaacaatc tcagcgtata agtcattgcc
     7201 aatggcgccg atgcccaaat tttttgcctg aaaactaaaa gacacatttc cacgcaatac
     7261 cttgtcattt ttaattacct gcaccaaagg tctgttccat ttatcgctca ataaaatggt
     7321 ggctttccct gccaataact gccaattgct tttgtgaatc catccgttat cgacaaatgc
     7381 acgcgtgcta ttgatataac ttgccgttgt taatccacct gtctcaaagc cggaattagc
     7441 taccgcgttg gaatattgtg tagtagaaac atatacattt ctggtaagta caccagtttt
     7501 tccttggcta tcataaacag tcaatgttac actgtaagtg ccgggtaaat tataagtatg
     7561 cacaggatct tttccataac cgctgctgcc atcaccaaaa ttccaaacat aagatgcaat
     7621 gccattgccc tgtccgggag ttataacccc cggatcagta tcaagcgata aagcgccatt
     7681 aaattgcacg gcatttccac cggcagataa ataggcaaaa tccggtgtcg gcaatacatt
     7741 tggtgttatt gccgtaaatg aaggactacc actgatctca aaataatcag ggaatttata
     7801 atactcagaa gcaccgtata catctgttcc ttcttcaggc acaatattat ataaattagc
     7861 ggcgtaagtg ccacctatta tttttgaagt tctatagtcg tactctttgg tatcaccact
     7921 ttgcaaatca tcagttcttc ctgcaacaac accacgtttc caacctttaa ccgtaagacc
     7981 ttcgtaaata atttgtccgg taacagtact ggtaataaaa gcatatccgc catagggatt
     8041 tcctgctatc cagtcgcttg tttgatactg attggttaaa tcgccgagca aaatagtatt
     8101 tttaaaatgt acttgtccgg aatattttac agaaataccg gctcccagca tattccagaa
     8161 tttacatcca tcaattacac tcataatagt atgggtatat ggatcatagg caattgtcga
     8221 gaaatcgccc acattatcgt tattcaacat atgtgtccaa aacatcattg cgctgttgca
     8281 gttgtaagca atattcccac taaatttcct caatggcaca acagcagtat aaatcgaatc
     8341 atctgcaccc gcaataatcg gatttaatat attttcctta ggaattttca cacgatattc
     8401 agcaggcata gatgcatcat cactaaatac tttataggca tcacctgctg agctggtaac
     8461 gatattattt tcgtaaccaa cattcgaact ctgtatccag aatccattcc cttcaaatgc
     8521 aaagtctata ttaagtgtcc gtgtggtcag atcagttacc gttgttgcct ttctaccttt
     8581 tatggcaatg tttcgattaa atgtacccag ttcatttcca tcttctgtaa caaaagcacc
     8641 accgaaataa tcaaacacca cattatcatc tgcattgaca ttgctctcat gattcacgac
     8701 accccagctg gtagccgcca ctacagaaca acctttaatc tgaacaggaa tagctgcgat
     8761 attatttgtt cctgccctgt gcacgtgtaa tgcatatctg ccgcgcacat tttcccctcc
     8821 actgatcaaa ttgccaaatt catctactac aggatctgtt gccagaatat ctttattcgt
     8881 cctacctaag ccattgaatt gcgcattcat caccgatgcg ccgatattat gcatcaacat
     8941 gacatgtcca cgctgatcgt cggggattac attgtaattt tctgattcaa acaccacgtt
     9001 ccttgttagg ttggctacat acattttcag tccataccct gcaggaagct tgtgctcata
     9061 ttgcaatatg cttgttcctg tggcattatt tgtaaaataa attgttttac ctgcaataga
     9121 agttatagtt accacttcat catggaattt cgtgttcgca tcgaaatcgc cataagcagt
     9181 tccgtaagtg ccgggaacaa ctaactgatc gccaatctgc caacctgttg gcgtttctgc
     9241 aagcttaatg ttattggtgc cggcattcag atttttcgta acagcgcaga atgtcttttt
     9301 ataatatcca tttacattca aaaaaccatg acttatcaat ccttttccaa accgataagg
     9361 gtcatccata taatctattg gcccaaggtc gctgaaaaga attttcgctg taacaccatt
     9421 cggaattggc gctgcaactg ttccaatatt taaatgcccc agcgggtcta taataaaatc
     9481 agtaacacgc agttgggtat ttactgtagt tgaaaagttc aatgtaccat ttacacggat
     9541 ccattcgata gcagctgtgg caacggcatc gtaagtaact gtaaaaccgc tatctacaag
     9601 cactcttgct tcgtttcccg gaaccgttcc tgtattccat gtagcagatg agaaccatga
     9661 tccattggca attgaaacat gtgtagcagc atcttccggc accaatgcaa atacagcaca
     9721 gtgctcggta tgcatcacat catcatcggg atgggcggcg caatcgtcca tcatctgagc
     9781 agaaatagta ataggaaaag aaaataagcc aagcaggcac aacgccacgg agtaaaacaa
     9841 cttcatacgc acacaaacaa ttagaggtta agaaatacgt gtgggtttcg ggcctcttgt
     9901 gtgtatttgc cggagaggtg tgtgctaacg aagcggaaaa acgaaataca aatcaatatt
     9961 aaaatttcca tggcaacaac atcatgctgc acgaaaattt gtgtattgtc taaaatgatg
    10021 gagaattatc tgttcacagc aagtaaaaat gcttcgggca caggaaccat tttacgttgt
    10081 gtatagtcga aaaaaacaat gcctgttttc gccagcgcaa ttgttttatc attattcgtg
    10141 accagataat aaagatcgaa cgacacgcgt gaaaaatcgg caactgccat tgcaatggtc
    10201 aactgctctc catagaacgc ttcacctttg aattggatgg cagcatcaga cataataata
    10261 ccagttcctg caacatccag ctctcggaaa cctagatact gcaaaaaacg taccctagct
    10321 tcatgcaaca gcgaaagcac actatcgttt ccaagatgac cgccgtaatt taaatccgta
    10381 atacgcacat caagcaccgt tgtaaaaagt gttttttcag gaaatgtcaa ttggattctt
    10441 gccataaaaa tgccttcaaa attatatgta tatacctgga atacgtgcca tgggaacaac
    10501 gtcgtaccta gcaaaagcgc ctgcaagaaa tttctgataa ccggtaatgg caatcatagc
    10561 tgcgttgtcg gtgcaatatt cgaacttagg aatgaatact tcccagccca atgccactcc
    10621 catatcggta aggtttttgc gtaaagcggt gtttgcactc acacctcctg atatggcaat
    10681 gcgtgaaatt ccagtttctt ttgcggcacg tttcaccttt ctgagtaaaa tttccacaat
    10741 tccccgttgt acggatgcac agagatcgtt gagattattt tttacaaagt cgggattctt
    10801 tttagtctgt tcttgtaaaa aatacaaaac cgatgtctta aagccgctga atgaaaaatc
    10861 caatccttga attttaggct ctccaaaatc aaaagcttgc gcatttcctt gagcggcata
    10921 cttgtcgatc agtggccctc ccggataagg taatcccagc atctttgcac atttatcaaa
    10981 agcctcgccg gcagcatcgt ctatcgtttg cccaaggatt gccatatcca tggcactgtt
    11041 caccctaacg atctgcgtat gcccgccact taccgtcagg ca
//
LOCUS       DAOQOU010000106        11018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3070_length_11018_cov_5.511356, whole genome shotgun sequence.
ACCESSION   DAOQOU010000106 DAOQOU010000000
VERSION     DAOQOU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11018
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3070_length_11018_cov_5.511356"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLM90_09685"
     CDS             <1..549
                     /locus_tag="PLM90_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HMZ89687.1"
                     /translation="ALVFFQLNEKYTWRPFITKGKKIDSFNGVYVYFNGDVSHVEGRN
                     LSPDGYNFGLKFQCVEFVKRYYYDALHFKMPDTYGNAVDFFNPVVSDGKLNNARGLLQ
                     YSNGSNSKPAIDDILVFDVSSSNPYGHVAIISDVTEQGIEIIQQNPGRFVKSRVNYDL
                     IHRGNKWIIDNKKVLGWLRVPN"
     gene            621..1100
                     /locus_tag="PLM90_09690"
     CDS             621..1100
                     /locus_tag="PLM90_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HMZ89688.1"
                     /translation="MAEQHGFWHKYRSTIVYGISLALLMFLLKWLELRFIIYDHSFEI
                     YVGFIAFIFTGLGIWLALKLSKPKVETIIIEKQVIINADVDFTPNAALISQLDISSRE
                     MDVLNLMAKGHSNAEIARELFISLSTVKTHTGNLFVKLDVKRRTQAIEKAKRLQIIP"
     gene            1207..1755
                     /locus_tag="PLM90_09695"
     CDS             1207..1755
                     /locus_tag="PLM90_09695"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HMZ89689.1"
                     /translation="MKKNILTYGLISGTIVSAIMLFSMNYFSHCEGTVDYTTSMLIGY
                     ASMLLSFSLVFVGVKNYRDKFNGGFITFGKAFQIAILMVLIASTMYVISWVIDYFFFM
                     PDFMDKYAETMIQNLKDSGASAAEVEAKTKEMAVFAERYKNPLFTAMMTYMEILPIGI
                     VVALITSLILRKKPSQPAIDTM"
     gene            1973..3406
                     /locus_tag="PLM90_09700"
     CDS             1973..3406
                     /locus_tag="PLM90_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMZ89690.1"
                     /translation="MKRSSTTMFILLCSISLLAGQPNQQVLDYIEQYKAIAVQEMIEY
                     KIPASITMAQGILESGAGNSELAKESNNHFGIKCHTDWTGDKVYYDDDEKGECFRKYD
                     EVAQSYRDHSIFLSTKPRYASLFELDADDYKGWAKGLKAAGYATNPKYADLLINLIEE
                     YQLQELDKMTMADIKKSNKKDDHKKDDVKKDDVKKDDKNIAKNDEQQDVKEEKTTKKF
                     TWGGYDADVYYFNRIPTVTVKQGETPESLAQKHHVKISLLRDYNDIEDGKPLDVGEKC
                     YLQPKRKKGHDKYHTVVAGETMWTISRDEGVRLAYLYKYNDIEQGQEPAVGEEVYLRF
                     KRHDVMKLATVTNKQEVKSTPEKIIPKPEKEKSAVEQSPVKETYQDTNNDIYIDFEEE
                     KPAPVEAKPEIKTTSTQEINSPKTITAEPVQQDAILVEQYHIVQPKETLYAISKLYGV
                     TVEQIQQWNKLSDNNIRIGQQLIVGYK"
     gene            3403..3930
                     /locus_tag="PLM90_09705"
     CDS             3403..3930
                     /locus_tag="PLM90_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMZ89691.1"
                     /translation="MITLHNLQFEPFIDQAEIKSRIAEMGHSLSNKYIGKEPVFVIVM
                     KGAFMFAADLLQQFHGNCTLHFTKVQSYVGTKAGEITQFSGLPDGLEGKDVVIVEDII
                     DSGNTVAFLLEQLKHVPLNSLFTIALLQKDIPRNTFQSADLIGFIIPDKFVVGYGLDY
                     NELGRNLPAIYQAIT"
     gene            complement(3937..4686)
                     /locus_tag="PLM90_09710"
     CDS             complement(3937..4686)
                     /locus_tag="PLM90_09710"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HMZ89692.1"
                     /translation="MPIFDPRERSGPILPALILANVIPLVGIIYYDVSFFALFYLYWW
                     ETVIISIFRWQKMGWASKPSEPDPNVTYNGKMLTYEQVNNRRGMRIQYFFIRSGMMLF
                     YLLFIVVFVGIMTAVQEDATSFAMALTFTDKWVFWSFIGFVALHLIEYITWIQTEQYK
                     ETTIAELASPFDARVLVMHVVIVGGTFLAMFTSQKLFPDHPHAASIGYGTLFVFLKTI
                     IDVIAWNNNHSRQKAVVKVGNALRGKSNSSK"
     gene            complement(4691..5113)
                     /locus_tag="PLM90_09715"
     CDS             complement(4691..5113)
                     /locus_tag="PLM90_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89693.1"
                     /translation="MKLFNFILGAFLVFITLTFMRCNEVTEITELVKNTEKIQIVFYN
                     GDHQDTYIDITDKSEIKNFGDFVDNKSTPILNCKYDGKIVFFLETNTPSGQKNSTSME
                     FSLSEDCKQVGYMYNQMWQTKNLTNEGYKYLLNLASKQ"
     gene            complement(5140..5430)
                     /locus_tag="PLM90_09720"
     CDS             complement(5140..5430)
                     /locus_tag="PLM90_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HMZ89694.1"
                     /translation="MQAAESIKDILSKNKPYLVSKYSLSSIGVFGAVLQDDFSGGMAV
                     EIIVDFKKPIGIEIIDLGDELETLIHHKVDLVTRNGIEKSYYNLFRKEIQYI"
     gene            5507..6106
                     /locus_tag="PLM90_09725"
     CDS             5507..6106
                     /locus_tag="PLM90_09725"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMZ89695.1"
                     /translation="MINLILFGPPGSGKGTQAEHLKEHFQLLHISTGDLLRNEIANGT
                     KLGMEAKQFMDAGQLVPDEVVIGMLGGAVDQSRSMGKRGIIFDGFPRTTAQALALEHL
                     LADKGTAVSVVLSLVVEEEELTQRILKRGLTSGRSDDNDIDTIRRRVQEYRTKTEPVA
                     GFYKDRDLLVEIEGVGAIEQITQALMNAVNAYSSKHSDG"
     gene            6228..>7060
                     /gene="obgE"
                     /locus_tag="PLM90_09730"
     CDS             6228..>7060
                     /gene="obgE"
                     /locus_tag="PLM90_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMZ89696.1"
                     /translation="MENKNFVDYVKIHCKSGKGGAGSVHFHRDKHTIKGGPDGGDGGR
                     GGHIILRGNAQLWTLLHLKYRKHVIAGDGAYGVGQLRKGADGEDIVLEVPLGTVAKDF
                     ETDEVELEIVEDGQEVIWVPGGRGGLGNNHFKSPTRQTPQFAQPGEPAIEAWKILELK
                     VLADVGLVGFPNAGKSTLLSVVSAAKPEIANYPFTTLVPNLGMVAYRDHRSFIMADIP
                     GIIEGASEGRGLGIRFLKHIERNSLLLFMISCDEKNIADTYKILVNELKKYNKELLTK
                     KR"
     assembly_gap    7061..7160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7161..7375
                     /locus_tag="PLM90_09735"
     CDS             <7161..7375
                     /locus_tag="PLM90_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMZ89697.1"
                     /translation="ILVNELKKYNKELLTKKRILAITKSDLIDDELEAMLKKELPKKV
                     PVIFISSHTQKNIDKLKDMIWKELND"
     gene            7409..8296
                     /locus_tag="PLM90_09740"
     CDS             7409..8296
                     /locus_tag="PLM90_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ89698.1"
                     /translation="MKYSTNNWLLLIGLSVIWGFSFIFMKYGLKTFTWDQVAALRISF
                     SFVFTLPLFIYHFNKIKRAEIKYYCAVGFFGSGLPAFCFTFAQTHIASGITGVLNSLT
                     PVFTFVIGVLLFGMRFERMKFYGLLIALTGAILLVVFDKTEGGESNLLYALPLFLATM
                     SYAMSANIVKKYLQTAHPLALGAVGFMFIGIPAMSYLFSTDFVDRASMETFAISFSSV
                     MALSFFGTVLASLGYYMLIQKTDPIFGSLVAYLIPIVAIIAGAADGETLKAYHLAGMA
                     FILLGIWVINRRSASKATA"
     gene            8384..10540
                     /locus_tag="PLM90_09745"
     CDS             8384..10540
                     /locus_tag="PLM90_09745"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89699.1"
                     /translation="MKINLHISIASCTKRIFLVGFCSLFSIIPANAQAPEIEWQNTIG
                     GSLADNLYSVSQTFDGGYILGGNSYSSISGDKTENSHGSSDYWIIKLNATGEIEWQKT
                     IGGAGGDFLKCVAQTLEGGYIVGGYSNSNISGDKTENCLGYDDYWIIKLNTDGEIEWQ
                     NTIGASNDDDFRTLIQTDDGGYFVGGVSFSNVNGDKTEHTNGFMDYWVLKLNSLGSVE
                     WQNTIGAAGTDVLESVTQSADGGYLVGGYSFSGASGDKSENNVGGNDLWLVKLNSLGE
                     IEWENTIGGNLDDKLQSVNVTSDSGYIIGAQSTSTISGDKTEDSIGYSDYWIIKLNSF
                     GIIEWQNTIGGTESENIGSVFQTSDGGYFIGGYSKSDISGDKTEIGYGSDDFWLAKTN
                     NVGMIEWDFNIGGNSSDILRCAIQTADGGFIVGGYSSSPISGNKTENSFGITNDYWVV
                     KLAGPACTPAPEMCNLIDDDCDGLIDEGASPAISISAGGATTFCQGSNVILTATHTGT
                     GLQWKKNGTTIPGATGVTYAATKTGIYTCESSNACGATLSASISVTANKNPPASITAG
                     GPTSFCAGGSVVLTANAGGGLSYQWYKGATPIAGATNIAYTASTAGNYKCRVTKTATG
                     CFKNSNAIMVSIVCRGGENETPSQILYAYPNPATTQLNITCSANINTAQLYNGLGQLI
                     MEISVSDGYAEADISTLTAGMYLIRAGGEVAQWVKQ"
     gene            complement(10846..11010)
                     /locus_tag="PLM90_09750"
     CDS             complement(10846..11010)
                     /locus_tag="PLM90_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HMZ89700.1"
                     /translation="MLKCTIFLRDMNDFAAVNAVYGEYFQEYPPARETVAVVGLPKNA
                     RVEISAMVSR"
BASE COUNT         3289 a         2177 c         2379 g         3073 t
ORIGIN      
        1 gcacttgtgt tttttcaatt aaacgaaaaa tatacatggc gaccatttat tactaaaggc
       61 aaaaagattg atagcttcaa tggcgtttat gtttatttta atggagatgt gtcacatgtt
      121 gaaggaagaa atcttagccc tgatggctat aattttgggt tgaaatttca atgtgtcgag
      181 tttgttaaac gatattatta cgatgcacta cattttaaaa tgcccgacac ttatggcaat
      241 gcggttgatt ttttcaaccc tgtcgtgagc gacggaaaac tgaataacgc ccgtggatta
      301 ttgcaatatt ccaatggcag caacagtaaa ccggccatag atgatattct tgttttcgac
      361 gtgtcttcat caaatcctta tggtcacgtc gctattatat cggatgttac tgaacagggc
      421 atcgagatca tacagcaaaa cccgggcagg tttgtcaaat cacgggtgaa ttatgacttg
      481 attcatcgag gtaataagtg gattatcgac aataaaaaag tgctgggttg gctgcgagtt
      541 cctaactgaa tgccttattc ggtcgatccg cttacttccg cttgatcgcc actcttctcc
      601 aaatttcaga tatttgcaat atggcagagc agcatggttt ttggcacaag taccgttcta
      661 ccattgtata tggtatcagc ctggctttgc tcatgttcct gcttaagtgg ctcgagctgc
      721 gtttcataat ttatgatcac agctttgaaa tttatgtcgg atttattgcc ttcattttca
      781 cgggattggg tatctggctg gcactcaaac tgtcgaaacc caaggtggaa accattatta
      841 tagaaaagca ggtgattatt aatgccgatg tcgattttac acccaatgct gcacttatca
      901 gccaattgga tatcagctca agagaaatgg atgtgctcaa tctgatggct aagggacaca
      961 gcaatgccga gattgcacgc gaattgttta tttccctcag cacagttaag acacataccg
     1021 gaaacttgtt tgtcaaactg gatgtcaagc gccgaacaca ggctattgag aaggcaaaac
     1081 gacttcaaat catcccataa ttaccatact ttagtaggaa tcggcactat ttcaggcaaa
     1141 aatggccgaa agtatgaccg gaaaaatacc ctgcaacgcc acatttgcgg cataaaataa
     1201 actcaaatga aaaagaacat ccttacctac ggcctcatct caggaaccat cgtttctgca
     1261 atcatgctgt tttctatgaa ttatttcagt cactgtgaag gcactgtaga ctataccacc
     1321 agcatgctca tcggctatgc ctcgatgttg ctttcattct cgttggtatt tgtaggtgta
     1381 aagaactatc gcgataaatt caacggcgga ttcatcacct tcggtaaagc cttccaaatt
     1441 gctattctca tggtgcttat tgcctctacc atgtatgtga tttcatgggt gattgattac
     1501 ttcttcttca tgcccgactt tatggataaa tacgccgaaa cgatgattca aaatctcaaa
     1561 gatagtggcg ccagtgctgc agaggtcgag gccaaaacaa aagaaatggc cgtatttgcg
     1621 gagcgatata aaaacccatt gttcactgct atgatgacct atatggagat actgcctatc
     1681 ggaattgttg ttgctttaat cacttctctg atactccgta aaaaaccgag tcagcctgca
     1741 atagacacca tgtaatcttg tttttatcgg ctaaaagcgc gaggtatgca tgcagtgtcg
     1801 gcactgttta tgcatttctt cgcgcctttt ttatgctcat tgtatgttct caaaattgga
     1861 ttctgccctt cattttagtg gaaaacacat gcaaggcacg gttttggggt gagataattt
     1921 tacattggtg cattacgttt ggtaaaggcg cctttgaaaa cctatgtcga gtatgaaaag
     1981 aagcagcaca accatgttca ttctgctctg ctccatcagc ctgttggccg gtcagcccaa
     2041 tcaacaggtg ttggactata tagagcagta caaggccatt gccgtgcagg aaatgattga
     2101 gtataaaatt cctgccagta tcaccatggc tcaaggtatc ctggaaagcg gcgcaggaaa
     2161 tagtgaactc gcaaaagaat caaataacca ttttggtatt aaatgtcata ccgactggac
     2221 cggcgacaaa gtgtattacg atgacgatga aaaaggcgag tgctttcgca aatatgatga
     2281 agtggcgcaa tcgtaccgcg atcatagtat ttttctcagc accaaaccgc gatatgcttc
     2341 cttgtttgaa ttagatgctg atgattataa gggttgggcg aaaggattga aagctgccgg
     2401 ttatgccact aatcctaaat atgccgattt gctcattaac ctcattgagg aatatcaatt
     2461 gcaggagttg gataaaatga caatggctga tatcaaaaaa tcaaacaaaa aggatgatca
     2521 caaaaaggac gatgttaaaa aagatgatgt aaagaaagat gataaaaaca ttgcaaagaa
     2581 tgatgagcaa caggacgtga aggaggagaa aacaaccaag aagtttacct ggggcggata
     2641 tgatgctgat gtatattatt ttaatcgcat accaaccgtt accgttaaac aaggtgaaac
     2701 accggaatca ctcgcccaaa aacatcatgt aaaaatcagc ctgctgcgcg attataacga
     2761 tatcgaagat ggtaaaccac tcgacgttgg cgagaagtgc tatctgcaac caaaacgaaa
     2821 aaaaggtcat gataaatatc ataccgttgt agcaggagaa accatgtgga cgatttcccg
     2881 cgatgaaggt gtgcgattgg catatttata taaatataat gatatcgagc agggacaaga
     2941 acctgcagtt ggcgaggaag tatatctgcg atttaaacga catgatgtca tgaagttggc
     3001 aacggttaca aataagcagg aagtgaaatc aacacccgaa aaaataattc ctaaaccgga
     3061 aaaagaaaag tctgctgttg aacaatcgcc tgtaaaagaa acctatcaag acaccaacaa
     3121 tgatatttat atcgattttg aagaggaaaa accggcgccg gtggaagcaa aaccggaaat
     3181 aaaaaccacc tcaacgcaag aaatcaattc gccaaaaaca ataactgctg aacctgttca
     3241 acaagatgcc attttagttg agcaatatca tatcgtgcaa ccaaaagaaa cactttatgc
     3301 tatttccaaa ttatatggcg taacggtaga acagattcag caatggaata aattgagtga
     3361 taataatatc cgcatcggtc aacaattaat tgtcggatat aaatgataac cctccacaac
     3421 ctgcaattcg aacccttcat tgatcaggcg gaaataaaat cgcggattgc ggaaatgggg
     3481 cattcccttt caaataaata cattggaaaa gaacctgtat ttgtaattgt aatgaagggt
     3541 gcttttatgt ttgccgccga tttattgcaa caatttcatg gaaactgtac cttgcatttc
     3601 accaaagtgc aatcttatgt cggcactaaa gccggagaaa taacacaatt ctcaggtctt
     3661 ccggatgggc tggaaggaaa agatgtagtg attgttgaag acataatcga ctccggaaat
     3721 accgttgcat ttttattaga acagcttaag catgttcctc tcaattcctt atttaccatt
     3781 gcacttttgc agaaagatat tccgcgcaac acatttcaat ccgccgacct tatcggattt
     3841 atcattcccg ataaatttgt ggtaggctat ggattagatt ataacgagtt gggtagaaat
     3901 ttgcctgcaa tttatcaggc aataacctaa gcaatgttac tttgaggaat ttgattttcc
     3961 tcgcaaggcg ttgcctactt tcacaacagc tttttgtcgt gaatgattgt tgttccaggc
     4021 aataacatcg atgatcgttt tcaaaaaaac gaacaaagtg ccataaccaa tactcgctgc
     4081 atgcggatga tcgggaaata atttctgact tgtaaacatg gccaggaatg ttccgccaac
     4141 aattaccaca tgcataacta aaaccctggc atcaaaaggt gaagctaatt ctgctatagt
     4201 tgtttctttg tattgttcgg tttgtatcca ggtgatgtat tctatcaggt gtaatgcaac
     4261 aaacccaatg aaactccaga acacccattt gtctgtaaag gtcagcgcca ttgcaaatga
     4321 agttgcatct tcctgaacgg cagtcataat gccgacaaac acaacaataa acagtaaata
     4381 aaataacatc attccggagc gaatgaagaa atattgtatc cgcattcctc ttcgattatt
     4441 cacttgctca taggtgagca ttttaccatt gtaggtaaca ttaggatcgg gctcggaagg
     4501 tttggaagcc catcccattt tctgccagcg aaatatgctg atgattaccg tttcccacca
     4561 atacaaataa aataaagcga aaaaacttac atcataatag attatcccaa ccaaaggaat
     4621 aacattggca agtatcaatg ccggaagtat cggaccgctg cgctctctgg gatcaaatat
     4681 gggcatgatg ctattgtttt gatgccagat ttagaagata cttatagccc tcgttggtca
     4741 gattctttgt ctgccacatt tgattgtaca tataaccaac ctgtttgcaa tcttccgata
     4801 aactgaattc catactggtg gagtttttct gtccggaagg tgtatttgtt tccaggaaga
     4861 aaacaatctt accatcgtac ttgcaattca gaattggcgt tgatttatta tcaacaaaat
     4921 cgccgaaatt ttttatttct gacttatcag tgatatctat ataagtatcc tgatgatcgc
     4981 cattgtagaa tacgatttga atcttttcag tgtttttcac taattccgta atttcggtca
     5041 cttcattgca acgcatgaat gtgagtgtaa taaacaccaa aaatgcacct aaaatgaaat
     5101 tgaagagttt catcagtgat taaaaataca tcatcttgtt cagatgtatt gtatttcctt
     5161 tctgaacaag ttgtaatacg acttttcaat gccgttacgc gtaactaaat ccaccttatg
     5221 atgaattaat gtttccagtt catcgcccag gtcaataatc tctataccaa taggtttctt
     5281 gaaatcgacg atgatttcaa cggccatacc acctgaaaaa tcatcttgta acacagctcc
     5341 aaacacgcca atgctgctca aactgtattt tgataccaga tatggtttat tttttgaaag
     5401 gatgtctttt atcgattcag ctgcctgcat agctcaaagg taatcattcc gccacacgga
     5461 attacctata acaaccgtat ctttgccgcc taagttcaca ccgactatga tcaatctcat
     5521 cttattcggc cctccgggaa gcggtaaagg cacacaggca gagcatttga aggaacattt
     5581 tcaactcctc catatttcta ccggcgacct gctgcgcaat gagattgcca atggcactaa
     5641 gctgggtatg gaagccaaac aatttatgga tgccggccaa ttagttccgg acgaagtggt
     5701 gataggcatg cttggcggcg cagttgacca atccaggagc atgggaaaac gaggcatcat
     5761 tttcgacgga tttccccgca caaccgcaca ggcactggct ttggagcatt tgcttgccga
     5821 caaaggaacg gcagttagtg tggtgcttag cctggtggtt gaggaagagg aacttacgca
     5881 acgcatcctc aaacgtggac ttaccagtgg tcgctccgat gataatgaca tcgataccat
     5941 acgccgtcgc gtgcaggaat atcgcacaaa aacagagccg gttgcaggtt tctataagga
     6001 tcgcgatttg cttgttgaaa tcgaaggcgt aggcgctatc gaacaaatca cgcaagcatt
     6061 gatgaatgcc gttaatgctt attcatccaa acattccgac ggttaatgta tccgaaagca
     6121 gatttgctga tggctgctaa tgcatagccg aagggtgaat ctagcaggat ataccacatt
     6181 acttttcgcc caaagccttt taggtcaagg gttttgcaat aaattacatg gaaaataaaa
     6241 actttgtcga ttacgtaaag atccactgca aaagcgggaa aggcggcgcc ggttcagtgc
     6301 acttccaccg cgataagcac acgattaagg gtggtcccga tggcggcgat ggcggtagag
     6361 gcggacatat cattttacgc ggcaatgccc aattgtggac cctattgcac ctgaaatacc
     6421 gcaaacacgt gattgccggc gacggggctt acggcgttgg acagttgcgc aagggtgccg
     6481 atggagagga tattgtgctg gaagtaccac ttggaacagt agccaaagat tttgaaacgg
     6541 atgaagttga gctggagatt gtagaagatg gtcaggaggt gatttgggta cccggaggca
     6601 gaggcggact cggcaacaac cattttaagt cgccaacacg acaaacccct caatttgcac
     6661 aaccgggaga acctgccata gaagcatgga aaatccttga gctgaaagtg cttgccgatg
     6721 tgggtttggt aggatttccc aatgccggaa aatctacctt gctgagcgtt gtcagtgccg
     6781 caaaacctga aatcgccaat tatccattta caaccctcgt gccaaatctc ggaatggtag
     6841 cctaccggga tcacagaagc ttcatcatgg ccgatattcc cggaatcata gaaggtgcca
     6901 gtgagggtag gggattggga attcgtttcc tcaaacatat agaacgcaat tcgctgctgc
     6961 tgtttatgat cagttgcgat gaaaaaaata ttgcggatac ttataagatt cttgtcaacg
     7021 aactgaaaaa atataacaag gaactactca ccaaaaaacg nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn agattcttgt caacgaactg aaaaaatata acaaggaact
     7201 actcaccaaa aaacgcatcc tcgccattac caaaagcgat ttaattgatg atgaactgga
     7261 agcaatgttg aaaaaagaat tgcccaaaaa agtacctgtg attttcattt catcacatac
     7321 ccaaaaaaat atcgacaaac tgaaagatat gatttggaag gaacttaatg attgagttgt
     7381 ggtttgttga caagaatatt aaactctcat gaaatattct actaataatt ggttgctgct
     7441 gatcggactt agtgtgatct ggggcttttc attcatattt atgaaatatg gattgaaaac
     7501 attcacatgg gaccaggtag cggcgctgcg tatttctttc tcatttgtgt tcactcttcc
     7561 gctgtttatt tatcacttca ataaaattaa gcgtgcagaa ataaagtatt actgtgctgt
     7621 aggattcttt ggcagcgggc ttcctgcttt ctgttttact ttcgctcaaa cacatatcgc
     7681 cagtggtatc acgggtgtac tcaattcgct gacacctgta tttacatttg tgattggcgt
     7741 gttgttattc ggcatgcgtt tcgagcgaat gaaattttat ggtttgttga tagcgcttac
     7801 aggagcgata ttattggtgg tgtttgataa aacagaaggt ggcgaaagta atttgttgta
     7861 tgccttgcca ttatttctcg cgacaatgag ttatgcgatg agcgcgaata ttgtaaaaaa
     7921 atatttgcaa acagcgcatc cgctcgcatt gggtgctgta gggtttatgt tcatcggcat
     7981 tcctgcaatg agttatttgt tcagcactga ttttgtggac cgcgcatcga tggaaacatt
     8041 tgctattagc ttttccagtg tgatggcatt gtcatttttt ggaaccgtgc ttgcgtcatt
     8101 aggatattac atgttgatcc aaaaaacgga cccgatcttt ggcagtctgg ttgcctattt
     8161 aattcccatc gtagccatca tcgccggcgc agcggatggc gaaacgctca aagcctatca
     8221 tcttgcaggc atggcgttta ttttacttgg aatttgggtg attaatcgaa ggagtgcttc
     8281 gaaagcaacg gcttaactca ttgaccctct ttggtttaat tcaaaggatg gtatgagata
     8341 attcgagtat tcatcttttt agtattttta taaaaaatag ccaatgaaaa ttaatttaca
     8401 tatttctatt gcgtcgtgta ctaagcggat ttttcttgtt gggttttgtt cgttgttctc
     8461 aataataccc gcgaatgctc aggcgcctga aattgaatgg cagaacacga ttggcggaag
     8521 tttagcggat aacctttatt ctgtttcaca gacatttgac ggtggatata ttttaggcgg
     8581 aaattcttat tcaagtatat ccggggataa aacagagaat agccatggga gctcggatta
     8641 ctggataatt aaactcaatg caacaggaga aattgagtgg caaaagacaa ttggtggtgc
     8701 cggtggtgat tttctaaaat gtgttgctca aactcttgaa ggcggatata ttgttggagg
     8761 atattctaac tcaaatatta gtggcgataa aacagaaaat tgccttggct atgacgacta
     8821 ttggataatt aaattaaata ctgatggtga gattgaatgg cagaacacaa ttggagccag
     8881 caatgatgat gactttagaa cacttattca aacggatgat ggtggatatt ttgtaggtgg
     8941 ggtttcattt tccaatgtaa acggtgataa aacagaacat accaacggtt ttatggatta
     9001 ttgggtacta aagctcaaca gtctcggttc tgttgagtgg cagaatacga ttggagcggc
     9061 tggtacagat gtgcttgaat cagttaccca atctgcagat ggaggttatt tagttggcgg
     9121 atattcgttt tccggagcct caggcgataa gagcgaaaat aacgttggtg gcaacgacct
     9181 atggcttgtt aaattaaatt cgctgggaga aattgaatgg gagaatacga ttgggggtaa
     9241 cctagatgac aaacttcaaa gtgtgaatgt tacatcggac agtgggtaca ttattggagc
     9301 gcaatccact tcaactattt ctggagataa gaccgaagat agcatcgggt attctgatta
     9361 ttggattatt aagcttaatt catttggaat tattgaatgg caaaatacaa ttggtgggac
     9421 ggaatctgaa aacattggat cagtttttca gacgagcgat ggcggttatt ttattggcgg
     9481 ctactcaaaa tctgatattt ctggtgataa aacagaaatt ggatatggat cagatgactt
     9541 ttggttggca aaaactaata atgttggaat gatagaatgg gattttaata ttggtggtaa
     9601 ctccagtgat atcctccgat gcgccattca aacagcggac ggagggttta ttgttggagg
     9661 ctattcaagc tcaccaattt ctggtaataa aactgaaaat agttttggta taactaatga
     9721 ttattgggtt gtaaaactag ccggccctgc atgtacacct gctccggaaa tgtgtaacct
     9781 aattgatgac gactgcgacg gtctcattga cgaaggtgca tctccagcaa tttctatttc
     9841 agccggaggt gcaacaactt tctgccaagg tagtaatgtc attcttactg caacgcacac
     9901 cggtaccgga ttgcaatgga agaaaaatgg aacaacaatt cccggtgcaa ccggagttac
     9961 atatgctgca acaaaaacag gtatttatac ttgtgaatca agtaatgctt gcggagcaac
    10021 tttatctgca tcgatcagtg taacagcaaa taaaaatccc cctgcaagta taacagccgg
    10081 cggcccaacc agcttttgcg caggcggcag tgttgtattg acagctaatg caggcggagg
    10141 attgagttat caatggtata agggggcaac accgatcgca ggtgccacta atattgcata
    10201 tactgcttca acagcaggta attataaatg tagggtaaca aagacagcta caggctgctt
    10261 caagaacagc aatgcgatca tggtgagtat tgtatgcagg ggtggagaaa atgaaactcc
    10321 ttctcaaatc ctttatgctt atcccaaccc tgcaacaact cagttaaata tcacctgctc
    10381 tgcaaatatt aataccgcac aactctataa cgggctgggt cagctgatca tggaaatttc
    10441 tgtatcagac ggatatgcag aagcagacat tagcacattg acggcaggta tgtatctcat
    10501 ccgcgcaggc ggtgaagtgg cgcagtgggt gaagcaataa tttccggaat ttgatttggc
    10561 ctattcgcgt ttgattttgc tgatacttct tactaattga aggttgagac ctggaagttg
    10621 cggttgctct tcactcattg gggttcaatc gcggagtttg cggagttagc ggagtttcat
    10681 agatatgtct cagcccaccc attagccggg tttcaatcgc ggagttagcg gagtttcgca
    10741 gatgtgtctc catctacctt cggcaatgca gagtatgttc aaaacacaca acacgaattc
    10801 actactcatc cactcatcca ctcacccact cacccactga ccccctcacc tactcaccat
    10861 cgcacttatc tccaccctcg cattcttcgg taacccaaca accgcaaccg tttcccttgc
    10921 aggtgggtat tcctgaaaat attcgccata aacagcattt actgcggcaa aatcattcat
    10981 atcgcggaga aaaatggtgc atttcagcac attaccca
//
LOCUS       DAOQOU010000107        10963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3097_length_10963_cov_6.272552, whole genome shotgun sequence.
ACCESSION   DAOQOU010000107 DAOQOU010000000
VERSION     DAOQOU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3097_length_10963_cov_6.272552"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..2086)
                     /locus_tag="PLM90_09755"
     CDS             complement(56..2086)
                     /locus_tag="PLM90_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ89701.1"
                     /translation="MKIRILVLLLLAVATNVLSAQESLLWKVTSPNGKFTSYILCSTE
                     LPGVENYDIITPATPIISKVNTVAFFNVPDAAEIQNIPTFMKAPGDNTLKGYYKREDR
                     IRFELMVADKLKENIETYYAYKPLYTLQLMREKDHAAGLDYQQTILLDIALQQKKPTL
                     SLMTIRQIAGVMDLMDFNTQATVLSNYINTASLYIDADNEKFQAYAGQSMKDYLRVVN
                     SSEQDAYITTMIGSMNDMLMKKMDPLLQQQSALFILDADLIGGDLGIIKKLQAKGYTV
                     VAEPFDYKKYADGMKPDPNLTNNTINNATASNIPADDFPSFAIQPVTGGYVDPETIKV
                     INIKDNPGKKPGYLAYRDPFGDFFDMQSADTLFMESWYELRGTDANFHVKVPVKGDWE
                     ESSTPWLEGGEIKKFVYQTNHAKSDVFYSIGYTVYPPSFKADDRDAFFNQFIQSTQDQ
                     ISGQIISQRIVSNPNFTGREFTAVVGDSFFVRSQFLLQDNVLYQLLVGGPGDNSYSVY
                     AEAFLNSFQTASNVLVNWFFFEQPSFSCYLPMEPSKSTKTYTLPSGPLQVQTFSAPDL
                     KEAIEYQVVVSTYPAGHKFSNKKTFFDELIANAEKQYIGKALKVESVENNGVEGRYVE
                     MQLMNRKIYRMYIYFDGTSTYQFVAGGDSNVMVSNNVNRFIQSMKMSAAKKK"
     gene            2168..2686
                     /locus_tag="PLM90_09760"
     CDS             2168..2686
                     /locus_tag="PLM90_09760"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HMZ89702.1"
                     /translation="MDIFEISGEGAIEILIRVFIVYLVVVLGMRLVGKREIAQLSVVD
                     FVMMLLISNAVQNAMLGDNTSLSGGIIAALALFLINFVLRTLEYRNKRLKGLLEGEPV
                     TLVYQGRLLEGNLKKARITEAELMSAIREHGVNEIGQVDLGVLEINGSISILSNDFSK
                     RSSTEKNEDVKK"
     gene            2673..3611
                     /locus_tag="PLM90_09765"
     CDS             2673..3611
                     /locus_tag="PLM90_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89703.1"
                     /translation="MSRSNNRNMQHLSFAKNTITGIIFFACVQFTNAQCVVGDCINGN
                     GTYKLENGDMYTGNWINGVREGYGRYDWASGAYYVGNFKNNMLDGEGAFYGVDGTKMI
                     GIFIKNNYAGTAADTANRKIYSPDDEIQKAYNDIKKRDSLAKATTLAKAERVEFCSLL
                     RRLTTDFPNQFSSFLGERQEIVLSNSKNWYATIMATGSLEAGVNSFSGKPERSYYNIL
                     FESVDSVSALAQYAQYVEQVKTCNNGCCTMVYDESTFHAKSQSGYSTIWATLAVKEGL
                     EPLLYNNLIIEIECLTKMAEPGWIVVLRMYDAQTLK"
     gene            3732..4652
                     /locus_tag="PLM90_09770"
     CDS             3732..4652
                     /locus_tag="PLM90_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89704.1"
                     /translation="MKQFFAAITLVFFVQLADAQGFVFDSVGVQHMTIGKVMLKSDTW
                     VIRVERGGGYDDFWPVNLPEKYAIAGQEVVFEGALGRIPMQARLVGTPVKLSMIRVLY
                     RTTPRDDDKSGESIAKEKPINPDSVGYIEKQTGRILMVNDMYLIETTTVDAKGNEEVK
                     RYVPDFLPDDFKMAGQQITFSAIILKHDPNVRMMGTPVKIKELMMEENTPFDATQLQE
                     SVAPYYPFDSAGRLVETKAMVKLIADVYILEVTIDNQNNITRYIPAILPESFKKEGTI
                     VMISGTIGKIPPNVRMMGTPLTLESIRLVE"
     gene            complement(4879..5649)
                     /locus_tag="PLM90_09775"
     CDS             complement(4879..5649)
                     /locus_tag="PLM90_09775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ89705.1"
                     /translation="MKQIIFAICCVALLSNCSEKKSETTEDIVPQKTDTVISTTTPEQ
                     IQSQTTTTDTMDDNKVYYVNISTTYGDMLVKLYNETPAHRDNFIKLVKEGYYNDLLFH
                     RVIKDFMIQGGDPDSRNAGPNVQLGAGGPNYKIPAEIKPGLYHKKGALAAARQGDQVN
                     PSRASSGSQFYIVQGKRSTEAELNNISRGYGVNFSPEQKKVYETIGGTPFLDGSYTVF
                     GEVVKGLEVIDKIAAVKTMYGDRPAEDVKMKITIAEGK"
     gene            complement(5646..6008)
                     /locus_tag="PLM90_09780"
     CDS             complement(5646..6008)
                     /locus_tag="PLM90_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89706.1"
                     /translation="MSLTHEEIPIDFDPRWIAVLQRLRDQFGKKPELETILFLIGMNE
                     LGKVEDKFSKEQKQDLMHIAVCRLLSADGYYRLAGWDDDQWPMWEATKELPAWSAAEQ
                     EIIIKQHIIRYFEENKLL"
     gene            complement(6114..6470)
                     /locus_tag="PLM90_09785"
     CDS             complement(6114..6470)
                     /locus_tag="PLM90_09785"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HMZ89707.1"
                     /translation="MFTAPFSFEGRINRAEYGKSFGLYIVVAIIMNAIIANMLSQGAL
                     FLLLLYIPLVWFFLAQSVKRAHDLGNSGWYVLIPFYTIYLLFPLGQHGSNKYGPNPDG
                     IGEDTYYSDNAPIDKV"
     gene            complement(6719..7441)
                     /locus_tag="PLM90_09790"
     CDS             complement(6719..7441)
                     /locus_tag="PLM90_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89708.1"
                     /translation="MRTFIVFILLVFVCNVLRAQSNAKFNMGVGYGLGTHITNDGLNF
                     VIGRYNDTRPYLSKPMDEVHFFRGWNVAVETYFPKFLMEFEFIGHTSNVSAESSSTSD
                     ARDFKYRMNTFNMGYAYQLTGQKDKVQGLYGGFDISTISVKNFTRTYTIGNDPGDFYE
                     INWDLIIGVSPYLQYAGGRFTFKGYYQWMIFKQNFWDVNVALNPATWSGDAYESNEGK
                     ANTIGMVLRVNLWENDRPKSYY"
     gene            complement(7504..8337)
                     /locus_tag="PLM90_09795"
     CDS             complement(7504..8337)
                     /locus_tag="PLM90_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89709.1"
                     /translation="MAFLFSGFRSAAQTTATTLRIDTLPKGTFVIIADVNEKDPHFKK
                     KKNFIGDTIYLLSEAIIDTKGWYAGTFNLDGAYEDYFYFSFLPVAGQQYEPQKQVAAP
                     ISEEFRQGPLSFDASAVNKFINELYQAYRDSFKLVTGTYVSFSEGYTTTLQFPGAVEN
                     NLSAIDPSLGYYAYLGDYANEQDGYAAYTALLEAIRQVKVYPDIYSDAYYFPSMGAAK
                     AFASGETLYSNVGFLMQDIGAVINVQGDESVIIEVTLMTNFKDGYFLTFDFYNKILAG
                     F"
     gene            8467..9564
                     /locus_tag="PLM90_09800"
     CDS             8467..9564
                     /locus_tag="PLM90_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89710.1"
                     /translation="MKNLQHIPNITPQLNAVRARLQHTQDLIQARAAAHVLHPNEYPL
                     PADGNSLEHHFVEVYQLLSDKGKKNFEENAKLIMGYMTADRRAELGDLANLNHKSPLS
                     TVEQVKQLGTNDTMRIKQEHVTANETLLRKHLPKSFPGIAFEGPLREVDVRGQNPRTL
                     SFFVDSIHCEKTTDLRKDEISLFGFTFDAFGQGTKVEQFEVGKFKKGETIALGSKAKL
                     RDFDLRAPGFFPQSFFTNLFLFERDWVNFEKTGKRLMMLGAALSATFLTVGFYLLTLS
                     PIPVLGTALGIASIACLLMTAISYGFALLIGAMCEDVSGGAGTAFTFNIPPVIPVGTA
                     FTETLDFTIEGGLFGVHKAKYSAQVRWERTA"
     gene            complement(9574..10911)
                     /locus_tag="PLM90_09805"
     CDS             complement(9574..10911)
                     /locus_tag="PLM90_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ89711.1"
                     /translation="MKALPFLLLVTGTILTNVIFAQMTTVSSDKEWEKQEVFLKNTME
                     AEYIIRLGDVDNLGFGWPEGFDPFCGRMTDSHAYPWEPQANDLPGFDRILMSSKWHGM
                     QDFPCGAEGYSFADPSMQYKPVVWNLPMNEMKDATVQNAWLQLFIDDFQAPSFCSKYI
                     ILVNGKHFLEAEKMLNAIDQTGPVGKLISIPFPEDWYPLFANGGNCMISIDEVNGVGD
                     GFALDFVRLLVNRKRENSCKGNVRGVVMIKDTENPIANATVFTSNQSFAKTNANGEFI
                     IADIPTGFDVVSASAEGYIDGSATADIGQGDDNDPIIIYLEKGKSAKFDNNNITVGQS
                     ITLNNILFDQGKADLRPESKTELEKIVAFMQTNPSAEIELSGHTSSEGDYAMNKSLSY
                     KRVKACKDYIVSKGISESRIMAVGFGPDKPVAPNDTESNRAKNRRVEMRVTKL"
BASE COUNT         3195 a         2240 c         2328 g         3200 t
ORIGIN      
        1 tacttgatac ctgatacttg atacctgata cctgatactt gatacctgat accaactatt
       61 tcttcttcgc ggcagacatt ttcatcgatt ggataaatcg attaacgtta ttgcttacca
      121 tcacattcga atcgccgccg gcaacaaatt gataggttga agtgccatca aaataaatat
      181 acatgcgata tattttgcga ttcattaatt gcatttcaac atagcgccct tcaacaccgt
      241 tattttcaac ggattccact tttaacgcct tgccaatata ttgtttctcg gcattggcaa
      301 tcaattcatc gaagaatgtt tttttattgc tgaatttatg ccctgcggga tacgtactta
      361 ctactacttg atattcaatt gcttccttta aatcaggagc gctgaaagtt tgaacctgaa
      421 gtggtccgct tggaagtgta taagtctttg tcgattttga aggctccatc ggcaaataac
      481 aactgaatga aggctgttca aaaaagaacc agttcactaa cacattgctt gctgtttgga
      541 atgaatttag aaatgcttct gcatatacag aataagaatt atcgccggga cctccaacca
      601 ataattgata taatacatta tcctgcaata aaaattgcga gcgcacaaaa aatgaatcac
      661 caacaactgc agtgaattcc cttcccgtaa aattcggatt ggaaacgatg cgttgcgaaa
      721 ttatctgacc gcttatctga tcctgtgtgc tttgaataaa ttgattaaag aatgcatcgc
      781 gatcatctgc tttaaatgac ggtggatata ccgtatatcc aatagaataa aatacatcgc
      841 tttttgcgtg gttggtctga taaacaaatt tctttatctc accaccttca agccatggag
      901 tagaagattc ttcccagtcg cctttcacag gaacttttac atgaaagttc gcatcggttc
      961 cgcgcaattc ataccagctc tccataaata aagtatccgc cgattgcata tcaaaaaaat
     1021 cgccgaaggg atcgcgataa gcaaggtaac cgggtttttt tccgggattg tccttgatat
     1081 tgataacttt aattgtttca ggatcgacat acccgccggt caccggttga atggcaaatg
     1141 acgggaaatc atcggcagga atatttgaag cggtggcatt gttgatggta ttattggtaa
     1201 gattcggatc cggtttcata ccatcggcat attttttata atcaaaaggt tctgcaacaa
     1261 cagtgtaacc tttagcctgt aattttttga taattccaag atcgccgcca atgagatcgg
     1321 catcgagaat gaataacgcc gattgctgtt gcagcagtgg atccattttc ttcatcaaca
     1381 tgtcgttcat gctgccaatc attgtggtga tataggcatc ttgctccgaa gagtttacta
     1441 cgcggaggta gtctttcatc gattgcccgg cataggcctg aaatttttca ttgtcggcgt
     1501 ctatatacaa gctggcagta ttgatatagt tgcttaatac ggttgcctgt gtattgaaat
     1561 ccatcagatc cataacacct gcaatctggc gtatggtcat gagcgataaa gtaggtttct
     1621 tctgttgcag ggctatgtcg agcaggatgg tttgctgata atcaagaccg gctgcatgat
     1681 ccttctcgcg catcaattgc agggtgtaca gtggtttgta ggcgtaataa gtttcgatat
     1741 tctctttcag tttatcggca accattagct caaagcgaat cctgtcctca cgtttgtaat
     1801 acccttttag cgtattgtcg ccgggagcct tcatgaacgt aggaatattc tgaatctcag
     1861 ccgcatcggg cacattaaag aatgctacgg tatttacctt gctgatgata ggtgttgcag
     1921 gagtaatgat atcgtagttc tcgacgcccg gaagctcggt agaacaaagg atgtacgatg
     1981 taaacttacc attagggctt gtaaccttcc agagcaggga ctcctgcgcg gaaaggacat
     2041 tagtggccac agccaacagt aacagaacca ggatgcgaat cttcatgcta ttgcttgatt
     2101 gggtaaattt aaggcaaaaa ttgcggtacc tgctataaat ggattacgta ctgcagcatt
     2161 tcatgacatg gatatttttg aaattagtgg cgaaggagcc atagaaatcc tgattcgggt
     2221 atttattgtt tacctcgtgg tggtgcttgg catgcgtctt gtaggcaaac gggagattgc
     2281 gcagctgagt gtggttgact ttgtgatgat gctgttgatc agcaatgccg tgcagaatgc
     2341 catgttgggg gataacacct ctctctccgg cggaatcatt gctgccctgg ccttgttttt
     2401 gatcaatttc gtgctgcgga cactcgaata tcgcaacaaa cgcctcaaag ggttattgga
     2461 aggcgaaccc gtaacattgg tgtatcaggg gcgattgctg gaaggaaatc ttaaaaaagc
     2521 acgtataacg gaggccgaat taatgtcggc tatacgtgaa cacggggtga atgagatcgg
     2581 acaggttgac cttggtgtgc tggaaataaa cggaagcatc agtatcttgt cgaatgattt
     2641 ttcgaagcgc agcagtactg aaaaaaatga agatgtcaag aagtaataac agaaatatgc
     2701 aacacctttc ttttgctaaa aataccatca ccggcatcat tttttttgca tgtgtacaat
     2761 ttacaaatgc gcaatgcgtg gttggcgatt gtataaacgg caacggcacc tataaattgg
     2821 aaaatggtga tatgtatacc ggcaattgga taaatggtgt gcgcgaagga tatggcagat
     2881 atgattgggc gagtggcgca tattatgtcg gtaattttaa aaataatatg ctcgatggcg
     2941 aaggtgcttt ttatggtgtc gacggaacaa aaatgattgg catttttatt aaaaacaatt
     3001 atgcaggaac tgctgccgat acagcaaacc ggaaaattta ttcacccgat gatgaaatac
     3061 aaaaagcata taacgacatc aaaaaacgcg actcactggc aaaggcaaca acgcttgcaa
     3121 aggccgagcg cgttgagttc tgctcgttgc tgcgcaggct gacaacagat tttccaaatc
     3181 aattcagcag ctttcttgga gaacgccagg aaatagtgtt gagtaatagt aaaaattggt
     3241 atgcaacaat tatggctacc ggttcacttg aagccggtgt gaatagtttt tcaggaaaac
     3301 ctgagcgatc ttattataat attttattcg aaagtgttga ttccgtttct gcgcttgcac
     3361 agtatgcaca atatgttgaa caagtgaaaa cttgcaataa tggttgttgt acgatggtgt
     3421 atgatgaaag tacatttcat gcaaaatctc aatccggtta ttcaactatt tgggccacct
     3481 tggcggtgaa ggaaggattg gaaccgctgt tgtataataa tctgataatt gaaattgaat
     3541 gcctcacaaa aatggctgaa ccggggtgga tcgttgtgct cagaatgtac gatgcgcaga
     3601 cgttgaaata gggttttgat ggtggttttc tttttctcgc taagtcgcga agtgggtaag
     3661 acgcaaagtt gctctccttc gattttctga tttggtatac ttttagctct ataaaagtta
     3721 aaatatgtga catgaaacag tttttcgctg ctattacatt ggtgtttttt gtgcaattgg
     3781 ctgatgctca gggatttgtg tttgattcgg tgggtgtgca gcatatgacc attggcaagg
     3841 tgatgttaaa aagcgacacc tgggtgatca gggttgaacg cggtggcggt tatgatgatt
     3901 tctggcctgt aaatctgccg gaaaaatatg ccattgccgg tcaggaagtg gtttttgagg
     3961 gcgctttggg aagaattccc atgcaggcgc gacttgttgg cacaccggta aaactgtcga
     4021 tgatacgtgt attgtatcgc accactccga gagatgatga taaaagtggc gagtccattg
     4081 cgaaagaaaa accaataaat cccgactccg ttggatatat cgaaaagcaa acgggtcgca
     4141 ttttgatggt gaatgatatg tacctgatag aaactaccac cgttgatgca aaaggaaatg
     4201 aagaagtgaa gcgctacgtg ccggattttc tgcctgacga tttcaaaatg gcaggtcagc
     4261 aaattacatt cagcgcaata attctcaaac acgaccctaa tgtgcgtatg atgggaacac
     4321 ctgtgaaaat caaagaattg atgatggaag aaaatacgcc tttcgatgca acacaattgc
     4381 aagaatcggt tgcaccatat tatccattcg attctgcagg tcgacttgtt gaaactaagg
     4441 caatggtgaa attaattgca gatgtatata tacttgaagt gaccatcgac aatcaaaata
     4501 atatcacgcg atatattcca gccatacttc ccgaatcgtt taaaaaagaa ggaaccattg
     4561 taatgatcag cggcaccatc ggaaaaatac cacccaatgt acgcatgatg ggaacgccgt
     4621 tgacattaga aagtataagg ttggtggagt agttgggttc atgcaaaatc gcaatgggca
     4681 tgcgcttctc attattcagt gtattagctt ctggaattcg tatcgggtat cgggtatcag
     4741 gtatcaggta tcaggtatca ggtatcaggt aaaaattcac gaatcacgat tcacgattgg
     4801 tagtgagact aatgcaatta ttcgctcgcg ttgcgagcga atttgcatag tgaccactca
     4861 ccactcacca ctcaccactc acttcccctc agctattgtg attttcattt tcacatcctc
     4921 agcggggcga tcgccgtaca ttgtttttac tgcagcgatt ttatcgatta cttcaaggcc
     4981 ttttaccact tcaccaaaaa cggtgtaaga gccgtcgagg aaaggtgttc cgccgatggt
     5041 ttcgtatact tttttttgtt cgggagaaaa attcacgcca taaccgcgag agatattatt
     5101 taattcagct tcggttgaac gcttgccttg aacaatataa aactgagagc cggaacttgc
     5161 acgtgaaggg ttcacctgat cgccttgacg tgctgcggcg agagcgcctt ttttatgata
     5221 taatcccggt ttgatctcag caggaatttt ataatttggt ccgcctgcac ctaattgaac
     5281 attgggtcct gcattgcggg aatccggatc gccaccttga atcataaaat cttttatcac
     5341 gcgatgaaat aacaggtcgt tataataacc ttcttttacc agcttgataa aattatcgcg
     5401 gtgagccgga gtttcgttat acaattttac cagcatgtcg ccataagtgg tgctgatatt
     5461 tacataatac actttgttgt cgtccattgt atctgttgtt gttgtttgcg attgaatttg
     5521 ttcgggagtt gtagttgaga tcaccgtatc tgttttttgc ggaacgatgt cttctgttgt
     5581 ttctgatttt ttttcactgc aattagaaag taaggccacg cagcaaatgg caaaaatgat
     5641 ctgtttcata ataatttatt ttcttcgaaa tagcggataa tatgttgttt gataatgatt
     5701 tcctgttcag cagccgacca cgccggtaat tcttttgtcg cttcccacat gggccattga
     5761 tcatcgtccc atcctgccag gcgataatag ccgtcagcac tcaacaatcg gcatactgct
     5821 atatgcatga ggtcttgctt ttgttccttg ctgaatttgt cttccacctt acccaattcg
     5881 ttcatcccaa tcaagaatag aatagtttcc agctcaggtt ttttaccgaa ttggtcgcgc
     5941 aaacgctgca ataccgctat ccaccgggga tcaaaatcga taggtatctc ttcatgtgtc
     6001 agcgacatgc tgcaaagata gggcatcctg atgtttggaa ggtatcagcg gcgcacagga
     6061 atgcatattc gagatgacag ctatcttctc aaatcacaaa gcaataagga aactcaaacc
     6121 ttgtctatag gagcattgtc gctatagtaa gtatcctcac caattccatc agggtttgga
     6181 ccatatttgt ttgatccgtg ttgtcctaag gggaagagga gataaattgt ataaaatgga
     6241 atgagcacat accatccgct gttacccaga tcatgcgcgc gtttcacact ctgtgccaga
     6301 aaaaaccaaa ccaagggtat atataacaat agcagaaaca aggcgccttg cgatagcatg
     6361 ttagcgataa tggcattcat gataattgct acaacaatat acaatccgaa cgatttgccg
     6421 tattcggctc ggttgatacg tccttcaaaa gaaaatggtg ctgtgaacat gtggttgttt
     6481 tttacaaaca taataaaaca ttaccaaatt ttaatctaaa tcggaggttc attttcaaaa
     6541 agacgcaaac gtgcaacagt caatattgca tgatcaacaa ttttaatata acaatcagaa
     6601 atatacgcga aaacaaaata cgtgtgaaat ccaaataata ttagtcggaa tcaaaatgta
     6661 ttaaacttca aaagtgttat caaaacaata gtttacagtt caataacaga agtgagtttt
     6721 aataataact tttcgggcga tcgttttccc acagatttac acggagtacc atgccaatgg
     6781 tattggcctt tccttcattg ctttcgtatg catcacctga ccatgttgcc gggttcagtg
     6841 caacattgac gtcccaaaaa ttctgtttaa aaatcatcca ctgataatat cctttgaatg
     6901 taaatctgcc gccggcgtat tgcagataag gtgaaacacc aataatgagg tcccaattaa
     6961 tctcatagaa atcaccgggg tcattgccta ttgtatatgt gcgcgtgaaa ttttttacag
     7021 aaatagtgct tatatcaaac ccgccatata acccctgcac cttatccttc tggccggtta
     7081 attgataagc atatcccata ttaaacgtat tcatgcgata tttaaaatcg cgcgcatcgg
     7141 aagttgaaga cgactcggca gacacgttgc tggtatgtcc tataaattca aattccatta
     7201 aaaatttggg gaaatatgtt tcaacggcaa cattccatcc gcgaaaaaaa tgcacttcat
     7261 ccatgggctt agatagatat gggcgagtat cgttatatcg gcctattaca aaattcagtc
     7321 cgtcgttagt aatatgggtg ccaagtccat atccaactcc catgttgaat ttcgcatttg
     7381 attgtgcgcg caaaacattg catacaaata ccaataaaat aaatacaatg aaggttctca
     7441 tgttaatcaa taaaatgtta agagtatcgg tctacaactc ccgcggatgg tgtttgaata
     7501 atattaaaat ccggctagaa ttttattgta aaaatcgaag gtcagaaaat acccatcttt
     7561 aaaattggtc ataagcgtta cttcaatgat cacagactca tcaccctgaa cattgatgac
     7621 tgcaccaatg tcttgcatga gaaaccctac atttgaatat aatgtttctc ctgaagcaaa
     7681 ggccttggct gcgcccatag acgggaagta ataagcgtcg gaatagatat caggatacac
     7741 tttaacctgt ctgattgctt ccaacaaagc cgtatatgcc gcatagccat cctgctcatt
     7801 ggcatagtcg cccaaatagg cataataccc taatgaagga tctatcgcac ttaagttatt
     7861 ttctactgcg cccgggaatt gaagtgttgt tgtgtagcct tctgaaaagc tcacataggt
     7921 gcccgttacc aacttgaaac tgtcacgata agcctgatat aattcattga taaatttatt
     7981 tacggcagac gcatcaaaag atagaggtcc ctgcctaaat tcttctgata tcggagctgc
     8041 aacctgtttt tgcggctcat attgttgtcc cgccacaggc aaaaaagaaa aataaaaata
     8101 atcctcatag gcgccatcca gattgaatgt tcctgcatac caacctttag tatcaatgat
     8161 tgcctcgctg aggagataga tagtgtctcc aataaaattt ttctttttct taaagtgcgg
     8221 gtctttttca tttacatctg caatgatcac aaatgttccc tttggaagtg tgtcgatacg
     8281 cagtgtggtt gcagtagtct gggcagcgct gcgaaatcct gaaaatagaa atgccatgac
     8341 tactaaaagt gtggtggtgt gtttcacaat aatgtaatat ggatataaat ctacttgaaa
     8401 ttcacagaat accgtgaaca aggttcaaaa gagaattctt tacttcacat ctaaatttca
     8461 ggcattatga aaaaccttca gcacattccc aacattacgc cacagctaaa cgctgtaaga
     8521 gcgcgactcc aacacaccca agatctcatc caggcaagag ctgcagccca cgtattgcat
     8581 ccgaacgagt atcccttgcc ggcagatggc aatagtctgg aacatcattt tgttgaagtc
     8641 tatcaactgc tgtcagacaa aggcaaaaaa aacttcgaag aaaacgcaaa gctgatcatg
     8701 ggatatatga ctgccgatcg gagagcggaa ttgggtgacc tagctaacct gaatcacaaa
     8761 tcgccattaa gcactgttga acaagtgaaa caacttggca ctaatgatac catgcgtata
     8821 aaacaggagc atgtaacggc aaatgaaacc ttactgcgta aacacctgcc aaaatccttt
     8881 ccgggtatcg cttttgaagg ccctctaaga gaagtagatg tacgcggtca gaatcctcgc
     8941 actttaagtt tttttgttga tagtattcac tgtgaaaaaa caacagattt gcgaaaggat
     9001 gagatatcat tgtttggttt tacattcgat gcatttggac aaggaacaaa agttgaacaa
     9061 tttgaagttg gtaaatttaa aaaaggtgaa accattgcat taggcagcaa agctaaattg
     9121 agagatttcg atctgcgtgc cccgggattt tttccgcaat cattcttcac caatttattt
     9181 ttattcgaaa gggattgggt gaattttgaa aaaacaggta agcgcttgat gatgcttggc
     9241 gcggcattgt cggcaacatt cctgacggtg ggattctatt tacttacact ttcgccaatc
     9301 ccggtattgg gaaccgcact tggtattgca agtattgctt gcttattgat gacagctatt
     9361 tcgtatggat ttgcgttatt gattggcgcc atgtgtgaag acgtttccgg tggggcaggt
     9421 actgcattta cttttaatat acctccggta ataccggtag gaactgcttt caccgagacg
     9481 ctggatttta ctattgaagg cgggctattc ggcgttcaca aagcaaaata ttcagctcag
     9541 gtgcgatggg agcgcactgc ttaaaaaaaa ttcctacaac ttcgttactc gcatttccac
     9601 tctgcgattt ttcgcacggt tggattccgt gtcattggga gcaacgggtt tatcgggacc
     9661 aaaaccaacc gccataattc gcgattcgct gatgcctttg gaaacgatgt aatctttgca
     9721 cgctttcacg cgcttatagg acaatgattt attcattgca taatcgcctt cggaagaagt
     9781 gtgtccgctc aattcaattt cagctgatgg atttgtttgc atgaaagcaa caattttttc
     9841 caactcggtt ttcgattcag gacgcagatc tgcttttcct tgatcgaata atatattatt
     9901 tagcgtaata ctctgcccta ccgtgatatt attattatca aactttgctg attttccttt
     9961 ttccaaataa ataatgatag gatcattgtc atcaccctgc ccgatatctg ccgttgcact
    10021 tccgtcgata taaccttctg cggaggcgct caccacatcg aatccggtag gaatatctgc
    10081 aataataaat tcgccgttgg catttgtttt ggcaaagctt tgatttgaag taaataccgt
    10141 tgcatttgca ataggattct cagtatcctt tatcatcacc acccctcgaa catttccttt
    10201 acaactgttc tcgcgctttc tatttacaag cagtcgcacg aaatctaatg caaagccatc
    10261 gccaacacca ttcacttcat caatgctgat catacaattt ccgccattag caaaaagcgg
    10321 ataccaatct tcgggaaaag gaatagaaat tagttttccc accggccctg tctgatcaat
    10381 ggcgttcaac attttttctg cttcgagaaa atgtttgccg tttaccagaa taatatactt
    10441 cgaacaaaat gaaggcgcct gaaaatcatc gatgaacaat tgcagccaag cattttgcac
    10501 tgtagcatct ttcatttcat tcatgggtaa attccacaca acgggtttgt attgcatgga
    10561 tgggtcagcg aagctataac cttcagcgcc gcagggaaag tcttgcatgc catgccattt
    10621 cgacgacatc aatatcctgt caaagccggg aagatcgttg gcttggggct cccaaggata
    10681 ggcatgtgaa tccgtcatcc gtccgcaaaa ggggtcgaaa ccctccggcc acccaaatcc
    10741 gaggttatct acatcgccaa gtcgaatgat gtattccgct tccatggtgt ttttgaggaa
    10801 aacctcttgc ttttcccatt ctttatctga agaaacggtg gtcatttggg cgaaaatgac
    10861 attcgtcaat attgttccgg taaccagcag aagaaacggc aacgctttca tttctaaatg
    10921 tatttgagat acaaggtaag aaaaaaatca cgccgcaaca att
//
LOCUS       DAOQOU010000108        10869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3133_length_10869_cov_6.525522, whole genome shotgun sequence.
ACCESSION   DAOQOU010000108 DAOQOU010000000
VERSION     DAOQOU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_10869_cov_6.525522"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..454)
                     /locus_tag="PLM90_09810"
     CDS             complement(113..454)
                     /locus_tag="PLM90_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89712.1"
                     /translation="MQARKLMLPAMMLTLIIIVSSCSKSIDSCSGSVDCTGVNFSETI
                     QPLASTKCAISGCHGASFDSYSNMKSLADDGQLYSQVLDRQTMPDGGIDMSCEERAQF
                     NCWIDAGAPNN"
     gene            complement(726..1337)
                     /locus_tag="PLM90_09815"
     CDS             complement(726..1337)
                     /locus_tag="PLM90_09815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMZ89713.1"
                     /translation="MAFQLPALPYAFTALEPHIDARTMEIHHDKHHAAYTNNLNNAIA
                     GTEWESKTIEEILANVSKISMPVRNNGGGFYNHNLFWEIMGPNGGGAPTGDLMAAIET
                     HLGGWEKFKESFSNAATTRFGSGWAWLIVTPDKKLAVTSTPNQDNTLMDIADVKGTPI
                     LGLDVWEHAYYLNYQNRRPDYISAFFNVINWDVVTAKFNSAMK"
     gene            complement(1435..1878)
                     /locus_tag="PLM90_09820"
     CDS             complement(1435..1878)
                     /locus_tag="PLM90_09820"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ89714.1"
                     /translation="MGIIQDPDAFFMQEALKLATKALEEDEVPVGAIIVSGGQIIAKA
                     HNLVERLHDPTAHAEMQAITAACNHFQSKYLNECTLYVTLEPCPMCAAAAQWAQVGRV
                     VFGASDPKMGYRRFGHMLHPRTEVLEGVESERATAMLKQFFSGKR"
     gene            2062..3402
                     /locus_tag="PLM90_09825"
     CDS             2062..3402
                     /locus_tag="PLM90_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ89715.1"
                     /translation="MNRFAILACFLTISGWVYGQRMLSLPDCVKLATEQNITLRQGAT
                     GVEFTSKAADQSRLLLLPNLNAGASYFWNFGYTIDPVTNLPLSNNFSANSYQLTAAMN
                     LFSGGSVANTIKKSKADLEVAGMNYQSTIENVQFQVIAAYLAVMFAEEQTKVAEQKIA
                     TTELQLSNSRKLVAAGSLPEGNILAIEAQLAQDELSLIQARNQVDKTYLDLKLLLQID
                     ADEKVSVIYPDVTRFNQLLTQDAPDAKMVADYAIANKASIKTYDYQLISDELARNIAA
                     AGAYPSLSLIGQVSTNYSSASYPQFGIEADPFKEQIDNNLSQVVGLSLTIPLFNNGQI
                     LLSKQNADLAIINTQLNRELAVNTMRQNVTQAVNDLKAAQASYLASMKNYEAAVSAFD
                     FAEKKFNAGTASSFDYTNALNTKAQSESFLIQSKYDLIFKAKIVDYYLDKPIDF"
     gene            3412..4707
                     /locus_tag="PLM90_09830"
     CDS             3412..4707
                     /locus_tag="PLM90_09830"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ89716.1"
                     /translation="MRALKIVGIIVVVLLVVVLIGNNKGWFKQGGGTPVTADKVEKRT
                     VVETVTASGKIYPVSQVAIAAEISGEIVDLPVKEGDKVKEGDLLMRINPDLYESQVEQ
                     AQAALDNTKAQLSTARARVVQAKLQNDNAKIAFDRSSQLLKDKVISQAEYEQAQLAYQ
                     TAQAEYQIAQESVTAMEYTVKSSEAMLKEMRNNFKRTAIYAPASGTVVGLNKKKGEKV
                     LGTIQMTGDQLMTIADLTNMEIQVDVSENDVLRIDVGDTAEIEVDAYLNRTFKGVVFQ
                     IANSAGSSGSVLSAVTDQATNFKVKIYILPESYKDLMDANGDKYPFYPGMSATVEIKT
                     EVSRDVLAVPIQAVTAREDTSNTDKNKPVKEIVFLMDGDKVKEVTVETGLQDDAYIVI
                     KKGLSEGDRIVSGPYNTITNVLKDGDPVSVDKKKEEPKK"
     gene            4878..5375
                     /locus_tag="PLM90_09835"
     CDS             4878..5375
                     /locus_tag="PLM90_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ89717.1"
                     /translation="MAQMEIKGAEHGKRTRKQLFVDLTPMVDLAFLLISFFMLTTVLQ
                     ENKAIRLNMPETEGPDTKISMDRLITLIANDNNRVWYYYGDKPENGAYTDLTASGVRN
                     VLYQFHDKVISEQNREPICLIKFTGKADYNSMVGLLDEMDITDYNIYAIQDLTESELA
                     IQALK"
     gene            5435..6241
                     /locus_tag="PLM90_09840"
     CDS             5435..6241
                     /locus_tag="PLM90_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMZ89718.1"
                     /translation="MYNPETYLNATINDIVFEDRNKAYGAYQLRKNESRYVTYAMCVG
                     LLLTLMMTVFSLKANRSEEIVTGPIVIACPTDVDFPPLVKKIELPQPELPKPVAQRPT
                     VAYTTMVAKDDNAVTDDKVTTIDDLNGKDISTKNNDLPPTDENNPLIEKRTVVTEPVV
                     KKKEIIQFPEQAPEFNGGWTALQEYIAKKIRYPKGSSYIDAEGTVYVSFVVFDDGTIG
                     EVSTVRGIGMGYDEEAEKVIASMPKWFPGKQNGNPVNVRMTLPIKFQTLD"
     gene            complement(6315..7037)
                     /gene="rnc"
                     /locus_tag="PLM90_09845"
     CDS             complement(6315..7037)
                     /gene="rnc"
                     /locus_tag="PLM90_09845"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMZ89719.1"
                     /translation="MLSGFRKIYNCYLGNEQVFCRKLKSITGITPINHAVYKLAFQHA
                     SIHNSVHANNERLELLGDAVIDLVIADFLFKKYPFKSEGFLTEMRSKIVSREQLNRVA
                     LKIGLGEMMQVRKGGTDLTKSNVLGNALEALVGAIYVDAGYAKAYHFFMHKILIPHYE
                     IDELESLVVNFKSKIIEWAQKNGHEIRFELIEEHEQKGKRMYKMGLMFNAELIAENVD
                     YSKKRAEQKASEIACRKLGIHE"
     gene            complement(7042..8295)
                     /gene="fabF"
                     /locus_tag="PLM90_09850"
     CDS             complement(7042..8295)
                     /gene="fabF"
                     /locus_tag="PLM90_09850"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HMZ89720.1"
                     /translation="MALRRVVVTGLGALTPVGNSVHEYWEGLVAGKSGADFITRFDAT
                     NFKTRFACEVKNFDPTSVIDKKEVRRMDPFLHYAMAAVEEAVKDARVDASAVDVDRVG
                     VIWASGIGGLKTFEEEVSYFAKGDGTPRFNPFFIPKMISDIAAGMISIRYGFRGVNFA
                     TVSACASSTHSLIIAADCIRLGRADMIVAGGSEAAVTAAGVGGFNAMKALSERNDSPQ
                     TASRPFDVDRDGFVLGEGAGALILEEYEHAVRRGATIYAEYAGGGMSADAYHMTAPHP
                     DGLGAQLVMRDAFRDANLKAEDIQYVNVHGTSTPIGDPQEIKAIQSIFGEHAYQINIS
                     STKSMTGHLLGAAGAIEAIASIMALNNGVIPPTINHFTDDPVFDTRLNLTFNKAQNRE
                     LNAVLSNTFGFGGHNASVIFKKLSN"
     gene            complement(8310..8546)
                     /locus_tag="PLM90_09855"
     CDS             complement(8310..8546)
                     /locus_tag="PLM90_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMZ89721.1"
                     /translation="MSEIAEKVKKIIIDKLGVDESEVVPEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAEGITIVGDAIAYLEEHVK"
     gene            8673..9143
                     /locus_tag="PLM90_09860"
     CDS             8673..9143
                     /locus_tag="PLM90_09860"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HMZ89722.1"
                     /translation="MLPLSCELQLVARRKLKIDPDFDFLLAGLATPLQDYRLAWWINK
                     VLHKELSRVDDLVVTDAETRRQTSFARFDYPEELTRTVFHLLQNRVGSAYLMPELKEF
                     DYLLMIKGSYYRTKQAGILKKLREIEQMQASVFINPENLRSRNHLIIETKIAGW"
     gene            9172..10599
                     /gene="pyk"
                     /locus_tag="PLM90_09865"
     CDS             9172..10599
                     /gene="pyk"
                     /locus_tag="PLM90_09865"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMZ89723.1"
                     /translation="MKRGHNKTKIIATLGPVSANPEMIRKLIHAGVDVFRLNFSHGTH
                     ELHKQTIALIKEANRELNTHVSILADLQGPKIRLGEVLPLTVLKDGDIFRFTTHPQIG
                     NKEVAYITYPNFPKDVKVGETVLIDDGKIELKVLETNNHDTVVTKVVFGGPISSKKGV
                     NLPDTQVSIPSLTEKDHADLMFALGEGANWIALSFVRKAEDIIHLKNIIRHHGYHSRV
                     IAKIEKPEALKNIDEIIDATDAIMVARGDLGVEMPVELVPFTQKDLCRKCLAKATPVI
                     IATQMMESMIEHPHPTRAEVTDVANAVLDGADAVMLSAETAAGKFPEKVIKTMVRIVE
                     QAEKESVVYDRRHIPDKRSKTYLSDAICFNACNIAAEVHAAAIMGMTKTGYTAFMVSS
                     CRPKSRIYIFTNNPEVVNILNLLWGVEAFFYDGMRGTNNTFREVQQHLVEQGKLKQGD
                     TVINLGTMPVKEMARTNTLKISQIL"
BASE COUNT         3262 a         2346 c         2322 g         2939 t
ORIGIN      
        1 agcatatcac aattagccaa cacgcagaaa tgcagggcac cattgcttac gaagtgatga
       61 ccggcatttc acaacgtgtt aaacgcgttt actggaacga ataaaatttt gattagttat
      121 tcggcgcgcc agcatctatc cagcagttga attgcgcgcg ttcttcgcaa ctcatatcaa
      181 taccaccatc aggcatagtt tgacgatcga gaacctgcga atatagctgt ccgtcatcag
      241 ctaaactttt catattcgaa tagctgtcga acgaagcacc atggcagccg ctgatagcac
      301 atttggtaga cgccagcggt tgtatagttt cactgaaatt tacacccgtg cagtcaacag
      361 agccgctaca agaatcaata gatttagaac aagaagaaac aataataatg agtgtgagca
      421 tcatggcggg gagcattaat ttcctcgctt gcatagttaa taggttactt gaaatcataa
      481 acgttgagac ttgaaaaaat tgtatgtcga tacagtgatt tattttcgac aatacaaagc
      541 tgaagttttc aagtatctca accaatgaca aaaattataa gtttccaaga aaattgaatc
      601 cataaacaac gcttcttatt tagaatatgt ataaacatac aattgaaatt gctattttct
      661 tcaactatat gacataaaaa acccccgtat cgctacgagg gtttccggtt atgttagcag
      721 atgaattatt tcatcgcgct gttgaatttt gctgtcacca cgtcccagtt gatcacattg
      781 aagaatgctg aaatatagtc gggacggcgg ttttgataat tgaggtaata agcatgttcc
      841 catacatcaa gaccgagtat tggtgttcct ttcacgtctg caatatccat taaggtattg
      901 tcctggttgg gtgttgaagt cactgccagt tttttatccg gagtaacaat caaccatgcc
      961 catccacttc caaaacgagt agttgccgca ttgctgaacg attctttgaa tttttcccaa
     1021 ccgccaagat gtgtttcaat agcagccatc aaatcacctg taggagcgcc gccgccattt
     1081 ggtcccataa tttcccaaaa taaattgtgg ttataaaaac caccgccgtt attgcgcaca
     1141 ggcatcgata ttttcgatac gtttgccaat atttcttcta ttgttttgct ttcccattct
     1201 gttcctgcaa ttgcgttgtt caggttgttg gtataagcag catgatgctt gtcgtggtgg
     1261 atctccatcg tgcgtgcatc gatatgtggt tccaatgctg tgaatgcata cgggagagcc
     1321 ggtaattgaa atgccataag tatatctgtt gttttgtgaa taaatagttg tttacaaaga
     1381 gatgtaaaga taaaacacca gccatgaaaa gcagttcgct aaaagaatat taaatcatcg
     1441 ttttccgcta aaaaactgct tcaacatagc cgtagcgcgc tccgattcca cgccctccag
     1501 cacttcagtt cgagggtgca acatatggcc aaaacgcctg taacccattt tagggtcact
     1561 tgctccgaat actacccttc ccacctgggc ccattgcgcc gcagcagcac acatcggaca
     1621 aggttcaagg gttacataca aagtgcattc gttgaggtat ttagactgga agtgattaca
     1681 agcagcagtt attgcctgca tctcggcatg cgcagtcggg tcgtggagcc gctctaccag
     1741 gttatgtgct ttagcaataa tctgtcctcc ggaaacaata attgcgccca ccggcacctc
     1801 atcttcctcc aaagccttag tagccagttt caaagcctcc tgcatgaaaa atgcatccgg
     1861 atcctgtatg attcccatgg cacaaaatta agcgcaacat attaatgagc ccttaaagtc
     1921 tccttataat gccaaaaaat gccttcaaaa gcacctaata cctgtaactt tttgccctga
     1981 ggaaccattt atctttaaaa agtcctaatt tgtcgaatat ccgcattgcc tccccaaaaa
     2041 gtaccctaat tttgacctgt catgaataga ttcgccattt tagcatgttt ccttaccatt
     2101 tcaggttggg tttatggcca gaggatgctt tcgcttcccg attgcgttaa actggcaacg
     2161 gagcaaaata ttactttacg acaaggtgca acgggtgtag aatttacatc aaaagctgct
     2221 gatcaaagtc gtttattgtt gctgccaaat ttgaatgctg gtgcctccta tttctggaat
     2281 ttcggttaca ctatcgatcc ggtaactaac ttacccttgt ctaataactt ctcggcaaac
     2341 agttatcagc ttactgcagc catgaattta ttcagcggtg gcagcgttgc caataccatt
     2401 aaaaaaagca aagctgatct ggaagttgcc ggcatgaatt atcagtcgac cattgaaaat
     2461 gtgcagtttc aagtaatagc cgcatatctg gcagtgatgt ttgccgaaga acaaacgaag
     2521 gttgctgaac aaaaaattgc taccactgaa ttgcaattat ccaattccag aaaattagtt
     2581 gccgccggtt cattgccgga aggaaatatt ctggccattg aagcacagtt agctcaagat
     2641 gaattatcgc ttattcaggc gcgcaatcag gtagacaaaa catatctcga tctcaaatta
     2701 ttgttgcaga tagatgctga tgagaaagtg tctgttatat atccggatgt aactcgtttc
     2761 aaccaattat taacccaaga tgccccggat gccaaaatgg ttgccgatta tgctatcgcg
     2821 aataaagctt ccataaaaac atatgattat cagcttatca gcgatgagct tgccagaaat
     2881 atagctgcag caggggccta cccttcactt agtttgattg gtcaggtatc gacaaattat
     2941 tcgagtgctt cctatccgca atttggaatt gaagctgatc cttttaaaga acaaatcgac
     3001 aacaacttga gtcaggtagt aggattgtcg ctcaccattc cgcttttcaa taacggacaa
     3061 attttgttga gcaagcaaaa tgccgatctc gctattatta atacgcagct caaccgagag
     3121 cttgccgtta ataccatgcg tcagaatgtt actcaagctg tcaacgacct caaggctgca
     3181 caagcatctt accttgcttc aatgaaaaat tatgaggcag cagtaagtgc attcgatttt
     3241 gcagaaaaaa aattcaatgc aggcacagct tcttcttttg attacaccaa tgcattaaac
     3301 accaaagctc agtctgaatc atttttgatt caatcgaaat acgatctcat ttttaaagcc
     3361 aagatcgttg attattattt agataaacct atagattttt aattgaatat tatgcgcgca
     3421 ttaaaaatag ttggcatcat tgtagttgta ttactggtag ttgtattaat cggaaacaac
     3481 aaaggctggt ttaaacaagg cggaggcacg ccagttacgg cagacaaagt tgaaaaacgc
     3541 actgtggttg aaaccgttac tgccagtgga aaaatttatc ccgtaagtca ggttgccatc
     3601 gctgccgaga tctcaggaga aattgttgac ctcccggtaa aagaaggcga caaagtaaaa
     3661 gaaggtgatt tattgatgcg catcaatcct gatctttatg aaagtcaggt agaacaggca
     3721 caagctgctc ttgataatac aaaagcacaa ttgtcaactg cacgggctcg cgtagtgcaa
     3781 gcgaaattgc aaaacgataa tgcaaaaatt gcttttgatc gttcatcaca attgttgaag
     3841 gacaaagtaa tttctcaggc tgaatacgag caagcacaat tagcgtatca aactgcgcaa
     3901 gcggaatacc aaattgcaca agaaagtgta acggcaatgg aatataccgt aaaaagcagt
     3961 gaagccatgc ttaaagaaat gcgcaacaat ttcaagcgca ctgccattta tgcacctgct
     4021 tcaggaactg ttgtaggatt gaataaaaag aaaggagaaa aagtattagg aactattcaa
     4081 atgaccggcg accaattgat gaccattgcc gatcttacca atatggaaat acaggtcgat
     4141 gtatcagaaa atgatgtgtt gcgtattgat gttggtgata ctgcagaaat agaagtagac
     4201 gcttatttaa atcgcacgtt taaaggcgtg gtatttcaaa ttgccaattc tgccggcagc
     4261 agcggctcgg ttttgtcggc cgtaactgat caggctacca acttcaaagt gaaaatttat
     4321 atccttcctg aaagctacaa agacctgatg gatgcaaacg gcgataaata tccgttttat
     4381 ccgggcatga gcgccaccgt tgaaattaaa acggaagttt ctcgcgatgt attggcagtt
     4441 ccaatccagg cggtaacagc aagagaagac acttcaaata ccgataaaaa taaaccggta
     4501 aaagaaatag ttttcttgat ggatggcgat aaagtaaaag aagtaacggt tgaaacaggt
     4561 ttgcaggatg acgcttatat cgttattaaa aaagggttgt cggaaggcga ccggattgtc
     4621 agtggtccat ataatactat caccaatgta ctcaaagacg gcgatccggt gagcgtagat
     4681 aaaaagaaag aagaaccgaa gaaataatcc aataaattta attcaatgat tttagcgaca
     4741 gttgcaatag tagctgtcgc tatttttttg tgtatggcat ttgcagcttg caatttccta
     4801 ttgtcatatt cctatcagat ttcatatttt gatattgccg tatatcagcc tggcgtttta
     4861 ttatctaaac atgtcctatg gcacaaatgg aaatcaaagg tgctgagcat ggtaaacgca
     4921 cccgcaagca attgtttgta gacctcacgc ccatggttga tctggcattt ctgctgatct
     4981 cctttttcat gcttacaacc gtattacagg aaaacaaagc catcaggtta aacatgcctg
     5041 aaaccgaagg tccggataca aaaatttcga tggacaggct tattacgctg attgcaaacg
     5101 acaataatcg cgtttggtac tactatggcg acaaaccgga aaacggcgca tacaccgacc
     5161 tgacggcatc gggcgtacgc aatgtgttgt atcaatttca cgacaaagtg atatcggagc
     5221 aaaatcgaga acccatttgt ctgataaaat tcaccggcaa agcggattac aacagtatgg
     5281 ttggacttct cgacgagatg gatattacag attataatat ttatgccatc caggacctca
     5341 ccgaaagtga acttgctatt caggcattaa aataaataga cggcatttat ggaaaaatga
     5401 atacgctttc atcataccat taaatcgcac ctatatgtat aaccctgaaa cctatctcaa
     5461 cgccacgatc aatgatatcg tgtttgagga cagaaacaaa gcttatggcg cctatcaatt
     5521 gcgcaaaaac gaatcgcgat atgttaccta cgccatgtgt gttggcttat tgctaaccct
     5581 gatgatgacg gttttcagtt tgaaggcgaa tcgctctgaa gaaattgtaa caggtcctat
     5641 cgttatcgct tgtccaacgg atgtagattt tccgccgttg gtaaagaaaa ttgaactccc
     5701 tcaaccggaa ctacctaaac ctgttgcgca aagaccaact gttgcttaca caactatggt
     5761 agcaaaagac gacaatgctg ttaccgacga taaggtaaca accatcgacg atctgaacgg
     5821 caaagacatc agcacaaaaa acaatgatct gccaccaaca gacgagaaca atccgctgat
     5881 tgaaaaacga acagtggtta cagaacctgt tgtgaagaaa aaagaaatca ttcaattccc
     5941 tgaacaggca cctgaattca atggcggatg gactgcatta caggagtata ttgcaaaaaa
     6001 aatacgctat ccaaaaggca gtagttatat cgatgccgaa ggaacagtat atgtatcatt
     6061 tgtagtgttt gatgacggta ctatcggtga ggtaagcacc gttcgcggaa tcggcatggg
     6121 ctatgatgaa gaagctgaaa aagttatcgc ttccatgcct aaatggtttc ccggaaaaca
     6181 aaacggcaat ccggtgaatg tgcgcatgac actacctatt aaatttcaga cacttgatta
     6241 aatcgagatc aatcgctgac atagaagccc gggaattatt ccgggtttct tttttttgta
     6301 gcagggcgaa ataattattc gtgaattccg agtttacggc aggcgatttc ggaagctttt
     6361 tgttctgcgc gttttttaga atagtcgaca ttctctgcaa tgagttctgc attgaacatc
     6421 agtcccattt tatacatgcg ttttcctttt tgttcatgct cttcgataag ctcgaagcgt
     6481 atttcatgac catttttctg agcccattcg atgattttac ttttgaagtt caccaccaaa
     6541 ctttcaagtt catcaatttc gtagtgcggg atcaatattt tatgcatgaa gaagtggtaa
     6601 gccttagcat aaccggcatc cacatatata gcacctacca atgcctcgag cgcatttcct
     6661 aatacattac ttttagtgag atcggtaccg cctttgcgca cctgcatcat ttctccgaga
     6721 ccgattttta atgcaacgcg attgagttgt tcacggctaa cgatcttgct gcgcatttcg
     6781 gtaaggaaac cttcgctttt aaatggatat ttcttgaaca gaaaatctgc tatcaccaga
     6841 tcgataacgg catcaccgag caattcgagt cgctcgttgt tggcatgaac cgaattatgg
     6901 atagaagcgt gttgaaaggc aagcttgtat accgcatggt taatgggagt gatgcccgta
     6961 atgcttttca gcttacgaca aaacacctgt tcatttccca ggtaacagtt gtagatttta
     7021 cgaaatcccg atagcaagca atcagttact caatttttta aatatcaccg aggcattatg
     7081 accgccgaag ccgaaggtgt tgctgagaac agcatttagt tcgcggtttt gtgctttatt
     7141 gaaggttaaa ttgagtcggg tatcgaaaac gggatcatcc gtaaagtggt tgatcgtagg
     7201 agggattacg ccattgttca gcgccatgat actggcaatt gcttcaatag cgcccgcggc
     7261 accaagcagg tggcccgtca ttgatttggt tgagctgata ttaatttgat atgcatgctc
     7321 accaaagatg ctttgtatgg ctttgatttc ctgtggatca ccgataggcg tggaagtacc
     7381 atgcacattg acatactgaa tatcttcagc tttcaaattg gcatcacgga aagcatcgcg
     7441 cattaccaat tgagcgccta atccatcagg gtgaggagca gtcatatggt atgcatcggc
     7501 actcataccg ccaccggcat attcggcata aatggttgcc ccgcggcgaa cggcatgttc
     7561 atattcttcg aggattaacg ctccggctcc ttcacccaac acaaagccat cgcgatctac
     7621 atcgaaaggg cgtgatgctg tttgcggact gtcgttgcgt tcgctaagcg ctttcatggc
     7681 attaaatcca ccgacacctg cagcggtaac tgcagcctcc gatccgccgg caacaatcat
     7741 gtcggcacgg ccaagtcgga tacagtcggc agcaatgata agagaatgtg tactggaagc
     7801 acaagcagat accgtagcga aattcacccc tctgaaacca tagcgaatcg agatcatgcc
     7861 tgcggcgatg tcactgatca tctttggaat aaagaaaggg ttgaaacgcg gagtaccgtc
     7921 gcctttggca aagtaggaaa cctcttcttc aaaggttttg agtcctccga taccactggc
     7981 ccagataacg cctacgcggt ctacatccac ggcagaagcg tcgacacgtg cgtctttcac
     8041 cgcttcctcc actgccgcca tggcataatg caggaatgga tccatacgac gtacctcctt
     8101 cttgtcgatt acggatgtag ggtcgaagtt ttttacttcg caggcaaatc gggtcttgaa
     8161 gttagtggcg tcaaaacggg taataaaatc agcaccgctt ttgccggcca ccaacccctc
     8221 ccaatactca tgtacagagt tgccaacagg cgtcagagcc cctaatccgg taactacaac
     8281 tcttctcaaa gccatgagac ggatctaaat tacttaacgt gttcttccag gtatgcaata
     8341 gcgtcaccta caatggtgat accttcagcc tgttcgtcag gaatagagat gttgaattct
     8401 ttttcgaatt ccataatcaa ctcaactgtg tcgagtgagt cagcacccaa gtcattggtg
     8461 aagctggctt ccggaactac ttcgctctcg tcaacgccca gtttgtcaat gatgatcttc
     8521 tttacttttt ctgcaatttc agacattgta tttaggtttt aatagcactc cccctttaag
     8581 gcttttcgga gtggttgtta aaaaataaag tgcaaagata aggcaatacc cgttgattgt
     8641 gcaagtcaga tgtatatcaa taatgcatag gcttgttacc tttgagctgt gaattgcagc
     8701 ttgtggcccg gcggaaactg aaaatagacc ctgactttga cttcctgttg gccggtttgg
     8761 ccacgccatt gcaggattat cgcctggcat ggtggatcaa taaggtgcta cataaagagc
     8821 tgtcgagggt cgacgatttg gtggtgacag acgctgaaac ccgccgccag acgtcgtttg
     8881 cgcgcttcga ttatcccgaa gagctcaccc gaacggtgtt tcacttgctg caaaaccgcg
     8941 taggatcagc ttacctgatg ccggaattga aggagtttga ttacctcctg atgatcaaag
     9001 gcagctatta ccggactaaa caggcaggta tcctgaaaaa attacgcgaa atcgaacaaa
     9061 tgcaggcttc ggtgtttata aatcctgaaa acctacgttc caggaaccac ctgattatcg
     9121 aaacaaagat tgcaggatgg taaattttga taacaggcac gactatataa tatgaagaga
     9181 ggacataaca agacaaaaat catcgcaaca ctgggccctg tgagcgcaaa cccggaaatg
     9241 atcaggaagc tcatacatgc aggggttgat gtattcaggc tcaatttttc acatggcacc
     9301 cacgaactcc acaagcaaac gatagcactc attaaggagg ctaaccgcga gctcaatacc
     9361 catgtgagta tacttgccga cttacaggga ccaaaaattc gtctcggcga agttttacca
     9421 ttgacagtat taaaagacgg cgatattttt cgatttacta cacatccgca aatcggtaat
     9481 aaggaagtag cctatatcac ttatccaaat tttcctaaag acgtgaaagt tggtgaaaca
     9541 gtgctcatcg acgacgggaa aattgaatta aaagtactgg aaacaaataa tcacgatacc
     9601 gttgttacta aggtggtgtt cggcggaccg atatcttcaa aaaaaggtgt caatctacct
     9661 gatacacaag tatccattcc atcgcttaca gaaaaggatc atgccgattt aatgtttgcg
     9721 ttgggtgaag gcgccaattg gatagcattg tcgtttgtgc gcaaggctga agatattatt
     9781 catctgaaaa atattattcg tcatcacggt tatcactcgc gcgtaattgc aaaaattgaa
     9841 aaaccggaag ccttaaaaaa tatcgacgaa atcattgatg ctactgatgc catcatggta
     9901 gcacgtggtg atcttggtgt tgaaatgccc gtagaattgg taccgttcac acaaaaagat
     9961 ctttgcagaa aatgtttagc gaaagcgaca ccggtaatca tcgccactca aatgatggaa
    10021 agcatgatag aacatccgca tccaacacgt gccgaggtga cagatgttgc caacgccgtg
    10081 ctcgatggtg ccgatgccgt aatgctgagc gctgaaactg ctgctggaaa atttcccgag
    10141 aaggtaatta aaaccatggt gcgtattgta gagcaggcag agaaagaaag tgttgtttat
    10201 gatcgacgtc acattccgga taagcgctcc aaaacatatt tgagcgatgc gatttgtttt
    10261 aatgcatgca atattgctgc cgaagtacat gccgcagcaa ttatgggtat gacaaaaaca
    10321 ggctatactg cctttatggt ttccagttgc agacccaaat cacgtattta tattttcacc
    10381 aataatccgg aagttgtaaa tattttaaat ctgctctggg gagtagaagc atttttctac
    10441 gatggtatgc gtggaacgaa taatactttc agagaagtgc aacaacattt agtagaacaa
    10501 ggcaagctca aacagggaga taccgttatc aaccttggca ccatgccggt gaaagaaatg
    10561 gcgcggacaa atacattgaa gatttcacag attttataat aatataatag cgggatttta
    10621 aggcttgcac atcacgctcg gtttcgaccg catgcggagg aggagtttca tgttatcgga
    10681 agttaattgc gaaactaagt tcttcatgct ttctattgat cgaattaatt ctgcaaacca
    10741 caactttgct ccgcatcaca aatgtattcc accttttgaa gccaaaaggt ggagccaaaa
    10801 gctcagggct tctgcagctg catgacacag ccctaatgct tgcgtcatgg cccggcctca
    10861 ccccagccc
//
LOCUS       DAOQOU010000109        10803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3168_length_10803_cov_7.273260, whole genome shotgun sequence.
ACCESSION   DAOQOU010000109 DAOQOU010000000
VERSION     DAOQOU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_10803_cov_7.273260"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLM90_09870"
     CDS             <1..310
                     /locus_tag="PLM90_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89724.1"
                     /translation="SVFDNIPLTKLALDQGGYDWGFLAYAVGFGGSMVWFGSSAGVAI
                     SNMYPEARSVGSWVKNGWYVIVGYVIGYFILLLTLGWNPHPPHKPPVNSTTTSSSQID
                     "
     gene            complement(410..1114)
                     /locus_tag="PLM90_09875"
     CDS             complement(410..1114)
                     /locus_tag="PLM90_09875"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ89725.1"
                     /translation="MKKTTSIALLGAVLLSVSACKNAPDSDEALTGDAQEVTLTEGST
                     SVNINPATSSLEWIGTKMSTYHNGTVAIKSGTLLLKDGVITGGNFVIDMPTLKSVKDD
                     DMTNGKLTGHLQSPDFFDVAKFPEAKLEITSVKPNTTGASTGGDQPEINEYTVADPNY
                     MISANLTIKDVTKNIEFPAKVTVNNSGVSAVAKFNIDRKQWGIVYKGMPDDLIQDLIW
                     FGVSIKADAPVNTTSL"
     gene            1250..2020
                     /locus_tag="PLM90_09880"
     CDS             1250..2020
                     /locus_tag="PLM90_09880"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HMZ89726.1"
                     /translation="MITTLLTSLCIAMLFMTGAWLFSLYKRDMSVVDGFWGLGFLAIA
                     HHWFFRLEEAQLRQFAVVYLVTIWGLRLSAHIFIRSKGKPEDPRYTAFRNEWKENTWW
                     ISYFKVFLLQAVLCFVISLPVINVLAHHEEGFSCLNVVGLILWFIGFAFESTADYQLF
                     RFKKNPANKGQVMNKGLWYFSRHPNYFGEVCVWWGIFLVSIGSGLWYISVLGPILITF
                     LLLRVSGITMLEKRYEGNDAYSLYKKNTSPFFPWWPKK"
     gene            2113..3189
                     /locus_tag="PLM90_09885"
     CDS             2113..3189
                     /locus_tag="PLM90_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89727.1"
                     /translation="MYKREVIADYLYPDFNLDLISNYTIDSAGIQLTMRMNADLTWKH
                     ITDTLGTSRSVLHTRYANYPEAKSFIETAFSKDTLPNMGEGVLESSLVTPQGALTLYF
                     THDKKHNNRLYIYHAEENAIYIKDVPFGYEIGQNDTSVFYRTTKFKHEKVKYAAGTIV
                     SVGINDSKIEQPLFKHQSDRFQHCTFYADPYIAYWQLYSQSFYDCHTAFHNKIYIHYN
                     NSLTDSIQYVDEYIKPYLSLKNDTLSLVIYEFDTLLLKSFLSLYQLTSGQFIRSWKSQ
                     EWQEVVNENFSFIKNYRIVGSVIFVETERRVSSEDVTKSIEVPYVLSPNGLPFTKKGT
                     IPIITAFDLHSNAFLGYPQISIIN"
     gene            3342..3737
                     /locus_tag="PLM90_09890"
     CDS             3342..3737
                     /locus_tag="PLM90_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89728.1"
                     /translation="MSTVKKTIVPVLLSTVWISVNEFIRNQLVLQNYWTDHYNNLGIT
                     FPAEPVNGAVWGIWSLLFAVAILIISKRFSLRDTTLLSWLVGFVLMWVVIGNMGVLPT
                     GILIFAIPWSLVEAFGAAYISHYFTKPRA"
     gene            3737..4441
                     /locus_tag="PLM90_09895"
     CDS             3737..4441
                     /locus_tag="PLM90_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89729.1"
                     /translation="MLSATAASAILAYGGETFWKEAKNIEAEVSVRGWAFRLKWRPFF
                     DHVHIKLDAEHPFSQLTPIGSDLGVVAVLDGDDVHLEDLTGEISKFRSGAKHYFPYGR
                     RLLYWDDLDMGYFANYAFWNYFTLPKLLLRDDIKWIETKYGVLHAIFPDHIPTHCKEQ
                     QFTFDTTTGLLKQHDYTAEVISGLATAAHVIHEHQTKNGITFASRRIVTPRTKTGKAM
                     KAPVLIDITVHDLKIS"
     gene            complement(4444..5451)
                     /locus_tag="PLM90_09900"
     CDS             complement(4444..5451)
                     /locus_tag="PLM90_09900"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMZ89730.1"
                     /translation="MKIAVVGATGLVGQMMRRILEERNFPVTEMIPVATERSVGKKVK
                     FRGEDVEVVGMETAIAMRPQIALFSAGGNTSLEWAPKFEEVGCWVIDNSSAWRMDPTC
                     ALVVPEINAHDIVKYGRKIIANPNCSTIQMVMALNPLHKLYSIKRIVVSTYQSVSGSG
                     AKGVMQLYNERSSGCRVAENPAYPHQIDKNVIPQVDVFMENGYTKEEMKMHNETRKII
                     GSDNIMVSATTVRVPVSGGHSESVNVEFERDFSIDEVITTLQQTEGVVVEDDLANNVY
                     PMPINAEGKDEVFVGRIRRDYTQANTLNFWVVSDNLRKGAATNAVQIAEYLISSGIVK
                     D"
     gene            5579..8182
                     /locus_tag="PLM90_09905"
     CDS             5579..8182
                     /locus_tag="PLM90_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ89731.1"
                     /translation="MKSIYTLCLVMLACAVGQAQSFFDDFNDADISDWGGTPEYFIAN
                     GTGELQLNGDCAAGGDHYISQALPTLDSAVWSFQVHLDFDPSNSNHTRVFLQSDIADL
                     SGDVYGYFVRVGEDGTGDVVKLWRSNGASASVVITGTSPVAFSPVVGVKVVRTADAEW
                     QLYVDADGGTDYVLEGTDIDDTYNGGNYAGIECRYTSTRCTLFYFDDFSIDPLYIDVT
                     APAIQSVTVLSSTQLLVDFTENITLVTAETESNYTVTGGVGSPIDATRDVIDNSKVTL
                     TFLSEFPEGITLTLAVNGVTDDAGNITSGATANFSVYTTQLYDIVINELMADPSPPVA
                     LPEDEFLELYNTTDVDIDITGFTLSDASGPSDPFTSYILPAHGYVILCDDGDISSFLA
                     YSNVLPVAGFPSLNNDGDDMMLSDQNGIEIHRVTYTSDWYQNAIKADGGWTLEMIDPA
                     NPCQGIENWIASDAVEGGTPGAQNSVFGSNPDLVAPALLEVFPQTLDTLTLTFSEAVI
                     GENIITADIQITDETGASIAIAAIILNPDNDAKIGVVLSSPLSGGVIYTCTVMHAADC
                     SGNDILLFNTITFGIPEPVSAGDIVINEILFNPVTNGVDYVELYNRSNKIIDLSTLII
                     AELELLDSTVIDEFAYVTSDGQLLFPGNYICVTSDIEQVLAQYFTVDTGNFVKNNNLP
                     GYNDNEGIVVLYDATLNEIDHLHYFDDWHYALLDDENGVSLERVNYAFATQDENNWHS
                     AASDVHYGTPGYQNSMFGEIHAQATIGVEYPVFTPDGDGYHDLLVITYLTESEGFTGA
                     FRLFDAQGRKIKTLTTNELLSREGFITWDGVTDDGSKANSGIYIIYAELFNTEGVVER
                     FKLKCTLLRKQ"
     gene            complement(8183..>10803)
                     /locus_tag="PLM90_09910"
     CDS             complement(8183..>10803)
                     /locus_tag="PLM90_09910"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HMZ89732.1"
                     /translation="ATYPTIEYNQLNWFGFSLNGYQFLNYDIGAAADFVDQRSRSIVT
                     GEGLDNLMPAMMQEVQITKTNKGAVGNGFYADLEVVKEKNQREVPAPLGKIPMRNNFN
                     ETAFFFPQLETDSLGNVSFSFTMPDALTTWKFMAYAHTKDLQTGSAMASVITQKELMI
                     QANMPRFLREGDHITLAAKISNLTDKDISGSAQLELFNAVNMQPVNAVFENKQATVSF
                     TASAKGSTSVQWEVHVPEGIDAIVYRVKAGNETFSDGEENGLPVLTNKILVTESIPLW
                     VRGGDSKSFVFDKLLKSGNSSTLRNQNVTLEYTSNPAWYAVQALPYMMEYPYECAEQV
                     FSRFYANSIAAHIVNSDPEIKRVFDSWKKNPSSLVSNLEKNEELKSLMLEETPWVLQA
                     QNETERKKRVALLFDVNKMQSEQRAALQKLQKMQLPDGAWPWFSGYKDDPYITQYIVT
                     GLAHLERLSATEYNTAGEVDAMLRSAMPYCSANMYETYTRLEKNKVEKSSYVPDNLIL
                     QYLYAASYFDEKRFPLTKNEAEAKAYFIAQIKTYWTSYSLYQKGMIAIILNRNDQVKT
                     AQQIVASLKENSIKSEEMGMYWKSNIAGYYWYQAPIETQALMIEVFAEVANDKNAVDA
                     LQIWLLRQKQTTDWKTTRATAEACYALLSRGLNLLANTELANITIGNLQVKPEQTELG
                     TGYFKSTWLGTNINPAMGNITITPPANNLLSYGAMYWQYFEEMDKVTSAETNLKLKKQ
                     LFLEVNSASGPVLRAVDEKTELHVGDKVIVRIELKSDRDMEYVHMKDMRASGFEPTNV
                     ISSYKWQDGLGYYEATGDASTNFFISYLRKGTYVFEYPVRVSHKGSMSNGITSIQCMY
                     APEFSSHSEGSRVTVK"
BASE COUNT         2965 a         2332 c         2138 g         3368 t
ORIGIN      
        1 cagcgttttt gataatattc cacttacaaa actggcattg gatcagggtg gttatgattg
       61 gggattttta gcctatgccg ttggatttgg tggcagtatg gtgtggttcg gatcgtcggc
      121 aggcgttgcc atttcaaata tgtatcctga ggcgcgctct gtaggcagct gggtgaaaaa
      181 tggttggtat gtaattgtag ggtatgtaat tggatatttt attttgttgc tcacactcgg
      241 ctggaacccg catcctcctc ataaacctcc ggttaacagt actactactt cttcttccca
      301 aatagattaa aaaaagccgg aaagaaacaa ttcttcccgg ctataaaaaa caaccactac
      361 tctaattata tttctgttta gccgttcagc taaaaattta tttttcgaat tataacgatg
      421 tggtatttac gggagcatca gctttaattg aaacaccaaa ccatattaaa tcctgtataa
      481 ggtcatcagg cataccctta taaacaatac cccattgttt gcgatcgata ttaaatttag
      541 caacagctga aacgcctgaa ttgttcactg ttaccttggc agggaattca atattcttag
      601 ttacatcttt aatagtaaga ttggcggata tcatgtagtt tggatccgct accgtatatt
      661 cattgatctc aggttgatct cctccagttg atgctcctgt tgtatttggt ttcacagaag
      721 tgatctccaa ttttgcttcc ggaaattttg caacatcaaa gaaatcaggc gattgcaaat
      781 ggcctgtaag cttgccattg gtcatatcat cgtctttcac agatttcaag gtgggcatat
      841 ctattacgaa gttaccaccg gtgattacgc catcctttaa caataaagta cccgatttga
      901 ttgctactgt gccattgtgg taagtcgaca tcttagtccc tatccattcg agcgaactcg
      961 ttgcgggatt tatatttacc gaggtagacc cctctgtcaa tgtcacttcc tgtgcgtcgc
     1021 cggttaaggc ctcatcgctg tccggagcgt tcttacatgc cgaaacgctc aataataccg
     1081 ctcctaagag ggcaatagat gtagtcttct tcattgattt tatcaaattt gtatgtatat
     1141 acatacattt taacgccaaa gttcccaaaa agtttacgat ggttaaacat ttttattgaa
     1201 tggcggttat ttgcatattc aggacgtata tttgaatgaa atacaggcta tgattaccac
     1261 attactcact tccttatgta tagccatgct ttttatgacc ggcgcatggt tgtttagctt
     1321 gtacaagcgc gacatgagtg tggttgacgg attttggggc ctgggatttc tagccattgc
     1381 ccaccattgg ttcttcagat tggaggaggc gcagttgcga caatttgcag tagtgtatct
     1441 ggttaccata tggggattgc gcttgtcggc acatattttc atccgcagta aaggcaagcc
     1501 ggaagacccc agatataccg catttagaaa cgaatggaaa gaaaacacct ggtggatcag
     1561 ttatttcaaa gtatttcttt tgcaagcggt attgtgtttt gtgatttcat taccggtaat
     1621 aaatgtactg gcgcatcacg aggagggatt cagctgctta aacgttgttg ggttgattct
     1681 gtggtttata gggtttgcct ttgaaagcac tgccgattat caattattcc gatttaaaaa
     1741 gaatcctgcc aataaaggac aggtaatgaa taagggtttg tggtactttt cgcgtcaccc
     1801 caattatttc ggtgaagtat gtgtttggtg gggaatattt cttgtgagca taggttccgg
     1861 actttggtat atttccgttt tagggccaat actgattaca tttttattgc ttcgcgttag
     1921 cggtatcacc atgttggaga aaagatatga aggcaatgat gcttattctt tatacaaaaa
     1981 gaacacatct cctttttttc cgtggtggcc gaaaaaataa ttggctacaa tccacgtttt
     2041 ttatcgatgc gcttgctgct tataagccta tttctaccgt ggagtttcaa tttatatgct
     2101 caatgtgatc ctgtgtataa acgcgaagta attgccgact atttatatcc cgacttcaat
     2161 ctcgatttaa tatccaatta cacaatcgac agtgcaggaa ttcaattaac aatgcgcatg
     2221 aatgccgacc ttacttggaa gcatattacc gacacccttg gcacaagtcg ctctgttttg
     2281 catacgcgat atgcaaacta tcctgaggcc aaaagtttta ttgaaaccgc attcagtaaa
     2341 gataccttgc ctaatatggg cgaaggcgtg ttggaatcaa gcttggtaac accacaaggg
     2401 gcgcttactc tttattttac acatgacaaa aaacacaata atcgattata tatttatcat
     2461 gctgaagaga acgccattta cataaaagat gtgccattcg gatatgagat aggccaaaat
     2521 gatacaagtg tattttatcg cacaacgaaa ttcaagcatg aaaaagtaaa atatgccgca
     2581 ggaactattg tctctgtagg aataaatgac agcaaaatcg agcagccttt atttaaacat
     2641 caaagcgacc gctttcaaca ctgtacattt tatgccgatc cctacattgc atattggcaa
     2701 ctgtacagcc aatctttcta tgattgccat accgcctttc acaataaaat ctacatacat
     2761 tacaacaact cactgaccga tagcattcaa tatgtagatg aatatattaa accatatttg
     2821 tcgttaaaaa acgatacact tagccttgtt atttacgaat ttgatacatt acttctgaaa
     2881 tcatttttat cgctatatca actcacatcc ggtcaattta ttcgcagttg gaaaagtcaa
     2941 gaatggcagg aagtggttaa tgagaatttc tcatttatta aaaactatcg aattgtgggt
     3001 agcgtcattt ttgtcgaaac agaaagacgt gtttcttccg aggatgtcac aaaatcgatc
     3061 gaagttccct atgtactatc tccaaatggc ttgcccttta ccaaaaaggg tacaatcccc
     3121 attattacgg ccttcgattt gcacagcaat gcctttttag gttatccgca aattagcatc
     3181 atcaattaat tgggtttggg catattttgc tctgtcattt tttgttataa agtgaaatac
     3241 tattgacaat tgatttgcat ttgttaacat attgaaagcg caggaattat tagtttagcc
     3301 tctaatttcc ataaaacatc tcctcaccat taatttgcaa catgtctaca gtaaaaaaaa
     3361 cgattgttcc tgttttgctt agcactgttt ggatcagtgt taatgagttt atcaggaatc
     3421 agcttgtatt acaaaattat tggaccgatc attataacaa cctgggcatc acctttcctg
     3481 cggagcctgt caacggtgcg gtttggggta tttggtcttt attatttgct gtcgctattc
     3541 tcatcatatc gaaacgattt tcgttgcgcg acacaacact attgtcgtgg ttggttggat
     3601 ttgtattgat gtgggtggtg ataggcaata tgggtgtact gccaacggga atcttgattt
     3661 ttgccattcc atggagcctt gtggaggcat tcggagcggc atatatttct cattatttta
     3721 ctaagccgag agcctgatgc tgagtgcaac agccgcttcc gccatattag cttatggtgg
     3781 cgaaaccttt tggaaggaag cgaaaaatat tgaggccgaa gtgagtgtcc gcggatgggc
     3841 atttcgctta aagtggcgtc cgtttttcga tcatgtgcat atcaaattag atgccgaaca
     3901 tccattttca caacttacac ctattggaag cgaccttggt gtggtggcag tattagatgg
     3961 cgacgacgtg caccttgaag acctcaccgg cgaaatttct aaattcagat ccggggcgaa
     4021 acattatttc ccatacggca ggagattatt gtattgggat gatctcgata tgggttattt
     4081 tgcgaattat gctttctgga attatttcac ccttccgaaa ttattgctgc gcgatgatat
     4141 taaatggata gaaactaaat acggtgtttt acatgccatt tttcccgacc acatcccaac
     4201 acattgcaaa gaacaacaat tcacattcga cacaacaaca ggcttattaa aacaacacga
     4261 ttacaccgcc gaagttattt ccggacttgc cactgctgca catgtaatac atgaacatca
     4321 aacaaaaaac ggcatcacat ttgcatcgag aagaattgta acgccacgca ctaaaacagg
     4381 aaaagccatg aaagcaccgg tattgatcga cattaccgtg catgatctga agatcagttg
     4441 aaattaatct ttaacgatac ccgaagaaat cagatattcc gcaatttgta cggcattggt
     4501 tgctgcgcct tttcgaagat tatccgatac cacccagaag tttaatgtgt ttgcttgtgt
     4561 ataatcgcgg cgtattcttc ccacaaacac ttcatccttt ccttcagcat taattggcat
     4621 aggataaaca ttgtttgcaa gatcgtcttc caccactaca ccttcagttt gttgcaacgt
     4681 agtgataact tcatctattg aaaaatcgcg ttcaaattca acattcacgc tttcactatg
     4741 tccgcccgaa accggtacac gcaccgttgt tgcagaaacc atgatattat ctgagccaat
     4801 aatctttctt gtttcattgt gcattttcat ctcctctttc gtgtatccat tttccataaa
     4861 aacatctacc tgaggaatca catttttatc tatctgatgc ggatatgccg gattttcggc
     4921 aacgcgacat ccactgctcc tttcgttata cagctgcatc accccttttg ctccggatcc
     4981 gctaacagat tgataagtgc tgacaacgat gcgttttatg ctatataatt tgtgcaatgg
     5041 attcagtgcc attaccattt gaatggtaga acaattcggg ttggcaatta tttttctgcc
     5101 atatttaaca atatcatgcg cattaatttc aggaaccact aatgcacagg ttggatccat
     5161 gcgccaggca cttgaattgt caatcaccca gcagccaact tcctcaaatt tcggcgccca
     5221 ctccaatgaa gtattacctc cggcgctaaa gagggcaatt tgcggacgca tggcaatagc
     5281 agtttccatg cctacaactt ccacatcttc acccctgaat ttcactttct tgcccacgga
     5341 gcgctcggtt gcaaccggta tcatttcggt aacaggaaag tttctttctt ccagaattcg
     5401 gcgcatcatc tgccctacca ggcccgtggc accaactacg gcaatcttca tggcacaaag
     5461 ataaatgccc gtttcaggtt gccctctatt cctataaaga ttttctgcat ccggggcatc
     5521 aatttaacga atccggcttt ttcaagccat ttatgtaact ttcgggtctt tatttgccat
     5581 gaagtcgata tacacactgt gtttggtgat gctggcctgt gcggttgggc aagcacaatc
     5641 attttttgat gattttaatg atgccgacat ttccgattgg ggcggaacac ccgaatattt
     5701 tattgccaat ggcaccggag agctgcaact aaatggcgat tgcgctgcag gtggcgatca
     5761 ttatatcagc caggctttgc ctaccctcga ctctgccgta tggtcgtttc aggtgcatct
     5821 cgatttcgat ccgtcaaatt caaatcatac ccgcgttttc ctgcaaagtg atattgccga
     5881 tttaagcgga gatgtttacg gatattttgt tcgcgttggc gaagacggca ccggcgatgt
     5941 tgtaaaacta tggcgcagca atggtgcctc tgcaagtgtg gtgattacag gaacttctcc
     6001 ggttgcattt tctcctgtag ttggtgtaaa ggttgttaga accgctgatg ccgaatggca
     6061 attatatgtt gatgcggatg gcggaacaga ttatgtgttg gaaggaacag atattgacga
     6121 tacctacaac ggaggaaatt atgcgggcat agaatgcaga tatacctcta cgcgctgtac
     6181 gcttttttat tttgatgatt tctcaatcga cccgctatat atagatgtta ctgctcccgc
     6241 aattcaatcg gtaaccgtat tatcatctac acaattactt gtcgacttca cagaaaacat
     6301 aacactggta actgcagaaa ccgaatccaa ttataccgta acaggtggcg taggaagccc
     6361 aattgacgct acgcgcgatg taattgataa ttctaaagtt acacttacct ttttatcaga
     6421 atttcccgaa ggcattacac ttacacttgc cgtaaatggt gttaccgatg atgccggaaa
     6481 tatcacgagt ggcgctaccg ctaatttttc tgtttacact actcaattat atgatattgt
     6541 catcaatgaa ttaatggcag acccttcacc gcctgttgct ttgcccgaag atgaattttt
     6601 agaattatat aataccactg atgtagatat cgacatcacc ggatttacgc taagcgatgc
     6661 atccggtcca agcgatccat ttacatccta tatacttcct gcccacggat atgttatttt
     6721 gtgtgatgat ggcgatattt caagcttttt agcctatagt aatgtgcttc ctgttgccgg
     6781 atttccatca ctgaataatg acggcgatga catgatgctt tccgatcaaa acggaattga
     6841 aattcatcgc gttacctata cttccgattg gtaccaaaat gcaattaagg ctgatggcgg
     6901 ctggacgctt gaaatgatag acccggcaaa tccatgtcag ggaattgaaa actggatagc
     6961 atcagatgct gttgaaggtg gtacacccgg agcacaaaac tccgttttcg gcagcaatcc
     7021 cgatctcgtc gcgcctgcct tgcttgaagt ttttccgcaa acactcgata cccttacgct
     7081 tactttcagc gaagctgtta ttggtgaaaa cataatcact gccgatattc agatcaccga
     7141 tgaaaccggt gcgtcgattg ccattgccgc aattattcta aaccctgaca atgatgcaaa
     7201 aattggcgtt gtgttaagca gtcccttgtc gggtggtgtt atctacactt gcacagtaat
     7261 gcacgcagcc gattgcagtg gaaatgatat tcttttgttt aatacaatta cgttcggcat
     7321 ccccgaaccc gtttccgccg gcgatattgt aatcaacgaa atattgttta atccggttac
     7381 caatggcgtt gattatgttg aattatataa tcgctccaat aaaatcatag accttagtac
     7441 actgatcata gctgagttag aattgctcga ctcaacagtt atcgatgaat ttgcgtatgt
     7501 aacttctgat ggtcaattat tattccccgg aaattacatt tgtgtcactt ctgatatcga
     7561 gcaggttttg gcacaatatt tcactgttga caccggaaat tttgttaaga acaataacct
     7621 tcccggctat aacgacaatg aaggaattgt agtgttatat gatgctactt tgaacgaaat
     7681 agatcacctg cattatttcg acgattggca ctatgccttg ctggatgatg aaaatggcgt
     7741 atcgcttgag cgagtgaatt atgcctttgc aacgcaagat gaaaacaact ggcacagcgc
     7801 cgcatctgat gtgcattacg gcacacccgg ttatcaaaac tccatgtttg gcgaaattca
     7861 cgcacaggca acaattggtg tagaatatcc ggtatttact cctgatggcg atggttatca
     7921 cgatttgtta gtaatcacgt atctcacaga gtcggaaggc ttcacaggcg catttagatt
     7981 atttgatgct cagggcagaa aaataaaaac cctcacaaca aatgaactgc taagtcgcga
     8041 gggttttata acctgggatg gcgttactga cgatggcagt aaagccaatt ccggcattta
     8101 cattatttat gccgaattat ttaataccga aggtgttgta gaacgattca aattaaaatg
     8161 caccttgttg cgcaagcaat aattatttca cagtaacgcg acttccttca ctatggctgc
     8221 tgaattcagg ggcatacatg cactgaatgc ttgtgatacc attactcatg cttcctttat
     8281 ggctaacgcg aacaggatat tcaaacacat atgttccttt gcgtaaataa gatataaaga
     8341 agttagtgct tgcatcgccc gttgcttcat aatatccaag tccgtcttgc catttatatg
     8401 aactaattac attagttggt tcaaaaccac ttgcccgcat atccttcatg tgcacatatt
     8461 ccatatcgcg atcgcttttt aattcgatgc ggacaattac tttgtctcca acatgcaatt
     8521 ccgttttttc atcaaccgca cgcaataccg gacccgaagc ggaattgact tccagaaaca
     8581 attgcttttt cagcttcagg tttgtttctg ctgaagttac tttgtccatt tcttcaaaat
     8641 attgccaata catcgcccca taactcaata aattatttgc aggtggcgta atagtaatat
     8701 ttcccatggc aggattaata ttcgtgccca accatgttga tttaaaataa cccgttccca
     8761 attccgtctg ctccggtttt acttgcaggt tgccaatggt aatatttgcc agctcggtat
     8821 ttgccaataa atttagtcct ctcgacaaca aggcataaca agcttcggca gtggcgcggg
     8881 tcgttttcca gtctgttgtt tgtttctggc gcaataacca gatttgcaaa gcatccactg
     8941 catttttatc attcgccact tcagcaaaca cttcaatcat taaggcttgt gtttcgatag
     9001 gtgcctgata ccaataatat cccgcaatat tcgatttcca atacatcccc atctcctccg
     9061 atttaatact gttttctttc aacgaagcca caatttgctg cgctgtttta acctggtcgt
     9121 tgcgattcaa tattatggca atcatgcctt tctgataaag cgaataactt gtccaataag
     9181 tttttatctg agcaataaaa tatgcttttg cttcagcctc atttttagtg agtggaaatc
     9241 ttttttcatc aaaataactt gccgcatata aatattgtaa tatcaggtta tcaggaacat
     9301 aagaggattt ctcgaccttg tttttctcaa gtctggtata tgtctcatac atgtttgcag
     9361 aacaataagg catagcagat cgcaacatag catctacctc gccggcagta ttatattctg
     9421 ttgccgaaag tcgctccaga tgcgcgagtc ctgttacaat atattgcgtg atatatgggt
     9481 catctttata tccgctaaac caaggccagg cgccatccgg cagttgcatt ttttgcaatt
     9541 tttgcaaggc agcccgttgc tccgactgca ttttatttac atcaaaaagc agtgctacac
     9601 gttttttcct ttcagtttca ttttgcgcct gcaacaccca tggcgtttct tcaagcatca
     9661 gtgattttaa ttcctcattt ttttccagat tggaaaccaa tgagcttgga tttttcttcc
     9721 agctatcgaa aacacgtttg atttcagggt cactgttcac aatatgcgcc gcaatactat
     9781 tggcataaaa gcggctgaat acctgctcgg cacattcgta tggatattcc atcatataag
     9841 gcaacgcctg cactgcatac caggccggat ttgaggtata ttccaatgtc acgttttgat
     9901 tgcgaagtgt actgctgtta cctgatttca acaacttgtc gaatacaaat gatttgctat
     9961 cgccaccgcg cacccataaa ggaatagatt cggtaaccag aattttgttt gtcaataccg
    10021 gcaaaccatt ttcttcccca tcactaaacg tctcgtttcc ggctttaacg cgatatacga
    10081 tggcgtctat tccttcagga acatgcactt cccattgaac agatgtgctg ccttttgccg
    10141 atgcggtgaa cgataccgtt gcttgtttat tttcaaacac cgcgttcacc ggttgcatgt
    10201 taacggcatt aaatagttcc aattgcgcgg aaccgctgat gtctttatcg gtgagatttg
    10261 aaatcttggc agcaagcgta atgtgatctc cttcgcgtaa aaatcttggc atattcgcct
    10321 gtatcatcaa ttccttttgt gtgatcaccg atgccatggc agaacctgtt tgcaagtctt
    10381 tcgtatgtgc atatgccatg aacttccatg ttgtaagcgc atccggcatg gtgaatgaaa
    10441 agctgacatt tcccaggctg tcggtctcca gttgaggaaa gaaaaaagct gtttcattaa
    10501 aattattgcg catcggaatt tttcctagcg gcgcaggaac ttctcgttgg tttttctctt
    10561 ttaccacttc taaatcagca taaaaaccat tgcccacagc gcctttattt gttttagtaa
    10621 tttgcacttc ctgcatcata gcaggcatta aattatctaa cccttccccg gtaacaatgg
    10681 atcttgacct ttggtcaaca aaatcggcag cagctcctat atcataattc aaaaattgat
    10741 agccattcaa agaaaatcca aaccaattca attgattgta ttctattgta ggataagtgg
    10801 cgt
//
LOCUS       DAOQOU010000110        10721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3206_length_10721_cov_4.836396, whole genome shotgun sequence.
ACCESSION   DAOQOU010000110 DAOQOU010000000
VERSION     DAOQOU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_10721_cov_4.836396"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLM90_09915"
     CDS             <1..337
                     /locus_tag="PLM90_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ89733.1"
                     /translation="SGNVSDLVTSENISYAVVTFKDESIEKVYGKSTDTLGKFSIIIP
                     AGSYTFKVFFVGYAEINLDLTIRTGEMREINTGLGQDEAFVTYEINSPKKLNRRQLRE
                     EANKLNIKE"
     gene            449..943
                     /locus_tag="PLM90_09920"
     CDS             449..943
                     /locus_tag="PLM90_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HMZ89734.1"
                     /translation="MKAPTLLLLTTSLLFSACANKKDIIYVKPTDVTGLSVNSKVTVD
                     GYKVGEVDDMYLDNNGKVIVEIAFDEPVQIPTDSKFWISYVDLPDEKGITIDYGENKE
                     MLSPGDTVLAINKMAIFQSDSLAKMIQGILENISGAKQRDSILQELDRLNQNIDEIKD
                     LNAK"
     gene            1158..1595
                     /locus_tag="PLM90_09925"
     CDS             1158..1595
                     /locus_tag="PLM90_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89735.1"
                     /translation="MFSQDFKEFVELLINHKVDYLIVGGYAVGFHGHPRYTGDLDIWL
                     NPTSDNADRILACVNEFGFASFGLKRDDFTKEGNIIQLGYPPLRIDLLTQIDGVTFSE
                     CYQNKIQVEIDGLNVNFIGYNDLLKNKKESGRLRDLDDIENLT"
     gene            2050..2406
                     /locus_tag="PLM90_09930"
     CDS             2050..2406
                     /locus_tag="PLM90_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ89736.1"
                     /translation="MTIKVIKTEKEYQKALKRLEVIFDAKKASKDGDELELLSLLIDN
                     YEKEKYPIDLPDPIEAIKFRMEQLGYKQKDLAEAIGLKSRASEISNRKGKLTLEMIRK
                     LHDMLGIPTDVLVKDY"
     gene            2631..3077
                     /locus_tag="PLM90_09935"
     CDS             2631..3077
                     /locus_tag="PLM90_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ89737.1"
                     /translation="MTFREAIPADIPQIQIVRNAVKENTLSDPGLVSDADCLEFITKR
                     GKGWVCEIDNCIAGFAIVDMQDHNIWALFVDPAHEKQGIGRTLHDTMLDWYFWQTKTT
                     VWLGTAYNTRAEGFYRKAGWKEVGTHGPKEIKFEMRYEDWYANKKS"
     gene            3256..3597
                     /locus_tag="PLM90_09940"
     CDS             3256..3597
                     /locus_tag="PLM90_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ89738.1"
                     /translation="MSAHPLRRIVIYGNEFWDFYNKLPEKVKLRVDWTLDLVRSVEVI
                     PEKYFKHIESTDLYEVRVISGSNIYRIFCFFDKGKLIVLINGFQKKTQKTPKNEIERA
                     LRLKKQYYDQK"
     gene            3623..3880
                     /locus_tag="PLM90_09945"
     CDS             3623..3880
                     /locus_tag="PLM90_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ89739.1"
                     /translation="MDKRYGKKGTKTRDKYEQGFEAFKLGAILQEMRKEKGLTQEELA
                     LKCGTTKNYISRIENDASDIRLSTLMRIFSDGFGKQLRINF"
     gene            complement(4041..4337)
                     /locus_tag="PLM90_09950"
     CDS             complement(4041..4337)
                     /locus_tag="PLM90_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89740.1"
                     /translation="MRNFLVIIYYTYFVCVILDPFKDDAPLTIDPDTVIPFKPALQFL
                     KPVGWRYKQVINILSIMYHPQFSTRNILNLIWQPLRPSAMPNMLGFLIAKGFYH"
     gene            4336..4635
                     /locus_tag="PLM90_09955"
     CDS             4336..4635
                     /locus_tag="PLM90_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ89741.1"
                     /translation="MKKLKNIHPGEILLEEFLIPMEISAYRLAKETFLPQTRISEITK
                     GKRRITADTAIRLSKFFGTSAKFWLGLQDDYDLEEEKNQKDREFNNIKPMEGNAA"
     gene            4655..5044
                     /locus_tag="PLM90_09960"
     CDS             4655..5044
                     /locus_tag="PLM90_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89742.1"
                     /translation="MSFLSKLFGRRKQHTSSTQESFDEQYTAIGVFDYTEDGFVISDE
                     KRSIPIAWAEITKINVFKKDLVIYDLVMMEIVCGVSALVINEESPGWYQFVIKSKEVF
                     PGIPKHWDVEIIKPPFAENFSTIYERT"
     gene            complement(5064..5378)
                     /locus_tag="PLM90_09965"
     CDS             complement(5064..5378)
                     /locus_tag="PLM90_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HMZ89743.1"
                     /translation="MDNLTQMAEFMVDIDMHEAHSEAFMRLIPSQRAVVNDLMYEGKI
                     TSYCVSMDRKKLWMVAVARDEDEVMEFLAKFPLIKFMDCEIQPLLFSNSAQQTFSHIS
                     LN"
     gene            complement(5448..5933)
                     /locus_tag="PLM90_09970"
     CDS             complement(5448..5933)
                     /locus_tag="PLM90_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ89744.1"
                     /translation="MKTLYIIRHAKSDWDDKALSDHDRPLSSRGLRDAPVMASRFLQR
                     SRPPEFVLSSTALRALTTCKIFCDAWQIAHGNIHINKEFYFGSMPAMIRSIEEALAMH
                     DTVAVFGHNPNSSILANYWCSDFHHDIPTCAIVAIRFEKLVGADLGTLLWYDFPKNSV
                     K"
     gene            complement(5930..7615)
                     /locus_tag="PLM90_09975"
     CDS             complement(5930..7615)
                     /locus_tag="PLM90_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ89745.1"
                     /translation="MKNLRAYVVIALMGSVLFIPFLGASHLFDWDEINFAEASREMIV
                     TGNYTQTQIDFHQFWEKPPFFFWLQSASMHIFGVNEFAARFPNAICGILTLMILYAIG
                     KRLRDERLAGWWVICYAGSMLPQFYFKSGIIDPWFNLFIFLGVYGWIRYVQDNKQLKW
                     IVFSGIFAGLSMLTKGPVGVGLIGISVGIWYLFTRFRKFPNLWHILVFLICVAAPMTP
                     WVAIEISHNGTWFIKEFFTYQTRLATTEDASHGGFLLYHFVVILFLCFPASAFAIGNI
                     IKKNNVEQQHADFRKWMIILLSVVLVVFTIVQTKIAHYSSMAYFPLTFLAAQRLANID
                     KRWSSVEIVLIAVTGFVFSVSLFAIPFVLQRPELLETYILDPFSKEALRADAHWQGWE
                     PVMGMLMLGCCLFTVIWAMRSRGSAAITWLFLSSMVIINISINTITPHIERYSQGAMI
                     DFLDEKSREDVYTDVIGLKSYAQLFYGKRTPEDVNNPRFKAWAARTYPDVAEFDAIKW
                     RSAYGKWLREGDIDKPVWMVTNARKQQLFIGMPGFEPRGNKNGFYFFYRAPQP"
     gene            7672..9822
                     /gene="rnr"
                     /locus_tag="PLM90_09980"
     CDS             7672..9822
                     /gene="rnr"
                     /locus_tag="PLM90_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMZ89746.1"
                     /translation="MSKKKKKKSPKTQRAGLHFEDELFDFISSKLAKGASLKVIFKWA
                     SKTGEMDGVFDAIENLVNAGKIEEFAPDLFRSKQQKGGGATMVGIVDMTQSGNAYVVI
                     DGHLYDVFVPSHKTQRAFNGDTVRVRITSMGKNKKPEGEITEIIKREKELFVGTVDIN
                     GQHSFAVTDDRFNNYDFYLPAEEVKKHKLTHGDRIIMRIRDWPPGMKSPIGQVVTKLG
                     APGDHASDMEAILVETGIIYAFPKEVEKEADAIPVTIPEKVIASRLDIRDRFTITIDP
                     YDAKDFDDAISYRKLDNGNLEIGVHIADVSYYVTSGSAIDKEAYARGTSVYLVDRVLP
                     MLPEKLSNNVCSLRPHEDKLCFSAIFEMDEEGNLIEEWFGKTVIHSDRRFTYEEVQEV
                     IETGKGDHVEEIMILNKVTHALRKKKFAAGAISFETQEVKFILDENFVPTGLYVKERK
                     EAHMLIEDLMLLANRRVATFLAKEHSRVPFVYRVHDRPDMQRLEEFSMNARRFGYKLK
                     LDSPKNISAELNRLMVEIKGKPEQNILENLAIRCMAKAVYTTKNIGHYGLAFDYYTHF
                     TSPIRRYPDLMVHRVLEDVLDKSPLTYPKIDDLEDHCRHCSATERKAMEAERESVKYK
                     QVEYLSKHVGEEFDGVISGITPYGIFVELIANKCEGMVHVDEIRDELMFDESRRRLVS
                     LTHSKDYELGDNIRIKVKKADLAHRRLDFTPVIQ"
     gene            complement(9803..10471)
                     /locus_tag="PLM90_09985"
     CDS             complement(9803..10471)
                     /locus_tag="PLM90_09985"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ89747.1"
                     /translation="MQPTIIFEDSQLIVLNKPAGIAVEADRFGHPGVQGWVASYFDGK
                     KMPSNTIIGIVHRIDRPVSGVVLMAKKKSVLVDLNAQFAAGTVSKVYYAGVEGTPPKP
                     EGKLEHFLMKDPEQKKAIITPKKSKRSAAVSLSYKTLHSDKGITLLEVRPHTGKYHQI
                     RAQLSEMGCPIIGDALYGSKKSFLQDAILLHAASLTCMHPATKEEVTFSAPLPNAWPI
                     VLPV"
BASE COUNT         3493 a         2119 c         2202 g         2907 t
ORIGIN      
        1 atcaggcaat gtttctgatt tggttacttc agaaaacatt tcatatgctg tagttacgtt
       61 caaagatgaa agcatcgaaa aggtctacgg gaagtcaacc gacactctcg gcaaattttc
      121 aattattatt cctgcaggaa gttatacttt taaagtattc tttgttggtt atgctgaaat
      181 caatttagat ttgacaataa gaacaggcga aatgcgggag ataaacaccg ggttagggca
      241 agatgaggcc tttgtcacct atgaaatcaa cagcccgaag aaactcaata gacgacagct
      301 gagagaggaa gccaataaat tgaatataaa agagtaagcc tcgaaattat gattgcaaaa
      361 tttaataatc cttcaaaaaa cgagtaattt tcaattcaga tcaactagtt actgcaaagc
      421 aaactcgtaa ttttccttta caaatatcat gaaagcccct acccttctgc ttcttaccac
      481 ttccctgctt ttcagcgcct gtgcaaacaa aaaggatatc atttatgtaa aaccgaccga
      541 cgttaccggc ttatcggtaa actctaaggt tacagtcgat ggttacaagg ttggcgaggt
      601 cgacgatatg tatctggata ataacggaaa agttattgtc gaaattgctt ttgatgaacc
      661 ggttcaaatc cctacagatt caaaattttg gataagttat gtggatttgc cagatgaaaa
      721 aggtattaca attgactacg gcgaaaacaa agaaatgcta tccccgggtg atacggttct
      781 agcaataaat aagatggcaa ttttccaatc agattcatta gctaaaatga ttcaaggtat
      841 ccttgaaaat atttccggcg ccaaacaaag agactcaata ttacaagaat tggaccgcct
      901 gaatcaaaat attgatgaga taaaagattt aaatgcaaaa tagtcgcact acaaggcact
      961 tcaactaaat aaattaaatt gattgcaatt ttattttatc aacaaaattg cctatatttg
     1021 aatcatggaa aaggttgtaa aaattaccac cctgcaagac caaaaatccg actttgcata
     1081 ttggtcgact cggagcataa cagaacgttt agacactata gaaattctta gacaacaata
     1141 ttttaactta aaaagtgatg ttcagccaag acttcaaaga gtttgtagaa ttattaataa
     1201 atcataaggt agattacctt attgtcggtg gttatgccgt tggtttccac ggccatccgc
     1261 gctacacagg agacttagac atttggctta atccaacatc ggacaacgca gacagaattt
     1321 tagcctgtgt gaatgaattt ggtttcgcct catttggatt aaaacgcgac gactttacaa
     1381 aagaaggtaa tatcattcaa ttagggtatc caccactgag aattgattta ttgactcaaa
     1441 tagacggggt gacctttagt gaatgttatc agaacaaaat tcaggttgaa attgatggtc
     1501 ttaatgtaaa ttttattggg tataacgacc tattgaaaaa caaaaaagag tccggtcgac
     1561 tcagagatct tgacgacatc gaaaatctca cttaagcctt tgggttcact gccgggatag
     1621 tttctttaac aacaaaacca atcaccggta gaaatattgt ttacggctgt tatgtccagt
     1681 ccagttttgg aaatttttta tttgttcgta gagtcccatt tagggaacct ttaaaattat
     1741 gtattgcgcg agttactgca aaaagcattt gatggacctt tcggatcagg cacatggacc
     1801 ttggcttcga agaatcgtat aatcatggta taaagaggct gaagaagccg gctggaaggg
     1861 tccgagaggc ataaagcgcg acttaccaac agccggtttt ctagccgata accgggtagt
     1921 ttttaatatc aaggggaata attaccgcct ggttgttagg atcaactacg catatgcaat
     1981 tgtttggatt cggtttgtcg gcactcatgg tcaatatgat aaaataaatg catcaaatat
     2041 ttaacttata tgacaattaa agtaatcaaa acagagaagg aatatcaaaa agcacttaag
     2101 cgcttagaag tgatctttga tgctaagaaa gcctcaaaag atggtgatga attggaattg
     2161 ctttcattac tcatcgacaa ctatgaaaaa gaaaaatatc ccattgacct gcctgacccg
     2221 atagaagcaa ttaaattccg aatggagcaa ctcggataca aacaaaaaga ccttgccgag
     2281 gcaattggat taaaaagccg tgcgagtgaa atttcaaatc gcaaaggaaa attaacactt
     2341 gaaatgattc gcaaattgca cgacatgtta ggtataccaa ctgatgtgct ggtgaaagat
     2401 tactaaaatc acatatacat ctcaagtaga tcccaccagg gtagattgtt accaataata
     2461 aaagagtttt ccaacgtgga tacagggaca aacactcctt tcatcagctg cataatatcc
     2521 cactgtagct tcaattaaat accttccatt aaaaaacatt cttaatttta tcgggattgt
     2581 caggccacaa aaactgaaat ttgcctattg catcataact atcgatcacc atgaccttca
     2641 gagaagccat acccgctgac atcccgcaga tacagatcgt gcggaatgcc gttaaggaga
     2701 ataccctctc ggatcccggc ttggtaagcg atgccgactg ccttgaattt atcactaaaa
     2761 gaggcaaagg ctgggtctgt gagatcgaca attgcattgc aggcttcgcc atcgtagata
     2821 tgcaagacca taatatctgg gcattatttg ttgatcctgc tcatgaaaag caaggcatag
     2881 gaagaacact ccatgatacc atgctcgact ggtatttctg gcaaaccaaa acaaccgttt
     2941 ggctgggaac agcctataat acgcgggctg aaggctttta tcgcaaagcc ggatggaaag
     3001 aagtaggaac gcatggtcct aaagaaatta agtttgaaat gcgttatgag gattggtatg
     3061 ctaataaaaa gtcgtaacac aatgctgatt ttaaggggaa tgcgatgttc cgtcaacaat
     3121 acatgggcga atttagattg aataggatcg ctttgaaccg aaatacaggc cacacagctc
     3181 aatgaataaa attatcactc aggcggcttt ggggtgacat ttggaaaatg ttacctaatt
     3241 aggtaacttt gcgaggtgag cgctcatccc ctaagacgaa tagttattta tggaaatgaa
     3301 ttttgggact tctacaacaa attacctgaa aaagtaaagc tacgtgtcga ctggacacta
     3361 gaccttgtca gatccgttga agtaattccc gaaaagtatt tcaaacatat tgaatcgacc
     3421 gatttgtatg aagtacgtgt tatttccgga agcaatattt ataggatctt ttgcttcttc
     3481 gacaaaggga aactaattgt gcttataaat ggctttcaaa agaagacaca aaaaacacca
     3541 aaaaatgaaa ttgaaagagc acttcgactt aaaaaacaat attatgacca gaaataaaaa
     3601 aatcacaaac tttgatgtcc acttggacaa aaggtatgga aaaaagggaa caaaaacacg
     3661 tgacaagtat gaacaagggt ttgaggcttt caagcttggc gcaattttac aggaaatgcg
     3721 caaggagaaa ggacttacac aggaggaact tgccttaaaa tgcggaacaa ctaaaaatta
     3781 tatctcgaga atagagaatg atgcatcaga cattagactg tcaaccttga tgcgcatttt
     3841 ttctgatgga ttcgggaagc aattacgcat taacttctag aattcacgtc aaaccaattt
     3901 ttaggccgaa ttcttgcaaa acaacataat aaatacgcca cttaccaacc ctcgaattcc
     3961 acgtacaccc gttcaaagct tcaaaacttg tatgatacca aaaaattact tacttttgac
     4021 gttagtaacg caagacataa ctaatgataa aaacctttgg cgataaggaa accgagcata
     4081 tttggcatgg cactcggtcg aagcggttgc caaatgagat tcaagatatt gcgcgtagaa
     4141 aattgaggat gatacataat gctcaagata ttaatgactt gcttgtaccg ccagccaaca
     4201 ggcttgagaa actgaagcgc aggcttaaag ggtatcacag tatccggatc aatggtcaat
     4261 ggcgcatcat ctttaaatgg gtcaagaatg acgcatacga agtaagtata atagattatc
     4321 actaaaaaat tacgcatgaa aaagctgaag aacattcatc ccggtgagat cctattagaa
     4381 gaatttctga ttcctatgga aatttctgcc tatcgtttag caaaagaaac cttccttcca
     4441 caaacgcgca tttcagaaat aacgaaagga aaaagaagga tcacagctga tacagcaatc
     4501 agattatcca agttcttcgg aacaagcgcc aagttctggc tggggcttca agatgattat
     4561 gatctggaag aggaaaaaaa ccaaaaggat cgagagttta ataatatcaa gccaatggaa
     4621 gggaacgctg cataatcatt ttaatcttca cctcatgtcc ttcctctcca aactattcgg
     4681 cagaagaaaa cagcacacat caagtacgca ggagtcattc gatgagcaat atactgccat
     4741 tggtgtgttt gattataccg aagatggttt tgtcatcagc gatgaaaagc gttcaatacc
     4801 aatagcatgg gctgaaatca ccaagatcaa tgtgtttaaa aaagatctgg taatatatga
     4861 tctggtgatg atggaaattg tatgtggtgt gtctgcattg gtcatcaatg aagaatctcc
     4921 aggctggtat caatttgtaa tcaaatcaaa agaagtgttt ccaggcattc caaaacattg
     4981 ggatgtcgaa atcataaaac ctccatttgc agaaaatttt tctactatct acgagcgcac
     5041 ttaatactat accttgattg atttcaattc agcgaaatat gcgagaaagt ctgctgtgct
     5101 gaattactga aaaggagcgg ttgtatttca caatccataa actttataag tgggaatttt
     5161 gcgaggaact ccattacttc atcctcatcc cgggctactg ctaccatcca cagttttttc
     5221 ctgtccatag atacacaata gctggtgatc ttaccctcat acataagatc attcacaacg
     5281 gcgcgttgcg agggaatcaa acgcataaat gcctctgaat gagcttcatg catatcgata
     5341 tcaaccataa attcagccat ttgtgtcaga ttgtccatac aaacttacaa atttttaata
     5401 tgaacatata aacgacaaaa accgttcccc ggttgtatgt aaaccgttta tttaacagaa
     5461 ttctttggga aatcatacca caacaaagtg ccaagatccg ctcccaccaa cttttcgaag
     5521 cgtattgcaa ctatggcaca agtgggaata tcgtgatgga agtcgctaca ccagtagttc
     5581 gccaaaattg aagaattggg gttatgtccg aaaacggcaa cggtatcatg catcgccagc
     5641 gcttcctcaa tactgcgaat cattgccggc atagacccaa agtaaaactc cttattgata
     5701 tgtatatttc catgcgctat ttgccaggca tcgcagaaaa ttttgcaggt ggttaatgct
     5761 ctgagtgctg tgctactcaa cacaaattca ggtggtctgc ttcgctgtaa aaatcttgat
     5821 gccatcaccg gtgcatcgcg caaacctcgt gacgacaaag gtctgtcatg atcggataat
     5881 gctttatcat cccaatcgct tttagcgtgt ctgatgatat ataaggtttt catggttgag
     5941 gggcgcgata aaagaaataa aatccatttt tatttccacg tggttcaaat ccgggcatgc
     6001 cgataaataa ttgttgcttt ctggcattgg taaccatcca cacaggctta tcaatatctc
     6061 cttcgcgcag ccatttaccg tacgcactac gccattttat agcatcaaat tccgctacat
     6121 caggataagt gcgtgcagcc caggctttaa atcgcggatt atttacatct tccggcgtgc
     6181 gcttaccgta gaataattgc gcataacttt ttaatcctat cacgtcagtg taaacgtctt
     6241 ccctactctt ctcatcaaga aaatctatca ttgcaccttg cgaataacgt tctatatgcg
     6301 gggtgatggt gttaatactg atatttataa tcaccatact gcttaaaaat agccatgtaa
     6361 ttgcagcact tccgcgtgaa cgcatcgccc agattacagt gaataaacaa catccgagca
     6421 tgagcatacc cattaccggc tcccaaccct gccaatgagc atctgctcga agggcctctt
     6481 tagaaaaggg atccaaaata tatgtctcta ataattcagg tcgttgcaat acaaatggaa
     6541 tagcaaataa tgatacactg aaaacaaatc ctgttactgc gattagaaca atctccacgc
     6601 tgctccatcg cttatctata tttgccaatc gctgagccgc aagaaatgta agcgggaaat
     6661 aagccataga agaataatgt gcaattttag tctgcacgat ggtgaataca acaagcacta
     6721 cacttaataa aataatcatc cattttctaa aatcggcatg ctgttgttca acattattct
     6781 tcttaataat atttccaatt gcaaatgcac ttgccgggaa acacagaaat aagattacca
     6841 caaagtgata caataaaaat cctccgtgac tggcatcttc agtagttgcg agtcgcgttt
     6901 gataggtaaa aaattctttg ataaaccagg ttccgttatg cgaaatttct atcgccaccc
     6961 aaggtgtcat cggcgcagcc acacatatta aaaatacaag aatatgccag agattcggga
     7021 attttctgaa tcgagtaaaa agataccata taccaactga gataccaatc aacccgacgc
     7081 cgacaggtcc ttttgtgagc atcgataagc ctgcaaatat tccgctgaat actatccatt
     7141 ttaattgctt gttgtcctgc acatatctta tccacccata cacaccgagg aaaatgaata
     7201 aattaaacca ggggtcgatg atgcctgatt taaaataaaa ctgcggcagc atacttcccg
     7261 cgtaacaaat cacccaccat cccgccaatc gttcatcgcg caggcgcttg ccgattgcat
     7321 acaaaatcat caaggtgaga ataccgcaaa tggcattggg aaatcgcgct gcgaattcgt
     7381 taacgccaaa tatgtgcatg gatgccgact gcaaccagaa gaaaaatggc ggtttttccc
     7441 aaaattgatg aaaatcgatt tgtgtctgcg tgtaattgcc ggtcactatc atttcacgtg
     7501 aggcctcggc gaagttgatt tcatcccaat caaacagatg cgaagctccc agaaaaggga
     7561 tgaacaaaac actacccata agggctatca ccacataagc acgcaggttc ttcatattca
     7621 ggcggtggaa aattacaaaa aggttatcgt tcatacccgt atctttaggg gatgtcgaag
     7681 aaaaagaaga agaaaagtcc caaaacgcag cgtgccggac tacattttga agatgagctt
     7741 tttgatttca tctccagtaa gttagccaaa ggtgcctcgc tgaaagtgat attcaaatgg
     7801 gcctctaaaa ccggggaaat ggatggtgtg ttcgacgcaa tagagaattt ggtgaacgcc
     7861 ggaaaaattg aagagtttgc acctgacctc tttcgctcca aacaacaaaa aggtggcggc
     7921 gctaccatgg tgggtatcgt agatatgacg caaagcggaa atgcgtatgt ggtgatcgat
     7981 ggacatttat atgatgtatt tgttccttcc cataaaacac agcgcgcatt taatggcgac
     8041 actgttcgcg ttcgtatcac ctcgatggga aaaaataaaa aacccgaagg tgaaataaca
     8101 gaaataatta aacgagaaaa agaattattt gtaggcacgg tagatatcaa tggtcagcat
     8161 tcatttgctg ttactgacga cagatttaat aattatgatt tttatcttcc tgccgaagaa
     8221 gtaaaaaaac ataaactcac ccatggcgat cgcattatta tgcgcattag agactggcct
     8281 ccgggaatga aaagtcctat cggacaagta gtaaccaaac taggcgctcc cggcgatcat
     8341 gcgagcgata tggaagcgat tttggttgaa acaggaatta tttatgcttt tccaaaagaa
     8401 gttgaaaaag aagctgacgc tataccggtg acaattcctg aaaaagtgat cgcctctcgc
     8461 ctcgacattc gcgatcgttt tacaattact atagatccct atgatgcgaa agattttgat
     8521 gacgccattt cctatcgcaa gctcgataat ggcaatttag aaatcggtgt acatattgca
     8581 gatgtttcct attatgtgac ttcaggctca gccatcgata aagaagcata cgcaagaggc
     8641 acatcggtat accttgtaga tcgtgtgttg ccgatgttgc ctgagaaatt gtcgaataat
     8701 gtatgctctc tgcgcccgca tgaagataaa ttatgcttca gcgcaatttt cgaaatggat
     8761 gaggagggaa atttaattga agaatggttt ggcaaaaccg taatccatag tgatagaaga
     8821 tttacttatg aagaggttca ggaagtcatt gaaaccggca aaggtgatca tgttgaagaa
     8881 ataatgatcc tgaataaggt gacgcatgca ttgcgcaaaa agaaattcgc tgcaggtgcc
     8941 atatcattcg aaactcagga ggtcaaattt attttagatg aaaattttgt gccaacggga
     9001 ttatatgtaa aggagcgaaa agaggcacac atgctcattg aagatttgat gctgctcgcc
     9061 aatcgtcgtg ttgccacctt ccttgcaaaa gaacacagtc gcgtgccttt tgtttatcgc
     9121 gttcatgatc gtcccgatat gcagcgatta gaagaatttt cgatgaatgc ccgtcggttc
     9181 ggttataaac tgaaattaga ttcgccgaaa aatatttctg ctgagttaaa cagactaatg
     9241 gtagaaataa aaggtaaacc ggagcaaaat attctcgaga atctcgccat ccgatgcatg
     9301 gcaaaggcgg tgtataccac caaaaatatc ggtcactacg gacttgcatt tgattattat
     9361 acgcatttca cctctcctat tcgtcgctac cccgatttaa tggtgcacag ggtgttggag
     9421 gatgtgttgg ataaatcgcc actcacatat ccgaaaatcg acgacttgga agatcactgt
     9481 cgccattgtt cagccaccga acgcaaggcc atggaagcag agagagaaag tgtgaaatac
     9541 aaacaagtag aatacttaag taagcatgtt ggcgaagaat ttgatggcgt tatcagcggc
     9601 atcacacctt atggaatttt cgttgaactg attgccaata aatgcgaggg catggtacat
     9661 gttgatgaga tcagagatga actcatgttt gatgaatctc gccggcgatt agtgagtctt
     9721 acccattcaa aagattatga gcttggcgat aatattcgca tcaaagtgaa aaaagctgat
     9781 ctcgcacatc gcagattgga ttttacaccg gtaatacaat aggccaggca ttcggaagtg
     9841 gcgcagaaaa agtcacttct tcttttgttg ccggatgcat acaagttagc gaagcggcgt
     9901 gcaacaatat tgcatcctgc aaaaatgatt tctttgaacc atacagtgca tctccaataa
     9961 tgggacaacc catttcactt aactgtgcac gtatttgatg atattttccg gtatgtggtc
    10021 gcacttcgag gagtgtaatg cctttgtcgg aatgcaatgt tttatatgac aaagaaactg
    10081 ctgcactgcg cttacttttt ttgggggtga tgatggcttt tttctgttcg ggatctttca
    10141 ttaaaaaatg ttcaagcttg ccctcaggtt ttggaggtgt accttccact ccggcataat
    10201 acactttaga caccgtgcct gcagcaaatt gcgcattaag atccaccagc accgattttt
    10261 tcttcgccat cagtacaaca ccgctaacgg gtctgtctat gcgatgcaca ataccgatga
    10321 tggtgttgga tggcattttc tttccatcaa aatatgatgc cacccagccc tgaacgcccg
    10381 gatgcccgaa acgatcggct tcaacggcaa ttcccgcggg tttgtttaat acgattaatt
    10441 gcgaatcttc aaatatgata gtcggctgca tggggcaaag gtggtgaata atttgagaat
    10501 ttgagaatta gctgattaac aaatgggatg gtgcatgcat ggagattttt atcatagtta
    10561 gcaagtttga gggttagcaa gttagcaagt gaggttaatt agcaaatttg ggaattagca
    10621 aattagcaag ttaggaagtt agcaagtgtg atgggctttt aacgctggcg cgttaatagg
    10681 aaatgcaatt atattgcttt gcaatatttt aattccgaag c
//
LOCUS       DAOQOU010000111        10664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3229_length_10664_cov_5.332265, whole genome shotgun sequence.
ACCESSION   DAOQOU010000111 DAOQOU010000000
VERSION     DAOQOU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_10664_cov_5.332265"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLM90_09990"
     CDS             <1..505
                     /locus_tag="PLM90_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89748.1"
                     /translation="RTNDGIIVAATHGRGIWQATLDHHGLLIDPFWSERGPANVGGRT
                     RTIMVDPNDPTGKTVWAGSIAGGLWKTTDIDAVPVYNQTASTFTLSVSPNPASNIIRI
                     NLSGTSSKGATLTMYRMDGSVAAVIGKSQLSGQVSWQIPSGLSSGTYLLVATDGQNRA
                     VQRVVVL"
     gene            576..1550
                     /locus_tag="PLM90_09995"
     CDS             576..1550
                     /locus_tag="PLM90_09995"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HMZ89749.1"
                     /translation="MREIRFREALREAMVEEMRKDQHILLMGEEVAEYNGAYKVSQGM
                     LDEFGAKRVIDTPIAELGFAGIGVGAAMNGLRPIVEFMTFNFALLAIDQVVNSAAKML
                     AMSAGQFRCPIVFRGPSGSAGQLGAQHSQMFENWYANVPGLKVISPSNPYDAKGLLKS
                     AIIDDDPVIFMESELMYSDMGAVPEEEYYIPIGKADVKRSGSDITIVSFNKMMKVALK
                     AAEELEKDGISAEVIDLRTIRPLDINTIVESVKKTNRIVVVDESWPYGSIASEIAYQL
                     QKDAFDYLDAPVRRVNGADTSMHYAPNLVDAYLPNPKKVIEVVKEIVY"
     gene            complement(1774..3219)
                     /locus_tag="PLM90_10000"
     CDS             complement(1774..3219)
                     /locus_tag="PLM90_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ89750.1"
                     /translation="MRAKILGMTMMVAVISSLATVVVYDRFIADYGHNENGSNSPYAF
                     TKASFPVAPSGMAPVDFTYSAALTTPTVVHVKTTFKQQANYGGSNDIIRQFFGDDFNW
                     EQPSAQASGSGVIMSEDGYIVTNYHVVKNADDIEVVFFDKHSAKAELIATDPSTDIAL
                     LKVDLDGLPAIRFGDSDSVRVGDWVMAVGNPFDLASTVTAGIVSAKGRKIDILGENAS
                     TPVESFIQTDAAVNPGNSGGALVNLQGELIGINTAIATPTGTFAGYSFAVPVNIVKKV
                     VKDLKAYGVVQRAYLGVEVDMTREDVGGVYVSNVVKGSGADESGIKEGDVITHINSSA
                     INSFPELQEQLSKYGPGEKVNVSLVRGNDKKNVVVLLKNQKGNTELIKKESNETLSSL
                     GIEAEEISAREAAYYQVDGGVKVTEIKNGKVFQQTRIREGFVITAIDDKKIVNYNDLA
                     KILNDKKGNNVIIEGFYPSFPNKIYNYGLSL"
     gene            3342..4127
                     /gene="dapF"
                     /locus_tag="PLM90_10005"
     CDS             3342..4127
                     /gene="dapF"
                     /locus_tag="PLM90_10005"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMZ89751.1"
                     /translation="MPIQFYKYQGTGNDFVLFDNRSGQIDRTRYDWFNFICDRRFGIG
                     ADGVMLLQEREGYDFEMLYFNADGLPGSMCGNGGRCMVQFAKRLGIDKAQYHFLASDG
                     DHDAIITGDEVSLHMRDVLSVELHKGQYILNTGSPHVVLAVDDVMAVDVNRDGAQLRY
                     DPLFAPGGTNVNYIHIQGETMHIRTYERGVERETLSCGTGVTASALVSALMQNLPFGT
                     HEVPIRTMGGALRVQFHYHADGSFTDIYLIGPGTFVFSGEINA"
     gene            4315..7059
                     /locus_tag="PLM90_10010"
     CDS             4315..7059
                     /locus_tag="PLM90_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HMZ89752.1"
                     /translation="MSIIKTPDQRVRVFISSTINELAEERIAARSAIESLKLIPVFFE
                     AGARPHPPRDLYSAYLSQSHIFLGIYWNSYGWIAPGAEISGLEDEYRLCGKTKPKLIY
                     VKNSTERQEKLDLLLQDISNSDSACYQKFNTADELKQLIENDLSVLMSEIFESSLISG
                     QGSIDSSTGAIENKHDHPIIDIPLIKSEMIGRERDLQLVSELLLREDTSLITILGAGG
                     TGKTTLATFIGQSNKDKFKDGVLFIQLAAITNSKLVVNTIGEKLGIQDNGKHDLISLI
                     IDLLTGKQFLLILDNFEQVVDASVVVSQLINQCKDLKVLVTSRSILHIRNERIYHLAT
                     LNFPVEESAIAFADLQNYAATKLFLDRAQEVNQNIENSKENAEAILEICQRMDGLPLA
                     IELAAARTRFFQPAALLGRITKTLDLVNKGHKDLPERQQTLRGAIEWSYNLLSPDTQS
                     IFRQMAVFKRSWTMEAADAILVINESAVDAEEYTERLLDVSLIKTDILNKELEPRFNM
                     LQTVHEYAGEMLAQDPDCEKVKLQYAKYFLQLCEDAEPYLWNRNGAIWLDRIESEYQN
                     IRASFYIFMELGLPEMAWRFFPCLAPYWTIRGGFTETVMLIKDAGLYEESSWTIGTID
                     PVVHGRALTWSGYCLLFVFDLERGFAMLQAAQNILTATNDIYNLTYAYCFDGCYGAYM
                     QKEDAEEKILKGFELLERQNNPLINCMFYCWSDEYFRQKGQPEIRDKNLNLAASIAKE
                     HGITYIQGAVMLIRATIATSEPEMDSDKLIQIGQEMFMIFPEKGYQGFKAASKQFQAI
                     GYLNKGDIIAARNMIKESAEFVKLSGELESEFYLCMLLVHILALGGDRKHAFSIFGAL
                     HTFIEQSQYPVIGGAAVQYQTISQLLQPYEAQPECQSWFESGKSMPLTSAVNMGLAQI
                     "
     gene            7193..8212
                     /locus_tag="PLM90_10015"
     CDS             7193..8212
                     /locus_tag="PLM90_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HMZ89753.1"
                     /translation="MLKKNHIFAHLKFSIRSRWKEMIKYYKKIDNQIVEIDRPEKDCW
                     INVYPPFDHKRLATLSDAIDIPIDYLLDSIDINERSRFEQDDNVKLIVINTPIENDME
                     NSIDNDAFYITIPIGIILTQDYNIIISSAQNKVIDWFFSIAIKHLSPHDKDLVVLKIF
                     EKNVFYFIQYLNEMNKRRYLIEKELMHSSRNTELAKLLNIQKSLVYFVTDLRANELLM
                     MKIQRTNFLGIKNDEEKNDYLTDIVIDSSQALEMATVYTNILNGTMDAFGSIISNNLN
                     SVMKRLTSITIILMVPTLIASFYGMNFANLPLENSNSGFYVIMLFSILISFALYWVFR
                     RIRWF"
     gene            complement(8239..9156)
                     /gene="fmt"
                     /locus_tag="PLM90_10020"
     CDS             complement(8239..9156)
                     /gene="fmt"
                     /locus_tag="PLM90_10020"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMZ89754.1"
                     /translation="MAHHLRIIYMGTPDFAVPGLQKLVEAGFNVVAVVTAPDKPSGRG
                     LQVKPTPVKVYADSQGIPVLQPVKLKDPEFISTLRTYNADLQVVVAFRMLPEVVWNMP
                     PKGTINLHASLLPDYRGAAPINHAIINGEKETGATTFFLQHAIDTGDLIDQIRIPIHD
                     DDNAGSIHDTLMMLGADLIVKTVIAIEENNYSTKPQSGESNKIAPKIFRENCRIDWNQ
                     PAERIHNVIRGLSPYPGAFTDIKGPQWKILKSKFEIEPHQQSPGTIATDAKTYLKIAS
                     ADGWVYVLAIQPEGKRNMDIATFLHGYRS"
     gene            complement(9156..10388)
                     /locus_tag="PLM90_10025"
     CDS             complement(9156..10388)
                     /locus_tag="PLM90_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HMZ89755.1"
                     /translation="MRMLLFNLMLAGLLNTAACKNSDSVTVSAIDTVHTDTHNDDAIL
                     TGAEQTELWMPMLKGKEVALVVNQTSVIGKKHLVDTLIAAGVHISTIFAPEHGFRGTE
                     DAGATILNAKDTATGIPVLSLYGNKKKPLPQDLADADIIIFDIQDIGARFYTYISTLH
                     YVMEACAENKKKLLILDRPNPNGYYVDGPVLEKAYTSFVGMHQIPIVHGMTIGEYARM
                     VNGEHWLTNGEQCELQVIPCANYDHTDIYNLDIPTSPNMRTMQAIYLYPTLCLFEGTN
                     VSVGRGTDKPFEYIGSPYLPPTGFSFVPKSGSGAKTPPFMGQTCNGYDLSKWPVDSVR
                     SGKINLAFFLEVYHNYASSTPFFLENNFFNKLAGNAELMQQIKAGLSEEEIRLSWQDD
                     ISAFKQVRKKYLLYPDFE"
     gene            10428..>10664
                     /locus_tag="PLM90_10030"
     CDS             10428..>10664
                     /locus_tag="PLM90_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89756.1"
                     /translation="MWFEYFVSNRIMHNKERSFSRLIMGIARVAIALSLSVMLISTSL
                     VNGFRATISEKVYGFWGHINIAKQSLRNSFDDIPI"
BASE COUNT         2936 a         2143 c         2466 g         3119 t
ORIGIN      
        1 gcgaacgaac gacggtatca tcgtagcggc aacacatggt cgcggtatct ggcaagcgac
       61 attggatcat catggtttgt tgatagatcc gttctggtcg gaacgcggac ctgcgaatgt
      121 tggtggcaga acgcgcacca ttatggtaga tccgaatgat ccgacgggaa aaacggtatg
      181 ggcaggaagt attgcgggtg gattgtggaa gacgacggat attgatgcgg ttcctgttta
      241 taatcaaacg gcatcgacat ttaccttgag tgtttccccg aatccagcga gcaatatcat
      301 ccgcataaac ctgagtggca cttcatctaa aggtgctacg ctgaccatgt atcgcatgga
      361 tggttcggtg gctgcggtga tagggaaatc gcaactttcg ggacaggtaa gctggcagat
      421 accttcgggt ttatcgtcgg gcacttatct gttggtggct acggatggcc aaaacagggc
      481 ggtacagcgg gttgtcgtgc tataagcctg tcgcggatcc ggactgccat gctgtgccga
      541 acatttccta attttgcagc aaatttcagc atacaatgag agagatccgc tttcgtgagg
      601 ctttgcgtga ggctatggta gaagagatgc gcaaagacca gcatatcctg ttgatgggtg
      661 aagaggttgc cgaatacaac ggtgcctata aagttagtca gggcatgtta gatgagtttg
      721 gtgccaagcg cgtgatagat acacctattg cagaacttgg atttgcaggt atcggcgttg
      781 gtgctgcgat gaatggattg cgtcctatcg tggaattcat gacattcaat tttgcattac
      841 tcgcaattga tcaggtggtg aactctgcag caaaaatgtt ggcgatgagt gcgggacagt
      901 ttcgttgtcc tattgtattt cgtggaccta gcggttctgc gggacaatta ggtgcgcaac
      961 actcgcagat gtttgaaaat tggtatgcga atgttccggg gttgaaggtg atatcgcctt
     1021 cgaatccgta tgatgcgaag ggattattaa aatctgccat cattgatgat gatccggtta
     1081 ttttcatgga gagtgaattg atgtattccg atatgggtgc agtgcccgag gaggaatatt
     1141 atattcctat cggtaaagcg gatgttaaac gcagcggaag tgatattacc attgtttcat
     1201 ttaataaaat gatgaaggtg gcattaaagg ctgctgagga attagaaaaa gacggcatca
     1261 gtgccgaggt aatagatctt cgtactattc gtccgctcga catcaacacc attgttgagt
     1321 cggtgaaaaa aacaaatcgc attgtggttg ttgatgagag ctggccttat ggaagcattg
     1381 catctgagat tgcttatcaa ttacaaaaag atgcatttga ttatttagat gcgcctgttc
     1441 gccgagtgaa tggtgcggat acgagcatgc attatgcacc gaatctggta gatgcttatt
     1501 tgccaaatcc gaagaaggtg attgaggtgg tgaaggagat tgtgtattga tttttttcac
     1561 gcagaggcgc agaggcgcag aggggtgagt gattactacg tcttgcacac ttccacactt
     1621 ccacactatc gaaggaagag cttcaggcaa cgtatcgttc cagttgacaa ataagccttg
     1681 tcgtaagtac aaaaaaaagc cccgctgtgc cggagaggcc ttggcggagc tcacatttga
     1741 aaacccaatt accctatatc ttcaattatt tctttataaa cttaacccgt aattatagat
     1801 tttgttcggg aatgatggat aaaatccttc aattataacg ttgtttccct ttttatcgtt
     1861 caaaatcttt gccaaatcgt tataatttac aattttttta tcatctattg ctgtaattac
     1921 gaatccttcg cggatgcgtg tttgttgaaa cactttgcca ttcttgattt cggtgacttt
     1981 tacgccgcca tcaacctgat aatatgctgc ttcgcgtgcg ctaatttcct cagcttcaat
     2041 gcctaagctt gataatgttt catttgattc ttttttgatc agctcagtgt tgcctttctg
     2101 attctttaat aagacgacaa cattcttctt atcgttgcca cgcacgaggc ttacatttac
     2161 tttttcaccg gggccatatt tactcagttg ttcctgcaat tcggggaatg agttgatagc
     2221 tgaactattg atatgtgtga ttacatcacc ttctttaatt ccgctttcat cggcacctga
     2281 tccttttacc acattgctta catacacacc gccaacatct tcgcgtgtca tatctacttc
     2341 aacacccaaa tatgcgcgtt gcactacacc gtaagctttg aggtctttca ctactttttt
     2401 aacaatattc accggcacgg caaaagaata gcctgcgaat gtgcctgtag gtgtggcgat
     2461 ggcggtattg atgcctatca gttcgccttg caggtttacg agggcaccgc cgctgttacc
     2521 cggatttacg gcagcgtctg tctgaataaa cgattcaacc ggagtgctgg cattttcacc
     2581 caggatatcg attttacgcc ccttggcgct cacgatacct gcggttacgg tgctggcaag
     2641 gtcgaagggg ttacccactg ccattaccca atcgcccacg cgcactgagt cggagtcgcc
     2701 gaacctgatg gcaggcaatc catccagatc gaccttcagg agggcgatat ccgtgcttgg
     2761 atcggttgct atcagctcag ccttcgccga gtgcttgtcg aagaacacca cttcgatatc
     2821 gtcggcattc ttgacaacgt ggtaattcgt cacgatataa ccatcttccg acatgatcac
     2881 gccggatcct gaggcttggg cacttggttg ctcccagttg aaatcatcgc caaagaactg
     2941 acgtatgatg tcgttggagc caccgtaatt tgcttgctgc ttgaaggtgg ttttcacgtg
     3001 cacaacagtt ggagttgtga gcgctgccga atacgtaaaa tcaacaggtg ccattcccga
     3061 cggcgctaca gggaatgaag ccttggtaaa cgcatatggc gaattactgc cattttcatt
     3121 gtggccgtag tcggcaataa accgatcgta caccaccacg gtagccaatg aacttatcac
     3181 cgccaccatc attgtcatcc cgagaatttt agctctcata atgcacttta ttttttttct
     3241 gattgttctc catcaaattt catgctctaa acgacgaatg atattaagtt gtattttcgc
     3301 ttaacaaatt ttaacctcat tatcgtttat taacgcaatt tatgcccatt cagttttaca
     3361 aatatcaggg aacaggcaat gattttgtac tatttgataa tcgcagtgga cagatagaca
     3421 ggacacgtta cgattggttt aattttattt gcgacaggcg atttggcatt ggtgcggatg
     3481 gggtgatgtt gctacaggag cgcgagggtt atgatttcga gatgttgtat ttcaatgccg
     3541 acgggcttcc gggctctatg tgcggaaatg ggggacgatg tatggtacag tttgcaaaac
     3601 gcctcggtat cgacaaagca caatatcatt ttcttgcttc tgacggcgat catgatgcca
     3661 tcattacggg cgacgaagtg tcgcttcata tgcgtgacgt gttgtcagtt gaactgcata
     3721 aaggacagta tattttaaac acaggatctc cgcatgtggt gttggctgtt gatgatgtaa
     3781 tggctgttga tgttaaccgc gacggagcac aattgcgata cgacccttta tttgcaccgg
     3841 gaggcacgaa tgtaaattat atacatattc agggtgaaac aatgcatatt cgcacctatg
     3901 aacgcggtgt ggagcgggag accttgagtt gcggaacagg tgtaacagca tctgcattgg
     3961 tttctgcttt gatgcaaaat cttcctttcg gcacccatga ggttcctatc cgcacaatgg
     4021 gaggagcgct gcgtgtgcaa ttccattatc atgccgacgg cagcttcacc gatatctacc
     4081 tgatcggacc cggaactttt gtttttagtg gggagataaa tgcttaaagc aaagaagttc
     4141 attcttcact gttgacccgt tccggtgcta atcgggttga gatatcaacc ggcatgaaac
     4201 cgggtgattt gcttaaattt aatcgagttc gaatgctaga cattaggatt gaattgccta
     4261 actttattga cgtagccgta aggatcggat ttatatgcga aaacacacca tcatatgtca
     4321 ataataaaga ctcctgatca gcgcgtcagg gtatttataa gttcaaccat caacgagtta
     4381 gcagaggaac gcatagctgc caggagtgca attgaaagct tgaaacttat tcctgttttc
     4441 ttcgaagccg gtgcgcgtcc gcatcctccg agggatttgt attcagccta tttaagccag
     4501 agtcatatat ttctgggaat ttattggaat agttatggtt ggatagctcc cggagctgag
     4561 atttcgggat tggaggatga atatcgactt tgcgggaaaa cgaagcctaa actgatatat
     4621 gttaagaatt caacagagcg tcaggaaaag ctagaccttt tattgcaaga tatttcgaat
     4681 agtgactcgg cctgttatca gaaatttaat actgcagatg agctcaaaca actgatagaa
     4741 aatgatctat cggtactgat gtctgagata tttgaatcat cactcatttc ggggcaggga
     4801 agtattgatt catcaacagg tgctatagaa aataagcatg atcatccgat aattgatatc
     4861 ccgcttatta aatcggaaat gatagggcgt gaacgagatt tgcaattagt ttctgaattg
     4921 ttgctaagag aggacacgag tcttattaca attctgggtg caggtggaac gggtaaaaca
     4981 acacttgcaa cgtttattgg tcagtcaaac aaggataaat ttaaagatgg ggttttattc
     5041 atacaattgg cagccatcac aaattctaaa ttggttgtaa acaccatagg cgaaaaatta
     5101 ggtatacagg ataatggcaa acatgattta atttcattga tcattgactt actcaccggc
     5161 aagcaatttt tgcttattct tgataatttc gagcaggtgg tagacgcttc ggtggttgtg
     5221 agccagctca tcaatcaatg taaggatttg aaggtattgg taactagccg cagtatttta
     5281 catatcagaa atgaaagaat ttaccacctg gcaacattaa atttccctgt tgaagagtct
     5341 gccatagcat ttgcggattt gcaaaattat gctgcaacga aattgttttt agatagggca
     5401 caggaagtaa atcaaaatat agaaaactca aaagaaaatg cggaagcaat acttgaaatt
     5461 tgtcagcgaa tggatggatt gccacttgca attgaactag ctgctgcaag aacaagattt
     5521 tttcaaccag ctgcattgct tggaagaatt acaaaaacac tagaccttgt aaataaagga
     5581 cataaggatt taccggagcg tcagcaaact ttaagggggg caattgaatg gagttataat
     5641 ttattgtcgc cggataccca aagtattttc agacaaatgg ctgtatttaa gcgcagttgg
     5701 accatggagg ctgctgatgc tatacttgta ataaatgaaa gtgcggttga tgccgaagaa
     5761 tatactgagc gacttttaga tgtaagtttg ataaaaacgg acatcttgaa taaagagttg
     5821 gaaccgagat tcaacatgtt acagacggtt catgaatatg ccggggaaat gctggcacaa
     5881 gatcccgatt gcgaaaaggt taaattgcag tatgccaagt atttcctcca attatgtgaa
     5941 gatgctgagc cctatttatg gaatcgaaac ggtgcaattt ggctcgaccg aatagaaagt
     6001 gaatatcaga atattcgcgc ttcattttat atttttatgg aattgggctt gcctgaaatg
     6061 gcttggcggt ttttcccttg tttagcgcca tactggacta ttcgcggggg attcacggaa
     6121 accgtgatgc tgataaaaga tgccggtttg tatgaagaat caagttggac tatcgggaca
     6181 attgatcctg tagtccatgg tcgcgcatta acctggagtg ggtattgtct gttatttgta
     6241 tttgaccttg aaagagggtt tgccatgttg caagctgcac aaaatatttt aaccgctacg
     6301 aatgatattt ataatttaac ctacgcatat tgttttgatg gatgttacgg cgcgtatatg
     6361 caaaaggaag acgccgaaga gaaaatatta aaggggtttg aattgttgga aaggcaaaat
     6421 aatcctttaa ttaactgtat gttttattgt tggtctgatg aatatttccg tcaaaaagga
     6481 caacctgaaa tcagagataa aaacttaaat ctcgcggctt cgattgctaa agagcatggt
     6541 atcacctata ttcaaggtgc cgtgatgttg atcagagcta caattgccac atctgaaccg
     6601 gaaatggatt cggataaatt aatacaaata ggacaggaga tgtttatgat ttttcctgaa
     6661 aaaggatacc agggatttaa ggctgcctca aagcaatttc aagcgatagg gtatttaaat
     6721 aagggagata ttattgctgc gcgaaatatg atcaaggagt ctgccgaatt tgttaaatta
     6781 tcaggtgaat tggaaagcga attttattta tgtatgctgc ttgtgcatat attggcctta
     6841 gggggcgacc gtaaacatgc ctttagtata tttggagcac tgcatacctt tatcgaacaa
     6901 tctcaatatc ctgttattgg tggggctgca gttcagtatc aaacaatatc ccaactgttg
     6961 cagccatatg aggcccagcc tgagtgtcaa tcctggtttg aatcgggaaa atcaatgcca
     7021 cttacctccg ctgtcaatat gggcttggct caaatttgat gtcggtattt tgtccttctg
     7081 attttaatag cactaccggc tattaccttc cctgtgcacc tcagcattaa gctatctcga
     7141 ctattcagtt atactccggc atagaggatt ttttaggtca gaaatgccga ttttgttaaa
     7201 gaaaaaccat atctttgcac acttaaaatt cagtatacgt tccagatgga aggagatgat
     7261 caaatactac aagaaaattg acaaccagat cgtagagatt gacagacctg aaaaagactg
     7321 ctggataaac gtataccccc ccttcgacca taaacgcctc gccaccctca gcgacgccat
     7381 cgacatcccg atagattatt tactcgactc catcgatatc aacgaacgct cgcgatttga
     7441 gcaggatgat aacgtgaagc tcatcgtgat caatacgcct atcgagaatg atatggagaa
     7501 ctccatcgac aacgatgcgt tttatatcac cattccaatc ggcatcatcc tgacgcagga
     7561 ttataatatc atcatcagct cggcacagaa taaagtaatc gactggtttt tcagtattgc
     7621 catcaaacat ttatcgcctc atgataaaga tttggtggta ttgaaaatat ttgaaaaaaa
     7681 tgtgttctat tttattcagt atctgaatga gatgaacaaa cgccgctacc tcatcgaaaa
     7741 agaattgatg cattcgagca gaaataccga gttggcgaaa ttgctcaata tccagaaatc
     7801 acttgtttac ttcgtaactg accttcgcgc caatgaatta ttgatgatga agattcagcg
     7861 tacgaatttc ctcggcatca aaaatgatga ggagaaaaat gattacctca ccgatatagt
     7921 gatcgacagc agtcaggccc tcgagatggc gacggtatac accaacatcc tcaatggtac
     7981 catggatgcc ttcgggtcta tcatctcgaa taatctgaac agtgtgatga agcggcttac
     8041 ttccatcacc atcatcctta tggtgccaac attgatagcg agtttctatg gcatgaactt
     8101 cgcgaatctt ccactggaga attccaatag cggattctac gtgatcatgc tgttctccat
     8161 tctgatcagc tttgccttgt attgggtatt taggagaatt cgctggttct gaattccttt
     8221 tctttaaatt tttattgctt aggaacgata tccatgcaga aatgtagcga tatccatatt
     8281 gcgtttgcct tcgggttgta ttgctaatac ataaacccat ccatcggcac ttgcaatctt
     8341 taaataggtc tttgcatctg ttgcaatagt gccgggagat tgttgatgtg gctctatttc
     8401 aaatttgctt ttgaggattt tccattgcgg acccttgatg tcggtaaatg caccgggata
     8461 aggggataat cctctgatta cattatgaat tcgttctgcc ggttggttcc aatcgatgcg
     8521 acaattttcg cggaatattt ttggggcgat tttattactt tctccggatt gcggtttcgt
     8581 ggaataatta ttttcctcga tggcaatcac tgtttttaca ataaggtcgg caccgagcat
     8641 catgagtgta tcgtggatgc tgccggcatt atcatcatca tgaatcggaa tgcgaatttg
     8701 atctatcaga tcgccggtat caatagcatg ttgaagaaaa aatgtggtag cacctgtttc
     8761 tttctcccca ttgatgatgg cgtgattgat gggagctgcg ccgcgataat caggtaataa
     8821 agatgcgtgt aggttgatgg tacctttagg cggcatgttc cataccactt cgggtaacat
     8881 tctgaatgcg acaaccactt gcagatcggc attgtaagtg cgcaatgtgg agataaattc
     8941 agggtctttc aatttcacgg gttgcaacac cggaatgccc tgcgaatcgg catacacttt
     9001 aacgggcgta ggtttaacct gcaatccgcg accacttggt ttatcgggag cagttacaac
     9061 ggcaacgaca ttaaatcctg cttccaccag tttttgtaat cccggcacgg caaaatcagg
     9121 cgttcccatg tatatgatgc gcaagtgatg agccattatt cgaaatcggg atatagtaaa
     9181 tattttttgc gcacctgttt aaaagcactg atatcatcct gccagcttaa gcgaatttct
     9241 tcttcactca atcctgcttt tatttgttgc atcaactctg cattgcccgc cagtttattg
     9301 aagaaattat tctccagaaa aaatggcgtt gatgatgcat aattatgata tacctccaga
     9361 aaaaatgcaa gatttatttt tccgctgcgc acactgtcga caggccattt agacagatca
     9421 tatccattgc aagtttgtcc catgaacgga ggtgttttcg cacccgatcc tgatttcggc
     9481 acaaaagaaa aaccggtagg gggtaaatat ggtgaaccga tatattcgaa aggtttgtcc
     9541 gttccgcggc caacactcac gttcgttcct tcaaacaagc ataaggttgg atataaatat
     9601 atcgcctgca tggtgcgcat gttcggactg gtaggaatat ctaagttgta aatatcggtg
     9661 tgatcgtaat tagcacatgg aataacttgt aattcgcatt gttcgccgtt tgtaagccag
     9721 tgttcaccat tcaccatgcg tgcatattca cctattgtca tgccgtgcac gatgggaatt
     9781 tgatgcatgc cgacaaacga agtatacgct ttttccaata ccggaccgtc aacataataa
     9841 ccattcggat ttggacgatc caggatgagg agtttctttt tattttctgc acatgcttcc
     9901 attacataat gcagcgttga aatgtaggta tagaatctgg cgccgatatc ctgaatatca
     9961 aatatgatga tatcagcgtc ggcaaggtct tgcggcagag gtttcttttt attgccgtag
    10021 agcgacaata ccggaatgcc tgttgcggta tccttggcat tcagaatggt agcacctgca
    10081 tcctccgtgc cgcgaaagcc atgttcggga gcaaaaatgg tactgatatg cacgccggcg
    10141 gcaatgagtg tatctacaag atgtttttta ccgattacag aagtttgatt taccaccagt
    10201 gcaacctctt ttcctttgag cattggcatc cacagttcag tttgttctgc accggtgagt
    10261 atggcatcat cattgtgcgt atcggtatgc acagtgtcga tggcggaaac cgtcacggaa
    10321 tcactatttt tacaggcagc ggtgtttagc agccctgcaa gcatcaaatt gaacaaaagc
    10381 atacgcatgt atagcaaatc aaaggccaaa ggtaaacgat aatcgggatg tggtttgaat
    10441 attttgtcag caatcgcatc atgcataata aggagcgtag cttttcgcga ctcatcatgg
    10501 gtatcgcgcg cgtggccatt gcattgagtt tatcggttat gctgatttct acatcgctcg
    10561 taaatggatt tcgtgcaacc atcagcgaaa aagtttatgg cttctgggga catatcaata
    10621 tcgcgaagca gagtttgcgc aattcatttg acgatattcc catt
//
LOCUS       DAOQOU010000112        10606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3263_length_10606_cov_5.510473, whole genome shotgun sequence.
ACCESSION   DAOQOU010000112 DAOQOU010000000
VERSION     DAOQOU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_10606_cov_5.510473"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..4585)
                     /locus_tag="PLM90_10035"
     CDS             complement(260..4585)
                     /locus_tag="PLM90_10035"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89757.1"
                     /translation="MKLRNLLFAVALLMTCISPVWADTEPDNDLAAGADAMTVGDPAT
                     GTINMSGDLIDFYAFTTSDDGTISLDLTSDVTYLTIRLYDADGVTVLQTASNYSNVSL
                     SRTGLAAGNYYAAVVAYAGYSGTYDITATVTAAPYMNDTESNNTYLLATDLTENGSTE
                     GHIGYRYNGGTYDTDDWYKVVTTNDGAITATFANTFGAYNTIYIYDNDGTTVLGSSSN
                     YGSSSITIPGKAAGTYYIRLYYYSTSHFNGYTLSNTVTPTAYDNDAEPNDLFSEATVT
                     IPENDSVSGHIGHRYNGGTYDTDDWYLLELTQDGQINLNLNYGFGQYHGIYLYDSDGT
                     TVLANNFNYNAATVTRNNLSAGTYYAKVDNYSSSYHSGYILEFEAIPAAYANDTEPND
                     VYTQANTDIPEGGTVYGHIGFYENGGTYDTDDWFTFTTTQDGIVSLTLNADPGQYHGI
                     YIYDNNGTTLLSNDFNYTSVTTTVNNLAAGDYYAKVDNYSASYHSSYALSLSVTPPAY
                     ANDPEPNGSLAEAVPMLTNSTVEGHIGYRNNGTIYDQNDYYVFTLSEPGDVTLSINKL
                     PGQYNTLYLLNSVGATLESQSNYGAFSITENDLPAGVYYARLHYYSTSHFGGYALTNS
                     YCPDVITIEATGETTFCDGESVVLSTPDHHLSYLWNDGSVTETNTVVISGDNFLTIDN
                     GGGCVRVSNIITTESTPLPVAIIETDGPAEFCDGGDVTLSVPALADTYLWSNGATTPT
                     ITVNETGDYSVILTKNGCTAISDPIHITVNALPVASVSADGMLTFCDGESVTLTANEA
                     AAYLWSNGETTASILVTGSGDYSVEITDDNGCMDMSEVTTVTVNANPIAAINADGPTT
                     FCSGESVTLTASGGDTYLWNNGETTSSILVTTSGLYSATVYTAAGCSDITSTIEVVVE
                     ACGSVTIVAEGPTEFCDGNTVTLTSSEITGNVWNTGETTQSIVVDASGDYYCTNGANT
                     SNTITVTVFANPIATITPDGATAFCAGGSVMLNASAAESYLWNTGATTASILADASGD
                     YSVTVTDINGCFATSMVTTVIEYTEPVATISAGGPTTFCEGGNVELTASEAESYLWNT
                     GATTESLTASSTGAYFVTITDINGCTATSGTITVTVNPNPTPVISADGPVLICGTDVT
                     LSADAVYDTYLWSNGETTPEITTGTSGVYSLTVTSGGCSGVSNTIEVVADVAPTVTIE
                     SESTLICFEASTMLTATTDGANIQWQRNGVDIPGATDAVYYASENGKYRAVVTTGACE
                     STSSEIKLKYAERLTISPAGVTDLCGGPVTMNIPAMPGLTYQWYRNNTLIAGATGNAY
                     TTTIPGKFYCLANADGCTRASKLLVVNDDCRNGNIVALEMSLWPNPASDYFTVSLQGM
                     SAGDAMLRVSDIAGQVIFTESVYINQIDQTFTIPSTDLPSGMYCVTFQDAPGNLTTQR
                     VIIQR"
     gene            complement(4693..5331)
                     /locus_tag="PLM90_10040"
     CDS             complement(4693..5331)
                     /locus_tag="PLM90_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ89758.1"
                     /translation="MDIRVVIFDDNRTRRDGVSLLLNTSDGYACVGAFEDFAKGLEQI
                     EQLMPDVIIMDIDMPGINGIEAVTRIKSRFPEQKVMMQTVFDTDDKVFESILAGASGY
                     LLKKASPVKLLESIREIHEGGSPMTPEIATKVLQFFRTRERQRGAGQQLLTAKEQEVL
                     SDLVNGLSYKMIADKQSSSYHTVNFHIRNIYKKLHVHSIGEAVAKAIRDRLV"
     gene            complement(5396..8473)
                     /locus_tag="PLM90_10045"
     CDS             complement(5396..8473)
                     /locus_tag="PLM90_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ89759.1"
                     /translation="MLTALATPAKAQKFPDLHFENIYTTDGLSDNSVTCLMEDSQGFL
                     WIGTSNGLNRYDGNTFDVFFHEEGNTNSLSGNIISGIIEDNTGVIWIGTRDGGITRYD
                     PQAKLSQRFTRFTHVPDDPKSIYSNRIITLAELNDTYIIFSTEGISSGFIDRKTYQIT
                     YHNISDTLSAITDPRTALPTPDGNSWVQRFQRVGSQIYLSRLIGGYAEVYDIEHPKRR
                     KLKSSGSAGSVQCFAIDDDRIWVGGWVPGLYVHPHPSSVPDGSILPVSKIVDVPSEVM
                     SIISWDAQYMLAASKGSGLYIVDKSTHAAKAFTYDRADPYSIAGNKIYCLLKDSRGIL
                     WVGTSNGLSKYHAGHWQFNPQLISTDFGQEITHFSMYEFHADKFGINTDQGMYVYTPS
                     TASIALHTFSFQGHSLNPTSVFRVDADKTYLTTESNIYHLDTSDLSIDILTPGMFCNP
                     AKDTCWIEKVHRLESYQFYKVLLDTLEDHPLHIFCSIGSGVGIYDDAEHVYYDIIQYT
                     NVPGTLSNNFVRDIYRDRSGNIWVATSEGLNKWNKSMPVKNTFTIFKYHAQDPHSISH
                     NNVSAIWQAPDGMLWLTTSNGLNAFDGKRFTRYYGNSNQFRQMYGIYPDKKGNIWIPT
                     RDGFEVFDLSKKTFRHVSMINPAWALKSPTRMLQTSSGTWMYGVGNYLISFTPDTYVF
                     DTTFPKLYIKELSVDDKHLFEELNMDALALRHNEDVISIVFSCLQLTHPGTAKYMYKL
                     VGQADSWIELGNNNAINFLDLSPGTYTLYIKVTNPQGDWSGPVKMLQFTVLPAFWQTW
                     WFYLLCTLSILGIGWAVVRYRELQLKRVLLMRNKIANDLHDDVGSALSTINIFGEVAA
                     KKSADNPELRSIIDKITDISTEMQENMTNIVWSLQPRNDNFDQMMLRIKSYALEHLAI
                     QQIDVHFSIDERLSGLKIAANKRKEMFLIYKEALNNILKYASATTVHISFKRNGADVV
                     MEITDNGKGFDPEELHAGNGMFTMRERAALIDGQLHIRSASGKGTSVILQCKC"
     gene            8652..9809
                     /locus_tag="PLM90_10050"
     CDS             8652..9809
                     /locus_tag="PLM90_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89760.1"
                     /translation="MKTLCLGIACLFCLHLNAQTIYAVDQSDGFKFGIIHPEDGSFTT
                     LHAFTENIYRINSGSASDPNAGYYFVEVKDENEDHFLYTIDITTGDVLYDPALSEPLF
                     ELNYSCHFDLLFGFEYTDTATNMVSINHMTGETTLLASYPEIDFLYMMTGSYNEKYSE
                     FLCWVKLVDDDNYYKTMFDIPTGEILLQVEESLQFENLYNCNDGLYYSFDWVDGIPWL
                     TTLDPYSGISTPIIEGAETNGILLSSTVLLPDENKFVAISDNLDYNYEYWVFDITGGT
                     FEVTEPVSGFMWPQEFLVSYCCAYLDIQTQTTTSPLTVYPNPANSYITITPQLLPNIE
                     RYTIISMNGSIVLEGDARMESVIDVSGLANGMYQLKVEMGDGVVSGMFVKE"
     gene            10141..>10606
                     /locus_tag="PLM90_10055"
     CDS             10141..>10606
                     /locus_tag="PLM90_10055"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HMZ89761.1"
                     /translation="MLRLFSPETLNLGGQFCKMPTFKLYIEKRKANKLGEAPIYLRII
                     KDRQKSLIAMGYKVKPEDWDEVKQRVRKSHPNSVRMNNLLAQKMAEVQGEAVKMEAED
                     KTANTKSLKRAARGTKGHSFLKYADRYLVELEHKAKTGTLDKAKAVIAKVKAY"
BASE COUNT         2933 a         2380 c         2398 g         2895 t
ORIGIN      
        1 tggatgacgg aggttatgca ggcgtgagct ttgggtttaa tgtggtgaag aaataaaagt
       61 gaaagccccc atctacaaag gactgtttgt tgatcaaaca gtcctttttt ttacaccgcc
      121 acccgtgtaa ccaccacatc agaaacatca tacatgttca ctatgcaaca tctgctatct
      181 gcataaggca tcaccatacc gaaaaaaggg ctgccttgat atacaaagca gcccattttt
      241 tacatctttg aaattaaaat caacgttgga tgatcactcg ctgagtggtc agattacccg
      301 gtgcgtcctg gaatgttacg cagtacattc cggaaggcag atcggttgat ggtatggtga
      361 atgtttgatc tatctggtta atatataccg actcagtgaa aatcacctgt ccggcaatat
      421 cactcacacg caacatggca tcaccggcac tcataccttg caagcttacg gtaaagtagt
      481 cgcttgccgg attcggccaa agggacattt ccaatgctac aatatttcca ttgcggcaat
      541 cgtcgttcac caccaataat tttgaggccc tggtgcaacc gtcagcattt gccagacaat
      601 agaatttacc cggaatggta gtggtatatg cattgccggt agcccccgca atcagggtat
      661 tgtttctgta ccattgatag gtaagtccgg gcatggccgg aatgttcatg gttaccggtc
      721 caccacagag atcagttaca cccgcaggtg aaatggtcag tcgttctgca tattttaatt
      781 tgatctcaga gctggtactt tcacatgcac cggtagttac cactgcgcga tattttccat
      841 tttcggatgc ataatacacc gcatccgtag caccaggaat atcaacgcca tttcgttgcc
      901 attggatatt tgccccatca gtggtagctg taagcatagt gcttgcttcg aaacaaatta
      961 aagtgctttc cgactcaatg gttacggttg gtgcaacatc cgcaaccact tctatagtat
     1021 tggaaacacc ggaacaacca ccagaggtaa ctgtcaaact ataaacaccg ctggtaccgg
     1081 ttgtaatttc tggtgtagtt tcgccattgc tccacagata agtatcgtag actgcatctg
     1141 cgctcagtgt aacatctgtt ccgcaaatga gcactggtcc gtcggcactt atcaccggtg
     1201 ttggatttgg gttaaccgtt actgtgatcg taccggaagt tgccgtgcag ccattgatat
     1261 cggtgatggt tacaaaatac gctcctgttg aagatgccgt gagcgactca gtagtagctc
     1321 cggtattcca gagatatgac tcagcttccg atgctgtcaa ttcaacatta cccccttcac
     1381 agaaagtagt tggaccaccg gcgctgatgg tggcaaccgg ttcagtatat tcaatcaccg
     1441 tagttaccat ggaagtagca aaacatccgt tgatatcggt aacagttacg gaataatcac
     1501 cactggcatc tgccaaaatc gatgcagtag tagcgcctgt attccagaga tacgactcag
     1561 cagcagacgc attcagcatc acgcttccac ctgcacaaaa agcagttgct ccgtcgggtg
     1621 tgatcgttgc gatcggattc gcaaacacgg ttaccgtgat cgtattcgat gtatttgcgc
     1681 cattcgtgca ataataatct cctgaagcgt ccactacaat gctttgagtg gtttcaccgg
     1741 tattccaaac gttgccggta atttcggatg aagtaagggt aactgtattg ccatcgcaga
     1801 attctgttgg cccttcagca acgatggtaa cgctaccgca tgcttcaaca actacttcga
     1861 tagtggaggt aatatcagaa catccggctg cagtataaac agtcgcagaa tacaaacctg
     1921 aggtagttac aagaatagac gaagtggttt ctccgttgtt ccacaaatac gtatcaccac
     1981 cggatgcagt taaagtaacg gattcgccgc tgcagaaagt ggttggtcca tcagcgttga
     2041 tcgcagcaat tggatttgca ttgacagtta ctgttgtaac ttctgacata tccatacatc
     2101 cattatcgtc ggtgatctcc acggaataat cgcctgagcc ggtaaccaga atggaggcag
     2161 ttgtttcgcc attgctccat aaataagcag cagcttcgtt agcagtaagt gtaacgcttt
     2221 cgccatcaca gaaagtaagc atgccatcag cagaaacgct ggcaacagga agtgcattta
     2281 ctgtaatgtg tatcggatcg gagatagcag tgcaaccgtt tttggtgagt atgacactat
     2341 aatcaccggt ttcatttacg gttatggttg gtgttgttgc gccattgctc cagagataag
     2401 tatccgcaag tgccggaacg cttagggtaa catcgccacc atcacagaac tcagcaggtc
     2461 cgtctgtttc gatgatggcc accggaagcg gcgttgactc ggttgtaatg atatttgaaa
     2521 cgcgcacaca gccgccgcca ttgtcgatag tgaggaaatt atcgcctgag atcactacgg
     2581 tattggtttc ggtgacagag ccatcattcc acaaataaga caaatgatga tcaggtgtgc
     2641 tgagcactac cgattcgcca tcgcaaaatg tagtttctcc ggtagcttca atggtgatca
     2701 catcagggca ataactattg gtgagtgcgt atccaccgaa gtgactggtg gaataataat
     2761 gaagtcttgc ataatacacg cctgcaggta ggtcattctc cgtgatggaa aaagcgccgt
     2821 aatttgattg agattccaaa gttgcgccca cactatttaa taaatacaag gtattgtact
     2881 gtccggggag tttattgata gaaagcgtta catcgccggg ttcactcagt gtgaatacat
     2941 aataatcgtt ctgatcataa atagttccat tgtttctata tccgatatgg ccttcaaccg
     3001 ttgaattggt gagcataggc actgcttctg cgagtgatcc atttggttcg ggatcgttgg
     3061 cataggcagg tggtgttaca gacaatgaca aagcgtaaga agaatggtat gatgcactgt
     3121 aattatccac cttggcgtaa taatcacccg cagccagatt attaacagtc gtagtaacgg
     3181 aggtataatt aaaatcattg gacagcaagg tggtgccatt attatcatag atataaatac
     3241 catgatattg tccagggtct gcattcaagg tcaggcttac gataccatcc tgagtggtgg
     3301 tgaaggtgaa ccagtcatct gtatcgtatg tgcccccatt ttcataaaat ccgatatgac
     3361 cataaacggt gccgccttca ggaatgtcgg tatttgcctg ggtgtataca tcattcggtt
     3421 ccgtatcatt cgcatatgcg gcaggaatgg cttcaaactc aagaatgtaa ccggaatgat
     3481 agctggaaga ataattgtca acctttgcat aataagttcc ggcggataaa ttattccggg
     3541 taactgtggc cgcattataa ttaaagttgt ttgcaagcac ggttgtacca tcgctatcat
     3601 ataaataaat accatgatat tgtccgaaac cataattcag attcagattg atctgtccgt
     3661 cttgtgttaa ttcgagcaga taccaatcat cagtatcata ggtgccgcca ttgtatcgat
     3721 ggccgatatg gccactcaca ctgtcatttt caggaatggt gaccgtagct tcagaaaaaa
     3781 gatcattagg ctcagcatca ttgtcgtacg cggttggtgt aaccgtattg cttagggtat
     3841 agccattaaa atgtgacgtg gaataataat acagtcggat ataataagta ccggctgctt
     3901 ttccaggaat ggtaatgctg ctactgccat aattagagct gctgcccaat acggtagtac
     3961 catcattgtc gtaaatataa atggtgttat acgccccaaa ggtatttgcg aatgtggcgg
     4021 tgatggcacc gtcattggtg gttacgacct tgtaccaatc atccgtatca taagtgcccc
     4081 cattataacg atagccgata tgtccttcag tacttccatt ttctgtcagg tctgtagcga
     4141 gcagataagt gttattactt tctgtatcgt tcatataggg cgctgcagtt actgtagctg
     4201 taatgtcgta agtgccacta tatccggcat aggctacaac agcagcataa taattaccgg
     4261 cagcaagtcc ggttctggat aaggaaacgt tgctataatt agaagccgtt tgcaacaccg
     4321 ttacgccatc cgcatcatac aggcgaatgg tgaggtatgt tacatcgctg gtcagatcaa
     4381 gtgaaatggt tccatcatca cttgtagtga atgcatagaa gtcgatcagg tcgcctgaca
     4441 tgttgatggt tcccgtggcc gggtcgccaa ccgtcattgc gtctgcccct gcggcaagat
     4501 cgttatcagg ttccgtatct gcccataccg ggctgatgca ggtcatgagt aatgcaacag
     4561 caaaaagtag atttcgaagt ttcataagaa aagattaggg ttcaataatt ccggggtatt
     4621 caatcctcaa aattatggct aacctgtatc cggtgtaacc gcaaaatcta gcaggtaagc
     4681 gcagtggtct gattatacca agcggtctct gatcgctttg gctactgctt caccaatgct
     4741 gtgtacgtgg agctttttgt agatattccg aatatggaaa ttaacggtat gatagctgga
     4801 gctttgcttg tcggcgatca ttttataact cagtccgttc accagatcac tcagcacttc
     4861 ctgttctttt gcagtgagta attgttgtcc ggcgccgcgt tggcgttcgc gtgtcctgaa
     4921 aaattgtaac accttggtag cgatttccgg ggtcatgggt gaacctcctt cgtggatctc
     4981 gcggatagat tctaacagtt ttacaggtga agctttcttg aggagataac cggatgcgcc
     5041 ggccaggatg ctttcaaata ctttgtcgtc ggtatcaaaa actgtttgca tcatcacttt
     5101 ctgttcaggg aatcgcgact tgatgcgggt aacggcctct atgccgttga taccgggcat
     5161 gtctatgtcc ataatgatca cgtcaggcat cagttgttcg atctgttcga gccctttggc
     5221 aaaatcctca aacgccccta cacaggcata gccatcggag gtattgagca aaagcgacac
     5281 gccatctctt ctggtgcgat tgtcatcaaa aatgaccact cggatatcca tagaaattca
     5341 ttacaccaaa aataaagcaa aacagtccaa tcctacccgc aaaatttagt aggtatcagc
     5401 atttacattg caatatcaca ctggtgccct tacccgatgc tgagcgtatg tggagttgcc
     5461 catcgatcaa tgctgcacgc tcgcgcatgg tgaacatgcc atttcctgca tgcagttctt
     5521 cgggatcaaa tcctttaccg ttgtcggtga tctccatgac tacgtcggca ccgttgcgtt
     5581 taaaggagat atgcacggtg gtagcagagg catatttgag gatattgttc agggcttcct
     5641 tgtagatcaa aaacatctcc ttccgtttat tggcagcgat ctttaaacca ctcagtcgct
     5701 catctatact gaaatgcaca tctatctgct gaatagctaa atgttcaagc gcatacgatt
     5761 tgatacgcag catcatctga tcaaaattat cattgcgtgg ttgcagactc cagactatgt
     5821 tggtcatgtt ctcctgcatc tctgtgctga tgtcggtgat tttgtcaatg atggatctta
     5881 actcaggatt atcggcagac ttctttgctg ctacttctcc aaatatgtta atggtgctga
     5941 gtgcgctgcc tacatcatcg tgcagatcat tggcgatctt gtttctcatc agcagcacac
     6001 gttttaattg caattcgcga tacctgacca ccgcccatcc tataccgagg atgctaagtg
     6061 tgcatagcag atagaaccac caggtttgcc aaaacgcagg caacacggta aactgcagca
     6121 tttttacagg accgctccaa tcgccttgcg ggttggtcac tttaatatat aaagtgtaag
     6181 tacccggcga caggtccagg aagttgatgg cgttgttatt tccaagctct atccagctat
     6241 ccgcctgccc aacaagcttg tacatatatt ttgccgtgcc gggatgcgtt aattgcaaac
     6301 aggaaaatac aatggaaatc acatcttcat tgtgacgcaa cgccaaagca tccatgttaa
     6361 gttcttcgaa caggtgttta tcatcaaccg ataactcttt aatatataat ttcggaaagg
     6421 tcgtatcgaa tacataagta tccggtgtaa aggagatgag gtaattgccc acgccgtaca
     6481 tccaggtgcc tgaggaagtt tgcagcatac gggtgggtga cttcaaagcc caggcaggat
     6541 tgatcataga cacatgcctg aaagtttttt tcgacaggtc gaatacttca aaaccatctc
     6601 tggtagggat ccagatattt ccctttttat ccggatatat cccatacatc tgcctgaact
     6661 gattggaatt tccgtaatag cgtgtgaatc gcttaccatc aaacgcattc aatccattgg
     6721 atgtggtcaa ccagagcatg ccatcaggtg cctgccagat agcagataca ttattgtgcg
     6781 atatggaatg cggatcctgc gcatgatact tgaatatggt aaatgtattc ttcaccggca
     6841 tggatttatt ccacttattc agtccctcag aagtagccac ccagatattt ccggaacgat
     6901 cacggtatat gtcgcgaaca aaattattgg aaagtgttcc cggcacgttg gtatattgaa
     6961 tgatgtcata atacacatgc tccgcatcgt cataaatacc tacaccggag ccgatagaac
     7021 aaaatatgtg taagggatgg tcttccagag tatcgagcag caccttgtag aactggtagc
     7081 tttccaaacg gtgcaccttt tcgatccagc aggtatcctt tgcagggttg caaaacatgc
     7141 ccggtgtaag gatgtctata cttagatctg aagtatctaa gtgataaata tttgattcag
     7201 tggtcaggta ggttttgtct gcatctacac gaaacacgga agtcgggttg agcgaatgcc
     7261 cttgaaaaga aaaggtatgc agtgctatcg aggctgttga tggcgtatac acatacatac
     7321 cctgatctgt attgatccca aatttgtcgg catgaaactc atacatggaa aaatgtgtga
     7381 tctcctgacc aaaatcagtg gaaatcagtt gcggattaaa ttgccaatgc cctgcatgat
     7441 atttgcttaa tccatttgat gtacctaccc aaagtatgcc gcgactatcc ttaagcaaac
     7501 aatagatctt atttccggca atgctatagg gatccgctct gtcgtaagtg aaagcttttg
     7561 cagcatgtgt agatttatct acgatataca atccactgcc tttggaagca gccagcatat
     7621 attgtgcatc ccaggaaatg atggacatca cttcagaggg cacatcaact attttgctta
     7681 cgggtaagat gcttccatcg ggtactgagg atggatgtgg atgtacatac aatcccggca
     7741 cccatccacc tacccagatg cggtcatcgt cgatggcgaa acactgcacg ctgcctgccg
     7801 atccgcttga ttttagtttt ctgcgtttcg gatgttcaat atcatacact tctgcatatc
     7861 caccgatcaa tcgggaaaga tagatctgcg aacctacgcg ctgaaacctt tgcacccagc
     7921 tgtttccatc cggtgtcggt aaggccgtgc gcgggtcagt gatcgcgctg agggtatccg
     7981 atatgttgtg ataggtgatc tgataggttt ttcggtcaat gaatcccgat gagatgcctt
     8041 cggtgctgaa aatgatatag gtatcattca gttcggccag ggtgatgatg cgattggaat
     8101 aaatactttt cgggtcatca ggaacatggg taaaccgggt aaatcgttgg gagagtttag
     8161 cctgcggatc atagcgggta ataccaccat cgcgggtgcc gatccagatc actccggtat
     8221 tatcttctat gataccggaa ataatattgc ccgagaggga attggtattt ccttcctcgt
     8281 gaaaaaatac atcaaaggta tttccatcat accgatttaa tccatttgag gtgccgatcc
     8341 ataaaaaacc ctgactatcc tccatcaggc aggtgacaga attgtcgctt aatccatctg
     8401 tagtgtagat gttttcaaaa tgcaggtcag gaaacttttg cgcctttgcc ggagtagcaa
     8461 gagcagttag caaaaggcat aataaacata tcctgtacat ggcgaaatac atgtcaggct
     8521 aagatacaaa ttaggcagaa aacccaattt cacgacaagg gatatacatg gtatatgcaa
     8581 tacacccaaa aaaacacgcc caataatgtg caacagttat gtatatttgt atagataaat
     8641 actgattacc tatgaaaaca ctctgcctgg gcattgcctg cctgttttgc ctgcacctga
     8701 atgcacaaac gatttatgct gttgaccaat ctgatgggtt caagtttggc atcatacacc
     8761 ctgaagatgg tagctttact accctccacg cttttacgga aaatatatat cggatcaact
     8821 caggaagtgc cagcgacccg aatgccggat attactttgt tgaggtgaag gacgaaaacg
     8881 aggaccactt tttgtatacg atagacatca caaccggcga tgtgttgtat gatcccgcat
     8941 tatccgagcc tttgtttgaa ctcaactaca gttgtcattt cgacctgctc tttggctttg
     9001 aatatactga caccgcaacg aatatggtca gcatcaatca catgaccggt gaaaccaccc
     9061 tgctcgcttc ctatccggag atcgatttcc tgtacatgat gaccggatcc tataatgaaa
     9121 agtatagcga atttttgtgc tgggtcaaat tggtggatga tgataattac tataaaacca
     9181 tgttcgacat accgaccggt gaaatattgc ttcaggtgga agaatccttg cagtttgaga
     9241 atttgtataa ttgcaatgat ggcctgtatt atagcttcga ctgggtggat ggtattccgt
     9301 ggttaacaac ccttgatcca tattccggca tcagcacgcc gatcatcgaa ggagcggaaa
     9361 ccaatggcat cctgctcagc tccactgtcc tgctcccgga tgaaaataag tttgtggcca
     9421 tttccgacaa tctcgactac aattatgaat actgggtatt tgatattacg ggaggcacct
     9481 ttgaagtaac agaaccggta tccggtttca tgtggccgca ggaattcctg gtgagctatt
     9541 gctgcgccta tctcgacatc caaacccaga ccaccacatc accattaacc gtatacccca
     9601 acccggccaa ctcctatatc accatcactc cccaactcct gccgaacatc gaacgctaca
     9661 ccatcatctc catgaacggc tctatagtgc ttgaaggaga cgccagaatg gaaagtgtga
     9721 ttgatgtttc aggtttagca aatgggatgt atcagttgaa ggtggagatg ggggatggtg
     9781 ttgtttctgg gatgtttgtg aaggaataaa cacatattac gatttaagga tatgacatca
     9841 atttagatac acctcatcgt cgagtataaa aaagagatca acttcagttg tgtaattaaa
     9901 atagtatgtg agatacgtac ttaattgcaa tagttaaata ataaccatca gaacgactta
     9961 aggaataaaa aaaacctccc gactgggagg tttacttttt tgcggagcgg acgggactcg
    10021 aaatattgca cccgtttaca atatatttca ccttattaat tgtttatcaa ttacttagat
    10081 aatttataaa aacacatata aagcattttg aagcaaggaa atgtgcgaaa tgttccgcct
    10141 atgttgcgcc tattttcgcc cgaaaccctt aatttaggcg gacaattttg caagatgccc
    10201 acatttaagc tctacatcga aaagcgcaag gccaataaac tcggagaagc ccctatttac
    10261 ctccgcatca tcaaagacag gcaaaaatcg ctcatagcca tgggctacaa ggttaaaccg
    10321 gaggattggg atgaagttaa gcaacgtgtc cgcaagtctc acccaaactc tgtccgcatg
    10381 aacaacctcc tggcgcagaa aatggcagag gtccagggcg aggctgtcaa aatggaggct
    10441 gaggacaaga cagccaacac caaaagcctt aaacgagccg ccaggggcac aaaagggcat
    10501 agtttcctga agtatgccga ccgctatctg gttgagctgg agcacaaagc caaaaccggc
    10561 accctggaca aggcaaaggc tgtcattgcc aaggtaaagg cctatc
//
LOCUS       DAOQOU010000113        10577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3273_length_10577_cov_6.379301, whole genome shotgun sequence.
ACCESSION   DAOQOU010000113 DAOQOU010000000
VERSION     DAOQOU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10577
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_10577_cov_6.379301"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2546
                     /locus_tag="PLM90_10060"
     CDS             <1..2546
                     /locus_tag="PLM90_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89762.1"
                     /translation="ISRLFPDGTPDPAFGTGGIAIQYVNGDGYGSPLMGSVEKVLIQP
                     DEKIVLVGQVVNTAVDEFNYDYFIMRLMPDGSRDATFGTDGFSMYGYFDEWGYFQRDY
                     ARSAALLPDGKIVIVGDTGPYGLSYASIMRILPNGLVDPTFGTDGSYFINVSDDSYGR
                     GIAVLGDGSMVMSGYFFDGQYVFFAKWNADGLMDETFGIAGIQIDSTIAGFNLKRMAI
                     TPDEKFILMGEKNSTVMINRYTSEGIADTTFAYFELPHSGTIYYAEDLFIDEENHTYF
                     CYHASNPKDYFLGKLTDVGGLDYSFNASGTAQFDLTTGISECQSVVMSGETLYASGYA
                     DFQNDYDIATIAVTTEGEIDNGFATDGVWRANVGRSADYGHAIALTPDNKILIAGNNG
                     FVLVARVNETGVLDSTFGNNGVASHALYYGISYPTVTSVLSLPNGRIVVLAYDDDLVV
                     SRLYPDGSLDPTFNGGYANLTVGDEELYYTYDLAYQEDEKVLFSGIYYGYGFAYEEAV
                     FLIGRLNEDGTFDSTFNGDGLNYDANFSESLNIARAIAVMPDDRIVAGGYYVNDNEKQ
                     YPRLVRYMPDGSRDNSFSGDGAFGATINGEEGRILDLKVDIENRIVGAGYVGAVENDT
                     VQIMVMRVTADGELDTTFADGGYFIYDTPLEASARSIAVDDSNRIVVSGYAYIEIGNA
                     AFFTLRLLSDGTPDPTFGINGMLFTDLTPEDDIAYSMQIQDDGKIILGGTSSENLNTN
                     FALVRLLPENGFTEPIDSDTTYGSFLVFPNPIHGAFVLQYQLLDAQQVSIGLFDAAGN
                     NVAQLMTNEARDAGDHQEALQLPETLARGYYFLRISCSDLVTMVQIVVM"
     gene            2620..3408
                     /locus_tag="PLM90_10065"
     CDS             2620..3408
                     /locus_tag="PLM90_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ89763.1"
                     /translation="MQLENLAIDIRENILYITINRPKNLNALNIKTIVELDEVFEEYM
                     YNDDVKGVIITGSGEKSFVAGADISEFADFTPEQGRTMSENGHDTFDKIEMYNKPVIA
                     AVNGYALGGGCELAMACHIRVASENAIFGQPEVNLGIIPGYGGTQRLVELIGKGKALE
                     LLMTGDSINAALAKELGLVNYVVSPEELMPKCEEIMKKIIGKGPVAVSKVVQCVNAHF
                     RGGGYVGDSYQTEITQFGQCMETEDFVEGTTAFLNKRKPEFKGK"
     gene            3554..4132
                     /locus_tag="PLM90_10070"
     CDS             3554..4132
                     /locus_tag="PLM90_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89764.1"
                     /translation="MRNYDWPQVYALVQAMTRNEKGYFRKLHEGFGGDSQRQYMQLYA
                     LLEKQKTLDLGAIKKAFAGQGINLTSTRNHLMQQLLRSLRNYSVGKSDLMQLRELLDY
                     AALLEKKGLHQACMELTKEGIDLAQKNNHPWYVILLLNIQRSIIDLMPENERDALAAA
                     IYQKTVEANKSLQTYRWDVGDKHRHKGKGNQN"
     gene            4236..4811
                     /locus_tag="PLM90_10075"
     CDS             4236..4811
                     /locus_tag="PLM90_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HMZ89765.1"
                     /translation="MKKRISLFLLTAILGFSHFSNAQVNAVKPFKTVIIGNQEWMSVN
                     WDYKTPKSFFYNNDSTIDSKYGRLYFYSNAVAAAPPGWHLPTLDEWWELMNYLGGINK
                     AAPLMMASDTIGLHLISGGYKSANISADNSDLFGFIDQYAFYWTATPDAEQTAYAIRI
                     TPSTGEIEVNSFRRANGFSVRYVKDKEVKED"
     gene            complement(4832..5467)
                     /locus_tag="PLM90_10080"
     CDS             complement(4832..5467)
                     /locus_tag="PLM90_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89766.1"
                     /translation="MKKILTVLFLFALSNYAFGQDQIDTYIKDAQTYIAAKDYKNAQL
                     SLQDAINELNNLIGQQIAESLPAEINGLKADGDATTNSAAMGMMGGGIMISKSYSNPT
                     NKNNDAEINIVANSPMIATMGMYMNNPAMLGEGYKSVKVGTQRAILKSEMEDRFVEDG
                     KPEIQIRSTEIQIPLGQTLITLQLSGFATEADELAFASKFDLAKLKTALGE"
     gene            complement(5570..7747)
                     /gene="recQ"
                     /locus_tag="PLM90_10085"
     CDS             complement(5570..7747)
                     /gene="recQ"
                     /locus_tag="PLM90_10085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HMZ89767.1"
                     /translation="MESMDAAKVLKRYFGYDAFRDQQEEVINHITSGHDTLVVMPTGG
                     GKSVCYQIPAMMLEGMAIVVSPLIALMKDQVDALVTNGINAVALNSSLTPDEQRIVTQ
                     KILRGEIKLLYIAPERLMGDEGRLIELLSTLKISLIAIDEAHCVSMWGHDFRPEYLKL
                     AKIREKLTRVPIIALTASADAQTRADILDKLNMPKAHISISSFNRANINYYIEQKRDA
                     YTAIINYIVQHQGQSGIIYTLSRANAEMLAERLTADGFEAAYYHAGMDAASRNTVQEA
                     FKKDKTNIIVATIAFGMGIDKPDVRYVIHYNLPKNIESYYQETGRAGRDGLPSDAILF
                     YSYGDLMQLRRFAEVEGNPERTRIMLDKLMRMNAFCVSNICRRTMLLDYFGEKYPESN
                     CGSCDVCLSRKTSFEATTIAQKALSAIVRTEERFGIHYLVDFLRGSRSEKIWKGHKQY
                     KTYGIGADISRDKWMQYFQELIDQQYVKRIAEGSYPRLLITEKGKRVLQGTEQVYFTL
                     SEERLMVENSNQIEYDSHLFDLLRSLRTRIAYRESLPPYMIMGDSTLQEMAAYLPVQI
                     EDLKDISGFGEVKIGKYGPQFIEVIRQHVKDHALMTKMSSKRKSKSPPKPRTKTRPGI
                     NTSEQETLEIFQTGKSIGEIAAIRELSPNTIEGHLTECLAEGLISVEKFISPDKMKRI
                     IQVRQENPDAKLTILKEALGDAYSYFEIRVTLTAMKRKEKESS"
     gene            7942..9105
                     /locus_tag="PLM90_10090"
     CDS             7942..9105
                     /locus_tag="PLM90_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ89768.1"
                     /translation="MKRILTVLIALLPLINFAQDVQFFQGTWEEVNAKAKAENKLIFV
                     DAYTYWCGPCKMMDETMFHNNKEVADYVNAHFIAYKVECEHDFGVEFARKYKVNVYPT
                     MLMFNSSGQLLEKHLGYNDVQSEFIETFRKVVNMDQTDVYAMDPAKIKMNWPEFYAKN
                     FKDANDPTWKRDKSVDPNIWLDQQTDLFNEVSWAVMYIFNTNEKYSKFFLDNYETYKA
                     KYKFEAQDEINNIIYGFMNTAVKENKPELMTSAENLMQKYFPEDTSGLFYLRLNYMMS
                     VGDNNGLANLMSNYIATNKEVYLGTVNAAAWNLYENCDDQKILQTAISWFQPYLPTIT
                     DYFAMDTYAALLYKTKQYASAEEWAIKAIALGKADGEDMAGTEELLEKIKKEK"
     gene            9186..9908
                     /locus_tag="PLM90_10095"
     CDS             9186..9908
                     /locus_tag="PLM90_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89769.1"
                     /translation="METGQRKFGKTLLPTKNYMQVALIEIMLIFSVLLMGDIAAALGS
                     SSTGSAVSMALLIPEAVYVYILFRLTTLLTDNRVMQRIVAVLLVAAAILSVVGLLPLM
                     SGDESKSQVLIAVHLILCFIECYLIALGLQDIYIDKLTLRERLWGSVAMYLLIAIGWA
                     SFYEIFLLTDHTLFGVELIPGYQTYSEALYYSLCSLSGTGSIYSNPNHLIRNLSLIES
                     VWGVLFLVMLIGRVFTVSDKEE"
     gene            9990..>10577
                     /locus_tag="PLM90_10100"
     CDS             9990..>10577
                     /locus_tag="PLM90_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89770.1"
                     /translation="MKKLLLLLLLGVYTGIFAQSGSMEFTSSGTFTVPDGVTTIFIEV
                     VGAGGSGGYNGTGGGGGGGYASGNYSVTPGQVIAIAVGSPGVSPMVGTTSVEGFLSAS
                     GGENGVSVPNPEIGGGGDGGIGAGGNIVNHTGGNGGGGYYTYFGGGGGGAAGAVGDGA
                     DGGNTIAWTGICLTPGGDGGISGGAPGGNGGKGAGF"
BASE COUNT         2926 a         2032 c         2386 g         3233 t
ORIGIN      
        1 ccatatcgag attatttccc gacggcaccc ctgaccctgc atttggtacc ggcggcattg
       61 caattcaata tgtaaacggc gatggttatg gctcaccgtt aatgggcagt gtagaaaaag
      121 tattgataca acctgatgag aaaattgtgt tggtggggca ggttgtgaat acggctgttg
      181 atgagtttaa ttatgactat ttcatcatgc gactaatgcc tgatggctca cgagatgcta
      241 cattcggcac agacggattt tctatgtatg gttattttga tgaatggggt tattttcaac
      301 gagattacgc acggtctgcc gctttgctgc ctgatggaaa aattgtgatt gttggtgata
      361 cgggaccata cgggctcagc tacgcatcta ttatgcggat acttccgaat ggactggttg
      421 atccaacttt tggcaccgat ggcagttatt ttattaatgt aagcgatgat tcatatggcc
      481 gcggtattgc tgtacttggt gatggcagta tggtcatgtc cggatatttt tttgacggtc
      541 aatatgtgtt cttcgcaaaa tggaatgctg atggcttgat ggatgaaact ttcggtatag
      601 ccggcatcca aatagattct accatagcgg gcttcaacct taaaagaatg gcaattactc
      661 ccgatgaaaa atttatttta atgggagaaa agaattccac agtaatgata aacagatata
      721 catcagaggg aattgcagat actactttcg cctattttga actgcctcat tcgggtacta
      781 tttattatgc cgaagatttg tttattgatg aagaaaacca cacatatttt tgttatcatg
      841 catcaaatcc aaaggattat tttcttggca agctgacaga tgtcggcggt ttggattatt
      901 ccttcaatgc ttcaggtact gcccaattcg atctcacaac cggtataagc gaatgtcaaa
      961 gcgtagtgat gtcgggtgaa actttgtatg cttcaggata tgcagatttt caaaatgatt
     1021 atgatattgc cacaattgct gtaactactg aaggtgaaat tgataatggc ttcgcaacag
     1081 atggtgtgtg gcgcgcaaat gtcgggcgtt ctgctgatta tggacacgcc attgcattga
     1141 caccggataa taaaatttta attgccggaa ataacgggtt tgtgcttgtg gcaagagtaa
     1201 atgaaacggg tgttttggat tctacctttg gaaataacgg tgttgcttca catgcattgt
     1261 attacggaat tagctatccc acagttacaa gtgtactgag cctgccaaat ggcagaatag
     1321 tcgttctggc ctatgacgat gatctggtag tttcaagatt atatcctgat ggtagtttag
     1381 atccgacttt caatggtggc tatgccaatt tgacggtagg ggatgaagaa ttatattaca
     1441 cttacgacct cgcatatcag gaagatgaaa aagtgctttt ttccggaatt tattatggtt
     1501 acggtttcgc ttatgaagaa gctgtttttt taattggaag attaaatgaa gacggcactt
     1561 tcgattcaac gtttaatgga gatggtttaa attatgatgc aaatttttct gagagtttaa
     1621 atattgcccg tgcaattgcc gtaatgccgg acgatagaat tgttgccgga ggatattatg
     1681 tcaacgacaa tgaaaagcaa tatcctcgct tggtgcgcta tatgcctgac ggatcgcggg
     1741 ataattcatt tagtggggat ggtgcatttg gtgctacaat caatggtgag gaagggagaa
     1801 ttttagactt gaaagttgat attgaaaatc gcatcgtcgg agccggttat gtaggagcag
     1861 ttgagaatga cactgtccaa atcatggtga tgcgtgtaac tgccgacggc gaattggata
     1921 ctacttttgc cgatggcggc tattttattt atgatacgcc actcgaagct tcggcgcgat
     1981 caatagcagt tgacgacagc aacagaatag ttgttagtgg atatgcttat atcgaaattg
     2041 gcaatgccgc atttttcacg cttcgattat taagtgacgg cacacctgat ccgacatttg
     2101 gcatcaatgg catgctgttt actgatttaa cacccgaaga cgacatcgcc tatagcatgc
     2161 aaatacaaga tgatggtaaa attattttgg gcggaacatc cagtgaaaat ctgaacacca
     2221 attttgcatt agtacgtcta ttgcctgaaa atggatttac tgaacccatt gatagcgata
     2281 ccacttatgg ttcattttta gtttttccaa atcctattca cggtgctttc gtattgcaat
     2341 atcaattgct tgatgcgcag caggtttcaa taggattatt tgatgccgcg ggaaataatg
     2401 ttgcacaact tatgaccaat gaagcacgtg atgccggcga ccatcaggaa gcattgcaat
     2461 tgcctgaaac actggcaaga ggatactatt ttcttcgcat ttcgtgcagc gaccttgtaa
     2521 caatggtgca aattgtggtg atgtagttaa cacccaatgt gcatcacgca ccatggaaag
     2581 tagaattgct tcttatcttt gccacaaaca ctgctgataa tgcaactcga aaatcttgcc
     2641 atcgacataa gggaaaacat tctttacatc actattaaca ggcccaagaa cctgaatgca
     2701 ctcaatatta aaactattgt tgagttagat gaggtattcg aggaatacat gtataacgac
     2761 gatgtaaaag gtgtgattat aacaggttcc ggcgagaagt catttgttgc aggtgccgat
     2821 atcagcgagt ttgccgattt caccccggag cagggaagaa ccatgagcga aaatggtcat
     2881 gatactttcg ataagatcga gatgtataac aaacctgtca ttgctgcagt gaatggttat
     2941 gccctcggcg gtggctgtga attggctatg gcatgtcata tccgagtggc ttctgaaaac
     3001 gcgatctttg gtcagccgga agttaacctg ggtatcattc cgggttatgg cggaactcag
     3061 cgtttggttg agcttatcgg aaaaggtaag gcactggaac tcctcatgac cggcgattct
     3121 attaacgctg ctttggcgaa agagctcgga ttggttaact acgtggtttc accggaagag
     3181 ttgatgccca aatgtgagga gatcatgaaa aagatcatag gcaaaggtcc ggttgcggtg
     3241 agcaaggttg ttcaatgcgt aaacgctcat ttccgcggcg gcggctatgt tggcgatagc
     3301 tatcaaacgg agatcacaca gtttggacaa tgcatggaaa cggaagattt tgttgaaggt
     3361 actaccgcct tcctcaataa gcgcaaaccc gagtttaagg gtaaataagc gttatcattt
     3421 tggctttaac aatgtctgtg acctaatcac gggcagataa ccatatatct taagtaatag
     3481 atttcgtatc ttggctattc aatttcatta gcttgtattt caacaagtta caattgaagt
     3541 tgttatattt cgaatgcgca attatgattg gccacaggta tatgcactcg ttcaggcgat
     3601 gacgcggaac gaaaagggat attttcgaaa attacatgag gggttcggag gcgacagtca
     3661 acgtcagtat atgcagttgt atgcattgct cgaaaaacaa aagactcttg atttgggtgc
     3721 aatcaaaaaa gcatttgcag gtcagggaat aaacctcacg tcgacacgca accacctcat
     3781 gcagcaattg ttgcgaagtt tgcgcaatta ttccgtgggg aaaagtgact tgatgcagct
     3841 tcgcgaatta ttagattatg cagcattgct ggaaaaaaag ggattgcatc aagcttgtat
     3901 ggaattgaca aaggagggta tcgacctagc ccaaaaaaat aaccatccat ggtacgtgat
     3961 tttattgctc aacatacagc gttcaataat agatttaatg cctgaaaatg aacgcgatgc
     4021 tctggcagct gccatttatc aaaaaacggt tgaagcaaat aagtcactac aaacctatcg
     4081 gtgggatgtg ggagataagc atcggcataa gggaaaagga aatcagaact aacaggttac
     4141 ggcaaggggc tactgtatgg tgcccaaccg gtttgccgat taacattgga tggagtaatc
     4201 agaaaaaagg cgcgatattt gtatgaatta atgctatgaa aaagcgaatc agtctgtttt
     4261 tattgaccgc aattcttgga ttttcccact tttcgaatgc ccaagtaaat gccgtaaagc
     4321 cttttaaaac cgtaataatc ggtaaccagg aatggatgtc ggtgaactgg gattacaaaa
     4381 cacctaaaag ctttttctat aataatgatt ctaccataga cagtaaatac ggccgactgt
     4441 atttttatag caatgcggta gctgctgcgc caccgggatg gcatttgcca actttggatg
     4501 aatggtggga attaatgaac tacctcggag gtatcaataa ggctgcaccg ctgatgatgg
     4561 cttctgatac cataggtctc catctgattt ccgggggtta caaaagcgct aatatatcgg
     4621 cagataatag cgatctcttt ggctttatcg accaatatgc tttttattgg accgcaacac
     4681 ctgatgctga acaaacagct tatgctatac gtattacgcc ttcaaccggc gaaattgagg
     4741 taaattcctt ccgtcgcgca aatggttttt ctgtgcggta tgtgaaagac aaagaggtga
     4801 aagaggattg atatcccaac gccttttatc cttattctcc caatgccgtt ttcagttttg
     4861 caagatcgaa ttttgatgca aatgctaact catctgcctc agtagcaaat cccgatagct
     4921 gcagtgtaat caatgtttgt cccaacggaa tttgaatttc ggtagaacga atctgtattt
     4981 caggtttgcc gtcttcaaca aagcgatctt ccatttctga ttttaaaatc gcgcgctgtg
     5041 tgccaacttt cacactttta tatccttcgc caagcatagc aggattgttc atgtacatgc
     5101 ccatggtggc tatcatcggt gagttagcca cgatattgat ttcggcgtca ttatttttat
     5161 tggtaggatt ggaatatgat ttactgatca taatgccacc acccatcata cccatggccg
     5221 cggagtttgt tgttgcatca ccatcagctt ttaatccatt gatctcggca ggtaatgatt
     5281 ctgcaatttg ttgtccgatc aaattattca actcattgat ggcatcctgc aaactgagct
     5341 gtgcattttt gtaatctttg gctgcaatat atgtttgtgc atctttgata taggtgtcta
     5401 tctggtcttg tccaaatgca tagttgctta atgcaaacag gaatagcacc gtcaatattt
     5461 ttttcatata cgtggatttt atgtttagta aaggtagggt acaatccaat tccctgagca
     5521 attaatatta tgatattaat cactctgcaa catgggcatt tgcttattat caggacgatt
     5581 ccttctcctt gcgcttcatt gctgtcaggg taacccgtat ctcaaaatag ctgtaggcat
     5641 cacccagcgc ttcttttaat atggtgagct tggcatcggg gttttcctgt cgcacttgga
     5701 tgattctttt cattttgtca ggactgatga acttttcaac tgatatcaaa ccctctgcta
     5761 agcactcggt aagatgtcct tctatggtat tgggcgataa ttcgcgaatg gccgcaattt
     5821 cgcctatgct ttttcccgtt tgaaagattt ctaaggtctc ctgttcagag gtgtttattc
     5881 ccggacgtgt ttttgtcctc ggtttcggcg gcgatttgct tttgcgcttg gaactcattt
     5941 ttgtcattaa agcatgatcc ttcacatgtt gcctgatcac ttcaataaac tgcggtccgt
     6001 atttgccgat tttcacttcc ccaaatccgc tgatatcttt taaatcttca atttgcacag
     6061 gtaagtaagc cgccatttcc tgcaaagtgc tatcgcccat gatcatatag ggcggtaaac
     6121 tttcgcgata ggcaatgcgt gttcgcagac tgcgcaggag gtcaaacaga tggctgtcat
     6181 attctatctg attgctgttt tctaccatca aacgttcttc cgacaaggtg aaatatacct
     6241 gctcggtgcc ttgtagaaca cgcttgcctt tttcagtgat gagcaatcgc gggtaactgc
     6301 cttctgcaat gcgttttaca tattgctgat cgattaactc ttgaaaatac tgcatccatt
     6361 tatcgcgtga aatatctgca ccaatgccat aggttttata ttgtttatga cctttccata
     6421 ttttttcact gcgacttcca cgcagaaagt ctacgagata atgtattcca aatcgctctt
     6481 cggtgcgcac aatggcactc aatgcttttt gtgcaatggt ggtagcttcg aaagatgttt
     6541 tacgacttaa gcatacatca catgatccgc aatttgattc gggatatttt tctccgaaat
     6601 aatcgagtag cattgtgcgt cgacaaatat tcgatacgca aaatgcattc atgcgcatta
     6661 atttatccag catgatgcgg gtgcgttcag gattgccttc tacttcggca aatcttcgca
     6721 attgcatcaa atcgccataa ctataaaata gaatggcatc actaggcaaa ccatctctgc
     6781 cggcgcgtcc ggtttcctga taatatgatt cgatattttt cgggaggttg taatgtatga
     6841 catagcgcac atccggttta tcaattccca taccgaatgc aatagtggca acgatgatat
     6901 ttgttttatc ctttttaaat gcctcctgaa cagtattgcg cgatgcggca tccattccgg
     6961 catggtaata tgctgcttca aatccatctg ccgtcaatcg ctcggcaagc atttcggcat
     7021 tggcgcgact taatgtataa attatgccgc tttgcccttg atgttgcacg atgtaattaa
     7081 tgatagcagt ataggcatcg cgtttttgtt caatgtaata attgatgttt gcgcgattaa
     7141 aactggatat tgaaatatgt gctttcggca tatttaattt atccagaata tccgccctcg
     7201 tctgggcatc ggcactggct gtaagagcga taattggcac ccgcgtaagc ttttctcgaa
     7261 ttttagcgag ttttaaatat tccggtctga agtcgtgtcc ccacatgctt acacagtgcg
     7321 cttcatcaat ggctatgagg ctgattttca gggtagataa gagctctatc aacctacctt
     7381 catcacccat taatcgctca ggtgctatgt ataataattt gatttcgcca cgcaatattt
     7441 tttgcgttac tatgcgctgt tcatcgggtg ttaaactact gtttaaggca accgcattaa
     7501 ttccgtttgt caccagtgca tccacctgat ctttcatgag tgcaatcagc ggggaaacca
     7561 ctattgccat gccttccagc atcatggccg gtatttgata acagaccgat tttcctccgc
     7621 cggtaggcat aacaaccagt gtgtcatgac cacttgtaat gtgattaatt acctcctcct
     7681 gctgatcgcg aaacgcatca tatccgaagt agcgtttcag cacttttgcc gcatccatcg
     7741 attccataac ttgcaaatgt aggacgattc atcatactag tccgcttata agcgtttata
     7801 tgcgaataaa agcggttatg ataggttaat gatcttgttt ttcgcggaaa ttaattgtca
     7861 tttgattgtc tgcccgcccg aaacgcttgc ttttgttaca taggtttgat ttactttgta
     7921 tcgttaaata tttattgaat tatgaaaaga atactgacag ttctaatcgc actcctcccg
     7981 ctgatcaatt ttgcgcagga cgtgcaattt ttccaaggca cctgggaaga agtaaatgcc
     8041 aaagcgaagg cagagaataa actcattttt gtagacgcct atacctattg gtgcggccct
     8101 tgtaaaatga tggatgaaac catgttccac aataataaag aagttgctga ttatgtcaat
     8161 gcacacttta ttgcatacaa ggtggaatgt gagcatgatt ttggcgttga atttgcgcgg
     8221 aaatataaag tcaatgtata cccaaccatg ttaatgttta acagctcagg tcaattactt
     8281 gaaaaacatt taggatataa tgatgttcaa tctgaattta tagagacctt caggaaagtg
     8341 gtgaatatgg atcaaacaga tgtttatgca atggatcctg caaaaataaa aatgaactgg
     8401 cccgaatttt atgccaagaa ttttaaagat gccaatgacc ctacctggaa acgcgataaa
     8461 tctgtagatc caaatatttg gctcgatcaa cagactgatt tgtttaatga agtgtcgtgg
     8521 gcagtgatgt atatttttaa tacgaatgag aaatactcaa aattcttcct cgataattat
     8581 gaaacctaca aggcaaaata taaattcgaa gcgcaggatg aaatcaataa tattatttat
     8641 gggtttatga atactgcagt taaagaaaat aagcctgaat taatgaccag tgctgaaaat
     8701 ttaatgcaaa aatattttcc tgaagacact tccggattat tttacttgcg attaaattac
     8761 atgatgtcag tcggcgataa taatggtttg gctaatctta tgtcgaatta cattgctacg
     8821 aataaagaag tatatttagg caccgtaaat gctgcggcat ggaatttata tgaaaactgc
     8881 gatgatcaaa aaattcttca aacagccatt tcatggtttc agccctatct gccaactatt
     8941 accgactatt ttgccatgga tacctatgca gccttgttgt ataaaacaaa gcaatatgct
     9001 tcggctgaag aatgggcgat aaaggctatt gccctaggta aagctgatgg tgaggatatg
     9061 gccggaacgg aagaactcct cgaaaaaata aaaaaggaaa aatagcgctg tccgaatttc
     9121 tttatataag agtcgatctt ttccgggtcg gctctttttg ttaatattta gtgtaatttt
     9181 atcttatgga aacagggcaa cgcaaattcg gaaagacttt attgccgaca aaaaactata
     9241 tgcaggtagc acttattgaa atcatgctga tattttcagt attattaatg ggtgatatcg
     9301 ctgcggcgct tgggagcagc tctaccggtt cggcggtttc tatggccttg ttaatcccgg
     9361 aggctgttta tgtatatata ctttttagat tgacaacctt attgaccgat aatcgtgtta
     9421 tgcagcgtat cgttgctgtg ttattagtgg cagcagcaat attgtcggtg gttggcttgc
     9481 ttcctctgat gtcgggagat gaaagtaaaa gccaggtgtt gattgctgta cacctgatat
     9541 tatgctttat tgaatgttat ctgatagcgc ttggattgca ggatatttat atagataaac
     9601 tcaccctgcg tgaacgttta tggggaagtg tggcgatgta tcttttgatt gcgatagggt
     9661 gggcgagttt ttatgaaata tttttgttga cagatcatac cttatttggt gttgaattaa
     9721 ttcccggtta tcaaacatat tctgaagcct tgtattacag cctctgcagt ttaagcggaa
     9781 cgggaagtat ctattcaaac cccaaccacc tcatacgcaa cctatcatta atagaatctg
     9841 tttggggtgt attatttctt gtaatgttga taggacgcgt atttacagtg tcggataaag
     9901 aggagtaatg tgttaaatgt tttccttgct ttcatccggc aatcatcaca tcatttgttt
     9961 gattatattt atctcctgat cttcaaacaa tgaaaaaatt actactcctc cttttgcttg
    10021 gtgtatacac cggcatattc gctcaatccg gcagcatgga atttacatct tccggaacat
    10081 ttacggttcc cgatggcgta acaacaatat ttattgaagt tgttggcgcg ggcggaagcg
    10141 gtggatacaa tggaaccggc ggcggtggcg gaggtggtta tgcaagcgga aattattccg
    10201 ttacacccgg acaagtaatt gcaatcgctg tcggttcacc cggtgtgagt ccgatggttg
    10261 gaactaccag tgttgaaggt tttctgagtg cttcaggcgg agaaaatggc gtatcggtac
    10321 ctaatcctga aattggtggt ggcggtgatg gtggcattgg tgcaggagga aatattgtca
    10381 atcataccgg cggaaacggg ggtggcggtt actatactta tttcggtggc ggaggaggtg
    10441 gagccgcagg tgctgttggc gatggcgctg atggcggtaa taccattgcg tggacaggta
    10501 tttgtctcac acccggtggt gatggcggaa tcagtggagg tgctcccgga ggaaacggag
    10561 gaaaaggcgc cggattt
//
LOCUS       DAOQOU010000114        10505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3305_length_10505_cov_6.017799, whole genome shotgun sequence.
ACCESSION   DAOQOU010000114 DAOQOU010000000
VERSION     DAOQOU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3305_length_10505_cov_6.017799"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLM90_10105"
     CDS             complement(<1..593)
                     /locus_tag="PLM90_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89771.1"
                     /translation="MMKRNAPGWILALVTAVIICAAWQKIIFSPNHYLLTTGGDAFKN
                     YYTPAWYVANDKGDRFTGMNYPYGEHVVFTDDQPMISWLINVIDDHVFPVHDNTVAIL
                     HLLMFISLWAGVLLIFHILRHYRMPAWYAAGMALLIGLMTPQLDRFWGHFALSYALFV
                     PLIWWLMIRLEKKNYTFRRAALLVAVVIFFGFIHMYYA"
     gene            complement(590..1315)
                     /locus_tag="PLM90_10110"
     CDS             complement(590..1315)
                     /locus_tag="PLM90_10110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ89772.1"
                     /translation="MSQPTLDIILPCYNPAPSWVDHIITGYGEIAAVIPHADIRVILI
                     NDGSSRSIGDEDLLQLKSAIAKFEFISYQKNRGKGYALRQGVAASNADITIYTDVDFP
                     YTTASFIQLYTVLANKEADVAAGVKDATYYAHVPAFRRFISKLLRACTTTVLRLKVSD
                     TQCGLKGFNDRGRNMFMRTSIDRYLFDLEFIFLASRNKSLKIKPVEIALRDNVQFSSM
                     RMSILLQESGNFLKILFRSIFTR"
     gene            complement(1442..2383)
                     /locus_tag="PLM90_10115"
     CDS             complement(1442..2383)
                     /locus_tag="PLM90_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ89773.1"
                     /translation="MKKVHYIILGAVIGVGLIVALAVKGNANGNGAHVESAMPTDTLR
                     SLADEVNTIRYFAAKLPKTLTFAGEEVPLDDPEVRERLDRELTVTGYWHSNTIQNLKL
                     ANRWFPIIESTLKKNGVPEDFKYLAMAESGLRNVISPSGAHGYWQFLENTGEHYGLTI
                     NGEVDERYDMEKSCEAASQYFKDAYAKFNNWTLAAASYNAGMGAIEGAVNYQDEKSYY
                     DLYFREETSRYVFRALAFKIIYEHTQDYGFFLTKEDLYDPLQYKEVTVTTSIPNLSEF
                     AKKNGTNYKMLKYYNPWLRSDMLTVKAGQQYRIKIPA"
     gene            complement(2380..2892)
                     /locus_tag="PLM90_10120"
     CDS             complement(2380..2892)
                     /locus_tag="PLM90_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HMZ89774.1"
                     /translation="MELVNKVAQSGLLTFDLEDLFPAEGSIVSFDLKDYLFRGLILRE
                     KDFREALAAIDWQQYAGKTVAVYCSADAIIPNWAWMLVAANVAGIAEMVVMGDPELAM
                     KRYYTQQLSQVDLEQYRDQRVVIKGCSDKPVPAEAYLEITALLRPVTKSIMYGEPCST
                     VPVFKKKEQA"
     gene            complement(3034..5217)
                     /locus_tag="PLM90_10125"
     CDS             complement(3034..5217)
                     /locus_tag="PLM90_10125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ89775.1"
                     /translation="MLISKIRQYSWLAVGLIALCLIGFLVQDATNSNTGMFRGNKAPD
                     YATMYGEEVNRDEVAERSGRTLLEYLTLQTGQVLQYENGSMQLDPQTKFGLNEQSWTE
                     YVNEKIIDKQLEELGLGVTEEELTNLIYGPDPHPYIKNYYIGLSQTGTYDASLLPLHV
                     KAISDPETQKNDPSARQAYYNFICREQIAKRDYRQTKYMSLFSKAAYVPEWMAKRNYT
                     VGNTRRAFRMVSMPYAQIADSTIVVNDKDLETYYNKNKNKYKQTEGRTVEYVSWDFIP
                     SPADSAATVAALMESVAKMKTAKSDSAFIATRSEDPDRFGNSNYSRQDLYTQGIDSAY
                     VDSFFVRPAGSLIGPFQTGEYYKVAALKSRTMMPDSVKSRHILVGISEQRDSIAAKKI
                     VDSLMGLLNAGGDFTKIAGESSDDQSSAQQGGDLGWTTPSINFVPEIKDYLFKTGSLG
                     KVSVVKSMYGYHIIEITEMHNKKEFVNVAFLSKSIGPGKETVSSIEKMADDFYAAHQD
                     PESFEQGVIDEKLFKRTTQPLTKSMFEVPGLPNSREIITWAYESDLNEFKYFNMTDRV
                     VVAYLKEIRVNGVADLEYVRDQVEQEVILEKKAEILTKKINEAMGSGATMESIASKLG
                     VRVDSVLNASMATPSAPILGREPKVIGAVFAAEQGKITAPISGLRGVYVAQVFQETPA
                     PEQTDFTMNKNQLTYSMQNKFQGQGLLIVLKERANVVDNRYIYGD"
     gene            complement(5359..5655)
                     /locus_tag="PLM90_10130"
     CDS             complement(5359..5655)
                     /locus_tag="PLM90_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89776.1"
                     /translation="MAKKEAPNMYVRLMKIFEVMWLVTAAVALVMAITRALNGQQYGN
                     YIYITLFTGSVAYFMFRFKKRNRLYLEEHYRKQEEMAKKQAGASKSGETEAPKA"
     gene            5730..6470
                     /locus_tag="PLM90_10135"
     CDS             5730..6470
                     /locus_tag="PLM90_10135"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HMZ89777.1"
                     /translation="MQLVLDLGNTKHKYAIYEGNELVRLGSEPQLTAEITANLIDIYG
                     IDQAIVSSVDHFDEALESLLEERTGLLQFTHTTPLPIINLYGTPATLGKDRLAGAVGA
                     HTAFKGSPVLVVDAGTCIKYDFLDKEGIYYGGAISPGLFMRFKAMHNFTARLPLIDPE
                     NYATVLEVPLTGTSTVDSLRSGALNGALFEVEGAISAYTGQHAGLAVILTGGDAGFFE
                     LHMKSRIFARPNLVLEGLHTILNYNLNH"
     gene            6470..7864
                     /locus_tag="PLM90_10140"
     CDS             6470..7864
                     /locus_tag="PLM90_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89778.1"
                     /translation="MHLRILPLLALTTLTLSISAQVNSPYSRYGLGNMYPGSFTAANG
                     MGGFSAANFSPSAINFLNPASFAEIGGASFDVGVYGTVMSLNTNSETFTSGDANLSNM
                     AFAFPVYKNLRRDRFGLSFGLVPFSTFQYDILDETDTGDPDLGTKQYNYVGDGQLYQL
                     YGGFGYRHQTDTVSQVKSIQGRQDTVISAGFLSIGASSALLFGSLYNTTYASFPDILN
                     SQTTKLSRDNDVNGGMYNIGVGYQKQYVRKHGTKRDILNWKFGGTFSPQVDVHGTQSI
                     LWTNILKYGNYETVTDTLYTAPDTSGNIHMPMSYSAGLSFSFFSSDDENKNQFTLGLQ
                     YASTMWSQYDGFQDAGELADSWRATLGAEFIPKKKQDQNAPLMYRFGAYTGKSNLIID
                     GVQLNDVGFSAGLAIPMGYGENVLPYYRNSKINMFLNVGQRGANADISETYFNFGVGF
                     SMIDLGWFMKYKLN"
     gene            7888..8445
                     /gene="lptC"
                     /locus_tag="PLM90_10145"
     CDS             7888..8445
                     /gene="lptC"
                     /locus_tag="PLM90_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMZ89779.1"
                     /translation="MITSTWMSKYPLTLLVAMTIFAGCVNNVNEVNKATQLAEPGIEY
                     GKDIELFFSEDGEVKIRVTAPTVVRHNTEDPYTEFTDGLKVEFYNDSMHVDTWLTANS
                     GTRYEAQGRTVLQNDVQVLNKKNEHLSTEELIWDEKRHIIYSDKFVKITTPDQVLYGD
                     GMEADEQLTRYTIKKPRGTIQAEME"
     gene            complement(8529..8843)
                     /locus_tag="PLM90_10150"
     CDS             complement(8529..8843)
                     /locus_tag="PLM90_10150"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HMZ89780.1"
                     /translation="MLRTLIVLAGLLFFISNADAQIIYKTDWKSEAAKKVYVTDMPAE
                     ADIVVFKTEWKSDATEDSGQWYFTEWKDEADLIVYFTEWRSEADLVIYYTEYKEEAGK
                     RK"
     gene            8952..9296
                     /gene="gldC"
                     /locus_tag="PLM90_10155"
     CDS             8952..9296
                     /gene="gldC"
                     /locus_tag="PLM90_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HMZ89781.1"
                     /translation="MGKTSTIKLNVTLGGDNFPEEITWSADDSGQKAAESCKAFMLSI
                     FDGNADESLRIDLWTNEMRQDEMDKFFFETFATMADTYKRANQQEEVAKFIRDFAFSF
                     GEKVGLLKRKES"
     gene            9349..10128
                     /locus_tag="PLM90_10160"
     CDS             9349..10128
                     /locus_tag="PLM90_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ89782.1"
                     /translation="MHDKLRIGICLSGGGARGVAHIGVLQALESAGIIPYAVSGCSAG
                     AMVGALYCAGLPPRQIFSIIEKKSIYSIIKMGLPDKGMMELAYFKKVLSENIAHDSFE
                     LLQKPFFISVTNLNTGAAEIIGNGKLIEPVIASQSIPLVFKPQRIGEHLYVDGGVLNN
                     LPIEPLQEICDIIIGVNVTPVRYAENISGMRDVGYRVLNLTLRQNMAPRLAQCDFVIE
                     PDTGGYTIFDITKSKTIYDAGYEAALPVAEEIANKHFFNTK"
     gene            complement(10238..>10505)
                     /locus_tag="PLM90_10165"
     CDS             complement(10238..>10505)
                     /locus_tag="PLM90_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89783.1"
                     /translation="DEGLGLNFYSNGISIYPNPTTGFLSIELNDLKTGDFELYSSSGS
                     LMEIGDISSKITIIDLQSVPAGIYHLIYKTDTEYSVFRIVKVIE"
BASE COUNT         2986 a         2349 c         2166 g         3004 t
ORIGIN      
        1 gcataataca tgtgaataaa tccaaagaaa ataacaactg ccactaacaa tgctgcgcgc
       61 ctgaaagtat aattcttttt ctccagtcgt atcatcagcc accatatcag cggcacaaat
      121 aacgcataac tcaatgcaaa atgcccccag aatctatcga gctgtggcgt catcaaccca
      181 atgagcaaag ccataccggc agcataccag gcaggcatgc gataatgccg caaaatgtga
      241 aatatcaata acacgcccgc ccataatgaa atgaacatca ataaatgcaa aatggcaacc
      301 gtattatcat gtacgggaaa cacatgatca tcaatcacat tgatcaacca ggaaatcatt
      361 ggctgatcat cggtgaatac cacatgctca ccataagggt aattcattcc cgtaaaacga
      421 tcacccttat cgttggcaac gtaccaagcc ggtgtataat aattcttgaa cgcatcgccg
      481 ccggtggtga gtaaataatg attgggagaa aaaatgattt tttgccacgc cgcacaaatg
      541 atcactgccg taaccaatgc cagtatccat ccgggagcgt tgcgtttcat catcgtgtaa
      601 agatgcttcg aaataaaatt ttcagaaagt ttcccgattc ctgtaataat atgctcatgc
      661 gcatactgct gaattgcaca ttatcgcgta gtgctatctc aacaggcttt attttcaacg
      721 atttatttct tgatgccaga aaaataaatt ccagatcgaa taaatatctg tcaatgctcg
      781 tacgcataaa catattcctt cccctgtcat taaatccctt taaaccacat tgtgtatccg
      841 acacttttaa tcgcaatacc gttgttgtac atgcgcgcaa taattttgaa ataaatcgcc
      901 tgaaagcagg cacatgtgca taatatgtcg cgtcttttac tccggccgca acatctgctt
      961 ctttattggc gagcacggta tataattgga taaagctcgc agtcgtatac ggaaaatcca
     1021 catcggtata aatggtaata tccgcgtttg atgctgctac accttgtcgc aacgcataac
     1081 cctttcccct gtttttttga tagctgataa attcaaattt agcgatcgcc gatttcaatt
     1141 gcagcaaatc ttcatcacca atgctgcgcg aactgccatc gttaatcaaa attacacgaa
     1201 tatcggcatg tggaatcaca gcagctattt caccatatcc cgtaataata tggtccaccc
     1261 atgaaggagc gggattgtaa cacggaagga tgatatcgag cgtgggttgc gacacttatc
     1321 gggttggaca attcaaaatt tcatgtaacg cgatttacgg gcataacaat accctatacc
     1381 gcaggaataa gtttcggtaa aactaatgaa ataatctgtt gagatttccc cgatcccgct
     1441 atcaggcagg tatcttaatg cgatattgct gtccggcttt aacggtaagc atatcggacc
     1501 ttaaccaagg attataatat ttcaacatct tgtaattcgt gccattcttt ttggcaaatt
     1561 ccgacaggtt cggaattgat gtggtaactg taacctcctt atactgcaat gggtcgtaca
     1621 agtcctcctt ggtcagaaaa aatccataat cctgtgtatg ctcataaata atcttaaatg
     1681 ccaaagccct gaacacatag cgcgatgttt cttcacggaa atataaatcg tagtaggatt
     1741 tttcatcctg ataattaacc gcgccttcta ttgctcccat accggcattg tacgaagccg
     1801 ctgccagggt ccagttattg aatttcgcat aggcatcctt aaaatactgc gaagccgcct
     1861 cacatgattt ctccatatca taacgttcat ccacttcacc attaatggtc aatccataat
     1921 gctcaccggt attttccaaa aactgccaat agccatgagc acctgaggga gatatcacat
     1981 tgcgcaatcc gctttctgcc attgcaagat atttaaaatc ttcaggcact ccgtttttct
     2041 taagggtaga ttcaataatc ggaaaccaac gatttgccag tttcagattt tgaatggtat
     2101 tggaatgcca atatcccgta actgtcaatt cgcgatccaa acgttcacgc acttccggat
     2161 catcgagcgg cacttcttca ccggcaaagg tgagcgtctt cggcaatttt gctgcaaaat
     2221 atcggatagt gttcacctca tcggcaagtg aacgcaaggt gtctgtaggc atggcagatt
     2281 ctacatgcgc gccattgcca tttgcatttc cctttaccgc caatgcaact ataaggccta
     2341 cgcctatcac ggcaccaagg atgatataat gtactttttt cacgcttgtt cttttttctt
     2401 gaataccggc accgtagagc atggctcgcc atacatgatg cttttagtta cagggcgcaa
     2461 taaggctgtt atttcgagat aggcttcagc aggaacgggt ttgtcgctgc atccttttat
     2521 cactacgcgt tgatcgcgat attgttctaa atctacttgc gataactgct gtgtgtaata
     2581 acgtttcata gccagttccg gatctcccat tactaccatt tctgcaatac cggcaacatt
     2641 tgcagccacg agcatccagg cccaatttgg tatgatggcg tctgcactgc aataaacggc
     2701 aactgttttt ccggcatact gctgccagtc gatagcggcc aatgcttcgc gaaaatcctt
     2761 ttctcgcaga atgagaccgc gaaacaaata atctttcaga tcaaaagaca cgatgcttcc
     2821 ttctgcaggg aaaaggtctt ccaggtcgaa ggtgagtaaa ccgctttgag ctactttatt
     2881 tactagttcc atgtccggac tttttagaca acaataaaat tacatcattt cgcactcccg
     2941 aaaaagcgaa cccgcaagca gtgcaatcgt aagcaccgga tgcgggtttg cgtatcggta
     3001 ccggaagagt aacgtcgtcc ggcggaaaat agtttagtcg ccgtagatat atctgttatc
     3061 taccacgttg gcacgctctt tcagcacaat caataatccc tgtccctgaa atttattttg
     3121 catggaatag gtaagctgat ttttattcat ggtgaagtca gtttgttcag gtgcaggtgt
     3181 ttcctggaat acctgagcaa catatacgcc gcgcaatcct gaaatcggag cggttatttt
     3241 tccttgttct gcagcaaata ctgcacctat cacttttggt tcgcgaccga gaattggtgc
     3301 acttggtgtt gccatagatg cattgagtac tgagtcaaca cgtacaccca gtttagatgc
     3361 aatagattcc attgttgcac cactgcccat tgcttcattt attttcttcg tcaatatctc
     3421 agcttttttc tcaaggatca cttcttgctc aacctggtcg cgcacatatt caagatctgc
     3481 aacgccgttc acgcgaattt ctttgaggta tgcaacaacc acgcgatcgg tcatattgaa
     3541 atatttgaat tcattcaaat ctgattcata tgcccaggta atgatctcgc gtgaatttgg
     3601 caaacccgga acttcgaaca tgcttttggt aagcggctgt gttgtgcgtt taaataattt
     3661 ttcgtcgatc acaccttgtt caaatgattc aggatcctga tgtgcggcat aaaaatcatc
     3721 agccattttt tcgatggaag aaacagtttc tttgccggga ccaatagatt tactcaggaa
     3781 cgcaacattt acaaattctt ttttattgtg catttctgtg atttcaataa tatgatatcc
     3841 atacatcgat ttcacaacac tcacttttcc taatgagcct gttttaaata aataatcttt
     3901 tatctcagga acaaaattga tagacggggt tgtccaaccc agatctccgc cttgttgcgc
     3961 gctgctttga tcatctgaag attcaccggc aattttagtg aaatcaccac cggcgtttaa
     4021 taatcccatg aggctatcta ctatcttttt cgctgcaatg ctatcgcgct gctcgctgat
     4081 gcctacaagt atgtgacgcg attttactga gtcaggcatc atagtgcggc tctttaatgc
     4141 agccacttta taatactcac ctgtttggaa tggaccgatg agtgaaccgg caggacgcac
     4201 aaagaaagaa tctacgtatg ccgaatcaat accttgtgta taaagatctt gtctgctgta
     4261 attgctattg ccaaaacgat caggatcttc actgcgtgta gcaatgaatg cactgtcgct
     4321 ctttgctgtt ttcattttcg caacgctttc cattaatgcg gcaaccgtag ctgcgctatc
     4381 tgcgggagat ggaataaaat cccagctgac atattctaca gtgcgacctt ctgtttgctt
     4441 gtacttattt ttattcttgt tgtaatatgt ttcgaggtct ttatcattta caacaattgt
     4501 tgaatctgca atttgtgcat aaggcattga caccatgcgg aaggcacgac gtgtattgcc
     4561 aacggtataa ttgcgtttcg ccatccattc aggaacatat gctgcttttg aaaacagcga
     4621 catatatttt gtttgacgat aatcgcgttt tgcaatttgt tcgcggcaga tgaaattata
     4681 atatgcctga cgagcagaag gatcgttttt ttgtgtttca ggatcgctga ttgctttcac
     4741 gtgcaatggc aataatgatg catcataagt accggtttgt gaaagtccga tgtaataatt
     4801 ttttatatac ggatgcggat ctggaccata aattaaattc gtcaattctt cttcggtaac
     4861 gccaagacct aattcttcaa gttgcttgtc gatgattttc tcattcacat attcagtcca
     4921 tgattgttca ttgagaccaa attttgtctg agggtctaac tgcatagaac cgttttcata
     4981 ttgcaacacc tgaccggttt ggagggtgag atattccaat aatgttctgc cgcttctttc
     5041 ggcaacttca tcacggttta cttcttcacc atacatggtt gcataatccg gggctttatt
     5101 tccacggaac atacctgtgt ttgaattagt ggcatcctgc accagaaagc caatcaaaca
     5161 caatgcaata aggccaactg cgagccacga atactgacgg atttttgaaa taagcatatg
     5221 ctattcgaat aatattagaa tattggggat gcaaaggtag tggatggcgt gtggaaaaca
     5281 aaactatcgc ccgtggtata gcactttcaa cctcaaaaag ggggtaaaat gtgccggtac
     5341 gcctgaaaaa cactaaaatc aggctttggg agcctctgtt tcgccggatt tggaagcccc
     5401 ggcctgcttt ttggccattt cctcctgttt gcgatagtgt tcttccaaat acagtctgtt
     5461 tctttttttg aagcggaaca tgaagtaggc caccgaaccg gtaaacaacg taatatagat
     5521 gtagttgccg tattgctgac cgttcaacgc tcttgtaatg gccattacca gtgccacggc
     5581 agccgtcacc agccacatca cctcaaagat cttcatgagg cgcacataca tattgggagc
     5641 ttccttcttc gccataataa ttcgtcaaaa ttacgtcttg ttagcggatt tcccctgtcg
     5701 ccatgcttcc tgattaatat cttcgcacca tgcaattggt actggatctc ggcaatacga
     5761 agcataaata tgccatttat gaaggcaatg agctcgtaag gctgggcagc gaaccgcaat
     5821 tgactgcgga aataacggca aacctcatcg atatctatgg tatagatcaa gccattgtga
     5881 gcagtgtaga ccatttcgat gaagccctgg aatccttgct ggaagagcgt accggcctgc
     5941 ttcagttcac gcataccacc cctttaccca tcatcaatct ctacggcacc cccgcaaccc
     6001 tgggaaaaga cagacttgcc ggagcagttg gcgcacatac cgccttcaaa ggctctcctg
     6061 ttttggtggt ggatgccggc acctgtatca aatatgattt tctggataag gaaggtatct
     6121 actacggcgg agccatttct cccggccttt tcatgcgatt caaggctatg cacaatttta
     6181 cggcgcgact gccgttaata gacccggaaa actacgcaac cgtgttagaa gtgccgctga
     6241 cagggacttc tacggtcgac tcgctgcggt caggagccct gaacggagca ttgtttgagg
     6301 tggaaggtgc tatcagcgcc tacacaggac aacatgcggg attagcagtg atacttaccg
     6361 gcggagatgc gggtttcttt gaattgcata tgaaaagcag gatatttgca cgcccaaatc
     6421 ttgtgctgga aggcctgcac accattttga attacaatct gaatcactga tgcacctccg
     6481 aatactaccg ctgttagccc ttacaacact gaccctctcc atttctgcac aagtaaattc
     6541 cccttactca cgatacggtc tcggtaatat gtacccggga agttttaccg ctgcaaacgg
     6601 catgggcgga ttttctgcag ccaatttctc tccaagcgca attaattttc tcaacccggc
     6661 ttcatttgca gaaatcggtg gcgcctcttt cgatgttggc gtatatggca cggtgatgtc
     6721 actcaacaca aattccgaaa catttacttc cggcgatgca aacttgtcaa atatggcatt
     6781 cgcatttccg gtatataaaa atttacgtcg cgacagattt ggcttaagct ttggattagt
     6841 gcccttcagc accttccaat atgatatcct cgacgaaaca gataccggcg atccggattt
     6901 gggaaccaaa caatataatt atgttggcga cggacaatta tatcaacttt acggaggctt
     6961 cggttatcgt catcaaacag atactgtttc tcaggtaaaa agtattcaag gccgacaaga
     7021 tactgttatc tcggcaggat ttctgagcat tggcgccagc tccgcattat tgttcggctc
     7081 attatataat actacttatg cttcgtttcc cgatatcctg aattcgcaga caacaaaatt
     7141 gtcgcgcgac aatgatgtga acggcggcat gtataatatt ggtgttggat atcaaaaaca
     7201 atatgtgcgc aaacatggca caaaacgcga tatcttaaat tggaaattcg gcggtacttt
     7261 ttcaccacaa gttgatgtac atggtacgca atctatcttg tggaccaata ttttgaaata
     7321 tggtaattac gaaactgtaa ccgacacttt atatacggca ccggatacat caggaaatat
     7381 tcatatgcct atgtcttatt ctgccggttt atcctttagt ttcttcagta gtgatgatga
     7441 aaataaaaat caattcaccc tgggattgca atacgcttct accatgtgga gtcaatatga
     7501 cggatttcag gatgcaggtg aattagcgga tagctggcgc gctacattgg gtgcagaatt
     7561 tattcctaaa aagaagcaag atcaaaatgc gccactgatg tatcgcttcg gcgcatatac
     7621 aggtaaaagc aatctgatta tcgacggtgt gcaattaaat gatgttggat tttctgccgg
     7681 acttgccatt cctatgggct atggcgaaaa cgtgttgcca tattatcgca attctaaaat
     7741 taatatgttc ctgaatgtgg gacagcgcgg agcgaatgcc gatatcagtg aaacatattt
     7801 caacttcggc gtaggcttca gcatgataga ccttggttgg tttatgaaat ataagctgaa
     7861 ctgacgcata tagcgtaaat ttatcctgtg atcacttcaa catggatgtc gaaatacccg
     7921 cttactttat tagtggcgat gacgattttt gccggctgtg taaataatgt aaatgaggtg
     7981 aataaagcca cgcaactggc agagccggga attgaatacg gcaaagacat cgaattattt
     8041 ttcagcgaag atggcgaagt gaaaattcgc gttacagcac ctactgttgt gcggcacaat
     8101 accgaagacc cttacaccga atttaccgat ggccttaaag ttgaatttta caacgacagc
     8161 atgcatgttg acacctggct gacggcaaac tcgggtacac ggtacgaagc acaaggaaga
     8221 actgttttac aaaatgatgt gcaggtgttg aataaaaaaa atgagcatct gagcacagaa
     8281 gaattaatct gggatgaaaa acgccatatc atttactccg ataaatttgt gaaaatcacc
     8341 actcccgatc aggtattata tggcgatggc atggaagccg atgaacaact cacgcgctac
     8401 accatcaaaa aacccagagg gacgattcag gcggaaatgg agtgattaat tagatgatgc
     8461 aagtgtgcag caacttttgg gccacctctg cgcctctgcg cctctgcgtg aaaaaccctc
     8521 cgcaccacct atttccgctt ccctgcttct tccttgtatt ctgtatagta gatcacaagg
     8581 tcagcttcag agcgccattc tgtaaaatac acgatcaaat cggcttcatc tttccattcg
     8641 gtgaaatacc attgtccgct gtcttccgtt gcgtcgcttt tccattcggt tttaaacacc
     8701 acaatatctg cttcagcagg catatcggtt acatacactt ttttggccgc ttcactcttc
     8761 cagtcagttt tataaataat ctgagcatca gcattgctga taaaaaacag aagtcctgcc
     8821 aatacgatta atgtgcgtag catttcgggt ttaggtttgg ttaaagttcg tcagttttgg
     8881 gcatctcgcc aaatagcaaa gagccccata ccatcatctt tgactaattt cgtgccaaaa
     8941 tagcaacaac catgggtaaa acctcaacta taaagctcaa tgtgacctta ggcggcgata
     9001 attttcctga agaaatcacc tggagtgccg atgacagcgg tcaaaaggct gccgaaagct
     9061 gcaaggcctt catgctcagt atttttgatg gcaatgccga tgaatcgctg cgcatagacc
     9121 tatggacgaa tgaaatgcgc caggatgaaa tggataaatt tttcttcgaa acctttgcaa
     9181 ccatggccga tacttacaaa agggctaatc aacaagaaga ggtagcaaaa ttcatccgcg
     9241 attttgcatt ttcattcgga gaaaaagttg gattgctaaa acgcaaggaa tcctgaattt
     9301 ttttagtgta tataccgttt acaatgcaga tacagtatat ttactgatat gcatgataaa
     9361 ctgcgcatag gtatttgttt gtcgggaggc ggcgccagag gtgttgcaca tattggcgta
     9421 ctgcaggcat tagaaagtgc gggtattatt ccatacgccg taagcgggtg cagcgcaggt
     9481 gcaatggtgg gtgcactgta ttgtgccgga ttgccaccgc gacaaatttt cagcatcatc
     9541 gaaaagaaaa gcatttacag catcattaaa atgggactgc cggacaaggg catgatggaa
     9601 cttgcgtatt tcaaaaaagt gctttcagaa aatattgcgc acgacagttt tgaattattg
     9661 caaaagccat tttttatttc cgttaccaat ctcaataccg gcgccgcaga aattattggc
     9721 aatggcaaat tgatagaacc cgtaattgca tcgcaatcta taccactcgt atttaaacca
     9781 cagcgcatag gagaacattt atatgtcgat ggcggtgtac tcaataattt accgatagag
     9841 cccctgcagg aaatttgcga tatcattatt ggtgtgaatg taacaccggt tcgctatgca
     9901 gaaaatatca gcggcatgcg cgatgtgggt taccgtgtgt tgaatctcac attgaggcaa
     9961 aatatggcac cgcgacttgc acaatgcgat ttcgttatcg aaccggatac gggaggctat
    10021 actattttcg acatcaccaa atcaaaaaca atttatgatg cgggatatga agcggcattg
    10081 cctgtggcgg aggagatagc gaacaagcat ttttttaata caaagtagct catcaacata
    10141 acttttgatg gagctccggt tttattagac caaactaaaa aagtttaaat taattcctag
    10201 tgtccaatta attgggtaat gcgtactagc taaaaaacta ctcaataact ttgacaattc
    10261 tgaaaactga atattcggta tcagttttat agatcaaatg atatatacct gccggtactg
    10321 attgcaagtc aataattgta atctttgagg aaatatctcc tatttccatt agagagccgc
    10381 tagagctata caactcaaaa tcaccggttt ttaaatcatt taattcaatt gacaaaaaac
    10441 ctgtagttgg gttcggataa atagatattc cattcgaata gaaattcaag cctaatcctt
    10501 cgtca
//
LOCUS       DAOQOU010000115        10412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3350_length_10412_cov_5.202665, whole genome shotgun sequence.
ACCESSION   DAOQOU010000115 DAOQOU010000000
VERSION     DAOQOU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_10412_cov_5.202665"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1099
                     /locus_tag="PLM90_10170"
     CDS             179..1099
                     /locus_tag="PLM90_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ89784.1"
                     /translation="MRILLFSLLCSMMLAGSVQAQDTLKQGLIQVNDTHLFVKIMGKG
                     DTILVIHGGPGLNHGYLEPHLLPLAKTHTLIFYDQRSSGESQLSVKSHMNFATFADDI
                     EAIRKHFNINHLNLLAHSWGCLVAATYMLNYPEHVQSVVFSNPVPFDHTMAEMSAQTA
                     ADRETPADSIHKAQLIASDAFRNGDVEPVKELMKTSFKLLFCDTTNLSLLDPSLPSSY
                     MVASLSLYGFMDDMKNYNFFPKMKDIPIPALIIHGNCDISPLDADLRLQKCFTNADVT
                     IFENSGHFPFIEENKKFNKTVSKFFLDHAK"
     gene            1354..2667
                     /locus_tag="PLM90_10175"
     CDS             1354..2667
                     /locus_tag="PLM90_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HMZ89785.1"
                     /translation="MQRLPALLLLIACTHTVVIHAQFCNDTRFTEAEYFSDSEIETNY
                     NVSYGLASQWFMDLPQPSLNTLDIAFPDQTVDPMEKRPLIVLVHGGGFWGGEKESFSY
                     HLQELAKNGYVVAAINYRKGWMGSPLDCDGDPNSLSLAIYRGMQDTHAAIRFLVANAD
                     VYGIDTANIFAGGESAGVYAVMNAYYLTQDDWESYNPGYGYLYGDINASTNEYTTTFK
                     LKGILNMWGGMVDTAFVSVNEAVPSIAFYGMSDGVIPPGHGNIQYCENFAEIFGALGL
                     SEFYNNNNICNVLHQNPTEGHEAYPADYVCPNIACFIKGVLCNTCSTELMHYEISDCS
                     ADVVIDTTTDTTETLNIEIAQIETLSLFPNPADQRIQCKIDNTWPTDASITVYDLAGN
                     FMQIAVGQAGLVRYADVSSLPEGVYLLRIQAANRVGSGSFVVARH"
     gene            2763..3431
                     /locus_tag="PLM90_10180"
     CDS             2763..3431
                     /locus_tag="PLM90_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ89786.1"
                     /translation="MSKQVHILIAETQYLIIEGLQSIFRRYPEFSVLGIMVNKQDLFD
                     NLHVLKPDVVVIDPLSLEDFRVDDLQTIETVCPGARALIITDISDPNIVHRIVDTGAK
                     GYLTKTCDEKEIVDAIYALLRGEQMYCHKVVNILVDGRTVEVDNCEPTGLSEREVEII
                     RLIANGYTTRQIADTLYRSFHTITTHRKNIMKKLGINSTSELMVYAMNTGLIQPREQL
                     VAGK"
     gene            3479..4741
                     /locus_tag="PLM90_10185"
     CDS             3479..4741
                     /locus_tag="PLM90_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ89787.1"
                     /translation="MNTTTIRWVIALGIVAVVTIILSQVFWIRKGLIINQSNFENAVT
                     VTLEQIAAKIEIANHGKKVTAHPVVRISPHAYKVNIDEPIDLNQLDYFIRSEFANPFH
                     KVDFIYQVFESTRDDLVFEETVSANSSESTIVFPTNLPKLMESTYYFKINFPERPIVN
                     SVMLFVWATSIIAMAVVIAFFWYSLDVIFRQQRLSKFQKNFINNLAHEFKTPISTIGI
                     SADVLLEPEIAQEPDRLKGYANIIRNENLRLTGQVSKILELAKLEDDELLLNLETFHI
                     NPLIEEIVQSFTVRVEEKSGGIVVDYTTKQDLVTADRVHLQNVVNTILDNALKYNDKI
                     PEITITVGRDQQGLFINIGDNGIGIPKEFHRRIFEKFFRVPTGNVHNVKGYGLGLNYV
                     KMIAKSHGWHLRVDSQIGKGSIFTVIIP"
     gene            4751..5458
                     /locus_tag="PLM90_10190"
     CDS             4751..5458
                     /locus_tag="PLM90_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ89788.1"
                     /translation="MDSRKPKILYVEDDVNLGFVTKDNLLKRGYDITLCEDGLTASHT
                     FQKQKFDLVILDVMLPKIDGFALAQKLRDKDKNVPIIFLTAKSMLEDKLQGFKLGADD
                     YITKPFSIDELTHKIEVFLKRSKVFAESPEAESKNIYHIGTYTFDFKELKLFNTTQQH
                     TLTIREAELLRMLSIQRNQVVRREAILHQVWGEDNYYIGRSLDVFISRLRKLFADDPG
                     VEIENVRGVGFKFNVAD"
     gene            complement(5569..6906)
                     /gene="gdhA"
                     /locus_tag="PLM90_10195"
                     /pseudo
     CDS             complement(5569..6906)
                     /gene="gdhA"
                     /locus_tag="PLM90_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
     gene            complement(6990..7442)
                     /locus_tag="PLM90_10200"
     CDS             complement(6990..7442)
                     /locus_tag="PLM90_10200"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HMZ89789.1"
                     /translation="MSLQFSTYDLRVQESESGRRIYDIIRKKYVVLTPEEWVRQHIIH
                     YLITDHGFPKSLISVEKQLTVNTLTRRTDIVLYNHAGKPVLIVECKASDIKLSQAAVD
                     QAARYNLTLQVPWLWVTNGVSHVWCHISHEEGNWRVEGHLPPVSELLR"
     gene            7470..8480
                     /gene="holA"
                     /locus_tag="PLM90_10205"
     CDS             7470..8480
                     /gene="holA"
                     /locus_tag="PLM90_10205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMZ89790.1"
                     /translation="MDYRDLINDIKARKFKPVYLLHGDEAYYIDKIAEYMEDNILPPD
                     QKDFNFSVFYGKDTHPQTVIDTSMRFPMFSDFNVVMLKEAQMMKGKGNEIDKLETYIE
                     KASPTTILIICYKEGRFDARKRMFKLIKEKGVIFESETIKESELPMFIESYLKRKKAS
                     IDSAALRLLVQYLGADLAKMSNELDKLCINLPESGRITVDQIEKNIGISKDYNVYELQ
                     SALLTKNFALVYTIVSYINDNQKANPFIFTISNIHAAFQRLYHYLLGGEVSDFDMYKV
                     YQIHSSQKNEYKAARRLYTVQRVEEIFELLLDYDLRSKGLNNGETEHDELLREMVYKI
                     MS"
     gene            complement(8496..9431)
                     /locus_tag="PLM90_10210"
     CDS             complement(8496..9431)
                     /locus_tag="PLM90_10210"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HMZ89791.1"
                     /translation="MLVIRNLQSLPDLTGCVVTIGTFDGVHHGHCAIIDRITEEAKKR
                     NGKSVIITFYPHPRQIITPGSPVYHLNTLEEKLSKLQARGVDVVVVVPFSREFSDISA
                     EDYVRTFLIEKFHPAVIVFGYDHKFGKNRTGDIHLLKSIAGGYAIHVEEIPAHIIDSL
                     TVSSSKIRAFLLEGNIDSANELLGYNYALTGPVVKGDQIGRTLGFPTANIFVEDSYKL
                     IPANGVYVVRVVMKQHPGEYFGLLSIGTRPTFNKTESRVEVHILDFNESIYGETITIQ
                     FLHFLRKDKKFDSATSLVEAMHADKQQALTYLASL"
     gene            complement(9456..10178)
                     /gene="truB"
                     /locus_tag="PLM90_10215"
     CDS             complement(9456..10178)
                     /gene="truB"
                     /locus_tag="PLM90_10215"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HMZ89792.1"
                     /translation="MLFTPEEIAEGKFLLLNKPLQWTSHDVIARLRGPLKTFTGNKKL
                     KVGHAGTLDPLASGLLIVLTGKATKRADETQNLDKEYTGIITLGGVTASYDLETPVES
                     ITVTEHITDLLVQQAAQHFTGAFMQLPPAHSSIKIQGKPAYTYAREGEEMKMRPRPIE
                     IFQFEIIAFRNPHIDFRVHCSKGTYIRSLAHDVGQFLGCGGYLSALCRTKIGNYHLEN
                     AYTLDTVIDFLRQSNQHLHQSQ"
     gene            complement(10181..>10412)
                     /locus_tag="PLM90_10220"
     CDS             complement(10181..>10412)
                     /locus_tag="PLM90_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMZ89793.1"
                     /translation="GANKGLHPYELLLNNHDNMTAFIIGNVIAFIIAILAMRSFVGYL
                     TKHGFKIFGYYRIIVGAIILILIAAGVDMQIL"
BASE COUNT         2973 a         2152 c         2163 g         3124 t
ORIGIN      
        1 aagtatgatg gcgtgcacca tgatgctgcg tgccggaatt aagcatgttg tcaacgtagc
       61 cggcggtatc aatgccattt tgaaggaaaa acctgatctg gtgcaatatt tcgagttggt
      121 gtgataacac ttatcgtata actttacatc cctgcccata acccggcagg gattttttat
      181 gcgcatactc ctcttttcgc tgctctgctc catgatgcta gcagggtctg ttcaagctca
      241 ggataccctc aaacaaggtc ttattcaagt caatgacact cacctgtttg tcaaaataat
      301 gggaaaaggg gataccatac tggttattca tggaggtccg ggcttgaacc atggatatct
      361 tgaaccgcat ttgctgccat tagcaaaaac gcataccctc attttctatg atcagcgctc
      421 ttcaggagaa tcgcaattaa gtgttaaatc gcatatgaat tttgccactt ttgccgatga
      481 tattgaggct atcagaaagc actttaatat caatcatctc aaccttctgg cgcattcctg
      541 gggatgtctt gtggcggcaa cctatatgtt gaattatcct gaacacgtgc aatcggttgt
      601 tttcagcaat cccgtgcctt tcgatcatac catggcagaa atgtcggcac agaccgctgc
      661 tgatcgggaa acgccggccg atagtatcca taaggctcaa ttaattgcaa gtgatgcctt
      721 ccgaaatggc gatgttgaac cggttaaaga attaatgaaa acctctttta aactcctttt
      781 ttgcgatacc accaacctga gcttgctgga tccttcgttg ccttcctcct acatggtggc
      841 ctcactttcg ctttatggtt tcatggacga catgaaaaac tataacttct ttcccaaaat
      901 gaaggacatc ccgataccgg cattaatcat ccatggtaat tgcgatatca gccctctgga
      961 tgctgatctc cgtctgcaaa aatgttttac caatgccgat gtcactattt tcgagaattc
     1021 aggacatttt ccgttcatcg aagagaacaa aaagttcaac aaaacagtgt cgaagttctt
     1081 tttagatcac gcgaaatgag gaatttcctc atctgtgtta tctgactccc tagtctcgca
     1141 gcatgtatac gatttagttt aaattattgc caagtttagc tttcggtaca tcatcaaaat
     1201 tgaactgttc gacggcaaat ttgttgtaac agttaccatg tcatgaaaac cttacttcag
     1261 tagcaattgt tcttactgtc ttccgtattt ttgttattcg ttcttataaa accatacaca
     1321 cacacgacta tggtttaaca ttttaacatt tgtatgcaga gattacccgc actcctcctc
     1381 ctcatagcat gcacacacac agtagtaata cacgcccaat tttgtaacga cacccgtttt
     1441 acggaagccg aatacttctc cgactccgaa atcgaaacaa attataatgt ttcgtacggt
     1501 cttgcgagcc agtggttcat ggatttgcct caaccaagtc tcaacacatt agatattgca
     1561 tttcccgatc agactgttga cccgatggaa aaacgtccgc ttattgtgtt ggtgcacggt
     1621 ggtggctttt ggggtggtga aaaagaatct ttttcctatc atcttcaaga actggcaaag
     1681 aatggctatg ttgttgcagc aataaattac agaaaaggtt ggatgggttc accgcttgat
     1741 tgcgatggag atcctaactc attgtcgctc gccatttatc gcggtatgca agatacccac
     1801 gccgccatac gttttcttgt tgctaatgct gatgtgtatg gcatcgatac tgcaaatatt
     1861 tttgcgggag gtgaaagtgc cggagtttac gcagtaatga acgcttatta tttgacgcag
     1921 gacgattggg aaagctataa tcccggttat ggttatttat atggcgatat caatgcgtca
     1981 acaaatgaat ataccactac atttaaattg aaaggcatcc tcaatatgtg gggcggaatg
     2041 gttgatacag cttttgtttc tgttaatgaa gcagtgccat ccattgcatt ttatggtatg
     2101 tctgatggcg ttattcctcc gggccatggc aatatccaat actgtgaaaa ttttgccgaa
     2161 atatttggtg cactcggact gtcagaattt tataataaca ataatatttg taatgtactg
     2221 catcaaaatc caacagaagg acatgaagct taccctgcag attatgtgtg tccaaatatt
     2281 gcctgcttca taaaaggagt gctttgcaac acatgcagta cggaattaat gcactacgaa
     2341 atttctgatt gttccgccga tgttgtcatt gatactacaa ctgacactac cgaaaccttg
     2401 aatattgaaa tagcgcagat agaaaccctg tcactgttcc caaatcctgc cgatcagcga
     2461 attcaatgca aaattgacaa tacctggccc accgatgctt caatcactgt ttacgatttg
     2521 gcaggcaatt ttatgcagat tgctgtaggt caggccggtt tagtgcgtta tgccgatgtc
     2581 agcagtcttc cggaaggcgt ttacctgctc cgtattcaag ctgcaaaccg cgtaggctca
     2641 ggctccttcg tggtcgcccg gcattgatct cctccccggt cagcacgatc aggatgatgc
     2701 gtgtatgctt gtgattttga acaaaagacg tatctttatg tttagaatta gtctaaatag
     2761 taatgtccaa gcaggtccac attctcatag ccgaaacgca ataccttatc atagagggct
     2821 tgcaatcgat attccgtcgt tatccggaat tttcggtgtt gggtattatg gtgaacaagc
     2881 aggacttatt cgataatctg catgtgttaa aacctgatgt tgttgttatt gatccgctca
     2941 gtcttgaaga ttttcgtgta gatgatctgc agactatcga aaccgtttgt ccgggagcgc
     3001 gtgcattgat catcaccgac atcagcgatc cgaatattgt gcaccgcata gtggataccg
     3061 gtgctaaagg ctatctgacc aagacttgtg atgaaaaaga aattgtcgat gctatttatg
     3121 cgcttttgcg aggcgagcag atgtattgtc acaaagtggt gaatattcta gtagatggta
     3181 gaacagttga agtagataat tgcgagccaa ccggattatc agaacgcgaa gtagagatca
     3241 tcagacttat tgcaaatggc tatacgacgc gccaaatcgc ggatacgttg tatcgcagtt
     3301 ttcataccat caccacgcat cgcaaaaaca tcatgaagaa gctcggcatc aatagtacca
     3361 gtgaattgat ggtgtatgcc atgaataccg gacttattca accgcgtgag caacttgttg
     3421 caggtaaatg accgtcttac acagggaggg ttcaactcct tatctttagc actgatttat
     3481 gaatactact actatccgtt gggtaattgc attaggtatc gtggctgttg ttacgattat
     3541 cctttcgcag gtattctgga tacgcaaggg cttgattatt aatcagagca attttgaaaa
     3601 tgcagttacc gttaccttgg aacagatagc tgcaaaaatt gaaatcgcca atcacggaaa
     3661 aaaggtaacg gcacatcctg ttgtgcggat ttcaccacat gcttacaaag taaatatcga
     3721 tgaacctata gacttgaatc aactggatta ttttattcgt agtgaatttg caaatccatt
     3781 tcataaagtc gatttcattt atcaggtgtt tgaatccacg cgcgacgatt tggtttttga
     3841 agaaacagta tctgccaatt cgagtgaaag tactatcgta ttccctacta atttaccaaa
     3901 gttaatggag tcgacttact attttaagat caacttccct gagcgtccga ttgtgaattc
     3961 agtaatgtta tttgtctggg ccacgtctat catagcgatg gcggttgtta tagcgttttt
     4021 ctggtattcg ctggatgtca tcttccgaca acagcgatta agtaaattcc agaaaaactt
     4081 catcaacaat ctggctcatg aattcaaaac acctatcagc acgattggta tttcggcaga
     4141 tgttttatta gaaccggaga ttgcgcaaga gcccgacaga cttaagggat atgccaatat
     4201 cattcgcaac gaaaatttgc ggcttaccgg ccaggtgagt aaaattcttg aattggcgaa
     4261 attggaggat gatgaattgt tgctcaatct tgaaactttt catatcaatc cattaataga
     4321 agaaattgtg caatcattca cggttcgcgt agaagaaaaa agcggtggta ttgttgttga
     4381 ttacactacc aaacaagatc tggtaaccgc cgaccgagtg catttgcaaa atgttgtgaa
     4441 cacgatactc gacaatgcgt tgaagtacaa tgataagatt ccggaaatca ccattactgt
     4501 tggtcgcgat cagcagggac tatttattaa tatcggtgat aatggaattg gcatcccgaa
     4561 agagtttcac agacgcattt tcgagaaatt ctttagggtg cctacaggaa atgtccacaa
     4621 tgtgaaaggc tacggactcg ggctgaatta tgttaaaatg atagctaaat cacatggatg
     4681 gcacttgcgt gtggattcac aaataggcaa aggcagtata tttacagtca tcattcccta
     4741 actcagacac atggatagca gaaaacccaa aatattatat gtagaagatg atgtcaatct
     4801 cggcttcgta acaaaagata atttgctcaa aagagggtat gacattacat tatgtgaaga
     4861 tggattaacg gcatcacata cctttcaaaa gcaaaaattc gacctggtta ttttggacgt
     4921 gatgttgccc aagatcgatg gatttgcgct tgcgcagaaa ttgcgcgata aggacaagaa
     4981 tgttcctatc atttttctga cagcaaaatc catgcttgaa gataaattgc aaggttttaa
     5041 gctcggtgct gatgattata tcacaaaacc attcagcatc gatgagctta cacataagat
     5101 agaagtattt ttgaaacgca gcaaagtgtt tgctgaaagt ccggaagctg aatcgaaaaa
     5161 tatttatcac atcggcactt atactttcga tttcaaggaa ttgaaactct ttaataccac
     5221 acagcaacat acactcacca ttcgcgaagc tgaactatta cgcatgcttt ctattcaacg
     5281 aaatcaggta gttcgccgcg aggcaatttt gcatcaggta tggggtgaag acaattatta
     5341 tatcggcagg tcgcttgatg tatttatttc acgcctcagg aaattatttg cagatgaccc
     5401 tggagtggaa atcgaaaacg taaggggagt agggttcaaa tttaatgtag ccgactaagt
     5461 tgatttaaag gatcaatcat taaggcactg attatttttt ttcctcctca ttaatgcata
     5521 aaaaaaggag tctctttcga aactcctttt taataaaatc atatcaattt acaccagacc
     5581 ttgtgccagc attgaatcgg ctacttttac gaagcctccg atatttgcgc ctttcacata
     5641 gttgatgtat tttccttctt taccgtaagt aacgcaggtc ttgtggatgt tgtgcatgat
     5701 gtttttgagg tgattatcta cttcctctct tgtccagttc atgcgcaaac tgttttgact
     5761 catttcaaga ccactggtag ctacaccacc ggcattactt gctttacccg gagcatataa
     5821 gattttattt tccaggaaag cggtaattgc ttcaggagta caaggcatat tcgcaccctc
     5881 tgcaacacat accacaccat ttttaatcag cacttttgca tccttgccgt tgagctcatt
     5941 ttgcgtagcg caaggcaggt cgatatcaca ttttacttcc caaggtgttt taccggcaat
     6001 gtattggcat ttcactttgt cggcgtaatc tttaatgcgt ccgcgcatgt tattcttgat
     6061 atccatcagc atagcaagtt tttctgttgt gaagccattt ttatcgtaga tgtatccgtc
     6121 actgtcgctg gcggtaacaa cttttgctcc gagttcgaga catttttcga tggcatattg
     6181 tgctacgttt ccgcttcctg atacggttac tgttttgcct ttgaatgatg ctttgcgtgt
     6241 gcccatcatt tcctgagcga aataaacgca accatatcct gttgcttcag ggcgaattaa
     6301 actgccgccc cattccaatc ctttaccggt aagtactccc gtaaattcat tgcgcaactt
     6361 tttatacatc ccaaagaggt atccgatttc tcgtccgccc acaccgatat cacctgcagg
     6421 aacgtcggta tttgcaccga tatgacggtt caactccatc ataaagcttt ggcagaagcg
     6481 cattacttca gcatcgctct ttcctttagg atcaaaattc gatccacctt ttccaccacc
     6541 catcggtagt gtcgtaaggc tattcttgaa tacctgctca aaagcgagga atttcaggat
     6601 cgacaggtta acagaagggt gaaagcgcaa tccgcccttg tacggtccga tggcactgtt
     6661 catttgaatg cggtaaccgc gatttacttg cacattgccc tagtcatcaa cccacggtac
     6721 gcgaaacatc atcacgcgct caggttcggc tatgcgttcc agaatttttg cttccttgta
     6781 cttggggttg tcctctataa atgggataac ggcctcggca acttcgaaaa ctgcttgtaa
     6841 gaactctttt tctcccgggt ttctggattc taccagggtc atgaatgctt taacgttctt
     6901 ttccataggt taagtggttt tttgtggtgt gaagctagga gaaatcctga tagttccaaa
     6961 tggattaaat cacctttttt agtgacctgt cacctcaaaa gttccgaaac ggggggtaaa
     7021 tggccctcca cacgccagtt tccttcctca tgagagatat gacaccacac atgactcacg
     7081 ccattggtaa cccacaacca gggcacctgt agtgtcagat tatatcgcgc agcctggtca
     7141 actgctgcct gtgaaagttt aatgtctgat gctttgcact ctacaatcaa aacaggtttt
     7201 ccggcgtgat tatacaatac aatatcggtg cgacgagtga gagtatttac cgttagttgc
     7261 ttttcgacgc tgatgagtga ttttggaaag ccgtgatcgg tgatgagata atgtatgata
     7321 tgttgcctaa cccattcttc aggagtaagg acaacatatt tttttcgaat gatatcatag
     7381 atgcgcctgc cgctttctga ttcctgcaca cgcagatcat atgtggagaa ttgcaatgac
     7441 accctgtaaa attaagttat ttttgtgcca tggactatcg cgatctgatc aatgatataa
     7501 aagcccgcaa attcaagccc gtgtatttat tgcatggcga tgaggcttat tatatcgata
     7561 agatcgctga atatatggag gataatattc ttcctcctga tcaaaaggat ttcaatttca
     7621 gtgtttttta tggaaaggat acgcatcctc aaacagtgat agacaccagc atgcgctttc
     7681 caatgttttc tgactttaat gtcgttatgc tgaaagaagc acaaatgatg aaaggaaagg
     7741 gaaacgaaat tgataagctg gaaacttata ttgaaaaggc atcaccaaca accattttaa
     7801 tcatctgtta taaagagggc cgatttgatg cgcggaaacg catgttcaaa ctcattaaag
     7861 agaaaggtgt catatttgag tctgaaacga tcaaagaatc tgagcttccg atgttcattg
     7921 agtcatacct caaacggaaa aaagcttcca ttgattcagc tgctttgcgg ttgttagtgc
     7981 aatatcttgg tgcagatctt gccaaaatgt cgaatgagct cgataagctt tgtatcaatc
     8041 ttccggaaag cggaaggatt actgtcgatc agattgaaaa aaatataggt attagcaagg
     8101 attataatgt atatgaattg caaagtgcgt tgcttacaaa aaacttcgct ctggtttata
     8161 caatagtatc ttatatcaat gacaatcaaa aggctaatcc ttttattttc acgatatcta
     8221 atattcatgc ggcctttcag cgcctatatc attatttgct tggtggtgaa gtgagtgatt
     8281 ttgatatgta caaggtttat caaatacaca gttcgcagaa gaatgaatat aaagctgcgc
     8341 gcagattgta tacggtgcag cgcgtggaag agatttttga attattattg gattacgacc
     8401 tccgctcaaa aggactaaac aacggcgaaa ccgagcacga cgaattattg cgtgaaatgg
     8461 tttacaaaat catgtcgtga aaattcctga attaattaaa gagatgcaag ataggtgagt
     8521 gcctgttgtt tatctgcatg catggcttca accaaggatg tggcggaatc aaatttttta
     8581 tccttccgca aaaaatgcaa aaattgaatg gtaatcgttt ccccataaat ggattcattg
     8641 aagtcgagaa tatgtacttc aacgcgcgat tctgttttat tgaatgttgg tcgggtgcca
     8701 atgcttaata atccaaagta ctctccggga tgttgtttca tcactacacg caccacataa
     8761 acgccatttg ccggaatgag tttatagctg tcttcaacaa atatattagc ggttgggaat
     8821 ccgagtgttc tgccgatctg atctcccttt accaccggac cggtcagggc ataattataa
     8881 cccaataatt cattggcgga atcaatattg ccttcgagta aaaatgcacg aatttttgaa
     8941 gagcttaccg ttaaagaatc gatgatatgt gccggtatct cttcaacgtg tatggcataa
     9001 cctccggcaa tagattttaa taaatggata tcgcctgtcc tgtttttgcc gaatttatga
     9061 tcatatccga aaacgattac ggcaggatgg aatttttcaa tcaaaaaagt acgcacataa
     9121 tcttctgcgc tgatgtcaga aaattctctc gaaaaaggaa ccacaaccac aacatcaact
     9181 cctcgcgctt gtaacttact taatttttcc tccagcgtat tgagatgata caccggtgat
     9241 cccggagtaa taatttgacg tggatgcgga taaaaagtaa tgataacact tttgccattc
     9301 ctttttttag cttcttcggt aatgcggtct ataattgcgc aatgcccgtg atgcacgcca
     9361 tcaaaggtgc ctatggtaac cacacatcct gttagatcgg gcagcgattg taagtttctg
     9421 atcacaagca tggcagcaaa ggtaattatt gtatgttatt gcgactgatg aagatgttga
     9481 tttgattgtc gaaggaaatc aataaccgta tctaaagtat aggcattttc gagatgataa
     9541 ttcccgattt ttgttctgca aagggctgat aaatatccgc cacatcctaa aaattgtcca
     9601 acatcatgcg ccaaagaccg aatgtaagtc cctttgctgc aatgcacccg aaagtcgata
     9661 tgcggattgc gaaatgcgat aatttcaaat tggaatattt caatcggacg cgggcgcatt
     9721 ttcatttctt cgccttcacg ggcataagta taggcaggtt taccttgaat tttaatggaa
     9781 gaatgcgcag gggggagttg catgaaagct ccggtgaaat gttgagcagc ctgttgcacc
     9841 aataaatcag taatatgttc agtaacggtt attgattcaa caggagtctc cagatcataa
     9901 cttgctgtaa cacctcctaa ggtgatgatg ccggtatatt ccttatccag attttgcgtt
     9961 tcatcggcgc gcttagttgc cttgcctgtc agcacaataa gtaatccgga tgcaagtgga
    10021 tcaagggtgc ctgcgtgacc aacttttagt tttttattac cggtaaatgt tttcaaaggg
    10081 ccgcgcagtc ttgcaataac atcgtgactc gtccattgca agggtttatt caataaaaga
    10141 aattttcctt cggctatttc ttccggtgtg aacaacatga tcacaaaatt tgcatatcaa
    10201 cgcctgcggc aattaaaatg aggataattg cgcctacaat tattcgataa taaccgaaaa
    10261 ttttgaatcc atgtttagta agatatccca caaaactcct catggcaagt attgctataa
    10321 taaatgcgat aacatttcca ataataaatg cagtcatatt atcgtggttg ttgagtagca
    10381 attcatatgg atgcaatcct ttatttgcac cg
//
LOCUS       DAOQOU010000116        10400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3358_length_10400_cov_5.912905, whole genome shotgun sequence.
ACCESSION   DAOQOU010000116 DAOQOU010000000
VERSION     DAOQOU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3358_length_10400_cov_5.912905"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLM90_10225"
     CDS             complement(<1..557)
                     /locus_tag="PLM90_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ89794.1"
                     /translation="MPAKRKEKIIETLLAFSGGITILTTIGILTVLVIESIAFFKEVS
                     IIDFLTDTQWTPLFADKHFGIMALVSGTLLTSMIAIMVALPIGLSIAIYLNEYANPKI
                     RQTVKPVLEILAAIPTVVYGFFALTVVTPFLQTIIPGLAGFNSISPGIVMGIMIIPFI
                     SSLSEDALYAVPKSLREASYGMGAT"
     gene            complement(583..1533)
                     /locus_tag="PLM90_10230"
     CDS             complement(583..1533)
                     /locus_tag="PLM90_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HMZ89795.1"
                     /translation="MLKNLSYLGVIAVIGLSSCGGNKGGEAAEALTGSVMIDGSSTVY
                     PISEAVAEEFRAEQPDVNVALGESGTGGGMKKFGRGEIDICDASRPIKDSEKAACDSA
                     GIKYLELEIAYDGIAVMVNPKNTWVDKMTVAELKMIWESAAQGKIKTWNQVRPEWPNS
                     EIHLYSPGTASGTYEYFTEVICGKKGDSRGDINANEDDNTLVNGIAGDEGALGYFGMA
                     YYENNTDKLKIVPIDNGSGGVIPTTETVLSKTYAPLSRPLYIYVNEASMAKPQVLAFV
                     KYYMDNVEALSKEVGYVPLQSDIYTAMKQKLAAYNKPAAE"
     gene            1721..2368
                     /locus_tag="PLM90_10235"
     CDS             1721..2368
                     /locus_tag="PLM90_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMZ89796.1"
                     /translation="MSIDRVLRFFQPKDKTFYPLFDKAADNIVVIAELLNKVVQEKDQ
                     QRREELIREIEQVEHQGDEITHEVLNNLNIVFITPFDREDIHALITSIDDVADHVKDA
                     ALRIRLFKPQALPASIVKLSDLICHGTTEVRKAIKELNDMRNLGKLKKLIIDINNVEN
                     QADAVFELAMTDLFANEKDPIEIIKIKDIIAKMESATDRCEDIANVIESIIVKTT"
     gene            2372..3391
                     /locus_tag="PLM90_10240"
     CDS             2372..3391
                     /locus_tag="PLM90_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMZ89797.1"
                     /translation="MWGIEPIIIVTVVLALVFDFINGANDAANSISTIVATRVLSPLK
                     AVIWAAFFNFAALWVFHSTEVSKTMSKGVIDAGVVDGDNYFILCSLLGAILWGWLCTR
                     FGMPISLSHTLIGGLIGPALVKSGPDALLWWEPDHKGLGWIATFIILAPIIGLILAYI
                     LQIITFWIFRAFRPNRVNSFFQIMQLASSAAFSLGHGSNDAQKTAGIIILLLASNGHV
                     ALDAPPQWIFYMCYIVIGLGTLIGGWRVVKTMGHNLTPLKPMGGFCAETAGAITLFST
                     ASLGIPASTTHTITGAIMGVGTARRVSAVRWGVAAKIIWAWVLTIPATIIMSGIIYWI
                     VSQMV"
     gene            complement(3388..4197)
                     /locus_tag="PLM90_10245"
     CDS             complement(3388..4197)
                     /locus_tag="PLM90_10245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMZ89798.1"
                     /translation="MSDSAENVSLPLEDISHKVMALVKPIGAYMREQRKSFDGVAISK
                     KGIRDFVTEVDMQSERQLVAGLRNIFPDAGFVTEENTVEQIDTYYKWIIDPLDGTMNY
                     VHGIPMYSISIALQAGNKFVLGVVYEVANDEMFYTWQGAASYCNGKIISVSACHTLQD
                     ALIATGFPYIRNDRRTREISNTLKFFLDNGRDIRRLGTAATDLCYVACGRQDVYYEGF
                     LNIWDIAAGILILQNAGGVAVNFKGEENYNEGRIVGTNPNLIGDVLRGISF"
     gene            complement(4190..5854)
                     /gene="lnt"
                     /locus_tag="PLM90_10250"
     CDS             complement(4190..5854)
                     /gene="lnt"
                     /locus_tag="PLM90_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HMZ89799.1"
                     /translation="MQGSVNRTLGLFLGLPLLSGVLFWWSWPPMHTAIPIFFAFVPLL
                     FAERLISDSYTKYTGLKVWFALFLGLLVWNLATTWWVQKASVAGGLFANIANPILMTI
                     PLMLMRKVRLKFGKLAGYLAFVVFWMSFEKIHLTWELTWPWLTVGNVFAAQYSWVQWY
                     EITGVFGGTLWVLLINIWVFHAIAEYKQPLISATKKKQLIFHALGILIIIALPILISL
                     QMYRNYQDKGKPVQFTVLQPNYDPYTEKFTIPYRVQMEEMLRTSLQHVNSETDFLIWP
                     ETAIQTDVWLDKLSWQKPVRDVSKAIDSFPNLVCLIGINGYEKYPSADKATATARHLE
                     YASPVTGKPDTIYFDVYNTALALNSEGPIGYYHKSKLVPGVERMPYPEYMKLLNYFTI
                     NLGGIEGSLGIQKDRTVFFNKDSVGIAPVICYESIFGEYVGDYINKGANIICIITNDG
                     WWGDTDGYKQHCMYASLRAIESRRAIARSANTGISCFIDQRGDISQAQPWNVQSAISA
                     SLPANSAFTFYTRHGDYLARFALWLSGGMLVILLIQRLLNKFSKRV"
     gene            5985..6968
                     /locus_tag="PLM90_10255"
     CDS             5985..6968
                     /locus_tag="PLM90_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ89800.1"
                     /translation="MVKIGVLGAGHLGKIHIKLLKEIPTVELIGFYDPSDANAEQVVN
                     QYGVPRFTNMHRLIDACDAIDIVTPTISHYECARAAIKKAKHIFIEKPLANTVSEAKS
                     LVALSHEAHIKAQVGHVERFNPAFLSVKDQHFAPMFIESHRLSTFNPRGTDVSVVLDL
                     MIHDIDIVLHLVKSNVKKVSASGVKVISNQPDIANARLEFDNGCVANLTASRISMKKM
                     RKMRLFQRDSYVSIDFADNVTEILTLSDAKDHPDALTIDINTGDQFSKKAVRLDRPEV
                     QPVNAIKLELEMFANAIIHDTETPVTIEDGFRALQVAHMILQKVEKNAAFH"
     gene            6983..7855
                     /locus_tag="PLM90_10260"
     CDS             6983..7855
                     /locus_tag="PLM90_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89801.1"
                     /translation="MLNKTREILIILFAVGLFAGCEIINPEEITPSYVYINDFTLTTN
                     LETQGYASSKITDGWIYINGTFIGAYELPATVPVLTEGETDIIIYPGIKENGISGVSM
                     IYPFYNAYTITRTLTAGSTDTLHPSCTYKPSAGIQFDMLERFESSNAFGGVETEVGLN
                     TTTDPSLVFEGNRSAIANFYPDLDTFRVASSEPIAFPGADKQMFLELDYLAGMDFNVW
                     LKCNTTDQPVYDEVLTITEKDYWNKIYVNLNPSLQFFAQYSPISIQLEFRAVNYDNDT
                     SSIMFDNVKIIQTK"
     gene            7855..9279
                     /locus_tag="PLM90_10265"
     CDS             7855..9279
                     /locus_tag="PLM90_10265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HMZ89802.1"
                     /translation="MRFKKKIGILTYIIFDYMSAMVVWAGLFFYRKFVIEDLPFDIDG
                     QVLSDNNFFAGIISIPVIWLGLYFIAGTYTDIYRKSRLAEITRTFVVTFIGVLIIFFA
                     LLLDDYISDYRDYYQIFFVLLFGQFLLTTIVRMIILTRAKRQLQKGVVAYNTIIIGGD
                     QRAYSIYREITHYKKSLGYAFVGYLEANGDHHSDLEGHMPCLGNLSQLESILDQRNVD
                     EVIIAIETSEHHLLNNLIDTVAGKGNIVIKIVPDMYDILSGSVKMSNVLGAVLIEIYP
                     DLMVGWQRMIKRALDVTVASIGFIVLWPLYLFIAIRVKMSSKGPIFYHQERIGLHNKP
                     FTIYKFRSMYIDAEQNGPALSSENDERITPWGRIMRKWRFDELPQIFNILKGEMSLVG
                     PRPERRHYIDQIVKIAPSYKHLTKVKPGLTSWGMVKFGYASNVDEMVQRMKYDLLYIE
                     NMSLAIDFKILFYTLLIIFQGKGK"
     gene            9349..>10400
                     /locus_tag="PLM90_10270"
     CDS             9349..>10400
                     /locus_tag="PLM90_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HMZ89803.1"
                     /translation="MSYQTPDGLSSEEVLASRKLHGSNVQEVQQRNLLVGIILEIVAE
                     PLFILLSVTMVLYFILGEIQEGLILMVALGIVSGISIFQEQRSRNAVNALKDLTSPRT
                     QVIRNKEQISIHTDEIVVGDIILLEDGNIIPADATILFSRDLTVNESVLTGESLPLYK
                     SADSADNLLFQGTTIVGGSATATVTAIGLNTRLGEIGAKLSDIAVQKTPLQLQIRKFI
                     QSMVIVGVIAFLIVCIVNYLTSKNIIHSLLHGLTLAMSILPEEIPVAFSTFMALGAFH
                     LYKKNVIAKSPYTVETLGAATVICSDKTGTITENLMKPIAIYDAIDDNVHDFNISRPA
                     YHPLIEFAMWASEPEP"
BASE COUNT         3092 a         2123 c         2136 g         3049 t
ORIGIN      
        1 cttgttgcgc ccataccata acttgcttcg cgaagtgact tcggcacagc atataaagca
       61 tcttcgctta aggaggaaat aaatgggatg atcataatac ccattacgat acccggcgag
      121 atagaattaa atcctgccaa tccgggaata atcgtctgca aaaatggcgt caccaccgtt
      181 agtgcgaaaa acccgtatac tacggtagga attgctgcca aaatttcaag aacaggtttt
      241 actgtttgtc gaatttttgg attagcatat tcattcaggt aaatggcaat actcaaacca
      301 atgggtaatg ccaccatgat agcgatcatg gaggtaagca atgttcccga taccaaagcc
      361 atgataccaa aatgcttatc ggcaaatagc ggggtccatt gtgtatctgt aagaaagtcg
      421 atgatggata cttctttaaa gaaggcaatc gactcgatca ccaataccgt taaaatacca
      481 atagtggtga gaattgttat cccaccacta aaggccaaaa gagtttctat tattttttcc
      541 ttacgctttg caggcatagg gttttactat taattgaaaa aattattctg ctgcaggctt
      601 attatacgcc gccaattttt gtttcatggc agtgtaaata tcagattgca gcggcacata
      661 tcctacttct ttgcttaatg cttcaacatt atccatataa tatttcacga aagccaatac
      721 ttgcggctta gccattgatg cttcgtttac atatatatac aacggacgag aaagcggtgc
      781 ataggtcttg ctaagaacag tttcggtagt tggaattaca ccgcctgatc cattgtcgat
      841 tggcacaatt ttcagtttgt cggtattatt ttcataatat gccattccga aatacccgag
      901 ggcaccttca tcacctgcta taccatttac aagggtattg tcatcttcgt tggcattaat
      961 atcaccacga gaatcgcctt tcttaccgca aattacttcg gtaaaatatt cataggttcc
     1021 ggaagcagtt cccgggctat aaagatggat ttcagaattt ggccattcag gacgcacttg
     1081 attccaggtt ttgattttgc cttgagcagc agattcccaa atcattttga gttcggcaac
     1141 agtcattttg tctacccagg tattcttagg atttaccatc acggcaatac cgtcataagc
     1201 aatttccaac tcaagatatt tgattccggc agagtcgcaa gcagcttttt cgctgtcttt
     1261 gatagggcgg ctggcgtcgc agatatcgat ttcgccgcga ccgaatttct tcattccacc
     1321 acctgttccg ctttcaccaa gggcaacgtt cacgtccggt tgctcggcac gaaactcttc
     1381 ggcaacggct tcagaaatgg ggtatacggt gcttgatcca tcaatcataa cagatccggt
     1441 aagggcttct gcagcctctc cacctttgtt tccaccgcag gaactcagtc cgattacggc
     1501 tataacacct aaatagctta agtttttcaa cataattgca gttttttatc ggggacaaag
     1561 ctatccgggg tgtattaact caatgttaag taagcattaa gataatccta tttatacaat
     1621 ttcttaatca tgggaccggt ttaggtcaat gttaagtgtt tgtgattgtt aattttagtt
     1681 taattttaca gacacttttt aacgaactaa gcccattacc atgtctattg atcgcgttct
     1741 tcgattcttt caaccgaaag acaaaacctt ttatcccctg tttgacaaag cagccgacaa
     1801 cattgttgtg attgccgaat tactgaacaa agtagttcag gaaaaagacc agcagcgccg
     1861 cgaagagtta attcgcgaga ttgagcaggt tgaacatcag ggagatgaga ttacacatga
     1921 ggttttgaat aacttgaata ttgtgttcat cacaccgttt gatcgcgaag acattcatgc
     1981 gctcattaca tcaattgacg atgtagccga tcacgtaaaa gatgctgcac tgcgcattcg
     2041 tcttttcaaa ccgcaagcat tacctgcaag tattgtaaag ctatccgact taatctgcca
     2101 tggaacaact gaagtgcgca aggcgattaa agaattgaac gatatgcgca atcttggcaa
     2161 attaaagaaa ctgattattg atatcaacaa cgttgaaaat caggctgacg cagttttcga
     2221 attagcgatg accgaccttt tcgcaaatga aaaggatcct atcgaaatca ttaaaatcaa
     2281 ggatatcatt gctaaaatgg aaagtgcaac cgatcgttgt gaagatatag ccaatgtgat
     2341 cgagtccatc atcgttaaaa caacttaatg tatgtgggga atcgaaccaa tcattattgt
     2401 cacggttgtt cttgctctcg tgtttgattt tatcaacgga gcaaacgatg ccgccaattc
     2461 gatatcaact attgtagcga caagagttct aagcccatta aaagcggtga tatgggctgc
     2521 atttttcaac tttgctgcac tatgggtatt tcacagcaca gaagtatcca aaacgatgag
     2581 taaaggtgtt atcgatgccg gagttgtgga tggcgataac tattttatcc tctgttcatt
     2641 gttaggcgca attttgtggg gatggctatg tactcggttt ggcatgccca tcagcttgtc
     2701 acatacatta ataggcggac tcataggccc ggcccttgta aaaagtggtc ccgatgcgtt
     2761 gttatggtgg gaaccagatc acaaaggatt aggatggata gctacgttta tcattttagc
     2821 tcccatcatc ggattaattt tagcatatat acttcaaata ataacctttt ggatattcag
     2881 agcctttagg cctaacaggg tgaatagctt ttttcagatc atgcagttgg catcatcagc
     2941 agcttttagc ctcggacatg gaagcaacga tgctcaaaaa actgcgggaa ttattatttt
     3001 attgctcgct tcaaacggtc atgttgcact agatgccccc cctcaatgga ttttctatat
     3061 gtgctatatc gtaattggtc tgggaacatt gattggtgga tggcgagtgg taaagacgat
     3121 gggacacaac cttacgccat taaaaccaat gggcggattt tgcgcagaaa ccgcgggggc
     3181 aatcacattg tttagcactg cttcattagg tattcctgct tcaacaacac ataccattac
     3241 cggcgccatt atgggtgttg gaacagctag gcgagtaagt gccgtaagat ggggcgttgc
     3301 cgcaaaaatt atttgggcat gggtactgac tattcctgct accattatta tgagcggaat
     3361 aatttattgg atagtatcgc aaatggtcta aaaactaata ccgcgcaaca catcacctat
     3421 caggttagga tttgttccta ctatacgacc ctcattgtaa ttttcttccc ctttaaaatt
     3481 aacagctacg ccgccggcat tttgtaaaat cagaatgcct gcggcgatat cccatatatt
     3541 caggaaccct tcataatata catcctgcct gccacaagcc acataacaca aatcagtagc
     3601 tgctgttccc agtctgcgta tatcccgacc attgtctaaa aaaaatttta gagtattcga
     3661 aatttctcgc gttcgccggt catttctgat ataaggaaat ccggtagcaa ttaaggcatc
     3721 ttgcaaagta tgacatgcag aaacagaaat gattttacca ttgcaatatg aagcagctcc
     3781 ttgccaggta taaaacattt catcattcgc tacttcatat acaacgccga gtacgaattt
     3841 atttcctgct tgcaatgcaa tgctgatact atacattgga atgccatgca cataattcat
     3901 cgtgccatcc aatggatcga ttatccactt ataatatgta tcaatttgtt cgacagtatt
     3961 ctcttcagta acgaatcctg catcggggaa aatattgcgc aatcctgcaa caagttgacg
     4021 ttcggattgc atatctactt ccgtaacaaa atcgcgaata cctttttttg aaatagcaac
     4081 gccatcgaat gattttcgtt gctcacgcat ataagcaccg atgggtttca caagagccat
     4141 cactttgtgg gatatatctt ctaatggaag actgacattt tctgcagaat cagacacgtt
     4201 tcgaaaattt attcaacaat ctctgaatta ataatattac caacatacct ccgctcaacc
     4261 ataaagcaaa tcgggcaagg taatcgccat gacgcgtata aaatgtaaat gcactatttg
     4321 cgggcagaga ggctgatatt gcgctttgaa cattccaggg ttgagcctgc gaaatatcgc
     4381 cacgctgatc aataaaacaa ctgatgcccg tgttcgcaga tcgggcaatg gctctgcgcg
     4441 attctattgc tctgagactg gcatacatac aatgttgttt gtatccatct gtatcgcccc
     4501 accatccgtc attcgtgatg atacaaataa tattggcccc tttattaata taatcgccaa
     4561 catactcacc aaaaatgctc tcataacaaa tcaccggtgc tatgccgaca gaatctttat
     4621 taaaaaagac cgttctgtcc ttttgaattc caagtgaacc ttcaattcct ccgaggttaa
     4681 tagtaaaata attcagcaat ttcatgtatt ccggatatgg catacgttca acaccgggta
     4741 ctaatttcga tttatgataa taccctatcg gcccttcact atttaaagca agtgctgtat
     4801 tatacacatc aaaataaatc gtatccggtt ttcctgtaac aggactggca tattccagat
     4861 gtcgcgcagt ggctgttgct ttatcggcag atggatattt ttcgtatcca tttattccta
     4921 tcaggcaaac aagattcgga aaactatcaa ttgctttgct cacatctcta accggttttt
     4981 gccacgacaa tttatctaac caaacatcgg tttgaatagc tgtttcaggc caaattaaaa
     5041 agtcggtttc actattgaca tgttgcaagg aagtgcgcaa catttcttcc atttgcacac
     5101 gatatgggat ggtaaatttc tcagtgtatg gatcgtaatt gggttgcaat accgtaaatt
     5161 gaacaggctt acctttatcc tgatagttgc gatacatctg caaacttatc agaataggca
     5221 aggcaataat gatgagtatg cctaaagcgt gaaaaatcag ttgtttcttt ttcgttgcag
     5281 agataagtgg ttgcttgtat tcagctatcg catgaaatac ccaaatattg attaacagca
     5341 cccatagtgt tccgccgaaa acaccggtaa tttcatacca ttgcacccat gaatattgcg
     5401 ctgcgaatac atttcccacc gtcagccaag gccatgtaag ttcccaggtg agatgtatct
     5461 tttcgaagct catccaaaac acgacaaaag ccagatatcc cgccagtttg ccgaatttca
     5521 atctcacttt tcgcatcagc atcagcggaa tggtcatcag aatgggattg gcaatattgg
     5581 caaacaatcc accggctaca gaggcctttt gcacccacca agtggtagca agattccata
     5641 ctaacaagcc taaaaagagt gcgaaccaaa ccttcaggcc tgtatacttc gtgtagctat
     5701 ctgaaatgag cctttcggca aaaaggaggg gcacaaaggc gaaaaatata ggtattgccg
     5761 tatgcattgg cggccaggac caccaaaaaa ggactcctga aaggagagga agtcccaaaa
     5821 acagccccaa tgtccgattt actgatcctt gcatacctgt ggatcaaagg taaacgattt
     5881 cgacgggtat aatcatgtgg tcaattgtgt tggtgatagg tggacatttc atttgctgaa
     5941 aattgagcac tctgtgcgaa aggcataact ttgcaagcta agatatggtt aagatcgggg
     6001 tattaggtgc aggacatctg gggaagatcc acatcaaact gctaaaagaa attccaactg
     6061 tggaactgat cggtttttac gacccttcgg atgccaatgc tgagcaggtt gtaaatcaat
     6121 atggtgtgcc acggtttaca aatatgcatc gactcattga tgcctgcgat gccattgata
     6181 ttgtaacacc caccatttca cattacgagt gtgccagggc cgccattaaa aaggccaaac
     6241 acatcttcat agagaaaccg cttgccaata cagtaagcga ggccaagagc ttggttgcgc
     6301 tatcgcatga ggcacatata aaggcacaag ttggtcatgt tgagcgattt aatccggctt
     6361 ttttatcggt gaaagatcaa cattttgctc ctatgttcat cgagtcgcat cggctcagta
     6421 cattcaaccc ccgtggaacc gatgtatctg ttgtgctgga cctgatgatt cacgatattg
     6481 acattgtatt gcatctggta aagtctaatg ttaaaaaggt atctgcaagt ggtgttaagg
     6541 tgatatcaaa ccaacccgac attgcaaatg cacgtcttga atttgataat ggttgcgtag
     6601 caaatcttac cgccagcagg atctcgatga aaaaaatgcg aaaaatgcgc ttatttcaac
     6661 gcgattcgta tgtgtcgata gattttgccg ataatgtgac agaaatatta acgctttcag
     6721 atgcgaaaga tcatcctgat gcgttaacca ttgatattaa tacgggagat cagttcagca
     6781 aaaaagctgt aagactggac agacctgaag tgcaacctgt caatgcaata aaactggaat
     6841 tggaaatgtt tgccaatgcc atcatccatg atactgaaac acctgttacg atagaagatg
     6901 gttttcgcgc attacaggta gcgcacatga ttttacaaaa agttgaaaaa aatgctgcgt
     6961 tccactaaat aaggagatat caatgttaaa taaaacacgg gaaatattaa tcattctttt
     7021 tgctgttgga ttatttgcgg gatgtgaaat aatcaatcct gaagaaatta caccatcata
     7081 tgtctatata aatgatttca cattaacaac taatctcgaa acgcagggat atgccagcag
     7141 taaaataact gacggctgga tatacatcaa tggaactttt attggtgctt atgaacttcc
     7201 ggcaaccgtt cccgtgctaa cggaaggtga aactgacatt attatatatc cgggcattaa
     7261 agaaaatggt atcagcgggg tctcgatgat atatccattt tataacgcat ataccattac
     7321 aagaacgctt actgccggat caaccgacac cttgcatccg tcttgcacat acaagccatc
     7381 tgccggaatt caattcgata tgcttgaacg ttttgaaagc agcaacgctt ttggaggtgt
     7441 cgaaactgag gttggactga atactaccac tgatccatct ttggtgtttg aaggtaacag
     7501 gtcagcaatt gcgaactttt acccggatct ggatactttc cgtgtagcat caagcgaacc
     7561 gattgcattt cccggtgccg ataagcaaat gtttcttgaa ttggattatt tagcaggcat
     7621 ggattttaat gtatggttga aatgtaatac aactgatcag cctgtgtatg atgaggtttt
     7681 aaccatcact gaaaaagatt actggaataa gatctatgta aacctcaatc cttcccttca
     7741 gttctttgca caatattctc ccatcagtat tcagttagaa ttcagagctg tgaattatga
     7801 taatgacacg agttccatta tgttcgataa tgtgaaaatc attcaaacaa aataatgcgt
     7861 ttcaaaaaga agatcgggat acttacctat atcattttcg actatatgtc ggcaatggtg
     7921 gtatgggccg gtttattctt ttatcgcaaa tttgttattg aagatctgcc atttgacatc
     7981 gacggacagg tgttgagcga caataatttc tttgccggga taattagcat accggttatc
     8041 tggcttggct tgtatttcat tgcaggcacc tataccgaca tttacagaaa atcgcgactg
     8101 gccgagatca cacgcacgtt tgtggtaaca tttattggcg tgttgattat attctttgcc
     8161 ttgctgctgg atgattatat cagcgactat cgcgattatt atcaaatttt ctttgttcta
     8221 ttatttggtc agtttttgct tacaacaatt gtccgcatga tcattctgac cagggcaaag
     8281 cggcaattgc aaaaaggtgt agtggcttac aacactatta ttattggtgg agaccagcgt
     8341 gcttattcaa tttacagaga gatcacacat tataagaaat cactgggata tgcatttgtc
     8401 ggatacctgg aagctaatgg tgatcatcat agtgatttgg aaggacatat gccttgcctg
     8461 ggaaatttga gccagctgga atccatttta gaccagcgca atgtagatga agtaattatt
     8521 gcgatagaga catcggaaca tcatctgctc aataatctta ttgataccgt agcaggaaaa
     8581 ggcaatatcg tgataaaaat tgttcctgac atgtatgata tcctgtcggg cagtgtgaag
     8641 atgagtaatg tattgggcgc agtattaatc gaaatttatc ccgacctgat ggtaggttgg
     8701 caaaggatga tcaaaagagc gttggatgta actgttgcat caataggatt cattgtatta
     8761 tggcctttgt atttatttat tgccattcgt gtgaagatgt cgtcaaaagg tccgattttc
     8821 tatcatcagg aacgtattgg tttgcataat aagcctttta ccatttataa gtttcgctct
     8881 atgtatatcg atgcagagca aaacggaccg gcattatcca gcgagaatga cgaacgtatc
     8941 accccctggg gccgtataat gcgcaaatgg agatttgatg agctgccaca aatatttaat
     9001 atcctgaaag gtgaaatgag ccttgtggga ccccgtcctg aacgccgaca ttatatcgat
     9061 cagattgtaa agattgctcc ctcgtataaa catcttacca aagtaaagcc gggactaaca
     9121 agctggggca tggtgaaatt cggatacgcc agtaacgtgg atgaaatggt acagcgaatg
     9181 aaatacgacc tgctgtacat agaaaacatg agcctggcaa ttgactttaa aatattattc
     9241 tatacgctgc tcatcatttt ccagggaaag ggaaaataag gaatttttgt gatatgtaat
     9301 acgaggaatg tccatgtgcg ttatggcacc ttatattacc tttgattcat gagttatcaa
     9361 actcctgacg gtttatcatc tgaagaggtg cttgcttccc ggaaattaca tggttccaat
     9421 gtgcaggaag tacagcagcg aaatttgctc gttggcatta ttctcgaaat agttgccgag
     9481 ccgcttttta ttttattgtc ggtaaccatg gtgttgtatt tcatacttgg cgaaatacaa
     9541 gaaggtctca tattaatggt ggccttagga attgtttccg gcatttccat atttcaagaa
     9601 caacggagca gaaatgctgt aaacgctctg aaggatctca ctagtccgcg aacacaagtc
     9661 atcagaaaca aagaacaaat ttccatacat acagatgaaa tagtggtagg tgatatcatc
     9721 ttactcgagg atggtaatat tattccggcg gatgctacaa ttctattttc aagagattta
     9781 actgttaatg aaagtgtgtt gacaggagag tcgcttcctt tatataaatc ggccgacagc
     9841 gctgataatt tactttttca aggaacaacc atagtaggcg gctcagcaac tgcaacggtt
     9901 accgcaattg gattaaacac acgtttgggt gagattggtg cgaaattatc cgatatcgct
     9961 gttcaaaaaa cacctttaca acttcaaatc agaaaattta tacaatcgat ggtgattgtt
    10021 ggtgtaattg catttctgat tgtatgtata gtgaattacc tgaccagtaa aaatatcatt
    10081 cacagtttat tacacggact tactcttgct atgtctattt tgcctgaaga aatccctgtt
    10141 gccttcagca catttatggc attgggtgca tttcatttgt acaagaaaaa tgtcatcgcc
    10201 aagagtccct atactgtcga gaccttagga gctgccaccg taatttgttc tgataaaaca
    10261 ggtactatta cagaaaacct gatgaagcca attgccattt acgatgcaat tgatgataat
    10321 gttcatgatt ttaatataag cagacctgca tatcatccgt taatagaatt tgctatgtgg
    10381 gcttcagagc cggagccgtt
//
LOCUS       DAOQOU010000117        10390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3364_length_10390_cov_5.819739, whole genome shotgun sequence.
ACCESSION   DAOQOU010000117 DAOQOU010000000
VERSION     DAOQOU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3364_length_10390_cov_5.819739"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1053
                     /locus_tag="PLM90_10275"
     CDS             190..1053
                     /locus_tag="PLM90_10275"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ89804.1"
                     /translation="MRLRIMKYVGVMTLLLTISMSVGAQTITGTVHDIETGETLPFVT
                     IGIPGHGLGTVAQADGKFSLRTDGAGDNDTIRISMIGYATEKTTVGAWRKKSQVDVFL
                     SPIATQLDQVTVRPRDYKKVRLGNDYHTMTVQVGFNGGGEDTVEVNPPGAELGTIMKV
                     KDGRKYFLDSCGMNLTAFTPDSAVLRINFYQLQNDEPDMLLNKEPIYVTIYKNQSEVR
                     LDLRKYDIVAEDDFVMAAEWLVDLKGKEEKILFSGGFIGAGIRYRTNSASKWKKVPVG
                     IAMFVDAVYEK"
     gene            1103..2143
                     /locus_tag="PLM90_10280"
     CDS             1103..2143
                     /locus_tag="PLM90_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HMZ89805.1"
                     /translation="MQSKQEKIASFDANGVGVANNQFFGLPFTYEESQLVLFSVPWEV
                     TVSYRAGTAKGPDAMLKASTQIDLYDNDNPNGWHNGIFTIKSSPTLKRRNTELRKIAG
                     TYIKALEQGKDMSLSGIPEKINEACATMVDQVYQQSKQILADGKIPALVGGDHSTPLG
                     LMKALAEHHGGFGILQIDAHADLRDAYEGFTYSHASIMFNALAIDNMKKLVQVGIRDL
                     CDAELQLIDNSDRRVVTYFNHEIKRAAYHGMTWGDICSRIVEELPKKVYVSFDIDGLL
                     QTFCPNTGTPVPGGLEFEQAVYLLNAIVESGRTIIGFDLVEVAPGEDEWDAIVGARML
                     YKMCNIALKGNA"
     gene            2136..2720
                     /locus_tag="PLM90_10285"
     CDS             2136..2720
                     /locus_tag="PLM90_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ89806.1"
                     /translation="MLNLRNIIVLIAICISIGCNQDHGISQIDLYDRNDKAQQISFDN
                     YDATVITFLGPECPLSENYTKTINDLDSTFKSNVHFINIFPGTFYSKEAIDSFMHTYN
                     VMQNCLLDADFDLVRELHATITPESFVFDRNGKLVYSGAIDNWAVDLGQKRQVITEFY
                     VRDVLNAMLQHKEIPYTKTTAVGCFIETEAKQHH"
     gene            2723..4117
                     /locus_tag="PLM90_10290"
     CDS             2723..4117
                     /locus_tag="PLM90_10290"
                     /inference="COORDINATES: protein motif:HMM:NF015657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89807.1"
                     /translation="MRNLILFAFLALLMTSCADKSGSNKKQADEEKVILPDVVTFNEH
                     IAPIIFENCTPCHRPHSAGPFPFTTYDEIKAKAKTISKVTQSRFMPPWPADPSYTNFK
                     GERILTVTEIAMIKKWVEDDCPVGDVAKIPAVPHYPEGSQIGTPDLVINFPDFTLEGN
                     NKDHFFLVKVPFEIPEDKYVKTIEFIPGNKKLAHHMNANLIEYQPGKKKTVFNGKKII
                     PTNLAESADVVQTEMDNKNDDGTYPVLVPLVCNYLPGVSPAMYPEGIGGFRMTKQGTF
                     FINDFHFGPTPKDAIDTSSHFNIFFDDKPPVRPTYEILLGSLGVAPVTPALVIPPNEV
                     KTFTIDYQVQETMSLLTVNPHMHLLGKSFLAYALTPQMDTIPLIRINKWDFRWQYFYT
                     FKSVVVIPGGSRIHVEAVFDNTVNNVNNPYYPPQVIIDRGDRFDSMKTTNEMLQLILT
                     YMEYQSGDEYLSLE"
     gene            4119..5315
                     /locus_tag="PLM90_10295"
     CDS             4119..5315
                     /locus_tag="PLM90_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ89808.1"
                     /translation="MRKLFPYITRPILVLSLVSLFTDMASEMLYPVMPAFLESIGFSI
                     LLIGLLEGLAEMIAGLSKGYFGKRSDIQQKRLPFVQLGYALSAISKPMMALLSYPLWI
                     FFARTTDRVGKGLRSAPRDAMLNANASKETKASVFGFHRSMDTIGAIVGPLLALIYLQ
                     CYPQDYKNLFLLAFIPGLIAVIFTWSLKEEKKAARGERVKFFSFLSYLPNAKKPYRQL
                     VIGLLAFALVNSSDVLLLLRMKQIGIDDVTIISMYIGYNIIYALLAFPIGILADKVSI
                     KYIFIAGLLIFAVVYTGFAYAESATTYYILFACYGIYAAATEGIAKAWITNITPKEEA
                     GTAIGTYTGLSSIAAFFASALAGLIWSVAGAGWAFGISAAVALLVGAYLVSIPFQRVV
                     SSANGA"
     gene            5443..6741
                     /locus_tag="PLM90_10300"
     CDS             5443..6741
                     /locus_tag="PLM90_10300"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMZ89809.1"
                     /translation="MNGNWTTTINLAIRGATIIGKFLLVYFLAKTLTVEQYGVWGIFQ
                     TSISLSLYVVGLDFYTYSSRRILDYPVEERSPMLRDQLVFYFISYLVIFPILGLLFVF
                     NEIDWRFAIFFYAILLLEHLAQETYRTFVVFSKPIIANIVLFLRTGLWPFVLLFMWFI
                     GFDELRNLKAVYLFWMAGGLSAAAVSIYFISKIKFKPVKNIPIDWKWIKQGIGVSLIF
                     FVGTVGYKIIEFADRYFVDYYHSKEDVGVYTFYAGICNIVETFVHTAVIIIFSPKLIE
                     TFHKDNYQYRKTFGTFARQVVGATLLVGVSLALLGIPLLRSLGESEYIEDYNVFLLLV
                     LSKMVLNISLIFHYILYVRKNDFPIIKATIYASVINILLNFILIPPLSIMGAAIATLV
                     SFMIILVMKVYYTRHLPEARQIIYLRFLRRKLRRKANTNH"
     gene            6753..7943
                     /locus_tag="PLM90_10305"
     CDS             6753..7943
                     /locus_tag="PLM90_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89810.1"
                     /translation="MEKAYYEHIQWWDYLIYLVYNGLLAYLLGRVGEIALNIGKQARV
                     LFFIAFEVYFFLVIGDSLFHYLPYLPDSELYSYMISSGQYPETSSENLIVIYYISIFL
                     SIICLNDPVIFTFFCIFLYALGAMLFIQAWKKADPSFNTNSETSAALLFLLWPAAALY
                     ITTPLREAFIMFGFGLFLNGFMDCIYKGKWRSVIIGSIIICAIRLQLFVFVLPVLGAL
                     VVYKSSLHKIWKSAILVGTIVAAFLVVRYVLVEHPLSPQTMAQLRNVNLDNAGPLGYG
                     RVDWDSYTDMARDYPFIIAQFMFSPFPIFVQHDPLSTFVPFLDCLFLIFLLILIFSRI
                     KTLIRQHLPVLLLCVFYIILFGGYEYHITGAVRHRMPLEALFILMAAPALSRIIFRKK
                     HAAA"
     gene            7930..8742
                     /locus_tag="PLM90_10310"
     CDS             7930..8742
                     /locus_tag="PLM90_10310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ89811.1"
                     /translation="MPQLSILLSTINDRIFQAETILNTPLPDTEFVVVHQLTEGNVAE
                     NYQSFYARFTEKPVRFIQQSAKGTGRSRNAALRAGSGNLFYLCDDDLILEPEFLDRIL
                     QASSQYPNADIFTFQIRTTEGKPYKNYPDKPYQHTLRSTATVSIVEMVIRKSAVDSEM
                     IAFDERFGLATDYNTGEEFVMLHDALTKGAVAMYIPQVLVQHPPVSSGKIYNKTVAFE
                     KGAMIARVYDWRWPFINVAFAVRKYKEYRHSAGFFAFLIAMYKGSMHFNKND"
     gene            8735..9493
                     /locus_tag="PLM90_10315"
     CDS             8735..9493
                     /locus_tag="PLM90_10315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ89812.1"
                     /translation="MTEPTVSVIMPVYNAAKYLQQAVDSVKSQTYANWELWMVNDKST
                     DNSAEIMSQLASGDARIHAIHLENNSGSAHARNIAIAQAKGKYIAFLDADDQWKPEKL
                     KLQVAFMQQHNAVLSHTAYDTISASNESLHREIHVNAKVTYRQLLKNNTIGCLTAMYN
                     AEALGKQFMPDLRKRQDYGLWLQILRNGRVAYGLDEVLATYRVGMDSLSGNKWRVLRY
                     NWMLLRKHEQLSLISSTYYFLCFLWNKTFKYLGK"
     gene            9490..10164
                     /locus_tag="PLM90_10320"
     CDS             9490..10164
                     /locus_tag="PLM90_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89813.1"
                     /translation="MKRAIIFGSSGLTGNALAKLIAEDPRYDELICFQRREVAIQYPK
                     QKNVITTFQDLHYYADLFYGTDVFCCLGTTMRNVNNDKVAYREADVDRPLAAAKAAAS
                     HGATSFTIQTALTANAKSMVFYNRIKGEVEDAVRNLGLRAVYIYRPSILTGNRQEKRT
                     GEGMGITITKFVTPILVGPWKKYRAIAAADVAKAMLIVAKSEKPGDHIFLSDRIQAIA
                     DGSTWQ"
     gene            10145..>10390
                     /locus_tag="PLM90_10325"
     CDS             10145..>10390
                     /locus_tag="PLM90_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HMZ89814.1"
                     /translation="MAARGNENYTQFVKQLSAELGFSYCGISTATELTEEAHQLEKWL
                     SKGMHGAMGYMAEHFDKRIDPRKLVPGARSVISLMYNY"
BASE COUNT         3030 a         2160 c         2174 g         3026 t
ORIGIN      
        1 gaatggaaat caaatgctgc aagcaacaat ttgcgcacag ctgccttcgt tgtagcaatc
       61 aataaaattg caagcgacta tctggcaatg ggtatcttcc catgacggag accgcataga
      121 aaagaagact cctgctcaca aagcaggagt tttttatttt cgaccatatc tgtaacattt
      181 tatgctttca tgcgccttag gattatgaag tatgttggag tgatgacact tttgctgact
      241 atcagtatgt cagtcggcgc acaaacaatt accggcacgg tccacgatat agaaaccgga
      301 gaaacattgc cttttgttac tattggtatt cccggacatg gcttgggaac agtagcacaa
      361 gccgatggga aattttctct gcgcacagat ggtgcaggcg ataatgatac cattcgcatc
      421 agtatgattg gctatgcaac ggaaaaaact actgtaggtg cctggagaaa aaaatcgcag
      481 gtagacgttt ttttgtcgcc gatagccact caacttgatc aggtaactgt gcgaccacgc
      541 gattataaaa aagtacgact tggcaatgat tatcacacca tgaccgtgca agttggattt
      601 aatggcggcg gagaggatac cgtggaagta aaccctcccg gagctgagtt aggaaccatc
      661 atgaaagtaa aagacggcag gaaatatttc ctcgattctt gcggcatgaa tctgaccgca
      721 tttacaccgg atagtgctgt tttgcgcatc aatttttatc aattgcagaa tgacgaaccg
      781 gatatgttac tcaataaaga accaatttat gtaacgatat acaaaaatca atctgaagtg
      841 cgtcttgatc ttaggaagta cgatatcgtt gcagaagatg attttgtgat ggctgctgaa
      901 tggcttgtcg accttaaagg caaggaggaa aaaatattgt tcagcggtgg atttattggc
      961 gccggaatac gctatcgcac aaacagtgca tcgaaatgga aaaaagtgcc ggtcggcatt
     1021 gccatgtttg ttgatgccgt ttacgagaaa tgatgtggcg cacaggttgc agtggtaagc
     1081 agcggcttgc tatctttgca acatgcaatc aaaacaggaa aagatagcat cctttgatgc
     1141 caatggtgtt ggtgtagcca acaatcaatt tttcggactt ccatttactt acgaagaaag
     1201 ccaattggtg ttattctcag tgccatggga agtgacagtc tcttatcgcg ccggcaccgc
     1261 caaaggacct gatgccatgc tcaaggctag cacacagatc gatctctatg acaacgataa
     1321 tccaaacggc tggcataatg ggattttcac catcaaatca tcgccaacac taaaacgtcg
     1381 caacacagaa ttgcgaaaaa ttgcaggcac ttatatcaag gctttagaac agggaaaaga
     1441 tatgtcgctt tcaggtattc ctgaaaagat aaatgaagcc tgtgcaacaa tggttgatca
     1501 ggtttatcag cagtcgaaac aaattcttgc cgacggaaaa attcctgcgc ttgtgggagg
     1561 tgatcacagc acaccactcg gactcatgaa agcactcgca gaacatcacg gcggattcgg
     1621 cattctgcaa attgatgcac acgccgatct tagagatgct tatgaaggat ttacctattc
     1681 acatgccagc atcatgttca atgcgctcgc tatcgataat atgaaaaaac tcgtgcaagt
     1741 gggtattcgc gatctgtgtg atgccgaatt acaacttatc gataattctg atcgtcgcgt
     1801 tgtaacctat tttaatcatg aaataaaacg cgcagcatat cacggaatga catggggcga
     1861 tatttgctcc cgcattgttg aagagcttcc gaaaaaagta tatgtcagct tcgatatcga
     1921 tggcttactg caaacatttt gtccgaatac aggcacacct gttcccggtg gacttgaatt
     1981 tgaacaggct gtttatttat taaatgcaat tgtcgagtct ggccggacaa tcatcggatt
     2041 tgacctcgtg gaagttgccc ccggagaaga tgaatgggat gccattgttg gtgcacgtat
     2101 gctgtataaa atgtgcaata ttgcgttgaa aggaaatgct taatctgcga aatatcatcg
     2161 ttcttattgc aatttgtatc tcgataggat gcaatcagga ccatggtata tcacaaatag
     2221 acctttacga tcgcaacgat aaggcacaac aaatttcttt tgacaattat gatgcaactg
     2281 tcattacatt tctgggacct gaatgtccat tgagtgaaaa ttacacgaag acaatcaatg
     2341 acctggatag tacttttaaa agtaatgtgc atttcatcaa tatttttccg ggtacatttt
     2401 atagcaagga agctatcgac agttttatgc atacttacaa cgtaatgcag aattgcttat
     2461 tagatgccga ttttgatctc gttcgtgaat tgcacgcaac cattacacct gaatcatttg
     2521 tgttcgacag gaatggaaaa ctagtgtata gcggagcgat agataattgg gctgttgacc
     2581 tgggacaaaa gcggcaggta atcactgagt tttatgttcg cgatgtattg aatgccatgc
     2641 tgcaacataa agaaattccg tataccaaaa ctacagctgt aggatgtttt attgaaacag
     2701 aagcaaaaca acatcattga acatgcgcaa tctcatacta tttgcttttc ttgcattgtt
     2761 gatgacgtca tgcgccgata aaagtggttc aaacaaaaaa caggcggatg aagaaaaagt
     2821 aatattgccg gatgtagtta ctttcaatga acacattgca ccaatcatat tcgaaaactg
     2881 caccccgtgt catcgcccac atagtgcagg tccatttcca tttactacgt atgatgaaat
     2941 aaaagcaaag gcaaaaacaa tttcaaaagt aacccaatcg cgcttcatgc cgccttggcc
     3001 tgcggatcct agttacacta atttcaaagg tgaacgcatc ttaacagtta cagaaattgc
     3061 gatgataaaa aaatgggtgg aagatgattg ccctgtgggt gatgttgcga aaattcctgc
     3121 tgttccacat tatcctgaag gttcacaaat aggcacacct gatctggtga tcaattttcc
     3181 ggatttcaca ctggaaggaa ataataaaga tcatttcttt ttggtgaagg tacctttcga
     3241 aatacctgaa gacaaatatg tgaaaaccat tgagttcatc ccgggaaaca agaaacttgc
     3301 acatcacatg aatgcaaacc tgattgaata tcaaccggga aaaaagaaaa cagtattcaa
     3361 tggtaaaaaa attattccga caaatttagc tgagagtgcc gatgtagtgc aaactgaaat
     3421 ggataataaa aatgatgatg gcacctatcc ggtattagtt cctttggtat gcaattatct
     3481 ccccggtgtt tctccggcaa tgtatccgga aggcataggc ggtttcagga tgacaaagca
     3541 aggaacattt tttatcaacg acttccattt cggacctact ccgaaagacg caatagacac
     3601 ttcatcgcat tttaatattt tcttcgacga caaaccacct gtgcgaccaa cctatgaaat
     3661 attattaggt tcactcggtg tggctcctgt aactccggca ttggtaattc cgccaaatga
     3721 agtaaaaact tttactatcg attatcaagt acaggaaacc atgagtttgc tcaccgtaaa
     3781 tccgcatatg catcttttgg ggaaatcatt tttagcatat gcgcttaccc ctcagatgga
     3841 taccattcca ttgatacgta tcaataaatg ggattttcgc tggcagtatt tttatacatt
     3901 taaaagcgtg gtggtgatac ccggcggctc tcgtattcat gtagaagctg tatttgataa
     3961 taccgtgaat aatgtcaaca atccttatta tcctccacag gtgattattg acagaggcga
     4021 tagatttgat tcgatgaaaa ctaccaacga aatgttgcaa ttgatcctca catacatgga
     4081 gtatcaatcg ggcgacgaat acttgagcct cgaataacat gcgaaaatta tttccatata
     4141 tcacacgacc aattctggta ctgagccttg ttagtctctt taccgatatg gcgagtgaaa
     4201 tgctttatcc tgtcatgcct gcatttcttg aaagcatagg attcagcata ctgctgatag
     4261 gattattgga aggccttgct gaaatgatag cgggacttag taaaggatat tttggcaaac
     4321 gctcggatat tcaacaaaaa agattgccct ttgtccaatt aggttatgca ttaagtgcca
     4381 tttccaaacc catgatggca ttactcagct atccgctttg gatatttttt gcgagaacga
     4441 ccgaccgcgt tggaaaaggt ttgcgttcgg caccgcgcga tgcgatgttg aatgcaaatg
     4501 cgtcgaagga aacaaaagcc agcgtattcg gattccatcg ctccatggat accattggtg
     4561 ctattgtcgg acctttactc gccttgatat atctgcaatg ctatcctcaa gattataaga
     4621 atttatttct tctggcattt attcccggat tgatagcggt aatatttact tggtcactca
     4681 aggaagaaaa aaaagcggca agaggtgaac gcgtaaaatt tttttctttt ctctcctatt
     4741 taccgaatgc aaaaaaacca tatcgtcagt tggtaatcgg tctattggca tttgccttgg
     4801 taaatagttc cgatgtgtta ttactcttgc gcatgaaaca aatcggtatt gatgacgtga
     4861 ccattatatc catgtatatc ggatataata tcatttatgc attattggca tttcccattg
     4921 gtatattggc tgataaggta agcatcaaat acattttcat cgccggctta ttgattttcg
     4981 cagttgttta taccggattt gcatacgccg aatctgcaac cacatactat atactatttg
     5041 cttgctatgg catatatgcc gctgcaacag agggtatagc caaggcatgg atcaccaata
     5101 tcacacccaa ggaagaggca ggaactgcta taggcaccta cacaggatta agtagtattg
     5161 cagccttctt cgcatcagcg ctcgccggac tgatctggag tgttgcagga gcgggatggg
     5221 cctttggaat cagtgctgcc gtggctttac tggtaggtgc ataccttgtt agcatcccat
     5281 ttcaacgtgt tgtcagcagc gctaacggag cgtaaaagtg cgtatcttag ccccctaaac
     5341 atgccaccgc cgtatggatg tcagcccgaa acgctgcata actgtttcaa tcgccggaag
     5401 taagatggat aggtaagaac aatgtcgctt tacaaaaaga tcttgaacgg aaattggact
     5461 acaaccatta acctcgccat tcggggtgct accatcattg gtaaattcct gctggtttat
     5521 tttctcgcaa aaacacttac cgttgagcaa tatggtgttt ggggtatctt tcaaacttct
     5581 atcagcttat cactctacgt agtaggtctc gatttttata cttattcatc gcgccgcatc
     5641 ctcgattatc cggttgaaga acgcagccct atgttgcgcg atcagctcgt attttatttt
     5701 atcagctatc tcgtgatttt tccaattctc ggattgctgt ttgtattcaa tgaaatagat
     5761 tggcgatttg caattttctt ttacgccatt ctcctgcttg aacatttagc ccaggagacg
     5821 tatcgtacat tcgtagtttt cagcaaaccc atcattgcga atattgtatt gttcctgcgc
     5881 accggattat ggccatttgt actgttattc atgtggttca ttggcttcga tgaattgcgc
     5941 aacctcaaag cagtttacct gttctggatg gccggagggc tctctgctgc tgcagtttca
     6001 atttacttca tatctaaaat aaaatttaaa ccggtcaaaa atattcccat cgactggaaa
     6061 tggataaaac aaggtattgg cgtaagtctc atattttttg tcggaacagt aggatacaaa
     6121 atcattgaat ttgccgaccg ttattttgta gattattatc atagcaaaga ggatgtcgga
     6181 gtttatacct tctatgccgg tatatgtaat atcgttgaaa cttttgtgca tactgcggtg
     6241 atcattattt tctctcccaa gctgattgag acatttcata aagacaatta tcaatatcgc
     6301 aaaacctttg gcaccttcgc ccgtcaggtg gtaggcgcta ctttgctggt tggggtttca
     6361 ctggcattgt tgggaatacc attgctgcgc tctttaggtg aatcagaata catcgaagat
     6421 tataatgttt tcctactgct ggtattatca aaaatggtat tgaatatctc cctgatattc
     6481 cattacatct tgtatgtgag gaaaaacgat ttccctatta tcaaggctac catttatgcc
     6541 agcgtgatca atatattact caacttcatt ctcataccac cactaagcat catgggtgcc
     6601 gccatagcaa cactagtcag tttcatgatt atattggtga tgaaagttta ttacacccga
     6661 catcttccgg aagcacggca gatcatatac ctccgctttt taagacgaaa actgcgtaga
     6721 aaagcaaata caaaccatta atacgatctc gtatggagaa agcctattat gaacatatcc
     6781 aatggtggga ttacctgata tatctggtct acaatggcct gcttgcctac ctgttgggtc
     6841 gcgttggcga aatcgcactt aatatcggca aacaagcaag ggtgttgttc ttcatcgcat
     6901 ttgaagtgta tttctttttg gtgataggag attcgctgtt tcattacctt ccttaccttc
     6961 cggattcgga attgtatagt tatatgatct ccagcggtca atatcctgaa accagttccg
     7021 aaaacctgat tgtaatttat tatatctcca tttttcttag catcatctgt ctcaacgatc
     7081 ctgttatatt taccttcttc tgcatttttc tgtatgcact tggggcgatg ttatttattc
     7141 aggcatggaa aaaagctgat ccctcattta atactaacag tgaaacaagt gcagcgctgc
     7201 tctttttgtt gtggcctgca gcagcattgt atatcaccac tccattgagg gaagcattta
     7261 ttatgttcgg ttttggattg ttcctcaacg gatttatgga ttgcatctac aaaggaaaat
     7321 ggcgctctgt gattatcggc tccatcatta tttgcgctat tcgactgcaa ttatttgtat
     7381 ttgtgctgcc tgttttaggc gcactcgttg tctacaaatc atcactccat aaaatctgga
     7441 aaagcgctat cctcgtgggc acgatagtag cagcattttt agtggtgcgc tatgtattgg
     7501 ttgaacatcc actcagtccg caaacaatgg ctcaattgcg caatgtaaat cttgataacg
     7561 ccggaccatt gggatacggt cgcgttgatt gggatagcta taccgacatg gcccgagact
     7621 atcccttcat cattgcacaa ttcatgttta gtcccttccc tattttcgtg caacatgatc
     7681 cattgtcgac atttgtccct tttctagatt gcttattttt gatatttttg ttgatattga
     7741 ttttttcgag aattaaaaca ctcatccgtc agcatctacc ggtgctttta ctctgtgttt
     7801 tttatatcat tttgttcggc ggatacgaat atcatatcac aggagccgta cgacaccgta
     7861 tgcccttgga agcattattc attttaatgg cagcacctgc attgagccga attatattca
     7921 gaaagaaaca tgccgcagct tagcatactc ctttcaacaa ttaacgatag gatatttcag
     7981 gcagaaacta ttctcaatac ccctttgcct gacaccgagt ttgttgttgt gcatcagctc
     8041 actgaaggaa atgtagcaga aaattatcag tcattctatg cgcgttttac cgaaaagcct
     8101 gttcgattta ttcaacaatc ggcaaaagga acaggtcgca gcagaaatgc agcactcagg
     8161 gcaggaagcg gcaatttatt ttatttgtgc gacgacgatc tgatattgga acctgagttt
     8221 ctcgatcgta ttttgcaagc ctcatcacaa tatccaaacg ctgatatttt tacttttcaa
     8281 attcgcacta ccgaaggtaa accatacaaa aattatcccg acaaacccta tcaacacacc
     8341 ttgcgcagca ctgccaccgt ttcaatagtt gaaatggtga tcagaaaatc tgctgtcgac
     8401 agtgaaatga ttgccttcga tgagcgcttt ggcttagcaa cagattataa taccggtgag
     8461 gaatttgtga tgttacacga tgccttaaca aaaggtgctg ttgcgatgta cattcctcag
     8521 gtattggtgc aacatcctcc tgtcagctca ggaaaaattt ataataaaac cgtggcattc
     8581 gaaaaaggag cgatgattgc acgtgtgtat gattggcgat ggccgttcat taatgtggca
     8641 tttgctgtgc gcaagtataa agaatatcga cattcagcag gatttttcgc atttttgatt
     8701 gccatgtaca aaggcagtat gcatttcaac aaaaatgact gaaccaacgg tttccgttat
     8761 catgcctgtt tacaacgcag cgaaatatct gcaacaggcc gttgactctg tcaaatcgca
     8821 gacctatgcc aattgggaat tgtggatggt gaatgataaa agcactgaca actccgcaga
     8881 aatcatgtcg caactggcct ccggcgatgc acgtattcac gcgattcatc tcgaaaataa
     8941 tagtggttct gcacatgcca gaaatattgc catcgcgcaa gcaaagggaa aatacattgc
     9001 ttttttagat gccgatgatc aatggaaacc cgaaaaatta aaattacagg tggcattcat
     9061 gcaacaacac aatgctgtac tttcacatac tgcctacgat accatttctg cttcaaacga
     9121 atctctgcat cgcgaaattc atgtcaatgc aaaggttacc tatcgtcaat tattaaaaaa
     9181 taataccatc ggatgcctca ctgccatgta taatgcagag gccttaggaa aacaatttat
     9241 gcccgatctg cgcaaacgtc aggattatgg cttatggttg caaatattgc ggaatggacg
     9301 agtagcttac ggtctcgatg aagtgcttgc cacctatcga gtaggtatgg acagcctttc
     9361 cggcaacaaa tggcgtgtgc tgcgatacaa ttggatgctg ttgcgcaaac atgaacaatt
     9421 atcattaatt tcatccacgt attactttct ttgttttctg tggaataaga catttaaata
     9481 tttaggcaaa tgaaacgtgc aataattttc ggatccagtg gcttgacagg aaatgcactt
     9541 gcaaaattga tagcagaaga ccctcgctat gatgaattaa tttgctttca acgcagagag
     9601 gtcgccattc aatatcctaa acaaaaaaat gtgattacca catttcagga tttacattat
     9661 tatgccgact tgttttatgg cactgatgta ttctgttgcc tcggaacaac catgcgaaat
     9721 gtcaataatg ataaagttgc ttatcgcgaa gccgatgtcg accgaccact agccgctgca
     9781 aaagccgcag cttcgcatgg cgcaactagt tttacaatac aaactgcact tacggcaaat
     9841 gccaaatcga tggtatttta caacaggata aaaggtgagg ttgaagacgc tgtcaggaat
     9901 cttggtttgc gtgctgttta tatatatcgt ccaagcattc tcaccggaaa ccgacaagaa
     9961 aaaagaacag gcgaaggcat gggaatcacc atcaccaaat ttgtgactcc aatacttgtt
    10021 ggtccctgga aaaaataccg cgcaatcgct gccgctgatg tggcaaaagc tatgttgata
    10081 gtggcaaaat cagaaaaacc aggcgatcat atttttcttt ccgatcgcat tcaagcaatc
    10141 gccgatggca gcacgtggca atgaaaatta tacccaattt gtaaaacaac tttctgctga
    10201 attaggattt agctactgcg gcatttccac tgcgacagaa ctcaccgaag aagcccatca
    10261 gcttgaaaaa tggttgtcga aaggaatgca cggcgccatg ggctatatgg ctgagcattt
    10321 cgacaagcgc attgacccgc ggaaacttgt gccgggagcg cgctctgtta tatcattgat
    10381 gtataattat
//
LOCUS       DAOQOU010000118        10363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3375_length_10363_cov_6.109527, whole genome shotgun sequence.
ACCESSION   DAOQOU010000118 DAOQOU010000000
VERSION     DAOQOU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_10363_cov_6.109527"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1342)
                     /locus_tag="PLM90_10330"
     CDS             complement(176..1342)
                     /locus_tag="PLM90_10330"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HMZ89815.1"
                     /translation="MKPWLLVIVIFLLFVVELLRIWFIMPFPGSQEGGTLRLAYFMSN
                     NIWWMRLLLYIITAQFLFRFILRRKVFAAIIVFSMLIIYGVMTYYLVYKLNASAMFKQ
                     QQNPVYATAANNSVNEGKLVIGVVINGEAKAYPIEIIGYHHQVRDVIGRQELMVTYCT
                     VCRSGRVFSPVVNGNITNFSLVGMDKFNAMFEDEETGSWWQQATGIAIAGKMKGTALP
                     EIYSRQMSLHAWLTEFPDSKILQPDDNFLKQYNQLTGYDEGKVNNDLEHRDPLPWQNK
                     SWVIVAKVGEHKRAYDYAELEKRHVINDTLGGKEIAVTLALDNKSFYAFERRNDDVVY
                     QFFYDSKFTNGLGDNNSNLTWSFSGQARLSEDSTIQLRQIPVYQEFYHTFKDFE"
     gene            1435..3099
                     /gene="recN"
                     /locus_tag="PLM90_10335"
     CDS             1435..3099
                     /gene="recN"
                     /locus_tag="PLM90_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMZ89816.1"
                     /translation="MLRSLHISNYAIINEARIGFGEGMHIITGETGAGKSILLGALGL
                     ILGERADSRVLYQKDKKCVVEGTFSIADYGLKSFFTDNNLDYEEETIIRRELSDNGKS
                     RAFVNDTPVNVQVLQQLASKLITIHSQHETLDLGNSAFQLTVIDALAQTGELLTTHKQ
                     AFEAWHLCQQQLEDTISLASRAAQERDFIQFQFEELDKAGLDAIDQEALEAELTQLTH
                     AEEIKRNLMAAVDLMDQGQANIADQLRAAISQLRSVEKYLPTLESYLQRLSSAQIELQ
                     DISRDLDDLASGTYADPSRSEIINQQLSAVYRLQKKHQCADTQALIALRDDLGQKLMV
                     FEQSESRIEALTKETSLLLAKANQSASKLSAKRKAVFASFEKQVKALLAEVGMKDAEI
                     KVSHTFDADRHLTINGADQIQFLFSANKGAALQDIKKTASGGELSRLMLCIKSLLAKS
                     VALPSLIFDEIDTGVSGEVAQKVGNILVRLADNHQVIAITHLPQIASKGAHHLLVYKS
                     NEGGSTHTHIRSLQAEERILEIAKMLSGEKPTKAAIENAKELMLHQ"
     gene            complement(3163..6303)
                     /locus_tag="PLM90_10340"
     CDS             complement(3163..6303)
                     /locus_tag="PLM90_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89817.1"
                     /translation="MNRFSFLLATLTTLFITSISAQITPTAKKPWPAMPNSPNYYEVR
                     QAYLDHVANEEMETELLGLEAEDDDVYAKFNRWDYLMKTRVDANGNYPDPRTAFLEWE
                     AYKKSHAEAYEGQRAAGWEPVGNAEVPTDGGGVGRVNVIIQDPIDAGVLYVGTAGGGL
                     WKSPDGGTTWLPLTDNIPVTSIADVVVDYTNNDIIYVVSGDGYGYEASWQADNDFWGG
                     VYSAGILKSTDGGVTWLPTGLSYNQDELMIVQRLVMNPADPNILVAATREGMFRSTDG
                     GDTWTLVSSVHCYDFAQHVTDSDILYAVGDRDVMRSTNAGASWTVLKNNLAGTDDRMS
                     IETSNDNPDKIYVLSYGGQMDISDNGGTTWSSMSSPSGATSFYNYYDLVLDVSNVDEN
                     LIFAGGLEIARSTNGGTSWSKKSVWDDWTASNYVHADSKGLLCDPNDVNIVYATNDGG
                     VFKSTDKGQTWTDISAGLRVAQPYRLSPAYTVTDMVLNGWQDNGCNLWDGTTWKRVQG
                     GDGMDVIIDYSNEDRMYASFQYGYVNRTTNGGISWTPLPVAGGGWLTPYEMDPVDHMI
                     MYYGASTGTIQKSLNGGTSWSNLSSNLGGEVFDIAIAPSNTNYVYACALQTMKVSTNA
                     GTTWTNITSGLPTAGTGFNYIAVSDEDPTHVWVAISGYEDGKKVYFSDNAGSTWTNVS
                     GTLPNVPVNCIVYENVSDDDRVYIGTDIGVFTRTNATDWEPYMTGLPNVMVHELEIDY
                     AEYKIYAATYGRSTWKSDLYDFVAPTLNVGVVDLQYCPGQDVDVPYVGTGTFAAGNVF
                     TAELSNAAGSFTTPTAIGTFASTDLAGNIPSVIPTTASGAGYRIRVKSSSPGLTSTDN
                     GANILIDCEQPVDVATDVVTPTTATLDWADVYCAIEYEVRYKSSIDVDYTYVTTTSST
                     YTLTDLNPSTPYEWAVRTICLASPDVSTDFTSSEDFTTGQNAITDLIGVSGIFLYPNP
                     ASDNCALQLNTANAMDMRIELFDVTGQSVVILQNGTIAAGDHSIALDLAHIAAGAYTL
                     KISANGKSAALQLIKE"
     gene            6456..8609
                     /locus_tag="PLM90_10345"
     CDS             6456..8609
                     /locus_tag="PLM90_10345"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMZ89818.1"
                     /translation="MKKLLLILLLILMRISVYADGGMWLPILLEQLNIADMQQRGFQL
                     TAEDIYSVNKSSMKDAVVLFGGGCTGEVISEEGLVLTNHHCGFSSINALSSLEHNYLR
                     DGFWAKNKSEEIPAPGLTVTFIISMHDVTDSIIPYLNPQMDEQTRANVVSQRSAQLSA
                     AFKKGTHYDAMVRSFYYGNAYYLFVTETFRDIRLVGAPPASVGNFGGETDNWVWPRHT
                     GDFSMFRIYAGKDNKPADYNAENVPFKPRYSFPISLKGVEANDFTLVYGFPGRTQEYL
                     TSYAVDLTVNSFNPNRISCRDARLEIMNDYMRGNDTITLQYASKAKGLANAYKKWKGE
                     MLGLQQTDAIGIKQQYETDFQVWANTHVGKDYRYVLDNLKQAYTSFRPYSELADYTSE
                     AFYAVEAINFAGNFRSLVEKSAVDSITNEALGQIAAELNNKSEGFYKNYCLRLDRDMF
                     AVMMEIYSQKVDKQLQSDYFKSQVAAYHGDFKAWANAVYGKSVFTHPDKLNKLLSTYS
                     KKKLKKLSNDPLYKLYIEVTSLYEVQSKQFIQPAQIQIATLMRDYMRGQMAKDNEKIF
                     YPDANSTLRVTYGNVEGYTAKDAVYYNFQTTDRGLLEKYKEGDEEFDLPKPLVDLIKA
                     RNFGAYGDVNGNLPIAFIASNHTTGGNSGSPVINAKGELIGTNYDRVWEGTMSDIMYD
                     VDRCRNISLDIRYTLFIVDKYAGATNIIQELKIVD"
     gene            complement(8610..>10363)
                     /locus_tag="PLM90_10350"
     CDS             complement(8610..>10363)
                     /locus_tag="PLM90_10350"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HMZ89819.1"
                     /translation="SSLRFEEAEQVTMGIRVARLIKRKNELITWPPCDPNTEHVWTNI
                     SFAKPIVFEGLQGGHPFYISPYAIASYLEFQELNAAGTAYEKQAIFMDRKFYVDNEIA
                     DKIISNIGLDVKYGISKNLTLDLTVNTDFAQAEVDDRIINLSKYEVNLPEKRTFFLES
                     ANALSFGFPSGNELFITRRIGNEHGQIVPILAGARITGKANGWLIGALNMQTTGLVTP
                     YDTISPHNFTVLRTRKDIDKLGSFVGGIICNRIDTDTSGKSYQAIGLDIVKRITQQFT
                     IEGGIANTMADLNAENFADGLYSHVGVFKSATSGFTYHSLLDIVGKDVNPVMGYLDEH
                     DYGAWYPQIQYQWDLQKSDLLQYFYISADYGYRWKLSSGDRETTGGSLATGVIFENGA
                     SVEWEPLSIKKDSLFFDWELDAGNAISAGNYLMYNTILDVVLPTQSTYNISLGGTYGG
                     FYGGDRAFAYVNLQYYISSHLNANLTYEFNDIQFDQYLDIDSATSYMSNLVRLGVNYN
                     FSTKVSLKAYIQYEDISDMTSANLRFRYNPQEGTDLYIVLNQGLNNDRDRLDPKLPYA
                     QSQAVTIKFTKTFAVKT"
BASE COUNT         3123 a         2138 c         2094 g         3008 t
ORIGIN      
        1 ggtagtgagt agtgggtatt gagtagtgag actaatgcaa ttattcgctc gcaaaagcga
       61 gcgaactgag ctcaccaccc gccggaggcg aggcaagctc acccactcac ccactgaccc
      121 actcacccac tcacccactc tctccttgct cctctttcct ttctcctctc tcctctcatt
      181 caaagtcctt gaaggtatgg taaaattcct ggtaaaccgg aatttgtctg agttgaatag
      241 ttgagtcttc actcaatctt gcttgtccgg aaaaagacca tgtgagattg gaattattat
      301 cgccaagacc gtttgtaaat ttactatcgt agaaaaattg ataaactacg tcatcatttc
      361 tacgttcaaa ggcgtagaat gatttattat cgagtgcgag cgttaccgca atttcttttc
      421 cgcccaaggt atcgttgatc acatgtcttt tctcaagttc agcataatca taagcacgtt
      481 tatgctcacc aacttttgct acgatgaccc atgatttatt ttgccatggc aagggatcgc
      541 gatgttcgag gtcattgttt acttttcctt catcataacc tgtgagttgg ttgtattgct
      601 ttagaaaatt atcatcaggt tgaagaattt ttgaatcagg aaattctgtc agccaggcgt
      661 gtaatgacat ttgtcggctg taaatttccg gtaatgctgt tcccttcatc ttacctgcaa
      721 tagcaatacc ggtagcttgt tgccaccatg aaccggtttc ttcatcttca aacatggcat
      781 tgaatttatc catgcccact aaagaaaaat tagtgatgtt accattcaca acaggactaa
      841 aaacacgacc gcttctgcat actgtgcaat aggttaccat caattcttgt cgcccaatga
      901 catcgcgtac ctgatgatga taaccgataa tttcgattgg atatgctttt gcttcgccat
      961 tgattactac gcctattacg agttttcctt cattcacact attatttgca gcagttgcat
     1021 aaacaggatt ttgctgctgt ttgaacatgg cggaggcatt taatttatat accaggtaat
     1081 aggtcatcac gccatatatt atcagcatag aaaatactat gattgcagca aatactttcc
     1141 ttctcaaaat aaaacgaaat agaaactgcg ctgtgatgat atacaggagc aatcgcatcc
     1201 accatatatt attgctcatg aaatatgcga gtctaagcgt gccaccctcc tggcttccgg
     1261 ggaatggcat gataaaccag atacggagta attcaaccac aaataagagg aaaatgacaa
     1321 taacgagcag ccatggcttc atgctacaaa gatagaaagg catgtgaaaa tggagtgtaa
     1381 aactcctgta aaggcaaacg ctcatgtgca tgcagtttgc attatctttg aaccatgttg
     1441 cgcagtctgc acatatcaaa ctatgccatt atcaacgaag ctcgtatcgg gtttggcgaa
     1501 ggcatgcata ttattacagg agaaaccggt gccggtaaat caattttgtt gggggctttg
     1561 ggattgatat taggcgaacg tgcagacagc agagtgttat atcagaaaga taaaaaatgt
     1621 gttgttgaag gcactttttc aattgcagat tatggattga aatcattctt cacagataat
     1681 aatctggatt atgaggagga aaccattatt cgcagagaat taagtgacaa cgggaaatca
     1741 agagcgtttg taaacgatac tcctgtaaat gtgcaggtat tacaacaatt agcttcgaag
     1801 ttaatcacca ttcattcgca acatgaaaca cttgatttag ggaattctgc ctttcagtta
     1861 actgtgattg atgcattggc acaaacagga gaattactta ctacgcataa acaagcattt
     1921 gaagcatggc acttgtgtca gcagcagttg gaagatacca tttcactggc atcacgcgct
     1981 gcacaggaac gcgattttat tcagtttcag tttgaggagc ttgataaggc cggactagat
     2041 gccattgatc aggaggcgtt ggaggccgaa ttaacgcaat tgacacatgc tgaggagatc
     2101 aagcgcaatt tgatggcggc tgttgattta atggatcaag ggcaagctaa tattgctgat
     2161 caactgcgtg ctgcgataag tcagttgcgc agtgtagaaa aatatctgcc aacattagaa
     2221 tcgtacttac aacgactttc atctgcacag attgaattac aggatatttc ccgcgacctt
     2281 gatgatcttg catcaggaac atatgcagat ccatcgagaa gtgaaattat caatcagcag
     2341 ttaagtgcgg tttacagatt gcaaaagaag catcagtgtg ccgacacaca agctttaatt
     2401 gcattgcgtg atgacttagg tcagaagctg atggtttttg aacagagtga atcgcgtatt
     2461 gaagcgttga ccaaagaaac tagtttactg ctcgccaaag cgaatcaaag cgcgtccaaa
     2521 ttatcagcaa aacgcaaagc tgtttttgca tctttcgaaa aacaagttaa agccttgtta
     2581 gcggaggtag gtatgaaaga tgcagaaatc aaagtttcgc atacatttga tgcagatcgt
     2641 catcttacca ttaatggcgc agaccaaatt caatttttat tttcagccaa taaaggagcc
     2701 gcacttcagg atatcaaaaa aaccgccagt ggaggggaat tatctcgttt gatgctatgc
     2761 attaaatcgc tcctggcaaa aagtgtagca ttaccttcac taatattcga tgagattgat
     2821 accggagtat ccggggaggt tgcgcaaaag gttggtaata ttcttgtgcg actagctgat
     2881 aatcaccagg taattgctat cacacatttg ccgcaaatag cttcaaaagg tgcacatcat
     2941 ttattggtgt ataaatctaa tgaaggtggc agtacacata cacatatcag gagcttacag
     3001 gctgaggaac gcatactgga aattgcaaaa atgctgagtg gcgagaagcc tacaaaggcg
     3061 gccattgaaa atgccaaaga gctcatgctt catcaatgaa ttcaatcaat aaaaaaggac
     3121 ctgcaataaa gcaagtcctt ttgaaataat ttcagtttaa atttattcct ttatcaattg
     3181 caatgctgct gattttccgt tagcagagat tttgagcgta tatgcacctg ctgcaatatg
     3241 agcaagatct aacgcaattg aatgatctcc ggcagcaata gttccatttt gcaatatcac
     3301 tacagattga ccggtaacat caaataattc gatgcgcata tccatggcgt ttgcagtatt
     3361 taattgtaat gcgcaattat cacttgccgg attcggataa aggaagatgc cacttactcc
     3421 gatgagatca gtgatggcat tttgtcctgt agtaaaatct tcgctacttg tgaaatcggt
     3481 agacacatcc ggtgaagcaa gacatatggt acgcacagcc cactcgtatg gtgtgcttgg
     3541 attaaggtcg gtaagcgtgt aagtcgaaga tgttgttgtt acataagtat aatcaacatc
     3601 tattgatgat ttatatcgca cttcatactc aattgcgcaa tatacatctg cccaatcgag
     3661 tgttgctgtt gttggtgtaa ccacatcagt agcaacatca accggttgct cgcaatctat
     3721 taaaatattt gcgccattgt cggtgctggt aaggccagga gaacttgatt tcactcgaat
     3781 acggtagcct gcgccactgg cagtagttgg aataacagat ggaatatttc cggcaagatc
     3841 agttgaggca aaagtgccaa ttgcagttgg cgtagtgaaa ctaccggcag cgttacttaa
     3901 ttccgctgta aatacgtttc cggctgcgaa ggttccggtt cctacatatg gtacatcaac
     3961 atcctgaccc gggcaatatt gcaggtctac aacacctaca ttgagtgtcg gagcaacaaa
     4021 atcatacaaa tctgacttcc atgtactgcg accataagtt gctgcataaa ttttatactc
     4081 ggcataatct atttccaatt cgtgcaccat cacattgggt aagccggtca tgtatggttc
     4141 ccagtcagta gcatttgtgc gggtaaacac gccaatatct gttccgatat acacgcgatc
     4201 atcatcactg acattttcat aaacaatgca attaacaggg acatttggca gcgttccgga
     4261 gacatttgtc catgttgaac cggcattatc agaaaaatat actttcttgc catcttcata
     4321 tccgctgata gcaacccaaa catgtgttgg atcttcatca ctaacagcaa tataattaaa
     4381 ccctgtacct gcagttggta gtccggatgt gatatttgtc catgtagttc ctgcgttggt
     4441 cgacaccttc atagtttgca atgcacaagc ataaacataa ttcgtatttg agggtgcgat
     4501 ggcaatatca aatacttcac caccaagatt tgaagaaagg tttgaccatg aggttccacc
     4561 atttaatgat ttttgaatag tacctgttga agcaccgtaa tacatgatca tatggtcaac
     4621 gggatccatt tcataaggag tcaaccaacc acctcctgca accggaagtg gagtccagga
     4681 aattccaccg ttcgttgtac gatttacata tccatattgg aaagaggcat acatacgatc
     4741 ttcattggaa taatcgatga ttacatccat tccatcgcct ccttgaacgc gtttccatgt
     4801 tgtaccgtcc cataaattgc aaccattatc ctgccatccg ttgagcacca tgtctgttac
     4861 ggtataagca ggagataaac gataaggttg agctacacgg agacctgcgc tgatatcagt
     4921 ccaggtttga cctttatcgg tagatttaaa tacacctcca tcattcgtgg cataaacgat
     4981 atttacatca ttaggatcac agagtaatcc tttggaatct gcatgtacat aatttgatgc
     5041 tgtccaatcg tcccatacag attttttcga ccagcttgta cctccgtttg tcgacctggc
     5101 aatttctaat ccgcctgcga aaatgagatt ttcatctaca ttcgaaacat cgagaacaag
     5161 atcataataa ttatagaaag atgtagctcc tgaaggagaa ctcatagatg accaggttgt
     5221 accgccatta tcggagatat ccatttgacc accataagat agcacatata ttttatcagg
     5281 attgtcgtta gatgtttcaa tgctcatgcg atcatcagta cctgcaagat tatttttcaa
     5341 taccgtccat gaagcgcctg cattggtaga cctcattaca tcgcgatcgc caactgcata
     5401 taaaatatca ctatctgtaa catgctgcgc aaaatcatag caatgaacac tgcttaccaa
     5461 ggtccaggta tctccgccat cggttgaccg aaacatccct tcgcgagtcg ccgctactaa
     5521 aatgttagga tcagcaggat tcatcaccag acgttgcaca atcatcaatt catcttggtt
     5581 atagctcaat cctgttggca accatgttac accgccatca gtgcttttca aaattcctgc
     5641 gctatataca ccgccccaaa aatcgttgtc ggcctgccaa gaagcttcat agccatagcc
     5701 atcgcctgaa actacatata tgatatcatt attagtataa tcaacaacta catcagcaat
     5761 agaggtaacg gggatattat ctgtcaatgg cagccatgta gtgcccccat caggcgattt
     5821 ccataatcca cctcctgcag ttcctacata tagaacgccg gcatcaatag gatcttggat
     5881 aatcacgttc acacgtccaa caccaccacc atcggtcggc acttcagcat ttccaacggg
     5941 ctcccatccg gcagcacgct gaccttcata ggcctcagca tggctcttct tgtatgcttc
     6001 ccattccagg aatgccgttc tgggatcggg gtagtttccg ttggcatcta cccttgtttt
     6061 catcaggtag tcccagcgat taaattttgc atatacatca tcgtcttcag cctctaaacc
     6121 taataattca gtctccatct cttcattggc aacatgatcg agataggctt gacgaacttc
     6181 atagtaatta ggtgaattag gcattgcagg ccatggcttt tttgccgtag gcgtaatttg
     6241 cgccgaaata gacgtaataa acagtgtggt cagggttgcg aggaggaatg aaaatcggtt
     6301 catgcaatta cattatgtgt taaaattaca aaattttcgg cttaattttt ttctggatta
     6361 caacaatttg acaattcctc gaactagtat taacaagtca ttcacaaaca caaacacgaa
     6421 atcacgtaat tttgttgtca caaataatgc attttatgaa gaaattactg cttatcctct
     6481 tattgatttt gatgcgtata tcggtgtatg ccgatggagg catgtggtta ccgatattgt
     6541 tggagcagtt gaatattgcg gacatgcaac aacgtggatt tcagttaacc gctgaagaca
     6601 tttattcggt gaataaaagc agcatgaagg atgcagttgt tctattcgga ggaggatgta
     6661 cgggagaagt catctctgag gaaggacttg tgctcaccaa ccaccactgt ggtttttcaa
     6721 gtattaatgc cttgagttct cttgaacata attatttgag agatggattt tgggcaaaaa
     6781 ataaaagtga ggagatccca gctcccgggc taacagttac ttttatcatc agcatgcatg
     6841 atgtaactga tagcatcatc ccatatttaa atccgcaaat ggatgaacag actcgtgcta
     6901 atgtggttag tcaacgcagt gcacagctga gtgctgcatt taaaaaagga acacattatg
     6961 atgctatggt gcgttcgttt tattatggca atgcatatta tttatttgta acggaaacat
     7021 tccgcgacat cagacttgta ggagcacctc cggcatctgt cgggaatttt ggcggcgaaa
     7081 cagataattg ggtgtggcca agacataccg gcgacttcag catgtttcgc atttatgcgg
     7141 gtaaggacaa caagcctgca gattacaatg cagaaaatgt gccattcaaa cctcgatatt
     7201 catttcctat ttctttaaaa ggtgtggaag caaatgattt tacattagtg tatggttttc
     7261 cgggacgcac ccaagaatat ttaacttcat acgcagttga ccttactgta aatagcttca
     7321 atccaaatcg catttcatgt cgcgacgcac gactcgaaat tatgaatgat tacatgcgcg
     7381 gaaatgatac cattaccctg caatatgcgt caaaagcaaa gggcctcgcc aatgcataca
     7441 agaaatggaa aggcgagatg ttgggattgc agcaaacaga tgcaattggt atcaaacaac
     7501 aatatgaaac tgattttcag gtttgggcaa atacgcatgt tggaaaagat tatcggtatg
     7561 tactcgacaa tttgaaacag gcttatacat cctttcgacc ttattctgag ctagccgatt
     7621 acaccagtga agcattctat gctgtggaag ccatcaattt tgcagggaat ttcagaagtc
     7681 tggttgagaa atcagctgtc gacagtatta ctaatgaagc actggggcaa atagctgccg
     7741 aactaaacaa taagagcgaa gggttttata aaaattattg cttacgtctc gaccgcgata
     7801 tgtttgctgt aatgatggaa atttattcgc agaaggtgga taaacaatta caaagtgatt
     7861 atttcaaatc gcaagttgca gcatatcatg gagacttcaa ggcttgggcg aatgctgttt
     7921 atgggaagag tgtattcaca catcctgata aattgaataa gctgttatca acctattcta
     7981 aaaagaaatt aaagaaatta agtaatgacc ctttatataa attgtatatt gaggtaactt
     8041 cattatatga agtgcaatcc aaacagttca tacaaccagc tcaaattcaa atcgccactt
     8101 taatgcgcga ttacatgcgg gggcagatgg ccaaggacaa tgaaaagata ttttatccgg
     8161 atgccaattc gacattacgt gttacctatg gaaatgttga aggttataca gctaaagatg
     8221 ccgtttacta taattttcaa actactgatc gtggtctact ggaaaaatat aaagaaggcg
     8281 atgaagaatt cgatctacca aaaccactgg ttgacctcat caaggcgcgc aattttggtg
     8341 cctacggcga tgtgaatggc aatttgccga tagcatttat cgccagtaat catacaacag
     8401 gcggcaatag cggaagccct gttatcaatg cgaaaggtga actgattggc accaattacg
     8461 atcgcgtgtg ggaaggcact atgagcgata ttatgtatga tgtggaccgc tgtcgcaata
     8521 tttcactcga cataagatat accttattca tcgtcgataa atatgccggc gcaacgaata
     8581 ttattcagga attaaaaatt gtagattaat caggttttta cggcaaaggt cttggtgaat
     8641 ttaatggtaa cagcctgcga ttgcgcgtaa ggtaatttag gatcaagacg atctctatcg
     8701 ttattgagtc cttgattcaa tacaatatag agatctgttc cttcctgagg attatacctg
     8761 aatcgcaaat tggcgctggt catgtctgag atgtcttcgt attgaatata ggctttcaat
     8821 gaaacttttg tcgagaagtt gtagtttaca cctaatcgaa cgaggttact catatatgaa
     8881 gttgcactat caatatcaag gtattggtcg aattgaatgt cattaaattc ataagtaaga
     8941 ttcgcattca ggtgactgct gatataatat tgcagattaa cgtaagcaaa tgctctatct
     9001 ccaccataaa atccaccata ggtaccaccc agactaatat tatatgttga ttgcgtaggg
     9061 agcactacat cgagtatggt attatacatt aaataatttc cggcactaat ggcatttccg
     9121 gcatccagtt cccaatcaaa aaaaagtgaa tcctttttta tagaaagcgg ttcccactca
     9181 acagaagcgc cattttcaaa tatcacacct gttgcaagtg atcctcccgt agtttccctg
     9241 tcgcctgatg ataatttcca tcgatatcca taatcggcag aaatataaaa atattgcaag
     9301 agatcacttt tttgcaagtc ccattgatat tgtatttgcg gataccaagc tccgtaatca
     9361 tgttcatcga ggtagcccat tacagggtta acatctttgc cgacaatatc gagcaatgaa
     9421 tgataggtaa atcctgaagt tgcggattta aaaacgccga catgggaata caatccatca
     9481 gcaaaatttt cagcattcag atcagccata gtatttgcaa taccgccttc aatggtaaat
     9541 tgctgtgtta tgcgttttac tatatccaaa cctattgcct gataagattt tcctgaagta
     9601 tccgtatcaa ttctattaca aataataccg ccaacaaagc ttcctagctt gtctatatct
     9661 tttctggttc gcaatacagt gaaattgtgt ggtgaaatag tatcgtacgg cgttactagt
     9721 cctgttgttt gcatattcaa tgcaccaatc aaccaaccat ttgcttttcc tgtaatgcgt
     9781 gcacctgcca aaattggcac tatttgtcca tgttcgttac caattcttcg cgtaataaat
     9841 aattcattcc ccgaaggaaa tccaaaactg agtgcatttg cggattcaag gaaaaatgtt
     9901 cttttttcag gaaggtttac ttcatatttc gataaattga taatgcgatc atcaacttct
     9961 gcctgagcaa aatctgtatt tacagttaga tccagcgtaa gatttttact gatgccatat
    10021 tttacatcca atccgatatt gctgataatt ttatctgcaa tttcattatc gacataaaat
    10081 ttgcgatcca taaaaatcgc ttgtttttca taggctgtac ctgcagcatt taattcctga
    10141 aattcaagat aagatgcgat agcataggga ctgatataaa atggatgtcc gccctgcaaa
    10201 ccttcaaaaa cgattggctt ggcaaaagaa atattcgtcc atacatgttc tgtattcggg
    10261 tcacatggag gccaggtgat gagttcattt tttcttttga ttaatctggc aacgcgaata
    10321 cccattgtaa cctgttctgc ctcttcaaac cgaagtgacg aaa
//
LOCUS       DAOQOU010000119        10311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3404_length_10311_cov_5.650449, whole genome shotgun sequence.
ACCESSION   DAOQOU010000119 DAOQOU010000000
VERSION     DAOQOU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3404_length_10311_cov_5.650449"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..822)
                     /locus_tag="PLM90_10355"
     CDS             complement(49..822)
                     /locus_tag="PLM90_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ89820.1"
                     /translation="MGFIIGPALGGLLAGLGPRVPFYGAAILSLVNWLYGFFILPESL
                     PKEKRRKFEWKRANPIGSLAALKKYPLTYGLIASMIFIWLASHAVQSNWSYFTMGTFH
                     WDEKAVGFSLALVGVLVGLVQGLLIRVTIPRFGQKNSLYYGLLLYTIGLLLFAFATQG
                     WMMYAFLVPYCLGGIGMPALQGIMSSQVPANEQGELQGAMTSLMSVTSIIGPPLMTNT
                     YAYFTAKDTPYFFPGASFLLGAIFMAVSLLLTMRTLKKH"
     gene            complement(810..1256)
                     /locus_tag="PLM90_10360"
     CDS             complement(810..1256)
                     /locus_tag="PLM90_10360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ89821.1"
                     /translation="MSEKRQASMIFIFITMLIDVVGFGIIIPVLPNLIKELHGGDYSE
                     AARIGGWLLFAFSLMQFLFSPVLGNLSDQVGRRPVLLFSLFGFGIDYLIMAFAPSLWW
                     LVVGRVLAGITGASFSTASAYIADISTPENGRKILDLSALLLGWDS"
     gene            1302..2252
                     /locus_tag="PLM90_10365"
     CDS             1302..2252
                     /locus_tag="PLM90_10365"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HMZ89822.1"
                     /translation="MVTTPHNIKYQLGLTVNRALWVFSAATLLLLNSCAVLLPKEYRT
                     VGYNLEEPGFRKLLPPSLQEISGITQISMDTFACIQDEQGIVFIIDVTTGNIIKQMPF
                     FGDGDYEGIARVNNSLFILRSDGLLFEIPDFHSPEYGHITFKTGIPANNNEGLCYDPE
                     HNRLLIACKSNIGKGATLKNVRAIYAFDLGTKKLSSDPVFEVYISELQDYAKTHAMDL
                     PEKKSKHKKKQEQPDIKFRPSAIAIHPENHNIYLLSAVDHLLCIVNPNGSLVSLISLD
                     ENVFNKPEGIAFSSDNTMIITNEGQDKHPSLLSFPYKGRR"
     gene            complement(2253..4172)
                     /gene="acs"
                     /locus_tag="PLM90_10370"
     CDS             complement(2253..4172)
                     /gene="acs"
                     /locus_tag="PLM90_10370"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMZ89823.1"
                     /translation="MAVPYQITSAEAYQHAYAKSIENPAAFWADIASHFTWKKQWELT
                     LDWNFVEPHVRWFLGGQLNITENCIDRHLESLGNKPAIIWEPNDPEESHRVLTYRQLH
                     DKVCQFAHVLKNNGVEKGDRVCIYMGMIPELAIAVLACARVGAIHSVIFGGFSAQSIA
                     DRLQDAQAHFIITCDGAFRGAKDIPLKSVIDDALIACPFVERVIVCTRTRTPVSMLKG
                     RDVWWEDEIKKVETLGLHNFEPEVMESEDPLFILYTSGSTGKPKGVVHTTAGYMVYAN
                     YSFVNVFQYQPEEIHFCTADIGWITGHSYIVYGPLSAGATSLMFEGIPTWPDAGRFWD
                     IVEKYKVNILYTAPTAIRSLMGFGTAPIEGKDLSSLRVLGTVGEPINEEAWQWYHKHI
                     GKENCPIVDTWWQTETGGILISNLAGITEAKPSYATYPLPGIDPILVDENGNIIEGNN
                     VSGNLCIRHPWPGMLRTTYGDHERCRQNYFATYHQLYFTGDGALRDEAGRYRITGRVD
                     DVLNVSGHRIGTAEVENAINMHAGVVESAVVGYPHDIKGQGIYAFVVYTGGHQSDPQL
                     TRQDILLTVTRAIGALAKPDKIQFVSGLPKTRSGKIMRRILRKIAEGEPGQIGDTTTL
                     LDPNIVEEIKSGVIN"
     gene            complement(4212..5444)
                     /locus_tag="PLM90_10375"
     CDS             complement(4212..5444)
                     /locus_tag="PLM90_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HMZ89824.1"
                     /translation="MQKSNRLTLYILAAMVLGIFVGSMIHNSGKSDEQLGSIASKINI
                     TTTVFLSLIKMIIAPLVLSTLIVGIAKMGDVKTVGRVGGKTMLWFITATIISLTLGMF
                     LVNALQPGEAMNLALPAETASAGVDAGAITLEKFITHVFPTSIFKAMSENEILQIVVF
                     SIFFGVACAAIGEKAAPLIKALDIVGHVMIKITTYVMLFAPFAVFAAMCSVITTKGTS
                     VLLTYGIFMRDFYFGLLILWAILIIAGFAVLRKRVFILLRRIKEPFLLAFSTASSEAA
                     FPKLITELERFGCNNKIVSFTLPLGYSFNLDGSMMYMTFASIFIAQCYNIDLTMSQEI
                     TMLLTLMVTSKGIAAVPRASLVVIAGTISMFNIPQAGLVLLLGIDTFLDMGRSATNVI
                     GNAIATAAVSKWENALED"
     gene            5572..6216
                     /locus_tag="PLM90_10380"
     CDS             5572..6216
                     /locus_tag="PLM90_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ89825.1"
                     /translation="MAGKQNKLFLYGTLVVAALVVFAIFMVYNNTMSGNKELELPFYG
                     PRGSNGEPHYVQDFSLLNQDSVLLDMTPYKGKIYVADFFFTTCQGICPIMTEGMLKVY
                     HAYETTPNVMFLSHTVDPDHDTPYILKRYAGNRDINNAKWQFVTGDKKQIYDLARYSY
                     LVDASVGDGGPTDFVHTENFALVDPEGRIRGYYDGTDTVEVNKLIKDISLLMAE"
     gene            6313..7944
                     /locus_tag="PLM90_10385"
     CDS             6313..7944
                     /locus_tag="PLM90_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ89826.1"
                     /translation="MITVNNVSLRYGKRLLFEDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILAGEIEPTTGLIDITPGERMAVLKQNHNEFDQHSVIHTVMMGHRKLWNIIQEKDTL
                     YAKPDFNEEDGIRVGELEAEFAEMNGWNAESDAAELLSNLGVKEDLHYVPMEQISGKE
                     KVRVLIAQALFGNPDILLMDEPTNDLDIDTISWLEDFLADYENTVIVVSHDRHFLDAV
                     CTHVADIDYGKINIFTGNYSFWYQSSQLMLQLMAQKNKKVEDKRRELQEFISRFSANA
                     SKAKQATSRKKALEKLNVEEIRASSRKYPGIFFTPERDLGDQVLQVTNVSKSLEGRNL
                     FSNVNFTAEKGDKIAIVSRYPIAKTYFFEIIAGERQLDQGEFKWGVTVTSNYLPTDNS
                     SFFAGEMNLIDWLRQYSKEKDESYIRGFLGRMLFSGEESLKSCKVLSGGEKVRCMISR
                     AMLLGGNALIMDEPTNHLDLESIEALNNALADFKGFVIFTSHDHQFMQTIANRVIEIT
                     PNGIIDRLMTYDEYILDPKVKELQDQMYPSSVTAE"
     gene            8037..8576
                     /gene="msrB"
                     /locus_tag="PLM90_10390"
     CDS             8037..8576
                     /gene="msrB"
                     /locus_tag="PLM90_10390"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMZ89827.1"
                     /translation="MRTLIVVIALMPAIFGCSQNTQKATETNSSIETLDNKVTTESAD
                     YIFDGKIITKPDSVWQKELNGMTYYVMRQAGTERPFQNAYYDNHEKGIYYCAACGLPL
                     FSSETKFDSGTGWPSYYTPINDLNIGKDTDLDIGYVRTEVHCARCDGHLGHVFDDAPQ
                     TPTGLRYCINSASLVFKKQ"
     gene            8643..9197
                     /locus_tag="PLM90_10395"
     CDS             8643..9197
                     /locus_tag="PLM90_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ89828.1"
                     /translation="MYFHSIGRPFVGKKISAVLPFLPKDVPVLDIGSGNGLAASLMME
                     AGCKIVPIDIHEGQYHDSVKPTVYDGKHLPYVDNHFEAGVILTVLHHVDDPDALLKEI
                     MRVCKSLVIMEDIYENAFQKNITFWLDSLANLWYSPCPHTNKNDIGWKSLFNAMDLEL
                     TDVRYRNAFFVIKQAVYVVSKKSQ"
     gene            complement(9189..9971)
                     /locus_tag="PLM90_10400"
     CDS             complement(9189..9971)
                     /locus_tag="PLM90_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ89829.1"
                     /translation="MTILIQHIIKSTALCVGYDKHVILKDINIAVPQNSVTSIVGLNG
                     TGKSTLLRSLCGLQKILSGEVLLHNKSLSDFSAGEIAKQISIVLTGRGEMVSSLSVYE
                     LLAMSRTPYLHWLSGVSGHDIAIIEESAKVAGVSHLIDRSLYTLSDGEFQLVGIARAL
                     AQETPIIILDEPTAHLDMQNKLEVFSLLRKLSKSGKTILFTSHDIDLALAISDHVLFI
                     DKKGKVEFGETSSLIERNIPATYFDSANIQFDKETNRFKFLL"
     gene            complement(9968..>10311)
                     /locus_tag="PLM90_10405"
     CDS             complement(9968..>10311)
                     /locus_tag="PLM90_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HMZ89830.1"
                     /translation="YALSLGIHVPRLRLYIIVITGILTGTVTAFCGPIAFIGIAIPHL
                     ARMVLKVNDHRLLIPASVLFGMCVMLLCDMLTQVGGDAGVLPLNAVTAFMGAPFVIYI
                     IYKNQHLKRTL"
BASE COUNT         3205 a         2259 c         1961 g         2886 t
ORIGIN      
        1 cctaaaatta aagaccatct gtccaagtat cacgaatact ttgaatgatc aatgcttttt
       61 aagcgtacgc atagtgagca ataaactcac tgccataaaa attgcaccaa gtaaaaacga
      121 cgctccagga aaaaagtaag gcgtatcctt agcagtgaaa taggcgtaag tattggtcat
      181 caatggcgga cctatgatac tggttacact catcagactt gtcatcgctc cctgaagttc
      241 accttgttca tttgcaggca cctgtgacga catgataccc tgtaaagccg gcattccgat
      301 tccaccaagg caataaggca ccagaaaagc atacatcatc cagccttgtg ttgcaaatgc
      361 aaataataat agcccgatgg tgtagagtaa taaaccataa tacaaggaat ttttctggcc
      421 aaatcgaggt atagttactc tgatgaggag accttgaaca agacctacca aaacgccaac
      481 caaagccaga gaaaatccaa ccgctttttc atcccaatga aacgttccca ttgtgaaata
      541 actccagtta ctctgaaccg catgcgatgc caaccaaata aaaatcatac ttgcaatcag
      601 tccgtatgtc aacggatact tttttaatgc agccaatgat ccgattgggt ttgctctttt
      661 ccattcaaat tttctacgct tttcttttgg cagagactcc ggtaaaatga aaaagccata
      721 caaccagttc accaacgata atatggcagc accataaaaa ggcactctgg gacctaaacc
      781 cgcaagcaat ccacctaggg caggaccaat tatgaatccc atcccaaaag cagcgccgac
      841 aagtccaaaa ttttgcgccc gttttccggt gttgagatat ctgctatata agcactcgcc
      901 gtagaaaaac tggcaccagt tattcccgcc aataccctac caactaccag ccaccacaaa
      961 cttggtgcaa atgccatgat caggtaatct attccgaagc caaaaaggga aaataacagt
     1021 acagggcgac gaccaacctg atcactcagg ttaccaagaa cgggactaaa taaaaactgc
     1081 attaatgaga atgcaaatag cagccaccct cctatcctcg cagcttcgct gtaatcacct
     1141 ccgtgcagct ctttgattag attcggcaat acaggaataa taatgccaaa acccaccaca
     1201 tcaatcaaca tggtgatgaa tataaaaatc atggacgcct gccttttctc tgacattttc
     1261 aaatgttgag gcgcaaagtt atagatttgt agataaggaa tatggttacg actccgcaca
     1321 acataaaata tcaacttgga ctaacagtta accgggcact gtgggttttc agtgcagcaa
     1381 cattactgct tttgaatagt tgtgctgtat tacttccaaa agaatacaga accgtaggat
     1441 ataacctgga agaaccggga ttcagaaaat tactaccccc ttcgttgcag gaaatttcag
     1501 gaattaccca aataagcatg gacacctttg cctgtattca ggacgaacaa ggcatagtat
     1561 tcataattga tgtaactaca ggaaatatta tcaaacaaat gccatttttc ggcgatgggg
     1621 attatgaagg aattgcaagg gtaaataaca gtttatttat attgcggagc gatggattat
     1681 tattcgaaat tcctgatttc cattcacctg aatatgggca tattaccttc aaaacaggca
     1741 tccctgcgaa caacaatgaa ggattatgtt atgatccgga acacaacaga ttgctgattg
     1801 catgcaaaag taatattggt aaaggtgcaa cattaaaaaa tgtgcgcgct atttatgcat
     1861 ttgaccttgg cactaaaaaa cttagcagtg acccagtttt tgaagtttac atttcagaat
     1921 tacaggatta cgctaaaacg catgcaatgg atcttccgga gaaaaagtca aagcacaaaa
     1981 agaaacaaga acaaccggat attaaattta gaccatcagc aattgccatc caccctgaga
     2041 accacaacat ttatttattg tcggcagttg atcatttact ctgtatagta aatcccaatg
     2101 gatcattagt tagtttgatt tcactggatg aaaacgtatt taacaaacct gaaggaattg
     2161 cattttcatc tgataacact atgatcatca ccaacgaagg acaagataaa catccatcct
     2221 tattatcatt cccgtataaa gggagaagat aattaattaa ttaccccgga tttgatctct
     2281 tctacaatat tgggatccag caatgttgtg gtatcgccaa tttgcccggg ttctccttca
     2341 gcgattttgc gcaaaattct acgcataatt ttcccgcttc gcgtttttgg taatccggat
     2401 acgaattgaa ttttatcagg ttttgccaag gcaccaattg ctctggtaac cgtaagtaaa
     2461 atatcttgtc gcgttaattg cggatcagat tgatgaccac ctgtgtacac tacaaaagca
     2521 taaatccctt gtccttttat atcatgcgga tatccaacaa cggcactttc tacaacaccg
     2581 gcatgcatgt ttatagcatt ttctacttca gcagtcccaa ttcggtgacc acttacattt
     2641 aaaacatcat ctacacgacc cgtaatacgg tatcgtccgg cttcatcacg caaggcgcca
     2701 tctcccgtaa aatataattg atgatacgtt gcaaaataat tttgtcggca tcgctcgtga
     2761 tcgccatacg ttgtgcgcaa cattccgggc caggggtgac gaatgcataa atttccgctg
     2821 acattgttac cttcaattat attgccattt tcgtcaacca aaatcgggtc aattccgggc
     2881 aatggataag ttgcatagga tggttttgct tctgtaatac cggctagatt tgaaattaat
     2941 ataccacctg tttctgtttg ccaccaggta tccacaatcg gacagttttc ttttccgata
     3001 tgcttatgat accattgcca cgcttcttca tttatgggtt caccaacagt tcctaaaacg
     3061 cgcagcgaac ttaaatcttt accctcgatt ggcgctgtcc cgaaacccat caaacttctg
     3121 atagccgtag gcgccgtgta taaaatattg actttatatt tctcaacaat atcccaaaac
     3181 ctaccggcat ccggccaggt tggaatacct tcaaacatta aagaagtagc acccgcactt
     3241 aagggcccgt aaacgatata gctatgaccg gttatccaac cgatgtctgc agtacagaaa
     3301 tgtatttctt cgggctgata ctgaaaaaca tttacaaacg aataatttgc ataaaccatg
     3361 tatcctgccg tggtatgcac aacacctttt ggctttcctg ttgatccact ggtatataaa
     3421 ataaacaatg ggtcttcact ctccattact tcaggttcga aattatgaag acctaatgtc
     3481 tccacttttt taatttcatc ttcccaccac acatctctgc ctttcaacat acttaccgga
     3541 gttcttgtcc ttgtgcaaac aatcacacgt tcaacaaaag ggcatgcaat cagcgcatca
     3601 tcaataacgc ttttcaaggg aatatcttta gcccctctga aagcgccatc acaagtaatg
     3661 ataaaatgtg cttgcgcatc ctgtaaacga tcggcaatac tttgggcact gaatccgcca
     3721 aaaattacgg aatggatggc accaactctt gcacatgcca aaactgcaat ggctaattca
     3781 ggaatcatac ccatataaat acagacgcga tcaccctttt caactccgtt atttttcagc
     3841 acatgcgcaa actggcaaac tttatcatgt aattgcctat aggtaagcac acggtgcgac
     3901 tcttcaggat cattgggttc ccaaataata gcaggtttat ttcccagcga ttccaaatgc
     3961 ctatcaatac aattttccgt aatattcaat tgccccccta aaaaccatcg cacatgaggt
     4021 tcaacaaaat tccagtcgag ggttaattcc cattgttttt tccaggtaaa atgcgatgcg
     4081 atatcagccc aaaatgctgc aggattttca atacttttag cataagcatg ttgataggcc
     4141 tcggcagatg ttatttgata tgggacagcc atatttgcga taattaatta tttgaaattc
     4201 agatcatatt ctcaatcctc caatgcattt tcccacttac tgacagcagc tgtagcaatt
     4261 gcatttccga tcacatttgt agcactgcga cccatatcca gaaatgtgtc aatgccgagt
     4321 aataacacca atccggcttg tggtatattg aacattgaaa tagttcccgc aattactaca
     4381 agactggccc gcggaacggc cgcaattccc ttactcgtaa ccatcaatgt cagcaacatg
     4441 gtaatttcct gcgacatagt gagatcgata ttataacatt gtgcaataaa tattgatgcg
     4501 aaggtcatat acatcatact gccatcgagg ttgaaagaat atcctaaggg caaggtaaaa
     4561 ctgacaatct tattattaca gccaaatcgt tctagttcag ttatcaattt aggaaatgcc
     4621 gcttcactgg aggctgttga aaatgccaat aaaaaaggct ccttaattct tcgcaataaa
     4681 atgaaaactc tttttcgtaa tactgcaaat ccggcaataa tgagaatggc ccataatatc
     4741 agcaatccga aataaaaatc gcgcatgaat atgccgtacg ttaagagcac cgatgtaccc
     4801 tttgttgtaa tcacagaaca catggctgca aacactgcaa agggtgcaaa tagcataacg
     4861 taagttgtta ttttaatcat cacgtgtcca actatatcca atgctttaat gagtggcgct
     4921 gctttttccc caattgcagc acaagccact ccgaagaaaa tactgaaaac tactatttgt
     4981 aatatttcat tttcagacat cgctttaaaa atactggttg ggaaaacgtg cgtaataaat
     5041 ttttcaagtg taatagctcc tgcatcaacg cccgcacttg cagtttctgc aggaagtgcc
     5101 aggttcatag cttcccccgg ttgcagcgca ttaaccaaaa acatcccaag cgttagcgaa
     5161 ataatagttg ccgtaataaa ccacaacatt gttttccctc ctacccttcc aacagttttc
     5221 acatccccca ttttagcaat tccaacaatt aaagttgaaa gcaccaatgg cgcaataatc
     5281 attttaatca gtgaaaggaa tacggttgtg gtgatgttga ttttagaagc aatcgatccc
     5341 aattgttcat cagatttccc ggaattatgg atcatggatc cgacaaatat gcccagcacc
     5401 atggctgcca aaatgtagag tgtaagcctg ttagatttct gcatgcacaa gttttgcgcc
     5461 ctaagataat tcatttgacc ttgggtgtaa ggcttgacta ccggttaaat acattttaaa
     5521 cttttcgtaa atgcgcacta taacctgccg accgacacgt aattttgcaa catggccggc
     5581 aaacaaaata aactcttcct atatggcacg ctggtagtag ctgcccttgt agtttttgca
     5641 attttcatgg tttacaataa cactatgagc ggtaataagg agcttgaact gcctttttat
     5701 ggcccacgag gcagcaatgg agaaccacat tatgtgcagg atttctccct gttgaatcag
     5761 gatagtgtat tattggatat gactccctac aaaggaaaaa tctatgttgc cgatttcttt
     5821 tttaccacct gtcagggtat ttgccccatc atgaccgagg gaatgttaaa agtctatcat
     5881 gcctatgaaa ctacacccaa tgtgatgttt ctttctcata ctgtcgaccc ggaccatgat
     5941 acaccctaca ttctgaagcg atatgccgga aacagagata tcaataatgc caaatggcag
     6001 ttcgttaccg gcgataagaa gcagatctac gaccttgcgc gctattcata tcttgttgac
     6061 gcttcagttg gagatggcgg ccctactgat tttgtgcaca ctgagaactt tgcacttgtt
     6121 gaccccgaag gccgtattcg tgggtattat gatggcacag ataccgttga agtcaataag
     6181 cttattaaag acatcagcct cctgatggcc gaataatcta tttttgctga aacccccatc
     6241 cctcaaggat tttgcgcata aaaaatccat gagaaatgct tacctttgca cctccaaatt
     6301 ctgagctgac caatgattac tgttaataat gtatcgcttc gctatggcaa acgccttttg
     6361 tttgaagacg taaacctcaa attcaccaaa ggcaattgct atggtatcat tggtgcaaat
     6421 ggtgccggga aatccacttt ccttaaaatt ctggcaggtg aaatcgagcc taccaccgga
     6481 ttaattgata ttactcccgg cgagcgtatg gcggtattga aacagaacca caacgagttc
     6541 gaccagcact ctgtaattca cacggtgatg atgggtcacc gcaaattatg gaatattata
     6601 caggagaaag acaccttata tgccaagcct gattttaatg aggaagatgg tattcgtgtg
     6661 ggtgaattag aggctgagtt tgctgaaatg aatggctgga atgccgaaag tgatgctgca
     6721 gagctattga gtaacctcgg tgtaaaggaa gatctgcatt atgtgcccat ggaacagatt
     6781 tccggtaaag agaaagtacg cgtgctgatt gctcaggcac tgtttggtaa tccggatatc
     6841 ttgttgatgg atgagcctac caacgacctg gatattgaca ctatttcatg gctggaagac
     6901 tttcttgccg attatgaaaa tacagttatc gttgtttccc acgaccgtca cttcctcgat
     6961 gcagtttgta cgcacgttgc cgatattgat tatggtaaga taaatatctt caccggtaac
     7021 tatagcttct ggtatcaatc atcgcaattg atgttgcaat tgatggctca gaagaataaa
     7081 aaagtggaag acaaacgtcg cgagttacag gaattcatta gtcgattctc tgcgaatgct
     7141 tctaaggcaa aacaagctac ttcacgtaag aaagcccttg aaaagcttaa tgtggaagag
     7201 attcgcgctt cgagcagaaa atatccgggt atatttttca ctcctgaacg cgatcttggc
     7261 gatcaggttt tgcaagttac gaatgtaagc aaatcgcttg agggtcgaaa cctattcagt
     7321 aacgtcaatt ttactgctga aaaaggtgat aaaattgcga tcgtaagtcg ctaccctatt
     7381 gcaaaaactt atttcttcga aattattgcc ggtgaacgcc aactggatca gggtgaattt
     7441 aaatggggtg taacagtaac atctaattac cttccaacag ataacagcag tttctttgcc
     7501 ggtgaaatga acctcatcga ctggttgcgt caatattcaa aagaaaaaga cgaatcatac
     7561 attcgtggtt tccttggtcg tatgttgttc agtggagaag aatcacttaa aagctgcaag
     7621 gtattgtccg gaggcgaaaa agtgcgttgt atgatttcac gcgccatgtt attgggtgga
     7681 aacgcactca tcatggacga accaaccaac caccttgacc tggaaagtat tgaagcactc
     7741 aataatgcat tggctgattt taaaggcttt gtaattttca cttcgcatga ccaccaattc
     7801 atgcaaacga ttgcaaaccg tgttatcgaa atcacgccta atggaataat cgatcgtttg
     7861 atgacctacg acgagtatat tttggatccg aaggtgaaag agcttcagga tcaaatgtat
     7921 ccgtcgagtg tgactgctga atagcctttc aagctactga actaatatac ctgccaatct
     7981 gtttacccag gtaatgcgta aatattggca tgaacagaat acttaattct ttatgtatgc
     8041 gaacacttat tgttgttatt gcattgatgc ctgcaatttt cggatgcagc cagaatactc
     8101 agaaagccac tgaaactaac tcaagtatag aaactttaga taataaggtt acgactgagt
     8161 ctgctgatta tatttttgac ggcaaaatca tcactaagcc tgattcagtg tggcagaaag
     8221 aactcaatgg tatgacgtat tacgtaatgc gtcaggccgg tacggaaaga ccatttcaaa
     8281 atgcctatta cgacaaccat gagaagggca tttattattg cgctgcttgc ggactaccac
     8341 tgttctcatc agaaacgaaa tttgactcag gcaccggttg gcccagttac tatacaccga
     8401 tcaacgactt gaatattggt aaggacaccg atctggatat tggttatgtg cgcacggaag
     8461 tacactgtgc ccgctgtgat ggacatcttg gacacgtatt tgatgatgca ccgcaaacac
     8521 caaccggatt gcgttattgt atcaattctg cctcattggt attcaaaaag caataatttt
     8581 gcgcaacaat cctgcgcaag gtgatcttat acatcattaa aaaagttcct ttattccgcc
     8641 gaatgtattt tcattcgatc ggaaggccat tcgtaggaaa aaaaatcagt gcagttttac
     8701 cctttcttcc taaggatgtt ccggttttag atatcggatc aggcaatgga ttagcagcaa
     8761 gccttatgat ggaggcagga tgcaaaattg ttccgatcga tatacacgaa gggcaatatc
     8821 acgatagtgt caaaccaaca gtctatgatg gcaagcatct gccttatgtc gataatcatt
     8881 tcgaagccgg cgtaatttta actgtcttgc atcatgttga tgatcctgac gctctgctga
     8941 aggaaattat gcgtgtttgt aaatcacttg tcattatgga ggatatctac gaaaatgcgt
     9001 ttcaaaagaa catcactttc tggttagact cacttgcaaa tttgtggtat tctccttgtc
     9061 cacataccaa taaaaatgat ataggctgga aatcactttt taatgcaatg gaccttgaac
     9121 ttacagatgt aaggtatcga aacgcatttt ttgtgataaa gcaagcagta tacgtagtga
     9181 gcaaaaaatc acaatagaaa tttaaatcta ttcgtctctt tgtcgaattg aatattagca
     9241 gaatcgaaat aggttgccgg tatatttctt tcaatcaatg aactcgtttc tccaaactca
     9301 accttaccct ttttgtcgat aaacaataca tgatctgaaa ttgccagagc aagatctata
     9361 tcatgcgagg taaacaaaat tgttttacct gatttagata atttacgcaa aagtgaaaac
     9421 acctcaagtt tattctgcat atcaaggtgg gcggttggct catcaagtat gatgattggt
     9481 gtctcttgag cgagtgccct tgcaatacct accaattgaa attcaccatc gctcaaagta
     9541 tataaactac gatcaattaa atgagaaacg cccgcaactt tagcggattc ttcaattatt
     9601 gcaatatcat gacctgaaac acctgacaac caatgcaaat aaggtgtgcg cgacatcgcc
     9661 agtaattcat aaacgctcag tgatgaaacc atttcccccc tacctgtcaa aacaatactt
     9721 atctgcttcg cgatttcacc tgcactaaag tcagacaagg atttattatg cagtaaaact
     9781 tcaccactca atattttctg caacccacac aagctgcgca aaagtgtcga ttttccggtt
     9841 ccgtttaagc ctacaattga agtgacacta ttctgtggaa cagcaatatt tatatccttg
     9901 aggatgacat gtttatcata accaacacaa agtgctgttg acttaataat atgttgtatc
     9961 aatatcgtca cagcgtacgt ttcaaatgct gatttttata aataatataa attacaaatg
    10021 gcgcacccat aaaggctgta actgcattaa gtgggagcac acctgcatca cctcccactt
    10081 gagtaagcat atcgcaaagc agcattacac acattccaaa caatacacta gccggaatca
    10141 acaggcgatg atcatttacc tttaatacca tccgcgccaa atgcgggatc gcgataccta
    10201 taaatgcaat tggtccgcaa aaagcggtaa ccgtaccggt taaaatgcct gtaataacaa
    10261 ttatgtacaa tcgtaagcgg ggcacatgta tgccaagact caaagcgtat t
//
LOCUS       DAOQOU010000120        10003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3564_length_10003_cov_6.512867, whole genome shotgun sequence.
ACCESSION   DAOQOU010000120 DAOQOU010000000
VERSION     DAOQOU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3564_length_10003_cov_6.512867"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLM90_10410"
     CDS             <1..494
                     /locus_tag="PLM90_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HMZ89831.1"
                     /translation="AAKSMVEEVRRQFREIPGIMEGTGKPDYDRCVAISTEAAIRQMI
                     LPGALALLTPVLVGFGLKGVFDNATSAEILGGLLAGVTVSGVLLAIFQSNAGGAWDNA
                     KKSFEKGVEIDGETHFKGSDAHKASVTGDTVGDPFKDTSGPSMNILIKLMSIVSLIIA
                     AYL"
     gene            672..2363
                     /locus_tag="PLM90_10415"
     CDS             672..2363
                     /locus_tag="PLM90_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ89832.1"
                     /translation="MKKLLLSTFVLISLLTSVQTAFSQATEKYYLRRTPPEPWTWAPI
                     LNTNITEMDNVFGIGGWNSGYYSTVDPDVVFSDNTCFVFMEGGDDHAIDMKNFIDANI
                     TLIESWVTNGGNLFLNAAPNEGVDINCGFGGIVINYPDYASNVNAVDPTHPIFVGPHV
                     PVGTSWSGTYFGHATILGPGLTTLIEDTPSGKAVCSELSWGGGKVIFGGMTVTSWDSP
                     SPQCINVRMNIFEYLSSYVFLDFTYPDSIYCQFEDDPLPVFAVGADTGIFVATPTGLV
                     IDSITGEIDLSASLPGVYEVSNFMDGLSCASGSSTFTVTIAGTPVADAGPDVSLCRGD
                     SVQLDGSGGVTYLWTPPVYLDDPTLEDPTVQAPPTNMFYQLIAYNEFGCPDTDDVMVT
                     LYPDPVIDAGEDQVMVLGGFAALDASGGATYVWTPAEWLDNALISNPTAFPEDTTIFV
                     VIGTDINGCVDTDSVTIFVVEESDILSPTAFTPNGDGLNDTYKPSFVGIGSITEFSIY
                     NRWGSLLYYTADPTLGWDGNFKDNEQEVGTYVVVIKGLTQQGDPIVKTSTLALLR"
     gene            complement(3070..6021)
                     /locus_tag="PLM90_10420"
     CDS             complement(3070..6021)
                     /locus_tag="PLM90_10420"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89833.1"
                     /translation="MKKLFTLLAAFSCLLGMKAQDAHQKDPFAKTFIQNTQRVPNQQL
                     QATFRDAKAWENFRAAHGNWWVEFKEETGTPHRAYGQPIDVAGATPEDKAINFFETYC
                     KDYIPTTQGLVLTSSNTTDKYHYVSFIQQYNGLDVLWSEATVRINMQGEVAMFGLDFY
                     KGIGISTTPAIDAASIGAFATTGIDVPVLGTEVAPALKVLPIPGATGMEYKLVYEVTV
                     HTANVNDIPGAYYTLVDANNGFVYYRANRVHACGEYLMGANIQINADITDNPLLATVN
                     RGLPYLRVKVDGVNYYTDANGFLSLNTITAPTTARIYLRGLYARVSQGTGATNIDSAD
                     ITVFPGDNVIQFDDEAGTVPSEVSAYYWQNIVHDHMKSYFPSFTDLDVDQLIRVERND
                     GSCNAFYDGTSTNFYQNGGGCPSTGLFNDVVMHEYGHGVNYDLYAALGDPGGMGNGAM
                     QEGYADLWGITVTDNPVMGQGFLGGAGTFVRRYDAEPKVYPQDLVGEVHADGEIIAGA
                     WWDVSENLGGDIESMTALWMETHNATVDGPSGSEGTIYTDVLIEALFADDDNGDLTDG
                     TPNYNAIVEAFCEHGITMIGQVSTDHIGTFDFINSDEPISINVTIDFDYPTYVSDVKM
                     YYKVNNDPSWTESVMTSVDAVNYVGEIAPQIAGSVISYYIQFSDINNCGGTVLPAEAD
                     LVDPNLPYFALVGAYQWHVEDFDNVFGSWEVDPFGTDDASTGEWDINTPIPSTDDVGY
                     TVQTGEDHTVGDGNLCAFTGNAGEFDGVGTNDVDGGKTTLRSPFIDLTIYNDPIIVYY
                     RKYSNDSPTSANPGNDVWQVYITDNGGDSWMKLERTYTSDNTWRRNAFRVKDYVDVTE
                     DFGMIFIAQDSIDPSDPSGFLGGSLVEAAIDDMEIWDTRMPEVHVNDIETNSITVYPQ
                     PTNSYLNIVSDILISQVCLIDISGKIVSAESFDNLHLVSLEVGQIPKGIYSLKLNFCN
                     STTGYKTVIIN"
     gene            complement(6175..6939)
                     /locus_tag="PLM90_10425"
     CDS             complement(6175..6939)
                     /locus_tag="PLM90_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ89834.1"
                     /translation="MIVTILGSGTSGGIPMIGCPCPVCRSTDSRDKRLRTSIHISING
                     KSFVIDAGPDFRQQMLRESISRLDAIIFTHQHRDHTAGLDDIRAYNYFQAKPMDIYLS
                     SEVEASLKKEYHYVFEGGGYPGLPEMRLFRIENRAFYIDEVKITPIEVMHYKLPVFGF
                     RVNNFTYITDANAISEQEIEKIKGSEVLIINALRKERHISHFSLEEALDMIRKINPQK
                     AYLIHMSHQMGAHAEVSMELPENVELAYDGLRIECA"
     gene            complement(6936..7208)
                     /locus_tag="PLM90_10430"
     CDS             complement(6936..7208)
                     /locus_tag="PLM90_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89835.1"
                     /translation="MFKYTTNTLKKIEDLLKEAGYIVRYERGNFMAGYCILESKKVVV
                     INRYYETESRINSLLEIMGKINLEGEELSESSRDFLHEIQLQTVKV"
     gene            7288..7908
                     /locus_tag="PLM90_10435"
     CDS             7288..7908
                     /locus_tag="PLM90_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ89836.1"
                     /translation="MRIILFTTILFTFMVADAQINAGDPKDNEFHLFDAGFSVGANFS
                     QVDGDYYAGFNKLGITAGPIIHLNFNPNWSASLELLYSQKGAKSKPDPNNVNTYLFVL
                     DYAEVPVLINYNDKNRLLFQAGLAYGRNIKAFEEVNGIDTNGGEVIKSDELSYVLGGT
                     FLIGEMKHWGANFRYEGSITKVGPSPNPKVVGLVNRLIAVRGIYYF"
     gene            complement(8166..9494)
                     /locus_tag="PLM90_10440"
     CDS             complement(8166..9494)
                     /locus_tag="PLM90_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89837.1"
                     /translation="MKKLISTGLAASLLFAGTLHAQRNCDAPNSGLTPFLDLQTGTYM
                     GYQAGLYPGGTNTLTGPHLKSGKTIAKGIKPLDGDGNVNFGDGVVLVAGFGPSVPGHI
                     YNKFVEHVRTPSEKYDMNPCMDAINLCVGGKDISYPTSDSLYVAYWEGLVQNVYDVGY
                     TPQQVQIGWMYFNAKGLTEPPAFPGKSLEMEELYIQFINKAKEYFPNLKIMYMSSRHW
                     GGFADTTITEYYSLAEPASYQNSWSVKWTVEAQINGTEPRLQYKGAAPKAPYILWGPN
                     FWCDGELKRMWDDKKYICELSFDEDDGYHLSDQQDSKDALDILDVMYFGEVGKQFTKN
                     SATWEFCVPWLDTLAELHGYREDPNKTLGIFKVSPNPGVNEFYISLPTEKYAAQITIV
                     NELGQVVDAFLHEENFSTEVLIDMTGRPSGMYFAKVMMNGEVHTLRFMKQ"
     gene            complement(9598..>10003)
                     /locus_tag="PLM90_10445"
     CDS             complement(9598..>10003)
                     /locus_tag="PLM90_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HMZ89838.1"
                     /translation="AYEIARRSRGTPRIANNLLKRIRDFAQVKGGGTIDVEIARFGLK
                     ALNVDEHGLDEMDNRILNDIIEKFAGGPVGLTTIATAVGEDAGTIEEVYEPFLIQEGY
                     IKRTPRGREATEKAYRHIGKVPPGGAYRLFDE"
BASE COUNT         2807 a         2050 c         2095 g         3051 t
ORIGIN      
        1 aagctgcaaa aagcatggtt gaagaggtgc gtcgtcagtt ccgcgaaatt ccgggaatca
       61 tggaaggaac aggcaagcct gattacgatc gttgtgtggc catttctact gaggctgcta
      121 ttcgccagat gatattaccg ggtgcattag cattattgac accggtgctc gtaggtttcg
      181 ggcttaaagg tgtatttgac aatgccacct ccgctgaaat tcttggtggt ttgcttgcag
      241 gtgtaaccgt gagcggtgtg ctattggcaa tcttccaaag taatgccggt ggcgcatggg
      301 ataatgcaaa gaaatcattt gaaaaaggtg ttgaaattga cggagagaca cactttaaag
      361 gttctgatgc acacaaggcg tctgttacag gcgatacagt tggagatcca tttaaagata
      421 cttcaggtcc aagtatgaac attctcatca aactgatgag tattgtcagc ctgatcatag
      481 ccgcatattt ataattacag tcgaatcata ttttgaaagg catcccaatg tggatgcctt
      541 tttttcgtct gaaaaggctc atttttttat gaattaaagc gtttttaaca ttcaaagtgc
      601 caaaaaaccg aaattcacat taactttaga gtggaatact atgttataac ataccgatgc
      661 aattggcaac tatgaaaaaa ctactcctct caacattcgt tttgattagc ctgctgactt
      721 ctgttcaaac agcttttagt caggctacgg aaaaatatta tctgcgcaga actccccctg
      781 aaccgtggac ctgggcacca atcctgaata ccaacattac cgaaatggac aacgtgtttg
      841 gtattggtgg gtggaattcg ggctactact ctacagtgga tccagatgtg gtgttctctg
      901 acaatacctg ctttgtattt atggaaggtg gcgatgacca cgccattgat atgaagaatt
      961 ttattgatgc caacataacg ctgattgaaa gctgggttac taatggcgga aacctgtttc
     1021 tgaatgcggc acctaacgaa ggtgtagata ttaactgcgg ctttggaggc attgtaatta
     1081 attatcccga ttacgccagt aacgtaaatg ccgttgaccc cacgcacccg atatttgtag
     1141 gtcctcacgt acctgttgga actagttggt caggaacgta ttttggtcac gctaccattt
     1201 taggtccggg attaacaaca ttgatagaag atacaccttc aggtaaagcc gtttgttctg
     1261 aattatcttg gggcggtggt aaggttattt tcggtggtat gactgttaca tcatgggatt
     1321 caccatcacc gcaatgtatt aatgtgcgca tgaatatttt tgaatatctc agctcatacg
     1381 ttttccttga ttttacatac cctgattcca tctattgtca gtttgaggat gatccactgc
     1441 cggttttcgc agttggtgct gatacaggaa tttttgttgc cacgccaacc ggacttgtga
     1501 ttgacagcat tacaggtgaa atagatctga gtgcttcatt acccggagtt tatgaagtat
     1561 caaacttcat ggatggttta tcttgtgcga gtggttcatc tacatttacg gtaaccatag
     1621 caggaacgcc agttgctgat gcaggacctg atgtttcatt atgtcgtggc gattctgttc
     1681 aattggatgg tagtggtggc gttacttatt tgtggacacc tccggtttac ctcgatgatc
     1741 cgaccctgga agatccaacg gtgcaggcac cacctaccaa tatgttttat caactcattg
     1801 cctataatga attcggttgc cctgacacag atgatgtgat ggtaacgttg tatccggatc
     1861 cggttattga tgcgggtgaa gatcaggtaa tggtgttagg tggatttgca gcattggatg
     1921 cgtcaggtgg tgcaacatat gtttggacgc ctgcagaatg gttagacaat gcattgatat
     1981 ctaatccaac cgcattccct gaggatacaa caatttttgt agttatcgga actgatatta
     2041 atggttgtgt tgataccgat tcagttacca tttttgtggt tgaagaatca gatattcttt
     2101 caccaactgc attcacacct aacggcgatg gattaaatga tacctacaag ccatcttttg
     2161 ttggtatcgg atctattaca gaattcagta tctacaatcg ctggggaagc ttgttgtatt
     2221 atactgctga tcctacatta ggttgggatg gcaatttcaa ggataatgaa caagaagttg
     2281 gtacttatgt agtagtgatt aaaggtctga cgcagcaagg tgatccgata gttaagacga
     2341 gtacgttggc gctgttgaga tagcgatcaa tgtttatcat attctaaaaa gccgatgtcg
     2401 atttgatatc ggcttttttc gtgaggcgtg aggcgtgact cgtgagtcgt gaagcgtgaa
     2461 tcgtgagtcg tacgttgtga gcttgccaat cctttggcgg atgaggcttt gcgactttgc
     2521 ccctttgcga ctttgcgcga aaaaagggat tcaatttgaa gttgaggatg actatgattg
     2581 gtaaaatcta ttcattaatt ccattataaa aatccattat tgatattcaa cttaacttaa
     2641 ttcttagcta catacaatta ctaatataac tcgattagct tttatagaaa cagaaaagta
     2701 tttctgaatt agagacatca gacaaaaacg ccgtccagaa tctgaacggc gttttgtaat
     2761 tattttatta gatcatcagt gttgtatgat gattcgatta gttcagtcaa tattgatgta
     2821 tactcaatat ccggaattct tgtagaaagt ttcacaaaca ttgaactaga aaatactcaa
     2881 ataaatttta atttatcgaa tctatctagt ggtgagtatt taataaattt aactggtgaa
     2941 caatttcaat cttcaagact aataacaatt caaaaatagg gacaaaatcc ttaaatttat
     3001 tgttctcaat ttcggttttc aacaaataat aaaaacgaca ggtcttgcct gtcgttttta
     3061 ttattgaaat taatttataa ttactgtttt gtaaccagtc gtagagttac aaaaattcaa
     3121 ttttaatgag taaatcccct taggaatttg tcccacttct agactaacga gatgtaaatt
     3181 atcaaaactt tcagcagaaa caattttgcc actaatatca attaaacaaa cctgagaaat
     3241 gagaatgtcc gaaactatgt tcaaatatga atttgtcggt tgtggataaa cagtgattga
     3301 gtttgtttcg atatcattta catgtacttc tggcattcgt gtgtcccaga tttccatgtc
     3361 atcaattgca gcttccacta agcttccgcc taaaaagcca ctaggatctg atggatctat
     3421 actgtcctgt gcaatgaaaa tcattccaaa atcttcagtt acatctacat aatccttcac
     3481 tctaaaagca tttcttctcc acgtattatc tgaagtataa gttctctcaa gtttcatcca
     3541 tgaatcacct ccgttatcag ttatatatac ttgccaaaca tcattaccgg gattggcact
     3601 tgtaggcgaa tcattagaat attttcgata gtaaactata attgggtcat tgtaaatagt
     3661 caaatcaata aatgggcttc tcaatgttgt cttgccgcca tctacatcat ttgtacccac
     3721 accatcaaac tcacctgcat tgccagtaaa agcacaaagg tttccgtcac ccaccgtatg
     3781 atcttcacct gtttgaactg tataacccac atcgtcggta gaaggaattg gggtatttat
     3841 atcccattca cctgtgctag catcatctgt tccaaatggg tctacttccc agcttccaaa
     3901 aacattatca aaatcttcta catgccattg atatgcgccc acaagtgcaa aataaggtaa
     3961 attaggatca accaaatctg cttctgcagg caaaactgta ccaccacaat tattaatatc
     4021 agaaaattga atgtaatatg aaataactga acctgctatt tgaggtgcaa tctcgccaac
     4081 gtaatttaca gcatcaaccg aagtcataac agattcagtc caagaaggat cattgttgac
     4141 tttataatac atcttgacat cgctgacata ggttggataa tcaaaatcaa tggtaacatt
     4201 tattgaaatt ggttcatctg aatttatgaa atcaaatgtt ccaatatggt ctgtcgaaac
     4261 ttgtccaatc atcgttattc catgctcaca aaacgcttca actattgcat tataattagg
     4321 ggttccatct gtcaaatcac cgttatcatc atctgcaaat aatgcctcaa tcaaaacatc
     4381 tgtatatatt gtaccttcag aaccacttgg cccatctact gtagcattat gtgtttccat
     4441 ccatagtgca gtcattgatt ctatatcccc tcctaagttt tcagaaacat cccaccacgc
     4501 acctgcaata atttcgccat cggcatgcac ctcgcccacg aggtcttgcg gataaacttt
     4561 gggttcagca tcataacgac gaacaaatgt tcctgcacca ccgaggaaac cttgtcccat
     4621 tacagggtta tccgtaacag ttattcccca caaatcggca taaccttcct gcatagcgcc
     4681 gttgcccata ccacccgggt cgccaagtgc agcatataaa tcataattaa cgccatggcc
     4741 atattcgtgc atcacaacgt cattaaataa acctgttgaa gggcagccgc caccattttg
     4801 atagaagtta gttgatgttc catcataaaa ggcattacag gatccatcgt tgcgttcgac
     4861 acgtatcaac tgatcaacat caagatcagt aaatgatggg aaataagact tcatatgatc
     4921 gtgcacaata ttttgccagt agtatgcgga aacttccgat ggcactgtcc cggcttcatc
     4981 atcaaattga ataacattat cgcccggaaa cacagtgata tcggcactat caatattagt
     5041 tgcaccggtt ccttgactga cccgagcata taaaccacgc aaataaattc tagcagtagt
     5101 aggtgctgta attgtgttta gtgagagaaa cccgttggca tccgtataat aattaacacc
     5161 atcaaccttt actctaaggt atggcaatcc gcggttaact gtagctaaca gtggattatc
     5221 agtgatatca gcattgattt gaatattagc acccattaaa tattcaccgc atgcatgtac
     5281 acgattcgca cgatagtaaa cgaaaccgtt atttgcatct accaatgtgt aatatgctcc
     5341 gggaatgtca tttacgtttg cagtatgcac tgttacttcg taaacaagtt tgtattccat
     5401 gcctgtagca ccgggaattg gtaacacctt caatgcaggt gctacttctg ttccaagaac
     5461 aggaacatcg atacctgttg tagcaaatgc accaatactc gctgcatcaa ttgcaggagt
     5521 tgtagagatg ccaatgcctt tataaaaatc aagaccaaac atagcaacct caccttgcat
     5581 attgatacgc acggttgctt cagaccacaa tacatcaagt ccgttgtatt gttgaatgaa
     5641 gcttacatag tggtacttgt cagttgtgtt gcttgaagta agtactaaac cctgagtagt
     5701 agggatgtaa tctttgcaat aagtctcgaa gaaattaatt gccttgtctt caggagttgc
     5761 acctgcaaca tcaattggtt gtccataagc acggtgtgga gtaccggttt cttctttgaa
     5821 ttcaacccac caattgccat gcgcggcacg gaaattttcc catgctttag catcgcggaa
     5881 agtggcttgt agttgctgat ttggaactct ttgcgtgttt tggatgaatg ttttggcgaa
     5941 aggatctttt tgatgagcat cctgtgcctt catccccaac aagcaagaaa atgctgcgag
     6001 gagcgtaaac aattttttca taattggtgt gaagatttga ttactgtttg atgttaatgc
     6061 ctgattgagt tgtggttgtt ccaggatcaa acggatatca aagataataa agcgttttta
     6121 gctacacaac cgggcatttg tcagagtaag gtcataagcg caggtggcaa tctgttatgc
     6181 acattcaatt ctcaggccgt cgtaagcaag ttcaacgttt tccggcagtt ccatgcttac
     6241 ttcggcatgc gcgcccattt gatggctcat gtgaatgagg tatgcttttt gtggatttat
     6301 cttacggatc atgtccaagg cttcttccaa ggagaaatgg gaaatatgtc tttcttttcg
     6361 taaagcattg ataatcaata cttcagagcc tttaattttc tcaatttcct gctcggaaat
     6421 ggcatttgcg tcagtgatat aagtgaaatt attcacccga aagccaaaaa ccggcaattt
     6481 gtaatgcatg acctctatgg gtgtgatttt gacttcgtcg atgtaaaaag ctcgattttc
     6541 aatcctgaaa aggcgcattt caggtaaacc cgggtatccg cctccctcaa aaacatagtg
     6601 atattctttt ttcaacgaag cctccacctc actgctgagg tagatatcca ttggttttgc
     6661 ctggaagtaa ttataggcgc ggatatcatc gagtcctgcg gtatgatcgc ggtgctgatg
     6721 ggtaaaaatg attgcatcga ggcgcgatat ggactcccgg agcatttgct ggcggaaatc
     6781 gggacctgcg tcgatgacga acgattttcc gttgatactg atatggatgg aagtgcgaag
     6841 gcgtttatct ctagaatcgg tgctgcgaca taccggacaa gggcatccta tcatcggtat
     6901 accaccggaa gttcccgagc cgagtatggt tacgatcaca cttttactgt ttgcagctgt
     6961 atctcatgaa gaaaatcgcg cgacgattcg ctgagctcct ccccttccag attgatctta
     7021 cccatgatct ctagcaggct gttgatgcgg gattcggtct cataatacct gttgattacc
     7081 accactttct tgctttcgag gatacaatag ccggccataa agttgccgcg ttcgtagcga
     7141 acgatgtatc cggcttcctt taacaggtct tcgatctttt ttagggtgtt ggttgtatat
     7201 ttgaacatca cgaatgtcgt tatttacttt gtgctgccaa aaataatggc agtgccgctg
     7261 tattgaaaac gaagtaatgc acatcaaatg aggataattc tttttaccac catcctattc
     7321 acattcatgg ttgccgatgc acagatcaat gccggtgacc cgaaggacaa tgagttccac
     7381 ttgttcgacg ccggttttag tgtcggagct aatttttcac aagtcgacgg cgactattat
     7441 gccggcttca ataaacttgg tattacagcc ggaccaatca ttcacctcaa tttcaatcct
     7501 aattggagtg cgagcctcga actgctttat tcacaaaaag gtgctaagtc aaaacctgat
     7561 ccaaataacg ttaatactta cctattcgtt ctcgattatg cagaagttcc tgtattgatt
     7621 aattataacg ataagaacag attgttattt caggcaggct tagcctatgg acgaaatatc
     7681 aaggcctttg aagaggtgaa tggcattgat acaaatgggg gcgaagtcat taaatctgat
     7741 gaattgagtt atgttctcgg tggcaccttt ttaattggtg aaatgaaaca ctggggtgct
     7801 aatttcagat atgaaggatc tattaccaaa gtaggtccat cacccaatcc aaaggtggtt
     7861 ggtttggtga ataggttgat agcggtgagg gggatttatt atttctagga cgtgagtggt
     7921 gagtggtgag tggtgagtgc tgaatggtga gtggtgagta gtgagtggtg aataatactt
     7981 gatacctgat acctgatact tgatacctga tacttgatac ctgatacctg atacctgata
     8041 cttgatacca attctagatt ctaatacgag taatccaaac aatcgcagat tcaaattgag
     8101 atgaaaagca aaacgccgca tggaaaatat ccatgcggcg ttttgatatg ataagggatt
     8161 ttgaattatt gcttcataaa tcgcaatgta tgcacttcac cattcatcat tactttggcg
     8221 aaatacatac ctgaagggcg tcctgtcata tcaatcaaca cttcggttga gaaattctct
     8281 tcgtggagga aggcatcaac tacttgtccg agttcgttca cgatggtgat ctgagcggca
     8341 tatttttctg taggcagact gatataaaat tcatttactc cggggttagg agatactttg
     8401 aaaataccca gtgttttatt cgggtcttcg cggtaaccat gtaattcagc cagagtatct
     8461 aaccaaggca cgcagaattc ccatgttgca ctattttttg tgaattgttt tccaacttcg
     8521 ccaaaataca tcacatcgag gatatccaat gcatctttgg agtcttgttg atcgctgagg
     8581 tgatatccat catcctcgtc gaatgataat tcgcagatgt attttttatc atcccacatg
     8641 cgcttcaatt cgccatcgca ccagaagttt ggtccccata aaatgtatgg tgcttttggc
     8701 gctgcgcctt tatattgaag gcgtggttct gttccattga tttgtgcttc aacagtccat
     8761 tttaccgacc agctgttttg atacgatgcc ggctccgcaa gtgaataata ttcagtgata
     8821 gtagtatcag caaatccacc ccagtggcgt gaactcatat acatgatctt caggtttgga
     8881 aaatattctt ttgccttatt gatgaactga atataaagtt cttccatttc caaagatttg
     8941 cccggaaatg ccggtggttc tgtaagacct ttcgcattga aatacatcca gcctatctgt
     9001 acttgttgtg gtgtatatcc aacatcataa acgttttgta cgagtccttc ccaataagca
     9061 acataaaggc tatctgatgt cgggtagctg atatcttttc ctccaacaca aaggttgata
     9121 gcatccatac aaggattcat atcatacttt tcagaaggag tacgaacgtg ctctacaaat
     9181 ttgttgtaaa tatgtcccgg aactgatggg ccgaaacctg caaccaaaac tactccgtct
     9241 ccgaagttaa cattgccgtc gccatcaagt ggttttatgc ctttggcgat tgttttgcca
     9301 cttttcaggt gaggccctgt aagggtattg gtaccacccg gatataatcc tgcctggtag
     9361 cccatgtagg tgcctgtttg taaatcaaga aagggagtca accccgagtt aggggcatcg
     9421 caattgcgct gtgcgtgtaa tgttccggcg aacagcaggg aggctgccaa accggtggag
     9481 attaattttt tcaaggctta atagtttaag ttgccgttaa attgatgtgc aaagataaca
     9541 aatgtcgggt gttaaaaccg gatgtaatgt tgacgatact tacttgtaaa aggttggcta
     9601 ttcgtcgaac aatctgtagg cgccacccgg aggaacctta cctatgtgtc tataggcctt
     9661 ttctgttgcc tcccttcccc gaggagttct tttaatgtat ccttcttgaa tcaaaaaggg
     9721 ttcatacacc tcttcaatgg tgccggcatc ttctccaacg gcagtagcaa tggtggtaag
     9781 gccaaccggt ccaccggcaa atttctcaat aatatcgttc aggatacggt tatccatttc
     9841 gtcgaggcca tgttcatcca cattaagggc tttgagtccg aaccgagcta tttcaacatc
     9901 tatggttcca ccgcctttta cctgggcgaa gtcacggata cgtttaagga ggttattggc
     9961 tatcctaggg gtaccgcgac ttctgcgggc tatttcatag gct
//
LOCUS       DAOQOU010000121        10002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3566_length_10002_cov_5.309842, whole genome shotgun sequence.
ACCESSION   DAOQOU010000121 DAOQOU010000000
VERSION     DAOQOU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3566_length_10002_cov_5.309842"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PLM90_10450"
     CDS             complement(<1..1272)
                     /locus_tag="PLM90_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HMZ89839.1"
                     /translation="MYAIVDIETTGTNAGHHGITEVAIYIHDGNKVTDHWSSLINPKS
                     EIPLFIQQMTGITNEMVATAPTFEEVAAEIYHLLHPHIFVAHNAGFDYGFIKAQLAEN
                     GYDLHAKKLCTVRLSRKIIPGMRSYSLGRLCESLGIVIHDRHRAAGDALATVHLFQQL
                     LEKDHGGHIPAALKRNTKEQHLPPNMERKMLDALPSGCGVYYFHDAAGKIIYVGKAIN
                     IRSRVFGHFTYAEHDGKENALRDAVFDISYELTGNELIALLLESEEIKRKFPRFNNAQ
                     KRWDRNFCIFTFTDQKGYRHLAIERFSPKKEVLKVFPDFLSARGYLNEILQTFELCGK
                     MCHLQTVKGACYNHEIGLCSGACIGLESPGDYNLRVEAAISNFSEEDHTFFIFGKGRQ
                     DGEQSVICVEKGHYLGFGFFDPENAPQDPEFL"
     gene            1489..2235
                     /locus_tag="PLM90_10455"
     CDS             1489..2235
                     /locus_tag="PLM90_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ89840.1"
                     /translation="MGMIIRFLKGLYTMYTAIIFILLLLITFPFFMVLSLWKDKALLP
                     MLWLCRFIALGLMIFCGIIYRFHDRNKYERNKAYVLIANHRSNLDAPVAAISGKGRVR
                     YLAKKELLKVPVMGQIFAVMSVYVDRSSPQDRRKSVERLKECVKSGDSIFLFPEGTRN
                     KTDQPMIDFKDGAFTIAVQTQTPILPMLYINTDTLMPNSKPLMRPGIIDVYYLPPIEV
                     AGLTDADIPALKQRTRDMLMLRYLELSGDK"
     gene            2284..3678
                     /locus_tag="PLM90_10460"
     CDS             2284..3678
                     /locus_tag="PLM90_10460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ89841.1"
                     /translation="MQLQFCGAAGTVTGSAHLLTLDDGTKILLDCGLYQGNEPEYANF
                     NERFLFRPDEIDIVVLSHAHIDHCGRLPKLVKDGFRGRIYATHATRDLTAILLLDSAH
                     IQDRESSFTNRRRKDDHEDEPLYDAKDVADTMELFRTVGYNKWFGIHPDVEVVYKDAG
                     HILGSATVTLRIKRHGMRDYLLGFTADIGRPDRPILRDPVPMDACDFLICESTYGDRL
                     HEEAPQEKEHFLRVIQQTCVTQKGKLIIPAFSVGRTQEIVYMLDQLVRENRLPDVPVY
                     VDSPLAVNATRVFEMHPECFDKEILDYMALDPNPFGFEKLKYTRSVEESKAINNKKGC
                     IVISASGMINAGRVKHHVFNAIEHPENTILIVGYCADNTPGGQLRNGAKQIRMFGEYK
                     SVNAHVEIMRSFSAHGDYREMIDFLRSQEKDKLRNIMLVHGDKNALTGFRDHLHEAGF
                     GQVDIPKLGDKVDL"
     gene            complement(3698..4198)
                     /locus_tag="PLM90_10465"
     CDS             complement(3698..4198)
                     /locus_tag="PLM90_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89842.1"
                     /translation="MKHLLSFFAISLFVISTHAQDWEVPRHYMLEGAEDYVPYEADII
                     QAANWLRDVSPTTDVEKFKEASQFVIKWVNGSPTVTVEINKTIMEFNEKNPNMLVLYM
                     ASCAKYVLENNYSKDVQAKHISALKDMAIAYQKQPQMQKDKKMEKLVKAIEEGTADDW
                     YNKNFK"
     gene            complement(4273..4815)
                     /locus_tag="PLM90_10470"
     CDS             complement(4273..4815)
                     /locus_tag="PLM90_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89843.1"
                     /translation="MKKITYLFILLPFLISINSCKNEETPDPVNEEELITSVVLSCEN
                     NADLSTSTFAFRDIDGPGGDAPSEFDTIRLNNGYYHLTVSILNESVSSALDLTAEIDE
                     ESTDHQLFYTVDGADITFDYNDTDANGLPIGLDMNVAVGPTSSTGTVTVVLKHQPGIK
                     DGSISTGESDVEVTFVTETN"
     gene            complement(4842..7169)
                     /locus_tag="PLM90_10475"
     CDS             complement(4842..7169)
                     /locus_tag="PLM90_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ89844.1"
                     /translation="MKHVLFVCVMMSALTQLKAQSDNCNLRFSGRAVDMDHKEPLSYA
                     TLYINETQTAVVADENGKFMFEGLCPDTFTLVISHIGCTTSTFTIALTEDTFKEFRLD
                     HTSENLESIVVHGEQDKNTGTLTETTLSGKKLDRLSGKSLGEALKDVNGVSSLQTGNG
                     ISKPVIHGLHSNRILIMNNGVRQEGQQWGNEHGPEIDPFIANSLTVIKGAASVQYGSD
                     ALGGVILVQPAPMPTQKGVSGDLHAVGFSNTRGGAASLMLQYKASDSAALSYRLQGSI
                     KREGNVKTPNYFLANSALAEQSGAATIDYHRNNLHSQVYYSYFHNKVGIFSGSHIGNL
                     TDLQTAFASDTPLVHQDFTYIIGRPYQDIVHHLIKNETNFHHERLGTFTLITAWQNDL
                     RSEYDAHTSLIDSIAALGLPSLYFEIGTITADLKWEQHPGKRHNAVIGISGMNQQNIT
                     RYSIFIPNFKNYSGGIYAIENYKFGRVLLEGGIRYDYKWMQIFRYEGNTLETPTHQYG
                     NLSGIAGMQFFDGRHTQYNLNFSSAWRSPSVSELYSDGLHHGSASLEYGNDSLVSERA
                     YQIIAGIEHQSDKWYFDIGVYNNFIYNFIYLEPTLPAELTIRGAFPVFHYMQTNANLS
                     GADVQTSYMIIPQLKAEIKASTLRARDVANNKWLVMMPADKISFDLEYQPADFKWMHD
                     NAIHAGVEYVFEQKRAPLDEDYVPPPSAYTLFSAGIEGVIALHANEISWSLSADNLLN
                     TAYRNYMNRFRYFADDMGRNITLRIKIPITGKSNL"
     gene            complement(7217..7573)
                     /locus_tag="PLM90_10480"
     CDS             complement(7217..7573)
                     /locus_tag="PLM90_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89845.1"
                     /translation="MMQFLRRSSALSLIATILLLGALSGKDLVHFTFAHDVSHQYGSD
                     SRECCKQDHRQPHIHELNYCFLCHLEVSVTLHEERVVLIPGSFSYDLIPVAHLNSVHQ
                     PGFQGYSDLRGPPFTV"
     gene            7740..9782
                     /locus_tag="PLM90_10485"
     CDS             7740..9782
                     /locus_tag="PLM90_10485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HMZ89846.1"
                     /translation="MRKYPLWLLAALMVSVSACKSTKAPEEDAYQAFDPASLDSTVRP
                     GDDFYAFVNNKWIAAHPIPADKAKYGAFYMLDDNSLTTLRSAMETAAKANAAKGTNTQ
                     KVGDFWTSGMDTNAIENAGYTPIKPWLDKINAIGNADELMRTVAQMHRTYTFAMFTNW
                     VGQDDKNSADVIMNMWQGGLGLPDRDYYTRTDAKSQQIRADYLVHLKNVFMLIGADDA
                     TASKNANTVMSIETALAKASMTNVEMRDPNKIYHKMPLADFQKLTPNMNWNVYYTELK
                     TPEFKTGLNVAQPDFYKALNGMMKSVSLNDWKTYLTYHFVDGASDYLSAPFVNEHFDF
                     HGKKLNGIEELKPRWKRVVETADFCLGEALGEEYVKVAFSPESKERMLEMVGNIKTAL
                     GQRIQNLEWMTDSTKQQALHKLSTFTVKIGYPDKWRDYSNLTITRDAYVLNVMAAAEF
                     EAQRNYNKIGQPVDKSEWGMTPQTVNAYYNPSNNEIVFPAAILQPPFFDPKADDALNY
                     GGIGAVIGHEITHGFDDQGRMYDANGNLNIWWTDKDNSQFVERANVVVTQFNGYCPLD
                     SLCINGELTLGENIADFGGITTAYQAYTMTEEFKAGKSIDGFTPQQRFFLGFGRIWAG
                     DYREDAMRTQLLTNVHSPGEWRVKGTLVNIPEWYEAFGVKEGDKMYLAPDQRAKIW"
BASE COUNT         2884 a         1938 c         2203 g         2977 t
ORIGIN      
        1 gaggaattca gggtcctgag gggcgttttc agggtcgaaa aagccaaatc ctaagtaatg
       61 ccccttctcc acacaaatta cactctgctc tccatcttga cgccctttgc cgaaaatgaa
      121 gaaagtatgg tcttcttcac tgaaattgga aattgcggct tccactctaa ggttgtaatc
      181 gccgggactt tccaatccga tacaggcacc tgaacaaagg cctatttcgt gattgtaaca
      241 agctcctttg actgtttgga ggtgacacat tttaccgcat aattcaaaag tctgcaatat
      301 ttcattcaag tagcctctcg cactcaggaa atcgggaaat actttcaata cttccttttt
      361 aggggaaaat cgttcaatag caagatgtcg atatcccttt tgatcggtaa aggtgaaaat
      421 acagaaattg cgatcccatc ttttttgtgc attgttaaat cgcgggaatt tgcgttttat
      481 ctcttcactt tctaataaca gtgctataag ttcgttgcct gtaagttcat aactgatgtc
      541 aaaaacggca tcgcgaagcg cattctcctt tccatcgtgt tcggcataag taaaatgtcc
      601 gaatacgcga cttcgaatat taatggcctt tcccacataa attattttac cggcggcatc
      661 atgaaaataa tatacaccgc aacctgatgg caaggcgtcc aacatttttc tttccatgtt
      721 ggggggaagg tgctgttctt tggtattgcg tttcagtgct gccggaatgt gacctccatg
      781 atccttttcc aggagttgtt gaaataaatg cactgttgct aaagcgtcgc ctgcagctcg
      841 atgtctatcg tgaatgacga taccaagcga ttcgcacagt cgccccagag aataacttcg
      901 cattccggga ataatcttgc gactaagacg aacggtgcat agtttttttg catggagatc
      961 gtaaccattt tcagccagtt gcgcttttat aaaaccatag tcgaagccgg cgttatgtgc
     1021 tacgaaaata tgcggatgaa ggagatgata aatttctgcg gctacctctt caaaagttgg
     1081 tgcagtagct accatttcat tggtaatacc tgtcatctgc tgaataaaca gagggatctc
     1141 cgatttggga ttgatgagtg aactccaatg atcagttact ttattgccgt catgaatata
     1201 aatcgccacc tccgtaatgc catgatgtcc ggcattggtt ccggtggttt ctatatcgac
     1261 aatggcatac attgcctaaa ggtaagatgc tgtctgcaga aaatgcgata agatgcaaac
     1321 gctgtgatgt gtaaatttgt ttttcaggat attcacttgg cgtatcggca taccttgaat
     1381 cagcctgaat tgagtgatgt cagcatttgt ttttgagtgg aattgctttc ctcttgtcag
     1441 caggtttgaa ggtgcttata cctccccaat aatcattacc ttagtcccat gggaatgatc
     1501 attcgattct tgaaggggtt gtataccatg tatacggcaa taattttcat cttactgttg
     1561 ctgatcacat ttccattttt catggtgctt tctttatgga aagataaagc attgcttccc
     1621 atgttatggt tgtgccgatt tattgcctta ggtttaatga tattttgtgg cattatttat
     1681 cgctttcatg atcgcaacaa gtacgaaagg aataaagctt atgtgttgat tgccaatcac
     1741 cgttcgaatc ttgacgcgcc tgttgctgct atttcgggta aaggacgtgt gcgttatttg
     1801 gcgaaaaaag aattgctgaa ggtgccggtg atgggacaga tattcgccgt gatgtcggtt
     1861 tatgtagatc gaagtagtcc gcaagaccga cgcaaaagtg ttgagcgact gaaggaatgt
     1921 gtaaaatcgg gcgacagtat ttttcttttt cctgaaggaa caaggaataa aacagatcaa
     1981 cctatgatcg attttaagga tggcgcattt accattgcag ttcaaacgca gacaccaatc
     2041 ttgcccatgt tatatatcaa cacggataca ttgatgccaa atagtaagcc attgatgcga
     2101 cccgggatta ttgacgtgta ttatttgcct cccatagaag ttgcagggct tacagacgct
     2161 gatattcctg ctttaaagca aagaacaagg gatatgttga tgttgcgtta tcttgaactg
     2221 agtggcgata aataatttgg aagataaact tcagcaatta actttaccat acaaacacga
     2281 aatatgcaat tacaattttg cggggctgcc ggaacggtta caggaagtgc acatttgctt
     2341 acacttgatg atgggactaa gattcttttg gattgcggat tatatcaagg caatgaacct
     2401 gagtatgcga attttaatga gcgattttta tttcgccctg atgaaataga tatcgtagta
     2461 ttatcgcatg cacatattga tcactgtggc aggttgccca agttggtaaa ggatggtttt
     2521 cgtggaagga tttatgccac acatgccacc agagatctga ctgcaatatt gttgttggac
     2581 agtgcgcaca ttcaggacag agaaagcagc tttacaaatc gccgtcgcaa ggatgatcat
     2641 gaagatgagc ccttgtatga tgctaaagat gttgcagata caatggaatt atttcgcacc
     2701 gtgggatata acaaatggtt cggtatacat cctgatgtag aagttgtata taaagatgcc
     2761 ggtcatattc tcggtagtgc aactgttaca ttgcgtataa agcgacacgg catgcgtgat
     2821 tatttactcg gcttcactgc agatatcggt cgtccggatc gtccaatttt gcgagatccc
     2881 gtgccaatgg atgcctgcga ttttttaatt tgtgaatcta cttacggtga tcgtttacat
     2941 gaggaagcac cgcaagaaaa agaacatttc ctgcgcgtga ttcagcaaac ttgcgtaaca
     3001 caaaagggta agttaattat acctgcattt tcagtgggtc gtacgcaaga gatcgtgtat
     3061 atgctcgatc agttagtaag ggagaataga ttgcctgatg tgccggtata tgtcgattca
     3121 cctttggctg ttaatgcaac gcgtgttttc gagatgcatc cggaatgttt tgacaaagaa
     3181 attctggatt atatggcttt agatcctaac ccatttggat ttgagaaatt aaaatatacg
     3241 cgatcagtag aggagtcgaa agcgatcaac aataaaaaag gatgtatagt aatcagtgct
     3301 tcaggtatga ttaatgcggg gcgtgtaaag catcatgtat ttaatgccat tgaacatccg
     3361 gagaatacaa tattgattgt cggatattgt gcagataaca ctcccggcgg gcaattgcgc
     3421 aatggggcga agcaaattcg catgtttggc gaatataaat cggtgaatgc gcacgtagaa
     3481 attatgagga gcttttctgc acatggcgac taccgcgaga tgatagattt tttacgcagt
     3541 caggaaaaag ataagctacg caatatcatg ttggtgcatg gcgacaaaaa tgcactaacc
     3601 ggctttcgcg atcatttaca tgaggcgggt ttcgggcagg tggatattcc gaagcttgga
     3661 gataaggttg atttgtagtt gacaaatcaa ctgcgattca tttaaaattt ttattgtacc
     3721 aatcgtcagc agttccttcc tcaattgctt tcaccagctt ttccatcttt ttatcctttt
     3781 gcatttgagg ttgtttttga taggctatgg ccatgtcctt cagagctgaa atatgtttgg
     3841 cctgaacatc cttactgtaa ttattttcga gtacatattt tgcgcatgaa gccatataca
     3901 ataccagcat attgggattc ttttcattaa attccatgat ggttttgttt atttcaacag
     3961 ttacggtggg acttccatta acccatttga taacgaattg cgaagcttct ttgaattttt
     4021 ctacatcggt agtcggactt acatcgcgca gccaattagc ggcttgtatg atatcggctt
     4081 cgtaaggtac ataatcttca gccccttcaa gcatgtaatg acgtggaact tcccaatcct
     4141 gggcatgcgt tgaaattaca aaaaggctga tcgcaaaaaa ggaaagtagg tgtttcatgt
     4201 tataagtttt cgctaatgta cataaaatag cccataagtg aatacgggct atttttataa
     4261 cgtataaact gattaattag tctcagttac aaaagtaact tcaacatcag attcgccggt
     4321 tgaaatgctt ccatctttga tgccgggttg gtgttttaat actacggtta cagtccccgt
     4381 tgaagacgta ggtccaaccg caacattcat gtcaagacca ataggtaagc cgttggcatc
     4441 agtatcatta tagtcaaatg taatatctgc tccatcaacg gtgtaaaata attgatggtc
     4501 tgtagactct tcgtcaatct ctgcagttaa atcaagagct gaagatacac tttcatttaa
     4561 tattgatacg gtcaaatgat aatagccgtt attcagtcga atggtatcaa attcagaagg
     4621 ggcatcacca cccggaccat caatatcccg aaatgcaaag gtggatgttg acaggtcggc
     4681 attgttttca caagacagca ccacgcttgt gattaattcc tcttcattta ccgggtcagg
     4741 agtttcctca ttcttacaag agtttattga aatcagaaag ggcaataata tgaacaaata
     4801 tgttatcttt ttcataataa tattattata ggttgattga attataaatt tgattttcct
     4861 gtgattggga ttttgattct taatgtgata tttcgaccca tgtcatcggc aaaatatcgg
     4921 aatctgttca tgtaatttcg atatgcggta ttcaatagat tgtcggcact caacgaccag
     4981 ctaatttcat tagcatgtaa ggcaataaca ccttcgattc cggcagaaaa gagggtataa
     5041 gcagatggag gagggacata gtcttcatcc agaggagctc ttttttgttc aaagacatat
     5101 tcaacaccgg catgaatcgc gttatcatgc atccatttaa aatcagcagg ttgatattcg
     5161 aggtcgaatg aaattttatc tgccggcatc atcaccaacc atttattatt tgccacatct
     5221 cgtgcgcgca atgttgatgc ttttatttca gcttttaatt gtggaatgat catgtagctg
     5281 gtttgtacat cggcacctga taaattggca tttgtttgca tgtaatgaaa aaccggaaat
     5341 gcaccacgaa ttgtcaattc agcgggtagg gtaggttcga ggtaaataaa attataaatg
     5401 aaattattgt acacaccgat atcaaaatac catttatcgc tttgatgttc aattccagcg
     5461 ataatctgat atgcccgttc gctcacaaga ctatcattcc catattccaa cgaagcagaa
     5521 ccatgatgta ggccatcgct atataattca ctaacactcg gcgaacgcca tgcggagctg
     5581 aaatttaggt tgtattgtgt atgtcggcca tcgaaaaatt gcattcctgc aattccactt
     5641 aaattaccat attggtgtgt tggtgtttca agtgtattgc cttcgtatct gaaaatctgc
     5701 atccatttgt aatcataacg gatgccgcct tccaataaca ctctgccgaa tttataattt
     5761 tcaatggcgt aaattccccc actatagttt ttgaaatttg gaatgaaaat gctgtatcgt
     5821 gtgatatttt gttgattcat tccgcttatc ccaataactg cattatggcg ctttccggga
     5881 tgttgttccc attttaaatc agcggtaata gtgccgatct caaagtacaa tgaaggcaat
     5941 ccaagtgccg caatactgtc gatcagtgac gtatgtgcat catattcact tcgcaaatca
     6001 ttttgccatg cggtaattaa tgtgaatgtg cccaagcgct catggtgaaa attggtttca
     6061 tttttaatga gatgatgtac aatgtcctga tatggtctgc caattatgta tgtaaaatcc
     6121 tggtgtacta gcggcgtgtc agatgcaaaa gcagtttgca gatcggtgag gttgccaata
     6181 tgtgaaccgg agaaaatacc taccttgtta tggaagtaac tataataaac ctgactgtgc
     6241 aaattatttc tgtggtagtc aatagttgct gcaccggatt gttccgctaa agcactattt
     6301 gcgaggaaat aatttggagt ttttacattg ccctcacgct ttattgatcc ttgcagacga
     6361 taagaaaggg cggctgaatc cgatgctttg tattgcaaca tcagcgaagc ggcaccacca
     6421 cgtgtatttg aaaaacctac agcatgcaaa tcgcctgaaa cgcctttttg agttggcatt
     6481 ggcgcaggtt gaacaagaat aacaccaccg agggcatcac ttccatattg cacagatgca
     6541 gcacctttaa taacggtgag actgttggca ataaatgggt cgatctccgg gccatgttca
     6601 tttccccatt gttgtccctc ctgacgcacg ccattattca taattagtat ccgattgcta
     6661 tgcaagccat gaattaccgg tttggaaatg ccatttcctg tttgaaggct actcacacca
     6721 tttacatcct ttaatgcttc gcctaatgat ttgccggaca atcggtctag tttttttccg
     6781 ctaagggttg tttcagttaa cgtacctgta tttttatctt gttcaccatg cacaacaata
     6841 ctttcaagat tttcacttgt gtgatcgagt cggaattctt taaaagtatc ttcagttagt
     6901 gctatagtaa atgtagaagt ggtacatccg atatgagaaa ttaccaatgt aaaagtatcg
     6961 ggacataaac cttcgaacat gaatttgcca ttttcatcag ccacaacggc tgtttgggtt
     7021 tcattgatgt aaagcgttgc ataggataga ggttctttat gatccatatc cacagcgcga
     7081 ccgctaaacc gaagattaca attgtcggat tgagctttga gttgagtgag tgccgacatc
     7141 atgacacaca cgaataaaac atgtttcata cacgaaataa aaagtgatcg gccaaagcca
     7201 aataaattac agtagattaa actgtaaatg gcggcccgcg aagatcactg tagccctgaa
     7261 aaccgggttg gtgtacacta ttgagatgag caacgggaat gagatcatag gagaaggatc
     7321 ccggtatcag tacaacacgt tcttcatgta aagtaacaga tacttccaga tgacacaaga
     7381 agcagtagtt cagctcatgg atatgcggtt gtctatgatc ttgtttgcag cattcgcgac
     7441 tgtcagaacc gtattgatgc gaaacatcat gtgcgaaggt gaagtgcacg aggtctttgc
     7501 cacttagtgc tcccaacagg agtatagttg cgattagaga tagagccgat gaccgtcgca
     7561 aaaactgcat cattgtgcaa aagtaggcta ttgttacatt ttatgacaga attttgaggc
     7621 ttgcctcgca gttgtgagct taaaccttac ctgttttaac aagccttaac ctgatttaac
     7681 cggccgaccc tgcatttagg cgcagggaat tttatctttg cgccaccaaa caataaaaca
     7741 tgagaaaata ccctttatgg cttctcgccg cccttatggt gtcagtaagt gcctgcaaaa
     7801 gcacaaaagc acctgaggaa gatgcttatc aagcatttga cccggcgtct cttgattcaa
     7861 ctgtgagacc cggagatgat ttttatgcat ttgtcaataa caagtggatc gcggcgcatc
     7921 ctattcctgc cgacaaggct aaatatggtg cattttatat gctcgacgat aatagcttga
     7981 ccacattgag gtcggcaatg gaaactgctg caaaggcaaa tgctgctaaa ggcacaaata
     8041 cgcaaaaagt aggtgatttc tggaccagtg gaatggatac taatgcgatt gaaaatgccg
     8101 gttatacgcc aataaagcct tggcttgaca aaattaatgc cattggtaac gcggatgagt
     8161 tgatgcgcac agttgcacaa atgcatcgca cgtatacatt cgcaatgttc accaactggg
     8221 ttggacagga tgataaaaat agtgctgatg tgatcatgaa tatgtggcag ggcggacttg
     8281 gtttgcctga tcgcgattat tatacacgca cagatgctaa gagtcagcaa attcgtgcag
     8341 attatctggt gcatttgaaa aatgtattta tgttgatagg cgctgatgat gctactgctt
     8401 ctaaaaatgc caataccgta atgagtattg aaactgcctt ggcaaaggca agtatgacaa
     8461 atgtggagat gcgcgatccg aataaaattt accacaagat gccattggct gattttcaga
     8521 aactcacacc aaacatgaat tggaatgtgt attacactga attgaaaacg cctgaattta
     8581 aaaccggatt gaacgtagcc caacctgatt tttataaggc attgaatggt atgatgaaat
     8641 ctgtttcttt gaatgattgg aaaacatatc tcacgtatca ttttgtggat ggagcatcag
     8701 attatttgag tgcgccattt gtgaatgaac atttcgattt ccatggtaaa aaactcaatg
     8761 gcattgaaga attgaaacca cgttggaagc gcgtagttga gactgccgat ttttgtttag
     8821 gcgaagcact gggagaagaa tatgtgaaag ttgccttcag tcctgaaagc aaagagcgca
     8881 tgttggaaat ggtaggaaat atcaagacag cattgggaca gcgcattcaa aatcttgagt
     8941 ggatgaccga cagcactaag caacaagctt tgcataagtt aagcacattt actgtaaaga
     9001 ttggatatcc tgacaagtgg agagattatt ctaatcttac aattacacgc gatgcatatg
     9061 tattgaatgt gatggcagct gctgaatttg aagcgcaacg caattacaat aaaatcggtc
     9121 agccggttga taagtcggag tgggggatga caccacaaac agtgaatgca tattacaatc
     9181 catctaataa tgagatcgta tttcctgcag cgattctgca accaccattc ttcgatccta
     9241 aagctgatga cgcccttaat tatggtggaa ttggtgctgt aataggacat gagattaccc
     9301 acggatttga tgatcagggt cgtatgtatg atgcaaatgg taacctgaat atctggtgga
     9361 ctgataaaga caattcacaa tttgttgagc gtgctaatgt tgtggttaca caattcaatg
     9421 gttattgtcc gcttgacagt ctttgtatca atggtgaact tacccttggc gaaaacattg
     9481 ctgatttcgg tggtattact actgcttatc aggcttatac gatgacagaa gaattcaagg
     9541 caggtaagtc gatcgatgga tttacgccac agcaacgttt cttccttgga ttcggtcgca
     9601 tttgggccgg agattaccgt gaggatgcca tgcgtacaca attgctcact aacgtgcaca
     9661 gtccgggaga atggcgtgta aaaggaacct tagtgaacat acctgagtgg tatgaagcgt
     9721 ttggagtgaa ggaaggggat aagatgtatt tggctcctga tcagagggct aagatttggt
     9781 aatttgaaag ggaagatgat attgagacgg cctccgggat gggggccgtt ttttttgtct
     9841 aaacctttcc cgtcttaaac ctaacacgtc tcaaacctgc caggttttaa acctaacagg
     9901 tttgaaacct aacaggtcta agacctgttg atgctctttt tgaaagactc actcccaatc
     9961 tcctcaaagc ccgagaaacc gcttatatcc gattactcgc ga
//
LOCUS       DAOQOU010000122         9903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3616_length_9903_cov_5.514318, whole genome shotgun sequence.
ACCESSION   DAOQOU010000122 DAOQOU010000000
VERSION     DAOQOU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_9903_cov_5.514318"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLM90_10490"
     CDS             complement(<1..645)
                     /locus_tag="PLM90_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HMZ89847.1"
                     /translation="MTILLFFADLLVGSTPITISQLFANLLGKQDNDDINTIISLFRL
                     PKAYTAILVGAALPVAGLLMQTFFRNPVAGPDILGVTAGSSLFVAIVMMTGGLFFGID
                     AFYGMTVVLSAIAGAVVLLLIILLVAARLRDTMTLLIFGIMFGMAISAIVSIIQYYSE
                     RDALRLFMLWTFGSLGGVTHAQLIIMVPLIVFGVLTAWLLSSKLNVLLLGEQYAL"
     gene            complement(687..1802)
                     /locus_tag="PLM90_10495"
     CDS             complement(687..1802)
                     /locus_tag="PLM90_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ89848.1"
                     /translation="MRLSLVAILICLLTTGCRPTDSTPELPHGKRVAVQFARGFIIEQ
                     IGDSYLVTVRDPLDTMHILATYRLKHDNKKGKDQINIPVSRLVSFSTTHIGFLDVLGV
                     QNTLVGFSGTTYICNDSVRKMVDEGKIVEVGNEGGIDHEKIVMLHPDIVMTYLTGNAA
                     YDQVDKLYTLGLQPVINNEFMELSPLGQAEWIKFIAVFYDRLDEANAIFDKVMTEYIA
                     TSSLVGERGDRPTVFTGMAYKGEWTIPGGKSFAAGYIHDAGGKYLWESDSKTGNFPVS
                     FETMLSIAGEAEIWLHPGASASSAEILAADERYASFDALKTGHIFNNNARLCEGGGND
                     YWERGIVFPNEVLADLVAIFHPEVLKDHTFTFYTHLR"
     gene            complement(1852..3135)
                     /locus_tag="PLM90_10500"
     CDS             complement(1852..3135)
                     /locus_tag="PLM90_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89849.1"
                     /translation="MKKFMYLILAVMITTSAFSQAYVSRVLVLNEGYFDYITNDIIVP
                     VTVGAYDPQTDTYTTIQTIDEARFASDIAIDGDYYYVAADHFLQKYDLLTNALISSID
                     VEGIRQIAITNDEIIVTRGEYLVTLPSYIQVYQKETMTLDYEIPADVIPHTTEGVIVK
                     DGKAYIAVNNGFVFGGEVGYIAVLDLATAYVENIIDLGADGINPDNLMIEGDQLFTLN
                     NKDFTGSSVSSYKIGTGELATTNLANISSGCGTSAISGGSIYYQELFGTTISKFDPAT
                     ETITDENEYGISFYGLSFDPLNGQMYTSETDYFSYGKVYVYNTDGTLSTSFDAGVSPG
                     NFAFDVRMESGVKDLKSDFLKMQPNPATEQISLKADFNIETVSISNIQGASCLTLDGS
                     HADLMLIDITKLPAGQYILSATGDSSTQSAMFIKQ"
     regulatory      complement(3222..3427)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3731..4744
                     /locus_tag="PLM90_10505"
     CDS             3731..4744
                     /locus_tag="PLM90_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89850.1"
                     /translation="MKDTLPQENPYQLDFIGRLKGMLPAHISLADQLIDILNISQDSA
                     YRRIRGETAMSIDEAITLCNHYKIPIAYFTDEIKGLVNFRYVAPDGTKASFIKHIEMI
                     YKSSDELLNHEDRRIQYSAIDTPLFLQFAFEPLAKFKMHFWMREIEGIDDFKPHYDPN
                     DIDRELVTLANKIHDVYLMIPSDEIWPDDIMHTTLKQLHYYHEADYFKKKEDLEEIIA
                     QLNHLIDHTEAMASISRKLKYGDRLKQPYQEFNLYQSDVLLGNNTVLTFGGGKTTSYI
                     SHMTFSILSTNSARYASETEAWMKSILNKSNLISGISEKNRSKYFRHLRKVVEIAREK
                     MLA"
     gene            complement(4745..5032)
                     /locus_tag="PLM90_10510"
     CDS             complement(4745..5032)
                     /locus_tag="PLM90_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89851.1"
                     /translation="MTLGALTDKLRKRIVEFRWKHRFETKVSPAINFIEMALNITELA
                     MVKSRPIDILEEHWFHEGWPVIHTLEDTEWEDIIDLYRTLTFKVQERNWFR"
     gene            complement(5087..7351)
                     /locus_tag="PLM90_10515"
     CDS             complement(5087..7351)
                     /locus_tag="PLM90_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ89852.1"
                     /translation="MKRLTALVSALFITSLSSLLSAQNCTVAGVVTDKSNEEPLIGAT
                     ITLQGASVMRVSTDLDGAFKMMVAEGKYQMIVAYIGYQNDTISITASPGKEIILPIRL
                     EEFAGLLNTVVVTGSKFEKKIGEETVTIDVIKTDLIEHTNDFRLDQTVQRMPGVSVID
                     DQANIRGGAGYSYGAGSRVLILMDDLPMLTGDAAFPSWDFIPIENIQQVEIIKGASSA
                     LYGSSALNGIINVRTVFPTSTPETKLSIFNGVYFSPKDTAQKWWGDDMPFTAGGSFAH
                     KQKFNRFDLVTGAYVFNQESYLKEIYNHRARANANMRYRINNNFVLGLNVNAQVNRSA
                     NFFFWNNIDSGLYIPAPNTASFNRGFKITVDPYLNYYDAKGNRHKILTRYYGNHNVTP
                     GIDQSNFNDLFYGEYQYQKHLEDIRLVITSGVIGSKTYSNADLYGDSSYTSGNEAAYL
                     QFDKKLGNKWNLSAGMRYEFNQIGELKESRPVFRAGANYQIAKYTFARASWGQGYRFP
                     TIAEKYISTGISGLSIFPNPDLTSETGWSAEAGIKQGVKFSNWNGLMDISGFMSEYYD
                     MMEFTFGYYPTPGFMSLNIGDTRIMGAEISFIGEGKIGNIPTTLIAGYTYIDPKFQVF
                     DSLQNALSSADYNVLKYRFRHTVKFDVESTFHKFRFGAACNYTSFMEAVDAAFVDPII
                     PAIGLYIIEGLQEYRDEHDSGDYVIDLRAAWVFNDRNEISVNCNNLLNREYSVRPAKM
                     EAPRNIMVKYSLNL"
     gene            7467..8258
                     /locus_tag="PLM90_10520"
     CDS             7467..8258
                     /locus_tag="PLM90_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ89853.1"
                     /translation="MKKLLWIVIPAMLITACTRVPITNRKQVHLLPESQMISMSLAAY
                     SDFLKESDVVDAGKDNNSVHRVADKLIPAVTKVLNDEGYGDLIKGYQWEVNLVESKEP
                     NAWCMPGGKIVVYTGILPYTKDDAGLAVVLGHEIAHAVARHGNERMSQGLLAETGFMA
                     LDVALANKPTETRNLLMTAVGVGTQVGVLLPFSRTQESEADRLGLIFMAAAGYDPHNA
                     INFWERMSANSAGIPEFLSTHPSDETRITNIRDTYMNEAMKYYSK"
     gene            8268..8945
                     /locus_tag="PLM90_10525"
     CDS             8268..8945
                     /locus_tag="PLM90_10525"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HMZ89854.1"
                     /translation="MALKAISPWQRIFSYFKPIQLTTQHSEFTPVLEVVLTEGRCMLN
                     SPDATYSFEDKYTSYRTAIHQIRNRLKNYNHILVLGLGLGSVPYMLQTKFAFKGKITC
                     VEIDPVIVSLAKHYYPNKQLLQKLDLFTDDAVHFMEHNKLQFDAIFCDLFIDKRVPAA
                     LHEFDFLHHVKSALAPGGTLLFSRLASERQAELTLWENLKQVFPEGEDIDTGGNYILN
                     YQAPITT"
     gene            8942..9895
                     /locus_tag="PLM90_10530"
     CDS             8942..9895
                     /locus_tag="PLM90_10530"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ89855.1"
                     /translation="MTGNDIGFAAQKLREGALVAIPTETVYGLAANAFDKEAVTRIFE
                     AKQRPQFNPLIVHTSDIERVNSFTTGISGVLEQLATTFWPGPLTILLPRNHMIHPLVT
                     AGSDFVAVRIPNHPMTLALLRNLEFPLAAPSANLFGRVSPTTAAHVEEQLGEKVSYIL
                     DGGNCAVGVESTIVKLKNDGVVSILRPGGVSAEMMHPILGYEPEFSTPQGDRPEAPGM
                     LKSHYAPKIPLVLGEVAAMLEKYKQQRVCLITFSEGIAHSSVIRNIILSGRGDLHEAA
                     QQLFSALHEAENEDVEIIIAERVPEIGIGIAINDRLERASA"
BASE COUNT         2945 a         2177 c         1972 g         2809 t
ORIGIN      
        1 caaagcgtat tgttcaccca gcaagagcac attcaatttc gatgaaagca accatgccgt
       61 caatacaccg aatactatta atggcaccat tataattaat tgcgcatgtg taacaccgcc
      121 caggcttccg aatgtccaaa gcataaacag gcgcaaggca tcccgttcgc tgtaatattg
      181 tattatgctg acaattgcgc tgatagccat tccaaacata attccgaata tcagtaatgt
      241 catggtgtct ctcaatcgcg cagcaaccaa aagaataatt aataataaca caactgcacc
      301 tgcaatagca gacagcacaa cagtcattcc ataaaaggca tcaatgccaa aaaacagacc
      361 acctgtcatc attactattg caacaaatag acttgaccct gccgttacac caagaatatc
      421 gggacctgca acagggtttc tgaagaatgt ttgcatcaac aatcctgcaa caggcaatgc
      481 agcacctact aatattgctg tatatgcttt aggaagtctg aataaggaga taatggtatt
      541 gatatcatca ttatcttgtt tgcctaacaa atttgcaaac aactgagata tcgtaatcgg
      601 agtagagcca accaggagat cagcgaagaa taataatatt gtcaagacca acaatatcgt
      661 caaaggccat atgccctgat ttcgcttcac cttaaatgcg tgtaaaaggt aaatgtatga
      721 tctttcaaaa cctcaggatg aaatatggct acaagatcgg cgagtacttc atttggaaaa
      781 acaattccac gttcccaata atcattaccg cctccttcgc ataaccttgc attgttgttg
      841 aagatgtgac cggtttttaa agcatcaaaa gaagcataac gttcatcggc ggcaagaatt
      901 tctgcacttg acgcagatgc cccggggtgc aaccaaattt ctgcttcgcc tgctatactt
      961 aacatggtct caaagctcac cggaaaatta ccggtctttg aatccgattc ccataagtat
     1021 ttccctcctg catcatgaat atatcctgcc gcaaaactct ttcctccagg aattgtccat
     1081 tcacctttat aagccatgcc ggtaaaaaca gttggtctat caccacgttc accaaccagt
     1141 gatgaagtag caatatattc agtcattact ttgtcgaata tcgcgttagc ttcatccaac
     1201 ctatcataaa acacagcaat aaatttgatc cattccgcct gtcctaatgg cgacaattcc
     1261 ataaattcat tatttatcac aggctgtaat cccaatgtat acagcttgtc cacttgatcg
     1321 taagccgcat ttcctgtcag gtatgtcatg acgatatcag gatgcaacat cacaattttt
     1381 tcatggtcga ttccaccttc atttccaact tcaactattt ttccttcatc aaccattttt
     1441 ctcaccgagt cattgcagat ataggtagta ccactaaatc caacaagcgt attttgaaca
     1501 ccaagtacat ctaaaaaccc gatatgcgtt gtagaaaaac taaccagtcg cgatacggga
     1561 atattgattt ggtctttacc ttttttgtta tcgtgcttta acctgtacgt agccaggatg
     1621 tgcattgtat ccaagggatc gcgcacagtg actaaataac tatcacctat ttgctcaata
     1681 atgaatccgc gagcaaattg cacagctaca cgtttcccat gaggtaactc cggggttgaa
     1741 tcagttggtc gacaaccggt agttagcaaa caaatgagta tggcgacaag tgagagtctc
     1801 ataaaaatca aacgggtaaa accatcaata gtcttacccg tttatcatgc attattgttt
     1861 aataaacatg gcgctctgag tacttgaatc accagtagcg gaaaggatat attgtcctgc
     1921 aggcagttta gtgatatcaa tcaacattaa atcagcatga gaaccatcca aagttaaaca
     1981 agaggcacct tgaatattgc tgatacttac tgtttcaata ttgaaatctg ccttcaggct
     2041 tatttgttca gttgcaggat ttggctgcat ttttaggaag tctgatttga ggtcttttac
     2101 acctgattcc atcctgacat caaatgcaaa atttccggga gacacgccgg catcgaagct
     2161 tgtagacaat gtaccatcag tattatatac atatactttt ccatagctga aatagtctgt
     2221 ttcggaagtg tacatctgtc cattgagcgg gtcgaagctc aatccgtaga aactgatgcc
     2281 atattcattt tcatctgtta tcgtttccgt tgcagggtcg aacttactga tggtagtgcc
     2341 aaataattcc tgataataaa tgcttccacc ggatatagca gatgtgccac atcctgaaga
     2401 aatatttgca aggttagtag ttgccagttc gcctgttccg attttgtatg aggaaacaga
     2461 acttcctgta aaatctttgt tgttgagtgt aaacaattga tctccttcga tcatcagatt
     2521 atccggattg atgccatcag cacccaggtc aatgatattt tcaacatatg cggttgcgag
     2581 gtctaatacg gcaatatatc ccacttcccc accaaaaacg aaaccgttgt taactgcgat
     2641 gtaagcttta ccatctttta cgataacacc ttcagtagtg tgcggtatca catctgcagg
     2701 aatttcataa tcaagcgtca tcgtttcttt ttgatacacc tgaatatatg atggcagcgt
     2761 taccaaatat tcaccacgcg taacaatgat ctcatcattt gtaattgcga tttgacgtat
     2821 gccttcaaca tcaatgctgc tgatgagggc gttggtaagc aaatcatatt tctgcaaaaa
     2881 atgatcagca gccacataat agtaatcgcc atcaatagca atatcagaag cgaacctggc
     2941 ttcatcaata gtttgaatgg tagtataagt gtctgtttgt ggatcataag cgccaacggt
     3001 caccggaaca atgatgtcat tggtaatgta atcgaaatac ccttcgttca acacgagcac
     3061 tcgcgaaacg taggcttgag aaaatgcgga ggtggtaatc atcaccgcga gaatcaggta
     3121 catgaatttc ttcataatag gttgtttttc taaatgaaat aatagaggca atctattaat
     3181 acagcatgtt cgctgtgcca catccctttt gccccaaaag agatgatcgt gcaggcattg
     3241 gcaggtcttc tggctcttcc gcccttcgga aaaccttccc attcaatcaa ttgaacagtg
     3301 gtattgcgct tccgaaagca tagactgcgg aattacagca gtggggactg ccccggattc
     3361 acaccgggtt ccctcttagg gcaaccttcc gtatcggtta gtcgccacca atcttgcatg
     3421 gtaaagatat atagcccttc tgccaccgat aaacagagtt atacacaccc tatgcaaaag
     3481 ccttgcaacc tttgaaataa aaatatttga gcagtacact aatctgaaag acgtgcacgt
     3541 tttagtgctg tcttgtcgca gagacgttca agtaagaaaa ggcgctacgc cccaggggtg
     3601 tggcgccctt acattttata aactttccca ttttttcatt aatcaaaatg catcaggtca
     3661 tcaattacat tttgttcaaa aatgggtacc atcattgcac atatcatgca tttttcgcaa
     3721 attgcggacg atgaaagaca cactgccaca ggaaaatccc tatcaactgg attttatcgg
     3781 acgattaaaa ggaatgctgc cggctcatat ttctttggcc gaccagttga ttgatatcct
     3841 caatatcagc caggatagtg cctaccgacg cattcgtggt gaaacagcca tgagcattga
     3901 tgaggctata acattatgta atcattataa gatacctatc gcctatttta cagatgaaat
     3961 aaaaggcctg gtgaacttcc gttacgttgc tcctgatggc accaaggcat cgtttatcaa
     4021 acatatagaa atgatatata agtcttctga tgaattgctt aatcatgaag accgtcgtat
     4081 ccaatattct gccatcgaca ctcccctatt tcttcagttc gcatttgaac cactcgcgaa
     4141 atttaaaatg catttttgga tgcgagaaat agaaggcatt gatgatttca aaccacatta
     4201 cgatcccaat gatatcgaca gagaattagt gaccctcgcc aataaaattc acgatgtata
     4261 cctgatgatc cccagcgatg aaatttggcc tgatgatatc atgcatacta cgttaaaaca
     4321 attgcattac tatcatgaag cagattattt caaaaagaaa gaagatctgg aagaaattat
     4381 tgctcaactc aatcacctga ttgatcatac cgaagctatg gcatcaatta gcagaaaatt
     4441 aaagtatggc gacagattaa aacagcctta tcaggagttc aatttatatc aaagtgatgt
     4501 gctcctgggc aataatactg tactaacctt tggcggagga aaaactacaa gttatatcag
     4561 tcatatgact ttcagtattt taagcacaaa cagcgcacgc tatgcctctg aaacagaagc
     4621 ctggatgaag agcattctca ataaatcaaa tctgatcagt ggcatctctg agaaaaaccg
     4681 ctcaaaatat ttcagacatc ttcgaaaggt agtagaaatt gctcgcgaga aaatgctggc
     4741 ctaattaacg aaaccaattg cgttcctgca ctttgaaggt aagggtgcga tacagatcaa
     4801 taatatcttc ccactctgta tcttctagtg tatgaatgac aggccagccc tcatgaaacc
     4861 agtgttcttc aagtatatct ataggtctac ttttcaccat agccaattct gtgatattca
     4921 gtgccatttc gataaaatta attgcaggcg acacctttgt ttcgaatcga tgcttccatc
     4981 tgaattcaac gatgcgcttc ctcaatttat ctgtaagggc tcctaaggtc atggctctgt
     5041 aaactataat cctaattcac taatagtaaa tagtattaaa tcgcaattaa agattcagtg
     5101 aatatttcac catgatattt cttggtgctt ccattttcgc agggcgaacg gaatattcgc
     5161 gatttaataa attattacaa tttacactga tttcattccg gtcgttaaac acccaggcag
     5221 cgcgaaggtc aatcacataa tcgccggaat catgttcatc tctgtattcc tgaagtcctt
     5281 caatgatata cagtccaatt gcaggaataa tcgggtcaac aaatgcggcg tcaacggctt
     5341 ccataaaact ggtataattg catgctgcgc caaatctgaa tttgtggaag gtgctctcaa
     5401 catcaaattt cacggtatga cggaatctgt atttcagcac gttatagtcg gcactggata
     5461 atgcattttg caatgaatca aatacctgaa attttggatc gatataggta tatcctgcaa
     5521 ttaaagtagt aggaatattt ccgattttcc cttcaccgat aaaagaaatc tctgcaccca
     5581 ttattcttgt atcgccaata ttcagcgaca tgaatcccgg tgttggataa taaccgaaag
     5641 tgaattccat catgtcgtag tactccgaca taaatcctga aatatccatt aatccattcc
     5701 agttagaaaa cttgacacct tgcttaatac ccgcttctgc actccagcct gtttcgcttg
     5761 tcaaatcagg atttgggaaa atactcaagc ctgaaatacc ggtcgaaata tatttttcag
     5821 caattgtcgg aaagcgatat ccttgccccc aggatgcacg agcgaaagta tattttgcaa
     5881 tttgataatt cgctccggct ctgaacaccg gtctcgattc cttcaattct ccgatttgat
     5941 taaattcata tcgcattccc gcacttaaat tccatttatt accaagcttt ttatcgaatt
     6001 gcaggtatgc cgcttcattg cctgaggtat atgaactatc gccgtacaaa tcggcgttag
     6061 aataagtttt actgccaatt acacccgagg taatcacaag tctgatatcc tccagatgtt
     6121 tttgatattg atattctccg taaaaaaggt cattgaaatt actttgatcg attccgggag
     6181 taacattatg attgccgtaa tagcgagtca gtattttatg tctgttccct tttgcatcgt
     6241 aatagttaag ataaggatct acagtgattt taaacccgcg attgaaagag gcagtattag
     6301 gcgccggaat atataacccg gagtcaatat tattccagaa aaagaaattc gccgatctat
     6361 tgacttgtgc atttacattt aaccccagca caaaattatt attaatgcga tagcgcatat
     6421 tcgcatttgc tcttgccctg tggttgtaga tttctttcaa ataactttct tgattaaata
     6481 catacgctcc ggttacaaga tcaaatcggt tgaatttttg tttgtgcgca aaactccctc
     6541 ctgcagtaaa cggcatatca tcaccccacc atttctgtgc ggtatctttc ggactgaaat
     6601 aaacgccatt aaaaatggaa agtttggttt cgggtgttga tgtgggaaat actgtgcgaa
     6661 cattaatgat accattcaag gcactggaac catacaatgc acttgaagcg cctttgatga
     6721 tctctacttg ttggatattt tctatgggaa taaaatccca gctcggaaat gccgcatcac
     6781 cggtcaacat gggcaggtca tccatcagaa tgagcaccct gcttcctgca ccatagctat
     6841 atcctgctcc gccacggata tttgcctgat catcaatcac actaactccc ggcatccgct
     6901 gaactgtttg gtccaaacga aaatcatttg tgtgttcaat caaatctgtt tttatgacat
     6961 caatggtaac tgtttcctct cctatttttt tctcgaattt agaacctgtc accaccacag
     7021 tattcagcaa tcctgcgaat tcctccagtc gaataggaag tataatttct tttccgggac
     7081 ttgcggtaat tgaaatagta tcattttgat aaccgatata agcaactatc atttggtatt
     7141 taccttcagc aaccatcatt ttaaaggctc catcaagatc ggtgctcacc cgcataacac
     7201 ttgcaccttg aagtgttata gtagctccta tgagcggctc ttcattagac ttgtcagtta
     7261 cgactccggc aacagtacaa ttttgggccg ataataacga agaaagagat gttataaaca
     7321 gggctgaaac taaggctgtc agtcgcttca tgtgtggttt tgttgacggg taaagataaa
     7381 taaacctacc tttcaggaaa cagagttctg cacacgattt acaaattgag tacgatattt
     7441 gcatgactaa cccaaaacct atacagatga agaagctgct ttggattgta atccccgcta
     7501 tgttgatcac cgcctgcaca cgcgtgccta ttaccaaccg caaacaggtg catttactgc
     7561 ctgaaagtca gatgatctcg atgagtctcg cagcatacag cgatttcctc aaggaaagtg
     7621 atgtggtaga cgcaggcaag gataacaatt ccgttcaccg cgtggcagat aagctgatac
     7681 ctgccgttac caaagtgttg aatgatgagg gctatggcga cctgataaaa ggttaccaat
     7741 gggaagtaaa cctggttgag agtaaagaac ccaatgcctg gtgcatgccc ggcggaaaga
     7801 ttgttgtgta taccggaatt ctgccctaca ccaaggatga cgccggcttg gcagtagtat
     7861 tgggacacga gatagcccat gccgttgcgc gccacggaaa tgaacgtatg agccagggtt
     7921 tactcgctga aaccggattt atggcacttg atgttgcttt agccaacaag ccaacagaaa
     7981 caagaaattt gcttatgact gccgtaggtg taggtacaca agttggtgtt ttgctgcctt
     8041 tcagccgcac acaagagtct gaagccgatc gattgggact catatttatg gctgccgcag
     8101 ggtatgatcc gcacaatgcc atcaactttt gggaacgcat gagtgctaac agtgcaggga
     8161 ttccggagtt tttgagtacg catccaagcg acgaaaccag gatcaccaat atccgcgata
     8221 cgtatatgaa tgaggctatg aagtattatt cgaagtagct ttgcaccatg gcactaaagg
     8281 caatatcgcc atggcaacgc atttttagct atttcaagcc tatacagctc acaactcaac
     8341 acagtgaatt tacacctgtt ttggaggtcg tgcttacaga aggacgctgt atgctcaatt
     8401 ctccggatgc tacttattca tttgaagata aatacactag ctacagaacg gccattcatc
     8461 agattcgcaa caggcttaaa aattacaatc acatattagt attgggattg ggcttaggat
     8521 ctgtgcctta tatgctacaa accaaatttg ccttcaaggg caagatcaca tgtgtagaga
     8581 ttgatcctgt aattgtatct ctggcaaaac attattatcc aaacaagcag ctattacaga
     8641 agctcgattt gtttaccgat gatgcggttc actttatgga gcataacaag ctgcaattcg
     8701 atgccatttt ctgcgatttg tttattgata aacgtgtccc tgcagccttg catgagttcg
     8761 acttcctgca tcatgtaaaa agcgcgcttg cgccgggtgg cacactttta ttcagccggt
     8821 tggcatcaga gcggcaggct gaattaactt tgtgggaaaa tctcaagcaa gtttttcccg
     8881 aaggtgagga tatagatacc ggcgggaatt atattttaaa ttaccaggca ccaattacaa
     8941 catgacggga aatgacattg gttttgcggc gcagaagttg cgggaaggcg ctctggtagc
     9001 cattcctacg gaaacagtat acggactggc agccaacgcg ttcgacaagg aggccgttac
     9061 acgtattttc gaagctaaac aaagacccca attcaatccg ttgatcgttc acacctcaga
     9121 tattgagcgg gtaaactctt tcacaacggg catttcaggc gttttggagc aattggcaac
     9181 aaccttttgg ccgggaccat tgacgatcct gctaccgagg aatcacatga tacatccgct
     9241 tgtaacagcg ggaagtgact ttgtggcagt ccgaatacca aatcacccaa tgactttagc
     9301 cttgctacgc aacctggaat tccctctggc agcgccgagt gctaatcttt ttggcagggt
     9361 tagtcccaca actgcagcgc atgttgaaga gcaattaggc gagaaggtat cctatatttt
     9421 ggatggtgga aattgtgcgg taggggttga aagcaccatc gttaagctga aaaatgatgg
     9481 ggttgtaagc atccttcgtc ccggtggtgt ttctgccgaa atgatgcatc ctattttagg
     9541 gtatgaaccg gaattcagca caccacaagg tgatcgccct gaagcgcccg gaatgctgaa
     9601 gagtcattat gcgccaaaga ttccactggt tttgggagaa gttgctgcta tgctagaaaa
     9661 atacaagcaa cagcgcgttt gcttaatcac cttttccgaa gggatagcgc attcgagtgt
     9721 tatccgaaac atcattttga gtggtagagg cgatttacat gaggctgcac aacaattgtt
     9781 ctcagctttg catgaggctg aaaatgaaga tgtagaaata attatcgccg agcgtgtgcc
     9841 tgaaatcggc attggcattg ctattaacga ccgtctggaa agagcatctg cctagtggta
     9901 ttg
//
LOCUS       DAOQOU010000123         9898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3618_length_9898_cov_6.502083, whole genome shotgun sequence.
ACCESSION   DAOQOU010000123 DAOQOU010000000
VERSION     DAOQOU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3618_length_9898_cov_6.502083"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1720
                     /locus_tag="PLM90_10535"
     CDS             380..1720
                     /locus_tag="PLM90_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89856.1"
                     /translation="MRFPTLASIFESDTTSADISNIVSIDAGDDTTEVVAKVIDTANI
                     KGLVQLDSGVAIVTPIQFGTKEAMDNFYASLYKTASDGSRVRVMHYGDSQIEVDRITG
                     YLRARLQSQFGGSGPGLISVMPIADFVAVKNSWSGSWDRYTTFTNRDKRVPHRSFGPN
                     AAFSRFMPVRDTTHALGSQHSAWLKITTNKNGGARLANYRYVKLLYGNATDPVKVELL
                     ADDVLIATDTLSVGGDHNIQEFALAAAPGNFQLNFEGRDSPDIYGLSLEGNGGVIVDN
                     LALRGSSGNFFTGMNQSQLKGYYDQMDVRLIIMQFGGNALPYLKDEAGCIKYAQSIES
                     QINTMKWLAPGASILFVGPADMSVKEGTTYVTHPLLEILNTELKNAAFRAGAAYYDMY
                     AAMGGKNSMVAWVNAGIATKDYIHFSPEGARKIAILLYQAMMKEYNNYVIRESI"
     gene            1723..2931
                     /locus_tag="PLM90_10540"
     CDS             1723..2931
                     /locus_tag="PLM90_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HMZ89857.1"
                     /translation="MRQTILLLGMLMMFGSINAQTSYPFIKYDTSRIIYPGDSASMLK
                     FFAKLDALKKGDTSRLTIAHYGGSHIQAGYWTEALADSFQALGGYVGGGAYLFPYRLI
                     KTNGPPFYRSHGTGEWKRCRAVPSEMCSGLGMSGISATTSDSIASFGFQLRPNDHINS
                     FTYIRVYHNFNPDYVIEVPKDFPATYLRHDYPELGYVQFHFQTPVDSVHFDIHRNSST
                     RSDFILRGWSIENENPGVYYASMGVNGASTRSYLQCTEFVKELGTLHPDLVIFSIGVN
                     DAQDADLVLPDFFARYDSICAIVRAINPDCAILFTTITDNYIKRKTKNTRSLKVNAQI
                     MAVAQKYNAGVWDMFTVMGGLGSIDKWYRNGLAAPDRIHFNAKGYGIAAGLMFNAIKD
                     SYMYNTTLSK"
     gene            2938..4602
                     /locus_tag="PLM90_10545"
     CDS             2938..4602
                     /locus_tag="PLM90_10545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ89858.1"
                     /translation="MNNIFSNIAHWLSYDPENPLIFTRLFFWAFFAVVLLGYSFVYKK
                     VNVRTIYLLAASFFFYYQTSGVFLILLIFTIISDYNWGRLLAKTETPWKRRMFLTFSV
                     VLNLTLLCYFKYAFFFTENLNEVAGTDITFFNHFAHFTNTWFGTSFSVDKLILPIGIS
                     FFTFQSLSYTIDMYRRKLEPVKNIFDYGFFVCFFPHLVAGPIVKAHDFIYQIYQPYNL
                     SKREYGMAFFWILNGLGKKIMADYVGVNFVDRIFSHPELYSGVEVVLGIFGYSLQIYA
                     DFSGYTDMAIGIALLLGFRLKTNFRSPYKAESTSEFWKRWHISLSSWLKEYLYIPLGG
                     NRNGTVGSYVAISILGIFMVLLYGNVTLIYILLAIALVLILIARIFPKFRHSVNTNIN
                     ILVTMLLGGLWHGSSWMFIIWGGLNGLGLMLHKGWERINPIKNTSGVGYRAFAIFLTL
                     VFISFTRIWFRSDSLDTVGIIFDRISHHFGGELIENVLWGYRTVLSVIVVGYLIHWIP
                     ESTKQQYREWFATRSEPALVAILIGYIFLLYQAVSADMQPFIYFQF"
     gene            4693..8304
                     /gene="dnaE"
                     /locus_tag="PLM90_10550"
     CDS             4693..8304
                     /gene="dnaE"
                     /locus_tag="PLM90_10550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMZ89859.1"
                     /translation="MSTFSHLHNHTMFSLLDGASDIGRLYKKAAADGMPALAITDHGN
                     MFGAFQFVAEAYKHKGEDGKVKVKPIVGCEFYLVADRTRKQFSREEKDIRYHQVLLAK
                     NETGYKNLVKMCSLGYMEGMYGKYPRIDKTLIEKYHEGLIATTCCLAAEVPRTILKKG
                     REEGEKVFKWWLDIFGEDYYIELQRHDIPEQETLNNILLELADKYNVKVIATNDAHYV
                     DQQDANAHDILLCINTGEKQATPKMKDFGDDDMIIKGKRFAFYNDQFYFKTQSEMNEL
                     FHDVPQALDNTNEIVDKVELLDLKRDILLPAFPIPDAFKIHGDDTTNQWEYLKHLTME
                     GAHKRYGTMDAETEERINFELFTIKTMGFAGYFLIVMDFIRAGRELGVFVGPGRGSAA
                     GSVVAYCIGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDDGRQKVIDYVVDKYGK
                     NQVAQLVTYGTMAAKTSIKDVARVMDLPIADSNALSKLVPERPGISLKRLIQAPISGD
                     GSLSEKENLTPDELNNAKALRAIAEDQKDIRSKIIKEALILEGSVRNTGVHAAGLIIA
                     PKDLTDLLPIAVAKDSALYVTQFEGEVIESAGVIKMDFLGLRTLSIIKTALKLIKENH
                     NVIIDIDTIPLDDLKTFELYQRGETNATFQFESPGMQKYLRELKPDKFEDLIAMNALY
                     RPGPLEYIPTYIKRKHGREPIEYDLPQMEEYLKETYGVTVYQEQVMLLSQKLAGFTKG
                     DADTLRKAMGKKQKDVLDKMKSKFLEGADKNNIDKKVCEKIWTDWEAFAQYAFNKSHS
                     TCYAFVAYQTAYLKAHYPAEYMSAVLTHNQGNIDKVSFFMDECRRMGVPVLPPDVNES
                     DLNFAVNKNGQIRFGMSAIKGVGESAVIELINERKKDGAFNSLFDLTRRVSLRSVNKK
                     TFEALVYAGAFDSFGLNRSQYFELAKNETLNLIEQAIRYGNNFNGDTVKSQNTLFGDS
                     EDVEIAEPKIPDFPEWNTIEKLKKEFEVVGMYMSGHPLDDYKIEADAFKTTTITDIEK
                     FTDKGDIVLVGYVASTDTKVGKTGDKYTIFTLEDFSGHLEMGLFKNNYIKFKNYVDQT
                     GNVLYVKGAYQTDRNDPERKRFWVSDIQLLSEVREKKSMKITLSMSLSDLNSNFLEGL
                     SEVLKQHPGKDSIVLKVIEHTEQFDLLMRNKQGGIRAESAVLNSLGKFGEVNLTVVG"
     gene            8394..8711
                     /gene="trxA"
                     /locus_tag="PLM90_10555"
     CDS             8394..8711
                     /gene="trxA"
                     /locus_tag="PLM90_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ89860.1"
                     /translation="MAIEFNDANFKQTVLDSGKLSVIDFWAEWCGPCRVVGPVIEELA
                     KDYDGKVNIGKVNVDFNPEISMMYGIRNIPTILFIKNGQVVDKQVGAVPKSVLDAKVR
                     QHM"
     gene            8787..9722
                     /locus_tag="PLM90_10560"
     CDS             8787..9722
                     /locus_tag="PLM90_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ89861.1"
                     /translation="MDRQLISLQDLHQVENLELIAKQVVEGFILGLHKSPFHGFSVEF
                     AEHRIYNLGEATRHIDWKVFGRTDKLFTKKYEEETNLRCHIVIDASSSMYFPEPDKQA
                     KGLHLNKLIFSAICSSAIIHLLKMQRDAAGLTIFADDVLLHTKAGSSNVHHKMLQTQL
                     ESFIGKSSHDLPTNAGKCLHQIAENIHRRSMVVIFSDMMEDVEKSEELFSALQHLKHN
                     KHEVILFHVTDKRHEINFEYDNRPYIFVDMETGEKIKVRSSEVKEKYTEQMAKYMQQL
                     KLRCGQYGVDLIEADMMEDFRDVLLPYLVRRGKMH"
BASE COUNT         3035 a         1760 c         2230 g         2873 t
ORIGIN      
        1 gcaatactat acgaaggtta aagggaatcg ggagcggtat tcggtaagtg ttttgaatta
       61 attccttaaa ttatgttttt taatattcaa aatgttcttc agcctaaatc actgaattga
      121 agcagaagtt aaagtcgata ttatttgcag ttaaatataa taagcggcac gaatgtccgt
      181 ttaattcaac aattgtgcat tcaaatgttg gtgaatgcac acaacacatg acccacttcc
      241 gtaattttat cccctcttca caatgagcaa catcacgcct cttaggatat tggcatttct
      301 ggccatatgt ttcgtcgcac tgggcgctgt gtgtgccgta tttccggaaa aaggcatccc
      361 cttagggaaa aatgttacct tgcggtttcc tacgttagca tctatatttg agtctgacac
      421 tacttctgct gacatcagta atattgtctc aatcgatgcc ggcgacgata ccacggaagt
      481 tgttgccaaa gtgattgaca ctgcaaatat caaaggactc gttcaattgg attcaggagt
      541 tgccatcgtt acaccaattc aattcggcac aaaagaagcg atggataatt tttatgcatc
      601 actttataaa acagcatctg atggctctcg ggttagggtg atgcactatg gcgattcaca
      661 gatcgaggtc gatagaatta ccggatacct cagagccagg cttcagtccc aatttggcgg
      721 ctccggtccg ggactaatct ccgttatgcc tattgctgat ttcgttgcgg taaaaaatag
      781 ctggagtgga agttgggaca gatataccac ttttaccaat agagataaac gagtcccgca
      841 tcgaagtttt ggtcctaatg cagcatttag caggtttatg ccggtaaggg atacaacaca
      901 tgctcttggt agccaacata gtgcctggtt aaagatcact acgaacaaaa atggtggcgc
      961 cagattggca aattatcgat atgtcaaatt attgtacggc aatgctacag atcctgtaaa
     1021 agttgaattg cttgccgatg atgtattaat agcaacagat acactgagcg ttggcggtga
     1081 tcataatatt caggaatttg ctctcgccgc agctccggga aatttccaat taaattttga
     1141 aggtcgtgac agtccggata tttatggctt gtctttggag ggaaacggtg gtgtaatagt
     1201 ggataatctt gccctgcgtg gaagctccgg gaattttttt acaggcatga accagtcgca
     1261 attgaaaggt tactatgatc aaatggatgt ccgtttgatc atcatgcaat tcggaggtaa
     1321 cgcgttacca tatctcaagg atgaggcagg atgtattaaa tacgctcaat ctattgaatc
     1381 gcagatcaat accatgaaat ggttagctcc gggtgcgagt atattatttg ttggccctgc
     1441 agatatgagt gtaaaggaag gaacaacata tgtgacacat cccctgcttg aaatattaaa
     1501 cactgagctt aaaaatgcag cctttcgtgc aggcgcagct tactacgata tgtatgctgc
     1561 aatgggcgga aaaaatagta tggtggcatg ggtgaatgca ggaattgcta ccaaagacta
     1621 tattcacttt agccctgaag gagcacgcaa aattgccatt ttattatacc aggcaatgat
     1681 gaaagaatat aataattatg tgatcagaga gagtatatga acatgcgaca gacgatacta
     1741 ctacttggaa tgttgatgat gtttggaagc attaatgcac aaacaagtta tccgttcata
     1801 aaatatgata ccagcaggat catttatccg ggcgatagtg cgagtatgct gaaatttttt
     1861 gccaaattag atgccctaaa aaaaggtgat acatctcgac tgacaattgc gcattatggt
     1921 ggatctcata ttcaggcggg ctattggaca gaggcgcttg cggatagttt ccaggcatta
     1981 ggcgggtatg taggaggtgg agcatattta tttccctatc ggctaattaa aacaaatgga
     2041 ccgccgttct atcgctctca tggcacaggt gaatggaaac gttgtagagc agtgccttct
     2101 gaaatgtgca gcggattggg aatgagtgga atctctgcaa caacaagtga tagcatagca
     2161 tcattcggat ttcaacttcg acctaatgac catatcaatt catttaccta tatcagagta
     2221 tatcacaact ttaatcctga ttatgttata gaagtgccga aggatttccc tgctacttat
     2281 ttgcgacatg attaccctga actgggatat gtgcaatttc actttcagac acctgtagat
     2341 tctgtgcatt tcgatataca tagaaattca tcaacacgat ctgattttat tttacgtgga
     2401 tggagtattg aaaatgaaaa tcccggagta tattacgcgt cgatgggtgt aaacggcgcc
     2461 agcacaaggt cttacttgca atgcaccgaa tttgttaaag agcttggcac cttacatcca
     2521 gatttggtga ttttttctat tggcgttaat gatgcccaag atgccgacct tgttttacct
     2581 gatttctttg cgcgttatga ttccatttgt gccatagtga gagcaataaa tcctgattgc
     2641 gcgatacttt ttactaccat cacagataac tacattaagc gaaaaacaaa aaataccagg
     2701 agtctaaaag tgaacgcgca aataatggct gttgcacaaa aatataacgc cggtgtttgg
     2761 gatatgttta ccgtaatggg cggactaggg agtatcgata aatggtatcg caatggtttg
     2821 gctgcgccgg atagaataca ttttaatgct aaagggtatg gaattgcagc aggcttgatg
     2881 ttcaatgcaa ttaaagacag ctacatgtat aataccacat tatcaaagta attcgcattg
     2941 aataacattt ttagcaatat tgcacattgg ttatcttatg atccggaaaa tccgttgatc
     3001 tttacacgtt tgtttttctg ggcattcttt gcagttgtgt tgcttggata ttcattcgta
     3061 tataaaaaag taaatgtccg aaccatttat ctgctggcag cgagtttctt tttctattat
     3121 caaaccagtg gtgtctttct tatcctcctc atttttacta tcatcagcga ctataactgg
     3181 ggtcgactgc ttgcaaagac agaaacgccc tggaaacgaa ggatgttcct cacattcagt
     3241 gtggtgttga atttaacttt gctttgttat tttaaatatg cgtttttctt taccgaaaat
     3301 ttaaatgaag ttgccggcac ggatataact ttctttaatc atttcgcgca ttttacaaat
     3361 acatggtttg gtacttcatt ctcagtagat aaactcatac tgccaatcgg tatttcattc
     3421 tttacttttc aatcgctgtc gtataccatc gatatgtatc gcaggaaact tgagcctgtg
     3481 aaaaacatat tcgattatgg attttttgtt tgttttttcc cgcacctggt ggcagggccg
     3541 attgtaaaag cacatgattt catttaccag atttatcagc catataatct ttcgaaaagg
     3601 gagtatggaa tggcattttt ttggatattg aatggattgg gcaaaaagat aatggccgat
     3661 tatgttggtg tcaatttcgt ggatcgtata ttctcgcatc ctgaattgta ttccggcgta
     3721 gaagtggtgc ttggcatatt cggatacagc cttcagatat atgccgattt cagcggctat
     3781 acagatatgg ccattggcat tgcattatta ttaggattca gattgaaaac gaattttaga
     3841 tcgccgtaca aagctgaaag cacaagtgaa ttttggaagc gttggcatat atccttgtcg
     3901 agctggttaa aagaatacct ctatattcct ttgggaggga acagaaatgg aacagtgggt
     3961 tcctatgttg cgatatcgat tctgggaata tttatggtat tattatatgg taatgtcacc
     4021 ttgatttaca tcttgttggc gattgcttta gtattaattt taatcgcaag aatatttcca
     4081 aaatttcggc attctgtaaa taccaatatc aatattttgg ttaccatgtt gctaggtggt
     4141 ttatggcatg gaagcagctg gatgtttatt atttggggcg ggttgaatgg cttaggattg
     4201 atgttgcaca aaggctggga gcgaattaat ccaatcaaaa atacttcagg tgtcggatat
     4261 agagcatttg cgatttttct aacattggta ttcatcagtt ttacgcggat ttggttccga
     4321 agcgatagcc tcgatacggt aggcatcatt tttgatcgca tcagtcatca tttcggtggc
     4381 gaattgatag aaaatgtatt gtgggggtat cgcacggtat tatcagtgat tgtagttggc
     4441 tatcttatcc actggattcc ggaaagcaca aaacaacaat accgtgagtg gtttgctacg
     4501 cgctcagaac cggctttggt cgcaatttta ataggttata tcttcttgct ctaccaggct
     4561 gtaagtgccg acatgcagcc cttcatttat tttcaattct aagcacctga atatttatta
     4621 acacaaggtg ggaaaaagct gtccccgaaa aggtgtatct taagtggctc agatataatt
     4681 tcgcaatccg ctatgtccac tttttcgcat ctgcataacc ataccatgtt ctcgctgctc
     4741 gacggcgcca gtgacattgg tcgtttatat aaaaaggctg ccgccgatgg tatgcctgca
     4801 ctcgctatta ctgaccacgg taatatgttc ggggcatttc aatttgttgc cgaagcttat
     4861 aaacataagg gcgaagatgg aaaggtgaaa gtaaaaccta tcgttggttg tgaattttat
     4921 ctagttgcgg acagaacaag gaaacaattt tcaagagaag aaaaggatat ccggtatcat
     4981 caggtattac ttgcaaaaaa tgaaacagga tataaaaacc tggtgaaaat gtgttctctg
     5041 ggatatatgg agggcatgta cggaaaatat ccacgcatcg ataaaacact cattgaaaaa
     5101 tatcacgaag gccttatcgc taccacatgc tgtcttgcag cggaggtgcc cagaacgatt
     5161 ttgaaaaagg gtagggaaga aggggagaaa gtttttaaat ggtggctgga tatctttggc
     5221 gaagattatt atatcgaact gcaaagacat gatattcctg agcaggaaac actcaataat
     5281 attttacttg agcttgccga taaatataat gtaaaagtaa tcgccaccaa tgatgcgcat
     5341 tatgtagatc aacaagatgc taatgcacat gatattttat tatgtatcaa tacaggtgaa
     5401 aagcaggcga caccgaagat gaaggatttc ggtgatgatg atatgatcat taaagggaag
     5461 cgttttgcat tctataatga tcagttttat tttaaaacac aatctgaaat gaacgaattg
     5521 ttccacgatg ttccacaggc actggacaat acaaatgaga ttgtagataa agtagaactg
     5581 ctcgatctta agcgtgatat cttgttgccg gcattcccca ttccggatgc atttaaaatt
     5641 catggtgacg atacaactaa tcagtgggaa tacttgaagc atctaaccat ggaaggtgct
     5701 cataagcgct atggcacaat ggatgcggaa acagaagaac gcataaattt tgaattgttt
     5761 accattaaaa caatgggatt cgcaggttac ttcctgattg tgatggattt cattcgcgca
     5821 ggaagagaac tcggggtttt tgttggcccc ggtcgtggat ctgcagccgg atcagttgtg
     5881 gcatattgta ttggaattac aaatatcgat ccgataaaat ataatttact tttcgaacgc
     5941 ttcctcaatc ccgacaggaa gtcaatgccc gatatcgata cggactttga tgatgatgga
     6001 cgtcaaaaag ttattgatta tgtggtagat aaatatggta aaaatcaggt agcgcaattg
     6061 gttacctacg gcacaatggc agcaaaaact agtatcaaag atgttgcccg ggtaatggat
     6121 ttgccaattg ccgacagtaa tgccttatca aaactcgtac ctgaacgtcc gggtatttct
     6181 cttaaacggc ttattcaagc accaatatca ggtgatggca gtctttcgga aaaagaaaat
     6241 cttactccgg atgaattaaa taatgcaaaa gcattacgcg caattgccga agatcaaaaa
     6301 gatattcgca gtaaaataat taaagaggca ttgatccttg agggtagtgt gaggaataca
     6361 ggcgtacatg cagccggttt gattatcgca ccaaaagatc ttacagattt attgccaatt
     6421 gctgtcgcaa aagactcggc tctttatgtt acacaatttg aaggagaagt aatcgagagc
     6481 gcaggtgtaa tcaagatgga ctttttaggt ttgcgtacgc tgtccattat taaaacagca
     6541 ctaaagctga ttaaagaaaa tcataatgtc attatcgata tcgataccat tccgctagat
     6601 gatctcaaaa ctttcgaatt atatcaacgc ggtgaaacaa acgccacctt ccagtttgaa
     6661 agtccgggaa tgcaaaaata tttacgcgaa ttaaagcccg ataaattcga agacctgatc
     6721 gcgatgaacg ccttatatcg ccctggaccg cttgaatata tccctaccta tattaagcgt
     6781 aaacatggcc gcgaacctat tgaatacgac cttcctcaaa tggaagaata cctcaaggaa
     6841 acctacggtg ttactgttta tcaggaacag gtgatgctgt tgtcgcagaa attggcaggc
     6901 tttactaaag gagatgctga cacgctgcgg aaagcaatgg gtaagaagca aaaggatgta
     6961 ttggataaaa tgaaatccaa gttccttgag ggagctgata aaaataatat tgataaaaaa
     7021 gtatgtgaaa aaatctggac agactgggaa gcatttgcac aatatgcctt taataagtcg
     7081 cactctacat gttatgcatt tgtcgcttat caaacggcct atctgaaagc acattatccg
     7141 gcagaatata tgagtgccgt attaacacat aaccagggga atatcgacaa ggtaagcttc
     7201 tttatggacg agtgtcgccg catgggtgtt cctgttttac cgcctgatgt taatgagagt
     7261 gatctcaatt ttgctgtgaa taaaaacggt cagatacgat tcggtatgtc agctatcaaa
     7321 ggagttggtg agtctgcagt aattgaattg atcaatgaaa gaaaaaagga tggtgcgttt
     7381 aatagcttgt ttgatctgac cagaagggta agcctgcgaa gcgtaaataa aaaaacattt
     7441 gaggctttag tttatgccgg agcatttgat agtttcggat taaatcgctc acagtatttt
     7501 gaactcgcaa aaaatgaaac actgaacctt attgaacaag ccattcgata cggaaacaat
     7561 ttcaatggtg ataccgtaaa atcacaaaat accttatttg gcgactctga ggatgttgaa
     7621 atagcagaac cgaagattcc ggatttccct gagtggaata ccatagagaa attgaaaaag
     7681 gaatttgaag ttgttggcat gtacatgagt ggtcatccgc tcgacgacta taaaattgaa
     7741 gctgacgctt tcaaaacaac taccattacg gatattgaga aattcacgga taagggagac
     7801 attgttttgg taggttatgt ggcttcaaca gatactaaag tgggtaaaac aggagataag
     7861 tataccatat ttacactgga agatttctcc ggacatctgg aaatgggatt atttaagaac
     7921 aattatatca agttcaaaaa ttatgtagat cagaccggaa atgtgttgta tgtgaaagga
     7981 gcttatcaaa ccgaccgaaa cgacccggaa aggaagcgtt tctgggtaag tgacattcag
     8041 ttgctgtctg aggtaaggga gaaaaaatcg atgaaaataa cccttagcat gagcctgtcg
     8101 gacctgaatt cgaacttttt ggaaggtctc tcagaggttt tgaagcagca ccccgggaaa
     8161 gacagcattg tgctcaaggt gatagagcat accgagcagt ttgacctgct tatgcgcaat
     8221 aaacagggcg gtatcagggc ggaaagtgca gttttgaaca gtctggggaa atttggggaa
     8281 gtcaatttaa cagtagtcgg ctaattttgt gtatcccaaa cgggaacttt tacaggtatc
     8341 cgatgttatg cgtaatacct gttcgaaaaa cagctttaaa ccaacttaag aatatggcta
     8401 tagaatttaa cgacgcaaac ttcaagcaaa cagtgcttga cagcggaaaa ttgagtgtga
     8461 tcgacttttg ggcagaatgg tgtggccctt gtcgcgtagt aggtccggtt atcgaagaac
     8521 tggccaaaga ctatgatggc aaagtgaaca tcggaaaagt gaatgtcgat tttaaccctg
     8581 aaatctcgat gatgtacggt atccgtaaca tcccgacgat cctgttcatt aaaaatggtc
     8641 aggtggttga caaacaagtt ggtgcagtgc caaaaagcgt ccttgatgct aaggtgagac
     8701 aacacatgta attaatttat actgaagaaa agcctcctat cgtttgacag gaggcttttt
     8761 acgttttatc ttgcacctta attgctatgg acaggcagct aatatctcta caagatctcc
     8821 atcaggttga aaacctcgaa ctcatcgcca aacaggtggt ggaaggattt atcctgggat
     8881 tgcataaaag tccatttcat ggattttcag ttgaattcgc tgaacataga atttataacc
     8941 tgggagaggc aacacggcat atcgattgga aagtattcgg gcgaacagat aagctgttca
     9001 caaaaaaata tgaagaggaa acaaatctgc gctgtcatat tgtgatcgac gcatcctcca
     9061 gtatgtattt cccggaaccg gataaacagg cgaagggttt gcaccttaat aaactgatat
     9121 tttcagcaat ttgttcttct gccatcatcc atttgctgaa aatgcaacgc gatgcagcag
     9181 gacttacaat atttgcagat gatgtgctct tgcataccaa agcaggaagt agtaatgtgc
     9241 atcacaaaat gctccagacg caattggaaa gttttattgg caaatcttca catgatctgc
     9301 cgacaaatgc cggtaaatgt ttgcaccaaa tcgccgaaaa tattcaccgc cgcagcatgg
     9361 tggtaatttt tagtgatatg atggaagatg ttgaaaaatc tgaagagttg ttttctgcac
     9421 tgcaacattt aaagcataat aaacacgaag tgattctctt tcatgttaca gataagcgac
     9481 atgaaattaa ttttgagtac gataatcgac catacatctt tgtcgatatg gagaccggtg
     9541 aaaaaattaa agtgcgcagt agcgaagtaa aggaaaaata caccgagcaa atggccaagt
     9601 atatgcaaca actgaaattg cgttgcggtc aatatggagt cgacctcatt gaagctgata
     9661 tgatggagga ttttcgtgat gtattgttgc cttatttggt tagaagaggt aagatgcatt
     9721 gaggctataa aggtttcaag attagtatgc ttaaaggtcc gaagattcaa agatttgaag
     9781 attcgaaggg tatagggatt agtaaattag tatgttttag ggtgtgaaga ttcaaagggt
     9841 ttcggggttt gaagctgaga gggtttgatg gtaaaaggtg tgggttttgt ggagtgga
//
LOCUS       DAOQOU010000124         9825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3660_length_9825_cov_4.796622, whole genome shotgun sequence.
ACCESSION   DAOQOU010000124 DAOQOU010000000
VERSION     DAOQOU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_9825_cov_4.796622"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..3249)
                     /gene="dnaE"
                     /locus_tag="PLM90_10565"
     CDS             complement(283..3249)
                     /gene="dnaE"
                     /locus_tag="PLM90_10565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMZ89862.1"
                     /translation="MFLNTHSYYSLRYGVLSPEGLLAEAQRCGVHAFAMTDINNCSGW
                     FELYAEAKKFNIHVVPGVEFKNGDDHLYIGIAKNEHGFAELNRYLSHYLNTKEPIPAI
                     APEFEHAIIIYPFVADKIYVLGDNTFVGIQKKDLSKLRVGKSGLPQEKLVAMLPVTFS
                     SKKDFSAHRILRAIDHNTIITKIPENATALPDQVFRPYDRLYEDFGDHGYLLENAREL
                     LENCTFDFTYHTNKNIRSFSGNEADDINQLLQLTYEGVKRRYGKLTKTIQDRINKELE
                     IIIQKGFVAYFLINWDIIRYAHSKSYFTIGRGSGANSLIAYCMGITDVDPIELDLYFE
                     RFINLFRETPPDFDLDFSWKDREDVTDYIFKRYGEEHTVLLATYTTFKSRSFIREVAK
                     TFGLPKYEIDAMIKNYGESIFGDSIQIPDKIARSIYNFGAYLQGNPNYLGIHAGGILI
                     SELPVYYYTATHLPPKGFPTTQFDMHVAEDIGLYKFDILSQRGLGHIKETVEIVKQNR
                     GDDIDITRVQDFKKDPVLNNNLVKGNTIGCFYIESPAMRSLLNKLLCDNYLTLVAASS
                     IIRPGVAQSGMMDEYIRRHRFPEERKRIHPVMGDIMPDTYGVMVYQEDVIKVAHYFAK
                     LTLSEADVLRRGMSGKSRSKDEFQRIKTKYFENCAQLGYDKALANDVWHQIESFAGYS
                     FAKGHSASYAVESYQSLHLKSYYPLEFMVAVINNFGGYYSTELYFHEARKSGATIEAP
                     CINNSRYNTCIYGSVIYVGFVHLSGLESALAEQMLAERAHNGPFTSFDNFLDRVAINL
                     EQLELLITINAFRFTGITKRELLIEAYTRMNKTKKTIPAATLFEKSAPPKYTFPPLHF
                     DAREDAWDEIKLLGFPLSSPFLLISDPIIENRNLKLVKDLPKFVGKTVEIIGYLITTK
                     RTSTKHGEPMYLGTFIDMEGNWLDTAHFPAVAKQYRFKGKACYLLKGKVQESFQAHTL
                     IVSEMYHLKMWVEGEN"
     gene            complement(3534..4736)
                     /gene="dinB"
                     /locus_tag="PLM90_10570"
     CDS             complement(3534..4736)
                     /gene="dinB"
                     /locus_tag="PLM90_10570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMZ89863.1"
                     /translation="MRLSMDHSGRMIVHIDLDTFFVSVERLKDDRLKGKPVAVGGTGD
                     RGVVASCSYEARAFGVHSAMPMRQARILCPELVTVRGDYEHYSHYSNTITEIVKENVP
                     LYEKSSIDEFYIDMTGMDRFFGTYTYMQELRERIIRETGLPISFGLSENKTVSKVATG
                     QAKPNNHMKIDHGVEKSFLAPLPVKKIPMVGEVTGQLLRKMGIVQIKTIQEMPQQVME
                     NILGENGRVIWRKANGIDTSPVEPYTERKSISSEETFESDTIDVSRIRTILMAMTENL
                     AYQLRKEGKLTSVVAVKIRYSDFDTHTKQIRIPYTSTDHTLMRVVKELFDKLYERRVL
                     IRLVGVRLSGLVNGGYQINLLEDAEHTIRLYQEMDHVRKRFGDTVVQRASAMGYKMRR
                     FNPFKGER"
     gene            5082..5630
                     /locus_tag="PLM90_10575"
     CDS             5082..5630
                     /locus_tag="PLM90_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89864.1"
                     /translation="MKKIVAYIGCITLMATLLINSNGFTLWLSAATYHAKQEVWNRIE
                     AGEVNDIATTLTIPLDHVEHALNGFSWRDDKHEFTYEGFLYDIIEMDMASDGYHIIAV
                     RDDKEKDLEEKIASQLQHQNNTDQKHQSLPKLQLDTQFDVVALTAYFVPDQTLIMQTN
                     VSKFIESSGYLNSQINPPDELI"
     gene            5682..7772
                     /locus_tag="PLM90_10580"
     CDS             5682..7772
                     /locus_tag="PLM90_10580"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ89865.1"
                     /translation="MTVKLIRLLFVCCTVLFARPMQAQQTTTCGTDDVRRQLIEQYPE
                     IIQRERAFNIYAQKFDLSQLKVEEGTYIIPVVFHVIHDYGVENISDEQIFDAMEKINE
                     DFNKENSDLAETVTEFLGIAADVQVEFRLAQRELSGACTNGIDRIPSMRTYTGGDAAK
                     LGGWQHGFYLNIWVVKFLAGSAAAYAYYPTSIFDALAPVDGVICRYDYVGSIGAAGEY
                     SSHTLSHEIGHYLNLQHTWGNTNNPGVECGDDQVDDTPVTKGWLSCNLAGHTCIEPLD
                     NVQNFMEYSYCSTMFTEGQKTRMYAALNSTDAQRNHLWSSDNLMLTGTNDGYVAGLCS
                     PEADFYTSQRFVCKDESVTFHDASWRGDVDSRMWTFEGGSPLTSTEMDPVVSFNTPGW
                     HSVTLQVTNASGTTTKTVEQYIYVSDDVASYNADYVGNFNNMDAALNDWVLYNRYPDN
                     YQWNWRGQNGYWNTPCIWLNSRFGPDQEKDEVISPSFDLSDGLTDNVFFKYSTTSNGF
                     DADDYTMKLKLYYSTNCAKTWISVGEITGDDLITSYGGSSNFFPVFADQWRVAAFSIP
                     SGAKAENVRFKIEFVYNNYVNNVFVDDFNFTDGTLGIEGFGGNGTLAVTPNITGVQDV
                     VSLHYTAETAGIVQVQVSDITGRVITTQQTAVTSTGPQTIQLNLADLHLSAGCYFVTL
                     HAHGANATARLVLQ"
     gene            complement(7855..9084)
                     /locus_tag="PLM90_10585"
     CDS             complement(7855..9084)
                     /locus_tag="PLM90_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMZ89866.1"
                     /translation="MSDPTQKSILTELAAAAGLQTLEKYADVDQKKQRLFIGIPRETG
                     FQENRVPLTPDSVETLVAQGHRIVIETKAGEGARFSDKDYADAGAMVMYDRKQVFEAD
                     IILKVTPPAMDEMELFRHGQVLISPLHLPTLRREIIHDLMKKQVTALAFEYVKDESGT
                     YAFVRCMSEIAGASAILLASELLADAIHGRGYLLGGLSGIPPTQVLILGAGVVGEFAT
                     RAALGLGATVKIYDNNVYKLSRLQNNIGERFYTSVINNRRLLNDIRTADVVVGAIHAE
                     SGRTPVIVTESMVMEMKPGSVIIDVSIDQGGCIETSEVTTHLQPTFIKHDVIHYCVPN
                     IASRVPRTASYAISNIASSTLQQFTEYGGLDPLIRNLEGIRHGVYIYKGHLTNYYLAQ
                     KMEIKYTDINLLLPSVL"
     gene            complement(9132..9557)
                     /gene="tsaE"
                     /locus_tag="PLM90_10590"
     CDS             complement(9132..9557)
                     /gene="tsaE"
                     /locus_tag="PLM90_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HMZ89867.1"
                     /translation="MKPRIFTLDETTQIAKMLNAEAKHSHIFLFHGEMGSGKTTLIKS
                     MCRQLGVNEHTSSPTFAIVNTYQGTQHIIYHFDLFRLRDVHELEAIGFSEYVDSGNML
                     FIEWPEQALPLLKGYEVVKIVLETIDTEHRRISMQIETL"
BASE COUNT         2699 a         2199 c         1982 g         2945 t
ORIGIN      
        1 gactaggaga ctttcggtga gtggtgagtg ggtgagtggg tgcttgcaca cttccacact
       61 tgcacacttg cacacttgca tacttccaca cttgcatact tccacacttg catacttcca
      121 cacttgcata cttgcacact tgcatacttg cacacttgca tacttgcaca cttccacact
      181 tccacacttg cacacttcca cacttccaca cttacattcc tccgcattta tctgctaatc
      241 agctaatcgg ctaattggct aattggctaa ttggctaatc ggctaattct ccccctccac
      301 ccacatcttc aaatgataca tctcagaaac aatcagtgta tgcgcttgaa aactttcttg
      361 cacttttcct tttaggagat agcatgcttt tcctttaaag cgatattgtt ttgctaccgc
      421 cggaaaatgc gcggtatcta accagttgcc ttccatatcg atgaaggtgc caagatacat
      481 cggctcaccg tgttttgttg aagtgcgttt tgtcgtgatg agataaccga tgatctctac
      541 cgttttacca acgaatttcg gcagatcttt taccagtttt aaattgcgat tttcaataat
      601 cggatcgcta attaaaagga agggcgatga cagtggaaat cccaataatt tgatttcatc
      661 ccacgcatct tcacgggcat cgaagtggag tgggggaaag gtgtatttcg gtggcgcact
      721 tttttcgaac aaggtagcgg caggaattgt tttttttgtt ttgttcatgc gcgtatatgc
      781 ttctatcagc aactcgcgtt tggtgatacc cgtgaagcga aaagcattga tggtgattaa
      841 taattccagt tgctcgagat tgatggccac gcgatcgaga aaattgtcga aggaagtaaa
      901 aggcccattg tgcgcacgct cggcaagcat ttgctcagcg agtgccgatt ctagtccgga
      961 taaatgcaca aatcctacat agatcaccga gccgtaaatg caagtgttat accggctgtt
     1021 attgatacac ggtgcttcaa tggtggcgcc actttttcgc gcttcgtgaa aatataattc
     1081 ggtgctgtaa tatccgccga aattattgat tacggcaacc ataaattcta gcggataata
     1141 acttttcaaa tgcaaactct gatagctttc tacggcataa gatgcagagt gtccttttgc
     1201 aaatgaataa ccagcaaaac tctctatttg atgccataca tcgttggcaa gtgctttatc
     1261 ataacctaat tgcgcacagt tctcgaaata ttttgttttg atgcgttgaa attcatcttt
     1321 actcctgctt tttccactca tgccccgacg cagtacatct gcttcactca atgtgagttt
     1381 cgcaaaatag tgcgctactt tgattacatc ttcctgatac accatcacgc catatgtatc
     1441 gggcataata tcgcccataa caggatgtat gcgcttgcgt tcttcaggaa agcgatgcct
     1501 gcggatatat tcatccatca tgcccgattg cgccacaccc ggacgaataa tggaactcgc
     1561 cgcaaccaag gtgagataat tatcgcacaa caatttattg agtaaactgc gcatggcagg
     1621 cgattcgata taaaagcatc ctatcgtatt tcccttcacc agattattat tcagcaccgg
     1681 atcttttttg aaatcctgca cgcgtgtaat gtcgatgtca tcaccgcgat tttgttttac
     1741 aatttctacg gtttctttga tatgtcccag tccgcgctgg ctcaaaatat cgaatttata
     1801 taagccgata tcttccgcca catgcatgtc gaattgtgtg gtaggaaatc ctttgggggg
     1861 aagatgtgtt gcggtataat aatacaccgg caactccgat atcaagatac caccggcatg
     1921 gatacccagg tagtttggat tcccttgcaa ataagcaccg aaattataaa tgctgcgtgc
     1981 aattttatcg ggaatctgta tgctgtcgcc aaaaatactt tcaccataat ttttaatcat
     2041 cgcatcgatc tcatatttcg gcagtccgaa tgtttttgct acctcccgga taaacgagcg
     2101 acttttaaaa gtggtatatg ttgccagcaa aacggtatgc tcttcgccat agcgtttgaa
     2161 gatataatcc gtaacatctt cacgatcttt ccacgaaaaa tccaggtcga aatcaggggg
     2221 tgtttcgcga aataaattga tgaagcgttc gaaatacaaa tccagttcta tcggatctac
     2281 atcggtaata cccatgcaat aagcaatcag actgttggca ccactgccgc gaccaattgt
     2341 aaaatacgat ttgctatgtg cgtagcggat aatatcccaa ttgataagaa aataggcaac
     2401 gaatcctttt tgtataatga tctccagttc tttattgatg cgatcttgga tagtcttggt
     2461 gagtttgccg tatcgccgtt tcactccttc gtaggtgagt tgcaacaatt gattgatatc
     2521 atcagcctca ttgccgctga acgaccggat atttttattg gtatgatagg tgaaatcaaa
     2581 agtgcaattt tccagcagct ctcttgcatt ttcgagcaga tagccatgat cgccaaaatc
     2641 ttcatacaat ctgtcgtacg gcctgaacac ctgatcgggt agggcagtgg cgttttccgg
     2701 aattttggtg atgatggtat tgtgatctat cgcgcgcaat atgcgatgtg cactaaaatc
     2761 ttttttcgac gaaaaggtaa caggcagcat cgctaccagt ttttcctgtg gcaaccccga
     2821 tttgccgaca cgcagtttgc tgaggtcttt tttctgtatg ccgacaaatg tgttatcacc
     2881 caaaacataa atcttatcgg caacgaatgg ataaataatt atggcatgtt caaattcagg
     2941 agctatggca ggaataggct ctttagtatt cagatagtgc gataaatatc tgttcagttc
     3001 tgcaaagcca tgttcatttt ttgcaatccc gatatacaga tgatcatcac catttttaaa
     3061 ttcaactccc ggcaccacat gtatattaaa tttcttcgcc tccgcataca attcaaacca
     3121 tcccgaacaa ttattgatat ccgtcatcgc aaacgcatgc accccacagc gctgcgcttc
     3181 cgccaacaat ccctccggcg acaaaacccc gtagcgtaag ctgtaatatg agtgggtgtt
     3241 gagaaacatt tgagtgtgga agtgtgaaag tgtgcaagtg tggaagtgta aattgtgttc
     3301 atgtgctcat gtgttcatgt gttcatgtgt tcatgttgaa gtgcactaaa aaacctgcat
     3361 tccgctgcac acttccacac tagtttgtgt tcatgtgctc atgtgttcgt gttgaagtgc
     3421 actgaaaaac ctgcattccg ctgcacactt ccacactagt ttgtgttcat gtttagtcac
     3481 actatgaaac ctgcattccg ctgcacactt tcacacctcc acacttccac actctatctc
     3541 tctcctttaa acggattaaa tcttctcatt ttatatccca tcgctgatgc gcgctggact
     3601 actgtgtcgc cgaagcgttt gcggacatgg tccatttcct ggtagaggcg aatggtatgt
     3661 tcggcatctt ccagtaagtt gatctgataa ccgccattca ccaatcccga taaacgaaca
     3721 cccacgaggc gtatcaatac acgtcgctca tacagcttat caaataattc cttcaccacg
     3781 cgcatcaaag tgtgatctgt tgatgtatat ggtatccgta tttgtttggt atgcgtatcg
     3841 aaatcggagt acctgatttt tactgccacc accgatgtga gctttccttc ttttcgtaac
     3901 tgatacgcca gattttctgt catcgccatc aatatggtgc gaatgcgcga aacatctatc
     3961 gtgtcgcttt caaaagtctc ttcactcgaa atagatttgc gctcggtgta gggttctacc
     4021 ggactggtat cgataccatt tgccttgcgc cagatcacgc gaccgttttc gcccaaaata
     4081 ttttccatca cctgctgcgg catctcttgt atggttttga tctgcactat ccccatcttg
     4141 cgcaacaact gtcccgttac ttctcccacc atcggaattt tcttcaccgg cagcggtgcc
     4201 aaaaaagatt tctctacgcc atgatcaatc ttcatgtgat tattgggctt ggcctgcccc
     4261 gtggctacct tcgataccgt tttgttctcc gataagccaa atgatatcgg caatcccgtt
     4321 tccctgataa tgcgctcccg caattcctgc atgtaagtat aggtcccgaa aaaacgatcc
     4381 atccccgtca tgtcgatata aaactcatcg atcgacgact tctcatataa tggcacattc
     4441 tccttcacaa tctcagtgat cgtattagag taatgcgaat aatgttcata atcaccacgc
     4501 actgtcacca actccggaca caatatccgc gcctgacgca tgggcatggc cgaatgcacc
     4561 ccgaaagccc gcgcctcata actgcacgac gccaccacac cgcgatcacc cgtgccgccc
     4621 acagctacag gcttgccctt caacctgtca tccttcaacc gctctaccga cacaaaaaag
     4681 gtgtccaggt caatgtgcac aatcatccgc ccgctatgat ccatcgataa tcgcataatc
     4741 agtgtcaaat atactgacta tttgctgaca aaaacaaata atattgtcaa aaaaaatatc
     4801 ctcatcccgg aagttcttat cgcacaaaat gaatgaaatg taatacagta ttaaaataca
     4861 tcgaattccc ggctgccgaa ataattgaat taggaatgtc aaaaagtcaa aatgaatgac
     4921 atttggcaca taaattgcct gaatattggt gaagacgtga ataacgggct tctttttctg
     4981 cgtgtacgct actgtataca atcagtaagc aagcgttttc aaaatgacat ggaattgatg
     5041 atgcttatcg cttgaatgtg tatttttacc catctcaggc agtgaaaaaa attgtcgcat
     5101 atatcggatg cattacgttg atggcaacat tgctcatcaa tagtaatgga tttacgctgt
     5161 ggctaagcgc tgcaacatat catgcgaaac aagaagtatg gaatcgaata gaagccggtg
     5221 aagtgaatga catcgctaca accctcacaa tcccccttga tcatgtagag cacgcattaa
     5281 atggattctc ctggcgcgat gataaacacg aatttactta cgagggtttt ttatacgata
     5341 tcattgaaat ggacatggca tctgacggct accatataat tgccgtaaga gatgacaagg
     5401 aaaaagacct ggaagaaaaa atagcgagcc aattgcaaca tcaaaacaac actgatcaaa
     5461 aacatcagtc gctgcccaag ttgcaactcg atactcaatt tgatgtggtt gcattaactg
     5521 catatttcgt acctgatcaa acattgatca tgcaaacgaa tgtatcaaag tttatagaat
     5581 cttcaggcta tctcaatagc caaatcaatc ctccggatga gctgatctga caattgacca
     5641 ttgttttttt ttcagtttta ttcttcttaa atcaatttat tatgacagtt aaacttatac
     5701 gtttactatt tgtatgctgt acagtcctct ttgcaaggcc aatgcaagcg caacagacga
     5761 ctacttgtgg gacagatgat gtacgcagac aattaattga acaatatccc gaaatcattc
     5821 aacgcgaacg tgcatttaat atctatgcac agaaattcga tctttcgcaa ttaaaggttg
     5881 aagagggaac ttatatcata cctgtggtat ttcatgtgat ccatgattat ggtgtagaaa
     5941 atatcagtga cgaacagata tttgatgcca tggaaaaaat aaatgaggac ttcaataaag
     6001 agaacagcga ccttgctgaa accgttacgg aatttttggg tattgctgca gatgtgcagg
     6061 tagaattccg tctcgcacaa cgcgaacttt ccggagcatg taccaatggc attgaccgca
     6121 ttccttccat gcgcacttac acaggtggtg atgctgctaa actcggcgga tggcaacatg
     6181 gattttattt gaatatctgg gtggtgaaat tcctggccgg ttctgctgca gcgtatgcct
     6241 attatccgac ttctatattc gacgccttag cacctgtaga tggtgtaatt tgtcgctatg
     6301 attatgttgg aagtatcggt gccgcaggtg aatattcttc acatacttta tcgcatgaaa
     6361 ttggtcatta tctcaatctg caacacacct ggggaaatac aaataatccg ggtgtagaat
     6421 gtggcgatga tcaggttgat gatacgccgg ttactaaagg ttggctttca tgtaatcttg
     6481 ccggacatac ttgtatcgaa ccactcgata acgtgcagaa ttttatggaa tattcatact
     6541 gttctaccat gtttacggaa ggtcagaaaa cgcgaatgta tgccgcgtta aacagcaccg
     6601 atgctcaacg caaccatttg tggtcatcag ataatctcat gcttacggga acaaacgatg
     6661 gatatgttgc aggattgtgt tctcctgaag ctgattttta cacctcacaa cgatttgttt
     6721 gtaaggatga aagcgttact tttcatgatg catcatggcg tggtgatgtt gattctcgta
     6781 tgtggacttt tgagggaggc tctccactga catcaacaga aatggatcct gtagtttctt
     6841 ttaatactcc gggttggcac agtgttacac tgcaggttac caatgcaagt ggtacaacaa
     6901 caaaaactgt tgagcaatat atttatgtat cagatgatgt agcatcgtat aatgccgatt
     6961 atgttggcaa ttttaataat atggatgctg ctttaaatga ttgggtattg tataacagat
     7021 atcccgacaa ttaccagtgg aactggcgcg gacaaaatgg ttattggaat acaccttgta
     7081 tctggttgaa tagtcgcttc ggtcctgacc aggaaaaaga tgaagttatt tctccttcct
     7141 ttgatttatc tgacgggtta accgacaatg tgtttttcaa atatagcacc acttcaaatg
     7201 gttttgatgc ggatgattat accatgaaac tcaaattgta ttacagcacg aattgtgcaa
     7261 aaacctggat aagcgttggc gaaataaccg gtgatgatct gatcacttct tatggcggca
     7321 gcagtaattt tttccctgtg tttgccgatc aatggcgcgt tgctgcattc tctattcctt
     7381 caggtgcaaa agctgaaaat gtgcgtttca aaattgaatt cgtttacaat aattatgtaa
     7441 ataacgtatt tgttgatgac tttaatttca ctgatggcac ccttggtatc gaaggatttg
     7501 gcggtaacgg cacattggct gtaacaccta atatcacagg ggttcaggat gttgtaagct
     7561 tgcattatac tgcagaaact gcgggtattg tgcaggtgca agtttctgat attaccggac
     7621 gcgtaatcac aacccaacaa acagcagtga cttcaacagg tccacaaaca attcaattaa
     7681 acctggctga tttgcattta tcggccggtt gctactttgt tacacttcat gcacatggtg
     7741 caaacgcaac tgcgcgactg gtgttacaat aataaatttt taagtttggt tttgttcagg
     7801 tcggtgccta tggtatcggc ctgatttttt ttatggtgat gtcaaccgaa gaaattataa
     7861 tacactcggc aataataaat tgatatcggt atatttaatt tccatttttt gtgcgagata
     7921 ataatttgtg agatgtcctt tataaatata aacaccatgg cgtatgcctt ccagatttct
     7981 gatgagcggg tctaatccac catactcggt aaattgctgt aaggtgctgc tggcgatatt
     8041 gctgatggca taactggcag ttcgtggaac acgtgaagcg atattcggca cacaataatg
     8101 tatcacatcg tgtttaataa aagtaggttg aagatgtgta gtcacttcac tcgtttctat
     8161 acatccacct tgatctatac ttacatcgat gatcacactg ccgggtttca tttccattac
     8221 catactttca gttacgataa cgggtgtgcg accactttca gcatgtatgg cgccaaccac
     8281 tacatctgcg gtgcggatat catttaataa tcgtctgtta ttaattacag aagtataaaa
     8341 acgttcgccg atattgtttt gcagtcgcga taatttatat acgttgttgt cataaatttt
     8401 caccgtagca cccaatccca aggcggcgcg tgttgcaaat tcgccaacaa caccggcgcc
     8461 aagtattaat acctgagtag gtggaatgcc cgatagtccg cctaataaat atcctcttcc
     8521 atgaatagca tcggctaata attcagatgc tagtaaaata gcgcttgcac cggcgatttc
     8581 gctcatacat ctgacaaatg cgtacgtgcc gctttcatct tttacatatt cgaatgcgag
     8641 tgcagtaacc tgttttttca tcaggtcgtg aataatttct ctacgcaggg tagggagatg
     8701 taatggcgat atgagcacct gaccatgtcg gaacaattcc atttcatcca tggcaggagg
     8761 cgtaactttt aatatgatat ccgcttcaaa aacctgcttg cgatcgtaca tcaccatcgc
     8821 accggcatcg gcataatctt tatcgctgaa ccgcgcgcct tcaccggctt ttgtttcaat
     8881 cacaatgcga tgaccttgcg caaccaatgt ctccacacta tcaggagtca gcggcaccct
     8941 gttttcctga aagcctgtct cacgaggaat tccgataaac aatcgctgtt ttttttggtc
     9001 aacatcggca tacttctcca atgtctgtaa tccggcagcc gctgccaatt cggtaagtat
     9061 agatttctgg gtcgggtcgc tcatgctata aaattaagca acgggcatca tgtagcaaaa
     9121 tgcctgaaag ttcacaatgt ttctatctgc atggaaatcc tgcgatgctc agtgtcgata
     9181 gtttccagca ctatcttcac tacctcatat cccttcagca ggggcaaggc ttgttccggc
     9241 cattcaataa acagcatatt ccccgaatct acatattccg aaaatcctat ggcctccaat
     9301 tcatgcacat cgcgcagcct gaaaaggtcg aagtgatata tgatatgctg cgttccctga
     9361 taagtattta cgatcgcaaa agtgggggag gaggtatgct catttacgcc aagttgcctg
     9421 cacatcgatt ttatcaatgt tgtttttccg gaacccattt cgccgtggaa aagaaaaatg
     9481 tgagaatgct ttgcctctgc attaagcatt ttagctattt gggttgtttc gtccagcgta
     9541 aaaattcttg gcttcataac ttcatacaaa aatgcttaat aactagaatc tctgtgaatt
     9601 tattttattt ttatttgcat gtaaatctta caactataat aattcttact ttggtttaaa
     9661 gcataaattg taacggcatt tcttcaatta gtcttttgtg atcgaacctt gaggtaggca
     9721 tccccattca ctttttctat attccgtttt gtgagtttga tcaaatccaa tgcttagggc
     9781 gcctccgtct cgtttaagac gcttgccgga ctttgatgct tgcag
//
LOCUS       DAOQOU010000125         9767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3695_length_9767_cov_4.978274, whole genome shotgun sequence.
ACCESSION   DAOQOU010000125 DAOQOU010000000
VERSION     DAOQOU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_9767_cov_4.978274"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLM90_10595"
     CDS             <1..946
                     /locus_tag="PLM90_10595"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMZ89868.1"
                     /translation="NANLQGLADLFYEGNVVLINNVGYPDPNRSHFRSMDIWQTASDE
                     NVYMQTGWIGRMLDQSCKSGDCQKPHFAVEIDDSLSLALKGESMSGFAVRNPDQLKNV
                     NLNPFIDSVARSGGDIHTHDNVGYLHKLLTDTTESTDYIYDQSKKYTTAFTYPTSEFA
                     AQLKIIANLINAETNTSVYYVSLSGFDTHALQRGQQERQLRIYSEALQAFTKDLKSTN
                     RFKDTLIMTFSEFGRRVEENAGKGTDHGTANNVYLIGGGMKQGGMINDMPDLSKLVDD
                     DLVYQTDFRNIYATILDKWLEVPSKEILGRDFARMDFL"
     gene            complement(1511..3052)
                     /locus_tag="PLM90_10600"
     CDS             complement(1511..3052)
                     /locus_tag="PLM90_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89869.1"
                     /translation="MKNLIEISKIVTKKRISKIEIFDKSLLNKRDSKFNEFYEGLVND
                     RFTTDEEAASILYETSPLDDKYRQLKSRFSKRLLNTLFFLDSNDPSFSDYHRANYSCN
                     KNWAQIRILLSSGARSSAIKIATKTLSVAQTYRITDMILNCARILMSNASLSGDDNEY
                     DYYADLTHKALRDMGFEIRSEELYQQLSINFAAAGYSDPELKALADKSLDEVNKLIKQ
                     TDSYQLHYMRYQIWIIGTQIKGQFKETIRVCEEAENYLESNPNFNQKIKLANLALIKI
                     SAYLQLRDYDNGKRNAEKCLELVQEGSNNWFVFLEYYFLLAMHTENYINAAAIFIKVI
                     NHPRFMYTGDERKEKWKIFEAYINYIFETENLDRDLLATDATKRKFRLLKFLNEVPNF
                     SKDKRGYNVSILVIQILYMLEKGEYSGIINRSEALNVYCSRYLRKDDAYRSSCFLKML
                     LTMVKEDFVFDRTKQMAQKYLRKMMAGDPSGNNIVNEWEIIPYEILWEKVLNRLKNKT
                     VAKQV"
     gene            complement(3666..4901)
                     /gene="clpX"
                     /locus_tag="PLM90_10605"
     CDS             complement(3666..4901)
                     /gene="clpX"
                     /locus_tag="PLM90_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HMZ89870.1"
                     /translation="MKKKDSAYCSFCLRPKEDVRILIAGQEGHICENCVAQAQQMVDE
                     ELMQPVKRYKFALTNSMKPRSIKEFLDTYVIGQDEAKKILSVAVYNHYKRLNQKPLED
                     VEIEKSNIMVVGRTGTGKTLLARSIAKFLNVPFTIVDATVFTEAGYVGEDVESILSRL
                     LQVCNYDVSAAEKGIVYIDEIDKIARKGDNPSITRDVSGEGVQQALLKMLEGTEVMVP
                     PQGGRKHPEQKMVKINTQNILFICGGAFDGIEKIIARRTATQLIGFKRNEDETAVDRE
                     NLLQYIAPQDLRAFGLIPELIGRLPVLTHLDPLDATTLRAILITPKNAIIKQYEQLFL
                     LEGIELKVDDAVLDFIVDKAIEFKLGARGLRSICEALLTDAMFDLPSSPDAKVFHLTL
                     EVAQQKFNQSKLSLLKIAS"
     gene            complement(4949..5638)
                     /gene="clpP"
                     /locus_tag="PLM90_10610"
     CDS             complement(4949..5638)
                     /gene="clpP"
                     /locus_tag="PLM90_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HMZ89871.1"
                     /translation="MHPNEFRQYAIKHLGMSSNALDSYMNVSSMHVTNLTPYIIEERQ
                     LNAQALDVFSRLMMDRIIFLGTGIDDYVANVVQAQLLFLSSVDAKRDIQIYINSPGGV
                     VYSGLAIYDTMHYVTPDVATICTGIAASMAAVLLCAGTKGKRSALGHSRVMIHQPMGG
                     AQGQASDMEISLKEILKLKQELYNIISSHSGQPFDKVEKDSDRDYWMRAEEARDYGMI
                     DEVLVRDPKKS"
     gene            complement(5628..7007)
                     /gene="tig"
                     /locus_tag="PLM90_10615"
     CDS             complement(5628..7007)
                     /gene="tig"
                     /locus_tag="PLM90_10615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HMZ89872.1"
                     /translation="MNVNVENLGSQHHIVHITLDHADYKEPFEKALKEIGKKAQVPGF
                     RPGHVPAGMIRKMYGESVILDELYKLVNEQMNNWLKTNNHELLGDALPVNKDLGVDYN
                     SNKSYEFSYEVGVQPEVEVESVLRNAAAFTRYRIPATEAEIDQEVERMQRKYGERKDV
                     ESIEENDVVYAHAHQLNDDGSEQEGGMHVDTYFNLQMLNDDQKDVFLGAKSGETKNIM
                     DIFSVFKGDKVRVAKNVLQMGEVTEAQLEDFNPAFSFRIDRIARLFDAEINEAFFTEV
                     SKEVGPVTNMDELRNQIRGAIELYNDQMTDVNLENNIFTYLMDTTNVPLPDVFLRKWY
                     VQANRKSEEAEDFEKEFTEFITRLKQSLIYRKVQQTHQIEVSNEEIVQEATATVARSY
                     AQLGDDFIQYVLGSQLKDKQFVENMHDRVAQKKFFAALRGYVAVEEQSITLEAFQEMN
                     KKTEETYAS"
     gene            complement(7085..7169)
                     /locus_tag="PLM90_10620"
     tRNA            complement(7085..7169)
                     /locus_tag="PLM90_10620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7133..7135),aa:Leu,seq:tag)
     gene            complement(7379..9304)
                     /locus_tag="PLM90_10625"
     CDS             complement(7379..9304)
                     /locus_tag="PLM90_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ89873.1"
                     /translation="MNYLQAEGLSKTYDTKVLFDNISFTINRGQKVALVARNGAGKTT
                     LLRVLTGKDFADAGKVSVHKSITLGILEQDPMLDESLSIIDNVLSGDSPSIRAIRAYE
                     EALMAQEHHFNEETSTALERAHDAMHALNAWDYELRIKQILSHLNITELDQKVSTLSG
                     GQKKRIALSKLLIEEPDMMILDEPTNHLDIEMVEWLEEYLTSRNITLLLVTHDRYFLD
                     RVCNEIIELENGKVFSYRGNYSLYVEKKAHRDMVEAAELEKVRNTYRRELEWVRRMPK
                     ARTTKSKSRLDAFDEIAEKAKGKQKQEALTLDVKMTRMGGKILEMIKVSKAFGTLKIV
                     EQFTYLFKQYDKIGIIGKNGMGKTTFLNLIMELEKPDAGKITTGETVVYGYYSQSGMV
                     LKEDKRVIEVVKDIAEFIPLADGSKLSAAQLLTKFQFPPETQYTFVSKLSGGEKRRLY
                     LMTILIKNPNFLILDEPTNDLDILTLSILEEFLADFKGCLLIVSHDRYFMDRLVDHVF
                     VFEGNGVISDFPGNYTEWRDAQDELQRKQKQGPDPVAESSTSTITKQSERSSIQEPVV
                     VTAKRKPTFNEKREFELLSKELPELEIQKLQLEARLIEVAADHVKLLEVTTELAAINE
                     ALDEKGLRWLELSEIVG"
BASE COUNT         2510 a         2217 c         2102 g         2938 t
ORIGIN      
        1 gaatgcaaat cttcaaggct tagctgatct gttctacgaa gggaatgtgg tactgattaa
       61 taatgttgga tatcccgatc ctaaccgctc gcatttcaga tcgatggata tctggcaaac
      121 tgcaagtgat gaaaatgtgt atatgcaaac agggtggata ggcaggatgt tagatcaatc
      181 atgtaaaagt ggagactgcc aaaagccgca ttttgctgtt gagatcgacg acagcctgag
      241 ccttgcatta aaaggtgaat cgatgagtgg ttttgctgtt cgtaatccgg accaattgaa
      301 gaatgtgaat ttaaatccgt tcattgatag tgttgcccga agcggcggcg atatacatac
      361 acatgataat gttggttatt tacataaatt gctgacagac actactgaaa gcacggatta
      421 tatttatgat cagtcgaaaa aatatacaac agcgtttact tatcctacat cggaatttgc
      481 agcacagtta aaaattattg ctaacctgat taatgcggaa acaaacactt ctgtgtatta
      541 tgtatcgtta agtggattcg atacacatgc gctgcaacgt ggacaacagg aacgtcagtt
      601 aaggatttat tcggaggcct tgcaggcgtt tacgaaagat ttaaaatcta caaatagatt
      661 taaggataca ctgatcatga cgttttctga atttggcaga agggtggaag aaaatgccgg
      721 taaaggaacg gatcatggta ctgcgaataa tgtatattta attggaggtg gaatgaagca
      781 gggcggcatg ataaatgata tgcctgattt atcgaagttg gtagatgatg atttagttta
      841 tcaaacagat ttcaggaata tttatgctac gattcttgat aaatggcttg aggtgccgtc
      901 caaagaaatt ctcggacgag actttgccag gatggacttt ttgtgaccaa tactgagtaa
      961 agcattcggt ttttattaac atgagcctct tgatcggttt cgggatgaag gtttggaaat
     1021 ttttgccttt taagtatttc taagcgaggt gtgctaagcg cttgtattcg agtagaagcg
     1081 gttgctcgcg ttagattcaa tgtctgcctg acattccaga tttaagacct aacaggtttg
     1141 agacctgtta ggtttaaaac ctgttaggtt taaaacgtgt taggtttaag acctgttagg
     1201 tttaagacaa tgggatgatt caattacccc cccccattca acctccataa attatcaaaa
     1261 gaaaatattt cgattcgggc aactgcaccc tcattctcac catgcatctt ctaaacacac
     1321 cactcgctac tcaagagtca cggcattagt atgtagaggc aagtatctag aatttgtatc
     1381 aggtatcagg tatatagtat caagtatcaa gtatcaagta tcaggtattt gtatactcgc
     1441 atcacaaacc caatacttaa gcacgcacca cactcaccac tcaccactca ccaccctcac
     1501 cactcaccac tcacacttgc ttcgctaccg tcttattctt caggcgattg agcacttttt
     1561 cccataaaat ctcgtaagga attatttccc attcattcac gatattgttt ccactcggat
     1621 cgccggccat cattttgcgt aaatatttct gggccatttg tttggtgcgg tcgaatacaa
     1681 aatcttcctt caccatggtg agcaacattt tcaggaagca gctgctgcga taagcatcgt
     1741 cttttcgcag gtagcgactg caataaacat ttagcgcttc agagcggttg atgataccgg
     1801 agtattctcc tttttcgagc atgtataaaa tctgaatcac gaggatggat acattgtatc
     1861 cgcgtttatc cttgctgaag tttggtacct cattgaggaa tttcaacaag cggaatttgc
     1921 gtttcgtagc atccgttgcc aacaggtcgc gatcgagatt ttctgtttcg aagatgtagt
     1981 tgatataagc ttcgaatatt ttccatttct ccttgcgctc atctcctgtg tacatgaagc
     2041 gcgggtgatt gattacttta atgaaaatgg ctgctgcgtt gatatagttc tcggtatgca
     2101 tggccagcag gaaataatat tcgaggaaaa caaaccagtt attacttcct tcctgcacca
     2161 attcaagaca cttttctgca ttgcgttttc cattgtcgta atcacgcaat tgcagatatg
     2221 cacttatttt gatgagtgcg agattggcga gttttatctt ttgattgaag ttgggattac
     2281 tctccagata attttctgcc tcttcacata cgcgaatagt ttccttaaac tgccctttaa
     2341 tttgtgtacc tatgatccaa atctgatagc gcatataatg caattgatag ctatcagttt
     2401 gtttaatgag tttgtttact tcatcaagcg atttatcggc aagcgctttt agttccggat
     2461 cactgtaacc ggcggcggcg aaattgatac tcagctgttg atataattcc tccgaacgta
     2521 tctcaaagcc catgtcgcgc aatgccttgt gggtgaggtc ggcataataa tcgtattcat
     2581 tatcatctcc actaaggctg gcgttgctca tcaggatgcg cgcacaattg aggatcatat
     2641 ctgtgatcct gtaggtttgc gctacactga gtgttttggt ggcaattttt attgccgagc
     2701 tgcgggcgcc actcgaaagc agtatcctga tctgtgccca gttcttatta caggaatagt
     2761 ttgcacggtg ataatccgaa aatgacggat cgttgctgtc gagaaaaaac aatgtattca
     2821 acaaacgctt agagaagcgc gactttaact gacgatactt gtcatctaat ggcgaggttt
     2881 catacagtat cgaagccgcc tcttcatcgg tggtaaatct gtcgttgacc aacccttcgt
     2941 aaaattcatt gaacttgctg tcgcgcttat tcaacagact tttatcgaag atctcaattt
     3001 tagagatccg tttctttgtc acaatcttgg agatctcaat caggtttttc atcgggttgt
     3061 ccgggttatc ttgtgtgtga taattggtta aatccttggt tttgttgagt ttcgtgcgaa
     3121 tggctccaaa cattcatgtc acaaattacc ctacaatacc acaaacaaat atgaatattt
     3181 aaccgcatat tcgcaacaag ttatccacca tctatgccga tttacgcggt tatgggacag
     3241 tattccgatt gcaaaaatgt cacaaaaaaa taataaattg taattttaaa accgataaat
     3301 aaggcattta gcgcgaataa tcggttataa ccgcttaatt aatcatgtca caaaatgtca
     3361 tttgctgaaa atgatttacc cattcatctg gttacttttg atttgtttat ttgacagcct
     3421 tttaaataat acagcgtatg acaactttta cactaccgaa ttttcccgtt caggccatcg
     3481 tgatcgtaga cctggacgag ctatagaaag acagttggtt tgtttcataa attaggttca
     3541 cgaccgaaag gaggcgcaag cctccttttg tgttttatgg cctgtcacaa atgatttacc
     3601 tactccataa aacaacaata gcctcatcaa gaggctatcg cgtatatttt gttgctcgtc
     3661 gtttatcagg aggcaatttt caataaactg agcttcgact ggttgaactt ttgctgcgcc
     3721 acttctaatg tcagatgaaa taccttggca tcgggtgatg acggcaaatc aaacatcgcg
     3781 tctgtcagca aggcctcgca aatactgcgc agtccgcggg cgccgagttt gaactcaata
     3841 gccttatcta caataaaatc taaaacggca tcgtcaacct tcaattcaat gccttcgagc
     3901 aggaacaatt gttcatattg cttgatgatg gcatttttcg gggtgatgag aatggcgcgc
     3961 agggtagtag catccagtgg atcgaggtgg gttaaaaccg gcagtcggcc tattaattcc
     4021 ggaatcaatc cgaatgctct caagtcctga ggagcaatat attgtaacag attttcacgg
     4081 tctacagccg tttcatcctc attgcgcttg aaccctatca attgggttgc cgtgcgacgt
     4141 gcaatgatct tttcaatacc atcaaaagcg cctccgcaaa tgaacaggat gttctgggta
     4201 ttgatcttta ccatcttctg ctcagggtgc ttacggccgc cttgaggcgg aaccatcact
     4261 tcagtccctt caagcatctt taacaatgcc tgctgaactc cttcgcccga aacatcgcgt
     4321 gtaatacttg gattgtctcc tttgcgcgct attttatcga tctcgtcgat atatacaata
     4381 cctttttctg ccgccgaaac gtcgtagttg caaacctgta ataacctgct caggatactc
     4441 tctacatctt cacccacata tccggcttcg gtaaacaccg tcgcatcaac aatggtaaaa
     4501 ggcacattca ggaatttggc aatagatcgc gccagcaaag tctttcctgt acctgtgcga
     4561 ccaaccacca taatgttcga tttctcaatc tccacatctt caagcggctt ttgattcagt
     4621 cgcttatagt ggttgtaaac tgccaccgag agtatctttt tcgcctcgtc ctggccaatt
     4681 acataggtat ccagaaactc tttaatagag cgaggtttca tcgaattggt caaagcgaat
     4741 ttatagcgct ttaccggttg catcagctct tcatctacca tttgctgtgc ctgtgctaca
     4801 cagttttcgc aaatatgtcc ctcttgtccg gcgatgagaa tgcgcacatc ctccttcggg
     4861 cgaagacaaa acgagcagta agcggagtct ttctttttca ttccgtaagg cgttatagat
     4921 caaaagtacc ctgtttcagg gaattttatc agcttttttt cgggtcgcga accaaaactt
     4981 cgtcgatcat accgtaatca cgagcctctt cggcgcgcat ccagtagtcg cgatcggagt
     5041 ctttttccac cttatcgaat ggttgtccgc tgtgcgagct gatgatatta tacagttcct
     5101 gcttgagttt caggatttct ttcaatgaaa tctccatatc gctggcctga ccttgtgcac
     5161 cacccatagg ctggtggatc atcacgcgtg agtgacccaa agctgagcgc ttgccctttg
     5221 ttccggcaca aagcaatact gcggccatag aggcggcgat accggtacaa atagttgcta
     5281 catcgggtgt aacatagtgc atggtatcat aaattgccaa tcccgaatac acaacaccac
     5341 cgggactgtt gatatagatt tgaatatcgc gctttgcatc aacactcgac aaaaagagga
     5401 gctgtgcctg cacaacattg gctacataat catcgatacc tgttcccagg aagatgatac
     5461 ggtccatcat caaacgtgaa aacacgtcga gagcctgcgc attcagctgg cgctcttcaa
     5521 tgatgtaagg agtcaaattg gtaacatgca tgctgctgac attcatatag ctgtcgagcg
     5581 cattgctgct cataccaaga tgtttaatcg cgtactgacg gaattcgtta ggatgcatat
     5641 gtttcttcag tttttttgtt catttcctgg aatgcttcca gcgttattga ttgttcttca
     5701 actgctacat aaccacgcaa tgctgcaaaa aatttctttt gagccacgcg gtcgtgcata
     5761 ttttcaacaa actgcttgtc cttgagctgc gatcccaaca catactggat gaaatcatcg
     5821 cccaactgag catagctgcg ggcaacggta gctgtggctt cttgcacaat ctcctcattg
     5881 ctcacttcga tctgatgtgt ctgctgcacc ttgcggtaaa tcagcgactg tttcaggcgg
     5941 gtgatgaatt cagtgaattc tttttcaaag tcttctgctt cctcactttt tctgttggcc
     6001 tgcacgtacc atttcctaag gaagacatcg ggaagtggca cattggttgt atccatcaga
     6061 taagtgaaga tattattttc gaggttgaca tcagtcattt ggtcgttata caattcgatg
     6121 gctccgcgaa tttgattgcg cagttcatcc atattggtaa ccggacctac ctctttagat
     6181 acttctgtaa agaacgcttc attgatctca gcatcgaata aacgtgcaat gcggtcgatg
     6241 cggaaactaa aggcaggatt gaaatcttca agctgcgcct cagtcacttc acccatctgc
     6301 agcacatttt tcgctacacg cactttgtcg cctttgaaga cggagaagat atccataata
     6361 tttttcgtct ctccactttt tgctcccagg aagacatctt tctgatcgtc gttgagcatt
     6421 tgcagattga aataggtatc tacatgcatg ccaccttcct gttcactgcc atcgtcgttg
     6481 agttgatgtg catgtgcata tactacatca ttttcttcaa tgctttccac atccttgcgt
     6541 tcgccatatt tgcgttgcat gcgttctacc tcctgatcga tctcagcttc cgtagccgga
     6601 atgcggtagc gggtaaaggc tgcggcattt cgcaatacgc tttctacctc aacctcaggt
     6661 tgtacaccaa cttcatagct gaactcgtat gatttattac tgttgtagtc gacacccaaa
     6721 tctttattta caggcaacgc atcgccaagt aattcatgat tatttgtttt gagccaattg
     6781 ttcatttgct cgttcactaa cttgtaaagt tcatccagta ttacagattc accgtacatt
     6841 ttacgaatca ttcctgcagg cacatgtccg gggcgaaaac cgggtacttg tgctttttta
     6901 ccgatttctt tcagggcctt ttcgaatggc tccttataat ctgcatggtc gagggtgata
     6961 tgcacaatgt ggtgctgact gcctaagttt tctacgttaa cgttcactgc tttggattga
     7021 aatggttatg ataagatctt ttcggaagct ttaaccacaa aggcggattt ccggttcccg
     7081 gaggtggtgc gggtggaggg actcgaaccc ccatgccttg cggcgccaga tcctaagtct
     7141 ggtatgtcta ccaatttcac cacacccgcc ttcgcttagc cctgaaaaag gaatgcaaag
     7201 gtaggaaaaa aaagggaatg ggaatgtgaa aatgtgggaa tgtgggaatg tgggaatgtg
     7261 agaatgtggg aatgtgagaa tgcttgcctc gctgcaggcg ggttcggcag tgtggaggac
     7321 gctgagggtg ctgtgaagga gtatcacaca ccactactca ccactcacca ctcaccattc
     7381 accctacaat ctcactcaat tcgagccatc gcaatccttt ttcatcgagt gcttcattga
     7441 tggcggcgag ctcggtggtc acctcgagga gcttgacgtg gtcggcggct acttcgataa
     7501 gccgggcttc gagttgcaac ttctgtatct cgagttccgg gagttctttg gataaaagtt
     7561 cgaactctcg cttttcgttg aaggtgggtt ttcgcttggc agtgacaacc acaggctcct
     7621 ggatcgacga tctttcagat tgttttgtga tggtcgatgt tgaagattcc gccaccggat
     7681 ccggaccttg cttttgcttg cgctgtaatt cgtcttgtgc atcgcgccac tcggtgtagt
     7741 ttccgggaaa atcgctgatg acgccattgc cttcgaaaac aaatacgtga tctaccaagc
     7801 gatccataaa ataacggtcg tgagaaacga tcagcaggca acccttgaaa tctgcaagga
     7861 attcttcgag aatgctcagc gtcaggatgt cgagatcatt cgtaggttca tctaagatga
     7921 ggaagtttgg atttttgatg aggatggtca tcaggtataa cctgcgtttt tcgccaccac
     7981 tcaatttact cacaaaagta tattgcgttt cgggcggaaa ctgaaacttg gtaagtaatt
     8041 gcgctgcact gagcttagat ccatcggcga gtggaatgaa ttcggcgatg tctttcacta
     8101 cttcgatcac gcgtttgtct tccttcaaca ccattcccga ctgcgagtaa taaccataca
     8161 ccacggtctc gccggtagtg atctttcctg catccggttt ttcgagttcc atgatcaggt
     8221 tgaggaatgt ggtttttccc attccattct tcccaatgat gccgatcttg tcgtattgct
     8281 tgaataaata ggtgaactgc tctacaattt tcagagtgcc aaatgctttt gacactttga
     8341 tcatctcaag gatcttgccg cccatgcgtg tcatttttac atcgagggtg agggcttcct
     8401 gcttttgttt tccttttgct ttttcggcga tctcatcgaa cgcatccagg cgcgatttgc
     8461 ttttggtggt gcgggctttg ggcatgcggc gcacccattc caactcgcga cgataggtat
     8521 tgcgcacctt ctctaattca gctgcttcca ccatatcgcg atgcgctttc ttttctacat
     8581 agagcgaata atttccacga taactaaaaa cttttccatt ctctaattca atgatctcat
     8641 tgcacacgcg atcgagaaaa tatctatcgt gcgttactaa caataaagtg atattcctcg
     8701 atgtcagata ctcttccagc cactccacca tttcaatatc aaggtggttg gtaggctcat
     8761 caagtatcat catatccggt tcttctatca gtaatttgct cagcgcgata cgtttttttt
     8821 gtccaccact caaagtgctc accttctgat caagctcggt gatattgagg tgactgagta
     8881 tctgtttgat acgcaattca taatcccagg cattaagcgc atgcatcgca tcgtgtgcgc
     8941 gttcgagagc ggttgatgtt tcttcgttga aatgatgttc ctgagccatc aaggcttctt
     9001 cgtatgcccg gattgcgcgg atggaagggg agtcgccact cagcacatta tcgatgatac
     9061 tgagcgactc atcgagcatc ggatcctgct ctaaaatacc cagggtgata ctcttatgca
     9121 cagaaacttt accggcgtcg gcaaagtctt ttcctgtcaa cactctcaac aacgtggtct
     9181 ttccggcgcc atttctcgcc accagcgcca ctttctgacc cctattgatc gtaaacgaaa
     9241 tattatcaaa taacaccttg gtgtcgtatg tcttggataa accctctgcc tgcaaataat
     9301 tcacgcggca aaggtaggga atgtgggggg atagggtggg gaattagcaa ttgaaggatt
     9361 ccaaaattct attgcaataa ttgaaagcta aattggagct cggcttcatt tcttcgaaga
     9421 aatcccgcca agcctataca ggagttgaga caacacctgt ttagcaccca tgtgtttagg
     9481 agtttgtcat gattttacct cagtcaatca ccctcacacg caacctacct tatgcaaagt
     9541 gcatcctcac ttatttcaga tcgctcaatc cgcccggtta gcatcttgct gtgttttcac
     9601 tccgttcact cggaatagct aacttaagtc taccggctgc tggcatttta ggctcgcctt
     9661 cgatgtagaa tgggctacga ggttgacatg gtttttgagt caaaaacaca cgccaacccc
     9721 ttgtgtagtc cgaaggctta tgccttcttc tacataggtc gcaaccg
//
LOCUS       DAOQOU010000126         9649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3773_length_9649_cov_4.921618, whole genome shotgun sequence.
ACCESSION   DAOQOU010000126 DAOQOU010000000
VERSION     DAOQOU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3773_length_9649_cov_4.921618"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1728)
                     /locus_tag="PLM90_10630"
     CDS             complement(193..1728)
                     /locus_tag="PLM90_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMZ89874.1"
                     /translation="MQEIIEVVISPEKVADDQAIFKTLEDVCGFAPAHWKIRKRSLDA
                     RGRSPVYRMQIIVSSAPIVELAYQRPAFKSIKDAAPVIIIGAGPAGLFAALTCIKQGI
                     KPVIFERGKDVRKRRHDLAALNRDGIVNPDSNYCFGEGGAGTYSDGKLYTRSDKRGNI
                     REVLETLVQFGADENILVDAHPHIGTNRLPKIIENIRNYILDAGGEIHFESKVSELMI
                     TDNVIKGIKLANGETHHAHAVILATGHSARDIFFMLHKQQIQIEAKPFALGVRIEHPQ
                     SLIDSMQYHCADRGPYLPPSPYSFVEQVNGRGVYSFCMCPGGIIAPCATAPGEVVTNG
                     WSPSKRNNPFANSGFVVGIELKDMREFEQYGALAGLYFQMAAEKAAFKAGGGKQKAPA
                     QRMVDFVDKKLSATLPDCSYHPGITSAPLEEVLPVFVANSLRQAFVQIRKKRPAYFTN
                     EAVCVATESRTSSPVRIPRDPQSLQHPQISGLYPCGEGAGYAGGIVSAAMDGIRIVER
                     MKG"
     gene            complement(1762..2706)
                     /locus_tag="PLM90_10635"
     CDS             complement(1762..2706)
                     /locus_tag="PLM90_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89875.1"
                     /translation="MRKLLALLTICIQTYCLSAQFLWEDECDFEETCPYLKPINDTSI
                     WEIGNPDKKTLNNCWNSPNCLVTDEILSVDTLNTDFIQFDIPCKTDGGEIPTGMTINF
                     FYRYDFPSPNDYCKVLMAFDSSEFKNVVYWSEQYENEYFSSIISDIPGYVYNETDTGI
                     TGLQTAWRDAYIFIIFYMTASPNGNELPDEDTVRLRIQLQTDSIDNKTAGIAIDEFSY
                     KFHIWGNVQDYHATTSLHISPNPVSANCQLFDASLKNTIQRLDIYNMTGQLMQTFPNV
                     VFDASGRAVLSLEALPSGTYICDIASDQSNYRGVVVKL"
     gene            complement(2742..3872)
                     /locus_tag="PLM90_10640"
     CDS             complement(2742..3872)
                     /locus_tag="PLM90_10640"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HMZ89876.1"
                     /translation="MEKVHERLQRELQTREEQHALRKLSVTDDLIDLASNDYLGFAKD
                     AQLRAMIDEEVKSLPFVGSGGSRLLTGNNAYVELVEKEICEFHQSDAALFFNSGYEAN
                     SGLISCVVHRHDIILYDELIHASLREGIKLSNAKAYAFLHNDIHDLKAKLEMHKGNVF
                     IITESVFSMDGDLCPLIEIIEIAEQFQAAIILDEAHATGVIGERGEGLAQYLGVQDSI
                     FARVHTFGKAIGANGAVILGSLILRDYLINFCKPFIYTTAPNYLQLAAVRMAYRFLRE
                     DTTPLQHLRANLELMKSLLEMSNFRKSVSTNSAIFSFVVPGNDRVKSLAAAMRSNGFD
                     MRPILSPTVPAGMERIRICVHAFNTHPEIEGAIRILASNMPN"
     gene            complement(3887..4861)
                     /gene="bioB"
                     /locus_tag="PLM90_10645"
     CDS             complement(3887..4861)
                     /gene="bioB"
                     /locus_tag="PLM90_10645"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HMZ89877.1"
                     /translation="MGDIRNNWTKEEISEIYYLPMMELIYRASTMHRKYHKTGEVQVC
                     TLLSIKTGGCAEDCAYCPQAARYHTDVEVHKLLEYDEVITKALIAKEFGSTRFCMGAA
                     WRNVRDNKDFDHVLQMVKGVADMGMEVCCTLGMLTEEQAVKLKDAGLYAYNHNLDTSE
                     EYYEAIITTRTYDDRLETIENVNKAEISVCSGGIIGLGESEDDRIGMLHTLSTLPKHP
                     ESVPVNALVPVKGTPLEHNKRVSVWEMVRMIATARIVMPKAMVRLSAGRNEMSVEEQA
                     LCFMAGANSIFAGEKLLTTPNPDADEDKAMFQLLGLSPRPAYKEEPVS"
     gene            4909..5331
                     /locus_tag="PLM90_10650"
     CDS             4909..5331
                     /locus_tag="PLM90_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89878.1"
                     /translation="MAFMHQFNYKHLIGLLLAGAVVLGTGELKDKKADKAPSEEVQAL
                     DAKLLKDLQHQEIQIDVKTATLMECGDVTESELRKLFDINNVNYNKCESGNCHYTSYT
                     IEGKLDNGTEVLFKLDSGEDGNVLRDLTFQGETIPCAM"
     gene            complement(5301..6182)
                     /locus_tag="PLM90_10655"
     CDS             complement(5301..6182)
                     /locus_tag="PLM90_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMZ89879.1"
                     /translation="MRSVIHWILYSNLLIAVAAAAYTLNGSMQMDLSQNIDPVLTWFV
                     GSSTLFIYLLIRYAAVSRIQEYTESDRWNFFLKYLHVFKVILIISGILSFVLYLFLPR
                     STELILLFPGGISMLYGLPIRFGKKKIRLRDIGIIKIFLISFVWAFVSSILPAVYVDV
                     NWRSPEVLWIFLANYIYIFAITLPFDIKDMEIDAMHHVRTIPVLIGKTMTISLSMLLL
                     FVSAMIYDLYYTVWFIRDISLEVPHIISILITGMTILLANNIKRDTIYFGLLDGMIIL
                     QFLLVGYSYMAQGIVSP"
     gene            6227..7393
                     /locus_tag="PLM90_10660"
     CDS             6227..7393
                     /locus_tag="PLM90_10660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HMZ89880.1"
                     /translation="MEVILQRGKARRVEDGHPWVYGNEIDVITGEPQTGDIVDVFNFK
                     KDFIGRGYINTKSQITVRILTRQRSEPIDISFFRKRLFACREYREKLGFTLNYRLVFG
                     EADFLPALVIDKFDDVYVMQTLSAGMDKWKRVIADILLDDFGASGVYERNDVPVRTLE
                     GLEETTGFLSNEFDTRFVIDEEGVKFYVDLVNGQKTGFFLDQKENRLALQHIVKNAEV
                     LDCFTYTGSFALYAAKFGARHVTALDISAEAIRLMQENVALNNFTNVEGVCANAFDVL
                     PVWVKENKQYDVVILDPPAFTKNRKGIENAVKGYKEINLRGIRMVRPGGFLATFSCSH
                     FMDVNLFYDTVASAAKDAGRTVREVQFLNQSKDHPVVWGIQETHYLKGLILQII"
     gene            7411..8256
                     /locus_tag="PLM90_10665"
     CDS             7411..8256
                     /locus_tag="PLM90_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HMZ89881.1"
                     /translation="MKNIYTFLALCILSSTAFAQTPSCCAAVGQFANFSHDIAFVKTH
                     EDPIPFQLTGQLGKMQTFACKDSSFATVYTVQAEQPSNKYLLVFHEWWGLNDYIKQTS
                     DKLYTDLGKNVHVIAVDLYDGKVAANKDSAASYMQTLTYDRAAMIINSLMDKFGDDVE
                     IATIGWCMGGGYSLQASLMAKERAVGCVMYYGFPETDKSKIANLSSDVLMIWPNQDKW
                     ITKEVVDKFTSDMQAAGKNLQVEEYTADHAFANPSNPKHDPVITADANAKALLFIKTH
                     LKIAD"
     gene            complement(8330..8860)
                     /locus_tag="PLM90_10670"
     CDS             complement(8330..8860)
                     /locus_tag="PLM90_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ89882.1"
                     /translation="MEQIIIATGIGAFGGWAYWRWIGCSSGTCAITKNKYMSILYGAL
                     LGLMVVTSGGCKSSNDTDVSKTGTTIQTASIFENISPAAFADKLQEPGVMLIDVRTPE
                     EFAEGHLAGAINMNINDSGFESSLARLDKSKTYLIYCRSGARSGRAGNLMASQGFTTI
                     YNLDGGILAWKGEVVK"
     gene            complement(8863..9168)
                     /gene="trxA"
                     /locus_tag="PLM90_10675"
     CDS             complement(8863..9168)
                     /gene="trxA"
                     /locus_tag="PLM90_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ89883.1"
                     /translation="MSTTSAFSELINGDQPVLVDFFAEWCGPCKTMAPILRDFAGEKS
                     GKVRVIKVDIDKNPGVAGQYQIQGVPTLILFKAGKPVWRQSGVVPLSYLHQAVDKVI"
     gene            complement(9223..>9649)
                     /locus_tag="PLM90_10680"
     CDS             complement(9223..>9649)
                     /locus_tag="PLM90_10680"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ89884.1"
                     /translation="LVEQGFTNITVIDIAEPPVNHLREALGADAPQQCNVVLGDFFAH
                     AGKYDHIVEQTFFCAIDPALRPAYVKHMAQLLKPKGELAGLLFKTPFDKPGPPYGGTE
                     AEYREVFAGSLNIIEMIPCEHSHPARAGNELFFRAGLLS"
BASE COUNT         2865 a         2106 c         1911 g         2767 t
ORIGIN      
        1 gctcacgagt cacccttgtt taatcgcgga gtttgcggag ttagcggagt cagagggtca
       61 gtcacgagtc acgactcacg attcacgatt cacccttgtt taatcgcgga gtttgcggag
      121 ttagcggagt ctgagggtca gtcacgattc acgacaatat atctatatcc gaagcaaaac
      181 caccattcaa tttcaaccct tcatccgctc tactattcga ataccatcca tagcagcgct
      241 cacgattccc cctgcataac cggctccctc tccgcatgga tataacccac taatttgcgg
      301 atgctgcagg gattgtggat cacggggaat gcgcacaggt gatgaagtgc gtgattctgt
      361 agccacacaa acagcttcat tggtaaaata ggccggtcgc tttttccgaa tttgaacaaa
      421 ggcttgtcgc aggctattgg caacaaatac cggcaacacc tcttcaagtg gcgcagaagt
      481 aattccggga tgataggaac aatcgggtaa agtagcagac aactttttat cgacaaaatc
      541 taccatgcgt tgagcgggtg ctttttgttt gccgcctcct gctttaaatg cagctttttc
      601 cgctgccatc tgaaaatata atcctgctaa agcaccatat tgttcaaatt cgcgcatgtc
      661 ctttaattct attcccacaa caaatccact attggcaaag ggattattgc gtttgctcgg
      721 actccagcca ttggtgacca cttcaccggg agcagttgca catggtgcga taataccacc
      781 ggggcacata caaaaagaat atacacctcg accgtttacc tgctcaacaa aactgtatgg
      841 cgaaggcggg agatatggtc cgcgatcagc acagtgatat tgcatgctat cgataagtga
      901 ttgcggatgt tcaatgcgca cacctaatgc aaatggtttt gcctcaatct gaatctgttg
      961 cttatgcaac ataaagaaaa tatcgcgcgc cgaatgtcct gtggcaagta tcacggcatg
     1021 tgcatgatgc gtttctccat ttgcaagttt tataccctta atgacattat cggtaatcat
     1081 caattctgac accttggatt caaaatgtat ttctcctccg gcatcaagaa tataatttct
     1141 gatattttca ataatctttg gaagtctgtt cgtgccaata tgcggatgtg cgtcgacaag
     1201 aatattttca tctgcaccaa attgcactaa tgtttctagt acttctctga tattcccgcg
     1261 cttatcgctt cgcgtgtata atttgccatc gctgtatgtg ccggcaccac cttcaccgaa
     1321 gcaataatta ctatcaggat ttacaatgcc atcgcgattg agcgcagcaa gatcatggcg
     1381 acgtttgcgc acatcttttc cgcgttcgaa aataacgggt ttgatgcctt gtttgataca
     1441 ggtcaaagct gcgaaaagtc ccgcaggtcc ggcgccgatg atgattaccg gagcagcatc
     1501 cttaatagat ttaaatgcag ggcgctgata tgctaattct acaattggcg cgctgcttac
     1561 gataatttgc atacggtaaa ccggtgatcg acctctggca tcgagagatc gcttacgaat
     1621 tttccaatgt gcaggagcaa acccgcagac atcttccaag gttttaaaaa tagcctgatc
     1681 gtctgctact ttttcggggg agataacaac ttcaataatt tcctgcatga aatataagtc
     1741 ccatcaattg ggactacaaa tctataactt cacaacgaca cctctgtaat tcgactggtc
     1801 agaagcgata tcacatatat atgttcccga tggcaaggct tccaaagaaa gcaccgctct
     1861 tcccgatgca tcaaatacaa cattggggaa tgtttgcatt aattgcccgg tcatattata
     1921 aatatcaagc ctttgtatgg tattcttgag agatgcatca aatagttggc aattagcaga
     1981 aacaggattt ggtgaaatat gtaatgatgt ggtagcatga taatcttgta catttcccca
     2041 aatatgaaat ttataagaaa attcatcaat tgctattccg gccgtcttgt tgtcaattga
     2101 gtcagtttgc aattgaatgc gtaatcgtac ggtatcctca tcaggcaact cattaccatt
     2161 gggcgacgca gtcatataaa atataatgaa aatatatgcg tctctccatg cagtttgaag
     2221 acctgttata cccgtatcag tttcgttata tacataaccg ggaatatctg atattataga
     2281 actaaaatat tcgttttcat actgttcact ccaatataca acatttttga attccgaact
     2341 atcgaaagcc atcaacacct tacaatagtc gtttggtgaa ggaaaatcat atcggtaaaa
     2401 aaaattaatg gtcatgccgg ttggtatttc accgccgtca gtcttgcagg gtatgtcaaa
     2461 ttgaatgaaa tctgtgttca aagtatctac agaaagtatt tcatccgtta ctaagcaatt
     2521 gggagaattc caacaattgt tcaatgtttt cttatccgga ttaccaattt cccaaataga
     2581 ggtatcgttg ataggtttta aataaggaca agtttcttcg aagtcacact cgtcttccca
     2641 aaggaattgg gccgacaaac aatacgtctg aatacaaata gttaatagag caagtaattt
     2701 tctcatggcg gggaatgctc ttcaaatata ctaaaagatc atcaatttgg catattcgat
     2761 gctaatattc tgatcgcacc ttcaatttcg ggatgcgtat taaaggcatg cacacaaatc
     2821 cttatccttt ccatacctgc aggaacggtg ggcgataaga tgggacgcat atcgaagcca
     2881 ttggagcgca tggctgctgc cagtgatttg acacggtcgt tgccgggaac cacgaaactg
     2941 aagatggcag aattggttga aacagacttc ctaaaattcg acatttccag caaacttttc
     3001 atcaattcca aatttgctct caaatgttgc aatggcgttg tatcttccct cagaaagcga
     3061 tatgccatgc gcactgcagc caattgcagg taattcggcg cagtggtata gatgaatggt
     3121 ttacaaaaat tgatgaggta atcacgcaaa atcagacttc ctaaaataac ggctccattt
     3181 gcaccaattg ccttaccaaa agtgtgtaca cgagcaaaga tgctatcctg aacaccaaga
     3241 tattgtgcca gcccttcccc tctttcacca atcacacctg tagcatgtgc ttcatccaat
     3301 atgatggctg cctgaaactg ctctgctatt tcaatgattt caatcagcgg acataaatca
     3361 ccatccatgc tgaacacaga ctctgtaatg atgaaaacat tccctttgtg catttccagt
     3421 ttcgccttga ggtcatgtat atcgttatgc aggaatgcat atgctttagc attgctaagc
     3481 ttaattcctt ctctgagcga tgcatgtatc aactcatcat aaagaatgat gtcatggcgg
     3541 tgtaccacac acgaaattaa tccgctgttt gcctcgtaac cactgttgaa aaatagtgct
     3601 gcatccgact gatgaaattc gcagatttct ttttcaacca actctacata ggcattattt
     3661 ccggtaagca atcgagatcc gccgctgcca acaaatggca atgatttaac ttcttcatca
     3721 atcattgctc ttaactgagc atccttcgca aatccgagat aatcgttaga tgccaggtct
     3781 attaaatcat ctgttacaga taatttgcgc agtgcatgtt gttcttcacg cgtttgaagt
     3841 tctcgctgta aacgttcatg cactttttcc atcattttcc aattaatcaa cttacaggct
     3901 cttccttata tgcaggtcga ggtgaaagac ccagcaattg aaacatcgcc ttatcttcat
     3961 cagcatcagg attaggcgta gtcagtaatt tctcaccggc gaaaatacta ttcgctcctg
     4021 ccataaaaca taatgcctgt tcttcaacgc tcatttcatt tcttcctgca ctcagtcgca
     4081 ccattgcttt aggcattaca atacgagctg ttgcaatcat gcgcaccatt tcccaaacgg
     4141 aaacgcgttt attatgctcc aatggtgttc ctttcactgg aaccaaggca tttaccggaa
     4201 ccgattcagg atgttttggc aatgtagaaa gtgtatgcaa catacctatg cgatcatcct
     4261 ccgattcacc aagaccgatg atgccgccgc tgcacacaga gatttctgct ttgtttacat
     4321 tttctatcgt ctccaaacga tcatcgtaag tacgagtagt aatgatcgcc tcataatatt
     4381 cttcactggt atccagattg tgattataag catataatcc ggcatctttt aatttcaccg
     4441 cttgctcctc tgtgagcatt cccaaagtac agcaaacttc cattcccata tccgctacac
     4501 ctttcaccat ttgcaatacg tggtcgaaat ctttattgtc gcgaacattg cgccaagccg
     4561 cccccatgca aaatctggtg gaaccaaatt cttttgcaat tagcgccttg gtaatcacct
     4621 cgtcatattc caacagttta tgtacctcca catcggtgtg atagcgtgct gcttgcggac
     4681 agtaagcgca atcctctgca catccacctg ttttgatact caacaaagta caaacctgca
     4741 cttcaccggt cttgtgatat ttacgatgca tggttgatgc acggtatatc aattccatca
     4801 tcggcaaata atatatttcc gaaatctcct cttttgtcca gttgttacgg atatctccca
     4861 tgagctgctt ttggagcaaa attcgtaatt ttgaggcata tttccgttat ggctttcatg
     4921 catcaattta attataagca cctgatagga ctgttactgg ctggtgctgt ggtattggga
     4981 accggcgaat tgaaagataa aaaggccgac aaagccccat cagaagaggt tcaggcgctc
     5041 gatgccaaat tgttaaaaga ccttcaacat caagaaatac agatagatgt taaaactgca
     5101 acattgatgg aatgtggcga tgtaacagaa agcgagttac gtaagctttt tgacatcaat
     5161 aacgtcaatt acaataaatg cgaatcagga aattgtcatt atacttcgta tacgattgaa
     5221 ggcaaacttg acaatgggac tgaagtgctg ttcaaactgg atagtggtga ggatggcaat
     5281 gtgctgcgcg atttaacatt tcagggagaa acaattccct gtgccatgta actatatcct
     5341 accagcaaaa actgaaggat gatcatacca tccaataatc cgaaataaat cgtatcgcgt
     5401 ttaatattgt tcgccaataa aatggtcatg ccggtaatta ggatactgat gatatgtggc
     5461 acttccaaag aaatatcacg gatgaaccac acagtgtagt aaagatcata tatcatcgct
     5521 gagacaaaca acaataacat cgataaactg atagtcattg tttttccaat caacaccgga
     5581 atggtgcgca catgatgcat tgcgtcaatt tccatgtcct ttatatcgaa aggaagtgtg
     5641 attgcaaaaa tgtatatata attagctaag aatatccaca acacctcagg gctgcgccaa
     5701 tttacatcaa catacacagc aggtaaaatt gaagatacaa aagcccaaac aaatgatatc
     5761 aaaaatattt ttataatacc aatatcacgc aaacgtattt tctttttgcc aaagcgaatg
     5821 ggcaatccat ataacataga tatgcctccc gggaacaaca aaatgagctc agtcgatctt
     5881 ggcaagaaca aatacaatac aaaacttaat atccccgata ttattaatat caccttaaac
     5941 acatggagat acttcaagaa gaaattccat ctgtcgctct cagtatattc ttgaattctg
     6001 ctaacagctg catatctgat cagcaaatag ataaacaagg ttgaactgcc gacaaaccat
     6061 gttaaaacag ggtcaatatt ttgcgacaaa tccatttgca tactgccgtt cagcgtataa
     6121 gccgccgcag caacagcgat cagcaaattc gaatacaata tccaatgaat gacggatctc
     6181 ataagcagcc gttgtaattt cgtaatttca cccaaattta cagaaagtgg aggttattct
     6241 tcagagagga aaagcacgtc gcgttgaaga cggtcaccct tgggtgtatg gcaacgagat
     6301 cgacgtgatc accggtgagc ctcaaaccgg cgatattgtt gatgtattca atttcaagaa
     6361 agattttatt ggtcgcggat atattaacac gaaatcgcaa attaccgtgc gcattttaac
     6421 acgtcaacgc agtgagccga tcgatatctc ctttttcaga aaacgcctct tcgcttgtcg
     6481 cgaatacaga gaaaaattag gattcacact gaattatcgt ttggtattcg gagaagctga
     6541 ttttctgccc gcattagtga tagataaatt cgatgatgta tatgtcatgc agaccttaag
     6601 tgcaggcatg gataagtgga agcgtgtaat cgccgatatt ttgcttgatg attttggagc
     6661 atcaggtgtt tatgagcgaa atgatgttcc tgtaaggaca cttgaaggat tggaagaaac
     6721 aacaggattt ctttcaaatg aatttgatac gcgatttgtc attgatgaag aaggcgtaaa
     6781 attttatgtt gatttagtga atggccaaaa gacaggtttc tttttagacc agaaagaaaa
     6841 ccgtcttgct ttgcaacaca tcgtaaaaaa tgccgaagtg ctcgattgtt ttacctatac
     6901 aggttcattt gcactctatg cagcaaaatt cggtgccagg catgtaacag cgcttgatat
     6961 ttctgcggaa gcgattaggt tgatgcagga gaatgttgca ctgaataatt tcacgaatgt
     7021 tgaaggcgtc tgtgccaatg catttgatgt attaccggta tgggtgaagg agaataaaca
     7081 atacgacgta gttatcctcg accctcccgc atttaccaaa aaccgaaaag gcatagaaaa
     7141 tgcggtaaag ggatacaagg aaatcaatct tcgcggcatt cgcatggtac gtcctggtgg
     7201 atttctggcc accttcagtt gctcgcattt catggatgtg aatttattct atgatacagt
     7261 tgcatcagcc gctaaagatg caggccgaac tgtgcgtgaa gtacaatttt tgaaccaaag
     7321 taaagatcac cctgttgtat ggggaataca ggaaacacac tatctcaaag gattaatttt
     7381 acaaattata taatatcaaa tttataaggc atgaaaaata tctacacctt tcttgcattg
     7441 tgcatactct cgtcaactgc attcgcacaa accccaagct gttgtgccgc cgttggtcaa
     7501 tttgcaaatt tttcacatga tattgctttt gtaaaaaccc atgaggatcc gattccattc
     7561 caactgaccg ggcagcttgg caagatgcaa acatttgctt gcaaagacag cagtttcgca
     7621 accgtttata ccgttcaagc tgaacagcca tcaaataaat acttactcgt atttcatgag
     7681 tggtggggat taaatgatta catcaaacaa acttcagaca aattatatac tgatctggga
     7741 aaaaatgtgc acgtaattgc cgtggacttg tacgacggca aagtggctgc aaataaagat
     7801 tcggcagcaa gttacatgca aacactcact tatgatcgcg ctgctatgat catcaattcg
     7861 ctgatggata aatttggtga tgacgttgaa attgccacta taggatggtg catgggtggc
     7921 ggatatagtt tgcaggcttc attaatggca aaggaacgcg ctgtaggttg tgtcatgtat
     7981 tatggatttc ctgaaacgga taaatcaaaa atcgccaatt tatcatctga tgtattgatg
     8041 atatggccga atcaggataa atggattact aaagaagtgg tcgataagtt tacatcagat
     8101 atgcaagcgg caggaaaaaa tttgcaagtg gaagaatata ccgccgatca tgcttttgcc
     8161 aatccaagta accccaaaca cgatccggtt attactgctg atgcaaatgc gaaagcacta
     8221 ttgtttatca aaacacattt aaaaattgcc gactaaatca tatacaaagt caaaggccgc
     8281 tccttatcgg atgcggcctt ttaactaccc tgttgtatat gtaattaagt tatttgacta
     8341 cttctccctt ccaggcaagt ataccgccat cgagattgta tatagtcgta aaaccttgtg
     8401 atgccattaa gtttcctgcc cttccgctac gtgcgccgct cctgcaataa ataaggtaag
     8461 ttttagattt atctaaacgc gctaatgatg attcaaatcc ggaatcattg atattcatat
     8521 ttattgctcc tgccagatga ccttcagcaa actcctcagg tgtgcgcaca tcaattagca
     8581 taactcccgg ctcttgtaat ttatcggcaa aggctgcagg agaaatattt tcaaaaatgg
     8641 atgccgtttg gatggttgtt cccgtcttag atacatccgt atcgtttgac gatttacatc
     8701 cacccgatgt aactaccatc aaacctaata aagcgccata aagtatgctc atatatttat
     8761 tttttgtaat cgcacaggtt cctgaactac atccgatcca ccgccagtaa gcccacccgc
     8821 caaatgctcc aattccagtt gctatgatga tctgttccat gattaaataa ctttatccac
     8881 cgcctgatgc aaatagctca atggtacaac ccccgactgt cgccataccg gtttacctgc
     8941 tttaaaaagt attaaagttg gcacgccttg aatttgatat tgaccggcta ctcccggatt
     9001 tttatctata tcgaccttaa tcacacgcac cttaccagat ttttctcctg cgaaatcgcg
     9061 caatatcggt gccatcgttt tacatggacc acaccattca gcaaaaaaat ctactaaaac
     9121 aggctgatca ccattaatca attcgctaaa tgccgaagtt gttgacatat taatacaatg
     9181 tgcaaaggaa ggagaaagtt caacgattca ccgtgacttt tgtcacgaca gcaagcctgc
     9241 tctaaaaaac agctcgtttc ctgctcgtgc tgggtgacta tgttcgcatg gaatcatttc
     9301 gataatgttg agtgatcctg caaatacctc ccgatattcg gcttctgtgc cgccatacgg
     9361 tggaccgggt ttatcaaatg gtgttttgaa taataatcct gcgagttcac ctttgggttt
     9421 taacagttgt gccatatgtt ttacataggc cggtcgcaat gcaggatcta tggcgcaaaa
     9481 gaaggtctgt tctacgatgt gatcgtattt tccggcgtgt gcgaagaaat cacctaatac
     9541 tacattgcac tgttgtggag catcagcgcc gagtgcttcg cgtaaatgat ttacgggagg
     9601 ttcggcaata tcgataacgg tgatattggt aaatccttgt tctacaaga
//
LOCUS       DAOQOU010000127         9567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3819_length_9567_cov_6.114907, whole genome shotgun sequence.
ACCESSION   DAOQOU010000127 DAOQOU010000000
VERSION     DAOQOU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3819_length_9567_cov_6.114907"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLM90_10685"
     CDS             complement(<1..412)
                     /locus_tag="PLM90_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMZ89885.1"
                     /translation="MSNIRIEKDTMGTVEVPAHVYWGAQTQRSIMNFPIAQDINKMPK
                     EIIAAFAYLKKAAAMTNHQLGVLDKSKADIIATVCDEILEGKLNDQFPLVVWQTGSGT
                     QSNMNVNEVVAYRAHVLLGGSLMDEKKAVHPNDDV"
     gene            complement(704..1063)
                     /locus_tag="PLM90_10690"
     CDS             complement(704..1063)
                     /locus_tag="PLM90_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ89886.1"
                     /translation="MFLDLPHKRMDVYIHARKLLLECRKVTDRFPHEEKFVLALQIRR
                     AALSVFLNIAEGSSRFSKAERRRFYEISRSSIVELDAAFDIAETYGYTQAGLLSEIGE
                     LIVRLFQMLSKLINNSQ"
     assembly_gap    1324..1423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1460..2026)
                     /gene="frr"
                     /locus_tag="PLM90_10695"
     CDS             complement(1460..2026)
                     /gene="frr"
                     /locus_tag="PLM90_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMZ89887.1"
                     /translation="MHEEELSMVIDMATEQMQKAIKHLELELSHVRAGRASIVILDGV
                     RIDYYGALTPLNQVANLSTPDARTIAIQPWDKKMLSEIEKAILAANLGLTPMNNGDII
                     RLNFPPLTEERRRDLVKQVKHMGETTKISIRNFRKDGNEEIKKLQKDGLAEDMAKDGE
                     DRIQKLTDKFIAEVDKHLGVKEAEIMTV"
     gene            complement(2051..2764)
                     /gene="pyrH"
                     /locus_tag="PLM90_10700"
     CDS             complement(2051..2764)
                     /gene="pyrH"
                     /locus_tag="PLM90_10700"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMZ89888.1"
                     /translation="MNPPYKRILLKLSGEALMGGQQYGIDPVRLQQYAMEVKKAADAG
                     VQIAIVIGGGNIFRGMSGVGSGIDRAQGDYMGMLATVINGMALQSALENVGCYTRLLS
                     AIKMEQICEPYIRRRAMRHLEKGRIVIFGAGTGNPYFTTDTAASLRAIEVEADVILKG
                     TRVDGVYTADPEKDPTATKYDSISFREAIAKNLNVMDMTAFTLCAENNLPIIVFDMNK
                     EGNLAKLISGESVGTLVKN"
     gene            complement(2877..3755)
                     /locus_tag="PLM90_10705"
     CDS             complement(2877..3755)
                     /locus_tag="PLM90_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ89889.1"
                     /translation="MSHYLDIFIDAYRGFAHYFWQSILHPSWNNFFYWLIGISIVVWA
                     LEVIFPWRKNQALIRQDFWLDAFYMFFNIFLFSLIGYYAISNVFVELFNDFLSLFGIK
                     NGIAIEINALPFWLKITFAFLLKDFIQWNVHRLLHSNKYLWQFHKVHHSVKQMGFAAH
                     LRYHWMENVIYKSIQYIPLAMLGFGISDYLIAGLITLVIGHLNHANINLRLGPLKYIL
                     NNPQMHIWHHAKHIPEQYPNGINFGISLSIWDYLFGTNYMPYSGRDIELGFAGDEHFP
                     HDFGHQAIYPAGKKQD"
     gene            complement(3833..4357)
                     /locus_tag="PLM90_10710"
     CDS             complement(3833..4357)
                     /locus_tag="PLM90_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ89890.1"
                     /translation="MTTRSIIIAAVLLLLIGFISVKRIRAQGHVDSTAYDVMLSTLLK
                     HNVPEININIAAADTNAIFLDAREHKEYAVSHIPEAIWIGYDDFSLSRVDSIDHSQPI
                     IIYCSVGYRSEVITGKLKKAGFTNVQNLYGGIFEWVNEGHQVVDDQGTETTRVHAYSK
                     KWGVWLNKGEKVYD"
     gene            complement(4386..5189)
                     /locus_tag="PLM90_10715"
     CDS             complement(4386..5189)
                     /locus_tag="PLM90_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ89891.1"
                     /translation="MEIIGYLGALLVGLTLGMFGAGGSILTLPVLVYLFGIDTSLATA
                     YSLFIVGATALAGAIPNMRRGTISYRTAIIFSIPSLTAVYLTRAYLMPAIPETLFSVN
                     DTVITKDIALLIFFALIMIFAAISMIRHRKIPDHSHDPDSAFNYLMILAEGSIVGVVT
                     GLVGAGGGFLIIPALVLFARLPMKMAVGTSLLIIAIKSLLGFVGDIQSGQPIDWPFML
                     IFTGVTIVGIFIGSALGRVFDAQKQKGAFGWFVFIMGVIILIEELILGL"
     gene            complement(5315..6901)
                     /locus_tag="PLM90_10720"
     CDS             complement(5315..6901)
                     /locus_tag="PLM90_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMZ89892.1"
                     /translation="MSELKSTLDFTEEKDFNTSEEHKALDAIRSFKGNYPRQLWYLFF
                     SEMWERFSFYGMRGMLTFFMINQLFMDEKVANLQYGATQAFVYAFTFIGGMFADKILG
                     FRKSLFWGGILMITGSLILAINPQEFFFLGISFNIIGTGFFKPNISTMVGLLYKDGDS
                     RRDAGFSLFYSGINIGALLGGYACIAIGKGTMLGSVIPENLHWNVAFGLVAVVMTISL
                     LTFVRTRHSLGPIGLSPVNLRAQKGGLELSRRWPEYLVYVGALVCIPLIIVMVSTPRY
                     VDLFMNIIGPASLLYLGYEMTKHNKQENLKLLAALCFILFSVIFWAIYEQAGGSLSIF
                     ADNNLNNDFLGGLIQLDANGVNNAANALFVIIMAPLVGLLWVSMSKRKIEPNTVVKFG
                     ISFLFLGGAYFIYYSLRFFADDNGISSLDMFTLAYFVVTLGELCLSPIGLSIMTKLSP
                     TRLQGVMMGMWFLASAYGQYAAGLFGASIVADSPDATNTDKLISYTSGYYDFAIYAII
                     AGVVLLAISPLVRKLMGNVK"
     gene            complement(6933..8642)
                     /locus_tag="PLM90_10725"
     CDS             complement(6933..8642)
                     /locus_tag="PLM90_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMZ89893.1"
                     /translation="MNKGSKAKHPKGLYVLFFTEMWERFGYYLMIGIFALYMKDAWES
                     GGMGFSAEKKSDVYGTYIGLVYLTPFIGGLLADRLLGYRRSIFIGGSLMAAGYFGLSI
                     HDPNAFYISLLLIILGNGFFKPNISTLVGNLYNDDRYRANKDEGYNIFYMGINIGAFV
                     CNFVAAYLRINLGWGYAFIAAGIGMIIGLIIFASGTKHVKEADVLKPAKPGDMGTGVI
                     LASVLIPMFAFGVLGYIIPDNIFGTDTNDAFLFGCIPVILFFIYILFKSDKEDRKPVA
                     ALLSVYGCIVIFWAVFHQNGDALTVWAERYSDRELNGGAKSVAETMKIAQTVENHPSA
                     FYDDEKFKSYLDSLDARRIAFSKAGNMDSVKAISETITIRTGSHTYFANLPAEDLPQP
                     DSNLKLASTELFQSINPFWVVVLTPIVVGFFGFLRRRGKEPSTPTKIALGLLITGLSA
                     FIMVAAVKSANIESEKVSSMWLFATYGVITLGELFLSPMGLSLVSKLSPPRLTALMMG
                     GFFLSTSVGNKLAGILSSNFERAEDKSTFFLTNVFLALAAAAMLFALLRWLNAIMKEK
                     GLK"
     gene            complement(8842..>9567)
                     /locus_tag="PLM90_10730"
     CDS             complement(8842..>9567)
                     /locus_tag="PLM90_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HMZ89894.1"
                     /translation="QAGASLTYFADKQTDRNILGWEMPASWFQSINPLGIIILAPLFS
                     MLWVALAKRKREPASPLKMAIGLFLLCLGYIVIAFGVKGVDPSIKISMWFLVMLYLLH
                     TMGELCLSPIGLSMVSKLAPLRFASLLMGTWFLANAAANKLAGTLSALIPPGEEQFQK
                     AQAAGIDLNSILNGTTAATADQAKVMTEQAIPTAWPSLMGMEITNLYQFFMVFIVMSG
                     VAAFILFFLSGKLKTMMHTEDNN"
BASE COUNT         2941 a         2123 c         1735 g         2668 t
ORIGIN      
        1 ttacatcatc atttggatga acagcctttt tttcatccat caggcttcct ccaagtaaca
       61 catgtgcgcg gtaagcaaca acttcgttca cattcatatt gctttgcgtg ccactaccag
      121 tttgccatac cactaacgga aattgatcgt ttaattttcc ttcaagaatc tcatcacaaa
      181 ctgttgcgat aatatccgcc ttcgatttat cgagaacgcc aagttgatgg ttagtcatgg
      241 cagccgcttt tttcaggtaa gcaaatgcgg caatgatctc cttaggcatt ttattaatat
      301 cctgtgcgat aggaaaattc atgattgagc gctgtgtctg cgcgccccaa tacacatgag
      361 ccggaacttc tacggtgccc atggtgtctt tttcgatacg tatattactc atagtttatt
      421 gagagattta ttgtttatca tttaaatatt cgatcgtgaa tcgtgactcg tgagtcgtga
      481 gtgtagctcg tgggttgtaa ctatgattcg tgactaaata gtggcttggg actgttaatt
      541 acgactttta agcgtggctt gttggatttt taacctaaat acacgctatg taatttcata
      601 tttatccttc aaccagtaaa tttttacgca aattcaaatt aataaaaaaa ttctcgctga
      661 atgttggagc caatgccaat catcacatta aaaaatttgg ttgctattga ctattgttta
      721 tcaatttgct tagcatttgg aacaatctga ctatcaattc tcctatttcg cttagcagcc
      781 ctgcttgagt gtagccatag gtctcggcaa tatcgaatgc tgcatctagc tcaacaattg
      841 agcttctgga tatctcataa aatctcctgc gttcagcctt cgaaaaccta gaggaccctt
      901 ctgcgatgtt gagaaataca gaaagggccg ctcttcgtat ctgcaatgca agaacaaact
      961 tctcttcgtg cggaaaccta tcagtaacct tgcgacactc caataacaac tttcgcgcat
     1021 ggatatatac atccatacgc ttatgcggta aatcaagaaa cataagaact aattattaag
     1081 ggttaaacaa aaaatatata ttgcaatgca atcatgcatt caaaaaaatc attaatgtca
     1141 atcacaaaac caaacatcac acaagcgagc aacataacac accacactaa caaataccac
     1201 cccgcacacg aaccacatcc ctccgactca cactcacgat tcacgactca cgattcacga
     1261 ctcacgattc acgactcacg attcacgact cacgattcac gccctcacac tgtcataatc
     1321 tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcacgac tcacgattca
     1441 cgactcacga ttcacgccct cacactgtca taatctccgc ttccttcacc ccaaggtgct
     1501 tatctacttc ggcaataaat ttatctgtca gtttttggat tcggtcttcg ccatcttttg
     1561 ccatgtcttc ggcgagtccg tctttttgca atttcttgat ttcctcattg ccatcttttc
     1621 tgaaattgcg aatagaaatc tttgtggttt ctcccatatg ttttacctgc ttcaccaggt
     1681 cgcggcgacg ctcctccgta agtggcggga aattaagtct gatgatatcg ccgttgttca
     1741 taggtgtaag tccgaggttg gccgcaagaa ttgctttttc aatttcactc agcatctttt
     1801 tatcccatgg ttgaatggca attgttcgcg catcgggagt cgacaagttg gcaacttgat
     1861 ttaatggcgt taaggcgcca taataatcga tgcgcacacc atccagaatt acgatacttg
     1921 cacgaccggc gcgcacatgc gacaattcca attcaaggtg cttaatggct ttctgcattt
     1981 gttcagtggc catatcaatc accatactta attcttcttc atgcatagct gtataatttt
     2041 ctttacctga ttaatttttt acaagcgttc caacagattc accgctgatg agttttgcca
     2101 gattcccttc cttgttcata tcgaatacga tgatcggaag attattttcg gcacataaag
     2161 taaatgctgt catatccatc acattcaaat ttttagcaat ggcctctcgg aaacttattg
     2221 aatcatattt tgtagctgtt ggatcttttt ccggatccgc cgtatacaca ccatcaaccc
     2281 ttgttccttt cagaatcaca tctgcttcta cttcgatagc gcgcaaactt gctgcggtgt
     2341 cggtagtaaa atatgggttt cctgttccgg ctccaaaaat tactatcctt cctttttcaa
     2401 gatgcctcat ggcacgtcga cggatatagg gttcacaaat ctgctccatt ttgatagcag
     2461 acagcaatcg cgtatagcaa cccacatttt caagtgcact ttgcaaagcc atcccgttga
     2521 tgacagttgc cagcatgccc atataatcgc cttgtgctct gtcgataccc gatcctaccc
     2581 cactcatacc cctgaaaatg ttgcctccgc caataacaat ggcgatctgc acaccggcat
     2641 ctgcggcttt tttcacttcc atggcatatt gttgaaggcg gacagggtcg ataccatact
     2701 gctgaccgcc catcagggct tctccggata atttaagcaa tatccgtttg taaggtgggt
     2761 tcatcaagca caaagataaa acttcatggg cagaggttcg aaaaaatagc cgatttaaaa
     2821 gcaatactca tccgggaaaa tgacctcaat ggcacactcg ctagaataat gcttcgtcaa
     2881 tcctgctttt tccctgcagg atagatagcc tgatggccaa aatcatgtgg aaaatgttca
     2941 tcgcccgcga atcctaattc gatgtccctg ccggaatatg gcatatagtt ggtgccgaat
     3001 aaataatccc atatactcag gctgatgccg aaattgattc cattaggata ttgctcagga
     3061 atatgctttg catgatgcca aatatgcatt tgaggattgt ttaaaatgta tttaagcgga
     3121 cccagcctca agtttatatt agcatggttc aaatgaccta tcacaagcgt gatcaatcct
     3181 gcaatcaaat aatctgaaat gccaaaaccc aacatagcca gcggaatata ttgtatgctt
     3241 ttgtaaatca cattttccat ccagtgataa cgcaaatgag cagcgaatcc catttgctta
     3301 acgctgtggt gcactttatg aaattgccat aaatatttat tgctgtgcag aagcctatgc
     3361 acattccatt gaataaaatc tttcaataaa aatgcgaagg tgatcttcaa ccaaaacgga
     3421 agtgcattta tttcaatggc gattccattc ttaataccaa atagggataa aaaatcattg
     3481 aataattcta caaacacatt ggatatggca taatacccta tcagcgaaaa cagaaaaata
     3541 ttaaaaaaca tataaaaggc atcgagccaa aaatcttgac gaatcaatgc ctgatttttt
     3601 cgccacggga atatgacctc taatgcccaa acgacgatac ttatgccaat aagccagtaa
     3661 aaaaaattat tccacgaagg atgtaatatc gattgccaaa aataatgtgc aaatcctcga
     3721 taagcgtcaa taaatatatc caaataatgt gacatgtccg actgatttct ttactaaaca
     3781 cgccaaagat aatggcaagg caagcaatgc cgggtgacaa tcatcacatc aatcagtcat
     3841 ataccttttc gcctttattc agccatacgc cccatttttt tgaataggcg tgtactctgg
     3901 ttgtttctgt tccttgatcg tcaacaacct gatgcccctc gttcacccat tcgaaaattc
     3961 ccccgtagag gttttgaacg tttgtgaagc ctgctttttt caattttccg gtgataacct
     4021 cactcctata tcctactgag caataaatga taatgggttg ggagtgatct atgctatcta
     4081 cccgactgag agaaaaatca tcatagccaa tccaaatggc ttcaggaatg tgacttacag
     4141 cgtattcttt atgttcgcga gcatctaaaa atattgcgtt cgtatcagcg gccgcgatat
     4201 tgatattgat ttcagggaca ttatgtttca gtagtgtcga tagcatgaca tcataagcag
     4261 tgctatcaac atgaccttgt gcacgtatac gtttgacgga aataaatcca atcagcagca
     4321 agagtactgc agcaatgatt attgaacgtg ttgtcatacg tcgttttcga aacacaataa
     4381 taacatcaga gacctaaaat taattcttct atcagaataa tcacacccat gataaacaca
     4441 aaccatccga aggccccttt ttgcttttga gcgtcaaata ccctgccaag tgcagatcca
     4501 atgaatattc caacgatggt aacaccggta aaaatgagca tgaatggcca atctataggc
     4561 tgtccgctct gaatatcacc tacaaatcct aagagcgact taattgcaat gataagcaat
     4621 gaagtaccta cagccatttt cattggaaga cgtgcaaaca acacaagtgc aggaatgata
     4681 agaaatccgc ctcccgctcc gaccagtccg gtaaccacac ctacaatgga accttcggcc
     4741 aaaatcatca gataattaaa tgctgaatca gggtcatgag aatgatccgg tattttgcga
     4801 tgtctgatca tactgatagc agcaaaaatc ataatcagtg cgaaaaatat cagtaaggct
     4861 atgtctttgg tgatgaccgt atcatttacc gaaaataaag tttccggaat cgccggcatc
     4921 aaataagctc ttgttagata aactgcggtg agacttggaa tggaaaagat tattgccgtg
     4981 cggtaggata ttgttcctcg tcgcatattt ggaatagcac cggctaatgc agtagcaccc
     5041 acaataaaca gtgaatatgc agtagcgaga gacgtatcaa taccaaataa ataaactaaa
     5101 acaggaagtg ttaaaatact tcctccggct ccaaacatac ccagtgttaa gcctacaagc
     5161 aatgctccga gatagcctat tatttccatt gcggagcaaa ggtaggcgaa ttcagaagtt
     5221 tataatgtaa ctgttagcac aaatgatggt ggaaaacaaa gggctcaatc cctgaacgga
     5281 actgagccct ttgctaaaag caatatatct tcaattattt aacatttccc atcagcttgc
     5341 gcaccaatgg tgaaatggct agaagcacca ctccggcaat gatcgcataa attgcaaaat
     5401 catagtatcc tgatgtgtac gatatcagtt tatctgtatt tgttgcgtcc ggagaatccg
     5461 ccacgatact cgctccgaat aaaccggcag catattgacc ataggcactt gccaaaaacc
     5521 acatacccat cataacacct tgcaatcgcg ttggtgaaag ttttgtcatg atagataaac
     5581 ctattggcga caaacataat tctccaagtg ttacaacaaa atatgcaagg gtgaacatat
     5641 ccaatgaaga aatgccattg tcatctgcaa agaagcgtaa agaataataa ataaaataag
     5701 cgcctccaag gaataaaaat gatatcccga atttcacaac ggtattgggc tcaatttttc
     5761 gttttgacat ggacacccat agcaatccga ctagcggagc catgataatc acaaacagtg
     5821 catttgctgc attatttaca ccatttgcat ccagctgaat aagaccacca agaaaatcat
     5881 tatttaaatt attatcggcg aaaatactca acgaaccacc tgcctgttca taaatagccc
     5941 agaagatcac actgaataaa ataaaacaca atgcagccaa taattttaaa ttctcctgct
     6001 tattatgttt tgtcatttca tatcccagat aaagcaagct ggcagggccg atgatattca
     6061 taaacaggtc tacatagcga ggagtactca ccattacaat gatcagtgga atacaaacca
     6121 atgcaccaac atataccaaa tattcaggcc agcgacgaga taattccaag ccaccttttt
     6181 gcgcacgaag attcaccggt gaaaggccga taggaccaag tgaatgtctt gttcttacaa
     6241 atgtaagcaa gctgatagtc attacaaccg caacaagtcc aaaagctaca ttccaatgca
     6301 gattttccgg aattacactg ccaagcatag ttccctttcc gatggcaata caggcatagc
     6361 caccaagcaa tgcaccgata ttaattcctg agtaaaataa ggagaagcct gcatctcttc
     6421 tactatcacc gtccttgtat aataaaccta ccatggtgga tatattaggt ttaaagaatc
     6481 cggtgccgat tatattaaaa ctgataccca gaaagaagaa ctcttgcgga tttatggcaa
     6541 gaatcaaact ccctgtaatc atcaaaatac cgccccagaa taatgacttt ctgaatccaa
     6601 gaattttatc tgcgaacata ccgccgataa atgtaaaagc gtaaacaaaa gcctgcgtag
     6661 cgccatattg taagttggct accttttcat ccatgaacag ttgattaatc atgaagaatg
     6721 taagcatgcc gcgcatacca tagaaactaa agcgctccca catttcactg aagaacagat
     6781 accaaagttg gcgagggtaa ttgcctttga acgatcggat tgcgtctaag gccttgtgtt
     6841 cttctgaggt attaaaatct ttttcctcag taaagtctaa tgtagattta agttctgaca
     6901 tatatctgtt ttaatgcaaa ttgtaattgt ggttacttca aacccttttc tttcataatg
     6961 gcatttaacc atcgcaataa tgcgaataac atagctgctg ccgctaatgc taaaaatacg
     7021 tttgtcagga agaaggtgga tttatcttct gctcgttcga agttgctcga caatattccg
     7081 gcgagtttat ttcctactga tgtcgacaag aaaaatcccc ccatcatcaa tgccgttaat
     7141 ctaggtggtg ataattttga aacaagcgaa agtcccattg gtgataaaaa taattctccc
     7201 aaggtgatta ccccataagt agcgaataac cacatactgc ttaccttttc tgattctatg
     7261 tttgccgatt ttaccgcggc taccatgata aatgcactta agcctgtgat caggagtccg
     7321 agggcgatct tggtaggcgt tgaaggttct ttccctctgc gtcgaaggaa tccaaaaaat
     7381 ccaacgacaa tcggcgtaag cacaactacc cagaatggat tgattgactg aaacaattcc
     7441 gtgctggcaa gtttcaaatt gctatcaggt tgaggaagat cttcagcggg aagatttgca
     7501 aaataggtat gtgatccggt tctgatagtt atagtttccg aaattgcttt taccgaatcc
     7561 atatttcctg ctttgctgaa tgcaatacgc cttgcgtcaa gactatcgag atatgatttg
     7621 aatttctcgt catcataaaa tgcagaagga tgattttcga ccgtttgtgc aatcttcatg
     7681 gtttctgcta ctgacttcgc cccgccattt aattcacgat ccgaatatcg ttcagcccaa
     7741 acggtgagcg catcgccatt ttgatgaaat acagcccaga aaattacaat acatccataa
     7801 acacttaaca gcgctgcaac gggtttgcga tcttctttat ctgatttaaa caggatataa
     7861 ataaaaaata aaatcaccgg tatacaacca aataaaaacg catcattggt atcggttccg
     7921 aaaatattgt caggaataat gtaacccagc actccgaatg caaacatcgg tataagaacg
     7981 gaggcaagaa tgacgcctgt tcccatatct cccggtttag ccggtttcaa aacatcggct
     8041 tcttttacat gtttagtgcc ggaagcgaat atgatcaaac cgatgatcat cccgattcct
     8101 gcagcaataa atgcatatcc ccaaccgaga tttatgcgca aataggctgc aacaaaattg
     8161 caaacgaagg caccgatatt gatacccatg taaaaaatat tatagccttc atctttgttg
     8221 gcgcgataac gatcatcatt atataaatta cctacaagcg ttgaaatatt aggtttgaaa
     8281 aagccattgc ccaaaatgat caacaataaa gaaatataga aagcattcgg atcgtgaatc
     8341 gataatccga aatatcccgc tgccatcaat gaacctccga taaaaattga tcgtcggtag
     8401 ccgagtaatc tgtcggcaag taagcccccg ataaacggtg ttaaatacac cagaccgata
     8461 taggttccat agacatcaga tttcttttct gcgctgaatc ccattcctcc actttcccat
     8521 gcatctttca tgtaaagggc gaagatgcca atcatcagat aatagccaaa tcgctcccac
     8581 atttctgtga aaaagagcac atataggcct ttcgggtgct ttgctttgct gcctttgttc
     8641 attaattgcc tgtttttggg tgtctaaaga taaacatgat ttccatagag ccaaacgcct
     8701 ctcaagttcc ggaaagccaa aactgtgccg atttagcaaa gcttaataat ttgaagtagc
     8761 agatatcagg attatacatg aaataaaaaa gcagctaccg aatcaggcaa ctgctttttt
     8821 ttacaagaat atgaaaaagg attagttatt atcctctgta tgcatcattg ttttcaattt
     8881 gccgctgagg aagaaaagta taaatgctgc cacgccactc attacaatga agaccatgaa
     8941 aaactggtat aaattggtga tctccattcc cataagactt ggccatgcag taggaatagc
     9001 ttgttctgtc attactttcg cctgatcggc agtagcggca gttgttccgt ttaatatact
     9061 gttcaggtcg ataccggcag cttgtgcttt ttggaattgt tcttctcccg gaggaataag
     9121 cgcactcaat gttccggcaa gtttatttgc tgctgcgttt gcaagaaacc atgttcccat
     9181 caataaagat gcaaagcgca atggagccag tttactcacc atcgataaac caataggcga
     9241 taaacataat tcacccatag tatgcaataa ataaagcatt accaagaacc acatactgat
     9301 tttaatggaa ggatcaacac ctttcacacc aaatgcaatc acaatatatc caagacataa
     9361 taagaataaa ccaattgcca tttttaatgg tgatgcaggc tcgcgtttgc gtttagcgag
     9421 tgctacccac aacatgctga aaagaggggc aagaatgata ataccaagtg ggtttattga
     9481 ctgaaaccag cttgcaggca tttcccaacc taaaatattt ctgtcggttt gtttatcggc
     9541 gaaataggta agcgatgccc cggcttg
//
LOCUS       DAOQOU010000128         9546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3826_length_9546_cov_6.054367, whole genome shotgun sequence.
ACCESSION   DAOQOU010000128 DAOQOU010000000
VERSION     DAOQOU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_9546_cov_6.054367"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..937
                     /locus_tag="PLM90_10735"
     CDS             428..937
                     /locus_tag="PLM90_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89895.1"
                     /translation="MQNWIQTTILVIVILAISVALRKSAHAEAGKKDGMSLYKAPVIF
                     KYIGFGLLAFGVYVLGDTIFGNSINQHSMNYLLAGIFVATGGLLIYFQRTFYITIDAH
                     QVVKHKMFHKEITIQLNEIQYIRYRKLMKDMIIISANGKMIIDESVTGFMSLVNELKE
                     KTGVDIDNL"
     gene            1066..1578
                     /locus_tag="PLM90_10740"
     CDS             1066..1578
                     /locus_tag="PLM90_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89896.1"
                     /translation="MQLNIPGFIFMALLVFLVKKTSTDIPTVTDESQVLYRPSRSFGV
                     LGMLLFFLGIVLFTLILLGELPSNGYTGAFFLTFFCISGLLLTYFRMAVYLKTTGEAL
                     IKRNIFKKEIIIPYKEIVSLSYNKNISALIVKTEVEYLFCFNNLIGFNFFIQTLEEAT
                     GLEAKGWMTR"
     gene            complement(1634..2443)
                     /locus_tag="PLM90_10745"
     CDS             complement(1634..2443)
                     /locus_tag="PLM90_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89897.1"
                     /translation="MKIIKTVFLSALTISASQQLHAQDVHLHVNTQWEECSIQLDSTL
                     SQGAWHQFTKEAGMVVYFRPLHGAETLAPGKFELSVLQWNTGIDETQSAWNETFVHPD
                     TTHYLIGGDELPFPGLTLRAGITDKLDAGICWSKSPGANYSIAGAQVQYQVLGDTVKH
                     WNIATRLGFTSLYGPEDLGYVEFGLDMLASKEFPFMQDHLALTPYALVSTDFVHAHEK
                     SEIVTLDDENVMGAQAALGLEAKIYVVRLGVEYNLSAVNTLSYKLGVRVAF"
     gene            complement(2577..3725)
                     /locus_tag="PLM90_10750"
     CDS             complement(2577..3725)
                     /locus_tag="PLM90_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ89898.1"
                     /translation="MSLLLLTKKNKGAADRILLLWMVFITLHQLYYYFDHSAYFRTYP
                     AFVGFDFPLPLLHGPLLFLYAAALVGRLPEKWWQRFVHFIPALACLMYMTKFFMLTGS
                     EKYYIIDNEGVGYELFLLIRFIAIILSGIVYVVLTLILLNRHQRSIRENFSDIEHINL
                     KWLQYLTAGISLIWISVIFGTDEITFSIVVIFVLFIGIYGIRQTPIFASGHPLGTPIS
                     IVGEQVNNAIDHADTNIDAVDVIANEPQDIIAQTKYRKSGLSEGDIDALHRKLTDLML
                     KEALYTQPDITLGEVARRLDTHPNYLSQVINARLQKTFYDYINAHRVEAFTQLVSDPA
                     QRRYTLLALAFQCGFNSKSSFNRNFKKVMGESPSDYLDRMQVVLDGEV"
     gene            complement(4365..5813)
                     /locus_tag="PLM90_10755"
     CDS             complement(4365..5813)
                     /locus_tag="PLM90_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89899.1"
                     /translation="MAQPKLITIYGALSERERERFGEFVRSPFHNKRSKLINLVAHLE
                     VMFAGEEPKIWDKHEAYQCVFEADTPGKKRPAFDELQLNNVVSDLFRLMEDFLAWQQF
                     SRQPEWRDVMMVEHLLSSSMLEQADKVIRKKASNEMSDAYAQYRFNQLADQHFFQRSR
                     KGDNRYLIQGQQSLEIYFLANQLRIWCELINRANVLSLAYDKQQFADFEQYLLLSLPK
                     YGNQPTISIYWPILTWLRDQENDTWFIGFKEKLFQHIQEFPEQEGKDIIAYVQNYCVK
                     RINEGKNAFLREWYEIAQYMLPLRLLHEGQWISQWTYKNIVTAGVRLKEFAWTEQFAH
                     DYYTALSLEERDNAYQYNLAVIYYEKHDIDRAMQLLNRVHFSDPNYYLDAKTILLKIY
                     YEHNAFEALISLRDTVKIYLLREKLLSRSQQMLYKNLFSLTLKLYKLRLEKESTDPEK
                     WRKQFELLKKGISENNLVANKGWLMGKMEEVN"
     gene            5983..8184
                     /locus_tag="PLM90_10760"
     CDS             5983..8184
                     /locus_tag="PLM90_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89900.1"
                     /translation="MKRLLFLLFAISALSLTAQVPWSYAHQGTDTWPAANAKDLLVGA
                     DDETFVFGDIGLIDINEGAFTLAAIASDGSVVWEQTYDKPGTLEMGMRMSRTSDDGFL
                     LSGMTAPDYRPYFVRTDADGNEMWNSASVTDLLPENSASQCYAYELPSGNIAYVFSDD
                     VYKFLTRIEVDPATGAAISTTTLNYLDILPAGIGTVITANTVDMITNNSGDLIVIADF
                     MATGADTTDYIFGINSDFELAGRFGISNGAPNYYANSVIQNADGDYIITGTKNTGAFF
                     DLYSPMITLVQGGFLWSSTLSFTPTLSQATGGAITQTTEGGYKWIRYQQNGTYFDPAV
                     GDYVEVATLDMWLTETATHTINYAPYNTFECIAALAGTPAIDDAYVIGGSAWTIGVPA
                     YNLVIGTGAADAMPACIFNCVWPGDADNSGLVDMDDLLSIGLGFGNTGAARDAIDISW
                     TAHQAEAWATALPGGINHKYTDCNGDGIIDATDEDAVDINYGFDHEVNTMRMSEGDVP
                     LYYAPVDPIVIGENAIPIMLGDVATPVDAIYGLRFTATVDGESIDASSVYVTFNDSWI
                     GNTSEMLQLSKTLATPAADAGLVKTTNENANGYGEIGTLHFVVIDNIAGKITSDEITI
                     GFADARAINVNNEDLSLNTSELTVDVTLATNNPSQPAISIYPNPVSNNLIYTNLELVN
                     ARVTISDMVGREIVLEQVNGNTIQLPQLATGNYLMRITQSGGTSVFTLSLQ"
     gene            8415..>9546
                     /locus_tag="PLM90_10765"
     CDS             8415..>9546
                     /locus_tag="PLM90_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89901.1"
                     /translation="MKLRFTLIAFSALLLIGNRVSAQAWGYSNIGHWVSDMSVGSDGS
                     VWTVGYGGDDPAWYDVAADYVGKVNPDGTSALGFHMAIPGATTVYNFSIGATTEGGAA
                     VLSSTDILGWAVTKLDESGAIEWVNSGFESYYIYGGILAVLNDGKVVVGGLADFEHRL
                     YEFSSTGDLIGSYTIAPDTGAVWMVGYYNFKEAGITATADGGFAYATGGENNRLLYKF
                     DSDMNLEWTGDYPWEMEWEYPMQNGLKTTSDGGFLFSGSSTSAATFDYNGTVRKIAAD
                     GTLEWFNSYFHGSPYEEGTWAVELGSGDISVFTQDAGEASTNAWMTTATGTEYGAEFI
                     PVMNVIGGYSETGVDVWDIENAADGGLYIAGRMWLENYDQRQF"
BASE COUNT         2769 a         1878 c         2004 g         2895 t
ORIGIN      
        1 gtaaaactac tattattcaa cacctcttaa aatgaccgag atcattatga aattggtgta
       61 aatagatatt gcaggttagt gaattgattc ttacaaaatt ggatgattcc ttggcactac
      121 ttgaaaaatt ttaaggattt ggatgaatat tttgaaaaaa aaatattttt tgcggtttcc
      181 aatactttat aacatagggg tttggttttc cggaaatcca tcattggata tgacaattga
      241 gcggtatttg aaattatttg ccggaattgc gacatgcatt tattaatcga atttagtaac
      301 aaacttgcct aatacattta gattaaagag acgctaatta tgaaattccc gatatgtgtg
      361 gcaattttgg tatcattaca aatattcgct aggtacgcaa cattgcttct gcacataata
      421 aaagaggatg caaaattgga tacaaaccac aatccttgtt atagtcatat tggcaatttc
      481 agtcgcattg cgcaaatctg cacatgctga ggccggtaag aaggacggga tgtcgcttta
      541 caaggcacct gtcattttca aatatattgg ctttggatta ttagcatttg gggtgtatgt
      601 gcttggtgat acgatatttg gaaattctat aaatcaacac agtatgaatt acttgcttgc
      661 aggaatattt gtagcaacag gaggtttatt aatttatttt caaagaacat tttatatcac
      721 aattgatgca catcaagttg taaagcataa aatgttccat aaagaaatta cgattcaatt
      781 aaacgagatt caatatatcc gttaccgaaa actcatgaag gatatgatca ttatatccgc
      841 taacggaaaa atgataatcg atgaaagtgt taccggtttt atgagcctgg tgaatgagct
      901 aaaagaaaaa accggagtag atatcgacaa tttgtaggtt caggtgtagt atcttaacct
      961 gaagccaatt cgatgtattt tgtttcggat gatgtaactt tcggttgata atttggtgaa
     1021 tggtttcggc gagtaaattt cactagtgtc taaaacacat acaagatgca acttaatatc
     1081 ccgggattca tttttatggc cttgttggtg tttctggtta aaaaaacatc caccgacatt
     1141 ccaacagtta ctgatgaatc acaagtgctt tacagacctt ctcggtcttt tggtgtgtta
     1201 ggtatgttgt tgttctttct gggaattgtg ctgttcacgc ttatcctgct tggcgagttg
     1261 ccaagtaatg gatatactgg agcgtttttt cttacctttt tttgcatatc cggattgtta
     1321 ttaacttatt tccgaatggc agtttatctg aaaacaaccg gtgaggcatt aataaaaaga
     1381 aacatcttta aaaaggagat tatcatacca tataaggaaa ttgtttcact atcctacaac
     1441 aaaaatattt cagcccttat tgtaaaaaca gaggtcgagt atttattttg cttcaataat
     1501 ttgattggat ttaatttttt cattcagaca ttggaggagg caaccggatt agaggcaaaa
     1561 ggttggatga ctagatagca taaaaaagct gcgaggataa caccgtaaac cctatcctcg
     1621 caacttagat aaattagaat gcaacacgca cacctaattt gtacgacaat gtattaaccg
     1681 ccgacaaatt atattccact cccaaacgca ccacatatat ttttgcttcc agtcccagcg
     1741 ctgcttgagc acccattaca ttttcatcgt caagcgttac tatttctgat ttttcgtgtg
     1801 cgtgaacaaa atcggtgctg acaagcgcat aaggtgttag cgcgagatga tcttgcataa
     1861 atgggaattc tttacttgca agcatgtcca aaccaaattc aacataacca agatcttcag
     1921 ggccatacag cgaagtaaat cccaatcgcg ttgcaatatt ccaatgctta acagtatcgc
     1981 ctaaaacctg atattgcact tgggctccgg caatgctgta attggcaccc ggacttttcg
     2041 accagcaaat tccggcatca agtttatctg ttattcctgc gcgaagagta agtcccggaa
     2101 atggcaattc atcaccaccg atcaggtaat gtgtagtatc cggatgaacg aaggtctcgt
     2161 tccaggcaga ttgggtttcg tcgatacctg tgttccattg taaaaccgag agttcgaatt
     2221 tacccggagc tagggtttct gctccgtgaa gcggcctgaa gtaaacaacc atacccgcct
     2281 ccttagtgaa ctgatgccac gcaccttgtg aaagcgtgga atccagttgt atggaacatt
     2341 cttcccattg tgtgttaaca tgcaaatgca cgtcctgagc atgcaattgt tgtgaggcgc
     2401 ttatagttaa tgcagataaa aaaactgttt tgatgatttt cattttatac gatttatgcg
     2461 gcaaaggaac ggtgatcata agctcctgtc gcttcatctt ataagatgac accaataaat
     2521 acaggctttt gagcccgaat gcgtaaattt ttagaaatat taacgatagc cggggatcag
     2581 acttctccat ccaaaaccac ctgcatgcgg tcgaggtaat cggatggcga ttcacccatc
     2641 acttttttaa agttgcggtt gaacgatgat ttcgaattga aaccacattg aaatgcaagg
     2701 gccaatagcg tataccggcg ttgagcagga tcactcacca attgtgtgaa agcctccacc
     2761 cgatgtgcat tgatataatc gtaaaaagtt ttttgcaagc gcgcattaat cacctgcgag
     2821 aggtaattcg gatgagtatc aagtcttcgg gctacctcgc caagggtgat atcaggttgt
     2881 gtatataacg cctctttcag catcaaatcg gtcaacttcc gatgtagtgc atcgatgtcc
     2941 ccctcgctca atccggattt tcgatatttt gtttgggcga tgatatcctg gggttcattt
     3001 gcaataacat cgaccgcatc aatattggta tcggcatggt caattgcgtt gtttacttgt
     3061 tctccgacga tggaaattgg agtgccaagg gggtgtccgc ttgcaaaaat gggtgtctgc
     3121 ctaatgccat aaataccaat gaataaaaca aaaatgacca ctatcgaaaa cgttatttca
     3181 tcagtcccaa aaattaccga tatccatatc aagctgatac cggcagtgag atattgaagc
     3241 cattttaaat taatatgttc gatatccgaa aaattctcgc ggatgctgcg ctgatgtcga
     3301 tttaataata tcaaagtgag cacaacataa actattcctg ataaaatgat ggcaataaat
     3361 ctgattagca gaaataattc gtatcctact ccttcattgt cgatgatata atatttttca
     3421 gaaccggtca acataaagaa ttttgtcatg tacatcaaac atgccagtgc gggaataaaa
     3481 tgcacaaaac gttgccacca tttttccggc aatcgcccaa cgagtgcggc agcataaaga
     3541 aataacagcg gaccatgtaa caagggcaaa ggaaaatcaa agccaacaaa cgcgggataa
     3601 gttctaaaat aagcagaatg atcaaaataa taatacaatt gatgcagggt gatgaagacc
     3661 atccacagca gcaatatcct gtcggccgca cccttgtttt tcttagtgag caacagcagt
     3721 gacaaaaaca atgagattac aatgcctgca attattatca tcccccgcaa atctagctaa
     3781 acattgcagg caaatgcaaa ctcaatggtg agaggtgaga ggtgagtggt gagtggtgaa
     3841 tggtggctcg tgaattttta cctgatactt gatacttgat actaaattcc gaatactatc
     3901 acaatcctca aaaacccatt taaccacaga gaccacggag atgaacacag aggtgcacag
     3961 agtaaattcc cggcacattc caccgcattt atctcactac tcactaccca ctaccgaatt
     4021 gtgagcggta agtggtgagt ttgggcgcac tttagcatcg gatatatgat gcaaatatgc
     4081 atttacctta cacaccaatc accattcacg actcacgatt cacccttgtt caatcgcgga
     4141 gtttgcggag ttagcggagt cagtggatca gtcaccactc accattcacg actcacgatt
     4201 cacccttgtt caatcgcgga gtttgtggag ttagcggagt cagtggatca gtcaccacta
     4261 accattcact attcacgact ctcgagtcac gagtcacgat tcacgaaaca ctcacccact
     4321 caccattgca tttccatttg ctaatcagct aatttgctaa ttggctaatt aacttcctcc
     4381 attttcccca tcaaccatcc cttattcgca actaaattat tttcactgat tccctttttc
     4441 agcagttcaa attgcttgcg ccacttttca ggatcggtag attctttttc taaccgcagt
     4501 ttatataatt tgagtgttaa ggaaaagagg tttttatata gcatttgctg actgcgactg
     4561 agcaattttt ctcgtaataa atatattttc actgtgtcgc gaagagatat cagcgcttca
     4621 aatgcattgt gttcgtagta aatttttaaa agtatggttt tggcatccag ataataattc
     4681 ggatcgctga aatgtacacg gttgagtaat tgcatggcgc ggtcgatatc gtgcttttcg
     4741 tagtagataa cggcaagatt atattgataa gcattatcac gctcctccag acttaatgca
     4801 gtataataat cgtgagcgaa ttgttctgtc catgcgaatt cctttagtcg cactccggct
     4861 gtaacgatgt ttttataagt ccattgggag atccattgtc cttcatgcag caacctgagc
     4921 ggtaacatgt attgtgctat ctcataccat tcgcgaagaa atgcattttt accttcattg
     4981 atacgtttta cgcagtagtt ttgaacatag gcaatgatgt cttttccttc ctgttcaggg
     5041 aattcttgaa tatgctgaaa tagtttttcc ttaaatccta taaaccaggt gtcattttcc
     5101 tgatcgcgca gccatgtgag tatgggccaa taaatgctga tagttggttg attgccgtat
     5161 ttcggtaaac ttaaaagtaa gtattgttcg aaatcagcaa actgttgttt gtcgtaggca
     5221 agtgataata cattagcgcg attaattaat tcacaccaaa tgcgtaattg atttgccaga
     5281 aaatatattt caagtgattg ctggccttgt atcaaatagc ggttatcgcc ttttcgcgac
     5341 cgttgaaaaa aatgttgatc ggccaattga ttgaaacgat attgtgcata ggcgtcactc
     5401 atttcattgg aggctttttt tctgatcacc ttatcggctt gctcaagcat cgaagacgat
     5461 aataaatgtt ccaccatcat cacatcgcgc cattcgggtt gccgactgaa ttgctgccat
     5521 gccagaaaat cttccatcaa cctaaaaagg tcgctcacta cattatttag ttgcagctca
     5581 tcgaaagcag gtcttttttt gccgggggtg tctgcctcga aaacacattg gtaagcttca
     5641 tgtttatccc aaattttagg ctcctcgccg gcaaacatca cctccagatg agcaacaagg
     5701 tttatcagct tactgcgctt gttatgaaaa ggactgcgca caaattcgcc aaaacgctcc
     5761 cgttcccgct cggaaagtgc accgtaaatg gtaataagtt tcggttgcgc catggctata
     5821 aaattggcgt tatattaaac ataaagactt gaaaaacaat gttttaccta tatttgatat
     5881 gagcattttg gctataaaac atggttaaaa gtaattgttt ttacccggaa ctgtatcgtt
     5941 ctttgttgca atgatttcca taaacattaa atttataaca tcatgaagcg ccttcttttt
     6001 ctactattcg caatttcagc actctcgctt actgcccagg tgccttggag ttatgcccat
     6061 caaggtactg atacctggcc tgctgccaat gccaaagatc ttttggttgg tgcagatgac
     6121 gagacctttg tgtttggtga tatcggcttg atcgatatta atgaaggcgc tttcaccctc
     6181 gcagctatcg catcagacgg cagcgtggtg tgggaacaaa cttatgataa accgggtacg
     6241 cttgaaatgg gtatgcgcat gtcgcgtaca tccgatgatg gatttctgtt gagtggaatg
     6301 acagcaccgg attatcgccc atattttgtg cgcacagatg ccgatggaaa tgaaatgtgg
     6361 aattcggcat cggtaacaga tttacttcct gaaaattcag catcgcaatg ttatgcatat
     6421 gaattgcctt caggaaatat tgcttacgta ttttccgatg atgtatataa atttcttacg
     6481 cgtattgaag tcgaccctgc aacaggtgcc gcaatcagta ccactacatt gaattatctc
     6541 gacatacttc ctgcaggaat tggtactgtc atcactgcca atacagtaga tatgatcacc
     6601 aataacagtg gcgatctgat cgtgattgca gattttatgg caacaggtgc cgatacaacc
     6661 gattatattt ttggtattaa ttccgatttc gaacttgcag gacgatttgg tatttcaaat
     6721 ggtgctccca attattatgc aaatagtgta attcaaaatg ccgatggtga ttatatcatc
     6781 acaggaacaa aaaataccgg tgctttcttt gatctttatt caccaatgat cacacttgtg
     6841 cagggtggat ttttgtggag ttcaacactt tcgtttacac caacattatc acaagcaacc
     6901 ggtggcgcca tcacccaaac aacagaaggt ggatataaat ggatacgcta tcaacaaaac
     6961 ggaacttatt tcgatcctgc tgttggcgat tatgtagaag tggcaacatt agacatgtgg
     7021 cttaccgaaa cagcaacaca tactatcaac tatgcacctt ataatacttt cgagtgtatc
     7081 gcagcactgg cgggcactcc ggcaattgac gatgcttatg tgattggcgg ttctgcatgg
     7141 acaattggag ttccggcata caatctcgtg ataggcaccg gcgctgctga tgcaatgcct
     7201 gcatgtatat ttaattgtgt gtggccgggt gatgcagata attccggatt ggtagatatg
     7261 gatgatcttt tatctatcgg acttggattt ggaaataccg gagctgctcg agatgctatt
     7321 gatatttcgt ggactgcaca tcaggcagaa gcgtgggcaa cagctttacc gggtggtatt
     7381 aatcataaat ataccgattg caatggcgat ggaattattg atgcaacaga tgaagatgcc
     7441 gttgatataa attatggatt tgatcatgag gtaaatacca tgcgcatgag cgaaggtgat
     7501 gtgccgttat attatgctcc cgttgatcca attgtaattg gtgaaaatgc aataccaatt
     7561 atgttgggtg atgtagctac gcctgttgat gcaatttatg gtttgcgatt taccgctaca
     7621 gtcgacggtg aatctatcga cgcatcatct gtttatgtaa catttaatga ttcatggatt
     7681 ggaaatactt ctgagatgtt gcagttatca aaaacacttg caacacctgc tgccgatgcg
     7741 ggacttgtga aaacaacaaa tgaaaatgca aatggttatg gtgaaattgg aacattgcat
     7801 tttgttgtca tcgataatat tgcaggaaaa attacaagcg atgaaataac aatcggattt
     7861 gctgatgcac gcgcaattaa tgtcaacaac gaagacctca gtcttaatac tagcgaacta
     7921 accgtagatg taacattagc aactaataat ccatcgcaac ccgctatttc catttacccg
     7981 aatccggtaa gcaataacct catctacact aaccttgaac ttgtaaacgc tcgcgttacc
     8041 atcagtgata tggtaggacg tgaaatcgtt ttggagcaag ttaatggcaa taccattcaa
     8101 ctgcctcaac tagctaccgg caactatctg atgcgtatta ctcagtctgg tggaaccagc
     8161 gtgttcacat tgagtttgca ataaatcttc ctgtagtttt ggagacaatt acagggcccc
     8221 gtttcttaat tgagacgggg ttttctattt tatggagttt taatgtaatg taaaatattg
     8281 aaaatcaaca tattatgtaa taattgatgt ctaatttatc gctataatcg aatttttttg
     8341 ttctttattt ttgctattag ggtagtgata tttgtatctg taaaattgtc tccaaaactc
     8401 tttaacttaa cactatgaaa ttacgtttta ccctaatcgc cttttcagcg ctgttgttga
     8461 taggcaaccg tgtttccgca caagcatggg gttattcgaa tattggccac tgggtttctg
     8521 acatgtccgt tggttcagat ggttctgtat ggactgttgg ttatggcgga gatgatccgg
     8581 catggtacga tgttgcggcg gattatgtag gaaaggtaaa tccggatggc acatcggcat
     8641 tgggttttca tatggcaatt ccgggtgcaa caacggtata taatttttct attggcgcaa
     8701 caactgaagg cggagctgcc gtattgagct ctaccgatat cctcggttgg gctgttacca
     8761 aactcgatga atccggtgca attgaatggg tgaattcagg gtttgaatca tattatatct
     8821 atggcggtat tctggctgta ttgaatgatg gtaaagttgt ggttggcggt ctggctgatt
     8881 tcgaacatcg cctttatgaa ttttcatcta ccggtgatct catcggttcc tataccattg
     8941 cacctgatac cggtgctgta tggatggtag gatattataa ttttaaagaa gccggtatca
     9001 ccgcaactgc tgatggtgga tttgcttatg ccaccggtgg cgaaaataac agactgctgt
     9061 ataaattcga cagcgacatg aacttagagt ggactggcga ttatccttgg gaaatggaat
     9121 gggaatatcc tatgcaaaat ggattaaaaa ccacaagcga tggcggcttc ttattttccg
     9181 gttcatctac aagtgctgcc acatttgatt acaatggtac cgttcgcaag atagctgcag
     9241 atggcactct tgagtggttt aattcttatt tccatggctc gccatacgaa gagggcactt
     9301 gggccgtaga acttggatca ggtgatatct ctgtgtttac acaagatgcc ggcgaagcaa
     9361 gtacaaatgc ttggatgact actgctacag gtactgaata cggcgctgaa tttattcccg
     9421 taatgaatgt gatcggtggt tattctgaaa caggtgtcga tgtttgggat attgaaaatg
     9481 ctgctgatgg cggtttatat attgcaggta gaatgtggct cgaaaattac gaccagcgtc
     9541 agtttg
//
LOCUS       DAOQOU010000129         9496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3849_length_9496_cov_5.817498, whole genome shotgun sequence.
ACCESSION   DAOQOU010000129 DAOQOU010000000
VERSION     DAOQOU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_9496_cov_5.817498"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..760
                     /locus_tag="PLM90_10770"
     CDS             83..760
                     /locus_tag="PLM90_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89902.1"
                     /translation="MALKLSAEAFEKAISMFEIAHEKSGGYEDYSFRIGLCHERLNNF
                     KSARFQYRKATRQDPFMHEAFFRIGETYRLEERLEPALVNYKKALQVDDTNEDYICTI
                     ISIYKMLDREDDVLEHLRILVNVRADILSYWLDFLIYLFDIGQFEKTIEIADEAIRRS
                     GHFAEYYYIQAAALSALGDGKSALVLLEHALTIDYGRHTILYELNREIALLPAFQQVI
                     LQFAPRS"
     gene            820..1578
                     /locus_tag="PLM90_10775"
     CDS             820..1578
                     /locus_tag="PLM90_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HMZ89903.1"
                     /translation="MNFNLTRLPERTSKPRKEGLCMVMDKGLSVREAEDLLSVSEPYI
                     DLIKLGFGTAFVTPNIENKIAVYKNAGIPTYFGGTLFEAFLVRGQFEDYLRLLEKYKM
                     SYAEVSDGSIELNHDEKCGYINRLSKHVTVLSEVGSKDENKMIAPYKWIELMKKEIEA
                     GSYKVIAEARESGTVGIYHSKGEVRSDLIDEILTQVPQDQIIWEAPLKGQQVYFIKLL
                     GANVNLGNIPPNEVIPLETLRLGLRGDTFHHFLT"
     gene            1589..2245
                     /locus_tag="PLM90_10780"
     CDS             1589..2245
                     /locus_tag="PLM90_10780"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HMZ89904.1"
                     /translation="MEFLHTFIDTFLHLDQYLESWIDTYQNWIYVILFAIIFIETGIV
                     IWPWLPGDSLLFAAGAFCAIGSLNIFIIIPLLFVAAVLGDTSNYFIGKYFGERAMRVK
                     WRGKPILKPEHLAKTHAFYEKYGAKTIVLARFVPIVRTIAPFVAGMGTMTYKTFFSYN
                     IIGAILWIPSITVLGYIFGQNEFVKHNFEIVVLAIIFISVLPIFYELFKARFKRSKNN
                     "
     gene            2257..3015
                     /locus_tag="PLM90_10785"
     CDS             2257..3015
                     /locus_tag="PLM90_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMZ89905.1"
                     /translation="MKHFGLIGFPLSHSFSETYFREKFSREEIEDCIYELFPLENIED
                     VRLLFEVNKDLVGLNVTIPYKESVIEYLDDLDPVAQKIGAVNCIHIDEIQRTGYNTDW
                     MGFRDAIKPFLNKPHQHALVLGTGGASKAVVYALRELGLHVTLVSRSSGNKKIAYGDI
                     TSDIIAHHTVIVNCTPLGMYPNTNECPDIPYDLITDQHLLFDLIYNPGETLFLQKGKA
                     KGATISNGLEMLRLQAEYAWEIWNQQSEESEAEM"
     gene            complement(3086..4003)
                     /locus_tag="PLM90_10790"
     CDS             complement(3086..4003)
                     /locus_tag="PLM90_10790"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ89906.1"
                     /translation="MLKKEFIKFSTSGLVMLLLTTHLYAQPITLSREIGANLGFSSFL
                     GDLGGSDDVGRAFWWDIDPQITRPALGFVYRQEIIPRTAFRFNAYASILKGDDALTDN
                     EFRHYRNLSFRSPIVEASGSVELSAFRFTGVKKKKWTPYIYGGVGAFYFNPQAQYNGE
                     WVNLQPLGTEGQGLPEYPDRQKYSKIAVCFPVGGGFRYITDRDWVLGFEMAARFTNTD
                     YIDDVSGYYPNPDYYYLHYDLETALMAATLSDPSDHTIPEWTQPEYGRGDPTNNDSYI
                     FGGMLTFTYHMEFQRKVNTIKCYFNQDNK"
     gene            complement(4115..5239)
                     /gene="rffA"
                     /locus_tag="PLM90_10795"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(4115..5239)
                     /gene="rffA"
                     /locus_tag="PLM90_10795"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HMZ89907.1"
                     /translation="MIPFNKPSLIGSEHAHMSAAFETGKLSGNGLYTHQCHHYFEQHF
                     GVLRALLTHSCTGALEMSALLCNIKPGDEVIMPTYTFVSTANAFALRGAMIKFVDSKP
                     DHPVMQVEDIEPLVNEKTKAIVVVHYGGYSVDMQPVLDLAIRKGLFVVEDAAQAIGSR
                     YHGVLCGTIGHFGAFSFHETKNVHCGEGGLLAVNDERYADRAEVIWEKGTNRSAFFRG
                     EASKYEWMDLGSSFLPSELQAAWLWGQLEHYSGIRSQRAVLWQAYREPLMEMQKAGYF
                     ELLEEQAGSTHNHHLVAVICKDSIERGKLIQFLEKQGVMAVSHYLCLHKSPYYSEKHD
                     GREMPNALRFERNLLRLPLFHDLSLSGVEKITEVLKSFYA"
     gene            complement(5236..5976)
                     /locus_tag="PLM90_10800"
     CDS             complement(5236..5976)
                     /locus_tag="PLM90_10800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ89908.1"
                     /translation="MEQTPEISVISPVYNSEGNVPELVQRLHEVLGAITPLYEIILVD
                     DFSNDLSADVIAKMREEDDRVKGIFHHQNIGQHPSILHGLMNSSGNVCIVMDCDLQDP
                     PEAITQMFMALGPTDDAVFALRKKTYNSAYQRYLSKLFYISLSLMSGNRLPGEIANYG
                     IYRRAVISKLFDARYRYFLFPLAVRKYARQSKTFEVEHARRFSGKSTYTIGKAGKLAI
                     TAILANSIFGFTFRKRSAQEIVSDNHAI"
     gene            complement(5993..6304)
                     /locus_tag="PLM90_10805"
     CDS             complement(5993..6304)
                     /locus_tag="PLM90_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HMZ89909.1"
                     /translation="MSDAKEKNQLNIELSEEIAEGTYSNLAIITHSSAEIVIDFIRVM
                     PGVPKAKVKSRILMTPQHAKRLLRALSDNINKYESQYGDISDNEGFGGIPMNFGPTAK
                     A"
     gene            6383..8080
                     /locus_tag="PLM90_10810"
     CDS             6383..8080
                     /locus_tag="PLM90_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMZ89910.1"
                     /translation="MKMLPKLLVLTAMLATIGWLSSCRHEAGAEEEGTDLEDLSQLKP
                     VFYPLHIDSQSIAVFLQQYPLFDFHREEVFAFYKQRNDQSAWFNEYGIVDQGGYFMNL
                     LDHFSEEGLRDSVIYYSTLNRMYHTISDPAYSYNGADSLTTQFELMLTSTFFVYAQKV
                     WYGLSEKTTRSLDWYVTRKTVPSVSILDSILSGNASAFTAYEPLFPQYSLLKNALKKY
                     HAIENEPWDSIAIPADRKSILLGDDLPVLSEIKRRLFLLGDLNTSDSTTLYDSTTLAA
                     VMMFQERHGLFADGSMGPKFFTAINRTPAELIRRIEINMERCRWIPDQPGGDYILVNI
                     PEYTMHIYEADTQAWEMNVVVGKSSTSTTIFNDELEYIVFSPYWVPPPSILNNEILPS
                     LKKDPNYLQKQNMEAFNPSTREVINVSKVDWSKYTSMPYSIRQKPGNSNALGWVKFLF
                     PNEHNIYFHDTPSRDLFVRESRGFSHGCIRLQEPKKLAQYLLRNDSTYTEQVIDSLYY
                     LGRENYVKLENKIPVYIIYLTAWVDDNGLVQFRKDIYGHDAKLEQTMYGAPEVDLAN"
     gene            complement(8205..>9496)
                     /locus_tag="PLM90_10815"
     CDS             complement(8205..>9496)
                     /locus_tag="PLM90_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMZ89911.1"
                     /translation="MLISNNIPYGSILSVSDGDEMKKGDVMCSWDPFNAVIISEFAGF
                     VSYEHIIEGITYREESDEQTGHREKVISESRDKTKIPTLIIKDKKGNEVKGYNLPVGA
                     HIAVEEGEEVKSGQTIAKIPRALGKVRDITGGLPRVTELFEARNPSNPAVVAEIDGVV
                     AFGKIKRGNREIEIESRDGEKRKYLVPLSKQILVQENDYVYAGMPLSDGAITPSDILA
                     IKGPYAVQEYIVNEIQEVYRLQGVKINDKHIETIVRQMMRKMTITDAGDTRFLEDSSV
                     DKLDFLEENDRIFDKKVVTDAGDSTKLKAGQIVTLRQLREENSQLKRADKKPVEYRDA
                     IAATATPLLLGITRASLGTQSWISAASFQETTKVLSTASISAKTDYLMGLKENVIVGH
                     QIPAGTGQRELLDLVIGSKEELDELNAAKKRKNNDGE"
BASE COUNT         2809 a         2086 c         1835 g         2766 t
ORIGIN      
        1 gggaccaggg catgttgtag gtgaaatatc actcatttta agaaggccag cgaatgcaag
       61 cgtgactgca acgcatccaa ccgtggcgct taagctatcg gcagaagcct ttgaaaaagc
      121 aatttctatg tttgagattg cgcatgaaaa gtccggagga tacgaagatt acagcttccg
      181 aataggctta tgccacgagc gcttgaacaa tttcaagtcg gcccgcttcc aataccgcaa
      241 ggccacgcgc caggatcctt tcatgcacga ggctttcttc cgtattggag aaacctatcg
      301 cctagaagaa cgcttggaac ccgccctggt gaactacaaa aaggcacttc aggttgacga
      361 taccaatgaa gattacatct gcaccatcat cagcatatac aaaatgcttg atagagagga
      421 tgatgtgctt gaacatcttc gtatcctggt gaatgttcgt gctgatatcc tcagttactg
      481 gctcgatttt ctaatttatc tgttcgatat aggtcaattc gaaaaaacca ttgaaatagc
      541 tgatgaagcc atacgaagaa gcggtcattt cgccgaatac tactatatcc aggctgcagc
      601 actatcggcg cttggcgatg gaaagagcgc attggttctg ttagaacatg cacttaccat
      661 tgactatgga cgtcatacca tactatatga gttgaatcgc gagattgccc ttctacctgc
      721 cttccagcaa gtgatcctgc agttcgcacc acgctcttga tcagtaagat atgctgtcta
      781 ttgcgtattt ttgcagctgt aaccaatagt tctaagtaca tgaattttaa tttaacccga
      841 cttccggaac gcacatcgaa accgaggaag gaaggcttgt gcatggtaat ggacaaaggc
      901 cttagcgtaa gagaagcgga agacctcctt tcagtttctg aaccttatat cgatttaata
      961 aaattgggat ttggcaccgc cttcgtaact cccaatatcg aaaataaaat tgccgtttat
     1021 aaaaatgcag gcattccgac ttatttcggt ggtacattgt ttgaagcatt tttagtgcgc
     1081 ggacaatttg aagattatct gcgtctgctc gaaaaatata aaatgtcgta cgcagaagta
     1141 agcgacggtt caatagaatt gaatcacgat gaaaaatgcg gctacatcaa caggctttct
     1201 aaacacgtta ccgtattaag tgaggtagga tcaaaagatg aaaataaaat gatcgcacct
     1261 tacaaatgga tcgagttaat gaagaaagaa atcgaagcag gaagctataa agtaattgca
     1321 gaagcacgcg aaagtggaac cgttggaatt tatcattcta aaggtgaagt gcgcagcgat
     1381 ttgattgatg aaattcttac acaagttccg caagatcaga ttatttggga agcaccttta
     1441 aaaggacaac aagtgtactt cattaaactg ctcggcgcca acgtaaatct tggaaatatt
     1501 ccacctaacg aagttattcc tcttgagaca ttgcgtcttg gacttcgcgg tgataccttc
     1561 caccatttcc tgacgtaatc tatcctgaat ggaatttctg cacacgttta tcgatacttt
     1621 cctgcacctt gatcaatacc tggaaagctg gatcgatacc taccaaaact ggatatatgt
     1681 tatcctgttt gctatcattt ttattgaaac aggtattgta atctggccct ggttgccggg
     1741 tgattcatta ctttttgcag caggtgcatt ttgtgcaatc ggatcgctga atattttcat
     1801 catcatacca ttgctatttg ttgccgcagt acttggcgat accagcaatt atttcatagg
     1861 aaaatatttt ggtgaaagag ccatgcgtgt aaaatggcgc ggtaagccga ttctgaaacc
     1921 ggaacacctc gcaaaaaccc acgctttcta tgaaaaatat ggcgctaaaa ctatcgtgct
     1981 cgctcgcttt gttcccattg tgcgcactat cgctccattt gttgcaggta tgggaaccat
     2041 gacctataag acattctttt catacaacat tatcggcgca atactttgga ttccttctat
     2101 cacagtatta ggatatattt ttggacaaaa cgaattcgtg aaacacaact ttgagatagt
     2161 agttttagcc attatcttca tttctgtgtt gccaatattt tatgagctgt tcaaagcgcg
     2221 atttaaaagg tcgaaaaata attaatcatt tgacctatga aacacttcgg gctgatcgga
     2281 tttcctttat ctcattcttt ttctgaaaca tatttcagag agaaattttc acgggaagaa
     2341 atagaggatt gcatatacga attatttcca ctcgaaaata ttgaagatgt gcgtttactg
     2401 tttgaagtaa acaaagattt ggtgggattg aatgtcacta ttccatacaa agaaagtgtt
     2461 attgaatatc tcgatgatct tgatcccgtt gctcaaaaaa tcggtgcagt taattgtatt
     2521 catatcgatg aaattcaacg caccggatac aataccgact ggatgggatt tcgagatgca
     2581 ataaaacctt tcctgaacaa acctcatcaa catgctttgg ttttgggaac aggtggtgca
     2641 tctaaagctg ttgtttatgc cctccgtgaa ttaggattgc atgttacact cgtttcccgc
     2701 tcttcgggga ataaaaaaat tgcttatggc gatataactt ctgatattat cgcacatcat
     2761 acggtgattg taaattgcac accacttggc atgtacccaa atacaaacga gtgccctgat
     2821 attccttacg atttaatcac tgatcaacac ttgctgtttg atttgattta taatcccggg
     2881 gaaacattat ttcttcaaaa aggaaaagct aaaggtgcaa ccatcagcaa cggacttgaa
     2941 atgttgcgac tgcaagcaga atacgcctgg gaaatatgga atcagcaaag tgaagaatcg
     3001 gaagccgaaa tgtaattggc acgaatcaac ataatggaaa ttgctttcca tcgcccaata
     3061 attgaaagga aaatctgctt aaattttatt tgttatcctg attgaaataa catttgatcg
     3121 tattcacctt acgctgaaat tccatgtggt aggtgaatgt gagcatgcct ccaaaaatgt
     3181 atgagtcatt attggtagga tcgccgcgac cgtattcggg ttgagtccac tcaggaattg
     3241 tatgatcgga tggatctgat aatgttgccg ccattaatgc ggtttcaaga tcataatgca
     3301 aataataata atccggattg ggataataac cacttacatc gtcgatataa tctgtattgg
     3361 taaaacgagc agccatttca aaacccaaaa cccagtctct atcagtgata tatctgaatc
     3421 ctcctccaac aggaaaacat actgctattt tcgaatattt ctgacgatca ggatattcag
     3481 gtaatccttg tccttcagta cctagtggct gaagattcac ccattcgcca ttgtattgtg
     3541 cctgcgggtt aaaataaaat gcaccaacac ctccatagat atatggcgtc catttctttt
     3601 tcttcacacc ggtaaatctg aaagcactta attctacaga accacttgct tcaacaattg
     3661 gactccggaa gcttagattt ctataatgac ggaattcatt atccgtcaat gcatcatccc
     3721 cttttaaaat ggaggcataa gcattaaaac gaaaagcagt tcttggaatg atctcctgac
     3781 gatacacaaa accaagtgcc ggtctggtaa tctgaggatc aatatcccac cagaaggcgc
     3841 ggccgacatc gtcagaaccc ccaagatctc cgagaaaact cgaaaaacca aggtttgctc
     3901 caatctcacg agaaagcgtg atcggctggg catataagtg tgtggtcagc aagagcataa
     3961 caagccctga cgtagagaat tttatgaatt ctttttttaa cattcgggtc tgttgcttgc
     4021 tagggcgata cgaaactcag cattttttta acaaattcaa aatattagat atgtgacatg
     4081 aaaaaattca tgctgaaatc atatttcacc cagattatgc ataaaacgat tttaacactt
     4141 cagttatttt ctccacccca ctgaggctta ggtcatggaa caacggcaaa cgcagcaaat
     4201 tccgctcaaa cctaagtgca ttgggcattt cacgaccatc atgcttctcg gaatagtacg
     4261 gtgacttatg caagcacaaa taatgtgaaa ctgccataac tccctgtttt tcaagaaatt
     4321 gtatcaattt ccccctttct atagaatcct tacaaattac agctacgagg tgatgattat
     4381 gtgtgcttcc cgcttgctcc tccaataatt caaaatatcc ggccttctgc atttccatca
     4441 gaggctcccg atatgcctgc cataacactg ctctttgtga cctgattccg gaataatgct
     4501 ccaactgccc ccaaagccac gcagcttgta actcagaggg caaaaaactc gatcccagat
     4561 ccatccactc atatttggag gcctcccctc tgaaaaatgc cgacctgttt gttccttttt
     4621 cccaaatcac ctccgcccta tctgcataac gctcgtcgtt cactgctaat agtcctcctt
     4681 ctccgcaatg cacatttttt gtttcatgaa aactgaatgc accgaaatgc cctatcgttc
     4741 cacataatac accatgatac ctgctgccta tagcttgtgc ggcatcctcc actacaaaca
     4801 aaccttttct aattgccaaa tcaagaaccg gctgcatatc aaccgaataa ccaccataat
     4861 gcacaactac tattgctttc gtcttttcat tcaccagtgg ttctatatcc tcaacctgca
     4921 ttaccggatg atcaggctta ctatctacaa attttatcat cgcacctctt agcgcaaatg
     4981 cattcgctgt tgaaacaaat gtatacgttg gcataatgac ttcatcaccg ggcttgatat
     5041 tgcacagcaa tgccgacatt tctaatgccc cggtgcagga atgcgtcaac aaagcgcgaa
     5101 gcacgccaaa atgttgttcg aaataatgat gacattgatg tgtatacaaa ccatttcccg
     5161 acagttttcc ggtttcaaat gcagccgaca tatgtgcatg ttcgctgcca attaaagagg
     5221 gtttgttaaa tggaatcata tcgcatgatt atcgctcaca atttcctgcg cggaacgctt
     5281 cctaaaagta aaaccaaaaa tagaatttgc caatatcgcc gtaatggcca atttgcctgc
     5341 cttaccaatg gtgtatgtcg atttaccact aaatcgccgt gcatgctcta cttcaaatgt
     5401 ttttgattgc cgtgcatatt tgcgtaccgc cagcgggaat aaaaaatagc gataacgagc
     5461 atcaaacaat ttacttataa ccgcccgacg gtaaattcca taatttgcta tttcacccgg
     5521 aagccgatta cctgacatca gcgacaaact gatataaaat aatttagata aatatcgctg
     5581 gtaggcagaa ttatatgttt tcttcctcaa ggcaaacacg gcgtcatcag tgggacccaa
     5641 tgccataaac atttgtgtta ttgcttccgg aggatcctgc agatcgcaat ccataacaat
     5701 acaaacgttc ccgcttgagt tcatcaatcc atgcaaaata gaagggtgct gtccgatatt
     5761 ttgatggtga aaaatacctt ttactctatc gtcctcctca cgcatttttg caataacatc
     5821 agcactcaag tcattgctga aatcatctac taaaatgatt tcatacagcg gtgttattgc
     5881 acctagtacc tcgtgtaatc gctgcactaa ttccggaaca ttgccttcac tgttatatac
     5941 aggactgata acagaaatct ccggcgtctg ctccataaga atcaattcac gatcaagcct
     6001 ttgcagttgg cccgaagttc atcggtatac caccaaaacc ttcattgtcg gaaatatcgc
     6061 cgtattggct ttcatactta ttgatattat ctgacaaggc acgcaataaa cgctttgcat
     6121 gctgaggtgt catcaaaatg cggcttttca ctttagcctt tggcacaccg ggcattactc
     6181 tgatgaaatc tataactatc tcagcgctgg aatgcgtaat gatggcaagg tttgaatagg
     6241 tgccttcagc aatttcctca ctcaattcga tgttgagttg gtttttttct tttgcgtcgc
     6301 tcataatttc cttttgatgt aaaagtaccc gaaaatgagg gcaatcagaa atatttaaga
     6361 acttcgtaaa aaaattaccg ctatgaagat gctccccaaa cttctggttt tgactgccat
     6421 gctggctacc ataggatggc ttagctcttg ccgacatgag gccggggctg aagaagaagg
     6481 cacagactta gaggaccttt cgcagctcaa accggtattt taccccctgc atatcgactc
     6541 tcaatccata gcggtttttt tacaacaata ccctcttttt gattttcatc gggaggaagt
     6601 atttgccttt tataaacaaa gaaatgacca atcggcatgg tttaatgagt atggcattgt
     6661 tgatcagggc ggttatttca tgaacctgct cgatcatttc agcgaggaag gattgcgcga
     6721 ttccgtgata tattacagca ccctcaatcg catgtatcac accatcagtg atccggctta
     6781 ttcatacaat ggtgccgata gtcttaccac acagtttgaa ttgatgctaa catcaacatt
     6841 ttttgtctat gcgcagaaag tatggtacgg actcagtgaa aaaacaacgc gctcattaga
     6901 ttggtatgtt accagaaaaa ctgttccatc agtttctatt ttagattcca tactcagtgg
     6961 taatgccagt gcattcactg catatgaacc actgttccca caatattctt tattgaaaaa
     7021 cgcacttaaa aaatatcacg caatcgaaaa tgaaccatgg gattccattg ctattcctgc
     7081 agatagaaaa tcaattttgc tcggcgatga tttacctgtg ctgtcggaaa taaaacgccg
     7141 attgtttttg cttggcgatt taaatacatc tgattcaaca acactttacg acagcactac
     7201 actagctgca gtaatgatgt ttcaggaaag acacggcctg tttgccgacg gttctatggg
     7261 acctaaattt tttactgcaa ttaatagaac accggctgaa ttaatcagac gcattgaaat
     7321 aaatatggaa agatgcagat ggatccccga ccagccgggt ggcgattata ttcttgttaa
     7381 tataccggaa tataccatgc acatttatga agcagatacg caagcatggg aaatgaatgt
     7441 ggttgtaggg aaatcaagca cgagcaccac catttttaac gacgaattgg aatacatagt
     7501 gttctcacca tactgggtcc ctccaccgag catattaaac aacgagatac ttccatcact
     7561 taaaaaagac ccgaactatt tgcaaaagca aaatatggaa gcttttaatc cctctacccg
     7621 ggaagtaata aatgtaagca aggtagactg gagcaaatac acctctatgc catatagtat
     7681 tcgtcaaaaa ccaggtaata gcaatgcttt aggatgggtg aaatttttat tccccaacga
     7741 acataatatt tatttccacg acacaccttc acgtgatttg tttgtcagag aatcgcgcgg
     7801 atttagtcat ggatgtattc gtttacagga acctaaaaaa ctggctcaat atttattgcg
     7861 gaatgatagc acctacaccg agcaagtgat cgactcacta tattatttag gcagagagaa
     7921 ttatgtgaaa ctggaaaata aaattccggt atatattatt tacctgactg cctgggttga
     7981 tgacaatggt ctggtacaat tcagaaaaga tatttatggt cacgatgcca aactggagca
     8041 aacaatgtat ggagcacccg aagtagattt agcaaattaa attacttgcc tcgtcctcaa
     8101 attcaatttg gttgttggga cattgaacaa ttcaaaacaa gccagcacca gaaataaaaa
     8161 aagccctccg taaacggaag gctttttatt atttgatgag gtgcttattc accatcatta
     8221 tttttgcgtt ttttagccgc attcaactca tccagctcct ctttgctacc gatcacaaga
     8281 tcaagcaatt cgcgctgacc tgttccggca gggatctgat gtccaacgat aacgttttct
     8341 ttcaagccca tgagataatc tgtttttgct gaaattgacg ctgtgctcaa caccttggta
     8401 gtttcctgga acgacgctgc agatatccag ctctgagttc ccaaagatgc tctggtaata
     8461 ccgagcaaca atggtgttgc agttgctgca atagcatcgc gatattcaac aggtttttta
     8521 tcggcacgct tcaattgtga attttcttca cgcaattgac gcaatgttac gatctgaccg
     8581 gctttcaatt ttgttgaatc acctgcatca gtaactacct tcttgtcgaa gatacgatcg
     8641 ttctcttcaa ggaagtcgag tttatcaact gatgaatcct caaggaatct agtatcacct
     8701 gcatctgtga tggtcatctt acgcatcatc tgacgaacaa tcgtttcgat atgcttgtcg
     8761 ttgatcttca ccccctgtaa gcgatatact tcctgaattt cgtttacgat atactcctga
     8821 actgcgtatg gacctttgat ggcaagaatg tctgaaggtg taattgcacc atccgacaat
     8881 ggcataccgg catatacgta gtcattttcc tgaacaagaa tctgcttaga aagtggcacc
     8941 aggtatttgc gtttttcgcc atcgcgactt tcgatttcga tttcgcggtt accacgtttg
     9001 atttttccga aggcaacaac tccatcgatc tctgcaacaa ctgcagggtt tgatgggtta
     9061 cgtgcttcaa acaattcagt tacacgtgga agaccacccg tgatatctcg tacttttccg
     9121 agtgcacgtg ggatcttggc aattgtttga cctgatttca cttcttcacc ttcttcaacg
     9181 gcgatgtgcg cacctacagg aaggttatat cctttaacct catttccttt tttatcctta
     9241 atgataagtg taggaatttt ggttttatcg cgagactcag aaatcacttt ttcgcggtga
     9301 cctgtttgct catcactttc ttcgcggtag gtgatacctt caatgatatg ctcgtaagaa
     9361 acaaatccgg cgaattcaga aatgataaca gcgttgaatg gatcccaaga acacattaca
     9421 tcacctttct tcatttcatc accatctgaa actgaaagta ttgaaccgta aggaatattg
     9481 ttagagatca acatct
//
LOCUS       DAOQOU010000130         9399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3917_length_9399_cov_5.771832, whole genome shotgun sequence.
ACCESSION   DAOQOU010000130 DAOQOU010000000
VERSION     DAOQOU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3917_length_9399_cov_5.771832"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLM90_10820"
     CDS             <1..911
                     /locus_tag="PLM90_10820"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMZ89912.1"
                     /translation="DTHVMGFIKLGAHFDVNTGAISADKLKGTYMLLDEASVTGTANI
                     LMAAVLAEGKTTIYNAACEPYLQQLCDMLVRMGAKITGIGSNMLHIEGVSTLGGCEHT
                     ILPDMIEIGSFIGLAAMTQSEITIRDVHFEQLGIIPEVFSRLGIAMERRGDDLFIPAQ
                     EHYEIQNFIDGSILTIADAPWPGFTPDLISIVLVVATQARGSVLIHQKMFESRLFFVD
                     KLIEMGAQIILCDPHRATVIGLDRKFRLRGLEMSSPDIRAGVALLIAALSAEGKSIIR
                     NIEQIDRGYQLIDQRLNNIGAQIVRI"
     gene            1047..1592
                     /locus_tag="PLM90_10825"
     CDS             1047..1592
                     /locus_tag="PLM90_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ89913.1"
                     /translation="MSKHYFRATAIGGYILLFVMAIVLNSCKTPAPVVGDHIGNIAPE
                     ISAPDAQGKTQSLSALQGKLVLVEFWDSNNSASRKNHFEMQRMYSKYRNTSLDGGNGF
                     AIYSISIDTDPKKWQEAIAADGINFTTMVNDTQAWNSKAVLDYNIASLPKYFLIDDKG
                     IIINHNILIPELEKILIEQAN"
     gene            1594..2178
                     /locus_tag="PLM90_10830"
     CDS             1594..2178
                     /locus_tag="PLM90_10830"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ89914.1"
                     /translation="MDSRLTSSHFTTSSIVYFFAALITIGFLSYGCSNSVTSPTIGVA
                     TGNHSPEIQSENLKGEMISLSAHKGSYVLVEFWDSQNSEARRNHFEMQRIYQKFKDAT
                     FDNGKKFCIYSVSLDTDREKWAKAVAEDNMSWPCNTIDQRAWNSPSVLAYNISYLPKY
                     YLIDGDGVIIRRNIMINELEALLNENLQGRLSNS"
     gene            2300..2860
                     /locus_tag="PLM90_10835"
     CDS             2300..2860
                     /locus_tag="PLM90_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMZ89915.1"
                     /translation="MSETEQNKDQEVKNQAEQDVETPVNEQENLPVDEGAVDDATTMV
                     KELGEQKDKYIRLLAEFENYKRRTTKERLELFKTAGLEVIKDLLPALDDIDRAENAIA
                     SSKDIEAVKDGLKLMSDKLRNTLSQKGLKEIACKGLPFDTDTMEAITEIAAPTPELQG
                     KVVDVLEKGYTLNEKIIRYAKVVIGK"
     gene            2898..>3993
                     /gene="dnaJ"
                     /locus_tag="PLM90_10840"
     CDS             2898..>3993
                     /gene="dnaJ"
                     /locus_tag="PLM90_10840"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMZ89916.1"
                     /translation="MAKRDFYEILGVGKQASAEEIKKAYRKMALQYHPDKNPGNAEAE
                     EKFKEAAEAYEVLSDQQKRARYDQFGHAGMGGAGGGAPHMNMDDIFSQFGDIFGESVF
                     GDFFGGGGGGRRQQRQRGVRGSNIRIKVKLTLKEIAEGARKTIKVKKYVLCDVCHGSG
                     AKDASSVTTCKTCQGSGYVRRVQNTILGQMATTQTCHTCHGSGQQVTANCTKCHGEGR
                     MYGEDTITLDIPAGVHEGVELSMNGKGNAGEKGGPNGDLLISVEEVKDPDLKREGNHV
                     LYDLYISFADAALGTKVEVPTIDGKVRVTIPEGTQGGHIMRLKGKGLPGLNSYSKGDQ
                     IIQVHIWVPKKLSSEERKMMEKLQESKNFIP"
     assembly_gap    3994..4093
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4377..5777)
                     /gene="hisS"
                     /locus_tag="PLM90_10845"
     CDS             complement(4377..5777)
                     /gene="hisS"
                     /locus_tag="PLM90_10845"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMZ89917.1"
                     /translation="MSKQKPSIPKGTRDFGPDEVAKRNYIIGTIRKVYELYGYQPLET
                     PVMENLSTLMGKYGEEGDKLLFKILNSGDYLKDADTQLLSEKNSNKVLSAISEKGLRY
                     DLTVPFARYVVMNQNEISFPFKRYQIQPVWRADRPQKGRYREFYQCDADVIGSNSLLL
                     DAEMVMMIADVFSKLGLRVTIRLNNRKILDGLNEYIGETEKNVALITAIDKLDKIGKE
                     GVQNELREKGFTDDQISKTENILAITGSATDKISALEKLFSGEKGKKGISEIKELFAK
                     IANVGSSNIQIAFDLTLARGLSYYTGTIYEVVSNDVQMGSILGGGRYDDLTGIFGLPN
                     MSGVGISFGLDRIYDVITELNKFPESATLQTQVLFIAFDEAAETAALQYARSLRDAEI
                     RCELYPDVQAKTAKKFKYADDKKIPYCAIIGSNEMAEQKVMLKNMQSGDQQLLSIEEV
                     IEIITGIRSNFVDLSE"
     gene            complement(5819..6280)
                     /locus_tag="PLM90_10850"
     CDS             complement(5819..6280)
                     /locus_tag="PLM90_10850"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HMZ89918.1"
                     /translation="MKVLMVCLGNICRSPLAEGILRHKSQEKGLNWHIESAGTGAWHV
                     GQAPDPRSQRVAKQHGIDISKQRAQQFSPYHIEMFDLIFAMDSSNYQDIIRHAKTDAE
                     KNKIQLIMNMVEPGMNKAVPDPYYDDHLYATVYQMLDEACDNIISKYAINN"
     gene            complement(6315..7109)
                     /locus_tag="PLM90_10855"
     CDS             complement(6315..7109)
                     /locus_tag="PLM90_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89919.1"
                     /translation="MKYITLACMLCLSGLNVFAQFKPGDIGYNVNSEIIFKNAHVSSQ
                     IATVVESEDMSFVVNYNYYNSNGQLDSTVYPPDYNVDQDEQFPQMKDIYTYLPDGKIS
                     MITMKGYDMFDINYGFEYNKKGKLITSLVASAEAREYTYTYDNKGRISERNGKGARFV
                     YENDEDTDGKLVMIDIERSTYTWNPNGDLSEVNFYYMNEWMQRTVYSYNEDHQVTRMD
                     VYNDNGPDANPSFYISYQYNNQGLLTESTTVESDYTGKTTYAYTYY"
     gene            complement(7130..7888)
                     /locus_tag="PLM90_10860"
     CDS             complement(7130..7888)
                     /locus_tag="PLM90_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89920.1"
                     /translation="MPSSDVNGFNKTMGLIVAFIGLPVCFIGLTGYIAIGRHGMGWIF
                     LILALSGITIIAGGVQMFFQGRADAAKQREVLQAAKAGKAESQITKIITEWPIDDKTW
                     ADFCINEKHYRRGDNYIFFFMFLAIGSFILVASRGSGLLLAVCISGVIGLITVILRYN
                     VAMKKVHTISGIEKKVIIGKRYIIMNGILYPINNERMTTHKIQLITTSIPAILEFTIY
                     WPTRSGETFDELRIPVPDEAISNAKLVVNSFTSN"
     gene            7920..9296
                     /gene="mnmE"
                     /locus_tag="PLM90_10865"
     CDS             7920..9296
                     /gene="mnmE"
                     /locus_tag="PLM90_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMZ89921.1"
                     /translation="MFVADHKDTIVALSTAPGVGAIAVIRMSGDKAIDIMTAVFKGKN
                     LAEAKANTLHFGRIESETTIIDEVVVGIFRAPRSYTGEDIIEVSCHGSPYIQQQILTL
                     LIAKGARMAKPGEFTMRAFLNGKMDLTQAEAVADLIAAQSQASHDVAIKQMRSGYGDA
                     LKNLRYELIHFASLIELELDFSEEDVEFAERSHLMHLLEQINVELDRLIKSFSLGNVI
                     RNGVETVIAGRPNAGKSTLLNALLNEDRAIVSEIAGTTRDTIEAELNIKGVRFRLIDT
                     AGIREAKDEIEKIGVAKTMEKIKTATLLIYVFDVHTMTPEEVGADLDSLVSSGDNMLV
                     IANKIDAAGGQERAALFTKVNPDGFISSLQKVNIERLKDAMFARIVHEDITLEDAVVT
                     NARHLEALRKARATMTEVQDAITHKTSGELTALSIRYALNAIGEITGEVTNDDLLDNI
                     FRKFCIGK"
BASE COUNT         2701 a         1819 c         2051 g         2728 t
ORIGIN      
        1 tggataccca tgtaatggga ttcattaaac tgggagcaca ttttgatgta aacacaggtg
       61 ccatcagcgc tgataagttg aaaggtactt atatgttgct tgatgaagca tccgtaaccg
      121 gtacagctaa tattttaatg gctgcagtat tggctgaagg caaaactact atctacaatg
      181 ctgcctgcga accttatctg cagcaactct gcgatatgtt ggtgcgcatg ggagcaaaga
      241 ttacgggcat aggctcaaat atgttgcata ttgaaggggt gagtacctta ggtggatgtg
      301 agcatactat tcttcccgac atgattgaaa tcggcagttt tatcggactg gcggcaatga
      361 cacagagtga aatcaccatt cgcgatgtac attttgagca acttggtatc atcccggaag
      421 tgttttcaag attgggaatt gccatggaac gccgtgggga tgaccttttc atacctgctc
      481 aagaacatta cgaaatacag aatttcatcg acggatcaat acttaccata gccgatgcac
      541 cttggccggg atttacgccc gaccttatca gtatcgtttt ggtggtggct acgcaagcaa
      601 gaggaagcgt gcttattcat cagaaaatgt ttgaaagcag attgttcttt gtcgacaagc
      661 tgatagaaat gggtgcgcag atcatcttat gcgacccgca tcgggcaaca gtaatcggtt
      721 tggacaggaa gttcagattg cgcggactgg agatgagttc tccggatata cgcgccgggg
      781 tggcattgct gatagcggca ttatctgccg aaggaaaaag catcatccgc aatattgagc
      841 agatcgatag aggttatcaa ttaatcgatc agcgattaaa taatatcggc gctcagattg
      901 taaggatttg acggatgacg gcgatttgca tgcgtatgtg ttaattcttg ataatgaccg
      961 gtaataaatt ataattaatt atcggcgcat gattaatttt gtgctcgatt caacggttcc
     1021 cattttcttc acacacaccg tttcagatga gtaaacatta ttttagggct actgccattg
     1081 gtgggtatat actcctgttt gtaatggcaa tagtgctcaa ttcctgcaag acaccggcgc
     1141 cagttgttgg cgaccacata ggtaatatcg cccctgagat atcagctccc gatgctcaag
     1201 gtaaaacgca gtccctaagc gcattgcaag gcaagcttgt attggtggaa ttttgggatt
     1261 ccaataattc agcttcaaga aaaaaccatt tcgagatgca acgcatgtat agtaaatatc
     1321 gcaatacttc tctcgatgga ggcaacggtt ttgcaattta tagcatctca atagatacag
     1381 atcctaaaaa gtggcaagag gcgattgctg ccgacggtat caatttcacc actatggtaa
     1441 atgatactca ggcatggaac agcaaagcag tcttggatta caatattgca tcacttccga
     1501 agtacttttt gatcgacgat aaaggaatta ttattaacca taacatcctg atacccgaac
     1561 tggaaaagat actaatagaa caagcaaatt aatatggata gcagattgac ctcctcccat
     1621 ttcaccacat cgtcaattgt ttattttttc gcagccttaa taaccatcgg gtttctttcc
     1681 tacggatgca gtaattccgt cacctcacca accataggtg ttgctaccgg taaccattca
     1741 cctgaaattc aatcagaaaa tctgaaaggc gaaatgatca gcttgtctgc tcataagggt
     1801 tcctatgtat tggtagaatt ttgggattca cagaattctg aagctcgacg taaccacttc
     1861 gaaatgcagc gtatttatca gaagttcaag gatgccacct tcgacaacgg gaagaaattc
     1921 tgcatttatt cggtttctct ggataccgat cgcgaaaaat gggcgaaagc agttgcagaa
     1981 gacaacatga gttggccatg taatactatc gatcaacgtg cctggaactc tccgtctgtg
     2041 ttggcatata atatcagtta tctacccaaa tactacctca ttgacggcga tggcgtgatc
     2101 attcgtcgca atatcatgat caatgaactc gaagctctcc tgaacgaaaa cctgcaggga
     2161 aggctctcta acagctgatt tggcggtttt ttcgtgtcat tttggcaaat gacagtgcag
     2221 tggcagtatt tttgcccacc ccaattaacc ttttgggaac ggaaggggta tgcatttttg
     2281 acatgaataa ccggcaatta tgagcgaaac agagcaaaat aaggatcaag aagtgaaaaa
     2341 tcaggcagaa caggacgtag aaactccggt aaacgagcag gagaatctgc ctgtggatga
     2401 gggagccgtt gatgacgcta ccactatggt caaggaactt ggcgagcaga aggataagta
     2461 catccgtctc ttggcagaat tcgaaaacta taagcgcaga accaccaagg agcgactgga
     2521 actttttaaa actgcaggct tggaggtaat caaagacctt cttccggcct tggatgatat
     2581 cgaccgtgct gaaaatgcta tcgcctcatc gaaagatata gaagctgtga aagacggctt
     2641 gaaattgatg agcgataagc ttagaaatac actttctcaa aaaggactga aagaaattgc
     2701 atgtaaagga ttgccctttg ataccgatac tatggaggcc atcactgaaa ttgctgctcc
     2761 aacgcctgag ttgcaaggta aggtggtaga cgtattggaa aaggggtaca cattgaacga
     2821 gaaaattatt cgctacgcaa aagtggtgat aggtaaataa taaacgaaaa tcaccacaac
     2881 gaactaccgg cgcaatcatg gcaaaaagag atttttacga aatattaggt gttggcaaac
     2941 aagcttctgc cgaagagata aaaaaggctt atcgcaagat ggctttgcaa tatcaccctg
     3001 acaaaaatcc gggaaatgct gaagctgaag agaaatttaa agaagctgct gaagcatacg
     3061 aagtgttgag cgatcaacaa aaacgtgcac gctacgatca gtttggtcat gccggaatgg
     3121 gtggtgcagg tggcggtgcg cctcacatga atatggatga tatcttcagt cagtttggag
     3181 atattttcgg tgaatcagtt tttggcgatt tctttggcgg cggcggtggt ggaagaagac
     3241 agcaacgtca gcgcggtgtc agaggtagca atatccgcat caaagtaaaa ttgacactaa
     3301 aggaaattgc ggaaggtgca cgcaagacca tcaaagtaaa aaaatatgta ttgtgcgatg
     3361 tatgtcatgg cagcggcgcg aaagatgctt ctagtgtaac tacttgtaaa acttgtcagg
     3421 gatcaggtta tgtgcgcaga gtgcaaaata ccatcctcgg acaaatggca actacacaga
     3481 cttgtcatac ttgccatggt tcaggacaac aggttacggc aaattgtacg aaatgccatg
     3541 gtgaaggtcg catgtatggt gaagatacta tcacgctcga tattcctgcc ggtgtgcatg
     3601 aaggggtgga gttatcaatg aacggaaaag gtaatgcagg ggaaaaaggc ggacccaatg
     3661 gtgatttatt aatcagtgtt gaggaagtga aagatccgga tctcaagcgc gaaggcaatc
     3721 atgttttgta tgatttgtat atcagctttg cagatgccgc actcggaaca aaagtagaag
     3781 tgccaaccat tgacggtaaa gtgcgcgtca ccattcctga aggtacacag ggcggacata
     3841 ttatgcgttt gaaaggaaag ggactgcccg gattgaattc ttattcaaaa ggtgatcaaa
     3901 ttatccaggt gcatatatgg gttccgaaaa aattatcatc tgaagaaaga aagatgatgg
     3961 aaaaactgca ggaatcaaaa aactttattc ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnntctgaag aaagaaagat gatggaaaaa ctgcaggaat caaaaaactt
     4141 tattcccgat gctaatgaga ctaagaagga aaagagtttt tttgataaga tgaaggatat
     4201 gttctgaaag tcttatttag ggagaatatt caggcgagtt aatgaggagt gaccaattta
     4261 aattcggagg agaaaggaga aaggagagag gagagaggag agaggagaga gaagggaatg
     4321 caatagttgc tgactctgcg tctctgcgcc tctgcgtgaa cccaacatta catgtatcat
     4381 tcagacaaat caacaaaatt gctgcggatg ccggtgatga tttctattac ttcttcgatg
     4441 gataataatt gttgatcgcc gctttgcata ttttttagca tcaccttttg ttcagccatt
     4501 tcatttgatc cgatgatagc acaataagga attttcttat catcggcata tttgaatttt
     4561 ttggctgttt ttgcctgcac atcgggatat aactcacaac gaatttccgc atcgcgcaaa
     4621 ctacgtgcat attgcagcgc tgcagtttcg gccgcttcgt cgaaggcaat aaataatacc
     4681 tgtgtttgta aggttgcaga ttcagggaat ttgtttaatt cggtgataac atcataaatg
     4741 cgatctagtc cgaaggaaat acctacgcca ctcatatttg gcaaaccaaa gatacctgtg
     4801 aggtcatcat aacgtccgcc acctaaaatg gagcccattt gcacatcatt ggaaactact
     4861 tcgtagatag tgccggtata gtaacttaat ccgcgagcaa gggtgaggtc aaaagcaatt
     4921 tgaatattgg aagaaccaac atttgcgatt tttgcaaata gttcttttat ttcactgatt
     4981 ccttttttgc ctttctcgcc gctaaataat ttttctaaag cagatatttt atcggtagca
     5041 ctaccggtga tggctagtat gttttcagtt tttgaaatct ggtcatccgt gaatcctttt
     5101 tctcgcaatt cattttgaac accttctttc ccgattttat cgagtttgtc gatagcggta
     5161 atcagtgcaa cattcttttc tgtttcacca atgtattcat ttagtccgtc taatattttt
     5221 cgattgttga gtcgaatggt aacacgcaat cccagtttcg aaaacacatc ggcgatcatc
     5281 atcaccattt ctgcatccaa caatagacta ttgctgccga tcacatcagc atcacactga
     5341 tagaattcgc ggtagcggcc tttctgcgga cgatcagcgc gccataccgg ttgtatttga
     5401 tagcgtttaa atgggaagct gatttcattt tgattcatca ccacgtatcg cgcaaagggc
     5461 actgtgagat cgtaacgtaa acctttttct gaaatagcac tgaggacttt gtttgaattt
     5521 ttttctgaca gtaattgtgt gtcggcgtct tttaaataat cgccgctgtt gagtattttg
     5581 aataataatt tatcaccctc ttcaccatat ttacccatga gcgttgagag attttccatg
     5641 acaggtgtct ctaaaggctg atatccatat aattcataaa cctttcttat ggtgccgatg
     5701 atataattgc gtttagccac ttcatccggt ccgaaatctc tggttccttt tggtatactt
     5761 ggtttttgct tgctcatgaa aattatatta tatcagcctg attgctgtgg taaaattatt
     5821 aattgtttat tgcgtatttc gaaatgatat tatcgcatgc ttcatcaagc atttgatata
     5881 ctgttgcgta cagatgatca tcgtagtatg gatcgggaac tgctttgttc atgccgggtt
     5941 ccaccatatt catgatcagt tggattttat ttttttctgc atccgttttt gcatgtctga
     6001 tgatatcctg ataattgctg ctatccattg caaatattaa atcaaacatt tctatatgat
     6061 acggactgaa ttgctgagca cgttgcttgc tgatgtcaat cccgtgctgc ttggcaacac
     6121 gctgtgatcg tgggtcagga gcttgtccta catgccaggc tccggtgccg gcgctttcga
     6181 tatgccagtt caaccccttc tcctgcgatt tatgacgcaa tataccttct gcaaggggac
     6241 tgcgacaaat attacccaaa caaaccatca gcactttcat gctgcaaaat tacagactat
     6301 tgatcacatg taaatcaata atacgtatat gcgtatgtgg tttttccggt ataatcagat
     6361 tcaactgtag tcgattcagt cagcaaacct tgattattat attggtagct gatataaaat
     6421 gaaggattag catccggccc attatcgtta tatacatcca ttcttgtcac ttgatgatct
     6481 tcattgtagg agtaaactgt gcgttgcatc cattcgttca tataataaaa gtttacttct
     6541 gacaggtcgc cgtttggatt ccatgtgtaa gtagatcgtt caatatctat catcaccaac
     6601 ttcccatcag tatcttcgtc gttttcataa acaaaccttg ctcctttgcc gtttctttct
     6661 gatattcttc ctttgttatc ataggtataa gtgtactccc gggcttcagc acttgcgacc
     6721 aggcttgtta taagtttgcc ttttttatta tactcaaatc cataatttat atcaaacatg
     6781 tcgtaacctt tcatggtaat catgctgatt ttgccatctg gtaaatatgt atatatgtct
     6841 ttcatttggg ggaactgttc atcctgatct acattataat ccggaggata aaccgttgaa
     6901 tctaattgcc cattactgtt atagtaatta taatttacca caaatgacat atcttcagat
     6961 tcaaccacag tggctatttg agatgaaacg tgtgcatttt tgaatattat ttcgctgttt
     7021 acattatagc caatgtcgcc gggtttaaat tgagcaaaca cattcaggcc tgataaacat
     7081 agcatgcaag ctaaggttat atatttcata gtgatatatt ttctttgttt tagttggatg
     7141 taaaagaatt tactaccaat ttggcattac taatagcctc gtcaggcaca ggtatgcgca
     7201 attcatcaaa cgtttctccg cttcgtgttg gccagtaaat cgtaaattcc agaattgccg
     7261 gtatgcttgt tgtgatcaat tgaattttgt gtgttgtcat ccgctcgtta ttgataggat
     7321 aaaggatgcc attcataatg atgtatcgct taccaataat cacttttttt tctatgccac
     7381 ttatagtatg cactttcttc atggcgacat tgtaacgtaa aatcactgtg atcaatccaa
     7441 ttactccgct gatgcatacc gctaacagta aacctgaacc tctggatgca actaaaatga
     7501 agctgcctat ggcaagaaac ataaagaaga aaatataatt atcgccgcga cgataatgtt
     7561 tttcgtttat gcagaaatct gcccaggttt tatcatcaat aggccactcg gttataatct
     7621 ttgtaatttg agattctgcc ttacctgcct tagctgcttg aagcacttct ctttgttttg
     7681 ctgcatcggc tctgccctga aagaacattt gaacgccacc tgcaatgatt gtgatgccgc
     7741 ttaaagccaa gattagaaat atccaaccca ttccatgacg gccaatggcg atatagcctg
     7801 taagtccgat aaaacataca ggaaggccta taaaggctac aatgagcccc attgttttat
     7861 taaatccgtt tacatcagat gatggcatat tcttaaagct tctgtgtacc ttcgcaggta
     7921 tgtttgttgc tgatcataaa gatactatag ttgcgctgag tactgccccc ggtgttggtg
     7981 ctattgctgt gatacgcatg tcgggcgaca aggcaataga cattatgact gcagtattca
     8041 aggggaaaaa ccttgctgaa gcaaaggcta acaccctgca ttttggaagg attgagtctg
     8101 aaactacgat tatagatgag gtggttgtcg gtattttcag agcacccaga tcttacacgg
     8161 gtgaggatat tatagaagtg agttgtcacg gttctccgta tatccaacaa caaatactta
     8221 cgttgttaat tgccaaaggt gcgcgtatgg cgaagcctgg agagtttacg atgcgcgcat
     8281 ttctgaatgg taagatggat cttacgcagg cagaagcagt tgccgatttg atcgcggcgc
     8341 aatcgcaggc atcacatgat gtggcgataa aacaaatgcg atccggttat ggtgatgcat
     8401 taaaaaatct gcgatatgaa ttaattcatt ttgcatcctt gattgaattg gaattagatt
     8461 ttagcgaaga agatgttgaa tttgccgaga gaagtcacct catgcatctg cttgaacaga
     8521 taaatgtaga gctcgacagg ttaattaaat ctttttcttt aggaaatgtt attcgcaatg
     8581 gtgttgaaac tgttattgca ggcaggccga atgcgggtaa gtcgacctta ttgaatgcat
     8641 tattaaacga agatcgtgcc attgtaagcg aaattgcagg aactactcgc gatacaattg
     8701 aagcagaatt aaatattaaa ggtgttcgct ttcgattgat cgatactgcc ggaattcgtg
     8761 aggcgaagga tgaaatagag aagattggtg ttgccaaaac gatggagaaa ataaaaacag
     8821 ccacactgtt gatttatgtt tttgacgtgc atacgatgac acctgaggaa gtgggtgctg
     8881 atttagatag tcttgtcagc agtggtgata acatgcttgt aattgccaat aaaattgatg
     8941 cggcaggagg gcaggagagg gctgcattat ttacgaaggt gaatcccgac ggatttattt
     9001 cttcgctaca aaaagtaaat atcgagcgat taaaagatgc catgtttgcg cgcattgtgc
     9061 atgaggatat cacattagaa gacgctgtag tcaccaacgc ccgtcacctc gaagcccttc
     9121 gcaaggcccg cgccaccatg accgaagttc aagacgccat cacccacaaa accagcggcg
     9181 aactcacggc cctctccatc cgctacgccc tcaacgccat cggcgagatt acgggtgagg
     9241 tgacaaatga tgatttgctg gataatattt ttaggaagtt ttgtatcgga aagtaatatg
     9301 ttttcctatt tcttctaagt gcttgttttt cttaggtttt agcccttttt tctttctctt
     9361 atttgttgcc aaactactct tttttgctta tatttgttg
//
LOCUS       DAOQOU010000131         9349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3950_length_9349_cov_4.445341, whole genome shotgun sequence.
ACCESSION   DAOQOU010000131 DAOQOU010000000
VERSION     DAOQOU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3950_length_9349_cov_4.445341"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1107)
                     /locus_tag="PLM90_10870"
     CDS             complement(208..1107)
                     /locus_tag="PLM90_10870"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HMZ89922.1"
                     /translation="MRKILILLLITGFVSRLEAQELNCAVTVNAEKVTQTDPKIFKTL
                     ESSVFEFMNTRKWTDDAFKPEERIVCQILITVTEELSSDKFRAQASIVSRRPVYGSDY
                     NSTLLNFQDKDFEFQYAEYQALEFNENQFTSNLTAMLAYYAYVIIGLDYDSFAPKGGD
                     KYFLKAQTIVNQASNVDAKGWKSYDGTRNRYWYINNIMDPKFAVMRDAFYQYHRQGLD
                     NLYNDQVKPVSVITKALQTLDNINRTSPNSMIMQLFFSAKSDELLGLYSKATPTEKAK
                     AVSYLTRLDPVNADQYQELLTGK"
     gene            complement(1100..2314)
                     /gene="coaBC"
                     /locus_tag="PLM90_10875"
     CDS             complement(1100..2314)
                     /gene="coaBC"
                     /locus_tag="PLM90_10875"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMZ89923.1"
                     /translation="MSLRGKKVLLGVCGGIAAYKSAYLVRLLTKAGAEVRVMMTPDAA
                     AFVGPLTFATLSKHPVAIDFFDKQTGQWQNHVSTGLWPDVILIAPVTANTIAKLAGGI
                     TDNFLLATTLSSRCPIYLAPAMDLDMWRYQATVDNIAKLKGFGHHIINPVHGELASGL
                     EGTGRMAEPEELLEVMENHFQQVKGPLSGKKVLITAGPTYEAIDPVRFIGNHSTGKMG
                     IALAETCLRLGAEVTLVHGPMQVTCDDADIHSIAVTSAAEMLGACEEAFPACDIFIAA
                     AAVADFTPEAPSAQKIKKGDSQDGSMQLNLRRTTDILGTLSAKKGTRFVVGFALETSN
                     GLEYAKSKLTQKNLDMVVLNTTADRGAGFGHDTNKITLLDKSGEVINFELKSKAAVAQ
                     DIVQHIIRRLHA"
     gene            complement(2317..2661)
                     /locus_tag="PLM90_10880"
     CDS             complement(2317..2661)
                     /locus_tag="PLM90_10880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HMZ89924.1"
                     /translation="MSSLKKINTQLSTQIEVQSLKNIANTSGNIYRSIYIVGRRADQI
                     TGMVRGELHSKLEEFASHQDNLEEIHENREQIEISKYYEKLPKSTIMALDEFLNAKLF
                     FHERETEETKTN"
     gene            complement(2639..3493)
                     /gene="bamD"
                     /locus_tag="PLM90_10885"
     CDS             complement(2639..3493)
                     /gene="bamD"
                     /locus_tag="PLM90_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HMZ89925.1"
                     /translation="MAWTRALALTIISISILVFTGCQYDKLLKSDDFDAKYAKAKEYY
                     GKGDYARALPLLEQMLSVKIGTPEEKEVRYYTAYCYYGQADYFSSSSLFRQVFNIFPL
                     SGEAEEMLFMSAKSMYMASPRYNLDQQYTYSAIEAFQYFVDVFPKSALVPEANKYMDE
                     LRGKLETKLINAAELYYNTEHYESAAITFKNVLLEFPDTRDAEEISYKIADSYFQYAG
                     QSVICKKEERYDDAINSGQDFIQRYPNSPKVADAKDLIEKSTDLKEKAITEITTYKIK
                     CDELSEEN"
     gene            3629..4870
                     /locus_tag="PLM90_10890"
     CDS             3629..4870
                     /locus_tag="PLM90_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HMZ89926.1"
                     /translation="MDIFDKLRKDRGPLGKWSDVAHGYMMFPKLEGEIGPRMMFRGKE
                     RITWSLNNYLGLANHPEVRKIDAEASAQYGLAYPMGSRMMSGNSDHHEHLEKELSSFV
                     QKEDTFLLNYGYQGIMSVIDAICSRHDVIVYDAESHACIIDGVRLHQGKRFVFRHNDI
                     ENCELQLKRAAEMVEGTPGGILVITEGVFGMGGDQGKLKEIVELKKKFNFRLLVDDAH
                     GFGTMGATGAGTGEAQGCQDGIDLYFSTFAKSMASIGAFISGEKEVIWYLRYNLRSQI
                     YAKSLPMPIVIGNIKRLELLRNNPELRERLWDNVRKLQEGYRSRGFNLGNTDSPVTPV
                     FLEVSIPETINLVVDLRENHGIFCSAVTYPVIPKGMLMLRIIPTASHNDDDIQKTLDA
                     FEAIREKIRTGAYLKEDLVKV"
     gene            4997..5185
                     /locus_tag="PLM90_10895"
     CDS             4997..5185
                     /locus_tag="PLM90_10895"
                     /inference="COORDINATES: protein motif:HMM:NF019076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89927.1"
                     /translation="MELHQKLSRLKAIVSLAEEDLQKFTDKGNKTAGTRLRKSLLEIK
                     EIAGDMRKEVLESKKVKV"
     gene            complement(5325..6245)
                     /gene="dapA"
                     /locus_tag="PLM90_10900"
     CDS             complement(5325..6245)
                     /gene="dapA"
                     /locus_tag="PLM90_10900"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMZ89928.1"
                     /translation="MASKYTAATTHRFRGTGVALVTPFLPEGEIDFAALRTLIDHVIE
                     GGVEYVVSMGTTGESATLTEEERFSIIDATIEHVNGRIPVVGGFGGNDTNAIIRSIEK
                     FRAHRSGKSYIDAILIASPYYNKPSQEGIYQHYLAIDKHVTVPIILYNVPGRTSSNME
                     AATTLRIARDAEHVIAIKEASGNFNQCMYIIKDAPEGFLVISGDDGITLPFISMGMQG
                     VISVVANAYPRPFSDMVRLSLDNKFEGANLIHYKLLAFTDLIFKEGSPAGVKAALKHL
                     NICDEKVRLPLWKISEGLEKKIVDEMNSIG"
     gene            complement(6276..6800)
                     /locus_tag="PLM90_10905"
     CDS             complement(6276..6800)
                     /locus_tag="PLM90_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89929.1"
                     /translation="MPSIKNILFGRFIRSKPYTDTMERSAIPYARIKTMAFLVDGSDP
                     IALRELLNYLHRYTEEGKKITILGYVKKLPPFEEEIIWITAKDKNWIGIPKMFRIKHF
                     IEKEYDVLINTAMKSNRPLEYVSTYSKAKLRIGKYDQQKTFAYDFMMQTENEDVFSYL
                     AQVEHYLRMVKTEP"
     gene            complement(6804..7778)
                     /locus_tag="PLM90_10910"
     CDS             complement(6804..7778)
                     /locus_tag="PLM90_10910"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HMZ89930.1"
                     /translation="MIFLDFEKPIADLYEELEKLKAVGEKQKIDISKPEAELNDKIAQ
                     LRKQIYENLTGWQKVQLSRHPDRPYSLFYIEHMCEDFVELHGDRNVRDDKAIIGGMAT
                     LEGQTVMFIGHQKGINTKMRQYRNFGMANPEGYRKAMRLMKLAEKFNIPIVTLIDTPG
                     AYPGLEAEERGQAEAIARNLYEMTTLRVPVICVVIGEGASGGALGIGIGDRVFMLENT
                     WYSVISPESCSSILWRSWDYKEVAAEALKLTAADNYRNKIIDGIVKEPLGGAHTDPEE
                     SARLLKKHLKKHLKELIPMDPDERIRLRIEKFDDMGVFDEVTPKGANA"
     gene            7910..8341
                     /locus_tag="PLM90_10915"
     CDS             7910..8341
                     /locus_tag="PLM90_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89931.1"
                     /translation="MRIFSIIFASTLVLAGCRQDSKVTHNEGIEHHQFHDEGGLQLDN
                     GKKWQANAETTAGIQIMQERIANFSGNDVDAAALGTALLGDYQTIFDKCTMTGNAHVM
                     LHKYLLPLKPHLDELEACKSSCAEHVVHVKEYLDNYSTYFE"
     gene            8341..9216
                     /gene="prmC"
                     /locus_tag="PLM90_10920"
     CDS             8341..9216
                     /gene="prmC"
                     /locus_tag="PLM90_10920"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMZ89932.1"
                     /translation="MSDKRNQIRYADAVGALKAVLIQHYDVNEASAIARIAGEETFGR
                     DAARSNSLLEMEQLRSWDIITAKLSSGMPLQYALGRAWFMDMTLEVNPSVLIPRPETE
                     ELVELAINQLKAEKLGNGCRLLDIGTGSGCIAIAMKRGLQALEVHAIDISDAAIEIAT
                     RNAKRMHADVHYANANILEPVTLSGLGMFDVIISNPPYIAPAEATGMHKNVVDHEPHP
                     ALFTGDDDPLEFYRAIAAFGRKALHKGGYIFCEISALRGEETKNIFTSAGYNEVQLIT
                     DMQGLPRIVTARHAG"
BASE COUNT         2551 a         2132 c         2002 g         2664 t
ORIGIN      
        1 acccactact cactaccgaa tcgtgaatcg tgagtcgtga atcgtgagtc gtgagtcgtg
       61 agtcgtgagt cgtgaatgtg gtgagtactt gtattagtcc actactcaat acttatacct
      121 gatacttgat acctgatact tgatacccgc ctgtggcgag gcaagcttga tacctgatac
      181 ccgcccgtaa caaggcaagc ttgatgctta tttccccgta agcaactcct gatactgatc
      241 ggcattaacc gggtctagtc ttgtgagata gctcacggct ttggcttttt ctgttggcgt
      301 agccttgctg tataacccca gcaattcatc acttttcgca ctgaagaata attgcatgat
      361 catactattt ggcgaagtgc gattaatatt atcaagtgtt tgcagagcct ttgtaataac
      421 agaaacaggc ttcacctgat cattatataa attatctaaa ccttgacggt gatattgata
      481 aaatgcatca cgcatcacag caaatttcgg atccatgata ttgttgatat accaatacct
      541 gttgcgcgtg ccatcataac ttttccaacc cttcgcatct acattgctcg cctgatttac
      601 gatagtttgt gccttcagaa aatatttatc gccacccttt ggtgcaaatg aatcataatc
      661 aagtccaatg atgacatatg cataataagc cagcattgcc gtaagattac ttgtaaattg
      721 attttcatta aattccaatg cttgatattc ggcatactga aactcaaaat ctttatcctg
      781 aaaattcaac aaagtactat tgtaatcgct cccataaaca ggtcggcgag aaacaatact
      841 ggcttgtgcg cggaatttat cgctcgataa ttcttcagtc accgttatca aaatctgaca
      901 aacaatacgt tcctctggtt taaatgcgtc atctgtccac ttgcgcgtat tcataaactc
      961 aaaaacggag ctttccaggg ttttaaaaat cttcggatcc gtttgtgtta ctttttccgc
     1021 attcacggta acggcacagt ttaattcctg tgcttcgagt cgcgacacga aacctgttat
     1081 caagaggagg attaatatct tacgcatgta atcggcgaat aatatgttgt acaatatcct
     1141 gcgcaacggc agctttactc ttcaattcaa aattaatcac ctcgccgctc ttatccaaca
     1201 gggtgatctt attggtatca tgtccaaagc ctgcaccgcg gtctgctgta gtattcaata
     1261 ctaccatatc gaggtttttc tgtgtaagtt tcgatttggc atattctagc ccattgctcg
     1321 tttctaacgc aaatccaacc acaaaacgcg tgcccttctt agcactgagg gttcccagga
     1381 tgtcggttgt gcgcctcaaa ttgagctgca tcgagccatc ctgactatca ccctttttta
     1441 ttttttgtgc cgatggggcc tccggtgtaa aatcagctac tgctgcagcc gctatgaaga
     1501 tatcgcaggc aggaaaggct tcttcgcaag ccccgagcat ttctgccgct gaggtaactg
     1561 ctatactatg aatatcggca tcatcgcatg taacttgcat tgggccgtgc accaaggtca
     1621 cttcagcacc caatcgcaga catgtttcag ctaaagcaat acccattttt ccggttgaat
     1681 gatttccaat gaaacgaaca ggatctatag cttcatacgt gggacctgcg gttatcagca
     1741 cttttttacc tgaaagtgga ccctttacct gctgaaaatg attttccatc acttcgagca
     1801 attcttcagg ttcagccata cgcccggtac cttctaaccc gcttgctaat tcgccatgaa
     1861 cggggttaat gatgtgatgt ccgaaacctt ttagtttggc gatattgtct accgtagcct
     1921 gatacctcca catatccagg tccattgccg gagccaggta aattggacat ctggacgata
     1981 gggtagtggc aagaagaaag ttatctgtaa tacctcctgc cagtttagca atcgtattgg
     2041 cagttacagg ggcaatcagg atgacatcag gccagagccc ggtgctcaca tgattttgcc
     2101 attgccctgt ttgtttatca aaaaagtcga tggcaacggg gtgtttactt aaagtagcaa
     2161 aggtcagggg gcctacaaat gcggctgcat cgggtgtcat catcacacga acctcggcac
     2221 cggcttttgt gagcaggcgg acaaggtagg cgcttttata tgcagcgatc ccgccacata
     2281 ccccaagtag gaccttcttg ccccgtagcg acatgattag ttggttttag tttcctcagt
     2341 ctcgcgttcg tggaagaaca acttagcgtt gaggaattca tccaatgcca taatggtaga
     2401 tttcggcagc ttttcgtagt atttgctgat ctcgatctgt tctctgtttt cgtggatctc
     2461 ctcaaggtta tcctgatgtg aagcaaattc ctcaagcttg ctgtgcaatt caccgcgaac
     2521 catacctgtg atctgatcgg cacgacgtcc tacaatatag atagaacggt agatgttacc
     2581 gcttgtgtta gcaatgtttt tgagactttg cacttcaatc tgggtagata attgcgtatt
     2641 aattttcttc agagagctca tcgcatttga ttttatatgt ggtaatttct gttattgctt
     2701 tttctttaag gtcagtcgat ttttcaatca aatctttggc gtcagcgact ttcgggctat
     2761 ttggataacg ctgaatgaaa tcctgaccac tattgattgc atcatcgtaa cgttcctctt
     2821 ttttgcagat cacactttga ccggcatact gaaagtagct atcggcaatc ttatagctga
     2881 tttcctcggc atcacgggta tccgggaatt cgaggagcac atttttaaaa gtgatggccg
     2941 cagactcata atgctcggta ttataataaa gttcggcagc gttgataagc ttggtctcca
     3001 gcttgccacg aagctcatcc atgtacttat tcgcctcagg cacgagcgca cttttcggga
     3061 atacgtctac aaaatactga aatgcctcaa tcgcactata agtatactgc tgatctaaat
     3121 tatacctcgg cgaagccata tacatcgatt tggcactcat aaacaacatc tcctcagcct
     3181 caccgcttaa tgggaagata ttgaaaacct ggcggaaaag gcttgatgaa ctgaagtaat
     3241 cagcctgtcc gtagtagcaa tacgcagtgt aataacgcac ctctttttcc tccggggtgc
     3301 caattttcac cgacagcatc tgttccaaaa gcggcagggc acgggcataa tcgcctttgc
     3361 catagtattc cttagctttg gcatacttag cgtcaaaatc atcacttttc aacagcttat
     3421 catattgaca gccggtgaaa actaatatag aaatgcttat aattgtgagt gcaagagccc
     3481 tagtccacgc cataagaggt gcaaagttaa tcattttcgc ctaaacgatg gaaggcttaa
     3541 tttattgaaa aggaggcgtt aaatttttta tccccatatt aacgcaatat ctttgcaccc
     3601 gcaaaaaaga aaacttacac taaaggatat ggatatcttt gacaagttaa gaaaggaccg
     3661 cggtccactg ggcaaatgga gtgatgtagc acacgggtat atgatgttcc ctaaactgga
     3721 aggggaaatc ggccctcgca tgatgttccg cgggaaagag cggatcacat ggagtctgaa
     3781 caattacctc ggactggcaa accacccgga agtgcgtaag atcgatgctg aagcctcggc
     3841 tcagtatggt ctcgcttatc caatgggaag ccgcatgatg agcggtaact ccgatcatca
     3901 cgagcacctc gaaaaagaac tgagcagctt tgttcagaaa gaagatacct tcctgctcaa
     3961 ctacggctat caaggtatca tgagcgtgat cgacgccatc tgcagtcgcc acgacgtgat
     4021 cgtttacgat gccgaaagcc atgcatgtat tatcgacggt gtgcgtctcc atcagggaaa
     4081 acgctttgtg ttccgtcaca acgatatcga aaactgcgaa ttgcaactca aacgcgctgc
     4141 cgaaatggta gaaggcactc cgggtggtat cctcgtgatt accgaaggtg tgttcggaat
     4201 gggtggcgat caaggaaagt tgaaggaaat tgttgaattg aaaaagaaat tcaatttccg
     4261 cttacttgtt gacgacgctc acggcttcgg aaccatgggt gctacaggcg caggaacagg
     4321 tgaagcgcag ggatgtcaag atggtatcga cctctacttc tctaccttcg caaaatcaat
     4381 ggccagcatc ggtgccttca tcagcggcga aaaagaggta atctggtatc tccgctataa
     4441 cctgcgttct cagatatatg ctaagtcatt gccaatgcct atcgttatcg gtaatatcaa
     4501 acgccttgaa ttattgcgta acaaccccga attgcgtgaa cgcctgtggg ataatgtgcg
     4561 caaattacaa gaaggatacc gcagtcgcgg cttcaatctc ggcaataccg atagccctgt
     4621 aacgccggta ttccttgaag tttctattcc tgaaacaatc aacctggttg tcgatttacg
     4681 tgagaatcat ggtatctttt gttctgctgt aacctatccg gttatcccga aaggaatgct
     4741 catgttgcgt atcattccaa cagccagtca taacgacgat gatattcaaa aaacactgga
     4801 tgcttttgaa gccatccgcg aaaagattcg caccggggca tatttgaaag aagacctggt
     4861 taaagtctga ccaaaacgaa attaatctta cagccgggta gaaaattctc ccggcttttt
     4921 tataatttgc acctccttac accttcaacg ggaacgtgaa ccccggcgca ggtaaaacta
     4981 ctaacacact ttgattatgg aactgcatca gaaactcagc cgcctaaaag caattgtatc
     5041 cctcgccgaa gaagacctcc aaaaattcac cgacaaagga aacaaaactg caggcacgag
     5101 actgcgcaaa tcactgctcg aaataaaaga aattgccggt gatatgcgta aggaagttct
     5161 tgaatctaaa aaagtgaaag tatagcatct ggtatcaagc ttgcctcgcc acgggcgggt
     5221 atctggtatc aagtctcaat ccgctttgcg cctttgtttc tttgcgactt agcgcgaaaa
     5281 aaaaaatcct aatgtagaat tggaatccct aaggtcaata cctttcatcc gatactattc
     5341 atttcatcca caatcttttt ctctaatccc tcagagattt tccatagcgg cagacgcacc
     5401 ttttcatcac aaatattaag atgtttcaat gcagctttta cgcctgcagg tgatccttct
     5461 ttgaatatta aatcggtgaa ggcaagaagt ttatagtgaa taagattagc accctcgaat
     5521 ttattatcaa gcgacaatcg aaccatatcg ctgaatggtc tcggataggc atttgccaca
     5581 acagaaataa ctccttgcat gcccatgctg atgaatggca aggtaattcc atcatcacca
     5641 ctgatcacca aaaatccttc aggtgcatcc ttaatgatat acatgcattg attaaaattt
     5701 ccactggcct ccttaatcgc aatcacatgt tccgcatctc gagcaatgcg cagggtagtt
     5761 gcagcctcca tatttgatga ggtgcgaccc ggaacattat ataaaataat cggaacggta
     5821 acatgtttat ctattgcaag ataatgctga taaattcctt cctgcgatgg tttattatag
     5881 tatggtgaag caattaatat cgcatcgata tatgattttc cgctgcgatg tgcgcgaaat
     5941 ttttctattg atcggatgat ggcatttgta tcattaccac caaatccacc tacaacagga
     6001 atacgtccat ttacatgttc aatcgttgca tcaataatgg aaaagcgttc ctcttccgtt
     6061 aaggtggcgc tttcgccggt agtgcccatg cttacaacat attctacgcc gccttcaatt
     6121 acgtgatcta taagtgtgcg caatgctgcg aaatcaattt ctccttcggg taaaaatggt
     6181 gtcaccaatg ccactcctgt tccgcgaaag cgatgtgttg ttgctgcggt atatttgctt
     6241 gccatacttg aaataaataa ttgtaatgaa aataattacg gttctgtttt taccatgcgc
     6301 agataatgtt caacttgtgc gaggtaagaa aatacatctt cattttctgt ttgcatcatg
     6361 aaatcatatg caaatgtttt ttgctgatcg tatttaccga tgcgcaattt tgctttgctg
     6421 tatgttgaaa catattccag cgggcgatta gatttcatcg cggtattgat caatacatca
     6481 tattcttttt caataaaatg tttgatgcga aacatctttg gtataccgat ccagttttta
     6541 tcttttgccg ttatccagat gatttcttct tcaaatggag gcaatttttt tacatatccg
     6601 aggatggtga ttttttttcc ttcttcggta tatctgtgca gataattcaa taattcacgc
     6661 aaagcaatag ggtcactgcc gtccaccaaa aatgccatcg tttttatgcg tgcataagga
     6721 attgcactgc gctccatcgt atcggtgtac ggttttgagc gtatgaagcg accaaataat
     6781 atattcttga tcgagggcat gcgttatgcg ttggcaccct tcggtgttac ctcatcgaaa
     6841 acacccatat cgtcgaattt ctcgatgcgc aagcgaatgc gttcatcagg atccattgga
     6901 atgagttctt tcagatgttt ctttaaatgt tttttgagca aacgcgcact ttcttcagga
     6961 tcggtatgcg caccacccaa tggttctttt acaataccat cgatgatttt attgcgataa
     7021 ttatctgctg cagtaagttt caatgcttcg gcagcaactt ctttataatc ccaactgcgc
     7081 cataaaatgc tcgaacatga ttcaggagaa attacagaat accatgtatt ctccaacata
     7141 aatacgcgat cgccaatacc aattcccaat gcaccaccgg aagcaccttc gccaatcacc
     7201 acacaaatca caggcacgcg caaagttgtc atctcatata aatttctcgc aatagcttct
     7261 gcttgtccgc gctcttcagc ttccagtccc ggataggccc ccggtgtatc gattagcgta
     7321 acaatcggaa tgttgaattt ctctgccagc ttcatcaaac gcatcgcctt gcggtaacct
     7381 tcaggattcg ccataccaaa attgcgatac tgacgcatct tggtgttgat gcccttttga
     7441 tggccgatga acatcaccgt ctgaccctct aatgtagcca taccgccgat gatagccttg
     7501 tcgtcacgca catttctatc gccatgcaat tcaacaaaat cctcacacat atgctcgatg
     7561 taaaacaagc tataaggcct gtcggggtga cggctcaact gcactttctg ccagccggtg
     7621 agattttcgt atatctgttt gcgcaattgc gcgatcttgt catttaactc agcctccggt
     7681 ttagagatgt cgatcttctg cttttcacct actgccttca gcttctccaa ctcctcatac
     7741 aggtcggcta tcggtttttc aaagtcaagg aatatcataa gagcaaaggt aggaaatcgg
     7801 aaggaaactt gaatggcggt gactgttgtg gtatatacac cgctacaatt tggcttaaga
     7861 ttattgaggg tctcgtttgg cgaatgattt ggtcttaact ttacacatta tgcgtatatt
     7921 ttctataatt ttcgcttcca cccttgtttt ggccgggtgc aggcaggatt ctaaagtcac
     7981 ccacaacgag ggaatagagc accatcaatt tcatgatgag ggaggcttgc aacttgacaa
     8041 cgggaagaaa tggcaggcta atgcagaaac cactgccggg attcaaatca tgcaggagcg
     8101 catcgcaaat tttagtggca acgatgtgga tgctgcagca ctaggtacag cattattagg
     8161 cgactatcag accattttcg acaaatgcac catgaccggc aatgcacatg tcatgttaca
     8221 caagtatctg ttgcccttaa agcctcacct tgacgaatta gaggcctgta aatcttcctg
     8281 cgctgaacat gttgtgcacg tgaaggaata tctcgataat tattccactt atttcgaata
     8341 atgtcggata aaaggaatca aatccggtat gcagatgctg ttggggcgct caaagccgta
     8401 ctaatacaac attacgatgt aaatgaggcg tctgcaattg cgcgaattgc aggcgaggaa
     8461 acctttggga gggatgctgc gcgaagtaat tcgctcttgg aaatggaaca gttgagaagc
     8521 tgggatatta tcaccgcgaa attgagttcc ggtatgcctc tgcaatatgc gctaggcaga
     8581 gcctggttca tggatatgac gctggaagtg aatccatcag tgctgattcc acgcccggag
     8641 accgaagagc tagtagaact tgccataaac cagttgaaag ccgagaaatt aggtaacggt
     8701 tgtcgcctcc tggatatcgg tacaggaagc gggtgcattg ccattgccat gaaaagaggt
     8761 ttacaggcat tggaagtgca tgcgatcgac atctctgatg cagctataga aattgcaacc
     8821 cgtaatgcca agcgtatgca tgcagatgtg cattatgcga acgcaaacat ccttgaaccg
     8881 gttacactca gtggtttagg tatgtttgat gtgatcatca gtaatccgcc atatattgca
     8941 ccggctgaag ctaccggcat gcataagaat gtagttgacc acgaaccgca tccggccttg
     9001 tttacagggg atgatgatcc acttgagttt tatcgcgcca tcgccgcatt tggaaggaag
     9061 gcgttgcata aaggagggta tattttttgc gagatatccg ccttgcgtgg agaggaaact
     9121 aaaaacattt tcacatcagc cggatacaac gaagtgcagc ttatcaccga catgcagggc
     9181 ttaccgcgca tagttactgc gcgacatgca ggttagtttt tttcgggact atttttcgaa
     9241 ttcatggaaa aggttatata tttgcatctc tttttgagct acacagctac ggagcggtag
     9301 ttcagttggt tagaatacgt gcctgtcacg cacggggtcg cgggttcga
//
LOCUS       DAOQOU010000132         9280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_3981_length_9280_cov_5.604986, whole genome shotgun sequence.
ACCESSION   DAOQOU010000132 DAOQOU010000000
VERSION     DAOQOU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3981_length_9280_cov_5.604986"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /gene="mscL"
                     /locus_tag="PLM90_10925"
     CDS             complement(<1..294)
                     /gene="mscL"
                     /locus_tag="PLM90_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HMZ89933.1"
                     /translation="MSMLKEFKAFAMKGNLIDISVGFVMGAAFTAVSSAFIQGIVMPF
                     ISLIVQKDFSTWKLRLKAAEKAADGTVIKPEIAVLYGDFISAVIYFLIVAFVMF"
     gene            complement(391..867)
                     /locus_tag="PLM90_10930"
     CDS             complement(391..867)
                     /locus_tag="PLM90_10930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ89934.1"
                     /translation="MEQLFWKIKSFHQLNISELYGILRLRQEVFAVEQNCVYLDVDGK
                     DQFCDHLFGVDDAGRVIACCRIVQPGTSYPEVSIGRVATHPEHRGAGYGKQVMDKAIS
                     FIEDRYGKVPVRIEAQYYLEKFYIDYGFEAVGDVYLLDGIEHREMVRNAVHAKARV"
     gene            complement(1048..2256)
                     /locus_tag="PLM90_10935"
     CDS             complement(1048..2256)
                     /locus_tag="PLM90_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ89935.1"
                     /translation="MKNVFTIFKKEIKDILRDKRTLTVMLIIPLFVYPAIFVGISKYV
                     AGQTEKAGQETLRIAIIDNGNANHFSKFVAGMPNTSIKQIADTTSAQTMINNDSIDVA
                     YYFPAGYDSAIAKLEPTTFEYFYTSTNNEFEVAIIENLNNAYKQQLIKEKLEDNHLSG
                     TILEPAKPVSRNLASSREQIGSMIGGILPYFFIIFCMIGCMYPAIDLAAGEKERGSLE
                     TLLVSSASRLEIYLGKLITVTLSGFISAMASIAGIIMSGKLIGADASQDGGIAQIADL
                     LNGIMEPGSIAMMLLILLPLNIFFAAVTLMLSIYARSFKEAQSMITPLMIFIVFPTIF
                     GMLPGVTLDYGTALVPILNVSLGAKEIISGTVEMGPLLLTYLALFVYAGIALMVSVRF
                     FNNEKNILRG"
     gene            complement(2277..3059)
                     /locus_tag="PLM90_10940"
     CDS             complement(2277..3059)
                     /locus_tag="PLM90_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ89936.1"
                     /translation="MITVDKVSKSFSLSRQQRKELGADHSGKTVNAVNEVSFVCQPGR
                     IFTLLGPNGAGKTTTLRMIATILKPTSGNIIVNNYDTVTQGEQVRGSLGFLTGSTGLY
                     DRLTPNETIDYFGRLNGMDEKTLQDRKKYLFDLLGINEFADRRVGKFSTGMKQKVSIA
                     RTMIHDPQVVVFDEPTSGLDVIAAKSIIELIRQCKKDNKTVIFSTHIMSEVNMLADDL
                     AIIYKGDLIYNGLFTDFKAGMRETTLEDEFIRIAQEHENVTA"
     gene            complement(3065..3943)
                     /locus_tag="PLM90_10945"
     CDS             complement(3065..3943)
                     /locus_tag="PLM90_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ89937.1"
                     /translation="MKSLIKLTSLLTLLPFALNAQQGAKSTSILYAISGNGLTDTSYL
                     FGTFHLINQEHFYVAPEVETAFDNAAQLAFEIKLDDPNMISTYQRWTTLPEGVTWHNF
                     ASDEEIAYMTSVFRDSMQADFTPYIQLKPFVAYQMLTTESMGENAQSYELHFMQRNMQ
                     DHRPIAGLETLESQLKIFDSVPYAEQVDWMTEISDSSFTSEDRLDEMISYYKNGDIES
                     LYNVTITATPEFHDYANLLVADRNKRWIPIIGDLAKKGSTFVAVGAAHLGGPEGVVKL
                     LQQAGYQVTPVKLTEK"
     gene            complement(3940..5763)
                     /locus_tag="PLM90_10950"
     CDS             complement(3940..5763)
                     /locus_tag="PLM90_10950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMZ89938.1"
                     /translation="MQQEQIPLEDFFKNPEKTGFKVSPGGKRISWLANYEQRLNIYIR
                     DSENNIEKRLTARTDRDIRSYFWLTDDQLAFFQDTGGDENEHIFVVDVHTGDTKDITP
                     FEGVKVDLIDELEDVDDAIIIGMNKRNPEIFDVYRLNTITEAITLIEENPGNITSWIT
                     DHTGALRLATTTDGVNTSLLERTAPGQFRTIITTGFRETLAPLFFDFEQKHVYALSNI
                     GRDKIAIVLIDITDAHEIQCLFEHPDVDAGQLGFSKKRKVITSATYTTWKTEFVFFDS
                     ESEQRYAKVKSLINEDAEIYITDRDKAEEVFIVRTLSDRTLGNYYLYHATNNTITHLA
                     NVSPWLQANKLCSMEPISYESRDGLTIHGYLTLPDSGHAPYPVVIHPHGGPWARDVWQ
                     FNPEVQFLANRGYGVLQMNFRGSTGYGRAFLEASFKEWGKKMQDDITDGVQYLIEKGI
                     ADKDRIAIYGASYGGYATLAGLTFTPDLYTCGVDYVGVSSLFSFMETIPPYWAPYLEM
                     MYEMVGHPEKDRELLAEASPLLHIDNIKAPLFVAQGARDPRVKKSESDQIVQALTEKG
                     VDVLYMVRENEGHGFMNMENKFAFYAEMESFLSRHMQSKKK"
     gene            5908..6567
                     /gene="rpe"
                     /locus_tag="PLM90_10955"
     CDS             5908..6567
                     /gene="rpe"
                     /locus_tag="PLM90_10955"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMZ89939.1"
                     /translation="MNPLVAPSVLSCDFAYIARDMEMINRSAADLVHVDVMDGVYVPN
                     ISFGLPVVAAMKRHSTKPLDVHLMMVNPERYLQDFRDAGADSISVHIEACTHIHIAVQ
                     QIHKMGAKAGVAINPGTAVSALEDIIRDVEIVCFMSVNPGWGGQSFIETSIEKIGRLK
                     ELILKTGSKALIEIDGGVKLDNAQRIFDAGADILVSGSGVFGQPDPVAAIASLKSLTR
                     Q"
     gene            6572..9076
                     /locus_tag="PLM90_10960"
     CDS             6572..9076
                     /locus_tag="PLM90_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ89940.1"
                     /translation="MRSALVHLMFRIPLLMALVFAGNLLHAQGKATVYGNVRDEENNP
                     LPEANITLLQPNTTIQTDKQGNYSFEVPAGDTIVLAFTFVGNEPHYEKVILEEGQRYL
                     LLVKLKSITLGTVVVSDPKETIFRIPVRELEQIPSASDPISAILTALGAVKNNELTSQ
                     YSIRGGNYDENLVYVNDFEVYRPLLVRSGEQEGLPFPNYDLIDNIAFSAGGFEAKYGD
                     KLSSVLDIQYKAPREFESGLTASLLGASYYIGDAISTDRDSLIDSTKGYYLFGARYKT
                     NRYLLSSLNTEGQYEPTFIDGQGLFGWKFNRHSSIEALGNFSLNSYTFLPLSRSTATG
                     VVNDVVQLDVFFEGQEIDKFITGFGGFSYRYQRDSFTVYKFLISGYHSLETETFDILG
                     EYWLGQVETNLGSEEFGNVLYGLGVGAFQNFARNYLSSDVFNISHLGTREYNTHYMQW
                     GVRAQYELISDELKEWELLDSAGYSLPYTGETVQLQEVFNSSTTLNSLRYSGYWQDTW
                     EPDSAHISLTAGVRSSYWDLNNEVTITPRIQFVWQPQWKNKLNQRRNIELKASTGLYY
                     QPPFYRELRDLEGNVNTSVKSQKSLHVVIGSDYKFKAWNRDFRFITEMYYKYMFDLIP
                     YDVENVRIRYYGENMATGYAAGIDLRLHGEIVEDADSWVSLSFMRTMEDIEGDSTVTV
                     LDKDSDIDSLLYSPRGYIPRPTDQLVNFGMFFQDYMPGNKNFKVHLSFLIGTGLPYGP
                     PNNEYFRNSLRIAPYRRVDIGFSALLLSPKRDLIGKPWYNTTFESLWASVEVFNLLGI
                     QNTISYIWVKDVNNLQYAFPNYLTDRRINVKLVAKF"
BASE COUNT         2635 a         1950 c         1918 g         2777 t
ORIGIN      
        1 aaacattaca aatgctacaa tgaggaaata aatgacagca gatatgaaat caccgtataa
       61 tacagcaatt tcaggcttga taactgtgcc gtcagcagct ttctcggcgg ctttaagcct
      121 gagcttccag gttgagaagt ctttttgcac aatcaaactt atgaatggca ttacgattcc
      181 ctgaataaat gctgatgata cagcagtaaa ggcggcaccc attacaaaac cgactgatat
      241 gtcgatgaga ttgcctttca tggcaaatgc cttgaattct tttaacatgc tcatgtgata
      301 aaattttaga ggttaatata aattaatgca cgattggcat gcaaggtgga aaaactttgc
      361 gaaatagaca aatgagacat gtggaaaaaa ctatacgcgc gcctttgcgt gtaccgcatt
      421 ccgcaccatt tcccgatgtt caatgccatc aagcaaataa acatcgccta cagcttcaaa
      481 accgtagtct atgtagaatt tttctaaata atattgtgcc tcaatgcgaa caggcacttt
      541 gccatagcgg tcctcgataa aactgatggc cttatccata acctgtttcc cgtaaccggc
      601 accgcgatgc tcaggatggg tggctactcg ccctatggaa acctccgggt agctcgttcc
      661 gggttgcaca attctgcaac aggcaattac cctgccggcg tcatcaacac caaacaagtg
      721 gtcgcaaaat tgatccttac catccacatc cagatagaca cagttctgct caacagcaaa
      781 tacttcctgc ctcaaacgca aaatgccata caactccgaa atattcagtt gatgaaaact
      841 cttgatcttc caaaataatt gttccatacc cgtcgcttaa cctataaacg caaacctgcc
      901 aaaaaaggtt gtatcccgct tactcatttt tccacatctt gtgcggtaat ttccggtttt
      961 gcacaaaaaa tgtgccgatt ttacatcaaa aaaagacctg atccgttttg aagatcaggt
     1021 ctttgtgtaa aaaatatggg cagtgtccta tccgcgcaaa atattttttt cattgttgaa
     1081 gaacctcaca cttaccatca atgcaatacc tgcataaaca aataatgcaa gatacgtcaa
     1141 taacaatggg cccatttcaa ctgttcccga aataatttct ttagctccca gcgacacatt
     1201 taaaatcggt accaaagcag tcccgtaatc taatgtcacc ccgggtagca taccaaatat
     1261 tgttggaaac acaataaata tcatcaatgg tgtgatcatg ctttgtgcct ctttgaagct
     1321 gcgagcatat atcgataaca ttaatgttac tgctgcaaag aatatgttta gcggtaataa
     1381 aatcaatagc atcatggcaa tagaacctgg ttccataatg ccattcagca agtcagcaat
     1441 ttgcgcaata ccaccgtcct gtgatgcatc ggcaccaatc aattttcccg acatgatgat
     1501 acctgctata cttgccatag cagaaataaa tccactcaaa gtaaccgtaa tcagtttacc
     1561 taaataaatt tccagtcgcg atgcagagct tactaataat gtctccaggg aaccgcgctc
     1621 tttctcgcct gcagcaaggt caatggccgg atacatacag cctatcatgc agaaaatgat
     1681 gaaaaaatat ggcagtattc cgcctatcat gctccctatt tgttcgcgtg aagatgccag
     1741 gttgcgggat accggttttg caggttcaag aatagttcct gataaatgat tatcctccag
     1801 cttttccttg attaattgtt gcttatatgc attgttcaaa ttctcaatta tcgcaacttc
     1861 gaattcatta ttcgttgagg tatagaaata ttcgaaagtg gtaggttcta atttcgcgat
     1921 ggcagaatca tatcccgcag gaaaataata ggcaacgtca atgctgtcgt tgttgatcat
     1981 cgtttgtgcc gaagtggtat ctgcaatctg ctttatgctc gtattcggca taccggcaac
     2041 aaatttgctg aagtgattgg cattgccatt gtcgatgatg gctatgcgaa gtgtttcctg
     2101 tcccgccttt tctgtttgac cggcaacata tttagatatg ccgacaaaaa tggcaggata
     2161 tacaaaaagc ggaatgatca acatcacggt gagtgtgcgt ttatcacgca ggatgtcttt
     2221 gatctctttc ttaaatatgg taaatacgtt tttcatttgt catgtagtta aggtgatcag
     2281 gcagttacat tttcgtgttc ttgtgcaata cggataaatt catcctccag agttgtttca
     2341 cgcatacccg ctttaaaatc agtaaacaag ccattataaa taagatctcc cttgtaaata
     2401 atggcaagat catcggcaag catattgact tcactcatga tatgtgttga aaatatcact
     2461 gttttgttgt cctttttgca ctgacgaatc aactcaatga tgctttttgc cgcaataaca
     2521 tctaaccctg atgtcggctc atcaaataca actacttgcg gatcatgaat catggttctc
     2581 gcaatggaaa ctttctgttt cataccggta gaaaattttc ctacacgacg atcggcaaat
     2641 tcatttattc caagcaaatc gaacaaatat ttttttctgt cctgtaacgt cttttcatcc
     2701 attccattca atcgcccgaa ataatctatg gtttcatttg gggtcaatcg gtcatataaa
     2761 cccgtagatc ctgtaagaaa tccgagactg ccacgcacct gctctccttg ggtaacggtg
     2821 tcgtaattat tcacgataat gttacctgaa gttggcttca ggatggtagc tatcatgcgc
     2881 agagttgttg tcttccccgc gccattcgga cctaaaaggg tgaagatgcg tcccggttga
     2941 caaacaaaag aaacttcatt aacggcattc acagttttgc cactgtggtc cgctcccagc
     3001 tctttgcgtt gctgacgcga taatgagaat gatttagaaa ccttatctac ggttatcata
     3061 tggtttattt ttcagtgagt ttaacaggtg taacttgata tccggcttgt tgaagcaatt
     3121 tcacaactcc ttcaggtccg cctaaatgtg cagcaccaac tgctacaaag gtagagcctt
     3181 ttttcgcaag gtcgccaatg atgggaatcc atcgtttatt ccgatctgct accaacaggt
     3241 tagcataatc atgaaattcc ggcgttgcag taatggtaac attatacaga ctttcaatgt
     3301 cgccgttttt ataataactt atcatctcat ccaacctgtc ttcagaagta aatgatgaat
     3361 cagaaatttc agtcatccaa tctacctgtt cggcatatgg cacgctatcg aatattttga
     3421 gttgcgattc aagtgtttct aaacctgcta ttggtcggtg atcttgcata ttgcgttgca
     3481 tgaaatgtaa ttcatacgac tgtgcatttt cacccattga ttctgtggtg agcatttggt
     3541 atgcaacaaa gggttttagt tgtatataag gtgtaaaatc tgcttgcata ctatcgcgga
     3601 aaacagatgt catatatgca atttcctcat cactcgcgaa attatgccag gtaacacctt
     3661 ccggaagcgt tgtccatcgc tggtaggttg aaatcatatt cggatcgtcg agcttgattt
     3721 caaatgccag ttgtgcggca ttgtcgaatg cagtttccac ttccggtgct acataaaagt
     3781 gctcttgatt gataaggtgg aaggtgccaa acaaatatga agtgtctgtc agtccattac
     3841 cactgatggc atagagtatt gaagtacttt ttgccccttg ctgagcattg agtgcaaacg
     3901 gcaatagggt aagtagcgat gttaatttta tgagtgattt cattttttct ttgattgcat
     3961 atgtctgctc aaaaatgatt ccatctcggc ataaaaggcg aatttatttt ccatattcat
     4021 gaatccatgc ccttcattct cgcgaaccat gtacaataca tctacgcctt tttctgtcaa
     4081 tgcctgtact atctgatcgc tttcagattt ttttacgcgc ggatctctgg ctccctgtgc
     4141 aacaaacaag ggtgctttaa tattgtcgat atgcagcagg ggagaggctt ccgcaagtaa
     4201 ttcacgatcc ttttcaggat gtccaaccat ctcatacatc atttccagat acggtgccca
     4261 atatggcgga atcgtttcca taaatgaaaa taaactgctc acaccaacgt aatctacacc
     4321 acaggtatat aaatcaggtg taaatgtcaa tcctgccaga gtggcatatc caccgtagct
     4381 ggcgccataa atggcaatgc gatctttatc ggcaatacct ttttcgatga gatattgcac
     4441 accatcggta atatcatcct gcatcttttt cccccattcc ttaaacgacg cctctaaaaa
     4501 tgcacgccca taacctgtag agcctctgaa attcatttgt aaaacgccat accctcggtt
     4561 ggctaaaaat tgcacttcag gattaaattg ccaaacatct ctagcccagg gtcctccatg
     4621 cggatgtatc accaccggat agggtgcatg tccggaatcc ggtaatgtca aataaccatg
     4681 aatggtaagc ccatcgcgac tttcatacga aattggctcc atgctacaaa gtttatttgc
     4741 ctgcaaccat ggactaacat ttgcgagatg ggtaatagta ttatttgttg cgtgatagag
     4801 ataatagttt cccagcgtgc gatcacttaa tgtgcgaaca atgaatactt cttcagcttt
     4861 atcacgatcg gtaatataaa tttctgcatc ttcatttatc agcgatttta cttttgcata
     4921 tcgctgttca ctttcactat cgaaaaaaac aaattctgtt ttccaggtag tatacgttgc
     4981 cgatgtaatc acttttcttt ttttcgaaaa acccaactga cctgcatcaa catcaggatg
     5041 ttcgaaaaga cattgaattt catgtgcgtc agtgatatca attaacacaa tagcaatttt
     5101 atctcttccg atattgctca aggcatacac atgtttttgc tcaaaatcga aaaacaatgg
     5161 tgctaatgtt tcgcgaaatc ctgtggtgat gatcgttctg aactgtccgg gagcagtgcg
     5221 ttccagtaaa cttgtgttta cgccatcggt ggtcgttgcc aaacgcaatg cacctgtatg
     5281 gtcagtaatc caggaggtaa tatttcccgg attttcttca atgagggtta tcgcttctgt
     5341 tatagtattt aaacggtata catcaaatat ttcaggattg cgcttattca tgccaataat
     5401 tatagcatca tcaacatctt caagttcatc aatcaggtca acttttaccc cttcgaatgg
     5461 cgtaatatct tttgtatcgc cggtatgtac atcaacaaca aatatgtgtt cgttttcatc
     5521 gcctccggta tcctgaaaaa aggcaagctg atcgtcggta agccagaaat atgaacggat
     5581 atcgcgatct gttcgagctg taaggcgctt ttcgatgttg ttttcgctat cgcggatgta
     5641 aatattcaga cgctgctcat aattcgccaa ccacgaaatc cgtttcccgc cgggcgatac
     5701 tttgaatccg gttttttcgg gatttttaaa gaagtcttcc aatggtatct gttcctgctg
     5761 cataattaaa ggcaaaggta tacgcctgac attgcaaagg tggtgcaaga gcggttgata
     5821 gcactatttt ttcgaccagt gttggtaaaa tgtcgacggg atgcaggctt ctaagcgcat
     5881 gtggtttaag ggtggtatct ttgcaccatg aatccgctag tagcacccag tgttctcagt
     5941 tgcgactttg cttatatcgc cagagatatg gagatgatca accgttccgc agcagatttg
     6001 gtgcatgtgg atgtgatgga tggcgtttat gtgccgaata tcagttttgg gttaccggta
     6061 gtggctgcca tgaaacgcca tagcaccaag ccattggatg tgcacctgat gatggtaaac
     6121 cctgagcgtt atctgcagga ttttcgcgat gcaggtgccg atagtatatc tgtacacatc
     6181 gaagcctgta cccatattca catcgccgta caacagatcc acaaaatggg tgccaaagcc
     6241 ggtgttgcta tcaatccggg tactgctgta agcgctttgg aagatatcat cagagatgtg
     6301 gagatcgtat gctttatgag cgtgaacccg ggatggggtg ggcagtcgtt catagagact
     6361 tccatagaaa aaatcggaag gttgaaggag ctgatcctta aaacaggctc taaggctttg
     6421 atagagattg atggtggtgt aaagcttgat aatgcccagc gcattttcga tgccggagca
     6481 gacatactcg ttagcggcag cggcgttttc ggtcaacctg atccggtggc tgccatcgct
     6541 tcattaaaat cgctgaccag acaataactg aatgagatcg gcgttagtgc acttgatgtt
     6601 tcgcattccc ttgttgatgg cactcgtatt tgccggcaat cttctgcatg cccaaggaaa
     6661 agcaacggtg tatggcaatg tgcgcgatga agaaaataat cctcttccgg aagcaaatat
     6721 taccctccta cagccgaata ctactattca aacagataaa caagggaact acagctttga
     6781 agtccctgca ggcgatacca tagtgttggc attcaccttt gttggcaatg aacctcacta
     6841 tgaaaaggta atacttgaag agggtcaacg ctatctgtta ttagtgaaat taaaatctat
     6901 cacgctcgga acagttgttg tcagcgatcc gaaagaaaca attttcagga ttccggttcg
     6961 tgaacttgaa cagatacctt cagcatccga tcctatcagt gccatcctca ctgcattagg
     7021 tgctgtaaag aataatgagc tcacatcgca gtattccatc cgcggcggta attatgatga
     7081 gaacctggtg tacgtaaatg attttgaagt gtatcgtcca ttacttgttc gttcaggtga
     7141 acaggaaggg ctgccatttc cgaattacga cctgattgat aatatcgctt ttagtgccgg
     7201 tggatttgaa gcaaagtacg gcgataaact cagcagcgtt ctcgatattc aatataaagc
     7261 acctcgcgaa tttgaatccg gtcttactgc aagtttattg ggagcatctt attatatcgg
     7321 tgatgccatc agtactgata gagattcatt gatagattcc actaaaggat attatttatt
     7381 tggcgcacgg tataaaacta atcgatacct gctcagttca ttaaatactg aaggtcaata
     7441 tgaaccaaca tttattgacg gacaaggatt atttggttgg aaatttaatc gacattcaag
     7501 tatagaagca cttggaaatt ttagtttgaa tagttatacc ttccttccct taagccgctc
     7561 aacagcaacc ggtgtggtga atgatgttgt gcaactcgat gtgtttttcg aaggtcagga
     7621 aatagataaa tttatcaccg gatttggggg atttagttat cgctatcaac gagatagttt
     7681 tactgtttac aagtttttga tttcaggata tcacagtctg gaaacggaaa catttgatat
     7741 actgggcgaa tattggctgg gtcaggtgga aacaaatctt ggctctgaag aatttggaaa
     7801 tgtattgtat ggattaggtg taggggcatt tcagaatttc gcacgcaatt atttatccag
     7861 cgatgtattt aatatcagtc atctgggcac gcgcgaatac aacacgcatt atatgcaatg
     7921 gggtgtacgt gcacaatacg aattgattag cgatgagttg aaggaatggg aattgctcga
     7981 tagtgcaggc tattcgcttc cgtataccgg agaaacggtg caattacagg aagtatttaa
     8041 ttcctctaca accttaaaca gtcttcgtta tagtggatat tggcaagata cctgggaacc
     8101 ggattctgca catatatcac tcacggcagg tgtgcgaagc agttattggg atttgaataa
     8161 tgaggtgact atcactcccc gtatccagtt cgtatggcag ccacaatgga aaaacaaact
     8221 aaatcagcga cgtaatattg aattaaaagc ctctaccgga ttatattacc aacctccatt
     8281 ttatcgcgag ctccgcgatc tggaagggaa tgtgaatact tccgtaaaaa gtcagaagag
     8341 cttacacgta gttatcggca gcgattataa attcaaagcc tggaatcgcg atttcaggtt
     8401 tattaccgag atgtattata aatacatgtt cgatttaatt ccttacgatg tagaaaatgt
     8461 gcgcatcaga tattatggtg agaacatggc aacaggctac gcagcaggta tcgatctgcg
     8521 tttgcatggc gaaattgttg aagatgccga cagctgggtg agcttgagtt tcatgcgcac
     8581 aatggaagac attgagggcg acagtaccgt taccgtttta gataaagatt cggatataga
     8641 ttctttgtta tattctcctc gaggctatat tccacgtcca actgatcagc ttgtgaattt
     8701 cggtatgttt ttccaggatt atatgccggg gaataaaaat ttcaaagtgc atctcagctt
     8761 tctcattggc acagggttgc cgtatggtcc gcctaataat gaatacttcc gaaacagcct
     8821 tcgcatcgct ccttatcgca gggttgatat cggattttct gccttgttgc tgagccccaa
     8881 acgcgatttg atcgggaaac catggtacaa tactacattt gagagtttgt gggccagcgt
     8941 tgaagtgttc aaccttcttg gcatacaaaa tactatcagt tatatctggg tgaaggatgt
     9001 aaataatttg caatatgctt tccctaatta ccttaccgac cgtcgtatca atgtgaagtt
     9061 ggttgcgaaa ttttaatttc ttccgttaac ttggtgcaat aattactgtt tatgatgcaa
     9121 cgattaattt gcgtgctctt gatattttat tgcggacatg catttggaca acaagtagat
     9181 atcaatgcac ttcagcctga ttctgtttat gaaaatgtat gcagcaagaa aatatattcc
     9241 gattcattga gcactacttt tgttatttgg attaaacata
//
LOCUS       DAOQOU010000133         9251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4000_length_9251_cov_5.894737, whole genome shotgun sequence.
ACCESSION   DAOQOU010000133 DAOQOU010000000
VERSION     DAOQOU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4000_length_9251_cov_5.894737"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1267
                     /locus_tag="PLM90_10965"
     CDS             194..1267
                     /locus_tag="PLM90_10965"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HMZ89941.1"
                     /translation="MSYNNIVSLLGDKAEYLLNHESKTISRDSLHLPGADFVDRVWIP
                     SDRNPQVLRNMEAMFGHGRLANTGYLSILPVDQGIEHSAGASFAKNPIYFDPENIVKL
                     AIEGGCNAVATTYGALGAISRKYAHRIPFIVKINHNEFLTYPNKFDQVMFTSIKASWE
                     LGAAAVGATIYFGSPESTRQLQEVSEAFQYAHELGMSTILWCYLRNPAFKQADKDYHV
                     SADLTGQANHLGVTIEADIIKQKLPENNGGYLALNSKESPYGKNDKRIYEELTSEHPI
                     DLCRYQVANCFMGRAGLINSGGASSGAADLTEAVTTAVINKRAGGMGLISGRKAFQRP
                     MKEGVEILNAIQDVYLTKEVTIA"
     gene            1341..2222
                     /gene="accD"
                     /locus_tag="PLM90_10970"
     CDS             1341..2222
                     /gene="accD"
                     /locus_tag="PLM90_10970"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HMZ89942.1"
                     /translation="MAEQKPDKSSWFKRVRKGIETSTEEKLDTPEGVWWQCPECKKTF
                     TAIEYETNLWCCPNCAYHGRIGSKQYFSILFDEGKHSLICEEVRAKDALNFKDLKPYS
                     DRLKDAWKKSEYTDAITVGTGPIDGHDAVIACMNFEFIGGSMGSVVGERISRAIDYCV
                     AHNQPLLIISKSGGARMMESAYSLMQMAKTSAKLTLLEKHRLPYISICTDPTTGGVTA
                     SYAMLGDVNIAEPKALIGFAGPRVVKETIKKDLPKGFQSAEFVLETGFLDLILERKLL
                     KSNIASLLKHFRNEASA"
     gene            2490..2762
                     /gene="rpsO"
                     /locus_tag="PLM90_10975"
     CDS             2490..2762
                     /gene="rpsO"
                     /locus_tag="PLM90_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HMZ89943.1"
                     /translation="MAVLTAEKKKEIFKDFGGNETNTGSVEAQIALFTNRINHLTDHL
                     KANKKDFATKRSLLMLVGKRRRFLNYLMKKDIKAYRALIEKLNIRR"
     gene            2971..5115
                     /gene="pnp"
                     /locus_tag="PLM90_10980"
     CDS             2971..5115
                     /gene="pnp"
                     /locus_tag="PLM90_10980"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HMZ89944.1"
                     /translation="MKPNPITIEVPLSEGRVVTIETGKLAKQADGSVVVRCGDTMLLA
                     TVVSAKEAKDGVDFMPLTVDYRENYAAAGRFPGGFLKREARPSEYEILISRLIDRAMR
                     PMFPDDYHADTQVLVTLISLDKEILPDALAGLSAAAAIAVSDIPFNGPISEVRVGRID
                     GKFVINPTRTELERSDIDMIVAGSIKDVVMVEGEMKEVKEEDMLEAIRVAHQVIQEQC
                     KKLNELTTLVGKETKRAYSHEVNDQDLKDRMFRELYDKVSAVAHMPSAKEVRGDAFKL
                     IKEEFKATFSEEELAEKSGMIDRYYKDVEKHAMREMILTDRQRLDGRRMDEVRPIWAE
                     VDYLPSAHGSAVFTRGETQSLTTVTLGAKMDEQLIDGVVHEGTSKFLLHYNFPPFSTG
                     EAKPMRGLGRREVGHGNLAMRAIKWSLPEENAYTIRVVSDILESNGSSSMATVCAGSL
                     ALMDAGVQVKKPVAGIAMGLVSEANMVRYGILSDILGDEDHLGDMDFKVAGTVDGITA
                     TQMDIKVDGLSFGVVKEALEQARAGRLHILNEMSKAIQGPRPDLKPHAPRITTIQIPR
                     DYIGAVIGPGGKIIQEMQRESGAEISIEEIGDFGHVVISSPNLESIEKALNRIRAIVA
                     IPEVGEVYEATVKSIMPYGAFVEFLPGKEGLLHISEVDWKRLESLEGILNKGDKLQVK
                     LLEVDQKTGKFKLSRKVLLERPPRPQANNEQH"
     gene            5288..6154
                     /locus_tag="PLM90_10985"
     CDS             5288..6154
                     /locus_tag="PLM90_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HMZ89945.1"
                     /translation="MRQLKITASITSRENDSLEKYLAEIGKIDMITPEEEAVLAKRIR
                     EGDTVALDTLTKANLRFVVSVAKQYQGHGLTLSDLINEGNVGLIKAAKKFDETKGFKF
                     ISYAVWWIRQSIMLGIVEHSRLIRLPLNKAGNVSKVNKLTSQFEQEFERDPTPEEIAE
                     TLHLKDDDVRDIIASNIRHISMDAPLGNDGDDGSMADILADTGEDTPDSGLVSESLRE
                     EIASALKVLSEREAEVVAAYYGISGYPSMTVEEIAKKFNLSRERVRQIKERAIRRLRK
                     ATMSSALKNYLG"
     gene            6343..7593
                     /locus_tag="PLM90_10990"
     CDS             6343..7593
                     /locus_tag="PLM90_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ89946.1"
                     /translation="MGADHYKFYTTIDAMEPMFPEDRSGELEYLATVLMMRASALSES
                     LHPLTRKAIADLLRPMNTYYSNVIEGHDTHPLDIEKALAANFLKNKRQAALQLEAKAH
                     INLHKLIAERFDLKSENPYKKEFICFLHKEFYRHLPGEFLHAERIDGTSVMVIPGQIR
                     DSEVSVGRHVGPYSKSLSAFLDRFEEYYDPTSNNNKSKIKRIISIAAAHHRLAWIHPF
                     VDGNGRVVRLFTDACFMSEDLHSSGLWSMSRGLARHEAAYKFALSAADAGNMNDYDGR
                     GNLSNRELVNFCNFFLSTAIDQVEFMSRMLDIDHMIACIHRFVDLMVAHDKLKKEAAG
                     LLETVFLKGKISKSEAMRITGRSDKTTKLIADSLLEMGLLKLENETKFSDYLVNYPIG
                     FSTVIISGLYPRDKEIDILSRIIS"
     gene            complement(7642..8961)
                     /locus_tag="PLM90_10995"
     CDS             complement(7642..8961)
                     /locus_tag="PLM90_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ89947.1"
                     /translation="MKFFILGIVALWLLVTGGCRLQPDMGTDGPQIFFDEVPNLIADP
                     AHTKVLIVGVLIYDDPALGNFDTYHRKDEELASAFAAYGIPDSNIVILQDEEATRNAM
                     YKALSKIAKNSDENTNFIFYFAGHGFNGWDDDASSIYFANSDIKYLHPDKTGFNLNFF
                     TEEFLPAFEGNSIWLMGDCCKSGGLNDVAANFGATGKQSVALCSCYYTDWSTGNWTFT
                     QKIIDALNGDGMIDKEHDGTITLDDLIAGLSEGLQYVDRQRADCTMYNITGGAFVSDV
                     TIPYSEFTDPQVALGDYVFVYKKHSWYPIEITGKTGTTYTGRRYNFSDYETTTFTVET
                     MKIPYFVHYPIGAEILYDAYTDKPGVIIGADGDFMQMQSTEGGQILWRPYEVIITGTE
                     IPAAILNDDAVWTAGQVLDSLDGNYYVRYNDKNFQWDQWVTPDRVVF"
BASE COUNT         2703 a         1985 c         2088 g         2475 t
ORIGIN      
        1 actggcaaca cgtctgattt tggttcagaa gagtctaggt tcgagcccta gtcggccaac
       61 tgaaaggtct gcataaagca ggcctttttt gttttatcac ccacgtttat aaacccgaaa
      121 acctgcaata ttcttttttt aggcgaaacc cgtaactttg cccctcattc acttagaaat
      181 catacattga cctatgtcgt ataataatat tgttagtctg ctcggcgata aggccgaata
      241 cctcctcaac cacgaatcta aaaccatcag tcgcgacagc ctccacctcc cgggtgccga
      301 ttttgttgat cgcgtatgga taccttcaga ccgcaatcca caggtgctga ggaatatgga
      361 agcaatgttt ggtcacggcc gacttgccaa taccggatac ttaagcattt tgcctgttga
      421 tcagggtatt gaacatagcg caggggcttc atttgccaaa aatcccattt atttcgatcc
      481 cgaaaatatt gtgaaactgg ctatcgaagg cggttgtaac gctgtggcta ctacctatgg
      541 tgccctcggt gccatcagcc gtaaatatgc acatcgcatc ccctttatcg taaaaatcaa
      601 ccacaacgaa ttccttactt atcccaataa atttgatcag gtgatgttca cctctattaa
      661 agcttcttgg gagcttggcg ctgctgctgt aggagcaacc atatacttcg gttctcctga
      721 atctacacgc caattgcagg aagtaagcga ggctttccaa tatgcgcatg aactcggtat
      781 gtctaccatc ttgtggtgct acttgcgtaa ccctgcattc aaacaagccg ataaagatta
      841 tcacgtaagt gccgatctta ccggtcaggc taaccacctc ggtgttacta ttgaggctga
      901 tatcatcaaa caaaaattgc ctgaaaataa tggcggttac ctcgccttaa acagcaaaga
      961 gtctccttac ggaaagaacg ataaacgtat ctacgaagaa ctcaccagcg agcatcctat
     1021 cgacctttgt cgctatcagg tggcaaactg ctttatgggt cgtgcaggtc ttatcaactc
     1081 aggtggtgct tcttcaggtg ctgctgacct taccgaagct gtaaccactg cggtaatcaa
     1141 caaacgcgca ggcggaatgg gactcatcag cggtcgcaag gctttccaac gcccaatgaa
     1201 agaaggcgtt gagattctta atgcgattca ggatgtttac ctgaccaagg aagttaccat
     1261 cgcataacgt atattcgcaa acgcatcacc catgcaaccc gggaaatccg gaatattttt
     1321 tcatgtaaac aagggcgatc atggcagaac agaaacccga taagtcgagc tggttcaaga
     1381 gggtaaggaa agggatagag acttctacgg aagagaaatt agatactccg gaaggggtgt
     1441 ggtggcagtg cccggaatgt aagaaaacat ttaccgccat cgaatatgaa accaacctct
     1501 ggtgctgtcc taactgcgct taccacggac gtatcggttc aaaacaatat ttcagcatcc
     1561 tgttcgatga aggcaaacac agcctcattt gcgaagaggt gcgagctaaa gatgcactga
     1621 attttaagga cctgaaaccg tatagcgaca ggcttaaaga cgcctggaaa aaatctgagt
     1681 ataccgatgc gattactgtt ggcacgggac caatcgacgg gcatgatgct gttatcgctt
     1741 gcatgaattt tgaattcatc ggcggaagca tgggatccgt ggtaggtgag cgcatttcca
     1801 gggctataga ctattgcgtt gcccacaatc aaccactcct catcatatcc aaatcgggtg
     1861 gggcgcgtat gatggaaagc gcttattcgc tgatgcagat ggcaaaaacc tcggctaaac
     1921 tgaccttact cgaaaaacac cgacttcctt atatctccat ttgcaccgac ccgactaccg
     1981 gtggtgttac cgccagctat gccatgcttg gtgatgtaaa tatcgctgaa cctaaagccc
     2041 tgatagggtt tgcgggtcct cgagtggtga aggaaacgat taaaaaagac ctcccgaaag
     2101 gctttcagtc tgccgagttt gtgctggaaa ccggattcct ggatcttatt ttggaacgca
     2161 aattgctgaa aagtaatatc gccagcctcc tcaagcactt ccgcaacgaa gctagcgcct
     2221 gatacagcgt gtacacccga taattgtcag aagttgctga ttatcaaata gttaccggaa
     2281 tgggttcacc gaagcaagga gcagcggatt aaactattaa gtgcccgccg gaaaaagtgt
     2341 aaaagcctga ggtaggctga aggtgccttt tctcccctcc caaaaaaggc tgttttcaaa
     2401 ccggaatcaa tgtttagaag ggttaaaaaa tttgtttgta tggtagatag tgcgtatctt
     2461 tgtgcctcaa attttcaaaa agtatagcga tggctgtttt aaccgcagaa aagaaaaaag
     2521 agatatttaa agattttggt ggcaacgaaa ccaataccgg ttctgttgaa gctcaaatcg
     2581 cattgtttac aaatcgtatc aatcacctca ctgatcacct gaaagcaaat aaaaaagatt
     2641 tcgctacaaa gcgcagtctg ctcatgttgg ttggtaagcg tcgtcgtttc ctcaactacc
     2701 tgatgaaaaa agacatcaag gcttatcgcg cgttgatcga aaaattgaat atacgtcgat
     2761 aatcgtctta tgaaaatatg tatgggctat gcggatattt cattattccc gtagcccatt
     2821 tttcttttgt agtaaccctt aaacaacaat ttatttttaa cctggcagga gtttgatcaa
     2881 cccattttcc ccgttgacaa caaaacccca taaagatctt tcaaactccc gccgcaaaat
     2941 caccctttaa ggagggaatt aatttaatca atgaaaccaa atccaatcac gattgaagta
     3001 ccgctgagcg aaggccgggt agtgacgatc gaaacaggaa aactcgccaa acaggccgat
     3061 ggctctgtag tggtgcgctg cggtgatact atgctcctgg ctaccgttgt atcggcaaaa
     3121 gaagcgaaag atggcgtaga ctttatgccg ttgactgtcg actatcgcga aaattatgct
     3181 gctgccggtc gtttcccggg aggcttcctc aaacgcgagg ctcgtccttc agagtatgaa
     3241 atcctcatca gccgcttgat agatcgcgct atgcgtccga tgttcccgga tgattatcat
     3301 gccgatacgc aagtattggt tacactcatt tcactcgaca aagaaatttt gcctgatgca
     3361 ctcgcaggtt tgtctgccgc agctgcaatc gcagtttctg atattccgtt caatggcccg
     3421 atttctgaag tgcgcgttgg acgtatcgat ggaaaattcg ttatcaaccc tacacgtaca
     3481 gaacttgaac gctccgatat cgatatgatc gttgcgggaa gtatcaaaga cgtggtgatg
     3541 gtggaaggcg agatgaagga agtgaaggaa gaagatatgc tcgaagcaat tcgcgttgca
     3601 catcaggtga ttcaggaaca gtgtaaaaaa ttgaatgaac tcactacact tgtcggcaaa
     3661 gaaacaaaac gcgcatacag ccacgaagta aatgatcagg atctgaaaga tcgtatgttc
     3721 cgtgaattgt atgataaagt atctgcagtt gcacacatgc cttcagcaaa agaagtgcgc
     3781 ggtgatgcat tcaaactcat taaagaagaa ttcaaagcta ctttcagcga agaagaactc
     3841 gcagaaaaaa gtggcatgat agatcgctat tataaagacg ttgaaaaaca tgccatgcgc
     3901 gaaatgatcc tcaccgatcg tcagcgcctc gatggtcgcc gtatggatga agtgcgtcca
     3961 atttgggctg aagtggatta tctgccatca gcacatggtt ccgcagtatt tacacgcggt
     4021 gaaacgcagt cattgaccac tgttaccctc ggtgcaaaaa tggacgagca gttgattgat
     4081 ggtgttgtac atgaaggcac atcaaaattc cttttgcatt ataattttcc tccgttctct
     4141 accggcgaag caaaaccaat gcgtggttta ggtcgtcgtg aagtgggaca tggaaatctc
     4201 gcaatgcgcg ctatcaaatg gtcgctgcct gaagaaaatg cttataccat ccgtgtagtt
     4261 tccgatattt tggaatctaa cggttcatca tcaatggcaa ctgtttgcgc cggttcattg
     4321 gcgttgatgg atgccggtgt gcaggtgaaa aaacctgttg ccggtattgc catgggtctt
     4381 gtatcagaag caaacatggt gcgctacgga atcctgagcg atatccttgg cgatgaagat
     4441 cacctgggtg atatggactt taaagtggct ggtacggttg atggtattac tgccacacaa
     4501 atggatatca aagttgacgg attgagcttt ggtgttgtga aagaagccct cgagcaggcg
     4561 cgtgccggaa ggttgcacat tctcaacgaa atgagtaaag ccattcaagg tcctcgcccc
     4621 gatcttaaac cgcatgcacc acgtatcact acgattcaaa ttccgcgcga ttatatcgga
     4681 gcggttatcg gacccggtgg taagattatt caggaaatgc aacgcgagag cggagcagaa
     4741 atcagcatcg aagaaatcgg cgattttgga catgtggtta tttcttcacc taatctcgag
     4801 tcgatagaaa aagccctcaa ccgcatcaga gcgattgtag ccatacctga ggttggagaa
     4861 gtatacgagg ctactgtgaa atcgatcatg ccttacggtg catttgtaga attcctgccc
     4921 ggtaaagaag gcttgttaca catatctgag gttgactgga agcgcttaga aagtcttgaa
     4981 ggcattttga ataaaggcga taaactacaa gtgaaattgc tggaagtcga tcagaaaacc
     5041 ggcaagttca aactgtcgcg caaggtgcta ttggaacgcc ctccgcggcc acaagctaat
     5101 aacgagcagc attgatctga tcaagctgtt taccataagg catccggaac cacacggatg
     5161 ccttttttca tttctattgg cgctgtgaac tttttgtgca tcaaatatca ttttatggta
     5221 aagagcaacg tttacccata acttttgtaa cttgtgaacg taaacagtaa ttaaacaatc
     5281 ctaagccatg cgtcagctga aaattactgc cagcataacc agtcgcgaaa acgactcact
     5341 ggaaaaatat cttgccgaga ttggaaagat agatatgatt acgcctgaag aagaagcagt
     5401 gctggcaaaa cgcattcgcg aaggagatac tgttgcactt gatacgctca ccaaagcaaa
     5461 ccttcgattt gttgtttccg ttgcaaagca atatcaaggt cacggactta cactcagcga
     5521 tctgatcaac gaaggaaatg ttggtttaat taaagcggca aaaaaattcg acgagacgaa
     5581 aggatttaaa ttcatctctt atgccgtgtg gtggattcgt caatctatca tgttgggtat
     5641 tgttgaacat tcgcgattaa tacgtttgcc attaaataaa gcaggaaatg tgtcgaaggt
     5701 gaataaactc acctcgcaat ttgaacaaga atttgaacgc gatccaacac ctgaagaaat
     5761 tgctgaaaca ttgcatctaa aagatgacga tgtgcgcgat atcattgctt ctaatattcg
     5821 acatatttct atggatgctc cacttggcaa tgatggcgat gatggtagca tggcagatat
     5881 tcttgccgac accggagagg atacgcccga cagtggattg gtaagtgaat cgctgcgcga
     5941 agaaattgct tctgcattaa aagtgctttc tgagcgtgaa gctgaagtag ttgccgcata
     6001 ttatggtatc agcggttatc cttctatgac cgttgaggag atagctaaaa aattcaacct
     6061 cagccgcgaa cgcgtgcgcc agatcaaaga acgcgctatc cgccgcctgc gcaaagcaac
     6121 catgagcagt gcactgaaga attatttggg gtgatttgct gtcagatcga acccggaata
     6181 gaatccggaa atttattcat tttaaatacg gaaaaatttt gcatttgagg gcataaatat
     6241 gcctgaagtg ccttatttaa ttggatttta tgtgattaat ggactcggaa ttgaatacgg
     6301 aaaaaattac tatttgaata cggaaatttg tatttttgca gggtgggagc agaccattat
     6361 aagttttata ctacaattga tgccatggag cctatgtttc cggaagacag atccggtgaa
     6421 ctggagtacc ttgccacagt cctgatgatg agggcctcag cactgtctga atcgctccac
     6481 ccattgacaa gaaaggcgat agctgatttg ttaaggccta tgaacaccta ttattccaat
     6541 gtcatagaag gtcatgatac gcaccccctg gatattgaaa aggcactagc tgccaatttc
     6601 ctgaaaaaca agcggcaagc agcattgcaa ttagaagcaa aagcgcatat taacctgcat
     6661 aagttgattg ctgaaaggtt tgacctgaaa tctgagaatc catataaaaa ggaatttatc
     6721 tgctttctac acaaggagtt ttaccgtcat ttgcccgggg agtttttaca cgctgaaaga
     6781 attgatggca catctgtaat ggtaataccc ggtcaaataa gagacagtga agttagcgtt
     6841 ggaaggcatg tgggtcctta ttcgaaatca ctttctgcat ttcttgacag atttgaggaa
     6901 tattatgacc cgacatctaa caataacaaa tcgaaaataa agcgcattat ttctattgcc
     6961 gctgcacatc acagacttgc atggatacat ccctttgtgg atggcaatgg tcgtgttgtc
     7021 cgattgttta cggatgcatg ctttatgtct gaagatttac attcgtccgg tttatggtct
     7081 atgtcgcgcg gattggctcg gcatgaagca gcatataaat ttgctctttc agcagcagat
     7141 gcaggcaata tgaatgacta cgatggtcgc ggtaatttaa gcaatcgtga gttagttaat
     7201 ttctgcaatt ttttcctgtc aacagcaatc gaccaagttg aattcatgtc gcgaatgctt
     7261 gacattgatc atatgattgc atgcattcat cgcttcgtag atttaatggt tgcacatgat
     7321 aaactgaaaa aggaagctgc cggattatta gaaacagtgt ttctaaaagg taagatttca
     7381 aaatcggaag ccatgcgcat aacgggcaga tccgataaaa caacaaaatt aatagccgat
     7441 agcttattgg aaatgggatt actcaaactc gaaaatgaaa ctaaattttc cgattatctg
     7501 gtaaattatc ctattgggtt ttcgacggta atcatttcag gtctctatcc gagggataaa
     7561 gaaatagata tcttaagcag gattatttct taatcggatt gccggcggta tacatatcaa
     7621 aaaccgcaag gcatattgat atcaaaacac cactctgtca ggagtcaccc attgatccca
     7681 ctgaaagttt ttatcattgt agcgaacata ataattacca tcaagtgaat caagcacctg
     7741 gcctgctgtc cacacggcat catcgtttaa tatcgctgcc ggtatttctg ttccggtgat
     7801 gattacttcg tatggtcgcc acaatatttg cccgccttca gtactttgca tttgcatgaa
     7861 atcgccgtca gcgccaatga ttacgcccgg tttatcggta taggcatcat ataatatttc
     7921 agcaccgata gggtagtgca caaaataggg gattttcatt gtttccactg tgaaggtcgt
     7981 tgtttcgtaa tcggagaaat tatatcgtcg gccggtatat gttgtgccgg tttttccggt
     8041 gatttcaatc ggataccaac tgtgtttttt atacacaaaa acataatcgc ccaatgctac
     8101 ttgcggatcg gtgaattctg aataaggaat agttacatcg ctgacaaaag cacctccggt
     8161 aatattatac atggtgcagt cagcccgctg tctgtcgaca tactgcaatc cttctgacaa
     8221 tcctgcaatc aaatcatcaa gcgtgatggt gccgtcgtgt tctttgtcta tcatcccatc
     8281 gccgtttaat gcatcgatta ttttttgggt gaaggtccag ttgccggtac tccaatcggt
     8341 gtagtagcag ctgcataaag caacggattg ttttcctgtt gcgccgaaat ttgcggctac
     8401 atcatttaag ccaccgcttt tgcaacaatc gcccatgagc caaatgctat tgccttcaaa
     8461 tgcaggtaga aattcttccg taaagaaatt taaattaaat cctgttttat cgggatgtaa
     8521 atatttgata tcactgtttg caaaatagat ggaagaggca tcatcgtccc aaccattaaa
     8581 tccgtgcccg gcgaaataaa agatgaagtt ggtattctca tccgagtttt tggcaatttt
     8641 cgaaagtgct ttatacatgg cattgcgtgt tgcctcttca tcctgcaaaa tcacgatatt
     8701 ggaatccgga atgccataag cagcaaatgc actcgccagt tcttcatctt ttctatggta
     8761 agtatcaaaa tttccgagcg ccggatcatc ataaataagc acgccaacaa tcaatacttt
     8821 ggtatgtgca gggtcagcta taagattagg aacctcatca aaaaaaattt gcgggccatc
     8881 tgtccccata tccggctgca atcggcaacc accggtaact aaaagccaca aagccacgat
     8941 tccaaggatg aagaatttca tggatttcat ttagtgaaga taggtaattg cgttgattcg
     9001 aaaaggttgt ttgtgatttg aagattcgaa ggtttgaaga attgaaggaa gaagtgggcg
     9061 tgcccacatc gcattggtct agatactaga tacttgattc tagatactaa aaaaggttta
     9121 gcagataggt ttcgcgcaaa gacgcaaagt agcaaagccg caaagaaatt cccactcacc
     9181 acccgcctat cggcgagtca agctcaccag tcacccactc actatatttt aatcgcggag
     9241 ttggcggagt t
//
LOCUS       DAOQOU010000134         9194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4045_length_9194_cov_5.597768, whole genome shotgun sequence.
ACCESSION   DAOQOU010000134 DAOQOU010000000
VERSION     DAOQOU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4045_length_9194_cov_5.597768"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..935
                     /locus_tag="PLM90_11000"
     CDS             129..935
                     /locus_tag="PLM90_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ89948.1"
                     /translation="MAQSAAKPAKKTSMDGLGIIVIAVNLVIGILFYKFVCGNPDNFK
                     ANGDPKEGNFLGIVFKGGFIVPVLFALFLTVLVFAFERLLTISKAKGNGSIQSFLQKV
                     KYHLANKDIDAALKECDRQKGSVANVVQAGLHKYAEMKSDTDATTEQKVINISKEIEE
                     ATALEMPALEKHLPILATIASIGTLIALLGTVLGMIRAFAALASEGTPDPSALSAGIS
                     EALVNTAIGIGTSAFAIIFYNIFTGMIDKLTYGIDEMGFSITQTFSAQEK"
     gene            977..1666
                     /locus_tag="PLM90_11005"
     CDS             977..1666
                     /locus_tag="PLM90_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ89949.1"
                     /translation="MPKIKVKRQSTIVDMTAMTDVSFLLLTFFILTAKFRPAQPVIID
                     MPSSESETVVPDNKMVISVDKEGKVYYSIDNPKVRVATLQQLIERYEKTYPSLSKLTD
                     AQIANFANVEMLGSDINQLDQLLNMPPQEINAMHDFSGVPIDSTNNQLRDWIQAGRWA
                     DQIDKTESGTKRDDIRVAIKGDKTSSIKTIQEVIKILTEEPVNIHTFNLITTLEGPVA
                     GGVEASPTPAK"
     gene            1676..2239
                     /locus_tag="PLM90_11010"
     CDS             1676..2239
                     /locus_tag="PLM90_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ89950.1"
                     /translation="MADIQTPDKGSHSKHGKGKVRSKKMSTRVDFTPMVDLAFLLISF
                     FMLTTTLNKPVAMELNMPKKDETVKTDVKESQVLNVILDKEDKIWYYDGRVVYDMQKT
                     DFSADGIRDVIYKKQKEVGQKFGDPKKTIVIIKMTEDANYKNMVDILDEMDITDTKIY
                     AIQDVADLELEAIANGGKLDVIPEGLQ"
     gene            2304..3113
                     /locus_tag="PLM90_11015"
     CDS             2304..3113
                     /locus_tag="PLM90_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMZ89951.1"
                     /translation="MMEDNKKYLNQSMDEIVFDNRNKSYGAYFLRQLTKRNTVKATIV
                     MFVVSGMLTGFTFVDFGFLTKKNDDEAINRVVTLTEPPPLDEAAPPPPPPPPPPPPVR
                     PTVRFVEMVAQRDEDVAEDETITQEEITAEISTQTQEGDEDADIVIETAPVVVDKPVV
                     QEKKVYEFIEQMPEYPGGTEALYNYINSHIQYPRMARDNGVQGKVFLKFIVDENGVVS
                     DVQLLRGIGAGCDEEAMRVVRSLPKWTPGKQNGKPAAVWYKLPIQFTLEDN"
     gene            3180..3425
                     /locus_tag="PLM90_11020"
     CDS             3180..3425
                     /locus_tag="PLM90_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89952.1"
                     /translation="MRNSIFRHPVLLLKLVASVMYVTMGILVLLVPDMLSNMLEGITP
                     LLVNLFAALLIVYGFYRLYRVVTELSEINKPDNDEQA"
     gene            3412..4317
                     /locus_tag="PLM90_11025"
     CDS             3412..4317
                     /locus_tag="PLM90_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMZ89953.1"
                     /translation="MNKLNLLLIITFSLFLGACKDKAWDERTENLNKGTITLYAESSY
                     QTLVEELVKSYENVYPESKIEVRFAPDNAVLDAMFTDKTRMIITGTALSKEQLDRIKS
                     VNEVAAEQYVIGREAIAVIASLSNPDTVFDFDAFLQSRSAGYSGVYEKTGFVFNEENS
                     AMVSQLLGNMTGYNNMFSLGNLDTVVGYVSKNTAAIGFISFAALSDPDDSLTQAILRQ
                     VKVLPVAKADSTGKKIVIPMSQSSIASNDYPFQRYVTVVKGNTPELLGTGFVNFLYRS
                     KASRIMLKAGLVPEKMPERRINIVE"
     gene            4340..5959
                     /locus_tag="PLM90_11030"
     CDS             4340..5959
                     /locus_tag="PLM90_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89954.1"
                     /translation="MKIIKLLLVCLLIQGVAFGQTISDAKHAIESEFYFKAKRMLLNM
                     NKTAPSVESNFYLAEVYLTTGNTDSAKYYYKAASEVTDSKNPLIYVSRGKMLLLNNNA
                     TEAQAQFESAIKASKGKNAEIIHLIGDAYMVAGNVTEAISYYDQAYSTDPNLVINLLA
                     YGDAYLSQDKPGEAMTKYEQAKAVNPNIAVTFLRIARINSKTGKHDAAIAAYKEAIRL
                     DPNLAIAYKELGEESYLAGYYTDVKKYFDEYVKLNAEDKEARLVPAVTCYQLKDYACA
                     IESATQIIAIDSTNFVAWRILYFSHYELGDSIRKTEPDASAKHFADGYQASQKFWSIG
                     EKKLVTLDYQYSAQLAVEAKDTAKAIFYYTLATGSDSTSTYEIAAEYAKYLYTIKKYA
                     ESIVAYNNVIAKYQAGALDYYFLGRAYYQTNDYVNADTIYAQFIALQPNSPDGFLQRA
                     KTKVRLDGIDERGTALPLYLRYIELAEKDIEKNKKNLIDAYLYCFSYYDLMKQTADAC
                     TNFNKAKTLDPNNEYVKQFDAQVNCPTGSSD"
     gene            complement(6037..6891)
                     /locus_tag="PLM90_11035"
     CDS             complement(6037..6891)
                     /locus_tag="PLM90_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89955.1"
                     /translation="MHQIRASITLIPVLAVAVLAGCKKESLDTDTYIALDNAIADGSF
                     QDMMNVVTQQAVANGIDGFSSGTANGKLLEDCPTTTFSEPLGTFPNTMTVDFGVSCTT
                     GYLGVERSGKVIATFTGPYSAEGTTITITTDNYYVNGNKVEGTKTITNEGLNDAGNLE
                     FVVVVVDGVVTLATGETISWNSSRTREWIAGDETLEIEDDVYSVTDGPGVDNAVEGIN
                     RSGTPFTAHIAEPLVRALDCRWFRSGVLEVTPEEMLTRSIDFGSGDCDNVATLSIGEF
                     ETEITLPY"
     gene            7019..7402
                     /gene="rpsF"
                     /locus_tag="PLM90_11040"
     CDS             7019..7402
                     /gene="rpsF"
                     /locus_tag="PLM90_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMZ89956.1"
                     /translation="MNQYETVIIFTPVLSDDDVKRTEESYKDFLRSNGAEIVAEERWG
                     LRQLAYKIKKKSTGIYFVLEWKAPTDVTGKLDIQFNRDENVLRYLTVRLDKYSMDYND
                     RKRRGEIGRKANAKKESGKLQEEGA"
     gene            7456..7671
                     /gene="rpsR"
                     /locus_tag="PLM90_11045"
     CDS             7456..7671
                     /gene="rpsR"
                     /locus_tag="PLM90_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMZ89957.1"
                     /translation="MRKKYCRFKKSGIKYIDFKNPEFLLKFVNEQGKILPRRLTGNSL
                     KYQRKVAQAIKRSRQLALMPYVADLLK"
     gene            7679..8125
                     /gene="rplI"
                     /locus_tag="PLM90_11050"
     CDS             7679..8125
                     /gene="rplI"
                     /locus_tag="PLM90_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMZ89958.1"
                     /translation="MEVILLKDVDNLGDANTLVKVRDGYGRNFLIPRGLAVIANEGNR
                     KMMTERQKAEEARERKLVARIQEVIATLQNGNIRVGAKVGQTDKIFGSITNVQLAEAI
                     KKQTGLVIDRKKITIADEVKTLGTFSANVMLHKDHIVPVSFEVVEE"
     gene            complement(8181..>9194)
                     /locus_tag="PLM90_11055"
     CDS             complement(8181..>9194)
                     /locus_tag="PLM90_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89959.1"
                     /translation="YWVIWHCDFIILLKVRCEAFFESLILDCKFIIMMLHTNSKLLQY
                     TTLASTVLIGEKITAQSVYVDIDPDVLLFEDVWSDEGWKYFDLNEDGILDVGLSYHVG
                     FICGYCPYVIGFDVALLNGAEIATMIAPSLSLWSVYQSSYSSSVGACQIPSHAIADGL
                     AEGEVINMDNQFGVIEELFQTFRCGSYGDFGVQNDLDFQDHNWSTYSKPYIGFQLTVP
                     LGYQYAWLRLYEGDDYNIYVTDFGYQSHVNAGLMIEQPVTDIDESKPQSPYLFSSNGV
                     VHVQDGTGYLMTVTDMQGKEIVEENITSSNFEKRYDLQMGIYLITIVKGDSRFTEMLM
                     LNN"
BASE COUNT         2820 a         1981 c         1952 g         2441 t
ORIGIN      
        1 ggaagctcag ttaacgaaaa aataacaaaa gccgcgtggc ttttcatttt cggtgaattg
       61 agtttttaaa atttcatcta tatttgccaa cacttaaaag ttttacagga aattaaatca
      121 aatcatttat ggctcaatca gcagcaaaac ccgctaaaaa gaccagtatg gatggtctcg
      181 gtattattgt tattgcagta aaccttgtaa ttggtattct tttttacaaa ttcgtttgtg
      241 gaaaccccga caacttcaaa gctaacggcg acccaaaaga aggtaacttc ctcggtatcg
      301 tgttcaaagg aggatttatc gttccggtcc tcttcgcact tttccttaca gttttggttt
      361 tcgctttcga aaggttgcta actatcagca aagcaaaagg taacggctct atccagtctt
      421 tcctgcaaaa agtgaaatat caccttgcca acaaagacat tgatgctgcg ttgaaagaat
      481 gcgatcgtca aaaaggttct gtggcaaacg tggttcaggc cggtttgcat aagtatgctg
      541 aaatgaaatc agatacagat gctaccactg agcaaaaggt gatcaacatc tctaaagaaa
      601 ttgaggaggc tacagcactt gaaatgcctg ctctcgaaaa acaccttcct atcctggcta
      661 ccatcgcttc catcggaacg ctgattgccc ttctcggaac ggtattaggt atgatccgcg
      721 ccttcgctgc ccttgcatct gaaggaactc ctgacccatc ggcactttct gccggtatct
      781 ctgaagccct cgtaaacact gcgatcggta tcggaacatc tgctttcgcc atcattttct
      841 ataacatctt cacaggtatg attgataaac ttacctatgg aattgatgaa atgggcttta
      901 gcattacgca gacattctct gcacaggaga aataatctaa tgcatctttt aaggtgcttt
      961 actaaacgga actacgatgc caaaaattaa agtaaaacga caaagcacga tagtggacat
     1021 gaccgcgatg acggacgttt cattcttgct gctgacgttc ttcatcctta ctgcaaagtt
     1081 ccgaccggca cagcctgtca tcatcgacat gccaagttct gagtctgaaa cagtggtacc
     1141 ggataacaaa atggttatct cggtggataa agaaggaaag gtctactatt caatagacaa
     1201 ccctaaagtg cgcgtggcaa ccctgcaaca gcttatcgaa cgatatgaga aaacatatcc
     1261 ttcactcagt aagctcacag atgctcaaat tgccaatttt gcgaatgtcg aaatgcttgg
     1321 aagcgatatc aatcagttgg atcaactcct caatatgcct cctcaggaaa tcaatgccat
     1381 gcatgatttt tccggtgtgc ctattgactc gaccaacaat cagcttcgcg actggattca
     1441 agccggacgt tgggcagatc agatagataa gactgagtca ggcacaaaac gagatgacat
     1501 tcgtgtcgct atcaagggtg ataaaacttc cagtatcaaa acaatacagg aagtaatcaa
     1561 aatattgacc gaagagccgg ttaatattca cacattcaat ctgatcacaa cattagaagg
     1621 tccggtagcc ggaggagttg aagcctcgcc tacccctgct aaataattta caactatggc
     1681 tgatattcaa accccggata aagggagtca cagtaaacat ggtaaaggca aggtcaggtc
     1741 gaagaaaatg tcgacccgtg tagacttcac ccctatggtg gatctcgcgt tcctgctcat
     1801 ctccttcttc atgctcacta ccacgctgaa caagccggta gccatggagc tgaatatgcc
     1861 gaagaaagat gaaacagtga aaaccgatgt aaaagaatca caagttctaa acgttattct
     1921 ggataaggaa gataagatct ggtattatga tggtcgcgtg gtgtacgata tgcaaaaaac
     1981 cgatttctct gccgatggga tccgcgatgt gatctataaa aagcagaagg aagttggtca
     2041 gaaattcgga gaccctaaaa agactatcgt aatcatcaaa atgactgagg acgcgaatta
     2101 caagaacatg gtagatatcc tcgatgaaat ggatatcact gataccaaga tctacgccat
     2161 tcaggatgtt gccgatctcg aactcgaagc tattgccaat ggcggtaagc tggatgtgat
     2221 tcccgaagga ttgcaataaa aaataatttc ccgatgctta tggaatttta ttcctgccgc
     2281 atcttaacag taaacattcc gctatgatgg aagacaacaa aaaatattta aaccaatcga
     2341 tggatgagat tgtcttcgac aatcgcaaca aatcgtacgg tgcgtatttc ctgcgtcagc
     2401 tcaccaaacg caataccgta aaagctacca ttgtgatgtt tgtcgtaagt ggtatgctta
     2461 ccggttttac gtttgttgac tttggcttcc ttacaaaaaa gaacgatgac gaagccatca
     2521 accgcgtagt aacactcacc gagccacctc cactggatga ggctgcacca ccacctccac
     2581 cacccccacc tccgccacct ccggtacgtc ctactgtaag gttcgtggaa atggttgccc
     2641 agcgtgatga ggatgttgca gaggatgaga caattactca agaagagatc actgctgaaa
     2701 tctctaccca aacacaggaa ggagatgaag atgcagacat cgttatcgaa acagctccgg
     2761 tagttgttga caaacctgta gttcaggaga aaaaggtata tgaattcatc gaacaaatgc
     2821 ctgaatatcc ggggggtacc gaagcattgt ataattacat caactcgcat attcaatatc
     2881 caagaatggc ccgcgacaac ggggttcagg gaaaagtgtt cctgaaattc attgttgacg
     2941 aaaacggtgt tgtttctgat gttcagctcc tgcgcggtat tggcgccggt tgcgatgagg
     3001 aagcaatgcg cgtggttcgc tcgctgccaa aatggactcc gggtaaacaa aatggtaagc
     3061 cggctgccgt ttggtacaag cttcctatcc agtttacatt ggaagacaat taataaataa
     3121 ttcttattcc gataaaacca tctgcggcaa gtccaaggat ggttttattt ttatacccga
     3181 tgcgtaatag tatctttcga cacccggtgc tgctgctcaa actcgtggct tcagttatgt
     3241 acgtaacaat gggaatactg gttctcctcg ttccggatat gctgagcaac atgctggaag
     3301 ggatcactcc cctactggta aacctgtttg ctgcattgtt gatcgtttat ggcttttacc
     3361 gcttgtaccg ggtggtgaca gagttatctg aaatcaataa acctgacaat gatgaacaag
     3421 cttaaccttc tgctgatcat cacattttcc ctatttctcg gggcttgtaa agacaaagcc
     3481 tgggacgaaa ggactgaaaa cctcaataaa ggcactatca ccctatatgc cgaaagcagc
     3541 taccaaaccc tggtggagga actggtgaaa tcgtatgaaa atgtctatcc tgagtcgaaa
     3601 atcgaggtcc gattcgcccc tgacaatgca gttttggatg ctatgttcac cgataaaacg
     3661 cgcatgatca ttaccggaac cgcactttcg aaggagcaat tagaccggat caagagcgta
     3721 aatgaggtag cagcagagca atatgtgata ggtagagaag ccattgcggt gatcgcttct
     3781 ttgtcaaatc ctgacaccgt atttgatttt gatgcatttt tgcagagtcg ttctgccggc
     3841 tattccggtg tatatgagaa aacgggcttt gttttcaatg aggaaaatag cgccatggta
     3901 tcacaattgt tgggtaatat gaccggttac aacaatatgt tttccctagg caatttggac
     3961 actgtagtgg gttatgtatc caaaaacacc gctgccatcg gatttatctc ctttgcagcg
     4021 ctcagcgatc ctgatgactc gttaacacaa gcaatattaa ggcaggtaaa ggtgcttccg
     4081 gtagcaaagg ccgattcaac gggcaaaaag attgtaattc ccatgtcaca atcctctatt
     4141 gcatccaatg attatccttt ccagcgttat gttacagtgg ttaaaggaaa cacacctgaa
     4201 ttattaggca cgggatttgt caacttctta tataggagca aggcaagccg cataatgtta
     4261 aaggccggac ttgttccgga gaaaatgccc gaaaggcgaa ttaatatagt agaataacaa
     4321 tcaataagac cgacctaaaa tgaagatcat taaactgttg ttagtgtgcc tgttgataca
     4381 gggtgtagca tttggacaaa ctatcagtga tgccaaacat gctattgaat ctgaatttta
     4441 cttcaaagca aaacgcatgt tgctgaatat gaataaaaca gcaccgagcg tagaaagcaa
     4501 tttctatctt gcagaagtat atcttactac cggaaatacc gactctgcca agtattatta
     4561 caaagcagcg agtgaggtta cggattctaa aaatccattg atctatgttt cacgtggtaa
     4621 aatgttgttg cttaacaaca atgctaccga agctcaggca cagtttgaat ctgccattaa
     4681 agccagtaaa ggaaaaaatg ccgagatcat acacctgatc ggcgatgcct atatggttgc
     4741 cggtaatgtg actgaagcaa tcagctacta tgatcaggca tattcaactg atcctaattt
     4801 ggtgatcaac ctgcttgcct atggcgatgc ctatctgagt caggataaac ccggcgaagc
     4861 aatgaccaaa tatgaacagg caaaagcagt aaacccaaat attgctgtta cattcttgcg
     4921 catagcccgt attaactcta aaacaggtaa gcacgatgct gccattgctg catataaaga
     4981 agccatccgc cttgatccaa atcttgccat agcatataaa gaacttggtg aagagagcta
     5041 tttggcaggt tactataccg acgtaaaaaa atacttcgac gaatatgtaa aactcaatgc
     5101 cgaagataaa gaagctcgcc tggtgcctgc agttacatgt tatcaattaa aggattacgc
     5161 atgtgctatt gaatctgcaa cacagattat tgctattgac agcaccaatt ttgttgcatg
     5221 gcgtattctt tatttttcac attatgaatt aggtgattct atccgcaaaa cagaacctga
     5281 tgcatcagcg aaacattttg ccgatggata ccaagcttca caaaaattct ggagtatcgg
     5341 tgagaaaaaa ttggtgacac ttgattatca atactctgca caattagctg tcgaagctaa
     5401 agatactgcc aaggctatat tctattatac acttgctacc ggcagtgatt ctacatcaac
     5461 gtatgagata gcagctgaat atgcgaaata tctttatacc attaaaaaat atgccgagtc
     5521 aattgtcgca tacaataatg tgattgctaa atatcaggca ggagcattgg attactattt
     5581 ccttggtcgc gcatattatc agaccaatga ttatgtaaat gcagatacca tctatgcaca
     5641 attcattgcc ctgcaaccaa attcacccga tggattttta cagcgtgcga aaacgaaagt
     5701 tcgtctcgac ggtattgatg aacgcggaac agcattgcca ctttacctca ggtatattga
     5761 attagctgaa aaggatatcg aaaaaaataa gaaaaacctt attgatgcct atttatattg
     5821 tttctcatat tacgatctga tgaaacaaac tgccgatgcc tgcactaatt ttaataaagc
     5881 caaaacattg gatcctaata atgaatatgt aaaacaattt gatgctcagg taaactgccc
     5941 gacaggtagt agcgactgag attagtccga aataataaaa aaggctgtca tgtttatcac
     6001 gacagccttt ttttttatgg tttcaggctt taagaattaa tacggtagcg tgatttcagt
     6061 ttcaaattcg ccaatagaaa gtgtagcaac attgtcgcaa tcaccactgc caaaatcaat
     6121 agaacgggta agcatttctt caggtgttac ttcaagcacg ccggagcgga accatcggca
     6181 atcgagggct cgaaccaatg gctcggcaat atgcgctgta aagggtgtgc cggagcgatt
     6241 aatgccttca acagcattgt caactcccgg gccatcagtt accgaataaa catcatcttc
     6301 tatttccaag gtctcgtccc ctgctatcca ctcgcgggtg cgtgagctgt tccaggaaat
     6361 agtttctccg gtggcaaggg ttaccacgcc atctaccacc acaacaacaa attcgaggtt
     6421 acccgcatcg ttgaggccct cattggtgat ggtttttgtg ccttcaacct tattgccgtt
     6481 tacatagtaa ttgtcagttg tgatggtgat ggtagttcct tcagcggaat acggtcctgt
     6541 gaaggttgca atcactttac cggagcgctc aacgcccagg tatcccgttg tgcagctcac
     6601 cccgaaatca acggtcattg tgttcgggaa ggtgccgagg ggttcgctga aagtggttgt
     6661 agggcaatct tccagtagtt taccattggc agtgccggag ctgaatccgt cgatcccgtt
     6721 ggctaccgct tgctgggtga caacattcat catgtcctga aaagacccgt cggcaatggc
     6781 gttgtcgagg gcaatgtagg tatcagtatc gagactttct tttttacatc cggccagtac
     6841 ggcaaccgcc agtacgggga tgagggtgat ggaagcacgg atttggtgca tatatctaag
     6901 atttggaggt ttagacaaag ttaatggctg agggtttaac gctttggctt ttttggatat
     6961 tatgggggta tttgctatct ttgcagtcca atttctaaaa aaattaataa caacgaaaat
     7021 gaaccagtac gagacagtca tcattttcac tcctgtcctc tcagatgacg acgtgaagag
     7081 gaccgaggaa tcctacaagg atttccttcg ttcgaacgga gctgaaatag ttgccgaaga
     7141 gcgctggggc ctcaggcaac tggcctacaa gattaagaaa aagagcaccg gtatttactt
     7201 tgtgctcgaa tggaaagcac caaccgatgt aaccggaaag cttgacatcc aattcaaccg
     7261 cgatgagaac gtattgcgtt atctgacggt gcgtttggac aagtattcta tggattacaa
     7321 cgaccgcaag cgtcgcggag aaatcggaag gaaggcaaat gcgaaaaaag aatcaggtaa
     7381 acttcaagag gagggagctt aatcatggca acaagtaaga acattcgtta cctcgcagcc
     7441 cctaagttgg gtgccatgcg caagaaatac tgccgtttca agaagagcgg aatcaagtat
     7501 atcgacttca agaaccctga gttcctgttg aaatttgtta atgaacaagg caagatcctg
     7561 ccacgtcgcc tcaccggtaa cagcctgaaa tatcagcgca aagttgctca ggcgatcaaa
     7621 cgcagtcgcc aattggccct catgccttat gttgctgacc ttttaaaata atatagccat
     7681 ggaagtgata ctcttaaaag acgtagacaa cctgggtgat gcgaataccc tggtaaaagt
     7741 gcgcgacggt tacggacgta acttcttgat cccgcgcggc ctggctgtta ttgccaatga
     7801 aggtaaccgc aaaatgatga ctgaacgtca gaaagcggaa gaagcacgtg aacgcaaact
     7861 cgtagcaaga atacaagaag ttattgccac cctccaaaac ggaaatatcc gcgttggtgc
     7921 taaagttggt caaaccgata aaatctttgg ttcaattacc aacgtgcaat tggcagaagc
     7981 tatcaaaaaa caaaccggat tggttattga tcgcaaaaag atcactatcg ccgacgaagt
     8041 gaaaacattg ggaacattca gtgcaaatgt aatgttacac aaagatcata tcgtaccggt
     8101 atcctttgaa gtagttgaag aataaaggtt ggagaatata cagtaaaaga ggctgtcctt
     8161 cgggatggcc tttttttatg ttaattgttg agcatcaaca tttctgtaaa tcgggaatca
     8221 cccttgacaa ttgttatcag ataaattccc atttgtaaat catatcgctt ctcaaagttt
     8281 gaggatgtaa tattctcttc aacaatttcc tttccctgca tatctgtcac tgtcatcaga
     8341 tagccggttc catcctgcac atgcacaact ccgttactgc taaacaaata tggcgattga
     8401 ggttttgatt catcaatgtc tgttaccggt tgttcaatca tgaggcctgc attcacatgt
     8461 gattggtatc caaaatcagt tacataaata ttataatcat cgccttcata caatcgtaac
     8521 catgcatact gatacccaag aggaacagtc agttgaaatc caatataagg cttagaataa
     8581 gtgctccaat tatgatcttg gaaatctaaa tcattctgca ctccgaaatc gccatatgaa
     8641 ccgcatcgaa aagtctgaaa tagctcctca attactccaa attgattatc catatttatc
     8701 acttctcctt cagccaaacc atcggctatt gcatgagatg gaatttgaca tgctcctaca
     8761 gacgaggaat atgatgattg gtatacactc caaagtgata atgacggcgc aatcattgtt
     8821 gcaatttctg ctccgttcaa taatgccaca tcgaaaccaa taacatatgg acaataaccg
     8881 caaataaatc ctacgtggta gctcaagccc acatcaagaa tcccatcttc gttcaaatca
     8941 aaatatttcc atccttcatc actccataca tcttcaaata atagcacatc gggatctata
     9001 tcaacgtata cagattgtgc tgtaattttt tctccaatca gaactgttga agctagcgtt
     9061 gtgtattgga gtaatttgga attggtgtgc aacatcatga tgataaattt acaatccaaa
     9121 attaatgatt caaagaaggc ttcacaacgc accttgagca aaatgataaa atcacaatgc
     9181 caaatgaccc aata
//
LOCUS       DAOQOU010000135         9159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4070_length_9159_cov_5.934315, whole genome shotgun sequence.
ACCESSION   DAOQOU010000135 DAOQOU010000000
VERSION     DAOQOU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_9159_cov_5.934315"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="PLM90_11060"
     CDS             <1..1922
                     /locus_tag="PLM90_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ89960.1"
                     /translation="FLMKKILLTIMASAGLLLADAQLMIDATGVPVLIDFTGFSGSGF
                     QPGGGGGMLNSDEWSVTGFSEGGVDFGATATTGDFARGSTMGLVTSGGVYAVDIAGNQ
                     GLMVQPTADDFTPGSFILKIENNTGVDVTEIAVAYTIYVLNDQNRSNSFNLSVSYDNI
                     TYTPITAVDYTSPDVMDFTPYVVPKATNITGLDFPDGTVMYLSWTGDDVGGAGSRDEF
                     ALDDVSITAIEGAPVSLATFAPAADVVDETAGTASVNISLSDNADCTVHVSMNAASTA
                     DGADATFAAFDHTFDIAGDPLYTFNIPLTDDLLDEPDETLILDLTYIAGACAVGLPST
                     FTLLIMDDDVTPPPTYTVYDIAEIHGEDANGVATSIDAHAEITGVVHGINTWNGGLQF
                     TLIDATDGISVFSYDNDFGYTVTEGDEITIQGVVTQFNGLTEIEPDTLWFIDGGNALE
                     TSSIVTTLSEATESHMTTITNLSYVDVAEWLGDGSSFNVNLTNGVNTYTIRIDDNNEL
                     STMTAPSLGTPYTLSITGIGGQYDTGSPYLDGYQLNPRYAADIVVVPTVGIYAPEQYA
                     IAVYPNPVIDACTITANELINSIRVINALGQVVYSASVNANHVSLQTGDWNAGLYQIQ
                     LDINGNLSNTQVVVE"
     gene            2122..3102
                     /locus_tag="PLM90_11065"
     CDS             2122..3102
                     /locus_tag="PLM90_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HMZ89961.1"
                     /translation="MSKLSGYLRAIYHHEEYHGWFKRRRFFEGWYFKIVDKTQQHAFA
                     FIPGIAMDEQGNRQAFIQVLDGIHVTADYYKFDREFFHPNDRRFKVKIKENSFSMNRI
                     KLQLPEIRGQVHFSEHVKLPKTLLWPGIMGWYSYVPLMECYHQVISMDTELTGKLVVR
                     GQEIDFTGGRGYIEKDWGRSFPSSWIWMQSNHFANPKTSFKLSVAKIPWLNSSFVGFI
                     SVFLLNGKILRFATYTGAKLKEVAINENGTEVCLEDKRYILRVNAVKAAGAELKSPIQ
                     GLMEGKVKESMQAVMHITLFDKHENRLIFNDTGNNTGLEIAGDTHELLQF"
     gene            3083..3655
                     /locus_tag="PLM90_11070"
     CDS             3083..3655
                     /locus_tag="PLM90_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89962.1"
                     /translation="MNFCSFKSLLRFGTTLLLFLPILLCAQDTIGYSEFRFTVIDVQG
                     NELASDKITAQIGDIFLRDTTQVDNYAAARVEYSLRDSCWILSQYAPRGYNYEIEIFR
                     NSDTVNLRLLELRKMTLIYPGYNEIDQTGCQFCLCYDIPIQKGVYMLDVPQRRESWKY
                     LRQVYINVRNLPTEFRDISAMQNWMFRSGK"
     gene            3736..4899
                     /locus_tag="PLM90_11075"
     CDS             3736..4899
                     /locus_tag="PLM90_11075"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HMZ89963.1"
                     /translation="MMTVRGDFNRFYAGFFIAITCLSSSCKNDASTSSDLETKVVADY
                     ADHVFNTYTETLSAAKYLQSAVDGFLAAPSDSTLQEARNGWRIARAYYGKTEAFRFYD
                     GPIDNGEHGVEGLLNAWPLDEAYVDYVEGNSSTGIINDIKTYPEITRDLLVSLNEKGG
                     EENISCGYHAIEFMLWGQDLDTIAAGKRPYTDFIAGSLINDRRRTYLKLVTDLLMEHL
                     QYVTDQWSPKIQDNYRTAFLEADPKESLQKIFTGIGVLAKVELAGERMFTAYDNASQE
                     DEQSCFSDNTYADLQSNLDGIARIFFGNEGRTGLRQLLESKDPTAAQNVETQLKATQQ
                     AFEHMHQPFDRAIAVDAYRAETMQAILAVQKLGNDIANAASALQLTINVTNVQ"
     gene            4896..6335
                     /locus_tag="PLM90_11080"
     CDS             4896..6335
                     /locus_tag="PLM90_11080"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HMZ89964.1"
                     /translation="MMQVIQAHIKTIIAIAAIGVFLFFITIGSCDKENLLAAASLTSE
                     ADEALSGGSATVFDQTVNAFALPVPGLSPEDELLFFVGNSFFNQNWVQAPSSTTARDG
                     LGPYFNAKSCSGCHFKDGRGEPPATIGQLSEGLIVRLSIPGNGAHGEPVPDPMYGGQF
                     QDHAIDGVAYEGQYEIVYVEVPGYYPDGTSYSLRKPQINFLDLQYGAMSSDIMYSARV
                     APQMCGLGLLEAISEKDILAHADPDDRNGDGISGRANYVWNAIENKEQIGRFGWKANQ
                     PTILQQVSGAFNGDIGITSPYFKDQHITDAQSAQRKLVNGGDPEIDNDDLEKVHLYSA
                     TLAVPARRNVDDQDVLSGKQLFIKLGCNACHLDKVVTGNHPKFSVLSNQTIHPYTDLL
                     LHDMGEGLADGRPDFQASGSEWRTPPLWGIGLFKTVNKHTYYLHDGRARSIEEAILWH
                     GGEASTAKASFMQLSKAERDQLLLFLESL"
     gene            6428..8650
                     /locus_tag="PLM90_11085"
     CDS             6428..8650
                     /locus_tag="PLM90_11085"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ89965.1"
                     /translation="MTRIFTFLACICLCLLSTNLQAQTFSHVENTWVPDDGSSITFAI
                     DVSGLPDVIDTVFGLETVCINMDHTWDSDLDIRLVAPDGTSFLLVSGIGGDGDNFNNT
                     CFNESAATPVSFGSAPFSGSFIPMGDMGIANNGQNPNGVWQLLVYDTYAFADQGYMYD
                     WSITFGDDPATSSTFDSSILPIVKINTGGQIIQNEPKVTAAFQIIDHGAGMLNHPTDT
                     DYAFDGKILVELQGFTGPYYPKKNYDFDIVDDAGLEYDTVLLGLPSENDFILKAEYLD
                     LSLMKNQIAYTMSRNMGRYAPRTKYCEVLVDGEYMGVFSLTEKIKRDDNRVDIAKLDS
                     IDISGEELTGGYIIEINENFTPNDWESDYLPINYATCGLPVAYKMVEPRIENIQPEQL
                     DYIVAYVDSFEDALHGPDFLDTAVGYRNYISVKSFIDFMLVNEFSSNYDSYGRSTFLY
                     KEKNGQLFIGPPWDYDRGYLPWTTEGWVWEITHPAWPFPFWWDKFRDDPEFRNEVYCR
                     WTALRADVLSDEAFDTMIDSLAEAIGPDAQMRNFDKWPELGVTDPDYFVEEVRTFLHQ
                     RLAWMDEALEVDAVTGIDASFASEQVGLNLVQFTPSDLDADTYFWNFGDGTTSTEINP
                     LHTYGGIGDYIVSLDVVKYYGCAAMTYSPVQVVVHVDNIENADITVMPNPATHVVNIS
                     GLQSAASIEILDITGKVVSTLFTESEIDISAFPAGQYYLRITNKDVVRVLTFVKVDMQ
                     "
     gene            complement(8709..9137)
                     /locus_tag="PLM90_11090"
     CDS             complement(8709..9137)
                     /locus_tag="PLM90_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HMZ89966.1"
                     /translation="MYPKELVMPMKAELTAVGFEDLSTPELVTNAIKNTEGTYLLVFN
                     SVCGCAAGSARPGVKMSLANEVKPDHIGTVFAGYDMEAVAEARKLTLPYPPSSPSVAL
                     FKNGKLVYFMERHMIEGRNAMMIAAELANAYNKHCVQVEG"
BASE COUNT         2636 a         1864 c         2014 g         2645 t
ORIGIN      
        1 tatttctgat gaagaaaatt ttactgacaa ttatggcatc agccggttta ttactcgccg
       61 atgcacagtt aatgattgat gccacaggcg taccggtgtt aattgatttt acaggctttt
      121 ccggctcagg gtttcaaccc ggtggcggag gcggtatgct taattccgat gaatggagcg
      181 ttaccggttt cagtgaaggt ggtgtcgact tcggcgccac tgcaactaca ggcgatttcg
      241 caagaggaag cacaatggga ctcgttacct caggtggtgt ttatgcagtt gatatcgcag
      301 gtaatcaagg attaatggtg cagccaacag cagatgattt tacccccgga agtttcattt
      361 taaaaattga aaataatacc ggtgtcgatg ttacagaaat tgcagtagct tacactattt
      421 atgtgctcaa cgatcagaat cgtagcaaca gctttaacct gtcggtatcg tacgataata
      481 ttacctatac acctatcacc gcagttgatt atacttctcc ggatgtgatg gattttacac
      541 cgtacgtagt accaaaagca actaatatca ccggtctcga ttttcctgac ggaacagtaa
      601 tgtatttgag ctggaccggc gatgatgttg gtggtgccgg atcacgtgat gaatttgctt
      661 tggacgatgt atccattacc gctatcgaag gtgcgcctgt ttcattggca acttttgctc
      721 ccgctgctga tgtggtagac gaaactgcag gcaccgcatc cgtgaatatt tcactcagcg
      781 ataatgcaga ttgcactgtg catgtcagca tgaacgcagc ttcaacggct gatggtgcag
      841 atgctacatt cgctgccttc gatcatacat tcgatattgc tggtgatccg ctctacacat
      901 tcaatattcc attgaccgac gatttattgg atgaacctga tgaaacgctc atccttgacc
      961 tcacatatat tgcaggtgcc tgcgctgtcg gacttccatc aacatttact ttgttgatta
     1021 tggatgatga cgttacacct ccgccaacat ataccgtata cgatatcgct gaaatccatg
     1081 gcgaagacgc taacggtgtg gcaacttcaa ttgatgcgca tgctgaaatt acaggtgtag
     1141 tacacggcat taatacctgg aatggcggat tgcaatttac attaatagat gcaacagacg
     1201 gcattagtgt atttagctat gataatgatt tcggttatac cgtgactgaa ggcgatgaaa
     1261 ttactattca aggtgttgtc actcaattta atggacttac agaaattgaa cctgatacac
     1321 tgtggtttat tgatggcgga aatgcattgg agacttcatc aattgttact acgctctctg
     1381 aagcaaccga atcgcatatg acaacaatta ctaaccttag ttatgttgat gtagctgaat
     1441 ggttgggaga tggttcatca tttaatgtaa accttaccaa tggtgtaaat acctatacca
     1501 ttcgtattga tgataataat gaattgtcaa ccatgacagc accgtcgctc ggaacgcctt
     1561 atacactttc tatcacaggc attggaggcc aatacgacac aggctctcct tacctcgatg
     1621 gttatcagct taatccacgt tatgcagcag atatagttgt ggtgccaacg gtaggcattt
     1681 atgctcctga acaatatgct atcgctgttt acccgaatcc tgttatagat gcctgcacca
     1741 ttacagctaa tgagttaatc aactcaatcc gcgtgattaa tgcgcttggt caggttgttt
     1801 actcagcatc agtgaatgcc aatcatgttt cactgcaaac cggcgattgg aatgcaggct
     1861 tgtatcaaat tcaactcgat atcaacggaa acttgagcaa tacgcaagtg gtagttgaat
     1921 aatttattgt taaatcatat ttcaaaagag gggtcagact gctgatccct cttttttatt
     1981 ttcagtgtgc agaagcttct cctaaactac ttgtcagcac tgtcattata tcgtaaatat
     2041 ggcgttcctt aatacattct taacttatca taacagcaat actgtattaa ttgagtaagt
     2101 ttgatgtata tatttctgcc aatgtcgaaa ttatccggat acctccgtgc gatttatcat
     2161 catgaagaat atcatggatg gtttaagcga cgtcgctttt ttgagggatg gtattttaaa
     2221 atcgtagaca aaactcaaca acacgcattt gcttttattc ctggcattgc catggacgaa
     2281 cagggcaaca gacaggcttt tattcaggtg ttggatggca ttcatgttac ggctgattat
     2341 tataagttcg accgcgaatt ttttcatccc aacgaccgcc gattcaaagt aaaaatcaaa
     2401 gagaacagct tctctatgaa tcgcatcaaa ttgcaattgc ctgaaatacg cgggcaagtg
     2461 catttcagcg aacatgtcaa acttccgaaa acattattgt ggcccggtat catgggatgg
     2521 tattcttatg tgcctttgat ggaatgttat catcaggtga ttagcatgga tacagaactt
     2581 accgggaaat tggtggtgcg cggacaagaa attgatttta ccggaggaag aggatatatc
     2641 gaaaaagatt ggggacgctc attcccttcc tcatggatct ggatgcaatc aaatcacttt
     2701 gcaaatccta aaacttcatt caaactttct gttgcgaaaa ttccctggtt gaatagttca
     2761 tttgtcggat ttatcagcgt attcctgctc aatggaaaaa tattgcgttt cgcaacatat
     2821 accggtgcga aattgaaaga agttgccatc aacgaaaacg gaacggaagt atgcctcgaa
     2881 gacaaacgct atatccttcg cgtaaatgct gttaaagccg cgggtgccga attgaaaagt
     2941 cctattcagg gactcatgga aggcaaggtg aaagagagta tgcaggcagt tatgcacatc
     3001 acccttttcg acaaacatga aaatcgcctt atttttaatg ataccgggaa taataccgga
     3061 ttagaaattg ccggcgacac acatgaactt ctgcagtttt aagtctttgc tgcggtttgg
     3121 aactaccttg ttattgttcc tgccaatatt gctttgcgca caggatacca tcggttattc
     3181 cgaattccgt tttacagtta tcgacgttca gggaaatgaa ctggctagtg ataagatcac
     3241 tgctcaaatc ggcgatattt ttcttcgcga taccactcaa gtcgataatt atgctgccgc
     3301 cagagtagaa tattctttaa gagattcttg ctggattctg agccaatatg caccacgtgg
     3361 ctataactac gaaattgaga tttttcgcaa tagcgatacc gtcaatctgc gtctcctcga
     3421 gttgcgcaaa atgactctta tctaccccgg atacaatgaa atcgatcaaa caggatgcca
     3481 attttgcctc tgctatgata ttccaattca aaaaggtgtc tacatgctcg atgtcccgca
     3541 acgccgtgaa tcctggaaat acctccgcca ggtctatatc aacgtacgca acctacccac
     3601 cgaattccgc gatatcagtg ccatgcagaa ctggatgttc cgcagcggaa agtaagcaaa
     3661 tacctactta tgggtattga aaaaaagcac tcctgctacc tacatttgca ccttatttag
     3721 actagtttta aattaatgat gacagttcgg ggggatttta accgctttta tgccggtttt
     3781 ttcattgcaa taacttgttt atcaagtagt tgtaaaaatg atgcttctac atcttccgac
     3841 ctcgaaacca aagtggtggc agattatgcc gaccatgtat tcaataccta cacagaaact
     3901 ctcagcgctg caaaatacct gcagtcggcg gttgacggct ttttagcagc accctcagat
     3961 tcaacccttc aggaggcacg caacggctgg agaatagcaa gagcatatta cggcaaaact
     4021 gaagctttcc gcttttacga cggacctata gacaatggag aacatggtgt tgaaggcctc
     4081 ctcaatgctt ggccgttgga tgaagcgtac gttgattatg tagaaggaaa ttcgtcgacc
     4141 ggaataatca atgacatcaa aacctatcct gagatcacac gcgacttgct ggtgtcatta
     4201 aatgaaaagg gtggggaaga gaatatttct tgcggttatc atgctattga atttatgttg
     4261 tggggacaag atttagatac tatcgcagca ggtaaaagac catataccga ttttattgca
     4321 ggtagcctga tcaacgaccg cagacgcacc tatttaaaac ttgtaacaga tttgttgatg
     4381 gaacatctgc aatatgtaac agatcagtgg tcgccgaaaa ttcaggataa ttatagaacc
     4441 gcatttttgg aagcagatcc caaagaatcc ttgcaaaaaa tatttaccgg aattggcgtg
     4501 cttgccaaag tagaacttgc cggtgagcgc atgtttactg catatgataa tgccagtcag
     4561 gaagacgaac aaagttgctt tagtgataat acttatgcag atcttcaaag taacctcgat
     4621 ggtatagcac gcatattttt cggtaatgaa ggtcgcaccg gattgcgtca attattggag
     4681 tcaaaagatc caacggcagc acaaaatgtg gaaacacaac tcaaggcaac acaacaagca
     4741 tttgaacaca tgcatcagcc attcgacaga gctattgcgg ttgatgcata tcgcgctgaa
     4801 acgatgcagg caattctggc agtgcaaaaa cttggaaatg atattgcaaa tgccgcatct
     4861 gcacttcagt taaccatcaa tgttactaac gtgcaatgat gcaggtcatc caggcacata
     4921 ttaaaaccat cattgcaata gctgccattg gtgtattttt atttttcatc acaataggtt
     4981 cctgcgataa agaaaattta ttagcggctg catcattaac ttcggaagca gatgaagcgt
     5041 tatcaggtgg atcggcaaca gtgttcgatc agacagtaaa tgccttcgcg ctgcctgtac
     5101 cgggactttc accggaagat gaattgttat tttttgtcgg aaattctttc ttcaatcaaa
     5161 attgggtgca ggcgccaagc agcacaacag cgcgcgatgg attgggtccg tattttaatg
     5221 caaaatcttg ttcgggatgt cattttaaag atggaagagg cgagcctcct gctaccattg
     5281 gtcagttatc agaaggatta attgtaagat taagtattcc cggaaatggc gcacatggag
     5341 aacctgttcc tgatccgatg tatggcggtc aatttcaaga ccatgctatt gatggtgttg
     5401 cttacgaagg gcaatatgaa attgtttatg tagaagttcc cggatattat cccgatggca
     5461 cttcttattc cttgagaaaa ccacaaatca attttcttga tttgcaatat ggagcaatgt
     5521 catctgacat catgtattcg gcacgtgttg ctccgcaaat gtgtggtctt ggtttattgg
     5581 aagcgatttc ggaaaaagat attcttgctc atgccgatcc tgacgatagg aatggcgatg
     5641 gcatttcagg tcgcgctaat tatgtgtgga atgcaattga aaataaagag caaattggtc
     5701 gcttcggatg gaaagcaaat caaccgacaa tattacagca ggtgagcggc gcatttaatg
     5761 gcgatatcgg aattacttca ccctacttta aagatcaaca tatcaccgat gcgcaatctg
     5821 ctcagcgtaa attggtgaat ggtggcgatc ctgagattga taatgatgat ttggaaaaag
     5881 tacatctcta ttcagcaaca ctcgctgttc ctgcacggag aaatgtcgat gaccaagatg
     5941 tgctcagtgg gaaacaattg ttcattaaat taggttgtaa tgcctgccat ctcgataaag
     6001 ttgttaccgg caatcacccg aaattttctg tgttatcgaa tcaaaccatt cacccttata
     6061 ccgaccttct gttacacgat atgggtgaag gactcgccga tggtcgccct gactttcaag
     6121 catcaggaag cgaatggcgc acaccaccac tgtggggaat cggcttgttt aaaacggtaa
     6181 ataaacatac ttattatctc cacgacggca gagcgcgttc tatcgaagaa gccattcttt
     6241 ggcatggagg tgaagcttca acagctaagg cttcatttat gcaattgagc aaagctgaac
     6301 gagatcagtt gctcctcttc ctcgaatcat tgtaaaacaa tagtcatcct tttttaattg
     6361 caggcaaagg gttggtatag tattaccata atgccggcaa atatgtaatt tcgtgttgcc
     6421 tatttgtatg acacgcatat ttactttctt agcctgcata tgtctatgcc tgctttccac
     6481 gaaccttcaa gcccaaacat tttctcatgt tgaaaatacg tgggtgccgg atgatggatc
     6541 cagcatcact tttgcgattg atgtttccgg acttcccgat gtgatcgata ccgtgttcgg
     6601 acttgaaaca gtttgcatca atatggatca tacctgggat agtgacctcg acattcgttt
     6661 ggtggcgcct gatgggacca gttttttgct tgtaagcgga ataggaggag atggcgataa
     6721 tttcaacaac acttgtttta atgaatctgc agcaacgcct gtgagtttcg gatctgcgcc
     6781 attcagtggg tcttttattc caatgggcga tatgggaatt gcaaacaatg gtcagaatcc
     6841 gaatggtgtg tggcaattgt tggtttacga tacttatgca ttcgctgacc agggatatat
     6901 gtacgattgg tcaataacct tcggagatga tcctgccacg tcttctactt tcgacagttc
     6961 aattctgcca attgtgaaaa taaataccgg cggacaaatt attcagaatg aaccaaaagt
     7021 aactgccgct tttcaaataa ttgatcatgg tgccggtatg cttaatcacc ctacagatac
     7081 cgattacgca ttcgacggaa aaattcttgt ggagttgcaa ggatttacag ggccctacta
     7141 tcctaaaaag aattatgatt tcgatattgt tgatgatgca ggtcttgaat atgataccgt
     7201 attgctcgga ctcccatcag aaaacgattt cattctgaaa gcagaatacc tcgacctgtc
     7261 gctgatgaaa aatcaaattg cttacaccat gtcgagaaat atgggacgat atgcaccaag
     7321 aacgaaatat tgtgaagtgc tggtagatgg agaatatatg ggtgtgtttt cattgacaga
     7381 aaaaataaaa cgcgacgata atcgggttga tattgccaag ttagattcaa ttgacatttc
     7441 aggtgaagaa ctcaccggcg gatacatcat tgaaatcaac gaaaatttta cgccaaatga
     7501 ttgggaaagc gattatctgc caataaatta tgctacatgc ggattaccgg tagcttataa
     7561 aatggtagaa ccgcgcatcg aaaatatcca accggagcaa ctagattata tcgttgcata
     7621 tgtcgacagt tttgaagatg cacttcatgg ccccgatttt ttagatactg ctgtaggtta
     7681 tagaaattat atatctgtaa aatcattcat tgatttcatg ctggtaaatg aattttcttc
     7741 aaactacgac agctacggca gaagtacatt tttatataaa gaaaaaaatg gtcaattatt
     7801 cattggtccg ccttgggatt acgaccgcgg ttatttgccc tggacaacag aaggctgggt
     7861 ttgggagatc acacatcctg catggccttt ccctttttgg tgggataaat ttcgcgatga
     7921 tccggaattc agaaatgaag tatactgccg ctggacagcc ctcagagctg atgtactaag
     7981 cgatgaggca tttgatacaa tgatcgactc tttggcagaa gcaataggtc ctgatgcaca
     8041 aatgcgcaat ttcgataaat ggcctgaatt gggggttaca gatcctgatt attttgttga
     8101 agaggtgcgc acattcctgc atcaacggtt ggcgtggatg gatgaggcac tggaggtgga
     8161 tgcagtaacc ggcatcgatg catcatttgc ttcggaacaa gtgggattga atttagtgca
     8221 atttactcca tcagatctcg atgccgatac ttatttctgg aattttggcg atggcaccac
     8281 atccaccgaa ataaatccac tacatactta tggtggaatt ggcgattata ttgtcagcct
     8341 tgatgtggtc aaatattatg gttgcgctgc aatgacctat tcaccggtgc aggttgtggt
     8401 acacgttgat aatattgaaa atgctgatat cactgtaatg ccaaatcctg ctacacatgt
     8461 tgtcaatatt tcaggattgc agtcagcggc atctattgaa atattggata ttacaggaaa
     8521 ggtagtttcg acattattta cagaaagtga aatcgatatc agtgcttttc ctgcaggcca
     8581 atattattta cgcattacga acaaagatgt tgtacgcgta ctgacatttg tgaaagttga
     8641 catgcaataa aaaatccgtc atcggaatag gtatgcattc caatgacgga tgtataatct
     8701 gaaaaaaatt atccttctac ctgcacacaa tgcttgttat aagcatttgc caattcagca
     8761 gcaatcatca tggcattgcg accttcaatc atatgtcgct ccatgaaata cactagtttg
     8821 ccatttttaa ataaagccac actaggtgag cttggagggt agggaagtgt aagcttgcgt
     8881 gcttcggcaa cagcctccat atcgtagccg gcgaaaacgg tgccgatatg atcgggtttt
     8941 acttcattgg caagcgacat ttttactccc ggacgcgcac tgccggcagc acagccacat
     9001 actgagttaa acaccagcag gtaggtgcct tctgtgtttt taatagcgtt ggttaccaat
     9061 tcaggagtcg acaggtcttc gaagcctaca gccgtaagtt cggctttcat tggcattacg
     9121 agttcttttg gatacatgtg cgaaaatgtt aattatgct
//
LOCUS       DAOQOU010000136         9122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4094_length_9122_cov_5.649057, whole genome shotgun sequence.
ACCESSION   DAOQOU010000136 DAOQOU010000000
VERSION     DAOQOU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4094_length_9122_cov_5.649057"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLM90_11095"
     CDS             complement(<1..880)
                     /locus_tag="PLM90_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217703.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HMZ89967.1"
                     /translation="MEQEKMSGGKCPFPHEMLKPVAGRGTRNQDWWPNQLKLNILRQH
                     SSLSNPMDPDFDYAKAFNSLDFKALKKDIEDVMTTSQAWWPADYGHYGPFFIRMAWHS
                     AGTYRIADGRGGGGSGTQRFAPLNSWPDNANLDKARLLLWPVKKKYGNKISWADLMIL
                     AGNCALESMGFKTFGFGGGREDVWEPEEDIYWGSEGKWLDDKRYSGDRDLENPLAAVQ
                     MGLIYVNPEGPHGNPDPLAAARDIRETFARMAMNDEETVALIAGGHTFGKAHGAGPAS
                     NVGPEPEAASVEEQGLG"
     gene            1126..2526
                     /locus_tag="PLM90_11100"
     CDS             1126..2526
                     /locus_tag="PLM90_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ89968.1"
                     /translation="MRNILFVIACMNAAIAPAQVIFSSKPVFGFAYQQETNDPFPVIK
                     EVTLGSPAFLANLMVGDHLLRINNADLRDISTDALTAILRDAPQMQNVITVQHVDATK
                     TEIKLNKANYITTEYESFRYTIFFDANSSPIYKEGENCLWTTTDCYNGYGYYVFDAYG
                     AFYGEFENGNRKEGLYYHPVFGKPTYYLGEFLNNEYEGLGTLQYLSDDSIVRVYKGNF
                     HNGAPFGQGEIHTADGRLEYTGGFNGWDYNGQGKYIFPDGTVKEGLWTNGQFADDIVM
                     PSTETPDNTGYTAEEIEKADKCRKAGLTEKEIRNFIMELRNPYSSDNSSSAGTTETLS
                     GIDYETSVWNEVMKNYGYGLLEEFDIGFSINEIGTCFTGCSTGQRRIMLLIPHVYGIQ
                     VSDLYVVTSRLSGDISDVNYEHFPFELISSDGDYTVYNCNIVASEIHSGCSYQWQVLS
                     EDGTRRFVRVLVFAIL"
     gene            2659..3219
                     /locus_tag="PLM90_11105"
     CDS             2659..3219
                     /locus_tag="PLM90_11105"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HMZ89969.1"
                     /translation="MQLEVRIKKQFLISGIIGSLVLCGIAFMILIYSTPGMSSPWRYV
                     VQALQVIIILFSIPNAILGIRRLIGSRIGLRISDEGVSDITVANAGRMYLWKEIKAIR
                     LHDANQVKIPFIAIELLDPDIFMHGKIGVARKILQTNIAKYGTPVVIRPTVLDIDPHI
                     LLDALREGLDEFTTSQVHNEQAEVIN"
     gene            3260..4279
                     /locus_tag="PLM90_11110"
     CDS             3260..4279
                     /locus_tag="PLM90_11110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HMZ89970.1"
                     /translation="MIEIGIDSFAAKPSRLGISDAQAINELLERIVYADEVGLDVFGL
                     GEHYRKEFLDSASYMLLAAAASRTKNIKLTSAVTVLSAADPVRVFQNFATLDLISNGR
                     AEIVTGRGSFIESFPLFGYDLHDYDALFTEKLDLLLKIRDNEFITWEGAFRPALQHQP
                     VYPRPMQEKLPIWIGVGGTPASFARAGALGLPLMVAIIGGETRRFKPLVDLYRQAGAK
                     AGFAPEELQVGLHSLGYVANTKEEAIADYYPGYAEMFTKIGIERGFGPVTRGGFDAQN
                     GHYGALLVSDPEEVAHKIIRHSESLGGISRFTFQMDAGIPHEKLMQAIHLIGTKVKPI
                     LMDAK"
     gene            complement(4323..5096)
                     /locus_tag="PLM90_11115"
     CDS             complement(4323..5096)
                     /locus_tag="PLM90_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ89971.1"
                     /translation="MIEIRDISKSFGEQHVLKQVSAVFESGKTNLVIGISGSGKTVLM
                     KIMTGLITPDAGKVFFNDRDYYDLSRSEQKLLRREIGMLFQGSALFDSLTVEENVMLP
                     LNMFSNDTQKERLDRVNFCLDRVNLAGANKKYPGELSGGMKKRVGIARAIVLNPRYLF
                     CDEPNSGLDPRTSIVIDDLIIEITKEYNMTTIINTHDMNSVMEIGEKIVFLYKGVKDW
                     EGNKDEILQAKDENLNSFIFASDFLRDARDALKEKKGLL"
     gene            complement(5093..5845)
                     /locus_tag="PLM90_11120"
     CDS             complement(5093..5845)
                     /locus_tag="PLM90_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ89972.1"
                     /translation="MNIEFNIFQHLGKFLIMIGRMFSRPERWRMYFKEMFRQMVDIGV
                     GSAFIVLLVSIFIGAVTAVQTAYQLYGTPFVPSSYIGLIVRNTIIFELAPTFTALILA
                     GKVGSNLASELGSMRVTEQIDALEIMGVNTESYLISTRVMAAIITIPILVSVAAYSGI
                     MGGYIATAAGSNYSVEDYVRGIRMYFDPFDVQLMFIKSFAFSFIVSSISCYMGYYVNG
                     GSVEIGRASTKAVVYSCIFILLADYVIAALLL"
     gene            complement(5899..6945)
                     /locus_tag="PLM90_11125"
     CDS             complement(5899..6945)
                     /locus_tag="PLM90_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ89973.1"
                     /translation="MAQVDSTHTDTVHRSKGKFFPSPVLGYSPETRLYFGAGLVWYLP
                     PSKKYPNSNASVIKAVGVYTLNKQIESNISGESYFGDNLYKLNYTTNFFKFPDAFYGI
                     GNQNSPDDKESYNYDYFNLQVNGERLVFPKFFAGLKTFIETTKVYDVKEGGIFDTEDV
                     PGEDGGLNTGIGPWLTYDTRDNIYFPLKGLNADFSAVFHSKLTGSNYTYVDYMFEFSQ
                     FNKMMRDDVLGFNVYAQFVPGEAPFNRLAQLGGDRHMRGHYEGRYRDINYIAVQAEYR
                     ITFWKYFGVNIFAGTGEVAPDLNSFSTAGLHYSYGVGGRLFIVPEDKLSLRVDYGFDE
                     EGNGSLYVTFREAF"
     gene            7301..8806
                     /gene="atpD"
                     /locus_tag="PLM90_11130"
     CDS             7301..8806
                     /gene="atpD"
                     /locus_tag="PLM90_11130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMZ89974.1"
                     /translation="MSGKGKIKQIIGAVVDVAFEEGSLPEILNALEVKRENGEALYLE
                     VQQHLGEDSVRTIAMDSTDGLVRGAEVIDLGKNISMPTGDAIRGRLFNVVGTAIDGMR
                     PVDSDVRYPIHRKPPLYEELSTEAEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGKTV
                     LIMELINNIAKGYSGMSVFAGVGERTREGNDLLREMIESNVIRYGEDFKHSMEQGGWD
                     LSKVDYSQLNQSQATLVFGQMNEPPGARARVALSGLTVAEYFRDGDPNEAKGRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLASEMGLMQERITSTKRGSITSVQAVYV
                     PADDLTDPAPATTFSHLDATTVLNRKISELGIYPAVDPLDSTSRILSADIVGEEHYRC
                     AQRVKGILQRYKELQDIIAILGMEELSEEDKLVVSRARRVQRFLSQPFHVAEQFTGLK
                     GVLVPIEETIRGFNMIMDGEVDEYPEAAFNLVGTIDDAIEKGKKLLADAKK"
     gene            8902..>9122
                     /locus_tag="PLM90_11135"
     CDS             8902..>9122
                     /locus_tag="PLM90_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HMZ89975.1"
                     /translation="MQLEILSPEKKIYSGEAVGVQMPGITGSFEVLNNHAPMIAALAN
                     GRMRVNTGKDNIVYNIKGGFVEVVNNTVSV"
BASE COUNT         2677 a         1946 c         1923 g         2576 t
ORIGIN      
        1 aaccaagacc ttgttcttca acactagctg cttcaggttc agggcctaca ttagaggctg
       61 ggccagctcc gtgagcttta ccaaatgtat gtccaccagc aatcaacgcc actgtttctt
      121 catcattcat agccatgcgc gcaaatgttt ctctgatatc gcgtgccgca gcaagagggt
      181 caggattgcc gtgcggacct tcagggttta catagatcaa tcccatttga acagcagcaa
      241 gcggattttc taaatcacga tcgccggaat agcgtttatc gtcaagccat tttccttctg
      301 atccccaata aatatcttct tcaggttccc atacgtcctc acgtccgcca ccgaaaccga
      361 atgttttgaa tcccatggat tccagcgcgc aatttcctgc caatatcatc aaatcagccc
      421 aggaaatttt attgccgtat ttctttttca ccggccacaa taacagtcgc gctttatcca
      481 aattcgcatt gtcgggccag ctgttcaatg gtgcgaatcg ctgcgtgccg gaaccaccac
      541 caccgcgacc atcggcaatt cgataagtgc ctgcgctgtg ccaagccatg cgaatgaaaa
      601 atgggccgta atgaccataa tctgccggcc accatgcttg tgaggtggtc ataacatcct
      661 caatatcctt tttcaatgcc ttaaagtcca gactattaaa agcctttgca tagtcaaaat
      721 caggatccat cgggtttgaa agcgatgaat gctgacgtaa tatgttcaat tttagctgat
      781 tcggccacca gtcttgattg cgggttcctc gacctgccac gggtttcaac atttcatgcg
      841 gaaagggaca ttttcctccg ctcatttttt cttgttccat atgatgtttg attttctgct
      901 atttgcatta tcctgcaaac ctaatgaaca tttcggttct gcggtatgac ttacgtacac
      961 tacaaatttc tgccgaaata aattcctaca tgcaatcaca ttcgcggcaa tcacttttgg
     1021 cagtgtagaa tgcagtttcc ttacctgaaa cttgatattg taactaactt accgttctgg
     1081 accaaaattt atagctagat tggagatcta gttcaaatga aacacatgcg aaacatatta
     1141 ttcgtcattg cctgcatgaa cgctgcaatt gctccggcac aggtaatttt tagcagtaaa
     1201 cctgtttttg ggtttgccta ccaacaagaa acgaacgacc catttccggt aataaaagaa
     1261 gtgacattgg gaagccctgc attcttagca aatttgatgg tcggcgatca tctgctgagg
     1321 ataaacaatg ctgatctccg agatatttca acggatgcac taacagccat tttgcgggac
     1381 gcacctcaaa tgcaaaatgt aattacagtt cagcatgtgg atgcaacaaa aaccgaaatc
     1441 aaactcaata aagcaaatta tatcacaact gaatacgagt catttcgtta tactatcttt
     1501 tttgacgcta acagtagccc tatttataag gaaggtgaga attgtttgtg gacgactaca
     1561 gattgttata atggatatgg atactatgta tttgatgctt atggtgcatt ttatggagag
     1621 tttgaaaatg gaaacaggaa agagggactc tactatcatc cggttttcgg aaagcctacc
     1681 tattatttgg gagaattttt aaacaatgag tacgaaggat tgggaacctt gcaatatctg
     1741 tctgatgata gcatagtacg tgtttataag ggaaattttc acaacggagc accatttgga
     1801 caaggcgaaa ttcacacagc tgatggcaga ttagaatata ccggcggatt taatggatgg
     1861 gattataatg gccaggggaa atatattttt cctgatggca cggtaaaaga aggtttgtgg
     1921 acgaatggac aatttgctga tgatatcgtt atgccgtcga cagaaacacc cgataatacc
     1981 ggatatactg cagaggaaat tgaaaaagcc gataaatgcc gcaaagccgg tctgacggaa
     2041 aaggaaatac gaaattttat catggaatta cgcaatccat attcatcaga caattcatcc
     2101 tctgccggca caacggaaac tttgtcgggc attgattatg agacaagtgt atggaatgaa
     2161 gtaatgaaaa attatggtta tggactctta gaggaatttg atataggatt ttctataaat
     2221 gaaattggca cttgttttac cggttgcagt accgggcaaa gaaggataat gctgcttata
     2281 ccacatgtgt atggaattca ggtatctgat ttatatgtgg tgacatctcg tttatcagga
     2341 gatatcagcg acgtcaatta tgagcatttt ccatttgaat taatcagcag tgatggcgat
     2401 tatacagtat acaattgcaa tatagttgca tccgaaatcc actccggatg ctcataccaa
     2461 tggcaggttc tatctgagga cggcaccagg cgatttgtgc gagtgttggt atttgcaata
     2521 ctatgattac ttaaaggaaa atcattaaat tttaactttc atgtttacat gcataccaat
     2581 tttgaattgc cgattatcat ctgatacagc cggcccaata gaaaacaata tatcatttat
     2641 gtattctacc tttgtgccat gcaattagaa gtgcgcataa aaaaacaatt ccttatttcc
     2701 ggcatcatcg ggtcactggt gctttgcggt attgcgttta tgattttgat ttattctact
     2761 ccgggaatga gttcaccttg gcgatatgtt gttcaggcat tgcaggtaat tattattttg
     2821 ttctccattc ccaatgccat tttgggtatt cgccgactta ttggaagtag aatcggattg
     2881 cgcatatctg atgaaggtgt ttctgatatt actgttgcta atgcggggag gatgtattta
     2941 tggaaagaaa tcaaggcaat cagattacat gatgctaacc aagtaaaaat tcctttcatt
     3001 gcaatcgaat tactggatcc ggatattttc atgcatggaa aaatcggcgt tgcaagaaaa
     3061 atattgcaaa ccaatattgc caagtatggt actcctgtgg taatcaggcc tacagttttg
     3121 gatatcgacc ctcatatatt gttagatgcc ttgcgtgaag gtttggatga attcacaacc
     3181 tcgcaggttc acaatgaaca ggccgaagta attaattaat gtcgggaact aatttgccga
     3241 taaaagcttt tcttctctca tgatagaaat tggtattgat agttttgctg ctaaaccttc
     3301 acgacttggc atttcagatg cccaggctat aaatgaatta ctggagcgca ttgtttatgc
     3361 cgacgaagtg ggattagatg tattcggatt aggggagcat tatagaaaag aatttttgga
     3421 ttctgcatca tacatgctac tggcagcagc ggcatctcgc acaaaaaata taaaactgac
     3481 aagtgcagtt acggttttaa gcgcagcaga ccctgtcaga gtatttcaaa attttgcaac
     3541 tctggatctc atttcaaatg gaagagcgga aatagtgacg ggccgtggat cttttatcga
     3601 atcttttcct ttgttcggat atgacttgca tgattatgat gccttgttta ccgaaaaatt
     3661 ggatttactg ctgaagattc gcgataatga atttataacg tgggaaggtg cgttcagacc
     3721 tgcgcttcaa catcaacctg tttatccgcg accaatgcag gaaaagttgc caatatggat
     3781 tggtgttggc ggaacaccgg cttcatttgc acgcgccggt gcattaggat tgcctttgat
     3841 ggttgctatc atcggtggtg aaacacgcag atttaaacca ttggtcgacc tgtataggca
     3901 agccggagca aaagccggat ttgcaccgga ggaattgcag gttggattac attcacttgg
     3961 ttatgttgca aataccaagg aagaagctat cgccgactat tatccgggtt acgcagaaat
     4021 gttcacgaaa attggcattg aaagaggttt tggtcctgtt acaaggggcg gattcgatgc
     4081 ccaaaatggt cactatggtg cattgttggt gagtgatcct gaggaggtgg cgcacaaaat
     4141 cattcgccat agcgagtcat taggtggtat aagcagattt acgtttcaaa tggatgccgg
     4201 tatcccacat gaaaaactga tgcaagcaat tcatctcatc ggcacaaaag tgaaaccgat
     4261 tttgatggat gctaaatgaa gtatgaaaac gaaaatgtaa taattgttgt ttacaccaaa
     4321 tgctaaagca gccccttctt ttcttttaaa gcatcgcgtg cgtcgcgaag aaagtctgaa
     4381 gcgaaaataa aggagttgag gttttcatct ttagcttgta aaatctcatc tttattccct
     4441 tcccaatctt taacaccttt ataaaggaac acgatctttt caccaatttc catcacacta
     4501 ttcatatcgt gagtattaat gatggtggtc atattatatt ctttggtgat ttcaatgatt
     4561 aaatcgtcaa tcacaattga agtgcgtgga tcgagacctg agttaggctc atcacaaaat
     4621 aagtatcgtg gatttaaaac gattgcacgc gcaataccaa cacgtttttt cattcctccc
     4681 gataattcac caggatactt tttatttgca ccggcaagat ttacacggtc gaggcaaaaa
     4741 ttcactctat cgagtctttc cttttgtgtg tcattgctga acatatttaa cggtagcatc
     4801 acattttcct caacagtgag gctgtcgaat aatgccgaac cctgaaataa catgcctatt
     4861 tctctgcgca ataatttttg ttcggaacgc gacaagtcat aataatctct gtcgttaaaa
     4921 aatactttgc cggcatcggg tgtgatgagt cctgtcatga tcttcatcag caccgttttt
     4981 ccggatccac tgatacctat aaccaggttt gtttttcccg attcaaaaac ggcagaaact
     5041 tgcttcagca catgctgttc accaaatgat tttgatatgt cgcgaatctc tatcataata
     5101 acaacgccgc aatcacataa tctgccaata aaataaatat gcaactataa acaacagctt
     5161 ttgtgctggc gcgaccgatc tctaccgagc caccattcac atagtagccc atatagcagc
     5221 tgatagaaga gacaataaat gaaaaagcaa aggatttaat gaacatcaac tgcacatcaa
     5281 aaggatcgaa atacattctg atacctctca cataatcttc gacagaataa tttgaaccgg
     5341 ctgctgtagc gatataacct cccataattc cggagtatgc tgctacggaa accaaaattg
     5401 gaatggtaat gatggctgcc atcaccctgg tgcttatcag gtaagattct gtatttacac
     5461 ccatgatttc taaagcatcg atctgttcag tcacacgcat tgatcccaac tcagatgcca
     5521 gattagaacc aacttttcct gcgagaatta atgcggtgaa tgtcggcgcc aattcaaaaa
     5581 tgatggtatt gcgcactatc aatccgatat atgatgaagg aacaaaggga gtaccatata
     5641 attgatatgc cgtttgcaca gcagttaccg caccaataaa aatggaaacc aataaaacaa
     5701 taaatgctga accaacacca atatccacca tctgcctgaa catttcctta aaatacattc
     5761 gccaacgttc agggcgcgaa aacatccttc cgatcataat cagaaatttg cccagatgtt
     5821 ggaaaatatt gaattcaatg ttcattgcct caaaattata cttttattac ttcacagact
     5881 acatatctat gtgctaattt agaatgcctc tctgaaggtt acatataaac tgccatttcc
     5941 ttcttcgtca aatccataat ctactcgtaa actgagtttg tcctcgggca caataaacaa
     6001 tcgtccgccc acaccatacg aataatgcaa acctgctgtt gaaaatgaat tgagatcggg
     6061 agctacctca cctgtacccg caaaaatgtt caccccaaaa tatttccaga aagtaatgcg
     6121 gtattcagct tgtacggcaa tgtagttgat atcgcgatac cgtccttcat aatgtcctcg
     6181 catatgcctg tcgccgccca attgcgctaa cctgttgaaa ggggcttcac ccggaacaaa
     6241 ttgtgcatat acattgaatc cgagcacatc atcgcgcatc attttgttga actggctgaa
     6301 ttcgaacatg taatctacat aggtataatt actgccggtt agtttcgaat gaaatactgc
     6361 cgagaaatca gcattcaggc cttttaaagg gaaataaata ttgtctcgcg tatcatatgt
     6421 caaccatgga ccaatacctg tgttcaaacc accatcttca cccggaacat cttcggtatc
     6481 aaaaatgcct ccttccttaa catcataaac cttagtggtt tctataaatg tcttcagtcc
     6541 cgcaaagaat tttggaaaaa ccaatcgctc accattcacc tgcaagttga agtaatcata
     6601 attataagat tccttatcgt ctggtgaatt ttgattgccg attccgtaaa aggcgtcagg
     6661 gaacttgaaa aagtttgttg tgtagttcag tttatacaag ttatccccga agtaactctc
     6721 gccactgata ttgctttcaa tctgcttatt aagcgtataa acaccaactg ccttgatcac
     6781 tgatgcgttg ctgtttggat acttttttga tggtggtaga taccatacca gtccggcacc
     6841 gaaatacaat cgagtctcag gcgaataacc caataccggc gaaggaaaaa acttgccttt
     6901 gctgcggtgt acggtatctg tgtgtgttga atccacctgt gccattcctt gaaagtgcat
     6961 gctacacaac gcaaccataa gtcctgcaaa gcggaaaaat tggcgacaag ggctggggga
     7021 gaggagcatc aaaggttgat aatggctaaa taaacgtgaa ccggttgttg aatcttattg
     7081 gcgatgcaaa attgcagttt ttaaagggta aatgacagaa atccttcaac atgtatacgg
     7141 aacgctgagc cccatggatg cggtctgacc ttcattttcc cgaaaatcca cattaaaacc
     7201 ctaaatatgt gggtataaaa cgccgcaatc tttgacactt tcagagttta cccctatctt
     7261 tgcggcggat tttaaaaccg tataataaag tctacaaatc atgtccggta aaggaaaaat
     7321 caagcagatc atcggcgccg tagtcgatgt agctttcgaa gaaggtagtc tcccagagat
     7381 cctcaacgcc ctagaggtga aacgtgaaaa cggtgaagcc ctctatttag aggttcaaca
     7441 gcaccttggt gaagacagcg tgcgcaccat cgctatggac tcaacagatg gtcttgtgcg
     7501 tggtgccgag gttatcgact tgggaaaaaa tatttcaatg cctacaggcg atgccatccg
     7561 tggccgcttg tttaacgtgg ttggaactgc aatcgacggt atgcgtccgg ttgactctga
     7621 cgtgcgttat cctatccacc gcaaaccgcc actgtacgaa gagctgtcaa ctgaagctga
     7681 ggttcttttt acaggtatta aagttatcga ccttattgaa ccatacgcaa agggcggtaa
     7741 aatcggtctg ttcggtggtg ccggtgtagg taaaacggta ttgatcatgg aattgatcaa
     7801 caacatcgcc aaaggatatt ccggtatgtc ggtgtttgca ggtgtgggcg agcgtactcg
     7861 tgaaggtaat gacctgctcc gtgaaatgat cgaatcaaac gttatccgtt acggcgaaga
     7921 tttcaaacat tctatggaac aaggtggttg ggatcttagc aaagtagatt atagccaatt
     7981 aaatcaaagc caggcaacgc ttgtattcgg acaaatgaac gaaccaccgg gagctcgtgc
     8041 tcgcgtggcg ctttccggtc ttactgttgc agaatatttc cgcgatgggg atccaaatga
     8101 ggctaaagga cgtgatatcc tgttctttat cgataatatc ttccgtttca ctcaggcagg
     8161 ttctgaagta tcagcccttc ttggtcgtat gccttcagcc gtaggttacc agcctactct
     8221 ggcatctgaa atgggtctca tgcaagagcg tattacctca acaaaacgcg gttctattac
     8281 ctctgttcag gcggtttacg ttcctgcgga tgacttgact gacccggctc ctgcaacaac
     8341 attctcgcac ttggatgcta ctacggtatt gaaccgtaaa atctccgagt taggtatcta
     8401 tcctgcggtt gacccactgg attctacatc acgtatcttg agtgccgata tcgttggtga
     8461 agaacattac cgttgtgctc agcgcgtgaa aggtatcctc cagcgctaca aagaattaca
     8521 agatatcatc gccatcctcg gtatggaaga attgagcgaa gaagataaac tcgttgtaag
     8581 ccgcgctcgt cgtgtgcaac gcttcctatc tcagccattc cacgttgccg aacagttcac
     8641 aggtttgaaa ggcgtgctcg taccaatcga agaaactatc cgcggattca atatgatcat
     8701 ggatggtgaa gtagatgaat accctgaagc agcattcaac ctcgtaggta ctatcgacga
     8761 cgctatcgaa aaaggtaaaa aattattagc tgacgcgaaa aaataaagga ggagttgagg
     8821 gttataggtc gagagttgag ggttgtattt cactcaccat tcacccacta acccactcac
     8881 ccactcaccc actcacccac tatgcaacta gaaatattat caccggaaaa aaagatctac
     8941 agcggagaag ctgtaggcgt gcaaatgccc ggcatcacag ggtcttttga agtgctgaac
     9001 aaccacgcac caatgatcgc tgcactggct aatggcagga tgcgcgtaaa taccggcaaa
     9061 gacaatatcg tctataatat caaaggcggc tttgttgaag tagtgaataa taccgttagc
     9121 gt
//
LOCUS       DAOQOU010000137         9019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4172_length_9019_cov_7.133088, whole genome shotgun sequence.
ACCESSION   DAOQOU010000137 DAOQOU010000000
VERSION     DAOQOU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4172_length_9019_cov_7.133088"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1597
                     /gene="creD"
                     /locus_tag="PLM90_11140"
     CDS             263..1597
                     /gene="creD"
                     /locus_tag="PLM90_11140"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HMZ89976.1"
                     /translation="MQENPSPGAFEQLNNWFTRSITVKLIIIVFLALILLIPASMVQG
                     IINERESKQSYAAESISNEWASEQNVAGPVLTVPVAVINPDGSKQWTNYVHLLPDDLV
                     IDGNVAPQSLHRGIYDAVVYTTALDIKATFDLSKLNSEIQDIKGTPDWQKAYFAVGIS
                     DLRGVKDNILVAVNNNNITAKAGVAKESPIHHGVSVPFKDVKIDSNGKFTVQYKLALQ
                     GSQMLEFQPLGNTTNIEIRSKWTNPSFTGAFLPDERVINDQGFAANWRVLELNRNFPQ
                     TWKNDEYNIGLDESAFGITLMTGPDDYQKSMRSVKYAILTIGLTFLLFFLFEIMNKRR
                     MHPFQYILVGLALCLFYILLISLSEQMAFNLAYIISSMTIILMITLYSRTVFPKWRSS
                     GILAIILIALYTYLFVTIQISDYALLLGSLGLTLMLAVTMYATRKIKWYGNE"
     gene            complement(1763..2890)
                     /locus_tag="PLM90_11145"
     CDS             complement(1763..2890)
                     /locus_tag="PLM90_11145"
                     /inference="COORDINATES: protein motif:HMM:NF027711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5005 domain-containing protein"
                     /protein_id="HMZ89977.1"
                     /translation="MKKSIASQLLLSLLTVMLFATCGEIVPDKEALTQSPDDESMMMT
                     GVTTKPANKFNNMFNRYGNGWTGGDATYSVKLPDGRTVWMFGDSFLDTVYADNTRPLT
                     SPLIRNCFMVQNGTNMTTLCGNNIDDPEALVNTADPINEWYWPGDGTVIGDTLYVFMM
                     YFVRTGGGGFDFAYQRTDLVTFSLPTITEVNRTTVFTNEDIMWGADILEDGSYIYVYG
                     PESFTFTKYPHVMRFPAGNIRGTKEFWNGTTWVTTEPATTVGRMKRTNGLNIDVSAQF
                     AVFYSGGKYRMVTQDVLFGPNIYSWEASNPNGPWKIKQTVYTTPETGGDIWTYNAWVH
                     PQFTTADGYILLSYNKNSLNFLDLFGDATIYRPQFVYFKYL"
     gene            2981..3991
                     /locus_tag="PLM90_11150"
     CDS             2981..3991
                     /locus_tag="PLM90_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HMZ89978.1"
                     /translation="MRNVRIWLWTGVVMILIQVMLGGITRLTESGLSIVEWDVVMGSM
                     PPTSQSAWEEEFAKYQQTAQFEKVNTDFTVSDFKRIYWWEFIHRLWARTLGFVFLIPF
                     IWFWIKKQLNASWKKKLIFVFVLGGLQGFVGWVMVASGLDGDPWVDPSKLTIHLLLAL
                     TLYMYLIWLAQDTFGKIAHKPIAKGNMNWITALIFIQIGLGGIMAGTDAGRLFNTWPL
                     MNGAFLPDGAFSNLSQAEKLTQLTTEINFIHRTFALIVATAVIIYWYRNRHTGSQKLK
                     QLNLTMVLLVVLQCVLGVLTLLNGSLQIPVFLGVAHQITACILLATAVLIMHRVKHDG
                     AS"
     gene            complement(4156..5082)
                     /locus_tag="PLM90_11155"
     CDS             complement(4156..5082)
                     /locus_tag="PLM90_11155"
                     /inference="COORDINATES: protein motif:HMM:NF039745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6383 domain-containing protein"
                     /protein_id="HMZ89979.1"
                     /translation="MKGKFLIEQYACLAAGFLLLNKQSDSQVVYTDVEPDWIFQDDGD
                     FSYLDMNDDGINDFGFLKSNHIISTLSSPGLNTTWYYRIALWVGPAMVGPDIAGISAH
                     ASSAAGTYCFPYPLEKSNLINNDLSFQNCGFQLLASKTAEVYYGYSYTFLGIGDWHIG
                     IGEFNNLNLDSTRYIGVRFEDEDDCLHYGWIRCAVMDSIGKLVVMDYAYEMQCNKTIA
                     AGNTVSYVDINSETTDSGISIYAFDGKIYFHGNSAGKMVITVSDITGQIVANQCISGN
                     DKTINMNFEPKGIYVVNVVCNGKYFNKKVVID"
     gene            complement(5375..5740)
                     /locus_tag="PLM90_11160"
     CDS             complement(5375..5740)
                     /locus_tag="PLM90_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89980.1"
                     /translation="MTITDYAPQYGGLPLDTTFILYDFGLQVNYTVFYPIDHTPVMNE
                     SMIDSVLLNDGIYHQRYHFYETGAIEQVWLEGIGSENGIFGAFLPIAAYMNGSSWLEC
                     FKRGDTYVYGTDSCDFNLH"
     gene            complement(5737..5994)
                     /locus_tag="PLM90_11165"
     CDS             complement(5737..5994)
                     /locus_tag="PLM90_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89981.1"
                     /translation="MTLRGSWDDFGNSNLYGNIISPTEDILIDGLAFQKPSLTLNYTT
                     SSIFPGTTSADDTYIPLDTIAAIRVEDEKVYLRISTLSFLK"
     gene            complement(6166..6930)
                     /locus_tag="PLM90_11170"
     CDS             complement(6166..6930)
                     /locus_tag="PLM90_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ89982.1"
                     /translation="MKLVKYVFWDIARNKILIGYAMFLFAVSFGLFSFSDDPIKATSS
                     LLNIVLIVLPLFSVIFATSYYYNAYEFTTLVLGQPINRLHLFFCQFAGVCIAMTLAFI
                     MGCGIPIIMYMPNIIGIMLLLSGISLTLVFISLAFMAGVLARDKARGIGIALLLWFYF
                     AFLYDALVLMLLFAFSDYPMENTMMTLAFLNPVDLIRIIMLMQLDMAAIMGYTGAVFN
                     KLLGSSSGTILCFAAILVWIALPLTISALRFRRKDI"
     gene            complement(6917..7642)
                     /locus_tag="PLM90_11175"
     CDS             complement(6917..7642)
                     /locus_tag="PLM90_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ89983.1"
                     /translation="MIQIDHLSKSYKSVHALNDLSLTFTAGQSIALTGPNGSGKSTLM
                     KCLLGLVFPDKGNITVNNLRVDKGSEYRRFIGYMPQIVQFPENMRVRQLFKMMRDIRQ
                     HDGNYDEDLYTAFDIQGMADKYLGSLSGGMKQKVNAGLALLFNPKIIIMDEPTAGLDP
                     LSAELLKDKLRKEKEKGKLIIISSHILSDLEEITNHVLFLMDGKVICNKSLPELIAES
                     GTENLSRAIAKLMDPKIAGNETR"
     gene            complement(7639..8874)
                     /gene="nosD"
                     /locus_tag="PLM90_11180"
     CDS             complement(7639..8874)
                     /gene="nosD"
                     /locus_tag="PLM90_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HMZ89984.1"
                     /translation="MLRFIWIWILIHCSLGAQSKTIHVNNHKGLAGVQDAINIAQLYD
                     EILLEEGVYTGEGLYINKPIILRGNNNVILDGGEKVTILTIEGTAALVEGLTFRNTGY
                     SSVEERCAIKLLSARGCIITNNKIEHCCFGIMLMKSGHSIITDNNINATNIHEQRSGN
                     GIHGWQSDSLTIRNNNIQGHRDGIYFEFVTCTGVYNNKSINNLRYGIHFMFSNNDEYI
                     GNTFSDNGSGVAVMFSHHVNIRNNAFDNCMGGASYGILLKEINDSQIINNKFRHNTNG
                     IFMEGTNRITLQRNLFLSNGWACRVQASCSSIYLNENNFIGNSFDIATNGSLSINNFN
                     GNYWDKYEGYDLDKNHIGDIPFSPVSMYGVIVERMPYTILLYRSLMVYLLDRSEKVLP
                     GITSEQIKDKQPLMHAVNL"
BASE COUNT         2731 a         1699 c         1833 g         2756 t
ORIGIN      
        1 aacgctggcg cgttaatagg gaatgcaatg atattgcttt gcaatatttt aattcagaag
       61 cgtaatcagc tccctttcta tcgacgatag gagatggaga ggggccgggg gtgaggtggg
      121 ttcgcgcaaa gtcgctaagt ggcgaaggcg caaagtctgc tacacccgca cacccgaaca
      181 cattaaaaaa aacaatatct atcagaattc ttgcataatt tctaaataac aaagaacttt
      241 gtaaaacaaa gcaaatgaaa ctatgcaaga aaatccttct cccggcgcat ttgagcagtt
      301 aaacaattgg tttacacgct cgataacggt taagcttatt attatcgttt ttttggcgct
      361 tattctgttg attccggcat cgatggtgca aggtattatt aatgaacgag agtcgaagca
      421 atcgtatgca gccgaatcta ttagtaacga atgggcatcg gagcaaaatg ttgcgggccc
      481 ggtgttgacg gttccggttg cggtgatcaa tcccgatggc agtaagcaat ggactaatta
      541 tgtgcattta ttgcctgatg atctggtgat agatggaaat gttgcacctc aaagcctgca
      601 tcgcggaata tatgatgctg tggtttatac tactgcattg gacataaaag caacattcga
      661 tttatcaaaa ctcaatagcg aaatacaaga tatcaaagga acaccggatt ggcaaaaagc
      721 atattttgca gtaggtattt ctgatctgcg cggtgtgaag gataatatac tggtagctgt
      781 taataataat aatatcaccg caaaagcagg tgttgcaaaa gaatcgccta ttcatcatgg
      841 cgtttcagtg ccatttaagg atgtgaaaat tgattccaac ggaaaattta ccgtgcagta
      901 taaactagca ttacaaggta gccagatgtt agaattccaa cctttgggaa atactaccaa
      961 tattgaaatc agaagtaaat ggaccaatcc aagttttacc ggtgcatttt tgccagatga
     1021 acgcgtgata aatgaccagg ggtttgcagc aaattggaga gttttggaac tcaacagaaa
     1081 ttttccgcag acatggaaaa atgatgaata taatattggt ttggatgaat cggcctttgg
     1141 aattacatta atgaccggac ctgatgatta tcagaaatct atgcgaagtg taaaatatgc
     1201 cattttaaca atcggtctca cattcttact tttcttcctg ttcgaaatta tgaataaacg
     1261 tcgcatgcat cctttccaat atattttagt tggattggca ctatgtttat tttatatttt
     1321 gttgatatca ttatcggaac agatggcatt taatttggca tatattattt cttctatgac
     1381 cataatatta atgatcacct tatattcaag aacggtgttt ccaaaatggc gatcatcggg
     1441 aatattggcg atcatactta tagcattgta tacctacctt tttgttacca tacagattag
     1501 cgattatgct ttattgctcg gtagtctcgg actgaccttg atgctggcag taacgatgta
     1561 tgctaccagg aaaattaaat ggtacgggaa tgagtagaga ggaaaaagga gaaaggaaaa
     1621 aggagaaagg aaaaaggaaa aaggaaaaag gaaaaagctc tgcgtctctg cgcctctgcg
     1681 tgaaaccaac cctccgtatt acataaaccc cataaaaaaa gggatctgca tattgtagat
     1741 cccttttttt taatggtttt ggttagaggt atttgaaata aacaaactgc ggtcggtaaa
     1801 tggtggcatc gccaaacaga tcaagaaaat tgagactgtt tttattatac gacaataata
     1861 tataaccatc ggcagtagta aattgtggat gcacccatgc gttgtaagtc catatatcgc
     1921 cacctgtttc gggcgttgta taaacagttt gttttatttt ccatggtcca ttgggatttg
     1981 atgcttccca ggaataaata ttcgggccaa acaatacatc ctgcgtaacc attcgatatt
     2041 tacctcctga ataaaataca gcaaattgtg cagaaacatc aatatttaat ccattagtgc
     2101 gtttcattct gcctactgtt gtagcaggct ccgttgttac ccaagtagtg ccattccaga
     2161 attcctttgt tcctcgaata tttcctgccg gaaagcgcat cacatgcgga tattttgtga
     2221 acgtaaatga ttcaggtccg tatacataaa tgtagctgcc atcttcaaga atatctgcac
     2281 cccacataat atcctcattt gtaaatacgg tagtgcgatt aacttctgtg attgtcggaa
     2341 gtgaaaatgt aaccagatct gttctttgat atgcaaaatc aaaaccaccg cctccggtgc
     2401 gaacaaaata catcatgaaa acatacaatg tatcgccaat aacagtgcca tcacccggcc
     2461 aataccattc atttatgggg tcggcagtat tcaccagcgc ttcgggatcg tcgatattgt
     2521 tgccgcacag cgtggtcatg ttggttccgt tttgtaccat gaagcagttg cgaatcaaag
     2581 ggcttgttaa tggacgcgta ttatcggcat aaacggtgtc gagaaatgaa tcgccaaaca
     2641 tccaaacagt tctgccatca ggcaatttaa ccgaataagt agcatcgccg cctgtccacc
     2701 cattaccgta gcggttgaac atattgttaa atttgtttgc cggtttggta gtaacgccgg
     2761 tcatcatcat agactcatcg tcggggctct gagtgagcgc ttccttatcg ggaactatct
     2821 caccgcaagt ggcgaaaagc atcacagtca gcaagcttaa gagtaattgg gaggcaattg
     2881 attttttcat tggtgtgatt cttatctatc aaagttaaac aaaggaaata aatatgtgtt
     2941 aattgaactg atgtaagcta cttttgcaaa aaaatccgat atgcgcaatg ttcgaatctg
     3001 gctttggacg ggtgtggtaa tgatattgat tcaggttatg ttgggtggta tcacgcgatt
     3061 aacggaatcg ggcttgtcga tagtagaatg ggatgtggta atgggcagta tgccgccaac
     3121 atcgcaatcg gcatgggagg aggaatttgc caaatatcag caaacggcgc aatttgaaaa
     3181 agttaacacc gattttaccg taagtgattt caagcgtatt tactggtggg aatttattca
     3241 tcgcttgtgg gctcgcacgc ttggctttgt atttttaatt cctttcatct ggttttggat
     3301 taaaaaacaa ttaaatgctt cgtggaagaa aaaactcata ttcgtttttg ttttaggggg
     3361 attgcaggga tttgtcggat gggtgatggt agccagcgga ttagatggcg atccatgggt
     3421 agatccgagt aaactcacca tacatttatt actggcattg acattataca tgtatttaat
     3481 ttggcttgca caagatacgt ttggtaaaat tgcgcataag ccaatagcta aaggaaacat
     3541 gaattggatc acagcattga tatttattca aatcggattg ggtggaatta tggcaggcac
     3601 cgatgcaggc agattattta atacatggcc cttgatgaat ggtgcatttt tgcctgatgg
     3661 cgcattttct aatttatcgc aagcagaaaa actgacacag ctgacaacgg aaataaattt
     3721 catacatcgc acatttgcat taattgtggc aactgccgta attatttatt ggtatcgcaa
     3781 tcgacatacc ggttctcaaa aattgaagca gctgaatctt acgatggttc tgcttgttgt
     3841 actacagtgt gtattaggtg tgctcacatt gctgaatggc agcttacaaa ttcccgtttt
     3901 tctgggtgta gcgcatcaaa ttacagcatg catattatta gcaacagcag tgttgatcat
     3961 gcatagagtg aagcatgatg gggcatcata aacataacat gaaatcggtc gcactatatt
     4021 ttgtcacctg cttcagattt acaaagaagc agtagtggat attgtaattt ttcaagggct
     4081 aaatatttcg cagtatttaa cttatcctca tgccgttatt cttcatctcc tcttcatatt
     4141 tttttttatt aaatatcaat ctatgactac ttttttgtta aaatattttc cattgcagac
     4201 aacatttacc acatagatgc ctttaggttc aaaattcata ttaattgttt tatcatttcc
     4261 ggaaatgcac tgattagcta caatttgtcc ggtaatgtct gagacggtaa ttaccatttt
     4321 acccgcggaa tttccatgaa aataaatttt accatcaaaa gcgtaaatag atatccctga
     4381 atcagttgtt tctgaattaa tatctacata tgaaacagta tttcctgccg caattgtttt
     4441 attgcattgc atttcatatg cataatccat aaccacgagt ttcccgatag aatccataac
     4501 agcgcatcga atccaaccat aatgcagaca gtcatcctca tcttcaaacc ggacccctat
     4561 atagcgggtt gaatctaaat ttaaattatt gaattctcct attccaatat gccagtctcc
     4621 tattcccaaa aatgtatagc tatatccata atacacttct gcggttttag atgctaaaag
     4681 ttgaaatccg caattctgaa aacttagatc gttattgatg agattgcttt tttccagagg
     4741 ataggggaaa caataggtgc ctgcagctga actggcgtgg gctgaaatgc cggcaatatc
     4801 aggacctacc atggctggac caacccatag tgcaattcta tagtaccatg ttgtatttaa
     4861 tcccggcgaa gaaagagttg aaatgatgtg atttgatttc agaaaaccga aatcatttat
     4921 accgtcatca ttcatatcca gatatgagaa gtcgccatca tcttgaaata tccaatccgg
     4981 ctcaacatct gtataaacca cctggctgtc actttgttta tttaagagca agaagcctgc
     5041 tgcaagacat gcatactgct ctattaagaa ttttcctttc attggaaatt atatcaagca
     5101 gcagatttta ttgaattact tattaaagct tcaactgttg cgaaaaagtc ggccctgatt
     5161 caacctgaat gactgcggta tatatgcctg ttatcaaatt gggaatggaa ataatttttg
     5221 aatccggtat ttcactatca aatacgcatt gtccttgtac attgaataag taaattcgcg
     5281 cctgcatagt tgccggtaga tttttaatat atatctgatt ttcccatacg tagcagctta
     5341 tcgtttcggg agtagattca tgaattgccg ttgtttaatg cagattgaaa tcacaactgt
     5401 cggtcccata cacataagtg tcaccacgtt tgaaacattc aagccagctg gaaccgttca
     5461 tgtatgcagc aatcggaagg aaagcgccaa atatgccatt ttcactgcct atgccttcca
     5521 gccatacctg ctctatggca cctgtttcgt aaaaatgata tcgttggtga taaattccat
     5581 catttaatag aacagaatca atcatggatt cattcataac aggggtatga tcaattggat
     5641 agaaaacggt ataatttacc tgcaatccaa aatcgtataa tataaatgtt gtgtctaatg
     5701 gaagacctcc gtattgtggt gcataatcgg tgatggtcat ttcagaaacg atagggtcga
     5761 aatacgcaaa taaacctttt catcctctac gcgaatagct gcaattgtgt ccagtggtat
     5821 ataagtgtca tctgctgaag tggtgccggg gaaaatggat gaagtggtat aattaagtgt
     5881 gagcgatggc ttttggaagg caagaccatc aatcaggata tcttcggttg gtgaaataat
     5941 attaccgtat aaattagagt ttccgaaatc atcccatgag ccgcgcaagg tcatccaggt
     6001 tgcattatta tttggaaatg aaatgggatc ctatgcctga atatgagtaa agggaataat
     6061 cgcaagtaaa acaatgtata tcgttttcat agtaggtggt ttagtataaa gatagggaat
     6121 tgtcctactt ggttgcgaaa aggtaagaag attaccgtag aagccctaaa tgtctttcct
     6181 tcgaaaccta agcgcgctga ttgtaagtgg cagtgcaatc cacacaagaa tagcagcaaa
     6241 gcacaatatt gtacctgagc ttgatcccaa caatttattg aatacggcac cggtataacc
     6301 cataattgcg gccatgtcga gttgcatgag cataatgatt cttatcaggt caacgggatt
     6361 caaaaacgcc agtgtcatca tggtattttc catgggataa tcgctgaatg caaaaaggag
     6421 catcaggaca agcgcgtcgt aaagaaatgc aaagtaaaac cagagaagaa gtgcaatacc
     6481 tatacctctg gccttgtctc ttgccaaaac tcctgccata aaggcgagtg aaataaatac
     6541 caatgtcagc gaaatgccac ttaacaaaag catgatgcct attatatttg gcatatacat
     6601 tataatcggg ataccgcaac ccatgataaa tgccaatgtc attgcaatac aaacgcctgc
     6661 aaactgacaa aagaataaat gcaatcgatt tattggttga ccaagcacca gtgttgtaaa
     6721 ttcataagca ttgtaataat agctggttgc gaatataaca ctgaataatg gcaagacgat
     6781 taatacaata ttaagaagac tgcttgttgc tttaatcgga tcgtcactga atgagaataa
     6841 cccaaagcta acagcaaata aaaacatggc atatccgatt aggattttat ttcgtgcgat
     6901 atcccagaat acatatttaa cgagtttcat tgccggcgat ttttggatcc attaattttg
     6961 caatagcgcg actaagattt tctgtgccac tttcagcaat gagctccggt aatgacttat
     7021 tacaaattac tttaccatcc atcaaaaata atacatggtt ggtgatttct tcaaggtctg
     7081 agaggatgtg tgaggaaata attattaatt tccctttttc tttttctttg cgtagtttgt
     7141 cttttagtaa ttctgccgac aatggatcta aacctgcagt gggttcatcc ataataatga
     7201 ttttggggtt gaagagaagg gctaaacctg catttacctt ttgtttcata cctcctgaaa
     7261 gtgaacccag atatttatcg gccatgcctt gtatgtcgaa ggcagtatag agatcttcgt
     7321 cataatttcc atcatgctgc ctgatgtctc gcatcatttt aaataactgc cgaacgcgca
     7381 tgttttctgg aaactgaaca atctgtggca tatagccaat aaatcttcga tactcagaac
     7441 ctttatctac ccgcaggtta ttaacagtaa tatttccttt atcgggaaac accaatccta
     7501 agagacattt cattaatgtc gactttcccg aaccgtttgg accagtcagg gcgattgact
     7561 gcccggcagt gaaggtcagt gacagatcgt tgagtgcatg aacactttta taggatttac
     7621 tcagatgatc aatttgtatc ataagtttac cgcatgcatg agcggctgct tgtctttaat
     7681 ttgttcactg gtgattccgg gaagcacttt ttcagatcgg tcgagtaaat acaccataag
     7741 actgcgatag agtaaaatgg tatagggcat gcgttcaact attacaccat acatactaac
     7801 aggagaaaag ggtatatcgc caatatggtt tttgtccaga tcgtaacctt cgtatttatc
     7861 ccaatagttg ccattgaaat tgttgatgct aagcgatcca ttagttgcta tatcgaagga
     7921 gtttccaatg aaattatttt cgttcaaata tattgaagaa caactggcct gtactctgca
     7981 agcccatccg ttgcttaaaa ataaatttcg ttggagggta atgcgatttg taccttccat
     8041 aaagatacca tttgtattgt gtcgaaattt attattgatg atttgtgaat catttatctc
     8101 ttttagcagg attccgtagg aagcgccacc catgcaatta tcgaaggcat tgtttctgat
     8161 atttacatga tgtgaaaaca tcacagccac tcctgaacca ttgtctgaaa aggtatttcc
     8221 gatgtactca tcattattcg agaacataaa gtgtatgccg tatcgcaaat tattaatgct
     8281 cttattattg taaacaccgg tgcatgtaac aaattcgaaa tatataccat cgcgatgacc
     8341 ttgaatgtta ttatttctga ttgtgagact atcactttgc catccatgga tgccgttgcc
     8401 gcttctttgt tcgtggatgt ttgtggcatt gatgttgtta tctgttataa tgctatgacc
     8461 tgatttcatc agcatgatgc cgaagcagca atgttctatt ttgttattgg tgatgatgca
     8521 gcctcttgct gagagaagtt taatcgcaca acgctcttca acactgctat aaccggtatt
     8581 tcggaaggtt agtccttcca caagggcagc ggtgccctcg attgtaagaa tggtaacctt
     8641 ttcgcctcca tccaatataa cattattatt accgcggagt attattggtt tattgatata
     8701 tagaccttct ccggtataaa caccttcttc aagtagaatt tcatcataga gttgagcgat
     8761 attaatggca tcttgtacac ctgctaagcc tttatggttg tttacatgta tggttttgct
     8821 ctgtgcgcca agggagcaat gaatgagtat ccaaatccat ataaaacgca gcataaccaa
     8881 atataattaa ttattgcagc aattgttgaa tggtaagcga attgccttgc atttcctgtt
     8941 gaatacgttt tagggcgccg ctatcggcaa ggcctactgt tttgccgttc attggtgtat
     9001 gcaatgattc tgatgtaaa
//
LOCUS       DAOQOU010000138         8887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4258_length_8887_cov_5.300385, whole genome shotgun sequence.
ACCESSION   DAOQOU010000138 DAOQOU010000000
VERSION     DAOQOU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_8887_cov_5.300385"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..2813
                     /locus_tag="PLM90_11185"
     CDS             294..2813
                     /locus_tag="PLM90_11185"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ89985.1"
                     /translation="MKKILLLVYLSGIICAMQAQIVINEYSASNWKQFVDNHNDYEDW
                     IEIYNAGDMILNIGGYHISDNADNITKWTFPSPTYITPHGFIRLWCSGRDTIQGDNYH
                     LNFKLTQTKNSPDQIIVSNAAGAILDSLTIQLTLTHHSRARSTDGATEWKICTAPTPN
                     ASNNTTVMYNAYTSRPDFSIAGGLFDAPLMVGISPPDVNCTIHYTLDGTEPKQDDPVY
                     TDSIFIASTTVLKARSFSSDPDILPGKLEFMTYFVNVHHTLPIVSIAGDTLLELANGD
                     ADLEPIGSIEVFGVDGDSKTRSYGELNSHGQDSWANDQRSIDWVSRDEFGYSRALKEK
                     FFAQSDREDFQRMIFRAAGDDNYPAAHHTANLGSTHLRDDYIQMLAKEDNLDLDVRTS
                     ARCIVYLNGQYWGVYSFREKTDDHDFTDFYYNQGKYDIQYQMTWGNTWSEYGGSATIN
                     AWQDLRDLAELQDLSLDTNYHEITELLDITSLVDYFCVNTSAVCKDWLVYNTAVWRGF
                     NPEGTHHKWGYTLWDLDATFGFYINYSGVPDIGPTAEPCDIVDYDPDFFPPAQFDGKG
                     HVRLLEDLRENAEFEQFYLSRYTDLYNTTFNCENMLFKLDSMIAIIEPEMEMHANRWY
                     GTYDEWYSNYQDLRTWISMRCEDLYTGMSTCYSLTGPYEVTFNVEPAEAGYLKVNTLE
                     HHVLPWQGTYFGGMENKIIAYANDPDIWAFDHWEAGTTTFSTTWDSTMATLTSDDNIT
                     AVFRLKTDINDNEQMAFAVYPTIFEDALMLNYTSTDGSDVTFTMYDTHGRFVASKIFA
                     APTPGGYAESWDVSSLQLPAGLYLIECVSGDQRGSVKVMKQ"
     gene            2985..3479
                     /locus_tag="PLM90_11190"
     CDS             2985..3479
                     /locus_tag="PLM90_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMZ89986.1"
                     /translation="MQLDDLDYQILEQLIHDGRKSFTDIADELSVSVGTVRNRYNTFI
                     ENGLLNIIGRVHPEKIGFNTYAQILIKVRPVEKISAVADNISQLPEVSFLAMTTGAYD
                     LEVNVMCRNNEHLVELMTNHITKMDGIFESSTNIYFKVYKYAQPDLYLLRREGSAIDI
                     NNEK"
     gene            3529..6636
                     /locus_tag="PLM90_11195"
     CDS             3529..6636
                     /locus_tag="PLM90_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ89987.1"
                     /translation="MHKPTTQFKFVLVIACWCIAFQLQAQTIITGVVRDSSTNETLIG
                     ANVRLVSDPSVGTSTDLDGAFTLSLQTSSGALKISFVGYNEQTISFNGSGTLNVLLSS
                     EHILEEIVVVGYGTQKKSDVTGSISSTRNEDFRDQPISNVAGSIQGKVSGIDIRNASG
                     TPGAGLLVSVRGQYNPLYVVDGIPMISESNSSISTSFDLQGNSVGAGQNISSISDINP
                     DDIESIEILKDASAASIYGARAANGVILITTKRGAAGKTRFSLNYYSGIQQVSRPLDF
                     LSSEEFVDLINEARQNDLNAYEADPSLFGDDYDPALSTNPLPDSWATGVNTNWMDEIF
                     RVAPINNVEFSASGGNDKTKFYISNSYFDQQGIVIESYYKRFNSRINLDHQVSDKLKI
                     GENISLTTSGNRRSLNDNVYTGTVTNAIGASPLMPVYEDDGSYASFEDYQASWLSDNP
                     VLSTKEIIPFTRTNRALASIFADYAFTPKLHFRSTWSADVTFLTDDIYFSPITTDAEA
                     VGGKAMNSAFNNKVWLGENILSYNSELGNNNFLDIIGGFTLQVSNSDYVSISGQGFPA
                     GSGLQDVSSAAIVTGGESFSTGWSLVSFLGRANYTIKSRYLFSLSARVDGSSRFSPNN
                     QFGFFPAGSVGWHMSDEKFWPSGLFITDMKLRMSYGITGDQEIGDFQYISFWSPVGYN
                     GQAGLGPRNLADENLRWQSNKMFNTGLDYELYRGRISGSLEYYIGNKTDLLSDDVISG
                     VTGFGSVTRNYGNIQTSGFELSSNIYVYDRKDFDWTVGVNVSWLHNEIIDLSTDSILL
                     SAYSDLTATHILAEGQAYGSFWGVPYEGVDPETGDPIYTDTNGDGVIDDADAQILGKA
                     TPDWFGGLNTSLRYKKWDAQIAATFTLGNDVYNMIRSTYQTGGWSDYGWDEEYYLYQV
                     YANNTHVIDDRWQQAGDVTDIPRASLYFYNFYQNSGQAIESGSHARIREISIGYTIKP
                     SSIKSFESLRIYAQVQNAYVFTKYTGFDPEVSSTGGDHPETAGVDYAAYPQPRTYMVG
                     FNFKF"
     gene            6653..8047
                     /locus_tag="PLM90_11200"
     CDS             6653..8047
                     /locus_tag="PLM90_11200"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ89988.1"
                     /translation="MKKIFYFIIAAMALTGFNSCEGFLSQTSTNEVADSDVFTDAAGL
                     ENARIGLYNCLQSQYYYGGYYQLIADAYSDNGTTGGYDNLDLDELGYKEASADNIYIE
                     NVWIAIYNTIYTANQILANIDNIQDETLSDEVSDDIKGEALAIRAMAHFDLLRMFGEH
                     WDLSSSIGIPVVTEVLSTDAQMKRNTVSETYNAILADLITAESMLKNDDYRYDPPIFK
                     GPQFMTQTACQALLARVYRSMGDMSNALDKASEVINTGFASMIDGDQVSDIYNTKFSS
                     EAIFELAFNAQDKSQFNALTYAREDALRSEVSFLAAADLNDFFAGRPDDFRAPLLNFT
                     GNDVSIEPDGRTEKYRGEIFQDNSAFIMRAAEMYLIMIEAKGYPAGVTDMISFTLNRG
                     VTSVDLTSSEAFEQALMNERRAELNFEGDRYFDLAHFGKVSEILGDEVLPCLPIPNRE
                     IGANGGLLEQYPGY"
     gene            8074..>8887
                     /locus_tag="PLM90_11205"
     CDS             8074..>8887
                     /locus_tag="PLM90_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ89989.1"
                     /translation="MTNSFRSIHPFDQHVIAEYPLMDAKAVDTVLMHSADAFKHWRRT
                     SMQLRADLLLRLAVVLRENKDVYAKIISLEMGKLFKEAQGEVEKCAWNCVYYAENGAA
                     MINPEPAPADGSESYVVFEPIGAVFAIMPWNFPFWQVFRFAAPVLLGGNTIILKHAPN
                     VTGCAIAMQQIFLDAGFPEHVFQTVVIDVDLVENIIAHEIVQGVTLTGSERAGSAVAS
                     LAGKHLKKTVLELGGSDALIVLNDADVNLAAKVALQSRFQNAGQSCIASKRFI"
BASE COUNT         2669 a         1747 c         1890 g         2581 t
ORIGIN      
        1 tatcaggtat caagtatcag gtatcaagta tcaggtatta gaaaaaaaac tcaccactca
       61 ccattcggta gtgagtagtg agaaaattca cgagtcacga atcacgattc acccattcac
      121 cactcacctt ttttcaatca cggagttagc ggagtttgcg gagatgccac gaactgcctt
      181 tcggcgaggc aatttcacaa ttcaccactc accactcacc actcacccac tcacccactc
      241 acccactcac ccattttttc tttaaattta ccttaccaaa ttgtaacatt tacatgaaga
      301 aaatactcct ccttgtttat ctgtcaggca tcatatgtgc catgcaagcg caaatagtga
      361 tcaatgaata ctcagcttcc aactggaaac aatttgtcga taatcataat gattatgaag
      421 actggataga gatttataat gccggtgata tgatactaaa tatcggcgga tatcatataa
      481 gtgataatgc ggataatatc acaaagtgga cattcccttc gccaacttac atcacaccac
      541 atggctttat cagattgtgg tgttcaggaa gggataccat ccaaggcgat aattatcacc
      601 tgaattttaa acttacgcaa acaaaaaatt ctccggatca aattatcgtt agcaatgccg
      661 caggtgcaat tctggattcg ctgacaattc aattaacact aacgcatcat tcacgcgcaa
      721 gatcaactga tggtgctact gaatggaaaa tttgtaccgc cccgactcca aacgcttcga
      781 ataacacaac tgtcatgtac aatgcttata catcccgacc cgatttcagt attgcgggtg
      841 gtttgtttga tgcaccttta atggttggta ttagtccgcc ggatgtaaac tgcaccattc
      901 attataccct cgatggaaca gaaccaaagc aagatgatcc tgtgtatacc gacagtattt
      961 tcattgcatc aacaaccgtt ttgaaagcaa gatctttcag ttctgatcct gatattttgc
     1021 cggggaaatt ggaattcatg acatattttg taaatgtaca tcatacactt cctattgttt
     1081 caattgcagg tgatacccta cttgaattag caaatggcga tgcagacctt gaacctatcg
     1141 gatctattga ggtgtttggt gtagatggtg atagtaaaac gcgctcgtat ggtgaattaa
     1201 attcacacgg acaggattca tgggcaaatg atcagcgcag tatcgattgg gtgagtagag
     1261 atgaattcgg atatagtcgc gcattaaaag agaaattttt cgcgcaatct gatcgtgaag
     1321 attttcagcg aatgattttt cgcgcagcag gtgatgataa ctatcctgct gcacatcaca
     1381 ctgcaaattt aggaagtact catcttagag atgactatat acaaatgctg gccaaagaag
     1441 ataatttaga tcttgatgtg cgcacatcgg caagatgtat cgtgtatttg aatgggcaat
     1501 actggggtgt atattctttc agagaaaaaa ccgacgatca tgatttcact gacttttatt
     1561 acaaccaggg aaaatatgat attcaatatc aaatgacctg gggaaatacc tggagtgaat
     1621 atggtggttc tgcaactatc aacgcatggc aagacctccg cgatcttgcc gaattgcagg
     1681 atttatcatt agatacaaat tatcatgaaa taacggaact gcttgatatc actagtttgg
     1741 tagattattt ctgcgtaaac acctccgccg tttgcaaaga ttggttggta tataatacgg
     1801 cagtttggcg tggatttaat ccggaaggaa cacaccataa atggggatac accttatggg
     1861 acctcgacgc cactttcggc ttttatatca attattccgg agtgcccgac attggtccta
     1921 ccgctgaacc ttgcgatatc gttgattacg atcctgattt tttccctccg gcacaattcg
     1981 atggcaaagg gcatgtgcga ttgcttgaag atttacgtga aaacgcagaa tttgagcaat
     2041 tttatctttc acgatataca gatttataca atactacctt caactgcgaa aatatgctgt
     2101 ttaagctcga tagcatgatc gccattattg aacctgaaat ggaaatgcat gccaaccgat
     2161 ggtatggcac ttatgatgaa tggtactcga attatcagga tttgcgaacc tggatttcta
     2221 tgcgttgcga agatttgtat accggtatgt caacatgcta ttccttgacc ggaccttacg
     2281 aggtaacatt taatgttgaa cccgcagagg ccggatattt gaaggtaaat actttagaac
     2341 atcatgttct tccttggcaa ggcacttatt ttggtggcat ggagaataaa attatcgcgt
     2401 atgccaacga tcctgatatt tgggcatttg atcattggga agccggaacc acaacattca
     2461 gcacaacatg ggattctacc atggcaacac tgacatctga tgataatatc acagcagtct
     2521 tccgtcttaa aactgatatc aatgataatg agcaaatggc tttcgctgtt tatccgacaa
     2581 tatttgagga tgcgttgatg ttgaattata catccacaga tggcagcgat gttacattta
     2641 caatgtacga cacacatggt cgatttgtgg cttctaaaat atttgccgct ccaaccccag
     2701 gcggatatgc tgaatcctgg gatgtatcct cattgcaatt acctgcgggt ttatacctca
     2761 tagaatgtgt ttccggagat cagaggggaa gtgtgaaggt gatgaagcag tgattcctat
     2821 gtggttgcgg actgtttaaa taataaatat tgggatattt catatctcac ttgttgacat
     2881 gtgaaataac aatatatcaa ctttcaccct tttcaataaa aaaattacaa aaaaattagg
     2941 aaatattgaa ataatttcaa aattttgata ccagaaccta ctttatgcag ctagatgacc
     3001 tggattatca aatattggaa caactcattc atgacggcag aaagtcgttt accgacattg
     3061 ccgatgaact aagcgtatct gtcggcacag tgcgcaaccg ttataacacc tttatcgaaa
     3121 acggattact caacattatt ggcagagtgc atcccgaaaa gatcggcttt aatacctatg
     3181 cacagattct tataaaggtg cgtcccgttg aaaagatttc tgctgtggca gataatattt
     3241 cacaactgcc tgaagtgtca tttcttgcca tgaccacagg tgcctacgac ctggaggtga
     3301 atgtgatgtg tcgtaataat gaacatctcg tagaattgat gacaaaccat atcactaaaa
     3361 tggatggcat cttcgaaagt tcgacgaata tttatttcaa agtatataaa tacgcacaac
     3421 ctgatttgta tctcttaagg agagagggta gtgctattga tataaataat gaaaaataag
     3481 aacgcgaatt tcaacggatt aaaaaacata caaacccaaa ccctaatcat gcataaaccc
     3541 actacccaat tcaaatttgt attggtcatt gcctgctggt gtattgcgtt tcaattgcaa
     3601 gcgcagacta ttatcaccgg cgtagtgcgt gattcctcta caaatgagac tctgatcgga
     3661 gcaaacgtga gactcgtttc agatccttca gtcggtacat ccactgatct tgacggagca
     3721 ttcacgcttt ctctgcaaac gtcttccggt gcattgaaaa tatcgtttgt cggatataat
     3781 gaacaaacaa tttctttcaa tggttctggt acattgaatg tactattatc gtcggaacat
     3841 attctcgagg aaatcgtagt tgtcggatat ggcacacaga aaaaaagtga tgtcaccgga
     3901 tctatttctt ctacccgcaa tgaggatttc cgcgatcagc caatttcaaa tgtagccgga
     3961 agtatccaag gaaaagtaag cggtattgat attcgcaacg cttccggaac gccgggagca
     4021 ggattgctgg tgagtgtacg cggtcaatac aatccgctgt atgttgtcga tggaattccg
     4081 atgataagcg aaagtaattc aagtatttct acttcatttg atttacaggg aaacagcgta
     4141 ggcgcaggac agaatatttc atcgatatca gatattaatc ccgacgatat tgaatctatt
     4201 gaaatcttaa aagacgcatc tgctgcatca atttacggtg cccgtgctgc aaatggtgtc
     4261 attctgatca ccaccaaacg cggagccgca gggaaaacaa gattcagcct caattattat
     4321 tcgggaatac agcaggttag tagaccgctg gattttttga gcagcgaaga atttgtcgac
     4381 ctcatcaatg aagcgcgtca aaacgacctc aatgcctacg aagcagaccc atccttgttt
     4441 ggcgatgatt atgaccccgc cttgtcgacc aaccctttac cagactcatg ggcaaccggt
     4501 gtaaacacaa attggatgga cgagatattt agagtagcac ccattaataa tgttgaattt
     4561 tctgcatcag gaggaaatga taaaacgaaa ttctatattt caaactccta tttcgatcaa
     4621 caaggtattg ttatcgaatc atattataag cgatttaact cgcgcatcaa tctcgatcat
     4681 caagtaagtg ataaacttaa gataggtgaa aatatttcac ttaccacttc cggaaatcga
     4741 cgttcactga atgataacgt atataccgga acagtaacta atgctatagg cgcatcaccg
     4801 ttgatgccgg tgtatgaaga tgatggcagc tatgcaagtt ttgaagatta tcaggctagc
     4861 tggttgagtg ataatcctgt tctttctacc aaagaaatca ttcctttcac acgcaccaat
     4921 cgcgcacttg cctccatctt cgctgattat gccttcaccc caaaattaca tttcagatct
     4981 acatggtcgg cagatgtcac ttttttgact gacgatattt atttctctcc aattaccacc
     5041 gatgcggaag cagtaggcgg taaagccatg aatagcgctt tcaataataa agtttggctc
     5101 ggtgaaaata tattatctta taactccgaa ttaggtaaca ataattttct cgatatcatc
     5161 ggaggattta cattgcaagt atctaacagt gattatgtaa gtatcagcgg acaaggattt
     5221 cctgcgggaa gtggtttgca ggatgtatca agcgcagcaa tcgtcacagg tggagaatca
     5281 ttctcaaccg gctggtcgct ggtgtcattt ttgggaagag cgaattacac gattaaaagc
     5341 cgctacttat tttcacttag cgcacgtgtt gatggttcat cgcgcttttc accaaataat
     5401 caatttggat ttttccccgc aggatctgtc ggttggcata tgagcgatga aaaattctgg
     5461 ccttcaggat tgtttatcac tgatatgaaa ctccgcatga gctacggtat taccggcgat
     5521 caggaaattg gcgacttcca atatatttct ttctggtcac ctgtgggata taacggacaa
     5581 gcaggattag gtcctcgcaa tcttgctgat gaaaatctgc ggtggcaatc caataaaatg
     5641 tttaataccg gtctcgacta cgaattatat cgtggtagaa tttccggttc tttggaatat
     5701 tatatcggca ataaaacaga tttattgagt gatgatgtaa tttcaggtgt aaccggcttt
     5761 ggttcagtga ctaggaatta tggtaacatt caaacttcgg gatttgaatt gtcgtcgaat
     5821 atatatgttt acgaccgaaa agattttgat tggactgttg gcgtgaatgt aagttggttg
     5881 cataatgaga ttatagatct tagcacagac tcaatattat tgagcgcata ttccgattta
     5941 actgcaacac atattttagc tgaaggtcag gcatatggaa gtttttgggg agtaccttat
     6001 gaaggtgttg accctgaaac aggcgatccg atttatacag acaccaacgg cgatggtgtg
     6061 attgatgatg ccgatgctca aattttaggg aaggcaacgc ctgattggtt tggtggatta
     6121 aatacttcac tgcgctacaa aaaatgggat gctcaaattg ctgctacgtt tacgttaggc
     6181 aatgatgtgt acaatatgat cagatcaacc tatcaaacag gtggatggag cgattatggt
     6241 tgggatgaag aatattattt atatcaggta tatgccaata atacgcacgt gatcgacgac
     6301 agatggcagc aagccggaga tgttacggat atacctcggg catcattgta tttctataat
     6361 ttctatcaaa actcaggtca ggcaattgaa agtggctcac atgcacgtat tcgtgaaata
     6421 agcataggat atacgataaa gccttcttcc ataaaatcat ttgaatcatt gagaatttat
     6481 gctcaagtgc agaatgctta tgtattcacg aaatatacag gcttcgatcc tgaagtaagc
     6541 tctacgggtg gcgatcatcc ggaaacagca ggtgtcgatt atgccgcata tccgcaacca
     6601 cgcacctata tggttggatt taatttcaaa ttctaacgca aaagcattga tcatgaaaaa
     6661 gattttttat ttcatcatag ccgcaatggc attaacagga tttaattcct gcgagggttt
     6721 tttgtcgcaa acttcaacta acgaagttgc cgatagtgat gtgtttaccg atgctgcagg
     6781 attggaaaac gcgcgtatcg gtttgtataa ttgtttacaa agccaatatt attacggtgg
     6841 ttattatcaa ttgatagccg atgcctatag cgataatggc accacaggtg gatatgataa
     6901 cctcgatctc gatgaattag ggtataagga ggcttctgcc gataatattt atatcgaaaa
     6961 tgtatggata gcaatttaca atactattta tactgccaat cagattctcg ccaatattga
     7021 taacatccag gatgaaacac ttagcgatga agtaagtgac gacatcaaag gagaagcttt
     7081 ggcgataaga gcaatggctc acttcgattt attgcgcatg ttcggcgaac attgggattt
     7141 gtcgtcaagc atcggtattc ctgtggtgac agaggtttta tctacagacg ctcaaatgaa
     7201 acgcaatacc gtttcagaaa catacaatgc tattcttgcc gatttgatca ctgctgaatc
     7261 tatgctcaaa aacgacgact atcgctacga tcctcctata ttcaaaggtc cgcaatttat
     7321 gacgcaaaca gcttgtcagg cattactggc gcgtgtttac agaagcatgg gcgatatgag
     7381 caatgcactt gataaagctt cggaggtaat taacaccgga tttgcttcca tgatagacgg
     7441 cgatcaggtt tccgatattt ataatacaaa attctcttca gaagctattt ttgaattggc
     7501 atttaacgct caagacaaaa gtcaatttaa tgcgcttacc tatgcaagag aggatgccct
     7561 ccgcagtgag gtctcttttt tagctgccgc cgatcttaat gatttttttg caggaagacc
     7621 tgatgatttc cgggcaccat tgttgaattt taccggtaat gacgttagta ttgaacccga
     7681 tggaagaact gaaaaatacc gcggagaaat ttttcaggat aatagcgcct tcattatgcg
     7741 tgctgctgaa atgtacctca tcatgatcga ggcgaaagga tatcccgccg gtgttactga
     7801 tatgataagt ttcaccttga atcgtggggt tacctcggtc gacctaactt cctctgaagc
     7861 attcgaacaa gcattaatga atgaacgtcg ggcggaatta aatttcgagg gcgacagata
     7921 tttcgacctg gcacattttg gaaaagtatc tgagatatta ggtgatgaag tattgccttg
     7981 tttgccaatt ccaaacagag aaattggtgc aaatggcgga ctgcttgaac aatatcccgg
     8041 atactgatcg ttaacattaa taattaaaaa acattgacca atagttttcg aagtatacat
     8101 ccttttgatc agcatgtgat tgctgaatat ccactcatgg atgcaaaagc ggtggatact
     8161 gttttgatgc acagcgctga tgcttttaaa cattggcggc gcacttcaat gcaattgcgt
     8221 gccgatttat tgttgcgcct tgcagttgtg ttgcgtgaaa acaaagatgt ttacgcgaaa
     8281 ataatttccc tcgagatggg gaagctattt aaagaagcac aaggggaagt agaaaagtgt
     8341 gcatggaatt gtgtgtacta tgcagaaaat ggtgccgcaa tgattaatcc tgaacctgca
     8401 ccggcagatg gtagcgaaag ctatgtagta ttcgaaccta taggtgcagt ttttgccatc
     8461 atgccatgga attttccatt ttggcaggta tttcgttttg cagcaccggt attgcttggt
     8521 ggaaatacca ttatattaaa gcacgcacca aatgttaccg gttgcgctat cgccatgcaa
     8581 caaatatttt tggatgccgg attccctgag catgtttttc aaaccgtggt aatagatgtt
     8641 gatcttgtag aaaacatcat tgcacatgaa atcgttcaag gcgttacact aacagggagt
     8701 gagcgagccg gaagtgcagt tgcttctttg gcaggaaaac atcttaaaaa aactgttctc
     8761 gaactcggcg gaagcgatgc gcttattgta ttaaatgatg ccgatgtaaa tctggctgct
     8821 aaggtggcat tgcagagcag gttccaaaat gcaggtcaaa gttgcattgc gtcaaagcgt
     8881 tttattg
//
LOCUS       DAOQOU010000139         8689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4405_length_8689_cov_6.107135, whole genome shotgun sequence.
ACCESSION   DAOQOU010000139 DAOQOU010000000
VERSION     DAOQOU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4405_length_8689_cov_6.107135"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2561
                     /locus_tag="PLM90_11210"
     CDS             <1..2561
                     /locus_tag="PLM90_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ89990.1"
                     /translation="ANLALSKHVDEAVKILKAAHFDLIILETSGIGQSDTEITEHSDV
                     CMYVMTPEYGAASQLEKIDMLDFADIISINKFDKRGAQDALRDVRKQFQRNHKLFDKD
                     VETMPVFATIASQFNDPGTNQLYRHLMDTIREKTGVDLHSDFHVTDEMSKKIFIIPPQ
                     RTRYLAEISENNRGYDKKASEQSKIAQQLYALHLSEQSIDDASVKHVIQESYQKLLKK
                     LDHQHFDLIQQWDSKKKAYHDEFYIFKVRDKELKIKTHSESLSHLQIPKISMPRYEAW
                     GDILRWQLQENVPGEFPFTAGIYPFKREEEDPTRMFAGEGGPERTNKRFHYVSLGMPA
                     KRLSTAFDSVTLYGNDPEYRPDIYGKIGNSGVSICCLDDAKKLYSGFDLADPKTSVSM
                     TINGPAPMLLGFFMNAAIDQQCEKYIRANGLEAEVEAKIKAIYAGKEQLRPKYNAAEL
                     PQGNDGLGLMLLGVTGDQVLPADVYADIKAKTLSVVRGTVQADILKEDQAQNTCIFST
                     EFALRLMGDVQQFFISNKVRNFYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYL
                     SRGMHINDFGPNLSFFFSNGVDPEYAVIGRVARRIWAKAMKHKYGADDRTQKLKYHIQ
                     TSGRSLHAQEIDFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQL
                     IINKELGLAKNENPIQGSFIIEELTDLVEEAVLSEFDRITERGGVLGAMETMYQRGKI
                     QEESLYYETLKHTGEYPIIGVNTFLNSKGSPTVIPMEVIRATEEEKEAQISTLRNLQS
                     MQVEKAHHQLREIQLAAIENRNIFETLMEACKVCSLGQITKAMFEVGGQYRRNM"
     gene            2812..5244
                     /locus_tag="PLM90_11215"
     CDS             2812..5244
                     /locus_tag="PLM90_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ89991.1"
                     /translation="MRKLILALFLIPTCVFAQQNETVSASIDRTILDSLHMVLRAEIL
                     NLEAAKQKALAMGLPVSGSGEDGAFMSIRSLRNGQIMYYNTTNLNSAKTDGSNKLWSG
                     GGLGLSLNGSGIVARVWDAGAIKTTHQELNGRASMGVSQTATLSDHATHVSGTIAAFG
                     VDASAKGHANQAEIKGFDWDFDTDEMTTEAGNGMLVSNHSYSYIAGWYFNGGTGQWEW
                     YGDGTVSTVEDYIFGNYLDESRNVDLIVNASPYYLPVIAAANDRDDNVPTGATYKMIW
                     DGTIHTTDGTQPNPDGTYDCIPGGLQTAKNTLTVGAVNDIVNGYLAPGDVVLTGFTSY
                     GPCDDGRIKPDVVANGTSLHSCVSTSTTSYSDFSGTSMAAPSVTGSVVLLQQHHHNIF
                     GTYMLNATAKAVIIHTVDKASATFGPTYSYGWGLMNTDGAALLISDALIHPIRIQERN
                     LPNGGTYNFTGNSNGSEPLKITIAWNDPAASVPTPALDPVTKMLVNDLDLRVTRLSDN
                     TVFYPYKLNPASPGTAAITGDNNTDNVEYVYLATPSAGDYKITINHKGTLSGGDQDYT
                     IVVSGLSCIPYSLTENVTITAPATYTLPNGSIVSTSGTYTSNFTTSLGCDSTIITNLT
                     VNTFTCAVPTGLFTNSITSNSARVNWTAVPGATKYQIYYRPVGGATWIKKTATTNSKK
                     LTGLAANTTYEYKVKTQCDPYPASGFSALVNFTTLPLREGEITSESESSLVIYPNPAE
                     ENCTVSAEGIADGVYHMYIINLQGQKVVEQTILFSGDDFTVDVKNLASGMYNLVLSNN
                     NTHISDILVIAK"
     gene            5259..6473
                     /locus_tag="PLM90_11220"
     CDS             5259..6473
                     /locus_tag="PLM90_11220"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HMZ89992.1"
                     /translation="MALTDETLLKLETALSPLDSKKWQHTMSRVLRMILEFFFVALIV
                     SMFIGFLSMMSNGEMGDDMFFRVIRGFMLFVLAMVLFGIIRKTARDRFLSMQSAIAHQ
                     PWTLNLITIVFTVCAFAIGYFAGTAFLGGTFSPTFMIKWAGAIGSIFFLLGPYMLLKR
                     IEDNFTQQYKSILFANFLPGIAAQARYVMNEHIGTQTFTESKLFPYQEISSLKGSDLI
                     TRSQEQFQCSFLDVMQVERTQSNGKSETKYSTLFKGYLLSAAFNKSFTGETFVAPDAS
                     RELLGEVYGESLNELFHRPGTSIARMEDPDFEKAFSVYTTDQQEARYILSTKLIHRIM
                     ELKTYFVDDVYLSFVGNRVFVAVKSEKDIFSPPTIGNTYNKTLAEKQYAMLNALVTVP
                     ELFDLDRRIWAS"
     gene            complement(6512..6997)
                     /locus_tag="PLM90_11225"
     CDS             complement(6512..6997)
                     /locus_tag="PLM90_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HMZ89993.1"
                     /translation="MSENYEIDKVDIQILHLLMQDAMTPFTEIAQKIGVSPGTIHVRM
                     KKMDAMGLIKSSHLMVNPQKLGYGITAFLGVYLDKSSMYDSVVKELEKIPEIVDCHYM
                     TGTYSMFLKIICKDTNHLKKVLHDGIQPINGIDRTETSISLEESFSRPMKIELNGRKD
                     F"
     gene            complement(7255..>8689)
                     /gene="rlmD"
                     /locus_tag="PLM90_11230"
     CDS             complement(7255..>8689)
                     /gene="rlmD"
                     /locus_tag="PLM90_11230"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMZ89994.1"
                     /translation="SLREPNIVIMARNKKKIHQHVLIESVANSGSAFGRVEGKVVFTE
                     GVIPGDIVDIQVARKRNGYDTGYVTAIHKKSDLRIEPFCTHFDICGGCTWQNVEYETQ
                     LQFKQQLVDDAFRLLKRSPGFPEIEKIIASPYDRYYRNKMEYTFTNGAYIFDPHYDKS
                     NPPEKKPGCGFHVKGQFANVFDMERCYLQGSLSNEIRMAVRSYALMHGFAFYDLRNHT
                     GYMRNLIIRNTGTGDLMVILVVALDEPEKLLPMMEHIRNAFPEITSLYYCINTKHNDV
                     IYDIDVTLFAGKPHMIEEIDGIKYQLGPRAFFQTNTEQAKNLYRKTIAYAALKPDDKV
                     YDLYTGLGSIALQAANKCSKVVGVELVEAAIADAKENAKLNNIDNCTFIAGDMAKIFT
                     DDFIAEHGKANVVITDPPRAGMHKDVCLQLLKLAPERIVYVSCNPVTQARDIQLLIDD
                     YKVTALQPFDQFPQTYHVESIALLERR"
BASE COUNT         2586 a         1735 c         1863 g         2505 t
ORIGIN      
        1 aggcgaattt agctttgagc aaacatgttg atgaggcggt aaaaattctg aaagcagcac
       61 attttgatct gatcattctt gaaacatctg gtattggtca aagcgataca gaaattactg
      121 aacacagcga tgtgtgcatg tatgtgatga caccggaata tggtgccgcc agtcagctcg
      181 agaagatcga catgttggat tttgcggata tcatttccat caacaaattc gataaacgcg
      241 gagcgcagga tgccttgcgt gatgtgcgca aacaattcca acgcaaccat aaattatttg
      301 ataaggatgt agaaacgatg ccggtatttg ctaccatcgc atcgcaattc aatgatccgg
      361 gcacgaatca gttgtatcgt cacctcatgg ataccattcg tgaaaaaacg ggagtggacc
      421 tgcattccga ttttcatgta acggatgaga tgagtaaaaa gatttttatc attcctccac
      481 agcgcacacg ttatcttgca gaaatttctg agaacaaccg aggatatgat aaaaaagcct
      541 ctgaacaatc gaaaatagca cagcaattat atgccttgca tttaagtgag caatctattg
      601 atgatgcctc cgttaaacat gtgatacaag aaagttatca gaaattatta aaaaaacttg
      661 atcatcagca tttcgacctg attcagcaat gggacagcaa gaaaaaagcg taccacgatg
      721 aattttatat cttcaaagtg cgcgataaag aattgaaaat aaaaacgcat tctgaaagct
      781 tatcgcattt acaaattcct aagatcagta tgccgcggta cgaggcttgg ggagatattt
      841 tacgttggca attacaagaa aacgttccgg gagaatttcc atttacggca ggcatctatc
      901 cattcaaacg tgaagaagag gatcctacgc gtatgtttgc cggagaaggc ggtcctgaac
      961 gcacgaacaa acgctttcac tatgtgagtc tgggcatgcc tgcaaagcga ctgagcaccg
     1021 catttgactc ggttacttta tatggcaatg atcctgaata tcgtccggac atttatggaa
     1081 agatcggtaa ctcgggtgtg agcatttgtt gtctggatga cgcgaagaaa ttatacagcg
     1141 gatttgatct ggctgacccc aagacatcgg tttctatgac cattaatggt ccggcgccta
     1201 tgttattagg atttttcatg aatgcggcta tagatcaaca atgtgaaaaa tatattcgcg
     1261 ctaatggact cgaagctgaa gtagaagcca agatcaaagc aatttatgca ggcaaagaac
     1321 agctacgtcc aaaatacaat gccgctgaat taccacaagg aaatgacggt ttaggtttaa
     1381 tgcttttagg agttaccggc gatcaggttt tgcctgcaga tgtatatgct gatatcaaag
     1441 caaaaacact gagcgttgtt cgcggtacgg tgcaggctga tattttaaaa gaagatcagg
     1501 cacaaaatac ttgtattttc tctacagaat ttgcactacg ccttatgggt gatgtgcagc
     1561 agtttttcat cagcaataaa gtgcgcaatt tttattcggt atctatttcc ggatatcaca
     1621 ttgccgaagc cggtgcaaat cctatcacgc aattggcatt taccttggcg aatggattta
     1681 cgtatgtaga gtattatctg agtcgcggca tgcatatcaa tgattttggt ccgaatttgt
     1741 cgttcttctt cagcaatggc gtggatccgg aatatgcagt gattggtcgc gttgcacgac
     1801 gcatttgggc taaagcgatg aagcataaat acggtgctga tgatcgcacg caaaaattaa
     1861 aatatcatat ccagacatca ggtcgcagct tgcatgcgca ggagatagat tttaatgaca
     1921 tccgcacaac gctgcaggca ttgtatgcta tttatgataa ttgtaattcg ttgcacacca
     1981 acgcatatga tgaagcgatc accacaccaa cagaagaaag tgtgcgcagg gcgatggcga
     2041 ttcaattaat tatcaataaa gaattaggtc tcgcgaaaaa tgaaaatccg attcagggtt
     2101 catttatcat tgaagaatta acagatcttg ttgaagaagc cgtattatca gaattcgaca
     2161 ggattacaga acgcggtgga gtgttgggcg ctatggagac gatgtatcag cgcggtaaaa
     2221 ttcaggaaga gagtttatat tatgaaacgt tgaaacatac cggtgaatat ccgatcattg
     2281 gtgtgaatac tttcctcaat tcaaagggat caccaactgt gataccgatg gaagttattc
     2341 gtgcaacaga agaagaaaaa gaagcacaga tttccacctt gcgcaattta caatccatgc
     2401 aagtagaaaa agcgcatcac caattgcgtg aaattcaatt agcggctatt gaaaatcgaa
     2461 atattttcga gacgctgatg gaggcatgta aagtatgttc acttggacaa attacgaagg
     2521 cgatgtttga ggtgggaggg cagtatcgac ggaatatgtg agaaaaggaa aaaggagaga
     2581 ggagagagga gagaggagag agtttgtttc acgcagagac gcagaggcgc agagaggtga
     2641 tacatcaatg taacgaagtt acattgagtt tgggctgtct cactacccac tactcactac
     2701 cagttccgag acaccaaacc ttgttattgt gacattcgat tacagcttac tttagaatgg
     2761 tatcgctttc ctaaggaatg attttctaat ttagagttca aataatgtat catgcgcaag
     2821 cttatcctcg ctttattttt gattcctaca tgtgtttttg ctcaacagaa tgaaacagta
     2881 tcagcatcga tagatcgtac aatacttgat tcattgcata tggtgttgcg cgcagaaata
     2941 ttaaatcttg aggctgccaa gcaaaaagca cttgcaatgg gtttgcccgt ttccggttca
     3001 ggtgaggatg gtgctttcat gagtattcgt agtttgcgca atgggcagat catgtattat
     3061 aatactacca atctgaattc cgcaaagacc gatggcagca ataaattgtg gtcgggtggt
     3121 ggacttggat tgagtttgaa tggttcggga attgtggcgc gcgtttggga tgcaggtgca
     3181 ataaaaacta cgcaccagga actcaatgga agagcatcaa tgggtgtttc gcaaacggca
     3241 acactcagcg atcatgctac ccatgtttcc ggaactattg ccgcattcgg tgttgatgct
     3301 tcggcaaaag gacatgccaa tcaggctgaa ataaaaggtt tcgactggga tttcgatacc
     3361 gacgaaatga ccactgaagc aggaaatgga atgctggtga gcaatcatag ttacagctat
     3421 attgcaggat ggtacttcaa tggtggtaca ggacaatggg aatggtatgg cgatggaact
     3481 gtgagcaccg ttgaagatta tattttcgga aattatctgg atgaaagtag aaatgtagat
     3541 ttaatcgtaa atgcaagtcc gtattattta cctgttattg cagccgcaaa tgatcgcgat
     3601 gataatgtgc cgacaggagc aacttataaa atgatatggg atggcaccat acatactact
     3661 gatggcacac agccaaatcc tgatggcacc tatgattgta ttccaggagg attgcaaaca
     3721 gcaaaaaata cactaactgt tggcgcagtg aatgatattg taaatggtta tctcgcaccc
     3781 ggcgatgttg tattaaccgg attcacttct tatggtcctt gcgatgatgg aaggataaaa
     3841 cctgatgttg ttgccaacgg cacaagttta cattcctgtg tatctacaag caccacttcg
     3901 tactccgatt tcagcggcac cagtatggcc gcaccttctg tgaccggaag tgttgtatta
     3961 ttacaacaac atcaccataa tatatttggc acctacatgt taaatgccac tgcaaaagct
     4021 gtgatcatcc acaccgtaga taaggcatct gccacttttg gtccaactta tagttatggc
     4081 tggggactga tgaataccga tggcgctgca ttattaatta gtgacgcgct aatacatcct
     4141 attcgtatac aagagcggaa tttgccaaat ggagggacat ataattttac cggtaacagc
     4201 aacggcagtg aaccattgaa aattaccatt gcttggaacg atcccgcagc atcagttccg
     4261 acacctgccc ttgatccggt aactaaaatg ctggtaaacg accttgatct gcgtgtaacg
     4321 cgtttgagtg ataatactgt tttttatcct tataaattaa atccggcatc tcccggcact
     4381 gcagctataa caggtgataa taataccgac aatgttgaat atgtatacct ggcgacacca
     4441 agtgcaggag attataaaat cacgattaat cacaagggaa cattatcagg aggagatcag
     4501 gattacacta tagttgtgag cggactgagt tgcattcctt acagtttaac agaaaatgtg
     4561 actatcactg cacctgcaac atacactctt ccgaatggaa gcatagtgag cacaagtggc
     4621 acatatacga gcaatttcac cacttcgctc ggatgtgatt caaccatcat taccaatttg
     4681 actgtgaaca cattcacttg tgcagtacca acaggattgt ttaccaattc cattacttca
     4741 aacagtgcaa gagtaaactg gactgctgtt cccggggcaa caaaatatca gatttattat
     4801 cggcctgtag gcggtgcaac ctggattaag aaaactgcta ccaccaattc aaagaaatta
     4861 accggattag ctgcaaacac cacctacgaa tacaaggtaa aaactcaatg cgacccctac
     4921 cctgccagtg ggttttcagc tttggtaaat ttcactaccc ttccattgcg cgaaggcgaa
     4981 ataacttccg agtcggagag ctcgttggtt atttacccta atcctgccga agaaaattgc
     5041 acggtatcag ctgagggaat tgccgatggt gtatatcaca tgtatatcat caatttacaa
     5101 ggccagaagg tagttgagca aaccatacta ttttcaggcg atgactttac tgttgatgtg
     5161 aagaacctcg catccggcat gtataatttg gtattaagca acaacaatac acatatctcc
     5221 gatatacttg taattgcaaa atgacctagt tttgtttcat ggcattgacg gatgaaacat
     5281 tattaaaact cgaaacagca ctatcaccac tcgatagcaa aaaatggcag cataccatgt
     5341 caagggtatt gcgcatgata cttgagttct tcttcgttgc actcatagtc agcatgttta
     5401 tcgggtttct atccatgatg tcgaatggcg aaatgggcga tgatatgttt tttcgtgtca
     5461 tcagaggatt tatgttgttt gttttggcga tggtactttt tggtattatc cgcaaaactg
     5521 cccgcgacag gtttttgagt atgcaaagtg ctatcgctca tcagccatgg acattgaatc
     5581 tcatcacaat tgtatttaca gtttgtgcat ttgcgatagg atactttgca ggcactgctt
     5641 ttttaggagg caccttttca ccaacattta tgattaaatg ggccggcgct atcggttcga
     5701 tatttttttt gttgggacca tatatgttgc tgaagcgtat tgaagataac ttcacacaac
     5761 aatataaatc cattttattt gccaattttt tacccggaat tgcagcgcaa gccaggtatg
     5821 tgatgaatga acatatcggc acccagacat ttacggaaag taaattattt ccttatcagg
     5881 aaatttcatc cttgaaaggc agtgacctca tcacacgctc acaagaacaa tttcaatgta
     5941 gttttcttga tgttatgcaa gtggaacgca cccaatctaa cggcaagtca gaaactaaat
     6001 attctacttt attcaaagga tatttattat cggcagcatt caacaaatct tttaccggtg
     6061 aaacattcgt ggcaccggat gcttcgcggg aattacttgg tgaagtgtat ggtgaatcat
     6121 taaatgaact ttttcatcgc cccggaacga gcattgcaag aatggaagat cctgattttg
     6181 aaaaagcatt ttctgtgtat actactgatc agcaggaagc acggtatatt ctctcaacga
     6241 aattaattca tcgcattatg gagctgaaaa cttattttgt tgatgacgtg tatttatcgt
     6301 ttgtcggcaa tcgcgttttt gttgccgtga aaagtgaaaa ggatattttt tcaccgccaa
     6361 cgataggaaa tacttacaac aaaacgctgg ctgaaaagca atatgccatg ttgaatgcat
     6421 tagtaactgt tcccgagtta tttgatctcg accgaagaat ttgggcaagt tgaaacccaa
     6481 acacagccca taattttcta ccgacaaaca gttaaaaatc cttccttccg ttcaattcga
     6541 tcttcattgg tctgctgaaa gattcttcca atgaaataga agtttctgtc ctgtcaatgc
     6601 cattgattgg ctgaatacca tcatgaagca cctttttcag atgattggta tccttacaaa
     6661 tgatcttgag aaacatgcta taggttccgg tcatgtagtg acaatcgaca atctcaggga
     6721 tcttttcgag ctccttcact acactgtcgt acatagaact tttatctaga taaaccccta
     6781 ggaaagcggt tataccataa cccagtttct gtggattcac catcaaatgg gagcttttga
     6841 tgagtcccat ggcatccatt ttcttcatcc tcacatggat ggttcccggc gaaaccccta
     6901 ttttctgggc aatttcggta aaaggagtca ttgcgtcctg catcaaaaga tggaggattt
     6961 gaatgtctac tttatcaatt tcgtaatttt ctgacatggc ttggctgttc gattgcttat
     7021 tatgcaaata taatggatgt atttttataa aaacccaatt ttgctaaatt attattattt
     7081 tatttgcaaa tatcaatcgc taatttttat ctttgttata accttttaag gttattggtt
     7141 aggcaacata cccctaaaga atgctgtacg atgaggagcg tgcagcattc acttttttag
     7201 gctgtcccta atttgctgct accttcgttt tctaaatcta cagttgggtg aattttacct
     7261 tctttctaaa agggcaatgc tttcaacgtg atacgtttgc ggaaattgat cgaatggttg
     7321 aagcgccgtc accttgtaat catcgataag caattgtatg tcgcgagcct gtgtaaccgg
     7381 attacacgac acatacacga ttctttcagg agccagtttc aatagctgaa ggcaaacatc
     7441 tttatgcatg cctgctcttg gcggatctgt gatgactaca ttagctttgc catgctccgc
     7501 aataaaatcg tcggtaaata ttttggccat atcgcctgct atgaaagtgc agttatcgat
     7561 attattcagc tttgcatttt ctttcgcatc ggcaatagcg gcttcaacaa gttctactcc
     7621 aactactttt gagcacttat tagcagcttg caaagcaatg cttcccaaac cggtatacaa
     7681 atcatatacc ttatcgtccg gtttcaaagc tgcatatgca atcgttttcc tgtaaagatt
     7741 ttttgcctgt tcggtatttg tctggaagaa tgcgcgcgga ccgagttgat atttgatgcc
     7801 gtcaatttcc tcaatcatgt gtggtttgcc ggcaaataat gttacatcga tgtcataaat
     7861 aacatcattg tgcttggtat tgatacaata gtacagcgat gtaatttcag gaaatgcgtt
     7921 gcggatatgt tccatcatcg gcagcagctt ttccggttca tcaagtgcta caacaagtat
     7981 caccattaaa tcgccggtgc ctgtatttct aatgatgagg ttgcgcatat atcctgtatg
     8041 attgcgcaaa tcgtaaaatg caaaaccatg catcaaggca taggaacgca cagccattct
     8101 gatctcattg gaaagcgatc cctggagata acagcgttcc atatcaaaca cattggcaaa
     8161 ctgacctttc acatgaaaac cacaacccgg ctttttttca gggggatttg acttatcata
     8221 atgaggatca aaaatataag cgccattggt gaaagtatat tccattttat tgcggtaata
     8281 ccgatcataa ggcgatgcga taattttctc tatttcagga aatccgggag agcgtttcaa
     8341 caagcgaaag gcatcatcaa ccaattgctg tttgaattgt aattgcgttt cgtattcaac
     8401 attttgccaa gtacaaccgc cgcaaatatc gaaatgcgta caaaagggct ctatgcgtaa
     8461 atcactcttt ttatgaatag ctgttacgta tccggtatca tatccattgc gcttgcgggc
     8521 tacttggata tctacaatat cgcccggaat taccccttcg gtgaatacca ccttcccttc
     8581 taccctaccg aatgccgaac cgctgttggc aacggattca attaatacat gttgatggat
     8641 tttctttttg ttacgggcca tgatgacaat gtttggttca cgcagagac
//
LOCUS       DAOQOU010000140         8632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4452_length_8632_cov_5.133263, whole genome shotgun sequence.
ACCESSION   DAOQOU010000140 DAOQOU010000000
VERSION     DAOQOU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4452_length_8632_cov_5.133263"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLM90_11235"
     CDS             complement(<1..234)
                     /locus_tag="PLM90_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ89995.1"
                     /translation="MTFTSEQYRSFKLLSPAGCIIALLGFFLTFTEINCNGTTLDTIT
                     GVELATGYRPDIDLTESNDEPETKQEKYDPNVFA"
     gene            complement(244..1560)
                     /locus_tag="PLM90_11240"
     CDS             complement(244..1560)
                     /locus_tag="PLM90_11240"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ89996.1"
                     /translation="MYYNQTLVKLFALVVTAIFASSCNRIVVRYAGESDLIQASLPAP
                     GKVCKESENYIIDTNHLNATPIRYVRVNFHIMQESDGSGSWNEAEGRKFVHDIVEQSN
                     NRWNDNQPMTLPLGNSTPDLPIPIRIVLQRDPITGEEGIYFHRDDTLCYWNRSATKGP
                     NSLSDRTLISKYQVGIDSIINIFLIEHDPDSIGSPTYGEPKMSGISFINSIKLFSMYY
                     QGHIVKYTNAGVPFTHDAFYFSKLLNHELGHCFNLNHTWNWDDSCDDTPKNSGCWSVT
                     GVPPCDTPNSNNMMDYNISQQAVTPCQIGRMEYNFTKETGGARQFAIPYWCEYHAFER
                     VVVYRNENLEWNGAKDLWGDIEIRDGATLTIRCTVSLPEQAKVIIKPGGTLIIDGGTL
                     TNRCGDKFEGVEIWENKKTDQIGKVMISNGGKLENMVNYTTITTTE"
     gene            1587..2150
                     /locus_tag="PLM90_11245"
     CDS             1587..2150
                     /locus_tag="PLM90_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ89997.1"
                     /translation="MNRAYGLMLMIVACTFASCDQRGKEIKEIDAMAVKMDSVAKANP
                     VFTVAHKVEAEALITEYLEFVRQYPKDTLCAQYLMSAARLYEKMPAVKEELQLLDRLI
                     EEYPASPLVPQALITAANSCELVLRDYNRAIAYYRTLQEKYPDSPYSKNIDLQIEFIG
                     DPDGLFTAIMERAGKPVDTLESVNAVK"
     gene            complement(2130..2939)
                     /gene="cdaA"
                     /locus_tag="PLM90_11250"
     CDS             complement(2130..2939)
                     /gene="cdaA"
                     /locus_tag="PLM90_11250"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HMZ89998.1"
                     /translation="MQAAFKIGFLEITPVDVVDVLLVAFILFQLYRLVRGSLAINIFI
                     GLLMVYVLSLIAAALELTLMSDILGAFIDVGVIAVIIVFQPEIRRFLLYVGRSSDFRK
                     VRFWKALSLRKIRTGEYNQKEVNALTEAIMALSESKTGALIVIPQTSQLGFYQNTGIH
                     LNALITKDLIESIFYKNSPLHDGAMIISEHRIAAVKCILPLSDNPDLPGKLGMRHRSG
                     VGITEQSDATCIIISEENGEISYAREGKLYQQMQKEEIRKLLSRALFHSVN"
     gene            complement(2992..4674)
                     /locus_tag="PLM90_11255"
     CDS             complement(2992..4674)
                     /locus_tag="PLM90_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMZ89999.1"
                     /translation="MSQAQIPSLYLENKTPTYDETIAYFKHIDSMCDYMQMRSYGTTD
                     AGFPLDVLVIDGDKQFTPEAARAAGKAILLINNGIHPGEPDGIDACIQLITQYAGAGD
                     LKSALEGLVICIIPVYNIDGALNRNSTTRVNQEGPESYGFRGNGRNYDLNRDFVKCDT
                     RNSKSFAEIFHLWDPDIFVDTHVSNGADYQYVMTLIPTQHNKLTPPLGALLKNDMLPS
                     LYKAMEGKGFPMCPYVNEVDRIPDNGIAGFLDHGRYSTGFTALFNTIGFMPETHMLKP
                     YPQRVASTLAFIEVVIDYIQENRSAIIEARAVAKQTVIQADTFNLQWKLDTEQQESFM
                     FNGYAAKFKKSDVSGLDRLWYDRNEPFTKPIPFFDTYTPTLSIVAPEAYIVPQAWLPV
                     IERLQWNGVRMERLEKDTAISVESYYITGFDSVDRPWEGHYFHSNVKVRKVTETLNFM
                     KGDYIIPLNQESNLYIVQVLEPQAPDAFFAWGMMDAVLQQKEWFSDYVFEDLAAEILQ
                     QDPQLRNMLNEAMIADPSLKNDAEGQLAFVFEHSKYKEPTHNRYPIARIVKK"
     gene            complement(4806..5222)
                     /locus_tag="PLM90_11260"
     CDS             complement(4806..5222)
                     /locus_tag="PLM90_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90000.1"
                     /translation="MKSTKIAIIALLAYVGFGFTTIPGGEQQVKVSVGWEFKNLVEGF
                     DHDCKTELYIDGTLVATSSVTKESKANSVSATTSRGKHEIKVINYAYYEGNWEEHTVA
                     NNYSIDATYTDNINCKKKNTKIHLIFDIDKGTSVKK"
     gene            complement(5286..5918)
                     /locus_tag="PLM90_11265"
     CDS             complement(5286..5918)
                     /locus_tag="PLM90_11265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ90001.1"
                     /translation="MAHQIITVEGNIGSGKTSFATKLAKRFEAKLVLETFADNPFLQK
                     FFIKQQDYALGMEMFFLAERYEQLNNEMMDAELFQNRYVIDYLFNKSLLYARVNLDDA
                     EFALFQKIFNILNPKLRKSDVIVYLHSDLDRLVANIYKRGREFELTVKKDYLKKIETI
                     YLEYFRQAPDQKVLILDVTKADFVHNEADFDTMYDIVMQEHAIGVQRFEI"
     gene            complement(5983..6489)
                     /gene="folK"
                     /locus_tag="PLM90_11270"
     CDS             complement(5983..6489)
                     /gene="folK"
                     /locus_tag="PLM90_11270"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HMZ90002.1"
                     /translation="MMTEHTAYLLLGTNLGNRSENLSKATAAIRMFLGRVESQSHIYE
                     TEPWGKPHQPNFYNQAIKVVTPCSPLETLHLMKQVEFLLGRDNSEKWAPRVIDIDILF
                     FDELAIESPVLTVPHAHIAQRRFTLDPMSEIAPDLVHPVLNKTIKELADACTDTLAVQ
                     MVDVVYQF"
     gene            6586..8361
                     /gene="sppA"
                     /locus_tag="PLM90_11275"
     CDS             6586..8361
                     /gene="sppA"
                     /locus_tag="PLM90_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMZ90003.1"
                     /translation="MKPFFNSFFASLLALLVFTILGFFIIAGIAGAISQSFEKEKTSV
                     EGNSVLKLNLTYSIVEQTQSGMPGGLALLGLEADRGLGLNDILASIKHAETDENIKGI
                     YVQMGISPNGYATLQEIRDALADFKMESGKFVIAYGEVIGQNAYYLGSVADKVYMNPA
                     GAIDFKGLSAQLTFYKGALDKLGVTTQVFYDGKFKSATEPYRMDKMSPENRLQLEVFL
                     NGLFDANVSEIAADRKMQPMQCREIADSLLAWYPTEAVRLGLLDGLKYYDEVLDEMKS
                     RMGIANDQELKIVAMSDYRNSFTIPKVSDSKGTVAIVYADGTIIDGKAGNGFLGSADF
                     VEMMQQVREDENVKSVVIRVNSPGGSAVASDVMWREIEITKKKKPVVVSMGDYAASGG
                     YMISCNANKIYAQPNTLTGSIGVFLIVPEISDLMEDKLGITFDTANTGMYSDFPSITR
                     TFMPREQQILQSGVDSTYLHFKQMVAAGRNLSVEQVEEIAQGRIWIGEEAKKIGLVDE
                     IGGIDEAIAAAAKLGEIETYDIKEFPIQKESILDQVLVNLTEETKMQMQAEELGFMYP
                     HYRNLKELLERPVMQARMPYILEIK"
BASE COUNT         2438 a         1735 c         1892 g         2567 t
ORIGIN      
        1 tgcaaacaca ttcggatcgt atttttcctg ctttgtttca ggttcatcgt tagactctgt
       61 cagatcgata tccggtctgt aacctgtagc taattcaaca cctgtaatgg tatctaaagt
      121 ggtgccattg cagttgattt cagtaaacgt aagaaagaaa cctagtaatg caataatgca
      181 tcctgcagga gacaataatt taaatgatcg atattgttct gaagtaaacg tcatattgct
      241 tgtttattct gttgtagtga tagtcgtata attgaccatg ttttccaatt tcccgccatt
      301 actgatcata acttttccga tctgatccgt ttttttattt tcccagattt caacaccttc
      361 aaatttatca ccgcagcgat ttgttaatgt gccgccgtca atgatcaatg tgcctcccgg
      421 tttaataatt actttagcct gctcaggaag tgaaaccgtg catcgaatag tgagtgttgc
      481 gccgtcacga atttctatat cgccccatag atcttttgca ccattccatt caagattttc
      541 attgcgataa actaccacgc gttcaaatgc atgatactca caccaataag gaatggcaaa
      601 ttgtcttgcg ccaccggttt cttttgtaaa attatattcc atgcgaccaa tctgacatgg
      661 agtaacagct tgctgactga tattgtaatc catcatatta tttgaattag gggtatcgca
      721 agggggtact cctgtaacag accagcagcc tgaatttttt ggggtgtcat cacaactatc
      781 atcccaattc catgtatgat ttaaattaaa gcaatgtcct aattcgtggt tcaacaattt
      841 agaaaaataa aaggcatcat gcgtaaaggg cacaccggca ttggtgtatt tcacgatgtg
      901 accctgataa tacatactga acaatttgat gctgttaata aaacttatcc ctgacatttt
      961 gggctctccg taagtcggac tgccaatact atcgggatcg tgttcaatca gaaatatatt
     1021 aataattgaa tctatgccta cttgatattt gctgattaat gttctgtcgc ttaatgaatt
     1081 gggacctttt gttgcactgc gattccaata acataaagta tcatcgcggt gaaaataaat
     1141 accttcttca cctgtaatgg gatcgcgttg caagacaatg cgaatgggga ttggtaaatc
     1201 aggcgtgctg ttacccagag ggagtgtcat tggctgatta tcgttccatc ggttattgct
     1261 ttgctcaaca atgtcgtgaa caaattttct accttcagcc tcattccaac ttccactgcc
     1321 atcactttcc tgcatgatat gaaaattgac gcgcacatag cgaatgggtg ttgcgttgag
     1381 gtgattggta tcgatgatat aattttcgga ttccttgcaa acttttccgg gtgcaggtaa
     1441 agaggcttgt ataaggtcac tttcgccggc atatctaact acgatccgat tgcatgatga
     1501 cgcaaaaatg gcggtcacca ccagggcaaa aagcttgact aaggtttgat tgtagtacat
     1561 ttgtggctct aaaataaaga tatatcatga atagagctta tggcttaatg ttaatgattg
     1621 ttgcgtgcac attcgcttct tgtgatcagc ggggtaaaga aatcaaggag atagatgcaa
     1681 tggctgtaaa aatggatagc gtggctaagg ctaatcctgt ttttacagtt gcacataaag
     1741 tagaagctga agctctgatt accgaatacc ttgaatttgt tcgccaatat cctaaagaca
     1801 cgctttgtgc tcaatacctc atgagtgcag cgcgtttata tgaaaaaatg cctgctgtaa
     1861 aggaagaatt gcaattactg gataggttga ttgaagaata tcccgcatca ccattagtgc
     1921 cacaagcact gattacggca gcaaatagtt gtgagttggt gctgcgcgat tacaatcgtg
     1981 caattgccta ttatcgcacg ttgcaagaga aatatcctga tagtccttac tcgaaaaata
     2041 tcgaccttca aattgaattt atcggcgacc ctgatggatt atttactgct attatggaac
     2101 gcgcaggaaa accggttgat acgcttgaat cagttaacgc tgtgaaataa agccctgctc
     2161 agcaatttgc gaatctcttc cttttgcatt tgctgataca attttccttc tcgcgcataa
     2221 cttatttcgc cattctcttc actaataata atgcaggtag catcactctg ttcggtaatg
     2281 cctacaccac ttcgatgacg catgcctagt ttacccggaa gatccggatt gtctgataat
     2341 ggcaagatac atttcacagc tgctatccgg tgttcagaaa tgatcatcgc accatcgtgt
     2401 aatgggctgt ttttatagaa aatactttct atgagatcct ttgttatcaa ggcattcaga
     2461 tgtatgcccg tgttttgata aaatcccagt tgcgatgttt gaggaattac tattaatgcg
     2521 cctgtctttg attcagacaa tgccataata gcctctgtta atgcatttac ttccttctga
     2581 ttatactctc cggttcgaat ttttcgaaga ctcaaagcct tccaaaagcg cacctttctg
     2641 aaatcactgc ttcttcctac atacaataaa aagcgtcgga tctcaggctg gaaaacaata
     2701 ataacggcaa ttactccgac atcgataaat gcacccagaa tatcgctcat taatgtgagc
     2761 tctaatgctg cggcaatcaa actcaacaca taaaccatca ataagccgat aaatatgttg
     2821 atggcgagac tgcctcttac aagtcggtat aactgaaata aaatgaatgc aaccagcaac
     2881 acatcaacca catccaccgg ggtgatttcc aaaaatccta ttttgaatgc tgcctgcatg
     2941 cggtaaaact acttaatttc gaatgcctga tctttgttca tgaacccttt actatttttt
     3001 tacgatgcgg gctatcggat aacgattatg tgtcggctct ttgtatttac tgtgttcgaa
     3061 tacaaaagca agttggcctt cggcatcgtt cttcaatgaa gggtctgcaa tcattgcttc
     3121 attcagcatg tttctcagtt gcggatcctg ttgcaatatt tccgctgcca ggtcttcaaa
     3181 cacatagtcg ctaaaccatt ctttttgttg caacacggca tccatcatcc cccaggcaaa
     3241 aaaagcatcc ggagcctgag gttcaagcac ctgcacaatg tagaggttcg actcctgatt
     3301 cagcggaata atgtaatcgc ccttcataaa gttcagcgtt tccgtaacct tgcgaacctt
     3361 aacattgctg tgaaaataat gcccttccca aggcctgtca accgagtcaa aaccggtgat
     3421 gtaataagat tcaactgaaa tggcggtatc cttttccagt cgctccatcc tgacaccatt
     3481 ccattgcaat cgctctatca ccggcaacca tgcctggggc actatataag cctcaggagc
     3541 aacgattgat agggtaggcg tataggtgtc gaaaaagggt atcggcttcg tgaagggctc
     3601 attgcggtcg taccagagcc tgtcgagacc tgaaacatca cttttcttga acttggcggc
     3661 atacccgttg aacataaacg attcttgctg ctcagtatcc aatttccatt gtaaattgaa
     3721 cgtatctgcc tgaatgacag tctgttttgc aacagcccga gcctcaatta ttgcgctacg
     3781 gttctcttgt atgtaatcga taaccacttc tataaatgcc aacgttgagg ccactcgctg
     3841 cgggtatggt ttcaacatgt gcgtttccgg cataaagcct atggtattga aaagcgccgt
     3901 aaacccggtt gaataccgac cgtggtccag aaatccggca ataccgttat cagggatgcg
     3961 gtctacttcg ttcacataag ggcacatggg gaaccctttt ccttccatcg ccttgtataa
     4021 ggaaggaagc atatcgttct tcagtaaggc tcccaagggt ggtgtcaatt tgttatgttg
     4081 agtgggaatt aatgtcataa catattgata atcagcacca ttgctcacgt gggtgtctac
     4141 aaaaatatcc ggatcccaca ggtgaaagat ttctgcaaac gatttgctgt tacgagtatc
     4201 acatttcacg aaatcgcggt tgaggtcgta gtttctgcca ttgcctctga agccataaga
     4261 ctcaggtccc tcctggttta cgcgtgtagt gctgttccga tttaaggcac catcgatatt
     4321 gtaaaccgga ataatgcaaa taaccagtcc ttcgagcgct gatttcaaat cgcctgcacc
     4381 tgcatattgc gttatcaatt gtatacaagc gtcgatccca tccggttcgc cgggatgtat
     4441 gccattgttg atcaggagta ttgcttttcc tgctgctctg gctgcctcag gtgtaaattg
     4501 tttatcgccg tcgatcacca aaacatcaag cggaaatcct gcatcagtgg tgccatagga
     4561 ccgcatctgc atgtaatcgc acatgctgtc tatatgcttg aaataggcaa tggtttcatc
     4621 ataggtaggg gtcttgtttt cgaggtatag tgaagggatt tgtgcttgtg acatagtagt
     4681 tagagtaagt atggataaaa cggaaataat agttcgcata gcctggagtg cctgatggaa
     4741 gggataaaaa taaaaaaacc tttcagttgt gccgaaaggt ttcattgtgt gcattttgaa
     4801 tggaattatt ttttcaccga agtgccctta tcaatatcaa aaatgagatg gatcttggtg
     4861 tttttctttt tacaattgat attatctgta taggtagcgt cgatactata attgttggct
     4921 acagtatgct cttcccagtt gccttcataa taggcgtaat tgatcacttt gatttcgtgt
     4981 tttccgcggc tagttgtagc agagactgaa ttggctttgc tttcctttgt tacgcttgaa
     5041 gttgcaacca gtgtgccatc gatatacaat tcagttttgc aatcgtggtc gaatccttca
     5101 accaggtttt tgaattccca tccaacagaa acttttactt gctgttcacc accgggaatt
     5161 gtggtgaatc cgaatccgac ataggccagg agggcaataa ttgcaatttt agtgcttttc
     5221 ataacttgaa tttgtgtcac aaatatatat atttctgaca tatatctgac aatcactaga
     5281 ctatattaga tttcaaatcg ttgcacgcca attgcatgtt cttgcattac gatgtcgtac
     5341 atcgtatcaa aatcggcttc attgtgcacg aaatcggcct tggtgacgtc taaaatcaat
     5401 actttctggt cgggtgcctg gcggaagtat tcgaggtaga tcgtttcaat tttcttgaga
     5461 tagtctttct ttaccgttag ctcaaattcg cggccgcgtt tataaatatt ggcaacaagc
     5521 ctgtcgaggt cagagtgcag gtaaacaatc acatcgcttt tgcgcagttt ggggttgagg
     5581 atattgaaga tcttctgaaa cagcgcaaat tcggcatcat caaggttaac acgggcgtag
     5641 agcagtgatt tattgaacag atagtctatt acataacgat tttgaaacag ctcggcatcc
     5701 atcatttcat tattgagctg ttcatacctt tcagcaagga agaacatttc catgcctaag
     5761 gcatagtctt gttgtttgat aaagaatttt tgcaggaaag ggttgtcggc aaaggtttcg
     5821 agtaccagct tcgcttcgaa gcgtttagcc agtttggtgg caaacgatgt tttgccggaa
     5881 ccgatattac cttctactgt aatgatttga tgcgccatac ctgaaagggc gtcaaaaata
     5941 caggaaaacg aagaataaaa ttgaaaatgt tgagttcacc tgtcagaact gataaacgac
     6001 atcaaccatt tgcacggcga gggtgtctgt gcaggcatct gcaagctctt tgatggtttt
     6061 atttaacaca ggatgcacca aatcgggagc aatctcactc attggatcga gtgtgaacct
     6121 acgctgggca atgtgagcat gtggaactgt aagcacaggt gattcgatgg ctagttcatc
     6181 aaaaaacagg atatcaatat caataaccct tggagcccat ttctccgaat tatcgcgtcc
     6241 cagcaggaac tcaacctgtt tcatgaggtg aagcgtttca agcggtgagc aaggtgttac
     6301 aactttgatt gcctgattgt agaaattggg ttgatgtggc tttccccaag gctcagtctc
     6361 atagatatgc gactggcttt ctacacgtcc aaggaacata cgaatggctg ccgtggcctt
     6421 agagagattc tccgaccggt tacctaaatt tgtccctaac aataaatagg cggtgtgttc
     6481 cgtcatcatt ggctgtcgag gagttatttc ctttagggga ataacgtaat cctgcgaaaa
     6541 ttagtgcatt catcaataag taggtaataa agagtaagaa aatttatgaa accgtttttt
     6601 aattcctttt tcgccagtct tctggccctt ctggtattta ccatattagg attttttata
     6661 atagctggca ttgccggtgc tatctcacag agctttgaga aggaaaagac ttctgtggag
     6721 ggaaattctg ttttaaaact gaatttaaca tattccattg tagaacaaac gcaaagtgga
     6781 atgccgggcg gattggcatt gctcggactt gaagctgatc gcgggttggg attgaatgat
     6841 attctggctt ccatcaagca cgctgagact gatgaaaaca tcaaagggat ttatgtgcaa
     6901 atgggtatta gtccaaatgg atatgccaca ttgcaggaaa ttcgtgatgc gctggcagat
     6961 tttaaaatgg agagtgggaa attcgtcatt gcttacggtg aggtaatagg gcagaatgct
     7021 tactatcttg gaagcgttgc cgataaagtt tatatgaatc cggcaggagc tatagatttt
     7081 aaaggattga gtgcgcaact cacattttat aaaggagcgt tggataaatt aggtgtgact
     7141 acgcaagtat tttatgatgg caaatttaag agtgccactg agccttatcg catggataaa
     7201 atgagtccgg aaaatcgctt gcaactggaa gtgtttttga acggactttt cgatgccaat
     7261 gtgagtgaga tcgccgcaga tagaaaaatg caaccgatgc aatgcagaga aattgccgac
     7321 agccttttag catggtatcc aaccgaggct gtgcgattag gtttgctcga tggactgaaa
     7381 tattatgatg aagtgttgga tgaaatgaaa tcgcgcatgg gcattgccaa tgatcaagaa
     7441 ttgaaaattg tagcaatgag cgattatcgc aattcattta ctattccaaa agtatctgat
     7501 agcaagggaa ctgttgctat tgtttatgct gatggtacca tcatagatgg taaggcagga
     7561 aatggatttt taggttctgc tgattttgtg gaaatgatgc aacaagtgcg agaggatgaa
     7621 aacgtaaaat ctgtggtgat ccgcgttaat tcacccggcg gttctgccgt tgcgagtgat
     7681 gtgatgtggc gtgaaattga aatcaccaaa aaaaagaaac ctgttgttgt ttctatgggc
     7741 gattatgctg ccagtggcgg gtacatgatt tcatgcaatg ccaacaaaat ttatgctcaa
     7801 ccaaatacct tgaccggaag cattggggta ttcctaattg tacctgaaat ttcggattta
     7861 atggaagata aacttggtat cacatttgat acggcaaata caggtatgta ttcagatttc
     7921 ccttctatca ccagaacatt tatgccgcgt gagcaacaaa ttttacagtc gggtgtagat
     7981 agtacttatc tgcattttaa acaaatggtt gcagcaggaa gaaatttgtc ggtagaacag
     8041 gtggaagaaa ttgcacaagg gagaatatgg ataggagagg aggctaaaaa aatcggattg
     8101 gtggatgaga tcggcggtat cgacgaagcc attgcagcag cagctaaatt gggtgaaatt
     8161 gaaacttacg atatcaaaga atttccgatt caaaaagaga gcattctcga tcaagtactg
     8221 gtaaacctga cggaagaaac aaaaatgcaa atgcaggcag aagaattagg gtttatgtat
     8281 ccgcattatc gcaatctcaa agaattatta gaacgccccg tgatgcaagc aagaatgcct
     8341 tatattctgg agattaaata agtaatcaaa aaaattaatt cacctcacaa aatcggctcc
     8401 gaatttcaat gcttcggagc cgattttatt ttcagcaatc gttgtgagta ggtgagtggg
     8461 tgagtgggtc agtgggtgag tggtgaatcg tgactcgtta ctcgtgaatc gtgagtgcca
     8521 actttgcgac tttgcaactt tgcgactttg cgcgaacccc acctcacccc ctgaccccct
     8581 ctccactcct ggcgtcgcag agagggggaa ttaaatgcat ttgatgatga at
//
LOCUS       DAOQOU010000141         8575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4498_length_8575_cov_6.408803, whole genome shotgun sequence.
ACCESSION   DAOQOU010000141 DAOQOU010000000
VERSION     DAOQOU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4498_length_8575_cov_6.408803"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLM90_11280"
     CDS             complement(<1..516)
                     /locus_tag="PLM90_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMZ90004.1"
                     /translation="MAEKKEKRSKLFSGEDYKIFRRLMSYAGPFRRQFIISTLLAIGL
                     SILTPLRPYLVGITVDDYIMTHDFHGLLILTIISLVVLFAESIGRYFFLYTTNWLGQH
                     VVKNLRVKVFEHLVGLRLSYFNRTPVGISTTRTINDLETINSVFTEGAIQIIADLLTI
                     IFVITFMFITSW"
     gene            complement(553..1743)
                     /locus_tag="PLM90_11285"
     CDS             complement(553..1743)
                     /locus_tag="PLM90_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HMZ90005.1"
                     /translation="MQQLQEQIKNMASEYFEDIRTCRRYIHRHPELSFFEKETAAFIQ
                     QKLTEYHIPFEANIAGNGIVAIIAGKNPSKKTIALRADIDALPITEANDVVYASSNKG
                     VMHACGHDAHTASLLGAGRILNNLRDAFEGTVKLIFQPAEEKLPGGAKLMIEAGVLNN
                     PNVNAIIAQHVFTPFKVGTVAYCFGTAMASTDELYITIHGKGGHGAYPQDTIDPVMIS
                     AQLLVALQQVVSRTVSPFQPAVLSFGKVIADGATNVIPDKAYLEGTFRALDETVRADA
                     HKKIVEIATHIAEAFGARAEVRIEKGYPVLHNDEALTRRSYDRAVAYLGQENVFITTP
                     RMGAEDFAYYSREIPACFYRLGTGNPEKGIVSNIHTPTFDIDEDALKIGMGLMAWHAI
                     GELL"
     gene            complement(1745..2131)
                     /locus_tag="PLM90_11290"
     CDS             complement(1745..2131)
                     /locus_tag="PLM90_11290"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ90006.1"
                     /translation="MKLRSLITILAILMCAMAKAQSGNIVINDEAGAAELIEKHIMFN
                     KDHGVLPGYRIQIYSGTSLTTAKEEKSDFLQVFDVPAVIVFEAPNYKVRVGNYLNRFE
                     ANTDLQTLNLDYPDALIVKDLITVNQ"
     gene            complement(2165..2833)
                     /gene="deoC"
                     /locus_tag="PLM90_11295"
     CDS             complement(2165..2833)
                     /gene="deoC"
                     /locus_tag="PLM90_11295"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HMZ90007.1"
                     /translation="MSTPKNLSAYIEHTILKPDTTHAAVEQVCHEALQHHFAGVCVPS
                     YFLEQAADLLQDSDAELITVVGFPYGYNSTMSKFEEMEDAMHNGALHIDVVVNIAAIK
                     NNDWDHVETELSTLTRLAHSEDRIIKVIAESGMMSEVELDLIIRIANEVGIDYLKTST
                     GVNGTGATAEAIEYMRKHLNPAIRIKASGGIRTREQALTFIGLGADRIGASASVAIVT
                     EGNN"
     gene            complement(2844..6170)
                     /gene="secA"
                     /locus_tag="PLM90_11300"
     CDS             complement(2844..6170)
                     /gene="secA"
                     /locus_tag="PLM90_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMZ90008.1"
                     /translation="MSVLTNMISKLFGGNKYEKDIKSIMPLVDDINREFAKLSTLSND
                     QLRAKTDEFKGRIKAHLSEIDNRISSLKIQSDDPTLEDVDQKDEIYKQIDALRKDRDK
                     LIEEILLKLLPEAFAVMKETARRFTENTEIISTATELDKKISVAHDFVVIDGDKAIYK
                     NSWEPAGVPITWNMVHYDVQLIGGIVLHQGKISEMATGEGKTLVATLPAYLNALAGEG
                     VHIVTVNNYLARRDAEWNGPIFNFLGMRVDCIDLYQPNSPERRNAYLADITYGTNNEF
                     GFDYLRDNMVRSIEEKVQRKHHFAMVDEVDSVLIDDARTPLIISGPVPKGGDAEVYNV
                     LKPRINKLIDAQKRIFNTSLNEAKKLIEAGKAKEGGIHLLRAHRALPKNNALIKFLSE
                     EGIRRLLLETEGVYIAEHNKRMPEIDQELFFVIEEKNNSVDLTDKGIELITEGSEDPQ
                     FFIIPDVGIQIAEIERSLRPKEEQIEMKDNLMRDFTIKSERIHSVQQLLKAYTLFEKD
                     VEYVVMDGKVKIVDEQTGRILEGRRYSDGLHQAIEAKEAVKVEALTQTYATITLQNYF
                     RMFHKLCGMTGTAETEAGEFWEIYKLDVTAIPTNRSIVRNDMEDYVYKTRKEKYLAVI
                     DRIQALVKDGRPVLVGTTSVEVSELLSRMLQLKGVKHNVLNAKQHQREAEIVTDAGKA
                     GSVTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSS
                     EFYVSLEDNLMRLFGSERIAKVMDTMGYKEGEVIQHRMITKSIERAQKKVEENNFGIR
                     KRLLEYDDVMNNQRTLVYQKRNHALSGERLTIDLNNTIYDLCEELVSTHVESKDYEAF
                     TMDVIRYFSMEPEVSKHDFENEKPLVVTQKLYHSAREFYRRKSQQVVNKVLPLINDLY
                     TTRGETLENVIVPFSDGRKGINVMVNLKRAVDTQGVEILYSIERMISLIVIDDNWKDH
                     LRNMDDLKQSVQGAVYEQKDPLLIYKFEAFEMFKKMLSELNREIVSFLFKGSVPLRTP
                     DEVRTAAPQKTQTDMSQLKTGREEAGAGPGARPNAPQAQQERRAIEPVRVEKKVGRND
                     PCPCGSGKKYKNCHGKDEVN"
     gene            6348..>8575
                     /locus_tag="PLM90_11305"
     CDS             6348..>8575
                     /locus_tag="PLM90_11305"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HMZ90009.1"
                     /translation="MPATIQQISALLGQQGQIVSPQAPVIQVLTDSRTIFSGADSLFF
                     AISGAQHNGLQYVEQAYAAGVRSFVVEKLPSTLHGDANYLLVENAIDALQIIAAAHRL
                     QFQFPVIGITGSNGKTIVKEWLYHLLKQQLDIVRSPKSYNSQIGVPLSVWKMDERNEL
                     GIFEAGISQPGEMQKLQQVIRPTHGIITNISDPHREGFENDAQKLSEKLQLFKEASSL
                     VYCADHEQISDAIKIILPNTKTIAWSARSNNADVIISDIEQLPDSTMFTLKYDEHNLH
                     FKIPYSDSASLENSIHAIVMSLVIMNERKTLGAFTISNITIQAATLPVVSMRLELKRA
                     DHNSLLINDAYSADIASLEIALKFQAQHSTGLQRVVILSDFDQSGLERQLLVGEILKL
                     LSAYQVDRIIGIGEGWHANNEIDKSRVQTFADTETCIAYLQQQPISNAVILIKGARRF
                     TLERIGQLLELKTHGTALRIHLDHMVSNLQVYRSILKPGVKTMVMVKAFSYGSGQAEI
                     ARLLAHQRVDYLAVAYADEGVDLRRQGIRLPIMVMNPEPETFDQLLDAQLEPEIYSMR
                     ILNLWLQAVQNRKAHSENKQYVVHIKLDTGMHRLGFEASDIDVLIDTLQQHPHIHVAG
                     IFTHLAATDSADHHAFTKLQLQKFESMSTRITDALGYAVIRHALNSSGISNYPEAQFD
                     MVRLGIGLYGVDPSSTVQSRLKHVSTLVTNIAQIRNIQEGESVGYSCSFIAPKPMRIA
                     TI"
BASE COUNT         2387 a         1886 c         1761 g         2541 t
ORIGIN      
        1 ccaactggtg atgaacatga aggtaatcac aaatatgatc gtcagcagat cggcaatgat
       61 ttgaatggcg ccttccgtaa atacagaatt gatggtttcc aaatcgttaa tggtgcgcgt
      121 ggtggaaatg ccaacgggtg ttctgttgaa gtaactgagt cgcaatccaa ccaaatgttc
      181 gaatactttt acgcgcagat tttttacgac atgctgacct agccagttag tagtgtataa
      241 aaagaaatat ctccctatgg attcagcaaa taaaaccaca agggaaatga tggtcaatat
      301 caacaatcca tgaaaatcgt gggtcatgat ataatcatca accgtaattc caactaaata
      361 aggtcgcagg ggagtgagta tggaaagacc gatggcgagt aaagtggaga tgataaattg
      421 tcggcgaaag ggaccggcat aagacatcaa ccggcgaaat atcttataat cttcaccgct
      481 gaataacttt gatcgctttt cttttttttc cgccacaata aattaaatgg tgcaggcaca
      541 acaacgtacc tgttacaaga gttcaccaat agcatgccat gccatcaatc ccatacctat
      601 tttcaatgcg tcttcatcga tatcgaaagt tggggtatgt atgtttgaaa cgataccttt
      661 ttccggattt cctgttccca accggtaaaa acaagccggt atttcccggg aataataggc
      721 aaaatcctca gcacccattc gaggtgtggt gatgaatacg ttttcttgtc cgaggtaagc
      781 cactgccctg tcataactgc gacgtgtcaa ggcctcatca ttatgtaaaa cgggataccc
      841 tttttctata cgcacttcgg cacgcgctcc aaatgcttca gcaatatggg tggcgatctc
      901 aactattttt ttgtgtgcgt cggctctaac tgtttcatct aatgcgcgaa aggtgccttc
      961 caaatatgct ttatcaggta tcacattggt agcgccatcg gcaatcactt taccgaaact
     1021 taatacggca ggctgaaaag gtgaaacagt tctgctcacc acttgctgca atgcaacgag
     1081 taattgcgca ctgatcatca cagggtcaat agtatcttga ggataggcac cgtgccctcc
     1141 tttaccatga atagtaatat aaagttcatc ggtgctcgcc atggcagtgc cgaaacagta
     1201 tgctacagtt ccaaccttaa atggtgtgaa tacatgttgt gctatgatgg cattgacatt
     1261 tggattattc agcacacccg cttctatcat caattttgca ccgccgggta atttttcttc
     1321 tgcgggttga aagattaatt tcaccgtacc ttcaaatgca tcgcgcaaat tattgagtat
     1381 tctgcccgcc cccaaaagac ttgctgtatg ggcatcgtgg ccgcatgcgt gcattacgcc
     1441 cttgtttgaa gatgcgtata ctacatcatt ggcttcggta atcggcaagg catcgatatc
     1501 ggcgcgcagt gcaatggttt tctttgatgg gtttttcccg gcaataatgg caacaatacc
     1561 attacctgca atattcgctt caaagggaat atgatattca gttaatttct gctgaataaa
     1621 tgctgccgtt tctttctcaa aaaatgaaag ttcaggatgc cgatgtatat aacggcgaca
     1681 agtgcgaatg tcttcaaaat attcgctcgc catatttttt atttgttcct gcaattgctg
     1741 catgttattg attgacggtg atcagatctt tcactatcaa tgcatcggga taatctaaat
     1801 tgagtgtttg cagatcggta tttgcttcaa atcgattgag ataatttcca acgcgtactt
     1861 tataattcgg cgcttcaaaa acgattaccg caggaacatc aaatacctga agaaaatcag
     1921 atttttcttc tttcgcggta gttaaacttg tgcctgaata aatttgaatg cgataacccg
     1981 gtaatacacc atgatcttta ttgaacatga tatgtttttc gatcaactcg gcagcaccgg
     2041 cttcatcgtt gatgacgata ttacctgact gcgccttagc cattgcgcac atcaaaatag
     2101 caagtatggt aatgagcgat cgaagtttca tatcagctaa ggtagtaatt cgggcgtaaa
     2161 ttgatcaatt attgccttct gtaactatcg ccacactagc cgaagcaccg atccgatcgg
     2221 cacccaggcc tataaaggta agcgcctgct cgcgtgtgcg aatgcctccg gaagctttta
     2281 tcctgatggc agggttcaga tgcttacgca tgtattcaat cgcctcggca gtggcacctg
     2341 ttccgttcac acctgttgaa gttttcaaat aatcaatacc tacttcattg gcaatacgaa
     2401 taataaggtc taattccacc tccgacatca tgcctgattc cgcaatcact tttataatgc
     2461 gatcttcact gtgtgcaagc ctggtgagtg tcgacaattc agtttctaca tgatcccaat
     2521 cgttgttttt tatagctgca atatttacaa ctacatcaat atgtaaggca ccattatgca
     2581 tagcatcttc catttcttca aacttgctca tggttgaatt ataaccataa ggaaaaccaa
     2641 ctaccgttat caattccgca tcactatcct gcaatagatc tgctgcctgc tctaaaaaat
     2701 aggaaggaac gcatacaccg gcaaaatgat gttgcaatgc ttcatgacaa acttgttcta
     2761 cggcagcatg cgtagtatca ggttttaaaa tggtatgctc aatatatgcg cttaaattct
     2821 taggtgtcga catgcaatct ggtttaattt acttcatcct taccgtgaca attcttgtat
     2881 ttcttaccgc ttccgcatgg acatggatcg ttgcgtccga cttttttctc cacgcgcacc
     2941 ggttcaatgg cgcggcgttc ttgttgagct tgcggagcat ttggtcttgc tcccggaccg
     3001 gcacctgctt cttcgcggcc ggttttcagc tgactcatat ccgtttgcgt tttttgcgga
     3061 gctgctgtgc gcacttcatc aggtgtgcgc agaggcactg aacctttgaa caagaaggaa
     3121 acgatctcgc ggtttaattc gctcaacatt ttcttgaaca tttcaaaggc ttcgaattta
     3181 tagatcaaca acggatcttt ctgctcgtaa actgctcctt gtaccgactg tttcaagtca
     3241 tccatattgc gcaaatgatc tttccagttg tcatcaatta cgatgaggct gatcatgcgt
     3301 tcaatagaat ataatatttc aacaccttgt gtatcaacag cgcgcttcag attcaccatc
     3361 acattaattc ccttcctgcc gtcgctgaaa ggcacgatca cgttttcgag tgtttcaccg
     3421 cgggtagtat ataaatcgtt gatcaaaggc aatactttgt ttactacctg ctgcgatttg
     3481 cggcgataaa attcacgtgc agagtgatat aatttttgtg ttacaacgag aggcttttca
     3541 ttttcaaaat catgcttact cacttcaggt tccatgctga aatagcggat cacatccatg
     3601 gtaaaggctt cgtaatcttt gctttcaaca tgggtgctca ctaattcttc acacagatcg
     3661 taaatagtat tatttaaatc tatggtcagg cgctcaccgg ataatgcgtg attacgtttt
     3721 tgatacacaa gggtacgctg attattcatt acatcgtcgt actccaacaa acgtttacgt
     3781 ataccgaagt tattttcctc cacttttttc tgtgcgcgtt ctatggattt cgtgatcatg
     3841 cgatgttgaa tcacttcacc ttctttgtaa cccatggtat ccatcacctt tgcaatacgt
     3901 tcgcttccga ataagcgcat gaggttgtct tcaagagaaa cataaaattc gctggaacca
     3961 ggatctccct gacgacctgc acgaccgcgc aactgacggt caacgcgacg agattcgtgg
     4021 cgttcagtac caatgatggc aagaccaccc gcttctttaa cgccggggcc gagcttgata
     4081 tccgtaccgc gacccgccat gttggttgcg atggttacgc ttcctgcttt acctgcatcg
     4141 gtaacgattt cagcttcacg ctggtgttgc ttcgcgttga ggacgttatg tttaacacct
     4201 ttcaactgca acatcctgct caataattca ctcacctcaa cactggtggt acccaccaat
     4261 accgggcgac cgtcttttac caatgcctga atgcgatcga taacagcgag atatttttct
     4321 ttacgtgttt tatatacata atcctccata tcgttcctga caatggaacg gttggtagga
     4381 attgcggtaa catccaattt ataaatttcc cagaactcac cggcttctgt ttctgcagta
     4441 cctgtcatgc cacacaattt gtggaacatg cggaaataat tttgcagtgt gatggttgca
     4501 tacgtttgag taagtgcttc cactttcaca gcctcttttg cttcaatagc ctgatgcaat
     4561 ccatcgctgt aacggcgacc ttcgaggata cgaccggttt gttcatcaac aattttcact
     4621 ttgccatcca tcaccacata ttccacatct ttttcaaaca atgtatatgc tttcagtaat
     4681 tgttgcacag agtggatgcg ttcactttta atggtgaaat cgcgcatcag gttatccttc
     4741 atttcaatct gctcctcttt aggacgcagt gatcgttcaa tttcagctat ttgaataccg
     4801 acatcaggaa tgataaagaa ttgcggatct tcacttcctt cggtaattag ctcgatacct
     4861 ttatcggtga ggtcgacact attatttttt tcttcgatca caaaaaacaa ttcctgatca
     4921 atttcaggca tgcgtttatt atgctcagcg atatagacac cttctgtttc taacaataac
     4981 cggcgaatac cttcctcact taaaaatttg atgagtgcat tattttttgg aagcgcacga
     5041 tgggcacgca ataaatgaat gccgccttct tttgcctttc ctgcttcaat taatttttta
     5101 gcttcattta agctggtatt aaaaatgcgt ttttgtgcat caatcaattt attgatacgc
     5161 ggtttcaata cattatatac ttcagcatca ccacccttag gtacgggacc actgatgatc
     5221 aaaggtgtac gggcatcatc gatcaataca gagtcaacct catccaccat agcgaaatga
     5281 tgtttgcgtt gcaccttttc ttcaattgag cgcaccatgt tatcacgcag gtaatcgaaa
     5341 ccaaattcgt tgttcgtacc ataggtgata tcagcaagat aggcatttct gcgttcagga
     5401 ctattgggtt gataaagatc gatacaatca acgcgcatac cgaggaaatt aaaaatcggt
     5461 ccgttccatt cagcatcgcg acgagcgaga taattattta cggttacgat atgcacacct
     5521 tcaccggcaa gagcatttaa gtaggcaggc aatgtagcta ccagcgtttt accttcgccc
     5581 gttgccatct cagaaatttt tccctgatgc aacacaatac caccgatcaa ctgtacatca
     5641 taatgcacca tgttccatgt gataggcaca cctgcaggct cccagctgtt tttgtatatc
     5701 gccttatcac catcgatcac aacaaaatca tgcgcaactg aaatcttttt atcgagttcg
     5761 gtggccgtag aaatgatttc ggtattttca gtaaatcgtc gagcagtttc tttcattacc
     5821 gcaaatgctt ccggcaataa tttgagaagt atttcttcaa tcaatttatc gcgatccttg
     5881 cgcaaagcat caatttgctt gtatatctca tccttttgat caacatcttc caatgtggga
     5941 tcatcagatt gaattttgag gctgctgatg cgattatcaa tttccgataa atgtgccttt
     6001 atcctaccct taaattcatc tgttttagca cgcagctggt cattcgacag ggtgctgagt
     6061 ttggcaaatt cgcggttgat gtcatccacc aatggcatga tggatttaat gtctttttca
     6121 tacttgttac ctccaaacaa tttggagatc atattggtga gtacactcat gctatactat
     6181 tgaaggtata tctgatgtca aaagttgttc cgaaatggcc tgcaaagata cggattaaca
     6241 cccttttaag actttttctt gccgatttgt atccgctact ccgggtattg gggtcgtgaa
     6301 accacggttt agtaaggatt gttatagctt tacagcacca aatccgcatg ccggcgacca
     6361 ttcagcaaat atcagcactc ttaggtcaac agggacaaat tgtcagtccg caagcgcctg
     6421 ttatacaggt attgacagat tccagaacaa ttttctcggg tgccgatagt ctgttttttg
     6481 ccatcagcgg ggctcagcac aatggtttac aatatgtaga acaggcatat gcggcaggtg
     6541 taagaagctt cgtggttgaa aaattaccct caacgctgca tggtgatgcc aattatttgt
     6601 tggttgaaaa cgccatcgat gcattacaaa tcatcgccgc agcacatcgc ttacagtttc
     6661 aatttccggt gatcgggatt accggcagca atggaaaaac aattgtaaaa gaatggcttt
     6721 accatttgct gaagcaacag ttggatattg tgcgcagtcc gaaaagctat aattcgcaga
     6781 taggcgttcc gctgagtgtg tggaaaatgg atgagcggaa tgagcttggc atttttgaag
     6841 cgggtatttc acaacccggt gaaatgcaga aattacagca agtgatcagg cctacgcatg
     6901 gtattatcac caatatttcg gatccgcatc gcgaaggttt tgaaaatgac gcacagaaat
     6961 tatcggagaa attacagttg ttcaaagaag catcgtcatt agtgtattgc gccgatcacg
     7021 aacaaatttc tgatgctatt aaaattatcc tgccaaatac aaaaacgatt gcatggagtg
     7081 cacgtagtaa taatgctgat gtaattatca gtgacatcga gcaactgccc gacagcacga
     7141 tgtttacgtt aaaatacgat gagcataatc tgcattttaa aattccatat agtgatagtg
     7201 catcattaga aaacagcatt catgcaatag taatgtcgct ggtgataatg aatgaaagaa
     7261 aaacacttgg tgcttttact atcagcaaca tcaccataca agcggcaacc ttgcctgttg
     7321 taagcatgcg attggaatta aaacgagccg accacaatag tctgctcatt aatgatgcat
     7381 acagtgcaga tattgcatcg ctggaaatag cattgaaatt tcaagcgcaa cacagcacag
     7441 ggttgcagcg cgtagtgata ttatcggatt ttgaccaaag cggattggaa cgccaattat
     7501 tggttggtga aatactaaaa ttactttctg cgtatcaagt cgaccgtatt attggcattg
     7561 gggaaggatg gcatgcaaat aatgaaattg acaaatcgcg cgtgcaaaca tttgcagata
     7621 ccgaaacatg catagcttat ctgcagcaac aaccgatatc aaatgccgtt attctgatta
     7681 aaggtgcaag gcgatttacg ttagagcgta tcggtcagct gttggaatta aaaacgcatg
     7741 gtacagcatt gcgcattcat ctcgatcaca tggtgagtaa tttgcaagtg tatcgctcaa
     7801 tattgaagcc cggtgtgaag acaatggtga tggtgaaagc atttagctat ggcagcggac
     7861 aggcagagat agcaaggctt ctggcgcatc agcgggttga ttatctggca gtagcctatg
     7921 ccgatgaagg tgttgatttg cgtcgacaag gcatacgctt gcccatcatg gtaatgaatc
     7981 ctgaaccgga aacttttgat caattattgg atgcacaact ggagccggaa atttatagta
     8041 tgcgtattct taatttatgg ttgcaggcag tacaaaacag aaaagcgcat tcagaaaaca
     8101 agcaatatgt tgtacatatc aaactcgaca ccggtatgca cagacttgga tttgaagcat
     8161 ctgatattga tgtattaatt gatacactgc aacaacatcc gcatatccat gttgcaggaa
     8221 ttttcacaca ccttgctgca acggatagtg ccgatcatca tgctttcacg aaattgcaat
     8281 tgcagaaatt tgaaagcatg tcgacaagaa ttactgatgc acttggttat gctgtaattc
     8341 gtcacgcatt aaattcttca ggcatcagta attatcctga agcgcagttt gatatggtac
     8401 gattgggcat cggactttat ggtgtcgatc catcgtcaac agtgcaatct cgattaaaac
     8461 atgtgagtac attggtaacc aatatagcac agattcgcaa tattcaggaa ggtgaaagtg
     8521 ttggatacag ttgttccttc atcgcaccaa aacccatgcg tatcgccacc attaa
//
LOCUS       DAOQOU010000142         8559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4514_length_8559_cov_5.450259, whole genome shotgun sequence.
ACCESSION   DAOQOU010000142 DAOQOU010000000
VERSION     DAOQOU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4514_length_8559_cov_5.450259"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLM90_11310"
     CDS             <1..563
                     /locus_tag="PLM90_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ90010.1"
                     /translation="LPSHLDLVGAEIELINHPNRERILKSVLDEVKNNYDFIFIDCSP
                     SLGLITVNALTSADSVLVPVQCEYFALEGLGKLLNTIKIVQNRLNNPLQIEGILLTMY
                     DARLRLSNQIVTEVKRHFADLVFDVIIHRNTKLAEAPGFGKPVVMYDAECTGAINYLN
                     LAREILQKNSATPSQLGNELLNIHQN"
     gene            637..1530
                     /locus_tag="PLM90_11315"
     CDS             637..1530
                     /locus_tag="PLM90_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ90011.1"
                     /translation="MSQKKPDLGKGIRALLQSMEDEKTDPIVTQKKTQELSGTSNIAI
                     GNIELNPFQPRSDFDETALKELSESIKVHGVIQPITVRTVGTNKFQLISGERRLRASK
                     LAGLTDIPAYVRNANDQEMLEIALIENIQREDLNAIEIAIHFRRLLDECNLTHEALAD
                     RLGKDRSSVSNYLRLLKLPPDIQRGLKEKLISMGHARALISLEDPIAQLAVYKEAIAG
                     ALSVRDVEALVRNYQPKKQKSAKSQTKDTSFAVRKLQDELSSHLATRVIIKPGSAGRG
                     EIVISYFSDDDLYRLKEIIEG"
     gene            1534..2157
                     /locus_tag="PLM90_11320"
     CDS             1534..2157
                     /locus_tag="PLM90_11320"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMZ90012.1"
                     /translation="MRHLLITLLCVFAASQMHAQQDSIMTQDMVDTTLAQTDSLHTPF
                     YKNLWSHDPHSVGRAALYSAVIPGLGQAYNGKYWKIPIVYGALGTSGYFIYYWYDFFD
                     ELRTAYIYRTDEDPLTIDTQYDYIPSDELLLQYVSQSKRYLDVMVIVTAAIYTLNIID
                     AVVDAHLYDFNVSDDLSMQVQPHYFFGYNQINPLQGTVGISLQLSLK"
     gene            2154..2873
                     /gene="dapB"
                     /locus_tag="PLM90_11325"
     CDS             2154..2873
                     /gene="dapB"
                     /locus_tag="PLM90_11325"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMZ90013.1"
                     /translation="MKVALIGHGKMGKAVEQVMHRFPNAHIAAIFSSSNGHRLSVDAL
                     KGIDVAIEFTTPLTAVAHIKLCIDAGVPVVTGTTGWYQSLPEITDYCKQKNGALLYAA
                     NFSIGVHLFLAANARLAALMQHQHQYTLNISETHHTQKLDAPSGTAIRIADTISEHAP
                     RYKGWVLEPEQKEHAIPITAHRIDHVPGIHTVEWKSSVDTITLTHSAQSREGFAEGAW
                     LAAQWIIGKQGVFSMQDVLGI"
     gene            2903..4375
                     /gene="dacB"
                     /locus_tag="PLM90_11330"
     CDS             2903..4375
                     /gene="dacB"
                     /locus_tag="PLM90_11330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HMZ90014.1"
                     /translation="MKKIISFFFILVLSCGVFAQTKIDSIGLNKLKGGLKAMSVDADL
                     VHANWGFCLMDPTTGKVLAEYGSEKSLTPASSQKVITTIAAMNILGADYTYKTYVEYT
                     GTIGKDSVLTGDLYIRGTGDPTLGSMRIDSLVTYDSLLEVWALKIKEKGIATINGKII
                     ADASYFEDYTTVGSWNWDDIGQYYGAGASGLNFAENKYTVYFSSNASRAKIDSIRPEI
                     DGMTVWNDVRVGGTGDEAYIYGAPDNYYRYVTGTIPANRKAYEVDGSMPDPPLFLALA
                     LKSALDTIGIHVTGQATTQYAVVRAGLADKSERKALFTHSSVPLKQMIFETNQHSINL
                     YAETFLKTIGKKQKNDGSREAGVNAENAFYSTAGLKLDAMHIEDGSGLSRLNYFSPKD
                     MCSILRFEMQQNSFTDFLPTLPVAGQSGTLKTMGDNTAAEGRIFAKSGSMYKVRSYSG
                     YVQTKSGQKLVFCVMVNNYTCGSAEIRAKLEKLMVLMAGV"
     gene            4454..5104
                     /locus_tag="PLM90_11335"
     CDS             4454..5104
                     /locus_tag="PLM90_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90015.1"
                     /translation="MRNISLLLICFIGFTACKNYTGIPDSFDYGRMDGNVYSNTYFDF
                     TFSMPDGWQVMENSVMDSLRRKTQEDLAEKDPELAKTVKAADVRTANLLTIICSKDPN
                     YFLYSANLNFVAENVKIAINVKDGKDYLESAKKNLGNLEYSLDFKGDIYKMDINGVEF
                     YVMNLINHYNGLDIKQSYYASIINGFAFTFVASWITDEQRTFIDEAISKMKFIKEA"
     gene            complement(5113..5487)
                     /locus_tag="PLM90_11340"
     CDS             complement(5113..5487)
                     /locus_tag="PLM90_11340"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HMZ90016.1"
                     /translation="MASTKSKFSVVLQNKCPRCQEGEMFVDPNPYHLNNMAKMHRNCA
                     KCGLRFEPETGFYYGAMYLSYALGVFSSLIIFAILNFVFRIPTLWAFIIVALSWIGLS
                     PMLFRFSRSLWLSLFTNRHRDI"
     gene            5551..7845
                     /locus_tag="PLM90_11345"
     CDS             5551..7845
                     /locus_tag="PLM90_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HMZ90017.1"
                     /translation="MKHIHAAILMLLIPSIAFSQKYVELYNAGKYEKAIDQAEKAIDK
                     NSKDLNAYLTKAMCNLHLYLDPVTQPAHQAGVLSAVNTLELIVKKDKDESFQQQHQPE
                     IDSILKAGGDMATKYFEKGDVSRADKLLADLITLQPRPEYYFLAGRIAQQADNDDLAI
                     HYYNTASAKIYKDSKAGKPVSEDFIPMFVALANAIGNQGDLKSAFVVYDRAFELFPSE
                     SLSDSCYYFLSALAEKHAYANDNIRIDQLIQYLDTIPAPVAKDSRFYALKWDCIFTRY
                     YNMQGYYIAANIREANSYLTQWACKDQFTAAADTLLSAMLRNTYLKMNVYEIAVSRDP
                     DMLQDYLTMKSCLNNGNMQQAEASYFDKIVYALEIKDFTFAGKLIYNLSSIKAYATQS
                     KAFEKQLFTGLQQLSFDELSQIDLYALSLMFPGNTVGKKLQKDESIRQITALIDKGDF
                     TNAGIKVRAQMRLDPKDMTVRTLYKTWVIKDYVTTYYNSATYSDLSVWNGNISQCDPG
                     KLPDSIHNKVLMRLNYYRRLVGIPDNCVFDKELNKKCQAAALMMTANHDLSHGPPPDW
                     SCYSPEGATGAGNSNLSLGYGGAEALSGQVEDDGGNNGAVGHRRWILNPFRKVYGHGS
                     TDDAMALWALGGNDADQPKEIREQYTDTYVAWPPEWYSPAPLLPARWSLGMYHADFSE
                     AKVQMFQGNNEIGLEVLPLEYGYGLSTIVWEPGINMYGAKGDQTYKVVVSHIGIDSTW
                     DEATQSYKKEYVDHTYYVIAVQMQ"
BASE COUNT         2563 a         1694 c         1868 g         2434 t
ORIGIN      
        1 tattgccatc acacctcgat ttagtgggtg ctgaaattga attaattaat cacccgaatc
       61 gcgaacgcat tttgaaaagt gtactcgatg aggtgaaaaa taactacgac ttcattttta
      121 tcgattgttc accttcattg gggcttatca cagttaacgc acttacatct gccgattcag
      181 tgctggtgcc tgtgcagtgc gaatactttg cgcttgaagg tctcggaaaa ttgttgaata
      241 caattaaaat tgttcaaaac cgattgaaca atccattgca aatcgaaggc atactgctta
      301 ctatgtatga tgcccgtttg cgactcagca accagatagt aacagaggtg aaacgccatt
      361 ttgccgatct ggtatttgat gtgatcatcc atcgcaatac caagctggcg gaagcaccgg
      421 gattcggaaa gccggtggta atgtacgatg ctgaatgcac aggggctatc aattacctca
      481 acctggctcg cgaaatcctg caaaaaaaca gcgcaacgcc tagtcagctc ggtaatgaat
      541 tgctgaatat tcaccagaat tgattatatt tattcaggta tgcttcccgg taaataattt
      601 ccgggagaat atcgttttat agcattaaca gaaatcatga gccagaaaaa accggatctt
      661 ggtaaaggta ttcgtgccct gctgcaaagc atggaagatg agaagaccga cccaatcgta
      721 acccagaaga aaacacagga gttgtcgggt acttcaaata ttgccatcgg taatattgaa
      781 ctcaatccat ttcaaccgcg ctctgatttc gatgagactg ccttaaaaga actcagcgag
      841 agtattaaag tgcatggcgt aattcagcca atcaccgttc gcaccgttgg caccaataaa
      901 tttcaattga ttagcggtga gcgtcgtttg cgtgcatcta aattggcagg attaacagat
      961 atccctgctt atgtgcgcaa cgccaatgat caggaaatgc tggagatcgc tttgatagag
     1021 aatattcaac gcgaagacct caacgccatt gaaattgcca tacatttccg acgtctgctc
     1081 gatgaatgta atctcaccca cgaagcactg gctgataggt taggaaaaga tcgctcgtct
     1141 gtaagtaact acttgcgatt attaaagtta cctccggata ttcagcgcgg attaaaagag
     1201 aaactcattt ccatgggaca tgcacgtgcc cttatttcac ttgaagatcc gattgcgcag
     1261 ttagcagttt ataaagaagc aattgccgga gcattgtcgg ttcgcgatgt ggaagcattg
     1321 gtgcgcaatt atcaaccgaa aaaacaaaaa tctgccaaat cgcaaaccaa ggatacttca
     1381 tttgcggtac gtaaattgca ggatgagctt tcgtcgcatc ttgcaacccg tgtaatcatt
     1441 aaacccggaa gtgcaggaag gggagagatc gtcatttcct atttttctga tgatgatctg
     1501 tatcgactaa aagaaatcat cgaaggataa ttcatgcgtc atttgcttat aaccctgtta
     1561 tgtgtgtttg ctgcaagcca aatgcatgca cagcaagata gcataatgac gcaagatatg
     1621 gttgatacaa cattggcaca aacagattca ctgcatacac cattttacaa aaatctatgg
     1681 tcgcatgatc cgcacagtgt tggtcgcgct gccttgtata gtgcagttat tccggggttg
     1741 ggacaagcat acaacggtaa atactggaaa attccaattg tttatggagc tctcggcacc
     1801 tcgggctatt tcatatatta ctggtatgat ttttttgatg aattgcgcac ggcatatatt
     1861 tatcgaacag atgaagatcc attgaccata gatacgcaat atgattatat cccatccgac
     1921 gaattattat tgcaatatgt atcgcaatca aagcgctacc tggatgtgat ggttatcgtt
     1981 acagctgcta tttatacatt aaatattatt gatgccgtag ttgatgcgca tttatatgat
     2041 ttcaacgttt ctgatgatct ttcgatgcaa gtgcagccgc attatttctt cggttataat
     2101 cagataaatc cactgcaggg aactgtgggt atttcacttc aattgtcgct aaaatgaaag
     2161 tagcactcat tggacacgga aaaatgggaa aggctgtaga gcaagtaatg cacagatttc
     2221 cgaatgcaca tatcgctgcc atattttcct cgtcgaatgg ccacaggctt tcagttgatg
     2281 cactgaaggg aatagatgta gccatagaat tcacaacgcc attaactgca gttgctcata
     2341 ttaaattatg catcgatgcg ggtgttcctg ttgttacggg gacaaccggt tggtatcaat
     2401 cattaccgga aattacggac tattgtaagc aaaaaaatgg cgctttattg tatgcagcga
     2461 atttcagcat aggggtgcat ttatttctgg cggcaaatgc gcgacttgcg gcattaatgc
     2521 agcatcaaca tcaatatacg ctcaatattt cagaaacgca tcacacacaa aaattagacg
     2581 cgcccagtgg cacggcgatt cgcattgccg atacgatcag tgagcatgct ccgcgatata
     2641 agggatgggt tttggagcct gaacaaaaag aacatgcaat cccaataaca gctcatcgta
     2701 tagaccatgt tcccggtata catacggttg aatggaaatc gagtgtggat accattactc
     2761 ttacccattc agctcaatca cgagaaggct ttgctgaagg cgcttggttg gctgcacaat
     2821 ggataatagg aaagcaaggt gtttttagca tgcaggatgt tttggggata taattgttgt
     2881 aactaattgc gtttaataag gcatgaagaa aattatttca ttctttttca ttttggtttt
     2941 atcctgtgga gtttttgccc agacaaaaat tgattcaatt gggttgaata agcttaaagg
     3001 tggattaaaa gcaatgtcgg tagatgcaga tcttgtgcat gccaactggg gattttgtct
     3061 gatggatcct actaccggaa aagtacttgc tgaatatggc agtgaaaaaa gccttacacc
     3121 tgcttcaagc caaaaagtta ttacgacaat tgcggccatg aatattttgg gtgccgatta
     3181 tacttacaaa acgtatgtcg aatataccgg taccataggt aaggattctg tgctcaccgg
     3241 tgatctctat attcgtggaa ccggtgatcc taccttggga agcatgcgca ttgattccct
     3301 ggtaacttat gattctttgc tggaagtatg ggctttgaaa attaaggaaa aaggtattgc
     3361 gacaatcaat ggaaaaatta ttgctgatgc ttcttatttc gaagattata caacggtagg
     3421 gagttggaat tgggatgaca tcggccagta ttacggagcc ggtgcatcgg gattaaattt
     3481 cgctgaaaat aaatatactg tatacttctc ttcaaatgct tcgcgtgcaa aaattgacag
     3541 cattcgtccg gaaattgatg gcatgactgt gtggaatgat gtgcgtgtcg gaggaacagg
     3601 agatgaagca tatatttatg gcgctcctga taattattat cgatacgtaa ccggcacaat
     3661 tcccgcaaac agaaaagcgt atgaagtgga tggcagtatg cccgatccgc cattatttct
     3721 tgccctggca ttaaaatcgg cacttgacac aattggtata catgttacgg gacaggctac
     3781 cacgcaatat gccgtagtgc gcgcaggact ggcagataag tctgaaagaa aagcattgtt
     3841 tactcatagc tccgtgccac tgaaacaaat gatatttgaa acaaatcagc atagcatcaa
     3901 tttgtatgcc gaaacatttt tgaaaacaat tgggaagaaa cagaaaaacg atggcagtag
     3961 agaagcaggt gtaaatgccg aaaatgcatt ttattccacc gccggattaa aactcgatgc
     4021 catgcatatt gaagatggca gtggtttgtc gcgactaaat tatttttctc caaaagacat
     4081 gtgtagcatt ttgcgttttg aaatgcagca gaattcattt actgattttc tgccaacctt
     4141 acctgttgcg ggccagagcg gaactttaaa gaccatgggc gataataccg cagcagaagg
     4201 ccggatattt gccaaaagtg gttccatgta taaagtgcgt tcatactcag gatatgtgca
     4261 aacaaaaagc ggacagaaac ttgtattttg tgtaatggtg aataattaca cctgcggatc
     4321 tgcagaaata cgcgctaaat tggagaagtt gatggtattg atggcaggtg tctgatttac
     4381 ctgtaccata agttttatct aacctcattg cataccaatc tatattgtcg tagatttatt
     4441 caaaatctga aatatgcgga atattagttt gcttttgata tgttttattg ggttcactgc
     4501 ttgtaaaaat tatacaggaa ttcctgattc atttgattat ggtcgaatgg atggtaatgt
     4561 gtattccaat acctattttg attttacatt ttctatgccc gatggttggc aggtaatgga
     4621 gaattctgtg atggacagtc ttcgcagaaa aacacaggaa gatctggcag agaaggatcc
     4681 tgaattagca aaaacagtaa aagccgcaga cgttcgaaca gcaaatctgc tcacaatcat
     4741 ttgcagcaaa gaccccaatt atttcttata cagtgctaat ctgaattttg ttgcggaaaa
     4801 tgtaaaaatt gccattaatg taaaagatgg caaggattat cttgaatctg caaagaagaa
     4861 tttgggcaat ttagaatatt cgctggattt taaaggagat atctacaaaa tggatatcaa
     4921 tggtgttgag ttttatgtaa tgaatttgat taatcattac aacggacttg acatcaaaca
     4981 atcgtattat gcctcaatta ttaatggctt tgccttcaca tttgtagctt catggattac
     5041 agatgaacag cgcaccttta ttgatgaggc tataagtaaa atgaagttta ttaaagaagc
     5101 ctgaaatgaa aatcagatat ctctatgcct gttagtgaat aaacttaacc acagtgatct
     5161 gctaaatctg aaaagcatgg gtgataatcc tatccatgat aaggctacaa tgataaacgc
     5221 ccataaggtt ggtatcctga aaacaaagtt gagaatagcg aatattatca agctggaaaa
     5281 aacaccaaga gcatagctca aatacatcgc tccataataa aatcctgttt ccggttcaaa
     5341 gcgcaagccg catttcgcgc agttgcgatg cattttcgcc atattgttga ggtgataggg
     5401 attcgggtct acaaacattt caccctcctg acaacggggg catttgttct gtaaaacgac
     5461 actaaatttt gatttcgttg aagccatgcg caaaattacc ctatttaagt gcaaaatagg
     5521 atagtacctt tgtgcgttta gtcactgaag atgaagcaca tacacgcagc tatcctaatg
     5581 ctgcttattc cgtcaattgc ctttagccag aagtacgttg aattgtacaa cgccggtaag
     5641 tacgaaaagg ccatagatca ggctgagaag gccatagaca aaaacagcaa ggacctcaat
     5701 gcctatctga caaaggccat gtgcaacctc catttatacc ttgatcccgt aactcagccc
     5761 gcacatcagg caggcgtatt gtcagccgta aatacactcg aactcattgt caaaaaggat
     5821 aaggacgaat catttcaaca gcagcatcaa cccgaaattg acagcatttt gaaagccggt
     5881 ggcgatatgg caacaaagta ttttgagaag ggagatgtat cacgggcgga taaactgctt
     5941 gccgacctta tcaccctgca gccacgaccg gaatactatt ttctggccgg tcgcattgcg
     6001 cagcaggcag ataacgatga tctcgctatc cactattata acaccgcttc ggcgaaaatt
     6061 tataaagatt caaaggcggg caaacctgtt agtgaagatt tcattccaat gtttgttgca
     6121 ctagcgaacg ccatcggtaa ccagggagat ttgaaatcgg cattcgtagt gtatgatcgt
     6181 gcctttgaat tatttccttc cgaatcgctt tcagattcat gctattattt cctgagcgct
     6241 ttggctgaaa aacatgctta tgctaatgac aatatcagaa tagatcaact catccaatat
     6301 cttgatacca ttcccgctcc cgttgcaaag gattcgagat tttatgcatt aaaatgggac
     6361 tgtattttca caaggtatta caacatgcaa ggatattata tagctgccaa tatcagagaa
     6421 gcgaacagct atctcaccca atgggcgtgt aaagatcaat tcaccgctgc cgctgatacc
     6481 cttttgtctg cgatgttaag aaatacatat ctgaaaatga atgtatatga gattgctgtt
     6541 tcccgcgatc cggatatgtt gcaagattat ttaaccatga aatcctgcct gaataatgga
     6601 aatatgcaac aagctgaggc ctcttatttc gataaaattg tttacgcatt agagataaaa
     6661 gattttacgt ttgccggaaa actcatttat aacctgtcat caataaaagc ctatgccaca
     6721 cagtcgaaag cttttgaaaa acaattattc accggtttgc agcaattatc atttgatgaa
     6781 cttagtcaaa tcgatctgta tgcccttagc ctgatgtttc cgggtaatac cgttggcaag
     6841 aaattgcaga aggatgaatc tattcgtcag atcacagcac tcatcgacaa gggtgatttc
     6901 acaaatgcag gcataaaggt acgtgctcaa atgagattgg atcccaaaga catgaccgtg
     6961 agaaccttat ataaaacctg ggtgatcaaa gattatgtca ccacatatta taatagtgca
     7021 acctatagtg acctgtcggt atggaatggc aatatttcac agtgcgaccc tggtaagctg
     7081 ccggattcta ttcataataa agtgttgatg cgattgaatt attatcgcag gcttgtaggg
     7141 attccggata attgtgtttt tgataaagaa ttaaataaaa aatgtcaggc tgccgcatta
     7201 atgatgaccg caaatcatga cttgtcgcat ggaccacctc ccgattggag ttgctattca
     7261 ccggaaggtg ctaccggtgc cggaaattca aatttatcat taggatacgg tggtgctgag
     7321 gcgttatcgg gacaagtgga agatgatgga ggtaataatg gggctgttgg tcaccgcaga
     7381 tggattctaa atccatttag aaaagtttat ggacatggat ctaccgatga tgccatggcc
     7441 ttatgggcgc ttggtgggaa tgatgcagac cagccgaagg agattcgtga acagtatacg
     7501 gatacctatg ttgcctggcc tcctgaatgg tatagtcccg ctccattgct tcctgcccgc
     7561 tggtcgctgg gaatgtatca tgccgacttt tcggaagcta aagtgcaaat gtttcaaggc
     7621 aataacgaaa taggactcga ggtgctaccg cttgaatatg gctatggatt gtcaaccatt
     7681 gtttgggagc cgggaatcaa tatgtatggc gctaagggag accaaacgta caaagtggtt
     7741 gttagtcata ttggtatcga ttccacttgg gatgaagcca cacagtccta caaaaaagaa
     7801 tatgtcgatc atacctatta cgtgatagca gtgcaaatgc agtaatttta ttttttaaaa
     7861 ctgcaactgc tgacctctac acttgccata ttacttttct tgctcatcgg ttttggcggg
     7921 gtagggtacg atgctttcaa aggactgcgt taccgcattt gggataccgt ctatttgata
     7981 tgcatttgcc ttggcattta tagcacatac aatcgctttg gccagctttg gggtaatatc
     8041 ctcgttttgg cttccgcggc aatcctcgta gcacttttcc tcctacatcg ccgccgttat
     8101 cgcaggtaac ataaatttcc agcctaaacg taacttgttg atttataaat acttgtgcga
     8161 aatattttga aatattcaat ccatttagta cctttgcact ccatttggcc tcgtagctca
     8221 actgaatacc cgccttgcta agccgtatgc taagcaggcg aggcaggccc gccttgctaa
     8281 gccgtatgct aagcaggcga ggcaggcccg ccttgctaag ccgtatgcta agcaggcgag
     8341 gcagggagca tctgactacg gatcagaagg ttacattgag atttatacgc tctcccctta
     8401 ttattcctag tatattttcg tggccattat tttggtttag acaaataatc cgaagagttt
     8461 aggatttatg ccatgtcaga gcatcctgtt gtctattttg aattttactt aaatgacatt
     8521 atctttgcac tccatttggc ctcgtagctc aactgaata
//
LOCUS       DAOQOU010000143         8440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4605_length_8440_cov_6.516756, whole genome shotgun sequence.
ACCESSION   DAOQOU010000143 DAOQOU010000000
VERSION     DAOQOU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4605_length_8440_cov_6.516756"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1258
                     /locus_tag="PLM90_11350"
     CDS             182..1258
                     /locus_tag="PLM90_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90018.1"
                     /translation="MQKWMVAGGLALAALTNSCSTDFDLNADFIETPVIYGLLDASSD
                     TQFIRINRAYLSDEIDGLTMAADPEAIYYGDELEARIEEYSGGTLLHTYILERVDGDT
                     LGLPKADGIFANHPNILYRFIADLDYTHTYKLFAINNETTKSVEATTQLVDSFAIIRP
                     DDESVFPQAISLAPQTVYQLRWRSSQDAKIYDLTMRFHYREGIYYEDGDSVHYTFSGY
                     KDWKMETNYTAESTDGGLILTYDVPGETFYNFIKDSFEPVADNNFIRIADSVQFFIDG
                     GGEELFNYFEYNNATLGITEGQITPAYTNVFGGLGILSSRYHKEGLIYPFAIQTRDSI
                     ACSSVTSGLNFAPDGSTFGFPYCQ"
     gene            complement(1315..2373)
                     /locus_tag="PLM90_11355"
     CDS             complement(1315..2373)
                     /locus_tag="PLM90_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90019.1"
                     /translation="MRRLLGIIAFCILPFFQWRCDNTLQINGNYEEKVVVYGLLNYGQ
                     DTNYIRIQKTFLGENTSALNLASQAGENYYSAGELEVKLEQWKFGALVNTTFCDYVDG
                     DTLGIEKDSGVFASSPNILYRICMPLDSSSTYKLHILRLEDSSIFTAETNLVQSFYLF
                     SPTKPNAYISFADTGNITYTCKQAVNGMIYDLVMTFRYAEVRHINGDSTIHSIDWVIF
                     DNKEGTGTIGISNLNYTLSRHAFYSFIASSLEVDTAVTRYAININYAWYAGGIELYDQ
                     YLNLLANLGLNEDYISPEYTNVNGGLGIFSSRYIEAANNLILDDISLDSLSCGAITRQ
                     LRFVGSASNHDYPACWEQ"
     gene            complement(2492..4876)
                     /locus_tag="PLM90_11360"
     CDS             complement(2492..4876)
                     /locus_tag="PLM90_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HMZ90020.1"
                     /translation="MSKKTKRILLITVGVLVLLAGASYAMKGKIKNMVKGKDNKRVAA
                     QQSADIKEVAWDDSKTGNEAMIEYLSKVYDQVNKPGLRFMNTAILNSLLAQPIPQDPV
                     GMYTWYFDVCNQMLNAGKVNEAVEKTKEFEAKPQFAKLSPKELGGWYYTKGLVALRFG
                     EVENCIGLHNSESCIWPLSQAAQSAKKDGPNMAVEAFTKCLQYDKENLSAMWLMNVAY
                     MQLGTYPDAVPSQWLIKPESIKSEYDVPKFKNIAIDLGVDNPNMCGGVILDDFNNDDL
                     IDIFTCGWGLKERSFLLLNKGDGTFDDISTKAGLDRYPGGLMIQQTDYNNDGNLDVWV
                     SRGAWYSNFGILPSSLLRNNGDSTFTDVTREVGIWTTRPSQAATWADFNNDGWLDLFI
                     GNEASRKGDKINDCQLWINEKGKFHNIAKEAGVDVNSFVKGVSSGDYDNDGDPDLYIS
                     ANGFKNYLFRNDTKKGSAKMKFTNVTKEAGVVGPESSFPCWFFDYNNDGWLDIMNFSY
                     SAGISDNDIPAEYMGKPTESDKTALYRNNGDGTFTNVNEETGLAKRTFLVMGSSYGDF
                     DMDGWIDFYVGTGKPDMRSLTPNRMFRNAEGKYFQDVSTSAGIGVLQKGHEISFADLN
                     NDGYPEIFAQMGGAYEASGFYDCLFENPASFGNNWISIDLTGTTTNKCARGARIKITT
                     IENGKERNIYEWVTQGSSFGANSLRQEIGIGKATAIKSIEISWPTSGKKTILKDVPMN
                     QFIAVTEGTEGFTKINLTPFVFQSTPEKSFTHKMMETMNMGNDRYMNMQHDMGH"
     gene            complement(5022..7304)
                     /locus_tag="PLM90_11365"
     CDS             complement(5022..7304)
                     /locus_tag="PLM90_11365"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HMZ90021.1"
                     /translation="MRSTSLVLFSSLSLVILFSAGCGKDKKQREFAQNTVVYDVNLPS
                     NEAMAKYLAECNEKYFKNTHNRFASTTRALDFESKPAPKEAKKFVEWKKAIGDEWLFA
                     GDTKKSLAAYEEAMKKVQDEGMKGKLVDDIRYMQAVAYMRLGEQLNCIANHVSESCIL
                     PIAQPAWYVEKDPTIKAVAIWEEILKQHPDDLSAKFLLNIAYMNLGKYPDGMPTEYRI
                     DPNEFKSEYPLQRFPNIAANLGVDFITKSGGVVVEDFNNDGLLDIAASGWFLDEQLKI
                     MINNGDGTFTDNTEVSKLKGITGGLDLKCADYNNDGFMDILIPRGAWWNDFGKLPASL
                     LKNNGDGTFTDVTYETGLMKHLRPTQSAVFADFNNDGWLDIYFGNESRRDSEPYYNEL
                     FLSDGKGKFTEVGKEAGVDVICFSKGVVSGDYNNDGWMDIAISSQGTENYLFRNDTGK
                     NGGKVKFTDVTKEAGVSKPIKSFPIAFLDYNNDGWEDLFVCTYDADNCDYDNAAEYFN
                     GTPKGEYSILYKNNGNGTFTNVTKDMNLQVAMTVMGLNYGDLDNDGWLDIYCGTGTPN
                     YTSLVPKRMFRNNGGVGFQDVTTAGGFGHVQKGHGIGFGDVDNDGDQDVYEDMGGGYE
                     GDGFQGAFYENPGNTGNNWITLQLVGTKANQCAIGSKVKITVEEGGKTRDIYQVVSSG
                     ASFGVNSIRLETGLGKATAIKQIEVTWQGPNTKQVFTNVPMNNFVKLTEGVDAFELQT
                     LQPFKFSLDNHSTMDHSKMM"
     gene            7446..8273
                     /gene="panB"
                     /locus_tag="PLM90_11370"
     CDS             7446..8273
                     /gene="panB"
                     /locus_tag="PLM90_11370"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMZ90022.1"
                     /translation="MSSAQPDFNKVKRVTVHTLQEMKEHGVKISMLTAYDFSMARILD
                     DAGVDVLLVGDSASNVMAGHETTLPITLDQMIYHAASVVRAIDRCLVVVDLPFGSYQG
                     NSKVALDSTIRIMKESGAHAVKLEGGREVKESIERILSAGIPVMGHLGLTPQSIYKFG
                     TYTVRATEEMEAQKLYEDAQLLEQLGCFSLVLEKIPASLGEKVAAKLHIPVIGIGAGG
                     GVDGQVLVMHDMLGITKEFKPRFLRRYLNLYDDIKKGVSQYIADVKSNDFPNDMEEY"
BASE COUNT         2391 a         1812 c         1624 g         2613 t
ORIGIN      
        1 tacacctata cgctggtggc tgacggcgta gtagttggca ctaagttttt gagtgttgcc
       61 aaataatggc gcctggtata taggataaaa ggctctgcat ggctgcaggg ccttttttaa
      121 tagcatgatt cctgacagag ggcttacaat cccttctcca aatcagctat ctttgcgttc
      181 tatgcaaaag tggatggtag cgggaggctt ggcattagcc gcccttacaa acagttgttc
      241 aacagatttt gaccttaatg cagattttat tgaaacgcct gtgatttatg gtttattgga
      301 tgcgtcttcc gatactcaat ttattcgcat caaccgcgcc tatttgagcg atgagatcga
      361 cggtcttaca atggctgctg atcctgaggc tatctattat ggcgatgaat tagaagcaag
      421 aatcgaggaa tactcgggcg gaacattatt gcatacttac attcttgaac gtgtagatgg
      481 cgatacctta ggcttgccga aggcagatgg tattttcgcc aatcatccga atattttgta
      541 tcgttttatt gcagatttgg actataccca tacctacaaa ttatttgcaa tcaataatga
      601 aacaacaaaa tctgtagaag ctactacaca gttggtcgac agctttgcaa tcattcgtcc
      661 ggacgatgaa agtgtttttc cacaagcaat ttcattggca cctcaaaccg tataccagtt
      721 gcgctggaga tcatcacaag atgcaaaaat ctatgacctt accatgcgct ttcattatcg
      781 tgaaggtatt tactacgagg acggtgatag cgttcattat acattctcag ggtataagga
      841 ttggaaaatg gaaacgaatt acaccgctga atctactgat ggcggattaa ttcttactta
      901 tgatgttccg ggagaaacat tttacaattt cattaaagac agtttcgaac ctgtagccga
      961 caataatttt attcgcattg cagattctgt gcaatttttc attgacggcg gtggcgaaga
     1021 gttgttcaat tacttcgaat ataacaatgc tacacttggc attactgaag gtcaaataac
     1081 accggcgtat acaaatgtat tcggtggtct tggaatattg agcagccgct atcataaaga
     1141 aggactaatt tatccttttg caattcaaac aagagattca attgcctgta gtagtgttac
     1201 aagcggactc aattttgcac cggacggcag cactttcggg tttccttatt gccagtgatt
     1261 gggttgattg gctaattgcc ataaatgctg aaaaccaatt cgcgcttcac aagatcactg
     1321 ttcccaacag gccggataat catgatttga ggcacttcct acaaatctca attgacgtgt
     1381 aatcgctccg cacgacagtg aatccagcga tatatcatct aatatgaggt tgttagccgc
     1441 ttcaatatat cgcgaagaaa aaataccaag accaccattg acattggtgt attcaggtga
     1501 gatataatct tcgttcaaac caaggtttgc caatagattc aaatactggt catataattc
     1561 aattcctcct gcataccagg cataattgat atttatagca tatcgggtga cggcggtatc
     1621 aacttctaaa gaactcgcaa tgaacgaata aaaagcatgt cgactcaatg tataatttaa
     1681 attactaata cctatggttc ccgttccttc cttgttatca aaaatcaccc agtcaatgga
     1741 atgaattgta gaatcaccat ttatgtgacg cacttctgca tatctgaaag tcatcaccag
     1801 gtcatagatc atgccgttaa cagcttgctt acaggtgtac gtgatattgc ctgtatcagc
     1861 aaaactgatg tatgcattcg gctttgttgg agagaataaa taaaaacttt gaacgagatt
     1921 ggtttcggca gtgaaaattg agctgtcctc tagtcgcaaa atgtgcaatt tgtaagtaga
     1981 agagctatcg agaggcatgc aaatccggta taatatattt ggcgaagaag cgaatactcc
     2041 ggagtccttt tcaatgccta aagtatctcc atcaacatag tcgcaaaatg ttgtatttac
     2101 cagtgcacca aatttccatt gttccagttt tacttctaat tcaccggcac tgtaataatt
     2161 ctcccctgcc tgagatgcaa ggttaagagc acttgtattt tcaccaagaa acgttttttg
     2221 tatgcggata taattggtat cctgaccata attcaataaa ccgtaaacaa caactttctc
     2281 ttcataattt ccattgatct gcagtgtgtt atcgcaacgc cattggaaga atggcaggat
     2341 acaaaaggct atgatcccaa gcaggcgtcg catattcaaa agtaatatta attgtaattg
     2401 attgccacct tcggcgaaaa aattacgcga ataaaacaaa aacgcccgtc aatttagaca
     2461 ggcgttttca atatgctaaa ctgtatagaa atcaatgtcc catgtcgtgc tgcatattca
     2521 tgtagcggtc gttacccatg ttcatagtct ccatcatttt gtgtgtaaat gatttttcag
     2581 gagtgctttg gaatacaaaa ggtgtgagat tgattttcgt aaatccttct gtaccttcag
     2641 taacagcaat aaattgattc attggtacat ctttcaaaat cgttttttta ccgctggttg
     2701 gccacgagat ctctatagat ttgatggctg tagccttacc gatacctatt tcctgacgaa
     2761 ggctgtttgc gccaaagctg gagccttgcg ttacccattc gtagatattg cgttctttgc
     2821 cattttcaat agttgtgatt ttaattcgtg caccgcgagc gcatttattt gttgttgttc
     2881 cggtcaaatc aattgaaatc cagttattgc caaacgatgc cgggttttca aataagcaat
     2941 catagaaacc tgatgcttca taagcgccgc ccatttgtgc aaaaatctcc ggataaccgt
     3001 cattgttcaa atcggcaaaa gatatctcat gtcccttttg caaaacacca attcctgcag
     3061 atgtagatac atcctggaag tattttcctt cagcattgcg gaacatacga tttggtgtaa
     3121 gcgaacgcat atccggttta ccggtaccaa catagaaatc gatccatccg tccatatcaa
     3181 aatcaccata gcttgatccc attaccagga aggtgcgttt tgcaaggcct gtttcttcat
     3241 tcacgtttgt aaacgtgcca tcaccattat tgcggtagag ggcagtttta tcactttcag
     3301 ttggtttgcc catatattca gccggaatat cattatctga aatgcctgcg ctatagctaa
     3361 agttcataat atccaaccaa ccatcattgt tataatcaaa aaaccaacat gggaagcttg
     3421 attcaggacc tacaacaccg gcttcttttg taacattggt aaatttcatt tttgcactac
     3481 cctttttagt gtcgttgcgg aaaaggtagt ttttaaagcc atttgctgat atatacaaat
     3541 caggatcgcc atcattatca taatcaccgc tgctcactcc ttttacaaat gaatttacat
     3601 ctacacctgc ttctttggcg atgttgtgga atttgccttt ttcgttgatc cataattggc
     3661 agtcgttaat tttatctcct tttcgcgatg cctcatttcc gataaataaa tccaaccaac
     3721 catcattatt aaaatcagcc catgttgctg cttgagaagg acgtgttgtc caaataccaa
     3781 cttcacgggt tacatcagta aatgtagagt ctccattatt acgtaataat gaacttggca
     3841 agataccaaa attgctgtac caggcgccgc gtgaaaccca aacatcgagg ttaccatcat
     3901 tgttataatc ggtttgttgg atcatcaatc ctcccggata tctatccaaa cctgcttttg
     3961 tagaaatatc atcaaaagtg ccatcacctt tattcaataa aaggaagctt ctttctttca
     4021 gaccccatcc acaagtgaat atatctataa gatcgtcgtt attgaaatcg tcgagaatta
     4081 cgccgccgca catatttgga ttatccacgc cgaggtcgat agcaatgttt ttaaatttag
     4141 gaacgtcata ttctgattta attgactccg gttttatcaa ccattgagat ggcactgcat
     4201 ccggataagt acccaattgc atataagcaa cattcatcag ccacattgca gacaaattct
     4261 ccttatcata ttgcagacat tttgtgaagg cttcaactgc catattcgga ccatcctttt
     4321 ttgcactttg ggcagcttgt gaaagtggcc aaatacaaga ttcagaatta tgcaagccta
     4381 tacaattttc tacttcacca aatcgtaaag caacaagtcc ttttgtataa taccaaccac
     4441 ccaactcttt tggtgaaagt ttagcgaatt gtggttttgc ttcaaattct tttgtttttt
     4501 caacagcctc attcactttt ccggcattca gcatctggtt acaaacatcg aaataccagg
     4561 tgtacatacc taccggatct tgaggaatag gctgagccaa taaactgttt agaatggctg
     4621 tattcataaa gcgcaaaccc ggcttattta cctgatcata aacttttgac aggtattcaa
     4681 tcatggcttc gttacctgtt ttagaatcat cccatgcaac ttccttaatg tcggcagatt
     4741 gttgggcagc aacgcgtttg ttgtcttttc ctttaaccat attcttgatc ttacctttca
     4801 tggcgtatga tgctcctgca agtagtacca aaacaccaac agtgatcaat agaatacgtt
     4861 ttgtcttttt gctcataaaa attgaaatat tgaggttaag tgtgatggtg caaagttctg
     4921 attttagcgg gcaccaatcg cttattagcc gccggtttgc tcaaaaattt tgtaaacggt
     4981 attaaaatat ttctttaaca gtttaactca atttcaggga tttacatcat tttcgagtga
     5041 tccattgtag agtgattatc caagctgaac ttgaagggct gtaaggtttg caactcaaaa
     5101 gcatccactc cttcggtcag ttttacgaag ttattcatag gtacattggt aaatacctgt
     5161 tttgtattag gtccttgcca ggtcacctcg atctgcttaa ttgccgttgc cttgcctaaa
     5221 ccggtttcca aacgtatgga gtttacgcca aaacttgcgc cggaagatac tacctgatat
     5281 atatccctgg ttttgcctcc ttcttcaacg gtaatcttaa cttttgagcc tatggcacat
     5341 tgattggctt ttgtccccac tagctgtaat gttatccagt tattccctgt attgcccggg
     5401 ttctcataaa atgctccctg gaaaccatca ccttcataac caccgcccat gtcttcataa
     5461 acatcctgat cgccgtcatt atctacatcg ccgaaaccga taccatgtcc tttttgtacg
     5521 tgaccaaaac ctcctgcagt tgttacatcc tgaaatccaa ccccaccatt atttcgaaac
     5581 atccgtttag gtacaagtga agtgtaattt ggagttccgg tgccacaata aatatccaac
     5641 caaccatcat tatctaaatc gccgtaattc aatcccatta ctgtcatggc tacttgtaaa
     5701 ttcatatcct ttgtgacatt tgtaaatgtg ccattaccat tatttttgta aaggatagaa
     5761 tattcacctt taggtgtgcc gttgaaatat tctgccgcgt tgtcgtaatc gcaattatct
     5821 gcatcataag tgcacacaaa taaatcttcc cagccatcat tgttataatc taaaaatgcg
     5881 atcgggaagc ttttaatagg tttactcaca ccggcttctt tagttacatc agtaaattta
     5941 accttaccgc catttttgcc ggtatcgttt ctaaataaat aattttctgt accctgactt
     6001 gaaatggcaa tatccatcca gccatcatta ttgtaatcgc cggacactac tcctttggaa
     6061 aaacaaatta catcaactcc tgcttctttt ccgacttctg taaatttacc cttcccgtca
     6121 ctaaggaata attcattgta atacggttct gaatctcgtc gggattcgtt gccgaaataa
     6181 atatccagcc aaccgtcatt attgaaatcg gcaaacactg cagactgcgt aggacgaaga
     6241 tgtttcatca atcctgtttc atatgtcaca tccgtgaaag tgccgtcgcc attgtttttc
     6301 agcagagaag cgggtaattt tccgaaatcg ttccaccaag cgccacgagg aatcaggatg
     6361 tccatgaatc catcattgtt ataatccgca cacttaagat caagtccgcc ggtaatgcct
     6421 tttagtttac ttacttcagt attatctgta aaagtgccat caccgttatt gatcattatt
     6481 ttaagttgct cgtcaaggaa ccaacctgaa gcagcaatat ctaataaacc gtcattatta
     6541 aaatcttcaa caacaacacc acctgatttg gtaataaaat ctactccgag gtttgcagca
     6601 atattcggga atcgctgcaa tggatattcc gatttaaatt cattcgggtc gatgcgatat
     6661 tctgttggca tgccatcggg atattttccc aaattcatat aggcgatatt caacagaaat
     6721 tttgcagata aatcatcagg gtgttgttta agaatttctt cccaaatggc aaccgcttta
     6781 atggtaggat ctttttcaac ataccaagcc ggctgtgcga taggtaaaat acaagattca
     6841 gatacgtgat tggcaatgca atttaattgc tctccaagac gcatataagc aactgcttgc
     6901 atataacgaa tatcatctac aagcttacct ttcatgcctt catcctgcac ttttttcata
     6961 gcctcttcat aagcggcaag cgattttttt gtgtcgcctg caaacaacca ttcatcacca
     7021 attgcttttt tccattcaac aaattttttc gcttctttcg gcgcaggttt gctttcaaaa
     7081 tccaatgcgc gtgttgtgct tgcaaagcga ttatgtgtat tcttaaaata cttctcgttg
     7141 cattcagcca ggtatttggc cattgcctca tttgaaggga gatttacatc ataaacaacc
     7201 gtattctgtg caaattctcg ttgctttttg tctttaccgc atccggcaga aaatagtatg
     7261 actagtgaca ggctgctaaa caatactaag gaggtgctgc gcataagggg tgaaattttg
     7321 tgcaaaggta ctgtgtttta accctggaag ggcatgttta agaaaagttt taatgtgcga
     7381 aaccccctga tacacaaagg ttcatgcaaa atatggtgta tctttccccc gaaaaatcta
     7441 gtgtcatgtc gtctgcccag cctgatttca acaaagtaaa gcgtgtaacc gtccacaccc
     7501 ttcaggaaat gaaggagcac ggggtgaaga tttctatgct tacagcctac gacttctcca
     7561 tggctcgcat ccttgatgat gccggggttg acgtgctttt agtgggcgac agtgcttcta
     7621 acgttatggc cggtcacgag actaccctgc ccatcacctt ggaccagatg atttaccatg
     7681 ctgccagcgt agtgcgtgcc attgacaggt gtctggtggt tgtagacctg ccattcggct
     7741 cctatcaagg gaattcaaaa gtggcgcttg attcaaccat ccgcattatg aaagaatcag
     7801 gggcgcatgc agtgaaactg gaaggtggac gtgaggtgaa agagtctatc gaaaggatac
     7861 tctctgccgg tattcctgta atgggtcacc ttggacttac accgcaatcc atctacaaat
     7921 tcgggacata tacggttagg gctactgaag aaatggaagc tcaaaaactg tatgaagatg
     7981 cgcagttact ggagcaattg ggctgtttct ctcttgtatt agaaaaaatc cctgcctccc
     8041 ttggtgaaaa agttgcagcc aaacttcata tcccggttat aggaattggc gcaggcggtg
     8101 gtgttgacgg tcaggtttta gtaatgcacg atatgctcgg tatcaccaaa gaattcaaac
     8161 cccgattttt acgcagatac ctcaatctat atgacgatat caaaaaaggt gtcagccaat
     8221 atatcgccga tgtgaagtcg aatgattttc ctaatgatat ggaggagtat tgagtggtga
     8281 gtggtgagtg gtgagtggtg aatcgtgagt cgtgagtcgt gagtcgtgag tcgtgagtcg
     8341 tgagtcgtga atcgtgaatc gtgaatcgtg atttttttac ctgatacttg atacctgata
     8401 cttgatacct gatacttgat acttgatacc tgataccaat
//
LOCUS       DAOQOU010000144         8307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4723_length_8307_cov_5.023873, whole genome shotgun sequence.
ACCESSION   DAOQOU010000144 DAOQOU010000000
VERSION     DAOQOU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4723_length_8307_cov_5.023873"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..667
                     /locus_tag="PLM90_11375"
     CDS             371..667
                     /locus_tag="PLM90_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90023.1"
                     /translation="MANNTSSHILNTSANLLGFCLFVITSLHIADYSETHLIDELTSA
                     IALLLTISCICSFISIRTKDSRREKYLESIADYFFMGSLIGIFIIILLITINFI"
     gene            671..1438
                     /locus_tag="PLM90_11380"
     CDS             671..1438
                     /locus_tag="PLM90_11380"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90024.1"
                     /translation="MALSTKAGNKVAEITLIFWLMKIVATTLGETLGDFISMTSNLGY
                     SMGILITMLFFVIMLLIQLRLNKYVPVVYWLVIIGTTTLGTEISDYIDRSLHLGYTAG
                     SVLLISGLLGTLFLWNRKFNNLSVYPIYERQKEFYYWTAVLFSNSLGTAFGDFLGEHF
                     GLSYLQGALVTGAVILIVVLLHYRSQINHIVLFWIAFVFTRPFGATFGDFLTKPIEKG
                     GLQLGTLNSTLVATGVMIILLFIATNRHKQGEERLDN"
     gene            1837..2247
                     /locus_tag="PLM90_11385"
     CDS             1837..2247
                     /locus_tag="PLM90_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HMZ90025.1"
                     /translation="MQYWLIKSEPEAYSWDDLVEKKIDIWSGVRNYAARLHLRAMKKG
                     DLCLFYHSGNVSACVGIAKVVKAAYPDPTAEEGDWSAVDVAPVKKLKREVSLKEIKLI
                     PALADMPLVRISRLSVSPVTEFQYHKILDMSNDK"
     gene            complement(2312..3481)
                     /locus_tag="PLM90_11390"
     CDS             complement(2312..3481)
                     /locus_tag="PLM90_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ90026.1"
                     /translation="MQFYERKYRLKLILVLVAMVIVAATLLYTNNLATKLAEEERQKV
                     TQIADVYHYIATANDNTDFRFFIELIQANKTVPIISTDNDDNIVAFLNIDSAKVAKDT
                     TYLKKRLEEMKDFAEPIKLELSAGIYQYVYYQHSDLYQQLLYYPYVQLMIIAAFLIVA
                     YTLFNIARRSEQNRVWVGMAKETAHQLGTPISSLAAWVEYLENIPELDGRKHVMEEMD
                     KDVQRLVLIADRFSKVGSQPDLQPCHLGDELARSIDYIKRRSSQKAVFNFEQSDSDII
                     VNMNVILFNWVIENLLKNALDAMEGVGEITVEIRTENDQAIIDISDTGKGIPRSKQKT
                     VFEPGYSTKKRGWGLGLTLAKRIVESYHKGKIFVKTSIPGKGTTFRIQLPLSNTK"
     gene            complement(3536..6169)
                     /locus_tag="PLM90_11395"
     CDS             complement(3536..6169)
                     /locus_tag="PLM90_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HMZ90027.1"
                     /translation="MQETNPSAVPHQDTPKPEKPRRKKILKYIFVGFGIFIGLILLIA
                     ILVPVLFEDKIKALFVAELNKSLATPVTINEDDIHLSLFQHFPEASLIFNNVGIRESF
                     PKSQKNFLEAKEISLVFNFWNILKSNYTIKNIVVKDGYCRLITDKRGNINYKFWKESD
                     DTTSSDFQIQLDRVECMQMDMQYIDYKYGQDVDLQLHDCTLSGNFSSDLYTMTATGDL
                     LSRRIRVGSANYLVNKEAHVDTKLLVNVPEESYTFEGGKITVEENTFLLDGSIALKGD
                     DMYDLTINGDEIDLAGLMLLLPGNISGNLESLETKGDINFAATIKGPYTATKDPAINI
                     DFSVRDGSIKHEKFGGRLDNMAFTGKYTNGSKQQSATSSISIKDFSASRDGNPLEMAL
                     VYSNFVNPFIDLQLNGELPASLIIPLALPDVKDADGMIKLNNINIKGNIKSFNADQGQ
                     APPTGTITFEGVSCTMNGEHVEIPAGVAVVSNNELSLQQIKLSAAGSNLLADLTIGNW
                     IQHVFPSAISPALNINGSIQAENFDVTGLLTLLGNESDTEVTTAPTTATATSTSTTSD
                     PMNISGQVDLKCGRLTYDQLSLEDVKTTVKLSPGFIVLNDMSGNTMNGKFIMNTSFRT
                     LPTGELLLQTSGQLIAIDVRELFRQLDNFDQSYLTDKNLKGSITGNIYDVAVKWNADY
                     SLDEQSIYALCDMRIENGELIEYKALESLSAFVKLSDLRHIKFSTLENKIEIANRQVK
                     LPAMQIKSNAMDLYMSGTHSFDNVIDYQFRISLADLMVRKFLGGNKQQDNYETDAEGG
                     VNVYVSMTGTVDDPILKYNKREAKQKLQDSGLEQQRFIDIFKRDPEETMFHETETPEV
                     KPGETDTEDIEFIEFDEEEDK"
     gene            complement(6286..8127)
                     /locus_tag="PLM90_11400"
     CDS             complement(6286..8127)
                     /locus_tag="PLM90_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ90028.1"
                     /translation="MPKLLLLPALLWSLALSAQYNLVPNPSFEIYNTCPVTISGFDYY
                     SDTYVDDWYSCGGGTSDYFNACAGAGSWVGVPENLFSLDQPAHTGVGYGGFWVDLYDD
                     NSFIYREYCQAELIEPLVAGQCYYVEFWSAPATLSDFFGANHGTTDAIGAFLGTEKIG
                     DPGDYGVLAETPQIDNNGTGNYIDPPALWTKVSGFFEAAGGESWIAVGNFHPDDEVEM
                     VPYTGGPTDVSLVYLFVDDVLVSPVDSMTYLPDTISCNPVTLYAPEGADSYLWSTGAT
                     TSSITVFETGEYWVQINLPCTTVSDTALVQIVADSVYTTESVIQICFTELPYTLAADP
                     TYEGYLWNTGETTSSIEITDGGVYVVNGFADCATFVDSFSVEVIPPMADIPNLQDTLI
                     CQSTWSLELNGPDGYDAYNWSTGATTQNITVSDAGTYSLTVESTCETLSDEMTITEDA
                     YLLSDIDLGQDQYLCPPAGITEITIASDITLPDYQWNTGAETQAITVTEPGTYFVSYE
                     SLCKTLSDTIEITECIGLGIPTAFSPNNDGTNDQFMVVTLHPEDILSFNIYNRWGQLL
                     FSGDAAHYAWDGTFNNTPQPMGSYVYVLHFLNDGVEEVLQGNFTLVR"
BASE COUNT         2326 a         1703 c         1771 g         2507 t
ORIGIN      
        1 acgccgctaa atcaaacaac agcgacgctg aagaaaacac tgaagcctga taatcgtttt
       61 tcaacaaaag ccctgccctc aaaagcaggg ttttttgttt taggtagcaa ggggggctac
      121 attgttcgcg ctaagctgct aagttgcaaa ggcgctaagt gcacaatact acggaccgca
      181 ttcaggttga tagtatctag acttggtatc aggtatcaag tatcaggtat caggtaaaaa
      241 ttcacgattc acgattcacc cactcaccca ctcacccact cacccactca ccactcacca
      301 cccgcctgct cggcgaggca agctcagccc tctcttcact aactttgcgt cattaactca
      361 aaacttaaaa atggcaaata atacttcttc tcacattctc aatacctccg caaatctatt
      421 ggggttttgc ttgttcgtga tcacttcttt gcatattgcg gattattccg aaacacatct
      481 gattgatgag cttacaagtg ctatagcttt attgctgact atttcatgta tttgctcgtt
      541 tatttccatt cgaacaaaag atagcagacg agagaaatat ctggaatcaa ttgccgatta
      601 tttctttatg ggctccttga tagggatatt tatcattatt ttgctgatta ccataaattt
      661 tatttaagat atggctttgt caactaaagc cggaaataag gtagcggaga taacgcttat
      721 tttttggctc atgaaaattg tcgccaccac gcttggcgaa acccttggtg attttatatc
      781 gatgacctca aatcttggtt attcgatggg catattgatt acgatgctgt tttttgtcat
      841 tatgctgtta attcaattaa ggttgaataa atatgtacct gttgtctatt ggttggtgat
      901 tattggaact actacattag gtactgaaat ttcggattat atagacaggt cgctgcacct
      961 cggttatacc gcaggcagtg tgctgttgat tagtggatta ctgggaacgc tttttctgtg
     1021 gaacaggaag tttaataatc tttcagtgta tccgatttat gaaagacaaa aggagttcta
     1081 ttattggact gccgtacttt tttcaaacag tctgggtact gctttcggtg attttttggg
     1141 tgaacatttc ggattaagtt atttacaggg cgcattggta actggtgctg tgattttaat
     1201 tgtggtgttg ttgcactata gatcgcaaat caatcatatt gtcttatttt ggatagcatt
     1261 tgtttttaca cgtccatttg gtgcaacctt cggcgatttc ttaaccaaac cgatagaaaa
     1321 aggcggactg caacttggaa cgctcaattc aactttagtg gcaacaggtg tgatgataat
     1381 tttattattt attgccacca atcggcataa gcagggtgag gaaaggcttg ataattaaga
     1441 ttcagattag ttgttttcgt tgagcaaatc aggggggaaa aaaaggagaa aggaaaaagg
     1501 agagaggaaa aaggagagag gagagtggat gagcttgcct cgccgagcag gcgggtgggt
     1561 gagtgataag caaattcgct tgcaaagcga gcgaaaaatt gcattagtct cactactcaa
     1621 tacccactac tcactaccag tcgtgaatcg tgattcgtga ctcgtgagtg tggtgcgtac
     1681 ataaggatca gagatgtgat tcgagtatgc atttacctga tacctgatac ttgatacctg
     1741 atacctgaaa cctgatacaa attctaacac ccgcctggag cgaagcaagc ttgactcccg
     1801 gtactcaatt ctcccactcc atccttatat ttgcatatgc aatactggtt aataaaatca
     1861 gaacccgaag cttattcgtg ggatgatctg gtagagaaga aaattgatat ttggagtggt
     1921 gtacgcaatt atgctgcacg attgcatttg cgtgccatga aaaaaggtga tttgtgtttg
     1981 ttttatcata gcggaaatgt ttctgcctgc gtaggaattg cgaaggttgt taaagcagca
     2041 taccctgacc ctactgctga agagggcgac tggagtgccg tagatgttgc tccggtaaaa
     2101 aagttgaaac gtgaagttag tttgaaagaa attaaattga ttcctgcact tgcagatatg
     2161 cccttggtgc gaattagcag attgtctgta tcaccggtta ctgaatttca atatcataag
     2221 atattggata tgtcgaatga taaatgaaga taatgtttta gcagtttgcc gtaatccgga
     2281 agatcaaaac gctcaaacct ttcattaatt tttacttcgt attactgagt ggcaattgta
     2341 tccgaaaggt tgtgcctttt ccgggaatag acgtttttac aaatattttt cctttgtgat
     2401 agctttcaac aatgcgtttt gccagtgtga gtcctaatcc ccacccacgt ttttttgtag
     2461 agtatccagg ttcaaaaaca gttttttgtt ttgagcgtgg aatgcctttg ccggtatcgg
     2521 agatatcaat aattgcttga tcattttctg tgcgtatctc aacagttatc tcgccaacac
     2581 cttccattgc atcaagtgca tttttaagta gattttcaat cacccagtta aacagaatca
     2641 cgttcatgtt tacgatgatg tcactatcag actgttcgaa attgaataca gctttctgtg
     2701 atgagcgacg tttgatgtaa tcaatagaac gtgctaattc atcgccaagg tgacagggtt
     2761 gcagatcggg ctgcgaacct acttttgaaa aacgatctgc aatcaatacg agtcgctgca
     2821 cgtctttgtc catttcttcc atcacatgct tgcgaccatc tagctcagga atattttcca
     2881 ggtattctac ccaagctgcc agagaactga ttggtgttcc taattgatgt gcagtctctt
     2941 tggccattcc cacccatact ctgttttgct ccgaacgccg tgcaatattg aaaagggtat
     3001 atgctacaat taagaatgcg gcaatgatca ttaattgcac gtacggataa tataataatt
     3061 gctggtagag atcggaatgc tgataataca cgtattgata gatgccggca cttaattcca
     3121 atttgatcgg ttcggcaaag tccttcattt cttcaagacg ctttttcaaa tacgtagtat
     3181 ccttggcaac tttagcggaa tctatattca gaaaggctac aatattatcg tcattgtcgg
     3241 tagaaataat gggaactgtt ttattggctt ggatcaactc aataaaaaat ctaaaatccg
     3301 tattatcatt cgctgtggcg atatagtgat atacgtcggc tatttgcgta accttttgcc
     3361 gttcttcttc tgcgagcttg gtagcgagat tgtttgtata cagcagagtg gcagctacaa
     3421 taaccatagc caccaatacg agtattagtt tcagtcgata cttacgttcg taaaattgca
     3481 tgctcacggt atcggctaag ttaatgggtt tctgataaat tgttgaacag cactgctatt
     3541 tgtcttcctc ttcgtcaaat tctatgaatt ctatatcctc ggtatctgtt tcgccaggtt
     3601 tcacctcggg tgtttcagtt tcatgaaaca ttgtttcttc aggatcgcgc ttgaaaatat
     3661 cgataaagcg ctgctgctcg aggccactat cctgaagttt ttgtttcgcc tcacgcttgt
     3721 tgtatttcaa tatcggatcg tcaacggtac ctgtcatact cacatacaca ttcacgcctc
     3781 cttcagcatc agtttcgtaa ttgtcctgct gtttatttcc accaagaaat ttgcgcacca
     3841 ttaaatcggc gagactgatt cgaaattgat aatcaattac attgtcgaaa ctgtgtgtgc
     3901 cactcatgta taagtccatc gcattggatt tgatctgcat ggcaggaagt ttcacctgac
     3961 gattcgcgat ttcaatttta ttttcaagag tggagaattt aatatggcga agatcactca
     4021 attttacgaa tgcagagagc gactccaagg ctttatattc tatcaactct ccattttcaa
     4081 tacgcatatc gcacaaagca taaatagatt gctcatcaag cgagtaatcg gcattccact
     4141 ttactgctac atcataaata tttccagtaa tgctaccctt aagattttta tctgtcaaat
     4201 acgactgatc aaaattatcc aactgtcgga ataattcacg cacatcgata gcaattaact
     4261 gcccggaagt ttgcaataat agttcacctg ttggcaatgt tctgaaactc gtattcatga
     4321 taaatttgcc attcatggta tttcccgaca tgtcattcaa cacgataaat ccgggagata
     4381 atttcacagt ggtttttaca tcctcaagac ttagttgatc ataagtcaaa cgtccgcatt
     4441 tcaaatcaac ttgaccgctg atattcatgg ggtcggaagt agttgaagta gatgttgctg
     4501 tagctgttgt gggagcagta gtaacctctg tatcactttc gttacctaat aaagtcagta
     4561 agcctgtaac atcaaaattt tccgcttgaa ttgaaccatt gatatttaag gcaggcgata
     4621 ttgctgatgg gaaaacatgt tgaatccaat tgccaatagt aagatcggca agaagattac
     4681 tcccggccgc agaaagtttt atttgttgaa gtgaaagctc attatttgaa actactgcca
     4741 cacctgcagg aatttctaca tgttcgccat tcatagtaca actcacgcct tcaaatgtaa
     4801 tggtgcctgt tggaggtgcc tgaccttgat cggcattaaa ggatttgata tttcctttga
     4861 tgttgatatt attcaattta atcatgccat cagcatcttt tacatcaggc aaggctaatg
     4921 gaataattag tgaggcaggc aattcgccat ttaattgaag gtcgatgaat ggattcacga
     4981 aattggaata taccaatgcc atttcaagcg gattgccatc gcgcgaagcg ctaaaatctt
     5041 ttatgctgat ggagctcgtg gcactttgtt gtttgctgcc attggtgtac ttaccggtaa
     5101 atgccatatt gtcgagccta ccgccaaatt tctcatgttt gatacttccg tcgcgaacac
     5161 tgaagtcaat atttatggca gggtcttttg tggcagtgta aggccccttg atggttgcag
     5221 caaagttgat atcacctttt gtctccagag attcgagatt gcctgatata tttccgggaa
     5281 gcaacagcat caatccggca agatcaattt catcgccgtt aatagtgagg tcatacatgt
     5341 cgtcgccttt aagagcaatg ctgccatcga gtaaaaatgt attttcttca acggtaattt
     5401 taccaccttc aaaagtgtaa ctttcctccg gcacgtttac taataatttg gtatcgacat
     5461 gtgcttcttt gtttacaaga taatttgcac ttccaactct gatacgtcgg ctcaataaat
     5521 ctcccgtggc cgtcatggta tatagatccg aagaaaagtt tccggaaagt gtgcaatcgt
     5581 gcaattgtaa atctacatcc tgaccatatt tgtaatcaat gtattgcata tccatctgca
     5641 tgcattcaac acgatctaat tgaatttgaa aatctgatga agtggtatcg tcagattctt
     5701 tccagaattt gtagttgatg tttccgcgtt tatcggtgat caatcggcaa taaccatcct
     5761 taactactat attcttaatg gtataattac ttttcagaat attccagaaa ttaaatacca
     5821 ggctgatttc tttcgcttca agaaaattct tttgtgattt cggaaagctt tcgcgtatgc
     5881 ctacattatt aaaaatcaat gaagcttcgg gaaaatgttg aaataatgaa aggtgaatat
     5941 catcttcatt aatagtaacc ggagttgcca gacttttatt taactcggca acgaataagg
     6001 cttttatctt atcctcaaac agaacaggca ccagaatggc gataagcagt atcaacccaa
     6061 taaaaatgcc gaagccgaca aaaatgtatt ttaggatctt tttgcggcgc ggtttttccg
     6121 gtttcggtgt atcctgatgg gggacggctg atggattcgt ttcctgcacg gtcaaagtta
     6181 aggatgtatg ggataacgaa gtttacgcaa cgaatagttt tatggttgcg aaaaattaac
     6241 caaacatgcc tcgtagtcga gaccctcggg ggaataatat tccgattatc gcaccaaggt
     6301 gaaattacct tgtaacactt cctcaacgcc atcattaaga aaatgcagca catacacata
     6361 actgcccatc ggttgtgggg tattgttgaa ggtgccatcc catgcgtaat gggctgcgtc
     6421 gccactaaac aataactgtc cccatcgatt ataaatgttg aaggaaagga tgtcttcagg
     6481 atgcagtgtc accaccataa actggtcgtt cgtgccatca ttgttaggac tgaaggcagt
     6541 tggtattccg aggccaatac attcggttat ctcaatggta tcgctcagtg tcttacataa
     6601 actttcatac gatacgaaat aggttcccgg ttcagttaca gtgatagctt gggtttcagc
     6661 gccggtattc cattgataat ccggaagggt aatatctgat gcaatggtga tttccgtaat
     6721 gccggccggt gggcacaaat attgatcctg accgaggtct atatcgctga gcaggtaagc
     6781 atcttcggtg atggtcattt catcactcaa tgtttcacag gtgctttcaa ccgtcagtga
     6841 atatgtgccg gcatctgata cagtaatatt ttgtgttgtg gcgccagtgc tccagttata
     6901 ggcatcgtag ccgtccggtc cattgagttc taaactccag gtagattgac aaataagtgt
     6961 atcctgtaga tttgggatgt cggccattgg aggtattact tcaacactga aagaatcaac
     7021 aaatgtggca caatctgcaa aaccattgac aacatagacg ccaccatcgg tgatctctat
     7081 gctggaggtc gtttcccctg tgttccagag atacccctca taagtagggt ctgcagccag
     7141 cgtgtaaggc aattctgtga agcaaatttg gattacactc tcagtagtat agaccgagtc
     7201 ggcaacaatt tggactagag cggtatctga gacggtagtg caaggtaaat tgatttgtac
     7261 ccagtattca ccggtttcga aaactgtgat ggacgaagta gttgctccgg tgctccacag
     7321 gtaactgtcg gcgccctccg gggcatacaa tgtaacggga ttgcagctga tagtatcagg
     7381 caagtaggtc atagaatcta caggcgatac cagcacatca tctacaaaca aataaaccaa
     7441 agaaacatct gtaggtccac cggtataagg gaccatttcc acctcatcgt cggggtggaa
     7501 atttccgaca gcaatccagc tttcgccgcc tgctgcttca aagaatccgg atactttcgt
     7561 ccaaagagca ggagggtcaa tatagtttcc tgttccgtta ttatcgattt gaggggtttc
     7621 tgcgaggaca ccatagtcgc ccggatctcc gattttttct gttcccagaa aggcacctat
     7681 ggcatcagta gtgccatgat tagcgccaaa gaagtcggac agggtagcag gggcactcca
     7741 gaattcgaca taatagcatt gtcctgccac gagcggttct ataagctcag cctggcagta
     7801 ttcacggtat ataaaggagt tatcgtcata cagatcgacc caaaatccgc catacccaac
     7861 gccggtatga gccggttgat cgagagaaaa caagttttca ggcacaccca cccacgagcc
     7921 ggcacctgcg caggcattga aataatcgga agtcccgccg ccgcaagagt accagtcatc
     7981 cacatatgtg tcgctgtagt agtcgaaccc cgaaatagta acagggcatg tattataaat
     8041 ttcgaagcta gggttgggca ccaggttata ctgtgccgac aatgccagcg accataataa
     8101 tgccggaagg aggaggagtt taggcatgtt caaatatatc aaatttgaca atgaaacatg
     8161 tcttccaaat gtaaagccac gaaaacacat agaaaaaatg ctttcctgtt tggaaaattt
     8221 gctctgagtg ttatctttgc actctctttt tggggagcat agctcagttg gttcagagca
     8281 tcccgcttta atgcgggagg gtccagg
//
LOCUS       DAOQOU010000145         8267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4762_length_8267_cov_5.694837, whole genome shotgun sequence.
ACCESSION   DAOQOU010000145 DAOQOU010000000
VERSION     DAOQOU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_8267_cov_5.694837"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PLM90_11405"
     CDS             <1..1393
                     /locus_tag="PLM90_11405"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HMZ90029.1"
                     /translation="LFLHPTEGAFAEVYLVAGANALRPAAGNNGFTAGLDVSILVIDA
                     AKGNSEGSNVLNYDKFRLNTPVFQDTNLVDFSIIDQKRLAIPNKTVTVEVTVTDINDP
                     SNYFVSTEVLTSYQENMIQISDIQFAESYKPVDQENNFSKNGVELMPYPLTFFPSTKS
                     SIILYGEVYGADKYLQDDQFLVAFSIKNASTNEFNPTFYQYVKAEKQPVFSFLREINI
                     ADLPSGNYNLYVEVRNKKNELITQKVVYIQRANTGPVYAWENIKMINTAGTFTEEYSE
                     EQLDYFLDVIRPRATESDLNLIESLSGRVEPDMKRKFLYNFWVERNAADPYGEWKKYL
                     ALVKEANNSFGTPSRQGYKTERGRVYLQYGPPYDVVTSVNEAGAYPYEIWFYTTLPDK
                     QTNIGFAFYEPTMVSNDYILLHSNARGELHEPRWKVKLYENVASPQEVLNFDNTEVED
                     KIGGHRAVDMYQF"
     gene            1456..2517
                     /locus_tag="PLM90_11410"
     CDS             1456..2517
                     /locus_tag="PLM90_11410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ90030.1"
                     /translation="MQADDILQRPAVAIVILTWNGRKFLEQYLPSVVRLPYQPLDIYI
                     ADNASTDDTVDFVKANYPSIKIIAIPKNEGFAKGYNVALKQVVADYYFLVNQDVELTP
                     NCLEPMIEIMESNVMIAACQPKILAAWDKSRFEHAGAAGGYIDAFGYPFCRGRLFNIE
                     EQDTGQYADAREIFWASGAALLIRAELFNRFKGFDPDFFAHMEEIDLCWRLKRAGYQI
                     HYIPEATVYHVGGGSLPKENPYKIYLNFRNNLSMLFKNYETTDLIWRFPVRILLDNAA
                     FFHALFNGRRKEAGAIFRADWHFVIALPLHMKKRFDTWRQYHRNRVAVSRVKMSGYYN
                     KSIVWQFFARGKKYFSELP"
     gene            complement(2514..2933)
                     /locus_tag="PLM90_11415"
     CDS             complement(2514..2933)
                     /locus_tag="PLM90_11415"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HMZ90031.1"
                     /translation="MRILILNGPNLNLLGVREPEIYGTESFETFFAKLQQRFPNVELI
                     TMQSNHEGALIDALHAYGFSYDGIIINAGGLSHTSIALADAISAITTPVVEVHISNTF
                     ARESFRHHDVLTANCKGIIAGLGLEGYALAVKYFLSH"
     gene            3017..3919
                     /gene="xerD"
                     /locus_tag="PLM90_11420"
     CDS             3017..3919
                     /gene="xerD"
                     /locus_tag="PLM90_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HMZ90032.1"
                     /translation="MNWNGAIKEFKGYLRLEKSLSPNSVEAYIRDITKLHQFLEGNFP
                     GTIPEKVDRQQLEGFLIFLHEVGMNDTSQARIISGIRAFYKFLLIEDLIDHDPAHLID
                     LPKLRRKLPDTLSYEEVMSIISAVDLSKKEGQRNKAMLETLYSCGLRVSELIGLRISD
                     MFTEDGFIRVRGKGDKERLVPIGDSAINYIKIYKETVRSHITPQKGFEDHVFLNNRGK
                     MLTRVYVFMLIKQLAEQAGIKKNISPHTFRHSFATHLIEGGADLRAVQEMLGHSSITT
                     TEIYTHIDREFLRSTLIQYHPRFK"
     gene            complement(4727..6856)
                     /locus_tag="PLM90_11425"
     CDS             complement(4727..6856)
                     /locus_tag="PLM90_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMZ90033.1"
                     /translation="MKTYSSEHIKNVVLVGHSGSGKTTLAECMLFEAGAINRRGTVSE
                     QNTTSDYNELEHERGNSVFSTVMHVDWKDTKINIIDTPGMDDFTGEVIAAFKVADTGL
                     LVLNAAHGVEVGTELIWEYTDHLKKPMILVVNHLDEDKADFENTVAQAKERFSRKVTV
                     VQYPYNQGLDFNAIIDVHTMKMYRFPADGGKPEKLPIPESEMEKAQRLHNELIETVAE
                     NDDTLMEKFFEKGTLEEEEMMKGMMLSLRHHDLFPVFCASAKHNMGSGRIMGFIDTCC
                     PSAAHMQPEKLTNGADLPCNSSNPTVAFVFKTVSEPHLGDMSFFKVCSGKVTSGMDLN
                     NTDTNSPERITQLFVLQGKKREQVNELLAGDIGATVKLKKTHTNNTLAIKGQDVQIVP
                     IHFPEPKVQAAIDVKNKGDEEKVGLALHHIHEEDPTVEIDHNMELKQTIINTQGELHL
                     QVLKWKIAHSYGVNIDFMEPRIPYRETIQKAVRADYRHKKQSGGAGQFAEVHMLVEPW
                     YEGMPDPPELNVRGREEHDLKWGGKLVFFNCIVGGAIDARFLPSILKGVMSKMENGPL
                     TGSYVRDVRVSVYDGKMHPVDSNDMAFKLAGMMAFRNAFREADPKILEPIYNLEVLVP
                     GDYMGEVIGDLQTRRGIVMGMDNDGHYQKIIARVPLAELYDYSSSLRSLTQGRGKFVR
                     SFAEYAAVPYEVQTELMASHKEEPAEA"
     gene            7284..7970
                     /locus_tag="PLM90_11430"
     CDS             7284..7970
                     /locus_tag="PLM90_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMZ90034.1"
                     /translation="MQVTYYGHSCFGVEVGGKHLLFDPFIRPNPLAAEIRVEDIKADF
                     ILLSHAHVDHTADALEIAKRTGAVIIGIWEIHSWAERNGVKNSHPMNIGGSFTFSFGT
                     VKMTSAVHSSSFADGSYGGSPAGFYIETAEGNFYYAGDTALHNDMKLLGKYANIDFAF
                     LPIGSNFTMGVDDALVAAKFIKCDQIIGMHFDTFGYIKINKSEAIAKFKERDRNLILM
                     EIGETIEVTQ"
     gene            8000..>8267
                     /locus_tag="PLM90_11435"
     CDS             8000..>8267
                     /locus_tag="PLM90_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ90035.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLAASLAVLEFKTLLVDADPQAN
                     STSGVGFDPKENRVNIYDCLMNDKHAKDAIVETETPNLHLLQSHL"
BASE COUNT         2423 a         1780 c         1694 g         2370 t
ORIGIN      
        1 ccttttcctg catcctaccg aaggtgcctt cgcagaagtg tacctggttg ccggagccaa
       61 tgccttgcgt cctgcagccg gaaataatgg gtttactgcg ggtcttgacg tatccatatt
      121 ggtgatagat gccgctaaag ggaattctga agggagtaat gtcttgaatt acgataaatt
      181 ccgactcaat acgcctgtat ttcaggatac caatctggtt gattttagca ttatcgatca
      241 gaaaagactg gcaattccaa ataagacagt taccgtagaa gttaccgtaa ccgatatcaa
      301 cgatccatct aattattttg tatcaacaga agtgctgaca tcctatcagg aaaacatgat
      361 acagatttca gatattcaat ttgccgaatc atataaaccg gtagatcagg aaaataattt
      421 ctcaaaaaat ggtgttgaat tgatgccata tcccttgaca tttttcccat ctacaaaatc
      481 aagcatcata ttatacggtg aagtgtatgg tgccgacaaa tatttgcagg atgatcagtt
      541 cctggtggca tttagtatca aaaatgcatc gactaatgaa ttcaatccta ccttctatca
      601 atatgtgaaa gctgaaaagc aacctgtttt ctcatttctg cgcgaaataa atattgcgga
      661 tctgccaagt ggtaattaca acttatatgt tgaggtccgc aataaaaaga atgaactcat
      721 cacacaaaaa gtagtgtata ttcaacgagc aaatacaggt ccggtttatg cttgggagaa
      781 tattaaaatg atcaataccg caggcacttt cacggaagaa tattctgagg agcaattaga
      841 ttattttctt gatgttattc gtccaagagc taccgaatca gatcttaatc ttattgaatc
      901 tctctccggt cgcgttgagc cggatatgaa aagaaaattt ttatataatt tttgggtgga
      961 aagaaatgct gctgatcctt atggtgaatg gaaaaaatac ctggcacttg ttaaagaagc
     1021 taacaacagt ttcggcacgc catcgcgaca aggatataaa actgagaggg gacgggttta
     1081 cctgcaatac ggacctccgt atgacgttgt tacttcggta aatgaagccg gcgcatatcc
     1141 ttatgaaatc tggttttata ccacacttcc cgacaaacaa acgaatattg gatttgcatt
     1201 ctatgagcca acgatggtga gcaacgacta tatcctcttg cattccaatg cacgcggaga
     1261 actgcatgaa ccacgctgga aggtcaagtt gtatgaaaat gtagcctccc ctcaagaggt
     1321 gttgaatttc gacaatactg aagtggagga caagattggc gggcatagag ccgtggatat
     1381 gtatcagttt taaagaattc cggacgtttt tcggcgtccc ttggcattcg ttccactttc
     1441 ctatctttac cctgcatgca ggcagacgat attttgcaaa gaccggcggt ggcgattgta
     1501 atactcacct ggaacgggcg taaattcctt gagcaatacc tgccatctgt agttcgtttg
     1561 ccatatcaac cacttgacat ttatattgcc gataatgctt ctaccgatga taccgttgat
     1621 tttgtaaaag caaattaccc ttcaattaaa atcattgcca tccccaaaaa tgaaggcttt
     1681 gcgaaaggat ataatgttgc attaaaacag gttgttgccg attattattt tttggtgaat
     1741 caggatgttg agcttacacc caactgcctc gagccaatga ttgaaataat ggaaagcaat
     1801 gtaatgattg cagcctgcca accaaagatt cttgcagcat gggataaatc gcgttttgaa
     1861 catgccggtg ctgcaggtgg ttatatcgat gcattcggtt atccgttttg cagaggaaga
     1921 ttatttaaca ttgaagaaca ggatacaggt cagtacgcag atgctcgtga aatattttgg
     1981 gctagtggcg cggcattatt gatacgtgca gaactattca ataggtttaa aggcttcgac
     2041 ccggattttt ttgcccatat ggaagaaatt gatctctgct ggcgattgaa acgcgcgggt
     2101 tatcaaattc actatatccc tgaagcaaca gtttatcatg ttggcggcgg aagtttgccg
     2161 aaagaaaatc cttataaaat atatctcaac ttcagaaata acctgagcat gttgtttaag
     2221 aactacgaaa caacagattt gatttggcga tttccggtaa gaatattatt agataatgct
     2281 gccttctttc atgcattgtt taatggccgc cggaaagaag caggcgcaat atttcgggcc
     2341 gattggcatt ttgttattgc actgccattg cacatgaaaa aacgttttga tacctggcgc
     2401 caataccatc gcaatcgcgt agcagtaagc agggtgaaaa tgtcgggata ttataataaa
     2461 agcatcgttt ggcagttttt tgccagagga aagaaatatt ttagcgagct gccttagtgc
     2521 gataaaaagt atttcactgc gagggcatat ccttcaagtc ccaagcctgc aataataccc
     2581 ttacaatttg ctgtgagcac atcgtggtgc ctgaatgatt ccctggcgaa agtgtttgaa
     2641 atatgcactt ctacaacagg agtggtgatg gcgctaatag catcggcaag tgcaatgctg
     2701 gtgtgcgata atccacctgc attgataata atgccatcgt aactaaaacc ataagcatgt
     2761 aatgcatcta tcagcgcacc ttcgtgattg ctttgcatgg tgatcaattc cacattcgga
     2821 aatcgctgtt gcaatttggc aaaaaaggtt tcgaaacttt cagtgccata aatctccggt
     2881 tcgcgaacac ctaacaggtt caaattcggg ccattgagga tgagaatacg cataactgga
     2941 caaatttacg attattcaca cagcaaaata gtatatcgga gatgcccgtt tttagtgtcg
     3001 aatgtaactt aacaccgtga actggaatgg cgccatcaaa gaattcaaag gttacctgcg
     3061 actcgaaaag tccttatcgc caaattctgt tgaggcttat atccgcgata tcactaagtt
     3121 gcaccagttt cttgaaggca attttcccgg aaccatacct gaaaaggttg atagacagca
     3181 attagagggt tttctcattt ttctgcatga ggtcggcatg aatgatactt cgcaggcaag
     3241 gatcatttcg gggattcggg ctttttacaa gtttctcctt atagaagacc ttattgatca
     3301 tgatcctgct cacctcatcg acctgccaaa acttcgacga aaattaccgg atacgcttag
     3361 ttacgaagaa gtgatgtcca ttatcagtgc agttgacctg agcaaaaaag aaggtcagcg
     3421 caataaggct atgcttgaaa ccttgtattc ctgcggactg cgtgtcagcg aattgatcgg
     3481 attacgcatt tccgatatgt ttaccgaaga tggttttatt cgcgttcgtg gcaagggcga
     3541 caaggagcga ctggtgccga taggggacag tgccatcaat tatatcaaaa tttacaagga
     3601 aactgttcga agtcatatca cccctcaaaa gggttttgaa gatcatgtgt ttctcaacaa
     3661 ccggggtaaa atgctcacca gagtatatgt tttcatgctc atcaaacagt tggcggagca
     3721 agctggcatt aaaaagaata tctcacccca tactttccgt cactctttcg ccacacacct
     3781 gatagaagga ggcgccgatt tgagggccgt tcaggaaatg ctcggacata gctctatcac
     3841 caccaccgag atatataccc atatcgacag agaattcctg cggtcgacat tgattcaata
     3901 tcacccaagg ttcaaataag attgccaaga ttttaactaa aaaacaggtt aatgggcaaa
     3961 aaaaaagcag agtgcacgga agaactccga ttcctctgct atccccatga aaacacaaaa
     4021 ctttatgaag atgcggtttt agccgccgtt caattcaaaa attgtaaata aataacgatt
     4081 aacagcacaa atatacaatc aatgcgctga ataatccaaa ttttttaaaa ccggcaactt
     4141 aatttaacaa tgtagttttc aaccggaaaa gcacattgta tcccttaaaa catgcaattg
     4201 agtttaaagt gtgcgattga ttatggtttt cagccgaaat gcaatcacgg caaatgtttc
     4261 ccggcaggtt ataaaattat tttctgttca aaaaaacctc tcgagtgtac ccggcttgat
     4321 ggaatacatg tatcctgaaa ccggtggagg ctagtacaca aatccgtctt taaagtaagg
     4381 ttccgggggt tttcgtcgaa ttatgttatc gtaacgaatc tcgggttgta tagcataaaa
     4441 atatatgtgt tgaccacgat caattcctta tgtactattt cacacaaact cacatgtttt
     4501 agcaaagcat ctttgcattg ttgctaacaa attgcaacat gcattattac accgctcacg
     4561 cctgagaacg gtgaataata tggccaatag tagtatgttt tgatgcttgt aatcagccga
     4621 ggtaaacacc tcggctgatt cgctttgtat aaatacaatg gtttacagca aaaaaaagca
     4681 acagtctcct caaactgttg ctcttattaa aatgatgctt ttataattat gcttctgcag
     4741 gttcctcctt atggcttgcc atcaattcgg tttgcacttc ataaggcact gctgcatatt
     4801 cggcaaaact gcgaacgaat tttcctcggc cctgagtaag ggaacgcagg ctactggaat
     4861 aatcatacaa ttcggccaaa ggaacacgag cgatgatctt ttgatagtga ccatcgttat
     4921 ccatgcccat tacaattccc ctgcgggttt gcaaatcgcc aatcacttca cccatataat
     4981 cgccgggcac taatacttcg agattgtaaa tcggttcaag gatttttgga tcagcttcgc
     5041 ggaaggcatt gcggaaagcc atcatacctg ccagtttaaa agccatgtca ttgctgtcga
     5101 ccgggtgcat ttttccatca tacacactta cacgtacatc gcgcacataa gaacctgtaa
     5161 gcggaccatt ctccattttg ctcatcaccc cttttaatat tgaaggcaga aagcgagcat
     5221 cgatggcacc accgacaatg caattgaaga aaacgagttt accaccccat ttcaaatcat
     5281 gttcttcgcg accacgcacg ttcaattccg gtgggtcagg catgccttca taccaaggct
     5341 caacaagcat gtgcacttcg gcaaactgac cggcgccgcc actttgtttt ttgtgtcgat
     5401 aatcggcgcg cactgctttt tgtattgttt cgcggtatgg aattcgcggt tccatgaaat
     5461 cgatgtttac accgtagcta tgcgctattt tccatttcaa cacttgcaag tggagttcgc
     5521 cctgagtatt aataatggtt tgcttgagtt ccatattgtg atcgatctct acagtaggat
     5581 cttcttcatg aatatgatgc agagcgagtc ccactttttc ttcatcacct ttatttttaa
     5641 catcaatggc agcttgcact ttaggttcag gaaaatgtat gggaacaatt tgaacatcct
     5701 gaccttttat tgccaaagta ttattggtgt gcgttttttt caattttacg gtggcaccaa
     5761 tatcacccgc caataattca ttcacttgtt cgcgcttttt gccctgcaat acaaataatt
     5821 gtgtgatgcg ttcaggacta ttggtatcag tattatttaa atccattcct gatgtcacct
     5881 tgccactgca tactttaaag aagctcatat cgcctaggtg aggctcactt acggttttga
     5941 atacaaatgc tactgtcgga ttagatgaat tacacggaag atctgcgccg ttggtaagtt
     6001 tttcaggctg catatgcgct gcggaagggc agcaggtatc gataaaaccc ataatgcgac
     6061 cggatcccat attgtgtttc gcagaagcgc agaataccgg aaacaggtcg tggtgacgga
     6121 gtgataacat catccctttc atcatttcct cttcttccag tgttcccttc tcgaaaaact
     6181 tctccatcag cgtatcatca ttttctgcaa ctgtttcaat caattcgttg tgcagacgct
     6241 gcgctttttc catttcagat tccggtatcg gcaatttttc cggtttaccg ccatctgcag
     6301 ggaagcgata catcttcatg gtatgcacat caatgatggc attgaagtct aatccctgat
     6361 tatatggata ttgcaccacg gtaacttttc tgctgaatcg ttccttggct tgtgcaacgg
     6421 tattttcaaa atcagctttg tcttcatcca ggtgattaac aacaaggatc atcggttttt
     6481 tcaaatggtc ggtgtattcc caaatcaact ctgttcctac ttctacgcca tgagcagcat
     6541 ttaacactaa taaaccggta tcggcaactt taaatgcggc tattacttct cccgtaaaat
     6601 cgtccatccc gggagtatca ataatattta ttttggtgtc tttccaatct acatgcatca
     6661 cagtgctgaa tacgctattc cctcgttcgt gttccagctc attataatca gaagtagtat
     6721 tttgttcaga tacagtacct ctgcggttaa tagctccggc ttcaaacaac atgcattcag
     6781 ccaacgttgt ctttccggag ccggaatgcc ctaccaatac aacatttttg atgtgttcac
     6841 tgctataagt tttcataacg tcctccttgg tttagacctg caatataaag aaaccgtaaa
     6901 acaatcggaa atgacataaa tcataagttc acctgcgaag aaatcaccta ttccttcaat
     6961 atgcatgcag gttttagccc gcagacacca aaacgcgcca ttttcaaccc cgaaacttta
     7021 acatttcaat tccggcaatc ctttcaaagc ctcaccgtga ctttcctcac cacccttagc
     7081 gccttcgcac cttaccgcct tggcgcgaaa aacatacatc atatacttaa actataaacc
     7141 actccttaaa ctctttttcc atacctcaaa acccactaaa acttatccac agagcgttta
     7201 gatgctgtta aaaagttatt gccttttggg tgggtaaggg cgaactaagc gcgtaatttt
     7261 acacccgttc agcacaaacg atcatgcaag taacttatta cggacattca tgctttggcg
     7321 tagaggtagg agggaagcat ctgcttttcg atcctttcat tcgtcccaac ccgcttgctg
     7381 ctgaaatacg tgttgaagat atcaaagccg atttcatcct attgtcgcat gcgcatgtcg
     7441 atcatactgc cgatgcactg gagattgcca agcgaacagg agcggtaatc atcggtattt
     7501 gggagataca ctcctgggct gaacgcaatg gggtaaaaaa ctcacatccg atgaatatcg
     7561 gcggtagttt cactttcagc ttcggcactg taaaaatgac ctctgcggtg catagcagct
     7621 catttgcaga tggtagttat ggtggcagcc cggcaggatt ttatatcgaa acggcagaag
     7681 gtaatttcta ttatgccggc gacacggcgt tgcacaacga tatgaagttg ctgggaaaat
     7741 atgccaatat cgactttgca tttcttccta ttggtagcaa ctttaccatg ggcgtggatg
     7801 atgcattggt agctgcgaaa tttatcaagt gcgatcagat tataggtatg cattttgata
     7861 ccttcggata tattaaaatc aacaaatcag aagctattgc gaaatttaaa gaacgcgacc
     7921 gcaatttgat tttgatggaa ataggcgaaa ctatagaagt aacacaataa cagttcatca
     7981 ttaaaaggaa acgaattaca tgggaaagat cattgccatt gccaaccaga aagggggagt
     8041 aggaaaaaca acaacagcaa tcaatctggc tgcaagtctg gctgtgctgg aatttaagac
     8101 attgcttgta gatgccgatc cacaagcaaa ttcaaccagc ggagttggct ttgatcccaa
     8161 agaaaatagg gtgaatatct acgactgtct gatgaacgac aaacatgcta aagatgctat
     8221 cgtagaaaca gaaacaccaa atttgcattt attgcaatca cacctcg
//
LOCUS       DAOQOU010000146         8267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4763_length_8267_cov_5.624330, whole genome shotgun sequence.
ACCESSION   DAOQOU010000146 DAOQOU010000000
VERSION     DAOQOU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4763_length_8267_cov_5.624330"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..688)
                     /locus_tag="PLM90_11440"
     CDS             complement(47..688)
                     /locus_tag="PLM90_11440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HMZ90036.1"
                     /translation="MPEILPEALERYVAEHSTPESDVLSALNRQTYLKVMMPQMLSGH
                     VQGRVLSMLSHMIKPKRILEIGTFTGYSALCLAEGLQEGGKLTTIDINEELTPMVKQA
                     IENAGMQNKIDVVVGNALQIIPTLNETFDIVFIDADKQNYQKYFDLVINKVPVGGWIL
                     ADNVLWSGRVIEQKHDKDTAAIHAFNQYVSTHPRVDQVIFAVRDGISVCRRVG"
     gene            828..3857
                     /locus_tag="PLM90_11445"
     CDS             828..3857
                     /locus_tag="PLM90_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ90037.1"
                     /translation="MYRHTILRLCICSIVLFHFSIAFGQVRTITGTTVDQQSGQPLPA
                     VSILIKNTQSGTVSDEYGKFSIEAKSGDTLTFNFIGYQPVLLAVSGTTNWTVSMQPEA
                     KMLSEVVVMAYGESSQRNQSGSVSKVSSKKLKEIPMASVDQILQGQVAGLQVNTYSGQ
                     PGGEINIHLRGVGSITAGSTPLFVVDGIPINSGVVGFGYNITWTSNGLAGINPNDIES
                     VSVLKDAAATAIYGSRAANGVIIISTKKGKAGKTKFELNSDYGFNTLAFNSNVRPLTA
                     DEFIDLTIIGMNNAGFGEVKQNNILEDLHAYDGVNTNWLDEVTQNGMQNSTNFSVRGG
                     DEKTKFYTSFGYFNQEGNTIDSKFNRLSGSLNMEHAASSRLKFKTNILLSRSHQNTPY
                     GSGYFRAPIISGYLLVPTYSPYDTTGGFNLNNNELATFNPLAIASMDTNYYNNKKIIG
                     GFGADYEIIKGLTLSTQLSLDQNYTDEFQYANPFYGDGVALDGWASSAYTDITNWVWT
                     NLAKYNRMLMSNRLAMDITLGHEAQSSKQNTIYAFGQSLPPNLDIMVLSAAATPGDVF
                     SNIYEYTFESFFANTQFKYNDKYLLSVSVRRDGSSRFGINNRYGIFYSVGGSWNIDQE
                     DFFKGGGMVSALRLRASYGVNGNSDIGNYDAQANYLYTGSYNGVPSSAPVSTGNYDLT
                     WEKNKPFNVGIDASFFKDRFDLKADYYTRTTTDLLLNVPVSQTSGFSTQLQNLGAMIN
                     KGIEIELGADILRHRSVSWHIEANFSTNKNEITELYNHQQFISGYNNVYTAALYLYRE
                     GESYGTFYIPLYAGVDAQTGDAMWYTDSTMTETTTDVAEAKNAIAGNAQPKFFGGLNN
                     SVSWKGITLSAQINYVTGNQIYDIWGGFFQSDGANPQYNQYNTLLDSWQQPGDETDVP
                     KYVYYNKSGSNSTSTRFLYDGDYVRLRDVTLSYDIPTNLATKLRMNSIRVYVRGTNLY
                     TWTKDKDLPFDPDVSYGYGDLNLPVMKSMIGGITLGF"
     gene            3884..5287
                     /locus_tag="PLM90_11450"
     CDS             3884..5287
                     /locus_tag="PLM90_11450"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ90038.1"
                     /translation="MKNLTSIIGICGVALITLLSSCKDEFLDKVPYDAIPFDDALLTE
                     DDMQAALNGVYSNMLGSGLYGRTVPIMGDLLADNVYISSANTGRYIPFNTYAIFDDNG
                     DVDGVWSSAYYAILNANQVISADLPANANVNQMRGEAYAARGMMYFDLVRIFAKPYNA
                     DNINSAGVPIVLTYQPEIKPARNTVGEVYAQAVSDLTTAISLMTEEKNSEYISKYVAV
                     GLLAKVYQYMGDWENAKTQSLDLINNGGYSLVDSADFVSFWADPGPSELSETLFELRM
                     DIAVNNGFNSLAAMYDDAVYGDALVDSNFYNSYSDADVRKQLMFEAELSGEPIVRVNK
                     YPNSSNTSDYDDIKIIRLAEVYLILAEAYYHLGDEGNALTYLNAIAQQREPSFEGYTS
                     SGETLLNDITNERRKELAFEGNRVFDLNRLGLDINRGPQFPSDALFIPISDYRRLMPI
                     PLYEINANPAMVQNPGY"
     gene            5440..6471
                     /locus_tag="PLM90_11455"
     CDS             5440..6471
                     /locus_tag="PLM90_11455"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMZ90039.1"
                     /translation="MRKLLLMVSLLWGLQPVGAQILPSYGDSRTATTGWQFLKIAPDG
                     RSAGMSEAFLAVVDDVSSLYWNAAGLTQLDSNRLHFGVQYTDYAAGTSMNFGGVSYRI
                     DDNTLIGVSMQYFDAGEMDVTTEFLPFGNGQTFRATDLGLGISLARELTDMFSLGITA
                     KYVREDLASVHNQNIVFDFGFQYDIGVQNTRFAVAITNFGFNTQPGGEIAVVTLSDSI
                     QATDFTEIAVPTVFRLGFAWDAIKNEKHILTTAFQLNHPTDNNETYSLGLEYSWKQLF
                     YARTGYTFATDRSALPAFGFGTYLHRNYGTIKFDYGFNYDAVLGMINKIGIAYTLPNG
                     IHKKSSATH"
     gene            6473..7696
                     /locus_tag="PLM90_11460"
     CDS             6473..7696
                     /locus_tag="PLM90_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90040.1"
                     /translation="MRRINIIILSAVIFTGLNSCDFLFGTREDAVVDEIFEEGAIDPE
                     LVQDEVGYVPVLPFWTDFIEPHDIFCGYDEMIYVVDAEGLKILDQTGTVFQTIAIPGA
                     TDVTQDRRLHTYVAGRVDYDVDGDGVMENLAAVYHLTGTSYGNVEIIDTLIHPFCDAS
                     RNITAFRGADDEAVEFTGLATLADNTLYVTRKGPRNDVSGIARADNAMLIYDENGNNT
                     SYANGLSPVTGNLKSVWDISGIATFAAPPQSLSGISGSAEFMICLDQPSAPYKGLWIT
                     QTVDPEAGIIYGENALLTVFDYTKADRFLYEPDRFIQPTDIYIAPDFTGYIFITDAGT
                     DSLYQFTRKGYEGVNPPAGSPNTKQIIASFGGVGSGPFNLIDPSGVAYLRKVVYVADK
                     GNDRICRYKLSSDLE"
     gene            complement(7735..>8267)
                     /locus_tag="PLM90_11465"
     CDS             complement(7735..>8267)
                     /locus_tag="PLM90_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HMZ90041.1"
                     /translation="KSLVDMINKEVAAGTLTAMIVRAADFYGPAVNTSVLIEMAAKPM
                     KAGKTANAMGNIDKIHTYTYTPDAGKATAVLGNTPDAYNQVWHLPTTKEKLTTRQWME
                     MAAKEIGVQPKIRVAPMWMLKLMGWFVPVMREFPEMMYQFDNDYIFDSTKFEKRFGIT
                     ATKPETGIKEMVAAMN"
BASE COUNT         2379 a         1701 c         1747 g         2440 t
ORIGIN      
        1 gactcacgat tcacgatgac gaaccccatc cacatgaaaa tccccatcaa cccactcgcc
       61 tgcaaacact aatcccatcc ctcaccgcga aaatcacctg gtctacccgg ggatgtgtgc
      121 ttacatattg gttgaatgca tgaatagccg cggtatcttt atcgtgcttc tgttcaatta
      181 cccttccact ccacaacaca ttatcggcaa gtatccatcc gccaacggga actttattta
      241 ttacgagatc aaaatatttt tggtaattct gtttatcggc atcaataaac actatatcaa
      301 atgtttcatt taatgtaggg attatctgca atgcattgcc taccaccaca tcgattttat
      361 tttgcatgcc ggcattttct attgcttgct ttaccatggg tgtaagttct tcattgatat
      421 cgatggttgt cagcttacca ccttcctgca atccctccgc aagacacagt gcactatatc
      481 cggtaaatgt gccgatctca agaatgcgct ttggttttat catgtgactg agcatactca
      541 acacccgacc ctgtacatga ccactcaaca tttgaggcat catgaccttt aggtaagttt
      601 gtcggttcaa tgcagaaagc acatcagatt caggtgtgct gtgctctgct acgtagcgtt
      661 ctaaagcttc cggaagtatt tctggcatag tacaaagtaa tagttcaaat gtcagatttt
      721 catcacacat tgcaagtatt atatatatgt gacaatgtat ttggcattat aagtatctta
      781 gtatcggtga ctcctctcac cggcatttaa gactaaacct aattactatg tacagacaca
      841 ccattttgcg tttgtgcatc tgcagcattg tgctatttca tttttcgata gccttcggtc
      901 aggtgcgcac gattacagga actactgttg atcagcaatc gggacagcca cttcccgctg
      961 tttccatcct cattaaaaac actcaaagcg gaaccgtttc agatgaatac ggaaagtttt
     1021 ccattgaagc taaatcgggc gacacgctga catttaactt catcggatat caacctgttt
     1081 tactagctgt ttcgggaaca acgaattgga cggtatccat gcaaccggag gcgaagatgc
     1141 tcagtgaggt tgttgtaatg gcctatggcg aatcatctca gcgcaatcaa tcaggatctg
     1201 tgagcaaagt gtcgtcaaaa aaattgaaag agatacccat ggcttctgtt gatcagattt
     1261 tgcaaggtca agttgccgga cttcaagtaa atacttatag cggtcagccc ggaggtgaaa
     1321 taaatattca cttgcgaggt gtaggctcca tcaccgcagg atcgacacca ttatttgtgg
     1381 tggatggcat tcccattaac agcggcgttg ttggcttcgg ctataatatt acatggacat
     1441 caaacggatt agcgggaata aatccgaatg acatagaaag tgtaagcgta ttaaaagatg
     1501 ccgcagctac cgcgatttac ggatcacgcg ctgcgaatgg tgtaatcatc atctctacaa
     1561 aaaaaggaaa agcaggtaaa acaaaattcg aattaaacag cgattatgga tttaatacac
     1621 ttgccttcaa tagcaacgtt cgaccactaa cggcagatga atttattgat ctcaccatta
     1681 tcggaatgaa caatgccggc tttggcgaag tgaaacagaa caatattctg gaagatttac
     1741 atgcctatga tggcgtaaat actaactggc tcgacgaagt gacacaaaat ggtatgcaga
     1801 attccaccaa cttctctgtg cgcggtggcg atgaaaaaac taaattctat acctcattcg
     1861 gttatttcaa tcaggaaggc aataccattg attccaaatt caatcgcttg tcgggttcat
     1921 taaatatgga acacgcagct tcatcgcgtt tgaaatttaa aaccaatatt cttttgtcgc
     1981 gttcacatca gaatacacct tatggcagcg ggtatttcag agcgcctatt atcagcggat
     2041 atttactggt acccacttat agtccgtatg atactactgg tggtttcaat cttaacaata
     2101 atgaacttgc cacatttaat cccttggcta tcgcatcaat ggatacgaat tattacaata
     2161 ataaaaagat cattggtggt ttcggggctg attatgaaat aataaaagga ttaaccctat
     2221 caacccaact cagtcttgat cagaattata cggatgaatt tcaatatgca aatccctttt
     2281 atggcgatgg tgttgcgctt gacggatggg cttcatctgc atataccgat attacaaatt
     2341 gggtatggac taaccttgca aaatacaatc gcatgctgat gtcaaacagg ctggctatgg
     2401 atatcacgct tggccacgaa gcgcaatcgt cgaaacaaaa taccatatat gcatttggtc
     2461 agagtctgcc tccaaacctc gacattatgg tgctttctgc ggcagcaact ccgggagatg
     2521 ttttcagcaa tatttatgaa tacacctttg aatcattttt tgcgaatacg caattcaaat
     2581 acaatgataa atatttgctg agtgtaagtg ttcgcagaga tggctcatcg cgttttggta
     2641 ttaataatcg ttatggcatt ttctattctg ttggcggttc atggaatatc gaccaggaag
     2701 atttctttaa aggcggagga atggtgagtg ctttgcgtct gcgcgcatcg tatggtgtta
     2761 atggcaattc cgatatcggc aattatgatg cacaggcaaa ttatttatat accggtagct
     2821 ataatggtgt tccttcaagc gcaccggtaa gcacaggaaa ctatgactta acatgggaaa
     2881 agaacaagcc tttcaatgtt ggtattgatg catcgttctt taaagatcgc tttgatttaa
     2941 aagccgatta ttacacccga acaactaccg atttattatt gaatgtacct gtatcacaaa
     3001 catcaggatt ctctacacag ctgcagaatc ttggcgctat gattaataaa gggatagaaa
     3061 tagaattggg tgcagatatt ctgaggcatc gttctgtaag ctggcatatt gaagctaatt
     3121 tctcaaccaa taaaaatgaa atcacggaat tgtataatca tcagcaattt atttccggtt
     3181 acaataatgt atatactgct gcattatatc tctaccgcga aggagaaagt tacggcacat
     3241 tctatattcc gctgtatgcc ggagtggatg cgcaaaccgg cgatgcgatg tggtatacag
     3301 attcaacaat gactgagaca actaccgatg ttgctgaagc taaaaatgca atagcaggaa
     3361 atgcgcaacc aaaatttttc ggtggattga ataacagtgt ttcatggaaa ggcatcaccc
     3421 tttcggcaca aataaattat gtcacaggca atcagatata tgatatctgg ggtggatttt
     3481 ttcagagtga tggcgccaat ccgcagtata accaatacaa cacactgctt gacagctggc
     3541 agcaacccgg cgacgagaca gatgtgccga agtatgttta ttacaataaa tcgggctcca
     3601 attccacatc aacacgattc ttatatgatg gcgattatgt gaggcttcgc gatgttactt
     3661 tatcctatga tattccgaca aacctcgcca ccaaattgcg catgaacagt attcgtgttt
     3721 atgttagagg cactaattta tatacgtgga caaaagacaa agatcttcca tttgatccgg
     3781 atgtgagtta tggatacggc gatcttaatt tgcctgttat gaaatcgatg atcggaggaa
     3841 taacacttgg attctaattg tcaacgagaa aaaatttact acaatgaaaa atctaacatc
     3901 tatcatagga atttgtggcg tagcactgat cactttgcta tcgtcatgta aagatgaatt
     3961 tttagacaaa gtgccatacg atgccattcc gtttgatgac gccctgctta cagaagatga
     4021 tatgcaggct gcgctgaatg gcgtttacag caatatgctt ggatcagggc tttatggcag
     4081 aaccgtgccg ataatgggcg atctgcttgc cgataatgtg tatatcagct ctgctaatac
     4141 gggcaggtat attccgttta atacttatgc catttttgat gacaatggcg atgtagacgg
     4201 cgtctggtca tcggcatact atgcaattct aaatgccaat caagtaatta gtgctgatct
     4261 acccgccaat gcaaatgtta accaaatgcg tggcgaggct tatgctgcgc gaggtatgat
     4321 gtattttgat ctggtacgca tttttgcaaa gccgtacaat gccgataata taaatagtgc
     4381 cggcgtgccg atcgtgctta cctatcaacc ggaaattaag cctgcgagaa atacggttgg
     4441 cgaagtatat gcacaagcag taagtgatct cactacggcc atcagtttaa tgacagaaga
     4501 aaaaaattct gaatacatca gtaaatatgt tgctgttggt ttgttggcaa aagtttatca
     4561 atacatggga gactgggaaa atgcaaagac gcagtcactt gatttgatta ataatggcgg
     4621 ttattcactg gtcgattctg ctgactttgt ttcgttctgg gcagatccag gaccatcaga
     4681 attatcggaa acacttttcg agttgcgcat ggatattgct gtcaacaatg gattcaactc
     4741 acttgccgct atgtatgatg atgctgttta tggcgatgcg cttgttgatt cgaatttcta
     4801 caatagttac tctgatgccg atgtacgcaa acaattaatg tttgaagctg aattaagcgg
     4861 tgaacccatt gttcgtgtaa ataaatatcc caattcttcc aatacttctg attatgacga
     4921 cataaaaatt attcgtctcg ctgaggtata tttaattctt gccgaggctt attatcattt
     4981 aggtgatgaa ggcaatgcgc taacatattt gaatgctata gcccaacaac gagaaccttc
     5041 atttgaggga tatacatcgt caggtgaaac gctgttgaat gatattacca atgaaaggag
     5101 gaaagaactc gcttttgaag gcaatcgcgt ttttgatctc aatcgcctgg gtttggatat
     5161 caatcgggga ccgcagtttc catcagatgc attatttatt ccgatttcag attataggag
     5221 attaatgccg attccattat atgaaatcaa tgcaaatccg gcaatggtgc aaaaccccgg
     5281 atattagtat gtagctcttt ccattttgtg taaaagcgaa acgcgtaatg tgtttcgctt
     5341 tttttatccc ttctgacaat tgaccacctc cgggctccaa aacaaacctg ttttaggcta
     5401 tatcggtctc cctaagcctt ttattatctt tgccccgcta tgcgcaagct attgttgatg
     5461 gtatcactgc tttggggcct ccaaccggtc ggggcacaga tattgccatc ctacggcgat
     5521 tcgcgcacgg ctactaccgg ctggcaattc ctcaaaatag ctccggacgg acgttcggcg
     5581 ggcatgagcg aagcttttct ggctgtggtg gatgatgttt catccctgta ctggaatgcc
     5641 gccggactca cccaactgga tagcaaccga ttgcattttg gcgttcagta taccgattat
     5701 gcagcgggaa caagcatgaa ttttggaggc gtaagctata ggattgatga caatacctta
     5761 ataggtgtga gtatgcagta tttcgatgcc ggtgaaatgg atgttaccac cgaatttttg
     5821 ccctttggca acggacagac atttcgcgca actgacttag ggcttggcat atcacttgca
     5881 agagagctaa ccgatatgtt cagcttaggt ataacagcaa agtatgtgcg tgaagacctt
     5941 gcctccgtgc acaatcagaa tattgttttt gattttggat ttcaatatga tatcggtgta
     6001 caaaatacac gctttgccgt agccattact aatttcggat ttaatacaca gccgggaggt
     6061 gaaatcgctg tcgttacact cagtgatagt atacaagcaa cagatttcac cgagattgcc
     6121 gtgccgacag ttttcagatt gggatttgcc tgggacgcaa ttaaaaatga aaagcatata
     6181 ctcactactg cctttcagct caatcaccca accgacaata acgaaactta tagtttaggt
     6241 ttggaatata gctggaagca attgttttat gcacgcaccg gatacacatt cgccacagat
     6301 cgcagcgcgt tgcccgcatt tggattcgga acatatttgc ataggaatta tggcactatt
     6361 aaattcgatt atggatttaa ttatgatgcc gtattgggca tgattaataa aataggaatt
     6421 gcttatacat tacctaacgg tatccataaa aaatcttcag ccacacatta atatgcgtcg
     6481 cattaatatt atcatattat cagcagtaat ttttaccggc ttaaacagtt gcgacttttt
     6541 attcggcaca cgagaagatg ctgtggtaga tgaaattttt gaggaaggag ctattgatcc
     6601 tgaattagta caagatgaag ttggctatgt tccggtatta cccttctgga cagattttat
     6661 tgagcctcat gatatttttt gcgggtatga tgaaatgatt tatgtagtag atgccgaagg
     6721 attaaaaata ctcgatcaaa ccggaaccgt atttcaaaca attgccatac cgggcgcaac
     6781 tgatgttaca caagacaggc gtttacatac ttatgttgcc ggtcgcgtag attatgatgt
     6841 tgatggcgat ggtgtgatgg aaaaccttgc agctgtttat catttaacag gaacgtcata
     6901 tggaaatgtt gaaatcattg ataccctgat acatccattt tgtgatgcat cgagaaacat
     6961 cacggcattc aggggtgcag acgacgaggc tgtggaattt acaggtttag ccacattggc
     7021 cgacaatact ttatatgtaa cacgcaaagg acctcgcaac gatgtgagtg gtattgcacg
     7081 tgcagataat gctatgctca tttacgatga aaatggcaat aataccagtt atgcaaatgg
     7141 attatcaccg gtaacaggta atttaaaaag cgtatgggat atttcaggaa ttgcgacttt
     7201 cgcagcacca ccgcaaagcc tttcaggaat ttcaggttcc gctgaattca tgatttgtct
     7261 tgatcagccg tcagcaccct ataaaggact ttggattaca caaaccgttg accctgaggc
     7321 aggcattatt tatggcgaaa atgcattgtt gacagtattt gattatacta aagccgacag
     7381 atttttatac gaacccgatc gttttattca gcccaccgat atttatatcg cacctgattt
     7441 tacgggctat atattcatta ccgatgcggg aacagattct ttatatcaat ttacgagaaa
     7501 agggtatgaa ggtgtaaatc cacctgcagg ctcaccaaat acgaagcaga ttattgccag
     7561 tttcggtggt gttggcagtg gtcctttcaa tcttattgac cccagtggag tagcctatct
     7621 ccgcaaagta gtttatgttg ccgacaaggg caatgatcgc atttgtcgct acaagttgag
     7681 cagtgattta gagtgatgct tatgtattaa cactactttc gttgtgacaa ataattaatt
     7741 catcgctgcc accatttctt taatgccagt ttcgggtttt gttgcagtga taccgaagcg
     7801 cttttcgaat ttcgtgctgt cgaaaatgta atcgttgtca aactgataca tcatctcagg
     7861 aaactcccgc attaccggta caaaccatcc cattaatttc agcatccaca ttggcgcaac
     7921 tctgattttt ggttgtacac caatttcctt tgccgccatt tccatccatt gacgcgtagt
     7981 gagtttctcc tttgttgtag gcaaatgcca cacctgatta tatgcatccg gcgtattgcc
     8041 taaaacagca gtggcttttc cggcatcagg ggtataagta taggtgtgaa tcttatcaat
     8101 attgcccatt gcatttgccg ttttgcccgc cttcatgggt tttgcagcca tttcaatgag
     8161 cacgctggtg ttaacagcag gcccataaaa atctgctgcg cgaactatca tagctgttaa
     8221 tgttccggct gccacctcct tattaatcat atctacaagc gattttc
//
LOCUS       DAOQOU010000147         8254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4774_length_8254_cov_6.089401, whole genome shotgun sequence.
ACCESSION   DAOQOU010000147 DAOQOU010000000
VERSION     DAOQOU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4774_length_8254_cov_6.089401"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PLM90_11470"
     CDS             <1..1279
                     /locus_tag="PLM90_11470"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HMZ90042.1"
                     /translation="LNPAMPITGKIPWDVNFVAFGVNEDNNYIKISDPVFKYVTTSDS
                     ILDVEYYNPTQIRGHGNVLLQLPSAFIKVDDYAFGMFTNARSAGYFLSDKEPGGVIGL
                     NDLPLYTDLNMPAFNAGILVWSEIGLNAEMTLESNSLFSLHIGANIKYLMGLEALAFE
                     NNELFTFVKDTVNFTMTNFNTDFDYTRNLGSNLLYDPSNYALNGSGFGIDLGAYYVIH
                     KKSKFKYSKKLDYTWKFGAALLDFGAIKFQSNSGSYHLEEDQLFSVATLVLDSIDDID
                     EFNRTGSKTLYDNVDASKDASKFAMYLPASFLLTVDKPLRNDFYVQAMIMRRIPRFST
                     NLIARSNVFGLTPRYERRKFGFFLPMQLYEDREFRIGTAVRWHFVTVGSDNLRTWFTP
                     KEYSSIDFYVGVKLTPYWLVTQSKKKYLECLKN"
     gene            complement(1290..1964)
                     /gene="rsmI"
                     /locus_tag="PLM90_11475"
     CDS             complement(1290..1964)
                     /gene="rsmI"
                     /locus_tag="PLM90_11475"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HMZ90043.1"
                     /translation="MKGKLYIVPTPIGNLEDITFRAIRILKEADLILAEDTRTSSVLL
                     RHYGITTKAISHHKFNEHAQVQQLAQEIAEGKQIALISDAGTPAISDPGYLIIRTCLD
                     HALPVETLPGATAFVPALVNAGLPMHEFVFIGFLPQKKGRETKLRSLAAEQRTMILYE
                     APQRVVKLLEQLLQHLGDRRASVSRELSKKFEETQRGLLSELIKHFSEKEPKGEFVIV
                     VEGLQD"
     gene            complement(1982..2233)
                     /gene="purS"
                     /locus_tag="PLM90_11480"
     CDS             complement(1982..2233)
                     /gene="purS"
                     /locus_tag="PLM90_11480"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMZ90044.1"
                     /translation="MAYKAEINVMPLKELLDPKGKAVAGSMNNLSLTAIKDVRIGKRI
                     QLHIEASSASEAKRIAEEACKKLLANPIMESYEISISEG"
     gene            complement(2223..3011)
                     /locus_tag="PLM90_11485"
     CDS             complement(2223..3011)
                     /locus_tag="PLM90_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMZ90045.1"
                     /translation="MRLIQNLPNLMTLFNLFLGCMGVVYLYNDHMVIMSKQDDMLIDM
                     GLIHLTCYCVMLAGVVDFFDGFVARMLKVQSELGKQLDSLADMVTFGLVPGLVMYELL
                     ARAYYAHYNSFEYPILYYAAGFSVTLFAALRLAKFNIDSRQTTEFRGLPSPAMAMVVV
                     SLPLILLRNEMGMTSTLTNHWVLLGTIVLLSYLMVSDIPMLSLKVKNFKLQDNQWVFG
                     LLLIAAIILFFGLVIFHILFAVIPMIIFAYVILSITKNITENGI"
     gene            complement(3080..4375)
                     /locus_tag="PLM90_11490"
     CDS             complement(3080..4375)
                     /locus_tag="PLM90_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HMZ90046.1"
                     /translation="MKYLPIDSQLFVQNRKQYTSHLKPNSIAIFNSNDEMPFNADQTH
                     PWKQNSDIFYLSGIDQEETILLIYPDSPNPKYREALFLKKTNEHIAIWEGHKYTEAEA
                     RAASGIETIHWVEDFNTVFPMLMNHAEHVYLNTNENDRASIKVPTRDTRFIEETKLRF
                     PMHKYERSAPIMAKLRTVKSIWERDLMQIACDITNKAYRRVLNFVKPGVMEFEIEAEI
                     QHEFLRNRGTRPAYGSIIASGPSACVLHYVDNNLRCNDGDLLLMDFGVEYANYCADLT
                     RTIPVSGKFTQRQKDVYNAVLRVQKKAIDMMHIGMILKDFNTEVGKVMESELLGLGLI
                     SKQDIADQNPEWPAFKKYFMHGTGHFLGLDVHDIGDHYQPIPAGAVLTCEPGIYIREE
                     GIGIRIENDIMLMPDGSKYDFMKDTPSEVEEIETIMNSK"
     gene            4612..5535
                     /locus_tag="PLM90_11495"
     CDS             4612..5535
                     /locus_tag="PLM90_11495"
                     /inference="COORDINATES: protein motif:HMM:NF036911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HMZ90047.1"
                     /translation="MKLLKCLLPLSLLFLITQSCQKTDQLIPDAAIETSNDAFRLMTP
                     AYYRESENGSSIQVRFFESPRIFDLPKNSAEYANIKSLIDISRDAKQPMRVYFGDITM
                     PYISITGAQKASAEEIRLNEQKFVITPDNAKLKKVLPGMAVMNGIFDYCAAQGCTTGT
                     ATIDYCIPFQYVVDGCYARAHKMRQIMNEEYNYEPQKVFSYEGPSGSLAVDAGDCCVY
                     WWYHVAPLVTVKTATGTAQYVIDPSMFDEPVSIATWTAAQENLGCTPFADFGYTEITA
                     GKIYAPGGSTDNSYISTNWTLQFYADLVTCP"
     gene            complement(5609..6247)
                     /locus_tag="PLM90_11500"
     CDS             complement(5609..6247)
                     /locus_tag="PLM90_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ90048.1"
                     /translation="MKKILTIIFCSLAVAASAQQFRYGANVAPTVSFWLVEGDNYVPD
                     GMKVGFQVGGTIDLTLGDAERFALQSGLNFTSAPGAFKDNPEGLASNGKAWDYSITTL
                     DLPILLRLRSDELGKAVLFAQYGLTLGFTLTEKITVNDGKNGGSSFDYEGSNTSLSMG
                     AGAEFGLDNDKTLVFTGFFQNGIKSMVVNTNNPNNDSRYFPQQVGLRATMLF"
     gene            complement(6284..6949)
                     /locus_tag="PLM90_11505"
     CDS             complement(6284..6949)
                     /locus_tag="PLM90_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ90049.1"
                     /translation="MRKFTFLLLMLVAFSSRAQQFRYGFSIAPGSNKLVLPADMYDPA
                     VSFSGFQYGLLLDQTFVKGDIIGVSLGLNLNYSQSGMTSTDNNGAGDDKEWAVRARYL
                     EIPLTLKLRTPQFGSFTIYGEGGATYGKCLRAVGDYTLNGLTLDSDIDYLEKDNANGI
                     NYLDKNAGLQFGLGAEVEVAEGASIIIGFFYQKGIMNVYEDNNVGSDILLNQTGIRIA
                     GLF"
     gene            7525..7923
                     /gene="rpsL"
                     /locus_tag="PLM90_11510"
     CDS             7525..7923
                     /gene="rpsL"
                     /locus_tag="PLM90_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HMZ90050.1"
                     /translation="MPTIQQLVRKGRDVMKAPSKSRALDACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNQTEIIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGAL
                     DTAGVNNRKKSRSKYGTKRPKAGAAPAGKK"
     gene            7955..>8254
                     /locus_tag="PLM90_11515"
     CDS             7955..>8254
                     /locus_tag="PLM90_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HMZ90051.1"
                     /translation="MRKLKPKKRILPADPQFNDATVTRFVNMMMWQGKKSTAYTCFYD
                     ALALVEKQTGESGYEVWKKALSNVTPGVEVKSRRIGGANFQIPTEIRPDRKVSLSM"
BASE COUNT         2395 a         1775 c         1650 g         2434 t
ORIGIN      
        1 tttaaacccc gcgatgccga ttaccggcaa aattccatgg gatgtaaatt ttgttgcatt
       61 tggtgtcaac gaagacaata attatatcaa aatcagtgac ccggttttta aatatgtcac
      121 tacctctgat tctattttag atgttgaata ttataatccg acccaaattc gcggccatgg
      181 aaatgtttta ttgcaattgc cttctgcatt tataaaggtg gatgattatg cctttgggat
      241 gtttaccaac gctcgaagtg caggctattt tctgagcgat aaagaacccg gcggggttat
      301 tggcttaaat gacctcccac tgtatactga cctgaatatg cccgcattta atgcaggaat
      361 tttggtctgg agtgagattg gtctgaacgc tgaaatgact ttggaatcga attcgctatt
      421 ctctttgcat ataggcgcca atattaaata cctcatgggg cttgaagcgc tggcatttga
      481 aaataacgaa ctcttcacct ttgtgaaaga taccgttaat ttcaccatga caaattttaa
      541 tactgatttt gattatacca gaaatttagg ctccaatctt ttatacgatc cttcaaatta
      601 tgcattaaat ggcagcggat tcggaattga cctcggagca tattatgtca ttcataaaaa
      661 atcgaaattt aaatactcta aaaaacttga ttatacctgg aaattcggag ctgctttgct
      721 cgatttcggg gcgattaaat ttcaatcaaa ttcaggtagt tatcacctcg aagaagatca
      781 actattctca gttgcaacac tggtgctcga ttctattgat gacatagatg agtttaatcg
      841 caccggttcg aaaacattat atgataatgt ggatgcttct aaagatgcaa gcaaatttgc
      901 tatgtatctg ccggcatcat ttttacttac agttgataaa ccactgcgca acgattttta
      961 tgtgcaggcc atgatcatgc gccgtattcc cagattcagc acaaacctga ttgccagatc
     1021 gaatgtattc ggattgacac caagatatga aagacgaaaa ttcggattct tcctgcccat
     1081 gcaattatat gaagatcgcg aatttagaat aggaaccgca gtgcgatggc actttgtcac
     1141 tgttggatct gataatctgc gcacttggtt tacaccaaaa gaatatagct ccatcgattt
     1201 ttatgttggc gtaaaactca caccgtattg gctggttacg caaagtaaaa agaaatatct
     1261 cgagtgcttg aagaattgaa atcgcctgat tagtcttgta atccttccac aacaattaca
     1321 aattcacctt taggctcttt ctctgaaaaa tgttttatta attcgctgag caaaccgcgt
     1381 tgtgtttctt cgaatttttt tgaaagttct ctgcttacag aagcacgcct gtcgccaaga
     1441 tgttgtaata gttgttccag caattttaca acacgctgcg gcgcctcata taaaatcatg
     1501 gtgcgctgct ctgctgctaa ggaacgcaat tttgtttccc gacctttttt ctgtggcagg
     1561 aatccgataa atacaaattc atgcatcggt aatcctgcat tcaccaaagc cggaacaaac
     1621 gcggtcgctc cgggtaaagt ttcaaccggt aatgcatgat ctaagcaggt gcgtataatt
     1681 aaatatcccg gatccgaaat tgccggtgtg cccgcatcag aaattagcgc aatttgcttt
     1741 ccttcggcga tctcttgcgc caattgttgc acctgcgcat gctcattgaa cttgtgatgc
     1801 gaaatggctt ttgtcgttat tccgtaatga cgaagtaata cagatgatgt gcgcgtgtcc
     1861 tctgctaata tcagatccgc ttctttaagt atacgaattg cgcgaaaggt aatgtcttcc
     1921 aaattcccaa tgggagtcgg cacaatatat agcttacctt tcattgcaga tatatatcat
     1981 atcacccttc agatatcgaa atttcatagc tctccatgat aggattcgct aacaactttt
     2041 tgcaggcttc ttcagcaatg cgtttcgctt ctgaagcaga agatgcctct atatgcaatt
     2101 gaatacgttt gccgatgcgc acatctttaa ttgccgttaa tgataaatta ttcatgctgc
     2161 ccgcaacagc tttgcctttt ggatcaagta attctttcaa aggcattacg ttgatctctg
     2221 ctttatatgc cattttctgt aatattttta gtgatggaaa gtataacgta tgcaaatatg
     2281 atcatcggga tcactgcaaa aagaatatga aatatcacaa gcccgaaaaa taaaataatc
     2341 gctgcaatca atagtagtcc aaaaacccac tgattatcct gtaacttaaa atttttaacc
     2401 ttcaggctca acatcggaat gtcactcacc atgagataac ttaacaatac gattgtgcct
     2461 aataacaccc agtgatttgt tagcgttgat gtcatgccca tttcattgcg caacaggatc
     2521 aatggcaacg ataccacaac catggccata gcaggagagg ggaggcctct gaattctgta
     2581 gtttgcctgc tgtcgatatt gaattttgcc aatcgcaatg cggcaaatag tgttactgaa
     2641 aaccccgcag cgtaatataa aatgggatat tcaaacgaat tgtaatgtgc ataatacgct
     2701 cttgccaata attcatacat gaccaatccg ggcactaatc caaaggtgac catatcagca
     2761 agagaatcaa gttgttttcc tagttcagat tgcactttta acatgcgtgc gacaaagcca
     2821 tcaaaaaaat caacaacgcc tgccagcatc acgcaataac aagtaagatg tatcaacccc
     2881 atgtctatga gcatatcgtc ttgtttgctc atgatcacca tatgatcatt gtataaatac
     2941 accaccccca tacaacctaa aaacaggttg aacagtgtca tcaaatttgg aagattttgt
     3001 attaatcgca tatgatgtgt gatgtaaaca atgaaggttg attgattatt tgtatatggt
     3061 aataattatg ggcgattaat tatttactgt tcatgatagt ttcaatctct tcaacttcag
     3121 atggtgtgtc tttcatgaag tcatatttac tgccatcagg cattaacata atgtcatttt
     3181 ctatgcggat gccaatacct tcttcgcgaa tataaattcc cggttcacat gtcaatactg
     3241 ctcctgccgg aattggttga tagtgatcgc cgatgtcatg cacatctaaa ccaagaaaat
     3301 gtccggtgcc atgcataaag tactttttga aggccggcca ttccggattt tgatctgcaa
     3361 tatcctgttt gcttattaac cccaatccca acaattcgga ttccatgacc ttgccaacct
     3421 ctgtattgaa atctttcaat atcatgccga tatgcatcat atcaatcgct tttttctgca
     3481 cccgcaatac ggcattgtat acgtcttttt gacgctgtgt aaattttccg gaaacaggaa
     3541 tggtgcgcgt cagatccgca caataatttg catattcaac accaaaatcc attaacagta
     3601 aatcaccatc attacaacgt aaattgttgt ctacatagtg taatacacaa gcacttggtc
     3661 cgctggcaat gatacttccg tatgcgggtc tggtgcctct attgcgcaaa aattcgtgtt
     3721 gtatctctgc ttcaatctca aactccatca cacccggctt tacgaaattc aatacgcgac
     3781 gataagcttt attggtaatg tcgcatgcta tctgcattaa atcgcgctcc caaatacttt
     3841 taaccgtgcg caattttgcc atgataggcg cactgcgttc atatttatgc attggaaaac
     3901 gcaatttcgt ttcttcaata aatcgcgtat cgcgtgttgg cactttaatc gatgcacgat
     3961 cattttcatt tgtgtttaaa tacacatgct cggcatgatt catcagcata ggaaaaacgg
     4021 tattgaaatc ctcaacccaa tgaatggttt ctatcccact tgctgcgcgt gcctctgctt
     4081 cggtgtattt atgtccttcc catatggcaa tatgctcgtt ggtctttttg agaaataatg
     4141 cttctcggta ttttggattc ggactgtcgg gataaatcaa taatatcgtt tcctcctgat
     4201 caatgccact taagtaaaaa atatcggaat tttgtttcca gggatgtgtt tgatcagcat
     4261 tgaacggcat ctcatcattg ctgttgaaaa tggcaatgct gtttggcttt aaatgtgagg
     4321 tatattgctt gcggttctga acgaaaagct gactatcaat tggtaagtac ttcatgttgg
     4381 tgtctattac aggcttaaag gtagaaaaaa atgcccacca tgcccgtgac cccttgcctg
     4441 agcctagtgt caacctcgtc atattcaagg ctttgtatcc tgaaagttaa atattcctct
     4501 aatgcctacc agaactgcaa aaattgagca ggaagtcgta gttttataaa cctgtgtttt
     4561 gaataaacac gggtacgaca ttatataaat tcatcattaa aaccggttta catgaagctc
     4621 ctcaaatgct tacttccctt atccctcctt tttctgatca cgcagagttg ccagaagacc
     4681 gatcaactga tcccggatgc ggccattgaa acatcaaatg atgccttccg attgatgact
     4741 cccgcatatt accgcgaatc tgaaaatggc agttcaattc aagtcaggtt ttttgaatcg
     4801 ccacgcattt tcgatttgcc taaaaacagc gctgaatacg caaatattaa atcactcatc
     4861 gatatcagtc gcgacgccaa acagcctatg cgcgtttatt ttggtgatat cactatgcct
     4921 tatatcagca tcacaggtgc ccaaaaagca tctgccgaag aaattagatt aaatgaacag
     4981 aaatttgtca tcacccccga taatgctaaa ctcaagaaag tgttgccggg catggctgtg
     5041 atgaacggga tatttgacta ttgtgcagca cagggctgta caactggaac agcaactatc
     5101 gactattgca ttccctttca gtatgtagtt gacggatgtt atgcgcgcgc tcataaaatg
     5161 cgccagatca tgaatgaaga atataattat gaaccacaga aagttttcag ctatgaaggt
     5221 ccatcaggct ctcttgcagt agatgcgggc gattgctgtg tatactggtg gtaccatgtg
     5281 gcgccgcttg ttactgtaaa aacggctacc ggaactgcgc aatacgtaat agatccaagt
     5341 atgttcgatg agcctgtaag cattgctacg tggacggctg cacaagagaa tttgggttgc
     5401 acgccattcg ccgatttcgg atataccgag atcacagcag gaaaaattta tgcaccggga
     5461 ggttcaacag ataactcata tatcagcaca aactggacgc tgcagtttta tgctgatctg
     5521 gttacctgcc cgtaagttca gctattgcta taaacaaaaa gcgacctcat ttgaagtcgc
     5581 tttttttatg attaaccccc tgttaatatt aaaatagcat tgttgcacgc aagcctacct
     5641 gctgcgggaa ataacgtgag tcgttattag gattattggt gttcactacc atgcttttaa
     5701 taccattctg gaaaaagccg gtaaacacga gtgttttatc attgtcaagt ccaaattcgg
     5761 cacccgcgcc catgcttaaa ctggtattcg acccttcata gtcaaagctg ctgccaccgt
     5821 ttttgccatc gtttaccgtg attttctctg tcagcgtgaa tccgagtgtt agtccatatt
     5881 gagcaaataa aactgctttt cccaattcat ctgacctcaa acgtaataat ataggtaagt
     5941 ctaatgttgt aatactgtaa tcccaggctt ttccatttga tgccaagccc tccggattgt
     6001 ctttaaatgc acccggtgca ctggtaaagt tcaatcccga ttgcaaggca aaccgttcag
     6061 catcacctaa ggtgaggtct attgttccac caacctgaaa gcctactttc atgccatcag
     6121 gtacgtagtt atcgccttct actaaccaaa aagaaacagt tggagccaca ttggcgccgt
     6181 agcgaaattg ttgagcactt gccgcaacag ctaaagaaca gaatataatc gtgagtattt
     6241 ttttcattgt tattcgtata gaatgttgag aatgcctgtt taattaaaat agtcccgcaa
     6301 tgcgaatacc ggtctgattg agcaggatat cggaacctac attattgtct tcatacacat
     6361 tcatgattcc cttctgataa aagaatccga taatgataga agcaccttct gcaacttcca
     6421 cttcagctcc taagccaaac tgaagacctg cattcttatc aagataattt ataccgttag
     6481 cattgtcctt ttccaaataa tcaatatcgg agtcgagtgt aagcccgttc agagtataat
     6541 caccaacagc tctcaggcat ttaccataag tggctccacc ttctccatag attgtgaagc
     6601 tgccaaactg gggtgtcctg agcttcaatg taagagggat ttctaaatat cgagctctca
     6661 ctgcccattc tttatcgtcg cctgcaccat tgttgtcggt tgatgtcatg ccgctctgcg
     6721 agtagttcag attcaagcca agggaaacac caatgatgtc gcctttaaca aaggtttgat
     6781 ctaataataa gccgtattga aatccggaaa aggaaaccgc cggatcgtac atatctgcgg
     6841 gcagaacgag tttattgctg ccgggagcaa tgctgaagcc gtagcggaat tgctgagccc
     6901 ggcttgaaaa agctacgagc atcaggagga gaaaggtgaa tttacgcatg tctgaatttg
     6961 aaatttctac aagtggcaaa aatatatgct ggttcagcaa ttctcaggta ttttgatggc
     7021 attcatgtca taaaattgaa agaaatttgc aaacatggtt gttgataata ttgagttaca
     7081 gatcattttt agttaaaata tagatgattt gttatgtcac aaacgataaa aaacagacag
     7141 tattttagtt tttcagctgt tttctgttct tccaaaggaa agggtaattt gatgggtttg
     7201 cggctctttt catagccaaa ccgtaggtgt tttggttgag atctggcagg ggtacggcct
     7261 ttacaggagg aaaagcggca atttatttgg atttctgtgt acaacctcat atctttgcag
     7321 cccgtttatt cggaaacgac taaatgggac gaaaaaccgg ccgcaggttg attatgggag
     7381 tgaaccccga tatcgttcgc aaatggctta cctgcactct ctcctacaaa ttttgggggt
     7441 ttgtgcacat acctttgtga atatcctcac cccttcttat ctttgcagcc ccaaattttt
     7501 taaaacgaaa agggtaatta acgaatgcct actattcaac agttagtccg taaaggaagg
     7561 gacgtaatga aagcgcccag caaaagtcgt gcactggatg cctgtccgca aaaacgcggt
     7621 gtttgcacca gggtatatac taccacccct aagaagccta actcagcgct tcgcaaggtg
     7681 gcaaaagtgc gtttgaccaa tcagactgag atcatcgcct acattccggg tgaaggacac
     7741 aacctgcagg aacactctat cgtgcttatt cgcggtggta gggtgaaaga ccttccgggt
     7801 gtgcgctacc atattgtacg tggtgctctc gatactgccg gtgtaaacaa ccgcaagaaa
     7861 agccgctcta aatacggcac taaacgtcct aaagcaggtg ctgctccagc cggtaaaaag
     7921 taattaagta acagacagta gtccattata aacaatgaga aagcttaaac ctaagaaaag
     7981 gatactccct gccgacccgc agttcaacga tgcgacagta acacgcttcg tgaatatgat
     8041 gatgtggcaa ggtaaaaaaa gtactgccta tacctgtttc tatgatgcat tggcattggt
     8101 agaaaaacaa accggcgaaa gcggttatga ggtttggaaa aaagcattga gcaatgtaac
     8161 tccgggtgtt gaagtgaaaa gccgccgtat cggtggtgcc aacttccaga ttcctacaga
     8221 gatccgcccc gatcgtaaag tgtcactctc tatg
//
LOCUS       DAOQOU010000148         8251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_4779_length_8251_cov_5.070888, whole genome shotgun sequence.
ACCESSION   DAOQOU010000148 DAOQOU010000000
VERSION     DAOQOU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4779_length_8251_cov_5.070888"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1545)
                     /locus_tag="PLM90_11520"
     CDS             complement(358..1545)
                     /locus_tag="PLM90_11520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ90052.1"
                     /translation="MKRFATIMFAAMLGITGVQGQNSKITTAASAQYDREFGKAKQAI
                     DEACMHEKTKLEAKTWYMRGDIHNDIARDTTGRFNDIANPADTALASFKVALQQADVK
                     NYKLKIADGLFITYNIFFVKGANAYSAGNYEEAYTNFTKANEANLLQIDANPLASLDT
                     GVMFNMGLMAEKTNRTVEAIGVYQKLVDMKYGEPYLYSRLSSMYLDAGRQDDALKVLD
                     SGRKNFPTDKEIMTAELNYYLAQNKLGELVGKLENAIELDPKNAELYFVLGTTHGELV
                     KLDSLHGKQHFDAAVTAYNKALEIDKNRFDINLNAGALFYNTAIELNKRMNVLPLEME
                     AEFEKLKTERNSLYQNALPYFENAHAIEPKNTDCMIALKEIYVRLGQLDKAESIKQQL
                     DSN"
     gene            complement(1580..4138)
                     /gene="gyrA"
                     /locus_tag="PLM90_11525"
     CDS             complement(1580..4138)
                     /gene="gyrA"
                     /locus_tag="PLM90_11525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMZ90053.1"
                     /translation="MADGGRIIPINIEEEMKTAYIDYSMSVIVARAIPDVRDGLKPVH
                     RRVLYGMKDLGMAYNKPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSMRYTLV
                     DGQGNFGSVDGDSPAAMRYTEARFTRIAEEMMEDIEKDTVEFTNNFDDTLEEPTVLPS
                     RIPNLLINGSSGIAVGMATNILPHNLTEICDGIIALINNREITTTELMQYVKGPDFPT
                     GGVIYGVEGIIESFETGRGRIVVRGVANIETTANGREQIVITELPFQVNKALLISKIS
                     ELVHEKRIEGISDANDYSDREGIRVVIDLKKDAVATVVLNQLFKYTPLQSSFGVNNIC
                     LHAGRPKLMNLKTMIGHFVDFRHEIIVRRTRYDLKKAEERAHILEGLLIALDNLDAVI
                     ALIRAAKTPDEARDGLMTQFGLSEIQAKAILEMRLQRLTGLERDKIRDEYTEVMELIA
                     KLKEILDREDLRMQIIIDETLEIKEKYGDVRKTSIVAADGEMSIEDLIEEEDVAITIS
                     HLGYIKRTSLTEYRVQSRGGKGSKGGATRDEDFIEHIFIASTHDTMLFFTQQGKCFWL
                     PVYQIPEGTRVSKGRAIQNLISLPPEDKVMAYIDIHDLEDEAFINGHYLVFCTKKGTI
                     KKTTVEAFSRPRANGIVAINIEEGDELLEVRLTNGNCEIMLAVKSGRSIRFPEEKVRP
                     MGRTATGVIGIELANESDEVVGMITVEKGDKEKTVLVVSENGYGKRSYLVDPETGEDE
                     YRITNRGGKGVTTIKITPKTGNLIAIKDVTDRDDLMIICESGMTIRTGVEDLRVMGRA
                     TQGVRLINLRDGDSIASVTRVEKDDEEETSGAEGEEPNVDNPDTTSDTTSDNNE"
     gene            complement(4455..6242)
                     /locus_tag="PLM90_11530"
     CDS             complement(4455..6242)
                     /locus_tag="PLM90_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ90054.1"
                     /translation="MKILFQYLRPYRGLVILVLTLAAINTGFSLMDPILLGRLVNLAN
                     DFIGDKENYDWHRYIYSFSWNSPGVLLLLLSSVTVAMVSRIAKNFQDYFLNVVIQKFG
                     ASVFTDGLKHAMKLPYQEFEDQRSGETLSILTKVRTDVERFMNMAINVLLGILVGIVF
                     VFVYAAIFIHWYIPAVYLVGIVLLATISNVLSKKVKIIQKTIVGKTTALAGATTESLR
                     NIELVKSLGLTQQEVERLNRNTYKILGLELTKVKRIRSISFIQGTFVNTLRQVILFLL
                     MWLIFDDKMDAGQLVTMQVFSFFVFGPLQDIGNIILSYREAEASLLNFNNLMNKVPEP
                     EPASPKALGPIQHLQFDQVQFKHKTASTNAIDNISFEVNLGETIAFVGPSGSGKSTLM
                     KLLVGLYRPQNGKILYNGLNEFDINFNDLRNQIGFVTQDTQLFAGTIRENLMFVNPQA
                     TESDLQDVLQKASCNGLLSRAENGLDTMIGEGGLKLSGGEKQRISIARALLRRPRLLI
                     FDEATSALDSLTEEDISNTIREISAAGDQITILIAHRLSTIMHADRIYVLEQGEVEET
                     GTHDTLLENKGLYFAMWRQQIGGRKKVTV"
     gene            complement(6313..7869)
                     /locus_tag="PLM90_11535"
     CDS             complement(6313..7869)
                     /locus_tag="PLM90_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ90055.1"
                     /translation="MHSKIAHARFTVRSILKHDLPASISVFFVALPLCLGVSLASNAP
                     LYSGLLAGIIGGILVPFISKSALSVSGPAAGLTAICAAAIAATGDIQTFFLSVMLAGV
                     LQTLLGVFRLGGFTHFIPSAVIKGMLSAIGVMLISKQVPLLIGYDEPDFWTNAFFNVF
                     TLDHAFDNILTVINHTSAGAIIIAAICVGIIIVWNKLSKKPSSVPQAFIIVIIGSSVA
                     AIFKAYMPTLALHDGQFVRIPSHVISQITIPDPQLLFSDSVIWRNAVIICFVATLETL
                     LSITAIDKLDNKNRITPQNRELVAQGFANFISGMVGGLPITAVIVRSSANVQAGAQSK
                     WSAVLHGVWLLIAILFGVYLLDFISYCVLAVILIQTGYSLAKPHIFKSVYRQGNEQFM
                     PFIVTFFAILFTDLLIGVGIGISYAIYFLIKHTYRAGYSLQVIHHGHTTVYKIELALN
                     VSFMNKKRILDMLESIPSYSEVEINGVDSVYIDRDVLEVIQEYRSKAKSRHIQLTTFG
                     IAEVETLVAH"
     gene            complement(7953..>8251)
                     /locus_tag="PLM90_11540"
     CDS             complement(7953..>8251)
                     /locus_tag="PLM90_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90056.1"
                     /translation="TACNSTKQAGTTPAITYSGKVKTIIDENCANTCHNAARPAAGID
                     LTTYEKVKDQALNGKLIPAIQHAEGADPMPKKAAQLDDATIKVIVDWAASGALQ"
BASE COUNT         2222 a         1911 c         1629 g         2489 t
ORIGIN      
        1 tgaacacagg atttttcgct gcccgataaa aacaagcgga tttaccaagc ggcatacaaa
       61 tatacgagtt ttatgtgttt tttgtttcgc gtcagaaaca taatttattg attgtctaaa
      121 atattagacg ccagtttgcg cagccttctc cccactccgt tccgctcgta ccttatggct
      181 cgctccactc tgtgacctcc gaccctcctg ccgactgccc gttccacagc acaacgtcaa
      241 accaactata actaaacttt atgttaagta gaatataatg aagccgcctc tcaccatgtt
      301 cctaaaacaa aaaagggatc cgtaatggat ccccttttgt tcaatgattt tggtctatca
      361 attgctatca agttgttgct tgatcgattc agctttgtcc agttggccga ggcgaacata
      421 aatctctttc aatgcaatca tacaatcggt attctttggt tcgatagcat gagcattttc
      481 aaagtatggc aatgcgtttt gatagagact gttgcgttct gtttttagtt tttcaaattc
      541 agcttccatt tccaacggca acacattcat acgtttgttc aattcaatgg cggtattgta
      601 aaacaatgca ccggcgttga gattgatgtc gaaacgattc ttatcgatct caagggcttt
      661 gttataagcg gttacagctg catcaaagtg ctgcttaccg tgcaatgaat ctaatttcac
      721 caactcgcca tgggttgttc ccaatacgaa gtatagctct gcattcttag ggtccaattc
      781 tatagcattt tcaagctttc caaccaattc acctaacttg ttctgcgcca gataatagtt
      841 caactcggcg gtcatgatct ccttgtcggt tgggaaattc ttacgcccgc tatccaatac
      901 ttttaaggca tcatcctggc gaccggcatc aagatacatg ctcgacaagc ggctgtacag
      961 gtatggttcg ccatatttca tatccacaag tttctggtaa acccctatag cctcaacagt
     1021 tctgttagtc ttttcagcca tcagtcccat gttaaacatc actcctgtat ccaatgatgc
     1081 aagcggattg gcgtcgatct gaaggagatt ggcttcattt gctttggtga aattagtata
     1141 cgcttcttcg tagttgcctg ctgagtaagc atttgcgcct tttacaaaga agatattgta
     1201 ggtgatgaac aagccatctg caatcttcaa tttataattc tttacatccg cctgctgcaa
     1261 ggctacttta aacgatgcca gggctgtatc tgcaggattt gcgatatcgt tgaaccggcc
     1321 tgttgtatct cgtgcaatgt cgttgtggat atcgccacgc atataccagg tttttgcctc
     1381 caatttggtc ttttcatgca tacaagcctc atctatcgcc tgctttgcct taccaaattc
     1441 ccgatcatat tgcgcacttg ctgctgttgt gatcttactg ttttgtccct gcacgccggt
     1501 aataccaagc attgcagcaa acatgattgt cgcgaaacgc ttcatttttt tgggatttaa
     1561 aatattgata ttcaatttgt tattcattgt tatctgaagt agtatctgat gttgtatccg
     1621 gattatccac attcggttcc tcgccttccg ctccgcttgt ttcctcttca tcgtcttttt
     1681 ccacacgcgt taccgaagct atactgtcac cgtcgcggag gttgattaat cgcactccct
     1741 gtgtagctcg tcccatcaca cgcaaatctt ctacgccagt tctgatggtc attcctgatt
     1801 cgcaaatgat catcagatca tcgcggtcgg tcacatcttt aatggcgatg agattacctg
     1861 ttttaggggt tattttgata gtggtaacgc ctttaccgcc cctgttggtg atgcgatatt
     1921 catcttcacc tgtttcaggg tcaacgagat agctgcgctt gccatatccg ttttcactaa
     1981 ccacgagcac tgttttttct ttatctccct tctcaacagt aatcatgcca actacttcat
     2041 ctgactcatt ggcgagttca attccgatca ccccggtggc tgtacggccc atcggacgca
     2101 ccttttcttc cgggaagcga atcgatctgc cgcttttcac cgcaagcatg atctcgcagt
     2161 tgccattggt taagcgcact tctaacagtt catcgccttc ctcaatattg atggcaacga
     2221 taccattggc acgcggacgg ctgaaagcct caacagtagt tttcttgatg gtgccttttt
     2281 tagtgcagaa aaccaggtag tgaccattta taaatgcctc atcctcaagg tcatggatat
     2341 cgatatatgc catcactttg tcctcaggag gcaggctgat cagattctgt atcgccctac
     2401 ccttcgaaac gcgtgttcct tcaggtatct gatacaccgg taaccagaaa catttgcctt
     2461 gctgtgtaaa gaacaacatg gtatcgtggg tgctggctat gaaaatatgc tcaatgaagt
     2521 cctcatcgcg ggtagctccg ccttttgaac cctttcctcc cctgctttgc acgcggtatt
     2581 ctgtcagtga agtacgcttg atataaccta ggtggctgat cgtaatagca acatcctctt
     2641 cctcaatcag gtcttcgatg ctcatttcac catcagcagc tacaatcgat gtcttgcgaa
     2701 cgtcgccgta tttctctttg atctcgagtg tctcatcgat gatgatttgc atccgcaggt
     2761 cttccctatc cagtatctcc ttcaatttgg caatgagttc cattacctcg gtatattcgt
     2821 ctctgatctt atcgcgctca agccctgtaa gacgttgcaa acgcatctca aggatggctt
     2881 ttgcctgaat ttccgataac ccgaattgtg tcatcagccc atcacgagct tcatccgggg
     2941 tttttgccgc cctgatgagc gctatcactg cgtcaagatt atcgagggca atcagcaagc
     3001 cttcaaggat atgtgcgcgt tcttcggcct ttttcagatc gtaacgcgtt ctgcgaacga
     3061 ttatctcatg acgaaaatcg acgaaatggc cgatcatggt tttgaggttc atcagtttcg
     3121 gtctgccggc atgcagacag atattattca ccccaaaaga gctttgaagt ggcgtgtatt
     3181 tgaataattg gttgagcact accgtggcga cagcatcctt tttcaagtct attacgacac
     3241 ggattccttc gcgatcgctg taatcgttgg catcagaaat gccttcaatc ctcttttcgt
     3301 gcaccaattc agatatcttg ctgataagca atgctttgtt cacctggaat ggcagctcag
     3361 tgattacgat ctgctcgcga ccattagctg tagtttcaat attggcaaca ccgcgaacca
     3421 caattcgtcc acgaccggtt tcaaagcttt cgatgatgcc ttctacacca tagataacac
     3481 ctccggtcgg gaaatcggga cctttcacat attgcatgag ctcagtggtg gtgatttcgc
     3541 gattattgat caatgcaatg attccatcgc aaatttcagt gaggttatga ggaaggatat
     3601 tcgtggccat tcccaccgcg attcctgaag aaccgttgat cagcaggttt ggaatacggg
     3661 aaggtaaaac ggtaggctct tcaagggtat cgtcgaagtt attggtaaac tcaacggtat
     3721 ccttttcgat gtcttccatc atttcttcgg caatacgagt aaatcgcgct tccgtatatc
     3781 gcatcgctgc cggactatca ccgtcaaccg aaccgaagtt tccttgcccg tcgaccagtg
     3841 tatatcgcat actccaatcc tgagccatac gcaccatggc atcgtaaaca gagctgtcgc
     3901 cgtgtgggtg atacttacca agcacctccc ctacgatacg tgcggatttc ttgtaaggct
     3961 tgttataagc cataccaagg tctttcatgc cataaagcac gcgacggtgc acaggcttta
     4021 atccatcgcg tacatcaggg atggcacgcg ctacaattac cgacatcgaa taatcgatgt
     4081 aggcggtttt catttcttct tcgatattaa tcgggataat ccttcctccg tcagccatat
     4141 atgctacttt ttccgctttt ttttggtgct gtaaaggtag gcaatttcag cgcgaaagca
     4201 ccatattata gggctttcgg actgtcaagt tttccacatt tttcaaccgg aaacgggctt
     4261 gaaaatataa atgcaagttt gtgtgcgcag aaaaacaaaa acaggggtgc ccacgagcgt
     4321 ggcgcaaccc ctgatcccca tgaaaacaca cttgataata taaactcctt ttctaaccaa
     4381 aagtttcatt ggcattgtta aagatggtta aaacaatgcc gggaaaattg caggaatttg
     4441 aaggttgtag tttcctaaac agtcactttt ttccgcccgc cgatttgctg gcgccacatg
     4501 gcaaaatata atcctttgtt ttccaataag gtatcatgtg tgccggtttc ttcaacttct
     4561 ccctgctcaa gcacataaat cctgtcggca tgcattatgg tactcaggcg atgtgcaatt
     4621 aatatagtga tttgatcacc tgcagccgat atttctctga tagtatttga aatatcttct
     4681 tcagtaagtg agtctaacgc tgaagttgct tcatcgaaaa ttaatagtct cggtctgcgc
     4741 aacaatgctc tggcaatact gatacgttgt ttttctccgc ccgataattt caatcccccc
     4801 tctcctatca tggtatccaa tccattttct gcacgtgaca ataatccatt gcaacttgct
     4861 ttttgcaata catcctgcaa atccgattct gttgcctgtg gattcacaaa catgaggttt
     4921 tcgcgaatgg taccggcaaa taattgtgtg tcttgcgtaa caaatccgat ctgatttctc
     4981 aggtcgttga agttaatatc aaattcattc aaaccattgt ataatatttt tccgttttgc
     5041 ggtcgatata aaccgacaag caacttcatc aaagtgcttt ttcccgatcc tgaagggccg
     5101 acaaatgcaa ttgtttcacc aaggtttacc tcaaaactaa tattgtcgat tgcgttggta
     5161 gatgctgttt tgtgtttgaa ttgcacctgg tcgaactgca gatgttgtat tggacccaaa
     5221 gcctttggtg aagcaggttc cggctcgggt actttattca tcaggttgtt gaaattcagt
     5281 aatgatgctt ctgcttcgcg ataggataaa atgatattgc caatatcttg caatggcccg
     5341 aaaacaaaga atgaaaatac ctgcatagtt accaattgtc cggcatccat tttatcatca
     5401 aatatcaacc acattaataa aaataaaata acctgtcgca gggtatttac aaatgttccc
     5461 tggataaaac taatacttct gatgcgttta acttttgtta attccagtcc gagaatttta
     5521 tatgtatttc tatttaatcg ctctacctct tgctgcgtca atcctaagct tttcacaagc
     5581 tcaatatttc ttaaggattc agtggttgct ccggctaaag ccgttgtttt acccacaatt
     5641 gtcttttgta taatctttac ttttttactc aacacattag aaattgtagc cagcaaaacg
     5701 atgcctacca aatatacagc cgggatatac caatgaataa aaatggctgc atatacaaac
     5761 acaaatacaa tccccaccaa aatgccaagc agcacattaa tagccatatt catgaatcgc
     5821 tcaacatcag tgcgcacttt tgtcaaaatg gaaagtgttt cgccactgcg ctgatcttca
     5881 aattcctgat atggtaattt catcgcatgc tttaaaccat cggtaaaaac acttgcacca
     5941 aatttttgaa ttacaacgtt gagaaaataa tcctggaagt ttttagcaat tctgctcacc
     6001 atggcaacgg taactgagct gagcaggagc agcaaaacac ccggactatt ccaggaaaag
     6061 ctgtagatat agcgatgcca gtcgtaattt tctttatccc cgataaagtc gttggccagg
     6121 ttcaccaatc gacccaaaag aataggatcc atgagtgaaa accccgtatt tatagctgca
     6181 agtgtgagca caaggatgac caagcctcgg taaggtctaa ggtattgaaa caaaattttc
     6241 ataatagtat gccgggtttc aactcaaaac taagctataa ggttcgttga atagtaatta
     6301 ttttatcaag aatcagtgag ctaccagtgt ttctacttcg gcaataccaa aagtggtgag
     6361 ctgtatatgt ctggattttg ccttcgacct gtattcctga atcacttcca atacatctcg
     6421 gtcgatgtat acactgtcca ccccattgat ttctacttcg ctgtatgaag gaattgattc
     6481 caacatatct aatattcttt tcttattcat gaagctgaca ttcaatgcaa gttctatttt
     6541 atatacagta gtatgtccgt gatgaattac ttgcaatgaa tagccggctc tgtatgtatg
     6601 cttgattaaa aaataaatgg catagctgat acctataccc acaccaataa gcaaatcagt
     6661 gaataaaata gcaaaaaatg tcacaataaa tggcatgaat tgctcattgc cctgtcggta
     6721 tactgattta aaaatatgcg gttttgcaag cgaatatccg gtttgaatta atataactgc
     6781 aagtacacaa taagaaatga aatccaacaa ataaacgcca aataatatgg caattaacaa
     6841 ccacacgcca tgcaataccg ccgaccattt cgactgtgcg cctgcctgca cattcgcaga
     6901 gctgcggaca attactgcag tgatgggcaa accaccgacc atgccactta taaagtttgc
     6961 aaatccttgt gcaacgagct ctctattctg cggagtgatg cgatttttat tgtccagttt
     7021 atcaattgca gtaatactga gtagcgtttc caaagtggca acaaaacaaa taatgacagc
     7081 attccgccag attacactat cgctaaaaag cagttgagga tcagggatgg ttatctgact
     7141 aattacatga gaaggaatgc gcacaaattg cccgtcatgc agggcaagtg taggcatata
     7201 tgccttaaat attgctgcta cagaagatcc aataattacg atgataaatg cctgtggaac
     7261 agaggatggc tttttcgata atttattcca tacaataata attcctacac atattgctgc
     7321 aataattatt gcccctgcag aagtatgatt gataacagtt aatatattat caaacgcatg
     7381 gtcaagtgtg aaaacattga aaaacgcatt cgtccaaaag tccggttcgt cataaccaat
     7441 cagcaatgga acttgtttgg agataagcat tacacctatt gccgataaca ttcctttaat
     7501 aacagcactt ggaataaaat gtgtgaatcc tcccaatcgg aaaacgccta ataaggtttg
     7561 taacacaccg gcaagcatta ctgatagaaa gaatgtttgg atatcgccgg tagcggctat
     7621 tgcggcagca catattgcag ttaatcccgc agcaggtcca cttacactca aagcagattt
     7681 gctaataaaa ggcaccagaa taccacctat aatacctgca agtaatccgg aataaagcgg
     7741 tgcattactt gctaatgaaa cgccaagaca aagtggcagc gccacaaaaa atacacttat
     7801 gcttgccgga agatcatgtt tcaggattga tcttacggta aagcgtgcgt gcgcaatctt
     7861 actatgcatg ttatcacaaa ggtgcagcac tccttaccca attgggtagg aaattatgca
     7921 accttttctg tgtataaccg cctgtaagaa gcttattgaa gagctccgga agcagcccaa
     7981 tctactatca ctttgattgt tgcatcgtcg agttgtgcgg cttttttggg catcggatct
     8041 gccccttctg catgttgaat ggcggggatc agtttgccat taagtgcttg atccttcacc
     8101 ttttcatatg tcgtgaggtc gattcctgcc gcaggtcggg ctgcgttatg acaggtgttc
     8161 gcacaatttt catctatgat ggtttttacc tttccgctgt aggtaatagc cggagttgtg
     8221 cctgcttgtt ttgtggaatt acaagcagta a
//
LOCUS       DAOQOU010000149         8006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5008_length_8006_cov_5.525217, whole genome shotgun sequence.
ACCESSION   DAOQOU010000149 DAOQOU010000000
VERSION     DAOQOU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_8006_cov_5.525217"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..543
                     /locus_tag="PLM90_11545"
     CDS             223..543
                     /locus_tag="PLM90_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90057.1"
                     /translation="MFRLFAFSVTFIGVLSIGSAQSLSDLKMTLPSLEERYKMDYDIQ
                     GLFNPSADTLADLNLEQYEELRQVDNDVEVYDPATGFKITIYSVNKAIENYTYRFKKD
                     EEDQ"
     gene            568..2043
                     /locus_tag="PLM90_11550"
     CDS             568..2043
                     /locus_tag="PLM90_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90058.1"
                     /translation="MVKNIFITLLVCIALADLQAQTYLHPTSGIDGTYVGACMVNTCS
                     GNYYDDGGAASNYSNNISSYYRVFCPNTAGNCITATFTSFDVQNDCTKDYLTIGNGAT
                     QNSTTIAIPGVTTVAGGANRICGTPPVPFSATATNASGCLVMRFTSNASSNAAGWAAT
                     ISCSACAIGPVATDPNDCINAIAICDDLPVTGVESGPGLVSESCSGCTAGGENYSTWY
                     YFCFETSGTFGFTIDPVTNTDDYDFALYGPDVSCGALGTPVRCSYAASTGNTGLKATV
                     SDVSETVTGNGWVDTLHVTAGECYYLMVNHWSPPVTGYTIDWDLTDGATFVDCDLLPV
                     TLTSFYAEAYDNNTVSLTWETETEFNNDYYIIERSTDGQTFEPFMQLDAKGNSSDKTT
                     YFAVDAHPNLGINYYKLSQVDLNGRTTYLKTASVDVQYNNSFGTISIYDLSGRIIHSE
                     VVYRSQIAVMLDAIQLDPGVYVYRYLQENGVSENGKFSVVR"
     gene            2234..3049
                     /locus_tag="PLM90_11555"
     CDS             2234..3049
                     /locus_tag="PLM90_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90059.1"
                     /translation="MQRSILCITAMTIFTTWACSCSDKDLNERKAISDQLTTVAIENT
                     LLLIRQTIGFERQIESEALDRFKSQNYFKMLAVDSAISSIIYGIKTYNQQFDKTTLRR
                     HKDRVMRIANLDEYEEKYVSENMLSTYDTLGDFSFPNEAILHMLQFEFYVQKIMSRRS
                     SYCGNIDNGHLTKLDEHTYAVSIQWNDHRFYFMDSMDVNYGWINYHFIRRDQMKVYHG
                     GTYVLITFESPTPFKEEFIYAGIAYKRKNGVTGYLDDQYYALFTNVVNEDKNP"
     gene            3090..3893
                     /locus_tag="PLM90_11560"
     CDS             3090..3893
                     /locus_tag="PLM90_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90060.1"
                     /translation="MKRYIHAIYTSCIIYCMGCSTPEHNNKDELIRSSYAVVEAYNNM
                     LNRNAIYKLQNDSREESQLILNTLETMYPQAGYSVPSRFISSDYFSQLDSLVDQEPYT
                     SLPMKNLREAIVKWRYDLHQMKRPLSLDEARLMRAMLDNLLFDYANRIIQSDCFWGLR
                     EIPYVANNGNIIITDPHSFNDQVMRNAVIKNVQVLINDENISPVAFRHSTTLNPLIFE
                     IDKHAVELQEENVFRINYTIENVMFKSGSEQQIWQTLTSSEYYNQIQWK"
     gene            3890..4687
                     /locus_tag="PLM90_11565"
     CDS             3890..4687
                     /locus_tag="PLM90_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90061.1"
                     /translation="MRTATLLLMASLFVCALSCKQVERPEHMTGTQILAMQAIDATEN
                     QISQWIGITRYHSQRSSHAGLQDIYDNMLSISKASDNAIDGINSGKLSFRQDSLSLIK
                     RNIIRSQSYSNESRRYWRDSMVYSFDTLPVFSCKEEAILSIKLLELMMIRDLGRSYTV
                     CTEYEDAPLFWIDDHTVVAILGSKAINMFTKLDTIHIISGWIANNPLTPEQYHVQISG
                     NYCLITIAVPDDYKQPFIEAGFEYVFNAEDRAYSRRCYASFAGYKEK"
     gene            4785..5687
                     /locus_tag="PLM90_11570"
     CDS             4785..5687
                     /locus_tag="PLM90_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ90062.1"
                     /translation="MKRFYSTLITCICITVIQAQQVHVSSGKVIRHANFPSQFVPPRN
                     VDVWLPDNYDATKSYAVLYMHDGQMLFDATTTWNKQEWQVDEHMGKLLQSGEIRDCIV
                     VGIWNVPQLRHSEYFPAKAVPYIEKTLIDSLKKVEWESALPTSDAYLSFIVRELKPFI
                     DNNYATKTDKANTFIGGSSMGGLISLYAICEYPAVFGGAICMSTHWQGSLKVLDDNIS
                     TGMLKYYRKHLPNPATHKIYFDYGSETLDKAYKPFQLIMNKAMNKRGYTFEHWMTREV
                     PGADHSELAWSARLEIPLVFLLAE"
     gene            5868..7355
                     /locus_tag="PLM90_11575"
     CDS             5868..7355
                     /locus_tag="PLM90_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90063.1"
                     /translation="MYFSFQLLHQLTADERKLVENASLTSRERELMDFMLTQHSKHFP
                     AVKALHALKISRTNLYKLNSNLLTNLLEIIAPGNILVKLEYLMNRSSVYALRSQLLKQ
                     HHEKIKHSATQADHTFYREFYKWICSDNSSDTIKELQSEITAMVIATSKPEAEKEVKL
                     FIDLNAFRVEMFKKWRNGTIENNSKFKELKERGYSIINSARQYGDKIIIRSTYDSCIF
                     MFISNKEFEDAKSLLEEFNILIKSEPETYSDISNWYVGSMKGVIEYTQLNMDLAYSYF
                     SKHLYLTLDHPIHSQNNIYARYFITTLLLNKSEIAREIYNKWNMQFSFDPRISLYTNH
                     LQIKTMFEIFNEKYDSAIATLNILELNGWYNYGFSLQYFLREYRLSIAYLTDDFGTAH
                     ALSEKNLTYIRYSKRNKIQPEVAFHSKFVKVLIKCIRKKRQLHKSEFGMLVKLIEYHP
                     LHALTLQRCFNKYQVLFNNEPLVVWEPEKGISDLVLEYIKMPEPA"
     gene            complement(7788..>8006)
                     /locus_tag="PLM90_11580"
     CDS             complement(7788..>8006)
                     /locus_tag="PLM90_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90064.1"
                     /translation="VHDILTVMHDWQSTEGVQIVVLNELGQAVLTKDFAAGDKITVDV
                     TALPGAVYFIRLIDLNSEIIYSGTFVKE"
BASE COUNT         2598 a         1608 c         1495 g         2305 t
ORIGIN      
        1 aattctttgc tactgccgga ggtaaaaaca caggaggatt gaggaaggca ttggattctg
       61 tgacaacgtt actgtaagtt ttatcaggca aattactgca agttttcccg atttcaagcg
      121 ctttttaatc gtggaaatat tgtggatttg gcaaatttgt gacatcgaaa ccttgcgaaa
      181 gcgccaattg caggttaatt ttgattaaaa tttgatattt ccatgttcag gctattcgca
      241 ttttcagtca cctttatagg tgtgctatca attggtagtg cccaaagtct cagcgatctg
      301 aaaatgacat tgccatcatt ggaagaacga tataaaatgg actatgatat tcaaggtctg
      361 tttaatcctt ctgcagatac attggctgat ttgaatctgg agcaatatga agaacttcga
      421 caagtagata atgatgttga agtgtatgac ccggccactg gttttaaaat cacaatctat
      481 agtgtaaata aagcaattga aaattatact tatcgtttca aaaaagacga agaagatcaa
      541 taatttcctc tcctcacacc ctcacacatg gtaaaaaata tttttataac attattggta
      601 tgtattgcac ttgccgattt acaagcgcaa acatatttac atccaacctc gggtatcgat
      661 ggaacttatg tcggtgcttg tatggtaaat acctgctccg gaaattatta cgacgacgga
      721 ggggctgcaa gtaactatag taataatatc agttcttact acagagtatt ttgccctaat
      781 actgccggca attgcatcac agcaacattt accagctttg atgttcaaaa tgattgcaca
      841 aaagattatc tcaccatagg aaacggtgct acacaaaata gtacaacaat tgccattcct
      901 ggagttacaa cagttgcagg tggagcaaat agaatttgtg gaacgccacc tgtgcctttt
      961 tccgctactg ccaccaatgc cagcggttgt ctggtaatgc gctttacctc caatgcatca
     1021 tcaaatgctg ccggatgggc ggcaactatt agttgttctg cctgcgcaat cggcccggta
     1081 gcaacagatc ctaatgactg tataaatgcc attgcaattt gcgatgatct tccggtaaca
     1141 ggcgttgaaa gtggtcccgg actagtttct gaatcatgca gtggttgcac tgccggaggt
     1201 gaaaattatt caacctggta ctatttctgt tttgaaactt caggcacttt tggatttact
     1261 atcgaccctg taaccaatac cgatgattac gattttgctt tgtacggacc tgatgtatca
     1321 tgcggtgctt taggtacacc ggttcgatgt tcctatgccg caagtacggg aaacaccgga
     1381 cttaaagcta ccgtttcaga tgtttcagaa acagttacag gcaacggctg ggtagacaca
     1441 cttcacgtta ctgccggcga atgttactat ttgatggtaa atcactggtc gccaccggta
     1501 acgggttata ccatagattg ggatcttact gatggcgcta cttttgttga ttgcgattta
     1561 ttgccggtta ccctcacctc attctatgca gaagcttacg ataataatac tgtttcattg
     1621 acctgggaaa cagagaccga attcaacaat gattattata taattgagcg ttcaactgac
     1681 ggtcaaacat ttgaaccatt tatgcaatta gatgcaaaag gcaattcatc agataaaacc
     1741 acttatttcg ctgttgacgc acatcccaat ttaggcatca attactacaa gctttctcaa
     1801 gtggacttga acgggagaac tacctatctc aagactgcat ctgttgatgt tcaatacaac
     1861 aactccttcg gaacgataag catttatgat ttatcaggaa ggattattca ttctgaagta
     1921 gtttaccgaa gccagatagc agtaatgctc gatgccattc aattggatcc cggcgtatat
     1981 gtgtatcgct atttgcagga aaatggtgtg tctgaaaacg ggaaattctc tgttgtaaga
     2041 taaatctccc tctattgaat acttattgat tatacgatta ctttaaagta attcgtttac
     2101 atggtaatta cccaaaacgc tgcggtcaat tgccgcacgt gctgtgttcg tttctgcgat
     2161 ttaaggctaa atcagacttc ctaaaaaacg ccattttagg aaggaatttt cacatttgtg
     2221 caaactgacc ttcatgcaga gatctatttt atgtatcacc gcaatgacca tctttactac
     2281 atgggcatgc agctgcagtg ataaggattt aaatgaaaga aaagcgatca gcgatcaact
     2341 tacaacagtt gcgattgaaa atacattatt attgattcgt caaacaatcg gttttgagcg
     2401 acagatagaa agcgaagctc tcgaccgatt caaatcgcag aactatttca aaatgctcgc
     2461 agtggatagt gccatctcat cgataattta tgggatcaaa acttataacc aacaattcga
     2521 taaaactaca ttacggcgtc ataaagatcg tgtaatgcgc attgccaatc ttgacgagta
     2581 tgaggaaaaa tatgtgtcgg aaaatatgct atcaacttat gacacacttg gcgatttcag
     2641 ctttcctaat gaagccattc tccatatgct tcagtttgaa ttctatgttc aaaaaatcat
     2701 gtcaaggcgc agtagttatt gcgggaatat cgacaatgga catttaacaa agttagatga
     2761 gcatacctat gcagtttcta tacaatggaa tgaccatcga ttttatttca tggattcgat
     2821 ggatgtgaat tacgggtgga tcaattacca tttcatccga cgcgatcaga tgaaggttta
     2881 tcatggcggc acctacgtat tgatcacatt cgaaagccct acacctttca aagaagaatt
     2941 tatatatgcc ggaattgcat acaaacgcaa aaatggggta acaggttatt tagatgacca
     3001 atactatgcc ttatttacaa atgttgtcaa tgaagacaaa aacccataac ataaaaataa
     3061 actcattgta taataactac aaattcaaaa tgaaaagata catacatgct atttatactt
     3121 catgcataat ttattgcatg ggttgttcta cgcctgaaca taacaacaag gatgaattaa
     3181 tacgttcatc atacgcagtc gttgaagcat acaacaatat gttaaatcga aatgcgatat
     3241 acaagcttca aaatgattca cgggaagaaa gtcaattgat tctaaacacc ctggagacta
     3301 tgtatccaca ggctggttac tccgtacctt ccaggttcat cagtagcgat tatttcagtc
     3361 aactagattc acttgtagat caagaaccct atacgtcatt accaatgaag aaccttcgcg
     3421 aagctatcgt aaaatggcga tacgaccttc accaaatgaa gcgaccactt agtcttgacg
     3481 aagcaagatt aatgcgcgcg atgcttgaca atttactatt tgattatgca aatagaatca
     3541 ttcaatcgga ctgtttttgg ggattgagag aaatacctta cgtggcaaac aatggtaata
     3601 ttataattac agatccgcat tcctttaatg atcaggtaat gcgaaatgct gttatcaaaa
     3661 atgtacaggt tctaatcaac gatgaaaata tttcacctgt tgcattccga catagcacaa
     3721 cgctaaatcc cctgatcttt gaaattgaca agcatgcagt tgaattgcag gaagaaaatg
     3781 ttttcaggat caactataca atagaaaacg taatgttcaa atccggatca gaacagcaga
     3841 tttggcaaac gcttacatca agtgaatatt acaatcaaat acaatggaaa tgagaaccgc
     3901 tacattatta ttgatggcat cactgtttgt ttgtgccctg agttgcaaac aggtagaacg
     3961 acctgaacat atgaccggca ctcaaatttt ggcaatgcag gcaatagatg ccaccgagaa
     4021 ccagatatca caatggatag gcataacgcg atatcattca cagcgaagca gtcatgcggg
     4081 attgcaagat atctacgaca atatgttatc aatttccaag gcatctgata atgcaataga
     4141 tggaattaat tcaggcaagc tttccttccg gcaagattcc ctatcactta taaaacgaaa
     4201 tatcatcaga tcacaatctt attctaacga atccagacgt tattggagag attcgatggt
     4261 atacagcttc gacaccctgc ctgtattttc ttgtaaggag gaagcgatcc tgagcataaa
     4321 attattggaa ttgatgatga tcagagacct tggaagaagt tacaccgttt gcacagaata
     4381 tgaagacgcc cccttattct ggatcgatga tcacactgta gtagcaatac ttggtagcaa
     4441 ggctataaat atgttcacaa agcttgatac cattcatatc atatcaggat ggattgccaa
     4501 caatccctta acacccgaac aataccatgt ccaaatctcc ggcaattatt gcctgattac
     4561 aatcgctgtt cccgacgact ataaacaacc cttcatagaa gccggttttg aatacgtatt
     4621 caacgcggaa gatcgagctt atagtcgcag gtgctacgca tcctttgcag gctacaaaga
     4681 aaaataggta tactttgcac taagcttttc aattcctatt tttacaacag gagcatcgtt
     4741 tgcccgtaaa atgaccggct agatttatgc tcctaccact cattatgaaa cgcttttatt
     4801 caacgctcat cacatgtata tgcattactg tgattcaagc acaacaggtt catgtttcat
     4861 ctggaaaagt aatcagacat gccaatttcc catcacaatt tgtgccacca cgcaatgtag
     4921 atgtttggct gccggataat tacgatgcaa caaagtcgta cgcggtatta tacatgcacg
     4981 acgggcaaat gcttttcgat gccaccacaa catggaataa gcaagaatgg caggttgacg
     5041 agcacatggg gaaactgctt caatcgggtg aaatccgcga ttgcattgtt gttggcattt
     5101 ggaatgttcc gcaattacga cattcggagt attttcctgc aaaggcagtt ccctatatcg
     5161 aaaaaacact aatcgacagt ctcaaaaagg tggaatggga atcagccttg ccaacttcag
     5221 atgcatattt atcattcatc gtacgcgagc tcaaaccatt tatcgataat aattacgcta
     5281 caaaaacaga caaagcaaat acctttatcg gcgggtcaag catgggagga ctgatttcac
     5341 tctacgccat ctgcgaatat ccggctgttt tcggaggtgc gatttgtatg tcaacgcatt
     5401 ggcaagggag cttaaaggtg ctcgatgaca atatttctac cggcatgttg aaatattacc
     5461 gcaaacacct accaaatccc gctacacata aaatttactt tgactatggc tcagagacat
     5521 tggataaagc ctataagccc ttccaactca ttatgaacaa ggctatgaac aaacgaggtt
     5581 atactttcga acattggatg acaagagagg tgccgggtgc cgaccatagt gaattagcct
     5641 ggagtgcgcg tttagaaata ccattggtgt ttctgcttgc agaatgacgt ctaatcttgt
     5701 tttgttccct caggtcccat ttttcatgat tttagcgcaa tatcttacgc atgacgtaag
     5761 gattacacta aaatattttg aaaaatcacg ttaatgttaa ttttggagca gcaactatat
     5821 tagagggagc aatatagctt gccatacaaa aacggggatt taattatatg tacttcagtt
     5881 tccaattgct tcatcaactc actgctgatg aacgaaaact tgtagaaaac gcatcgctta
     5941 cctcacgcga acgcgagtta atggacttca tgttaaccca acattcaaag catttcccgg
     6001 ctgtcaaggc tttacatgcg ctaaaaatat cccgcaccaa tttatataaa ttaaattcca
     6061 acttacttac aaacctactg gaaattatag ctccgggcaa cattctggta aagttagaat
     6121 acctgatgaa tcgcagtagt gtatatgctt tgagatcgca gttgctgaaa cagcatcatg
     6181 aaaaaataaa gcattctgca acacaagcag atcacacctt ttacagagag ttttataaat
     6241 ggatatgcag tgataattca agtgatacga tcaaagaatt acaatcggaa atcactgcca
     6301 tggtcatagc cacatccaaa cccgaagcgg aaaaagaggt aaagctattc attgatttaa
     6361 atgcctttcg tgtagaaatg ttcaaaaaat ggcgtaatgg cactattgaa aacaattcaa
     6421 aatttaaaga gttaaaagaa cgaggttatt ccataatcaa ttcagcaagg caatatggcg
     6481 ataaaattat aataagatcg acttacgaca gttgcatttt tatgtttatt tcaaacaagg
     6541 aatttgaaga tgccaaatca ttacttgaag agttcaacat cctaataaaa tcagagcctg
     6601 aaacttattc agatattagc aattggtatg tgggatcgat gaaaggggtg attgaataca
     6661 ctcaattaaa tatggatttg gcgtatagct atttttcgaa acacctttac cttacacttg
     6721 atcaccctat tcattcccaa aacaatatct atgcgcgcta ttttattacg acactattat
     6781 taaacaaatc ggagatagcc cgcgaaatct ataacaaatg gaacatgcaa ttcagttttg
     6841 atccaaggat atcgctatat acgaatcatt tgcaaatcaa aacaatgttt gaaatcttta
     6901 atgaaaagta tgattcagct atagcaacat taaatatttt agaattaaat ggatggtata
     6961 attacggatt tagtttacag tactttttga gggaatatcg attgtctatc gcctatctca
     7021 ctgacgactt tggaacagct catgcacttt ctgaaaagaa tctaacctat atcaggtact
     7081 ctaaaagaaa taaaatacaa cccgaagtcg cattccactc taaattcgta aaagtgctga
     7141 taaagtgcat tcgcaaaaaa aggcaattac ataaatctga atttggaatg cttgttaaac
     7201 ttatagaata ccaccccctt cacgccttga ctctccaacg atgttttaat aaatatcaag
     7261 tgctttttaa taacgaacca ttagttgttt gggagcccga aaaaggcatt tcagatcttg
     7321 tattggaata cataaaaatg cctgagccgg cataacacca tctcaggcaa attagtcaat
     7381 ctaacatgac agaattacgc tcctggctcg actgacttta tgaaaaaaca cgacttataa
     7441 tgcatcccgt ttactagaac taatagaagt atatttcact aactcttatt ccgctgaatc
     7501 gccacgtgac atattgataa acaatgccat gttatgcatg tcacgccaga agtatatcac
     7561 acgtctcttc tttaacagct cagcaggcgg caactccaaa ttagcatcag gaggtcgcat
     7621 tgccgacaaa ttcgcaattt gacgaattcg actttgcaca cgtttttctt tcatttttgg
     7681 cttcattgca ttcaattata acccaaagaa atgcaatagg cgaagaaata aattgcaata
     7741 aaatcacaaa atcttacgta aagggaatca atactacttc agttggttta ctctttaaca
     7801 aaagttcctg agtatatgat ctcactgttt aggtcaataa ggcgtatgaa ataaacggca
     7861 ccaggtagcg ctgtcacatc aactgttatt ttgtcgcccg cagcaaaatc ctttgttaaa
     7921 actgcctgac ccaattcatt tagcacaaca atttgaacac cctctgtgga ctgccaatca
     7981 tgcattactg ttagaatatc atgcac
//
LOCUS       DAOQOU010000150         7999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5014_length_7999_cov_5.684038, whole genome shotgun sequence.
ACCESSION   DAOQOU010000150 DAOQOU010000000
VERSION     DAOQOU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5014_length_7999_cov_5.684038"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLM90_11585"
     CDS             complement(<1..679)
                     /locus_tag="PLM90_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ90065.1"
                     /translation="MDVIEFVDDSGTVLVKRIPDSGHLEIIWGSQLTVRESQEAVFFR
                     DGKALDVFGAGRHILKTQNVPVVGKWVTSFGYGENSPFRAEVVFVGKQLFANLKWGTR
                     EPILFRDTELNAVRLRSFGSYSIQVSDTMLFVNKVVGTKGLFASNSIEDYLKGIIVSK
                     LNTVLSNELKSVFDISKSIDQLNLILRTELQTDFNGLGLQIHDFYIQSISVPDEVQKI
                     IDSKSGMG"
     gene            complement(682..1164)
                     /locus_tag="PLM90_11590"
     CDS             complement(682..1164)
                     /locus_tag="PLM90_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90066.1"
                     /translation="MTTRRTNLFEKIGNMIRGYKGYSLRDEKRNTDKKLRDELSGIIQ
                     QSEKTVITHQQQLLKTGEMQLCQEWEIARKALNTIFSKIKNATYGESSFFSDQQLKET
                     ELDEIYKIDLEMSERVHLILKTVEEEINETMSAGFVNQQVREIDAILIKRTNFINQFK
                     "
     gene            complement(1778..2155)
                     /locus_tag="PLM90_11595"
     CDS             complement(1778..2155)
                     /locus_tag="PLM90_11595"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HMZ90067.1"
                     /translation="MWQRIQSIFLALAAIAAIAFLFVDIADGLTGKDNVAASAVAVGI
                     ALLQGFIIAMYKDRRVQMRFCATTMLLAAVMAALAYMKTDGENLRLAFIFGIPAVIMI
                     TSFLARYNIKKDEDLVKSMDRMR"
     gene            complement(2263..3471)
                     /locus_tag="PLM90_11600"
     CDS             complement(2263..3471)
                     /locus_tag="PLM90_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMZ90068.1"
                     /translation="MKIATTPSRLAAYSTMAATVLAIQNEADGQVIYTNINPDKVLQS
                     TGPYDTLHLDVTGDAITDFIFNAFYFYTSYTASAVNYDHLGVDGINGARIAYTMQSTN
                     IYTWSSALFANLSAKFAQILPANTPINNTLNFNNNVELFYQQCFPAMYSSMMDYCWTA
                     GMPSAVVKYIGFRLKNGPLKYYGWMRLSIHKSTPFVPVPTSIKIYDYAINWTPNTPIL
                     AGEGLICIATDAVGTGTTASHWAKVYWEPRVDIDHYEVSYRIPGGAWETKTVPPDKTS
                     TKLNGLECSTDYEWQVRCACLDGSLSDYSEVQTFTTASCRLEGEDMTENDEAVSMYAN
                     ANRIYIYFDEVVTEPSRLFIYNMTGQLVSETIVTQQQNILEVSVPSGMYTAKLVTPSG
                     NIGKLISISN"
     gene            3782..4537
                     /gene="truA"
                     /locus_tag="PLM90_11605"
     CDS             3782..4537
                     /gene="truA"
                     /locus_tag="PLM90_11605"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMZ90069.1"
                     /translation="MRYFAEIAYRGTHYVGWQIQPNGVSVQQKVNEALRKILRDEEIA
                     CVGCGRTDAGVHATQFFLHYDTFAEIPETFMLRMNTVLPDDIVVKSHFPVAENAHARF
                     DATLRAYEYFISFNKDAFGPDRVLWRHFRDLDFDLLQACCEVMKKYNDFPSFCKSKQG
                     SKTTIINLQEIYWRQEGDTYILFISADRFIRGMVRLVVGAMMQVARGKMTIEEFESGI
                     QKGERLKLALSAPAHGLYLMRVEYPYQFGTLPE"
     gene            4635..5138
                     /locus_tag="PLM90_11610"
     CDS             4635..5138
                     /locus_tag="PLM90_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HMZ90070.1"
                     /translation="MAAVKQTLEKKLDKEIGIYFQTTVTKEFKFDDKKRITYEDFLSD
                     RMLIIAAIRVGIPYSLFELIQIYTPFSENDWADLLDVSTKSLQRYRQASQHYFKSFHS
                     EKIIEMAEVTKLGLEVFEDMDKLKLWLNTPNFALGKIKPIELLKDSYGKDLVTSELVR
                     INHGILV"
     gene            5142..5600
                     /locus_tag="PLM90_11615"
     CDS             5142..5600
                     /locus_tag="PLM90_11615"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HMZ90071.1"
                     /translation="MEVFRLAREKYAHALSGKGSAIKGARWNSVGVELIYTASNRSLA
                     MAEVAVHLTLATLPGDFRMFTIHIPDTIAIKEVKMKYLPTNWQEFPHPATTQRFGDEF
                     VAANKYCVLKIPSVVTQGDFNYLINPYHADFKKIKIIEIVNFPFDKRIVK"
     gene            complement(5614..7911)
                     /locus_tag="PLM90_11620"
     CDS             complement(5614..7911)
                     /locus_tag="PLM90_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ90072.1"
                     /translation="MSSFLDELNEAQRAAVTNVSGPTMIIAGPGSGKTRVLTYRVAYL
                     METGIDPFSILALTFTNKAAREMKNRIEKVMGGDARSLWMGTFHSVFSRILRSEADKL
                     GYPQNFTIYDTDDAKNLLKNIVKEMGLNDKLYKPGYCLYRISLCKNNLIGPAEYANNT
                     DFIAEDEANGRPKMSEVYQQYAKRCFRAGAMDFDDLLLKTHELFERFPDSLYKYQHRF
                     KYVMIDEFQDTNHLQYAIVRRLADVYQNIAVVGDDAQSIYAFRGANIANILNFEKDFP
                     EVQTFRLEQNYRSTKVIVEAANQIISKNRHQLAKTIWTDNTEGDTIKVIRAMSDNEEG
                     RLVADQIFEQRMRDHFKNGEFAILYRTNAQSRSFEEALRRLNIPYRIYGGLSFYQRKE
                     IKDLVAYLKLTVNPNDEESFRRVINYPARGIGKTSFEKLTVFAADNEKSLFTMAENID
                     LLPFNAGTRKLFTDFITMIRSFQTMLETKDAYEVASHIAKSTGILKELYNDQSVEGLS
                     RYENVQELLNSIKEFASPDEANSPYFTETLPTENTGPRSLAEYLQQVVLLTDADQEGN
                     EDETVKLMTIHAAKGLEFKSVFVVGLEENLFPSALSVNSRDELEEERRLFYVAVTRAE
                     KKLVLTHAASRYKYGNLEYCEPSRFLEEIPQNLLTYVGYNKTSRQDERNIFDKAYEHA
                     TPIAKAKPVAPVQNYVHAPSAGFKVAFPEELKAGMEVEHQKFGFGTISALEGQNDGLM
                     ATINFHKAGQKKLLLKFAKLAIVEK"
BASE COUNT         2292 a         1701 c         1521 g         2485 t
ORIGIN      
        1 cacccattcc acttttggag tctatgattt tttgaacctc atcagggact gaaattgact
       61 gaatgtagaa gtcgtgaatt tgtaatccaa gaccgttaaa atctgtttgc aattcagttc
      121 ttaaaatcag atttaattgg tcaatgcttt ttgaaatatc aaaaacagat ttcaattcat
      181 tgcttaagac tgtgttcagc tttgaaacaa taattccctt caaataatct tcaatggaat
      241 tagaagcaaa tagtcctttt gttcctacaa ccttatttac aaataacatt gtgtcgctta
      301 cttgaattga ataactgcca aacgaacgca aacgaacggc atttaattct gtgtctcgaa
      361 ataaaattgg ttctcttgtc ccccatttga gatttgcaaa tagttgtttg cctacaaaaa
      421 ctacttctgc tctgaatggt gaattttcac cgtaaccaaa agaagtaacc cacttgccga
      481 caacaggaac attttgtgtt tttaaaatgt gtcttcctgc accaaaaaca tcaagtgctt
      541 ttccatctct gaaaaaaact gcttcttgac tttctctaac tgtcagttgt gaaccccaga
      601 ttatttctag gtgtccacta tcgggaatcc gttttactaa aacagttccg ctgtcatcta
      661 caaattcaat tacatccatg tctatttaaa ttggtttatg aaatttgttc gtttaattaa
      721 tattgcatca atttctctga cttgctgatt tacaaaacca gcactcattg tttcgtttat
      781 ttcttcttca actgttttta aaattaaatg aactctttcc gacatttcta aatctatttt
      841 gtaaatctca tcaagttctg tttcttttaa ttgttggtcg ctaaaaaaag aggattcacc
      901 gtaagtagca tttttgattt ttgaaaaaat tgtgttcaac gctttccttg ctatttccca
      961 ttcttgacac aattgcattt ctcctgtttt tagaagttgc tgttggtgtg tgataacggt
     1021 cttctcggat tgttgaatta ttcctgaaag ttcgtcacgc aattttttgt cggtgtttcg
     1081 cttttcgtca cgaagagagt aacccttgta accacgaatc atgtttccga ttttttcaaa
     1141 aagatttgtt cgccttgttg tcataagttc ttggttaatt taaaagttac tgcaatatag
     1201 tttaagttgg gaatttcgat tgtgtggtcg ggcaaaagca aatgtgctgc aattgtgtcg
     1261 gcttgcggtg ggcattgcag ctcttttggt tttgctgaag cgttggcttg tttgttcatt
     1321 tgtctatctg tttaatgtgt gcactgtcgt tcatagggtg accgctaact tgtttattgc
     1381 cgcaacccca tttcgcctaa caccccaaac cggaagctaa agcgaaacaa aagttaattt
     1441 acaagcaatt tgctatctac aaatttacac ttccggacgg attgaaggtt aacggaaaac
     1501 cgtgaatgtg aatatgtcac aaaaatggat gaaatttaag gtgtaaatgc ccgacagaaa
     1561 tggttttcag ctgcattttg gacaaatatt ggtgcatttg tggtgtcaca ttttggggaa
     1621 tagcctgtaa gtatgtttgc cgcctttgta gcagctcgcc cacgtgggtc gcagtggttt
     1681 tatttgccgg tctgtgcttt gatcaggaca atccggcaat tttcccggaa atgcttttgt
     1741 ttaatgtggc tttaggtgtc cggttcggaa atatatatca acgcatcctg tccatcgact
     1801 tcactagatc ttcatctttt ttgatgttgt agcgagcgag gaaggaggtg atcataatga
     1861 cagcgggaat gccgaagata aatgctagtc ggaggttttc tccatcggtt ttcatatagg
     1921 ccagggctgc catgactgcc gcgagcaaca tggtggtagc acaaaaacgc atctgcaccc
     1981 ggcggtcttt atacatggca atgataaatc cctgcaataa cgcaattcca acagctactg
     2041 ctgatgcggc tacattatcc tttcctgtta agccatctgc tatatcaaca aacaaaaaag
     2101 caattgcggc tattgctgcc agtgccagaa atatagattg aatgcgttgc cacatagtaa
     2161 actaatttcc tgcaaagata ggcatcagat atcattctat tatttccact acaggcgcct
     2221 acactgcccg gtcatactca taaaaattga gatgacagat actcaattcg aaattgaaat
     2281 caatttgcca atatttccac ttggcgtaac gagcttggcc gtatacattc ctgaaggaac
     2341 agatacctct aatatatttt gctgctgcgt tacaattgtt tccgatacca attgacctgt
     2401 catgttataa ataaataaac gagatggctc tgtcactact tcatcgaaat aaatatatat
     2461 acgatttgca ttagcataca ttgaaacagc ttcatcattc tctgtcatgt cttctccctc
     2521 taacctgcat gatgccgttg tgaaagtttg cacctctgaa tagtcggaca acgaaccatc
     2581 caaacaagcg caacgcacct gccattcata atctgtacta cattccaatc catttagttt
     2641 agtacttgtt ttatcaggcg gaactgtttt agtttcccat gcccctccgg gtatccggta
     2701 agacacttcg taatgatcta tatcaacacg tggctcccaa tatactttag cccaatgtga
     2761 agcagttgtg ccggttccta ctgcatctgt agcaatacaa attaatcctt cacccgccaa
     2821 tatcggcgta tttggcgtcc agttgatggc atagtcataa atcttaatgg aggttggtac
     2881 cggaacaaac ggcgtgcttt tatgaattga aagccgcatc cagccataat atttcaacgg
     2941 accatttttt aatctgaaac caatgtattt aaccactgct gatggcattc ccgctgtcca
     3001 acaataatcc atcatgctag aatacatggc agggaagcac tgctgataaa ataattcaac
     3061 attattgttg aaattcaagg tattgttaat aggtgtattt gcaggtaaaa tttgcgcaaa
     3121 ttttgcacta agatttgcaa ataatgcact tgaccatgta taaatattag tcgattgcat
     3181 ggtgtatgca attctggcac cattaatacc atctaccccc agatgatcgt aatttacagc
     3241 agatgctgta tatgatgtat aaaaataaaa ggcattaaaa atgaaatccg taattgcatc
     3301 accggtaaca tcaagatgca gcgtgtcata cggacctgta ctttgcaaaa ctttgtcggg
     3361 attgatattc gtgtagatga cctgaccatc agcttcattc tgaatggcta atacagttgc
     3421 agccattgta gaataagctg caagtcgcga aggggtagtt gcaattttca tattgagttt
     3481 tattgcaaat ttaatataat aaaactcaac ttccacttac ataaaatagt gaaacgcgca
     3541 caatggccat aaaatgggat aacattagat aaatttatat gctatttgga ctgaattagc
     3601 gtcaatttta tgtctatagg agggttaatt ccaataaaac gcctatttaa acttgattcc
     3661 ccgactaaga aatacagctc agattgcaac tcaccggcaa gaagaaacaa gaggagatat
     3721 tcagcagctc gtttaaggtc atataaatga ccatcacaca catttctcct acctttgcca
     3781 catgcgctac tttgcagaaa tagcttatcg cggtacacat tatgttggct ggcagataca
     3841 gccgaatggc gtcagcgtgc agcagaaggt caatgaagcc ctcagaaaaa ttctgcgcga
     3901 tgaagaaatt gcctgtgttg gctgcggtcg cacagatgcc ggcgttcatg ctactcagtt
     3961 ttttctgcat tatgatacgt tcgccgaaat acccgaaact tttatgctgc gtatgaatac
     4021 cgtgctcccc gacgatattg tggtaaaaag ccatttccct gtcgccgaaa atgctcatgc
     4081 ccgcttcgat gcaaccctta gagcttatga atatttcatc tccttcaata aagatgcctt
     4141 tggtcccgac agagtcttat ggcggcattt tcgcgacctc gatttcgacc tgttgcaggc
     4201 ttgttgcgag gtgatgaaaa agtataacga cttcccttct ttctgcaaaa gcaaacaggg
     4261 ctcgaaaaca actattatca atctgcagga aatctattgg cgacaagaag gtgatacata
     4321 cattttattc atcagtgccg acagatttat ccgcggtatg gtacgattgg ttgtaggggc
     4381 gatgatgcag gtagcccgcg gaaaaatgac aattgaagaa tttgaatcgg gtatccaaaa
     4441 aggtgaacgc ctgaagctag ctttaagcgc tccggctcac ggattgtatt tgatgagggt
     4501 ggagtatcca taccaattcg gcacattgcc tgagtgaaaa tagttccata aaggcctgaa
     4561 cactagccct tggccaaata aatttgggat tgtaagacat ttgtcctatt tttacagaca
     4621 aatgtctata taccatggct gccgtaaagc aaactttgga aaaaaaactt gataaagaaa
     4681 tcggtattta ctttcaaact accgtgacta aagagttcaa attcgatgac aaaaagagga
     4741 tcacatacga ggattttctt tcagacagga tgttgattat agccgcaatc agagtaggaa
     4801 taccctattc actatttgaa ttaattcaaa tctacacccc tttttcagaa aacgattggg
     4861 ctgatcttct ggatgtttcg acaaaatctt tacagcgata tcgccaagcc tcacaacatt
     4921 attttaagtc atttcattct gaaaaaatta ttgaaatggc agaggtcact aaattaggtc
     4981 tggaagtttt cgaagatatg gacaaattaa agctatggct taacactcca aattttgcgt
     5041 tgggaaaaat caaacccatc gagttattaa aagactctta cggcaaagat ttagttacct
     5101 cggaattggt aagaattaat cacggaattc tggtgtaagt tgtggaggta tttagattag
     5161 cacgagagaa atatgctcat gcgctctccg gaaagggatc agctattaaa ggtgcgcgat
     5221 ggaattcagt cggtgttgaa ctaatttata ccgcctcgaa caggtcgttg gcaatggctg
     5281 aagtggccgt gcacctcaca ctcgctacat tgcccggcga ttttaggatg tttactattc
     5341 atatccctga cactatcgcc attaaggaag tcaaaatgaa atatcttcca accaactggc
     5401 aggaatttcc acatcctgcc actacacaac gtttcggtga tgagttcgta gcggctaata
     5461 aatattgtgt gttaaaaata ccttcggtag tgacacaagg agattttaat tacctcatca
     5521 acccttacca tgcagatttc aagaaaataa aaatcattga aatagtaaat ttcccattcg
     5581 ataaaagaat agttaaatag gattttactg caattacttt tctacaatcg ccaacttcgc
     5641 aaacttcaac agcaatttct tctgccccgc tttatggaaa ttaattgtcg ccattaagcc
     5701 atcattctga ccttctaaag ctgagattgt tccaaatccg aatttctgat gctccacttc
     5761 catgccggct tttaattctt ccggaaaagc cactttgaat cctgctgaag gtgcatgcac
     5821 atagttttga accggggcta caggcttggc tttcgctatc ggtgtggcat gctcatatgc
     5881 cttgtcgaaa atatttcttt catcctgccg cgatgtctta ttgtaaccaa catatgtgag
     5941 cagattttgt ggtatctctt ccagaaatct actcggttcg caatattcca gattgccata
     6001 tttatatcgc gaagcagcat gtgtcaacac cagtttcttt tctgcccttg tgaccgctac
     6061 ataaaataat cgtcgttctt cttccagctc atcgcgagag tttacagaca atgcacttgg
     6121 aaataaattc tcttccaacc caacaacaaa tacactctta aactccaatc cttttgccgc
     6181 gtgtatggtc atcagtttca cggtttcatc ttcattccct tcctgatcgg catcggtgag
     6241 caaaactact tgttgcaaat attctgctaa gctgcgagga cctgtatttt ccgttggtaa
     6301 tgtttctgtg aaataaggag aatttgcttc atcaggtgag gcaaattctt tgatggagtt
     6361 caacaactcc tgcacgtttt cgtatctgct cagtccttct acactctgat cgttatagag
     6421 ctctttcagt atgcctgtag attttgcaat atgactggct acttcataag catctttcgt
     6481 ttccaacatg gtttgaaaac tcctgatcat ggtaataaaa tcggtaaaca atttccgtgt
     6541 gccggcatta aatggcaata gatcgatatt ctctgccatg gtaaacaagc tcttctcatt
     6601 atctgccgcg aaaacagtta atttttcaaa tgatgttttt ccgataccgc gagcgggata
     6661 attgatcacc cttctaaacg attcttcatc attcggattt actgtcagct tcaaatatgc
     6721 aaccagatcc tttatttcct tgcgctgata gaacgacaat ccaccataaa tacgatacgg
     6781 aatattcaat ctgcgaagtg cctcttcaaa cgagcgactt tgcgcattgg tgcgatacaa
     6841 aatggcaaat tcaccatttt taaaatgatc gcgcatgcgt tgttcgaaaa tctgatcggc
     6901 aacaagtctt ccttcttcat tatcactcat cgcgcgaatt accttgatgg tatcgccttc
     6961 cgtattatct gtccaaatgg tttttgcgag ctggtgtctg tttttactga tgatctgatt
     7021 tgctgcttcc acaatcactt tcgtgctgcg ataattttgt tccagtcgga atgtttgcac
     7081 ctcaggaaaa tctttctcaa aattgaggat attggcaata ttcgctccgc ggaaagcata
     7141 aatactctgt gcatcatcac ccactacggc aatattctga tacacatctg ccagtcggcg
     7201 cacaatagca tattgcaaat gattggtatc ctgaaactca tctatcatca catatttaaa
     7261 gcgatgctga tatttatata atgagtcggg aaaccgctcg aataattcat gtgtcttcaa
     7321 caacagatca tcaaagtcca tcgccccggc tctgaaacat cgcttggcat actgttgata
     7381 cacctccgac attttaggtc gaccgtttgc ttcatcctct gcgataaaat cagtattatt
     7441 tgcgtattct gccggaccaa tgagattatt cttacatagc gaaatgcgat acaaacaata
     7501 acccggttta tacagtttat cgttcaatcc catttccttc acaatattct tcagcagatt
     7561 ttttgcatca tcggtatcat aaatggtgaa attctgcggg tatccgagtt tatctgcctc
     7621 actacgcagg atacgagaaa ataccgagtg aaatgtcccc atccacaaac tccgcgcatc
     7681 accgcccatc actttttcga tgcggttttt catttctcgt gctgccttgt tggtaaatgt
     7741 caacgcaaga atagaaaaag ggtctatccc tgtttccatt aaataagcaa cacgataagt
     7801 aagcacacga gttttccctg accccggccc tgcaatgatc attgttggtc cgctcacatt
     7861 ggtaacggct gcgcgctgcg cctcattcaa ttcatccaaa aaactactca cggagcaaat
     7921 ttaaggaaag gaggggaatg gtgagtggtg aatggtgagt ggtgagtttt ttgagtatga
     7981 ccgactttgc gccttagcc
//
LOCUS       DAOQOU010000151         7992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5024_length_7992_cov_6.975558, whole genome shotgun sequence.
ACCESSION   DAOQOU010000151 DAOQOU010000000
VERSION     DAOQOU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5024_length_7992_cov_6.975558"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1220
                     /locus_tag="PLM90_11625"
     CDS             273..1220
                     /locus_tag="PLM90_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HMZ90073.1"
                     /translation="MNTIPTVNFADFISDDADRKARFVQELGKAYQEVGFVAVQNHGI
                     DQQLVDGFYNAVESFFAMPDDIKSKYEIAGLAGQRGFTSFGKEHAKQSTAGDLKEFWQ
                     IGQTVEDGAALSEHYPENVRITEIAEFNELGYQLYRNFENTGKYLLRSLALFLGLEET
                     YFDKPIHNGNSILRAIYYPPITSEPKSAIRAEQHEDINLITLLVGASADGLEVMDTAG
                     KWIPVKTAPTEIVVNVGDMLQRLTNNVLRSTTHRVVNPPREKWHTARYSIPFFLHPVS
                     EMPLDCLENCVTAHNPLNYSPITAGEYLDERLREIGLKK"
     gene            1326..2135
                     /locus_tag="PLM90_11630"
     CDS             1326..2135
                     /locus_tag="PLM90_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ90074.1"
                     /translation="MKKILTLMCFSAVIGTVSAQQGLHFGAQGGFNSVWIMNQNNYGF
                     QELDYTRTFGYLAGASFGYNWDQTIGFQVDLNYVKVGQHYFDVSKDFCNIDANGDNKP
                     EKVDTYRYVDLNYIQLPIMFKYTSPRSKKQVVSVHILAGPTLGILAGADMYYEADTLA
                     LYNDEVQLIPGDSIDYMVPEFAQTAATEAASDYFSKIDIGFQAEIGADIYVNKNLYIS
                     PGLRVYGGLTDINSKPTRERRPDLETVSYEGASRNIYGGLTLGIHYMLPSK"
     gene            complement(2354..4423)
                     /locus_tag="PLM90_11635"
     CDS             complement(2354..4423)
                     /locus_tag="PLM90_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90075.1"
                     /translation="MKKLLLLTLAALPIVGWSQCTTTNATGCHCLDGSTECDLLPDIT
                     ASYDLLAETDETVETPGELRLSVGTPNIGHGPLRVVATDYYVCGGDTIYSPGGLTTCP
                     DGSSPKQIINQRIYHKSGSDMSYWERAAGTMTYHPDHGHFHTDNWGNYTLRKPVDGVD
                     DPTEWPIVGYGTKMGFCLMDLANCATPSNYGYCREDDGTVITNDIENYGLGGGGYNCV
                     VTNQGISVGYLDIYDYYLDGMSITIPEGVCNGDYYIVVEIDPNLNYWEESDNNNVIAV
                     PITLTEQPETIDLVHIAHSAGTELTTGTLTVCATESVELSVSPIGTDYLWSNGATTST
                     ITITEPGTYSCFITRECGDVYSDTINVEIVTSVEPVIDPSPVVCIGDATTLTATGDGI
                     INWYNAPIGGSMLGTGTTLITAALTENTTFYAENVNEILSAIDAFVGQTNHEGSNYST
                     GSPYNGYEVFDAIEDVTINSVKVYTDYPGDRLIELRNAAGTVMASSLVNIPSGTTVID
                     LDFDVPAGSNYRLGTNDAQNTTTFGDIAPRLKRSTSGTSYPYTVDGLISITNSSFDVT
                     RWYYFYDWHVTGNAVFSCPSERAAMAVEVKECTAIGELSSLNSFSVYPNPANGVFTVD
                     LNTQSVKDLAVSVCNATGQEVYGTVMSYVNGAITIPVDIRNAASGMYLVKVTSEGKSV
                     SKTIIVE"
     assembly_gap    4840..4939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4940..5963)
                     /locus_tag="PLM90_11640"
     CDS             complement(<4940..5963)
                     /locus_tag="PLM90_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HMZ90076.1"
                     /translation="MSEIAQQLTAKDFQTDQEVRWCPGCGDYSILKQVHTVLPQLGIP
                     RENIVVVSGIGCSSRFPYYTNTFGMHSIHGRATAIASGLKAARPELSVWIVTGDGDGL
                     SIGGNHMIHLLRRNFNVNVLLFNNEIYGLTKGQYSPTSPVGKVAKSTPMGSIDHPFNP
                     AALALGADATFVARSMDRDPGHLQEILIRANQHKGTSFTEIYQNCNIFNDGAFEVFTE
                     KATKKENDLFVKNGQPLLFGEGDTKGIKLDGFTPIVVDLNDSHSKDDLWIHNENDIYK
                     AQILVRIFDDPHRDGHMPRPFGVFYENPTRATYEDMMTDQLNAAIAKGPGNLDKLLRG
                     NETWEIK"
     gene            complement(6054..7883)
                     /locus_tag="PLM90_11645"
     CDS             complement(6054..7883)
                     /locus_tag="PLM90_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMZ90077.1"
                     /translation="MNIQTIEDVVIKFAGDSGDGMQLTGGQFTTNTALLGADLATFPD
                     FPAEIRAPIGTLPGVSGFQLHFGSRKIFTPGDLCDVLVAMNVAALKVNIRNLKKGAIV
                     IVNTDGFDQKNLRLANADTNPLEDGTLDGYVVIKIDVTKLTREALSEIQMGVKEKDRC
                     KNMFVLGFLYYMYSRTLDSTERFLNAKFSKKPEVLEANVKALRAGYNYADTTEVFSSR
                     YQVEAAKLEPGTYRSIMGNEAAAMGFVAASQKCGLPLFLGSYPITPASDILHYLSYNK
                     NFGVRTFQAEDEIAAICSSIGASFGGHLALTTTSGPGVALKAEAMGLAVMLEIPLVIV
                     NIQRAGPSTGMPTKTEQADLLQALYGRNGECPMPVIAAARPSDAFDVAFESCRIALNH
                     MLPVMMLSDGYIANGAEPWKFPQSSDLPEIPVKFAEPITDGSVYKPYERDEKLSRPWA
                     IPGTKGLEHRIGGLEKANITGNISYDAENHEYMVKLREEKVERIADYIPLQRIESGPE
                     KGKVLVVGWGSTYGAIQSAVNQLLADGYSVAHAHIRYLKPFPKNLGEILFNYDKVIVP
                     EINNGQLVKVLRDKYMIPAIPFNKIKGTPITSSELIDVIMKNY"
BASE COUNT         2296 a         1670 c         1679 g         2247 t
ORIGIN      
        1 gaattgaaga ttcgaaggtt tgaagaattg aagattcgaa ggtttgaaga attgaagatt
       61 cgaaggtttg aagaattgaa gattcgaagg tttgaagaat tgaggatttg aggtagaagg
      121 ggtcttgaca ttcatagggt ttcgcgcaat gacggctaag aggctaaggc gcaaagtcga
      181 ggtaaactca ccctctcctt tctcctttct cctctctcct ttttcctttc tcctcccaac
      241 atcgtatctt cgaaattata aataccggca gcatgaacac catacccacc gtaaactttg
      301 cagattttat ttcagatgat gccgatcgca aagcgcgatt tgtgcaggag cttggaaaag
      361 cgtatcagga agttggcttt gtcgctgtac aaaatcatgg tatagatcaa caattagttg
      421 atggatttta caatgcagta gagtcctttt tcgctatgcc tgacgacata aaaagcaagt
      481 atgaaattgc gggattagcg ggacaacgcg gattcacgtc atttggtaaa gaacatgcca
      541 aacagagcac agccggcgat ttgaaagaat tctggcagat tggccaaacg gttgaagacg
      601 gggctgcatt gagtgaacat taccctgaaa atgtacgaat tactgaaatt gctgaattta
      661 atgagctggg atatcagtta tatcgcaatt tcgaaaatac cggtaaatac ctattgcggt
      721 cattggcgtt gtttttaggg ttggaagaaa cttattttga caagccaata cacaatggca
      781 actcgatact tcgtgcaata tattatccgc ccattacttc tgagccaaaa tctgccatac
      841 gtgctgagca gcatgaagac attaacctga taacattatt agttggcgcc agtgccgatg
      901 gacttgaggt gatggatacc gcaggaaaat ggattcctgt aaaaacggct cctacagaga
      961 ttgtggtgaa tgttggcgat atgctgcaaa ggctcactaa taatgtccta agatccacta
     1021 cacaccgcgt ggtgaatcct cctcgcgaaa aatggcatac tgcccgttat tccattcctt
     1081 tctttttaca tccggtgtcc gaaatgcccc tcgactgcct ggaaaactgc gttactgcgc
     1141 acaatccgct caattatagc ccaatcacgg caggagaata tctggatgag cgacttcgtg
     1201 aaatcgggct taaaaaatag gtttcacggg atatatcatt ccaagattgc cagtaacacc
     1261 tgttttatgg tgcggatttc tgttaccttt gttgtctata ccctaatatc caaataaaac
     1321 ctgttatgaa aaagatttta accctaatgt gtttcagcgc tgttattggc accgtatctg
     1381 cccaacaagg attgcacttt ggtgcgcagg gcggtttcaa cagtgtatgg atcatgaatc
     1441 agaacaacta cggattccag gaactcgatt acacccgaac tttcggttac cttgccggtg
     1501 cgtcattcgg ttataattgg gatcaaacta tcggttttca agtagaccta aactatgtta
     1561 aagttggaca gcactatttt gacgtgtcta aggacttttg caatatcgat gcaaatggtg
     1621 ataataaacc tgaaaaagtt gacacttatc gatatgttga cttaaattat attcaattgc
     1681 cgataatgtt caaatacaca tcgccaagat caaaaaagca ggttgtgtct gtgcatattc
     1741 ttgcaggacc tacactaggt atactagccg gcgctgatat gtattacgaa gcagatacac
     1801 ttgctttgta taatgatgaa gtgcaattaa taccaggtga ttcaattgat tacatggtac
     1861 ccgaatttgc tcaaacagca gcaaccgaag cagccagtga ttacttttct aaaatcgata
     1921 tcggctttca ggcagaaatt ggcgctgata tttatgtaaa taaaaattta tatatcagtc
     1981 cgggtttacg cgtttatggc ggacttaccg acatcaattc aaaacctaca cgcgaacgtc
     2041 gccctgattt ggaaacggtt agctatgaag gtgcttctcg caatatttat ggtggattaa
     2101 cactaggtat tcactatatg ttgccaagta aataatttaa ataatcatat ttaatgatcc
     2161 cgttgcgttt agtgacggga ttttttttgt gctataatta atttcaaaaa gcgatttaac
     2221 aaacagtaat ctttcaataa taaatcaaag taagcaacta taatatcttg cttcataaaa
     2281 ataccaaagg catcatgcga caaataaaaa agccgcctcg ttatgaagcg gctttcatta
     2341 tttgatattg atattattca acaataattg ttttgctcac gctcttacct tcagaagtaa
     2401 cttttacaag atacattcct gaagcagcat tcctgatatc aacaggaata gtgatggcgc
     2461 catttacgta cgacataact gtaccgtaaa cttcttgacc tgttgcatta caaacagata
     2521 cggcaaggtc ttttacagat tgagtattta aatcaaccgt aaatacgcca tttgccggat
     2581 ttggataaac gctaaagcta ttgagagatg acaactcgcc gatagctgtg cactctttca
     2641 cttcaacagc cattgcagca cgctcacttg ggcaactgaa tactgcattt cctgttacat
     2701 gccaatcgta gaaataatac cagcgtgtta catcgaagct actgtttgtg atcgatatca
     2761 aaccatcaac agtatatgga tatgaagttc ctgaagtact gcgcttcaac ctaggagcga
     2821 tatcgccaaa ggttgttgta ttttgggcgt cgttggtacc taagcgataa ttacttccgg
     2881 caggtacatc aaaatctaaa tcaataactg ttgttcctga aggaatattt acaagacttg
     2941 atgccattac agttcctgct gcattgcgca attcgattaa gcgatctccg ggataatcgg
     3001 tatatacttt aacggagttt atagttacat cttcaatagc atcgaaaact tcataaccat
     3061 tatatggtga acctgtgcta taattagaac cttcatgatt tgtttgtccc acaaaagcat
     3121 ctattgcaga taatatctca tttacatttt cagcatagaa ggtagtattt tctgtcaatg
     3181 cagcagtgat caatgtggtt ccggtgccaa gcatagaacc gccaataggt gcattatacc
     3241 aattaatgat accatctccg gttgcagtta aagttgttgc atcaccgata catacaacag
     3301 gtgatggatc aataacaggt tcaactgatg ttacaatttc aacattaata gtatcggagt
     3361 aaacatcacc acattcacgt gtaatgaagc aagaatatgt tccgggttca gtgatagtaa
     3421 ttgtgcttgt agtagcacca tttgaccata agtaatcagt accgattggt gaaacagata
     3481 actcaactga ttcagtagcg catacagtta aagtgccggt agtaagttca gttccggcag
     3541 aatgtgcgat atgcacaagg tcaattgttt caggttgttc agttaatgta ataggcacag
     3601 caattacgtt attgttatca ctttcttccc aataattgag gttgggatca atttcaacaa
     3661 caatataata atcgccatta caaacacctt cgggaatggt aatgctcata ccatcgaggt
     3721 aataatcata aatatcgaga tagcctacag agataccttg attggtaact acgcagttat
     3781 atccacctcc accgaggcca taattttcaa tatcatttgt aataaccgtg ccatcatctt
     3841 cgcgacaata gccatagttt gatggagtag cacagttggc gagatccatc agacagaatc
     3901 ccattttggt accataacca acaattggcc attctgtggg atcatctaca ccgtcgacag
     3961 gtttacgcaa tgtataatta ccccagttat cggtatgaaa gtgcccgtgg tcagggtgat
     4021 aggtcatagt accggcagcg cgctcccagt atgacatatc agatcctgat ttatggtaaa
     4081 tacgctgatt gatgatctgt tttggagagg atccatcagg gcaagtggtt agcccccccg
     4141 gtgagtaaat ggtatcacca ccacaaacat aataatcggt ggcaaccaca cgcaaaggac
     4201 catgtccgat gtttggagtt ccaactgaaa ggcgcaattc tccgggggtt tcaactgttt
     4261 cgtcagtctc tgcaagcaga tcgtaagatg cagtaatgtc cggtaaaagg tcgcattcgg
     4321 ttgaaccatc aaggcagtgg caacctgttg catttgttgt ggtacactgg ctccagccga
     4381 cgataggcag agcggccaag gtgagaagta gtaatttttt cataataagt tgtctaagcc
     4441 cctttatgcc aaagggatat taaagataaa aatatctttt gaaatgtggg ggggggtgag
     4501 tgggtgagtg gtgaatggtg aatggtgaat ggcctcaccc cggcccgctc ccagagagag
     4561 agggagtgaa tttcagaaat catgtaaatt attctactgt aacgaagtta cagtgccaac
     4621 cgggctagtc ccactactca ctaccagtcg tgaatcgtga gtcgtgactc gtgaattttt
     4681 acctgatacc tgatacctga tacctgatac taaatactgc cagtgggttg gaggttgaag
     4741 gttgtataaa ctcacccact gacccactca cccacttacc acttacttaa tctcccaagt
     4801 ctcgttgcct ctgaggagct tgtcgagatt gcccgggccn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnna cttaatctcc caagtctcgt tgcctctgag gagcttgtcg
     4981 agattgcccg ggcctttagc tatagcggca ttgagctgat cggtcatcat gtcttcgtag
     5041 gttgcacgag tcgggttttc gtagaataca ccgaaaggac gcggcatatg accatcacga
     5101 tgcggatcat caaaaatgcg aaccagaatc tgtgctttat agatatcatt ttcattgtgg
     5161 atccaaagat catcttttga atgactatca ttcagatcaa caacaattgg tgtaaatcca
     5221 tcgagtttaa tacccttggt atcaccttcg ccaaacaata atggttgacc attttttaca
     5281 aagaggtcat tttctttttt agttgctttt tcagtaaata cttcaaatgc accatcattg
     5341 aaaatattac agttttgata tatctcggtg aaggaagtac ccttatgttg attggcacga
     5401 attaaaattt cctgcaaatg tcccggatcg cgatccatac ttcttgcaac aaaagtcgca
     5461 tcggcaccca atgcaagagc agcgggatta aacggatgat cgatagaacc cataggtgtt
     5521 gatttagcaa cttttcctac gggtgaagtt ggagaatatt gtcctttcgt aagaccatag
     5581 atctcgttgt tgaacaataa tacattcaca ttaaaattac gacgaagtaa atgtatcatg
     5641 tgattaccac caatagataa tccatcgcca tcaccggtaa cgatccacac acttaattca
     5701 gggcgcgcag ctttcaatcc ggaggcaatt gcggtagcgc gtccgtgaat actatgcata
     5761 ccaaaagtgt tggtatagta agggaaacgg ctgctgcaac cgataccgga aacaaccaca
     5821 atattttcgc gtggaatccc aagttgtggt aatacagtat gtacttgttt caggatagaa
     5881 taatcaccac accccggaca ccaacgcact tcctggtcgg tttgaaaatc tttcgctgtt
     5941 agttgttgtg ctatttcgct cattgtaatt atatcttatt taatttgcgc ttttcaattt
     6001 ctgcatgctt gcatttttgc atgcttcaat ggtttgtata cgcttaaatg attttaataa
     6061 tttttcatga tcacatcgat cagttctgaa ctggtgatcg gcgtgccttt aattttatta
     6121 aatggaattg ccggtatcat atatttatcg cgcagcactt tcaccagttg tccgttattg
     6181 atctcaggaa caattacttt atcgtaattg aaaagtattt caccaagatt tttagggaaa
     6241 ggtttcaggt agcgaatatg tgcatgagca acagaataac catcggcaag caattgattt
     6301 accgcgctct gaatagcacc gtaagttgat ccccatccaa caaccaaaac ctttcctttt
     6361 tcaggaccgc tttcgattct ttggagcggg atataatcgg cgatacgttc tactttttct
     6421 tcgcgcaatt tcaccatgta ttcatggttt tcggcatcgt aagagatatt tcctgtgata
     6481 ttcgccttct ccaaaccacc aatacggtgt tcgagacctt tagttcccgg aatagcccat
     6541 ggacgactta atttctcatc gcgctcatat ggcttataca ctgaaccatc ggtaataggc
     6601 tcggcaaatt ttaccggaat ttcaggcaga tctgacgatt gtgggaattt ccatggctcg
     6661 gcaccgtttg caatataacc gtcgctcaac atcattaccg gaagcatgtg gttcaatgca
     6721 atacggcaag attcgaatgc cacatcaaat gcatcactag ggcgagcagc agcaattacc
     6781 ggcatagggc attcgccgtt gcgaccgtat aatgcctgca gaagatcggc ctgttctgtt
     6841 tttgtaggca tacctgtcga cggacctgca cgctgaatgt ttacgattac cagcggaatc
     6901 tccaacataa cggcaagacc catcgcttca gctttcagtg ccacaccggg acctgaagta
     6961 gttgtgagcg ccaggtgacc tccgaatgaa gctcctattg atgaacagat ggcagcaatt
     7021 tcatcttccg cctggaaggt gcgcacacca aagtttttat tgtatgaaag gtaatgtagg
     7081 atatccgatg caggtgtaat aggataagaa ccaaggaata atggcagtcc gcatttttgc
     7141 gaagcggcta caaatcccat ggcagcggcc tcattaccca taattgagcg gtaagttccc
     7201 ggctcgagtt tagcggcttc cacctgataa cgacttgaga acacctcagt ggtatcggca
     7261 tagttataac cggctctgag ggcttttacg ttagcttcca atacttccgg cttcttgctg
     7321 aacttggcat tcaggaagcg ttctgtagag tctagtgtgc ggctatacat gtagtagagg
     7381 aacccgagca cgaacatgtt cttgcaacgg tctttctctt tcacacccat ttgaatctcg
     7441 ctcagtgcct ctcttgtcag ttttgtaaca tcgattttga tcacgacgta accatcaagc
     7501 gtaccatctt caagcgggtt ggtatcggca tttgccaaac gaaggttctt ttgatcgaac
     7561 ccatcggtat ttacgataac gatagcacct tttttgaggt tgcggatatt cactttcaat
     7621 gccgccacgt tcatagctac cagcacatca cacaagtccc ccggtgtaaa gatcttacgg
     7681 ctgccaaagt gcaactggaa gccggaaaca cccggcaaag tgcctatagg tgcacggatc
     7741 tcagcgggaa agtcgggaaa ggtagccaaa tcggcaccta aaagggcggt attggtagta
     7801 aactgaccac ctgtgagctg cattccatca ccggagtcac cggcaaattt gatcacaaca
     7861 tcttcaatcg tttggatatt cataataacc gaaatctaaa tttcggcaaa gataaagaaa
     7921 gcccaaatac aaaggggtga tttttaagag aggagaaagg agaaaggaaa gaggagagag
     7981 gaaaaaggag ag
//
LOCUS       DAOQOU010000152         7984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5028_length_7984_cov_6.544835, whole genome shotgun sequence.
ACCESSION   DAOQOU010000152 DAOQOU010000000
VERSION     DAOQOU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_7984_cov_6.544835"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PLM90_11650"
     CDS             <1..1502
                     /locus_tag="PLM90_11650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMZ90078.1"
                     /translation="AYHPLIEFAMWASEPEPFDQMEKQIHQLYAEVASSDLRLQFSMA
                     HEYPLDGTPPIMTHVFFDNNEYKIGCKGGLETLLPLCNADKETIQRVTNQAEIFTGKG
                     FRVLAVAKAAFPENGLPARQNLFSFDLLGLIAFYDPPKAKIKDLIQTFYQAGIQFKMI
                     TGDHAATAISIADIIGMQHNGKILSGNEVMQMSDAALSDAVESTHIYARMFPDAKLRI
                     VEALKAKGEIVAMTGDGVNDAPALKAAHIGVAMGKRGTETAKKAAALILVDDDLMKMA
                     DAVALGRRIYENLKKAIRYIISIHVPIILIVTLPLLLGWAFTDIFQPIHVIFLELIMG
                     PTCSIVYENEPIEKGSMQRKPRKMSMNFFSWQELNLSIIQGLAITIACLGLGYYFMQH
                     QMSDPQVRTAIFSTLLFSNVLLTLTNRSFTHSILHTLKYKNKLIPIILSIAMGILVVS
                     LTIEPVRNLFHFEMLTFSQLITCLCFAIAGVLWIEIYKILKTVNLTAAR"
     gene            complement(1486..3249)
                     /locus_tag="PLM90_11655"
     CDS             complement(1486..3249)
                     /locus_tag="PLM90_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HMZ90079.1"
                     /translation="MEIAIVLTLLVLAIIAFSTEKISVDIVTMTCVLLLVLSGIITYK
                     EAFAPFGSDFIIMLASIFIVTSAIDSSGVLELWTSKLMRRSAGNKMKLIMPMISITGL
                     FSSIMNNTTVTALMINPAMAIAKKSGINFSKILLPLAFASIVGGTCTLIGTSTNVAVN
                     AYLEKNNFPVLGMWDFTWIGVIMLLVTLLYMVLLGVRMLPDRKNDELTDNYGIRSYLS
                     EIKILPNSSLIGQVIQDSDISRMGFTVLAVIRGDRQFAPGENVSFRENDIVLVKGLMQ
                     QLIGVKNTKGIDIVADTLDFYNEKDNKLRLAEMVVPGRSNLAGFTIAESDFRRKYGFV
                     VVAINREGKNITEQLNNIRFEVGDMLLVQGRNEAFDKLSSHHDLVLLGDHTPPVKNLW
                     KGYFALAFFMLAIVVSSIPAFNVHVSVAFLASAVLSMLVGAVKPEDAYKNIEWKLLVL
                     IAGMTAFGTAMANSGADKYLAGLIVDWFGGMGPMGIMAGFMVLTVLLTQPMSNAAAAL
                     VVLPVALEAAHTLDVNPLTYAVAVMLSASVSLITPFEPSCILVYGPGKYKFGDFFKVG
                     GILTLILMVIIYLTVPITWPL"
     gene            complement(3432..4202)
                     /gene="pstB"
                     /locus_tag="PLM90_11660"
     CDS             complement(3432..4202)
                     /gene="pstB"
                     /locus_tag="PLM90_11660"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HMZ90080.1"
                     /translation="MTSSTIKSHISIKDLNMHYGKNHALKNISINIPDKKLTAIIGPS
                     GCGKTTLLKTFNRLIENVDGTNITGEVLVDGENIYGKNADVTNIRKKMGLLNQRPTPL
                     PMSIYDNIAFGMSIHGIKQKGVLNEKVEHFLRVASLWDEVKDRLHSPAARLSIGQQQR
                     LCLARGLAVEPEIILGDEPTSALDPISTKHIEQKFVELKEQYTIVMVTHILRQARRIA
                     DYVIFMYLGEIIEHGPTEMVLGNPQHQLTKDYVGGAIS"
     gene            complement(4212..5060)
                     /gene="pstA"
                     /locus_tag="PLM90_11665"
     CDS             complement(4212..5060)
                     /gene="pstA"
                     /locus_tag="PLM90_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HMZ90081.1"
                     /translation="MKKRKIEELIFSIIMRVSAFIIFGSLIAISATILIKGVPAMNLD
                     MITKTPSGGFFLGKEGGILNAIIGSFYLSVGATIFALMICIPVVIYINVYASENSKIV
                     HYTRLSLDILYGIPSIVFGAFGFVLMLFLGMHVSLLAGMITVGLLIIPIIARTMDEVM
                     RQVPVELKEASYSLGATKWETASKVLLKQALPGLATASLLGLGRAIGDAASVMFTTGF
                     TDNIPHSLQDPAATLPLAIFFQLSSPIAEVRDRAYAAALILTVIIFAISLISRILIKR
                     FSKNTL"
     gene            complement(5066..6073)
                     /gene="pstC"
                     /locus_tag="PLM90_11670"
     CDS             complement(5066..6073)
                     /gene="pstC"
                     /locus_tag="PLM90_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HMZ90082.1"
                     /translation="MPKEINDDDIQNITENIDELEITAVSTQQSTPLVQTGSNPLRVI
                     LDGFMGKVVKAMTIFSLLLLVLIIIGLVLKSIPVLKDNPLSTLLFSSQWSPFKGEFGF
                     LPFILSTVYVTLIAAVIAIPLSLFTAIYLSEYANKRFLKYAAPMLDILGGIPSVIYGI
                     WGVLLIVPFVRDDFSAWLGVDSVSGYSILSGGVVLAVMVFPVVVQIVYEVLKTVPHEL
                     REATLSLGATKWETIKKVVIKRASPGIIAACILGFSRAFGETIAVLMVVGNVVQIPKG
                     IFDEGYPIPALIANNYGEMMSIPMYDSALMFAALILLVIVLVFNVYARWVLNRIEKSI
                     K"
     gene            complement(6101..7081)
                     /locus_tag="PLM90_11675"
     CDS             complement(6101..7081)
                     /locus_tag="PLM90_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMZ90083.1"
                     /translation="MKPSKLFLLIAISAITFSSCSGDKKENTEEAGKLEGKISISGAF
                     ALYPMAVQWGEEFKKLHPDVQFDIQGGGAGKGMTDVLSGTVDLGMVSRDVNDEEKSKG
                     AFDFSVCKDAVLPTINAENPYRELLYQKGISNQQFIDVFITGKITTWGQLLGNGAKEK
                     IDLYTRSDAAGAAESWAKFLGGKKQEDLKGVGVMGDPGLAQAVVQSKFSLGFNNVSFA
                     YDNVTKINNPGIAVAPIDVNSNGTLDADENFYGNIDQVNEAILNGKYPSPPARPLLFV
                     SKGNPTDPVVIAFLQYVLTEGQKGVSDAGFVALPADVIKQQIEKLPSAKQ"
     gene            complement(7179..>7984)
                     /locus_tag="PLM90_11680"
     CDS             complement(7179..>7984)
                     /locus_tag="PLM90_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMZ90084.1"
                     /translation="KKLHPKVSFDIQGGGAGKGMVDVLAGNVDLGMVSREITKEEAGK
                     GALGFAVCKDAVVATMNANNPYLSKIYETGITRQQFYEIWMGRSITTWGQLLKNGSKE
                     KISVYTRSDAAGAADSWAKYIGGATKKQDDLEGIGIFGDPGLAQAIASDKLGIGYNNV
                     GYAYDFTTKKPISGIAIVPIDLNENGKLDPEENFYSNADKLNMAVLSGKYPSPPARNL
                     YFVSKGEVTDPVLKEFLKWVLTDGQKYVAESGFVVLPKQTLEEQVAKIK"
BASE COUNT         2425 a         1730 c         1470 g         2359 t
ORIGIN      
        1 ctgcatatca tccgttaata gaatttgcta tgtgggcttc agagccggag ccgtttgatc
       61 agatggaaaa gcaaatccat caactgtatg cagaagtggc atcaagcgac ttgcgattgc
      121 aattttcgat ggcgcatgaa tatccattag atggcactcc acccattatg acgcatgtgt
      181 tttttgataa caacgaatat aaaataggtt gtaaaggtgg attagaaaca ctgctgccct
      241 tgtgtaatgc agataaagaa acaatacaac gcgttacaaa tcaagctgaa attttcaccg
      301 gaaaaggttt tcgtgtattg gctgttgcga aggcagcatt tcccgaaaat ggattgcctg
      361 ccagacaaaa tttattcagt tttgatttat tagggttgat agcattttac gaccctccaa
      421 aggcaaaaat taaagacctc attcaaacat tttatcaggc aggcatacaa tttaaaatga
      481 ttaccggcga tcatgccgct actgccattt ccattgctga tatcattggt atgcaacaca
      541 atggaaagat attgagcggc aatgaggtga tgcaaatgtc agacgccgcc ctttcggatg
      601 ctgttgaaag tacgcatatc tatgcacgca tgtttccgga tgcaaaattg cgcattgtgg
      661 aagcacttaa agccaaaggg gaaatagtgg caatgacggg agatggtgta aatgatgcac
      721 ctgcattaaa agctgcacat atcggtgttg ccatgggtaa gcgcggaaca gaaaccgcga
      781 aaaaagcggc agcacttatt ttggtagatg acgacttaat gaaaatggca gatgctgttg
      841 cgttgggtcg aaggatatat gagaatttaa agaaggctat ccgatatatt atcagtatcc
      901 atgtgccgat aattttaatt gttacacttc ctttattgct tggttgggca tttacggata
      961 tcttccaacc gatacacgtc atttttctcg aactgattat ggggcctact tgttctatag
     1021 tctacgaaaa cgaacccata gaaaaaggtt ccatgcagag gaaacccaga aaaatgtcga
     1081 tgaatttttt ctcgtggcaa gaattaaatt tgagtattat acaagggttg gcgatcacta
     1141 ttgcctgcct aggactaggt tattacttca tgcaacatca aatgtcggat ccacaggtgc
     1201 gaactgcaat tttcagtacc ctcctattca gcaatgtgct gttaacattg accaacagat
     1261 cgttcaccca ctccatcctg catacactta aatataaaaa caaattaatt ccaataatat
     1321 tatccattgc aatgggaata ttagttgtgt cgcttacaat agagcctgtg cgcaatttat
     1381 ttcatttcga aatgctgaca ttctcccaat taatcacttg tctatgtttc gcaattgccg
     1441 gtgtactctg gatagagatt tataaaattc tcaaaacagt caatcttaca gcggccaggt
     1501 gattggaacg gtgaggtaaa taatcaccat taaaatcaaa gtcaatattc cacctacttt
     1561 aaaaaagtcg ccgaatttat attttccggg accgtagacc agtatacaag atggttcaaa
     1621 tggcgttatg agtgatacgg aggcggataa cattactgca acagcatatg tcaacggatt
     1681 aacatcaagc gtatgcgctg cttcaagggc taccggcaat acgaccagag ctgcggctgc
     1741 attcgacatg ggttgagtta agagcacagt aagcaccata aatccggcca taatacccat
     1801 aggccccatt ccgccaaacc aatcaacaat taatcctgca agatatttat cggcaccgga
     1861 gtttgccatt gccgtaccga aggctgtcat accggcaatc agcactaata gtttccattc
     1921 aatattctta taggcatctt caggcttcac agcgccaacc agcatgctta atactgcact
     1981 cgccaagaat gcaacagaca catgcacatt aaatgccgga attgagctaa caacaatagc
     2041 tagcataaaa aatgccaatg caaaataacc cttccataaa ttcttgacag gtggtgtgtg
     2101 gtcgccaagt aaaacgagat cgtggtgact gctcaattta tcaaacgctt catttcttcc
     2161 ctgaacaagc agcatgtcgc caacctcaaa tcgtatatta tttaattgtt cggtaatatt
     2221 ttttccttcg cgattaattg ccacaaccac aaaaccatat tttcgtctga aatcgctttc
     2281 ggcgatcgta aaaccggcaa ggttagatcg cccgggaacg accatttcag ccagacgcag
     2341 tttgttgtct ttttcattat agaaatccaa agtatccgca acgatatcaa ttcctttggt
     2401 attttttacc ccaattaatt gctgcatcaa acctttaacc agcacaatat cattttcgcg
     2461 aaaggataca ttttcgccgg gcgcaaattg cctgtcgcca cgaatcacag ccagcactgt
     2521 aaaccccatt ctgctgatat ctgaatcctg tataacctgg ccgatgagtg atgagttagg
     2581 taaaatttta atctcactca aatacgatct gataccataa ttatcagtca attcatcatt
     2641 tttcctgtca ggcaacatcc ttactccgag caataccata taaagaagcg taacgagcaa
     2701 cataataacg cctatccagg taaaatccca catacctaag accggaaaat tatttttttc
     2761 aagataggca tttacggcaa cgtttgtaga ggttcctatt aatgtacagg ttccgccaac
     2821 aatagaggca aaagccagag gcagcagaat ttttgaaaaa ttaattcccg acttcttcgc
     2881 aattgccatt gccggattga tcatgagtgc ggttacggtg gtgttattca ttattgaaga
     2941 aaacaaaccc gtaatagaaa tcattggcat aatgagtttc atcttgttac cggctgatcg
     3001 gcgcatgagt tttgatgtcc ataattccaa aacgcctgac gaatcaatag cactggtcac
     3061 aataaaaatg gatgccagca tgatgataaa gtcgcttccg aaaggggcga aagcttcttt
     3121 ataagttata ataccactta aaacgagcag cagaacgcag gtcatggtga caatatccac
     3181 cgatatcttt tcggttgaga aggcaataat agccagcacc aataaggtga ggacgatggc
     3241 aatttccata atgtggcaat tttagatggt agtgcaaaaa tatgcttttt ctataaagta
     3301 tatgtattta gtagattatt agaacacatt tttatgtttt taaagtataa acaccttgtt
     3361 taagtgaaaa aaggccccgt tcacgcaaac ggagccaaca ttcaaaacca aatttacact
     3421 gtcggaactg atcagcttat ggcgccacca acataatctt tggttagctg gtgttgagga
     3481 ttgccaagta ccatttcagt tggcccgtgt tctattatct cgcctaagta catgaagatg
     3541 acataatctg caatacgtcg ggcttggcga agaatatggg taaccataac aattgtatat
     3601 tgttctttca gttcgacgaa cttctgttca atatgtttgg tagagatagg atcaagtgcg
     3661 cttgtaggct catctccgag aattatttcg ggttcaactg ctagtccacg cgcaaggcac
     3721 aagcgttgct gctgtccaat tgataagcgt gctgcgggcg agtgcagtct atcctttact
     3781 tcatcccata agcttgctac acgtagaaaa tgctccactt tttcgttgag tacacctttt
     3841 tgcttaatac cgtgaatgct cataccaaag gcgatattgt cataaatact cattggaaga
     3901 ggtgtaggac gctgattgag taaacccatt ttctttctga tattcgtcac atcggcattc
     3961 ttcccataga tattttctcc atcaaccaat acctctccgg tgatgtttgt gccatcaaca
     4021 ttttcaatca gtcgattaaa tgttttcaga agcgttgttt taccacatcc acttgggcca
     4081 atgatggctg tgagcttttt atcaggtata ttgattgaaa tatttttcaa cgcatggttc
     4141 ttaccatagt gcatatttaa gtctttgata cttatatgcg attttattgt actagaggtc
     4201 ataaataaat tttagagggt gtttttagaa aatcgcttaa tcaatattct actgatcagg
     4261 cttatggcaa aaatgataac tgtcaaaata agtgcagcag cataggctct atctcttact
     4321 tccgcaattg ggctgcttaa ttgaaaaaat attgcaagcg gtaaggttgc tgccgggtct
     4381 tgaagagaat gtggtatgtt atcagtaaac cctgttgtaa acatcaccga tgccgcatca
     4441 cctattgctc ttccgagtcc aagtaatgat gctgttgcca gtccgggcaa tgcctgcttt
     4501 aacaatacct tacttgctgt ttcccatttt gttgcgccga gactgtatga cgcttccttt
     4561 aactctaccg gaacttgcct catcacttca tccatcgttc ttgcgatgat tgggatgatg
     4621 agaagtccga cagtaatcat cccagccaaa aggctaacat gcataccaag gaacaacatc
     4681 aacacaaatc cgaatgcgcc gaaaacgatg ctgggaatac catacaaaat atcaaggctc
     4741 aaacgcgtat aatgtacaat ttttgaattt tcacttgcat agacgtttat ataaatcaca
     4801 acaggaatgc aaatcattaa tgcaaaaatg gtagcaccaa cagataaata aaatgagcct
     4861 ataatagcat taaggatacc accttctttt cctaagaaaa acccacctga aggtgtcttg
     4921 gtaatcatat ccaaattcat ggcaggcaca cccttaatga ggatggtagc agaaatcgca
     4981 atcaagctac cgaatataat gaatgcggaa acccgcataa tgatgctaaa tatcaattct
     5041 tcgatcttac gctttttcat acctactatt ttatactttt ttcaattctg ttcaataccc
     5101 agcgcgcata aacattaaac actaaaacta ttaccaacaa tatgagtgct gcaaacatca
     5161 gcgctgaatc atacatggga atgctcatca tttcgccata attattggca attaatgcag
     5221 gtatcgggta tccttcgtcg aaaatgcctt taggaatttg aacaacattt cctactacca
     5281 ttaaaacagc gatagtttcg ccaaaagctc ttgaaaatcc gagtatacat gccgctatta
     5341 ttccgggtga tgctcgttta ataactactt tttttattgt ttcccacttt gtagcaccaa
     5401 gcgacaaggt agcttctcgc aattcatgag gaacagtttt caatacctca taaacaattt
     5461 gcaccaccac gggaaacacc ataacagcga gcacaacgcc tccgctaagt atggagtagc
     5521 cacttacaga atcgactccc agccaagcgc taaaatcgtc cctaacaaaa ggaacaatca
     5581 aaagcacccc ccaaatgccg taaataacag atggaatacc acccaggata tcaagcattg
     5641 gcgccgcata ttttaagaat cgcttgttgg cgtattcgct caaataaatt gcagtgaata
     5701 aactaagtgg aatagcgata actgccgcta ttaaggttac gtaaaccgta gataaaataa
     5761 atggcaaaaa accaaattcg cctttaaagg ggctccattg ggaagagaac aatagtgtac
     5821 ttaacgggtt atctttcaaa acaggtatcg acttcaatac taaccctata attatcaata
     5881 ccaataataa aagggagaag atggtcattg ccttaaccac cttccccata aacccatcta
     5941 aaataaccct taacgggttc gaacctgttt gtactaaggg tgtgctttgc tgtgtggaga
     6001 cagcagtaat ttctaactca tcgatatttt cagttatatt ttgaatatcg tcatcattaa
     6061 tttctttagg catggcaatg tcatttagat gagtgattaa ttattgttta gctgaaggaa
     6121 gtttctctat ttgctgtttg ataacatcag caggtagagc aacaaaccct gcatcagaaa
     6181 cacctttttg gccttctgtt aatacatatt gcagaaatgc gattacaaca ggatcagtag
     6241 gattgccttt tgaaacaaat aataaaggtc tggcaggtgg tgaagggtat tttccattta
     6301 atatagcctc atttacctga tcaatattgc cataaaaatt ttcatctgca tcaagtgtac
     6361 cattgctatt aacatctatt ggagcaacag caataccggg gttatttatt tttgtcacat
     6421 tatcatatgc gaaacttaca ttgttgaatc caagcgaaaa cttactttga accactgcct
     6481 gagccagacc cggatctccc atcacaccaa ctccttttaa atcttcttgc tttttaccgc
     6541 caagaaattt tgcccatgat tcagccgcgc cggcagcatc gcttctggta tacaggtcaa
     6601 ttttctcttt agctccattg ccaagcaatt ggccccatgt tgtgatttta cctgttataa
     6661 atacatctat aaattgctga ttagatatcc ccttttgata aagtagctcg cgatagggat
     6721 tttccgcatt tatggtagga agtactgcat ctttacatac cgaaaaatca aatgcgccct
     6781 tagatttttc ttcatcattt acatcacgtg ataccatacc taagtctaca gtaccgctta
     6841 atacatcagt catacctttg ccggctcccc caccttgaat gtcgaattga acatcggggt
     6901 gtaatttttt aaattcttct ccccattgca ccgccatagg gtataatgca aaggctccgg
     6961 aaatcgaaat tttcccttca agcttcccgg cttcttcagt gttttccttt ttatcaccgg
     7021 agcaggaaga aaatgtaatt gctgaaatgg caatcaataa aaacaattta gatggtttca
     7081 tatactttat tgttgttttg tttttttata aaagcactat caaacctgct ataaagcagg
     7141 ctgatagtgt tatagttgac cttaaccaat acgtcagttt atttaatttt tgcaacttgt
     7201 tcttcaagtg tttgcttagg caacactaca aatccacttt cagctacata cttctgccca
     7261 tcggtgagca cccattttaa aaactccttt aatacaggat ctgttacttc gccttttgaa
     7321 acaaaatata aatttctagc cggtggtgat ggatactttc cgctcaaaac agccatgttt
     7381 aatttgtcgg cattcgaata aaaattttcc tcagggtcca attttccatt ctcattcaga
     7441 tcaattggaa caatagcaat accacttatt ggtttcttag tagtaaaatc ataggcatat
     7501 ccaacattgt tgtatccaat tcctagctta tcactggcaa tagcctgtgc taaaccagga
     7561 tctccgaaaa taccaatgcc ttcaaggtca tcctgctttt tagtagcacc cccgatatac
     7621 tttgcccatg aatcagcggc tccggctgca tcgcttctgg tataaacaga aatcttttct
     7681 ttactgccat tttttaataa ttgcccccag gtggtaatac tgcgccccat ccatatttcg
     7741 tagaattgct ggcgtgtaat acctgtttca tagattttac tcaagtatgg attattggcg
     7801 ttcatggttg ccacaacggc atctttacac actgcaaagc caagggcacc tttacccgct
     7861 tcttcctttg tgatttcgcg actcaccata cctaaatcaa catttcctgc taaaacatct
     7921 accattcctt tacctgctcc cccgccttga atatcgaagc ttaccttagg atgcagtttc
     7981 ttaa
//
LOCUS       DAOQOU010000153         7905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5091_length_7905_cov_4.742803, whole genome shotgun sequence.
ACCESSION   DAOQOU010000153 DAOQOU010000000
VERSION     DAOQOU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5091_length_7905_cov_4.742803"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLM90_11685"
     CDS             complement(<1..213)
                     /locus_tag="PLM90_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90085.1"
                     /translation="MKTLKSLGIMLLMLCSTALKAQISVEVSEAVSPMSLGERTGYAV
                     TIPRNTEKNAAKAFRAWVTEKQKKAEI"
     gene            376..1764
                     /locus_tag="PLM90_11690"
     CDS             376..1764
                     /locus_tag="PLM90_11690"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HMZ90086.1"
                     /translation="MMKKLFALALMSTTIALVYAQSEETSFSATGRGGTATSFVTDYQ
                     AIGINPANLGFETDNAFSLGVAEFGYGLFSNALVKDDVRAILFNNEDSLTSEDQVTLA
                     RSFLNEGITMNVDVMPIGFSLRLPKLGTFGFSIKGNMAYHTLFAGQAANIVFEGYNYE
                     DYIDTIIFDGSTIYGVAYEPLNLGELFDDTEISLNITSEFNVAYGRKLFGSTDGVSVY
                     GGFGYKYILGLAYLDINSNDGTLNGTAALGLDIMDLQGSDTPFDITTSPYEPVGHGNG
                     FDFGLSVKVHDGLTLGASIVDIGKMTYTANVLQINDFTLDTIAFSGLTSTDPVQLISD
                     ILGDPELIAYSGIESFTVSLPTKLRLGGSIKISDMLNVGVDAVFPMNDVAGAYTTPVI
                     GVGGELTVLKVIKLSTGLSAGGGYAYNIPAGIGFDFKIWELGFATRDVLTWFGQSSPT
                     VSFAAGVLRFKL"
     gene            complement(1843..2703)
                     /locus_tag="PLM90_11695"
     CDS             complement(1843..2703)
                     /locus_tag="PLM90_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMZ90087.1"
                     /translation="MKFAAKVRLRLQKKEKNYLFLPRFRHLSARSAIQGGKWTVCPIF
                     RGKMKLFSSLIAIAMLAGCAAHDSEPKPDLPAEPPPLRQSPLAARMKTAGLVNVQEQI
                     PDILVDLKYSSTDNFVKTDVYGDLDSAYLQPDVVNMLEKAYSRLQTLDSSLTFIIYDA
                     TRPLSVQRHMWEVLKMPIEEKGKYLSNPDKGSIHNYGAAIDISLAVRSTQKALDMGTP
                     FDFFGPEAEPDREAAMLANGKLTQQQFQNRIILRKAMESAGFRQLPTEWWHYNACSRE
                     EAKKRYDVVE"
     gene            2716..3831
                     /gene="atpB"
                     /locus_tag="PLM90_11700"
     CDS             2716..3831
                     /gene="atpB"
                     /locus_tag="PLM90_11700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMZ90088.1"
                     /translation="MKSLIKYALSLLLGWMFLAPMQAKASGGEGEYDAKEFAMHHVAD
                     AHEWHIVSDLYIPIIGRTTINIPLPCIVIHDGLHVFMSNKMPEAHHGAADPAAHAHHE
                     TYQGFYFNESGKIVHEDGGFTLDMSITKNVATLLLVAFIMIWLFTSVAKAYGRREGQA
                     PKGVQSLMEPLILFVRDEVVKPNLGNKSDRYLPYLLTVFFFIWFGNMMGLIPLIANPN
                     LTGNIAVTSALAAMTFLLTNLHANKNYWSHTFNPPGVPLAIKFILVPIEIAGIFIKPV
                     ALMIRLFANITAGHIIILSLVGLIFIFGQAGANLGGGIGGAAVAVPFTLFMSFLELLV
                     AFLQAFIFTMLSALFIALAQEDHGHDHDHAHAEGAHH"
     gene            3867..4109
                     /gene="atpE"
                     /locus_tag="PLM90_11705"
     CDS             3867..4109
                     /gene="atpE"
                     /locus_tag="PLM90_11705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMZ90089.1"
                     /translation="MDLLTIMQAAEGGVKGIAAIGAGLAAIGAGIGVGRIGGSALESM
                     ARQPEVLGEIRTNMIIAAALVEGVALFAVVVALLQG"
     gene            4202..4711
                     /gene="atpF"
                     /locus_tag="PLM90_11710"
     CDS             4202..4711
                     /gene="atpF"
                     /locus_tag="PLM90_11710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMZ90090.1"
                     /translation="MVLLGSFDIITPDPGLFIWTVLVFLILVWILNKYAFGPIKGALK
                     KREESISEALAQAQKAREEMASMKSDNEKILNEAREERSRMLREAKEARDLLINEARE
                     KANAEYNRIVEEAKGAINNQKMAAMIEIKNSVGNMVLEVSEKVLRRELSNKNDQEQYV
                     KQLVDEIKL"
     gene            4751..5311
                     /gene="atpH"
                     /locus_tag="PLM90_11715"
     CDS             4751..5311
                     /gene="atpH"
                     /locus_tag="PLM90_11715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HMZ90091.1"
                     /translation="MSATKLAARYAKSVLDLAKEQGKLELVLSDMRLIQSAIDSNREF
                     YLMLKSPIVHGDKKMEVVLGVFHNKVNEITESFIGILMRKNRESYLPEIIQAFIEQYN
                     TLHHITPVTVITAQPISEELHNTIINRLKTEAGLMKVELTQEVDPALIGGYVLRWDNK
                     LIDNSIARGLTLLKDEFDNNDYIRKF"
     gene            5352..6935
                     /gene="atpA"
                     /locus_tag="PLM90_11720"
     CDS             5352..6935
                     /gene="atpA"
                     /locus_tag="PLM90_11720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMZ90092.1"
                     /translation="MVDVKPDEISAILRQQLSNFKTSSELEEVGTVLQVGDGIARIYG
                     LNNAKSGELVMFENGIRAIVLNLEEDNVGVVLMGASDAIKEGDTVRRTGRIASIKVGE
                     SMVGRVVNTLGEPIDGKGPLQGTMYEMPLERKAPGVIYRQPVNEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQREFYEAGKPVYCIYVACGQKNSTVAQVAKT
                     LTDHGAMDYTIIVAASASDPAPLQFYAPFAGAAIGEFFRDSGRPALIIYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIINNDSIAAQMNDLPESLKPIVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLENNLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFAKFGSDLDAATMAVLNKGKRNVEVLKQKQY
                     SPLTVEKQIAIIYLGTKGLLGDIPVEQVKAFEDAYLTYLETHHKSTLDLLKAGQFNDE
                     ITSVLEKVGIDMSKQYKVA"
     gene            6946..7833
                     /gene="atpG"
                     /locus_tag="PLM90_11725"
     CDS             6946..7833
                     /gene="atpG"
                     /locus_tag="PLM90_11725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HMZ90093.1"
                     /translation="MSGKLKEVRTRISSVMSTQQITKAMKMVSAAKLRRAQDAILQMR
                     PYALKMGELLSNIAEAAGDDVKIDLAQKREVKNVLLVAITSDKGLCGAFNSNVVKALK
                     RTIEERYAHLSNSNIHVMFIGKKAHDQMKRMPYQLHTEYINLFHQLSFEQVAAATDYI
                     KQGFLKGEYDEVIVAYNKFKNAATQILETEQFLPIQKSAPVAGEKKSRTDYIYDPDKE
                     QMILELVPRILNTTFFRYVLDSNASEHGARMTAMDKASDNANELLKELKISYNRARQA
                     AITTELTEIVSGAAALQGS"
BASE COUNT         2179 a         1583 c         1919 g         2224 t
ORIGIN      
        1 tatttctgct ttcttttgtt tttctgttac ccaggcgcgg aatgcttttg ctgcattttt
       61 ttcggtgttg cgaggaatag taacggcata accggttctt tcgcccaggc tcatgggtga
      121 aactgcttca cttacttcaa cggaaatctg tgctttcagc gcagtggaac acagcattaa
      181 cagcattatt cctaaagatt ttaaggtttt catatcattg attttttagg tgaccgaacc
      241 gcctgtatgc aaaaagtatt ccaatttaat attgaacagg cgcatgcact aatatacagc
      301 atgtatttca aaatgtcggc atgtgtattt ccgcttgaaa tggtttatgt atttttggtg
      361 aaataagcct ctgatatgat gaaaaaactg tttgccctcg ccctgatgag tacgaccatt
      421 gcacttgtgt acgcacaatc tgaagaaacc tcgtttagcg ctaccgggcg tggaggaacg
      481 gctacatcgt ttgttaccga ctatcaggca attggcatca atcccgccaa tctgggattc
      541 gaaactgaca atgctttttc gctgggtgtg gcggaatttg gatacggctt attctcgaat
      601 gcccttgtga aagatgatgt gcgggcaatt ttatttaata atgaagatag tcttacgtcg
      661 gaagatcagg tgacactggc gcgttcattt ctcaatgaag gtatcaccat gaacgttgat
      721 gtgatgccta ttggatttag tctgcgttta ccaaaactcg gcaccttcgg attcagtatc
      781 aaaggcaata tggcatatca caccctgttt gcgggacagg cggcaaatat tgtttttgag
      841 ggatataatt atgaagatta tatcgatacc atcatttttg atggcagcac tatttacgga
      901 gtagcctacg aaccactcaa cctcggcgag ttatttgatg atacggagat atcgctgaat
      961 attacttccg aattcaatgt ggcttatgga agaaaattat tcggcagcac tgatggcgtg
     1021 agtgtatatg gaggatttgg atataaatat attttgggcc tcgcatatct tgatatcaat
     1081 tccaatgatg gcacattgaa cggaacagcg gcactgggtt tggatatcat ggatttgcaa
     1141 ggaagtgata cgccttttga tatcaccaca tcgccatacg aaccagtggg tcatggcaat
     1201 ggatttgatt tcggactcag cgtaaaagtg catgatggtt taaccctggg cgcatcgata
     1261 gttgatatcg gtaaaatgac atatactgca aacgttttgc agatcaacga tttcactttg
     1321 gatacgattg ctttcagcgg attaaccagc actgatcctg tgcaattgat ctccgatata
     1381 ttaggtgatc ctgaattgat tgcatattcc ggtattgaaa gttttacagt aagtctgcct
     1441 actaaattgc gattgggtgg ctcaataaag atctctgata tgttgaatgt gggtgttgat
     1501 gctgtttttc ctatgaatga tgttgcaggt gcatatacca cacctgttat tggtgttgga
     1561 ggagagttga cagtattaaa agtaatcaaa ttatctaccg gtctctcggc aggtggtggt
     1621 tatgcttata atattcctgc gggaattggt tttgatttca agatatggga actcggcttc
     1681 gccacaaggg atgtcctcac ctggttcggc caaagcagcc cgaccgtgtc gtttgcagcg
     1741 ggggttttga ggtttaaatt gtgataacgt tttttttcac gcagaggcgc agagatgcag
     1801 gggtataaat gcaactctgc gcctctgcgt gaaataattc aactattcca ccacatcata
     1861 acgtttcttc gcttcttcac gggagcaggc attataatgc caccactccg ttggcagttg
     1921 cctgaaccct gctgattcca tggctttacg caggatgatg cggttttgga attgctgttg
     1981 ggtgagtttt ccatttgcca gcattgctgc ttccctgtcg ggttcggctt cgggaccgaa
     2041 gaaatcgaat ggagtgccca tgtcgagcgc tttttgggtg gatctcacgg ccagtgagat
     2101 atcgatggcg gcaccataat tatggataga ccctttgtcg gggttggaca ggtatttccc
     2161 tttttcttca atcggcatct tgagtacctc ccacatatgc cgctgaacgc tgagtggcct
     2221 ggtggcgtcg taaatgatga aggtgaggct gctgtcgagg gtttggaggc gggagtaggc
     2281 cttttcgagc atattcacca catcgggttg gaggtaggca gagtctagat cgccatagac
     2341 atcggttttc acgaaattgt cggtggaact gtatttgagg tctacaagga tatcggggat
     2401 ctgttcttgc acgttgacga gcccggcggt tttcattcgc gctgccagcg gcgactgacg
     2461 gagcggagga ggttctgcgg gaaggtcggg cttcggctca ctatcatgtg ctgcacaacc
     2521 ggcaagcatg gcaatggcga tcaggctgct gaaaagcttc attttccccc taaaaatagg
     2581 gcaaacagtc catttacccc cttgaattgc gctacgggca gataaatgcc taaaacgagg
     2641 taaaaacagg tagttttttt cttttttttg gagccgaagc cttacctttg cggcgaattt
     2701 caaaatgtta ctgttgtgaa gtcactcata aaatatgcac tttcgctcct gttgggatgg
     2761 atgtttttag cgcctatgca ggctaaagca tcaggtggag aaggcgaata tgacgctaaa
     2821 gagtttgcga tgcaccacgt ggctgatgcg cacgagtggc atattgtttc cgatttatac
     2881 atacctatta tcgggaggac taccattaat attccactgc catgtatagt aattcatgat
     2941 ggattacatg tgttcatgag caataaaatg cctgaagcgc atcatggcgc agcggatcct
     3001 gcggcacatg ctcaccatga aacttaccaa ggtttttact tcaacgaaag cggcaagatc
     3061 gtgcatgagg atggcggttt tacactggat atgtcgatta ccaaaaacgt ggctacgctt
     3121 ttgttggttg ccttcattat gatctggttg tttaccagtg ttgcgaaagc gtatggtcgc
     3181 agggaaggac aagcgcctaa aggtgttcag agcctcatgg aaccactcat tttgtttgtg
     3241 cgtgatgagg tggtgaagcc taacctcggc aataaatctg atcgttattt accgtatttg
     3301 cttaccgtat tcttttttat atggtttgga aacatgatgg gattgattcc gctgattgcc
     3361 aaccctaacc tgacgggaaa tattgcggtt acgtcggctc ttgcagcgat gactttcctg
     3421 ctcaccaatt tgcatgcgaa taaaaattac tggtcgcata catttaaccc tccgggtgtt
     3481 ccgctggcga ttaaatttat tttggtgccg attgagatag cgggtatttt tattaagcct
     3541 gtagctttga tgattcgttt atttgccaac atcactgcag gtcacattat cattttaagt
     3601 ttggtgggac tgatctttat tttcggtcag gccggagcaa accttggcgg tggaatcgga
     3661 ggtgcagctg ttgcggtgcc atttaccttg tttatgagtt tccttgaatt acttgttgca
     3721 ttcctgcagg cgttcatttt tacaatgctg agtgcgttgt ttattgcgtt ggcgcaggaa
     3781 gatcacggtc acgatcatga tcatgcacat gcagagggag cacatcatta attcgtattt
     3841 atttcatcaa ttataaaaac agtaacatgg atttattaac tatcatgcag gcagctgaag
     3901 gcggcgttaa aggtatcgca gctatcggtg caggtttagc agctatcggt gcaggtatcg
     3961 gtgtaggccg tatcggtggt agcgcgctcg aatcaatggc tcgtcaaccc gaagttctcg
     4021 gcgagatcag aaccaacatg atcattgcag ccgctctcgt ggaaggtgtt gccctcttcg
     4081 cagtggttgt tgcccttctg caagggtaaa aagtacaaga tcagtggcaa cggttggttg
     4141 ctgctgatct tcattcattg acattaagac ggtattgccg taaacaataa aaagaataaa
     4201 catggtattg ctcggttcat tcgacattat tactccggac ccggggttat ttatctggac
     4261 ggtattggtg ttcctcattt tggtgtggat actgaacaaa tatgcttttg gtcctatcaa
     4321 aggcgctttg aaaaaacgtg aagaaagcat ttcagaagca cttgctcaag cacaaaaagc
     4381 gcgcgaagaa atggcaagca tgaagtcgga taatgaaaaa attctgaacg aagcacgtga
     4441 agaacgcagc agaatgttgc gcgaagcaaa agaagcacgc gacctgctca tcaacgaagc
     4501 acgtgaaaaa gcaaatgccg aatacaatcg tattgttgaa gaagcgaagg gtgccatcaa
     4561 caaccagaaa atggctgcta tgatagaaat caaaaacagc gtaggtaata tggtgttgga
     4621 agtgagtgaa aaagtattgc gtcgcgagtt gtcgaataaa aacgatcagg agcaatatgt
     4681 gaagcaactg gtagatgaaa taaaattgtg atcatccgtt tcaaattaat cgacgggtaa
     4741 attataacag atgtcagcaa ccaaattagc agcgcgttac gccaaatcag ttctcgatct
     4801 tgctaaagaa caaggcaagc tggaattggt attgtcggat atgcgactga tacaatcggc
     4861 tatcgacagc aacagagaat tttatctgat gctgaaaagc ccaatcgtgc atggcgataa
     4921 aaagatggaa gtggtactgg gtgtctttca caataaggtg aatgaaatca cagaaagctt
     4981 cattggtatt ttgatgcgta aaaaccgcga atcatattta ccggagatca ttcaggcttt
     5041 tatagagcaa tataatacgc tgcaccatat cacacccgtt acagtgatca ctgcgcagcc
     5101 aatttctgaa gagttacaca acactatcat caatcgtttg aaaacagagg ctggtttgat
     5161 gaaggtagaa ttaacgcaag aagtagatcc tgcattaata ggtggttacg tattgcgttg
     5221 ggataataaa ttgatagata attcaatagc acgcggatta acgctgttga aagatgaatt
     5281 tgataacaac gattatatcc gtaagtttta attaattaaa agtattcatt cacaatatat
     5341 aattcagtca gatggtagac gtaaaacctg atgaaataag cgcgatactt cgccagcaat
     5401 taagcaactt caaaaccagc tcagagttgg aagaagtggg aaccgtattg caggtgggcg
     5461 atggtattgc ccgcatttat ggattgaaca acgccaaatc gggcgagctc gtgatgttcg
     5521 aaaacggcat ccgtgccatc gtacttaacc tcgaagaaga caacgtgggt gtggtattga
     5581 tgggtgcatc cgatgcgatt aaagaaggtg ataccgttcg tcgtacgggc cgtatcgcat
     5641 ctatcaaagt tggcgaaagt atggtcggtc gcgtggtaaa tacactcggc gagcctatcg
     5701 atggcaaagg accactgcaa ggcaccatgt atgaaatgcc actcgagcgc aaagctcctg
     5761 gggtaattta tcgtcagccg gttaacgaac cactgcaaac aggtatcaaa gcgatcgacg
     5821 cgatgattcc aatcggtcgt ggtcagcgcg agttgatcat cggcgaccgt cagacaggta
     5881 aaacggctat cgctatcgat accatcatta atcaacgcga attttatgaa gccggtaaac
     5941 cggtatattg tatatatgta gcttgcggac agaaaaactc taccgttgcg caggttgcaa
     6001 aaacattgac cgatcacggt gcgatggatt acacgatcat tgttgcagct tctgcatctg
     6061 atccggctcc attgcaattc tacgcaccat ttgcaggtgc tgctatcggc gaattcttcc
     6121 gcgattcagg acgtccggca ttgatcattt acgatgatct ttctaaacaa gcggtttctt
     6181 atcgcgaggt gtcactgttg cttcgtcgcc caccgggacg tgaagcttat ccgggagatg
     6241 tattctactt gcactcacgt ttattagagc gtgctgcgaa gatcatcaac aacgatagca
     6301 ttgctgcaca gatgaacgat cttcctgaat cattgaagcc tatcgtaaaa ggtggtggtt
     6361 cacttactgc attgcctatc atcgaaacac aagcgggtga cgtttctgcg tatattccta
     6421 ccaacgtaat ctcgattact gatggtcaga tattcttgga aaataacctg ttcaactcag
     6481 gtgttcgtcc tgcgatcaac gtgggtatct ctgtaagccg cgtgggtggt aacgcacaga
     6541 tcaaatcgat gaaaaaagta gcgggtaccc tgaaactcga tcaggcacag tatcgcgaat
     6601 tggaagcgtt tgctaaattc ggttccgacc tcgatgctgc aacaatggcc gtgttgaata
     6661 aaggtaaacg caacgtggaa gtgttgaagc aaaaacaata ttcaccactt accgttgaga
     6721 aacaaattgc tattatttat ctgggaacaa aaggtttgct tggtgatatt cctgttgaac
     6781 aagtgaaagc atttgaagat gcatatctca cttatttgga aacacatcat aaatcaaccc
     6841 tcgatctttt gaaggcaggt cagttcaacg acgagatcac ttcggtgctc gagaaagttg
     6901 gtatcgatat gagtaaacag tataaagttg cgtaattaaa catacatgtc cggtaaatta
     6961 aaagaagtac gcacgcgcat ttcctcggtg atgagcacac agcaaatcac caaggcgatg
     7021 aagatggtat ctgcggcgaa attgcgtcgc gcacaggatg ccattttaca gatgcgccct
     7081 tacgctctga aaatgggtga attgctgagt aacatagcgg aggctgcagg cgacgatgta
     7141 aaaatcgatc tcgcacaaaa acgcgaagtg aaaaatgtgt tgttggtagc tattacttcc
     7201 gacaagggat tgtgcggtgc ctttaattca aacgtagtga aggcattaaa gcgcactatt
     7261 gaagaacgct atgcgcattt gtcgaatagc aatattcatg tgatgttcat cggtaaaaaa
     7321 gcacatgatc agatgaagcg tatgccttat caattgcata ctgaatatat caatctgttt
     7381 catcaattat cgttcgaaca agtagctgct gcaaccgatt atattaagca aggatttttg
     7441 aaaggtgaat atgacgaggt gattgttgca tacaataaat tcaaaaatgc agcaacgcag
     7501 atattggaaa cagaacagtt tctccctatt caaaaatctg cacctgtagc aggcgagaaa
     7561 aaatcgcgca ccgattatat ctatgatcct gacaaggagc aaatgattct tgaattagtg
     7621 cctagaatat taaatactac cttcttccgt tatgtgctcg acagcaatgc atcagaacac
     7681 ggtgcacgta tgacggctat ggacaaagcg agtgataatg cgaatgagtt attaaaagaa
     7741 ctcaagatca gctacaaccg cgctcgtcag gctgccatca ccaccgagct caccgagatc
     7801 gtgtccggtg ctgctgcttt gcagggaagt tgatttcatt gaagatatat tttttttgaa
     7861 gactcatcgt tttgatgggt cttttttttg gtgagcctgc ctcgc
//
LOCUS       DAOQOU010000154         7666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5305_length_7666_cov_6.074892, whole genome shotgun sequence.
ACCESSION   DAOQOU010000154 DAOQOU010000000
VERSION     DAOQOU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_7666_cov_6.074892"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..776
                     /locus_tag="PLM90_11730"
     CDS             24..776
                     /locus_tag="PLM90_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90094.1"
                     /translation="MKSFSAICIGLLICSILNGQVNLKDSCVKANIIQVDLGVGMPAF
                     DMAERFGVHALVGGGYQYKSNHNWLFGVNGNFIYGSIVHEDSLMSNLRNEAGYVIGSD
                     GLGYNPILWESGFTAKWETGKITNIWSINPNSGLTFIGGIGLMQHKIWIYIDETVVPQ
                     LDETNRKGYDRLSNGLMLSQFAGYYLFSNKYFVNFRGGIEVVEAFTQNRRIVNYDTQM
                     TDDEMRFDMLINLKFSWNLPIYNQPKTRYYTN"
     gene            778..2097
                     /locus_tag="PLM90_11735"
     CDS             778..2097
                     /locus_tag="PLM90_11735"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ90095.1"
                     /translation="MKVLVVVFFVGVIAVLKLLFLQPLIYSVIVRIYGFGIRVASIFG
                     GKARAWVKGRENLFERLQQQVQSGGRKTIWMHCASLGEFEQGRPLLETLKKQHPQAYI
                     VLSFYSPSGFEVRKDYAGADVVTYMPLDTAANASRFIDIIKPDVAIFVKYEFWYHHLK
                     ALKSRNIPTYLASGIFRKGQLFFKWYGRLHKDMLRCFTTLFVQEEGSLALLEKIKMTH
                     GVLAFDSRFDRVKEIADKPVSIPLIAQFVEGFNVLVAGSTWPKDERVLQRVFYKSLVF
                     VNFKLILAPHHIEGKWMQRTMKLFKKFSVKYSEAEKLPYEELMAKRIMIIDNMGMLSQ
                     LYRYADVNYIGGGLNKGVHNTLEAAVYGKPLIIGPRYKKFHEAVGLVKANAALVITDA
                     DDLLNRINLMNQFPFVWKGVGIDAAKYVTDRLGGTETMADIISKKIS"
     gene            complement(2098..2994)
                     /locus_tag="PLM90_11740"
     CDS             complement(2098..2994)
                     /locus_tag="PLM90_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90096.1"
                     /translation="MEIALPKFKTTDLPMDAKITRVEVLLDKLNMVFAEVKSDGSLDP
                     LELELLRKYTRQLAETLDLPLEAGHNITPVTTIPPVEKIQEVNLPPAPVEIPVEPIMV
                     KTEEVIMKIVADDEIETPIVIKPKIETVPVVEEKLPVVEKEIIVEPVKPVLEENPVKE
                     IIPPAAEKQEELPPAKKETPVKKPLVKSVESDDDEDEQTAGLSDKLQTGNKKTLADKI
                     SAHKAKDLKSVIPLNDKLLITKSLFGGDKDAYENTIKALNNMQSLTSAEQFIHGELKS
                     KFNWQDEDAIERLIEMVQLKFS"
     gene            3018..3587
                     /locus_tag="PLM90_11745"
     CDS             3018..3587
                     /locus_tag="PLM90_11745"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HMZ90097.1"
                     /translation="MFIEPHLKGEGRGWIEVICGSMFSGKTEELIRRLRRARIANLKV
                     EIFKPTIDTRYDAVNIVSHDDTQIVSTPVANSLNILLMADDTEVVGIDEAQFFDEQIV
                     EVCEKLAQRGTRVIVAGLDMDYRGKPFGPMPQLLAIADYITKVHAICVQCGNIASYSY
                     RKTTDKSQVLLGEKNEYEPRCRSCFLKES"
     gene            3687..5279
                     /locus_tag="PLM90_11750"
     CDS             3687..5279
                     /locus_tag="PLM90_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90098.1"
                     /translation="MKKNLVLITSLFIYTVMQAQAPLNSPNLLLPGTDLIRDAKTVTK
                     ADLHKATLDEGDRALNYYWVNYSDAVDFAYNGWALENLASLPLWPDSTALVVTGDGSD
                     FYWYGHGYAHMFDPISDYISDFVDENYTVIGNASDWWNEDHSFKIDSVRFYYFYDRFN
                     TSYTDTLKVYVMAPNSTCYQEGFYLDNDASGDFDEGIDISLFMSKYNYVNNRPSGTYT
                     EYTFLLGDDDTASFNIASLNIPLALTINKNSPDHNVGIAWQFIPGQPYSTGDTLLDFS
                     DPPLLVTNPLNRFWLLCNEEILESDPASWSEYSNNQDGFASTEVRYNISDGGWNGYYV
                     STYAYVDAFAFEHAYVDWYVAPRGVNFLATDPSPCVSLEKNFTDLSNFAAVPDDATYY
                     WEFGDGAVSFDRNPVHTYPIPGSFEVCLSVDYGGEAFDYCKNVAVDYCTAIEDIENLT
                     SFSLYPNPADDVLNVNLTFGVAQQATIEIVNMAGQVMITENVNATVNYNTSMDVSSLP
                     AGIYMVRINSGIQSSVRTFVVE"
     gene            complement(5809..>7666)
                     /locus_tag="PLM90_11755"
     CDS             complement(5809..>7666)
                     /locus_tag="PLM90_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90099.1"
                     /translation="FFNPNGTSANITWNVCIAVNPADAQRIYIGGQIQSWTWDGASGS
                     WTPMTSSGYPTWYPKYIHADQHYITFPADYNPDTKDIMYFCSDGGIARSLNASSQYPD
                     FGTLNKGLNFYQSHGVASGINGEVMGGAQDNGTHLVDFQQNSEFQGFEWLGGDGGRVE
                     ISKIRPEYLFGTFFYVGASGNGAALRRSVNSGSSASNMYDPNIDDDQDGLADGGTDFV
                     TTFVMWENYKLYETFKDVLVGGTVEYPAGSGNFYVEGDEVNYEGRTITLTRTGLSESR
                     FYHAVKNKLWMTTGALFNSTEAPEWFKVIPVTSGNISAIEYDATGDIVYVGTESGRLY
                     RLDGMLDAAYEYVDTDGDPGTAGVFAPADAGITQFTYANVFPGKITGISIDRENPDQI
                     AISIGGFGVDQNVWTSANALDGDAAVFNCVSDAGSLPNIPVYDILVHAADPDKLLIGT
                     EFGVWSYSISGGGSWTQENGAVGGGVGPGNVPVFEIREDFVRETDCMAIYMGTHGNGY
                     YRTTTLAAGDCDFSTVSAGPIQEEIIAGITIAPNPASYTTTATMTLERNASSVTISLV
                     NLMGATVRNYGTSAYNEGTHNINLDLKGITPGTYLVVFNIDGVVISRRLVVM"
BASE COUNT         2109 a         1632 c         1672 g         2253 t
ORIGIN      
        1 aataactttg cacgcccata tttatgaagt cattctccgc tatttgcata ggcctcctga
       61 tctgttcaat tttgaacggg caggtgaatc tcaaagacag ctgtgttaag gcaaacatta
      121 ttcaggtaga cctgggcgtt ggcatgccgg cattcgacat ggctgaacgt tttggtgtcc
      181 atgcacttgt gggaggcggt tatcagtata aatccaatca caactggctt tttggtgtca
      241 atggcaactt catttatggc agcattgtgc acgaagattc attgatgagt aatttacgca
      301 atgaggcagg gtatgtaatt ggcagcgacg gcctgggata taatcccatt ctttgggaaa
      361 gcggatttac ggcaaaatgg gaaacaggga aaatcaccaa tatctggagt atcaacccga
      421 attccgggct tacgtttatt ggcggaatag gcttgatgca acataaaatt tggatttata
      481 ttgatgaaac tgttgtgccg caattggatg aaaccaatcg caaaggctac gaccgtttgt
      541 cgaatgggtt gatgttgagt cagtttgcag gatattattt attcagcaat aaatatttcg
      601 tcaattttcg tggggggatt gaagttgtgg aggcatttac acaaaaccgc cgcatagtga
      661 attacgatac acaaatgaca gatgacgaaa tgcgatttga tatgttgatc aatttaaaat
      721 tcagttggaa tctgccaatt tacaatcaac cgaaaactag atattatacg aattgacatg
      781 aaagtattag ttgttgtatt ttttgttggg gtcattgccg tattaaaact attgttttta
      841 cagccgctca tatattcggt aatcgtacgc atatatggct tcggcatacg cgtggcgtca
      901 attttcggtg gtaaggcaag ggcatgggtg aagggaagag aaaatctttt tgagcgattg
      961 cagcaacaag tacaatcagg aggaagaaaa acaatctgga tgcactgtgc ctcactcgga
     1021 gaattcgagc aaggacgtcc attacttgaa accttgaaaa agcaacatcc acaagcatat
     1081 atcgtattaa gtttttattc tccttccgga tttgaagtca gaaaagatta tgcgggtgcc
     1141 gatgttgtta cttacatgcc attagatacc gcagcaaatg cttcgcgatt tatagatatc
     1201 atcaaacctg atgttgccat ttttgtgaaa tacgaattct ggtatcatca cttaaaagcg
     1261 ttgaaaagca ggaatattcc aacctatctc gcttcaggta ttttccgcaa aggacaatta
     1321 ttctttaaat ggtatggtag attgcacaaa gacatgttgc gctgcttcac cactttattt
     1381 gtgcaggaag aaggctcact ggcattgttg gaaaaaatta aaatgacaca tggcgtactt
     1441 gccttcgaca gtcgatttga tcgcgtgaaa gagattgccg acaagccggt ttcaattcct
     1501 ttgattgcac aatttgttga aggatttaat gtgttggtgg caggtagcac ctggccgaaa
     1561 gatgagcgtg tacttcaaag agtattttat aaatcactcg tatttgtaaa tttcaagctg
     1621 atattggcgc cacatcatat tgaaggcaag tggatgcagc gcacgatgaa attatttaag
     1681 aagttctcgg taaaatattc agaagccgaa aaattacctt atgaagaatt gatggcaaaa
     1741 cgcattatga tcatcgacaa tatgggcatg ctgtcgcagt tgtatcgtta tgccgatgtt
     1801 aattatatcg gaggtggatt aaataagggc gtccacaata cattggaagc tgctgtatat
     1861 ggtaaaccgc tcattattgg tccgcgatac aaaaaatttc atgaggccgt tggtttagta
     1921 aaagccaatg ccgcattagt gattaccgat gccgatgatc tgctgaaccg catcaatttg
     1981 atgaatcagt ttccatttgt atggaaaggc gttggtatag acgctgcaaa atacgttacc
     2041 gaccgtctag gcggaactga aacgatggct gacattatca gcaagaaaat ttcgtgatca
     2101 gctgaatttc aattgcacca tttcaatcaa tcgctcaatg gcgtcttcat cctgccaatt
     2161 gaatttcgat ttcaattccc cgtgaataaa ctgttcggct gaggtcaagg actgcatatt
     2221 attaagcgcc ttgatcgtat tttcataggc atccttatcg ccaccaaata atgatttcgt
     2281 aattagcaat ttatcattca atgggatcac gcttttcaaa tcctttgctt tatgcgcgct
     2341 gattttatct gcaagtgttt ttttattgcc tgtttgtaat ttatcgctca gtccggcagt
     2401 ctgctcatct tcatcatcat cgctctctac cgatttcacc aatggctttt taacaggtgt
     2461 ttcttttttt gccggaggca gctcttcctg tttttctgct gcaggtggaa taatttcctt
     2521 cacaggattt tcttcaagaa caggttttac cggttcaaca attatttcct tttcaacaac
     2581 aggaagtttt tcttcgacaa caggaactgt ttcaattttt ggtttgatga caatgggcgt
     2641 ttcaatttca tcgtctgcaa ctattttcat gattacctct tctgttttca ccattattgg
     2701 ctcaacagga atttcaacag gtgcgggtgg taagtttact tcttgtatct tttctaccgg
     2761 agggatggta gtaacgggtg tgatattatg accagcttct aagggcaaat caagtgtctc
     2821 ggcaagctga cgggtatatt tcctgagcaa ctccagttcc agggggtcaa gagagccatc
     2881 acttttcact tcggcaaaca ccatattcag tttgtcgagc aacacttcca cacgtgttat
     2941 tttagcatcc ataggtaaat ccgtagtttt gaatttcggt aaggcaattt ccataccatt
     3001 aaaacgagta caaatgtatg tttattgaac cgcatctaaa aggtgaaggg cgtggatgga
     3061 tagaggtcat ctgcggatcg atgttctcgg gtaaaaccga agagctcatt cgcaggttgc
     3121 gccgggcacg tattgccaat ttgaaagtag agattttcaa acccaccatt gatacccgtt
     3181 atgatgcagt gaatattgta tcacatgacg acacacagat cgtttctacg ccggtagcca
     3241 attcattgaa tatattgttg atggcagatg acaccgaagt tgtaggcata gatgaagccc
     3301 aatttttcga tgaacaaatc gttgaggtat gtgaaaagct tgctcagcgc ggcacaagag
     3361 ttattgttgc cggtctcgat atggactacc gtggcaagcc atttggtccc atgccgcaat
     3421 tactggccat agccgattat atcacgaaag tacacgctat ttgtgtgcaa tgtggaaata
     3481 tcgcttccta ttcctatcgc aaaaccactg ataagtcgca ggttctactc ggagaaaaaa
     3541 atgaatacga accgcgctgc agatcctgtt ttcttaagga gagttaagta agcaataaag
     3601 tccgttaact tacattatcc tgacctttgg aaggtggatt tatgtatatt tgtggatatt
     3661 attattagct caactaaacg catgctatga aaaaaaatct tgtactcatt accagtttgt
     3721 tcatctacac ggttatgcag gcacaggctc ctctcaatag tcccaatctg ctgttgccgg
     3781 gaaccgacct catccgcgat gcgaaaacgg ttacaaaagc tgatttgcat aaggctacat
     3841 tagacgaagg cgatcgcgct ttgaattatt attgggtgaa ttactccgac gcagtagatt
     3901 tcgcctataa cggctgggcc cttgaaaacc tggcttcatt gcctttgtgg ccagattcaa
     3961 cagcattggt tgttaccggc gatggtagcg acttctactg gtatggtcac ggatacgcac
     4021 acatgttcga tccgatttct gattatatct cagattttgt tgatgaaaac tataccgtaa
     4081 tcggcaatgc cagcgactgg tggaatgaag atcacagctt caaaatcgac tcagtgcgct
     4141 tctactattt ctacgatcgc tttaacacaa gctatactga tacacttaaa gtgtatgtaa
     4201 tggctccaaa ttctacctgc taccaagaag gtttttacct tgataatgat gccagcggcg
     4261 acttcgacga aggtatcgat atcagcttgt tcatgtcgaa gtataactac gtaaacaacc
     4321 gccctagcgg aacctatacg gaatacacct tcttgttggg tgatgacgat actgcaagtt
     4381 tcaatatcgc cagcttgaat atcccattgg cgctgacaat caacaaaaac agccctgatc
     4441 ataacgtggg tattgcatgg cagttcattc cgggacaacc ttacagcaca ggcgacactt
     4501 tgcttgactt ctctgatcct ccactactgg ttaccaaccc gcttaaccgc ttctggttgc
     4561 tttgtaacga agagatcctt gaaagcgatc ctgcttcctg gtcagaatat tctaacaacc
     4621 aggacggttt tgcctctaca gaagttcgct acaatatctc tgatggcgga tggaatggtt
     4681 actatgtgag cacttatgct tatgttgatg cttttgcttt cgaacatgct tatgttgatt
     4741 ggtatgtggc tccaagaggt gtaaacttcc tggcaacaga tccttcacct tgcgtaagcc
     4801 ttgaaaagaa cttcactgat ctttcaaact tcgcagcggt tcctgatgat gctacttact
     4861 attgggaatt tggtgatggc gctgtaagct tcgatcgcaa ccctgttcac acctacccaa
     4921 ttcctggttc atttgaagtt tgtctctctg tagactacgg cggtgaagcc ttcgattatt
     4981 gcaaaaacgt agctgtggac tattgcactg ctatcgaaga tattgagaat ttgacctcat
     5041 tcagcctgta tccaaaccct gctgatgatg tgttgaatgt taacctcact tttggtgtgg
     5101 ctcaacaagc aactatcgaa atcgtaaata tggcaggtca ggtaatgatt actgaaaatg
     5161 taaatgcaac tgttaattac aacacttcta tggatgtaag cagtcttcct gccggtatct
     5221 atatggtgcg tatcaacagc ggtattcaaa gctctgtgag aactttcgta gtagaataat
     5281 tctccggata tacctctttc aaagagccga ttcattgcga atcggctctt ttttatttgt
     5341 tgaatatcaa tgcattatat aacattatct gcaatcctac agcgtcggtt ttgtaatgct
     5401 ccattgctat tacccctcta ccctgcccgt aaataggctg tttcaggcga atacagctgt
     5461 tatagatggg aagttttatc agcttacagt ttcaaataac caacataact tactgaatat
     5521 taatagatta tcgacttgaa ctgagggatg aaattgcatt ggttgtagtg taccatacca
     5581 gtgaaagacg ccaggcactt taaggtcatc agggcatgta tatacccgtt tcccgactgc
     5641 gtccttaggt gtgattgaag catcatttgt tgcatttata tttattaatg gaaacaggga
     5701 ggaagcactt tttcctgcca ccaatacttc aagcacacac ctcgaaatgt agggcaaaaa
     5761 aaagcccccg ccgtggaggc agaggcttca ataatatctt gctaaggatt acatcaccac
     5821 gaggcggcga gatatcacca cgccatcgat attgaatact accaggtaag ttcccggagt
     5881 gattcctttg aggtcgaggt tgatattatg cgttccttca ttataggcac tagttccata
     5941 gtttctaaca gtagcaccca tgaggttcac aagactaatc gtcactgaag aggcattgcg
     6001 ttccagtgtc atagttgccg ttgtggtata agatgcaggg tttggtgcga tagtaatacc
     6061 tgcgatgatt tcttcctgga taggacctgc gcttacggtt gagaagtcgc aatcaccggc
     6121 agccagcgtt gttgtacgat agtaaccatt tccatgagtt cccatgtata tggccatgca
     6181 atctgtttca cgaacaaagt cttcgcggat ttcgaataca ggcacgttac cgggacctac
     6241 gccaccgcca actgcaccgt tttcctgcgt ccagctgcca ccacctgaaa tagagtagct
     6301 ccatacaccg aattctgttc cgataagaag tttatcagga tctgcggcat gaaccaggat
     6361 atcataaaca ggaatgtttg gcaagcttcc cgcatcagaa acgcaattaa acactgcagc
     6421 atcgccatcg agcgcgtttg ctgatgtcca tacgttttga tcaacaccga aaccaccgat
     6481 agagatagct atctgatcag gattttcacg atcgatgctg atacctgtga ttttgcccgg
     6541 gaatacgttt gcatacgtaa actgcgtgat tccggcatct gcaggagcaa acacgccggc
     6601 tgttccggga tctccatcag tatcaacata ttcgtaggca gcatcaagca taccatcaag
     6661 gcgatacaag cgtccgctct cagttccaac atatactata tcaccggttg catcgtattc
     6721 gatagcgctg atgttaccgc ttgttacagg gataacttta aaccattcag gagcctcagt
     6781 agagttaaag agtgcacctg tagtcatcca cagtttattt ttaacagcat ggtagaaacg
     6841 tgattcagag agacctgtgc gtgtaagcgt aatggtgcga ccttcatagt tcacctcatc
     6901 accttccaca tagaagtttc cgcttcctgc aggatattct actgttccac ctaccaatac
     6961 atctttaaat gtttcgtaga gtttataatt ctcccacatc acgaatgtgg ttacgaagtc
     7021 agttccacca tcggcgagac catcctgatc atcgtcgata ttagggtcat acatattgct
     7081 tgctgagctt ccgctgttca cagatctgcg cagtgcagca ccgtttccag aagcgccaac
     7141 atagaagaag gttccgaaga ggtattcagg acgtattttg cttatttcaa cacgaccacc
     7201 atcgccacct aaccattcga atccttggaa ttcgctgttt tgctggaagt cgacaaggtg
     7261 cgttccgtta tcctgagcac cacccattac ttcgccattg ataccgctgg ccacgccatg
     7321 cgactggtag aagttaagac ctttattgag ggttccgaaa tcagggtact gagacgatgc
     7381 gttcaaagaa cgggcaatac cgccatctga acagaaatac ataatgtctt tagtatccgg
     7441 attgtaatcg gcagggaaag tgatatagtg ctgatcggca tggatatatt taggatacca
     7501 ggtaggataa ccggagcttg tcataggcgt ccagctacct gaagcaccgt cccatgtcca
     7561 cgattgaatt tgaccaccga tataaatacg ttgtgcatcg gcagggttta cggcgataca
     7621 cacgttccag gtgatgtttg cgcttgttcc gttaggatta aagaac
//
LOCUS       DAOQOU010000155         7593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5380_length_7593_cov_5.430618, whole genome shotgun sequence.
ACCESSION   DAOQOU010000155 DAOQOU010000000
VERSION     DAOQOU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5380_length_7593_cov_5.430618"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLM90_11760"
     CDS             complement(<1..463)
                     /locus_tag="PLM90_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90100.1"
                     /translation="MKLHRIICLGLGAIVLSITSLSAQTGSSSSSSSGNQGQSGSSSQ
                     SGYIPNSSSSSASQGGAPGPIRIEPNTPDAITIFQTTNASPGVNTAKATKYDFITVPD
                     DPTRTRIYTLKNGLKVYLSQNKAEPRIQTYIAVRTGSNNDPKETTGLAHYLE"
     gene            complement(492..2273)
                     /locus_tag="PLM90_11765"
     CDS             complement(492..2273)
                     /locus_tag="PLM90_11765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMZ90101.1"
                     /translation="MQSFVVSARKYRPASWHDVIGQDQVVTTLKNAIRTDHLAQSFLF
                     CGPRGVGKTTCARILAKTINCENITKEGEACNECESCKSFNENASFNIHEIDAASNNS
                     VEDIRSLVDQVRYAPASGKFKIYIIDEVHMLSPQAFNAFLKTLEEPPTYVKFILATTE
                     KHKIIPTILSRCQIFDFNRIRIRDMVKQLEIICKSEGITCTEEALHLVAQRADGAMRD
                     ALSIFDRITSFSGKSIEAKDVIDNLNILDYEYFFRITECAVSEDATTALVLFDEIINK
                     GFDGDVFINGLAEHFRNLLMCKEEKTIGLLDTSEAAIEKYTSQSQMIPASFLLSGLNL
                     LNSADIQYRGAKNKRLHVELVIMKLCYIHNAVSALASGSVEKKTPDLNKTVQLKPKIE
                     PQREAEPLPVNPSFRPESKSEVPPVQKVVVQNNAINPPVPEQIETVMIPKKTDLRQSV
                     RKEEEQRKLEVPVVQVTQEKILIEPEDFFVAWKQFAAFIRAEKQAEAIIMDHAAVSID
                     DRNIVTVKLDSSYEVNILKGVHNELHDFIKQSLQNNTFEINFVVEAAKQEKVKRPYTA
                     QEKYKKMEELNPLIRDLKEKLGFELDY"
     gene            2428..3609
                     /locus_tag="PLM90_11770"
     CDS             2428..3609
                     /locus_tag="PLM90_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ90102.1"
                     /translation="MIWLGICLHTQAFYAQSFISNGDFETMTLCPDTTSMLVLAEGWF
                     EANLANPDYFNACDIEGVVGVPENFIGSESAHSGSGYAGIYVAENDGPVYREFLESAL
                     TGSLMPNHAYAVSLYLSVADISSCFPTSLCIYLSSEPMLEPLTDGYLTHYELLQQMLC
                     LEPGNIIDDMDGWYHAYFCFKATGDEHFITIGNNYSEAQAPCINITGGAQKAYVYIDD
                     VSLTEMPVTSVEMDTFICKGSAISIDALDHIEQPANSEFSIVWNDGGTGSKRTFTEEG
                     IYTAEIDNGCSTDTFRITLHWRMDCPEIFYFPNAFSPNNDGLNDRYYPQIENITIAQI
                     DIYDRWGALVYSGIQDPNGWDGTVNGKDAEIGLYMYVIRYTTNISEKSVEKYGALQLI
                     R"
     gene            complement(3620..4963)
                     /locus_tag="PLM90_11775"
     CDS             complement(3620..4963)
                     /locus_tag="PLM90_11775"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90103.1"
                     /translation="MTNVYLYKIVLGGVRKHLVCLSLIVSGCLGAKAQNYISYFTGDT
                     TDVETIPTAGTVLMGGATENDNAMRWFLERAGGGDVVVLRTTGDDGYNDYFYSDLGVI
                     VNSVETIICNDAASGADPYVIHQVENAEALWFAGGDQWDYIQYWRNTPLEDVFNVLIN
                     DKKIPIGGTSAGCAIQGEAYFSAENGTITSTQALHDPYHNLMTLGYSDFIDNPGTKQI
                     ITDTHYDNPDRRGRHIAFLARLYQETGVPYYGIGVEEYTAVCIDENNIAHVFGSYPYY
                     DDYAYFLQVNCVEPNTPETCVDGSLLTWDRSSAAVKVIKMAGMDFGEGSVDLNDWKTA
                     VGADFQWEDWSVDNAIFSALEMTSPINCDTLLTVENNILKNVVIYPNPATDHISLQNT
                     GSDFDYRILNISGDCIFKGTMNSNDQINVRDIPAGLYVIEIRSDNYLDYQSFLKH"
     gene            5011..6189
                     /locus_tag="PLM90_11780"
     CDS             5011..6189
                     /locus_tag="PLM90_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HMZ90104.1"
                     /translation="MEPAPRVDFNDTATAFSGRTDKALKWEYQLFRLMNNPAATNVLT
                     SLASLSIKMHLPVKWAIKKTVYEQFCGGETLEETKLVLSELERFGIGAIIDYGVEGKE
                     SEAEFERTKNELIHIIQFAAGKKNIPFISCKVTGLTAFATLEKLNSRVELTTAEQKEK
                     DALYGRMFAIAGAAADAGIGLFVDAEESWIQDAIDDLTFELMRRFNKQKAVIFSTPQM
                     YRHDRLAFSKRSLEDAVQHGYILGLKPVRGAYMDKERKRAQELGYQDPIQPDKAATDR
                     DYDAVIQFSLDHIDKIALCAATHNENSSLLLANALNDRGIAHNHPHIWFSQLFGMSDQ
                     ITYNLAKSGYNVAKYLPYGPVKDVIPYLIRRAQENKSVSGQMSRELSLIHKEIKRRGI
                     "
     gene            6301..7458
                     /locus_tag="PLM90_11785"
     CDS             6301..7458
                     /locus_tag="PLM90_11785"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HMZ90105.1"
                     /translation="MPFYHKMGQVPQKRHTQYRQPDGSLYHEELFSTEGFSNTYSLLY
                     HLYPPTKIKQVGEAYSVEPKVVLSRQLKHRSLKGFKIAPVDDFLESRKAVLVNSDLHI
                     SLAAPKKSMTDYFYKNVDADEMLFIHEGTGKLHTAYGEIAFEYGDYIIIPRGTLYQIS
                     FDTEQNRLLIVESFSPIEFPKRYLNKVGQLMEHAPFCERDIKTPVNLQTYDQQGDFYF
                     KIKKQGIIYPYHYANHPFDLIGWDGYVYPWAFSIHNFEPITGRVHQPPPVHQTFEARN
                     FVVCSFVPRMYDYHPLAIPAPYNHSNIDSDEILYYVDGDFMSRKSVERGQITLHPGGI
                     PHGPHPGTYEGSIGKKETKELAVMIDPFKPLQLTEEALAIEDGDYYLSWLA"
BASE COUNT         2191 a         1548 c         1605 g         2249 t
ORIGIN      
        1 gttccaggta atgcgcaaga ccggttgttt ccttcggatc attatttgaa cctgtgcgaa
       61 cagcaatata tgtttgaatt ctgggttcag ctttattttg agacagatac accttcaatc
      121 catttttcaa cgtataaata cgtgttctgg taggatcgtc gggaacggta atgaaatcat
      181 acttcgtagc cttagccgtg tttactccgg gcgatgcatt tgtagtctgg aaaatagtta
      241 tggcatcagg tgtgttgggt tcaatgcgaa taggaccggg agcgccgcct tgactggcac
      301 tgcttgatga actgttaggt atatagccgg attgactgct gcttcccgac tgtccctgat
      361 ttccggagga cgagctcgag gaagatcccg tttgagcaga tagacttgtg atagaaagca
      421 ctattgcgcc taatcctaaa caaatgattc gatgtagttt catgttattg atcatctact
      481 ttttatgaaa attaataatc caattcaaat cccaattttt cttttaaatc tcggatgagg
      541 ggatttagct cttccatttt tttatatttt tcttgcgctg tatatggacg cttcactttt
      601 tcctgtttgg cagcctcaac aacgaagttg atttcgaaag tattattttg caatgattgc
      661 ttgataaagt cgtgtaattc gttgtgcaca ccctttaaga tatttacctc ataggatgaa
      721 tctaatttca cggtaactat atttctatcg tcgatagaaa ctgctgcatg gtccataata
      781 atggcttctg cttgcttctc tgcgcggata aatgcggcaa actgtttcca ggcaacaaaa
      841 aaatcttccg gctcaatcag gattttttct tgtgtgactt gcactaccgg cacttccagt
      901 ttgcgctgct cttcctcctt gcggactgat tgtcgaagat ccgttttttt agggatcatg
      961 accgtttcta tttgttccgg aacaggtgga tttatggcat tattctgcac gaccactttt
     1021 tgcaccggcg gcacttctga tttcgattcc ggtcggaaag agggatttac aggaagcggt
     1081 tctgcttctc gttgaggttc aattttaggc tttaactgaa cagttttgtt caggtcagga
     1141 gttttttttt ccacggagcc ggaagcaaga gctgaaactg cgttatgaat ataacatagt
     1201 ttcatgatca ccaactctac atgcaagcgt ttattttttg ctccgcgata ttggatatca
     1261 gcactgttca acaaattcag tccgcttaat aaaaaggaag caggaatcat ttgcgactgc
     1321 gaggtatatt tttcaattgc agcttctgat gtgtcgagta atcctatcgt tttttcttcc
     1381 ttacacatca gcagattgcg aaaatgttcg gcaagtccat taataaacac atcgccgtcg
     1441 aaacctttat tgatgatttc gtcgaatagc accagagcag ttgtggcatc ttccgataca
     1501 gcgcattcgg taatgcggaa gaaatattcg taatcgagaa tatttaaatt atcaataaca
     1561 tctttcgctt caattgactt gccggaaaaa gatgtgattc tatcgaagat acttaatgca
     1621 tcgcgcatag cgccatcggc acgctgggca acgagatgca aagcttcttc ggtacaggta
     1681 atcccttcac ttttacagat gatctccaat tgcttcacca tatcgcggat gcgaatacga
     1741 ttgaagtcaa agatttgaca tctgctgagg atggtaggaa tgatcttgtg cttttcagtg
     1801 gtagcgagaa taaattttac ataggtagga ggctcctcta atgtcttcaa aaaagcattg
     1861 aatgcctgag ggctgagcat atgcacctcg tcgataatgt atatcttaaa tttcccgctt
     1921 gcaggggcat atcgcacctg atcgaccaga cttcgaatat cttctacgga gttgttagaa
     1981 gccgcatcga tttcatgaat attgaaagat gcattttcat taaaagattt acaactttcg
     2041 cattcgttgc aggcttcacc ttctttggta atattttcgc aattgatagt tttcgccagg
     2101 atacgggcac aggtggtttt acccacacca cgcggaccac agaacaggaa cgactgggca
     2161 aggtgatcgg tgcgaatggc atttttcagg gtagtaacta cctgatcctg tccaatcaca
     2221 tcatgccatg acgccggcct gtattttctg gctgaaacaa caaaactctg cacggggtaa
     2281 agatatacaa tttagcgtgt cgtatccctg attttaccat cagattacac aagtaccatg
     2341 cccaaaacct aaatttgtaa tgaaatctat taaccctaat tgcccaacgt aaccagctat
     2401 attgttaaac tttcccggat tggtttgatg atctggcttg gcatttgttt gcacacacaa
     2461 gcattttatg cacaaagttt tatcagcaat ggcgatttcg agaccatgac cctttgccct
     2521 gatactacgt ctatgctggt attggctgag ggatggtttg aggccaatct ggctaatcca
     2581 gattatttca atgcctgcga cattgagggg gtagttggtg ttcctgaaaa tttcattggc
     2641 agtgaatcag cgcattcagg aagcggatat gcaggcattt atgtagctga aaatgatggc
     2701 cctgtgtatc gcgaattttt agaatctgca ttaacaggtt cgctgatgcc caatcacgcc
     2761 tatgcggtaa gtttatattt aagtgttgcc gatatcagtt catgtttccc gacatcattg
     2821 tgtatctatt tgtcttcaga accaatgctg gaaccattaa ctgatggata tcttacacat
     2881 tacgagctat tgcagcaaat gttatgtctt gaacccggaa atatcattga tgacatggat
     2941 ggctggtatc atgcctattt ttgtttcaag gctacaggcg acgaacattt tattaccatt
     3001 ggaaataact attccgaagc acaagctcca tgtataaata ttaccggagg ggcacagaaa
     3061 gcctatgtat atattgacga tgtttcactg actgaaatgc cggttacgag cgttgaaatg
     3121 gataccttta tttgcaaagg atctgcaata agcattgatg cactagacca tattgaacaa
     3181 cctgccaata gtgagttttc aattgtttgg aacgatggtg gcacaggcag taaacgcaca
     3241 tttactgagg aaggcatata tacggccgag attgacaacg ggtgttcaac agatactttt
     3301 cgtattacat tacattggcg gatggactgt ccggagatat tttatttccc aaatgcattt
     3361 tctccaaata atgatggatt aaatgatcgc tattatccgc aaatcgagaa cattactatt
     3421 gcccaaatag atatttacga cagatgggga gcattggtat attcaggcat tcaggatcca
     3481 aacggatggg atggaaccgt aaacggaaag gatgcagaaa ttggtcttta tatgtatgta
     3541 atcagataca ccaccaatat ctcagaaaaa tcggtagaaa aatacggtgc tttacaatta
     3601 ataagatgaa tcccggaatt tagtgcttca gaaaagattg ataatctaaa taattatcac
     3661 ttcgtatttc aattacataa agtcccgcag gtatgtctct aacattaatc tgatcatttg
     3721 aattcatagt tcctttaaat atgcaatctc cggaaatatt aagaatacga taatcaaaat
     3781 cgctgccggt attttgtaag gatatatggt ctgtagcagg attcggatat attaccacat
     3841 ttttcaggat attattttcc accgtcaaca acgtatcgca atttatcggc gatgtcattt
     3901 ccaatgcact gaatatcgca ttgtcaaccg accagtcctc ccactgaaaa tccgctccca
     3961 ctgcagtttt ccagtcgttc agatcaacac ttccttcccc aaaatccatt cccgccattt
     4021 ttataacttt cacagcagca ctacttctgt cccatgtgag caaagaacca tctacacaag
     4081 tttccggtgt attcggttcc acacaattta cttgcaagaa atatgcataa tcatcataat
     4141 agggataact gccaaacaca tgtgcaatat tgttttcatc aatacaaaca gcggtatatt
     4201 cttcaacgcc gataccataa tatggcacac cagtttcttg atataatcgc gcaagaaaag
     4261 cgatatgacg accgcgtctg tccgggttgt cgtaatgtgt atccgttata atttgttttg
     4321 tgccagggtt gtcgataaaa tcgctatatc ctaatgtcat taaattatgg tatggatcgt
     4381 gtaatgcttg tgttgatgta atggtcccat tttctgcgga aaaatatgct tctccttgaa
     4441 tggcacaacc ggcacttgtt ccgccgattg ggattttttt atcgttgata aggacattaa
     4501 atacatcttc caatggtgta ttcctccaat attgaatata atcccattga tcaccgcctg
     4561 cgaaccataa tgcttctgca ttttctactt gatggattac atatggatcg gcgcctgaag
     4621 ctgcatcgtt gcatataata gtttcaacag agttaacaat aaccccaaga tctgaataga
     4681 aataatcatt atagccatca tcaccggtag tgcgcaacac aaccacatcg ccaccaccgg
     4741 cacgttcgag gaaccatcgc attgcattat cgttttctgt tgctccaccc atcagcactg
     4801 tacctgcagt tggtattgtt tctacatcag tagtatcacc ggtaaaatag gaaatatagt
     4861 tttgcgcttt cgctccaagg caaccggaaa cgatgagact aagacatacg aggtgttttc
     4921 ttacaccccc taagacgatt ttatataaat aaacattagt catcccataa aaatatacaa
     4981 ccacgtatat ttgcacaaat tcatcatatt ttggaacccg caccgcgtgt tgactttaac
     5041 gatacggcta ctgcattttc aggccggaca gacaaggcat taaaatggga atatcagttg
     5101 ttcaggctca tgaacaatcc tgctgctacg aatgtactca cctccctggc atcattatcg
     5161 ataaaaatgc atttgccggt aaaatgggcc atcaaaaaga ccgtttatga gcaattttgc
     5221 ggaggagaga ccttagaaga aaccaagctc gtgctcagcg aattagagcg ttttggcata
     5281 ggagcaatca ttgattatgg tgttgaagga aaggagtctg aagcggaatt tgagcgcacc
     5341 aagaatgagc ttatccatat catccaattt gcagcaggca agaaaaacat tccattcatc
     5401 agttgcaagg ttaccggtct gactgcattt gctacccttg agaagttgaa ttcgagggtc
     5461 gagttgacaa ctgcagagca gaaggaaaaa gatgcccttt acggacgcat gtttgccatt
     5521 gccggtgctg ctgccgatgc cggtattgga ctattcgtag atgcggaaga aagctggatt
     5581 caagatgcca tagatgacct gacctttgaa ttgatgcgtc gatttaacaa gcaaaaagcc
     5641 gttattttca gcactccgca gatgtatcgc cacgacagac ttgcatttag caagcggtca
     5701 ttggaagacg ccgttcaaca tggttatatt ctgggtttga aacctgttcg aggcgcgtat
     5761 atggacaagg agcgcaaaag ggctcaggaa ctgggttatc aggatcctat tcaacccgat
     5821 aaggctgcca ctgaccgcga ttacgacgcc gttattcaat tttcgctcga tcatattgat
     5881 aaaatcgcct tatgtgctgc cacacacaat gaaaacagca gtttgctatt ggcaaatgcc
     5941 ctaaacgacc gtggaatagc gcacaatcat ccacatattt ggttttcgca gttgtttggt
     6001 atgagcgacc agatcactta caacctggca aaatcaggct acaatgtagc gaaatacctg
     6061 ccttatggtc ctgtcaagga tgtaatccca tatttgatta gaagagccca ggaaaacaaa
     6121 tccgtaagcg gtcaaatgag cagggaactt tccctgattc ataaggaaat caaacgcaga
     6181 ggtatttaag tctaatatac tttctcagat aattgtcaga acccaccgta ggttgacaaa
     6241 agtcaccgcg cgcgggatga ttattttgta attttacgga gcatacttaa caggaatacc
     6301 atgccatttt accataaaat gggacaggtg cctcaaaagc gccataccca gtatcgacag
     6361 cctgacggaa gcttatatca tgaggagttg ttttcaaccg aaggcttcag caatacttat
     6421 tcattactgt atcatctata tcctccaacc aagataaagc aggtaggtga ggcatacagt
     6481 gtcgagccaa aagtggtgct gagtcgtcaa ttaaagcatc gctctttgaa agggtttaaa
     6541 attgcacctg ttgacgattt tttagagagc agaaaggccg tgttagtaaa tagcgattta
     6601 catatttcac tcgccgctcc taaaaagagt atgactgatt atttttacaa gaatgtagat
     6661 gccgatgaaa tgcttttcat acatgaggga accggcaaat tacatactgc atatggcgaa
     6721 attgcatttg agtatggcga ttacataatt atccctcgcg gaacactcta tcagataagt
     6781 ttcgatactg agcaaaacag attattaata gttgaaagtt tttcgcctat cgaatttccg
     6841 aagcggtacc ttaacaaagt tggtcaatta atggagcatg cgccattttg tgagcgcgac
     6901 atcaagacac ctgtcaattt gcaaacgtat gatcaacagg gcgattttta ttttaaaata
     6961 aaaaagcagg gaattattta tccctatcat tatgccaacc atccttttga tttgatcgga
     7021 tgggatggat atgtgtatcc ttgggccttt tctattcaca attttgaacc aataacagga
     7081 cgcgtacatc agccacctcc ggttcatcaa acttttgaag caagaaattt tgttgtttgc
     7141 agttttgttc cgcgcatgta tgattatcat ccgctggcaa ttcctgctcc atataatcac
     7201 agcaatatcg atagcgacga aatattgtat tatgtcgatg gagattttat gagtcgcaag
     7261 agtgtggagc gcggacaaat cacgttacat ccaggaggta taccgcatgg tccgcatccc
     7321 ggtacttatg aaggcagcat aggcaaaaaa gaaactaaag aattagccgt gatgattgat
     7381 ccattcaaac cattgcaatt gactgaagaa gcgttggcaa ttgaggatgg agattactat
     7441 ctatcatggc tggcatagtg tggaagtgtg caagtgtgca agtgtgcaag tgtgaatgtg
     7501 tggaagtgtg caagtgtgaa tgtgtgggaa tgtgggaatg tgaaagtgtg aaagtgataa
     7561 ggtgagtagg aggataaata tatgatatgc tga
//
LOCUS       DAOQOU010000156         7588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5388_length_7588_cov_5.899376, whole genome shotgun sequence.
ACCESSION   DAOQOU010000156 DAOQOU010000000
VERSION     DAOQOU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5388_length_7588_cov_5.899376"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..1618)
                     /locus_tag="PLM90_11790"
     CDS             complement(392..1618)
                     /locus_tag="PLM90_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ90106.1"
                     /translation="MTLRENISLAFRAIKGNRLRSVITITIIALGLTALIGILTAIDA
                     LESSINSNFAMLGANSFSIRNFNELSNGNEKPNPPISYKEATSFKDRYTYSGADVSIS
                     AIFIQGSAEVKQAAKKTNPNVDVYGVDENFIPVVGYTIKEGRGFSNIELNSATRVVVL
                     GSDVAEKLFGERDNPIDKGVTINDQYFRVIGILEPKGSSFMQSDNRVLIPLTAARSIF
                     PQVGRDSYILSVGVKTAVDMEPAIGEATGLMRQVRRLRLGEDDDFSIAKSDSVVSIML
                     DSLSYVALGALFIGFITLVGAAIGLMNIMLVQVNERTREIGVSMAIGANRKVIRRQFL
                     IESIVICILGGIFGIILGIAIGNLLAVALHANFILPWLWIILGLTVCVITGLAAGYIP
                     ARRASRLDPIEALRYE"
     gene            complement(1705..3615)
                     /locus_tag="PLM90_11795"
     CDS             complement(1705..3615)
                     /locus_tag="PLM90_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90107.1"
                     /translation="MKTLTLSAYSAASAQLLGLSKDTSAQMIYQDLDPDFGIGPDFPV
                     DLNDDGISEMRIHGHGWWTFTSDMVYSSRQIEFFNYAEIATSAGVLQMFEGDTVDADL
                     DFGTADQFFFYGYNDDGWIDIDEGASWVNNNSYIGMRFDVSGEIHYGWLRVELHQDDI
                     HDMPTLYVKELAWNATADAPAPINAHVGVALFPELSDVGETNTATDLQLHFQKADDES
                     HISSYRVILYPGFPAPTLAEANALSADRYTELIPVGADITMNFTETTRDIDGNLLLPG
                     TYYSAIILSVADGIAVTENDLSSHSNNMQFIYLEAPVPLDVYLQSTGYDDDITGLYGR
                     FYLTDTAVASVRAYLSDSYFEADELLLLDPEYYIETIPDAVGTHYMWFTADKHIYTSD
                     APILFEDYVLSIVTVPNGITNSIPELGTDVAHFTYPNYDVMPTVTLVDSTGTGADIQL
                     HYPMFINEDDLELYRIFIAKADVDIEPAYAYTVPPSKRLDISPAGIDLDVNLSSITLD
                     TDGNSIKYNQAYRVYVALKGDFYPDYYSLATPSAEFILEEPVIESIFSQNNKAAPNIW
                     VNDHLIHIAGATNEMHTLTLLNASGQEVYRTTFNGTEVAVEAPELPAGVYVVGVESEH
                     GVHHQTIFLQGK"
     gene            complement(3690..5624)
                     /locus_tag="PLM90_11800"
     CDS             complement(3690..5624)
                     /locus_tag="PLM90_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90108.1"
                     /translation="MNRYTITAYSATAGIFLLHSNVTDAQMVHVNVDPDAHVGPYFDY
                     NLDLNADGINEVFFNCGEFWTGSSFGGEFHGYSSQWIYLYPTGTFATSIGQIKHLVVG
                     DTVDADMLFNDVDPLFFYGQRDIIAGGGWVDIVEGSEWVNNEGFFGLKFPIDGETHYG
                     WIRVELIQLDPDYLPQMYVKEFAYNDTPDAPAPITIYNADIAKSLVLSDVGETNTPED
                     LQLTFNKADNEITVSEYRVALFKGAIPPTVAQIDSLTPDRYISVFPTGDDLSVSFSAD
                     ILDINGDTIIPGYLYRAIVLSVADGTYAIESDLSVPSNYTYFSLDVAPATYEVELLTD
                     SIATDISGFIGSFGMMDTSVSAVRLYITANEMNVEELLALDEPYYMETIPVVGINTVH
                     FTSDKLIYEAGDPALFNLYYLHVVSIPDSITNSIPALSVQSGRFLYNQYPVAPEITII
                     GYAGNSSDILLEFPQLPDEASLEVYRVFVCKAGTVVNSELVAGMLPIHYESVYPSGVD
                     LHMDLTACNLDSDGDEIIEGQPYVAYVAMRCTCDDYFYSITIPSSEFILGEPEVGIDN
                     MATDELIITDNVILIPNGLESSTLQISNASGQMMFEYMIQSGDRDIKLPPFPAGIYIA
                     YIKNEQHIYAKKFVITSSGG"
     gene            5834..>7588
                     /locus_tag="PLM90_11805"
     CDS             5834..>7588
                     /locus_tag="PLM90_11805"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HMZ90109.1"
                     /translation="MAFDLDMIRQVYAAYPARIEAARKLLNRPLTLTEKVLYAHLSEG
                     MASKAYERGKDYVDFGPDRVAMQDATAQMALLQFMSAGKKKVAVPSTVHCDHLIQAKT
                     GADIDLATALDSNKEVYDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGVGGADAVDVMAGFPWELKMPKLIGVKLTGKMNGWTSAKDVILK
                     VAGILTVKGGTGAIVEYFGDGAAAISCTGKGTICNMGAEIGATTSIFGYDDSMRRYLQ
                     STDRQDVVDLADAVAEHLNGDAEVYANPAAYFDQLIEINLDELEPHVNGPFTPDLAWP
                     LSKLKDAVITNGWPATLEVGLIGSCTNSSYEDLTRAASLARQAAEKKLKAKAEFTITP
                     GSELVRYTVERDGLLEDFEKIGGVVLANACGPCIGQWARHSVDPNRKNTIITSFNRNF
                     AKRNDGNPNTHAFVASPEIVTALAIAGDITFNPITDTLVNEEGVAVKLDEPTGLELPA
                     KGFAVEDAGYIEPAADGSKVEVLVDPASKRLQLLAPFTAWEGTDLKGLKLLIKVKGKC
                     TTDHISMAGPWLKFRGHLD"
BASE COUNT         2294 a         1680 c         1531 g         2083 t
ORIGIN      
        1 cttcacaacg caccttgagc aaaatgataa aatcacaatg ccaaatgacc caataattgt
       61 atgaaatcgt gaagcgtgac tcgtgagtcg tgaagcgtga atcgtgattc gtgattcgtg
      121 agtgtggtgc gtactttagg atcgggtata cgatgcgcat atgcatttac tcaccactca
      181 ccatttttca atcgcggagt ttgcggagtt tgcggagttt gcggaggaat cacattcacg
      241 agtctcaact caccattcac cactcaccac tcaccactca ccactcacca ctcaccattc
      301 accactcacc actcaccttt tttcaatcgc ggagtttgcg gaggaatcac attcacgagt
      361 ctcaactcac cattcaccac tcaccactca cctactcata cctcagcgcc tcaataggat
      421 ccaacctgga agctctgcgg gccgggatat atcctgctgc aagtccggtg attacacaaa
      481 ctgtcaatcc caaaataatc cataaccatg gtaatatgaa gtttgcatgt agtgcaacag
      541 ccagaagatt gccaattgca atgccaagaa taattccgaa tataccgccg aggatacaaa
      601 ttacaatact ctcaataaga aactggcggc gaatgacttt tctatttgca ccaatggcca
      661 tgctcacacc aatttctctg gtgcgttcgt ttacttgtac gagcatgata ttcattaatc
      721 cgatagcagc accaaccaat gtgataaatc caataaataa tgcacccagt gccacataag
      781 aaagactatc aagcataata ctcaccacac tatcactttt cgcaatgcta aaatcatcat
      841 cctcacccaa tcgcaaacgg cgcacttgac gcatcagacc ggttgcttca ccgatggcgg
      901 gttccatgtc gacagcagtt ttaacaccga cagataaaat gtaagaatct ctacccacct
      961 gagggaaaat acttcttgca gctgtaagcg gaatcaacac gcgattatcg ctttgcataa
     1021 agctgcttcc ttttggttcc agaattccga tcactctgaa atactgatcg ttgatggtaa
     1081 cacctttatc gataggatta tctctttcgc caaacaattt ttcagcaaca tcactaccca
     1141 gcaccacaac acgtgtggcg gaatttaatt cgatattgct gaaacccctt ccctctttaa
     1201 tagtatatcc gacaaccgga ataaaattct catcaacacc atagacatca acattcgggt
     1261 ttgttttttt agctgcttgt tttacttctg cagacccttg aatgaaaatg gccgaaatag
     1321 atacatcggc acccgaataa gtatagcgat ctttaaatga agtagcttcc ttataactga
     1381 tgggtggatt tggtttctca tttccattgc tcagttcatt gaagttgcgg atagaaaatg
     1441 aattagcacc caacatggca aaattcgagt tgatagaact ctctagtgca tcaattgccg
     1501 ttagaatacc aatcagggca gttaatccga gggcaataat agtgatggtg atcacacttc
     1561 gcaggcggtt tcctttgatg gctctgaagg ctaatgatat gttttccctt aatgtcacgc
     1621 cgataccctt tccacaaagg tagaacctgc atgtgttaaa atgttaaaat gccatggaaa
     1681 tttcagcaac aacctcaatt ccactcactt tccctgaaga aagatagttt ggtgatgcac
     1741 accatgctcg ctttccaccc caactacgta aactcctgcg ggtagctcag gtgcttctac
     1801 tgcaacctca gtaccgttga aagttgttct gtagacctct tgtccggatg cgttaagcaa
     1861 ggtcaatgta tgcatttcat ttgtcgcgcc tgcaatatgt attaaatggt cattcaccca
     1921 aatattcgga gctgctttat tattttggga aaaaattgat tcaataacag gttcctccaa
     1981 aataaattct gctgaagggg tagcaagtga ataataatca ggatagaagt cgcccttcaa
     2041 ggcaacatat acccgatatg cttggttata tttaatacta ttgccatcag tatcaagggt
     2101 aatggaggac aaattcacgt ccaggtcgat gcctgcaggg gaaatatcta atcgtttaga
     2161 gggcgggact gtatatgcat aagcaggttc aatatctacg tctgccttcg ctatgaaaat
     2221 gcgatacaat tccaagtcat cttcgttgat gaacatagga taatgtaatt gaatatctgc
     2281 gccggtaccg gttgaatcaa caagtgtgac tgtaggcatg acatcgtaat taggatatgt
     2341 aaaatgggct acatcggtgc cgagttcagg tattgaatta gtgatgccat ttggcactgt
     2401 aactatactg agcacataat cctcaaataa gataggcgca tcggaggtgt agatatgttt
     2461 atcagctgtg aaccacatgt aatgagtacc tactgcatca ggtattgttt caatataata
     2521 ctcagggtca agcagcaaca attcatcagc ctcgaaataa ctatcagata aataagctct
     2581 cactgaagcc acagcggtgt cggtcaaata aaacctgcca tataatccgg taatatcatc
     2641 gtcgtaacct gttgattgca aatatacatc cagaggcaca ggtgcttcca aataaataaa
     2701 ttgcatgtta tttgagtgcg aagagaggtc attttcagtg actgcaatgc catcagcaac
     2761 tgataagatt atcgcgctat aataagttcc gggcagcaat aaattgccat caatatctct
     2821 ggttgtttct gtaaaattca tggtgatatc tgcaccaaca ggaatcaact ccgtatatct
     2881 gtcggcactt agtgcatttg cttctgcaag tgttggagca ggaaatccgg gatacaagat
     2941 caccctataa ctactgatgt gactttcatc atctgccttt tgaaaatgga gttgcagatc
     3001 ggtagcagta tttgtttcac ctacatcaga taattccgga aatagcgcta caccaacatg
     3061 ggcattgata ggtgccgggg catccgccgt tgcgttccat gcaagttcct ttacataaag
     3121 tgttggcata tcatgaatat catcctgatg caattcaaca cgcaaccaac cataatgaat
     3181 ctcgccactc acatcaaaac gcataccgat ataactatta ttattgaccc aggatgcacc
     3241 ctcatcaata tctatccaac cgtcgtcatt ataaccgtaa aagaaaaatt gatctgctgt
     3301 tccaaaatcc agatcagcat caaccgtatc accctcaaac atctgaagca caccggcaga
     3361 tgtggcaatc tcagcataat taaaaaactc gatctgtcga gaggaataca ccatatcact
     3421 ggtgaacgtc caccaaccgt gtccatgaat acgcatttcc gaaataccat catcattcaa
     3481 gtcgaccgga aaatccggac caataccaaa gtcaggatcc aggtcctgat agatcatttg
     3541 cgcacttgta tctttggaaa gaccaagtaa ctgcgctgat gctgcagaat aggcggagag
     3601 ggtaagtgtt ttcatgtagg atgatttatt tttcaccttg gcgagtgttc actattaaag
     3661 ttttgtgttg caaggtacgt aacacattat tatccaccgg aggacgtaat cacaaatttc
     3721 ttggcataaa tatgctgttc attcttgata taggcgatgt atatacccgc cgggaatgga
     3781 ggaagtttta tatctctatc accggattga atcatatatt cgaacatcat ttgtccagat
     3841 gcattagaaa tttgcaatgt tgaactttcc aaaccatttg ggatcaggat gacgttgtca
     3901 gttatgatta attcatctgt tgccatatta tcaatcccta cttccggttc acccaaaata
     3961 aattctgatg atgggatggt aatactataa aaataatcat cgcaagtgca gcgcatggct
     4021 acataggcaa catatggctg accttcaatt atttcatctc cgtcggaatc cagattacaa
     4081 gctgtcagat ccatatgcag gtcgactccc gatggatata cagattcata atgaatgggg
     4141 agcataccgg caacaagttc tgagttcaca acggttccgg ctttacaaac aaaaacacga
     4201 tacacttcta aactagcctc gtcggggagt tgtggaaatt ctagcaggat atcactcgag
     4261 ttacctgcgt aaccaattat ggtgatctca ggagcaacag gatattgatt atagagaaat
     4321 cttccgcttt gaacagagag cgccggaatt gaatttgtaa tactatcagg tattgagacc
     4381 acatgtaaat agtacaaatt aaataatgca gggtctcctg cctcataaat taatttatcc
     4441 gatgtaaaat gcacagtatt aattccaaca acaggaattg tttccatata atatggttcg
     4501 tcgagtgcca aaagttcttc aacattcatt tcattggccg tgatatataa ccgaacagca
     4561 gaaacactgg tatccatcat tccaaatgat cctataaacc cggaaatatc ggtagcaata
     4621 ctatcggtaa gcagttcaac ttcatatgtt gcaggtgcta catccaatga aaaataagta
     4681 taattagatg gcacagatag atcactttca atggcataag taccatctgc aactgataat
     4741 acaattgccc tgtataaata accaggaatt atggtatcgc catttatgtc caatatgtcg
     4801 gcactaaatg ataccgataa atcatctcct gtcggaaata cagaaatata tctgtcgggc
     4861 gttaaactgt ctatttgagc aactgttgga ggaattgctc ctttaaaaag tgccactctg
     4921 tattcgctga cggttatttc attgtcggct ttattaaaag taagttgcag atcttcaggt
     4981 gtattagttt ctccgacatc gctcagcact aatgatttgg caatatctgc attgtaaata
     5041 gttattggag ccggcgcatc cggtgtatca ttgtaggcaa attccttaac atacatttgc
     5101 ggtaaataat cgggatctaa ttgaattaat tccacccgaa tccatccata atgtgtttca
     5161 ccgtcaattg gaaatttcaa tccgaaaaac ccttcattat taacccattc agatccttca
     5221 acaatgtcta cccatcctcc gcctgctata atatctcttt gtccataaaa gaaaagcgga
     5281 tcaacatcat taaataacat gtctgcatca acggtatctc ctaccacaag gtgcttaatt
     5341 tgtccgatag atgttgcaaa tgtccctgta ggatataaat aaatccattg agatgaataa
     5401 ccatgaaatt cccctccaaa agaggagcct gtccagaatt cgccacaatt aaaaaacacc
     5461 tcattaatcc catccgcatt caaatccaga ttatagtcaa aataaggtcc aacgtgagca
     5521 tccggatcca cattcacatg caccatttgt gcgtctgtaa cattcgaatg gagcaggaat
     5581 atgccggcag tagcagaata ggccgtgata gtatagcgat tcatgaggtg gggtttatat
     5641 caaagataat gtgttttcct cctaaatggt tgcatgaagg tttgtgaaat ttttaaactc
     5701 gtctaaaaaa aaatttatcc tcgtttaata aacgaaaatc ggcaatgatt gttacctttg
     5761 caggcgaaag gcaaaaaatg ccctgtttta agggttttac gcctccaaaa ccgataacaa
     5821 aaatcatcaa gatatggcat ttgacttaga catgatcagg caagtatatg cagcttatcc
     5881 tgcacgtatt gaagccgcac gaaaactcct taaccgacca cttacgctca ccgaaaaggt
     5941 attgtacgct cacctcagcg aaggcatggc gtccaaagct tatgagcgcg gaaaagatta
     6001 tgtcgacttt ggaccggatc gcgttgccat gcaggatgct actgcacaga tggcattact
     6061 gcaatttatg agtgccggca agaaaaaagt agccgttcca tctacggtgc attgcgatca
     6121 cctcattcag gcaaaaacag gtgccgatat cgacctcgct acggctcttg attctaataa
     6181 agaagtctat gattttttag cttccgtatc taataaatat ggtattggat tctggaaacc
     6241 gggcgctggt atcattcatc aggtggtgct tgagaactat gcatttccgg gtggtatgat
     6301 gattggaacc gacagtcaca ccgttaacgc cggcggtctc ggaatggtgg ccattggtgt
     6361 tggaggtgct gatgctgttg acgtgatggc aggattccct tgggagctca aaatgcctaa
     6421 actgatcggt gtgaaactga caggtaaaat gaacggatgg acttctgcaa aagatgtaat
     6481 tcttaaagtg gcaggaatcc tgaccgttaa aggcggaacc ggagcaattg tggaatattt
     6541 tggcgatggt gccgctgcca tttcttgtac aggtaaaggt accatttgta atatgggtgc
     6601 tgaaatcggc gctactactt ccatttttgg ttatgacgac agcatgcgtc gctatttgca
     6661 atctaccgat cgccaggatg ttgttgatct tgctgatgct gttgccgaac acctcaatgg
     6721 agatgcagaa gtgtatgcaa atcctgcagc ttatttcgat caattgattg aaatcaacct
     6781 cgatgaattg gagccgcatg taaacggtcc ttttactccc gatctggcat ggcctttatc
     6841 taaacttaaa gatgccgtga tcacaaacgg atggcctgca actcttgaag tagggctcat
     6901 cggcagctgc acaaactcaa gctatgaaga tctcacacgt gctgcatcat tggcacgtca
     6961 ggcagcagag aaaaaattaa aagctaaagc ggaattcacc atcacccccg gcagtgaatt
     7021 agtgcgttat acagttgaac gcgatggtct gctcgaagac tttgaaaaaa ttggcggcgt
     7081 tgttctggca aacgcctgcg gtccatgcat tggtcagtgg gcgcgtcaca gtgttgatcc
     7141 aaacagaaaa aataccatca tcacttcttt caaccgcaac tttgcaaaac gcaatgatgg
     7201 taatccgaat acacatgcat ttgtagcctc accggaaata gttacagctc ttgctattgc
     7261 aggcgatatt accttcaacc caattaccga tacactggtt aacgaagaag gtgttgccgt
     7321 aaaactcgat gagccaacag gtctggaatt acctgcaaaa ggatttgcgg tagaagatgc
     7381 gggatatatt gaaccggcag ctgatggcag caaagtagaa gtacttgttg atcctgccag
     7441 caaacgtttg caattattgg cccctttcac ggcgtgggaa ggcacagatc tgaaaggatt
     7501 aaaattgttg attaaagtaa aaggaaaatg caccaccgac catatctcaa tggcaggtcc
     7561 atggctgaaa ttccgcggac atctcgat
//
LOCUS       DAOQOU010000157         7517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5472_length_7517_cov_6.093273, whole genome shotgun sequence.
ACCESSION   DAOQOU010000157 DAOQOU010000000
VERSION     DAOQOU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_7517_cov_6.093273"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..918)
                     /locus_tag="PLM90_11810"
     CDS             complement(379..918)
                     /locus_tag="PLM90_11810"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMZ90110.1"
                     /translation="METIQTERLLLTKYTPERYHEMFTTMTEGELKKHLGETGNHCYA
                     VMRKRWEQGLRTHNRSFTFFQIRERATDRIIGGGGFHNWATDMFRAELGYALENEFDK
                     RKGYMTEALSVIVPYGFNVMQLHRMEAFIGPNNIPSQKLVEKWGFKKEGHLREHFLRG
                     EVYEDSLVFGLLRGEWGNG"
     gene            1065..4364
                     /gene="ileS"
                     /locus_tag="PLM90_11815"
     CDS             1065..4364
                     /gene="ileS"
                     /locus_tag="PLM90_11815"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HMZ90111.1"
                     /translation="MSTKKYPESKSLNLPLIEKEVLQYWKSDNTFQKSVSSRPENKPF
                     VFYEGPPSANGTPGIHHVLARSLKDLVCRYKTMQGFRVERKSGWDTHGLPVELGVEKT
                     LGITKEEIGKTISVAEYNEHCRKEVMKYTAQWEELTNIMGYWVDMEDPYVTYDHNYIE
                     TLWNLLKRLYDKGLLYKGFTIQPYSPAAGTGLSSHELNQPGTYKPVKDTTATAMFRIL
                     PEHIPAHLMTQHAEVFFLAWTTTPWTLPSNTALAVGANITYVQIDTFNPYTQKPISVI
                     LAKDRVPAYFKDEQKVHALISEEPAGWKPVSEFTGKQLEGIRYEQLWPYHQPTNGDAF
                     RVITGDFVTTEDGTGIVHIAPSFGSDDFRVAKQNGIGSLTLVDKEGKFVDGMGDLSGR
                     FVKNYKDEEKWENPDVIIAIKLKESNRAFRVEKYEHNYPHCWRTDKPVIYYPLDSWFI
                     RTTAAKERLIALNKTINWKPASTGEGRFGQWLENLVDWNLSRSRYWGTPLPIWRSEDG
                     AEELCIGAFEQLQKEVDRARDLNPHLPADWNAASNFDPHRPFVDEIVLRSASGKPMRR
                     ESDLIDVWFDSGAMPFAQWHWPFENTEVFDRKFPADFIAEGVDQTRGWFFTLHVIATL
                     INDSVAYKNVISNGLVLDKNGNKMSKRLGNAVDPFHTINNYGADATRWYMITNAQPWD
                     NLKFDTDGIVEVQRKFFGTLYNTYAFFALYANVDEFRFEGNEIPVSNRSELDRWVISL
                     LNSLTAQVTEALNDYEPTRAGRAIQDFVGDHLSNWYVRLSRRRFWKSDDSADKQAAYQ
                     TLYTCLDTISRLMAPIAPFMSEWLFRNLNGVTGKHQAESVHLSTFPVADPTLIDRDLE
                     DSMQLAQDISSTVLSLRKKQNIKVRQPLSKIMIPVLSDTTRRHIERVAELVKSEVNIK
                     EIEFISEDSGVITKKIKPNFKALGPKLGGDMKSAAGLISGFSQQQISELERNKEIILD
                     LGNKSFTLSSEDVEILSEDIPGWLVAPAGTLTVALDITVTDALLFEGHARELVSQLQR
                     MRKDMNLAITDRISVTYHTGAELSQAIEDYKQYICAEILADSLETSDGLSDTGKISVN
                     DIEISIQIKKV"
     gene            4369..5499
                     /locus_tag="PLM90_11820"
     CDS             4369..5499
                     /locus_tag="PLM90_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HMZ90112.1"
                     /translation="MAKKIVKATKSSASKSAPTKTKTAAKKAAPAPKKVVKPVAKKAA
                     PKAAKKTTAKPVAKKAAPKAPKKAVAKPVAKKTAAKPQPKAKAPAKKAVAVKTPLKPV
                     VKKEVPKAPVKKEAPKAPVKKEVAKKPEAKKPEVKKPEVKKPEVKKVEVKKPEAAKKV
                     APVEKTPIKKAEKQPEVKKPEVKKPEPVKVVAPEVQPVATKKTAAKKSTKKNAPPPPP
                     APTYDNPEIKLGPVKILPPKRELNPQPVKVTSTVLDNRTRYSDEELQEFKELITGKLE
                     AARNELKYLQEQISRSSEMGDDSDARFKGLEDGTSTSEREYLSQMASRQITYIGHLEK
                     ALIRIENKTYGICRETGKLISKERLRAVPHATLSIEAKQGKG"
     gene            5504..6175
                     /locus_tag="PLM90_11825"
     CDS             5504..6175
                     /locus_tag="PLM90_11825"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HMZ90113.1"
                     /translation="MKKRSLVAFAVIIGVLLIDQLLKFYIKTNFTLGGGFDILGLSWA
                     KIHFVENEGMAFGLELGGTYGKLLLTLFRLLAVGGIGYYLYTLIRDKANFGLIISIAL
                     VCAGAMGNIFDSVFYGIYFSESTFTQVAEFMPAGGGYTTVLHGHVVDMLYFPIADGHW
                     PEWMPFIGGKYYHFFRPVFNIADSAITTGVLSILIFQRRIFASPEMESAKEKPTIAEE
                     TAREE"
     gene            6243..7193
                     /locus_tag="PLM90_11830"
     CDS             6243..7193
                     /locus_tag="PLM90_11830"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HMZ90114.1"
                     /translation="MHDAAAFEAFYRQEIVPDLDRLEKIRKEQLKRVTFMWSSAIISL
                     VLAFLLTMPVLVVIFLFIALGVYFYLFGFNRKRVDFKTEYKRVVIGKLATYISDQLVF
                     TPNIFIPESTYNQSKIFLSDPDIYTGEDLVTGKIGNTQIQFCELHTKDRQTDSRGRTT
                     YVTIFKGIFFIADFNKHFNGQTYVLSDFGERFLGAFGRLMQSINVGRPDVVRLEDPEF
                     EKEFVVYSSDPVEARYILSPAMMQRLLEYRKKTGSKIQCSFLNNNIYLAMPMKENLFE
                     PSLRKSVMTMADVEGFYKDLLFCTSIVDELDLNTRIWTKE"
BASE COUNT         2217 a         1724 c         1698 g         1878 t
ORIGIN      
        1 gtgggaatgt gagaatgtgg gaatgccagc ctcgccgcag gcgggtggga atgtgagaat
       61 gtgggaatgc cagcctcgcc gcaggcgggt gggaatgtgg gaatgccagc ctcgccgcag
      121 gcgggtggga atcccgctgg ttgggatgtt acggagctct atgtgggaat caatattcac
      181 atcgcatacc agatacttaa gtacgcacca cactcacgtg tcacgaatca cgatattagt
      241 atctagaacc aagctcgcct cgcctccggc gggtatctag tatctagaca aatgcagtgg
      301 aatgtgcttt gaattttctc tggtatcaga tcataaaaaa ctcaccattc acgactcacc
      361 actcacccac tgacccactc acccattccc ccattccccc ctcaacaatc caaataccaa
      421 agaatcctca tacacttcgc cgcgtaaaaa atgttcgcgc aaatgacctt cttttttgaa
      481 gccccacttc tctaccaatt tctgtgaagg aatattattg ggtccgataa atgcttccat
      541 tctatgaagt tgcataacat taaatccgta cggcacaatc acagataatg cttcggtcat
      601 atatcctttc cgtttatcaa attcattttc taatgcataa cccagctctg cacgaaacat
      661 atctgtcgcc cagttatgaa atcccccacc cccaattatt cggtccgttg cacgctcgcg
      721 aatttgaaaa aaagtgaagc tcctgttgtg cgtgcgcaat ccctgctccc atcgtttacg
      781 catcaccgca taacaatgat tgcctgtttc tcccaaatgc tttttcaatt ctccctctgt
      841 catggtggtg aacatttcat gatatcgctc aggtgtatat tttgtaagta gtaagcgctc
      901 cgtttgtatc gtttccatcc aacgaaaata aacgatagcg cgattttctg taaacacttc
      961 cttccttcgg gcctgcttcc gcacatgaaa aaccctacgc tagcctcaat cttcgtaact
     1021 ttgcgccctt taagcaccgg tcgtaaaaga gctgaatagt aatcatgtcg acgaaaaagt
     1081 atcctgaatc aaaatcacta aacctcccat taatcgaaaa ggaggtctta caatattgga
     1141 agagcgataa cacctttcaa aaaagcgtgt catcgcgccc cgaaaacaaa ccgtttgtat
     1201 tttatgaagg cccgccctca gcaaatggca ccccgggtat tcaccatgta ctcgcccgtt
     1261 cactgaaaga ccttgtttgt cgctataaaa ccatgcaagg cttccgtgta gaacgcaaaa
     1321 gcggctggga tacacacgga ttacctgttg agttgggagt agaaaaaaca ctgggcatca
     1381 ccaaagagga gatcggcaaa accatcagcg tggccgaata caatgaacat tgccgcaaag
     1441 aggtgatgaa atacaccgct caatgggaag agctcaccaa tatcatgggt tactgggtag
     1501 atatggaaga cccgtatgta acctacgacc ataactatat cgaaacactg tggaatctgc
     1561 tgaaacgact gtacgacaaa ggtttgctgt acaaaggatt caccattcag ccttattctc
     1621 ctgctgcggg aacgggattg agctcacacg aactcaacca accgggcacc tataaaccgg
     1681 tgaaggatac cacggcaact gccatgttcc gcatcctgcc cgaacatatt ccggcacatt
     1741 tgatgacgca gcacgcagaa gtgttcttcc tggcctggac caccacacca tggaccctcc
     1801 ctagcaatac agctttagcg gttggagcaa atattaccta cgtccagatt gacaccttca
     1861 acccatatac acaaaaacct attagcgtaa ttctggctaa agaccgcgtt cctgcttatt
     1921 ttaaagatga gcaaaaagta catgcattaa tttctgagga acctgcggga tggaaaccgg
     1981 tgtcggaatt cacaggaaag caacttgaag gaattcgtta tgagcaacta tggccttatc
     2041 atcagccaac aaacggagat gcatttcgtg tgatcaccgg cgattttgta accacagaag
     2101 acggtaccgg cattgtgcat attgcgccaa gtttcggttc tgacgacttc cgcgtagcaa
     2161 agcagaatgg tataggctca ttaacattgg tggacaagga gggaaaattt gtcgacggca
     2221 tgggcgatct gagcggtcgc tttgtgaaga attataagga cgaagagaaa tgggaaaacc
     2281 ccgatgttat tattgcgata aagctaaaag aaagcaatcg cgccttccgc gttgagaagt
     2341 atgaacacaa ctatccgcat tgctggagga cagataaacc cgtaatatac tatccgcttg
     2401 attcgtggtt tatccgcact actgccgcga aagagcgatt gatagccctc aataaaacca
     2461 tcaactggaa gcctgcaagc acaggcgaag gtcgttttgg tcaatggctg gaaaacctgg
     2521 tagactggaa cctcagtcgc tcacgctatt ggggaactcc gcttcctatc tggcgcagcg
     2581 aggatggtgc tgaagagctt tgcataggcg ccttcgaaca attacagaaa gaagtagacc
     2641 gcgcccgcga cctcaatcct catcttcccg ccgactggaa tgcggcaagc aattttgacc
     2701 cgcatcgtcc gtttgtagat gaaattgtgt tgcgcagtgc ttcaggaaaa ccgatgcgcc
     2761 gcgaaagcga cctgatagat gtttggtttg attcgggtgc gatgcctttt gcgcaatggc
     2821 actggccttt tgagaacaca gaagtttttg acagaaaatt tcccgccgac tttatcgcag
     2881 agggcgttga tcaaacgcgc ggatggtttt tcacattgca tgtaattgca acgcttatca
     2941 atgattctgt tgcatataaa aacgttatca gcaatggctt agtgctagac aaaaacggca
     3001 acaaaatgtc gaaacgcctg ggcaatgccg ttgatccttt ccatactatt aataattatg
     3061 gtgccgatgc cacccgctgg tatatgatca ccaatgcaca gccttgggat aatctgaaat
     3121 ttgataccga tggcatcgta gaagtgcagc gcaagttctt cggcacactc tataacacct
     3181 acgcattctt cgcattgtat gccaatgttg atgaattccg atttgaagga aatgaaattc
     3241 cggtatcaaa tcgcagtgag ctggatcgat gggttatcag cctgcttaac tcactaactg
     3301 cacaagttac cgaggccctc aacgactacg aaccaacacg tgcaggccgc gccatacaag
     3361 actttgtggg cgatcacctc agcaattggt atgtgcgttt gagtcgccga cgtttctgga
     3421 aaagcgatga cagcgccgac aagcaagcgg cttaccagac tctatacacc tgtctggata
     3481 cgattagccg gttgatggca cctattgctc catttatgtc ggagtggctg ttccgcaacc
     3541 ttaacggcgt taccggcaaa catcaggctg aatccgttca cctgtctaca ttccctgttg
     3601 ccgatccaac tttgatagac agggatctgg aagatagcat gcaactggcg caagacatct
     3661 ccagcactgt attgtcgctt cgcaaaaagc aaaacatcaa ggtgcgtcag cctttgagca
     3721 agatcatgat cccggtgttg agtgacacca cccgtcgaca tattgagcgc gtagccgagt
     3781 tggtgaaaag cgaggtgaac atcaaggaga tagaatttat cagcgaagac agcggtgtca
     3841 tcaccaaaaa gataaaacct aatttcaagg cgctgggccc gaagttgggt ggcgacatga
     3901 aatccgctgc gggcttgatt tccggctttt cacagcagca gatttccgag ctggaaagaa
     3961 acaaggaaat catactggat cttggcaata aatcgtttac tttatcgtca gaagatgtag
     4021 agattctgag tgaggacatc ccggggtggt tagtagctcc tgctggaaca cttacggtag
     4081 ccctggatat cactgtcacc gatgctttgt tgtttgaagg acacgccaga gagctggtaa
     4141 gtcagttgca acgcatgcgc aaggatatga atctggcaat tacagatcgt atttccgtta
     4201 cttatcatac aggggcggaa ctttcgcaag ctatcgaaga ttataagcag tatatttgcg
     4261 ccgaaatttt agcggattcc ctggaaacat ccgacggcct gtcagatacc ggtaagatct
     4321 ccgttaatga tattgaaata agtatacaga tcaaaaaagt atagtgccat ggcgaagaaa
     4381 attgtgaaag ccactaagtc atcggcgtcg aaaagcgccc caaccaaaac gaagactgct
     4441 gccaaaaagg ctgctcctgc ccctaagaag gtagtgaaac ctgttgccaa gaaagctgct
     4501 ccaaaggccg ccaaaaagac aacagctaag cctgtagcta aaaaagcagc cccaaaagca
     4561 cctaaaaaag ccgtggcaaa accggtagct aaaaaaacag ctgccaaacc tcaaccaaag
     4621 gcgaaagcac ctgcaaagaa agctgttgcc gtaaaaactc cccttaaacc ggtagttaaa
     4681 aaagaagttc ctaaagcacc cgtgaagaaa gaagcaccaa aagcgccagt gaagaaagaa
     4741 gttgcaaaaa aaccggaagc caagaagccg gaagtaaaaa agcctgaggt taagaaacct
     4801 gaggtaaaaa aagtggaagt gaagaagccg gaagctgcga aaaaggtagc tcctgttgaa
     4861 aaaacaccaa taaaaaaggc ggaaaaacag cctgaagtga aaaaaccgga ggtaaagaag
     4921 cctgaaccgg tgaaagttgt tgctcctgaa gtgcagcctg ttgccactaa aaaaactgcg
     4981 gcaaaaaaat ctacaaagaa aaatgcaccg ccgccaccac ctgcgccaac atacgataat
     5041 cctgaaatca aactcggacc ggtaaaaatt ctccctccaa aacgagaatt aaatccacag
     5101 cccgtaaaag ttacttccac cgtactggat aatcgcacac gttatagcga cgaagaattg
     5161 caggagttca aagaactgat caccggtaaa cttgaagctg cgcgtaatga attaaaatac
     5221 ttgcaggagc aaatcagtcg ttcaagtgag atgggcgacg attctgatgc acgttttaaa
     5281 ggtctggagg atggcacctc tacttctgaa cgagaatatc tcagtcagat ggcatctcgt
     5341 cagatcacct atatcggtca cctcgaaaaa gcgcttatcc gtattgaaaa taaaacctac
     5401 ggcatctgtc gcgaaacagg aaaactgatt tcaaaagaac gtcttcgcgc agttccgcat
     5461 gctacgctga gcatagaagc taaacaagga aaaggctgat agcgtgaaaa aaagaagtct
     5521 cgttgccttc gctgtaatta ttggtgtgct gctgatcgat cagttgctga aattttatat
     5581 caaaaccaat tttacactcg gcggcggatt tgatattctg ggactttcat gggcgaaaat
     5641 acattttgtc gagaatgaag gtatggcttt cggacttgaa ctcggaggca cttatggcaa
     5701 actgctgctt acgttattcc gcttacttgc tgtaggagga attggttatt atttatatac
     5761 gcttattcgc gataaagcaa attttggtct catcatcagc attgcgctgg tttgtgccgg
     5821 ggcgatggga aatattttcg acagcgtttt ttacggcatc tatttctctg aaagcacctt
     5881 tacacaggtt gcagaattta tgcctgcagg aggtggttat acaacggtat tgcatggaca
     5941 tgtagtggac atgctttatt ttccaattgc cgatggacac tggccggaat ggatgccatt
     6001 tattggagga aaatattatc acttcttccg tcccgttttc aatattgccg atagcgccat
     6061 caccaccggc gtactctcta tcctcatttt tcagcgtcgc attttcgctt ctccggaaat
     6121 ggaatccgca aaggaaaaac ccacaatcgc tgaagaaacc gcccgcgaag aataagtcac
     6181 gctccatccc atctctgttt aagctgttga tttccctata agggtttcac tatctttgtg
     6241 ccatgcatga cgcagccgca tttgaagcat tctaccgcca ggaaatagta cctgatctcg
     6301 accgacttga aaaaataaga aaggagcaat taaagcgcgt gacctttatg tggtcatctg
     6361 ctatcatttc gttggtgctc gcatttttat tgactatgcc ggtgctggta gtcatattcc
     6421 tttttattgc cttaggtgta tatttttatt tattcggatt caatcgtaaa cgtgtagatt
     6481 ttaaaacaga atataagcgt gtagtaattg gtaaactcgc aacatatatt tctgatcaac
     6541 ttgtttttac acccaatatt tttattcctg aaagcactta caatcaaagt aaaatattct
     6601 taagcgatcc ggatatttat accggtgaag atctggtaac gggaaaaatc ggcaatacac
     6661 aaattcaatt ttgtgaactg catacaaaag atcgtcaaac cgatagtcgc ggacgcacaa
     6721 cttatgttac cattttcaaa ggcatatttt tcatagccga cttcaacaaa catttcaacg
     6781 gacaaaccta tgtgttaagc gattttggtg aacgctttct tggtgctttc ggtcgcctga
     6841 tgcaaagtat aaatgttgga cgtccggatg tagtgcgact tgaggatccg gaatttgaaa
     6901 aagaattcgt cgtttattct tcagaccctg ttgaagcgcg ctatattctt tcgccggcaa
     6961 tgatgcaacg cctgctcgaa tacagaaaga aaaccggttc caaaattcaa tgttccttcc
     7021 taaataataa tatttacctc gctatgccaa tgaaggaaaa tttattcgaa ccttcacttc
     7081 gcaaatcggt aatgaccatg gctgatgtgg aaggatttta taaagacctc cttttctgca
     7141 catcaattgt tgatgaactc gatctgaata cgagaatctg gacgaaggaa tgaggggtgg
     7201 aaatgaggaa aaaggagaga ggaaaaagga aagaggggtg agtgggtgag tcgtgagtcg
     7261 tggctcgtga gtgttgttcg tattcgagga tctagtatgc gatgtgaaca tgcatttacc
     7321 tgatacttga tacctgatac ctgataccaa gtctagctac tagatacccg ccggaggcga
     7381 ggcgagcttg gttctagata ctaatatcgt gactcgtgac tcgtgactcg tgagtgttgt
     7441 gcatacgtga ggatcgggta tgcgatgtga atatacattt acctgatact tgatacctga
     7501 tacttgatac ctgatac
//
LOCUS       DAOQOU010000158         7474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5516_length_7474_cov_5.683650, whole genome shotgun sequence.
ACCESSION   DAOQOU010000158 DAOQOU010000000
VERSION     DAOQOU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5516_length_7474_cov_5.683650"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1740)
                     /locus_tag="PLM90_11835"
     CDS             complement(106..1740)
                     /locus_tag="PLM90_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90115.1"
                     /translation="MKRISILATVIAISMSASAQSTTWEAVYNVIQTNCSGCHVAGHE
                     SGLDLSTDATTVYSSLVDIAPTNVTSAGKGYKRVFAGDPYRSFLFSKINNSLALDVNL
                     GAGEGTACPQGAAPLNNKDIELIRQWILYGAQESDTLVDIDLVNAFYDLDGEQSMPSP
                     PAPPAAGTGFQIHFGPFFLWPDTENEYFTKYPVNIPTDVEINRVDTYMGPYSHHFITY
                     KFVEPGVEASIEPGLHEGPDFFGVDLVTAHQYADSIILPPGTAFHWDYGTVLDLNSHY
                     INYSSDKPLACEAYINVYTQPVGTAIQTMYSVLLSNQSFNIPPTGDPYSDEATAFENG
                     HGEDELFIWGISTHTHKLGTDFDIYKRNDDHSRGEHIFDASCGATEGIPGCLDEIYDY
                     QHPPIRYWESMLPIKWKNGIIYEATWVNNGPDYIDFGPLSTDEMMVIFYFYVDDTTGL
                     NLPVAIPEITPQLTGIQLYPNPAHDNIFIQAETDLSKAMISICDLTGKTIQYSEPISM
                     GQDFYQVKLADVAKGIYLLQVITPDGAMFSQKIMKE"
     gene            complement(1808..2755)
                     /locus_tag="PLM90_11840"
     CDS             complement(1808..2755)
                     /locus_tag="PLM90_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HMZ90116.1"
                     /translation="MKIIIAGGTGAIGTAMSTFFLEKGNDVIVLSRQTLPSQRKLKII
                     KWDGEHIGEWRNECEGADVIINLCGKSVNCRYTEKNKLLILQSRLRSTRATGKLIGML
                     KRPPAVWFNAASATIYKGTLEGPQDEYTGEIGSDFSMNVCTDWEREVMLAHTPFTRKI
                     YLRTSIVLDKNVGALPLYKRIASIGLGGAMGSGEQMMSWIHIDDVCRAVLFLIDKKEI
                     EGPVNITSPFPITNAVFMESLRRVLHMPFGVSNPEWMLRIGTSIIGTEMELLMKSRWV
                     YPQKLLAAGFHFNYPEINEALTDFFDTKAASSQVATSGR"
     gene            complement(2752..3549)
                     /locus_tag="PLM90_11845"
     CDS             complement(2752..3549)
                     /locus_tag="PLM90_11845"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMZ90117.1"
                     /translation="MPTPLLTDPRLRKVYWKDLMYLTRSEIAWEVTLSLPWLIGSLIL
                     AARGWYAPALVCSFFFFLTGLRQSHNAQHRSVGISARATEWMLFALSVTMLASMHAVK
                     FNHLRHHKDPLGPSDIEAMSARMPWWMAILVGPFNILKQHGNALKLARPQLRRWVIIE
                     IVVILTGAATALWLIDIPWLSYHVIVMLTGECFTAFFAVWTVHHHCADDKVFSRTVRG
                     KWRTYIFYNMFYHTEHHLFPQVPTCHLHELAKRVDEHIPEISKKTVL"
     gene            complement(3603..4109)
                     /locus_tag="PLM90_11850"
     CDS             complement(3603..4109)
                     /locus_tag="PLM90_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMZ90118.1"
                     /translation="MQLTEAKEKFVQSWGSLGSQWGINRTMAQIHALLLVSHEALSTE
                     DIMETLQISRGNANMNIRELINWQLVQKELRTGERKEFFVAEKDMWKVFRLILRERKK
                     REIDPMIQVIKSLQDIEGSSDQPDVREFREVMEELETLTHKGEKLMDLMIRSDEHWFI
                     NTFLKLIR"
     gene            complement(4222..4623)
                     /locus_tag="PLM90_11855"
     CDS             complement(4222..4623)
                     /locus_tag="PLM90_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90119.1"
                     /translation="MNNEQAIGASRKHIAELHPEVIDWINYSLFVKDEVKSLNHRLME
                     IAHRYTDHDVMAQVEHFQNQFIRQDEASDELLHDLRAAEHVFSEISKTNPAASHVLFD
                     DHVELRDKATTHKTIFEDLRHEFQLFLAKYM"
     gene            complement(4760..5191)
                     /locus_tag="PLM90_11860"
     CDS             complement(4760..5191)
                     /locus_tag="PLM90_11860"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HMZ90120.1"
                     /translation="MSIHLQHLESELEENTRRRGYDFYCEGRVRSVFNINGIEWMAVV
                     QGYDDHVVELRITNDTVMHYACNCNDEEDPCKHVAAVLYYIRKESHKQKRNDPSGYDE
                     MKEEISYLPPHVLRYLILKYAAQSKDFRTFLGEYFNGDTRK"
     gene            complement(5232..7241)
                     /locus_tag="PLM90_11865"
     CDS             complement(5232..7241)
                     /locus_tag="PLM90_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HMZ90121.1"
                     /translation="MDSAQSNFQRVLQFDRRHYSNDQLIQIYKRLLKPRMIEEKMLNL
                     LRQGQVSKWFSGIGQEAVAVAVPYVLEYDEYILPMHRNLGVFTGRDIPFAKLFSQWQG
                     KKNGFSKGRERSFHFGTNEYHIVGMISHLGPQLAVADGIALAAKLKNEKKVTAVFTGE
                     GATSEGDFHESLNVAAVWDLPVIFIVENNGYGLSTPTNEQFRCAQIADKAIGYGMRSM
                     IVDGNNILDMVAALEECRAYCINEQKPVLLECMTFRMRGHEEASGIKYVPKELIEMWG
                     KKDPVQNYEQYLLESAIINEEMIKTFRDEIKSEIEAGVQTVFNEPEVIPDTEEEERDI
                     YAPFTQEVIAPKSNIASQKKFINAISDALKLAMDKYPELIIMGQDIAEYGGAFKVTEG
                     FVQQYGKSRVRNTPICESAILGAGCGLSVKKMKSVIEMQFADFVTSGFNPIINNMAKM
                     YYRWGQNVDSVVRMPTGAGVGAGPFHSQSNEAWFFHTPGLKVVYPSSVYDAKGLLLAA
                     IEDPNPVMFFEHKALYRAISDNIPDDYYTVEIGKAALAQSGNELSIITYGMGVHWAKK
                     ACAQLQIDADILDLRTLLPLDYDAIATTVRKTGKVLVLHEDTLIGGIGGEIAAYISEQ
                     LFNVLDAPVMRCASLDTPVPFSLELEKNFLPESRLIEKLKALASY"
BASE COUNT         2015 a         1661 c         1534 g         2264 t
ORIGIN      
        1 agatgccaag gtgtttaata ttagggtgcg gaatgcatat gtttcgcacc ctaatactat
       61 acatatttgt atgtattact tgagcctttt tgcttataaa aattcttact ccttcataat
      121 tttctgcgaa aacatggcgc catcgggtgt gatgacttgg agcaaataaa tgcctttcgc
      181 tacatcagct aattttactt gataaaaatc ttgtcccatg gaaatcggtt cactatattg
      241 tatggtttta ccggtcagat cacaaatgct gatcatagcc ttcgacagat cagtttctgc
      301 ctggataaaa atattatcat gtgccgggtt tggatataat tgaataccgg tcaattgtgg
      361 tgttatttcc ggtatagcaa ccggtaaatt caaacctgtt gtgtcatcca cataaaaata
      421 aaatatcacc atcatttcat cagtgctcaa tgggccgaag tcgatataat caggtccgtt
      481 gtttacccat gttgcttcat aaatgatacc atttttccat ttaataggca gcatgctttc
      541 ccaatatctg atgggtggat gctggtaatc ataaatttca tcaagacaac ccggaatgcc
      601 ttctgtagca ccacaagatg catcgaaaat atgttcaccg cgcgaatgat catcgttgcg
      661 tttataaatg tcgaaatcgg tacccagttt atgtgtatgt gtgcttattc cccagataaa
      721 caattcatct tcaccgtgtc cgttttcaaa agcagtagct tcatcagaat atggatcacc
      781 ggttggcgga atattgaaac tttgattact caacaaaaca gaatacatgg tttgtattgc
      841 tgtacctacc ggctgcgtgt aaacatttat atatgcttca catgcaaggg gcttgtctga
      901 tgaataatta atataatgtg aattcaaatc taataccgta ccgtaatccc agtgaaatgc
      961 tgttcccgga ggcaaaataa tgctatcggc atactgatgt gccgtaacca aatcaacacc
     1021 aaaaaaatca ggtccttcat gcaaacccgg ttctatagaa gcttcaactc ccggttcaac
     1081 aaatttgtag gtgatgaagt gatgtgaata tgggcccata tatgtatcca ctctgtttat
     1141 ctctacatcg gtaggaatat ttaccggata ttttgtgaaa tattcatttt ctgtatcagg
     1201 ccataaaaag aatggaccga agtgaatttg aaatcctgta cctgctgccg gaggtgcggg
     1261 tggtgatggc atactctgtt caccatctaa atcgtaaaaa gcattaacaa gatcgatgtc
     1321 taccagtgta tcactttcct gcgcaccgta taaaatccat tgacgaatta attcaatgtc
     1381 tttgttattt aatggcgccg ctccttgcgg acaagcagtt ccttcgcctg ctcccagatt
     1441 tacatcaagg gcaagactgt tattgatttt gctgaataaa aaactgcgat atgggtcgcc
     1501 tgcaaatacg cgcttgtaac cctttccagc cgacgttaca tttgtaggag caatgtctac
     1561 gaggctgcta taaacagtgg tcgcgtccgt acttagatcc aatccgcttt catgacctgc
     1621 cacgtgacat ccgctgcagt ttgtttgaat gacgttgtaa acagcttccc aggtggtaga
     1681 ttgggcagat gccgacatgc tgatggcgat aacagttgcg aggatactta ttcttttcat
     1741 aatagccttt gaggctacga agttagcgaa ttctgcaatt gaaaatcgta aattccttgt
     1801 caaatcctta tctgcccgac gtggctacct gcgatgaagc tgctttggtg tcaaaaaaat
     1861 ctgttaaggc ctcattaatt tccgggtaat taaaatgaaa accggcagcc aacaattttt
     1921 gcggatacac ccatctgctt ttcatcagaa gttccatttc ggttccaata atcgatgtgc
     1981 cgatgcgcaa catccattcc ggattcgaaa caccaaatgg catatgcaaa acacgtcgca
     2041 atgattccat aaatacagca ttcgttattg gaaatggcga agtgatattt accggtcctt
     2101 ctatttcttt tttgtcgatg agaaataata cggcgcgaca aacatcatca atatgaatcc
     2161 aactcatcat ttgttctcca ctacccattg ctccgcctaa tccaattgaa gcaatgcgtt
     2221 tgtataatgg caatgcacca acatttttat ctaaaactat tgaagtgcgc agataaattt
     2281 ttcgtgtaaa tggtgtgtgt gccaacatga cttcacgttc ccagtcggtg catacattca
     2341 tactgaaatc actacctatt tctccggtat attcgtcttg cggaccttcc aatgttcctt
     2401 tgtaaatggt tgccgaagcg gcgttgaacc atacggcagg agggcgtttc agcatgccta
     2461 tcaattttcc tgtcgctcgt gtactgcgca ggcgcgattg caaaatgagt aacttatttt
     2521 tctctgtgta tctacaattt acactcttcc cgcagagatt aataatcaca tctgcgcctt
     2581 cacattcatt gcgccattcg ccgatatgct cgccatccca tttgattatt tttaatttgc
     2641 gttgtgatgg caatgtttgt cgcgataata caattacgtc gtttcctttt tcaagaaaaa
     2701 atgttgacat ggcggttcct attgcaccgg ttccgccggc gatgataatt ttcatagtac
     2761 agtttttttg ctgatttcag gaatatgttc gtctacacgt tttgctaatt catgcaaatg
     2821 acatgtaggc acttgtggaa ataaatgatg ttctgtgtga tagaacatat tgtagaaaat
     2881 gtaggtgcgc catttaccac gcactgtgcg actaaaaact ttgtcatcgg cacagtgatg
     2941 atgcacagtc cacactgcaa aaaacgcagt aaaacattca cccgtcagca tcacaatcac
     3001 gtggtagctc agccatggaa tatcgatcag ccacaaggca gttgcagcac cggtcaggat
     3061 cactacgatt tcaattatga cccatctccg caattgtggt cttgccagtt ttagggcatt
     3121 gccatgttgt ttgaggatgt tgaatggacc aacaagaatt gccatccacc agggcatacg
     3181 ggcactcatc gcctcgatat cgctcggtcc gaggggatct ttgtgatgtc tcaggtgatt
     3241 gaatttcacc gcgtgcatcg acgccagcat ggttacactc agggcaaata acatccattc
     3301 ggtagccctg gccgaaatgc ctacggagcg atgttgggca ttgtgcgact ggcgcaatcc
     3361 cgtaaggaaa aagaaaaacg agcaaaccaa agccggtgcg taccatcccc tagcagctaa
     3421 tatcaacgag ccaatcagcc agggtagcga tagtgtcact tcccaggcta tttcactgcg
     3481 tgtgaggtac atcaggtctt tccaataaac ttttctaagt cgggggtcgg taagtagtgg
     3541 ggttggcatg acatttattt ttatattttc agtaatattt gaaagtttat agtcaaaaaa
     3601 aatcagcgta tcagttttag gaaagtattg ataaaccaat gctcgtcgct gcggatcatc
     3661 aggtccatga gcttttcgcc tttgtgtgtg agtgtttcca attcttccat cacctccctg
     3721 aattcacgca catcaggctg gtcgcttgaa ccttctatgt cttggagcga tttgatcact
     3781 tgaatcatgg ggtctatctc gcgttttttg cgttcgcgga ggatgagtcg gaataccttc
     3841 cacatatcct tttcagccac aaaaaactct tttcgctccc ctgttcttaa ttccttttgc
     3901 accaattgcc agttgatgag ctcgcggata ttcatgttgg cattacccct gctgatttgc
     3961 agggtttcca tgatatcttc ggtggacaat gcttcatgac tcacaagcag caaggcgtga
     4021 atctgagcca tagtgcggtt tataccccat tggctgccca gacttcccca gctttgaaca
     4081 aatttttcct tggcttcggt gagttgcata agccaaaggt ataacaactt ttcaaacttt
     4141 caaacatttg tgaaagtttt tttgttaaaa ttaaaaagcc cggctgctga cttccgggcc
     4201 taaggttgga taatcggacc tttacatgta tttggccagg aacagttgga attcgtggcg
     4261 gaggtcttca aaaatggttt tgtgggtagt agccttatcg cgcagctcca cgtgatcgtc
     4321 gaacagcaca tgtgatgcgg cgggatttgt tttcgaaatt tcactgaaca cgtgttctgc
     4381 agccctcagg tcgtgcaaca attcatcgct ggcctcgtcc tgacgtataa actggttttg
     4441 aaagtgttct acctgtgcca tcacatcatg atcggtataa cgatgcgcta tttccatcaa
     4501 tcgatggttg agtgatttta cttcgtcttt tacaaaaagg gaatagttaa tccaatctat
     4561 tacttccgga tgcagctcag caatatgttt gcgtgatgct ccgattgctt gttcattgtt
     4621 catggctttt agattttata cgtcaaatat cgacgcaaat tatcgtgcgg agtatggata
     4681 ttgtcactaa gaatggcgac ttatatcacc tctagcttta ttcaaaaagc atgaacaaat
     4741 aataatgatc aggtgcgact tattttctgg tatcgccgtt aaagtattcg cccaaaaagg
     4801 tcctgaaatc tttgctttgg gctgcgtatt ttaaaatgag ataacgcaac acatgtggag
     4861 ggagataact tatttcctcc ttcatttcat catagcccga aggatcgttg cgtttttgtt
     4921 tgtgcgattc ttttcgaata taatataaca ccgcagcaac atgtttacat ggatcctctt
     4981 catcattaca attgcaggca taatgcatca ccgtatcatt ggtgatgcgc agctccacaa
     5041 catgatcgtc atacccctgc actaccgcca tccactctat gccattgata ttaaatacag
     5101 aacgcactct cccttcgcaa taaaaatcgt atccacgccg gcgggtgttt tcctccagtt
     5161 ctgattcgag atgttgcagg tgaatggaca tgtactattt taatttcagg tcgatatgga
     5221 atcctttcaa attaataaga cgccaaggct tttaattttt caatgagacg cgattctggc
     5281 aagaaattct tttccagttc gagactaaat ggcaccggcg tatcgaggct ggcgcaacgc
     5341 attaccggtg catccagtac attaaataat tgttcgctga tataggctgc aatttctccg
     5401 cctataccac caatcaaggt atcctcatgc aacactaaaa cttttccggt ttttcttacg
     5461 gttgttgcaa tggcatcata atcgagtggc aagagtgtgc gcaaatcaag tatatcggca
     5521 tcaatttgca attgagcaca agcttttttc gcccaatgca cacccatacc gtaagtaatg
     5581 atacttagtt catttccgct ttgtgccaat gctgctttac cgatttcaac cgtataataa
     5641 tcatcaggaa tattatcgct gatggcgcga tacaatgctt tgtgctcgaa aaacattacg
     5701 ggattcgggt cttcaatagc cgcgagcaac aatcctttcg catcataaac gcttgaagga
     5761 tataccactt tcaatccggg cgtatgaaaa aaccaggctt cattgctttg actatggaaa
     5821 ggtcctgcgc caacacctgc acccgtgggc atacgcacta cgctatcgac attttgtccc
     5881 cagcggtagt acatctttgc catgttattg ataatgggat tgaatccact cgttacaaaa
     5941 tcggcgaact gcatttcgat aacacttttc attttcttca ccgacagtcc gcaaccggcg
     6001 ccaagaattg cactttcgca aattggtgta ttgcggacgc gtgattttcc atactgctga
     6061 acaaagcctt cggttacttt aaatgcaccg ccatattctg caatatcctg acccataata
     6121 atgagttcag gatatttatc cattgccaat tttaacgcat cggaaatggc atttataaat
     6181 ttcttttgtg aagcaatatt tgatttcggc gctataactt cttgcgtaaa cggtgcataa
     6241 atatcgcgtt cttcctcttc ggtatcggga attacttccg gttcattaaa taccgtttgc
     6301 actcccgctt ctatttctga tttaatttca tcgcggaagg ttttaatcat ctcctcatta
     6361 ataatggccg actcgagtaa atattgttcg taattctgaa cgggatcttt tttaccccac
     6421 atttctatta attctttcgg cacatattta ataccacttg cttcttcatg tccgcgcata
     6481 cggaatgtca tacattctaa cagcaccggt ttttgttcat tgatacaata tgcgcgacat
     6541 tcttcgagtg cggcaaccat atccaaaata ttattgccat caacaatcat cgaacgcatg
     6601 ccataaccaa tcgccttatc cgcaatttgt gcgcagcgaa attgttcgtt ggtaggcgtt
     6661 gacaatccgt aaccattatt ttcaacaata aagattacag gcagatccca aacggcggca
     6721 acatttaatg attcatgaaa atcgccttcg ctagtagcac cttcaccggt gaatactgct
     6781 gttacttttt tctcgttttt caattttgcg gcaagtgcta tgccatcggc tactgccaac
     6841 tgaggtccca gatgcgatat cataccaacg atatgatact catttgtgcc aaagtgaaag
     6901 cttcgttcgc gacctttaga aaatccgttt ttctttcctt gccattgact gaataatttg
     6961 gcaaaaggga tgtcgcgacc ggtaaacacc ccaagatttc tgtgcatggg gaggatatat
     7021 tcgtcgtatt ccagcacata aggcacggca acggcaacag cctcctggcc aattccgctg
     7081 aaccacttac ttacttgtcc ctgacgcagg agattgagca ttttctcctc tatcatgcgg
     7141 ggtttgagca aacgcttata tatctgaatc agctgatcgt tgctgtaatg gcggcgatca
     7201 aactgtaaaa cgcgttggaa attagactgg gcggaatcca tggggcaaag ttacgacgaa
     7261 agagggagtg gaattagcaa attagctaat ggggggtgtg ctcgcatgaa gacgctcgtc
     7321 ttagttagca agtttgagag ttagcaagtg agcaagtgag caagtgaggg taattagcaa
     7381 gtgtgagagt tagcaaatta gcaaatgggg gtgaatgctt gcatgtttat ctctgcgact
     7441 ctgcggctct gcgtgaaaaa ggcgagaatc taaa
//
LOCUS       DAOQOU010000159         7423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5575_length_7423_cov_5.772937, whole genome shotgun sequence.
ACCESSION   DAOQOU010000159 DAOQOU010000000
VERSION     DAOQOU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_7423_cov_5.772937"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1106
                     /locus_tag="PLM90_11870"
     CDS             36..1106
                     /locus_tag="PLM90_11870"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ90122.1"
                     /translation="MAEQDDELELENIAQEGEEELYEHYRVTTDRGQEPLRIDKFLFN
                     RLINISRNRIQNAADAGCILVHGKPVKSSYKIKPFDEISVVLPYKPMKFDLKPEAIPL
                     TILFEDEDLLVVNKPPGLVVHPGLGNYSGTLVNALLYHFQGLPAPKDEPFRPGLVHRI
                     DKFTSGILVVAKQEYAMTHLAKQFFDHSIQRKYIALVWGEFDEPQGTIIGNIARHERF
                     RKMFAVYPPGGEIGKHAITHYRVLESFGYTSLIECQLETGRTHQIRVHMQHIGHPIFN
                     DDTYGGNRIVKGTVYTKYKQFVDNCFDLIPRQALHARSLGFVHPRSGEHLYFETPMPE
                     DMQAAVDKWRAYAQQLKQNGNV"
     gene            1153..2382
                     /locus_tag="PLM90_11875"
     CDS             1153..2382
                     /locus_tag="PLM90_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ90123.1"
                     /translation="MHRLFLFLILLISTSMHAQWCGTETIMDQIRIQQPETWARMQQI
                     RNEKAQGLRLDGEVYTIPVVVHIVYNNEVENIDDLRVYTQINILNEDFRRLNADTIDT
                     PAPFQGVAADVELEFCLASIDPNGDTTTGIIRTYSDTTEWLLTFSENVKYTEYGGDDA
                     WPASSYLNIWVCDLQHGILGYAVGPGGAAEIDGIVVDYSNFGLSDIGSAPYNLGRTAT
                     HEIGHWLGLTHIWGDDGNTCDGDDGIEDTPLQLGPTYGCPDFPLTDACSPDYPGIMFM
                     NYMDYTDDACMNMFTMGQKAKMLGVISTYRASILTSAAGCNDIGPLPGDLAELLVYPV
                     PSNGQFTVSVKNFAGTLDMMELHIYNTLGQHIASATLDATNNTAQYFDLSHLAPGTYV
                     VQAFNGSYFINKAFIIN"
     gene            2578..3873
                     /locus_tag="PLM90_11880"
     CDS             2578..3873
                     /locus_tag="PLM90_11880"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ90124.1"
                     /translation="MKKALLAGVLLASTSLMFGQAVDTRCGVHSYLNDLQTYFPEEYA
                     GIKAEAEKRQTVTTTYRTDGGVYTIPVVVHIVYSTDAQNIDDEFVYSQLDVLNADYRH
                     LNADAIETPAAFADLAADVMIEFCLAQQDPDGVITTGITRTETDISSWNLFVSPGAEN
                     YADNVKSSVKGGEDPWPRTDYLNIWVCNLTGGVLGYAMPPGGPSGKDGVVIGYKYFGN
                     DSPGGVYDKGRTATHEVGHWLGMAHIWGDDDFDPEPQCNGSDDMADTPNQEDATYGAP
                     SFPLLDDCAPASPGIMFMNYMDYVDDGSMNMFTEDQAAEMRNIMETTRESILSSQGCV
                     PGVDGINDVLKGQLIQLSVYPNPSSDIFVFEMKNFKNADMTVEVYNFSGELVDRVQLI
                     NNNGTATATFDASMLAAGTYMVKASDGDYILTQQITVVK"
     gene            3966..5162
                     /gene="mqnE"
                     /locus_tag="PLM90_11885"
     CDS             3966..5162
                     /gene="mqnE"
                     /locus_tag="PLM90_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HMZ90125.1"
                     /translation="MSDPRDIRILLHASSNDAELLEIADKVLTGERITPAEGIVLYEK
                     ASLGFAGTLADHVRTRMHGNIAYFNRNFHIEPTNLCVFDCKFCSYSRMLKQRSDGWVM
                     SKESMMDIVRSYADKQVTEVHIVGGVLPELNLEWFADVLSSIKAMRPDLHIKGFTAVE
                     LEYMIRKAKMSYRDGLAYLQKAGLDSLPGGGAEIFDETIREQICADKCDSATWLEIHR
                     CAHELGMHSNATILYGHIESFAHRIDHMERLRSMQDETKGFNCFIPLKFRNQHNQMSN
                     VEECSVVEDMRNYAIARIYLDNFPHIKAYWPMIGREQAQLSLHYGVNDIDGTIDDTTK
                     IYSMAGAEEQSPVMTTRQICELILAAGKQPVERDTLYNHIKSYSHESLDAEFGVDGFI
                     GLPVAN"
     gene            5191..5802
                     /locus_tag="PLM90_11890"
     CDS             5191..5802
                     /locus_tag="PLM90_11890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ90126.1"
                     /translation="MKKEIYIIRHGETDFNQLGIVQGRGVDSSLNETGRKQAQHFFDT
                     YKDAGFQKIFVSTLKRTHETIAPFRALGIPIIAHEGLDEISWGIHEGKKDGDTFKDFY
                     RVLHAWQHGETDLAIDDGESPITVQQRQLRFLDELTASGEDRILICSHGRAIRILMCT
                     MLKKPLSEMDTFPHQNTSLYKMIYDGNSFATDLFNDITHLHAT"
     gene            5792..6574
                     /locus_tag="PLM90_11895"
     CDS             5792..6574
                     /locus_tag="PLM90_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HMZ90127.1"
                     /translation="MQHKYSISLVSYLNTKPFLYGLQHEPEAAQFALELDIPSVGGKK
                     ILENQVDIGLVPVAVLAKLDDARIISDYCIGADGPVRSVCLFSHVPIDKIEKVFMDYH
                     SMTSVQLVNILLKEYWHVHPEQIPATTGYIEQIQGTTAGLIIGDRAIGLYDKFPYVYD
                     LSEAWKSHTGLPFVFAAWITRKEISADVLQSLNKALHLGVESIDAVVNMYAGNQLDKH
                     ALHDYFTLNIDYHLDGVKRKALELFIGKIRMYADVDGSLHEQ"
     gene            complement(6591..>7423)
                     /locus_tag="PLM90_11900"
     CDS             complement(6591..>7423)
                     /locus_tag="PLM90_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HMZ90128.1"
                     /translation="YHLHLFPEQVADIVITVGDPDRVAQVSKHFDAIEFQQQKREFIT
                     HTGTIGKKRITVISTGIGPDNIDIVLNELHALKQFDFKGHIAKSIEQPFTIIRFGTSG
                     SIQADIPVGTLLMSEMGIGIDNVMHFYNHRNNDAETAFLEQFKLHCYFHHTIHPYIAS
                     ADISLLTHFAKEFISGITLTAPGFYAPQGRAVNLPVSHPDFYSEITTFNHAGKRITNF
                     EMETATIYGLSALLGHRAISLNVLLANRATGEFITDPSAAVEHLIKTGIEMIADSDFK
                     "
BASE COUNT         2178 a         1476 c         1645 g         2124 t
ORIGIN      
        1 tattttgaaa tcgtttttga ttgtaaatta gtgtaatggc agagcaggac gacgaactcg
       61 aattagaaaa tattgctcag gaaggtgagg aagaattata cgaacattat cgtgtaacta
      121 ccgatcgtgg acaagaacct ctgcgcatcg ataaattttt attcaaccgg ctcatcaaca
      181 tctcccgaaa ccgcattcaa aatgctgctg atgcgggatg tatccttgtg catggaaaac
      241 cggttaagtc cagttataaa ataaaacctt tcgatgaaat atcggtagtg cttccgtaca
      301 agccgatgaa attcgatctc aagcccgagg caattccact taccatatta tttgaggatg
      361 aagatttact ggtggtgaat aaacctcccg gtcttgttgt gcatccgggt cttggaaatt
      421 attccggcac tttggtcaat gcattgttgt atcattttca aggattgcct gcaccaaagg
      481 atgaaccttt tcgtccggga ttagtgcacc gcattgataa atttacatca ggcatacttg
      541 tagtggcgaa acaggaatat gctatgacgc atcttgctaa acaatttttc gaccacagca
      601 tacaacgaaa atatattgct ttggtgtggg gagaatttga tgaaccgcaa ggaaccataa
      661 taggaaatat tgcacgtcat gaacgcttca gaaaaatgtt tgcagtatat ccacccggag
      721 gcgagattgg aaaacatgcc atcacacatt atcgcgtatt ggaatcattt ggttatactt
      781 cattaatcga atgccagctt gaaaccggtc gcacgcatca aatccgtgtg cacatgcaac
      841 atatcggtca tccgattttc aacgacgata cttatggtgg taaccgcatt gtaaaaggta
      901 ctgtgtatac caaatacaag cagtttgtcg ataattgttt cgaccttatt ccgcgacaag
      961 cattacacgc acgttcgctg ggctttgtgc atccgcgatc aggagaacat ttgtatttcg
     1021 aaacaccaat gcctgaagat atgcaggcag cagtagataa atggcgtgcc tatgcgcaac
     1081 aattaaaaca gaatggaaac gtttaataag ctccgtgaac accatcaacc taaaacgact
     1141 atctttattc ccatgcaccg tctgtttcta tttttgattt tattgatttc tacatccatg
     1201 catgcccaat ggtgtggcac agaaaccatc atggatcaga ttcgcataca gcaaccggaa
     1261 acctgggcac gcatgcagca gatcaggaat gaaaaagcgc aaggtcttcg tctcgatggt
     1321 gaagtgtata ctattcccgt tgtagtgcat attgtttaca ataatgaagt agaaaatatc
     1381 gacgacctgc gggtttacac gcaaataaat atattgaatg aagatttcag acgattaaat
     1441 gccgatacca tcgatacacc tgcaccattt cagggagtag ccgctgatgt tgaattggaa
     1501 ttttgtctgg catcaattga tccaaatggc gataccacaa ccggtatcat tcgcacctat
     1561 tccgatacta ctgagtggct gttaaccttt tcggaaaatg taaaatacac ggaatatggc
     1621 ggtgatgatg catggccggc ttcctcatac ttgaatatct gggtctgcga tcttcaacac
     1681 ggtattttgg gctatgctgt cggtcccggc ggcgctgctg aaattgatgg tatcgtggtc
     1741 gattattcca acttcggact atcagatatc ggttccgcac cgtacaacct cggccggact
     1801 gcgacacatg agatcggcca ttggctcgga cttacacata tttggggtga tgatggcaat
     1861 acttgcgatg gcgatgatgg cattgaagac acgccgcttc aacttggccc tacttacgga
     1921 tgtccggatt ttccacttac ggatgcttgt tcaccggatt atcccggcat catgttcatg
     1981 aattatatgg attataccga tgatgcttgt atgaatatgt ttaccatggg tcagaaagct
     2041 aaaatgcttg gtgttatctc cacttacaga gcatccattc ttacatcggc tgccggttgt
     2101 aatgatatcg gtccattacc cggagatctg gcagaactcc tggtttatcc tgtgccttca
     2161 aacggtcagt ttacagtgag tgtaaagaac ttcgccggta ccctcgatat gatggaactc
     2221 catatctaca acacacttgg gcaacatata gcttctgcca ctctcgatgc tacaaacaat
     2281 acggcacagt atttcgacct ctcgcacctg gctcccggaa cctatgtggt acaagccttt
     2341 aatggctcgt atttcataaa taaagcgttc attatcaatt aattacaatt ttgtacagct
     2401 gaaaacgggt ctcggagaat cgagtcctgc tactttacat gcaaacggta tgccgatttt
     2461 gaagtttaag gtgcagaaat ggcggagtgt tgacagtatt gaacaagtta cttttaacaa
     2521 tatgcgttca tcttgttaac tttgttatct aatcagtaac caatccacga caaacaaatg
     2581 aaaaaagctc tactcgcagg tgtcctcctg gcaagcacaa gtttaatgtt tggtcaggcc
     2641 gttgacacac gttgcggtgt tcacagttac ctgaatgacc ttcaaactta ttttccggaa
     2701 gaatacgccg gaattaaagc agaagctgaa aagcgtcaaa ccgttaccac tacttacaga
     2761 acagatggcg gtgtttatac catcccggta gtagtgcata ttgtatattc taccgatgca
     2821 cagaatattg atgatgaatt tgtgtattca caattagatg tgttgaatgc cgactatcgt
     2881 catttaaatg ctgatgctat tgaaactccg gcggcttttg ccgatttggc agctgacgtg
     2941 atgattgaat tttgtcttgc acaacaggat cctgatggtg taatcactac cggtatcacc
     3001 cgcacagaaa cagacatatc atcatggaat ttatttgttt ctcccggagc tgaaaattat
     3061 gctgataacg taaaaagttc agttaaaggt ggtgaagatc catggccacg aaccgattat
     3121 ttaaatattt gggtttgtaa tctcaccggt ggggtattag gatatgcaat gcctccggga
     3181 ggaccttcag gaaaagatgg tgtggtgata ggatacaaat attttggcaa tgacagtccg
     3241 ggtggcgttt acgacaaagg tcgtactgca acacacgagg ttggccactg gttaggaatg
     3301 gcgcatattt ggggtgatga tgacttcgat ccggaaccac aatgtaacgg ctcggatgat
     3361 atggctgata cgccaaacca ggaagatgcc acttatggtg caccaagttt cccattgctc
     3421 gatgattgtg ctcctgcttc tcccggcatt atgtttatga actacatgga ttatgttgat
     3481 gatggttcta tgaatatgtt tacagaagat caggcagcag agatgcgcaa tatcatggaa
     3541 actacgcgcg aatctattct ttcatcgcaa ggttgtgtgc ccggtgttga tggaatcaat
     3601 gatgtattga aaggccaatt gatccaatta agtgtttatc ctaacccttc cagcgatatt
     3661 tttgtatttg aaatgaaaaa tttcaaaaat gcagatatga ctgttgaagt atataatttc
     3721 agcggcgaat tagttgaccg tgtacaatta ataaacaaca acggaacagc aactgctact
     3781 ttcgatgctt ctatgctcgc tgcaggcact tacatggtga aagcaagcga tggtgattat
     3841 attctgacac aacagataac agttgtgaaa taatatttat taaatgaact tatctttcaa
     3901 aaaaagtggt tgatctttca gccacttttt ttgttgtatc caacgtaaac aatcaacttt
     3961 gttccatgag cgatccacgt gatatccgga ttttgttgca cgcctcttcc aatgatgccg
     4021 aattgctgga aattgcggat aaagtattga ctggcgaacg catcacgccg gcagaaggta
     4081 ttgtattgta tgaaaaagct tcactcggct tcgcgggcac tctggcagac catgtgcgca
     4141 cacgtatgca tggcaatatc gcctacttca ataggaactt tcacatcgaa ccaactaacc
     4201 tttgcgtgtt cgattgcaaa ttttgcagct attccagaat gctcaaacaa cgcagtgatg
     4261 gttgggtgat gagcaaagaa tccatgatgg acattgtgcg ctcttatgcc gataagcagg
     4321 ttacagaagt gcatattgtt ggcggtgtat tacctgaatt aaatcttgaa tggttcgcag
     4381 atgtgctgag cagcataaaa gcaatgcgcc ccgatttgca tatcaaaggt tttactgccg
     4441 ttgaattgga atacatgatc cgcaaagcaa aaatgagcta tcgcgatggt cttgcttatc
     4501 tccaaaaagc cggattagac agccttcccg gtggtggtgc agaaatattt gatgaaacta
     4561 tacgtgaaca gatctgcgcc gataaatgtg atagcgccac ctggctcgaa atacatcgtt
     4621 gtgcgcacga attaggtatg catagtaatg caacaattct atatggtcat atcgaaagtt
     4681 ttgctcatcg cattgatcat atggagcgat tgagaagcat gcaggatgaa acaaagggat
     4741 tcaattgttt tattcctttg aaattccgca atcagcataa ccagatgagc aacgttgaag
     4801 aatgtagtgt tgtagaagac atgcgcaatt atgcaattgc ccgtatctat ctcgataatt
     4861 tcccgcacat aaaagcatat tggccaatga taggtcgcga acaggcgcaa ttgagtctgc
     4921 attatggtgt aaatgatatc gatggtacga ttgatgacac aacgaaaatt tatagcatgg
     4981 caggcgctga agaacaatcg ccggtaatga ccacacgcca gatatgtgaa ttgatattag
     5041 ctgcaggaaa gcaacctgtc gaacgcgata cactttacaa ccatattaaa tcatacagtc
     5101 atgaatcatt ggatgccgaa tttggtgttg atggatttat cggattgcct gttgcgaatt
     5161 aataccaatt attaaatctg cttctttact ttgaaaaaag aaatctatat catccgacac
     5221 ggcgaaacag atttcaatca attgggaatt gtgcagggca ggggagtgga tagttcactc
     5281 aacgaaacag gcagaaagca agcgcaacat tttttcgata catacaagga tgccggtttt
     5341 caaaaaatat tcgtcagcac actcaagcgc acacatgaaa caatagcgcc attccgcgca
     5401 cttggcatac cgattattgc tcatgaagga ctggatgaaa taagctgggg aattcatgaa
     5461 ggcaagaaag atggtgatac cttcaaagac ttttatcgcg tattacatgc ctggcagcac
     5521 ggtgaaacag atttggcaat tgatgatggc gaaagcccga tcactgttca gcagcgacaa
     5581 ttaaggtttt tagatgaatt gacagcaagc ggagaggatc gcatcctgat ttgcagtcat
     5641 ggtcgcgcta ttcgcattct gatgtgcaca atgctgaaga aacctttatc ggaaatggat
     5701 acctttccgc atcaaaacac cagtttatat aaaatgatct acgatggcaa tagctttgcc
     5761 accgatttgt ttaacgatat tacgcatttg catgcaacat aaatacagta tatcattagt
     5821 cagttatctg aataccaagc catttcttta cggattacag cacgaacccg aggcagcaca
     5881 gtttgcgctt gaattggata ttccatctgt tggcggaaaa aagattttgg aaaatcaagt
     5941 agatatcgga ttggtgccgg ttgcggtatt agcaaaactt gacgatgcac ggatcatttc
     6001 agattattgt ataggtgccg atggacctgt tcgcagtgta tgtttgttta gtcatgtgcc
     6061 tatcgataag atagaaaaag tattcatgga ttatcattct atgacttcgg tacaattagt
     6121 aaatatcttg ttgaaggaat attggcatgt gcatcctgaa caaattccgg ctacaacagg
     6181 atatattgaa caaattcaag gcacaactgc cggcttgatc attggcgatc gcgcaattgg
     6241 gttgtatgat aaattccctt atgtatatga tctgagtgaa gcatggaagt cgcataccgg
     6301 tttgccattt gtattcgctg catggatcac cagaaaagaa atttctgctg atgtattgca
     6361 atcactcaat aaagctttgc atttaggggt ggaaagtatt gatgcagtag tgaatatgta
     6421 tgccggcaat caattggata aacatgcatt gcatgattat tttaccctta atatcgatta
     6481 ccaccttgat ggtgtaaaac gaaaagcatt ggaattattt atcggcaaaa tcagaatgta
     6541 tgccgatgtt gatgggtcac tccatgaaca ataaagccgc gatagataat ttatttaaaa
     6601 tcgctatctg caatcatttc aattcctgtt ttaatcaggt gctctacagc tgccgatggg
     6661 tctgtaataa actctcccgt tgcacgattg gcgagcaata catttaaaga tatcgccctg
     6721 tggccaagca aggcagaaag tccataaatg gttgcagttt ccatttcgaa gttggtgatg
     6781 cgtttgccgg cgtggttgaa agtggtgatc tccgaataaa aatccggatg actcaccggt
     6841 aaattcacgg cgcgaccttg aggagcataa aatcccggtg cggtgagtgt gatgccggaa
     6901 atgaattctt ttgcaaaatg agtcaacagt gagatgtcgg cgcttgctat gtagggatgt
     6961 atcgtatgat ggaaatagca gtgtaattta aattgttcaa ggaaagctgt ttctgcatca
     7021 ttgttccggt gattgtaaaa gtgcatgaca ttatcgatac caattcccat ttccgacatc
     7081 aataatgtgc caacaggtat atcggcttga atggaacccg aagtgccgaa cctgatgatg
     7141 gtaaatggct gttcaattga ttttgcaata tgacctttga aatcaaattg ttttaatgca
     7201 tgtaattcat ttaatacgat atcaatatta tcaggaccaa tacctgtact gattactgta
     7261 attctttttt tgccgatagt cccggtatgt gtgatgaatt ctcttttctg ttgttgaaat
     7321 tctattgcat caaagtgctt gctaacctga gcaaccctgt cgggatcgcc aacggtaatt
     7381 acgatgtctg caacctgttc ggggaacaaa tgcaagtgat aga
//
LOCUS       DAOQOU010000160         7411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5595_length_7411_cov_6.103134, whole genome shotgun sequence.
ACCESSION   DAOQOU010000160 DAOQOU010000000
VERSION     DAOQOU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_7411_cov_6.103134"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2150)
                     /gene="recG"
                     /locus_tag="PLM90_11905"
     CDS             complement(24..2150)
                     /gene="recG"
                     /locus_tag="PLM90_11905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMZ90129.1"
                     /translation="MASLNLQTPLTFLKGVGEQRAAMLLKELELETCYDLLHFFPFRY
                     IDRTDFTTVKAVKQNAFTLAELREVQMKGFISKMHIVGAKQGRRLIATFSDNTDTMEL
                     VWFNGINWLQKSLKEDVQYIVYGKPSIYNGVVNMMHPEMEPAAQSDFQLSGKIQPIYS
                     GTEKLKSRGINSKMIARFVQSLIAQLSELDLPEQLPASVIQKYKLIGRFEAYKCIHFP
                     DSDAHLQHALRRLKFEELFIDQIKLLRVKQYRHSSSMGFIFKELDTYFNPFYTAHMPF
                     ELTNAQKRVMKEIRKDLLSGRQMNRLLQGDVGSGKTVVALLTMLMALDNGFQACLMAP
                     TEILSQQHIAGISELLKDLPIQVALLTGSVKGKQRKLILEGVADGSIQILIGTHALIE
                     ETVQFKNLGCVVIDEQHRFGVEQRSKLWNKNSLTPHILVMTATPIPRTLSMAYYGDLD
                     VSIIDELPPGRKPIKTVHRKESERLHVFGFLKEQIAEGRQVFIIYPLIEESEKLDLKN
                     LMAGAEAIERAFPKPQYQVSILHGKMKPADKDFEMQRFLNKQTHIMISTTVIEVGVNV
                     PNATVMVIENAERFGLAQLHQLRGRVGRGAHQSFCLLMTDVKLSNDARKRISTMVETN
                     DGFKIAEVDMQLRGPGEIEGTRQSGAPQFKIANLQTDEPILREARLTADEILDADPNL
                     NTPEHAALKKFLSAQSWKIKQWGKVS"
     gene            complement(2255..2848)
                     /locus_tag="PLM90_11910"
     CDS             complement(2255..2848)
                     /locus_tag="PLM90_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90130.1"
                     /translation="MSLFRTVIFCFALAAIIGCHTDEAPKPDAWPRVHYPVATDTIVF
                     NDEDCPYTFSLPNYYEIDRNVTYFNQTPDDPCWMNLVCKDLNATIYMSNKPIDVKYSY
                     QQMIDDAYKLTYKHAGRADYIEPQEIENQYGVHGLIYYVGGDAASNFQFFVTDTTSNF
                     VRGALYFNNRPNADSLRPVIQFMVHDIENMLSGWRWK"
     gene            complement(2883..3302)
                     /locus_tag="PLM90_11915"
     CDS             complement(2883..3302)
                     /locus_tag="PLM90_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ90131.1"
                     /translation="MSGVNKVILIGNLGKDPELRNFEGGNVVANFTLATTEVFRDRNG
                     NKVEQTEWHNIAMWGKLAEIAGKILRKGAKVYIEGRIKNRSWEDKEGHKKYITEIVAD
                     NFTLLGPRPANTGGIDHPDDDMNHQHPQSDIQDDLPF"
     gene            complement(3402..4451)
                     /gene="mutY"
                     /locus_tag="PLM90_11920"
     CDS             complement(3402..4451)
                     /gene="mutY"
                     /locus_tag="PLM90_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HMZ90132.1"
                     /translation="MSSFSKTLLQWYRLNKRNMPWVGETDPYKVWLSEVILQQTRVEQ
                     GTAYYLDFIKHYPTVQHLAKASANEVMKRWEGLGYYSRARNMHATANVITQQLKGVFP
                     DTPEGLQSLKGIGKYTANAILAYAYHKPYAVTDGNVLRILSRYQGITIPVNTPAGKKM
                     VEALADRLLDKQLPHTYNQALMDFGSLVCTPRNPDCGNCPFRRSCYAFNHEMTAILPQ
                     KEKKRAAKTRYFNFFVMKSGNKVLIMPRHAADIWKALWQFPLIETAEEATLQDIQRSN
                     VYKDWKLGKVQWEEAGSFKQQLTHRRLICRFFTGEIKTTPEIDGMQELVNRKEMERFA
                     FPGVIRDFMKGKLYF"
     gene            4557..4856
                     /locus_tag="PLM90_11925"
     CDS             4557..4856
                     /locus_tag="PLM90_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ90133.1"
                     /translation="MRKADIVNRITDKTGLEKVDVMVVVEAFCKEIKESMAEGHNVYL
                     RGFGSFVVKTRKKKIGRIIKKNIAIEIPEQKIPSFKPAKLFLNRVKDGKKSKSGL"
     gene            4857..5645
                     /locus_tag="PLM90_11930"
     CDS             4857..5645
                     /locus_tag="PLM90_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90134.1"
                     /translation="MKKYLIPAGIAVVLILVIFFAFDHKPPKVSVFDERREKLIDSLQ
                     YPNSEDLMHMHRDMLHAGGGTKAALLQQLADKWMQLAQPSIAADYIRQLADADPSYEH
                     YMQAGAAMGSLIDFEAEEDLRMSLVYGARYSYEMAEKLQPGDLDARIGLASVLVSGGS
                     TPMEGIMMLREMDAENPDNIRINLELGKFSVMSGQFDKALERFDRVLQKDSLNLQARY
                     MRAQSFLGIQDTAKAVMELETLKSQTTDPTLIEQVDNEIKNLNH"
     gene            5853..7406
                     /locus_tag="PLM90_11935"
     CDS             5853..7406
                     /locus_tag="PLM90_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HMZ90135.1"
                     /translation="MERELIINTTPTGAEIALLEDKQLVEIHRDSFSSRFNVGDLYLG
                     RVKKITPGLNACFVDVGFERDAFLHYTDLNPQFKSIYKYTKAAQDGRPYSMDKFQREP
                     DIVKTGKINNVISKSQHILVQIIKEPIQTKGPRLSCEISLPGRYLVLTPFNEVVGVSR
                     KIASGEERKRLQKAIEQLRPKGFGVIIRTVAENVPVQKLHDDLNNLVNQWKEITNRLQ
                     SAQAPQLIFSESKKTNTLLRDVLNDSFNRVVVNDNAIQTEVKDYIRRISPGSEKMVLM
                     HSGPKSVFEQFGVTRQIKGLFGRTVNMDSGAYLIIEHTEALHVIDVNSGNKSAVKGDQ
                     EANAVSVNMEAAREIARQLRLRDLGGIIIVDFIDMKNPENKKAVYNALKNAMAGDRAK
                     HTILPISKFGVAQITRQRVRPEVNISTAEVCPTCNGTGKIEASILLLEDIERKLKHLV
                     TIQNLKAVNLMVHPYVAAYLKKGWFFRSLYWQWRRTYKVKLKVTSSDDFHYMEYRFFD
                     LNEGEINVE"
BASE COUNT         2019 a         1560 c         1652 g         2180 t
ORIGIN      
        1 ttttcctctc tccttcttcc tatttaagaa acctttcccc attgcttaat tttccaactc
       61 tgggctgaga ggaatttttt cagtgccgcg tgttcggggg tgtttagatt tgggtcggcg
      121 tcgaggattt cgtcggccgt aagtcgcgct tcgcggagga tgggttcgtc ggtttggaga
      181 ttggcgattt taaattgcgg tgcaccactt tgtcttgtgc cctctatctc accgggaccg
      241 cgcaattgca tgtcaacttc cgcaattttg aaaccatcgt ttgtttctac catggtgctg
      301 attctttttc gtgcatcatt actcaatttc acatcagtca tcaacaaaca aaacgattga
      361 tgtgcacccc ttccaactct tccacgcaat tgatgtaatt gtgccagtcc gaagcgttcg
      421 gcattttcta tcaccatcac agttgcattc ggcacattga ctcccacttc aataacggtg
      481 gtggaaatca tgatatgtgt ttgcttattt aaaaaacgct gcatctcgaa atctttatcc
      541 gcaggtttca tttttccgtg taatatgctc acttgatatt gcggttttgg aaatgcccgt
      601 tcaatggctt cggcacccgc cattaaattt ttcaaatcga gtttttcact ctcttcaata
      661 agtggataaa taataaatac ttgccgacct tcggcaattt gttctttcag aaatccaaat
      721 acatgcaagc gctcactctc ttttcgatgc actgttttta tcggcttgcg acccggtggg
      781 agttcgtcga tgatgctaac atccagatca ccataatagg ccatggaaag tgtgcgggga
      841 ataggtgttg ccgtcatcac cagaatatgc ggcgtaaggc tgtttttatt ccacaatttc
      901 gatcgttgct ccacaccaaa tcgatgttgc tcatctatta caacacaacc aagattttta
      961 aattgaactg tctcttcaat taatgcatgt gtgcctatta aaatctgtat actgccatcg
     1021 gcaacacctt ccagaattaa ttttcgctgc ttgcctttca cactgcccgt aagtaatgca
     1081 acctgaatgg ggagatcttt taatagttct gatatccctg ctatatgttg ttgcgataat
     1141 atttctgtag gcgccattaa acaagcctga aatccattgt caagtgccat caacatggtg
     1201 agcagtgcta ccactgtttt accgctaccc acatcgccct gcaataatcg attcatttgt
     1261 cggccactca gcagatcttt tctgatttcc ttcatcaccc ttttctgtgc attggtgagt
     1321 tcgaagggca tgtgtgctgt gtaaaacgga ttgaagtatg tatctaattc cttaaaaata
     1381 aatcccatgc tgctgctatg gcgatattgt tttacgcgca gcaattttat ctggtcgata
     1441 aataattctt caaatttcaa tcgccgcaag gcatgttgta aatgcgcatc ggaatcggga
     1501 aaatgtatgc acttgtatgc ttcaaatcga cctatcaatt tatatttctg aatgacggaa
     1561 gcgggcaatt gttcaggcaa atctaattcc gataattgtg ctatcagcga ctgcacaaat
     1621 cgggctatca ttttactatt gataccgcga ctttttaatt tttcggtacc tgaatatata
     1681 ggctgaattt ttccactgag ttgaaaatca ctttgtgctg caggctccat ttcgggatgc
     1741 atcatattta ctacgccatt ataaatggaa ggctttccgt aaacaatata ttgcacatcc
     1801 tccttcagcg atttctgcaa ccagttgatg ccattgaacc ataccaactc catggtatcg
     1861 gtattgtcgc tgaaagtggc aatcaatctc cgcccttgtt tagcaccaac aatatgcatc
     1921 ttgctgataa accctttcat ctgcacttcg cgcaattcag ctagcgtgaa ggcattttgc
     1981 ttaaccgctt taacagtagt aaaatcggtg cgatcgatgt atctgaacgg aaaaaaatgc
     2041 agcaaatcat agcaggtttc caattccagc tccttcagca acattgctgc acgttgttcg
     2101 cccacgcctt tgagaaaggt gagtggtgtt tgcagattca ggctagccac gagataattt
     2161 aagcaatcgg aaagttagca aataaaataa ttattgaagt ttagtatcgt caatcaataa
     2221 ttatgcagca cacatttgtt atcgcccaca aacatcactt ccacctccaa ccgctcaaca
     2281 tattttcaat atcgtgcacc ataaattgaa tgacgggtct taatgaatct gcatttgggc
     2341 ggttgttgaa gtataatgct ccacgaacga aattgctggt ggtatcggtt acgaaaaatt
     2401 gaaaattact cgctgcatca ccaccaacat agtagatcag tccatgtaca ccatactgat
     2461 tttctatttc ctgtggttcg atataatcag ctctgcctgc atgtttgtat gtgagcttgt
     2521 aggcatcgtc gatcatctgt tgataggaat attttacatc aataggttta ttgctcatgt
     2581 aaatagtggc attcaaatcc ttacacacta aattcatcca acacggatcg tcaggtgtct
     2641 gattgaaata agtaacattg cgatctattt cgtaataatt tggaagtgaa aatgtataag
     2701 ggcaatcttc atcattaaac actatcgtat ccgttgcaac aggataatgc actcttggcc
     2761 aggcatcggg ttttggcgct tcgtcggtgt gacagccaat aattgctgcc agtgcaaagc
     2821 agaaaattac cgttcggaat aatgacatct tgtaaagatc gctattaaat taggattgca
     2881 gatcaaaatg gcagatcgtc ttgtatgtcg gactgcggat gttgatgatt catatcgtca
     2941 tccggatgat caatgccacc ggtgtttgca gggcgtggtc ctaataaggt gaaattatcg
     3001 gcgacaattt cagtgatata ttttttatga ccttccttgt cttcccaact gcgattttta
     3061 attcgccctt cgatataaac tttcgcgcct tttcgcaaaa tctttcctgc aatttctgca
     3121 agcttccccc acatggcaat attatgccat tcggtttgtt ctactttatt gccattgcgg
     3181 tcgcggaaaa cttctgtggt tgcgagtgtg aaattcgcca ctacattgcc gccttcgaaa
     3241 ttgcgcaact ccggatcttt ccccaggttg cctatcaata tcactttgtt tacaccactc
     3301 atgatgttct gtttacgtat ctgttacact taatttcgct tcaaaaataa cgttggggac
     3361 aggaatagaa aaacactgat ggatggagca tatttccaat attaaaagta cagctttccc
     3421 ttcataaaat ctcttattac ccctggaaaa gcaaatcttt ccatttcctt tctgttcacc
     3481 aattcttgca tgccatcgat ttcgggagtg gtttttattt ctcctgtaaa aaatcggcat
     3541 atcaatcgcc ggtgggtcag ttgttgtttg aaacttcctg cttcttccca ttgcaccttc
     3601 cccagcttcc aatctttata aacgtttgaa cgctgaatat cttgtaaagt tgcctcttct
     3661 gctgtctcta taagtggaaa ttgccacaat gccttccaga tatccgctgc atgtcggggc
     3721 atgatcagca ccttgttacc cgatttcatc acaaaaaagt taaaatatct cgtttttgcg
     3781 gcccgtttct tttctttttg ggggaggatg gcggtcatct cgtggttgaa ggcataacag
     3841 gaacgccgga aggggcagtt tccgcaatca ggattccgcg gtgtgcacac cagcgagccg
     3901 aagtccatca gtgcctggtt gtaagtatgt ggtaattgct tatcgaggag cctgtcagca
     3961 agcgcttcca ccattttttt tcctgcaggt gtattcacgg ggatggtgat tccctgatac
     4021 ctggagagta tgcgtagcac atttccgtca gttacggcat agggtttgtg gtatgcatag
     4081 gcgaggatgg cattagccgt gtattttcct atgcccttga ggctttgaag tccttccggg
     4141 gtgtcgggga aaacgccttt taattgttgg gtgatgacgt tggcggtagc atgcatgttt
     4201 ctggcgcggg agtagtatcc caggccttcc cagcgtttca ttacctcatt ggccgacgct
     4261 tttgcaaggt gctgaacggt agggtagtgt ttaatgaagt cgagatagta ggctgtccct
     4321 tgttccacgc gggtctgctg caaaatcacc tcactcaacc acaccttgta ggggtcagtt
     4381 tccccaaccc agggcatatt gcgcttgttg aggcgatacc attgaaggag cgttttggag
     4441 aaagacgaca tgcgggtgca ataatacaaa gggaaaaaaa tgtgtggtta ttttataata
     4501 attaagcgaa ctttgcagtt taacagattg attatcaaac caaaagaaat atacctatga
     4561 gaaaggctga tattgtaaac cgcattaccg acaagacagg tttggagaag gtggatgtaa
     4621 tggttgtggt ggaagcattt tgtaaagaga tcaaagaatc catggctgaa gggcacaacg
     4681 tgtatctgcg tggtttcggc agttttgtgg tgaagacacg taagaaaaag atcggccgta
     4741 tcatcaaaaa gaatattgct atagagatac ccgaacaaaa aatcccctct ttcaagccgg
     4801 ctaagttgtt tttaaaccgc gtaaaagacg gtaaaaagag taaatcaggt ctctaagtga
     4861 aaaagtatct tattcctgcc gggatcgccg tggtgctgat tctggtgatt ttcttcgcat
     4921 ttgatcataa gcctcctaag gtttctgttt tcgacgaacg tcgtgaaaag ctcattgatt
     4981 cattgcaata tcccaattct gaggatttga tgcacatgca tcgcgatatg ttgcatgccg
     5041 gtggtggaac gaaggcggcg ttgttacagc aattggcaga taagtggatg cagctggccc
     5101 agccttcgat agcggcagat tacatcagac aacttgccga tgcggatcca tcttacgaac
     5161 attacatgca agccggcgct gcaatgggct cgctgattga ttttgaggct gaagaagacc
     5221 tgcgtatgag ccttgtttac ggggcgcgat atagttatga gatggcagaa aagcttcagc
     5281 ccggtgattt ggatgcacgt atcggactgg catcggtgct ggtatcgggt ggtagcacgc
     5341 caatggaagg tattatgatg ctgcgggaaa tggacgccga gaaccccgat aacatcagaa
     5401 ttaacctcga attgggcaaa ttttcggtca tgagcggaca gtttgataag gcattagaga
     5461 gatttgatcg tgttttacaa aaagattccc taaatttgca ggctcgttac atgagggctc
     5521 agtcgtttct tggaattcag gacaccgcaa aggccgtaat ggagctagaa acactgaaat
     5581 cgcaaaccac cgacccgacc ctgatcgaac aggtggataa tgagatcaaa aacttaaatc
     5641 attaaaaaat atttgttatg ccttccggta agaaacgtaa aagacataaa atggctactc
     5701 acaagcgcaa aaagcgtttg aggaagaacc gccacaagaa aaagtaatcc aaccggcttt
     5761 ttccgtacct gtatattctc ctaccttttc cagccccaac aggggtaata actttataac
     5821 atcccataaa gggagcaaac ccgtatttga acgtggagag ggaacttatt attaatacca
     5881 cccccacggg tgccgagatt gcattactcg aagacaagca gttggtagag atacatcgcg
     5941 actcgtttag cagccgtttt aatgttgggg atctatatct cggtcgtgtg aaaaaaatca
     6001 cgccgggact caatgcctgt tttgtcgatg ttggtttcga acgcgatgcc tttttgcatt
     6061 ataccgatct caacccgcaa ttcaagtcta tctataaata caccaaagct gctcaggatg
     6121 gccggccgta tagcatggac aagttccagc gcgagcctga tatcgtgaaa acggggaaga
     6181 tcaataatgt gatctccaag agtcagcata ttctggtgca aattatcaaa gagcctattc
     6241 aaaccaaagg tccgcgtttg agctgtgaga tttctctgcc gggaagatat ttggtgctga
     6301 cgccctttaa tgaagtagtt ggcgtttcgc gaaaaattgc cagcggagaa gaacgcaaac
     6361 gcttgcaaaa agcaattgaa caactgcgac caaaaggttt tggtgtcatc attagaactg
     6421 ttgccgaaaa tgtacccgtt caaaagctgc acgacgattt aaataatctc gtcaatcagt
     6481 ggaaagagat caccaatcgc ttacagagtg ctcaggctcc gcagttaatt ttcagcgaat
     6541 cgaaaaaaac gaatacgctt ctgcgagatg tactgaatga ttcttttaat cgcgtagtag
     6601 taaatgataa tgcgattcaa accgaggtaa aagattatat tcgacgcatt agtcccgggt
     6661 ccgaaaaaat ggtgttgatg cacagcggac caaaaagtgt gtttgaacaa tttggcgtta
     6721 cacgtcagat aaagggattg tttggtcgca cggtgaatat ggatagcggt gcatatttaa
     6781 ttattgagca tacggaagca ttgcatgtga ttgacgtgaa tagcggaaat aaatctgctg
     6841 ttaaaggcga tcaggaagcg aacgcagttt cagtgaatat ggaggctgcc agagagattg
     6901 ctcgtcagtt gcgattgaga gatctcggcg gaattattat tgtcgatttt atcgacatga
     6961 aaaatccgga aaataaaaag gctgtttaca atgcattgaa aaatgcgatg gcgggggata
     7021 gggcgaagca cactatctta ccgatttcga aattcggtgt tgcacaaatt acacgccagc
     7081 gtgtgcgtcc ggaggtgaat atttctactg ctgaagtttg tcctacatgt aatggaacgg
     7141 gtaaaattga agcgagtatt ttattgctgg aagatattga gcgcaagctg aaacatcttg
     7201 ttaccataca aaatctgaaa gcggtgaatc ttatggtgca tccttatgtg gctgcatatc
     7261 tgaaaaaagg ttggttcttc cgcagtttat attggcagtg gcgacgtact tacaaagtaa
     7321 agcttaaggt tacatcgagt gacgattttc attatatgga atatcgcttc ttcgatttga
     7381 atgaagggga gataaacgtg gagtaggaga g
//
LOCUS       DAOQOU010000161         7284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5754_length_7284_cov_5.903721, whole genome shotgun sequence.
ACCESSION   DAOQOU010000161 DAOQOU010000000
VERSION     DAOQOU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5754_length_7284_cov_5.903721"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2659)
                     /locus_tag="PLM90_11940"
     CDS             complement(<1..2659)
                     /locus_tag="PLM90_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HMZ90136.1"
                     /translation="MKTLYLKSLFLLALVTSGSLYGQSLAMDWQKTIGGTDYETADIV
                     LMTPDHGFIFGSSSLSGISAYKSEPSYGGADYWIIKTDSAANVEWETTIGGSGDDYLN
                     YLSLTDDGGYILGGTSNSDISGVKTSNTIGPYGNPDAWVVKLNSTGHIEWQKVFGGGY
                     DDFVGSINQTPDGGYILGANKEQAGSFIYNYNVYKLNNIGTIEWQREYYAFWDDQLVR
                     AIQSDDGGYLLCGYSNSDITGDKTEPMIDVTDFWIIKINSIGDIEWQNVIGGTHFDNC
                     VDAIKTADGGYLLAAESQSPAGYDKSENSIYYYDYWVVKINATGEVVWENTIQGNNLD
                     YLRCIIQAQDGGYMLGGKSASSIGYDKNSNTYGDYDYWIVKTDESGNILWQKVFGGTN
                     DDDLTSMTITNDGGVLLSGSSRSIVTGNKTAPNLGNYDLWLIKLTPDFSTISGTLYAD
                     LNANTLHDAGEPPLTYKPVYISPEGLTEFSDSSGYYAFQVTSLDTFSYSSDTLPYYTS
                     VPANYTTVIDSFYTVDSLRNFGFQPDSVMNALVIDITPIYLFRPGATVKYTLSYQNIG
                     TTSINGQINLIYDPLLSFMSADLAGYTLYDDSITWSLGTIAPFESGLITVTFIVSETA
                     DAGDMVTSSALIYSPDVYSIVDNSISNSVNEIIASYDPNNMIVNLVELTPEELAVTDH
                     LNYTINFQNIGTDTAFNIIVNNILPVGTDISTFQLITTSHFLSDLEYYADSRLLKFTF
                     NSILLPDSTTNEPGSHGYIQYSLSPDHTLTIGDSISNYAGIVFDFNDPVYTNFATTNI
                     VCPKFHFYADTDGDGYGDDALDTLACDAPEGFVENNLDCNDGNLLIYPLSSEICNALD
                     DDCDGIADEGLVTYFLYADMDDDGYGDADL"
     gene            complement(3007..3609)
                     /locus_tag="PLM90_11945"
     CDS             complement(3007..3609)
                     /locus_tag="PLM90_11945"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ90137.1"
                     /translation="MIILIIALGIIMLPVVYNVFFPLKKPDLSNYFKPGQTYSSKGEG
                     ITQTIIRQEDNKVYCELRFAPFAPGPPEHMHEQMPETIQVVQGTLSAKINGEVVQIHE
                     GERVNLPAGVFHKIFNQTDKEVVLRPQLPEDHIPVEFAYSLDQLYPLMTSKNLTLKMF
                     AKICVLDELFDSIPVGPPPAVFRFIKKAVKPYARLFGVKP"
     gene            complement(3847..4050)
                     /locus_tag="PLM90_11950"
     CDS             complement(3847..4050)
                     /locus_tag="PLM90_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90138.1"
                     /translation="MVSHDHDGDWQFMCGIQAHTMANAKIITYFEALELDPTLLELEI
                     LPVGHHALRSSKDDAWQIIPIKS"
     gene            complement(4419..4934)
                     /locus_tag="PLM90_11955"
     CDS             complement(4419..4934)
                     /locus_tag="PLM90_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90139.1"
                     /translation="MKNALLLLFIGCLLSSCSGFSNASKHIENTEPEYVIQDTFLITG
                     FYRISNNNEGLKRTQLYDTATYYVIPTPVISIKHFKSIEMAKGYYGHKVLSLQFDKMG
                     TDLWFAETTEAQGEKIAVIVNDTLISAPRVNEAIPVGRASLDLGPENEIKLQQFKSDI
                     EKEMEKYASRK"
     assembly_gap    5447..5546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5764..>7284
                     /locus_tag="PLM90_11960"
     CDS             5764..>7284
                     /locus_tag="PLM90_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HMZ90140.1"
                     /translation="MKLRINTSETFAQRHIGPNSNDVQAMLQAIGADSIDSLVNKTVP
                     ENIRLKRPLNISAPLSEYNYLKHLRNLASKNKVFRTYIGMGYYNTITPSVIRRNIFEN
                     PGWYTQYTPYQAEIAQGRLEALLNFQTMVSDLTGFEIANASLLDEGTAAAEAMIMFFH
                     HKNKKDVTSPKFFVSDLCFPQTIEIIRAKAEPLGIDVVVGDHRNAQIDDTYFAALMQY
                     PAGNGMVFDYTSFIAHLHTHNVSSIVAADILSLAVLQTPAAMGADCAVGNTQRFGVPM
                     GFGGPHAAYFATKDAFKRDIPGRIIGVSVDRDGRPALRMALQTREQHIRREKATSNIC
                     TAQALLAIMAGMYAVYHGADGIRQIAEKVHGLTTVLDNGLKQLGFTQSNAQYFDTVHV
                     ACGSLSGLVHQHAIAKHMNFYYHQNGDVQITLDETTELSDVQDILSVFAEAKGSATVT
                     LPSIDSLQSSLPASSLREDAYLQHPIFNTFHSESEMMRYIKRLENKDLSLTTSMIPL"
BASE COUNT         2019 a         1426 c         1542 g         2197 t
ORIGIN      
        1 cgaggtcagc atcgccatag ccatcatcat ccatatcggc atacaagaaa taggtgacca
       61 atccttcatc tgcaataccg tcgcaatcgt cgtctaaagc attgcagatt tcagaggata
      121 gtggataaat caagaggttc ccgtcattac aatcgagatt attttcaaca aacccttcag
      181 gtgcatcgca tgcaagtgta tctaaagcgt cgtcaccata tccatcgcca tcagtatctg
      241 catagaaatg aaatttaggg catacgatat tggtggtagc aaaatttgta taaacaggat
      301 cattgaaatc aaacacgatg cctgcataat ttgaaatgct gtcgccaatg gttaaagtat
      361 gatcaggact caatgagtat tggatgtatc catgactgcc aggttcgtta gtggtgctgt
      421 cggggagtaa aatgctgttg aaagtgaatt ttagtaagcg tgaatctgcg taatattcta
      481 aatcacttaa aaagtgggat gttgtgatca gttgaaaagt gctgatatca gttccgaccg
      541 gtaaaatatt atttacaatg atattgaaag ctgtatccgt accaatattt tgaaaattga
      601 tggtgtaatt aagatgatcg gtgacagcaa gttcttcagg ggtgagttca accagattca
      661 cgatcatatt attcggatca taggaagcaa ttatttcgtt tacactattt gaaatactat
      721 tgtcaactat gctgtaaaca tccggtgaat aaataagcgc tgaagatgtc accatgtcgc
      781 ccgcatcagc agtttcactg acgataaaag ttactgttat gagtccactt tcgaagggag
      841 caatggttcc taacgaccat gtaatggaat catcgtataa tgtatatcct gccaggtcgg
      901 cactcataaa cgacaacaag ggatcgtata ttaaattgat ttgaccatta atagatgttg
      961 tgcctatgtt ctgataggag agggtatatt ttacagttgc accgggacga aacaaataaa
     1021 ttggagtgat atcgatcacc agagcattca ttacgctgtc gggttgaaat ccaaagttcc
     1081 tcaaggaatc aaccgtataa aaactatcga tcactgttgt ataattggcc ggaacagaag
     1141 tataatacgg cagcgtgtca gaactgtagg agaaggtatc caaacttgtt acttgaaatg
     1201 cataatatcc tgatgaatca ctgaattcag tcaaaccttc gggagaaata tatacgggct
     1261 tgtaggttaa tggtggttcc cctgcgtcat gcagtgtatt ggcattcagg tcggcatata
     1321 aggtgccgga gatggtagaa aaatccggtg taagctttat cagccataaa tcataattgc
     1381 ccaagttggg agctgtctta tttcctgtga ctattgatct ggaagaaccg gataataata
     1441 cgccgccatc attggtgatt gtcatggatg taaggtcatc gtcgtttgta ccaccgaaaa
     1501 ccttttgcca gaggatattt cctgattcat cagttttgac tatccaataa tcgtaatcac
     1561 cataggtgtt tgaatttttg tcataaccta tggatgatgc agatttgcca cccaacatat
     1621 atccgccatc ctgtgcttgg ataatacacc gtaaataatc aaggttatta ccttggattg
     1681 tattttccca taccacttca ccggtagcat taatcttcac cacccagtag tcatagtaat
     1741 aaattgaatt ttcagattta tcgtaccctg cgggcgactg tgattcagcg gcaagtaaat
     1801 atccgccatc cgcagttttg attgcatcca cacaattgtc aaaatgcgtt ccgccgatca
     1861 cattttgcca ttcgatatca ccgatactat taatctttat gatccaaaaa tcggtgacat
     1921 caatcatggg ctcagtttta tcgcctgtga tatcagaatt gctgtaaccg cataataagt
     1981 aaccaccatc atctgattgg atagctctca ctagttgatc gtcccaaaaa gcgtaatatt
     2041 cgcgctgcca ttcaatagtt cctatattat ttaatttgta gacattgtaa ttatatataa
     2101 aactacctgc ctgttccttg tttgcaccta aaatataacc gccatcagga gtttggttga
     2161 tactgccaac aaagtcgtca taaccaccac caaatacttt ctgccattcg atatgtccgg
     2221 tgctattgag cttaaccacc caggcatccg gatttccgta aggacctatc gtattagatg
     2281 tcttaacacc tgatatatcg gaattagaag ttccaccaag tatatagccg ccgtcgtcag
     2341 tgagtgataa atagttcagg tagtcatcgc cggaaccacc gatggttgtt tcccattcga
     2401 cattggcagc ggaatctgtt ttaataatcc aatagtcggc acccccatag ctaggctctg
     2461 atttatatgc agaaatacct gaaagcgagc tggagccaaa aataaatcca tggtccgggg
     2521 tcattaatac gatatctgct gtttcgtaat ctgtgccgcc aattgttttc tgccaatcca
     2581 tggccaatga ctgaccatac aggctgccac ttgtaactag cgcaagaaga aatagggatt
     2641 tgaggtataa agttttcatg gaaatgggag ttgtatgcaa atttagggaa atgtagggaa
     2701 aggagggaat aattagggga tagaaatgta ggagtgtgga agtctgcaag tgggcaggtt
     2761 tgcaagtgtg caggtttgca agtgaaatac agatgtgttc atttgcttgt gtgttctggt
     2821 tggaaattta agtacgttgc tgaaggtatt tcgccccctt actctccgaa cttactatga
     2881 agaatacttg tggatggctt cgtaatcctt ttgtactttg ccgtcgataa aacgattttg
     2941 tatatttatt gatgttcttt taagggagac atggttgaaa gtaaataaat ataattctcg
     3001 aaagtgctat ggttttacgc caaataaccg agcgtacggc ttcacagctt ttttaataaa
     3061 tcggaatact gctggtggtg gacctaccgg tattgaatca aataattcat ctagcacgca
     3121 tattttagca aacatcttca aagtaaggtt tttgcttgtc atcagtggat acaattgatc
     3181 caatgaataa gcaaattcaa cgggtatatg atcttccggg agctgtgggc ggaggactac
     3241 ttccttgtca gtctgattga atatcttatg gaaaacgccg gcagggagat ttacacgttc
     3301 accttcgtgt atttgcacaa cttcgccatt aattttcgca cttaaagttc cttgtaccac
     3361 ttgaatggtc tcaggcattt gttcatgcat atgttccggc ggacccggtg caaaaggcgc
     3421 aaatcttaac tcgcaatata ctttattatc ctcctggcgt atgatcgttt gtgttatccc
     3481 ttctccctta gacgaatatg tttgtccggg tttaaagtag ttagaaagat cgggtttttt
     3541 taaaggaaaa aatacattat aaacgaccgg gagcattatt atgcccaagg caatgattaa
     3601 aattatcata atgttatgag ttttgttggc ttgtcaaaaa taatcaataa gatttcctat
     3661 ctgctaataa attattttat acctgtagcg taaacattat ctgctctagt gcctcgcgtc
     3721 gaatatggac catcgtagat aattgaaatt ggtaagcaat acccttttcc gattacatcc
     3781 aaacagctcc ttataaacac ttccccgatc acgtagattt tttgatcaaa tatggctatg
     3841 tatgaattaa cttttaatcg gaataatttg ccatgcatca tctttcgatg accgcaacgc
     3901 atgatgaccg acaggaagga tttccaattc gagaagtgtg gggtctaatt ctaatgcctc
     3961 aaaatatgtt attattttag cattagccat tgtatgcgct tgtattccac acataaattg
     4021 ccagtcgcca tcatggtcgt gggataccaa aagtataggg agttccctat tcaatacatg
     4081 atcgcaagta aaacacgcgg tttgcgggga ctcgggttca aatataattt ccatgttatg
     4141 gtaaaggtct ttaaattagc ataagcttgc aagtggaatg cagttgtgtt catgtgctat
     4201 tgagttcggg tgtttttgtg cgaaatgcta cgtgttcatg tggttgtgtg tacaggctct
     4261 ttgcagccga aaataaccag tacccaaact gtataccaga atgccgaaaa tgcgtatatt
     4321 ggttttgtca ccgggggctt tttaaatagt ttttttgggg aagtaaatac cgattaaaat
     4381 agttagtttg tgcatacaaa ttgaccccaa gattaaattt attttcgaga cgcgtatttc
     4441 tccatttctt tttctatgtc ggatttaaac tgctgtaatt taatttcatt ttcaggacct
     4501 aaatccagcg atgctctgcc aaccggaatt gcttcattta ctcgtggggc agaaattaag
     4561 gtgtcattta ctattacagc aattttttca ccttgggctt ctgttgtttc ggcaaaccat
     4621 aggtctgttc ccattttgtc gaattggagt gacaatacct tatgaccata atacccttta
     4681 gccatttcta ttgacttaaa gtgctttatt gaaataacgg gagtgggaat aacatagtat
     4741 gtggcagtat catataattg tgtgcgcttt aggccttcat tattgttgct tattctgtaa
     4801 aatcctgtga tcaggaaggt atcttgaatg acatattccg gttcggtgtt ttcgatatgt
     4861 tttgatgcat tgctaaagcc actgcaggaa gataataagc atccaatgaa taacaggaga
     4921 agggcatttt tcatgagatg aaaatagctg taatgtttta tagataaaag gatatttgga
     4981 tttttgtata aacatgcgac cccttcgggg tcgattgatg ttgtgtgtgg ttctggggta
     5041 taaatgtgcg acccctacgg ggtcgattga tgttgtgtgt ggttctgggg tataaatgtg
     5101 tgacccctac ggggtcgatt gatgtttcgc ggtaattttt tttgatttga ttgaacctaa
     5161 aaggttcaaa agaatttgca acaatctcat tttcttgcta cccctcaagc ttactgccta
     5221 tcaatcaata aagaaccacc tcccccaaat cccgaaggga ttgcctgtta atacattccc
     5281 catgcatgta accccccgac cccgaagggg tcgcatattt atggccccgg tggattgaaa
     5341 tagcattgtg tatctgagat catcgacagc gcatgccatg atgccacttc atcttcattt
     5401 tattgcgtta ggatgcatgc ttttctagcc ccgattgcag cgaaaannnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnttgc agcgaaaagc gggtgaggag ctccgggcgg
     5581 actatgaatg ttgtgctcct gaatagagcg tttatttcat aaagtgttgc agagattgcg
     5641 cataatcggt tagttggctg attggctgat tgatttatgt gaacatcctg tttacagtgt
     5701 tgttctttgg cctaaagcct tgtctataaa tcgtaacttt gtgccctaat tcaattgttg
     5761 ccagtgaaac ttcgcataaa cacctccgaa acctttgccc agcgccatat tgggccaaat
     5821 tctaatgacg tgcaggctat gttgcaggct attggggctg attctatcga cagccttgtg
     5881 aataagaccg taccggaaaa tattcgcctg aagcgcccgc tcaatatcag cgcgcctttg
     5941 agcgaatata actacctcaa acatctgcgt aatctggctt cgaaaaataa ggtttttcgc
     6001 acctatatcg gcatgggtta ttataatacc ataacaccca gtgtaatccg ccgcaatatt
     6061 tttgaaaatc cgggttggta tacgcaatat acgccgtatc aggccgaaat tgctcaaggt
     6121 cgcctcgagg cgctgctgaa cttccagact atggtgagcg accttaccgg tttcgagata
     6181 gctaatgcga gtctgctgga tgaaggaacg gccgctgctg aagcgatgat catgttcttc
     6241 catcataaga acaaaaagga tgtaacatca cccaagttct ttgtgagcga cctttgcttc
     6301 ccgcaaacaa ttgagatcat ccgggcaaaa gccgaaccgc ttggtataga tgtagttgtg
     6361 ggtgatcaca gaaatgcgca aatcgacgat acctacttcg ctgccttgat gcagtatcct
     6421 gccggaaatg gtatggtgtt cgattatact tcattcatcg cccatttaca tacgcacaac
     6481 gtttcttcaa ttgttgccgc tgatatattg tcgttggcag tattgcaaac gcctgctgcc
     6541 atgggtgccg attgtgctgt tggcaataca caacgctttg gtgtaccgat gggatttggt
     6601 ggtccgcatg ccgcatattt tgctactaaa gatgccttca aacgcgatat tcccggtcgt
     6661 atcatcggtg tgagcgttga tcgcgatggt cgccccgcat tgcgcatggc attgcagaca
     6721 cgtgagcaac atatccgtcg cgaaaaagcg acctctaata tttgtacggc tcaagccttg
     6781 ctggctatta tggccggtat gtatgctgtt tatcatggtg cagatggcat cagacaaatt
     6841 gcggagaaag tgcatggact tacaactgtg ttggataacg ggttgaagca acttggcttt
     6901 acacaatcaa atgcacaata ttttgacacc gttcatgttg cctgtggttc attgagtggc
     6961 ctcgtgcatc agcatgccat tgcaaagcac atgaatttct actatcatca aaacggcgat
     7021 gttcaaataa cactcgatga aactaccgaa ttgagcgacg tgcaagatat tttatctgta
     7081 tttgcagagg ctaagggaag tgcaactgtt acattgccat cgatcgatag tttgcaatca
     7141 tcgttgccgg cttcttcatt acgcgaagat gcttatttac aacatccgat ttttaatact
     7201 tttcacagtg agagtgaaat gatgcgttat attaagcgtt tagaaaataa agatctttca
     7261 ttaacaactt cgatgatccc ttta
//
LOCUS       DAOQOU010000162         7166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5883_length_7166_cov_6.804106, whole genome shotgun sequence.
ACCESSION   DAOQOU010000162 DAOQOU010000000
VERSION     DAOQOU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5883_length_7166_cov_6.804106"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLM90_11965"
     CDS             <1..607
                     /locus_tag="PLM90_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ90141.1"
                     /translation="QYEEASVAGNIHEVKTFTEKPDQELARTFIKSGDFLWNSGIFIW
                     SVKSIMSAFDLHQQDIHQAFWAARKAFGTKDEKWMVEKAYSICPNISIDYAIMEKADN
                     VYVIPASFGWSDLGTWASLWDNYDKDYYGNAVKGKKVMIYDATNNMIMSDDDKLVVVQ
                     GLEDYIIVDTKDVLIICRKDKEQEIKDITSDLKRKEMDDFL"
     gene            complement(728..1816)
                     /locus_tag="PLM90_11970"
     CDS             complement(728..1816)
                     /locus_tag="PLM90_11970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ90142.1"
                     /translation="MATICFFNSIRFWGGGEKLHLELAVGCKALGHHVVIACNKNGIL
                     WQRAQEAGLECVDVRVTKFSFLNTFHINKISNTLASIQANAIIFTASQDAKTGGAAAK
                     KAGINKIVYLRGLAARVKNSYFNRRLFTKTLTHIIANSEETKSLLLQKMQHVKGIPNI
                     PVVYHGINIVETQSRNKHPFIAQHGNGIILGNAGRLTAQKAQKDLIDVAIKLRDLQIN
                     FTLFIAGEGEERTSLENAIKLHQLQDQVFLTGFVDDIPAFMQSIDIFVLSSHWEGFGF
                     VIAEAMSHELPVVAYDISSNPEIIADNETGLLVPCGNTDAFALAIKQFAEDQDLCKKY
                     GIAGRKRVIEKFEFSTQLKQLLAVIEIN"
     gene            1918..3075
                     /locus_tag="PLM90_11975"
     CDS             1918..3075
                     /locus_tag="PLM90_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HMZ90143.1"
                     /translation="MADHTVQIKSKLPNTGISIFARMTALANQHGAINLAQGFPDFMS
                     SEKLISLVHAYMQKGMNQYAPMPGLLALRERIAEKIKFIYHNTYDPASEVTITAGATQ
                     AIYTSIAATIRDGDEVIIFEPAYDSYAPAIRVNGGIPVPVELKAPDYKIDWNIVKKLV
                     SQRTRMIMINTPHNPTGTVLDQSDIKQLIRIVHGKDIIVLSDEVYEHIIFDGIRHESV
                     LHYPELRERSMVIFSFGKTYHNTGWKIGYVLAPEYLTKEFRTVHQFEVFSVNTPIQYA
                     LSDFMEDRSEYEKVSNFYAAKRNLFSDMLKGSKFVLKPSAGTYFQLLDYSAISDLGDQ
                     EFADLLTIEHKVAAIPLSPFYRNGSDAKMLRFCFAKQDSTLQKAADILCKI"
     gene            3063..3860
                     /locus_tag="PLM90_11980"
     CDS             3063..3860
                     /locus_tag="PLM90_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ90144.1"
                     /translation="MQDLKVTLVQVDLAWENKQANLDYISTLLKDVRDTDLIILPEMF
                     STGFSMRSKDLAENMMGNSVRWMRDLASAKNAAVCGSLIIEDDEKFYNRLIWMEPDGT
                     SAHYDKRHLFSLAGEDKHYTGGKHKIFPELKEWKILPLICYDLRFPVWSRQSPPFTEL
                     GANPYHLLVYVANWPERRVYAWEQLLIARAIENQCYVVGVNRIGMDGNDVYHNGCSLV
                     VDPMGQKLYESKGEESVKTLTLSGLQLESIREKLPFLDDRDEFELRR"
     gene            3955..4809
                     /locus_tag="PLM90_11985"
     CDS             3955..4809
                     /locus_tag="PLM90_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ90145.1"
                     /translation="MITDKIVKPSSGFFMFFVFLLMSIGIGYAMMNVHPAYSLAFIPL
                     FFVLIGFFIINPNDAKVLVLFGKYAGTVTENGFYWANPFYGKHRVSLKARTLDGDKIK
                     VNDKLGNPIIIGAVVVWQVDDAAKAKFEVENYEMYVKLQSDTAVRHLAGSFSYDHFSD
                     NDSEEITLRSGGEHINEMLVKELKDRLQLAGVKVIEARITHLAYAEEIAGAMLQRQQA
                     TAVVAARTKIVEGAVGMVQLALQKLSKEQIVELDEEKKAAMVSNLLVVLCSDKTANPV
                     VNTGTLYH"
     gene            4819..5322
                     /locus_tag="PLM90_11990"
     CDS             4819..5322
                     /locus_tag="PLM90_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90146.1"
                     /translation="MEFQEEQRISVMWMRILFLIIIIGSLAGVLLGGMNSGHEPQISD
                     YLGAVFCVAIVSLVYYMVFYTKLKTSVSMMGFAYEYFPFVWREKTITKEMIKSWQMQT
                     MKSGFQYGGYGIKQSYFPKKTAFMMGSRNAVEFKLHDGKTLIFTTNNPEQLQLALRKY
                     FSQTEIR"
     gene            5322..5510
                     /locus_tag="PLM90_11995"
     CDS             5322..5510
                     /locus_tag="PLM90_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HMZ90147.1"
                     /translation="MAKEDNKSAKDRQAFILRVSPELLKAVEKWAADDFRSVNGQIEF
                     LLHEALKKSGRMKKRIEE"
     gene            complement(5514..5687)
                     /locus_tag="PLM90_12000"
     CDS             complement(5514..5687)
                     /locus_tag="PLM90_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90148.1"
                     /translation="MVDKFQQWRFTSYHLAISSEHNTIQSNVLPLFGGKAKCIDFHKA
                     FIQSREAERVFTE"
     gene            5741..6496
                     /locus_tag="PLM90_12005"
     CDS             5741..6496
                     /locus_tag="PLM90_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90149.1"
                     /translation="MGRSGDVVNFSGYIWDVKIAESYQVGPGPNYFSGYYEDVFVDEQ
                     GYLHMRIAEHDGKWFSSEVVSQMNMGYGKYSWVVQGDLENIPENITLGLFTWDNNTFF
                     DQANSEVDIEFSKWGVASEENTMLYSVQPVNFGPTYPERTHLIETEVGDLVGVTTHTF
                     IWTDTLITWNSYYGDTESEDKRIGTWYFNLDNPPRVKNENGNSSAAIVIPTPGETTNA
                     RINFWLLPWISSSPTDGLEREMVIRKFSYEAAE"
     gene            complement(6516..>7166)
                     /locus_tag="PLM90_12010"
     CDS             complement(6516..>7166)
                     /locus_tag="PLM90_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ90150.1"
                     /translation="YPVLYMLDGQNLFDDQLSYAGEWGIDESMDSICHLGETTAIIVG
                     IDHAGDARLAEYTPWKVARFNESGKGDLFAQFVVEELKPHIDSLYRTLPDRIHTGIGG
                     SSVGGVMSQYLVLQYNDVFGNALVFSPSFWISKQNFQLAEAYTSDKPTAIYYLAGGRE
                     GDDDDMQENAIAMYRLLQKKQINNLTLRIVIEGNGIHTESFWRKGFSAAYLWLFEK"
BASE COUNT         2131 a         1279 c         1622 g         2134 t
ORIGIN      
        1 ccagtatgaa gaggcttcgg tagccggaaa catccacgag gtaaagactt tcacagaaaa
       61 accggatcag gaattggcca gaacctttat taaaagtggc gatttcttat ggaattcggg
      121 gattttcatc tggagcgtga agagcatcat gagtgctttc gacctccatc aacaggacat
      181 ccaccaggca ttttgggccg cccgtaaggc tttcggcact aaggatgaga aatggatggt
      241 tgaaaaagct tacagcattt gcccgaatat ttctatcgac tacgccatca tggaaaaggc
      301 tgataatgtg tatgtaattc cggcatcttt cggatggagc gacctcggaa catgggcgag
      361 cctgtgggat aactacgata aagactacta tggcaatgcc gttaaaggca aaaaggtgat
      421 gatatacgat gctaccaata atatgattat gtctgatgat gacaagttgg tggttgtgca
      481 gggacttgag gattatatta ttgttgacac caaagatgta ctcatcatct gcagaaaaga
      541 taaagagcag gagatcaaag acattacttc tgatttgaag cggaaggaaa tggatgattt
      601 tctttgagtg cgaacacggc tgtcagatct ataatattta aataatatca ttaccttctc
      661 gggaagcgaa tattattttg aggcatgtga agtaacgcaa aatcaggaaa gtgttttgca
      721 gtcggcatca attaatttca ataactgcta ataactgctt taattgtgta gagaattcaa
      781 atttttctat tacacgtttg cgtcctgcaa tcccgtattt tttgcataaa tcttgatctt
      841 ctgcgaattg ttttattgct aatgcaaagg catcggtgtt accacatggg acaagcaaac
      901 ctgtttcgtt atcagcaatg atttcaggat tactgctgat atcgtaagct actacgggaa
      961 gctcatgcga cattgcttct gcgatcacaa atccaaaacc ttcccaatgc gaggatagca
     1021 caaagatgtc gatactttgc atgaaggcag ggatgtcatc aacaaaaccc gtcagaaaaa
     1081 cttgatcctg taattgatgc agcttgatgg cattttcaag tgatgtgcgt tcttctcctt
     1141 ctcctgcgat aaagagcgta aaatttatct gtagatctct gagtttaatg gctacatcaa
     1201 tgagatcttt ttgcgctttt tgtgcagtta gtcggccggc gttacccaga attattccgt
     1261 tgccatgttg cgcaataaat gggtgtttat ttcgcgattg agtttctaca atattaatac
     1321 catgataaac aacaggtatg tttggaattc cttttacatg ttgcattttt tgaagaagca
     1381 gcgattttgt ttcttcagag tttgctataa tatgcgttaa tgtttttgtg aataggcgtc
     1441 tgttaaagta gctgttttta actcgtgcag ctaatccgcg cagataaaca attttgttta
     1501 ttccggcttt ctttgcagca gcgcctcctg ttttagcatc ttgcgaagca gtaaaaatga
     1561 tagcatttgc ctgaatgctt gcaagcgtat ttgagatttt atttatgtgg aatgtattta
     1621 aaaaggagaa tttagttaca cgtacatcca cgcattccaa cccggcctct tgcgcgcgtt
     1681 gccacaaaat accattttta ttacaggcaa ttacaacatg atgtccaagc gctttgcatc
     1741 caacagcaag ttcgagatgt aacttttccc cgccacccca aaagcgtatt gaattaaaga
     1801 agcaaattgt agccacgagg taaaaataac ggaattcaag gatttggtac cgtatgaatt
     1861 gaatcaaggg gtttggatgc tatttattga tacatttatt tccattactt ttatcccatg
     1921 gccgatcata cagtacagat caagagtaaa ttgcccaata ccggaatttc catttttgcc
     1981 cggatgacag cattggcaaa tcagcatggg gcgatcaatc tggcgcaggg cttccccgat
     2041 tttatgtcga gtgaaaaact tatttccctg gtacatgcat atatgcagaa gggaatgaac
     2101 caatatgcgc caatgcccgg tttgcttgca ttgagggaaa gaattgcgga gaaaattaaa
     2161 tttatatatc acaatactta tgatccggct tcggaagtta ccatcacggc aggagcgacg
     2221 caagcaattt atacaagtat agcggcaacc attcgcgacg gtgatgaagt aatcattttt
     2281 gaaccggctt atgatagtta tgctcctgca attcgtgtaa atggcggtat tcccgttccg
     2341 gtggaattaa aagcacctga ttataaaatt gactggaata ttgtaaaaaa acttgtctcg
     2401 caacgcacgc gaatgatcat gattaataca cctcataatc caacgggtac agtcttggat
     2461 caaagtgata ttaagcagtt aattcgtatt gtgcacggaa aagatattat tgtattaagt
     2521 gatgaggtat atgagcatat tattttcgat ggcatcaggc atgaaagtgt tttacattat
     2581 ccggagttga gagaacgaag tatggtcata tttagtttcg gaaaaacata tcacaatacc
     2641 ggttggaaaa taggatatgt attagcccct gaatatttaa caaaggagtt cagaacggtt
     2701 catcagtttg aggtgttttc agtgaataca cccattcaat atgcgctttc cgactttatg
     2761 gaagatcgca gtgagtatga aaaagtcagt aacttttatg ctgcaaaacg caatttattt
     2821 agcgatatgt taaagggttc gaaattcgtt ttaaaaccgt cagcgggaac atattttcag
     2881 ttgttagatt atagtgcaat ttctgatctg ggtgatcagg aatttgctga tctgctgact
     2941 atagaacata aggttgctgc aattccacta tctccttttt atagaaatgg gagtgatgca
     3001 aaaatgttga gattttgttt cgctaaacaa gacagcacac ttcaaaaagc tgcagatata
     3061 ttatgcaaga tctgaaagtc acacttgttc aagtagacct ggcctgggaa aataaacagg
     3121 caaaccttga ttatatttct acattgctaa aagatgttcg ggacacagac ctaatcatat
     3181 tacctgaaat gttcagcaca ggattttcca tgcgatcgaa agatctggct gaaaatatga
     3241 tgggtaattc tgtgaggtgg atgagggatt tggcaagtgc gaaaaatgct gcagtgtgcg
     3301 gaagtttgat tattgaggac gatgagaaat tctacaatcg tttgatttgg atggagcccg
     3361 atggcacgag tgcgcattat gacaagcgac acttgttttc attggcaggt gaagacaagc
     3421 attataccgg tggaaagcat aagatattcc ctgaattgaa agaatggaag atattgccat
     3481 tgatatgtta tgatttaagg ttcccggtgt ggagcagaca atctccaccg tttaccgagt
     3541 tgggagccaa tccttatcac ctgctggtgt atgttgccaa ttggccggag cgaagggttt
     3601 atgcgtggga gcagttgctg atagcccgtg caattgaaaa ccagtgttat gttgtaggcg
     3661 tcaaccggat cggtatggat ggcaatgacg tttaccataa cggctgttcc ctggtagtag
     3721 accctatggg gcagaaacta tacgagtcga aaggcgaaga atccgttaaa acacttacac
     3781 tgagcgggtt acagctcgaa tcgatacgag aaaaacttcc atttctagat gatagggatg
     3841 agtttgagct aaggcgttaa tgttaaattt tcacaaaatc atacaatttg ttgggatttc
     3901 tgtcgtcatc actgatatct ttgtgatatc attttgaaat ctaaaactaa aactatgata
     3961 accgacaaaa ttgtgaaacc gagttccggc ttctttatgt tttttgtatt cctgttaatg
     4021 agtataggca ttgggtatgc gatgatgaat gtgcatccgg cttatagtct tgcttttatt
     4081 cctttgtttt tcgtgttgat tggctttttc attatcaatc cgaatgatgc caaggtgctt
     4141 gtattatttg gaaagtatgc aggcacggta acggagaacg gattttattg ggcgaatcct
     4201 ttttatggca agcatcgtgt atcgcttaag gcgaggactt tggatggcga caagatcaaa
     4261 gtaaacgaca agctaggtaa tcctatcatt atcggagcag ttgttgtttg gcaagttgat
     4321 gatgctgcga aagcaaaatt tgaggtagag aattatgaaa tgtatgtaaa gctgcaaagt
     4381 gataccgctg ttcgtcactt ggcgggttca ttttcttatg atcattttag tgataatgat
     4441 agtgaggaga ttaccttgcg cagtggaggt gagcacatca acgaaatgtt ggtgaaggaa
     4501 ttaaaagatc gtttgcaatt ggcaggtgtg aaagtgattg aagcgagaat cacacatttg
     4561 gcctatgccg aagaaattgc cggtgcgatg ttgcaacgcc agcaagcaac tgcagttgtt
     4621 gcggcaagaa ctaaaattgt ggaaggagct gtaggcatgg ttcaattagc attgcagaaa
     4681 ttatcgaagg agcagattgt tgagcttgac gaagaaaaga aagcggcaat ggtgagtaat
     4741 ttattggtgg tgttgtgttc tgataaaaca gcgaatcctg ttgtaaacac cggtacatta
     4801 tatcattaat tcagtgttat ggaatttcag gaagagcaac gaatcagtgt aatgtggatg
     4861 cgcatattat ttctgattat aataatcgga agtctggccg gcgttttact tggcggcatg
     4921 aacagtggcc atgagcccca gatttccgat tacctgggag ctgtattttg cgttgcaata
     4981 gtttcgttag tgtattacat ggttttttat actaaactga aaacatctgt ttctatgatg
     5041 ggttttgcat atgaatattt tccatttgta tggcgagaga aaactattac gaaggaaatg
     5101 atcaagagtt ggcaaatgca gacaatgaag agtggatttc agtatggggg atatggtatc
     5161 aagcagagtt atttccctaa aaaaaccgca tttatgatgg gatctcgtaa tgcagtagag
     5221 tttaagttgc atgatgggaa aacattaatt tttactacga ataatccgga gcagttgcaa
     5281 ttggcgttgc gaaaatattt ttcacagaca gaaattcgtt gatggcaaaa gaagacaata
     5341 aatcggcaaa agatcgtcag gcctttatat tgcgggtgtc gcctgaatta ctgaaggctg
     5401 tcgagaaatg ggctgccgat gatttcagga gtgtgaatgg tcagattgaa tttttattgc
     5461 atgaggcatt aaagaagtcg ggacgcatga agaagcgtat tgaagaataa attttattct
     5521 gtgaatacgc gttctgcctc gcgagattga ataaacgcct tatgaaagtc aatgcatttg
     5581 gctttgcctc cgaacagcgg cagcacgttc gattgaatag tattatgttc gctgctgata
     5641 gcgaggtgat atgaagtgaa tcgccattgt tgaaatttat ctaccatgcc atgatgttga
     5701 gcgttgatat ggatgtaagc agtcagatcc tgaagatgta ttgggaagaa gtggtgatgt
     5761 cgtaaatttc tcaggatata tctgggatgt aaaaattgcc gaatcatacc aggtagggcc
     5821 cggtccaaat tatttttcag gatattatga agatgttttt gtcgatgagc aaggatattt
     5881 acatatgcgc attgccgaac acgatggcaa atggttttcc agtgaggtgg tatcgcaaat
     5941 gaacatgggt tatggaaaat attcatgggt tgttcaaggc gatcttgaaa atattcctga
     6001 aaatatcacg ctgggtttat ttacctggga taacaatact ttttttgatc aggcgaacag
     6061 cgaagtggat atcgaatttt caaaatgggg cgtggcatct gaagaaaata ccatgttata
     6121 tagtgtgcaa ccggtgaatt tcggaccaac ctatcctgaa cgcacacatt taatagaaac
     6181 tgaagttggc gatttggtgg gtgttaccac acatactttt atttggaccg atacacttat
     6241 cacctggaat agttattatg gcgatactga atctgaagat aagcgtatcg gcacctggta
     6301 ttttaatttg gataatccgc caagagtaaa aaatgaaaac ggaaacagct ctgcggctat
     6361 cgttattcct acgcccggag aaacaaccaa tgcgcggatt aatttctggt tattgccatg
     6421 gatttcatcg tcgccaactg atggactcga acgtgaaatg gtgatcagaa aattcagcta
     6481 cgaggctgct gaataatgat aattaatggc agcacttatt tttcgaacaa ccataaataa
     6541 gctgctgaaa accccttcct ccaaaaggat tctgtatgta tgccattgcc ttctatcact
     6601 atgcgcaacg tcaaattatt aatttgcttt ttttgtaata gcctgtacat cgctatcgca
     6661 ttttcttgca tgtcatcatc atcaccctcc ctgccacctg ccaggtaata aatagctgtt
     6721 ggcttgtctg acgtataagc ctcagctaat tgaaaatttt gcttactgat ccaaaaggat
     6781 ggtgagaaaa ccaatgcatt tccaaataca tcattatact gcaacaccag atactgactc
     6841 ataaccccgc caacactgct accacctatt cctgtatgta ttctgtcggg gagcgtgcga
     6901 tagaggctat cgatatgcgg tttcaactcc tcaacaacaa attgtgcgaa caaatcaccc
     6961 tttccacttt cattaaatcg cgctaccttc caaggtgtat actctgcaag cctcgcatct
     7021 cccgcgtgat cgatgccgac aataattgca gttgtctcgc cgagatgaca aatagaatcc
     7081 atcgattcat caatgcccca ttctcctgca tatgataatt gatcatcaaa taaattctga
     7141 ccatccaaca tatacaatac gggata
//
LOCUS       DAOQOU010000163         7166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5884_length_7166_cov_5.353396, whole genome shotgun sequence.
ACCESSION   DAOQOU010000163 DAOQOU010000000
VERSION     DAOQOU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5884_length_7166_cov_5.353396"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1145)
                     /locus_tag="PLM90_12015"
     CDS             complement(78..1145)
                     /locus_tag="PLM90_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90151.1"
                     /translation="MAALIWVGCSTGKAGSTAQVSVPNNDSLLRYPAEKHLKNIHQLT
                     YGGDNAEGYWSFNSEMLVFQRRNMNEGVPCDQIFYGKVPTDGSRFEYSLLSTGKGRTT
                     CSYFMPGNKSVIFASTHATVDSCIADPDKSHGYLWGVYNSYEIFEADLKGNIIRQLTN
                     NNYYDAEAVVSPKGDKILFTSDRSGDLELWTMNIDGSNLKQITNELGYDGGAFFSPDG
                     NSIIFRASRPKTEEEVKVYKDLLAQQFVKPTAMEIFTCNADGTGLRQITQLGGANWAP
                     FFHPSGKKIIFSSNHVTKRVPFNLFMINLDGSGLEQITYDTVFDAFPMFSYDGKKLVF
                     ASNRNSGGTHDTNLFVADWVD"
     gene            1264..2328
                     /locus_tag="PLM90_12020"
     CDS             1264..2328
                     /locus_tag="PLM90_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ90152.1"
                     /translation="MIKGRKWLMIGSATGLLLIAILVIVLKPSVPGFGKKPDQPVSDS
                     LAMIEQLTPEIGNLSTSIHEDPKNPGLYFARANAYFDYGNLKYALADFEKAYRIDSTN
                     PAHVLGFSDCLFFLNNAEGAIAVLEDYRLHDPNDLDVLVTLAVDYYKLPKPQMQKSID
                     LLNEALKLDIQHADAYFYKGMIYKESGDTAKAISNFQTTVEVDPDFYDAYMQLGLLYA
                     ANKNSLALQYLNNALALDSTSREAHYAIAKYYQDIGKMSTAINYYKSLITDNPQDADA
                     IYNLATIYYGIDSVKAAYRYYDLAIKQSPAKAMCYYGKGLCAEELKNIKEAISLYTQA
                     LNLDPDLVLAEEKLKQLNAE"
     gene            2332..4263
                     /gene="thrS"
                     /locus_tag="PLM90_12025"
     CDS             2332..4263
                     /gene="thrS"
                     /locus_tag="PLM90_12025"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMZ90153.1"
                     /translation="MIKITFPDGNVREYAEGVSSYDIAKSISEGLARKVLSAEVNGEV
                     WDLSRPINTDATFKLFTWDDQKGKSTFWHSSAHLMAEALEVLYPGVKFGIGPAIDNGF
                     YYDIDLGGKVLTQEDLPRIEEKMRELSKKNSVYQRRDVSKAEALQYFTEKGDQYKLEL
                     INDLADGTITFYTQGNFVDLCRGPHIPDTGHIKAIKLLNIAGAYWRGDEKNKQLTRIY
                     GITFPKQGELDEYLKMLEEAKKRDHRKLGRELEIYTTDESVGQGLILWMPNGAAIIEQ
                     LEKLAKRKEDQGGYLRVKTPHIAKEDLYITSGHLPYYADSMYPPMELEGTKYYLKAMN
                     CPHHHKIFDAMPRSYRELPMRLAEYGTCYRYEESGALFGLMRVRGLTMNDAHIYCTEE
                     QFESEFKAVNAMYLHYFKVFGIEKYVMRFSTHEPKKLGQKYLDNPEMWKRTEDMVRRV
                     LIDSGIPYVEVPDEAAFYGPKIDIQIFSAIGREFTLATNQVDFGQPLRFNLTYVDRDN
                     QPKVPIVIHRAPLSTHERFIGFLLEHYAGNFPVWLAPVQVAVLPISDKFNEYAEAVVN
                     VLKKHEIRTTLDTRSEKIGRKIRDAEVKKVPCMLIIGEQEMMNGTVSLRKHGEGDKGS
                     MSPEDFAKSISEEVAEVFA"
     gene            4305..4847
                     /gene="infC"
                     /locus_tag="PLM90_12030"
     CDS             4305..4847
                     /gene="infC"
                     /locus_tag="PLM90_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMZ90154.1"
                     /translation="MKRVDERNYYRIGREIRHPRVRVVGENITTGVYTIQEAQRMAEE
                     LGLELVEITPNADPPVCRIVDFQKFIYEKKKKDKELKAKSKKQEMKEIRFTPHTDDHD
                     FEFKSKHAENFLVDGNKVRAYVMFRGRSIVFKEQGEMLLLKFAQRLEEVGVPDQMPKM
                     EGKRMYITFVPKAPGTKKKE"
     gene            4956..5150
                     /gene="rpmI"
                     /locus_tag="PLM90_12035"
     CDS             4956..5150
                     /gene="rpmI"
                     /locus_tag="PLM90_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HMZ90155.1"
                     /translation="MPKMKTNSSAKKRFKVTGSGKIKRAKAFKRHILTKKSHPRKNRL
                     GNAGLVSDADSNNIKRLLAI"
     gene            5165..5509
                     /gene="rplT"
                     /locus_tag="PLM90_12040"
     CDS             5165..5509
                     /gene="rplT"
                     /locus_tag="PLM90_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HMZ90156.1"
                     /translation="MPRSVPKVASRNRRKKVLKLAKGYWGRRKNVLTVAKNAVDKALG
                     YAYVGRKDKKSAYRQIWIARINAAVRPHGLTYSTFMHKLKTKNIDINRKVLADLAMNN
                     PEAFEAIVKQVA"
     assembly_gap    5512..5611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5842..>7166
                     /locus_tag="PLM90_12045"
     CDS             5842..>7166
                     /locus_tag="PLM90_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMZ90157.1"
                     /translation="MASDQPQQETTTAKPRITKKSLRTAFRILAFLKPYRLAFIFGMV
                     FLLLSSFTTMAFPRLMGILIDSANPSETASWMTNTNLIGLLLGGILLVQSIFSFFRVY
                     LFATVSEKAMADVRIHFFDKLLTLDLPFYEQHRVGELTSRSTNDVDQLQDMLSATLPE
                     FVRQIVTIVFGVGFIFFISPQLTLLMISSFPILVLMAVFYGKFIRKLSKQRQDALAAS
                     NVVVEETMQNVATVKTFTNENHESKRYRTFLHRVVETGIKGAINRGGFTTFLIMGLFG
                     GFVLVIWYGTTLLKSGDIQAGDLFSFVLYTGFIGGAVGGIGDLYGRLQKTVGASERIF
                     EIIDNEGELSLDNEPDTAQRLDGKIEFRNITFSYPTRKDVTVLKDISFTINPGDKIAL
                     AGHSGAGKSTIAQLILQLYLPDAGTLLFDEQAASTFERRQLRSNMALVP"
BASE COUNT         2094 a         1492 c         1599 g         1881 t
ORIGIN      
        1 gggtgcggga aataataaag taaaggcgcc atcgaaagaa attttcgatg agttggataa
       61 ggcttatatg ttttatatca atcgacccaa tcagctacaa acaaattggt gtcatgtgtg
      121 ccgccgctgt ttctgttgct tgcgaataca agtttttttc catcgtagct aaacatcggg
      181 aaggcatcaa acacggtatc gtaagtgatt tgctctagtc cggagccatc gaggttgatc
      241 ataaataaat taaaaggcac gcgtttggta acatggttgc tgctgaaaat aatttttttg
      301 cctgatggat gaaagaaagg tgcccagttt gctccgccaa gttgcgtaat ctgacgcaag
      361 cctgttccat cggcattgca tgtgaatatt tccatcgccg taggcttaac aaactgctgt
      421 gcaagcaggt ctttgtatac tttaacttct tcttcagttt tcgggcgact tgctctgaaa
      481 ataatgctat tcccgtctgg tgaaaagaat gcgccgccgt cgtatcccaa ttcatttgtt
      541 atttgtttga gattggagcc atcaatattc atggtccata attcaaggtc gccactgcgg
      601 tcgctggtaa acaatatttt atcgcctttt ggacttacaa ccgcttcggc atcataataa
      661 ttattgttcg tgagctgacg aatgatattc cctttcaggt cggcttcaaa tatttcgtag
      721 ctattataaa cgccccagag gtagccgtga cttttatccg gatcggcaat gcaggaatct
      781 acagtggcat gcgtagatgc aaagatcacg cttttgtttc ccggcataaa atagctgcat
      841 gttgtgcggc cttttcccgt gctgagcaaa ctgtattcga atcgtgaacc atcggtagga
      901 accttgccat aaaagatttg atcgcaagga acaccctcat tcatgtttct gcgttgaaac
      961 actaacattt cgctgttaaa actccaatat ccttccgcgt tatcgcctcc gtatgtaagc
     1021 tgatggatgt tttttaagtg cttttcagcc ggataacgaa gtagtgaatc attgttaggc
     1081 acgcttacct gtgccgtaga tcctgcctta cctgtgctgc aacctaccca aatgagggca
     1141 gccatgaaaa ttcctgttaa aaatagagcg agttgtttca ttatgcgctg aaaattgcgg
     1201 caaagttacg atgtagacat catttcccct gatataacaa cgttaatacc gtaattttgc
     1261 gatatgatta aaggtcgaaa gtggttaatg attggaagtg cgacaggttt actcctgatc
     1321 gcaattttgg tcattgtcct aaaaccatct gttcccggtt ttggaaaaaa gcctgatcag
     1381 cctgtcagtg attctttggc aatgatcgag caattgacac cggagatcgg aaatctttcg
     1441 accagtattc acgaagaccc taaaaatccg gggctgtatt tcgctcgtgc aaatgcttac
     1501 tttgattatg gcaatttgaa atatgctctt gccgattttg aaaaagctta tcgcatagat
     1561 tctacaaacc ctgcgcatgt cctgggcttc agcgattgcc tcttcttttt gaacaatgcc
     1621 gaaggagcca ttgccgtgct ggaagattat cgcctccacg acccgaatga tttagatgta
     1681 ctagtcacac ttgcagtcga ttattacaaa cttccaaagc ctcaaatgca aaaatcaatc
     1741 gatttgttga acgaggcgct taagcttgat attcagcatg ccgatgctta tttttataag
     1801 ggtatgattt acaaagaaag tggagatacc gctaaagcga tttccaattt tcagaccact
     1861 gttgaagtag atcccgattt ttatgatgcc tatatgcaac tcggattgct atacgcagct
     1921 aataaaaaca gcctggcgct gcaatacctg aacaatgcgc tggcactgga ttctaccagt
     1981 cgagaagcac attatgccat agcaaaatac tatcaggaca tcggaaaaat gtctactgcc
     2041 atcaattatt ataaatcttt aataactgat aatccgcaag acgccgatgc catttacaac
     2101 ctggcaacca tctactatgg catcgatagc gtgaaagctg cctatcgtta ttatgacctg
     2161 gccataaagc aatcaccggc aaaagccatg tgttattatg gcaagggatt atgtgccgaa
     2221 gaattaaaaa atatcaagga agctatttcc ttatacacgc aggctttaaa tttagacccg
     2281 gatctggtat tagccgaaga gaaactcaaa caattaaatg cagaataatt catgattaag
     2341 atcacctttc ccgatggtaa tgtgcgggag tatgcagaag gggtgtcgag ttacgacatc
     2401 gccaagtcga ttagcgaagg acttgcgcgc aaagtattga gcgcggaagt gaatggagaa
     2461 gtatgggacc tcagtcgccc cataaacaca gatgccacct tcaagctgtt cacgtgggat
     2521 gaccagaaag ggaaatctac attttggcat agcagcgctc acctcatggc tgaggcattg
     2581 gaagtgctat atcccggtgt gaagtttgga attggtcctg ccattgataa tggattttat
     2641 tacgatatcg atctgggcgg aaaagtgctt acgcaggaag acttgcctcg cattgaagaa
     2701 aagatgcgcg agttatcgaa aaagaattcg gtgtaccagc gtcgcgatgt ttctaaagcc
     2761 gaagcattgc aatatttcac cgaaaaaggc gatcaatata aattagagct gatcaatgat
     2821 ctggccgatg gcactattac cttctacacc caagggaatt ttgtagacct gtgtcgcgga
     2881 ccgcatattc cggataccgg ccatatcaaa gcaatcaagt tgttgaacat tgccggtgct
     2941 tattggcgcg gcgatgaaaa gaataaacag cttacacgca tctatggtat cacttttcct
     3001 aagcaaggtg agctcgatga gtatttaaaa atgctcgaag aagccaaaaa acgcgaccat
     3061 cgaaaactcg gccgtgaatt ggagatttat actaccgacg agtccgtagg tcaaggcctt
     3121 attttgtgga tgccgaacgg tgctgccatt attgaacaat tggaaaaact ggcaaagcgc
     3181 aaagaagatc agggcggcta tttaagggtg aagacaccgc atattgcgaa agaagatttg
     3241 tacatcacca gtggtcactt gccatattat gcagatagca tgtatccgcc aatggaattg
     3301 gaaggaacaa aatactatct caaggcaatg aattgtccgc atcaccataa gatatttgat
     3361 gccatgccac gcagttatcg cgagttgccg atgcgactgg cagaatacgg cacttgctac
     3421 cgctatgaag aaagtggcgc actttttgga ttgatgcgcg tgcgaggact aaccatgaat
     3481 gatgcgcata tctattgcac agaagagcag tttgaaagcg agttcaaggc ggtaaacgct
     3541 atgtatttgc attacttcaa agtgtttggc attgaaaaat atgtgatgcg tttttcgaca
     3601 catgaaccta aaaaactggg gcagaaatac ctcgacaatc ccgaaatgtg gaaacgcacc
     3661 gaggatatgg ttcgcagggt attgatcgac agcggtattc cgtatgtgga agttccggat
     3721 gaagcagcgt tttatggtcc gaaaattgat attcaaatat tctcggcaat aggacgtgaa
     3781 tttacactgg caaccaatca ggtagatttc ggtcagccgt tgcgatttaa tttgacttat
     3841 gttgaccgcg ataatcagcc gaaagtacct atcgtgattc atagggcacc attgagcacc
     3901 cacgagcgat tcataggatt tttgctggag cactatgccg gtaatttccc ggtatggctg
     3961 gcgccggtgc aggtggctgt gttgccgatc agcgacaagt tcaacgagta tgccgaggca
     4021 gtggtaaatg tgttaaaaaa acacgaaatt cgcaccaccc tggacacaag gagcgaaaaa
     4081 attggtcgca agatccgtga tgcggaggtg aaaaaagtgc cttgcatgct gatcatcggc
     4141 gaacaggaaa tgatgaatgg cactgtatcg ctgcgcaagc atggcgaagg agataaaggc
     4201 agcatgagtc ctgaagattt cgcgaagagt atcagtgaag aagtagcaga agtttttgca
     4261 taaattattg ttaaccaagt aattatataa ccaaaattaa aagtttgaag cgagtagacg
     4321 aacgcaatta ttatcggatc gggcgagaga tccgtcaccc tagagtacgt gtagtgggtg
     4381 aaaacattac caccggtgtt tataccattc aggaagctca gcgcatggcc gaggaacttg
     4441 gtttggagct ggtagagatc actccaaatg ccgatcctcc tgtatgccgc attgtcgatt
     4501 ttcagaaatt catctatgaa aagaagaaaa aagacaaaga attaaaggcc aaaagcaaga
     4561 agcaggagat gaaggagatc cgcttcaccc cacataccga cgaccacgat ttcgagttca
     4621 agagcaagca tgccgagaac tttctggttg atgggaacaa ggtaagggcc tatgtaatgt
     4681 tcaggggccg ttccatcgta ttcaaggagc agggagaaat gcttttgtta aagtttgccc
     4741 agcgcctgga agaggttggt gtacctgatc agatgcctaa aatggagggt aaaaggatgt
     4801 atatcacctt tgtacctaag gctcccggaa ccaaaaagaa agaataacta atcttttcac
     4861 ggcaaagaag ttgcatttgg gctttgaagt ttcagcccga actcctatct ttgcgctccc
     4921 gaactttcgg ggaagtaaac aaaacaagtg tcattatgcc gaagatgaag accaacagca
     4981 gcgcgaaaaa gcgcttcaag gtgaccggta gcggaaaaat caagcgcgcg aaagcgttca
     5041 aacgccatat cctcaccaaa aagagtcatc ccaggaaaaa ccgcctcggt aatgccggtc
     5101 tcgtaagtga tgcggattca aacaacatta aacgtttact agccatctaa taaacaagta
     5161 gattatgcca aggtcagtac ccaaagtcgc gtcgcgcaat cgtagaaaaa aagtattaaa
     5221 gcttgccaaa ggctattggg gccgtcgcaa gaacgtatta actgttgcca aaaacgcagt
     5281 tgataaagcc ctcggttatg catatgtagg tcgtaaagac aagaaatctg cttaccgcca
     5341 aatttggatc gcgcgcatca atgctgctgt tcgccctcac ggacttactt acagcacttt
     5401 catgcacaag ctcaaaacaa aaaatatcga tatcaaccgc aaggtattag ctgatttagc
     5461 tatgaacaat cctgaagcgt ttgaagcaat cgtaaaacag gtagcataag cnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggatcggca ttttcattga tgcaaaggcc
     5641 cgctgaagtg tgttgaatga atacatgcat caaccctgac tcaggcaatt cgcctactgc
     5701 atgcagaata tgctcgtcga tgaggtggaa gccgcgtgta aaagccggca gtatgatgtt
     5761 aaattgctgc accatcaatc aaaaataaaa tgaaaacggt aactactcgt caactatcgg
     5821 ggaatatctt tacaccttct tatggcatct gatcaaccgc aacaggaaac gacaacagca
     5881 aaacccagga tcaccaaaaa aagtctccgc acagccttca gaattcttgc tttcctgaag
     5941 ccttatcgcc ttgcctttat ttttggaatg gtgtttttat tgctctcgag ttttaccacc
     6001 atggcatttc cacgcttgat gggaatactc atcgactcag caaatccaag cgagactgcg
     6061 tcctggatga caaacaccaa tttaattggc ctgttgctgg gaggcatact cctcgttcaa
     6121 tcgatatttt cattcttcag agtatacctt ttcgccactg tttctgaaaa agcaatggcc
     6181 gatgtgcgca tacatttttt cgataaactg cttacccttg atctcccttt ttacgagcaa
     6241 caccgggttg gcgaactaac cagcaggtca acaaatgatg tggatcaatt gcaggatatg
     6301 ttgagtgcta ccttacccga atttgttcga caaatagtaa cgatagtgtt tggtgtggga
     6361 tttatctttt tcatctcacc gcaacttaca ctgctcatga tatcatcatt tccaattttg
     6421 gttttgatgg cagtgttcta cggaaaattc atccgcaaac tttccaaaca aaggcaggat
     6481 gctttggcag cttcaaatgt ggttgtggaa gaaaccatgc agaatgttgc tacggtaaaa
     6541 accttcacca atgaaaatca tgaatcaaaa cgctatcgca catttttaca tcgcgtagtg
     6601 gaaacaggta ttaaaggcgc tattaatcgc ggcggattta ccactttcct catcatggga
     6661 ttatttggcg gatttgtatt ggtaatctgg tatggcacca cattgttaaa atcaggtgat
     6721 attcaagccg gcgatttatt ttcatttgta ctttacaccg gtttcattgg aggtgctgtt
     6781 ggcggcattg gcgatttgta tggcagatta caaaagactg tcggcgcatc cgaacgcatt
     6841 ttcgagatca ttgataatga aggtgaatta tctctcgata atgaaccgga taccgctcaa
     6901 cgtcttgatg gtaaaattga attcagaaat attacgtttt catatcctac gcgaaaagat
     6961 gtaaccgtat taaaagatat ttcattcacc attaatccag gcgataaaat tgcattggca
     7021 ggtcacagtg gcgccggaaa atctaccatc gcgcaactaa tattacaact atatcttccc
     7081 gatgcaggaa cgctgttatt tgatgaacag gctgcatcaa cattcgagcg ccgacaattg
     7141 cgcagcaata tggctttggt gccaca
//
LOCUS       DAOQOU010000164         7125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5932_length_7125_cov_5.078925, whole genome shotgun sequence.
ACCESSION   DAOQOU010000164 DAOQOU010000000
VERSION     DAOQOU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_7125_cov_5.078925"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PLM90_12050"
     CDS             <1..1334
                     /locus_tag="PLM90_12050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ90158.1"
                     /translation="KYTHYMNQRPASTVIFADKFRYMTYRYSDNSLPMVLIIGAGFAG
                     LELIKQFNNKPFRVLLLDKNNYHTFQPLLYQIASAGLTAESIAYPVRRKIGPYPNIAF
                     RVAEVSSVDTSGQLVHTDIGEFHYDYLIIASGASTNFYGNNSLASTTFPLKSVQDALN
                     MRTTILQQFEKAVNASKTNGSKLLNFIIVGGGPTGVELAGALAEIKKNVVPSDYRELD
                     PEMMEVHLIEAAPRVLGTMSEKSSRLAKKYLEQLGVKVWLNAVVNSYENDILTLSNDT
                     KITAHHVIWTAGVKGVDIKGLEGNIRTRGNRIITDRYHRISGFENIYAIGDIASIQGD
                     EQYPQGHPGVAQVAIQQAHNLASNFIRNAKGKSIQAFNYHDKGSMATIGRHKAVVDLP
                     FISFGGYLAWYIWMFIHLMALVGFRNRFMVFMNWMWNYITYDRALRLILKK"
     gene            complement(1391..2044)
                     /locus_tag="PLM90_12055"
     CDS             complement(1391..2044)
                     /locus_tag="PLM90_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90159.1"
                     /translation="MRKLIFAICATLTGLFAFQSASAQLWLMTDGGVFFTKNLVGEGI
                     NAKAAWHFGDYDHNMVTFGGGYNLLGSSKVDIIVVDTAADVTDTIESTLTSNVITIDG
                     DYRRYFLGSDADDYFAFYGLGGLSLWMVNTKVKMGSYVDTVYMVEEGTKLVSSNMSVR
                     IPVGFGVDWTVRGRLWWYVEAKLELPITQVNNDFIGNDFGVSYHINTGARILLWDLF"
     gene            2198..2902
                     /locus_tag="PLM90_12060"
     CDS             2198..2902
                     /locus_tag="PLM90_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ90160.1"
                     /translation="MAKDTIIITDADGGLGSSVTSALLKAGYMLRASAMHEEAVERLK
                     SSYPEHYGNALTAVIADLGTEEGVKALLEGIPQPSGLVHLAGGYKPGNAVSDYSNDDF
                     SFLMNLNARPTFLLLKHLMPSLKALGKGSIVTIGAKPALHPVAANAVYTASKSAVIAL
                     TMAAAEEGRPFNVRANCIIPAALQTPGNLSWASKEQFETFTPLQDIADTILFLMSDEG
                     RGITGTTIPMYHKIAT"
     gene            2930..3646
                     /locus_tag="PLM90_12065"
     CDS             2930..3646
                     /locus_tag="PLM90_12065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ90161.1"
                     /translation="MLKDLHPEQLFFFDIETVPGAADYNELAPDLQQLFERKVGRQRN
                     EDENPAEFYFSRAGILAEFGKVICISCGYLQKKENEYHLKIKTFYGDDEKSLLEEFTQ
                     TLLNISSKGYQLCGHNIREFDVPWLCRRMLINGLILPPVLDIYGKKPWEVNHVDTLDL
                     WKFGDYKHYTSLNLLATILGVPTPKDDIDGSMVAEVYYKQHDLNRIAVYCSKDVVAVA
                     QVIMKLKGLPLIKSENISGI"
     gene            complement(3716..4147)
                     /locus_tag="PLM90_12070"
     CDS             complement(3716..4147)
                     /locus_tag="PLM90_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ90162.1"
                     /translation="MTLVKKGWSSPFSNKFLSDFFNADKLFEDDFFNVERMPAVNIVE
                     SADHFEVEVAVPGMKKEDFKVEVKDGMLHISAEREENKEEVKKNYTRKEFSVASFSRM
                     FTLPENVKDVDIGGEYKNGVLHIVLPKKEPVKIDAKKIEIK"
     gene            4324..5313
                     /locus_tag="PLM90_12075"
     CDS             4324..5313
                     /locus_tag="PLM90_12075"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HMZ90163.1"
                     /translation="MRSFLAMTFMLFLACPVFAGSDSLHAHIALPLPDKGIDDPIPQG
                     DFEVLVIPLKRVQNLYMVEAKVDSVVGNFILDTGAPHLVLNKTYFNTGKSAPGSTQYG
                     ITGGGNSVMNTTIDTLKVSELFYTRVDADIVNLGHLENARGVKILGLLGTSLFSRMEM
                     EIDMKNDLLYLYKTDEKGERLIPNNHTSSPDMQMPLDIVNDVLFIEGRIANKKLRFCL
                     DTGAERNVLSNTVSNKIISRFTLTKSSNLAGSGNSGQVVLNGVIDSVVTEGVSFTDMP
                     FILTNLSYLELVYGTTVNGVLGYDYLSKGYAVINMQKKLFSMYFYKPEEDDEP"
     gene            5300..6742
                     /locus_tag="PLM90_12080"
     CDS             5300..6742
                     /locus_tag="PLM90_12080"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HMZ90164.1"
                     /translation="MMNHNLSRILIFLMLLFPTVSNAQFAGSTVQVKGDMIIIRIDQK
                     NNDYNNVMLYFGLNEDSLFEYGNIGPLAAQGWELLDLTKRTAKIGRLITSSSSDIYWG
                     NQPFVINFPDVSNTMPGYPGDVVYGANSFIHKPSVFEDHSGNTVFYLPQHLNASTVYL
                     SGNFNDWSTGGTPMTKTDSGWMYSMKLEPGKYFYKFIIDGNWIHDVNNALRESDGHDG
                     FNSAYYVNNTVIRLKGFTDKKDIIFTGSFNNWNEHELHMIKTAGGWELPMFLKEGTYT
                     YKFIADGEWMLDPSNEIARPDGNGNVNSVMSVGDTTFFVLNGFEDAKIVILTGSFNNW
                     NTGELIMQKTSGGWQIPYVLAAGNYTYKYIVDGTWITDPSNPITSGQGDEINSVRTIK
                     PNHVFKLADYPTATNVYLSGSFINWAEPGFKMTKVDGVWKIPVFLQPGKCTYKFIVDG
                     NWITDPANPQTEQNEYGTGNSVIWKEVNAQ"
     gene            6835..>7125
                     /locus_tag="PLM90_12085"
     CDS             6835..>7125
                     /locus_tag="PLM90_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ90165.1"
                     /translation="MSAKEILLDVRNLATTFTTENGVVKAVDDISFQIYRGETVGIVG
                     ESGSGKSVTSLSLMRLIPDPPGRITGGQIFYHEAGQQPVDLVTVPDAAMRHYR"
BASE COUNT         2161 a         1474 c         1508 g         1982 t
ORIGIN      
        1 aaaaatatac acattacatg aaccaacgcc cggcgagtac ggttatcttt gcagacaaat
       61 tcaggtatat gacctatcgc tacagcgata acagtcttcc gatggtgctc atcattggcg
      121 ccggtttcgc cggtctcgag ttgattaagc aatttaacaa caagccattt cgcgttctgc
      181 tgctcgataa aaataattat cacacctttc aaccactgtt gtatcaaatt gcatcggccg
      241 gcttgacggc agaaagcatc gcatatcccg ttcgccggaa aattgggcca tatcctaaca
      301 tcgcttttcg tgttgcagag gtatcgtcgg tagatacttc ggggcaattg gtgcataccg
      361 acatcggcga atttcattac gattatctca tcattgcttc aggcgcaagc acaaattttt
      421 atggtaataa cagtttagca tcaacaacat tcccattgaa atcggtgcag gatgctttga
      481 acatgcgcac gacaatttta caacagtttg aaaaagccgt taatgcctca aaaaccaacg
      541 gcagcaaatt attgaatttt attatcgttg gtggcggacc gacaggagtt gagcttgcag
      601 gcgcacttgc cgaaatcaag aaaaatgtag tgccatcaga ttaccgtgaa cttgatcctg
      661 aaatgatgga agtgcatttg atagaagctg cgccgcgagt gctaggtacc atgtctgaaa
      721 aatcgtcgcg actagcgaaa aaatatcttg agcaattggg tgtgaaagta tggcttaatg
      781 cagtagtgaa ctcatacgaa aatgacattt taaccttgtc taacgacaca aagattaccg
      841 cgcatcatgt aatatggaca gcaggtgtta aaggagttga tatcaaaggg cttgaaggaa
      901 atattcgcac tagaggcaat cgtataataa ccgataggta tcatcgaatt tccggatttg
      961 aaaacattta tgccattggt gatattgcga gcatccaggg tgatgaacaa tatccccaag
     1021 gacacccggg ggtggcgcag gtggcaatac agcaagcaca taatcttgcc tcgaatttca
     1081 ttcgaaatgc aaagggaaaa tctatacagg catttaacta tcacgataaa ggcagcatgg
     1141 caacgatagg ccgacacaaa gcagtagttg atttgccatt tatttctttc ggaggatatt
     1201 tagcgtggta tatctggatg ttcatccacc tgatggcatt ggtaggcttc agaaaccgat
     1261 ttatggtgtt tatgaattgg atgtggaatt acattacgta cgacagagca ttgcgactta
     1321 tcctaaagaa atagcaatct ctgtcgcgtt gcgattagtt gttacccaat aaatcaagct
     1381 gatccgggaa tcagaacaag tcccaaagaa ggatacgtgc gccggtattg atgtgatagc
     1441 ttacgccaaa atcgttgccg ataaaatcat tgttcacctg agtgatcggc aattccaatt
     1501 ttgcttcaac ataccaccaa aggcgacctc taacggtcca gtcaacacca aaacctacag
     1561 gtatgcgaac actcatattt gaagacacta atttagttcc ttcttcaacc atatatactg
     1621 tgtctacata tgaacccatt ttcactttgg tattcaccat ccataaactt aatccaccga
     1681 gaccatagaa tgcaaaatag tcgtcggcat cagaaccgag gaaataacgg cggtaatcac
     1741 catcaatggt aattacattt gaagtgagtg tagattcgat ggtatcagta acatcggctg
     1801 ctgtatccac aacgataata tctactttag aagaacccaa taagttataa ccaccaccaa
     1861 aggttaccat gttatgatcg taatcgccga agtgccaggc agcttttgca ttaatacctt
     1921 cacctaccag gtttttagtg aaaaatacgc ctccatcggt catcagccat aactgggcag
     1981 acgcagattg gaatgcaaat aatcctgtca gagtagcgca aatggcaaag attaattttc
     2041 tcatatgggt gttgtttgac attccccgcc catgggagac ggcagaataa gcacaaaata
     2101 aaggtttttt accctatttg cgaaattccc cacccacaat aagggacact aatgtccaat
     2161 catgcacaca atcaggcgaa tatgtaaatt tgagcctatg gcaaaagata ccattatcat
     2221 tacggatgcc gatggcggac tgggaagcag cgtcacttct gccctgctta aagccggcta
     2281 catgctgagg gcaagtgcca tgcatgagga ggctgttgag cgactgaaaa gcagctatcc
     2341 ggagcattat ggcaatgcgt taacagcggt gattgctgat cttgggactg aagagggtgt
     2401 taaagcgctg cttgagggta ttccccaacc gtcgggactg gtgcaccttg caggagggta
     2461 caagcccggg aacgccgttt cagactattc aaacgacgat ttcagttttt taatgaattt
     2521 gaatgcaagg ccaacatttt tgttgctcaa gcatctcatg ccaagtttaa aagctttggg
     2581 aaaaggttct attgtaacta ttggtgccaa gcccgcacta catcctgttg cggctaatgc
     2641 tgtgtatact gccagtaaaa gtgccgtaat tgcattaaca atggcggctg cggaagaagg
     2701 aagacctttt aatgtgcgtg ccaattgcat cattccggct gctttgcaaa cgcccggaaa
     2761 tttaagttgg gcctctaaag aacagtttga aacattcacc ccattgcaag atattgcaga
     2821 tactatttta ttcctgatgt cggatgaggg acgcggaatt accggcacaa ccataccgat
     2881 gtatcataaa attgctacat aattttttca tcattgttta ttccttttta tgcttaaaga
     2941 cttacaccct gaacaactgt tttttttcga cattgaaaca gttcccggtg cagccgatta
     3001 taatgagctg gctccggact tacaacaatt atttgaacgc aaggttggcc gacaaagaaa
     3061 tgaagatgag aaccccgcgg agttctattt ttcacgcgcc ggaatacttg ccgaatttgg
     3121 aaaggtaatc tgcatctcat gcggatatct tcagaaaaaa gaaaatgaat atcacctcaa
     3181 aataaaaacg ttttatggtg atgacgaaaa gtcactgctg gaagaattta cgcaaacatt
     3241 gctgaatatt tcttccaaag gctatcaatt atgcggacat aatattcgcg aatttgacgt
     3301 gccatggctt tgcagaagaa tgcttatcaa tggtttaata ttgcctcctg tgcttgacat
     3361 ttatggcaag aaaccatggg aggtcaacca tgtggataca ctggatctgt ggaagtttgg
     3421 cgattataaa cattacacct cactcaattt gctggcgaca atactcggtg taccaactcc
     3481 aaaagatgat atcgacggaa gcatggttgc cgaagtatat tataagcaac acgatctgaa
     3541 tcgcatagct gtttattgca gtaaagatgt tgttgccgtt gcccaggtaa ttatgaaact
     3601 caaaggttta ccactcatca aaagcgaaaa tatcagcggc atttaaaaaa tacggggcga
     3661 tgtgccatca agtacatcgc cccgttacat acctcagaac agactacgcc gacgcctatt
     3721 tgatctcaat ctttttggcg tcgattttaa caggttcttt ttttggaaga acaatatgga
     3781 ggacaccgtt tttgtactcg ccgccgatgt cgacatcttt aacattttca ggaagtgtga
     3841 acatgcggct gaaggatgct acgctgaatt ctttgcgcgt ataatttttc ttcacctctt
     3901 ctttgttttc ttccctttcg gcagagatgt gcaacatgcc atcttttacc tctactttaa
     3961 aatcttcttt tttcattccc ggaacagcca cttctacttc gaagtgatct gcactctcca
     4021 caatatttac cgcaggcatt ctttcaacat tgaaaaaatc atcttcaaat aatttatcgg
     4081 cattgaaaaa atcgctgaga aatttattgc tgaatggaga gctccagcct ttttttacca
     4141 gtgtcataac tcagttttta agtggtgaac tgaatattgt tgacattgca attataccca
     4201 cataaacgaa gcaaaaacag tgatgttggt taccgaaaat tgaaattgta atcaattagt
     4261 cgcccggtaa tccccgtcac cctcggcaaa gagcatctta aaccgtattt ttgcccatag
     4321 ggtatgcgca gttttcttgc catgacattc atgcttttcc ttgcctgtcc ggtgtttgcc
     4381 ggctcagatt cattgcatgc gcatattgct ctcccattac ccgacaaggg cattgatgac
     4441 cctattccac agggtgattt tgaggtattg gttattccat taaaacgcgt tcaaaatctt
     4501 tatatggtag aggcaaaggt ggatagcgtt gtaggcaatt tcattctcga taccggagcc
     4561 ccgcatttag tgctgaacaa aacatatttc aataccggaa aatcagcacc gggttctaca
     4621 caatacggca ttacgggagg tggaaattca gtgatgaata ctactatcga cacccttaag
     4681 gtaagcgagc tgttttacac gcgtgttgat gcggatatcg ttaacctcgg acatcttgaa
     4741 aatgcccgtg gggtgaagat tctaggtcta ttaggtactt ccttattcag ccggatggaa
     4801 atggagattg atatgaaaaa cgacctgctc tatttgtaca aaactgatga aaaaggtgaa
     4861 cgcctcatcc ccaataatca cacttcgtca cccgacatgc aaatgccact tgatatcgtc
     4921 aatgatgtgt tatttattga aggtcgcatt gccaataaaa aattgcgatt ttgcctggat
     4981 accggtgcgg aacgaaatgt gctcagcaat acagtaagta acaagataat aagccgattt
     5041 acccttacca aaagcagcaa cctggcaggc tcgggcaaca gcggacaagt agtgctgaac
     5101 ggagtgattg attctgttgt tacagaaggg gtgtctttta cggatatgcc attcattcta
     5161 accaacctta gctaccttga actcgtatat ggtactacgg ttaatggagt actgggttac
     5221 gactacctgt cgaaagggta tgccgttatc aatatgcaga aaaaactttt ttccatgtat
     5281 ttttataaac cggaggaaga tgatgaacca taatttgtcg aggatattaa tctttctgat
     5341 gctgcttttt ccaacagtat caaatgcaca atttgcaggc agtactgtgc aggtaaaagg
     5401 cgatatgatc attattcgca ttgatcagaa aaacaatgat tataacaatg tgatgctgta
     5461 tttcggttta aatgaagata gtttattcga atatggcaat attggacctc ttgcagctca
     5521 aggatgggaa ttattggact tgactaaaag aacagcaaaa ataggtcgat tgattacctc
     5581 atcaagcagt gatatttatt ggggcaatca accctttgtt atcaattttc cggatgtgag
     5641 taatacaatg cccggctatc cgggtgatgt ggtgtatggc gctaattcct ttatccataa
     5701 accaagtgtg tttgaagacc attcaggcaa tactgtattt tatcttcctc aacatctaaa
     5761 tgcttcaacg gtatatctca gtggcaattt caacgactgg agcaccggtg gaacaccaat
     5821 gacgaaaaca gattccggtt ggatgtattc catgaaattg gaacccggaa aatatttcta
     5881 caagtttatt attgatggaa actggataca tgatgtaaac aatgcactcc gcgaaagcga
     5941 tggtcacgat ggatttaata gtgcgtatta tgtaaacaat accgtgatca gattaaaagg
     6001 atttacagat aaaaaagata tcatattcac aggttctttc aataactgga atgagcatga
     6061 actccatatg atcaaaactg ccggcggatg ggagctgccg atgttcctga aagaaggaac
     6121 ttacacatac aaattcattg ccgatggcga atggatgctc gatccttcaa atgaaatcgc
     6181 cagacctgat ggcaacggca atgtgaattc cgttatgtcg gttggcgaca ctacattttt
     6241 tgtgttaaat ggttttgagg atgcaaaaat tgtaattctt accggcagct ttaataattg
     6301 gaataccggt gaactcatca tgcaaaaaac atccggtggt tggcaaatac catatgtgct
     6361 tgctgcggga aattatacct ataaatatat tgtcgatgga acatggataa cagatccgtc
     6421 aaatccaatt acatcaggtc agggcgatga aataaattct gttcgaacca tcaaacccaa
     6481 tcatgtattt aaacttgccg attatcccac agcaactaat gtatacctat ccggaagctt
     6541 tatcaattgg gcagaaccgg gcttcaaaat gaccaaggtt gatggtgtgt ggaaaatccc
     6601 tgttttcctg caacccggaa aatgcactta taaatttatt gtcgatggca actggatcac
     6661 cgatccggca aaccctcaaa cagaacaaaa cgaatacgga acaggaaatt cggtgatatg
     6721 gaaggaagtc aatgcccaat gatttgaagg atgcaacaat aaaacattta taatcaattt
     6781 gagcttaatt cctcgcaaaa tacaattagc aattaaacac taacttgcag cattatgtcg
     6841 gcaaaggaga ttttactgga cgttcggaat ctggctacca catttaccac cgaaaatggt
     6901 gttgtaaagg ctgttgatga tatctcattt caaatctata gaggtgaaac agtaggtata
     6961 gttggtgaaa gtggctccgg aaaatctgtt acctcactat cactgatgcg attaattccg
     7021 gatccaccgg gacgaataac aggagggcaa atattttatc acgaagcggg acaacaacct
     7081 gtcgatcttg tcaccgttcc tgatgcagcc atgcgtcatt atcgc
//
LOCUS       DAOQOU010000165         7097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_5965_length_7097_cov_8.079381, whole genome shotgun sequence.
ACCESSION   DAOQOU010000165 DAOQOU010000000
VERSION     DAOQOU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5965_length_7097_cov_8.079381"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLM90_12090"
     CDS             <1..839
                     /locus_tag="PLM90_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ90166.1"
                     /translation="ADGDTYGDPFIDSTSCSMLIGFVENVLDCNDTDATINPDAIELC
                     NSIDDNCNTFIDEGLAIYTFYLDADGDTYGDPATALDTCFEAITGYVLNNFDCNDSNS
                     SIYPGAEEICNYLDDDCDGVADDNLAYTWQYQDADGDNYGNVVIDTLACLDIPGYVTD
                     STDCNDLNSNIYPGAPELLNGADDDCDGQNDEGLSIAEIHLDNISIYPNPSNGIVTIE
                     LYSPQAINMELRTIDGKLVNTILNVTEDKYTIDLSNEASGIYLLYIESREWTQTVPLV
                     KE"
     gene            complement(937..1284)
                     /locus_tag="PLM90_12095"
     CDS             complement(937..1284)
                     /locus_tag="PLM90_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMZ90167.1"
                     /translation="MGTINATINYKGKSGKNYQFRLYTINSEFAENSGGVYIITKRTE
                     NPDGNGTHKLLYIGKANKFQSRFSNHEKWDAFMKNDANCVGIYKTSSETESLDIEEDL
                     IKEQSPLLNSQHN"
     gene            complement(1329..1547)
                     /locus_tag="PLM90_12100"
     CDS             complement(1329..1547)
                     /locus_tag="PLM90_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90168.1"
                     /translation="MESKQKIILLKKQSNFLLMQPETGMGFQIVDINTKAYGWIYNVV
                     ALNSEFIYIDSIYKITANDIKEIKMSQS"
     gene            complement(1537..2232)
                     /locus_tag="PLM90_12105"
     CDS             complement(1537..2232)
                     /locus_tag="PLM90_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90169.1"
                     /translation="MVFRAINIDTTKLLSLPESGMGYQVIQDMRPNNMAKYLVFNAEL
                     ILPMDGTLSQNIQAIRQRGYDQLLRNAINFSVPTNNYKLLTGLEYRTGILNLTDSLIN
                     YRQRHRYGTAAKDNPSEKANGHEVFVRVSAYEKDKRIDFINRKLLPGTYTTTLSDYTI
                     CVNTNDNPIDRYALPSDEEIRWAFYIQPKSWDYLKRGVVQPAFGHHGGGLEALFESGT
                     SVGTYLHTTAYGK"
     gene            complement(2603..5542)
                     /locus_tag="PLM90_12110"
     CDS             complement(2603..5542)
                     /locus_tag="PLM90_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90170.1"
                     /translation="METLLNLNYKFRLMVICSLSSLLFAFSKPIINNDVNSRFSSESS
                     VLSMLPDSTVVDTMQYEDGDTITFYVSGQDTTFITYQSESEDSEDMELFTISASNPKI
                     IVQLSDHSGEVNMGLIGINLTDIFEPLKTPQDALTNYTYSDDPWEILVDLAPSTIRIF
                     SGASAKFMHPMGGKLKDAVTNEYYGGYGYDWKEMLTYYDITDNILNAPTLAGSPNWAL
                     IETQMAFNGSGLTWVSSNNRKRYSEFYDKCMSQPTYNPALPEYDTPEERPLYINQLID
                     LVDAIETGNPGHEVNVIYCVNIESGTAEEMTRTIDYLESHGVHVSGIELGNEVMSEFH
                     YQGLGFIDFEHYWKYINGDNYDAVLVDGDYNTSVLESVLPDEVETDHNYIQAIKGDLS
                     FYQIKIGLPAANTPNCGASYDFPLMPQQGGEEQSRDVLAGPPVITVPDPTDPDPCECE
                     YPQWNVDMQTYFDEYTTSESVSFAYKFDAVILHTYYTTTNNNPDCTVNSNWQEIMLPL
                     HPSYPEISTDLDGYSFGVLQHTDGEWTYSTLDTRLSTVFNDITGVHTPLATSPLIPGN
                     FKEFTRDRLDISFTEHANWLDFLDTDDGPETKEIWITEYNIDEGVNIMFDPLIDDADD
                     IAAANETSFKNYTGAVANTFSHAFVMHNWFLWMLKANFDNDYRNGFLTTATLQNYLGG
                     SPTMMTTKYAQSDIVGTGVGGASCATDVLEPYFIRRTTSFPPELWRVINDNDLKYLRS
                     NSVIGAQNDNLAPTVFIDDNVEDPKLYVFYSNVKPGTQWFGFDPGMLDDYFDLTGEYV
                     DLTNSMTGSALIADQLYSTSGDNPLFGINDQYNTCANVIDNENYYELTTLETISPNVT
                     CPTLFATEFPAGVCAEVPGRSIGYFVVPIDFLPLKKGDPDEIFIVYPNPTSNYFTIQQ
                     KDVTSDVLSNITVELFTSQGDLALTYTNVAESERISIESLPVGVYMVKITYGNNQIAN
                     ESIIKMK"
     gene            5609..>7097
                     /locus_tag="PLM90_12115"
     CDS             5609..>7097
                     /locus_tag="PLM90_12115"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HMZ90171.1"
                     /translation="MNFYKLLIAKYLAICLGFSPVVFGQAPEIEWQNWLGSNGDERPW
                     NMVETDDGGFLIVGEANYADGAVTEAFGIVDYWVVKLSSDGALEWQKSYGGSHVDVCF
                     GVCNGNDSGFLMAGYTYSTDGDISTPLGGADFWIVKIDNEGNIEWEKSYGGSSNDVAH
                     VVKQSIEGGYVVAGTSASFDGMVTGYHGGSGVDTWVVKIDDYGNLIWESCFGSMEQEY
                     CNEVIQSADGNYYISGSTEGNNGDIVGNHGESDGLILKVNQEGELIWSKCYGGPDWEY
                     DFNILEIAPEKFILAGVTDGPGGQVDDFNGGINDVWVICIDSNGVLLWSENYGGSEAD
                     QYSSISKNQNHELIITSASNSSDFDVSSNYGWSDYWVFCIDTLGVLQWQKSFGGSDVD
                     VPTSILGIDENKIVVTGYTGSDDFDVEADSFTYAFNYWTIQLAFCEERYFADFDNDGF
                     GDIASDTIACNPPTGYVTDSTDCNDSNPDIHPLLSDICNSIDDNCN"
BASE COUNT         2162 a         1224 c         1404 g         2307 t
ORIGIN      
        1 atgcagacgg tgacacatac ggtgaccctt ttatagattc tacatcatgt agcatgttga
       61 tcggatttgt agagaatgtt ttggattgca atgatactga tgcaacgatt aatcccgatg
      121 caattgaatt atgtaacagc atagacgaca actgcaatac tttcattgat gaaggattag
      181 ctatttatac tttctattta gacgcagatg gagatacata tggtgatcct gcaacagctt
      241 tagacacctg ctttgaagcc ataacaggtt atgtgttaaa taactttgat tgcaatgact
      301 ccaactctag catctaccct ggcgcagaag aaatctgtaa ttatttagac gatgattgcg
      361 atggcgtggc cgatgacaat ctcgcataca cctggcaata tcaagacgct gatggagata
      421 attatgggaa tgtggtaatc gacactctgg cctgtcttga tattcctggc tatgtcacgg
      481 attccactga ctgtaatgat ctcaattcaa atatttatcc cggtgctcca gaactgctca
      541 acggtgctga tgatgattgc gatggtcaaa acgacgaagg gctctctatt gcagagattc
      601 atttagataa tatctccatt tatccaaacc catcaaacgg tattgttaca attgaattgt
      661 attcaccaca agccattaat atggagttga ggaccattga tgggaaactg gttaacacta
      721 ttttgaatgt tacagaagac aagtacacaa ttgacctatc taatgaagct tcaggaatct
      781 atttactata catagagagc cgagaatgga ctcaaacggt tccattagta aaggaatgag
      841 ggtgtaagcg ttatttttaa tcactatata gtctttgaat gcttataaaa taatgtaata
      901 ccaaaggcat tctccactct cacttaaccg aatgaactaa ttgtgttggc tgtttaataa
      961 aggcgactgt tcctttatta aatcttcttc aatgtctagg gattcagttt cacttgatgt
     1021 tttataaatt cccacacaat ttgcatcatt cttcatgaac gcatcccact tttcatggtt
     1081 gctgaatcga gattgaaatt tgtttgcttt accaatatag agtaatttgt gcgttccgtt
     1141 tccgtctgga ttctctgtcc tctttgttat aatatacact ccaccagaat tttcagcaaa
     1201 ttcagagttg attgtataca agcgaaattg atagtttttg ccagattttc ctttatagtt
     1261 aatagttgca tttattgttc ccataattaa acttgattta tttatttaaa cggaaataat
     1321 tgaaacattc atgactgcga catcttgatt tctttaatat cgtttgccgt tattttataa
     1381 atgctatcaa tatagataaa ctcagaattt aaggctacga cattatagat ccaaccgtag
     1441 gctttagtgt taatatcaac aatttgaaac cccatacctg tttcaggttg cattaataag
     1501 aaatttgatt gtttctttag cagtataatt ttttgcttac tttccatagg ctgtagtatg
     1561 cagataagtt ccaactgaag tgcctgattc aaatagagct tctaatccac ctccatgatg
     1621 tccgaaagca ggctggacaa caccacgttt taaataatcc caagacttag gttggatata
     1681 gaaagcccac ctaatctctt catcacttgg taatgcatat ctatcaatgg gattatcgtt
     1741 agtgttaaca caaatagtat aatcagataa agtggttgtg tatgttcctg gaagaagttt
     1801 cctatttata aaatctatcc ttttgtcttt ctcatatgcg gaaactctta caaaaacttc
     1861 atgaccgtta gccttttcag aaggattatc ctttgcagca gtgccatatc tatgtctttg
     1921 tctgtaattt attagactgt ctgttaggtt tagaatacct gttctgtatt ccaaaccggt
     1981 taataattta taattattag ttggaacaga aaaatttatg gcattgcgca ataattgatc
     2041 atatccacgt tgtcgtatag cttgaatatt ctgagaaagt gtcccatcca tgggtagaat
     2101 cagttcagca ttaaatacaa gatactttgc catattattt ggacgcatgt cttgtataac
     2161 ttgatatccc ataccagatt ctggaaggga taatagctta gttgtgtcaa tatttattgc
     2221 tctaaatacc atatgatagt gctatttatt aattggggaa ttgaaaattt ataggcaaga
     2281 ttctttcgtt tttagaaatg aacaattgct aaccaagtaa ataataagaa ttttgcaaca
     2341 atttatgaaa aaactttagt tacattatgt tggcattgga tgtcaaactc ctaattcaca
     2401 gggttgggaa atggggttct gatttatata tggtcaatat ttcgttttgt atattgcgaa
     2461 caaaaatctc ttcgattttt atttattaaa tggcaattat ttgaaaatag gcaaaggttg
     2521 cagaaggact ctgccccctt tgccatctcc atgaaaacaa atctttctaa ttatgaatat
     2581 tgtacgattt taataaacca acttacttca tttttattat ggattcattg gctatctgat
     2641 tattaccata ggtgattttt accatatata caccaactgg cagtgattca atactaattc
     2701 gctcggattc tgcaacgttg gtatatgtta gagctaaatc accttgagag gtaaataatt
     2761 ccacagtgat attgcttagc acatcagaag ttacatcctt ttgttgaatt gtgaagtaat
     2821 tagatgtagg gtttggatat actataaaaa tttcatctgg gtcacctttt tttagtggaa
     2881 gaaagtcaat tggcactaca aaatatccga ttgaacgtcc tggaacctca gcgcaaactc
     2941 ctgctggaaa ttctgtggca aacaacgtgg gacaagttac attaggactt attgtttcaa
     3001 gtgtggtcaa ctcataataa ttttcattgt caataacgtt tgcacacgtg ttatattgat
     3061 cattaattcc aaataatgga ttgtcgccag aggttgaata tagttgatca gcaatcaatg
     3121 ctgaccctgt catagaatta gttaagtcaa catattctcc tgttaggtca aaataatcat
     3181 caagcattcc tggatcaaac ccgaaccact gtgttccagg ttttacattc gaataaaaca
     3241 cgtataattt ggggtcttca acattgtcgt caataaagac tgttggagct aagttgtcat
     3301 tctgggctcc gattacagag tttgatctta aatactttaa gtcattatca ttaatgacac
     3361 gccatagttc tggggggaat gaagtagtcc gcctaataaa atacggctca agaacatcag
     3421 ttgcacatga tgcacctcct acgccagtgc cgactatatc gctctgggcg tatttggttg
     3481 tcatcattgt gggtgaacca ccaaggtaat tttgcaatgt ggcagtagtc aaaaatccat
     3541 tacggtagtc attgtcgaaa ttggccttaa gcatccacaa aaaccaatta tgcatcacaa
     3601 atgcatggga gaaagtgttt gctactgctc cagtataatt tttaaatgag gtttcatttg
     3661 ccgcagcgat atcatcagca tcatctataa gtgggtcaaa catgatattt actccctcat
     3721 caatattgta ttcagttatc caaatttcct tagtttctgg gccatcgtct gtatctagaa
     3781 aatctaacca gtttgcatgt tccgtgaagg agatatcaag gcgatctcgt gtgaattctt
     3841 taaaattccc aggtattaat gggctcgttg ctaaaggtgt atggactcca gttatatcat
     3901 taaaaacagt actcagtcga gtgtctaaag ttgaatatgt ccattccccg tcggtgtgct
     3961 gaagcacgcc aaaggaataa ccatctaaat cggtgcttat ttctggatat gatggatgaa
     4021 gtggtagcat aatctcctgc cagttagaat taactgtgca atcaggatta ttgtttgtag
     4081 ttgtatagta ggtgtgtagt atgaccgcat caaatttata tgcaaatgaa acggattcgc
     4141 tagtagtata ttcatcaaaa tatgtttgca tgtctacatt ccattgcggg tattcgcatt
     4201 cacatgggtc tgggtcggtt gggtcaggaa ctgtaattac gggaggcccg gctaatacat
     4261 cacgcgactg ttcctcgcca ccctgttgtg gcataagggg gaaatcataa ctagctccgc
     4321 aatttggagt attggctgca ggtaacccaa tttttatttg ataaaatgat aaatctcctt
     4381 taatcgcttg aatgtagttg tgatcggtct caacttcatc tggtagcaca gattcaagga
     4441 ctgaggtgtt ataatcacca tcaaccagca ctgcatcata attatcacca ttgatatatt
     4501 tccagtaatg ttcgaaatcg ataaatccta ggccttgata atgaaattca ctcatgactt
     4561 cattgcctaa ttcgattcct gatacgtgaa ctccatgaga ttcaaggtag tcaatagttc
     4621 tagtcatttc ttcagcggtg ccagattcga tattcacaca ataaataacg tttacctcat
     4681 gtccaggatt tccagtttcg attgcatcaa ctaagtcaat taattgattt atgtataatg
     4741 gtcgttcttc gggtgtatca tactctggca aggcaggatt ataagtcggt tgcgacatac
     4801 atttatcata gaattctgag tatctctttc tattgttaga actaacccaa gttagtccac
     4861 taccattgaa agccatttgt gtttcaatta gtgcccaatt tggtgaacct gccagagtag
     4921 gtgcattaag gatgttgtca gttatatcat agtaagtgag catttctttc caatcatatc
     4981 cataaccacc ataatattca ttcgttacag catctttcaa tttaccaccc attggatgca
     5041 taaattttgc actagcaccg ctgaaaattc ttattgttga aggtgccaaa tccactaaaa
     5101 tttcccacgg atcatctgaa tatgtataat tagttaatgc atcctgagga gtctttaatg
     5161 gttcaaaaat gtcggttaag tttatcccta ttagacccat attcacttct ccagaatgat
     5221 cagacaattg aacaatgatt ttagggtttg atgcacttat ggtaaataat tccatgtcct
     5281 ctgaatcctc agactcagat tgataggtaa taaaagttgt atcctgacct gaaacgtaaa
     5341 aggtgatagt atcaccatct tcatactgca ttgtgtcaac caccgttgaa tcaggcaaca
     5401 tgcttaaaac tgaactttcc gatgaaaatc gggagttaac atcattgtta ataatgggtt
     5461 tggagaaggc gaacagcaat gagctgaggg aacaaataac cattaagcgg aatttgtagt
     5521 ttaggtttaa taaggtttcc ataagaatca tttttttcga aaatagaaga ataattacta
     5581 ttttgcaagc cctaaatgaa aacaaatcat gaatttttat aaacttctga tagccaaata
     5641 cttggcaata tgccttggat tcagcccggt agtatttggt caggcacctg agatagaatg
     5701 gcaaaactgg ttaggttcaa atggtgatga gcgcccctgg aatatggtcg aaactgacga
     5761 tggtggtttt ttaatagtag gcgaggctaa ttatgctgat ggcgcagtaa cagaagcatt
     5821 tggaattgta gattattggg ttgtaaaact tagtagtgat ggtgcactag aatggcagaa
     5881 atcttatggt ggatcacatg ttgatgtatg ctttggcgta tgtaacggaa atgatagtgg
     5941 gtttctgatg gccggttata catattctac cgatggagac atttccacac ctttaggtgg
     6001 ggcagatttt tggattgtaa aaatagacaa tgaaggaaat attgaatggg aaaagtcata
     6061 tggagggtct tcaaacgatg tcgctcatgt tgtaaaacaa tcaatagagg gaggttacgt
     6121 agtagccgga acaagtgcct catttgatgg catggttaca ggttatcatg gtggatcagg
     6181 agtagatacc tgggtcgtaa aaattgatga ttatggaaat ctgatctggg aatcatgttt
     6241 tggttctatg gagcaggaat attgtaatga agtaatacaa tctgcagatg gaaattatta
     6301 tatttctggg tcaacagaag gaaataatgg tgatattgtt ggtaaccatg gggagtctga
     6361 tgggttaata ttaaaagtca atcaggaagg tgaattaatt tggtcaaaat gttatggagg
     6421 tccagactgg gagtatgatt ttaacatcct tgaaatcgcg ccagagaaat tcattcttgc
     6481 aggtgtaaca gatgggccag gaggacaggt ggatgatttt aatggaggga taaatgatgt
     6541 ttgggtaatt tgtatagatt caaatggggt attattatgg tcagaaaatt atggagggag
     6601 tgaagcggat caatatagtt caattagcaa gaatcaaaat cacgaactta ttattacatc
     6661 tgcatctaat tcctcagact tcgatgtttc aagcaattat ggctggagtg attattgggt
     6721 tttttgtata gatacacttg gtgtattaca gtggcagaaa tcatttggag gaagtgatgt
     6781 agatgtgcca acatcaattt taggaataga cgagaataaa attgtagtta cgggttatac
     6841 aggctcagat gactttgatg ttgaagccga tagttttaca tatgccttca attattggac
     6901 aatccaatta gccttttgtg aggaaaggta ttttgcagat tttgacaatg acggttttgg
     6961 tgatatcgct tctgatacaa ttgcttgtaa cccaccaacc ggttatgtca cagacagtac
     7021 cgactgcaac gactcaaatc cagacatcca tccgttactc tcagatatat gcaactccat
     7081 tgacgacaac tgcaatg
//
LOCUS       DAOQOU010000166         7056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6024_length_7056_cov_5.442223, whole genome shotgun sequence.
ACCESSION   DAOQOU010000166 DAOQOU010000000
VERSION     DAOQOU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6024_length_7056_cov_5.442223"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1820)
                     /locus_tag="PLM90_12120"
     CDS             complement(138..1820)
                     /locus_tag="PLM90_12120"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HMZ90172.1"
                     /translation="MGLNIGLLLGGIGVFVFGIQLLEKALRSLMSRRFKLFLRRQTGR
                     PFRAVFGGTVLSAALQSSSVVNFLVLAFAGSGIISLKNALAVIMGTNLGTTLNSWIVA
                     TIGFRLEIEMYALPVAGVAGLLLFVFPEKKFSSIARFFMGFSFLFIGLGFMQQSVQDE
                     AIIQAMSRFRDSGAFVFLILGFIATTITQSSAATVAITLSLLYASAVGFSDAMAIVIG
                     SEVGTSVKLILGALDGVPVKKRISVGNFMYNLVTTIVAFALLRPAEICITEIMHVRDP
                     LIGLAVFQTGMNLMSIILFFPFLSAFSNRLEKSFNTEPAIASLFITPHTPVIPDGGLE
                     LLKKETGYFIHACMSFNAHNFHLHDTEIPMMPEFAEILHQRNTEQQPLALRYMLLKEQ
                     YGEIQSYYIRLKSQNLEPDDAIMVEQLIAGVRSAMYASKCIKDLFDDLHEWQNSADEQ
                     QYGFLKNMRIDVRHFYMQLTLNKWQSAEGDVVSDELLALLKHAQQTYQHNIEDIYRSM
                     SEHHISDTELATRMNFNRELFTSSKSMIMSCRSYLLDPLRAEKFNEIPTYIP"
     gene            1952..5365
                     /locus_tag="PLM90_12125"
     CDS             1952..5365
                     /locus_tag="PLM90_12125"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMZ90173.1"
                     /translation="MKSFKSINNLTGWIVFGIALFVYASTMEATASFWDCGEFIAATY
                     KLQVVHPPGAPIFLMLGKIFSLFASTKEQVPVMTNFFAALSTSFAILFTFWIITRLIK
                     RIMSPNDEPTFSQTIVIILSGVIGALVCLFMDSMWFSAVESEVYALATFFFVIIFWSM
                     VKWEAHAGDPNSDRWILFMALLMGLSIGVHLLALLVVPAIALIYYFRNYKYTFKGLMY
                     ALLIGAGLVAFLLYGLLDKFIGLGAWMDRVFNNFGAPFGTGLIIYGIMIVGGVVWGIR
                     KAIEKSNRVLYLALMSFTFLTIGLTSYAMVLIRAEANPVINMNGINDVHSFLSYLKRE
                     QYGSRELLYGPFWTAQPVEYEKGKAKWGKVEGNDKYQIISHEYKPVYKIPSEYANSPE
                     AQLLIQRNRQILFPRMGSLEDRHAGLYYNFANVPQGQEETYVPTGGDNMRFFANYQLG
                     HMFWRYFMWNFSGRQNDSQGNFYDLYKDGNWVTGITAIDKSKNPQLVDVPAELSDIDS
                     HNVYYMLPFIIGIIGMVYQLRNDKRGFAIVFMLFLFMGFMNIINMNQPPTEPRERDYA
                     QIGAFFAFALWVGLGLAGIVDMAKNFASKKVLQEYALYSLLILLVMYFTGLTMYKFTT
                     IAYVMIYTVGFMFVVGFIGSLIYKATKKELTVGIVAFLMLAPAPYLMATQNWDDHDRS
                     DRTFARDIARNYLESCPPNAILFTQGDNDTYPLWYAQEVEGIRTDIRIVNLSLLGVDW
                     YINQLRNATNDSKPIALTLGPEKILGDKRNQIVSNDKSKFKNTTMDINDFLNFVGQDD
                     PKYQIYSEGLEDYFSYMPTDKLKISIDNEAMKSQGIVPESLQGDMRKEISFKLTNKTL
                     LKNDLMTLDIVANNINKRPICFAVSVTGESHLGMQKYLMQRNMVYQLMPVEMENAKGV
                     MDGFQKGIETDIMYDQLINQKDKFTYGGVEKGKPIYMDPSAYGSILTAKYLNYLDLVK
                     SLNDERAYLEAQARQMMKDTVNQEYTEVGQELMKESAAKKEKMITVLDQMMVLFPDSS
                     VPYDNNMVNVAQLYQQLGKPEKASEIINTMMPRTFSELEYYYQMARNSTATAQMYQDD
                     QMRSEQTVYLGIEILKKSDAKDQADTYDKQWEALRMKYGIPDLNERRRR"
     gene            5346..6110
                     /gene="rlmB"
                     /locus_tag="PLM90_12130"
     CDS             5346..6110
                     /gene="rlmB"
                     /locus_tag="PLM90_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMZ90174.1"
                     /translation="MREGEGNFKRKKFDNLVFGRQPVVELLMRESPVDKILLTKTAQG
                     DIIPQIKHLAGEKQVPVQYVPVEKLNQLTGGNHQGVIAFTAAVAFQKIDDILPFIIEK
                     GEVPLILILDGITDVRNFGAIARTAYAGGVHAIIIPAHHAAPVNSDAMKTSAGALAEI
                     PICREKNYELVLKYLKLNGLHIIGADAHADKFIYNSDLTVPTAIIMGSEDEGISAISR
                     KHLSETVKIPMQNSFDSYNVSVAAGMIIYEVMRQRA"
     gene            complement(6253..>7056)
                     /locus_tag="PLM90_12135"
     CDS             complement(6253..>7056)
                     /locus_tag="PLM90_12135"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HMZ90175.1"
                     /translation="VSNIIQRGGTILKTARSERFMTAEGRKMAFDNLKLNGIEGFVAI
                     GGDGTFRGATVFEKEFGISCVGVAGTIDNDLYGTDYTLGFDTAINTAIDAIDKIRDTA
                     MSHNRLFFVEVMGRDAGFIALSTGIAGGAEAIVIPETETNIDYLISTLERGWLRQKSS
                     AIVIVAEGDERGGAFEIAKLVKERFNHYDTRVSVLGHIQRGGNPTVRDRILGSRLGMA
                     AVEALIEGRKNVMAGQVNDEVVFTPFANATKHHMEINKQMLQLVEILAM"
BASE COUNT         2121 a         1466 c         1420 g         2049 t
ORIGIN      
        1 cctttgttac ttgaattgca attacaacct ccggtaagtc ggcatgtcgc aagaatgcag
       61 gcggggctgc ttcaagtgcc ttggctaaag tgcgaatact tgaaaatact aaatcaaacc
      121 catttttgag atgagttcta aggaatatag gttggaattt cattgaattt ttcagctctc
      181 agcggatcga gcagataact gcgacaactc atgatcattg attttgagga ggtgaataat
      241 tcgcgattaa aattcatgcg cgttgccagt tctgtgtcac tgatatgatg ttctgacatg
      301 ctgcgataaa tatcttctat attatgctga tatgtttgtt gtgcatgttt caacaatgct
      361 aataattcat ctgaaacgac atcgccttca gctgattgcc atttatttaa tgtcaattgc
      421 atataaaagt gacgaacatc aatgcgcata ttttttagaa atccatactg ctgttcatca
      481 gcagaatttt gccattcatg caaatcatca aacaaatctt tgatacattt tgaagcatac
      541 atggcactgc gcactccggc tatcagttgt tctaccatga tagcatcatc aggttcgagg
      601 ttttgtgatt ttaatctgat ataataagat tgtatttcac cgtactgttc ctttaacaac
      661 atgtaccgta atgcaagtgg ttgctgttca gtattacgct gatgaagaat ctcggcaaac
      721 tcaggcatca tgggtatttc agtgtcatgc aggtgaaaat tatgggcatt gaaactcatg
      781 catgcgtgta tgaaatatcc cgtttctttc ttcaatagtt caagccctcc atcaggaata
      841 actggtgtat gcggcgtaat aaataatgaa gcaatagccg gctcggtgtt aaatgatttt
      901 tcaagcctat tgctaaaagc tgacagaaag ggaaaaaata aaatgatgct catcaaattc
      961 atccctgttt gaaatactgc caatccgatt aatggatcgc gcacgtgcat gatttctgta
     1021 atacatattt ctgccggtcg caataatgca aatgcgacaa ttgtagtcac taggttatac
     1081 atgaaattgc caaccgaaat tctttttttc accggtacac catctaatgc tccgagaatt
     1141 aatttcacag aagttcctac ttccgaccca attacaattg ccatggcatc gctaaagccg
     1201 actgcagagg catataataa actcaaagta atagcaactg ttgcggcaga agattgcgtg
     1261 atggttgttg ctatgaagcc caaaatcaga aaaacaaatg ctccactatc ccggaagcgc
     1321 gacatcgcct gaataatagc ctcatcctgt actgattgct gcataaaccc cagaccaatg
     1381 aataaaaagc tgaagcccat aaaaaatcgg gcaatcgacg aaaatttctt ttcagggaac
     1441 acaaatagca gcaatcccgc tactccggca acggggaggg catacatttc aatctcgagg
     1501 cgaaatccga tggtggcaac gatccagcta ttgagggtag tgcccagatt agtgcccata
     1561 atcacggcca gcgcattttt cagggagatt attccacttc ccgcaaatgc cagaaccaga
     1621 aaattgacca cagaactgct ttgtaatgcc gccgacaata ctgttccccc gaaaaccgct
     1681 ctgaaaggtc tgccggtttg cctcctcaaa aacagcttaa aacgccggct cataaggctt
     1741 ctaagcgcct tttcaagcag ttgtatcccg aacacaaaga ccccaattcc ccccagcaat
     1801 aaaccgatgt tcaatcccat aaatggctgt tacagggtta aacttagtta acaatgccgc
     1861 taaaaacaat gacaaggcgc attgccaaca gctttaaaca ggcttcaata gggcatatta
     1921 tatatcttta ccgccgctaa tcagcactta catgaagagt ttcaaaagta tcaacaacct
     1981 tacaggatgg atcgtatttg gcatcgcact ttttgtgtat gccagtacta tggaagctac
     2041 cgccagtttc tgggattgcg gagagtttat tgcagcaacg tataaactac aggtagtgca
     2101 cccgccggga gcgccaatct ttttgatgtt aggtaaaatt ttcagccttt ttgcctctac
     2161 gaaagaacaa gtgccggtaa tgacgaactt ctttgccgca ctttctacat cctttgccat
     2221 attattcact ttttggatca ttacaagact cataaaaaga attatgagcc caaatgatga
     2281 accaacattt tctcaaacta tagtcatcat cctcagcggt gttattggtg ctttggtttg
     2341 tttgtttatg gatagcatgt ggttctcagc tgttgaatcg gaagtttacg cattggcaac
     2401 atttttcttc gtgattattt tctggtcgat ggtaaaatgg gaagcccacg ccggtgatcc
     2461 taattctgat cgctggatat tattcatggc cttgcttatg ggattatcta ttggcgtaca
     2521 cttattagcc ttgcttgtgg ttcctgctat cgcattgata tattatttca gaaattataa
     2581 atacaccttc aaaggtttaa tgtatgcctt attgattggt gcaggattag tggcatttct
     2641 cctttatggt ctgctcgata aatttatcgg tctaggcgca tggatggatc gcgtgtttaa
     2701 taacttcgga gcaccattcg gcaccggtct cattatttac ggaattatga ttgttggcgg
     2761 tgtagtttgg ggtatacgca aagccataga aaaaagcaat cgcgtattat atctcgcttt
     2821 gatgtcattc actttcctta cgattggttt gacttcttat gcaatggtgt tgattcgcgc
     2881 tgaggcaaat cccgttatta atatgaacgg tattaatgat gtgcactcat ttctatcata
     2941 tcttaaacgc gaacaatacg gaagcaggga attgttatac ggacctttct ggacagcaca
     3001 acctgttgaa tacgaaaaag gaaaagctaa atggggtaag gttgaaggaa atgataaata
     3061 tcaaattatt tctcatgaat acaagccggt atataaaata ccgtcagagt atgccaacag
     3121 ccctgaggca caattgctga ttcaacgcaa ccgccagata ctattcccaa gaatgggatc
     3181 actggaggat cgtcatgccg gactttatta caatttcgcc aatgtgcctc aaggtcagga
     3241 agaaacgtac gttccgacag gtggagacaa tatgcggttt tttgctaatt atcagctcgg
     3301 acacatgttc tggcgatatt tcatgtggaa tttctcagga cgtcaaaatg attcacaagg
     3361 aaatttctac gatttatata aagatggaaa ctgggtaacc ggaatcacag cgattgataa
     3421 atcaaaaaat ccgcagttag tagatgtgcc tgcagaatta agtgatatcg attcgcataa
     3481 cgtatattat atgttgccgt ttatcattgg tatcattggt atggtgtatc aattgagaaa
     3541 tgacaaacga ggttttgcta ttgtattcat gttattcctc tttatgggat tcatgaatat
     3601 catcaacatg aaccagccac caacagaacc acgtgaacgc gactatgcac agattggtgc
     3661 attcttcgct tttgcactgt gggttggatt aggcctggct ggtattgtgg atatggctaa
     3721 gaattttgcc agcaaaaaag tattgcaaga atatgcacta tacagcttac tgattttatt
     3781 ggtgatgtat tttacaggac ttaccatgta taagttcact accatcgctt atgtaatgat
     3841 atatactgta ggctttatgt ttgttgtcgg ctttatcggc tcgctgattt ataaggcaac
     3901 aaaaaaagaa ctcactgtcg gcattgttgc attcctcatg cttgctcctg cgccatattt
     3961 aatggcaaca caaaactggg atgatcacga tcgcagcgat cgcacttttg ctcgtgatat
     4021 tgcgcgtaat tatctggaat catgccctcc gaatgccatc ttgtttacac aaggcgataa
     4081 tgatacttat ccattgtggt atgctcagga agtggaagga atcagaacag atattcgcat
     4141 cgtaaatctt agcttgcttg gtgtcgactg gtatatcaac caattgcgca atgcaacgaa
     4201 cgattctaaa ccgatagcat taacattagg acctgaaaag attttaggcg acaagcgaaa
     4261 tcaaatcgtt tccaacgata agagtaagtt caagaatacc actatggata ttaatgactt
     4321 cctcaacttt gttggtcagg atgatccgaa atatcagatt tacagtgaag gtttagaaga
     4381 ttatttcagc tacatgccta ctgataaatt aaaaatttcc atcgacaatg aagcaatgaa
     4441 atctcaaggt atcgttccgg aatcattaca gggcgatatg cgcaaagaga ttagcttcaa
     4501 actgacaaat aagacgctgt tgaaaaacga cttaatgaca ttagacattg ttgccaataa
     4561 tatcaacaag cgtccgattt gtttcgcggt gagtgttacc ggcgaatcac atcttggcat
     4621 gcaaaaatat ctgatgcaac gcaatatggt atatcaatta atgccggtgg aaatggaaaa
     4681 tgccaaaggt gttatggatg gtttccaaaa aggcattgag actgatatca tgtatgatca
     4741 attgatcaac cagaaagata aattcactta cggtggtgtt gagaaaggaa aacctattta
     4801 tatggatcca agtgcttacg gcagtatttt aactgctaag tatttgaatt atctcgacct
     4861 cgtaaaatcg ttgaatgatg aacgcgctta ccttgaagcg caggctcgtc agatgatgaa
     4921 agataccgtt aatcaggaat acactgaggt tggacaagaa ttgatgaagg agagtgccgc
     4981 taagaaagaa aaaatgatta cggttctcga ccagatgatg gtattattcc ctgattccag
     5041 tgttccttat gataacaata tggtgaatgt tgctcagctt tatcagcaat taggtaaacc
     5101 tgaaaaagca tctgaaatca ttaacacgat gatgccgcgc acattctcgg aacttgaata
     5161 ttattatcag atggctcgca acagcactgc aacggctcaa atgtatcagg atgatcagat
     5221 gcgttctgag caaacagtct acctgggtat agaaatactt aaaaaatctg atgcaaagga
     5281 tcaagctgac acttacgata aacagtggga agctcttcgc atgaaatatg gcatacccga
     5341 tttaaatgag agaaggagaa ggtaatttta aacgcaagaa gttcgacaat ctggttttcg
     5401 ggagacaacc ggttgtcgaa ctgcttatgc gtgaatcgcc ggtagacaaa attttattga
     5461 cgaaaactgc ccaaggcgat attataccgc agataaaaca tcttgccggc gaaaaacagg
     5521 ttcccgtgca gtacgttcct gttgaaaaat taaatcaact taccggtggc aatcatcagg
     5581 gtgtaattgc ctttactgcc gccgttgctt ttcaaaaaat cgatgatata cttccgttta
     5641 tcattgaaaa aggtgaagtg ccgctgattt taatattaga tggcattacc gatgtgcgca
     5701 attttggtgc tattgcccga acggcttatg ccggaggcgt acatgccatc attattcctg
     5761 cacatcatgc agcccccgtt aatagtgatg ccatgaaaac cagtgcaggc gcgctggcag
     5821 aaattcctat ttgtcgcgaa aagaattatg agttagtatt gaagtatctc aaactaaacg
     5881 gcttgcatat tatcggtgca gatgcacatg ccgataaatt tatctacaac agcgacctca
     5941 cggttcctac agcaattatc atgggttctg aagatgaggg tatcagcgca atttcacgca
     6001 agcatctcag cgaaacagta aaaattccca tgcaaaattc cttcgattct tataacgtat
     6061 ctgttgcagc aggtatgatc atttatgaag tgatgcgtca acgcgcgtaa aatcatactc
     6121 ccggcattat catttttttt aagcatcaac acataaatgc tgatgtctgt gccgtaatca
     6181 tagcctatac tctaagtatt tcgctatata ttcgattact atcttataag atcaggacaa
     6241 aaccagaccg ttttacatgg ccaatatctc aactaactga agcatctgct tgttaatctc
     6301 catatggtgc tttgtagcat tagcaaatgg cgtaaacaca acttcatcat tcacctgacc
     6361 tgccatcaca ttctttcttc cttcaatcaa agcttcaact gccgccatac caagtctgct
     6421 tccgagaatg cggtcgcgca cagtaggatt gcctccgcgc tggatatgtc ctaacacaga
     6481 aacacgagta tcgtaatgat taaatctttc cttcaccaat ttagcaatct caaatgcgcc
     6541 acctctctca tcaccttcgg ctacaataac aatggctgat gatttttgtc gcaaccagcc
     6601 tcgctccaaa gtagaaatga gataatcgat atttgtttcc gtttcaggaa ttacaattgc
     6661 ctctgcacca ccggcaatac ctgtactcaa ggcaataaat ccggcatcac gtcccatcac
     6721 ttcaacaaaa aacaacctgt tatgcgacat ggcagtatct cttattttgt cgatggcgtc
     6781 aattgcagta ttgatggctg tgtcaaatcc gagtgtataa tcggtgccat acaaatcatt
     6841 atcaatcgta ccggcaacac ctacacaaga aataccaaat tccttttcaa aaactgttgc
     6901 tccacggaaa gtaccatcac caccgatggc aacaaatcct tcaatgccat tgagctttaa
     6961 attatcgaag gccatttttc gtccttctgc agtcataaat ctttcgcttc gcgccgtttt
     7021 taggatggtt cctcctcgtt gaatgatatt gctgac
//
LOCUS       DAOQOU010000167         7054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6028_length_7054_cov_6.022860, whole genome shotgun sequence.
ACCESSION   DAOQOU010000167 DAOQOU010000000
VERSION     DAOQOU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6028_length_7054_cov_6.022860"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..1314
                     /locus_tag="PLM90_12140"
     CDS             553..1314
                     /locus_tag="PLM90_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90176.1"
                     /translation="MKHCLVAIFVVLAGVVSAQTTAENWTQTDCDGVDHDLFTTLDTG
                     AAVVMEFVMLESCMPCINAAHMMAPIIDRYDLLYNNRVQYYTFGYNDTYLCDDFATWE
                     SDNAIANDAALLLGADISSYYGGMGMPTIVVVGRNTHQVYFNQFGFVIGDTTDFSNAI
                     AYALGIADPETGVECMDDEQLLISPNPAFNSIQINGILEPNATLRIINLQGQICMDGI
                     NPTENPIDIQMLKPGMYLLECKTDTGISIAKFTVQ"
     gene            complement(1317..2171)
                     /locus_tag="PLM90_12145"
     CDS             complement(1317..2171)
                     /locus_tag="PLM90_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ90177.1"
                     /translation="MKHILIPSDLRIESDPVWQYVLPLVKTMGASLHFVHAVDDPFVK
                     DERDHVGAEGVDKITSDLVYKYRDEADKAMIKLLDRVKAAMTGMDIHVEGHVRNGNAE
                     EVILHEAAEIQPQLIVMGSHDHSKLDRMFFGSVTQAIIRRSHFPVLAIPEKYIFKPVN
                     QIVYMSDLDNNDTISIAKLLNLFGGNAFNLHITHFNLDEVSKDEALYAIGDKIRNDHK
                     EIQIDFEVVDARVLRDAYKQYISMKNIDLIALTTRKDHGMMRFINPGTAIDVLYHSKL
                     PVLIFHRA"
     gene            2846..3745
                     /locus_tag="PLM90_12150"
     CDS             2846..3745
                     /locus_tag="PLM90_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMZ90178.1"
                     /translation="MSAIYSIRDLESLSGIKAHTIRIWEQRYGILKAARTETNIRYYS
                     ETELKYLMSLALLNRNGFKISKLAIYSPDEIAEKVEHLINEPQEFHPQIEALTLATMA
                     YDEEKFDKVLNTTILQMGLEAAMRKVIFPYLDKLGMLWVSGSVIAAQEHFMTQLLRQK
                     LIVAIDGQIPHYTGDSLTYVLFLPNGEWHELGLLFLHYLLKSRNHRVIYLGPSVPLQD
                     VLQVGESLNPNAFYTVLTKNPNGFHVVDYLNKLAEAFPCSTVFSSGSQVAGPLKGLAS
                     NVKIIDSIDSIVDSPEVLMAENR"
     gene            3832..4350
                     /locus_tag="PLM90_12155"
     CDS             3832..4350
                     /locus_tag="PLM90_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ90179.1"
                     /translation="MTSSEFQQEIVQLKAPMKAFALNLTRDRDDALDLVQETYMRALS
                     NQEKFHDGTNLKAWLLTIMKNIFINNYRKASKRNTVADASDNLYLLNAGVATAGNEAE
                     KDFVMKDLLKAIQQLDAEYKRPFIMHYHGYKYEEIADAMKLPLGTVKSRIFFARKQMK
                     DFLDKRGFERFQ"
     gene            complement(4392..4778)
                     /locus_tag="PLM90_12160"
     CDS             complement(4392..4778)
                     /locus_tag="PLM90_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ90180.1"
                     /translation="MITGVHHIAIICSDYHRSKKFYTEVLGFSIIAEHYRAERDSYKL
                     DLRVDENTAIELFSFPGPPARPSYPEAAGLRHVCFKVKDLDAAIARLQGFQVECEDVR
                     IDAYTGMRFTFFADPDGLPIELYEDK"
     gene            complement(4775..5137)
                     /locus_tag="PLM90_12165"
     CDS             complement(4775..5137)
                     /locus_tag="PLM90_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HMZ90181.1"
                     /translation="MRWEQSLGKEGEDRAAVLLQNKGYTILRRNYRFGRAEVDIIARK
                     GNILVIVEVKTRSGDPDIDPGDSITRKKINLLAGAAEAFMLDEGLMLLLRYDAILVEK
                     QGIHWEITHIEDAFYPAE"
     gene            5163..6143
                     /locus_tag="PLM90_12170"
     CDS             5163..6143
                     /locus_tag="PLM90_12170"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HMZ90182.1"
                     /translation="MDKYIQQFPEQLVDALRIVDSTATFQLPRTPKHVITSGMGGSGI
                     GSVIVRTLISQQLTVPFAFSSDYRLPGWTNSDTLVLCSSYSGNTEETLSAMQFALDRG
                     CMVVCVTSGGKMLEIAKEKNLPVVIIPGGRPPRSSIGYSIIAQLFLLQHCGIIDNSWH
                     ASMEKCIAFIHENMSVIREQAESLAVEIDGSIPVIYSDALLAGTAVRWKQQLNENAKI
                     HAFTHVVPEMNHNELVAYYHPDHRINVICLRHAPENPRTAKRFSLMEELIQGKAGSII
                     TVLATGPTLFEQIFGLIHLGDWLSVALAERKGVNPLDIEMLEWLKKALDK"
     gene            6143..6844
                     /gene="lipB"
                     /locus_tag="PLM90_12175"
     CDS             6143..6844
                     /gene="lipB"
                     /locus_tag="PLM90_12175"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HMZ90183.1"
                     /translation="MMQLVHFRDLGTMDYAKAWQLQEGVFRDIIDKKLQNRLLPIEKQ
                     LSYQHQLLFVEHPPVITLGKNADDNNVLFNEEALAQRGVQLFHINRGGDVTYHGPGQL
                     VGYPILDLDMFTTDLGVYLRNIEEVIIRTIADYGLVGERSPGETGVWLDVGKPTARKI
                     CAIGIRTSRWVTMHGFAFNVNANLDHFNFIIPCGIPDKAVTSLQNELNSMMSMDEVKQ
                     KVRHYFEIVFDCKLV"
     gene            6844..>7054
                     /locus_tag="PLM90_12180"
     CDS             6844..>7054
                     /locus_tag="PLM90_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HMZ90184.1"
                     /translation="MAEQDDELELESIAQEGEEELYEHYRVTTDRGQEPLRIDKFLFN
                     RLINISRNRIQNAADAGCILVHGKPV"
BASE COUNT         2013 a         1554 c         1454 g         2033 t
ORIGIN      
        1 ggtctacacg aggccgaata attatgggtt ttgagtgggg gcagtgggtg agtggtgagt
       61 ggtgagtggt gagtggtgag tggtgagtac ttgatacctg ctactagatt attgcgcctt
      121 agcctcttag cgccttcgca cgaaaccgcc tcacccccgg ccactctcca tctcctatcg
      181 tcgatagaga gggagctaat tacgcttcgg aattaaaata ttgcaaagca atataattgc
      241 attccctatt aacgcgccag cgttaatagc ccctcacact tgctaatttg ctaatttgct
      301 aattctcgcc tcaccccccg ccctctccat ctcctatcgt cgatagagag ggagctaaat
      361 tcgcttcgga ataaaatttt attaaacaat gtaaaacgca ttccctatta acgcgccagc
      421 gttaaaagcc cataacactt gctaacttgc taatttgcta atttgctaat taaccctcct
      481 tttcattcga atagcattta ttcaatcccc tgagaaaaga cccaatcacg aactatttcc
      541 tatctttatc tcatgaaaca ttgtcttgta gcaatttttg tggttttggc cggtgttgtt
      601 tcggcgcaga caaccgctga aaactggaca cagactgatt gcgacggtgt tgaccatgac
      661 ctgtttacta cactcgatac cggtgcggca gtggtgatgg aatttgtgat gcttgagagt
      721 tgtatgccct gcatcaatgc tgcacatatg atggcgccga ttattgatag atacgattta
      781 ctttacaaca accgagtaca atattatacc ttcggatata acgacaccta tttgtgcgac
      841 gattttgcaa catgggaaag tgataatgct attgcaaatg atgcagctct cttattgggt
      901 gccgatatca gcagttatta cggcggaatg ggaatgccca ccatagtcgt ggtgggaaga
      961 aatacccatc aagtttattt taatcagttc ggatttgtga tcggcgatac tactgatttt
     1021 tctaatgcta tagcgtatgc actcgggata gcagatcctg aaacaggcgt tgaatgtatg
     1081 gatgatgagc aattattgat atcacctaat cctgctttta atagcattca aataaatgga
     1141 attcttgagc ctaatgcaac attgcgcata ataaatctgc aaggtcaaat ttgcatggat
     1201 ggaattaatc cgactgaaaa tcccatcgac attcaaatgc ttaagcccgg catgtatctt
     1261 cttgaatgca aaacggacac aggcatttct atagcgaagt ttacagtgca ataaatttaa
     1321 gctctgtgaa atatcaacac aggcaatttg ctgtgatata atacatcaat agcagttccc
     1381 ggatttataa atcgcatcat accatgatct ttgcgtgttg taagggcgat tagatcgata
     1441 tttttcatgg atatatattg cttataagca tcgcgcaata ccctcgcatc taccacttca
     1501 aaatctatct gtatttcttt gtgatcattg cggatcttgt cgccaatggc atataatgct
     1561 tcgtctttgg atacttcatc aagattgaaa tgggtgatat gcaggttgaa tgcatttccg
     1621 ccgaataaat tgagtaactt ggctatagat atagtatcgt tattgtctag atcgctcatg
     1681 tagactatct gatttaccgg cttgaatata tatttctcag gaatagcaag taccggaaaa
     1741 tgtgagcgac gaataattgc ctgtgtcaca cttccaaaaa acatgcgatc cagtttgctg
     1801 tggtcgtgac ttcccattac aatgagttga ggttgtattt ccgcagcttc atgcagtatc
     1861 acctcttcgg cattaccatt gcgcacatga ccttcaacgt gaatgtccat tcctgtcatg
     1921 gcagccttaa cgcggtccaa taactttatc atcgccttat ctgcttcatc gcgatattta
     1981 tataccaagt cgctggtaat tttatcaaca ccctcagctc caacatgatc gcgttcatct
     2041 tttacaaatg gatcgtcaac agcatgcaca aaatgaaggg aggcacccat ggttttgacc
     2101 agtggtaata catattgcca aacaggatcg gattcaatgc gcaagtcgct cggaatcagt
     2161 atatgtttca tattagtggt tttgatggta aagataaagg caggtcattt caaaaagaat
     2221 gaaataagtc accattctta atcatcccgt aaaccttcat ctgactatca cgcctctgct
     2281 atctagattt cagtctgcaa ccgactggag gtattagtat ctagaattaa gtatctagag
     2341 ccaagctcgc ctcgcctccg gcgggtatct agtatcaaga cttggtatca ggtatcaagt
     2401 atcaggtatc aagtatcagg tatcaagtat caggtatcag gtatcaagta tcaggtatca
     2461 ggtatcaagt atcaggtatc aggataaact caccactcac gagtcacgat tcacgagtca
     2521 cgattggtag tgggtcagtg ggtgagtggg gcagtgggtg aatggtgagt ggtgagtggt
     2581 gagtttttga ctgcttctcc cttcatttct tccttcaaat cttcggttct tcgtccttcg
     2641 aatcttcggt tcttcttcct tcgaatcttc gattcttcgt ccttcgaatc tttggttctt
     2701 cgtccttcga ttctttccac cttcaaatct tcgaatcttc aaatcttcga ttcttccttc
     2761 aaccttcaac cccacctcaa tgaacctata gtctccataa ttgtttaacc attgaaatac
     2821 aaaacttaaa cattaatttt acctcatgtc ggccatctat tccatacgcg atctggagtc
     2881 gctttcgggt ataaaagcac ataccatcag gatttgggag cagcgttatg gcatattgaa
     2941 agcggctcgt accgaaacga atattcgcta ttacagcgaa acggagctaa agtatctgat
     3001 gagcctggct ttgctcaaca ggaatggatt caagatatct aaactggcca tttattcgcc
     3061 tgatgagatt gccgaaaaag tagaacacct catcaatgag ccacaggaat tccatcctca
     3121 gatagaagcg cttacgcttg ctaccatggc ttatgatgaa gagaaatttg ataaagttct
     3181 caataccacc atcctgcaaa tgggtcttga agctgccatg cgtaaggtga ttttccctta
     3241 tctcgataaa ctgggcatgc tctgggtttc tggctccgta attgcggcgc aggaacactt
     3301 tatgacgcaa ttactgcgac aaaaactgat tgtcgccatc gatggacaaa taccgcatta
     3361 taccggcgat tcacttacct atgtgctgtt tctcccgaac ggagaatggc atgaattggg
     3421 attgcttttc ttgcattatc tgctcaaatc aagaaaccat cgggtgatat atctcggacc
     3481 cagcgtaccc ctccaggatg ttctgcaggt tggcgagtcg ctgaacccca atgcctttta
     3541 taccgtcctc acaaaaaacc ctaacggctt tcatgtcgta gattatctga ataagctggc
     3601 tgaagcattt ccttgcagca ccgtattttc aagtggctct caggttgcgg gacccttgaa
     3661 aggattggct tccaacgtta aaatcatcga ttctatagat agtatcgttg atagtcccga
     3721 agtgctgatg gcggaaaatc gttaatattt tgttgaagat ttaacatcaa aagttaaaca
     3781 aaaatttgga aacggacttt ttgagccgta aatttgcttc aaacaacgac catgacaagc
     3841 tcagaatttc aacaagagat cgttcagctt aaagcgccaa tgaaggcctt tgccctgaac
     3901 ctgacccgcg atagagatga tgcgctcgat cttgttcagg aaacttacat gcgtgcgctc
     3961 tcgaatcaag agaaattcca tgatggaacc aacctcaaag cctggttgct cactatcatg
     4021 aaaaatatct tcatcaacaa ttaccgcaaa gcatcgaaaa ggaacactgt tgccgatgcc
     4081 agcgataatc tttatctgtt aaatgcaggt gtggccactg caggtaatga agcagagaaa
     4141 gattttgtga tgaaggatct actcaaggct attcaacaat tagatgccga atataagcgc
     4201 ccattcatca tgcattatca tggatataaa tacgaagaaa ttgcagatgc catgaaattg
     4261 cctttaggca ccgtgaaaag ccgcatcttc ttcgcaagaa aacaaatgaa agattttctg
     4321 gataaacgcg ggtttgaaag atttcaataa atcgatcatg cgcttaataa ttgattgagt
     4381 tttctattca atcatttgtc ttcatataat tcaataggca atccatcagg atcggcgaaa
     4441 aatgtaaatc gcatgcctgt atatgcatca atacgcacat cttcacattc cacctgaaag
     4501 ccttgcagac gcgctattgc agcgtcaaga tcttttactt taaaacatac atgtctcagt
     4561 ccggcagctt ccggatacga cggacgagca ggcggacccg gaaatgaaaa taattcgata
     4621 gccgtatttt catctaccct caaatccaac ttataactat cgcgttcagc acgataatgt
     4681 tcggctataa tcgaaaatcc caatacttca gtatagaatt ttttcgacct atgatagtcg
     4741 gaacaaataa tggcaatatg atgtactcct gttatcattc cgcaggataa aatgcgtcct
     4801 caatatgtgt gatctcccaa tgtatgcctt gcttctcaac taatatggca tcgtaacgca
     4861 gtaatagcat taatccctca tctaacataa aagcttctgc agccccggca agcaaattta
     4921 ttttttttct ggtgatgcta tctcccggat ctatatcagg atcaccgctt cgtgttttca
     4981 cttccacgat aaccaggata ttcccctttc tggcaataat atcgacctcc gccctgccaa
     5041 agcgataatt cctgcgcaaa atggtataac ccttattttg caataaaacg gcggctctat
     5101 cttcaccttc ctttcccagc gactgctccc atctcataat accgtatttt tgtcggctta
     5161 ttatggacaa atatatccag caatttccgg aacaactggt agatgccttg cgtattgtag
     5221 acagcacggc tacttttcaa ttaccaagaa ccccaaaaca tgtgattacc tccggtatgg
     5281 gtggttcggg tataggctcg gtaattgtgc gaacgcttat ttcgcaacag cttactgtgc
     5341 cgttcgcttt cagctccgat tacaggctcc cgggctggac aaacagcgat accctcgtgc
     5401 tctgtagcag ctattccggc aataccgaag aaaccctcag cgccatgcaa tttgccctcg
     5461 atcgtggctg tatggttgta tgtgttactt caggaggaaa aatgcttgaa attgctaagg
     5521 agaaaaatct cccggtggta atcattcccg gtggtcgacc gccgcgctcg tcgataggct
     5581 acagcatcat cgcgcaatta tttctgttgc aacactgcgg aataattgat aatagctggc
     5641 atgcgtcaat ggaaaaatgt atcgcattca tacatgaaaa catgtctgtt attcgcgagc
     5701 aagctgaatc actggcagtg gagatcgatg gcagtattcc ggtaatttac agcgatgcat
     5761 tattggcagg cacagctgtg cgttggaaac agcaactcaa tgaaaatgca aagatccatg
     5821 catttaccca tgtagtgccg gaaatgaatc acaatgaact cgttgcctat tatcatcccg
     5881 atcatcgcat aaatgtgatt tgcctgcgtc atgctcctga aaatccacgc accgcaaagc
     5941 gattttcact gatggaagaa ctcattcagg gaaaagcggg ttctattatc accgtgcttg
     6001 ccacaggtcc aacattattt gaacaaatat tcggacttat acatctcggc gattggctgt
     6061 cggttgcact cgctgaacgc aaaggagtga accctttaga tattgaaatg ttggaatggc
     6121 tgaaaaaggc attggataaa taatgatgca gctagtacat tttcgcgatc tcggtaccat
     6181 ggattatgca aaagcgtggc aattgcagga aggtgttttt cgtgatatca ttgataagaa
     6241 attacaaaac agattacttc cgatagaaaa acaattatcg tatcaacatc aattattatt
     6301 tgttgaacat ccacctgtta tcacactagg gaaaaatgcc gatgataata atgtattatt
     6361 taacgaagaa gcgctcgcac aacgcggtgt tcaattattc catataaatc gcggtggcga
     6421 tgtaacgtat catggtcccg gacaattagt aggttatccc atcctcgacc tggatatgtt
     6481 tacaactgat cttggcgtgt atttacgcaa tattgaagaa gtgatcattc gcaccattgc
     6541 agattacgga ttagttggtg aacgcagtcc cggagaaacc ggcgtatggc tggacgtagg
     6601 aaaacctaca gccagaaaaa tttgcgctat cggcatcaga acgagcagat gggtaaccat
     6661 gcatggattt gcttttaatg tgaacgccaa cctcgatcat tttaatttca tcatcccctg
     6721 tggtattccc gacaaagcgg taacatcact gcaaaatgaa ttaaatagca tgatgtcaat
     6781 ggatgaggtg aagcaaaaag ttcgtcatta ttttgaaatt gtttttgatt gtaaattagt
     6841 gtgatggcag agcaggacga cgaactcgaa ttagaaagca tcgctcagga aggcgaggag
     6901 gagttatacg aacattatcg tgtaaccacc gatcgtggac aggagcctct gcgcattgat
     6961 aaatttttat tcaaccggct cattaacatc tcccgtaacc gcattcaaaa tgccgctgat
     7021 gcaggatgta ttctcgtgca tggaaaaccg gtta
//
LOCUS       DAOQOU010000168         7012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6081_length_7012_cov_6.047434, whole genome shotgun sequence.
ACCESSION   DAOQOU010000168 DAOQOU010000000
VERSION     DAOQOU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6081_length_7012_cov_6.047434"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..590
                     /locus_tag="PLM90_12185"
     CDS             228..590
                     /locus_tag="PLM90_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ90185.1"
                     /translation="MHNYKSLIVWQNAMELVKEVYLTTKTFPKEEIYGLVQQIRRSAI
                     SIPSNITEGCGRKSDKDFDNFLSIAHASSFELETQLMIAESLSLIKNEESQLLLSKTS
                     LVQKKLFKLKESLKLKNN"
     gene            668..2452
                     /locus_tag="PLM90_12190"
     CDS             668..2452
                     /locus_tag="PLM90_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ90186.1"
                     /translation="MSSNNLILKGGSFLLHQSTPSDIFIPEELNEEQLMIKQMTTDFV
                     QKEVLPNLDRIDKHEEGLMAALLTKAGEIGILGLPIPEAYGGFAKDFNTISAVTEVTG
                     TAHAFSVAYGAQSGIGTLPILYFGTEEQKKKYLPGLAAGTTHASYCLTEPGSGSDALA
                     AKTKAVLSEDGKHYILNGQKMWITNAGFADIFTVFAKIDGDKFTAFIVERGAPGFTIG
                     VEEEKMGIKGSSTCQIFFENTPVPVENVLGEIGKGHLIAFNILNIGRYKLCVGVLGGA
                     KALIDTSVKYANERMQFNVPIASFGAIKYKLSEQAIRTFAALSATYRTSDYIDKKEKE
                     LLSKGIGHEKALLGAAEEYAIECAMLKVIGSEVLDFVVDECVQIHGGNGYSEEYPAAR
                     AYRDARINRIFEGTNEINRLLTVDMLLKRVMKGQLDMLGPAMAVQKELMGIPELGETG
                     EGLFYEEEKAIENMKKAFFMVAGAAVQKLMMALKDEQEILMCAADMMNEIFTAESMLL
                     RIKKLTLQNGEDATSIHRDMLAVYFTDSIDRLNKQGRTAIASFASGDELRMMLLGMKR
                     FTKYRTVNTTAARRRIADKMIAENGYCF"
     gene            complement(2462..3712)
                     /locus_tag="PLM90_12195"
     CDS             complement(2462..3712)
                     /locus_tag="PLM90_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90187.1"
                     /translation="MRITLLLFGLLSCIAASAQSRLTMNDNPYIVMNGSVQMVIDNNN
                     ANAITTTGSGGNIISESETNIISWHINTATGSYIVPFTTSDGVKIPLTVQITSAGAGA
                     GVIDFSTYGGLTWNNDTYKPGEVTNMTNMGVTNNSTEIIDRFWIIDAHGYTTKPSGNI
                     QFNYDDAEHLAAGNTINEPDLKAERYEPGTNNWEVFPVGGIINTGSNYVTGVPFNSGD
                     FMRTWTLIDQTTHLLPIVLTNFSATCVAGGTNLQWTTASEINSDHFIIAGSTDGIYFE
                     DLADIQAAGNSNTPTSYGYTADDNMFRYYKLSLLNTDATIEELGILSTSCNQQIAPEF
                     FAYSPASFQITAQTQQLTPGNYVISIFTLQGQLITGSNLQLGTEVATMTYYDQSLSDG
                     IYIVALRSTDNPKITMTSKVALFH"
     gene            complement(3714..6371)
                     /locus_tag="PLM90_12200"
     CDS             complement(3714..6371)
                     /locus_tag="PLM90_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HMZ90188.1"
                     /translation="MKRIILLLLTFWVLGTQAQNIAINTDGSTADNSAMLEIKSSTKG
                     FLVPRMTAAQKTAIPTPATGLLIYQTDGSTGFWYYNGAAWTQISGSAGWSLTGNAGTV
                     AGTNFIGTTDDVALQFKIDNTVAGGINKNGNTSLGYKSLQNTTTGTSNIAIGKMTLNA
                     NTTASGIVAIGDSSLSKNGTGAAFLWEGANNTSVGNRSSKNNTTGYDNTAIGSYSLQN
                     NTTGANNTALGSSALYYTTTGNNNLAAGSYSLLFNTTGAYNVATGQSGMVSNTTGSYN
                     TASGFASLFNNTTANYNVANGTYSGYYNTTGNRNTFVGYGAGYFNTTGYLNTCIGSQA
                     GYSTTTAFSNVFVGEQAGYSNTTGYSGVYIGQDAGYFNTTGVENTFIGTDAGEANTTA
                     SYNTFVGTNSGLLNTTGYQNTFVGQDAGRANTTGMWNTFLGEDAGYANTTGNFNTLVG
                     VNAGQANTTGSKNIFIGVGAGHDNTTASNNIVIGTDAALSGTTGSNNVFIGTESAYTN
                     TTGFDNTYVGNYGGRSNTTGYYNVGVGKNVLYANSTGIWNTASGAFSMMDNTTGSYNT
                     AFGINALRYQTTANYNVALGATSMQQTTTGNQNLSAGYAALLTNTTGSSNVALGYQSL
                     YFNTTASWNTATGYMTMYNNTTGSVNVASGYSALYSNTTGTNNVALGSSALIGNTTGA
                     RNVGVGSGAGDLNDNNTNCTFLGYDADQAVTTDFDNSTALGNGSRITASNQVRVGNAS
                     VTSIGGYVGWTNISDGRVKKDVTENVPGLAFIMALKPVTYHLDIDAINDHLHVADSVQ
                     NLQSIAAKNKVLYSGFIAQDVAAAAKQIGYDFSGVDAPKSEDDLYGLRYADFVVPMVE
                     AMQEQQKLIEKQQEQIDALTKLVQSMQNH"
     gene            complement(6529..>7012)
                     /locus_tag="PLM90_12205"
     CDS             complement(6529..>7012)
                     /locus_tag="PLM90_12205"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90189.1"
                     /translation="PGYYFDAPIDTTGTVYVKFDARNYHDGAFNPIPGCGTVEVHFFS
                     VLDSADNNAVALFHARLGVSEEDCGVDVVSSVLDNSFAIYPNPAVNNINVIASFSANI
                     AKVDIVNIVGRTVRTYDWNTGSGKMSFDISDLPQGVYFARLIDEAGKVVYAEKVSVLK
                     "
BASE COUNT         2048 a         1401 c         1444 g         2119 t
ORIGIN      
        1 gtatcaggta tcaggtatca ggtatcaggt atcaggtatc aggtatcaag tatcaagtat
       61 caggtatcag gtatcaggta tcaggtatca ggtatcaggt atcaagtatc aggtatcagg
      121 tatcaggtaa taatgggatg gtaaatttaa agaggggatt tatatgtata tattagagtt
      181 tagaagagat taattaaaat attttgttta acccttaata attatttatg cataactaca
      241 aatcactaat tgtgtggcag aatgccatgg agcttgtaaa ggaagtttat ttaacaacta
      301 aaacattccc taaggaggaa atatatggac tggtgcaaca aataagaagg tcggccattt
      361 ctattccttc aaatattaca gaagggtgtg gaagaaaatc agataaagac tttgataatt
      421 tcttgtcaat tgcacatgcg tcctcatttg aactagaaac gcaattaatg atcgctgaaa
      481 gtttaagcct tattaaaaat gaggaatctc aattactatt atcaaagaca tcattagtcc
      541 aaaaaaaatt gtttaaactc aaagaatcac tcaaacttaa aaataactaa ataatctcga
      601 atcatctttg gcaaatacca atacctgata cttgatacct gatacttgat accaaaaaat
      661 taattatatg tcatcaaata atctcatact aaaaggaggc tcattcctcc tccaccaatc
      721 caccccgtca gacattttta ttcctgagga attgaatgaa gagcagctta tgatcaagca
      781 gatgaccacc gactttgtgc agaaagaggt gctgccaaat ttggatcgca tcgacaagca
      841 tgaggaaggc ttaatggccg cattgcttac taaagccggt gagattggaa tactgggttt
      901 gccaattccc gaggcgtatg gtggatttgc aaaagatttt aatactatct ctgctgtgac
      961 ggaagtaaca ggtactgcac atgctttctc agttgcttac ggggcacaaa gtggcatcgg
     1021 tactttgccg atcttgtatt tcggtacaga ggaacagaag aaaaaatacc tgccgggact
     1081 ggctgccgga actacgcatg caagctactg tctcacagaa cctggaagtg gtagtgatgc
     1141 gcttgctgca aaaacaaagg cagtgctgag tgaagacggc aaacattata tcctcaacgg
     1201 gcaaaagatg tggatcacca atgcaggatt cgcggatata tttacggtgt tcgcaaaaat
     1261 tgatggtgat aaattcactg ccttcattgt tgagcgcggt gcccccggat ttaccattgg
     1321 tgtagaagaa gaaaaaatgg gtattaaggg ttcttcaaca tgtcagattt tctttgagaa
     1381 tacgcctgtg cccgttgaaa atgtgttggg ggaaattggg aagggacatc tcattgcatt
     1441 taatatcctc aacataggca gatacaaatt atgtgttggc gtacttggtg gtgcgaaggc
     1501 gctcattgat acatcagtta aatatgcaaa tgaacgtatg caatttaatg tgcctatcgc
     1561 ttcgtttgga gcaataaaat ataaattgag tgagcaggct attcgcacct tcgctgcatt
     1621 aagtgctacc tatcgcacca gcgattacat cgacaaaaaa gaaaaggaat tattgtcgaa
     1681 aggtatcggc cacgaaaaag cactactagg tgcagcagaa gagtatgcta ttgaatgcgc
     1741 catgttgaag gtaataggta gtgaagtgct cgattttgta gtagatgagt gtgtgcaaat
     1801 ccatggtggg aatggctata gcgaagaata tcccgctgca cgcgcatatc gcgatgcaag
     1861 gattaacaga atattcgaag ggacgaatga aatcaataga ctattaacgg tcgacatgtt
     1921 gctcaagcgc gtcatgaaag ggcaattgga tatgcttggt cccgcaatgg cagtgcaaaa
     1981 agaattgatg ggtattccgg aattgggaga aacaggtgaa ggtctgttct atgaagaaga
     2041 gaaagctata gaaaatatga aaaaggcatt cttcatggtt gcaggggcag cagtgcaaaa
     2101 attgatgatg gcgttgaaag atgaacagga aatattaatg tgtgctgccg atatgatgaa
     2161 tgagattttt actgctgaat ctatgttgtt gcgcattaag aaactcaccc ttcagaatgg
     2221 tgaggatgcc acgtcaatac accgcgatat gcttgctgta tattttacgg atagcataga
     2281 tcgtctcaat aaacaaggca gaaccgccat tgcatctttt gcttccggcg atgaactgcg
     2341 catgatgtta ctcgggatga aacgctttac gaaatatcgc acagtgaaca ccactgcagc
     2401 acgcagaaga attgcagata aaatgattgc cgaaaatgga tattgtttct aatcgaaaat
     2461 tttaatgaaa taaggccact tttgaagtca ttgtgatttt tggattgtcg gttgacctga
     2521 gtgctacaat ataaatacca tcagataaac tttggtcgta atatgtcatg gtggcgactt
     2581 ctgtacctaa ctgtaaattg ctgccggtta ttaattgccc ttgcaatgta aatatcgata
     2641 ttacataatt tccgggtgtc aactgctgag tttgtgcagt aatctggaaa gaagctggtg
     2701 agtaggcaaa aaattccggt gcaatttgct ggttgcagct agtgctaaga atgcctagct
     2761 cttcaattgt tgcatccgta ttcagcaaag aaagtttata ataccggaac atattgtcgt
     2821 cggcagtgta accataggat gttggtgtat tactgtttcc cgcagcttgt atgtcggcaa
     2881 ggtcttcaaa gtaaatgcca tcagtgcttc ccgctattat aaaatgatct gagtttattt
     2941 cagaagctgt agtccattgt aaatttgtac ctcctgctac acaggttgcc gagaaatttg
     3001 taagcacaat tggaagtaaa tgtgttgttt gatcaattag cgtccatgtg cgcataaaat
     3061 cgccggaatt aaatggaaca cctgtaacat aattgcttcc tgtgtttatt attcctccaa
     3121 caggaaatac ttcccagtta tttgtaccgg gttcatatcg ctcagctttt aaatccggtt
     3181 cattgatagt atttccagca gcaagatgtt ctgcatcatc ataattgaat tgaatatttc
     3241 ctgaaggctt tgtagtgtat ccatgtgcat ctatgatcca aaatcgatct ataatctctg
     3301 tcgagttgtt ggttacaccc atattggtca tattggttac ttctcccggt ttataggtgt
     3361 cattattcca tgtcaggccg ccataagttg aaaaatcaat tactcctgct cctgcacctg
     3421 cacttgtaat ttgcactgtt aatggaattt ttacaccatc gcttgttgtg aatggtacta
     3481 tataactgcc ggttgccgta ttaatatgcc agctgataat atttgtttct gattcactga
     3541 taatattccc gccactccct gttgttgtaa tcgcgttggc attgttatta tcaattacca
     3601 tttgcacgct gccattcatt acaatatatg gattgtcatt catggtcaac cgcgattgcg
     3661 ccgacgctgc tatacatgat agcaaaccaa ataataataa ggtgattcgc atatcagtga
     3721 ttttgcatgg attgaaccaa tttcgttaat gcatcaattt gctcctgttg cttctcgatg
     3781 agcttttgct gctcctgcat tgcctctacc atcggcacaa cgaagtcggc atagcgcaaa
     3841 ccataaagat catcctccga tttaggagca tcaactccac tgaaatcata acctatttgc
     3901 ttagcagcag cagcaacatc ctgtgcaata aatcctgaat acaaaacctt gttttttgcg
     3961 gcgatagatt gaagattttg aacagaatct gcaacatgta aatggtcgtt aatggcatca
     4021 atatctagat gataggtaac aggtttaagt gccatgatga atgctaaccc cggtacattt
     4081 tctgtgacat cttttttcac tcgaccatca gaaatatttg tccaaccaac ataaccgcca
     4141 attgaagtca ccgaggcgtt gcctacacgc acctgattgc ttgcagtaat ccttgagcca
     4201 ttgccgagtg cagttgaatt atcaaaatct gttgttacag cctgatccgc atcataccct
     4261 aaaaaggtgc agtttgtatt attgtcattg agatctcctg ctcctgagcc aacaccaacg
     4321 tttcgcgctc ctgttgtgtt acctatcagc gcgcttgagc ccaaggctac gttgtttgtt
     4381 cctgtagtat tactatataa tgcactatat ccggaagcaa cattcacact gcctgttgta
     4441 ttattataca tggtcatata acccgtggct gtattccatg atgctgtagt attgaaatat
     4501 aatgactggt aacctaaggc cacatttgaa cttcctgttg tatttgtcaa taaagcagca
     4561 taacctgccg ataggttttg atttcctgtt gttgtttgtt gcattgatgt agcacctaac
     4621 gcaacattat aatttgccgt tgtttgatat ctgagtgcgt taataccaaa agcagtatta
     4681 taacttcccg tagtattatc catcattgaa aatgcacctg atgccgtatt ccaaattccg
     4741 gtagagttgg catacagcac attttttcca actcctacat tataataacc ggttgtattg
     4801 cttctaccac cataatttcc tacataggta ttatcaaaac cggttgtatt cgtatacgcc
     4861 gactcagttc caataaatac attgttgctt ccggttgtgc ctgataatgc agcatcagtt
     4921 cctataacaa tattattgct tgcagtagtg ttgtcgtgtc ctgctccaac gccgataaaa
     4981 atgtttttcg aaccggtagt atttgcttgt ccggcattta caccaaccag tgtgttgaaa
     5041 tttcctgtag tgtttgcata tccggcatct tcacccagaa atgtattcca catacctgtt
     5101 gtattcgccc ttcctgcatc ctgaccaaca aatgtattct gataacctgt tgtgttgagc
     5161 aaaccggaat tcgtgccgac gaaggtgttg taacttgctg ttgtatttgc ttcgccggca
     5221 tcggttccga taaatgtatt ttctacgcct gtagtattaa aatatcctgc atcctgtcct
     5281 atataaaccc cgctataacc tgtagtattt gaataaccgg cttgctcacc tacaaataca
     5341 tttgaaaatg cagttgttgt tgaatagcct gcttgtgatc caatacaagt atttagatat
     5401 cctgtagtat taaaataacc tgcgccataa ccaacaaaag tgtttctgtt gccggtggta
     5461 ttgtaatatc ctgaatatgt gccgttagct acattataat tcgctgttgt attattaaat
     5521 aatgatgcaa atccggaggc ggtattatat gaaccagtgg tattcgaaac cataccgctt
     5581 tgacctgttg caacattata tgcgccggta gtattaaaaa gcaagctata tgaacctgca
     5641 gcaagattat tattacctgt tgttgtgtag tataaggcgc ttgagcctaa agcagtgttg
     5701 tttgctccag ttgtattatt ctgaagcgaa taacttccaa tggccgtatt atcataaccg
     5761 gttgtgttgt ttttcgaaga cctgttacct acactggtat tgttagctcc ctcccataaa
     5821 aatgctgcac cggtaccgtt tttcgatagt gaagagtcgc caattgctac aattcctgaa
     5881 gcggtagtat ttgcatttaa tgtcatcttg cctatagcaa tgttactggt gcctgtggtt
     5941 gtgttttgca gtgatttgta tcctagagac gtgtttccat tcttattgat tcctcctgcc
     6001 acagtattgt ctattttaaa ttgcaaggca acatcatctg tggttccgat aaaattggtt
     6061 ccggcaacag ttcccgcatt tccggttaaa ctccatccgg cagatcctga aatctgtgtc
     6121 catgccgctc cattgtaata ccaaaaaccg gttgagccat cggtctgata gatcaatagg
     6181 ccggtagccg gggtaggaat agccgttttt tgagcagcgg tcattcttgg aaccaaaaat
     6241 cctttagtac tggatttgat ttccagcatg gctgaattgt cggctgtaga gccatcggta
     6301 ttgatagcta tgttctgggc ctgggttccc aatacccaaa aggtcaataa gagtaagatg
     6361 atacgtttca tacaacagca tttagaatac cgcaaagtga cttattctaa agccttgaaa
     6421 ccagtagaaa tttttgaaat tgtagcagac tattccaccc aaatgaaccc tataggcata
     6481 aaaaaaggcc ccaaatggag ccttctataa agagtttgct aagtctgttt atttaagaac
     6541 tgataccttt tcagcataca caactttgcc ggcttcatcg attaaacgag caaaatacac
     6601 gccctgagga aggtcactga tatcgaaact catctttccg cttccggtat tccaatcgta
     6661 tgttctgact gtccgaccaa cgatattgac aatatcaacc ttagcgatat ttgcactgaa
     6721 ggaagctatc acattgatat tgttaaccgc aggattggga taaatggcaa aactgttgtc
     6781 aagtactgat gaaactacat caactccaca gtcctcttcg ctcacaccca aacgcgcatg
     6841 gaaaagggca accgcattat tgtcagcact gtctaaaacg ctgaaaaaat gaacttcaac
     6901 ggttccgcat ccgggaatcg gattaaaggc gccatcatga taatttctgg catcaaactt
     6961 cacatatacc gttccggttg tatctattgg cgcatcgaaa taataccccg gc
//
LOCUS       DAOQOU010000169         6903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6230_length_6903_cov_5.060456, whole genome shotgun sequence.
ACCESSION   DAOQOU010000169 DAOQOU010000000
VERSION     DAOQOU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6230_length_6903_cov_5.060456"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /gene="glgB"
                     /locus_tag="PLM90_12210"
     CDS             <1..1584
                     /gene="glgB"
                     /locus_tag="PLM90_12210"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HMZ90190.1"
                     /translation="ASIVWDSTYTWEDALWLTKRKPFTFDKPMSIYEVHLGSWGRQPD
                     DANAFLTYRQLAETLVPYVKEMGFTHVELLPVTEHPFYGSWGYQTLSFFAPTSRYGTP
                     QDLMYLIDSFHKAHIGVILDWVPSHFPQDGHGLAFFDGTHLFEHEDRRKGFHPDWNSY
                     IFNYGRFEVKSFLISSALFWVDQFHFDGIRVDAVASMLYLDYSRKEGEWIANDYGGNE
                     NLEAIQFLKELNETLYSEVPSVITIAEESTSWPKVSKPTYDGGLGFGMKWMMGWMHDT
                     LQYFKEEPVYRKYHQDKLTFSMWYAFSENFILSLSHDEVVHMKGSLINKMPGDEWLKF
                     ANLRLMFAYMFTHSFPKLMFMGGEFAQKKEWNHDQSLDWHLVEEPSHRGIQLLVKDLN
                     HLYTGEKSMYENDFKQAGFEWLDINRRDQCVLVWKRAGKQPNDHLIVVGNFDQQTHID
                     ISIPVLQRNHYVEILNTDAEKYYGSGLINPGLIKPKTVVVKNDDETEDKQYELTLTIA
                     PLTLIVLKPVAKLRRSKKS"
     gene            1607..2065
                     /locus_tag="PLM90_12215"
     CDS             1607..2065
                     /locus_tag="PLM90_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90191.1"
                     /translation="MKAFLVALLGFGILQSMTGQTAVTDPKKNEALVTIGTMSALSLY
                     NTYVAIGAVADAYGRDVYTAQNVGDLMKEQLTILPTAKAQVEKLKYSGYLEDQTDIDY
                     CQRIMDCLDLLILEAGGLSEYALHGDLDAQLNYANAREDAWELITKILQL"
     gene            2127..2732
                     /locus_tag="PLM90_12220"
     CDS             2127..2732
                     /locus_tag="PLM90_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ90192.1"
                     /translation="MKRNLLLAVICASILTLPVNAQETSLMQPSGITISAGYGFPSLA
                     KKVFDFAEGDNVNSSFFGPAYVKAEIPLSDHVGFGLNFAYSHGNATYVTQDGEIDSIF
                     YNTQFDYTSYSVLARLNFHFGQEGGMFDPYAGFGMGYRNANYAYTGGDPDAEPYDING
                     LIHLGAELTIGTRIYLTDNIGIYGEVGFAKSPFQAGLVVKF"
     gene            complement(2734..5808)
                     /locus_tag="PLM90_12225"
     CDS             complement(2734..5808)
                     /locus_tag="PLM90_12225"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90193.1"
                     /translation="MKYLASILFLLLATTELESQSIISDATIGALEGRKIGPARTSGR
                     IAAIDAVDKDPNIVYVGAAGGGVWKSINQGTTFKPIFEDFSQCIGAIAIDQQHPDTVW
                     VGTGEPWVRNSVGVGTGIYKTLNGGEKFQLMGLENSERIAGILIDPKNSNTVYVAVMG
                     ALWGDSEDRGVYKTTDGGATWTRILYVNPSTGCASITMHPTEPNTLLAAMWDHRRTAY
                     DFRSGGPGSGLYQSKDGGATWTKYQDGLPNETLGRICVSYSPVAPYTAYALVESEHSA
                     LYKYSDNDHKWLKMSEQSNMGERPFYFSLLVPDPIDPNRIYKPGFNLTVSNDGGKIFQ
                     SAAVTGGGYHSDLHALYVSPKDNRLMYLGTDGGVYISRDKGNTWSHCENLPVAQFYHV
                     SFDNADPYNVYGGLQDNGSWKGPSSSPGGIQNSDWETIGYGDGFNAYADKTDNNTVYW
                     QYQGGRVYRTNLTTGESKFIKPFPDAGTADLRYNWNAPVVFGKKSNDLYIGSQYLFRS
                     KDKGDTWQRISPDLTTNDPTRQQQEKSGGVTIDNSSAENNTTIFSICESPLDDQVIWV
                     GTDDGNVQVTVDGGKNWTLLNANIPGLPAKAFISYIDADNFEKGGAYITVDAHRNGDK
                     KAYVYYTNDYGKTWQRIDKEPIMGFCHVIKQDLVNKDLLYLGTEFGLYVSIDHGANWV
                     RFKSGVPMVGVYDMAIHPRDRDLVLATHGLGVIIIDDLTILRSITPALLDQEVAFIPT
                     RPYYFPKGGVVQDFPGDDEFIGRSRNGAAVVAYYLKKRHVFGDMFIEIYDSKGNFVRK
                     QPAGSRKGVNIVQIQTNLRPPVVPSSPAILAEAAFGPELEVGDYTIKLIKGTDTFSTK
                     LTLRYNEASNHSVADRQQRRNTMMDAYNLLERLAWYDNTILEIGAQTRETADSIKNRN
                     FEAQLLKTADRMDALHESISATQEGEGGLAAQVRLREKIAEVYSAVAGYDGKPTNVQT
                     DALAKYTTEVDAIEKELNALLHGDIETYNKALIKQGLPPIVTSTREVFMSKQ"
     gene            complement(6126..6860)
                     /locus_tag="PLM90_12230"
     CDS             complement(6126..6860)
                     /locus_tag="PLM90_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90194.1"
                     /translation="MQIRKIAAALLLLATLPVAGLKAQSLNDVLKAHFDAVGQNKVAK
                     LENMTTIGKLNQSGLDIPFKQIVARPGKLRVEGTFQGLTFIQVFNGTEGWAVNPFVGS
                     TDPQPIPADQIDDLKIQADMDGMLWDYAKKGSTVTLEGTEDVEGTPCFKIRIVTADSS
                     VFDEYIDTDSYMLIKSHSKTQMMGVDVETDTYFSNYMQKDGIAMPGRIENRYNGVTGE
                     VITIESVEFDLEYQDALFDKPAAKQE"
BASE COUNT         1711 a         1630 c         1668 g         1894 t
ORIGIN      
        1 gcttccattg tttgggacag cacttatacc tgggaagacg cactctggct aaccaaacga
       61 aagcctttca ccttcgacaa gccaatgagc atttatgagg tgcatctggg ttcgtggggg
      121 cgacagccgg atgatgccaa tgcctttctt acgtatcgtc aactcgccga aacgcttgtg
      181 ccatatgtca aggaaatggg attcacgcat gtagaactgc ttccggtaac agaacacccc
      241 ttttacggct catggggata tcaaacgctt agctttttcg ccccaacatc gcgctatggt
      301 acaccgcagg acttaatgta tctgatcgat agcttccata aggctcatat cggcgttatc
      361 ctcgattggg tgccctcaca ttttccgcag gatggccatg gtttagcatt tttcgatgga
      421 acccatcttt ttgaacatga agatcgcagg aagggatttc accccgactg gaactcatac
      481 attttcaact atggacgttt cgaggtaaaa agcttcctca taagcagcgc attattctgg
      541 gtagatcaat ttcactttga tggtatcagg gtggacgccg tggcaagtat gctgtacctc
      601 gattattcgc gcaaagaagg ggagtggatt gccaatgatt atgggggaaa tgagaatctg
      661 gaagccattc aatttcttaa agaactcaat gaaacactct atagcgaggt gccgtcagta
      721 atcaccatcg cagaagaatc cacctcctgg cccaaggtgt caaaaccaac gtacgacggc
      781 ggtctgggct ttggtatgaa gtggatgatg ggctggatgc acgatacgct gcaatatttt
      841 aaggaagagc ctgtatatcg gaagtatcat caggataagc ttactttcag tatgtggtat
      901 gccttcagcg agaacttcat cctcagcctc tcgcacgatg aagtagtgca tatgaagggc
      961 tcgctcatca ataaaatgcc gggagatgaa tggttaaagt ttgccaatct tcgattgatg
     1021 tttgcctata tgttcacaca ttccttcccg aaactcatgt tcatgggtgg tgaatttgct
     1081 caaaagaaag agtggaatca cgatcaaagc ctcgactggc atttggtgga ggagccttct
     1141 cacaggggga ttcagctgct tgtaaaggat ttgaatcacc tttataccgg agaaaaaagc
     1201 atgtatgaaa acgacttcaa acaggccggt ttcgaatggc tggatatcaa tcgccgcgac
     1261 cagtgtgtcc tggtttggaa aagggcagga aaacaaccta atgaccacct gatcgttgtc
     1321 ggtaattttg atcaacaaac gcatatcgac atcagcatac cggtgctgca acgcaaccat
     1381 tatgtggaaa tattgaatac cgatgctgaa aagtactatg gcagcggatt gataaacccg
     1441 ggtttgataa aaccgaaaac agtagtggtt aagaatgatg atgaaactga ggataaacaa
     1501 tatgaactta ccctcaccat tgcaccgctt accctcatcg tgctgaaacc tgttgccaag
     1561 ctgagaagaa gcaaaaaatc ttaatttcac tgtaaatcat atcaatatga aagcattcct
     1621 tgtagccctt ttaggctttg gtattttgca atcaatgacc ggtcagactg ctgtaacaga
     1681 tccgaagaaa aatgaagccc tggtgactat cgggacgatg tcggcactgt cgttatataa
     1741 tacttacgtt gccataggtg ctgtagcaga tgcctatgga agggatgttt acaccgctca
     1801 gaatgtcggg gacctgatga aagaacaact tacgattctg ccaacagcaa aagcccaggt
     1861 cgaaaaactg aaatactccg gttacctgga ggatcaaacg gatatcgatt attgccaaag
     1921 gatcatggac tgccttgatt tgttgatttt agaagccggt ggattgagcg aatatgcttt
     1981 gcatggcgat ttagatgctc agctaaatta cgcgaatgcg cgggaagatg cctgggagct
     2041 aatcaccaaa atccttcagc tgtaagggtt tgaaaaaaat ggcacagcgt ttggatgggt
     2101 aagtgtggct gtaatccgcc atacttatga aaaggaattt gttgttagcc gttatctgcg
     2161 caagtatact gactctacct gttaacgcac aggagacttc tctaatgcaa ccatccggta
     2221 ttactatttc cgccggatac ggttttccaa gcctggcgaa aaaagtattc gactttgccg
     2281 agggcgataa cgtgaatagt tcctttttcg gacctgcata tgtgaaagct gagatccccc
     2341 tgagtgatca tgtagggttt ggactcaact tcgcttacag ccatgggaat gccacctatg
     2401 taactcagga tggcgagatt gacagtatct tttacaatac ccaattcgac tatacctctt
     2461 atagtgtgct ggcgcgattg aacttccatt tcggacagga gggaggcatg tttgatccgt
     2521 atgcaggttt tggaatgggt taccgcaatg ccaattacgc ctatacaggt ggcgaccccg
     2581 atgctgaacc ttatgatatc aatggtttaa tacatctggg tgccgagctt accataggta
     2641 cacgcattta ccttaccgat aatattggta tttatgggga agtaggcttc gccaaatcgc
     2701 ccttccaggc agggttggta gtgaaattct aacctattgc ttgctcatga atacctctcg
     2761 ggtagaggta acgatagggg gtaagccctg tttgatcaat gccttgttat acgtttcgat
     2821 atcgccgtgt agcagggcat tgagttcttt ttcaatggca tcgacctcag tggtatattt
     2881 tgccaatgca tctgtttgca cattcgtcgg cttgccgtca taaccggcaa ctgccgaata
     2941 cacttcagcg attttctcac gcaaacgcac ctgcgccgcc aatccgcctt cgccttcctg
     3001 cgtggcgctg atgctttcat gcagggcatc catgcgatca gctgttttca ggagttgagc
     3061 ttcgaagttc ctgtttttga tggaatcagc agtttcccgc gtttgggcgc cgatttccag
     3121 aatcgtattg tcataccagg caaggcgttc gagcaggttg taggcatcca tcatggtatt
     3181 cctgcgttgc tgacgatcgg ctaccgaatg gtttgaagcc tcattatatc gcaaggtaag
     3241 cttggtgcta aaggtgtctg tgcctttaat caatttgatg gtatagtcgc cgacctccaa
     3301 ctccggaccg aaggcagcct ctgcaagtat ggcaggggag gatggcacaa ccggtggcct
     3361 caggtttgtc tgtatctgca cgatattcac acccttgcga ctcccggcag gctgtttgcg
     3421 cacgaagttg cctttgctgt cgtagatctc gatgaacata tcgccgaaaa catggcgttt
     3481 tttgaggtaa taggccacaa ccgcagcgcc attcctgctg cgcccgatga attcatcatc
     3541 tcccggaaag tcctgaacta cccctccttt tgggaagtag tatggtcggg tggggatgaa
     3601 agccacttcc tgatccagta aggccggggt gatgctgcgt aatatcgtaa ggtcgtcaat
     3661 gatgatcacg ccaagtccat gagtggctaa taccaggtcg cgatcgcgag gatgaatggc
     3721 catgtcatac acacccacca tcggcacacc cgatttgaaa cgcacccagt tagccccatg
     3781 atcaattgaa acatataaac cgaattctgt tccaaggtat agcagatcct tgttgacaag
     3841 gtcctgcttg atcacgtgac aaaatcccat aattggttct ttatcaatac gttgccatgt
     3901 tttaccgtaa tcattagtgt agtaaacata tgccttcttg tcgccattgc gatgtgcgtc
     3961 tacagtgatg tatgcgccgc ctttttcgaa gttatcggcg tcgatatacg agatgaaagc
     4021 cttagcagga aggccgggaa tatttgcatt cagcagggtc cagttctttc cgccatcaac
     4081 agttacttgc acattgccgt cgtctgttcc tacccatatc acctggtcat cgagtggcga
     4141 ttcgcagata ctaaaaatgg tggtattgtt ctcggcggag gagttgtcga tggtaacacc
     4201 accgcttttt tcctgttgct gacgcgtagg atcattggta gttaggtccg gactgatacg
     4261 ctgccaggta tctcccttgt ctttggaacg aaataaatac tggctgccga tatagaggtc
     4321 gttcgatttc ttgccaaaca caaccggtgc attccagtta taccgcaggt cggccgtgcc
     4381 ggcatcagga aagggcttga tgaatttact ttcgccggtg gtcaggttgg tgcggtaaac
     4441 tcgaccacct tggtattgcc agtaaacggt attgttgtct gtcttgtcgg cataggcatt
     4501 gaacccatcg ccataaccta ttgtctccca atctgagttc tgtatacctc cgggggagga
     4561 tgaaggacct ttccaactgc cattgtcttg cagaccgcca tacacattgt aaggatccgc
     4621 attgtcgaag ctcacgtgat aaaattgcgc aaccggcagg ttctcgcaat gactccaggt
     4681 attccctttg tcgcgggaga tgtaaacacc gccatcggtt cccaaataca ttaagcggtt
     4741 atctttcgga ctcacataca aggcatgcaa atcgctgtgg tatccaccac cggttaccgc
     4801 tgcgctttgg aagatcttgc cgccatcgtt gcttacggtc aggttgaagc cgggcttgta
     4861 gatgcggtta gggtcaattg gatcgggtac gagcagacta aagtaaaacg gacgttcacc
     4921 catgttgctt tgttcgctca ttttcagcca cttgtgatcg ttatctgaat acttgtataa
     4981 tgctgaatgt tcagactcca ccaaggcata ggcggtatat ggtgccaccg gtgagtagct
     5041 tacacagatc ctgccaaggg tttcattggg taatccgtcc tgatattttg tccacgttgc
     5101 cccaccatcc ttgctttggt aaagtccgct ccccgggcct cccgacctga agtcgtaagc
     5161 ggttcggcga tggtcccaca tagctgcaag cagggtattg ggttcggtag gatgcatggt
     5221 aatagaggcg caaccggtag atgggtttac atacaatatc ctggtccagg ttgctccgcc
     5281 atcggtggtc ttatacaccc cgcgatcctc actatcaccc cataaggcac ccattaccgc
     5341 cacgtaaacg gtattcgaat ttttcgggtc gatgaggatg ccggcgatgc gttcgctgtt
     5401 ttcaagtccc atgagctgga acttctcccc gccattcaag gtcttataaa ttcccgttcc
     5461 cacacccaca gagtttctca cccatggctc gccggtcccc acccaaacgg tatcgggatg
     5521 ctgctggtct atggcgatgg cgccaatgca ctgactaaaa tcttcaaaaa tgggcttgaa
     5581 tgtcgtcccc tggttgatcg acttccacac accgccgccg gcagcgccca catatacaat
     5641 gttcggatcc ttatccaccg catcaatcgc cgctatgcga ccacttgtcc tcgcaggacc
     5701 aatctttctt ccctcaagag ccccaatagt agcatcagaa atgatcgact ggctctctaa
     5761 ctccgtggtt gcgaggagga ggaatagtat gcttgctaag tatttcattg ttagtgggtg
     5821 agcttgcctc gccgataggc gggtgggtga gtgtttcgtg aatcgtgact cgtgaatcgt
     5881 gagtgtggtt cggagggtaa tattttgatt aacttgcttt gcctattgtt ggtgagtggt
     5941 tactgatcca ctgactccgc taactccgca aactccgcga ttgaactagg gtgagtggtg
     6001 agtttgcatt cctctgcgcc tccgcgtctc tgcgtgaaac aatactacgt tacatgatat
     6061 aaacgactat aaatcattct attacagtca tttataaaaa atgcatatac ataatttata
     6121 aattattatt cttgtttagc cgccggttta tcgaacaaag cgtcttggta ttctaagtcg
     6181 aactcaacag actcgatggt gatcacttcg ccggtcacac cgttgtagcg gttttcaatt
     6241 cttcccggca tggcgatgcc atctttttgc atatagttac tgaaataggt atctgtttct
     6301 acgtcaaccc ccatcatttg ggttttgcta tgcgatttga tgagcatgta agaatcggta
     6361 tcgatgtatt catcaaaaac cgaagaatcg gcagtcacaa tcctgatttt gaaacaagga
     6421 gtgccttcca catcttcggt gccctccaaa gtaacggtgc tgcctttttt ggcataatcc
     6481 cacaacatac cgtccatatc tgcctggatc ttcaggtcgt cgatttgatc ggcagggatg
     6541 ggttgtgggt ctgtagaacc gacaaatggg ttcacagccc atccttcggt gccattgaaa
     6601 acctggatga aggtgaggcc ctgaaaagta ccttcaaccc ttaacttgcc cgggcgcgca
     6661 acaatctgct taaatggaat atccaaaccg ctctgattga gcttacctat ggtggtcata
     6721 ttttccagtt ttgccacttt gttttgtcct acggcgtcaa aatgcgcttt tagcacatcg
     6781 ttgagtgatt gggcttttag tccggccacg gggagggttg ccagcaacaa caaagctgcg
     6841 gcgatctttc tgatttgcat gatgagttga aattgtgggt taaaattacg acctgtctat
     6901 gtg
//
LOCUS       DAOQOU010000170         6881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6263_length_6881_cov_5.713742, whole genome shotgun sequence.
ACCESSION   DAOQOU010000170 DAOQOU010000000
VERSION     DAOQOU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6263_length_6881_cov_5.713742"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLM90_12235"
     CDS             complement(<1..627)
                     /locus_tag="PLM90_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90195.1"
                     /translation="MKKYTLLVLTLAISMGLKAQYPLVEIYDIQYKDAAALAAEDDLS
                     AYDGDTVRIQGIVTFNPCDYALSSTGSRMGTWLQDEDGGPFSGIHVLIDFPAIGYTDL
                     ESLNDATLFVDNFQVGNIVECTGIVNSFEGNTQFLLLPIETSIVGFTTLPTPVPAEVS
                     ELEVSDGAGGQVINTVDGEQYEGRYVEFTNVYVTDVTPSGLRWFWYLQD"
     gene            complement(695..1336)
                     /locus_tag="PLM90_12240"
     CDS             complement(695..1336)
                     /locus_tag="PLM90_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90196.1"
                     /translation="MYTLPKFTKSLAMVAAAILLTVSTSLYAGNGNYRNSPYGRDPNP
                     VRVYYFGYDLTNPGFGIGTEVNLSWTKMEKSGCKGSRISDRQFMMIPNLGMFTNEAGS
                     QSIFGNLEFAYAVTYRHGLTLEVFGAGGYAQMLSELTNDQADNSKITNGTASETAYSG
                     FMPSAGVGIGYDFQKLNGKDFPLELNFRGLATSTNIGESIITPAFQTGLIYSF"
     gene            complement(1583..3250)
                     /locus_tag="PLM90_12245"
     CDS             complement(1583..3250)
                     /locus_tag="PLM90_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HMZ90197.1"
                     /translation="MKSLCTALLICLSLPTWAQKSIVVNTDIEKATVFLNGAQLFHTA
                     SVHIPSGPSEIVLQGFSNSLDQNSIQGGGKGDFTILDIQYRLFYPEPVDVTPELSAID
                     KKIKIKQDSIVEVDYDLRAIAAKQEALNVQKMMLMNNALLHNTNNDSLELLQHSIAYY
                     ESKLSEVLDKLLELDKQSYVLNNRRAAITARISELYAYRSQQAAGSNPNAMIPQVVMQ
                     VYADASVDAKMEVNYITYNAGWYATYDIRANDIAKPVELAYKANVWQNSGIEWKNVKL
                     TCSTGNPMLGNTLPELTAWYLGYYQYYYDRDVQTLSGATAPAAATKSLEEVAGYDDFK
                     DESLQKSTNASNFTTQNATVANVEFEINLKYTVPSDGKGHVIALQSEQLKTKYNYLII
                     PKLEQSAFLIARITDWEKINLLPGNANIYFNNTYVGKTYLNPLTLSDTLSVSMGRDRS
                     IEVKRELLADKSTDKILNAKAHKTMSYTIDVRNTKAIGIEIILKDQVPLSNDDDVKVE
                     VLEQSAAQLNEDTGILTWRETLASKQKKTWTFGFEVSYPKDEPLSLQ"
     gene            3433..3888
                     /locus_tag="PLM90_12250"
     CDS             3433..3888
                     /locus_tag="PLM90_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90198.1"
                     /translation="MACNHTNSSTSPEDFANTLSIQHIDAENAIMRQNIERICKDSNC
                     AYFSFYGKSCKNLIDTISTLYSKYQNIDGKVHFSVFMNEKRSCLQIFGNQSETHGLYS
                     EHITHAYLADTIISGDAAILQLELLEQDILERYNPIKRVISSNCGLMIY"
     gene            complement(3882..5198)
                     /locus_tag="PLM90_12255"
     CDS             complement(3882..5198)
                     /locus_tag="PLM90_12255"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HMZ90199.1"
                     /translation="MPEINNIPVAIHWFRRDLRLTDNVALSLALRKHERVIPLFIFDK
                     EILDQLEPDDRRVTFIHDVLLQMDATLKTWGSSLLVKYGAPADVWRSLIAEYHIEAVY
                     TNHDHDPYALLRDGNIGALLADHHIPFTTCKDQVIFEKDDVLKDNGTPYTVYTPYSVK
                     WKSLFKEEYLNEHQIFPFRKHFHQFQATPIPSLAMIGFDGERAEIPKMLPDEVIIRNY
                     HNTRDLPAINGTSRLSVHLRFGTISIRSLMRETRHLSEKYFNELIWREFYQMILAQFP
                     HVITNSFRPEYDRIQWRNNEREFDAWCQGKTGFPIVDAGMRELLATGHMHNRVRMIVA
                     SFLTKDLLIDWRWGEAWFAKHLLDFDLASNNGGWQWAAGCGVDAAPYFRIFNPYEQAK
                     KFDPEGKYIRKWIAEIDELTYPQPIVDHAFARKRTLDAYKIALSNQ"
     gene            complement(5191..6024)
                     /locus_tag="PLM90_12260"
     CDS             complement(5191..6024)
                     /locus_tag="PLM90_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HMZ90200.1"
                     /translation="MVFYNHNYIQDDALHLLPSNRAFKYGDGIFESCLLNEGALPLMA
                     YHTTRLMQGMQCLHMDALPHLSEKWLGDVIKELALQHGYQSARAKISVWRGGEGLYLP
                     KSDSPELLIEVFPLHSTPFSEAPQQYVIDIYDGLQKAIHPVSACKTMNALPYILAAKF
                     AASHKWNDVLILNTQNEIADAISSNVWLIKDHMLITTPALNGGVEGTMQAWLLDHVEE
                     LGFSASRKSLTISDVESADAVFLTNAIQGIIPVTQFREKTFPNNLPLALLRDVKTLLF
                     A"
     gene            complement(6042..6695)
                     /locus_tag="PLM90_12265"
     CDS             complement(6042..6695)
                     /locus_tag="PLM90_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HMZ90201.1"
                     /translation="MLDKNQIAKRIAQELKDGYYVNLGIGIPTLVANYIPSGMTVTLQ
                     SENGLLGMGPFPKDEDVDADIINAGKQTITTLPGSAFFDSAMSFGMIRAGKVDLTVLG
                     AMEVADNGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKEGQSKLLKSCTLP
                     ITGRGVVKKIVTELGVFDVTPQGFKLLERAPGVSVEEIQSKTEGRLVVEGDIPEMIL"
     gene            complement(6719..6874)
                     /locus_tag="PLM90_12270"
     CDS             complement(6719..6874)
                     /locus_tag="PLM90_12270"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HMZ90202.1"
                     /translation="MAGKITIAEVEELVPAGQLDPNNIHTPGIYVHRIFQGADYEKRI
                     EQRTISK"
BASE COUNT         1981 a         1475 c         1381 g         2044 t
ORIGIN      
        1 atcctgtaag taccagaacc aacgcaatcc cgaaggtgta acatcagtaa catacacatt
       61 cgtaaactcc acataccgtc cttcatattg ctctccgtcg actgtattaa ttacctgacc
      121 gcctgcgcca tcactcactt ccagctcgct cacttcagca ggaacaggag tcggcaaggt
      181 tgtgaagcca acaatacttg tttctatcgg taacagcaag aattgagtat ttccttcaaa
      241 ggagttaaca atacctgtgc attctacgat attccctacc tggaaattat ctacaaacaa
      301 ggttgcatca tttaaagatt caagatcggt ataaccgatg gcagggaagt cgatgagtac
      361 gtgaattcct gagaatggac cgccgtcttc atcctgcaac catgttccca tgcggcttcc
      421 ggtagatgac agggcataat cgcaagggtt aaaagtaacg ataccttgta ttctgaccgt
      481 atcgccatcg tacgcagaaa gatcatcttc tgcagccagg gcggcagcat ctttatattg
      541 gatgtcgtag atctccacga gagggtattg agccttcaga cccatagaaa tggcgagggt
      601 caataccaga agtgtatatt ttttcataaa aaaatttggg gtcaaaggta atgaaaccct
      661 tgaccccaaa gaagaatttt atctgaatta ttcgttaaaa gctatagatc aaacctgtct
      721 gaaaagcagg agtgataata ctttcaccaa tatttgtaga agtggccaac ccacggaaat
      781 tcagttccaa cggaaaatct ttgccattga gtttctggaa atcgtagcca atgccaactc
      841 cggcagatgg cataaaaccg ctgtaggccg tttctgatgc tgttccgttt gtaattttag
      901 agttatcagc ctggtcgttg gtcaattcgc tgagcatttg ggcataacca ccggcaccga
      961 atacctcaag ggtaagtccg tggcgatatg ttaccgcata cgcaaactca aggtttccga
     1021 agatactttg agaaccggcc tcattggtga acataccaag attaggaatc atcataaact
     1081 ggcgatcaga tatacgagaa cctttacagc cgcttttttc catttttgtc cagctgaggt
     1141 taacttccgt gccaattcca aaaccgggat tggtaaggtc ataaccaaaa tagtaaacac
     1201 gaacagggtt aggatcgcgt ccgtaaggtg aattcctgta atttccgttg ccggcataca
     1261 ggctggtaga taccgtcagg aggatagctg cggccaccat tgcaagtgat tttgtgaatt
     1321 tgggcagggt gtacatcgtt cgttgttgaa aaggaaggtg caaaattaac gctttctaaa
     1381 tcataacatt gtaaaaagac ttttaattcc gacaaagacc aatatttttt atggttttat
     1441 tgagctccat cggaaacata tgtaaaatta cacgatttaa gcgcagccta aacctctgtt
     1501 atccacaccc attcagggat atcaaacaca aagcagcacc ccgacttgag cgccggggtg
     1561 ctgctgcact tacccaatca gcttattgca aactgagtgg ttcgtctttt ggataactta
     1621 cttcgaatcc gaaggtccag gttttcttct gtttggaagc gagggtttct ctccaggtga
     1681 ggataccggt atcctcgttg agttgagcag cactctgctc aagcacctct accttcacat
     1741 cgtcatcgtt tgaaagaggc acttggtctt ttaggattat ctcaatgcca atggctttgg
     1801 tattgcgcac atcaatggta taactcattg tcttatgtgc ttttgcattc aaaattttat
     1861 cggttgattt atcggcaagc aattcacgct tcacttcaat ggatctgtcg cgacccatgg
     1921 atactgaaag tgtatcactt aaagtaagag gattaagata tgttttgcca acgtaggtat
     1981 tgttgaaata aatattggca ttgccgggaa gtaaattgat tttttcccaa tctgtaatgc
     2041 gtgcaatcag gaatgcagat tgttcaagct tcggaatgat cagataatta tattttgttt
     2101 tcaattgctc tgattgcaag gcaataacat gtccctttcc atcacttggc acagtatact
     2161 tcaagttgat ttcaaattcg acattggcaa cagttgcatt ttgtgtggtg aagtttgatg
     2221 catttgtaga tttttgcaac gattcatctt taaaatcatc atatcctgcc acttcctcca
     2281 atgattttgt tgctgcagcc ggagctgttg cgcctgataa tgtttgcaca tcgcggtcgt
     2341 aataatattg atagtagcca agataccatg ctgtgagctc aggcaatgta ttacccagca
     2401 taggatttcc ggttgaacat gtcaatttca catttttcca ttcgataccg gaattctgcc
     2461 aaacatttgc tttgtaggca agttcaaccg gtttggcgat atcgttagca cgaatatcat
     2521 atgttgcata ccaacctgca ttgtacgtga tgtaattcac ttccattttt gcatcaacgg
     2581 aggcatcagc ataaacttgc ataaccactt gaggaatcat agcgttggga tttgatccgg
     2641 ctgcttgctg actcctataa gcatatagtt cagatatcct tgcagtaatt gctgctcttc
     2701 tgttgttcaa tacatatgat tgcttgtcga gctctaataa tttgtccaac acctcactca
     2761 attttgattc ataatatgct attgaatgtt gaagtaattc aagtgaatca ttatttgtat
     2821 tatgtaaaag tgcattgttc atcaacatca ttttctgcac attcaaagcc tcttgctttg
     2881 cagcaatagc gcgcaaatca tagtctactt caacaattga atcttgtttg atttttattt
     2941 tcttgtcaat tgccgataat tcgggtgtga catcaaccgg ttcggggtag aacaatcgat
     3001 attggatgtc cagaatagta aaatcacctt tccccccacc ctgtatggag ttttggtcga
     3061 gggaatttga aaagccctgc aggacgattt ccgaaggtcc tgaggggata tgaactgaag
     3121 ccgtatgaaa tagttgagcc ccattcaaaa acacggtggc cttttcaata tcagtattca
     3181 caacgatgga cttttgggcc catgtaggca aggaaaggca gatcagaagt gcagtgcaga
     3241 gagatttcat aatattgttt tgcagtatga tgcaatggta tcatggattc cataaaccaa
     3301 taaaaatttt aatgatctgg tagccatact tgctgatata cctgaatgcc attcgatttc
     3361 gttatgaatg gaccggtatc caaatgcaca tttatgcgta tagtaatcct gtatatcctg
     3421 ctcattacct gcgtggcctg taatcataca aattcatcaa cttcacctga agattttgct
     3481 aatactttga gcatacaaca tattgatgct gaaaatgcaa taatgcgaca aaacatagag
     3541 aggatttgca aagattcgaa ttgtgcatat tttagctttt atggcaagtc ttgcaaaaac
     3601 ctcattgata caatttcaac tttgtactca aaatatcaaa atattgatgg taaagtacat
     3661 ttttctgtat ttatgaatga aaaacgttca tgtttacaaa ttttcggcaa ccaaagtgaa
     3721 acgcatggcc tatacagcga acacattaca catgcttacc tggcagatac aattatttca
     3781 ggagatgctg ctattctaca attagaattg cttgagcaag atattctgga aagatacaac
     3841 ccaataaaaa gggtaatatc ttcaaattgt ggcttaatga tttattgatt cgacaaagca
     3901 attttatatg catcaagcgt acgctttctc gcaaaggcat ggtctactat cggttgcgga
     3961 taggtaagct cgtcaatttc tgcaatccat tttcgaatgt atttaccttc cgggtcaaac
     4021 ttttttgctt gttcgtatgg attgaaaata cggaaatatg gagcagcatc aacaccacat
     4081 cccgctgccc actgccatcc accattatta cttgcgaggt cgaaatcgag gagatgcttt
     4141 gcaaaccacg cttcacccca tcgccagtca attaaaaggt ctttggtaag aaaactggca
     4201 acgatcatgc gtactcgatt atgcatgtga cctgttgcga gtagctctct catacctgca
     4261 tctacaattg gaaacccggt cttaccttga caccatgcat cgaattcacg ctcgttgttt
     4321 cgccattgaa tgcgatcata ttccgggcgg aaagaatttg tgataacatg cggaaattga
     4381 gcgagtatca tttgataaaa ttctcgccaa atcaattcat tgaaatattt ttcagataaa
     4441 tgtcgtgttt cacgcatcaa agaacgtatg ctgattgtgc caaaccgcaa atgcacactc
     4501 aatcgcgaag tgccatttat tgcaggcaga tcgcgggtat tgtgataatt gcgaatgatg
     4561 acttcgtcag gcaacatttt aggaatttca gctcgctcgc catcaaatcc tatcatggca
     4621 agcgaaggaa tgggagttgc ctgaaattga tgaaaatgtt tgcgaaatgg aaaaatctga
     4681 tgttcattga gatattcctc tttaaacaac gatttccatt ttacagaata aggtgtgtat
     4741 accgtatatg gcgtgccatt gtccttcaac acatcatctt tttcaaatat cacctgatcc
     4801 ttgcaagtgg taaacggaat atgatgatca gctaacaatg caccaatatt gccatcgcgc
     4861 agcaacgcat acggatcatg atcatgattg gtatatactg cttcgatgtg atattctgca
     4921 atcagcgagc gccacacatc agcaggtgca ccgtatttca ctaataagga cgaaccccac
     4981 gttttaagtg tggcatccat ctgcaataaa acgtcatgaa taaaagtcac cctgcggtcg
     5041 tcaggctcca gttgatccag aatttcctta tcgaagataa ataatggtat aactctttca
     5101 tgcttgcgaa gtgcgagaga aagggcaaca ttatctgtca atcgcaggtc gcgacgaaac
     5161 caatgaattg ctacaggtat attgtttatt tcaggcaaag agcaaggttt tcacatctcg
     5221 taacaaggct agaggcaaat tgttcggaaa tgttttctcc ctgaactgcg taaccggaat
     5281 gatcccttgt atggcattgg tgaggaaaac agcatcagca gattcaacat cagaaatagt
     5341 taaagatttg cgtgaggccg aaaatcccaa ttcctctaca tggtcaagca accatgcttg
     5401 cattgtacct tccacaccac catttaaagc aggagttgtg atgagcatat ggtcctttat
     5461 caaccaaaca tttgagctga tggcatcggc aatttcgttt tgagtgttta agatcagcac
     5521 gtcattccat ttatgggatg ctgcaaattt cgctgcaaga atgtagggaa gtgcgttcat
     5581 tgttttgcaa gcgctgacag gatggatagc tttttgcaaa ccatcatata tatctatcac
     5641 atattgttgc ggagcttctg agaatggcgt tgaatgcaag gggaaaacct caatcagcaa
     5701 ctccggtgaa tcacttttgg gcaaatacag accttcgcct ccacgccaaa cagaaatttt
     5761 tgccctagca ctttgatagc catgttgaag tgccagttct ttaatgacat cacccaacca
     5821 tttctcagaa agatgaggca gggcgtccat gtgaagacat tgcatacctt gcattaaacg
     5881 ggtggtatgg tatgccatta atggcaaagc accttcattt agcagacaac tttcaaaaat
     5941 gccatcgcca tatttaaacg cgcgatttga tggcaacaga tgtaaggcat catcctgaat
     6001 gtaattgtga ttataaaaca ccataattca ggattgcatt ttcagagtat catttcagga
     6061 atatcacctt ccactaccag gcgaccttct gtttttgatt gaatttcttc gactgacaca
     6121 cccggagcgc gttcaagtag tttgaagcct tgaggtgtta cgtcgaatac acccaattcg
     6181 gtgacaattt tcttcaccac tcccctacct gtaattggca aggtgcatga tttaagcagt
     6241 ttactttgcc cttccttatt cacatgttgc atagcaacaa taatattttt tgcactggct
     6301 accagatcca tagcgccgcc cattcccttc accatttttc ccggaatttt ccaattggca
     6361 atgtcgccat tatcagccac ttccatagca cctagaacag taagatcaac tttcccggcg
     6421 cggatcatac cgaagctcat tgcgctatcg aaaaatgcag aacccggcaa ggtggtgata
     6481 gtttgcttac cggcatttat aatgtcggca tcaacatctt catcctttgg gaatggtccc
     6541 atacccaaca agccgttttc actctggagg gtaacagtca ttccggaagg aatataatta
     6601 gcaacaagag ttggaatacc tattccgagg ttaacataat aaccatcttt caactcctgc
     6661 gcaatgcgtt ttgcaatttg attcttatct agcataattc cttttactcc ttataaaatt
     6721 atttactgat ggtgcgttgt tcgatgcgtt tttcataatc tgcaccttga aatatccgat
     6781 gtacataaat tcccggcgtg tgaatattat ttgggtcgag ttgacctgca ggcaccagtt
     6841 cttcaacctc cgcaatagta atttttcctg ccatggccat t
//
LOCUS       DAOQOU010000171         6837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6335_length_6837_cov_5.037747, whole genome shotgun sequence.
ACCESSION   DAOQOU010000171 DAOQOU010000000
VERSION     DAOQOU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6335_length_6837_cov_5.037747"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1405)
                     /locus_tag="PLM90_12275"
     CDS             complement(59..1405)
                     /locus_tag="PLM90_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMZ90203.1"
                     /translation="MSTIQLKTSIPGPKSQEIIERRKNAMPAGLAKSTEVAVVSAEGG
                     VVTDADGNILLDFAGGIGMMNIGHRHPDVVKAMQEQLDKYIHVCALVATPEPYVELAE
                     MLNALTPGNFPKKTILANSGSEAVENAINIARYATKRSAVICFEGGYHGRTLLTLSLT
                     SKYALFKKGFGPFVSDVVRLPAPNMYRKPQQFSEAEYIQFCIQQFDNAMIAQIDGDSV
                     AAIIIEPFQGEAGFIPMPKEFLQHLRNVADKHGIVLIFDEIQCGASRTGKFFACEHAG
                     VIPDMICMAKSIGAGMPISAVTGRASIMDAPHLGGVGGTYGGNPIACVAAIEAVKVLS
                     STEFLNRVQEVGNLISAQLQNWKEKYALIGDVRGIGAMQLVEFVSNRNERTPDPDVTL
                     EIIRDAVAHGLLMIRAGLYSNCIRLLPPVVMTDAQLQEGLAVLEAAIARAHNKRGL"
     gene            complement(1416..2051)
                     /locus_tag="PLM90_12280"
     CDS             complement(1416..2051)
                     /locus_tag="PLM90_12280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ90204.1"
                     /translation="MEIINEIISGNIIIIHTRAEHAQALEDLQYIVFPNLAEDEILHK
                     AHYLKHLEIFPEGQLVALHNDRVIGGTTTMRYHMDPDHPAQHTFSETVAGGWLTTHQP
                     AGEWLYGIDVSVHPDYRNRGIARAFYNIRQQIAKALGCRGQLTVGMLNGYFNYASTMS
                     VKTYFEKVKAHELFDPTVSVQEKIGFRIVSLMENYLNDPTCGNAGAVIVME"
     gene            complement(2062..3462)
                     /locus_tag="PLM90_12285"
     CDS             complement(2062..3462)
                     /locus_tag="PLM90_12285"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HMZ90205.1"
                     /translation="MEHLATDYGWLALIPPLVVIIVSIMLKSSFEGLLIGCVTGFIIV
                     DGWGFFDGFITSLYEVMQSTDTIWVIVVCGLYGSVMAMMIRSGGAMQFGERMLRYMKT
                     KRQALMGTWLLGIFVFIDDYLSALTTGITMRAVTDKFKIPREYLAYIVNTLSPPLCVI
                     VPISTWTLFIGVQLENSGFAAEGQGLQKYMSVIPFVAYGWISIFMVPLFIYGILPHFK
                     KMKQAIARAEETGLTVPVGIKLSTVQFETFPGLVHPKARYFLLPMIVVLGATVAFQDP
                     SVPGIEIDALKGVIIGVAFTFFYFLYMKLSNVQHLTETIFSGFNSMLFALALVVVSLM
                     LKDVGDKMGLTQYVINSVTPYVSSAFLPVSIFIALSLISFTTGSSWGLYVVAIPIVIP
                     LAQSIDSNVLLNIGAIISAGVFGANACLYSDATVLTSQSCETNNMQHGVSQLPYALTS
                     WGVSVLAYIILGYVMA"
     gene            complement(3519..4943)
                     /locus_tag="PLM90_12290"
     CDS             complement(3519..4943)
                     /locus_tag="PLM90_12290"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HMZ90206.1"
                     /translation="MQFKQYINGNWCDAANAGTQQVIDPANGEYVQTVPYGDGNDTTI
                     AIDAAHDAFRTWKKMSPWTRADILQKTANYIRQHIVEFATLMVRESGKPLAEAKGEWT
                     VAANLFEWYAEEGKRAYGTVIPSARTDKRMQVILQPMGVIGVITAWNFPAYNPARAWA
                     AALAAGCTIVAKVSEFTPLSGMLLINALEASGIPNGVVNLVNGDAANTGKILLQDARV
                     RKISFTGSTRVGKILMSGAAETNTKLALELGGNAPVIIMPDVDIDHVAKTAVAGRFRN
                     CGQVCIAPQRFFVHADIIDAFQERVVYYTAQLNVGHGLEPETRIGPLINSIQQDHVLS
                     IITEAKAEGNEVLTGGHSIAGGHFVAPTVIRVKNVASVFYDREIFGPVMPLIPFNDID
                     EVIKSANETPYGLAAYVWTNNINHATYISEALEFGIVGINEWAAHATEAPFGGWKQSG
                     LGHESGREGLLEYMEKKLISIGGL"
     gene            complement(4952..6271)
                     /locus_tag="PLM90_12295"
     CDS             complement(4952..6271)
                     /locus_tag="PLM90_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ90207.1"
                     /translation="MSTEVVMNKTASLYERRKQLVPNALGIFNPSSISHASGAIVYDA
                     DGRALIDFAGGIGVLNAGHCPEPVVRAIQQQAEKFIHACFNVSIYEQYLDAAQHMIDI
                     LPHGDATKIMFTNSGAEAIENAIKIARQATGRSAVIAYTQAFHGRTLMAATLTSKVGY
                     KTGFGPYAPEIYRIDFPAFAAYEGPLDEDSFSDLHIENLRSFFNTHIPAKQVAAIILE
                     PVQGEGGFHVVPKHYLQQLRNLCNEHGIVLILDEVQTGFGRTGSWGAYQQLGVIPDIS
                     TWAKSMGSGMPVGAVMGKAHIMDAAIAGTIGGTYLGNPLCMVAVSATINYMKEIDINA
                     KGMHVGSIIRKRFMQLRDNYPQHISSVRGMGAMMAFELSYDGDIRKPNGDLCKKITQR
                     CYENGVIAISSGIHGNVIRNLAPLVITDEQLESGLDILEEAVAYYCK"
     gene            complement(6268..>6837)
                     /locus_tag="PLM90_12300"
     CDS             complement(6268..>6837)
                     /locus_tag="PLM90_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ90208.1"
                     /translation="IASKRFIVEEKIRPDFIQALINGISAMKQGDPFNADTTIGPMAR
                     MDLAAQLESQLQISLRKGAGLLAGGARKGANFLPSVLDHVGKGMPAFDEELFGPIAAV
                     ISAANAEEAIAMANDSRYGLGASIFTQDMDKAKHYARQIESGSVFVNALVKSDPRIPF
                     GGIKRSGYGRELSHFGMKEFMNIKTIYIA"
BASE COUNT         1937 a         1608 c         1310 g         1982 t
ORIGIN      
        1 atgttgaata tcctgtgtat gcacatgcgc attatgtaac aggaggcgac tcatatattt
       61 acaatccgcg tttgttatgt gcacgtgcga tggctgcttc gagtacggca agtccttctt
      121 gcaattgtgc atcagtcata actacaggag gaagcaatcg aatacaattt gaatataatc
      181 cggcgcgaat cattaataat ccatgtgcaa cagcgtcgcg aataatttct aaggttacat
      241 caggatcagg ggtgcgttca tttctattgc tcacaaattc gacaagttgc atagcaccga
      301 ttccgcggac atcacctatc aatgcatact tttctttcca attttgaagc tgtgctgaaa
      361 ttaaattacc aacttcctgc acgcgattta aaaattcagt tgatgataac actttcactg
      421 cttcgatagc agcgacacat gcaatgggat ttcctccata ggtacctcct actccaccaa
      481 gatgcggtgc gtccatgatc gaggctcttc ctgtaactgc gctgatcggc ataccggcgc
      541 ctattgattt cgccatacat atcatatccg gaattacgcc tgcatgttca catgcaaaga
      601 atttaccggt tcgcgaagca ccacattgta tttcatcaaa tattaataca atgccatgtt
      661 tatcggcaac atttctgaga tgttgaagaa attctttcgg cattgggata aaccctgctt
      721 caccctgaaa cggctcaata ataattgctg caacgctgtc gccatcaatt tgagcaatca
      781 ttgcattatc aaattgttga atacaaaatt gaatatactc cgcttccgaa aattgttgtg
      841 gcttgcgata catatttggc gcaggaagac gcacgacatc acttacaaac ggaccaaagc
      901 cttttttaaa taaagcatat ttactggtga gcgataatgt caacaaagtg cgaccatgat
      961 atcccccctc aaaacaaatt acggcgcttc gttttgtggc atagcgcgca atattaatgg
     1021 cattttccac tgcttcacta ccgctgttgg cgaggattgt tttctttggg aaatttcccg
     1081 gagtcaatgc attcaacatt tcagccagtt caacatatgg ttcgggtgtg gctactaatg
     1141 cgcaaacgtg aatgtattta tccaactgtt cttgcatcgc tttcacaaca tcaggatgcc
     1201 gatgaccgat attcatcatg ccgataccac cggcaaaatc gagcagaata ttgccatcag
     1261 catctgtaac aacaccacct tcagcggaga ctacggcaac ttcggtagat ttggccaaac
     1321 ctgcaggcat ggcatttttc cgacgttcaa taatttcctg tgatttcgga ccggggatac
     1381 ttgtttttag ttgaatggtt gacatatcat ttattttatt ccattacaat aaccgcacct
     1441 gcatttccgc aagtgggatc gtttaaataa ttttccatta agctgacaat tctgaaacca
     1501 attttctcct gcactgaaac ggtaggatca aataattcat gggcttttac tttctcgaaa
     1561 tacgttttca ctgacatagt acttgcataa ttgaaatacc cgttcaacat accaacagtc
     1621 agctgaccgc gacaaccaag cgccttggca atttgctgac gaatattata aaatgcgcgg
     1681 gcaatacctc tgtttctata atcgggatgc acacttacat cgatgccata taaccattca
     1741 ccggcgggtt gatgtgtggt gagccatcca cctgctactg tttctgaaaa tgtatgttga
     1801 gccggatgat cgggatccat gtgatatcgc atggtggtgg tacctcctat tacacggtca
     1861 ttatgcaatg caaccaattg gccttcagga aagatttcaa ggtgtttgag gtaatgtgct
     1921 ttatgcagga tttcatcttc agcaagatta ggaaatacta tgtattgcaa atcttccaat
     1981 gcttgcgcgt gctcagcacg ggtatgtatg atgatgatgt tcccacttat tatttcgtta
     2041 ataatctcca ttacaatttc tttacgccat tacatatccc aaaataatat atgccaacac
     2101 actcacaccc caagaggtga gtgcataagg caattgagat actccatgtt gcatattatt
     2161 tgtttcacaa ctctgcgatg tgagcactgt ggcatcggaa tacaaacatg cattggcgcc
     2221 aaacacaccg gcactgatta tagcgccgat attcaatagt acatttgaat caatactttg
     2281 cgcaagcgga attacaatag gaattgccac tacatataat ccccagcttg aaccggtagt
     2341 aaaggatatt aaactcaatg caataaaaat cgacaccggc aaaaatgcac tgctgacata
     2401 gggagtaaca ctatttatga catattgtgt aagtcccatt ttatcaccga cgtccttcaa
     2461 catcagcgac actacaacaa gtgccagtgc aaacaacatt gagttaaatc cgctgaaaat
     2521 tgtttctgtc agatgctgta cattcgacag cttcatatat agaaaataaa agaatgtaaa
     2581 tgctacacct atgatcacac cttttagcgc atcaatttca attccgggca ccgacggatc
     2641 ctgaaaggca actgtagcac ctaatacaac gatcataggt aataaaaaat atcgcgcctt
     2701 tggatgtacc agtcccggaa atgtttcaaa ttgcactgtc gacaatttaa taccaacagg
     2761 cacggttaac cctgtttcct cagcacgcgc aattgcttgc ttcatctttt tgaaatgagg
     2821 taaaattccg taaataaaca gcggtaccat gaatatagat atccatccgt aggcaacgaa
     2881 gggaataaca ctcatatatt tctgcagccc ttgaccttca gcggcaaatc cggaattttc
     2941 gagttgtacg ccaataaata atgtccacgt tgaaatcggc acgataacac ataatggtgg
     3001 cgataaggta tttacaatat atgccagata ttctctcggg attttaaatt tatcggtaac
     3061 agcacgcatt gtaataccag tggtaagagc gctgagatag tcgtcgatga aaacaaaaat
     3121 gcccagcaac catgtgccca ttaatgcctg acgttttgtt ttcatgtatc gcaacatgcg
     3181 ttcaccaaat tgcatggcgc caccactgcg aatcatcatt gccatgaccg agccatacaa
     3241 tccacataca acaataaccc agatggtgtc tgttgattgc atgacttcgt acaatgaggt
     3301 aatgaatcca tcaaaaaatc cccagccgtc gacaataata aatcctgtta cgcaaccgat
     3361 gagcaaacct tcgaatgatg atttgagcat gatggaaacg atgatcacca ctaaaggtgg
     3421 tatcagtgcc agccatccgt aatctgttgc caggtgttcc atttcaggtg tgtttttgca
     3481 ttttttcagt ttagactgct attggtgtag tctagacttc atagtccgcc gatactgatt
     3541 aatttctttt ccatgtattc caacaagcct tctctaccgc tttcatgacc taaaccgctt
     3601 tgtttccagc caccaaatgg cgcttcagta gcatgagcgg cccattcatt tatgccgaca
     3661 ataccaaatt ccaatgcttc actgatatag gtggcgtgat tgatattgtt cgtccataca
     3721 taagctgcca atccataagg tgtttcattt gccgatttaa ttacctcatc gatatcattg
     3781 aatggaatca acggcataac cggaccaaat atctctcgat cataaaatac ggatgctaca
     3841 ttttttacgc gaattacagt tggcgcaaca aaatgtccgc ctgctatgct atgacctcct
     3901 gtcaatactt catttccttc cgctttagcc tcggtgatta ttgacaaaac atgatcttgt
     3961 tgtatgctat tgatcaatgg tccgatgcgc gtttccggct caagaccgtg accaacattt
     4021 aattgcgcgg tatagtagac aacgcgttcc tgaaaggcgt caattatatc ggcatgtaca
     4081 aaaaatcgtt gtggtgcgat gcatacttgt ccgcaatttc taaatctacc tgccacagct
     4141 gtttttgcaa catgatctat atcgacatca ggcataataa ttaccggagc gttgcctcca
     4201 agttcgaggg caagtttcgt atttgtttcg gcagcaccac tcattaatat tttgcctacg
     4261 cgagtacttc ctgtaaaact aatttttcgc acacgtgcat cttgcaataa tatttttcct
     4321 gtattagcag catcgccatt tacaagattg acaacgccat ttggaattcc cgatgcttcg
     4381 agcgcattta ttaagagcat gccggaaaga ggtgtaaact cgctcacttt agcaactatc
     4441 gtgcagccgg cagctaatgc agcagcccag gcacgggcag gattataagc agggaaattc
     4501 caggcagtaa tgacaccgat aacacccata ggctgcagaa ttacctgcat gcgtttatct
     4561 gttcttgccg atggaattac agtgccataa gcacgtttgc cttcttcggc ataccattcg
     4621 aataaatttg cggcaacggt ccattcgcct ttcgcttcag ccagcggttt accactttct
     4681 ctgaccatta aagttgcaaa ttcaacaata tgttggcgga tataattcgc tgttttttgc
     4741 aggatatcag ctcttgtcca tggcgacatt ttcttccatg ttctaaatgc atcatgtgcc
     4801 gcatcaatgg caattgtagt atcattgcca tcgccgtaag gcacagtttg cacatactca
     4861 ccattagcgg gatctattac ttgttgcgtg cctgcatttg cagcatcaca ccaattccca
     4921 tttatatatt gtttaaactg catgcgtgaa attatttaca ataataggca acagcttctt
     4981 ccaaaatatc taatccactc tcgagttgtt catcagtaat taccagaggg gcgaggttgc
     5041 ggatcacatt tccatgtatg ccggagctga ttgcaatcac accattttcg taacagcgtt
     5101 gcgtgatttt tttacagaga tcaccattgg gtttgcggat atcgccatca tacgataatt
     5161 cgaatgccat catggcaccc attccacgca cactggaaat atgttgcgga taattatcac
     5221 gcaattgcat aaatcgcttt ctgatgatgc tgcccacgtg catgcctttt gcattaatat
     5281 cgatttcctt catataattt atggtagcgc ttacggcaac catgcataaa ggattaccca
     5341 ggtaagtacc accaattgtt ccggcaattg cggcatccat gatatgggct tttcccatca
     5401 cagcaccaac cggcattcca cttcccatac ttttcgccca ggttgagata tctggtatta
     5461 cacccaattg ttgatacgcg ccccaagaac ctgtgcgacc aaaacctgtt tgcacctcat
     5521 ccaatatgag cacaatacca tgttcattgc agaggtttct tagctgctgt aaataatgtt
     5581 tcggcaccac atgaaatcca ccttcacctt gaacaggttc aagaataatg gcagccactt
     5641 gtttagcagg aatatgcgta ttaaaaaacg agcgcaaatt ttcgatatgt aaatcgctga
     5701 atgaatcttc atccaatggc ccctcatatg ctgcaaatgc cggaaaatca atgcgataaa
     5761 tttcaggcgc ataaggacca aatccggttt tataacccac cttacttgtg agtgtggcag
     5821 ccattagcgt tcttccatga aatgcctggg tataagcaat cactgcagat cttcctgtag
     5881 cctgacgtgc gattttaatt gcattctcaa ttgcttccgc accgctgttc gtgaacatga
     5941 tttttgtagc atcgccatga ggcaatatgt cgatcatgtg ttgagccgca tcgagatatt
     6001 gctcataaat ggaaacatta aaacatgcat gaataaattt ctctgcctgc tgctgaatag
     6061 cacgcacaac aggttccgga caatggccgg cattcaaaac accgatacct cctgcaaagt
     6121 cgatcaacgc tcgaccatct gcatcataaa caatggcgcc tgaagcatgg gaaatactgg
     6181 atggattaaa aatccccaat gcattcggca ctaattgctt tcttcgctca tacagtgatg
     6241 ctgttttatt cattactact tcagtactca tgcaatataa atggttttaa tattcataaa
     6301 ttctttcatt ccgaaatgtg ataattctcg accatatcct gatcgcttaa taccaccaaa
     6361 aggaatgcgt ggatctgatt tcaccaatgc atttacaaac acacttcccg attcaatttg
     6421 tcgcgcgtaa tgttttgctt tatccatatc ttgtgtgaaa atcgaggccc ctagtccata
     6481 acgggaatca tttgccatgg ctattgcctc ttcggcatta gctgcggaaa tcacagctgc
     6541 aattggaccg aacaattctt catcaaatgc gggcattcct tttcccacat gatcgagcac
     6601 tgaaggcaaa aaatttgctc ctttgcgcgc gccgcctgca agcagtcccg caccttttcg
     6661 caaagaaatc tgcaattgcg actccaattg tgccgccaga tccatgcgtg ccattggccc
     6721 aattgtggta tcggcattga aaggatcacc ttgtttcatt gcggaaattc cattgatgag
     6781 tgcctgaata aaatcgggtc tgattttttc ctccacaata aaacgcttcg atgcgat
//
LOCUS       DAOQOU010000172         6798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6393_length_6798_cov_4.891888, whole genome shotgun sequence.
ACCESSION   DAOQOU010000172 DAOQOU010000000
VERSION     DAOQOU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6393_length_6798_cov_4.891888"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLM90_12305"
     CDS             <1..244
                     /locus_tag="PLM90_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90209.1"
                     /translation="LCFFNVELLAWNVPFSPIIYEAIQDISITLFLIITLFMMIGMIA
                     INMILMYFSINEIESAGALQQRILTLKKNKKEFSKA"
     gene            241..894
                     /locus_tag="PLM90_12310"
     CDS             241..894
                     /locus_tag="PLM90_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90210.1"
                     /translation="MRWLLILLLSISWQFSFAGDQPVYHPIDKETWKKVTDGVSYYEE
                     EEKVETTPEDFQVPTPAKPISVSPIVQIIGIVLIAALLAFIIWKLFGNIFTGNKKVDA
                     VIINSDNDLDDRPMETDLERYLREALEGGDFRMAVRIYYLMLLKALHDGGKIEWRKEK
                     TNRDYLIELYQHPQFHRLNNSTLVFEYVWYGECKVDSNTFSAIKPGFVDLIQKVSQP"
     gene            891..2228
                     /locus_tag="PLM90_12315"
     CDS             891..2228
                     /locus_tag="PLM90_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90211.1"
                     /translation="MNRSNVIIISVALALFIGILVFLFASNGETHFNWNPDYQSDSDE
                     PYGTLIFSELTEKYFPGQEREIVTTNKIKDVLNNPGTEHKIYMFIGESVFYSDSTIES
                     LLNFVSSGNEAFFAVDEIPIILSQRVIRGNSDYKIDSTYYIDTFGDTVSIPLEDEYSL
                     VPEDDKLTGSFITTGMTLNYNDSVFRSSNGYRFVFRVKEKPYDFEWRYFMPEYLQGNA
                     FTDNLGTIQSRNFCNFIRMPYGDGYFYFHLTPIAFTNLFMLEEQKVEYASKVLSYFEP
                     GDIIWDQYSMSFKQKKNNDDQEEGPLKFILSQTSLRWAWYILLSGMGIFLIFRTKRMQ
                     QSIPVMEPNENKSLEFVQTIGRMYLLRNKHHYLVTQKMKLFNHFIQERYQLHTAQYDS
                     AFMSKLQMKSEIPIEDIEGIYALANKLEAKHSVTADELILLHNTLDKFYKHCK"
     gene            2234..3217
                     /locus_tag="PLM90_12320"
     CDS             2234..3217
                     /locus_tag="PLM90_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ90212.1"
                     /translation="MEENFVFQETNPEIQQLQQAVYAVRAELSKVIIGQQQMMDLLIA
                     GLLAGGHILIEGVPGIAKTLTAKMLAQTVAADFSRIQFTPDLMPTDVLGTSVFNQKTT
                     EFNFRKGPIFSNIVLIDEINRSPAKTQAALLEVMEELKVTMDGETYPMTFPFFVIATQ
                     NPIEQEGTYKLPEAQLDRFMFRIKITYPDLDQEKQILYRFKEDFTNRQKDSVQRVMSV
                     DDLKRYRNTVEHIHIKNELLDYIAQIVFKTRNHGDLFLGASPRASINIMKASKAVAAM
                     NGRNFVTPDDIHFVAYAVLNHRIILTPDKELEGYDSAGVIKTIIESIDVPR"
     gene            3217..4548
                     /locus_tag="PLM90_12325"
     CDS             3217..4548
                     /locus_tag="PLM90_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ90213.1"
                     /translation="MRPFSSIYVTNRLFWAIGAVATLFAISFSVSILFYVAQAALALL
                     VIIVLVDAMLLYIPSTKVSSVRSTGNVLSLSDDNMVHIRIRNHSAIPLTVQVIDELPY
                     QLQKRDFDVTLHLASGEKKSIDYPVRPVIRGAYTFRNINMFATSVIGILQRRFVTRTP
                     LTVPVYPSLIQMRQYELKTMSNISFFEGVKKLRRIGHSYEFDQIKQYVRGDDIRHINW
                     KATGRTGQLMVNHYEEEKSQQVFCIIDKSRTMKMPFHDLSLLDYAINTTLTISNIAMR
                     KDDRTGLITFSDKIGTTLSPDKHHAQLRKIMEALYNEKERFTEANYELLYLHVRNFIR
                     VRSLIFLFTNFESLYAIERVVSILRKLNKLHLLVVIFFENAEISNYAREDASNVEEIY
                     LTTIADKFAVEKKQIQRELTKYGIQTIISRPEDLSINTINKYLELKSRGLI"
     gene            4673..5377
                     /locus_tag="PLM90_12330"
     CDS             4673..5377
                     /locus_tag="PLM90_12330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ90214.1"
                     /translation="MNKLILIPVAVMMLATVISCESTQKVQNLKLENASDSLSYAIGY
                     NIGQSFKEQKLTEVDPQLMAVVINAILADDTAGLKMDGTTSVTFIQGYMQKKQEMEDA
                     KAKEEGIKYLEENAKKSGVKVTPSGVQYEVITAGNGKTPVDGDNVTIHYTGTFVDGKE
                     FDSSVRNGQPVTYPVNGFTPGFSEALKMMKEGDKWRVVIPSDLAYGPAGFQGVIPPNA
                     VLIFELELLKVSPATK"
     gene            5525..>6798
                     /locus_tag="PLM90_12335"
     CDS             5525..>6798
                     /locus_tag="PLM90_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ90215.1"
                     /translation="MRIFTLSLLVAGVITLGQAQPAESGAYSIAISEFNYNSDKFNNS
                     SDWVELHNYGGTAQDLSNWKLKDAIGTTFTIPGGTSLSAGSYLVLVERTDTFAMIHPT
                     VSNYIGNLGFGLDNNSGSIRLFDAGGAIVKQINYVDSIPWPKGSDGYGPSVQILDELA
                     SETDGTNWIAGCILGTPGSAYVPCDYDIVVSEINYNSLPAYNPGDWIELYNRSTSNIG
                     IGGWTIRDSRNANAFLIPNGTTLNAGARLVITDSLESFNILFAGVTNVIGEPTFNFSN
                     GGDAIRIYEPGDKLKYSIRYKDSAPWPLDPDGTGFTLELVDDSGNPNVAASWVAGCLY
                     GSPGTPFIFPCPDAVTNMHPSDIVVGPVPFTSGLHISLASGNAIDQYKVIDLQGNVVF
                     TQTANAQFAIWDGTNANGTEVPAGIYVISIID"
BASE COUNT         2077 a         1336 c         1429 g         1956 t
ORIGIN      
        1 tttatgcttt tttaatgttg aactgcttgc ctggaatgtg ccatttagtc cgatcatata
       61 tgaagcgata caagatatca gcatcacctt atttttaatt attacattat ttatgatgat
      121 aggcatgatt gccatcaaca tgattttaat gtatttcagc attaacgaaa tagaatccgc
      181 aggtgccttg caacaacgca ttttaaccct gaaaaaaaac aaaaaagaat ttagtaaggc
      241 atgaggtggt tgttgatatt attgctttca atttcatggc agttttcatt cgccggagat
      301 caacctgtat accatcctat tgataaggaa acatggaaaa aagtgacaga tggtgtcagc
      361 tattacgaag aggaagaaaa agttgagaca acgccggaag attttcaagt ccctaccccg
      421 gccaaaccca ttagtgtcag tcccatcgtg caaataatcg gcattgtgtt gattgcggca
      481 ttactggcat ttatcatctg gaagcttttc ggtaatattt tcacaggaaa taaaaaagta
      541 gatgctgtta ttattaacag cgataatgat ctcgacgaca ggcctatgga gactgacctc
      601 gaacgctatt tgcgagaagc attggaggga ggtgattttc ggatggctgt gcgcatttat
      661 tatctcatgt tgttgaaggc tttacatgat ggcgggaaaa ttgaatggcg caaggaaaaa
      721 acgaacagag attatcttat cgaattatat caacatccac aattccaccg actgaacaat
      781 tcaacgctgg tatttgaata tgtgtggtat ggtgagtgta aagtagattc aaacacgttc
      841 agtgcaatta aacccggttt tgtcgacctc attcaaaaag tcagccagcc atgaacaggt
      901 cgaatgtgat tataatcagc gttgcacttg ccctatttat aggaatattg gtgtttctgt
      961 ttgcctctaa cggggagacg catttcaatt ggaatcccga ctaccaatca gactccgatg
     1021 aaccatacgg caccctaatt ttttctgagc ttaccgaaaa atattttccg ggtcaggaaa
     1081 gagaaattgt gacaacgaat aaaataaagg atgtgctgaa caatcccggc acagaacata
     1141 aaatctacat gtttattggg gaatctgttt tctattccga cagcactatt gaatcattat
     1201 taaatttcgt ttcatccgga aatgaagcgt ttttcgccgt ggatgaaatt cccattattt
     1261 tatcgcaacg tgttattcga ggtaacagcg actacaaaat agacagcact tactacatag
     1321 atactttcgg cgacaccgtt tcaattccgc tggaagacga atatagcctt gtgcctgaag
     1381 acgataagct gacaggcagc tttatcacta caggtatgac tttgaattac aatgattctg
     1441 ttttccgttc atcgaatggc tatcgatttg tatttcgcgt gaaagaaaaa ccatatgatt
     1501 tcgaatggcg atattttatg ccggaatatt tgcaaggcaa tgcatttacc gacaatcttg
     1561 gcaccattca gagtcgcaat ttctgcaatt tcatccgcat gccctatggc gatggatatt
     1621 tctactttca tctgactccg atcgcattta ccaacttatt catgctcgag gaacaaaaag
     1681 tagaatatgc atctaaggta ttgagttatt ttgagcccgg tgatattatt tgggatcagt
     1741 attccatgag tttcaaacag aaaaaaaata atgacgatca ggaagaaggc cctttgaaat
     1801 tcatattaag ccaaacaagc ctgcggtggg catggtatat cctgctttca ggaatgggta
     1861 tttttcttat tttcaggaca aagcggatgc aacaatccat tcctgttatg gagcctaatg
     1921 aaaataaatc attagagttt gttcagacta tcggaaggat gtatctgttg agaaataaac
     1981 atcattatct ggtaacccag aaaatgaaat tattcaatca tttcatacag gaaagatacc
     2041 aattgcatac cgctcaatac gacagcgcat ttatgtcgaa actgcaaatg aaatcggaga
     2101 ttccgattga agatattgaa ggcatatatg cattggcaaa taaattggaa gcaaaacact
     2161 cggtaactgc cgatgaattg atattattac ataacacact tgataaattt tataaacatt
     2221 gtaaataagc aatatggaag agaatttcgt atttcaggaa accaatccgg aaattcaaca
     2281 attacaacaa gcggtttatg ctgttcgcgc agaactgtcg aaggtgatca tcggacaaca
     2341 acaaatgatg gatctgttga ttgcaggatt attagccgga ggccatatat tgattgaagg
     2401 tgttcccggt attgctaaaa cgctgacagc taaaatgttg gcgcaaacgg tggcagcaga
     2461 tttttcgcgc atccaattca cccccgacct gatgcctaca gatgtattgg gaaccagtgt
     2521 tttcaatcag aaaaccacgg aatttaattt caggaaaggt ccgatttttt cgaatattgt
     2581 gttgattgat gagatcaacc gttcgcctgc aaaaacacaa gctgccttgc ttgaggtgat
     2641 ggaagaatta aaggtgacca tggatggcga aacatatccg atgacctttc cattctttgt
     2701 cattgcaaca cagaacccca tcgagcagga aggaacctac aaacttccgg aagcgcaatt
     2761 agaccgcttt atgttccgca ttaaaattac ctatccggat ttagatcagg agaagcaaat
     2821 tttatatcgc tttaaagaag atttcactaa cagacaaaaa gatagtgttc aacgcgtaat
     2881 gagtgttgat gacttgaaac gctatcgcaa cacagttgaa catatccata ttaagaacga
     2941 gttattggat tatatcgcac agattgtatt caaaacacgc aaccacggcg atcttttcct
     3001 gggcgcctct cctcgtgctt caataaatat tatgaaggcc tcaaaagcag ttgcagcaat
     3061 gaatggcaga aactttgtga cacctgatga tattcatttc gttgcttatg ccgtattgaa
     3121 tcatcgtatt attcttacac cggataaaga actggaaggt tatgacagcg ccggcgtgat
     3181 taaaacaatt attgaaagta tagatgttcc acgttaatgc ggccattcag tagcatatat
     3241 gtaactaaca gattgttttg ggccattggc gcagtagcaa cattatttgc catcagtttt
     3301 tcggtgagta tacttttcta tgttgcacaa gcggctctgg cattattggt cattattgtt
     3361 ttggttgatg caatgttgct ttacatccca tcaacaaaag tcagttcagt tcgcagcacc
     3421 ggcaatgtat tgtcgctgag cgatgacaat atggtgcata ttcgcattcg caaccacagt
     3481 gccattccgt taacggttca ggtaattgat gaactcccct atcaattaca aaagcgcgat
     3541 tttgatgtga ccttacatct tgcatcgggc gaaaagaaaa gtattgacta tcctgtaaga
     3601 ccggttattc gaggagcata cacgttcagg aatatcaaca tgtttgcaac ctctgtcatt
     3661 ggtattttac aaagaagatt tgttacgcgc actccactga cggttcctgt gtatccttca
     3721 ctgatacaaa tgcgtcagta tgagctgaag accatgagta atatcagctt ctttgaaggc
     3781 gtgaaaaaac ttcgccgcat tggtcacagc tatgagtttg atcaaatcaa acaatatgtt
     3841 cgcggcgacg atatcaggca tatcaactgg aaggcaacag gcagaaccgg gcaattaatg
     3901 gttaatcatt acgaagagga aaaatcgcag caagtgttct gcattattga taagagcaga
     3961 acgatgaaga tgccttttca cgatttgagt ttattggatt atgccatcaa caccaccctt
     4021 accatcagca atattgcgat gcgcaaagat gacagaacag gcttgattac tttttctgac
     4081 aaaatcggta ctaccctatc gcccgataaa catcacgcac aactgcgcaa gattatggaa
     4141 gcgctgtata atgaaaaaga gcgtttcact gaggcgaatt atgaattgtt atatctacac
     4201 gtgcgaaatt tcattcgcgt acgcagttta atattcctat ttacaaattt tgaaagttta
     4261 tatgcgattg agcgcgttgt atccattttg cgtaagctga ataagttaca cttattggta
     4321 gtgatcttct ttgagaatgc cgaaatatcg aattatgcca gagaagatgc atcgaatgta
     4381 gaggaaattt acctcaccac tatagcagat aaatttgccg ttgagaagaa gcaaattcaa
     4441 cgcgaactaa cgaagtatgg cattcaaacc attatttcac gccctgaaga cttaagtatc
     4501 aacaccatca ataagtacct cgaattgaag tcgcgcgggc tgatctagca ctcatttcta
     4561 acaagtttta acgtttttct acccttctgc gggcgttttc aagggctatt tcagggtcag
     4621 ggccacaaag gctcatgcaa tatccctatc tttgcggcct caaaatcaca atatgaacaa
     4681 attaatcttg attcccgtag ccgtgatgat gttggcaacg gtgatctcct gcgaaagcac
     4741 tcaaaaggtt cagaacctca aactggaaaa tgcatcagat tctctcagct atgccattgg
     4801 ttataacatt ggtcagagtt ttaaggagca aaaattgaca gaagtagatc cacagttaat
     4861 ggcagttgtg atcaacgcta ttttggctga tgacactgca ggcttgaaaa tggacggtac
     4921 cacctctgtt actttcattc aaggctatat gcagaaaaaa caagagatgg aagatgcgaa
     4981 ggctaaagaa gaaggcatca aataccttga ggaaaacgca aagaaatcag gtgttaaggt
     5041 tacccctagt ggagttcaat atgaagtgat tacagcaggt aacggtaaaa caccggttga
     5101 tggcgacaat gttactattc actataccgg cacctttgtt gacggcaagg aattcgatag
     5161 ttctgttcgc aacggtcagc ctgtaaccta tcctgtaaac ggtttcaccc ccggattcag
     5221 cgaagcattg aaaatgatga aggaaggcga caaatggcgt gtggtgatcc cttcagacct
     5281 cgcttatggt cctgcaggtt tccagggagt gatacctcca aatgccgtgc tcatttttga
     5341 attggaattg ttgaaggtta gccctgcaac caaataataa tgtcaaaaat cgtgacaaga
     5401 cccgcctgcc ttatggtatg cgggtttttt gttgctgtca aatcaaggtc aatttaaacc
     5461 cggagtcccc tccgaccgtc ctatcactta acaatttatt aattttaggg caatattacc
     5521 attcatgcgc atttttacat tgtctctcct cgtagccgga gttattactt taggtcaggc
     5581 acaacctgcg gaatccggtg cttacagcat tgccatctca gaattcaatt acaattccga
     5641 taagtttaat aattcatcag attgggttga attacacaat tatggtggca cagctcaaga
     5701 cctttctaat tggaaattaa aggatgctat aggcaccacg tttactattc ccggaggcac
     5761 cagcttatct gcgggaagct accttgtttt ggtggaaagg actgatacat ttgccatgat
     5821 acaccctacc gttagcaatt atattggtaa tcttggtttt ggactggaca ataacagcgg
     5881 aagcattcgc ttattcgacg caggtggcgc cattgtaaaa caaattaact atgtagattc
     5941 aattccatgg ccaaaaggtt ctgatggtta tggtcccagt gttcaaatat tggatgaatt
     6001 agcctcagaa acagacggca ccaattggat tgcaggctgc attctcggca cccccggaag
     6061 tgcttatgtg ccatgcgatt atgatatcgt tgtaagtgaa attaattata atagtttgcc
     6121 ggcatataac cccggcgact ggattgaatt atacaatcgc agcacatcta atatcggcat
     6181 aggtggttgg acgattcgcg attcccgaaa tgcgaatgcg tttctgatac caaacggaac
     6241 aactttaaat gcaggagcaa gacttgtaat tactgacagt cttgaatctt tcaatatttt
     6301 atttgccggt gtaacgaatg ttattggtga accgacattt aatttttcaa atggcggtga
     6361 tgcaataaga atttatgagc ccggagataa actgaaatac agtatacgtt acaaggatag
     6421 tgctccatgg ccactggatc ctgatggaac aggttttaca cttgaattgg ttgacgatag
     6481 cggaaatcca aacgtggcag catcgtgggt tgcaggttgt ttatatggat cgccgggaac
     6541 gcctttcatt ttcccttgtc cggatgcggt aacaaatatg catccgagtg atattgtagt
     6601 aggaccggtt cctttcactt caggactgca tatttcgctt gcttctggca atgcgataga
     6661 tcaatacaaa gtaattgacc ttcaaggtaa cgttgtattc actcaaacag caaatgcaca
     6721 atttgctatt tgggatggca ctaatgcaaa tggaacggaa gttcctgccg gcatttatgt
     6781 gatttctatt atagataa
//
LOCUS       DAOQOU010000173         6732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6491_length_6732_cov_5.991014, whole genome shotgun sequence.
ACCESSION   DAOQOU010000173 DAOQOU010000000
VERSION     DAOQOU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_6732_cov_5.991014"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..3731)
                     /gene="metH"
                     /locus_tag="PLM90_12340"
     CDS             complement(84..3731)
                     /gene="metH"
                     /locus_tag="PLM90_12340"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HMZ90216.1"
                     /translation="MEQKLRKILKERILVLDGAMGSMIQRYKLSEADFRGERFATHPH
                     DLKGNNDLLSITKPEVIREIHRQYLEAGADIIETNTFGATAIAQADYHLESLGYELNK
                     VSAEIARLEADAFTALNPEKPRFVAGAIGPTNKTLSLSPDVNNPGFRSVYFDAMAQAY
                     YDAACGLMDGGAHILIIETIFDTLNAKAALYAIDKLFNETGMSLPIMVSGTITDASGR
                     TLSGQTLEAFVISMSHLPLLSIGLNCALGAKQLKEHIEILSRETDLFVSAYPNAGLPN
                     AFGEYDETPESNGNDVEVYLQHGWVNIIGGCCGTTPDHIRYIAEKAKKYPPRVPPTLP
                     RLPKFSGLEKLVIYPESNFLNVGERTNITGSKQFKRLITEEKYDEALVVARQQVENGA
                     QMIDVNMDEGMLDSVEVMRTFLNLIAAEPDISKVPVMIDSSKWEVIEAGLKCIQGKGV
                     VNSISLKEGEEAFKHHAREARRLGAAVIVMAFDETGQATSFEHRIAICARAYKILTEE
                     VHFSPTDIIFDPNILTVGTGIEEHNNYAVDFIKATAWIKENLPGALVSGGVSNISFAF
                     QGNNTVREAMHSAFLYHAIKAGMDMGIVNAGMIDIYENIDAALLQHIEDVLLNRHPDA
                     TEKLISFAEQLKQKDKTAIQTDTWRSLPLEARIQHALVKGIADHIEADMEEALPAYPG
                     PLEIIEGPLMAGMQVVGDLFGSGKMFLPQVVKSARVMKKAVAYLQPYIEQTKLQNNQS
                     SNRKKILLATVKGDVHDIGKNIVGVVLACNNYDIIDLGVMVPAEKILQTAVQEHADII
                     GLSGLITPSLDEMVHVAQLMQERHMSTPLLIGGATTSRAHTAVKIAPAYQQAVVHVTD
                     ASRSVTIAGKLLGGEKAAFVKEIADEYAIVQQQHAARQSFKGYLTLAEARNNAFTLSE
                     AAHYPYQPVQPGIHVLDIAIDTLIPYIDWTPFFTTWELHGKYPMIFEDAVIGEEAKKL
                     FDDAQEMLQQIAHEKWLQSKAVIGLFACNRDGDDIVIEETQTRLHHLRQQNKKADGQP
                     NYCLSDFVLSANSGVKDYMGAFAVTAGIGIETKLAEFNAAHDDYKSILLKALADRLAE
                     ACAEYMHQQVRTKYWGYAANEQLTNEALISEQYQGIRPAPGYPACPDHTEKVSLFNLL
                     DVTKHTGVQLTESMAMYPAASVSGWYFAHPDAKYFGVGKIGKDQVEDLAKRKGKDFVE
                     MEKWLRPVVNY"
     gene            4226..6421
                     /locus_tag="PLM90_12345"
     CDS             4226..6421
                     /locus_tag="PLM90_12345"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ90217.1"
                     /translation="MLKKSLLSTAFAFVLGGLVMAQAPSRSCATMDVDARLRAEDPTY
                     AANREAIEAFTQNYIATEGERAVVTIPVVVHVVYNVAAENISDTRIFEQIDVLTKDFR
                     RLNADVGETPAYFAGVAADCEVSFCLATVDPSGNPTTGITRTSTTKTSFSTADDMKFA
                     TYGHVAWDRNKYLNLWVCDLGSGLLGYAQFPGGAAATDGVVIDYAYFGVTGAGAPYNE
                     GRTATHEVGHWLNLYHIWGDDGTGCGGSDSVADTPNQADENYGCPGGTIRISCSNGPN
                     GDMYQNYMDYTDDGCMNLFTAGQKTRIQALFAVGGSRVSLTTSNGCGAAATCSTPTGM
                     STTSIGTTSATFNWTAVAGASSYNVQYRIVGAATWTSTTSATNSKAVTGLTAGSNYEW
                     QVQTVCAGGTSSWTASTNFSTTGGATCAVPTGMNTTSIGTTSATFNWSAAAGATSYNV
                     RYKATAAATWTTTTSATTSKAVTGLTAGTAYEWQVQTVCSGGTTSAYTTSTNFTTTGG
                     ATCVDNYEPNNSRGTAALVSTGVTYGELIATSTDIDWFKFTTTSPNTKIKVNMTSLPA
                     DYDIRLYDNAGTQKGSSINGGTTDEQIIWNSTSAGTRYIKINGYAGAFNASDCYDFLI
                     SVASTNWKTDEAFADVAGKEWENTIMGIYPNPVTGSVVTVDYFSVLEDMDVTVQVFDV
                     LGRMVSSSVVGIVNGPNSIDLNVADLQSGMYFVTISSAKFMQSQNFIVE"
BASE COUNT         1759 a         1592 c         1351 g         2030 t
ORIGIN      
        1 ttccttcttt aaaactaaca ttctagatga aaaagacctg tttaaggtct aaaggcctct
       61 gcgcctctgc gtgacacccg ccgttagtaa ttcaccaccg gtctcaacca tttttccatc
      121 tccacaaaat ctttcccctt ccgcttggcc agatcctcaa cctgatcttt gccaattttt
      181 cccacaccaa aatacttcgc atccggatgc gcaaaatacc atccgctcac actcgctgca
      241 ggatacatag ccatgctttc tgtcaattga actccggtgt gtttggttac atccaacaaa
      301 ttaaacaagc taaccttctc cgtatgatcc ggacaggcag gataaccggg ggcaggacgt
      361 atgccctgat attgttcgct gatgagcgcc tcattagtta attgctcatt cgctgcatat
      421 ccccaatact tagtgcgcac ctgctgatgc atatattctg cacatgcttc cgccaacctg
      481 tcggctaatg cttttaataa aatgcttttg taatcatcat gtgcagcgtt aaattcagcc
      541 agcttcgttt caattccaat acctgctgta acggcaaatg cacccatgta atcttttacg
      601 ccgctgtttg cacttaatac aaaatcactc aaacaataat ttggctgacc atcggctttt
      661 ttattctgct gtcgtaggtg gtgcaatctg gtttgtgttt cttcgatcac aatatcatca
      721 ccatctctat tacatgcaaa taatccgatc actgctttcg attgcaacca tttttcatgc
      781 gcgatctgtt gcaacatttc ctgtgcatca tcaaacaatt tcttcgcttc ctcgccaata
      841 accgcatctt caaatatcat aggatatttt ccatgcaatt cccatgttgt aaaaaatggg
      901 gtccagtcga tatacgggat cagcgtatca attgcaatat ccaaaacatg tataccgggc
      961 tgaacaggct gataaggata atgcgctgct tcagataaag taaatgcatt attccgcgct
     1021 tctgccagag ttaaatatcc tttaaatgat tgtcgcgctg catgttgctg ctgcacgatc
     1081 gcatactcat cagcgatctc tttaacgaat gcagcttttt ctccgcccaa taatttcccc
     1141 gcaatcgtaa cactgcgtga agcatcggtt acatgcacaa cagcttgctg gtatgccgga
     1201 gcaattttta cagccgtatg tgcccgcgaa gtagtggcac caccaatcaa taatggtgtt
     1261 gacatatgcc gctcctgcat cagttgcgct acatgcacca tctcatctaa tgatggtgta
     1321 attaatccgc tcaatcctat gatgtctgca tgctcctgca ctgctgtctg caatattttc
     1381 tcggcaggca ccatcacccc caggtcgata atgtcgtaat tgttacaagc caagacaaca
     1441 cccacaatat tttttccgat atcatgcaca tcacctttca ccgttgccag caatattttc
     1501 tttctgttgc tgctttgatt gttttgcaat tttgtttgtt caatatatgg ctgcaaatag
     1561 gctactgctt ttttcattac acgcgcgctt ttcaccacct gcggcagaaa catttttccg
     1621 ctgccaaaaa gatcgcctac tacctgcatg ccggccatga gcggaccttc aatgatctcc
     1681 agtggtccgg gatatgcggg caatgcctct tccatatctg cttcaatatg atcggcgatg
     1741 cctttcacta aggcgtgttg tatgcgtgct tcaaggggca agctgcgcca ggtatctgtt
     1801 tgaattgctg ttttatcttt ttgctttaac tgttctgcaa agctgatcag tttttctgtt
     1861 gcatccggat ggcgattcaa taatacatct tcaatatgtt gtaataatgc agcatcaata
     1921 ttttcataaa tatctatcat gcctgcattc acaatgccca tatccatacc tgctttgatg
     1981 gcgtggtata aaaatgcgct gtgcatggct tcgcgaacgg tattattgcc ctgaaatgca
     2041 aaagatatat tacttacgcc accgctcact aaagcacccg gcagattttc tttgatccat
     2101 gcggtcgctt taataaaatc aacagcataa ttattgtgtt cttcaatgcc tgtgcctacc
     2161 gtcaaaatat tcggatcgaa aatgatatcg gtaggggaga agtgtacttc ttctgtcagt
     2221 attttatagg cacgtgcaca aatagcaatg cgatgttcga agctggtcgc ctgacctgtt
     2281 tcatcaaatg ccatcacgat cactgctgca cctaatctgc gtgcctcgcg tgcatgatgt
     2341 ttaaatgctt cctcaccttc tttcagagaa atggaattta ctaccccttt tccctgaata
     2401 cattttaatc ccgcttcgat cacttcccat ttactggaat ctatcatcac cggtactttc
     2461 gaaatatccg gttctgctgc gatgagattc aggaaagtgc gcattacttc aacagagtcg
     2521 agcatccctt catccatatt cacatcgatc atctgcgcgc cattttctac ttgttgacgc
     2581 gcaaccacta atgcttcatc atatttctct tccgttatta atcgcttgaa ttgtttactg
     2641 cctgtaatat tggtgcgctc acccacattg aggaaattgc tttcaggata gatcactaat
     2701 ttttccagac ctgaaaattt cggtaatcgt ggaagtgtgg gcggtacgcg cggcggatat
     2761 ttttttgctt tttctgcgat atagcgaata tgatcaggcg tggtgccgca acatccgcca
     2821 ataatattta cccagccatg ttgcagatat acttctacat cgttgccatt gctttcaggt
     2881 gtttcatcat attcaccaaa agcattaggt aaccctgcat tcggataagc agatacaaac
     2941 aaatctgttt cgcgggataa tatttcgata tgttctttta attgtttcgc accgagtgca
     3001 caattcaacc cgatactcaa caaaggcaaa tgactcatag aaataacaaa tgcttcgagt
     3061 gtttgtccgc tcaacgtgcg accactggca tctgttatgg ttccgcttac cattattgga
     3121 agcgacattc ctgtttcgtt aaataattta tcgatagcat ataatgcagc ttttgcattt
     3181 aatgtatcga aaatagtttc aatgatgagg atatgtgcac cgccatccat taatccgcat
     3241 gcggcatcat aatatgcttg tgccattgca tcgaaataca ccgagcggaa tcctggatta
     3301 ttgacatccg gtgaaaggct gagcgtttta ttggtgggtc cgatagctcc ggcaacaaac
     3361 cgcggttttt caggattgag ggctgtaaat gcatctgcct ccagtctggc aatttcggca
     3421 cttactttat tgagctcata tcccagggat tccaggtgat aatcagcctg agcgattgca
     3481 gtagccccaa aagtgttggt ttcaataata tcggctcctg cttcaaggta ttggcgatgt
     3541 atctcgcgga tcacctcggg tttggtaatg gacagcaggt cgttattccc tttgaggtcg
     3601 tgcggatgag tggcaaatcg ttcgcccctg aaatcggcct ccgacagctt atagcgctga
     3661 atcatgctcc ccatagcgcc atcaagcact aaaatgcgtt ccttgagtat cttcctaagc
     3721 ttttgttcca cagcgcaaaa ataggtgaac acagattaga gccgtttagt tttgagaaaa
     3781 ttgacaattc aatcgcaaac ggaacgtttg gttgcatttt atgcaaaacc gaaacataag
     3841 gctttcagcg atttgaagac tttgtaaata ctgctgcatt ttggctccgg cgtgacaggc
     3901 aaaaaaaatt atttataaac taaattcatt gataatcaat taattagaca attaaatgac
     3961 tgaaatttcg acacatttaa gcattcctgt gacattgaac agttccttgt ttataaaaag
     4021 gaagaaaaaa acttgttgga attaaaaatc ttgtaacttt acgcaacttt tttgggggag
     4081 ggggcgtctt gttcttcgat atagcaatat attgattttg tgctcatcgc gtttttctcc
     4141 tccgacgtga gcctagaaac accacacacc aaacagtatt atttatttaa cgtattgatt
     4201 tattaaccct aactattcat ttcacatgtt gaaaaaatcg ttactttcta cggccttcgc
     4261 ttttgtgctt ggaggtctgg tgatggctca ggctccatca agaagctgcg ccactatgga
     4321 tgtggatgca cgccttcgtg ctgaagaccc aacctacgct gcaaatcgcg aagcgatcga
     4381 agcatttact caaaactata tcgccaccga aggtgagcgc gctgtagtta ctattcctgt
     4441 tgtagtacac gttgtttata acgttgctgc tgaaaacatt tctgacacac gcattttcga
     4501 acaaattgat gtattgacaa aagacttccg tcgcctcaat gctgacgttg gtgaaactcc
     4561 tgcctatttt gccggtgttg ctgccgattg cgaagtaagc ttttgtcttg ctactgtaga
     4621 cccaagtggt aaccctacta ccggtattac acgcactagc actaccaaaa cttcattcag
     4681 cactgctgat gatatgaaat ttgctactta cggacacgtt gcttgggatc gcaacaaata
     4741 cctcaacttg tgggtatgcg accttggaag cggtttgtta ggttacgctc agttccccgg
     4801 tggagctgct gctactgacg gtgtagttat tgactacgct tacttcggtg ttacaggtgc
     4861 aggtgctcct tataacgaag gtcgtactgc tacccacgaa gttggtcact ggttaaatct
     4921 ttaccacatt tggggtgacg atggtaccgg ttgcggtggt tcagactcag ttgccgatac
     4981 tccaaaccaa gctgatgaga actatggctg tcccggtgga actatccgta tctcttgctc
     5041 taacggtcct aacggcgaca tgtatcaaaa ctacatggac tacactgacg atggttgtat
     5101 gaacctcttc actgcaggac aaaaaactcg tattcaggca ttgtttgctg ttggtggttc
     5161 acgcgtgagc ctcacaactt ctaatggttg cggtgccgcg gctacttgta gcactcctac
     5221 aggcatgtct accacatcaa ttggtactac ttcagctacc ttcaactgga ctgctgttgc
     5281 cggagcttct agctacaatg tacaatatcg tatcgttggt gctgccactt ggacttctac
     5341 aacttcagct acaaactcta aagctgtaac aggtcttact gcaggttcta actacgaatg
     5401 gcaagtacaa acagtttgtg caggtggcac cagctcttgg actgcttcta caaacttctc
     5461 aactacaggt ggtgcaactt gtgctgttcc aaccggtatg aatactactt ctattggcac
     5521 tacttcagct acctttaact ggtctgctgc ggcaggtgct accagctata atgttcgcta
     5581 taaagcaact gcagctgcta cctggactac gactacttca gctaccactt ctaaagctgt
     5641 taccggcctc actgcaggaa ctgcttacga atggcaagta caaactgtat gtagcggtgg
     5701 aacaacttca gcttatacaa catcaaccaa cttcactaca acaggtggtg ctacttgcgt
     5761 tgataactac gaaccaaata atagcagagg tacagcagct ctcgtatcaa caggcgtaac
     5821 ttatggcgaa ttgattgcta ccagcactga tatcgactgg ttcaaattca ctaccacttc
     5881 acctaatact aaaattaaag tgaatatgac ttcattacct gctgactacg atattcgtct
     5941 ttacgacaat gccggaactc agaaaggttc ttcaatcaat ggtggaacaa cagatgaaca
     6001 aattatatgg aactcaactt ctgcaggcac tcgctatatt aaaattaacg gctatgccgg
     6061 agcattcaat gcctcagatt gctatgattt cttaatttct gttgcttcaa ctaactggaa
     6121 aactgatgaa gcttttgcag atgttgcagg taaagaatgg gaaaatacta tcatgggtat
     6181 ctatcctaac cctgtaacag gttctgtagt tactgttgac tacttcagcg tgttggaaga
     6241 tatggatgtt accgttcaag tattcgacgt gctcggccgc atggtgagct cttcagtagt
     6301 tggtatcgtt aatggtccta actctatcga cctcaatgtt gctgatcttc aatcaggtat
     6361 gtatttcgta acgatcagct ctgcgaaatt catgcaatca caaaacttca ttgtagaata
     6421 atacatttct gcaatcaacc tataaaagag gctgtcttat taaggcagcc tctttttttg
     6481 gtgagtggtg agtgggtcag tgggtcagtg gggcaatttc acgactcacg actcacgact
     6541 cacgattcac gactcacgat attagtatca agaaccaagt atctagtagt aagactaatg
     6601 cagtggaatg tgccgtgaat tttctctgta aacctctgtg ttccgctccg tgacctctgt
     6661 ggttaaatgg tgagtggggg agtgaggcaa tttcacgatt cacgattcac gattcacgag
     6721 tcacgactca cc
//
LOCUS       DAOQOU010000174         6631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6631_length_6631_cov_5.786192, whole genome shotgun sequence.
ACCESSION   DAOQOU010000174 DAOQOU010000000
VERSION     DAOQOU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6631_length_6631_cov_5.786192"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..560
                     /locus_tag="PLM90_12350"
     CDS             189..560
                     /locus_tag="PLM90_12350"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HMZ90218.1"
                     /translation="MNAEFFLWVNAVLYAGFAAWCAISPTSTAAFSGFSFMNVNGRSE
                     YFALYVGLQAAWSVMFAICAINNDLQYAGVFYAVFLYAGVIFGRWVSIFQKGVAGNNV
                     YIIAILEIILGAWAVLLLSNF"
     regulatory      659..757
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1173..2486
                     /locus_tag="PLM90_12355"
     CDS             1173..2486
                     /locus_tag="PLM90_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HMZ90219.1"
                     /translation="MSNYQYETLQLHAGQQADPVTGSRAVPIYQTTSYVFNDSAHAAD
                     LFGLKAFGNIYTRIMNPTTDVFEKRIAALEGGVAALAVASGQAAQTIAITTIAQAGDN
                     IVSTSFLYGGTYNQFKVAFKRLGIDVRFAEGDDIEAFEALIDSRTKGIYLETIGNPGF
                     NIPDLEKFTALAKRHDIPLIVDNTFGAAGYIAQPIKYGANIVIASATKWIGGHGTSIG
                     GVIVDAGNFNWGNGKFPLFTEASEGYHGLKFWDTFGAGSPFGNIAFIIRARVEGLRDL
                     GPAISPFNAFLLLQGLETLSLRVQRHCDNAQQLAEWLEQHPLVEYVNYPGLTSSPYHA
                     LADQYLTNGYGGVLSFKVHGGTTNANKFVDALKLVSHLANVGDAKSLIIHPASTTHEQ
                     LSAEEQLDAGVKPGLLRLSVGIEHINDIIADLEQAFALIETAVAV"
     gene            2505..3536
                     /gene="metX"
                     /locus_tag="PLM90_12360"
     CDS             2505..3536
                     /gene="metX"
                     /locus_tag="PLM90_12360"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HMZ90220.1"
                     /translation="MQHNYCLPEGFQLENGQFIPELRLRYHTYGQLNAQRDNVIWVFH
                     ALTANAEPDIWWNGLFGEGNILDPSCYLIVCVNMPGSCYGSTGPEDTDRYGRKYAETF
                     PVFTIRDMVLAYQKLRDALQIKKILFAIGGSMGGMQALEWAVVEPDFIENLVLLACNH
                     QHSPWGIAYNAAQRMAIEADTSFLHGDGKKGLEAARAIAMLSYRNYRTFCLTQQHDET
                     VSQHKAESYLRYQGKKLSKRFSAQSYNILTKSMDSHNIARNRGDVHAALQRIKAKTLV
                     IGIDSDILFPVIEQAFLAKHIPGAALEVISSNYGHDGFLIESGKIKRAINSYFPSLNS
                     QRKLCKTIL"
     gene            3518..4699
                     /locus_tag="PLM90_12365"
     CDS             3518..4699
                     /locus_tag="PLM90_12365"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HMZ90221.1"
                     /translation="MQNNTLNIGLFGYGCVGKGFASLATNYPELNLRIKKICIRDAEK
                     PRDLDAEYFTTDANSLLLDDEINVIVELISDADAAFLIVKQALCNGKAVVSANKKMLA
                     EHLEELVSLQQQFGVPLLYEGSVCGSIPVIRNLENYYDKEPLLGIEGIINGSTNYILT
                     KLHANDIEYKTALAEAQALGFAEADPTLDVEGYDPVNKLCILLLHAFGIFIKPESIFH
                     KGITGINAVDIAFAKKHHSRIKLLAKATQTEAGIQAYVLPHLVNGNSNLIAIDFETNS
                     VAIHAAWSDLQQLTGKGAGSIPTGSAVLSDVLAIRKNYCYPYKKSVYNDNKPTLVDGI
                     PLRIYLRAEASSLVDTTIFEEIEETFKSEDNTFYIGKTKLENLSKASWVKDSGVSLIA
                     L"
     gene            4898..5470
                     /locus_tag="PLM90_12370"
     CDS             4898..5470
                     /locus_tag="PLM90_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HMZ90222.1"
                     /translation="MFSFQFSEIVTISLTLFAIIDVLGTVPAIINMKSRGIILQPGKT
                     GLFAGALMVTFLFLGDTILRLLGLDTQSFALAGSVVIFLLGLEMILGMHIFKSNTESG
                     SAGSLVPIAFPLLVGAGTLTVLISMRSIYSTVNILVAVVINSIVIYVVLRSTDWIEQR
                     VGKAGLHAITKFFGVIALALAIKIFLANMM"
     gene            5533..6141
                     /locus_tag="PLM90_12375"
     CDS             5533..6141
                     /locus_tag="PLM90_12375"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HMZ90223.1"
                     /translation="MKTALITIVFICTGLLIFACANNNGQSNMHPSAPEVRNATPNLP
                     LSSLMASAGLQAGDIWIYIDKSAFTLEIKHDTTTIKTYPVVLGGNPVGDKRMEGDQKT
                     PEGEFALRSIYPHKKWSKFLWVNYPTDESWEKFNAAKASGEIPKNASIGGEIGIHGVP
                     EGYDSLIDEMQNWTLGCISLKNAHIDEIYDAVKVGTRVVIKG"
BASE COUNT         1961 a         1327 c         1428 g         1915 t
ORIGIN      
        1 ctatcgacga taggagatgg agaggggccg ggggtgaggt gggcgggcgg gggtgaggcg
       61 gtttcgcgca aaggcgctaa gtggcaaagg cgctaaactc tcccactcac ccactgaccc
      121 attcacccac tcacacactg acccactcac cattcacgat tctaccttaa ctttactaaa
      181 aacatactat gaacgcagaa ttcttccttt gggtaaacgc agtgctttac gccggctttg
      241 ctgcctggtg tgctattagt ccaacgagca ccgcggcatt ttcgggattc agttttatga
      301 atgtaaatgg acgttcggaa tattttgcac tttatgtggg attgcaagct gcatggtcgg
      361 tgatgtttgc tatttgtgct attaataatg atctgcaata tgccggagta ttttatgccg
      421 tattcttata tgccggtgtc atcttcggca gatgggtttc tatatttcaa aaaggtgtgg
      481 ccggtaataa tgtgtacatc atcgccatat tagagatcat tcttggtgca tgggctgttt
      541 tgctgctcag caatttttga aaaataattt ggttcaagta aaacaattac aatattatca
      601 gataaatatt tggatgtctc atttctaaat atatatcttt gcatcagttc ttaagaatta
      661 cttatccaga aaggccgagg gactgacccg aagacgcctt agcaaccctc aaatgtttct
      721 cgcatttgtg aaggtgctaa tttcagaaag ataggttgga gagtcaaaaa tattgcaagc
      781 tcaccaatac atatcggcga gcttttttta tgcccgtcag tctcctgaaa tctttatgtg
      841 gtaagcaatt cacaagattg gaacacaatt tgctattaac acaaaaggta atgttcatca
      901 tgggtaaaaa cattaaaacc tacacattat tgtcgctgca aagcagggga attaattact
      961 caatttatca ttcattttcc ttcaggtata tgtgttgcca tatggcaact atgtaactat
     1021 tgcttccctt tccgggtgcg aatgatccga tacgttcggt agatcatgcg tgatatattc
     1081 atgcaccatt aatcggagaa aaatctccgc aagccactgc tatgcccaac attttcacaa
     1141 ttcaatttta aaatatcatt aaatcaaata tcatgagtaa ctatcaatat gaaacgttgc
     1201 aattacacgc cggacaacaa gcagatcctg taacaggaag tcgcgccgtg cctatttatc
     1261 aaactacatc ctatgtgttc aacgacagcg cacatgcagc agatctgttc ggattaaaag
     1321 cgttcggcaa tatctacaca aggattatga atccaacaac agatgtcttt gaaaaacgta
     1381 tcgctgcatt agaaggtggt gttgctgcac tggccgttgc atcgggacag gcagcccaaa
     1441 ccatcgccat taccaccatt gcacaggcgg gagataatat cgtttctaca tcttttttat
     1501 acggcggcac atacaatcaa tttaaagttg cattcaagcg attgggtatt gatgttcgtt
     1561 ttgccgaagg tgatgacatt gaggcatttg aggcactcat cgattcgaga acaaaaggta
     1621 tttacctgga aactataggc aatcccggtt ttaacattcc tgatctcgaa aaatttaccg
     1681 cacttgccaa acgccatgat attccactta ttgttgataa tacattcggt gctgcaggct
     1741 acatcgcgca gccgataaaa tatggcgcca atattgttat cgcgtcagca acaaaatgga
     1801 ttggtggaca cggaacaagt atcggcggtg taattgttga tgccggaaat ttcaattggg
     1861 gaaatggaaa gttcccactc ttcaccgaag catctgaagg atatcatgga cttaaattct
     1921 gggatacttt cggtgccggt tctcctttcg gaaatatcgc atttatcatt agagcacgcg
     1981 ttgaaggatt gcgcgatctt ggtcctgcca taagtccgtt taatgcattt ttacttttac
     2041 aaggtcttga gacattatca ctgcgcgttc agcgccattg cgacaatgca caacagcttg
     2101 ccgaatggtt agagcaacat cctttggtgg aatacgtaaa ttatcccgga ttaacatctt
     2161 caccctatca tgcgttagct gatcaatatc tgaccaatgg ctatggcgga gtcttgtctt
     2221 ttaaagtaca cggcggtaca acaaatgcaa acaaatttgt cgatgcctta aaattggtga
     2281 gtcacctggc aaatgttggt gatgctaaat cgctgattat tcatccggcc tcaactacac
     2341 atgaacaact aagtgcagaa gaacaacttg atgcaggagt aaaaccggga ttactacgtt
     2401 tatcagttgg cattgaacat atcaatgata tcatcgccga tttggaacaa gcatttgctt
     2461 tgattgaaac tgcagttgct gtttaatttc aaaaataatt catcatgcaa cataattatt
     2521 gccttcccga agggtttcaa ttagaaaacg ggcaattcat tcccgaattg cgattgcgtt
     2581 atcacactta tggtcaatta aatgcacagc gcgataatgt tatttgggta tttcacgcac
     2641 ttactgcaaa tgccgaacct gatatctggt ggaatggttt atttggtgaa ggcaatattc
     2701 ttgacccaag ctgctatttg attgtttgcg taaatatgcc gggttcttgc tatggtagca
     2761 caggtcctga agatacagat cgatatggaa ggaaatatgc tgaaaccttt ccggtattta
     2821 ccatacgcga tatggtgctg gcctatcaaa aattgcgaga tgcacttcaa ataaaaaaaa
     2881 tcttgtttgc tattggtggt tccatgggtg gcatgcaagc cttggaatgg gctgtagtgg
     2941 agcctgattt tattgaaaat ctggtgttgc ttgcatgtaa tcaccaacat tcaccttggg
     3001 gaattgcata caacgcagcg caacggatgg caatagaggc agatacttct tttcttcatg
     3061 gcgatggcaa gaaaggtctg gaagcagcgc gtgcaatagc tatgttatcg tatcgcaatt
     3121 atcgcacctt ctgtttgaca cagcagcatg atgaaactgt atcacaacac aaagcagaat
     3181 cgtacctacg atatcagggg aagaagcttt ccaaaagatt tagtgcccaa agctataata
     3241 tactgacaaa aagcatggac agccataata tcgcccgcaa tcgaggagat gtacatgcgg
     3301 cacttcagcg tattaaagca aagaccttgg tgattggtat tgattctgat atactattcc
     3361 cggtgattga acaagccttt ctggctaaac atattcccgg ggctgcattg gaggttatca
     3421 gcagtaatta tggtcacgac gggtttctga tcgaatcagg aaaaatcaaa agagccataa
     3481 attcatattt tccatcatta aactcacaaa gaaaattatg caaaacaata ctttaaatat
     3541 aggattattc ggatatggtt gtgtaggaaa gggatttgct tcactggcaa caaattatcc
     3601 tgaattgaat cttcgtataa aaaaaatatg tatcagagat gcagaaaagc caagagattt
     3661 ggatgcagaa tattttacga cagatgcaaa ctctttactt ctcgatgatg aaatcaatgt
     3721 catagtggaa ctcatcagcg atgccgacgc agctttccta attgtaaagc aagcattatg
     3781 caatggaaaa gctgtagtca gtgcaaataa aaaaatgctg gcggagcatc tggaagaact
     3841 tgtttcacta caacagcaat tcggtgtgcc gctgttatat gaaggttcag tttgcggaag
     3901 tattcctgtc attcgcaatc tggagaatta ttatgataaa gaaccactgc ttggcataga
     3961 aggtattata aatggctcca caaattacat ccttacaaaa ttacatgcca atgatatcga
     4021 atataaaacc gcattggcgg aggctcaggc attaggattt gcagaagccg accctacttt
     4081 agatgtagaa ggatacgacc ctgtaaataa actttgtatt ctacttttac atgctttcgg
     4141 aatttttata aaaccggaat caatttttca caaaggcatc acaggaatta atgcggttga
     4201 tatcgcattt gcgaaaaaac atcatagccg catcaaactc ctggccaaag caacacaaac
     4261 agaagccggc atacaagctt atgtgcttcc ccacctggta aatggcaact ccaacctcat
     4321 cgcaattgat ttcgaaacaa acagcgttgc cattcatgct gcctggtcag atttgcagca
     4381 gcttacaggc aaaggagccg gcagtattcc gacaggatcg gcagtattga gtgatgtatt
     4441 agccatccgg aaaaactatt gctatccata taaaaaatct gtgtataacg acaataagcc
     4501 tacacttgtc gatggtatac cactgcgcat ttatttgcgg gcagaagctt cgtcgctagt
     4561 tgacaccact atttttgagg aaattgaaga gacgtttaaa agtgaagata acacgtttta
     4621 cattggaaaa acaaaacttg aaaacctttc aaaagcctct tgggtaaagg attccggggt
     4681 tagcctgatt gcactttgac aaaaaaaaga aaggaagtga actttttaag gtcggtaatt
     4741 gtctgtatct ttgtaactgc aatgaaattt actttgaaca tatataacaa cagcatcggc
     4801 atccatgagt atccatggac atgcaagtgc catgcttgct aaaagcatac tcccctgttc
     4861 accgttgtta ttttttggca tttcaaagtt cacttttatg ttttcctttc aattttcaga
     4921 gatcgtaacg atcagcttga ccttattcgc aattattgat gtattgggaa ctgttcctgc
     4981 catcattaat atgaaaagtc gcggcattat actgcaaccc ggaaaaaccg gattgtttgc
     5041 aggagcattg atggtcacct ttctgttttt aggtgatacc attttgcgat tattgggttt
     5101 agacacacaa tcatttgccc ttgcaggttc tgtagtaatt tttctgctcg gactagagat
     5161 gatactcggc atgcatattt ttaaaagcaa taccgaatca ggcagtgccg gtagtctggt
     5221 gcctattgct tttccattgt tggttggcgc gggcacttta acagtgctca tcagcatgcg
     5281 cagtatatat tcaacagtga atattctggt agcggtagtt attaatagta ttgtcattta
     5341 tgtagtgctg cgctccaccg attggataga acaacgcgta ggtaaggcag gtttacatgc
     5401 catcacaaaa tttttcggtg tgattgcatt ggcattggcg attaagatat ttcttgcgaa
     5461 tatgatgtaa aacctattat agattacaaa gaatcgcaaa tacgaatatt gatacgtcat
     5521 cgttaatatt ccatgaaaac cgcactgatc acaattgtct ttatttgcac gggattactg
     5581 atatttgcct gtgccaataa taatggtcaa tctaatatgc atccttctgc accagaagta
     5641 agaaacgcca ctccgaattt accattgtca tcactgatgg caagtgccgg tttacaggca
     5701 ggagatatat ggatatatat cgataaatcg gcattcacgc ttgagataaa acatgatacc
     5761 accaccatta aaacatatcc tgttgtatta ggcggaaatc ctgttggcga taaacgcatg
     5821 gagggcgatc aaaaaacgcc tgaaggcgaa tttgcgttac gttcgatata tccgcataag
     5881 aaatggagca aatttctatg ggtgaattat cctacagatg agagttggga aaaattcaac
     5941 gcagcaaaag catcaggtga aattccaaaa aacgcaagca ttggaggaga aattggtatt
     6001 catggcgtcc ccgaaggcta cgactcgcta atcgatgaaa tgcaaaattg gacactcggt
     6061 tgtatctcct taaagaacgc tcatattgat gaaatttatg atgcggtgaa ggttggtact
     6121 cgggtagtta ttaaggggtg attgggtgat tgggtgagtg ggtgagtggg tgagtggtga
     6181 gtggtgagtg gtgagtggtg agtggtgaat ggtgaatgta attcggaacg ttttgtgaag
     6241 agtgattcct ccgcaaactc cgcaaactcc gctaactccg cgattgaccg tgtgcgattg
     6301 gttgacctgt gaattgtgag cttgcctcgc taccggcggt gaatttatag agccaagtat
     6361 ctagactttg cgccttagcc tccttgctgt ctttgcgcga aaccaacccg cctcacctcc
     6421 ggcccctctc catctcctat cgtcgataga gaggggagtt gattgcgctt cggaattaaa
     6481 atattgcaaa gcaatataat tgcattccct attaacgcgc cagcgttaaa agcactccac
     6541 attggctaat cagctaattg gctaattaac cacacttgct aaccctcttt cagtatctag
     6601 aatcaagtat ctagtatcta gacttttgcg c
//
LOCUS       DAOQOU010000175         6607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6653_length_6607_cov_6.180250, whole genome shotgun sequence.
ACCESSION   DAOQOU010000175 DAOQOU010000000
VERSION     DAOQOU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6653_length_6607_cov_6.180250"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1250
                     /locus_tag="PLM90_12380"
     CDS             279..1250
                     /locus_tag="PLM90_12380"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ90224.1"
                     /translation="MDLEVAFEVIYLKLLIPQLAMHTRQSTYIQRINDALRYIDLHID
                     AELSLDVVAKVAFYSPFHFHRLFKAVTGEALLVYITRRRIEHSGLMLMHRPSMSVSEI
                     AYAFGFSSVAVYSKTFKKVMGLSPTAFRDELNIKNSKIGQLDSKIGQDDLISEAYICN
                     INNLKKWLMMQAKISIKDTHDLHFAYLTQIGVDGIESTFQQVLKWARPKAILSKDETF
                     ISRVFHDSFKVTDESKVRMSIGIASRHELPDDQHMSSGVISGGKTFIGRFEITLEEFE
                     PAWNSIFIEMHDRGYNKADGFPYELYHNNPANHPEKKYIVDLCIPIE"
     gene            complement(1257..3059)
                     /locus_tag="PLM90_12385"
     CDS             complement(1257..3059)
                     /locus_tag="PLM90_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ90225.1"
                     /translation="MKKLVIICCFLSCYLTAGAQTDAALAQQYMSTGQYDKAADLYKN
                     LFNDNPNGYYKAYYNCLLVLQDYKEVEKIIDKQIKKNPDNLTYYVDYGNVLEKQGDKK
                     NATKQYESAIQKMTDSRPQVTQLANAFIGYDMPENAIRVYERARDIVSTYSFNYELAG
                     LYYRAGSFDQALDTYLDYYVEDPMGGSAKVTSAFSRILDEEKDHTLLQEKLFARIQNG
                     KYELQYTELLIWDYIQQKDFDGALIQAKALDKRNKENGERVYDLGETARAEESYDAAI
                     TAFEYIISKGKGSPYYFSAKNGILNCHKDKIFKLGQYTKSDIDALRNSYTAFLAEYDR
                     KDARAASVTDDLAKLEAFYAADFAKAISLAEPLVDWPAISAADRSRIKLDLGDFYLIS
                     GDVWEATLLYAQVDKAMKDEPLGEEARFKSAKLAYYRGDFSFAQGMLDVLKAATSELI
                     SNDAMQLSVFITDNLGLDSIAEPMMLYAKADLLLFQNNIQGALKVLDTLTTTYPAHQL
                     SDDVLFLKAKIAIKQQDYTTAVNYLEQIRQNYAYELLGDDAIYMLGDIYQYQFKDNEK
                     AQLCYEQIFMNFKDSLYSTEARKRYRQLRGDKIN"
     gene            complement(3129..3902)
                     /locus_tag="PLM90_12390"
     CDS             complement(3129..3902)
                     /locus_tag="PLM90_12390"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMZ90226.1"
                     /translation="MEQSSPFLTDTFDEIVFHGRNKRYGSFVLRSGIRKNTFIGLYLM
                     TALTLLYTGIHLIDFSIDRSLPPSNFSNVEVTLSEPPPLFEVLPPAVPPAAAPQNLEK
                     VDEMEVKPDNEVSDIKPLTTDDKQIDSTSTGTATSGSTNNSAVTGDGNTIYSNVEVYP
                     QYPGGPKGLKKYLSDNVQYPEIAHKNNITGTVWVVFVVNQDGSVSDVKVEKGIGGGCD
                     QEAIRVVQGMRRWKPGMDGGKPVRVYQRIKIGFALDDNS"
     gene            complement(4340..4720)
                     /gene="gcvH"
                     /locus_tag="PLM90_12395"
     CDS             complement(4340..4720)
                     /gene="gcvH"
                     /locus_tag="PLM90_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMZ90227.1"
                     /translation="MQFPAHLKYTKDHEWILIEGTTGTIGITEFAQSELGDIVYVELN
                     GLGDHMNQNDVFGTVEAVKTVSELFMPVSGEITEINAGLDSAPESVNKDPYGDGWMIK
                     VTMSDLSQVDGLMSAAEYQAHIGH"
     gene            complement(4837..>6607)
                     /gene="sprA"
                     /locus_tag="PLM90_12400"
     CDS             complement(4837..>6607)
                     /gene="sprA"
                     /locus_tag="PLM90_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMZ90228.1"
                     /translation="INYRHTVSATYTLPISKLPIFSWITIKANYNTSYGWVAGSIGIA
                     DTLGNTINNTQSKSVNGEFNFRNLYNKSKFLKQYNSNTSPKAEKKSGKGDEEATDEKA
                     ADENTKTAKKPSTPTVSPPVETLVRLLISLKRMTVNYSQNYATTLPGFMPSSDILGLN
                     AATTAPGWDFIFGYQPSYKWMDSIARKGWISSATSLNYLFLQTYSENLDVRANFEPLK
                     DFRLDLNLNKTYSKNHSEYFKNTNSIDDPLFSHLNGVDAGSFNISYSIMGTVFEKLDA
                     KEVSATFKQFEANREIISARWAALNTIYADDIFFNPLDGTFLPNYTEGYGPYSQDVLI
                     PAFLAAYTGKDANEVSLNPFDQMPKPNYRITYNGLSKLPGFKRLFSSFNVTSAYTSTL
                     ALNSFITDLDFDGNYFAYGHVVDTLTGNYVTLYDIPNIIVNEALSPLIGFDVTWINGV
                     TSKFDFKKSRTLTMSFIDYQLTQINSTEFTIGAGYKMSGFTFPFKIRGKRPNLENDLT
                     FKFDFSFRDDITTNFRLDQDLNEPTGGMKSISLSPTIDYVVNDRFNVQLYFDRQQTIP
                     KISTSYPITSTNAGVKVRFSLAE"
BASE COUNT         1920 a         1290 c         1372 g         2025 t
ORIGIN      
        1 tcgacgatag gagatggaga gggcgggggg tgaggcgggg taattagcaa atttgagaat
       61 tagcaaatta gctaattggg ggtgtgctag catgaggact attgtcatag tgaggaagtt
      121 tgagggttag cgagttagca ggtgaggtgg tttcgcgcaa agactgccaa ggggcaaagg
      181 cgcaaagtct gctacacccg cacatgaaca cccgaacaca taaacacaac aatgttaaaa
      241 tccaatttag ctatttgaca gtagcttaaa ataattgctt ggatttggaa gtagcatttg
      301 aggtgatata tttgaagtta ttaataccac agttggcaat gcatacccga caatctacat
      361 atatacaaag gatcaatgat gcacttagat atatcgattt acatatcgat gctgagttgt
      421 cgttggatgt tgttgcgaag gtggcgttct attcgccatt ccacttccac aggctgttta
      481 aggctgttac gggtgaggca ttgctggtgt atattaccag aagacgcata gaacattcag
      541 gccttatgtt aatgcaccga ccttcaatga gtgtttcaga aattgcctat gcttttggat
      601 tttccagtgt tgcagtttat tcaaaaacct ttaaaaaagt aatgggtttg agtccaactg
      661 catttcgtga tgaacttaat attaaaaata gcaagattgg acaactcgac agcaagatcg
      721 ggcaagatga cctgataagt gaggcataca tttgcaacat taataatctt aaaaaatggt
      781 taatgatgca agcaaaaatt tcaattaagg acacacatga tcttcatttt gcctacctca
      841 ctcaaattgg agttgatggc attgaatcta catttcaaca agtactgaaa tgggcccggc
      901 ctaaagctat tctttcaaag gatgaaacgt ttattagcag agtctttcat gatagcttta
      961 aagtaacaga tgaaagcaag gttcgcatga gcattggaat tgcctcccgc catgaactgc
     1021 cggacgatca acatatgagc agtggtgtta tatccggtgg gaaaacattt attggtcgct
     1081 tcgaaataac acttgaggaa tttgaacctg catggaatag tattttcata gaaatgcatg
     1141 ataggggata taacaaagca gatggatttc cttatgaatt atatcacaac aatccggcaa
     1201 atcatcctga aaagaaatac attgtggatc tatgtattcc aattgagtaa tttcgatcaa
     1261 tttattttat caccacgaag ctgacgatat cttttgcggg cttcggtgct atacagcgag
     1321 tccttgaagt tcatgaagat ctgttcgtaa caaagttgag ctttctcgtt atccttgaac
     1381 tgatattgat aaatatctcc gagcatataa atggcatcat ctcctaataa ttcgtaggca
     1441 taattctgac gaatttgttc aagataattt actgcagttg tataatcttg ttgtttgatg
     1501 gcgatttttg cttttaagaa tagaacatca tcactaagtt gatgagcggg gtaagtggtt
     1561 gtaagggtat cgagcacttt caacgctccc tggatattat tttgaaataa aagtaaatct
     1621 gcctttgcat acagcatcat cggttcagca atagaatcca gtcccaaatt atcggttatg
     1681 aaaacagaga gctgcatggc gtcatttgaa atgagctcac tggtagcagc tttgagcaca
     1741 tccagcatac cttgagcgaa gctgaaatct cctcgataat aggcaagttt tgcactttta
     1801 aatcgtgctt cttctcccag cggttcgtct ttcatggctt tatcaacttg agcataaaga
     1861 agagtagcct cccaaacatc gccgctgatg agataaaaat cgccgagatc aagtttaatg
     1921 cgtgaccgat ccgcagcaga aattgcgggc cagtcaacca gtggctccgc caatgaaata
     1981 gcctttgcga aatcagctgc ataaaatgct tcaagctttg ctaagtcgtc ggtaacagat
     2041 gcggcgcgtg catcttttct gtcatattcg gctagaaaag cggtatagct gtttcgcaac
     2101 gcgtcaatat cggatttcgt gtattggcca agtttgaaaa ttttatcctt atggcaattt
     2161 aaaattccat tttttgcgct gaaataatat ggtgaacctt ttccttttga aataatatat
     2221 tcaaatgctg taatggcagc atcgtaagat tcttcggcgc gcgcagtttc tccaagatcg
     2281 taaacgcgtt cgccattttc tttattgcgt ttatccagtg ccttagcctg tatcagtgcg
     2341 ccatcaaaat ctttttgttg aatataatcc caaatgagta attcggtgta ttgcaattcg
     2401 tattttccat tttgaatacg tgcaaacaat ttctcctgta aaagtgtatg gtctttctct
     2461 tcatcgagta tacgcgaaaa tgccgaagtc acttttgcac ttccacccat aggatcttcc
     2521 acatagtaat cgagataagt gtctagtgct tgatcgaaac tccctgcccg ataatacaaa
     2581 ccggcaagtt cgtagttaaa ggaataggtg ctcacgatat ccctcgctcg ttcataaaca
     2641 cgaatcgcat tttccggcat gtcatatcca ataaatgcat tggcaagttg tgttacctgc
     2701 ggtcgactat ccgtcatttt ttgaatggca gattcatact gttttgtagc atttttctta
     2761 tcaccttgtt tttccaatac attgccatag tcgacataat aggtcagatt atccggattt
     2821 tttttgatct gtttgtcgat aatcttttct acttccttat aatcctgtag cactaacaaa
     2881 cagttataat aagctttgta atatccattt ggattgtcat tgaacagatt tttatagaga
     2941 tcagctgctt tatcatattg accggtactc atatattgct gagccagcgc ggcatccgtt
     3001 tgcgctcctg ctgtcagata gcaggataaa aagcaacata taatcactag ttttttcata
     3061 caataagcgt atctaaaata atgcccggat tactgttttt agtattccga caacacaaat
     3121 taacggaatc agctattatc atccagagca aatccaattt ttattcgttg atataccctt
     3181 acaggtttcc cgccatccat acctggtttc cacctcctca ttccttgtac aacacgaatt
     3241 gcttcctgat cgcagcctcc gcctattcct ttttccactt tcacatcaga aacactgcca
     3301 tcctgattca ccacaaatac tacccaaact gtgccggtaa tattattttt atgagcgatt
     3361 tcaggatatt gaacattatc ggatagatat ttttttaatc cttttggtcc tcccggatat
     3421 tggggataaa cttctacatt cgagtaaata gtatttccat cacctgtgac agccgaattg
     3481 tttgtgctgc cggaagtggc ggtgcctgtt gaagtgctat caatttgctt atcatcggtg
     3541 gtaagaggct tgatatcgct tacttcattg tcgggtttta cttccatttc atctaccttc
     3601 tcaagattct gtggagcagc agcgggtgga acggcaggtg gaagcacttc gaaaagcggc
     3661 ggtggttcac ttagggtaac ttcaacattg ctaaaatttg aaggaggtaa gcttcgatct
     3721 atggaaaaat cgattaaatg aatgcctgta tacaaaagtg taagagctgt catcaaatac
     3781 aacccgataa aggtattttt tcgaatgcct gagcggagca caaatgagcc atagcgtttg
     3841 ttgcgaccgt gaaaaacgat ctcatcaaac gtgtcggtta aaaatggtga tgattgctcc
     3901 attgacattc aagtatcctc aatacaaaaa acattccgaa agtaacgctg ttcagcgagc
     3961 ttgccgtttt ttgctaactg aaattaacaa taccaagccg gcgccaagca agcagccgag
     4021 gctatttgct aagatatcat aaacgtcaaa ttgccttgtt ggcagcgctc tttgtattaa
     4081 ttcgatgcta attccatata aaatacataa acctatagcc ctaaaaatgc tctttttgag
     4141 ggtatagata actgttaagc ggattaaagt ctgagcggtg aggaacgtaa gtatgccata
     4201 aaccaggaaa tgcatcacct tatccggttc ccaaattgac aaagagggta gctcgtcgct
     4261 cggaattacc gatacaacaa ggataactaa agcccaaaga atgcttaaaa cccggaagat
     4321 ttttcctgaa aatgacatat tagtgtccga tatgcgcctg gtattcagca gcgctcatca
     4381 gtccatctac ctgtgaaaga tcagacattg tgaccttgat catccaacca tcgccataag
     4441 gatctttgtt taccgattcg ggagcgctat ccagtccggc attgatctca gtgatctcac
     4501 cgctaaccgg catgaacaat tcagatacgg ttttcacagc ttcaacggtg ccgaaaacat
     4561 cattctgatt catatgatct cccaacccat tcagttctac atatacgata tctccaagtt
     4621 cactttgggc gaattcggtg ataccgatag tgcctgtagt gccttcgata aggatccatt
     4681 catggtcttt ggtgtatttg aggtgggcag gaaattgcat aactgttctt ttgggcaaaa
     4741 ataggggttt tggccgaatt gttttagtga tttttagtac gtgggaaatc gttcagcatt
     4801 tcagatgctt acatgaaatt cttactccat tcgcccttat tcagcaagac tgaagcgaac
     4861 cttaacaccc gcattggtgc tggtaatagg atacgaagtt gagatcttag gaattgtttg
     4921 ttggcgatcg aaatacaatt gcacattaaa gcgatcgttc acaacatagt caatggttgg
     4981 cgacaatgaa attgatttca taccacctgt cggttcattc aaatcctgat cgagacggaa
     5041 atttgtcgtg atatcatcgc ggaaactgaa gtcgaattta aatgtgaggt cgttttccag
     5101 atttggtcgc ttgcctctaa ttttgaatgg gaaggtaaat ccactcattt tatagccggc
     5161 accaatagtg aactccgtag aattaatttg tgtgagctga tagtcgataa agctcatggt
     5221 gagtgttctc gattttttga aatcgaattt agaagtaaca ccattgatcc atgtaacatc
     5281 aaaaccgatg agtggtgata aagcctcgtt cacaatgata ttaggaatat catataatgt
     5341 aacatagtta cctgttaagg tatccactac gtgaccatat gcaaaataat taccatcgaa
     5401 atcaagatca gtgataaagc tgttcaaagc aagcgtagat gtatatgcac tggtcacgtt
     5461 aaatgatgag aacagtcgtt taaatccggg caatttgctc aatccgttat acgtaatgcg
     5521 ataattcggt tttggcatct gatcgaaagg attcaatgaa acctcgttag cgtctttacc
     5581 ggtatatgcc gcaaggaaag caggaatcag cacatcttga gaataaggac catatccttc
     5641 ggtataattc ggaaggaagg tgccatcaag tgggttaaag aatatatcgt cggcataaat
     5701 ggtattcaaa gcagcccatc tagcagaaat gatctcacga tttgcttcaa attgtttgaa
     5761 tgtagcagat acttctttcg cgtcgagttt ttcaaatact gtacccatga tagaataact
     5821 gatattgaaa cttcctgcat caacgccatt caagtgactg aataacggat cgtcgatgga
     5881 atttgtgttt ttgaaatatt cgctgtgatt tttagaataa gttttattca aatttaagtc
     5941 gagacggaaa tccttcagcg gttcaaaatt ggcacgcacg tcgaggttct cgctataggt
     6001 ttgaaggaac aggtaattga gtgaggtcgc tgaactaatc catcctttgc gcgcaatgct
     6061 gtccatccat ttataggaag gctgataacc gaaaatgaaa tcccaaccgg gtgctgtggt
     6121 tgccgcattt aatccgagta tatcagatga aggcatgaat cccggaagtg ttgttgcgta
     6181 attctgtgaa tagttcacag tcattctttt caagctgata agcaatctta ctaaggtttc
     6241 taccggagga gaaactgttg gggttgaagg ttttttagca gttttggtgt tttcatctgc
     6301 tgccttttca tcagtagctt cttcatctcc cttaccactt ttcttttcag cttttggcga
     6361 ggtgtttgaa ttgtactgtt tcaggaattt cgatttatta tacaggttgc ggaaattaaa
     6421 ttcgccatta acggatttag attgtgtatt gttgatggta tttccaagtg tatctgcaat
     6481 gcctattgaa cctgcgaccc aaccatagct ggtattgtaa ttcgctttaa tcgtaatcca
     6541 actgaaaatt ggcaatttgc tgataggtaa agtataggta gccgaaacgg tatggcgata
     6601 attgata
//
LOCUS       DAOQOU010000176         6570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6708_length_6570_cov_5.164543, whole genome shotgun sequence.
ACCESSION   DAOQOU010000176 DAOQOU010000000
VERSION     DAOQOU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6708_length_6570_cov_5.164543"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..873
                     /locus_tag="PLM90_12405"
     CDS             145..873
                     /locus_tag="PLM90_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90229.1"
                     /translation="MQKLIWISIFICTILSCKESNITAQMHQGEHLVIVDSVDAESRF
                     VDYDFYKRHGVYSIYYLGADTDTIQLGKHKINGFDDGYEDFQNRNDFTLPDPSKLSIY
                     VDTLIPLKQIIRVSGKGIRYYELKDTLVINAFPIIVRNHCDSLIRLGIFSEICYMVRQ
                     AQDETGDWVDIENTIRFGCSTGARDIVIKPDSLIIALLPRYEGDYKTACRLRFMHGKH
                     AVYSNVFYDMIDKRQFSEPLPDQY"
     gene            899..1498
                     /locus_tag="PLM90_12410"
     CDS             899..1498
                     /locus_tag="PLM90_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90230.1"
                     /translation="MNSSILMLCVILLSACTSNHVSEPRFQFSQIDIVSYKWTSDTIQ
                     HDWQLYIASIGSIDSLGQCAIARRDTFKGTERYFKQVLPMAFDSLIAKINYKELPTSF
                     FIKDDVVRIYDGFTYCLLWHQQDTIVSTVFEPDKIPHGALRDFIMQLNDFIQQADTES
                     STSFDYSEINRYILEHVVDSSRLPPYRYVIEFKRTDSPK"
     gene            complement(1559..1708)
                     /locus_tag="PLM90_12415"
     CDS             complement(1559..1708)
                     /locus_tag="PLM90_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90231.1"
                     /translation="MGKDLVKERREERGERRKERGERRKEKGERKKEKGERKEEISVR
                     RWKKY"
     gene            complement(1690..2796)
                     /locus_tag="PLM90_12420"
     CDS             complement(1690..2796)
                     /locus_tag="PLM90_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90232.1"
                     /translation="MKKVNLGIAQNKFDQIQQRSENVLHLSIAKLVGHIENPTTYPMT
                     GDNKSIERALFNHYNILAKSEQKDFINRIKKFSNIPSNTTQFQSRFGDLAKVDLKSHT
                     SVAEQIKSIAVPDSLKFTASEIDELEKKLLKKRVGAKSTPVTKQPAVVAPRLIRLTVE
                     SIHCTNTVELKKDEILMNALFTDALGVSTTIDLVDVGKMKDGETINLGAKGIIAEVNL
                     NNAGFFPQTLIGTFTLIDKDVFADQEKIVSAFRTCAIIATTLTSIALISMGVAIAVAA
                     TPGGMAAAAGIMTGALIAMVGSLVILVVGGGITLALQGETSDLIKDEFVFDLNPVTLQ
                     PGEFVTRVLNVEIPRSIGKKSGNYTMTIKWERIS"
     gene            2995..4392
                     /locus_tag="PLM90_12425"
     CDS             2995..4392
                     /locus_tag="PLM90_12425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ90233.1"
                     /translation="MTCAIVGGLTLPLLIQDGMFMDALLYTSVANNLSTGYGTFWFPQ
                     FSLHNVSDFNAFHEQPPLGIFIQSLFFRAFGHSMYVERFYTFLMLIITARLMVSLWKR
                     VYLSESILHQYSWLPVLLWMLSPICFWSYSNNMLENTMGVFTLSAVILLFDAMRSQKT
                     SKFILGGLFIFLATMIKGVPGLFPLATPCIIWIMERNSGFVKMIWQSFLSLITVALLY
                     CCMLLIPDARESYDVYFLQRTLFRIDSLPTVNSRFYILGRLFLELLPQFILLIIVLLI
                     ARNKKRIPGAQYFKQGLIFILIGISASLPLMLTMVQKAFYFVPALPLFAIGLASMMAP
                     VIAIENRKENLRPSTLKVWRGVGVLLFIGMLGYTGAMFGHVKRDKDLIHDVRLIGDAI
                     PNEHVIGTPNAMWNNWSMQTYMMRYNNMSMDEGYKQTYVVVDQKYQTQIPEFYTKIDI
                     PTKRYHLFKRKESGI"
     gene            4398..6023
                     /locus_tag="PLM90_12430"
     CDS             4398..6023
                     /locus_tag="PLM90_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ90234.1"
                     /translation="MQTRNKIFYFLAVCSLFVVACTHHDTEDKKIFHLNLASPVTSLD
                     PAFSSDQPNTWSVNLLFNGLVQLDSDLQVQPCIASHWELSPDRLRYTFHLRNDVYFHN
                     DACFPGGKGRRVVAEDVVYSFHRLIDPKVAARGNWVFNNIVDTLLPFTAINDSTIEIR
                     LLKPFAPFIQRLSIPYCSVVPHEAIEKYGLDFRSHPVGTGPFVFKQWDEGELIILHRF
                     DNYFERDNAGNRLPYLDAVNIHFIANKSTEFLKFLSGDLDFVSDIDAAMKEDILTKEG
                     NLQDKYAEKINLLKGPYLNVEYFSILMDTSNVLVKSSPLSMEEVRKAINYGFDRKEML
                     LFLKNNRGVAATCGIVPPELLPAGPVGYGYDYHPDSALVLLKRAGFENGVGLPEIVIQ
                     TIEQYQDIAVYLKDKLEDIGIAIRIETIDPRLMREMRLNGTTVFFRSSWIADYADAEN
                     YFTLFYGLSDAPPNYTRYKNATFDSLYTIAVAEPDAVVRGKLYRSMDSLIMEDAPVVP
                     LFYDEIYRFTQKNITGLEPNGLNMLDLRRVKKD"
     gene            complement(6028..6432)
                     /locus_tag="PLM90_12435"
     CDS             complement(6028..6432)
                     /locus_tag="PLM90_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ90235.1"
                     /translation="MFNVRVYGLWMHDGNILTVEEPVRGQMIRKFPGGGLEFGEGIIE
                     CLRREFREELSMEISDIHHFYTTDFFVQSMIDPSHQVISIYYTVSGDPALAIFDKEEQ
                     LQFKWMPINDLHPDLFALPIDKKVVSLLCEHQ"
BASE COUNT         1823 a         1336 c         1282 g         2129 t
ORIGIN      
        1 ggtttgaatc ctgtcgaggt cacttttctt ccttcgtttt cttggagtat atttggtagg
       61 gtagcgctgt ctagtatcta gtctggtaac agcttggcgc ctttgccact ttgcgtctta
      121 gcgcgagcaa aacacatcca tcgcatgcaa aaacttatct ggataagtat tttcatctgc
      181 accattctta gttgtaagga gagcaatatt actgcgcaaa tgcaccaagg cgagcacctt
      241 gtaattgtgg attctgttga tgcagaaagc aggtttgtag attatgattt ttataaacga
      301 catggcgtat attcaattta ctacctgggt gcagatactg atactattca actcggcaaa
      361 cacaaaataa acggctttga cgacggctat gaagattttc aaaaccgcaa tgattttacc
      421 ttgcccgatc cctcgaaatt gagtatttat gtcgacaccc ttattccttt gaaacaaatc
      481 atcagggttt caggaaaagg tattcgttac tatgaattaa aagataccct tgttattaat
      541 gcctttccta tcatcgtcag aaatcattgc gattcattga ttagactagg gatattcagc
      601 gagatctgct acatggtccg tcaggcacaa gatgaaaccg gggattgggt ggatatcgaa
      661 aatacaattc gcttcggatg cagcaccgga gcgcgcgaca tagtcattaa acctgatagc
      721 ctgattatcg cactgctgcc aagatacgaa ggcgattata aaactgcttg ccgacttcga
      781 tttatgcacg gcaaacatgc tgtgtattcc aatgtgtttt acgatatgat tgacaaacga
      841 caatttagcg aaccactgcc ggatcaatat tgatattatg catgactata aaatgaaaat
      901 gaactcgtca atcttaatgc tatgtgtcat tttattaagt gcatgcacat caaaccatgt
      961 ttccgagccc aggtttcaat tttcccaaat agatattgta tcatacaaat ggacctcaga
     1021 taccattcaa catgattggc aactctatat agcatcaatc ggcagtatcg acagcttggg
     1081 tcaatgtgcc atcgcgcgta gagatacatt taaaggtaca gaacgatatt tcaaacaggt
     1141 acttcccatg gcatttgatt cactgatcgc taaaataaat tataaagaat tacctacatc
     1201 attttttata aaagacgatg ttgtgcgcat ctatgatgga tttacttact gcctgctatg
     1261 gcatcagcaa gataccattg tatctactgt tttcgaaccg gataaaattc cacatggcgc
     1321 cttacgcgat tttatcatgc aattgaatga ttttattcaa caggctgata ctgaaagttc
     1381 aacgtcattc gactattctg aaatcaatcg gtatatacta gagcatgttg tggattcatc
     1441 aagattgcca ccgtatcggt acgtgattga atttaagcgc acagactcgc cgaaataagg
     1501 tagcaaaacg gttgccaacc gccctaaata tcgcaatgct tgcagaccac tgtatctact
     1561 aatatttctt ccatctcctt acacttatct cctctttcct ttctcctttc tcctttttcc
     1621 tttctccttt ctcctttctc ctttctcctc tctcctttct cctctctcct ctctcctctc
     1681 tcctctcttt cacgagatcc tttcccactt gattgtcatt gtataattcc ctgatttttt
     1741 accgattgac cgtggaatct ccacattaag gactctggta acaaattcac ccggctgtaa
     1801 tgttacaggg tttaagtcaa atacaaattc atctttaatc aaatccgaag tttcgccctg
     1861 aagtgccagg gttattccac cgcctacaac taatattacc aaagagccaa ccatggcaat
     1921 taatgcacct gtcataatgc ctgctgccgc agccatacct cccggagttg ctgctacagc
     1981 tattgcaaca cccattgata ttaatgcaat ggatgtcagt gtagtggcaa taatggcaca
     2041 tgtcctgaat gcactgacaa ttttttcctg atcagcaaaa acatctttat caatcaacgt
     2101 aaatgttccg attaaggtct gagggaaaaa tcctgcatta tttagattga cttcagcaat
     2161 aataccttta gcccctaaat ttattgtttc gccatccttc attttgccta catcaacaag
     2221 gtcgatagtg gttgatacac ctaatgcgtc ggtaaacagg gcattcatga gtatttcgtc
     2281 tttctttaat tcaacagtat ttgtgcaatg tatgctttca actgtcagtc taattaatcg
     2341 tggcgcaaca actgcaggtt gttttgtgac gggtgtggat tttgctccaa cgcgcttttt
     2401 taataacttc ttttcgagct catcgatttc tgaagcggta aactttaaac tgtcgggaac
     2461 tgcaatactt tttatttgtt ctgctactga agtatggctt ttgagatcca ccttcgccaa
     2521 atcaccaaag cgcgattgaa actgagtagt atttgatgga atattgctaa atttttttat
     2581 gcgattgatg aaatcctttt gttcggattt tgctaatatg ttatagtgat tgaataatgc
     2641 acgctctatc gacttgttat cgccggtcat tggatatgtt gtcggatttt caatgtgccc
     2701 taccaatttg gcaatcgata aatgtaagac gttttcacta cgctgttgga tttgatcaaa
     2761 tttgttttga gcaatgccta aattgacttt tttcatgtta atggttttgt gttttgagat
     2821 tgttattagt tctttgagtc tgtattaaag gtaataattt gtataactta aatccatatt
     2881 tatttcccgt tttcttattc atttcagcct taatagtgga ctaaacctcg tattttagca
     2941 cttgcgatgc cattatcgga gaattcctca tccttccaca gatctatgtg gcttgtgacc
     3001 tgtgccattg tgggcggact tacactgcca ctgcttattc aggatgggat gtttatggat
     3061 gcactgctat acacctccgt agctaataac cttagcaccg gttacggaac attttggttc
     3121 ccgcagttca gcctgcataa tgtatctgat ttcaatgcat ttcatgagca acctcctctg
     3181 gggattttca ttcaatcatt attcttcaga gcatttggac atagcatgta tgtagaacgg
     3241 ttttatactt ttctcatgct gattattacc gcacgattaa tggtatcctt atggaagcgc
     3301 gtttatcttt ctgaaagcat attacaccaa tattcctggc tgcctgtttt attatggatg
     3361 ctgtcgccaa tttgcttttg gtcttacagc aataatatgc tcgagaatac catgggtgtt
     3421 tttacgctgt cggcagtgat tttgttgttt gatgccatgc gcagtcagaa aacatcaaaa
     3481 tttattttgg gtggattgtt tatttttctt gccactatga tcaaaggagt gcccggttta
     3541 tttccgctgg caacaccatg cataatctgg ataatggaaa gaaattccgg ctttgttaag
     3601 atgatttggc aatcattttt atcactcata acagtggctt tgctttactg ctgtatgctc
     3661 cttattccgg atgcacgtga aagctatgat gtgtattttt tacagcgtac tttattccgc
     3721 atagattcac ttccaaccgt aaatagcagg ttttatatat tgggcagatt atttcttgaa
     3781 ctattgcctc agtttatttt gcttatcatt gtgttgctga tcgcacgcaa taaaaaacgc
     3841 attcctggtg cgcagtattt taaacaagga ttgatattta ttctaatcgg tatttcagca
     3901 tctcttcccc tcatgctcac catggtgcaa aaagcatttt attttgttcc ggcacttccc
     3961 ttgtttgcta taggattggc ttcaatgatg gcaccggtta tcgctattga aaatcgcaag
     4021 gaaaatttac gtccctcaac attgaaagta tggcgtggtg ttggtgtcct tttgtttatc
     4081 ggcatgctcg gctataccgg tgctatgttc ggtcatgtta agcgcgataa agatcttata
     4141 catgatgtta gattaattgg agatgcaatt ccgaatgagc atgttattgg cacaccgaat
     4201 gccatgtgga ataactggag catgcaaact tatatgatgc gttataacaa catgagtatg
     4261 gatgagggtt ataaacaaac ttatgtggtt gtagatcaga aatatcaaac gcaaattccg
     4321 gaattttata ccaaaataga tatcccaact aagcgatatc atctgttcaa aagaaaggaa
     4381 tcaggcatct agtgatcatg caaactcgta ataagatatt ttacttcctt gctgtatgca
     4441 gcttatttgt tgtggcatgt acacatcacg atacggagga taaaaaaata ttccacctca
     4501 atcttgccag tcctgttaca tcgcttgacc ctgcgttttc cagcgatcaa ccgaatacct
     4561 ggagcgtaaa tttattgttc aacggacttg tgcaactgga tagtgatctt caagtgcaac
     4621 catgtattgc atctcattgg gaattatcgc ctgaccgctt gcgatatacc tttcacttgc
     4681 gcaacgatgt ttattttcat aatgatgcat gtttccccgg tggaaagggt aggcgagtgg
     4741 ttgcagaaga tgtggtgtat tcatttcacc gacttataga tcctaaagtg gcagcacgtg
     4801 gaaattgggt atttaataat attgtcgaca cgctattgcc atttactgca ataaatgatt
     4861 caacaataga aattaggctg cttaaacctt ttgcgccatt tattcagcga ttgagtattc
     4921 cgtattgttc tgtcgttcca catgaagcca ttgaaaaata tggacttgat ttcagaagtc
     4981 accctgtcgg aacggggcct tttgtattta aacaatggga tgaaggtgaa ttgatcatct
     5041 tacatcgatt cgataactat tttgaacgcg ataatgccgg aaaccgtctt ccatacctcg
     5101 atgccgtaaa tattcatttc atcgcaaata agtcgaccga atttttgaaa ttcctcagtg
     5161 gcgatctgga ttttgtgagt gatattgatg cggctatgaa agaagatatc ctcacgaagg
     5221 aaggcaattt gcaggataaa tatgccgaga aaataaattt attgaaaggt ccttatctca
     5281 atgttgaata tttctccatt ttaatggata cctcgaatgt attggtgaaa tcaagtccgc
     5341 tttccatgga ggaagtgcgt aaagccatta attatggttt cgatagaaag gaaatgttat
     5401 tgttcctcaa aaacaacaga ggtgttgccg caacttgtgg catagtgcct cccgaattat
     5461 tgcctgccgg accggtagga tatggatatg actaccatcc ggatagtgca ctagtgctgc
     5521 ttaaaagagc ggggtttgaa aatggcgtag gcctaccgga aattgtaata cagactatag
     5581 agcagtatca ggatatcgca gtttacttaa aagataaact tgaagatatc ggcatcgcca
     5641 ttcgcattga aaccatcgat ccgagattga tgcgtgaaat gcgattaaat ggcacaacag
     5701 tatttttcag atcaagctgg attgcagatt atgcagatgc tgaaaattat ttcactttgt
     5761 tttatgggtt aagcgatgca cctcctaatt atacaaggta taaaaatgcc actttcgata
     5821 gcttgtatac tattgcggtt gcagagcccg atgcggtggt gcgcggaaaa ttataccgca
     5881 gcatggacag cctcataatg gaggatgcgc cggtggtgcc attgttttat gatgaaattt
     5941 atcgcttcac gcaaaaaaat atcacaggtc tcgaacccaa cggattaaat atgcttgact
     6001 tgcgaagagt gaaaaaagat tgagcaatta ttgatgttca cacaaaagtg acactacctt
     6061 tttatctatt ggtaacgcaa ataaatcagg atgcaaatca tttataggca tccatttaaa
     6121 ttgcagctgt tcttctttgt caaatatggc aagagcagga tctcctgaaa cggtataata
     6181 aatacttatt acctgatgcg aaggatctat catactttgg acaaaaaaat cagtggtata
     6241 aaaatgatgt atatcgctga tttccatcga caactcctca cgaaattctc gtcgcaggca
     6301 ttcaatgatc ccttcaccaa attcgagtcc gccacccgga aattttctga tcatttgacc
     6361 acgcaccggt tcttccacgg ttagaatatt tccatcgtgc atccagagac catagacgcg
     6421 aacattaaac atgttgggca ttcaattgct cgagcgttga gcgtattgct tcgaaatttg
     6481 gcagctctcc tgcattttca agtatttctg cataccgtac tatgccttct ttgtcgatga
     6541 tgaatgcgct tcgtttggaa acacctttca
//
LOCUS       DAOQOU010000177         6430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_6932_length_6430_cov_7.242980, whole genome shotgun sequence.
ACCESSION   DAOQOU010000177 DAOQOU010000000
VERSION     DAOQOU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6932_length_6430_cov_7.242980"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLM90_12440"
     CDS             complement(<1..651)
                     /locus_tag="PLM90_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90236.1"
                     /translation="MRTTIPVFIFILLAGSMSAQITKSAFFVGNSYTATNNLPQLISD
                     VAESAGDVLLYNSNAPGGYTFEMHTTNTTTLEQIALGTWDYVVLQEQSQLPSFPIDQV
                     EVDCFPFATTLDSLIRVANTCTETMFFMTWGRKDGDASNCAFWPPVCTYNGMDSLLHE
                     RYMTMGEDNDAEVSPVGAVWHYLRDTHPEIELYIGDGSHPSAAGSYAAACTFYAAIF"
     gene            complement(744..4040)
                     /locus_tag="PLM90_12445"
     CDS             complement(744..4040)
                     /locus_tag="PLM90_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ90237.1"
                     /translation="MFTSTKKKLLTLSACVSVFAYQHADAQIDAGLFRFPDVSATQIV
                     FTYANDVWIAPIEGGTAIKLSSPSGVESFPKFSPDGKTIAYTANYDGNWDVFSIPTTG
                     GLPARLTQHGWTDRVVDWTPDGKSVLFASGRESEKERYSKLFTIPAQGGAPTTLPLAY
                     GEFASYNADGSSMALVFRSEAFRTWKRYRGGDVADIYIFNFADNSSYNISGDIDAGEE
                     FPMWYGNAIYFLSDNGSEKRMNIWKYDMATKSRTQITHFTDYDVHFPSMGPNDIILEA
                     GGKMYLYGLQSGNLREVKLNVVTDNTNLKPHTENVAALMQNMQIGPDGNRVVVEARGE
                     LFSIPAENGYVKNLTRNSGTAERYPAWSPNGKWIAYWSDASGEYELWTMDMENNGATK
                     QITHLGKGYRYYIYWSPDSKKVVFVDQAMKIWMVDMATGVATNVDHGLQQAHYNLNGF
                     TANWSPDSRYIAYLRDLDNAYYAAFIYDTQDKTIHQVTSGFYNCSEVTFDPSGKYLYV
                     ATGQSFSPTYSDLDNSFIYPNTTELAAISLKKDTPSLLVAKNDMVEIKKEEAKEEKAD
                     DKKDKNKKDKSEKEEDSASTIAPTEIDWDGIEGRMVLLPIPAGNMYNVSGVEGKIIFI
                     RYPNTGTAEGSTPSLKYYDIEKREEKTILDGVWDYALTADGKKVLVMGDGMQAIVAAE
                     EGQKFEKPLRINEMEMVVDPMAEWKQIFMDTWRIERDYFYDVNMHGVNWEQVKERYLK
                     MLAGATTREEVNVVLGDMIAELNSSHAYRFGGDLEATPMANCGYLGINWTADGQYYRV
                     GKIIRGASWDAEMRSPLDAPGIGIVEGDYILAVNGNPILTTQEPFAYFQGLGGKTVEL
                     TYNNKPSMDGAKTVVVTTLDYEGRLRNLAWIEQNRKRVEEASNGQVGYIYVPSTGVDG
                     QNELIRQFRAQWDKPALIIDERFNNGGQIPDRFIELLDRDPLAYWAIRDGKSWQWPPL
                     AHFGPKVMMINGWSGSGGDAFPDFFRQKNLGPLVGQRTWGGLIGISGAPTLIDGGEIT
                     APTFRMYYQNGTWFAEGHGVDPDIKVEEDLTSLAKGKDAQLERAIEEAMKLIKTEGYQ
                     APARPAPEVR"
     gene            complement(4168..4938)
                     /locus_tag="PLM90_12450"
     CDS             complement(4168..4938)
                     /locus_tag="PLM90_12450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ90238.1"
                     /translation="MEYLRIIGGTGLSLIFLAIVFIPMEKVFPAKPGQKILRPYWKTD
                     LCYFLGQYLLWGFLVLWAMMQLSFFLDDIVPHSFRTAVQLQPKWLQIIEVVLGSDLLI
                     YWGHRLQHRIDFLWRFHKIHHSAEHLDWLAAHREHPLDSLYTIGLINLPAFLLGFHLD
                     LLAGLIAFRGIWAIYIHSNVRLRLGPLRVLIGSPELHHWHHDLDRDRGNYANISPLMD
                     VIFGTHVFPDHEPEHFGIKEEFPKSYLAQLLAPFRRTK"
     gene            complement(4959..5324)
                     /locus_tag="PLM90_12455"
     CDS             complement(4959..5324)
                     /locus_tag="PLM90_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90239.1"
                     /translation="MKTQTLLRAGTLVFFFCMIGGFVAYKSGLIQLSENNIPQAQQPD
                     YINTLASNTKGVIDTPPGLQTTLPEIPDSVFRIMMSSKTMIILDQSSLFPDNDNQFPE
                     HFYDDSIFELPRITLDSLK"
     gene            complement(5346..5771)
                     /locus_tag="PLM90_12460"
     CDS             complement(5346..5771)
                     /locus_tag="PLM90_12460"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HMZ90240.1"
                     /translation="MRLTLAMICAFMVSMSAKAQNWQDNILGGWINGDKDAKVEIYVK
                     DGKYYGKITWLRNPNEEDGTPKVDDENPDESKQNEPIMGLVILKGFDFVDGMWKNGTI
                     YDPKNGKTYKCEMWLDGKELKIRGYWGIVYRTETWTKPK"
     gene            complement(5882..>6430)
                     /gene="nadD"
                     /locus_tag="PLM90_12465"
     CDS             complement(5882..>6430)
                     /gene="nadD"
                     /locus_tag="PLM90_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HMZ90241.1"
                     /translation="SFNPVHTGHMIIANYFAENTDLQQVWFVVSPQNPFKDKQSLLDE
                     KHRLYMVNLAVEQHYKLQASNIEFHLPQPSYTINTLTYLKEKHPDYTFVLLMGSDNLS
                     SLHKWKNIDLILASHDIYVYARRGVETIPDGLGGNIRLFQLPMLDLSSTTIRQFIKEG
                     KSIRYLVPDVVWNHINEMRFYR"
BASE COUNT         1784 a         1481 c         1204 g         1961 t
ORIGIN      
        1 aaaaatggct gcgtaaaatg tacatgctgc ggcgtaagat cctgctgccg aagggtgact
       61 gccatcgcca atatataatt cgatttcagg atgtgtatcg cgcaaataat gccagacagc
      121 acctacagga gagacctctg catcattatc ttctcccatt gtcatatagc gttcgtgcag
      181 caaactatcc atgccattgt acgtacacac cggaggccag aatgcacaat tcgatgcatc
      241 gccatccttt cttccccaag tcataaaaaa catggtttcg gtgcatgtgt tcgccaccct
      301 gattaaacta tccaacgttg ttgcaaaagg aaaacaatct acttccacct ggtctattgg
      361 aaaggatggg agctgacttt gttcttgtaa caccacataa tcccatgtgc ctaatgcaat
      421 ttgttcgagc gtggtggtat tggtggtatg catttcgaat gtatatccac cgggggcatt
      481 actattatat agtaatacat cacctgctga ttcagcaaca tctgaaatta attgtggcaa
      541 attattcgtt gcagtataac tattgccgac aaaaaatgca ctttttgtaa tctgtgcaga
      601 catcgaccct gctaacaaaa taaaaatgaa gaccggaatt gttgttcgca tgtactaaaa
      661 ttctaaatga agatacgaca caaataaaaa agcgactgac agttgaatca attcaattgt
      721 cagtcgctcg taatatttgc agattatctt acttcgggag caggacgcgc aggcgcctga
      781 tatccttcag tctttatcaa tttcattgct tcttcaatgg cacgctccaa ttgagcatct
      841 ttaccttttg caagtgatgt aagatcttct tctactttaa tatccggatc aacaccatgg
      901 ccttctgcaa accatgtacc attctgataa tacattctga acgttggcgc cgtaatctcg
      961 ccaccatcta tcaatgttgg tgcaccgctg ataccaatca aaccacccca ggtgcgttgt
     1021 ccaaccagtg gtcccaaatt tttctgtctg aagaaatcag ggaatgcatc accccctgag
     1081 ccactccaac cattgatcat cattactttg ggtccaaaat gcgctaacgg aggccattgc
     1141 caagattttc catcgcgaat agcccaatat gccagcggat cgcgatctaa taattcaata
     1201 aagcgatcgg gaatttgtcc gccgttatta aatcgttcat caataatcaa tgccggttta
     1261 tcccattggg ccctgaattg acgaatcaat tcattttgtc cgtcgacacc ggtactaggc
     1321 acataaatat atcctacctg accattagat gcttcttcca cgcgctttct attttgttct
     1381 atccaggcaa gattgcgcag acgcccttcg taatccaatg ttgttacaac aaccgttttt
     1441 gcgccatcca tacttggctt attgttgtat gtaagttcta ctgtctttcc accaaggcct
     1501 tggaaatatg caaatggttc ttgtgtggtg agaatgggat ttccatttac cgcaagaata
     1561 taatctcctt caacaatacc tattccaggt gcatcaagcg gcgaacgcat ttctgcatcc
     1621 cacgatgctc cacgaattat ttttccaacg cgataatatt gtccatccgc cgtccagttt
     1681 attccaaggt atccgcaatt tgccattggt gttgcttcca ggtcgccacc gaaacgatat
     1741 gcatgcgagc tgtttaattc ggcaatcata tcgcccaata caacattcac ctcttcgcgc
     1801 gtagttgcac cggctaacat cttgagataa cgctctttta cttgttccca attaacgccg
     1861 tgcatattca catcataaaa ataatcgcgt tctatgcgcc aggtatccat gaatatttgc
     1921 ttccattcgg ccatgggatc tactaccatt tccatttcat taatgcgcaa tggtttttca
     1981 aatttctgac cctcttcagc tgctactatc gcttgcatgc catcgcccat caccaatact
     2041 ttttttccat cagcagtaag cgcataatcc catacaccat cgagaatagt tttctcttcg
     2101 cgcttttcaa tatcataata ttttaaactt ggtgtgcttc cttcagcagt acctgtattg
     2161 ggatatcgaa taaatattat ttttccttca actccggata cattatacat atttcctgca
     2221 ggaataggca atagtaccat tctgccttct ataccatccc aatcaatttc ggtaggcgca
     2281 attgttgaag cggagtcttc ttctttttca gatttatctt ttttgttttt gtctttctta
     2341 tcatcagcct tttcttcttt cgcttcttct ttctttatct ccaccatatc gtttttcgcc
     2401 accaataagg atggcgtatc ttttttcaaa ctaatggcag ctagttcggt tgtgttagga
     2461 taaataaacg aattgtcgag atcgctgtat gtcggtgaaa acgattgccc cgttgcaacg
     2521 tataagtatt ttccggaagg atcaaatgtt acttccgagc aattataaaa tccactcgtc
     2581 acctgatgaa tggttttatc ttgcgtgtcg tatataaatg ccgcataata tgcattatcc
     2641 agatcgcgca agtaagcaat atacctgcta tcgggcgacc agtttgcggt aaatccattt
     2701 aaattataat gcgcctgctg aagaccatga tctacatttg ttgcaacacc ggtagccata
     2761 tcaaccatcc agattttcat ggcctgatca acaaatacaa ccttcttgct atcaggcgac
     2821 cagtatatgt aatatcgata gccttttccc aaatgtgtaa tttgttttgt tgcgccattg
     2881 ttttccatat ccatggtcca caattcatat tctccggatg catcgctcca ataggctatc
     2941 cattttccat ttggcgacca agcaggatag cgctctgcag ttccgctatt gcgagtgagg
     3001 ttttttacat atccgttttc tgcgggaata gaaaataatt caccgcgcgc ttcaaccact
     3061 accctattac catcgggacc aatttgcata ttctgcatca gcgcggcaac attttcggta
     3121 tgtggtttca ggtttgtatt atccgtaact acattaagtt ttacttcacg caaattaccc
     3181 gattgcaatc catacaaata catttttcca cctgcttcca aaatgatatc attaggaccc
     3241 attgatggaa aatgcacatc ataatcggtg aaatgcgtga tctgcgtacg cgattttgta
     3301 gccatgtcgt acttccagat attcatgcgt ttttcagagc cattatctga tagaaaataa
     3361 atggcattgc cataccacat tggaaattct tcacctgcat caatatcgcc tgaaatatta
     3421 taggaactat tatcagcaaa attaaatatg tagatatcgg caacatcacc gccgcgataa
     3481 cgtttccagg tgcggaacgc ctctgaacga aaaaccagtg ccatgcttga accatcggca
     3541 ttataactgg caaactcacc ataagctaat ggcaaagtgg taggcgcacc accttgtgcc
     3601 ggtatggtga ataatttcga ataacgctcc ttttcactct ccctcccgga cgcaaacaat
     3661 accgattttc catcgggtgt ccagtcaaca accctgtcgg tccagccgtg ttgggttagt
     3721 cgcgccggca gcccacccgt agttggtatg ctgaagacat cccagttgcc atcgtaattt
     3781 gctgtataag caattgtttt tccatcagga gaaaattttg ggaagctttc aactcctgaa
     3841 ggagaactta atttaatagc cgttccacct tcaataggtg cgatccagac atcattggca
     3901 taggtaaata caatttgtgt ggcggacaca tcaggaaatc tgaatagtcc ggcatcaatt
     3961 tgtgcatcgg catgctgata tgcaaacacg gatacgcaag ctgacagggt gaggagtttt
     4021 ttctttgtgg atgtgaacat aaaaaagatg ttatgcggct aaattacgct ttgccaacaa
     4081 ttaatagggt atttgatgac aggccttaac aataattatt gaaggtaata aatacaatag
     4141 attttagctt gcgacagatt gtaaatacta tttagttctt ctaaatggtg ccaggagctg
     4201 tgccagatat gactttggaa attcctcttt gatgccaaaa tgttcaggct cgtgatccgg
     4261 aaatacgtgt gtgccaaaga tgacatccat caatggagaa atattggcat aattccccct
     4321 gtcgcgatcc agatcatgat gccagtgatg caactccggc gatcctatca aaacacgtaa
     4381 aggcccgagt cgaagtcgca catttgaatg aatatagatc gcccatattc ccctgaatgc
     4441 aattaatccg gctagcaggt ctaaatgaaa tccgagcaaa aaagcaggaa gattaatcaa
     4501 tccaatggta tacaaactat ctaatggatg ttctctatgc gccgccaacc aatcgagatg
     4561 ttctgcactg tgatgaattt tgtgaaatcg ccacaaaaaa tctatgcggt gttgcagtcg
     4621 atgtccccaa taaatcaata ggtcactacc taaaaccacc tcaataattt gcaaccattt
     4681 tggttgtaat tgtacggcag tacgaaatga atgcggaaca atatcatcca ggaaaaaact
     4741 caattgcatc attgcccaca ataccaaaaa tccccataat aaatattgcc ctaaaaaata
     4801 acacaaatcc gttttccaat aaggtctgag aattttttgt cccggttttg cagggaatac
     4861 tttttccatg ggaatgaaaa caatggcaag aaaaataagg cttaatcccg taccgccaat
     4921 tattctgaga tattccatgg ataggcgtaa ttactgactc actttaaact atctagtgtt
     4981 atgcgcggta attcaaagat gctatcgtca taaaaatgtt ccggaaattg attgtcgtta
     5041 tcaggaaaca atgatgactg atcaagaatg atcattgttt ttgacgacat cataattcta
     5101 aaaacgctgt caggaatctc cggtagtgtg gtttgcagcc cgggtggtgt atcaattacg
     5161 cctttggtat tgcttgctag tgtattgata taatcaggtt gttgtgcctg aggaatatta
     5221 ttttctgaaa gttgaattaa tccggattta tacgcaacaa accctcctat catacagaaa
     5281 aagaaaacga gtgtgcctgc gcgcagcaat gtttgtgttt tcatgttagc gcgtttattt
     5341 tatctttatt taggcttggt ccatgtttct gtgcgatata caatgcccca atatccacgg
     5401 atctttaatt ctttaccatc tagccacatt tcacatttat atgtttttcc gtttttggga
     5461 tcatagatcg ttccgttctt ccacatacca tctacaaaat cgaaaccttt taaaattacc
     5521 aatcccatga tgggttcgtt ctgcttcgat tcatccggat tttcatcatc aacctttggt
     5581 gtgccatctt cttcattcgg attgcgtagc caggtgatct tgccgtagta ttttccgtct
     5641 ttcacataaa tctcaacctt ggcatcctta tcgccattta tccagccacc taatatatta
     5701 tcttgccagt tttgtgcctt agcgctcatg cttaccataa acgcacaaat cattgccaaa
     5761 gtcagtcgca tatcattctt gtttgttccc taaaaatacg attctcccgg aaatttcttt
     5821 ccggttatat aaaacgcctg ctgacatatt attcttatac tgcgataagg cttaccgccc
     5881 atcaacgata aaagcgcatc tcattaatat gattccacac gacatcggga acgagataac
     5941 gtattgattt tccttctttt ataaactgtc gtattgtggt agagctgagg tccagcattg
     6001 gaagctgaaa taatctgata tttcctccca acccatccgg aatggtttca acccccctcc
     6061 tggcataaac atagatatca tgcgatgcga gtatcagatc gatattcttc cacttgtgca
     6121 aactcgacag attgtcgctg cccatcagca gcacaaaggt gtaatcaggg tgcttttctt
     6181 tcaggtaggt aagcgtgttg atggtataac tgggctgtgg caggtgaaac tcaatgttgg
     6241 aggcctgcag cttgtaatgc tgctccaccg ccaggttcac catatataac ctgtgttttt
     6301 catccagcag tgattgcttg tctttgaagg ggttttgcgg actcaccaca aaccatacct
     6361 gctgcaggtc ggtgttttcg gcaaaataat tggcaatgat catgtgacct gtgtgcacag
     6421 gattaaaaga
//
LOCUS       DAOQOU010000178         6394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_7000_length_6394_cov_5.927749, whole genome shotgun sequence.
ACCESSION   DAOQOU010000178 DAOQOU010000000
VERSION     DAOQOU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_6394_cov_5.927749"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLM90_12470"
     CDS             complement(<1..433)
                     /locus_tag="PLM90_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90242.1"
                     /translation="MQIRVTAQRKYDLRFLFRRYVLIVVDVLLILTALFLYIRFFHPS
                     ALQSYHTFYDNLLWVVMIVTLWFIYSYIFNLYKLSNVDRARLTIRNTVLTAALTGLTY
                     LFIPYLSPTLPYSRTPAFVLIGAMVVLVLLWRLCYAVFLKHP"
     gene            complement(449..1408)
                     /locus_tag="PLM90_12475"
     CDS             complement(449..1408)
                     /locus_tag="PLM90_12475"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HMZ90243.1"
                     /translation="MELAILKNRLNATIAISAIYFLVWSVVVILFPNLLSSVVIGQGN
                     TPIVFWDFMSIITGVLGISLFIAAFNPHKHWLIILMASLFHLAMLAGFIVGYQHGIFS
                     SLYLPFLFFNHIIWLIPNSIVLFSVYRRSLDTDDMLLDAFNSKDYPLELFDTTDGRNI
                     GDMAAESPLLIVFLRHFGCPFCKESLLQLADYRKELEARGMTIVLVYMVNEHIAKEYL
                     SSYGLDDLPQVSDPEEIFYKRFQLRRGSFSQLFGLKVWSRWIELGFKKKLFNTKPEGD
                     VAQMPGIFLLNQGRVVQQFIHQSVADTPDYSVFLSHNAIRTND"
     gene            1481..2332
                     /locus_tag="PLM90_12480"
     CDS             1481..2332
                     /locus_tag="PLM90_12480"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HMZ90244.1"
                     /translation="MKKILTIGLLLITAATQKLSAQELELPQPSPWAQVSQKFGLAQA
                     TVTYSRPSMKGRTIFGSLVPYGEMWRTGANKATELKLEGNVAINGQTLPAGAYSLFTI
                     PGEKEWTVIINKNTELWGTGGYKQEEDAMRFQVKVQSHTSTESFTIGFSNIKDNSAVL
                     EIYWETTMISFELTNDFLEQGKTNITEAIAAAENTMSLYNDAAEFYLDNNLDAKQALE
                     WAKKSVAQRERYWNLFTLAKAYAKNGMNKEAIETAEKALTLSNEAKNTGFSQGIEKEL
                     NKWKSNK"
     gene            2415..3131
                     /locus_tag="PLM90_12485"
     CDS             2415..3131
                     /locus_tag="PLM90_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HMZ90245.1"
                     /translation="MTTKPVFLTAKWQNLLMANYVVDPEILDPWLPYGTAHDTFDGHC
                     FLSLVAFEFNNTRVKKIAFPWHKNFVEINLRMYVKRIENNQLKRGVVFIKEIVPRHLI
                     SFFANTLYNENYVTMPTGGGLFRTNEHDFVGYTWGKKYSMEAKLAPEKKLLIPGDISE
                     FITEHYWGYASINNNSTYEYAVEHPRWETRDIIKHTIQVDFATLYGDTFSFLNVATPH
                     SVLLACGSDILVRHPNKIKP"
     gene            3146..>6394
                     /locus_tag="PLM90_12490"
     CDS             3146..>6394
                     /locus_tag="PLM90_12490"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ90246.1"
                     /translation="MYNLRYLTLSLLIIPMFLYGQLDQPKPALTADAPQWVQLMYTET
                     PNAYAIQDAFDTYYKTHEFEENNYTRYFQRWAILARPYVLENGDVHFPTHMQLMREIN
                     SRTRSQVIDEGSRSTTWTFAGPDIHFTTKYDAGATQIPVSDHMNTYSFDRSKSNPNIA
                     YCGGETGGIYKTTDQAMTWTHMTHGYYINTVRALAVDPFNPDIVIASSEGELWRTTDG
                     GITWNIMGDAAFQAIYITAYDIVFNPENSNIVYAGCEQGFFRSTDGGSNWTEILDHFC
                     MSVEVKPDNAAIIYTLQYDPTTQIPYFKKSFDYGATFTTYSDGWFTVPAADMGKIESL
                     GGRIGVTEANPDKVYVLLVGESDADAGLQLRGTIGVYMSNDSGESWSFPHGLIGMPYN
                     VDTHPNLMDFDGHSSDYDQIYYNTTIAVSQLDENKLLIGGLNLWKSEDGAVSYDPVGG
                     YLGYLPYYHPDNQETKIYKTSPTTEEIWMATDGGVNYTTDWVASHESRSNGLRGSNFW
                     GFDQGWQEDMMVGGRYHNGNGAYHENYPEGEFLALGGGEAASGYVKQSDENICMFSDI
                     GGIKLPDEIEGIAEHLAFSNAPNESYWYNSSSRIQFDWDYFDHAYMGRDNIIWKSTDG
                     GGGFTPLHEFGTNANNKVLWVEQSRVNTNIIYCTQNIGSTNCYLWKSTDRGLTWTNLT
                     LPQAKRDLIFTLSGDNADELWIAYKYGPNGSKVYHSINGGDSWVNITTATLNDMHPSA
                     IVHQYGTDGGVYVFMREAKVYYRTNTFIDWLQHGNDLPLVIDPLKAIPFYRDDKIRLG
                     SWNIGIWEAPLYEPSTLIADFSAEKPNFYCAGDQIKYVDHSTAGAGATYAWEFPGGIP
                     ATSSEQNPVVTYPTEGVFDATLTITDGAEVQSITKSAIVSSLAPSTLPFSENFETAQF
                     PEGWHGGTSGGWGIAEDASAYDVGTYSMFFNNYWTDLQGDHVDEITAQYDIDAAITAQ
                     LTFDVAYRPYGGIYMDTLAVYVSNDCGATLDLIYYKGGNTLATGPAYTADVFYPAADE
                     WRTETIDLTPYIDDVSLMLVFRNIGYWGQAMYADNINIAVEKEGITSHEENIS"
BASE COUNT         2064 a         1226 c         1397 g         1707 t
ORIGIN      
        1 tcggatgctt cagaaataca gcatagcaca atcgccataa taaaaccaaa accaccatgg
       61 caccgattaa tacaaatgca ggggttcttg aatatggcag cgtaggactt aaataaggaa
      121 tgaataaata agtcaatccg gttagcgcag ctgtgagcac agtatttctg atggtcagcc
      181 tggcgcgatc gacattactt aatttataga gattgaaaat gtaggaataa ataaaccaaa
      241 gggtaacgat cattactacc cagaggagat tgtcataaaa cgtatgatac gactggagtg
      301 ctgaaggatg gaaaaaccga atatacagaa ataatgcggt aagaatcagc aatacatcca
      361 caacgatcag gacatacctt ctaaacagaa agcggaggtc atattttctt tgtgcagtca
      421 cgcgaatctg cacaaaaata agcatttgtc aatcgttggt gcgaatggcg ttatgcgaca
      481 agaagactga ataatcaggg gtatcggcta cggattgatg aataaattgc tgcaccaccc
      541 gaccttgatt gagcagaaaa attccgggca tttgggctac atctccttct ggtttggtat
      601 taaagagctt ctttttgaag ccaagctcta tccaccgcga ccacactttc aacccaaaca
      661 actgcgaaaa gctgcctctg cgcaactgaa agcgcttata aaatatctct tccggatcgc
      721 ttacctgagg cagatcatca aggccatagc tgctcaaata ttcctttgcg atatgctcat
      781 tcaccatata aacgagcaca attgtcatac cccgggcttc aagctccttg cggtaatcag
      841 ccagctggag gaggctttct ttacagaagg gacacccaaa atggcgaaga aaaacaatta
      901 aaagtggtga ttccgccgcc atatcgccaa tatttcgtcc atctgtggtg tcaaacaact
      961 caagcggata atccttgctg ttaaacgcat ccaggagcat atcgtcggta tcgaggcttc
     1021 tgcggtaaac actgaagagc actatggaat taggaatcag ccaaatgata tgattaaaaa
     1081 acaaaaaagg aaggtaaagg ctgctgaaga ttccgtgttg gtatccgaca atgaatccgg
     1141 ccaacattgc cagatggaaa aggcttgcca ttagaatgat caaccagtgt ttatgggggt
     1201 tgaaggctgc aataaacagg ctaataccta agacgccggt gataatactc atgaaatccc
     1261 aaaaaacaat tggggtatta ccttgcccta taaccacgct ggagagcagg tttgggaata
     1321 gaataaccac tacactccac accagaaaat agatcgcact gatggcaata gtggcattga
     1381 gcctattttt gagaatagct agttccatat ttggatatca gggagtaaac aagcaggggt
     1441 ttgatttgtt tacctttgca tcaacaaaaa tagatactca atgaaaaaaa ttcttactat
     1501 cggtctgtta cttatcacgg cagccacaca gaagctttcc gctcaggagt tagagcttcc
     1561 tcaacctagt ccctgggctc aggtaagcca aaaatttggt ctggcacagg ctacggtaac
     1621 ctattcacgc ccatctatga agggtcgcac catttttggc tctcttgttc cttacggcga
     1681 aatgtggaga acaggtgcca ataaggctac tgagttgaaa ttggaaggaa atgtagccat
     1741 caatggtcag acattacctg ccggtgcata ttcacttttt acaattcccg gagagaaaga
     1801 gtggaccgta atcatcaata aaaacacaga attatgggga acaggcggct ataaacaaga
     1861 agaagatgca atgcgttttc aggtgaaagt gcagtcgcac acatcaacag aatcgtttac
     1921 aataggtttt tcgaatatta aggataacag cgccgttctt gaaatttact gggaaacaac
     1981 catgatcagt tttgaactga ctaacgattt cctggaacaa ggcaaaacca atattactga
     2041 ggcaatcgct gcagcagaaa atacgatgag cttgtataat gatgcagctg aattttatct
     2101 cgataataac ctggacgcca aacaagcact tgagtgggct aaaaaatcag tagctcagcg
     2161 cgaacgctac tggaacttgt ttacactggc aaaagcttat gctaaaaacg gcatgaataa
     2221 agaagctatt gagaccgctg aaaaggccct aacattgtca aatgaagcca aaaacaccgg
     2281 tttctctcaa ggtattgaga aggaactgaa taaatggaaa tcaaataaat aatgatattc
     2341 tactgacaca aagccatcct tcggggtggc ttttttttgc ccatacagtc caatcacatt
     2401 ggtatctttg tataatgacc acaaagcctg tttttctcac cgccaaatgg caaaatctac
     2461 tcatggcaaa ttatgtggta gacccggaga ttcttgaccc ttggcttcca tatggaactg
     2521 cacatgatac ttttgatggt cactgctttt taagtttggt ggcgtttgaa tttaataata
     2581 cgcgcgtcaa aaaaattgct ttcccctggc ataaaaattt tgtggagata aatcttcgca
     2641 tgtatgtgaa aagaatagaa aataatcaat tgaagcgagg tgtcgtattc attaaagaaa
     2701 ttgttccgcg acatttaatt tcattttttg caaatacctt atacaacgaa aattatgtaa
     2761 caatgccaac aggcggtgga ttgtttcgca ccaatgagca tgattttgtc ggttatactt
     2821 ggggcaagaa atatagcatg gaagctaagc tggcacctga aaagaaatta ttgatacccg
     2881 gagacatatc cgaatttatt acagagcatt attgggggta tgcttccatc aataataact
     2941 ccacctatga atatgctgtt gaacatccac gatgggaaac cagagatatc atcaagcata
     3001 ccatacaagt agattttgcc actttatatg gtgatacatt ttcatttctg aatgttgcaa
     3061 caccgcactc agtattatta gcatgcggca gtgatatatt agtacgccat ccaaataaga
     3121 tcaaaccata attaaaatat ttttaatgta taacctgcga tatctgacac tctcgttgct
     3181 gataattcca atgttcctat acggtcagct tgatcagcca aaacctgcat taacagctga
     3241 tgcgccgcaa tgggtgcaac tcatgtatac tgaaacaccg aatgcttatg caattcaaga
     3301 tgcattcgac acctattata aaacgcatga atttgaggaa aataattaca cccgatactt
     3361 tcaacgctgg gcgatattag caagaccata tgtattggaa aatggcgatg tgcatttccc
     3421 aacgcacatg caattgatgc gcgaaataaa tagcagaaca cgctcgcaag tcattgatga
     3481 aggaagcaga agcacaacat ggacattcgc aggacccgac atacatttta ctacaaaata
     3541 tgatgccggc gcaactcaaa ttccggtgtc agatcacatg aatacctata gttttgatcg
     3601 cagcaaaagt aatccaaata ttgcctattg cggaggtgaa accggtggta tatataaaac
     3661 cacagatcag gcaatgacct ggacgcatat gacgcatgga tattatatca atacggtaag
     3721 agcactggca gtagatccat ttaatcctga tattgtaatc gcttcatcag aaggtgaatt
     3781 atggcgtaca acagatggtg gtattacctg gaatattatg ggtgatgctg ctttccaggc
     3841 gatttacatc accgcttatg atattgtgtt taatccggaa aacagcaata ttgtttatgc
     3901 aggatgtgag caaggatttt tcagaagtac tgatggcggc agtaactgga cagaaatatt
     3961 agatcatttt tgtatgtcgg tggaagtaaa acctgataat gcagcaataa tttacacatt
     4021 acaatacgac ccgacaacac aaattcccta tttcaaaaaa tcatttgatt acggtgcaac
     4081 attcaccact tacagcgatg gctggtttac ggttcctgct gccgatatgg gaaaaattga
     4141 gagccttgga ggcagaatag gtgttaccga ggccaatccc gataaagtat atgtattact
     4201 ggttggtgaa agcgatgcag acgcaggatt acaattacgc ggcaccatag gtgtttatat
     4261 gagtaatgac agtggtgaaa gttggagttt tccgcacggt ttaattggaa tgccgtataa
     4321 tgttgacaca catccaaacc tcatggattt cgatggacat tctagcgatt atgatcagat
     4381 ttattacaac acaacaattg ctgtttcaca actggatgaa aataaattgc tcataggtgg
     4441 attgaatttg tggaagagcg aagatggagc agtctcctat gatccggttg gtggatattt
     4501 aggatatctg ccttattatc atccggataa tcaggagaca aaaatttata aaacatcacc
     4561 tacaacagaa gagatatgga tggcaacaga tggtggtgta aattatacta ccgactgggt
     4621 tgcatcgcat gaaagcagaa gtaatggctt gcgcggaagt aatttctggg gatttgatca
     4681 gggatggcag gaagatatga tggtgggagg aagatatcac aatgggaacg gcgcctatca
     4741 cgaaaattat cctgaaggcg aattcttggc actcggtggt ggcgaggctg catccggata
     4801 tgtaaagcaa agtgatgaga atatctgtat gttcagcgat attggtggta tcaaattacc
     4861 ggatgagata gaaggtattg cagaacacct cgcattttcg aatgcaccaa atgaaagcta
     4921 ttggtataat tcaagttcac gcatacaatt tgactgggat tattttgatc acgcatatat
     4981 gggtcgtgat aatattatat ggaaaagcac agacggtggc ggtggattta ccccgctgca
     5041 tgaatttggc actaatgcga ataataaagt gttgtgggtt gaacaaagca gagtaaatac
     5101 aaacattatt tattgcacac aaaatatcgg ttcaacaaat tgttacctct ggaaaagtac
     5161 ggatagagga ttaacatgga ccaacttaac acttccgcaa gctaaacgcg acctcatatt
     5221 tacattaagc ggtgataatg ccgatgaatt atggattgcc tataaatacg gtcctaacgg
     5281 aagcaaggta taccactcta taaatggtgg tgattcatgg gttaatatca caacggctac
     5341 gttaaatgat atgcatcctt cggcaatagt gcatcagtat ggcactgatg gtggtgttta
     5401 cgtattcatg cgcgaagcga aagtgtatta tcgcacaaat acatttatcg attggctgca
     5461 gcatggtaat gacctgccat tggtgataga tccgctcaag gctattccat tttatcgcga
     5521 cgacaaaatc agattaggaa gttggaatat cggtatatgg gaagctcctt tatatgagcc
     5581 atcaacattg atagcagatt tttctgcaga gaaaccgaat ttttattgcg caggagatca
     5641 aattaaatat gtagatcatt caacagcagg tgcaggagca acatatgcat gggaatttcc
     5701 gggaggtatt ccggctactt catcagaaca aaatcctgtt gtcacctatc ctaccgaagg
     5761 cgtattcgat gcaacgttaa cgattacaga tggtgccgaa gtgcaatcga tcacaaaatc
     5821 ggcaattgtg agttcattgg ctccgtcaac attacctttt tcagaaaatt tcgagaccgc
     5881 acaatttcct gaaggatggc atggcggaac cagcggtggc tgggggatag ctgaagatgc
     5941 aagtgcatat gatgttggca cttacagcat gttttttaat aattactgga cagacttgca
     6001 aggagatcat gttgatgaaa tcaccgcaca atatgatata gatgctgcaa ttacggcaca
     6061 gttgacattc gatgttgctt atcgtcccta cggaggaatt tatatggata cacttgccgt
     6121 ttatgtgtcg aatgattgcg gtgctacatt agatctaatt tattacaaag gtggcaatac
     6181 attggcaacc ggacctgcat atactgctga tgtattttat cctgccgcag atgaatggcg
     6241 aacagaaaca attgatttaa cgccatacat agatgatgtt tcattaatgt tggtatttag
     6301 aaatatcggt tattggggac aagcaatgta tgctgataat attaatatcg ctgtagaaaa
     6361 agaaggtatc acttcgcatg aagagaatat ttcc
//
LOCUS       DAOQOU010000179         6259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_7286_length_6259_cov_5.330271, whole genome shotgun sequence.
ACCESSION   DAOQOU010000179 DAOQOU010000000
VERSION     DAOQOU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7286_length_6259_cov_5.330271"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="tpiA"
                     /locus_tag="PLM90_12495"
     CDS             <1..732
                     /gene="tpiA"
                     /locus_tag="PLM90_12495"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMZ90247.1"
                     /translation="KMYKTLPEAMETIGALTHALAVQSPKENVEVWIAPPSVILPLII
                     HNYHNRGISFGAQNCSEHKEGAYTGEISAAMIKSVEAQFVILGHSERRAYHHENDTLI
                     HKKIRAALSNQLIAIYCCGETLDQRNAGQHFDIVRKQVTEALSGFNADEMSNIVIAYE
                     PVWAIGTGVTASPEQAQEMHALIRETLRQLFDGKVADQMHILYGGSVKPGNAAELFSQ
                     PDIDGGLVGGASLVASDFTGIIYAA"
     gene            740..1573
                     /gene="prmA"
                     /locus_tag="PLM90_12500"
     CDS             740..1573
                     /gene="prmA"
                     /locus_tag="PLM90_12500"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HMZ90248.1"
                     /translation="MEYLEYNFTTSDEQTAEILIALLADKGFDTFDHNDVQLSAYIRK
                     EDHLESAIAHFLNDLQDRFVFTWSFQAIAPKNWNEEWEKNFEPIRVGKDCLIRAPFHE
                     ADPDVTYDIIIEPRMSFGTGHHETTFLMSQEILGLQMKGKRVADAGTGTGILAILAAK
                     KGAADVFAVDNEEWAWRNAIDNIAINGVIDSVHAELGGLDLMEGKTFDIILANINKHI
                     ILEHIQLFFRCLQPGGLLIVSGMFIRDLQDVEKEAARYFFQLISTQVRNDWVCAVFQN
                     V"
     gene            1684..6159
                     /locus_tag="PLM90_12505"
     CDS             1684..6159
                     /locus_tag="PLM90_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90249.1"
                     /translation="MKYCGLLLIAISLWCTGYSQVTTFSTDPVQFMKDLDDFMKANNN
                     EIGKDSYNRFAKELEATNITAEELDLVITLCNKMKERNMKPTPQFVAVLDATVAFAKS
                     GQLFDRYAEWMEITNAILDKSKKGSYVTYNRFLEFSSDFFSKNALNATPTKSWCYTSF
                     EYQFILNGDVPEVHFPETGLYAFTKGDTMNISATKGVYYPLEKEWRGSDGYVTWARAG
                     LDSSIAYGKLRKYIVNTESNEYIADSATLYFGGLFGKPLIGQLKDHLTTNNEVDKSDY
                     PQFRSYDHELVVKNIMTNVDYKGGFLLKGRQIIGSGSDSIPAVMTFKDPKGKEALVAK
                     SQVFLVEPETSIYSTDAEVMIRVAKTDSIYHHDASIRYDGMLGTVNITRQDEGFSSLM
                     YYSSYHKVDAKVDNLSWTLGDTIIKMKNITGAGNKMSWYESQDLFSAKLFDRVRGVSS
                     YQPLVKIKRYCETYKTRVIPTLALAKELNPSLTVDGVQSLLLQLVEEGFIYYDKEHEL
                     VYVKDKTINYVLASAGKKDYDIINIPSVTKGSNSELNLVNYDLTINGIRQFYLSDSQF
                     VLIYPSNDQITMKKNRDMLFDGTIVSGNIDFKGNNFYFSYDTFSIKMNKVDSMILYVE
                     STAIDDNGNPLYLPVNTNISVSSGRLQIDKTDNKSSKVRYPEYSIFTTYDYSVADYDK
                     PNVFNNVYDKEAFYFKLDPFTLESSDSLDYRKVKFDGTMISAGIFPDFREGLKLQEDR
                     SLGFKTVTPPGGYPTYGKTGKYNGNIYLSNAGFRGSGTIDFMASTSESKDILFFPDST
                     IAKVDDFVVKKTESGIQFPAVTNSGVSTRWLPYEDQMIVSQGTAPFNMFENQATLKGT
                     AIVTSKGLRGDGIMNWGEVKLTSNQFKYLANNAKADTSAVTIGQVTPDLVALKLDNVN
                     SDLDFKERIGHFKANSDTIMTELPYNEYKTDMNVFDWIMDDRVVNFKSTKEYANFYST
                     NDGQDSLMFQAKAGKFDLNTYLLKAEGVPLINIADVQVIPDSGKVQIEKGAKMGTLYY
                     AKIKGDTLNYWHYLDNCVVNISGRLQMSGTGNYTYESPQGKKQNIPIESMNVFKVRDT
                     IMEVTVAHVLAKGYVQDTANFELENKVLFKGPVELYSNEKFLKYRGYAKLVVTDTANI
                     RTSWFHIDTPIDPKNPVFNLNDAVTERDDSVYAGIFRFNDTAALYTNIMGKRASMGDY
                     MVFSARGKGYYNETDQTFYFGNLDKLNDPTLPGSIMKYNDKTGALFCEGRLRMGLKTD
                     MCDTKAYGTIIKRPNNNNYRMEATVTFDIPLPEELMNKLGDMLLASNNDAGAAPVDYN
                     IEQQITSAVLDLLDEKSMNKAMSDITSKGYLTRPKDCPASIFVSGLKLIWDPATRSFH
                     STSGDGKLLWFGDKSFNQQIKCYAEFGKRSGGDYFTLYFQTPSEDFVYISYKRNQLKL
                     YTSRDDLNGEIFSYDGNKRIIETPKGNMVFSLEGKNAVSQFVQDMQYYESLKENK"
BASE COUNT         1977 a         1214 c         1361 g         1707 t
ORIGIN      
        1 aagatgtata aaacacttcc tgaagcgatg gaaaccattg gtgcactgac acatgcttta
       61 gcagtgcaat cgccaaaaga aaatgttgaa gtttggatag ctcctccttc agtgatatta
      121 cctttgatta ttcacaatta tcacaatcgt ggaatcagtt ttggtgcaca aaattgcagt
      181 gaacataagg aaggcgcata taccggagaa ataagtgctg cgatgattaa aagtgtggaa
      241 gcacaatttg tgattttagg tcacagcgaa cgcagagcct atcatcatga aaatgacaca
      301 ctcattcata aaaaaattcg cgctgcatta tccaatcaat tgatcgccat atattgttgt
      361 ggtgaaacat tggaccaacg aaatgccggt cagcattttg acatcgtacg caaacaagtt
      421 accgaggcct tgagcggatt taatgccgat gaaatgtcaa atatcgttat cgcatatgaa
      481 ccggtttggg caattggtac aggcgttacc gcttctccgg aacaagccca ggaaatgcat
      541 gcacttattc gcgaaacatt acgacaatta tttgacggaa aagtagctga tcagatgcat
      601 atactttatg gtggcagtgt aaaaccgggt aatgcagctg aattattttc acagcccgat
      661 atagacggcg gtcttgtagg tggtgcgagt cttgttgcat cagattttac aggtattatt
      721 tacgcagcct gattgcatca tggaatacct cgaatacaat ttcaccacgt cggacgagca
      781 aactgctgaa atattaatag cattacttgc cgacaaggga tttgatactt tcgatcacaa
      841 tgatgttcaa ttaagtgctt atatccgcaa ggaagaccat ctggaaagtg cgattgcaca
      901 ttttctcaat gatcttcagg acaggtttgt atttacctgg tcatttcagg caatagcacc
      961 aaaaaactgg aatgaggaat gggaaaaaaa tttcgagcct attcgtgtcg gaaaggattg
     1021 tctcatcaga gcaccattcc acgaagctga tccggatgtc acctacgata tcatcatcga
     1081 gccacgcatg agcttcggca ccggacacca tgaaacaaca tttttaatgt cgcaggagat
     1141 actgggactt caaatgaagg gcaagcgcgt tgcggatgcc ggcaccggaa caggaatatt
     1201 agccatttta gctgccaaaa aaggagccgc cgatgtattt gccgttgaca atgaggaatg
     1261 ggcctggcgg aatgccattg ataatattgc gatcaacggt gtaatagaca gtgttcatgc
     1321 tgaattgggc ggattagacc tgatggaggg caagactttt gatataatat tggcaaacat
     1381 caataaacac atcattttgg agcatatcca actgtttttc cgctgtttgc aaccgggagg
     1441 actgctgata gtgagcggca tgtttatccg cgacttacaa gatgtggaaa aagaggctgc
     1501 caggtatttc tttcaattaa tttccacaca agtgcgcaac gactgggttt gcgccgtttt
     1561 tcagaatgta taggcacttt agcccataat tcaggcattt aatttgtgtt aatcccatac
     1621 tgtaaagggg ttatctacgt tttgaatacc gcaggtattc cctgtgtact ttaaattaac
     1681 ggcatgaaat attgtggact tctccttatc gcaatcagtc tttggtgtac cggctattcg
     1741 caggtcacca ccttttctac tgatcctgtg caattcatga aagatctgga cgatttcatg
     1801 aaggcaaaca acaatgagat cggaaaagac agttataatc gctttgccaa agagttggaa
     1861 gccaccaata tcactgccga agaactcgat ctggtgatca ccttgtgcaa taaaatgaag
     1921 gagcgcaata tgaagcccac tccgcaattt gttgccgtat tagatgcgac tgtagccttt
     1981 gcaaaatccg gacaattgtt cgatcgctat gccgagtgga tggagatcac caatgccatt
     2041 ctcgataaat cgaaaaaagg aagttatgta acctacaatc gcttcctgga attctcgtct
     2101 gattttttct ctaaaaatgc actgaacgca acgccgacaa aatcatggtg ctatacctct
     2161 tttgaatatc aattcatttt gaatggcgat gtaccggaag tgcattttcc tgaaacagga
     2221 ttatatgcat ttaccaaagg tgataccatg aatatcagtg ccaccaaagg tgtgtattat
     2281 ccgctcgaaa aagagtggcg tggcagcgat ggttatgtta catgggcacg cgccggactc
     2341 gatagctcta tcgcttatgg aaaattgcgc aaatatattg tgaatacaga atctaatgaa
     2401 tatatcgccg attctgcaac cttatatttc ggcggacttt tcggaaagcc attgattggt
     2461 caactcaaag atcacctcac aacgaataac gaagtagaca aaagcgatta tccgcaattc
     2521 aggtcatacg atcatgaact ggttgtaaaa aacatcatga caaatgttga ttacaaaggt
     2581 ggatttttac tcaagggtcg ccaaatcatt ggatccggaa gcgatagcat tcctgctgtg
     2641 atgacattca aagatcctaa agggaaggaa gcgttggtag ctaaatcgca ggtatttctg
     2701 gttgagccgg aaacttcgat ttattcaaca gatgcagaag tgatgatacg tgttgcgaaa
     2761 accgattcaa tttatcacca tgatgcctct attcgctatg atggcatgtt ggggactgta
     2821 aacatcacaa ggcaggatga aggattttct agccttatgt actatagttc ctaccataag
     2881 gttgatgcaa aggttgataa tctcagttgg acactcggcg ataccattat caaaatgaaa
     2941 aacatcaccg gcgcaggaaa taaaatgagc tggtatgaaa gtcaggattt attcagtgcg
     3001 aaattatttg atcgcgtgcg aggtgtatcc tcatatcaac cgctggtgaa aataaaacgc
     3061 tattgcgaga cctataaaac acgtgtgatt ccgactttgg cgctggcgaa agaactcaac
     3121 cctagcctta ctgttgatgg cgttcaaagt ttattgttgc aattagtgga agaaggattt
     3181 atctactacg acaaggaaca tgaacttgta tatgtaaagg ataaaaccat caattatgta
     3241 ttagcttctg caggaaaaaa agattacgac attatcaata ttccatcagt taccaaaggt
     3301 tctaactcag agctcaacct ggtgaattac gatttaacca tcaatggtat ccgtcaattt
     3361 tatctcagcg attctcagtt cgtgttaatt tatcccagca atgatcagat caccatgaaa
     3421 aaaaatcgcg acatgctgtt tgatggcacc attgtttccg gtaacataga tttcaaagga
     3481 aacaatttct atttcagcta cgacaccttc agcatcaaaa tgaacaaggt ggactccatg
     3541 atcctttatg ttgaaagcac tgcaatcgat gataatggca atccacttta tctgccggta
     3601 aataccaata tcagtgtttc gtcaggtcga ctgcaaattg ataaaacgga taacaaatcg
     3661 agcaaggtgc gctatcctga atattccata ttcaccactt atgattattc agtagcagat
     3721 tacgacaagc caaacgtatt taacaatgtg tatgacaaag aagcattcta tttcaaatta
     3781 gacccattta ctttagagag ttccgattca ctggattatc gaaaggtcaa gtttgatgga
     3841 acgatgatct ctgcaggtat attccccgat ttcagagagg gattaaagct acaagaggac
     3901 aggtcattag gttttaaaac ggtgactcct ccgggtggat atcctactta tggaaaaacc
     3961 ggtaaataca atggcaatat ttatctcagt aacgccggtt tcagaggatc gggaacgatt
     4021 gattttatgg cttctacttc tgaatctaaa gacatcctgt ttttcccgga ttcaaccatt
     4081 gccaaagttg atgattttgt tgtcaagaaa actgaaagtg gaatacaatt tcctgctgta
     4141 acaaatagcg gtgtgagcac caggtggtta ccatatgaag atcagatgat tgtttcacag
     4201 ggaacagcac catttaatat gtttgagaat caagccacat tgaagggaac agcaattgta
     4261 acatcaaaag gcttgcgtgg cgatggcatc atgaactggg gagaagtaaa actcacttcc
     4321 aatcaattta aatatctcgc taataatgcc aaagccgata cgtcagcagt taccatcgga
     4381 caggtaacgc cggatctggt agcgttgaag cttgataatg taaattcaga tttagatttt
     4441 aaagagcgca ttggacactt taaggcgaat tcagatacca ttatgactga attaccatac
     4501 aacgaataca aaacagatat gaatgtattt gattggataa tggacgaccg cgtagtaaat
     4561 ttcaaatcga caaaagaata tgccaacttc tattcgacca acgatggtca ggacagctta
     4621 atgtttcagg ccaaggcagg taagttcgat cttaatactt acttgttaaa agctgaaggc
     4681 gttccattaa tcaatattgc cgatgtacag gtgattccgg attcaggaaa agtgcagatt
     4741 gaaaaaggtg cgaagatggg aacgctgtat tatgccaaga tcaagggcga tactttgaat
     4801 tactggcact atcttgataa ttgcgtggta aatatcagcg gacgtttaca aatgagtgga
     4861 accggtaatt acacttacga atcaccacaa ggtaaaaagc aaaacattcc tatcgagagc
     4921 atgaatgtat ttaaggttcg cgataccatc atggaagtta ctgtagctca cgttttggca
     4981 aaaggttatg tgcaagacac agcaaacttt gagttggaaa acaaagtact gttcaaggga
     5041 ccggtagaat tatattctaa tgaaaaattt cttaagtatc gtggttatgc aaaacttgtg
     5101 gtaaccgaca cagcaaatat tcgcacaagc tggttccata tcgacactcc aattgatccg
     5161 aaaaatccgg tattcaattt aaatgatgcc gtaaccgagc gcgatgactc agtatatgcc
     5221 ggaattttcc gatttaatga tactgcagca ttgtatacca acattatggg taaacgcgcc
     5281 agcatgggtg attacatggt gttctctgct cgcggaaagg ggtattataa tgaaacagat
     5341 cagacatttt atttcggcaa cctcgataaa cttaacgacc ctaccctgcc gggctctatc
     5401 atgaaataca atgataaaac cggtgcctta ttctgcgaag gtcgcttgcg catgggtttg
     5461 aaaaccgata tgtgtgatac taaggcttat ggaaccatca tcaaacgtcc aaacaataat
     5521 aattatcgaa tggaagctac cgttacattc gatattccat tgccggagga attgatgaat
     5581 aaacttggcg atatgttgct cgcatcaaac aatgatgcag gcgcagctcc agtagattac
     5641 aatattgaac aacaaatcac ctctgccgtt cttgatcttt tagatgaaaa atcaatgaac
     5701 aaagcaatga gcgatatcac atctaaaggt tatctcacac gtcctaaaga ttgccctgca
     5761 agtattttcg tatcagggtt gaaactcatt tgggaccctg caacacgatc attccacagc
     5821 accagcggtg atggcaaatt actttggttt ggcgataaat cttttaacca acagatcaaa
     5881 tgttatgcag aattcggtaa acgcagcgga ggggattatt ttacacttta tttccagact
     5941 ccttcagaag atttcgtgta tatcagttac aaacgcaacc aacttaaatt gtacacctct
     6001 cgcgatgatt tgaatggtga aattttcagc tacgatggca ataaacgtat catcgaaaca
     6061 cctaaaggaa atatggtgtt cagcctggaa gggaaaaatg cagtttcaca atttgtgcag
     6121 gatatgcaat actatgagag tctgaaagag aacaaatgat gagggaggag aaaggagaga
     6181 ggagagagga aatgtgagtg gtgagtgggt gagtttacct cgccgagtag gcgggtgggt
     6241 gagtttacct cgccgagta
//
LOCUS       DAOQOU010000180         6151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_7499_length_6151_cov_5.811024, whole genome shotgun sequence.
ACCESSION   DAOQOU010000180 DAOQOU010000000
VERSION     DAOQOU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7499_length_6151_cov_5.811024"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1212
                     /locus_tag="PLM90_12510"
     CDS             241..1212
                     /locus_tag="PLM90_12510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90250.1"
                     /translation="MVSRTEAQKNVPGNGAIWTYTIDDVDLTHGTCFYRFKYVLYADI
                     DSTINGYNYTLLKLKQTLYYTFDPFNQFYCSIDTFDHDLGIFGGIRSLDEKTYFYGLN
                     NPLQIILGLDVPDTTDVLVYDFSKIPGDTLPRKDYYGYIMYDSIKETSYVTLADGVIR
                     KQQTVVYKSYTVGISGDTILANSDQNIYVEGIGDIENGLFFPISPQYSSYYVSLNCYK
                     ENEIYLLGDNSCENEDAQFETPTLEGKTIIAYPNPFKEYFRYQVPYDHSTIRLYTLDG
                     KLVLEQVTDLPNGYISTRHIPNGAYIVTCTASDGKVAYDIICKAATY"
     gene            1284..1895
                     /locus_tag="PLM90_12515"
     CDS             1284..1895
                     /locus_tag="PLM90_12515"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ90251.1"
                     /translation="MGTRINAVDQYILKSQAFAQPILNHLRDLVHTVCPEVEEKIKWG
                     MPFFDYKNDMLCHMASFKAHAVFSFWKAQLMKDPILMENANSEQAMGHLGRITSLKDL
                     PSDKQLRAWIKEAMVLNDKGIKIVKPKSTGEKPTVEIPEMLIKALKKNKSAWAVWHKA
                     TPGYQKEYANWISDAKTEATQIKRTNTAVEWIAEGKIRNWKYQ"
     gene            complement(2207..5257)
                     /locus_tag="PLM90_12520"
     CDS             complement(2207..5257)
                     /locus_tag="PLM90_12520"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ90252.1"
                     /translation="MKKLSLILSLLLTLKLQQSVYAQQASYWQQHVDYEINVVLDDVN
                     HMLNGSIAMRYTNNSPDVLNEIWIHLWPNAYKDNSTAFAKQKLESGDVKFHFADESER
                     GFIDNLNFKVNGEEVTLTYDEETPDIAKITLNKPLSPGQTINIKTPFRVKVPNCFSRM
                     GHDEQQYLITQWYPKPAVYDRKGWHAIPYLDQGEFYSEFGNFHVFITVPKNYVVGASG
                     DLLTASEVTFLDSIAAVTATKTFETKDLSFPPSSPTTKTLEYMLKDAHDFAWFADKRF
                     NVMKSSVTLPESERVVTTYAYFNDEHGQEWLKAAEYVDRAVAFYSDNVGEYPWNVAQA
                     VDGSLEVEGAGGMEYPTITVITGKFDAKMLDNVITHEVGHNWFYGILGSNEREHGWMD
                     EGMNTFYENRYMATYYEPANQLGIPNFALAALNVDTSDINETLWHINQTTQKQNRYQP
                     VDLHSKYYTSANYGLVMYMQTGYDFRYLEDVLGTDKYDQIMRKYYRQYQFKHVYPEDM
                     QAVFESETGDSLGWFFQQLLQSDRGPDYSIKHLLKSKTAMAVTVKNNSDIPAAFTIAL
                     MSGDSIIRTDWFRGFTGSQTVYLNYNENMHVTALRIDARKKMPETERENNTIKTSGLF
                     KRVEPLQVRLFGFGADNPNRTTISVMPLVGWNDHDRWMLGLGIWNSTMPTPKIEYVLA
                     PMYSIYSGEPVGQGSVGLNFYPDNGLFDRIRISESAARYTYDEFYINALFDQAQYKPQ
                     YLKLESKIEADLHPKSMRSKLTQRLSARSIFIQEDDLYFLYDINGDITGTASASRMEY
                     ELGYHLAYDRTFFPQKVDALLTAGEGFTKLSATYHTTIHYPGMKKGMNVRLFGGAFLQ
                     DGGDENQSFTLAGNNGYYDATYDDVFLGRNESSGFLSTQMTATDGFFKVPVYNYAFTS
                     RSYMGAANFELTIPKVPLVSLFADAGYFGLRESVTGISGFQYDAGLKIGLPLDIFSLY
                     LPFVMSSDLADQFADDASYGEKMSFQLSINALNVFEMMRKLAVE"
     gene            5390..>6151
                     /locus_tag="PLM90_12525"
     CDS             5390..>6151
                     /locus_tag="PLM90_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ90253.1"
                     /translation="MMDHDFNEQDPTAIAELIEQFERYLKDNEYAYFDEESLELILEY
                     YEMRNLNERAEAVIAFALEQNPFSSDFLVRKAEYLLNRKRYQEAMDTLDKAMLFDSRE
                     IDIYLLRSDIYAEQNKLTEAEAILLEALEIADEEDKDAVYAELSDIYEIQEQFDKAFD
                     CLAKALSLNPSSEEALYKLAHIVDMTDRYDDSVSIHTGIIDKDPYAWLAWYNLGRAYI
                     GLGLYEKAMEAFEYVMAIDEHFDLVYRDAADLYYRM"
BASE COUNT         1866 a         1310 c         1200 g         1775 t
ORIGIN      
        1 ccgctattat tcgatagaat gcctaaggct ttcaattcgg cagccatggc atttccctgt
       61 atcattgtat ccatttagct atatttaggg tgcaaaatta cgaaatttcg gggtgaatga
      121 accccgttga actgacctag acagtatcgt cataaggcgt attcaacagt gctttaccga
      181 ttaaactaat attaaatccg tttagttcga atttatattc tattacttac attaaccggt
      241 gtggtatcac gtactgaagc acaaaagaat gttccgggca atggtgcaat atggacttat
      301 accattgatg acgtcgattt aactcacggc acttgtttct atcgctttaa atatgtgctt
      361 tatgcagaca ttgacagcac tattaatgga tataattata cacttctaaa attaaagcaa
      421 acgttatatt acacttttga tccatttaat caattctatt gctccataga tacttttgat
      481 catgatttag gaatatttgg tggaatacgg tcacttgatg agaagacata tttctatggg
      541 ttgaacaacc ctctacaaat tatcctcggt ctggatgtcc cggatactac cgacgtattg
      601 gtttacgact tctctaagat accgggagac accttaccac gtaaagatta ttacggatac
      661 attatgtatg actccattaa agaaactagc tatgtaacac ttgcagatgg agtcataaga
      721 aaacagcaaa ctgtcgttta caagtcctat actgtcggaa tctcaggcga tacaattctt
      781 gccaatagtg atcaaaacat ttatgttgaa ggcattggag atattgaaaa cggattattt
      841 tttccaatct caccccaata ttcaagttat tacgtaagtt taaattgcta taaggaaaat
      901 gaaatatatt tattgggtga caatagttgt gaaaatgaag acgcacaatt tgaaactcct
      961 actttagagg gaaaaacaat aattgcatat cctaacccat tcaaggaata ttttagatac
     1021 caagtgccgt atgatcattc taccatccgc ttgtatacat tagatggcaa gcttgtatta
     1081 gagcaggtga ctgatttgcc aaatggatat attagtactc gccatattcc aaatggcgct
     1141 tatatcgtaa cttgcacagc tagcgatgga aaagttgcat atgacattat ttgtaaggcc
     1201 gcaacatatt aaatacctcc aaaatttatt aaatacaatt aatatcaaca gatcaattta
     1261 ataaactctg aaaaatcaac caaatgggca cccgaatcaa cgccgtagat caatacatcc
     1321 taaaatcaca ggcattcgct caaccaatat tgaatcacct tcgtgatctg gtgcataccg
     1381 tttgcccgga ggttgaagaa aaaataaaat gggggatgcc atttttcgat tacaagaatg
     1441 atatgctgtg tcatatggca tccttcaaag ctcatgcagt ttttagtttc tggaaggcgc
     1501 aattgatgaa agatcccata ttgatggaaa atgccaattc ggagcaggcc atgggccatt
     1561 taggtcgcat aacctcgtta aaagaccttc cttccgacaa gcaactaaga gcctggatca
     1621 aagaggcaat ggtgctgaat gataagggca taaaaattgt aaaaccgaaa tcaacaggcg
     1681 aaaaacctac tgtcgaaatt ccggaaatgc tgatcaaagc acttaaaaag aataaatcgg
     1741 catgggcagt atggcataag gctactcccg gatatcaaaa agaatatgcc aactggatat
     1801 cagatgcaaa aacggaagcc actcagatca aacgaaccaa tactgcagtt gaatggatag
     1861 cagaaggaaa gatccgcaac tggaaatatc aataaaggaa agaggagaga ggagagagga
     1921 gagaggagaa aggggtgagt aggtcagtgg gtgagtgggt gagtgggtga gtgggtgagt
     1981 gggtcagtgg gtgagtgggt cagtgggtga gtgggtgagt gggtgagttt acctcgccga
     2041 gtaggcgggt gagttagtag gcaaataaaa tgggctaaga catagtctta gcccatttta
     2101 tttgaatcct ccgtaccaca ctcacgattc acgagtcacg attcacgatt cacgatctac
     2161 taccgcctat cggcgaggca agcccgccaa atgcgaggca agctcactat tctaccgcca
     2221 acttgcgcat catttcgaat acattcaatg cgttgatgct aagctggaac gacatttttt
     2281 ctccatagga ggcatcatcg gcaaactgat cagcaagatc gctgctcatg acaaatggaa
     2341 gatatagact gaagatatct agaggcagtc ctatcttcaa accggcatca tactgaaatc
     2401 ctgagatacc tgtcacactt tctctcaacc cgaaataacc tgcatctgca aataatgata
     2461 ccagaggtac tttaggaatt gtcaattcaa aattcgctgc acccatataa ctcctggatg
     2521 tgaatgcgta attatataca ggcactttaa agaatccatc agtagctgtc atttgcgtgc
     2581 taaggaaacc agacgattca tttctaccta gaaaaacatc atcatacgtt gcatcataat
     2641 atccgttatt tcctgctaaa gtgaatgact ggttctcatc accaccatct tgtaaaaatg
     2701 ctcctccgaa taaacgaaca ttcatacctt ttttcattcc gggatagtga atggtagtgt
     2761 gatatgttgc agaaagctta gtaaagccct cccctgccgt taacaatgcg tcaacttttt
     2821 gcgggaaaaa tgttctgtcg taagctaagt ggtatcctaa ttcatattcc attcgcgaag
     2881 cagatgcagt tcctgtaata tcgccgttga tatcatataa aaagtataag tcatcttcct
     2941 gaataaatat tgacctggca cttaagcgtt gtgtgagttt tgatcgcatg gatttaggat
     3001 gcagatctgc ctctattttg ctttccaatt ttaaatattg aggcttgtat tgcgcctggt
     3061 caaacaaggc attgatataa aattcatcat aggtatatcg cgctgcactt tcagaaatgc
     3121 gaatcctatc gaacaaaccg ttatccggat aaaaattcaa tccaacgctc ccctgaccta
     3181 caggctcacc ggaataaatg ctatacatag gcgccagcac atattctatt ttaggcgttg
     3241 gcatggtact gttccatatt cccaagccta gcatccatct atcatgatcg ttccatccta
     3301 ctaatggcat tacacttatt gtggtgcgat tcggattgtc ggcaccgaat ccaaacagcc
     3361 ttacctgcaa aggttccact cgtttaaaca agcccgatgt ttttatggta ttattttcac
     3421 gttccgtttc aggcattttc tttcttgcat ctatgcgcaa tgcagtaaca tgcatatttt
     3481 cattgtaatt caaatatacc gtctgactac ctgtgaatcc gcgaaaccaa tcggtgcgga
     3541 taattgaatc tcccgacatc aaagcaatcg taaatgccgc aggaatatca ctgttgtttt
     3601 tcacggttac agccatcgct gttttgctct ttaataagtg cttgatactg taatccggcc
     3661 cgcgatcact ctgcaacaat tgctggaaaa accatcctaa cgaatcaccg gtttcagatt
     3721 caaacacggc ttgcatatct tcaggatata cgtgtttaaa ttgatactgg cgatagtatt
     3781 tgcgcatgat ctgatcatat ttatccgtgc caagtacatc ttccaaataa cggaaatcat
     3841 aaccggtttg catatacatt accaaaccat aattcgcact tgtataatat ttcgaatgca
     3901 aatcaaccgg ttgataacga ttttgttttt gagtagtctg gttaatatgc cataatgttt
     3961 catttatatc tgatgtatca acatttaatg ccgctaatgc aaaatttggt atcccaagct
     4021 gatttgcagg ctcataatag gtagccatat atcggttctc ataaaatgta ttcatccctt
     4081 catccatcca accatgctca cgctcattgc ttccaagaat tccataaaac caattatgcc
     4141 caacctcatg cgtaattaca ttatccagca ttttcgcatc aaattttcct gtgataacag
     4201 taatggttgg atattccatt ccaccggcac cttcaacttc cagcgagcca tcaactgcct
     4261 gcgcaacatt ccatggatat tcacccacat tgtcagaata aaatgctact gctctatcaa
     4321 catactctgc agccttcaac cattcttgtc catgctcatc attaaaatat gcataagtgg
     4381 ttaccactcg ctccgactcc ggaagcgtaa cactgctttt cattacatta aatcgcttgt
     4441 cggcaaacca tgcaaaatca tgcgcatctt tcaacatgta ttccaatgtt ttggttgttg
     4501 gtgaagaagg cggaaaactc aaatctttag tttcaaatgt ttttgtagcc gttaccgcag
     4561 cgatactatc taaaaatgtc acttcacttg cagtcagtaa atcaccactc gcgccaacaa
     4621 cataattttt tggaacagta ataaatacgt ggaaatttcc gaattcactg taaaattctc
     4681 cctggtcaag atatggaatg gcatgccagc ccttgcgatc gtatactgcc ggtttcggat
     4741 accattgtgt gatcaaatat tgctgttcat catgacccat gcgcgaaaaa caattcggca
     4801 ccttaacacg aaatggcgtt ttgatattaa tggtttgtcc cggcgataat ggtttattca
     4861 aagtaatttt tgcaatatcc ggcgtctctt catcataggt aagtgtaact tcttcaccat
     4921 tgactttaaa gttgagatta tctatgaagc cacgttcaga ctcatcggcg aagtgaaatt
     4981 tcacatcccc cgactctagc ttttgtttag caaatgccgt gctgttgtcc ttatacgcat
     5041 ttggccaaag gtgtatccaa atctcgttta atacatcggg tgaattattc gtataccgca
     5101 tagcgatgct gccatttagc atatgattta catcgtcgag cactacattg atctcgtaat
     5161 ccacatgctg ctgccaataa cttgcttgct gagcatatac agattgttgt aacttgagcg
     5221 tcaatagtaa agagagaatc agggagagtt ttttcatcct tgtagaattt ttgtaaagat
     5281 aaggttcagt tttctgcttc ctaaatgagg caaaagccca aaatgttaca aaatgcctct
     5341 tttttgagat tcaggaaact attcaggtca tttgtaaatt tgcaataata tgatggacca
     5401 tgattttaac gaacaagacc ctacggccat tgcagagttg atcgaacagt tcgaaaggta
     5461 cctcaaagac aatgaatacg cctatttcga cgaggagagc ctggagctga tcctcgaata
     5521 ctatgaaatg cgcaacctca acgagcgcgc cgaggctgtg attgccttcg ctttggaaca
     5581 aaatcctttt tcctccgact ttctggtccg caaggcggag tacctcctca acagaaaacg
     5641 ctatcaggaa gccatggata ccctcgacaa agccatgctc ttcgatagtc gcgagatcga
     5701 tatctacctt ttgagatcgg atatctatgc cgaacaaaat aaacttacag aggcagaagc
     5761 tatcctgctg gaagcccttg aaatagccga tgaagaggat aaagacgccg tatatgccga
     5821 acttagcgat atctacgaga ttcaagagca gttcgataag gcattcgact gtctggcaaa
     5881 agcattgtcc ctgaacccgt cctctgaaga agcactctat aaactggccc atatcgtaga
     5941 tatgaccgac aggtatgatg attctgtttc gatacataca ggtatcatcg acaaagaccc
     6001 atacgcctgg ctggcatggt ataaccttgg tagagcctat atcggactgg gactttacga
     6061 gaaggctatg gaggcttttg aatatgtaat ggccatagac gagcatttcg accttgttta
     6121 ccgcgatgcc gctgacctgt attatcgcat g
//
LOCUS       DAOQOU010000181         6147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_7504_length_6147_cov_6.164150, whole genome shotgun sequence.
ACCESSION   DAOQOU010000181 DAOQOU010000000
VERSION     DAOQOU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7504_length_6147_cov_6.164150"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3523
                     /locus_tag="PLM90_12530"
     CDS             <1..3523
                     /locus_tag="PLM90_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ90254.1"
                     /translation="ISNTTGGEPWYTVSNSQSMDAVFGVGEWTMGYFETVDPAVVFGT
                     GTCFVFLEGSDGHALELKSFLDANHTAIENWVASGGHLLLNAAPNEGSGMDFYFDGVE
                     LSYPYFSNTVGTDVPTHPAFTGPFAPTSTVMYGSSYGHATVSGDDLSVILYDYYNPSK
                     IVLAEKSWGAGTVLFGGMTVTNYHSPLPDADNFRRNAISYLATCTISDNDMATTAILS
                     PESTCGLTGAETITINVKNVGFLSQTDVPVSYQVDGGTIVTETIPGTIDVGEIVTYSF
                     ATTVNMAVPGDYNIVTWTDLATDTIYGNDTTYSTVYNVPTVASFPYYEDFEDGSKGWL
                     VGGASTFELGTPAGYVINTPPTATPGSLNSWVTNLEGYYDNNEKGYVTSPCMDFTSLV
                     MPYMELDINYDIQLYSDGAKIQYTLDEGASWSDLGNVGEGENWYDSYYCYSMWPTFYV
                     SDYRGWQSTYTGWRTAHYDMSFLAGQPSVRFRIIFASDSYWNYNDGFAFDNVKIQDPF
                     PNDVGVVDLISPESAVTLTGSEVVSVLLENFGTLPQTGFNVAYKVGAGTVHTELFTGT
                     VDPGSTAVMTFAATEDFSADGLYNVTAWTELASDEDLTNDTLNAIVVNLTPVSGTDAF
                     YIYSNVYGGYEPWYVTSNSDAMDAVFGDDGWFLEYVETLDPYAVFDENTCFVYLEGSD
                     AQASELENFLTTNSSIVENWVASGGHLLLNSAPNEGDGMDFGFDGVTLNYSWYTSNVT
                     AYDPSHPIFNGPWTPITTDYTGGSFGHASVSGGGIDPVIIDTYDASRYVLAEKEWGDG
                     HVIFGGMTPPYFHSPSPDAQNLLQNIYETIKLCAPVDLGVVEIISPEGGCGMGVETIT
                     VTVENFGPSTVSTFPIKYQVDGGAIESDFATVTITPGGTATYTFDVSYDFSVPGTYDL
                     CVWTDFAGDSDEGNDMICVTITSLATPSVDLGPNMTVCDLVTLDAGNVGSIYSWSTGE
                     STQTIDVTESGTYSVTVTNPTTGCSVTDEVTVTVNYTPEASFTYSASGLTVTFTNTST
                     DGASYSWSFGDAGTSTTINPSHTYAVTGMYTVSLTVTNPCGSDTYSMVVEVGNGVNDI
                     TLAEAVEITPNPTSDYATVQVSLPVSADIQFKLTDYLGQEVWSANMTNSLSATYVIDM
                     TKFAAGVYQLNIISDDAVVGKQIVLTK"
     gene            complement(3573..4202)
                     /gene="nth"
                     /locus_tag="PLM90_12535"
     CDS             complement(3573..4202)
                     /gene="nth"
                     /locus_tag="PLM90_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMZ90255.1"
                     /translation="MTRSEKAQFILSKLNELFPETPVPLQHKDAYTLLVAVVLSAQCT
                     DERVNQITPKLFARADNAYDMVKVPVQEIHDIIKPCGLAPMKSKGIHGLSEMIVTKHH
                     GQVPSTFAELEAMPGVGHKTASVVMSQAFDFPAFPVDTHIHRLAWRWGLTNGKSVEQT
                     ERDLKRIFPENTWNKLHLQIIYFGRKYCPARGHDREKCPICSVVGRKLM"
     gene            4328..>6147
                     /locus_tag="PLM90_12540"
     CDS             4328..>6147
                     /locus_tag="PLM90_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90256.1"
                     /translation="MRKLHTLLFLFILAGYTCSAAEIMWASKVISYSSQLDLNSYSAK
                     QVLGPPSRLPNFGDCGCAWSPSMSENFFEEYIRVGFDKRIRVSQIVINESFNAGAIKA
                     IYLFDQYNIPHLVWERPAEGNKWELGRVFSINITPTDFASNDLKLVLDTESIDGFNQI
                     DAIGISEAPIEIPRGSINVTDKVTFKGKSQNLGPTINTYGSEITPLLTPDGKTMYFTR
                     KNHMGNTGTIMNDDIWVSTYDGTKWTEATNIGGPMNNENNNYVVGISMNGEMLTLANT
                     YSPSGESLIGIAQTWKTEYGRWVFPKNLITPGVITHGWFAEYFMNSDRDVLLLSLERA
                     DSHGMKDIYVSFSDNQIEWTEPINLGTDVNTASSEMAPFLGPDGKTLFFSSDGLPGYG
                     DQDVYVCVRLDSTWKHWTKPENLGSLVNSKGFEAYFTYPDTADYAYFSSTGDDKLNAD
                     IYRIPLKEVKEIEDSVLAEILSDVVLTSGDSTVADIITPDKPADPGEILFEEEVVVPE
                     KEALNITLTNEILLFGTVFDANTNVPINAKLSFVLTDYESDPIDMNTLNNTYRLKVTD
                     SVQYKVTVIREGYLPLEMTINIEDFRTQKVKRVDFKLTPL"
BASE COUNT         1717 a         1333 c         1306 g         1791 t
ORIGIN      
        1 catttctaac accacaggcg gtgaaccttg gtatactgtg agcaactcac aatctatgga
       61 tgccgttttc ggtgttggtg aatggaccat gggctacttc gaaaccgttg accctgcagt
      121 ggttttcggc acaggaactt gttttgtgtt cctcgaaggt tctgatggac atgcactgga
      181 actcaaatca ttcctcgatg ctaaccatac tgcaattgaa aactgggttg cttccggtgg
      241 acacctcctg ctcaacgctg caccaaatga aggttcaggc atggatttct atttcgatgg
      301 tgtagaactt agctatcctt atttctcaaa tacggttggt accgacgttc caacccaccc
      361 tgcatttacc ggtcctttcg ctccaacttc aactgtcatg tatggttctt cttatggcca
      421 tgcaacagtt agcggggatg atctttctgt aattctctat gattattaca acccaagcaa
      481 aattgtattg gctgaaaaat catggggagc aggaaccgtg ctgtttggtg gtatgacagt
      541 tactaactac cactcaccgc ttcccgatgc agataatttc cgcagaaatg caatctctta
      601 ccttgcaacc tgtacgattt cagataatga tatggcaaca acagctatac tttcacctga
      661 atctacttgt ggtttgacag gtgcggaaac catcacaatc aatgtgaaaa acgtaggttt
      721 cctctctcaa actgatgtgc ctgtaagcta tcaggttgat ggcggtacta ttgtgaccga
      781 gactattccc ggtacaattg atgttggcga aattgttact tactcattcg caactaccgt
      841 aaacatggca gttccgggcg attacaatat cgttacatgg acagatcttg caacagatac
      901 catttatggt aacgatacca cttattcaac agtatacaac gtaccaacag tggcatcatt
      961 cccttactac gaagacttcg aagatggtag caaaggatgg cttgttggag gtgcatctac
     1021 cttcgaactt ggaactcctg caggttatgt gatcaacact ccgcctacgg caactccagg
     1081 ctcactcaat agctgggtta ccaatcttga aggttattat gacaataacg aaaaaggata
     1141 tgtgacctct ccttgtatgg actttacctc tctggtaatg ccatacatgg aattggatat
     1201 caactacgac attcagttat attccgatgg tgccaaaatc caatatacac ttgatgaagg
     1261 tgcttcatgg agcgacctcg gaaacgtagg tgaaggtgaa aactggtatg atagctacta
     1321 ttgctattca atgtggccta ctttctacgt ttcagattat cgtggttggc aaagtactta
     1381 taccggctgg agaacagcgc attatgacat gtcattcctc gccggtcagc ctagcgtgcg
     1441 tttcagaatc attttcgcat ccgatagcta ttggaactat aatgacggct ttgcattcga
     1501 caacgtgaaa attcaggatc cattcccgaa cgatgttggt gttgttgacc ttatttctcc
     1561 tgaatctgct gttaccttaa ccggttctga ggttgtaagc gtgttattgg aaaacttcgg
     1621 aacattacct caaacaggct ttaacgtagc ctataaagtt ggagccggta cagtgcatac
     1681 tgaattattt acaggcacag ttgatccggg ttcaacagcc gtaatgacct tcgcagctac
     1741 tgaagatttc tctgctgatg gtctttacaa tgtaaccgcc tggactgaac ttgcttccga
     1801 tgaagatctg acaaacgata ccttgaatgc tatcgttgtc aaccttacac cggttagcgg
     1861 cacagatgcc ttctatattt attctaatgt ttacggaggc tacgaacctt ggtatgttac
     1921 ctctaactca gatgccatgg atgcagtgtt tggcgatgac ggttggttcc tcgaatacgt
     1981 ggaaacactc gatccttatg ccgtattcga tgagaacact tgcttcgttt acctcgaagg
     2041 ctctgatgct caagccagtg aactggaaaa cttcctgaca accaatagca gcatcgtaga
     2101 aaactgggtt gcatcaggag gtcacctcct ccttaactca gcgccaaatg aaggcgatgg
     2161 tatggatttc ggatttgatg gcgttacctt gaactactct tggtatacat caaacgtaac
     2221 cgcttacgat ccatcacatc caatcttcaa cggtccttgg acaccaatca ccactgatta
     2281 taccggcgga agcttcggcc atgcctctgt atcaggtgga ggcattgatc ctgtgattat
     2341 cgatacttat gatgcttcac gttatgtgct ggctgaaaaa gaatggggcg atggacatgt
     2401 aatcttcggc ggcatgacac ctccgtattt ccactctcct agccctgatg cacaaaacct
     2461 gcttcagaat atctatgaaa ccatcaagct ctgcgctccg gttgacttag gtgtagttga
     2521 gatcatctca cctgaaggcg gttgcggcat gggcgttgaa accattaccg ttactgtaga
     2581 aaacttcggt ccttcaacag tatctacctt cccaatcaaa tatcaagttg atggtggcgc
     2641 tattgaatca gatttcgcaa cagttaccat cactccggga ggtactgcaa catatacatt
     2701 cgatgtatct tacgatttct cagttccggg aacatatgac ctctgcgttt ggactgattt
     2761 tgcaggcgac tctgatgaag gtaatgacat gatctgtgtt actatcactt cactcgctac
     2821 accatcagtt gaccttggtc ctaatatgac ggtttgcgac ctggtaacgc ttgatgcagg
     2881 taacgttggc agcatctata gctggagcac cggcgaaagc acacaaacta tcgatgtaac
     2941 agaatcgggc acctactctg taacagttac caaccctact acaggttgtt ctgtgactga
     3001 tgaagttacc gtaacagtga attatactcc tgaagccagc ttcacctata gtgcaagcgg
     3061 actcaccgta acattcacca atacatctac cgatggcgct tcctatagct ggagcttcgg
     3121 tgatgccgga acttcaacaa caatcaaccc ttcacatact tatgctgtaa caggtatgta
     3181 tacggtttca ttgaccgtaa ctaacccttg tggttcggat acctacagta tggttgttga
     3241 agttggaaac ggagtgaacg atatcacact ggctgaagca gttgaaatca cacctaaccc
     3301 tacaagcgac tatgctaccg tgcaagtttc tttaccggta tcggctgata ttcaattcaa
     3361 attgactgat tacctcggtc aagaggtttg gtcagctaac atgactaatt cattgtcagc
     3421 tacttatgta attgatatga ccaagtttgc tgcaggcgta tatcaactga atattatcag
     3481 tgatgacgca gttgtaggca aacaaatcgt attgactaag taaactaacg accggcataa
     3541 caaagggctg tccaatcggg cagccctttt ttttacatca atttccttcc tacaactgag
     3601 caaataggac atttttccct atcatgtcct cgggcagggc aatacttccg tccgaagtaa
     3661 atgatctgaa ggtggagctt attccaggta ttctccggga agatgcgctt gaggtctctc
     3721 tcggtttgtt ctacggattt gccgtttgtc aacccccatc gccacgcaag gcggtgaata
     3781 tgtgtatcga caggaaatgc aggaaaatcg aatgcttgtg acatcactac acttgcagtt
     3841 ttatggccta ctccgggcat ggcctctaat tccgcaaaag tggaaggcac ttgtccgtgg
     3901 tgtttggtaa ctatcatttc cgacaagcca tgtatccctt tcgatttcat tggtgccaat
     3961 ccacagggtt tgatgatatc gtggatttct tgaaccggaa ccttaaccat atcataggca
     4021 ttatccgctc tggcaaagag ttttggggtt atttgattca cccgctcatc ggtgcattga
     4081 gctgataata ccactgctac aagcaacgta tacgcatctt tatgctgcaa gggaacagga
     4141 gtttcaggga acaactcatt tagcttggaa agaatgaatt gtgccttttc tgatctggtc
     4201 atgcggacaa atttctatac agatttgcaa ttaattgctt ttacactgtt acaaattaac
     4261 cgtatttgta attttatagc ccgtcaggtt taaataattt tacgcaaacc tgaggaattg
     4321 tgacattatg aggaaactac acacattatt atttctgttc atcctggccg gttatacatg
     4381 ctctgctgcc gaaatcatgt gggcctccaa ggtcatctcc tattcgtcgc aattggattt
     4441 gaactcctac tctgccaagc aagtattggg acctccatct cgtttgccga atttcggcga
     4501 ttgcggttgc gcatggtcgc cctcgatgtc tgagaacttc tttgaggaat atatcagagt
     4561 cggatttgat aaaagaattc gcgtgtcaca aattgtgata aatgaaagct ttaatgcagg
     4621 tgcaataaaa gcgatctatc tcttcgacca atataatatt ccccatttgg tttgggaacg
     4681 tcccgccgaa ggaaataaat gggaattggg ccgtgtgttt tccatcaaca ttacacctac
     4741 tgattttgca tccaatgatt tgaagcttgt tcttgatact gaaagtatag atggatttaa
     4801 tcaaattgat gctataggta tttcagaagc accaattgaa attccaagag gctctatcaa
     4861 tgttactgac aaagtgactt ttaaaggtaa aagccaaaac cttggaccaa caattaatac
     4921 ttatggttcg gagattactc ccttgctcac gcctgatgga aaaacgatgt acttcaccag
     4981 gaaaaaccac atgggaaata caggaaccat catgaatgat gatatttggg tttctactta
     5041 tgatggcacc aaatggactg aagcaacaaa tatcggtgga ccgatgaata atgaaaacaa
     5101 taattatgtt gtcggcatat ccatgaatgg ggaaatgctt acgcttgcaa atacttattc
     5161 tccttccggt gaaagtttaa ttggtattgc gcaaacatgg aaaactgaat atggccgttg
     5221 ggtgtttcca aaaaatctta tcacaccggg tgtaataaca catggctggt ttgctgaata
     5281 tttcatgaat tccgatcgcg atgtgttatt gttgtcactc gagcgtgccg acagccatgg
     5341 aatgaaagat atttatgtaa gcttcagcga taatcaaatt gaatggactg aacctattaa
     5401 tctgggaacc gatgtcaaca cagcttccag cgaaatggcg ccattcttag gtcctgacgg
     5461 aaaaacatta ttcttttctt cagatggttt accgggttat ggcgatcagg atgtttatgt
     5521 ttgtgtacgc ctggatagta catggaaaca ttggacaaaa cctgaaaatc ttggaagtct
     5581 tgtaaattct aaaggattcg aagcttattt tacatatccc gatactgctg attacgcata
     5641 tttctcttct acgggagatg ataaactcaa tgctgatatt tatcggattc ctttaaaaga
     5701 agtcaaggaa atcgaagact cagtattagc tgaaatactt tctgatgtag ttttaactag
     5761 cggtgattca acagttgctg atatcatcac accggataaa cctgcagatc cgggggaaat
     5821 tcttttcgaa gaagaagttg tagttcctga aaaagaagca ttgaatatta cactgacaaa
     5881 tgaaatccta ttgtttggaa ctgttttcga tgcgaatacc aatgtgccga taaatgcaaa
     5941 attatccttc gttctgactg attatgagag cgaccctatc gacatgaata cgttgaataa
     6001 tacgtatcga ttgaaagtaa cagatagtgt gcaatacaaa gtgacagtga tcagagaagg
     6061 ttatttaccg cttgaaatga caattaatat cgaggacttc cgcacacaaa aagtaaaacg
     6121 tgtcgatttt aaattaacac cattaca
//
LOCUS       DAOQOU010000182         6024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_7756_length_6024_cov_5.723572, whole genome shotgun sequence.
ACCESSION   DAOQOU010000182 DAOQOU010000000
VERSION     DAOQOU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7756_length_6024_cov_5.723572"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLM90_12545"
     CDS             <1..761
                     /locus_tag="PLM90_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ90257.1"
                     /translation="RFGIGLAALTDVAGDGSLTTNKLYLSSAYHIGYTEHDAVRFAVG
                     ISGGMVQKTIDISKLYFDTQWDGFEFDTSTISNETFSTTTINYPDINIGAMLTILPYE
                     GRRIFVGASASHINKPNESFFGETNELGIKFTATAGGSFATAGVANFQPQVYVSTQKS
                     ALEVIAGSNISIPMDVEGNINNAIFIGAWYRYGDAAWLCGGFIADNFTASVSYDFNLS
                     KLRTASSTMGGLEIAAAYVFGNTGKKDPLKCPAY"
     gene            complement(774..3008)
                     /locus_tag="PLM90_12550"
     CDS             complement(774..3008)
                     /locus_tag="PLM90_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ90258.1"
                     /translation="MPKQQTHNTKAVRFPTDFELFFRISADVMMVLDIDGKVIDFNNA
                     YERISGYTREELFALNGGWDLILQDDLPKLYDLLSTFSKDRSEVLNFTLRKRMKNGTI
                     KTISYDAILNKITNHIYAIGRDITNIMQQEQRMEDTQSRLQFFFDHALDGMSIIVDGK
                     YQMVNNAFMRIFGCSSPEDVVGRNFLDFVDSEFKEKVGRIVETNSDELYTSRIIRIDS
                     GEARNVEVTGKTIQHHNQKVRISVVRDLDDRRNLQALVKAGEERFKTVFNNAALGIVL
                     TDFAGNIVESNYTFMQRHGYTWDEIRNTQFCELFHEEDKPGVVRYMEALRNGQIENAF
                     VEKRLITKNGVVAWSKMTCSTMVGAHGETLLLSLLENIDSQKRSEQALIESEVKFRAI
                     YENSPMGIVITAAPGVIIDANPAFTKMMGYSDTELFNLPIIDLVHPADAISSGNWLQK
                     IYKNEIPHYSIEKRFDRKDGTSFWAREVMSNMFSIGSISLSVAIIENIETQKRTEEAL
                     EQKNIELTNTNQELEHFAYVASHDLQEPLRTITSFIQILDKRYRNSLDEDAGQFIGFI
                     VDGAKRMQTLIHDLLEYSRVNRFNTQFEKVDLNDVFHGVSSALKDKIENNDALVMAEQ
                     LPIVTGNRIQLTQVFQNLIDNAIKFKAKRRKPEVVVSVIEHANNWELIFADNGIGISQ
                     EYFQRIFIIFQRLHTLEEYTGTGIGLAICKKIIERHGGEIWVNSKPGKGTSFHFTLSK
                     HVVH"
     gene            complement(3056..3967)
                     /locus_tag="PLM90_12555"
     CDS             complement(3056..3967)
                     /locus_tag="PLM90_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ90259.1"
                     /translation="MDPAYRKAYWAYHLCVFLWGFTAVFGRIIDLNETILVWYRMGIT
                     SAILLCIPSLWKTIRQVDKKTLLRLIGIGVLVTIHWISFYGSIKSSNISVALTCLATT
                     SFFVSVFEPLLFGKKFVWHELLLALLVIPAMYCIFHFSGNYKTGIILGLISTVFATLF
                     SLLNKKMTKVTAPLNITFIEIFTGFILLSAIMPLYLHWQPEADLQPSTMDGINLILFA
                     GICTVVPFTLSLYSMRHISVFTASITLNLEPVYGIIMAIVFFHENDHLEPGFYIGTLL
                     ILLVVFINPFIQRKFGGNVQAPLTIGE"
     gene            complement(3977..4672)
                     /gene="radC"
                     /locus_tag="PLM90_12560"
     CDS             complement(3977..4672)
                     /gene="radC"
                     /locus_tag="PLM90_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMZ90260.1"
                     /translation="METSTNYTLKHLAEEDRPREKMLAFGRQSLTVAELIAILIGSGN
                     TGETAVELSTRILAHYGNDLNAIGKLQVSDLTQFNGIGEAKAVSIIAAMELGRRHALA
                     VAELKTQVRSSNDIFENIHPHLSDLAHEEFWVIYLNKANRIISKEKVSSGGVAGTVVD
                     VKIILRLALQKLASGIILAHNHPSGNLQPSDADISVTKKLRDAAKLMEIAVLDHLIIG
                     DRDYYSFADNGIL"
     gene            complement(4764..5015)
                     /gene="rpsT"
                     /locus_tag="PLM90_12565"
     CDS             complement(4764..5015)
                     /gene="rpsT"
                     /locus_tag="PLM90_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HMZ90261.1"
                     /translation="MAHHKAAKKSIRQSEKRNEANRYYGKTTRNAIRKLRATTDSKEA
                     DKMLPEVVSMIDKLAKRNVIHKNKAANLKSSLMKKAVAK"
     gene            complement(5240..>6024)
                     /locus_tag="PLM90_12570"
     CDS             complement(5240..>6024)
                     /locus_tag="PLM90_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMZ90262.1"
                     /translation="CPSCNQAIYKPEEEAVYRCINPECPAQMEERLIHFVSKDAMDID
                     GCGRETISELYQAGKITTIPDIFDLKTSDLAGREGWKEKSIAKLIDGIEASKKQPLWR
                     LVNGLGIRHLGTQTAKDLVKNISDLTELFDWDLERYQAIEGIGPKVAASIHQYFSNAF
                     NQQMILKLKEDGLNMANEKTAGGSGKLSGKTFLFTGSLTRFTRDEAKELVEANGGKLI
                     GSVSKNLNYLVAGESAGSKLDKAKALGTVEIIDEEAFLAMIA"
BASE COUNT         1662 a         1344 c         1228 g         1790 t
ORIGIN      
        1 atcgctttgg aatcggcttg gctgcactta ccgatgtagc cggcgatgga tcacttacta
       61 caaataaatt atatctgtct tcagcatatc atatcggcta caccgaacac gacgctgttc
      121 gctttgctgt cggaatttca ggaggcatgg tgcaaaaaac tattgatata tcgaaattgt
      181 attttgacac gcagtgggat ggctttgaat ttgataccag tacaatttcc aatgaaacat
      241 tttctacaac tactataaat tacccggata ttaatatcgg tgcgatgctg acaattttac
      301 catatgaagg tcgacgcatt tttgttggcg cctctgcatc gcatattaat aaaccaaatg
      361 aatctttttt tggcgaaacc aatgaattgg gtatcaagtt cacggctacg gcaggtggtt
      421 cattcgctac tgctggtgta gccaattttc aaccacaggt atatgtgagc acccaaaaga
      481 gtgcacttga agtcattgcg ggaagtaata tcagtatacc tatggatgtt gaaggcaata
      541 ttaataacgc catattcatt ggtgcgtggt accgctatgg cgatgcagca tggctatgcg
      601 gcggatttat tgccgacaat tttacggcat cggtaagcta cgattttaac ttgtcgaaac
      661 tgcgcactgc cagcagcact atgggtgggt tggaaatcgc agcagcttat gtgttcggca
      721 ataccggaaa aaaagatcca ttgaaatgtc ctgcttatta agcaacattt ttattaatgc
      781 accacatgtt tcgaaagtgt aaagtgaaag cttgttccct ttcccggttt tgaatttacc
      841 caaatctctc cgccatgacg ctcgataatt tttttgcaga tggcaagacc tattcctgtt
      901 ccggtatatt cttctaatgt atgcaaacgc tggaagatga tgaagatgcg ctgaaaatat
      961 tcctgactga tgccaatccc attgtcggca aaaattaatt cccaattatt ggcatgttcg
     1021 attacgctta caacaacttc cggtttgcgc cgcttggctt tgaatttgat ggcattgtcg
     1081 atcaggtttt gaaatacctg tgtaagctga atccgatttc cggttactat tggcaactgt
     1141 tctgccatca ctaatgcgtc gttattttca attttgtctt tcaatgctga actgacacca
     1201 tgaaaaacat cgttgagatc cactttttca aactgtgtgt tgaagcgatt taccctgctg
     1261 tattctaaca ggtcatgtat gagtgtttgc atgcgcttgg caccatcaac aataaatccg
     1321 ataaattgac cggcatcttc atccaaacta ttgcgataac gtttgtctaa aatttgaatg
     1381 aaactggtaa tggtacgcaa aggctcttgt aagtcgtgag acgctacata agcaaaatgc
     1441 tctaattcct gattggtatt ggtaagttca atattctttt gctcgagcgc ttcttcagtg
     1501 cgcttttgag tttcaatgtt ttctatgata gcaacagata aactaatgct gcctatgctg
     1561 aacatattcg acatcacctc ccgtgcccag aatgaagtgc cgtccttgcg gtcaaagcgc
     1621 ttttctatgg agtaatgcgg gatttcattt ttatatattt tttggagcca gttacctgag
     1681 ctaatggcat ctgcaggatg cactaagtcg ataattggaa gattaaataa ttccgtatca
     1741 ctataaccca tcattttggt aaatgccgga ttggcatcta taataacacc gggtgctgcc
     1801 gtaattacaa tacccattgg ggagttctca tatatggcgc ggaatttcac ctcgctttct
     1861 atcaatgcct gctcactgcg tttctgacta tcgatatttt ccagtaatga taataatagt
     1921 gtttcgccat gtgcacctac cattgtactg caggtcattt tcgaccaagc tacaacgccg
     1981 ttttttgtaa ttaatcgctt ttcaacgaat gcattttcaa tctgaccatt gcgcaatgct
     2041 tccatatacc gcactactcc cggcttatcc tcttcatgaa ataattcaca gaattgtgta
     2101 tttctgatct catcccatgt ataaccatgc ctttgcatga aggtgtaatt cgattcaaca
     2161 atatttcctg caaaatcagt gagcactatc cctaaagcgg cgttgttaaa tacagtttta
     2221 aatcgctcct ctccggcttt caccaatgct tgtaaattcc gacggtcgtc aagatcacgc
     2281 actactgaaa tgcgtacttt ctgattgtga tgttgaattg tttttccggt cacctcaacg
     2341 tttcttgctt caccgctatc tatgcgaatg atgcgggaag tatataattc gtcggagttg
     2401 gtttctacaa tgcgtccaac cttttcttta aactcggaat caacaaaatc aagaaaattt
     2461 ctgccaacaa catcctccgg cgagctgcat ccgaaaatgc gcataaaagc attattcacc
     2521 atctgatatt tgccgtcaac aataatactc atgccatcca acgcatgatc aaagaaaaac
     2581 tgaaggcgcg attgcgtatc ctccattctt tgctcctgtt gcatgatatt ggtgatgtcg
     2641 cgaccaatag cataaatatg attggtgatt ttatttaaaa tggcatcata cgaaatagtt
     2701 ttaatagtgc catttttcat acgcttgcgc aaagtgaaat ttagcacctc acttctatcc
     2761 tttgaaaatg tactcaacaa atcatacaat ttcggaagat catcttggag tataagatcc
     2821 cagccgccat tgagtgcaaa caattcctct ctggtatatc ctgaaatgcg ctcgtaagca
     2881 ttattgaaat caattacttt cccgtcaata tccaatacca tcatcacatc agcggatatg
     2941 cggaaaaata attcgaagtc ggtaggaaat cgaacagctt tggtgttatg tgtttgctgt
     3001 tttggcaatg caggataaaa ttgctcccta aaaatacatg aaattcgccg aatgcttact
     3061 cgccaatggt caaaggcgct tgtacattac ccccgaactt cctttgaata aatggattga
     3121 taaatacaac aagcaggatc aggagggtgc cgatgtaaaa tccgggttcg aggtgatcat
     3181 tttcatgaaa gaaaactatc gccataataa taccgtaaac aggttccaga tttaaggtga
     3241 tagacgccgt aaatactgaa atatgacgca tgctgtatag ggaaagagta aatggcacta
     3301 ccgtgcaaat accggcaaat aaaatcagat tgatgccatc catggtcgat ggttgtaaat
     3361 ctgcttccgg ttgccaatgc aggtaaagcg gcataatggc gctcagcaaa atgaaacctg
     3421 tgaatatttc gatgaacgta atgtttaatg gtgcagtaac cttcgtcatt ttcttgttca
     3481 gcaaggaaaa caaggttgca aatacagtgc ttattaaccc cagaataatc cccgttttgt
     3541 aatttccgct gaaatgaaaa atacaataca tggccggtat caccagcaat gcaagcaata
     3601 attcatgcca cacaaatttt ttgccgaata acagcggttc gaaaacagat acaaaaaagg
     3661 atgtggttgc caaacaagtc agtgcgacag aaatattcga cgatttaata gatccgtaaa
     3721 atgatatcca gtgaatggta actaacacgc cgataccaat taatcgcagc agcgtttttt
     3781 tatctacttg ccgaatagtt ttccaaagtg aaggaataca aagtaatatg gctgaagtga
     3841 tacccattcg gtaccacacc aatattgttt catttaaatc aataatgcga ccgaacaccg
     3901 ccgtaaaacc ccacaagaaa acacagagat gataggccca ataggccttg cggtatgccg
     3961 gatccatagc acaaagttag agaattccgt tgtcggcaaa actgtaataa tcacggtcgc
     4021 cgatgatcaa atggtcgaga acggcaatct ccatcagttt ggcggcatcg cgtagctttt
     4081 tggtaacgga aatatcggca tcgctgggtt gaagatttcc gcttgggtgg ttatgtgcca
     4141 gaatgatgcc tgatgccagt ttctgcaatg ccaaacgcaa aatgattttc acgtccacaa
     4201 cagttcctgc cacccctccg gagcttactt tttctttgct gatgatgcga tttgccttgt
     4261 tgaggtagat cacccaaaat tcttcgtgag ccaggtcgct taaatgagga tgaatgtttt
     4321 cgaaaatatc gttgctcgat cgaacctgcg tttttagctc ggcaacagca agtgcatggc
     4381 gtcgaccgag ctccattgcg gcaattatgc ttacggcttt cgcttcgcca attccgttga
     4441 attgcgtcaa gtcgcttacc tgcaatttgc cgatggcatt cagatcgttg ccataatgag
     4501 ctaaaatgcg tgtcgatagc tcaacagccg tttctccggt attcccggaa ccgataagaa
     4561 tagcaatcaa ttcggcaacg gtaaggcttt ggcgaccgaa agcgagcatc ttttcgcgag
     4621 gacgatcctc ctcagccaga tgcttaaggg tataattcgt tgaagtttcc atcacagttc
     4681 atacatcaca tccctcgctc caaaaataaa aaaacccggc ttcatggatg aaaccgggca
     4741 aaaaaaaata gtgcttgaat taattatttc gcaactgcct tcttcattaa tgaagatttc
     4801 aggttggcag ctttattctt gtgaatcaca ttgcgtttcg ccagcttatc aatcatagac
     4861 accacttcag gcaacatttt gtcagcttcc ttagaatctg tagttgcacg gagtttacgg
     4921 atggcattac gtgttgtttt gccatagtaa cgattcgctt cattgcgctt ttccgactgg
     4981 cggattgact tcttagcggc cttatgatgt gccattgttt ttcctcttag tttttaaaat
     5041 ggagtgcaaa tatagggagc attgtggata attacaaata gaatgttggg ttttacttac
     5101 cacaaacgga tttttaacgc cataacgcca attcgagccc tccttacttg tcgaaatgac
     5161 ctccaaacac ggctgagagc ctctaaacgg ccttaaatgg caattgcagg accaaaattc
     5221 tccccacccg ggacttctgt caggctatca tagccaaaaa agcctcttca tcaatgatct
     5281 ctacagtccc taaagcctta gccttgtcca gcttagaccc tgcgctttcg cctgctacca
     5341 ggtagttcag gttcttggag acagagccta tcagtttgcc gccattggct tctaccagtt
     5401 ctttggcctc gtcgcgggta aagcgggtca gggagccggt gaacaggaag gtcttgcctg
     5461 aaagcttgcc actgcctcct gcggttttct cgttcgccat gttcaacccg tcttctttga
     5521 gcttcaaaat catctgttgg ttgaaggcat tcgaaaaata ttgatgaatg ctcgccgcca
     5581 cctttggacc aatgccttca attgcctggt atcgttccag gtcccagtca aacaactctg
     5641 tcagatcgct gatattcttt accaggtctt ttgccgtttg tgtgccgagg tgacgaatgc
     5701 caagcccgtt caccaatcgc cacaacggtt gctttttcga agcctcaata ccatcgatga
     5761 gtttggcgat acttttttct ttccaccctt cgcgacctgc aagatcggat gtcttcaaat
     5821 caaaaatatc gggtatggtg gtaatctttc ctgcctgata taattcgctg atggtttctc
     5881 gaccgcagcc atcgatatcc atggcatcct tgctcacgaa atggatgagg cgttcttcca
     5941 tttgtgcagg acattcggga ttaatgcatc ggtaaacagc ttcttcttca ggtttgtaaa
     6001 tagcttggtt gcacgatgga catt
//
LOCUS       DAOQOU010000183         5945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_7909_length_5945_cov_5.990832, whole genome shotgun sequence.
ACCESSION   DAOQOU010000183 DAOQOU010000000
VERSION     DAOQOU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7909_length_5945_cov_5.990832"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PLM90_12575"
     CDS             <1..784
                     /locus_tag="PLM90_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90263.1"
                     /translation="RLAEACAEFGLGMGLGSTRRLLDDNTWFEDFNLRPVIGPELPFF
                     ANLGIAQVESLLAERKTDKIHALIARLQADGLIIHINPLQEWFQPEGDRFKRSPLETI
                     QEFIESADYQVIVKEVGQGMGPNSIKALLQLPIDALDFGAFGGTNFSKLELLRNRSGD
                     ASLYEDLAYIGHTAAEMTGFVSEAVWELGEKVQCKQVIVSGGIGNFLDGYWCMEKLPL
                     TGIYAQGSAFLKFAQLDSRALKNYIVSQREGLILCKQFLRVR"
     gene            833..2173
                     /locus_tag="PLM90_12580"
     CDS             833..2173
                     /locus_tag="PLM90_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HMZ90264.1"
                     /translation="MQKIKGFSKLSKTDKIAWLAKHFLTSNPLDIAREFSSYMHSNAE
                     MQKLFDGISENTLTNFYLPYGVAPNFVINGRTYVVPMVIEESSVVAAAAAGAKFWSDR
                     GGFNAEVISTTKVGHVHFLWRGDSEKLFHFFDTELKEALLAEVKHLTANMESRGGGLR
                     TLELIDCTELEPEYFQLKATFETMDAMGANFINSVLEAFAECLTEEVKHYKDFSGAEK
                     NAEVVMSILSNYTPECLVKAWVECKVSDLGGFPEANLTAQQFARKFSTAIRIAKHDVY
                     RATTHNKGIFNGIDAVVLATGNDFRAIEACGHAYASKNGQYGSLSDCEIVGDTFRFSI
                     TLPLAVGTVGGLTKLHPLAKRSLEMLGNPSAKELMMIIATVGLAQNFSAVKALVTSGI
                     QKGHMKMHLTNILRHFGATDTEIQLATTYFADKTVSFSSVRDFLNNSRNLATEK"
     gene            2223..3167
                     /locus_tag="PLM90_12585"
     CDS             2223..3167
                     /locus_tag="PLM90_12585"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HMZ90265.1"
                     /translation="MQRDFYAHGKLLISGEYLVLKGALALAVPTVYGQHLSATIEQKN
                     ADVINWTSFDNNGNKWFNATLDAKSLQVKGPLTGDKNIADTLRNIIIAIRKQQPQYLL
                     QGDINISTKLEFPNDWGLGSSATLLTNLCKHANADAMIVNAEVFKGSGYDIATAQANA
                     PILFRIDDEGPVFDSVNFFPPQHEQFRFVHLGEKQNSRIAVEQFLDETKDKKLFNEIE
                     TISEISEALLFCDDFSDFMQLLDDHEEMMQYVLQEEKIKTARFNDFPGVVKSLGAWGG
                     DFVLAAADMDGADIDTYFKEKGLHTVISYADMVLSAHA"
     gene            3195..4229
                     /gene="mvaD"
                     /locus_tag="PLM90_12590"
     CDS             3195..4229
                     /gene="mvaD"
                     /locus_tag="PLM90_12590"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HMZ90266.1"
                     /translation="MQINWKSPSNIALIKYWGKHGVQLPNNPSLSLTLREANTTTSLD
                     LKKKRSKKNIEIDFLFEGERNDKFAAKLETFLASVTQELPWLTKNRLVLRSENSFPHS
                     AGIASSASSMSALALCLMSTNRMLTKTPEDDATFLRMASRLARLGSGSAARSVYPGFT
                     VWGKTTSVKNSSDDHAIIAPANYHADFNELQDSILIVHAGEKSVSSRAGHALMNDHPM
                     AAQRYKNAHARLKQLLDALKHADWQLFCKVVETEALELHALMMTSDPSFILMHPNTLA
                     IIERIRFFRATTNIPVCFTLDAGPNVHILYPYFAKKEVRNFIQSELLQFCVKKKVIYD
                     QMGDGPSMLL"
     gene            4229..5053
                     /locus_tag="PLM90_12595"
     CDS             4229..5053
                     /locus_tag="PLM90_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90267.1"
                     /translation="MIEMQYPSKLMLFGEYAVIAGGEGLAIPWPDFFAEWVEQPETAS
                     AYRPHLERFAKWLHEQSFDSILDTDTFLQELEDGLDMHSNIPIGYGVGSSGAVVAAVY
                     DRYAKEKLPISDLIALRNTLASMENFFHATSSGLDPLVCYVQQPIHITPYAINVVSTP
                     ISFGNLQMRLLDCGFARATHPLVSAFKEKLSDPHFHTALQEYIQLSNACIEHVLSWHE
                     TKFEQALRKLSAWQFHNFDFAIPEHLRKEWQDAVLSGNCFYKLCGAGGGGFMLVFS"
     gene            complement(5060..>5945)
                     /locus_tag="PLM90_12600"
     CDS             complement(5060..>5945)
                     /locus_tag="PLM90_12600"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90268.1"
                     /translation="IFLPDAERQWNTEKYNKKLSQMARVMAEGPGSLPALIGMCEVEN
                     ASVLNDLIKEPVLANAKYKYVHFDSPDERGIDVALLYNPEYFKPTTSTAIPVKLVHGD
                     STDHTRDILLVSGTIMQNGKQETLHVFVNHWPSRREGEEASAWKRATAAAVLKAAVDS
                     LNRNYTDPNIIIMGDFNDTPFDASIQKVLGAQQEAATYNDESLVDLMAAKQAGGLGSY
                     NYKGNWQALDQIIVSETMLDGTGLQIDGDKTTFIRKDWMLFHHNTYGDSPNRTYSGTK
                     WYGGFSDHLPAYTALLIK"
BASE COUNT         1708 a         1286 c         1318 g         1633 t
ORIGIN      
        1 gcgattggct gaggcttgtg cggaattcgg actcggtatg ggactcggct ccacaagacg
       61 ccttctcgac gacaacactt ggtttgagga ttttaacctg aggccggtga taggtcctga
      121 actgcccttt ttcgcaaatc tgggcatagc acaagtggaa agccttctgg cggagcgaaa
      181 aacagataag atacatgccc taattgcccg tttacaggct gatggcttga ttatacatat
      241 caatcccctc caggagtggt tccaaccgga gggcgacaga tttaagcgct cgccgcttga
      301 aacgatacaa gaatttattg aaagtgctga ttatcaggtg attgtgaaag aggtgggtca
      361 gggaatgggg cccaatagta tcaaggcact cttgcagctc cctatcgatg cactggattt
      421 cggggctttt gggggtacaa acttttctaa gcttgaattg ttaagaaaca gatcagggga
      481 tgcatccctt tacgaagatc tggcgtatat aggtcatacg gccgctgaaa tgacgggatt
      541 tgtaagtgag gctgtatggg aactgggcga aaaggtgcag tgtaagcagg tcatcgtgag
      601 tggaggtatc ggcaatttcc tggacggata ctggtgtatg gagaaattac ccctcaccgg
      661 catctatgcc caaggatcgg ctttcctgaa attcgcacag ctggacagcc gggcgttgaa
      721 aaactatatc gttagtcagc gggaagggtt aattttgtgt aagcaattcc ttcgggtgcg
      781 ataatttttt cacgcagagc cgcagagttg caggggttct aaatttggaa taatgcaaaa
      841 gatcaaagga ttttcgaaac tgagtaaaac agataagatc gcgtggttag cgaaacactt
      901 tctgaccagc aatcctttgg atattgcgcg cgaattttcg agctatatgc actcaaatgc
      961 cgagatgcag aaactgttcg acggtatttc tgaaaatacg cttaccaatt tctatctccc
     1021 ttatggcgtt gccccgaatt tcgtgatcaa tggtcgcacg tatgtggtgc cgatggtgat
     1081 tgaagaatct tctgtggtgg ccgcagccgc cgccggtgca aaattctgga gcgatcgcgg
     1141 tggatttaat gctgaagtca tttccaccac taaagtaggc cacgtgcatt ttttatggcg
     1201 cggcgatagt gaaaaactat tccacttttt cgatacagaa ctcaaagaag ctttattggc
     1261 agaagtgaaa cacctcactg caaatatgga atcgcgcggt ggtggtttgc gtacgctcga
     1321 attaatagat tgcacggagc tggaacctga atattttcag ctgaaagcaa ctttcgaaac
     1381 catggatgcc atgggtgcga attttatcaa ttctgttctc gaagcatttg ccgaatgtct
     1441 gaccgaagaa gtaaagcatt ataaagattt ttccggcgca gaaaaaaatg cagaagtagt
     1501 gatgagcatt ctcagtaact acactccgga atgtctcgta aaagcctggg ttgaatgtaa
     1561 agtaagcgat ctcggcggat tcccggaagc taacctcaca gcacagcaat ttgcacgtaa
     1621 attcagtacc gccatccgca tcgccaaaca cgatgtatat cgcgctacaa cacataacaa
     1681 aggaattttt aatggcattg atgcagtagt tcttgctacc ggaaatgatt tccgcgccat
     1741 cgaagcctgc ggtcatgctt atgcctctaa aaatggtcag tacggcagtc tctctgattg
     1801 cgagatcgtc ggtgatactt tccgtttttc catcacccta ccgcttgcag tgggcactgt
     1861 tggcggactc acaaaattac atccgctcgc taaacgctca ctcgaaatgc tgggcaatcc
     1921 tagtgccaag gaattaatga tgatcatcgc aacggttggt cttgcacaaa atttctccgc
     1981 tgtaaaagca ctcgttactt ccggcattca gaaagggcac atgaaaatgc acctcacgaa
     2041 tattcttcgt catttcggcg caacggatac cgaaattcag ctggctacaa cctatttcgc
     2101 cgataaaaca gtatccttca gcagtgtgcg cgattttctg aataacagtc gcaacctggc
     2161 tacagaaaaa taatgtcgca cagtaagtaa agcgcattcc ttcacgtatc tttactaccg
     2221 tcatgcaaag agatttttac gctcatggta aattattgat cagcggcgaa tacttagtat
     2281 taaaaggcgc actcgctctt gccgtaccta ctgtttatgg tcagcatcta tctgcaacca
     2341 tcgaacaaaa aaatgccgat gttatcaatt ggacaagctt cgataacaat ggtaataaat
     2401 ggttcaatgc aacactggat gctaaatcat tacaagtaaa aggcccattg accggcgaca
     2461 aaaatattgc cgacacttta cgcaacatca tcattgccat tcgcaagcaa caaccacaat
     2521 atttattgca gggcgatatt aatatcagca cgaaactcga atttccaaat gattggggat
     2581 tgggcagcag cgccactttg ctcactaatc tatgcaagca tgccaacgca gatgccatga
     2641 ttgtgaacgc agaagtgttc aaaggcagtg gttatgacat cgccacggca caagctaatg
     2701 caccgatttt atttcggata gatgatgaag gtccggtttt cgattcggta aatttcttcc
     2761 caccacaaca tgagcaattc cgatttgtgc acctcggaga aaaacaaaat tcacgtattg
     2821 cagttgaaca atttctggat gaaacaaaag ataaaaaatt gttcaatgaa atagaaacca
     2881 tctcagaaat cagtgaagca cttttatttt gtgatgattt ttcagatttc atgcagctcc
     2941 tcgatgatca tgaagagatg atgcaatatg tgttgcagga agaaaaaatt aaaacagcac
     3001 gattcaatga tttccccggt gtagtaaaat ctcttggcgc ctggggtggc gattttgtgc
     3061 tggctgccgc cgatatggat ggtgcggaca tcgataccta tttcaaggaa aagggattgc
     3121 acaccgttat ttcctatgcg gatatggtgc tgtctgcgca tgcataaata atgattccta
     3181 aacaattcta cacaatgcag attaactgga aaagcccgtc aaacattgcc ctcatcaaat
     3241 attgggggaa gcatggtgta cagttgccca ataatccaag cttatcactc actcttcgag
     3301 aggcaaatac caccacgtcg cttgatttga aaaaaaagcg gtcgaaaaaa aatattgaaa
     3361 ttgatttctt attcgaaggt gaacgcaatg ataaatttgc agccaagctg gaaacatttc
     3421 ttgcctctgt aacacaggaa ttaccatggc taacaaaaaa cagattggta ttacgcagtg
     3481 aaaatagctt tccgcattcg gcaggtatcg caagctcagc atcatctatg agcgcattgg
     3541 cattatgcct gatgtcgaca aatcgcatgc tcacaaaaac acctgaagac gacgctactt
     3601 ttttgcgcat ggcaagtcgc ctcgccagat taggtagtgg ttctgcagcg cgcagtgtgt
     3661 atcccggatt tacggtttgg ggaaaaacca cttcggtaaa aaacagcagc gacgatcacg
     3721 ccattatcgc acctgcaaac tatcatgccg attttaatga attacaagat agtatactca
     3781 ttgtgcacgc cggagaaaaa tcggtgagca gtcgcgccgg ccatgcactc atgaacgatc
     3841 atccgatggc ggcacaacgt tataaaaatg cccatgctag attaaagcaa ttattggatg
     3901 cattgaagca tgccgactgg caattatttt gcaaagtagt tgaaacggaa gccttagaat
     3961 tacatgcgct catgatgacg agtgatccat catttatttt gatgcatcca aatacgttgg
     4021 ccattattga acgtatcaga tttttcaggg caacaaccaa tatacctgtt tgctttaccc
     4081 ttgatgcagg acctaacgta cacatcctgt atccatattt cgctaaaaaa gaagtgcgta
     4141 attttattca atcagaatta ctgcaattct gtgtgaagaa aaaagtgatc tatgatcaaa
     4201 tgggcgatgg cccttcaatg ttattgtgat gatagaaatg caatatcctt ccaagctgat
     4261 gttattcggt gaatacgctg taatagcagg tggagaagga ttggctattc cctggcccga
     4321 tttttttgcc gaatgggtgg aacaacctga aacagcttca gcatatcgtc cgcatctcga
     4381 acgatttgcc aaatggctgc atgaacaaag ctttgatagc atattggata ccgatacttt
     4441 tttacaagaa ttggaagacg gcctcgacat gcactcgaat attcctatcg gctatggagt
     4501 tggcagttca ggtgcagttg ttgctgctgt ttatgatcgc tatgccaaag aaaaattacc
     4561 gatatctgat ttgatagcac tgcgcaatac acttgcatca atggagaact tttttcacgc
     4621 aacaagcagc ggtcttgatc cattggtatg ttatgtgcaa caacccattc acattacgcc
     4681 ttatgctatt aatgtggtaa gcacaccaat ttcttttggc aatttacaaa tgcgattgct
     4741 cgactgcgga tttgcacgtg ctacacatcc gttggtatct gcatttaaag agaaactgag
     4801 cgatcctcat tttcataccg cactgcagga atatatccaa ttatcaaatg catgtattga
     4861 gcatgtattg agttggcatg aaacaaaatt tgaacaagca ttgcgcaaat tatcggcatg
     4921 gcaatttcat aattttgatt ttgcaatacc ggagcacctt cgcaaggaat ggcaggatgc
     4981 agtattatcc ggaaactgct tctacaaatt atgcggagca ggcggaggtg gatttatgct
     5041 ggtattttca taacacgcat cactttataa gtagtgcagt atacgccggc aggtggtctg
     5101 aaaaaccgcc ataccatttt gtgccgctgt aagttctatt cggagaatca ccgtaggtat
     5161 tatggtgaaa caacatccag tcttttctga taaatgttgt tttgtcgcca tcaatttgca
     5221 aacctgttcc atcgagcatg gtttcggaaa ctattatctg gtctaatgcc tgccagtttc
     5281 ctttgtagtt atagctcccc aatccacctg cctgttttgc agccatcagg tctacaagcg
     5341 attcatcatt ataggttgca gcctcctgtt gagcacctaa cactttttgt atggaagcat
     5401 caaagggcgt gtcattaaaa tcgcccatga taataatatt cggatcggta taattgcgat
     5461 tgagactatc aacggctgct ttgagtactg ctgcggctgt agcgcgcttc catgcactcg
     5521 cttcttcacc ttcgcggcga ctaggccaat gatttacaaa tacatgcaat gtttcctgtt
     5581 tcccattttg cataattgtt cccgaaacaa gtaatatatc gcgtgtatga tctgtggaat
     5641 caccatgtac caattttacc ggtattgctg ttgatgtagt aggcttgaaa tattccggat
     5701 tataaagtaa tgccacgtca attccgcgtt catcaggcga atcgaaatgg acatatttat
     5761 attttgcatt tgccaacaca ggctccttaa tcaagtcgtt taatacagaa gcattttcca
     5821 cctcacacat acctatcaat gccggcaatg agccggggcc ttcagccatt acacgagcca
     5881 tctgcgaaag cttcttattg tatttctcgg tattccactg gcgttcagca tcaggtaaaa
     5941 aaata
//
LOCUS       DAOQOU010000184         5903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8015_length_5903_cov_5.719733, whole genome shotgun sequence.
ACCESSION   DAOQOU010000184 DAOQOU010000000
VERSION     DAOQOU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8015_length_5903_cov_5.719733"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..841)
                     /locus_tag="PLM90_12605"
     CDS             complement(2..841)
                     /locus_tag="PLM90_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ90269.1"
                     /translation="MLLFINTIQDYFSTIPDLHRTLIIWGGLVFFYLIEYAIPLIRFQ
                     YKKIQHAGVNIFFTITTILVNVSLAFILLKASDYVSDHSIGLLYIFSLSPWVFFIAGL
                     LLMDLIGAYTVHYVEHKVKWMWKFHLIHHADTYVDTTTANRHHPGESIFRFVFTTMAV
                     IITGAPMWIVMVYQSLSVVLSQFNHANIKLPDWLDKGLSWLIVSPNMHKVHHHYMQPY
                     TDSNYGNIFSFWDRVFGTFKHLQPKQIVYGIDTHMGVEENSKIGNLLQIPFQPYRKPG
                     GKG"
     gene            complement(907..2802)
                     /locus_tag="PLM90_12610"
     CDS             complement(907..2802)
                     /locus_tag="PLM90_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HMZ90270.1"
                     /translation="MSYKSLQDFVDALERAGELVRVKEFVDPKLEITEIADRYIKSDK
                     PALLFENTGTGFPLLINSMGSYKRMCMALGVDHLDAIGDDIAGLFKKFTSPKQGLLDK
                     VMMLPELGKVASWMPKELKGKGACQEVIINDPDLTKLPVMTCWPADGGPFITLPVIHT
                     VDPENGIRNVGMYRMQVFGPKLTGMHWHKHKVSAGHFNKYKQLGKRMPVSVVLGGDPV
                     YTYCATAPLPPNVDEYLLAGFIRKRKVELVKCITNDLYVPADADFVLEGYVDPEEDFI
                     YEGPFGDHTGYYSLADYYPKFHITCITHKKNAIYPSTIVGIPPQEDAWIGKATERIFL
                     TPIKLTMLPEVEDMELPIEGVFHNLTIVKIKKEYAGHAQKVMNAMWGAGQMMFNKILV
                     ICDTSVDIHNYDAVAKAISEHVDPAQDIYFSTGPMDVLDHSCSKFAFGGKMCVDATAK
                     LPEEQTAAHGKKEYPAVDGNVLMQKYPEITHVNDSLLSLGISFLTIALKKSKQGHVRQ
                     LHTILSAEPGLQHVKFIAYVDHTTEASDNGVVTWNTSNNMDARRDVFITAGTSGNSIC
                     GMDGTRKTKQFDNFDRPWPNVIVMDDKTISTIDEKWAQLGLGPFIVSPSHKYRRQIVG
                     RGAVVEE"
     gene            complement(3099..4658)
                     /locus_tag="PLM90_12615"
     CDS             complement(3099..4658)
                     /locus_tag="PLM90_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90271.1"
                     /translation="MKRICIASLLLFASLAAIGQTSFSRLYWKYPYVGFGVAVNYDEN
                     YIYIVGRGNSYWDGFLAANRFFIKSDYFGDPISTSYIFRDSIGVWYGGYTSGLEFISD
                     SILVCAGYFSRYDTLFHPWNSQHLGVLYKYNISGDTVLTKKYFGDGYTEFERIVRNDG
                     EITLGGYTSDTTFDGVYGYLIRVEENGLVLNETKCGDGIYDEISGLMDFNGNDRLLAG
                     GVIDETGSDLISINNGIIHKIDNLGNLYFSKEIGTPGNDSEVEVKVAKNGQSYIVRQY
                     IDTVINEGDYEYVQYIGKTDTNVNFIWRTFLNDEFYKYIYTLRTFEDGSIVAVGVTIV
                     DNTYEPHGYIVKLDSNGTVLWERDHTNNPAGNHYLYDFQKMPDGGYICSGMGADTVEG
                     GGIESMCWLLRLDSMGCLIPGCDEVSVPDIAVNSETYLSIYPNPFGDAATVEVHLPQD
                     FNINGNQSLRFDIYDMQGRLIDNYSNIYPSNPGETIRFKMYRNKMTVGNYLATLKYGD
                     EVLNTIQFVIN"
     gene            complement(4663..>5903)
                     /locus_tag="PLM90_12620"
     CDS             complement(4663..>5903)
                     /locus_tag="PLM90_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90272.1"
                     /translation="NGDTDDGLEIKCNTFEEGKYDISLISGATTATMKKNQGACGTLT
                     SPANNLFTDPSFADINTQIKTSSDITTTNYYKFSFHEDDDAGGSPFAAPAIYSNTIDP
                     LPDGDVFESGCNDVETFTTTNLDDYCSTNFPSGSGGSGRMSAPKATKRRPPTLMMPLT
                     YNPYEYLKPAGVDYEGFLKNIRFNMEASYYLQNELIDSLNILLNEYESPLATSIKVQL
                     LEDLSDYDNAEVLSAELTESPDAELNGYNSSLLSLGLSLSRDTLTWLQTTEEQFSSIK
                     TEAQRNDREGIQAKLLMELIAGGAYIEPINELNEELTTEELRLLQQSMPQSKPNSIQV
                     YPHPVGSGSVVEINLKSIQSNARFIITDVQGRNVLQFPLDNLVSYVKLDNTLLRPGVY
                     IGYVAGATGEQISKQIVVIQ"
BASE COUNT         1751 a         1288 c         1069 g         1795 t
ORIGIN      
        1 ctcacccctt cccacccggc ttgcgatatg gctggaacgg gatctgcaga agattaccta
       61 ttttactatt ctcctcaaca cccatatgtg tatctatacc atacacgatc tgcttaggct
      121 gaagatgttt gaaggtaccg aaaacacgat cccaaaaaga aaatatattc ccataattag
      181 aatccgtata cggctgcata taatgatgat gcaccttatg catattcggt gacacaatca
      241 accaactcaa acctttatcc agccagtcag gcaatttgat attcgcatga ttgaattgcg
      301 ataagactac cgataatgac tgatacacca tcacgatcca cattggtgca ccagtgatga
      361 taacagccat ggtggtaaat acaaatcgaa aaatactctc ccccggatgg tggcgattag
      421 ccgttgttgt atcaacatac gtgtcggcat ggtggatgag atgaaatttc cacatccatt
      481 ttactttatg ctccacataa tgcaccgtgt aagcaccaat aagatccatc agtaataatc
      541 cggcgataaa aaacacccat ggcgataatg aaaatatgta aagtaatcct atcgaatgat
      601 cagaaacata atctgaagcc tttaataaaa taaatgccaa agaaacattt acaagtatag
      661 tagtaatcgt aaaaaaaata tttacacctg cgtgttgtat ctttttatac tgaaatcgaa
      721 tcagtggaat agcatactcg ataagataaa aaaacacaag tccgccccaa atgatcaatg
      781 tcctgtgcag atcaggaatg gtgctgaaat aatcttgtat ggtgttgatg aaaagtaaca
      841 cgaatggaaa ggatttaacg ggttaaaacg gattgtttcc tctaaggtaa aaaatccatc
      901 caaactttac tcctccacta ccgctcccct gccaacgatt tgtcgtcgat acttatgtga
      961 cggcgataca atgaaaggcc ctaagccaag ttgtgcccat ttctcatcta tcgtgctgat
     1021 ggtcttgtca tccataacga tcacattcgg ccatggtcgg tcgaaattat cgaactgctt
     1081 tgttttgcgc gtaccgtcca taccacaaat actattgccc gatgtgcctg cagtgataaa
     1141 tacatctctg cgtgcatcca tgttgtttga agtattccag gttaccacac cattgtcgga
     1201 ggcctctgtt gtatgatcta cgtaggcaat aaatttaaca tgctgcaaac ccggttcagc
     1261 tgataatatg gtatgcaatt gacgcacatg cccttgtttt gattttttta gtgcaatggt
     1321 caaaaaggaa atacccaatg acaataaact atcattcaca tgcgtaatct ccggatattt
     1381 ttgcataagc acgttcccat caacagctgg atattctttt ttaccatgtg cggctgtttg
     1441 ttcctcaggc aattttgctg ttgcatccac acacatctta cccccaaaag cgaatttgct
     1501 gcaggagtga tcgagcacat ccattggtcc tgtgctgaaa taaatatcct gtgcaggatc
     1561 tacatgctcg ctgatggctt ttgctacagc atcgtagtta tgtatatcga cagaagtatc
     1621 gcaaatcacc agaattttat taaacatcat ttgtcccgca ccccacatcg cattcatcac
     1681 tttttgtgcg tgacctgcat attctttttt aatttttact attgtaagat tgtgaaaaac
     1741 accttcaatt ggcaattcca tatcttccac ttccggaagc atggtgagtt tgatcggcgt
     1801 taaaaatatt cgctccgttg cttttccaat ccatgcatct tcctgtggcg gaatgcctac
     1861 aatggtagat ggatatattg catttttttt atgcgtgata caagtaatat gaaatttcgg
     1921 ataataatct gccaatgaat aatatccggt atgatcacca aaaggacctt cataaataaa
     1981 atcctcttcc ggatctacat atccttccaa cacaaaatcg gcatcagcag gtacatacaa
     2041 atcatttgtg atgcatttta ccaattctac tttgcgcttg cggataaatc ctgccaataa
     2101 atattcatcc acgtttggtg gcagcggagc tgttgcacaa tatgtataaa caggatcacc
     2161 gcccaatacc acacttacag gcatgcgttt gcccagttgt ttatatttat tgaaatggcc
     2221 tgcacttacc ttgtgtttat gccaatgcat tccagtaagt ttcggaccaa acacttgcat
     2281 gcggtacata cctacattgc gtattccgtt ttccggatca acagtatgaa taacaggcaa
     2341 tgtgatgaag ggccccccat cagcaggcca gcaggtcatt accgggagtt tcgtaagatc
     2401 aggatcatta ataatcactt cctgacaagc acctttccct tttaattctt tgggcatcca
     2461 ggaggctact ttacctagtt cgggaagcat cattacttta tccagcaatc cctgctttgg
     2521 cgaagtaaat ttcttaaaca gacccgcaat atcatcacca atagcatcca aatggtcaac
     2581 tcccaaagcc atgcacatgc gtttataaga gcccatgcta ttaatcagca ggggaaaacc
     2641 cgtccctgta ttttcgaaca gcagcgcagg cttatctgac ttgatatacc tgtcggctat
     2701 ctccgtgatt tccagtttag ggtcgacaaa ctccttcaca cgaaccagct cccctgccct
     2761 ttccagtgca tccacaaaat cctgtaaact cttgtagctc atagcgcaaa ggtagtaaca
     2821 gggagggaga aaaagtgttc agggggtgag tgggtcagtg ggtgagtggg tgagtggtga
     2881 gctgaagtag gtgtttcgta ttgagtttgg aggttgtgaa acactcgaca catgcacgaa
     2941 tcacaattta gtagtgagta gtgggtagtg agataattga tgattaggat tgatgttgga
     3001 tattgaagat ttgaagggag gaatattaag gatttgatgg attgaaggtt tgaaggcgcg
     3061 gggaaaattg cgggaatatg aaaataatgg aattatggtt aattaattac aaattgaatt
     3121 gtgtttaaaa cttcatcccc atactttagc gttgcgaggt aatttccaac tgtcatttta
     3181 ttgcgataca ttttaaaacg tatggtttct ccggggttgc ttggataaat attactgtaa
     3241 ttatctatca gtctgccttg catatcataa atatcaaatc tcagtgactg gttgccgttt
     3301 atattaaaat cctgaggcaa atgaacttct actgttgctg catctccaaa cgggtttggg
     3361 tatatactta gataggtctc agaattaaca gctatatcag gtaccgacac ctcatcacaa
     3421 cccggaatga ggcaacccat gctgtctaaa cgtaaaagcc agcacataga ttctattcct
     3481 cctccttcaa ctgtatctgc ccccatgccg gagcatatgt atccgccgtc aggcatcttt
     3541 tgaaaatcat atagataatg gttaccggca ggattatttg tatggtcacg ctcccataat
     3601 acggttccgt tactgtctag cttcactatg tatccgtgag gctcgtaggt attatctaca
     3661 atggtaacgc cgactgctac gatactgcca tcttcaaaag tgcggagagt ataaatgtat
     3721 ttgtaaaact catcattcag gaatgttcgc cagatgaaat ttacatttgt gtctgtttta
     3781 cctatatact gtacatattc ataatcccct tcgttaatca ctgtatctat atactgacgc
     3841 acaatataag attgcccatt ctttgcaacc ttaacctcaa cttcagagtc atttcccggc
     3901 gtgccgattt ctttggagaa atataaattc cctaaattat caattttgtg tattatcccg
     3961 ttgttaattg aaattaaatc agatcctgtt tcatcgatta caccacctgc caataacctg
     4021 tcattcccat tgaaatccat caatccacta atttcatcat aaatcccatc accgcattta
     4081 gtttcattta aaacaagacc attttcctcc actctgatta agtaaccgta taccccatca
     4141 aaagtagtgt cgctggtata accgcccaaa gtaatttcac catcatttct tactatcctt
     4201 tcaaattctg tataaccatc gccaaaatat tttttagtta gaactgtatc acctgatata
     4261 ttatatttat aaagaactcc taggtgttga ctattccaag gatggaacaa tgtgtcatac
     4321 cttgaaaaat aaccagcaca aactaaaata ctatcagata taaactccaa accactggta
     4381 taaccaccat accaaacccc aatgctgtct cgaaatatat aactggttga aatcgggtca
     4441 ccaaaatagt cagattttat aaaaaaccga tttgcagcaa gaaatccatc ccaatagcta
     4501 tttccacgac caacaatata aatatagttc tcatcataat ttacagcaac tccaaacccc
     4561 acatatggat acttccaata tagccttgag aatgaagttt gtccaattgc cgccaaacta
     4621 gcaaaaagta acaaactagc aatacaaatt cttttcatca aattattgta taaccactat
     4681 ttgtttactg atctgttcac ctgttgctcc tgccacataa ccgatataca cgccaggtcg
     4741 cagtagagtg ttatctaact taacataaga aaccaagttg tcaagcggga attgcaatac
     4801 atttctgcct tgaacatctg taataataaa tcgagcatta ctttgaattg acttaagatt
     4861 tatttcaact acactgccac ttccaacagg atgcggataa acttgtatgg aattcggttt
     4921 actttgcggc atgctttgtt gaagcaacct caactcctct gtcgtcaact cttcgtttaa
     4981 ttcatttata ggttcaatgt aagcgccacc tgcaattagc tccataagta acttcgcttg
     5041 tatgccttct cggtcatttc gctgcgcttc ggttttaatg gaagaaaatt gttcttctgt
     5101 tgtttgcaac cacgtcaatg tatctcttga taagctcaaa cctagtgaca atagacttga
     5161 attgtatccg ttcagttcag catcgggaga ttcggttagt tcggcactta gcacttcggc
     5221 attatcatag tcacttaaat cctccaataa ttgcaccttg atgcttgttg ccagaggact
     5281 ttcatattca ttcagtaata tgttaaggct atctatgagt tcattttgta aataataaga
     5341 ggcttccata ttaaaacgta tatttttcaa aaaaccttca taatcaactc cagccggctt
     5401 caagtattca taaggattat atgtgagagg catcatcaga gttggtggtc gtctcttcgt
     5461 tgccttaggt gccgacatgc gtccactgcc gccgcttccg gatggaaagt tggtagagca
     5521 gtaatcatct agatttgtag ttgtgaaagt ttctacatcg ttacacccac tttcaaaaac
     5581 atcaccatca ggaagaggat caatagtatt cgaatagatt gcaggtgctg caaaaggtga
     5641 accacctgca tcatcatctt cgtgaaagga aaatttataa tagttcgtag ttgttatatc
     5701 actcgatgtt ttaatttgag tgttaatatc tgcaaagctt ggatctgtaa ataggttgtt
     5761 tgcaggtgag gtcaatgtcc cgcacgctcc ctgatttttt ttcatcgttg ccgttgtagc
     5821 acctgatatc aatgatatat catatttacc ttcttcgaaa gtattgcact tgatctccag
     5881 accatcgtca gtgtcgccat tgt
//
LOCUS       DAOQOU010000185         5858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8104_length_5858_cov_7.170946, whole genome shotgun sequence.
ACCESSION   DAOQOU010000185 DAOQOU010000000
VERSION     DAOQOU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8104_length_5858_cov_7.170946"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLM90_12625"
     CDS             complement(<1..646)
                     /locus_tag="PLM90_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ90273.1"
                     /translation="MQTIIGSGGAIGVPLAKELSVYTKDIRLVSRNPKKVNETDQLHP
                     CDLKDFSSIDAAIAGSDIVYVTVGFEYNIKVWQAVWPPFMRAVVDACIKHKAKLVFFD
                     NIYSYDPKAVGMLTEDAPLNPMTKKGRVRKSLVDMINKEVSAGNLTAMIVRAADFYGP
                     AVNTSILIEMAAKPMKAGKTANAMGNIDKIHTYTYTPDAGKATAILGNTPDAYNQ"
     gene            complement(709..912)
                     /gene="xseB"
                     /locus_tag="PLM90_12630"
     CDS             complement(709..912)
                     /gene="xseB"
                     /locus_tag="PLM90_12630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HMZ90274.1"
                     /translation="MQDKIGYSEAFDELQKIVAEIELGEISVDELSAKVKRASELIRI
                     CKAKLTSTEEEVDKILKELLDGE"
     gene            complement(902..2242)
                     /gene="xseA"
                     /locus_tag="PLM90_12635"
     CDS             complement(902..2242)
                     /gene="xseA"
                     /locus_tag="PLM90_12635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMZ90275.1"
                     /translation="MSEQSGTKKIFSLSEVARSIEKTLSERYSSAFWVTAEMNKLNLY
                     KHSGHCYPELVEKKDGKIVAQFKSTLWRADYLRINAVFQDVLKEPLKDGIKILFLARI
                     TFDAAHGLSLSIIDIDPNYTLGDLEREKLETIAKLKAEDLYAKNKQIALPQLPQRIAI
                     ISVETSKGYVDFLKVIEQNPWGYKFFHMLFPSLLQGDKAIDSIIQQLMLIEKVKHHFD
                     IIAIIRGGGGDVGLSCYNNFRLASAIANSSLPVLTGIGHATNETVCEMIAHYNAITPT
                     KLGEFILQQFHNFSVPVNDAKKFIADKALRILMEERTHLKSEVKLFRSMTENLLLRHN
                     NDVQSNIKLIRQVMNFRLRSEKNNIDYLKQQIHMRSAAFVLERNRELVGIERNVINMS
                     PALVLKRGYTITLHKGAVIKSFEELQQGDAIETITSEGVINSAITSIKKNNNAG"
     gene            complement(2297..2587)
                     /locus_tag="PLM90_12640"
     CDS             complement(2297..2587)
                     /locus_tag="PLM90_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90276.1"
                     /translation="MSDTSLAFMHALAENHGRDVSISLLDGTVHSGEIVGYFKGDGVI
                     GTSYIYKWHLLPKGVDNPTGLDFWGVPAGEYILHKHIEKVVFLSDGFTLVMK"
     gene            complement(2598..4562)
                     /locus_tag="PLM90_12645"
     CDS             complement(2598..4562)
                     /locus_tag="PLM90_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HMZ90277.1"
                     /translation="MKKVFESAHSRVSAATLLVALGIIYGDIGTSPLYVVKAVVGERP
                     VTEMLVYGAVSCVFWTITFQTTFKYIYLTLRADNRGEGGIFSLYALVRRYGKYLIIPT
                     IIGASALLADGIITPPISVASAVEGLSNINGLDNIPTMPIVIVIISILFIVQRFGTQI
                     VGRMFGPAMLTWFSMLFILGILQVIQHPDIIKALNPYYAYAFLTQYPGGFALLGGVFL
                     ATTGAEALYSDLGHCGKKNIRITWVFVKTCLITNYMGQAAWLMNSRYDSFLGSENPFF
                     MIMPEWFLLPGVLIATMAAIIASQALISGSYTLINEAMNLNFWPRIAVRQPSDTKGQI
                     YIPSVNIMLWIGCVLVVLHFKTSSHMEAAYGLAITITMMVTTYLLSYYLAFKLRWPKL
                     LIGLLVTLFASIEISFFIANIRKFPEGGYITIMVSSIFILIMYTIYKGRKISNRFTKF
                     VDLGKYAQQIKRLSEDDEVPRFSTHLVYLTKANYRHEIEEKIIKSIFSKKPKRADVYW
                     FLHIHRTEAPYTLSYNVSELIEDKVIKVNIHVGFRIQPRTELYFKLIVQALIDNKELN
                     LHLRDDGSTKYNYEPDFKFVVIEKFLSVENEFSLNEGLLLNGYFFLKQLGQSDEKAFG
                     LDKSDVVIEDVPLVYQPVNQVQLTRLDNNA"
     gene            complement(4659..5336)
                     /locus_tag="PLM90_12650"
     CDS             complement(4659..5336)
                     /locus_tag="PLM90_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ90278.1"
                     /translation="MSKAEILIIDDEPQIRKLLEITLESHEYKVWLATTGREGIVMAA
                     NHPPELILLDIGLPDMNGHEVLQDLRKWYNKAIIILSVLDGEEDIVRALDNGATDYLT
                     KPFRTAELLARIRSAIRRNQQEPLATIITIGDLAIDLPGRTVRLNGETVKLTSTEFNM
                     LALMAKNEGRVLTHQFILKEIWGVGYQSETQYLRVFVASLRKKIEQNPNQPSHIITES
                     GVGYRFM"
     gene            complement(5329..>5858)
                     /locus_tag="PLM90_12655"
     CDS             complement(5329..>5858)
                     /locus_tag="PLM90_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ90279.1"
                     /translation="QVDNLLNMSRLESGMLMPKYDWCDLQELVYTVIQKLEPGYTQHI
                     VVHAEDDLPLFKFDGGLMEQVLYNIIHNAIQYTPPSTTITVKLAHSDNGFNITIADDG
                     PGFPKELIQRAFDKFYRMPVSRTGGSGLGLSIVKGFVEAHKGNITLHNVDSGGSCFEI
                     YIPAETSYISNLKNE"
BASE COUNT         1718 a         1278 c         1112 g         1750 t
ORIGIN      
        1 cctgattata tgcatccggt gtattgccta aaatggctgt agcttttccg gcatcaggag
       61 tgtaagtata ggtgtgaatc ttatcgatat tgcccatcgc atttgccgtt ttgcccgcct
      121 tcatgggttt tgcagccatt tcaatgagta tactggtatt cactgcaggg ccataaaaat
      181 cggcggcccg aacaatcatg gcggtaagat ttcctgctga cacttccttg ttaatcatat
      241 ctacaagcga tttccttaca cgaccctttt tagtcatcgg atttaacggc gcatcctctg
      301 taagcatgcc aaccgctttg gggtcataac tgtagatatt atcgaaaaaa accagcttgg
      361 ctttatgttt aatacaagca tcaacaaccg cgcgcatgaa cggtggccac accgcttgcc
      421 aaacttttat attatactca aatccaacgg ttacatatac aatatcgctg ccggcgatcg
      481 cagcatcaat ggatgaaaaa tctttcagat cgcaaggatg tagctgatcg gtttcattta
      541 cttttttagg gtttctgcta accaggcgaa tgtctttggt atacactgaa agctccttcg
      601 ccaaaggcac gccaatagcg cctcctgaac ctataatcgt ttgcataatt gctgtagacg
      661 tattaaccgg gaaaatattg tatcaaaaat tgactattta tacacttact attcgccatc
      721 gagtaattct ttgagaattt tatctacttc ctcttctgtg gaagtgagtt tcgctttgca
      781 aattcgaatc aactcagatg cgcgctttac cttggcagaa agctcatcaa ccgaaatttc
      841 tcccagctca atctcagcca caatcttttg cagttcatca aatgcctcgc tatatcctat
      901 tttatcctgc attgttattc ttttttattg aagttattgc actattgatc acaccttctg
      961 aagtaatagt ttcaattgca tctccctgtt gaagttcttc aaagcttttg atcaccgctc
     1021 ctttatgcaa agttattgtg tatccgcgtt tcaaaaccag ggccgggctc atattgataa
     1081 catttcgttc aatgcccact aattcgcgat ttctctccag cacaaacgcc gccgatcgca
     1141 tgtgtatttg ttgtttcaga tagtcgatat tatttttttc agaccgcagt cgaaaattca
     1201 tgacttgccg tataagttta atattacttt gcacatcatt attgtgtcga agtaagaggt
     1261 tctctgtcat agaccgaaat aatttcactt ccgatttcaa atgcgtgcgc tcttccatca
     1321 agatgcgcag tgccttgtct gcaataaatt tcttcgcatc atttacagga acagaaaaat
     1381 tgtggaactg ctgtaaaata aattctccaa gtttagtcgg tgttattgca ttataatggg
     1441 caatcatttc acaaacagtt tcatttgttg cgtgcccaat tcccgtgagt accggcaatg
     1501 aactattagc aattgcagat gcaagtcgga agttattata acaggaaagc cccacatcac
     1561 ctccaccacc tctgataatg gcgatgatat caaaatgatg tttaaccttt tctatgagca
     1621 ttaactgctg aataatgctg tctatggcct tgtcgccctg caataaagag ggaaacaaca
     1681 tgtgaaagaa tttatatccc catggatttt gctcaatcac tttcagaaaa tcgacataac
     1741 ctttactcgt ttcaacgctg atgatggcaa tgcgttgagg cagttgcgga agcgctattt
     1801 gtttgttctt cgcataaaga tcttccgctt ttaatttggc aatggtttcc aacttttcac
     1861 gttccagatc gcccaaagta tagttggggt cgatatcaat aatcgacagc gacaatccgt
     1921 gagcagcatc gaaggtgata cgtgcgagga ataaaatttt aatgccatct tttaaaggct
     1981 ctttcagcac atcctgaaat actgcattaa tacgcagata atcagccctc cataaagtcg
     2041 atttaaactg tgctacaatt ttcccgtctt ttttttctac cagttcagga taacaatgac
     2101 ctgagtgctt ataaagattg agcttattca tttccgccgt cacccagaaa gcagaagaat
     2161 agcgttcgct cagtgttttt tcaatactgc gcgctacttc cgaaagagag aatatttttt
     2221 tagtgccgga ttgttcagac atgcgggcga ctaaaataca attttgccgt tgtccctaca
     2281 agtaaggcag acattgttat ttcataacca gcgtaaaacc gtctgacaga aataccacct
     2341 tttcaatatg tttatgtaga atatattcac ctgcaggcac gccccagaaa tccaagcctg
     2401 tgggattatc aaccccttta ggaagcaaat gccatttata aatgtaagaa gtgccaataa
     2461 cgccgtcgcc cttgaaatac ccaacaattt ctccactatg tacggtgccg tcgagcaacg
     2521 aaattgacac atctcgacca tgattttcag ccagtgcatg cataaatgcc aatgaagtat
     2581 cagacatata tgaaacttta agcgttatta tcaaggcgtg tcaactgcac ttgattcaca
     2641 ggttgataca ccaagggaac atcttcaatt acgacatcac tcttatcgag gccaaatgct
     2701 ttttcatcac tttgaccaag ttgtttgagg aagaaatacc cattcaacag gaggccttcg
     2761 ttcagcgaaa attcattttc tacagacaag aatttttcta tcaccacgaa cttgaaatcc
     2821 ggttcataat tatactttgt cgaaccatca tcacgcagat gaagattgag ttctttattg
     2881 tctatgagcg cctgaacaat gagtttaaaa tataattcag ttcgaggttg aatcctgaat
     2941 cctacatgta tatttacctt tatcactttg tcttctatca actccgatac attgtatgat
     3001 aaggtgtaag gcgcctcagt gcggtgaatg tgcagaaacc aatagacatc ggctcgttta
     3061 ggttttttgg aaaagataga cttgataatt ttctcttcaa tctcgtgacg ataatttgct
     3121 tttgtaagat aaaccaaatg tgtagagaaa cgtggcactt catcatcttc actcaagcgt
     3181 ttgatttgct gagcatattt acccaagtcg acaaacttgg tgaatctgtt actgattttc
     3241 cttcctttat aaatggtgta catgatgaga atgaatatcg aactcaccat aatagtgata
     3301 taacctccct cagggaattt gcgaatgttc gcgataaaga atgaaatttc aatagaggca
     3361 aataaagtaa ccagcaatcc gatgagcaat ttcggccatc tcaatttaaa tgccaggtaa
     3421 taagacaaca gataagttgt aaccatcatg gtaatggtaa tagcaagtcc gtatgctgct
     3481 tccatatgcg atgatgtttt aaaatgcaat accacaagca cgcatcctat ccaaagcatg
     3541 atatttacgc tgggaatata aatctgacct ttagtatccg aaggttgcct tacagcgatt
     3601 cgtggccaga agttaaggtt catagcctcg tttatcaagg tataactacc gctgatgaga
     3661 gcttgtgatg caatgattgc tgccatagtt gcaatgagca cccccggtaa taaaaaccat
     3721 tccggcatga tcatgaagaa cggattttcg cttcccaaaa aactatcata cctgctgttc
     3781 atcaaccatg ctgcctgtcc catataattg gtgatcaaac aggtttttac aaatacccat
     3841 gttatgcgaa tatttttctt cccacagtga ccaagatcag aatacaatgc ttcggcacct
     3901 gttgttgcga ggaatacgcc ccctaacaac gcaaatccac cgggatattg cgtcaagaat
     3961 gcataagcat aatagggatt caaggctttg atgatatcgg gatgctgtat cacttgcaag
     4021 attccaagaa taaacaacat cgaaaaccag gtaagcatgg cgggaccgaa cattcttcct
     4081 actatctgtg tgccaaatcg ttgtacaata aataaaatgg aaataatcac aattacaatt
     4141 ggcattgtag gaatattatc aagtccatta atattcgaca gaccttctac ggcggatgca
     4201 actgaaattg gcggggtgat gatgccatca gccaacaatg cggatgcgcc aataatggta
     4261 gggatgatca aatatttacc atagcgtctt acaagtgcat acaacgagaa gataccgcct
     4321 tcaccgcggt tatcggctcg caatgtcaga taaatatatt tgaaagtggt ttgaaaagtg
     4381 atggtccaga agacgcagga aacagcgcca tacaccaaca tttcagtaac cggtcgctct
     4441 cctacaactg cttttacaac atataaaggg ctggttccaa tatcaccata gataattccc
     4501 aatgccacca ataaagttgc tgccgatacg cgactatgcg ctgactcaaa taccttcttc
     4561 atgcacaaaa attctcaacg caaagttggc atgcgaggta ttggtaaaaa gtaaaatgaa
     4621 tcttccttta tataaagatt atataaagat ggcctaaatc acataaatcg ataacctacc
     4681 ccactttcag tgataatatg ggaaggttga ttgggatttt gctcgatttt ctttcgcaag
     4741 gaagccacaa acacgcgcag gtattgcgtt tccgactggt aacctactcc ccagatctcc
     4801 tttaaaatga actgatgcgt aagtacgcga ccttcatttt ttgccatcaa tgccagcata
     4861 ttaaattctg tggatgtaag cttgaccgtt tcgccgttca atctgactgt gcgacccgga
     4921 aggtcgatag caagatctcc tattgtaatt attgttgcca ggggttcttg ttggtttcga
     4981 cgaatggcgg agcgaatgcg tgccaataat tctgctgtgc gaaatggttt ggtgagataa
     5041 tctgttgcac cattgtccaa agctcttaca atatcttctt cgccatccag cacagataaa
     5101 atgatgatgg ctttattata ccattttctg aggtcctgca acacctcatg accgttcata
     5161 tcaggcaaac cgatatcgag caatattaat tcaggcggat ggttggcagc catcacaata
     5221 ccttcgcgac ctgtagttgc cagccatact ttatactcat gactttcaag tgtgatctcg
     5281 agcagttttc ggatctgcgg ttcatcatcg atgatcaaaa tttcagcctt actcattttt
     5341 caaatttgag atatatgaag tttccgcagg aatgtaaatt tcgaaacaag aacctcctga
     5401 atcaacatta tgcaaggtaa tatttccttt atgtgcttca acaaatcctt ttacgataga
     5461 caatcccaat ccactgccac cggtgcggga aaccggcatg cggtaaaatt tgtcgaaagc
     5521 gcgttggatt aactcctttg gaaatcccgg accgtcatct gcaatagtaa tattgaaacc
     5581 gttgtctgaa tgcgcaagct tgaccgtaat agttgtggaa ggcggtgtat attgaatggc
     5641 attatgaata atattataca atacctgttc cattaatccg ccatcaaatt tgaataaagg
     5701 aagatcgtct tctgcatgca caacaatatg ctgagtgtaa ccgggttcca gtttttggat
     5761 gactgtgtaa acaagttctt gtaaatcaca ccagtcgtat ttcggcatga gcataccact
     5821 ttccagtcgg ctcatgttta ataaattatc tacctgcc
//
LOCUS       DAOQOU010000186         5822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8189_length_5822_cov_5.872551, whole genome shotgun sequence.
ACCESSION   DAOQOU010000186 DAOQOU010000000
VERSION     DAOQOU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8189_length_5822_cov_5.872551"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..914
                     /locus_tag="PLM90_12660"
     CDS             234..914
                     /locus_tag="PLM90_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMZ90280.1"
                     /translation="MKRLLIICLGILSAFPMMAQNEGEINGKVIDGETGNSVPYAHCI
                     LLSSGAKVMEVEADDAGLFYFKPLKPGTYDLNVLFVGYDTAKLESIELASKGMVVLNV
                     EMSVGTILPPVYVGAPLVDKQVPEVVKTYKAKDIGHMAVDDAQDIIRLAPAVYEDEKT
                     GGMSIGGSREDATLYVVDGVKVIGPLYVPINSIAEVRIITGGIPANYGDVTGGVVEIT
                     TKGYSGVY"
     gene            1044..3560
                     /locus_tag="PLM90_12665"
     CDS             1044..3560
                     /locus_tag="PLM90_12665"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90281.1"
                     /translation="MKPVLFLLSLTASALLVQHVSSKKSAAPIRADQSSTADEWLYQQ
                     KAFPYDRPDRVAQRQAVADRLQQRNQMLMQKTEDVDDWHFIGPITIGGRILDIVCPVG
                     DDNTIYVGTASGGVLKSTNGGDSWANIFDAQSTLSIGDLAIDPNESNVIYVGTGEPGN
                     GVGSVTYDGNGIYRSNDAGITWTNIGLTNGGNTGRIAINPQNTDIIFTAMLGDLFENT
                     PDRGIYRTEDGGTTWEKVLYVNDSTGGIDVCINPENPDIVYAATWERVRRYNRRDYAG
                     QGSRVYRSEDGGDTWEMLTNGLPAGTQELSKISIAVAPSNPDYVYATVVGSNDKLKDI
                     YKSTDGGDTWIALNCTAEVTTTSQDNWFGGVRINPNDENEIYWVGFYVSRSTDGGSNW
                     TTITEDAHVDCHALYISPTDDNFRIMGSDGGLFFTYNDFISYTFEQNIPVTQLYTLDV
                     YPGDTSRLIGGAQDNGSFARDAGGDWAFVNWGDGVSTRFVPVDESSFYGNYQYGGYYG
                     IVGGNYMDLFGFDFDERFNWRSPMELNPLNPQTIYFGGNTIYRTTNYGESVSQISGDL
                     TDGSQGIGLTFGSIFTIHNAPADTNYIYVGTDDANVWRSDDYGDTWTSITDGLPYRYC
                     MSIETHPDNAQQAFVAFSGFRWAEDIAHIYMSENAGDTWTPIDGDLPDIPVNDIQVFT
                     IGDTTGLAIATDVGCYYSYDLGEHWFALGTAMPIVSVYEINYDTATNTLFAGTYGRGA
                     WKIEMPSAEPIVQSATQYADVSLTLWPNPVTDVANLHFNRKVSEADVSIVNMSGEAVF
                     RKVYSGVRDVSIPVDDLPQGTYMVVAEWDGKRMVTQMVHI"
     gene            3602..4147
                     /gene="pyrR"
                     /locus_tag="PLM90_12670"
     CDS             3602..4147
                     /gene="pyrR"
                     /locus_tag="PLM90_12670"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HMZ90282.1"
                     /translation="MQSRIILDSSKFRLTIERLCHQLIENHGDFSNACIIGVQPRGVN
                     LAKRIVWKLEELLPGTKIKFGILDTTFYRDDFRHRDKPLLAEETDIDFVIKGKEVILV
                     DDVFYTGRTIRSALDALLDFGRPEKIELLVLIDRRLSRHVPITPDYVGKTIDAVTSEK
                     VRVEWSENGIDDRVWIVPHKD"
     gene            4149..5099
                     /locus_tag="PLM90_12675"
     CDS             4149..5099
                     /locus_tag="PLM90_12675"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HMZ90283.1"
                     /translation="MSNLSVNHLISIKDLQPEDIHLILQTADNFKEVLNRPIKKVPTL
                     RDITIANIFFENSTRTRISFELAEKRLSADIINFSASASSVKKGETLIDTVNNILAMK
                     VDMVVMRHQSPGAPVLLSKHIKANIVNAGDGTHEHPTQALLDAFSMMEKLGESRGERL
                     KDLKGVRVAIIGDILHSRVAGSNIHALKMLGAEVTLCGPPTLMPRYIETLGVHVSHNV
                     KETLEWCDVANVLRIQLERQDIKFFPSLREYSLYYGIDKAMLDSINKDIVIMHPGPIN
                     RGVELSSDVADSKQSIILQQVENGVAIRMAVLYLLAGKLD"
     gene            complement(5167..5634)
                     /locus_tag="PLM90_12680"
     CDS             complement(5167..5634)
                     /locus_tag="PLM90_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HMZ90284.1"
                     /translation="MSTPQQILDNVKQFIQSVGVDPATCWNEQNKAYYVYKGSAKLEI
                     FVSSHPQNDGTTRHYLRIFSGLIKVPATNKEYFYRRCMEISDQSLGVKLTVMPNAAPE
                     NDWVYATYERDIAGMDYNETVTCVNDMGLWADWLDDQLKQEFGGGAGPGAPQQ"
BASE COUNT         1667 a         1097 c         1395 g         1663 t
ORIGIN      
        1 atcaggtatc aagaacaggt atcaagtatc aggtatcaag tattacagtt tccattcacc
       61 cactcaccac tcacccaccc gccaaaggcg aggcaagctc tctcctctct cctctctcct
      121 ctctcctctc tcctctctcc tctctccttt ctcctttctc ctatttctgt catctgtcat
      181 ttccgtgaca attgaacaaa acccataaga tacttttgat aaaaatccac attatgaagc
      241 gactacttat tatttgttta ggaattcttt ctgctttccc catgatggca cagaatgaag
      301 gcgagattaa cggaaaagtt attgatggtg aaaccggcaa ttcagtacct tatgcacatt
      361 gcattttatt gtcaagcggt gcaaaggtga tggaagttga agcggacgac gccggtttat
      421 tttatttcaa acctctaaaa cccggcacat acgatttaaa tgtattgttt gtgggttacg
      481 acacagcaaa acttgaatct atcgagttgg cgtcgaaagg tatggtagtg ttgaatgtag
      541 aaatgtcggt tggcactata cttcctcctg tttatgttgg tgcaccgcta gttgataaac
      601 aggttcctga agtagttaag acctataaag caaaagatat tggtcacatg gcagttgacg
      661 atgcgcaaga tattattcgt ctggctccgg cagtttatga agatgaaaaa acaggtggga
      721 tgtcgattgg tggttcgaga gaggatgcaa cattatatgt tgtggatggt gtgaaagtga
      781 ttggcccctt gtatgtacca attaattcga ttgctgaagt tcgcattatt acgggtggta
      841 ttccggccaa ttacggtgat gtaaccggtg gggttgttga gatcaccaca aaaggatatt
      901 ccggagttta ctgataccat aaattttaat acttgagtcc tgcgcaagca gctgtattgt
      961 ttgcttgaaa ccgccttcca tgctcagaag gcggtttttt tgtgcctgca gggagagtat
     1021 cgtatattta tacattcttt tatatgaagc cagtattatt cctactatcg ttgacagcat
     1081 cggctttgct tgttcaacat gtcagcagta aaaaatctgc tgcacccatt cgcgcggatc
     1141 aatcctcaac agccgatgaa tggctatacc aacaaaaggc ctttccgtac gacagacctg
     1201 atcgtgtggc ccagcgtcag gccgtggccg atcgcttgca acaacgcaat cagatgttga
     1261 tgcagaaaac agaagatgtc gacgattggc attttatcgg accaattact atcggtggca
     1321 gaattctaga tattgtttgt ccggttggtg atgataatac catttatgtt ggaactgcct
     1381 cgggaggtgt attgaaatct acgaatggag gagattcctg ggccaatatt tttgatgcgc
     1441 aatcaacatt atccattggc gatctggcta tcgatccaaa tgaaagtaat gtgatttatg
     1501 taggcacagg cgaacccgga aatggcgttg gttctgtgac ttatgacggc aatggtattt
     1561 atcgttccaa cgatgccgga atcacgtgga caaatatcgg gttgacaaat ggtggtaata
     1621 caggaagaat tgccatcaac ccacaaaata cggatatcat ttttacagcg atgttgggcg
     1681 atttgtttga aaatactccc gaccgcggaa tttatcgcac tgaagatggt ggcactacat
     1741 gggaaaaagt attgtatgtc aacgactcta caggtggaat agatgtatgt atcaatcctg
     1801 aaaatcctga tatcgtttac gcagcaactt gggaacgtgt gcgcagatat aacagacgtg
     1861 attatgcagg acagggtagt cgcgtgtata ggagtgaaga tggtggtgat acttgggaaa
     1921 tgcttacaaa cggattacct gccggcacgc aagaattgag taaaatatca attgccgttg
     1981 caccttctaa tcctgattat gtatatgcaa cagttgtagg aagcaatgat aaattgaaag
     2041 acatttataa atcaacagac ggtggtgata cgtggatagc attaaactgt actgcggagg
     2101 tgactaccac atcgcaggat aactggtttg gtggagtgcg cataaatccg aatgatgaaa
     2161 atgaaattta ttgggtagga ttttatgtat cgcgaagcac tgacggtgga tcgaactgga
     2221 caacaataac tgaagatgca cacgttgatt gtcatgcatt atacatttca ccaactgatg
     2281 ataatttcag aataatggga agtgacggcg gattattttt tacttataat gactttattt
     2341 cgtacacatt cgagcagaat attcctgtta cacaattata cacgctcgac gtgtatcccg
     2401 gagacacatc ccgtttgatt ggcggtgcac aagataatgg ctccttcgct cgcgatgcag
     2461 gaggagattg ggcttttgtg aattggggtg atggtgtgag cacgcgattt gttccggttg
     2521 atgaaagttc cttctatggc aattatcaat acggcggata ttatggtatt gttggcggga
     2581 attatatgga tttatttgga tttgattttg atgagcgatt taattggcgt agtccgatgg
     2641 aattaaatcc gcttaatccg caaacaatat attttggcgg gaatacaata tatcgcacaa
     2701 ctaactacgg ggaatctgtt tctcagataa gtggtgatct aacagatggc agtcagggaa
     2761 tcggtctgac attcgggtcg atatttacca ttcacaatgc gcctgccgac actaattata
     2821 tttacgtagg aacagatgat gcaaatgttt ggcgaagtga tgattatggc gatacctgga
     2881 catcgattac agacggactg ccatatcgct attgcatgag tatagagacg catcccgaca
     2941 atgcgcagca ggcatttgtg gctttcagtg gttttcgatg ggcagaagat atcgcacata
     3001 tctatatgtc ggaaaatgca ggtgatacct ggacacctat tgatggcgat cttccggata
     3061 ttccggttaa tgatattcaa gttttcacca ttggagatac tacaggctta gcaattgcta
     3121 ctgatgttgg atgctattat tcctatgacc tcggtgaaca ttggtttgca cttggcactg
     3181 ctatgcccat agtttcggtt tatgagataa attatgatac ggcaacaaat accctttttg
     3241 ccggtactta tggtcgagga gcctggaaaa ttgaaatgcc gagtgctgag cctattgtgc
     3301 aatctgctac gcaatacgct gatgtctcac tcacactttg gccaaatccg gtcactgatg
     3361 tcgcaaatct gcatttcaac cgaaaagttt ccgaagcgga tgtaagcata gtgaatatga
     3421 gtggggaagc agtgttccgc aaggtgtata gtggcgtaag agatgtgtct atcccggttg
     3481 atgatctgcc tcaaggcact tatatggtgg ttgcagaatg ggatgggaag cgcatggtga
     3541 ctcaaatggt gcatatataa tggttgctga tttgagtagg atgtgttaac tttgtgtttt
     3601 aatgcaaagc cgcattattc tcgatagttc caagttccga ctcaccattg agcggctttg
     3661 tcaccaatta atcgaaaacc acggagattt ttctaatgcc tgtattatcg gggtgcaacc
     3721 gcgtggagtg aatttggcca agcgcattgt atggaaatta gaggaattgc tgcccggaac
     3781 aaaaattaaa ttcggcatac tggatactac attttatcgt gatgatttcc gtcatcgcga
     3841 taaaccattg ctggcagaag aaacagatat cgattttgtg ataaagggta aagaggtgat
     3901 tttggttgat gatgtttttt atacaggaag aacaattcgc agtgccttgg atgcattgct
     3961 ggatttcgga agaccggaaa aaattgaatt actggtgctg atagacagac gattgagcag
     4021 acatgtgccc atcactcccg attatgtagg taaaactata gatgccgtta caagcgaaaa
     4081 agtgcgtgtt gagtggagtg aaaacggcat tgatgatcgc gtttggatag taccacataa
     4141 agattaatat gagtaacctg agtgtcaacc acctgatctc cattaaagac cttcaaccgg
     4201 aggacattca tcttattctc cagacagcag ataattttaa agaagtcctc aacagaccga
     4261 ttaaaaaggt tccgacactt cgcgatatta ccatcgccaa catatttttc gaaaacagta
     4321 cacgcacacg catttctttc gagctcgctg aaaagcgatt gagtgccgat atcattaatt
     4381 tttcagcatc ggcatcttcg gtaaaaaaag gtgaaacact tatcgatacc gttaataata
     4441 ttcttgccat gaaagtagac atggtggtaa tgcgacatca aagtccggga gcgccggttc
     4501 tactcagcaa gcatattaaa gctaatatcg ttaatgcagg tgatggcacg catgaacatc
     4561 ctactcaagc attacttgat gcatttagta tgatggaaaa attgggcgag tcgcgcggtg
     4621 aaagattgaa agatctaaaa ggtgtacgag tagcaattat tggcgacatc ttgcattcgc
     4681 gcgttgcagg cagcaatatt catgcactga aaatgttggg tgccgaagtc actttatgcg
     4741 ggcctccaac actgatgcct agatatattg aaacgcttgg cgtacacgtt tcgcacaatg
     4801 taaaagaaac cttggaatgg tgtgatgtag ctaatgtgtt gcgcattcaa ttagagcgac
     4861 aggatattaa attcttccct tcattgcgcg aatattcgtt gtactatggc atcgacaaag
     4921 cgatgttgga tagtatcaat aaagacatcg taataatgca tcccggacca atcaacaggg
     4981 gagtggaact gagcagcgat gtagcagata gcaaacaatc aataattctg caacaggttg
     5041 aaaacggtgt tgccatcaga atggcagtgc tttatttatt ggcaggtaaa ttggactaat
     5101 tattttcaaa ttttgaatag caaaaaaaat ccctgcaatg agcaaggatt ttttttatac
     5161 aaaaatttat tgctgaggag caccgggacc ggcacctcca ccgaactctt gtttcaattg
     5221 atcgtcgagc cagtctgccc ataagcccat atcgttgaca caggttacgg tttcattgta
     5281 atccataccg gcgatatcgc gttcataggt agcgtatacc caatcgtttt ccggtgcagc
     5341 atttggcatt acggttagct taacgccaag gctttgatcg ctgatttcca tgcatcgacg
     5401 gtagaaatat tctttgttgg ttgcaggaac tttaatcaac ccgctgaaaa tgcgcagata
     5461 gtgacgcgtg gtgccatcat tttgtgggtg actgctcaca aatatttcaa gttttgcaga
     5521 tcctttgtac acatagtacg ctttgttttg ctcattccag caggttgcag gatcgacacc
     5581 tactgattgg ataaattgtt tcacattatc cagaatttgt tggggggtgg acatgattgt
     5641 tgagtttagt tgatgaaggg ctaagataag gaaaaggaga gaggaaagag gagagaggag
     5701 agaggagaga ggagagagga gagaggagag ggtgagtggg tgagtgggtc agtgggtgag
     5761 tattattaaa ggcaaaaaaa aatcccgacc gcaggtcggg ataaaaatac ttgatacttg
     5821 at
//
LOCUS       DAOQOU010000187         5769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8324_length_5769_cov_6.683059, whole genome shotgun sequence.
ACCESSION   DAOQOU010000187 DAOQOU010000000
VERSION     DAOQOU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8324_length_5769_cov_6.683059"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1849)
                     /locus_tag="PLM90_12685"
     CDS             complement(224..1849)
                     /locus_tag="PLM90_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90285.1"
                     /translation="MQDIRINTRALRLTILMMLLFFTRFVFAQDGELDDSFGEGGLVK
                     TNLPVTDELCADAVVDADGFITIAGSAIQGGVRKALIARLNPDATPDVLFNETGCEVL
                     SDDGEIEESQVNAIALQSDGKIVAGGYVTFLGDTYGVLYRCNQNGGLDTTFGNSGIAK
                     FFSSYDQNYAQVKDIAVQEDGKIVFCGIEHSENTGFIDQFVTGRCLPDGTPDSSFGGA
                     GLVYCYFDWISGSAATIQVQTDGKILVGGRTNSDFALIRYLPNGVPDNTFGEWGEVTT
                     DIGYDDFITDLGILSDGKIIAVGYSKLPSSGNCYYKTRTSIACYNVDGSLYDGFSNDG
                     KLEMFFNYCRDEAYSVLIQPDDKIIIGGGTGKQNNSDSYNYYFLRLHKDGTRDYDFNS
                     DGYLESDMYENNYYDSYAKALLFQPSGKAVLVGYTDHWSISDSFISAIRVTTGVDSVA
                     LAQMNVYNQSSSDSTFSVWFSANLLYVKNVSTESIRLTICNISGSKLYTFQAESGMSN
                     YSLRNLPKGMYFVTGEDGKNVIAQKFIVSYAGY"
     gene            complement(1836..3857)
                     /locus_tag="PLM90_12690"
     CDS             complement(1836..3857)
                     /locus_tag="PLM90_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90286.1"
                     /translation="MNEKLKKLIQYSSHAGAFLALPAGLHGQVIYHNIDPDSVFSNDG
                     HVDIDFNADGMNDLTITEYYYYDLTFWTDYTMAWWGDFSVQISNFSGVISNVSYPSSY
                     GAMNLIEGFSINSAANWNTLDLIKLIHFSSYDSFIYAFNYDNWKGLNKYIGVKFLIGS
                     DTHYGWVRLGISSNGSVEGVKSYSLAVQDFAYEATADVPLLISNPTASVAEHVFISDV
                     YENENASDFRVQFDNAAYESTVSAYRIFLCPYTYAHPTPPSLAFLETLPPERYMEVEA
                     IGTPTYDVVLPATMTDINGAPVLGNYPHYYRALIVSMADGVMVSQNNVSLPSNTIYTK
                     LTAVPSPYGLSIDKPGSNCDITDFQVSFHKAGDESYISEYRVYILNDDHDIIAENDPS
                     YYVSVVPDGSLTYTVTPDVNTLIFEDTVPILFQNYYTEIMTVGDSIHASVASFESSRY
                     TSTYYGSTIGIEVELYCEQYDHTPVISLVDFTKTPADIRVQFNGPTKMSGVGQYRIYI
                     VPEEDIPNFTPEIAEHVPWISYTKIGDPEPYLDTVLSAELLDINGHTIALDKRYAVMI
                     GLFESSAPQKIAISLPSETFSFLSDESNIPLPYSYIYDNELYVISDINLPYMLNLYNM
                     AGEKVFTYEITSPITIIDLSDLPKGLYIIKGTTADEMPATKIFVGHAGY"
     gene            4132..4362
                     /locus_tag="PLM90_12695"
     CDS             4132..4362
                     /locus_tag="PLM90_12695"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMZ90287.1"
                     /translation="MWQEENNQLHKTFMFHNFSEAFAFMTRVAMLAEQHNHHPYWTNV
                     WNKVEIFLNTHDAGNIVTEKDHALAKAIDALL"
     gene            4385..>5769
                     /gene="ligA"
                     /locus_tag="PLM90_12700"
     CDS             4385..>5769
                     /gene="ligA"
                     /locus_tag="PLM90_12700"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HMZ90288.1"
                     /translation="MKYDQAAQHRLIALTEQLVKNLSDKNTNADTARDTIDDLKQVLH
                     FHDWKYYVDASPVIRDIDYDLLFKKLQSLEKSYPELLTEDSPTQRVARTLSDDFPTVS
                     HSVPMLSLENSYDENDLNTFDKRVRELTSTDNIIYCVEPKFDGSSIAVIYEGDMLLRA
                     ATRGDGIQGDEITINAKRMRSLPLRADFSKYGIHKVEIRGEVVIGKETFARINEKRES
                     EGQQILQNPRNSAAGALRVKDSEEIERRGLEAFMYHIAYAVDKDGNDLLGDQLRYHFD
                     NIEMLGKLGFKIPGKEKKKCHSVEEVHAFVQQWEQDRDDYTYEIDGMVIKVNDIAQQK
                     ICGATSHHPRWAIAFKFKAREQETELLSVEYQVGRTGAITPVAKVKTVYIGGVNVSSI
                     SLHNEDIIKEKDLHLHDIVVVQRAGDVIPYIDRVVLAKRDNSKIKTIDFIKECPSCNQ
                     AIYKPEEEAVYR"
BASE COUNT         1802 a         1226 c         1109 g         1632 t
ORIGIN      
        1 tgtttgatgt attttagcat gtgcttttta tctgaccata tgatcataca cccctaaact
       61 tttggtgcta cctttttgtc aagaaaaagg tagcggagag tgttttacga agaatgatct
      121 tgaagattac tttttacctt tttaaatgcg gcgaacttga aattcaattt tcagatgatg
      181 gacactttag taacatgacc acttttagaa aaattgttaa acttcaatag cccgcataac
      241 tcacaatgaa tttttgagca atcacattct tgccatcttc acctgtaaca aagtacattc
      301 ctttaggtag atttctcaaa gagtaatttg acatccctga ttcggcttgg aaggtatata
      361 atttggagcc ggatatattg catatggtca agcgtattga ttctgttgat acatttttaa
      421 catatagcaa attggcactg aaccatacag aaaaagtgct atctgaactc gattgattat
      481 acacattcat ttgtgccaat gcaacagagt ctaccccggt tgtaacgcgt atcgcagata
      541 taaatgagtc gctgatgctc cagtgatcag tgtagcctac aagcaccgct ttccctgagg
      601 gttgaaataa taaggctttg gcataactat cataataatt attttcatac atatctgatt
      661 ctagatagcc atcagagtta aagtcatagt ccctggtgcc atccttatgg agtctcaaga
      721 agtaataatt atatgaatca ctattgttct gtttaccggt gccaccccca atgattatct
      781 tatcatccgg ttgtatgagc acagaatatg cttcgtcgcg acaataatta aaaaacattt
      841 caagttttcc atcattgcta aaaccatcat ataagctgcc gtcgacatta taacatgcta
      901 tgctagttcg tgttttataa taacagttac cggagcttgg aagtttggaa taaccaacag
      961 ctatgatttt accatcgctg agaatgccaa gatcggtgat gaaatcatca tagccgatat
     1021 cggttgttac ctcaccccat tcaccaaatg tattatccgg tacaccattc ggtaggtatc
     1081 gaatcaaagc aaaatcacta ttcgttctgc cacccacaag aattttaccg tcagtctgca
     1141 cttgaattgt cgcggcgctt cctgagatcc aatcgaaata gcaatagaca agcccggctc
     1201 ctccaaagga actatccggt gtgccatcag gcagacatct tccagtgaca aattgatcta
     1261 taaatccggt attttccgaa tgttcaattc cacaaaatac aattttgcca tcctcctgaa
     1321 ctgcaatatc ttttacctgg gcatagttct gatcgtatga agagaaaaat ttcgcaatgc
     1381 cactgttgcc gaaagtggta tcaagtccac cattttgatt acatctgtag agaacgccat
     1441 aagtatctcc taaaaaagtt acatatccac ctgcaacgat ctttccatca gattgaagag
     1501 cgattgcatt tacttgtgat tcctcaattt caccatcatc ggaaagcacc tcgcagcctg
     1561 tttcattaaa taacacatca ggtgttgcgt ctggattcag gcgcgcaatt aatgctttcc
     1621 gaactcctcc ttgaatcgca ctgccggcaa tggttataaa accatctgca tctacaacag
     1681 cgtcggcgca taattcgtct gttactggga ggttagtttt aacgagccct ccttcgccaa
     1741 agctatcgtc taattcaccg tcttgagcaa acacaaaacg ggtaaaaaaa agtaacatca
     1801 ttaatatcgt gagccgcaat gccctggtat taattctaat atcctgcatg acctacaaaa
     1861 attttagttg ccggcatttc atcagctgtt gtgcctttaa taatatataa gccttttggt
     1921 aaatcactaa gatctataat cgtaatggga gatgtgattt cataggtgaa aaccttttcc
     1981 ccggccatat tatacagatt caacatatag ggaagattta tgtcgctgat tacatataat
     2041 tcattatcat agatatacga ataaggtaaa ggaatattac tttcatcact aagaaaacta
     2101 aatgtttcag atggcaagga gattgctatt ttctgtgggg cactcgactc gaaaagtcca
     2161 atcatcactg catatcgctt atctaatgcg attgtgtgtc catttatgtc aagtagttct
     2221 gctgataaca cagtatccaa ataaggctca ggatcaccaa tttttgtata gctgatccat
     2281 ggaacgtgct ctgctatctc aggtgtgaaa tttggaatat cctcctctgg gacaatatat
     2341 attctgtatt gcccgactcc actcatttta gttgggccat tgaattggac acgtatatct
     2401 gcaggtgtct ttgtgaaatc tacgagtgag atcacagggg tatgatcgta ctgctcgcag
     2461 tatagttcta cttcaattcc tatggtactg ccgtaataag ttgatgtata tcttgatgac
     2521 tcaaaactag caactgaagc gtgaatacta tcacctaccg tcattatctc agtataataa
     2581 ttctgaaata atattggcac cgtatcttca aaaatcaatg tgttcacatc cggcgtaaca
     2641 gtatatgtca ggctgccatc agggactacg cttacataat acgaagggtc attttctgca
     2701 atgatatcat gatcatcatt caagatatat acgcgatatt ctgatatata tgattcgtca
     2761 cctgccttat gaaagctgac ctgaaaatcg gtgatatcgc aattactacc gggtttatca
     2821 atcgacaaac catatggaga aggcacggca gttagcttgg tgtagatagt attacttggc
     2881 agtgaaacat tattttgcga caccatcacc ccatcggcca tagatacaat aagtgctcgg
     2941 taataatgcg gataatttcc aagcaccggc gccccgttga tgtctgtcat tgtggccggg
     3001 agcacaacat catatgtcgg ggttccaata gcttccactt ccatatatcg ctcaggaggc
     3061 agtgtttcta aaaatgcaag cgaaggaggt gtaggatgtg catacgtata cggacataaa
     3121 aaaatcctat aagcactcac tgttgattca tatgcagcat tatcaaactg tacgcgaaaa
     3181 tcggaagcgt tttcattctc atatacatcg gaaataaaaa catgctcagc aaccgatgcg
     3241 gtagggttgg aaattagcaa tggaacatct gccgtagctt cgtatgcaaa atcctgcacc
     3301 gccaaactat aactcttaac accctccaca cttccattac tagaaatacc caatctcacc
     3361 caaccgtaat gcgtatcact cccaatcaaa aattttacac cgatgtattt atttaagcct
     3421 ttccaattat catagttaaa tgcatagata aaagaatcat aacttgaaaa gtgaataagt
     3481 ttaatcaagt ccagcgtatt ccaattagca gcactgttga tagaaaaccc ttctatcaag
     3541 ttcatggctc cataagatga aggataggat acattggaaa tcactccgct gaaatttgat
     3601 atttgaacag aaaaatcacc ccaccatgcc atcgtataat ctgtccaaaa agtaagatca
     3661 taatagtaat actcggtgat ggtcaagtcg ttcataccat cggcattgaa atcgatatct
     3721 acatgtccat catttgagaa tacagaatcg gggtcaatgt tgtggtatat cacctgccca
     3781 tgcagtccgg caggcaatgc aagaaaggct ccagcgtgag aggagtattg tataagcttt
     3841 ttaagttttt cattcatggg gtgaatgttt taacaaatgt accaaatttc taccaattca
     3901 accacagcaa aaccgattga accatcggta tgcagctagg taattgtgag gatttgctga
     3961 tgagttgatg tgggccatta gaataaaagt tacccaccaa aatacccccc aaaacaacca
     4021 cgatcatctc tacacctcca cacttccaca tggagcgcag cgacatccag cctacgcgag
     4081 acttccatgc ctccccaccc ttttccttac ctttgaacaa acaacaatat catgtggcag
     4141 gaagaaaata atcaattgca taagactttc atgtttcaca atttttcgga agcctttgct
     4201 tttatgacgc gtgtggccat gctggcagag caacacaatc accaccccta ttggacaaat
     4261 gtgtggaaca aggtagagat attccttaac acgcatgatg caggtaatat cgttacggaa
     4321 aaagatcatg ctctggcgaa ggctatcgat gctttgttgt aactttttct gttaatttgc
     4381 aactatgaaa tatgatcagg ccgcgcaaca tcgattgatc gcgttaacag aacaattggt
     4441 aaaaaacctc tccgacaaaa atacaaatgc ggatactgcg cgcgatacca ttgatgatct
     4501 caaacaagta ttgcattttc atgactggaa gtattatgta gatgcatctc cggtaatcag
     4561 agatatcgat tatgacctat tattcaagaa attacaatcg ctcgaaaaat catacccgga
     4621 attactcacc gaagactccc ctacccagcg cgttgccaga acattgagtg atgatttccc
     4681 cacagtatca cattctgttc ctatgctgag tttggaaaac agctatgatg agaatgacct
     4741 gaatacgttt gataaacgtg ttcgcgaact gacgagcact gacaatatca tttactgcgt
     4801 tgaaccaaaa ttcgatggct cctccattgc agtcatatac gaaggtgata tgcttttacg
     4861 tgccgcaacg cgcggtgatg ggattcaagg tgatgagatc accatcaacg caaaacgtat
     4921 gcggtcattg ccgttgcgcg ctgatttctc taaatacggg attcataaag tagagatacg
     4981 cggtgaggtt gtaataggaa aagaaacctt tgcacgcatc aatgaaaaac gcgaaagcga
     5041 aggccagcaa atcctccaaa acccacgaaa ctctgccgca ggagcactgc gtgtgaaaga
     5101 ttcggaagaa atagaacgcc gcggactgga agcattcatg taccatattg cgtatgctgt
     5161 cgacaaggat ggcaacgatt tgcttggcga tcaattgaga tatcacttcg acaatatcga
     5221 gatgctcgga aaactcggat ttaaaattcc ggggaaggaa aaaaagaaat gccattcagt
     5281 tgaagaagtg catgctttcg tacaacaatg ggaacaagat cgcgacgact atacttacga
     5341 gatcgatggc atggtgataa aagtaaatga catcgcgcag caaaaaatat gcggtgccac
     5401 ttcgcatcat ccccgctggg caattgcatt taaattcaaa gcacgcgaac aggaaacaga
     5461 attattatct gttgaatatc aggtaggaag aacaggcgcg attacacctg ttgctaaagt
     5521 gaaaactgtt tatatcggtg gtgtgaatgt ttcttcaatt tcattgcaca atgaagacat
     5581 catcaaagaa aaagatttgc atctgcatga tatcgttgta gtgcaaaggg caggcgatgt
     5641 aattccttat atcgatcggg ttgtgcttgc aaaacgagat aatagtaaaa tcaaaactat
     5701 tgatttcatc aaagaatgtc catcgtgcaa ccaagctatt tacaaacctg aagaagaagc
     5761 tgtttaccg
//
LOCUS       DAOQOU010000188         5769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8325_length_5769_cov_5.907070, whole genome shotgun sequence.
ACCESSION   DAOQOU010000188 DAOQOU010000000
VERSION     DAOQOU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8325_length_5769_cov_5.907070"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLM90_12705"
     CDS             <1..465
                     /locus_tag="PLM90_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90289.1"
                     /translation="TTLDTITGVELATGYSPDIDLTESNDEPETKQEKYDPNVFALNA
                     WIAAMLGLALFLIPKMRDQHALHLIIALIGFGCMIGLMFNLKSALNDAKSSGGVVNVD
                     LPITFDMQPGYWIVTASFFLTLVADIVLWMHQRKVIADLHASRQPDNESNLS"
     gene            complement(526..1671)
                     /locus_tag="PLM90_12710"
     CDS             complement(526..1671)
                     /locus_tag="PLM90_12710"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ90290.1"
                     /translation="MKRIIYLVISMLAPVAMFAQVPTPPPPPVVPVEGTIKVEDEKVT
                     ITTVKGDIFIIDEFGVRKNGIMIAGDSEALREEIEDLQDEIDDIREESASLAPGSDAY
                     NDLMDEVSDLEDELTALSPESMYPDTLPGDSTALTIGNWRLIVKETGDDDDVDVTFGK
                     VPAIEEINDHYVDPFQTEWFLFDMGYNTYLNADYKTQLEDKYKQMEDLHGWGSFDVNL
                     HVWRSRVNMAKGFIDFNYGMSLEWHHFRYDQNFSILPNMDSLTLATETLDYDKNKFNT
                     THLAIPLLLGFETKPWDPENSFRMQFGYSPGLMLRGKTKHKLDGKKDIEFDDFNLAQF
                     RHELNYVIGYGDFNVYASYDLNSMFAEGEGPELHPFSVGLIIRRGFE"
     gene            complement(1690..2562)
                     /locus_tag="PLM90_12715"
     CDS             complement(1690..2562)
                     /locus_tag="PLM90_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90291.1"
                     /translation="MKITMQNYEEWMIDYIEGNLTPEQEKAMHEFLAFHPELRAELEL
                     FNHTRLTPETVAFPDKAALKHEEPAGRVITMFSWVRYAAAAAAVVAVFIGIRYFGGEQ
                     QQQGIQRYAQEQYDSNSINRNTTKTPIIESPVKQQQQEYAQTTTPEKNNIKSLPKEEV
                     KEQVKREINPIQGIDFASVDQIKSKQKDASLRDMMQQEPENEMVAMTDNQIKTGNAMN
                     STVISLNDNSTVVDWWVDAMAIGNEVGGVVNGVREAEINPFKKKEAGAQEVVKTKNIN
                     IPGFSYYSRKKTNH"
     gene            complement(2559..3047)
                     /locus_tag="PLM90_12720"
     CDS             complement(2559..3047)
                     /locus_tag="PLM90_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ90292.1"
                     /translation="MTPQEYNTTVDLWADGVYRFIRKSLNHDDDARDIVQNAFEILWK
                     NHENVQFDKAKSYLFSTAYHNMIDHLRKQKRTDYVEEMDEEARGGTAEAQVDLKEVIE
                     KALSRLPEVQKNCVLLRDYEGYSYEEIGEVLNLSESQVKVYIFRARQTMKEYLVSIHY
                     VI"
     gene            3172..4020
                     /locus_tag="PLM90_12725"
     CDS             3172..4020
                     /locus_tag="PLM90_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ90293.1"
                     /translation="MSILFPTNFSDQSVETFGFALHLAKKFNLDITLLHVYPLPMTLP
                     SMDEGRMAEVTDDMIQATIAANEERLNLFKQRLSDHYANAHPDVVRVNGILRMGFVGE
                     EVARCAEEEHADFVVISAKKATGLKRFLGGSDVASIIRRCQLPIFTVPEHYSFHNIDK
                     IGYATDLTFSDNEVIARLLVLGEKFGATVKCFHVHDSNLDIENAIIKEFIEQYKDDAN
                     ARKISFDLVDNINIHDGIDYYVKNNNIELLAVLKQKKYWLEIFESSMTKQLVFHEKIP
                     MLIYHE"
     gene            complement(4702..>5769)
                     /locus_tag="PLM90_12730"
     CDS             complement(4702..>5769)
                     /locus_tag="PLM90_12730"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ90294.1"
                     /translation="KNGKCTGILMDNAMELAKRKMPTKSSKFLTNALARAQSDCFAVG
                     LTSVQDAGLELHEIMTLKTAADNGKLQMRVYAMATAEPKTFDYFMENGPMYGDRLNVR
                     AFKFYADGALGSRGAGLLKPYSDDPDNYGIYYYPYDSLSWQAKRVYNAGFQMCTHAIG
                     DSANRLILDIYGATLQGKNDLRWRVEHCQVVSKSDINKFGQFSILPSVQPTHATSDMY
                     WATDRLGADRLKTAYAYKDLMKQNGMLIDGSDFPVESINPIYGFYAAVARKDLKMYPD
                     KGFQKENAIGRNEALKAMTIWAAYGAFEEGVKGSLEVNKYADFVILDKDILAIPEPEI
                     PTAKVLMTVVGGDVVYERKSE"
BASE COUNT         1574 a         1279 c         1180 g         1736 t
ORIGIN      
        1 accactttag ataccattac aggcgttgaa ttagctacag gttatagccc ggatatcgat
       61 ctgactgaat ctaacgatga acctgaaaca aagcaggaaa aatacgatcc gaatgtgttt
      121 gcactcaatg catggattgc tgcaatgctg ggtttagcct tatttcttat tccaaaaatg
      181 cgcgatcagc atgccctgca tctcatcatt gcattaattg gattcggatg tatgatcggg
      241 ctcatgttca atttaaaaag tgcactcaac gatgctaaat cttcaggtgg cgttgtgaat
      301 gtagaccttc caattacctt cgatatgcag ccgggatatt ggattgttac tgcatctttt
      361 tttctgacat tggttgctga cattgtgctt tggatgcatc aacgaaaagt aattgctgat
      421 ttgcatgcat cgaggcaacc cgataatgaa tccaatctat cataaaaaaa agaggcaggg
      481 tcctcaccct acctcttatt gaccaaacct atacagaaat tttaattatt caaatccacg
      541 acgaatgatg agtcccactg agaaaggatg caactcagga ccttcacctt cggcgaacat
      601 gctattcaga tcataagacg catacacatt gaaatcgccg taaccaatca cataattcaa
      661 ttcatgtctg aactgtgcga ggttgaaatc gtcaaattct atatctttct taccatccag
      721 tttatgtttt gttttaccgc gaagcattaa gccggggcta tagccgaatt gcatgcggaa
      781 gctgttttca ggatcccaag gttttgtttc aaacccgagg agtaagggaa ttgcaaggtg
      841 ggtggtattg aatttatttt tatcgtaatc caatgtttct gttgccaatg tcaatgaatc
      901 catgtttggc aggatactga aattctgatc atagcggaaa tgatgccatt cgaggctcat
      961 accataattg aaatcgataa atcctttagc catatttacc cttgaacgcc acacgtgcag
     1021 atttacatca aaacttcccc aaccatggag gtcttccatt tgtttgtatt tatcttcgag
     1081 ttgagtttta taatcagcat ttaaataggt attatatccc atatcaaata agaaccattc
     1141 agtttgaaac ggatccacat aatggtcatt aatttcttca atagcgggca ctttaccgaa
     1201 tgtaacatct acatcatcat catcacctgt ttctttaacg ataagtcgcc agttaccaat
     1261 tgtcaatgcg gttgaatcac ccggcaaagt atccggatac atggattcag ggctgagtgc
     1321 ggtcagttca tcttcaagat cagaaacttc atccatcaga tcattataag catctgaacc
     1381 aggagccaat gacgcagatt cttcacggat atcatcaatc tcatcttgca aatcttcaat
     1441 ttcttcacgc agtgcttcag aatcaccggc tatcataatt ccatttttac gcacaccaaa
     1501 ttcatcgata ataaagatgt cgcctttcac ggttgtaata gtaaccttct catcttcaac
     1561 tttgatagtt ccttccacag gcacaaccgg tggaggaggt ggagtgggca cctgagcgaa
     1621 catggcaaca ggagccagca tgcttataac taaataaata atccttttca taatatacga
     1681 tttatgcgat taatgatttg tttttttacg actgtaataa gaaaatccgg gtatattgat
     1741 attttttgtt ttcactactt cttgtgcgcc tgcttctttc tttttaaagg gattgatctc
     1801 agcttcgcga acaccattta ctactcctcc tacttcattt ccaatggcca tggcatctac
     1861 ccaccagtcg acaacggtgc tgttatcatt taatgaaata acagtactat tcattgcgtt
     1921 gcctgttttg atttgattat cggtcatggc aaccatttca ttttcaggtt cctgttgcat
     1981 catatcgcgc aagcttgcat ctttttgctt gcttttaatc tgatcaaccg acgcaaaatc
     2041 aattccttgt atcgggttaa tttccctttt tacttgttct ttaacttcct ctttaggaag
     2101 gcttttaata ttattttttt ccggtgttgt tgtttgcgca tattcttgct gctgttgttt
     2161 aaccggcgat tctataatcg gtgtttttgt ggtattgcga ttgatcgaat tgctgtcata
     2221 ctgctcctga gcatagcgtt gaatgccttg ttgctgttgt tcaccaccga aatatcgaat
     2281 gccgatgaaa actgcaacta ctgcagcggc ggcagcagca tagcgcaccc aactgaacat
     2341 ggtaatcacg cgtcctgcgg gctcttcatg cttaagcgca gccttatcag ggaaggcaac
     2401 agtttcaggt gtaaggcgtg tatgattaaa caattccagc tcagcacgta gttcaggatg
     2461 gaaagccaaa aactcatgca tggctttttc ctgctccggt gtcagattac cttctatgta
     2521 atcgatcatc cattcctcgt aattctgcat tgtgatcttc atataacgta atgtatgctt
     2581 accaaatatt ctttcattgt ttgccttgcc ctgaaaatgt acactttcac ttggctctcg
     2641 ctcaaattca gcacctctcc tatctcctca taactgtatc cttcataatc gcgaagtaat
     2701 acacagtttt tttgcacttc cggcaggcga ctgagggctt tttctatcac ctctttaagg
     2761 tcgacctgcg cttctgcagt gcctcccctc gcctcttcgt ccatctcctc gacataatcg
     2821 gtgcgttttt gtttccgcaa gtgatcgatc atgttatggt aggccgtgct gaataaataa
     2881 ctcttagcct tgtcaaactg aacgttttcg tggttcttcc acaagatctc gaaagcattc
     2941 tgaacgatat cccgggcatc gtcgtcatga ttgagactct tgcgtatgaa ccggtagaca
     3001 ccatcagccc acagatctac tgtcgtgtta tattcctgag gtgtcatcct gcaataaggc
     3061 gtatagggtt accaaaaagt tacagttatc ttaaaatttt tttcaaaagg cgacaggaat
     3121 cactccttag ccgctgattt ggatgtaaat ttacagaaac gaagaaccga tatgagcatc
     3181 ctatttccta ctaacttcag cgaccaatcg gttgaaacct ttggttttgc gctacatctg
     3241 gcgaaaaagt tcaatctgga cattacgctt ttacatgtat atcccctgcc tatgaccctg
     3301 ccttctatgg atgaagggcg tatggcggaa gtcaccgatg atatgattca ggctacaatc
     3361 gccgccaacg aggagcgcct gaatctgttc aaacagcgtc taagcgacca ctacgctaat
     3421 gcgcatccgg atgttgtgcg ggtaaatggc attttacgta tgggctttgt gggcgaagaa
     3481 gttgcccgct gcgcagaaga ggaacatgcc gattttgtgg tgatcagtgc gaaaaaggca
     3541 acgggattaa agcgcttttt ggggggaagt gatgttgcct caattattcg tcgctgccaa
     3601 cttccgattt tcacggtacc ggaacattac agtttccata atatcgataa aatcggatat
     3661 gctactgacc tcactttttc ggacaatgaa gtgattgcaa gattacttgt gctcggagag
     3721 aaatttggcg ctacggtaaa atgttttcac gtgcacgatt caaatctgga tattgaaaat
     3781 gccatcatta aagaatttat cgagcaatat aaagatgatg ccaatgccag aaagatatct
     3841 ttcgacctgg ttgataatat caatattcac gatggtattg attactatgt aaaaaataat
     3901 aatattgaat tactggcggt gctaaaacag aaaaaatact ggcttgaaat atttgagtct
     3961 agtatgacaa agcagctggt gttccatgag aagataccga tgttaattta tcatgaatag
     4021 tagagaggag agaggaaaaa ggagagagga gaaatgtggg aatgccagcc tcgccgcagg
     4081 cgggtgggaa tgccagcctc gccgcaggcg ggtgagaatg tgagaatgcc agcctcgccg
     4141 caggcgggtg ggaatgtggg aatgtgggaa tgccagcctc gccgcaggcg ggtgagaatg
     4201 tgagaatgtg ggaatcaata gcgttttcaa ctcctttaaa attgcaattt tcataacctt
     4261 taattaaaac cttaagaata attcatcatc aaatgcattt aattccccct ctctgcgacg
     4321 ccaggagtgg agaggaggtc agggggtgag gcggggttcg cgctaagacg caaagatgca
     4381 aagtcgcaaa gttggcattc acgagtcacg attcacgaca ttagtatcta gaaccaagta
     4441 tctagtatct agacttggaa tctggtatca ggtatcaggt atcaagtatc aagtatcagg
     4501 taaatgcaaa ctcgcatcac attctcgatt cttaggtact caccacactc acgagtcacg
     4561 attcacgatt cacgaccggt agtgagtagt gggtattgag tagtgagact aatgcaacca
     4621 ttcgctcgca aagcgagcga atttgcatag tgcccactca ccactcaccc acccgcctat
     4681 cggcgaggca agctcaccca ctcactcaga ctttctttcg tagacaacat cacctcccac
     4741 aactgtcatc aacactttcg ctgtaggaat ttcgggttcg gggatggcca ggatgtcttt
     4801 atcaaggatt acgaaatcgg cgtatttatt tacttcaaga cttcccttca caccttcttc
     4861 aaatgcgccg tatgctgccc aaatggtcat tgcttttaat gcttcatttc ggccgatggc
     4921 attttctttt tgaaaacctt tatcgggata cattttcaaa tcttttctgg caacagctgc
     4981 ataaaatcca taaatcgggt ttattgattc taccggaaaa tcgctgccat cgatcaacat
     5041 accattttgc ttcatgaggt ctttgtatgc atatgccgtt tttagtctat ctgcaccaag
     5101 tctgtctgtt gcccaataca tatcactggt agcatgtgtg ggttgaacag atggaagaat
     5161 ggaaaattgt ccgaatttat taatgtcaga tttgctaact acctgacaat gttccactcg
     5221 ccaacgcaga tcattttttc cttgtaatgt tgcgccataa atatcgagga taagtctatt
     5281 agctgaatcg ccaatagcat gcgtacacat ttggaatccg gcattataaa cgcgttttgc
     5341 ctgccagctt aaagaatcgt aagggtaata ataaatacca taattatccg gatcatcgct
     5401 atatggtttt aagagtcctg cacctctgct gccaagtgcg ccatcagcat aaaatttaaa
     5461 tgcgcgcaca tttaaccgat cgccatacat gggaccgttt tccataaaat aatcgaatgt
     5521 tttgggctct gcggttgcca tcgcatacac acgcatttgc aactttccat tatccgcggc
     5581 agttttcaaa gtcataatct cgtgcaattc gaggccggca tcctgcacgc tggtgaggcc
     5641 gacagcaaaa caatcgcttt gtgcacgcgc taatgcatta gtcagaaatt tagaactctt
     5701 ggttggcatt tttcgcttag cgagttccat cgcgttatcc attaatatac ccgtgcattt
     5761 cccattttt
//
LOCUS       DAOQOU010000189         5670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8581_length_5670_cov_4.757079, whole genome shotgun sequence.
ACCESSION   DAOQOU010000189 DAOQOU010000000
VERSION     DAOQOU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8581_length_5670_cov_4.757079"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="groEL"
                     /locus_tag="PLM90_12735"
     CDS             complement(<1..923)
                     /gene="groEL"
                     /locus_tag="PLM90_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMZ90295.1"
                     /translation="MAKIIKFDVEAREKLKSGVDQLANAVKVTLGPKGRNVVIEKKYG
                     APNVTKDGVSVAKEIELEDPIENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIMAAG
                     LKNVAAGANPMDLKRGIDKAVETVVTELKKMAQNVGNDNKKIEQVAAISANNDAEIGK
                     LIADAMAKVSKDGVITVEEAKGTDTYSEVVEGMQFDRGYLSAYFITNTDEMEAELERA
                     YVLIYDKKISTMKDLLPVLEKVAQQGAQMLIIAEDVDGEALSTLVVNKIRGTIKVCAV
                     KAPGFGDRRKEMLQDIAILTGGTVISEEQGY"
     gene            complement(966..1235)
                     /locus_tag="PLM90_12740"
     CDS             complement(966..1235)
                     /locus_tag="PLM90_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMZ90296.1"
                     /translation="MTIKPLADRVIVLPAPAETTTKGGLIIPDTAKEKPQRGVIVAAG
                     PGKKDEPVTVKVGDTVLYGKYAGTEINVDGVEYLIMRESDVFAIL"
     gene            complement(1476..2744)
                     /locus_tag="PLM90_12745"
     CDS             complement(1476..2744)
                     /locus_tag="PLM90_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMZ90297.1"
                     /translation="MLFDAAPLAERMRPKSLEEYIGQQHLVGEGQVLRKMAETGRLVS
                     MILWGPPGVGKTTLANILANAADLPFFSLSAVSAGVKDVKEVIEKAQRSGKIVLFIDE
                     IHRFNKAQQDSLLGAVEKGLIILIGATTENPSFEVINALLSRCQVYVLKELERADLQL
                     LVERAVAKDAEISKRKYIIKEDSALLKLSGGDARKLLNIIELIDETVQSSKVEITDAL
                     VTDIVQTKTAQYDKNGEMHYDLISAFIKSMRGSDPNAAMYYLVRMIDGGEDPKFIARR
                     MLILASEDIGNANPNALLLATACFQSVTMLGYPECSIPLAQTVVYLATSPKSNASYMA
                     LMKGYELVRETGDLPVPLAIRNAPTKLMKQLDYGKDYQYAHDHPLNFANMEFLPEEIS
                     GTKLYEPGKNAREEEFRKFLRDRWKEKYGY"
     gene            complement(2823..3227)
                     /locus_tag="PLM90_12750"
     CDS             complement(2823..3227)
                     /locus_tag="PLM90_12750"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ90298.1"
                     /translation="MKIGRRRKTEAEVFSASMNDIMFFLMLFFLIISTLANPNVIKVM
                     LPNAENVETTYNKQEVSLTVTADKQFFVNDSPVAFEMLESSLISATGGSEDKVVLLRA
                     DATLTIQDLVDVLQIGAKNNLHIVMNTAKSSN"
     gene            complement(3227..3916)
                     /locus_tag="PLM90_12755"
     CDS             complement(3227..3916)
                     /locus_tag="PLM90_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ90299.1"
                     /translation="MLSTLLQIPTVTSTPASTTPPAVDQELHIFELLVKGGPIMIPIA
                     ILLVLAIYLWIYKSITISQLSRMDERMIPAIKDQLTSGNLSSAEAYVRNINTAQARVI
                     DSGIATLGRSMREIESNLETASEIEIMQMEKHLGYMGIIAGIAPMLGFIGTIAGVIKI
                     FYNISVSNDISISIISSGLYEKMITSGFGLLTGIIAYGGYHLLNMKIDRTALKLQRNS
                     FEFIRIINKSE"
     gene            complement(4173..4943)
                     /locus_tag="PLM90_12760"
     CDS             complement(4173..4943)
                     /locus_tag="PLM90_12760"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF008877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HMZ90300.1"
                     /translation="MEPTVLIPQTQDYSAYTPEHFKVWEILFDRQMALLKDRAAEVFM
                     QGIDLIHFSADRIPKFDEFNIRLETLTGWEAVVVPGLIDNKPFFELLKNRRFPASTWL
                     RSMEQLDYLEEPDMFHDVFAHLPLLTNKTYCAYLYGLSKIALSYIDNPVAVELISRIY
                     WYTVEFGLIRDNGLLRIYGSGILSSQGESIYCLKSGIPSKRVDFNVEKILDTPYIKDK
                     FQSIYFVIDSCLQLFESLPDIEQGIARRIEHPELFVAN"
     gene            complement(5172..>5670)
                     /locus_tag="PLM90_12765"
     CDS             complement(5172..>5670)
                     /locus_tag="PLM90_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90301.1"
                     /translation="GRISIANQYRDGYLLFTAGDYNYAYQKFNWLRNRVESEYTNGLP
                     DGVCKQLYISSITLKEISDILATVYCQYPFWIDDREAVYDINYGTQFLIFPNPARTIV
                     TLGSLNGLSANYRIFSLSGEQMSEGVMEGSVTLDIANYPKGVYMAIFSDTTGNIIETQ
                     KIILQ"
BASE COUNT         1561 a         1219 c         1185 g         1705 t
ORIGIN      
        1 ttataacctt gctcttcgct gataacagtt ccaccggtaa gaatggcgat atcctgcagc
       61 atttccttgc gacgatcgcc gaagcccgga gcttttactg cacatacttt aattgttccg
      121 cgaattttat ttactacgag tgtagagagt gcttcaccat ctacatcttc tgcgatgatt
      181 aacatctgtg caccttgctg agctaccttt tccaacacag gaagaaggtc tttcatcgtg
      241 ctgatctttt tgtcgtagat caatacataa gcgcgttcga gttcagcttc catttcatcg
      301 gtgttagtga tgaagtatgc tgataaatat ccgcgatcga attgcatacc ttctactact
      361 tcagagtaag tatcagttcc ttttgcttct tcaacggtga tcacaccatc tttagaaact
      421 ttggccatag catcagcgat cagtttaccg atttcagcat cgttatttgc agaaatggcc
      481 gctacttgtt caattttttt attgtcgttg cctacatttt gagccatttt cttcaattct
      541 gttaccacag tttcaacagc tttgtcgata ccgcgtttca gatccattgg attagcacct
      601 gcggcaacgt ttttcaaacc ggcagccatg atagcctgag caagcacagt tgcagtagta
      661 gtaccatcac cggctacatc gctggttttg ctggctactt ctttcaccat ttgggcgccc
      721 atgttttcga tagggtcttc gagttctatt tctttggcaa cagagacacc atcttttgta
      781 acgtttggag cgccatattt cttttcgata accacattgc gtcccttagg gccgagggtt
      841 acttttacag cattggcgag ttggtctaca ccacttttca gtttctcgcg tgcttccaca
      901 tcaaatttta taatcttagc catgttctat atttaattgt tgtttgtcag ataaggtttg
      961 aaagatcaaa gaatggcgaa aacatccgat tcgcgcatga tcaggtattc aacaccgtca
     1021 acattgattt cggtgccggc gtattttccg tataacacgg tatcgccaac tttcacggtt
     1081 acaggctcgt cttttttacc gggaccggca gcaacgatta cgcctctttg tggtttctct
     1141 ttggcagtgt cgggaatgat caatccgcct ttggtagtag tttcagcagg tgccggcaat
     1201 acaattactc tgtcggcaag tggtttgatg gtcagcttag atttagccat atgaatacgt
     1261 atttatttga ttttaaatga tttgacaggt cgccttcagc aaattccttg ccttggtata
     1321 gaaacggcaa ttttgacgct tggcagggac aaaatagcag gttttggcag attcatcggc
     1381 aggcagcttg tcacgcccta cccttcaaat tggtatactt tttgcctctt ttttccggaa
     1441 aacaggctga aacgtcctac cagcatagct ttcagtcaat acccatactt ctccttccac
     1501 ctgtcgcgca gaaattttcg aaactcttct tcacgggcat ttttgccggg ttcatagagt
     1561 ttggtgccgc ttatttcttc gggaaggaac tccatgtttg cgaaattcaa aggatggtca
     1621 tgcgcatatt ggtaatcttt accataatcc aattgcttca tgagtttggt aggtgcattg
     1681 cgaatagcaa gaggcactgg cagatcgccc gtttcgcgga caagttcata gcctttcatc
     1741 aatgccatat aactggcatt gctctttggt gatgtagcaa gataaacaac cgtttgagcc
     1801 aatggaatac tgcattcagg ataacccaac atggtaaccg attgaaagca tgccgttgct
     1861 aaaaggagtg catttggatt ggcattgcca atatcttcac ttgcaagaat gagcatgcga
     1921 cgagcaataa atttcgggtc ttcaccgcca tcgatcatac gcaccaaata atacatggca
     1981 gcattgggat cacttccgcg catactttta ataaatgcgg aaataaggtc ataatgcatc
     2041 tccccattct tatcgtattg tgctgttttt gtctgcacaa tatcggtaac caaagcatca
     2101 gtgatttcca ctttactgct ttgcaccgtt tcgtcgatga gttcaatgat attgagcaat
     2161 tttcttgcat cgccgccgct taatttcaaa agcgcgctat cctcttttat tatatacttt
     2221 cgcttagaaa tttcagcatc tttcgcaact gctctttcaa ctaataattg caaatcagcg
     2281 cgttcaagtt ctttcaacac atacacctga catcgcgata gcaaggcatt aattacttcg
     2341 aatgatggat tttccgtggt ggcaccaatt aggatgatga ggcctttttc aacggcaccg
     2401 agcaatgaat cttgttgtgc tttgttgaag cgatgaattt catctataaa taaaacaatt
     2461 ttcccgctgc gttgtgcttt ttctatcact tcttttacgt ctttcacacc ggcagaaaca
     2521 gcgctgagcg agaaaaacgg cagatccgca gcattggcca gaatgtttgc aagggtagtt
     2581 tttcctacac ccggaggccc ccataatatc atgctgacaa gtcgccctgt ttcagccatt
     2641 ttgcgcaata cctgaccttc accaacgagg tgttgctgac caatgtactc ttccagagat
     2701 tttggtcgca tgcgttcggc aagtggtgct gcgtcaaaaa gcatgatgca aagctagcat
     2761 tcccctgaaa ataaaacgac ccgacaattc gccgggtcgc ataaaaagta tttcggtgtt
     2821 acttaattcg atgatttcgc agtattcatt acgatatgca gattattttt tgcacctatc
     2881 tgcaatacat caacaagatc ttgaatggta agggttgcat cggcacgaag caataccacc
     2941 ttgtcctcag aacctccagt agcgctgatg agggatgatt ccaacatttc aaatgcaaca
     3001 ggactgtcgt ttacaaaaaa ttgtttatcg gcagtaacgg tgagtgacac ctcttgtttg
     3061 ttgtaggtag tttctacatt ttcggcgttc ggcagcatca ccttgatcac attgggattc
     3121 gccagtgtgg agatgatgag gaagaacaac atcaaaaaga acatgatgtc attcatggat
     3181 gccgaaaaca cctctgcttc cgtttttctt cttctcccga tcttcattat tcagatttat
     3241 tgatgatgcg aataaattcg aatgagttac gctgcaattt caaggcggtg cgatctattt
     3301 tcatattcag caagtgataa ccaccgtatg ctatgatacc tgtgagcaga ccaaatccgg
     3361 aggtaatcat tttttcatac agacctgaag aaatgatact gatggagatg tcgttagata
     3421 cagagatatt ataaaagatt ttgataaccc cagcaatggt tccgatgaat ccgagcatcg
     3481 gtgcaatacc ggctatgata cccatgtacc cgaggtgttt ttccatttgc atgatctcga
     3541 tttcagatgc ggtttccaga ttggattcaa tctctcgcat actgcgaccc aaggtagcga
     3601 tacctgaatc gatcacacga gcctgagcgg tattgatgtt gcgcacatag gcttctgccg
     3661 aagaaagatt tccactggta agctgatcct taatggcagg aatcatgcgt tcatccatgc
     3721 gtgagagttg actgatggta atggatttat agatccacaa atagatagcc agcaccaata
     3781 aaatggcgat aggaatcatg atgggaccac ctttcaccaa taattcaaaa atgtgtaatt
     3841 cctgatcaac agcaggtggt gtagtgcttg caggagttga tgttacggta ggtatttgca
     3901 gtaaggtact taacatatgt tcagttgctt taaataatgt aaagaattat gccaattcta
     3961 tgccaacaaa gttacatgat taggatgccg atgaaagtga attatccata atgcttgcgg
     4021 cagctgatat tgaattgtct ataataatac tcaataggca ataagcgaag ttgcaagata
     4081 aacgcctctt gtcctcaaat tcgtataaac agcattcagg gggctgaagg aacttcaacc
     4141 cggcaagcgc aaaaaaccgc tacatcaagt gattaattcg caacaaataa ttcaggatgt
     4201 tcaatgcgtc gggcgatacc ttgttcgata tccggaaggc tttcgaacaa ttgcaggcag
     4261 ctatcgatta caaaatagat ggattggaat ttatccttga tataaggggt atccaatatc
     4321 ttttctacat tgaaatcaac acgtttcgat ggaatgcctg atttgaggca ataaatactt
     4381 tcaccttgtg aagaaagaat acctgagccg taaatacgca gtaatccatt gtcgcgtatc
     4441 aatccgaatt ctacggtgta ccaataaata cgggagatca attcaactgc aaccggattg
     4501 tcgatatagc tgagcgctat cttactcaat ccatataagt aggcacaata ggttttattg
     4561 gtaagtaaag gcaaatgtgc gaacacatca tggaacatat ccggctcttc gaggtagtcg
     4621 agttgctcca tactgcgcag ccaggtagaa gccgggaagc gacgattctt gagtaattca
     4681 aaaaatggtt tgttatcgat caaacccgga acaaccactg cttcccatcc cgtgagggtt
     4741 tcgagacgaa tattgaattc atcgaatttc gggatcctgt cggccgagaa atgaataagg
     4801 tctattccct gcatgaacac ttcggcagca cgatccttca acaaggccat ttgcctgtcg
     4861 aataatatct cccaaacttt aaagtgttca ggtgtataag cgctgtagtc ttgtgtttgg
     4921 gggattaaga cggtaggctc catttgcatt gtcaggctgt tgagaagtta gaacgctaat
     4981 gtaacccatt ttcgctcccc gatggtcatt tttgggtgaa attgtgttaa catgtaattc
     5041 acagagcgaa aatgcagaac tgttgagata ttggggtttg tgagtgggca tacctataaa
     5101 aggccctctc gataatcgaa agggcctttt agtgtcagct aagtaaaatg taacgggtta
     5161 tagtagctat tttattgaag gatgatcttc tgtgtttcaa taatattgcc agtagtatct
     5221 gaaaaaatgg ccatatatac accttttgga taattagcaa tatccagtgt cacactgcct
     5281 tccataacac cttcactcat ttgttcaccg gataaggaaa aaatcctata gttcgctgac
     5341 aagccattca atgagccaag ggtaacgatt gttctagcag ggttagggaa aatgaggaat
     5401 tgcgtgccat aatttatatc atacacagct tcacgatcat caatccagaa cggatattgg
     5461 caatacacgg tagcaagaat atccgatatt tctttcagag taatcgagga gatatataac
     5521 tgtttgcata ctccatcagg taaaccgttt gtatactcag attccacacg attcctgagc
     5581 caattgaatt tctggtatgc atagttgtaa tctcctgcag tgaaaagtaa gtaaccatca
     5641 cgatattgat tggcaataga aattcttcca
//
LOCUS       DAOQOU010000190         5653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8621_length_5653_cov_5.134334, whole genome shotgun sequence.
ACCESSION   DAOQOU010000190 DAOQOU010000000
VERSION     DAOQOU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8621_length_5653_cov_5.134334"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /gene="lysS"
                     /locus_tag="PLM90_12770"
     CDS             complement(<1..1487)
                     /gene="lysS"
                     /locus_tag="PLM90_12770"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMZ90302.1"
                     /translation="MSQHLSEQEQIRRESLIAIRNMGIDPYPAAAFPITHNSSEILKG
                     FPTDPESYKEVSFAGRIMSRRIMGKASFAVLQDSAGKIQVYMKGEEICPPGDMSLYDE
                     LFKKHLDIGDIIGVTGYVFTTKTGETSIHVKTLTLLSKSLRPLPIVKTDADGNVFDAF
                     TDPEQRYRQRYLDLIVNPHVRDVFVKRSKLVNTMRSFMNQFGFLEVETPILQPLYGGA
                     AARPFKTHHNALDMTLYMRIANELYLKRLIVGGYDGVYEFSKDFRNEGMDRFHNPEFT
                     QVELYVAYKDYQWMMDFVEEMIEKVAMEVTGSTEVQVGEHAISFKRPWKRFTMFEAIE
                     HFTGIDISAMDEQQLFKTARELKVHVDGTMGKGKIIDEIFGEKCEPYLIQPTFITDYP
                     VEMSPLAKRHREKDGLVERFEAICNGKEIMNSFSELNDPIDQRHRFESQLELGKRGDE
                     EAMQLDEDFLRALEFGMPPTAGIGIGIDRLSMIMTNQHAIQDVLL"
     gene            complement(1783..2424)
                     /locus_tag="PLM90_12775"
     CDS             complement(1783..2424)
                     /locus_tag="PLM90_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HMZ90303.1"
                     /translation="MAIRTTFYIDGFNFYNGIKSASKSINKDWKKYYWIDFVKFCQLF
                     LTDSHELVKVKYFSAPPFDSGKQSRQSALFKANKIINGDKLSIINGKYYKKPITCMAS
                     CKETFHISEEKRTDVNISVEMIGDCAFNNTDLIVLISADSDLVPPIEYVIKKFPDKKV
                     KVYFPPKRSSYDLKSMVNNNVVYLDKNEGKFKAAIMPDLVSNGVKHVNKPELW"
     gene            2738..4177
                     /locus_tag="PLM90_12780"
     CDS             2738..4177
                     /locus_tag="PLM90_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HMZ90304.1"
                     /translation="MLILSNYIGGTLQSPVNGRYIDNYEPSTGSVYGKIPDSDEHDVN
                     LAVAAAKAAFPAWAAMDPASRSRQIHRIAEGINNRFEELAAAESRDSGKPVGLARRMD
                     ITRARDNFEFFAAAALQFASESHNQPGFINYTLRQPIGVVGCISPWNLPLYLFTWKIA
                     PALAAGNCVVAKPSEVTPVTAHMLAEICMEVGLPAGVLNIVHGAGPQCGASIVKHPEI
                     KGVSFTGSTRAGREIASVAAPMFKKLSLELGGKNPVIIFADSDYEEMLNTTVRSSFTN
                     QGQICLCGSRIYVERPLYNRFRSDFVSRVQQLKVGDPAAADTNIGAVVSETHYNKILS
                     YIALAKEEGGTILCGGEAVKMEGEQAGGWYIAPTVIDGLSPQCRTNQEEIFGPVVSIQ
                     PFDTEEEAVTLANNTDYGLAAVIWTNHLSRAHRLGDRLASGIVWINCWLERDLRTPFG
                     GVKNSGVGREGGWEALRFFTEAKNICVKI"
     gene            4190..5293
                     /locus_tag="PLM90_12785"
     CDS             4190..5293
                     /locus_tag="PLM90_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ90305.1"
                     /translation="MVLLNRNINPTTGLLAGLTALVMSSCGTYTSAIRTEQPAYTQEQ
                     PQAEETAFIEDFWTDSARTLIAPRLENPVLAETGLRSVEHNSAAIQLPMSEELAQLGE
                     NARLQDEALGDNIGGIDFANPEEAEHFEMYDELFLANELNAGEVFDPSKLTDSIPVYL
                     VSPWSNCYTFPLRQSKVNSDFGWRHSRFHSGIDLDLEVGDDVYASFDGIVKKSDYVSG
                     YGNLVVVKHFNGLETYYAHLSKILVAEGDTVNAGDKIALGGNTGRSTGPHLHYEIRYR
                     GAAINPKYLVDFKNEVLVSESFYLKREHFKPTTESSSSATSTTGKKYVTVKSGDTLSK
                     IAQRNHTTVSKICKLNNISAKKILRPGQRLRVR"
     gene            complement(5474..>5653)
                     /locus_tag="PLM90_12790"
     CDS             complement(5474..>5653)
                     /locus_tag="PLM90_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90306.1"
                     /translation="VDDYTVEIFDINGKILDQKKFHDNNCPIYTDTYPTGVYIVRLTN
                     MTEKTSETVKLVVKR"
BASE COUNT         1561 a         1185 c         1246 g         1661 t
ORIGIN      
        1 aacagcaaca catcctgtat cgcatgctga ttcgtcatga tcatcgacaa acggtcaata
       61 ccaattccaa tacctgccgt tggtggcata ccgaattcga gggcgcgcag aaaatcttca
      121 tccaattgca tcgcttcctc gtctccgcgt ttgccgagtt ccagttgcga ctcgaagcga
      181 tgacgctgat caataggatc atttaattca gagaatgaat tcatgatttc cttgccattg
      241 caaattgctt caaaacgttc aacaagtccg tctttttcgc ggtgacgttt agcgagcgga
      301 ctcatttcta caggataatc ggtgatgaat gttggttgta tgagataagg ttcacatttt
      361 tcaccgaata tttcatcaat gatttttcct tttcccatgg tgccatctac atgcactttt
      421 aattcacgcg cagttttaaa taattgctgt tcatccattg cggaaatatc aattcccgta
      481 aagtgttcaa tagcttcaaa catggtgaaa cgcttccatg ggcgtttgaa tgaaatggca
      541 tgttcgccaa cctgaacttc tgttgaaccg gtaacttcca ttgccacttt ttctatcatc
      601 tcttccacaa aatccatcat ccattgatag tctttgtagg caacatataa ttcaacttgg
      661 gtaaattccg gattgtgaaa acggtccatg ccttcattgc ggaaatcttt ggagaattca
      721 tacacgccat cataaccgcc aacgatcaaa cgttttaaat ataattcatt ggcaatgcgc
      781 atatacaatg tcatgtccaa tgcattgtga tgcgttttga atggacgtgc agcagcgcca
      841 ccgtagagtg gttgtaaaat tggagtctcc acttcaagaa aaccgaattg attcataaaa
      901 ctgcgcatgg tattcaccaa tttgctgcgt tttacaaata catcgcggac atgtggattt
      961 acaataagat cgagatagcg ctggcgataa cgttgttccg gatcagtgaa tgcatcgaat
     1021 acatttccat cggcatcggt tttaacgata gggagtggac gcaagctttt gctcaacaag
     1081 gtcagcgttt taacgtgaat ggaagtttcg cctgttttgg tggtgaaaac atatccggtt
     1141 acgccaatga tatcgccaat gtcgaggtgt tttttaaata gctcgtcgta taagctcata
     1201 tcgccgggag ggcaaatttc ttcacccttc atatacactt gaatttttcc tgccgagtct
     1261 tgcaatacgg caaaggatgc cttccccata atgcgacggc tcataatgcg gcctgcaaag
     1321 cttacttcct tatacgattc cggatcggtt gggaatccct ttaaaatctc tgacgaatta
     1381 tgtgttattg gaaatgcagc tgcgggatat ggatcaatgc ccatattcct gatagcgatc
     1441 aaactttctc tgcgtatctg ttcttgttct gaaaggtgtt gcgacatatg gtgcaaaggt
     1501 agaaaaggaa aagcggttat gggaaaaatt atttagttta ttcctactgt atacaggact
     1561 ccgctttgaa atgcctttac taatttgctt gtgtctttgc tttgtatgca ctaatgcaat
     1621 agacacctgg tttgggagta ttttgaaggt ccggtatgat ttgctttaag tccacttttt
     1681 tgtcaaatat ccaaagccct ttacgagtac atttttttaa gctgagtcct gatatatttg
     1741 tggaattgga atagcttctg ccgcatagag taacgcaaaa aattaccaga gttcaggctt
     1801 gttgacatgc tttactccgt ttgaaactaa atcaggcata attgctgctt tgaatttccc
     1861 ttcgtttttg tctaaataaa caacgttgtt attcaccatt gattttaaat cataagagct
     1921 tcttttaggt gggaaatata ctttcacctt tttgtcggga aattttttta ttacatattc
     1981 aattggcgga accagatcgc tgtctgcaga tattagtaca attaaatcag tattattaaa
     2041 tgcgcaatca ccaatcattt caactgaaat attcacatca gttctttttt cttctgagat
     2101 atgaaatgtc tctttgcagg aggccatgca ggttatcggc tttttgtagt attttccatt
     2161 gataatacta agcttgtccc cgttgattat cttatttgct ttaaataaag ccgattgcct
     2221 actttgcttg cctgaatcaa aaggcggggc gctaaagtat tttaccttta caagttcatg
     2281 agaatcagtt aaaaatagtt ggcaaaattt tacaaaatca atccaataat attttttcca
     2341 atccttatta atagattttg acgcagattt aattccgtta taaaaattga aaccgtcaat
     2401 ataaaaagta gttctaattg ccatataaaa aaaaaccgcc agtatgtcgg cggtataact
     2461 tatccagtat agataagcga cgttggtaca aagttattgc aaaaatttat ttccgagcaa
     2521 ataattttta gtaggacaaa tatgttgttt taatgagttg tttactaaaa tatacaacat
     2581 tcatgccgta tttaaatctg aatggaaatg cctgtcctcg ggatttgtca gaacgcacct
     2641 atatattgaa acctcaggat acgcgctgca atggaatttt gcctgaattt tagtatcttc
     2701 gccgttcgaa acaattgtaa acctttacgg atacgttttg ctcatattat caaattatat
     2761 cggaggcacg ctgcaatccc ctgtaaatgg tcgttatatc gataactacg aaccatcaac
     2821 aggctccgta tatggcaaaa tacccgattc cgacgaacat gatgtaaacc tggcggtagc
     2881 tgcagcaaaa gcggcttttc cggcatgggc tgccatggat ccggcctcgc gcagtcgaca
     2941 gattcaccgc atcgccgaag gcatcaacaa ccgcttcgag gagcttgcgg cggcagaatc
     3001 gcgtgattca ggtaaacctg ttggtctggc cagaaggatg gatatcaccc gtgcgcgcga
     3061 caattttgag ttttttgctg cggcggcgtt gcaatttgct tctgaaagtc ataatcaacc
     3121 cggtttcatt aactatacgc tgcgtcagcc aattggcgtg gtagggtgca tttcgccctg
     3181 gaacctgccc ctgtatctgt ttacatggaa aatagctcct gcgctggctg caggcaattg
     3241 cgtggtggca aagccttcag aggtgacacc tgtaacggcc catatgctgg cagagatatg
     3301 tatggaagtc ggtttaccgg caggggtgtt aaatattgtt cacggcgcgg gtccgcaatg
     3361 tggggcatcg atagtgaaac accctgaaat taagggagtt agcttcaccg gcagcacccg
     3421 ggcgggtaga gagatagcct ccgttgccgc cccgatgttc aaaaagctga gtttagagtt
     3481 ggggggtaag aatccggtta ttatctttgc agacagcgat tacgaagaga tgctgaacac
     3541 aacagtgcgc agcagcttca ccaatcaggg gcaaatttgt ctctgcggaa gtagaattta
     3601 tgtggaacgt cccctctaca accgtttccg ttctgatttc gtaagccgcg ttcagcagct
     3661 caaagttggc gaccccgcag cagcggatac caatattggg gccgttgttt cggaaacaca
     3721 ttacaataaa atactgtcct atatcgcctt agcgaaggaa gagggcggaa ccatcctatg
     3781 tggtggcgaa gcggtgaaaa tggaaggtga gcaggcagga ggctggtata ttgccccgac
     3841 agtaatagat gggctgagtc cacaatgcag gacaaatcag gaggagatct tcggtccggt
     3901 tgtgagtatt caacctttcg ataccgagga ggaggcggta actttggcca ataatacgga
     3961 ttacggtttg gcagctgtta tctggactaa tcacctctcg cgggcacacc gccttggcga
     4021 tcgattggca tccggcattg tctggatcaa ttgctggctg gagcgcgacc tgcggacacc
     4081 ttttggcggc gttaaaaata gcggcgttgg aagagaaggg ggatgggagg ctttacgctt
     4141 ctttacggaa gcaaaaaata tttgtgttaa aatctaaaat tggaggacag tggtattgct
     4201 aaatcgaaac atcaacccaa ccacaggttt actcgcagga ctcaccgcgt tggtgatgag
     4261 ttcatgtggg acatacacat ctgccatccg gactgaacag cccgcttata ctcaggagca
     4321 accgcaagct gaggaaacgg cgttcatcga agatttctgg acggattccg cacgcacttt
     4381 gatagctccg cgattggaga atcccgtgct cgcagaaacc ggactgcgca gcgttgaaca
     4441 taacagcgct gccattcaat taccgatgag cgaagaactg gcacaactcg gagaaaatgc
     4501 acgtctgcaa gatgaggcat tgggagataa tatcggtggt atcgacttcg ccaacccgga
     4561 agaagccgaa cattttgaaa tgtatgatga attatttctg gcgaatgaac tgaacgccgg
     4621 tgaagttttc gacccgtcga aactgaccga tagcattcct gtttatttgg ttagtccatg
     4681 gagtaattgc tatactttcc cgctgcgtca aagtaaggtg aattccgatt tcggatggcg
     4741 tcatagccga ttccactcag gcatcgactt ggatctggaa gttggtgatg atgtttatgc
     4801 ttcatttgac ggcattgtga aaaaatctga ttatgtatca ggttatggta acctagttgt
     4861 ggtgaaacat ttcaacggac tcgaaacata ttacgctcac ttgtcgaaaa ttcttgttgc
     4921 cgaaggcgat accgtaaacg ccggcgataa aattgcattg ggtggtaata caggtcgaag
     4981 caccggtccg cacttacatt atgaaattcg ttatcgcgga gcagccatca atcctaaata
     5041 tctggtagat tttaaaaatg aagtgcttgt ttctgagtcg ttttatttga aacgcgagca
     5101 ttttaaacct accactgagt cgtcatcaag cgcaacttca actacaggta aaaaatatgt
     5161 taccgtgaag tcgggcgata cactttctaa aattgcacag cgaaatcata ctactgtaag
     5221 taagatatgt aagctgaata atatttccgc caaaaaaata ttgcgacccg gacaacgatt
     5281 gcgggttaga taatctttca taaaaatgcc gacagttatt gccggctttt tttatgctat
     5341 tcaaattgtt taattatagc accctaatac acatcgtgta tgctacacat accatcttcg
     5401 cgccttcgcc cctttgcccc ttagcgcgaa aaaagcagat caatatggtt tctgttttct
     5461 gattttaaca ggattatctt ttaaccacca atttcaccgt ttcagaagtt ttttctgtca
     5521 tatttgtcag gcgaacaata tatactcctg taggataagt gtcggtataa atcgggcaat
     5581 tattatcatg gaatttcttt tgatcgagaa tttttccgtt gatgtcaaat atttctaccg
     5641 tataatcatc gac
//
LOCUS       DAOQOU010000191         5627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8678_length_5627_cov_5.595477, whole genome shotgun sequence.
ACCESSION   DAOQOU010000191 DAOQOU010000000
VERSION     DAOQOU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8678_length_5627_cov_5.595477"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..798)
                     /locus_tag="PLM90_12795"
     CDS             complement(133..798)
                     /locus_tag="PLM90_12795"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HMZ90307.1"
                     /translation="MPYNTQLPQMILREYGRHIQNLVNFACKIEDDEVRNKLVQDIIE
                     LMGTMNPTLRNVEDFRHKLWDHIYMMSDFKLKANSPYPVPERETLTRKSIKLSYPKTK
                     IRFAHYGKNVETCVNKAVEMTDPELRQEYAQVIANYMKLVYQNWNSDNVNDNIIKQEL
                     ESLSHGAIKLEDDTDLDTLTRQNRGPQQGQQQRQQQHRHQGNNRNFKRNNGGNHKNFK
                     KNR"
     gene            complement(908..1411)
                     /locus_tag="PLM90_12800"
     CDS             complement(908..1411)
                     /locus_tag="PLM90_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90308.1"
                     /translation="MKNYLIALLLCITGVAKAQGPPPPPPGMDDEKIEALRIAFLTKY
                     LDLSTDEAQKFWPVYNDMQEEMDVIKEKEKDLRKGKEIDKMTDEELNKMISSHFENEQ
                     KMLDIKKKYLEEFKKVLPLKKVALLADAENEFKREMMKHARDKQGPPPPGGGKPGGQQ
                     PTPDADH"
     gene            complement(1452..2051)
                     /locus_tag="PLM90_12805"
     CDS             complement(1452..2051)
                     /locus_tag="PLM90_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90309.1"
                     /translation="METEQKNTLQELEDAGSTLPELLRRNTMRVPEGYFEGLEDRLIN
                     QVIAAEAPKSQTGKSLRMWMYISAAASVLIVAGIVFLQRNAANHSLEMQFAKMSDAEL
                     DNYINEQIASLSSDDIYNYLSEEIHSIEASDILSTELVGATDADEHFQEAMHEQVYPS
                     TENGTQGPAILDEELLEDIDDVLLQEYLNNAAIFEDMAL"
     gene            complement(2081..2632)
                     /locus_tag="PLM90_12810"
     CDS             complement(2081..2632)
                     /locus_tag="PLM90_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ90310.1"
                     /translation="MSDQEIVQLCLDPLHRERGYRLLVSTYSKKIYWHVRRMVTDHDD
                     ANDVVQNIFIKVWKGLPEFRADSKLYTWLFRIATNESISFINEKYRRSSVALDHEQAS
                     HIQRLTQDAWFDGDDTQKQLQHAIDSLPDKQKQVFIMRYYDEMKYEDMEAILGTSTGA
                     LKASFHHAVKKVEQFLKAGLNRG"
     gene            complement(2696..3655)
                     /locus_tag="PLM90_12815"
     CDS             complement(2696..3655)
                     /locus_tag="PLM90_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ90311.1"
                     /translation="MITIDYTNQKDTRSGFGAGILEAGRNNPNVVALCADLVGSLKLE
                     AFIKEFPERFVQCGIAEANMTGIAAGMTIGGKVPFACTFANFATGRGYDQIRQSIAYS
                     NKNVKIAASHAGLTLGEDGATHQICEDIGLMQMLPNFVVINPCDYNQTKAATIAAANY
                     EGPVYLRFGRPKWPIFYPADMPFEIGKAWKIQDGSDVSIFATGHLVLKAIEAAVILVE
                     KGYSVDLINIHTIKPIDAEAVIQSVSKTKCAVTAEEHQVIGGLGSAVGKVIAEHMPVP
                     IEFVGVQDTFGESGTPDQLLEKYGMSAAHIVEAALKAIGRKSA"
     gene            complement(3704..4150)
                     /locus_tag="PLM90_12820"
     CDS             complement(3704..4150)
                     /locus_tag="PLM90_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90312.1"
                     /translation="MRVDVRGETYKGKINCIIKKNGDIINAQQTEYAQKMLTGDYDVE
                     ILTIPVIYINHAQVEQDKTTTLEIAAPGFVTFSKNEALIGGIYEYRDNKLTEIYELNE
                     ASLKEAVAIQPGKYKVIYRYASKKRMDATITVEFEVVSGTSLTVRL"
     gene            complement(4135..5073)
                     /locus_tag="PLM90_12825"
     CDS             complement(4135..5073)
                     /locus_tag="PLM90_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ90313.1"
                     /translation="MQKVITYRLKMMLLCILIATSSSLFSQGQTTRILIILDASRSMG
                     ENFNRTSKMQAARDIVGHIVDSLNGNPNIQLAMRVYGHQSPQPLNDCEDSKLEVAFRT
                     KNAATIKSRMETIRPQGVTPIAYSIEQSIADFGQDAKNYRNILILVSDGFESCGRNPC
                     EVVQRMRQQGILTKSYVIGIGIDALKYSEFSCMGEFTNISTEDQTEDVANSVIAKIFN
                     SVFVRVDLLDTENRPEETDAVMTFYDRSNHLPKFNYYHTINPKGNPDTITLDPAVNYD
                     MQVHTVPETWKRDVNLTSGKLMSSPNRPRRDICVLM"
     gene            complement(5075..>5627)
                     /locus_tag="PLM90_12830"
     CDS             complement(5075..>5627)
                     /locus_tag="PLM90_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90314.1"
                     /translation="DITVHTTPPIFLNDVELTPGKHNIIGIDVPLGTLVLSEGGNTTL
                     SEKQLVLRDNETGVIIYVQNFNTKMKYVAGTYDIDILTLPRLHYDNYEIKGGVESKID
                     IPVSGTLNITLTANTTYSIYTVKNDQLEKVYENTMSAGMDPVQLLPGEYIVVSRSNIK
                     KVAASTKEQRVQIKSAKTTTLKL"
BASE COUNT         1459 a         1286 c         1089 g         1793 t
ORIGIN      
        1 gaatattcct gataattacc tgcttatcgg taagcaatac ggcacatatc acttgcagtg
       61 cctcattctt tgccccctga ggaatcaatt caccctttag gcgccttcct ccggtgatct
      121 caaatgcgtt catcaacgat tctttttgaa attcttgtgg ttgccgccat tattccgctt
      181 aaagttgcgg ttattgccct gatgacgatg ttgctgttgg cgctgttgtt gtccttgttg
      241 cggtccgcga ttctgacgtg tcaatgtatc cagatcagta tcgtcttcca atttaatagc
      301 accatgactt aaactctcca attcctgttt gatgatattg tcattcacat tatcactgtt
      361 ccagttttgg taaacgagtt tcatataatt ggcaattacc tgagcatatt cctgacgcaa
      421 ttcaggatct gtcatctcaa cagctttatt cacacaggtc tctacatttt ttccgtaatg
      481 tgcgaagcga atctttgttt tcggataact taacttaata ctttttctcg tcaacgtttc
      541 gcgttcagga acagggtaag gggaatttgc tttcagtttg aaatcactca tcatataaat
      601 atgatcccat aatttatgac ggaagtcttc aacgttcctc aaagtgggat tcatagttcc
      661 catcagttca attatatcct gcacaagttt attgcgcact tcatcatctt caatcttgca
      721 ggcaaagttg accaggtttt ggatatggcg accgtattcg cgcaatatca tctggggcaa
      781 ctgggtatta tatggcattg aattgtattt gcaacaaagg tagcagattg caccacagta
      841 agcaagtgcc caaaacactg cgggcaccct tctcagagcg cccgcagctt gtacttcaaa
      901 ttaggggtta atggtcggca tccggagtcg gttgctgtcc accgggcttg ccgcctcccg
      961 gaggaggagg tccctgcttg tcgcgggcgt gtttcatcat ctcacgctta aattcgttct
     1021 cggcatctgc cagcaaggca acctttttta aaggcaatac ctttttgaat tcctcgagat
     1081 acttcttctt gatatcaagc atcttctgct cattttcaaa atgactgctg atcattttat
     1141 tcagctcttc atcggtcatt ttatcaattt ctttgccctt gcgaagatct ttttcctttt
     1201 cttttattac atccatctct tcctgcatat cattatatac cggccagaat ttttgtgctt
     1261 catcagtgct gaggtcgaga tattttgtca gaaaagcaat gcgcaaagct tcaatttttt
     1321 catcgtccat acccggaggt ggtggtggcg gaccttgagc tttagccact cctgttatac
     1381 acaagagtaa agcgatcaaa taatttttca taatggtcaa tttatggttt gaggttaggt
     1441 aacatttcga attataaagc catgtcttca aaaatggctg cattatttaa atattcttgt
     1501 aataacacat catctatatc ctccaacaat tcttcatcca aaattgcggg accttgcgtt
     1561 ccattttctg ttgaaggata tacttgctca tgcatagcct cctgaaaatg ttcatcggca
     1621 tctgttgcac caactaattc tgtcgacaaa atatcagaag cttctatact atgaatttct
     1681 tcactcaaat aattataaat atcatcacta ctcaacgatg caatttgttc attgatataa
     1741 ttatccaatt cagcatcact cattttcgcg aattgcattt ccagtgaatg attagcggca
     1801 ttgcgctgta aaaaaacaat tccggcaaca atgagtactg acgcagcagc agatatatac
     1861 atccacattc gcagcgattt ccccgtttgc gacttcggag cctcagcagc tatcacctga
     1921 ttgatcagcc tgtcttccag cccttcaaaa tacccttccg gcacacgcat ggtattgcgc
     1981 ctgagcagtt cagggagggt gcttccggca tcttccagtt cctgcagtgt atttttttgt
     2041 tccgtttcca tactgttaat atttgattag atatatataa tcacccacgg tttaatccgg
     2101 ccttcaaaaa ttgttcaact tttttcacgg catgatgaaa actggctttc aaagcacctg
     2161 ttgaagtgcc caaaatcgct tccatatcct catatttcat ttcatcataa taacgcataa
     2221 taaacacttg cttttgcttg tcaggcaaac tgtcgatggc atgctgcaac tgcttctgtg
     2281 tatcatcacc atcgaaccag gcatcttgtg tcaatcgttg aatatgcgac gcttgttcgt
     2341 gatccagcgc tacactgctg cggcgatatt tttcattgat aaaagatata gactcattgg
     2401 tggcaatgcg aaacagccac gtatacaatt tgctgtcggc acgaaattcg ggaagtcctt
     2461 tccacacttt aatgaaaata ttctgcacaa catcattcgc atcgtcatga tcagttacca
     2521 tgcgacggac atgccaataa attttttttg aataggtgga taccaacaat cgataaccac
     2581 gctcgcgatg gagtgggtca aggcataatt gtacgatctc ctggtctgac atccgcggta
     2641 aattggtgtt tggtcagcac cctaccgatc gcaatcggaa aatttcgggt tgaagttaag
     2701 ccgactttct gcctatggct ttcaatgcag cttctactat gtgagcagca ctcatgccat
     2761 acttttcgag caactgatca ggtgttccgc tttcgccgaa ggtatcctga acgccaacaa
     2821 attcaatcgg cacgggcata tgttcagcaa ttacttttcc aacggcactt cccaatccgc
     2881 ctatcacctg atgttcttcg gcagttactg cgcactttgt tttgctaacc gattgtatga
     2941 ctgcttcggc atcgattggt ttaatggtat gaatattaat caggtcaaca ctatatcctt
     3001 tctcaacaag gataacagct gcttcaatgg cttttaatac gaggtgtccg gttgcgaata
     3061 tcgacacgtc actgccatcc tgtatcttcc aggccttacc aatctcgaaa ggcatatctg
     3121 cgggatagaa aatcggccat ttcgggcgac cgaaacgcag gtaaaccgga ccttcatagt
     3181 tggcagcggc aatggtggca gccttagttt ggttgtaatc gcatgggttg atcaccacga
     3241 aattcggcag catttgcatc aaacctatgt cttcgcagat ctggtgggtt gcaccatctt
     3301 caccaagtgt aagtccggcg tgcgacgcag ctattttcac atttttgttt gaataggcaa
     3361 tagactgacg aatctggtca tatccccttc ctgttgcgaa atttgcaaat gtgcaggcaa
     3421 aaggcacttt cccgccaatg gtcataccgg ctgctattcc ggtcatattc gcctcggcaa
     3481 taccacactg cacaaagcgt tcagggaatt ctttaataaa agcttccagt ttcaatgagc
     3541 caaccaggtc ggcacatagc gctaccacgt ttgggttatt acgtcctgcc tctaaaatgc
     3601 cggcaccaaa gccggagcgg gtatctttct gattggtata atcaatggtg atcacgtctg
     3661 cgaattttca attaattgtt tgcagcgcga agataaggat aagttacagt ctgacggtaa
     3721 gtgaagtgcc tgataccacc tcaaattcca cagtaatggt agcatccata cgtttttttg
     3781 atgcatagcg gtagatgacc ttgtatttcc cgggctgaat ggcaacggct tcttttaagg
     3841 aagcttcatt caattcatag atctcggtga gtttattatc gcgatactca taaatacccc
     3901 cgattaatgc ttcattttta ctgaatgtca caaatccggg agctgcaatt tcaagcgttg
     3961 ttgttttatc ctgttcaacc tgcgcatgat tgatgtatat aacaggaata gttaaaattt
     4021 caacatcata atcgccggtg agcatttttt gcgcatattc agtttgctgc gcattaatga
     4081 tatcgccatt tttcttgatg atgcagttga tcttcccctt ataggtctca cctcttacat
     4141 caacacgcaa atatccctgc ggggccggtt gggtgatgac attaattttc ctgatgtcaa
     4201 attcacatcg cgcttccagg tttcaggaac ggtatgtacc tgcatgtcgt aattaactgc
     4261 cgggtcgagt gttatggtgt caggatttcc cttcggattg atggtatgat aataattgaa
     4321 tttcggaaga tgattacttc tgtcataaaa ggtcattacc gcatccgttt cttcaggcct
     4381 gttttccgta tcgagcagat ctacccgcac aaacacagaa ttaaatattt tggcgataac
     4441 actatttgcc acatcctcag tctgatcttc tgttgaaata ttggtgaatt cacccataca
     4501 agaaaactca gaatatttta atgcgtcaat tcctattccg ataacatacg atttggtgag
     4561 tataccttgc tgacgcatgc gttgaaccac ttcacacggg tttcgaccgc aggattcaaa
     4621 tccgtcgctc accagaatta agatatttct ataattcttt gcatcctgtc caaagtcggc
     4681 aatagattgt tctatagaat aagcaatagg cgtaactccc tgcggcctga tggtctccat
     4741 gcgcgattta attgttgcag cattttttgt gcgaaaggct acctccaatt tcgaatcttc
     4801 gcaatcgttc aatggttgcg gactctgatg accatatacg cgcatagcca gctgaatatt
     4861 tgggttgccg tttaaactat ctacaatatg cccgacaata tcacgtgcag cctgcatttt
     4921 tgaggtgcga ttgaaattct cgcccatact gcgactggca tccaaaataa tcagtatgcg
     4981 cgtagtttga ccttgagaaa ataatgatga agaggttgca atcagtatac acaacaacat
     5041 catcttcagg cgatatgtaa ttactttttg catatcagag ttttaatgtt gttgtttttg
     5101 ccgatttaat ttgcacacgt tgttccttgg ttgaagcagc tactttctta atattagagc
     5161 gactcaccac aatatattcg cccggtaata attgcaccgg atccatccct gccgacatgg
     5221 tattttcata cactttttcc aactgatcat tttttacagt atatatactg tatgttgtat
     5281 ttgctgtaag tgtaatattt aatgttcctg atacaggtat atcaattttg ctttcaacac
     5341 ctcctttgat ctcataattg tcataatgca aacgcggtag tgtaagtatg tcgatgtcat
     5401 aagtgccggc gacatatttc attttggtat taaaattctg cacgtaaata atcacaccgg
     5461 tttcattatc gcgcagcacc aattgcttct cacttaatgt tgtatttcca ccttcactta
     5521 acaccaaagt ccctaatggc acatcaattc cgataatatt atgtttaccg ggcgtcaatt
     5581 ccacatcatt caaaaatatt ggtggtgtag tatgtacggt aatgtca
//
LOCUS       DAOQOU010000192         5612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8709_length_5612_cov_5.862336, whole genome shotgun sequence.
ACCESSION   DAOQOU010000192 DAOQOU010000000
VERSION     DAOQOU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8709_length_5612_cov_5.862336"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="PLM90_12835"
     CDS             <1..1778
                     /locus_tag="PLM90_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HMZ90315.1"
                     /translation="SPLTNSPTHPIWLKSKNLYFALSFNSVQRKSMTQKTESWGSRVG
                     LILAMAGNAVGLGNFLRFPVQAIQNGGGAFIIPYLVCFLLMGIPLLWIEWATGRFGGS
                     KGHHSTPFILDSMDKRKFWKYIGVFGIFTNLAVAAYYCYLESWTLNYVWNSISGTFRG
                     ESAESVSAYFNHYVNAGDAHQHWTSWIADNPAPIIFWIICLLLNTWILSKGLSGGVEK
                     VAKIGMPLLIVFGVFLAIMAVSLRAGQHGAINGGEVGLNFLWTPNFDTIWNPKVWLAA
                     AGQIFFTLSVGMGSIQCYASYVRKKDDIALNAMSAGWMNEFVEVVLGAAIIIPISVGY
                     LGIDKVVEMTQSGGLGLGFRTLPYLFEQWGPTLAVICGVFWFGLLFFAGITSSLAMGT
                     PWMGFMTDEFGWSRQKGAWSFGALVLILGLPTVLFFNQGVFDEYDYWAGTVSLVVFAL
                     GETILFAWVFGMTKGWQEINSGGDIRVPSVYKFVIKWVTPLLLLFVFLGALITPQGND
                     WVNAFNNGWVLDNSSIIKQITNAGLKEQMHAATTAGDTATMDALHDKLLYINIARILL
                     VSVFLGVAWLVYTAYRKRIKEGRFI"
     gene            1775..1918
                     /locus_tag="PLM90_12840"
     CDS             1775..1918
                     /locus_tag="PLM90_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90316.1"
                     /translation="MSSSALIMMIATVSVVTLVTAYFFYLVLKTPPREEPDSFSENDD
                     VKR"
     gene            2072..2938
                     /locus_tag="PLM90_12845"
     CDS             2072..2938
                     /locus_tag="PLM90_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMZ90317.1"
                     /translation="MLLILPDILHALIKKPDTKDNYALWQSLKMRTDTTSIYQSDHSF
                     QDSNSYAENYNYPFQRYKTEQQNVELSFITPSKPFNPNMISAEEWQSFGVPKYVAERI
                     IKYRQKSGTFKSSTDVKKIYGFPDDVFATIEPFMIFQDSSAVTVADHSGGDLQIVLTS
                     TNLNTCTKEDLLSLGFSPNDATRILRFREQAGGIYDSQQLFGVYGIDADKLQELIKWL
                     EVDKNSVIKINLNTIDSTTLANHIYISDILAGSIIRYREATGKFYSVNELLKVKGMYP
                     ALFEKLKPYLQL"
     gene            2935..3465
                     /locus_tag="PLM90_12850"
     CDS             2935..3465
                     /locus_tag="PLM90_12850"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HMZ90318.1"
                     /translation="MSIEQELKQAIRDVADFPKEGIVFKDITPLLSDPVLSDKIGDAL
                     LDRVKDLKPDAIVAIESRGFLYGFLLANKLQIPFIPVRKKGKLPGDTLEYTYDLEYGS
                     ATVEIHTMDVKPGWRVLVHDDLLATGGTAQAAAELILMQGAHVAGFAFVVELSFLGGV
                     KKLNHYTDNIVSLATY"
     gene            3501..4640
                     /locus_tag="PLM90_12855"
     CDS             3501..4640
                     /locus_tag="PLM90_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ90319.1"
                     /translation="MSHTISHDISIITDTAKEFAEKYVRPHVMDWDESQHFPIEIFHK
                     AGELGLMGVLVPEQYGGSGLGYIEYMRIIEEIAAVCGSIGLSVAAHNSLCTGHILQFG
                     NESQKSKYLPLLASGQWIGAWGLTEPNTGSDAGNMQCVATREGDEWVLNGTKCWITHG
                     KSSSVMVVIARTGAPRTPNNCTAFVVERGTPGFSAGKKENKLGMRASETTEVIFDGCR
                     ISDAQRLGEVGEGFKQAMKVLDGGRISIAALSLGIAKGAYRAALKYAKERHQFDQPIA
                     NFQAISFKLADMATRIEAADLLTLKAADLKNKGEKVTEISAKAKYYASEVSVAVATDA
                     VQIFGGYGYTKDFPVEKYYRDSKLCTIGEGTSEVQKLVIAREILR"
     gene            4698..4895
                     /gene="rpsU"
                     /locus_tag="PLM90_12860"
     CDS             4698..4895
                     /gene="rpsU"
                     /locus_tag="PLM90_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HMZ90320.1"
                     /translation="MLIIDAREAESIDKALKTYKKKFEKAGIVKELRRRQSFTKPSIE
                     RRQEIIKARYKQTFVTEANQS"
     gene            4948..>5612
                     /locus_tag="PLM90_12865"
     CDS             4948..>5612
                     /locus_tag="PLM90_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMZ90321.1"
                     /translation="MHLEGFYTYLQYERRYSKHTLAAYRNDISQFHDFLVKEYGSEDP
                     DMLDVHHRQVRAWIIALMEDKTAARSVNRKISALKTYYRYCQQQKAINHNPMAKVAVP
                     KTPKNLPLFVERTGMDNLFHLVEASYPEDTETQRFVKLRDRMIVEMLYATGMRRGELL
                     GLKDMSFDRSNQQVKVLGKGNKERIIPISKDMLSLVKEYQTERDRLYGKGEHFLITGA
                     GKPA"
BASE COUNT         1652 a         1060 c         1297 g         1603 t
ORIGIN      
        1 actcaccact caccaattca cccactcacc caatttggtt gaaaagtaaa aatttgtatt
       61 ttgcgctctc attcaactct gttcaaagaa aaagtatgac gcaaaaaact gaatcctggg
      121 gctcccgagt aggtctgatc ctcgctatgg ctggtaatgc tgttggctta ggtaatttcc
      181 tgcgcttccc cgtacaagcc atccagaatg gtggtggtgc atttatcatt ccatacctgg
      241 tatgttttct gttaatgggt attccattgt tatggataga gtgggcaaca ggtcgttttg
      301 gtggcagcaa aggccatcat tcaaccccat ttattttgga ttcaatggac aaacgcaaat
      361 tttggaagta tatcggcgtt ttcggaattt tcaccaacct ggcagtagca gcatattatt
      421 gttaccttga gtcctggacg ctcaattatg tttggaattc aatttcagga accttcagag
      481 gggagtctgc agaaagtgtc agtgcgtatt ttaaccatta tgtaaatgcc ggtgatgccc
      541 atcaacattg gacctcctgg atagctgata acccggctcc gattattttt tggataattt
      601 gtctgctgtt gaatacttgg atcctttcga aaggattaag cggtggtgtg gaaaaagtag
      661 caaaaattgg aatgccgcta ctcatcgtgt tcggtgtatt cctggccatc atggctgttt
      721 ctttaagagc aggtcagcat ggggcaatta atggaggtga agtagggctt aatttcttgt
      781 ggacaccaaa ttttgacacg atatggaatc ctaaagtctg gttggcagct gccggacaaa
      841 tattctttac cctatctgtg ggaatggggt cgatacaatg ctatgcatct tatgtaagaa
      901 agaaagatga catcgcactt aatgcaatga gtgcaggatg gatgaacgaa tttgttgaag
      961 tagtgctcgg tgctgctatc attattccga tttcagttgg ctacctgggt attgataaag
     1021 tagttgagat gacgcaatcc ggcggtcttg gtctgggttt ccgcaccttg ccttatttgt
     1081 tcgaacaatg gggacctacg cttgcagtaa tttgtggtgt attttggttt ggattattat
     1141 tctttgccgg tatcacctca tcattggcaa tgggaactcc ttggatgggt tttatgacag
     1201 atgaatttgg atggagccga caaaaaggcg catggagctt cggtgctctg gtactcattc
     1261 tgggattgcc aacagtattg tttttcaatc aaggtgtttt tgatgaatac gactattggg
     1321 caggtacggt gagcttagta gtatttgcgc ttggcgaaac aatattattt gcatgggttt
     1381 tcggcatgac aaaaggttgg caggaaatta atagtggtgg cgatatcaga gtgccttctg
     1441 tttacaagtt tgtaataaag tgggttacgc cgttattatt attatttgtt tttctgggtg
     1501 ctttgataac gcctcaggga aacgattggg taaatgcatt taacaatggt tgggtgctgg
     1561 ataatagctc gattataaaa caaattacca atgcaggatt aaaagagcaa atgcatgcgg
     1621 caacaactgc aggggataca gctactatgg atgcattgca tgataaattg ttgtatatta
     1681 atatagcacg tattttactt gtatccgtgt ttttaggtgt agcctggttg gtttatactg
     1741 cttatcgtaa acgcataaaa gaaggtagat tcatatgagt agctcagcat taattatgat
     1801 gattgctacc gtttcggtag tgacacttgt aacagcatat ttcttttatc tggtgctgaa
     1861 aacgccaccg cgtgaagaac cggattcgtt ttctgaaaac gacgatgtga agcgataata
     1921 ttttcgctct cattgtttgt tttatgcatg gttttccaat ctgtagggat aggtgttgtg
     1981 gaggtttact atgcgaaaac aattacagaa ttattttatt ttctctagaa gagaaaggcg
     2041 cgcagcatgg ctattgcttt gccttgtgct catgctattg atattacctg atatcttaca
     2101 tgcattaatt aagaaacctg atacaaagga taactatgca ttgtggcagt cgctgaaaat
     2161 gcgaacagat actacatcga tttaccaaag tgatcattcc tttcaggata gtaatagtta
     2221 tgcagaaaat tataattatc catttcagag gtataaaact gaacaacaaa atgttgaatt
     2281 aagctttata actccatcca aaccctttaa tccgaatatg atttctgcag aagaatggca
     2341 atcgttcgga gtgcctaaat atgttgctga aagaataatt aaatacaggc agaagagcgg
     2401 cacatttaaa agtagtacag atgtgaaaaa aatttatgga ttcccggatg atgtctttgc
     2461 aaccatagag ccgtttatga tatttcagga ttcatctgca gtaacggttg ctgatcattc
     2521 tggaggtgat ttgcaaatag ttttaacatc cacgaattta aacacttgca cgaaagaaga
     2581 tttgctatcc ctgggcttta gtcccaacga tgcaacacgt atcctgcgct ttcgcgagca
     2641 agcaggaggc atttacgatt cgcaacaatt atttggtgta tatggtatcg acgccgacaa
     2701 attgcaggaa ttgataaaat ggctggaggt ggataaaaat tcggtgataa aaatcaatct
     2761 caacacaatt gatagtacaa ctctcgcaaa ccatatctat atttcagaca tcttagcagg
     2821 aagcattatc cgatacaggg aagcaacggg caagttttat tctgtaaacg agctcttgaa
     2881 agtaaaaggc atgtatcctg cgctgtttga aaaattaaaa ccatacctcc agctatgagt
     2941 atcgagcaag aattaaaaca agccatcaga gatgtcgccg attttccgaa agaaggaatt
     3001 gtgtttaaag atattacgcc acttctgtca gatccggtgt tatccgataa aattggcgat
     3061 gccttactcg atcgtgtaaa agatctcaag cccgacgcaa ttgtagccat tgaaagtcga
     3121 ggttttcttt atggattttt actggcaaat aaattgcaaa ttccattcat tcctgtgcga
     3181 aaaaaaggta aacttcccgg agatacctta gaatatacct acgatttgga atacggaagc
     3241 gccaccgttg aaattcacac catggatgtt aaacccggat ggcgtgtatt agtgcatgat
     3301 gatttattag ccaccggcgg caccgcacaa gctgcagcag aactcatttt aatgcaaggt
     3361 gcacatgttg caggatttgc atttgttgtt gaattgtcat ttctcggtgg tgttaagaaa
     3421 ttgaatcact atacagataa tattgtatcg ttagcgacat attaatacct ttgtcgtcgt
     3481 tttaaaaaca cgaaaaggct atgtctcata cgatctctca cgatatttcc attataactg
     3541 atacagcgaa agaatttgct gaaaaatatg tccgtccaca tgttatggat tgggacgaaa
     3601 gtcagcattt ccccatcgaa atttttcata aagccggtga acttggtttg atgggtgtat
     3661 tagttcctga acaatacggt ggatcaggat tgggatatat tgaatatatg cgtatcatcg
     3721 aagagattgc cgcagtatgt ggttcaatcg gcttgagcgt ggctgcgcat aattctcttt
     3781 gtacaggaca tattttacaa ttcggcaacg aatctcaaaa atcgaaatat ttgccattgc
     3841 tcgcttcagg gcagtggatt ggtgcatggg gacttacaga gcctaacacc ggtagtgatg
     3901 ccggaaatat gcaatgtgtg gcaacccggg agggagatga atgggtgttg aacggcacaa
     3961 aatgctggat tacgcatggc aaatcaagca gcgtaatggt agtgattgct cgtaccggtg
     4021 ctcctagaac acctaataat tgtacggctt tcgttgtaga gcgaggaacc cccggatttt
     4081 ctgcaggaaa aaaggaaaat aaactcggca tgcgcgctag cgagaccaca gaagtgatct
     4141 ttgacggctg ccgtatcagc gatgcacagc gattaggtga agtaggagag ggctttaaac
     4201 aggcgatgaa agtgcttgac ggcggaagaa tctccattgc ggcacttagc ctcggtattg
     4261 cgaaaggagc ttatcgggca gcattgaaat acgctaagga acgtcatcag ttcgaccagc
     4321 cgatagccaa tttccaggct atcagcttca aattggcaga tatggctacg cggattgaag
     4381 cagccgatct gttaacattg aaggctgccg accttaaaaa caaaggcgaa aaagtgaccg
     4441 aaatttctgc aaaagcaaaa tactacgcat ctgaagtaag tgtcgctgtt gctactgatg
     4501 cagtgcagat ttttggcgga tatggctata ccaaagactt tccggtagaa aaatattacc
     4561 gcgactcaaa gctttgtacc attggcgaag gcacctcaga ggttcagaaa ttggtaatcg
     4621 cccgcgagat acttcgttga tgatacttgt ttgagaaatt aatttttatt acttttgcac
     4681 tcccaaataa tatcagaatg ctcattatcg acgcaagaga agcagaatca atagacaagg
     4741 ccctcaaaac ctacaaaaag aagtttgaaa aggccggtat agtaaaagaa ttgcgcagac
     4801 gtcaatcttt taccaaaccg tctatcgaac gcagacaaga gatcatcaag gctcgttaca
     4861 aacaaacatt tgtaactgag gctaatcaga gctgatccct ttcggtgcgc cgatagcggt
     4921 gcttagtcat tagcgctgca tgaacatatg cacctggaag gcttttatac ttacttacaa
     4981 tacgaacgac gatacagcaa gcatacgctg gctgcctatc gcaacgatat ttcgcaattc
     5041 cacgattttc tagttaagga atacgggtct gaagatcctg atatgcttga tgtgcatcat
     5101 cgacaggtga gggcatggat cattgctttg atggaagaca aaacggctgc ccgcagtgtc
     5161 aaccgcaaaa tttcagcact taaaacttat taccgctatt gccagcagca aaaggccatc
     5221 aatcacaatc ccatggccaa ggtggcagta ccgaaaacgc cgaaaaacct tcccctgttt
     5281 gttgagcgca cgggtatgga taatcttttt caccttgttg aagcttctta ccccgaggac
     5341 acagaaactc aacgttttgt caaattgcgc gatagaatga tagttgaaat gctgtacgcc
     5401 accgggatgc gaagagggga actattaggg ttgaaagata tgtcgtttga ccggtctaac
     5461 cagcaagtaa aagtgcttgg aaagggcaat aaggagcgca ttataccgat aagtaaggac
     5521 atgttatcat tagtgaaaga atatcagacc gaacgagatc gtctatatgg taaaggggaa
     5581 cattttctga taaccggagc aggaaaaccg gc
//
LOCUS       DAOQOU010000193         5521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_8918_length_5521_cov_5.897731, whole genome shotgun sequence.
ACCESSION   DAOQOU010000193 DAOQOU010000000
VERSION     DAOQOU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8918_length_5521_cov_5.897731"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="PLM90_12870"
     CDS             <1..1520
                     /locus_tag="PLM90_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ90322.1"
                     /translation="MRHYRGNKISMIFQEPMTSLNPVFTCGAQVDEAIKLHQKKNKHE
                     ARALTISLFEKVQLPNPERIYRAYPHELSGGQKQRVMIAMAMSCNPDILIADEPTTAL
                     DVTVQKTILQLMEELKSTIHSSIVFITHDLGVIAEIADRVLVMYKGRIVEEGTVLEIF
                     ESPKHPYTKGLMACRPPLGKRLRKLPVIADFMEESADGELIEKQQSVEQAVKINEVNS
                     TETKERLSKLIVQEPVLKVRNLKTWFPSKKSFLGKVEGWIKAVDDVSFDVYPGETLGL
                     VGESGCGKTTLGRTIIRLVDPISGEVHFDNQNLMTLTHRELKPIRKDIQIIFQDPYSS
                     LNPRMTVGNAVREPMQVHGMYSSDRERKEKVIELLEKTGLKADHYNRYPHEFSGGQRQ
                     RICIARALALNPKFIICDESVSALDVSVQAQVLNLLIQLRNEFNFSYIFISHDLSVVK
                     FMSDRMVVMNKGVIEEMGPADEIYDNPQKAYTQKLIDAIPKGQLSDIKGRLMQLQ"
     gene            1545..3629
                     /locus_tag="PLM90_12875"
     CDS             1545..3629
                     /locus_tag="PLM90_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90323.1"
                     /translation="MTRRPIGFLLVIMALFTIVSCRKDLNTGWDTRYKLPIVTGELDL
                     YDLVPDSLTVIGDDQSVTLVYKTELLDYDLTEEAIEIPDTSVEYFVSLDSLSLEDQDL
                     TIPISLGQIAIDLGFPYGTLIIAANGTEVALDPIYGLSTDPTPVDATTFFETATFQHA
                     YLDISLANGLPVDLDTVVFSLKNSVGGETVVYDTFFTVPAGGVPVVETYDLSGMTVEG
                     NLLGQIAYFSTPGSADPVLIDTSDVLVIDMRAYDMELQEAVAVFPEQNLINNANDVQY
                     DMGGPEFTTMGIEEGYVVIYVENTIGDTIHIVYDIPGAVDALGNHVHIETAVGPDEIV
                     DQAFDIAGYTIDLTGTSGNEVNTFYQEFSARINYTGIPTHISLDDSLRVIYGLENIVP
                     NELRGYLGQYEIAMADTTAGVDLFDQFTGGTIEFGDINIDLEIENGLGAGGVVTINSL
                     GGINSETGEVIMLDCPAVIGTPISVNRAIDNPYVPGYTTITLNSANSNINDLIEMFPD
                     KLFYDVSMSVNPNGNEFNYQDFVISTSKINISFNLSMPLEFMANDLTLESEFPVTLDA
                     DAGTDGIGDIDLTLYADNTFPLNASVSIVFYDDFGVALDTLNFGGYNIKAGELSDDCR
                     VHTPMGTELHQLVDGTMKDAVLNAANAKATVTFNTESIPTCSDIVKIYSDYTMKIQLV
                     GDINYTLSTSDF"
     gene            3633..4955
                     /locus_tag="PLM90_12880"
     CDS             3633..4955
                     /locus_tag="PLM90_12880"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HMZ90324.1"
                     /translation="MQLTKILICFALAIVCTTAQANPDSTATKSSAEYTYTAPTTIFG
                     IYDPLNVSLRDNRFAFTAYGDLFLASNCFSASFLQNFFGSGFITEEMKDFASEKLSAE
                     NTLGVDLSAGLWMQMAMKNNADNIFIAGIDYNFEESSQFTPDLFHLAFYGNYDLQGET
                     AILSGSKFSLTNVIEYKVGMMKLFNNGYNTYKLGITGGLVQGLSGLDIKAPTGTMYTA
                     PDGRYIDFDYDFSIKTSGKNAPDLASFVGAGFSVDLFGQAYFKGPEITINAMVNDIGA
                     VFWNNDPLKISADSTLHFEGIEINNLFGAVDTTIAGSSDSLLEILGVVEEENVFSQAL
                     PSRINVSASKFITADIYFTIGAQYILNTPYKPLIFVQAAKAIPSLKMSIAANAHAGGY
                     GVFNAGLDISKDLGSLATIRVGSNSLLGIVAPDTFRGLGAYGTLTVRL"
     gene            5073..>5521
                     /locus_tag="PLM90_12885"
     CDS             5073..>5521
                     /locus_tag="PLM90_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HMZ90325.1"
                     /translation="MNSINTDVCILGAGPGGAAAALFLAKEGIPCVIVDKAIFPRDKV
                     CGDALSGKVVEILNRYDRKLVERLSNDSIQLNCWGVTFVAPNLEELSIPFRNKPKDPE
                     KAREIAPGFISKRVDFDNFMVEELKKHGSIQLIQGVDIDKFERKEGG"
BASE COUNT         1616 a         1112 c         1213 g         1580 t
ORIGIN      
        1 ccatgcgtca ttatcgcgga aataagattt ccatgatatt tcaggagccg atgacgagtt
       61 taaatcctgt ttttacttgt ggtgcacagg tggatgaagc gataaaattg catcagaaaa
      121 aaaataaaca tgaagcacgc gcacttacca tttcattatt cgaaaaagtg caattgccaa
      181 atcctgaaag aatatatcgc gcttacccac atgaattaag tggtggtcaa aaacaacgtg
      241 tcatgattgc catggcgatg agttgcaatc cggatatatt aattgccgat gaaccaacaa
      301 ccgcactcga tgtaactgtt caaaaaacga tattgcagtt aatggaagaa ttaaaatcta
      361 ccattcattc gtctatcgtt ttcatcaccc acgatcttgg tgtgattgca gaaattgccg
      421 acagggtatt ggtgatgtat aaaggtcgca ttgttgaaga aggtacggtg ctcgagatct
      481 ttgaaagccc caaacatccc tacaccaaag ggcttatggc ctgtcgaccg ccattgggaa
      541 agcgtcttcg caagctgccg gtgattgcag atttcatgga agaaagtgcc gatggtgaac
      601 tcatagaaaa acaacaatca gtagaacagg ccgtaaaaat taatgaagta aacagcactg
      661 aaacaaaaga gcgattatca aaattaatag tacaggaacc ggttctaaaa gtgcgcaacc
      721 tgaaaacctg gttcccatcg aaaaaaagtt tcctgggaaa agttgaagga tggataaaag
      781 cagttgatga tgtatcattc gatgtttatc cgggagaaac acttggactt gttggtgaaa
      841 gcggttgcgg aaaaactacc ctcggaagaa caattatcag attggtagat ccgatttcag
      901 gtgaggtgca tttcgataat cagaatttaa tgacactcac gcatcgcgag ttaaaaccca
      961 tcagaaaaga tattcaaatt atttttcagg atccatattc cagtttaaat ccgcgcatga
     1021 cagttggcaa tgcagttcgt gagccaatgc aagtgcacgg catgtattcg agcgatcgtg
     1081 agcgaaaaga aaaagtgata gaacttctgg aaaaaacagg tttgaaagca gatcattata
     1141 atcgctatcc gcacgaattt tccggaggtc agcgccagcg tatttgcatt gccagagcct
     1201 tggcattaaa tccgaaattc attatttgcg atgaaagtgt ttcagccctc gatgtgagcg
     1261 tgcaggcgca agtactcaac ctgctcattc aattgcgcaa tgaatttaac ttctcctata
     1321 tcttcatctc ccacgacctc agtgtagtaa aattcatgag cgaccgcatg gtggtaatga
     1381 ataagggcgt tatcgaagaa atgggaccgg cagacgaaat ttacgataac ccgcaaaagg
     1441 cttacaccca aaagctgatc gacgccattc cgaagggtca gcttagtgac ataaaggggc
     1501 gcctaatgca actgcaataa agatttacgt atcttcacaa catcatgacc cgcagaccca
     1561 ttggcttcct cctcgtgata atggcactgt ttacaattgt gtcgtgccga aaagacctca
     1621 ataccggctg ggatacacgt tataaactac ctattgttac cggcgaactg gatctctacg
     1681 atcttgttcc cgactcactt accgtaatcg gcgatgatca gagcgttacc ctggtttaca
     1741 aaacagaatt gctcgattac gacctgacgg aagaagctat tgaaattcct gataccagtg
     1801 ttgaatattt tgtttcactc gattcattgt cgcttgaaga tcaagacctg actatcccta
     1861 tctcccttgg tcaaattgca atagaccttg gctttcctta tggaacgctt atcattgccg
     1921 ccaatggaac agaagttgcc ctcgatccaa tttacggtct gtcaacagac cctacccctg
     1981 ttgatgccac cacgtttttt gaaactgcga ctttccaaca tgcttatctg gatatttctt
     2041 tggcgaatgg gttacccgtc gatttagata ctgttgtttt ttctttgaaa aattcagttg
     2101 gtggtgaaac agtagtttac gatacctttt ttaccgtgcc tgccggaggc gtaccagttg
     2161 ttgaaacata cgatctttcc ggtatgaccg ttgaaggtaa cctcctcgga caaattgctt
     2221 atttcagcac accgggaagt gccgatcctg tgttaattga taccagcgat gttttggtga
     2281 tagatatgcg tgcgtatgac atggaattgc aagaggcagt tgctgttttc cctgagcaaa
     2341 atctgattaa caatgccaac gatgtgcaat acgatatggg tggccccgaa ttcactacca
     2401 tgggtattga agaaggatat gttgtcattt atgtagaaaa taccattggt gataccattc
     2461 acatcgttta tgatattccg ggggccgtag atgcattggg aaatcacgtg catattgaaa
     2521 cggctgtcgg ccctgatgaa atcgtagatc aggctttcga tattgcaggt tataccattg
     2581 atctgacagg taccagtgga aatgaagtaa atacatttta tcaggaattt agtgcacgga
     2641 taaattacac aggtattcca acacatattt ccttagacga tagtttgcgt gtgatttatg
     2701 gacttgaaaa tattgttccg aatgaattgc gcggttacct tggacaatat gaaattgcca
     2761 tggcggatac tactgcaggt gttgatcttt tcgatcaatt taccggtggc acaattgaat
     2821 tcggtgatat caatatcgat cttgaaattg aaaacggact tggagcaggt ggtgttgtta
     2881 ccattaattc tctcggtggt attaattcag aaaccggtga agtaattatg ctcgattgtc
     2941 ctgctgtgat cggcacacca atttccgtaa atcgcgcaat agataatcct tatgttccgg
     3001 gttatactac catcacactt aattcagcca attccaacat caacgacctt atcgagatgt
     3061 tccccgataa attattttat gatgtttcca tgagtgtaaa tcccaatggc aacgaattca
     3121 attatcagga ttttgtgatc agtacaagta agataaatat ctccttcaac ttaagcatgc
     3181 cgcttgagtt tatggcgaat gatcttacac tggaaagtga attccctgtc accctcgatg
     3241 cagatgcagg cacagatggt attggcgata tcgatcttac tttgtatgcc gataatactt
     3301 tcccattaaa tgcatcagtg agcatcgtat tttatgatga tttcggtgtt gcactcgata
     3361 ccttgaattt tggcggatat aatattaaag ccggtgaatt gagcgacgat tgccgtgtgc
     3421 atacaccaat gggcaccgaa ttacatcaac ttgtagatgg caccatgaag gatgccgttt
     3481 taaatgctgc caatgcaaag gcaaccgtaa catttaatac cgagtccatt cctacatgca
     3541 gcgatatcgt taagatatac agcgactata ccatgaagat ccaattggtg ggcgacatta
     3601 attacacact ctcaacttca gatttctaaa acgtgcaact gacgaagata ttaatttgct
     3661 ttgccctggc gatagtttgt acaactgcgc aggcaaaccc cgattctacc gctaccaaat
     3721 catctgctga gtatacttat acagcgccaa ccaccatttt tggtatttac gatcctctga
     3781 atgtttcact gagagataat cgtttcgctt ttactgctta tggcgattta ttcctggcaa
     3841 gtaattgttt cagtgccagc ttcttacaga atttctttgg aagtggtttt atcactgaag
     3901 agatgaaaga ttttgcatct gaaaaattat ctgccgaaaa caccttgggt gtagacctta
     3961 gtgcagggtt atggatgcaa atggcaatga aaaataatgc cgataatatt tttattgccg
     4021 gcatcgatta taatttcgaa gaaagcagtc aattcacccc cgatcttttt catcttgcat
     4081 tttatggcaa ttatgacctg cagggagaaa cagcaatatt gagcggatcg aaattcagcc
     4141 ttaccaatgt aattgaatat aaggtgggga tgatgaaatt attcaacaac ggatataata
     4201 cttacaaact cgggataacc ggcggcctgg tgcagggatt gtcggggttg gacatcaaag
     4261 ctccgacagg aacaatgtat acagctcccg acggaagata cattgatttc gattatgatt
     4321 tcagtattaa aacttcaggg aaaaatgcac ctgatcttgc ttcgtttgtt ggtgccggat
     4381 ttagtgttga tttattcgga caggcatatt tcaaaggacc tgaaataacc atcaacgcaa
     4441 tggtaaatga tattggtgcg gtattttgga ataatgatcc gttaaaaatt tctgccgaca
     4501 gcaccttgca ttttgaaggt atagagatca acaatttatt cggagcagtg gataccacta
     4561 tcgcaggatc ttcagattca ttattggaga tattgggtgt ggtggaagaa gaaaatgtat
     4621 ttagtcaggc attgccatcg cgcataaatg tcagcgcatc aaaatttatc accgccgata
     4681 tttattttac tattggtgca caatacatct tgaatacacc ttataaacca ctcatttttg
     4741 tccaggctgc aaaagcaatc ccatcattaa aaatgtcgat agctgcaaat gcgcatgccg
     4801 gtggttacgg cgtattcaat gcgggactag atatctccaa agatttaggt tctttagcaa
     4861 ctattcgtgt cggctccaac agtctccttg gcatagtagc cccggacacc ttccgtggat
     4921 taggggctta cggaacgctg actgtgaggt tgtaattaat ggtgtgcgag ggttgcacgc
     4981 agaggcgcag agccgcagag cgagcccatt actctcctat ctcctatctc ctttttcctt
     5041 tctcctttct ccttttttcc catctttgcg ccatgaacag tataaatact gatgtctgca
     5101 tattaggtgc cggtccggga ggtgcggcag cggcattatt tttggcgaag gaaggcatcc
     5161 cttgtgtgat tgttgacaaa gctatttttc cacgcgataa agtttgtggc gatgcattga
     5221 gcggaaaagt tgtggagatt ttaaatcgtt acgacaggaa attagttgag cgcttaagca
     5281 atgacagtat acaattaaat tgttggggag tgacgtttgt agcaccgaat ctggaggagc
     5341 tcagcattcc gtttcgcaat aagcctaaag accctgaaaa ggcaagagag atagcacccg
     5401 gttttatcag caaacgcgtt gatttcgaca atttcatggt ggaggaattg aaaaaacatg
     5461 gatccattca attgatacag ggtgttgata tagataagtt tgaaagaaag gagggtggat
     5521 t
//
LOCUS       DAOQOU010000194         5403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9192_length_5403_cov_4.703628, whole genome shotgun sequence.
ACCESSION   DAOQOU010000194 DAOQOU010000000
VERSION     DAOQOU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9192_length_5403_cov_4.703628"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2862
                     /locus_tag="PLM90_12890"
     CDS             <1..2862
                     /locus_tag="PLM90_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ90326.1"
                     /translation="EVAVKTFITDISHRKRTERQIIAVKEQYLNLVENITDIIFQTDN
                     DANFTFLNRVWESYTGFQIDDTIGKSCFEFLNHPQNTSLFYTKVRRLLEYGIKEFQYD
                     LLLSVKNGEPRFVQVSLKPLVDQKGLITGINGIMRDIHARKLADIEVRKIQKTLKHHQ
                     HTLASLTKEESMINGDFHGALKSISRVAAHTLDVSAVNIWKFSPDYSTLTCVINYDRQ
                     SDAYSSLQEFHINQFPAYFKLLINERFIISDDVQNDDRVIEFRDIYLIPQHVISMMDV
                     AISNGDQLWGIMCFENRHSWHKWTLEDQSFARSLTDFVSLAFKSSQLNTAESALREKE
                     YHYTNLVEQAQDAIMIIDADDNFLEVNDAACRISGYAKEELLAMQVGQILPKGRIEFY
                     RKPNNDTVENKYLFGEQTIIRKDGTERTVEISARVFSDGRLQGIVRDVTERKLQDKAL
                     RESEARLDLALKGAEMGIWDFYIRENKMVHNKRWAEMLGYYFEDTVVTEEYWDKFIHP
                     DDRDKAYANFDEHIKGKRPIYESEIRMLASDGQWRWILDKGRVVEWDAEGKPLRASGI
                     HQDITAIKTYQQQILNQAGFMQQLVNAIPNLIYVKNAKNEFITVNNALAGFLGTTAED
                     LLQYPHGQQRNYSAVLNQLTACDSEVFLKKDIIITHEEEIPDVQTGRLIWLKSIKVPL
                     IDHQGVLSEVLSVSMDVTALKQKENEISLLNDGLEIKVLERTNELEIANKELETFNYS
                     VSHDLRTPLRSIDIFAYLLDKYNKETLDPEALENIRQIRSSVVKMSTLIDNLLIFSKM
                     GRSDKRMDIVPVQKLVEEVLSELNKHADLSHYTILVRDLPVLTGDYDMLRQALMNLVS
                     NAIKYTQTRKRPIIEFFSTSDEQAHTVAVRDNGVGFSAELKDKLFQAFRRLHSEEQFD
                     GTGVGLAIVERIIKRHNGRVWAESEEGQGSTFYFSIPRG"
     gene            2950..3031
                     /locus_tag="PLM90_12895"
     tRNA            2950..3031
                     /locus_tag="PLM90_12895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2984..2986,aa:Leu,seq:cag)
     gene            complement(3080..5365)
                     /locus_tag="PLM90_12900"
     CDS             complement(3080..5365)
                     /locus_tag="PLM90_12900"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ90327.1"
                     /translation="MKRLFTSLLTLMSVYALSGQSGTIRGFVYDIENGEPVPFVNVIV
                     MQSDNIGAASDENGFFSIPEVPVGAQRLILTYIGYDTLIHPITVEAGQIYNVKLNMTQ
                     SGISLSTVTVSSKVTKKLEEVQISTTTITPLQIKRLPSVGGEPDLAQYLQVLPGVIFT
                     GDQGGQLYIRGGSPIQNKVLLDGMVIYNPFHSIGLFSVFETDIIRNVDVYTGGFSAEY
                     GDRVSAVVDITTRDGNKKQTVGEVSASPFLARVLLEGPLKKPSAESSSVTYILTAKHS
                     YLDKTSEIFYPYVNSQEGYSLTQGIPYTFTDLYGKISINSESGNKFNVFGFNYRDSVN
                     YSSITNYRWTASGAGTNFVIVPSASNILIEGVFAYSNYNMTQNEADEKPRTSNVGGFN
                     GGVDFTYYLGSGDLQYGVEVIGFNTNYEFFNRLGIRTGQEQFTTELGSYVKFRKSFNK
                     KFVIEPSIRLQYYASLSEFSPEPRIGMKYNITDRVRIKFAGGLYSQNLISSKSDRDVV
                     NLFTGFLSGPDDDLYTIDGEEAKSKLQRSTHAIAGIEYDPTDFIELNIEPYIKYFNQL
                     IEINRNKVTPRDADFATETGQAYGIDFSMKYDRKKLYIWLAYSLGFIERNDGEQVYPP
                     HYDRRHNMNFVTTYTLGKRWELSARWNLGSGFPFTQTAGFYESVDFLDGISTDYTGTN
                     GNLGIIYSAQLNGGRLPYYHRLDLAVKHNIVFANKNKVEINAGVTNVYNRDNIFYFDR
                     VRYERVNQLPVLPSLSMSYSF"
BASE COUNT         1656 a         1071 c         1130 g         1546 t
ORIGIN      
        1 gaggttgctg tgaaaacgtt tatcacggat atcagtcacc gtaaacgcac cgaacgacaa
       61 atcattgctg ttaaggaaca atacctgaat cttgtagaaa atataaccga catcattttt
      121 caaacagaca atgatgccaa ttttacattt ttaaatcgtg tttgggagtc ttacacagga
      181 tttcagattg acgataccat cggtaaatcc tgtttcgaat tcttgaatca tccgcaaaat
      241 acatcccttt tttataccaa agttcgtcgc ttattggaat atggtatcaa agaatttcaa
      301 tatgatttat tgctgtcggt aaaaaacgga gaaccgcgat ttgtgcaggt aagtttgaaa
      361 ccactggtag atcagaaagg actgatcaca ggcatcaatg gtatcatgcg cgatattcat
      421 gccagaaaac ttgctgatat tgaggtgcgg aaaattcaga agactttaaa gcatcatcaa
      481 catacattag catcactcac caaggaagaa tccatgatca atggcgattt ccacggtgct
      541 ttaaaatcca tttcccgcgt tgctgctcat acgcttgatg tgagtgcggt gaatatttgg
      601 aagttctctc cagattattc aacgcttacc tgcgtaatta attatgatcg ccaatctgat
      661 gcatacagtt ctttacaaga atttcatatc aatcagtttc ctgcatattt taagttgtta
      721 ataaatgaac gatttatcat ttcagatgat gtgcagaacg acgatcgcgt aatcgaattc
      781 agagatatct atttgatacc acaacatgta atttccatga tggatgtagc tattagcaat
      841 ggagaccaat tgtggggtat catgtgtttt gagaacagac acagctggca taaatggact
      901 ttagaagatc aatcttttgc acgcagtctt acagattttg tttcgttggc atttaaaagc
      961 agccagttga acactgctga atctgctctg cgtgaaaagg aatatcatta taccaatctt
     1021 gtagaacaag cccaggatgc tataatgatc attgatgctg atgataattt tctggaagtg
     1081 aacgacgcgg catgccgaat ttccggctat gcaaaagagg aattacttgc catgcaggtg
     1141 gggcagatat tgccaaaggg gcgaattgaa ttttatcgca agccaaacaa cgacactgtt
     1201 gaaaataaat atttgtttgg cgaacaaact attatacgca aagatggaac ggagcgtacc
     1261 gtggaaatca gcgcacgcgt attctccgat ggcagattgc aaggcatcgt acgcgatgta
     1321 acagaaagaa aattgcagga taaagcactt cgtgaaagtg aagctcgcct cgaccttgca
     1381 ctgaaaggtg ccgaaatggg tatttgggat ttttacattc gtgaaaacaa aatggtccac
     1441 aacaagcgtt gggctgaaat gttgggatac tattttgaag atacggtagt gacagaagaa
     1501 tattgggata aatttattca tcctgatgac cgcgataaag cctacgccaa tttcgatgaa
     1561 catattaaag gaaagcggcc gatttatgaa tcagaaattc gcatgttggc tagcgatggc
     1621 caatggcgat ggatattgga taagggaagg gtagtggagt gggatgcaga aggtaaacct
     1681 cttcgtgcat cgggaattca tcaggatatt actgccatca aaacctatca gcaacaaata
     1741 ttgaatcagg ccggattcat gcagcaactt gtcaatgcca ttccaaacct gatttatgta
     1801 aaaaatgcaa aaaacgaatt cattaccgta aataacgcgc ttgcaggatt tctgggaact
     1861 accgctgaag acttgttaca atatcctcat ggtcaacaac gcaattacag cgcagtacta
     1921 aatcaattaa cggcctgcga tagtgaggtg ttccttaaaa aagatatcat cattacgcat
     1981 gaggaagaaa tacctgatgt gcaaacaggt agattgattt ggctgaaatc tattaaggtg
     2041 ccattgatcg accaccaagg tgttttatct gaagtgcttt ctgtatcaat ggatgttacc
     2101 gcacttaagc aaaaagaaaa tgagatcagt ttgcttaatg atggactgga aattaaagtg
     2161 ctggagagaa cgaatgaatt agagattgcc aataaagagc tggaaacatt caactattct
     2221 gtatcgcatg atttaagaac accattgcgt tcgatagata tttttgctta tctgctagat
     2281 aaatataaca aagaaacgct cgaccccgaa gcgcttgaaa atatccgcca gattagaagc
     2341 agcgttgtaa aaatgagtac cttgattgac aacctgctca ttttctccaa aatgggtcgc
     2401 agcgataaac gaatggacat cgttcctgtg cagaaattgg ttgaagaggt gttgtcggaa
     2461 ttaaataagc atgccgacct ctcacattat accattttag tacgcgacct tccggttctg
     2521 acaggtgatt atgatatgct gcgtcaggca ttgatgaatt tggtaagcaa tgccataaaa
     2581 tatacccaaa cacggaagcg acctatcatt gaatttttct ctacttccga tgaacaggca
     2641 catactgttg cagtaagaga taatggtgtt ggtttcagtg cggaactaaa agataagctt
     2701 ttccaggcat tccgccgcct gcatagtgag gagcaatttg atggcaccgg cgttggtctg
     2761 gcaatcgttg agcgcatcat taaacgtcac aacggtcgcg tttgggccga aagcgaagaa
     2821 gggcagggta gcacattcta ctttagcatt ccgcgtggat aataccgctt gtaaacggcc
     2881 ggcatcccat ctgcatgctg aacatatttt gcgattttta ataaaatagc ctagctttgc
     2941 actccacaaa ccggagtggt ggaattggta gacacgcacg tttcaggggc gtgtgccgaa
     3001 aggtgtgcgg gttcgagtcc cgcctccggt acataaaaaa agacagcata ttggctgtct
     3061 ttttttatat agtttatcat tagaacgaat aactcataga tagcgacggt aaaaccggta
     3121 gctgatttac gcgttcataa cgcacacggt cgaagtagaa aatattatcg cgattgtaca
     3181 cattcgtaac ccctgcattg atctccactt tatttttatt cgcaaataca atattatgtt
     3241 ttactgccag gtccagacgg tggtaatacg gcaagcgacc gccattcaat tgcgctgaat
     3301 aaataattcc aagatttcca ttggtgccgg tgtaatcagt gctgatgcca tccaggaaat
     3361 caacagattc ataaaatcct gcagtttgtg tgaatgggaa tccagatcct aaattccaac
     3421 gtgcacttaa ttcccagcgt ttacctaatg tataggtggt aacaaagttc atgttatgac
     3481 ggcgatcgta atgcggcgga tatacttgct cgccatcatt gcgttcgata aatccgagtg
     3541 aatacgccag ccagatatat aattttttgc gatcatattt catgctgaag tcaatgccgt
     3601 atgcctgacc ggtttccgta gcaaaatcag catcgcgtgg tgtaaccttg tttcggttga
     3661 tttctatcag ctgattgaaa tatttgatat aaggttcaat attcaactca ataaaatcgg
     3721 taggatcata ttcaataccg gcaatagcat gtgttgaacg ctgtaatttc gatttagctt
     3781 cttcgccatc gatggtatac agatcatcat caggacctga taagaaacct gtaaataaat
     3841 tcaccacatc gcgatcgctt ttgctgctga tgaggttctg actgtaaagt ccgcccgcaa
     3901 actttatacg aacgcgatcc gtaatattgt atttcatacc aatgcgcggc tccggtgaaa
     3961 attcactaag tgatgcatag tattgtaaac gaatactcgg ttcaatcaca aacttcttat
     4021 taaatgattt tctgaatttc acataagagc ctaactcagt cgtaaattgt tcttgccctg
     4081 tgcggatacc aagtctattg aagaattcat agttggtatt gaaaccaatt acttcaacac
     4141 catattgtaa atcgcctgaa ccgaggtaat atgtaaaatc aacaccgccg ttaaatccgc
     4201 cgacattact ggtgcgcggt ttttcatcgg cttcattctg tgtcatattg taattgctgt
     4261 atgcaaaaac accttcaatc aaaatattgg atgctgaagg aacaatcacg aaattagttc
     4321 cggcaccact ggctgtccag cggtaatttg tgatggatga ataatttaca ctatcgcgat
     4381 aattgaatcc aaaaacattg aatttatttc cggattctga attgatggaa attttaccat
     4441 ataaatctgt gaatgtatat ggaatacctt gtgtgagcga ataaccttct tgactattta
     4501 catagggata aaatatttcc gaggttttat cgaggtagct gtgttttgct gtgaggatat
     4561 aggttacgct gctggattcg gcacttggtt tttttaatgg accttctaat aacacacgag
     4621 ccaaaaacgg actggcagat acttcgccaa cggtttgctt tttattaccg tcacgcgttg
     4681 taatatctac aacagctgaa accctgtcgc catattcagc actaaatcct cccgtataca
     4741 cgtcaacgtt tcttatgata tctgtttcga aaacactgaa taaaccaatg gagtgaaaag
     4801 gattataaat caccatacca tccaataaca ctttgttttg aatcggacta cctccgcgta
     4861 tatacaattg ccctccctgg tcgcctgtaa aaatcacacc gggtaaaact tgcaaatact
     4921 gcgcaagatc cggttcgcca ccaactgatg gtaaacgttt gatttgcaaa ggtgtgatcg
     4981 tggtagtaga aatctgcact tcttccagtt tcttggtaac cttgctcgaa acagtaactg
     5041 tactcaaaga gatccccgat tgggtcatat tgagcttcac attataaatc tgacctgctt
     5101 caactgtaat tggatgaatg agtgtatcat atccgatata ggtgaggata agtcgctgcg
     5161 caccaacagg tacttcaggt attgagaaga acccgttctc atcggaggca gccccaatat
     5221 tatccgattg catcacaatc acattcacaa agggaaccgg ttcaccattt tcaatgtcgt
     5281 aaacaaatcc gcggatggtg cccgattgtc ctgaaagggc gtaaacactc atcagcgtaa
     5341 ggagagaagt aaataatctt ttcatatttg tttaatgtac taaattttat attttcatta
     5401 cat
//
LOCUS       DAOQOU010000195         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9215_length_5397_cov_4.797267, whole genome shotgun sequence.
ACCESSION   DAOQOU010000195 DAOQOU010000000
VERSION     DAOQOU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9215_length_5397_cov_4.797267"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLM90_12905"
     CDS             complement(<1..368)
                     /locus_tag="PLM90_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ90328.1"
                     /translation="MEVIEFLDDSGTVLVKRMPDNGQLEIKWGSQLTVRESQDAVFFR
                     DGKSLDVFTAGRHVLKTQNVPVVGKWVTSFGYGENSPFRAEVLFVGKQLFPNFKWGTK
                     EPILFKDTELQMVRLRSYGS"
     gene            complement(372..854)
                     /locus_tag="PLM90_12910"
     CDS             complement(372..854)
                     /locus_tag="PLM90_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90329.1"
                     /translation="MTKRNVNFFDKIGTLIPGYNGYAARDEKRNDDKKLRDELSRIIN
                     QAETLIEKHQQQLIKTGEINLCKEWEIARKSVNTLYIKVKNATYGESSFFSNNQIKET
                     ELDEIYNFDYEMAERVHLIFKTADADLNEVMSAGLIINQVKEIDNILNKRTTFINRHK
                     "
     gene            1267..2112
                     /locus_tag="PLM90_12915"
     CDS             1267..2112
                     /locus_tag="PLM90_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMZ90330.1"
                     /translation="MADIQQLEKIASQVRRDIVRQVYNCQSGHPGGSLGCTDFLVALY
                     FDVMQHDPKFNMNGSGEDMFFLSNGHISPVWYSVLARSGYFPVAELNTFRKLNTRLQG
                     HPTTAEHLPGIRMASGSLGQGMSVGIGAALSKKLNNDPHLVFTLHGDGELQEGQIWEA
                     AMFASHKQVDNIICTVDWNGQQIDGPTKKVMDLGDLHAKFSAFGWDIVMLEKGNDMAS
                     VVAALHEAKSRTGKGKPVMILMKTAMGAGVDFMMGSHEWHGIAPNKEQLEKAMAQLTE
                     TLGDF"
     gene            2194..4206
                     /locus_tag="PLM90_12920"
     CDS             2194..4206
                     /locus_tag="PLM90_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90331.1"
                     /translation="MRQKKKDRSAQLLEYSALSLAFIGLPAIADAQVVYVDVDPDTTT
                     YLDEYELDMDNDGSVDFKLIFDTDIFATFAPPELKLRINPLGSNSVAFLYQSIPVTYS
                     GMPFSTYIDGATAIFFTDVALLNDGDVIDNALNFQLEKAALYSRRFFFIYSESNYAAF
                     GGGYWNETTDHFAGFRLFTDGAYHYGWIRLNVSADSGITLLDYAYELNPETPLTAQVR
                     DYKISWVSVGDAGITETTADLAFSFNAAVDQADIASYRIFCVPTADVNDMTPDIAASL
                     SPDRYVEIIPDGSDVYSGNFSGILLDSNGDPIVEGVNYKLMAVNIMLNNYLPILSVPT
                     PKFSLVNTVQMPLNVTLSDLNNTGTGEDVRVKFDAPSITQGISSYRIIILPRDSADAF
                     TLDEALLVTPENFTEVAVGDADYAVMLNSTSTDAYGNLIYLEKYKAVVLSMADDVTVA
                     HDAMSDVSGVVVLETATAVASGVTLSDVNETASGFDLHVSFNYGTPEQTVATYQLYFV
                     DYATAFDFDVAAALASPNFISFTPDNVDFTFTGNDTLRDSDGDLISWGVPYYAWVLSV
                     SSDFGVSDTLSEPSNQVILNFPVGIETSDAATDQLSYSNGQFRVSCSEEQTLSFYNLS
                     GELISSQVIFPGITNLPVDLPAAMYLARLSNGKSLSVLPFVVTGTH"
     gene            4363..>5397
                     /locus_tag="PLM90_12925"
     CDS             4363..>5397
                     /locus_tag="PLM90_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ90332.1"
                     /translation="MKKLFLLVCIPLLIGMTAAVGRAQTSPPAATKRILILLDGSGSM
                     VDPWNGTNKWEVAKKLVTKTIDSIQRSEPGVEIGLRVFGFQQPRAVHDCKDSKLVVPI
                     GKNTGSAVKSALASITPQGYTPIAYSLFLAAGDFPTAEAANSIILITDGIENCDGDPC
                     ASTQVLRDKRITLKPFIIGLGIEEKDKKQFECVGAYYDASNEKTFANAMSIVISQALN
                     ITTTQINLLDAFGLPLEKNIEITLYDHASGELRYNYVHTPDDKQQPDTLFLNPVGKYD
                     ITVHTTPPIFLNDVELTPGKHNIIGIDVPLGTLVLSEGGNTTLSEKQLVLRDNETGVI
                     IYVQNFNTKMK"
BASE COUNT         1430 a         1137 c         1147 g         1683 t
ORIGIN      
        1 aatgaaccat aggaacgcaa tcgcaccatt tgaagttcgg tatctttaaa taaaatgggt
       61 tcttttgtac cccacttgaa atttgggaat aactgcttac cgacaaacag cacttctgct
      121 ctgaatggtg aattttcacc atatccaaat gacgttaccc atttacctac tactggtaca
      181 ttttgtgttt ttaatacatg tcttccagct gtaaaaacat caagagactt tccatctctg
      241 aagaaaacag catcttggct ctctctaacc gttaattgag agccccattt aatttcaagt
      301 tggccgttgt cgggcattcg ttttactagt acggtgccac tatcatctaa aaattcgatt
      361 acttccattt tttatttatg tctgtttata aaagtggtac gtttgttaag aatgttgtct
      421 atttctttta cctggttaat tattagacct gcactcatta cttcattaag atcggcatct
      481 gcggttttaa agattaaatg aactctttcg gccatttcat agtcaaaatt ataaatctca
      541 tccaactcgg tttcttttat ttggttgttg ctaaagaaag aagattcgcc ataagtagca
      601 ttttttactt ttatgtaaag agtatttacg gactttcttg cgatttccca ttccttacac
      661 aaattaattt cgccagtctt gattagttgt tgttgatgtt tttcaatcaa tgtttcggct
      721 tgattaataa ttctggacag ttcatcacgc aattttttgt cgtcatttct cttttcatcc
      781 cgagctgcat aaccattgta gcctggtatt aaagttccga ttttatcaaa aaaatttaca
      841 tttctttttg tcatattttt atatgttact catttttatt cgtttgcaaa tgtacatctt
      901 tagtctaaca gggttgcacc taacttactt atagccgcaa cccaatttcg ccaagcaccc
      961 caaatcatct gttaaagcga aacaaatgtt aatttttaag cgacctattg cttacaaatt
     1021 tgcacttcca tcagcattcc ccattcacgg aaaaccgtta atccccgcat gtcacaaatt
     1081 ctgtaaaatt taaagtgaaa atgcccatat ttcaacactt ccggctatta gcagcttgtt
     1141 ttcctggtat ttgtgatgtc acatttcgcg attggccttg cgggtagttt tgccatcgcc
     1201 ctacccacac ctgtgcaaag cgccttttgg ggaatgcctt atctttgccg aaacttaagc
     1261 ttttacatgg ccgatataca gcaattggaa aaaatcgcat ctcaggtgcg cagagatatt
     1321 gttcgtcagg tgtacaactg ccaaagcggt cacccggggg gctcattggg atgcaccgac
     1381 tttttggtgg cgctgtattt cgatgtgatg cagcacgacc ccaagtttaa tatgaacggc
     1441 tccggtgaag acatgttctt ccttagcaat ggtcatatct cgccggtatg gtatagcgtg
     1501 ctcgccagaa gcggttactt cccggtggct gaactcaata ctttccgcaa gctgaatacg
     1561 cgtttgcaag gacatcctac caccgccgag cacttgccgg gtattcgcat ggcttcaggc
     1621 tcgctgggtc agggaatgag tgtgggtatt ggtgctgcgc tctccaagaa gttgaataac
     1681 gacccgcatc tggtgtttac cctccatggc gatggcgaat tgcaggaagg tcagatatgg
     1741 gaagccgcca tgtttgcctc tcacaagcaa gttgataata tcatctgcac tgttgattgg
     1801 aacggtcagc agattgatgg tcctaccaaa aaggtgatgg atctcggcga tctgcatgca
     1861 aaattctctg ctttcggctg ggatatcgta atgcttgaaa aaggcaatga tatggcatct
     1921 gttgttgctg ccttgcatga agcgaaatcg cgcaccggaa aaggtaagcc tgtgatgatc
     1981 ctgatgaaaa ctgccatggg tgcaggggtt gatttcatga tgggcagtca cgaatggcat
     2041 ggcatcgctc ctaacaagga acagcttgaa aaagccatgg cacagctcac agaaacgctc
     2101 ggcgatttct agtatatctt tgaatgatta ataaagtcgc ggatttgtgc ggcttgcaat
     2161 tacatcatca tgtcgttttt gtaattttaa accatgcgac aaaagaaaaa agatcgttcc
     2221 gctcaattgc tggagtattc tgctctgtcg ctcgcattta tcggactccc tgcaattgct
     2281 gatgctcagg tagtatatgt tgatgttgat cctgacacta ccacttatct cgacgaatat
     2341 gaactcgata tggataatga tggcagtgta gattttaagc tgatttttga cactgatatt
     2401 ttcgccacat tcgcgccacc ggaattaaaa ctccgcatta accctctcgg cagtaatagt
     2461 gttgcatttc tttaccaatc aattcctgta acgtattcgg gaatgccatt ttctacctat
     2521 atcgatggtg caacggcaat ctttttcaca gatgtggcat tattgaatga cggtgatgtt
     2581 atagataatg cgttgaattt tcaattggaa aaagcagcat tatattcgcg acgatttttt
     2641 ttcatctatt ctgaatctaa ttacgctgca tttggcggtg gttactggaa tgaaactacc
     2701 gatcattttg ccggcttcag attattcacc gatggcgctt atcactatgg gtggatacgt
     2761 ttaaatgtca gcgctgacag tggcattaca ttgttggatt atgcctatga attaaatccc
     2821 gaaacaccgc tcacagccca ggttcgcgat tataaaataa gttgggtatc tgtaggagat
     2881 gcaggaatta cagaaacaac tgccgacctt gcattctctt tcaacgcagc cgttgaccag
     2941 gctgatatcg catcgtatcg tattttttgt gtgcctacgg cggatgtcaa tgatatgacg
     3001 cctgacatcg ctgcttcgtt atcgcctgat cgttatgttg aaattattcc cgatggcagt
     3061 gatgtgtatt ccggtaattt cagcggcata cttctcgaca gcaatggcga ccctattgtt
     3121 gaaggtgtga attataaatt gatggcagta aatattatgt taaacaatta cttgccgatt
     3181 ttatctgttc ctactccaaa attctcattg gtgaatacgg tgcagatgcc attaaatgtt
     3241 acattatctg atttaaacaa taccggcacc ggtgaagatg tgcgcgttaa attcgatgcg
     3301 ccttcaatca cgcagggtat cagtagttat cgcattatta tattgccgcg tgatagcgcc
     3361 gatgctttta cattggatga agcactattg gtaacacctg aaaattttac tgaggttgct
     3421 gtcggtgatg cagattatgc tgtaatgctt aattctacct caacagatgc atatggaaat
     3481 ttaatttacc tcgaaaaata taaagctgtt gttttatcta tggctgatga tgttaccgta
     3541 gcacatgatg ccatgagtga tgtgtcggga gttgtagtac tggaaacggc aactgctgtt
     3601 gcatccggtg ttacgttgag tgatgtcaat gaaacagcca gtggtttcga cctgcatgtt
     3661 agttttaatt atggaacgcc ggaacaaaca gtagcaacct atcagcttta ttttgttgat
     3721 tatgcaacag cctttgattt tgatgttgca gctgcgttgg catcgccaaa tttcatttct
     3781 tttacgcctg ataatgtaga ttttaccttc accggaaatg ataccctgcg cgacagcgat
     3841 ggcgatctga tttcatgggg tgttccctat tatgcctggg ttttatccgt ttcgagcgat
     3901 tttggggtga gcgatacctt atctgaacct tcaaatcagg tgatcctgaa cttcccggta
     3961 ggtattgaga cttccgatgc tgccacagat caactaagtt attccaatgg tcaattccgg
     4021 gtgagttgct cggaagaaca aaccctttcc ttctacaatt tgtccgggga gctgatttct
     4081 tctcaagtaa tttttccggg tattactaac cttcctgtcg acctgccggc tgccatgtac
     4141 ctggcccgtc tgagcaacgg aaaatcctta tctgtgctgc cttttgtggt taccggcact
     4201 cactaatagg aggagttgaa cagtatgcta tacacccttt cccgctagtg ttgatctgta
     4261 ttttaacact tttggtcaaa aacggcagca tttttgttta tttgcgtatg acccctccaa
     4321 taaggcgctg aatttctgcg ttttataaga ggcgcttttt acatgaagaa actctttctg
     4381 cttgtctgta ttcctttgct cattgggatg acagctgctg ttggtcgggc tcaaacctcg
     4441 ccaccggcag ctaccaagcg cattctcatc ctcctcgacg gctccggcag tatggtagac
     4501 ccatggaatg gcacgaacaa atgggaagtt gcgaaaaagc ttgttacaaa gactatcgac
     4561 agtatacagc ggtctgaacc cggtgttgaa ataggtttga gggtattcgg ttttcagcaa
     4621 ccacgggcgg tgcacgattg taaggactca aaattggtgg tgccgatcgg gaaaaatacc
     4681 ggtagcgcag taaaaagtgc cttggcatcg atcacaccgc aagggtatac gccaatcgct
     4741 tattcactct ttttagccgc cggagatttt cctacagcag aagctgccaa ctccatcata
     4801 cttatcacag atggtatcga aaattgtgat ggtgatccat gtgcgagcac acaggtgttg
     4861 cgtgataaac gcattacact caagccattt attatcggcc tgggaattga agaaaaagat
     4921 aaaaaacagt ttgaatgtgt cggtgcctat tacgacgcat cgaatgaaaa aacatttgcc
     4981 aatgccatga gcatagtaat ttctcaggca ttgaatatca ctaccacgca gataaattta
     5041 ttagatgcat tcggcttgcc tttagagaaa aatattgaaa tcaccttgta tgaccatgcc
     5101 agtggcgaac tgcgctataa ttatgtgcat acccctgatg ataaacaaca acctgacacc
     5161 ttgtttttaa atcctgtagg taaatatgac attaccgtac atactacacc accaatattt
     5221 ttgaatgatg tggaattgac acccggcaaa cataatatta tcgggattga cgtgccatta
     5281 ggtactttgg ttttgagcga gggtggaaat acaacattga gtgagaaaca attggtgctg
     5341 cgcgataatg aaacaggtgt gattatttac gtgcagaatt ttaataccaa aatgaaa
//
LOCUS       DAOQOU010000196         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9320_length_5354_cov_6.039253, whole genome shotgun sequence.
ACCESSION   DAOQOU010000196 DAOQOU010000000
VERSION     DAOQOU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9320_length_5354_cov_6.039253"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..4793)
                     /locus_tag="PLM90_12930"
     CDS             complement(180..4793)
                     /locus_tag="PLM90_12930"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HMZ90333.1"
                     /translation="MTTVLLLFLTIFLLLRIPAVQNWVAEKTTSWLSEKLDTEVSVKE
                     VSIDLLDHLILEGLYIEDKQDDTLLYAGKLEVNIGTINPLVPYVKIKSIGLYDTRVYL
                     DRTLPDSIYNFDFISEAFSSNDNKDTSTSSSGGGRSVQLKLNRLDLKNVHFVLHDEVA
                     ASDMQMHVGDLELLINTLDLEDHIIALEKLQIDDTRFALTGLTDTIPSNTPESWDTVH
                     IAMGDWVMEASQLKLHNCAFAFLDVNDTTPNRGINFSDMDITGIDLEMTDIIYHGDTI
                     MNRIDHLALTEKSGFRVDTLHANVLFSPYEISLTDLLIKTPNSKIQKSFAMTFTTLND
                     FDRFESDVRMDGNFDKTIVGNKDLAYFAPVLDKYDAHLLLSGHIYGALDNLKGRDFDA
                     MLNESAGLRGTLNVKGLPNIDETFIDMELEPLYVNTYRLENIIGAGAIPDNLLALGTI
                     NYTGRVTGFAYDLVTLGNLTSDQGNMYTDVHFQYDPDLGKSSFRGDLNTSAFNVGIIA
                     GEPDLLGRISMHATIDGSIARDNEMEMDMQSEISMMEFNDYTYSNMVVDGNLENDYFS
                     GNFTIDDPNVKLTFNGIINMQDSIPLYDFKANIARANLKQLNFYPEPIVFSATASADA
                     GGTSLDNIEGNLHFGDLLLIRDKYIYRLDTLNFTAAQIGDKKSIKANSTLIDLQLEGN
                     FLPSKLDESIKSLVDYYTNGRIDSTLEVQKANYTVSIKNADRLMAIFYPDIQEVRNLT
                     LSGDFNTATRSFNNRLRIDQFNYQNITLDTLLFEARTANDALEYFTRINATHIGSAVV
                     LPVLRSEGNFAQNTLNYNFKAGKDTDSNRVNLNGAVAFIDSSIMFNILPSEIFFEAEK
                     WDILPNNSLKYDNKNLYAQNFTLNSGNKVISLSSSADATYGTVLKLGIRNIPIGELAE
                     RFVLPGEDISGTLDATYTIGNALKDPSFLGGAEIKDLTLNHVLLGNLKVNTSLIQPNN
                     VLKFNSTLSGENGFVTDGYYIMGRGEDEDSLRLKADFRHNKLFIVEPFLRGILSDMSG
                     DIYGTINIDGPVSTPQMQGSLQVKNGGMTVDYMGTHYAFNQIDINVEKNKITIPETTI
                     TDKLNNTASLKGEVTYSHFDNWNFKELHVISDNLVLMETNAKQNPDFYGYAIGEVDCN
                     ITGKLDALLITVDATPHSGTVVNLPTYGSGNVKKHDFIRFVNKQIINTIETADELNLA
                     IVDVALKLNATPDAQIKLLINSEGTEFLSGRGFGALTINANSLGKVDMVGTYRITEGL
                     YDFSFQGFVPRPFTVIPGSTIEFSGSPYKAQLDLTAEYTAENVSINTLTGTDTKETAN
                     VNVLINIDGVLESPEISFDLEVADGNAKNNADFERRINEIKSDKNELNKQVFGLLIME
                     GFLPQDLTAFNAVGTTASSTMNDFFSSQLTTYFQNVLDDFIKDTEIDFGIDNIQSGNY
                     NVTTDLGNKLQLDIKREVTDNLIVKVGTTYYDFTGNSSGNANNLAGDFEVEYIISPDG
                     RVRVKAFRISEYDAIVAKNDVKTGVGIYYSKDFNKLKELFKKQ"
     gene            4905..>5354
                     /locus_tag="PLM90_12935"
     CDS             4905..>5354
                     /locus_tag="PLM90_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMZ90334.1"
                     /translation="MPVTILGIESSCDETAAAVLINGQIMSNVIAGQSVHEQYGGVVP
                     ELASRAHQANIVPVVTVALSRAGISLADLDAVAFTQGPGLLGALMVGTAYAKALAISL
                     DIPLITVHHMQAHILAHLIEDPKPAFPFLCLTVSGGHTQIVRVNSAMD"
BASE COUNT         1475 a         1075 c         1136 g         1668 t
ORIGIN      
        1 aagacgcgaa gaggcgaagg cgcgaaaatt aaagcacaac aaacacgaac acaaaaacac
       61 acaagggtat tgtcagtgat gtggctgcgc tccatgtggt aatgtgctag tgagcaaagt
      121 caggcgagtt aaacgctcaa ggtgcctttc aatttacata atcagagctg tgtgagagct
      181 tattgctttt taaacagctc ttttagtttg ttgaagtctt tagaataata aatacccaca
      241 cctgttttaa catcgttttt agcaacgatg gcatcgtatt cagaaatacg gaatgctttt
      301 acacgaacgc gaccatcggg cgaaataata tattctactt caaagtcacc ggcaaggtta
      361 ttggcattac ctgaagagtt tcctgtaaaa tcataatagg ttgttcctac ttttacaatg
      421 aggttatcag ttacctcgcg tttaatatcc aattgtaatt tattaccaag gtcggtggtt
      481 acgttataat taccactctg aatattatcg ataccaaaat ctatctctgt gtctttgatg
      541 aaatcatcca gcacattctg gaaataggta gtaagctgac tggagaagaa gtcgttcatg
      601 gtactgctgg cagtagtacc aactgcgttg aaggctgtca gatcttgtgg cagaaatccc
      661 tccatgataa gtagaccgaa cacctgttta tttaattcat tcttatcgct tttgatttca
      721 ttgattctgc gttcgaaatc ggcattattt tttgcattgc cgtcggcgac ttccagatca
      781 aatgaaattt caggtgattc cagtacgcca tcgatattga tgagcacatt gacgtttgcg
      841 gtttctttgg tatctgttcc ggtgagggtg ttaatgctca cgttttcggc ggtatattca
      901 gcagtgagat ctagttgcgc tttgtaaggg ctgccactga attctattgt gcttcccgga
      961 atcacagtaa atggtcgcgg aacaaatccc tggaagctga aatcatataa accttctgta
     1021 atacgatatg tgcctaccat atctactttt cctaaagaat tggcgttgat ggtaagtgca
     1081 ccaaaacctc ttccagacaa aaattccgtt ccttcactat tgatgagtaa tttaatttgt
     1141 gcatcaggag tggcattcaa ttttaatgca acatccacaa tggctagatt gagttcatcg
     1201 gcagtttcaa tagtatttat gatctgtttg tttacaaagc gtatgaagtc atgttttttc
     1261 acattacctg aaccataagt tggcaggttc actaccgttc cggagtgtgg tgtggcatct
     1321 acagtgatca acaaggcatc aagttttcct gtgatattgc aatctacttc accaatagca
     1381 tatccataaa aatcaggatt ttgcttggca ttggtttcca tcaataccag attgtcggaa
     1441 atgacatgta actctttgaa attccagtta tcgaaatgac tgtaggtgac ttcacccttc
     1501 agcgatgcag tattatttaa tttatcggtg atggtagttt ccggtatggt gatcttgttc
     1561 ttttctacat tgatatcaat ctgattaaat gcataatgcg tgcccatgta gtcaactgtc
     1621 atgcctccat tttttacttg gagtgaccct tgcatttgag gtgtgcttac agggccatcg
     1681 atatttattg tgccatatat atctcctgac atatcggaaa gtatgccacg caaaaatggc
     1741 tcaacaataa ataatttatt gtgacggaaa tcagctttta gtcgcaggct atcttcatct
     1801 tcaccgcggc ccatgatata atatccatcg gtaacaaatc cattttcacc actaagcgtt
     1861 gaattaaatt tcaatacatt attgggttga atcaatgatg tatttacctt gagattaccg
     1921 agcagtacat gatttaatgt aagatctttt atttcagcac caccaagaaa gcttggatct
     1981 ttcaacgcat tgccaatagt gtatgttgca tcaagtgttc cgctgatatc ttctcccggc
     2041 aatacaaagc gctcagccaa ttcgccaata ggaatattgc ggatgccaag ttttagcaca
     2101 gtgccgtaag tggcatctgc actgctgctc aggctgatta ctttattgcc gctattaagg
     2161 gtgaaattct gtgcatacag atttttatta tcgtatttca aggagttgtt gggaaggatg
     2221 tcccattttt ctgcttcaaa gaaaatttca gacggaagga tattgaacat aatgctcgaa
     2281 tctataaagg caacggcacc atttagattc acgcgatttg aatcggtatc tttcccggct
     2341 ttaaaattgt aatttaaggt attttgagcg aagttgcctt cagacctcag gacaggtaat
     2401 actacagctg acccgatatg cgtagcatta atgcgtgtga aatattccag agcgtcatta
     2461 gcagtgcgtg cttcgaacag caaggtgtca agcgtaatat tctgataatt gaattgatcg
     2521 atgcgcagcc tgttgttgaa tgagcgggtg gcagtattga aatcgcctga cagggttaaa
     2581 tttcgaacct cttgtatgtc ggggtaaaat atggccatta atctgtccgc attttttata
     2641 gaaactgtat aattcgcctt ttgtacttcg agggtggaat cgatgcgacc atttgtataa
     2701 taatctacta ggcttttaat tgattcatcg agtttgcttg gaaggaaatt cccctctaat
     2761 tgtaaatcga tgagagtaga attagccttg atgctttttt tatcgccaat ttgagctgca
     2821 gtaaaattta atgtgtctaa gcgataaata tatttatcgc gaataagtaa cagatctccg
     2881 aagtgcaaat tgccttcaat attgtcgagt gaagttccac cggcatctgc agaagctgtt
     2941 gcgctgaaaa ctattggttc gggatagaaa tttaattgct tcaagtttgc acgggcaata
     3001 tttgctttaa aatcatagag tggaatgctg tcctgcatat tgatgatacc attgaatgtt
     3061 agttttacat tcggatcatc aatagtgaaa tttccgctga aataatcatt ttcaagattg
     3121 ccatcaacca ccatattgct ataggtgtaa tcattaaatt ccatcatact gatttctgat
     3181 tgcatgtcca tttccatttc attatcacga gcaatggatc cgtcgatggt ggcatgcatg
     3241 ctgattcttc ccaacaaatc gggttcgccg gcaataatgc ctacattaaa tgcagaggta
     3301 ttcagatcgc cgcggaatga tgactttccg agatcgggat catattgaaa atgtacatcg
     3361 gtgtacatat ttccctgatc gctggtgagg tttcccagcg tcacaaggtc ataggcaaat
     3421 ccggtaacgc gaccggtgta attgatggtg ccaagcgcaa gcaagttatc cgggatagca
     3481 cctgctccga ttatattttc aagtcgatag gtatttacat atagtggttc caattccata
     3541 tcgataaatg tttcatcgat attcggcagg ccttttacat ttaatgtgcc acgcaatccg
     3601 gcagattcgt ttaacatggc atcgaaatct cgacccttaa gattgtcaag tgctccgtag
     3661 atatgtccgc tcagcaataa atgtgcatcg tatttatcta aaacgggtgc gaaatatgct
     3721 aaatctttat tcccgacaat tgttttatcg aaattgccat ccatgcgcac atcagattcg
     3781 aagcgatcaa aatcgttcag ggtagtgaac gtcatcgcga agcttttctg aattttgctg
     3841 ttcggtgttt taattaatag atctgtaagt gaaatttcgt atggcgagaa caacacattc
     3901 gcatgtaaag tgtcaacacg gaatcctgat ttttcggtaa gtgcaagatg atcgatgcga
     3961 ttcatgatag tgtcgccatg ataaatgata tccgtcattt caaggtcgat cccggtgatg
     4021 tccatgtcgc tgaaattaat tccgcgattt ggcgttgtgt cattcacatc tagaaatgca
     4081 aatgcgcaat tatgcaattt cagttgagag gcttccatca cccagtcgcc catggcaata
     4141 tgcacggtat cccagctttc cggtgtattg cttggaatgg tatctgttag tcctgtcaat
     4201 gcaaaccgcg tatcatcaat ctgcaatttt tccaaagcaa taatgtgatc ttcgaggtca
     4261 agggtgttga tgagcagttc taaatcgcca acatgcattt gcatatctga agcagcaact
     4321 tcatcatgca aaacaaaatg cacgttttta agatccagtc tgtttaattt cagttgaaca
     4381 gatcttcctc caccggatga tgatgtgctc gtatccttat tgtcattgct cgaaaatgcc
     4441 tctgaaatga aatcgaaatt ataaattgag tcgggcaagg ttctatcgag ataaacacgt
     4501 gtatcgtata atccgatact tttgattttc acataaggta caagtgggtt gatggtaccg
     4561 atattcactt ccagtttccc ggcatagagt agggtgtcgt cttgtttatc ttctatatat
     4621 aatccttcca gaataagatg atcgagcaga tcaatactca cttctttcac actcacttcg
     4681 gtatcgagtt tttcactcag ccatgaagtg gttttttccg ctacccaatt ttgaacggca
     4741 gggatgcgaa gcaacaggaa aatggtaaga aacagcaaca gtaccgtggt cacgaagatg
     4801 accgtgatac gccgaaatat cgaataaacc ctccaatatg tgaacagttg ctgcactaaa
     4861 gccttccggg tgttattttt acgctgttaa gttacgaata tcctatgcct gtgacaattt
     4921 tagggatcga gtcgagttgc gatgaaactg ctgctgctgt gcttatcaac ggacagatca
     4981 tgtccaatgt gatagcaggg caatcggttc atgagcaata cggtggtgtt gtgccggagc
     5041 tcgcctcccg tgctcatcaa gcaaatatcg ttcctgttgt gactgtggcg cttagtcgcg
     5101 cgggcatttc actcgccgac cttgatgccg tggcttttac acagggaccc ggtttgttgg
     5161 gtgcactcat ggtaggcacg gcatatgcca aagcactggc gatttcattg gacataccgt
     5221 tgataacggt tcaccatatg caggcacata ttttggccca tttgatagaa gatcccaagc
     5281 cggcatttcc ttttctttgt ctgacggtaa gtggcgggca tacgcagatc gttagggtga
     5341 acagtgccat ggat
//
LOCUS       DAOQOU010000197         5320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9423_length_5320_cov_5.479582, whole genome shotgun sequence.
ACCESSION   DAOQOU010000197 DAOQOU010000000
VERSION     DAOQOU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_5320_cov_5.479582"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="PLM90_12940"
     CDS             <1..1658
                     /locus_tag="PLM90_12940"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HMZ90335.1"
                     /translation="DEIVKMSLHRKAESAKTVIGFIKDQIDSIEVQLFNQETLLKNFK
                     QNNQLISPEIAETNIVEKLNEIDKAKFEVMLEEKSLGWLRDYVNNKNNDLEAIGNYFG
                     DMQYNDFSPYINSLITLQREKEDLGLSVQSNDPRIISINKQIEQVKVNFKSAIDNGQK
                     KLDVRKNYLAQEQDKYESMFYALPEKQGEYARLTRLNDLKEKYYILLLEKQSEYEITL
                     AGMTSDYIILDSAEKGSLVAPVKVKVWGLALLICLGISFAHVYVRFLTHSVIIGVPDV
                     ENATNVPMLGVLPQLAKQKTELPKVVVSSMPKSHIAEAFRSLRSNMQYFLKSDKKSHL
                     ITITSTVSGEGKTFVALNLTNVISATGKKVILVDFDLRKPKVHKAFNWRNDKGTSTIL
                     IKKHQPEDCIQFNEEIGCDILIAGPVPPNPAELLISPAANDLISYLKEKYDYVIIDNP
                     PVGIVSDSIPILGLADLAIYIIRADYSKRNFVNNVNRLYNDNKLKNLSIIVNDADASG
                     TEYGYGYGYGYGYGYSGYNDYYTDNIRHKSFFRRFFSSKRKRK"
     gene            complement(1715..4543)
                     /gene="uvrA"
                     /locus_tag="PLM90_12945"
     CDS             complement(1715..4543)
                     /gene="uvrA"
                     /locus_tag="PLM90_12945"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMZ90336.1"
                     /translation="MAMLDVQGARVHNLQNIDISIPRNSLVVITGVSGSGKSSLAFDT
                     IYAEGQRRYMESFSSYARQFFGGIERPDVDNITGLSPVISIEQKTTNKNPRSTVGTVT
                     EIYDFMRLLYARASDAYSSVTGKKMVRFTEEQIIQSIIKKFDGKRIVLLAPVVRGRKG
                     HYRELFEQIRKQGYLKVRVDGEVKDLLPKMQVDRYKVHDIEIVIDRFKVDEDDMVRIK
                     QSVAQALKAGKQFIMVMEVDGKGIFNYSKKLMCLDSGISYEEPSPNTFSFNSPYGACP
                     KCKGLGVVYEVNVDTLIPDKSKTINNGGILALGEYRDNTLFKQIKSLSGKYKFTLSTP
                     IKDIPKKGLNAILFGSEEDADVDVDDSVFLGENDRWYTLEYGGLAGLIKRCFSNSISE
                     NMRRWAEDFMEKATCDACNGTRLKEEALYFKVNNRNISELAQMDIKDLQLWFTDVEKG
                     MTEKNQIIAKDILKEIRARIGFIKNVGLDYLSLDRPTRTLSGGESQRIRLATQIGSQL
                     VGITYILDEPSIGLHQRDNNKLIAALKELKEGGNTVLVVEHDKDMMLAADHIIDLGPG
                     AGIHGGKLVAAGNPEQFMRAHTVTADYLSGVRSIPVPKTRRKGNGKHITLHGATGHNL
                     KNLTVKFPLGKLICVTGVSGSGKSSLINGTLFPLMQQHYYNSLKKPLPYKKIEGLDHI
                     DKVIEIDQSPIGRTPRSNPVTYIDVFSEIRNLFAIVPEAKIRGYKAGRFSFNVKGGRC
                     EVCEGGGMKVIEMNFLPDVYVECEKCRGRRYNRETLEIYFKGKNIADVLDLTVDHAVD
                     FFEHHPRIYKKIKTLQEVGLGYIKLGQASTTLSGGEAQRVKLAEELSRKDTGNTFYIL
                     DEPTTGLHFEDIRVLMQVITDLVDKGNTVLIIEHNLDVVKLADHIIDLGPEGGAGGGQ
                     ILVEGVPEEVIKHKSSHTGKFLRSELGL"
     gene            complement(4686..5105)
                     /locus_tag="PLM90_12950"
     CDS             complement(4686..5105)
                     /locus_tag="PLM90_12950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMZ90337.1"
                     /translation="MSDPIHTNKAPEPVGLYPHARRVGNLLFLSGIGPRIPGSNAIPG
                     LEQDKNGNFTAFDFEAQCRSVFDNVRIVLEASGATWNDLVDVTVFLTDMQRDFATYNR
                     IYAEYFKDAQPCRTTVGITSLPTPIAIELKCIAAIRE"
BASE COUNT         1584 a         1230 c          955 g         1551 t
ORIGIN      
        1 ccgatgagat tgtgaaaatg agtctgcatc gaaaagcaga aagcgctaaa acagtaattg
       61 ggtttataaa ggatcagatc gactccatag aagttcaatt atttaatcag gaaacactcc
      121 tgaaaaattt caagcaaaac aatcagctta tcagccccga aattgctgaa acaaatattg
      181 tagagaagct caatgaaatt gacaaagcca agttcgaggt catgctggaa gaaaaaagcc
      241 ttggctggct gcgcgattat gtgaataata aaaataatga tctggaagcc atcggcaact
      301 attttggcga tatgcaatac aatgattttt cgccttacat aaactcactc attacactac
      361 aaagagaaaa agaagatctt ggtctttccg ttcagtcaaa cgatccgcgc attatttcca
      421 ttaacaagca gatagaacag gtcaaggtca attttaaatc ggcaattgat aacggacaaa
      481 agaaactcga cgttcgtaaa aattacctgg cacaagaaca agataaatac gaatctatgt
      541 tttatgcctt acctgaaaag caaggagaat atgccaggtt gaccaggctg aacgacctaa
      601 aggaaaaata ttatatcctg ctgctggaaa aacaaagtga atatgaaatc acattggccg
      661 gtatgacaag tgattacatt attctggata gcgcagaaaa aggatcatta gtagcacctg
      721 tgaaagtaaa agtatggggt ttggcactat tgatttgcct cggtataagt tttgctcatg
      781 tatatgtcag gttccttaca catagtgtaa ttatcggtgt gcctgatgtt gaaaatgcca
      841 ccaatgtgcc aatgctgggt gtgcttccgc agttagcaaa acaaaaaact gagctgccca
      901 aagttgttgt atcatccatg ccgaaatcgc atattgctga agccttccga tcgcttcgca
      961 gtaatatgca atatttcctt aagtcagata aaaaatcgca tctgattacc attacgtcta
     1021 ctgtcagtgg tgaagggaaa actttcgttg cactgaactt gaccaatgta atcagcgcca
     1081 ccggtaaaaa ggtaattctg gtcgatttcg atttgcgcaa gccaaaagtg cataaagcat
     1141 ttaattggag gaatgacaaa ggcacaagta ccattctcat aaaaaaacac caacccgaag
     1201 actgtattca attcaatgaa gaaatcggat gcgatatcct cattgcaggt cccgtgccgc
     1261 caaatcctgc agaattactc atatcacctg ctgcaaatga cctcatcagt tatttaaaag
     1321 aaaaatacga ctacgtaatc atcgataacc cacctgtagg tattgtttcc gattcaattc
     1381 caattttagg ccttgccgat ttagctattt acattatccg cgctgattac tcaaaacgca
     1441 atttcgtgaa caatgtgaat cgcttgtata acgacaacaa actgaaaaac ctcagtatca
     1501 ttgtcaatga tgcggatgcc agtggaacag aatatggcta tggttatggc tacggatatg
     1561 gttatggata ctcaggatat aacgactact acaccgataa tatcaggcat aagtcattct
     1621 ttagaagatt cttttcttct aagaggaaga ggaaataact tattctgaat taatatctat
     1681 gtgcaaagaa ctaccttctt cgcacttaat caaatcacaa acccaattct gatcgcagaa
     1741 attttcctgt atggctactt ttgtgcttga tcacttcttc aggaacacct tcaactaata
     1801 tttgcccacc gccggcgcca ccttcaggac caaggtcaat tatatgatcg gctaatttta
     1861 ccacatcgag attgtgctca atgatcaata ctgtatttcc cttatctaca aggtcagtga
     1921 tcacctgcat caacacacga atatcttcaa agtgtaatcc cgttgtaggt tcatctaaaa
     1981 tgtagaaagt atttccggtg tctttgcgcg ataactcttc cgccagcttc acacgctgcg
     2041 cttctccacc gcttaatgta gtagatgctt gcccgagttt aatatatcca agaccaacct
     2101 cctgaagcgt cttaatcttc ttgtaaatac gcggatgatg ttcgaaaaaa tctaccgcgt
     2161 gatcaacggt caaatccaat acatcggcaa tattttttcc tttgaagtaa atctccaagg
     2221 tctctcgatt ataccttctt cctcggcact tttcacattc cacatacaca tcaggaagga
     2281 aattcatttc gataaccttc attcccccac cttcacaaac ttcacatctt ccacccttca
     2341 cgttgaatga gaatctaccg gccttataac ctctgatttt tgcttcagga actatagcaa
     2401 acagattacg aatctccgaa aacacatcaa tataagttac cggattcgaa cgcggtgttc
     2461 tgccaattgg actttgatca atttcaatta ccttatctat atgatcaagt ccttctatct
     2521 ttttatatgg aagtggcttc tttaacgaat tataataatg ctgctgcatc aatggaaata
     2581 atgtgccatt gatcaaactg gattttccgc ttccgctgac accagttaca caaattaatt
     2641 tccccaacgg aaatttcact gtcaggttct tcaaattatg acctgtagct ccgtgcaaag
     2701 taatatgttt cccgttaccc ttcctgcgtg ttttaggaac cgggatactc cttacaccac
     2761 tcaaataatc agccgtaaca gtgtgcgcac gcatgaactg ttccggatta cccgcagcta
     2821 ccaacttgcc accatgaata ccggcacccg gaccgagatc gataatatga tctgctgcca
     2881 acatcatatc cttatcatgt tccacaacaa gcacagtatt accgccctct ttcaattcct
     2941 tgagtgctgc aatcaattta ttattatcac gctgatgcaa accaatcgac ggctcgtcta
     3001 aaatgtaggt gatacctacc aattgcgaac cgatctgcgt tgccaaacga atacgttgcg
     3061 attcaccgcc actaagtgtt ctcgttggcc tgtcgagact caaataatca agcccgacat
     3121 tttttataaa acctattcga gcgcgaattt ctttcaatat gtctttcgca atgatctgat
     3181 tcttttcagt catgcctttt tcaacatccg taaaccacaa ttggaggtct ttaatatcca
     3241 tttgcgctaa ttcagagatg ttgcgattat tcaccttaaa atacaaggct tcttccttca
     3301 aacgggtacc attacaagca tcgcaagtag ctttctccat aaaatcctca gcccagcgac
     3361 gcatgttttc ggaaatcgaa tttgaaaaac aacgcttgat caatcccgct aaaccaccat
     3421 attccaatgt ataccagcga tcattctccc caaggaaaac gctatcatca acatctacgt
     3481 cagcatcttc ttcacttccg aataaaatgg cattcaaacc ctttttagga atatctttta
     3541 ttggtgtgct gagcgtgaat ttatatttac ccgataaaga tttaatttgt ttaaataagg
     3601 tattgtcgcg atattctcct aatgccagaa taccaccatt gttaattgtt ttagacttat
     3661 caggaataag cgtatctaca ttcacttcat aaacaacacc cagccctttg catttcgggc
     3721 atgctccgta aggtgagttg aaagaaaatg tatttggcga tggttcttca taactgatac
     3781 cgctatcaag acacatcaat tttttagaat aattaaaaat tcctttaccg tcaacttcca
     3841 tcaccatgat gaattgcttt ccggccttga gcgcctgggc aacgctttgc ttgatgcgca
     3901 ccatatcgtc ttcatcaacc ttaaatctat caatcacaat ctcgatatcg tgcaccttgt
     3961 acctgtcgac ttgcattttc gggagcaggt ctttcacttc tccgtcaact cgtactttta
     4021 aatatccttg cttgcgaatt tgctcaaata attcgcgata atgacctttt cttccacgca
     4081 ctacaggagc caacaacaca atgcgcttgc catcaaattt cttgataatg ctttgtataa
     4141 tttgctcctc cgtaaatcgc accatttttt ttcctgtcac cgaagaataa gcatccgaag
     4201 cgcgcgcata caacaagcgc atgaaatcat atatctctgt aacagttccg acagttgaac
     4261 gcggattttt attcgtcgtt ttttgttcga tagaaattac cggcgacaat ccggtgatat
     4321 tatctacatc aggtcgctct atcccaccaa aaaattgtct ggcataggat gagaaacttt
     4381 ccatatatct gcgctggcct tcggcataaa tggtatcaaa tgccaatgag ctttttccac
     4441 ttccgctcac cccggtgatc accaccaagc tgttccgcgg aatggaaata tcgatattct
     4501 gcaaattgtg cacgcgggct ccctgcacat cgagcatagc catttcactt tcactgatag
     4561 ccggaagcga aatagatgtc tgagctatat tttttgttga ttttgccacg cgtcagtaac
     4621 acgaaattcg tgcaaaggta agggatttga aagcgaaaaa acagcaatta cctatgtttc
     4681 gttcactact cccgaatagc ggctatgcat ttcaattcaa tggcaatggg tgtaggcagc
     4741 gatgtgattc ccactgttgt gcggcatggc tgtgcatcct tgaagtactc ggcataaatg
     4801 cggttgtagg tggcaaaatc gcgttgcata tcggtgagga atactgttac atccaccagg
     4861 tcgttccagg tagctcctga agcctctaat actatgcgca cattgtcgaa taccgaacgg
     4921 cactgcgcct caaaatcgaa ggcggtgaaa ttgccgtttt tatcctgttc caagcccgga
     4981 atagcatttg aacccggaat acgtggtcct attccactca aaaacaacaa attcccaact
     5041 cttctagcat gtggatataa gcctaccggt tcaggggctt tattggtgtg gatcggatct
     5101 gacattgcct gcaatttaag gccgaaatta cggtttatac ttgtcgaaag taacaaaata
     5161 ctctacttat tcgttttatt gatattccga ttgcctaaac cggtacaacc aacctctttc
     5221 gcgcaatccg gacattcgcg tttcactatt aaaccaaacc atatgagaag tttgtttaca
     5281 ctgcttcttg cttgctctgc gatttttatt tcctgcgaca
//
LOCUS       DAOQOU010000198         5300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9472_length_5300_cov_5.559390, whole genome shotgun sequence.
ACCESSION   DAOQOU010000198 DAOQOU010000000
VERSION     DAOQOU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9472_length_5300_cov_5.559390"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1835)
                     /locus_tag="PLM90_12955"
     CDS             complement(102..1835)
                     /locus_tag="PLM90_12955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ90338.1"
                     /translation="MNKFAELGLSSRIVEGIEVLGFTEPTPIQAQVIPAVLNEPTDLI
                     ALAQTGTGKTAAFGLPLVEMIDVTARIPLALILAPTRELCVQIENDLRNFSKTTKGFQ
                     TVAVYGGASIRTQIEQIRRGVQVVVATPGRLIDLITRKALNLSDVQVVVLDEADEMLN
                     MGFMEDIDTILAETPEDKQTWLFSATMPTEIRQIIKKYMSNPVEISSGGGNKTNENIE
                     HQYYMVEARNKYPALKRILDFHPDIYGIIFCRTKAETQDMAERLIKDGYNADSLHGDL
                     TQMQRDKVMNAFREKTLQVLIATDVAARGIDVDNLTHVLHLHLPDDMAYYTHRAGRTA
                     RAGKTGVSCVLVTSKDLYRIKQLERTLKIDFKKMMVPDGMAICEKQLLNLLHRVNEVK
                     VDSAAIARFMPVMEREFAGMDRDEIMMRFASLEFNRFLDYYRFAEDLNVYERRETRDQ
                     AARKGNMDLLFINLGKMDHIGPKDLLVMICQVGKLKAHQIGDIRLKGAYSFIEVPANA
                     SQTIIQAFNGFIYRGRRVRLEIRNDKEESFSEKRRSHKGSSREMPRKEHGSGFKKDGG
                     FKKEHRKGKKG"
     gene            2079..4199
                     /locus_tag="PLM90_12960"
     CDS             2079..4199
                     /locus_tag="PLM90_12960"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ90339.1"
                     /translation="MRKFILLLIVLGFVGFQAQAQVNRCGVMEHEAVLQAKYPDYATK
                     QAAREQMTQKIIQKMQANGKMEGGATIVTIPVVFHVVYKTAAQNIPDARLLEQIDILN
                     EDFRRLNDDAANTPAAFTGVAADFEIEFCLASIDPNGNPTTGINRIETTVSSFGTDDN
                     VKFAADGGANAWPADSYLNFWVCNLGASLLGYAQFPGGPANTDGVVIHYNHVGNNAAG
                     YPYHLGRTATHEIGHWLDLRHIWGDDTNCAGSDLCGDTPNQKVETYGCPGYPKTDACS
                     ATSPGIMFMNYMDYTDDDCMNMFTEDQKSRARALFEPGGDRYGLLFSEGCGLQPFDAQ
                     AISSLPGGTVCNLSFTPAVEIKNHGTETLTSIDIIYSVDGGATSTFGWTGSIVTNATE
                     LVYLPSLTTTEGEHTLEVTLENPNGNTDADASDNITSTDFLVNLGSSMLPMVQGFEAS
                     GFPYTDYTLYNPDGGEGWERTNFGASIGTYSIFLNHFNNSNFGDIDEFVLPAYDMSDL
                     DEVHFTFDVAYALYTASGVYSDTLEVLVSDDCGDSWTTLYKKANPDLQTAPPHTITFK
                     PEDDEWRQESIDLSAYLGQPQVFVKFRTITNYENNLYVDNININDGTLPQAIDDLAND
                     FALSLFPNPTSNNITVRFNTPIAGAYSVIVYNVTGALVYTSNTNASNGVNLISVPAEG
                     LGAGLYHVQVSNGVLVSGDYFIKE"
     gene            complement(4246..4908)
                     /locus_tag="PLM90_12965"
     CDS             complement(4246..4908)
                     /locus_tag="PLM90_12965"
                     /inference="COORDINATES: protein motif:HMM:NF036823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HMZ90340.1"
                     /translation="MALILREHYQHLAEYAVWKIEEEPEFYLKGLILSEWEQNYLNRI
                     NHPKRRITWLASRYLLKLLLNTSAFVELLFDEHGKPYVANDIVKVSISHCDTYAAAMV
                     SRECGVGIDIETPERDISPIQNKFLSPTELEQINTGDKRELLMVYWGAKEVIYKIYGK
                     RKLEFKDDMYIKPFILQQRGDIDGMLMKNGLVADYLMHYFQNDRFILVVGVEKEVSIL
                     NQ"
     gene            4971..5234
                     /locus_tag="PLM90_12970"
     CDS             4971..5234
                     /locus_tag="PLM90_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90341.1"
                     /translation="MHATRSIFGMQNHTMFKFFQFVRAEQEFLHIEVPKHLKGKLVEI
                     ILQEKAPDPDTQLAPDEEQQMAQKEAELKMLDDMRNQLNVWDT"
BASE COUNT         1404 a         1241 c         1190 g         1465 t
ORIGIN      
        1 cttcaaactt gctcactaat acgagcgtct tcatgcaacc cccccccatc ggctaattgg
       61 ctaatcggct aattggctaa ttggctaatt agctaatcac ctcatccctt cttccccttc
      121 ctatgttcct tcttaaaccc gccgtctttt ttgaaaccgg agccatgctc ttttcggggc
      181 atttcacggg acgaaccctt gtgtgaacgg cgtttttcac tgaagctttc ttctttatcg
      241 ttgcggatct caagtcttac acggcgacca cgataaataa atccgttgaa agcctgaata
      301 atggtttgag atgcattagc agggacttca atgaatgaat atgcaccttt caggcggata
      361 tcaccaattt ggtgggcttt gagcttgcca acctgacaaa tcataaccaa caagtctttc
      421 gggccgatat gatccatttt acccaggttg ataaacaaca agtccatatt gcccttgcga
      481 gcagcctgat cgcgggtttc gcggcgttcg taaacgttca ggtcttcagc aaagcggtaa
      541 taatcaagga aacggttgaa ttctagactg gcaaaccgca tcatgatttc atcgcggtcc
      601 attcccgcaa actcacgctc catcaccggc atgaatctgg ctattgctgc tgagtctacc
      661 ttaacttcgt ttaccctgtg caacaggttt aggagctgtt tttcgcagat agccatgccg
      721 tctggcacca tcatcttctt aaagtcgatt ttcagggtgc gttcaagttg ctttatgcga
      781 tagaggtctt tagaagtaac caacacgcaa gaaacccctg ttttcccggc ccttgccgtt
      841 cttccggcac gatgagtgta atatgccata tcgtcgggga gatgcaggtg cagcacgtgt
      901 gtcagattat ccacatcaat acctctggca gcaacatccg tagctatgag cacctgaagt
      961 gtcttttcac ggaaggcgtt catcacctta tcgcgttgca tttgggtaag gtcgccatgc
     1021 aaagaatcgg cattataacc gtccttgata aggcgttcgg ccatgtcttg cgtttctgct
     1081 ttcgtgcgac aaaaaatgat tccgtaaatg tcgggatgaa aatcgaggat gcgttttaag
     1141 gcaggatatt tattcctcgc ctcaaccata taatattgat gctcaatgtt ttcgttagtc
     1201 ttatttccac caccggatga gatctctacc ggattgctca tgtatttctt gatgatctgg
     1261 cggatctctg taggcatggt tgcagagaac aaccaggtct gcttgtcttc gggagtttcg
     1321 gcaagaatgg tatcaatatc ttccatgaaa cccatattca acatctcatc agcctcatca
     1381 agcactacaa cttgcacatc gctgaggttg agcgccttgc gggtgatgag gtcgatcaat
     1441 cggcctggag ttgccactac cacctgcaca ccgcgtcgaa tctgctcgat ttgtgttctg
     1501 atacttgcgc cgccatacac tgcaacagtc tgaaaacctt tggttgtttt agagaagttg
     1561 cgcaggtcgt tttcaatctg cacacacaat tcgcgtgtag gtgccaaaat gagcgccaac
     1621 ggaatgcggg cggtaacatc tatcatttct accaatggta atccaaaggc agcagtttta
     1681 cctgtgccag tttgtgccag ggcgataagg tctgttggtt catttaacac ggcggggatc
     1741 acctgtgcct gtatgggagt aggttcggta aatcccaata cctctatccc ttccacaatg
     1801 cgtgatgaaa ggcctaattc ggcaaattta ttcatataat ttcttgtaat tcaaatattt
     1861 atatactgtt atctactaca ccacattatg cctgttcaaa gcgctcgtgg ggctaatttt
     1921 gaaaaatacc gtgagcagat acaaggagga atgcgctgca aagatactga aaatagcgct
     1981 gtttcagcta ttcgccaagt tgtggaagga aagtgcgtaa aacggcgtaa acaacaagca
     2041 ggaaaccctt acctttaagg cttattctaa tctacattat gcgtaagttt attttactcc
     2101 ttatcgtgct gggttttgtt ggctttcagg ctcaggcaca agtaaatcgt tgcggggtta
     2161 tggaacacga agcagtgtta caggctaaat accctgatta tgcgacaaaa caggctgcca
     2221 gagaacagat gacccaaaag atcatccaga agatgcaggc taatggcaaa atggaaggtg
     2281 gtgctactat agtgaccatt ccggtggtat ttcatgttgt ttacaaaact gccgctcaga
     2341 acattccgga tgcacgactt ttggaacaaa ttgacattct aaatgaggat ttccgtcgct
     2401 taaatgatga tgcagccaat acaccggcag cctttacagg agtcgctgcc gattttgaaa
     2461 ttgaattttg cctcgcttcc atagatccaa atggcaatcc caccacggga ataaacagga
     2521 ttgaaactac tgtgtcttca ttcggcacag acgataatgt gaagtttgct gctgatggtg
     2581 gagcaaatgc atggcctgcc gacagctatt tgaacttctg ggtgtgcaac cttggtgctt
     2641 cgttgttggg ttatgcacaa tttccgggag gaccggccaa cactgatggt gtggtaatac
     2701 attacaatca cgttggaaac aatgctgcgg gttatccata ccatttaggt cgcaccgcta
     2761 ctcacgaaat tggccactgg cttgaccttc gccatatctg gggtgatgat acaaactgcg
     2821 ctggatctga tctttgcggt gatacgccaa atcaaaaggt agaaacttac ggttgtccgg
     2881 gttaccctaa aaccgatgct tgttctgcta cttcacccgg catcatgttc atgaactaca
     2941 tggactatac agatgatgat tgtatgaata tgttcactga agaccaaaaa tcacgcgccc
     3001 gcgcattatt tgaaccgggt ggcgatcgct acggattgct gttttcagaa ggttgtggct
     3061 tgcagccatt cgatgcacag gccatttctt cgcttccggg cggaaccgtt tgtaacttat
     3121 catttacgcc tgcagtcgaa attaagaacc atggcacaga aacactcaca tcaatagata
     3181 tcatttatag cgttgacggc ggtgctacct caacattcgg ctggacagga tccatcgtaa
     3241 ccaatgctac tgaattggtg tatttgcctt cgttaaccac tactgagggc gaacataccc
     3301 ttgaggtaac gcttgaaaat ccaaacggca atactgatgc tgacgccagt gataatatta
     3361 cctctacgga tttcctggtt aacctgggat cttccatgtt acctatggtg cagggatttg
     3421 aagcttcagg cttcccttat accgactata ccctttacaa ccccgacggt ggtgaaggat
     3481 gggaaagaac gaatttcggc gccagtattg gcacatattc catattcctg aaccacttca
     3541 ataactccaa ttttggtgat atcgatgagt ttgtacttcc ggcttacgat atgagcgatt
     3601 tggatgaagt gcatttcacc tttgacgtag cctatgcgct gtatacagcc agcggcgtat
     3661 actccgacac cctggaggta ttagtaagcg acgactgcgg cgattcttgg actacacttt
     3721 ataagaaagc caatcctgat ttgcaaacag caccacctca taccataaca ttcaaaccgg
     3781 aggatgacga gtggcgtcag gaatcaattg acctgagtgc atatctcggt cagcctcagg
     3841 tgtttgtgaa attccgcact atcacaaatt acgagaataa cttgtacgtg gataatatca
     3901 atatcaatga tggcacgtta cctcaggcta ttgacgattt ggccaacgat ttcgctttga
     3961 gtctcttccc aaatccaacg tcaaacaata taacggtgcg tttcaacacc ccgattgcag
     4021 gggcttacag cgtgattgtg tacaatgtta cgggagcttt ggtttataca tcaaacacaa
     4081 atgcttctaa cggtgtaaac ctgatcagcg ttcctgccga aggtcttgga gcaggcttgt
     4141 accatgtgca ggtaagcaat ggcgtcctcg tttcaggtga ttatttcata aaagaataag
     4201 caaaccttaa ccaatttata gaaggcctcc gatcgggggc cttttttatt gattgaggat
     4261 ggagacttcc ttctcaacgc ctacaaccag aatgaatctg tcattttgga agtagtgcat
     4321 caggtaatca gcaaccagtc cgtttttcat aagcattcca tcaatatcgc ccctctgttg
     4381 cagtatgaaa ggcttgatgt acatatcgtc cttgaattcc agtttccgct taccatagat
     4441 cttataaatc acttccttgg cgccccaata aaccatgaga agctctctct tatcgccggt
     4501 attgatctgt tccagctcgg ttggcgataa gaatttgttt tggatgggtg aaatgtcgcg
     4561 ctcgggcgtt tcaatatcga tgccaacacc acactccctc gacaccatag ctgcggcata
     4621 ggtatcgcag tgactgatac tcacttttac gatatcgttg gcaacgtaag gtttaccgtg
     4681 ttcatcgaaa agtagttcaa caaaggcgga ggtattcaac agaagcttaa gcagatagcg
     4741 gcttgcaagc caggtaatac ggcgctttgg gtggttgatg cggttgagat agttttgttc
     4801 ccattcgctg agtatgaggc cctttaagta gaattcaggc tcctcctcta tcttccagac
     4861 agcatattcg gccagatgct gataatgttc ccgcaaaata agtgccatgc taaactattc
     4921 acaggcgaaa ttaggctaat taaacctcgg cagacaaaaa atatttgcac atgcatgcaa
     4981 cccgcagtat cttcgggatg caaaaccaca ccatgtttaa atttttccag tttgttcgcg
     5041 ccgagcagga attcctgcat atagaggttc ccaagcacct gaaaggcaag ctggtagaga
     5101 ttattctgca agaaaaagcc cccgatcccg acacccaact cgcccccgac gaagaacagc
     5161 aaatggcgca aaaagaagca gaactcaaaa tgcttgacga tatgcgcaat cagttgaatg
     5221 tttgggatac gtgaggtgat gagttgatta gccaattagc agattagcag attagccaat
     5281 tagccgattg ggagagtgct
//
LOCUS       DAOQOU010000199         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9548_length_5274_cov_5.666411, whole genome shotgun sequence.
ACCESSION   DAOQOU010000199 DAOQOU010000000
VERSION     DAOQOU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9548_length_5274_cov_5.666411"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLM90_12975"
     CDS             complement(<1..425)
                     /locus_tag="PLM90_12975"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HMZ90342.1"
                     /translation="MSKTSSNTGFLNIYVLIGASHIIALALHVDIVVMLTKFLLMPAL
                     MLHVYKHVGVSTVLRNRLLFVLFFSWMGDILLMFDENNSNFFIFGLAAFLIAQAAYIT
                     AFSIAAHKSNGSSLPPGLLLIPPIYAGILLSILIPHLGN"
     gene            complement(406..1659)
                     /locus_tag="PLM90_12980"
     CDS             complement(406..1659)
                     /locus_tag="PLM90_12980"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ90343.1"
                     /translation="MPEAVNYIVYSIPVFFLLIGVEILIDKARKTGYYRLNDALSNIN
                     AGITEQVTGAFLKTFVVGLYAWTYDHMRIASIPDNIVMWIILFIGVDFFYYWFHRLAH
                     EINVLWGGHVVHHQSEEYNLSVALRQGAFQKFGSFLFYLPLALIGFNPVMFIVVSQFQ
                     TLYQFWIHTKVVNKLPAPFEFILNTPSHHRVHHGRNPKYIDKNHGGTLIIWDRMFGTF
                     QDEEEEVIYGITKPLKTWNPLRAQLDFWRDLWHDLARTPGIGDKIKLLLLPPGWYPAV
                     LGGPQTPPSINKQTEKKFDTHISMAMNTYVFIQFVITLGITSWFLFTYETMTLSSGLI
                     VMAFVLISITATGLLLEGNRWAVFVERLRLIGLPLVFFYLHYIGFFGIIPVRYYNPLL
                     AYVVVSLIIFTFILRRNSLHVKNIK"
     gene            1716..3458
                     /gene="ggt"
                     /locus_tag="PLM90_12985"
     CDS             1716..3458
                     /gene="ggt"
                     /locus_tag="PLM90_12985"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HMZ90344.1"
                     /translation="MNCYLYTMRYSIFLVIWLWSCTSSTQPADKPVTDNPGVSLEGRT
                     HGVLGDSAMVVCAHPIAAQVGADILRKGGNAVDAAVAVQFALAVVYPNAGNIGGGGFM
                     VMRSQDGSTTALDFREKAPSASDRDMFINKQTGEVDRKKIETSHLASGVPGSVAGMWE
                     AHQKYGTLPWADLIQPAIDLAENGFPVTKNQAEDLRGLQRELKSLNPGKNYFRKNDWK
                     EGDLLIQQDLANTFIAIRDNGRDGFYSGEVAKKIVAEMSSHNGIISGDDLKNYQAVWR
                     TPVQGTYRGYTIISMPPPSSGGVALMQLLNMMEPFPLSDWGFHTTATMSIMVEAEKRV
                     YADRATWLGDPDFAKVPTTELTAKSYAAQRMLGVDTNTVTPSKNIKAGNIPGYESEET
                     THFSIVDQFGNAVAITTTLNDSYGSRIVVAGCGFILNNEMDDFSAKPGEPNLYGLIGG
                     EANAIAPNKRMLSSMTPTIVLKDNKLFMVIGSPGGSTIITSVFQNIVNVIDFNMTMQE
                     SVNASRFHHQWLPDEIKSETSMDEVVLQKLKAKGYTLSSREAIGRVDAILIHEGSLFE
                     GAPDPRGDDAAVGW"
     gene            complement(3746..4639)
                     /locus_tag="PLM90_12990"
     CDS             complement(3746..4639)
                     /locus_tag="PLM90_12990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ90345.1"
                     /translation="MHCVSCGHTHFSATPYYYQWNSKEFVLTTCKNCGLITMDPKPTD
                     EELGLLYADEYFETGQHGLNRIGMTYEQGQDKNGIDHRINAVKQNMLAFKPDMQSVFE
                     IGFAMGHMLAAARDLGLEIGGIEFSPMAVSRAKEKFDIDALCGNFENIDLSAHLHKWD
                     CVYGGDVFEHFIHPDLVVKNMHRILKDNGVAIVAVPSTFNLFSTHIATTIFKLMGKRK
                     RFVDKPYHVFEYTPRTISALFKKEFREVQVVNRIKKPSQLNMKTGGFEYKIKYLIHVI
                     NYPFTKLFNRNGDRLLVVARK"
     gene            4747..>5274
                     /locus_tag="PLM90_12995"
     CDS             4747..>5274
                     /locus_tag="PLM90_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HMZ90346.1"
                     /translation="MHSNTFYRTHTCGELRMQSVGTTVTLSGWMHRSRDLGGMTFIDL
                     RDRHGITQLVFNMESNAALCEMARKLGREFVISASGVVTERSSKNAQLPTGDIEIIVD
                     KLEILNAAELPPFTMDDETDGGEELRMRYRYLDLRRPQMQQRLMLRANMLKAVREYLD
                     AEGFVEVETPNLIKST"
BASE COUNT         1632 a         1128 c         1090 g         1424 t
ORIGIN      
        1 atattgccaa ggtgtgggat taaaatagat aacaatatac ctgcataaat tggtggaatc
       61 aataataatc ccggcggcag tgaacttcca ttagatttat gtgcagcgat ggaaaatgca
      121 gtgatgtatg cagcttgtgc gattagaaat gcagccagtc cgaagatgaa aaagttggaa
      181 ttattttcat caaacatcaa caaaatatca cccatccaag aaaagaataa tacaaacaac
      241 aatctgtttc tcagcactgt tgatacccct acgtgtttat atacatgcaa catcaatgcc
      301 ggcatcaata agaactttgt aagcatgaca acaatatcta catgcaaggc tagtgcaata
      361 atatgcgatg cgccaattaa tacatatata ttcaaaaatc cggtattact tgatgttttt
      421 gacatgcaaa gaatttcgtc gtaaaataaa tgtaaatatg attaaagaaa caaccacata
      481 ggccaacaat ggattataat acctgacagg aatgatgcca aagaagccga tataatgcaa
      541 ataaaaaaac accagaggca atcctattaa tcgaagtcgc tccacaaaca cagcccatct
      601 atttccttcc agaagtaacc cggttgcggt tatgctgatt aaaacaaaag ccatgacaat
      661 taggccactt gataatgtca ttgtttcata agtaaataga aaccatgacg ttatgcccaa
      721 tgtgatcaca aattgtatga acacataagt attcatcgcc attgaaatat gcgtatcaaa
      781 tttcttttct gtttgtttat ttatcgatgg tggtgtttgc ggtccaccta ataccgcagg
      841 gtaccagccg ggaggcagca acaacaattt gattttatct ccaataccgg gagtgcgcgc
      901 aagatcatgc caaaggtctc tccagaaatc gagttgcgcg cgaagcggat tccatgtttt
      961 gagtggtttt gtaatgccgt aaattacttc ttcctcctca tcttgaaatg taccaaacat
     1021 cctatcccag ataatcaaag tgccaccatg atttttatcg atatacttcg gatttctgcc
     1081 atgatgcaca cgatgatgcg atggcgtatt taaaataaat tcaaatggcg caggaagttt
     1141 attgaccacc ttggtatgaa tccaaaattg atataatgtt tgaaactgac tcaccacaat
     1201 aaacatcaca ggattgaaac ctataagcgc caatggcaga taaaataaaa atgacccgaa
     1261 tttttgaaat gctccttgac gcaaggcaac acttaaattg tattcctcac tttgatgatg
     1321 cactacatgt ccgccccaca acacattaat ttcatgtgca aggcgatgaa accaatagta
     1381 aaaaaaatct acgccaataa ataagatgat ccacatcaca atattatccg gaatactagc
     1441 gatgcgcata tgatcataag tccatgcata caatccaact acaaatgttt tcaaaaatgc
     1501 accggttacc tgctctgtta tgccggcatt gatattcgac aaagcatcat tcaaacgata
     1561 gtaacctgtt ttcctcgctt tatcgataag tatctccaca ccaatcaata aaaagaatac
     1621 cggaatggaa taaacaatat aatttactgc ttcgggcata caaaatgata ttgttcaaat
     1681 gtaatagttt tcaggtaacc ttggaggctc tgcttatgaa ctgctatctt tataccatgc
     1741 gatattccat tttcttagtt atctggttgt ggagctgcac ctcctctacg cagcctgccg
     1801 acaaacctgt taccgacaac cccggcgttt cactggaggg aaggacccac ggcgttttag
     1861 gcgattcagc catggtggtt tgcgcacatc caatcgcagc tcaggtaggc gcagatatct
     1921 tgcgcaaagg tggcaatgca gtggatgcgg ctgtagcagt tcaatttgca cttgcggtag
     1981 tatatcccaa tgccggcaat attggtggcg gcggatttat ggtgatgcgc agtcaggatg
     2041 gtagcacaac tgctctcgat ttcagagaaa aagcaccctc ggcatccgat cgcgatatgt
     2101 ttatcaataa acaaacagga gaagttgaca gaaaaaaaat agaaacatca cacctggcat
     2161 ccggcgtacc gggaagtgtg gcaggtatgt gggaagcaca tcaaaaatat ggaaccctcc
     2221 catgggctga tcttatccaa cctgctatcg acctcgcaga aaacggtttt cctgttacaa
     2281 aaaatcaggc agaggattta cgcggacttc aacgcgaatt aaaatcgcta aatccgggaa
     2341 aaaattattt ccgaaaaaat gattggaaag aaggcgactt actgattcaa caggatctgg
     2401 caaatacatt cattgccatt cgcgataatg gtcgcgatgg attttatagt ggcgaagtag
     2461 cgaaaaaaat tgtagcagaa atgtcatcac acaatggcat tatttccggc gatgatttga
     2521 aaaattatca ggctgtatgg cgcacaccgg ttcaaggtac ttatcgcggt tatacaatca
     2581 tttccatgcc tcctccaagt agcggtggtg ttgcattaat gcaattgctg aatatgatgg
     2641 aaccctttcc tttgagtgat tggggatttc atactaccgc aacaatgagt atcatggtgg
     2701 aagcagaaaa acgcgtttat gccgatcgcg ccacatggct cggagatccg gattttgcta
     2761 aagtgccaac aacagaactt actgcaaaat catacgctgc tcaacgcatg cttggtgttg
     2821 atacgaatac ggttacacca tcgaaaaata ttaaagcagg aaatattccg ggatatgaaa
     2881 gtgaggaaac aactcatttc agtatcgtag atcaattcgg aaatgcggtt gccatcacca
     2941 caactttaaa tgacagttat ggatcgagaa ttgttgttgc cggatgcgga tttattttaa
     3001 ataatgagat ggacgacttc agtgccaaac ccggagaacc aaatttatac ggactcattg
     3061 gcggagaagc aaatgccatc gccccaaaca aacgtatgct tagtagcatg acgccaacca
     3121 ttgtattgaa ggataataaa ttatttatgg tgataggatc tcccggaggc tcaacaatta
     3181 ttaccagtgt gtttcagaat atcgttaacg taattgattt caatatgacc atgcaggaaa
     3241 gtgtgaatgc ctcaagattt catcatcaat ggctgcctga cgaaatcaaa tctgaaacat
     3301 cgatggacga ggttgtattg caaaaattaa aagccaaagg atacaccctc tcctctcggg
     3361 aagccatcgg acgcgtagat gcaattttaa tacatgaagg ctcattattc gaaggcgctc
     3421 ccgatccacg tggtgatgat gctgccgttg gatggtgaaa attttaataa tgaaaatcga
     3481 tgctcaggct gtgcaataca agagtccaac aaatctgaca aagtgagtgg cagcttacaa
     3541 caaatatcgc aattaggcat ctgcggtgag ttgcgatttt caataatttg gttttgcctg
     3601 attacggaaa aacgccgctg ccatcgtgtt cggatgatgg tatccggcta agttcagggt
     3661 tttcagcact gacggctcct ccttacctga gatgcatccg tttcaactat gggcagcgat
     3721 gcagataagc aacaatggga attgatcact tcctcgcaac caccagcaac ctgtcgccat
     3781 tgcggttgaa caattttgta aacggataat tgatgacatg tatgaggtat ttgatcttgt
     3841 attcaaatcc tccggtcttc atatttaact gcgaaggctt cttaattctg ttcaccacct
     3901 gcacttcgcg aaactctttt ttaaatagcg cactgatggt tcgaggtgta tattcaaaca
     3961 catggtaggg cttatccaca aatcgcttgc gctttcccat cagtttaaaa attgtagtag
     4021 caatatgtgt agagaataaa ttaaaggttg atggcactgc cactattgca acgccattat
     4081 cttttaaaat gcgatgcata tttttcacca caagatcagg atgaataaaa tgttcgaata
     4141 catcgccacc gtacacacaa tcccatttat gtagatgtgc cgacaggtcg atgttctcaa
     4201 aattgccaca caatgcgtcg atgtcaaatt tttccttcgc gcggctcaca gccataggcg
     4261 aaaattctat tccaccaatt tcaagtccaa gatcacgtgc ggctgccagc atatgtccca
     4321 tagcaaatcc gatttcgaaa acgctttgca tgtcaggttt aaacgcgagc atattttgct
     4381 taactgcatt gatgcggtga tctatcccgt ttttatcctg accttgctca taggtcatgc
     4441 caatgcggtt taatccatgc tgaccggttt caaaatattc atcggcatac aacaatccca
     4501 gctcctcatc ggtgggtttg ggatccatgg tgatgagacc gcaattcttg catgtagtga
     4561 gtacaaattc cttactattc cattgataat aataaggggt ggcactgaaa tgagtatgtc
     4621 cgcaggaaac acaatgcatg atacgtagtg actatgtcgc tcaaaaatac gcaaatccgc
     4681 gtccttacaa gagtcagcgt ttttatccca ccccaccttt ccgtattttt gcagcaaatt
     4741 tcgaatatgc atagtaacac cttttatcgc acccatacct gtggagagct cagaatgcaa
     4801 tccgttggca ctacagttac cctttcggga tggatgcacc gtagccgcga ccttggaggt
     4861 atgaccttca tcgacctccg cgaccgtcac ggaatcacgc aattggtgtt caatatggaa
     4921 tcaaatgctg ctttatgcga aatggcgcgc aagctgggaa gagaatttgt gatcagcgcc
     4981 agcggagtag taactgaacg ttccagcaaa aatgcccaat tgccaacagg tgatatcgag
     5041 attattgtcg ataaactgga aattctcaat gcagctgagc ttcctccatt tactatggat
     5101 gatgaaacgg atggcggtga agaattgcgt atgcgctatc gttatctcga tttgcgtcgc
     5161 ccgcagatgc aacagcgatt gatgcttcgt gcaaatatgc tcaaagccgt tcgcgagtat
     5221 ttagatgctg aagggtttgt tgaagtagaa acaccgaacc tgataaaatc cact
//
LOCUS       DAOQOU010000200         5264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9578_length_5264_cov_5.082165, whole genome shotgun sequence.
ACCESSION   DAOQOU010000200 DAOQOU010000000
VERSION     DAOQOU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9578_length_5264_cov_5.082165"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PLM90_13000"
     CDS             <1..149
                     /locus_tag="PLM90_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HMZ90347.1"
                     /translation="FLIVAFVMFMVVKAMNSMKKKEEEAPAAPPAPPADVVLLTEIRD
                     LLKK"
     gene            243..1184
                     /locus_tag="PLM90_13005"
     CDS             243..1184
                     /locus_tag="PLM90_13005"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HMZ90348.1"
                     /translation="MKKMCTLIAAIAINAAVFAQTDSTSNWKKGGGAILNFNQTSLTN
                     WAAGGESNLSSTILTNFYANYKMGTTTLDNTLNMNYGLITANNYADIRKNEDRIELNS
                     KYGRYAFADDFYYSALVNFLTQFAPGYDYLADPDALVPISKIMSPGYLTAAAGLDWKP
                     GKGEKFSLFMSPATGKFTFVMDDTIASFEADTVNHKNRYGVTTGDNLRAEFGASLIAA
                     LNTSLGKNTSLSSKLTLFNNYTDPIADHRANIDVDLQNNLNIKVSKYIAATIFVQVLY
                     DHDIAIPTYDENDVQIGTGPKTQFKEVFGIGLSYKFE"
     gene            complement(1328..2617)
                     /locus_tag="PLM90_13010"
     CDS             complement(1328..2617)
                     /locus_tag="PLM90_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ90349.1"
                     /translation="MASGLHFIPVEEALQCIKSGDRVFVHGSACTPTYLLKHLAERHN
                     ELRDVELVFISVYGDMHVDKPEYRDSFKLNSMFVSNSIRADVNEGYADYIPIFLSEIP
                     ELFKRDILTLDVALVQVSPPDRHGYCSLGVSVDIARTAVNKAKYVIAQVNPQVPRTHG
                     ESLIHYSRFHCMVYADEPIHEAVFGEKVGPAELKIGEYIAGMIEDRSTLQMGIGAIPD
                     AVLKCLTNHKDLGVHTEMFSDGLVPLFHSDVVNNKYKKVHPNTTVSGFALGTRMLYDY
                     VDDNPAFEFLDIDYVNEPSVIKQNPKVVAINSCIEVDITGQVCSDSIGTYQYSGVGGQ
                     MDFIRGAALSEGGKPIIALSSRTAKGVSRLVPILKPGAGVVTTRAHVHYIVTEYGIAY
                     LFGKNLRQRAKALIEIAHPDDREMLHKAAFERFKIFV"
     gene            complement(2717..3691)
                     /locus_tag="PLM90_13015"
     CDS             complement(2717..3691)
                     /locus_tag="PLM90_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HMZ90350.1"
                     /translation="MNKGPISQFITHHYRHFNAAALVDAAKGYETHLLEGGKMMVTLA
                     GAMSTAELGISLAEMIRQGKIDIISCTGANLEEDIMNLVAHSHYKRVPNYRDLTPEQE
                     WELLEQGLNRVTDTCIPEEEAFRKIQHHIAAIWKDADAKGERYFPHEYMYKLLLSGVM
                     QADYEIDPKNSWMIAAAEKNIPIIVPGWEDSTMGNIFASYCIKGEMKTSTMKSGIEYM
                     IYLSEWYRANSSGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIHTPKFIVESDATIVAPLIFAYILGW"
     gene            complement(3897..>5264)
                     /gene="gcvPB"
                     /locus_tag="PLM90_13020"
     CDS             complement(3897..>5264)
                     /gene="gcvPB"
                     /locus_tag="PLM90_13020"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HMZ90351.1"
                     /translation="MIPLGSCTMKLNAASELLPITWPEFSAIHPFVPAEQVEGYLHII
                     KELEQDLCEITRFDGCSLQPNSGAQGEYAGLMVIRAYHIHHNQSHRNKVLIPASAHGT
                     NPASAVMAGMDVIVVKSDAEGHIDMDDLNAQAELHKDDLACLMVTYPSTHGVFEETIM
                     QICNIVHQHGGLVYMDGANMNAQVGLTSPAKIGADVCHLNLHKTFAIPHGGGGPGMGP
                     ICVNEKLVPFLPGHPLFKTGGINAIKAINAAPFGSASILLISYAYIKMLGGEGCTNAT
                     KYAILNANYLKSKLDKAYDVLYLGKSGFVAHEFIIDFRKWKHTIGLEVEDVAKRLMDY
                     GFHAPTVSFPVAGTLMIEPTESESKEELDRFVEAMLGIRSEIAALENGTADKLDNVLK
                     NAPHTIREVLSEHWTHGYSRDAAAFPASYIRERKFWPSVARINNTHGDRNLICTCPPT
                     EAYAS"
BASE COUNT         1542 a         1192 c         1017 g         1513 t
ORIGIN      
        1 atttcctcat cgtagcattt gtaatgttta tggtggtgaa agcaatgaat tccatgaaga
       61 aaaaagaaga ggaagcaccg gcagctccac ctgcaccacc tgcagacgtg gtattgttga
      121 ccgagattcg cgacctgctc aaaaaataaa catacacaca aaaaaatgcc ttccacccgc
      181 gagggcattt tttttggctt gattattgac catagcggga tgcttataat aaacttacta
      241 tcatgaaaaa aatgtgtacg cttatagcag ctatagccat caatgctgct gtttttgctc
      301 aaaccgactc tacctccaat tggaaaaaag gtggtggtgc tattctaaac ttcaaccaaa
      361 catcactcac caattgggca gcagggggtg aaagcaactt gtcaagtact atcctgacaa
      421 atttctatgc caattacaaa atgggtacta ctactttaga taatacccta aacatgaatt
      481 acggactcat cactgcaaac aactatgctg atattcgcaa gaatgaggac cgcatagaat
      541 taaatagcaa atacggtcgc tatgcgtttg ccgatgattt ctattattca gcattggtga
      601 atttccttac ccaatttgca cccggttatg actatttagc ggatcctgat gcattggtgc
      661 cgatttcaaa aatcatgtca ccgggatatc ttacggctgc cgcaggtctc gactggaagc
      721 ccggcaaagg tgagaaattt agtttgttca tgtcgcccgc aacaggaaaa tttaccttcg
      781 taatggatga tactatcgca tcatttgaag cagatacggt gaatcataaa aacagatatg
      841 gtgtgactac cggcgataat ctacgtgctg aatttggtgc ttcattgatc gcggcattaa
      901 atacctcact tggaaaaaat accagcctca gcagcaaact taccttattt aacaactaca
      961 ccgatccaat tgccgatcat cgtgcaaaca tcgatgttga cctccaaaat aatcttaata
     1021 ttaaagtgag taaatatatt gctgcaacca ttttcgtgca ggtgctgtat gatcatgata
     1081 ttgccattcc aacttacgat gaaaacgatg tccaaatcgg aaccggccct aaaacacaat
     1141 ttaaagaagt gtttggcatc ggcttgtcat acaaatttga ataaccaaat taatagctta
     1201 atgaaaaaaa cctgctgatg attattagca ggttttttta tacggttttt actgtttatg
     1261 gtatgtgcgt ttttctcgcg aagacgctaa gttgcaaggg cgcgaagttt gccgcgtacc
     1321 caaacattta tacaaaaatc ttaaaccgct caaaagccgc tttatgcaac atctccctgt
     1381 catcagggtg agcgatctcg atgagggctt tggcgcgttg gcgaagattt tttccgaaga
     1441 gataggctat accatattca gttacgatat aatgcacatg cgctctggtt gtaacaacac
     1501 ctgctcccgg ttttaaaatt ggcactaaac gggaaacgcc ttttgcagtg cgcgatgaca
     1561 gggcaataat aggtttacca ccttcactca aagcagcacc gcgaatgaaa tccatttgtc
     1621 cccctactcc actatattga taggtgccaa ttgaatctga gcatacctgt ccggtgatgt
     1681 ccacttcgat gcaagaatta atggcaacaa ctttgggatt ttgtttgata acgcttggtt
     1741 cgttcacata gtcaatatct aaaaattcga aggccggatt gtcatcaaca taatcatata
     1801 acatccttgt gcccaatgcg aaaccgctga ctgttgtatt cggatgcact tttttatatt
     1861 tattattcac cacatctgaa tgaaataacg gcaccaaccc atcgctgaac atttcagtat
     1921 ggacgccaag gtctttgtgg ttagtaagac attttaatac tgcatcagga atagcaccaa
     1981 tacccatttg caaggtactg cgatcctcga tcattccggc aatatattca ccaatcttta
     2041 actcggcagg tccaactttt tctccaaaaa cagcttcgtg aataggttca tctgcatata
     2101 ccatacaatg gaaacgcgag taatgtatga ggctttcacc atgcgtacga ggaacctgtg
     2161 gattcacttg tgcaatcacg tatttagcct tgtttacagc agtgcgtgct atgtcgacac
     2221 taacgcccaa ggaacaatat ccatgacgat caggaggtga tacttgtacc aatgctacat
     2281 ctagtgttag aatatcacgc ttgaataatt ccgggatttc actcagaaaa ataggaatgt
     2341 aatcagcata accttcattc acatcggcac gaatactgtt gctcacaaac atggaattga
     2401 gtttgaagct atcgcggtat tcaggtttgt cgacatgcat atcgccataa acactgatga
     2461 aaacgagctc tacatcgcgc aactcattgt gtcgctctgc caggtgcttt aataaatacg
     2521 ttggagtgca tgcactgcca tgaacaaata cacgatcgcc ggatttaatg cactgtaagg
     2581 cctcctccac aggaatgaaa tgtaatccgc ttgccataat attatctttt aaaaatcaaa
     2641 aaaaatcacc ggcatgcata aagcaaaccg gtgatgcaaa attagatgga agttaattgg
     2701 atatgattga ttaaaatcac catcctaaaa tatatgcaaa aattaatggc gctactatgg
     2761 tggcatcgct ttcaactata aacttaggcg tatgtatatc aagtttcccc caagttatct
     2821 tttcatttgg cacggctcct gaataagaac cgtaggatgt cgtgctgtcg ctgatttgac
     2881 agaagtagct ccagaatgga acatcatgcc attcgagatc ctgatacatc atcggcacca
     2941 cacaaatcgg gaaatcaccg gcaataccac caccgatctg gaagaagcct acaccctttc
     3001 cggatgaatt ggcgcgatac cattcgctca aatatatcat gtattcgata ccggatttca
     3061 tagtggatgt tttcatttca cccttaatgc aataggaagc aaaaatattt cccatggtgc
     3121 tatcttccca tcccggaacg atgattggaa tatttttttc tgcagcagca atcatccatg
     3181 aatttttggg atcgatctca taatctgctt gcatcacacc gctcaacaat aatttgtaca
     3241 tatactcatg cggaaaataa cgctcgccct ttgcatcagc atctttccat attgcagcaa
     3301 tatgatgttg aattttgcgg aatgcttctt cttcaggaat acaagtatct gttacgcggt
     3361 ttaagccttg ttccagtaat tcccactcct gctcaggtgt caggtcgcga taattaggaa
     3421 cgcgtttgta gtgagaatgc gctaccaggt tcatgatgtc ctcttcaagg ttggcaccgg
     3481 tgcagctgat gatgtcaatt ttaccctggc gtatcatctc ggcaagagaa ataccgagtt
     3541 cagcggtact catggcaccc gccagtgtta ccatcatttt gccgccttca agtaaatggg
     3601 tttcgtaacc ttttgctgca tctactaaag cagcggcatt aaagtgacgg taatgatgcg
     3661 taataaattg tgatattgga cccttgttca ttgctttgtt ttatgcgttt caaatatatt
     3721 aataatcaac tcattttttc ttacaagttg tgtttgatgt agtgcgcccg ggtgtcccaa
     3781 agatttgcgc aaaaaatccc gtccaactat aacgcagtta cagcaaagtt tggtcagtcc
     3841 cactactcac tactcactac ccaataccca ctactcacta ctagctattc actacatcag
     3901 gaagcatacg cttctgtcgg cggacaagtg caaatcaaat tcctatcacc gtgtgtatta
     3961 ttgatacggg caacagaagg ccagaatttg cgctcgcgta tatacgatgc agggaaagca
     4021 gcagcatcac gggaataacc atgagtccaa tgttcactca acacttcgcg aatagtatga
     4081 ggtgcatttt ttaggacatt atccaatttg tctgcagttc cgttttccaa cgcggcaatc
     4141 tcagacctga tacccaacat cgcttctaca aaacgatcaa gttcttcctt gctttcgctt
     4201 tcagttggct caatcatcaa tgtgccggca acagggaatg aaaccgttgg tgcgtggaaa
     4261 ccataatcca tcaaacgctt tgcaacatct tctacttcca atccgatagt atgcttccat
     4321 ttgcggaaat caatgataaa ttcgtgtgct acaaatccac tttttccgag atataacaca
     4381 tcgtaagctt tatctaattt gctcttgaga taatttgcat tcagaatagc atatttggta
     4441 gcattcgtgc agccttctcc acccaacatt ttaatataag cgtagctgat cagcagaata
     4501 cttgcgcttc caaaaggtgc tgcattaatg gccttgatag cattaatgcc accggtttta
     4561 aataatggat gtccgggtaa gaaaggaacc aatttttcat ttacgcaaat tggacccatg
     4621 ccgggacctc cacctccgtg tggaatggca aatgttttat gcagattcaa gtgacataca
     4681 tcagcaccaa tttttgcagg tgatgtcaat cccacctgag cattcatatt cgcgccgtcc
     4741 atgtacacca atccaccatg ttgatgaacg atattacata tctgcatgat tgtttcttca
     4801 aatacaccat gcgtacttgg ataggtaacc atcaaacaag caagatcgtc tttatgtaat
     4861 tcagcctgtg cattcaaatc atccatatca atatgacctt cggcatcgct ttttactacg
     4921 atcacatcca tacccgccat cacagcactg gcaggattgg tgccatgtgc acttgcagga
     4981 atgagcactt tatttctatg cgattgattg tgatgaatgt gataagcacg tatcaccatc
     5041 aaccctgcat attcgccctg tgctccggaa tttggttgca gactgcatcc gtcgaaacgt
     5101 gtgatctcgc aaaggtcctg ttcaagttct tttatgatat gtaaatatcc ttccacctgt
     5161 tcagcgggaa caaaaggatg tatcgctgaa aattcaggcc atgttatagg caataattct
     5221 gatgctgcat ttaatttcat cgtacaactg cctaaaggga tcat
//
LOCUS       DAOQOU010000201         5239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9658_length_5239_cov_5.357639, whole genome shotgun sequence.
ACCESSION   DAOQOU010000201 DAOQOU010000000
VERSION     DAOQOU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9658_length_5239_cov_5.357639"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..902)
                     /locus_tag="PLM90_13025"
     CDS             complement(63..902)
                     /locus_tag="PLM90_13025"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HMZ90352.1"
                     /translation="MSKKKQSSPESPGFLRFLMSKVFWLNVFGALVVAILLMWGTLFF
                     IQQYSRHGESQTVPNLIGKTTSEALNELDNLDLEYAVMDSTFDPDERPLTILNQDPLP
                     DSKVKSGRTIYVTVNMKQAPKTEIPRFEVGTSFISVREILESHGLKVGEIIYKPFEYR
                     DVYLDMRVHGEKRSLKPGTMIAKGTKIDVVLGNGLGDTKVIIPDLTGLTYIEAANLIQ
                     LKELSLGTVIAAGAISDSADAMVYKQFPTPGIDNAVNLGSMIDIWITDDPALLTPSSN
                     EDQ"
     gene            complement(936..1916)
                     /locus_tag="PLM90_13030"
     CDS             complement(936..1916)
                     /locus_tag="PLM90_13030"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMZ90353.1"
                     /translation="MKKNVAVVCGGYTAEREISLGSGDVVIRNLDESPFNVFKVVIDP
                     DGWFVEPEHIPVDMADFSFLWTGNVVKFDLVFNAIHGSPGEDGKLQGYLDMMGVPYTN
                     CGVATSALTFNKFWTKQVLDNHQIPMAKGRLVNKQNAEAMEQEISSQFQLPLFVKPNN
                     NGSSFGVSKVKDMRELSKAISEALKYDREALVEEGISGREVTCGVYRHEGNIKVLPIC
                     EVVTGKHDFFDYTAKYTAGESDEIIPAPIPETEAIRVRNYSTAIYEALNCRGVVRIDY
                     IIANGVPHFLEVNTVPGLSEASIVPKMAKTAGMTLNEFFTGLLDGASCDH"
     gene            complement(2034..2381)
                     /locus_tag="PLM90_13035"
     CDS             complement(2034..2381)
                     /locus_tag="PLM90_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ90354.1"
                     /translation="MAIHITSDCINCGACEPECPNNAIYEGGVEWAFADGTTVKGSVT
                     MMDGSSMDADQKNAPIADDIYYIVPDKCTECQGFHEEPQCAAVCPVDCCLPDPDHSET
                     PDQLLAKKAKMHI"
     gene            2524..3522
                     /locus_tag="PLM90_13040"
     CDS             2524..3522
                     /locus_tag="PLM90_13040"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HMZ90355.1"
                     /translation="MDTENRIAALVSLGECIQTYPDDLSEVVTRMGHVNGWFTETECK
                     RMLSSICGEFLQRDLLHTWMSNYQATETQKTIGIVMAGNVPFVGMHDLLCIIMSGHKA
                     QVKMSSKDNLFFPWMKEALSKISPPVSEQITFTEKLENFDAVIATGSNNSARYFEYYF
                     GKYPHIIRKNRTSIAILTGNESDDALYNLGKDVFWYYGLGCRNVGKVFIPEGYDKQHF
                     FRLWEDFRYVVDNTKYKHNYDYNRALLLLNKTNYLTNDFYMLVDSDQLYSPLSVLYAE
                     TYLHDADLQLKLESIGENLQCVVSSAPGNTPFGKTQEPRLSDYADHVDTMQFLMQV"
     gene            complement(3564..4844)
                     /locus_tag="PLM90_13045"
     CDS             complement(3564..4844)
                     /locus_tag="PLM90_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ90356.1"
                     /translation="MDTRRSFLRKSAALASLTAFTDLVLDDTLKAEVARVQHLDPLTV
                     ANDEEFWMNIRQAYTVSSTIINLNNGGVSPAPKVVQDAVDRYNKLTAEGPSYYMWQIL
                     DKGRESLRRKIAELSGADSEEIAINRNTTEALDNVIFGLDFEKGDEILLTKQDYPNMI
                     NAWKQREKRDGIVLKWIELDLPTDDVLYLVKKYTEQITSKTKLVQVNHIINWTGHINP
                     VKEIADAVHAKGPEVMIDGAHTMGHFVFNVPELGGDYYGSSLHKWTSASVGSGMLWIK
                     KEKIHKVWPLMGAPDTQDVNNIRKFENLGTRSFPIEYAIANAIDFHNMIGAARKEARL
                     RYLKEHWVNQCLDLPKFHLNTSMDPRFSCAIANFRIDGMEPGDIASKLITDYNIHCVA
                     INWENIHGVRIAPHVYTMPSELDLLAKAIKKLAS"
     gene            complement(4890..>5239)
                     /locus_tag="PLM90_13050"
     CDS             complement(4890..>5239)
                     /locus_tag="PLM90_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ90357.1"
                     /translation="HITALRTHMAKRLRDEIPGIIFNCPDDGLYTVLNVNFPPSESNE
                     LLLFNLDIAGIAVSGGSACSSGSNVGSHVIARLNREQNSAAVRFSFSKFNTMEEIDYT
                     VEKVKALFPAHVA"
BASE COUNT         1445 a         1141 c         1098 g         1555 t
ORIGIN      
        1 caagagagtg cggcgatgag gaaatgatat tttcttagca aatgcatgat ttaataatag
       61 aattattggt cttcgtttga ggatggcgtg agtaatgcag gatcatcggt tatccagata
      121 tcgatcattg atccgaggtt aacggcatta tcgattcccg gcgttggaaa ttgtttgtac
      181 accatggcat cagcagagtc gctaatggca cctgctgcaa taactgttcc cagagaaagc
      241 tctttcagct ggatcaaatt agctgcttcg atataggtaa gtcctgtaag atcgggtatg
      301 ataacttttg tatcaccaag accatttcct aacacgacat caattttagt tccttttgca
      361 atcatagtac cgggcttcaa tgagcgtttt tcgccatgta cgcgcatatc gagataaaca
      421 tcgcggtatt caaagggttt atatatgatc tcgccaactt tcaaaccatg tgattctaaa
      481 atctcgcgca cgctgataaa gcttgttccc acctcaaaac gaggaatttc cgtttttggt
      541 gcctgcttca tgttaacggt aacataaatt gttcttccac tcttcacttt tgagtcggga
      601 agcgggtcct gattaaggat ggtaagcggg cgttcgtcag gatcgaaggt agaatccatc
      661 accgcatact ccaggtcgag gttatctagt tcattcaatg cttctgaggt agtttttcct
      721 ataaggttcg gaactgtctg gctttcgccg tggcgtgagt attgctgaat aaagaacaga
      781 gttccccaca taagcaaaat ggccaccacg agtgctccga acacattgag ccagaacact
      841 ttactcatta aaaagcgcag gaagcccggg ctttcggggg aagattgttt tttcttactc
      901 atgcgtagcg cgcaaagtta cgtaaaatgg aggcttcaat ggtcgcagga agcaccatct
      961 aacagcccgg taaagaattc gtttaaggtc atgccggcag ttttcgccat tttcggcact
     1021 atactagcct cgctgagacc cggaacagtg ttcacttcca gaaaatgtgg cactccgttt
     1081 gcaatgatat aatcaatgcg caccaccccg cggcagttca atgcctcata aatagcggtt
     1141 gagtagttgc gtaccctaat cgcctctgtt tcaggaattg gggccggaat gatctcatca
     1201 ctctctcctg ccgtgtattt tgccgtgtag tcaaagaaat catgcttgcc ggttactacc
     1261 tcacaaatag gcaacacttt aatatttcct tcatgtctat ataccccaca ggtcacttct
     1321 ctaccgctta taccctcttc aaccaaggct tcgcggtcgt atttcagtgc ttctgagatt
     1381 gccttcgaca gttcgcgcat atctttcacc ttcgaaacgc cgaagctgga gccgttgtta
     1441 ttaggtttta cgaatagcgg caattgaaac tgactactga tttcctgctc catggcctcg
     1501 gcattttgct tgtttaccaa cctgccttta gccatgggaa tctggtgatt atcgagcact
     1561 tgtttggtcc agaatttatt gaatgtaagt gctgatgtag ctacaccgca attggtatat
     1621 ggcacaccca tcatatccag atatccttgc aatttcccgt cctcaccggg agagccgtga
     1681 atagcattaa aaaccagatc aaatttcacc acattaccgg tccacaaaaa gctgaaatcc
     1741 gccatatcaa ccggtatatg ctccggttct acaaaccagc catccggatc gataacaact
     1801 ttaaatacat tgaatggcga ttcatcgagg ttacgtatca ctacatcgcc actccctaat
     1861 gaaatttccc gttcagcggt atatccgccg cagaccacgg caacattctt tttcatggtg
     1921 gcaaattaac gcaaaaggtg atgcctgttg tatctgactt gtaaacaatt gatagtggta
     1981 aaataaaaaa aggcgccacc cggaggaagc gcctttcatt aatatgaggt ttttcaaatg
     2041 tgcatttttg ctttcttcgc aagaagctga tcaggtgttt cagaatgatc aggatcagga
     2101 agacaacagt caaccggaca aactgctgca cattgtggtt cttcgtggaa cccttgacat
     2161 tcggtgcatt tgtcgggaac gatgtaataa atatcatctg caataggtgc gttcttttgg
     2221 tcggcatcca tggaagagcc atccatcatg gttactgatc ctttaacggt ggtgccatcg
     2281 gcaaaagccc actccacgcc accttcatag atcgcattat ttggacactc gggttcacaa
     2341 gccccgcagt tgatacagtc tgatgttata tgaattgcca taataagtct ttcgctattt
     2401 ttgagcgaac aaatataaat gcaggctcgt cacattctca aaaaaacagc ctttttagga
     2461 ctgaatttat actcattcta aataacgttg ttgttgtgac gggtgtttaa tactgaaatg
     2521 actatggata ctgaaaatcg cattgctgcg ctggtgagcc ttggtgaatg tatacagacc
     2581 tatccggacg atctgtctga ggtagttacg cgcatggggc acgtcaacgg atggttcacg
     2641 gaaacggaat gcaaacgtat gttatcgagc atttgcggtg aatttttgca gcgagattta
     2701 cttcacacct ggatgtcgaa ttatcaggct actgaaaccc aaaaaacaat aggcattgta
     2761 atggccggaa atgtgccttt tgtaggtatg catgatttgt tgtgcattat tatgagtgga
     2821 cataaggcgc aagtgaaaat gagctcaaaa gacaatcttt tttttccgtg gatgaaggag
     2881 gcactatcaa agatttcacc tcctgtttcc gaacagatca cttttaccga aaagctcgaa
     2941 aattttgatg cggtgattgc aaccggcagc aataattcgg cgcgttattt tgaatattat
     3001 ttcggcaagt atccgcatat catccgcaaa aacagaactt ctattgcaat tttaaccgga
     3061 aatgagtctg atgatgcatt atataattta ggtaaagatg ttttttggta ttatggactt
     3121 ggatgtagaa acgtaggaaa agtttttatt cccgaagggt atgataagca acacttcttc
     3181 aggttatggg aagattttcg atatgtagtt gacaatacca aatacaagca taattacgat
     3241 tataatcgcg cattgctctt attgaataaa accaattacc tcaccaacga tttttatatg
     3301 ttggtagaca gtgaccaact atattcacca ttatcggtgt tgtatgcaga aacatattta
     3361 catgatgctg atctacaact aaaattggaa tccattggag aaaaccttca gtgtgttgtt
     3421 tcctctgctc cgggaaatac ccctttcgga aaaactcaag agccgcgttt atccgattat
     3481 gccgatcatg tggataccat gcaattttta atgcaggttt gaagttgata gatacattgc
     3541 tatctaagaa aaaactgtat tgcctaactc gccagttttt taatagcttt cgctaacaga
     3601 tctagttctg aaggcatggt gtacacatga ggtgcaatgc gcacaccgtg aatattttcc
     3661 cagttaatgg caacgcaatg tatattgtaa tcggtaatta atttcgaggc aatatcaccg
     3721 ggttccatgc catcaattct gaaatttgcg atagcacagg aaaaccttgg gtccatgctt
     3781 gtattgagat gaaatttagg caagtcgagg cattgattca cccaatgttc cttcaggtaa
     3841 cgcaatcttg cttcctttct cgcagcgcca atcatattat gaaaatcaat ggcattggca
     3901 atggcatatt ctatcggaaa cgaacgtgta ccaagatttt caaatttgcg aatattgttg
     3961 acatcttgcg tatccggtgc acccatgagt ggccatactt tgtgaatctt ttctttttta
     4021 atccataaca tgccactacc taccgaagca ctggtccatt tgtgtaaact tgaaccataa
     4081 taatcaccac cgagctccgg aacattaaac acaaaatgtc ccatggtatg tgcaccatca
     4141 atcattacct caggtccttt cgcatgaacg gcatctgcaa tttctttaac cgggttgata
     4201 tggcctgtcc aattgatgat atgatttacc tgaaccaatt ttgtttttga agtaatttgc
     4261 tccgtgtatt tttttacgag gtacaacaca tcatctgttg gcaaatcaag ttctatccat
     4321 ttaagtacaa tgccatctcg tttctcacgc tgcttccagg cattgatcat attcgggtaa
     4381 tcttgtttag taagcaaaat ctcatcaccc ttttcaaaat ctagtccgaa aatcacgtta
     4441 tcaagcgctt cagtggtatt tctgttgatg gcaatttcct cggagtcggc gccggacaat
     4501 tctgcaattt ttctgcggag gctttcacgt cctttatcca gtatttgcca catataataa
     4561 ctcggacctt cggcagtgag tttattatat cgatcaacag catcttgcac aacttttggt
     4621 gccggactta ctccgccgtt attcagatta ataatagtag atgaaacggt atatgcctga
     4681 cgaatattca tccagaactc ctcgtcgttg gcaacagtta gcggatcgag gtgttgtacg
     4741 cgtgcgactt ctgctttgag tgtatcatcc aaaacaaggt cggtaaaagc tgtcagcgat
     4801 gcgagcgctg ctgatttacg gagaaatgat ctgcgggtat ccataggagg gtattctcct
     4861 gcaagataac acatttcaaa ttgtgggaat caggctacgt gggcaggaaa tagtgctttc
     4921 actttttcaa cggtgtagtc aatttcttcc atggtattga atttgctgaa agaaaagcgc
     4981 actgccgcac tattttgctc tctattaagt cttgcaatca catgcgagcc gacattactt
     5041 ccgctgctgc atgcgcttcc gccacttact gctattccgg caatatcaag attaaataaa
     5101 agaagctcgt tgctttctga aggaggaaaa ttcacgttaa gaacggtata caaaccatca
     5161 tccggacaat taaaaatgat accgggaatt tcatcgcgca gccgcttcgc catatgtgtg
     5221 cgcagagctg tgatatgtg
//
LOCUS       DAOQOU010000202         5172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9864_length_5172_cov_4.773109, whole genome shotgun sequence.
ACCESSION   DAOQOU010000202 DAOQOU010000000
VERSION     DAOQOU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9864_length_5172_cov_4.773109"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1350
                     /gene="rlmN"
                     /locus_tag="PLM90_13055"
     CDS             316..1350
                     /gene="rlmN"
                     /locus_tag="PLM90_13055"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMZ90358.1"
                     /translation="MAALPCIRSFNQTELAAFIAEMGEPAYRARQIYAWLWKKGALSI
                     DEMTDLPLALREKLKAAVTMEALSVEIEQKSSDGTIKVRFLTHDGHRIEGVMIPADDR
                     MTACVSSQVGCNLACTFCATGFIKVKRNLEPWEIVDQVVLLNRAAMKYHGRGLTNIVY
                     MGMGEPLLNYKNVLASVERITAEDGLFMSPKRITVSTAGIAKMIRKLADDGVRFNLAL
                     SLHAADDIKRSKLMPINDANNLNDLVMALNYFYIKTGNRITFEYILFDGINESMDDAK
                     NLVALCAKVPARVNIIEYNAIAEADFSKSKEAQREAFINYLEKHDIIAVVRRSRGKDI
                     DAACGQLANK"
     gene            1356..1595
                     /locus_tag="PLM90_13060"
     CDS             1356..1595
                     /locus_tag="PLM90_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90359.1"
                     /translation="MRSGRNSIIGITILMLTLVSTALLSCEKEYQCDCYQDVMGIDTS
                     YSLVGHGRDAQDVCNAFDSETTEFGITKTVDCEPY"
     gene            1598..2500
                     /locus_tag="PLM90_13065"
     CDS             1598..2500
                     /locus_tag="PLM90_13065"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HMZ90360.1"
                     /translation="MPAVAKHIARILIGITFITSAVLKMIGVDAFEVYVFSTGWFGLP
                     FATILARLIISVEGMLGLLLLLQYRYDVVKKITTGTLAVFSIYLIYLISAGRTENCQC
                     FGTMISLTPIESLVKNLALFVLLFISWKCPVIKIPQQASIALFVIVFTLVLPSFISPP
                     DFIVKYPDMPESTLQIAEKRIANNPVMQANQVTEGKKLVCMFSTHCSYCKHAAEKVSI
                     IAERLNLDDEVVYVFTGDEQELPQFWQESRSNQFHYFFLPMRNFFEIAGPSVPSIYLV
                     DGGKVTTHFHYRNIDETAIKSFFE"
     gene            2503..4347
                     /locus_tag="PLM90_13070"
     CDS             2503..4347
                     /locus_tag="PLM90_13070"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMZ90361.1"
                     /translation="MKRTFFFLTIFFITYSISAQSLRCTADSLLAPFYHGVASGDPTD
                     HSVLLWTRITTDSVSPEISWRVATDTLFTDIIANGTTITNIDSDYCVHVNVSGLLPYT
                     TYYYQFTYGGFHSLTGRTRTLPVGDIDSLRFAVISCASLNAGYFNVYDIITHRNDFDA
                     VIFLGDYIYEYETTGYAYNPAVDRPYDPEEEIIDLSDYRTRHSQYKLDDELRVLHQNF
                     PFICLWDDHEFADNAWNYGAENHQPVSEGDWLTRVAAAEEAYFDWMPVQKNNDTIFPF
                     TTYRSFALGDLMDLWVLDTRIDDRDKQVSYFDFDWDDSSRHIISLTQRNWLFDGLKNS
                     DAQWKVIGQQVMVSPLTALGVPINDDQWDGYPKDQEALLQFLRDSIPNNKVILTGDIH
                     SSWANDVATPSYDAGTHEGSAAVEFVGPSVTSPSMDLGWAEDVIMSMNEHIKFIDIEH
                     RGFLMLDVTKEKVQGDYYFINNIETPDTLYNYDDSWMILNGNNWLYHADTATVRAMDR
                     MVPLAPACPAAPDTATVNIIAPDVVILGVYPNPVHEELTVQFSLQQRSTMLLTIRDMQ
                     KQVIRHEVITADAGLHVIKLSVDKLSSGTYFLELTQGEQSLVRTFVKR"
     gene            complement(4443..5027)
                     /locus_tag="PLM90_13075"
     CDS             complement(4443..5027)
                     /locus_tag="PLM90_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMZ90362.1"
                     /translation="MSNFIISIYTEATPNPDSLKFVMNKMLLTGRSVDFERGDDVSYA
                     PLAEAIFNEFDFAKGVFIMNNFVTVRKQGEVDWYEVKSKVSEFIKNFVSEGNTVVGEI
                     PEIDNISGEMDGDAVVAKIRQMLDQYVKPAVEMDGGAIQFKSFDNGIVTLIMQGSCSG
                     CPSSTVTLKSGIEGLLKRMVPEVKEVVAESMEIM"
BASE COUNT         1409 a         1070 c         1200 g         1493 t
ORIGIN      
        1 attctagatg aaaaagacct gtttaaggtc taaaggcctc tgcgcctctg cgtgaaactc
       61 ggcattccaa cctgatcata cagctgaagt tgtatataat gaacaagact taaacaattt
      121 cccaccggcc atgtattatt tgcaattatt gcaactggaa aaggtggggt atgggtgttt
      181 tgtgaaggaa taaaacgcgg ctattgatac tgagtagtta gtcgtgtttg tttcacgcag
      241 agtcgcagag ccgcagagga acgccctctc tcctctctcc tttctcctct ctcctttaaa
      301 ccctaccttt gtagaatggc tgctcttcct tgtattcgct catttaacca gactgaactg
      361 gctgctttca ttgctgagat gggagagcct gcctataggg ctcggcagat ctatgcctgg
      421 ttgtggaaga agggcgccct gagtatcgac gaaatgaccg acctgcccct ggccctgcgc
      481 gagaaactga aggctgccgt taccatggaa gccctaagcg ttgaaataga acaaaagagc
      541 agcgacggaa ccatcaaagt gcgctttttg acccacgacg ggcatcgcat tgagggggtg
      601 atgatacctg cagatgacag gatgacagcc tgtgtgtcga gtcaggtggg atgtaacctg
      661 gcatgtactt tttgtgccac gggttttatc aaagtgaaac gcaacctcga accttgggag
      721 atcgtagatc aggtggtttt gctcaatcgc gcagcaatga aataccatgg tcgcgggttg
      781 acgaatatcg tttacatggg tatgggtgag cccctgctga attacaaaaa tgtgctagca
      841 tcggtagagc gcatcacggc agaggatggt ttattcatgt cgccgaagcg catcacagtc
      901 tctacggcag gaattgccaa gatgatcaga aaacttgctg acgatggtgt gcgattcaat
      961 ctcgcattgt cgctgcatgc tgctgatgat atcaaacgca gtaaactgat gcccatcaac
     1021 gatgctaaca atctgaatga tctggtgatg gcgctgaatt atttttatat caaaacaggt
     1081 aatcgcatca cctttgaata tattttattc gatggcatca acgagagcat ggacgatgcg
     1141 aaaaatcttg ttgcactatg cgccaaggtg ccggcaaggg tgaacatcat tgaatacaat
     1201 gccatcgcgg aagcagattt cagcaaatcg aaagaagcac aacgcgaagc atttatcaat
     1261 tacctcgaaa aacacgatat catcgctgta gtgcgccgca gcagagggaa agatattgat
     1321 gccgcttgcg gacaactcgc aaataaataa acttcatgag atccggcaga aacagcatta
     1381 tcggcataac aattttgatg ttgaccttgg tttcaacagc attattgtcg tgcgaaaaag
     1441 aatatcagtg cgactgctat caggatgtga tgggtatcga tacttcgtat tcactggtag
     1501 gtcacggtcg cgatgcacag gatgtgtgta atgcattcga ctccgagaca actgaattcg
     1561 gcatcaccaa aaccgtagac tgcgaaccat attgatcatg cctgctgttg ccaaacatat
     1621 tgcccgcata ctcattggca tcacattcat tacatccgcc gtactgaaaa tgatcggggt
     1681 ggatgctttt gaagtgtatg tattttctac cggttggttc ggactgccat ttgcaaccat
     1741 acttgcacgt ctcatcattt ctgttgaagg catgttgggt ttgttgcttc tattgcaata
     1801 caggtatgat gttgttaaaa aaatcaccac aggcacactc gctgtattca gcatttatct
     1861 catttattta atttctgcag gtcgcacgga aaattgtcag tgttttggta cgatgatcag
     1921 ccttacacca atcgaatcat tggtgaaaaa tctggctcta tttgttttat tatttatttc
     1981 ttggaaatgt cctgtgatta aaattccaca acaagcaagt attgcattat ttgttatcgt
     2041 attcacattg gtattacctt catttatatc acctccggat tttattgtta agtatcccga
     2101 tatgcccgag agcacgctcc aaattgcaga gaaacggata gctaataatc ctgtgatgca
     2161 ggcaaatcag gttactgaag ggaaaaaact tgtttgcatg ttcagcacgc attgttcata
     2221 ttgtaaacat gcagcggaga aagtttccat cattgctgaa agattaaatt tagacgatga
     2281 agtagtctat gttttcacag gagatgagca ggagctacca caattctggc aagaaagcag
     2341 atcaaatcaa tttcattatt tcttcctccc catgcgcaac ttttttgaaa ttgcgggacc
     2401 ttctgttccg agtatttatt tagtggatgg cggtaaagtg actacgcatt tccactatcg
     2461 caacatagat gaaacggcaa taaaatcttt ttttgaatga ccatgaaacg cactttcttt
     2521 ttccttacca tttttttcat cacctatagc atcagtgcac aatcgctgcg atgcacggca
     2581 gatagtttac tggcaccttt ttaccatggt gttgcatccg gcgatcctac cgaccacagt
     2641 gtgttgttgt ggacgcgcat caccacagat tctgtttcgc cggaaatatc ctggcgtgtg
     2701 gctaccgata ctttatttac agatataatc gcaaacggca caaccatcac taatattgat
     2761 tcggattatt gcgtgcatgt aaatgttagc ggattattac cttacacaac gtattactat
     2821 caatttactt atggtggatt tcattcgctc acaggaagaa cacgcacgct gccggttggt
     2881 gatatcgaca gtctgcgatt tgcggtgatc agttgtgcga gtctgaatgc cggatatttc
     2941 aacgtgtatg acatcatcac acatcgcaat gattttgatg cagtgatatt tcttggtgat
     3001 tatatctatg agtatgagac aacaggatat gcctacaatc ctgctgtcga caggccctac
     3061 gatcccgaag aagagatcat agacctgagt gattaccgca cacgacattc gcaatataag
     3121 ctggatgatg agctgcgtgt gctgcatcag aattttccgt tcatctgctt gtgggatgat
     3181 catgaatttg ccgacaatgc atggaattac ggtgcagaaa atcatcagcc tgtttcggag
     3241 ggcgattggt taactcgtgt agctgcagcc gaagaagcat atttcgattg gatgccggtg
     3301 cagaaaaata atgatactat ttttccattt accacctatc gctcatttgc attgggtgat
     3361 ctgatggatc tctgggtgct cgacacacgc atcgacgatc gcgataagca ggtgagttat
     3421 tttgatttcg attgggatga ttcatcgcga catatcatca gtttgacaca gcgcaattgg
     3481 ttattcgatg gattgaagaa ttcggatgcg cagtggaagg tgatcggtca gcaggtaatg
     3541 gtatcgccgc tgacagcatt gggggtgcct atcaatgatg atcaatggga tggctatccg
     3601 aaggatcagg aggcattgtt gcaatttttg cgtgattcca ttccgaataa taaggtgatt
     3661 ttaacaggtg atattcactc ttcctgggcg aatgatgtgg ctacaccaag ttatgatgcg
     3721 ggcacgcatg agggaagtgc tgctgttgaa tttgtgggac ccagtgttac atcgccaagc
     3781 atggacctgg gatgggcaga ggatgtgatc atgtcgatga atgagcatat taaattcatc
     3841 gacattgaac atcgcggatt tttgatgttg gatgtaacga aggaaaaggt gcagggtgat
     3901 tattatttca tcaataatat tgaaacacct gatacgcttt ataattatga tgatagttgg
     3961 atgatattga atgggaataa ctggttatat catgcagata ccgcaacggt gcgtgcgatg
     4021 gatcgtatgg ttccattggc acctgcttgt cctgcagcac ctgatacggc tacagtaaat
     4081 attatcgcac cggatgtggt gatattgggt gtgtatccaa atcctgtgca tgaggagttg
     4141 acggtgcaat tttcgttgca acaacgatca accatgttgc tcacgattcg tgatatgcaa
     4201 aagcaggtga taaggcatga agtaattact gcagatgcgg gattacatgt gataaaatta
     4261 tcagtagata aattatcatc aggcacctac ttcctggaac taacacaagg ggagcaatcg
     4321 ctggtaagga cttttgttaa gcgataggga ttattctatt tgtttttcac gcagaggcgc
     4381 agagtcgcag aggtttgaat acatgtattc ccacattctc acattcccac attcccacat
     4441 tctcacatta tctccatgct ttccgctacc acttctttca cttcaggaac catacgcttg
     4501 agcaagcctt cgatgcctga ttttagtgta acagttgatg atgggcatcc actgcaagat
     4561 ccttgcatga ttaatgtaac gatgccatta tcaaatgatt taaattggat agcgccgccg
     4621 tccatttcaa ctgcaggttt tacatattga tcgagcattt ggcggatctt ggcaacaaca
     4681 gcatcgccgt ccatttcgcc gctgatattg tcgatttcag gaatttcacc aacaacggta
     4741 tttccttcag aaacaaaatt tttgataaac tcgctcactt ttgatttcac ttcataccaa
     4801 tcaacctcgc cctgtttgcg tacggtaacg aagttgttca tgatgaatac gcccttagca
     4861 aagtcgaact cgttgaaaat agcttctgcc aaaggggcgt agcttacgtc atcaccgcgc
     4921 tcgaagtcga cactgcggcc ggtgaggagc attttgttca tgacgaattt gagggaatcc
     4981 gggtttggtg ttgcctcggt atatatgctg atgatgaaat tgctcatgtt atagtctttg
     5041 agtatgcaaa ggtaagaacc atttggatag ggaataagtt tgttaaactg aaaaaaaatg
     5101 cggggttcac gcagagccgc agagaccttt agaccttaaa caggtctttt tcatctagaa
     5161 tgttagtttt aa
//
LOCUS       DAOQOU010000203         5157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_9915_length_5157_cov_5.406899, whole genome shotgun sequence.
ACCESSION   DAOQOU010000203 DAOQOU010000000
VERSION     DAOQOU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9915_length_5157_cov_5.406899"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PLM90_13080"
     CDS             complement(<1..1113)
                     /locus_tag="PLM90_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90363.1"
                     /translation="MKYFAHALKIVLLLLLVCCFNIPEAEAHVKWFTDGSYADRPLRL
                     GEILEGRFFYTLFFVTLGTVAIGVWLDARISKANWYMRVDNWLDSRKSYATIVLRVAA
                     AMVLILSWQSNAMIVPTIPIPANYEWLGWFQFLMAFMLLMPKTVPIGGLGIVVIWVIG
                     NITLHPFHMLDYLMFAGVGVYLVMASIKNGKYKDSGLVVLYVTVGFSLCWVALEKFVY
                     PNWSLFLIKEHPNLAMGLNFDFFLKSVGFVEFGLGFLLLVCLLQRPLAIIITVTFFIT
                     SSVFGKVEIIGHTLIHGALIVFLLEGPGRFYLQLRQRFSKMTMRIGFTIVNFTLLFVV
                     LTLGYTTLANNKYEKKIEFLSKKPEFQRGQIELAGYP"
     gene            1417..3930
                     /locus_tag="PLM90_13085"
     CDS             1417..3930
                     /locus_tag="PLM90_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ90364.1"
                     /translation="MKSLILFTLLLFSISRLHAQQPNIIFIITDDMNDYVGVLGGHPQ
                     VHTPNIDSLAKRGVTFINAFSSAPGCAPSRTSMLSGKDCGYTKVYSNEGYQSHFRDNF
                     SDVLGNSVVYTIPEILKDSGEYFTYGLGKIFHSPTENDYDKTGPTCEKEQSWNRMYAI
                     KSAPGYDELTESYKYGGIFDFGKTPDSFEEMMDDRIAVDSAVAFINSYAAHTVNTCDR
                     PFFLALGIHKPHTERFVPEHYFPQYYADDLLSDSFAIIYNNPPGATPFNGLVMPPQPD
                     PMFDDFYQLPEGGIAQNFAANNDTYALLNDYVDDLPYLPEIGEGLTTEEKVAILEETM
                     RANYVSAYIAAVQFADSMVGLVMEALYAHPELEENTIVVFASDHGYSLGEKRHWTKWT
                     LWETDLRMPLIISHPDYPQNVVSKAVVSLLDLFPTFCDMTGVPYPTMPDGSPYLDGHS
                     LVPLLTQPSMNFSNAALSSYRKSSGFGNCFPQYSVRTERFHYIRYQTNNANISGAGAC
                     DYTTPHYVEELYDIGSDRQTDPNEWHNIADDPNYAPVIDYMEEFIPGGTMYNQRPFTV
                     NIYQAPMPCLLEFDDVLQMRGLLLNDNGELIDSTLGNYTFTWTNNLTEEIHIGYDYNF
                     SLSSIPSDVFDDAKEIFIYLTVTETITGRTIAFKTKQNQLHGDAIPHLSYVVNIADHT
                     IAVDDITLTGNYQTINWDFGDSYKSSGLIPGPHTFNTAGYHTIDVTIEYGNKCYLSDT
                     RTFYIDSVIFKSTELFSDIKIFPNPASDVVIVSSDDINGKIQLRVFNAMGELVMSVSY
                     KDALESIQLQVDHLPAGVYLLQMERNGHVKEEMIEVCRN"
     gene            4038..>5157
                     /locus_tag="PLM90_13090"
     CDS             4038..>5157
                     /locus_tag="PLM90_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90365.1"
                     /translation="MQKFYSSIAIAFICLSLQSLKAQGVCNPAGNVIIYSNYDGGNMT
                     INIDENIPDLHIGICSYESINVTFTGAYVDNITEVLYAGYDNDGTTSITGVDAGIADI
                     LLYPPVTLFDADGYPYMICAYECDSTYVPGGCNTVDQAVDYFMTTFGGSMRYSYFQYG
                     VFTGTYMMSAGGNCCFGASCTTSIDAGQDETICLGDSTQLDVLGGLTFNWSPATGLSN
                     AAIQDPFASPTETTTYVVTATDADGCVGIDSVTVFVLPLPVADITVTGNVLTATGGVS
                     YQWYQDGALIPGATGSSFVVTLDGTYTVLVTDASGCSAMSSAITMDINAIDDLSYHAS
                     ITIYPNPANAIVQISANIDISTTNYFVSDVTGKTIISDK"
BASE COUNT         1568 a         1042 c         1064 g         1483 t
ORIGIN      
        1 tggataaccg gcaagttcta tctgtccacg ctgaaattca ggctttttgc ttagaaattc
       61 aatcttcttt tcatacttat tatttgcaag agtggtataa cccaatgtga gcacaacaaa
      121 cagcaaggta aagtttacga tggtaaaacc aatgcgcatg gtcattttgc taaaacgctg
      181 acgcaattgc aaataaaagc gccccggacc ttcgagcagg aacacgatca aagcgccgtg
      241 aatgagtgta tgcccaataa tttcgacctt cccgaataca gaggatgtaa taaagaaggt
      301 tacagttatg ataatggcca atggacgttg gagtaagcaa accaacaaca aaaaccctag
      361 cccgaattct acgaatccaa cggatttgag gaagaagtcg aaattcaacc ccattgccag
      421 attcggatgt tctttgataa ggaaaaggct ccaattagga tacacgaatt tttcgagtgc
      481 cacccaacag agagagaagc caaccgttac atacaacact accagtcctg agtctttata
      541 cttcccgttt ttaatggatg ccatcaccag gtacacacca acacctgcaa acatgaggta
      601 atcgagcatg tggaagggat gcagcgtgat attaccgatc acccagatca ccacgatacc
      661 aagccctcca atcggcacag tctttggcat cagcaacatg aaagccatga gaaactgaaa
      721 ccagccgagc cactcgtaat ttgcgggaat tggaatagtt ggcactatca tagcattact
      781 ttgccatgaa aggatcaaca ccatagctgc agcaacacgc agtacaatag tggcgtagga
      841 tttgcgcgag tcgagccaat tgtcgacacg catataccaa ttagccttgg aaatacgtgc
      901 atcaagccat acaccgattg caacagtccc cagggtgaca aaaaacaagg tatagaagaa
      961 ccggccttca agaatttcgc cgagtctgag tggcctatcg gcgtaactac catcggtaaa
     1021 ccacttcacg tgcgcttcag cttcaggaat gttgaaacaa cataccagca atagcagcag
     1081 cacgatcttt aatgcgtgtg caaagtactt cataacaaca ggacaagggt attggcaaag
     1141 ctaaacactg ctattgcgcc taatgccaag gcagtgtaaa agaaatgtaa acagccatat
     1201 atgttcttca caattttcgg cgtaatgacg cttggtgagg acaatctatg gtttttacct
     1261 gcatgggaat tccaagtcaa ttccccccaa ttaattgggt aacaaaacca cttcacacag
     1321 ttataaggtt gtttcacatg aaaatgacgt ggtcttgata agaatcggtt gcacgaaccg
     1381 gcagttggct gcagttttga tttacaattc ccgatgatga aatcactgat actttttacc
     1441 ctgctattat ttagcatttc aagattgcat gcacaacaac ccaatatcat ttttatcata
     1501 accgacgata tgaatgatta tgttggggtt cttggtggac atcctcaggt tcacacgcct
     1561 aatattgaca gtcttgctaa acgcggtgta acctttatta atgcattcag cagtgctccc
     1621 ggttgtgcac catcgcgaac aagtatgtta agcggaaaag attgcggata tactaaggta
     1681 tattcaaatg aaggatatca aagtcatttc agagataatt tctccgatgt gctgggcaat
     1741 tcggtggtat ataccattcc ggaaattcta aaagacagtg gtgaatattt cacttatggt
     1801 ttgggaaaaa tatttcattc accaactgaa aacgattacg acaaaacagg tccaacatgc
     1861 gaaaaggaac aatcgtggaa ccgcatgtat gccataaagt cggcgccggg ttatgatgaa
     1921 ctaactgaaa gttacaagta tggcggtatt ttcgattttg ggaaaacacc tgattcattc
     1981 gaagaaatga tggatgacag aattgctgtt gattcagcag tggcatttat caatagttat
     2041 gcggctcata ctgtaaatac ttgcgacaga ccattttttc ttgcgttagg cattcataag
     2101 ccgcatactg aaagatttgt acctgagcat tatttcccgc aatattatgc tgatgattta
     2161 ttgagtgatt catttgctat tatttataat aatcctccgg gagctactcc ttttaatggc
     2221 ttggttatgc cgccgcaacc ggatcccatg tttgacgatt tctatcaatt gcctgaagga
     2281 ggcatcgcac aaaactttgc agctaataat gatacttatg cattgctaaa tgattatgta
     2341 gatgaccttc cctatttacc tgaaattggc gaaggattaa ccaccgaaga aaaagttgct
     2401 attttagaag aaaccatgcg tgcaaattat gtatctgctt atatagcagc agtacaattt
     2461 gcagattcca tggttggttt ggtgatggag gccttatacg cgcatcccga attggaagaa
     2521 aacaccattg ttgtttttgc aagtgatcat ggatattcat tgggtgaaaa acgtcactgg
     2581 acaaaatgga ctttatggga aacggattta cgtatgccat taattatttc gcatcccgat
     2641 tacccgcaaa atgttgtatc aaaagccgtg gtttcgttat tagatttgtt tcctactttc
     2701 tgcgatatga caggtgtacc ctatcctacc atgcctgatg gctcaccgta tcttgatgga
     2761 catagcctag tgccgttgct tacacaacct tccatgaatt tttctaatgc cgcattgagt
     2821 tcttatcgca aaagttctgg ttttggaaat tgtttcccgc aatattccgt tcgaacagag
     2881 cgatttcatt atatcagata tcaaactaat aacgcaaaca tcagtggtgc cggagcttgt
     2941 gattacacta cacctcatta tgtagaagaa ttatatgata tcggcagcga ccgtcaaacg
     3001 gatcctaatg aatggcataa tatcgccgac gatccaaatt acgcgccggt tattgattat
     3061 atggaagagt tcatacccgg cggtaccatg tacaaccaac gaccatttac ggtgaatatt
     3121 tatcaggctc caatgccttg cttgcttgaa tttgatgatg ttctacaaat gcggggccta
     3181 ttactcaatg ataacggaga attaattgat tctacattgg gaaattacac atttacgtgg
     3241 acaaataatc ttactgaaga aattcatatc ggctacgatt ataatttcag tctgtcaagt
     3301 attccttccg atgtatttga tgatgcaaag gagatattta tttatcttac ggttacagaa
     3361 actataaccg gtagaaccat tgcatttaaa acaaaacaaa atcaattaca tggcgatgcc
     3421 attccgcatt taagttatgt ggttaatata gcagatcata caattgcggt tgatgatatc
     3481 acattgacag gaaactatca aaccattaat tgggatttcg gcgacagcta taaatcatca
     3541 ggtttgattc ccggacccca tactttcaat acagccggct accatacaat tgatgtcact
     3601 attgaatatg gcaacaaatg ctatctctct gataccagga cattttatat tgacagtgtg
     3661 atatttaaat cgacagagtt gttttccgat atcaaaatat tcccaaatcc ggcatctgat
     3721 gtggtaatcg tgagctcaga tgatattaat ggcaaaatcc aacttcgagt attcaatgct
     3781 atgggcgaac ttgtaatgtc tgtatcttat aaagatgcat tagaatccat tcaattacag
     3841 gtagatcatt tacctgcagg ggtttatctt ttacaaatgg aacgcaatgg ccatgtgaag
     3901 gaagaaatga tagaggtatg taggaattag atacattaat aaattcccgg gagagttgtc
     3961 ttaccgggat gtaatgttaa aaggaaatga taattaggat ggttatggcg tttttgttat
     4021 ttttataaaa aaaacgcatg caaaagttct actcctcaat cgcaatcgca ttcatttgcc
     4081 tgtcacttca atcgcttaaa gctcagggcg tttgcaatcc cgccggtaat gtgatcattt
     4141 attctaacta tgatggtggg aatatgacca tcaatattga tgagaatatt ccggatctgc
     4201 acattggcat ttgttcatat gaatctatca atgtaacatt tacaggtgcc tatgtcgata
     4261 acattaccga agtgctgtat gccggttacg acaatgatgg aacaacgagc attacgggtg
     4321 ttgatgcagg aattgccgac attttattgt atccaccggt aactttattt gatgccgatg
     4381 gttatccata catgatttgc gcttatgaat gcgactcaac ctatgttccc ggaggttgta
     4441 atactgttga tcaggctgta gactatttta tgaccacctt tggcggcagc atgcggtatt
     4501 catattttca atatggtgta tttacaggaa cgtatatgat gagtgccgga ggaaattgtt
     4561 gtttcggcgc ttcatgcacc acatcaatag atgccggtca ggatgaaact atttgtttgg
     4621 gcgattcaac tcaacttgat gtacttggcg gattgacatt taattggagc ccggcaacag
     4681 gattgagcaa cgctgccata caagatccat ttgcctcccc tacagaaacc acaacttacg
     4741 tggttacagc aactgatgca gatggttgtg tgggaattga ttcagttacc gtgttcgtac
     4801 ttccattgcc tgtagcggat attacagtaa caggcaatgt attaacagct accggcggcg
     4861 tgagttatca atggtatcag gatggagcat taattccggg tgcaacgggc agcagctttg
     4921 tagttacact agacggcacc tatacagttc ttgttacaga tgcaagtggc tgttctgcga
     4981 tgagttcagc aatcaccatg gatatcaatg ccatagatga cctgtcatat catgcttcga
     5041 taactattta tccaaatccc gcaaatgcta tagtacaaat cagtgcaaat attgatattt
     5101 ctactaccaa ctatttcgtt agtgatgtga ccggtaaaac aatcatcagc gataaat
//
LOCUS       DAOQOU010000204         5110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10068_length_5110_cov_7.759644, whole genome shotgun sequence.
ACCESSION   DAOQOU010000204 DAOQOU010000000
VERSION     DAOQOU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10068_length_5110_cov_7.759644"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PLM90_13095"
     CDS             <1..1207
                     /locus_tag="PLM90_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90366.1"
                     /translation="VIECLAGSNIYAQVQFLDQKNGMWNASLCLLKHYESKADTILNA
                     EEAFKFYRYYFEWMMSIANAIASDADIDKLAKKLLSKCPAVSYNETQLWIKIVLFRKD
                     INVTTTRADYTNPKIAEALQSRLHDLIYEADAINSVYCLYKAKLTGIFLCNLTQNFSQ
                     SRIYISDINELFAQSPGAFTESELLTAQWHYAQILFFSSQFDESFVIYEQLFQKINVS
                     ETMRWYVFIAEFFQNCLVTGHYDQAQQLCTDYFQKFFDDKNGSFYLSALIQCVKLLIH
                     TSDFEKAKLKLDELNKLTTKTSSLQFQFALRELTSAYYYLTGNYKEALILAEKNLKFM
                     RSKRIHYLIPEYTFHSRLIKALIKQKQRQKSFTEEEVKMMDAMQQGTMAQYGLLLKRL
                     EKNLNYFIV"
     gene            complement(1293..3473)
                     /locus_tag="PLM90_13100"
     CDS             complement(1293..3473)
                     /locus_tag="PLM90_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ90367.1"
                     /translation="MKQLLRKSIQLCLSLMLLSSPIIIQAQTNPTPQAVPYTQDFSGL
                     AHASTTYPAGIQGWKCSSAATTSFVTTGPTGDQALIASSSASNNSGGVHNYNGKIGLL
                     ASGSTNPGIVLSLNTTGYSNLLLSYDIMVIRNPYDGTTNTRLNEATVQYRIGNTGAWI
                     TLTGIEYQSGTTLQTGSGVTTPQNPLSRSILLPDATDNVAEVQVRWISRDMTGGGSRP
                     SFAIDNISVQPDADGDGYSVVTDCNDANALVYPGAPENCNGYDDDCDGLIDEGTMVTY
                     FADADGDLYGDPATTAVACVAPTGFVGNDEDCNDADAMINPAATEVCNGYDDDCDGLV
                     DDDDPSAEGLPLWFEDLDGDTFGNSDVFVFACNMPTGYSIDPGDCDDTNAGVNILATE
                     ICNGIDDNCDGNIDEDLIFATWYADSDGDGYGDAASTFFTCDPAPVGYVMDATDCNDA
                     AATVYPGATEICDGLDNDCDGDIDEDVVSATITPAGVVSTCKGVNTTLTANAGESYTY
                     QWFKNGNIIAGATDVTFSSNKPGYYQVQVNLPEGCFALSPVTTITVSPNPNANISAPN
                     GTSLCATVKLKASYDASYTYQWYDGAGEIAGATAYQYFPTMAGNYYCIVTNAFGCSRN
                     TAEITVTACREENATANNNLEVYPNPTSGQLHLNLSTTAKEGTASITIVNMLGEIVYS
                     TVADYSNFELDQTLDLTGVIPAGMYSVIVIQNNTIAESRIVIQN"
     gene            complement(3672..>5110)
                     /locus_tag="PLM90_13105"
     CDS             complement(3672..>5110)
                     /locus_tag="PLM90_13105"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ90368.1"
                     /translation="ISADGFQMPPLRQYPKRYAYSTNGGLSWTNNGSDYSIPSGSCGS
                     GTTFTWDLPDFTTASPVLIRIYGWNANNVNGQSRIYNGVVSGSTCALTTWYADADGDG
                     YGNAAVSTVACNAPVGYVADNTDCNDANASINPGMAEICNGIDDNCNGSIDEGLTFTT
                     YYADADGDGYGDPGTSVTSCAPVAGYVADNTDCNDASGAAYPGATEVCDGIDNDCDGD
                     IDENVVTAVMSPTGDVTTCKSDDFVFSVEPCAGCTYQWFKNDNVIVGATGTTYATTKP
                     AYYNVQVTTAAGCFDVSDYALLTTLLNPNANIYNPNGLNLCAPTPGTNILIKVGYTAT
                     NTYQWYKDGLPYTGDGATSWRIYPTETGDYYCAITGASGCYRETEVRTVINACRMADV
                     ADQGVNIYPNPASNKVTVSLSLVSSAEYADITIVNMMGQSLATTTTAMSDGMADVTIN
                     TADLPAGMYTVIVRAGDQINMNELMIVK"
BASE COUNT         1559 a         1056 c         1032 g         1463 t
ORIGIN      
        1 agtaatcgag tgtttagcag gctcaaatat ctatgctcaa gtccagtttc tggatcaaaa
       61 gaacggcatg tggaatgctt ccttatgttt gttgaaacat tatgaatcaa aggcagatac
      121 aatattgaat gctgaagaag catttaaatt ctatcgttat tattttgaat ggatgatgag
      181 tattgccaat gcaatagcat ctgatgcaga catagataaa ctggcgaaaa aactattgtc
      241 taaatgtccg gctgtttcct ataatgaaac gcagttatgg ataaagattg tgttattcag
      301 aaaggacatt aatgttacta caacacgagc cgattacacc aatccgaaga tagccgaagc
      361 attgcagtcg cgcttacatg atttgattta cgaagccgat gccattaatt ctgtttattg
      421 tttatataag gcaaagctta ccggaatatt tctgtgcaac ctcacgcaga atttctctca
      481 gagcagaatt tacatcagcg atatcaatga attatttgca caatcgccgg gagcatttac
      541 agaatcggaa ttgttgactg cacaatggca ttatgcgcag atattatttt ttagcagtca
      601 gtttgatgaa agctttgtga tctatgaaca attgtttcaa aagatcaatg tttctgaaac
      661 catgcgctgg tatgtattca ttgccgaatt cttccagaac tgtctggtaa ccggacacta
      721 tgaccaggca cagcaacttt gcacggatta ttttcaaaaa ttctttgacg ataaaaacgg
      781 aagcttttat ttatcagcat taatacaatg cgtaaaattg cttattcaca caagtgattt
      841 tgaaaaggca aaattaaaat tggatgaatt aaataagctg acaacaaaaa cttcatcatt
      901 acaatttcaa tttgccttgc gtgaattaac aagcgcctat tactatttga ccggaaacta
      961 caaggaggca cttatattag ctgagaagaa tttaaaattc atgcggtcga aacgcatcca
     1021 ttacctgatt cctgagtaca ccttccattc acgcttaata aaagcactta ttaagcaaaa
     1081 gcagcgtcag aaatctttta ctgaagaaga ggtgaaaatg atggatgcaa tgcaacaagg
     1141 cactatggcg caatatggct tattgttaaa aagacttgaa aaaaatctta actactttat
     1201 agtataaaaa aatggactgc ctaacataag cagtccattt tttattgtat gctgaaatgg
     1261 ttgaaagtat ttattaccgg caaccgcgca ttttaattct gaattactat tcttgattca
     1321 gctattgtat tattttgtat aacgattaca gaatacattc cggcaggaat aacacctgta
     1381 agatcaagtg tttgatctaa ttcgaaattg ctgtaatctg caacagtaga ataaactatt
     1441 tcaccaagca tatttacaat tgtaattgat gccgttcctt ccttagctgt tgtagataaa
     1501 ttcaaatgca attgacctga ggtaggattt ggataaacct ccagattatt atttgcggtt
     1561 gcattttctt cgcggcatgc ggtaaccgta atttctgctg tattacgtga gcaaccaaaa
     1621 gcattagtaa ctatgcaata ataatttcct gccattgttg gaaaatattg atacgccgta
     1681 gcgcctgcaa tttcaccggc accatcgtac cattggtagg tatacgaggc atcatagctg
     1741 gctttcagtt taacggtagc acataagctt gttccgtttg gtgcactgat atttgcattc
     1801 ggatttggcg aaaccgtaat tgtggtaaca ggtgacaatg caaaacatcc ttcaggaaga
     1861 tttacctgta cttgataata gccgggttta tttgaagaaa aagttacgtc ggtagcaccg
     1921 gcaataatat ttccattttt aaaccactga taggtataac tttcgcctgc atttgcagtg
     1981 agggtagtat tcactccttt acaagtagaa actacacctg ccggagtgat ggtagcagat
     2041 accacgtctt catcgatatc accatcgcaa tcattatcga gtccatcaca aatttccgtc
     2101 gctcccggat aaacggtagc tgctgcatca ttacaatctg ttgcatccat tacgtatcct
     2161 acaggagccg ggtcgcaggt aaagaacgtt gaagctgcat ctccatatcc atcgccatcg
     2221 gaatcggcgt accaagtagc aaagatgagg tcttcatcaa tattgccatc gcagttatca
     2281 tctataccgt tacaaatttc agttgccaga atatttacac cggcattggt atcatcacaa
     2341 tcgcccggat caatagagta tccggttggc atattacaag cgaatacaaa tacatcagaa
     2401 tttccgaatg tatcgccatc taaatcttca aaccacaatg gcaaaccttc agcagaagga
     2461 tcgtcatcat ctaccagtcc gtcgcaatca tcgtcgtacc cattacatac ttctgtagct
     2521 gcgggattaa tcatagcatc agcatcatta caatcttcat cattgccaac aaatcctgtc
     2581 ggagcaacgc aggctacagc ggtggttgca ggatcgccat ataaatcgcc gtcggcatct
     2641 gcaaaatagg taaccatggt accttcatcg atcaaaccgt cgcagtcgtc gtcataaccg
     2701 ttacaatttt caggagcacc cggatatact aatgcgttag catcgttaca atccgtaacc
     2761 actgaatatc catctccatc agcatcaggt tgaacgctga tattatcaat agcaaaactt
     2821 ggacgagaac cgccgccggt catatcgcgg gaaatccagc gaacttgaac ttctgcaacg
     2881 ttatcagttg catcagggag taaaatagaa cgcgacaacg gattctgagg tgttgtaaca
     2941 cccgaaccgg tttgcaaagt ggttccgctt tgatattcga tacctgtcaa tgtgatccat
     3001 gcgccggtat ttccaattct gtattgaaca gttgcttcat tcaatcttgt gttggtagtt
     3061 ccatcatatg gatttcgaat taccatgata tcgtaactca atagcaagtt gctgtaacct
     3121 gtggtattta atgaaagcac tatccccgga tttgtagaac cggaagcgag caatccaatt
     3181 ttaccattgt aattgtgcac gccaccacta ttattggaag cgcttgatga ggcaataagt
     3241 gcctgatcgc cggttgggcc ggttgtaaca aaagaggtag tagcagcaga actacatttc
     3301 caaccttgaa taccggcagg ataagtagtt gatgcatgtg ctaaaccaga aaaatcttgt
     3361 gtgtaaggaa cagcctgtgg tgtggggtta gtttgtgcct gaataataat tggactacta
     3421 agtagcatta aagacaagca aagttgaatg gattttcgta gtaactgttt catatagccg
     3481 gtttttaaat gaattacggt gcaaagaaac ggtacacttg tgtaggtttt ggcgaaaaag
     3541 aattaaacct atgttaataa atggccgaat acatagtgtt ttaacatgta caatattggg
     3601 tttaggttta aaataggcaa aaaaaaaccg cccgataggt ctaccgggcg gctcaattta
     3661 taaatttcta attatttaac aatcatcaat tcgttcatat taatttggtc accggcacga
     3721 acaattaccg tatacatacc tgcaggcagg tcggcagtat tgatcgttac atcggccata
     3781 ccatctgaca ttgcagtggt agttgtagca agtgattgac ccatcatatt tacgatggtg
     3841 atatcagcat attcagctga acttaccaat gaaagcgaaa cggttacctt gtttgaagca
     3901 gggtttggat atatgttcac gccttgatcg gcaacatcgg ccatacggca tgcattgatt
     3961 acggtgcgca cttcagtttc acggtaacaa ccggaagcac ctgtaatagc acaatagtaa
     4021 tcgcctgttt ctgttggata aatcctccaa gatgtagcgc catcgcctgt gtaaggcaaa
     4081 ccatctttat accactgata agtatttgta gcggtgtatc caactttgat caggatgttg
     4141 gtgcccggag ttggtgcgca aaggttcaga ccattagggt tgtaaatgtt tgcgttaggg
     4201 ttcagcaatg tagtcaacaa tgcataatca gatacatcga aacaacctgc agcagttgtt
     4261 acctgcacat tgtagtaagc aggttttgta gtggcataag ttgtacctgt agcacctaca
     4321 ataacgttat cattcttaaa ccattgatag gtacatcctg cacatggttc aacagagaat
     4381 acaaaatcat cgctcttaca tgttgtaaca tcaccagttg gagacatcac agcagtaact
     4441 acgttttcgt cgatatcacc atcgcaatcg ttatcaatac catcgcaaac ttctgttgct
     4501 cctgggtatg cagcacctga agcatcgtta caatcagtgt tgtcagcaac ataacctgct
     4561 accggagcac aagaagtaac tgaagttcct ggatcaccgt atccatcgcc atcggcatcg
     4621 gcataatagg tagtgaatgt aaggccttca tcgatagaac cattgcagtt gtcatcgata
     4681 ccgttgcata tttcagccat tccgggattg atagatgcat tagcatcatt acaatcagtg
     4741 ttatcagcaa cataaccaac aggagcgttg caggctacgg tacttaccgc agcattaccg
     4801 tatccatcgc catcagcgtc ggcataccat gtagtaagcg cgcaagttga accgcttaca
     4861 acaccgttat agatgcgtga ctgtccgttt acgttattag cattccagcc ataaatacga
     4921 atcaataccg ggctggctgt agtaaaatca ggaagatccc aggtaaaggt agttccagaa
     4981 ccacaagacc cgcttggaat agaataatcg ctaccattgt ttgtccatga caaaccgcca
     5041 ttcgtgctat acgcataacg tttcgggtat tgccgaagtg ggggcatttg aaaaccgtca
     5101 gctgaaatat
//
LOCUS       DAOQOU010000205         5092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10119_length_5092_cov_5.256700, whole genome shotgun sequence.
ACCESSION   DAOQOU010000205 DAOQOU010000000
VERSION     DAOQOU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10119_length_5092_cov_5.256700"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PLM90_13110"
     CDS             <1..1512
                     /locus_tag="PLM90_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90369.1"
                     /translation="IDNGCGTDEFALTISAADFNPAGDTIWFKMVNTGAGGLSDMSDH
                     FVYGLWNGALGVDIQSQIHYQTATDWSNVPSGQGIMHGENFKPNGNPTQLSGDWDAMY
                     FYYDIDQSVPSSQSVLYDYTKYLWSDESIRGFRLDAVKHFPAWFVGDLLDDLHDNGMD
                     PDMVVGEYYDYSPSALKGWVDNTLSSMDDDTRAAIHPRIFDFALRNSLEQACDAFGYD
                     VRNVFNASIVDGAGGSGFNVVTFINNHDFRDAGQPVDNDPELAYAYIMTNNKLGVPCV
                     FFPDYYAPGNLKYIINGLMEAHERYIFGATQVDYLSRFSSPYSANYISGGANTSLIYQ
                     TSYAISGREVVVAINFSGNTLKVDQTINTANIHAGDTLTDIFSQTIYPTITVQGDNQI
                     YLEIPPRTFAVYVEGDLRDELIDISTPVAISNYQAQQQFAIFPNPANNDIYFSPLTQN
                     ALLEVYDMAGKRLLQASAGIGDNHLDISMLPSGTYLLKMIAQDNMAIAKFIRQ"
     gene            1514..3142
                     /locus_tag="PLM90_13115"
     CDS             1514..3142
                     /locus_tag="PLM90_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HMZ90370.1"
                     /translation="MTTTQSAFAPIFSSWETVPENARLQFPIHQSQYLLNGTLKNWEG
                     NFTKVISPIYVRTGDTLERVVIGEIPHFTVSEAMLALDAAAKAYDLGRGEWPTMSVKK
                     RIECLITFTEKMKTKREEVVRLLMWEIGKSRADSEKEFDRTVLYIYDTIDALKELDRT
                     SSRIQKEEGIYAQIRRGPLGVVLCMGPYNYPLNETFSTLIPALIMGNTVLFKPAKYGV
                     LLINPLQECFRDSFPAGVVNMLYGDGKEIVTPIMESGKVDVFAFIGTSRVANIIRKAH
                     PKPNRLRACLGLEAKNPAIILPDADLEIAATECITGSLSFNGQRCTALKMIFVHEQIA
                     DAFIKLYTEKLGKLKYGMPWDEKVMLTPLPEENKPAYLNNLIADAKEKGASIVNEHGG
                     EQYLSFCYPTVLFPVNENMRVWHEEQFGPVIPIATFSDINEPIAYIQNSNYGQQCAIF
                     GTDHDKMAQLIDPLVNQVCRLNVNSQCQRGPDKYPFNGRKDSAEGTLSVTDALRVFSI
                     RTTVAFKDSGINREIVNDILDNRKSNFLSTDYIL"
     assembly_gap    3252..3351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3527..5008
                     /gene="malQ"
                     /locus_tag="PLM90_13120"
     CDS             3527..5008
                     /gene="malQ"
                     /locus_tag="PLM90_13120"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HMZ90371.1"
                     /translation="MNKRASGVLLHPTSLPGNYGIGSLGAAARSFVDFLVSARQTYWQ
                     ILPLGPTGYGDSPYQCFSSNAGNPYLIDLDTLVEEGFLSASDIPAWHDADMHKVDYGK
                     SIDFKMAVLKTAFSKFNSEDHPDYKSFTEKNASWLDDYNLFMALKEKFDKRPWYEWED
                     NYRLRDEATIEQAIIELQEGIAFNSFIQYLFFKQWSAIKKYANDHKIQIIGDIPIYVA
                     MDSSDTWCSPELFQFDENKTPVCVGGCPPDYFSETGQLWGNPIYDYAKMEEDGFAWWI
                     NRIKSSLELYDLIRVDHFRGFAGYWSIPYGAPTAVTGQWVDGPGKKLFQAIQDALGEI
                     PIIAEDLGLITPDVIELRDHFALPGMKILQFAFDSSEANDYIPHNYTKNCVVYTGTHD
                     NDTALGWFNTAKEDDKRYMMLYLNSDGHDVCKDLIRAAWASVANTAIVPMQDLLRLGN
                     SARMNLPGSTENVWLWRMGVNDLRNELAEELMGLTMLYARNRG"
BASE COUNT         1476 a         1108 c         1048 g         1360 t
ORIGIN      
        1 atcgataacg gatgcggaac agatgaattt gcactcacta tttctgctgc cgattttaat
       61 cctgccggcg ataccatctg gtttaaaatg gtaaacacag gcgcaggcgg attgtctgat
      121 atgagcgatc attttgtata tggtttgtgg aatggcgcat tgggtgtcga tattcaatcg
      181 cagatacatt atcaaactgc caccgactgg agtaatgtgc caagcggaca aggtattatg
      241 catggtgaaa attttaaacc caacggaaat ccaacacaat taagtggcga ttgggatgcc
      301 atgtattttt attatgatat cgaccaatcc gtaccttcat cacaatctgt tttatacgac
      361 tacacaaaat atttatggtc tgatgaatcc attcgcggat ttcgcctcga cgctgtaaag
      421 catttccccg cctggtttgt aggcgatctg ctcgacgatc tgcatgacaa cggtatggat
      481 cccgatatgg ttgttggcga atattatgat tatagtcctt cagcattaaa agggtgggtg
      541 gataacactt tatcaagcat ggacgatgat acacgcgctg caattcatcc acgtattttc
      601 gatttcgcac tgcgcaatag tttagaacaa gcttgcgatg catttggata cgatgtgcgc
      661 aatgtattta atgccagcat agttgatggc gcaggaggaa gcggatttaa tgtggtcacc
      721 ttcatcaata atcacgattt ccgcgatgcc ggtcagcccg ttgataatga tcctgaattg
      781 gcatatgcct atatcatgac caataataaa ttaggggttc catgtgtgtt cttccccgat
      841 tattatgctc ctggaaattt gaaatatatc atcaacggtt taatggaagc acatgaacga
      901 tatatttttg gtgccactca agttgattat ctgagcagat tttcatcacc ctactccgca
      961 aattatatca gcggcggcgc aaatacctca ttgatatatc aaacaagtta tgccatcagc
     1021 ggtcgcgaag tggtggtagc aataaatttc tctggaaata cattaaaagt cgatcaaacc
     1081 atcaatactg caaatattca tgcaggagat acgctgactg atattttcag tcaaacaatt
     1141 tatcctacta ttacagtgca gggcgataat cagatttatc ttgaaattcc accgcgcact
     1201 tttgccgtgt atgttgaagg agatcttcga gatgaattaa ttgacatctc tactccggtt
     1261 gctatcagca attatcaagc tcagcagcaa ttcgctattt tccccaaccc tgcaaacaat
     1321 gatatttatt tttctccgct cacacaaaat gcattattag aagtttatga tatggccgga
     1381 aagcgccttc tgcaagcatc cgcaggcatc ggtgataatc atctggatat tagcatgtta
     1441 ccttcaggta cttatttact caaaatgatc gcacaagaca atatggcgat cgcaaaattt
     1501 atcaggcaat aatatgacaa caacgcagag tgcatttgca cccatcttct catcatggga
     1561 aacagttccg gaaaacgcaa gattacaatt cccgatacat caatcgcaat atttattaaa
     1621 cggcacactt aaaaattggg agggcaattt tacgaaagtg atttcaccaa tttatgtccg
     1681 cacaggcgac acattggaac gtgtcgtaat tggcgagata ccacatttta cagtgagcga
     1741 agccatgttg gcactcgatg ccgccgcaaa agcctatgac ctcggacgcg gtgaatggcc
     1801 taccatgtcg gtgaaaaaac gcatcgaatg cctgatcacc ttcaccgaaa aaatgaagac
     1861 aaaacgcgag gaagttgtac gcctgctcat gtgggaaatc ggtaaatcac gtgccgattc
     1921 tgaaaaagaa ttcgatcgta cggtattata tatttatgat accatcgatg ccctcaaaga
     1981 gctcgacaga acatcatcgc gcattcaaaa agaagaaggt atttatgcgc aaattcgtcg
     2041 cggtccgctg ggtgtggtat tgtgtatggg accatataat tatcctttga atgaaacatt
     2101 cagcacattg attccggcgc tcattatggg caataccgta ttatttaagc ctgcgaaata
     2161 tggtgtgtta ttaattaatc cactgcagga atgttttcgc gatagcttcc ctgccggcgt
     2221 tgtaaatatg ttgtatggcg atggaaagga aatcgtaaca ccgattatgg aatccggaaa
     2281 agtagatgtg ttcgcattta ttggaactag tcgcgttgcc aatattattc gcaaggcaca
     2341 tccaaagcca aatcgcttgc gcgcatgcct aggattagaa gcaaaaaatc cggcaattat
     2401 tttacctgat gccgatctgg aaatcgcagc tacagaatgt atcacaggct cactttcatt
     2461 caatggtcag cgatgcactg cattaaaaat gatatttgtg catgagcaaa ttgcagatgc
     2521 cttcatcaaa ttgtataccg aaaaactcgg caaattaaaa tatggaatgc cttgggatga
     2581 aaaagtgatg ctcacgccgc ttcccgaaga aaataaaccc gcttatctca ataatttaat
     2641 agcagatgca aaagaaaaag gagcaagtat tgtgaatgag catggcggcg aacaatattt
     2701 aagtttttgt tatccgacag tattatttcc ggtgaatgaa aatatgcgag tatggcatga
     2761 agagcaattc ggaccggtaa ttcctattgc cactttcagc gatatcaatg agccgattgc
     2821 ttatattcaa aacagcaact acggacaaca atgcgccatt ttcggcaccg accatgataa
     2881 gatggcacaa ttaattgatc cgctggtaaa tcaggtatgc cgattaaatg tcaactccca
     2941 atgccagcgc ggacccgata aatatccttt taatggcaga aaagattccg ccgaaggaac
     3001 attaagtgta accgatgcac tccgcgtttt cagcatccgc accacagtag ccttcaaaga
     3061 ctccggcatc aaccgtgaga tcgtgaatga tattttggat aatagaaaaa gtaatttttt
     3121 gtcgacggat tatatattgt aataactgat aattaatcca taagattcaa agattcgaag
     3181 atttgaagga cagaagggaa ggtttttaag ttgaacaaca ttcctattcc ttttttaacc
     3241 cttcgaatct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggaaggttt
     3361 ttaagttgaa caacattcct attccttttt taacccttcg aatcttcaat ccttcaatcc
     3421 ttcaaatctt caaatcttcg aatcttcgaa ccttcgaatc ttcaaacctt taactcttcg
     3481 aatcttcaat ccaaattacc ttactttgca ttgcaattaa tacaatatga acaagcgcgc
     3541 ttccggagtt ctcctccacc ccacatccct ccccggtaat tacggtatcg gttcacttgg
     3601 agccgcagcc cgcagctttg tagatttttt ggtgagcgcg cgtcaaacgt attggcagat
     3661 attgcccctc ggaccaacag gctatggcga ttcaccctat caatgtttct ccagcaatgc
     3721 cggaaatccc tacctcatcg atctggatac tctggtggaa gaaggctttt tatccgcttc
     3781 agatattcct gcctggcacg atgccgatat gcataaagtt gattatggca agagcataga
     3841 ttttaaaatg gcagtgctca aaacggcctt ctcgaaattt aattctgaag accatcccga
     3901 ttacaaatcc ttcactgaaa aaaacgcaag ctggctcgac gattataatc tcttcatggc
     3961 gttaaaagaa aaattcgata agcgtccgtg gtatgaatgg gaagataatt atcggctgcg
     4021 cgatgaagct accattgagc aggcaattat agaacttcaa gaaggaatcg ctttcaatag
     4081 ttttattcaa tacctctttt tcaaacagtg gtcagccata aaaaaatatg ccaatgatca
     4141 caaaattcaa atcatcggcg atattccaat ttatgttgcc atggacagca gcgatacctg
     4201 gtgcagtcct gaattatttc agttcgatga aaataaaaca cctgtttgtg taggcggatg
     4261 tccacccgat tatttcagtg agaccggaca actatgggga aatccgattt atgattatgc
     4321 gaaaatggaa gaagatggtt ttgcctggtg gatcaatcgc attaaatcaa gtctcgaatt
     4381 atacgacctc attcgcgttg atcacttcag aggttttgca ggatattgga gcattcccta
     4441 tggcgcacca acagcagtaa ccggacaatg ggttgatggt cccggaaaaa aattatttca
     4501 ggcaattcaa gatgcgctcg gagaaattcc catcattgcc gaagacctcg gcctcatcac
     4561 ccccgatgtc atcgaactgc gcgatcactt cgccctgccc ggcatgaaga tcctccagtt
     4621 cgccttcgac agcagcgagg ccaatgatta catcccacac aactacacaa aaaattgcgt
     4681 ggtatacacc ggtacccacg acaacgacac cgccttaggt tggttcaaca ccgcgaaaga
     4741 agatgataag cgctacatga tgctctatct caacagcgat ggtcacgacg tatgcaaaga
     4801 cctcatccgc gccgcctggg catcggtagc caacaccgcc atcgtgccca tgcaagacct
     4861 cctgcgcctc ggcaactccg cccgcatgaa cctccccggc tccaccgaaa acgtatggct
     4921 ctggagaatg ggcgttaatg atcttcggaa tgagctggca gaggagctaa tggggttgac
     4981 gatgctttac gcacgcaata gaggctgatt cagacaatga ttgtgtttgg gttcgggtgt
     5041 tcgggttcgg gttaattgtg gaatgtgctt acaccggcaa tccgtgcaat cc
//
LOCUS       DAOQOU010000206         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10150_length_5081_cov_5.612018, whole genome shotgun sequence.
ACCESSION   DAOQOU010000206 DAOQOU010000000
VERSION     DAOQOU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10150_length_5081_cov_5.612018"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..354)
                     /locus_tag="PLM90_13125"
     CDS             complement(133..354)
                     /locus_tag="PLM90_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HMZ90372.1"
                     /translation="MYWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVIENLQELE
                     DEGEFYEGIEDIWSDYPTHDDFYFNEDEY"
     gene            complement(517..1065)
                     /locus_tag="PLM90_13130"
     CDS             complement(517..1065)
                     /locus_tag="PLM90_13130"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMZ90373.1"
                     /translation="MKIYTKKGDEGQTSLIGGTRVPKHHLRVEAYGTIDELNVCIGLI
                     LDQQMHEDLRNVLRTIQDRLFTVGAALASDPERSKMSIPDLVDADVKQLEDAMDAMDA
                     ELAPLQNFILPGGHITVSYCHLARVVCRRAERIVTHLAENEHVEPIILIYLNRLSDYL
                     FVAARKIAHDLGIDEVAWRPRG"
     gene            complement(1091..1819)
                     /locus_tag="PLM90_13135"
     CDS             complement(1091..1819)
                     /locus_tag="PLM90_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ90374.1"
                     /translation="MSDIITLSNIRKTYDLGKMRIEVLKGLNTTIAKNEYVAIMGPSG
                     SGKSTLMNILGCLDKPTKGQYVLNGTDVSRMNEDQLATVRNEEIGFVFQTFNLLARLS
                     GIENVALPLIYAGIKRNERVERATAILQAVGLGHRMHHKPNELSGGERQRVAVARALV
                     NNPSIILADEPTGNLDTKTSYEIMELFEDIHAKGNTVIIVTHEEDIARYAHRIIRIRD
                     GVIERDEVNANKLNPTELRKNLVS"
     gene            complement(1822..2112)
                     /gene="gatC"
                     /locus_tag="PLM90_13140"
     CDS             complement(1822..2112)
                     /gene="gatC"
                     /locus_tag="PLM90_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HMZ90375.1"
                     /translation="MKVDKQLIDKLANLSKLEFSPEEAAAIEKNLSDMLDFVDKLSEV
                     DVSGVEPLVYVNPAINVMRPDEPQMDISKEDALKNAPLADSDYFKVPKVIRK"
     gene            2205..3206
                     /gene="trpS"
                     /locus_tag="PLM90_13145"
     CDS             2205..3206
                     /gene="trpS"
                     /locus_tag="PLM90_13145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMZ90376.1"
                     /translation="MKIVVSGTRPTGILHLGNYYGAVRNYVRLSKDSDVRGYFFIADY
                     HSFTTHINPTELPTLVRQTICTYLASGLDPEKVIIYVQSDVPQIAELYLLLNMLAYKG
                     ELEKVATFKEKIKLHNENINAGLLTYPVLMAADILIHRAYMVPVGKDQEQHLEMTRDY
                     AKRFNNRYDVDFFPEPIAYNFGEELVKVPGLDGSTKMSKTGGETNAIFLNEEPDMIRK
                     KIMRAKTDSGPTEMNSKPSQEIQNLFELMQIVSAEDVVSHYKNAWDTCTIRYGDMKKQ
                     LAEDMVNYLSPFREKINALMQDTDYLGKIVRRGGEQARESAEKTVSEVRKIMGINYF"
     gene            3263..4072
                     /locus_tag="PLM90_13150"
     CDS             3263..4072
                     /locus_tag="PLM90_13150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ90377.1"
                     /translation="MSKSNTPSNKSKAAKSGSTAKPKKAAEKPEKKAAKKPVATVKEA
                     PKKTVKPVPVAKTKPEGGRIVRHVAIAGNIGAGKTTLTHLLAKHFGWETHFEDVENNP
                     YLYDFYQDMPRWAFNLQIYFLNSRFKQILDIQKGDKTIIQDRTIYEDAQIFAPNLHAM
                     GLMSTRDFQNYTEIFNSITSLVSPPDLLIYLRASIPALVNQIQKRGREYEDNLRLDYL
                     RRLNEYYEAWISKYTHGKLLVIDIDQCNFAENPEDLGDVIQRVQTELYGLF"
     gene            4075..>5081
                     /locus_tag="PLM90_13155"
     CDS             4075..>5081
                     /locus_tag="PLM90_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ90378.1"
                     /translation="MEFPVDRYRLDTLGCNDTIHFNNAGCSMHPMQVVESICNYLMEE
                     ALTGGYELAHQRASLLNDFYNLGAEMLHAAPNNIAFVSNATDGFAKALSSIPWHTGDV
                     ILTTINDYVSSQIQFASLHKRFGVEVVRAANTPQGDVDIDSIETLIKQYQPRAISITH
                     IPNNTGMIQPVEQIGDLCEKYNLWYFVDACQSAGQIPLDVTSIKCDFLSFSMRKFLRG
                     PRGSGMLYVSDKALKAGLEPLFIDMRGADWTEANKYQPVPDAKRFEYVEQSYGIITGA
                     IAAMRYYLNVGPDQIALYNAELCAYARQQLSAIPHIHLQDRGSRLSAIISLTSDRSDP
                     M"
BASE COUNT         1487 a         1064 c         1064 g         1466 t
ORIGIN      
        1 gcatacaagg tggcaatgaa agaaaaatac cgctttttca gttatggtga tgccatgctg
       61 attgttgact aagagatatc ctcaaaaaaa aatcctgccg atgagcagga tttttttttt
      121 gtttcaaccg gatcaatatt catcctcgtt gaaataaaag tcgtcgtggg taggataatc
      181 actccagata tcctcgatac cttcataaaa ttctccttca tcctctaatt cctgcagatt
      241 ctcgatcact tcaataggtg ctcccgaacg aatagcatag tcaatcaatt catctttggt
      301 ggcaggccat ggtgcatctt caagatatga ggctagttcc agtgtccaat acataattaa
      361 gtccgcgatt ttattagggc gcaaaaatat accttttttc aggttttaca acttgtattg
      421 taaacaatct gtgcgtcaaa gcgaaaaatc gctgaaatgc ttaccagtaa agggttacag
      481 gattgaaaaa atatctgtat atacaagaat acagcactat ccccgcggtc gccatgctac
      541 ttcatctatt cccagatcat gtgcaatttt gcgtgctgcc acaaaaagat agtcgctcaa
      601 acggtttaag tagataagga ttattggttc aacatgttca ttttcagcca gatgcgtaac
      661 aatacgttct gcgcggcgac aaacaactct agccagatgg cagtatgaaa ctgtgatatg
      721 tcctcccgga agaataaaat tttgaagtgg ggccaattca gcatccatag cgtccatggc
      781 atcttcaagc tgcttcacat cagcatcaac taaatcggga attgacattt ttgaacgctc
      841 cgggtcagat gccagtgcag ctccaacggt aaataagcga tcttgaatgg tacgcaaaac
      901 attgcgaagg tcttcatgca tctgttggtc caaaatcaat cctatacaaa cattcagctc
      961 atctatagta ccataagcct cgacacgaag gtggtgtttc gggacgcgag tgcctccgat
     1021 cagtgaggtt tgtccttcat cacccttttt agtgtagatc ttcatgtttt ccggtaaaat
     1081 ttagtagtta tcaggatacc aaatttttac gtaactctgt gggattcagc ttgttagcat
     1141 taacttcatc acgttcgatc acgccatcgc gaattcgaat aatacgatgc gcatatcggg
     1201 ctatatcctc ttcatgagtt acaatgatca ctgtattgcc ctttgcatgg atatcttcaa
     1261 acaattccat gatctcataa ctggtctttg tatcgagatt acctgttggc tcatcggcaa
     1321 gaataataga gggattattt actaaagctc tggcaacagc aacacgctga cgttcgccgc
     1381 ccgataattc gtttggtttg tggtgcatgc gatgcccgag tcctacagcc tgcaatatag
     1441 cagttgctct ttcaaccctt tcattgcgtt ttatgccggc gtaaatgagc ggtagagcta
     1501 cattttcaat accactaagc ctggcaagca gattaaaagt ttgaaatacg aatccaattt
     1561 cttcatttct caccgttgcc aactgatctt cgttcatacg gctgacatca gtgccattga
     1621 gcacatattg gcctttggtc ggtttatcca ggcagcctag aatattcatc agggtactct
     1681 ttcctgagcc tgagggcccc ataatcgcaa catattcatt ttttgctatg gtagtattca
     1741 agcccttcag cacctcgatt ctcattttac cgaggtcgta tgttttgcgg atattggaaa
     1801 gcgtaatgat atcgctcata attacttacg gatcacttta ggcaccttaa aataatcgct
     1861 gtcggcaagt ggggcatttt tcagcgcgtc ctcctttgag atgtccatct gcggctcatc
     1921 agggcgcata acattgatag cagggttaac gtaaaccaga ggttccactc cggatacatc
     1981 aacttctgaa agcttatcaa caaaatcaag catatctgaa aggttcttct cgatggcagc
     2041 agcttcttca gggctgaatt ccagcttaga caggtttgcc agtttatcga tcaattgctt
     2101 atccactttc ataaggtaaa ggtaaggcga ttttttttgg caatgatggt cttcacacag
     2161 tttaacggag ggtcaatgcc atatcttttt tttattttcg ccgcatgaaa atcgtggtga
     2221 gtggtacccg gccgacgggt attttacatt tgggcaatta ttatggtgcc gtgcgcaact
     2281 atgtgcgtct gagcaaggat tcggatgttc ggggatattt ctttattgcg gattatcact
     2341 ctttcaccac acatatcaat cccaccgaac ttcctacgct ggtgcgacag acaatctgca
     2401 cttatttagc cagcgggttg gatcctgaaa aggtaatcat ctatgtgcaa agcgatgttc
     2461 ctcaaattgc tgaattatat ctgctgttga acatgcttgc ttataaaggt gaacttgaga
     2521 aggtagccac ctttaaggag aaaattaaac tgcacaatga aaatatcaat gcaggattat
     2581 tgacttatcc tgtgctgatg gcagctgata tattaattca tcgtgcttat atggtaccgg
     2641 tgggaaaaga tcaggaacag catcttgaaa tgacccgcga ttatgccaaa cgatttaata
     2701 atcgctatga tgtcgatttc tttcctgaac ccattgcata caatttcgga gaagaattag
     2761 ttaaagtacc gggattggat ggcagtacaa aaatgagcaa aacgggtggc gaaacaaatg
     2821 ctatttttct gaatgaagaa cctgatatga ttcgcaaaaa aatcatgcgt gctaaaactg
     2881 attcaggccc aacagaaatg aatagcaagc catcgcagga aattcagaat ttatttgagt
     2941 tgatgcagat cgtttctgcc gaagacgtgg tttcacatta caaaaatgca tgggatacct
     3001 gcaccattcg atatggtgat atgaagaagc aactggctga ggatatggtg aattatttga
     3061 gccctttcag agaaaagatc aacgcattga tgcaggatac cgattattta ggtaagattg
     3121 tgcgtcgagg aggtgagcaa gcgcgtgaaa gtgctgaaaa aacagtgtca gaggtgcgaa
     3181 aaatcatggg aattaattac ttttaaccaa ttatgtgtga gtgctgatac aagtgctcat
     3241 cccgaatcat cagaaaccta tcatgtctaa gtccaatacc ccttccaata aatcaaaggc
     3301 cgcgaagtcc ggttctacag ctaagccaaa aaaggccgcc gaaaaaccgg aaaagaaagc
     3361 agctaaaaag cctgtagcaa ctgttaaaga agcgcctaaa aaaacagtga aacctgttcc
     3421 ggtagcaaaa acaaaacctg aaggtggtcg gattgttcgt catgttgcca ttgcaggaaa
     3481 tattggtgcg ggaaaaacca cacttacgca tttacttgca aaacattttg gatgggagac
     3541 acatttcgaa gatgtagaaa acaatccata cctctacgat ttttatcaag atatgcctcg
     3601 ttgggcattt aatttgcaga tatacttttt gaatagccgt ttcaaacaaa tactggatat
     3661 tcaaaaagga gataaaacca ttattcagga tcgcaccatt tatgaggatg cacaaatatt
     3721 tgctcctaac ttgcatgcga tgggtttaat gagcaccaga gatttccaga attatactga
     3781 aatatttaat tccatcactt ctttagtatc gccacccgat ctgctgattt accttcgcgc
     3841 gtcgattccg gcactggtta accaaattca aaaacgcggt cgcgaatatg aagataatct
     3901 tcgcctggat tatctgagaa gattaaacga atattacgaa gcctggatca gcaaatacac
     3961 acatggaaaa ttgcttgtga tcgatatcga ccaatgcaat tttgctgaaa atcccgaaga
     4021 cctaggcgat gtaattcagc gcgtgcaaac cgaactttac ggattgttct gattatggag
     4081 tttcctgtcg acagatatcg ccttgataca ctcggctgta atgataccat ccattttaat
     4141 aacgcgggtt gctccatgca tccgatgcag gttgttgaat cgatatgtaa ttacctcatg
     4201 gaggaggcat tgacgggagg ctatgaatta gcacatcaac gcgcttcact attgaacgat
     4261 ttttacaacc taggtgcaga aatgctccat gcggcgccta ataatattgc cttcgtttca
     4321 aacgcaaccg acggatttgc aaaagcgctt tcctcaatac cttggcatac cggtgatgta
     4381 attcttacta ctattaatga ttatgtaagc agtcagattc aattcgcctc actgcataaa
     4441 cgatttggtg ttgaagtagt tcgcgctgca aatacgccac agggtgatgt agatattgat
     4501 agcatagaaa cattaattaa acaatatcag ccccgtgcga tttcaataac acatattcca
     4561 aacaataccg gcatgattca gccggttgaa caaattggtg atttgtgcga aaaatataac
     4621 ctctggtatt tcgtggatgc ttgccaaagt gctggacaaa ttccattaga tgttacatcc
     4681 attaaatgtg attttctttc cttctccatg cggaaatttc tgcgtggtcc acgtggcagc
     4741 ggaatgttat atgtttctga taaagcactt aaggccggtt tagaaccatt atttattgat
     4801 atgcgtggtg ccgactggac tgaagcaaat aaatatcagc cagttcccga tgccaagcgt
     4861 tttgagtatg tggaacaatc gtatggcatc attacgggag ccatagcagc catgcggtat
     4921 tatttaaatg ttggacctga tcagattgct ttatataacg cggagttgtg tgcctatgca
     4981 agacaacagc tttcagccat tccgcatatt catttgcagg atagaggttc gcgattaagt
     5041 gccattattt cgctcacctc cgatcgcagt gacccgatgt a
//
LOCUS       DAOQOU010000207         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10220_length_5061_cov_5.722133, whole genome shotgun sequence.
ACCESSION   DAOQOU010000207 DAOQOU010000000
VERSION     DAOQOU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_5061_cov_5.722133"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1236
                     /locus_tag="PLM90_13160"
     CDS             196..1236
                     /locus_tag="PLM90_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90379.1"
                     /translation="MHPENKIKMLIKSASRLGSCLIAIISVSLIAHNAADGNNFSIIN
                     IYSNNYHQLPDSLHEDSIETIEGLFDDTITAVEIYVTGDTVGYVIDGLDSIIYTQGDT
                     SGFFIGSGNSESFYVGDADTSTYFTLLNDSLIEIEYADSTSYYVSNEAGAYLIPKGEE
                     IAYPLYKLDTLIEGRTNSGVEIVEISINQNLNDILVSLGHTAKNVNPGLFGVHIGGMF
                     DNLTTPNEVSSEYAWQWLVDLAPTTLRFPQGSGSKFMHLLHYADGSDVTGYGYDIIEI
                     ARYFDYIDGESPFDYTDLSASDINSIQTAVDPSWIPLEYREDFNAYHDKWENQNCEDV
                     DTKFIDDFIALV"
     gene            1339..3195
                     /locus_tag="PLM90_13165"
     CDS             1339..3195
                     /locus_tag="PLM90_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90380.1"
                     /translation="MRAEGVNVVGIEMGNECYLEFFCDVLHFYSFEDYWQFINGTNLL
                     GNDDILYNDPSPSVHTDMIEDHDFITAFKTASTSNNYNYKVGIVGMPLEEGFVFNSPQ
                     ETLYGERVSCNDATIWNPAIYSKYGEHVGTSSKYKFDAVIMHTYYTSDHWRDIVFDNF
                     NPVTSCTSSSDLWKYDIYDSRLQATFNGIIGIGDLPGNFKDFLVNGKDDGLGFASAID
                     RMSYYFKFTEAEPNPKDLWITEWNLKDNYRNGTKLEQTECEIYSNTFAHSFLFYQWWL
                     KQLKVNFDPDYRENFLTYSTVQNYINGTSINLVSVADEQEEDYYSVAYGGADCLQRNY
                     HVRRTTYFATYLLSPIFNEKLKYLRTSNTLPFFPNNSGNIQPTVFIDVTGQYLYIYYT
                     NTKNSIQNYRIAQGTLKQLYPDATSISFDEEGTTNTYILAQQLYSSSGKVTLNKYDKD
                     WEVGDPESEFQGLNACYENYDHEMEIMPTTESGNFPTIHNFGNYPQCSGGNYLTLHNC
                     FTAQPYSIGYFKVHFTPMYPLKLNAANLSELVVYPNPANSKIRVAMTDINTAGVETAK
                     IQIFNIQGEIVFVGLFENYNEYDISTLPSGLFQIIITDFYGNFLKSSFVKVE"
     gene            3224..4114
                     /locus_tag="PLM90_13170"
     CDS             3224..4114
                     /locus_tag="PLM90_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90381.1"
                     /translation="MDINAYASIAAYFLGIPNSGVSQVVYVDLNPDITVDEAAFPFGL
                     DMNSDEIIDFEFLHSAFSFYSAPFNSYRYRIDMLVKPLNSQNAISGSYHYHGTFYGGA
                     WWYYPYALLVNDTINESGIWQVGSAQILGLITIDSDDSFIHYAAAADWFNSEISETTN
                     RFIGVRFIDIENSIHYGWVRCDLINSGNTLIIKDYAYNATNDYGLYAGTEISINSSIA
                     KDEWNIFTNNNILNIHYNGIDFEISKVSIYSSNGELMYNAELSMNFTPIDVSELEHGI
                     YFVVVEDNSKRVTKELFLGF"
     gene            complement(4357..>5061)
                     /gene="cysQ"
                     /locus_tag="PLM90_13175"
     CDS             complement(4357..>5061)
                     /gene="cysQ"
                     /locus_tag="PLM90_13175"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HMZ90382.1"
                     /translation="ATDDFAVTDKSDNSPLTKADKASNAVIVAALQKQYPQIPIISEE
                     NKLLAYDERKGWDTLWLVDPLDGTKEFIKKNGEFTVNIALIQNGKPVMGVVGIPARNH
                     MYYAVEGKGAYKVDEHGNTTKLQIAEPRTDTIALIGSRSHPSPEFDAYLKDMESKYAQ
                     VDFVPAGSSLKFCLVAEGKADVYPRLGPPMEWDTAAGHAVVLEAGARVKVYGSTDDLS
                     YNKENLLNPFFIVEKA"
BASE COUNT         1576 a          907 c          987 g         1591 t
ORIGIN      
        1 cagattttgc aatccaaaga acaattcttt taccctaaaa ggaaaactga attaccgaaa
       61 tagaaaacta tttattacat ttataaaaca aattttaata aggcttcaga attctaaaga
      121 aatcgcccta atttgctttg tctatgtgat tgctcgttaa aacaacattt aaaaaaccca
      181 aacaaaacaa acattatgca ccccgaaaac aaaataaaaa tgctaattaa atctgcgtcc
      241 cgattaggat cttgcttaat tgctattatt tccgtaagtc taattgctca taatgcggcc
      301 gatggaaata atttctcaat tataaatatt tattcgaaca attatcatca actcccagac
      361 tctttgcatg aagattcaat tgagactatt gaaggcttat ttgatgacac gataacggcg
      421 gtagaaatat atgtaactgg cgacactgtt ggctacgtta tcgatggtct tgattcaatt
      481 atttacactc agggtgacac ttcaggcttt ttcattggtt ctggcaattc ggaatccttt
      541 tacgtcggtg acgcagacac ctccacatat tttaccttat tgaatgattc acttattgaa
      601 atagaatatg cagattcaac ttcatactac gtttcaaacg aggctggagc gtatttgata
      661 cctaaaggtg aggaaatcgc ttatccatta tacaaattag acacattgat tgagggaagg
      721 acaaattccg gtgtagaaat tgttgaaata tccattaatc aaaatttaaa tgatatttta
      781 gtaagtttag gccatactgc gaaaaacgtc aatcccggat tgtttggagt ccatatcggt
      841 ggaatgtttg ataatctcac aactccgaat gaagtatcta gtgaatacgc atggcaatgg
      901 ctcgttgatt tggcaccaac tactttgaga tttcctcaag gatcaggaag taaatttatg
      961 cacttgcttc actatgcaga tgggtcagat gtaactggct atggctatga cattatcgaa
     1021 attgctcgat attttgatta tattgatggg gagtctcctt ttgattacac ggatctcagt
     1081 gcttctgata ttaattcaat ccaaactgca gttgacccct catggatacc attagaatat
     1141 cgggaagatt ttaatgcata tcatgataaa tgggaaaatc agaattgtga agatgtcgat
     1201 actaaattta tcgatgattt tatcgccctt gtttaatcaa tagatgccgt tgacccagga
     1261 aggccaaaaa caaaagtcat tctcgactta aatattataa gcgaacctgc cactgagtgc
     1321 agagcgataa cagattattt gcgcgcagaa ggagtcaatg ttgttggaat tgaaatgggc
     1381 aatgaatgtt atctagaatt tttttgtgat gtgctgcatt tttattcttt tgaagactat
     1441 tggcagttta ttaatggaac aaatttactg ggaaatgatg acattcttta caatgacccc
     1501 agtccttcgg ttcatactga tatgattgag gatcatgatt ttataacagc ttttaaaacg
     1561 gcttcaacta gcaacaatta taattataag gttggtattg ttggtatgcc tcttgaagaa
     1621 ggatttgttt ttaattcccc acaggagaca ttatatggtg aacgagtaag ttgcaatgac
     1681 gcaacaattt ggaatccagc aatttattct aaatatggag agcatgttgg cacaagttca
     1741 aaatataaat ttgatgccgt tataatgcac acatattata ctagtgacca ttggagggat
     1801 attgtttttg ataactttaa tccggttact tcttgcacaa gctcttcaga tttgtggaaa
     1861 tatgacatat acgacagtcg tttgcaagct acattcaatg gtattattgg cattggcgac
     1921 ctacctggta atttcaagga tttcttagta aacggcaaag acgatgggct aggatttgct
     1981 tcagcgatag ataggatgag ttattatttt aagtttactg aggctgaacc caatccaaaa
     2041 gacctttgga ttacggaatg gaacttaaaa gacaattata gaaatggtac aaagctcgag
     2101 caaacagaat gcgaaatata tagtaacaca tttgcacatt catttttgtt ttatcagtgg
     2161 tggcttaaac agctcaaagt taattttgac cctgattatc gagaaaactt tctaacttat
     2221 tctactgtcc aaaactatat taatggaaca tcaattaatt tggtttcagt tgcagatgaa
     2281 caagaagaag actactatag tgtcgcctat ggcggcgcgg attgcctcca gcgcaattac
     2341 catgtcagaa ggacaacgta ctttgctacc taccttttga gcccaatttt caatgaaaag
     2401 cttaaatatt tgcgaacttc aaacacgtta cccttttttc caaataatag cggaaatatc
     2461 caaccaactg tttttataga tgtaactgga caatacttgt atatttatta tacaaatact
     2521 aagaattcga ttcagaatta tagaattgcc caaggcacct tgaaacagct atacccagat
     2581 gcaaccagca taagctttga tgaagaaggc acaacaaata catatatctt ggcacaacag
     2641 ctgtattcat cctctggaaa agttacattg aataaatatg ataaagattg ggaggttggt
     2701 gatccagaat cagaatttca aggattgaat gcttgttatg agaactacga tcatgaaatg
     2761 gaaataatgc caacaacgga atctggcaac ttcccaacga ttcacaattt tggcaactat
     2821 ccacaatgtt ccggcggtaa ttatttaaca cttcacaact gttttacggc gcaaccatat
     2881 tcaattggct actttaaggt gcactttaca cctatgtatc ctttgaagtt aaatgccgcg
     2941 aatttaagtg aacttgtagt ttacccaaat ccagcgaatt ctaagattcg tgtcgccatg
     3001 acggatatta atactgctgg cgttgaaaca gccaaaattc aaatatttaa tattcagggc
     3061 gagatagtct ttgttgggct atttgagaat tataatgagt atgatatttc cacactccct
     3121 tctgggttat ttcaaattat cataactgat ttttatggca attttctgaa atcaagtttc
     3181 gttaaagtag aatagagatt tttaaatgaa aataataaaa actttggaca ttaacgctta
     3241 tgcatccata gcggcatatt ttctagggat tccgaattct ggagtatcgc aggttgttta
     3301 tgttgatcta aatcctgaca ttacagtaga cgaagcagct ttcccatttg gattggatat
     3361 gaattcagat gaaataattg atttcgagtt ccttcattcg gccttttcgt tttatagcgc
     3421 tccctttaat tcatatcgat atagaattga tatgttggtt aaacctttaa attcccaaaa
     3481 cgctatcagt ggatcatacc actatcatgg cactttttat ggcggagcat ggtggtatta
     3541 tccttatgca ttattggtaa atgatacgat taatgaatct gggatatggc aagttggctc
     3601 agcacagatt ctaggtctaa tcacaattga ttctgatgac agctttattc attatgccgc
     3661 ggcggctgat tggtttaatt ctgaaatttc ggaaacaacc aataggttca ttggagttag
     3721 atttattgat atagagaatt cgattcatta tggttgggtg cgatgtgatt tgatcaattc
     3781 cggaaacacc ttgattataa aagattatgc ttacaatgca acgaatgatt atggtttata
     3841 cgctggaact gaaatcagta taaatagctc gatagcgaaa gacgagtgga atatatttac
     3901 aaacaataat atcctaaaca ttcattacaa tggaatcgat ttcgaaattt caaaagtaag
     3961 tatatatagt tcaaatggag aattaatgta caatgcggaa ttatcaatga atttcacacc
     4021 aatagatgtg tctgagcttg agcatggaat ttattttgtt gtggttgagg ataattcaaa
     4081 aagagtgaca aaagagttgt ttttaggttt ttaactatag ctcacattta atttaatata
     4141 ttgtctttat tgacattcac ggcaggtcaa atttattcat attaaatatg ctcgccgtta
     4201 agtggaatga aaggagacaa acggaatgac tatagctacg aacggatttt gggtgggagc
     4261 aagtcactcg ctaccggcaa gctagtcggt ttaatgcata caaccccatc aacccgcaca
     4321 agccggctga tgggatctca tatcatcaga gacaacctac gccttctcca caataaagaa
     4381 aggattcagc aaattctctt tgttataact aagatcgtcg gtgctgccgt aaacttttac
     4441 gcgggcgccg gcctcgagca ctacggcgtg gcctgcggcg gtgtcccatt ccatgggggg
     4501 gccgaggcgt ggataaacat cagctttgcc ttcggctacg aggcagaatt tcagggatga
     4561 accggcagga acaaaatcga cctgcgcata tttcgattcc atatctttca gataggcatc
     4621 aaattcaggc gacggatgtg aacgtgagcc gataagtgct atggtatctg ttcttggttc
     4681 tgctatttgc aatttagtag tattaccatg ctcgtccact ttatacgcac cttttccttc
     4741 aacggcataa tacatgtggt tgcgggcagg gatgccaaca acgcccatta ccggtttacc
     4801 attttggatg agggcgatat tcacagtgaa ctcaccattc tttttgatga attctttcgt
     4861 gccatccaat gggtctacca accagagggt atcccaacct ttgcgttcat cgtaggcgag
     4921 taatttattt tcctcgctga tgatgggtat ctgcggatat tgcttttgca atgcagcaac
     4981 gatcactgcg ttagacgctt tgtcggcttt tgtcaatggt gaattatcgc ttttatccgt
     5041 aaccgcgaag tcgtcggtgg c
//
LOCUS       DAOQOU010000208         4964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10535_length_4964_cov_5.495009, whole genome shotgun sequence.
ACCESSION   DAOQOU010000208 DAOQOU010000000
VERSION     DAOQOU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10535_length_4964_cov_5.495009"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(552..1169)
                     /locus_tag="PLM90_13180"
     CDS             complement(552..1169)
                     /locus_tag="PLM90_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ90383.1"
                     /translation="MIISLENISKRFNYEWIFRDVNFAFDENDRCAIIGPNGSGKSTL
                     LKVIAGQLTPSSGNIIYSDTQEIPQENVYQMVSYAAPYLELIEEFTLSEMVQFHFSFK
                     KWKSGLNLQAIISLSGLEKHKHKQLRTFSSGMRQRVKLVLALCAETPLVILDEPTTNL
                     DESGVEWYLHLIRDFGVGRIILVGSNQEREYTFCSKSLNILDFKG"
     gene            complement(1166..1954)
                     /gene="lpxA"
                     /locus_tag="PLM90_13185"
     CDS             complement(1166..1954)
                     /gene="lpxA"
                     /locus_tag="PLM90_13185"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMZ90384.1"
                     /translation="MNQPLAYVHPQAQIADNVVIEPFASISKNVVIGEGTWIGPNVNI
                     MPGARIGKNCRIFPGAVISAVPQDLKYRGEETEVVIGDNTTIRECVTINRGTKYAFRT
                     VVGSNCLLMAYSHVAHDCVIGNNVILANSVNLAGHIEIGDWVILEGLVAVQQFIKIGA
                     HSFIAGGSLVRKDVPPFVKAGREPLSFVGVNSVGLRRRGFAAETIEHIQNIYRLLFVR
                     GYNVTKALQYIEAELPASAERDMIVTFIRNSKEGVMKGFRHINE"
     gene            complement(1951..3357)
                     /locus_tag="PLM90_13190"
     CDS             complement(1951..3357)
                     /locus_tag="PLM90_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HMZ90385.1"
                     /translation="MNKEYQHTIKQSVTVSGVGLHTGKEVDMTFHPAAVNHGYKFRRI
                     DLPGSPVIEADADLVTDTARGTTIAKNGASVSTIEHTLAALVGMGIDNVMIDLNGPET
                     PIMDGSSKEFIEAIHSVGAVEQEAEREYINIAENITFKDANRKTEITVMPADDYQVTV
                     MIDFDSKVLGKQHATLDHISEFSGNIGNSRTFCFLHELEMLYENNLIKGGDLNNAIVI
                     VDKEVTNDEMEKLKKMFNKESVSVKKEGILNNLELHWSNEPARHKLLDVVGDLALAGK
                     PIKGKVIASRPGHPSNVAFAKKLKAYYKLRRTQMVGTHYDPNAEPILDINSISKMLPH
                     RYPFLLVDKIIEMGENHIVGLKNITFNEAFFQGHFPNNPVLPGVLQIEALAQTGGIFV
                     LSKVPDPENYDTYFLKIDKVKFKRKVLPGDSLLLKMELMAPIRRGICEMKATAYVGNN
                     IVTEGELTAQVVKRNPTA"
     gene            complement(3377..4429)
                     /gene="lpxD"
                     /locus_tag="PLM90_13195"
     CDS             complement(3377..4429)
                     /gene="lpxD"
                     /locus_tag="PLM90_13195"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMZ90386.1"
                     /translation="MEFTARELCDFLQGTLEGDPSIKVRKPARIEEGQPGDVCFLANP
                     KYLSFAYTTAASVLIVNADQVFEQPVKSTLIRVKDAYTAIAKVLDQYQNMQRESAAAN
                     HKVESNAIVSPSAKIGAKVHVGSFSTISEDVVVGDNSIIHAQVYLGKRVKIGANTCIF
                     PGVIVLDECVIGDNCIIGAGTVIGSDGFGFAPQADGTYKKVAQTGNVVIEDMVEIGAN
                     TTIDRATMGSTIIRKGAKLDNLIQIAHNVEIGENTVIAAQSGISGSTKIGKNCVIGGQ
                     VGIVGHITIADGTKINAQSGVSKSVKEPDQALTGSPAFEYSAGMRSQVLFRKLPELMD
                     RIKLLEEKISQLENKQ"
     gene            complement(4434..>4964)
                     /locus_tag="PLM90_13200"
     CDS             complement(4434..>4964)
                     /locus_tag="PLM90_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HMZ90387.1"
                     /translation="LIKTLNRAKYLAKSGTQLFATPSLHFFLYRDVATRDFDDDADIL
                     EQYALLDDVDVWASIKEWQHCDDRVLSFLSKSLINRDLFRIELGNEEISASRLADIRA
                     KVQSAYKITDPFDLQYFVFNDVTTNSAYNLEANNINIKFRSGRILDVASASDHLNLAA
                     LADTVVKHYICYPKET"
BASE COUNT         1364 a         1132 c          969 g         1499 t
ORIGIN      
        1 aaggtcggca ttaaaggcag agataatgtt gttggatgaa cctgaagaaa tagcagtata
       61 actgcttgaa ggaacagccc ccatactaat gaaaccgttt gcctgaatac caaaagtggt
      121 atacaccgtt ccattaaatg tgaaattgaa tccgatggga tacgtaccga agttcgaatc
      181 atcggttgca gtggcaacgg ttactgttcc ggcaccgccg tcgatctatg tgaatgtgcc
      241 tgtaaatgag ctaaatccat aagcgcttac ctgtgcattt acactcacta ctgctaacat
      301 acacacgacc aacattaagt gtcgcaggct cctcagccac gacacttttg ttgtgtcaat
      361 gtttcgcata aagtgtgttt aattaaaaaa ttagttctca atgagttcgc cgcccacaca
      421 tgcaggatgc tcctcggtga taaatgtagg gaattggctt tgataccatg caagatttac
      481 accacgaaaa atgacaaaat gatgagaaaa gtaaggaatt gttaaaaaat ggtaaaatcc
      541 gaaagttgga ttcaaccttt gaaatccaga atatttaatg atttcgaaca gaaggtatac
      601 tctctttcct gattggaacc aactaggata attctgccaa caccaaaatc ccgaatcaaa
      661 tgcaaatacc attcaacacc cgattcatcg aggttggtgg taggttcatc taaaattact
      721 aaaggagttt cggcgcacaa tgccaaaacg agcttcaccc gctgacgcat gcctgaagaa
      781 aatgttctta attgtttgtg cttatgtttc tcaagaccgc tgagtgaaat gatggcctgc
      841 aaattcaatc cgctcttcca ttttttaaaa gagaaatgaa attgcaccat ttcagacagt
      901 gtaaattctt ctatgagctc taaatatggt gctgcatagc tgaccatttg ataaacattt
      961 tcttgaggaa tttcttgtgt atcgctataa attatgttgc cgctgcttgg cgttaattgt
     1021 ccggcaatta ctttcaataa tgtacttttt cctgatccgt ttggtccgat aatggcgcag
     1081 cgatcatttt catcaaaagc aaaattcaca tcacgaaaaa tccactcgta attaaatcgc
     1141 tttgaaatat tttcgaggga aataatcatt cattaatatg gcggaaccct ttcatcacac
     1201 cttccttaga gttgcggata aatgtaacta tcatatcgcg ttcggcggac gcaggcaatt
     1261 ctgcttctat atattgtaat gcctttgtta cgttgtaacc acgcacaaat agcaagcgat
     1321 aaatattctg gatatgttca attgtttcag ctgcaaatcc tcttctgcgc agtccaacag
     1381 aattcacacc aacaaagctc agcggctcgc gacctgcttt tacaaatggc ggaacatctt
     1441 tccgcaccaa cgaaccaccg gcaataaatg aatgtgcccc tatttttata aattgctgta
     1501 ctgctacaag accttctaaa attacccaat cgcctatttc aatatgaccg gcaaggttta
     1561 ctgagtttgc gagtatcaca ttatttccga tcacacaatc atgcgccaca tgcgaatatg
     1621 ccattaacaa acaattgctg ccaacaacag tgcggaatgc atacttggtt cctctgttga
     1681 tggtcacaca ttcgcgaata gtagtgttat caccaatcac aacttctgtt tcttctccgc
     1741 gatattttaa atcttgtggc actgcagaaa ttactgctcc gggaaatatg cgacagtttt
     1801 ttccgatgcg tgcaccgggc ataatattta cgttcggacc tatccaggtt ccctccccta
     1861 tcaccacatt ttttgaaatg gatgcaaatg gttctatcac cacattgtcg gcgatctgtg
     1921 cttgcggatg tacgtaggct aatggctgat tcatgctgta ggattacgct ttactacttg
     1981 tgctgtcaat tcaccttcgg ttacgatatt atttcctacg taagcggtgg ctttcatttc
     2041 gcaaatacct cttctgatag gtgccatcaa ttccattttc aacaacaaac tatcaccggg
     2101 taatactttt ctcttaaatt ttactttatc aatcttcaaa aaataagtat cataattttc
     2161 aggatccggc actttactca atacaaatat tcctcccgtt tgcgcaagtg cttcaatttg
     2221 taatacaccg ggtaaaacag gattgtttgg aaaatgtcct tgaaaaaatg cctcgttgaa
     2281 tgtaatgttt tttaatccga caatatgatt ctctcccatc tcgataatct tatctaccag
     2341 caagaaagga tagcgatgtg gcaacatctt cgatattgaa ttaatatcca gtatcggttc
     2401 tgcattcgga tcataatgcg taccaaccat ttgtgtgcgt cgcaatttat aataggcttt
     2461 tagttttttt gcaaaagcaa catttgacgg gtggccgggg cgacttgcaa ttactttccc
     2521 tttaattggt tttcctgcca atgcaagatc gccaacaacg tcgagtaatt tgtgacgtgc
     2581 aggttcattc gaccaatgta attcaaggtt attgagaata ccttcctttt taacagaaac
     2641 ggattcttta ttaaacatct ttttcagctt ctccatttca tcattggtaa cttctttatc
     2701 aacgataaca atggcattat tcaggtcgcc acctttgatg agattgtttt catacaacat
     2761 ctctaactca tgcagaaaac agaaagtccg gctatttcca atatttcctg aaaattctga
     2821 aatgtggtca agtgtggcgt gttgtttacc taagactttt gaatcgaaat ctatcatcac
     2881 cgtaacctga tagtcatcgg caggcatcac cgtgatctct gtttttcgat tggcgtcttt
     2941 gaaggtgata ttttcggcaa tattgatgta ttcgcgttct gcctcctgct caactgcacc
     3001 tactgaatga atagcttcaa taaattcttt cgatgaacca tccatgatag gtgtttctgg
     3061 accattgagg tcgatcatca cattatcgat acccatccca accagggcag caagtgtgtg
     3121 ttcgatagta ctcacactgg cgccgttttt ggcaatggtg gttccacgag ccgtgtccgt
     3181 tactaagtcg gcatctgcct caattaccgg agaaccgggg aggtcgatgc gacgaaattt
     3241 atatccgtgg tttacagccg caggatgaaa ggtcatatcc acctctttac ccgtatgtaa
     3301 tccaacaccg gaaaccgtta cggactgttt gatggtatgt tgatattctt tattcatgtt
     3361 gtcgtgcagt ataacgttac tgcttgtttt ctaattgact tattttttct tcgagaagtt
     3421 tgatgcgatc catcagttca ggtaatttgc ggaacaggac ctgactgcgc atacctgcac
     3481 tgtattcgaa tgcaggagag cctgtaagcg cctgatcggg ctccttaaca gattttgaaa
     3541 caccggattg tgcattaatc ttggtgccat ctgcaatggt aatatgtcca acaatgccca
     3601 cctgaccacc aatcacgcaa ttcttgccga tcttggtgct acctgagatg ccggattgag
     3661 ctgcaatcac cgtattctcc cctatctcta cattatgggc tatctggata aggttatcca
     3721 atttagcccc tttacgaata atggtagaac ccattgtcgc cctgtcgata gtagtatttg
     3781 cgccaatctc caccatatct tctatcacca catttcctgt ttgcgcaacc ttcttataag
     3841 ttccatcagc ttgtggggcg aagccaaatc catctgaccc tattaccgtt cccgcgccga
     3901 tgatacagtt gtcgccaatc acacattcat ccagaacaat cacccccggg aaaatacagg
     3961 tattcgcacc gattttgaca cgttttccca ggtaaacctg ggcgtgaatg atactgttat
     4021 cacctaccac gacatcttca gaaatagtac tgaaactgcc aacatgcact ttggcgccaa
     4081 ttttagctga aggagaaact attgcattcg actcaacctt gtggttagca gcagcgcttt
     4141 cacgttgcat attttgatat tggtcgagca ctttggcaat agcggtatat gcatctttaa
     4201 cccttatcag tgttgacttt acaggctgtt cgaacacctg atcggcattg acaatgagca
     4261 ccgaagcagc cgttgtataa gcaaaggata aatacttggg atttgcgaga aaacacacgt
     4321 cgccgggttg tccctcctct attctggccg gtttgcgcac tttaatggag gggtctcctt
     4381 ccaatgtgcc ttgtagaaaa tcacacaatt cgcgcgcagt gaactccatc cggtcaggtt
     4441 tctttcgggt agcaaatata gtgctttacc acggtatcag ccaaagcggc aaggttcaaa
     4501 tgatcacttg cactggctac atccagtatc ctgcctgacc tgaacttgat gttgatatta
     4561 tttgcctcta gattataggc actattggtg gtaacatcat tgaacacaaa atactgcaaa
     4621 tcgaagggat cggtgatctt gtatgccgat tgcaccttcg ccctgatatc tgccaatctt
     4681 gaagcggaaa tttcctcatt tcccaattct atcctgaata aatcgcggtt gatgagactt
     4741 ttgctcaaga aggataaaac ccggtcatcg cagtgctgcc attcttttat tgaggcccat
     4801 acatccacat catccagtaa ggcatattgt tcgaggatat cggcatcatc gtcaaaatct
     4861 ctggttgcca catcgcgata caggaaaaaa tgcagggatg gcgttgcaaa cagttgcgtt
     4921 ccgctctttg caagatattt cgccctgttc agggttttga tgag
//
LOCUS       DAOQOU010000209         4960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10545_length_4960_cov_6.078287, whole genome shotgun sequence.
ACCESSION   DAOQOU010000209 DAOQOU010000000
VERSION     DAOQOU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10545_length_4960_cov_6.078287"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLM90_13205"
     CDS             <1..762
                     /locus_tag="PLM90_13205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ90388.1"
                     /translation="FSLENNRILGICTALGTAGKNEMPYAIVVLEQMKVRGVFMVFDG
                     VVLIGFRSDDEAIKLAATHLQHSNVQITSAMGTTHEAQQFAAAFDKPIAKERNFLLYG
                     AVDIRKVKVAKGEARFAESGDQAILMQWFELFFHEANLYPPKTKDQIASIKSQQINSR
                     SAMIWMVEGQPVSLAAIIRTTPDIAIIGHVFTPEHARGKGYSASLIYKMCDVFKKRGH
                     GRFGLYVDDENHTAKGVYHRLGFTEVCKCMQIIFS"
     gene            923..2323
                     /locus_tag="PLM90_13210"
     CDS             923..2323
                     /locus_tag="PLM90_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ90389.1"
                     /translation="MHTNEQILQRNIDITLPHDDFYEDLRAREFGRLDTNKQIYLDYT
                     GGNLYALTQLEKHVALLKNNIFGNPHSMNPASAFSTQLVEEARTKVLQFFNAEDYYCI
                     FTPNATGALKIVGESYPFEENGRLLLLADNHNSVNGIREFCITKDGKFDYATIHYEDL
                     MIDADSLDEKLSKPCDCDHKLFAFPAQSNVSGVRHDLSWVTRAQELGWDVLLDAAAYV
                     PTSKLDLRTIQPDFVSISFYKIFGYPTGIGCLLVKKDKFELLHKRWFAGGTVTMVSIL
                     SPHHFLASNHERFEDGTVNYLDIPAISTGLDFIETIGMHRIHARVDGLIQYLTESIAT
                     LQHDNGRPLIRIFGPDHHTHAGGTLIMNVLDINGDQYPFESVERNASDHGISIRSGCF
                     CNPGIDETNYCLTEEELARFYSSRDKGNYADMKLYLGKMRGAIRVSVGYATVQRDIDA
                     FIAFLKTYLNVAVDVP"
     gene            complement(2326..3030)
                     /gene="kdsB"
                     /locus_tag="PLM90_13215"
     CDS             complement(2326..3030)
                     /gene="kdsB"
                     /locus_tag="PLM90_13215"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HMZ90390.1"
                     /translation="MQTFAIIPARYASTRFPGKPLVEIQGKSMIRRVYEQALSANIFS
                     RVVVATDDERIFNHVQAFGGEVVMTSPHHLSGTDRCGEVLASGKYACDVVFNIQGDEP
                     FIQPEQLQLLHKVFENPKAEIATLVKSISDEKDIANPNVVKVVFSKQGQAMYFSRSAI
                     PFARNIGATYYRHIGLYAFRSNILSQLVTLAPTDLERSESLEQLRWLENGFHINVAET
                     QMDTIGIDTPDDLLKI"
     gene            3495..4352
                     /locus_tag="PLM90_13220"
     CDS             3495..4352
                     /locus_tag="PLM90_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMZ90391.1"
                     /translation="MKRICVLILFSLWVWSVTAANPVAGDSVGVKVVDGKSLVVYKLS
                     AGETAYAVSRKYGINFKDLSVANSGLNMDQLKAGQEILVPTGRTANTSTANSNIAKVE
                     TAPREEVVQLKEAVIEAPVVAVPKTPVAETVPEKAEEVATTTEAAAEQTPALSSGDLA
                     ETPATVNNDKSKSFAQIYGGYLDGEHVSKSEKGVATWIESAGIEPSGDRFYALHNSAP
                     IGSVVKVRNMMNNRTIYAKVIGTLSESEVQEKVMIKLSAGAAERLNVLDNRFVSEITY
                     FIPDEQASK"
     gene            complement(4421..>4960)
                     /locus_tag="PLM90_13225"
     CDS             complement(4421..>4960)
                     /locus_tag="PLM90_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90392.1"
                     /translation="IGLQLNYFADTTTSSGLVAEPYYRFYTGSLEDQHRFYLQLSTYA
                     GQVMHTEVYDNPEENVLFESWTNNQDAFTQLFFSLLDMDEHQYITKRFKQNILGAGIS
                     CGFTHRFGNSNWSMYAQAGVKGTSYSTDVPETVVIAETTYTHVEGTIFNSTPQGYTTI
                     DKSRVCNVFTGGLGITYSF"
BASE COUNT         1466 a          991 c         1133 g         1370 t
ORIGIN      
        1 ttttcattgg aaaataaccg cattttagga atctgcacgg cattaggtac tgcaggaaaa
       61 aatgagatgc cttatgctat tgttgtgctc gaacaaatga aggtgcgagg cgtgtttatg
      121 gtttttgatg gggtagtgct gataggtttc agatcagacg acgaagccat taaacttgcc
      181 gccacacatt tgcagcatag caatgttcaa atcacatcag ctatgggcac aacgcatgaa
      241 gcgcaacagt tcgctgcagc cttcgataaa cctatcgcca aggaacgcaa ttttttactg
      301 tatggagctg tcgatattcg caaagttaaa gttgcaaaag gtgaagcgcg atttgcggaa
      361 agtggtgatc aggcaatttt aatgcagtgg tttgaattat tttttcatga agcgaatctc
      421 tatcctccaa aaacaaagga ccagatagca agcattaaat cacaacaaat caatagcaga
      481 tctgcaatga tatggatggt ggaagggcag cctgtaagtc tggcagctat tatccgaaca
      541 acgcctgaca tcgccataat cggtcacgtt tttacacccg aacatgcacg cggcaaagga
      601 tattcggcat cgctgattta caagatgtgc gatgtattca aaaaacgcgg acatggcagg
      661 ttcggattgt atgtagatga tgaaaaccat acggcaaagg gggtttatca tcgcctggga
      721 ttcaccgaag tttgcaaatg catgcagatt atcttttcgt gatggtacca tgacaatgca
      781 tgcgcttgat tgcatgtatt tggattcaac ctttaattca ggtaaaattt attgataatc
      841 gcagggaaac gacggctatt gtgatataag tcatctttac cattatgtat gcatattacc
      901 tttgctctgc caaacgaatg atatgcatac taacgagcaa attttacaac gcaatatcga
      961 catcacacta ccgcacgacg atttttatga ggacttgcgt gcgcgggaat tcgggcgact
     1021 agacaccaac aaacaaattt acctcgatta caccggcgga aatctatatg cacttaccca
     1081 acttgaaaaa catgttgcat tattgaagaa caatattttt ggcaatccgc attccatgaa
     1141 tcctgcttct gctttttcaa cacaattggt tgaagaagca cggactaaag tattgcaatt
     1201 ttttaatgcc gaagattatt attgcatttt cacacctaac gccacagggg cattaaaaat
     1261 tgtaggtgag agttatcctt tcgaagaaaa tggcagattg ttattgcttg ccgataatca
     1321 taattcggta aatggtatcc gcgaattctg catcaccaaa gacggaaaat ttgattatgc
     1381 tactattcac tacgaggatt tgatgataga tgcagatagt ctcgatgaaa aattatcaaa
     1441 gccatgtgat tgcgaccata aattatttgc atttcctgca cagtcgaatg ttagcggtgt
     1501 gcgtcacgac ctgtcatggg taacgcgcgc gcaggaattg ggatgggatg tgttattgga
     1561 cgcagcagcg tatgtgccaa ccagcaagct tgatttgcgc accattcaac ctgattttgt
     1621 gagtattagc ttttataaaa tatttggata tcccaccgga atcggatgtc tgctggtgaa
     1681 aaaagataaa ttcgaattat tgcacaaacg ttggtttgca ggtggtactg tcaccatggt
     1741 atccatcctg tcaccacacc attttttggc ttccaatcac gagcgatttg aagatggtac
     1801 cgtaaattat ctggatattc ctgcaatcag cacaggtctt gatttcattg aaactatcgg
     1861 catgcatcgc attcatgcgc gcgttgacgg cttgatacaa tatctcactg aatcaatcgc
     1921 taccttgcaa catgataatg gcaggcctct catccgaatt ttcggacctg atcatcatac
     1981 acatgcaggg ggaactttga taatgaatgt gctggatatt aatggcgatc aatatccatt
     2041 tgaatcagtg gaacgaaatg caagtgatca tggcatttct atccgatccg gatgcttttg
     2101 taatcccgga atcgatgaaa caaattattg cttaacagaa gaagaactcg cccgttttta
     2161 ttccagccgc gacaagggaa attatgcgga tatgaaattg tatctcggaa aaatgcgcgg
     2221 cgctattcgt gtttctgtag gatatgcaac tgtgcagcgc gatattgatg catttatcgc
     2281 atttctaaaa acctatctga atgttgcagt tgatgtgccg taacattaaa tttttaataa
     2341 gtcgtccggc gtgtcaatac caatggtatc catctgcgtt tcagcaacgt tgatgtgaaa
     2401 tccattttcc agccatcgca attgttccaa cgattcgctg cgttccaaat ctgttggagc
     2461 taatgtaacc agttgactga gaatattact tcggaaggca tataagccaa tgtgacggta
     2521 atacgttgca ccaatatttc tggcgaaagg aattgcgctg cgggaaaaat acatcgcctg
     2581 tccctgctta gaaaaaacta ctttcaccac attcggattt gcgatatctt tttcatcgga
     2641 aatcgacttc actaaagtgg caatttctgc ctttgggttt tcaaacacct tgtgcaataa
     2701 ttgcaattgt tccggttgaa taaaaggctc atcgccttga atgttgaaaa caacatcgca
     2761 tgcatatttt cctgaggcaa gcacttcgcc gcaacggtcg gtgcctgaga ggtggtgggg
     2821 ggaagtcata accacttcgc cgccaaatgc ttgcacatga ttaaaaatac gctcgtcatc
     2881 cgttgctaca actacccggg aaaaaatatt tgccgatagt gcctgctcgt aaacccttcg
     2941 tatcatcgac ttaccctgaa tctccaccaa cggctttccg ggaaatcttg tagaggcgta
     3001 tctggcggga ataatggcga aggtttgcat aattgggtat ggacatatcc ttatgcaggc
     3061 gattaagtgc cttccacaag aacaggtctc tacaggcaaa aataagcgta ttcgctattc
     3121 atttgaagca agcacccgat gtaatgcagt tggaaatgca aggtaatgaa agccggaaac
     3181 aggcaacaaa caacctgaaa ggcttgccgg tattgggttt tgcgcattca taccgttctg
     3241 ctccctccta agcgcttaga agaatgttga gcgatattcc ggaagggtaa aaggctgcta
     3301 tatccacatt ttagcgctaa tagggctgtg tgcataacag tacactgtcg aaatctgcgg
     3361 tgcataacat gtccactttc aataggttag cgcaggttga cgcaattact tttgcatgtg
     3421 aacaaattgt gcataaacaa taataaccct gggagctgag ttatattatg gaagcactgt
     3481 tacaagacaa gaatatgaaa agaatatgtg tcctcatcct cttcagcctt tgggtatgga
     3541 gcgtgactgc tgccaaccct gtagccggcg attcggttgg tgtgaaggtg gttgacggta
     3601 aatcgctggt agtttacaaa ttgagtgccg gtgaaacagc ttacgcggta agcagaaagt
     3661 atggtatcaa cttcaaagac ctgtcggtag ccaacagcgg attgaatatg gatcagctga
     3721 aagcaggaca ggaaattttg gttccaaccg gaagaacagc aaacacatca actgccaatt
     3781 ctaatatagc gaaggttgaa acagctcctc gcgaggaagt ggttcaactc aaagaagcgg
     3841 ttattgaagc tccggtagtt gcggttccga aaacacctgt tgcggaaaca gtgcctgaaa
     3901 aagcagaaga agttgctaca acgacagaag ctgcagcaga acaaacacct gcactttcca
     3961 gcggtgattt ggcggaaaca cctgcaacgg taaacaacga taaatcgaaa tcatttgcgc
     4021 agatatatgg tgggtatttg gatggagaac atgtatctaa atcggagaaa ggcgttgcta
     4081 cctggataga gagcgccggt attgaaccat cgggcgacag gttttatgca ttgcacaatt
     4141 cagctccgat aggatcggtg gtgaaagtgc gcaatatgat gaataacaga actatctacg
     4201 ctaaagtaat aggcaccctg agtgaaagtg aagtgcagga aaaagtgatg atcaaattga
     4261 gtgcaggtgc tgcagagaga ttaaatgtat tggacaaccg tttcgtatcg gagataacgt
     4321 attttatccc cgacgagcag gcatccaaat gatcgttcgt taataagtgt gtgaaagcgg
     4381 tttgttatgc agaccgcttt ttttttgttt attgatacta tcagaatgaa tatgtaatac
     4441 ccagtccgcc tgtaaataca ttacacacgc gacttttatc gatggtggta tatccctgtg
     4501 gtgtagaatt aaaaatggtt ccttcaacat gcgtgtaggt agtttcagca atgacaactg
     4561 tttcaggtac gtcggtggaa taagaagtgc ctttaacacc ggcttgtgca tacatgctcc
     4621 agttactgtt gccaaaacgg tgtgtgaatc cgcatgaaat accggcacct aaaatatttt
     4681 gtttgaatcg ttttgtgatg tactgatgtt catccatatc gagcagactg aaaaataatt
     4741 gcgtgaatgc atcctggttg tttgtccatg attcgaataa aacattttct tcaggattgt
     4801 catacacttc ggtatgcata acctgaccgg cataagtgct tagttgtaaa taaaagcgat
     4861 gttgatcctc aagcgatccg gtataaaaac ggtaataagg ttcggcaaca agacctgaac
     4921 tggttgttgt atcggcgaag taattgagtt gtaagccgat
//
LOCUS       DAOQOU010000210         4882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10785_length_4882_cov_5.650715, whole genome shotgun sequence.
ACCESSION   DAOQOU010000210 DAOQOU010000000
VERSION     DAOQOU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10785_length_4882_cov_5.650715"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLM90_13230"
     CDS             complement(<1..275)
                     /locus_tag="PLM90_13230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ90393.1"
                     /translation="MINTLLERDRIPDFLIRARIRSLLRRRLKDESFSTAEQQQLHLM
                     ELIGQLKASPIAIETGAANEQHYEVPTAFFSMVMGKHMKYSCCYWDD"
     gene            565..993
                     /locus_tag="PLM90_13235"
     CDS             565..993
                     /locus_tag="PLM90_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90394.1"
                     /translation="MVKQFGLPNYVIWLFGGIAFVAVGTISVFHWTYGLATFILTTGV
                     AFAGYWFFFRPAFVALDIARKGEPAMAIILQVWETGRTVNDNPRIKLLLEIRHSTMVP
                     YEVEVRQNIAKDDMRLYQPGRTLQVKVDSRNPRKVAILAA"
     gene            1043..1453
                     /locus_tag="PLM90_13240"
     CDS             1043..1453
                     /locus_tag="PLM90_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMZ90395.1"
                     /translation="MVYLTRRETFNAAHRVYNNAWSEEKNKEMFGKCANKNWHGHNYQ
                     LFVTIKGKPDPDTGFIMDAKKLSDVIKREIVERFDHMNLNMDTPYFTDQQPSTENFCV
                     VIWALIEPFITGGVLHCIRLQETENIYAEYYGEQ"
     gene            1527..2096
                     /gene="folE"
                     /locus_tag="PLM90_13245"
     CDS             1527..2096
                     /gene="folE"
                     /locus_tag="PLM90_13245"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HMZ90396.1"
                     /translation="METLDNLIHHYQGIITSLGEDAEREGLVKTPERIAKAMQFLTQG
                     YNQDAEAVIRSAMFNEECSEMVIVKDIELYSMCEHHMLPFFGRAHVAYIPNGKIVGLS
                     KIARVVDIFSRRLQVQERLTHQILEALQNTLNPHGVAVVIEASHLCMMMRGVQKQNSV
                     TTTSAFIGQFQEVQTRNEFINLIAARLSK"
     gene            2159..2644
                     /locus_tag="PLM90_13250"
     CDS             2159..2644
                     /locus_tag="PLM90_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90397.1"
                     /translation="MAILHFVVTFTAKLNAMPNLRSTILMLFICSGLIAQAQPKQVKL
                     KGHKCPSIAISDPGEFYSTYNFSDNDLHALDSAGLKSTVIENIIAHHTETSWPGKLAS
                     LDERIANPDILKSYVVYKVAEINNMVVVLVPAKYNSDKPEGWALTEDVYFILSKAGVN
                     L"
     gene            2657..3532
                     /gene="fabD"
                     /locus_tag="PLM90_13255"
     CDS             2657..3532
                     /gene="fabD"
                     /locus_tag="PLM90_13255"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HMZ90398.1"
                     /translation="MKKAYVFPGQGSQFPGMGKDLYENNEAARALFERANEILGFRIT
                     DIMFNGTEEELKQTKVTQPAIFLHSVILASTLSDFNPDMVAGHSLGEFSALVANGALS
                     FEDALRLVSARAQAMQKACELQASTMAAVLGLADEIVEKICAETPGIVVAANYNCPGQ
                     LVISGEVEAVNKACEALKAAGAKRALLLPVGGAFHSPLMEPARIELSAAIEATPFNTP
                     RCPVYQNVTASAIIDPAEIKNNLLIQLTAPVKWTQSVTQMTADGATHYIEVGPGKVLQ
                     GLVKKISPTAEVASA"
     gene            complement(3608..4813)
                     /locus_tag="PLM90_13260"
     CDS             complement(3608..4813)
                     /locus_tag="PLM90_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ90399.1"
                     /translation="MAKKKQAKGFGSICVQPMATERKILSHNEPIFATTAYGFESLDH
                     ANMLFDDPSKGYVYSRWGNPTIEMAEKKIALLETSGSDIDATAQLFSSGMAAIAAAMT
                     SCATSGDTILTQHQLYGTTDELMQKQLAAWNINTMRMDLNDFDAVEKALKKNKKITLV
                     YIETPSNPMMSVTDIQTVAEIAHRYGRMVAVDNTFASPYCQKPLLLGADLVIHSATKY
                     LNGHGNGLGGVVVSRDRNFMRERIWMKVKLLGANSNAFDSWLLLQGMKTLEIRMQRHC
                     DNAAVVAAFLSDHHAVKKVHYCGHKDHPQRNIINRQMSDYSGMLSFELKGGLKAGKKL
                     MQSVKLCNVITTLGTLDTLIQHPASMTHVNVPKERRLAAGISDGLVRMSVGIENAADI
                     LNDLEQGMK"
BASE COUNT         1438 a         1030 c         1008 g         1406 t
ORIGIN      
        1 ttatcatccc aatagcagca ggaatatttc atatgctttc ccatcaccat actgaaaaaa
       61 gcagttggca cttcataatg ctgttcgttg gcagctccgg tttcgatggc gatgggcgag
      121 gccttcaatt ggccaatcaa ttccatcaga tgcaattgtt gttgttcagc ggtagaaaat
      181 gattcatcct tcagtcgccg gcgaagcaag gaacgaatcc gtgcacgaat caagaaatca
      241 ggaatcctgt cgcgttcaag taaagtattg atcacagttt tgggtttctt atgtgttaag
      301 aatgattcaa atatacggga acaagaagtt aacagatgtt tctttcccgg tgattgaaaa
      361 cagaaaagaa ctactatggt tcagcaaatt cggctattca aagggatttc gcgcaggaat
      421 tggtatataa attgcatgtt aaaacataca aacctccggc aattatgaac gccaatgtgc
      481 atttcgtggt tgtagaggtg aacatttctt gacattttta cgtatataat accataacct
      541 tttaacccaa tatcctcagc cattatggta aaacaattcg gcttacccaa ttatgtgatc
      601 tggctcttcg gcggcattgc ctttgttgcc gttggcacta tttcagtttt tcattggacc
      661 tatgggcttg caacatttat tctgacaacg ggagttgcct tcgccggata ttggttcttt
      721 ttccgtcccg cctttgtggc attggacatt gcacgcaagg gagaacctgc catggctatt
      781 attctgcaag tatgggaaac aggtcgcacc gttaacgaca atccgcgaat caaactttta
      841 ttggagatac gtcacagcac tatggtgcct tatgaagttg aagtgcgcca aaatattgct
      901 aaggacgaca tgcgccttta tcagcccggt cgcacgctac aagtgaaggt tgacagtcgc
      961 aacccgcgaa aagtggctat tcttgctgcc tgaggccggt caaattcact gattttattg
     1021 tatttacccg tatctttgcg ccatggttta ccttacacgt agagaaacct ttaatgccgc
     1081 gcaccgtgtg tacaacaatg catggagtga ggagaagaac aaagagatgt tcggcaaatg
     1141 cgcgaataaa aactggcatg gacataacta tcagttattt gttaccataa agggtaaacc
     1201 agatcctgac accggcttta ttatggatgc aaagaagctc agcgatgtta tcaaaagaga
     1261 gatcgtagag cgtttcgacc atatgaacct gaatatggat actccctatt tcacggatca
     1321 gcaacccagc accgagaact tttgtgtggt gatatgggcg cttattgaac ctttcatcac
     1381 aggtggtgtt ttacactgca tcagattaca ggaaactgaa aatatctatg cagaatacta
     1441 cggtgaacaa taacaagcgc atctcagatg ccgacgatga gttcaacatt tcagacagac
     1501 actataccaa aatcgattct tacggcatgg aaacgctcga caatctaatc catcattacc
     1561 aaggtattat cacttcatta ggtgaagacg ccgaacgcga gggactggtg aaaacccctg
     1621 aacgcattgc aaaggccatg caatttctta cacagggtta caatcaggat gcagaagcgg
     1681 tgattagaag tgccatgttt aacgaagagt gttctgaaat ggtaatcgta aaagatatcg
     1741 agctgtattc catgtgcgaa caccatatgc tgccattttt cggtcgtgct catgtggcat
     1801 atattccaaa tggaaaaatt gtaggactca gtaaaattgc tcgcgtagta gatatttttt
     1861 cccgtcgtct gcaggttcag gaacgtctta cacatcaaat tctggaagca ctgcaaaata
     1921 ccctcaatcc acatggtgta gctgttgtta ttgaagccag ccatttgtgt atgatgatgc
     1981 gtggcgtgca aaagcaaaat tctgtaacaa ccacttcagc atttataggc caatttcagg
     2041 aagtccaaac ccgcaacgaa ttcatcaatt tgatagctgc aaggttaagt aaataaggaa
     2101 ttcacggcgt ttcccattga tataggtctt tcttgtttct ttaccatagg tcccaactat
     2161 ggcaatcctg cattttgttg ttacttttac tgcgaaattg aatgctatgc ctaatctccg
     2221 ttcaacaata ctgatgctgt ttatctgcag cggtttaatt gcccaagcac agccaaaaca
     2281 ggttaaactg aaaggtcata aatgtccatc tatagccata agcgaccccg gtgaatttta
     2341 tagcacatac aatttctctg ataatgattt gcatgcgtta gattcggcag gattaaaaag
     2401 tacggtgatc gaaaatatta ttgcccatca taccgaaaca agctggcccg gaaaattagc
     2461 aagcctcgat gagcgtattg caaatccgga catactgaaa tcatatgtag tgtataaggt
     2521 tgcagaaata aataatatgg ttgtggtact cgtccccgct aaatataata gcgacaaacc
     2581 ggaaggctgg gcattgactg aagatgtata ttttattttg agtaaagcag gtgtaaatct
     2641 ctaatcaaca attgatatga aaaaagcata cgtatttccc ggacaaggtt ctcaatttcc
     2701 gggcatgggg aaagatcttt atgaaaacaa tgaagctgcc cgtgcattat tcgagcgcgc
     2761 taatgagatt ctcgggttcc gaataacaga tatcatgttc aatggaaccg aagaagaact
     2821 caaacaaaca aaggtaactc aacctgcaat attccttcac tctgtgattt tggcatccac
     2881 attatctgat tttaatcctg atatggttgc cgggcattca cttggtgaat tcagcgcatt
     2941 agttgccaat ggtgctttat catttgaaga tgccttgcga ttagtatcgg ctcgcgcaca
     3001 ggccatgcaa aaagcttgcg aattacaagc atcaaccatg gcagcagtat tgggacttgc
     3061 agatgaaatt gttgagaaaa tatgtgcaga aactccggga attgttgttg cagcgaatta
     3121 taactgtccc ggacaattag tgatttcagg agaagtagaa gcagtaaata aagcttgtga
     3181 agcattaaaa gctgccggag cgaaacgcgc attattgtta cctgtaggtg gagctttcca
     3241 ttcacctctt atggaacctg ctcgaattga attgagtgct gctattgagg ctacaccatt
     3301 taatacacct cgctgtcctg tatatcaaaa tgttacagca agcgcaatta ttgaccctgc
     3361 agaaataaaa aataatttgc tgattcaatt aactgctcct gttaaatgga cacagagtgt
     3421 tacacaaatg actgcagatg gcgctacaca ttatatcgag gttggtccag gaaaagtatt
     3481 gcaaggtctt gtgaagaaaa tcagccctac cgcagaagtt gccagcgcct aattgatgca
     3541 aatgatgctg cttccggcaa taaaaaatca gactgttgtt tttgataaca gtccaaatcc
     3601 cattgcatca tttcatccct tgttccaggt cattgagaat gtctgccgcg ttttcaattc
     3661 ccacactcat tctgacaaga ccatcagaaa tccctgccgc cagtcttctt tctttgggaa
     3721 cattgacgtg cgtcatactt gcaggatgct gaatcaatgt atccagtgtg ccgagtgttg
     3781 ttatcacgtt acataatttc accgattgca taagtttttt tccggccttt aatcctcctt
     3841 tcaattcgaa actcaacatc ccgctgtaat cagacatctg cctattgata atattgcgct
     3901 gaggatgatc tttatgtccg caataatgca cctttttcac tgcatggtga tccgataaaa
     3961 aagcggctac cacagcggca ttatcgcaat gtcgttgcat gcggatttcc aaggttttca
     4021 ttccttgcaa caataaccat gaatcaaatg cattactgtt tgcgccaagc aatttcactt
     4081 tcatccaaat gcgctcccgc ataaaatttc tatccctact tactaccaca ccaccaagtc
     4141 cattaccatg accattgaga tactttgttg ctgaatgaat tacaagatca gcgccgagta
     4201 ataaaggctt ttggcaatac ggagaagcaa atgtattatc aacagccacc atacgaccat
     4261 aacgatgtgc aatttcagca acagtttgaa tatctgttac cgacatcatc gggttggagg
     4321 gtgtttcaat ataaacaagt gtgatttttt tatttttctt caaggctttt tcaacagcat
     4381 caaaatcatt caaatccatg cgcatggtat tgatattcca ggctgcaagt tgcttttgca
     4441 tgagttcatc ggtagtacca tataactgat gttgtgttaa tatagtatct cctgaagttg
     4501 cgcaggaagt cattgcagca gcaattgcag ccatgccgct tgaaaacaat tgagcggtag
     4561 catcaatatc gctgcctgat gtttccagca aggcaatttt tttttctgcc atttctatgg
     4621 tcggatttcc ccatctggaa tacacatagc cttttgatgg gtcatcgaaa agcatattgg
     4681 catgatcaag tgattcaaat ccataggcgg ttgtagcgaa tataggctcg ttatgcgaga
     4741 gaattttcct ctcagtagcc attggctgca cacaaatgga gccgaaacct tttgcctgtt
     4801 ttttctttgc catacggatt gaaaatatgc cgttaaaatt aggtcattat ttactgacga
     4861 aacataaaaa tatacacatt ac
//
LOCUS       DAOQOU010000211         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10835_length_4868_cov_6.021816, whole genome shotgun sequence.
ACCESSION   DAOQOU010000211 DAOQOU010000000
VERSION     DAOQOU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10835_length_4868_cov_6.021816"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PLM90_13265"
     CDS             complement(<1..1260)
                     /locus_tag="PLM90_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ90400.1"
                     /translation="MYIQQLYTNCLAQAAYYIESEGEAVIVDPLRDVQTYIDLAASRN
                     AKIRFSLQTHFHADFVSGHLELARKTGAVIVYGPGAQPDYAAAVLKDYDVLDIGKVKI
                     MILHTPGHTIESTCFLLFDEENNVHSIFSGDTLFIGDVGRPDLLSGNLDAETLGGMLF
                     DSLQNKIKVLPDDCIVYPGHGAGSACGKNLGKETTSTIGIQKATNYALQFADKQAFID
                     AVIAEQPAAPAYFFKDARINIHGYESLDKVMDAAMKGIDAATVQLETAEGALILDTRP
                     AAVFAKGFIPGSLNIGLNGEFAVWAGTLVDISTPLILISEEGKEKEAVTRLARIGYEN
                     ILGYLEGGFASWKSEKLPTSTIKNVSVDDFANLMDSGKYALLDVRKPGEVEKNKVFEA
                     HHISLNKLAAEMDTLDPKNKYIVYCGGG"
     gene            complement(1378..2508)
                     /gene="hppD"
                     /locus_tag="PLM90_13270"
     CDS             complement(1378..2508)
                     /gene="hppD"
                     /locus_tag="PLM90_13270"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HMZ90401.1"
                     /translation="MDTIVLDQKKTNVQEDFLPLNGTDFIELYVGNAKQSAHYYKTAF
                     GFQSLAYSGLETGNRECASYVLRQGKITLVLTTPYHSDSPVAEHVRRHGDGVKVIAFW
                     VDDAEATFEKTIARGAEPFMYPEARTDDTGEVKIAGIKTYGEVIHLFVERSNYNGVFM
                     PGFVKWESEYNPTPIGLEYVDHCVGNVGLGRMNVWAKFYEEVLGFKNLITFDDKDIST
                     EYTALMSKVMVNGNMRIKFPINEPAKGKKVSQVQEYLDFYEGEGCQHIAVATDDIIHT
                     ITEFRKRGVEFLYVPGTYYDTVLDRVGKIKEDLEVLKSLGIMVDRDEEGYLLQIFTKP
                     VEDRPTLFFEIIQRVGAKSFGKGNFQALFESIEEEQRKRGTL"
     gene            complement(2564..3727)
                     /locus_tag="PLM90_13275"
     CDS             complement(2564..3727)
                     /locus_tag="PLM90_13275"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HMZ90402.1"
                     /translation="MYHQLGKIPNKRHVVYRNSEGQMYNEELVGTQGFSSLSTLAYHI
                     YPPTRVIGTGTPFDMRPRIAIENNMRMLSFSGFDIPAEDDYLQSRKVLFVNSDLHIGL
                     AAPTQSPEYFFKNADADELIFIHKGSGTLSTMYGEVEFEQHDYLVIPRGTIYKIDFDT
                     DDNRFLFIESFSPIYTPRRYRNEFGQLLEHSPFCERDFKKPANLITHDEEGEFEILIK
                     KRGQVFPYTYANHPFDVVGWDGYLYPYAFNIHNFEPITGRIHMPPPIHQNFEGNNFVV
                     CSFVPRLYDYHPQAIPAPYHHSNIDSDEILYYVEGEFMSRNNIKQGQLTLHPAGLPHG
                     PHPGAIERSIGKKETHELAVMIDPFKPVSITETAVELEVEDYYLSWISKQLTH"
     gene            4074..4460
                     /locus_tag="PLM90_13280"
     CDS             4074..4460
                     /locus_tag="PLM90_13280"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HMZ90403.1"
                     /translation="MSFSAKRARELRRRQTQAEAIMWKELRKYRKIKFPFRRQHPVTL
                     VEISNSTILYVLDFYCMPIKLAIEIDGPVHLQSEVIHYDKHRQSLLEEMGIMVVRFTN
                     DEVYHNLEYVIKTIEDCIATRIKGLS"
BASE COUNT         1419 a         1024 c          958 g         1467 t
ORIGIN      
        1 cccgccgcca caataaacga tatatttatt tttaggatcc aaagtatcca tttcggccgc
       61 aagtttattc aaagaaatat ggtgtgcttc aaacacttta tttttttcaa cttcaccggg
      121 tttgcgcaca tccagcaatg catattttcc gctatccatc aaattggcga aatcatctac
      181 acttacattt tttatagttg aggttggcaa tttttcagat ttccatgatg cgaatcctcc
      241 ttccagatac cccagaatat tttcatagcc aatgcgcgcc aatctggtta ctgcttcttt
      301 ttcttttcct tcttcagaaa ttaaaatcag tggtgtagaa atatccacca gtgttcctgc
      361 ccatactgca aactcgccat tgaggccaat attcagtgaa cccggaataa atccttttgc
      421 aaaaacagca gcaggtcttg tatcaagaat caaagctcct tcagcagttt ctaactgtac
      481 agttgccgca tcaattcctt tcattgcagc atccattact ttatccaaag attcatatcc
      541 atgaatattt attctggcat ctttgaagaa atatgcaggt gctgcaggtt gttcagcaat
      601 aacggcatca ataaatgctt gtttgtcggc aaattgcaat gcatagtttg ttgctttttg
      661 tatgccgatg gtactggtag tttctttacc taaattcttg ccgcaggcac ttcccgcacc
      721 atgaccggga tatacaatac aatcgtcggg cagtactttg attttattct gcaaagaatc
      781 gaacaacatg ccacctaaag tttcagcatc gagattaccg ctaagcaggt caggacgacc
      841 aacatcgcca ataaataaag tgtcgccgct gaaaatgcta tggacattat tctcctcatc
      901 aaacaacaaa aaacatgttg attcgatggt gtgcccgggc gtatgcagga tcataatttt
      961 cactttcccg atatccagca catcataatc tttcaacact gctgcagcat aatcaggttg
     1021 tgcacccgga ccatacacaa ttacagctcc tgtttttcgt gcaagttcca aatgacctga
     1081 aacaaaatcg gcatggaaat gtgtttgcag actgaagcga atttttgcat ttcttgatgc
     1141 agcaagatcg atataagtct gcacgtcgcg caatgggtca acaattacag cttcgccttc
     1201 gctttctata taatacgctg cctgtgcaag gcagtttgta taaagttgtt gaatgtacat
     1261 gtgaaagaat tttaatgatg atcgaatatt tgcatctgca atcttagatg actttatcaa
     1321 aaatgagata cgtcctaaaa ggcatcaggc agccactaag gactgcctga tgttgtatca
     1381 caatgtacct cgttttcttt gttcttcctc tatgctttca aataaagcct ggaagtttcc
     1441 tttaccaaat gattttgctc caacgcgttg aatgatttca aaaaataatg taggtcgatc
     1501 ttcaaccggt tttgtgaaaa tctgcagaag atatccttct tcatctcggt cgaccataat
     1561 tcccaagctc tttaaaacct ccaggtcttc cttgatcttt cctacgcggt ctaaaaccgt
     1621 atcataataa gtaccgggca catacaagaa ttcaacaccg cgcttgcgaa attctgttat
     1681 agtatgaatg atatcatcag ttgctactgc aatgtgctga caaccttctc cctcataaaa
     1741 atcgagatat tcctggacct gactaacctt ttttcctttt gcaggttcgt tgatagggaa
     1801 ttttatgcgc atattgccat tcaccatcac cttgctcatt aaggcagtgt attcggtgga
     1861 aatatcttta tcatcgaagg tgatcaggtt tttaaaaccc agtacttctt cataaaattt
     1921 agcccataca ttcatgcgac cgaggccaac attacctacg caatggtcta catattccaa
     1981 accgataggc gttggattat attcggactc ccatttcacg aatccgggca tgaatacgcc
     2041 attataatta ctgcgctcaa caaataagtg aattacttcc ccatatgttt tgatgcccgc
     2101 aatttttact tcaccggtgt catcagtacg cgcttcagga tacatgaatg gttccgctcc
     2161 gcgtgcaata gttttttcaa aagttgcttc tgcatcatcc acccaaaaag cgattacttt
     2221 cacgccatcg ccatgtcgac gaacatgctc cgctacaggg ctgtcgctat gatatggcgt
     2281 tgtaagcaca agagtgattt taccttgacg aagcacatag gatgcacatt cgcggttgcc
     2341 ggtttcaagt ccggaataag caagactctg aaacccaaaa gctgttttgt agtagtgagc
     2401 cgattgtttg gcattaccta catacaattc gataaaatcg gttccattca atggcagaaa
     2461 atcttcctgc acatttgttt ttttctgatc taatacgata gtatccatag ttgtttattt
     2521 caggtttatc ccttacagaa aataatttac tgctcaggaa tttttagtgg gttagttgtt
     2581 ttgaaatcca tgataaatag taatcttcca cttcaagctc tacagcagtt tctgtaattg
     2641 aaacaggctt gaaaggatca atcatgacag cgagttcatg tgtttctttt ttaccgatac
     2701 ttctttcgat tgctcccgga tgcgggccgt gtggcaatcc ggcagggtgc agtgttaact
     2761 gtccctgttt gatattattg cggctcataa attcaccttc cacataatat aatatttcat
     2821 cgctgtcgat gttgctatga tggtaaggtg caggaattgc ctgtggatga taatcataca
     2881 agcgaggaac aaatgagcat accacgaaat tattgccttc aaaattctga tgtattggcg
     2941 gaggcatgtg aatgcgacct gttattggct caaaattgtg aatgttaaac gcatatggat
     3001 ataaatatcc atcccaacct acaacatcga atgggtgatt ggcataagta tatggaaata
     3061 cttgtccacg ttttttaatc agtatttcaa attcgccttc ttcatcatgt gtgatgaggt
     3121 tagcaggttt tttgaaatcg cgttcgcaga atggtgaatg ttcaagcaac tgaccaaatt
     3181 cattgcggta gcgacgaggt gtatatattg gactgaacga ctcgataaac aggaatctgt
     3241 tatcatctgt atcgaagtct attttatata tggtaccgcg agggataacc aaataatcat
     3301 gttgttcgaa ttccacttcg ccatacatgg tactgagcgt accggagcct ttgtgaatga
     3361 atatcagctc atcggcatct gcatttttaa agaaatattc aggcgattgc gttggagctg
     3421 caagtccgat gtggagatcg ctgtttacga acaacacttt tctgctttgc agataatcat
     3481 cctcggcagg aatatcgaaa ccgctgaaac ttaacatgcg catattattc tcaatagcga
     3541 tacgaggacg catatcaaat ggtgttccgg tgccaatcac ccttgttgga ggatagatat
     3601 gataggcaag tgtagaaaga ctgctgaatc cttgtgtgcc gaccaattct tcattgtaca
     3661 tctgaccttc agaattgcgg taaacaacat gtcgcttatt cgggattttg cccagttgat
     3721 ggtacattgg cataggtatg actatttata gtacaaattt ccctgtatac ccacccttgc
     3781 gaggtgactt atgtcaatgt tggcggtgat gtttgaacga gaaacgctac gggtgaaagt
     3841 caccgattgg tgtaaaagtg ttatgtgctt ggttcgcgct aagacgctaa gttgcgaaaa
     3901 cgcaaagcat gcattgggcc tgacgatgtc tgatatcaat actaataata tccgggcggt
     3961 taaccgatga tcaatccaca ggctgattta agtatagaaa gtctaataag cggttagtgt
     4021 ggatattttc ggtcagaagt accaacatta gtaataaata tacaccaaaa aaaatgagtt
     4081 ttagcgcaaa aagagcccga gaattaagaa gaagacaaac tcaggcagag gctattatgt
     4141 ggaaggaatt aagaaaatat agaaaaatca aattcccatt cagacgtcag caccctgtca
     4201 ccctagtaga aatatcaaac tcaactattt tgtatgtgct tgatttctat tgtatgccaa
     4261 ttaaattagc tattgagatt gatgggcccg tccacttaca aagtgaagtt attcattacg
     4321 acaagcatag acaatcctta cttgaggaaa tgggaataat ggtagttagg tttacaaatg
     4381 atgaagtcta tcacaatcta gaatatgtaa taaagacaat tgaagattgt attgcaacaa
     4441 gaattaaggg actatcatag tatcagggaa tgcgagggag tagtgggaca gttaattatc
     4501 attttctgat ggaggattgt ggtagtagta gagaattacc gccgccctca ccccgaccct
     4561 ctcccagggg gagagggagt aacccttgat tctaagctaa atttatcatg gatacgacaa
     4621 ataccccctc tccccctggg agaggggcgg ggtgaggggc gacaagttac ctagcaaggt
     4681 aaaacataat ctgtttgaat atccctaatg catcacaaaa ctcacacaac acaatatctc
     4741 tcacacctcc gcaaaccgtg ctgtaaaatg cctcagaaaa gcctgctcat agatgatttt
     4801 caggcctttt gctttgagtt tatttttttc tacttcgcga aatacttcga tcacataatc
     4861 aatatgac
//
LOCUS       DAOQOU010000212         4823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_10987_length_4823_cov_5.675965, whole genome shotgun sequence.
ACCESSION   DAOQOU010000212 DAOQOU010000000
VERSION     DAOQOU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10987_length_4823_cov_5.675965"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1278
                     /locus_tag="PLM90_13285"
     CDS             202..1278
                     /locus_tag="PLM90_13285"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ90404.1"
                     /translation="MSRLKLSPGAFFASGTGRVILHVVFWILYMNSPIFMMEVNTFSS
                     NGILLFEVMNLLFIPYYYLLAYVLIPKLFTLKRSPLFVVSWVGSYVLMAYCVQQVSIH
                     FLYTLTTEAEIATFKKVAETSPTYFPEFFRMIFVTMVPLSIMLVRRYARISAKQTELK
                     RMNADLELNFLKSQMNPHFLFNSLNNIYALALQQSEKTPGLILQLSDLMRYMLYECNA
                     AQVALDKELNFLRDYIGLEKVRHGEKVKIEFNIKGETSQKQIAPLLLLPFVENAFKHG
                     VNAQLGPAWVSVDFNAEDPEGGCTFVVRNNKPVKTEKTSSRAGGIGLENARKRLDLLY
                     PNKYLLHVDNHNEVYQVELKIMDL"
     gene            1275..1973
                     /locus_tag="PLM90_13290"
     CDS             1275..1973
                     /locus_tag="PLM90_13290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ90405.1"
                     /translation="MNHKLRCIVVDDEPLAQQVIVTYIGRLSHMELVATCDNAMEAMQ
                     ALQKDKIDVMFLDIQMPVITGVEFLRSLQHPPRVIFTTAYPDFALDGYDLNITDYLLK
                     PISFERFMKAVNKLSEAVNNEEHNTPIPDHFFVKEDGKLVRVNFADIDHIECMKDYAK
                     IFTKQRMIVTHHTMKKFEEVLPDQQFMRIHRSYIVSLSSINAIFGNVIETAKGRLPVG
                     ANYREMLMEKIKVD"
     gene            2018..2488
                     /locus_tag="PLM90_13295"
     CDS             2018..2488
                     /locus_tag="PLM90_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90406.1"
                     /translation="MKKILTLVLAFTFFSQAHAQINDHAIGIRFGFGGGISYQQKMSK
                     TNRLELNGSANFYNGYSAFRLTGVYQWVWDMNKGFSFYAGPALSTGTMSLGDNYNGKG
                     KNGFFVSGGGQVGLDYNFDGLPLQFSVDVMPLFPIINNVDDFYLDPALGVRFVF"
     gene            complement(2546..3088)
                     /locus_tag="PLM90_13300"
     CDS             complement(2546..3088)
                     /locus_tag="PLM90_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HMZ90407.1"
                     /translation="MQQYYPYFLAAHIIFVITWFAALFYIVRLYIYHTEALEKQEPER
                     SILAAQFTIMEQRLMAIIGTPSMILVVITGVSLLSIQSQFLKEPWLHTKLLFVIGVIV
                     YHLACLRIQKQLREGIAKNSSTKLRMFNEVATVLLVAIVFLVVLKDLVDMLYGLLGLI
                     ALSFSMLVAIRIYKRRREKR"
     gene            complement(3091..>4823)
                     /locus_tag="PLM90_13305"
     CDS             complement(3091..>4823)
                     /locus_tag="PLM90_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ90408.1"
                     /translation="FIQQEKGTFVVQSGTMKTQTRIFIIAIVLACAQINAFAQPCAFF
                     EDYTSDAGWTDYYYYYAVTGACLSDEQDGSLTFGGGKLNFNALDDANDTRIYFSLPTP
                     AYEDFWTVSGEFTPTAGGASGRTGSMIVALSNGSDNPISDTYSICEFSNTDAIALMWT
                     SDFDAAPDNIGFEIWANDNGTVTMSERLIAPYGDTYYFQWTRIAIEYMSLDVYLDATH
                     TDLFATIPCFYLPTTITDLNTMQSGNFPGGSYLRQLTATLDNVCLRNIDVTAASITGP
                     ETICLGSENDYTITTFAGADIDWTLPAGITYTGDAAPTIHVTDWPGAGTYTLMSVINY
                     NCFYDTAYYEVTVIDPGSGEVLEEDFCEGGSTTVDVTTTDATYLWDDGTTEGTHSYDT
                     EGTYWVTIYIAGCEFTDTIHITEYPNPSIDLGDDMDICGDVTVEGPSGFTSYEWNTGS
                     ASANLTTSIPGNYSLTVTDENGCTAFDDITLNDACADHVVMPTVFSPNNDGINDWIGP
                     NYSGDIKDYHLEIYNRYGQLIFETDEIAQGWNGKFENELQPIGTYVYVMQVKLNNKKR
                     EERGNITLVR"
BASE COUNT         1377 a          951 c         1035 g         1460 t
ORIGIN      
        1 aactgagctt ccatcttctc tgctgttgaa ctgcaatgtt aaacactttc acagaacgga
       61 ttggtatgtg gggcctttgg ttttacatat ttctgacaat aaacgcctga aaatcaacat
      121 ttactgcact tttcgtcgaa ggtggaggct gtttagtcga aattccggct ccgatagctc
      181 cattttcggc acttttgcat catgtcgcgc ctgaaattgt cgcccggagc cttttttgcc
      241 tccggaaccg gtcgtgtgat actgcacgtc gtgttctgga tactctatat gaattcaccc
      301 atctttatga tggaagttaa taccttctca agcaacggca tcctgttgtt tgaggtgatg
      361 aacctattat ttatccctta ttattacctg ctggcttatg tactgattcc taagctgttt
      421 actctaaagc gttcacctct ttttgttgtt tcctgggttg gttcttacgt attgatggca
      481 tactgcgttc agcaagtgtc tattcatttc ctatacacac tcaccactga ggcggaaata
      541 gcgactttta aaaaagtggc agaaacatca cccacctatt ttcccgaatt tttcaggatg
      601 atatttgtga ccatggtgcc gctgagtata atgttggtta ggagatatgc gcgcatctcg
      661 gcaaaacaaa cggaactcaa aagaatgaat gccgatctgg aattgaattt tctgaaatcg
      721 caaatgaatc cccatttttt atttaatagt ctgaataata tttatgcatt ggcattgcaa
      781 caatcagaaa aaacgcccgg actcattttg caacttagtg atctgatgcg ctatatgctt
      841 tacgaatgta atgctgcgca agtagctctt gataaggaat taaatttctt gagagattat
      901 atcggacttg aaaaagtgcg acatggagaa aaggttaaaa ttgaatttaa tatcaaggga
      961 gaaacttcgc aaaagcaaat agctccctta ttactgctgc ccttcgtgga aaatgcattt
     1021 aagcatggcg taaatgcgca actcgggccg gcatgggtgt ctgtcgattt taatgccgaa
     1081 gatccggaag gaggatgtac atttgtagtg cgcaacaata aaccggtaaa gacagaaaaa
     1141 acttcgtcgc gggcaggagg cataggtttg gagaatgcgc gtaaacgatt ggatttgttg
     1201 tatccgaata aatatttgct gcatgtagat aatcacaacg aagtgtatca ggttgaatta
     1261 aaaattatgg atttatgaat cacaaattga gatgtattgt tgttgatgat gaaccgctgg
     1321 ctcaacaagt gattgtaact tatatcggac gtctctcgca catggaattg gtggctactt
     1381 gcgataatgc catggaagcc atgcaagctt tacaaaaaga caagatagat gtaatgtttt
     1441 tagatattca aatgcccgtg attacaggcg ttgaattttt gcgcagtctg caacatccgc
     1501 cgcgcgtaat tttcactaca gcatatcccg attttgcttt ggatggttat gatttgaata
     1561 ttacggatta tctgcttaag ccaatttcat ttgaacgatt tatgaaggct gtcaataaat
     1621 taagtgaagc ggtaaacaac gaggaacata atacgccgat tccggatcat ttctttgtga
     1681 aagaagacgg gaaactggtg cgcgtgaatt ttgctgatat tgatcatatc gaatgcatga
     1741 aagattatgc caaaatcttt acaaagcaac ggatgattgt tacccatcat acgatgaaga
     1801 aatttgaaga agtgctgcct gaccaacagt ttatgcggat acatcgcagc tatatcgtaa
     1861 gcctttcgtc tattaacgcg atttttggga atgtcataga aaccgctaaa ggccgcttgc
     1921 cggtaggcgc caactacagg gagatgctta tggaaaaaat caaggtcgac taacagggga
     1981 ttgattggaa ttccgttact ttgtaaaaaa ctgcaatatg aagaaaatcc ttaccctagt
     2041 tctggcattt acttttttct cacaggcaca tgcgcaaatc aatgatcatg ctataggtat
     2101 ccgatttggc tttggtggtg ggatctcata ccagcaaaaa atgagcaaga cgaaccgact
     2161 cgaattgaac ggctccgcga atttttataa tggctactca gcctttcgat tgaccggtgt
     2221 gtaccagtgg gtttgggata tgaataaagg tttcagtttt tatgcagggc ctgctttgtc
     2281 gaccggcaca atgagccttg gtgataatta taatgggaaa ggaaaaaacg gattttttgt
     2341 cagtggcggc ggtcaagtag gattagatta caattttgac ggattaccgc ttcagttttc
     2401 agtggatgtg atgccgctgt tcccgattat taataatgta gatgattttt atctcgaccc
     2461 tgcattgggc gttcgctttg tgttctaagc gacgataata acattatgtg aggttgtcgg
     2521 cttgatgcac ggcaaccttt ttttattacc ttttttctct acggcgtttg taaatgcgta
     2581 tggcaacgag catgctgaag gaaagtgcta tcaaccctaa taatccatac aacatatcca
     2641 cgaggtcttt caatacaaca agaaaaacga tggctactaa taacactgta gctacttcat
     2701 tgaacatacg cagttttgta gagctatttt tagctattcc ttctcgcaat tgtttttgta
     2761 tgcgaagaca tgccagatga taaactataa cgccaatcac aaaaagcagt ttggtatgca
     2821 accatggttc tttcaagaat tgtgattgaa tgctgagcaa tgacacaccg gtaattacaa
     2881 caaggatcat ggatggcgtg ccgataatag ccattagtcg ttgctccata atggtaaact
     2941 gtgctgccaa aattgagcgt tccggctctt gcttttcgag cgcttcagta tgatagatat
     3001 ataatcttac aatataaaac agtgcagcga accatgtaat cacaaagatg atatgtgctg
     3061 ctaaaaaata tggataatat tgttgcatgg ttatcgtact agcgttatgt tgccccgttc
     3121 ttcacgtttt ttattattca acttaacttg catgacatat acataagttc cgataggttg
     3181 taattcattt tcaaattttc cattccaacc ttgagcaatt tcgtcggttt cgaaaataag
     3241 ttggccgtat cggttataaa tttctaaatg ataatctttt atatcacccg aataatttgg
     3301 gcctatccaa tcatttatgc catcattatt tggtgaaaaa acggttggca ttaccacatg
     3361 gtcggcgcag gcatcattta aagtaatgtc atcgaaggca gtgcatccat tttcatctgt
     3421 aacagtcagt gagtaattac cgggtatgga agttgtcaaa ttcgctgacg cactaccggt
     3481 attccattcg taactcgtaa atccgctggg accttcaact gttacatctc cacatatatc
     3541 catatcatca cccagatcaa ttgatggatt tggatattct gtgatgtgaa tggtatctgt
     3601 aaactcgcat cctgcgatat agatggttac ccaataggtt ccttcggtat cataagaatg
     3661 tgtgccttct gttgtgccat cgtcccataa ataggttgca tcagtagtag ttacatcaac
     3721 ggttgtactt cctccttcac aaaaatcttc ctccaacact tcgccgcttc ccggatcgat
     3781 tacagttact tcgtaatatg cggtatcgta aaaacaatta tagttgataa ctgacatcag
     3841 tgtgtatgtt ccggcaccgg gccaatcagt tacgtgaatg gttggtgctg catcgccggt
     3901 ataggtaatt cctgccggca aagtccaatc aatatctgct cctgcaaatg tggtgatggt
     3961 gtagtcattt tcactaccta aacaaattgt ttccggtcct gtaatacttg ctgcggtaac
     4021 atcgatattt cgcaaacaaa cattgtctaa agtagctgtt aactggcgta aatatgaacc
     4081 gccggggaag ttccccgatt gcatggtatt taggtctgtg atagttgtag gcaaataaaa
     4141 acagggaatg gttgcaaaca gatcggtatg tgtcgcatcc aggtaaacat ccaacgacat
     4201 gtattctatt gcaatgcgtg tccattgaaa atagtaggta tcgccataag gagcaataag
     4261 tcgttcactc atagtgactg ttccattatc attcgcccat atttcaaatc cgatattatc
     4321 aggtgcggca tcaaaatctg aggtccacat aagtgcaatg gcatctgtat ttgaaaattc
     4381 gcaaatcgaa taagtatctg aaatagggtt gtcagatcca tttgaaagcg caactatcat
     4441 agaacctgtg cgcccgcttg ctcctcctgc tgtaggggta aactctccgc tgactgtcca
     4501 gaaatcctca tatgccggag tcggaagtga aaagtaaata cgggtgtcgt ttgcatcatc
     4561 aagggcgttg aaattcagtt tgccacctcc aaaggtgagc gagccatcct gttcatcgct
     4621 aaggcatgct ccggtaacag catagtaata gtagtagtcg gtccaacctg cgtcagaggt
     4681 gtaatcttca aagaaggcgc aaggctgagc gaaagcatta atttgcgcac atgccaatac
     4741 tatggcaata atgaagattc gggtttgggt tttcattgtg cctgattgaa caacaaaggt
     4801 acccttttcc tgctgaatga aat
//
LOCUS       DAOQOU010000213         4747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_11284_length_4747_cov_4.949062, whole genome shotgun sequence.
ACCESSION   DAOQOU010000213 DAOQOU010000000
VERSION     DAOQOU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11284_length_4747_cov_4.949062"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLM90_13310"
     CDS             complement(<1..893)
                     /locus_tag="PLM90_13310"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HMZ90409.1"
                     /translation="MRDYGFDDQAFSSMVEDEVEFLPILSLEEDEDNTKIEYPETIVV
                     MPLRNTVLFPGIVLPITVGREKSIKAIQNAFRSNKLIGVVAQKDGNIEDPETNQLYTT
                     GVIAKVIKQLKMPDGSTTVIIQGKKRFVINEFTTKEPYLQAKISLLEDKPHPDQHEFE
                     AQISAIRDLANSIVKVSPNIPQEASVVLRNINSPIYLLHFVSSNLQIEVADKQKILEA
                     DNLQSRAQLVLNHLTSELQLLELKNKIQNKVRNDMDKQQREYFLNQQLRTIQEELGGS
                     GQDEEIKRYRQRAEDKKWAKHV"
     gene            1040..1663
                     /locus_tag="PLM90_13315"
     CDS             1040..1663
                     /locus_tag="PLM90_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90410.1"
                     /translation="MTSLLLYKQLFVYLIPFNVLIGEYNLGMQYRIGEEWAVDLNLGY
                     VDDTEGALTAELYQDILQSGVFYYHGPLVKVSALSLVPRGPNPLKTDYNQLEFACRFL
                     RYDNLDFEDENEPGKLFNISEQMKAVSLSWKAGYIIVPRNTFEMNGFVGFGIQTRFKN
                     TIVNSYGTNYQSNVFILNDVSSSIQFVPLFHAGIKIGLKTPLKESSQ"
     gene            1675..3891
                     /locus_tag="PLM90_13320"
     CDS             1675..3891
                     /locus_tag="PLM90_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ90411.1"
                     /translation="MRIKSIILIYTLLLTNILAAQNFDFNNFQTLRSSGTVPDDFTEK
                     SSVKFQRDKSEIESDSKREQKDMENFLLASNYEIEDMLHSGRIVFGDPITNYLNDIKS
                     EILKGDPQLAKSIRIYTLLSNDVNAFTFDNGIVVVTTGLVSQVQNEAQLAYILCHEFV
                     HFTEKHAITSYVENRRVDRGAGVYASLGRDEEDLVKFSYAKDQESEADTKGLDLFKTT
                     NYSYDAISGVFDVLLYSYLPINEIVFPKDYFNEGMYKLPDNAFIDTINQITAVEDYDD
                     AKSTHPNIKKRKSDVLGDIPATSDASRKLNILGEARFNEIQQIARFQGCELYLIDVHY
                     EDALYQAFVLQQDFPDNPYLKRVIAQALYGWTIYKNAGTTPTGHIYYKKVEGESQQVF
                     HLFSKLNAKEFNILALKYAWDVHLAEPDNKQMTKICQHLGKQLRNEHDIDYDNFFTET
                     EVKKFIDAMNAKNDSTAVQPVDSVGKTNDTKTSKYDKIKKEKNTIDEKAPYWRFAFAS
                     VDHSAEFKALFEEEEELVATTSKKKKKSEFNLGLKELVIVDPVYISIDERSNNPVQYE
                     AAEDAKLNLKDKVKASADELNLKVEYLDHTNIDDRDVETFNEMAVLSRWIEEKLTHID
                     EDIFIETSTNDELQEIAKSLDIDHFAWMGIVSFTESEEYVGAKIFLCLYLPLAPFLIY
                     DLVTPDRNTFYFALVANAETGKLEMQYFNSSTLNDSNAAQSSNIYYILQQIKGRKK"
     gene            3888..4721
                     /locus_tag="PLM90_13325"
     CDS             3888..4721
                     /locus_tag="PLM90_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90412.1"
                     /translation="MKRIILALCSLAISASAIAQVPGYMGKRLQIYASVEPTFDFRYI
                     TDGLDYYLQQPEVTIPTEYGNYGIGVTPGVGIEYTLNNTFVMGADVKWKQAKAQLHMY
                     YNTASNPDIPYGYLGEGKISGMLTGFYFKIYSHRKHGYIAPIGRYHQFEILTGKFSVQ
                     ESGYSSTDFYAVDYQEYYSNLSLQASELTPVASLSQLNFDPHRISYLRYVFGGETVLF
                     DKVPADIGIQFAMPLSFVQYSDYDNGIDKGGYDYAYTVRKSMNGAMAVGIVMRFGIFA
                     I"
BASE COUNT         1457 a          922 c          959 g         1409 t
ORIGIN      
        1 acatgctttg cccatttttt atcttccgcg cgctggcgat agcgtttaat ttcttcatcc
       61 tgacctgaac cgccaagttc ttcctgtatg gtacgcaact gctgattgag gaaatattcg
      121 cgttgctgct tatccatatc attgcgcacc ttattctgaa tcttgttttt taattccaga
      181 agctgcagct cactcgtcaa atgatttaat accaattgtg cacgcgattg caaattatcg
      241 gcttcgagaa ttttttgctt gtcggcaact tcaatctgaa gatttgaaga aacaaaatgc
      301 aagagatata tcggactgtt aatattgcgc aatactacac tggcctcctg cggaatattt
      361 ggcgatactt tcacgatact gtttgccaga tcgcgaatag cactgatttg tgcttcaaat
      421 tcatgttgat cgggatgcgg tttgtcttcc agcaaactga tcttggcctg caggtaaggt
      481 tctttggtag tgaattcgtt aatgacaaat cgcttttttc cctgaatgat aacagttgtg
      541 ctgccatccg gcattttcaa ttgcttgatc accttcgcga taacacccgt agtatacaat
      601 tgattagtct ccgggtcctc gatattgcca tccttttgtg caactacacc gattaactta
      661 ttgctgcgaa aagcattttg tatggcctta atagatttct ctctgccaac agttataggt
      721 aacactattc ctggaaacaa aacggtattg cgcaagggca tcaccactat cgtttcaggg
      781 tattctattt tcgtattgtc ttcgtcctct tcaagcgaca gtatgggtag aaattctact
      841 tcatcttcca ccatcgacga aaaggcctga tcatcaaatc cgtaatctct catatgcgat
      901 cttttgtgaa ttcaaggcag tctccgacca ccccgcaatt tgtgttcaat tgttgtgcct
      961 gtcccattcc gactgccaga atacgcgaaa tctgccaaaa gccgtacatt tttctgccaa
     1021 tttggtagat ttgtccatta tgacctccct gctcctctat aagcagctat ttgtctacct
     1081 gatacccttc aatgtcttga ttggcgagta taacctgggt atgcaatacc gtattggtga
     1141 agagtgggcc gtagacctca atcttggcta tgttgatgat acagaaggtg cgcttaccgc
     1201 agaactctat caggatatcc ttcaaagtgg cgtgttttat taccatggac cactcgtaaa
     1261 agtttctgca ttaagtcttg ttccaagagg gccaaacccg cttaaaaccg attataatca
     1321 attggaattt gcctgccgtt ttctaaggta cgacaacctc gattttgagg atgaaaatga
     1381 accgggcaaa ttgttcaata tcagtgagca gatgaaagca gtcagtttgt catggaaggc
     1441 aggatatatt attgtgccga gaaatacgtt tgaaatgaat ggttttgtag gatttggaat
     1501 tcaaacgcgt tttaaaaata cgattgtaaa tagttatggt acaaattatc agtcaaatgt
     1561 atttattttg aacgatgtat ccagttccat tcaattcgtc cctttatttc atgccggcat
     1621 taaaattggc ctgaaaacac cattaaaaga atcatctcaa taaagtaaac atacatgcgc
     1681 ataaaaagca ttatcctcat ctacacactc ttgctcacca atattctggc agcacagaat
     1741 ttcgacttca ataatttcca aacactgcga tcctccggaa ctgttcctga tgattttact
     1801 gaaaagtcat ccgtgaagtt tcagcgcgat aaatcagaaa tagaatccga tagcaaacgc
     1861 gagcaaaaag atatggagaa tttcctgctc gcctccaact acgaaataga agatatgctg
     1921 cacagcggac gtatcgtttt tggagacccg attaccaatt atctgaatga cattaaatca
     1981 gaaatattaa aaggtgatcc gcagttggcg aaatctatac gtatttatac cttgctctca
     2041 aacgatgtaa atgctttcac ttttgataat ggaattgtag tagttacaac cggactggtt
     2101 tctcaggtac aaaatgaagc acaattggca tatattcttt gccacgaatt tgtgcatttt
     2161 accgaaaaac atgccattac ttcctacgtt gaaaacagaa gggtggatcg cggcgccggt
     2221 gtttatgcaa gtcttggtcg cgatgaagaa gatcttgtga aattctctta cgccaaagat
     2281 caggagtctg aagctgatac caaaggctta gaccttttca aaacaacgaa ctatagttat
     2341 gatgctattt caggtgtatt tgatgtgcta ctgtatagct atctgcctat taatgaaatt
     2401 gtatttccaa aagattattt taatgaagga atgtacaaac tccctgataa tgcattcatt
     2461 gataccataa atcaaatcac tgcagttgaa gattacgatg atgctaaaag cacgcatcct
     2521 aatatcaaga aacgcaaatc agatgtgttg ggcgatattc ctgcaacaag tgatgcatcg
     2581 cggaaattaa atatccttgg agaggcaaga tttaatgaaa tacaacaaat tgcccgcttc
     2641 cagggatgtg aattatatct gatagatgtg cattatgaag atgcattata tcaggcattt
     2701 gtattgcaac aagattttcc ggataatccc tatctcaaaa gggtaattgc tcaggcattg
     2761 tatggttgga caatttacaa gaacgcagga acaacaccta caggtcatat ttattataaa
     2821 aaggtagaag gcgagtcgca acaagtattt cacttattca gtaaattaaa tgcaaaagaa
     2881 tttaatattc tggcattgaa atacgcatgg gatgtacatc tcgcggagcc tgacaataag
     2941 cagatgacca agatatgtca gcaccttggc aaacaactca gaaatgaaca tgacattgat
     3001 tatgataatt tcttcaccga aacggaggtg aaaaagttta ttgatgccat gaatgcgaaa
     3061 aatgactcaa cagccgttca acctgtcgat tctgtcggca aaacaaatga taccaaaaca
     3121 agcaaatacg ataaaattaa aaaggaaaaa aatactatcg acgaaaaagc accttactgg
     3181 cgatttgcat ttgcatcggt agatcattcg gcagaattca aagcgctgtt tgaggaggaa
     3241 gaagaattgg ttgcaacaac ttctaaaaag aagaagaaat cggagttcaa tctcggatta
     3301 aaagaattgg tgatagtaga tcctgtttat atcagtatcg acgaacgaag taacaatcct
     3361 gtgcagtacg aagccgctga agatgctaag ctgaacctta aggataaagt gaaagcatcg
     3421 gcagacgaat taaacttgaa agtggaatac cttgaccata caaacatcga cgacagagat
     3481 gttgaaacct tcaacgaaat ggcagtgctt agtcgctgga tagaggagaa actgacacat
     3541 attgatgaag atatttttat cgagacttcc acaaatgacg aattgcagga aattgcaaaa
     3601 agcctcgata ttgatcattt tgcatggatg gggatcgtat catttaccga atcagaagag
     3661 tatgttggtg ctaagatatt tttgtgccta tatcttccat tggcaccatt cctgatttat
     3721 gaccttgtta cacctgatag aaatacattt tattttgcgc ttgtggcaaa tgcggaaaca
     3781 gggaaactcg agatgcaata ttttaacagc agcacactta atgattctaa tgctgcacag
     3841 agtagcaaca tttattacat tcttcagcaa atcaaaggtc gcaagaaatg aaaaggataa
     3901 ttttagcatt atgtagcctc gcaatttccg catcggcgat tgctcaggtg ccgggatata
     3961 tgggaaaacg cttgcagatt tatgcatctg ttgagccaac gtttgatttc agatacatca
     4021 ctgacggact tgattactat ttacaacaac ccgaagtaac cattccaacc gaatatggta
     4081 attatggaat tggtgttacg cccggagtgg gcatcgagta taccttgaac aacacatttg
     4141 taatgggtgc cgatgtgaaa tggaagcagg cgaaggcaca attgcatatg tattataaca
     4201 ccgcttctaa tccggatatt ccttatggat atctgggtga aggaaaaata tccggcatgt
     4261 taaccggatt ctatttcaaa atatatagtc atcgcaaaca tggttacatc gctcccattg
     4321 gcagatatca tcaattcgaa attttgaccg ggaaattttc ggtgcaagaa agtggatata
     4381 gttctacgga tttctatgct gttgactatc aggaatacta cagtaacctt agcttgcaag
     4441 cttcagaatt gacacccgtt gcgtcactgt cgcaattgaa ttttgatccg catagaatta
     4501 gttatttgcg ctatgtattt ggcggagaaa cagtgctttt cgacaaggtg ccggctgata
     4561 ttggtattca gtttgcaatg cctttatcct tcgttcagta tagtgattat gacaatggaa
     4621 tagataaggg cggatatgat tatgcttata ctgttcgcaa aagtatgaac ggtgcaatgg
     4681 cagtaggtat cgtgatgcgc tttggtatat tcgcgatttg atacatcatt acaatctgtt
     4741 ttttttt
//
LOCUS       DAOQOU010000214         4704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_11461_length_4704_cov_4.369972, whole genome shotgun sequence.
ACCESSION   DAOQOU010000214 DAOQOU010000000
VERSION     DAOQOU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11461_length_4704_cov_4.369972"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1946
                     /locus_tag="PLM90_13330"
     CDS             30..1946
                     /locus_tag="PLM90_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90413.1"
                     /translation="MKIHLKNPQILRKSRLRIIKLICLLFIFILGNISISIAQQFNWT
                     WVSQVHGTHMDEINAVKSNIRGELLVAGTFYSTAIQWQNTTLINPYENQHNGIYLGML
                     NTQGNLLWTNSIFCAPDTYISDAYIYDAELNNFGESFISGHVYRNGIIIDTTEILIDD
                     EYSSSGFITKFDAYGNLVWGHVFEDGCNVTSVVADTDGGFYIMGNMYWYCYSIDFGDT
                     ILENIPNGNQAYIAHYFSDNTLDWVKTVHGYISDIQGLLTQSNGLLVYGTYQINGYLD
                     TLTIDTLSIQCPFSTYYCSFWGQLNNKGEVQWLKNVAQEDLSVSNLYKTKEGYKRFVW
                     FEPPYIICGADTIFGETESYYAGAILSFDQQGDFENSNSVPIKLGNDFYQLYYSPNDE
                     CYAALLLYDSVLCQDAYIVNHALKNDPVILQMDTDFNSIDCYQQDLNYLSYDPAITWD
                     RFGNMILIGSFSADTLEFGDAILTNYQLQTQTDFYLTYGLRCDTTLLTIIYQNGVLQA
                     PPGESWDWYINYSKIPQETGPVMVPHQVGYYLASATQPDGCVKWTKPFWYDSDSKSVE
                     IFIYPNPTSGNVTILLPESITYCDIYNLEGQLVYKCTSEQQQNIDLRHLPSGNYYVKA
                     GNDSAIFASKFILL"
     gene            1956..2834
                     /locus_tag="PLM90_13335"
     CDS             1956..2834
                     /locus_tag="PLM90_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90414.1"
                     /translation="MERLNLCEYALLAASFLQLHKAQAEISYVDFDPDIVLDDPGELY
                     LLDFDTDGVEDFRFIHHKGVYFTYWGSQPRYFDAIFAGAVDDGNWIAGSYQTYGSAAY
                     SSYVTFHPYFIPLGYPIGVMLSFQEYYSQLVGAAIANADGTIIDYKGHWGEGAEGYLG
                     VRIERSLNYYYGWIRVTVADSSKSITIHDLAFENVAYDNIVAGDIFGNLPIESVLPNE
                     PILYCDGKDLYIDPGTYQNTSEHELSIYDLSGKLVLNQQLNNITPVIHLDVNPGIYIA
                     CYSDSYQRISAKIVIL"
     gene            3754..>4704
                     /locus_tag="PLM90_13340"
     CDS             3754..>4704
                     /locus_tag="PLM90_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788742.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HMZ90415.1"
                     /translation="MMDNQPYQPSHKVRIVTAASLFDGHDAAINVMRRIIQSTGAEVI
                     HLGHDRSVEEVVNCAIQEDVQAIAMTSYQGGHVEYFKYMYDLLKEKGASHIRIFGGGG
                     GTILPTEIEELHDYGISRIYSPDDGRAMGLQGMINDLVKKSDIPTGVNLNGGINKLGI
                     QDWGGIARLISAVENQTADAAGIMAQVEVMIKSSKTPVLGITGTGGAGKSSLVDELVR
                     RFLLDFKDKTIAILSVDPSKRKTGGALLGDRIRMNAINNSRVYMRSMATRQANLALSK
                     HVDEAVKILKAAHFDLIILETSGIGQSDTEITEHSDVCMYV"
BASE COUNT         1431 a          917 c         1013 g         1343 t
ORIGIN      
        1 atatttaata gatttgaaaa ccataagcca tgaaaattca tttaaaaaat ccgcaaatcc
       61 tccggaaatc cagactacgg ataattaaat tgatctgcct actctttatt ttcattttag
      121 gcaatatttc aatatctatt gctcagcaat ttaattggac ctgggtaagc caggttcacg
      181 gcacacatat ggatgagata aatgcagtca aatcaaacat tagaggagaa ttgttggttg
      241 caggcacatt ctatagtaca gccatacaat ggcaaaatac taccttgatc aacccttacg
      301 agaaccaaca caatggcata tatctcggaa tgcttaatac acagggaaat ttactctgga
      361 ccaattcaat attttgtgca ccggatacat acatctcgga tgcttacatt tatgatgctg
      421 aattaaacaa ttttggcgaa agttttataa gtgggcatgt atatagaaat ggtattatta
      481 ttgacacaac agaaatatta atcgatgatg aatatagctc aagcgggttt ataactaaat
      541 ttgatgcata tggcaattta gtgtggggac atgtatttga agatgggtgc aatgtaactt
      601 cagttgttgc agatacagat ggcggatttt atatcatggg gaacatgtac tggtactgtt
      661 attcaatcga tttcggagat acaatacttg aaaatatacc aaacgggaat caggcatata
      721 ttgcccacta ttttagtgat aacacccttg actgggtaaa aacagtgcat gggtatattt
      781 cagatataca gggacttcta acccaatcga atggactgct ggtttatggg acatatcaga
      841 taaatggtta tttagataca ctcactattg atacgctttc tatacaatgc ccgttttcaa
      901 cctattattg ctccttttgg gggcagctca ataataaggg tgaagtgcaa tggcttaaaa
      961 atgttgcaca agaggacctt agcgtttcaa atttatacaa aaccaaggaa gggtataaac
     1021 gatttgtttg gttcgagcct ccttatataa tatgcggcgc agatacaatt tttggtgaaa
     1081 ctgaatcata ctacgcaggt gcaattctgt cattcgatca gcaaggtgat tttgaaaatt
     1141 caaatagtgt tcctataaaa ctaggcaatg atttttacca attatactat tcacctaacg
     1201 atgaatgcta tgccgcattg ctactatatg attctgtatt atgccaagat gcctatatag
     1261 taaatcatgc gcttaaaaac gacccagtca tattgcaaat ggataccgat ttcaatagta
     1321 tcgattgcta tcaacaggac ctgaactatc tctcttatga ccccgcaata acatgggata
     1381 ggtttggcaa tatgatttta atcggcagtt tttcagcaga cacgttggaa ttcggtgatg
     1441 ccatactgac aaactatcaa ttacaaactc aaacggattt ttatcttacc tatggacttc
     1501 gatgcgatac cacattattg acaataatct atcagaatgg ggttctgcag gcgccaccag
     1561 gagaatcctg ggattggtat ataaattatt caaaaattcc acaggaaacc ggccctgtta
     1621 tggtgcctca tcaggttgga tattatttag caagtgcgac ccaacctgat ggttgtgtaa
     1681 aatggactaa acctttttgg tatgatagcg acagtaaatc tgtcgaaatt ttcatctacc
     1741 caaatcccac atctggaaat gtaactattt tgttacctga atcaataact tattgtgaca
     1801 tatataatct ggaagggcaa cttgtttata aatgcacatc tgaacaacag caaaacatcg
     1861 atcttaggca cctcccatca ggtaattact atgttaaagc aggcaatgat agtgcaattt
     1921 ttgcttctaa attcatcctc ttatagaata cccccatgga aagactcaac ctttgtgaat
     1981 atgcgcttct tgctgcatcc tttcttcaat tacataaagc acaagcagaa ataagctacg
     2041 tggatttcga tcctgatata gtattggatg acccaggtga actttatctg ttagattttg
     2101 acacagatgg cgttgaagat tttagattta ttcaccataa aggcgtatat tttacttatt
     2161 ggggcagtca accgcgctat tttgatgcaa tttttgcagg cgcagtggat gatggcaatt
     2221 ggattgctgg aagttaccaa acttatggaa gtgcagcata tagctcgtat gtaacatttc
     2281 atccttactt catccctttg ggttatccta ttggggtaat gttgagtttc caggaatatt
     2341 actctcaatt ggttggagct gcaattgcaa atgctgatgg aactattatt gattataagg
     2401 ggcattgggg agaaggcgca gaagggtatc taggggttcg gattgaacgt agtttaaatt
     2461 attattacgg gtggataagg gtgaccgttg ctgattcttc gaaatctatc acaatacatg
     2521 accttgcctt tgaaaatgtg gcatatgata atattgtcgc aggcgatatt tttggtaatc
     2581 tgcctattga atctgtatta cctaacgaac caattcttta ttgcgatgga aaagacctgt
     2641 atatcgatcc aggcacatat caaaacacca gtgagcatga attgagcatc tatgatttat
     2701 caggcaaatt agtcttaaat caacaattaa ataatataac tccagtaatc catcttgatg
     2761 ttaaccccgg tatctatatt gcctgttact ccgattccta tcaaagaata tctgccaaaa
     2821 ttgtgatatt ataagatggt ctatcattcc acttaaatct tgaaccaact gtataggtta
     2881 atcaacccta aatctaccat acctccatca aaacagatct ttcaaacaca gttttagaac
     2941 tttaaaccta cccaatcctc ctaacctgca acaataagtt tcaaaaggat ttgcttgaaa
     3001 tttatatcca taacgacaaa ggtctgccct gaagtgacaa aatgagcagc ggagtggagc
     3061 attttttcca cttcagggtg gtgattagaa tcttctcgaa atggagatac ttcattggcc
     3121 ccggaagtag aaaattttct tcgctgcgct acgaaaattt acacttcggg gcaggcttga
     3181 tttttagtac aaaacggtct gccctcagca catgcacgat tcacgacaca ccaatggcga
     3241 ggtactcgca cacctgttta aatcgcggag ttagcggagt tggcggagtt ttaggaatgg
     3301 atgcatcaaa ctctgcgcaa tctgtgggac ataaaacacg aaccacatgc acaaaatgca
     3361 gctgcacgca aaaccgctaa gtgcaaaagg aatgcaaaat ccaaaaattc accactcaca
     3421 actcaccact caccaatttc acccatccgc cttacggcga ggtagactca ctcacacgcc
     3481 tgtttaaatc gcggagttag cggagttttg ggctgaggcg tatccaactc tgcgaaaatc
     3541 tgcggcctct gcgcttaatt gcatttctgc aaggtttggc catgggacac gggtattaac
     3601 tgcacctgat tgtagcggaa agcccggaca ccgaggatac gagggggagg acttgcagcg
     3661 gaaagcagga ctgccgcccg gggagaacgg caagggtggt gcccatttcg agcatcaggg
     3721 taatatccgt aaatttgccg tatattctgc catatgatgg acaatcagcc ataccaaccc
     3781 agccataaag tgaggatagt gaccgctgcc agcctgttcg acgggcatga tgcggccatt
     3841 aatgtgatgc ggaggatcat tcagagcacc ggcgctgagg tgatacacct ggggcacgac
     3901 cggagtgtgg aggaggtggt gaattgtgcc atccaggaag atgtgcaggc cattgccatg
     3961 acctcgtatc aaggtggaca cgtggagtat ttcaaataca tgtatgattt gttaaaggaa
     4021 aaaggtgctt cgcatatccg catttttggc ggcggcggag gtaccatact tccgacagaa
     4081 atcgaggaat tgcatgatta tggtatttcg cgcatttatt cgcctgatga tggtcgcgcg
     4141 atgggattgc agggtatgat caatgacctg gtgaaaaaaa gtgatatccc taccggtgtt
     4201 aacctgaatg gcgggatcaa taaactgggt attcaggatt ggggtggtat cgcccgattg
     4261 atatcagctg ttgaaaatca aactgctgat gctgccggta tcatggcaca ggtggaagtg
     4321 atgatcaaaa gcagcaaaac gccggtttta ggtattacag gaaccggtgg agcaggaaaa
     4381 tcatcattgg ttgatgaatt ggtgcgcaga ttcttactcg atttcaagga taaaacaatt
     4441 gccattcttt cggttgatcc atctaaacgc aaaaccggag gtgcactgct cggtgacagg
     4501 atacgtatga atgccatcaa taacagtcgc gtgtatatgc gttcgatggc tacgcgtcag
     4561 gcgaatttag cattgagcaa gcatgttgat gaagcggtaa aaattctgaa agctgcacat
     4621 tttgatttaa ttattcttga gacatcaggc attggtcaaa gcgatacaga aattactgaa
     4681 cacagcgatg tgtgcatgta tgtg
//
LOCUS       DAOQOU010000215         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_11587_length_4674_cov_6.387530, whole genome shotgun sequence.
ACCESSION   DAOQOU010000215 DAOQOU010000000
VERSION     DAOQOU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11587_length_4674_cov_6.387530"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1579
                     /locus_tag="PLM90_13345"
     CDS             170..1579
                     /locus_tag="PLM90_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ90416.1"
                     /translation="MKYILLLSTLFSLQLRGQTVIDTTIYSREQLIEDIRYCRDFHEK
                     HNTNLYAYRSKAYIDSMFDDLEDKVVPMDAITFFKYMSIVTSSISDGHCLLFPSEEFR
                     TTADTVKGFFPFRIHYDDGAFYVTMDLSDSENAFSNGKLPIEAINGIPADSIYHAALR
                     SLPREGNNLQYPNWILEKYFYEYYSYIYGHPETFQLSTTCRIDDKLFEDCVLVEIPAV
                     TKDTLVERRKKRYPDYPMTLARDKGLSFTRYDSLDAAVLSLFTWDKTILRKVYGIHFK
                     DSINTYMQTAIDDSIGTLIIDLRNNQGGFMMYGVYVMRWLMDSTFNYIDKIERVHKTS
                     SNERKYKSVTTGRFKSVKPYKHNYGGNVIILTNGGTFSNSSIFTTQMQRYGRAVVMGE
                     ETGGSSSQLTGSFGVHRPLIMPNTKIELSHINYRIVVNKDVPYSGKGVIPDIAIADDW
                     NPENGDILLQKVLKEYLPK"
     gene            1652..2254
                     /locus_tag="PLM90_13350"
     CDS             1652..2254
                     /locus_tag="PLM90_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90417.1"
                     /translation="MQPLFPKSIETIIATVAGLLITSAQLILIRDVWRKKINPSLLTW
                     LGWGLIMGTGLLAQILEDGWQSSLTGLALSTAGCLCIFTIALLRNTFLYQRSDIKYLV
                     LGLLCLIIYISSHNAIITTLFAILADFIAGIPTLIAGYRNPKSQKTIAWTLGAVSWAF
                     TISVCFGHAWLYAWFPIYLFLYNLTLYIFTHRSTNAGKSI"
     gene            2295..2786
                     /locus_tag="PLM90_13355"
     CDS             2295..2786
                     /locus_tag="PLM90_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90418.1"
                     /translation="MTPRLNGQVFFYTNVIADIDKQVEKINDRKGNNGIRIAPWDSIQ
                     NFYDFNGPNIFGHFHFNVLEKAMIKIEEESGTQWTTTYYFKDNKLIHVEITCFRIAGF
                     SGPTWYELAGKYRYWFDHDAFISSDITLIPPDSPYQVSFTSAELLDQATCFADLLYKE
                     QEK"
     gene            3059..3850
                     /locus_tag="PLM90_13360"
     CDS             3059..3850
                     /locus_tag="PLM90_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90419.1"
                     /translation="MKKHVLLLALVAGLFSVQSLTAQITGFCFTDAFGYTTSVTATKT
                     GPGHWDLSGTADVLTGSDWIVTGSYDKMSDVWTLNFANPAPDGCTFYVDNFTYTSYAY
                     GGGVIYFNWTSYCFGGALGSGSGSTTWSLGTCPMRLGNETAIVGAAAADLTIANAPVA
                     DLRSIPGGELFSSAFTEAQFSVTRNADNTFSFGYTVDTESDVAIEIYNHAGQHIATIV
                     NGHMSNGFYVNNWDGKLANGTDALSGVYIAVMKSNGNMVTNKFVK"
     gene            3987..4499
                     /locus_tag="PLM90_13365"
     CDS             3987..4499
                     /locus_tag="PLM90_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90420.1"
                     /translation="MLLCVITLVGRNLSAQPTPQEIRAKEADKKVEVIKASKPTDSVV
                     LAAGDPLIKDIQPQTTSLKGYWYNGKLQLCIAKSIDASGNKWEVRYYFSDTSLYQIVS
                     DFYTVQTFISPDFFVWQFKKTFYLKYGEMFYYAADGTDRGMPPEYEDLALMQAYFKKY
                     YAAMNSAHTK"
BASE COUNT         1372 a          963 c          877 g         1462 t
ORIGIN      
        1 tctactggca aattatcagg tcacaccgct tggcaagatg catatggaag caaccgacct
       61 ttccggcaac tcagcagccg gagatctata tccaggtagc atttcactta ctgcaggcgt
      121 gcacattcca ttggtcaaat gatttaatat ctttgatcac tacatgtcca tgaaatacat
      181 tttactcctc tcaaccttgt ttagcctgca attgcgtgga caaaccgtta tagacacgac
      241 catttattcg cgagagcaat taattgagga tattcgctat tgtcgcgatt ttcacgaaaa
      301 acacaatacc aatttatatg cctatcgttc aaaggcatat atcgacagca tgttcgatga
      361 tctcgaggat aaagttgtgc caatggatgc cataactttt ttcaagtata tgtccattgt
      421 tacatcttcc atttcagatg gtcactgctt attatttcca tccgaggaat tcagaacaac
      481 agcagatact gtgaaagggt tctttccatt tcgcatacat tatgatgacg gtgcatttta
      541 tgttactatg gacctttcag acagcgaaaa tgcattttca aacggcaagt tacctattga
      601 ggccattaat ggtatacctg cagattccat ctatcatgct gcactgcgct cgctgccgcg
      661 ggaaggaaat aatttacaat atccaaattg gatcctcgaa aaatattttt acgaatatta
      721 tagttatatc tacggtcatc ccgaaacgtt tcagttaagc actacatgta gaatagatga
      781 taaattgttt gaagattgcg tgcttgtaga aatacccgct gtcacaaaag acacattagt
      841 agagcgaaga aaaaaacgat accccgacta tccgatgaca ctggcgcgtg acaagggttt
      901 gtcgtttacg cgatacgatt ctttggatgc agcagtgctt tcattgttta catgggataa
      961 aacaattctg cgaaaagtat atggtattca ttttaaagat agcattaaca catatatgca
     1021 aacagcaatc gacgattcca ttggaacttt aatcatcgat ttgcgcaata atcaaggtgg
     1081 attcatgatg tatggtgttt atgtaatgcg ctggttgatg gatagcacct ttaattacat
     1141 tgataaaatt gaacgggttc ataaaacttc ttccaacgaa cggaaataca aatctgtaac
     1201 taccggtcga ttcaaatcag tcaagcccta taagcacaat tacggcggaa atgtaatcat
     1261 acttaccaat ggcggcactt tttccaacag cagtattttc accacacaaa tgcaacgtta
     1321 tggtcgcgcc gttgtgatgg gcgaggaaac aggaggcagt tcatcgcaat taacaggcag
     1381 ttttggcgta caccgaccgc tgataatgcc aaatacaaaa attgaattat cacatatcaa
     1441 ttatcggatt gtggtaaata aagatgtgcc ctatagcgga aagggagtga ttcctgacat
     1501 cgctattgca gatgattgga atccagaaaa tggtgatatc cttttgcaaa aggtgctgaa
     1561 ggaatattta cctaaataag catgtttcgc atattgaata tgttatattc tacttctgaa
     1621 taagattatt taccgaactt ggcatcttaa gatgcaacca ctctttccca aatcaataga
     1681 aaccatcatt gcaactgttg caggattgct catcacatcg gcacagctta ttctgattcg
     1741 cgatgtatgg cgaaagaaga ttaatccctc actgctcacc tggctgggat ggggtttaat
     1801 catgggaaca gggttgctgg cacaaattct cgaggatggc tggcaatcga gccttacggg
     1861 cttggcatta tctaccgcag gttgtttatg catatttacc attgctcttt tgcgcaatac
     1921 gtttttatat cagcgcagcg atattaaata tctggtgctc gggcttttat gtttgattat
     1981 ttacatcagc tcacataacg caatcatcac tacactgttt gccatacttg ccgattttat
     2041 tgcggggatt ccaacactta ttgcaggata ccgcaatccg aaatcacaaa aaaccattgc
     2101 gtggacacta ggtgccgttt cctgggcgtt tactatcagt gtttgcttcg gacacgcatg
     2161 gctatatgcc tggtttccga tttatctttt tctatataac cttaccttat atattttcac
     2221 acaccgatcg acaaatgccg gcaaatcaat ttaaatatct tctgcatggt atttttatta
     2281 ccttgctatt gttgatgacg ccccggctca acggtcaggt atttttctat acaaacgtaa
     2341 tagcagatat agataaacaa gttgaaaaaa ttaatgatcg caaagggaac aatggcatcc
     2401 gcattgcacc ttgggattct attcaaaatt tctatgattt taacgggcca aatatttttg
     2461 gacattttca ttttaatgtt cttgaaaaag cgatgattaa aattgaagaa gaatccggta
     2521 ctcaatggac aactacatac tatttcaaag acaacaaact tattcatgtt gaaattactt
     2581 gcttcagaat agcaggattt tccggaccta catggtatga acttgcgggt aagtaccgtt
     2641 attggttcga tcatgatgca ttcatttcat ccgatattac attaattcca cctgattcac
     2701 cttaccaagt atcttttaca tccgcagaat tactcgatca ggctacttgc tttgccgatt
     2761 tgctttataa ggaacaagaa aagtgattaa ttaaggcacg gaaaaccgtg aattttaaaa
     2821 taattcactt ttttttgcat ttgcgatttt caataattta acatttggcc tgaagtgaat
     2881 ttacatggca agttatgcta tagaactact caatttaatt atttgcagat atgacaattg
     2941 ataaatttag tgtttctgat attacaaatc atacaaaaat taatgtcgac taatttgaat
     3001 ttgtgacatg cattttctag ctttatgaaa tcaatttaat caccaaaaac atgttatcat
     3061 gaaaaaacac gtcttactcc ttgcccttgt ggccggcttg ttcagtgtac aatccctgac
     3121 agcacaaatc accggttttt gttttactga cgcttttgga tacactacaa gcgtaacagc
     3181 aactaaaacc ggcccgggac attgggatct ttcaggaact gctgatgtac tcacaggatc
     3241 tgattggatc gtaaccggtt cttacgacaa aatgtctgat gtttggacat tgaattttgc
     3301 taaccctgca cctgatggat gtactttcta cgtagacaac ttcacgtata catcttatgc
     3361 ttacggtggt ggcgttattt acttcaactg gactagctac tgctttggcg gtgcactcgg
     3421 aagtggttca ggtagcacta cttggtcatt aggtacttgc ccaatgcgtc ttggtaatga
     3481 aacagctatc gtaggtgctg ctgctgcaga tcttacaatt gctaatgctc ctgttgctga
     3541 tcttcgctct atccccggcg gtgaactttt cagctcagct tttacagaag ctcaattcag
     3601 cgttacacgt aatgccgaca acactttctc tttcggttat actgtagata ctgaatcaga
     3661 tgttgctatc gaaatttata accatgccgg tcagcacatt gctactatcg tgaatggtca
     3721 tatgagcaat ggtttctatg taaataactg ggatggtaaa cttgcaaatg gtactgacgc
     3781 cctttcaggt gtttacattg ctgttatgaa atctaacggt aatatggtta ccaacaaatt
     3841 tgtgaaataa gcgcaagcac acttcacaca aaaaaaacgc aacctatcat gggttgcgtt
     3901 ttttttgtgc ctatttttga agctcctatc ataatacatc gatgtttttc gttatagacc
     3961 atatgaagca atgctacaca cgattaatgc ttctctgcgt cattacactt gtcggccgga
     4021 atctttctgc tcaacccact ccgcaggaaa ttcgggctaa agaggccgac aagaaagttg
     4081 aagtgatcaa agcctcaaag cccacagaca gtgttgtgct tgctgcaggc gatccgctga
     4141 tcaaagacat ccaaccacaa accacttcat taaaaggcta ttggtataat ggcaaacttc
     4201 aactttgtat tgccaaaagt attgatgcaa gcggtaataa atgggaagta cgttattatt
     4261 tctccgacac ctcattgtac cagatcgtat ctgattttta taccgtgcaa acatttatca
     4321 gtcctgattt ctttgtgtgg cagttcaaaa aaacattcta cctgaaatat ggtgaaatgt
     4381 tttattatgc tgccgacggt acagatcgcg gtatgccgcc ggaatatgaa gaccttgcac
     4441 tcatgcaagc ctacttcaaa aaatattatg ctgctatgaa tagcgcccat acgaaataag
     4501 aattttcata ttctatttca gtatccttct atttcattct ttcactccta aaccattttg
     4561 tatgcagttt acttcacgca ttacatcgtt cttatgcatt tctttgattg ccatttctgt
     4621 ttgccacgca caaagcattt ctctttattt cgctgtcgac gatgcaacat taac
//
LOCUS       DAOQOU010000216         4570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_12053_length_4570_cov_5.207087, whole genome shotgun sequence.
ACCESSION   DAOQOU010000216 DAOQOU010000000
VERSION     DAOQOU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12053_length_4570_cov_5.207087"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLM90_13370"
     CDS             complement(<1..322)
                     /locus_tag="PLM90_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90421.1"
                     /translation="MKLRLLLTVFSLGAFAAFGQITLTPSTAELDGDPSVIQEIHIDF
                     HNASGAKQTMTWLRTMNDIPDEWTTSVCDFNLCWAPTADEPGYYFDAPIDTTGTVYVK
                     FDARN"
     gene            complement(451..2502)
                     /locus_tag="PLM90_13375"
     CDS             complement(451..2502)
                     /locus_tag="PLM90_13375"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ90422.1"
                     /translation="MKRFFYSLPVVALLFVTATAFGQAKKYPLFEHFTQASCGPCAAQ
                     NPYFQAVYYANESNLHHVAYHTSWPGIDPMYDFNSAESDAMVDYYGVTGVPDMIQNGS
                     DIGSPAGVTQDIVNNLLSETSPIRILVTESTTGTTRNVHIEVVTVGTVPAGTYKLKAA
                     VVEHIVDYVTAPGTNGETEFPNVFRESITGTSGVAITPAAIGSSVSFDYSYELDPIYV
                     DENIYALAWVQNSSTKEVLNSGSASDPDVEVINTSAATFVQGTVGGATPFTGNILNLN
                     DAEGMNMHFEFAADQPGDWSASYTYDGTTYTGATDAYIAGAGDKDVTFNINVGASAAI
                     GEYYLTVTFPDNPEISAQVLSYYVISGVTDLVVNNEVSFGDGSAYGTYDWESLYVDGL
                     TAAGVSTVAATSHFTMKKGFNSGALDGIDNIYYNVGWTFPGLVDDAEKIAQLMDFLDN
                     GGNLFISGQDIGWEVNEYAAYYPEAVDFYENYLMASFVNDAASGATVFSPVLTDEWYG
                     AVAESDISKPYGTTYYYPEQIEPNGVDATSVFTYNAGTKVGGVRAETSDFKTVYFGIG
                     IEMIADDAVRNAVMAATYAYFRGDLEGIDYDALISGLMGGAQPNPAVNSTVIPMTNIN
                     SDYQLTVTDLTGKVILTDIVKAGTSSYTLNTADLSAGMYLYYMSSGNSRTQTEKLNVV
                     K"
     gene            2667..4067
                     /locus_tag="PLM90_13380"
     CDS             2667..4067
                     /locus_tag="PLM90_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ90423.1"
                     /translation="MFTKLNAAHIARLTEIVGAAYAFLGNDQLLEYGSDETEDLSFPP
                     EIVLKPRTPEQISEILKFANDHMIPVTPMGARTGLSGGALPVFGGIALSMERFNEILH
                     IDERNLQVTTEPGVITEVLQNAVRDKGLFYPPDPASRGSCFIGGNIAENSGGPKAVKY
                     GVVKDYVLNLEVVLPTGEIIWTGANVLKNATGYNLTQLLVGSEGTLGVITKIVLRLIP
                     HPQRDMLLLIPFPSAVKACAAVAEIFKAGITPSAMEFMEKDAILYVLKHNPDLHLDLP
                     EGIEAHLLVEVDGNNPDLLMSEIEAISGIVEQFECGEVLFAESEREKQDLWRIRRKVG
                     EAVKQHSVYKEEDTVVPRAELPQLLAGVKEIGAKYGFQSVCYGHAGDGNLHVNILKGN
                     MTDASWDNTLSIAITEIFQLVKQLGGTISGEHGIGLVQKPYMNIVFDEVHLNLFRMLR
                     KVFDPKGILNPGKVVD"
     gene            4234..>4570
                     /locus_tag="PLM90_13385"
     CDS             4234..>4570
                     /locus_tag="PLM90_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90424.1"
                     /translation="MTRGKDILISILCMVCIGTTMRAQEFNGLRTDNYAGSYGMLLNP
                     AMPITGKIPWDVNFVALGVNEDNNYIKISDPVFKYVTTSDSILDVEYYNPTQIRGHGN
                     VLLQLPSAFI"
BASE COUNT         1333 a          986 c          971 g         1280 t
ORIGIN      
        1 aatttcttgc atcaaatttc acatataccg ttccggttgt atctattggc gcatcgaaat
       61 aataccccgg ctcatctgcg gttggggccc agcacaagtt aaaatcacaa acggaagtgg
      121 tccattcatc aggaatatca ttcatagtac gaagccaggt catcgtttgt ttagcaccac
      181 ttgcattgtg aaagtcgatg tgaatctcct gtattactga agggtcacca tccagttctg
      241 cagtagaagg agtgagcgta atttggccaa aagcagcaaa tgcgcccagc gaaaatactg
      301 taaggagtaa tctaagtttc atgataagag ttgataatat tgttaacggt ttattgtggt
      361 gctccgttgg tttatactac aaaaatagtg cagaataggc aaaaaaaaga gccaggttga
      421 tgtacctgac tcttttatga cgtattaaga ttactttaca acgttcaatt tttcagtttg
      481 agtacggctg ttaccggaac tcatgtagta aagatacata cctgcgctga ggtcagcagt
      541 attcaatgta tagctgctgg tgcctgcttt aacgatatcg gttaaaatca ctttgccggt
      601 cagatctgtt actgtaagtt gataatcgct gttaatattg gtcattggaa taactgtgct
      661 gtttactgca ggatttggct gcgcgccacc cattaaacca ctaatcaacg catcataatc
      721 aatgccttcc aaatcgccac ggaaataagc ataagttgca gccattactg cattgcgaac
      781 tgcatcatca gctatcattt caataccaat accgaagtat acagttttga aatctgaagt
      841 ttccgcacgc acaccaccaa cttttgttcc tgcattgtaa gtaaatacac ttgttgcgtc
      901 tacaccattt ggttcgattt gctcaggata ataataggtt gttccataag gcttgctgat
      961 atcactttca gcaaccgcac cataccattc atcagttaat accggactga atacagtagc
     1021 acctgaagcg gcatcattta caaaacttgc catcaggtaa ttttcataaa aatctacggc
     1081 ctcaggatag tatgcagcat attcatttac ttcccatcct atatcctgac cggaaataaa
     1141 taaattacct ccattatcga ggaaatccat caattgtgca attttttcgg catcatcaac
     1201 aagtccgggg aatgtccatc caacattgta ataaatgtta tctataccat ccaaagcacc
     1261 gctgttgaat ccttttttca ttgtgaagtg actagttgca gcaactgtgg aaacacctgc
     1321 ggcggtaaga ccatctacat acaacgattc ccagtcgtaa gtgccgtaag cagagccatc
     1381 gccgaaactt acttcattgt ttactactag atcagtaaca ccactgatca cataataact
     1441 caatacctgt gcactgattt ccggattgtc agggaaggta actgtcaaat aatactctcc
     1501 gatagcagcc gatgctccta cgttgatatt aaacgttaca tctttatcac ctgctccggc
     1561 aatgtaagcg tcagtagcac cggtataggt tgttccatca taggtatatg atgcagacca
     1621 atcgccgggt tgatcggcag caaattcaaa atgcatattc attccttcag catcattcag
     1681 attcaggata tttcctgtaa atggtgtcgc accaccaaca gttccttgaa caaatgttgc
     1741 agcagaagta ttaatcactt caacatcagg atcacttgca gaacctgaat ttaaaacctc
     1801 ttttgtcgat gaattttgaa cccatgctaa tgcataaata ttttcgtcta cataaattgg
     1861 atctagttcg tagctgtaat caaatgaaac acttgatcct atagctgcag gtgttattgc
     1921 aacaccactt gttcctgtaa ttgactcacg gaaaacattt ggaaattcag tttcaccatt
     1981 tgttccggga gcagtaacat aatcaacaat atgctcaaca acggcagctt tcaatttgta
     2041 tgtgccggca ggaacagtac caactgttac cacctctata tgcacattgc gggtggtgcc
     2101 tgttgtcgat tcagttacca atatgcgaat cggacttgtt tcagataaaa ggttgttcac
     2161 aatatcttgt gttacacctg caggacttcc gatatcagat ccgttttgaa tcatatcagg
     2221 aacgccggta acgccataat aatcaaccat ggcatcgctt tcagctgagt tgaaatcata
     2281 cattgggtct atgcccggcc agctggtatg ataggcaaca tggtgtaagt tagattcatt
     2341 ggcataatat actgcctgga aataagggtt ctgtgcagca caaggtccac acgatgcttg
     2401 ggtgaagtgc tcaaataatg ggtacttctt cgcctgccca aaagcggtag ctgtcacaaa
     2461 taatagcgct actaccggta aggagtaaaa aaatctcttc atttctgatg tagtttgaat
     2521 attaaagata cgccttcctc ctaaatcagc aaaacatttg ggaggaatga acctaatttg
     2581 acagatgtca agcatcagta aatgctaacg cagggtggtt ggcaattctc catgacaatt
     2641 ctaatgaggt ttataccttt gatctcatgt tcaccaagct gaatgcagcc catatcgccc
     2701 gactgaccga aatcgttggt gctgcttatg ccttcttggg aaatgatcag ttgttggaat
     2761 atggaagtga tgaaacagag gacctctcat tccctccgga gattgtctta aaacctcgca
     2821 ctccggagca gatttctgaa atcctgaagt ttgccaatga ccatatgata cctgtcaccc
     2881 ccatgggcgc acgcacaggg ctaagcggtg gtgccttgcc ggtttttgga ggtattgcac
     2941 tgagtatgga gcgtttcaat gaaatcctac atattgatga aagaaatctg caggtaacca
     3001 cggaaccggg agtaataaca gaagttttgc aaaacgctgt gcgggataaa ggcctattct
     3061 atccccctga ccctgcaagc agaggaagct gcttcatagg tggaaacatt gccgaaaatt
     3121 caggaggccc caaagccgtg aaatacggcg tggtgaagga ttatgtgctc aacctcgagg
     3181 tcgtattgcc aaccggagag atcatttgga ctggtgctaa tgtgctgaaa aatgctacag
     3241 gatataatct tacccaattg cttgtaggca gcgaaggaac acttggggtg atcaccaaaa
     3301 tcgttctccg gctgatacct caccctcagc gcgatatgtt actgctgatc ccattcccca
     3361 gcgcagtaaa ggcttgtgcc gctgttgcag aaatttttaa ggctggaatt acccctagcg
     3421 caatggaatt catggagaag gatgccattc tttatgtgct gaaacacaat cccgacctgc
     3481 atctcgacct tcctgaaggc attgaggcac atttgctggt tgaggtggat ggcaataacc
     3541 cggacctgtt aatgagcgaa atcgaagcaa taagcggcat cgtagagcaa tttgagtgtg
     3601 gtgaagtgct gtttgccgaa tctgaacgcg aaaagcagga cctctggcgt atcagaagaa
     3661 aggtgggtga ggccgtgaag caacattctg tttataaaga agaggatacc gtggtgcctc
     3721 gcgctgaact acctcaattg ctggccgggg tcaaggaaat tggtgcaaaa tatgggttcc
     3781 aatctgtttg ctatggtcat gccggcgatg gaaatcttca cgtgaatatt ttaaagggca
     3841 atatgacgga cgcctcgtgg gacaacactt tgagtattgc aatcacagaa atatttcaat
     3901 tagtaaagca acttggcggc actataagcg gcgaacatgg aatcggcctg gttcaaaaac
     3961 cttatatgaa cattgtattc gatgaagttc acctcaacct attccgaatg ctcagaaaag
     4021 tgttcgaccc taaaggcatt ttaaacccgg gtaaggttgt tgattgacaa gtcctcaata
     4081 aattattgaa atttctgcga aaaagtctgt tttttgtcat aatttttaca caactttatc
     4141 cctgtgaatc cgttttatgt gaaaagcgga ttacatgcag cacacacaag cagagctagc
     4201 gagagcacac acagcgacta acctgtttac tgcatgacaa gggggaaaga catcctgatt
     4261 tctattttat gcatggtgtg cataggaacc actatgcgtg cacaggaatt taacggtctg
     4321 cgcaccgata attatgccgg gtcttacggt atgcttttaa accccgcgat gccgattacc
     4381 ggcaaaattc catgggatgt aaattttgtc gcattaggtg tcaatgaaga caataattac
     4441 attaaaataa gtgacccggt tttcaaatat gtaactacct ccgattcgat attggatgtt
     4501 gaatattaca atcctacaca aattcgtggt catggaaatg ttttattgca attgccttct
     4561 gcatttatca
//
LOCUS       DAOQOU010000217         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_12340_length_4504_cov_5.105866, whole genome shotgun sequence.
ACCESSION   DAOQOU010000217 DAOQOU010000000
VERSION     DAOQOU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12340_length_4504_cov_5.105866"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLM90_13390"
     CDS             <1..508
                     /locus_tag="PLM90_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HMZ90425.1"
                     /translation="DNISNNMLIGAVNFFNDKTDSVKNQLNGQYDAVPAVQRNYKANG
                     VGSIVVGDENYGEGSSREHAAMEPRFLGVRAILVKSFARIHETNLKKQGMLALTFADK
                     NDYDKIQEDDNIDIIGLTTFSPGQPLTLRLHHSNGSSDDIIVNHTYNAQQIEWFKAGS
                     ALNLIGDK"
     gene            690..1979
                     /locus_tag="PLM90_13395"
     CDS             690..1979
                     /locus_tag="PLM90_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90426.1"
                     /translation="MKSRLITCMLFFCSMTLQAQNNSTCSFHGVVVIGSEEALLQGAY
                     LKCLGTGDDVITDDEGNFSLRVSCSGPDTLILQSISGSEKFLLQDGKYTDDTLRIVFK
                     LYNLNDIFVTPKEPVDIVRKAVARIPINYPETSHVAFGFYRNYKLINNRFRELTELQM
                     AVAMRFEEKGQHNELREAYAVDKIRRTLYTISIDEFYKGQLTDLFTQNLVYHTDFAFF
                     NNIFIKQCDFSFDSTSTDSTWVINYKLTNITGENHGVSNYSPVDFYGEATETGYLVIN
                     KSDYAIIKLVRESLRNKKYEYPKRNNFLLPDLQYSEQFHEGFLECNYAMFDGKYYPVS
                     IFHAYKNTFTHNPTNSIAYIITDYSEWHCDSISEMIDPVWMQRFDKFTSLERMYYTYD
                     PNDWTQTPSWYFIRNEQVYDDLQLIDDPEILFKKSGE"
     gene            2140..3615
                     /locus_tag="PLM90_13400"
     CDS             2140..3615
                     /locus_tag="PLM90_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90427.1"
                     /translation="MKNRLLLIVLTGLLSVNAQAQWMLDSANTAGTYPYAAVNGNKAV
                     FSNGGEWNVFDAVTGVHTYGNFAISRAMIEVVSAGDKVMFAGGKYGYFADPQYTKSVN
                     VYNATTNTWSTWNMGTAREVGGAAAIGGKAVFAGGTGRSDIAGPVYMYNKVDIFDVAT
                     GARTSGKLSKGRSNIAAGAAGNKIVFAGGWYWDMMYSVLPANTVDIYDVVTGVWTKTT
                     LSKKRENIAVAVIGDKILFAGGTGSMGAVTNVDIYNSTTNTWSVSYMPAAGDGMKSAV
                     IGSDAYFGGGITGPGNMLYKYNSVAGTWSSITIPTSLTGFSMSVINGRLIIAGGTIPG
                     TSTYSDLVQIYDPVANSWYNEHLSIARTNVSALTIGSKGYFAGGISVYGYPTPTYTKR
                     VDIFNAPFRMEQSIASEISNWDVQVYPNPAVNTVHIAIQDAFDQALIATITDINGNLI
                     QQFQLDATVQNIDVSELPSGIYFIIVQDNSARSIVKKLVKQ"
     gene            complement(3667..4428)
                     /locus_tag="PLM90_13405"
     CDS             complement(3667..4428)
                     /locus_tag="PLM90_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90428.1"
                     /translation="MKLILSILLLSLAANSFGQNLEPYNAEFKFQVVTGNNALRADST
                     VEAIIIVCDSVSIKNAYHSVEENLQIKELKLINPDQMLINEFASLELKNLHYLFIDAY
                     RHESLQISKFPNVEVFSVTSERLEKLDMVGAQFQKLAVLGVHAVELREWTSDSALPSL
                     ELLDIMAPHLDSLPILLMPQLYEFVFFCSFTSMPKDLCKYEELSLMSFNNFTAIPVDD
                     CIIEKIKSGVYSNLTVFESISGKELLKVVSNDRKE"
BASE COUNT         1447 a          862 c          897 g         1298 t
ORIGIN      
        1 cgataatatt tcaaataata tgctcattgg ggccgtgaat ttcttcaacg ataaaactga
       61 ttcggtaaag aatcaattaa acggtcaata tgacgccgtt cctgccgttc aacgcaatta
      121 caaagcgaat ggtgttggca gtattgtggt tggtgatgag aattacggtg aaggatcatc
      181 acgtgagcat gcagcaatgg aaccgcgttt ccttggcgtg cgcgctatac tggtgaaatc
      241 atttgcacgt atccacgaaa caaaccttaa aaaacaaggt atgcttgcat tgacatttgc
      301 cgataaaaat gattacgata aaattcagga ggatgacaat atcgatatca ttggtctgac
      361 tacattttca ccgggtcaac cattgacatt gcgtctgcat cacagcaatg gcagcagtga
      421 tgatatcatt gtaaatcata cctataatgc gcaacaaatc gaatggttca aggcaggaag
      481 tgcactgaac ttgattggcg ataaataaaa taatatccaa ctatactaaa aagagtgttc
      541 atacggacac tctttttttt tggatggtga tgaagaaaac ttgaagcagg catacaattt
      601 gtttcagaat ataaattcac atacgtcagc gcaatgtcag aattggtggc aagcgaaaca
      661 aattaaagaa ctactcgtaa attcgtttaa tgaaatcacg ccttatcaca tgcatgctgt
      721 ttttttgctc tatgacattg caggcgcaaa acaatagcac atgcagtttt catggggttg
      781 ttgtgattgg cagtgaggaa gctttgttgc aaggtgcata cttgaaatgc ctgggcacgg
      841 gcgatgatgt aattacagat gatgaaggaa attttagttt gcgcgttagc tgttccggac
      901 cggacacgct cattttacaa agtatcagcg gaagcgagaa gttcttactt caagatggaa
      961 aatacactga tgacacattg cgtattgtgt ttaaattata caacctgaat gacatttttg
     1021 tcacgccaaa ggagccggta gacattgtgc gaaaagccgt tgcgcgcatt ccgataaatt
     1081 acccggaaac ttcgcatgtg gcatttggtt tttacagaaa ctacaaactc ataaataaca
     1141 gattcagaga attaacggag ttacaaatgg ctgttgccat gcgatttgaa gagaaaggcc
     1201 agcataatga attgcgcgaa gcatatgcag tagacaaaat ccgcagaaca ttatatacga
     1261 taagcataga tgaattttac aaagggcagc ttaccgatct gtttacccaa aacctcgtat
     1321 atcatactga ttttgcattt ttcaataata tcttcattaa gcaatgtgat ttcagtttcg
     1381 acagcacttc tactgacagc acatgggtaa ttaattataa gcttactaac atcactggtg
     1441 agaatcatgg tgtaagcaat tattcaccgg tagattttta cggcgaagct accgaaacag
     1501 gatatcttgt tattaataaa tcggattacg ctatcataaa acttgtacga gaaagtctaa
     1561 gaaataaaaa atacgaatat ccaaaacgaa ataattttct gttacctgat ctccaatatt
     1621 cagaacaatt tcatgaaggc tttttggagt gtaattacgc aatgtttgat ggcaaatatt
     1681 atccggtgag tatttttcat gcgtataaaa acacttttac acataaccct accaatagca
     1741 tagcctatat tatcaccgat tattccgaat ggcattgcga ttcaataagc gaaatgattg
     1801 atccggtttg gatgcagcga ttcgataaat tcacgtcact tgagcgcatg tattacacct
     1861 atgacccaaa tgattggaca caaaccccat cctggtattt tatccgcaat gaacaagttt
     1921 acgacgattt gcaactgata gacgatccgg aaatactctt caaaaaaagt ggagaatgac
     1981 cttccattaa ggacattaat tcacctgcgc cctacccttc tatttgtctg aaaagtgccc
     2041 tgaatcagca ttttgtgtga cataagtcat agcgcctctc cttgttttgc ttgcatcttt
     2101 gcttagggaa attttattga atcatttaaa actcaagata tgaaaaaccg acttctctta
     2161 attgtattga caggcttatt atcggtcaat gcacaggcac aatggatgct ggattccgct
     2221 aatacggcgg gcacttatcc gtatgctgcc gttaatggca acaaagcagt attctctaat
     2281 ggtggcgaat ggaatgtatt cgatgctgta accggtgtgc atacttatgg caatttcgct
     2341 atttcccgcg ctatgatcga agtggtaagt gccggcgata aagtgatgtt tgcaggtggt
     2401 aaatacggat attttgctga tcctcaatac acaaaatcgg taaacgttta caatgcgact
     2461 accaatacct ggtcgacatg gaatatgggt actgcacgag aagtaggagg tgctgcagcc
     2521 attggaggca aagcagtatt tgcgggtgga acaggtcgat cagacattgc aggacctgtt
     2581 tacatgtata acaaagtaga tattttcgat gttgccacag gtgcccgaac ttcaggaaaa
     2641 ttatcaaaag gaagatcaaa catcgctgca ggtgctgcag gaaataaaat tgtgtttgcc
     2701 ggcggatggt actgggatat gatgtatagt gttttacctg caaatacagt agatatttac
     2761 gatgttgtaa caggcgtttg gacaaaaacc acgctgtcga aaaaacgaga aaatattgca
     2821 gttgccgtta ttggcgataa aatcttgttt gccggtggaa caggttcaat gggagcagtt
     2881 actaatgtcg atatttataa ttcaacaacc aatacatggt cggtaagcta tatgcctgct
     2941 gcgggcgacg gtatgaaatc cgcagtcatt ggaagtgatg cttatttcgg aggtggcatt
     3001 acaggtcctg gcaatatgct atacaaatac aatagtgttg caggaacatg gagttctatt
     3061 actattccga catcacttac cggattcagc atgtccgtta ttaatggtcg cttgatcatt
     3121 gccggcggaa ccattcccgg aacaagcact tacagtgatc tggttcaaat ttatgatcca
     3181 gttgcaaaca gctggtataa tgaacatctg agcattgcac gcacaaacgt atctgcattg
     3241 accattggca gtaaaggata ttttgcggga ggtatttcag tatatggtta tccaacccca
     3301 acctatacca aacgcgttga tatatttaat gcaccgttta gaatggaaca atcaattgca
     3361 tccgaaatca gtaattggga cgtgcaagta tatccaaatc ctgcagtaaa caccgttcac
     3421 attgccatac aagatgcatt tgaccaagca ttgattgcaa ctattaccga tatcaatggc
     3481 aatctgatac aacaattcca attagatgcc acagttcaaa atatagatgt cagcgaatta
     3541 ccttccggca tttattttat aattgttcaa gataacagcg cccgttccat tgtaaaaaaa
     3601 ttggttaaac aatgaccggg aactaagtgt agtccataca aagagccatc cttcggggtg
     3661 gcttttttat tcctttctat cattggaaac cacttttaac aactctttcc cggagatgct
     3721 ttcgaaaaca gttagattag aatacacacc tgatttaatt ttctcaatga tacagtcgtc
     3781 aaccggaata gctgtaaaat tattaaacga catcagactt aattcttcat acttacacag
     3841 atcttttggc attgaagtga aggagcaaaa aaagacaaat tcatacaact gaggcattaa
     3901 aaggattggt aatgaatcaa gatgaggagc catgatatct aacagttcaa gcgaaggcaa
     3961 agcactatct gaagtccatt cacgcaattc tactgcatgc acaccaagaa cggctaattt
     4021 ctgaaattga gcgcctacca tatccaattt ctccaatctc tccgaagtaa cagaaaacac
     4081 ctcaacatta gggaatttgg aaatctgcaa tgattcgtgc cggtaagcat caataaacaa
     4141 ataatgtaaa ttttttaatt ccaatgacgc aaactcatta atcaacatct gatcagggtt
     4201 aatgagtttc aattccttta tttgcaaatt ttcttcaaca gaatggtatg catttttaat
     4261 gcttacagaa tcgcacacaa tgattattgc ctccaccgtg gaatctgctc tcaatgcatt
     4321 attgcctgta actacttgaa atttgaattc tgcattatat ggctctaaat tttgtccaaa
     4381 tgaattcgct gcgagcgata acaataaaat cgataaaatt aatttcatgg tcagttttta
     4441 atttatagca aataaaacct aatatgattt taaaacccct gccatcggtt caaaatctga
     4501 taga
//
LOCUS       DAOQOU010000218         4500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_12357_length_4500_cov_5.159505, whole genome shotgun sequence.
ACCESSION   DAOQOU010000218 DAOQOU010000000
VERSION     DAOQOU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12357_length_4500_cov_5.159505"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLM90_13410"
     CDS             complement(<1..926)
                     /locus_tag="PLM90_13410"
                     /inference="COORDINATES: protein motif:HMM:NF010177.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HMZ90429.1"
                     /translation="MIIEPRMRGFICLTAHPEGCAKNVSNQIAYVRSKGAIAGAKKVL
                     VIGASTGFGLASRISAAFGSGAATIGVFFEKAPQEGKTASPGWYNSAAFQAEAEKAGL
                     YAKSINGDAFSNEIKTKTIDTIKADLGKIDLVIYSLASPVRTHPVTGVTHRSVLKPIG
                     STFKNKTVDFHTGIVSEISIEPCNEEDIQNTIAVMGGEDWEMWMDALLKADVLADNAM
                     TIAYSYIGPSLTEPVYRKGTIGRAKDHLESTAGVITNKLQSVHGKAFVSVNKALVTQA
                     SSAIPVIPLYISLLYKVMKENGIHEGCIEQMQR"
     gene            complement(1007..1360)
                     /locus_tag="PLM90_13415"
     CDS             complement(1007..1360)
                     /locus_tag="PLM90_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ90430.1"
                     /translation="MEFKVEKKTGQVIVIHLKGRMIGEYETIPLNEQLEDLIEENFVR
                     IIFDLKELEYMNSSGLNFFLKTLTKVRRLDGEVVICAMNNLLETLMVTTKLSSFFTIC
                     PTLDAALDHFEKEKA"
     gene            complement(1371..1865)
                     /locus_tag="PLM90_13420"
     CDS             complement(1371..1865)
                     /locus_tag="PLM90_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMZ90431.1"
                     /translation="MEKEMQQTKYLEEIRNIFAAHLESKGLRKTPERFTILEEIYSRN
                     DHFDAESLYSHLLMKNYHVSRATVYNTLELLLSCDLVTKHQFGKNLAQYEKSYGYKQH
                     DHIICVDCKKVVEFCDPRIHQIQTMMGDLLNFKITHHSLNMYGVCGSCQKNRDRKSGE
                     TENK"
     gene            complement(1875..4082)
                     /locus_tag="PLM90_13425"
     CDS             complement(1875..4082)
                     /locus_tag="PLM90_13425"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HMZ90432.1"
                     /translation="METTSPIDLQKERKLILKEYRNLLRGLRNNMTAAERKQIRLAFE
                     MAVDAHKEMRRKSGEPYIIHPLRVAQIVVNEMGLGATAAICALLHDVVEDTELTLDDI
                     KREFGIKVMKIIDGLTKVSGVFDLTSSIQAENFKKLLLTLADDVRVILTKIADRLHNM
                     RTMDSMPRKKQLKIASETLYLYAPLAHRLGFYAIKSELEDLGMKYTESEAYKDIAKRL
                     AETKRERSRYMNEFMKPIRERLEKDGFHFEMYGRPKSIYSIYNKIKNKGVSFDEVYDL
                     FAIRVIIDTPFDHEKADCWRVYSTVTELYHPNPDRLRDWISSPKSNGYESLHTTVMGP
                     KGRWVEVQIRTKRMDEIAEKGFAAHWKYKEGSNDKALDTWLREIRELLRNPEFNALEF
                     MDDFRLNLFNKEIYVFTPKGELKIIPQGATALDFAFEIHTAVGYHCIGAKVNHRLVPL
                     SHRLSNGDQVEIITSKKQKPNEDWLNFLVTSRARSKVKNALKEEKRAIAAEGRIILEK
                     KLKSHKAELFDRNLQLLMEFYHQPTSLELCYRIGTKDIALKELKDFGVDQGKLIPPSK
                     DKQKPVDLAKLEKLATSNAELLIFDNALDDIKYSLAKCCNPIVGDDVFGFITINEGIK
                     IHKTSCPNATEMMSKYGYRIVRTRWTQHKDIAFLTGVKVTGLDDVGLVNKVTNIISGE
                     MRLNMQSLLMDAKDGVFEGTIKVFVKNTDELDKLMNKLRTMKGVISVTRLETT"
BASE COUNT         1201 a         1030 c          852 g         1417 t
ORIGIN      
        1 cgctgcattt gttcaataca accttcatgg atgccatttt ctttcatcac cttgtataac
       61 aatgagatat acaatggtat aaccggaatt gcagaacttg cttgtgtaac cagtgcttta
      121 tttacagaga caaatgcttt tccgtgtaca gactgcaatt tattcgtaat aacaccggca
      181 gtagattcca gatgatcttt agcgcgacca atagttcctt tgcggtatac aggttctgtt
      241 aatgacggac cgatatagct atacgcaatg gtcatcgcgt tatcagccaa tacatctgct
      301 ttcagtaatg catccatcca catttcccaa tcttcaccac ccattacagc tatagtattc
      361 tgaatatctt cttcattgca tggttcgatg cttatttcag aaacaatacc cgtatgaaaa
      421 tcaacagtct tatttttaaa agtgctgcca ataggtttca acaccgaacg atgtgttaca
      481 cctgttacag gatgtgtgcg aaccggagaa gcaaggctgt aaatcacaag atcaattttt
      541 ccgagatctg ccttgatggt atcgattgtt ttcgttttta tttcattgga gaatgcatca
      601 ccgttaatac tctttgcata taatccggct ttctcggctt cagcttgaaa cgccgcgctg
      661 ttataccaac ccggtgaagc agtctttcct tcctgaggtg ccttttcaaa aaatacaccg
      721 atagttgccg cccctgaccc aaaagcagcg gaaatacgcg atgccaatcc aaaaccggtg
      781 gatgcaccaa ttaccaatac ttttttagca cctgctatag cacctttcga acgtacataa
      841 gcaatctggt tgctgacatt ttttgcgcaa ccttccggat gtgctgtcaa acaaataaat
      901 ccacgcattc ttggttctat gatcatacta tttggtttta atgattgttt gggtaatgaa
      961 ttgcaaagtt aaacaattga catgacctga atttccggaa tccaattcaa gctttttctt
     1021 tctcgaaatg atcgagtgcc gcatccaatg tcgggcagat tgtaaaaaag gagctcaatt
     1081 ttgtagtaac cattaaagtt tcaagcagat tattcatggc acaaataact acttctccat
     1141 caagtcggcg cacttttgtg agtgttttta aaaagaagtt caatccgctg gagttcatat
     1201 attccaattc cttcaaatca aaaataatac gcacaaagtt ttcttcaatt aaatcttcca
     1261 gttgttcatt caatggtata gtttcatact cgccaatcat gcgacctttt aaatgaataa
     1321 caattacctg ccctgttttc ttttctactt taaattccat atattcaaat tcatttattt
     1381 tccgtttcac cggatttgcg atcgcggttt ttctgacaac tgccgcaaac gccgtacata
     1441 ttcaaactgt gatgtgttat cttaaaattt aaaaggtcac ccatcatggt ctgtatctga
     1501 tggatgcgcg gatcgcaaaa ttccacaacc tttttacagt cgacacaaat aatatgatcg
     1561 tgttgcttat atccatacga tttttcatac tgtgccagat tttttccgaa ctgatgtttg
     1621 gtcaccagat cgcaggaaag taatagttcc aatgtgttgt aaactgtagc cctgctcaca
     1681 tgatagtttt tcatcagcag atgcgaatac aagctttcag catcaaaatg atcgttgcga
     1741 ctgtatattt cctccaatat ggtaaatcgc tcaggtgttt tgcgcagacc ttttgattcg
     1801 agatgcgccg caaaaatgtt acgtatctct tcaaggtact ttgtttgttg catttctttt
     1861 tccattacaa ttctttaagt ggtttcaaga cgagttacgg aaataactcc tttcattgtg
     1921 cgcaatttat tcatgagttt atctagctca tccgtatttt ttacaaacac tttaatcgtt
     1981 ccctcaaaca caccatcctt ggcatccatg aggagacttt gcatattcaa acgcatctcg
     2041 ccactgatga tattagttac cttattcacc aaaccaacat cgtctaatcc cgtaactttt
     2101 acaccggtga gaaaggcaat atctttatgt tgcgtccacc tggtacgaac aatgcgatat
     2161 ccatacttac tcatcatttc agttgcattc ggacaactgg ttttatgaat tttgatacct
     2221 tcattgatcg tgataaatcc aaaaacatca tcgccaacaa taggattaca acatttcgca
     2281 agtgaatatt taatatcatc gagcgcattg tcgaatatca gtaactcggc attgcttgtt
     2341 gccagttttt caagcttcgc aaggtcgaca ggtttttgtt tgtctttcga tggaggaatg
     2401 agcttgccct ggtctacacc aaaatctttt aactccttta acgcaatatc ttttgtgccg
     2461 atgcgatagc ataattcgag gctggtaggc tgatgataaa attccatcag caattgtaaa
     2521 tttctgtcga acaattcagc cttgtgactt ttcagtttct tttccagaat tattcttccc
     2581 tctgcagcga tagcgcgttt ctcttctttg agggcatttt tcactttgct cctcgcgcga
     2641 ctggtaacca aaaaattcag ccagtcttcg ttcggttttt gttttttgga tgtgataatc
     2701 tctacctgat cgccattgct caagcgatga ctcagtggca ccagacgatg atttactttc
     2761 gcaccaatgc agtgatatcc aacagcggta tgaatctcaa aagcaaagtc gagtgcagta
     2821 gcaccttgag gaataatttt cagttcacct tttggcgtga acacataaat ttctttgttg
     2881 aataaattca acctgaaatc atccatgaat tccagtgcgt tgaattccgg attgcgcaac
     2941 aactcacgta tttctctcag ccaggtatcc agcgctttat cgttagaccc ttctttatat
     3001 ttccaatgcg ccgcaaatcc cttttcagca atttcatcca tgcgttttgt gcgtatttgc
     3061 acttccaccc acctaccttt cggtcccatc acggtagtat gcaaactttc gtaaccgtta
     3121 ctttttggcg atgaaatcca atcgcgcagc ctgtcgggat ttgggtgata taattccgta
     3181 actgtagaat acacgcgcca gcaatcagct ttttcgtgat cgaaaggcgt atctatgatt
     3241 acgcgaatag cgaagagatc atacacctca tcgaagctga cgcctttatt tttgatttta
     3301 ttgtaaatgg aataaatact tttcggacgg ccgtacattt cgaaatgaaa accatctttt
     3361 tccaggcgtt cgcgaatagg cttcatgaat tcattcatat atcgtgaacg ttcccttttt
     3421 gtttctgcca atcgcttggc tatgtctttg taagcttccg attcggtata tttcataccg
     3481 aggtcttcaa gctcactttt gatagcataa aatcctagtc gatgtgcgag tggcgcatat
     3541 agatataaag tttctgaggc aatcttcaac tgttttttgc gcggcatgga atccatggtg
     3601 cgcatattgt gcagacggtc tgcaattttg gtcaggatca cgcgcacatc atcggccagc
     3661 gtcaacagca gtttcttaaa attctcggct tgaatagagc tggtaaggtc aaacacgccc
     3721 gacaccttcg taagaccatc gatgatcttc attactttga taccgaattc gcgtttgata
     3781 tcatcaaggg taagttctgt atcctccacc acatcatgca acaaagcgca gattgctgca
     3841 gtagcgccaa ggcccatctc attcaccacg atctgtgcca cgcgaagcgg gtgtatgata
     3901 tacggctcac cactcttgcg gcgcatctcc ttatgcgcat ccacagccat ctcaaaggca
     3961 agcctgatct gcttccgctc cgccgcggtc atattattcc gcagtccgcg cagcaaattc
     4021 cgatactcct taagtatcag cttccgttct ttctgaaggt cgatagggct ggttgtctcc
     4081 atgtacagtc tagactacaa agctattaca taactcattt gcttgtaaat tagttttgca
     4141 cgcaaagaaa aggatgggga catggggtgc gaaggagtca gcgtaaatct ttaggactat
     4201 ccgcagaatt cctatagccc ctaggtggga ttcgagaacc cctcggggct ctactacgct
     4261 gcgttttacg aggatatgtc attacgcatc ctagatccat aaactgagat tttccacggg
     4321 tcatcggaca ggacttacaa gtgaatcaat cacaaatctt gggagtcccg tgtcagaaaa
     4381 atattcagag ggagaacgtt tggtgggtcg gcgcgtgctt tcatgcctaa cataccgggt
     4441 gctcgctgaa gcattttgag aatgatgtgg cctacattgc cggatggctc aagaaactga
//
LOCUS       DAOQOU010000219         4471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_12502_length_4471_cov_8.007473, whole genome shotgun sequence.
ACCESSION   DAOQOU010000219 DAOQOU010000000
VERSION     DAOQOU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12502_length_4471_cov_8.007473"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..658)
                     /locus_tag="PLM90_13430"
     CDS             complement(11..658)
                     /locus_tag="PLM90_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90433.1"
                     /translation="MKLFSLISAIIFCTSAVIAQTDGQISSCYLFVDLYNNSATGFSE
                     YRKDMPDTAAEFELDDILFTAYQDLELGTGMIYKTPHQTSTIFQNYYYTKWEFNVEGK
                     ALYPNVLDQPWEVNKVEIEPYFTTLCNWIHDNCVGQLQMSPIYDVSALPNSFGVDKLT
                     CYLYHPAITLPEGLTTEQSIQMLEQVPSIKINVYENWFPKGTYRMEYSVIGIQQE"
     gene            complement(668..1384)
                     /locus_tag="PLM90_13435"
     CDS             complement(668..1384)
                     /locus_tag="PLM90_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90434.1"
                     /translation="MRSITLLAIITFLGITPAKSQSYGYGGIGYNMSFLQSDGLDFVI
                     GRYNDTRAYLTETMENPRYFDGISIHFGGAQKAFLFDFGYTQQSCVVSASGVDQSGIE
                     QQRDVKDKWNTIDIGLGVSAGASDHFSLAAGVNFGLNSEKYLTRAETPDNIGTANFAQ
                     INKQFKFGISPFVQFTITGESAGLLIRPYYAWSPVKTDYYDLNAYINPYTYILDPINI
                     EGTLKGFGLSVLFILYEYQD"
     gene            complement(1473..2921)
                     /locus_tag="PLM90_13440"
     CDS             complement(1473..2921)
                     /locus_tag="PLM90_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ90435.1"
                     /translation="MLTQLRSICLIPAMLCLMASGYAQTQILHFGSNEYALTPEMQSA
                     LNKDLEAFKQAPNISITGHTDADGSDGYNLALGEKRATSVKAYLISMGVDARNIEIYS
                     SGESQPLGNNQTAEGKEANRRVEVVANWVANPEKNLIKPMLIDDKTSIQVPQALLPSP
                     IYKTIDPTKANDVPIGDKGTVLHIPANAFVDAQGNVVKDHVKLAFTEYKNAADMAFSE
                     ISMTYQNDGEEMRFNSAGMFSLYGTSNGKDITIRDNTEINVDYALATQQHDIAFYGLQ
                     NNDTWELVENIAEMNVDTLVFGLQGDSLVLLDSTRKHSDYGFRLRRFNGEKIEEEDII
                     LWDPKTSSQMIRGVDAGHQYPDIVRGLSVGDFGVYNCDQIYRIGKSITINPVFVDEHG
                     QKISDGNILSLIDLNYNGAFSFDPNAYITLDANGRNVLLLFTKSNKLYLISESEMKEQ
                     KLNNNVSHTFTMKNVTEQLNSTEKLSEYLGLN"
     gene            complement(3040..>4471)
                     /locus_tag="PLM90_13445"
     CDS             complement(3040..>4471)
                     /locus_tag="PLM90_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ90436.1"
                     /translation="FTPKPFCMQFTSRITSFLCISLIAISVCNAQSISLYFGVDDATL
                     TPASIKNIDEQMSKLKAAPGLRVIGYADADGVNDDNDVLSDARAAAITNYLTTKGISS
                     DVIVAFGNGETMAKQNNASAYDKQLDRRVDIEVLNTTELALAKKLSTFKTPDRFKVTP
                     IIITFDPTKPNDIPIGTRGTILHIPANSFVDASGKNVTSTVTISFSEYTTSGEMVFSG
                     IPMHYKTGTDNQRFSSSGMFKIEGTAAGAAIEMAPNASVTMDYAMVGNQNDVGFYTLD
                     EKGEWQLINNIGVDGKPIASNTSSKTGNKTQETKQVKADSATAMQFANAFNNNLSTVK
                     TERITGSTTRASVKASLTLSGFGYYNCDALAPMVARVNIGGNFLDDQGKPILRINNIT
                     VVDLKFNGAFYYNNPANIQLDSRSDNMIFVSNQANRIFYVSNADMKAMKISKSGNYDI
                     KMKEITKTHTNNDQLSALIQSNKMSK"
BASE COUNT         1249 a          881 c          901 g         1440 t
ORIGIN      
        1 tcctctttcc ttattcctgc tgaattccaa ttaccgaata ttccattctg tatgttcctt
       61 ttggaaacca attttcataa acgttaatct taatacttgg aacctgctca agcatttgaa
      121 tggattgctc ggtagttaac ccctccggta atgtaatggc aggatgatag agatagcaag
      181 tcagtttatc tacgccaaat gaattcggta gtgcagacac atcatagatt ggcgacattt
      241 gtaattgacc aacacaatta tcgtgaatcc aattgcataa agtagtaaaa tacggttcaa
      301 tttcaacttt atttacttcc catggttgat ctaatacatt tggataaagg gcttttcctt
      361 caacattaaa ttcccatttg gtataataat aattttgaaa tatagtagaa gtttgatgcg
      421 gtgttttgta aatcatgcct gtacctaatt caaggtcttg ataggctgta aaaagaatat
      481 catctaattc gaactccgct gccgtatcgg gcatatcttt tctatattca gaaaatccgg
      541 tagcagaatt gttgtaaaga tcaacaaaca aatagcatga actgatttga ccatcggttt
      601 gtgctataac agctgatgtg caaaatataa ttgcactgat aagcgaaaat aatttcatgg
      661 aaataattta atcttgatat tcgtacagaa taaataatac agataatcca aatcccttca
      721 aagttccctc gatgtttatg ggatctagta tataagtata aggattgata taagcattca
      781 gatcatagta gtcggttttc accggcgacc aggcatagta tggtcttatc aggaggccgg
      841 cggattcacc ggtaatcgta aattgtacaa aaggcgaaat gccaaatttg aattgtttat
      901 tgatttgcgc aaagttggct gtgccgatat tatcaggcgt ttctgctctg gtaagatatt
      961 tctcactatt tagtccgaag ttcacaccgg cggctagtga gaagtgatcg cttgctccgg
     1021 cgctcacacc caacccgatg tcaatggtat tccatttgtc cttaacatcg cgttgttgtt
     1081 ctatgcccga ctggtcgaca ccacttgcgc taaccacgca gctttgctgg gtataaccaa
     1141 agtcgaagag aaatgctttt tgggcaccgc caaagtgaat ggatattcca tcaaaatacc
     1201 ggggattctc catggtttcc gtgaggtaag cccgtgtatc gttgtatctg ccaatcacaa
     1261 aatcaagtcc gtcggattga agaaatgaca tgttatagcc gattccccca tagccataac
     1321 tctgagattt tgcaggggta atgccaagaa atgtaataat tgcaagaagt gtaatcgatc
     1381 gcatagcgtt gtttcggttg tgtgtcagcc cgaaaattac gcaattttga aatcgtcggc
     1441 aaaatgatat ttatcatgaa gaccggtgtc gattaattta gtccgaggta ttccgaaagt
     1501 ttttctgtgc tgttaagctg ttcagttaca tttttcatag taaaggtatg agagacattg
     1561 ttattcaatt tttgctcttt catttctgat tcagaaatca aatacaactt gtttgatttt
     1621 gtgaaaagga gcaacacgtt tcttccgttt gcatcgagcg tgatgtaggc attagggtca
     1681 aaactaaaag ccccattgta gttaaggtcg atcaatgata gtatgtttcc atcacttatc
     1741 ttctggccat gctcatcaac aaaaacagga tttattgtta tacttttgcc aattcgatat
     1801 atctgatcgc aattgtaaac tccgaaatct ccaacactta agccacgcac tatatcagga
     1861 tattggtgac cggcgtcaac gccacggatc atctgagagc ttgttttggg atcccatagt
     1921 atgatatctt cttcctctat tttctcgcca ttaaacctgc gtaacctgaa accgtagtca
     1981 ctatgcttgc gtgtagaatc caataatacc aatgaatcac cttgtaatcc aaatactaag
     2041 gtatctacat tcatttcggc gatattttcg accaactccc acgtatcatt attctgcaag
     2101 ccataaaatg caatatcatg ctgttgtgtt gcaagcgcat agtcgacatt aatttctgta
     2161 ttgtcgcgaa tggtaatatc ttttccattc gatgtaccgt ataacgaaaa catacccgca
     2221 ctgttgaaac gcatttcttc gccgtcattt tggtaagtca tggatatttc ggagaaagcc
     2281 atatcggcgg catttttata ttctgtaaag gcgagtttaa catgatcttt cacaacatta
     2341 ccctgagcat ctacaaaagc attagcagga atatgtaaaa cagtgccctt gtcgccaatc
     2401 ggaacatcat ttgctttggt agggtcgata gttttataaa tcggtgaagg caatagtgct
     2461 tgtggaactt gaattgaggt tttatcgtcg atcaacatcg gttttattaa atttttttca
     2521 gggtttgcca cccaattcgc aacgacttcc acgcgtctgt tggcctcttt gccttccgcc
     2581 gtttggttgt tgccaagcgg ctgtgattca ccggagctat atatttcaat gttgcgcgca
     2641 tctacaccca tggaaattaa atatgcttta acagaggttg ctcttttttc tcctaaagca
     2701 agattatagc cgtcagagcc atcggcatct gtatgtccgg taatggaaat attaggcgct
     2761 tgcttgaagg cttccaggtc tttattcaga gctgattgca tttccggcgt taatgcatat
     2821 tcgttgctgc cgaaatggag gatttgggtt tgagcatatc cggatgccat aaggcaaagc
     2881 atggccggga tcaaacaaat tgatcgtaac tgggtaagca ttataatgtg tttagtgttt
     2941 tatagatgca ggtgcggatg ttattccaca accgatatta catttttatc cggtttgcac
     3001 aacatgaaaa ctcgatagga taagacttta tgatagccac tattttgaca tcttattact
     3061 ttgaataaga gccgacagct gatcattatt cgtatgggtt ttggtgattt ctttcatttt
     3121 aatatcatag tttcccgact tgctgatttt catggccttc atatcggcat tactcacata
     3181 gaaaatgcga ttggcttgat ttgaaacgaa aatcatatta tcagatcggc tgtcgagctg
     3241 aatattggcg ggattgttat aataaaacgc accattaaat ttcagatcca ccacggtgat
     3301 attgttaata cggaggattg gttttccctg atcatcgagg aaattaccgc cgatatttac
     3361 ccgcgctacc ataggcgcca gggcatcgca attataataa ccgaaccctg ataaagtcaa
     3421 agatgctttt acgcttgctc gagttgtgga gccggtaata cgttcggttt ttaccgtgct
     3481 caaattattg ttgaaggcat tggcaaattg catggcagtg gctgagtctg cttttacttg
     3541 ttttgtctcc tgggttttat tgcctgtttt gcttgacgta ttagaagcaa taggcttgcc
     3601 atcaacaccg atattattaa tcaattgcca ttctcctttt tcatccaggg tataaaatcc
     3661 gacatcattt tgattgccca ccatcgcata atccattgtg acagatgcat tgggtgccat
     3721 ttctatagcc gcacctgctg ctgttccctc aattttaaac atacctgaac ttgagaaacg
     3781 ttgattgtct gttcctgttt tgtaatgcat gggaatgcca ctgaatacca tttctcctga
     3841 agtagtatat tcggaaaatg aaatggtcac agtgcttgta acatttttac ctgatgcatc
     3901 tacaaacgag ttagcaggta tatgcaaaat agttcctctg gtcccgatgg gaatatcatt
     3961 tggttttgtt gggtcgaagg tgatgatgat cggtgttact ttaaatctat ccggtgtttt
     4021 aaatgtgctt aattttttgg cgagtgccaa ttcggttgta ttaagtactt ctatatccac
     4081 tcttcgatca agttgtttgt catatgcgct ggcattgttt tgttttgcca tggtttctcc
     4141 attgccgaaa gcaacaatga catcagaaga aattcccttg gtagtcaaat aattcgtgat
     4201 agctgcagcg cgtgcatctg ataatacatc attgtcgtca ttaacaccat cggcatcagc
     4261 ataaccaatc acacgcaagc cgggagctgc ttttagttta gacatttgtt catcaatgtt
     4321 cttaatcgat gccggtgtta atgttgcatc gtcgacaccg aaatagagtg aaatactttg
     4381 tgcgttgcaa acagaaatgg caatcaaaga aatgcacaag aacgatgtaa tgcgtgaagt
     4441 aaactgcata caaaatggtt taggagtgaa a
//
LOCUS       DAOQOU010000220         4325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_13225_length_4325_cov_4.820141, whole genome shotgun sequence.
ACCESSION   DAOQOU010000220 DAOQOU010000000
VERSION     DAOQOU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13225_length_4325_cov_4.820141"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLM90_13450"
     CDS             complement(<1..929)
                     /locus_tag="PLM90_13450"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HMZ90437.1"
                     /translation="MNETVMYVIIGAAALLLGILGGRLIFAPSKNQEALAKKEAEEII
                     ALAKKDAELITQKSKTEADTYKKERSLELKDQMIQQKAEVEKERQHKVGKLEEQELRL
                     KQRESQLGQQQSQVSKKEAELNNQRDTLNKQLESVNAKKAELDKAYETHIRKLEEVAK
                     LSADQARNELMEALKDEARNKAMAHIKEIVEEAKLKANKEAKKIILQTIQRTAAEQAI
                     ENSVSVFNLDSDDQKGQIIGREGRNIRALEAATGVEFIVDDTPETIIISGFDPVRREV
                     ARLSLQRLVTDGRIHPARIEEVVAKTKKQMEEH"
     gene            complement(1009..1320)
                     /locus_tag="PLM90_13455"
     CDS             complement(1009..1320)
                     /locus_tag="PLM90_13455"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMZ90438.1"
                     /translation="MESEESKLLNIHVTICDRPYRLKVRPEEEETVRKAARTIAEKIK
                     ELQNQFAGSDKQDYLAMALLTAMVEQLGAHKQSISQASDDGLAVALDTLNQQLADALQ
                     Q"
     gene            complement(1409..1720)
                     /locus_tag="PLM90_13460"
     CDS             complement(1409..1720)
                     /locus_tag="PLM90_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90439.1"
                     /translation="MEQSDNSEKLQLLRSNVERLLRSFQAVQQENDILKQQIEAIRRG
                     SADKTKRIEALEQQAELQKTAQTIANIARNEDGDDKTEVRKRINDLIKEVDNCIAMLS
                     E"
     gene            complement(1743..4184)
                     /gene="pheT"
                     /locus_tag="PLM90_13465"
     CDS             complement(1743..4184)
                     /gene="pheT"
                     /locus_tag="PLM90_13465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMZ90440.1"
                     /translation="MKISWNWLNELLPLPMSADEAAVLLTDIGLEVEGVYPYESIRGG
                     MQGLIVGKVVTCEKHPDADKLKVTTVDVGGGELLTIVCGAPNVAAGQKVIVAVVGTTV
                     YPVQGEPFTIKKAKIRGQESNGMLCAEDEIGLGESHAGLLILPEDTPIGIPVASLFDI
                     YTDQIIEIGLTANHADANSHFGTARELRAACIIRKGLNIAIQDLSLAQISVNKSAPKI
                     QVEVKNTDACPRYSGALLHNIKVESSPKWLQHKLRAVGMRPINNVVDITNYILHLYGQ
                     PLHAFDADKIKGNKVIVQTQPAGTTFVTLEGKSRSLKSEDLMICDAEEGMCIAGVYGG
                     LHSGITNESSSIFLESAYFNPSFIRRTESAHGLKTDASARFAKGTDIEITIKALSHAI
                     ALLQEICGATIEGDIIDIYPQVMSPFAVDLRAARLQAISAIQIEDSIVENILGALNIT
                     IARKSTGVWHTLVPTYKNDVTREIDLIEEILRMYGFNNIPTPKTVRTPYLVSPKPDRE
                     KVKMDTIRHLVSQGSYEIATNAISRSKYNQQFLPQLMHLQPTLLNSLNAELDSMRITM
                     AFTGLEVIAYNLSHKQSDLNLFEFGRVYHKTADAYREPEVLAFYKTGNVFAESWRIQQ
                     RKTGFYDLKEQVEKLMLHMGVKASWKPVDETHPLRPLFEQAVTVSVGNEVIGICGGIH
                     NAVLQSFGIKQAVWFAEINWQPLLLAMASRKVIFKEINKYPEMRRDLALVLDADVEYQ
                     SIAAIARQEGKQILREVNLFDVFEGEQLAGRKSYAIGLTFNDSTRTLTDSEVDAVMQR
                     LISRYEKEFNAVIRK"
BASE COUNT         1088 a         1000 c          853 g         1384 t
ORIGIN      
        1 atatgttcct ccatctgctt tttcgtcttc gcaacaactt cctcgatacg agcagggtga
       61 atacgaccat cagttaccag acgctgtaaa ctcaatcgtg ccacttcgcg acgaacaggg
      121 tcaaatccgc tgataataat agtttcaggc gtatcgtcaa caataaattc tacaccggtt
      181 gcagcttcca gcgcacgaat atttcgacct tcgcgaccga tgatttgtcc tttttgatca
      241 tcgctgtcga ggttgaaaac tgaaacggaa ttttcaattg cctgctctgc agcagtgcgc
      301 tgtattgttt gaagaatgat ttttttagct tctttatttg ccttcaattt agcctcttca
      361 acaatttctt tgatatgcgc catcgcctta ttacgggctt cgtctttcag cgcttccatt
      421 aattcgttgc gcgcctggtc ggcagataat ttggctactt cttcgagttt tcggatatgc
      481 gtttcataag ctttgtcgag ctcagctttt tttgcattta ctgattctaa ctgcttattt
      541 aaagtgtcgc gttgattatt taattcagct tcttttttag aaacctgcga ttgctgttgt
      601 cctaattgcg actctctttg tttgagcctt aattcttgtt cttcgagttt accaaccttg
      661 tgttgtcttt ctttttcaac ctccgctttt tgctggatca tctgatcctt cagttctaaa
      721 gagcgttctt ttttataagt gtcggcttca gttttgcttt tctgagtgat cagttctgca
      781 tcttttttag cgagggcgat aatttcttca gcttcttttt ttgccagtgc ttcctgattt
      841 tttgagggtg cgaagatcaa gcgacctcct aaaatgccca gtagtagggc tgcggcacca
      901 ataatgacat acattactgt ttcgttcata gctatgattt tatgatgacg aacgtaggcc
      961 gtgaccagca aaatacatct aaaaaggagt tacaggtagg gagtatgatc actgctgcaa
     1021 agcatcagcg agttgttggt ttaaggtatc aagggcaacg gctaaaccat cgtccgaagc
     1081 ctgtgatatg ctttgtttat gtgctcccaa ttgctccacc attgctgtta acaacgccat
     1141 agccagataa tcctgcttgt cgctgcctgc aaattggttc tggagttcct ttattttttc
     1201 ggcaatggtt cgggccgcct ttcgcacagt ttcctcttct tccggccgga cctttaacct
     1261 gtaaggcctg tcgcaaatcg taacatgtat attcaacaac ttgctttcct cgctttccat
     1321 ctgattttgt tgcaaacggt ttagtccaat acggactaaa gttaagtaaa tcttatgata
     1381 agtgtcacta cagctaaata aggaaacctt attcgctcaa catggcaatg caattgtcca
     1441 cttctttgat cagatcattg atgcgcttgc gcacctcggt tttgtcgtca ccatcctcgt
     1501 ttcgggcaat gttggcaatg gtttgcgctg ttttttgaag ctctgcctgc tgttctaacg
     1561 cctctatccg cttggtttta tcggcactgc cacggcgaat agcctcgatc tgctgcttga
     1621 gaatgtcgtt ttcctgctga acagcctgaa aactgcgcaa cagccgctcg acatttgacc
     1681 tgagcaattg aagtttttct gaattgtcgc tctgctccat ttaaaagcta atctacaact
     1741 ttttacttgc gtatcaccgc attaaattct ttttcatagc gagatatcag cctttgcatt
     1801 acggcatcta cctctgaatc ggttagggtt cgtgtgctgt cattaaaagt cagtccaatc
     1861 gcatagctct ttctgcctgc taattgctct ccttcaaata catcgaaaag gtttacctcc
     1921 ctgagtatct gcttgccctc ctgacgtgct attgctgcaa tggactgata ttctacatca
     1981 gcatccaata ccaatgccag gtcgcgacgc atctcaggat atttattaat ttccttgaaa
     2041 atcactttcc tcgatgccat agcaagcaat aagggctgcc aatttatctc tgcaaaccat
     2101 accgcctgct taatgccgaa ggattgtaaa acagcattgt gtatacctcc gcatatacca
     2161 ataacttcat ttcccacaga aacagttact gcctgctcaa ataatggacg caacggatgt
     2221 gtttcgtcta caggtttcca gcttgctttc acacccatgt gtaacatcaa tttctcaacc
     2281 tgctccttta aatcatagaa accggttttg cgttgttgta tccgccagct ttcagcaaat
     2341 acatttccgg ttttgtaaaa tgccaatacc tccggctcgc gataagcatc ggcagttttg
     2401 tgatacacac gtccgaattc aaataaattt aaatcgcttt gcttgtgact gagattatag
     2461 gctattactt ccagtccggt gaatgccatg gtaatgcgca tgctatcgag ttcggcattc
     2521 aatgaattca acagggtagg ttgcaaatgc attaattgcg gaaggaattg ctgattgtat
     2581 ttcgaacgtg aaatggcatt cgttgcaatc tcatacgagc cctgagaaac gaggtgacga
     2641 atggtatcca ttttcacttt ttccctatca ggtttcggac tcaccaaata tggcgtgcga
     2701 acagtttttg gtgtggggat attattaaaa ccatacatcc tcaatatctc ttcgataaga
     2761 tcaatttctc gtgtaacatc atttttatat gtaggcacca atgtatgcca aacaccggtt
     2821 gatttacgcg cgatggtaat attcaatgca cctaaaatat tttcaacaat gctgtcttca
     2881 atttgtatgg cactgatagc ctgcaatcgt gctgcacgca gatcaacagc gaagggcgac
     2941 atgacctgag gatatatatc aatgatatca ccctcgattg ttgcaccaca tatttcctgc
     3001 aacaatgcaa ttgcatgcga taaggccttt attgtgattt caatatctgt accctttgca
     3061 aaacgtgccg aagcatcagt ttttaatccg tgtgctgatt ctgttctgcg aataaatgaa
     3121 ggattgaaat atgcgctttc taaaaatata gagctgcttt catttgtaat acctgaatgc
     3181 aatccaccgt atactccggc aatacacatg ccttcctcag catcgcaaat cataagatct
     3241 tcagacttca ggctgcgcga tttcccttcc agagttacaa atgttgttcc ggcaggctgt
     3301 gtttgtacta ttactttatt gccttttatt ttgtccgcat caaatgcatg taagggctga
     3361 ccatataaat gcaatatgta attggtgatg tcaactacat tattaatcgg acgcatgcca
     3421 acagcgcgca atttatgttg cagccatttg ggtgatgatt caactttaat attatgtaac
     3481 aatgcacctg agtagcgcgg acatgcatca gtatttttta cttcaacctg tattttcgga
     3541 gcgcttttgt tcactgaaat ttgagcaagg gacagatcct gaatggcaat attcaatccc
     3601 ttgcgaataa tgcaggcagc gcgcaattca cgagccgttc caaaatgact attggcatcg
     3661 gcatgatttg ctgtgagtcc gatttctata atttgatcgg tataaatatc gaataaagaa
     3721 gcaaccggaa ttccgatagg tgtatcctca ggcagtatca ataatccggc atgtgattca
     3781 cctaatccta tttcatcttc ggcacacaac atgccattgc tctcctggcc acgaattttc
     3841 gctttcttaa tggtaaatgg ttctccttga acaggataaa ccgtagttcc gactacggca
     3901 actatcacct tttgtccggc agcaacattt ggcgcaccgc acacgatggt gagcaattca
     3961 ccaccgccaa cgtctacagt agtgaccttc aatttgtcgg cgtcgggatg tttttcacag
     4021 gtaacaactt tgcctactat caatccctgc atgccaccgc gaatgctttc gtagggataa
     4081 acaccttcaa cttccagtcc gatatcagtc agcaaaacgg cagcttcatc tgcactcata
     4141 ggcagtggaa gcaactcatt cagccaattc catgaaattt tcatactctg caaagttaat
     4201 aaccctgcgc aattttggtg gttctgaggg attaaagggg tttggctttc gaactccatg
     4261 aggatatgga atcaggtatc aggtatcagg tatcaagtat caggtatcag gtatcaagta
     4321 tcagg
//
LOCUS       DAOQOU010000221         4305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_13319_length_4305_cov_6.396471, whole genome shotgun sequence.
ACCESSION   DAOQOU010000221 DAOQOU010000000
VERSION     DAOQOU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13319_length_4305_cov_6.396471"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLM90_13470"
     CDS             complement(<1..260)
                     /locus_tag="PLM90_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ90441.1"
                     /translation="MSQPAKGHPKGLYVLFFTEMWERFSYYGMRAIFLLYLTDALVYA
                     IDDASNIYGSYTGLVYLTPLLGGYISDKYWGNRRSIFTGGIL"
     gene            complement(346..2016)
                     /locus_tag="PLM90_13475"
     CDS             complement(346..2016)
                     /locus_tag="PLM90_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ90442.1"
                     /translation="MKKLFRTKSISSVMANSDVADDIDHGSGLHRALTVRDLTFFGIA
                     AIIGAGSFSSMGSACAAGGPAVILLFIICGIACGFTAMCYAEFASRIPVAGSAYTYAY
                     TSFGEIIAWIIGWALLMEYSIGNIYVAFAWSDYFENLLGVIGIHMPGWLTTNYRSAHA
                     EMGDIWNNAPTLGSLHIIFDLPALLINFLITWLVFVGVKESRNVSNAMVIVKLAIIFL
                     VICVGAFYVNPDNWTPINDAGVRSFMPEGFTGVMSGVAAVFFAYIGFDAVSTMAEEAK
                     NPQRDLPRGMMYSLIICTIIYIILSLVLTGMAYYKDLKVGDPLAAVFQMNGVGWMEAI
                     ISIAAVAAMTSVILVFQMGQPRIWMSMSRDGLLPKKFQEIHPKYKTPGFSTIVTGLVV
                     GIPILFTDETFVLDFTSIATLFAFVLVCGGVLLIPRREKEKGRFHMPYVDGRYIYPIV
                     IFAAILFLNLIPATRGYFKDLFQIDFTADTATYKISSLIFWSLCVVLSVFAYKRRWSL
                     IPLLGLTTCCYLLTGMTAKNWVWFFIWFGLGLIVYFAYSRKHSKLNVA"
     gene            2098..2787
                     /gene="trmB"
                     /locus_tag="PLM90_13480"
     CDS             2098..2787
                     /gene="trmB"
                     /locus_tag="PLM90_13480"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HMZ90443.1"
                     /translation="MRKSKLARFAELDTFTNVLQNFNILEPVVVNNKKEVVDPKGKWH
                     DMIFRNQNPIVLELACGKGEYTCNMALADKSKNFIGVDIKGNRMHRGAKRALLEHIDN
                     AFFLRTDINIIETFIGPNEISEIWITFPDPHLKTGKFLKRLTSPRFLEKYKNILQPDG
                     IIHLKTDDPTLYEYTLEVIKEEGHRLIYADDDIYTKPLVNPFLEIKTYYEGMHLAEGR
                     KIKYVAFRLKA"
     gene            complement(3130..3675)
                     /locus_tag="PLM90_13485"
     CDS             complement(3130..3675)
                     /locus_tag="PLM90_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90444.1"
                     /translation="MLQVPVKAGSITHLTDARFFAAYDVPYIGFNFDPLSPNYISPTQ
                     AMAIKGWLTGPQFVAEFDNQDEENIRNIIAFFQPDIVQFTASIIRENPQLLQITELPL
                     IIDLNDACIPADSNNILFYIVDKLNRSHAIPAEKCMINYRDEIPPPNPQTPIHLTGTP
                     EQLTGIKVYDAIGDWMEGVSG"
     gene            3711..4082
                     /locus_tag="PLM90_13490"
     CDS             3711..4082
                     /locus_tag="PLM90_13490"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HMZ90445.1"
                     /translation="MATDQAPFFDLVWQVVRLIPKGRVTSYGAIARYLGTGRSARMVG
                     WAMNASHHAKPKVPAHRVVNRNGLLSGKAHFDPPEKMEQLLEKEGIKVKNNQIIDFKK
                     HFWDPNTELEEPDLMARSGKD"
BASE COUNT         1337 a          973 c          905 g         1090 t
ORIGIN      
        1 ataaggatac caccggtaaa aatcgagcgg cgattacccc aatacttgtc agaaatatat
       61 ccccccaaaa gcggagtcag gtaaactaaa ccggtgtagc taccataaat attggaagca
      121 tcatcgattg cataaaccaa tgcatcggtt aaatataaca ggaagatcgc gcgcattcca
      181 taataactga aacgctccca catctccgtg aagaagagta catatagtcc cttcggatgg
      241 cctttagcag gttgtgacat acggtgttgt tttgtaatga atatttatga tgttaaaaat
      301 gaatagaagt aagtaaatac ttttaatgtg tttaattcga tttcgttatg ctacattcaa
      361 tttactatgc ttgcgactgt atgcaaaata tacaatcaat cccaggccga accatataaa
      421 gaaccacacc cagttttttg ctgtcatacc tgtaagcagg taacaacatg tggtgagtcc
      481 gagtaatggt atcagcgacc atctgcgttt gtatgcaaat acagagagca ctacacacaa
      541 actccagaat attaaggagc tgattttata ggtagccgta tctgccgtaa aatcgatttg
      601 gaaaagatct ttgaaatatc ctcttgtggc aggaattaaa ttcaaaaata aaatagcagc
      661 aaatatcaca attggataaa tatatctgcc atcaacataa ggcatgtgga atctgccttt
      721 ttctttttcc cttcggggga tgagaagcac accaccgcaa acaagtacaa aggcaaacaa
      781 ggtggcaata cttgtaaaat caagtacgaa ggtttcgtcg gtgaataaga taggaattcc
      841 aacaacaagt cctgttacga ttgtagagaa tccgggtgtt ttatatttgg gatgaatctc
      901 ttgaaatttc tttggcaata atccatcgcg actcatgctc atccagatac gtggctgtcc
      961 catttgaaat accagaataa cactagtcat ggcagcaaca gcagcgattg aaatgattgc
     1021 ttccatccat ccaacaccat tcatttgaaa tactgctgcc aacggatcgc ctactttaag
     1081 atctttataa taagccatac ctgtaagcac aagtgataga atgatataaa taatggtgca
     1141 aataatcaat gaatacatca ttccacgcgg cagatcacgt tgaggatttt tggcttcttc
     1201 tgccatcgta ctcaccgcat cgaaaccgat ataggcaaaa aatactgctg ccactccact
     1261 catcactcct gtaaatcctt caggcataaa acttctcaca ccggcatcat taatgggagt
     1321 ccagttatcc ggattcacat aaaaagcacc gacacaaatc accaggaata taatcgccag
     1381 cttaacgata accatggcat tgctgacatt gcgactttct ttaacaccca caaacaccag
     1441 ccaggtgatc aggaagttga tgagtagtgc agggaggtcg aaaatgatat gtaaagaccc
     1501 aagggtaggt gcattattcc agatatcccc catctcggca tgcgcagagc gataattggt
     1561 agtgagccat ccgggcatat ggataccgat tactccaagt aaattttcga aatagtcgct
     1621 ccatgcaaaa gctacatata tattaccaat ggaatattcc atcaacaaag cccaaccgat
     1681 gatccaggcg atgatttcgc caaaggaggt ataggcatag gtataagcac ttcctgctac
     1741 cggaattcta ctggcaaatt ccgcatagca catggcagta aacccgcagg ctatcccgca
     1801 aataatgaaa agcaggataa cagccggtcc tcctgccgca caagccgagc ccatactgct
     1861 gaaactaccg gctccaatga tggccgcgat accaaaaaat gtgagatcgc gaacggtcag
     1921 cgccctgtgt aagcccgagc catgatcgat gtcatcagcc acatcggaat tggccatgac
     1981 actgctgatg ctcttggttc gaaagagttt tttcattcaa tagcggttct tgaggtcgaa
     2041 aagtaaaggg attttgccga ataaacaagg cagaagccta ttgcttacct ttgcggcgtg
     2101 agaaaaagta agcttgccag gtttgccgaa ctggacactt ttaccaatgt gttgcagaat
     2161 ttcaatatcc tggaacccgt tgtggtaaac aataagaaag aggttgtaga ccctaaaggg
     2221 aagtggcacg acatgatatt tcgcaaccaa aatccgatag ttctagagtt ggcgtgcgga
     2281 aagggtgaat atacctgcaa catggcactt gccgataaat ccaaaaattt cattggtgtc
     2341 gatattaagg gcaaccgcat gcacagagga gccaaaagag ccctgttgga gcatatcgac
     2401 aatgcctttt ttctgagaac agatatcaat attattgaaa ccttcatcgg tccaaacgaa
     2461 atcagcgaaa tctggatcac ttttcccgat cctcatctga aaacaggaaa atttctgaag
     2521 cgcctgacca gtccgcgttt ccttgaaaaa tataaaaaca tccttcaacc cgacggcatc
     2581 atccacctca aaactgatga tcctacttta tacgaataca cgcttgaagt aattaaggaa
     2641 gaaggacatc gcctcatcta cgccgatgat gatatttata ctaagccact ggtaaatcca
     2701 ttcctcgaaa taaaaaccta ctatgaaggc atgcacctcg cagaagggag gaagatcaag
     2761 tatgtggcgt ttaggttgaa agcttaattg gtatcaggga gccggtatca ggtatcaggt
     2821 atcaagtatc aggtagcggg ttactttgcg gctttgcgtg aaaaaagact cacgattcac
     2881 gcttcacgac tcacgatact cacctactca cccactcacc cactcaccct attttaatcg
     2941 cggagttggc ggaggtgtca cgaccaggca agttcccaat tcacgagtca cgaatcacga
     3001 ttggtagtga gtagtgggac tagcccaata ctgcctgtaa cgtcgttaca gcaaatgcac
     3061 taaaactctg cgactctgcg actctgcgtg aaaaaaaact cacgattcac gcttcacgac
     3121 tcacgatact cacccactca ccccttccat ccagtcgcca attgcgtcat atacttttat
     3181 gcccgtaagt tgctcagggg ttccggtaag gtggatgggg gtttggggat ttgggggagg
     3241 aatttcatcg cgataattga tcatgcattt ttctgcagga atcgcatgag agcgattaag
     3301 tttatcgacg atataaaaca gaatattatt actatcggca ggaatacatg catcgttcaa
     3361 atcgataatt aatggcaatt ctgttatctg caataattgc ggattttcac gaattattga
     3421 tgctgtaaat tgaacaatat cgggctgaaa aaatgcgatg atattgcgaa tattttcttc
     3481 atcctgatta tcgaattcag caacaaactg cggaccggtt agccaacctt ttattgccat
     3541 tgcctgagtg ggagaaatat aatttggcga taaaggatcg aaattaaatc ctatgtatgg
     3601 cacgtcatat gccgcaaaaa atcgcgcgtc ggtcagatgt gtgatactgc ctgctttaac
     3661 cggaacttgt aacattgcca caaaattccg actttcaacc catatttttt atggcaactg
     3721 atcaagcacc ctttttcgac cttgtctggc aagtggtccg attgattccc aagggaagag
     3781 ttaccagtta cggcgccatt gcacgctacc taggaaccgg tcgcagcgcc cgtatggttg
     3841 gttgggccat gaatgcatca catcatgcaa agccaaaggt tcctgcgcat cgtgtggtaa
     3901 ataggaatgg attattgagc ggcaaagccc atttcgatcc acctgaaaaa atggaacagt
     3961 tattagagaa agaaggtatc aaggtgaaaa ataatcagat catcgatttc aaaaaacact
     4021 tctgggatcc gaacaccgag cttgaggagc cggatttgat ggcaagaagt ggtaaggatt
     4081 aagatctgag ctttaggtag tgagtagtgg gtagtgagac tagcctattg attactgtaa
     4141 ctttgttaca gtaaatgcat taaaactctg cgactctgcg tctctgcgtg aaacaactac
     4201 gattaccctt attaccacga tccccctgct ccgccgccgc cgaagccgcc accaccgaag
     4261 cctccaaagc cgccaccacc gaagcctcca aagccgccgc caaat
//
LOCUS       DAOQOU010000222         4274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_13476_length_4274_cov_4.968476, whole genome shotgun sequence.
ACCESSION   DAOQOU010000222 DAOQOU010000000
VERSION     DAOQOU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13476_length_4274_cov_4.968476"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLM90_13495"
     CDS             <1..918
                     /locus_tag="PLM90_13495"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ90446.1"
                     /translation="IIKTKTAMEGVILKGIGSDFNWDGFNKYIIDGHGIDLTKDEASR
                     GTLLSKTTAERLDYHVGDSVLVHIIDQKENGDYQQMFRKLRIDGIYNTGLSEFDQLFM
                     LVDIRHIQRLNNWNADQIGGYEVFIDDPDRLDELEHEVHKAADPFWDVQTIKEIIPSV
                     FDWLNLQKVNEWIILVLMFMVALINMVTSLLILILERTNMIGILKAMGANNTAIRRIF
                     LLNASNIIVRGMLWGNIIGIGLCFLQQQFGLIKLPEESYYVSVAPVQLDWDWILMLNL
                     GTFLGSIIFLIIPSLFVSSIRPIKAIRFN"
     gene            complement(1323..1499)
                     /locus_tag="PLM90_13500"
     CDS             complement(1323..1499)
                     /locus_tag="PLM90_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HMZ90447.1"
                     /translation="MSGGKLIEGEDYYINAEGLFVFTAAFLLKRGFCCGNGCAHCPYP
                     KSEENTYPVEEKKP"
     gene            complement(1521..2873)
                     /gene="purB"
                     /locus_tag="PLM90_13505"
     CDS             complement(1521..2873)
                     /gene="purB"
                     /locus_tag="PLM90_13505"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMZ90448.1"
                     /translation="MDERALMSISPIDGRYQNKVTELSNYFSEYALMKFRIYVEVEYF
                     IALTEMPIPQFKNFEADNRSRLRKIYKKFNLDDCKVIKSIEEKINHDVKAVEYYIKQE
                     FDALGLSEYKEFIHFGLTSQDINNTAIPISFRDALIDVYAPLLEELINTLSELAVQWT
                     KIPMLARTHGQPASPTMLGKEIRVFIERLGKQRNLFNTIPYSAKFGGATGNFNAHFVA
                     YPDIDWILFADEFVSSRLGLERQRYTTQIEHYDNLAAAFDGLKRINTILLDLSRDMWT
                     YISMDYFKQRINKNEVGSSAMPHKVNPIDFENAEGNFGMANAIFEHLSAKLPISRLQR
                     DLTDSTVLRNIGVPMAHTLIAMKSVLKGLKKLQVNEQRIHDDLDNNWAVIAEAIQTIL
                     RREGYPQPYEALKELTRNGQAITKDTLQSFINGLDVSDDVKQQLLGITPFNYTGIRFF
                     "
     gene            complement(2953..3942)
                     /locus_tag="PLM90_13510"
     CDS             complement(2953..3942)
                     /locus_tag="PLM90_13510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMZ90449.1"
                     /translation="MPLKILVLRFSSIGDIVLTTPVLRCIKEQLPDCELHYATKKAYV
                     SLLSANPYIDKIHAFEEKKLPALMDALDAEKFDFIADLHHNLRTFLITSHLGIPNRKF
                     NKLNWQKWLMVKWKINKLPQQHIVDRYLKTVEPLGVKNDGKGLDHFIPADAHVAISSL
                     PQEFQSGYIALVIGAMHATKKMPPVKLVELVDRCSLPVVLIGGPDDVAAADIIVQQAT
                     NKVFSACGKYPLQQSASLLQQSIKVYAHDTGMMHIAAALKKDVISIWGNTIPEFGMYP
                     YIGTAYTIAQQRADGKILEVATLSCRPCSKLGYQSCPKGHFRCMLDIDLSGVK"
     gene            3969..>4274
                     /gene="miaE"
                     /locus_tag="PLM90_13515"
     CDS             3969..>4274
                     /gene="miaE"
                     /locus_tag="PLM90_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HMZ90450.1"
                     /translation="MLGLKLPTDPRWVNLAEKSLEEILTDHAYCEQKAASSCISIIQL
                     FPEYERVVDEVSPVVTEEWAHFRLVLKELKDRGLKLGKQRSDRYVHELSKFQLKGGTR
                     "
BASE COUNT         1176 a          842 c          975 g         1281 t
ORIGIN      
        1 atcatcaaaa ctaaaactgc catggaagga gtgatattaa aaggcatagg cagcgatttt
       61 aattgggatg gttttaataa atatatcatc gacggtcatg gcatagacct cacaaaagat
      121 gaagcttcac gcggcacact gctctcgaag acaactgccg agcgtttgga ctaccatgtt
      181 ggtgatagtg tactagtaca tatcatcgat caaaaagaaa atggcgatta tcagcagatg
      241 tttcgcaagt tgcgtattga tggtatttat aatacgggat tatcggaatt cgatcaatta
      301 ttcatgttgg tagatattcg tcatatccaa cggttaaata attggaatgc cgatcagatc
      361 ggtggatacg aagtatttat tgatgatccg gatagacttg atgaacttga acacgaagtg
      421 cacaaagcgg cagacccttt ttgggatgtg caaaccatta aagaaattat tcccagtgtt
      481 tttgattggc tgaatctgca aaaagtaaat gagtggatta ttttggtgct catgtttatg
      541 gtagcgctca tcaatatggt cacctcatta ttgattttaa tccttgagcg tacaaacatg
      601 ataggcatac ttaaggcaat gggcgccaac aacacagcta tcagaagaat atttttatta
      661 aatgcttcga atattattgt gcgcgggatg ttgtggggta atattatcgg tatcgggctt
      721 tgttttttgc agcaacaatt cggacttatc aaattacctg aagagtctta ctacgtatcg
      781 gttgcaccgg tgcagctgga ttgggattgg atattgatgc tgaatctcgg cacatttctg
      841 ggatctatca tatttctcat tattccttcc ttgtttgtat cgagcatcag gccgattaaa
      901 gcgataagat ttaattgatg gtgagtggtg agtggtgagt tgtgaatcgt gagtcgtgat
      961 tcgtgattcg tgactcgtga atttttacct gatacctagt ctagatacta gatacttggt
     1021 tctagatact aatgtcgtga ctcgtgaatt gaaaacttgc ctggccgata gccgggtcca
     1081 gacacctccg caaactccgc caactccgcg attaaaatag ggtgagtgcc ctcaccccgg
     1141 tcctctccca gagggagagg gagtgaattt cagaagtcat gtaaattatt ttattgtaac
     1201 gaagttacag ggccaaccgg gctagtccca ctactcacta ccaatcgtga gtcgtgattc
     1261 gtgaattttt acttgatact tgatacctga tacctgatac ttgatacctg ataccaattt
     1321 tcctaaggtt ttttctcctc aaccggatat gtattttcct cagattttgg atatggacaa
     1381 tgtgcgcatc catttccgca acaaaatcct ctcttcaata aaaatgcagc ggtaaacacg
     1441 aagagacctt cggcattgat ataataatct tcaccttcaa taagttttcc gcccgacatg
     1501 gttgacagtt ggtagtggaa ttagaaaaag cgtatgcctg tataattgaa tggcgtgata
     1561 cccaataatt gttgtttcac atcatcactc acatcgagac cattaataaa tgattgtaag
     1621 gtatcttttg tgatggcttg tccgttgcgt gtgagttctt ttaatgcctc ataaggttgc
     1681 ggatatcctt cgcggcgaag aatggtttgt attgcttctg caatcaccgc ccagttatta
     1741 tccagatcgt cgtgaatgcg ttgctcgttt acttgtaatt tcttcaatcc tttcaacact
     1801 gatttcatgg caattaatgt atgtgccatt ggaactccga tattgcgcag cactgttgaa
     1861 tctgtaaggt cgcgctgtaa tcttgatatc ggcaatttag ctgaaaggtg ttcgaatatt
     1921 gcattcgcca taccgaagtt gccctcagca ttttcgaaat cgataggatt cactttgtgt
     1981 ggcattgccg atgagcctac ttcatttttg ttgatacgtt gtttgaagta atccatcgaa
     2041 atataagtcc acatatcgcg acttaaatcg agtaaaatcg tattgatgcg tttcaatccg
     2101 tcgaatgcag ctgccagatt atcgtaatgt tcaatttgcg tagtataccg ctggcgttca
     2161 agtccgagtc gtgatgaaac aaattcgtcg gcaaacaata tccagtcgat atcgggatag
     2221 gcaacaaaat gtgcattgaa atttcctgtt gcgccaccga attttgcgct gtaaggaata
     2281 gtattgaata aattgcgttg cttgcccaat cgctcgatga acacacgaat ttcttttccg
     2341 agcatggttg gtgatgcagg ctgaccatgt gttcgcgcga gcatgggaat tttagtccac
     2401 tgcacagcga gttcacttaa tgtattaatc aattcctcca gcaagggtgc gtatacatcg
     2461 atcaatgcat cgcggaagct gataggaatg gcagtattat taatgtcttg cgaggttaat
     2521 ccgaaatgaa tgaattcttt gtattcactc aatcccaatg catcaaattc ttgtttgata
     2581 taatattcca ctgctttaac atcgtgattg atcttttctt cgatagattt gatcaccttg
     2641 caatcgtcga gattgaattt tttgtagatt tttcttagtc gcgagcggtt atcggcttca
     2701 aaatttttga attgtggaat aggcatttcg gtgagggcaa taaagtattc cacctcgaca
     2761 taaatcctga atttcatcag ggcatattcc gagaaatagt tagacagttc cgtcacttta
     2821 ttttggtaac gaccatctat cggggagata ctcatcagcg cgcgctcatc catcttgcct
     2881 gcatttagac tgcaaaatta cataatatac gatgaaaaga gggtaaagtc cgcacagggg
     2941 ttgcattgca ggctatttca ccccggagag gtcgatgtcc agcatgcagc ggaaatggcc
     3001 tttggggcag gattggtagc ccagttttga gcaagggcgg caggaaaggg ttgcaacttc
     3061 gagtattttg ccgtcggcgc gttgttgggc gatggtatat gcagttccga tgtaaggata
     3121 catgccaaat tccggaatgg tatttcccca gatgctgatc acatcctttt tcaatgccgc
     3181 tgcaatatgc atcatgccgg tatcatgcgc atacactttt attgattgtt ggaggagtga
     3241 agcactttgt tgcagaggat atttaccgca agcggaaaag actttattag ttgcctgttg
     3301 tacgattata tctgcagcag ccacatcatc agggccacca atcaacacca caggcaatga
     3361 acagcgatct accagttcaa caagctttac gggaggcatt tttttggtag catgcatggc
     3421 acctatcacc agtgcgatat atcccgattg aaattcttgc ggtaaagaag aaatagcaac
     3481 atgcgcatcg gcgggaataa aatgatcaag tcctttgcca tcatttttca cacccagtgg
     3541 ttcaacagtt ttgagataac gatctacgat atgttgttgc ggcagtttat tgatcttcca
     3601 tttcaccatc agccatttct gccaattcag tttattgaat ttgcgatttg gaattccgag
     3661 gtgggaggtg atgagaaatg tgcgtaaatt atgatggagg tcggcaataa aatcgaattt
     3721 ttccgcatcc agcgcatcca tcagagcagg taattttttc tcttcgaagg cgtgaatttt
     3781 atcgatgtat ggatttgccg agagcagaga aacatatgcc ttttttgtag cgtagtgcag
     3841 ctcgcaatcg ggcaattgct ctttgataca tcgcagcacc ggagtggtga gcacaatatc
     3901 gccgatagaa ctaaaacgca agaccaaaat ctttagcggc atgtgtaaat ttgcagcaaa
     3961 gttgcgaaat gttaggatta aagttaccaa ccgacccgcg gtgggtcaac ctggcagaaa
     4021 agagcctgga agagatcttg accgaccatg cttattgtga acaaaaggca gcgtcgagtt
     4081 gcatcagcat catccaatta ttcccggaat atgagcgtgt ggttgatgag gtttcgcctg
     4141 tggtaaccga agaatgggca cactttcgat tagttctgaa agaattgaaa gatagaggat
     4201 taaagcttgg caagcagcga tcagaccgct atgtgcatga gctcagtaaa tttcagctga
     4261 agggcggcac cagg
//
LOCUS       DAOQOU010000223         4273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_13480_length_4273_cov_5.604789, whole genome shotgun sequence.
ACCESSION   DAOQOU010000223 DAOQOU010000000
VERSION     DAOQOU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13480_length_4273_cov_5.604789"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1754)
                     /locus_tag="PLM90_13520"
     CDS             complement(39..1754)
                     /locus_tag="PLM90_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ90451.1"
                     /translation="MAQPAIKTNYGALSTLVTVFFFWGFIASGNGVFIPFCKHYFHLD
                     QFQSQLIDFAFYFAYYIGALLLFFFSTAGNNDLVAKWGFKKSIIYGLLFSFLGSLAMI
                     AAVNLGGFAAILISLFIVALGFSLQQTAAQPFAISLGDPATGNTRITLGGGINSFGTT
                     IGPIIVGFALFGTAAVSDDMINNLGLGSVTFLYVGIGALFLLCAALFRMSKKVPDGKM
                     ESHIEHAHKALTTMMVITAVIFIIFPIVFASYNSADAVKVEEMKTALAAIPETDTAAI
                     ATANTAIEELNAPIEQNRMILLSLALISVAGILLFAKSRATKNPNGWGALQYPQLVLG
                     MLAIFTYVGVEVSIQSNLGELLAQKAFGGYKASETAPFISIYWGSLMIGRWVGSVAVF
                     SMSDARKKLMMIIVPIIAFGVIIGVNSIVGHDMRMFYAYIVCIAVQIAGFFLGKDKPA
                     RTLLIFSVLGITSMIVGLMTTGNTAIFAFLSGGLFCSIMWPNIFALSLTKLGKYTSQG
                     SSLLIMMILGGGIIPPIQGKIADIIGIHSSYFLPALCFTYLAWFAWRMRNLLGENETV
                     AAGGH"
     gene            complement(1824..2321)
                     /locus_tag="PLM90_13525"
     CDS             complement(1824..2321)
                     /locus_tag="PLM90_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90452.1"
                     /translation="MKRSLPFLLLPLMWVLAGCPVSVDYPLGYQDKDKIDNALIGTWA
                     AADTSAEVQEVKISKIDEFTLAVEVTKRGSMYVEETDFFKGWCTTINNQKFVYFQNAG
                     DNTQGYYSYNYWFEGKQLMTSDFALKVGGVDAVTSIEAYRQEVKASMEYEDFRGTVFA
                     YYKTR"
     gene            2979..3536
                     /locus_tag="PLM90_13530"
     CDS             2979..3536
                     /locus_tag="PLM90_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ90453.1"
                     /translation="MAYITLPEGVPGIIGPMMFRPETAKPMNELVEVLLRGPHSLSSG
                     ERELIAAFTSYRNKTLFCYRSHGASAAHHLNGNIGLVYQVLEDYHRADISDKLKSLLA
                     IAEKVQMDGKLVAQQDIDNAKQHGATELEIHDTVLIAAAFCMYNRYVDGLGTFQPAEI
                     EAYRETGRMLAEDGYISAIPEPQTV"
     gene            3618..3812
                     /locus_tag="PLM90_13535"
                     /pseudo
     CDS             3618..3812
                     /locus_tag="PLM90_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
BASE COUNT         1306 a          865 c          921 g         1181 t
ORIGIN      
        1 acgcagaaaa gacctcaaat atatttttga gatgagttct aatgtcctcc agccgcaacg
       61 gtttcgtttt ctcccaacag attgcgcatt ctccatgcaa accaggcgag atatgtgaaa
      121 cataatgccg gcaggaagta gctgctgtgg ataccgataa tatctgcaat tttaccttgt
      181 attggaggga tgataccgcc gccaagaatc atcatgatca ataatgaaga accttgagaa
      241 gtatattttc caagttttgt gagcgacaat gcaaaaatat tcggccacat gatactgcag
      301 aaaagtccgc cacttaagaa tgcaaatatt gctgtgttac ctgtagtcat caatccgaca
      361 atcattgaag tgatacccag cacactgaat attaataatg tgcgagcagg cttatctttt
      421 ccaaggaaga atccggcaat ttgaacagcg atacaaacga tataggcata aaacattctc
      481 atgtcgtgac caacaatgct atttacacca atgatgacac caaatgcgat gataggaact
      541 atgatcatca tcaatttttt tcgcgcatca gacatggaga aaacagcaac agatccgacc
      601 catctgccta tcattaagct accccaataa atggaaataa atggtgccgt ttcagatgct
      661 ttgtatccgc cgaaagcctt ctgtgccaat agttcaccga gattactctg gatggaaact
      721 tccacgccta cataagtaaa tatcgccagc atgcccagta ctaattgagg atattgcaat
      781 gcaccccaac cgtttggatt ttttgtagct cttgattttg cgaataacaa aataccggca
      841 acagaaatta aggccagtga taacaaaatc atcctgtttt gttctatcgg cgcattcaat
      901 tcttcaatag ctgtatttgc tgttgcgatg gcagcagtat cggtttcagg aattgctgcc
      961 aaagcagttt tcatttcctc tactttaacc gcatcagcac tattataact ggcgaaaaca
     1021 attgggaata tgatgaatat cacagcagtg atcaccatca tagttgtcaa tgccttgtgt
     1081 gcatgttcga tatgcgattc catttttccg tcaggcactt ttttcgacat cctgaataat
     1141 gcagcacata acagaaaaag agcaccgatg ccaacgtata agaatgtaac cgaaccaagt
     1201 ccgaggttat tgatcatatc atcgctcact gctgctgttc cgaagagcgc aaaaccaaca
     1261 atgataggtc ctatggttgt gccgaaggaa ttaataccac cgccgagggt gatacgcgta
     1321 tttccggttg ccggatcgcc tagagatatg gcgaaaggtt gggcagcagt ttgttgcaaa
     1381 gaaaatccca gtgccacaat aaataagctg atcaggatgg ctgcaaatcc acctaagttc
     1441 acagcagcta tcatggccag agatcccaga aaagaaaaca gcaatccgta aataatactc
     1501 tttttaaagc cccatttggc taccaggtcg ttatttccgg cagtgctaaa aaagaacaac
     1561 aacaatgcac caatgtagta agcaaaataa aacgcaaagt cgatcaactg actttgaaac
     1621 tgatcgaggt ggaaataatg cttacagaat ggaataaata cgccgttgcc ggatgcaata
     1681 aatccccaga aaaagaaaac ggtaacaagg gtgcttaagg caccataatt agtcttgatt
     1741 gcaggttgag ccattgatac gcctgtttgt ggcgcaacaa ataagcatat aattcttcaa
     1801 attgccaaag aagttattga caatcagcgt gttttataat aagcaaagac agttcccctg
     1861 aaatcttcat attccatgga ggcttttacc tcctggcgat aagcttcaat agatgtaacg
     1921 gcatcaacac cgccaacttt aagtgcgaaa tcggaggtca tgagttgctt gccttcaaac
     1981 caatagttat aagagtagta gccttgggta ttgtcgcctg cattttggaa atacacgaat
     2041 ttctgattgt tgatggtagt gcaccatcct ttaaagaagt ccgtttcctc aacatacatt
     2101 gatccgcgtt tggttacttc tactgcgagt gtgaactcgt caatttttga tattttaacc
     2161 tcctgcactt ctgctgaagt atcagctgct gcccaggttc cgatgagggc gttatcgatt
     2221 ttgtccttat cctgataccc taaagggtaa tccacagaca caggacagcc ggccaaaacc
     2281 cacatgaggg ggaggaggag aaaggggagt gagcgtttca tggagtaaaa ataataaaat
     2341 aggagagagg agagaggaga aaggagagag gggtgagtgg gtgagtgggt cagtgggtga
     2401 gtggtgaaat taaatgctct gccgaaggca gagcgctatg atcaatttca agggcctgcc
     2461 ctgaagtgac aaaatatgca gcgaagcgga atattttttc tacttcaggg tttgggtaga
     2521 gatgtcatta tagattaagc tttgtagttg ccccggaagt agacaatttt ctctgttgca
     2581 ttacgaaaat ttacaattcg gggtaggcta tttgttttat tcaaaacggt ctgcctacgg
     2641 tagacaactt cctgacgccc acagggttct tgcatttgtc tcacaactca atacccacta
     2701 ctcactacca atggtgagtg gtgaatggtg agtggtgagt gggtgagtgg gtgagtggtg
     2761 aatggtgagt cgtgaatttt acctgatact tgatacctga tacctgatac aaattctaga
     2821 tactaaaacc aatcatgcat ccggtgccga tatgtgattt attaactaaa cgaagtgtaa
     2881 attttgttaa agtagtttga aacaattaaa catagtgtaa atggtttggc agcatcaatg
     2941 gagttggaat attttcatcc gttcaaagaa aaacaactat ggcatacatt actctacccg
     3001 aaggcgtacc gggtattatc ggacctatga tgttccgtcc cgaaaccgcc aaaccaatga
     3061 atgaattagt ggaagtgctg ttgcgaggtc cacattcact ttcaagcggc gagcgcgaat
     3121 tgattgctgc ttttacttcg tatcgcaata agacattatt ttgttaccgt tcacatgggg
     3181 cctctgcggc gcatcatttg aatggtaata tcggattggt gtatcaggtg cttgaagatt
     3241 atcaccgtgc agatatttcc gataaattaa agtccttgtt agcgattgct gaaaaagtgc
     3301 aaatggatgg aaaacttgtt gcacagcagg atattgataa cgcaaaacaa catggtgcta
     3361 ctgaattaga aattcacgac acggtattga ttgctgctgc tttctgcatg tataacagat
     3421 atgttgatgg tcttggtact tttcaacctg cagaaatcga agcataccgg gaaacaggac
     3481 gcatgctggc ggaagatggc tatatcagtg ccatccctga accacaaacg gtataaacaa
     3541 tttttattac aggcagaggt taagccgatg tacgcgagta tgtcggcttt ttttattttt
     3601 ataccctgat agccctcatg aatactttat atgatatttt agaagtttca aagcatgcta
     3661 ctgccgacga gataaaaaag gcctaccgtc ggttagcaaa aaaatatcat ccggatgtaa
     3721 atcccggcaa taaatcggca gaagaaaaat tcaagcagtt ggcgaatgct tatgaggtat
     3781 tgtctgatcc tcgcaaacga gcatcctatg attatatcac atatcactac cagcatgcag
     3841 gacctatgca tggccgaccc aacagtcaat cgacttcgca acgcaccggc tcttcaacgt
     3901 atgatcgcgg tccggtcgac aacagcaata gaaatgatcg aagggtagcg aatttgatat
     3961 ttgttgtcgg actcctcatt tttaatctca ttatccaaaa agactggagt cctgataggg
     4021 attatcattt taaatcaata aatatagatt ctctgcttaa atacaattcc acgtccgatt
     4081 ctatatccga atctccatat tttccattac ttggtgatac tattacgata ttgaaagaga
     4141 gggatagtat ttgatttagg gcaaattaat ggaatcagct tggcttattg ttagattgtt
     4201 gtttggtccc cggaaacctt taactgaaca tagattgcgc caggcttgca ttaattctgt
     4261 ataattactt ccg
//
LOCUS       DAOQOU010000224         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_13503_length_4268_cov_5.900309, whole genome shotgun sequence.
ACCESSION   DAOQOU010000224 DAOQOU010000000
VERSION     DAOQOU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13503_length_4268_cov_5.900309"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..2849
                     /locus_tag="PLM90_13540"
     CDS             120..2849
                     /locus_tag="PLM90_13540"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HMZ90454.1"
                     /translation="MDTFSYIANAEPAYIESLYHSFRTDPSSIDGEWRKFFEGFDFAQ
                     ANFSSNGSASVSSESLTDEFRTYALILAYREKGHLVATTNPLRPRKDRHANLKTEDFG
                     FSKGDMSKKMASGSLVGMQNATLEAIIAHLEACYCNNLGFEYDYIIEPAEKKWFEQQI
                     ESRAGNFGFSAEKKRRILSKLNETVVFEQFLDKKYIGQKRFSLEGGESTIPALDAIIN
                     AGASAGVVEFVFAMAHRGRLNVLANILGKTYEQIFNEFEGNAIPDTTMGDGDVKYHLG
                     YSSQVTTLSGNTVHLKLTPNPSHLEAVNPVLQGFVRAKSDVLYNEDEDRILPICIHGD
                     AAIAGQGVVYEVLQMSKLNGYKVGGTIHFVINNQIGFTTDFDDARSSNYCTSYAATVQ
                     APVIHVNGDDAEAVVFACELAVAYRQEFNKDVFVDMVCYRKHGHNEGDDPKYTQPTLY
                     KIIDAKKNPREIYVQKLLASDAIDRELANAMEQEFWNVLQQRLDAVKQHPLTYQYQPP
                     EEAWRKLKKVIDVEKDFATSPETKVTHAQLQSVLDKLFTTPDGFVKLKKIEKLFETNK
                     KLIAEGRIDWALGELIAYGTILLDGRDVRMSGQDVRRGTFSHRHAVLREENTNAEYIR
                     LNHLAEKQGKFRIFNSLLSEFGVLGFEFGYSLASPDHLVIWEAQFGDFNNGAQTIIDQ
                     FIAASVSKWQRMSGLVMLLPHGYEGQGPEHSSARLERFLQLCAEGNIIVTNITTPANF
                     FHALRRQLAFDFRRPMVNMSPKSLLRHPMAQSQVKELTEGKFHEVIEDAIDPKHVKRV
                     VLCSGKLYYDLLDYRQKNNRNDVALVRIEQLYPFPETQLERALAAYSGAEVFWAQEEP
                     RNMGAWYFMQEMMGRRISEAITRKPSASPATGFAKIHAREQQEIVEQAFGTLN"
     gene            2862..4070
                     /gene="odhB"
                     /locus_tag="PLM90_13545"
     CDS             2862..4070
                     /gene="odhB"
                     /locus_tag="PLM90_13545"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HMZ90455.1"
                     /translation="MIISLKVPTIGESINEVTLSRWLVKDGDIVALDQSLCEFESDKA
                     TLEFPSEKSGKIKIIAQEGADLKIGDEIATLDTDFAVAAAVKTEAPVKEESPVAPQEV
                     KKELHVTPLAEKIAEENKVDVSKVPGTGTGGRITKDDVLRFIKEGASPKEIIAARKQN
                     SRNQTQEKVSKLRKTIAKRLVAVKNETAMLTTFNEVDMSAVMAVREKYKAAFEKKYGV
                     GLGFMSFFVRACCNALQEFPAVNAYFNEEQVTFNDFCDISIAVSTPKGLVVPVLRNAE
                     SMSMAEVELKIKELATKGREGTLALEEMQGGSFTITNGGVFGSLMSTPIINAPQSAIL
                     GMHKIQERPIAFNGQVVIRPMMYLALSYDHRIIDGKESVSFLVRVKEQLEKPEQLLFG
                     MDPVGLLLEM"
BASE COUNT         1285 a          840 c         1012 g         1131 t
ORIGIN      
        1 caggtatcaa gtatcaggta ttgacaaatt cactactcac cactgaccca ctcaccactc
       61 accattcact atctttgccc tccaaaccaa agcgcccgcg gctttgttaa taggcataga
      121 tggacacttt ctcttacatt gcgaacgccg aaccggcata tatagagtcg ctttaccata
      181 gttttcggac tgatcctagc agtatagatg gcgaatggcg caaatttttt gaaggatttg
      241 atttcgctca agctaatttt tcttcaaatg gctcagcgtc agtaagttct gaatcgctga
      301 cagacgagtt ccgcacctac gcgctcattc tcgcttatcg tgaaaagggg catctggtgg
      361 ccaccaccaa ccccctcagg ccgcgaaaag accgccatgc caacctcaaa acggaagatt
      421 ttgggttttc taaaggcgat atgagcaaaa aaatggcatc aggttccctg gtcgggatgc
      481 aaaatgccac cctggaagcc ataattgccc atttagaggc ttgctattgc aataacctgg
      541 gctttgagta tgattatatt atcgaaccgg ctgagaaaaa gtggttcgaa cagcagatag
      601 aatcccgtgc aggcaatttt ggcttctcgg cagaaaagaa acgccgcatc ctgtccaagc
      661 tgaatgaaac tgttgttttt gagcagtttc tggataaaaa atacatagga caaaagcgat
      721 tttccctcga aggtggtgaa agcaccattc cggcgcttga cgccattatc aatgcaggtg
      781 caagtgcagg cgttgtggag ttcgtatttg ccatggcaca ccgcggaaga ttaaatgtgt
      841 tggcaaatat tcttggaaag acatacgaac agattttcaa tgaatttgaa ggcaatgcca
      901 ttcctgatac caccatgggt gatggcgatg tgaaatatca cctcggttat tcatcgcagg
      961 ttactacctt gagtggaaac actgtgcatc ttaagctcac tccgaatcct tcgcatcttg
     1021 aagcagtaaa tcctgttttg cagggttttg tccgcgcaaa atcagatgta ttatataatg
     1081 aggatgaaga ccgtattctt ccgatctgca ttcatggtga tgcagctatc gccggacaag
     1141 gtgttgttta cgaggtgttg cagatgagta aattgaatgg atataaagtt ggtggcacca
     1201 ttcattttgt gatcaacaat cagatcggtt ttactaccga ttttgatgat gcgcgttcaa
     1261 gcaattattg caccagctat gcagctacag tgcaggcgcc tgttatacat gtgaatggcg
     1321 atgatgcaga agctgttgta tttgcatgcg aattggcagt ggcgtatcga caagaattca
     1381 ataaagatgt atttgtagat atggtgtgtt atcgcaagca tggtcacaat gaaggcgatg
     1441 atcctaaata cacgcaacca acattatata agatcatcga tgccaaaaaa aatccgcgcg
     1501 aaatttatgt gcagaaatta ttggcgagtg atgctatcga tcgcgaattg gcaaatgcca
     1561 tggaacagga attttggaat gtattgcaac agcgtctcga tgccgtaaag caacatccgc
     1621 ttacatatca atatcaacca ccggaagaag catggcgtaa actgaaaaag gtgattgatg
     1681 ttgaaaaaga tttcgctaca tcccccgaaa caaaagtgac acacgcacag ttgcaatctg
     1741 ttttagataa attattcact acgcctgatg gatttgtcaa attaaagaaa atagaaaagc
     1801 tgttcgagac aaataaaaaa ctcatcgcag aagggcgtat cgattgggcg ttgggcgaat
     1861 tgattgcata cggaactata ttgcttgatg gtagagatgt gcgcatgagc ggtcaggatg
     1921 tgcgcagagg aacattcagt caccgacatg ctgtattgcg cgaagaaaat accaatgcag
     1981 aatatattcg cttgaatcat ctggcagaaa aacaaggtaa attccgcatc ttcaattcct
     2041 tgctcagcga atttggagta ttgggatttg aattcggcta tagcctcgct tcacctgatc
     2101 atctcgtgat atgggaagcg caattcggcg atttcaataa tggggcacaa accatcatag
     2161 accaattcat tgcagcatct gtcagtaaat ggcaacgtat gagcggtttg gtgatgttgt
     2221 tgccacatgg ttatgaaggc cagggtcccg aacatagcag tgcgcgttta gaaagattct
     2281 tgcagctctg tgcagaagga aatatcatcg tgacgaatat taccacacct gcaaatttct
     2341 tccatgcatt gcgtcgtcag ttggcattcg atttccgcag gccaatggta aatatgagtc
     2401 cgaaaagttt gttacgtcac ccgatggcgc agtcgcaggt gaaggaatta accgaaggga
     2461 aattccatga ggtgattgaa gatgccatcg accctaagca tgtcaaacgc gttgtgttgt
     2521 gcagtggtaa attatattac gatctgctcg actatcgtca aaaaaataat cgcaatgatg
     2581 tggctttggt gcgcatcgag caattatatc ctttccctga aacacaactg gagcgcgctt
     2641 tagcagcata ttccggtgca gaggtgtttt gggcgcagga agaacctcga aatatgggcg
     2701 catggtattt tatgcaggaa atgatgggca ggcgtatttc ggaagctatc acacgcaaac
     2761 cgagtgcatc acctgcaaca ggatttgcaa aaattcatgc acgagagcaa caagaaatag
     2821 ttgaacaagc ttttggaaca cttaattaat cttttcagat catgattata tcattaaaag
     2881 taccaacaat cggagaatcc atcaatgaag ttacgctttc acgctggctg gtaaaggatg
     2941 gcgatattgt tgcgcttgat caatcgcttt gtgagttcga gagtgataaa gctacactcg
     3001 aatttccttc tgaaaaatca ggcaagatca agatcattgc tcaagaaggt gctgatctga
     3061 aaattggcga cgagatcgca acactggata ccgatttcgc tgtggctgcg gctgttaaaa
     3121 cggaagctcc tgtaaaggaa gaatcaccgg ttgctcctca ggaagtgaag aaagaattgc
     3181 atgtaactcc tttggcagaa aaaattgctg aagaaaataa ggttgatgtt tccaaagtac
     3241 cgggaacagg caccggaggc aggataacca aagacgacgt gttgcgtttc ataaaagaag
     3301 gtgcttcgcc gaaagagatc atcgcagcga ggaaacaaaa cagtcgcaac caaactcagg
     3361 aaaaagtaag caaactgcgc aagacgattg cgaaaagatt agttgctgtt aaaaatgaga
     3421 cagccatgct cactacattc aatgaagtgg atatgagtgc tgtaatggcc gtgcgagaaa
     3481 agtataaagc tgcattcgaa aagaaatatg gtgttggatt gggcttcatg agtttctttg
     3541 tgcgtgcctg ttgcaatgct ttgcaggagt ttccggcagt aaatgcgtat ttcaacgaag
     3601 agcaggtgac attcaacgat ttttgcgata tctctatagc tgtttcaacg ccaaaaggat
     3661 tagtagtgcc tgtattgcgc aatgccgaat ctatgagtat ggcagaggtg gaattaaaaa
     3721 taaaagaact cgccacaaaa ggacgtgaag gtacactcgc gttggaagaa atgcagggag
     3781 gttctttcac cattaccaat ggcggtgtat ttggatcatt gatgagtaca ccaattatca
     3841 atgctccgca aagtgctatt ttgggtatgc ataaaattca ggagcgaccc attgcattta
     3901 atggacaagt ggtcattcgt cccatgatgt atctcgccct cagctacgat caccgcatca
     3961 tcgatggcaa agaaagcgta agcttcttag ttcgtgtaaa agagcaactc gagaaaccgg
     4021 agcaattatt gtttgggatg gatccggtgg ggttgttgtt ggagatgtga ggtaatgaaa
     4081 tggatcagat tcaggtgtat aagttagaat tacttatttc tgagtatata tgaactattc
     4141 ctaatagcga aattcttaga aggtttgcat tatttgcgtt ccccaatatt ttcaagatgg
     4201 catttctaaa tggattaacg gttttccgtg aatatggatt acaatgagag aaaattaaca
     4261 ttaatctt
//
LOCUS       DAOQOU010000225         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_13586_length_4245_cov_6.104057, whole genome shotgun sequence.
ACCESSION   DAOQOU010000225 DAOQOU010000000
VERSION     DAOQOU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13586_length_4245_cov_6.104057"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..459
                     /locus_tag="PLM90_13550"
     CDS             208..459
                     /locus_tag="PLM90_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMZ90456.1"
                     /translation="MCCCYILHSITLDRYYIGHTCEDVQERLRKHLSDHEGFTGKAKD
                     WKIVYTEGFEDKSAAYHREQEVKGWKSRKRIAQLVQSSS"
     gene            590..2512
                     /locus_tag="PLM90_13555"
     CDS             590..2512
                     /locus_tag="PLM90_13555"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ90457.1"
                     /translation="MKKLIPMSKSLKCFVCMLFVIQATNGFAQTLPYQLNYFEGTHHM
                     TVGGNPPSDLYDINVIREINLLFYVDDWYDQLLDTHGTELAIPARLVYNGDTLPADVG
                     VYFRGHSSFFANDSIKKSVGITLDYIDSLQNIDGSKTLNLNCSFRDTTFMHEAIYENL
                     IQKYIPALSVNYVHLYLNDLDFGLYTNVQQLDKIYLKDWFITNDGSLWRAERTDGATD
                     ADPGGANGTGFSTLNYLGMDTSVYKNYYTLKETSISHPWEDIIDVCDPLENMPADVLE
                     DTINKYLDLDRTLWYMAAEILFTDEDSYVHKGGMDYFLYWEPETDRLVPLEYDGNSVM
                     DSMRYKWKIFYKGTDTNYALSYHFFAIPSIRQRYLAHFRTMFNEIYTEENLYGIIDDY
                     FELIDPYVATDPVKLVSYDQFLYGIDQLKETIANRRAYILDDNEFDNPSLNIENVKWI
                     SNGYNWGVVNSIQPVTISAQISGAPDVKQITLYYSTEIFGPFTKLIMYDDGLHGDQLA
                     GDLIFASAIPNFPVGTKVRFYVEATADDDVFTKSYNPEGAEHDVYYYEVKTDYIEVNN
                     FTNNYFTLLPNPASSFLYVHCGNPGNENLEIYSITGAKVFEAKLTVDMEINVATWSPG
                     LYVAKTTEFAYQIIVQ"
     gene            2516..>4245
                     /locus_tag="PLM90_13560"
     CDS             2516..>4245
                     /locus_tag="PLM90_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ90458.1"
                     /translation="MMKAHRLFLTFLSIVVCSLSMHAQAPEKPNIVFIILDDMNDYTG
                     TLGGQPQIKTPVMDSLFGLGTNFMNAYCNAPVCGPSRTSMLSGKDMKYTQVYDNNEYL
                     DEFRDNFTNELGNEEVITIPEYLKNSGQYYTCGINKIFHDPFNKDYDVDTEDPCLKTL
                     SWSKVISFSNWDSTTDKLEATNVGVDGYLWGVVDSAVEYELKDYRAVDSAIEFINDVA
                     SGEIALCDSVFFLALGFAMPHLDLYVPEGYFPADYLNDIYEEPFNYPYNFPENTYPFN
                     GIVMPPQPDPKWNDYYALGPLGHAISIGQKDVETSFSDYVDSLPMLPVIEPDLTDEDR
                     KEILMEAKRANAIMAYMAGVQFVDAQIGRLMDSIQLYPEIYNNTVFIIVGDNGFSFGE
                     KHHWLKRSLWETDIRVPFAIFGPSFPQHQVSYNAVSLLDLFPTICDIAGLPYPSFANG
                     EPYLDGKSIFPILNNASIAFAHPALISFEAENNKECSCFPQIAVRDDRFAYIRYQSDG
                     GDPVHDCILDSSVVEEELYEVGVHRETDPNEWNNLIDNPDYAPVVNYLQQFLPDSNLY
                     LETAYKVVIT"
BASE COUNT         1285 a          734 c          862 g         1364 t
ORIGIN      
        1 aatagtgtat acggagggat ttgaagataa gtctgctgct tatcggcgag aacgaaaaat
       61 taaaggttgg aaaagtaaga aacggatagc tcagttggtt cagagcatcc cgctttaagg
      121 cgggagggtc ctaggttcga gcccaagtgt tcccacaaca agaggtcgca ttgcgacctt
      181 tttttttgag ataaagtgtt gttgattatg tgttgctgct acattttaca ttctattaca
      241 ttagatcgtt attacattgg acatacttgt gaagatgttc aagaacgctt gcgtaagcac
      301 ttgtctgacc atgaagggtt caccggtaag gcaaaggatt ggaaaatagt gtatacggag
      361 ggatttgaag ataagtccgc tgcttatcat cgtgaacaag aggttaaagg ctggaagagt
      421 cgtaaacgga tagctcagtt ggttcagagc tcttcctaat aacccattca ctcaattacc
      481 catccagctc acaagctcta caggtcgctt cgctcaattc cggttgagaa tttaaaattg
      541 aatgataatt ttacattaag ttattattac cattcaatat ttacactttt tgaaaaagct
      601 cattcccatg tcgaaatccc tcaaatgttt tgtttgtatg ttgtttgtta ttcaagctac
      661 caacggcttc gcacaaacct taccgtatca attaaattat tttgaaggaa cccatcatat
      721 gaccgttggt ggtaatcctc cttcagattt atatgatatt aatgtgatac gtgagattaa
      781 tcttcttttt tatgttgatg actggtatga tcaattatta gacacccacg gcacagaact
      841 ggctattcct gccagattgg tttataacgg agataccttg cctgctgatg ttggagttta
      901 tttccgcggt cattcgagct tttttgcaaa tgattccatt aaaaaatcag ttggtattac
      961 attagattat atagattcac ttcagaatat tgatggttct aaaacactta atttgaattg
     1021 tagtttcaga gatactactt tcatgcacga ggctatttat gagaacctga tacaaaaata
     1081 catacctgct ttatcggtaa attatgtcca tctttattta aatgatttgg attttggact
     1141 ttacactaac gttcagcaac ttgataaaat ttatttaaaa gattggttta tcaccaatga
     1201 tggctcactc tggagggctg aacgcacaga tggagctaca gatgcagacc ccggaggtgc
     1261 taatggaaca ggattttcga ctttgaatta ccttggaatg gatacaagtg tctataaaaa
     1321 ttattatact ctgaaagaaa cgagtatatc ccatccatgg gaggatatta ttgatgtttg
     1381 cgacccactc gaaaatatgc ctgctgatgt tttggaagac actataaaca aatatctcga
     1441 ccttgaccga acactttggt atatggcagc ggaaatacta tttacagatg aagatagtta
     1501 tgtgcataaa gggggcatgg actatttttt gtattgggag cctgaaacag atcgtctcgt
     1561 gccgcttgaa tatgacggca acagtgtaat ggattctatg cgctacaaat ggaaaatatt
     1621 ttataaagga acagatacta attatgcctt aagctatcat ttctttgcca ttccttcaat
     1681 cagacaacga tatcttgccc attttagaac tatgttcaat gaaatttata ctgaagagaa
     1741 tttgtatgga attattgatg actattttga attaattgat ccgtacgtgg cgactgaccc
     1801 ggtcaaattg gttagctatg atcaattttt atatggtatt gaccaattga aagaaactat
     1861 tgcaaatcga agagcctata tcttagacga taatgaattt gacaatccat cattaaatat
     1921 tgagaatgta aagtggatca gcaatggcta taattggggc gttgtaaata gtattcaacc
     1981 cgtcacaata tcagctcaaa tatccggtgc gcctgatgtg aaacaaataa ctttatatta
     2041 ttcaacagag attttcggac catttaccaa attgattatg tatgatgatg gactccatgg
     2101 cgaccaacta gcgggggatt tgatttttgc atctgctatt cccaactttc ctgttggaac
     2161 aaaagtacgt ttttatgtgg aagcgacagc cgatgacgat gtctttacca aaagttacaa
     2221 cccggaaggt gcagagcatg atgtttatta ttatgaggtg aaaacagact atattgaagt
     2281 gaataatttt acaaacaatt atttcaccct tttaccaaat ccggcttcaa gctttcttta
     2341 tgtgcattgc ggcaatccgg gtaatgagaa tcttgaaatt tactcaatta caggcgcaaa
     2401 agtatttgaa gcgaaattga cggtagatat ggaaattaat gtagcaacct ggtcgccggg
     2461 gctatatgta gctaagacta cagaatttgc ttatcagata attgtacaat gacctatgat
     2521 gaaggcacac cgtttatttt tgacatttct ttctattgtt gtatgttcct tgagcatgca
     2581 tgcacaggcg cctgaaaaac ccaatatagt ttttatcatt ttagatgata tgaatgatta
     2641 taccggcacg cttggagggc agcctcagat taaaacgcct gttatggatt ccttgtttgg
     2701 cctgggaact aatttcatga atgcctattg taatgctcct gtatgtggtc cctctcggac
     2761 aagcatgttg agtggtaagg atatgaaata tacacaggta tatgataata acgaatacct
     2821 cgatgaattt agagataatt tcactaatga gctgggtaat gaagaagtta tcactatccc
     2881 tgaatattta aaaaattcgg gtcaatatta tacctgcggt attaataaaa tatttcatga
     2941 tccattcaat aaagattatg atgtggatac agaagatcca tgtttaaaaa cattatcatg
     3001 gagcaaggtg atctcgtttt ccaattggga tagtacaacc gataaactgg aagctacaaa
     3061 tgttggcgta gatggttacc tgtggggtgt tgttgacagc gcagtagaat atgaattaaa
     3121 ggattatcgt gctgttgact ctgcaataga atttattaat gatgtagcct caggggaaat
     3181 tgcattgtgc gattcagtat tttttctcgc attgggtttc gcaatgccac atctcgatct
     3241 atatgtacct gaaggttatt tcccagcaga ttatctgaat gacatttatg aagaaccatt
     3301 taattatcca tataattttc ctgagaatac atatcctttt aatggaattg taatgccgcc
     3361 tcagcctgat cctaagtgga atgattatta tgcgttgggt ccactcggac atgccatttc
     3421 cattggtcaa aaggatgttg aaacttcttt ttctgattat gtcgattcat tgcccatgtt
     3481 accggttata gagcctgatt tgacggatga ggacagaaag gaaattttaa tggaagcaaa
     3541 acgcgcaaat gcaattatgg cttacatggc cggcgttcag tttgtggatg cacagatagg
     3601 ccgattaatg gattcaattc aattatatcc ggaaatttat aataacactg ttttcataat
     3661 tgttggcgac aatggctttt cctttgggga aaaacaccat tggctaaaac gcagtttatg
     3721 ggaaacagat attcgtgtcc cttttgcaat attcggaccc tcttttcctc aacatcaggt
     3781 tagctacaat gctgtgagtt tgcttgattt attcccaaca atttgtgata tcgcaggtct
     3841 tccgtatcca tcatttgcaa atggtgaacc ttacctggat ggtaagagta tattccctat
     3901 tttaaacaat gcttcaattg catttgcaca tcctgctctt attagctttg aggcggaaaa
     3961 taataaagag tgctcctgct ttcctcaaat tgcagtgcga gatgatcgat ttgcatatat
     4021 ccgttatcag tctgatggcg gtgatccggt gcatgattgc attttagact caagtgtagt
     4081 tgaagaggaa ctatatgaag taggtgtgca tagagaaact gatcctaatg aatggaataa
     4141 tttaatcgat aatccagatt atgcccctgt agtaaactac ttgcaacaat ttttgcccga
     4201 tagtaatctt tacctggaga cagcatataa agttgttatc acaaa
//
LOCUS       DAOQOU010000226         4234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_13653_length_4234_cov_4.894472, whole genome shotgun sequence.
ACCESSION   DAOQOU010000226 DAOQOU010000000
VERSION     DAOQOU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13653_length_4234_cov_4.894472"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..267)
                     /locus_tag="PLM90_13565"
     CDS             complement(79..267)
                     /locus_tag="PLM90_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90459.1"
                     /translation="MQLITRFPLQLLAKAIAIVCGVSTAIGARRWFLQTIIFFTINSI
                     NNKHKKLRFAKSFPLPFS"
     gene            complement(385..828)
                     /locus_tag="PLM90_13570"
     CDS             complement(385..828)
                     /locus_tag="PLM90_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HMZ90460.1"
                     /translation="MYPSKLYYTCTAPSFTGKHKMAKKKQYTLEYIIKSSPLVLYNCL
                     TTATGLQQWFADLVDNDGNTFFFTWKNDTETAELISTEEDDYIRFRWDWQDDDEYFEF
                     RIDKTEITNDTILYITDFAEDREVDAQKRLWDAQIQNLIKRIGAG"
     gene            1052..2500
                     /locus_tag="PLM90_13575"
     CDS             1052..2500
                     /locus_tag="PLM90_13575"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMZ90461.1"
                     /translation="MSNNYELKLKEWNEKEMAAVEISTMATRLWFDKSVELIIFRRQL
                     VDARASEILNHHLYAQRFLNRPLTIEVTLLLTRALAKLDLAPSRIDMGRLGVEWLNEG
                     SNFADADAFVAGKMKDFIGKEKLVLKPKDVVLYGFGRIGRIAARVIINEAGKGEQLRL
                     RAIVTRSNSDEDILKRASLLRRDSIHGKFAGTIVEDLENKALIINGHTVHMIAADKPE
                     LIDYTAFGINDALVIDNTGIAREREKLSKHLEAKGVSQVLLTAPGKGDIPNIVHGINH
                     EKFDPSKEKIFSAASCTTNAVVPILYVMDKTFGIHHGHLETVHSYTNDQNLLDNYHKK
                     SRRGRSAPINMVITETGAASAAAKVLPELAGKFTGNAIRVPTPDVSLAIINLTLNKEV
                     TIDEVNNVLREAASFGTLVEQIDYSISNEIVSSDCIGNSHAVIVDSPATIVSPDKKSV
                     VLYCWYDNEFGYTKQVIRLSRYLAGVIRLRYY"
     assembly_gap    2533..2632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2823..4157
                     /gene="ffh"
                     /locus_tag="PLM90_13580"
     CDS             2823..4157
                     /gene="ffh"
                     /locus_tag="PLM90_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HMZ90462.1"
                     /translation="MFENLSDRLEGAFKSLKGQGKITEINIATTVKEIRRALVEADVN
                     YKIAKEFTDTVKDKALGQQVLTAVSPGQLMVKIVQDELTSLMGGDKADINIKGTPAII
                     LVAGLQGSGKTTFSAKLASHIKSKLGRNPLLVACDVYRPAAINQLQVLGEQIGVSVFA
                     DLESKDPVDIAKRAVSFAKMNNHNVVIVDTAGRLAVDEEMMDEITRVKAALQPQETLF
                     VVDSMTGQDAVNTAKAFNDRLDFDGVVLTKLDGDTRGGAALSIKYVVQKPIKFVSRGE
                     KMDTLDVFYPDRMAQRILGMGDIVSFVEKAQEQYDEEKAAELQRKIRKNQFDFEDFLS
                     QLRQIKKMGNMKDLLGMIPGVGKQIKDLDIDEKQFARLEAMILSMTPEERNNPDVMTG
                     TRRQRIAKGSGTTIQDVNQFMKQFEDMRKMMKMMNKMGGAKAARALGGMVKK"
BASE COUNT         1221 a          744 c         1008 g         1161 t
ORIGIN      
        1 tgtttgatgt attttagcat gtgcttttta tctgaccata tgatcataca ccccttgact
       61 tttggtgata cctttttgtc atgaaaaagg tagcggaaag ctttttgcga agcgaagttt
      121 tttatgcttg ttgttgatcg aattaatggt gaaaaaaata attgtttgta aaaaccacct
      181 cctagccccg attgcagtgg aaaccccgca gacaatggcg atagccttgg cgaggagttg
      241 caacggaaag cgggtgatca gttgcatgca gtggggaatt tgagtgctcc tgattattaa
      301 aatacttcag cctgcgattt gttgaaatat aaaatacggg taaaatgttt cctgtaaaaa
      361 ccggcgcggt aggtcaggct ttgatcaacc ggccccgatt cttttgatca gattttgaat
      421 ctgtgcatcc cacaaccttt tttgggcatc tacctcacga tcttcggcaa aatcggtgat
      481 gtagagaata gtgtcgttgg tgatctctgt tttatcgatg cgaaattcga agtattcatc
      541 atcatcttgc caatcccatc gaaagcggat atagtcatct tcttcagtag aaatgagctc
      601 tgccgtttca gtatcatttt tccaggtgaa aaagaaggta ttgccgtcat tgtcgaccaa
      661 atcggcaaac cactgctgca agccggtagc cgtagtcaaa caattataca gtaccagagg
      721 gctggacttt ataatatatt cgagcgtgta ttgtttcttt ttagccattt tgtgcttacc
      781 ggtaaaactt ggtgcggtgc aagtataata taatttcgaa ggatacaaaa ttttgtttta
      841 tgttttatct acattgagtg aacatcttga ctgattttag cggtggatta gggatttact
      901 gctaatttgc tgttttacaa gtgattgcaa gtggtttgga gcagctaaaa agcctaaaaa
      961 cgtgatatat cggctgtttt gacacccctc gtaagcccct ttttccttac ctttgcactt
     1021 caatttttca accttataac ccacaagata tatgagcaac aactatgaat tgaaactcaa
     1081 agaatggaac gagaaggaaa tggcagctgt ggaaatcagc acaatggcca cccgtttatg
     1141 gttcgacaag tctgttgagt tgatcatttt tcgccgtcag ttggtagatg cccgtgccag
     1201 cgagatcctc aatcaccacc tgtatgcaca gcgttttctg aaccgtccgc ttaccatcga
     1261 ggtgacgcta ttgttgaccc gagcgcttgc taagctcgat ttggcaccat cgcgcattga
     1321 tatgggccgt ttgggtgtgg aatggctgaa tgaaggcagc aattttgcgg atgcagatgc
     1381 ttttgtggca gggaaaatga aggattttat cggcaaagag aaattggtgc ttaagccgaa
     1441 agatgttgtg ttgtacggtt ttggccgaat tggtcgtatt gccgcccgcg ttatcattaa
     1501 cgaagccgga aagggtgaac agttgcgttt acgtgccatt gttacacgca gcaacagcga
     1561 tgaggatatc ctgaaacgtg caagcttact gcgccgcgat tctattcatg gcaaatttgc
     1621 gggcaccata gttgaagacc tcgaaaataa agcactcatc atcaatggtc atacagtgca
     1681 catgattgct gccgataagc ctgagttgat tgattatact gcttttggta tcaatgatgc
     1741 attggtgata gataataccg gtattgcccg cgagcgtgaa aaactgagca aacacctcga
     1801 agcgaaaggg gtaagtcagg tattgctgac agctcccgga aaaggtgata ttcctaatat
     1861 cgtgcatggc atcaaccacg agaaatttga tccttcaaaa gaaaaaatat tctctgcagc
     1921 aagctgtact accaatgccg ttgtgcctat cctgtatgtg atggacaaga cttttggtat
     1981 tcatcatggt cacctcgaga cagtgcattc atatacaaac gaccaaaacc tgctcgataa
     2041 ctatcataaa aaatctcgtc gcggacgttc tgcgccaatc aatatggtaa ttacagaaac
     2101 gggtgctgca agtgctgccg cgaaggtatt accggaactg gcagggaaat ttacaggcaa
     2161 tgccatccgc gttccaacac ctgatgtgtc acttgcgatc atcaacctta ctttaaataa
     2221 agaagtgacc attgatgaag taaacaatgt tttacgtgaa gcagcgtctt ttggtacact
     2281 ggtagagcaa attgattaca gcatcagcaa tgaaattgtg agcagcgatt gtatcggaaa
     2341 cagtcacgcg gtgattgttg attcacctgc aaccatcgtg tctcctgata agaaaagtgt
     2401 tgtgctgtat tgctggtatg ataatgagtt tggatataca aaacaggtaa ttcgcttgtc
     2461 gcgttatttg gccggggtta ttaggttgag gtattattga gttgaaggtg aggtagattt
     2521 gaagattcga agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgaagaa
     2641 tcgaagggtt gaagtaccaa gggtttgaag acacgagaaa tcgaagattc gaagtgcgaa
     2701 ttcgtttacg cattattatt tatcctgata cctgatactt gatacttgat accagatatc
     2761 agaaactata ttgagtatag aaagtcccat caataaattg atagttataa gtaaatacac
     2821 atatgtttga aaatttatcc gacaggttag aaggcgcctt taaatcgctg aagggccagg
     2881 gaaagattac ggagatcaat attgccacta cggtaaagga gatcagacgt gcattggtag
     2941 aggccgacgt gaattataaa attgcgaagg aatttactga tacggtgaag gataaagcgc
     3001 ttggtcaaca ggtgctcaca gcggtatctc ccggtcagtt gatggtgaag atcgtgcagg
     3061 atgaattgac atcattgatg ggtggcgata aagccgatat caatatcaag ggtactccag
     3121 ccatcatact ggtagccggt ttgcaaggtt cgggtaaaac tactttcagc gccaaattag
     3181 cttcgcatat taaatcgaag ttgggaagaa atccattgct tgttgcctgc gatgtttatc
     3241 gccctgcggc aattaatcag ttgcaggtat taggtgaaca aattggtgtg agtgtatttg
     3301 ccgatttaga aagcaaagat cctgtagata ttgccaaacg tgcggtttca tttgcgaaaa
     3361 tgaataacca taatgtggtg attgtggata ctgccggtcg acttgcagta gatgaggaga
     3421 tgatggatga gatcactcgt gtaaaagctg cgctgcaacc tcaggaaacg ttatttgttg
     3481 tggatagcat gaccggtcag gatgctgtaa atactgcaaa agcatttaat gacaggttgg
     3541 attttgatgg tgtagtacta acaaaattag atggtgatac cagaggtggt gctgccttat
     3601 caattaaata tgttgttcaa aaaccgataa aattcgttag ccgcggcgag aagatggata
     3661 cgctcgatgt attctatccc gatcgtatgg cgcagcgtat tttgggcatg ggtgatattg
     3721 tttctttcgt tgaaaaagca caggaacaat acgacgaaga aaaggctgcg gaattacaac
     3781 gcaagatccg caaaaatcaa ttcgactttg aagattttct ttcgcaatta cgccagatta
     3841 aaaaaatggg taacatgaaa gatttgctgg gtatgattcc gggagttggt aaacaaatta
     3901 aagatctgga cattgatgaa aaacaatttg cgagattgga agcaatgatt ttatctatga
     3961 cgccggaaga acgaaacaac ccggatgtga tgaccggaac tcgacgtcag cgtattgcaa
     4021 agggaagcgg aacaaccata caggatgtaa atcaatttat gaagcagttt gaagatatgc
     4081 gcaaaatgat gaagatgatg aataaaatgg gaggagcgaa agctgcaaga gcgctggggg
     4141 gaatggtgaa gaagtagagt ggaggaatga tgtgtggaat taattagcaa atttgagaat
     4201 tagcagatta gccaattggg gtgaattctt gcat
//
LOCUS       DAOQOU010000227         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_14027_length_4166_cov_5.769886, whole genome shotgun sequence.
ACCESSION   DAOQOU010000227 DAOQOU010000000
VERSION     DAOQOU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14027_length_4166_cov_5.769886"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="uppS"
                     /locus_tag="PLM90_13585"
     CDS             complement(<1..331)
                     /gene="uppS"
                     /locus_tag="PLM90_13585"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HMZ90463.1"
                     /translation="MSAGQPDPNNIPRHIAIIMDGNGRWAKLRDKARIYGHHAGVEAV
                     RKVVEACTELGVEYLTLYAFSTENWKRPEEEVNALMELLVRTIRRETPELDKNNVHIG
                     MIGDGHSL"
     gene            complement(349..1206)
                     /locus_tag="PLM90_13590"
     CDS             complement(349..1206)
                     /locus_tag="PLM90_13590"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ90464.1"
                     /translation="MRSLFIYVSVLAVIVTVPNRSSAQAAEIGLWGGLTHSFGDINNS
                     TESFQFLEPGAGVFFRYNLNSRIGYYLGLSGGQTRGYDSISNNIYQLRRNLSYRTTIY
                     DVSGRVDFNFLPLEREKPKYWFTPYVFMGLSVYYFNPQAYYNDEWVDLQPLGTEGQQF
                     PELTGNDKYQRVQISVPMGGGFKFALNKNITAGIEWNWHWLFTDYLDDVSTVYVDPSI
                     LASGSDGALAVALADRSTAIDIIPLGEPGKQRGDSEHRDKYLYTGIFLSYTIVDLKCD
                     PPGKWGAKF"
     gene            complement(1343..2194)
                     /locus_tag="PLM90_13595"
     CDS             complement(1343..2194)
                     /locus_tag="PLM90_13595"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ90465.1"
                     /translation="MKNTFALLVFIQITLSGFSQITNPDPYCSGTFDGGISGAMMKYI
                     SKVEIDEVSWQHVSGNANPSPGYTYFNNIEAPVVQQGNTYTFTVTHSGIAIHFVTVFI
                     DFNGNNNFDVTEGEAFFETANTPEFEFPTSPTTFSITIPNDALNGTTRMRVQVFEDDD
                     YTWNLFNNEPPPCTAYAAGQMLDYGETEDYDLVIGGETIAIQPGSIATDLIVSPNPAS
                     DYWSIQLASDAVYHIDLYSINVEKLISYTSSLPLSIPATDLPSGAYILHLSDDKGRFS
                     YVHLLKH"
     gene            complement(2267..3151)
                     /locus_tag="PLM90_13600"
     CDS             complement(2267..3151)
                     /locus_tag="PLM90_13600"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HMZ90466.1"
                     /translation="MHIAIYGRLIKKDALPYVQQMFNVLHERNIHVYIHESYFPHLVN
                     NIQFKSEPVLFNEKNMAVIARIDYIFSVGGDGTLLDTITIVRDTNIPIVGINIGRLGF
                     LANIGKDEIISLINALETDAVTRDVRTLVHLDSNRPDLFEGDAFALNEFTIHKKDSSA
                     MIVIHTYINGEFLMSYWADGLIVSTPTGSTGYSLSCGGPIIFPTSENFVITPVAPHNL
                     NIRPIVVSDSSVISFEIEGRSDQFLVTLDSRYESVDASVQLAVRKENFTVTLVRPHEQ
                     NFLDTLRNKLSLGLDQRN"
     gene            complement(3153..3818)
                     /locus_tag="PLM90_13605"
     CDS             complement(3153..3818)
                     /locus_tag="PLM90_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ90467.1"
                     /translation="MLAKDIISENIPPLKVKDTGTKAIEWMYEFKLSHLPLVDNKKYI
                     GLVSEEDILDFNNTNEQLGKFLRNLYKPFVRDTEHIYEVLRVAATLKSTVIPVIDAEE
                     TYLGLITLQSLLYHFAKMSSISEPGGVIILELNQKTDYVLSDIARMVESNDAHILSLY
                     FNVDPDTGKHAVTIKVDTTEVKHIVATFERYEYQVRAYFRESDLGDIMKDRYDSLMNY
                     LNI"
     gene            complement(3820..>4166)
                     /locus_tag="PLM90_13610"
     CDS             complement(3820..>4166)
                     /locus_tag="PLM90_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ90468.1"
                     /translation="KTEVTFYDTKHATKELVDELFDIVNDRNKVIRVLATAKSAIRHN
                     LAEEVSKITVPTLLIWGNNDTITPPFVGEEFHKLLPNSQLYFIDKCGHAPMMEQPEEF
                     NRYLDKFLKEIN"
BASE COUNT         1143 a          872 c          950 g         1201 t
ORIGIN      
        1 gtaatgaatg tccatcgcca atcatgccga tgtgcacatt atttttatcg agttcagggg
       61 tttcgcggcg tatggttctt acgagcagtt ccattagtgc attcacctct tcctccgggc
      121 gtttccagtt ttcggtgctg aaagcataaa gcgtcaaata ctcaacaccc agttctgtgc
      181 aagcttctac tacttttcgc actgcttcaa cgcctgcgtg gtgaccatag atcctcgcct
      241 tatcgcgcag ttttgcccag cgaccattac catccatgat aatggcaata tgacgtggga
      301 tgttgttagg atctggttga cctgcggaca taacgggctg caaagttatc aaaatttggc
      361 accccattta ccgggtggat cgcattttaa gtctacgata gtatatgaca gaaaaatgcc
      421 ggtatagagg tatttatcgc gatgttcgct atcgccgcgc tgtttcccgg gttcgccgag
      481 gggaatgatg tcaattgccg tgcttctatc ggccaaagcc actgccagtg caccatctga
      541 gccacttgcc agaatggaag ggtctacgta aacggtagaa acatcgtcca gataatcggt
      601 aaacagccag tgccagttcc attcgattcc ggcggtaata tttttattga gggcaaattt
      661 aaatccgccc cccattggta cactgatctg cacacgctgg tatttgtcgt tgcccgtgag
      721 ctccggaaac tgttgccctt ctgttccaag tggctgaaga tcaacccatt cgtcgttata
      781 ataggcttgc ggattgaagt aatacacaga aagtcccatg aaaacatagg gcgtaaacca
      841 gtatttaggc ttttcgcgct ccagggggag aaagttaaag tctaccctac ccgacacgtc
      901 ataaatggtt gtgcggtagc tcagattgcg tcgcaactga taaatattgt ttgaaatgct
      961 gtcgtagcca cgggtttggc cgccgctgag accgagataa tagccgattc tggagttcaa
     1021 attgtatctg aagaatacgc cggcacccgg ttccaggaat tgaaaacttt cggtagaatt
     1081 attgatatcg ccaaaagaat gcgtaagtcc gccccatagt ccaatttcag cagcctgtgc
     1141 cgaagaccgg tttggaacag taacaataac tgccagaacg cttacatata tgaataacga
     1201 ccgcatgaac ggaaccacaa agttagggca aatggtttta acgcttgcag gcgggaaata
     1261 ttgtacttcc tgctgaaggc agccaaatgt ccgctcaata ggtcagttgc gggatacaaa
     1321 gagccctgcg gcaaatcttc cctcagtgct taagcaggtg cacataacta aatcgtccct
     1381 tatcatcact aagatgcagg atataagcgc cggaaggcaa atctgttgcg ggaatggaaa
     1441 gtggtaggct tgatgtatag gaaatcagct tctcaacatt tatggagtaa aggtcgatat
     1501 ggtatacggc atctgatgcc aattgaatac tccaatagtc agatgccggg ttaggtgaaa
     1561 caattaaatc ggtggcaatg gatcccggtt gaatggcaat tgtttcacct ccaattacga
     1621 ggtcataatc ctcagtttca ccgtaatcta acatttgacc ggcggcatat gctgtgcatg
     1681 gcggaggttc attgttgaat aaattccagg tatagtcatc atcttcaaac acttgaacac
     1741 gcattcttgt tgtcccgtta agggcatcat tgggtatggt gatggaaaat gtagttggtg
     1801 aagtagggaa ttcaaattca ggggtatttg ctgtttcaaa aaatgcttcg ccttcagtaa
     1861 catcaaaatt attatttcca ttaaaatcaa taaataccgt cacaaagtga atggctattc
     1921 cggaatgggt gacagtaaaa gtataggtat ttccctgctg cacaaccggt gcttcgatat
     1981 tattaaaata ggtataaccc ggagaggggt ttgcattacc ggaaacatgt tgccagctta
     2041 cctcatcaat ttccaccttg gaaatatatt tcatcatggc gccgctgata ccaccatcaa
     2101 atgtaccgct gcaatacggg tcggggttgg tgatttgact aaaacccgaa agtgttattt
     2161 gtatgaaaac gaggagcgcg aaagtatttt tcatttcgca atgttattga caatgcgcaa
     2221 taataaatcg cgataaatgc ataattactt acaccaaaaa tctacttcaa ttgcgctgat
     2281 ctaaaccaag cgaaagttta ttgcgcaggg tgtcgaggaa gttctgttca tgaggtcgca
     2341 cgagggtaac ggtaaagttt tctttgcgta ccgccaattg cactgaagcg tcaacagatt
     2401 cgtagcgact atcgagggtg actaaaaatt gatcgctgcg accttcaatt tcgaagctga
     2461 taacagagct gtcgcttact acaatgggac gtatatttaa gttatgcgga gcaaccggtg
     2521 ttatcacaaa attctcggat gtcgggaaaa taatcgggcc tccgcagctc aatgaatatc
     2581 cggtagaacc tgttggtgtt gaaacgatca atccatcggc ccaatagctc atgaggaatt
     2641 cgccattgat gtaagtatgt atgacaatca tggcagagct atccttttta tgaatggtaa
     2701 attcattcaa tgcgaatgca tcaccttcaa agagatccgg tctgttggag tcgaggtgca
     2761 cgagtgttct cacatcgcga gtaacggcat ctgtttcgag tgcgttgatc aaagagataa
     2821 tttcgtcttt gccgatattg gcaagaaatc ccaatcgacc gatattaatt ccgacaatgg
     2881 gaatatttgt atcgcgaacg atggtgatag tatccagcag tgtgccatcg ccaccaacgc
     2941 taaagatgta atcgatgcgc gcgatgacgg ccatattttt ttcgttgaat aaaaccggtt
     3001 cgcttttgaa ttggatatta ttcaccagat gcggaaaata ggactcatgt atatatacat
     3061 gaatattgcg ttcatgcaac acattaaaca tctgttgcac ataaggcagc gcatcttttt
     3121 ttatcaggcg accgtagatg gctatgtgca tatcagatat tgagataatt cattaatgaa
     3181 tcgtagcggt ctttcatgat atcacccaga tcactttctc tgaaataggc acgcacctga
     3241 tattcgtaac gttcgaaagt agctacgata tgtttcactt cagtggtatc aaccttaatg
     3301 gttacggcat gttttccggt atccggatca acattaaaat ataagctcag aatatgggcg
     3361 tcgttgcttt ccaccatgcg ggcgatatcg ctgagcacat aatctgtttt ttgatttaat
     3421 tcgaggatga tcactccgcc gggttcactg atggaactca tttttgcgaa gtggtataac
     3481 aaactttgta aagtgattaa tccaagatag gtttcttcgg catcgataac aggaattact
     3541 gttgatttta acgttgccgc aacgcgaagc acttcataaa tatgttcggt atctctaaca
     3601 aagggtttgt aaagattgcg caggaatttt ccgagttgct cgtttgtatt gttaaaatcg
     3661 aggatatctt cttcgcttac gagaccgata tattttttat tgtcgacaag cgggaggtgt
     3721 gacaatttga attcgtacat ccattctatc gccttggtgc cggtgtcttt taccttcagg
     3781 ggcggtatat tctccgatat gatatctttt gcgagcaact taatttattt ccttaaggaa
     3841 cttatccaga taacgattaa attcctccgg ttgttccatc attggtgcat gtccgcactt
     3901 atctatgaaa taaagttgtg aattcggtaa aagtttatgg aactcctcac caacgaaagg
     3961 aggtgtaatg gtgtcgttat taccccagat gagcaaggta ggtacggtaa tttttgatac
     4021 ttcctcagcc aggttgtggc ggatggcgct tttcgctgtt gccagtaccc ggattacttt
     4081 attgcggtcg ttcacgatat caaaaagctc atcaaccagt tctttggtag cgtgttttgt
     4141 atcgtagaaa gtaacctctg ttttgc
//
LOCUS       DAOQOU010000228         4144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_14124_length_4144_cov_6.363903, whole genome shotgun sequence.
ACCESSION   DAOQOU010000228 DAOQOU010000000
VERSION     DAOQOU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14124_length_4144_cov_6.363903"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLM90_13615"
     CDS             <1..763
                     /locus_tag="PLM90_13615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ90469.1"
                     /translation="NTPDLSSAEAKALEITCQRAQLREGMNILELGCGWGSLTMFMAQ
                     KFPSAHVTGVSNSSTQKLHIESQCKMRGITNVRIITADMNVFDINETFDRVVSVEMFE
                     HMRNYQLLLEKISRFLKPDGKLFVHIFTHKEFTYFFDVVDDTDWMSKYFFTGGVMPSD
                     DLLFYFNDHLTIQDHWHWDGTHYGKTSEAWLSNMDAHKNEIMPILAETYGAEQSVKWW
                     VYWRIFFMACAELWNFNNGREWIVSHYLFTKKATV"
     gene            765..2024
                     /locus_tag="PLM90_13620"
     CDS             765..2024
                     /locus_tag="PLM90_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ90470.1"
                     /translation="MARIAIIGTGIAGMGAAWYLHRDHEITVFEADNRIGGHTNTVFV
                     EEPGQDIPIDTGFIVFNNHTYPRLTALFDALQVKVKKTDMSFAAQICDEHLEYCGSSL
                     NKLFAQRRRIFSPRYIRFLKQLNTFNKTCIEVFHNETLSALSIKDYLRMKKYSDDLLN
                     WYILPMSSALWSTPPDTTAQFPVLSLVRFFSNHGFLGLNTQFQWYTVSGGSEQYKQKL
                     IAPFKERIFVNQGIRSIRRTSDGVELTDVHGSVFQFDKVIVAAHGDQALKMWSDASQE
                     ERALLSPFSYQKNVATLHTDGSIMPKKKMVWSSWNYRTQTRGNNLVSSCTYWMNSLQD
                     VSDFTDYFVTINDPGIIDGNKVIKEIVYEHPVFTVEAMKAQAKLPELNRNGSLYFCGS
                     YFRYGFHEDALMSAANVCEQILGRKVI"
     gene            2061..2861
                     /locus_tag="PLM90_13625"
     CDS             2061..2861
                     /locus_tag="PLM90_13625"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HMZ90471.1"
                     /translation="MTPSTLYTCLVMHNRLSPKKHRFNYKVFMFFWDIDNSAEIAKSH
                     RLLSYNKGGVFSFRDRDHFKYPEGDNRNELTVRAKLNAWLKENGVVDMPEKVMLLTHI
                     RMFGYVFNPVSFYFCYNASGDCLYVVTEISNTFGEMKIFLIDQKQQQSFEQEDVKYFY
                     VSPFTDMDTSFRFKYQLPSDKLNLRIDVQDRSGDRFFISTLTGARRTLSDLRLLWYVV
                     RFPFITLQVIGAIHWHAFLLWLKKVPWHRKTEQASLQRDILNKSGQPD"
     gene            2957..4123
                     /locus_tag="PLM90_13630"
     CDS             2957..4123
                     /locus_tag="PLM90_13630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HMZ90472.1"
                     /translation="MQKGYMQLMLPEGEVLTFGDVGHHLHADAHIKSNNFFKRAFLFG
                     DIGFAESYMDGEWETSDLTKLLSWFLLNIDNAPTLSGSKMQSAALNIFRWYNKVFHSK
                     NENSLHGSKRNIAEHYDLSNDFFRLFLDPSMTYSSAYFTKPDLDLEAAQYEKYDSLCT
                     SLKLNPDDHVLEIGTGWGGFAEFAAKNYGCKITTITISKEQHAYAVERIQNAGLSQLV
                     EVRLQDYRHVEGTFDKVVSIEMIEAVGAQYLKTYFDKIQSVLKPSGVLAIQAIICPDA
                     RFDTLKKNVDFIQKHIFPGSLLPSIAAINASVNATSDMFLFGLKDLGKSYATTLAEWR
                     DRFNTNIDKIRQMGFDESFIRKWNYYFAYCEAAFDMRNINVVQMVYTRPNNYGF"
BASE COUNT         1229 a          753 c          917 g         1245 t
ORIGIN      
        1 taatacaccg gacctgtcat cagctgaagc aaaggcgctt gaaataactt gtcagcgagc
       61 tcagctgcgc gaagggatga acatacttga actgggttgt ggatggggaa gtctaaccat
      121 gtttatggca cagaaatttc cttcggcaca tgtaacaggt gtttccaatt cgtctaccca
      181 aaaattacat atagaatctc aatgtaaaat gcgcggaatt acgaatgtgc gcattattac
      241 tgccgatatg aatgtgttcg acatcaatga aacttttgat cgtgtggttt cagtggagat
      301 gtttgaacac atgcggaatt atcaattgct gcttgaaaaa atttcgcgat ttcttaagcc
      361 tgatggaaaa ttatttgtac atattttcac acacaaagaa tttacttatt tcttcgatgt
      421 ggttgatgat accgactgga tgagtaaata tttctttacg ggcggtgtta tgccttccga
      481 cgatctttta ttttatttta acgatcatct caccattcaa gatcactggc attgggatgg
      541 cacacattac ggaaaaactt ccgaagcgtg gttgtcgaat atggatgcac ataaaaatga
      601 aataatgccc attcttgcag aaacatatgg tgcggaacaa tcggtgaaat ggtgggtcta
      661 ttggcgcata ttttttatgg cctgcgccga actttggaat ttcaataatg gccgcgaatg
      721 gatagtgagc cattatcttt ttaccaaaaa agcgactgtt tgatatggcg cgcatagcaa
      781 taataggaac cggtattgcc ggaatgggcg ccgcttggta cttgcatcgc gatcacgaaa
      841 tcactgtatt tgaagctgat aacagaattg gtggtcatac caataccgtt tttgtggagg
      901 aaccgggtca ggacattccg attgataccg gttttatcgt gtttaataat catacttatc
      961 cgcgacttac tgccttgttc gatgcattgc aggtaaaagt gaaaaaaacg gatatgagct
     1021 ttgctgcgca gatttgcgat gagcatttgg agtattgcgg cagtagtctc aataaattgt
     1081 ttgcacagcg acgaagaatt ttttcgcctc gatatattcg ctttctcaaa cagttaaaca
     1141 ctttcaataa aacttgtata gaagtttttc ataatgaaac gctctcagct ttatcgataa
     1201 aggattatct gcgaatgaaa aaatattcag atgatctgct gaattggtat atcttaccta
     1261 tgagttcggc cttgtggagc actcctccgg atactactgc acaatttcct gtactaagcc
     1321 tggtgcgatt tttttctaac catggatttt tgggattgaa tacgcagttc caatggtaca
     1381 cggtttcagg aggtagtgag cagtataagc aaaaattaat agcgccattt aaggagcgta
     1441 tttttgtgaa tcagggtatc cgctcgatac gtagaacaag tgatggtgtc gaattgacag
     1501 atgtgcacgg aagtgtcttt cagttcgata aagtaatcgt tgctgcccat ggcgatcagg
     1561 cattgaaaat gtggagcgat gcatcacaag aggaaagggc attgttatcg ccattcagtt
     1621 atcagaaaaa tgtcgcaaca ctacatacag atggcagtat catgccaaag aaaaaaatgg
     1681 tgtggagctc atggaattac agaactcaaa cgaggggaaa taacttagtc tcttcttgca
     1741 cttattggat gaatagtctg caggatgtct ccgattttac tgattatttc gtcacgataa
     1801 atgaccctgg catcatagat ggaaataagg ttatcaaaga gatcgtttat gagcatcctg
     1861 tattcactgt cgaggctatg aaagcacagg cgaaactccc tgaattaaac cggaatggca
     1921 gtctttattt ctgtggaagt tattttcgct atggatttca tgaagatgcc ttgatgagtg
     1981 cggcaaatgt atgtgaacaa atattgggca ggaaagtgat ttaattataa tacaaactgc
     2041 tttcgtaaat ttatcaaata atgacacctt caacattata tacctgctta gtgatgcata
     2101 atcggctttc tccgaagaag cataggttta actataaggt atttatgttt ttttgggata
     2161 tagataacag tgcagaaatt gcaaagtcgc atcgcttatt atcctataat aaaggaggtg
     2221 tattttcatt ccgagatcgc gatcacttca agtatcctga aggggataac cggaatgagt
     2281 tgacggtcag agcaaaactg aatgcctggt tgaaagaaaa tggtgttgtt gatatgccgg
     2341 aaaaggtgat gttactcaca catattcgta tgtttggcta tgtattcaat ccggtgagtt
     2401 tttatttttg ttacaatgca tcaggcgatt gcttgtatgt tgtaacggag atcagcaata
     2461 cttttgggga gatgaaaata tttcttatcg atcaaaagca acaacaatca tttgagcagg
     2521 aagatgtgaa atatttttac gtttctccct tcaccgatat ggatacctcc ttccggttta
     2581 aatatcaact gccctctgat aagctgaatc tccgcatcga tgtacaagac agaagtggcg
     2641 atcgcttctt catcagcacg ctcaccggtg cccgtcgaac attatcagac ctccgattgt
     2701 tatggtatgt ggtcaggttt cccttcatta cccttcaggt gattggggct attcattggc
     2761 atgcgttttt gttatggctg aaaaaggttc catggcatcg gaaaaccgag caggcaagcc
     2821 tacaacgcga tattttaaac aagagtggac aacctgatta aaaataaatg cgtatatttt
     2881 cattattgtt tacccgaagc gagaactata tatgaacagt ttttataaat caaccatatt
     2941 acgaaacctg aaacccatgc aaaaagggta tatgcaactc atgttacccg agggtgaagt
     3001 gttgacattt ggtgatgtgg gacaccattt acatgcggat gcgcatatca aatcaaataa
     3061 tttcttcaag cgcgcatttt tgtttggcga tatcggtttt gcggagtctt atatggatgg
     3121 agaatgggaa acttctgatc ttactaagtt gctttcctgg tttttgttga atatcgataa
     3181 cgcaccaacg ctgtcaggaa gcaaaatgca atcagcggcg cttaatattt ttcgctggta
     3241 taacaaagta tttcattcta aaaatgaaaa ttctttgcat ggatcgaaac gaaatatagc
     3301 tgaacattat gatttgagta atgatttttt cagattgttt ctcgatccta gtatgaccta
     3361 ttcttctgca tatttcacca aacccgatct cgatctcgag gcagcgcaat atgagaagta
     3421 tgacagcttg tgcacttcat taaaattgaa tccggatgac catgtgctcg aaataggaac
     3481 agggtggggt ggcttcgccg aattcgcagc taaaaattat ggttgcaaga ttacaactat
     3541 caccatttct aaagaacaac acgcctatgc ggttgagcgc atacaaaacg caggattatc
     3601 gcagcttgta gaagtgcgat tgcaggatta ccggcatgta gaaggcacct tcgataaggt
     3661 ggtttctatt gaaatgattg aggctgttgg tgcgcaatat ttaaaaacct atttcgataa
     3721 aattcaatct gtgctgaaac caagtggtgt attggcaata caggccatta tttgtcccga
     3781 tgcacgtttt gacacgttaa aaaagaatgt cgactttatt caaaagcaca ttttccccgg
     3841 atcattatta ccgagcatag cagccataaa tgcatcagtt aatgctacca gcgatatgtt
     3901 tttattcgga ttaaaagacc ttggaaaaag ctacgccaca acattggcag agtggcgcga
     3961 ccggtttaat acgaatattg ataagatcag acaaatgggt tttgatgaat catttattcg
     4021 caaatggaat tactacttcg cctactgtga agctgccttc gatatgcgca atatcaacgt
     4081 tgtccaaatg gtctacacga ggccgaataa ttatgggttt tgagtggggg cagtgggtga
     4141 gtgg
//
LOCUS       DAOQOU010000229         4128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_14211_length_4128_cov_5.371471, whole genome shotgun sequence.
ACCESSION   DAOQOU010000229 DAOQOU010000000
VERSION     DAOQOU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14211_length_4128_cov_5.371471"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="PLM90_13635"
     CDS             complement(<1..1537)
                     /locus_tag="PLM90_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ90473.1"
                     /translation="MRSVLLSLILVSLLAACEMPENIQDGQTAYKLKKYKLAAELLQK
                     DYAKAELPDEKAKIAYQIGESYAYNNRYEQAADWYFKSSDLGYGSEATLQYALMLKAQ
                     EKYPEAMQYFNQYLIDEPFRRPEITIELNACQNAIDWMNRQQNEYERDTYVTRLNTLN
                     SGDADFDPVLFGNDKIIFTSSRTSSTGDNTDSWTGNKFYDLYVATRQGINNFSAPEPF
                     TGPFNSPYNDGSITFSRDGQEVFFTRCGSDDRKIDDYCGLYMSNYLGDNNWSDPIALP
                     FFEDTMNIGTPCLSPDGQTLFFAAVNPDGYGGADLYMSKRNAEGWDAAVNVGQSLNTK
                     GNEVFPSFDAAGTFYFSSDNLQGMGGLDIFSAQWKNGKFSNIQNLEYPINSGADDFGL
                     VMLPSTAYGKDTLAAGYFSSNRAGGLGNDDLYLFVKTKKKLPPPLYVLNGKVSRKVYE
                     IENDVNSKVIDTIPLDAATAQLFYPDQKKELAMFTVSESGMFTLFVDSTQQYKIVGSK
                     EGFF"
     gene            1764..3638
                     /locus_tag="PLM90_13640"
     CDS             1764..3638
                     /locus_tag="PLM90_13640"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HMZ90474.1"
                     /translation="MHFSLRNTLLAGSLAFAMPLFAQPKLDLQLVAGPAFDSPVDIAN
                     CGDSRLFIVEKSGYIRILNTDGTSSLFLDIDAKVKSSGSEQGLLGLAFDPNYAANGYF
                     YVNYTNNTATGNTTIARYTRNAVNPNIADVASEVILLNIVQPFSNHNGGNLEFGPDGY
                     LYIGTGDGGSSGDPGNRAQDITDQLLGKMLRIDVSTAPYSIPPSNPFVGITGDDEIWQ
                     YGLRNPWRFSFDRATGDLWIGDVGQNAWEEVDFQPAASTGGENWGWRCYEGNATYNTT
                     GCAGIGSYDFPIYVYNHSFATGGYAITGGYVYRGSDYPGMYGYYIFCDYVSGNWWTTV
                     SNGAGGWTSAKNDLTFGDISTFGESSDGEIYCANLNTGLIYKVIDLCSNFIGTTAVTD
                     ANYGFNNGAVNLTVSGGTSPYSYSWSNGATTEDISALAAGAYSVTITDANGCTATASA
                     TVNSVCGALPSITTTSVTSTTASFDWIDEGVASYKVQYRPSTGGAWTNVNTPVSNITI
                     TGLTPATSYKLKVSHTCPGGAKYSKNKNFMTPARLDGSEVVLPQIAPNPSSGVINISN
                     MTPQTEVMVFDLSGKQINAGIDVQAGTLDLNNVAEGLYMIRFMENGMLISSEKVLIAR
                     "
     gene            complement(3704..>4128)
                     /locus_tag="PLM90_13645"
     CDS             complement(3704..>4128)
                     /locus_tag="PLM90_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HMZ90475.1"
                     /translation="DQIHYANDIILNHDPKQIVIYCGENDFAASSSVTPDLVFDRFLQ
                     LYKIIRSKYPGVPVVCISMKPSPSKLEMMDKIYAYNALLQDYIAVDSSITYADIFTPM
                     LNPDGSPDRSYFFSDMLHMNKKGYQVWNSVVLPLIIKQ"
BASE COUNT         1217 a          815 c          859 g         1237 t
ORIGIN      
        1 taaagaatcc ttcttttgaa ccaacgattt tatattgttg agtgctgtcg acaaataatg
       61 tgaacatgcc gctttcactt acagtgaaca tagcaagttc ttttttctga tcaggataaa
      121 ataattgtgc tgtcgccgca tctaacggga tggtatcaat cactttactg tttacatcat
      181 tttctatttc atacactttt cgagaaacct tgccatttaa tacatacaat ggtggaggca
      241 attttttctt ggtttttacg aaaagatata aatcatcatt acctaatccg cctgcgcgat
      301 tcgacgagaa atatcctgct gccagcgtat ctttcccata ggcagttgaa ggcaacatca
      361 ccaaaccaaa atcatcggct cctgaattaa tagggtattc aagattttga atgtttgaga
      421 atttgccatt cttccattga gcagaaaaaa tatctaatcc tcccatcccc tgtaaattat
      481 cgctgctgaa atagaatgta cccgcagcat caaaggatgg aaatacttca ttgcctttgg
      541 tattcagtga ttgaccgaca tttacagcgg catcccatcc ctcggcattg cgtttgctca
      601 tataaagatc agcacctccg tagccatcgg gatttacggc cgcaaaaaaa agtgtctgtc
      661 cgtcaggtga taaacatggt gttcctatgt tcatagtatc ttcaaagaaa ggaagagcaa
      721 taggatcact ccaattgtta tcacccaaat aattgctcat gtacaatccg caataatcat
      781 ctattttgcg atcatcgcta ccacagcgag taaaaaatac ttcctgacca tcgcgactga
      841 aagtaattga accgtcgttg tatggagaat taaatggacc tgtaaagggt tcaggagctg
      901 aaaaattatt gattccctga cgcgtggcta catataaatc gtagaattta tttcctgtcc
      961 acgagtctgt gttatcaccc gttgaagaag tgcgcgatga agtaaaaata attttatcat
     1021 tgccaaacaa tacgggatcg aaatcggcat caccactatt caaggtgttt aatctcgtta
     1081 cataggtatc gcgttcatat tcattttgtt gcctgttcat ccaatcgatg gcattctggc
     1141 atgcatttaa ttctatcgtt atttcaggtc tgcgaaatgg ttcatctatc aggtattgat
     1201 taaaatactg catggcttca ggatattttt cctgagcctt aagcatcaat gcatattgca
     1261 acgttgcttc actgccataa ccgagatcac tcgatttgaa ataccagtcg gcggcttgct
     1321 cataacggtt attgtaggcg taactttcac ctatctgata cgcgatttta gccttttcat
     1381 cgggtagctc tgctttagca taatctttct gcaggagttc cgcagcaagc ttgtatttct
     1441 tcaatttata ggcagtttgt ccatcctgaa tattttcagg catttcgcaa gctgccagca
     1501 ggcttacaag gatcaaactg aggagaacgc ttctcatata gttatcaaaa gtataaaacg
     1561 ttaagggaaa gattttgttg tatgtcccat tgttttgtca tgattcatgg gttttaccgc
     1621 aaaaatcata cgaaatggag gttttatcga agaaaaaatg tgtaaaaaac tatctgttta
     1681 cacatatttt attattttag aactgcaaac gcctcctcgt ttgtcacaca ggcaatcatt
     1741 cattcactaa aaaagacatt tgtatgcatt tttcattacg aaacacccta ttggcgggca
     1801 gtctggcatt cgctatgcca ttatttgccc aacctaaact tgacctgcag ctggttgccg
     1861 gtcccgcgtt tgattcaccg gtcgatattg caaattgcgg cgatagcaga ttgtttattg
     1921 tcgaaaagtc gggctacatc agaatattga atactgatgg caccagctca ttatttctgg
     1981 atatcgatgc gaaagtaaaa agttcaggca gtgaacaagg attgcttgga cttgcctttg
     2041 acccgaatta tgcggcaaac ggatatttct atgtgaatta cacaaataat acagccacgg
     2101 gaaatacgac cattgcacga tacaccagaa atgctgtcaa tccgaatatt gctgatgtgg
     2161 caagcgaggt aattttattg aacattgttc agcccttctc aaaccacaat ggtggaaatc
     2221 tggaattcgg tcctgacgga tatttatata tcggaacagg cgatggtgga agcagtggcg
     2281 accccggaaa tcgcgcgcag gatattacag atcagttatt gggtaaaatg ttgcgcattg
     2341 atgtaagtac cgctccatat tccataccgc catcaaatcc tttcgttgga attaccggcg
     2401 atgacgaaat ttggcaatac ggactgcgca atccatggcg tttttctttc gatcgtgcaa
     2461 caggagatct ttggattggc gacgttggtc aaaatgcctg ggaggaagtt gattttcaac
     2521 ctgccgctag taccggagga gaaaactggg gatggcgttg ttatgaaggt aatgctacct
     2581 ataataccac cggatgcgca ggaatcggat catatgactt tcctatttat gtatataacc
     2641 actcatttgc tacaggcggt tatgctataa caggcggtta cgtttatcgt ggaagcgatt
     2701 atcccggcat gtatggatat tatattttct gcgattatgt ttcaggaaat tggtggacca
     2761 cagtttctaa cggtgcaggt ggatggactt cggcgaaaaa tgatttaaca ttcggcgata
     2821 tttctacatt cggagaaagt agtgatggtg aaatttattg tgctaattta aataccggtc
     2881 tcatttataa agtaattgat ctctgctcaa atttcatcgg cacaactgct gttaccgatg
     2941 cgaattatgg atttaataat ggtgcagtta atttaactgt atcaggaggc acatcgcctt
     3001 attcatatag ttggagtaat ggtgcaacaa ctgaagacat cagtgcgctg gctgcaggag
     3061 cttatagtgt tacaataact gacgcgaatg gatgtacggc aactgcatca gcaacggtaa
     3121 acagcgtatg tggagcatta ccttctatca ctacaacatc ggtaacaagt actacagcaa
     3181 gctttgattg gattgatgaa ggcgtagcat cgtataaagt gcagtatcgc ccttcaacag
     3241 gtggtgcgtg gactaatgta aatacaccgg tgagtaatat taccattacc ggtttaacac
     3301 ccgctacaag ctataaactg aaagtttctc atacttgtcc gggtggcgcg aaatacagca
     3361 agaacaagaa tttcatgaca cctgcaaggt tggatggcag tgaagtggta ttaccacaaa
     3421 ttgcacctaa cccaagcagc ggtgtcatta atatttctaa tatgacccca caaacagaag
     3481 tgatggtttt tgatttgtcg ggaaaacaaa ttaatgccgg aattgatgtt caggcgggaa
     3541 cattagacct taataatgtt gctgaaggat tatacatgat tcgtttcatg gaaaacggca
     3601 tgcttatttc gagtgaaaaa gtattgatag cacgttaatt gttatcgaac ataaaaaaaa
     3661 ggctgcgaag taattttccg cagccttttt ttattgcagt aaattattgt tttattatta
     3721 atggcaggac aacgctattc caaacttgat aacctttttt attcatatgc agcatgtcgc
     3781 tgaaaaaata acttctgtcg ggacttccat ccggattcag cataggtgtg aaaatatcgg
     3841 cataagtgat agaactgtca acggcaatgt agtcttgtaa taaggcattg taagcatata
     3901 tcttatccat catttccaat tttgaaggag acggtttcat cgaaatacaa acaaccggaa
     3961 cgccgggata tttggaacgt atgatcttgt atagttgcag gaacctgtca aacacgaggt
     4021 caggcgtaac acttgatgac gctgcaaaat cattctcccc gcaataaata acaatctgct
     4081 ttggatcatg gttgagaatg atgtcattgg cgtaatgaat ttgatcca
//
LOCUS       DAOQOU010000230         4062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_14613_length_4062_cov_7.159970, whole genome shotgun sequence.
ACCESSION   DAOQOU010000230 DAOQOU010000000
VERSION     DAOQOU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14613_length_4062_cov_7.159970"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1808
                     /locus_tag="PLM90_13650"
     CDS             369..1808
                     /locus_tag="PLM90_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HMZ90476.1"
                     /translation="MSSAVRNLEPKAIWNFFEDINAIPRPSKHEERISAYMVAFGEQL
                     GLRTVKDAVGNVIIFKPATTGMESKPTVILQSHLDMVHQKNSDTQFDFNTEGIRSRID
                     GDWVKAEGTTLGADNGMGVAAIMSLLASKDIPHPALEALFTIDEETGMTGAKELQKGL
                     LQGKILLNLDTEDDDEFSIGCAGGIDTNTSYDYTQEAPPTGAMAFRISVSGLKGGHSG
                     VDIHLERGNANKIMNRLMFDGDAFGLQIASIDGGSLRNAIPRESFATVVVNNAKYADE
                     VRARAAAICHEYHLQDEGLKIDIQSTEMPGMVMSFDDSRKLILAIQSAHNGVYTMSSA
                     ISGLVETSSSLARVIVKDGKFITQSLQRSSVESAKMDVAYTVAAPFRLMGCSVEHGGA
                     YPGWAPDPASKILHILEAQYVQLFGDKPKVMAIHAGLECGILGERYPGLDMISFGPTI
                     KHPHSPDEKVHIASVQKFWNLLLHTLANL"
     gene            1944..3275
                     /locus_tag="PLM90_13655"
     CDS             1944..3275
                     /locus_tag="PLM90_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90477.1"
                     /translation="MINRVAYLLLLFIPVFGFAQVPAGGGLIVNEINQGTGLKEFMEF
                     VVLGDPLNPCANVDLTGWVFDDNNGTFETCGTGVGIASGHYRFTSCYNAVPPGSILVV
                     YNVADPYVGMPPDDPTDADGDGVYIIPSNSTCLEANYSVPISIPADCNYSGAYSTPTL
                     TWAAGMANSGDVAQVRKPDYSFFHGFSYGDVNTVFPTWPSGADAGTSFNKGSGNLALD
                     CGSCWSSANYITTTAGAGTPGAANTVNNGYFIDNLINCTLNYDDLNDIGNCDLLLPIY
                     QVSLMADVDDHKVQLTWSYTGIIPDNYIIYSSRDGKLFDTIASGTVHEIAEETWTDNV
                     SASGAYYYQIIINDIYGETYHSKIVSANVDVVKQQLFPNPATTTFSLLQHPVHPYTSM
                     YLINMEGETLITAVVEDDASLIKIDISDLPPGHYLVCLTYEHGVDYFPVIH"
     gene            complement(3276..3539)
                     /locus_tag="PLM90_13660"
     CDS             complement(3276..3539)
                     /locus_tag="PLM90_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90478.1"
                     /translation="MNAYLIVYQTDQDISPLRKVLDTMRMVEHISDHSSIVLSFRSAE
                     DILDVIKPSNFEKIPIIIIKLDSVIYTDNIKADFFEPHSVEHL"
     gene            complement(3621..>4062)
                     /locus_tag="PLM90_13665"
     CDS             complement(3621..>4062)
                     /locus_tag="PLM90_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HMZ90479.1"
                     /translation="EGLDYNFDKVIVANSVNAHRLLQLAKRNHAGDQVKDALMRAYFI
                     EGRNIDDNKTLQAIGEAAGLPADSVEDLVNDEQQFRRQVFNEAAEAGHFGARGVPFFV
                     FNRKYAISGAQPVEAFSQVLEKVYNESPAIITETGDTCTSDGDC"
BASE COUNT         1144 a          832 c          883 g         1203 t
ORIGIN      
        1 tttcagtatc tagaatcaag tatctagtat ctagactttt gcgccttagc ctctttgcga
       61 ctttgcgcga acccgcctca ccccccgccc cacacctcct ttcgtcggag cggcaagctc
      121 tccatctcct accgtcgata gagagggagc ttattgcgct tcggaattaa attttattaa
      181 acaatatcat tgcattcact attaatgcgc cagcattaaa agcttccaca cttgctaacc
      241 tgctaacttc caaacttgct aactaaacga aaatctccat gcgggtgcat ttctacatca
      301 gctaagtggc taatcggcta attctcaaat taactaattc cccctacctt tgcatcaaaa
      361 tcaacaccat gtcatcagca gtccgcaacc tggaacccaa ggctatctgg aatttctttg
      421 aagatatcaa tgccatcccg cgcccttcta agcatgagga acgtatcagc gcttatatgg
      481 tcgcttttgg cgagcaattg ggattgcgca cggtgaaaga tgccgttgga aatgtgatca
      541 tctttaaacc ggcaacgaca ggaatggaat caaagcctac ggttattttg cagagtcacc
      601 ttgatatggt gcatcaaaaa aattctgata ctcaatttga ttttaatacc gaaggtatac
      661 gttctcgtat cgacggcgat tgggtgaagg ctgagggtac aactcttggt gccgataatg
      721 gtatgggtgt tgctgccatc atgagtttat tggcatcgaa agatattccg catccggcat
      781 tagaagcgtt gtttactatt gatgaagaaa ccggcatgac cggagcgaaa gaattgcaaa
      841 aaggtttatt acagggaaaa atattgttga atctggatac tgaagatgat gatgaatttt
      901 ctatcggttg tgcaggtggt atcgatacca atacttcata cgattataca caagaagcac
      961 ctccaacagg tgcaatggca tttcgcatta gtgtgagtgg attaaaaggc gggcacagcg
     1021 gcgttgatat tcatctcgaa cgcggaaatg caaacaagat catgaacaga ttaatgtttg
     1081 atggcgatgc ttttggatta caaattgcaa gtatcgatgg tggaagtttg cgcaatgcca
     1141 ttcctcgcga gtcatttgca actgttgttg taaataatgc aaaatatgct gatgaagtgc
     1201 gtgctcgtgc agcagcaatt tgccatgaat atcatttaca ggatgaagga ttgaaaattg
     1261 atattcaatc aactgaaatg ccgggcatgg tgatgtcgtt tgacgacagt cgcaaattaa
     1321 tactggcaat tcaatcggca cacaatggtg tgtataccat gagcagtgct atttccggat
     1381 tggtagaaac atcttcatca ctggctcgtg taattgtaaa agatggaaaa ttcattacac
     1441 agagtttaca gcgctcatct gttgaatcgg caaaaatgga tgttgcatat accgttgcag
     1501 ctcctttccg cttaatggga tgttctgtag agcatggagg cgcttatccc ggatgggcac
     1561 ctgatcctgc atcgaaaata ttacatatac tcgaagcaca atatgttcaa ctctttggcg
     1621 ataaacctaa agtgatggca attcatgccg gactggaatg cggtattttg ggagagcgat
     1681 atccgggact tgatatgatt tcattcggac ctaccattaa acatccgcat tcacctgatg
     1741 aaaaagtgca tattgcatct gttcaaaaat tttggaattt attgctgcat acacttgcta
     1801 atttataatt ggtgttatta aaacggtaat tgatttattt aattttataa tatcaataca
     1861 ggtttattaa catcgcgtaa ataagcaggt aacagactag tgttaccttt gtattctcca
     1921 acacaacgac acacacgaaa tttatgatca atcgtgtcgc atatctgtta ttgttgttca
     1981 ttccggtctt cggatttgca caagtgcctg cgggtggcgg cctgatcgta aatgaaatca
     2041 atcagggtac aggtcttaaa gaatttatgg aatttgtggt cctgggtgat ccgctgaatc
     2101 cttgtgctaa tgtggatctt acaggatggg tgtttgatga taataacggc actttcgaaa
     2161 cttgcggcac cggcgtagga attgcatccg gacattatcg ctttacttct tgctacaatg
     2221 cagtgcctcc gggatcgata ttggtggtat ataatgttgc cgacccatac gtgggaatgc
     2281 cgccggatga tcctacggat gcagatggcg atggtgtgta tattattcct tcaaacagta
     2341 cttgtcttga agcaaattat tcagttccga tttcaatacc cgccgattgt aattacagtg
     2401 gggcatactc tacaccaaca cttacgtggg cagcaggcat ggcaaacagc ggtgatgtag
     2461 cgcaggtgcg caaaccggat tattcatttt ttcacggatt ttcctacggc gatgtaaata
     2521 ccgtatttcc gacatggcct tccggtgctg atgcagggac aagctttaac aaaggaagcg
     2581 gcaacctcgc attggattgt ggcagttgct ggtcgagtgc aaattatatc accaccactg
     2641 ccggagcagg aactcccgga gctgcaaaca ctgttaataa tggatatttt atcgataatc
     2701 tgattaactg cactttaaat tatgacgact taaatgatat tggcaattgc gatttattat
     2761 tgccgattta ccaagtgtcg ctaatggctg atgtcgatga tcacaaggtg caattaacct
     2821 ggagttatac gggtatcata ccggataatt atatcattta cagcagtcgc gacggaaaat
     2881 tatttgatac tatagcctcc ggaactgtgc atgaaatcgc ggaggaaact tggacggata
     2941 acgtttctgc aagtggtgca tattattatc aaataatcat caatgatatt tatggcgaaa
     3001 catatcattc gaaaatagtt tctgcaaacg ttgatgtggt aaagcaacaa ttatttccta
     3061 atcccgctac cactactttc agtttattgc aacatcctgt tcatccatat acatcgatgt
     3121 atcttattaa tatggaagga gaaacactta ttacagctgt tgttgaagat gatgcgtcat
     3181 taattaaaat tgacatcagt gatcttcctc cgggacatta tttggtatgc ttaacctatg
     3241 agcacggagt tgattacttt cccgttatcc actaattaca aatgctccac tgaatgtggt
     3301 tcaaagaaat cggctttaat gttatctgta tagataacac tgtcgagttt tataatgata
     3361 ataggaattt tctcgaagtt ggaaggtttg atcacatcga gaatgtcttc ggcactcctg
     3421 aaactcagta cgatggagct gtgatcggaa atatgttcta ccatgcgcat agtatcaagc
     3481 acttttcgca atggactgat atcctggtca gtttgataaa caatcagata ggcattcata
     3541 atacttgttt tattaatgcc aaggtagcct atgttaccat gttgaacagt gacgaatgtt
     3601 acccttaatg gtattgtata tcaacaatca ccatcagaag tacaggtatc tcctgtttct
     3661 gtgataatgg ccggtgattc gttgtagact ttttccaaga cttgcgaaaa tgcttcaacc
     3721 ggttgggcgc ccgagattgc atatttgcga ttgaacacga aaaatggtac cccgcgagcc
     3781 ccaaaatgac ccgcttctgc ggcttcgttg aatacctggc gacgaaactg ttgttcgtca
     3841 ttcaccaggt cctccacact gtcggcgggg agtccggcag cttcgccaat agcttgcagg
     3901 gttttgttat catcaatatt tctgccttcg ataaaatatg cacgcatcag tgcatccttc
     3961 acctgatcac cggcgtgatt acgttttgca agctgtaaaa ggcgatgcgc atttacagag
     4021 ttggcaacaa tcactttgtc gaaattgtag tcaagtcctt ct
//
LOCUS       DAOQOU010000231         4058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_14641_length_4058_cov_4.978516, whole genome shotgun sequence.
ACCESSION   DAOQOU010000231 DAOQOU010000000
VERSION     DAOQOU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14641_length_4058_cov_4.978516"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLM90_13670"
     CDS             <1..480
                     /locus_tag="PLM90_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90480.1"
                     /translation="QAQQVPRFAKYPIGDSGCEGYFPGDPEFEMTWSDDSSKVYTGEV
                     ALDSFNYFAICVKLSEPQSASQREQLDLLESYMDFLQEALEVTGTAGYGEGHTLEYYP
                     SVSGVIDYWEDAGGNSYTVKGWTNTQYLVVLGIYGQGEYPYFNAGQMYLEGIRFPEE"
     gene            548..3943
                     /locus_tag="PLM90_13675"
     CDS             548..3943
                     /locus_tag="PLM90_13675"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ90481.1"
                     /translation="MKQLFTLLLAACCTYANGQLLINEIAPNNSGYYDDEDNTFPDWI
                     ELVNTGATTLNVGGYAITDDMDTWNQWLLGNYNIPAGGRLRIFASAKNRDCFTCGGVV
                     SEMHTNFKLSYGETLALYDDAGNLLDSLTIPTLQAGHTMARIPDGGTWCYSGTPTPNA
                     TNTGTCYSGYAAAPTFNTQPGFYTGSVDVTASGAEVRYTTDGDWPGFASTLYTTPIHV
                     TENTVVKLTGIEAGKLPSRVVTGSFFIDEPTLLPVVSISSNSCDMFDEGASCIAAYDG
                     ADGWEPDNPQISAAVEYFTADKVQQFEKRIKFETAGNSSIFVYPQRGMQFTCDEDFGE
                     TSEFQFNLFAASKPDLDSLNGFRVRANNDWGNSAARMKDVINNRIALPTNIVVPAYQN
                     VATFINGEYWGHYSAREELDAYFLRNNLGVNPDKVDLIRSGAGEDVWDIAEFGTMTAY
                     NELSTFFNTHNMADPDDYALALGLIDKENWIDHFALQFFVNNDEMAYNLRCFRSYDPD
                     MKWKFILWDTGAGSECETCNSIQSLINFPYLSDEINMMNDLMDNEDFKIDFINRYADL
                     MNYYFTWDRIESLIDVNADEIEAEIPAQNARWGTGTLATWNNGVSELKAFFDNRVYYQ
                     RNEIENYLDMNDQVNVTLEVSPAGAGYIKISTIIPQDLPWTGVYFDGAPVTVTVMANP
                     GYTFSNWTDNPFIASETDPSFTVNITENTTFTANFTGAAIANPIYISEINYNSDSSLQ
                     SGDWIEIHNTAGVAVDVSNYTFSNNYFYNKFLIPSGTIIPANGFLVLVEDTALFTSMH
                     PDVSSFVGPLHFPIRNEGDSVTLRNAVGEVLMSFAFNDKRPWPITADGYGRTLEFPYY
                     FTDPSLPENWLAGCIGGSPGLPYTPCIENPVIEEINYKSAITEDAGDWFEFYNWAASP
                     FDLSGWKVMDKNKNVFTIPNGTQIPGGGGYYVVYQDETKFFAQFPTVTNATGPTIFGF
                     DGEGDVISIFDQDGQLRYSIGYDDEAPYPLSPDGGGTALQMINTALNGNDPANWTESC
                     PEGSPGTEFVMPCANSIEQQEPELSLQVSPNPGSTLFNISIPGMTGEASILIADVTGN
                     IIYNGELIHTNEFIIDLTKYPAGIYHVRAVNGDYTYTAQIVKL"
BASE COUNT         1167 a          839 c          909 g         1143 t
ORIGIN      
        1 caagcacagc aagttccaag atttgccaaa tatcctattg gcgactcggg ttgtgaaggg
       61 tattttcccg gagatccgga atttgagatg acatggtcgg atgacagttc aaaggtttat
      121 accggggagg tggccttaga ttcctttaat tattttgcca tttgcgtcaa gttgtctgag
      181 ccgcaaagtg cctctcaacg ggagcaactc gacctgttgg aatcgtatat ggactttctt
      241 caggaagcac tggaagtgac ggggactgcg ggatatggag aggggcatac attggaatac
      301 tacccgtcgg tttccggcgt aattgattat tgggaggatg ccggaggaaa ttcctatacc
      361 gtgaaagggt ggacaaacac ccaatatctc gtagtcctgg gcatttatgg acagggcgaa
      421 tacccctatt ttaacgcagg ccaaatgtat cttgaaggta ttcgattccc ggaggaataa
      481 tcttaccttt tccgggtatt gttaagcgaa tgttacctgc ctggtgaatt ttgggtatat
      541 tagtgctatg aagcagcttt ttacactcct cctcgctgcc tgctgcactt atgctaacgg
      601 acagcttcta atcaatgaaa ttgccccgaa caactcaggg tattacgacg acgaagacaa
      661 tactttccct gactggatcg aactggtgaa tacgggtgct accactttga atgttggcgg
      721 ctacgcaatt acagacgata tggatacctg gaaccaatgg ttattaggga attacaatat
      781 tcctgccggt ggtcgcttgc gcatatttgc atctgctaaa aacagggatt gttttacatg
      841 tggcggtgtg gtttctgaaa tgcacaccaa ttttaaattg agttacggtg aaacacttgc
      901 tttgtatgac gatgccggaa atctgctcga tagtctgacc attccaaccc tgcaagctgg
      961 tcatactatg gcgcgcattc cggatggcgg cacttggtgc tattcaggaa cacctacacc
     1021 gaatgccacg aacacaggaa cttgctattc cggatatgct gcggcaccga cctttaatac
     1081 acaacccgga ttttataccg gcagtgtcga tgttactgcc agtggtgccg aagtgcgata
     1141 cacaactgat ggcgattggc ccggatttgc atcaacctta tatacaacac ctattcatgt
     1201 tactgaaaat actgtagtga aactcaccgg tattgaagca ggtaaactcc caagcagagt
     1261 tgttacagga tcattcttta tagatgagcc aaccttatta cctgttgtaa gtatcagttc
     1321 taacagctgt gatatgtttg atgaaggtgc aagttgcata gcggcgtacg acggagcaga
     1381 tggctgggaa cctgataacc cgcaaatcag cgcagcggta gaatatttta ctgccgataa
     1441 agtgcagcaa ttcgaaaaac gaatcaaatt tgaaactgca ggaaattcat cgattttcgt
     1501 ttatccgcaa cgaggtatgc aatttacatg cgatgaagat tttggtgaaa caagtgaatt
     1561 tcaattcaat ttatttgctg cttcaaaacc cgatctcgat tcgttgaacg gtttcagagt
     1621 gcgcgcaaac aatgactggg gaaactctgc agcacgcatg aaggatgtga tcaataaccg
     1681 catcgcctta cctactaata ttgtagtacc tgcttatcaa aacgtagcca catttatcaa
     1741 tggagaatat tggggacatt attctgcccg cgaagaactt gatgcatatt tcctgagaaa
     1801 taatcttggt gtaaatcccg ataaagttga tctcattcga tcaggcgccg gtgaagatgt
     1861 ttgggatatt gcggaattcg gcacgatgac cgcctacaat gaattgtcga ctttctttaa
     1921 cacgcataat atggcagatc ccgatgatta tgcattggca ttgggattga tagataaaga
     1981 aaactggatc gatcattttg cattgcagtt ttttgtgaac aatgacgaaa tggcatacaa
     2041 tttacgctgc ttcagatcgt atgatcctga catgaaatgg aaatttattt tatgggatac
     2101 aggggcagga agtgaatgtg aaacatgcaa cagcattcaa tcactcatta actttccata
     2161 tctgagtgat gagattaaca tgatgaatga cttgatggat aatgaggatt tcaaaattga
     2221 ctttatcaat cgttatgccg atctgatgaa ttattatttc acctgggatc gcatcgaatc
     2281 gctaatcgat gtaaatgccg atgaaattga agctgaaatt ccggcacaaa acgcacgctg
     2341 gggaacggga actctcgcaa cgtggaataa cggagtatca gaattgaaag catttttcga
     2401 caaccgagta tattaccagc gcaatgagat agagaattac ctcgacatga atgatcaggt
     2461 aaatgttaca ttggaagtat ctcctgccgg tgccggatat attaaaattt ctaccattat
     2521 cccacaagat ctcccttgga caggcgtgta ttttgatgga gcacctgtta ccgttaccgt
     2581 gatggcaaat ccgggttata ctttcagcaa ctggacagac aatccattta ttgccagcga
     2641 aacagatcca tctttcactg taaatatcac tgagaacact acgttcactg ccaactttac
     2701 aggtgccgcc attgcgaatc cgatttatat cagtgaaatt aattataaca gcgactcaag
     2761 cttacaatca ggtgattgga tagaaattca caatactgca ggtgtagctg ttgatgtaag
     2821 taactatact ttcagcaata attatttcta taataaattt ctaatccctt ccggcactat
     2881 cattcctgca aacggatttt tggttctggt agaagatact gcattattca cttcgatgca
     2941 tcctgatgtt tcttcgtttg ttggaccatt gcatttccca attcgcaacg aaggcgatag
     3001 tgtaaccttg cgaaacgccg ttggtgaagt attaatgtca tttgcattca atgacaagcg
     3061 cccttggccg atcaccgcag atggttatgg tagaacatta gaattcccat attattttac
     3121 cgatccgtca ttgcctgaaa actggcttgc aggatgtata ggaggctcgc ccggattgcc
     3181 atatacacct tgcattgaaa atccagtgat agaagaaatc aattacaaat ctgctattac
     3241 tgaagatgca ggcgactggt ttgagtttta caattgggca gcgtcgccgt tcgatttgag
     3301 tggttggaaa gtgatggata aaaacaaaaa cgtatttacc attccaaatg gaacgcaaat
     3361 tcccggaggt ggcggatatt atgttgttta tcaggatgaa acaaaattct ttgctcaatt
     3421 cccaactgtt acaaatgcaa cgggacctac aatttttgga tttgatggtg agggtgatgt
     3481 catttccatt tttgatcaag atggtcaatt gcgttacagc attggctacg acgatgaggc
     3541 tccttatccg ctcagtcctg atggtggcgg tactgctttg caaatgataa acactgcatt
     3601 gaatggcaat gaccctgcaa actggacaga gagttgtcct gaaggaagtc cgggtaccga
     3661 gtttgtaatg ccttgcgcca acagtatcga acaacaagaa cctgagttga gtttacaagt
     3721 atcaccaaat ccgggatcga cattatttaa tatcagcatt cccggaatga ccggtgaagc
     3781 aagtatactc attgcggatg taacaggaaa cattatttat aatggtgaat tgatacatac
     3841 caatgaattc attatcgatc tcacaaaata tcctgcaggt atatatcatg tccgtgctgt
     3901 aaatggcgat tacacctaca cagcgcaaat cgtgaagttg tagatcacct cactcccccg
     3961 cctcaccccc ggcccctctc catctcctat cgtcgataga gaggggagct gattgcgctt
     4021 cggaattaaa atattgcaaa gcaatataat tgcattcc
//
LOCUS       DAOQOU010000232         4015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_14916_length_4015_cov_4.587121, whole genome shotgun sequence.
ACCESSION   DAOQOU010000232 DAOQOU010000000
VERSION     DAOQOU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14916_length_4015_cov_4.587121"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLM90_13680"
     CDS             complement(<1..288)
                     /locus_tag="PLM90_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007051681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HMZ90482.1"
                     /translation="MILTTTNNIEGKQITDYIGIVTGETIIGANLFRDIFASVRDVVG
                     GRSGAYEEVLREAKDIALREMSEKASSMGAHAIIGIDLDYETIRNGMLMVSA"
     gene            418..639
                     /locus_tag="PLM90_13685"
     CDS             418..639
                     /locus_tag="PLM90_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90483.1"
                     /translation="MKKLALLTIATFALGALVSSCEKCTVCTAQSIDGEHVSEYCGNE
                     LDVRDFEDFFIDSLAGITETGYCERGPNN"
     gene            complement(648..1433)
                     /locus_tag="PLM90_13690"
     CDS             complement(648..1433)
                     /locus_tag="PLM90_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90484.1"
                     /translation="MAAATISSPLNDMFSADLRHAESIRKKIVLNYSIIGSVLFIGLI
                     IEAVTWMSIAILIIALFVLVYTRWYGIPVPVFEQAYVDAVTRHIISPWHGLQIDYQSH
                     LKVSELIAGGFIKEAPDYFSGRNLIHGQAGDTYLRISEVYMRSKDKSIFTQTSGEGQL
                     KALVMVADLSSDEEKQENPRTYVINNDALNTLSNQISADLHSSVFAEVRNGKLYAAII
                     HHAGFAYLNPGITKTVYHMAPIERYEKDVNALISLAINAANVQ"
     gene            complement(1445..1873)
                     /locus_tag="PLM90_13695"
     CDS             complement(1445..1873)
                     /locus_tag="PLM90_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HMZ90485.1"
                     /translation="MSNIWFDPNTRLEDFAHWGKNTLGDHLGMQFTNIGDDFLEATMP
                     VDHRTHQPYGLLHGGASVALAETIGSVASALVINHATHMSLGVEINANHLRSVRSGIV
                     TAKCTPIHIGSTIHVWNILISDAQQRPVCISRLTVMIRKR"
     gene            complement(1870..>4015)
                     /locus_tag="PLM90_13700"
     CDS             complement(1870..>4015)
                     /locus_tag="PLM90_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ90486.1"
                     /translation="NYAASEVYSQDSILLGKFFLENRTNAEFEDLPEDLIHALVSTED
                     ARFYSHDGVDFISFIRVLIMSIILNDDAGGGSTITQQLAKNLFGRKDYGPLTMPVNKF
                     REMIIAGRLEDVYSKDEILTLYFNTVPFGEDCYGIESSALRFFNKAPKDLKTEESAVL
                     VGLLKANTTYNPRLNPDKSLTRRNTVLGLMVRHENHYLTRAQADSLQALPLTINYRRE
                     STAEGLAPYFREYLKSQLEDWAKKNPKEDGSTWNIYTDGLKIYTTINGELQAYAEDAM
                     REHMASLQKQFDKSWGKTEPWKNAKTDVVNAARKRSERYQTLKDNGYSDKGIQKRFDD
                     SVKTSVFSWNGPIDTVMTPNDSLKHQLRFMQTGFLAMDPRTGYILAWVGGIDYRFFQY
                     DHVRAKRQVGSTFKPIVYATAIEQGMDPCAYIPNKKVTYESYDNWTPGNSDGEYGGYY
                     TMKGGLTHSVNTVAANLIMQTGVQPVIDEARKMGVTSKLPAVPSIALGTASISLLEMV
                     TAYSCFANRGLRADPKYIVRIESQKGDTLYEDKAAKKTRALSQETADIMIQIMRNVVN
                     AGTASRLRGTYGLGMDLAGKTGTTQDNTDGWFIGYNSRIVAGAWVGAEDPAIKWKTTA
                     LGQGAATALPIFGKFMSKSAKDADTRKYVTGGFTAPADSTLALLDCPMWIPDSLNTDS
                     LGFFQRVIGNIKDYLEKKGEDSIITKYPGAEK"
BASE COUNT         1065 a          884 c          793 g         1273 t
ORIGIN      
        1 ggctgatacc atcaacattc cattgcgaat ggtttcataa tccagatcta taccgatgat
       61 ggcatgagca cccattgacg aagctttttc cgacatttcc ctgagcgcaa tatcttttgc
      121 ttcgcgcagt acttcttcat aagcgcctga gcggccacca actacgtcgc gcacactggc
      181 aaagatgtct ctgaagagat ttgcgccaat aatggtttca ccggttacta taccgatgta
      241 atctgtaatt tgtttgcctt caatgttgtt tgtggtcgtt aaaatcatgt ccgggttgtt
      301 tacttgatac atatgcctgt tttacaaata tacatgttga tatctgcaat gacgtaataa
      361 ggcctccgat attgtcgttt gtcttgtatt cgtatatttg tatccaaact gctgaaaatg
      421 aaaaagctcg ctctcctcac aatcgcaact ttcgcattgg gcgctttagt gtcatcttgc
      481 gaaaaatgta cggtttgtac cgctcaaagt attgacggtg agcatgttag tgaatattgc
      541 ggaaacgaac tcgacgtgcg cgattttgaa gatttcttca ttgatagcct cgcaggcatc
      601 acggaaacag gttattgcga gcgcggacct aataattaag catttgctta ttgcacattt
      661 gctgcgttga tcgccagtga gatcaatgca tttacatctt tctcataacg ctctattgga
      721 gccatgtggt aaaccgtttt tgtaatgccg ggatttaaat aggcaaaccc tgcatgatgt
      781 atgatggcag catatagttt cccatttcgc acttcagcaa atacgctgct atgcaaatcg
      841 gcacttattt ggtttgataa tgtattcagt gcatcattat tgatcacata ggttctcggg
      901 ttttcctgct tttcttcatc tgatgacaaa tcggcaacca taaccaatgc ctttaattgc
      961 ccttcgccgg atgtttgtgt gaatatcgat ttatcttttg accgcatata cacttccgaa
     1021 atgcgtaaat aggtatcacc ggcttgtcca tgaatgagat ttcttcctga aaaataatcc
     1081 ggcgcttctt tgataaatcc accggctata agctcactca cttttaaatg actttgataa
     1141 tcaatttgca atccatgcca gggagaaata atatgtcgcg ttacggcatc cacatacgct
     1201 tgctcaaaca cgggtacagg aatgccatac catcttgtat ataccaacac aaataatgca
     1261 ataattaata tggcaatcga catccatgtc accgcttcta tgatcaatcc gataaataat
     1321 acactgccga ttatgctgta attgagcaca atctttttcc tgatgctttc tgcatgtcga
     1381 aggtcggcag aaaacatgtc gtttagcgga gatgaaattg ttgctgctgc cataatatga
     1441 tgatttagcg ttttcgaatc attaccgtaa gtcgactaat gcataccggt ctttgttgtg
     1501 catcgcttat aagaatattc cagacatgaa tggtgctgcc gatatgaatt ggtgtgcatt
     1561 ttgcggtaac tattcctgat ctgacactgc gcaaatgatt ggcatttatt tctacaccaa
     1621 gcgacatatg tgtggcatga ttaattacca atgcagatgc cacgctgcca atggtttcgg
     1681 ccaatgctac agatgctccg ccgtgcaata acccgtatgg ttgatgtgtg cgatgatcta
     1741 caggcatggt agcctccaga aaatcatcgc caatattagt gaactgcata cccaaatgat
     1801 cgccgagtgt atttttcccc cagtgggcga aatcttcaag acgagtattc ggatcaaacc
     1861 agatgttact catttttctg cgccggggta ttttgtgatg atggaatctt cacctttttt
     1921 ctcaagataa tctttaatat ttccaatcac gcgctgaaag aatccaagtg aatcggtatt
     1981 caaagaatcc ggaatccaca tcggacaatc taacaatgcc agtgttgaat ctgcaggtgc
     2041 cgtaaatcca cctgtaacat attttctggt atcggcatcc ttcgcgcttt tcgacataaa
     2101 ttttccaaag atgggtaggg cagtggcagc accttgtccg agtgcggtag ttttccattt
     2161 aattgcaggg tcttcagcgc ctacccatgc tccggcaacg atgcgcgagt tatatccgat
     2221 aaaccagccg tcggtattgt cttgagtcgt gcctgttttt cctgcgaggt ccattcccaa
     2281 tccataagta ccacgcaatc gcgaagcagt gcctgcattt accacattgc gcatgatctg
     2341 tatcatgata tcggcagttt cctgacttag tgcgcgtgtt tttttcgctg ctttatcttc
     2401 atataaggta tcgccttttt gtgattcaat gcgcacgata tatttcggat cggcacgcaa
     2461 gccccgattt gcaaaacaag aataggcagt aaccatttcc agtaatgaaa tactggcagt
     2521 tcctaatgca atactaggta ctgcaggtaa tttactggta acacccattt ttcttgcttc
     2581 atcaattacc ggttgcacac ctgtttgcat aatcaaattt gcagcaacgg tatttactga
     2641 atgcgtgagt ccgcctttca tggtataata gccgccgtat tctccgtcag aatttcccgg
     2701 tgtccaatta tcgtatgatt cgtaagtgac ttttttattt ggaatatacg cacaaggatc
     2761 catgccttgt tcaattgccg ttgcataaac aataggttta aatgtagaac caacctgacg
     2821 ttttgcccgc acgtgatcat attgaaaaaa gcgataatct attccgccta cccatgccaa
     2881 aatatatccg gttctgggat ccatcgctaa gaatccggtt tgcataaatc gcaactgatg
     2941 cttcaacgaa tcatttggtg tcatgactgt atctatgggt ccattccatg aaaatacact
     3001 cgtttttacg gagtcgtcaa atcgcttttg aattcccttg tcactgtaac cgttatcttt
     3061 taatgtctga tagcgttcac tgcgtttgcg tgctgcattt actacgtcag tttttgcatt
     3121 tttccagggt tctgtttttc cccagctctt atcaaattgt ttttgaagtg atgccatatg
     3181 ctctcgcata gcatcttcag cataggcctg caattcaccg ttgatggtag tatatatttt
     3241 caatccatca gtataaatat tccatgtact gccatcttcc ttcggatttt ttttcgccca
     3301 atcttctaat tgtgatttaa gatattctct gaaataaggt gcaagacctt ctgccgtgct
     3361 ctcgcgacga taattaatgg tgagtggtaa tgcctgtaaa ctatcagcct gcgctcttgt
     3421 caaataatga ttttcatggc gcaccattaa tccaagtact gtatttcttc tggtgaggga
     3481 tttatccgga tttaatcgcg ggttatatgt agtgttggct ttcagtaaac caacgagtac
     3541 tgcactttct tcagttttga gatctttcgg agctttatta aaaaatcgta aagcggaaga
     3601 ctctatacca tagcaatctt caccaaatgg aaccgtatta aaatagagtg taagaatttc
     3661 atcctttgaa taaacatctt ccaggcgccc tgcaataatc atttctctga atttattcac
     3721 gggcatggtt aagggaccgt agtcttttct accaaagaga tttttagcaa gttgttgtgt
     3781 gatagtacta ccgccacccg catcatcatt taaaatgatg gacatgatga gcacgcgaat
     3841 aaagcttata aaatctacac catcatgcga ataaaatcgg gcatcttcag tagaaaccaa
     3901 agcatgtatc agatcctcgg gaaggtcttc gaattctgcg ttggttcgat tttctagaaa
     3961 gaatttaccc agcaatatgc tatcctgtga atacacttca gaagcagcgt aattc
//
LOCUS       DAOQOU010000233         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_15303_length_3958_cov_7.290290, whole genome shotgun sequence.
ACCESSION   DAOQOU010000233 DAOQOU010000000
VERSION     DAOQOU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15303_length_3958_cov_7.290290"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLM90_13705"
     CDS             complement(<1..318)
                     /locus_tag="PLM90_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90487.1"
                     /translation="MKSLSYLSFICIMLLSACSVKPHPINEGEDACAYCKMTVAEKKY
                     ATEIVSNTGKIYVFDDMHCMKSFIKENIVPSGDIKLMMVSDFYNPENLIPADNALLFT
                     SESL"
     gene            complement(320..916)
                     /locus_tag="PLM90_13710"
     CDS             complement(320..916)
                     /locus_tag="PLM90_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90488.1"
                     /translation="MKKIHNYSAIAVAIAALCFLATFKFPFWVIYLTAPQYPEGLTLY
                     IWLHKVSGDVEIINGLNHYIGMAIIDETQFPEFDILPYAIGFMVGAGLLIAFLRKKKL
                     LFFYTIFLVLFSILAIYDFWHWEYQYGHNLDPDAAIQVPGMSYQPPLIGFKQLLNFGA
                     YSFPHGGGFFYISGVVICCIASILEWRRGKKQLTPKIR"
     gene            complement(1010..1570)
                     /locus_tag="PLM90_13715"
     CDS             complement(1010..1570)
                     /locus_tag="PLM90_13715"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HMZ90489.1"
                     /translation="MKKLLYILPLCLALQFGCNNAESTEASGNNDTMNAQAGGQEAVQ
                     DDVSQKDVVKIAVGSPDHTTLVAALKQTEYVNDLSNAGPFTVFAPTNAAFDKLPAGTV
                     DDLMKDENQAALRNILEYHVALGTYRKEYLNNGQTIGMANGDNVTIGINGDDVTVNGN
                     KVLAIVPASNGIIYIVDGVLLPPAKK"
     gene            complement(1608..3584)
                     /gene="nosZ"
                     /locus_tag="PLM90_13720"
     CDS             complement(1608..3584)
                     /gene="nosZ"
                     /locus_tag="PLM90_13720"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HMZ90490.1"
                     /translation="MKNLTLMLLVTMLTIAGCKPKTGTSVASGDAASKVYVAPGTYDE
                     FYNFVSGGFSGQLAVYGIPSGRLFREIPVFSEDPETGWGYTEETKPMLMTSHGYVPWD
                     DFHHPALSTTNGVHDGRWAFGNANNTPRIARIDLKTFRTAEIIEIPNSSGNHSSPFIT
                     ENTEYTIAGTRFSLPPDYEGANTDVSISSYKDNFKGYLSFISVGKETGNMDVAFQIQI
                     PPLNTDLARGGKGPSHDWFFFSCYNSEKAFTLLEVNASQKDKDFILAVNWKKAEEYAK
                     AGKGQHQKVKYTHNTYDEKTHSATSEIKTDVLVLDPKELKDVLYLIPCPKSPHGCDVD
                     PTGQYIVGSGKLAALIPVFSFEKIQKAIANNDFEAEYDGIPVLKYESCLHGEVQKPGL
                     GPLHTEFDDQGNAYTSFFVSSEIVKWNVKSLQVLDRVPTYYSIGHLCVPGGPTAKPWG
                     KYVIAYNKITKDRYLPTGPELTQSAQLYSIEGDKMKLLLDFPTTGEPHYAEAIPASLV
                     QPNSTKIFKIEENNHPYAAKGEKEAKVFREGNIVHVVMTSIRSHFVPDNIEGVKMGDV
                     VYFHVTNLEQDWDVPHGFVVKGANNAEMLIMPGETQTLKWEPDRVGVFPFYCTDFCSA
                     LHQEMQGYIRVSPAGSSVPLTFSTGKTAAEPPAK"
     gene            complement(3623..>3958)
                     /locus_tag="PLM90_13725"
     CDS             complement(3623..>3958)
                     /locus_tag="PLM90_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ90491.1"
                     /translation="RGIGEVKSVTLNTPLEQERVKRGQDIYDMKCSACHKLTDERFVG
                     PGWKGVTSRRKPEWIMNMVTNVDVMLERDAEAQKLLELCLTRMPNQNVSIGDARDILE
                     FMRSNDGEK"
BASE COUNT         1097 a          800 c          820 g         1241 t
ORIGIN      
        1 caatgattct gatgtaaata gcagtgcatt atcagcaggg ataagatttt ctggattata
       61 gaaatcactt accatcatga gtttaatatc gcctgaaggt actatgtttt ctttaatgaa
      121 tgatttcatg caatgcatat cgtcaaaaac atatatttta ccggtattgc ttactatttc
      181 tgtagcgtat tttttttcgg ctactgtcat tttgcaatat gcgcaggcat cctccccttc
      241 attgattggg tgaggtttga cagagcaagc actcaatagc attatgcaga taaagcttaa
      301 atatgataat gatttcatgt tatcttattt taggagtcaa ctgttttttt ccacgcctcc
      361 actcaagaat ggatgcgatg caacaaatca ctacaccgga gatataaaag aatccacccc
      421 cgtgagggaa tgagtatgca ccaaagttta ataattgttt gaagccgata agtggtggct
      481 gatagctcat gcccggtacc tgaattgcag cgtccggatc aaggttgtga ccatattgat
      541 attcccagtg ccagaaatcg taaatagcaa ggatagaaaa taacaccagg aaaatggtat
      601 agaagaacag caatttcttt ttccttaaaa aggctattag caatcccgct ccaaccataa
      661 atcctattgc gtagggcaga atatcgaatt cagggaattg agtttcatca ataattgcca
      721 tcccaatata gtgattcaat ccatttatga tttctacatc accacttacc ttgtgtaacc
      781 aaatatagag tgtaagcccc tccggatatt gtggtgcggt gagataaatt acccaaaaag
      841 gaaatttaaa ggttgccaga aaacaaagtg cggctattgc tacagctatt gcggaataat
      901 tgtgaatctt tttcatatgc cgtggtttaa caaccgggat acggtattgc atcccggttg
      961 tgattttcca aacactcaac cattaagacc ggttgcacat tgcaacctat tattttttag
     1021 caggaggcag taatacgcca tccacaatat aaataattcc atttgaggcg ggaacaatag
     1081 cgagcacctt gttgccgtta acggtaacat catcgccatt aataccaatg gtaacattgt
     1141 caccatttgc catgccaatt gtttgcccat tgttcaggta ttctttgcga taggtaccaa
     1201 gagcaacatg gtattccaga atattgcgca gggcagcctg gttttcatct ttcatcaggt
     1261 catcaacagt tcccgcaggt aatttatcaa atgcggcatt tgttggtgcg aatacagtaa
     1321 atggaccggc attgctcaaa tcgttgacat attctgtttg tttaagggct gctaccaaag
     1381 tggtatgatc cggtgagcca actgctattt ttacaacatc cttttgtgaa acgtcgtctt
     1441 gaacagcttc ctgtccgccg gcttgtgcat tcattgtatc gttgttacca cttgcttctg
     1501 tgctctctgc attattgcaa ccgaattgca atgccaggca taatggtaaa atatataata
     1561 gctttttcat aatatgtgtt taatcggtat ggtaattctt gctcaattta ttttgccggt
     1621 ggttctgctg ctgttttgcc tgtactgaaa gtaagtggta cagatgaacc ggcaggcgat
     1681 acgcgaatat atccttgcat ttcctgatgc agtgctgagc agaaatcggt gcaatagaat
     1741 gggaatacgc caacgcgatc aggttcccac ttcaatgttt gggtttcgcc gggcatgatc
     1801 agcatttcgg cgttgttagc tcctttcact acaaatccat gtggtacatc ccagtcttgc
     1861 tcaaggttgg tgacatggaa gtatactaca tcacccattt taacaccttc aatattatca
     1921 ggcacaaagt gagagcgtat tgatgtcatt accacgtgaa caatatttcc ctcacggaaa
     1981 acttttgctt ctttttcgcc ttttgctgca tatgggtgat tgttttcctc aatcttgaaa
     2041 attttggttg aattaggttg taccaatgat gcaggaattg cttcggcata gtgtggctca
     2101 cctgttgtcg ggaagtcgag caataatttc atcttatcac cctcaatgct atataactga
     2161 gctgattgag ttaattcagg tccagtaggc agataacgat cttttgtgat tttgttatac
     2221 gcgatcacat atttacccca aggtttggct gttggtccgc cgggaacaca aaggtgacca
     2281 attgaataat aggtaggaac gcgatcaagt acttgaagtg attttacatt ccatttaacg
     2341 atctcactcg aaacaaagaa tgatgtatag gcatttcctt gatcgtcaaa ttctgtatgc
     2401 aatggaccta aacccggttt ttgtacttca ccatgtaaac aactttcata ttttaatacc
     2461 ggaataccat catattctgc ttcaaaatca ttattagcaa tagccttttg aattttttcg
     2521 aatgaaaata caggaatcaa agcagcaagt ttacctgatc caactatgta ttgacctgta
     2581 ggatcaacgt cacaaccatg tggtgatttt ggacaaggaa taagatataa aacgtctttt
     2641 aattcttttg gatctaaaac cagcacatct gttttaattt ctgatgtggc agaatgcgtt
     2701 ttttcatcat atgtgttgtg ggtatatttt actttttgat gctggccttt tcctgcttta
     2761 gcatattctt cagctttttt ccagttaacg gcaagaataa aatctttatc tttctgacta
     2821 gcatttactt caagtaacgt gaaggctttt tcagaattgt agcagctgaa gaagaaccaa
     2881 tcgtgtgatg ggccttttcc tcctcttgca aggtcggtat ttaatggtgg tatctgaatc
     2941 tggaaggcta catccatatt tcctgtttct ttaccaacgc tgataaaact cagataacct
     3001 ttgaaattat ccttataaga agaaatggat acatcggtgt ttgcaccttc ataatcagga
     3061 ggcaagctaa agcgagtacc tgcgatagtg tattctgtat tttcagtaat aaagggagag
     3121 ctgtgattac cggaactatt tggaatttcg ataatctcag cagtgcggaa tgttttcaaa
     3181 tctatacgag caatacgcgg tgtattattg gcatttccaa atgcccatct gccgtcgtgc
     3241 acgccattgg tagtagagag cgcagggtga tggaagtcat cccaaggtac ataaccatgt
     3301 gaagtcatga gcatgggttt tgtttcttcg gtgtatcccc atccagtttc aggatcttca
     3361 gagaacacgg gaatttcgcg gaaaagtcgt cctgacggaa tgccataaac ggcaagctgg
     3421 ccgctaaaac cgccggatac gaaattgtaa aattcgtcat atgtaccggg tgctacatat
     3481 acttttgaag cagcatcgcc tgatgcaaca ctggtacccg ttttgggttt acatcccgct
     3541 atggtaagca tggttaccaa tagcataagg gttaggtttt tcatagtaag ttatggttta
     3601 atgtagaatg atcaaataca atttattttt ctccatcgtt gctgcgcata aactcaagaa
     3661 tatcgcgggc gtcgccgatc gaaacatttt gatttggcat gcgggtgaga catagctcga
     3721 gcagtttttg ggcttcggca tcgcgttcaa gcatgacgtc gacattagtg accatattca
     3781 tgatccactc cggtttgcga cgtgaagtaa ctcctttcca acccgggcct acaaagcgct
     3841 catcggtgag tttgtggcag gcagagcatt tcatgtcata tatatcctga ccacgtttta
     3901 ccctttcttg ctcaaggggg gtattcaggg taacagattt cacttcgccg attccgcg
//
LOCUS       DAOQOU010000234         3896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_15756_length_3896_cov_5.718563, whole genome shotgun sequence.
ACCESSION   DAOQOU010000234 DAOQOU010000000
VERSION     DAOQOU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15756_length_3896_cov_5.718563"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /gene="rfbC"
                     /locus_tag="PLM90_13730"
     CDS             complement(<1..335)
                     /gene="rfbC"
                     /locus_tag="PLM90_13730"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HMZ90492.1"
                     /translation="MPFIETPISGLYVYEPKVFEDARGYFYESFNAQVFGEKGINTNF
                     VQDNQSRSSYGVLRGLHYQLAPHAQAKLVRVISGEVYDVAVDIRKGSPTFGQHFGVIL
                     STENKKQLY"
     gene            complement(410..1162)
                     /locus_tag="PLM90_13735"
     CDS             complement(410..1162)
                     /locus_tag="PLM90_13735"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HMZ90493.1"
                     /translation="MSSVFGRFFSGNKTEHTDFSALGADMHAHIIPGIDDGSQDVATS
                     IAMIKGLQELGFSKFISTPHIMPGVFNNDEESIRAATELLNQSAPKETGTTVQYAAEY
                     YIDDDFPQMVNSGKPLLTFGDQYVLVEANMMSRTRNMEEALFELRLRGLRPILAHVER
                     YPYMYEHHRLDQYEKLRDADVLLQVNLRSFTGNYGEIQKRIARHLADAGMISFLGTDL
                     HNPAQLAIIRESMLDQHVQNLLKSGDLLNNTL"
     gene            complement(1164..2225)
                     /gene="rfbB"
                     /locus_tag="PLM90_13740"
     CDS             complement(1164..2225)
                     /gene="rfbB"
                     /locus_tag="PLM90_13740"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HMZ90494.1"
                     /translation="MNYQKTILVTGGAGFIGSHVVRLLVNKYPAYRIINLDKLTYAGN
                     LNNLSDVEHADNYAFVKGDITDAGFITSLFEEHRFDMVVHLAAESHVDRSIANPIEFV
                     ITNVVGTCVLLNAARNIWKDDYSGKRFYHISTDEVYGTLGESGLFTETTPYDPHSPYS
                     ASKASSDHFVRAYHDTYGMPVVISNCSNNYGSFQFPEKLIPLAINNIKNMKPIPVYGK
                     GENVRDWLWVEDHARAIDVILHNGKNGDTYNIGGFNEWKNIDLIHLLCRIMDNKLQRE
                     PGTSAKLITYVTDRPGHDMRYAIDAQKLNRELGWKPSVDFEQGLEKTVEWYLANQHWL
                     EQVTSGAYKQYYEQQYATN"
     gene            complement(2222..3511)
                     /locus_tag="PLM90_13745"
     CDS             complement(2222..3511)
                     /locus_tag="PLM90_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMZ90495.1"
                     /translation="MIEQLRNKHARISVVGLGYVGLPIALEFGRHFHVTGFDIDPTRI
                     EALKNNVDPGGELPASYFENSTVDFTTNIDALKHCNFHIIAVPTPVDEHKVPDLSSLQ
                     SATQTIASILKKGDYVVFESTVYPGCTEDICLPILESVSGLKVNTDFKLGYSPERINP
                     GDDVHTLRNVMKIVSGSDKEALETISDVYVSIVQPGVYQASSIKVAEAAKILENTQRD
                     LNIALMNELSIILDKMGVNTYEVIEAAGTKWNFHKYKPGLVGGHCIGVDPYYLTYKAM
                     ELDYEPYVILSGRKVNDNMAYHVAKRCVQKLIEAGKNLATSRILVMGITFKENVNDIR
                     NSKVADMIKELRSFGLQVDVVDPKADSAEVEKHYGFELQKVNGHQYDAIILAVPHKEY
                     VQLTPEYFASIATTDGIFIDIKGIFRDKINTLQYWSL"
     gene            complement(3521..>3896)
                     /locus_tag="PLM90_13750"
     CDS             complement(3521..>3896)
                     /locus_tag="PLM90_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ90496.1"
                     /translation="NYNIRRIASADQYDALLNVIPGITIPTRVQNATHVFHQYTIRVK
                     KHRDALKDHLQSKGIPSMIYYPVPLHVSDAYRIYGYQPGDFPVSEMVATEVLSLPMHS
                     ELDAEQINFICGEIAAFMQANA"
BASE COUNT         1089 a          848 c          776 g         1183 t
ORIGIN      
        1 atatataatt gctttttatt ttccgtagat aatatcactc caaaatgctg accgaatgtt
       61 ggtgagcctt tgcggatatc aacagccaca tcataaacct caccgctgat tacgcgcacc
      121 agttttgcct gtgcatgcgg tgccagctga tagtgcaatc cgcgcagcac gccatacgac
      181 gagcgactct gattgtcttg cacaaaattg gtattgatgc ccttttcacc aaatacttgc
      241 gcattaaatg attcataaaa gtaaccgcgg gcatcttcaa atacctttgg ttcatataca
      301 tacagtccgc ttataggtgt ttcaataaaa ggcatcaatt ttcaatcagg ctgcaaaaat
      361 accccgttct gcccgaaaac gggtagtatg tggaatggtt gtagacaatt tagagggtat
      421 tattgagcaa atcgccgctt ttcagcaggt tttgcacgtg ctgatccaac atggattccc
      481 ggataatggc caattgagcc ggattatgca gatcggttcc cagaaagctg atcattcctg
      541 catcggccag atggcgggct atccgttttt gtatctcgcc ataattaccg gtaaatgaac
      601 ggaggttcac ttgcagcaac acatcagcat cacgcaattt ttcatattga tctaatcgat
      661 gatgctcata catgtatgga tatcgctcga catgcgccag tatcggacgc aatccgcgca
      721 agcgcaattc aaacagtgcc tcttccatgt tgcgtgtgcg cgacatcata ttagcttcca
      781 ccagcacata ttgatcgccg aaagtcagca gtggtttacc actgtttacc atttgcggaa
      841 aatcatcatc gatgtaatat tctgccgcat attgaactgt tgtacccgtt tccttaggtg
      901 cagattgatt gagtaattca gttgccgcac gaatactttc ttcatcatta ttaaacacac
      961 cgggcataat atgaggtgtg ctaatgaatt tagagaatcc caattcctgt aaacctttta
     1021 tcatggcaat tgaagttgcc acgtcttgtg atccatcgtc gattccggga atgatatgtg
     1081 catgcatatc tgctcccagc gcactgaaat ccgtgtgttc ggttttattc cctgagaaga
     1141 atctgccaaa cactgagctc atattaattt gttgcgtact gttgctcgta atattgttta
     1201 taggcaccgc ttgtaacctg ctctaaccaa tgctgattcg caagatacca ctctaccgtt
     1261 ttttccaaac cttgttcgaa atcaacagaa ggtttccatc ccaactcgcg atttaatttt
     1321 tgcgcatcga tggcatagcg catatcatga ccgggacgat cagttacata agtgatgagc
     1381 tttgcagatg tccccggttc gcgttgcagt ttattatcca taatgcgaca gagtaaatga
     1441 atgaggtcga tatttttcca ttcattaaat ccgccgatat tgtaggtatc tccatttttt
     1501 ccgttgtgca gaatcacatc aatggcgcga gcatgatctt ccacccacag ccaatcgcgc
     1561 acattttcgc ctttgccata aacaggaata ggcttcatgt ttttaatgtt atttatggct
     1621 agcggaatta atttttcagg aaactgaaat gatccgtaat tattgctgca atttgagatt
     1681 accaccggca tgccataagt atcgtggtaa gcgcgaacaa aatggtcgct ggatgctttg
     1741 ctggcagaat acggactatg cggatcgtaa ggtgttgttt ccgtaaataa gccggactct
     1801 cctaatgttc catatacttc atcggtagag atatgataga atcgttttcc ggaataatca
     1861 tccttccaaa tattcctcgc agcatttaat aatacacatg taccaaccac attggtaatt
     1921 acaaattcaa tagggtttgc aatactgcga tctacatggc tttcagcggc aagatgcaca
     1981 accatatcaa agcgatgttc ttcaaaaagc gaagtgataa atccagcatc tgtgatatca
     2041 ccttttacaa atgcataatt atcggcatgt tcaacatcag agagattatt caaattgccg
     2101 gcataggtca atttatcgag gttgatgata cgatatgccg gatatttatt taccagcagt
     2161 cgcaccacat gtgaaccaat aaatccggcg ccgccggtaa ctagaatagt tttttgataa
     2221 ttcataagct ccagtattga agggtattaa ttttatctct gaatatacct ttgatatcaa
     2281 taaaaatacc gtcggttgtt gcaatagatg caaaatattc aggtgtgagt tgcacatatt
     2341 ctttatgagg cacagcaagt ataatggcgt catattgatg accatttact ttttgcagtt
     2401 caaatccata atgcttttcc acttccgcac tatcggcttt tggatctacc acgtctactt
     2461 gtaaaccgaa tgaacgcaat tccttgatca tatcggccac ttttgaattg cgtatatcat
     2521 tgacattctc tttgaacgtg atacccatga ctaatattct ggaggtagcc aaattttttc
     2581 ctgcttctat gagtttttgc acgcagcgct tagccacatg ataggccata ttgtcattta
     2641 cctttctgcc gctcaaaatc acataaggtt cataatcaag ctccatggcc ttataggtga
     2701 ggtaatacgg atcaacgcca atgcaatgcc cgccaacaag tcccggttta tatttatgaa
     2761 aattccattt cgtgcctgca gcttctatca cttcataagt attcacgccc attttgtcga
     2821 gaatgatact gagttcattc atcagcgcaa tattgagatc gcgctgcgta ttttcaagaa
     2881 ttttagctgc ttctgcaacc ttaatagatg atgcctgata aacaccgggt tgcacgatgg
     2941 aaacatacac atcactaatt gtttcgaggg cttctttatc cgagcccgat acaatcttca
     3001 tcacattgcg caatgtgtgc acgtcgtcgc ccggattgat gcgttcagga gaataaccga
     3061 gtttgaaatc tgtatttact tttaatccgg atacagattc aagaatgggc aaacaaatat
     3121 cttctgtaca tccggggtaa acagtagatt cgaatactac ataatcgccc ttttttaata
     3181 ttgaagcaat tgtttgtgtt gccgactgaa gactggaaag atcaggcacc ttatgttcat
     3241 caacaggtgt tggcactgcg atgatatgaa aattgcaatg ttttaatgca tcaatgtttg
     3301 ttgtaaaatc aactgtggaa ttttcaaaat aggaggccgg tagttcgcca cccggatcta
     3361 cattattttt taatgcttca atgcgcgttg ggtcgatgtc gaaaccggtg acatgaaaat
     3421 gtctgccaaa ctctaaggct attggcaaac ccacatagcc aagacctact acagagatgc
     3481 gtgcgtgttt attcctgagt tgttcgatca tggcgatggt ttaagcgttt gcctgcatga
     3541 aagcggcaat ttcgccacat ataaaattta tttgctcggc atctaattcc gaatgcatgg
     3601 gcagcgataa tacttccgtt gccaccattt cgctcacagg aaaatcgccc ggttgatatc
     3661 cgtaaatacg ataagcatca ctcacatgca acggcaccgg ataatatatc attgatggta
     3721 tacctttcga ttgcagatga tctttcaagg catcgcgatg ttttttaacg cgaatggtat
     3781 attgatgaaa tacatgcgta gcattttgta cgcgagttgg tatagtaata cccggaataa
     3841 cattcaataa tgcatcatat tgatcggcag atgcaatacg gcgtatattg taatta
//
LOCUS       DAOQOU010000235         3896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_15758_length_3896_cov_4.400677, whole genome shotgun sequence.
ACCESSION   DAOQOU010000235 DAOQOU010000000
VERSION     DAOQOU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15758_length_3896_cov_4.400677"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1063
                     /locus_tag="PLM90_13755"
     CDS             176..1063
                     /locus_tag="PLM90_13755"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90497.1"
                     /translation="MQKELVKRLTDYAAFAAAVLVLINRADAEVVYTDIDPDVSLDTD
                     GNYYAIDIDGNGTADFQFLKATFSINPSFTYYYFFQELVAGPFSQNALAGSTGISFYA
                     GSILYFPFALQMDSIINEKLTWQSATFQFLAWKTWTDDYDNHCNNCFWNTKDLYEVLD
                     HYLAIRFVDTENNNHYGWIRCDVKEDGSILILKDYAYETEPDYPILTGAKTTYQEITP
                     GNIEGEIYASGNTVHISVPRRPTSEYTVQIYNMSGQLVVASQFDSPFVEIQIDVPRGI
                     YIVRVTSANEQLSKEVLIE"
     gene            1374..>3896
                     /locus_tag="PLM90_13760"
     CDS             1374..>3896
                     /locus_tag="PLM90_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90498.1"
                     /translation="MKKSIIHKIRFLNVTLLFIILITGITLAARFAGDNIRYNTSESS
                     TTTFIPPLPDSTYIDSTDFSGIYDTAYTYSDGGDSVYVVADSVFTDSIFSDTSLTEGR
                     FGSSGNFIRNAINYSSAKVGSGTGPIVCEPLFDGSSPELINKGLYGINVSGMFDNATC
                     PNDGSATDQWDWLSDLRPEVLRFPHGSNGKFMHLLHDVSTGDTAAGYGYDIAELIRYF
                     DASNNDNTDLVPGIYPDMATALATISTQSEANLAEWNTWMDNTVSGQFGKFLAKWQEQ
                     QQLVTTATDHRNYLTDFIKLIRQIETDNPGHTVKIIVCLNILSETSAKCSAIVQFLRS
                     NPIHNCTVVGVELGNECPSDFHWKVMGFAHFSEHDTPDGCDEAFNGSYWSFINGEAYE
                     TGDAQDRLDLFLNPAMQLQDAAGHYYNRDFIKAFKYNFLTSCKIGIPGEGTPNDELLP
                     PPFFLLTGCPRGATEWNTDLTSQYGEEIASMPPGLAKKKFDAVILHIYLDDKQNWGPP
                     IADLLLPYPYSERWDYASFDTRLQAAFNGILNFDPADPGSPASDSFMGFIKYGHKTAW
                     DDYKTVFNFTPTAPTDKLRKELWMTEYNIKTSVDEDNLQRGVFTNGLTHGFVLQEWFL
                     KNLKVNFASGYKKNFFTYATYQNYAGVGEDLITPADRPRELYNYLCKDYSPYNLGGGN
                     PNQRDYYMKRTSYFIMSLLKEISAQDLKYVRTRYATILSNMNTMPTMFIDPGNDFLYM
                     FYTNMKSTEQDYIINGENLAALFTPTPIISFGTATIKNIHALQLYSGSGNSTLFNPLF
                     NTGYTNPTPADRTCYEGELLPPAYLYPIEIQTTSFSVNAPDCA"
BASE COUNT         1175 a          784 c          770 g         1167 t
ORIGIN      
        1 caagtgaata ttttacttta caaatattca acctgcaggg agatttgatg atggcgcaaa
       61 gtgttacctc agctaaaagc atttcaatta aaacactgcc ggcaggtttt tatttgcttt
      121 catttaccga ccctcaggga attattatca ataagcaatt tgtaaaaatc gaataatgca
      181 aaaggaactt gttaaacgat tgacagacta tgcggcattt gcggcagcgg ttcttgtttt
      241 aattaacagg gctgatgcag aagtggtgta taccgacatt gatcccgatg tttcgcttga
      301 cactgacggt aattactatg caatagatat tgatggaaat ggtacggcgg attttcaatt
      361 tttgaaagcg acattttcta ttaatccttc ttttacatat tattattttt ttcaggaatt
      421 agtagccggg ccgttttcac aaaatgcttt ggcaggttcc acaggcattt cgttttatgc
      481 tggatccatt ttgtattttc cttttgcact acaaatggat tctatcatta atgagaaatt
      541 aacttggcaa agcgcaactt ttcagttttt ggcatggaaa acatggaccg atgattatga
      601 taatcactgt aacaattgct tttggaatac aaaagatctt tatgaagtgc ttgaccatta
      661 ccttgctatt cgatttgttg atactgaaaa taataatcac tatggctgga tcagatgcga
      721 tgtaaaagag gatggcagta tacttatttt aaaagattat gcgtatgaaa cagaaccaga
      781 ttatcccatc ttaacgggcg ccaaaacaac ttatcaggaa attaccccgg gaaatattga
      841 aggggagata tatgcatctg ggaacactgt tcacataagt gttccccgtc gtccaacctc
      901 tgaatatacc gttcagattt ataatatgtc gggacaatta gtggtagctt cgcaatttga
      961 tagcccattt gttgaaatac aaatagatgt tccacgcggc atatatatag ttagggtgac
     1021 ctcagcgaat gagcaattaa gtaaagaagt tttaatagaa taaatcttag aaccactggt
     1081 tataacaaaa gctttgcaaa atggcgggtg aatactacct aagaaaattt aaccacacca
     1141 ttaattattt cagactatga actatatcgt ttagttgccg ccacttcgca aagcttcaaa
     1201 cgttataacc aatggttcag aaaaattacc ttcaaattgc acatgaagtc aattatgctc
     1261 agcgattagt cgactggctt aataaacgca ggcgacgacg tgggttgatt ttggaatttt
     1321 acgcgccaca tcgtctctaa tccctttctt ttcttaactt taaatcaggt aaaatgaaaa
     1381 agtctattat acataaaata cgttttctaa atgtcacgct actttttata atattaatta
     1441 ctggaataac attagcagca agatttgcgg gtgataatat ccgttacaat acatcagaat
     1501 catctactac cacatttatt cccccattac ccgactcaac atatatcgat tcaacagatt
     1561 tttctgggat atatgataca gcgtacacct attctgatgg tggcgattct gtttatgtcg
     1621 ttgcagattc agtttttacc gattcaatct tttcagatac ctctttgact gaagggcggt
     1681 tcggaagttc aggtaacttc atcagaaatg caattaatta ttcttcagca aaagtcggct
     1741 ccggaaccgg gcctattgtt tgcgagccac tatttgatgg cagctctcct gaattgatca
     1801 ataaaggact gtacgggata aatgtatcag gtatgtttga taatgcaacc tgcccgaacg
     1861 atggcagcgc aacagaccaa tgggattggc ttagcgacct tcggcctgaa gtactgcgtt
     1921 tcccacatgg gtcaaatgga aaatttatgc atttgttgca cgatgtttca accggtgata
     1981 cagcggccgg ttatggttat gatattgccg aacttatacg atattttgat gcttccaata
     2041 atgacaatac tgatctggtg ccgggcatat atccggatat ggctactgct ttggcaacca
     2101 tttctacaca aagcgaagcg aaccttgccg aatggaatac ctggatggat aatacagttt
     2161 cgggccaatt cggaaagttt cttgccaaat ggcaggaaca acagcaactt gttacaacag
     2221 ctacagatca ccgcaattac ttaaccgact ttattaagct gatcaggcag attgaaacgg
     2281 ataatccggg gcatacggta aaaattattg tttgcctgaa tatactgagc gaaacatcag
     2341 cgaaatgttc ggccatagtt caatttcttc gttccaatcc gatccacaat tgtacggttg
     2401 ttggcgtaga actcggcaat gaatgccctt ctgattttca ttggaaagtt atgggatttg
     2461 cgcatttttc agaacatgac actcctgatg gttgcgatga agcttttaat gggtcctatt
     2521 ggagttttat taatggtgaa gcgtatgaaa ccggagatgc acaggatcgc ctcgatctat
     2581 ttctgaatcc tgccatgcaa ctccaagatg cagcaggaca ctattataac cgtgatttta
     2641 taaaggcatt taaatataat tttcttacat catgcaaaat cggaataccc ggcgaaggca
     2701 ctcccaatga tgaactttta cctccaccgt tttttttatt gaccggctgc ccgagagggg
     2761 caacggaatg gaatacagac cttacttccc aatatggcga ggaaattgcc tctatgcccc
     2821 cggggttggc aaagaaaaaa tttgacgcgg ttattctgca tatttatctt gatgataaac
     2881 aaaattgggg gccaccaatt gcggaccttt tattaccata tccatattct gaaagatggg
     2941 actatgcttc ttttgatacc agactacaag cagcattcaa tggtattttg aattttgatc
     3001 cggcagaccc aggtagccct gcctctgaca gctttatggg ttttatcaaa tacggtcata
     3061 aaaccgcctg ggatgattat aaaacagtat tcaatttcac cccgacagcc ccaaccgata
     3121 aactgcggaa agaattatgg atgactgaat acaatatcaa gacctcggtt gatgaggata
     3181 atcttcagcg cggcgttttt acaaatggtc ttacacatgg ctttgttttg caggaatggt
     3241 ttttaaagaa tctgaaagta aattttgcat ccggttacaa gaaaaacttc ttcacgtatg
     3301 caacctatca aaactatgca ggtgttggtg aagatttgat taccccggca gacagaccgc
     3361 gggagttgta taattactta tgtaaagact attcccccta caaccttggt ggcggcaatc
     3421 ccaatcaaag agattattac atgaagcgaa caagttactt tatcatgtcc ttgttgaaag
     3481 aaatttcagc gcaggatttg aaatatgtgc gaaccagata tgccactatt ctgagtaata
     3541 tgaacactat gcccaccatg tttattgatc cgggaaacga tttcctgtat atgttttata
     3601 ccaatatgaa atcaacagag caggactata tcatcaacgg cgaaaacctg gcagcattgt
     3661 tcacaccgac acccattatt tcctttggaa cagcaactat aaaaaacata catgctttgc
     3721 agttatattc cggatcaggc aacagcactc tttttaaccc tttgttcaac acaggatata
     3781 ccaacccgac gcctgcagac cgcacctgtt atgagggaga gttgctaccg cctgcgtatc
     3841 tgtatccgat agaaattcag acaacttcct tctccgtcaa tgcacctgat tgtgcc
//
LOCUS       DAOQOU010000236         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_15813_length_3887_cov_6.191545, whole genome shotgun sequence.
ACCESSION   DAOQOU010000236 DAOQOU010000000
VERSION     DAOQOU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15813_length_3887_cov_6.191545"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLM90_13765"
     CDS             <1..206
                     /locus_tag="PLM90_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ90499.1"
                     /translation="LHPKLAAALEDNLAAKKVFDKMSPSHQKEIIRYINSLKSEASVE
                     RNVARSIQFMLGKERFVGRDPEK"
     gene            complement(207..1739)
                     /locus_tag="PLM90_13770"
     CDS             complement(207..1739)
                     /locus_tag="PLM90_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90500.1"
                     /translation="MAVSHCYTLIHALTMSEKRQFVAFSERHVVGEEKKYLALFHILE
                     SMPTYNEHQVQKAMREKGLNTKWFKGDQNYLYNQLLEHLAQFHNGKSGNLQVKAGLMH
                     IEILFDKGLFRQCRKEIEKAMALARMLEFFELQIQILFWERKIALATSDKTSRTIALI
                     LGDLEQATAMITVGNQYLLLFEKAESLRQRVIKTRKPADLAAFRKLLKDPLLGARSHP
                     PTKQSQIRFHQIHAMWAYLHKAKDDELAHNRAIIKLFDRSPEYRNEYPLDYIAIYSRI
                     LSILKLDDAKVFHAQLTNFRAFEPDIHSIGYRNIRAQIFAQSYMIELSAYLTRGYFSD
                     AMRTVRAIEASMPEHAPFISKGNSLSFQFMISYALFANGEYKSARSALNQLLNEYDDK
                     VRPDLYNFARLLSLMTHLELGNYSNIRSQASSIAYYFRKHGKLYATENKILNYFNNPE
                     HYKGRRQLHYLELIEAQLEKIRSASIEKYALGYIDFFTWIASRRERVPMSTIAGRRNK
                     RG"
     gene            complement(1847..3220)
                     /locus_tag="PLM90_13775"
     CDS             complement(1847..3220)
                     /locus_tag="PLM90_13775"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HMZ90501.1"
                     /translation="MKKYFALSVPCIMLMSIAHAQLNPSITSWLQNTTETGSYYVEGN
                     YTPIDNGILYNCQTVEYSTDYVYVHTKGIPSYPTGPFMDGNPSIAEDQDIIFKIPLHP
                     QQNTGTPTPTTPGNIGVFINGVALFDYRDGVAWNTTTSALCGGPGNPPCPGGPGAIMD
                     WNRDAVPAEKIGFDCSKGHPAMGNYHHHQNPSAFKLDLNVISTVCNLYDADGLYMIDS
                     NAHSPLIGFAYDGYPIYGAYGYKNADGTGGIVRIKSSYQLRSITERTHAADGSNVDDG
                     PDVSATYPLGYFREDYEFIAHPGEQDYLDDHNGRFCVTPEYPEGTYAYFATVDENWNS
                     AYPYVVGPSFYGVYANRAVTSVTEATTVYTPTVAISDNNFYNMHIGIYPNPTADLLII
                     QAAGITSGDLIITLYDLNGRQLAQTQIAQGQTITYFDTKTLYAGNYVVHITNGNSSQT
                     EQVTIVK"
     gene            complement(3226..>3887)
                     /locus_tag="PLM90_13780"
     CDS             complement(3226..>3887)
                     /locus_tag="PLM90_13780"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMZ90502.1"
                     /translation="APINNVIEMDMSTADLVSRLQKSAFYRKLFFQAFNDSVITGQHL
                     LQALGAFQVTLISDHSKYDSVQLGTAQFTEQEAAGYQLFKQYCNSCHTEPLFTNEGFA
                     NNGLAPDPQLHDAGRYRITGNPSDSLKFAIPSLRNLSYTYPYMHDGRFAQLRQVLDHY
                     TSEKYFSATLDPSLNHAFPLSSNDKVDMIAFLLTLDDKQFVFNKAYQFPPELLPNSKT
                     R"
BASE COUNT         1088 a          822 c          827 g         1150 t
ORIGIN      
        1 cacttcatcc aaaattagct gcagcgctcg aagacaattt agcagcgaaa aaggtgtttg
       61 ataaaatgag tccttcccat caaaaagaga tcatacgcta tatcaattct ttgaaaagcg
      121 aagcaagtgt tgaacgaaat gttgcgcgtt cgatccaatt catgctcggc aaagagcgtt
      181 ttgtaggtcg cgatccggaa aaatgatcaa ccgcgtttgt tgcgccttcc cgctatggtc
      241 gacatcggta ctctctccct gcgcgatgcg atccaagtga agaaatcaat atatcccaac
      301 gcatattttt ctatcgaagc actgcgtatt ttttctaatt gtgcttctat caattctaaa
      361 tagtgtaatt gccttctacc cttataatgc tccggattgt tgaagtaatt caatatttta
      421 ttttcagtgg catatagctt gccatgtttc ctgaaatagt aggctatgga ggatgcctgc
      481 gagcggatat tggaataatt gcccagctcc aaatgcgtca tcagacttaa taaccgggcg
      541 aaattataca agtccggtcg caccttgtcg tcatattcgt tgagcaactg attcaatgcg
      601 ctgcgagcgg atttgtattc gccgtttgca aacagtgcat agctgatcat aaactgaaac
      661 gataaactgt tgcctttact gataaatggg gcatgctcag gcatggatgc ttcgatcgct
      721 cttacagtgc gcatggcatc agaaaaatat cctcgcgtca ggtacgccga taattcgatc
      781 atataggatt gcgcgaatat ttgagcgcga atgtttcgat atccgatact atgtatatcg
      841 ggctcaaaag cgcgaaaatt tgtgagttgt gcatggaata ccttcgcatc atcaagcttc
      901 aagatcgaca gtatgcgcga gtagatagcg atataatcca gcggatattc attcctgtat
      961 tccggcgatc gatcaaataa tttgataata gcacgattat gtgcgagttc atcatccttg
     1021 gccttatgca gatatgccca catggcatgg atttgatgaa agcggatctg tgattgtttt
     1081 gtagggggat gtgaccgcgc acccagcaaa ggatctttca gtaattttct gaacgctgca
     1141 agatcagcag gttttctggt cttgatgaca cgctgacgca gtgattcggc tttttcaaat
     1201 aataggagat actgatttcc aacggttatc atcgcagtag cctgctccaa atctccgagt
     1261 atcagagcaa tcgttctgct tgttttatct gaagtagcca atgcaatttt gcgttcccaa
     1321 aaaagtattt ggatttgcaa ttcaaaaaat tccaacatcc gtgctagcgc cattgccttc
     1381 tcgatctcct tccgacactg gcgaaacaat cctttgtcga acaatatctc gatatgcatc
     1441 aatcctgctt tcacctgcag gttgcccgat ttgccattgt gaaattgagc gaggtgctcg
     1501 agcaattgat tgtataaata attctgatcg cctttgaacc atttggtatt gagccctttc
     1561 tcgcgcatgg ccttctgaac ttgatgttca ttgtaggttg gcatggattc caaaatatgg
     1621 aataacgcta aatacttttt ctcctccccg acaacatgcc gttccgagaa agctacaaat
     1681 tgtcgcttct ctgacatggt gagtgcatgt atcaatgtat aacaatgact tactgccata
     1741 aaagggctaa atcagacttc ctaaaaatag gcaaaaaact acaaatgctc accagacaac
     1801 tatgttgcag tccgatgagc atttgtaagc ttatcatgaa acattcctac tttacaatcg
     1861 tcacctgttc agtttggctg ctgttgccat tggtgatgtg cacaacataa tttcccgcat
     1921 acaatgtctt tgtatcaaaa taggttatcg tttgcccttg agctatttgc gtttgtgcca
     1981 actgtctgcc atttaaatca tagagcgtaa taattaaatc gccggaagta ataccagcag
     2041 cttgtatgat gagtaaatct gctgtcggat ttggataaat gccgatatgc atattgtaaa
     2101 aattattatc gctgatggca actgttggcg tataaactgt cgttgcctcc gtaactgaag
     2161 taacggctct gttcgcatac acaccataaa agcttggccc tacaacatat ggataagcgg
     2221 aattccaatt ctcatcaaca gtagcaaaat atgcataagt gccctcagga tattcgggtg
     2281 ttacacaaaa acgaccgttg tgatcatcaa gataatcttg ttcacccgga tgagcaatga
     2341 attcatagtc ctcacgaaaa tatcctaaag gatatgtagc acttacatcc ggaccatcat
     2401 ctacattact accatcggcg gcatgtgtgc gttctgtaat actgcgtaat tggtaacttg
     2461 atttgatgcg cacgatccca cctgtaccat ccgcattttt ataaccataa gctccataga
     2521 tgggataacc atcatatgca aatcctatca atggcgaatg tgcattgctg tcgatcatat
     2581 ataaaccatc tgcatcatat aaattacaaa ctgtcgagat cacattcagg tctaatttaa
     2641 atgctgatgg attttgatgg tgatgataat ttcccatcgc aggatgcccc ttgctgcaat
     2701 caaatcctat cttctctgca ggcactgcgt cgcgattcca gtccatgata gcaccgggac
     2761 ctccaggaca aggcggattt cccggaccac cgcatagtgc tgatgtggtc gtattccatg
     2821 caacgccatc gcgataatcg aacaaggcaa caccattgat gaacacgcca atgttgccgg
     2881 gtgttgttgg tgttggtgtg cccgtgtttt gttgtggatg cagtggtatc ttgaaaatga
     2941 tatcctgatc ttcagcaatt gaaggattgc catccatgaa tggtccggta gggtaggatg
     3001 ggatgccttt tgtgtgcaca tacacataat cggtagaata ttccaccgtc tgacaattat
     3061 ataaaatgcc attgtcaata ggtgtgtaat tgccttctac ataatatgat ccggtttccg
     3121 ttgtgttttg caaccaggaa gtaatggaag gatttaattg tgcatgtgct atgctcatca
     3181 acataatgca aggtacactc aaggcaaaat attttttcat tgtggttatc gggttttgga
     3241 attgggtaat aattcaggtg gaaattgata ggctttattg aaaacaaatt gcttgtcgtc
     3301 aagggtcagt aagaacgcta tcatatcaac cttgtcgttt gacgaaagcg gaaacgcatg
     3361 gtttaacgac ggatccaaag ttgctgaaaa atacttttcc gaggtatagt gatctagcac
     3421 ttgcctgagc tgtgcgaatc tgccatcatg catgtatgga taggtgtagc tgagattgcg
     3481 aaggctggga atggcaaatt tcaggctatc actcggattg cctgttatcc gataacgacc
     3541 ggcatcatgt aattgtggat ccggcgcaag cccgttgttg gcaaaccctt cattggtaaa
     3601 aagtggttcc gtatgacagc tgttgcaata ttgtttgaac aattgatatc ctgccgcttc
     3661 ctgttcggta aactgcgctg tgccaagttg cacactatca tatttcgaat gatcggaaat
     3721 caaggttacc tgaaaagcac ccaaagcttg taataaatgc tgaccggtga tgacagaatc
     3781 gttaaaagcc tgaaaaaata attttctata aaaagcactt ttttgtaatc gtgaaacaag
     3841 atctgctgtt gacatatcca tttcaattac attattgatc ggcgcca
//
LOCUS       DAOQOU010000237         3857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_16000_length_3857_cov_7.393740, whole genome shotgun sequence.
ACCESSION   DAOQOU010000237 DAOQOU010000000
VERSION     DAOQOU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16000_length_3857_cov_7.393740"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PLM90_13785"
     CDS             <1..1241
                     /locus_tag="PLM90_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90503.1"
                     /translation="NGDTDDGLEIKCNTFEEGKYDISLISGATTATMKKNQGACGTLT
                     SPSSNLLTDPSFSDYNTHIKTSTDINTANFYKYSFHEDEDVFDSPFAAPYNVSASIDP
                     KPDGIGGTGCDDFNLLDDNLEFQDYCPTNFTSGSGGSGRMAAPKPTKSRPPSLMMPLT
                     YNPYEYLKPAGVAYEGFLKNIRFNIEASYYLQNELIDSLNILLNEYESPLATSIKVQL
                     LEDSSAYVNAELLSAELIESPDAELTGYNSSILSIGLSLSRDTLTWLQTTEEQISTIK
                     TEAQRNDREGIQAKLLMELVDGSAYVEPINVLNEELTTEELRLIQQNIPQSEPNSIQI
                     YPHPVGSGSVVEINLGSIQSNTRFIITDAQGRYVLQFPLDNLVSYIKLDNSMLKPGVY
                     IGYVSGNLGEQISKQIIVIQ"
     gene            1246..2799
                     /locus_tag="PLM90_13790"
     CDS             1246..2799
                     /locus_tag="PLM90_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90504.1"
                     /translation="MIKACLTFWMAIVSLITLGQTTFSKLYWNDYTISGLGIATDGEF
                     VYSTGLGLYNDDELYLPNVFFLKFDVLGNQIDKTYLIAGENAYYRSGYAGGFELLNDS
                     ILFASGHFSEYDTMFPPWNRKHLGQLYAFNTEGDTLFTRKYKGSGFIEFNRLHLFNDK
                     VICGGFTQDTNGVASNAYLTKVNYEGIQIWELMLGDTIHSEATGLLDTISNSTIIGGG
                     AVVNSISPDGIMRNGKIYKLDASGNVYFSKEIGTPYDDSEIEVKVSKNGQSYLVRQYM
                     DTVINEGDYEYVQYIGKTDTNVNFIWRTFLNDKYYKYIYTLRTFEDGSIVAVGTTIVD
                     ETYEPHGYIVKLDSNGAVLWERDYTNNPEGNHYLYDFQKMPDGGYICSGLGADTIDGV
                     IQSMCWLLRLDSMGCLIPGCDEVSIPEIAVNSETYLSIYPNPFGDAATVEVHVPQDFT
                     INGTQALRLDIYDMQGRLIDGYSNIYPSNPGETIRFKMYRNTMTAGNYLATLKYGDEV
                     LNTIQFVLK"
     gene            2810..2953
                     /locus_tag="PLM90_13795"
                     /pseudo
     CDS             2810..2953
                     /locus_tag="PLM90_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
     gene            complement(3011..3556)
                     /locus_tag="PLM90_13800"
     CDS             complement(3011..3556)
                     /locus_tag="PLM90_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90505.1"
                     /translation="MDSSGKLKMRGLGIEKALTITLEANPARFKELLKRNISEGWNKW
                     SLIPGRNKAPDYLCIMEGFMDNDQFEVKPFGGGMRKRNGRDAIRFKGKIRESGNTVTL
                     HITESISPNYLAFIGPIIMVIFLYSQGSSLSIMGLMTVYAILVLLSSIFYYISGIMNI
                     ADFRHQFNKEVSYLARLSLKE"
     gene            3650..>3857
                     /locus_tag="PLM90_13805"
     CDS             3650..>3857
                     /locus_tag="PLM90_13805"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90506.1"
                     /translation="MKHILGILPILSVLSCTAQQPNATATDLGVMFYNQENLYDTIDD
                     PKVDDAIFLPDAERQWNTEKYNKKL"
BASE COUNT         1214 a          686 c          802 g         1155 t
ORIGIN      
        1 acaatggcga cactgacgat ggtctggaga tcaagtgcaa tactttcgaa gaagggaaat
       61 atgatatttc attgatttcc ggtgctacaa cagcaacgat gaaaaaaaat cagggagctt
      121 gtggaacatt aacttcgccc tcaagcaacc ttcttacaga cccaagcttt tctgattaca
      181 atactcatat taaaacatca actgacatta atacagccaa tttttataaa tattcattcc
      241 atgaggatga agatgttttt gattctccgt ttgctgcacc ttataatgtt tctgcatcta
      301 ttgaccccaa acctgatgga attggcggca ctggatgcga tgactttaat cttttagatg
      361 ataatttaga atttcaagat tattgtccga ctaacttcac ttccggtagc ggcggcagtg
      421 gacgcatggc ggcacctaaa ccaacgaaaa gccgcccgcc aagtctgatg atgccactca
      481 catacaaccc atacgaatat ttgaagccgg ctggagttgc ttatgaaggt tttttgaaaa
      541 atattcgttt taatatagaa gcctcttatt atttgcaaaa tgaactcata gatagcctta
      601 acatattact gaatgaatat gaaagtcctc tggcaacaag catcaaggtg caattattag
      661 aggattcaag tgcctatgtt aatgcagaat tacttagtgc tgaactaatt gaatctcccg
      721 atgctgaact gaccggatac aattcaagta tattgtctat tggcttgagc ctatctagag
      781 atacattaac gtggttgcaa acaactgaag aacaaatttc aactattaaa acggaagccc
      841 aacgaaatga ccgagaaggc atacaagcga agttgctcat ggagttagtt gacggaagcg
      901 catacgtaga accgataaat gtgttaaacg aagaattgac gaccgaggag ttgaggttga
      961 ttcaacaaaa cattccgcaa agtgaaccga attcaataca aatatatccg catccggttg
     1021 gcagcggcag tgttgtcgaa ataaatcttg ggtcaattca aagtaatact cgatttatta
     1081 tcacagacgc acaaggtaga tacgtattgc agttcccact cgataatttg gtttcctaca
     1141 ttaagttaga taattctatg ctgaagcctg gtgtgtatat tggttacgta tctggtaatt
     1201 taggagagca aataagtaag caaattattg ttatacagta atcgtatgat aaaagcttgt
     1261 ctgacatttt ggatggcaat tgttagtttg attactttag ggcaaacgac attctccaaa
     1321 ttgtattgga acgactatac tattagtgga ttagggattg caacagatgg cgagtttgtg
     1381 tattcaacag ggttgggact ttacaatgac gatgaattgt atcttccaaa tgtttttttc
     1441 ttaaagtttg atgtcttagg taatcaaatc gataaaacct atttaattgc aggagagaac
     1501 gcctattata gaagtggata tgcgggtgga ttcgaattgt taaatgatag tatcttattt
     1561 gcttcaggcc actttagtga gtatgatact atgtttccac cgtggaacag gaaacacttg
     1621 ggacaactat atgcatttaa tacagaaggt gatacgttgt ttactagaaa atataaagga
     1681 agcggattca ttgaatttaa tcggctgcac ctgtttaatg ataaagttat ttgcggcgga
     1741 ttcacgcagg atacaaatgg tgttgcaagc aatgcgtatt tgactaaagt aaattacgaa
     1801 ggtattcaaa tttgggaact gatgctaggt gatactattc attctgaagc tacaggtcta
     1861 ctagatacca tatcaaacag cacaataatc ggtggaggcg ctgtcgtaaa ttctatcagc
     1921 ccagatggaa ttatgcgcaa tggtaaaatc tacaaattgg atgcatcggg aaatgtctat
     1981 ttctccaagg aaatcggtac cccatatgat gattctgaga tagaggtaaa agtgtcgaag
     2041 aatggtcagt cgtatttagt gcgacaatat atggacacag tgattaacga aggagattat
     2101 gaatatgtgc agtatatagg caaaacggat acaaatgtaa acttcatttg gcgtactttt
     2161 ttaaatgata aatattacaa atatatttat acccttcgca catttgaaga tggaagcatt
     2221 gttgctgttg gaacaactat agtagatgaa acttatgagc cacatggcta tatagttaaa
     2281 ttagacagta atggcgcagt tttatgggag cgggattata caaataaccc cgagggcaac
     2341 cattatctat atgattttca aaaaatgcct gatggaggat atatatgctc tggtttggga
     2401 gcagatacta tagacggtgt aatacaatcg atgtgttggc ttttaagatt ggacagcatg
     2461 ggttgcctca ttccgggttg tgatgaggtg tcgatacctg aaatagctgt taattctgag
     2521 acttatctta gtatatatcc taacccgttt ggagatgcag caacagtaga agtccatgtg
     2581 cctcaagatt ttaccataaa cggcacccag gcactcagac ttgatattta tgatatgcaa
     2641 ggcagattga tagatggata cagtaatatt tatccaagca accccggaga aaccatccgt
     2701 tttaaaatgt atcgcaatac aatgacggct ggaaattacc tcgcaacgct taagtatggg
     2761 gatgaagttt taaacacaat tcaatttgtg ctaaagtgat tgattttttt tgtatctcgc
     2821 cctcagctac gatcaccgca tcatcgacgg caaagaaagt gtaagcttct tagttcgcgt
     2881 aaaagagcaa cttgaaaagc ctgagcaact attgtttgga attgatccgg tggggttgtt
     2941 gttggagatg tgaattcaaa tacgatttct gtttgtaaac gaatgactat gcatttgttg
     3001 taaaagcaga ttactccttc aaactcaacc ttgccaaata agaaacctcc ttattaaatt
     3061 gatgtctgaa atctgcaata ttcataatac ctgatatgta ataaaagata gaggataata
     3121 aaacaagaat tgcataaaca gtcattaagc ccataatgga gagtgacgac ccctgtgaat
     3181 ataaaaatat gaccataatg atggggccta tgaatgctaa gtaatttgga ctaattgatt
     3241 cagtgatgtg cagggtaact gtattacctg attctcttat tttcccttta aaccgaatcg
     3301 catctctgcc atttcttttt cgcattccgc caccgaaagg tttaacttca aattggtcgt
     3361 tgtccatgaa gccttccatg atgcataaat aatccggtgc cttgttcctg ccgggaatca
     3421 gcgaccattt gttccatcct tcgctgatat ttcgttttag cagttcttta aaacgtgcag
     3481 gattggcttc cagtgttatg gtgagggctt tttctattcc caaaccacgc attttcaact
     3541 tgcctgagga atccataagg gtaaatttaa ctaaaagcac gttgccttca aattggcata
     3601 gggtttggat ttatccgcta cctttgtcac ctgtaaaaga cgaccggcaa tgaaacatat
     3661 tttagggata ctaccgattt tgtctgtgct ctcctgtaca gcgcagcaac cgaatgctac
     3721 tgctaccgac cttggtgtga tgttttataa tcaggagaat ctgtatgata ccatcgatga
     3781 tccgaaagtg gatgacgcta tttttttacc tgatgctgaa cgccagtgga ataccgagaa
     3841 atacaataag aaactct
//
LOCUS       DAOQOU010000238         3844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_16107_length_3844_cov_4.370810, whole genome shotgun sequence.
ACCESSION   DAOQOU010000238 DAOQOU010000000
VERSION     DAOQOU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16107_length_3844_cov_4.370810"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLM90_13810"
     CDS             complement(<1..727)
                     /locus_tag="PLM90_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90507.1"
                     /translation="MRRLFALAILLNLILMVKPLSACDQCGCSSGATFNNLSAFATYN
                     FIMLRLGYSSMQGYGDVGSFALNTNADIVGGYSLNTRLHISAFLPVKYNYFEHDQNSH
                     TTLGIGDAGFMANYILYSNSDKMMKRVSSTLSIRGGIELPTGNFNENFRADDVPAAIS
                     TGSGSFDFFWGLRYTIRSGNNAITADYIGKYNTENAVAYQFGFQQSANVYVTRSIKKT
                     NAAWLPYAGVSGEFVSGDTYHDNF"
     gene            complement(730..1131)
                     /locus_tag="PLM90_13815"
     CDS             complement(730..1131)
                     /locus_tag="PLM90_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90508.1"
                     /translation="MKFNTAIVLACTIFAISATSCKQDEVPEVTYDVTFTIIEPEADF
                     VAVSGDELHMEVDLQGTKAIGNAEMLLIDEMSGDTLFTYSTTTTSDFLTMHEHYIPSV
                     TMSTMCHFVVSAWESNYADRLTEEVHLTINP"
     gene            complement(1190..1564)
                     /locus_tag="PLM90_13820"
     CDS             complement(1190..1564)
                     /locus_tag="PLM90_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90509.1"
                     /translation="MKRIVIIILTFAYLSGICSPFYTYVGYLINKDYIAANLCENKDK
                     PEMNCEGKCQLTVQLAKDVEQKQDNKAPQSGNRELNPHTIASFNFTPYIGVISVFAEN
                     RSQQQFADMYIAAPESPPRLFV"
     gene            complement(1685..2236)
                     /locus_tag="PLM90_13825"
     CDS             complement(1685..2236)
                     /locus_tag="PLM90_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HMZ90510.1"
                     /translation="MKKAAFYLLLVTFSFSASMAYAQTTTVGGEAMYPQKDIVDNAVN
                     SKDHTTLVAAVKAAGLVETLKSAGPFTVFAPVNDAFENLPDGTVATLLKPENKDMLTK
                     VLTYHVVAGKLDYAAIEKMIKDGKGTAELTTVSGGKLWAMMNGKHNIQLKDESGNLIN
                     ISTYDVYQSNGVIHVIDHVALPK"
     gene            complement(2386..3048)
                     /gene="phoU"
                     /locus_tag="PLM90_13830"
     CDS             complement(2386..3048)
                     /gene="phoU"
                     /locus_tag="PLM90_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HMZ90511.1"
                     /translation="MSQIDNPIHELKHSLEQMFQLVSTQLEKSKEALSKFDNDLAREV
                     IQIEKRVNAQELQIDRVAENFLALYSPVAIDLRFVLAVLKINNNLERIGDIAEGISKY
                     LNETERPLDAKLMEESNVIPMYEAAVRMLSDVHEAFEKEDTTIARKIFKSDDYLDHIN
                     LEAINNLTEAMAGDASKIQDALYLISIIRKLERIGDQLKNIAEEIIFYIEAKVLKHAK
                     QK"
     gene            complement(3078..3833)
                     /gene="pstB"
                     /locus_tag="PLM90_13835"
     CDS             complement(3078..3833)
                     /gene="pstB"
                     /locus_tag="PLM90_13835"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HMZ90512.1"
                     /translation="MSNKLTAHEVHVWYGSFHALKNISLTIKPNTVTAFIGPSGCGKS
                     TFLRLFNRMNDYIDGFHMGGEITIDGRNIYGKRVMVEELRKEVGMVFQKPNPFPKSIY
                     DNVVYGLRIQGINDKHELEEACETSLRHAALWDEVKDNLKKSALALSGGQQQRLCIAR
                     TLAVKPTMILMDEPASALDPISTAKIEELILQLKDDYTIAIVTHNMQQAARISDKTAF
                     FYLGELIEYDKTVNIFTNPKEEKTQSYITGRFG"
BASE COUNT         1109 a          807 c          750 g         1178 t
ORIGIN      
        1 taaaattatc gtgataagta tcgccgctga caaactcgcc tgaaacaccg gcatagggca
       61 gccaggccgc attcgttttt ttaatcgatc tcgttacata tacatttgct gattgttgaa
      121 atccaaattg ataggcaaca gcattttcag tattgtattt accgatataa tctgcagtga
      181 tggcattatt gccacttctg atggtatagc gcaatcccca aaaaaaatca aaacttccgg
      241 agccggtgga gatagcagca ggaacatcat cagcgcgaaa gttctcatta aaatttcctg
      301 ttggaagttc tattcctccg cgtatactca gtgttgatga tactcttttc atcatcttat
      361 cactattcga atacaatata taattagcca taaaaccggc atccccaatt cctaaagtgg
      421 tatgactatt ttgatcatgc tcaaaataat tatacttcac aggcaggaac gcactaatat
      481 gcaatcgcgt attcaagcta tagccaccaa caatatcggc attggtattc aatgcaaagg
      541 agcctacatc accataacct tgcatactgc tatagccaag tctcagcata ataaaattgt
      601 aagtggcgaa tgcactgagg ttattaaaag tggcgccact gctgcatccg cactgatcgc
      661 atgcagacag tggtttcacc attaatatca gattcaataa tatagccaat gcgaaaaggc
      721 gtctcataat catggattga tagtgagatg cacttcttca gttaacctgt cggcataatt
      781 actttcccat gcagaaacca caaagtgaca catagtagac atggtaacag acggaatata
      841 atgctcatgc atggtcagga aatctgacgt agtggtggtt gaataagtaa acaaagtatc
      901 gcctgacatt tcatctatga gtaacatctc ggcattgcca attgcttttg ttccttgcaa
      961 gtcgacttcc atgtgcaatt catcacccga cacggcaaca aaatcggctt caggttcaat
     1021 aatggtgaat gttacatcat aagtaacttc aggaacttca tcttgtttac atgaagtagc
     1081 agaaatagcg aatatggtac aagctaatac aatagcagta ttaaatttca taaaaattta
     1141 gtttgtgcaa ttaaataata aattcaagtg cgttggcaca gggttgctat tacacaaaca
     1201 aacgtggagg agactccggc gcggcgatat acatatcggc aaactgctgt tgcgaacggt
     1261 tttccgcaaa aactgaaatt acgcctatat aaggagtaaa attaaacgat gcgatggtgt
     1321 gaggatttaa ctcacgattg cctgattgag gcgctttatt atcctgcttc tgctcaacat
     1381 cttttgccaa ctgcacggta agctgacact ttccttcgca gttcatttcc ggcttatcct
     1441 tgttttcaca aaggtttgca gcgatataat ctttattgat gaggtaacct acatacgtat
     1501 aaaatggcga gcagatgccc gacaagtatg caaaggtcaa tatgatgata acaatgcgtt
     1561 tcacctcagg gtagtagtgc aaagatacat actcacctat tcaggtttat gactttggtc
     1621 accatttaaa aaaaattagg gatcgaccct caggccgacc cctaacgtta caaaacatca
     1681 gaacttactt aggaagtgcc acatggtcga tcacatggat cacgccattg ctttgataca
     1741 catcgtaggt gctgatattg atgaggttac cgctctcatc tttcaattga atattgtgct
     1801 taccattcat catggcccaa agtttaccgc cacttacagt agtaagctca gctgttcctt
     1861 tgccatcctt gatcattttt tcaattgctg cataatccaa tttacctgct acaacatggt
     1921 aagtgagcac ttttgttagc atgtctttgt tttcaggttt tagcagggtt gctacggtgc
     1981 cgtcggggag attttcgaag gcatcattta ccggtgcgaa aaccgtaaat gggcctgccg
     2041 actttaacgt ctcaaccaac cctgctgctt tcacagctgc taccagtgta gtatggtctt
     2101 tagaattaac ggcattatct actatgtctt tttgaggata catggcttca ccgccaacag
     2161 tagtggtttg cgcatatgcc attgaagcgc taaaagaaaa ggttaccaat aaaaggtaaa
     2221 atgctgcttt tttcatgatt ttagtttttg tatgcagcta tttacgcatg acagttcacg
     2281 catggtttta aaaaatcaaa aaaaatattt ttcaaaaaac gatgcgtata aaaaaccaaa
     2341 acccggctac tttcgtaacc gggtttcttg aatccgaatc gttaattatt tctgtttcgc
     2401 gtgcttgagc acttttgctt ctatgtaaaa tatgatctct tcagcaatat ttttcaattg
     2461 atcgccgata cgttccagtt tacggatgat actgatgaga tacaaggcat cctgtatttt
     2521 agatgcatct ccagccatgg cttctgtgag gttattaatg gcttcgagat taatgtgatc
     2581 gagataatcg tcgctcttga atatttttct tgcgatggta gtatcctcct tttcaaaagc
     2641 ctcatgtaca tctgataaca tgcgcactgc agcttcatac atgggaatga cattgctttc
     2701 ctccatcagt ttggcatcta aaggcctttc agtttcattc agatatttag aaatgccctc
     2761 tgcgatatcg ccgatgcgct cgaggttgtt gttgattttt agaacagcaa gcacaaatcg
     2821 caggtcaatg gctacaggag aatacaatgc gaggaaattc tcagccacac gatcgatctg
     2881 taattcctga gcatttacgc gcttttcaat ttgaatcact tcgcgcgcga ggtcattatc
     2941 aaatttagaa agagcttcct ttgacttctc caattgtgtt gaaaccaact gaaacatttg
     3001 ttccagcgag tgctttaact catgtatcgg attatctatt tgagacatag taatgaaatt
     3061 aatggtaata aaaaatatca accgaaacgg ccggtgatat agctttgagt tttttcttct
     3121 ttaggatttg tgaagatgtt aactgtttta tcgtactcta tcaattctcc caggtagaaa
     3181 aatgctgttt tgtcgctgat acgtgccgct tgttgcatat tgtgtgttac aattgcgatg
     3241 gtgtaatcat ccttcaattg caaaatcagc tcttcaattt tagcggttga gatagggtcc
     3301 aacgcacttg caggctcgtc catcaaaatc atggttggct ttactgccaa ggtgcgggca
     3361 atacacaaac gctgttgctg accgccggat aatgcaagcg cagatttctt gagattatct
     3421 ttcacttcat cccaaagcgc cgcatgtcgc aatgatgttt cgcaagcttc ttcaagttcg
     3481 tgtttatcgt tgataccttg aatgcgtaat ccgtaaacaa cattatcata aattgatttc
     3541 gggaaggggt taggcttttg gaataccata cctacttcct tgcgcagttc ttccaccatc
     3601 acgcgtttgc cataaatatt tcttccgtcg atggtgattt ctccacccat atgaaacccg
     3661 tcgatataat cattcatgcg attaaacaaa cggaggaatg ttgatttccc gcaaccggaa
     3721 ggaccaataa atgcagtgac agtatttggc tttatggtga gggaaatatt tttcagggca
     3781 tggaaacttc cataccaaac gtgtacttcg tgtgctgtta atttattact catattccac
     3841 ttac
//
LOCUS       DAOQOU010000239         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_16280_length_3818_cov_5.149880, whole genome shotgun sequence.
ACCESSION   DAOQOU010000239 DAOQOU010000000
VERSION     DAOQOU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16280_length_3818_cov_5.149880"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3818
                     /locus_tag="PLM90_13840"
     CDS             <1..>3818
                     /locus_tag="PLM90_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90513.1"
                     /translation="TTYLLPIDPTSHYIWRHVADGEYSLSNIDNAHTIRLSMTTGDDL
                     SGANFISNFEPDPELIPAGYPIVTTPIDLFTLSQNGGITFKSGYISYTNTAMAGGSYV
                     LHSFPVATCGHDAMLEQPCQEPEHFSAFNLNDLLAELLGNADPIIEDTYIALESPDIY
                     PGWTTHLSTMMGGSDVQDEFMYIQSYTGNTLDIIFKTEIATDTYVDLCHVSLTIADPL
                     APLSLEDLSANILLVPHAELAEADGIHDFTLECTDGSCTDDEIATIAWGYIDCLTLKA
                     CPCPDKNINGSFYYDVVTDVTIEGGVIGVVGDHPITGVHDAESYSIGTTEKTIMLDYQ
                     TYLKSGKIDITETAELKLQLFAFGKEALIDTIYADSTADTITVYLRLDGYDAVGSNLR
                     TGIGECSSNPIETPYIPYIDPCLQDQIDLAINNAYAAYEQQIIELTESFKEAYIAQCM
                     GNLSEDFTKSFSSSEYHYTLYYYDQAGNLTMTVPPAGVDILDATEMTAMESYRYGGGD
                     EIVPNHTLETRYRYNSLNQLTMQSTPDAGESRFWYDALGRIVLSQNAKQAHVGESGVD
                     QQEFSYTRYDELGRIYEVGELQIDDELITSTYIKAAVIDQASLTSALIAEGTVSEVTS
                     TYYNQPFTLPGSPFGTSGQTNQRNRISAVTIEAEPDTDPDTYDHATHYSYDIHGNVHT
                     LIQDNPSIGEIQAEERFKKVQYTYDLVSGNVMLVAYQDNMADQMYHAYQYDADNRIYE
                     VQSSRDGYIWEKEARYFYYRHGPLARVETGDLIVQGTDYAYTIQGWLKGVNAGALIPD
                     RDMGKDGYDDASNTHKYIAQDAFGFTLGYFDDDYTQINTGAAYKFLPNVSATNFDGAA
                     NSLFNGNIMSMSTALLRPLEAGDADGKHDKADVLGKVYSYDQLNRIKSSSSYEKAMTT
                     TAAFTWGGASTYAVNKWKTSYTYDGNGNILTMNRAGTGASGYDMDQMTYHYYEGTNQL
                     QYVGDAVADAAYTDIDIDDQAGIDSDPIATGTQHDIYTYDKIGNLVSDLSEGIESIAW
                     TVYGKIKSIAKSDDTNLEFAYDATGNRIMKKFTPASGPIVYTYYVRDAQGNIMAVYTR
                     EEEGTSNTLDLSELHIYGSSRLGMVQTTVDMLASFTEDSYFYRTLGEKRYELTNHLGN
                     VLSVISDRRLAFDTNADGVINIYEADVISAQDYDPFGMLLPGREWKEGYRFGFGGLEQ
                     DYEISSRGNYFTAEFWEYDAMLGRRWNLDPIRKTHESPYAAFSNSPLFLIDPTGADTL
                     LYSKNTGTKL"
BASE COUNT         1159 a          798 c          820 g         1041 t
ORIGIN      
        1 taaccactta cctattacct attgatccca catcacatta tatctggcgg catgttgctg
       61 atggtgaata ttcactttca aatatcgata atgctcatac cataagattg tcaatgacta
      121 ccggcgatga cctgagtggt gcaaatttca tcagcaattt tgagccagat cctgaattaa
      181 ttcctgccgg ctatccaatt gtaacaacac caatcgattt gttcacgcta tcgcaaaatg
      241 ggggcataac tttcaaatca ggttatatta gttatacgaa tacagctatg gcaggtggaa
      301 gctatgtact ccattcattt cctgtagcca cttgcggcca tgacgccatg cttgagcaac
      361 catgccagga accggagcat ttttcagcct ttaatttaaa tgatttgtta gccgaattgc
      421 ttggcaatgc tgatccgatc atcgaagata cttatatcgc tttagaatct cctgacattt
      481 atccgggctg gaccactcac cttagcacaa tgatgggtgg atcggatgtt caagatgaat
      541 tcatgtatat tcaaagctat acaggaaaca cattggatat catctttaaa actgaaattg
      601 caacagatac ttatgtagac ctttgccatg tgtcgcttac tattgctgac ccactggcgc
      661 cgctatctct ggaggatttg agtgctaata ttttattggt gcctcatgcc gaattagctg
      721 aagccgatgg cattcatgat ttcaccttgg aatgcacaga tggctcatgc accgatgacg
      781 aaattgcaac tattgcgtgg ggctacatag attgtttaac actcaaagca tgtccttgtc
      841 ctgacaaaaa cattaatggc tcattctact atgatgttgt aactgatgtc acaattgaag
      901 gcggtgttat aggtgttgtt ggcgatcacc cgatcaccgg tgtgcatgat gccgaatcat
      961 attcaattgg caccacagaa aaaacgatta tgcttgatta tcaaacatat ttgaaatcag
     1021 gcaagatcga tatcactgag actgctgaac tgaaattgca actttttgct tttgggaagg
     1081 aggctttgat cgacactatt tatgccgatt caactgccga caccataact gtttatctcc
     1141 gtcttgatgg ttacgatgca gtaggttcaa atctacgtac cggcataggc gaatgttcat
     1201 caaaccccat agaaacacca tatataccat atattgaccc ttgcctccaa gatcaaatag
     1261 atttggctat taacaacgcc tatgcagcct atgagcagca gattattgaa ttaacggagt
     1321 cattcaaaga ggcttatata gcacaatgta tgggtaattt gtctgaagac tttacaaaat
     1381 cattcagctc ttccgaatat cattatacac tttattatta tgaccaggca ggaaatctaa
     1441 ccatgacagt tcctccagcc ggagtagaca tattggacgc cactgaaatg actgcaatgg
     1501 aaagttatcg atacggcggt ggtgatgaaa ttgtgccaaa ccatacatta gaaacaagat
     1561 atcgatataa tagtctaaat cagctcacca tgcaatcaac tcctgatgcg ggtgaaagcc
     1621 gattctggta tgatgcactt ggtagaatag tcttatccca aaacgcaaaa caagcccacg
     1681 ttggtgaatc cggtgttgat caacaagaat tcagctatac acggtatgat gaactaggcc
     1741 gtatatatga agttggtgaa ttgcaaattg atgacgaatt aataacctcc acatatatca
     1801 aggcagcagt tatagatcag gcatctttga cttccgctct tatagctgaa ggaactgtat
     1861 ctgaagttac cagcacctat tacaatcaac catttacttt accaggttca ccatttggta
     1921 catccggtca aactaaccaa cgcaacagga tcagcgccgt aacaatagaa gctgaacccg
     1981 atacagatcc ggatacttat gaccatgcca cgcattatag ctacgatatt catggcaatg
     2041 tgcatacgtt gatccaggac aatccttcta ttggtgaaat tcaggctgaa gagcgattca
     2101 aaaaagtgca gtatacctat gaccttgtaa gcggaaatgt aatgctggtc gcctatcaag
     2161 ataatatggc cgatcagatg tatcatgcct atcaatatga tgccgataat cgaatttatg
     2221 aagtgcaatc ctcgcgcgac ggatacattt gggaaaaaga ggcgaggtat ttctactacc
     2281 gacacggacc tttggcgcgg gtcgaaaccg gtgacctgat cgtgcaaggt accgattatg
     2341 cctataccat tcaaggttgg ctgaaaggag taaatgccgg agcgcttatt cctgatagag
     2401 acatgggtaa ggatggatat gatgatgcct caaatacaca taaatacatc gcacaggatg
     2461 catttggatt tacattaggc tatttcgacg atgattatac ccaaataaat accggtgctg
     2521 catacaaatt ccttccaaac gtgagtgcaa ctaattttga tggtgcagca aacagcttat
     2581 tcaatggcaa catcatgtcc atgagcacag cacttttgcg tccactggaa gcaggtgatg
     2641 ccgatggcaa gcatgataag gccgatgttt taggaaaggt gtattcttac gatcaattga
     2701 atcgcattaa atccagtagc agttatgaaa aggcaatgac cacaactgct gcgtttactt
     2761 ggggcggtgc aagcacttat gctgttaaca aatggaaaac atcatatacc tacgatggta
     2821 atggaaatat cctgacaatg aaccgtgctg gaacaggcgc atcaggatat gatatggatc
     2881 aaatgaccta tcactactat gagggcacca atcaattgca atatgttggc gatgctgttg
     2941 ctgacgcagc atacacagat atagacatcg acgaccaagc cggaatagac agcgacccaa
     3001 ttgctacagg cactcagcac gatatctata cctacgataa gattggaaat ctggtatcag
     3061 accttagcga aggaattgaa tcgattgcgt ggacggttta cggaaaaatt aagtctattg
     3121 ccaaatctga tgataccaat ctggagtttg cctatgatgc aaccggtaac cgcatcatga
     3181 agaaattcac accggcaagc gggcctatcg tttatacata ctatgtgcgc gatgcccaag
     3241 gaaacatcat ggctgtatat acaagggaag aagagggcac atccaatact ttggatctga
     3301 gtgagttaca tatttatggc agtagtcgtc taggcatggt gcaaacaaca gtagacatgc
     3361 ttgcatcatt cacagaagat agttattttt acagaaccct tggtgaaaag cgttatgaat
     3421 taaccaacca ccttggaaac gttctttcag ttattagtga ccgccgctta gcttttgata
     3481 caaatgccga tggcgtcata aacatttatg aggcagatgt tatctccgct caggattacg
     3541 acccatttgg gatgctactc ccggggcgtg aatggaagga ggggtatagg tttgggtttg
     3601 gtggtttgga acaagattac gaaatatcca gccgtggaaa ttattttacg gctgagtttt
     3661 gggagtatga tgccatgtta ggtagaaggt ggaacctaga ccccataagg aaaacacatg
     3721 agtccccgta cgccgcattt tcaaatagcc cgttgtttct cattgatcct acaggagctg
     3781 acactttgtt gtattcgaaa aacactggaa ctaaactt
//
LOCUS       DAOQOU010000240         3780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_16555_length_3780_cov_5.562148, whole genome shotgun sequence.
ACCESSION   DAOQOU010000240 DAOQOU010000000
VERSION     DAOQOU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16555_length_3780_cov_5.562148"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            466..1596
                     /locus_tag="PLM90_13845"
     CDS             466..1596
                     /locus_tag="PLM90_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HMZ90514.1"
                     /translation="MALLFNENEVNCTLGVEMELQVLDAERLLLIPRGYEIIQAVNSP
                     RLAKEMFRSTLEIVTGICNNVHDVHHDFLESLEQVKSYAEKNNLRFAGTGTHPLAMYQ
                     DRITTTGKRYQDLMDRNQWLIRRMAVYGLHVHIGMHNAEECMRFNHFFLRFVPHLIAL
                     SASSPYWQCSDTGLAAARPTMYEAHPTSGIPYLATDWSDFERVYHAMIDTGSIESMKD
                     IWWDIRPSPTYGTLELRMCDGPATLLELESIVALIHVLALWFNDHGDRYFKEHRAIPD
                     RWVLRENKWRVIRNGIDAVLIDPDTLHTEKFVSAMSYWLEQVQPYAEKFGYENYMQTI
                     KQILTKGNSAQRQRDVFKNTGSLEKVVAHNVTEFEQGKPQWM"
     gene            complement(2154..2582)
                     /gene="ybeY"
                     /locus_tag="PLM90_13850"
     CDS             complement(2154..2582)
                     /gene="ybeY"
                     /locus_tag="PLM90_13850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMZ90515.1"
                     /translation="MLGIQYTSTKTLFRLKEKERITIWLEHLMAMHHKFPGNLTFIFC
                     SDEYLLEMNKQYLNHDTYTDIITFNYNEGHYISGDIFISVDRVKENAGSFKVKFEDEL
                     HRVMAHGVLHLLGFNDKTKKDEAEMRKMEEMALGMMNVGM"
     gene            complement(2609..3019)
                     /locus_tag="PLM90_13855"
     CDS             complement(2609..3019)
                     /locus_tag="PLM90_13855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ90516.1"
                     /translation="MSEQPLSKQLSLRSEPLNITLIEPFVEELRQRLHLADEVYGNML
                     VCITEAVNNAILHGNKSDATKEVKVQIRTEQNTLCCCVQDQGCGFDYNNLPDPTSPEH
                     IEEVGGRGIFLMKHLSDVVIFSNDGSSVEIQFKI"
     gene            complement(3033..>3780)
                     /locus_tag="PLM90_13860"
     CDS             complement(3033..>3780)
                     /locus_tag="PLM90_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90517.1"
                     /translation="SAVNDGLAETVEQMGDREVAKSNLYQQQVMTGYNNLALMLDEVM
                     QQMQEQMAKSMPGSQMCQKPGGSSDSQLPSMSEMQKQLNEQLNKMKGQMEQGKKPGGN
                     QGMSQELAEMAQRQAALRQALQELAEQLGGGNTEDGKLAKQLEQIADKMDQTEEDIVN
                     KRITQETLKRQEEILTRLLEAEESDRQRKQDNERQSNTANEINRTIPPAIEEYLKKRN
                     AELDLYKTVSPDLKPFYKNLVEEYLRDINQ"
BASE COUNT          996 a          924 c          765 g         1095 t
ORIGIN      
        1 cctctccatc tcctaccgtc gatagagagg gagctaattt cacttcggaa ttaaaatatt
       61 gcaaagcaat ataattgcat tccctgttaa cgcgccggcg ttaacaggcc aatcacactt
      121 gctaaccccc acacttgcta acttgctaac ccccacactt gctaacttgc taacccccac
      181 acttgctaac ttgctaaccc tcaaacttgc taattagccc gcctgcttaa attcgcccac
      241 gaaatcacat ctcattgaaa atcaattatt tgcgaacgaa aaatgtgcta aactatgtta
      301 atcccatccc caaaaattgc ctccaatcat ttcgtttatt aaattagcac aatctgatta
      361 tcggttctta tgctatttgc atatagcatt gccgaaatcg cgccccggaa ttttgcgcac
      421 cttggatttt gataattgta gttgttaacg ataaaatgaa gaagaatggc cttactattt
      481 aatgaaaatg aagtgaactg caccctcggc gtagaaatgg aattgcaagt gctggatgcc
      541 gagcgactat tactaatacc tcgaggttac gaaattattc aggcagtaaa ctcgccgcga
      601 ctggcaaaag aaatgtttcg cagcaccctc gaaattgtca caggtatttg taataatgtg
      661 catgacgtgc atcatgattt tttggagtcg cttgaacaag taaaatcgta tgcggaaaaa
      721 aataatttgc gattcgcagg aacgggcaca catccgcttg ccatgtatca ggataggatt
      781 accactaccg gaaaaagata tcaggattta atggatcgca atcaatggtt aatacgtcgc
      841 atggcagtat atggtttaca tgtacatatc ggcatgcaca atgcggaaga atgcatgcga
      901 tttaatcatt tctttcttcg atttgttcca catctgattg cactaagcgc ctcatcacca
      961 tattggcaat gttcagatac cggattagct gctgcgcgac caaccatgta tgaagcacat
     1021 cctacaagtg gtattccgta tctcgctacc gattggagtg attttgaacg cgtatatcat
     1081 gcaatgatag ataccggttc gattgaaagt atgaaagata tttggtggga tattcgacca
     1141 agtccaacat atggcacact cgaattaaga atgtgcgatg gtcctgcaac attattagag
     1201 ttggaaagta ttgttgcgct catccatgta ttagcattat ggtttaacga tcacggcgat
     1261 agatatttta aagagcacag agcaattccc gacagatggg tacttcgcga aaataaatgg
     1321 cgtgtaataa gaaatggcat tgacgccgtg ctgatagatc cggatacctt gcacacagaa
     1381 aaatttgttt cagcaatgtc ttattggctc gaacaagtac aaccctatgc cgaaaaattc
     1441 ggatacgaaa attatatgca aacaataaaa caaattctca ccaaaggaaa cagcgcgcaa
     1501 cgtcaacgcg acgttttcaa aaacaccgga tcactggaaa aagtagtcgc ccacaatgtc
     1561 acggaatttg aacagggaaa accacaatgg atgtaggttg atatgaagtt agttgtctaa
     1621 attaaatgct gaaattcttt tgtgatcgtg ttcgggtgtt caggtgcggg cgccggtgtg
     1681 gttgactgtg cgccttcgcc actttgcgcg aacccaatca cagttgctaa cttgccaacg
     1741 ctcaaacttg tagattgcga ggtagttatt atcccaatat aaattctcgc ttttttcacg
     1801 cagagtcgca gagccgcaga gataaacatg caggcattcc cacatttgcc aacttgctaa
     1861 cccccgcccc acacctcctt tcgtcggagc ggcaagctct ccatctccta ccgtcgatag
     1921 agagggagct gattgcgctt cggaattaaa atattgcaaa gcaatatcat tgcattccct
     1981 attaacgcgc cagcgttaaa agcacctcac acttgctaat ttgctaattc tcaaatttgc
     2041 taattaacca cacttgctaa cctgctaacc cccaaacttg ctcactaaga cgagcgtctt
     2101 catgcaagca cacaccccat tggctaattg tttattccca cattcccaca ttctcacatt
     2161 cccacattca tcatccccaa cgccatttcc tccatcttcc tcatttccgc ttcatctttt
     2221 tttgttttat cgttgaagcc gagcagatgc aaaaccccat gtgccatcac gcggtgtaat
     2281 tcatcttcga atttaacttt gaaggaaccg gcattttctt tcacgcgatc aacactgata
     2341 aaaatatcac cgctgatata gtgaccttcg ttgtaattaa atgtgatgat atcggtatac
     2401 gtatcatgat tgagatattg tttattcatc tcaagcagat attcgtcgct gcaaaaaata
     2461 aatgtgagat tgcccggaaa tttatgatgc atcgccatta aatgttcgag ccaaatagta
     2521 atgcgctctt tttcttttaa gcgaaataat gtctttgtag aagtatattg aattccgagc
     2581 atatttattt cacgcagagc cgcagaggtt agattttaaa ttgaatttcc acactcgacc
     2641 cgtcattact gaaaatcacc acatcactca ggtgcttcat caggaagata ccgcgaccac
     2701 caacttcttc gatatgttcc ggactggtgg gatcggggag attgttgtag tcgaatccgc
     2761 agccctgatc ctgcacgcag cagcataggg tgttttgttc ggtgcgaatc tgcactttta
     2821 cctctttggt ggcatcgctc ttgttgccgt ggagaatagc gttgttaacg gcttcggtga
     2881 tgcagaccag catattgcca tacacctcat cagccaaatg caggcgctga cgcaactcct
     2941 ccacaaatgg ttcgatgagc gtaatgttaa gtggttccga tctgaggctg agctgtttcg
     3001 ataagggttg ttccgacatg ctaagcgcgg gtttattgat tgatgtcccg caaatattct
     3061 tctactagat ttttgtaaaa aggtttcaaa tccggtgaaa cagttttata aagatctaac
     3121 tcagcattgc gttttttgag gtattcctca atcgccggcg gtatagtacg gttgatttcg
     3181 ttggcggtat tgctctgtct ttcgttgtcc tgcttgcgct gacggtcgct ctcttctgct
     3241 tcaagcaggc gggtgagaat ttcttcctgg cgttttaacg tttcctgggt gatgcgcttg
     3301 ttcacaatgt cttcctcggt ctgatccatc ttatctgcta tctgctccaa ttgcttggca
     3361 agcttgccat cctctgtatt cccgccaccc aactgctcgg caagctcctg cagggcctga
     3421 cgcaatgccg cctgacgctg agccatttcc gccaattcct gactcatacc ctgattgcct
     3481 cccggtttct tcccctgctc catctgaccc ttcatcttgt tcagctgctc gttgagttgt
     3541 ttctgcatct cgctcatact cggcaattgc gagtcgctgc tgccaccggg tttctgacac
     3601 atctgactgc cgggcatgct cttggccatc tgttcctgca tctgttgcat cacctcatca
     3661 agcatcaggg cgaggttgtt gtaacccgtc atcacctgct gctggtacag attgctcttc
     3721 gccacctcgc gatcacccat ttgctccaca gtttccgcaa gtccgtcatt tacagccgat
//
LOCUS       DAOQOU010000241         3753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_16767_length_3753_cov_4.093294, whole genome shotgun sequence.
ACCESSION   DAOQOU010000241 DAOQOU010000000
VERSION     DAOQOU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16767_length_3753_cov_4.093294"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..2330)
                     /locus_tag="PLM90_13865"
     CDS             complement(141..2330)
                     /locus_tag="PLM90_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ90518.1"
                     /translation="MSPNRLQLVVISALSFSLCFATKASAQISCTFAVVDAADGNPLP
                     GAVIEIPAITYVNATDDNGYLHVTQLPSGKFSLHCSMISYQPLDTIIQLQHGAGDTVV
                     FSMYSALEHLEEVNISSTRTNARIEDDPTRIEVLGLEEMGEESTLVPTGIGSILGDLA
                     IITIQRTSVSTGNDEIRMAGMDPRYTQMLRDGLPLYSGASGGLGALDIPPLDLQQVEV
                     IKGSNSTLYGGGAIAGLINFVSRKPTKDLVIDGVLNMTSLLGSHASVFSGKQFEQLGY
                     TCFAAATINQPVNVKSDNYSIIPQEQQFIVHPRIFYTLHESTKLDVGFQWNHDKLNSG
                     FMDRAYHLQEGGYYAYNIADRFVEDITITHKWNAKSTLTFKSALSTFSRTQDVHTTDT
                     LATPFFSLQEMNGYTELSNAFHWGSHTFLAGYNFLFEQFDPGNEGLQLSKTAQYTQGI
                     FLQDDWHISEKFTCEIGLRGDVHSVYGSAVLPRIALFYKPAKDWSCRISGGSGYKTPN
                     ALDLSEPAKQLVTPVTSLHLERSEGVNADVNFHRLLNDHISLECNQALYYIMLQHPYA
                     LQTDDPENYVVVNDAGDVTSIGSDTYIRLEIDEIECYLGYNHTIAQKQQAINIYLPFY
                     PHDKLSGVLAYDNDTWRMGIEASYQANQYLSDQTKAPDYLFMAAMISRSLGHFRLTLN
                     GENLTNAIQQNLTDIPIVSGSLLEPTFSDIWAPIEGITINASLKYTF"
     gene            complement(2400..3477)
                     /gene="prfB"
                     /locus_tag="PLM90_13870"
     CDS             complement(join(2400..3416,3418..3477))
                     /gene="prfB"
                     /locus_tag="PLM90_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217450.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HMZ90519.1"
                     /translation="MTQEHYNELMDKLLVLRRFLNYDDKLIQVKEDEQITHQPGFWDD
                     PKNAEVVLKRIKINNVWINSYNEAQQAVDDLGVLYEFSQMGEATEEEVDAQFAKTKER
                     LEELEFTSTLTEKEDALPAILTINAGAGGTESCDWASILYRMYMMWANKKGFTVNELD
                     FQAGDVAGIKSASLEIDGEFAYGLLKGENGVHRLVRISPFDANAKRHTSFASVYVSPM
                     IDDTIEINISPSDLTWDTFRSGGAGGQNVNKVETAVRVTHLPTGIVVSCQVNRTQGQN
                     RELAMQMLRSKLYDLEMQKRQEVKDKIEGTKKKIEWGSQIRNYVLHPYKLVKDARTGH
                     ETGNAQAVLDGDLDDFIKAYLLAE"
BASE COUNT          987 a          838 c          825 g         1103 t
ORIGIN      
        1 ccaacagcac cgagcggaaa tgaccccttg acataaagca tgaaaaatgg catatccgag
       61 tgtggagctg gtttgaaata tttgtatttc gcaagcagat attcaccgca aaaaaattca
      121 ccttgatccg cagagattga tcagaatgta tatttcaatg aggcatttat cgtgatgcct
      181 tcaattggtg cccaaatatc agagaaagta ggttctaaaa gcgaaccgct aacgatagga
      241 atatccgtta aattttgctg gatggcattg gtgagatttt caccattcaa agtcaaacgg
      301 aaatgcccga gcgatcttga gatcattgca gccataaata gataatcagg tgccttggtt
      361 tgatctgaaa gatattgatt cgcttgatag ctggcttcta tacccatgcg ccaagtatca
      421 ttatcgtagg ctaatacgcc ggataattta tcatggggat aaaagggtaa atagatattt
      481 atcgcttgtt gtttttgcgc gatggtgtga ttatatccaa gataacattc gatttcatcg
      541 atctccaatc tgatatatgt gtcagatccg atacttgtga catcaccggc atcattcacc
      601 accacataat tttcagggtc atctgtctgt agtgcatatg gatgctgtaa catgatataa
      661 tataatgctt gattgcattc tagagatata tggtcattga gcaggcgatg gaaatttaca
      721 tcagcattca caccttcaga acgctctaaa tgaagggatg taaccggtgt aacaagttgt
      781 ttggccggtt cagaaagatc gagcgcattt ggtgttttgt atccactgcc accactgatc
      841 ctgcaactcc aatctttagc aggcttataa aacagggcga tgcggggaag aacagctgac
      901 ccatacacac tatgcacgtc gcccctcagg ccgatctcgc atgtgaattt ttcacttata
      961 tgccagtcgt cttgtaagaa aatgccttgt gtgtattgtg ctgtctttga taattgcaat
     1021 ccttcattcc ctgggtcgaa ctgttcgaac aggaaattgt atccagcaag gaatgtatgt
     1081 gatccccaat gaaaagcatt tgataattct gtgtagccat tcatttcctg tagactgaaa
     1141 aatggggttg ccagtgtatc tgtagtatgc acatcctgtg ttctggaaaa tgtgcttagc
     1201 gctgatttga atgtcaaggt gctttttgca ttccacttgt gtgtgatcgt gatatcttcc
     1261 acgaaacggt cggcaatatt atatgcataa tagccacctt cctgcagatg gtaggctctg
     1321 tccatgaaac cagaattgag tttatcgtga ttccattgaa atccaacatc cagtttagtt
     1381 gattcatgca atgtataaaa aatacgtgga tgcacgatga actgttgttc ttgtgggatg
     1441 atggaatagt tgtcagactt cacatttacc ggctgattga tagtagctgc tgcaaagcag
     1501 gtgtatccta attgttcgaa ttgtttacct gaaaaaacac tggcatgcga ccccagaaga
     1561 gatgtcatat ttaatacacc atctatcacc agatcttttg ttggttttct ggatacaaaa
     1621 tttatcaaac cagcaattgc tccgccgcca tacaatgttg aattgcttcc ttttatcact
     1681 tccacctgct gaagatcgag tggggggata tcgagtgcgc cgagtccacc ggaggctcct
     1741 gaatacagtg gaagcccatc gcgcagcatc tgggtgtatc gtggatccat ccctgccatg
     1801 cgaatttcat cattacctgt ggaaacggaa gttcgctgta tggtaataat ggccagatct
     1861 cccaaaatgc tacctatacc tgttggcacc agcgtgctct cttcacccat ttcttcgagt
     1921 ccgagtactt caatacgagt aggatcatct tcgatgcgtg catttgttct cgtgctgctg
     1981 atgtttactt cttccagatg ttcaagagcg ctatacatcg aaaaaacgac tgtatcaccg
     2041 gctccatgct gaagttgtat tatcgtatca agaggttgat aagatatcat tgagcaatgc
     2101 agactgaact ttcccgaagg cagttgggta acatgcaaat aaccattatc gtctgtcgca
     2161 ttgacataag taatggcagg gatttcaata acagcaccgg gcagcggatt tccgtcggcc
     2221 gcatctacta ctgcaaaggt gcagctgatc tgtgcggatg ctttcgtggc gaaacacaaa
     2281 ctaaaactca gtgctgaaat tacaaccagc tgtaagcgat ttggcgacaa tgcaagtaat
     2341 tatgcaataa aggtaacacg aagatgtaca tgtcactatt aaagcttgtt aaactgaaat
     2401 tattctgcca gcaggtaagc cttgataaaa tcatccaggt cgccatccag aacggcttgt
     2461 gcattgcctg tctcgtgacc ggtgcgggca tctttcacga gtttatacgg gtgcaataca
     2521 tagtttcgga tctggcttcc ccattcgatc tttttcttgg tgccttcgat tttatctttc
     2581 acctcctgac gcttttgcat ttcgagatca tacaacttac tgcgcaacat ctgcatggcg
     2641 agttcgcggt tttgtccttg cgtacgatta acctggcaag aaactacaat ccctgttgga
     2701 aggtgtgtaa cgcgaactgc agtttccacc ttgtttacgt tttgtccacc tgcaccacca
     2761 cttcggaagg tatcccatgt aaggtcgctc ggagaaatat taatctctat cgtgtcatcg
     2821 atcattggcg aaacatacac ggaagcaaat gaagtatgtc gcttcgcatt ggcatcgaat
     2881 ggtgagatgc gcactaaacg atgaacacca ttttccccct tcagcaatcc gtaggcaaat
     2941 tcaccatcta tttctaaaga cgctgattta attcctgcaa catcaccggc ctgaaaatcc
     3001 aattcgttca ctgtgaagcc ttttttgttg gcccacatca tgtacatgcg atatagtata
     3061 ctcgcccaat cgcagctttc ggttccaccg gcacctgcgt taatggtgag aatagcgggt
     3121 aaggcatctt ctttttcggt gagggtggat gtaaactcta attcctccaa ccgctctttc
     3181 gtttttgcaa actgagcgtc tacttcttct tcggtagcct cgcccatttg cgagaattca
     3241 tacagcacac caaggtcatc aactgcttgt tgcgcctcgt tataactgtt gatccataca
     3301 ttgttgatct tgattcgctt gaggaccact tccgcatttt tcgggtcatc ccagaaaccc
     3361 ggttggtgag tgatctgttc gtcttctttt acctggatga gcttatcatc gtagttaaag
     3421 aaacctcctc aaaacaagca atttatccat tagctcgtta taatgttctt gtgtcatggg
     3481 gcaaagatag gaaaaaggag agaggagaga ggagagagga gagaggagag gggtgagtgg
     3541 gtgagtgggt cagtgggtca gtgggtgagt taattgcgcc ttagcttctt cgctgtcttt
     3601 gcgcgaaatc caccccacct gctaacttgc taacccttag cttcttcgct gtttttgcgc
     3661 gaaacccacc ccacctgcta acttgctaac cctcaaactg ggtgagttaa ttgcgcctta
     3721 gcttcttcgc tgtctttgcg cgaaatccac ccc
//
LOCUS       DAOQOU010000242         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_17715_length_3641_cov_4.336308, whole genome shotgun sequence.
ACCESSION   DAOQOU010000242 DAOQOU010000000
VERSION     DAOQOU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17715_length_3641_cov_4.336308"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1065
                     /gene="ftsY"
                     /locus_tag="PLM90_13875"
     CDS             115..1065
                     /gene="ftsY"
                     /locus_tag="PLM90_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMZ90520.1"
                     /translation="MGLFDIFKREQKEDLNKGLEKTKQSLFSKITKAIAGKSRVDDEF
                     LDELEQILISSDVGLDTTIKIIDRLNKRVSRDKYMGADELNAIMKDEIVQMLSENNTV
                     DYTDVVIPQNVKPYIMLIVGVNGVGKTTTIGKLAWQFKQQGKKVLLGAADTFRAAAVD
                     QLTIWSERAEVPIVKHQMGADPAAVAFDTVNAAKSQDADVVLIDTAGRLHNKVNLMNE
                     LSKIKRVIQKVYPDAPHDVMLVLDASTGQNALEQAKQFTAATDVTCIALTKLDGTAKG
                     GVVLGIADQFKIPVRYIGVGEKMHQLQVFNKKAFVDALFE"
     gene            1084..2394
                     /gene="rimO"
                     /locus_tag="PLM90_13880"
     CDS             1084..2394
                     /gene="rimO"
                     /locus_tag="PLM90_13880"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HMZ90521.1"
                     /translation="MKARTLKKDKVNIITLGCSKNTVDSEVLLSQLKANDVQAFHERE
                     KDDCNIIIVNTCGFIDKAKQESIDTILNYAEAKSNGEIEKLYVTGCLSHRYKDELQQE
                     IPEVDAWFGTMELPAILKRFNADYKHELLGERMLSTPAHYAYLKISEGCNRTCSFCAI
                     PLMRGQHVSRTIEDLVKEAQYLASVGVKEIMLIAQELTYYGLDIYKKRALSDLLKELD
                     KVEGIEWIRLHYAYPSKFPLDVIETIRDSKHICRYLDMPLQHASDNMLKAMNRQITLE
                     ETKELITNIRNIIPNITLRTTMLVGFPGETEEDIDALCAFVEWAKFDRLGVFTYSHEE
                     GTSGYALNDDIPAEVKAERAARVMEVQEEISIAKNAAKVGSTQRVLIDRIEGGFFIGR
                     TAADSPEVDNEVLIDASAHYLRVGDFANIHISRAESFDLYGEPV"
     gene            2416..>3641
                     /gene="bshC"
                     /locus_tag="PLM90_13885"
     CDS             2416..>3641
                     /gene="bshC"
                     /locus_tag="PLM90_13885"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HMZ90522.1"
                     /translation="MQHQVIPFDTTKRFSKIVTDYISGADALKPFYAWSPEFASFPAI
                     IEGKSKESIDRKALVTMLRKQYAGINIIDAVANNINAFAKENTFCVVTAHQVNLFTGP
                     LYYLIKTASTIQLCNTLNKQFPNFRFVPVFWQGSEDHDFAEINHAHIFNKTFTWETDQ
                     RGATGRFSLKDAQPLVQELLSMLGDGPYAKEMHHILSDAYLNGGTLADAGRKMLNALF
                     GKYGLVVVNGDDHYAKTVFANIMEDELLNSSSVSLVNKTITQFPYEAQAHPRDINLFY
                     LDNHGRNRIIADEQHQMFMVKDTDVTMTREEITYQLRKFPERFSPNVILRPVFQQKLL
                     PSIAFIGGGGEIAYWMQLKSVFEYHQVQYPMLLLRDSFMIIDHATNKKIQKLQLNIAD
                     LFEDVSGLTGEYVRRN"
BASE COUNT         1147 a          729 c          776 g          989 t
ORIGIN      
        1 aactaatggt aaaagcttct atttccatag aggctttttt tgtttatagc aaacagacct
       61 tttggaacaa accatcttcc gaaaacatta catcatcaca gcagtaacaa acacatgggt
      121 ctcttcgata tattcaaacg cgaacaaaaa gaggatctca acaaagggct cgaaaaaaca
      181 aaacagagcc tcttctccaa gatcaccaaa gccatcgccg gaaaatcgcg cgtagatgac
      241 gaatttttgg atgaactgga acagattctc atatccagtg atgttggcct cgataccacc
      301 atcaagatca tcgaccgcct caataaacgt gtgtcgcgcg ataaatatat gggcgccgat
      361 gaattgaatg cgatcatgaa agatgagatc gttcaaatgt tgtcggaaaa caataccgtt
      421 gattataccg atgtcgttat ccctcaaaat gtgaaacctt atatcatgct catagttggt
      481 gtgaatggtg ttggcaaaac cactaccatc ggaaaacttg catggcaatt taaacaacaa
      541 ggtaaaaaag tattactcgg tgccgccgat acctttcgcg cagctgccgt agatcaatta
      601 actatctggt cggaacgtgc tgaagtgcct atcgtaaaac atcagatggg cgccgatccc
      661 gcagcagtgg catttgatac cgtaaacgca gctaaatcgc aagacgctga tgtggtgctg
      721 atagatactg caggaagatt gcataataag gtgaacctga tgaatgaact ttctaagatc
      781 aagcgcgtaa tacagaaagt atatccggat gcaccgcacg atgtcatgct cgtactcgat
      841 gcatctaccg gtcaaaatgc gctcgaacaa gcaaaacaat ttactgcggc taccgatgta
      901 acctgtatcg ccctcaccaa actcgatggc accgctaaag gtggcgttgt attaggtata
      961 gctgatcaat ttaaaattcc tgttcgctat atcggtgttg gcgaaaaaat gcatcaactg
     1021 caggtgttca ataaaaaagc atttgttgat gccttatttg aatgatagca caatgattat
     1081 aaggtgaagg caagaacact caaaaaagat aaggtcaata tcattacact cggttgtagt
     1141 aaaaataccg ttgatagtga agtgctgttg tcgcaactga aagccaacga cgtgcaggca
     1201 tttcacgaac gtgagaaaga tgattgtaat atcatcatcg tgaatacctg cggatttatt
     1261 gataaagcaa aacaagaatc tatcgacacc atcttgaatt atgccgaagc aaaatctaat
     1321 ggtgaaattg aaaaattgta tgtaaccggt tgtctcagtc atcgatataa agatgagctt
     1381 caacaagaaa tccctgaggt ggatgcatgg tttggtacca tggaacttcc tgccatcctc
     1441 aaacgcttta atgccgatta taaacacgaa ttgctgggcg aacgcatgtt gtcgacacct
     1501 gcacattatg cctatctgaa aatatctgaa ggttgtaatc gcacttgttc attttgcgcg
     1561 ataccattga tgcgcggaca acacgtttcc cgtacaatag aagatcttgt caaagaagcc
     1621 caatatctcg caagcgttgg ggtgaaggag atcatgctca ttgctcagga actcacgtat
     1681 tacggactcg atatttataa aaaacgtgcg ctttctgatt tgttgaaaga attggataag
     1741 gtggaaggaa ttgaatggat acgcctgcat tatgcttatc catcaaaatt tccattggat
     1801 gtgattgaaa ccattcgcga cagcaagcat atttgtcgct acctcgacat gcctttgcaa
     1861 catgcttcag ataatatgtt gaaggcaatg aatcgtcaga ttacattaga agagactaaa
     1921 gaattaatta ccaatatcag aaatattatt cccaatatta ctttgcgtac aacaatgttg
     1981 gttggattcc cgggagaaac ggaagaggat attgatgcgt tgtgtgcatt tgtggaatgg
     2041 gcgaaattcg atcgccttgg ggtgtttact tattcgcatg aagaaggaac aagcggctat
     2101 gcattgaatg atgatattcc cgctgaagta aaagccgaac gcgcagcaag agtaatggaa
     2161 gtgcaggaag aaatctctat cgccaaaaat gctgctaaag tgggtagcac acagcgcgtg
     2221 ctcatcgacc gcattgaagg aggattcttc attggcagaa cagcagccga ttcacctgaa
     2281 gtggataatg aagtattaat tgatgcatct gcgcattatc ttcgtgtagg cgattttgcc
     2341 aacattcata tctcccgtgc cgaatcattt gatctgtatg gggaacccgt atgagcaaac
     2401 tcgcgttgac accgcatgca gcatcaagta ataccttttg ataccacaaa gcgattttct
     2461 aagatcgtta ccgattatat ttccggagcg gatgcattaa aaccttttta tgcctggtcg
     2521 cccgaatttg catcgtttcc cgcaatcatt gaagggaaaa gcaaagaatc aatcgataga
     2581 aaagcactgg taactatgct gcgcaagcaa tatgccggta ttaatattat tgatgccgtt
     2641 gcgaataata tcaacgcatt cgcaaaagaa aatacctttt gtgtggttac ggctcatcag
     2701 gtgaatttat ttacaggtcc attgtattat ttgattaaaa cagcatcaac aatacaactc
     2761 tgtaatactc taaacaaaca atttcccaat ttccggtttg ttcctgtttt ttggcaggga
     2821 agcgaagatc acgatttcgc tgagatcaat catgcccata tttttaataa gaccttcaca
     2881 tgggagactg atcaacgtgg cgcaacaggt agattttctt tgaaagatgc acaaccgctc
     2941 gtgcaggaat tattgagcat gcttggcgat ggtccttatg ccaaggaaat gcaccatatt
     3001 ttatcagatg cttatctaaa cggcggaaca cttgcagatg cgggcagaaa aatgttgaat
     3061 gcattgtttg gtaagtacgg gcttgttgtt gtgaatggcg acgatcatta tgcaaaaacc
     3121 gtattcgcca acatcatgga agatgagctc ttgaacagca gttcagtttc actcgtcaat
     3181 aaaacgatta cacagttccc gtacgaagca caggcacatc cacgagatat taatttattc
     3241 taccttgata atcatggtcg caatcgcatc attgctgatg aacaacatca gatgtttatg
     3301 gtgaaggata cggatgttac catgacacgc gaagaaatca cctatcagtt gcgaaaattt
     3361 cctgagcgat ttagtccgaa cgtaatatta agacctgtat tccaacaaaa attattgccc
     3421 tccattgcat ttataggtgg aggaggtgaa attgcttatt ggatgcaatt gaaatcggta
     3481 ttcgaatatc atcaagtaca atatccaatg ttgttgctgc gcgattcttt tatgatcata
     3541 gatcatgcta ccaataaaaa gattcaaaaa ctgcaattga acattgccga tttatttgaa
     3601 gatgtatccg gtttaaccgg cgaatatgtt cgtcgcaata g
//
LOCUS       DAOQOU010000243         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_18081_length_3600_cov_5.837518, whole genome shotgun sequence.
ACCESSION   DAOQOU010000243 DAOQOU010000000
VERSION     DAOQOU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18081_length_3600_cov_5.837518"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLM90_13890"
     CDS             complement(<1..658)
                     /locus_tag="PLM90_13890"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ90523.1"
                     /translation="MRKFLILYVSLLLAIPVFAQDDEDLPARPVPAHLVNDLGNVFAA
                     DQEMMLEQKLLAYNDSTSTQITVVTIASVQPYEIGDYAYKLGRKWGVGQADKDNGIVI
                     LVAVSDHKVFIATGYGIEGYLPDIATSHIINDNFLPAFRKNDYYGGINAGLDEMYKYL
                     TGAYEGNPYGSGSPSDIPWGTVIIIVIVILFIISSRNKRNGGTTFTGGGRRTWGYGGG
                     Y"
     gene            complement(651..1094)
                     /locus_tag="PLM90_13895"
     CDS             complement(651..1094)
                     /locus_tag="PLM90_13895"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ90524.1"
                     /translation="MPKARDFFTAEQQQQIVHAIKQAELKTSGEIRVHLEEKSGGDAY
                     GRAVKLFSSLKMDKIELHNGVLIYLAVVDRQFAIIGDSGIHAKVRQEFWDSMKEAMVS
                     HFRNEDFTGGLIAAITSAGEQLQQYFPRSSDDKNEMTDEISFKDA"
     gene            complement(1100..1726)
                     /locus_tag="PLM90_13900"
     CDS             complement(1100..1726)
                     /locus_tag="PLM90_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMZ90525.1"
                     /translation="MTTTTPAAAARNPKMGRYIGIGIVVVLILYLISLYNGIMNADET
                     VNQKWGDVESNYKRRYDLIDNLVNTVKGAADFEKSTLTELVEARAKATSITIDPATAT
                     PEQIKAFMAAQSGVSTSLGRLLAVVENYPQLTATQAYRDLMVSLEGTENRINVARMDY
                     NEAVKNYNVRIRKFPGNLIAGIAGLDRRNTFDAPEGVEDVPKVDFGTK"
     gene            1857..2201
                     /locus_tag="PLM90_13905"
     CDS             1857..2201
                     /locus_tag="PLM90_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ90526.1"
                     /translation="MSDQVTAFNEYRSKMNEVILSKQNKVINRLFNLDTNTYAEGALD
                     VKTKEMLGLVASMVLRCDDCIKYHLGKCHEQGITTDQMYEIFAVANIVGGTIVIPHTR
                     RAAEYWEELLKV"
     gene            2580..>3600
                     /locus_tag="PLM90_13910"
     CDS             2580..>3600
                     /locus_tag="PLM90_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HMZ90527.1"
                     /translation="MANEEKKLYLIDAFALIFRAYFAFARNPLINSKGQNVSAISGFV
                     NTLYDLLNNTNSTHIAVCFDSPEPSQREEMYADYKANREEAPEDIKFAVPYIKEIVKA
                     FHIPIIEKGGYEADDIIGTLAKKAKQAGYEVYMVTMDKDYGQLMEEGIFMYRPSYTGK
                     GFDTLTVDDILQKWEIERPEQVIDILGLMGDAVDNIPGVPGVGEKTAKKLIQEFGTVE
                     GLYENTDKLKGKLQENVVNNKEQALMSKVLATIMLDVPIEFEEDKLIIEEPDKEKLAA
                     IFQELEFRQLGKRIIGDSYSANVEKKTEQLDLFGNPTTTTSSQNAIRESREAVTAGKD
                     ISNVQH"
BASE COUNT         1074 a          827 c          725 g          974 t
ORIGIN      
        1 aatatccgcc accataaccc catgttctgc gaccgccacc ggtgaatgta gttccgccat
       61 ttcttttatt gcgactggaa ataataaaaa gaattacgat gacaataatg attactgttc
      121 cccagggaat atcagatggc gatccactgc catacggatt cccttcatag gcacctgtca
      181 ggtatttata catttcatca agcccggcat ttattccgcc gtaataatcg ttcttacgaa
      241 atgcaggtaa aaaattatcg ttgatgatat gtgatgttgc aatatcaggt aaataacctt
      301 cgataccata acctgttgca atgaatactt tatgatcgct aaccgcaact agaattacaa
      361 taccattatc tttatcagcc tgcccaacgc cccattttct tcctaattta taagcataat
      421 caccaatttc atatggttgc acagaagcta ttgtgacaac ggtaatctgc gttgaagtag
      481 aatcgttata tgccaataat ttttgctcca gcatcatctc ctgatctgca gcgaaaacat
      541 tgccgaggtc attcacaaga tgcgccggaa ccggacgcgc gggcagatcc tcatcgtcct
      601 gcgcaaacac aggaattgca agcaacaggc ttacatataa gatcagaaat ttacgcatcc
      661 ttgaaagata tctcatcggt catttcgttc ttatcgtccg atgatcgtgg aaaatattgt
      721 tgtaattgtt ctcctgctga agtgatagca gcaatcaaac cacctgtaaa atcttcattg
      781 cgaaaatgcg acaccatcgc ctccttcatg ctatcccaaa attcctgacg cacttttgca
      841 tgtatacccg aatctccgat gatggcaaat tgtctatcca ccacagcaag ataaatcagt
      901 acgccattgt ggagttctat tttatccatt ttcagcgaag aaaacaattt cacagcgcga
      961 ccataagcat ccccacccga tttttcttcc agatgcacac gtatttcacc tgaggttttt
     1021 aactcagcct gcttgatagc atgcacaatc tgctgctgtt gttcagcagt gaaaaaatca
     1081 cgcgcttttg gcatgtcgat tattttgtac cgaagtcaac cttaggcaca tcttcaacac
     1141 cttccggtgc atcaaatgta ttgcgacgat cgagacctgc aataccggca attaggttac
     1201 cggggaattt acgaatacgc acattgtaat ttttcaccgc ttcgttgtaa tccatgcggg
     1261 ccacattgat acggttttct gtgccttcca atgacaccat cagatcgcga tatgcctgag
     1321 tagcggtaag ttgaggataa ttttctacta cagcgagtaa gcgacccaag gaagtagata
     1381 cgccactttg tgcagccata aatgctttga tctgttcagg tgttgccgtt gcaggatcta
     1441 tggttatgct tgtagctttt gctctcgcct caaccaactc ggtaagggtt gatttttcaa
     1501 aatctgccgc accttttacg gtgtttacca ggttatcgat gaggtcataa cggcgtttgt
     1561 agttagattc cacatcaccc catttttggt taacagtttc atctgcgttc atgatgccgt
     1621 tgtagaggct tatcagataa aggatgagca ctaccactat accaataccg atatagcgac
     1681 ccattttcgg gttacgagca gcagcagcag gtgttgttgt tgtcattttc aggttgtttt
     1741 gtcaggtaaa gatagggtat accacccaat cagaccataa agacggaatc acgaccaaaa
     1801 tattgtaaag tttggagttt cctgccccgt gactgaactt tggccaaaac cctgctatga
     1861 gcgatcaagt aactgctttc aacgaatacc gttcaaaaat gaacgaggtg atcctttcca
     1921 agcaaaataa agtgatcaac cgactcttca acctcgatac caatacctat gcagaaggcg
     1981 ccctggatgt caaaaccaaa gaaatgctcg gccttgtagc cagtatggtg ctgcgttgcg
     2041 acgactgtat caaataccac ctcggcaaat gccacgaaca aggtatcacc acagatcaga
     2101 tgtatgagat attcgctgtt gccaatattg taggcggaac aatcgtgata ccgcacacaa
     2161 ggagagcagc ggagtattgg gaagaactgc tgaaagtgtg aaagtcccgc tgagggcggg
     2221 atgtcgctgc gctccatgtg aaggtgtgaa agtgtttagg tttgtggctc tgttcatact
     2281 ctccttcgct aactcccgca aactctgtga tttaacttct gagaatcaaa cggactatca
     2341 ctacttcgcg tctttgcccc ttagcacctt cgcgcgaaca caacagtttg cccattccca
     2401 catggagcgc agcgacatcc cagccaaagc tggacccgca cacctcaatt gcatttcact
     2461 tgcacatttg cagacttgca cacttgcaca ctctcgaaag acaccacctc ctgacattcc
     2521 actagttaat tggctaattc tccacctttc cctatcttgc aaacccttat tttaatatca
     2581 tggctaacga agaaaaaaaa ttatatctca ttgacgcatt tgcgttgata ttcagggctt
     2641 attttgcgtt tgcgcgcaat ccgctgatca attcgaaagg ccagaatgtt tctgcaatct
     2701 caggatttgt aaatacctta tacgatctgc tgaataatac caattcaaca catattgcag
     2761 tatgcttcga ttcaccggag ccttctcaac gcgaagaaat gtatgccgat tacaaggcaa
     2821 accgcgaaga agccccggag gatatcaaat tcgctgtgcc atatatcaag gaaattgtaa
     2881 aagcatttca tattcccatc atagaaaaag gaggatatga agctgatgat atcatcggca
     2941 cgctcgcaaa aaaagcaaaa caagccggat acgaagtata tatggtgacc atggataaag
     3001 attatggtca attaatggaa gaaggcattt ttatgtatcg tcccagttat accggaaaag
     3061 gatttgacac cctcaccgtt gatgatattt tgcagaaatg ggaaattgaa cgcccggaac
     3121 aggtaataga tatcctcgga ctgatgggcg atgcagtgga taatattccc ggcgttcccg
     3181 gtgttggaga aaagacggcg aaaaaattaa ttcaggaatt cggaacagtt gaaggattat
     3241 atgaaaatac cgacaaattg aaaggcaaac tgcaggaaaa tgtagtaaat aataaagagc
     3301 aagcattaat gagtaaagta ttagctacca tcatgctcga tgtgccaatc gaatttgaag
     3361 aagataagct cattattgaa gaaccggata aagaaaaact tgcagctatt tttcaagaat
     3421 tagaattccg tcagctcggc aaacgcatta ttggcgacag ttactcagcc aacgttgaaa
     3481 agaaaactga gcaacttgat ctctttggaa accctactac tacaacatct tctcaaaatg
     3541 ccattcgcga aagcagagaa gctgttacgg ccggaaaaga tatttcgaat gttcaacata
//
LOCUS       DAOQOU010000244         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_18286_length_3575_cov_5.010227, whole genome shotgun sequence.
ACCESSION   DAOQOU010000244 DAOQOU010000000
VERSION     DAOQOU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18286_length_3575_cov_5.010227"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..728
                     /locus_tag="PLM90_13915"
     CDS             216..728
                     /locus_tag="PLM90_13915"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ90528.1"
                     /translation="MREFIIALSLPVSGIISFPQSGCGQTICTPKEIRTEIIINAPSA
                     EAWKLLTDFDAYSGWHPYILKIEGKIALKEKIKVTTIDADSTADQFSAFVLELEPEKK
                     LSWGGSLGFIFSAKHYFIIEPINDDQIRFVQGEYWHGLFGKHYGEKIYLDTFQKFIAM
                     NEKMKILLEG"
     gene            1247..1681
                     /locus_tag="PLM90_13920"
     CDS             1247..1681
                     /locus_tag="PLM90_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90529.1"
                     /translation="MKKNWKIILLVVVILGGIGFWYGMQQYNRKPKGAGDMKAEFTMT
                     AADLTKAFTDDEAGSGTKYNAKPMLISGTVRGVEKDDAGIYTITLAGDGDMSNVVCTM
                     SNEESGLDTVKEGDALNIQGFCNGAQTMLGTDVLMNRCAIKK"
     gene            1698..2261
                     /locus_tag="PLM90_13925"
     CDS             1698..2261
                     /locus_tag="PLM90_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ90530.1"
                     /translation="MMMRKVVMVFAVQLMFLSFVQAQGKIYTKTGTINFDATADMEKI
                     AAVNKKVTAVIDTKSGAMEFAVLMKSFEFEKALMEEHFNENYVESDTYPKATFKGTIS
                     NLSDIKFTTDGTYTANVTGNLTMHGVTKPVTTTGTFTVKNGKVTAKSTFKVLLSDYNI
                     TIPSLVAANISKEVTITVDLALEPLPQ"
     gene            2326..3204
                     /locus_tag="PLM90_13930"
     CDS             2326..3204
                     /locus_tag="PLM90_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HMZ90531.1"
                     /translation="MQKLLIVLLLLVGLNATAKAQDDLLSMLGDDSTTTEYVKNAFKS
                     SRVINGQSMEMIPKGALDFRILHRFGEVSGGAYEFFGLDQASMRMGFDYGILKNLSVG
                     VGRSTAQKELDGFVKYRILQQSTGAKVMPFSLVYVGGVTCNGMKWADTSVTNYFTSRL
                     AFYNQLVIGRKFNESITLQLSPTFLHRNLVIYDADPSDIYSLGVGGRVKVTRRIALTG
                     DYFYVFNNNDSTAVNPLSIGVDIETGGHVFQLHFSNCVGMNERAFLLDTKGQWSKAEV
                     RFGFNLSRIFQLADSH"
     gene            complement(3264..>3575)
                     /locus_tag="PLM90_13935"
     CDS             complement(3264..>3575)
                     /locus_tag="PLM90_13935"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMZ90532.1"
                     /translation="KLEADPTVKFAIGDFTIKRILFADLEIDSPYNTYKYPGLPPGPI
                     HMPPIAYIDAVLHYEHHDYIFMCARGDGSGYHLFAKTLAEHNRNRDIYIRTLNKAGIK
                     R"
BASE COUNT         1113 a          745 c          739 g          978 t
ORIGIN      
        1 cggtagacac gcaggactta aaatcctgtg ggcagtaatg tccgtgcggg ttcaagtccc
       61 gccccgggta cttaaattaa ttctcgcgta gaattatctt caatgtaacc tttttgcggg
      121 cctgcctgcc gtatgatagg ttcaagtcac gccccttaag ctccccaata tttagctaca
      181 tttgaaatca gcaaaactcc tgacttcaaa atattatgcg cgaatttatt attgcactta
      241 gtttacctgt atcaggcatt atatcatttc cacaatccgg ttgcggacaa acaatatgca
      301 ctcccaaaga aatccggaca gaaataatca tcaacgctcc ttcagctgaa gcatggaaat
      361 tacttaccga ttttgatgcc tattcaggct ggcatcctta tattttaaaa attgagggta
      421 aaatcgcttt gaaggaaaaa attaaagtaa ccaccatcga tgcggattct actgcagatc
      481 aattctcggc atttgtttta gagttagagc cggaaaaaaa actatcctgg ggtggatcat
      541 tgggttttat ttttagtgcc aaacattatt ttataattga acctataaat gacgatcaaa
      601 tacgttttgt gcaaggtgaa tattggcatg ggttattcgg gaaacattat ggcgaaaaaa
      661 tatacctgga tacatttcaa aagtttatcg ccatgaatga aaaaatgaaa atacttttgg
      721 aaggataatt gtaattcaaa acgattcaat agaagatagc ttcgacaggc aaagtgatga
      781 agaactatta tccttcagga tcaccaaaca atcagtaacc cttttttcgc gcgaagacgc
      841 aaagatgcaa aggcgcaatg caactatcgc ccctttctca cgaccaatct gctcggaggt
      901 taaactcacc tacccgcctg ttcggcgagg taaactcaac cacccgcctg ttcggcgagg
      961 taaactcacc cacccgcctg ttcggcgagg taaactcacc cactcacccc ccaacccctt
     1021 ctaaatcaac aatttacctc cgtctcagaa ggaaccaaaa tgctcatttt tacgtttaca
     1081 aggcataaaa ggaacccaaa tcccttaaaa aacacggaaa tcagctaaaa gtgtctaagg
     1141 atggcttgca cagagggttg taactttgac ccgccaatca atgcaaggta cctggcatca
     1201 ataggcacga tacttgtaat aacatttaac acataaaaac ccaactatga aaaagaactg
     1261 gaaaattata ctgcttgttg ttgtaatcct tggcgggatt ggcttttggt acggcatgca
     1321 gcaatacaac cgcaaaccta agggcgcagg cgatatgaag gcagaattca ccatgactgc
     1381 tgccgatctt acaaaggcat ttactgatga tgaagcgggc tcaggaacaa aatataatgc
     1441 taagcccatg ctcataagcg gaaccgttag gggtgttgaa aaagatgatg caggaattta
     1501 taccatcacc cttgccggcg atggcgacat gagcaatgtt gtatgcacca tgtcgaatga
     1561 agaaagtggt ctagataccg ttaaagaagg tgatgcttta aatatccagg gtttctgcaa
     1621 tggtgctcaa accatgcttg gaactgatgt attaatgaat agatgtgcta ttaaaaaata
     1681 acaataaaac aatgaatatg atgatgagaa aggtagtaat ggttttcgct gtgcaattga
     1741 tgttcctgag ttttgttcag gcgcaaggca agatctacac gaaaacaggc accataaatt
     1801 ttgatgccac tgccgacatg gaaaagatcg ctgcagttaa caaaaaggtt acggctgtta
     1861 tcgacaccaa aagcggtgca atggaatttg cagtgttgat gaaatcgttt gaatttgaaa
     1921 aagcactgat ggaagagcac ttcaacgaga attatgttga aagtgatact tatccaaaag
     1981 caacattcaa aggaaccatc tcgaatttaa gtgatatcaa attcactaca gatggcactt
     2041 ataccgcaaa tgttacagga aaccttacca tgcatggtgt tacaaagcct gttaccacta
     2101 caggcacctt caccgtgaaa aacggcaagg tgacagcaaa atcgacattc aaggtcttgc
     2161 tatccgacta taatatcaca attccttcgc tggtggcagc aaatatcagt aaagaggtaa
     2221 ccattaccgt cgacctcgca ctggaaccac tcccacagta aaacttttaa attcctgaac
     2281 atatctttgt tcaggaattt tttttgtttc ctcaaatacc aaccaatgca aaaattatta
     2341 attgttctgc tccttctcgt aggactgaac gcaactgcaa aagctcagga tgacctattg
     2401 agcatgctgg gcgatgactc aacaacaact gagtatgtaa aaaatgcctt taaatcttcg
     2461 cgcgtaatca atggacagtc gatggaaatg atcccgaaag gggctctcga tttccgtatc
     2521 cttcaccgtt ttggcgaagt gagcggtgga gcttatgaat tctttggcct tgatcaagcg
     2581 agcatgcgta tgggttttga ctatggcatc ctaaaaaatc tatcagtagg tgtcggcaga
     2641 tctacagctc aaaaagagct cgatggattt gtaaaatatc gtatcctaca acaatctacc
     2701 ggagctaagg tgatgccctt ttcattggtg tacgttggcg gcgtgacctg caacggaatg
     2761 aaatgggcag atacatctgt gaccaattat tttacttcgc gacttgcatt ttacaaccaa
     2821 cttgtgattg gaagaaaatt caatgagagc attaccctcc agctttcacc aacatttttg
     2881 catcgcaacc tcgtaatcta tgatgcagac ccaagtgata tttattcatt aggcgtaggt
     2941 ggtcgcgtga aggttacacg tcgtattgca ctcaccggcg attattttta tgtgttcaat
     3001 aataacgata gcactgccgt aaatcctttg agcattggtg ttgatattga aaccggtggt
     3061 cacgtattcc agttgcattt cagcaattgt gtaggtatga atgaacgcgc atttcttctg
     3121 gatacaaagg gacaatggtc gaaagctgaa gtacgcttcg gatttaattt atctcgtatt
     3181 ttccaactgg ccgacagcca ttaataaaat aatattcaaa aaaaatcccg atcaacaagg
     3241 tcgggatttt tttttggaat gcgttatctt ttaattccag ccttgttcag ggtgcggata
     3301 taaatatctc tattgcgatt gtgttctgcc agcgttttag caaacaggtg atatcctgaa
     3361 ccatcgcctc tggcacacat gaaaatataa tcgtgatgtt catagtgcaa cactgcatca
     3421 atgtaggcga ttggaggcat gtgaatagga cccggaggaa gacccggata tttgtaagta
     3481 ttgtatggcg aatcgatctc caaatctgca aacagaattc ttttgatggt aaaatcaccg
     3541 atggcaaatt ttaccgtagg atcggcttcc agttt
//
LOCUS       DAOQOU010000245         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_18504_length_3550_cov_6.597711, whole genome shotgun sequence.
ACCESSION   DAOQOU010000245 DAOQOU010000000
VERSION     DAOQOU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18504_length_3550_cov_6.597711"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1230
                     /locus_tag="PLM90_13940"
     CDS             418..1230
                     /locus_tag="PLM90_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90533.1"
                     /translation="MLKSSSKLVTIILIAISSCSPPENSEPTNDYEKFVDGLVNLQEQ
                     AEFNFDSTIVGGNCCDFDFDTYFKYFDQLSVDSNWVLESHYRISAFRGHPVVASFARN
                     DTIAAEIRRELSLSRKGLLKGKEDYDLSLRLFGYMDSVGFLSAIHINSDSGYFQYLIF
                     ALFGGQYCLFGDANYADWEIITSRQKLKYFTELENDTYYRFSKSVKKQALKIDPEIKM
                     ETFPDSITFRIVTLDPWTGFVENKYSITKSWPHKLTQYEHKVLVKYDCGVRF"
     gene            1574..3298
                     /locus_tag="PLM90_13945"
     CDS             1574..3298
                     /locus_tag="PLM90_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HMZ90534.1"
                     /translation="MKAPKATDFQVIGDNQAALFTLKAHRGEGMLLLAMNWKTGKPPK
                     DFVGFSIEYKEPNGDKYYALKNRLSFLDDNGEPVEDSISSLQAPFQKFRWVHFPRNAD
                     LEGNFSYKVKPAFMNVNDELSYGESQIVEIALSRDTYPGKLNVCFTRGFVSSQAFVDK
                     YTAGGGIKALLPAKANLGLDFKPTHVKADEALPWMGFEAAREILNLLDEAIKDKDAEV
                     KVVAYDLNQPEVVNRLVKLGTRLQIVIDDSGDHGDDDSAETQAMKKLIKSTKGNVKRQ
                     HMGQLQHNKTIVVNGPKIKAAVCGSTNFTWRGFYVQANNAVIVKGAKAIKPFLEAFDN
                     YWQYDDVKGFGKTTSADWNDLQISGIDIQACFSPHASTNAKLDEIADDMENNTTSNLL
                     YSLAFLYQTTGSIRDAVTNLTANNKIFVYGISDKKVGGLDLQKPDGNVAPVYPSEIAK
                     NLPEPFKSEPTGGTGTRMHHKFVVIDFDKPTARVYLGSYNFSPTADLKNGENLMVIRD
                     RKIAVSYMIEALRIFDHYHFRIAAKEAKKSGKPLSLKRPPKKATEKAWWEEYYTDKRK
                     IRDRELFA"
BASE COUNT         1132 a          595 c          749 g         1074 t
ORIGIN      
        1 cctccatagt gattgctcat cgcctcagca ccatccgccg cgctgatcag atcatggtcc
       61 tcgacaaagg tgaagtcaaa gaaattggca cccacgacac cctgattgca ttggacggct
      121 actatgccaa gcttcatgcc atgcaatttg aagaggagcc gggatgagaa ataatatgtc
      181 ataattttag acaaatgtga catggtaaat gccggtttta tttgtcttct acattctagg
      241 actgaatatt tatgaagata gacaggagct ttcgcctcat attttgtgac atacctcata
      301 ttatcggttt tccaatagtc aatcattcta ccggcagtgt aaatttgtaa ggagtgagct
      361 gtgcgtaact ggtgataaat caatatttct tcccggttaa aaaactccaa aacacccatg
      421 ctcaaatcat cttctaaact tgttaccatc atcctcattg ccatctcctc ctgttcgccc
      481 cctgaaaact ctgagccgac aaatgactat gaaaaatttg ttgacggttt ggtgaattta
      541 caggagcagg ctgaatttaa ttttgatagt accattgttg gaggtaattg ttgcgatttt
      601 gactttgata cgtatttcaa atattttgat cagttgtcag ttgattcgaa ttgggttttg
      661 gagtcacatt atcggatttc tgccttccgg ggtcaccctg ttgtagcttc ttttgcaaga
      721 aatgacacca ttgcggcaga gataagacgt gaattatcat tgagcagaaa aggcttacta
      781 aaaggaaagg aagattacga tttgtcgcta cgcctctttg gttatatgga ttctgtaggg
      841 tttttgagcg caattcatat aaattcagat tccggctatt ttcaatatct gatatttgca
      901 ttgtttggag gtcaatattg tttatttgga gatgcaaatt atgcagattg ggaaatcatc
      961 acctcaaggc aaaagttgaa atatttcaca gagttggaga atgatacata ttacagattc
     1021 tccaaaagcg ttaagaaaca ggctctgaag atagaccccg aaattaaaat ggaaaccttt
     1081 cctgattcga taacttttcg gattgtcacc ctggaccctt ggaccggttt tgtagaaaac
     1141 aaatacagca tcaccaaatc ctggccgcat aaactaaccc aatatgagca taaggttttg
     1201 gtgaagtatg attgtggggt gaggttttag aaattcattt tttctaccgt ttcaaactct
     1261 tttgttgaaa attgctgctg caattctctt gttcaaatgt gacagcacaa aacacatgta
     1321 tttgttactg tgtcgcttga agcactttca attcatatat agttgttttt cgaaatggca
     1381 ttatttgtga catttgcaaa ttaacggttt tccgtgaatt tgaatgttaa agctttgatt
     1441 ttcgctcaat atcaattaaa ttaggtgaag catagaaatt aattatttat ggatcacatg
     1501 ttttacataa ttaaattgtg accgtattaa aatacctcat catcaataat caccattaaa
     1561 aaaattaatt caaatgaaag caccaaaggc aacggatttt caagttatcg gtgataatca
     1621 agccgctctg tttactttaa aagcacatag aggggagggt atgctccttt tagccatgaa
     1681 ttggaagaca ggtaaaccgc cgaaagattt tgtcggcttt tctattgaat acaaagaacc
     1741 gaatggcgac aaatattatg cattgaaaaa tcgcctgtca tttctagatg ataatggtga
     1801 gccggttgag gacagtatat catcattgca agcaccattt cagaaatttc gctgggtgca
     1861 ttttccaaga aatgcagact tggaaggaaa ttttagttat aaggttaaac cggcatttat
     1921 gaatgtcaat gatgaactga gttatggtga atcgcagata gtagaaattg cacttagtcg
     1981 tgatacgtat ccaggtaaat tgaatgtttg tttcactcga ggatttgtgt catcgcaagc
     2041 attcgttgat aaatatactg ccggtggtgg cattaaggca ctattgccgg caaaggctaa
     2101 tcttggacta gattttaaac ctacgcatgt taaagcagat gaagctcttc cttggatggg
     2161 atttgaggca gccagggaaa tattgaattt actggatgaa gcaattaagg ataaagatgc
     2221 cgaggtaaag gtagttgcat atgaccttaa tcagcctgaa gtagtgaata gactagttaa
     2281 gctgggcact agattgcaaa tagtcattga cgatagcggc gatcatggcg atgatgattc
     2341 ggctgaaacg caggcaatga aaaaattaat aaagtcgaca aaaggcaatg tgaagcgaca
     2401 gcacatgggt caacttcaac acaataaaac tattgttgta aacggaccaa aaattaaagc
     2461 agctgtttgt ggttcaacca attttacctg gagaggtttt tatgttcagg caaataatgc
     2521 cgttattgtc aagggtgcaa aggccataaa accatttctt gaggcatttg ataattattg
     2581 gcaatatgat gatgtaaagg gttttggtaa aactacttca gcagattgga acgaccttca
     2641 aattagtgga attgacattc aagcatgttt ttctcctcac gcatctacga atgcaaaatt
     2701 agatgagatt gcagatgata tggagaataa cactacttct aatttattgt attcgcttgc
     2761 ctttctttac caaacaaccg gttcaataag agatgcggta actaatttga ctgcaaacaa
     2821 taaaatattt gtatatggaa tttccgacaa gaaagttggc ggtcttgatt tacaaaaacc
     2881 tgatggcaac gttgcgccgg tttacccatc agagatagct aaaaatcttc cagaaccatt
     2941 taaatcggaa ccaacaggcg gcacagggac caggatgcat cataagtttg tagttataga
     3001 tttcgacaaa ccaacagcaa gagtatattt gggttcatat aatttttctc ccacggcaga
     3061 tttgaaaaac ggtgaaaatc ttatggtaat tagagataga aaaattgcag tttcctatat
     3121 gatagaggcg ttgcgaattt ttgatcatta tcattttcgg attgctgcta aagaagccaa
     3181 aaagtcaggg aagccattgt cattaaaaag accaccaaaa aaggccaccg aaaaagcgtg
     3241 gtgggaagaa tattacactg acaaaagaaa aattcgggat cgagagttat ttgcttaaag
     3301 ttaatgtgca gtctggagca atttgggaat agctgcttgg gagtgtactt gcgaagagat
     3361 aatagtttta attagcaaat ttgagaatta gcaaattagc aagtttgaga gttagcaaat
     3421 tagcaagttt gagagttagc aagttagcaa gtttgagagt ttgcaagtgt aggtgtttta
     3481 aggcatttcg ttaaaaggta tcaagtatca ggtatcaagt atcaggtatc aagtatcagg
     3541 tatcaagtat
//
LOCUS       DAOQOU010000246         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_18840_length_3516_cov_5.575267, whole genome shotgun sequence.
ACCESSION   DAOQOU010000246 DAOQOU010000000
VERSION     DAOQOU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18840_length_3516_cov_5.575267"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /gene="cmk"
                     /locus_tag="PLM90_13950"
     CDS             complement(<1..468)
                     /gene="cmk"
                     /locus_tag="PLM90_13950"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HMZ90535.1"
                     /translation="MKKIIIAIDGYAGCGKSTLARQLALKLGYTFLDTGAMYRAITLY
                     LLNNHINWHDEQALSNVFSQVKIHIATDQNGRTQTRLNGVLVEEEIRSMAVSNKVSEV
                     AAVSSIRRFLVAQQQQIGSEGGVVMDGRDIGTVVFPHAELKLFITASMEVRVAR"
     gene            complement(576..1418)
                     /locus_tag="PLM90_13955"
     CDS             complement(576..1418)
                     /locus_tag="PLM90_13955"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ90536.1"
                     /translation="MKKLLIITVAVTAAVAFSSCAKDRVYGCTDPFSINYNPNATDDD
                     GGCFAPSSQKRALMGDFTATWCPYCGQWGAPEFEEAINLSGANGIPLGIHNTDEITTN
                     ESTTLTDYYSAEGIVGGFPTLYVWNQASFSDGVPMAGAVSAEVGAGPAVAGAVAGFTD
                     NGSSMTINVSAQIFADVTGDYYVAAYLMENGLVYTQQIAGADPDPNFVHNHVIRASSN
                     GSAWGEQFVFGSASNGVVWTKSYQIEKNDAWKSENMYMVAIVWKYDSAEDKYTFVNAQ
                     ETHL"
     gene            1583..3424
                     /locus_tag="PLM90_13960"
     CDS             1583..3424
                     /locus_tag="PLM90_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HMZ90537.1"
                     /translation="MRLLKSLLTLTGLIFSLCVYAQPNIELETFATGLSNPVDIANAG
                     DDRMFVVERTGKIRIVEADGTVHADYFLDIHTQIESGYQEQGLLGLAFHPDYAVNGYF
                     YVYYTDTDGNTVVSRFSVSAGDPDYADAGSELVLLTENQPFLNHNGGCIKFGGDGYLY
                     IGLGDGGSAGDPGNRAQNTDLFLGKMLRIDVDGGTPYAIPDDNPFATATDTLPEIWAI
                     GLRNPWRFSFDALTKNLWIGDVGQNLYEEVDVELAGDGGHNYGWRCYEGLHEFNTAGC
                     DADTAEYIFPVLEYNHNATTGGFSITGGFVYRGSMYPGMYGYYITADYVSGNWWWIYA
                     DGGAPYTSLRMDDIQTSISCFGEDMNGELYCANLSSGIIYHIKDACGDFMAAAEVTDY
                     TCGVTDGAVDITITAGTAPYTYEWSSGETTEDISGITEGGTFTLTVTDASGCTRMITA
                     TVEELPGFIAVLNEDGGVLTATPGTSWQWYLNGEIIDGATSQSYTPTESGDYSCLVTD
                     ANGCSSMTPAFNVFVEITTAQWNIDFSIHPNPASDVIYLQITDAPCATNWYIADMTGN
                     VVWRSETIGKGNQSIAISLTSLANGIYLLRSDVGLAGSRVMKVSGEW"
BASE COUNT          971 a          748 c          806 g          991 t
ORIGIN      
        1 cctagccact cttacctcca tgcttgcggt aatgaaaagt ttgagttcgg catgcggaaa
       61 gactacggtg ccgatatctc tgccgtccat cactacccca ccctcgcttc ctatttgttg
      121 ttgttgggcc accagaaacc tgcgaatgct gctcactgct gctacctcac ttactttatt
      181 gctcaccgcc atacttcgta tttcctcttc caccaacacg ccgttcagcc gggtttgcgt
      241 gcgtccgttt tgatcggtag cgatatggat tttgacttga ctaaacacat tgctcagcgc
      301 ctgttcatca tgccagttga tatgattgtt gagcagatat agtgttatcg ccctgtacat
      361 ggctcctgta tcgaggaagg tatatccgag tttcaatgcc aattgtcttg ccagggtgct
      421 ttttccgcag cctgcataac cgtctatggc aatgatgatt tttttcacgg agctaagata
      481 agggttgacc ggtattcact tcatccccgc aagtgaaaat aaaaaaggtt gccccggtaa
      541 ggacaacctc tttttggcat gcaagaagtt tatgattaca aatgtgtttc ctgtgcgtta
      601 acaaaggtgt atttgtcttc tgcagaatca tatttccaaa cgatggcaac catatacatg
      661 ttttcagatt tccatgcgtc gtttttctca atttggtatg atttagtcca tacaacacca
      721 ttagaagcag aaccgaatac aaactgctca ccccaagcac taccgttaga agaagcgcga
      781 attacgtggt tgtgaacaaa gttaggatca ggatcagcac cggcgatttg ttgcgtgtaa
      841 acaagtccgt tttccatgag gtaagcagca acgtagtaat cgccggttac atcagcaaaa
      901 atttgtgcac ttacattgat ggtcatagaa gaaccattat ctgtgaaacc ggcaacagca
      961 cctgcaacgg caggacctgc accaacttca gcagaaacag ctcctgccat tggcacacca
     1021 tcagagaagc ttgcctgatt ccaaacatat aatgtaggga aaccgccaac aataccttca
     1081 gctgaataat agtcggtaag tgtagtagat tcgttagtag ttatttcatc agtattgtgg
     1141 atgcccagtg gaataccgtt ggcacctgac aaattgattg cttcctcaaa ttcaggtgca
     1201 ccccattgtc cgcaatatgg acaccaggtt gcagtgaaat cacccatcaa tgcacgcttc
     1261 tggctggaag gcgcaaagca accaccatcg tcgtcggtag cattagggtt ataattgatt
     1321 gagaaagggt ctgtacaacc gtaaacacgg tcttttgcac aggaggagaa tgctacagca
     1381 gcggttacgg ctactgtgat gatgagtaat tttttcatat gcctataaag ggttaggatg
     1441 aattattcct ctaagataca tcaaacttct gtatatacaa gtaattcttt ccacatttat
     1501 gaccaaattc ttacgttaat ttcgattgac atggggcgaa ggaaaggcgc ttgtgttacc
     1561 tttaacactt aaaatgacaa aaatgcgcct cctcaagagc ctgttaaccc ttaccggatt
     1621 gatattttcg ctatgtgtat atgcccaacc gaacattgaa ctcgaaacct ttgcaacggg
     1681 cttatctaac ccggtggata ttgcgaatgc aggcgacgac cgcatgtttg tggttgaacg
     1741 cacaggcaaa attcggattg tggaagccga tggcaccgtg catgccgatt attttcttga
     1801 tatccatacc cagatagaat cgggttatca ggaacagggt ttgttgggat tggcatttca
     1861 tcccgattat gctgtcaatg gatattttta tgtgtattac accgatacag atggtaatac
     1921 agtggtatca aggtttagcg taagtgccgg tgatccggat tatgcagatg ccggttctga
     1981 attggtattg ctcacagaaa atcagccatt tctcaatcac aacggaggct gcataaaatt
     2041 tgggggtgat ggctatttat acatcggatt aggtgatggc ggctcagcag gtgatcccgg
     2101 taacagagcg caaaataccg atctgttttt aggaaaaatg ctgcgtatcg acgttgatgg
     2161 tggaactcct tatgccatac cggatgataa ccctttcgcg acagcaacag atacgctccc
     2221 tgaaatttgg gctatcggct tgcgtaaccc ttggcgattt tcttttgatg cactcaccaa
     2281 aaatttatgg ataggtgatg taggtcaaaa tttatatgaa gaagttgatg tggaacttgc
     2341 aggcgatggc ggacataatt acggctggcg ctgttatgaa ggattgcatg aatttaatac
     2401 cgcaggttgc gatgccgata ctgctgaata tatctttccg gttttagaat ataatcacaa
     2461 tgctacaaca ggtggattca gtataacagg cggatttgtt tatcgcggaa gcatgtatcc
     2521 cggtatgtac ggttattata ttactgcaga ttatgtatcg ggcaactggt ggtggattta
     2581 tgccgatggc ggagcaccat atacaagctt gcgtatggat gatattcaaa cttccatttc
     2641 ttgttttggc gaagacatga atggtgaatt atattgtgca aacctgtcgt caggaattat
     2701 ttatcatatc aaagatgcct gtggcgattt tatggctgct gctgaagtaa cagattatac
     2761 ctgcggtgtt acagatggcg ctgttgatat tactatcaca gcaggaacag caccatatac
     2821 ctacgaatgg agctcaggtg aaacaacaga agatatttca ggtattaccg aaggaggcac
     2881 atttacatta actgtaactg atgcctccgg atgcacgaga atgattactg ctactgtgga
     2941 agaattaccg ggatttattg cggtattaaa tgaagatgga ggtgtactta ctgccacacc
     3001 cggcacttca tggcaatggt acttaaatgg cgaaattatt gatggtgcca catcacaatc
     3061 atacacacca acagaatccg gtgattatag ctgcctggta accgatgcaa acggatgcag
     3121 cagcatgaca ccggcattta atgtttttgt tgagataaca actgctcagt ggaatattga
     3181 tttctccata catccgaatc ctgcgagcga tgtcatttat ctccaaatca cagatgcacc
     3241 atgcgcaaca aactggtata tcgccgacat gacaggtaac gttgtttggc gttcggaaac
     3301 gataggaaaa ggaaatcaat ccatcgccat ttcgctgacc tcattagcta acggcatcta
     3361 tctcctacgc agcgatgttg gcttggcggg aagtagggtg atgaaggtga gtggtgagtg
     3421 gtgagttttg aattcctctg cgcctctgcg tctctgcgtg aaaaaaaaaa cagattgtaa
     3481 tgatgtatca aatcgcgaat ataccaaagc gcatca
//
LOCUS       DAOQOU010000247         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_18989_length_3499_cov_5.325494, whole genome shotgun sequence.
ACCESSION   DAOQOU010000247 DAOQOU010000000
VERSION     DAOQOU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18989_length_3499_cov_5.325494"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLM90_13965"
     CDS             complement(<1..905)
                     /locus_tag="PLM90_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HMZ90538.1"
                     /translation="MTYPEALEYLFTKLPMYQRIGAAAYKKDLSNTLALLKALGDPHK
                     KFTSIHIAGTNGKGSVTNMLSSICMEAGYRVGWYTSPHLEDFRERIKVNGHMCHEQFV
                     VDFVQRMEPHIEAIQPSFFELTVAMAFEYFALQEVEIAVVEVGLGGRLDSTNVITPIL
                     SVITNISFDHMQMLGNTLQEIAGEKAGIIKNHVPVVIGEYLPETLPVFEQKAAEMGSP
                     IFPAEENVAIEVLSQMPGKLEVNVSYLGQLIYPELICGLSGEYQVRNLRTVIQSTEVL
                     MAKGIELPEEALYEGLRKVIDNTGFA"
     gene            complement(997..1773)
                     /locus_tag="PLM90_13970"
     CDS             complement(997..1773)
                     /locus_tag="PLM90_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ90539.1"
                     /translation="MEKQGRTDTNNKVTSGFLTLGVTGGLLVAMLLLSFRPPNPPMVE
                     PEGLTVDFGQDLTGFGIEEPSSQISGGAPAHKPVVESAVTQQNESTITVPDKKNTTKP
                     TTQTTTTSTQQNTNTINSNELFDPSKLNNKGNSGSEGTAGGTGNEGSPDGDPNGYHGP
                     GAGRGDNGSVGWNLDGRSMKAPPTLQVDHNEIGDIRVKIYVDRNGKVTKAEYERSGST
                     ITDSYLISQSKNAAMKATFNVDKNAPEVQIGYMTFHFKLQ"
     gene            2116..3372
                     /locus_tag="PLM90_13975"
     CDS             2116..3372
                     /locus_tag="PLM90_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ90540.1"
                     /translation="MQTTTTSPFSGYQIFIIAIMAFLQFTIVLDFMVLSPLGAILMPE
                     LNITPSQFSHLVSAYAYSAFASGILSAGFADKFDRKKILLFFYVGFIGGTLMCGLAND
                     YHALLIARIITGLFGGVIGSVVFAITTDLFAMQVRGRVMGFIQMSFAASQVMGLPVGL
                     YLANKMNWHFPFYLIVAIGSIVFFIISIKMKPVNAHLAIKRDISPLKHLLKTLSRKRY
                     ITGFSATVLLATGGFMLMPFGSAFAVNNLGLTMEQIPLVYMVTGICTLIAGPFIGKIS
                     DRVGKWSVFLAGTILTMILVLFYCNLGITPLWLAITVNSILFIGITSRMIASSALMSG
                     VPDPQDRGSFMGLNSSISQLAGGMAASLAGLIVVQEDNGHLLHYPILSYVVVFAMMIT
                     LVMMYRIHKMVTPTITPSINPEISAS"
BASE COUNT          944 a          749 c          696 g         1110 t
ORIGIN      
        1 gcaaaacctg tgttatcaat cactttcctc aatccttcat atagtgcttc ctcaggtaat
       61 tctataccct tagccatcaa cacttcagtg gattgaataa ctgtccgcaa atttctgacc
      121 tgatattctc cggataagcc gcatatcagt tcagggtata tgagttgacc gaggtagcta
      181 acatttactt ctaatttacc gggcatttga gatagcactt caatggctac attttcttct
      241 gcaggaaata ttggtgatcc catttcagca gctttttgct cgaatacggg caaggtttca
      301 ggcaagtatt cgccaattac taccggaaca tgatttttga tgattccggc tttttctccg
      361 gctatttcct gcaaggtatt gcctagcatt tgcatgtgat cgaaactgat attggtgatc
      421 acagataata ttggtgtaat tacattggta gaatccaaac gcccgcccag acctacttcc
      481 acaacagcta tttctacctc ctgcaaggca aaatattcaa atgccatggc tacggtcaat
      541 tcaaaaaacg aaggttgaat agcctcaatg tgcggttcca ttcgttgcac aaaatcaacc
      601 acaaattgct catggcacat atggccattt accttaatac gctcccggaa gtcttcgaga
      661 tgtggggacg tataccaacc aacacgataa ccggcttcca tgcaaatact cgatagcata
      721 ttagtaacag aacccttgcc gttagtgcct gcaatatgaa tggacgtgaa ttttttatgt
      781 ggatctccaa gtgctttgag gagtgctaac gtgtttgaaa gatctttctt atacgctgcc
      841 gcaccgatcc gttgatacat cggcaatttt gtgaatagat attccagtgc ttccggatac
      901 gtcataaccg ctgcaaatag acgtaaaaag gcttgaaatc gaaagtattt gcacaaaaaa
      961 agcctcccga aggaggcttc aattatttca aactgtttat tgcaatttaa agtggaaggt
     1021 catgtaaccg atctgcactt caggggcatt tttatcgacg ttaaacgtag ctttcattgc
     1081 agcgttttta ctttgactga tcagataact atctgtgatg gttgagccac tgcgttcgta
     1141 ttctgctttg gttacttttc catttctatc cacatatatc tttacgcgaa tatcgccaat
     1201 ctcattatgg tctacttgaa gggtaggggg cgctttcata ctgcgaccgt cgaggttcca
     1261 acccacagaa ccattatcgc cgcgacctgc accgggacca tgataaccat tgggatcacc
     1321 atcaggactt ccctcgttac cggtaccacc tgcagttccc tcgctaccgg aattaccttt
     1381 gttattcagc ttgctagggt caaataactc gttagagttg atggtattgg tattctgctg
     1441 cgtagaggta gtagtagtct gcgtagtagg cttagttgta ttttttttgt cagggacagt
     1501 gatagtagac tcattttgct gcgttacagc cgattctacc accggtttat gagccggagc
     1561 acccccggaa atttgagacg aaggctcttc aatgccaaat ccggtcaaat cctgcccaaa
     1621 atctactgta aggccttcag gctctaccat aggcgggttt ggcggtctga aactcaaaag
     1681 taacattgcc acgagtaatc ctcccgtaac accaagcgtt aaaaatccgc tcgttacttt
     1741 gttgttagtg tctgttctac cttgcttttc catatgcttt cggttttaga tatgatgcaa
     1801 tgatgatgta taatccacaa aattcattcc tatcttaccg caagtcaatg ttttaaaacg
     1861 gttttcgacc gaaggatagt ataggatttt acaaaattaa gctactacac atctagtaca
     1921 agcccgactt caacgtaata aatcgacaat gtagctttaa atagacgagg cataacaaaa
     1981 attgcagagt gcttgcacac attttcatgt gtatacatga ttttttcatg ttttcacttg
     2041 gatttaatat tgcagagagg tttagatgaa ctaaaagcta ttgattagcc ttagcatctt
     2101 ttcaggcaac tacttatgca aacaactact acttctccgt tttcggggta tcagatcttt
     2161 attattgcca taatggcttt tttacagttc accatagtcc tcgattttat ggttttatcg
     2221 ccgttgggag cgatcctgat gccggaactc aatatcaccc cttctcagtt tagtcatctg
     2281 gtttccgcct atgcatacag cgcatttgcc tccggaattt taagcgcggg ctttgctgat
     2341 aagtttgaca gaaagaaaat actattattt ttctacgttg gatttattgg tggcaccttg
     2401 atgtgcggat tagccaatga ttatcacgcc ttattgatag cgcgcatcat tacaggttta
     2461 tttggcggtg taatcggatc tgtggtcttc gcaatcacca ccgatctatt cgccatgcaa
     2521 gtacgtggtc gtgtaatggg attcattcaa atgtcgtttg ccgccagtca ggtaatggga
     2581 cttcccgttg gattatatct tgccaataaa atgaactggc attttccctt ttatctcatc
     2641 gttgctattg gcagtattgt gttttttatt atttccatta aaatgaagcc ggtaaatgca
     2701 catttggcaa ttaagcgcga tatttctcct ttaaaacatt tactcaaaac actttccaga
     2761 aagcgatata tcacaggttt ttcagcaaca gttttgctgg caacaggtgg ttttatgctt
     2821 atgccttttg gtagtgcctt cgcagtaaat aatcttggac tgaccatgga acaaatacca
     2881 ttggtttata tggtcaccgg aatttgcaca ttgatcgcag gaccatttat tggtaaaata
     2941 agcgataggg tagggaaatg gagtgtgttt ttagccggaa caatactgac aatgatcctg
     3001 gtattatttt attgcaatct gggcatcaca ccactgtggt tggctattac ggtaaacagc
     3061 attttattta ttggtattac ttcccgcatg atagcctctt cggcattaat gtcgggagta
     3121 cctgatccgc aagaccgcgg ttcatttatg ggattgaatt caagcatttc tcaattagca
     3181 ggaggtatgg ctgcttcatt agcagggttg atagtagtac aagaagacaa tggtcactta
     3241 ttacattacc cgatattaag ttacgttgtt gtttttgcca tgatgatcac tttagtgatg
     3301 atgtatagaa tacataaaat ggtgacacca actatcacac cttccatcaa tccggaaatt
     3361 tctgcatctt agtacattat tttaatacga acaccatgca agttaaatca ctattatccg
     3421 cagctttggt aagtgcatta gtacttactg cttgtaattc cacaaaacaa gcaggcacaa
     3481 ctccggctat tacctacag
//
LOCUS       DAOQOU010000248         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_19167_length_3482_cov_6.231981, whole genome shotgun sequence.
ACCESSION   DAOQOU010000248 DAOQOU010000000
VERSION     DAOQOU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19167_length_3482_cov_6.231981"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLM90_13980"
     CDS             complement(<1..734)
                     /locus_tag="PLM90_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HMZ90541.1"
                     /translation="MRIPFLFFAIAMLFIMGCNGDWMDYSPADMYSASIDYDDADAPL
                     RLDVAAVTIASDKDNKSAVLADMQSMTEQIMLEHPEVDVIEFGELILGWYYDSDSEEA
                     YQRAVSEPIPGSSFYVLQELAISHQINLVYGAAEVDTVTNKIYNSQVLIKKTGDYVVY
                     RKRNLNDLDIANGFSAGSEFVSTTIDGVNVAMFICSDMQSNQITEEMADADVDVILQS
                     VTSTTDMSADISYVGMQMNKWIVFANR"
     gene            complement(873..1376)
                     /locus_tag="PLM90_13985"
     CDS             complement(873..1376)
                     /locus_tag="PLM90_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90542.1"
                     /translation="MKTKVHITVIYEATLERAFKTPMLCDVTKVHTGYGLMPKIIDVV
                     NDSDWGKPGSSKGVIAAKSISFKGGLASTDHVLARVENDYWHICVDDFQAWMLGLYKF
                     EGIWETTQLEKGRVQVDYTYHLHANQPLFYPFVWLFSKLFWKRYMVHVTENIRSLIAN
                     DEPYMYN"
     gene            complement(1414..1866)
                     /locus_tag="PLM90_13990"
     CDS             complement(1414..1866)
                     /locus_tag="PLM90_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMZ90543.1"
                     /translation="MKSIFSPEVSTEVIARIEKLTPESKPLWGKMSVDKMLAHCNVTY
                     ELIYDNKHPKAGAFKQFLLKLFVKNAVVSEKPYKKNSPTAPEFKIVDPRIFADEKKRL
                     IDYINKTQQLGADYFDGKESNSFGKLNSTEWNIMFYKHLDHHLGQFGV"
     gene            2021..3409
                     /locus_tag="PLM90_13995"
     CDS             2021..3409
                     /locus_tag="PLM90_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90544.1"
                     /translation="MPRIQYLATCLLIVLVLPCSAQFSTIFDFTGDTTGSYPYYCKLL
                     EVDGQLYGTTANGGAFYDGTIFKINPDGSGFQKLIDFNEETTGSIGESALIYDGEFLY
                     GTARSGGDFNMGTIYKIKPDGSEFSKLHDFIGSDDGINPYGSLFFDGSFLYGMASGGG
                     AGGHGTIYKLKRDGTDFSSILAFDFGTSGNGPNGALISDGTYLYGMATSGGTGLYGTV
                     FKIKPDGSDFSVLTSMSDDPNGAYNYGSLVYDGSYLYGMTNNGGELNRGTIFKISTDG
                     TDYQKLHDFDDTTGAQPLGSLTLVGSTLYGLAELGGEFGKGTMFKLETDGSNFEKLYD
                     FNGINGELPISTLTNYNGALFGMTNTGGSKSQGVIFKYGEFTSEVTQYEHGKIHMYPN
                     PATEVIHIDPELLVYDKELPVQIINTSGQCVLATAISGSGTISVKSLPSGIYQLIISA
                     GGALYAETFVKQ"
BASE COUNT         1074 a          721 c          624 g         1063 t
ORIGIN      
        1 cggtttgcaa atactatcca cttattcatt tgcataccaa cataactaat atcggcactc
       61 atgtcggttg tagaggtcac cgattgcaaa ataacatcca catcagcatc tgccatttcc
      121 tcggtgatct gattactctg catgtcggag caaataaaca tagcaacgtt cacaccatca
      181 atagttgttg atacaaactc acttcctgct gagaatccgt tggcaatatc caaatcattc
      241 aaattgcgtt tcctatatac tacataatct ccggtcttct ttatcaacac ttgcgaatta
      301 tatattttat ttgtaacagt atctacctca gcagcaccat aaaccaggtt tatttgatgc
      361 gaaatggcca attcctgcaa tacataaaac gagcttccgg gtatcggttc actaacagca
      421 cgttgatagg cctcttcact gtcgctatca taataccaac ctaaaatcaa ctcaccaaat
      481 tcaatcacat ccacctcagg atgctccagc attatttgtt ctgtcatact ctgcatatcc
      541 gccaagacag ctgatttatt atccttgtca gaggctatag tcactgcggc aacatcaagc
      601 cgaaggggag catctgcatc atcataatca atagatgcgc tatacatatc tgccggagaa
      661 taatccatcc aatcgccatt gcatcccatg ataaacagca tagcaattgc gaaaaataaa
      721 aaaggtattc gcatacttat attaaaaact tatgattttg aaatatgggc gtgctctatg
      781 aaggtaacga ttgaatcgct ttgcaaaaat aacgagaatc gtggtaattt atttcatact
      841 acctcaattt ataattgtta taaactggga atttagttat acatataagg ctcatcattt
      901 gctattaagc tgcgaatatt ctcggtaacg tgtaccatat atcgcttcca aaacaactta
      961 gaaaacaacc atacaaacgg ataaaaaagc ggttgatttg catggagatg ataggtataa
     1021 tccacctgca ccctaccctt ctctaattgc gtagtttccc aaataccttc aaacttgtac
     1081 aaaccaagca tccaggcttg aaaatcatcc acacaaatat gccagtaatc attttccacc
     1141 ctggccaata catgatctgt agatgcaagt ccgcctttaa aacttataga tttggcagcg
     1201 atgacacctt ttgaagaacc gggtttaccc cagtcgctat cattaaccac atcaattatt
     1261 ttaggcatca atccataacc tgtgtgcact ttcgttacat cgcataacat tggtgttttg
     1321 aaggcgcgtt ctaaagtggc ttcatatata acggtaatat gaactttcgt tttcatgacg
     1381 caacctttat ctgtgataac ataattaact gaatcaaacc ccaaactgcc ccaagtgatg
     1441 atcaagatgc ttgtagaaca tgatattcca ttctgtagaa ttcaattttc caaaggaatt
     1501 cgattctttt ccatcaaaat aatctgcacc taattgttgc gttttattga tatagtcgat
     1561 cagtcgcttt ttttcatcag caaaaatccg tggatctaca attttgaatt caggtgctgt
     1621 tggtgaattc tttttataag gcttttctga tactacagca ttttttacga acaacttcaa
     1681 cagaaactgc ttaaatgcac ctgctttcgg atgcttattg tcataaatca attcgtacgt
     1741 gacattgcaa tgagccaaca ttttatctac cgacatcttt ccccatagtg gcttcgattc
     1801 cggtgtcaat ttttcaattc tggcaataac ttcagttgaa acttcaggag agaatatact
     1861 tttcataact ataattggcg atttaatatt ttcagcaaag ttaaggcaat tcacaaaact
     1921 gtcaataatt gatttaaaag atcaaaaatt gaaatgcccc tcccccaatg tcggtattgt
     1981 gccttcattc aaatcgattg acctttgtaa aaacgacatt atgccacgta ttcaatacct
     2041 tgcaacttgc ctgcttatag tcttagtgct accatgtagt gctcagtttt cgaccatttt
     2101 tgattttacc ggtgacacca caggatctta tccatattac tgcaagttac tagaagttga
     2161 tggtcaatta tatggcacta cagcaaatgg aggtgcgttt tatgacggta cgatatttaa
     2221 aataaatccg gatggctccg gctttcaaaa attgatagac ttcaatgaag aaactaccgg
     2281 ctcaattggc gaaagtgcgc ttatttacga tggggaattt ttatacggca cagcacgttc
     2341 cggtggcgat ttcaatatgg gtactattta taaaatcaaa cctgatggca gtgagtttag
     2401 taagttacat gatttcatag gaagtgatga tggaataaat ccttacggca gcttgttttt
     2461 cgatggctct tttctttatg gaatggcaag tggtggaggc gcaggaggtc acggtaccat
     2521 ttataaactt aaaagagacg gcaccgattt ttcctcaatt ttagcatttg attttggcac
     2581 ctccggcaat ggacccaatg gtgcacttat ttctgatggc acctatctat atggcatggc
     2641 cacatcgggt ggtacagggc tatatggaac agttttcaaa atcaaacccg acggcagcga
     2701 tttttcagtg ttgacatcta tgtctgacga tccgaatggt gcctataact atggttcatt
     2761 ggtgtatgat ggcagctatt tatatggcat gacaaacaat ggaggtgaat taaaccgggg
     2821 aaccattttt aaaatctcaa cagatggcac cgattatcaa aaactacatg attttgacga
     2881 cactacaggt gcacagccat taggctctct tactctggta ggatcaactt tatacggatt
     2941 ggctgaatta ggcggagaat tcggaaaagg caccatgttt aaactagaaa cagatggcag
     3001 caattttgaa aaattatatg atttcaatgg tataaacggc gagttgccaa ttagcacatt
     3061 gacaaattat aatggtgcac ttttcggcat gacaaataca ggaggttcaa aaagccaggg
     3121 tgttattttt aaatatggtg aattcacctc agaagtgact cagtatgaac atggaaaaat
     3181 ccatatgtat cccaatcctg caaccgaagt aatccatatt gatccggagc ttctggttta
     3241 tgataaggaa ttgcctgttc aaataataaa tacctccggt caatgtgttt tggcaactgc
     3301 tatatcaggt tccggtacaa tttctgtgaa aagtctccca tcagggatct atcagctcat
     3361 tatatccgca gggggagcat tgtatgctga aaccttcgtc aaacaataac atttacacca
     3421 tgtacttttg ttgaagtagc aattgggaca aaacagttcc aaagttttca caatacatta
     3481 ca
//
LOCUS       DAOQOU010000249         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_19328_length_3465_cov_5.181525, whole genome shotgun sequence.
ACCESSION   DAOQOU010000249 DAOQOU010000000
VERSION     DAOQOU010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19328_length_3465_cov_5.181525"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLM90_14000"
     CDS             complement(<1..484)
                     /locus_tag="PLM90_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HMZ90545.1"
                     /translation="MNKVFKNADEAIADIFNGAILMLGGFGLCGIPENCISALVRKKV
                     NGLTCISNNAGVDDFGIGLMLQQRQVKKMIASYVGENAEFERQLLSGELEVELIPQGT
                     LATRCMASGYGMPAIFTPAGEGTEVADGKETRVFNGKTYLLEYAFDADYAIVKAWKGD
                     T"
     gene            complement(481..873)
                     /locus_tag="PLM90_14005"
     CDS             complement(481..873)
                     /locus_tag="PLM90_14005"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HMZ90546.1"
                     /translation="MRLTLLICSALLMMTFACKPKDPFVVNDQMLNSVPDSVIVVDSM
                     INILTDIQLAEAYVNEMRSDSIPKEDRLKQYYSEIFSMHHIDGTSYKRSYDYYVGNPI
                     LMNHIYVKVTEKLNLMESEHATVKKNVK"
     gene            909..1562
                     /locus_tag="PLM90_14010"
     CDS             909..1562
                     /locus_tag="PLM90_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMZ90547.1"
                     /translation="MSSTYHEIKTFLDARKVQLIAVSKTRPIAQLMPLYEAGQRDFGE
                     NRVQEMREKHELMPADIRWHLIGHLQRNKVKYIAPWVHMIHSVDSADLLKEIDRQAVT
                     CNRVIPCLLQIYVATEETKFGLDEPELMALLSSEAFHAMHNIQICGLMGMATNTENQE
                     QIRKEFATLHAIFERTKARFFAQDNNFKELSMGMSSDYNIAVEQCSTMVRIGSLLFN"
     gene            1592..2632
                     /locus_tag="PLM90_14015"
     CDS             1592..2632
                     /locus_tag="PLM90_14015"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HMZ90548.1"
                     /translation="MISGPKALVTIAFAYSLLSACAYMPVRATFTEAEIPAAPDFANP
                     DLWSALPTRHDSADHIPNDNIFPVVDGQADAAVDVFFIYPTQFFSRSEWNASLDDKKL
                     NATIDARAITHQASVFNGSCRVYVPRYRQATFNAYFSLTNPDAKKAFELAYSDIKAAF
                     EYYLEHYNNGRPIIIAGHSQGTTHAKWLLRDYFDGKPLVDKLVAAYLIGMPVYENDFT
                     QIAPCDSAGQTGCYVSWRSYLEGSEQKPKLIAADRDKIVVHNPLTFSRTTDFISSDHN
                     IGGLDRDANTLIPNACSAQIHEDIVWVSRPDIRGKAFIPENLHPADYNLYWLTIRNNV
                     QMQVNNYLQTHK"
     gene            complement(2633..>3465)
                     /locus_tag="PLM90_14020"
     CDS             complement(2633..>3465)
                     /locus_tag="PLM90_14020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HMZ90549.1"
                     /translation="AAPFNYEVIFTEYRDHAALIAKNAVQQGFRRFVILGGDGTANDV
                     INGLCQSGISTEMFTVAMIPAGTGNDWVRTIGKPDFAMIVEDLIRHETMKHDIANVTY
                     VNAGKEAMRYFINMAGLGFEGAVAKKIYERSSKWQGGKMQYQLAILSTLFVYKHTQLE
                     IKVDGNSSEMQFLSAAIGSGKFNGGGMKQLPAAIFNDGILDLTVIATMSKMKMVISLP
                     KLKTGAHIGMQEVQTFKGKIIEMNSNPPVHMDADGEYLGQTPLRFQVASEQINVIKWR
                     "
BASE COUNT          984 a          750 c          691 g         1040 t
ORIGIN      
        1 tggtatcgcc tttccatgct tttacaatag cgtaatctgc gtcaaaggca tattccaata
       61 aatatgtttt accgttaaac acccgtgtct ccttgccatc cgctacttct gtgccttcac
      121 ctgcaggagt aaatatagca ggcatgccat atcctgaagc catacatctg gtagcgagag
      181 taccttgagg aattaattca acctccagtt cgccactcag taattgtcgc tcaaattcag
      241 cattttcacc tacataacta gcgatcattt tcttgacctg gcgctgctgc aacattaaac
      301 cgatcccaaa atcatcgaca cctgcattgt ttgaaatgca tgttaaaccg ttgacttttt
      361 tacgcaccaa tgcggagatg caattttccg gaattccaca taatccaaat cctccaagca
      421 tgagtatagc gccattgaaa atatcggcta tggcttcatc agcatttttg aatactttat
      481 tcattttaca ttctttttta cggtagcatg ttcactttcc atcaagttta atttttcagt
      541 caccttcaca taaatatgat tcatcaaaat tggattgcca acataataat catagctgcg
      601 tttatatgaa gtgccatcaa tatggtgcat ggaaaatatt tcactataat attgttttaa
      661 gcgatcttcc tttggtatag agtctgaacg catttcgttc acatatgcct cggctagctg
      721 gatatcggta aggatattga tcatgctatc gacaacaatt acagaatcag gaacactgtt
      781 tagcatctga tcatttacta caaaaggatc cttgggtttg caggcaaagg tcatcatcaa
      841 aagcgcagaa catatcaaca aagtcaatcg catcactgca atggtttatt tttgcgctaa
      901 gttagccaat gagcagcaca tatcacgaga tcaaaacctt tctggatgcc cgaaaggtgc
      961 aattgatagc ggtttctaaa accaggccaa ttgcacaatt gatgccattg tatgaggcgg
     1021 ggcaacgcga tttcggtgaa aaccgggttc aggagatgcg cgaaaaacat gaactgatgc
     1081 ctgccgatat ccgctggcat ctgatagggc atctccagcg caataaggtg aaatatatcg
     1141 ccccctgggt gcatatgatc catagtgtcg acagtgccga tttgctgaag gagatcgatc
     1201 gtcaagctgt tacgtgcaat agggtaattc catgcctttt gcagatttat gtggcaactg
     1261 aggaaacaaa atttggcctc gatgagcctg aactaatggc tttattgtca tcagaagctt
     1321 ttcatgctat gcataacatt caaatttgtg gtttgatggg catggcaaca aatactgaaa
     1381 atcaggagca gatacgcaag gaatttgcca cgttacatgc catatttgaa cgcacaaaag
     1441 cgcgtttttt tgctcaggat aacaacttta aggagctgag catgggcatg agcagcgact
     1501 ataatattgc cgtggaacaa tgttccacga tggtgcgaat tggcagcctt ctttttaatt
     1561 aggcgcactt tttgcttgta tctttgcgcc tatgatctcc ggtcccaaag cattggttac
     1621 aattgcattt gcatattctt tattatcagc gtgtgcatat atgccggtaa gggcaacctt
     1681 tactgaagct gaaatccccg ctgcgcctga ctttgcaaac cctgatttat ggagtgcttt
     1741 gcccacgaga catgattctg ctgaccatat tccaaatgac aatatctttc ctgttgttga
     1801 tggacaggct gatgcagctg tagatgtatt ttttatttat cctacacagt ttttttcccg
     1861 cagtgaatgg aatgcttctc ttgatgataa gaaattaaat gccaccattg atgcgcgagc
     1921 aattacacat caggcgagtg tgttcaatgg cagttgccgc gtttatgttc cacggtatcg
     1981 acaagcaaca ttcaatgcct atttttcact taccaatccg gatgcgaaaa aagctttcga
     2041 attagcctac agcgatatca aggctgcctt cgaatattat cttgaacatt acaacaatgg
     2101 aaggcccatc attattgccg gtcatagtca gggcaccaca catgcaaaat ggttgttacg
     2161 tgattacttt gatggcaaac cgctggttga caaacttgta gccgcatatt taattggcat
     2221 gcctgtgtat gaaaatgatt ttacacagat agcaccttgt gatagcgcag gtcaaacagg
     2281 ctgctatgtg agttggagaa gctatcttga aggcagtgaa caaaaaccaa aattaattgc
     2341 tgctgaccgc gataaaattg tagtgcataa tccgcttaca ttcagcagaa caactgattt
     2401 catcagcagc gatcataata tcggcggctt ggatcgcgat gccaacactt taataccgaa
     2461 tgcctgcagt gctcagatac atgaagatat tgtttgggta agcagacctg atattcgggg
     2521 gaaagcattt attcctgaaa atttgcatcc tgcagattac aatttatatt ggcttacaat
     2581 tagaaataat gtgcagatgc aggtaaacaa ttatcttcag actcataaat gatcagcgcc
     2641 atttaatgac gttgatctgc tccgatgcca cttgaaagcg aagtggtgtt tgcccaagat
     2701 attcgccatc agcatccatg tgaacaggtg gattgctatt catctcaatg attttccctt
     2761 tgaacgtttg cacctcctgc ataccaatat gtgcacctgt ttttaatttt ggcaaactaa
     2821 ttaccatttt cattttactc atggttgcaa ttacggtaag gtcaagtatt ccatcattaa
     2881 aaatggctgc cggtaattgt ttcatgccgc caccattaaa tttaccgctg cctatagctg
     2941 cagacaaaaa ctgcatttca gatgaattgc catcaacttt tatttcgagt tgtgtatgct
     3001 tgtacacaaa gagcgttgat aaaattgcca attgatattg cattttaccg ccctgccatt
     3061 ttgatgagcg ctcatagatc ttttttgcaa ctgcaccttc aaaaccgagt ccggccatat
     3121 tgataaaata ccgcatggcc tcttttccgg cgtttacata ggtaacattg gctatatcat
     3181 gtttcatggt ttcatgtcga ataagatcct caacaatcat tgcgaaatca ggcttaccaa
     3241 tcgtgcgaac ccaatcattc cctgttcctg caggtatcat ggcaacggtg aacatttctg
     3301 ttgagatacc tgattggcag agtccgttta tgacatcatt tgcagtacca tctccaccaa
     3361 gtattacaaa gcgacgaaag ccttgttgaa cggcattctt ggctatcaac gcagcgtgat
     3421 cacggtattc agtaaatatt acttcatagt taaatggagc tgcat
//
LOCUS       DAOQOU010000250         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_19375_length_3461_cov_4.826776, whole genome shotgun sequence.
ACCESSION   DAOQOU010000250 DAOQOU010000000
VERSION     DAOQOU010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19375_length_3461_cov_4.826776"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLM90_14025"
     CDS             complement(<1..902)
                     /locus_tag="PLM90_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HMZ90550.1"
                     /translation="MEIVLQVLFAVVFGVSFYLAGRKFVAIRRNILLGRKESLTDQPA
                     RRWKHMVLFALGQKKMFRKPLPAVLHLFIYVSFVIVNLELIEIAIDGVTGNHRTFAPY
                     LGGFYTFVISLIETLSALALVATLVFLWRRNVLKVKRFTQPELKGWPFKDANFILGME
                     LILVTAILTMNGADYRLQQLGAAHYVDTGTLLVGQFTSQLFMHMGTASLEHVERIAWW
                     VHILGILFFLNYIPYSKHLHIILAFPNAYYTRFAVAGKMENMPVIANEVKSMLDPSAT
                     VTEIPDPPKTFGAKDVTDLTWKHLL"
     gene            969..3413
                     /locus_tag="PLM90_14030"
     CDS             969..3413
                     /locus_tag="PLM90_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ90551.1"
                     /translation="MPKTLLIVLLAAICFSADAQQSSPGSCIGLSAYHAMNDAAPGEQ
                     IPVYIKGDVPALAALIKQYGGTFKFAAGDIISATLDRAAIRAINESGLAVRIEANANK
                     GNLLNDVMVLHNNVDSAFYGYWPLEQAYDGTGVVVGIIDAPFDLDHGDFTDADGNSRI
                     KYVWDQNITTGAPPTPYGYGVECDSAMIADGTCPSVDDDPLHYSHGSGVTGVAASSGM
                     ASNHYRGVAPNADLILVSMDFNNDYETNATDAIAYIYAKAAEMGKPCVINTSFGSYAG
                     SHDGSDLMSQTIDALITASPGRALVAAAGNAGNNAFHLGYDVTATEQFTWFKKLSYMN
                     LVYFQLWADTTDFNQVDFRLSVDEPAGFTSLGSTPYFNMLSDFDFTGDIIDSVTYSIP
                     GGGNITIYAQLDNDKYFLEFVATPSVPGNYWRLSTVGSGHFDIWSAESTTGFSNYVTT
                     GLPSAVVLPEIVNYRLPDTDQTIVSSFQCSDHVITVGSYVNRDSMTNFYLENPPFFDV
                     VGALFYSSSHGPTRDGRIKPDICAPGARVLSTASDTLTEWLALLDAATYVSADAQHYL
                     YNGTSFSSPAVAGIAALYLQKNPNANAAEIKDAILSQARKDDFTGAALPNNLWGYGKA
                     DAFRTLTGDWGCAADDYTEPPVDLQVLATTPTKAMLDWDIIPNAAGYQIWYKSSGGSW
                     NKLKSPSHTKTISGLTPNTTYNAKVRSYCTGFGFSDFSTTITFTTPPLREDISSEMLI
                     NVYPNPASNILHVDGVDAGSSIVIVNMLGQVIYNQTTTNAETNQIDIHDWAEGMYRLL
                     INEHDNYTLHTFIVAR"
BASE COUNT          972 a          783 c          758 g          948 t
ORIGIN      
        1 agtaaatgtt tccaggtgag gtcggtcaca tctttggcac caaaggtttt tggcggatcc
       61 ggtatttctg taactgttgc cgaggggtct agcatcgatt tcacctcatt ggcaataacg
      121 ggcatgtttt ccatttttcc cgccacagca aatctggtat aataagcatt ggggaatgct
      181 aaaataatgt gaagatgttt cgaataagga atgtaattca ggaagaataa aatacccaga
      241 atatgtaccc accatgcaat acgttcaaca tgctctaatg atgcagtgcc catatgcata
      301 aaaagttgac tggtgaattg accaaccaat aaggtgcctg tatctacata atgcgctgca
      361 cctaattgtt gaaggcggta atcggctcca ttcatggtga gaattgccgt aactaatatc
      421 agctccattc ccaaaataaa attggcatct ttaaacggcc agccttttaa ttcgggctgc
      481 gtaaatcgct ttaccttgag cacatttctt ctccatagaa aaacaagcgt tgccactaat
      541 gccaatgccg ataatgtttc gatcaggcta ataacaaaag tgtaaaatcc acccagatac
      601 ggtgcgaaag ttctatgatt gccggttacg ccatcgattg caatctcaat taattcgagg
      661 ttgacgatta caaaactcac atagataaat aggtgtaata cggcaggaag cggcttgcga
      721 aacattttct tttgtccaag tgcgaaaagc accatatgct tccatcttct ggccggctga
      781 tctgtaagcg attcttttcg ccccaataat atattcctgc ggatggcaac gaattttctc
      841 cccgccaaat agaaggacac accaaacacc actgcgaaaa gcacctgcag aactatctcc
      901 atgcaatact tgtttatacg gttgctaaat taagcgtatt tttaggactg taaaaaggga
      961 ttatacacat gcctaaaacc ttactcatag tattgttagc cgcaatatgt ttttcggcag
     1021 atgcgcaaca atcttccccc ggcagctgta tcgggctttc agcctatcat gccatgaatg
     1081 atgcggctcc cggtgaacaa atccctgtct acatcaaggg cgatgtgccg gctcttgcag
     1141 cactcatcaa acagtatggc ggcaccttta agtttgctgc cggagatatc atttcggcta
     1201 ctttagaccg cgctgcgatc agggctatca atgaaagtgg cctggcagtc aggattgagg
     1261 ccaatgccaa taaaggcaac ctgctgaatg atgtgatggt gctccacaac aacgtagatt
     1321 cggcctttta cggctattgg cctctggaac aggcctacga tggcacggga gttgtggtag
     1381 gtatcataga tgcccctttt gaccttgacc atggcgactt taccgatgcc gacggcaata
     1441 gtcgcattaa atacgtttgg gaccagaata tcacaaccgg agcccctcct accccttatg
     1501 gatatggtgt tgagtgcgac agcgctatga ttgctgatgg cacctgtcca agcgtggatg
     1561 atgacccttt acattattcg cacggaagcg gcgttaccgg ggttgcggca tccagcggca
     1621 tggcatcgaa ccactaccgc ggagtagctc caaatgctga cctgatcttg gtttcaatgg
     1681 attttaacaa tgattatgaa accaacgcaa cagatgccat agcttatatc tatgccaaag
     1741 ctgctgaaat ggggaaaccc tgcgttatta atacctcttt cggctcttat gccggttctc
     1801 acgatggttc cgacctgatg agtcagacca tagatgcctt gataacagcc tctccgggac
     1861 gcgcacttgt tgctgctgca gggaatgcag ggaataatgc cttccacctg gggtacgatg
     1921 ttactgccac tgagcaattc acctggttta agaaattgag ttacatgaac ctggtttatt
     1981 ttcagttgtg ggcagatacc actgatttta atcaggtaga ttttcgctta agtgttgatg
     2041 agcctgccgg atttacatct ttgggaagta ccccctattt caatatgctg agcgattttg
     2101 atttcaccgg cgatatcatt gattcagtta catattccat tcccggaggc ggcaatatca
     2161 ccatatatgc gcagttggat aacgataaat atttcctgga atttgtggca acgccttcag
     2221 tccccggaaa ttactggcga ttgagtacag taggaagcgg ccattttgat atttggtcgg
     2281 ctgaatcaac aacaggtttt tcaaattatg ttaccaccgg tttaccttct gctgttgttt
     2341 taccggagat tgtcaattat cgtttaccgg atacggatca aactattgtg agcagcttcc
     2401 aatgcagcga tcatgtaatc acggtaggca gttatgtgaa tcgcgatagc atgacgaatt
     2461 tttatttgga gaacccgcca tttttcgatg tagtaggtgc cctgttttat tcttcaagtc
     2521 atggccccac acgcgatggt cgcattaaac ctgatatctg cgctcccgga gcacgcgttt
     2581 taagcacggc aagtgataca cttacagaat ggttggcatt gttagatgcc gcaacttatg
     2641 taagtgccga tgctcagcat tatttatata atggcacttc attcagttca ccagctgttg
     2701 ccggaattgc tgcattatat ctgcaaaaaa atccaaacgc caacgcggct gaaataaaag
     2761 atgccattct ttctcaagca agaaaagatg acttcacagg cgctgcattg ccgaataatt
     2821 tatggggata tggcaaagca gatgcattca gaacactcac cggcgattgg ggttgtgctg
     2881 cagatgatta taccgaaccg cctgtcgatt tgcaggtact cgcaaccaca ccaactaaag
     2941 ccatgttaga ttgggatatt ataccaaatg ctgcaggata tcagatttgg tataaatcaa
     3001 gcggtggctc atggaataaa ttgaaatcgc caagccatac aaaaactatt tcgggtttaa
     3061 cgccaaatac tacctacaat gcgaaagtgc gcagttactg cacaggtttt ggattctctg
     3121 atttcagcac cacgattaca tttaccactc caccacttcg tgaagatata tcatccgaaa
     3181 tgctgatcaa tgtgtatccg aatccggcaa gtaatatact gcacgttgat ggcgttgatg
     3241 caggaagcag cattgtgata gtaaatatgc ttggacaagt gatttacaac caaacaacaa
     3301 caaatgccga aaccaatcaa attgatatac acgattgggc tgaaggcatg tatcgcctct
     3361 tgattaacga gcatgacaat tacacattac acacctttat agtagccaga taatcaatgg
     3421 aacaaacact cactcagatc atttctccgg aaaaggtatt g
//
LOCUS       DAOQOU010000251         3268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_21408_length_3268_cov_4.022098, whole genome shotgun sequence.
ACCESSION   DAOQOU010000251 DAOQOU010000000
VERSION     DAOQOU010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21408_length_3268_cov_4.022098"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PLM90_14035"
     CDS             complement(<1..1464)
                     /locus_tag="PLM90_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90552.1"
                     /translation="MSLTAVLPCNMKAQSLPLVVAGGDVKNDSVCDLMVFPGNGPVVL
                     IHGSTNDTTTFYLERWNPDMSLRWSTEFHKSFFEEVPLSMAWNPTTSRLLALGYANPT
                     QNIWGGVVNSIDGTLIGTTELWSDFTFDQEGLCARPVAREVSGFYVFSALDDSFSVYS
                     LDDNGALLGISGVGIPDVFSDPAGALIDIYDDAPYYALTGMYTAIDQPYVSLFWNSDL
                     LYFFDVFTPGICPSAITSNSELNTVVAGNAVDSSAISLAIFDTIGGFHLKMDSTFDSP
                     GYNILCTDIKISNTDKIFVLTKHFTAIDTFHILSLYDTAFNLLSETFVMPGAGDINLS
                     ELDINNIYPDYYAVAGAIKTNNPAYNDQYLIMATSTFGVPGQCVYDCVWPGDADNNGV
                     VDIDDLLPIGAGFGYTGEARVDPSSEWYANLADDWVNTIGTINAKFANTDGNTTINRA
                     DTVYIKSNYLKVHPIYAFRESEDGEIPIWLNTAGITLT"
     gene            1629..3071
                     /locus_tag="PLM90_14040"
     CDS             1629..3071
                     /locus_tag="PLM90_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90553.1"
                     /translation="MANNNNLILKSKFWTQYKTLSDDEAKDFADFVSAGIFARTPKAG
                     RLAKVLRVLRQEPLTVAEVYSRTFEKSAFNEQVLRNYLSELNDALHEFLAMVAMHQRP
                     VLQNWMYLESVRVAAYQPELEKQLEQDRYQPELKTTDDMFAYFRQLQIVDEYAATQAH
                     KKRYDLLVPAMHMLTDHYITAMLRMYCEAVNRKALRSQNYDEEALQFFLTQVAYIEQS
                     RPLTMMAQLFKSVLNIIMFPAEENYFEILKNLLQKHIIDLDKSMAREICLYAQNYCIR
                     HINLNHSTYMQELLSLYDFMILHNVISEGPFMTQYTFKNYITLSLRLHQFDSALHFIK
                     QQQHRLHESVREQAARYNLAAVYFEQGEHGAAMEELFYIQMQDPIYYLDSRAILLKIY
                     FAQQDVEAVGSLYHAVKTYLLRNKQLPRKQAELYRNLFMLSNKLMMLSQNWKYLSPED
                     AMSRKLKLTSKIELSPVANKPWLMTQLNAL"
BASE COUNT         1019 a          674 c          636 g          939 t
ORIGIN      
        1 ggttagtgta atgccggcgg tattaagcca tattggaatt tcaccatcct cgctttctcg
       61 aaatgcataa ataggatgta cttttagata atttgatttg atgtagactg tatcagccct
      121 gttgatagta gtatttccat cggtattcgc gaatttggca tttatcgtac cgattgtatt
      181 tacccaatca tctgcaaggt ttgcatacca ttcagacgac ggatcaacac gcgcttctcc
      241 ggtatatcca aatcctgccc caattggcaa tagatcgtca atatctacaa caccattgtt
      301 gtcggcatcg cccggccata cacagtcgta aacacattgt cccggaacac caaatgtaga
      361 agttgccatt attaaatact ggtcattgta agcgggatta tttgttttaa tagcgcccgc
      421 tactgcataa taatccgggt agatattatt gatgtcgagt tcactaagat ttatatcgcc
      481 tgcacccggc attacaaatg tttcgctgag aagattgaag gctgtatcgt ataatgataa
      541 aatatggaaa gtatcgattg cagtaaaatg tttggtcaga acaaatattt tatccgtatt
      601 gctaatcttg atatcagtac atagtatgtt atatccggga ctatcaaatg tggaatccat
      661 ttttaaatgg aagcccccga tggtatcaaa tattgccaaa gaaattgcgc ttgagtcaac
      721 ggcattacct gcaaccacag tatttagttc cgaattgctg gtgatggctg aagggcaaat
      781 accgggggta aatacatcga agaagtaaag taaatccgaa ttccaaaaca acgaaacata
      841 gggttggtca atagcagtat acatgccggt aagcgcataa tatggtgcat cgtcataaat
      901 gtcgattaac gctcctgccg gatcagaaaa aacatcagga attccgacac ctgaaattcc
      961 taaaagtgcc ccgttatcat ccaatgaata cacgctaaaa gaatcgtcca acgcagaaaa
     1021 cacgtaaaaa cctgaaactt cccgggcaac ggggcgcgcg caaagccctt cctgatcgaa
     1081 agtgaaatca gaccatagtt ctgtagtgcc tatcagcgtg ccgtcaatag aattcaccac
     1141 acctccccaa atattttgcg ttggatttgc ataccctaga gctaataacc ttgaagttgt
     1201 cggattccat gccatgctaa gcggtacctc ttcaaaaaaa cttttatgaa actccgtaga
     1261 ccagcggagc gacatatcgg ggttccatct ttccagatag aatgttgtcg tgtcattggt
     1321 ggaaccatga atcaatacca cagggccatt tccggggaaa accatgagat cgcaaacact
     1381 atcattcttc acatcgcctc cggcaaccac cagagggagt gattgtgcct tcatgttgca
     1441 tggaagcacc gctgtcaatg acattattgc ggtaaataat atggtgcgaa tccttggcat
     1501 ggcgcgtttc tggtgacaaa attgtcttct aaataccctg aaaacaagaa ttatatttta
     1561 taattttaac cactaaatta atatcaatat ttcataaagt attgaatacc aacaaaatat
     1621 agtaaataat ggctaataat aataacctta ttttgaaatc gaaattctgg actcaataca
     1681 aaactttatc agatgatgaa gctaaggatt ttgccgattt cgtttctgca ggaatttttg
     1741 ccagaacgcc taaggccgga cgcctagcga aggtgctgcg cgtcctcagg caagaaccac
     1801 tgacggttgc tgaagtatac agccgcacat ttgaaaaaag tgctttcaac gagcaggttt
     1861 taagaaatta cctatcggag ttgaatgatg cccttcacga atttttggct atggttgcca
     1921 tgcatcaaag gcctgttctc caaaattgga tgtacctgga atcagtgaga gttgctgcct
     1981 atcagccgga actcgagaag caactcgaac aagatcgtta tcagcccgaa ttaaaaacca
     2041 ctgacgatat gtttgcctat ttccggcaat tacaaattgt tgatgaatat gcagcaacac
     2101 aagcacataa aaagcgttac gatttgcttg tgcctgctat gcacatgtta actgatcatt
     2161 atataacagc gatgttacgc atgtactgtg aagcagtgaa tagaaaagca ttgagatcgc
     2221 agaattatga cgaggaagct ttgcaattct ttctcacgca ggttgcctac atcgaacaat
     2281 cgagaccact gaccatgatg gctcagttgt ttaaatccgt gctgaatatt attatgtttc
     2341 ctgccgaaga aaactatttt gaaattttga aaaatttgct gcagaaacat atcattgatt
     2401 tggataagag tatggcaaga gagatttgtc tgtatgcaca aaattattgt atcaggcata
     2461 ttaatctcaa tcacagcact tatatgcagg agttgctatc gctatacgat tttatgatac
     2521 tccacaacgt gatcagtgaa ggtccattta tgacgcaata tacctttaag aattatataa
     2581 cactgtcact gcgcttgcat caatttgact ctgctttgca ttttattaag cagcaacaac
     2641 atcgtttgca cgagtctgtg cgagaacaag cagcgcgata taatcttgca gcagtatatt
     2701 ttgaacaggg tgagcatggc gctgctatgg aggagttatt ttatattcaa atgcaagatc
     2761 ccatttatta tttggactct cgtgccattt tattgaaaat ttacttcgca caacaagatg
     2821 tcgaggcagt aggatcctta tatcacgcag taaaaacata cctgcttagg aataaacagc
     2881 ttccacgtaa acaagctgaa ttgtatcgca acctattcat gttgtcaaac aagctgatga
     2941 tgctttcaca aaattggaag tatctatctc cggaggatgc tatgtcgcgt aaattaaaac
     3001 ttacttcaaa aattgaatta agtcctgttg ccaataaacc ctggcttatg acacagctta
     3061 atgcactttg atttgcctga cgtccacaaa cttatatatg gctctgaagg gaagtggctt
     3121 tgttttgcct ttgaacacca ataaatgcct tcagatatgc gtaacgaatt aattattact
     3181 tcggctatat acctctaaat tttacatcag ttcaattcga cctcaaatat tcatcggcat
     3241 ccttatcaaa aaagaaactt acatccat
//
LOCUS       DAOQOU010000252         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_21530_length_3257_cov_4.827920, whole genome shotgun sequence.
ACCESSION   DAOQOU010000252 DAOQOU010000000
VERSION     DAOQOU010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21530_length_3257_cov_4.827920"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLM90_14045"
     CDS             <1..284
                     /locus_tag="PLM90_14045"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HMZ90554.1"
                     /translation="LIPVVIYSMVITAMLMQSLRRWSKTDNYSFWFVAIGATFFIASD
                     SMIAIDSFYSPINFAPLLIMITYIIAQYMLVTGLLLHKDKVINQPQSDQ"
     gene            complement(259..1278)
                     /locus_tag="PLM90_14050"
     CDS             complement(259..1278)
                     /locus_tag="PLM90_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMZ90555.1"
                     /translation="MDEQRMNIRLQKIVVAIAIILLLVKFSAYLLTHSNAILTDALES
                     IVNVVAGAFGLYSLILASKPRDINHPYGHGKIEFISASIEGSMITLAGVLIIGKSIFN
                     LLYPQTIQNVESGIILVAIAGTVNFAMGIFMERRGKKANSLILIAGGKHLQSDGWSTA
                     GLLVGLGLLLIFKKPCIDSAVALLFGAYIAYEGYQILRKSVAGIMDEADTELLRSLID
                     TFEKHREDDWIDIHNLRVIKYGANLHFDCHLTVPYYYSVKEGHDALERMESLIHVHHE
                     TSDEWFVHIDACVPPAMCKICTKRDCQHRQSPLESRIEWNLENVLRNQKHGLYGPTID
                     PTEVD"
     gene            complement(1450..>3257)
                     /gene="polA"
                     /locus_tag="PLM90_14055"
     CDS             complement(1450..>3257)
                     /gene="polA"
                     /locus_tag="PLM90_14055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMZ90556.1"
                     /translation="ISNVQHTYILADTPEKRKHLVQQLEKQSIFCFDTETTGIDANVA
                     ELVGLAFSWQQHQGYYVPVPADKKEADKIIAEFKPVLESAKHTKVAQNLKYDYLALKW
                     HGVDVVGPFEDSMIAHYLLEPELRHNMDYLAENYLGYTPVSIETLIGKKGKNQLSMRD
                     APIDKVAEYSAEDADITLQLHQVFAPMLKKEELDTLYRTVEMPLIPVLADMEYEGISL
                     DVDFLKEYSTLLEKDMLELEKKIYEHAGVRFNIDSPKQLGDVLFIKMKIPYEGQKTKT
                     GQYSTGEEVLAKLAHAHPLAGLILDYRELGKLKSTYVDSLPLLINPKTHRLHTTFAQA
                     VASSGRLSSNNPNLQNIPIRTERGQKVRRAFIPRDKHHKILSADYSQIELRIIAALSN
                     DPAMIDAFKNGMDIHSATAARVFGVPINEVTREMRSKAKAVNFGIAYGQTAFGLSQNL
                     NISRGEAKEIIDNYNAQFPGVKQLMESNIEFARKHGYTQTILGRKRYIKDINSANNTV
                     RSGAERVAVNSPIQGSAADLIKVAMLHIHEALNTKKMRSRMLLQVHDELVFDAHVDEL
                     DELKALVVEKMVNAIKMNVPIEAEVGIGDNWLEAH"
BASE COUNT          875 a          702 c          671 g         1009 t
ORIGIN      
        1 tgttgattcc cgtagtaatt tattcaatgg ttattaccgc aatgctgatg caatcgttgc
       61 gcaggtggtc gaagactgat aattatagtt tttggtttgt tgcaattggc gctacgtttt
      121 ttattgcctc tgattcaatg atagccattg attcatttta ttcacccata aatttcgcac
      181 cactgctgat catgattaca tatatcatag cacaatatat gcttgtaacg ggattgctgt
      241 tgcataaaga caaggtgatc aatcaacctc agtcggatca atagttggtc cgtagagacc
      301 atgtttttga ttgcgaagta cgttttccag attccattca atgcgtgatt ccaagggaga
      361 ttggcgatgc tgacaatctc gcttcgtgca aatcttgcac atggcaggtg gaacacaggc
      421 gtcgatatgt acaaaccatt catcagaagt ttcgtggtgc acatgtatca gtgactccat
      481 gcgttccagt gcatcgtgcc cttctttaac tgaataataa tatggaacag taagatgaca
      541 atcaaagtgc agatttgcac catatttaat tacgcgaaga ttatgaatat ctatccagtc
      601 gtcttcacga tgtttttcaa atgtatcgat gagagaacgc aataactcgg tatccgcttc
      661 atccatgatg ccggcaacac ttttgcgcaa tatttgatat ccttcgtagg caatatatgc
      721 accgaataat aatgccacgg cactatctat acatggtttt ttgaaaatca gcaacaagcc
      781 gagtccaacc aataaaccgg ccgtactcca gccgtcactt tgcaaatgtt tacctccggc
      841 gatgaggatg agtgagtttg cttttttacc tcggcgctcc atgaaaatcc ccatggcaaa
      901 atttacagtt cctgcaattg caaccagaat aatacctgat tcaacattct gaatggtttg
      961 cggatacaat aaattaaaaa tggactttcc gataatcagc actcccgcca aggttatcat
     1021 actaccttca atgctggcgg aaataaattc aatttttccg tgaccataag gatgattgat
     1081 gtcgcggggt tttgaggcaa ggatcaaact atataatcca aaagcacctg caacaacatt
     1141 tacaatgctt tcaagtgcgt cggtaagaat tgcgtttgaa tgtgtaagga gataagcact
     1201 gaattttacc aaaagcaaaa taatggctat tgcaaccaca attttctgca accgaatgtt
     1261 catccgctgc tcatccatca tgcaaaggta ttgaaatatt aattcaagta gtgggtagtg
     1321 agtagtgaga ctagctcaat actgactgta acttcgttac agtaaatgca ttaaaactct
     1381 gcgtctttgc gcctctgcgt gaaaaaaaga ctcacgattc acgcttcacg actcacgata
     1441 ctcacccact caatgcgctt ccagccaatt atcgccaatg cccacttcgg cttcaatggg
     1501 gacattcatt ttgatagcgt ttaccatttt ttcaactacc aatgctttca attcatcgag
     1561 ctcatcaaca tgcgcgtcga agaccaattc atcatgtacc tgcagcagca tgcgcgaacg
     1621 catttttttc gtgttgaggg cttcgtgaat atgtagcatg gctactttaa tcaggtcggc
     1681 ggcagagcct tggataggag agtttacggc aacacgttct gctccactgc ggactgtgtt
     1741 gttggcagaa ttgatatcct tgatatagcg ctttcggccc aaaatagttt gcgtgtaacc
     1801 atgcttgcgg gcaaattcaa tattgctttc catcaattgc ttcacacccg ggaactgcgc
     1861 attgtagttg tcgataatct ctttggcttc tccgcgagaa atatttaaat tttgtgagag
     1921 tccgaatgct gtttgtccgt atgcgatacc aaaattcact gccttggctt tactgcgcat
     1981 ttcacgtgtc acttcgttga taggaacacc aaatactcgc gcggcagttg cactgtgaat
     2041 gtccatgcca tttttaaaag catcgatcat ggcaggatca ttactgagtg ctgcgatgat
     2101 gcgcaactct atttgagaat aatcggcgct gagtattttg tgatgtttat cgcgtggaat
     2161 aaatgctctg cgcacttttt gtccgcgttc ggtgcgaatg ggaatatttt gcaaattcgg
     2221 attattggat gataatcgtc cgcttgatgc cacggcttgc gcgaatgtgg tatgcaggcg
     2281 atgtgtcttt ggatttatca ataatggcaa tgaatccacg tatgtagatt tcaatttacc
     2341 taattcacga tagtcgagaa tgagtcctgc aagcgggtgc gcatgtgcca gtttcgcaag
     2401 cacttcttcg ccggtgctgt attgtcctgt ttttgttttt tgtccctcgt aaggaatttt
     2461 cattttaatg aacaacacgt caccaagttg ctttggagag tcaatattaa agcgcacacc
     2521 tgcatgctca tagatttttt tctccagttc gagcatgtct ttttcaagca gggtgctgta
     2581 ttctttcagg aagtcgacat ccagactgat accttcatat tccatatcgg ctaataccgg
     2641 aatgagcggc atttccacgg tccggtatag tgtatccagt tcttcttttt tcagcatagg
     2701 cgcaaacacc tgatgcagct gcaaagtaat atcagcatct tcggctgaat attcagctac
     2761 tttatcaatt ggtgcatcgc gcatgctcag ttgattcttg ccttttttac cgatgagtgt
     2821 ttcaatgctc accggtgtat atcccaggta attctcggcg agataatcca tattatgtcg
     2881 caactcaggt tccagcaaat aatgtgcgat catactatct tcaaacggac caacaacatc
     2941 aacaccatgc cattttaaag cgaggtaatc atatttcaaa ttttgtgcaa cttttgtatg
     3001 ttttgcactt tctaaaacag gtttaaattc tgcaatgatc ttatcagctt cttttttgtc
     3061 ggcaggaaca ggcacataat atccttgatg ttgctgccat gaaaatgcaa gcccgactaa
     3121 ttcggcaaca tttgcatcaa taccggtagt ttcagtgtcg aagcaaaaaa tagattgttt
     3181 ctccagctgc tgaacgagat gcttacgttt ttctggtgta tcagccagaa tgtaggtgtg
     3241 ctgtacattt gagatat
//
LOCUS       DAOQOU010000253         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_21710_length_3241_cov_6.632768, whole genome shotgun sequence.
ACCESSION   DAOQOU010000253 DAOQOU010000000
VERSION     DAOQOU010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21710_length_3241_cov_6.632768"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..857
                     /locus_tag="PLM90_14060"
     CDS             57..857
                     /locus_tag="PLM90_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ90557.1"
                     /translation="MQENLTNVPIMAELFANGESPEVLFWVGCAGSFDDRAKKITGAF
                     VRILNSIGVRFAVLGTEESCTGDPARRAGNEFLFQMLAQNNIAVMNGYGIKKIVTTCP
                     HCFNILKNEYPALGGEYEVLHHTAFLQQMIDEGRLKVEGGAFAGKRITYHDSCYLGRA
                     NDIYEAPRNVLIQLDAQLAEMKSCKSRGLCCGAGGAQMWKEAEHGNKEINIERTEQAL
                     EVNPDIIASACPFCNTMMTDGVKMKEKEEGVKVFDVAELVAMANGLFA"
     gene            854..1360
                     /locus_tag="PLM90_14065"
     CDS             854..1360
                     /locus_tag="PLM90_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90558.1"
                     /translation="MIATENNSIEKLIADFDPASKVWVYQSSRPFTSAEENEISQALQ
                     SFTKQWAAHNIQLKATGFIVESRVIVLMVDETHTDASGCSIDTSVHFIQGLENEYGVE
                     LFDRMIVHYRDVEGWHTTTLSTLPDLISQHLISKSTIVINPLVATKEEYQHSFVTRIE
                     DCWMMQFV"
     gene            1404..2690
                     /locus_tag="PLM90_14070"
     CDS             1404..2690
                     /locus_tag="PLM90_14070"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HMZ90559.1"
                     /translation="MKTLLTLVFCTFLSLASFAQATDSTKTDSINSAYLNLYLDGGYG
                     YQDYIKTEIPFVNYVRDRFDGDVHLLITAQGTGSGGADYTLFFLGQKTFEGKNDTLHY
                     IANTNATSEEIRAGLVQVIKMGLLPFIAKMNAAFPVQITFTGSTNSEERQENDKWKGW
                     VYSISSYANASLTSNYESYSISGNVSADKVTEEWKLGFSYGANYNTDVFDFGDGDIYE
                     SFNNSTYGNATIVNSLNDHWSLGGEGFYSTSTYSNYKTNIAIAPGIEYDVFPYDESTT
                     KLLTFFYKVGPEYHHYIDSTIYNKTEELLMRQRLDVSLSLTQKWGSITVGINGSNYFH
                     DLTKNGAGCFTSINWRIVEGLSLNGFFTLDFINDQLNIPKGDLTDEEILLQITERQTN
                     YSFYSFFGLSYTFGSIYNNVVNPRFSGGSYSFYFSN"
     gene            2737..3240
                     /locus_tag="PLM90_14075"
     CDS             2737..3240
                     /locus_tag="PLM90_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90560.1"
                     /translation="MYTPSFLYIHKQRSMHDNYYVFDTIMGAKYPFWYMVILFIFQMG
                     ALVKDNWVLTVAFLPMFIIFAMYEGVEVNIAKMQYRDYTHLFQWWWGRFKPIEDPDYL
                     LLSRTSDTRNGGIRYDVHLITKGSRDLLLFRTKNLEDHENVVKVLAQRTGWKAYRNTI
                     DGLEGFE"
BASE COUNT          982 a          583 c          724 g          952 t
ORIGIN      
        1 actggaaaaa tgaaatttaa tctgtaagca agctttacac agtaagctaa atgaatatgc
       61 aggagaatct gactaatgtg ccgataatgg ccgaattatt tgcaaatggc gaatcaccgg
      121 aagtgctgtt ttgggttgga tgtgccggta gtttcgatga tagagcgaaa aaaataaccg
      181 gagcatttgt aagaatttta aacagcatag gcgttcgttt tgccgtgcta ggaacagaag
      241 agtcatgcac cggagatcct gcccgccgag caggtaatga atttttgttt cagatgctgg
      301 cgcaaaacaa tattgcggtt atgaatggct atggtattaa aaaaattgtt accacctgtc
      361 cgcattgctt caatatcctt aaaaatgaat accctgcgct tggtggtgaa tatgaagttc
      421 ttcatcatac ggcattcctg caacaaatga tagatgaagg acgcctgaaa gtggaaggag
      481 gtgcatttgc aggtaagcga attacttacc atgattcatg ttatctcgga agagcaaatg
      541 atatttacga agcgcccaga aatgtattaa ttcaactcga cgcgcaattg gcagaaatga
      601 aaagctgtaa atcgcgaggt ctttgctgtg gtgccggtgg tgcgcaaatg tggaaggaag
      661 cggaacatgg caataaagaa ataaatatcg aacgtacgga gcaggctttg gaagtaaatc
      721 cggatatcat tgcatctgct tgccctttct gcaataccat gatgacagat ggcgttaaaa
      781 tgaaagaaaa agaagaaggg gtcaaagttt ttgatgtagc cgaattagta gctatggcaa
      841 acggattatt cgcatgatag caacagaaaa caatagcata gaaaagctta tcgctgattt
      901 tgatcctgct tcaaaagtat gggtatatca atcttcaaga cccttcacat cggctgagga
      961 gaatgaaatc tcacaggcct tgcaatcctt caccaaacag tgggcggcgc ataatattca
     1021 attgaaagca acaggtttta tcgttgaatc acgcgtgatc gttttaatgg ttgatgaaac
     1081 gcataccgac gccagcggat gttctattga cacctcggtg cactttattc aaggtttgga
     1141 aaatgagtat ggtgttgaat tgtttgatag aatgatagtg cattatcgcg atgtggaggg
     1201 atggcatact accacattat ctaccttgcc cgatttaatt tcgcagcatc ttataagtaa
     1261 atcaacaatc gtaataaatc cattggtagc aacaaaagag gaatatcaac actcatttgt
     1321 tacaaggatt gaagattgct ggatgatgca attcgtataa tatttagcat acttagtctt
     1381 acactctcta cgttattcat attatgaaaa ccctcctcac gcttgtattt tgtacattcc
     1441 tgtcccttgc atcgtttgca caggcaacag attccacgaa aaccgattct attaattcgg
     1501 catacttgaa tttatatctg gatggcggat acggctatca ggattatatc aaaacagaaa
     1561 taccttttgt aaactatgtg cgcgacagat ttgatggtga tgtgcatttg ttgattaccg
     1621 cccaaggtac cggaagtggt ggtgccgatt atactttgtt tttccttgga cagaaaacat
     1681 ttgaaggaaa aaacgatacg ctgcattata tcgcgaatac caatgccacc agcgaagaaa
     1741 ttcgtgccgg attagtgcag gttattaaga tggggttgtt gccatttatt gcaaaaatga
     1801 atgccgcctt tccggtgcaa attacattca caggcagtac aaacagcgaa gagcgacaag
     1861 aaaatgataa gtggaaggga tgggtgtatt ctatatcgtc atacgctaat gccagcctta
     1921 cctccaacta tgaatcttat tcaatttcag gaaatgtgtc ggccgacaag gtgacggagg
     1981 aatggaaact cggattctca tacggagcga attataatac cgatgtgttt gattttggtg
     2041 atggcgacat ttatgaaagt tttaataata gcacctatgg aaacgcaacc attgttaata
     2101 gtttaaatga tcattggagt ttaggtgggg aagggtttta tagcacgagc acctacagca
     2161 attataaaac aaatattgcc attgctcccg gaatagaata tgatgttttt ccttatgacg
     2221 agtctaccac caagttgcta accttttttt acaaagtggg acctgagtat catcattata
     2281 tcgactcaac aatttacaat aagactgaag aattattaat gcgccagcga ttggatgtgt
     2341 ctttgtcgct cacacaaaag tggggttcta ttactgtcgg catcaatggc tctaattatt
     2401 tccacgacct caccaagaat ggcgcaggct gttttacatc tataaactgg aggatagtgg
     2461 aaggcttgtc gttaaacgga tttttcacac ttgatttcat caatgatcaa ctgaacatcc
     2521 ctaaaggcga tctcacggat gaggaaatct tattgcaaat aaccgagcgt caaacaaatt
     2581 atagcttcta ttccttcttt gggctatcat atacttttgg ctccatttac aacaatgttg
     2641 taaacccgcg ttttagcgga ggctcgtata gtttttattt cagtaattga tgaggatgct
     2701 tatattggat attgttaaag cgtaattgaa tttgttatgt atacaccaag tttcttgtat
     2761 atacataagc agcgttcgat gcatgataac tattatgttt ttgacaccat tatgggcgcc
     2821 aagtatccct tttggtatat ggtgatatta tttattttcc aaatgggcgc ccttgtgaag
     2881 gataattggg tgctgacggt agcatttctt cccatgttta tcatttttgc catgtatgaa
     2941 ggtgttgaag taaatattgc gaagatgcag tatcgtgatt acacgcattt gtttcaatgg
     3001 tggtggggga ggtttaagcc aattgaagac cctgattact tactcctgtc gagaacatca
     3061 gacacccgca atggcggcat tcgctatgat gtccacttga ttacaaaagg cagtcgcgat
     3121 ttactattat ttcgcaccaa aaaccttgag gaccatgaaa acgttgtcaa agtattagcg
     3181 cagcgcaccg gttggaaggc atataggaat actattgatg ggttggaggg gtttgaatga
     3241 g
//
LOCUS       DAOQOU010000254         3236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_21760_length_3236_cov_5.728073, whole genome shotgun sequence.
ACCESSION   DAOQOU010000254 DAOQOU010000000
VERSION     DAOQOU010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21760_length_3236_cov_5.728073"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..480
                     /locus_tag="PLM90_14080"
     CDS             235..480
                     /locus_tag="PLM90_14080"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HMZ90561.1"
                     /translation="MDNFLYFILLGAIAGYVSGKLIKGESFGLLGNIVVGVVGAVLGG
                     WIFDQVGIQEANILYSLAAAVVGSVAFLLIIGLLKRK"
     gene            complement(481..900)
                     /locus_tag="PLM90_14085"
     CDS             complement(481..900)
                     /locus_tag="PLM90_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ90562.1"
                     /translation="MKRVTGIGGIFFRAKGEVKELNSWYEKHLGFDYINSNGYPFWAW
                     RSMEDPEKIGASIWSIFPRDTKYFGSNDQQFMFNFRVENMVELIETLRSEGVEIAGEI
                     QEFEYGKFCWIIDPDGNKIELWEAPENPGFEGGMPAK"
     gene            complement(934..>3236)
                     /locus_tag="PLM90_14090"
     CDS             complement(934..>3236)
                     /locus_tag="PLM90_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ90563.1"
                     /translation="MRSLFIKFQLFCLLQCIMIAGNAQSITGNITDATTREILPGVNI
                     TIADHGGVSSDENGNYNLQLPPGKYVLNFSFLGYETKQITVVLGKDDIRQLPVILEPA
                     SQLLDPIVVSGSLFEKKQSEETISMVIADQRLLEHTNTTNLSDAITKVPGVYMMDEQA
                     NIRGGTGFTYGAGSRVMLVVDDQILLAADRGDAKWNFVPMENVEQIEIIKGASSVLYG
                     SSALNGVIAVRTAWPGLTPETNISTYHTTFDKPALADAAWWDHAPTQSGISVSHRQKF
                     ENMDLVIGGNVASHDSYLLGQYSSRARLNWKTRFHPDAHDQLTWGVNGNLMSDREGLF
                     FLWQDADTGGFQPFQGYLDTTTTTLLNWQYKWATIDPWLNYYDKHHNSHKFKMRFYGS
                     DVIYTDTTDGNAWLLNADYQFFREFASDVVLTAGATGYYFDVDDHDVGIHDGISAGAY
                     VQLEKKLFDRLIINAGMREELYQVDTAEGASVPIFKGGLNYRLGASTNIRASFGQGYR
                     FPSLVEKYARSNLGTLFIFPNPDLKPEYGWNTEIGFIQAINRDAWRSTVDVAFYLTEY
                     FDMTEFTFNFYSGEGAGFKSLNTSRARIGGMEIIFKGDMELFGNRLTWIGGYNYIYPA
                     DISGDSSNLEFGHFMQNFIDGFTASSGDSVFLESVLKYRFRHTGRLDLDYETDKWLFG
                     ADVNYYSLMENLDAIYISFIPGIVEYREDHMHGDWIADARVGYKINKKMQVQLLGKNI
                     FNNMYALRPAKYDPPRSFTVQWRVEL"
BASE COUNT          929 a          762 c          562 g          983 t
ORIGIN      
        1 aaatattgca aagcaatata attgcattcc ctattaacgc gccagcgtta atagcaccac
       61 cacttgctaa tttgctaatt ctcaaacctg ctaactaaga agataaaccc gatgcaagca
      121 atcaccccca tttgctaatc tgctaattct caaatttgct aattaactcc ccaaattcct
      181 ctctcctttc tcctttctcc tctttcctct ctcctattaa ttatctttgc ctccatggac
      241 aactttcttt atttcattct tcttggcgcg attgccgggt atgtgagtgg taagctaatc
      301 aaaggtgaaa gctttggatt gctgggaaat attgtagttg gtgttgttgg cgctgtgtta
      361 ggaggatgga tcttcgatca ggtaggtata caggaagcca atatccttta cagtctggca
      421 gcagctgtag taggatcagt agcatttctg ctgatcatcg gattactgaa gcgcaaataa
      481 ttatttagca ggcattccac cttcgaagcc aggattttcg ggagcttccc acaattctat
      541 tttattgcca tcagggtcta tgatccagca gaatttacca tattcgaatt cctgaatttc
      601 acctgctatt tctacgcctt cgctcctcaa tgtttcgatg agttcaacca tattttctac
      661 acgaaaattg aacatgaact gctgatcatt gctaccaaag tactttgtat cacgaggaaa
      721 gatgctccag atggaagcgc caattttttc aggatcttcc atgctgcgcc acgcccagaa
      781 tggatatcca ttagaattga tataatcgaa acccaaatgt ttttcatacc agctatttaa
      841 ttctttcact tcgccctttg cgcgaaaaaa aataccgcct atgcctgtta ctcttttcat
      901 gatatcagat ttatgatgtt gaatatactg aaattacaat tccacccgcc attgcacagt
      961 gaaacttctt ggcggatcat attttgcagg tcgcaaggca tacatattat taaaaatatt
     1021 tttgcccaat aattgcacct gcattttctt gtttatttta taccctactc gtgcatctgc
     1081 tatccaatcg ccatgcatat gatcttcgcg atattctaca atgcccggaa taaatgaaat
     1141 atagatggca tccagattct ccatcaggct gtaatagttt acatccgcac caaacaacca
     1201 tttgtctgtt tcgtaatcca aatccaatcg accggtatga cggaagcgat attttaatac
     1261 agattccaga aaaacggaat ctccggaaga agctgtaaat ccgtcaataa agttctgcat
     1321 gaaatgtccg aattcgaggt tggaagaatc tccggaaata tctgcaggat agatataatt
     1381 atatccgcct atccacgtca acctatttcc aaataattcc atgtcgcctt taaaaattat
     1441 ttccatgcca ccgattcgtg cacgtgaagt attcaaggat ttgaatcccg cgccttcacc
     1501 cgaataaaaa ttaaaggtga attcagtcat gtcgaaatat tctgtgagat aaaaggctac
     1561 atcaacagta cttcgccaag catctctgtt gattgcctga ataaaaccga tttctgtatt
     1621 ccaaccatat tccggtttta aatccgggtt aggaaaaata aacaaggtgc cgaggttaga
     1681 ccgcgcatat ttttcaacca aactcggaaa gcgatatcct tgtccgaagg aagccctgat
     1741 atttgtagat gcacccaatc gataatttaa tccacccttg aaaataggta cagatgcccc
     1801 ttcagcagta tccacctgat acagttcttc acgcattccg gcgttgatga ttaatctgtc
     1861 gaaaagtttc ttttctaatt gcacataagc acctgcactg atgccgtcat gaatgccaac
     1921 atcatgatca tccacatcaa aataatatcc tgttgctccg gcagttaata ccacatctga
     1981 agcaaattct ctgaaaaatt gatagtcggc attcagcaac catgcattgc catcggtagt
     2041 gtcggtgtag ataacatcag aaccataaaa gcgcatttta aacttatgac tgttatgatg
     2101 tttatcataa taattcagcc acggatcgat agtagcccat ttatattgcc agttgagcaa
     2161 tgtggtagta gtggtatcca aatatccctg aaagggttga aatcctcccg tatctgcatc
     2221 ctgccacaaa aaaaagaggc cttccctatc cgacatcaga tttccattca cgccccaggt
     2281 caattggtca tgtgcatcag gatgaaatcg cgttttccaa tttaatcgcg cacgagagga
     2341 atattgtcct aataaatatg aatcatgcga tgctacattt ccacctatca ccaaatccat
     2401 attttcgaac ttctgtcgat ggctaacact tataccactt tgtgtaggcg catgatccca
     2461 ccatgcagca tctgccagtg ccggtttatc gaaagtggta tgataagtgc tgatatttgt
     2521 ttccggtgtc aaccccggcc aagccgtgcg cacagcaatt acgccattta atgcactgct
     2581 gccatataat accgatgaag cccctttgat gatttctatc tgctccacat tctccattgg
     2641 aacaaaattc cattttgcat caccgcgatc tgctgccagc aatatctgat catcaacaac
     2701 caacataact ctgcttccgg cgccataggt gaatcctgtt cccccgcgaa tattggcctg
     2761 ttcatccatc atatacacac ccggcacttt tgtgatcgca tcacttaaat tagtagtgtt
     2821 ggtatgttca agcaatcgtt gatcggcaat aaccatgctg attgtttctt cgctttgttt
     2881 cttttcaaat aaactaccac tcacaactat cgggtccaat aattgcgaag ccggttctaa
     2941 aattacgggt aattgcctga tatcgtcctt tcctaaaact actgttattt gcttcgtttc
     3001 gtatcccaga aaagaaaaat tcagcacata ttttccggga ggcaattgca agttataatt
     3061 tccattttca tcagaactta caccaccatg atccgcaatg gtaatattta ctccaggcaa
     3121 tatttctctt gtggtagcat ctgtaatatt tcccgtaata gactgcgcgt ttcccgcaat
     3181 catgatgcat tgcaacaaac agaacagttg gaattttatg aataggctac gcataa
//
LOCUS       DAOQOU010000255         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_21837_length_3230_cov_6.302362, whole genome shotgun sequence.
ACCESSION   DAOQOU010000255 DAOQOU010000000
VERSION     DAOQOU010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21837_length_3230_cov_6.302362"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLM90_14095"
     CDS             complement(<1..584)
                     /locus_tag="PLM90_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90564.1"
                     /translation="MRSIAIILMAAFSASPLMAQKDSGHNKLELHVGVVTSFTMPLGA
                     SDYDKSLFTTFIPAINYRGQVIYAHQLGLESSLHTGMGLGVSHYSFKLTMPSTGNSNR
                     SFKGSVACLDAQLPLYTKFTIAKGKHNSNYAMTAGAELHAVNIFSQGFTATSEWEETI
                     FEVSESDHIQLMATARLGIEGNHIGNNTFFGWCLL"
     gene            796..2220
                     /locus_tag="PLM90_14100"
     CDS             796..2220
                     /locus_tag="PLM90_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ90565.1"
                     /translation="MILEQIYTGCLAQGAYYLESNGEAAIFDPLREVQPYIDMANKRS
                     ATIKYVFETHFHADFVSGHADLAKKTGATIVYGPTKMETGFTAHIGTDGEIFKIGDIT
                     IKLLHTPGHTMESSCFLVHDEQGKPTAIFTGDTLFIGDVGRPDLAQHVIADLTQENLA
                     AHLYASLQEKILPLPDDLIVYPAHGAGSACGKNLSKETSDTLGNQKKFNYALRPGLTK
                     EQFISELLSGLTTPPSYFPKNVLMNIQGYDTIDTVLERGKKALSPDAFELIANETGAL
                     IIDTRKAADFASGFIPNSINIGHNGTFAVWVGTLVPDIKQEILIVAEKGREEEAITRL
                     ARVGYDHAIGFLDGGFDAWKNAGKEIDTIKTISVSEFIEAKNADNKIAILDVRKKSEF
                     DSEHIVEAENMPLDYINDYFTAIDKQKTYYVHCAGGYRSMIFASILRARGYNNLIDVD
                     GGFNAIKASGKFNITDYVCPTTML"
     gene            2222..2431
                     /locus_tag="PLM90_14105"
     CDS             2222..2431
                     /locus_tag="PLM90_14105"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HMZ90566.1"
                     /translation="MKKLKPYLSTIFGIAVGMLAGYVWYDYNACSTGTCPITSHPLNA
                     IVYGGVMGGLFFSSFKKKQKTNTIK"
     gene            2428..2679
                     /locus_tag="PLM90_14110"
     CDS             2428..2679
                     /locus_tag="PLM90_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ90567.1"
                     /translation="MSKQTTIIDVRTPAEFIGGHVAGSINIPLNEIPAKVDEIKAMEQ
                     PIILCCASGNRSGQATMYLQQFGIDCSNGGSWLMVNALV"
     gene            complement(2742..>3230)
                     /locus_tag="PLM90_14115"
     CDS             complement(2742..>3230)
                     /locus_tag="PLM90_14115"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMZ90568.1"
                     /translation="AISFLPNLIPLFITGAIMGFMHIAIKPSTVLVFSIAYGMCVDFT
                     CYFIAKYKHDLIRNSFNIPKTVMSSLQEAGVSMIYTSLILFCGFFIFVFSKFGGTVNM
                     GLLTSITLLVALFNNLLLLPALMLLFEKGLNKSNRKFIEKSSHEKASRPFTDLIDEHQ
                     KN"
BASE COUNT         1002 a          621 c          704 g          903 t
ORIGIN      
        1 agtagacacc agccgaagaa agtattattt ccgatatgat ttccctcaat tcctaagcgg
       61 gctgttgcca tcaattgtat gtgatcgctt tccgaaactt cgaatatcgt ttcttcccat
      121 tccgatgttg ccgtgaatcc ctgactaaaa atattcactg catgtaattc ggcaccggct
      181 gtcatggcat aattgctgtt atgctttcct ttagcgatgg tgaacttagt atacaatggc
      241 aattgtgcat caagacacgc cactgatcct ttaaaggatc tattgctgtt accggtagat
      301 ggcattgtta gcttgaaact ataatgtgac accccgagcc ccattccggt atgtaacgaa
      361 gattccagcc ccagctggtg ggcataaata acttgtccgc gatagttgat cgcaggaatg
      421 aaagtggtaa ataatgactt gtcgtaatca gatgcgccca agggcattgt aaagctcgtt
      481 actactccga cgtgcaattc caacttgttg tgtccggaat ctttttgggc cattaagggg
      541 cttgcgctga atgcagccat cagaataatt gcaattgatc tcatggggta gatgttgttg
      601 acagataggt aacgcttaat tcaaaatttg cgtatggcaa gcttctgaaa agtattgatg
      661 cttttgtaac aaaagtaact gtgtaacaaa agtaacgata tggcaatgct tggtgcgaca
      721 tctttgcatc ctatcaggag agagccgaca actgcatcga ccttgtatat ctattaacat
      781 gataaaatac aaactatgat actggaacag atctacacag gatgcctggc acaaggcgca
      841 tattatctgg agagcaatgg tgaagctgca attttcgatc cgctgaggga agtgcagcca
      901 tatatcgaca tggctaacaa acgcagtgct accattaaat atgtttttga aacacatttt
      961 catgccgatt tcgtttcggg acatgccgat ctggctaaaa aaacaggtgc gacaattgtt
     1021 tatggaccca ctaaaatgga aaccgggttt accgcacata ttggcacaga tggtgaaata
     1081 tttaaaatcg gagatatcac tatcaagttg ctccacactc ccggacacac catggaaagc
     1141 agctgctttt tagtgcacga tgaacaagga aaacccacag caatatttac cggcgacacc
     1201 ttgtttattg gcgatgtagg gcgtccggat ctcgcacaac atgtaattgc agatttgaca
     1261 caggagaacc ttgccgcaca tttgtatgct tctctacaag agaagatatt accacttccc
     1321 gacgatctaa ttgtttatcc ggcacatgga gcgggaagtg catgcggaaa aaatctcagc
     1381 aaggaaacat ctgacacctt gggtaatcag aaaaaattca attatgcctt gcgtcccgga
     1441 cttaccaaag aacagtttat cagcgaattg ttatcaggtc ttacaacgcc tccgtcttat
     1501 ttccccaaaa atgtattaat gaatattcag ggatatgata caatagatac tgtattggag
     1561 cgtggaaaaa aagcactctc accagatgca tttgaattaa ttgcgaatga aaccggtgcc
     1621 ctgattattg atacaagaaa agcagcggat tttgcaagtg gatttattcc gaattctatc
     1681 aatattggtc ataacggcac attcgcagta tgggttggga cattggtgcc ggatataaag
     1741 caagaaattt tgatcgttgc tgaaaagggc agagaagagg aggctattac ccgtctggca
     1801 agagtaggtt atgatcacgc tatcggattt cttgacggcg gatttgatgc atggaagaat
     1861 gccggaaagg aaatagatac tattaaaaca atttcagtct cagaatttat cgaagcaaag
     1921 aatgcagata ataaaattgc cattcttgat gtccgcaaaa aaagtgaatt tgatagcgag
     1981 cacattgttg aggcggaaaa tatgccgttg gattatatca atgattattt cacagctatc
     2041 gataaacaaa aaacatatta cgtgcattgt gcaggtggat atcgttcaat gatttttgcc
     2101 tctatcttga gggcaagagg ctacaataat ttaattgatg ttgatggtgg atttaatgcc
     2161 atcaaagcat caggtaaatt caatataact gattacgttt gtccgacaac tatgttgtaa
     2221 tatgaagaag ctaaaaccat atctatctac tatttttggc atcgcagttg gtatgcttgc
     2281 ggggtatgta tggtatgatt ataatgcttg cagcacaggc acttgcccca tcacatcgca
     2341 ccctttgaat gcaatagttt atggcggcgt tatgggggga ttattttttt catcattcaa
     2401 aaagaaacaa aaaacaaata cgataaaatg agtaaacaga caacaattat cgatgtacgc
     2461 acacctgcag aatttatagg tggtcacgtg gcggggagta tcaatattcc attaaatgaa
     2521 attcctgcta aagtagatga gataaaggca atggaacaac ccatcattct ctgttgcgcc
     2581 agcggtaaca gaagtggtca ggcgacaatg tatttacagc aatttggcat cgactgctcc
     2641 aatggcggaa gctggttgat ggtaaatgcg ctagtttaat taatgacaat cttattgaag
     2701 tccggtggcg ccgctatcgg acttttttat tttcgtaaag atcagttttt ctgatgttca
     2761 tcgatcaaat ctgtaaacgg acgggaagcc ttttcgtgac tcgatttttc gatgaatttg
     2821 cggtttgatt tgttcaagcc tttttcaaac aacaacatta aagcggggag caataataaa
     2881 ttattgaata gcgccaccag cagtgtgata gaggttaaca gacccatatt cacggtgccg
     2941 ccgaatttgc tgaaaacgaa aataaagaaa ccgcagaaca gaatcaacga agtgtatatc
     3001 atactcaccc cggcttcctg caaactgctc attacggttt tcggaatatt gaaactgttg
     3061 cggataagat cgtgcttata ttttgcaata aagtagcaag tgaaatcaac acacatgcca
     3121 taagctatgc tgaataccaa tacggtacta ggttttatag ctatatgcat aaaacccatg
     3181 atggctcccg taatgaataa tggaattaag ttcgggagga atgaaatggc
//
LOCUS       DAOQOU010000256         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_22426_length_3180_cov_7.832320, whole genome shotgun sequence.
ACCESSION   DAOQOU010000256 DAOQOU010000000
VERSION     DAOQOU010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22426_length_3180_cov_7.832320"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..740)
                     /locus_tag="PLM90_14120"
     CDS             complement(135..740)
                     /locus_tag="PLM90_14120"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMZ90569.1"
                     /translation="MLQAYHQTELLEAGCDEAGRGCLAGPVVAAAVILPLDFYHPLLN
                     DSKQLSESTRNTLAPVIRENAIAWAIGICDQTEIDKLNILRASFAAMHKAINQLSVRP
                     QLLLIDGNRFHKYPGIPHLTMIKGDARFASIAAASILAKTVRDEMMQQLHVEYPVYNW
                     SSNKGYPTSEHRDAIRIHGDTKYHRKTFRLLPEQERELFGG"
     gene            831..1721
                     /locus_tag="PLM90_14125"
     CDS             831..1721
                     /locus_tag="PLM90_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ90570.1"
                     /translation="MEPVLVTVQLKKHFGPIKAVNGLDLAVQKGAVYGILGPNGSGKT
                     TTLGMLLGIVHPSGGDYSWFGNGQKDENRKRVGALLEQPNFYPYLSGEQNLKVVARIK
                     GVNPSEIDTVLAQVNLLESKKRQARTYSLGMRQRLALASALLGNPDVLVLDEPTNGLD
                     PQGIAEVRDFIRQQSAQGRTIILASHILDEVEKVCSHVSVIKKGNLLAQGTVNDLLKG
                     EDQVEIGAKDINALSNALEGYPGIRRLEKTNDLIVLHVNEAFDTAALNKFFSDKQIHL
                     SHLVGRKKRLEEQFLELTAE"
     gene            1723..2502
                     /locus_tag="PLM90_14130"
     CDS             1723..2502
                     /locus_tag="PLM90_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ90571.1"
                     /translation="MLRLLKIELHKLLPYRTFKILFIVYLLLMVLWLFAGKGLLSGGP
                     MTPSQILGFPNIWNYYALYACYFNIILAILIIFVTCNEYTYRTLRQNVIDGMQRNETV
                     LGKIYLIILLSIISTLVLSISGVIGGLIFSNADDKSKIFDHSGFIFAYLIQSLGMMSM
                     GYFFGTLFKRTGLAVIIFLIFLFPIDVILREGILPDGSGDYMPVATYFLKMVPWPVSH
                     MMSRGEEPTPGAPTLLAMSVGAAYAVIFTALGWLITKKRDL"
BASE COUNT          921 a          642 c          670 g          947 t
ORIGIN      
        1 tgatacttga tacttgatac ctgatacttg atacctgata cttgatacct gatacttgat
       61 acctgatact tgatacctga tacttgatac ctgatacctg atacctgata cctgctaacc
      121 atacccgaaa cccactaccc tccaaacaac tctcgttcct gttcgggtaa tagtctgaat
      181 gtttttctat gatattttgt atcgccatgt attcttattg cgtcgcgatg ttctgaggtg
      241 ggatagcctt tgttagacga ccaattatat accggatatt ctacatgcaa ttgctgcatc
      301 atttcgtcgc gaacggtttt tgccaatata cttgctgcgg caatactcgc aaatctggca
      361 tcacctttga tcatggtgag gtgcggaatg cccggatatt tgtgaaagcg gttgccatca
      421 attaataata attgtggtcg cacggataat tgattgattg ctttatgcat agcagcaaaa
      481 cttgcgcgaa gaatattgag tttatcaatt tcagtttgat cgcaaatacc aattgcccaa
      541 gcgatggcgt tttcacgaat aacaggtgca agcgtattcc gtgttgattc tgaaagctgt
      601 ttcgagtcgt tgagcagcgg atgataaaaa tcaagcggaa gaataacggc agcagcaacc
      661 accggaccgg caaggcaacc gcgacctgct tcatcgcagc cggcctccaa taattcggtt
      721 tgatggtatg cttgtaacat taaatattat tcatgcgtta tacatataca cgctttcggt
      781 atgataattg ccgaagtggt gattacattt acactcaaac ttaacacaat ttggaaccag
      841 tattagtgac cgttcagttg aaaaagcact tcggtccgat taaagcggtc aacggactcg
      901 accttgcggt gcaaaaaggt gctgtttatg gtatcctagg gccaaatgga agtggcaaaa
      961 ccactacctt aggtatgttg cttggcatag tgcatccttc gggtggcgat tattcatggt
     1021 ttggcaatgg ccaaaaagat gaaaaccgga aacgcgtcgg cgccttgtta gagcaaccga
     1081 atttttatcc ctacctcagt ggcgaacaga atttgaaagt tgttgcccgc attaaaggcg
     1141 ttaatccgtc cgaaatcgat actgttctgg cacaggttaa tttgctcgaa agcaaaaaaa
     1201 ggcaggcgcg cacttattca ttgggaatgc gtcagcgact ggcactggca tcagccttgt
     1261 taggcaatcc ggatgtactt gttctagatg aaccaacaaa tggtctcgac cctcagggta
     1321 ttgccgaagt acgtgatttt attcgccaac aaagtgcaca gggacgcacc attattcttg
     1381 ccagtcacat tcttgatgag gtagaaaaag tatgtagcca cgtaagcgtc atcaaaaaag
     1441 gaaatctgct tgcacaaggc accgtaaatg atttattgaa aggagaagat caggttgaga
     1501 ttggtgcgaa agacataaat gccttgagca atgcacttga aggatatccc ggcatacgcc
     1561 gtcttgaaaa aacaaatgat ttgattgtat tacacgttaa tgaagcattt gatactgcgg
     1621 cattaaataa attcttttcc gataaacaaa tacatctcag tcatctggta ggacgcaaaa
     1681 aacgacttga agaacaattc cttgaactaa ctgctgaata acatgcttcg attattaaaa
     1741 atagaattgc ataagctgct gccttatcgc acattcaaaa tactttttat tgtgtattta
     1801 ttgttgatgg tgctctggct gtttgccggg aaaggtctgc tatcaggagg tcccatgaca
     1861 ccatctcaaa ttctgggatt tccgaatatc tggaattatt acgcacttta cgcctgctat
     1921 ttcaatatca ttttagctat actgatcatt tttgtaacat gcaacgagta tacctatcgc
     1981 acattgcgtc agaatgtaat tgatggcatg cagcgcaatg aaacagtgct tggaaaaatt
     2041 tatctcatca ttttattaag catcatcagt actttggtat taagcattag tggtgtaatt
     2101 ggcggactaa ttttttccaa tgctgatgat aaatctaaaa ttttcgatca cagtggtttc
     2161 atcttcgcct atcttattca atcgcttggc atgatgagca tggggtattt tttcggaaca
     2221 cttttcaaac gcaccggatt ggcagttatc atttttctca ttttcctttt tccaattgat
     2281 gtgattttgc gcgaaggaat tttacccgat ggctccggcg attatatgcc tgttgccact
     2341 tattttctca aaatggtacc atggcctgtt tcacatatga tgagtcgagg agaagaacct
     2401 acacccggcg cacctacttt attggcaatg agtgtaggcg cagcatatgc tgttatcttt
     2461 acggcattgg gatggttgat cacgaagaaa agagatttgt aatagtgcct tgaaaggcct
     2521 cagaaaaagg cggagtgagc cggtttgcac cgattctctc cgcctttctg atttcagcct
     2581 tctctcttgc tttccataca ggggacaaaa aaaaccgcat cgaggagctg atgcggttta
     2641 gcgactctaa atatgagaac taaaaaaatt tggacttaac ccttgtcagt cgttaccctg
     2701 acgaagacaa aaatgcatca gtattgaaaa gtatcctaag acaaaaattt gagtttattg
     2761 tactcgtttt taggcctaaa aatacataat acactgattt acagccattt agacaatttt
     2821 tttacgcctg ttttgtggaa tttcatgcaa ttatcggatg acatttatcc gataaatcag
     2881 gctgattttc ctcataaata ctcaaaagtg gccaaaaact cacccgataa atgcaaaaat
     2941 gggggcttat cggaaagcat ttgactgaaa cctattaagg caataagatt gctttaggtc
     3001 attttgaatg gtatgctccc atacgagaca aaaaaacccg catcgaggag agatgcgggt
     3061 tgaggagcga aacagatgat tgatcagccg gatcttttac tgttttacaa attgctcagt
     3121 ggcaatgttg ctgccaaaat tatattcaac gatgtaagtt cctgctgcga gtttacttac
//
LOCUS       DAOQOU010000257         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_22935_length_3138_cov_4.206941, whole genome shotgun sequence.
ACCESSION   DAOQOU010000257 DAOQOU010000000
VERSION     DAOQOU010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22935_length_3138_cov_4.206941"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..721)
                     /locus_tag="PLM90_14135"
     CDS             complement(146..721)
                     /locus_tag="PLM90_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HMZ90572.1"
                     /translation="MQEIYAAVIMPPEEIAETIRIFKDGLAGVVGKYSSSASDPVIHL
                     FSAVGDNERISQLEEKLEVLCRNQLPFNVHLDHFDEFHSHKTVFVDISETSKKAITSL
                     RKKLSSELKSSKYDEEVDFSVGKTPHITIARNLPDDQYRVARNYFTGRTYDAYFECSS
                     ITWRKLIRSNNNTYYETVREFPFAMKNLSLF"
     gene            complement(723..2240)
                     /locus_tag="PLM90_14140"
     CDS             complement(723..2240)
                     /locus_tag="PLM90_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMZ90573.1"
                     /translation="MKKIYSLIFALCVATLTFAQQPAFITDSLDSYIQREMKKWNIPG
                     MAVAIVKDGQIVVEKGYGVTDVHTGQKTDQNSLFMVASNSKAFTGTALSLLEYQKRLN
                     LNDKVTTYLPYFKLYDPNASQLVTVEDLMCHRLGLETFQGDFLNWDGNLSREQVVRAM
                     ALHKPSFEFRDTYGYCNAGFVAAAEVLYAVTDTTIDDFLKAHIFTPLQMTRTSTTHAA
                     IAADKNACKPYTVFDGRLVQLEYDNVDNLAAAASINSSAHDLANWLIMQLNNGKFNDV
                     QVLPKQVVNNPRLPRTVVGSGNSSIYKSQHFNLYGLGWFIKDFEGRRVYSHDGGANGF
                     VTGTTFIPEAGLGIVVLTNTDANAFYDALRYQIMEAYFNVPYRNISDIYLARVTPGNE
                     AQDSVLHRYQAIAEKNLTPALPIASYTGKYHNEVYGDMEIKESNGTLVMTFSHHPQLT
                     GSLRASGGNNFICFFDPVSWGVKEFPFTVVDGKVKSVTVTINDFIDFMPYEFVKQ"
     gene            complement(2237..2863)
                     /locus_tag="PLM90_14145"
     CDS             complement(2237..2863)
                     /locus_tag="PLM90_14145"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMZ90574.1"
                     /translation="MSEISRIPLLLEPHYFPCISWWAAVSRHESVVLDVSAHYQKGSY
                     RNRAHILGANGVMRLSVPLEHGRTQRKTTGDVKICYDEDWQKIHWQTLISSYRRSPFF
                     EYFEDILEALFVRPFTFLIDMQMAVIAIMKKLIGIETQFTLSTHYIDPGDPQYVDLRN
                     TIHPQKTSQLNFIRYTQVFSDRFAFIPDLSIIDAICNKGKITINDLIL"
     gene            2917..>3138
                     /locus_tag="PLM90_14150"
     CDS             2917..>3138
                     /locus_tag="PLM90_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90575.1"
                     /translation="MRNLYLFTILICLTGFSAQAQSLSTNTAVRSCLFLHPTEGAFAE
                     VYLVAGANALRPAAGNNGFTAGVDVSILVI"
BASE COUNT          859 a          678 c          644 g          957 t
ORIGIN      
        1 cgctaactcc gcaaactccg cgattaaaca agggagaatc gtgaatcgtg aaatgccact
       61 tacgccttac caccaaactt ttgcgcctta gctcctttgc gcccgccttg ccggccggca
      121 ggcctttgcg cgaaaaagca cacaactaaa ataaagacaa atttttcatc gcaaatggaa
      181 actcgcgaac ggtttcataa taggtattat tgttacttcg tattagtttc cgccatgtga
      241 tggagctgca ttcgaaataa gcatcataag ttctgcctgt aaaataattt cttgccacgc
      301 gatattgatc gtcggggaga ttgcgtgcga tagtgatatg cggagttttt cccacgctga
      361 aatcaacttc ctcatcatac tttgaagatt tgagttctga agaaagtttt tttctcaatg
      421 aagtgattgc ctttttcgat gtttcagaaa tatcaacaaa caccgtttta tgcgaatgaa
      481 actcgtcgaa gtgatccagg tgcacattaa acggcaattg atttcggcac agcacttcta
      541 atttttcttc cagttggctg atacgctcat tatcgcctac tgcagaaaat aaatgtatca
      601 ccggatcgga agcactgctg ctgtatttgc cgactacacc cgccaatccg tccttaaaaa
      661 tgcgaatggt ttcagcaatt tcttcgggtg gcatgattac tgccgcatag atctcctgca
      721 tattattgtt ttacaaattc atatggcatg aaatcaataa agtcattaat cgtaaccgtc
      781 accgatttta ccttcccgtc aacaacagta aaaggaaatt ctttcacgcc ccaggacaca
      841 gggtcgaaaa agcaaatgaa attatttccg cctgatgcac gcaaagaacc tgttaattgc
      901 gggtggtgac tgaatgtcat taccagtgtt ccatttgatt cttttatttc catatcgcca
      961 tacacttcgt tgtgatattt tccggtgtaa gatgcgattg gtaatgcagg tgtgagattt
     1021 ttttctgcaa tggcctgata tctgtgtagc acggaatcct gcgcttcatt tcccggcgta
     1081 acccttgcca gatagatatc agaaatattg cgataaggaa cattgaaata cgcttccata
     1141 atctgataac gcagggcatc ataaaaggca ttggcatcgg tatttgtaag cacaacaata
     1201 cccaaaccgg cttccggaat aaatgttgtt ccggttacaa acccgtttgc gccaccatcg
     1261 tggctataca cgcgtcgccc ctcaaaatct ttgataaacc agcccagtcc gtataaatta
     1321 aaatgctgtg atttataaat acttgaattt cctgaaccta ctactgttcg aggcaagcgc
     1381 ggattgttca ctacctgttt gggcagcact tgcacgtcgt taaatttgcc gttgtttaat
     1441 tgcatgatca gccaatttgc caaatcatga gcagatgaat ttatggaagc tgctgctgcg
     1501 agattgtcta cgttgtcgta ttctaattgt acaagtcgac catcgaaaac ggtatatggt
     1561 ttgcatgcat ttttatcagc agcgatagca gcatgcgttg tgcttgttcg cgtcatctgc
     1621 aacggagtga aaatatgtgc tttcaggaaa tcgtcaatcg tagtatctgt aacggcatat
     1681 aatacttcag ccgcggcaac aaatccggca ttgcaatatc cataagtatc gcgaaattca
     1741 aaagaaggtt tgtgtaatgc cattgctcgc accacttgtt cgcgacttaa attgccatcc
     1801 cagtttagaa aatcaccttg aaatgtttca agcccgaggc gatgacacat caagtcttca
     1861 acggtaacca gctgacttgc attcggatca tagagtttaa aataggggag ataagtagta
     1921 accttatcat tcagatttaa tcgcttttga tattcgagca gcgataatgc cgttcctgta
     1981 aatgctttgc tgttactggc aaccatgaac agcgaatttt gatcggtttt ttgtccggta
     2041 tgcacatcgg taacgccata tcctttttct acaacaattt gtccgtcctt aacaatggct
     2101 actgccatgc ccgggatatt ccattttttc atttcgcgct ggatatagct atccaaacta
     2161 tcggtaataa atgcgggttg ctgagcgaat gtcagtgtag caacacatag tgcgaaaatc
     2221 aggctatata tttttttcat agtatcagat cgttaatggt gattttacct ttattacata
     2281 tggcatcgat aatactcagg tcgggaataa atgcaaatct atcactgaat acctgcgtat
     2341 agcgaatgaa atttaattgc gatgtttttt gtgggtggat ggtattcctc aaatctacat
     2401 attgtgggtc gcccggatcg atataatgtg ttgaaagggt gaattgcgtt tcaataccta
     2461 tcaatttttt cattattgca atcactgcca tttgcatgtc gatgagaaat gtaaatggtc
     2521 tgacaaacaa tgcttctagt atatcctcaa aatattcaaa gaaaggggag cggcggtaac
     2581 tgctgatgag ggtttgccaa tgtatcttct gccaatcttc atcataacag atttttacat
     2641 ctccggttgt tttacgttgt gttctgccat gttcaagtgg tacacttaat cgcataacgc
     2701 catttgctcc gagtatatgc gctctgttgc gatagcttcc tttttgataa tgtgcagaaa
     2761 catccaaaac cactgattca tggcgcgata ctgctgccca ccacgatatg caagggaaat
     2821 agtgcggttc taacaatagt ggtatacggg aaatttctga catcgttgtg acgtgtgtat
     2881 ctaaatatgc tgtaatttta agattatttt tcggctatga gaaacctata cctgttcact
     2941 atcctcattt gtttaaccgg cttttctgcg caagcacagt cactgtcaac caataccgct
     3001 gtcagaagtt gccttttcct gcatcctacc gaaggcgcct tcgcagaagt atacctggtt
     3061 gccggagcca atgccttgcg tcctgcagcc ggaaataatg ggttcactgc gggtgttgac
     3121 gtatccatat tggtgata
//
LOCUS       DAOQOU010000258         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_23864_length_3070_cov_5.667330, whole genome shotgun sequence.
ACCESSION   DAOQOU010000258 DAOQOU010000000
VERSION     DAOQOU010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23864_length_3070_cov_5.667330"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLM90_14155"
     CDS             <1..638
                     /locus_tag="PLM90_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HMZ90576.1"
                     /translation="AHYLNDENARKTIESGLDRLIISIDGTTQDVYQQYRVGGNLEKV
                     LEGARNIVKWKKEMRSKTPFVIFQFLVVKPNEHQIEDVKKLGKEIGVDEVAFKTAQIY
                     DYENGNDLIPTIDYYSRYAKQDDGKYRIKNKMLDHCWKMWHSCVITWDGAVVPCCFDK
                     DAEHKLGDLKQQTFSELWRGPAYQHLRSSLLRSRSEIDICKNCTEGTKVWA"
     gene            646..1824
                     /locus_tag="PLM90_14160"
     CDS             646..1824
                     /locus_tag="PLM90_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMZ90577.1"
                     /translation="MHHRQLFLRHVAQTSDAPLAIEITQAAGNWLWDADGKKYLDLIS
                     GIAVSNIGHGHSAVKSAINDQVDKYMHVMVYGEIVQSPQVQYATWLTNHLPSTLNSVY
                     FVNSGSEAVEGAIKLAKRVTGKTEIISFRNSYHGSTMGALSVGNTEERKNAFRPLIPD
                     NTVLTYNDPAAIQAISSRTAAVIIEPIQAEAGVITPNSEFLQQLREACSTHDAMFILD
                     ECQTGFGRTGTLFAFEQMNLVPDMLVLGKAVGGGMPLGCFIADIASMQAFTHDPVLGH
                     ITTFGGHPVCCAAGLAAAQVLEDEKLMEHIPNLLQVFTSKLQHPKIKSFRHAGLLMAV
                     EFYSVEDNFSIIKKLIDKGLFVDWFLFATDCMRLAPPLTLTRHEAEHACEIILAVLNE
                     "
     gene            1876..2865
                     /locus_tag="PLM90_14165"
     CDS             1876..2865
                     /locus_tag="PLM90_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMZ90578.1"
                     /translation="MKKLLFTILILGATAQFAAAPEPMSAPWGFWGHQRINKMALFTL
                     PEDLFGYYKPNIDYVTAHAVDPDKRRYAFADEAPRHYIDLDHYGKPPFENVPRKWNDA
                     VVKYSEDSLMDHGIVPWHIERMMYRLTEAFKQKDMNYILKTTADLGHYVADAHVPLHC
                     TENYNGQLTNQVGIHGFWESRIPELLGEDFDYYTGKAEFIPDVNAKVWEIVLESNSLV
                     DSVLSLEKILSTEFPADRKYAYETRGASTVRVYSKEYTERYNEMLDGMVERRMRESII
                     TLGSFWLTAWINAGKPNLTGIVMRPWTADELKEMEELDASYSKGTLKGREHEE"
BASE COUNT          925 a          587 c          712 g          846 t
ORIGIN      
        1 acgcgcatta tctcaatgat gaaaatgcgc gaaaaactat tgaaagtggt cttgatagat
       61 taattatttc tattgatggc actacacagg atgtttatca gcaatatcgc gtaggaggaa
      121 atcttgaaaa agtgctggaa ggtgcacgca atattgttaa gtggaaaaaa gaaatgcgct
      181 cgaaaacacc atttgttatt tttcaatttc tcgtggtaaa accgaatgaa catcaaattg
      241 aagatgttaa aaaattggga aaagaaattg gtgttgatga agtggccttc aaaactgcac
      301 agatctacga ttatgaaaat ggcaacgatc tgattcctac tatcgattat tattcacgtt
      361 atgccaagca ggatgatggt aaatatcgca tcaaaaataa aatgctcgat cactgctgga
      421 aaatgtggca cagctgtgta atcacctggg atggcgcagt agtaccttgc tgcttcgata
      481 aagatgcaga acataaactc ggcgacctca agcaacaaac attttctgaa ttatggcgcg
      541 gaccggccta tcaacatttg agaagctcct tgttgcgttc gagaagtgaa attgatattt
      601 gtaaaaattg caccgaaggc accaaggtgt gggcttaatt attatatgca tcatcgccaa
      661 ttatttttga gacatgtcgc ccaaacaagc gatgctccgc ttgccatcga aattacacag
      721 gcagcgggta attggttgtg ggatgccgat ggaaaaaaat atctcgacct tatttccggt
      781 atcgcagtga gcaatattgg tcatggtcac tcagcagtta aatctgccat caatgatcag
      841 gttgataaat atatgcacgt gatggtgtat ggtgaaatag tgcaatcgcc acaagtgcaa
      901 tatgctacat ggttgacgaa tcatcttcct tcaacattaa attctgttta tttcgtcaac
      961 tcaggttcag aagctgttga aggagcaatt aaattggcca aacgcgtgac aggaaaaaca
     1021 gaaataatta gttttcgcaa ttcctatcat ggcagtacca tgggtgcatt gagtgttgga
     1081 aataccgaag aaagaaaaaa tgccttccgc ccgctaattc ccgataatac tgtccttact
     1141 tacaacgatc ctgcagccat acaagcgatc tcttcgcgaa cagcagctgt gatcattgaa
     1201 ccaattcagg cagaggccgg tgtaataacc ccaaattccg aatttttaca acaattacgc
     1261 gaggcttgtt ctacgcatga cgcaatgttt attttagatg aatgtcagac cggtttcggt
     1321 cgcacgggta ctttgtttgc attcgaacaa atgaacctcg ttcccgatat gttagtgctt
     1381 ggaaaagctg taggtggagg tatgcctttg ggatgtttta ttgcagatat tgcaagcatg
     1441 caagcattca cccatgatcc cgtgctcgga catatcacta ctttcggagg tcaccctgta
     1501 tgttgtgccg cgggattggc tgcagcgcag gttttggaag atgaaaaatt aatggagcat
     1561 attcccaatc ttctgcaggt ttttacatcg aaattgcaac atcctaaaat caagtctttc
     1621 cgacacgccg gccttctgat ggccgttgaa ttttattcag tggaagacaa tttcagcatt
     1681 attaaaaagc tcatcgacaa gggattattc gtcgactggt ttttatttgc caccgattgc
     1741 atgcgtctgg cgccaccgct cactctaacc aggcatgaag ctgaacacgc ctgtgagatt
     1801 atccttgccg ttttaaatga gtgaccacat tttttcatct gacggctatt gattatcctt
     1861 gtattgtaat tcgtcatgaa aaaattgctg tttaccatac tcatcctcgg cgcaactgct
     1921 cagtttgccg ctgctcccga accgatgtcg gctccctggg gtttttgggg acatcaacgc
     1981 atcaataaaa tggcgctgtt tacattgccg gaggatcttt ttggttatta taagcctaac
     2041 atcgattatg taactgctca tgctgtcgat ccagataaaa ggagatatgc atttgccgat
     2101 gaagcacctc gccattatat cgatttggat cattatggca aaccaccttt tgaaaatgta
     2161 ccgcgaaaat ggaatgatgc agttgtaaaa tattctgaag actcattaat ggatcatggt
     2221 atcgttccat ggcatattga acgcatgatg tatcgcttaa ccgaagcgtt taaacaaaag
     2281 gatatgaatt atatcctcaa aacaactgcc gatctcggac attatgttgc ggatgcacat
     2341 gtgcctttgc attgcacaga gaattataat ggtcaactta caaatcaggt tgggatccac
     2401 ggtttttggg aatcgcgtat tcctgagttg ctgggagaag attttgatta ttatacaggc
     2461 aaggcagaat ttattcccga tgtaaatgca aaagtttggg aaattgtact ggaaagcaac
     2521 tccctggtcg atagcgtcct gtcgcttgaa aaaatattga gcactgaatt tccggccgat
     2581 agaaaatatg cgtacgaaac aagaggtgct tcaacggtaa gagtttattc aaaagaatat
     2641 accgaacgct acaatgaaat gctcgacggc atggtagagc gacgaatgcg agaaagtata
     2701 atcactctag gcagtttctg gctaacagca tggattaatg ccggcaaacc caatctgaca
     2761 ggaatcgtca tgcgcccgtg gacggcagat gaattaaaag aaatggagga gcttgatgca
     2821 tcatactcaa agggaacctt gaaaggccga gagcatgagg aatgattggg gaggagcaag
     2881 gagagaggag gaaggagaaa ggagaaagga gaaatggtgt gtgtgggggt aattagcaaa
     2941 tttgagaatt agcaaattag ctaattggga atgtgcttgc atgaagacgc tcgtcttagt
     3001 tagcaagtgt gatagttagc aaattagcaa gtgtggagtg cttttaacgc tggcgcgtta
     3061 atagggaatg
//
LOCUS       DAOQOU010000259         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_24596_length_3018_cov_5.924738, whole genome shotgun sequence.
ACCESSION   DAOQOU010000259 DAOQOU010000000
VERSION     DAOQOU010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24596_length_3018_cov_5.924738"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLM90_14170"
     CDS             complement(<1..645)
                     /locus_tag="PLM90_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90579.1"
                     /translation="MKRTFKHVIFSLLMLCVAHASYAATITVLPGNGSTSGNGRAPQG
                     SRLYINTLYYISPSEMTTSGYGSDQVTTVGWTWQAGSPQNVTTTGSLKVYLQNSADLA
                     YSKGTTFSTVGMTKVIDGTITITNSGVQFSIDVPVGGTGTSVFNTVAGQGLYVAFEYQ
                     TTTALALPLGAPTVLCTNTVANSLGTYQSQTAYGTPMTLSGFRPETRLGNPLSDK"
     gene            910..2412
                     /gene="cysS"
                     /locus_tag="PLM90_14175"
     CDS             910..2412
                     /gene="cysS"
                     /locus_tag="PLM90_14175"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMZ90580.1"
                     /translation="METALFHQNKLMVYNTLSREKEHFIPLHAPFVGMYACGPTVYSE
                     PHLGNCRSFVMYDLMVRYLTHLGFKVRYLRNITDAGHVESTSGETGDRIGKQARLEQV
                     EPMEIVQKYTVKFHWIMDLLNCLPPSIEPTATGHIVEQIEMTKTILANGYAYETDEAI
                     YFDVEKFSKDYPYTQLSGRNLEELLNNTRELGGQSSKKGRLDFALMIKAKPEHIMRWP
                     SPWGDVYPGWHIECSAMSTKYLGKTFDIHCGGMDLIPTHHTNEIAQSIACNHTTPVKY
                     WLHPNMLTVNGQKMSKSLGNAFLPVELFEGTHPLLDKGYSPMTVRFSMLQAHYRSTLD
                     FSNDALKAAEKGYKRLMSAMNLLKGITVSDTVNADEEAAVNKILLGVYEGMNNDFNAA
                     QAIAELNEAATIINTWNDKQNKKASIQAFTLSRLQQTYRAFLIDIFGLQEETENDNSK
                     LSGVMDLVIDIRKDARGKKDFATSDKIRDALQSVGIQLKDSKDGTTWEGN"
     gene            2541..>3018
                     /locus_tag="PLM90_14180"
     CDS             2541..>3018
                     /locus_tag="PLM90_14180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ90581.1"
                     /translation="MSQTPFDLAAPEYDSRFTETPAGKAQRDMVWRFLHKQLPPSPAN
                     ILEINCGTGEDAVHLATAGFHVTATDASENMLAEAGKKFASVRMNVHSYVWDVRTPFI
                     GTEKFNVIFSNFGGLNCLSPSELRSLFKRMQSQLSENGKLIFVVMGRNCPWERLLFS"
BASE COUNT          931 a          576 c          679 g          832 t
ORIGIN      
        1 cttatcagac aatggatttc ctaagcgagt ttcagggcgg aaacctgaca atgtcattgg
       61 cgtgccataa gcagtttggc tctgataagt acctagtgaa ttagctacag tattagtaca
      121 taataccgta ggcgcaccta aaggcaatgc aagtgcagta gtagtttgat attcaaaggc
      181 tacatataaa ccttgtccgg ctactgtatt aaatacagaa gttccggtac caccaacagg
      241 aacatctata ctaaattgaa ctccggagtt cgtaatagtg attgttccat caataacttt
      301 agtcataccc acagtggaga aggtagttcc tttgctatag gcaagatctg ccgagttttg
      361 caaatacacc ttcaagctac ctgttgtagt aacattctga ggcgaaccgg cctgccaggt
      421 ccatccaaca gttgtaacct gatcagagcc ataacccgaa gtagtcattt cggaaggcga
      481 gatataatat aatgtgttaa tgtaaaggcg cgatccttga ggtgcgcgac cattaccgga
      541 tgtagatccg tttcccggca aaaccgtaat tgtggcagcg taagatgcat gcgccacaca
      601 tagcattagc agggaaaaga tgacgtgttt aaacgttctt ttcataataa ttggattttg
      661 ggtgaaggaa aaaattgttg aaaagaccac cgacaggtag cccattagaa tgtaaaatga
      721 tgctcgtacg ttgtgacatt tgtgtgtgtt tcaagtaccg ggatcagggc ttgctggggg
      781 cagagacatg atcaaccgaa atgaaaatta ggagtaaaat tcaaattttc aaatttttca
      841 caaatatttt ttcaggaggt agtgatacct gcctaggctg attcggcaat aacctgctac
      901 ctttggggca tggaaacagc cttattccat cagaacaaac tcatggtgta caacactttg
      961 agccgtgaaa aagaacattt tattccgcta catgcgccat ttgtaggcat gtatgcctgc
     1021 gggcctaccg tttatagtga accgcatctt ggaaattgcc gaagctttgt gatgtacgac
     1081 ctgatggtaa ggtatcttac ccatttgggc tttaaagtgc gctatttgag gaatattacc
     1141 gatgctgggc atgttgaaag caccagtgga gaaaccggcg acaggatagg aaaacaagct
     1201 cgattggaac aagtggagcc gatggagatc gtgcagaaat acacggtgaa atttcattgg
     1261 attatggatc tattgaattg tttacctccc agcatagagc caactgcaac aggtcatatt
     1321 gttgaacaaa ttgaaatgac gaaaacaatc ctggcaaatg gatatgctta tgaaacggat
     1381 gaagccattt attttgatgt cgaaaaattt tcgaaagatt atccatatac acaactcagc
     1441 ggacgcaacc ttgaagaact gctgaataac acgcgtgaac tcggcggaca atcaagtaaa
     1501 aaaggacgat tggattttgc gcttatgatc aaagctaaac cagaacatat tatgcgctgg
     1561 ccttcgccgt ggggtgatgt atatcccggt tggcatattg aatgttctgc catgagtacg
     1621 aaatatctgg gcaaaacatt tgacattcat tgcggtggca tggatttaat tcctacacat
     1681 cataccaatg aaattgcgca gagcattgca tgcaatcata ccactcctgt aaagtattgg
     1741 ctgcatccga atatgctcac tgtcaacgga caaaaaatga gtaagagttt gggcaatgca
     1801 tttttgccgg tagaattatt tgaaggcacg catccattgc ttgataaagg atattcacca
     1861 atgacggtgc gtttttcgat gttacaggca cattatcgca gcacacttga ttttagtaat
     1921 gatgcattga aggctgctga aaaaggatac aagcgcttga tgtcggcaat gaatttatta
     1981 aaaggcatta cagtatccga tactgtcaat gccgatgaag aagccgccgt aaataaaata
     2041 ttgcttggtg tttacgaggg tatgaataat gattttaatg cggctcaggc aattgcagaa
     2101 ttgaatgaag cggcaactat catcaacacc tggaacgata aacaaaataa aaaagcatcg
     2161 atacaggcat ttacgttaag cagactgcaa caaacctatc gcgcatttct tattgacatt
     2221 tttggattac aagaggagac agaaaatgac aatagcaaat taagtggtgt aatggacctt
     2281 gtaatcgaca ttcgcaaaga tgcacgtgga aagaaagatt ttgctaccag cgacaaaatt
     2341 cgcgatgcgc tgcaatctgt gggaattcaa ctgaaggata gtaaggatgg gacaacgtgg
     2401 gaggggaatt aatttcctct aatgatgatt tcttcgaata ttcacattaa ttaaacacgt
     2461 tatttccacc atttacataa gctatcttcc atttagtctt gctattcagg taaatgtttg
     2521 aatttatgta ggtttgcctt atgtcgcaaa caccttttga cttagcagct cccgaatacg
     2581 acagccgttt cacagaaact cccgcaggca aggctcagcg agatatggtg tggcgatttt
     2641 tgcataaaca gcttccacct tctccggcca acattctcga aatcaattgc ggcacaggcg
     2701 aagatgccgt gcacctggca acagcgggat ttcatgttac tgcaacagat gcatcagaaa
     2761 atatgcttgc cgaagccggc aagaagtttg catcagtacg aatgaacgtt cattcatatg
     2821 tatgggatgt cagaactcca tttattggaa ctgaaaagtt taatgtgata ttttcaaatt
     2881 ttggcggatt gaattgttta tctccttccg agttgcgcag tttatttaaa aggatgcaat
     2941 cgcaattatc tgaaaatggg aaattaattt ttgtcgtgat gggtagaaat tgcccatggg
     3001 agcgattgct tttttcat
//
LOCUS       DAOQOU010000260         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_25209_length_2978_cov_6.081423, whole genome shotgun sequence.
ACCESSION   DAOQOU010000260 DAOQOU010000000
VERSION     DAOQOU010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25209_length_2978_cov_6.081423"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1573
                     /locus_tag="PLM90_14185"
     CDS             188..1573
                     /locus_tag="PLM90_14185"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HMZ90582.1"
                     /translation="MKTGIILFLIMYSGLVNAQMTITTLPDDNTEHAGTWLQWPHHLT
                     YGTIYRDRMEPCFTAMTAALVPTEHVNIIAYNNTEKNRIISVLNANAVPLANITFYII
                     QTNDCWARDNGPMYVYTNAGQLKILDWGFDGWGNDAPYTKDNAVPKKISDQTAVPRIN
                     LSAMELEGGAVCVDGAGTFLATRSSIQGDGRNPALTETDINNYLIQYLGVSNIVWLDG
                     LYGGGLDITDTHIDGFATFLDSTTLVTLSNSDLSYWGITSADRTILNNMTNANGNVYT
                     KVILPLTNKNVKTTWGANVGFKGSYNNYYVCNSVVLVPTYSDVNDATALSIIQSLYPS
                     KTVVGIDCRNAYFYGGMVHCLTQQQPVNPTPRMEYCASTENNAHLTIYPNPASDNIIV
                     QINSNDGQTVRISILDMNGKTMLEMPAYALLEGNNQIDLQVSWLPAGLYMVRISGDVE
                     TQRNGVFQVVH"
     gene            complement(1703..2548)
                     /locus_tag="PLM90_14190"
     CDS             complement(1703..2548)
                     /locus_tag="PLM90_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90583.1"
                     /translation="MKEDLEAIKHLQNNTPIEAFEGLTPSEMHCLLYNVYDKESSLQM
                     VDTIDDAILDQIPIFRIAEVFLHIIQRENGIKLTLGGSLPVKVLTELYNYGYILEDFI
                     EKGYVKLRREQDCAAILSARLTVELTGLVKRTNGKLTLTKAGIKLLNKYDRNKFFHLF
                     MQAFTNKFAWSCNDGYGEDPVAQFGWAFSVILLDKYGDKPQEAIFYSEKFLNAFPNSV
                     LFFQPGLSTPEHFYASCYELRTFHRFFEWFGFVNVEGQHNLLTREFKAYTKSEIINKI
                     FRIVQ"
     gene            complement(2803..2970)
                     /locus_tag="PLM90_14195"
     CDS             complement(2803..2970)
                     /locus_tag="PLM90_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HMZ90584.1"
                     /translation="MIKHCCSELTYKEIADKMSVSQRTVDGYRESVFSKLDLKSRTGI
                     VLFAVHQGWLG"
BASE COUNT          934 a          580 c          560 g          904 t
ORIGIN      
        1 catttctaaa tgtatttgag atacaaggta agaaaaaaat cacgccgcaa caatttcaat
       61 tctaatttta tggatactag gttttaacag actaaatcat gtaaaaataa gatgattatt
      121 gaaagacaat ggtcaatagc attcacgtca atgataaaat tcaagtaatt catgctatat
      181 ttaaatcatg aaaaccggaa ttatcttatt ccttatcatg tacagtgggc tggtaaatgc
      241 acaaatgacc attaccacac tgcctgacga caatacagaa cacgcaggta cctggttaca
      301 gtggccacat catttaacgt atggcactat ttatcgcgac cgcatggagc cttgctttac
      361 ggcgatgacg gctgcacttg tgcctacgga gcatgttaac ataatcgctt ataataacac
      421 agaaaaaaac aggatcatca gcgtgcttaa tgccaatgcg gttccgctgg ccaatattac
      481 gttctacatc attcaaacaa atgattgctg ggcgagagat aatggtccga tgtatgtgta
      541 tacaaatgcc gggcaattaa aaatattgga ctggggtttt gatggttggg gaaacgatgc
      601 tccatataca aaagacaatg ctgttccgaa gaaaatctca gatcaaaccg ctgttccgag
      661 aattaattta agcgcgatgg aacttgaagg aggagccgtg tgtgtggatg gagccggtac
      721 ttttttagca acacgaagca gtattcaagg cgatggcaga aatccggcgc ttactgaaac
      781 agatatcaat aattatttaa ttcaatatct cggtgtaagc aatatcgttt ggcttgatgg
      841 attatatggc ggtggacttg atatcacaga tacgcatatt gatggcttcg caacgtttct
      901 cgacagcacg acactggtta cattgagcaa ttcggatttg agttattggg gaattacctc
      961 cgccgacaga accattttaa ataatatgac taatgcaaat ggcaatgtgt atacaaaagt
     1021 tattctgcca ctcaccaata aaaacgttaa aaccacctgg ggtgcaaatg tcggtttcaa
     1081 aggcagttat aataattatt acgtttgcaa cagcgtagtt ctggtgccaa cttacagcga
     1141 tgtaaatgac gccactgcat tgtccatcat ccagagtttg tatccaagca aaacagttgt
     1201 cggaattgat tgtcgcaatg catatttcta tggtggaatg gtgcattgtt taacgcaaca
     1261 acaacccgtt aatccaactc cacggatgga atattgcgca tcaacagaaa ataatgcaca
     1321 tttgacgata tatccaaacc cggcctctga taatattatt gttcaaataa atagtaatga
     1381 tggacagaca gtgcgaattt ctattttaga catgaatggc aaaaccatgc ttgagatgcc
     1441 ggcatacgca ttgcttgaag gcaacaatca aatcgatctt caggtatcat ggcttccggc
     1501 aggattatat atggtgcgaa tttccggtga tgttgaaacg cagcggaatg gggtgtttca
     1561 agttgtgcat taaagattta gcgcaatttc ttgtttaaat agattatata aactttgcct
     1621 aaatacaaaa caaaactgat tgaatattca atcatgtata catcgtatta ttataaatta
     1681 atcaagcagg aggaattgtt ttttattgca caatgcgaaa tattttattg attatttcgg
     1741 atttggtata agctttaaac tctcgcgtta acaaattgtg ctgaccttct acattgacaa
     1801 aaccaaacca ttcaaaaaaa cgatgaaatg ttcttagttc gtagcaactt gcataaaaat
     1861 gttcaggggt agaaaggcct ggttgaaaaa acaacacact attaggaaaa gcgttcagaa
     1921 atttctccga gtaaaaaatc gcctcttgcg gtttatcacc atacttatcc aacagaatta
     1981 ctgaaaaagc ccatccaaat tgtgccacag ggtcttctcc atatccatca ttacaactcc
     2041 aggcaaattt atttgtaaag gcttgcataa ataaatgaaa gaacttattc cggtcatact
     2101 tattcagcag ttttattcct gcttttgtga gggttaattt tccatttgtt ctctttacca
     2161 atccggtcag ctccacggtt agtctggcac tcaaaattgc agcacaatct tgttctcgtc
     2221 gcaactttac ataccctttt tcaatgaagt cttcaagaat atacccataa ttatatagtt
     2281 ctgtcaaaac ttttactggc agagaaccac caagtgttaa ttttattcca ttttctcttt
     2341 gaataatatg caaaaaaacc tccgcaatac ggaatatagg tatttgatcc aatattgcat
     2401 catctattgt gtcaaccatt tgcagagaag attctttgtc atatacatta tataacaaac
     2461 aatgcatttc actaggggtg agtccttcaa acgcctcaat cggtgtatta ttctgcaaat
     2521 gcttgattgc ttctaaatcc tctttcataa ccactcaatt acgcctaatc aaaatacgtt
     2581 taacaattta ggtttctaaa ttaagtatat tttttgattg catttcttta cgaacaatta
     2641 atatacgtgc cccgtttttc acgcggatcc cgatagctat cgggagcgga ggagcggaga
     2701 aatacaagtt tttcacgcag agagcggaga aatacaaaag ttaattgttt ttattattcc
     2761 gcattgttca ttccttattc ttcccctcac ttctcacgtt cctcacccca accatccctg
     2821 gtgcacggcg aataacacta ttcctgtgcg ggattttaaa tcgagtttgg agaacaccga
     2881 ttcgcggtag ccatcaacgg tgcgttggct gacggacatt ttgtctgcta tttctttgta
     2941 ggtgagttcg gagcagcagt gtttgatcaa ttcttttt
//
LOCUS       DAOQOU010000261         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_25405_length_2965_cov_6.165979, whole genome shotgun sequence.
ACCESSION   DAOQOU010000261 DAOQOU010000000
VERSION     DAOQOU010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25405_length_2965_cov_6.165979"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..1242)
                     /locus_tag="PLM90_14200"
     CDS             complement(313..1242)
                     /locus_tag="PLM90_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90585.1"
                     /translation="MICKVTKCLVLLLVVFGIQQLKAQVETSPVQIITVDSFVVHAVR
                     DGLNVQDFVQMIQEDTTFYRAFQNLRRIEYDGSATVRMFNEDHLSIASYSNRMSQFLD
                     GKCRWMQFPFEVNTGDFFDKHKQLNYYTAGVFSYIFLYRDTICGNIVETDNNAGSDDK
                     LELRKEQLKTLIFNPGRPVNGIPLIGDKMEIFSPEMQQYYDYTIAREKFATGVDCFVF
                     TIKKKPEAEKGKDVVINELTTWFDVDSKEIVARNYQLSYFTSLFDFDVSMKVRMGTID
                     GYRVPVSIKYSGYWDIPFRDPEIGNIDISVWGR"
     gene            1263..>2965
                     /locus_tag="PLM90_14205"
     CDS             1263..>2965
                     /locus_tag="PLM90_14205"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ90586.1"
                     /translation="MLREKWFLLILTACMQQAYAATPTISDSIDIRHYDINLDITDYA
                     GQTINGSTTLHIAALVDGITTLPLDLLSLDVDGITDGNGNALSFTYDEPSLIITLFTP
                     LNIGDSANIIIQYSGDPAQDGSWGGWYWSGDYSYQLGVGFDAIPHNYGRAWFPCFDNF
                     IERSTFSYDIKTTNDKKAFCGGLLIDSVDNGDGTISWHWECVQQIPSYLASVAVSKYA
                     TINDVYAGIAGDIPIQLACKAEDTTDMKNSFIHLHDALSTFENHYGPFRWDRVGYAVV
                     PFSGGAMEHAMNIAYPLFAVNGTTLWENLYAHELSHHWWGDLTTCSTPQEMWMNEGWA
                     VFSEHLFQESLYGDQAYMDAVKANQLDVLHNAAPRDGNNYFAISGVPESYTYGATTYN
                     KGASVIHTLRGYMGDDAFFSCITSFMETYQFQPVSAANLRDHLTACSGMDMSDFFDDW
                     VYQPGSLAFDVLSTGGNKGTITCLHIQQYLSHATHYGNNVPLTAYFFDYNFNLFDSVD
                     IVMSGDRQLTTIEVPNPGTFVYTILDYRNKLNDAVTSDEVHFTEPGTYSLDNAMMDIE
                     IT"
BASE COUNT          838 a          644 c          592 g          891 t
ORIGIN      
        1 acttgatacc tgatacttga tacctgatac ttgatacttg atacctgata ccaatcccag
       61 atacaaatat cgtgggtggg tcagtgggtg agtttgaatt ttctcaactc tgtgttcctc
      121 tccgtggcct tagcggttaa atgggtttta aagaatgtgt tggatgataa atgatgattc
      181 acgatacacc cttgttcaat cgcggagttt gcggagttag cggagtcaaa ggatcagtca
      241 ctactcacca ttcacgagtc acgattcacg aaacactcat ccactcaccc actggcccac
      301 tgacccactc acctatctcc cccaaaccga tatatcgata tttccaattt caggatcgcg
      361 gaatgggatg tcccagtagc ctgaatactt tatggataca ggaacacgat atccatcaat
      421 ggtgcccata cgcaccttca tgctcacatc aaaatcaaat agcgaagtga aataacttaa
      481 ttgataattt cgagctacaa tctccttgct gtcaacatca aaccaagtag tgagttcatt
      541 aatcacgaca tctttacctt tttcagcttc cggttttttc ttgatggtga aaacaaaaca
      601 atcaacaccc gttgcaaatt tctcgcgcgc gatggtatag tcgtaatact gttgcatctc
      661 cgggctgaat atttccattt tatcgcctat taatggaatg ccgtttaccg gacgtccagg
      721 gttgaaaata agtgttttga gttgttcttt gcgcaattcc aatttatcgt cgctacccgc
      781 attgttgtct gtttctacga tgttaccaca gatggtatcc cgatacaaaa aaatataact
      841 gaatacaccg gcagtgtaat aatttaattg cttgtgttta tcaaaaaaat caccggtgtt
      901 aacttcaaaa ggaaattgca tccacctgca tttgccatcc aggaattgcg acatcctatt
      961 gctgtaagat gcaatggaaa gatggtcttc gttaaacata cgtacggttg cactgccatc
     1021 atattcaatg cgtcgaaggt tttgaaatgc acgataaaat gtagtatcct cctgtatcat
     1081 ttgcacaaaa tcctgcacat tcaggccatc ccgaacggca tgaacaacaa atgaatcaac
     1141 ggttatgatc tgaacagggc tcgtttcaac ctgagctttc aactgctgta taccaaatac
     1201 caccaggagt aacaccaagc attttgtaac tttacagatc atccgtgtaa aattaacatt
     1261 ttatgcttcg agaaaaatgg ttcctcctga ttttgacggc ttgcatgcag caggcatatg
     1321 ctgctacacc aactatcagc gactcaattg atatacgaca ttacgatatc aatcttgata
     1381 tcacagatta tgcaggtcag actataaatg gttccactac cttacatatc gctgcactgg
     1441 ttgatggcat taccaccctc ccactcgact tgctttcact tgatgttgat ggcattacag
     1501 atggcaatgg caatgcactt tcatttacgt acgatgagcc ttcattgatc atcacattat
     1561 ttacaccgct aaatattggc gattctgcaa atatcatcat ccaatactcc ggtgatcccg
     1621 cacaagatgg ctcttggggt ggctggtact ggagtggtga ttatagttat caattaggtg
     1681 tgggatttga tgcaattccg cataattatg gcagagcctg gttcccatgt ttcgataact
     1741 ttattgaacg ctcaactttt agctatgaca taaaaacaac aaatgataaa aaggcttttt
     1801 gcggtggttt attaatcgac tcggttgata atggtgatgg cactatttcc tggcattggg
     1861 aatgtgttca gcaaattccg agttacctgg cgagcgtggc tgtgagtaaa tatgccacca
     1921 tcaacgatgt ttatgcaggc attgcgggag atatccctat tcagctggca tgcaaggctg
     1981 aggataccac cgacatgaaa aattcattta ttcatttgca tgatgcactt agcactttcg
     2041 aaaatcatta tggtcctttt cgttgggatc gcgttgggta tgcggttgtg ccatttagcg
     2101 gaggcgcgat ggaacatgct atgaatattg cttatcccct ctttgcagtc aacggcacta
     2161 cgctctggga aaatttatat gcacatgaat taagtcacca ttggtggggc gatcttacca
     2221 cctgttcaac tccgcaagaa atgtggatga acgaaggatg ggccgtattt tcagaacatt
     2281 tatttcagga atcattatat ggtgaccaag cctatatgga tgccgtaaaa gccaatcaac
     2341 tcgatgtatt acataatgcc gctccgcgag atggcaataa ttatttcgcg atatccggtg
     2401 ttcctgaatc atatacctat ggcgcaacca cctacaacaa aggcgccagc gttattcata
     2461 cacttagagg ctatatgggt gatgacgcat ttttcagttg cattacaagc tttatggaaa
     2521 catatcaatt ccagcctgtg agtgctgcca acctgcgtga tcatttaaca gcatgcagtg
     2581 gcatggatat gtccgatttt tttgatgact gggtgtatca acccggctca cttgcctttg
     2641 atgtgttgag tacaggtggc aataaaggaa cgattacttg tttacatatt cagcagtatt
     2701 tgtctcatgc aacacattat ggtaataatg ttccgctcac tgcctatttt ttcgattata
     2761 attttaatct gttcgactct gttgatatag taatgtcggg cgacagacaa ttaactacca
     2821 tagaagtccc aaatcccgga acattcgtat atacaatact tgattacagg aataaactaa
     2881 atgatgccgt aacttccgat gaagtgcatt ttaccgaacc cggtacttat tccttagata
     2941 atgccatgat ggatattgaa attac
//
LOCUS       DAOQOU010000262         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_25438_length_2963_cov_6.415750, whole genome shotgun sequence.
ACCESSION   DAOQOU010000262 DAOQOU010000000
VERSION     DAOQOU010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25438_length_2963_cov_6.415750"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLM90_14210"
     CDS             <1..265
                     /locus_tag="PLM90_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90587.1"
                     /translation="INAIHDLSYHASITIYPNPANAIVQISANIDISNTNYFISDVTG
                     KTIISDKLPANASISIENLPEGFYTLTLTNDNMQSSVSFIVAH"
     gene            complement(330..950)
                     /locus_tag="PLM90_14215"
     CDS             complement(330..950)
                     /locus_tag="PLM90_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90588.1"
                     /translation="MLLMNNANSTEVLCISPTGQLGKACIELKLNTFDEACSYIAKLP
                     YRRNFHRDQVTGVLIDGCGTCSTKHRFLAALALEQAQQESNKINAGWKDIQLRIGVFE
                     MQANNTPAIAPVLHAAQLNAIPEAHCYLAINDMRCDFTSDKFTIPFQDTLLYETHVDP
                     INLFLMKDSIHKRILEQWAINHAPDFSIERLWEIREACIGALSKEP"
     gene            complement(934..1812)
                     /locus_tag="PLM90_14220"
     CDS             complement(934..1812)
                     /locus_tag="PLM90_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90589.1"
                     /translation="MQRVTVSLIFCCLLSSTLIAQLPAVDIYYAHISMSASGMQLTGL
                     TNITQRVGYDNQPSFSSDGNTILYTSIRDEKQADIYRYHIDSKLTEQLTNTVESEYSP
                     LLTADEKSFTTVRVEKDSTQRLWQIRIKDGKAKLYVEGVDSVGYYWPVNENIIPCFMV
                     TDTPSLMLVNKKANGGRTIAHNIGRCIKVLPGTYQITYLTKDSEQTFTIRAYNYITRE
                     FSVITEVPSISEDYVWTSDKKLLMPRGNAIWVYDTENPSAGWVKGCDIPTLTGKNIYR
                     MALSQDGKSLAFVADE"
     gene            complement(1862..2299)
                     /locus_tag="PLM90_14225"
     CDS             complement(1862..2299)
                     /locus_tag="PLM90_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90590.1"
                     /translation="MNKLLQMPMVAVILKDNFSYMHLFLIAVLSVSMSTSAAGSATQF
                     ELNQLPGELQEVTYYYANGNRQQTGLLEDGLKTGEWISYSPEGTITARAYYDKGVKTG
                     KWRIYNAEGNLTYKICYENGIRKWAQAFDPAGELTAFSYTAEK"
     gene            2388..2762
                     /locus_tag="PLM90_14230"
     CDS             2388..2762
                     /locus_tag="PLM90_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HMZ90591.1"
                     /translation="MAYELTGKVLEIFPTQEVSATFKKREFVIEKSESSGDRTFTDFI
                     KFQLIQDRCALADNFKIGDEVKVTFNIKGSKWEKEGRTNYFTNLDVWRLERVSGNNQA
                     PASQDTYQEPAPLPEVEDDLPF"
     gene            2771..>2963
                     /locus_tag="PLM90_14235"
     CDS             2771..>2963
                     /locus_tag="PLM90_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90592.1"
                     /translation="MTLPVKLSPHRKKRYLRLFTALRKRKSAADDVLAENAHEAVFAA
                     TNCLDCANCCKTHPPLLEGP"
BASE COUNT          870 a          613 c          568 g          912 t
ORIGIN      
        1 tatcaatgcc attcatgacc tgtcatatca tgcttcgata actatttatc caaatcccgc
       61 gaatgctata gtacaaatca gtgcaaatat tgatatttct aataccaact atttcattag
      121 tgatgtgacc ggtaaaacaa tcatcagcga taaattacct gccaatgcaa gtatttcgat
      181 tgaaaattta cctgaaggtt tttatacgct aaccttaact aatgataata tgcaatcatc
      241 agtaagcttt atagtagcgc attaaaaatt gatgatgtag atagtaggat ttttccatcc
      301 gttttgggta actatcaacg gagtctgcat tatggctcct tactcaaagc gccgatgcac
      361 gcctctctta tttcccataa tctttcgata ctgaaatcgg gagcgtggtt gattgcccat
      421 tgctctaata tccttttatg aatcgaatct ttcatcagaa acaggtttat gggatcaaca
      481 tgagtttcat ataataacgt atcctgaaat ggaattgtaa acttgtcaga agtgaaatca
      541 catcgcatat cgtttattgc taagtaacaa tgtgcttcag gaatagcatt tagctgagct
      601 gcatgcaaaa caggtgcaat tgcaggtgtg ttgttagctt gcatttcaaa aacgcctatt
      661 cgcaattgaa tatctttcca gccggcattt attttatttg attcttgttg tgcctgttcc
      721 aaagccaaag cggcaaggaa tctatgtttt gtgctacacg ttccacatcc gtcaattaac
      781 actccggtta cttgatcacg atgaaaattt cggcgataag gcaattttgc aatatacgaa
      841 caagcctcat cgaaggtgtt taatttcagc tcaatacatg cttttcccaa ttgacctgtt
      901 ggagaaatgc agagcacctc agtagaatta gcattattca tcagcaacaa aggccagcga
      961 ttttccgtcc tgagaaagag ccattcggta tatatttttc cccgtcaatg ttggaatatc
     1021 acatcctttc acccatcctg ccgatggatt ttcagtatca taaacccata tcgcatttcc
     1081 acgtggcatc aataattttt tatcggatgt ccatacataa tcttcgctga ttgaaggcac
     1141 ttctgtaatt acggaaaatt cgcgggtgat ataattgtat gccctgatgg tgaatgtctg
     1201 ttccgaatct tttgtcaggt aggtaatttg atatgttcct ggtaggactt taatgcatct
     1261 gccgatatta tgtgcaattg ttcttccgcc attcgctttt ttatttacaa gcatcagcga
     1321 aggggtgtct gttaccataa aacaaggaat aatgttctca tttaccggcc aataatatcc
     1381 cacactgtca acgccttcaa catacaattt cgccttgccg tctttgatgc gtatttgcca
     1441 caaacgctgc gttgagtcct tttcgactct cactgtagtg aatgattttt catcggctgt
     1501 caacaacgga gaatattcac tttcaacggt attggtaagc tgttcagtaa gtttcgagtc
     1561 aatatgatac cgataaatat ctgcctgttt ttcatctcga atagaagtat ataaaatggt
     1621 gttgccatct gatgaaaatg atggctgatt atcgtaacca acgcgttgtg taatattggt
     1681 aagacctgtt aactgcatac cacttgccga cattgaaata tgcgcataat aaatatcaac
     1741 cgccggcaat tgagcgatta aagtacttga gagtagacag caaaaaatca aggagacagt
     1801 tacacgttgc atatgcaata tttgcaacaa atgtaacaaa cctaaatcga ggaaaccggt
     1861 attatttttc ggcggtatat gaaaacgctg ttagttctcc tgccggatca aaggcctgag
     1921 cccacttacg gatgccgttt tcataacaga tcttgtaagt gagatttccc tcagcattat
     1981 aaatccgcca cttacctgtt tttacaccct tgtcataata tgctctagcc gtaatagtgc
     2041 cttcaggaga ataagagatc cattcgcccg tcttcaagcc gtcttccaat aaacccgttt
     2101 gctgcctgtt gccattagca tagtaataag tcacttcctg caactcaccc ggcagttggt
     2161 tcagttcaaa ttgagttgcc gagccggcag cagaggttga catggacaca gataaaacag
     2221 cgattagaaa caggtgcata tatgaaaaat tatccttcaa aattacagca accatgggca
     2281 tttgcagcaa tttgttcata ttaaatttac attcccgtcg ctttgagata acatacgctt
     2341 aacattgaaa tttacagatt gattatcttt ggagaaattt ttgtgaaatg gcttacgaat
     2401 tgacaggcaa agtgttggag atatttccta cccaagaggt ttcagctact tttaaaaagc
     2461 gcgaatttgt tattgaaaaa tctgaatcga gcggtgatcg cacctttacc gattttatta
     2521 aatttcaatt gattcaggat cgatgtgcat tggccgataa tttcaaaatt ggcgatgaag
     2581 tgaaagtaac gttcaacatc aagggttcta aatgggaaaa agaaggtcgt accaattatt
     2641 tcaccaacct cgatgtatgg cgtttggagc gcgtgagtgg aaacaatcag gctccggcat
     2701 ctcaggatac ttatcaggaa cctgcaccac ttcctgaagt tgaagatgat ctgccatttt
     2761 aattgaccgt atgacacttc cagtgaagct ttcgccacac cggaagaaac gttatctacg
     2821 tttatttact gcgctgcgaa aacgcaaatc tgcagctgat gatgtattgg cagaaaatgc
     2881 ccatgaagca gtatttgctg ctactaactg tcttgattgc gccaattgct gcaaaacgca
     2941 tccacctctg cttgaaggac ctg
//
LOCUS       DAOQOU010000263         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_25554_length_2955_cov_6.293448, whole genome shotgun sequence.
ACCESSION   DAOQOU010000263 DAOQOU010000000
VERSION     DAOQOU010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25554_length_2955_cov_6.293448"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1484)
                     /locus_tag="PLM90_14240"
     CDS             complement(204..1484)
                     /locus_tag="PLM90_14240"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ90593.1"
                     /translation="MQIDRNKAPELQSIDRLDIRNYKSTALSNGTSVTYVGGVEQDLI
                     RVELIFHGGRWYEPSQGVSRATCNLMKKGTSTKSAKEIADSIDFYGAQLEVNAGQDFT
                     SVVIYCLNKHLASILPIAEEIMIDAAFRDEELELFVAKQKQKLRINAQNTDFHANRKF
                     NAVLFGNQHPYGYAMTADTLDALQSDVLRNYHRKHYNPGDARIFLSGNVTDDVLNLVE
                     KHFGHYTATGKIISATDKSIHTASEHKYFIDKDDSVQSSVRIGTLAISKTHPDFPEFN
                     VLNTLFGGYFGSRLMTNIREDKGYTYGIYSSISHYRHASYFSIETEVGNEVCASALEE
                     IYKEMDLLKKVAVDEEELSIVRNYMIGGLLRATDGPFNRINVIKNMMLSDLTPVYFDS
                     LEKAIRNITSERLQELAIKYFDHETMKEVVCGKR"
     gene            complement(1493..2731)
                     /locus_tag="PLM90_14245"
     CDS             complement(1493..2731)
                     /locus_tag="PLM90_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ90594.1"
                     /translation="MIHFQKKQLSNGLTVIVNEDHSTQLCAVNVLYKVGARDEVASMT
                     GFAHLFEHLMFGGSVNAPDFDTELQLAGGTNNAYTSNDLTNYYDVLPVSNIETALWLE
                     SDRMLQLDFSERSLEVQRNVVCEEFKEHYINRPYGDVWHILRELVYEKHPYRWPTIGL
                     ELQHVRDARLSDVEAFFYKYYRPNNAILTISGGITTEKAFSLAEKWFGDIPAGEDFIR
                     TYPTEPVQQQAKFLAVQKDVPVNVIYKAWKMPGRIEQGYYATNMLSDVLGTGEGSRLQ
                     HALKKEKKLFTDIAAFVTGSIDTGMFIISGKLAEGVSWDAAEEGIRIELEKIRSAHIT
                     EQELQRIKNAVEMDITGNNTGVLNKAESLAAAEMLEGADLVNTEMERFLRINTNDMLQ
                     AAQTILREEACSTLHYGNVN"
     gene            complement(2810..2883)
                     /locus_tag="PLM90_14250"
     tRNA            complement(2810..2883)
                     /locus_tag="PLM90_14250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2847..2849),aa:Ile,seq:cat)
BASE COUNT          845 a          675 c          559 g          876 t
ORIGIN      
        1 caggtatcaa gtatcaggta tcaagtatca ggtatcaagt atcaagtatc aggtaaatgc
       61 aaactcaaat cacatactct attcttaagt actcaccaca ctcacgattc acgactcacg
      121 aatcacgact caccacccgc ctactcggcg aggtaacctc acccactcac ccactcacca
      181 cccccctact cggcgaggca agctcacctc ttcccacaaa ccacttcttt catcgtctca
      241 tgatcgaagt atttaatggc gagttcttgc aggcgttcgg atgtgatatt gcgaatggct
      301 ttttcaagac tgtcgaaata aacaggagtg agatcactca acatcatatt tttgattacg
      361 ttgatgcggt tgaaaggacc atcggtagcg cgaagcaatc cgccgatcat ataattgcga
      421 acaatagaaa gttcttcttc gtcgacggca acttttttca acaaatccat ttctttatag
      481 atttcttcca gtgccgaggc gcacacttca ttacctactt ctgtctcaat cgagaaataa
      541 ctcgcatgtc gataatggga aatggatgaa tatataccat aggtatatcc cttatcctca
      601 cgaatatttg tcatcagtcg tgaaccgaaa taaccgccaa ataatgtatt caatacattg
      661 aattccggaa aatcaggatg cgttttagaa atggcaagtg tgcctatgcg tactgatgat
      721 tgaacggaat cgtctttatc aataaagtat ttatgctctg atgcagtgtg aatggattta
      781 tcggtagcag aaataatttt ccctgtagca gtatagtgac caaaatgttt ttccactaaa
      841 tttaatacat catctgtaac attgccggaa agaaaaatgc gcgcatctcc cggattgtaa
      901 tgcttgcgat gataattgcg caacacatca ctttgcaatg catcaagcgt atcggcagtc
      961 atcgcataac catacggatg ctgattgcca aatagtacgg cattaaattt tctgtttgca
     1021 tggaaatcgg tattctgcgc attgatgcgg agtttttgtt tttgcttggc gacaaataat
     1081 tcaagttcct cgtctctgaa ggcagcatcg atcattattt cttctgcaat aggaagtata
     1141 ctggcgaggt gtttgtttaa acagtaaatt acaacagaag tgaaatcctg accggcattc
     1201 acttccagtt gcgcaccata aaaatcgata ctatcggcaa tctcttttgc cgattttgtt
     1261 gaagtgcctt tcttcatcaa attacacgta gcgcgactca caccttgcga aggttcatac
     1321 cacctgccgc cgtgaaaaat aagttctacg cggataaggt cttgttcaac accacctaca
     1381 taagtaacag atgtaccgtt gcttaatgca gtgcttttat aattgcgaat atctaaacga
     1441 tctatcgatt gtaattcggg agctttattt ctatctatct gcatctgatt ccttaattaa
     1501 catttccata atgtaaagtg ctgcatgctt cttcgcgcaa aatagtttgt gcggcttgca
     1561 acatatcgtt ggtgttgatt cgcaggaagc gttccatttc agtattcact aaatcggcac
     1621 cctccagcat ttcagcagca gcgagcgatt ccgctttgtt taatacacct gtattattgc
     1681 cggtgatatc catttcaaca gcatttttta tgcgctgcaa ttcctgttca gtgatatgcg
     1741 cactgcgaat tttttccaat tctatgcgaa taccttcttc ggcagcatcc catgaaacac
     1801 cttcagcaag cttgccggaa ataataaaca tgcccgtatc aatagacccc gtaacgaatg
     1861 cagcgatatc ggtaaataat tttttctctt ttttcaaggc atgctgcaag cgcgagcctt
     1921 cacctgttcc caatacatcg ctcagcatat tagtagcata atatccttgt tcaatgcgtc
     1981 ccggcatttt ccatgctttg taaataacat taacaggaac atctttttgc acggcaagaa
     2041 atttagcttg ttgttgaaca ggttctgtcg gatatgttct gataaaatct tcacctgcag
     2101 gaatatcacc gaaccatttt tctgccaacg aaaatgcttt ttcagttgtg atgccaccgc
     2161 taatggtgag aatggcgttg ttcgggcgat aatatttata aaaaaatgct tccacatcac
     2221 tcaacctagc atcgcgcaca tgttgcaatt ctaaccctat tgttggccag cgatacggat
     2281 gcttctcgta aaccaattca cgtaaaatat gccatacatc gccataaggt cggttgatat
     2341 aatgctcttt aaactcttca caaacaacat tgcgttgcac ttccaatgaa cgttcagaaa
     2401 aatcgagttg caacattcta tcgctttcca gccagagtgc agtttcaata ttgctcaccg
     2461 gaagcacatc ataataattc gtgagatcgt tgctggtata ggcattattt gttccgcctg
     2521 ccaattgcaa ttcagtatcg aaatcaggcg cattcaccga accgccaaac atcaggtgtt
     2581 caaacaagtg agcgaatccg gtcattgatg ccacctcatc gcgggcacct actttatata
     2641 atacgttcac tgcacaaagc tgggtgctat gatcttcatt cacgatcacc gtgagcccat
     2701 tcgacagttg tttcttttga aaatgaatca tggatgcaaa attgggaaat ggaagataaa
     2761 gttcatgtag taccaatgaa aaaggcgcca aaagcgcctt ttcgagtagt gggcccacct
     2821 ggaatcgaac caggcaccta ctgattatga gtcagttgct ctaaccgaat gagctatagg
     2881 cccgaaaatt tgttcgttat tacaataatt tgggagtgca aatataggca ttcgcctatg
     2941 ttttttaaac gtatg
//
LOCUS       DAOQOU010000264         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_25898_length_2933_cov_5.999653, whole genome shotgun sequence.
ACCESSION   DAOQOU010000264 DAOQOU010000000
VERSION     DAOQOU010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25898_length_2933_cov_5.999653"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(586..2091)
                     /locus_tag="PLM90_14255"
     CDS             complement(586..2091)
                     /locus_tag="PLM90_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90595.1"
                     /translation="MRKLIIMVIATALSVSSFAQSDVDALRYSMLDFGGTARSLGSGN
                     AYGALGGDMSVTSMNPAGLAIYRSSELTITPGLLSINSDATYYGQSTTDDRYHFQLNN
                     VGLVFANVNDGKANATDGWVGGAFALTYNKVANYNNAVTYSGFNDGSSLLNTYADFLN
                     SGGGTNPGDVFDKDPFGAGLAWETYLLNPLGTDSTQYYPLITDGNVQQTKVMQTDGGM
                     GEFDISFAGNYGNRLYIGATIGIPHIRYVQSYTWKEEDINNEYNDFNSYSLVDVTETY
                     GTGINAKFGLIYRLNDYLRLGGAVHTPTLFGMSDNYYSSMSSDLNSGDYSYDSPIGEY
                     NYELVTPWRVIGSAAVTLKNIGFISAEYEFVDYSESFFNFNRTLDAGDLSYETTVNTN
                     IDTKYGASSNIRLGAELMYDVFRFRAGYIMSATPFNDGIAAEGADLAKNTYTAGIGIK
                     DKSYFIDLAFAHSNIKQYDIQYVYDNGSGVNDGAVINTSINNFLLSFGFRF"
     gene            complement(2127..>2933)
                     /locus_tag="PLM90_14260"
     CDS             complement(2127..>2933)
                     /locus_tag="PLM90_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90596.1"
                     /translation="ANILSAGYDKAGNGTTDMNHILTDKGSLVVDKGDVNMTAAAAND
                     NNVAGVAELPSIKDKTLSDHSVVAAINEPVALNSSSTGVTEGTTRTDAGVRVIKYYSA
                     DAQNASVQTDRPQPVVSDRGPRGDYGTSVNKPTTTGNNTVTNGVKNNTTSRPEYQQTP
                     SREPVYNNNQTQRPDQPSYDRPSQTKPSYEQPKQPKQPTYEQPRNNTPSRSNDAPSKP
                     SKSYDTPKSNSGSSSSRPSYNSGSSSRPSNSGSSGSKPGGSSPNKRSGGN"
BASE COUNT          793 a          614 c          625 g          901 t
ORIGIN      
        1 aagattaatg ttaattttct ctcattgtaa tccatattca cggaaaaccg ttaatgaatt
       61 ctcaggttca aatagagatt tgaagagccg aaaaatcgaa gggctgtgca agtataaaat
      121 tcacgatata ccagcagcga ggcaatttca tggttcacga tttggtagtg agtagtgggc
      181 ctgcctcgtc gcaggtgggt agtgagtagt gagacaaatg cggtggaatg tgctttgcat
      241 ttactctatg ctactctgtg ttcctctccg tgttctttgt ggttgaatga tcagtggatg
      301 agtgggtcag tgggtgagtt tggattcact ctgtgccact tagtgttcct cttcgtggct
      361 attgtggtta aattggtttt gagggttgcg attgtgaaat tggtatcagg tatcaagtaa
      421 aaaaaaattc acgagtcacg aatcacgagt cacgtaaact cacccacccg cctgctcggc
      481 gaggcaagct cacccaagca tcttctcttc aatctccttt ctcctctctc ctaaaaaaaa
      541 tagccctccc gaaccaccag gagggcctgt cttcactgac aatcattaaa acctaaaacc
      601 aaaacttaac agaaaattgt ttatcgatgt attaatgaca gctccgtcat ttacgccgga
      661 accattgtca tacacatatt gaatatcata ttgcttgata ttgctatgtg cgaaggcgag
      721 gtcaataaaa tatgatttat cttttatacc aataccggca gtataagtat ttttcgcaag
      781 gtctgcacct tcggcagcaa tgccatcatt gaaaggtgta gcactcatga tatatcctgc
      841 gcgaaaacga aatacatcat acattaattc ggcaccgaga cgaatattgc tagatgcacc
      901 atattttgtg tcgatattcg tgtttactgt tgtttcataa ctcaagtcgc cggcatctag
      961 tgtgcgattg aaattgaaga atgattcact gtaatcaaca aattcatatt cagcagaaat
     1021 aaagccgatg tttttcagtg taaccgctgc actaccgatc acgcgccatg gtgttacgag
     1081 ttcgtagtta tattcaccaa ttggagaatc atatgaataa tcacctgaat tgaggtcgct
     1141 cgacatgctt gaataataat tatccgacat accaaacaaa gttggtgtat gcacagcacc
     1201 gcctaatcgc agataatcgt ttaagcgata gataagtccg aatttagcat tgatacctgt
     1261 tccgtaggtt tcagtgacat caacaagcga ataagaattg aaatcattgt attcgttatt
     1321 gatatcttct tccttccagg tgtagctttg cacgtagcgg atatgtggta ttcctatggt
     1381 tgctccgata tacaatctat tgccataatt tcctgcaaac gaaatatcaa attcacccat
     1441 gccaccatca gtctgcatca cttttgtttg ctgcacattg ccgtcggtga tcaatggata
     1501 atattgtgtt gaatcggttc ccagtggatt caataaatat gtttcccaag ccaaaccggc
     1561 tccaaacgga tctttatcga aaacatctcc cggatttgtg cctccgccgc tatttagaaa
     1621 atcggcatat gtatttaaca atgaactgcc atcattaaat ccgctgtaag taacagcatt
     1681 gttataattc gcaactttat tataagtaag tgcaaatgca ccacctaccc agccatcagt
     1741 agcatttgct tttccatcat tcacatttgc gaatacaagt ccgacgttat tcaattgaaa
     1801 atgatagcga tcatcggtgg ttgattgtcc ataatacgta gcatcagaat taatgctgag
     1861 caatcccggt gtgatggtca gttctgagct gcgatatata gcgagacctg cagggttcat
     1921 ggaggtaaca ctcatgtcgc cacccagcgc accgtatgca tttcctgatc cgagcgatct
     1981 tgctgttcca ccgaaatcga gcatggaata gcgaagagca tctacatcac tttgtgcaaa
     2041 ggaacttaca gacaaagcag tagctataac cattataatt agcttgcgca tggttagttt
     2101 attttagagt tgattaaaaa tgcagatcaa ttacctccgg aacgtttatt cggagaactt
     2161 ccacccggct ttgaacctga tgatccactg ttgctggggc gactgctgct tccgctgtta
     2221 tatgacggac gtgaagagct gctaccggaa ttgctttttg gtgtatcata gctttttgaa
     2281 ggcttagatg gcgcatcatt tgagcgtgaa ggcgtattgt tgcgcggttg ctcatatgtt
     2341 ggctgctttg gctgcttcgg ttgttcgtag ctaggtttag tttgtgacgg acgatcgtat
     2401 gatggttgat caggacgttg tgtctgatta ttattataaa ccggctcacg tgaaggtgtt
     2461 tgctgatatt caggacgtga agtggtatta tttttcactc cgttagttac agtattatta
     2521 cctgttgtgg ttggtttgtt tacacttgtt ccataatcac cgcgtggacc tctatctgaa
     2581 actacaggtt gtgggcgatc agtttgcaca cttgcatttt gcgcatcagc actgtaatat
     2641 ttaattacgc gaacacctgc atcagtgcga gttgttcctt cagtgacacc tgttgatgag
     2701 ctatttaatg caacgggttc attgatggca gcaacaacag aatgatcaga taatgttttg
     2761 tctttgattg acggcaactc tgcaacacct gcaacgttat tatcatttgc tgcagcggca
     2821 gtcatgttca catcaccttt atcaaccacc agcgatccct tatcggtaag gatatggttc
     2881 atatcagtag taccattgcc ggctttatcg taacctgcgg aaaggatgtt tgc
//
LOCUS       DAOQOU010000265         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_26009_length_2927_cov_5.331128, whole genome shotgun sequence.
ACCESSION   DAOQOU010000265 DAOQOU010000000
VERSION     DAOQOU010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26009_length_2927_cov_5.331128"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(522..2720)
                     /locus_tag="PLM90_14265"
     CDS             complement(522..2720)
                     /locus_tag="PLM90_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HMZ90597.1"
                     /translation="MSTNRTAAMAELLHRELVPIELPASKISEFFGSNVFSDAEMRKH
                     LSEEAYNSIRHSIENGQKIDRKIADQVAASMKAWAMGKGATHYTHWFQPLTGATAEKH
                     DSFFTAVATAVGVETFSGSALVQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAFIMD
                     SVSGKTLCIPTIFVSYTGEALDYKAPLLKSLHLLEEAAVPVCQYFDKNVNRVNATLGW
                     EQEYFLVDEALFLARPDLSICGRSLVGHYPAKGQQLEDHYFGTIPERIYNYMVDFETE
                     AHKLGIPLRTRHNEVAPGQYECAPLFEEASIAVDHNLLLMDVMEKVARRHKLRVLFHE
                     KPFAGVNGSGKHNNWSMGTDTGKNLLSPGKTPRTNLQFLTFFINTIKAVHEYADLLRV
                     SIAAAGNDHRLGANEAPPAIISAFIGTALREVLNEIENRVTEKDFDESDKTDLRLDIH
                     NKIPDLLLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSKAMTTLNTIVATQLIAFRTE
                     VDALIKGGENKDSAILKVLRRYITESKNILFEGDNYSEEWAKEATEKRGLSNVKTTPE
                     AIEFYGTDKAKKVFVSNNILSDRELHARQEIEFEEYAKKIQIEARIYGELVYNYVLPA
                     SITYQNELLDNILKLKAVGLNEAHYKEQLDLAQTITLHINHLRSSLERMVEERKRANH
                     IEHAREKAYAYCNDVKPLFDTIREHADMLERYLPDASWPLPKYRELLFVK"
BASE COUNT          734 a          616 c          739 g          838 t
ORIGIN      
        1 agttgtaagc cgatggattg gttgaaagag aaacagcgct caagctgtat tttagcgcaa
       61 tagttattta cgggagcgat ggtgcttacg tcaatgccga cagcggtgta ttgagcgaag
      121 gcaatgttga aggtgatgag ggccagggta agggttacaa ggtttttcat agtattgatt
      181 ttcagcaaaa gtatccctgc ccaccggctt ccattacgca tttttcgcag gtggcctcca
      241 aagaattacg gattttcgca aatgaaacgc ttataagcgg ctatggggtt tattttattg
      301 aaaataaacc ccataggatt gctttttttc tcacaaaggc gcaaaggttt tcacgcagaa
      361 tacgcagagg gaaaagcaaa ctcaccactc acgattcacc cttgttcaat cgcggagttt
      421 gcggagttag cggagtttgt ggattagtca cgattcacga gtctcgattc acgaaacact
      481 cacccacccg cctatcggcg aggcaagctc acccactgac ctcacttcac gaacaacaac
      541 tcacgatact tcggcaatgg ccatgaggca tcggggaggt agcgttcgag catgtcggcg
      601 tgctcgcgga tggtgtcgaa gagtggtttc acgtcattgc aataagcata tgctttttcg
      661 cgagcgtgct cgatatggtt ggcacgtttg cgttcttcta ccatgcgttc gagtgaagaa
      721 cgcaggtgat tgatatgcag cgtgatggtt tgtgcgaggt cgagctgttc tttgtaatgg
      781 gcttcgttca atcctactgc ttttaatttg aggatattgt cgagcaactc attttgataa
      841 gtgatggaag cgggtaatac atagttgtat accaattcgc cataaatacg cgcttcgatt
      901 tgaattttct tggcgtattc ttcaaattca atttcctgac gtgcatgtaa ttcgcgatcg
      961 ctgaggatat tgttggaaac gaaaactttt ttcgctttgt cggtgccata gaattcgata
     1021 gcctcaggtg tggttttaac attgctgagt ccacgttttt ctgtcgcctc ttttgcccat
     1081 tcttcgctgt aattatcacc ttcgaataaa atgtttttac tttccgtgat gtagcgacgc
     1141 aatactttca atatcgcgct gtctttattc tcaccacctt ttatcaaggc atccacttca
     1201 gtgcggaagg caattaattg cgtagcaacg atagtgttca acgtggtcat cgccttagag
     1261 cagttagcac ttgaacctac tgcacggaat tcgaatttat taccggtgaa tgcaaatgga
     1321 cttgtgcggt tgcgatctgt attgtcgagc agtaggtccg gaattttatt atggatatct
     1381 aagcgaagat ctgttttatc cgactcgtcg aagtcttttt cagtaacgcg attttcaatt
     1441 tcattcaata cttcgcgcaa ggctgttccg atgaatgcag aaataattgc cggaggtgct
     1501 tcgttggcac caaggcgatg atcgtttcct gcggctgcga tagaaacgcg gagcagatcg
     1561 gcatactcat gaacggcctt gatcgtattg ataaagaagg tgagaaattg aagattggtg
     1621 cgtggtgttt ttcccggact cagcaaattc ttgccggtat cggtgcccat cgaccagtta
     1681 ttgtgttttc ctgagccgtt gacacctgca aatggttttt cgtggaataa tacacgcaat
     1741 ttatggcgac gcgctacttt ttccatcaca tccattaata acaagttatg atcaacggca
     1801 atggatgctt cttcaaataa tggcgcacat tcgtattgtc cgggagccac ctcattgtga
     1861 cgcgtgcgca gtgggatacc gagtttgtgt gcttcggttt cgaaatccac catataatta
     1921 tatatacgct ccggaatagt tccgaagtaa tgatcttcca actgctgacc tttagcaggg
     1981 tagtggccca ccaatgaacg accgcaaatg ctcaaatcgg gacgggcgag gaacagtgct
     2041 tcatctacca aaaaatattc ttgctcccat ccgagggtgg catttacgcg gttaacgttt
     2101 ttgtcgaagt attggcaaac cggaacggca gcttcttcga gcagatgtaa tgatttaaga
     2161 agtggtgctt tataatcgag cgcttcaccg gtataagaaa cgaagattgt tggaatacag
     2221 agtgtcttgc cactcacaga atccatgatg aacgccggtg atgatggatc ccatgcggta
     2281 taaccacgcg cttcgaaagt actgcggata ccaccgcttg ggaagctgga ggcatcaggt
     2341 tcttgttgca ccaatgcact tccgctgaat gtctctacac caacagctgt ggctactgcc
     2401 gtaaaaaagc tgtcgtgctt ttcggcagtg gctcctgtaa gcggttggaa ccagtgggta
     2461 taatgagtgg cgcctttgcc catagcccag gctttcatgc tggctgccac ctgatctgca
     2521 atcttgcggt cgattttttg tccgttctct atcgaatggc gaatgctgtt atacgcttcc
     2581 tcgctgaggt gtttgcgcat ttcggcgtcg ctgaacacgt tagagccaaa gaattcagag
     2641 atttttgagg caggaagttc gattggcacc agttcgcggt gcaacagttc tgccatggca
     2701 gcggtacggt tagtagacat tttgtggtgg attttgaggc aaagttagaa cctccttttt
     2761 aaaaaacatg aaaattttgg cacagggatg tggaaaagtg attattcggt taaaaaataa
     2821 catatattga aaaacataaa ttgattggcg aaggagagag gaaaaaggag agaggagagg
     2881 gtgagtgggt gagcttgcct cgccgagcag gcgggtgggt gagctta
//
LOCUS       DAOQOU010000266         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_27820_length_2821_cov_4.788503, whole genome shotgun sequence.
ACCESSION   DAOQOU010000266 DAOQOU010000000
VERSION     DAOQOU010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27820_length_2821_cov_4.788503"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLM90_14270"
     CDS             complement(<1..535)
                     /locus_tag="PLM90_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90598.1"
                     /translation="MKRVLIITYYWPPAGGVAVQRWVKMARYLRQFGWEPVIYTAENG
                     EMPVIDESLAATLPAGLEVIKRPIWEPYSWYKRFLGLSKEEKINAAFLQEKKKTGLLQ
                     HLAIWVRGNLFIPDARRFWIKPSVRFLTDYVKSHPVDAIISTGPPHSMHLIAMGLKER
                     TGIPWIADFRDPWTRIDY"
     gene            638..>2821
                     /locus_tag="PLM90_14275"
     CDS             638..>2821
                     /locus_tag="PLM90_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90599.1"
                     /translation="MQKNNGSKKLLTYLAVFVFFLAFSALYFYPLLQGKILFQSDIMN
                     FVGVSKEAVDHYDTTGDAALWTNSMFGGMPTYQVSLPENGNIFERINIYLGAFLPRPA
                     NYMFIALVTFFLLMRSFRVNTWLSISGALCYALGTYLITFMEAGHNTKVNALALMPLV
                     FTGLQYLWQRKWLLGSALTLFAMTCEITANHPQINYYMFLIIAIWGISELVFAIREKT
                     VLQFVKITAVIIAVTLMAVAANTSKLWTTYEYSKESIRGGSQLTSQKTVQDDGLNRDY
                     AFGWSSGVAESFSIMYPNFAGGGSGFSFLQTRDGETIDGPMLPFIQQLYQKDPKKTQE
                     ILNYSGQAGKYWGDMPFTSGPIYVGSILCFLFILGALLADNRMRWWLIAATVLSLFLG
                     WGKNFEAFNYWMFDHFPLYNKFRTVSMAMTMLCFTIPVLAIYIVNKFLSDNNEITHEK
                     KYWALKWASLATGALSLILMMSGSLFDLLKPVEEEYLMQANSIEASTFFEMLKDARAQ
                     MIRTDVLTTTFIIALAAGALWLYLRKTITAKIAIAIICILPVLDLMIVDSKYLGKDNY
                     QEANFYDQRLRSSLPVIKDKDPYYRVWNTMADPDKDGTTSYLYKSIGGYHGAKLQRYQ
                     DIIDGYLHKGDVHVLSMLNTKYVIGQRQGKITFDRNPAALGNVWFVDSVRMVKTADEE
                     FNGLANFSPQNTVIVNEEFKNLIPATTIQRDSTASIKLKAYGMDEI"
BASE COUNT          794 a          599 c          599 g          829 t
ORIGIN      
        1 aatagtcaat ccgcgtccaa gggtctctaa aatcggcgat ccatggaata cctgttcgct
       61 cctttaagcc catagctatc agatgcatgc tatgtggcgg cccggttgaa ataatggcat
      121 cgacaggatg cgatttaaca tagtcagtca gaaatcgaac agagggtttt atccaaaagc
      181 gtcgggcgtc aggaatgaaa aggttacctc tcacccatat tgccagatgc tgcaaaagcc
      241 ctgttttttt cttttcctgc aggaaggctg cattgatctt ttcttctttt gaaagcccca
      301 aaaagcgctt gtaccacgaa tatggctccc aaattggtcg cttgatcacc tccagtcccg
      361 caggaagtgt agccgccaac gactcgtcaa taacaggcat ttcgccattt tcagcggtat
      421 aaatgacggg ttcccaacca aattgtcgca gatatcgtgc cattttcacc cagcgctgca
      481 cagcaacacc gcctgccgga ggccaataat aggtgataat gagaacgcgc ttcaaggtgg
      541 ttggtttcag ggctaaagtt aaaacaactt ccacaaccct actttccggc ctttagctgt
      601 aacttcttaa ctttaacgcc gcaaataaaa gcctatcatg caaaaaaaca acggctctaa
      661 gaaactgctt acctatctcg cagtttttgt tttcttcctc gcattcagcg cattgtattt
      721 ttatccgcta ttgcaaggta aaattctgtt tcagagtgat atcatgaact ttgtcggcgt
      781 aagtaaagaa gccgttgatc attatgacac taccggcgat gctgcacttt ggaccaacag
      841 catgtttggc ggtatgccta cttatcaggt gtcattacct gaaaatggaa atatttttga
      901 gagaataaat atttatctcg gcgcatttct tcctcgccct gccaattata tgttcatcgc
      961 gctggtcaca ttcttcctgc tgatgcgcag ttttagagtg aacacatggt tgagtatttc
     1021 cggtgcactc tgctatgcgc tgggaacgta tctgattacc ttcatggagg ccggccataa
     1081 tactaaagta aatgcactgg cattgatgcc gctggtattt acaggattgc aatatctatg
     1141 gcaacggaaa tggttattgg gaagcgcatt gacattattt gccatgacat gcgagatcac
     1201 tgcaaaccac cctcaaatta attattacat gttcctcatt attgccatat ggggaatttc
     1261 agaattagta tttgcaatcc gtgaaaaaac agttttacag tttgtaaaaa taacagcagt
     1321 gatcattgct gttacattaa tggcagttgc agccaatact tcaaaattgt ggaccacata
     1381 cgagtattct aaagaatcca tccgcggagg tagtcagctt accagtcaga aaaccgttca
     1441 ggatgatgga cttaatcgcg attatgcttt tggatggagc tctggtgttg cggaatcatt
     1501 cagcattatg tatccgaatt ttgccggtgg cggatcaggt ttctccttcc tccaaacccg
     1561 cgatggcgaa actattgacg ggccaatgct gccatttatt caacagctct atcaaaagga
     1621 tccgaagaaa actcaagaaa tattaaacta ttccggacaa gcaggaaaat attggggcga
     1681 catgccattc acgagcggac caatatatgt aggttcaatt ttatgcttct tgtttattct
     1741 tggtgcattg cttgccgata acagaatgcg ctggtggttg attgcagcta cggtattgtc
     1801 gctgttcctt gggtggggta aaaatttcga agcatttaat tattggatgt tcgatcattt
     1861 cccgctctac aataaattca gaacagtatc tatggcaatg accatgctct gctttacgat
     1921 tcctgtgctt gccatttata ttgtcaataa attcctgagt gataacaatg agattacaca
     1981 tgagaaaaaa tattgggcgt tgaaatgggc ttctcttgct acaggtgctt tatcattgat
     2041 attgatgatg tcgggaagtt tgtttgattt attaaagcct gttgaagaag aatatttaat
     2101 gcaagccaat agcatagaag catccacatt cttcgaaatg ctgaaggatg cacgcgctca
     2161 gatgattcgc accgatgttc tgactacaac attcatcatt gctcttgcag caggtgcact
     2221 ttggctttat ctgcgcaaaa ccattactgc taaaattgcc atcgccatca tttgcatttt
     2281 gccggtgctc gatttgatga ttgttgattc taaatatttg ggcaaagaca attatcagga
     2341 agctaatttt tatgatcaac gactgcgttc ttcgttgccg gtgatcaagg ataaagatcc
     2401 ttattaccgc gtatggaata ccatggcaga tcctgacaaa gatggcacta catcttatct
     2461 gtataaaagc attggcggct accacggagc gaaattgcaa cgctatcaag atattattga
     2521 tggctatttg cataaaggcg atgtgcatgt attgagtatg ctcaatacca aatatgtgat
     2581 tgggcagcgt caaggcaaaa tcaccttcga tcgcaatcct gccgctttgg gaaatgtttg
     2641 gtttgtagat tctgtgcgca tggtaaaaac tgctgatgaa gaatttaacg gcttagccaa
     2701 cttcagtcct caaaataccg taattgtaaa tgaagaattt aaaaatctga ttcccgcaac
     2761 caccatacaa cgcgatagca ccgcaagtat taagcttaaa gcttatggta tggatgagat
     2821 c
//
LOCUS       DAOQOU010000267         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_28008_length_2811_cov_5.548258, whole genome shotgun sequence.
ACCESSION   DAOQOU010000267 DAOQOU010000000
VERSION     DAOQOU010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28008_length_2811_cov_5.548258"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..2687
                     /locus_tag="PLM90_14280"
     CDS             81..2687
                     /locus_tag="PLM90_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ90600.1"
                     /translation="MIKKLYVLAIGLLMAVGLQAQTVIKGNISAQDGGALPSATVMEK
                     GTNNGTVADLDGNFSITLIKTPATLVFTFVGYTTQEIEVSAQASLRVVMESGSYSLSG
                     VEVVGTRSLNRSATESMSPIDIIDVTAVTGANGQLDLNQLLQYTAPSFNSNRQSGSDG
                     ADHIDPATLRGLGPDQTLVLVNGKRYHQSSLINIFGTRGRGNTGTDLNTIPAAAIERI
                     EILRDGASAQYGSDAIAGVINIVLKDNVNEFTGNLNTGAYKAEYNFNEGSLDGQNLNL
                     NGNYGFKIGDDGFFNVTADYNYRGHTNRAEFIGPDFPALTDYRNQFGDAAVTNFTSWF
                     NMRIGLSNGASFYAFGGRNSRLVDAYAYTRSITGDNAHPERVVLDIYPEGYFDPIIAS
                     VVADNSLSAGIRGDMRGWDVDFNNTIGNNNMHYYGRNTLNASLGAASPTEFDDGGFSL
                     MQNTTSLDFSRYYASCLKGMNVAFGAEYRIENYNIFAGEEGSWYNYGGMYITETDTVE
                     RPGGSQGFPGFQPGDVTDEYRSNIGAYIDLEADFTDAFAMSAALRYEDYSDFGNTVTG
                     KIAARLAMGKNYALRGSFSTGFRAPSLPQIYFSSTYTNVESGVIVDEVIADNKSTIAR
                     ELGIPALKEETSVNGTIGITAKPMPALNITLDAYYVTIADRIVLTDGFGTDDDVIGGV
                     LEDLNVGYARFFTNAVDTKTTGLDAIISYKIYMKENTLTFTYAGNFNKMEVTAIHTND
                     LLAGKEDNYFSDREEYFLLASAPPVKMHLNAEYATKKFNANLRFNYFGEISLINYSGN
                     LYTYTPKYTIDASVGYDLTGNLHVTVGGANLTDQYPDPTDPFETETGGAWDAVQMGFN
                     GMFLFAKLGFTF"
BASE COUNT          801 a          617 c          606 g          787 t
ORIGIN      
        1 acgctccaca cctgccatgt gcaaatcgtt tttacagcta tatcacaaaa ccttagatta
       61 gcttcacaaa acccaatttt atgatcaaaa agctttacgt attggccatc ggcttgctga
      121 tggctgtagg attacaggca caaactgtca ttaaggggaa tatttccgca caagacggag
      181 gagcacttcc ttcggcaaca gtgatggaaa aaggaaccaa taacgggact gtggctgatc
      241 tcgatggaaa cttctccatc accttaatta agacacctgc aacactggtg ttcacttttg
      301 tgggctatac tacgcaggaa attgaagtat cggcccaggc atcacttcgt gtagtgatgg
      361 aatccggctc atacagtctc agtggggtag aggttgttgg tacacgcagc cttaatcgct
      421 ccgcaactga aagcatgtcg cccatcgata ttattgacgt gactgccgta acgggtgcaa
      481 acggacaact cgacctcaac caattattgc aatacacagc accgtcattc aactcgaacc
      541 gtcaatccgg ttctgatggt gccgaccata tcgaccctgc aacattgcgc ggtcttggtc
      601 cggaccagac actcgttttg gtgaacggaa aacgctatca ccaatcatca ctgatcaata
      661 ttttcggaac gcgtggtcgt ggtaatacag gtactgattt aaatactatt cctgcagccg
      721 caatcgaacg cattgaaatt ttgcgcgatg gtgcttctgc acaatatggt tctgatgcta
      781 tcgccggcgt aatcaatatt gtattgaaag ataatgtgaa tgaattcaca ggaaatttaa
      841 ataccggtgc atataaggct gaatataatt tcaacgaagg atcactcgat ggtcagaatt
      901 taaatctgaa tggtaactac ggatttaaaa ttggcgatga tggatttttc aatgtaacag
      961 cagattataa ttatcgcgga cataccaacc gcgcagaatt tattggacct gattttcctg
     1021 cacttactga ttatcgcaat cagttcggtg atgcagcggt aaccaatttc acttcctggt
     1081 tcaacatgcg catcggtctt agcaacggtg cttcgtttta tgcatttggt ggaagaaata
     1141 gtcgcctcgt agatgcatat gcttatacgc gttctatcac aggagataat gcacatcctg
     1201 aacgcgtggt attagatatt tatcctgaag gatattttga tcctattatc gcatcagtag
     1261 ttgctgataa ttcattatcg gccggtattc gtggtgatat gcgtggatgg gatgtagatt
     1321 ttaataatac catcggtaat aataatatgc attattatgg tcgcaatacc ttgaatgcat
     1381 cattgggtgc agcatcgcca acagaatttg atgatggtgg tttcagtctg atgcaaaata
     1441 ctaccagcct cgatttcagc cgttattatg cctcttgcct caaaggtatg aacgtggcat
     1501 tcggtgctga atatcgcatt gaaaactata acatatttgc cggtgaagaa ggctcatggt
     1561 ataattatgg tggtatgtat atcaccgaaa cagatactgt tgaacgtcct ggtggttctc
     1621 agggattccc tggtttccag cccggcgatg taacagatga atatcgcagt aatatcggtg
     1681 cttatatcga ccttgaagca gacttcacag atgcatttgc catgagcgct gcattgcgtt
     1741 atgaagatta cagtgacttc ggaaataccg taacaggaaa aattgctgcg cgtttggcca
     1801 tgggaaaaaa ttatgcactc cgtggttcat tcagcacagg cttccgtgca ccatcattac
     1861 cacaaattta tttcagctca acatatacca acgtagaatc aggtgttatt gttgatgaag
     1921 ttattgctga taataaaagc actatcgcgc gcgaattagg tatccctgca ttgaaagagg
     1981 agaccagcgt gaacggtacg ataggtatca ctgcaaaacc aatgcctgca ttgaatatta
     2041 cattagatgc ctattatgta accattgccg atcgtattgt attgaccgat ggtttcggta
     2101 ctgatgatga tgtaattggc ggagtattgg aagatctgaa tgtaggttat gcccgtttct
     2161 ttacaaacgc ggtagatacc aaaacgacag gtcttgatgc gatcatcagc tataaaattt
     2221 atatgaagga aaatacactc accttcacat atgccggcaa cttcaataag atggaagtaa
     2281 ctgccatcca taccaatgat ttgcttgcag gtaaagagga taattatttc agcgatcgcg
     2341 aagaatattt cttgctcgcc agtgcccctc cggtgaagat gcacctgaat gcagaatatg
     2401 ccactaaaaa attcaatgct aatctgcgat ttaattattt cggtgagata tcactcatca
     2461 actacagcgg caacttgtac acctatacac caaaatacac catcgacgcc agcgtaggtt
     2521 acgaccttac cggcaatctg cacgtaactg tgggtggtgc caaccttacc gatcaatatc
     2581 ctgatcctac cgatccgttc gaaaccgaga ccggtggcgc ctgggatgct gttcaaatgg
     2641 gcttcaacgg tatgttcctg ttcgcaaaac tcggatttac cttctaagcg acgtccattg
     2701 cctaatgata acaaaacggc tccccttgcg gagccgtttt tatttccccc caaacactct
     2761 gcgtattcta aggtacccat ttcaccaaat ttatttcagg ctgccactaa a
//
LOCUS       DAOQOU010000268         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_28009_length_2811_cov_5.277576, whole genome shotgun sequence.
ACCESSION   DAOQOU010000268 DAOQOU010000000
VERSION     DAOQOU010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28009_length_2811_cov_5.277576"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>2811)
                     /locus_tag="PLM90_14285"
     CDS             complement(147..>2811)
                     /locus_tag="PLM90_14285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMZ90601.1"
                     /translation="LEHMMFKGTTSFGTNNWDREKMVIEGISQLYEKHRRETDPEKKK
                     AIYAEIDKLSQEAAKFAIPNEYDKMITSLGAKGTNAYTSKEQTVYVNDIPANELEKWL
                     VIESERFRTCVLRLFHTELEAVYEEYNMGQDRDGSKVYETYYRALFPTTPYGTQTTIG
                     EGEHLKNPSMVNIMGYFYTYYVPNNMAVCLSGDLDYDKTIALIDKYFGAYVNKQVGVR
                     EFNTEKPITEVQRREVYGQESAYVQLGYRVPGANTEDNIIAKLIAGMLYNQQAGLLDL
                     DLNQQQKVLDAAAYHTEWRDYSVLTLAADPRDGQTLEECEQLLQAELEKIRKGEFDEW
                     LMKAVINDYQLSRTKSFESNSGRAGAFVNTFIRGIDWGAYLNEYNVMSTITKADIVEF
                     ANTYLGKNSYVVVYKRKGEDKNIKKVEKPVITPIEPNRDMSSKFLTSFMELPSSRVAP
                     EFVDYSTKISNTMLNNDVPMAYIKNEENQLFELYYILDMGSYNDRELALAINYLPYLG
                     TDKYSPAQLQQEFFKYGLSFTVNAGSDRVYVSLSGLESNLEKGIELFEHLLSHVQPDN
                     KAYREYVDGIIKEREDAKTNKNYIFGVAMLNYAKYGAVSPQTDILSEQQLRNVLPATL
                     TDKIKSLTGYKHRVFYYGQMRPELVQTMLNKYHVTKAPLMDYPPATKYPEKETNENKV
                     LFVHYPMVQAQISFISKDENFDKTVLPQARMFNEYFGSGLSSIVFQEIRETKALAYSA
                     YANYSTPLNKDEAHYVRASVSTQADKMQAAMEAMLEIMNNMPQAEEQFNASLESVLKQ
                     IESERIIRSNIFWNYESMKRKGLNYDYRRDVYMAARGMSMQDMQEFFNNHISDRHYTI
                     IVMGDRTKVDLNYLKSIGAFREMTMEEIFGY"
BASE COUNT          772 a          611 c          517 g          911 t
ORIGIN      
        1 agaaacaaat tgattagccg ctgaaggctg gtaagggctt cgaaggaggg aatggggaaa
       61 tagccccgga aataaccctt gcaaaaccta aaacctccga ataccaatgc ataaaacatt
      121 gctattcgga ggccaggtag tggatatcaa tatccaaaaa tctcttccat agtcatctca
      181 cggaaagcac ctatcgactt gagatagttc aggtcaactt tggtgcggtc gcccataaca
      241 atgatagtat agtgacggtc gctgatatga ttattgaaaa attcctgcat atcctgcatg
      301 ctcattcctc gcgctgccat gtaaacatcg cgacgataat cgtaattcag tcctttgcgc
      361 ttcatgcttt cataattcca gaaaatattg gagcggataa ttctttcact ttctatttgc
      421 ttaagcacgg attccaaact cgcattgaat tgttcttccg cctgaggcat attattcatg
      481 atttcaagca tggcttccat cgcggcttgc atcttatctg cttgcgtact caccgatgca
      541 cgaacataat gtgcctcatc tttattcagt ggtgtagaat aatttgcata ggctgaatat
      601 gccaatgctt ttgtttcacg tatttcctgg aaaacaatac ttgacaaacc gctaccaaaa
      661 tattcgttaa acatacgcgc ctgaggcaac acggttttat cgaaattttc gtccttcgaa
      721 ataaatgaga tttgcgcctg caccatagga taatgcacaa atagcacctt attttcattg
      781 gtttcctttt ccggatattt agtagccggc ggataatcca tgagcggtgc ttttgttaca
      841 tggtatttat tcaacattgt ttgcacgagc tccggacgca tctgaccata ataaaacacg
      901 cgatgtttgt atccggtgag cgatttgatt ttatcagtca atgttgcggg caacacattt
      961 cgcaattgtt gttcgctgag tatatcagtt tgtggcgaaa cggcaccata ctttgcataa
     1021 ttgagcatcg ccacgccgaa aatgtagttc ttatttgttt tagcatcctc acgttccttg
     1081 atgataccgt ctacatattc acgataagcc ttgttatccg gttgcacatg acttagcaag
     1141 tgttcaaata attcaattcc cttttcgagg tttgattcaa gtcctgaaag cgaaacataa
     1201 actctatcac ttccggcatt tactgtaaat gaaagtccgt acttgaagaa ctcttgctgc
     1261 aattgcgcag ggctatattt atcggtaccc aaataaggca agtaattaat cgccaatgcc
     1321 agttcgcgat cgttatatga ccccatatcg agaatgtagt ataattcgaa gagttgattt
     1381 tcctcgtttt taatatacgc catcggcaca tcattattaa gcatggtgtt gcttatttta
     1441 gtgctgtaat caacgaattc cggagctacg cgactacttg gtaattccat aaacgatgta
     1501 aggaatttac ttgacatatc gcgattgggc tctatcggtg taattacagg tttttctacc
     1561 tttttaatat tcttgtcttc acctttgcgc ttatacacta ctacataact attttttccc
     1621 aaataggtat tggcaaattc aacaatatct gctttggtga ttgttgacat gacattgtat
     1681 tcattcaagt atgcacccca atcaattcca cgaataaatg tattcacaaa tgcacctgcg
     1741 cgacctgaat ttgattcgaa tgatttggtg cgtgataatt gatagtcgtt gatcacggct
     1801 ttcatcaacc attcatcaaa ctcacccttt ctgatctttt cgagttctgc ctgcaataat
     1861 tgttcacact cttctagagt ctgaccatca cgcgggtctg ctgccagggt aagaacacta
     1921 taatcgcgcc attcggtatg ataggctgct gcatccagta ctttttgttg ctgattcaag
     1981 tctaagtcga gcaaaccggc ctgctgatta tacaacatac ctgcaatcaa tttcgcaata
     2041 atattatcct ctgtatttgc acccggaacg cgatatccta attgcacata tgccgattct
     2101 tgtccataca cttcgcgacg ttgcacttca gtaataggtt tttcggtatt aaactcgcga
     2161 acaccaactt gtttatttac ataagcaccg aaatatttat caatgagtgc aattgttttg
     2221 tcataatcaa gatcaccact taaacaaaca gccatattat ttggcacata ataagtatag
     2281 aagtaaccca tgatattcac catagaagga ttcttcaaat gttctccttc accaatggta
     2341 gtttgtgtgc cataaggtgt tgttgggaat agcgcgcggt aataagtttc atacactttg
     2401 ctgccatcgc gatcttgtcc catattgtat tcctcataca ctgcttctaa ttcagtatga
     2461 aataaacgca acacacaagt gcgaaagcgc tcactttcaa tcaccaacca tttttcaagt
     2521 tcattggccg gaatatcatt aacataaaca gtttgttctt tgctggtata tgcatttgta
     2581 ccttttgcac caagtgaggt gatcattttg tcatactcgt tggggatggc gaattttgct
     2641 gcttcttgac ttagcttatc aatttcagca tagattgctt ttttcttttc aggatctgtt
     2701 tcacggcgat gtttttcata taattgagaa ataccttcaa tcaccatttt ttcacgatcc
     2761 cagttattgg ttccaaagct ggtagtgcct ttgaacatca tatgttccag g
//
LOCUS       DAOQOU010000269         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_28543_length_2781_cov_4.778430, whole genome shotgun sequence.
ACCESSION   DAOQOU010000269 DAOQOU010000000
VERSION     DAOQOU010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28543_length_2781_cov_4.778430"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..936)
                     /locus_tag="PLM90_14290"
     CDS             complement(340..936)
                     /locus_tag="PLM90_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90602.1"
                     /translation="MNIRDIYKAPDTKVFIITESDYDNELNWVIEKTDHELIPEEENY
                     YLVKAFEVTRAEIKDCYLGVSIPERFADIVVKLNARGEAVVEYISQQANSIIPAIAHD
                     AAGYYELYYAKENPQAGIDVLRSGLAIAKNKSAIAEDLGYLLIDEDRTEEAIEAFTIS
                     ETCGPTTRFTFYELAKLYGKIGQVEKQGEYLEKYKAFQ"
     gene            1127..2626
                     /locus_tag="PLM90_14295"
     CDS             1127..2626
                     /locus_tag="PLM90_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90603.1"
                     /translation="MKESLFVRKSSLILFFSLLMLPAFGQMIRYKDYDDVFIFGDYSY
                     FTESDASEYSDLVYLHEGYAVINHTDIQKTGRRYIYANGKQVVSGGYVQYAGWEKCAD
                     NSCWGDAYFVKDGNLYVICKAALKTGNLFHSFNAAGEEIDLPLPMAIDTAFDEKMIGK
                     NGTDERIKYYTKYLPLQDSTKGIIYFKPYHSNLSPKTTNLPCFLYDFVTGEVKAINVV
                     SFAGNVAVNRRPDQDYFLFKYYIENDYYYAYYDYTHNTGGYLTDLDGEDVTAFYFKDG
                     NYYCETEKKLYKIESGKAVRMKEINVSLNTSDMLVYDAPVKSNFNLDYKWDKSTYGQK
                     NKQYPQLVKNGVVIEELPFLVQGYAKGLDKIVLFADVSQTSYYFLDSVAKANGNPSME
                     LIGEYQAAEDLLTEKYNELVALNKKVDESGVAYETCYNKGTEDCASQKLGLKFAYENY
                     YAKHEELSSALEKHKDKLDPASYTQMRNEIDNNLAAMRANALIQYVMGW"
BASE COUNT          802 a          494 c          647 g          838 t
ORIGIN      
        1 ccatgcccta attaacaagc attacaaagc tttttaaaca aatatttgcc aatttaccat
       61 gtcacaaaat tgcccttacg aaatgcagac gtttaacctc ctcctagccc cgattgcagc
      121 ggaaaccccg agcaaatgat gtgatgatgg gttgatgagg tgatgggctg atggaatgca
      181 tggtttttag gctgaggtaa agcagcgagg agttgcagcg gaaagcaggt gaggattgcg
      241 ctgcggaacg gttttatcat gctccaaata taaaagaacc tacctaaaac cctgtttcag
      301 gcagtcctga ggctgaaatc gttgaatggg gtggttatgc tattggaacg ccttgtattt
      361 ttccagatac tcaccctgct tctccacttg cccaatcttt ccataaagct tagccaactc
      421 atagaaagta aaccgggtgg tgggaccgca ggtttcgctg atggtgaagg cttcgatggc
      481 ttcttcggtg cggtcttcgt cgatcagcaa atagccgagg tcttcggcaa tggcgctttt
      541 gtttttggca atggctaatc cgcttcttaa tacatctatg cctgcttgcg gattttcttt
      601 tgcgtaatat aactcgtagt aaccggcggc atcatgggca atggcgggaa tgatggagtt
      661 tgcttgttgc gatatgtatt ctactaccgc ctctcccctc gcatttagct tgactacaat
      721 atcggcaaat cgctcgggta ttgatacacc caggtagcaa tctttaattt cggcccgggt
      781 aacttcaaat gcttttacga ggtaataatt ttcttcttcg ggtatcaact catgatcggt
      841 tttttctatt acccaattca gctcattgtc gtaatcgctt tctgttataa tgaacacttt
      901 ggtgtcgggt gctttgtata tatctcttat gttcatgcgc ggtgaaatca gattacgctt
      961 gccattgctt gccacaaatg tagctgagaa actttaattt tcagatgcgt gcaacataac
     1021 acttgcaatt gtttgtcgga ggtgcaattg atgttgccat gcagtcggat agatttctat
     1081 tttttagtga aacgatttat ttttgtttgc caatataatc atgctgatga aagaatcctt
     1141 gtttgtgcgg aaatcgagct taatcttgtt ttttagtttg ctgatgctgc ctgctttcgg
     1201 gcaaatgatt cggtataagg attatgatga tgtatttatt tttggtgatt attcctactt
     1261 caccgaaagc gatgcaagtg aatattcgga tcttgtttat ctgcatgaag ggtatgcggt
     1321 cataaatcat acggatattc aaaaaaccgg caggagatac atttacgcta acggcaaaca
     1381 agttgttagc ggtggatacg ttcaatatgc aggttgggaa aaatgtgctg acaactcttg
     1441 ttggggtgat gcctattttg ttaaggatgg aaatctatat gtgatatgca aagcagcatt
     1501 gaaaacgggt aatctgttcc atagctttaa tgccgccggt gaggagattg atctcccatt
     1561 gccgatggct attgacactg cgtttgatga aaagatgata ggcaagaatg gcactgacga
     1621 aagaataaag tattacacaa aatatctgcc cttacaggac tccacaaagg gtattattta
     1681 tttcaagcca tatcattcaa atctgagtcc taaaacaacg aatttgcctt gttttttgta
     1741 tgattttgtt acgggtgaag tgaaagctat taatgtggtg agttttgcgg gcaatgttgc
     1801 ggtgaacagg cgtcctgatc aggattattt tttattcaaa tattacatcg agaacgatta
     1861 ttattatgcc tattacgatt atacgcataa caccggaggt tatttaactg atttagatgg
     1921 ggaagatgta accgcattct acttcaagga tggtaactac tattgtgaaa cggagaagaa
     1981 gctgtataaa attgagtccg gtaaggctgt tcgcatgaag gagataaacg tatcgctaaa
     2041 tacatcggac atgctagtgt atgatgcacc tgtgaaaagt aatttcaatc tggattataa
     2101 atgggataaa agcacttatg ggcagaagaa taagcagtat ccgcaattgg ttaaaaatgg
     2161 cgtggtaatt gaagagcttc catttctggt gcagggttat gcgaagggat tggataaaat
     2221 agtgctgttt gctgatgtat ctcaaacttc ctattatttt ttagactcgg tggcaaaagc
     2281 aaatggaaat ccatcgatgg aactcattgg cgaatatcag gctgcagaag atctgctgac
     2341 tgaaaaatac aatgagttgg tggcactgaa taaaaaagtg gatgaaagtg gtgtggcata
     2401 cgagacttgt tataacaagg gaactgaaga ttgtgcttca cagaaattag gtttgaagtt
     2461 tgcctatgaa aattattacg cgaaacacga agaactttct tctgcactcg aaaagcacaa
     2521 agacaaatta gatcctgcat cgtacacaca aatgcgtaat gaaattgata ataatttagc
     2581 tgctatgcgc gcaaatgcat tgatacaata tgtaatgggg tggtaggaat tagattacaa
     2641 agggaataac atgtaacaaa cgaacatact cttttggggt tcgcgcaaag gcgctaagag
     2701 gggaaggcgc aaagtctgcc actccaaaca caaccattgt ctgaatcacg gattattggg
     2761 attaaaggat tgcacggatt g
//
LOCUS       DAOQOU010000270         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_29462_length_2733_cov_6.409634, whole genome shotgun sequence.
ACCESSION   DAOQOU010000270 DAOQOU010000000
VERSION     DAOQOU010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29462_length_2733_cov_6.409634"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /locus_tag="PLM90_14300"
     CDS             complement(<1..2134)
                     /locus_tag="PLM90_14300"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ90604.1"
                     /translation="MITLLRRSLLLAIAIFALNTAQAQVNLLTQGFESLPFPPTGWSN
                     VRMTGPTLPGTWARYGNGLFPVQTPHTGSWQIRYNSHNYLAGTSGELRTAVLDFTTSG
                     TYTVSFWMYRDNWVAADKLEVYVNTTQTSVGGTLLGTINRDRNQSPVVGTNGWYQYTF
                     TIPGAFNTSTNYIVFKATSGFGNDIYVDDISVDRLAPAAPGCVTTFSPATGTTGACIN
                     QTISWGIVALASGYKLTMGSNAPNYNNVANNVDLGVALSYSTFLNPSTTYNWKITPYN
                     AFGDATGCGFNSFTTGSGTCYCTPVYLDGSCGSEDFIDDFSTSAGTTNISNNNSGCTT
                     NPSNYTYFSGMTVTATQGNSFNVSMQSGPTYAEGFAIWADWNQDGDFADAGEYAFNSG
                     VATLAVVNGSITVPYTATVGTTRMRVRCAYNYVPASDTYCTTFSEGETEDYNITVNPC
                     PATTYYADADGDGFGNAASTTLSCTGAPSGYVANNTDCNDSNASIKPGGTEVCNALDD
                     DCDASIDEGLTFTTYYQDLDGDGYGTNAATTSSCAGSAPSGYAAANTDCNDANASIKP
                     GATEICNGIDDDCDALIDDGVTFYTYYFDADGDGYGTAATTTSSCAVVPPSGYVVLGT
                     DCNDANAAIKPSALEICNAIDDDCDASIDEGLPVFTYYRDADLDTYGNNAITTSTCAA
                     APTGYVSNNLDCNDANAAIKPGATEICNS"
     gene            complement(2270..>2733)
                     /locus_tag="PLM90_14305"
     CDS             complement(2270..>2733)
                     /locus_tag="PLM90_14305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90605.1"
                     /translation="KASYDATYTWQWYKDGSPIVGATSYLYIATLPGSYNCQITNAYG
                     CTRTTAALTVTACREGETVDAVVEETFELYPNPTESIFTLDMELNTTEQVASVYILNI
                     VGEQVYAGTVGIDNGTINTTITLGNDIAAGMYVVKVIVAEKEYTKQLIIQK"
BASE COUNT          777 a          579 c          563 g          814 t
ORIGIN      
        1 tgctattaca aatttctgta gcgccgggtt taattgctgc attcgcatcg ttgcaatcta
       61 aattattaga aacataaccg gttggagcag ccgcgcaagt tgatgtagtg atggcattat
      121 ttccataagt atctaaatca gcatcgcgat aataagtaaa caccggtaag ccttcatcta
      181 tcgatgcatc gcaatcatca tcaatagcat tacaaatttc aagtgcactt ggtttaattg
      241 ccgcattggc atcattacaa tctgttccaa gcacaacata tcctgaagga ggcactacag
      301 cacatgaact ggtagttgtt gcggcagtac catatccgtc gccatcggca tcgaaataat
      361 atgtgtagaa tgttacgcca tcatcaatca gcgcgtcaca atcatcatca atgccattac
      421 agatttctgt tgctcccggt ttaattgagg cattcgcatc attacaatcg gtatttgcag
      481 cggcataacc ggaaggagct gaacctgcgc aagaagaagt agttgcagca tttgttccgt
      541 aaccatcgcc atcaagatcc tgataataag ttgtgaatgt aagtccttca tctattgaag
      601 catcacaatc atcgtcaagt gcattacaaa cttctgttcc acccggttta attgaggcat
      661 tcgagtcatt gcaatcagta ttatttgcaa cataacctga aggcgctcct gtgcaactta
      721 atgttgttga agcagcattt ccaaatccat cgccatcagc atcagcataa tatgttgtag
      781 ccgggcatgg atttaccgtg atattataat cttctgtttc tccttcactg aaagtggtgc
      841 aataggtatc cgatgcgggc acataattgt aagcgcatct gacacgcatg cgtgttgtgc
      901 ctactgttgc agtataggga actgtgattg atccgtttac aacagctaat gttgctacgc
      961 cggaattaaa agcatattca cccgcatcag caaaatcgcc atcctgattc caatcggccc
     1021 aaatggcaaa gccctctgca tatgttggtc cgctttgcat actcacatta aatgaatttc
     1081 cctgagtagc ggttacggtc atgcccgaga aataagtgta attacttgga ttagtagtac
     1141 atccactatt attatttgaa atattcgttg tacctgctga agttgaaaaa tcatctataa
     1201 aatcttccga gccgcatgat ccatcgagat aaacaggcgt gcaataacaa gttccggaac
     1261 ctgttgtgaa gctgttaaaa ccacaacctg ttgcatcacc aaatgcattg tatggtgtaa
     1321 ttttccaatt atatgttgtt gatggattaa ggaatgtact ataactcaat gcaacaccaa
     1381 gatcaacatt gttggcaacg ttattataat taggtgcatt gcttcccatg gttaatttat
     1441 atcctgaagc aagtgcaact attccccacg aaattgtttg attaatacat gctccggtag
     1501 taccagtagc aggtgaaaat gtagtaacac atccgggggc agccggcgct aaacggtcaa
     1561 ctgaaatatc atcaacatat atatcatttc cgaaaccgga tgtggcttta aatacaatat
     1621 aatttgttga agtattaaat gctccgggaa ttgtaaaagt atattgatac caaccattag
     1681 ttcccacgac aggcgattga tttctatcgc gattgatagt tcccaaaagt gtaccgccta
     1741 cagaagtttg agtggtattt acatatactt ccaatttatc tgctgctacc cagttatcgc
     1801 gatacatcca aaaactaacc gtataagttc ctgaagtagt aaagtcgaga acagctgtgc
     1861 gcaattcacc ggatgttcca gccaggtaat tgtgtgagtt atagcgtatt tgccatgaac
     1921 ccgtatgcgg tgtttgtacc gggaataagc catttccata tcttgcccat gttccgggca
     1981 atgtaggacc ggtcattctc acatttgacc aacctgttgg ggggaatggc agagattcaa
     2041 atccttgtgt aagtaagttt acctgtgcct gagcagtgtt taacgcaaaa attgctattg
     2101 ccagcaataa gctgcgtctt agtaatgtaa tcatagcctt gttgagtatg ggttaaagat
     2161 agtgggtagg tttcgcttcc aattactaaa ataaggatat tccaatttcg cgacaaaaaa
     2221 atcccgcagg agttgacctg cgggattgta aatgattgtt tgagaatcct tacttctgga
     2281 ttatcagttg tttagtgtat tctttttcag ccactatcac tttcacaaca tacataccgg
     2341 cagcgatatc gttgccaagg gtgatggttg tgttgatggt gccgttgtct ataccaacag
     2401 ttccggcata tacctgctca ccaacgatgt tcaggatata tactgaagct acttgttcag
     2461 tggtattcag ctccatatcg agtgtgaaga tgctttcagt tgggttagga tacagttcaa
     2521 atgtttcctc aaccacagcg tcgactgttt caccttcgcg gcaagcagtt acagttaatg
     2581 ctgctgttgt acgagtacaa ccataggcat ttgtaatctg acagttatag cttcccggta
     2641 atgtagcgat atacaagtag cttgtagctc caacaattgg gctgccatct ttataccatt
     2701 gccatgtgta agtagcgtcg tagctggctt tca
//
LOCUS       DAOQOU010000271         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_29695_length_2721_cov_5.009002, whole genome shotgun sequence.
ACCESSION   DAOQOU010000271 DAOQOU010000000
VERSION     DAOQOU010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29695_length_2721_cov_5.009002"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1451
                     /locus_tag="PLM90_14310"
     CDS             114..1451
                     /locus_tag="PLM90_14310"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ90606.1"
                     /translation="MHPTIIKNARIVNEGEILEGDVLIHKGRIEKIGGIVQVSAASTE
                     IDATGKFLMPGIIDDQVHFREPGLTHKADLFSESRACVAGGVTSFMDMPNVNPPSLTQ
                     ELLAQRYALASTKSVANYSFYMGVSNDNADEVLRTDPATVCGIKIFMGSSTGNMLVDD
                     AATLERIFSQAPVLIATHCEDEATIIHNLEAAKATYGEDIPVHMHPIIRNEEACYRSS
                     SFAVSLAKKHNTRLHILHISTAEELALFTNEIPLEQKRITAEACVHHMYFDSNDYAQL
                     GARIKWNPAVKGPRHKPAIFDAVVNDVIDVIATDHAPHTLEEKANKYVKCPGGAPMVQ
                     HSLQVMLSFYFDGKISLEKIAKKMSHDVATCFNIKDRGFIREGYFADMFLLDTDKSIV
                     VSKNNIIAKCGWSPLEGRTMRGVITDTFVNGDHVFSNGMVNDGVRGMRLAFNR"
     gene            complement(1525..>2721)
                     /gene="yidC"
                     /locus_tag="PLM90_14315"
     CDS             complement(1525..>2721)
                     /gene="yidC"
                     /locus_tag="PLM90_14315"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HMZ90607.1"
                     /translation="WRNEMQEQEKNIKYERQYSKLYFGTEDGDVDYKNTNGDLKFEDR
                     IKWVSCQQQFFNATLIAKESFEKNGKIDIFADDTSRFVKRCNTELYLTFNGKDSYEFP
                     MQWYLAPNQYDELKALDLGIETIIPTGSGFIGWINKTAIMPLFHWLSGMISNYGLIIL
                     LMTLILKLALSPLTYRSYLSMAKMRVLNPEVAELREKYGDDQAKLGAEQMKLFRKAGV
                     NPLGGCIPTLLSMPILIAMFRLFPGTIQLRQQSFLWADDMSTYDDIIHWGTNIPFIGT
                     HISIFCILMTISSILYMRMNMQNTSQLTGPMKTVQYITPVFFFFFLNSSPAALTYYYF
                     VSNLTTFGQQWAIRKFLINDEEIHRRIQENKSKPEKAKSGFAKRLEDAMKQSQQIREQ
                     QKKGKK"
BASE COUNT          816 a          531 c          579 g          795 t
ORIGIN      
        1 gtctctgcgc ctctgcgtga aacaaacaca caccacaaac acgtaccacc aattacctga
       61 ttacatggta tatttgtgaa caggcaacag catgaaacgt gttgcaatca aatatgcatc
      121 caacaatcat aaaaaatgca cgcatcgtca atgaaggaga aattcttgaa ggcgatgttc
      181 tcatacataa aggcagaata gaaaaaatcg gtggtattgt acaagtcagt gctgcatcaa
      241 ccgaaataga tgcaacaggg aaattcctga tgcccggtat tattgacgac caagtgcatt
      301 ttcgcgagcc cggactcacg cataaagctg atctcttttc cgaaagcaga gcttgtgttg
      361 cgggtggtgt aacctcattt atggatatgc ccaatgtgaa tcctccgagt ttgacacagg
      421 aattgcttgc acagcgctac gcgttggcat cgacaaaaag tgttgccaat tattcatttt
      481 acatgggtgt gagcaacgac aatgccgatg aagtattgag aaccgatccg gcaactgttt
      541 gcggaataaa aatcttcatg ggttcttcaa caggtaacat gttggttgat gacgctgcta
      601 cactagagcg cattttttct caggctcctg tgttaatcgc aacacattgt gaagacgaag
      661 caaccattat acacaatttg gaagctgcca aagctactta tggagaagat attccggtgc
      721 atatgcatcc gattatccgc aatgaagaag cctgttatcg ttcatcttca tttgctgttt
      781 cacttgctaa aaaacacaat acgagattac atatattgca tatcagtact gcagaagaac
      841 ttgccttatt taccaacgaa atccctcttg aacagaaaag aattaccgct gaagcatgtg
      901 tgcatcatat gtatttcgac agcaatgatt atgcgcaatt gggagcacgc atcaagtgga
      961 atcctgcggt aaaaggtcca agacataagc ctgcaatttt tgatgccgta gtgaatgatg
     1021 tcatcgatgt gattgccaca gaccatgcac cccacaccct tgaagagaaa gcaaataagt
     1081 atgtgaaatg cccgggagga gcgcccatgg tgcaacatag tttgcaggtg atgttgagtt
     1141 tttatttcga tgggaaaatt tctctggaaa aaatcgcgaa gaaaatgagt cacgatgttg
     1201 ccacctgttt taatattaag gacagaggat ttattcgcga aggatatttt gctgatatgt
     1261 ttttacttga tactgataaa agcattgttg tatcgaaaaa taatatcatt gccaaatgcg
     1321 gctggagccc gctggaggga cgtactatgc gcggtgtgat aacagataca ttcgtaaatg
     1381 gcgatcatgt attcagcaat ggtatggtga acgacggtgt tcgcggcatg cgattggctt
     1441 ttaatagatg aggatgacga taaaccaaaa ttaaaatggc gacttgataa tttcaaatcg
     1501 ccatttttta tgttaatgta gtaattactt tttacctttt ttctgttgct cacgaatttg
     1561 ttgcgattgt ttcatggcat cttcgagacg ttttgcaaat ccggattttg ctttttcagg
     1621 ttttgatttg ttttcctgaa tgcgacgatg aatttcttca tcattgatca ggaattttct
     1681 gattgcccat tgctgtccga aagtggtaag gttacttacg aagtaataat atgttaaggc
     1741 tgcaggtgaa ctattcagga agaagaaaaa gaaaactggt gtgatatatt gcaccgtttt
     1801 catcgggcct gtaagttgag aagtattctg catattcatg cgcatgtaca gaatgctgct
     1861 gatagtcatc agaatacaga aaatactgat atgtgttcca atgaatggaa tattagtgcc
     1921 ccaatgaatg atatcatcgt aagtcgacat gtcatcagcc cacaagaatg attgttgacg
     1981 caattgtatt gttccgggga ataagcggaa catcgcgatg agaattggca tcgacagcag
     2041 ggtagggata caacctccta atggattcac acctgctttg cggaacaatt tcatttgttc
     2101 agcacctaat ttagcctgat catcaccata tttttcgcgt aattcggcaa cttcaggatt
     2161 gagcacacgc atcttcgcca tagataaata agagcggtag gtaagcggcg acaatgccag
     2221 tttcagaatc aaggtcatca acaaaatgat cagtccatag ttgctgatca taccgctcag
     2281 ccaatggaac aaaggcataa tagctgtttt gttaatccaa ccgataaatc ctgaacccgt
     2341 tggaataata gtttcaatac cgagatcaag tgcttttaac tcgtcgtatt gatttggggc
     2401 aagataccat tgcatgggaa actcatagga atctttgcca ttaaatgtga ggtataattc
     2461 ggtattacaa cgcttcacaa atcgagaagt atcatcggca aaaatgtcga tcttgccatt
     2521 tttctcaaag ctctcttttg caataagagt ggcattaaaa aattgttgct gacagctcac
     2581 ccatttaatg cgatcttcaa atttgagatc gccattggtg tttttgtagt caacatcgcc
     2641 atcttctgtt ccgaaatata atttagaata ctgacgctcg tatttgatat ttttttcctg
     2701 ctcctgcatt tcatttctcc a
//
LOCUS       DAOQOU010000272         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_32348_length_2590_cov_4.527416, whole genome shotgun sequence.
ACCESSION   DAOQOU010000272 DAOQOU010000000
VERSION     DAOQOU010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32348_length_2590_cov_4.527416"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..2025
                     /gene="glgP"
                     /locus_tag="PLM90_14320"
     CDS             313..2025
                     /gene="glgP"
                     /locus_tag="PLM90_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HMZ90608.1"
                     /translation="MINYSNWQLPYEPNPAFKQRTAYFCMEFGVHQALKIYSGGLGYL
                     AGSHMRSAYELRQNLFGIGILWRYGYYDQVRDDHGFMAVQFQKKLYTYLKQTDIAFTM
                     PINGKDVMVKVYYLAPEVFNSAPLFLLSTDFPENDEEVRQYTHRLYDRDHEIRIAQNM
                     ILGIGGAKVVELLGGGDIYHMNEGHALAMTFYLFSKWKDVDEVRKHVVFTTHTPEKAG
                     NEEHNINDMERMGFFSGVPLDIIRHITKTEGDMLGYTPSALYMSKIANGVSKLHGEVS
                     REMWRDYPGICQITHITNAQQKKFWADRELNQAYINRDDMGLRVIKKELKRELFEVVA
                     NQTGKIFDPNVFTIVWSRRFAEYKRANLLMRNKERFLNFLNNPKYPVQVVWAGKPYPF
                     DHGAINTFNDIISFTMDRPNLAVLTGYEIELSYLLKRGADVWLNTPRRPREASGTSGM
                     TAAMNGAVNVSIMDGWIPEFAHHGENCFILPPADHYTMENVDIDNFDYENLMSVLENE
                     VVPMYYDNPEKWLNIVKKSMHDVYPYFDSDRMAVEYYQKVYQHPFSAVDKLLDSVFDN
                     NNNG"
     gene            complement(2028..2285)
                     /locus_tag="PLM90_14325"
     CDS             complement(2028..2285)
                     /locus_tag="PLM90_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90609.1"
                     /translation="MRIVGYIEHPVLKISVFSWSEKYIVKIEAGLFEQSYKFRQADFE
                     QWEDLKAFFDADMISDIMTTFKKMSADASASAKRLSSSGKA"
     gene            complement(2282..>2590)
                     /locus_tag="PLM90_14330"
     CDS             complement(2282..>2590)
                     /locus_tag="PLM90_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ90610.1"
                     /translation="LLEGPDIKRIAKHLHLSEASFMSNYLLLDEDGDWVFHTVPCPFL
                     ESDNKCRIYDHRPKACREYPHTNRKKLYQISDLTLTNAEICPAVAPILDKLAAELHIP
                     "
BASE COUNT          722 a          585 c          600 g          683 t
ORIGIN      
        1 taatagggaa tgcaattata ttgctttgca atattttaat tccgaagcgc aatcagctcc
       61 ctctctatcg acggtaggag atggagagct tgccgctccg acgaaaggag gtgtggggcg
      121 gggggtgagg cgggtttcgc gcaaagacag cgaagaggca aagccgcaaa gaaattcacc
      181 actcaccact caccattcac gattcacgat tcacgattca cgagtcacga ttcacgattc
      241 acgaatccac attagctcaa attgcaaaat ctacgttttc tatatattcg cacctgacaa
      301 cgagaacgaa cgatgattaa ctacagcaat tggcaactgc cttatgaacc aaatccggcc
      361 ttcaagcaac gaactgctta tttctgcatg gaatttggtg ttcaccaggc actcaaaata
      421 tacagcggcg gccttggcta ccttgcggga tcgcacatgc gcagtgcata tgaattgcgc
      481 cagaacctct tcggaatagg tattttgtgg cgatatggtt actatgatca ggtgcgcgat
      541 gatcatggat ttatggctgt tcagttccag aagaagcttt acacctacct taaacagact
      601 gatattgcct tcaccatgcc tatcaatggc aaggatgtga tggtgaaagt gtattacctg
      661 gcacctgaag tattcaattc agcaccgctt ttcttactta gcaccgattt ccctgaaaac
      721 gacgaggaag tgcgtcagta tactcaccgc ctctacgacc gcgatcacga gattcgcatt
      781 gctcagaaca tgattctggg tatcggcggc gccaaagtag ttgaattgct tggtggtgga
      841 gacatctacc atatgaatga aggacacgcg ctggcaatga ctttctatct gttcagtaag
      901 tggaaggatg ttgatgaagt gcgtaagcat gttgttttca ccacacatac ccctgaaaag
      961 gccggaaatg aggagcataa tatcaacgat atggagcgca tgggattttt cagcggtgta
     1021 cctcttgata tcatccgcca tattacgaaa acggaaggcg acatgctcgg atatacgcca
     1081 tcagccctct atatgagcaa aattgctaac ggcgtgagca aactccacgg cgaagtaagt
     1141 cgcgaaatgt ggcgcgatta tcccggtatc tgtcagatta cgcatatcac caatgcacag
     1201 cagaagaaat tctgggccga ccgtgaactc aatcaggcgt atatcaatcg cgatgacatg
     1261 ggactgcgcg ttatcaaaaa agagcttaaa cgcgaactct tcgaggttgt tgccaaccaa
     1321 accggcaaaa tcttcgaccc gaatgtgttt actatcgttt ggtcgcgccg attcgccgaa
     1381 tacaaacgtg caaacctgtt gatgcgcaac aaagaacgct ttctcaattt cctcaataat
     1441 ccgaaatatc ccgttcaagt ggtttgggca ggaaagcctt atcccttcga tcacggtgcc
     1501 atcaacacct ttaatgatat catctcattt accatggatc gccctaacct ggcggtgcta
     1561 accggttatg aaatagagtt gagttatctg ttgaaacgcg gtgctgatgt gtggttgaat
     1621 acacctcgtc gcccaaggga agcttctgga acatccggca tgacagcagc aatgaacgga
     1681 gcagtcaacg tgagcatcat ggatggctgg attccggaat ttgctcatca tggtgaaaat
     1741 tgctttatcc tgccgcctgc cgaccattac acaatggaaa acgtagacat cgacaatttc
     1801 gattatgaga acctgatgtc ggtgctggag aatgaggttg tgccaatgta ttacgataac
     1861 cctgaaaaat ggttgaatat cgttaagaag agcatgcacg atgtatatcc ttatttcgat
     1921 agcgatcgca tggcggttga atattatcaa aaggtatatc agcacccttt cagcgccgta
     1981 gataagctac tcgattcagt tttcgataat aataataacg gctaggatca ggctttaccg
     2041 ctgctgctaa ggcgttttgc agatgcggaa gcatcggccg acattttctt gaaggtggtc
     2101 atgatgtctg atatcatatc ggcgtcgaag aatgctttaa gatcttccca ttgttcgaaa
     2161 tcggcctgtc ggaatttata ggactgttcg aataagccgg cttcgatctt tacgatatac
     2221 ttctcactcc agctaaatac gctgattttc aataccgggt gttcaatgta gccaactatc
     2281 ctcatggaat atgtaattct gctgccaatt tatccaaaat cggggcaact gcagggcaga
     2341 tttctgcatt tgttaaggtc agatcactga tttgatacaa tttctttctg ttggtatgcg
     2401 ggtattcgcg acaagctttc gggcgatggt cgtaaattcg acatttatta tctgactcca
     2461 gaaaagggca ggggacagta tgaaataccc agtcgccgtc ctcatccagc aacaaataat
     2521 tactcataaa cgatgcctct gatagatgta aatgtttggc aatgcgttta atatcaggtc
     2581 cttcaagcag
//
LOCUS       DAOQOU010000273         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_33320_length_2549_cov_4.153168, whole genome shotgun sequence.
ACCESSION   DAOQOU010000273 DAOQOU010000000
VERSION     DAOQOU010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33320_length_2549_cov_4.153168"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLM90_14335"
     CDS             complement(<1..471)
                     /locus_tag="PLM90_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ90611.1"
                     /translation="MKIENGQQAPDFLMYDTDKQPVRLSALKGENVLLLFFPFAFTST
                     CTAELCATRDDIAFYNNMNAKVFGISVDAPYSLKVFREEQQLNFALLSDFNKTVSETY
                     GCIYDRWGMELKGVSKRSAFIIDKEGIVRYAEILENAGELPNFEAIRSTLEQLNA"
     gene            786..1700
                     /locus_tag="PLM90_14340"
     CDS             786..1700
                     /locus_tag="PLM90_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90612.1"
                     /translation="MKIRLLLTVAALGVATMGFSQVPNGNMDSWDTYTSLASGLSCDF
                     PTQWDVPDKIAADLGIADRNCTKETANVHGGAAAARLETKTLNVLGTPLDVPGTITTG
                     IIGFDFVTFTPSVEGGASVSTNYTALTGYYQYAPSGSDTMNVIVLMMSGADTIGLGQY
                     RDAATSTSYQMFDCPITYFGLGTADKMQITITSSGGFTSLVPGSVLFVDDLAVTGGAD
                     VEDWNGYGIAHNIYPNPASDYINILNPATNDVTIEIYSLTGQKVGEQTLAPDMNSINL
                     ENYTSGIYTFRLVDNGTVVYSSKFVVKK"
     gene            complement(1777..2208)
                     /locus_tag="PLM90_14345"
     CDS             complement(1777..2208)
                     /locus_tag="PLM90_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ90613.1"
                     /translation="MTMLTVKRRPSGIPTMFNMFDDLFNVPSTNFRGSFVPSVNVEQH
                     DKGYHLEVAAPGFDKGDFDVHVEKDVLRITGKKETKDEVKEKNYTRKEFSFGSFERSF
                     TLPENIDGEQIAASYSNGILHIELPLKTAVQVENKKQIEIK"
     gene            complement(2360..>2549)
                     /locus_tag="PLM90_14350"
     CDS             complement(2360..>2549)
                     /locus_tag="PLM90_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HMZ90614.1"
                     /translation="CGHSHILKVMRDQTTGTLCMNPGASGVHGFHKLRTMIRFQASNG
                     QILQPEVIELGPRGRITK"
BASE COUNT          691 a          549 c          530 g          779 t
ORIGIN      
        1 ggcattcaat tgctcgagcg ttgagcgtat tgcttcgaaa tttggcagct ctcccgcatt
       61 ttcaagtatt tctgcatacc gtactatacc ttctttgtcg atgatgaatg cgcttcgttt
      121 ggaaacacct ttcaattcca ttccccaacg atcgtagata cagccatatg tttcagaaac
      181 ggttttgttg aagtcgctaa gcaaggcgaa atttaattgt tgctcctcgc gaaacacttt
      241 taaactataa ggtgcatcca cgctgatgcc gaatactttc gcgttcatgt tattgtaaaa
      301 tgcaatgtca tcgcgtgtag cacataactc agcagtgcaa gtactggtaa atgcaaacgg
      361 gaaaaacaga agtaatacat tttcaccttt tagggccgac aatcgcacgg gttgcttgtc
      421 agtgtcatac attaaaaaat ccggggcttg ttgtccgttc tcgatcttca tctgtgttaa
      481 aaatttaaag taaagatatg ctaaacaggg gctataacat ctttacatga aaagggttca
      541 ataaagtgca tggaactgtg cgatgcgata cctgcctgaa gtatatgccc tttgccatgc
      601 cgaagccgac agataaaggc ttgatttcca tggcagtagg ggattgtgcg gaggctattc
      661 gatgtggtct tttgggcgtc aaagaagctt tttgaccgat ttgtgaaaga aatatctcca
      721 caggttaggg aaatcctgat tatatttatc tttgaaacac aattcaaata actaaaaact
      781 tacgtatgaa gattcgttta cttttaactg ttgccgctct cggcgtagct accatgggtt
      841 tcagccaggt gccaaatggc aatatggact cttgggatac ctatacttct ctagcttcag
      901 gactatcttg cgacttcccg acacaatggg atgtgccgga taagattgct gccgatcttg
      961 gtatcgccga tcgcaactgt accaaagaaa ctgcaaacgt gcatggcggc gctgctgcag
     1021 ctcgtttgga gacaaaaaca ttgaacgtat tgggtactcc attggatgtt cccggaacta
     1081 tcacaaccgg tatcatcggt tttgatttcg ttacgtttac gccttcagtt gaaggtggtg
     1141 cttcagtttc tactaattac acagctctta caggttacta tcaatatgcc ccatcaggca
     1201 gcgataccat gaatgttatc gtgttgatga tgagtggtgc tgataccatc ggtttgggtc
     1261 aatatcgcga tgctgcaacg tcaacttctt atcagatgtt cgattgccct atcacttact
     1321 tcggacttgg aactgccgat aaaatgcaaa tcaccatcac ttcatcaggt ggtttcactt
     1381 cattggttcc cggttctgta ttgtttgtag atgatttggc tgttacaggc ggtgctgacg
     1441 ttgaagattg gaatggttat ggtattgctc ataatatcta tccaaaccct gccagcgatt
     1501 atatcaatat ccttaatcct gcaacaaacg atgtaactat cgaaatctat agtcttaccg
     1561 gtcagaaagt aggtgaacaa acacttgctc ctgacatgaa cagcatcaac cttgaaaact
     1621 atacaagcgg tatttatacc ttccgtttgg ttgacaatgg tactgttgtt tatagcagca
     1681 aatttgttgt gaagaaataa tcacataaac atattacaaa aaagtagccc cttcaaattg
     1741 aaggggcttt ctttttttaa tccaacacct atgaaattac ttgatctcga tttgcttttt
     1801 gttttctact tgcacggcgg ttttcaatgg caattcaata tgcagaatac catttgaata
     1861 agatgctgca atctgctctc catctatgtt ttcaggtaaa gtaaagctgc gttcaaaact
     1921 gccgaagctg aattcttttc gcgtgtaatt cttttctttt acctcatcct tggtctcttt
     1981 cttgcctgtg atgcgcaata catctttttc aacgtgcaca tcgaaatcac ccttgtcgaa
     2041 accgggtgcc gcaacttcca aatgataacc tttgtcgtgc tgctccacat tcactgaagg
     2101 cacgaaactg cctcggaaat ttgttgaagg cacattaaaa agatcgtcga acatgttgaa
     2161 catggtcgga attcctgaag gtctgcgttt tactgttaac atagtcatat gcgttttatt
     2221 ttgtttttga gttatacacg ctacccgaca aatgcagtac caagccaatt ttccaggcat
     2281 ttcacttaca attcgacaga atcaaattaa gcaatcggac aaaatggcgt attgctaact
     2341 agaaacaaga caaaatgacc tatttcgtta tgcgacctct gggtccaagt tcaatgacct
     2401 cgggttgcag tatttgcccg ttagaggcct gaaagcggat catggttctc agtttatgga
     2461 aaccgtgcac acctgatgcc cccggattca tgcataaggt gcccgtagtt tgatcgcgca
     2521 tcactttcag gatatgggag tgcccgcag
//
LOCUS       DAOQOU010000274         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_34008_length_2520_cov_5.496146, whole genome shotgun sequence.
ACCESSION   DAOQOU010000274 DAOQOU010000000
VERSION     DAOQOU010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34008_length_2520_cov_5.496146"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1783
                     /locus_tag="PLM90_14355"
     CDS             140..1783
                     /locus_tag="PLM90_14355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HMZ90615.1"
                     /translation="MSLSAISRLNDIRSAVEQHWDIIVIGGGITGAGIALEASCRGMK
                     VLLLEQYDFASGTSSKSTKLIHGGLRYLKQLEFGLVRTVGRERAVIHKLAPHLVIPTR
                     MVLPITEGGTLGKYSTSMALFVYDRLAGVKKHERYQMLTPQALHQMEPLLRSDNVKGG
                     AVYIEYRTDDARLTMAVLKSAHEKGAACFNYLKVTGFSQSNNHINGVEALDLLNNTKH
                     SFNTKLVINAAGPWVDDVRSLEELPHGKRLHLTKGIHIVIPASKFPLEHAIYFDVATD
                     DRMIFAIPRGEVVYVGTTDTNYRDSKEHILIDNADIDYLLSAITEMFPSIHLTRRDVE
                     SGWAGLRPLIHEEGKSPSELSRKDEIFVSKQGMISIAGGKLTGYRKMAEKAVRKAVDL
                     LRKRDGLQFPKANTAQLLLQGSHFSTSIDKYIALRAGEAKQAGISAEQVSRLVFTYGT
                     DAAEILDDVFAHIQRLQDPQQALLFAEVRYAIQNEMVTGVSDFLIRRTGSLYFNKRFA
                     TENYRLIAEIIADELQMDASDAKLQLQWFEAVLEELKFS"
     gene            1880..>2520
                     /locus_tag="PLM90_14360"
     CDS             1880..>2520
                     /locus_tag="PLM90_14360"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ90616.1"
                     /translation="MAAKRRNKPQEEKKIFILDTSVILHDHNSVNKFEEHDVVIPITV
                     LEELDNFKKGNETINFEAREFIRFLDNKSKESTLQEWRPINGKSTGRMIVMMYEDHGV
                     DAEVIFGEEKPDHKILNTALVVKHRYPEQTVVLVSKDINLRIKAKALGLLAEDYKNDK
                     IQDPEFLKEPIETIEGIDHGTIDKIYSDGFIGFKKVLKHEPYSNQCFILKNGS"
BASE COUNT          770 a          498 c          559 g          693 t
ORIGIN      
        1 ctaaccctca aacttgcaaa ctccgtccaa aatctccatt ccagtacaat cccattaact
       61 aatcagccaa ttccccctct ctcctatctc ctctctcctt tctcctcttt cctttctcct
      121 ttctccttaa ttttaccccg tgtccttatc tgcaatttcg cgtttaaatg atattcgcag
      181 tgctgttgaa cagcattggg atattattgt aataggagga ggcataacag gagctggaat
      241 tgcacttgag gcctcgtgta gaggcatgaa ggtgcttttg cttgaacagt atgattttgc
      301 aagtggcaca tcatcaaaat ctacaaagct gatccatggc ggattgcgct atttgaaaca
      361 acttgagttt ggattagtgc gaactgttgg acgtgagcga gcagtaattc ataagctggc
      421 acctcatcta gttattccaa cgcgaatggt tttaccgatc actgaaggcg gtacattagg
      481 aaagtattca acttccatgg cattgtttgt ttacgatcgc ctggcaggag taaaaaagca
      541 tgagcgctat caaatgctta caccacaggc cttacatcaa atggaaccgc tgttgcgttc
      601 cgacaatgta aaaggtggtg ccgtgtatat tgaatatcgc accgacgatg cccgattaac
      661 gatggcggtg ctaaaatctg ctcatgaaaa aggtgcagca tgttttaatt accttaaagt
      721 gacaggcttt tcgcaaagta ataatcatat taatggcgta gaagcgctgg accttttaaa
      781 taataccaag catagcttta acaccaaatt agtgataaat gctgccggcc cgtgggttga
      841 tgatgtgcgc tcattggaag agttgcctca tggtaagaga ttgcatctta caaaaggcat
      901 acatatcgtt atccctgcaa gtaaattccc attggaacat gcaatttatt tcgatgtggc
      961 aacagacgac agaatgattt ttgctattcc acgaggagaa gtggtctatg tcggcaccac
     1021 tgataccaat taccgcgaca gcaaggagca tattcttatc gacaatgctg atattgatta
     1081 tttgttaagt gcaattaccg aaatgtttcc gagtattcac ttaacgcgaa gagatgtaga
     1141 gtcgggttgg gcgggattgc gtccattgat acatgaagaa ggaaaatcac catcagagct
     1201 atcacgcaag gatgagatat ttgtttccaa gcaaggtatg atctccatcg ccggaggcaa
     1261 acttaccgga tatcgcaaga tggctgagaa ggccgttagg aaggcagttg atttgctcag
     1321 gaaaagagat ggattgcagt tcccgaaagc aaacactgca caacttttat tgcagggttc
     1381 gcatttctcg acaagcattg ataaatacat cgcacttcgc gccggagagg caaaacaggc
     1441 aggtatttca gcggaacaag taagcaggct ggtgtttacc tatggtaccg atgccgctga
     1501 gattctggat gatgtttttg cccatattca acgattgcaa gatccacagc aagctttatt
     1561 atttgcggag gtgcgatatg cgatacaaaa tgaaatggta accggcgtca gtgatttcct
     1621 gataagacgc accggcagtt tatattttaa caagcgcttt gcaacagaga attatcgact
     1681 aattgctgag atcatagccg atgaacttca aatggatgca tcagacgcaa agctgcaact
     1741 gcaatggttt gaggctgttt tggaagagct taaatttagc tgacctgtac gctgcatggt
     1801 acaattatca acaggatagt ggtataactg tctttggaat ttgctgtata gctactaaca
     1861 ttggcttaga aactaattta tggctgcaaa gaggcgtaat aaaccgcagg aagaaaaaaa
     1921 gatatttatt ctcgatactt cagtcatcct gcacgatcac aattcagtaa ataaattcga
     1981 agaacatgat gtagtgattc ctatcacggt gttggaggaa ctggataact tcaaaaaagg
     2041 taatgaaacc atcaattttg aagccagaga atttatccgc ttcctcgata ataaatcgaa
     2101 ggagtcgact ttacaggaat ggcgtccgat caacggcaaa tcgacgggca gaatgattgt
     2161 aatgatgtat gaagatcatg gcgttgatgc tgaagtgata tttggcgaag aaaaacctga
     2221 tcataaaatc ctcaatactg ccttggtagt caagcatcgt tatccggagc aaaccgttgt
     2281 attggtgagt aaggatatta atctgcgcat aaaagctaag gccttaggtt tgcttgccga
     2341 ggattacaaa aatgataaaa ttcaagatcc tgaattcctg aaagaaccaa ttgaaactat
     2401 cgaaggaatc gatcacggaa caatagataa aatctatagc gatggattta tcggattcaa
     2461 aaaggtttta aaacatgaac cttattccaa tcagtgcttt attctgaaga atggcagcag
//
LOCUS       DAOQOU010000275         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_34372_length_2506_cov_5.520604, whole genome shotgun sequence.
ACCESSION   DAOQOU010000275 DAOQOU010000000
VERSION     DAOQOU010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34372_length_2506_cov_5.520604"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1403
                     /locus_tag="PLM90_14365"
     CDS             270..1403
                     /locus_tag="PLM90_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ90617.1"
                     /translation="MKKLYISLIVGTLSMSVQAQIDPPCAIPGAFLCDNFDAYAAGDY
                     LGPNASWWTTWSGAEGTSEDAVVVSDYAYSGTNSMKIPGGGITDCILRLGDQSSGVWK
                     LEWMALIPDTHTGYYNIQESETPGVAWNLEVLYGLYDYTTPAPSGEGVVMVPVENNFS
                     YPVGEWFKVEHIIDLDANTFTLKINDVLVLDEAYTGNIGGIDFYSIDANNEQYIDDVL
                     LIELVPTTYYADTDGDTYGDPANTISVIGDAPDGYVSDNTDCDDTNAAVYPGATEVFN
                     GIDDDCDTQIDEGTVGITDITSGNIHVYPTPAQSYLNVEVPSNWNPSFTIQLFNNLGQ
                     LITTQMASGTSVTSIDVTNVAAGMYTIMATDENHVAAQAVIIE"
     gene            1619..>2506
                     /locus_tag="PLM90_14370"
     CDS             1619..>2506
                     /locus_tag="PLM90_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMZ90618.1"
                     /translation="MTKRNEIGIFGMPLNSMVLRLLFVLIWIILSYRIATAQTISSFA
                     PTTGPTGTVVTITGTGFDNTITNNVVFFGATQATVSSATITSLVVTVPASASYQYITA
                     TNLVTNLTAYSLQPFLYSDLCNSATNFASAVSVQGGSHNVEIYDMDGDGLPDLIIPDY
                     FNNYFTVTRNTSTPGTISFAPKVTFAWASGGQPNEVSIGDFDGDGKPDVALGGNGGGS
                     FVSVYRNTSTPGSISFAARQDFTASSGQGNGNRIIDIDGDGKPDLLSVHWSGNCLWVL
                     RNTSTGPGVISFAAGVSFST"
BASE COUNT          686 a          530 c          539 g          751 t
ORIGIN      
        1 taccgtcgat agagagggag ctgattgcgc ttcggaatta aattttatta aacaatataa
       61 aacgcattcc ctattaacgc gccagcgtta aaagcacccc acacttgcta acttgctaat
      121 tctcacattt tcccccactt gctgacttgc taactctcaa acttgctcac taaaactaac
      181 atctccatgc aagaacaccc ccatttgcta atcagctaat ttgctaattc tcacattatt
      241 ttgttaactt taagttaaaa gcaaaccgta tgaagaaact ctacatctca ctcattgttg
      301 gcacgctgag catgagcgtt caagcccaaa tcgacccgcc gtgtgcaatt cccggcgcct
      361 ttttatgcga taatttcgat gcctatgcgg ctggcgatta cctaggaccc aatgccagct
      421 ggtggacaac ctggagcggt gctgaaggca cttctgaaga tgctgttgta gtgtcggatt
      481 acgcttattc aggtaccaat agcatgaaaa ttcccggtgg tggtattact gactgtattc
      541 tccgtcttgg cgatcaaagt tccggtgttt ggaaactgga atggatggca ttgataccgg
      601 atacacatac cggttattac aatattcagg aatctgaaac gcccggcgta gcatggaacc
      661 tcgaagtgct ttacggactc tacgactata ctacccccgc accaagcggc gaaggagtag
      721 tgatggttcc cgttgaaaat aacttttctt atcctgtcgg cgaatggttc aaagtagaac
      781 acatcatcga cctcgatgcc aatacattta ctttgaaaat taatgatgta cttgttttgg
      841 atgaagccta taccggcaat atcggtggta tcgattttta ttccattgat gcgaataatg
      901 aacagtatat cgatgatgta ttactgatag aattagtgcc tactacttat tatgccgaca
      961 ctgatggcga tacctacggc gatcccgcaa atactatctc tgtaattgga gacgcacctg
     1021 atggttatgt tagtgataac accgattgcg atgatactaa tgcagccgtt tatcccggag
     1081 caacagaagt gtttaatggc atcgacgacg attgcgatac tcaaattgac gaaggaactg
     1141 ttggtattac tgatatcact tcaggcaata ttcatgtata tcctacacct gctcagtcct
     1201 atctgaatgt tgaagtgcct tcaaactgga atccaagctt taccattcaa ctgttcaata
     1261 atcttggcca gttaataacc actcaaatgg catcaggaac aagtgtaacc tcaattgatg
     1321 ttacaaatgt tgcagccggt atgtatacca taatggctac tgatgaaaac catgttgctg
     1381 ctcaggcggt aattattgaa taattatatt atcacacatt gcaatgccat cctccggggt
     1441 ggcatttttt ttgtggcgaa tcgcctgttt ttgttgttaa aaattactgc aaagtgagga
     1501 aagtcacttg attgccattg attttgttgt ctatttgctt atggataggc atttattgtc
     1561 agttccggaa tttagttcgg aaattgaaat atggtcacct tgaccctgtc cttggttgat
     1621 gacgaaacgc aatgaaatcg gaattttcgg gatgccgctt aacagcatgg tattgagatt
     1681 gctatttgta cttatctgga ttatattaag ctaccggatc gcaacagctc aaacaatttc
     1741 ttccttcgca cctactaccg gtcctacggg tactgtggtg acaatcacag ggacaggttt
     1801 cgacaatact atcactaaca atgttgtctt ttttggtgct acacaggcaa cggtgagcag
     1861 tgctacaata acgagtttag tggtcactgt tccggcgagt gcaagctatc agtatattac
     1921 ggctacaaat cttgttacca atctcacagc atattcactg caaccatttt tatattccga
     1981 tttgtgtaat agtgcaacca attttgcatc ggcagtgagt gtgcagggag ggtcgcacaa
     2041 tgttgagatt tacgatatgg atggagatgg attaccggac ctgattattc ctgattattt
     2101 taataattac tttaccgtaa ccagaaatac ctccacaccg ggaaccattt ccttcgcacc
     2161 taaagtaacg tttgcatggg cttccggcgg acaaccaaac gaagtatcga taggcgattt
     2221 tgatggcgat ggaaaaccgg atgtggcatt gggtggaaat ggcggcggtt cttttgtttc
     2281 tgtatatcgc aatactagta cgcccggcag tatttctttt gctgcgcgac aagattttac
     2341 ggcttcatca gggcagggaa atggaaatag aataattgat atcgatggtg atggaaaacc
     2401 tgatttatta tctgtgcatt ggtcgggcaa ttgtttgtgg gtattgagaa atacatctac
     2461 aggtcccggc gtaatttctt ttgcagcagg cgtaagtttc tccaca
//
LOCUS       DAOQOU010000276         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_36102_length_2439_cov_5.486577, whole genome shotgun sequence.
ACCESSION   DAOQOU010000276 DAOQOU010000000
VERSION     DAOQOU010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36102_length_2439_cov_5.486577"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..949)
                     /locus_tag="PLM90_14375"
     CDS             complement(239..949)
                     /locus_tag="PLM90_14375"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMZ90619.1"
                     /translation="MRSQLLFGIAICMLTITHLHAQTPQVDPAYIEVTGSAEMEIIPD
                     EIYISVSLSERYDGREKVTLAVQDEKFFNAIKELGIPMGDVQLSDAVTDYSYYKWRKE
                     DALATKDYIVLVHTAEMTSKLFKKLVDIDAENAKVTKVSHSKIEQYRKEVKIKAMKAT
                     HEKAEYMLEAIGQELGKPIVITELNENVLTTNYAQYDMNSYRTANFMLADGYTMPAEI
                     SFQKIKLKYEIYGKFEIK"
     gene            complement(1032..1385)
                     /locus_tag="PLM90_14380"
     CDS             complement(1032..1385)
                     /locus_tag="PLM90_14380"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HMZ90620.1"
                     /translation="MAYDTQLADRIYEILLAKEIPFTEKKMFGGIAYMVNDAMACGII
                     KKDLMIRCFHEDHEKLLKMQHVGEMKFTGKSMRGFLMIYPSGFETDAALGYWLQHGVN
                     YALLAPKKPKKSKKK"
     gene            complement(1388..2341)
                     /locus_tag="PLM90_14385"
     CDS             complement(1388..2341)
                     /locus_tag="PLM90_14385"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HMZ90621.1"
                     /translation="MNKALTKLTMLAVALSMFSCEKTVYFEVETQENLLVVNALIIPD
                     SVNDATISLSVDPIAIGFDAASVSDATVDVYRNDVLSGTFISEGGGYYTLPPTVLAGA
                     DGEEVRFEVSAPGRESVSATTTIPTLVPITSLEITDTLYETISYSVIDDEGNVTYIDT
                     IVPYFNLELTFDDPIGEDHYMLDIDYQDAFSYVQACYTTNDPVFTVESSYGYGGANDD
                     GSVTFCGDVVFSDQSFEGKTKTISALVYAIETTFTIDPTFVVTLKHISDDYYKYYTTS
                     QQQYYNNGDPFSEPTVVFDNITGGFGIVAGYTVSKSTLPLD"
BASE COUNT          709 a          544 c          410 g          776 t
ORIGIN      
        1 tcgtctataa tatcaaaggc ggctttgttg aagtagtgaa taataccgtt agcgtactag
       61 tagaaggcgt agaaaaagcc taataaaaaa tatacaatct caaagccccc tgatatcaat
      121 cagggggctt tttttattac tatcaatacc attcaaccgc aaaataaact cacccacccg
      181 cctactcggc gaagtaaact caccaacttt ctcctttctc ctttctcctc tctccttttc
      241 atttaatctc aaacttccca taaatctcat acttgagttt tattttttgg aaagagatct
      301 ctgcaggcat ggtgtaacca tcggctaaca tgaaattggc agtcctgtag gaattcatgt
      361 catactgcgc ataattggtg gttaacacat tttcattcag ctccgtaatt acaatcggtt
      421 taccaagttc ctgtccaatg gcctcgagca tatattccgc tttttcatga gtagccttca
      481 tcgcttttat ttttacttcc ttgcgatatt gctcaatctt tgaatgactt accttggtaa
      541 ccttcgcatt ttcagcatct atgtcaacca attttttgaa tagcttggat gtcatttctg
      601 cagtgtgcac caatacaata taatctttgg tggcaagcgc atcttcttta cgccatttat
      661 aatagctata atctgtaaca gcatctgata actgcacatc acccattggg atacctaatt
      721 cctttatcgc attaaagaat ttttcatcct gcacggcaag tgttactttt tcgcgaccgt
      781 catacctttc agatagagaa actgatatat aaatttcatc gggaattatt tccatttcag
      841 cactgccggt tacttcgatg taagcaggat caacttgagg agtttgtgca tgtaaatgtg
      901 taattgtaag catgcaaatt gcaatgccga agagtagttg agatcgcatg attcctgttt
      961 ttattgaatg gcaataccgc catcgaaacg aacgcatgaa acttctgttt attgcaaaat
     1021 acagggtaat cttacttttt cttagacttt ttgggcttct taggagcaag caaagcataa
     1081 tttacaccgt gttgcagcca atatcccaaa gcggcatctg tctcaaatcc tgaaggatat
     1141 atcatcagaa atccacgcat cgacttgccg gtgaatttca tttcacctac atgttgcatc
     1201 ttcagtaatt tctcatgatc ctcatgaaaa catctgatca tcagatcttt tttgataatg
     1261 ccgcaagcca tagcatcatt taccatataa gcgattcccc caaacatctt tttttctgta
     1321 aagggaattt cctttgccag caaaatttca tatatgcggt ctgcaagttg cgtatcgtat
     1381 gccataatta atcgagggga agtgtcgatt tactaacggt ataacccgca acaataccaa
     1441 aaccgccggt aatattatca aatacaacag taggttcgct gaacggatcg ccgttgttat
     1501 aatattgttg ttgactagtt gtgtaatatt tataataatc gtcagaaata tgtttcaacg
     1561 ttaccacaaa tgtagggtca atagtaaagg tagtttcaat tgcatatact aaggcgctaa
     1621 tggtttttgt ttttccttca aacgattgat ctgaaaaaac tacatcaccg caaaaagtta
     1681 cactgccatc atcatttgct ccgccatagc cataacttga ctcaacagta aatacagggt
     1741 cgttggtagt gtagcatgcc tgaacatagc taaatgcatc ctgataatca atatcaagca
     1801 tataatgatc ttcaccgata ggatcgtcaa aggtgagttc aagattgaaa taaggaacaa
     1861 ttgtatcgat ataagttaca ttgccttcgt catctattac actatacgaa atcgtttcat
     1921 acaaggtgtc tgtgatttca agcgatgtaa ttggcactaa tgtaggaata gttgtggtag
     1981 ctgataccga ttcgcgaccg ggagcactta cctcaaaacg cacttcttcg ccgtcggcac
     2041 ccgccaatac tgtcggagga agtgtataat aacctccgcc ctctgaaata aatgtacctg
     2101 acaacacatc attgcgatac acatcaacgg tagcatctga aactgaggca gcatcaaaac
     2161 caattgcaat aggatctaca ctcaaagaaa tagttgcgtc atttacactg tcgggaatga
     2221 tcaatgcatt cacaaccaac aggttttcct gtgtttctac ctcaaaatac acagtcttct
     2281 cgcagctaaa catgctcaat gcaactgcta gcatcgttaa ttttgtcagc gctttattca
     2341 ttgtatttat cttgttatcg tgtcctgtaa aaaaaacctc aagccctctc tcctttctcc
     2401 tttttcctct ctcctttctc ctttttcctc tctcctttc
//
LOCUS       DAOQOU010000277         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_36103_length_2439_cov_5.468540, whole genome shotgun sequence.
ACCESSION   DAOQOU010000277 DAOQOU010000000
VERSION     DAOQOU010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36103_length_2439_cov_5.468540"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLM90_14390"
     CDS             complement(<1..1340)
                     /locus_tag="PLM90_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMZ90622.1"
                     /translation="MAFKTIIEPFRIKTVEPIKMSSVAERKKYLKAAHFNPFLLHSSQ
                     VIIDMLTDSGTSAMSQNQWAAIMQADESYAGAQSWERFYAAVSDLTGCEFVLPTHQGR
                     AGERILYGYLGGKGKVFISNTHFDTTRANIEFSGAEAFDIPCKEADDFDSNHPFKGNM
                     DVEKLEKLLKQHKKNVAAVIMTVTNNSGGGQPVSMANMESVSALCKKYKVLLILDCCR
                     VAENAWFIRHKEPGFENMSYRDIAKKMFDMADGSVMSAKKDALVNMGGWLTLRDKKIA
                     DACMGLLIITEGFSTYGGLSGRDMEAVAVGIKEVFDDDYLNYRIKSTAYLGEHLRNNN
                     IPIMYPTGGHAVYVDAAKLYPHIPVHQYPGQALACALYTIGGVRSVEIGSVMFGKYDN
                     KKKLIPARHELVRLAIPRRVYTQSHIDYVIEVFREVEKNKLRAKGLKIIYEQAFL"
     gene            complement(1536..>2439)
                     /locus_tag="PLM90_14395"
     CDS             complement(1536..>2439)
                     /locus_tag="PLM90_14395"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90623.1"
                     /translation="IDNPDYAPVVNYLQQFLPDSSLYLETTYKAVITNNSLACLLEHN
                     DTLFLAFDWYDENGIAIAPDPGYVYKWTNNLTDDILIGTAVNFMCNWISDDDFNAGNR
                     LMIYLQVYDTSGALIAFDTKYFYVNPDFAPTCSFEMFGDGELTVSFTDLEITGNYTCY
                     WWEINGDSMFYNSMPGPFTFEASGAYAITLHVQYGNTPCIKSFTRTFISDEIQTNNID
                     NFLVYPNPANTILRIEFPNGAYDGHLELSDINGKIVRSVELNADSILYYDIDVANLAS
                     GLYVLHFYGNATEFGTPVVIMHNK"
BASE COUNT          729 a          523 c          467 g          720 t
ORIGIN      
        1 agaaaagcct gctcatagat gattttcagg ccttttgctc tgagtttatt tttttctact
       61 tcgcgaaata cttcgatcac ataatcaata tgactttggg tgtacacgcg tcgcggaatg
      121 gcgagacgaa cgagttcgtg acgtgcgggg ataagtttct ttttattatc gtatttgccg
      181 aacatcacag agccaatttc cacgctgcgc accccaccga ttgtgtacaa agcacatgca
      241 agcgcttgtc cggggtattg atgtacagga atatggggat ataatttagc cgcatcaaca
      301 tacaccgcat gcccccctgt cggatacatg ataggaatat tattattgcg cagatgttcg
      361 ccgagatagg cggtgctttt aattctataa ttcagataat catcatcaaa aacttccttg
      421 ataccaacag caaccgcttc catatcgcgg ccactgagtc cgccataagt tgaaaaacct
      481 tcggtaataa taaggaggcc catacaggcg tcggcaattt ttttatcgcg tagtgtcagc
      541 caaccaccca tatttacgag tgcatctttt ttagcactca tcacagaacc atcggccata
      601 tcgaacattt ttttggcaat atcgcgatac gacatatttt caaaaccggg ctctttatgt
      661 ctgatgaacc acgcattttc ggcaacgcga caacaatcaa gtatcagcaa taccttatat
      721 tttttgcaga gcgccgacac gctttccatg tttgccattg aaaccggctg accaccaccg
      781 ctattattgg ttacggtcat aattactgct gcaacatttt tcttgtgttg cttcaacaat
      841 ttctctaatt tctcaacatc catattccct ttgaaagggt ggttgctgtc gaaatcatca
      901 gcctctttgc aaggtatatc gaaagcctcg gcgccgctga attcgatatt tgctcttgtg
      961 gtatcgaaat gtgtattgct gataaatact tttcctttcc cacccagata accgtataaa
     1021 atacgttccc ctgccctacc ctgatgcgtt ggtaatacaa actcgcaacc ggtaaggtcg
     1081 cttacggcag catagaatcg ttcccagctt tgcgctcctg cataagattc atcagcctgc
     1141 ataatggccg cccactgatt ctgactcata gccgaagtgc ccgagtccgt aagcatatca
     1201 atgatcacct ggctggaatg gagcaggaaa ggattgaaat gagcggcttt caggtatttt
     1261 ttcctttctg caacagagct cattttaata ggctctacag tctttattct aaaaggctcg
     1321 atgatagttt tgaatgccat aatacaaatt tgtagcgcaa agatgaggcg aatgcagttg
     1381 aaccaaggtg acttatgtca atgcaaagta atttgaatgc gaagcagagc ctatcatgta
     1441 atcatgcaaa acgattgcta tctacacttg atttcacaat gtttcgaaga attttaattg
     1501 atttgagtta cgcagagtat tcgaaaaagc aaatcctatt tattgtgcat aatcacgaca
     1561 ggagtaccaa attctgttgc atttccataa aaatgaagga cgtataatcc tgaagccagg
     1621 tttgcaacat cgatatcata atatagaatg gaatcagcat tcaattcaac agaccgaaca
     1681 atttttccgt ttatatcaga taattctaaa tgtccgtcat atgcaccatt tggaaattct
     1741 atgcgaagaa ttgtatttgc cggattagga tacaccagaa aattatcaat gttatttgtc
     1801 tgaatttcgt cgctaataaa agtccgtgta aaggatttga tacatggtgt gttgccatat
     1861 tgcacatgca aggtaattgc ataagcaccg gaggcttcaa aagtaaaagg gcctggcatc
     1921 gaattgtaaa acatgctatc accattaatt tcccaccagt agcaagtata atttccggta
     1981 atttccaaat ctgtgaacga gacagtcaat tcaccatcac caaacatttc aaaagaacag
     2041 gttggagcaa aatccggatt tacataaaag tattttgtat caaatgcgat caaggctcct
     2101 gaagtgtcat aaacctgcag atatatcatg agtctgtttc cggcattaaa atcatcatct
     2161 gaaatccagt tgcacataaa attcactgca gtgcctatga gtatatcatc agttaaatta
     2221 ttcgtccatt tatatacata tccggggtca ggtgcgatag caatgccatt ttcatcatac
     2281 caatcaaacg ccaagaaaag tgtatcatta tgctcgagta agcaagcgag gctattattt
     2341 gtaataacag ctttatatgt tgtctccaga taaagactac tatcaggcaa aaattgttgc
     2401 aagtagttta ctacaggggc ataatctgga ttatcgatt
//
LOCUS       DAOQOU010000278         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_36227_length_2435_cov_3.563025, whole genome shotgun sequence.
ACCESSION   DAOQOU010000278 DAOQOU010000000
VERSION     DAOQOU010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36227_length_2435_cov_3.563025"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLM90_14400"
     CDS             <1..309
                     /locus_tag="PLM90_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90624.1"
                     /translation="GIQYHAFSQQGEAFNTSTGVKSKWLQQLLLNREHPLRKTLTFFI
                     RPFRNLIIKSGIIDKIYKLNKKQVELPKLTLEERQIVAPYFEQDLKQLESDWHVTFQR
                     "
     gene            339..1394
                     /locus_tag="PLM90_14405"
     CDS             339..1394
                     /locus_tag="PLM90_14405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HMZ90625.1"
                     /translation="MSKRFLFLISAVALLAACSKSKESSQSAAPAGPPPAQPVKAIIA
                     TTSSLDNHIEATGTILANEEVEIRSETSGRVIKLYFKEGDVVNAGSTLLTIDDRELQA
                     QLTKTNLSIDLATDDEQRKKKLLDVGGISKEEYDASRNYLLKLEADKQLLETQIAKTK
                     VTAPFSGTIGLRYISEGGYVSPATLIASLQQTNMVKVEFSVPEKYASSLKNNATVNFT
                     VEGDDHTFEAKVYAREPKIDPQTRSIKVRAIALNAEQRLIPGAFAKLTINLGTIPEAI
                     EVPSEAIIPAITGQTLYVVRAGTAKVQPIKTGIRTATTTQITEGIVPGDTIITTGLLT
                     VRPGAAVKPIIVENKKD"
     gene            1399..>2435
                     /locus_tag="PLM90_14410"
     CDS             1399..>2435
                     /locus_tag="PLM90_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ90626.1"
                     /translation="MNISSVSINRPVLATVISILILLFGIIGYSFLGVREFPSVDPPI
                     ITVTTNYTGANADIIESQITEVLEEQINGIAGISSLSSTSSDGRSTITVEFTLDVDLE
                     AAANDVRDRVSQAARNLPPDCDPPIVAKSSADSNPIVSMTIQSDQRSLLELSEIANNV
                     FKERLQTIPGVSEVRVWGEKKYSMKLLLDPVKLAGYHLTPSDVRDALNRENVELPTGR
                     IEGYSTELSIRTFGRMTTEEEFNDLIIREQDGQVIKLQDVGQAKLMPENERTVLRGNG
                     GIPQVAVAITPQPGSNYIEIADEYYRRFDQIKKELPDDLQYKIALDTTTGIRKAIDEV
                     KETLLIAFGLVV"
BASE COUNT          769 a          549 c          498 g          619 t
ORIGIN      
        1 ggcattcaat atcatgcatt cagtcaacaa ggtgaggcct ttaatacctc caccggtgtc
       61 aagagtaaat ggctgcaaca actgttactc aatcgcgaac atcctttgcg caaaacatta
      121 acctttttta tccgcccttt caggaaccta attatcaaaa gtggtattat tgacaagata
      181 tacaaactca ataagaaaca agtagaactc cctaaactca ccctagaaga aagacaaatc
      241 gtagcaccat atttcgaaca agatttaaaa caactcgaat ccgactggca tgtcacattt
      301 cagcgatagc aatgccggcg ttttcacgaa aaaatagtat gagcaaaaga tttttatttt
      361 taattagtgc ggtagccctt cttgccgctt gttccaaatc aaaagaatcg tcgcaatccg
      421 cagcacctgc cggaccgcct cctgcacaac ccgtcaaggc tattatcgca acaacctctt
      481 cgctcgataa ccacatcgag gcaaccggga ccattcttgc aaatgaggaa gtggaaattc
      541 gcagcgaaac ttccggtcga gttataaaat tgtattttaa agaaggcgat gttgtcaatg
      601 caggaagcac tctgctcaca attgatgaca gagaattgca ggcacaactc actaaaacga
      661 acctttccat cgacctggct acagatgatg aacaacgtaa gaaaaaatta ttagacgttg
      721 gtggaatttc taaagaagaa tacgatgcct caagaaatta tctgttaaaa ctggaagctg
      781 ataagcaatt attggaaacg caaattgcga aaacaaaagt aactgctcca tttagtggaa
      841 ccatcggact gcgttatatt tcagaaggcg gttatgtttc tccggccaca ttgatcgcca
      901 gcctgcagca aacaaatatg gtgaaagttg aattttctgt tcccgagaaa tatgcatcat
      961 ccttaaaaaa caatgcaacg gtaaatttca cggttgaggg cgacgaccac acatttgaag
     1021 ccaaggtcta tgctcgcgaa ccaaaaatcg atccgcaaac caggtctata aaagtacgtg
     1081 ctattgcttt gaatgccgag cagcggttga ttcccggtgc ttttgcaaaa ctcaccatta
     1141 atctcggcac tattcccgaa gcaattgaag taccttcaga ggctatcatc ccagccatca
     1201 caggacaaac attgtatgta gtgcgcgccg gaacggcaaa ggtgcaacct atcaaaaccg
     1261 gtattcgcac agctactact actcagatca ctgaaggtat tgttcccggc gataccatta
     1321 ttaccaccgg attattaacg gttcgacccg gtgccgcagt gaaaccaatt attgtggaaa
     1381 ataaaaaaga ttgatggcat gaatatttct tctgtatcca ttaatcgccc cgttttagca
     1441 acggtaatca gtattttaat tctgttattc ggaattatcg gttactcatt tctgggcgtc
     1501 cgcgaatttc ccagcgtaga tcctcccatc attaccgtaa ccactaatta taccggtgcc
     1561 aatgccgata tcatcgaatc gcaaatcacc gaagtgctcg aagaacaaat aaatggtatc
     1621 gccggtatca gttctttatc atctacatca tcagatggtc gaagtacgat tactgttgaa
     1681 tttactctcg atgttgatct tgaagctgca gcaaacgatg tgcgcgaccg cgtgagtcag
     1741 gcagctcgaa atctgccacc ggattgtgat ccgcctattg ttgctaaatc tagtgccgat
     1801 tcaaatccga tagtgtccat gaccattcaa agcgatcaac gatcattatt ggaattatcg
     1861 gaaattgcca ataatgtatt taaggagcga ttgcaaacta ttccgggtgt aagtgaagtg
     1921 cgcgtatggg gtgagaaaaa atacagcatg aaattattgc tcgatcctgt taaactcgcc
     1981 ggatatcatc tcacgccatc agacgtacgc gatgcattga acagagaaaa tgttgaactg
     2041 cctacaggtc gcattgaagg atattccact gaattatcta tccgcacttt cggtcgcatg
     2101 accactgaag aggaattcaa cgaccttatc atacgcgaac aagacggaca ggtaattaaa
     2161 ctgcaagatg ttggtcaggc gaaattgatg cctgagaacg aaagaaccgt attgcgtggc
     2221 aatggtggca tcccacaggt agcagtggct atcacaccgc aaccggggtc aaattatatc
     2281 gaaattgctg atgaatatta tcgccgcttc gatcagatta aaaaagaatt accggatgac
     2341 ctgcagtata aaattgcatt ggataccacc accggcatcc gcaaagcaat tgatgaagta
     2401 aaagaaacat tattaattgc attcggactg gtagt
//
LOCUS       DAOQOU010000279         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_36391_length_2429_cov_6.001685, whole genome shotgun sequence.
ACCESSION   DAOQOU010000279 DAOQOU010000000
VERSION     DAOQOU010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36391_length_2429_cov_6.001685"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1664)
                     /locus_tag="PLM90_14415"
     CDS             complement(114..1664)
                     /locus_tag="PLM90_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HMZ90627.1"
                     /translation="MKNGITILWADDEIDMLKPQIMFLEEKGYDVVAVSNGLDAVDKC
                     VSPELDLIFLDEGMPGLSGLETLQLIKEKRPSIPIVMITKNETEGLMEEAIGSQISDY
                     LIKPVKSQQILLTLKKLIDNKRLVTEKTDSNYQKEFQKLFFSLQDANDSEQWFDIYKK
                     LVHWEIQLDKTGTAGMKEVLTSQKAEANVEFGKFIDKNYLSWMAGKGKRPVMSHTLMQ
                     EKVFPLLQDKVPTFFILIDNLRFDQWKVIEPLISESFKLKSEDYFFSILPTATQYARN
                     AIFAGLTPAEIEKRLPSLWLNDEEEGGKNMQESEFLKDNIVRNKMNVKYSYHKILNYS
                     EGKNLEDNILNLLNNDFNAIVYNFVDMLSHARTEMEVLKELASDETAYRSLTRSWFDH
                     SPLHDALRKLSSKKVRIIITTDHGTIRVHTPSKVVADRNTTTNLRYKSGKNLQYNKKD
                     VFEVKVPAEAGLPKQHVTSSFIFAKEDAFFAYPNNYNYYVNYFKNTFQHGGISLEEMI
                     IPFAVFES"
     gene            1766..>2429
                     /locus_tag="PLM90_14420"
     CDS             1766..>2429
                     /locus_tag="PLM90_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ90628.1"
                     /translation="MNKRKIINDPVHGFITIPHEIIFDLIEHPWFQRLRRIQQLGLSH
                     LVYPGAIHTRFNHALGAMHLMGKAIHVLRQKGQQITEEESEAAAIAILLHDIGHGPFS
                     HALEKTILSNVHHEEVSIRFMERLNEQFQGRLSMALEIFRDTYPKRFLHQLVSSQLDV
                     DRLDYLTRDSYFTGVHEGVIGYDRLIEMMDVENDELVIEHKGIYSVEKFIIARRVMYW
                     QVY"
BASE COUNT          663 a          543 c          470 g          753 t
ORIGIN      
        1 tgatacttga tacctgatac ctgatacttg atacctgata cctgatactt gatacctgat
       61 acctgatact tgatacctga tacctgatac ctgaatcttc catccaacaa ccctcaactc
      121 tcaaacaccg caaacggaat aatcatctcc tccaaactaa tacctccatg ttggaacgta
      181 tttttgaaat agtttacata gtaattgtag ttattcggat aagcaaaaaa agcatcttcc
      241 tttgcaaata taaaggagct ggttacgtgt tgctttggta aaccggcttc tgcaggcacc
      301 tttacttcga acacgtcttt tttattgtat tgcaaattct ttccactctt gtaacgcaaa
      361 ttcgtggtgg tgtttctatc tgctacaacc ttgctcggag tatgcactct gatagtgcca
      421 tgatcagtag tgatgataat gcgcactttc ttcgagctca atttgcgcaa agcatcgtgc
      481 aatggcgagt ggtcgaacca tgaacgggtg agcgagcgat atgctgtttc atcgctggcg
      541 agttctttta atacttccat ttctgtgcgc gcatgcgaca gcatgtcgac aaaattgtaa
      601 acaatagcat taaaatcgtt attcaataaa ttcaaaatat tatcttcaag attctttcct
      661 tcggaatagt tgagaatttt gtgataactg tattttacat tcattttatt gcgcacaata
      721 ttgtctttca ggaattcaga ttcctgcata ttctttcctc cctcttcctc atcattcaac
      781 cacaatgaag gcaatcgttt ttcaatttca gcaggcgtca gtcccgcgaa aatggcattg
      841 cgtgcatact gtgtggctgt tggtaaaatg ctaaagaaat aatcttccga ttttaatttg
      901 aaagattcac tgatcaatgg ttctatcacc ttccattggt cgaagcgcaa attgtcgata
      961 agaataaaaa atgtaggcac cttatcctgt aataatggga acactttttc ctgcatcagc
     1021 gtgtgcgaca ttaccggacg cttgcctttt cccgccatcc acgacaaata attcttatcg
     1081 atgaatttcc cgaattcaac attggcttct gctttctgag aagtcagcac ttctttcatt
     1141 cccgcagtgc cggttttatc gagttgaatt tcccagtgaa cgagtttttt ataaatatca
     1201 aaccactgct ccgaatcgtt ggcatcttgc agtgaaaaaa atagtttttg aaattccttc
     1261 tgataatttg aatcggtttt ctcggttacc aatcgcttgt tgtctatcaa ctttttgagc
     1321 gtaagtaata tctgttgcga tttcacaggt ttaataaggt aatcactgat ctgcgagcca
     1381 atggcctctt ccatcaaacc ctccgtttca ttcttggtaa tcatcacaat cggtatggaa
     1441 gggcgctttt ctttgatcag ttgtaaagtc tccaatcccg acaagcccgg cattccttca
     1501 tcaagaaata tcagatccaa ttccggactg acacatttgt ctacagcatc cagtccattg
     1561 ctcacggcta caacatcata tcccttctct tcgaggaaca tgatctgtgg ttttagcata
     1621 tctatctcat catcagccca taaaatggtg attccgttct tcataaatcc ctaattttgc
     1681 gttgttgtta ttggtagcgt aaagatactt cgataaatac ttaggcgttg gcgcagcctc
     1741 tcacatcacg ataaaaactg cagtattgaa taaaagaaag atcattaacg atccggttca
     1801 tggatttatt accatccctc atgagatcat tttcgacctc atagaacatc catggttcca
     1861 gcgtttgcgc agaattcagc agctgggact cagtcatttg gtataccccg gtgctatcca
     1921 cacacgcttc aaccacgcat tgggcgccat gcacctgatg ggtaaggcaa ttcatgtgtt
     1981 gcgtcagaaa ggtcagcaaa ttacagaaga agagtcggaa gccgccgcaa ttgccatctt
     2041 gttgcacgat atcggtcacg gtcccttttc gcatgctctt gagaaaacta tattgagtaa
     2101 tgtgcatcac gaagaggtgt ccattcgctt catggaacgc ctgaatgagc aatttcaggg
     2161 gcgattgtca atggcactcg agatattcag agatacttac cctaagcgct ttttacacca
     2221 gctggtgagc agtcagctcg atgtcgacag gctcgattat ctcacccgcg acagctattt
     2281 taccggtgtg catgaaggcg taatcggtta cgacagactc atagaaatga tggatgttga
     2341 aaatgatgaa ctggtgatcg agcataaggg catttactct gtagaaaagt tcattattgc
     2401 cagaagagtg atgtattggc aggtatatc
//
LOCUS       DAOQOU010000280         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_36938_length_2409_cov_4.745964, whole genome shotgun sequence.
ACCESSION   DAOQOU010000280 DAOQOU010000000
VERSION     DAOQOU010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36938_length_2409_cov_4.745964"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="PLM90_14425"
     CDS             <1..2278
                     /locus_tag="PLM90_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ90629.1"
                     /translation="TPLSDPKSNFREWLTTKAGEAPVLIDSTIADETAVRMSNYLYNR
                     GFFEGTAVAGYRYDKKRQTGKVIYEVDAGKLYRMRNVYYEIADKGLLNKINQIPQKSS
                     LAPGEPIDVDLLKAERTRINDYLRNVGYYRFQKEYIYFEVDTASGFDSLDIYVRVSNP
                     VSDTVHHPFRIRNIYVYPNASIDYVDGVTPTNTFHYTDSTKVSRPSKKKLIAQYASRD
                     TTLASASMYSDYLKGKVNKKDALKYYINKDSSSRKYYTLTAAGDLQPVKLYKTRTDYY
                     LINSLDNYSPKAIADNIFINPNGYYSDSLIQRTVASFSSVGVFKYITVQALEIWDSTS
                     YQQSIDLIVRLDPLQIRTVSYEFNANTTSDYLLGNAINFGYNQKNLFHRMDQLKLSLK
                     GGIETQLGGDVTYINTSELNAGLSLTLPEFMWPIPIQVPKRYFPKTDWRVNFNYIDQI
                     NDFTLFNTTIEYNVSIYENSKNDRAQKQHIFKMPIPTLNIVRVPNISDAFREELNANP
                     LLRQSFEEQLILGYGYTLILNTQPTGKHVLDYYLRTSAEFNTPFSDFIKVDADFRTYL
                     NINTSNNIVFRATSGVAIPLAGKNPDAMFNTEVIPYVKQFFTGGAYSVRAFTVRKLGP
                     GAYVDYDTTNFQRVDQVADIKLEANLEYRFDIISMLEGAVFCDIGNTFTLKDDPFRPH
                     AQFHFTDFYKLLAVGPGAGLRLDFGYFVIRVDAAYPLYDPALDGPYREEVLGAYEAAG
                     YTVPSKKVAINLAIGYPF"
BASE COUNT          695 a          512 c          508 g          694 t
ORIGIN      
        1 cactcccctc tccgacccta agagtaattt tcgcgaatgg cttacaacca aggccggaga
       61 agctcccgtg ttaatagata gcacgattgc tgatgaaaca gcggtgcgca tgagtaatta
      121 tttatacaat cgcggatttt ttgagggcac tgctgttgca ggatatcggt atgacaagaa
      181 aaggcaaacc ggtaaagtga tatatgaagt tgatgccggc aagttatatc gcatgcggaa
      241 tgtttattat gaaattgccg ataaaggctt gttgaataaa ataaatcaaa ttcctcaaaa
      301 aagcagtctg gcacccggtg aaccgataga tgttgatttg ttgaaagccg agcgcacccg
      361 cattaacgac tatctgcgta atgtaggtta ttaccggttt caaaaggaat acatctattt
      421 tgaggtagat actgcttccg ggtttgacag tctggatatt tatgtgcgcg tttcaaatcc
      481 ggtgagtgat accgttcacc acccttttcg catcaggaat atttatgttt atccgaatgc
      541 ctctatcgac tatgtggatg gtgtgacacc aactaatact tttcactata ccgatagcac
      601 aaaagtttcc agaccatcta aaaagaaatt gattgcacaa tacgcaagca gagacaccac
      661 acttgcttct gcaagtatgt atagtgacta tttaaaaggc aaggtaaaca aaaaggatgc
      721 cttgaagtac tatatcaata aagattcatc atcgaggaaa tattataccc taacggcagc
      781 cggtgattta cagcctgtaa aattgtataa gaccagaacg gattattatt tgatcaattc
      841 gctggataat tatagtccga aggccattgc ggataatatt ttcataaacc ccaacggcta
      901 ttattccgat tcattaattc agcgcacagt tgcttctttt tcgtcggttg gcgtatttaa
      961 atacattacg gtgcaggcct tggaaatttg ggattctacc agttatcagc aaagcataga
     1021 tttgattgtg cgattagatc cattgcaaat tcgcacggta tcgtatgaat ttaatgccaa
     1081 caccacttca gattacttat taggaaacgc cattaatttc ggttacaacc agaaaaatct
     1141 atttcataga atggatcaac tgaaactgag tttaaaagga ggtattgaaa cgcaattagg
     1201 tggtgatgtc acctatatca acacctcgga actcaatgcc ggcttgagtc tcacgctacc
     1261 tgaatttatg tggccgatac cgatacaagt gccgaaacgc tattttccaa agacagactg
     1321 gcgagtaaat ttcaattata ttgatcagat caatgatttc actttattta ataccacgat
     1381 cgaatataat gtttccattt acgagaattc caaaaacgat cgtgctcaga agcagcatat
     1441 tttcaaaatg ccgattccta cactgaatat cgtgcgcgta ccaaatatca gcgatgcctt
     1501 tcgtgaggaa ttgaatgcaa atccattatt gcgtcaatca tttgaagagc agctcatcct
     1561 tggatacgga tatacactga ttttaaatac ccagccgaca ggcaagcatg tgctcgatta
     1621 ctacctccgc acgagtgccg aattcaatac accattcagt gattttatca aagtagacgc
     1681 ggattttcgt acctatctga atatcaatac ttccaacaat atcgtgttca gagcaacctc
     1741 aggtgtagct attccgcttg caggtaaaaa tcccgatgcc atgttcaaca cagaagtaat
     1801 tccttacgta aaacagtttt tcaccggagg ggcttacagt gtcagagcgt ttactgtgcg
     1861 caagcttggt cccggtgcct atgttgatta cgatacgaca aatttccagc gcgtagatca
     1921 ggttgctgat attaagctcg aggccaatct cgaataccgt ttcgatatca tctccatgct
     1981 ggaaggggcg gtattttgtg atatcggtaa cacctttacg ttaaaagatg acccattccg
     2041 accacatgca cagtttcatt tcactgattt ctacaaactg ctggctgttg gaccaggagc
     2101 cggattgcga ctggatttcg gttacttcgt gatacgtgtt gatgccgctt atccattgta
     2161 tgatccggca ttggatggtc cgtatcgaga agaggtatta ggcgcatatg aagctgccgg
     2221 ttataccgtt ccttcgaaaa aagttgcaat caatctggct attggatatc cgttttaacg
     2281 gatcgggtgt tgcgcacttc cggagcaggt ttgcaaatag taatcatttc gcgctgcctg
     2341 cccacttgct ctggatcagg aacagctgct ttgcgctcgg gccctttgtt ttgcgagccc
     2401 tctgcgcct
//
LOCUS       DAOQOU010000281         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_37783_length_2380_cov_5.267527, whole genome shotgun sequence.
ACCESSION   DAOQOU010000281 DAOQOU010000000
VERSION     DAOQOU010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37783_length_2380_cov_5.267527"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="PLM90_14430"
     CDS             <1..1783
                     /locus_tag="PLM90_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90630.1"
                     /translation="KTGFCLLYFCKVKKGFRHITSVIAFVVIAFASTILQAQTYNIAT
                     YNGMTVTTCSGNFYDSGGSGGNYGNNENYTVTFCSASGSPLYFDFSSSSSALNLDAGT
                     GDTLFFYDGLTTSDPLIATLVKADDYAFSYPAVGTFSSCVTIKWKSNASAVDGGWQAS
                     ISCEEPPTCADNPPAADVFGQATAVCNLNDYCGTTSRYYGEDAPFNFGGTGGSCPGPD
                     DGLFAGTLENNSWLKFIADATSASFDFNVTGGASCDGVQVGIFSFDAATNTFARKSPC
                     NYTDGGWSGAFTLTATGLTVGQTYYMMIDGNAGSNCDYTIGAGTGVATVSAGADQIVC
                     AFNTTLAATTAMGSGTWSVVSGTGVFADATNPTTYVSGLSYGINVFRWTSTGGLCMDV
                     GAYDDVVVENTCLLPITLSDFTATVVDDMVRLNWTTASEIDNDHFVLERAGADQQFMA
                     IAEIMGSGTTTIPVNYTYTDDMPLPAVNYYRLKQVDVNANTSTSNVIAIESHSDAQTF
                     TIGDCYFDNASEVLTIPYQTSASVALDIQLCDIAGRILFSATCHTTAVNDNAQVDLHH
                     LTQTGLILLQITGDNGVRSNHTFWISE"
     gene            complement(1920..>2380)
                     /locus_tag="PLM90_14435"
     CDS             complement(1920..>2380)
                     /locus_tag="PLM90_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMZ90631.1"
                     /translation="LTFTWLYQDADGDLFGNANIDTLTCMEISGYVLDSTDCNDTNPA
                     VFPGAIELLNGIDDDCDFLADEGLVVQNGSFPNIEIFPNPTNNIFNIGLPSGTFVTIS
                     ISDINGALIFDQVECSGNTNIFDLTDQPAGIYWVKIICKGWIRMLSVEKI"
BASE COUNT          656 a          515 c          523 g          686 t
ORIGIN      
        1 aaaaacaggt ttttgcctgc tatatttttg taaggtgaaa aaaggttttc gccatattac
       61 ttcagttata gcctttgtag ttattgcatt tgcaagcacc attttgcaag cgcagaccta
      121 taatattgct acgtataatg gcatgaccgt aaccacctgt tcagggaatt tttatgatag
      181 cggtggaagc ggaggcaact atggcaacaa tgagaattat acagttacct tttgctccgc
      241 atcaggtagc ccgctctatt tcgatttttc ttcaagctct tcggcgttga acctggatgc
      301 cggaacgggc gatacacttt tcttctacga tggccttacc acttccgatc ctcttatcgc
      361 aaccctggta aaggctgatg actatgcttt cagttatccc gctgtaggca ctttcagttc
      421 ctgtgttacc attaaatgga aatccaatgc ttctgccgtt gatggcggat ggcaagcctc
      481 gatatcatgc gaagaaccgc caacctgtgc cgataatcct ccggctgcag atgtattcgg
      541 ccaggctact gcggtgtgta atctgaatga ttattgcggc accacttcca gatattacgg
      601 agaagatgca ccatttaatt tcggcggaac aggaggttct tgtcccggtc ctgatgatgg
      661 tttatttgca ggtaccctcg aaaataattc atggctgaaa tttattgcag acgctacttc
      721 ggcatcattc gatttcaatg ttaccggtgg cgcttcctgc gatggcgtgc aggttggtat
      781 tttttctttc gatgctgcta caaatacttt cgcaagaaaa tcgccttgca attataccga
      841 tggcggatgg tccggtgcat tcacgctgac agcaacagga ctcaccgttg gacaaacata
      901 ttatatgatg atcgatggta atgccgggtc gaattgcgat tataccatag gcgcaggaac
      961 aggtgtagca accgtaagcg ccggtgcgga tcagatcgtg tgtgcgttca ataccacact
     1021 cgctgcaaca acggcaatgg gctccggaac ctggtcggtt gtgagcggaa caggggtgtt
     1081 tgcagatgca acaaatccaa ctacctatgt ttccggattg tcgtatggta tcaatgtatt
     1141 cagatggaca tccaccggcg gattgtgtat ggacgttggc gcgtatgatg atgtggtggt
     1201 ggaaaatacg tgtttgctgc ctatcaccct ttcagatttt acggcaaccg tggtggatga
     1261 tatggtgcgg ctcaattgga ccaccgcatc tgagatcgac aatgatcatt ttgtgcttga
     1321 acgagccggt gctgatcagc agttcatggc catcgcagag atcatgggaa gtggcaccac
     1381 taccattccg gtgaactaca cttatactga tgatatgcca ctgccggcgg taaattatta
     1441 tcgcttgaag caggtggatg tcaatgccaa tacatcaaca agcaacgtta ttgcaattga
     1501 atcacattca gatgcacaaa ccttcactat cggcgattgc tacttcgaca atgcatcaga
     1561 ggtgctgacc attccatatc agacctcggc atctgttgca ttggacattc aattgtgcga
     1621 catcgccgga agaattttat tttctgctac atgccatacc acggcagtaa atgataatgc
     1681 gcaagtagac ctgcatcacc tgacccaaac aggtttgata ctcttgcaaa ttaccggtga
     1741 taatggcgtg cgctccaatc atactttttg gatcagtgaa tgatgtgggt tgaattttat
     1801 aaaaaattca taaagcaact ctacattaat tcaatgttta ccataatggc ggtaaaagtg
     1861 cacctcacca gggcaaagta taatgaaaaa ttatactaat ttattcatat ttggatgaat
     1921 tatatctttt cgacagaaag catacgaatc catcctttgc atataatttt aacccaatat
     1981 attcctgcag gttgatcggt caaatcgaag atattagtat ttcctgagca ttcaacttga
     2041 tcaaaaatta aggcaccatt gatatcgcta atagaaattg ttacaaaagt cccggaaggc
     2101 aatccaatat taaaaatatt gtttgtggga ttaggaaata tttcaatatt cggaaaagat
     2161 ccattctgca ctactaatcc ttcgtcagct aaaaaatcac aatcgtcgtc tattccattg
     2221 agcaactcga ttgcgcctgg aaatacagca ggattggtgt cgttgcagtc tgtagaatct
     2281 agaacatatc cggagatttc catgcaggtc aaggtgtcta tattcgcatt accaaataaa
     2341 tcaccatctg catcctgata taaccatgtg aaagtcaaat
//
LOCUS       DAOQOU010000282         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_39262_length_2331_cov_4.806678, whole genome shotgun sequence.
ACCESSION   DAOQOU010000282 DAOQOU010000000
VERSION     DAOQOU010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39262_length_2331_cov_4.806678"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLM90_14440"
     CDS             complement(<1..796)
                     /locus_tag="PLM90_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90632.1"
                     /translation="MKTIQLLLACMLFTHGVFAQVTTEKSIEFEEIEGYDGFSIEPFG
                     KNGLLVYSKSEEGNSYRIQRYNTDLEPEEKKDITLPKGYFMIGEYLDDESINFLFSNK
                     KGQFIFYQVNGNTFDVSKASGIFPKKTYQGFFVAKGNFAYITRSNKKGVTVIQLNIST
                     GKSKIIPLNISPYTLKDLTVEKIQPIAESNETFIYVNAYNKKDHNLYVMQVNESGTVT
                     NTFNLSKDTEKKLSSVSASPIGDNEYVFTGTYSSRSSATSEGMYICS"
     gene            877..2112
                     /locus_tag="PLM90_14445"
     CDS             877..2112
                     /locus_tag="PLM90_14445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ90633.1"
                     /translation="MSIDFSAFDFAPALSEGLDAMGFRNPTPIQEQAIPFILSGNDLI
                     ACAQTGTGKTAAFLLPVMHKIISGNSDGVNTLIIVPTRELANQIDEAIRGIAYFTSLS
                     SIAVYGGNDGIMFEQEKRAFREGVDIIVGTPGRLLSHIQQGYLKFDTLKHLILDEADR
                     MLDMGFSDDIAKIISNIPHHPQTLLFSATMPHKIREMAKRILREPQQINIALSKPAAG
                     VSQGVYIVHDAQKMPLLVDILSLKQLKSMLVFASTKDKVKQLELELKRRKFNVAAIHS
                     DLEQAVRKSVLRSYANREIQILVATDILSRGIDVDEIGLVINYDVPGDAEDYVHRVGR
                     TARAESTGEAITFVGPADQSKFSRIEQLIGDTIPRLEIPESLGPGPEYNPRAHHGHAK
                     SGGRPNQRRRRPNGGNRRH"
     gene            complement(2113..>2331)
                     /locus_tag="PLM90_14450"
     CDS             complement(2113..>2331)
                     /locus_tag="PLM90_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90634.1"
                     /translation="ELSEMSVSLINGTDTVLLWPDATVNYGNIKSYTINNWDIDIWNV
                     NADATIQFKAVDKHDNSSTADVPVQLTM"
BASE COUNT          673 a          465 c          475 g          718 t
ORIGIN      
        1 cactgcagat atacatgcct tcagaggttg cggaactacg tgaagaatag gtgccggtga
       61 acacatattc attatcgcca atgggactag ctgacacgga tgataatttt ttctcggtgt
      121 ctttagataa attgaatgtg tttgtcaccg tgccggattc attcacttgc attacataaa
      181 ggttgtggtc ctttttattg taagcattta cgtaaataaa ggtttcattc gattctgcaa
      241 tcggctgaat tttttcaacg gtcaggtctt ttaaagtata tggcgaaata ttcaatggta
      301 tgattttgct tttaccggtg ctgatgttta attgtataac ggtaacacct ttcttattgc
      361 ttcgggtgat ataagcaaaa tttcccttgg caacaaaaaa accttgatac gttttctttg
      421 gaaaaatacc ggatgctttt gaaacatcaa aggtgtttcc attcacctga taaaaaatga
      481 attgcccttt tttatttgaa aacaaaaaat tgatcgattc gtcatccagg tattcgccga
      541 tcataaaata tcccttgggt aaagtgatat ccttcttttc ttccggttcc aggtcggtgt
      601 tatatcgttg tatccgataa ctatttcctt cctctgattt gctgtaaacc agtaaaccgt
      661 ttttgccaaa tggttctatg gaaaaaccat cgtagccttc tatttcttca aattcaatac
      721 ttttttctgt agtcacttgt gcaaaaacac cgtgtgtaaa tagcatgcat gcaagtaata
      781 attgtattgt tttcataatg tggctgtttg aaatttactt caaatatacc tataagcgtt
      841 tagtaggcaa atgataattc cgtatctttg attattgtga gtattgattt ttctgctttt
      901 gatttcgctc ccgctctttc tgaaggttta gacgccatgg gatttcgcaa tcctactcct
      961 atccaggagc aggcaatacc cttcatttta agcggtaatg accttattgc atgcgcacaa
     1021 accggcaccg gaaaaacggc tgcattttta ttgcctgtca tgcataagat catcagtgga
     1081 aattcagatg gtgtgaatac gctgatcatc gttccaacca gagaacttgc caaccagata
     1141 gatgaagcca tcagaggtat agcatatttt acttctttga gttccattgc tgtatatggc
     1201 ggcaacgatg gtattatgtt tgaacaggag aagcgcgcct tccgagaagg tgtcgatatt
     1261 attgttggca cacccggcag attgctctcg catatccaac aaggatacct gaagtttgat
     1321 acactgaaac acctgatact ggatgaagca gatcgcatgc tggatatggg attttctgat
     1381 gatatcgcaa aaatcatttc caatatcccg catcatccgc aaacattatt gttttctgcc
     1441 accatgcctc acaagattcg tgaaatggca aaacgcattt tgcgcgaacc tcaacaaatc
     1501 aatattgccc tttctaaacc ggcagccggt gtatctcaag gtgtatatat cgtgcacgat
     1561 gcacaaaaaa tgcccttgct tgtagatatt ttatcgctaa agcaattgaa gtcaatgttg
     1621 gtatttgctt caacgaaaga taaagtaaag caacttgagc ttgaattaaa acgtaggaaa
     1681 tttaatgtag ctgcgataca ctccgacctg gagcaggctg ttagaaaaag tgttttgcgt
     1741 agctatgcca atagagaaat tcaaatttta gtagccaccg atattttgtc gcgcggaatt
     1801 gatgttgatg aaataggatt agtaattaat tatgatgttc ccggtgatgc tgaagattac
     1861 gtgcacaggg tgggcagaac tgccagagca gagagcaccg gtgaagccat tacttttgtg
     1921 ggtccagcag atcaatcaaa attttcgcgc atagaacaac tcattggtga caccattcct
     1981 cgcttggaaa tccctgaatc tttaggtccc ggacctgaat ataacccacg tgcacaccat
     2041 ggacatgcca aatcaggcgg cagaccgaat caaaggcgtc gccgaccaaa tggtggtaat
     2101 cgaaggcact aattacatcg taagctgaac aggtacgtct gcagtgctgg aattgtcgtg
     2161 cttgtcaaca gctttaaact gaatagttgc atcagcattc acattccaga tatctatatc
     2221 ccaattattg atagtatagg atttgatatt accgtagttc acagtagcat caggccaaag
     2281 tagaacggtg tcagtcccat tgatgagaga taccgacatt tcagataatt c
//
LOCUS       DAOQOU010000283         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_39322_length_2329_cov_6.202287, whole genome shotgun sequence.
ACCESSION   DAOQOU010000283 DAOQOU010000000
VERSION     DAOQOU010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39322_length_2329_cov_6.202287"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLM90_14455"
     CDS             complement(<1..365)
                     /locus_tag="PLM90_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMZ90635.1"
                     /translation="MDLTIGLISEIEKKWQSEWRKSDLYKVTEDPSRPKYYVLDMFPY
                     PSGSGLHVGHPLGYVASDIISRYKRLKGFNVLHPMGFDAFGLPAEQYAIETGQHPAVT
                     TEKNIAYFREQLDRMGFCY"
     gene            485..1366
                     /locus_tag="PLM90_14460"
     CDS             485..1366
                     /locus_tag="PLM90_14460"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HMZ90636.1"
                     /translation="MSQQAGKSLQRKQTHYVNAILITALALVMLGVMSILMISFKYEE
                     ANIKEDLVISAYLKDGLTQTDVDKLIKRITAEPSVHDTHYISKEAAMDIFKEKFNEDP
                     GEILEDVNPLPASIDISLDAAAVNVDSINAFRERLQLYPEITLTRANEALISSVDTDL
                     KVAGFVIAGLSILFLIIAIAIIDKTIRLSMYSNRFLVRSMQLVGATRGFITGPYVRRS
                     IINGVISAFIAAFILMLGVIYVHNNYNYWDFSNPTIKRGFMFTMLMLLAVGVLITWFS
                     TRSSVHKYIKMKLDELY"
     gene            1386..1664
                     /locus_tag="PLM90_14465"
     CDS             1386..1664
                     /locus_tag="PLM90_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HMZ90637.1"
                     /translation="MDNSTSPKKETPKTTSPAGPMMVLGKQNYYLILFGLAVIIIGFI
                     LMSGGKSPDPNVFNADEVYSTRRIVVAPIVVILGFAIEIYAVFHKSKK"
     gene            1670..>2329
                     /locus_tag="PLM90_14470"
     CDS             1670..>2329
                     /locus_tag="PLM90_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMZ90638.1"
                     /translation="MTIFEAVVLAIVEGITEFLPVSSTGHMIIASSLMGIEADDFTKA
                     FTISVQFGAILSVLALYWKRFLRSVNFYVKLFVAFIPAVVVGVLLGDWIDSLLENVVA
                     VAIALILGGIVLVFIDRLFSADKADDITDPENIPYKNAFAIGVFQCIAVIPGVSRSAA
                     TIIGGLTQKLTRKAAAEFSFFLAIPTMAGAMFKKMVMDEIESGANKGLHPYELLLNNH
                     DN"
BASE COUNT          658 a          471 c          499 g          701 t
ORIGIN      
        1 tcgtaacaaa atcccatgcg atcgagctgc tcgcggaaat aggcaatatt tttctcggta
       61 gtaactgccg gatgctgacc tgtttcaatg gcatactgct cggcgggaag tccgaaggca
      121 tcgaatccca tagggtgcaa tacgttgaaa ccctttaagc gcttatatct ggagataata
      181 tcactggcta cataaccgag gggatgacca acgtgcaagc cacttccgct gggatacggg
      241 aacatatcca acacataata tttcgggcgc gaagggtctt cggtaacctt gtataaatca
      301 ctttttcgcc actccgattg ccatttcttc tcgatctcac tgatcagtcc aatcgttaaa
      361 tccatgcctc aaagttagtg ttttcgggct attcgagggt tattggctat ggattgcagt
      421 tacccacatg caagtgtaga tgaacaaagg ccggaatgag gtcaaagacg tatttttgca
      481 gcagatgagt caacaggccg ggaaatcgct gcaacgtaag caaacccact atgtcaacgc
      541 catactgatt acggcactgg cattagtgat gttgggtgta atgagcattc tgatgatcag
      601 cttcaaatac gaagaagcaa atatcaaaga agacctcgtt atttcggctt atttaaagga
      661 tggtctcaca caaaccgatg tcgacaaatt gataaagcgc atcactgcgg aaccttcggt
      721 gcatgacacg cattatattt ccaaagaagc ggcgatggat atcttcaaag aaaaattcaa
      781 tgaagatccc ggagaaattc ttgaagatgt aaatccatta ccggcatcaa ttgatatcag
      841 tctggatgct gctgccgtaa atgttgattc tataaatgca tttcgcgaac gtttacaatt
      901 atatccggag attacattaa cacgtgcgaa cgaggcatta atctcaagtg tagataccga
      961 tttgaaagta gccggatttg tgattgcagg cttatcaatt ttattcctca tcattgctat
     1021 tgccattatc gataaaacaa tacgtttaag catgtattcg aatcgttttt tggtgcgcag
     1081 tatgcaattg gttggcgcaa cgcgtggatt tataaccgga ccttatgttc gccgaagcat
     1141 tattaatggt gtgatatctg cttttatcgc cgcatttatt ttaatgctcg gtgtaattta
     1201 cgtgcacaat aattataatt attgggactt cagcaatccc accatcaaac gtggctttat
     1261 gtttaccatg ttgatgttac ttgctgtagg tgtgctgatc acctggttta gcaccagaag
     1321 ttcagtgcat aaatatatta aaatgaaact cgacgaactt tattaatcat ccaacacttc
     1381 aggatatgga caactcgaca tcgccaaaaa aagaaacccc taaaaccaca tcgcccgccg
     1441 gtccaatgat ggtgcttggg aaacagaatt attatttgat cctgttcggt cttgcagtaa
     1501 tcattattgg atttatttta atgagtggcg gaaaaagccc tgatccaaat gtgttcaatg
     1561 ccgatgaagt atacagcacc cgtcgcattg ttgtggcgcc tatcgtagtt attctcggtt
     1621 ttgctatcga gatctacgct gtatttcata agtctaaaaa ataataagca tgaccatttt
     1681 cgaagcggtg gtcctcgcca ttgttgaagg catcaccgaa tttcttcccg tttcatctac
     1741 gggtcatatg atcattgctt cctcattaat gggtatagaa gctgatgatt ttacgaaggc
     1801 atttaccatt tcagttcaat ttggagcaat actctcggta cttgctttgt attggaaacg
     1861 ttttttgcga tccgtgaatt tctatgttaa attatttgtc gcctttatcc cggctgttgt
     1921 agttggtgtg ctgttgggcg attggataga tagtttgctg gagaatgttg tagccgtagc
     1981 cattgcactt atccttggtg gaattgtatt ggtatttata gatagattat ttagtgccga
     2041 caaagccgat gacattaccg atcctgaaaa cattccttat aaaaatgcat ttgctatagg
     2101 tgtatttcaa tgcatagccg ttattcccgg tgtatccaga tcggcagcaa caattattgg
     2161 cggactcacg caaaaattga cacgcaaagc cgctgcagag ttttcgtttt tccttgctat
     2221 tcccacaatg gcaggcgcta tgtttaaaaa aatggtgatg gatgaaattg aaagcggtgc
     2281 aaataaagga ttgcatccat atgaattgct actcaacaac cacgataat
//
LOCUS       DAOQOU010000284         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_39456_length_2325_cov_5.090749, whole genome shotgun sequence.
ACCESSION   DAOQOU010000284 DAOQOU010000000
VERSION     DAOQOU010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39456_length_2325_cov_5.090749"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /gene="serS"
                     /locus_tag="PLM90_14475"
     CDS             <1..739
                     /gene="serS"
                     /locus_tag="PLM90_14475"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMZ90639.1"
                     /translation="LTQYFLDQAAAAGYMEVVPPFMVNADSGYGTGQLPDKEGQMYHA
                     TEDDFYLIPTAEVPVTNIYRDTIVKEEDLPIKMTAYSPCFRREAGSYGKDVRGLNRLH
                     QFEKVEIVEIAHPNTSAERLDVMVAHVEKLVQSLELPYRILRLCGGDMGFASAITYDF
                     EVWSGAQQRWLEVSSVSNFESFQANRMKCRFKDSSDGKNKLVHTLNGSALALPRILAS
                     ILENNQTEGGIDIPKVLVPYTGFDKIS"
     gene            739..1617
                     /locus_tag="PLM90_14480"
     CDS             739..1617
                     /locus_tag="PLM90_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90640.1"
                     /translation="MVQRLLIACLVLAGIIGCKHRKDPGPLTDGTETLIENDVYVVHS
                     MIYADEKPWTTSNIFVFKGLGDTIWIYGSGYGDFVDPCGDCNDNNFYLGDNMHGTGPA
                     TVDVEQVDSVITKIFGRNRDSVLLQFIVPHYHNDHINNEFVDAFHSSFRYPLRSDEKI
                     WIHTNDYYGATCNEPCCGTQPCPDKKHNKYFGVPYLPAWKPEYLALFTKMGNENDDCD
                     TALKSFTTTSGSWTITKAMSTADGGHTDGTVNIKSFDKKILIAGTKSKIQCPYPDDWQ
                     FVSVHGNIVGATTGNE"
     gene            complement(1620..>2325)
                     /locus_tag="PLM90_14485"
     CDS             complement(1620..>2325)
                     /locus_tag="PLM90_14485"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ90641.1"
                     /translation="RDTHPEIELYIGDGSHPSAAGSYAAACTFYAAIFRNDPTLITYD
                     FTLPADQAHIIREATKLVVYDSLASWYIGTYIHEPIANFNVNTGIPFTASFENLSENA
                     ISYLWNFGDGTNSTEAAPVHIYGAEGMYIVSLEVTGCSDVDDYTSDITITGVETSIQN
                     NAIINWQPENPVHAELFLPANNSNWRIEILDMAGNVVMQTYAQTLITVDGIHAGAYII
                     YLQQGDERMYRKLMIQ"
BASE COUNT          675 a          511 c          486 g          653 t
ORIGIN      
        1 tttgacccaa tatttcctgg atcaggcagc agcagcgggt tatatggaag ttgtgcctcc
       61 tttcatggta aatgccgatt cgggatacgg caccggacag cttcctgata aagagggcca
      121 aatgtaccat gccaccgaag acgacttcta tctcataccc accgccgaag tgccagtaac
      181 taatatctat cgcgatacta tcgtaaaaga agaggacctt cctataaaaa tgaccgccta
      241 cagcccttgt ttccgtcgag aagcgggtag ctatgggaaa gatgtacgcg gtttaaatcg
      301 cctgcatcag ttcgaaaagg tggaaatcgt ggaaatcgca catcccaata cttctgccga
      361 acgtcttgat gtcatggttg cccacgtgga aaaactcgtg caatcgctcg agctgcctta
      421 tcgcattctg cgactttgtg gtggcgatat gggctttgct tctgctatca catacgactt
      481 cgaagtatgg tccggcgctc aacaacgctg gctggaagtg agctctgttt ccaattttga
      541 atccttccag gctaatagga tgaaatgtcg tttcaaggat agcagcgatg gtaaaaataa
      601 actcgtacat acacttaatg gctcagcact cgccttacca cgtattttgg cgtctattct
      661 tgaaaataat caaacagaag gcggaattga tattccaaaa gtattggtgc cttacaccgg
      721 ttttgataaa atttcctgat ggttcaacgc cttctcatcg cttgcctggt gctggcaggt
      781 attatcggat gtaagcacag aaaagatccg ggaccgctta ccgatggcac cgaaacactc
      841 attgaaaatg atgtctatgt tgtacacagc atgatctatg ctgatgaaaa accctggacc
      901 acatccaata tttttgtttt caagggcctt ggcgatacca tttggatata tggttccggt
      961 tatggcgatt ttgttgatcc ttgcggggat tgcaacgaca ataattttta tctcggcgac
     1021 aatatgcacg gaacaggacc cgcaactgtt gatgttgaac aggtagattc agtaatcaca
     1081 aaaatatttg gtcgcaatcg cgatagtgta ttattacaat ttattgtacc acattatcac
     1141 aacgatcata ttaataatga atttgttgac gccttccatt cttcttttcg ttatccattg
     1201 cgctcagatg aaaaaatttg gatacatacc aacgactatt acggcgctac atgcaacgaa
     1261 ccttgctgtg gcacgcaacc ctgtcctgat aaaaaacaca ataaatattt cggagtgccg
     1321 tatttaccgg catggaaacc ggaatatctc gcgcttttta caaaaatggg taatgaaaac
     1381 gacgattgcg atactgcatt aaaatcgttt acgaccactt caggcagctg gactatcacc
     1441 aaagcaatgt caaccgctga tggcggacat accgatggca cagtgaatat taaaagcttc
     1501 gataaaaaaa tattaatagc cggaacaaaa agcaaaattc aatgcccata tcccgatgac
     1561 tggcaatttg taagcgtgca tgggaatatt gttggcgcta ctacgggtaa tgaataatat
     1621 tattgaatca tgagtttgcg atacatacgc tcgtcgccct gttgtaaata aatgatataa
     1681 gcaccggcgt gaatgccatc caccgtaatt aatgtttgtg cataggtttg cattaccaca
     1741 ttgccggcca tatccaatat ctcaatgcgc caattgctat tatttgccgg taaaaataat
     1801 tcagcatgca ccggattttc gggttgccag tttattatcg cattattctg aatagaagtt
     1861 tctacgccgg taattgtaat atcgcttgta taatcatcaa cgtctgaaca ccctgtaact
     1921 tctaaactta cgatatacat accctctgct ccataaatat gtaccggcgc cgcctctgtt
     1981 gaatttgtac cgtcaccaaa attccataaa taactaatgg cattctccga cagattttcg
     2041 aaacttgcgg taaaggggat accggtattc acattaaaat ttgcaattgg ctcatgtata
     2101 tatgtgccta tataccatga agcaagacta tcgtaaacca ccaacttcgt tgcctcgcga
     2161 ataatatgtg cctgatcagc agggagcgta aaatcatatg tgatcaatgt tgggtcgttt
     2221 ctaaaaatgg ctgcgtaaaa tgtacatgct gctgcataag atcctgctgc ggaagggtga
     2281 ctgccatcgc caatatataa ttcgatttca ggatgtgtat cgcgc
//
LOCUS       DAOQOU010000285         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_40345_length_2296_cov_5.561803, whole genome shotgun sequence.
ACCESSION   DAOQOU010000285 DAOQOU010000000
VERSION     DAOQOU010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40345_length_2296_cov_5.561803"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..483)
                     /locus_tag="PLM90_14490"
     CDS             complement(139..483)
                     /locus_tag="PLM90_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90642.1"
                     /translation="MKTLATLIILFSTTLVFAGNDKNISDREKATVINNVTQIVTNQD
                     VVSALGVSGQADVTLAVDEQGVIHVAEVQANNFLLEYHIRQSIEGAKMIVEDSLVGKT
                     INFFMNVVQPNE"
     gene            complement(593..1831)
                     /locus_tag="PLM90_14495"
     CDS             complement(593..1831)
                     /locus_tag="PLM90_14495"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ90643.1"
                     /translation="MEPEAQQVKSSIGHRIYFVLICGIALLIPFHNILPSPMLILTFL
                     SILITGEYATKWKRITSNKRFLLFAAIYIPAVYGYFLSSNTTIAGHDLQVRLYALLIP
                     IFFTLAGPLSKKELRRLLSCYVISSVLFALTAIVVAIWRYPDIGAQAFYYKELVSFTF
                     IHPSYAAMFLVFAVMLVSAELVTRWEEITSGNRIIYILLMLLLSLIVLLLTAKLAIAC
                     LFIVISLAVFVWGKKNYNTTKAILILISLNIAMFIAMMMLPYTRERMQMLLHYKETSY
                     SNSVDSREQIWHSAIQVASDHLIVGIGTGDAEDMLVSQYAANQFETGVQERYNCHNEY
                     LQVLVDTGLPGLIFFLLVLTGCFRLAFIDKNYLWMAFLLLFAINIITENMLRTQSGIV
                     FFSFFNALLGLHSGRKQAGV"
     gene            complement(1831..>2296)
                     /locus_tag="PLM90_14500"
     CDS             complement(1831..>2296)
                     /locus_tag="PLM90_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HMZ90644.1"
                     /translation="FYSQDRVGENNKPFSIIKFRTMIVDAEKHTGPKFADRNDPRITR
                     IGNILRKTRLDELPQCWNILRGDINLIGPRPERDFFVQELSKQIPFFSLRSAVKPGLT
                     GWAQVNYHYANNFDDTLVKLQYDMYYIKHRSLYLDMVIILKTIGVMIKFKGT"
BASE COUNT          726 a          472 c          435 g          663 t
ORIGIN      
        1 acttgatacc tgatacttga tacctgatac ttgatacttg atacctgata ccaatataat
       61 aaaaaaaacc ctccgtagag ggtcttgacg gtcaaagtgg atgacgttac tttaaccgta
      121 tattatccat cgtcatgatc attcgttagg ttgcactaca ttcatgaaga agttgatagt
      181 tttacctacc agcgagtctt ctacaatcat ttttgcacct tcaatacttt ggcggatatg
      241 gtattccaaa agaaagttat ttgcctgcac ttcagcaacg tgaatcacac cttgttcgtc
      301 aacagcaagt gttacatctg cctgaccgct tacaccaagt gcgcttacca cgtcttgatt
      361 cgtaacaatt tgcgttacat tattaattac agttgctttt tcgcgatcgc tgatattttt
      421 atcgtttcct gcgaatacaa gtgttgtgct gaagaggatg atgagtgtag ctaaggtttt
      481 cataatattc tgtttgtata gtttgtttga tgttgttttg ataaatacga acatatggcg
      541 aactgccccc ataaaaagtt acagaagccg caaaaaaaat tgttaaaaag tgctaaacgc
      601 ccgcctgttt tcgcccggaa tgcagtccca gcaaggcatt gaagaaacta aaaaaaacga
      661 tgccactctg agttcggagc atgttttcgg tgatgatatt gatggcaaac aacaaaagaa
      721 aggccatcca gaggtagttt ttatcgataa aagccaatcg aaaacagcct gttaatacca
      781 gtagaaagaa gattaatcca ggcaggccgg tgtctacgag gacctgcaga tattcattat
      841 gacaattata tcgctcttga acaccggttt caaattgatt cgcagcatac tgtgatacta
      901 acatgtcttc agcatcgcct gtacctatgc ctacgatcaa atgatcggat gcaacctgaa
      961 tagcggagtg ccaaatttgt tcacgggaat cgacactatt actatagctg gtctctttgt
     1021 aatgtaatag catctgcatg cgttctcgcg tgtaaggcaa catcatcata gcaataaaca
     1081 tcgcaatgtt cagacttatt aaaatcaata ttgcctttgt cgtattataa tttttcttcc
     1141 cccaaacaaa aactgccaga gaaattacta taaataagca tgctatcgcc agttttgcag
     1201 taagcagcaa tacaatcagg gaaagcaata acattaatag tatataaatt atgcgattgc
     1261 ctgaggtaat ttcttcccac ctagtaacca attccgccga caccaacatc actgcaaaaa
     1321 caagaaacat tgctgcatat gacggatgaa taaatgtgaa cgatacaagt tctttataat
     1381 aaaatgcctg tgcgccgata tccggatatc gccatatagc aactactata gctgttaatg
     1441 caaacaatac acttgaaata acataacatg ataataatcg tcgcaattct tttttcgaaa
     1501 gcggaccggc gagggtgaaa aatataggta tcagtaatgc atacaaacgc acttgcaggt
     1561 catgcccggc gattgttgta tttgaagaaa gaaaatatcc ataaacagca ggaatataaa
     1621 ttgccgcgaa taacaagaaa cgtttattgc ttgtaatcct tttccactta gtggcatact
     1681 caccggtgat caaaatcgac agaaacgtca atatcaacat tggcgatggc aggatattat
     1741 gaaatggaat taataatgca ataccgcata tcagcacaaa ataaatgcga tgaccgatag
     1801 aggattttac ttgttgcgct tcaggctcca tcaggtacct ttgaatttga tcatcacgcc
     1861 aatagttttc agaatgatca ccatatccag atataggctg cgatgtttga tatagtacat
     1921 atcatattgc aatttcacga gtgtatcatc gaagttgttg gcataatgat agttcacctg
     1981 tgcccaaccg gtaagtccgg gttttactgc cgatcgcagc gagaaaaatg gaatctgctt
     2041 actcaattcc tgcacaaaaa aatcgcgttc cggtcttggg cctatcaggt tgatgtcgcc
     2101 gcgcaaaata ttccagcatt gcggcaattc atccaaacgc gtttttctca gaatattgcc
     2161 aattcgtgta atacgaggat cattgcgatc ggcaaatttg ggacccgtat gtttttcagc
     2221 atccacaatc atggtgcgga acttaatgat gctaaatggt ttattattct cccctactct
     2281 atcctgagaa taaaat
//
LOCUS       DAOQOU010000286         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_40969_length_2276_cov_6.782981, whole genome shotgun sequence.
ACCESSION   DAOQOU010000286 DAOQOU010000000
VERSION     DAOQOU010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40969_length_2276_cov_6.782981"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1218
                     /gene="pheS"
                     /locus_tag="PLM90_14505"
     CDS             166..1218
                     /gene="pheS"
                     /locus_tag="PLM90_14505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMZ90645.1"
                     /translation="MDAKGDQFDHIKEIQEDINSFPVTDAASLEQFRIRFLGSKNILK
                     DVFSAIKDVPNERKKEFGQLVNALKQSAEQRFESLEAQYKKQSDKSADQVDVSRPAIT
                     NAPGSLHPVTLIKDELIGIFTRMGYTVVDGPEIEDDWHNFSALGMPEDHPARDMQDTF
                     YISTNPAVLLRTHTSNVQIRVMEELAKSGKQPPVRIVALGRTYRNETISARAHCTFNQ
                     IELLYIDEGVTFADMKQTLLYLAQELYGENTTIRLRPSYFPFTEPSAEMDVSCFVCGG
                     DGCAICKHSGWVEIGGCGMVDPVVLENCGIDSEKYTGFAFGFGLERIAILKYKINDIR
                     LFTENDLRFLKQFEQL"
     gene            1227..1832
                     /locus_tag="PLM90_14510"
     CDS             1227..1832
                     /locus_tag="PLM90_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90646.1"
                     /translation="MKIILSLFAFICLLQVDMHAQNTTLGADTSKITKDSIIMTIVGE
                     DTLIGPPITFRKYNERFVDLMFFDGNYNIVSFHIAEGSTSFQLMLSSLDSATYCFSVS
                     PDEFKMLMAYMKQFNDELLSQPNYPVKCNYCQTIQVFWYDGDKEKSTLKRSYRIPQVF
                     PEILDFCYDLSDDKRRCKISLDNFMSLEGFEDNLRGPASQK"
     gene            1837..>2276
                     /locus_tag="PLM90_14515"
     CDS             1837..>2276
                     /locus_tag="PLM90_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ90647.1"
                     /translation="MPFSYTDSLNYVSKLTPRRVWNMTKILGSYYYSMWTKKPVHKGN
                     PISISFEPTTSCNLRCPECPSGLRAFTRPTGMLNQDFFRDTIDAIHKDLTYLIFYFQG
                     EPYLNPAFLDMVAYANKKKIYTATSTNAHYLNDENARKTIESGL"
BASE COUNT          665 a          481 c          481 g          649 t
ORIGIN      
        1 tagtaagact aatgcagtgg aatgtgccgt gaattttctc tgtgcacctc tgtgttccgc
       61 taagtggcct ctatggttaa atggtgagtg gggctaattc acgattcacg agtcacgact
      121 caccactcac cactcacctt tacgtattct tcgtattttt gccccatgga cgcgaaaggt
      181 gaccaattcg atcatataaa agaaattcag gaagacatca acagcttccc tgttacagat
      241 gctgcttcgc tcgagcagtt cagaattcgt tttttgggaa gtaagaatat cctgaaggat
      301 gtattttccg ctattaaaga tgttcctaac gaaaggaaaa aagaattcgg acaattggtg
      361 aatgcactta aacaaagtgc agaacagcgt ttcgaaagcc tcgaagctca atacaaaaag
      421 caatctgaca agagcgccga tcaggtggat gtttcacgac cggcgataac aaacgctccc
      481 ggttcactac atcctgttac acttataaaa gatgaattaa tcggcatctt cacacgaatg
      541 ggctatacag tggttgatgg tccggaaatt gaagacgatt ggcataattt cagcgcactg
      601 ggtatgcccg aagatcatcc tgcccgcgat atgcaggata ctttttatat cagcacaaac
      661 cctgccgtat tattgcgcac ccatacttcc aatgtgcaga ttcgtgtaat ggaagaactc
      721 gccaaatcag gcaaacaacc accggtgcgc atcgttgctc tcggacgaac atatcgcaat
      781 gaaacaattt cggctcgcgc acattgtacg tttaatcaaa tagaattatt gtatatcgat
      841 gaaggcgtca cctttgccga tatgaaacaa actttgttat atctggcgca agaattgtat
      901 ggagaaaata ctaccatccg cctgcgacca agttatttcc cctttacgga accaagtgct
      961 gaaatggatg ttagttgctt cgtctgcggt ggcgacggct gcgcgatctg taaacatagc
     1021 ggctgggtgg aaattggcgg ttgcggtatg gtggatccgg tggtactgga aaactgtggc
     1081 atcgactcgg aaaaatatac cggcttcgct tttggattcg gtctcgaacg catcgctatt
     1141 ttgaaataca agatcaacga tattcgttta ttcaccgaaa atgacctcag atttttgaag
     1201 cagttcgaac agctttgatg aacgatatga aaataattct cagcctattc gcttttattt
     1261 gcctgctgca ggtagacatg catgcacaaa acactacact tggtgccgat acctctaaaa
     1321 tcactaagga tagtatcatc atgaccatcg tgggtgagga tacccttatc ggtccgccca
     1381 taacttttcg aaagtataat gaacgatttg tcgacctgat gttttttgat ggtaactata
     1441 atatcgtgag ctttcatatc gctgaaggaa gtacttcatt ccaactcatg ctcagtagcc
     1501 ttgattcagc cacttattgt ttttcggttt cccccgacga gtttaaaatg ctcatggcat
     1561 acatgaagca atttaatgat gaattattgt cgcagccgaa ttatcccgtg aagtgtaatt
     1621 actgtcagac aattcaggta ttttggtacg atggcgataa agaaaaaagt actttgaaaa
     1681 gatcttatcg cataccacaa gtgttccccg aaatattaga tttttgctac gatctgagtg
     1741 atgataaacg cagatgtaaa ataagcttag ataatttcat gtcgctggaa gggtttgagg
     1801 ataacctccg aggacctgct agccaaaaat aaaatcatgc catttagcta caccgactcg
     1861 ctgaattatg tcagtaagct gactccgcgc cgtgtgtgga atatgactaa aattttgggt
     1921 tcatactatt atagtatgtg gactaaaaag cctgttcaca agggcaatcc gatttctatt
     1981 tcttttgagc ctactacttc ttgtaatctc cgttgcccgg aatgcccaag tggattgcgt
     2041 gcatttacca gaccaacagg tatgctgaat caggattttt ttagagatac tattgatgct
     2101 atccataaag acctgacata cctcatattt tattttcaag gtgagccgta tttgaatcct
     2161 gcctttctcg acatggttgc atatgccaac aagaaaaaga tttataccgc aacatctaca
     2221 aacgcgcatt atctcaatga tgaaaatgcg cgaaaaacta ttgaaagtgg tcttga
//
LOCUS       DAOQOU010000287         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_42424_length_2232_cov_5.599449, whole genome shotgun sequence.
ACCESSION   DAOQOU010000287 DAOQOU010000000
VERSION     DAOQOU010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42424_length_2232_cov_5.599449"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2232
                     /locus_tag="PLM90_14520"
     CDS             <1..>2232
                     /locus_tag="PLM90_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90648.1"
                     /translation="FGRIDIITVSSIPKPINHDLYGINIEGLFKQHTLPYDGLNGGSS
                     EYAYDWLAELAPRVIRFPSGESSKFMHLMHNTNGTNSVGYGYDIKEIARYFDQTGGDK
                     TLAAAAAEDGITVNEERLVEDPEEMEDWMDVQFTGNYLDFLLKWKEQQCVDHAYIEDF
                     INLIHTIDAANPGRPKVHVILTLNVISETASECLAIADYLRANNVNVIGVEMGNETYA
                     DFYCKSVGFSDFTHYYDYLNGNNYTGGGYTTTESENLDKVLSDAMWYGGHDYLNTFQN
                     IASYNYKVGLVGKPIGGEFAFKAEDGGGGGTACSDDNWNYLLSGKYDETISGKYAFDA
                     VIMHTYYSADNWDHFVYDIYNFVLSNLTPCNPSPYPSIWDDLWHFGYADPDVRLQTMF
                     EKLRGNGYEEGETGNYMDFLSNHATTPVDLYKYSMLSSFKEFGNQLDLDRTDAGKKDL
                     WVTEWNNKTVITEHTTDHWPLDWPVGIDITNDLQQKMLEAPSNTFLSGYLLMRWWLKE
                     IEMNYYINDFRQNFFTISTMQGYGGGVWSDLLTLTTGDEREAEGTDIVPWEDIGGTIP
                     ETPGCDYIGESGKKFLERNYWMRRMNYYVARLFSDISRNHLLFVPSAFLYDEHNINLP
                     PMVFIDDDVNPQYIYIYFTNVKDVIQNYTLDPQFIRLMFTPDADDVILGIPQINYIIA
                     NELYATFGNTKLYNTSLNNCYDDYEQPTYLANAQMVWGDSYPPSCIGAIHPDHCFTAL
                     PYT"
BASE COUNT          701 a          382 c          485 g          664 t
ORIGIN      
        1 attttggcag aattgatatt attaccgttt caagtatacc caagccaatt aaccatgatt
       61 tatatggtat taatatcgaa ggattgttta aacagcatac tttgccctat gatggcctta
      121 acggtggttc aagtgagtat gcctatgact ggcttgcaga actggctccc cgtgttattc
      181 ggttcccaag tggcgaatca agtaaattta tgcacttaat gcataacaca aacggaacta
      241 atagtgtagg ttatggatat gatattaaag aaatagctcg atattttgac caaacagggg
      301 gtgataaaac tttggctgca gccgctgctg aagacgggat tacggttaat gaagaaaggc
      361 tagtcgagga ccctgaagag atggaagatt ggatggacgt gcaatttact ggaaattacc
      421 ttgattttct gctaaaatgg aaagagcaac aatgcgttga ccacgcatat attgaagatt
      481 ttatcaacct gatacataca atagatgcgg caaatcccgg caggcctaaa gtgcatgtaa
      541 ttttgacact caatgtgata agtgagacgg cttctgagtg tctggcaatt gccgactatt
      601 taagagccaa caatgtcaat gtaattggcg ttgaaatggg taatgaaaca tacgccgatt
      661 tttattgcaa aagtgttggt tttagcgatt ttacacatta ctatgattat ttaaatggaa
      721 ataattacac gggtggtggc tacaccacaa cagaatctga aaatttagac aaggtattat
      781 ctgatgccat gtggtacggt ggacatgatt acttaaatac atttcaaaac attgcgtcat
      841 acaactacaa agtgggatta gttggcaaac ccattggtgg tgagtttgcg tttaaagctg
      901 aagacggggg tggcggtggc accgcatgca gcgatgataa ttggaattac cttttatccg
      961 gtaagtatga tgaaacaatt agcggaaaat atgcatttga tgcagtaata atgcacacat
     1021 attattcagc tgataactgg gaccattttg tgtatgacat atataacttt gttctgagca
     1081 acttgacgcc gtgcaatcca agtccttacc caagtatttg ggatgacctt tggcattttg
     1141 gatatgcaga tcccgatgtg cggcttcaaa caatgtttga aaaacttagg gggaatggat
     1201 atgaagaagg tgaaaccgga aattacatgg attttttatc aaatcatgca actacaccgg
     1261 tagatttgta taaatattca atgctaagct cctttaaaga gttcggcaac caacttgacc
     1321 tggacagaac agatgccggc aagaaagatc tttgggtaac tgaatggaat aataagactg
     1381 tgataacgga gcacaccacc gaccattggc cgctagattg gccggtaggc attgacatta
     1441 cgaatgatct gcaacaaaaa atgctggaag cgccttctaa tacattttta agtgggtatt
     1501 tacttatgcg ttggtggttg aaggaaatag aaatgaatta ttatatcaac gattttagac
     1561 aaaatttttt caccatttca accatgcagg gttatggggg cggtgtttgg agcgatttat
     1621 tgacgctcac taccggagat gaaagggaag cagagggaac tgatattgtt ccctgggaag
     1681 atataggcgg cactattcct gaaacaccag gttgtgacta cattggagaa tcaggtaaaa
     1741 aattccttga aagaaattac tggatgcgca gaatgaatta ttatgttgcc agactattta
     1801 gtgacatttc gagaaaccat ttgctatttg tgccatcggc cttcctgtat gacgaacaca
     1861 atattaatct tccgccgatg gtgtttattg atgatgatgt aaatcctcaa tatatttata
     1921 tttatttcac caatgtaaaa gatgtgatac agaattatac tttggatccg caatttatac
     1981 gccttatgtt tactcccgat gcagatgatg taattttggg tattcctcaa ataaattata
     2041 tcattgccaa tgaattgtat gcaacatttg gtaatactaa attgtataat accagtttaa
     2101 acaattgcta tgatgattac gaacagccta catacctagc taacgcacaa atggtttggg
     2161 gtgattctta tcctccatca tgtataggag ctattcaccc ggatcattgc tttacagcac
     2221 ttccttatac ta
//
LOCUS       DAOQOU010000288         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_42834_length_2220_cov_5.292841, whole genome shotgun sequence.
ACCESSION   DAOQOU010000288 DAOQOU010000000
VERSION     DAOQOU010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42834_length_2220_cov_5.292841"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1225)
                     /locus_tag="PLM90_14525"
     CDS             complement(185..1225)
                     /locus_tag="PLM90_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMZ90649.1"
                     /translation="MKIKLLMFVTALAFGLSSRAQAPVESYNIKVSQKLELRGLTHIR
                     YQLFDDSTKIDAFDLRRARLDFRGDVAPKMAYRLHVEMANSPKILDATFVYKPYEWLN
                     LNVGQSKTPYCYDNLYSPWTLLTVSRTQIDNALSNRESDLYGNQNGRDLGFWLSGKYS
                     IGEEGNKRPIIDYTLGVYNGSGINVADANQDKDYGAALGVSPIKDLWLFGRYYTGTGR
                     TTLEPETDATKTRFGGNITYKYKDFLFEAEYLSGTDESDSLAQLDRNGYYITLGYTPI
                     KDKLQVIARLDNYDKDANKDLNIVNKYILGASWFFTKNTRVQLEYDIVAEEDADNQIK
                     NNLFAIQFQAAF"
     gene            complement(1332..1772)
                     /locus_tag="PLM90_14530"
     CDS             complement(1332..1772)
                     /locus_tag="PLM90_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ90650.1"
                     /translation="MLSKKAKYGLKAMFYLAKQFGKGPVLISDLAEEEHIPKKFLELI
                     LLELKKNGLLHSKMGKGGGYYLNKPPGEVTVGQVVRILDGPLAPIPCVSKTAYRKCDE
                     CENEKTCEIRKIMMQVREASATILDHTSLLEAPKALFTVARKNK"
     gene            complement(1791..>2220)
                     /locus_tag="PLM90_14535"
     CDS             complement(1791..>2220)
                     /locus_tag="PLM90_14535"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90651.1"
                     /translation="HKKGLVLIQAIKVDHPVLQYVITNDFHGFYEKEIAERRQFEYPP
                     FTRLIRITFRHSQKERVFATSDWFAAGVRASLGDRLLGPAVPGIPRIRNKYLMELMVK
                     FPNNRQAGDMVKEIIKNQISVMMSDPVHKKVDFLLDVDPM"
BASE COUNT          622 a          514 c          361 g          723 t
ORIGIN      
        1 atgctaccgg tgcatttaca aaggctccat tcaccattaa gatcattccg agtgctacac
       61 ttataattgc ttttttcatt tgaatttttt tatgtactat ttattctatc gtttttgtag
      121 tactttgtat ccataaaaag tgctgatcaa attcagcact ttttatggac acatcattag
      181 ctatttagaa tgccgcttga aactgaatag caaacaaatt attttttatt tgattatcag
      241 catcctcttc ggcaactatg tcatattcaa gctgaacccg agtgtttttt gtaaaaaacc
      301 aactcgctcc aagtatatat ttatttacaa tattcagatc tttgttagca tccttatcat
      361 aattgtcaag acgcgcgatt acctgcaatt tgtccttaat tggtgtatat cccaacgtaa
      421 tgtaatatcc attgcgatct aattgcgcaa ggctatcact ttcatccgtt ccactcaaat
      481 actcagcctc aaacaaaaaa tctttgtatt tgtaggttat attgccacca aatctggttt
      541 tagtcgcatc tgtctccggc tctagtgttg tcctgccggt tccggtataa tatctaccaa
      601 acaaccacag atctttaata ggagaaacac ctaatgcagc accgtaatct ttatcctgat
      661 tcgcatccgc aacatttatt ccggaaccat tgtaaacgcc taatgtatag tcgataatcg
      721 gtctcttatt tccctcttct ccaatgctgt acttaccgct taaccaaaaa ccaagatcac
      781 gaccgttttg attgccatac aagtcagact ctcgattcga caatgcgttg tcaatttgcg
      841 tgcgacttac agttaataaa gtccatgggc tgtataagtt atcataacaa tatggtgtct
      901 tactttggcc gacattcaaa ttcaaccatt catatggttt gtacacaaat gtggcatcaa
      961 gtattttcgg tgagttagcc atttcgacat gcaaacgata ggccattttt ggcgctacat
     1021 caccacgaaa atccaatcgt gcgcgacgta aatcaaatgc atcgattttt gtcgaatcat
     1081 caaataattg atagcgaata tgagtcaatc ctcgcaattc aagtttctga gataccttga
     1141 tgttatacga ttcaaccggt gcctgcgctc tagaactcaa tccaaaagcc agcgctgtta
     1201 caaacattag tagtttaatt ttcatagatg tgttatttgt cgagggcaaa tatctatata
     1261 atctatcatt ttagtagttt ttataaacat atttattttt ataaattgta atcgcccgat
     1321 ctgcaatatg tttatttgtt ttttcgcgca acggtgaata gcgcttttgg tgcttctaaa
     1381 agactggtat gatccaatat tgtggcagat gcctcacgaa cctgcatcat aatcttccgg
     1441 atctcacagg ttttttcatt ctcacactca tcacatttgc gatacgccgt tttacttaca
     1501 catggaattg gcgccaatgg tccatctaat atgcgcacta cctgccctac agttacttca
     1561 cccggaggct tgttcagata gtatccgccc cctttaccca ttttactgtg caacaaccca
     1621 tttttcttca actccaacaa tatgagctca aggaattttt taggaatatg ctcttcttcg
     1681 gcaagatcag aaatcagtac cgggcccttg ccaaactgct ttgcaagata aaacatggcc
     1741 ttgagcccat acttcgcttt tttcgataac ataactaggg taaaaatacg tcacataggg
     1801 tcaacatcca gcagaaaatc taccttctta tgcacagggt cggacatcat gacggatatc
     1861 tgatttttga tgatttcctt gaccatatca cccgcctgcc tgttgtttgg gaacttcacc
     1921 atgagctcca tcaaatactt attcctgatg cgaggaatac ccggcaccgc aggtcctagc
     1981 aatctatcac ccaatgaggc tcttactccg gccgcaaacc aatcgctcgt tgcaaatacc
     2041 cgctcctttt gactatgtct aaaggtaatc cttataagcc tggtaaacgg cggatactcg
     2101 aattgtcttc gctcagcgat ttccttctca taaaatccat ggaaatcatt ggttatcaca
     2161 tattgcaata ccggatgatc aacttttatc gcctgaatca atactaaacc ctttttatgc
//
LOCUS       DAOQOU010000289         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_43751_length_2196_cov_4.543204, whole genome shotgun sequence.
ACCESSION   DAOQOU010000289 DAOQOU010000000
VERSION     DAOQOU010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43751_length_2196_cov_4.543204"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLM90_14540"
     CDS             <1..372
                     /locus_tag="PLM90_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90652.1"
                     /translation="VTFGSEYRSRCHLYISIDSLNEKDLIVQPLSQEEVPIFFNEHPE
                     YIWVAEGSYDEAQFISLLTTNERAISIIEFEIENKLKPETTGTTLIEACKSDLIKDYR
                     LSIEYRSKYRQAVEGLGLAGN"
     gene            436..1014
                     /locus_tag="PLM90_14545"
     CDS             436..1014
                     /locus_tag="PLM90_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90653.1"
                     /translation="MKKILLALTMMLSAFTYAQSDDTEHHPFRIELEPSGFIYRGYGV
                     QALYDLNYIGTMTGGLYFSSLDVPDWTHKGMFDEVSDTADVRLGFEAALVYRYRFLIG
                     NGASAPYVGAIAGYEYFDITQAPYPKTVRLSTFVVTPYIGYEVYVWNQRLYVNPQVRS
                     VFYINQQSSDNDRPETLSNLFILPTISLGYTL"
     gene            1133..2050
                     /locus_tag="PLM90_14550"
     CDS             1133..2050
                     /locus_tag="PLM90_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ90654.1"
                     /translation="MITLTVIQPVITACSEDFNKMHKHQSGAFCDSCNKVVHDLSALN
                     TEQLITFIQQHPGACGRVQSNAMRMSVTVKDDIKPSKSFSMRFAFALLVVFGPLLFSC
                     TEEQHHQIKEIAQQEFFENEAINSMDTATEEIAIQNPEYTAVKQTILDEQQTFISEIT
                     DTTEIELETVNVSVPSNSKTQCFFAGGLSYTKIVNVISIETDTTDLQPIKESESLLTN
                     RTLSLGVYPNPTVSQAEVKYSLKQSGPLLVTIFNLAGQEMLPLVNDQNVDAGQYTHNI
                     DVASWPSGVYLVVLVSGTEQKNFKFTVAH"
BASE COUNT          669 a          420 c          448 g          659 t
ORIGIN      
        1 gttacatttg gaagtgaata tcgcagcaga tgtcacctgt atatctccat tgacagtctc
       61 aacgaaaagg atttaattgt tcagcctctt tctcaagagg aggttccgat attttttaat
      121 gagcatcctg aatatatttg ggttgctgaa ggatcttatg atgaagctca gtttatttca
      181 ctgctcacga cgaatgaacg cgcgatcagc attattgaat ttgaaattga aaacaaactc
      241 aaacctgaaa ctacaggcac cacattgatt gaggcatgta aatctgacct cattaaggat
      301 tatcgcctct ctattgaata tagaagtaaa tatcgccagg cggtagaagg attggggctt
      361 gcaggcaatt gacatttacc cttccatttg cagcatttgt ttgttaactt tgggtatcat
      421 caaacccaat atgccatgaa gaagatactg ttagctctga ccatgatgct aagtgctttt
      481 acatatgctc aaagcgacga tacggagcat catccttttc gcatagaatt ggagccttcc
      541 ggatttattt atcgcggtta cggcgttcag gcattgtatg atttgaatta tatcggcacc
      601 atgacaggag gtctgtattt ttcatcttta gatgtacccg attggacaca caagggtatg
      661 ttcgatgaag tgagtgatac tgcagatgta cgtttgggat ttgaagcggc cctcgtttat
      721 cgatatcgct tcctgattgg aaatggtgct agcgctccat atgttggtgc aattgcgggg
      781 tatgaatatt tcgatatcac acaagcgccc tatccgaaaa cagtacgatt aagcactttt
      841 gttgttacac cctatattgg ttatgaggtt tatgtttgga accagcgatt atatgtaaat
      901 ccgcaggtgc gcagtgtatt ttatatcaac caacaatcat cggataacga cagaccggag
      961 accctctcca acttgtttat actcccgact atttcgctcg gttacacact ttaaacacgg
     1021 ttgtatacgt ttactaattt gtaagattag tgtcaataaa agcagcttga cattttagtg
     1081 acttattggg gcggcaaaga tgctatcttg ctgaattata aacttctcca ctatgatcac
     1141 ccttaccgtt atccaacccg tcattactgc atgtagtgaa gatttcaaca agatgcataa
     1201 acaccaatcc ggtgcatttt gcgattcctg taataaggta gtgcatgacc tctccgcatt
     1261 gaatacggaa cagttgatta catttattca acaacatccg ggggcgtgcg gaagagtaca
     1321 gtctaatgcg atgcgcatgt cagttacagt aaaagatgat attaagccgt caaaaagttt
     1381 ttcaatgcga tttgcatttg cactgttggt agtttttggt ccgctattat tttcatgcac
     1441 cgaagagcaa catcatcaaa taaaggaaat tgctcagcag gaattttttg agaatgaggc
     1501 aataaatagc atggataccg caaccgaaga aattgccata caaaaccctg aatatactgc
     1561 tgtgaagcaa acaatattgg atgaacaaca gacattcatt tcagaaatta cggatactac
     1621 cgagattgaa cttgaaacgg taaatgtaag tgttccatca aattcgaaga ctcaatgttt
     1681 ttttgcaggt ggattaagct acactaagat agtcaacgtt atttcaatag aaaccgatac
     1741 aacagatttg cagcccataa aagaatcgga aagtctattg accaatagaa cattatcact
     1801 tggtgtatat ccaaacccta cagttagcca ggcagaagtc aaatatagtt taaaacaatc
     1861 cggaccgctg ttggtgacaa ttttcaattt agcaggacaa gagatgttgc cactcgtaaa
     1921 tgaccaaaat gttgatgccg gacagtatac ccataacatt gatgttgctt catggccttc
     1981 gggtgtttat ttggtagtgt tggtttcagg aactgagcag aagaatttta agtttacggt
     2041 ggcgcattaa ttattgcaat aaaccattga cattaatgtt gttaattgca ttgtgttttt
     2101 tttaaccacg gagggcacat aggtgagcac agctttacac ggagaaaatt ataaaattaa
     2161 ctttaatcct aaacccaaga ataaatcatc cgctcc
//
LOCUS       DAOQOU010000290         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_45292_length_2155_cov_5.851429, whole genome shotgun sequence.
ACCESSION   DAOQOU010000290 DAOQOU010000000
VERSION     DAOQOU010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45292_length_2155_cov_5.851429"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PLM90_14555"
     CDS             <1..1019
                     /locus_tag="PLM90_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90655.1"
                     /translation="STCFYLLSYLQEYTDYHPDQYGRFPFSNSIQHHFDCIDKNLVAQ
                     YFTLLYEQTPKLKQLVSKKEHASVVFLSHDIDSAYGALAENGKWLLKHGKVGALMQLI
                     FNHYVRTPDHLLLDKIMQIEDAYDVKSVFFWLARSGRGTRLINNADYNLSDSKVRSIQ
                     QHISEKGWVNGLHKSAGKSTMTADYNSVREFAEPVNRNHYLLTELPGTFNEIEAAGIQ
                     MDCTMGFAEAPGFRNSYGLPVRPYHFKEKREYNFIEVPLHIMDTTYRYYRKESGKEAE
                     RLIIDFLEHNPQDAVISILWHNNYFFDRTDTSWLDAYKAVLQYMHEKQIKSVLPKELL
                     EEYL"
     gene            complement(1025..1834)
                     /locus_tag="PLM90_14560"
     CDS             complement(1025..1834)
                     /locus_tag="PLM90_14560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ90656.1"
                     /translation="MQYDPVKRSLGKVFNTTPVLRIVFYKMLDLLLLRAWHIHKALRQ
                     WSKGKKETEQHILDAGSGFGQYSYFLSGMSNKWKFLSVDVKEEQIGDCNNFFQKIGRK
                     NIRFQVKDLTTFTIPDHFDLVVCVDVMEHVLEDVQVFKNYHTSLKTGGMVLISTPSDQ
                     GGSDVHEETGSSFIEEHVRDGYNIQEIQDKLTSAGFTRTEAHYAYGTPGKISWRLSMK
                     YPLQLLNISKLFFIILPVYYIITFPFCLLLNACDVAMSHKTGTGLIVKAWK"
     gene            complement(1836..>2155)
                     /locus_tag="PLM90_14565"
     CDS             complement(1836..>2155)
                     /locus_tag="PLM90_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90657.1"
                     /translation="ISYTSKSSVEMPAVFSEIWYRGNQDWKAYIDGKYVDHFRANYLL
                     RGLWVPAGEHEIVFKFEPASFAAGKKVSTASSGLILLLVAIGFFLAGRKAIQENKQTE
                     EQA"
BASE COUNT          648 a          418 c          430 g          659 t
ORIGIN      
        1 taagtacttg tttttacttg ctatcctact tacaggaata caccgattat catccggatc
       61 aatatggtcg ttttccgttt tccaatagta ttcagcatca ttttgattgt atcgataaaa
      121 acctggtagc gcaatatttc acattattat acgagcagac tccaaaattg aagcaattgg
      181 ttagcaagaa agagcatgca tctgtggttt ttctgtcgca tgacattgat agtgcttacg
      241 gcgctttggc agaaaatggt aaatggcttt taaaacatgg aaaggtaggc gcattgatgc
      301 aattgatttt taatcattat gtgcgcacgc ctgatcatct gcttttggat aaaatcatgc
      361 aaattgaaga tgcatacgat gtgaaatcgg tgtttttttg gttggcgcgt tcaggtcgcg
      421 gaacacgttt gatcaacaat gcggattata atttgtctga tagtaaagtt cggtccattc
      481 aacagcacat atcggaaaag ggatgggtaa acgggttaca taaaagtgcc gggaaatcga
      541 caatgactgc tgattacaat tcagtaaggg aatttgcaga gccagtaaat cgcaatcatt
      601 atttattgac tgaacttccg ggaacattca atgaaattga ggctgcagga attcagatgg
      661 attgcaccat ggggtttgca gaagcacctg gatttagaaa tagttacgga ttgccggtca
      721 ggccatatca ttttaaagaa aagcgcgaat ataattttat tgaggtgcca ctccatatta
      781 tggatactac ttaccgatac tatcgaaaag aaagtggtaa ggaagctgag cgtttgataa
      841 tcgacttttt agagcataac ccgcaagatg cagtaatctc tattctgtgg cacaataatt
      901 attttttcga cagaacggat acctcatggc ttgatgcata caaggctgtg ctgcaatata
      961 tgcatgaaaa acaaatcaaa tctgtacttc cgaaggagtt gctcgaagaa tatttgtaag
     1021 tagtttattt ccaggccttt acaattaatc ctgttcccgt tttatgcgac atagccacgt
     1081 cgcaggcatt cagtaataaa cagaatggaa aagtaataat gtaataaaca ggcagaataa
     1141 taaaaaacag tttcgaaata tttaataatt gcaaaggata cttcattgaa agtcgccatg
     1201 aaatttttcc gggagttccg taagcgtagt gagcctcagt tcgagtgaag ccggcagatg
     1261 ttagtttatc ttgtatttcc tgaatgttat acccatcgcg aacatgttct tcgataaatg
     1321 acgagccggt ttcttcatgc acatctgagc cgccctgatc gctaggagta gaaatcagca
     1381 ccatgccacc tgtttttaat gaagtatgat aatttttaaa cacctgcaca tcttctaaca
     1441 cgtgttccat tacatctaca catactacca ggtcgaaatg atcgggaatg gtaaaagttg
     1501 tcagatcttt tacctgaaaa cgtatatttt ttctgccaat tttttgaaag aaattattgc
     1561 aatcgccaat ttgttcttct ttgacatcga ctgacaggaa tttccatttg ttgctcatgc
     1621 cgctgaggaa atagctgtat tgaccaaacc ctgagccggc atcaagaata tgttgttctg
     1681 tttccttttt tcctttcgac cattgacgca aagctttatg gatatgccat gctcttaaca
     1741 aaagcaggtc gagcatttta taaaatacta tgcgcaaaac cggggtagta ttaaatactt
     1801 ttcccagact gcgtttaacg ggatcgtact gcatgtcagg cttgctcctc tgtttgtttg
     1861 ttttcttgaa tcgcttttct tccggcgagg aaaaaaccta tcgccactaa taataatatc
     1921 aaccctgaag atgctgttga aactttttta cctgcggcaa atgaagccgg ctcaaatttg
     1981 aaaacaatct catgttcacc tgcaggaacc cacaatccac gcaacaaata atttgcgcgg
     2041 aaatgatcaa catattttcc atcgatatag gctttccaat cctgattgcc gcgataccat
     2101 atttccgaga aaaccgcagg catttctaca ctacttttcg atgtatagct gatct
//
LOCUS       DAOQOU010000291         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_46946_length_2115_cov_7.443204, whole genome shotgun sequence.
ACCESSION   DAOQOU010000291 DAOQOU010000000
VERSION     DAOQOU010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46946_length_2115_cov_7.443204"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLM90_14570"
     CDS             complement(<1..741)
                     /locus_tag="PLM90_14570"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HMZ90658.1"
                     /translation="MKKILVVVGTRPNFIKVALFKSMAAMHGFDLRIVHTGQHYDAAM
                     ADVFFRQLALQPDYFLELPSLQPEQQVILIQAKLNRLMGSTFMPDLVMVVGDVNSTRA
                     AAEAAHILNIPIAHVESGLRSFDQSMPEEINRIVADNLASLFFITEQSGIDNLREEGK
                     PENKMHLVGNTMIDTLVHFAPLIDQQNILEQWQLEEKRYYLVTLHRPSNVDEKNGLQQ
                     AIECLNWLSEDLPVVFPIHPRTKKQLLQF"
     gene            844..1782
                     /locus_tag="PLM90_14575"
     CDS             844..1782
                     /locus_tag="PLM90_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ90659.1"
                     /translation="MPGETILIIGACGQIGAELTMFLRHQYGGSNIIAADIKAPGGEL
                     LESGPFEEINVLDQAGIAGIIDKYKVTQIYNMAAMLSATGERFPHKAWELNMEGLINT
                     LELAREKNIRKMYWPSSIAAFGPTTPKVDTPQITIMEPNTIYGISKQAGERWCEWYHQ
                     KFGLDVRSLRYPGIISYKTQPGGGTTDYAVDIYFKAKAQGAYTCFLAHDMMLPMMYMP
                     DAIRATVELMEASADKISVRSSYNLAAMSFNPAMVGESIRKYMPDFKLDYEIDFRQKI
                     AETWPGSIDDHQARQDWGWKHAYDLDAMTKDMLNHV"
     gene            1873..>2115
                     /locus_tag="PLM90_14580"
     CDS             1873..>2115
                     /locus_tag="PLM90_14580"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMZ90660.1"
                     /translation="MKKIAVMTSGGDSPGMNACIRAVVRTGLYHGLEVYGIRRGYEGM
                     IDGDFFKMESYHVSNIIQRGGTILKTARSERFMTAEG"
BASE COUNT          651 a          418 c          475 g          571 t
ORIGIN      
        1 aaattgcagt aattgttttt tcgtacgcgg atgaattggg aaaacaacag gtagatcttc
       61 agaaagccaa tttaaacatt ctatagcctg ttgtaatcca tttttttcat caacattaga
      121 tggccgatgt aaggttacga ggtaatatct tttttcttcc aattgccatt gctccagaat
      181 attttgctga tcgatcagtg gcgcaaaatg aaccagggtg tcgatcatgg tattcccaac
      241 caaatgcatt ttattttcag gtttaccttc ttcgcgaagg ttatcgatac cactttgttc
      301 agtaataaaa aacagtgatg cgaggttatc tgcaacaatg cgattgatct cttcaggcat
      361 ggactgatcg aagctgcgca aaccactttc cacatgggcg ataggaatat ttaaaatgtg
      421 tgctgcttca gctgctgccc tggtagaatt gacatcgcca acaaccatta ccagatctgg
      481 cataaaggtg cttcccatca ggcgattcaa cttggcttga atcaaaatca cttgttgctc
      541 aggctgtaag gaaggaagtt cgaggaaata atccggttgc aatgccaact gtcgaaagaa
      601 gacatcagcc atggcagcat cataatgttg accggtatgt acgatcctga gatcaaaacc
      661 atgcatggca gccatggatt taaacagggc tactttaata aaattcgggc gtgtgcccac
      721 taccaccaat atctttttca tcaagattct acgaccccgt aaaggtaagc gtttataact
      781 tcctgctttc ggaatttgca attcttgtcc atagggctat ttttgtttcc ctaacaacaa
      841 gatatgccgg gagaaacgat actgataatt ggagcctgtg gccaaattgg cgctgagttg
      901 acaatgtttc tgcgccatca gtatggtggt tcaaatatta tagcagcgga tattaaagca
      961 cctggaggtg aattgttaga gtcgggacca tttgaagaga tcaatgtatt ggatcaagcc
     1021 ggcatcgcag gtattattga taaatataaa gttacccaga tctacaatat ggcagccatg
     1081 ctttctgcca cgggcgagcg ctttccacat aaagcttggg aattgaacat ggaagggctg
     1141 attaatacac ttgagcttgc cagagagaaa aatattcgca agatgtattg gccaagttct
     1201 atcgctgcct tcgggccaac aacaccgaaa gtggatacac cgcagatcac catcatggag
     1261 ccaaatacca tttacggtat cagcaagcag gcaggagaaa gatggtgcga atggtatcat
     1321 cagaaatttg gtctcgacgt aagaagtttg cgataccccg gaattatcag ttacaaaacg
     1381 caacccggcg gaggaacaac agattatgcg gtcgacattt attttaaagc aaaagcacaa
     1441 ggagcctata cctgttttct ggcacatgat atgatgttac caatgatgta tatgcctgat
     1501 gccatcagag caacagttga attaatggag gcatctgcag acaaaatttc tgttcgttcg
     1561 tcatataatc tcgccgctat gagcttcaat cctgccatgg taggggagtc tatcaggaaa
     1621 tatatgcctg atttcaaatt agattacgaa atcgatttca ggcaaaaaat agctgaaaca
     1681 tggccgggaa gtatcgatga tcatcaggct cgtcaggact ggggctggaa gcacgcgtac
     1741 gaccttgatg cgatgacaaa agatatgctc aatcatgtat aatacttact ttgtggaaac
     1801 ccgcaagata aaaaagatgt tagtttaata ttgacatcct atttttgtac ctgatcaggc
     1861 aataaactaa tcatgaaaaa aattgcagta atgacatccg gtggcgactc accgggaatg
     1921 aatgcatgta ttcgcgctgt agtgcgcaca ggtttatatc atggacttga agtatatgga
     1981 attcgcagag gatatgaagg gatgatagat ggagatttct ttaaaatgga atcctatcat
     2041 gtcagcaata tcatacaacg tggaggaacc atcctaaaaa cggcgcgaag cgaaagattt
     2101 atgactgcag aagga
//
LOCUS       DAOQOU010000292         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_48059_length_2088_cov_5.263158, whole genome shotgun sequence.
ACCESSION   DAOQOU010000292 DAOQOU010000000
VERSION     DAOQOU010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48059_length_2088_cov_5.263158"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1383
                     /locus_tag="PLM90_14585"
     CDS             115..1383
                     /locus_tag="PLM90_14585"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMZ90661.1"
                     /translation="MHVDVLLGLQWGDEGKGKVVDYLAEQYNVVARFQGGPNAGHTLI
                     LNNGEKFVLHTIPSGIFLNNCLNVVGNGVVIDPITFRMEVQKLEKAGVDVTHRLVIAR
                     KAHLILPTHKMLDAASEASKGQDKIGSTLKGIGPAYMDKTGRNGLRVGDIESPDFLKK
                     YNTLRDKHFQLLRIYGEHHNEVPNEQEWMDAIAFLKQLPFVDAEYLVDEAMHQGKTIL
                     AEGAQGSMLDVDHGTYPFVTSSNTITAGVCTGLGVAPQRVKEVIGISKAYCTRVGGGP
                     FPTELFDADGEELRKIGNEFGATTGRPRRCGWIDIPALKYTIMLNGVTQLAITKVDVL
                     NTFTKIKACVAYKINGVITDRVPYDIVDAVIEPVYEEFPGWNCSLDGCKSKADLPEAL
                     QNYLQFLEWHLKTRITMLSAGPERDKLILL"
     gene            1435..>2088
                     /locus_tag="PLM90_14590"
     CDS             1435..>2088
                     /locus_tag="PLM90_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90662.1"
                     /translation="MRTSAQFQVGEDFRSRLSEFAGRYEEYALLDSCGNTEYGANTFD
                     YLLACAAVAVIVHTEGESVSAELKAAIHEHGDYLFGYLGYELNALQEPVFSSPQQLFQ
                     LPAMRFFVPGILVMVKDGTATISIHESTCDTAENVFRTISEISEENLGNFLHNPSPIT
                     LKPLVSASDYLSTVDKIRQHIIDGDIYEMNYCQPFVANDVNINPTNLFNRLCSIAKAP
                     "
BASE COUNT          572 a          465 c          491 g          560 t
ORIGIN      
        1 ccactcacca ctcaccactc accactcacc actcaccact caccactcac cactcgccac
       61 ttaccacttg tcaatgcccc taagcttccc taacttcgca ctttaatacg cgatatgcac
      121 gtagatgttt tactaggcct gcagtggggc gatgaaggaa agggaaaagt tgtcgactat
      181 ctggcagagc aatacaatgt ggtagcccgt ttccagggcg gtccgaatgc aggacataca
      241 ctgatcctta acaatggcga gaagttcgtg ctccatacca ttccgtcagg gatatttttg
      301 aataattgtc tgaatgtagt gggaaatgga gtggtaattg accctattac cttccgtatg
      361 gaggtacaaa aactggaaaa agcgggcgta gatgtcactc acaggctcgt aattgcacgc
      421 aaggcccatt tgatactacc tacgcataag atgcttgatg ccgctagcga ggctagcaaa
      481 gggcaggata agattggatc aacgctcaag ggtatcggtc cggcttacat ggacaaaacc
      541 ggtagaaacg gcttgagggt gggtgatatc gaatcgcctg atttcttgaa gaaatataat
      601 accctgagag acaagcactt ccagctgtta cgtatttatg gtgaacatca taatgaggtg
      661 ccaaatgagc aggaatggat ggatgcaata gcttttttga agcagttgcc gtttgttgat
      721 gccgagtatt tggtggatga ggctatgcat cagggaaaga ccatacttgc cgaaggtgca
      781 caaggttcta tgcttgatgt agaccacggc acttacccat ttgttacatc cagcaatacc
      841 ataacggcgg gcgtttgcac cggcttaggt gttgcccctc agcgcgtaaa agaggtgata
      901 ggtatctcta aagcttattg cacacgcgta ggcggagggc cttttccaac ggaattattt
      961 gatgccgacg gagaagaatt aaggaaaata gggaatgaat tcggcgctac taccggtcgc
     1021 ccgcgtcgct gtggatggat tgatattccg gcgctgaaat ataccatcat gctcaatggt
     1081 gtaacgcagc ttgctatcac aaaagtggat gtgctgaata ccttcaccaa gatcaaagca
     1141 tgtgtagcat acaagatcaa cggcgtgatc actgatcgcg taccttacga cattgttgat
     1201 gcggtgattg agcccgttta tgaagaattc ccaggttgga attgttcgct tgacggctgt
     1261 aaatcaaaag cagatttacc tgaggcgttg cagaattatt tgcagttttt ggaatggcat
     1321 ctgaaaacac gtatcaccat gctatctgct ggtccggaac gcgataagct cattctgtta
     1381 taagcagtaa ttattttttg ctgagcgatc ggcatcttac ttttcaaaac ggcattgcgt
     1441 acgagtgcac aatttcaggt tggggaagat ttcagatccc gactttcgga gttcgccggg
     1501 agatatgaag aatatgccct gctcgatagc tgcggaaata cggagtacgg tgctaatacc
     1561 ttcgactatc ttctggcttg tgctgcggtt gcagtgattg tacataccga aggcgaaagc
     1621 gtttcggcgg aactgaaggc tgctatacat gaacatggcg actatctttt cggctacctg
     1681 ggttacgagt taaatgccct gcaagagcct gttttttcct ccccccaaca attatttcaa
     1741 ctgccggcca tgcgcttttt tgtgcccggt attttggtca tggtgaaaga tggtactgca
     1801 actatttcta tccatgaaag cacctgtgat actgctgaaa atgtatttcg aaccatctcc
     1861 gaaatttcag aagaaaactt aggcaatttt ttacacaatc cttctccaat aacattaaag
     1921 ccgcttgtaa gcgcatctga ttacttgtca acggtcgata aaatcagaca acatatcatc
     1981 gacggcgata tttatgaaat gaattattgt cagccatttg ttgcaaacga tgtaaatatt
     2041 aatccaacca atttattcaa ccgcttatgt tcaatcgcca aagctcca
//
LOCUS       DAOQOU010000293         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_48793_length_2071_cov_6.898810, whole genome shotgun sequence.
ACCESSION   DAOQOU010000293 DAOQOU010000000
VERSION     DAOQOU010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48793_length_2071_cov_6.898810"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2071
                     /locus_tag="PLM90_14595"
     CDS             <1..>2071
                     /locus_tag="PLM90_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90663.1"
                     /translation="TIRIFSGAGGKFMHPLGSPVDPAEPEGMWNGGYGFSWEELVVFY
                     DKTDGDDDAPSTTSIISNMIDGVCNGCGAVGTGWMEAKFVGDFEDFYNKAAAQPTFDP
                     ADYGTGMELDRPLYINQCIDLIGQIESANPGHVVDVIYVVNIQTQTADDVLAVIDYLR
                     DHDVHVTGIEMGNEVYFDFHALSMGLDDFPDYWNYINGVNFGDLEDILPADVYADHDY
                     IGKLKGNADYYDLKIGLPAQNTPNCGAEYDFPVTPPGIATHELTPVITDPGGDPCPCF
                     YPDWNVKMTEYYDETVGTGAATRYAFDAIIFHTYYGPTNNTGTCSENSNWRDILLTNL
                     HTGFDPDDLEKDLDFELFPDPWNYVEDVAPFAPSAPDGNLIDAFYGITGIHHPLTDSP
                     LLPGNYKDFTRTRLDISYEEHAAQMHFTAADTDPKNKQVWVTEYNLDDKIDMPKVVIM
                     GVEYPAIQAENQEIVTPIVAAATNTFAHTIMMQHWFLWHLKASFDPDYRNNFLTVATT
                     QNFLSAGSTIGLMTRSDKADQERLHEIATCSDPEIPVFYVRKAQYFGSLLQKPIIAND
                     LKYLKSNISLGTLNNNTPPTLFIKKDGFESTDDTVYVVYDNVTDAYQTYIIEPGTLVN
                     DYGGAAGYEITLGSGTIEGDMLDADQLYSSAGSVAHFKFNEGYSNCAVSIDNENRFEI
                     DALESLSP"
BASE COUNT          623 a          400 c          454 g          594 t
ORIGIN      
        1 aaaccattcg catttttagc ggtgcaggag gaaaattcat gcatccgctg ggttctcccg
       61 tagaccctgc agagccggaa ggcatgtgga atggcgggta cggattcagt tgggaggaat
      121 tggttgtatt ttatgataag acagatggag atgatgatgc tccttctaca acatctatta
      181 tcagcaatat gattgatggc gtttgcaatg gttgtggcgc tgtagggact ggatggatgg
      241 aagctaaatt cgtgggtgac ttcgaagatt tttacaacaa agctgctgct cagccaacgt
      301 ttgacccggc agactatggc accggcatgg agttggatag gccattgtat atcaatcaat
      361 gtatcgatct gattggtcaa attgaatctg caaatcccgg acacgtggtt gatgttattt
      421 atgtagtcaa cattcaaacg cagaccgccg atgatgtatt agccgttatt gactacttac
      481 gagatcatga cgtacatgtt acgggcatag aaatgggcaa cgaagtttac tttgatttcc
      541 acgcactgag tatgggactg gacgatttcc ctgattattg gaattatatc aatggtgtga
      601 atttcggcga cttggaagat atccttccag cagatgtata tgcagatcat gactatatcg
      661 gtaagctaaa aggaaatgcg gattattatg atttgaaaat cggattaccg gctcagaata
      721 caccaaattg tggtgcggag tacgatttcc ctgtaactcc tccgggaatt gccacacatg
      781 aacttacccc ggttatcaca gatccgggtg gagacccttg tccatgcttt tatccggatt
      841 ggaatgtgaa gatgaccgag tattatgatg aaacagtggg tactggtgcg gctaccagat
      901 atgcctttga tgccatcatt tttcatacat attatggtcc aaccaataat accggaacat
      961 gcagtgaaaa ttcaaattgg agagatatcc tgctgacaaa tttacatacc ggctttgatc
     1021 ccgatgatct tgaaaaagat ttagacttcg aattatttcc cgatccatgg aactatgttg
     1081 aagatgtggc accattcgcc ccatcagcac ctgatggtaa cctcattgat gccttttatg
     1141 gtattaccgg cattcatcat ccacttacag attcaccgct cctacctggt aattacaaag
     1201 attttacaag aactaggctg gatatttcct atgaagagca tgcagctcaa atgcatttta
     1261 cggcagcgga taccgaccct aaaaacaaac aggtctgggt tacggaatat aatctcgacg
     1321 ataaaataga tatgccaaaa gtggtaatta tgggtgttga gtatcctgcc attcaagcag
     1381 aaaatcaaga aattgtaacg cctatagttg cagcagctac caatacattt gcacacacca
     1441 tcatgatgca acattggttc ttatggcacc tgaaggcaag ttttgaccct gattatagaa
     1501 ataatttttt aactgttgcc acgacacaaa atttcttaag tgcaggaagc accattggtt
     1561 taatgaccag atcagacaaa gcagatcagg agcgattaca tgaaattgca acctgcagtg
     1621 atcctgaaat cccggtattt tatgttcgaa aggcgcaata tttcgggtcc ttattgcaga
     1681 aacctatcat tgcaaatgat ctcaaatatc tgaaatccaa tatttcacta ggtacactga
     1741 ataacaacac ccctccgacc ttattcatta aaaaagatgg atttgaatca actgatgaca
     1801 cagtttatgt agtatatgat aatgtaacgg atgcttatca gacatatatt attgaaccgg
     1861 gaaccttggt aaatgattat ggcggagcag caggatatga aataacatta ggaagtggaa
     1921 ccattgaagg tgatatgtta gatgccgatc agctgtattc ttctgccggt tctgttgcac
     1981 attttaaatt caatgaggga tattcaaatt gtgctgtatc tattgacaat gaaaatcgat
     2041 ttgaaattga tgcattagaa tcactttcgc c
//
LOCUS       DAOQOU010000294         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_49444_length_2057_cov_5.782717, whole genome shotgun sequence.
ACCESSION   DAOQOU010000294 DAOQOU010000000
VERSION     DAOQOU010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49444_length_2057_cov_5.782717"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1684
                     /locus_tag="PLM90_14600"
     CDS             131..1684
                     /locus_tag="PLM90_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ90664.1"
                     /translation="MDTMIQENAMAAELKALGILSNNSGVSTGVNWLKGEGNGVESFS
                     PVDGSLISSCTSASREEYEQIMQTAAAAFKEWRMWPAPKRGEVVRQLGEKLREHKQDL
                     GKLVSYEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLSMHSERPLHRMYEQWHPLG
                     IVGVISAFNFPVAVWSWNAALAFVCGDVCIWKPSSKVPLSAIATQKIIAEVFKANNVP
                     EGVSCLMVSHSSIGEEMAKDARVPLISVTGSTRVGKRIGGIVGERLGRVIQELGGNNA
                     IIISEHANMNIAIRAAIFGAVGTAGQRCTTTRRLIIHESVYADVLNLLKKSYAQLRIG
                     NPLDQNNHVGPLIDRKAVEDYEKAIAQAMEEGGKMIVEGGVCTGEGYESGCYVKPCII
                     EASNNFKIVQHETFAPILYVMKYSTIEEALDMQNGVPQGLSSSIMSTNIYETEYFLSQ
                     QGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINYSTQLP
                     LAQGIKFDI"
     gene            1754..>2057
                     /locus_tag="PLM90_14605"
     CDS             1754..>2057
                     /locus_tag="PLM90_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90665.1"
                     /translation="MRTITCRIESPLVSEYTWVMKVFAKYIGFNVQFVDGNEDMLIAE
                     HGMGDIQVSHFFRMTYQSGDCHFKSYFRKELLHYTASNKPDYLSTCFYLLSYLQEYT"
BASE COUNT          583 a          362 c          512 g          600 t
ORIGIN      
        1 ctaaacgcat ttaatattag tttaatcgtt aaagcactgt tgaatgcacc taatgacgat
       61 actggctatg tcggttcaac ggtgttcatt cacctcaaaa tccgtaaatt tgcaccctaa
      121 atatacctag atggatacaa tgatacagga aaatgccatg gctgccgaat tgaaagcctt
      181 aggcattcta tcgaataata gcggcgtcag caccggcgta aattggttga aaggtgaggg
      241 aaatggcgtt gaaagcttca gtccggttga tggttcgctg atttcgtcgt gtacttcagc
      301 ctcacgcgaa gagtatgagc agatcatgca aactgctgct gctgcattca aagaatggcg
      361 catgtggccg gctccaaagc gcggagaagt tgtgcgtcag ctaggagaga aactccgtga
      421 gcataagcag gatctcggaa aactggtgag ctatgagatg ggtaaaagtc tccaggaagg
      481 ccttggcgag gtacaggaaa tgatagatat atgtgatttt gctgttggat taagccgtca
      541 gctgtatgga ttgtctatgc acagtgaacg ccctttgcat cgtatgtatg aacagtggca
      601 tccgctgggt attgttggcg ttatttctgc attcaacttt ccggttgccg tatggagctg
      661 gaatgctgca ttagcttttg tttgtggaga tgtttgcatt tggaagccaa gttctaaggt
      721 gccgttgagc gccatcgcca cacaaaaaat tatagctgaa gtatttaagg ccaataatgt
      781 tcctgaaggc gtttcttgcc tcatggtatc gcatagttca attggcgaag aaatggctaa
      841 agacgcacgt gttccattga tttctgttac aggatctaca cgtgttggaa aacgcatcgg
      901 tggtattgtt ggcgaacgtc ttggtcgtgt gattcaggaa ttggggggta acaatgccat
      961 tatcatatct gaacatgcaa atatgaatat tgccattcgt gcagcaatct ttggtgctgt
     1021 tggaactgca ggtcaacgat gtactaccac tcgtcgactt atcattcacg aaagtgttta
     1081 tgcagatgtg ttgaatctgt tgaagaaatc atatgcgcaa ttgcgaatag gaaatccatt
     1141 ggatcaaaat aatcatgtag gaccgctgat tgatagaaaa gctgttgaag attatgaaaa
     1201 agcaattgct caagctatgg aagagggcgg gaaaatgatt gttgaaggcg gcgtttgtac
     1261 cggtgaaggg tatgaaagcg gatgttatgt gaagccatgt atcatcgaag cttcaaacaa
     1321 tttcaaaatt gttcagcacg aaacatttgc tccgatctta tatgtgatga aatattcaac
     1381 tatcgaggaa gcgcttgaca tgcaaaatgg tgttcctcaa gggctttcat cttctatcat
     1441 gtcgaccaat atttatgaga ctgaatattt cctttcgcag caggggtctg attgcggtat
     1501 tgcgaatgtg aatataggca caagcggtgc tgagatcggt ggagcctttg gtggtgaaaa
     1561 agaaaccgga ggcggtcgcg aaagtggtag tgatgcgtgg aaagcatata tgcgtcgtca
     1621 aaccaatact atcaattatt ctacgcagtt gccattggct cagggaataa aatttgatat
     1681 ctgataaaat caactaattt agaccgtcat cttaggtttg aatatctttg atgacggttt
     1741 tttgtttatg caaatgcgaa ccattacatg cagaatagag tcgcctctgg tgtcggaata
     1801 cacttgggtg atgaaggtgt ttgctaagta tatcggattt aatgtgcaat ttgttgatgg
     1861 gaatgaagat atgctaattg ccgaacatgg catgggtgac atacaggttt ctcatttttt
     1921 cagaatgacg tatcaaagtg gtgattgtca ttttaaatct tactttcgca aggaattact
     1981 gcactacacg gcatcaaaca aaccggatta tttaagcacg tgtttttatc tattatcata
     2041 tttacaggaa tacaccg
//
LOCUS       DAOQOU010000295         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_52278_length_1996_cov_5.802164, whole genome shotgun sequence.
ACCESSION   DAOQOU010000295 DAOQOU010000000
VERSION     DAOQOU010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52278_length_1996_cov_5.802164"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLM90_14610"
     CDS             <1..549
                     /locus_tag="PLM90_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HMZ90666.1"
                     /translation="HFERAIEVPSDTFREQLPMAADMLKDKKDANIIMYCTGGIRCEK
                     ASAYMKHVGFTNVFHIEGGIIHYANTVKEQQLENKFIGKNFVFDDRLGERISPEIIAK
                     CHQCGTPADTHTNCVNPACHLLFIQCEACAAKYERCCSVACQDTIHLPEEEQKRLRSG
                     KMKDQNIFNKSRSRMMDLERPQ"
     gene            630..1883
                     /gene="metK"
                     /locus_tag="PLM90_14615"
     CDS             630..1883
                     /gene="metK"
                     /locus_tag="PLM90_14615"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMZ90667.1"
                     /translation="MPYLFTSESVSEGHPDKIADQISDALIDHFLAFDPNSKVACETL
                     VTTGQVVLAGEVKSKTYLDVQDIAREVIRNIGYTKSEYMFEANSCGILSAIHEQSGDI
                     NQGVERKKKIEQGAGDQGMMFGYACRETDNYMPLSLEIANLLLKELAAIRREGKVMTY
                     LRPDAKSQVTIEYNDDNTPMRIDAIVVSTQHDDFDEEKKMLASIKTDIQNILIPRTIK
                     KLPKRLHKLFDGKYKLFVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYAVRHIAKNMVAAGICDEVLVQVAYAIGVAEPVGLYVNTYGTAKVKM
                     QDGEIAKRIRKIFDLRPYAIEQRFQLRTPIYLETAAYGHMGREPKTITKRFVDGGGRK
                     KSIRVTLFPWEKLDYVDAIRKAFKL"
BASE COUNT          621 a          394 c          475 g          506 t
ORIGIN      
        1 catttcgaac gtgcgataga ggttcccagc gataccttca gagagcaact gccaatggct
       61 gccgacatgt taaaggataa aaaggatgcc aatatcatca tgtattgcac aggtggtatt
      121 cgttgtgaaa aggcaagtgc atacatgaaa catgtaggat ttacgaatgt gtttcatatt
      181 gagggaggca ttattcacta tgccaatacg gtaaaagagc aacaacttga aaataaattc
      241 ataggcaaga actttgtgtt cgacgacagg cttggcgagc gcatttcacc ggagatcatt
      301 gccaaatgcc atcaatgcgg cacacctgcc gatactcaca ccaactgtgt gaatccggca
      361 tgccacctcc tattcattca atgtgaggct tgtgctgcca aatatgagcg ctgttgttct
      421 gtagcctgcc aggataccat acatctaccc gaagaggagc aaaaacgcct ccgcagcggc
      481 aagatgaaag atcaaaatat ctttaataag tcgagaagtc gcatgatgga ccttgagcga
      541 ccgcaataag gctgcaattt ccacattcag ggttagtaca tataggggca agaagttatc
      601 tttgcaccta caaaaaacta taagcttaaa tgccttattt atttacgtcc gaaagtgtga
      661 gcgaaggcca cccagataag atcgctgatc agatatcaga tgcgttgatc gatcattttt
      721 tagcctttga cccgaacagc aaggttgctt gtgaaacact ggttactaca gggcaggttg
      781 tacttgccgg tgaagtaaaa tcaaaaacat atctcgatgt gcaggatatt gcgcgcgaag
      841 taattcgcaa tatcggttac acaaaaagtg aatatatgtt tgaagctaat tcatgtggta
      901 tcttaagtgc catccatgaa cagagcggcg atatcaatca aggtgttgaa cgaaaaaaga
      961 aaattgaaca aggcgccggt gatcagggga tgatgtttgg atatgcttgt cgcgaaaccg
     1021 ataattacat gccattatca cttgaaattg caaatttatt attgaaagaa ctcgctgcca
     1081 ttcgtcgcga aggaaaagtg atgacatacc ttcgtccgga tgcaaaaagt caggtaacga
     1141 ttgaatacaa tgacgacaat acgccaatgc gcatagatgc aattgtagta tctacacagc
     1201 atgatgattt tgatgaagag aaaaaaatgt tggcgtcgat aaaaactgat attcaaaata
     1261 ttctgattcc tcgcaccatt aaaaaattac ctaaacgttt acataaactg ttcgacggaa
     1321 aatataaatt attcgttaac ccaacaggta aatttgtaat cggcggacca catggtgata
     1381 ccggacttac cggtcgtaaa atcattgttg acacctatgg tggtaaaggc gcgcatggtg
     1441 gtggtgcatt cagtggaaaa gatccttcga aagttgaccg ctccgcagca tacgccgtta
     1501 gacatattgc aaaaaacatg gtagccgccg gtatttgcga tgaagtttta gtgcaggttg
     1561 cctatgccat tggtgtagct gagccggtag gcttatatgt aaatacttat ggcactgcga
     1621 aagttaagat gcaagacgga gaaattgcaa aacgcattcg caaaatattt gatcttcgtc
     1681 catacgctat tgaacaacgc tttcaattgc gcacgccaat atatttagaa actgcagcct
     1741 atggtcacat gggtcgcgaa cccaaaacca tcacgaagcg atttgttgac ggtggaggaa
     1801 gaaagaaaag cattcgcgta accttattcc cttgggagaa attggattat gtagatgcga
     1861 tcaggaaggc gtttaagttg tgagtgggtg agtggtgaat ggtgtggtgg gtccagatcc
     1921 ctccgcaaac tccgccaact ccgcgattaa aatagggtga gtgggtgagt ggtgagcttg
     1981 cctcgccgat aggcgg
//
LOCUS       DAOQOU010000296         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_54362_length_1956_cov_5.752762, whole genome shotgun sequence.
ACCESSION   DAOQOU010000296 DAOQOU010000000
VERSION     DAOQOU010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54362_length_1956_cov_5.752762"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLM90_14620"
     CDS             <1..567
                     /locus_tag="PLM90_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMZ90668.1"
                     /translation="WDTKGITGVQGFLRKLWRLCVDEQGNMRVTNDEPSNEEFTILHR
                     TIKKATDDIERMSMNTVVSTFMICVNDLSAGKCYKRAIIQPLLVMTAPFAPFLSEELY
                     NKLGNDVSVHKASWPEVDERYLVRSSIPYAVQVNGKLRATVDIPTDMSDADIQATVLA
                     LDQVVKHMEGKSPKKVIVVKGKIVNVVV"
     gene            581..1120
                     /locus_tag="PLM90_14625"
     CDS             581..1120
                     /locus_tag="PLM90_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90669.1"
                     /translation="MKKSLLLVIICVCSSLASFAQEDDQLSDNQSNAYAQAQIMVGAF
                     EAGDYNRLLDYTYPAILVMGGGRDMLLSIISQMMSDMKAQGVVVDSARVGLPSQVYPA
                     GDELHALIPQYVWMSTSDTYIYSESTLLAVSDDNGSRWYFLDTRQLTPELKDSFFPDY
                     NSFLVIPPVKEVVVTPKVK"
     gene            1127..>1956
                     /locus_tag="PLM90_14630"
     CDS             1127..>1956
                     /locus_tag="PLM90_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HMZ90670.1"
                     /translation="MTEISQHIKLVECPRDAMQGLHDMIATDKKVEYINQLLRVGFDT
                     IDFGSFVSPKAIPQMADTREVLAQLDLSNTKSKLLAIVGNVRGAEDAAIFENIHYLGF
                     PFSISETFQQRNVNSSIAESVERVKEIKAICDGNGKQLMIYISMGFGNPYGDPWSADV
                     AASWVEKLVAMGIRHVSLADTIGVANPESIQYLFGHLIPAFPDVAFGAHLHTTPDAWL
                     EKIDAAYKSGCRRFDGAIKGFGGCPMAADDLTGNMATENIIQYFNHIGAELNINRSEF
                     L"
BASE COUNT          584 a          353 c          466 g          553 t
ORIGIN      
        1 tgggatacaa aaggtattac cggtgtgcaa gggttccttc gcaaactatg gcgtttatgt
       61 gtggatgagc agggaaatat gcgcgtaact aacgatgagc cttcaaatga agagtttaca
      121 attttacatc gaacgataaa aaaagcgacc gacgacattg agcgcatgag catgaatacc
      181 gttgtcagca cttttatgat ttgcgtgaat gatttaagtg ccggtaaatg ttataaacgt
      241 gccataattc aacctttgct tgtaatgacg gcgccatttg ctccattctt gtcggaagaa
      301 ttatacaata aattgggaaa tgatgtttca gtccacaagg cttcatggcc ggaggttgat
      361 gagcgttatt tggtgagaag cagcattcct tatgccgtgc aagtgaacgg aaaattgcgc
      421 gcaacggttg atatcccgac agatatgtct gacgctgata ttcaagctac cgttttggca
      481 ttggatcagg tggtaaaaca tatggaaggg aagtcaccga aaaaagtaat tgttgtaaaa
      541 ggcaagattg ttaatgtagt tgtttaaaaa attaatgggc atgaaaaaat cactattgct
      601 tgtaattatt tgtgtttgca gcagtctggc aagctttgcg caggaggatg atcaattatc
      661 cgacaaccaa agtaatgcgt atgctcaggc acagataatg gttggtgcat ttgaagcagg
      721 tgattataat agattgctag attatactta tcctgccatt ttagttatgg gtggaggaag
      781 agatatgttg ttgtctatca tatcacagat gatgagcgat atgaaagcgc aaggtgtggt
      841 ggtggattct gcccgagttg gattgccaag ccaggtttat cctgcaggcg acgaattgca
      901 tgcgttgatt ccgcaatatg tatggatgtc gacttccgat acttatattt acagtgaaag
      961 cacattgctt gcagtatcag atgataatgg aagcaggtgg tattttctcg acaccaggca
     1021 attaactccg gaattaaagg acagtttttt tcctgattat aatagttttc ttgtcatccc
     1081 tccggtaaaa gaagttgttg taacaccaaa agttaaataa aggaaagtga ccgaaatttc
     1141 tcaacatatt aaacttgtag aatgtcctcg cgatgcgatg caaggattgc atgatatgat
     1201 agcaaccgat aaaaaggttg aatacatcaa tcaattgctt cgcgttggat ttgataccat
     1261 tgatttcggc agtttcgttt cgccaaaggc aattcctcaa atggctgata cgcgtgaggt
     1321 attggcccaa cttgatctaa gcaatacgaa aagtaaattg cttgccatag taggaaatgt
     1381 gcgcggtgca gaagatgcag caattttcga aaatatacac tacctcggtt ttccattctc
     1441 tatttcagaa acatttcagc aacgcaatgt caattcgagc atagcagaaa gtgtggagcg
     1501 cgtaaaagaa atcaaagcaa tttgcgatgg taacggcaag caactcatga tatatatatc
     1561 aatgggtttc ggcaatcctt atggcgatcc atggagtgcc gatgtagcag cttcgtgggt
     1621 agagaaattg gtggcaatgg gcatacgcca tgtttcactg gcagatacca taggcgtggc
     1681 caatcccgag agtattcaat acttattcgg acatcttatt cctgcttttc ctgatgtagc
     1741 atttggcgct catttgcata caacaccgga tgcatggctc gaaaaaattg atgctgctta
     1801 caaaagcgga tgtcgtcgtt tcgatggcgc cataaagggt tttggcggtt gcccgatggc
     1861 agctgatgac cttacaggga acatggccac ggaaaatatt atacaatact tcaaccatat
     1921 cggcgcagaa ctcaatatca atagaagcga attcct
//
LOCUS       DAOQOU010000297         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_54553_length_1953_cov_3.814542, whole genome shotgun sequence.
ACCESSION   DAOQOU010000297 DAOQOU010000000
VERSION     DAOQOU010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54553_length_1953_cov_3.814542"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1953)
                     /gene="leuS"
                     /locus_tag="PLM90_14635"
     CDS             complement(<1..>1953)
                     /gene="leuS"
                     /locus_tag="PLM90_14635"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMZ90671.1"
                     /translation="KNIAYFREQLDRMGFCYDWNRGVKTSDPSYYKWTQWIFMQLFKH
                     WYDPFLNKAQPIADLEQMLEKEGSARLIEKDICDTLITADQWKSMSGDEKQKFLMNFR
                     LAFLDHAAIWWCEALGTVLANDEVKDGVSERGGHPVEKIRMRQWFLRITAYAERLLSG
                     LETVDFSDSMKEMQRNWIGKSEGALVDFKVEGSDNQIRIFTTRPDTIFGATFMVLAPE
                     HELVQQITSIDQQDAVSQYINYVKSRSERDRMSEVKKVTGQFTGAYAIHPFTGNKIPI
                     YIAEYVLAGYGTGAIMAVPSNDDRDAAFAEKFNLPIIPVVDQSNFPGAAREDKVGVMI
                     NSEMLNGLPVTEAILTMINAIATKGIGRRKINFRLRDAGFSRQRYWGEPFPVYYINDI
                     PYLLDESELPVILPQVESYKPAGAAQSPLSGVTDWVNLPNGARRETDTMPGYAGSSWY
                     YFRYMDVHNDKAFASKEALDYWQDIDLYIGGTEHAVGHLLYSRMWCKFLFDIGLVPKE
                     EPYKRLVNQGMIQGWSKIVYRIVGTNTFVSKQLKDQHETTELRVDIKMVDGDILDTEA
                     FKKWRTDFANAEFILEDGKYYCGTELEKMSKSKHNVVNPDDVINQYGADCFRLYEMFL
                     GPLDQGKPWDTKGITGVQGFLRKLWR"
BASE COUNT          540 a          475 c          345 g          593 t
ORIGIN      
        1 aacgccatag tttgcgaagg aacccttgca caccggtaat accttttgta tcccatggtt
       61 ttccctgatc cagcggacct aaaaacatct catacaaacg gaaacaatct gcaccatatt
      121 gattgatgac atcatcagga tttacaacat tgtgttttga tttggacatt ttttccaatt
      181 cagttccaca ataatattta ccgtcttcca agatgaattc tgcattggca aaatcagtgc
      241 gccatttttt aaatgcttca gtatctaaaa tatcgccgtc aaccattttt atatcgacac
      301 gcaattcggt tgtttcgtgc tgatccttta attgtttgct tacaaaagta ttggtgccaa
      361 cgatgcgata aacaattttc gaccaacctt gaatcatccc ttgattcacc aatcttttat
      421 atggttcttc ctttggcact aatccgatat cgaataaaaa tttgcaccac atgcgtgaat
      481 acaataagtg gccaacagcg tgttcagttc caccgatata cagatcaata tcctgccaat
      541 agtctaacgc ttctttcgat gcaaaggctt tgtcattatg cacatccata tatctgaaat
      601 aataccagct ggaacctgcg taaccgggca tggtatccgt ttctcgtctt gcaccgttgg
      661 gtaaattcac ccaatcggta acaccactta atggcgactg tgccgcaccg gcaggtttat
      721 aactttctac ttgtggcaat attacaggca actcactttc atcgagcaaa taagggatat
      781 cgttgatata ataaaccgga aatggttcac cccaatatcg ctgacgagaa aaccctgcat
      841 cgcgcaagcg gaaattaatt tttcttctgc caataccttt tgttgcgatg gcattaatca
      901 ttgtcaaaat agcttctgta accggcaatc cgttcagcat ttctgaattg atcattaccc
      961 caaccttatc ttcacgcgct gccccgggaa aattactttg gtcgactaca ggaataatcg
     1021 gcaaattaaa tttctctgca aatgcggcat ctctgtcatc attgctaggc actgccatga
     1081 tagcgccggt tccatatcct gcaagcacat attcggcaat ataaatcgga attttatttc
     1141 cggtaaacgg atgaatcgca taagcaccgg tgaattgtcc cgtcactttt tttacttctg
     1201 acatgcgatc tctttcactg cgacttttca cataattgat atactgagat acagcatctt
     1261 gctgatcgat ggaagtaatc tgctgcacaa gttcatgttc aggtgccaac accataaatg
     1321 ttgcaccgaa aatggtatcg ggacgagtgg taaaaatgcg gatttgatta tcacttcctt
     1381 caaccttaaa atcaactaat gctccttcac ttttaccgat ccagttgcgt tgcatttctt
     1441 tcatgctatc gctgaaatca actgtttcca ggcctgacaa taagcgttcg gcataagctg
     1501 ttatcctcag gaaccactga cgcatgcgga ttttctccac cggatgacct ccacgttcac
     1561 taacgccatc tttcacttca tcatttgcca acactgttcc caatgcttcg caccaccaaa
     1621 tggcggcatg atctaaaaat gccaggcgga aattcatcag aaacttctgc ttctcatcgc
     1681 ccgacataga tttccattgg tcggcagtta taagtgtatc acaaatgtcc ttttcaatta
     1741 gtcgtgcact accctctttt tccaacattt gttcaagatc tgcaattggt tgcgctttat
     1801 tcaggaatgg atcgtaccaa tgcttgaaca actgcataaa gatccattgg gtccatttgt
     1861 aataggaagg gtcagaagtt ttcactcccc tgttccaatc gtaacaaaat cccatgcgat
     1921 cgagctgctc gcggaaatag gcaatatttt tct
//
LOCUS       DAOQOU010000298         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_56344_length_1921_cov_7.572883, whole genome shotgun sequence.
ACCESSION   DAOQOU010000298 DAOQOU010000000
VERSION     DAOQOU010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56344_length_1921_cov_7.572883"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..714)
                     /locus_tag="PLM90_14640"
     CDS             complement(175..714)
                     /locus_tag="PLM90_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ90672.1"
                     /translation="MVCARCKMVVSGVLTELGYKPVVVELGEVEISGDVDSEGKDKIN
                     NRLKQFGFELIDDKRSKIIDRVKTLVVDIVHNKDGDLTTNLSDLLSNELHHEYNYISN
                     LFSSVEGTTIEKYFIAQKIERVKELIIYDELSLSEISFRMHYSSVAHLSSQFKKVTGL
                     TPSEFKAINGPRRTNIDEV"
     gene            complement(770..>1921)
                     /locus_tag="PLM90_14645"
     CDS             complement(770..>1921)
                     /locus_tag="PLM90_14645"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HMZ90673.1"
                     /translation="IAIATEMDTLPMVNATVDAVADLPAQVVRLRLLNGSSMRTFHIG
                     FDNNQLFYVIAGDGGLLSEPVALNRLILAPGERYEILVDLSGMESEVLTMMSFGSTLP
                     SGMYGAANPSGMGMGTITGYDANPLNGSDFSLLKIQVIDQTADPVTEIPSSLTPPITT
                     PAYTATRVFNLEPESMGMDNMVMGPFVINGASFNMDIVNEIVPINTIEKWRINNNTGI
                     AHPFHIHEGQFIIDNINGAEPDPVDRGMQDVVYIPAGQYAEVIKTFSDFADDQVPYMY
                     HCHMLHHEDDGMMGSFTVVAQDEVINDHIVPPLIYPNPVASNSTFSIVTEGEFERMEI
                     YNSMGEIVYLDDFHQQAVNAYTIDGLPSGVFIIKLINGKNDNATTFIVQ"
BASE COUNT          588 a          417 c          315 g          601 t
ORIGIN      
        1 atttgacaag ttcgcgatca atcagcagtg cacaatgttc actttccatg ccttcaatag
       61 gcaaaatgat cggctcttca atttcgtttg ccatagttgt atcgatttag gatacaaagc
      121 tgaggcgaat tccgggtcca agtgttacac tatttctata atgaattaca tcatttacac
      181 ttcatcgata tttgttcttc tgggcccgtt tatagcttta aactcacttg gtgtaagtcc
      241 agtaactttc ttgaattgac tacttaaatg tgcaacagat gagtaatgca ttcggaagga
      301 tatctcgctt aatgaaagct catcataaat aatcaattct tttacccgtt caatcttttg
      361 tgcaataaaa tacttttcaa tggtagttcc ttcaactgat gaaaataaat tactgatata
      421 attatattca tgatgaagtt cattactcag caaatctgac aagttagtag ttaagtctcc
      481 atctttatta tgcactatat ctaccaccaa agtttttact ctatcgatta tttttgagcg
      541 tttgtcatct atcaattcaa atccgaactg cttcaatcga ttgtttattt tatcctttcc
      601 ttccgaatca acatcacccg atatctccac ctcccccaat tcaacaacaa caggtttata
      661 ccccaactca gtcaatactc cggaaaccac cattttacat ctggcacaaa ccatattccg
      721 aatatatatt ttcattatgg cgtcgtttat aataatctaa tttattatat cattgcacta
      781 taaaagtagt ggcattatca tttttaccat taatcaattt aatgatgaac actcccgaag
      841 gcagaccatc gatagtatag gcattaacag cctgctgatg aaaatcatct aaatagacaa
      901 tttcacccat agaattataa atttccatcc tttcaaattc accttctgta actattgaaa
      961 acgttgagtt tgatgcaact ggattaggat aaatcaatgg tggcacgata tggtcattaa
     1021 tgacttcatc ctgtgctaca accgtaaaac ttcccatcat tccgtcgtcc tcatggtgca
     1081 acatgtgaca gtgatacata tatggcactt gatcatcggc aaaatcgctg aaggttttaa
     1141 ttacttcagc atattgtccg gcgggtatat aaacaacatc ctgcataccc cggtcaacag
     1201 ggtctggctc agcgccatta atattatcaa taataaactg accttcatga atatgaaagg
     1261 gatgtgctat tccggtatta ttattaatgc gccatttttc aatagtattg ataggaacta
     1321 tttcatttac aatatccata ttaaatgatg ctccatttat tacaaaaggc cccatcacca
     1381 tgttatccat ccccattgat tcaggttcta aattaaaaac tcgggttgcc gtataagcag
     1441 gcgttgtaat cggcggagtc aatgacgatg gaatttcagt gaccggatct gcagtttgat
     1501 caatgacctg aattttaagt agtgaaaaat ctgaaccatt caacggattc gcatcataac
     1561 cggtaatagt tcccataccc ataccggagg ggtttgcagc gccgtacatg cctgatggaa
     1621 gcgtagaacc aaacgacatc attgtcaaaa cttcagattc cattccactg aggtcaacca
     1681 atatttcata gcgctcgccg ggagcaagaa ttaatctatt tagagccaca ggttccgata
     1741 ataatccacc atcgccggca attacataaa ataactggtt attgtcaaac ccaatatgaa
     1801 aggtgcgcat gctggatcca ttcaataatc gcaatcgcac tacctgagca gggaggtctg
     1861 ccacggcgtc aacagttgca tttaccattg gcagcgtatc catttctgtt gcaatggcta
     1921 t
//
LOCUS       DAOQOU010000299         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_57716_length_1897_cov_5.299674, whole genome shotgun sequence.
ACCESSION   DAOQOU010000299 DAOQOU010000000
VERSION     DAOQOU010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57716_length_1897_cov_5.299674"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /gene="lon"
                     /locus_tag="PLM90_14650"
     CDS             <1..1530
                     /gene="lon"
                     /locus_tag="PLM90_14650"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMZ90674.1"
                     /translation="KLQRLNPNAAEYGVITNYIELLLELPWNEYTTDNFDLKHAQDVL
                     DQDHYSLEKVKDRLIEYLSVLKLKGDLKSPIICLVGPPGVGKTSLGQSVARALGRKYV
                     RMSLGGLHDESEIRGHRKTYIGAMPGRVLQSIKRVKSSNPVFILDEIDKVGTDFRGDP
                     SSALLEVLDPEQNSTFYDNYLEIEYDLSKVLFIATANNINSIQPALRDRMEIIYLSGY
                     STEEKIEIAKRHLLPKQKSEHGLKPKDIKISDAGLLTIVENYTRESGVRDLDRQIASV
                     MRRIAKYVATNEAYAKTLQPADIIKILGPEKFDKESIFDNMPAGVVVGLAWTPVGGDV
                     LFIETSLSKGKGTLQMTGNLGDVMKESASTALAFIRANADKLGIAGEAFEQSAIHIHV
                     PEGAIPKDGPSAGITMLTALTSAFTGRRVKPFFAMTGEITLRGKVLPVGGIKEKVLAA
                     RRAGVKNIIMCANNKKDVAEINPTFIKGVKFHYVESMQEVLDLALEPLKPAKAAKVKR
                     K"
     gene            1616..>1897
                     /locus_tag="PLM90_14655"
     CDS             1616..>1897
                     /locus_tag="PLM90_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90675.1"
                     /translation="MKRILFISIFFSAISSVYGQFGGSYTYAYLQLPPSARISGLGGL
                     NISTYDNDIAMAWQNPALLNEGMDGDISANHAFMPSGISHGGVMYGKSLK"
BASE COUNT          564 a          370 c          451 g          512 t
ORIGIN      
        1 aaactgcagc gcttaaatcc caatgccgct gaatatggcg tcatcaccaa ttatattgaa
       61 ttattgcttg agctgccgtg gaatgaatat accaccgata actttgatct gaaacatgcg
      121 caagatgtgc tcgatcagga ccattatagt ctggaaaagg ttaaggatag attaatagaa
      181 tatctttctg ttttaaaact gaaaggtgat ctgaaatccc ctatcatttg tttggttggg
      241 cctccggggg taggaaaaac ttcattggga cagtcggtag cgcgtgccct gggcagaaaa
      301 tatgtgcgca tgagtttggg tggattgcat gatgaaagcg agatacgcgg tcatcgaaaa
      361 acatacatcg gtgccatgcc gggacgtgta ttgcagtcta tcaaacgcgt aaaatcatcg
      421 aaccctgttt tcattcttga tgaaatagat aaagtaggca cagattttcg tggcgatcca
      481 tccagtgcat tattagaagt actagatccg gaacaaaaca gcacctttta cgataattat
      541 ctggaaatag aatacgacct ttccaaggta ttatttattg ctaccgcgaa taacatcaac
      601 agcatacaac ctgccttgcg cgataggatg gagattattt atctcagcgg ttattctacc
      661 gaagagaaaa tagaaattgc caagcgacat ttgttgccga agcaaaaaag cgaacacgga
      721 ttaaaaccaa aggatattaa aatatctgat gccggtttgt tgaccattgt agaaaattat
      781 acacgtgaaa gcggtgtgcg tgatctcgac aggcagattg cttccgtgat gcgtcgcatt
      841 gcaaaatatg tggctacaaa tgaagcatat gcaaaaacat tgcaacctgc agatatcatt
      901 aaaattttag gtcctgaaaa attcgataag gaaagtatct tcgataatat gcctgccggt
      961 gtggttgtcg gacttgcatg gacgcctgta ggaggcgatg tattatttat cgaaacatcg
     1021 cttagcaagg gcaagggcac tttgcagatg acaggaaatt tgggtgatgt gatgaaagaa
     1081 tctgcttcta cagcgctggc atttattcgc gctaatgccg acaagttggg tatcgccggt
     1141 gaagcatttg aacagagtgc cattcacatt cacgtacctg aaggtgccat cccgaaagat
     1201 ggtccgagtg ccgggattac catgcttacg gcattgacat cagcatttac aggccgtcgc
     1261 gtgaaaccat tttttgccat gacaggtgaa ataactttgc gcggaaaggt attgcctgtt
     1321 ggcggtatta aagaaaaagt gcttgccgca agaagagccg gcgtgaaaaa catcattatg
     1381 tgtgccaaca ataagaagga tgttgcggaa atcaatccca cctttattaa aggcgtgaag
     1441 ttccactatg tggaaagcat gcaggaagta ttggatctcg cactcgagcc gcttaaacct
     1501 gcaaaggctg cgaaggttaa gcgcaaatga agtcagactt ctcaaacaca taaatctcat
     1561 taacttagtc ccgtaaaatt ttttgcggga cttttcgtta agcacgcagt cgaacatgaa
     1621 gcggatactt ttcatcagca tattttttag cgcgatatcg tctgtttacg gccagtttgg
     1681 gggcagttat acctatgcct acctgcaatt accaccatcg gcgaggatta gtggtttggg
     1741 tggcttaaac atttctacct acgataatga cattgccatg gcatggcaga atcctgcact
     1801 attaaatgag gggatggatg gtgatatttc ggccaatcat gcttttatgc cttcaggaat
     1861 atcgcatggt ggtgtgatgt atggcaaatc tttaaaa
//
LOCUS       DAOQOU010000300         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_58483_length_1884_cov_5.373975, whole genome shotgun sequence.
ACCESSION   DAOQOU010000300 DAOQOU010000000
VERSION     DAOQOU010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58483_length_1884_cov_5.373975"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1258)
                     /locus_tag="PLM90_14660"
     CDS             complement(104..1258)
                     /locus_tag="PLM90_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90676.1"
                     /translation="MKTSTLLLIAALIAVQSVSFAQTNTRSTAAVANPSVQGMALSPE
                     VAAKMLRIELLKTGLFQVWDEFDMDEALRNDAKYQTGCYGVMCLSELGNELNVNYVFS
                     GSIDKLADRIVINIKVVDVKNVSVQTSLTREFSREDEAIQRMFESMLCEMYQIPFDPE
                     VLATLQFNNGAVVKESYTKINNTGPRMGAAYMMGGLYEFANRPESQGGLDIAPVMSLI
                     GYQFEWQYVGTENFSALVECVVNFSGMEQGRFIPSVDILNGFRFGTAGWEFGFGPGFG
                     IITTSQGFFDEQNTFGKGADYYFSEKDWESYSFAQYGEVVDVSEINPNYSFSENFDAR
                     GDAKLNTFWIFAAGRTFRAGSLNIPVNAFYTLMDDGGYAGVSFGFNVVKK"
     gene            1466..>1884
                     /gene="ribD"
                     /locus_tag="PLM90_14665"
     CDS             1466..>1884
                     /gene="ribD"
                     /locus_tag="PLM90_14665"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HMZ90677.1"
                     /translation="MHEHYMERCIDLASRGLGFTAPNPCVGCVIVHDERVLGEGWHKQ
                     YGAAHAEVEAFASVHGSDVALLPESTVYVSLEPCNHHGKTPPCTDLLIRHGVKRVVIG
                     SMDPNPIVAGAGIQRLRDAGIEVISGVLTDACRKLNAP"
BASE COUNT          498 a          434 c          441 g          511 t
ORIGIN      
        1 caacaggcac tgaccttgtt gaaaaaatga tgcacacaga taaaggtaca aacaataaag
       61 cctcccgaat aggaggcttt attgtttgta ttgttgtcag taactatttc ttcactacat
      121 taaacccaaa gctcacacct gcataacctc cgtcatccat taatgtatag aaagcattta
      181 caggaatatt gagtgaacct gcgcggaagg tgcggcctgc tgcgaatatc cagaaggtat
      241 tgagtttggc atcgccgcgg gcatcgaaat tttcggagaa ggaataattt ggattaattt
      301 ccgaaacatc caccacttct ccgtattgcg cgaaactgta actttcccaa tctttttcgc
      361 tgaagtaata gtcggctcct ttgccaaaag tattttgttc gtcaaagaaa ccctgtgaag
      421 tggttatgat accaaaaccg ggaccaaatc cgaattccca acctgcggta ccgaaacgga
      481 aaccattcaa tatatctact gaaggaataa aacgtccttg ttccattcct gaaaagttta
      541 caacgcattc taccagtgca gagaaatttt ctgtgcccac atattgccat tcgaactgat
      601 aaccgatcag actcatcact ggtgcgatat ctaaaccacc ctgactttca ggacgattgg
      661 caaattcgta caatccgccc atcatataag cagcacccat acgtggtccg gtgttgttga
      721 tcttggtgta actttccttt acgactgcgc cattgttgaa ttgtaaggtg gccagcactt
      781 caggatcgaa tggtatttga tacatttcgc ataacataga ttcgaacata cgctggatgg
      841 cttcatcttc tctggagaat tcgcgggtaa gtgaagtttg cacgcttaca ttttttacat
      901 ccactacttt gatattgatc acaatgcggt cggccagttt gtcgatgctg ccactaaata
      961 cgtaattgac attgagttcg ttgccgagct cacttaagca catcacgcca taacaaccgg
     1021 tttggtattt ggcatcgttg cgcagggctt catccatgtc gaattcatcc caaacctgaa
     1081 ataagcctgt ttttaataat tcaatgcgca acatcttcgc ggctacttca ggggagagtg
     1141 ccatgccttg cacagaagga ttagcaacag cagcagtgga acgtgtattt gtctgggcga
     1201 aggaaactga ttgcacagct ataagtgcgg cgatcagtaa aagtgttgaa gttttcattg
     1261 atttaagttt atggggcaaa tgtatccgga tagataaggc gactcaaatg gtgtagcgca
     1321 acttgatcgt accggtattt gcgattttta ctaaaacacc ggacccccag tctaaaaacc
     1381 gcaacttttg cctttccgct cgcgggattt tggcagaaag tcgtaacggg catgtgttgc
     1441 cggcttgatt ctctaccttt gcagcatgca cgaacactac atggaacgat gtatcgacct
     1501 ggcttccaga ggcttgggct ttactgctcc aaacccttgt gtaggctgcg tgatcgtgca
     1561 tgacgaacgc gtgttggggg agggctggca caagcaatat ggtgctgcac atgcggaagt
     1621 ggaggccttt gcctccgtgc atggttcaga tgtggctctg ttgcctgaat caacggtata
     1681 tgtgagcctt gagccctgta atcaccatgg taaaacgcca ccctgtaccg atctgctcat
     1741 caggcatggg gtaaagcgcg tggtgatcgg ttctatggat ccgaatccga tcgtggcagg
     1801 tgcagggata cagcggttgc gtgatgccgg catagaggtg atctccggtg tattgacaga
     1861 tgcctgcaga aagctcaatg cccc
//
LOCUS       DAOQOU010000301         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_61825_length_1828_cov_6.034969, whole genome shotgun sequence.
ACCESSION   DAOQOU010000301 DAOQOU010000000
VERSION     DAOQOU010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61825_length_1828_cov_6.034969"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLM90_14670"
     CDS             complement(<1..238)
                     /locus_tag="PLM90_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HMZ90678.1"
                     /translation="MAPNRRDFLKQTGLASAGILVPSFIKASVPFLPGDVRPKVLVMI
                     QFTGGNDGLNCIVPIGNDLYHKARPNIGYKKEELI"
     gene            complement(240..1634)
                     /locus_tag="PLM90_14675"
     CDS             complement(240..1634)
                     /locus_tag="PLM90_14675"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HMZ90679.1"
                     /translation="MASQQRHVQHLYLRAGFGESPAIVKQRSAKEIKQLVDDLFTESK
                     QYRNLTYLENPAKGKEVSNLKVLLLILKSQEQMRELNNVWMGMLASTKAVLREKMTLF
                     WHDHFATSTQFAYLMQLQNNTLRKHALGRFDKLLHAIAKDPAMIIYLNNQQNKKDAPN
                     ENFAREVMELFTLGEGNKYTEADIKEAARAFTGWTVNKKGEFEFVAKDHDDESKTFLG
                     RAGNFNGEDIINILLEEKQTAKYVTTKIYKEFVNDNVNDAHVEALSTIFFDSGYDISA
                     LMRNIFTADWFYADENMGSLIASPVDLITRFRRLIKMEFKEEKGQFNTQKILGQVLFF
                     PPNVAGWKGGRNWVDSSTLMLRMQIPTRIIRGGGFNVRPKPEFEDAPEDEMTLKTSKK
                     ADVRSDWSGVVNYFKDVPTDQLTESVIETFIQSSSDHIDRNIIAQYVDNSSDEKRILS
                     TIGVVMSLPEFQLI"
BASE COUNT          498 a          406 c          359 g          565 t
ORIGIN      
        1 tgatgagttc ttctttttta taacctatat tcggacgagc cttatgatac aggtcgttgc
       61 cgatgggaac gatgcaattc aatccatcat tccctccggt aaactgtatc atcaccaata
      121 cttttggtct tacatctccc ggcagaaatg gcacggaagc cttaatgaat gaaggcacca
      181 atatccctgc tgatgccaat cctgtttgtt tgagaaaatc tcttctattt ggtgccatat
      241 cagatgagtt gaaattcagg taaagacatc accacgccaa tggtgcttaa aatgcgtttt
      301 tcgtcactgc tattatcgac atattgggca ataatatttc tgtcgatatg gtcgctggag
      361 gattgaataa atgtttcgat aacgctctca gtcagttgat cggtggggac atccttaaaa
      421 taatttacta ctcccgacca atcagagcgc acatctgctt ttttggaggt tttcaatgtc
      481 atctcatctt caggcgcatc ttcaaattcc ggtttgggtc gaacattaaa tccgccacct
      541 ctgataatac gtgtaggaat ttgcattctc aacatgagcg tagagctgtc gacccaattc
      601 cttccgccct tccaacctgc cacattcgga ggaaaaaata atacctgtcc taatattttt
      661 tgtgtattaa actgaccctt ctcttcttta aattccatct ttatcaacct acgaaagcgc
      721 gtaattaaat caacaggtga tgcgataagg gaccccatgt tttcatcggc ataaaaccaa
      781 tctgccgtaa aaatattacg catcaacgct gaaatatcat aaccactatc gaaaaatatt
      841 gtcgacaatg cctctacatg cgcatcattc acattatcat ttacaaactc cttataaatt
      901 ttggtagtaa catattttgc tgtttgcttt tcttcaagca aaatattaat tatatcctcg
      961 ccattgaaat ttcctgccct tccaagaaat gttttgcttt catcgtcgtg atcctttgca
     1021 acaaattcaa attcaccctt tttattcacc gtccaccccg taaatgctct ggccgcttct
     1081 ttgatatctg cctcagtgta tttgtttcct tcacccaacg taaacaactc cattacctca
     1141 cgtgcaaaat tttcgttggg tgcatctttt ttgttctgct gattattcag ataaataatc
     1201 attgcaggat ctttagcaat ggcgtgtaac agcttatcga atctaccgag tgcatgtttt
     1261 cgcagcgtat tattttgcaa ctgcatcaaa taagcaaact gcgtagaggt ggcaaaatga
     1321 tcgtgccaga ataaggtcat tttttcgcgc agcacagctt tcgtcgatgc aagcataccc
     1381 atccacacat tatttaattc gcgcatctgc tcttgtgatt tcaggatgag caacaatact
     1441 ttcaggtttg aaacttcctt cccttttgca ggattttcga gatacgtgag attgcgatat
     1501 tgcttacttt cagtaaataa atcatcaacc agttgcttaa tttctttagc cgagcgctgc
     1561 ttgacaatag cgggtgattc accaaagccg gctcttaaat acaaatgctg gacgtgacgt
     1621 tgctgggaag ccataagcgg gatttgggaa gtaatataca aagaagatgc cagagtggtg
     1681 aggggtgagg ggtgaggggt gagggggcga acgggtgaat tgtgatctta ccacgccgaa
     1741 aagcggttcg tgacacctcc gcaaactccg ctaactccgt gattgaaaaa aggtgagtgg
     1801 gtgagtggtg aatcgtgagt cgtgattc
//
LOCUS       DAOQOU010000302         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_63616_length_1802_cov_4.614196, whole genome shotgun sequence.
ACCESSION   DAOQOU010000302 DAOQOU010000000
VERSION     DAOQOU010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63616_length_1802_cov_4.614196"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLM90_14680"
     CDS             <1..269
                     /locus_tag="PLM90_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90680.1"
                     /translation="LQSQAYLPATVQKQLVDATTRAVNDPQFLRVDYTNKTVQLSKIF
                     EWYADDFNKTSGSVIQFINTYRSAKISDSYTLSYYEYDWSLNKL"
     gene            complement(280..807)
                     /locus_tag="PLM90_14685"
     CDS             complement(280..807)
                     /locus_tag="PLM90_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90681.1"
                     /translation="METLGVGSRIKHPEHGMGVIIHVHKRAYDVCYIDKGIKAMLRDT
                     HVEVIEAMPSEYDVSFTEAEDALIKILREWSDVTEVIHIAEKWEGGTLLMQPKDKSMQ
                     GKEVPIETFFHKIVMLRDRLRVLEQKVNAHNKLTDSEKVEMQQYITRIYGSLTTFNVL
                     FKLKADYFVGDKKEE"
     gene            complement(939..>1802)
                     /locus_tag="PLM90_14690"
     CDS             complement(939..>1802)
                     /locus_tag="PLM90_14690"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90682.1"
                     /translation="LSLAAPQYITAVSPPGIALGDFDSDGKLDVITMVDGMGWNLAEA
                     FRNTTATPGDITFAAPQTFTPVSDKVIVDDFDGDGADDFAGVNDASNHVSVWVHDNML
                     LANHQPQVTVYCGEDANAWIEFTVDAKSTAEYFIVERTVDGQHFEQVAKISAQAGSAS
                     HTYEVTDPHPAWETVYYRILTFDATEKQVVSSLYPLSPCNTLVTDFTCVFPNPVGDVM
                     NFKFSITAQTEMHMQVLDMSMKKKIQKVETVTNGTRTYSLDVRDLPKGSYIFLLRFGN
                     LPPHLCQFEKL"
BASE COUNT          506 a          403 c          364 g          529 t
ORIGIN      
        1 aattacaatc gcaagcctat ttaccggcaa ctgttcaaaa gcaactggta gatgccacaa
       61 cgcgggctgt aaatgatccg cagtttttaa gagtagatta taccaataaa accgttcagc
      121 tttcaaaaat cttcgaatgg tatgccgatg atttcaataa aacttcaggc agtgtgatac
      181 agtttatcaa tacctatcgc agtgcaaaaa tttccgacag ttatacattg tcgtactacg
      241 aatacgactg gagtttgaat aagctctagc acttattttc tattcctctt tcttatcgcc
      301 gacaaagtaa tcagccttta atttaaacaa cacattaaag gtggtgagcg aaccgtagat
      361 gcgagtgata tattgttgca tttctacttt ctcgctatca gtgagtttat tgtgtgcatt
      421 tactttttgc tccagcacac gcagtctgtc gcgcagcatc acaattttat ggaagaaggt
      481 ttctatcggc acttcttttc cctgcattga tttatcctta ggctgcatca ggagtgtgcc
      541 gccttcccat ttttcggcga tatgaattac ttcagtaaca tcgctccatt cgcgtaatat
      601 tttaatcagc gcatcttccg cttccgtaaa gctgacatca tattcagaag gcattgcttc
      661 gatgacttcg acatgagtat cgcgaagcat tgctttaatc cctttatcga tatagcaaac
      721 atcatatgcg cgcttatgca cgtgaatgat aacgcccatg ccatgttcag gatgttttat
      781 tctactgccg actccaagtg tttccatata ccattcattt ttcgcaataa tactgcatag
      841 caggcagatt tatatatttc tgtagacaca aaaaaaagcc cgtttgccac gacagcaaac
      901 gggtatcggt gccgaacacg acttcagcac ttcacacact acaacttttc aaattggcaa
      961 agatgcggag gcaaattgcc aaatctcagg aggaatatat aagaaccttt gggcaggtca
     1021 cgcacgtcta aagaatatgt tcttgttccg ttagtgacag tttccacttt ttgaattttt
     1081 ttcttcatcg acatatccaa aacctgcatg tgcatttcag tttgagcagt aatgctgaac
     1141 ttgaagttca tcacatcgcc aaccggattg gggaacacac aggtgaaatc tgttaccagt
     1201 gtgttgcatg ggcttaatgg atacaaagat gatacaactt gcttttctgt tgcatcaaat
     1261 gtaagaattc gataataaac tgtttcccac gccggatgtg gatcagtgac ttcataggta
     1321 tgggaagcgg atccggcttg tgcagaaatt tttgccactt gttcaaagtg ctgtccatca
     1381 acagtgcgct ctacaatgaa atactcggca gtagattttg catcaaccgt gaactcaatc
     1441 caggcattcg catcttcacc acaatataca gtgacctgag gttgatgatt agcaagaagc
     1501 atattgtcgt gtacccaaac ggatacatga ttggatgcat catttacacc cgcaaaatca
     1561 tcggcgccat cgccatcaaa atcatctaca attactttat cgctgacagg tgtaaaggtt
     1621 tgcggagcgg cgaatgtgat gtcgccgggt gttgctgtag tgtttctgaa agcttctgcg
     1681 aggttccacc ccataccatc caccatagtg atcacatcca atttgccatc tgaatcgaaa
     1741 tcgcccagtg cgatacccgg aggggaaacg gcggtgatgt attgtggagc tgccaaggat
     1801 aa
//
LOCUS       DAOQOU010000303         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_72049_length_1687_cov_4.631740, whole genome shotgun sequence.
ACCESSION   DAOQOU010000303 DAOQOU010000000
VERSION     DAOQOU010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72049_length_1687_cov_4.631740"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLM90_14695"
     CDS             complement(<1..830)
                     /locus_tag="PLM90_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90683.1"
                     /translation="MNNNRLLYHLIGSLSKAEKRYVSRYAAFNRDKSRLLKLFNFINE
                     HGITSDEDLRKSNKNATLLRDIEVSKVHLTQLILVSLRQYHRQEGGERRLRGLMDDAD
                     ILWRKSQYQACAQTLKKVKRLAKSLGYYTYVLEAVYMERNMMGTHPAFPLTEETVDIA
                     NREEKEALEALQTEFKFRNLAARSAIIHKRFGNSRSETDFAEITALAAMPEYDVSEAT
                     TLRSKRFYHHSMLTYYSSIGNLERAVMHNREILETYNAPELIREHAAGFIIAHNNQAL
                     "
     gene            990..>1687
                     /locus_tag="PLM90_14700"
     CDS             990..>1687
                     /locus_tag="PLM90_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMZ90684.1"
                     /translation="MTPIKSFVISMFLVGAAWLSAAPAYGQFSLLEIAEGPMASIGSA
                     AFGIESADLDGDGKPDIVSSFYGSGNVNVFRNISTGTGPMQTIFDAPIDITYGTGAAY
                     NVAIADLNKDYKPEILIANTALAEVTILPNHSTPGVINFGTAVHIPCPETPYALTACD
                     LNMDGYSDLIATCNSKLAIYRNTTAAVGGDISFDSYSELIEATTWRDVMCADMDGDAR
                     VDIVAGGSDRVAIF"
BASE COUNT          445 a          395 c          362 g          485 t
ORIGIN      
        1 aataatgcct gattgttatg cgcaatgatg aatcccgctg catgttcgcg aattaattcc
       61 ggggcattat aagtttcaag gatttcccga ttgtgcatca ctgcgcgctc aagattgccg
      121 atgctggaat aataggtgag catgctatga tgataaaagc gtttggagcg aagggtagta
      181 gcctccgaaa catcatactc gggcattgcg gcaagtgcgg taatctccgc aaaatctgtc
      241 tcacttcgcg agtttccaaa gcgcttatgt atgatagccg aacgcgcagc aagattccgg
      301 aatttaaatt ctgtttggag tgcctccagc gcttcttttt cttctctatt ggcaatatca
      361 acagtttctt cagtcaaagg aaacgccgga tgtgtgccca tcatatttcg ctccatgtaa
      421 accgcttcga gcacataggt ataataccct aaactttttg caagtcgctt tacctttttg
      481 agtgtttgtg cgcatgcctg atattgcgat ttccgccata aaatatctgc atcatccatc
      541 aacccgcgca gccgacgctc gccaccttcc tgccggtgat actggcgcaa cgataccaat
      601 atcaactgcg tcagatgcac cttcgaaacc tctatatcgc gcagcaaggt tgcattttta
      661 ttcgatttcc tgaggtcttc atcactggta atgccatgtt cattgatgaa gttgaaaagc
      721 ttcaacaggc ggcttttatc tctgttaaat gccgcatacc gactcacata tctcttttca
      781 gccttgctca agctgcctat gagatggtaa aggagtcgat tgttattcat agaaacttaa
      841 tcttgaacta tcaaaaatag ttaaaattag tgtataataa atattacaat gcaccacagg
      901 tacttaatat ctgtgtacat ttttgcttgc ctgaacctgc ctgatagccg aattttgaag
      961 catcaagcag aaaacaaaag cataatccaa tgaccccaat caaaagcttc gtgataagca
     1021 tgttcctggt aggagcggcc tggctgtctg ccgctcccgc ctacggacaa ttttccttac
     1081 tggaaatagc ggaaggccct atggcttcaa taggctctgc agccttcgga attgagagcg
     1141 ctgatttgga tggcgatgga aagcctgata tcgttagttc gttttatggc tccggaaacg
     1201 tcaatgtctt tcgcaatatc agcacaggaa caggccctat gcaaaccata ttcgatgcac
     1261 ccatcgatat cacttatggc acgggggctg cttataatgt ggctattgcc gatctcaaca
     1321 aagattataa acctgaaatt ctcatcgcaa atacagccct tgctgaagta acaatattgc
     1381 ctaatcattc cactccgggt gtgataaact tcggcacagc ggttcacatt ccttgccctg
     1441 aaacacctta tgcacttact gcctgcgacc tcaatatgga cggatatagc gatcttattg
     1501 ccacatgtaa ctcgaaattg gcaatttatc gaaataccac agcagcagtt ggcggtgata
     1561 tttcttttga ctcttattct gagcttatag aagctactac gtggcgcgat gtgatgtgtg
     1621 ccgatatgga tggcgatgcc agagtggata tcgttgccgg aggatctgat cgcgtggcta
     1681 tttttca
//
LOCUS       DAOQOU010000304         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0462
            NODE_73238_length_1673_cov_5.335600, whole genome shotgun sequence.
ACCESSION   DAOQOU010000304 DAOQOU010000000
VERSION     DAOQOU010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810094
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:11:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73238_length_1673_cov_5.335600"
                     /isolate="AMR_MDS_0462"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLM90_14705"
     CDS             complement(<1..484)
                     /locus_tag="PLM90_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ90685.1"
                     /translation="MKRSLYIVMFLITGILFAGCPYSAEIALDERPNIPVDKAMLGKW
                     ELRSSSDYTYVITAKNDFVYSIMKKSTSDGSETVYEGFLSDINGKKFLNIYETGSTTP
                     TYYFYLFTQTGNDRFKLDPVTDNIDETFTNSRDMRNYFAKNMDVSFFFDKDADEYLRS
                     N"
     gene            552..1580
                     /locus_tag="PLM90_14710"
     CDS             552..1580
                     /locus_tag="PLM90_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ90686.1"
                     /translation="MIWTAMGATPSGESLRRIASMPNYTKGQFQNIEHTPVMSEDYHM
                     GKVMMDMMKRNSARTPATPLPNVKTDLLHLENNALVWFGHSAYLLKINGKHILVDPVF
                     SGHASPVPFMIKAFKGSNSYGVADMPEIDMLVITHDHYDHLDYETVVALKSKVKEVVC
                     SLGVAAHLIKWGYQESHIHELYWHESKTVFENFTFTAEPARHFSGRGITRNKSLWCAF
                     ALQTPELTVFIGGDSGYGKHFKEIGTRYNGFDIALLECGQYNPAWKYIHMMPEETVMA
                     AQDIQAKIFVPVHWGKFALSLHTWQEPADRVVAAAQRAEMPYATPKIGEAYVIGAGGQ
                     THHWWQMR"
BASE COUNT          483 a          334 c          374 g          482 t
ORIGIN      
        1 aattcgacct caaatattca tcggcatcct tatcaaaaaa gaaacttaca tccatattct
       61 tggcgaaata attgcgcata tcgcgggagt tggtgaatgt ttcatcaata ttatcagtca
      121 ccggatcaag cttaaaacgg tcattacctg tctgcgtgaa aagataaaag taatacgtag
      181 gtgttgtact tccggtttcg taaatattca aaaatttctt tccattaata tccgacaaaa
      241 atccctcata gactgtctcg gaaccatcgg aggtgctttt tttcattatg ctgtacacaa
      301 aatcgttctt cgctgttatg acataggtat aatcagaact gctcctcaac tcccactttc
      361 caagcatagc cttgtcaacc ggaatatttg gtcgctcatc aagggctatt tcagcgctgt
      421 atgggcatcc agcaaacaag attcctgtaa taaggaacat cactatataa agacttcttt
      481 tcattttgct cgatttttaa caaatatagg cattcaggca atatttttac aggcttatcg
      541 tcaccgctat tatgatatgg acagcaatgg gcgccactcc ttcaggagaa tcgcttagaa
      601 gaatagcaag catgccgaac tataccaaag gtcagtttca aaatattgaa catacaccgg
      661 taatgtcgga agactaccat atgggtaaag tgatgatgga tatgatgaag cgaaattctg
      721 cccgcacacc tgcaacgcct ctccctaacg taaaaaccga tctgcttcat cttgaaaata
      781 atgcattggt ttggttcggg cactcagcat atctgctcaa aatcaatgga aagcatattt
      841 tggtcgatcc agtttttagc ggacatgcat ctccggttcc ttttatgatt aaagcgttca
      901 aaggatctaa ctcctatggt gttgcggaca tgcctgaaat tgatatgctc gtaatcactc
      961 atgatcatta tgaccatctg gattatgaaa cggttgttgc actcaagtca aaggtgaaag
     1021 aagttgtatg ctcacttggc gttgctgcgc atttgattaa atggggatat caggaatcgc
     1081 acattcatga gttgtattgg catgaatcga aaacggtttt tgaaaatttc acatttaccg
     1141 cagaacctgc ccgacatttt tcaggacgtg gaataactcg aaataaatcg ctatggtgcg
     1201 cctttgcttt gcagactccg gagcttactg tctttattgg tggcgatagt ggatatggaa
     1261 aacattttaa agaaattgga acgcgatata atggttttga tatcgcatta ttagaatgcg
     1321 gacaatacaa tcctgcatgg aaatatattc acatgatgcc tgaagaaact gttatggccg
     1381 cgcaggatat tcaggctaaa atatttgttc cggtacattg ggggaaattt gccttgagtc
     1441 tacatacatg gcaggaaccg gccgacaggg tagtggctgc tgcacagcga gcagaaatgc
     1501 catacgctac gcctaaaatt ggtgaagctt atgtaattgg tgccggtggg caaacgcatc
     1561 actggtggca aatgcgctaa gttgcggggt gtgattgtgg tgtgtttttt tcacgcagag
     1621 gcgcagagtc gcagagggcg cagggaggcg gaggggagcg gatgatttat tct
//
